LOCUS       DAORLF010000001        89889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_131_length_89889_cov_2.077087, whole genome shotgun sequence.
ACCESSION   DAORLF010000001 DAORLF010000000
VERSION     DAORLF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 89889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 89889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_89889_cov_2.077087"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..972)
                     /locus_tag="PK198_00005"
     CDS             complement(214..972)
                     /locus_tag="PK198_00005"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRF37140.1"
                     /translation="MGVIVLIIILWYSGLFFQSFFLHRYAAHRSFEMSKVTEKICFIL
                     TWVTQGSNYLSAYAYGVMHRMHHAFADTEQDPHSPKYDANAIAMMWRTWKKYAAIVRD
                     KVQIDEKFTKNVPQWKGFDKFASSWGSRLGWGAAYVVLFYFLATAWWQWLLLPVAFLM
                     APIHGMIINWFAHKYGYTNFKVDDTSKNLFHFDWLMMGEAYHNNHHKHGGRPNFGGVR
                     WHEIDVTYVIMLLLEKLRIIKLNRVAIPVKRERR"
     gene            1275..>2256
                     /locus_tag="PK198_00010"
     CDS             1275..>2256
                     /locus_tag="PK198_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37141.1"
                     /translation="MQKFDYPILYYSVNETTVLGLLPGTGLQVVEKDLKTVKAVLGDY
                     LLKQYKKSGLLPGRHLSNAKITAFDVNVRPSYNDNSVHYPMAGSLQLRLYAVIGNHSG
                     ADLECYIPWLEERFAVSQDMSRQVNALLQYTAETALRRLSPEEIFKLKSYDKPGLDRI
                     TMRVNTQRSRGWGGFGYRRSFPTLEQLTEQMPMRRPKAARSAVMPESAWEMEDVVNDL
                     AERIAVSRANVIITGSPGSGKSTVLLSAVRHITRNMKNSGLQLSFWRMLAQRITAGSK
                     YLGEWQETCETLIEELQACSGVLWVPDAAQLLLTGGSGPEDSVAAFMQPFL"
     assembly_gap    2257..2281
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2282..3566
                     /locus_tag="PK198_00015"
     CDS             <2282..3566
                     /locus_tag="PK198_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37142.1"
                     /translation="QLVGEATPQEVESMKRKLPGFTECFQVIRLPELSEKQVHSILER
                     FAAMALQQQNIGIQPDALKLAWRILHRYYPYEQFPGKGIRFLSRCVSDAVIAESETIT
                     ASDVVRQFVQQTGFPEMFLRDDMLMETEELARFFQQRIKGQEAAIQRLCRIIKVFKTG
                     LNNPHKPIANLLFAGPTGVGKTAAAKALADYFFGKGRSQTPLVRLDMSEFQSAWQIGR
                     LIGDGREPGQLVREIRERPFSVLLLDEVEKADPAVFDALLNALDEGILTDAAGRVTNF
                     RNTIIIMTTNLGANSGPPIGFAGERSAADSARAAILRHFRPEFVNRIDDIVVFEQLSK
                     AHIQAIAEKELEEFAQREGFISKSLHLTFSERVALHLTETGFDPVYGARPLQRALERE
                     VAAPLAAWLLKRPKLREATLRIDFDGGLKVDVDRQ"
     gene            3694..5328
                     /locus_tag="PK198_00020"
     CDS             3694..5328
                     /locus_tag="PK198_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF37143.1"
                     /translation="MKHKFIIGAVVLAAGLSIAASLSPVSDKFFEISRNIEIYANVYR
                     ELNMNYVDEVDPGKLMRTGIDAMVGSLDPFTNYISEADIESYRLTTEGRYNGIGASSE
                     KIGDYTTITELYQGFAADKAGLKPGDQIIAVDGKDAKGKDPEALNEIMRGFPGTSVSL
                     TIRRPGRNDDFKVSLVRAEVDVPNVPYSGMVGDHIGYVNLTTFTQDAGRNVGNAVREL
                     KNSDPNLKGVILDLRGNGGGLLNEAVNVCNTFIGKDLLVATNRGKIKEQDRGFSTFMP
                     AVDEDIPLVVLIDKKSASASEIVSGTMQDYDRGVLLGQRSYGKGLVQNIMEVGYNARV
                     KVTTAKYYIPSGRCIQSVEYKNGEPVDIPDSRRAKFTTRAGRTVLDGGGVSPDVALKQ
                     PADEPIVKALVNQHLIFDFVTQYCLQHPSVDSVDRFKFTDFDAFLKFVESRDFKYETD
                     SEKLLREFKDKAAKDGYKLDSEIAALEQNIRNAKKTAIAGYQTTILDLIEREIAGRYY
                     YKTGKIKMTLRNDSEVTEAKKVLLDTARYKSILKKQ"
     gene            5455..6468
                     /gene="obgE"
                     /locus_tag="PK198_00025"
     CDS             5455..6468
                     /gene="obgE"
                     /locus_tag="PK198_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRF37144.1"
                     /translation="MAEQNFVDYVKICCRSGAGGAGSVHFLRDKHTVRGGPDGGDGGR
                     GGHIILRGNRNVWTLLSLKYRKHVIAGKGVDGSGNNSSGAYGEDIWLDVPLGTVAKDA
                     ETGEIEFEITQHGEERVLLSGGRGGWGNSHFKTPTNQAPRYAQPGEPGREEWKILELK
                     TLADVGLVGFPNAGKSTLLSVVSAAKPEIAAYPFTTIVPNLGIVQYRDSRSFVMADIP
                     GIIENAHLGKGLGHRFLRHIERNSALLFMIPCDTEDISKEYDILVNELEMYNPELLDK
                     PRLLAITKCDLIDAEMQQLLLPELPDVPYLFISAVAQQGINELKDKLWEMVYRPEDDE
                     EEM"
     gene            6556..6807
                     /locus_tag="PK198_00030"
     CDS             6556..6807
                     /locus_tag="PK198_00030"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HRF37145.1"
                     /translation="MEQLVLRQLQQLPPKLQQEVLDFIGYLLSKQPMASEHTAPALRS
                     KELEEAIRIVRMGCDMRSFGDASNYQTASRTERSLPFRD"
     gene            6814..7182
                     /locus_tag="PK198_00035"
     CDS             6814..7182
                     /locus_tag="PK198_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRF37146.1"
                     /translation="MYLIDTNILIYSGESRYASLLLPYVTDTANGVSLISHIETLGFH
                     RITPAQITYFGSLFKILRVLPINDAIAKEAIQIRQNRKVALGDAIIAATALVYDVEIV
                     TRNTVDFTAIPHVKVINPIP"
     assembly_gap    7275..7373
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7374..>9364
                     /locus_tag="PK198_00040"
     CDS             <7374..>9364
                     /locus_tag="PK198_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HRF37147.1"
                     /translation="FYKQLDANDCGASCLRMIARYHGRYYSLDTLREMTYIDKQGVSL
                     LGISDAAERIGFQTLAAKLTYSRLCKDIPLPCVAHWNQEHFVVVHKVTERHAWIADPA
                     AGKFKISREQFLKSWAGDLENGEPSGILLLLQPTPEFYQRGETKADKGGLGYVWSYFR
                     QYSSYLLQIAAGLLIGVLLQIAFPFILREVVDSGVQSEDVQLIYAAVLGLFVLFVSQL
                     FVEYVRGWILLHVGARVNINLISDFLIKLTRLPLRYFDTKVTGDLLQRITDHERVQRF
                     LSSTSLFSVFSFFTFISFFMVLAVWNTGVFLLFAGGTLLSVLWFYLFSTYRRDLDYAR
                     FDQATDNQNKLMELINGMPDIKLHNAEKPRRWAWERAQAKLFHTALDTLRVDQIQKTG
                     AQFINEAKNIIILFWVALQVVEGHMTLGMMLGIQYIIGQLNGPLNQFVDFIRSYQEAR
                     ISLERMNEIHTKEDEERLEDKISLLPDWGDLILDRVSFQYAGPHSPLILKNVRLRIPK
                     GKTTAIVGASGSGKTTLLKLLLNYYQPNEGAVRIGDINLANIQNRLWRQKCGAVLQDG
                     YIFNDTIARNIALGDEVIDKYRLLQAVKAANMQTYVESLPLGYNTRIGADGQGLSLGQ
                     KQRILIARAVYKSPDYLIFDEATSALDAYNEMIVMENLA"
     assembly_gap    9365..9397
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9630..10910
                     /locus_tag="PK198_00045"
     CDS             9630..10910
                     /locus_tag="PK198_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37148.1"
                     /translation="MKFPIPALSDRKLLFLFAVVLAFLAFPMREWYQNGVIWADAEGY
                     YMYLPGVFIYNGFEKIPVRADGQFPHYPGTQKRFTKYTCGVAMMQTPFFLAAHARARA
                     EDKADGYSTYYIYGVLWAALFYAVAGVWILIKTLRRHFSQTVAWTAVMAVFWGSNLYH
                     YTVHEPGMSHVYSFFLVSCLLWLTPKLYKETIQRKWIFLIGLVAGLMVLIRPTNVVLL
                     LYPGLFGVGSWSDFKARLQFFGRHIGQLWPAALLILAAWIPQMLYWRYMSGNWIMYSY
                     GDEGFIYWNHARMAQVLFDIKNGWLLFSPMMAIALIGMVAGLWRNRYNERWIGLAWLI
                     SWYLFASWWAWWFGGAFGHRSFIDLYPLLAIPFAAVVQAVWSTRSIVLKGLFLLLLGA
                     LAYYGLGLTSHFISPHYEWPTWQAAIELMLKGKF"
     gene            10954..>11484
                     /locus_tag="PK198_00050"
     CDS             10954..>11484
                     /locus_tag="PK198_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37149.1"
                     /translation="MRSEADHIELRSEEVQEILGTPPGWLARWGTLAALIGVVALGWL
                     GFWLEMPQTLEADVKITSKDPPRRFYAPSTTVLNDIIFGNESKVDSGATILHFNSKAR
                     LAHVLSLETELLQVGNDQQILSLNIQRDLILGELQEDYYDFLEKWDQYHFVSNRSTDN
                     SGRSEVASRIRDLESEI"
     assembly_gap    11485..11530
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11531..12236
                     /locus_tag="PK198_00055"
     CDS             <11531..12236
                     /locus_tag="PK198_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF37150.1"
                     /translation="AQARYDKELTLLNRGVLTREELNPTRIERDSYERQVQDVQSSIR
                     SKSNQISMLRDRSRSNSGRAKALEDQSAAFGLLKDSFARLRNRVSEWKREYTVESPIS
                     GTVLFAKDNLAKGQFVQRDSLLLVIVPGKASGTVAKMEIVPEEAGQIVRGQKVQIKLY
                     DYPFPDYGAVWGQVEGKGKLPSGGKIPVEVRFPGDSLVTTKAGLIQPEGEMRGKATII
                     LSRRRLIQRIFGIGEG"
     gene            complement(12189..12776)
                     /locus_tag="PK198_00060"
     CDS             complement(12189..12776)
                     /locus_tag="PK198_00060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HRF37151.1"
                     /translation="MQAKELIRLSKFLSLVLRHRPQLIGIILDEQGWTDVPTLLQKAN
                     AAGTPLDIETLRFVVENNNKKRFVLDETKGRIRAAQGHSVEVALGYSAQEPPEILYHG
                     TAERFLKSILESGLQKRQRHHVHLSRDVETARSVGSRHGRPVILEVRGGAMQNAGFQF
                     FISENGVWLTDEVPAEYLRVLSFPDAEDALNKPPA"
     gene            13186..14331
                     /locus_tag="PK198_00065"
     CDS             13186..14331
                     /locus_tag="PK198_00065"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRF37152.1"
                     /translation="MRFGTKAIHAGVEPDPATGAVMTPIYQTSTYAQEAPGVHKGYEY
                     ARTQNPTRHALEQNLAAIENGTEAFCFGSGMSAMDAVMRMLNPGDEVIATNDLYGGSY
                     RLLTKVYERFGVRSHFTDMTDPANVEALINSNTRLLWIETPTNPMLNIIDIEAVCKIA
                     RQHGILTCVDNTFASPFLQMPLDLGADIVVHSATKYLGGHSDVVLGAVIVKDEALAQQ
                     LRFLQNAVGAVPGPHDCFLVLRGIKTLHLRVERACKNAKKVAKFLAAHPKVQHVYYPG
                     LKDHPGYMIASRQMRFYGAMVSFILQKDTVDDAMKVLSGTQIFTLAESLGGVESLIGH
                     PASMTHASIPREERLKTGLSDSLIRLSIGVEDFEDLIEDLEQALRLV"
     gene            complement(14847..15467)
                     /locus_tag="PK198_00070"
     CDS             complement(14847..15467)
                     /locus_tag="PK198_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF37153.1"
                     /translation="MMKRFFLLLLPVLGLAAASCQHPEGKLPILGEIDIVDGDTLYHK
                     VPDFSFVNQDSQEVTNATFADKIYVADFFFTSCPTICPKVKRQMLRLYERYENEDRLV
                     LLSHSIDVRRDTVGRLREYAAGLGVSAPRWHLVTGDRAAIYDIADDYFSVAIEDATAP
                     GGFDHSGRLILVDTKRHVRAFCDGTDPKSVDQFMKDIDRLLKEEIR"
     gene            complement(15464..15922)
                     /locus_tag="PK198_00075"
     CDS             complement(15464..15922)
                     /locus_tag="PK198_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37154.1"
                     /translation="MNFKMILLALAFLTGLSACGTNQAQEAEQQKLWDEMMVVHDEVM
                     PKMSDINALSKQMEAATADTALTDELRDATRKALYDLQAADKVMWDWMYGLQQLPDLR
                     NGKKHEDIMQYLQDEIGKITEVKNAMLGSIEAAEALKKQLPQNASETNAQ"
     assembly_gap    15997..16075
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16114..>16467
                     /locus_tag="PK198_00080"
     CDS             16114..>16467
                     /locus_tag="PK198_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRF37155.1"
                     /translation="MTRLSVNINKIALIRNSRGGNMPDLVQIAKDCEAFGAQGITVHP
                     RPDERHIRFSDIPLLKAVTTTEFNIEGNPTEAFLKAVLEHRPHQCTLVPDAPSALTSD
                     AGWDTVRHASFLKEVC"
     assembly_gap    16468..16497
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16498..>16745
                     /locus_tag="PK198_00085"
     CDS             <16498..>16745
                     /locus_tag="PK198_00085"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRF37156.1"
                     /translation="RSSIFINADEKMVEGAKALGADRVELYTGPFAAQFPQDPEAAVA
                     PHRRCARIAAEIGIGLNAGHDLNLDNLRFYQQHTEALL"
     assembly_gap    16746..16755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17057..>17943
                     /locus_tag="PK198_00090"
     CDS             17057..>17943
                     /locus_tag="PK198_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF37157.1"
                     /translation="MKKLSLVLALVLFAFGAAVAQRTVSGMVSDTKGEPLIGASILVK
                     GTVTGTVTDFDGKYSIAVPAGSTTFVVSYTGFSTKEVEIGASSSINITLEDAAEQLSE
                     VVVTGLGIRKEKKALGYAVTTLGSRDIELRPEADVSRVLRGKVPGVDISQTSGLAGSG
                     TNVIIRGYSSITGTNQPLFVVDGIPFHSSTNADRGFDSGSASASSRFLDLDPNSIAEV
                     SVLKGLSATVLYGEAGRNGVILITTKNGVPANSNKKLEITVNQSLYVNQIASLPEDQD
                     LYGNGFDNFASAAFSNWGAP"
     assembly_gap    17944..17999
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18000..20233
                     /locus_tag="PK198_00095"
     CDS             <18000..20233
                     /locus_tag="PK198_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37158.1"
                     /translation="YDRAALNAVFPQYIGARYAYKAYDNLQNFFTKGLINNTAINIAS
                     QLANNTTLNVSYAFRDDTGFIPQEYNRYTKHNLGLGLQTKLSNGFKISSTFNYVTSDR
                     ISPPSDISFSSGSDGSFTSLFGHIFYTPRSVGLYELEYENPVDKSSIFYRGGNDIQHS
                     LWTLNNSGDLEDLSRFFGNMQVSYELTDWLTAAYRVGVDGYSQQQRYYLNKGGIDVPT
                     GVLRTSNRSNTILDHLFSLAYDFRLGDSWVLDGVAGFNMRRDVLNRTFLNSSQQFVYG
                     LFTHDNFIDNTSSSDKFEENLMGAFLTASLGYKSFLYFNFQARNDWTSTLEKENRNVF
                     YPSGSVSFIPTEAFAGLQNSNLLNYMKLRFGYGTSAGYPDPYQTRNILSTGTRIFVND
                     GGTVINTNSVSNVFGNPNLRPELHKELEFGLEARMFKNRLGIDLSLYNKTSSDLIIDQ
                     PLDPSTGGTSTTVNAASIQNKGIELGVDITPIQSKNFTWTLNANFTKNQGIVTELLDG
                     VDQIAFSGYSNLGNFAIPGRPYGIILGDKILRDDNGNPIVTSAGVYQSSGEIGELGDP
                     NPNYLLNGGTSINFKGFSLSALLAYVDGGVIYATTPSTLMGRGVLQETGFDRFVPLIA
                     PGVKADGTPNDIMINASSHYWENGGVFHDEMRTYDASYVKLREVSLGYSFPKKILEKT
                     PFGSMTLVFSGQNLWFRALGVPKGANFDPEVSSLGVGNGRGFELMNVPTSKQFGGSLK
                     FTF"
     gene            20259..>21069
                     /locus_tag="PK198_00100"
     CDS             20259..>21069
                     /locus_tag="PK198_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF37159.1"
                     /translation="MKRLFKISIIASFLIFLGACESTELEGLLNNPNAVTPENAELDL
                     VFNRVMLDFKSFVYEAHAQTAPYTRMLAMTGGYQYNSQDQPQSFDYIWELAYANLLPD
                     IELVISSATEKGSTVHAGIAKILKAYVYMTLVDLFGDIPYSEALGGASNPSPKADDDK
                     AVYEAAAAVLAQGIDNLKTPVGSPVNDLYYNKNVARWIKLGKTLQLRYHVMTRLSGGS
                     ATVVNALLAEGDLINSADEDFQFKYSNNRSNPDSRHPAYSGAYESAAGAYLS"
     assembly_gap    21070..21085
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21086..21921
                     /locus_tag="PK198_00105"
     CDS             <21086..21921
                     /locus_tag="PK198_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF37160.1"
                     /translation="FFADKTTEDPRLRYYFYRQDCDEREEDLFTLGCRTQPYPFHWAD
                     GYPFCTAGLMFSGANNQYLGYWGRDHGDASGIPPDNQKRTAIGVYPAGGKFDANNCAH
                     VQRLGVDGLKGAGIQPIMMSSWVDFLKAEAALTMSGVAGDPRSLLESGVRKSISKVMA
                     FGAADATSAYVPSTAQIDAYVAEVLADYDAAATTDAKLNVIIKEYHLALWGNGLEAYN
                     NYRRTGKPAGMQPTLQADAGTFPRLLWYPANYANLNANADQRNLAQQVFWDTNAAGFI
                     K"
     gene            21992..22822
                     /locus_tag="PK198_00110"
     CDS             21992..22822
                     /locus_tag="PK198_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37161.1"
                     /translation="MKKILISFFSLSLLFLAFSCEQERAFPEFEDLQYGSFPRRLSFT
                     GEYFITNIATSKITFKVEFYDENQGKNVESYSWAVSYIDRTNNGANNRPAQTIRTVNA
                     SQFVTNPDTGLPSTTIEIPLQDVLNLFGFSANDVNGGDSFIFNATLRRTDGKVFTSVN
                     TNDLLEGQPAFNAFFQFRADLKCTPAALAGTYNYSTVGWCGTTQTGTTRLVATSVSGV
                     FNVVSPDAPSDPDAADFSFGAYAACYGAGSTLPGGNLQIVENCYGLRPIGLSRWGEDY
                     "
     gene            23199..>23423
                     /locus_tag="PK198_00115"
     CDS             23199..>23423
                     /locus_tag="PK198_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37162.1"
                     /translation="MTSFTAGEPSPGYNLIFPHNQPHVYLLNNCGQIVHRWNSPSVRR
                     PGNTAYLLPDGNIVIASRYFASVNDPIWAGG"
     assembly_gap    23424..23436
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23437..24796
                     /locus_tag="PK198_00120"
     CDS             <23437..24796
                     /locus_tag="PK198_00120"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HRF37163.1"
                     /translation="GEYVEIRNWDNDTLWSFTLNDTLRRLHHDIAPMPNGNILMIAWE
                     LKTFEEAVQAGRNPALMPQHKFWPDYIFEINPATNNIVWEWHVWDHLIQDFDPTKDNF
                     GSVAAHPELVDLNYDDNGGNPDWMHSNAIDYNPVLDQIALSVPTFNEIWIIDHSTTTE
                     EAAGHTGGRSGRGGDLLYRWGNPSVYGAGDSTHQRLFYPHDIHWADDFLDASHPHYGK
                     LVAFNNQVGPNFSSAEIFNPAFDTLTWSYPMAEGRWLPVQSDQTVTHPVPTRMYSDGT
                     SSIRLQPNGNILLCSGRQGYAFELNEELDIVWEYITPLVGGVPIAQGSTATNNLTFRM
                     ERYPESYPGLEGKDLSPKGYIELNPDTTFCSLISSIGETDTEMPDWKLYPNPAGDELW
                     IEWNGDAPAFLEFFDAMGRLQHKTKPVGNTPTRLSVTDWPSGVYYLSDSRGNTARFVV
                     RR"
     gene            complement(24828..26606)
                     /locus_tag="PK198_00125"
     CDS             complement(24828..26606)
                     /locus_tag="PK198_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF37164.1"
                     /translation="MKQFFITSACCFGIVISLHAQILYQDAAAARGIQHIYNSTTPGC
                     GVSCVDFNNDGLDDITLGSESGQPIRFYLNMGGQFTLLQPLVPHLDEVKQLLWVDFDN
                     DADKDLFVCNYEGVNRLYRNTGNLTFEDITLSAGLPLDAFHTFGAVWGDYDRDGLLDL
                     YYNERKLQLSGLPNENRLFRNNGNGTFTEVTPQAQAADSGKTPFCSAFLDYNNDKWPD
                     IYTAQDRLRINSLLRNNGNGTFSNVSVATNTNLAMNAMCVAVGDYDNNGWLDIYISNT
                     EEGNALLRNSGPDDDGQVVFEEVAASAGVAFNSIGWGSVFLDADNDGDLDLYVSGMLA
                     GADVISSAFYENMGNGSFLQPNAGFVGDTVTSFSNATGDFDNNGYPDIMVANTAPFAS
                     QLWLHQGTTSNNWLKIKLQGILSNRDGIGAKIEIYTGSHYQMRYTHCGIGFLGQNSDT
                     EIIGIGSATQADSILITWPTGHIDRLLNVVSGQKLVVVEGSTTGGDIFVDPEIISHTS
                     DLPADQPQAAHLFPNPATDELHVQLIGNDIRFWSVLDTYGRVLMSDRIRAAEWNIAVQ
                     HLSSGLYFILFLQEDGQVIMQKWIKR"
     assembly_gap    26655..26683
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26684..27126)
                     /locus_tag="PK198_00130"
     CDS             complement(<26684..27126)
                     /locus_tag="PK198_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF37165.1"
                     /translation="MQKDRKEKNFIRRPIYPGGPKAMQEFLRQNLRYPAEAFKKGIEG
                     TVSIRYEIDYKGNVTDVEVLTSVGHGCDEEAVRLAKLLKFDVPPIRKLKVTFHNTLKV
                     HFRIQKNVAAAEPAPVVTAPSPQVVYNYVSAPPEPQKDTTEQKPAT"
     gene            27281..27508
                     /locus_tag="PK198_00135"
     CDS             27281..27508
                     /locus_tag="PK198_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37166.1"
                     /translation="MNNEITHAPQGADQQPPAPGSNIWGPKFPLFGLAVILLFALLIG
                     ARACYLGVPLGDVFKNTDPAPTTIDTTSTGQ"
     gene            27505..28302
                     /gene="dapF"
                     /locus_tag="PK198_00140"
     CDS             27505..28302
                     /gene="dapF"
                     /locus_tag="PK198_00140"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRF37167.1"
                     /translation="MKRSIPFKKYQGAGNDFVIVDNRAETFIGRFDTELIQRMCDRRF
                     GIGADGLMLLQNHPGYDFEMVYFNSDGRESTMCGNGGRCLAAFAASLGFLNENRCHFI
                     AIDGPHDAVIVRPDWVELRLGDVREIEKGAGFFCLNTGSPHYVRFVEDARHLDVNAEG
                     RAVRYSERFVAEGINVNFVEETAEGLLVLTYERGVEAETLSCGTGVTASALAYSLKMQ
                     QKGAVSVPIITKGGRLEVKFQAQDEGFSDVWLCGPAVEVFEGVYWQQ"
     gene            complement(28405..29430)
                     /locus_tag="PK198_00145"
     CDS             complement(28405..29430)
                     /locus_tag="PK198_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37168.1"
                     /translation="MIPPTSSSTPGAPMGSSGPDNSRQRLIAVTAVIIVALLGVIAFL
                     LVNKFKQDRQSTELTTQLSESETLKAELEKQYYDALSQLEEMKGSNSELNARIESQME
                     ELSSQKAKIDQLLAGKGSLDKARSEIKKLNGQVEQYLAEINQLRQENLALGDQNRQLS
                     EEKQTLTGDLEAQRASNQELASAKEALAGEKARVEQERTVLAKKVDRASMIAVQGVEV
                     TGLKTRDGGKSVKKNYADNIDQLQVCFNTTANDVAENGKETYYIRIISPLGETLAVEG
                     LGSGAFTAADNDQIRYTMSKEFNYSGTPQKMCSVWSPGQAFSKGKYEVEVYNKGYLAG
                     KGSVVLK"
     gene            29524..>30051
                     /locus_tag="PK198_00150"
     CDS             29524..>30051
                     /locus_tag="PK198_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37169.1"
                     /translation="MRILTLGLTFSELPYIYTSIQPHIMIERIDIANVLFLDIETVSA
                     TASYEDLDENFQELWFHKTAGILRKPEDELTEEEADIAYRRRAAIYAEFGKIICISVG
                     FAFHDGEEIKVRLKSFAGHDEAAILKQFTELLEKSYNDTNNKYLCGHNIREFDIPYIC
                     RRLVVNGLPMPAPLQL"
     assembly_gap    30052..30076
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30077..30335
                     /locus_tag="PK198_00155"
     CDS             <30077..30335
                     /locus_tag="PK198_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF37170.1"
                     /translation="LDTMELWKFGDNKSFTSVKLLSALLGFPSPKDDIDGSMVGSVYW
                     EEQDVDRISLYCEKDVLATMQLFLKYQRKPILESSQVMHVR"
     assembly_gap    30422..30431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30432..30877)
                     /locus_tag="PK198_00160"
     CDS             complement(<30432..30877)
                     /locus_tag="PK198_00160"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HRF37171.1"
                     /translation="MPPIVNEKRQYPIGRFTFNPIFSPSELAAHIERIRTLPIRLNEV
                     LSQMDASNWDTPYRPGGWTARQVVHHIADSHINAFVRFKLALTETTPAIKPYLEEQWA
                     ELPDSRQLPPQVSAQLIAALHERWTVLLQSMQPSDWKKTLYHPERQR"
     gene            complement(30917..>31383)
                     /locus_tag="PK198_00165"
     CDS             complement(30917..>31383)
                     /locus_tag="PK198_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRF37172.1"
                     /translation="LEDISVSSPRMFDNEDEAKKYRRYRRYAADVYPYAVEAIKVFHE
                     VQDATEDMRRGKAKRYAKHLYKDMKDNFEDPLKNLTKTQGMILIKMVERELKTPTYFI
                     IKDLRGNWTAGYWGTVGKMFGHNLKEGYIPGKDRILDMVLDDFNVSYKMPSK"
     assembly_gap    31384..31549
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31550..32964
                     /locus_tag="PK198_00170"
     CDS             <31550..32964
                     /locus_tag="PK198_00170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF37173.1"
                     /translation="HNKTLMPAFLAGAVLVLQSINNKKRYCVERKPTELYLYGHHFTI
                     INAMKQFFFLTIAFLLAFQFSFAQKLAETKPEKAGMSTERLDRIGQVVQSYVDSSYIP
                     GGVALVARHGKIVYYKAFGFADIESRRPLQRDDIFRIASQTKAITSVAVMMLYEEGRF
                     LLDDPVSKYIPEFAKPQVISQYDAADTTYTTVPAKREITIRDLLTHTSGIGYAGIGTP
                     EANAIYAKNNIPSGIGTPDQNLGEAMRRLGALPLFHQPGEKWTYGLNTDVLGYLVEVL
                     SGMPLDEFFRRRIFQPLGMNDTWFYLPADKHARLTKLHTENKEKKAVLFPNSTLDPDF
                     PKLQGKFFSGGAGLSSTAYDYAVFLQMLLNGGTYNGVHILSPASVRMMTSNQIGDKYI
                     GAVKKFGLGFGLTTEAEAARVPPSVGTFDWGGAFATTYWADPQEGIVAMFFTQKYPHS
                     YGELATKFRVLVYQAVKAGR"
     gene            33105..33566
                     /locus_tag="PK198_00175"
     CDS             33105..33566
                     /locus_tag="PK198_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37174.1"
                     /translation="MKTSFLPLVLLLSFFATTQIQATADTGNMPQGAVHKMLEGKWQV
                     TINADELPQAAREENAAPDNADFAVITYRFADNGALTRSVQRGAARWEESGEWQLTED
                     GRYISFHFSGKAAVQAEIKYIEADEMVLAQPSGATDSNLQNEVRQLYFTKM"
     gene            33622..>34475
                     /locus_tag="PK198_00180"
     CDS             33622..>34475
                     /locus_tag="PK198_00180"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRF37175.1"
                     /translation="MKPLAERMRPLTLDDYLGQQHLVGPGAVLRKSIETNRLPSFILW
                     GPPGVGKTTLARIVAHQLDRPFFTLSAVHAGVKDVRDAIEKAKGQRFFDRPSPLLFID
                     EIHRFSKSQQDSLLGAVEEGVVTLIGATTENPSFEVIPALLSRCQVYVLQPLGKEDLI
                     HLVDQALEKDEVLKTKPITVEEYDALLRFSGGDARKLYNVLELVCHFQTGEEPMLITD
                     SLVAQLIQENLVMYDKGGEHHYDVASAFIKSIRGSDPNAAVYWLARMIEGGEDVKFIA
                     RRMIIAAA"
     assembly_gap    34476..34526
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34527..34886
                     /locus_tag="PK198_00185"
     CDS             <34527..34886
                     /locus_tag="PK198_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRF37176.1"
                     /translation="QAVGLPEARIILSEAVIYLATSPKSNSAYAAIGAAQEEVRRSGH
                     LPVPLHLRNAPTHLMKKLDYGKNYLYAHDYPGNFVEQEFLPEEISGKTLFSPQSNASE
                     EKIRQFLAARWKKRYGY"
     gene            complement(34889..36304)
                     /locus_tag="PK198_00190"
     CDS             complement(34889..36304)
                     /locus_tag="PK198_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF37177.1"
                     /translation="MDILHSAYNPEHFREQGHALINLLADYLTDAQSSRPSLPVIPWR
                     APDEQYEHWKNFLAVPGSTEDFFHKILKDSIHLHQPRFMGHQVCPPAPLSALAGLLGG
                     LLNNGMAVYEMGGPATAIERLLIEKTAQVLGFDARQAGGFLTSGGTLANLTALLAARS
                     RYAGAHVWKDGHQKPLALMVSAEAHYCVDRAARIMGWGDAGIIKVPVNERFQMRTDLL
                     EMLYRQALEQGVEVIAVVGSACSTSTGSFDDLAAIGRFCHSRNLWFHVDGAHGAALAF
                     SRQHRAVLAGIETADSVALDYHKMLMTPALTTAVVFRQDADSYGTFSQQAQYLFGETE
                     REWYNVAKRSFECTKLMMSIKAYSLLYTYGETLFENYVDQVMENGRIFAAMIEAQPQW
                     ELAVQPQCNIVCFRHAPPGLSAEATDEHNLRIRRRILEDGRFYIVQTAINGHTWLRVT
                     LTNPFTSAVEMEDLLGFCEKI"
     assembly_gap    36613..36664
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36665..>37120)
                     /locus_tag="PK198_00195"
     CDS             complement(<36665..>37120)
                     /locus_tag="PK198_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRF37178.1"
                     /translation="ALIITGIVLFIFATQFNKRTPAGNESYRRMEGFRLFVKKAERPV
                     IERLMQEDPLYYDKTMPFALAFGYLKQWNAHFDGLLSQPPSWYGGSGVYGHNATNSWT
                     NFSESFPKEVNQIGSVFSSSPSSSSSGGGGEQSVAMKLWPEARIRDEDFS"
     assembly_gap    37121..37160
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37161..38339)
                     /locus_tag="PK198_00200"
     CDS             complement(<37161..38339)
                     /locus_tag="PK198_00200"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRF37179.1"
                     /translation="MRLRNCLLIAALCLLVLPAKAEFFNIVNYDVNVKLSSEGYADFE
                     EVIEVEFTTSRHGIFRQIPLRSEINGKMVTRIIRDIEVDGFKFTTSKENGNLILKIGH
                     KDIWVEGRQTYRIRYRVLNPLNFFEEHAEFYWDLLGISWPVVTRKFSFSIELPGGIRL
                     AKNDVQYYTGYNGAQGQDAVVDVSANYISGQTNREFSAGEAVTVAVKLPRHAFKKMDD
                     WTAFRLQHSLLLVPFFFLAAAWAALRRARNRKETIMVEYFPPEGVSPAIVGGFVDHSV
                     DQSDVLCLIPHLANQGYLRLEMEEKSGLFGKDKITFYKLKEAGDELFAFEKKFFNALF
                     STGDVVYLESLKDKFYMHISAVQTEVKSWIKAQGWYQADQEKLGCGVGVAGAAAMAWG
                     L"
     gene            complement(38345..38893)
                     /locus_tag="PK198_00205"
     CDS             complement(38345..38893)
                     /locus_tag="PK198_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF37180.1"
                     /translation="MSTLLIIVGILLLAAFLLAGTYNKLVRLRNGAEEAFKSIDVFTK
                     QRYDLIPNLVNTIKGYTQYESSTLEKIVTLRNSAMSAGGKDGGAADSALSGALKSVFA
                     LSENYPDLKANQEFLNLQNKLSELEESIQRARRYYNASARDMNNAVETFPNNLIANAF
                     GFRALDYFEVAQEETKNVNVQF"
     gene            complement(39066..40394)
                     /locus_tag="PK198_00210"
     CDS             complement(39066..40394)
                     /locus_tag="PK198_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF37181.1"
                     /translation="MELLAREIEAAGASIINTGIGWHEARIPTIAMMVPRGGFSWVTQ
                     RLMGKVNIPLITSNRINTPETAEHILTSGHADMVSMARPFLADPEFVKKAAQSRADEI
                     NTCIACNQACLDHTFKRKTASCLVNPRACNETTLNYLPTTQRKRIGVVGAGPAGLAFS
                     TIAAGRGHEVHLFDNEADIGGQFNMARRIPGKEEFNETLRYFRKQIELTGVQLHLQTR
                     FDAEMHRQYQFDELVLATGISPRKLNIEGIGHPKVLSYIDVLKHRREVGRRVAIIGAG
                     GIGYDVASYLLHEHSETSQSAELFMQYWGVDMQYAERGALTKPAPTKPEREIFLLQRS
                     AGKPGEKLGKTTGWIHRSDLKRHKVQSISEVQYVKVDDAGLHITVKGQPRLLEVDHVI
                     VCAGQEPLRELQLPLESAGVKVHLIGGALEAGELDAKRAIDQAARLAAGV"
     gene            complement(40298..40786)
                     /locus_tag="PK198_00215"
     CDS             complement(40298..40786)
                     /locus_tag="PK198_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37182.1"
                     /translation="MCMQILHAGRYGYHPLCVAPSGIQSPISPFKPWTLTASGVERQI
                     RHFVRCALLAQEAGYDGVEIMGSEGYLINQFIAARTNHRTDRWGGPFENRIRFAVEIV
                     RRTREAVGTNFIIIFRLSMLDLRAAPGKRWNCWPARSKRPAPASSTPASAGTKPAFPP
                     SP"
     gene            complement(40852..41082)
                     /locus_tag="PK198_00220"
     CDS             complement(40852..41082)
                     /locus_tag="PK198_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37183.1"
                     /translation="MMDTKYPHLLQPLDLGFTQLPNRVLMGSMHTGLEESPGGMEKMA
                     AFYAERAAGGVGLIVTGGIAPNRAGWRPLPAN"
     gene            complement(41152..42858)
                     /locus_tag="PK198_00225"
     CDS             complement(41152..42858)
                     /locus_tag="PK198_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37184.1"
                     /translation="MKLPYGQSNFLNVATEFYYVDRTQYIETLEQQANRYLFFLRPRK
                     FGKSLFISMLEYYYGLQHKDDFGRIFGKYYIGQHPTPLANSYLVLTLDFSGISTDNRE
                     LVLDGFNLAVKTGVERFMVNYGKGQITAEDRRQVEEENLPADIIQRCFNFAYRLWPDR
                     KLLILIDEYDHFANELIAFHLEDFKEIVGRNGFVRKFFETIKKESRNGLVERMFATGV
                     SPITLDSLTSGFNIAEKIGTELELHNMMGFTEEEAVDLLAFAGVSQSEISAMIADLRN
                     WYDGYLFHPDAETHLYNPNMLIYFANAWQRHKKYPEKLLDDNIASDYGKIRRLLGVGG
                     EEMSLSILEDALEKDFVRANLTGMFSFDRDWVRDDYTSLLFYLGVLTVRNRAGSFWNF
                     TIPNFVIKGLYYSYFLDTLRRRAELKETVFTDINDAFYTLSMDNELQPMIEIVERILS
                     RLSGRDVQHFSESHLQVIWAALLNTSQAYLVRTEHETERRFVDILCAKLPQIPVNWSF
                     AFELKYLKKSESKQLTAKAEEARAQLEAYLQTDDLRRIPNLAAYAIVFVGPKAKSVQR
                     VQ"
     gene            complement(43077..43706)
                     /locus_tag="PK198_00230"
     CDS             complement(43077..43706)
                     /locus_tag="PK198_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37185.1"
                     /translation="MYIKLRYRGRLRQPSEVYQLIQEVQDICTANAWNYKVWDEDWSL
                     PSTLDMAFTGTAMNFTGNAPLKGITLNIGDTETAWLTFQPDGTLQSFFTLVNPLFIGN
                     DEDFPWQRVKTGNDGPETHIALCKLFHYLADKYFLVFEVQDESGYWGSGNEEQFSSWL
                     QDTEIATRQLTEELELIRTNTAWSEAEREEAAKRLVADMRERLKAGGAG"
     gene            complement(43720..>43985)
                     /locus_tag="PK198_00235"
     CDS             complement(43720..>43985)
                     /locus_tag="PK198_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRF37186.1"
                     /translation="RYVFRLVAIKEILANPQAFGFVLEEKDKYSPLDEYKVVDVKGPV
                     AHWPDFAQEQGISYRMLRLYNPWIIAPKLTNKAGKTYEVRIPK"
     assembly_gap    43986..43995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<43996..>44326)
                     /locus_tag="PK198_00240"
     CDS             complement(<43996..>44326)
                     /locus_tag="PK198_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRF37187.1"
                     /translation="LKENGIPDDFKYTAVAESNLRNAVSPAGARGIWQFMAPTAQQYG
                     LEVNDEVDERYHPEKATAAACKLLRDYHRQFGNWTLTAAAYNIGGPRLGREKEAQRGK
                     TFYDLQLN"
     assembly_gap    44327..44348
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<44349..44648)
                     /locus_tag="PK198_00245"
     CDS             complement(<44349..44648)
                     /locus_tag="PK198_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRF37188.1"
                     /translation="MQKMLYVYSLAVTLLLAAVLLVAYSGGENAPDGVKKLVSAFKEL
                     PQNIQPVSLDRDFDFAGEALPMNNFDVRERLDRELTVNAYTHGTTLLNIKAMYRYF"
     gene            44743..>45267
                     /locus_tag="PK198_00250"
     CDS             44743..>45267
                     /locus_tag="PK198_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37189.1"
                     /translation="MTADNLLQFLRQPQDLQRVSYQELKTLALEYPFCPHVHQLLLFK
                     SRLSGHKDYAADLSRTAAHSVDRRYLRSRLEELDAALAAVQEEVFELKPVAEIEQQLA
                     VRKEPNAVELSPSALRPPRHSAAADDRPDQAAAPEAIPAPSPVPRPKRRMPAGHTTDD
                     LPGDVPQPLQKDAFR"
     assembly_gap    45268..45487
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            45618..>45841
                     /gene="secG"
                     /locus_tag="PK198_00255"
     CDS             45618..>45841
                     /gene="secG"
                     /locus_tag="PK198_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRF37190.1"
                     /translation="MSILTVFIAFIGILLMIAVLIQNPKGGGVDSTFGGAQANQMFGA
                     AKSSDFIEKATWGLAAALLVLCLVAAVLVG"
     assembly_gap    45842..45940
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            45958..>46296
                     /locus_tag="PK198_00260"
     CDS             45958..>46296
                     /locus_tag="PK198_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4269 domain-containing protein"
                     /protein_id="HRF37191.1"
                     /translation="MYCFDDIAYLQQGSDKQRRVWELLTRHCIFEKLALFDPLLTGTI
                     PIGIDTPDSDLDIICHCPDVPHFESVLRSLFGTEQHFRLHCKVIGGRTSVVCSFEKEG
                     FMIEIFGQDQP"
     assembly_gap    46297..46320
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <46321..46503
                     /locus_tag="PK198_00265"
     CDS             <46321..46503
                     /locus_tag="PK198_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4269 domain-containing protein"
                     /protein_id="HRF37192.1"
                     /translation="RLQNAYRHLLIEARLLEERGPEFRQQIIELKQQGVKTEPAFARL
                     LGLEGDAYEALLRLES"
     gene            complement(46500..47429)
                     /locus_tag="PK198_00270"
     CDS             complement(46500..47429)
                     /locus_tag="PK198_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRF37193.1"
                     /translation="MSIEIETLQQLLPSVDEDFVAAVEAVFASRGRLIVTGIGKSAIV
                     AQKIVATLNSTGTPAIFMHGADAIHGDLGMMLPDDVVLCLSKSGETPEIKALVPLVKR
                     RGNLLLGMTAQRHSFLGQQSQYVLHTPVSREADPNNLAPTASTTAQMALGDALATALL
                     ALRGFSPDDFALFHPGGALGKQLYLRMSDLYPANEKPAVRPADSIRAVIIEMTSKRLG
                     ATAVTDENDQLLGIITDGDLRRMIERSTNGLDHILAADIMTPSPISVSPDMQATEAFE
                     LMRLRSITQLIVAENKRYLGMVHLHDLIREGLI"
     assembly_gap    47431..47594
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47712..>49301
                     /locus_tag="PK198_00275"
     CDS             47712..>49301
                     /locus_tag="PK198_00275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRF37194.1"
                     /translation="MTLTNELTLHDALQRYFGFDNFKGMQEQIIESLLRGEDTFVIMP
                     TGGGKSLCYQLPALMLDGTAIIISPLIALMKNQVDSIRGYSQSDDVAHFLNSSLTKAQ
                     MKLVKQDISDGKTKLLFIAPETLTKDENIEFFQQVDVSFVAVDEAHCISEWGHDFRPE
                     YRRIRSMLDAIGKEIPIIALTATATPKVQSDIVKNLNMRTHNTFISSFNRENLYYEVR
                     PKGKPEQTLKQIVQVIKTMPGQSGIIYVQSRKAAEDIAKTLVVNGIKAAPYHAGLDAK
                     TRTQTQDDFLMEEIDVIVATIAFGMGIDKPDVRFVIHFDIPKSIENYYQETGRAGRDG
                     LEGRCIAFYAYKDILKLEKFLRDKPVAEREMGAQLMQEVMAYSETTACRRRFLLHYFG
                     EEYDDAQCNDMCDNCRHPKARIEVKKEMVMALRSVKELDENFAIKPIIDFVIGHETKE
                     IKDFGFDKKELFGAGKDHDDNFWSSVLRQAMLNGFINKDIESYGLLKMTEKGHHFIKN
                     PQPFQIPLNHDYDKELPESDEP"
     assembly_gap    49302..49347
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49348..49913
                     /locus_tag="PK198_00280"
     CDS             <49348..49913
                     /locus_tag="PK198_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRF37195.1"
                     /translation="DLRRREAKRLNVPPYVIFQDPSLEDMAIQYPVSMEDMAKVSGVS
                     IGKATRYGKAFVELIRKYVEENEIERPSEVVVKQMANKSKAKVNIIQNIDRKISLDDV
                     ARATGLNMDELMDELDAIVESGTKVNIDYYLEDALDEAVREDIYDYFMQSDTDDIKAA
                     YKDLREDEVTMEEIRLVRIKFLSEMAN"
     gene            49974..50213
                     /locus_tag="PK198_00285"
     CDS             49974..50213
                     /locus_tag="PK198_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRF37196.1"
                     /translation="MNPLLERISVNPQVCFGKPCIKGTRIWVSLILDFLATGTGIEEI
                     LQHYPQLQREDVLACIAYGAAMSHEHFVAIDIAAA"
     gene            50194..50583
                     /locus_tag="PK198_00290"
     CDS             50194..50583
                     /locus_tag="PK198_00290"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRF37197.1"
                     /translation="MTSLRLDMLRFKLDENFSPSLTELFKAHNYDAESVLDEQLSGAP
                     DSDIYEVCRQENRCLITFDLDFANILRFPAESTQGIVVIRTNQIITLEVMKQMLEILL
                     QLFESSSPTGCLWILEPHQLRIRKPKE"
     gene            50714..>51117
                     /locus_tag="PK198_00295"
     CDS             50714..>51117
                     /locus_tag="PK198_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37198.1"
                     /translation="MKYLLLLSLLPLLLSAQPATRKQQPPFQEFRLDNGLRVMVVQNK
                     KLPDLHFHFFTQLPVAPEENLVGYEELTAVMQAIGPASKAMWDEQVAHWGALFTAGPD
                     GIMAVCQARYREQMMAFMADMVLRPAFDPVDF"
     assembly_gap    51118..51169
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51170..>51484
                     /locus_tag="PK198_00300"
     CDS             <51170..>51484
                     /locus_tag="PK198_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF37199.1"
                     /translation="PATVAANVAAVLAFGKTHPYGELPTPQTIRNIELKHCRTYYHAF
                     YRPEMSCLVIMGDVRLPDARKLVESHFSNWQQGGILTQSFELPPLPRSTQIAYVALPD
                     ADT"
     assembly_gap    51485..51504
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51505..52721
                     /locus_tag="PK198_00305"
     CDS             <51505..52721
                     /locus_tag="PK198_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF37200.1"
                     /translation="YPVQLRPGDPDFWKTLLLNTVLLQRLNDKAGKFATDFQFGITPD
                     REMALFVAAAKVPSRHSDSLVGLLLNEIEVLRREPLSETEMERARQLLHDTWKWTWGR
                     PFRVSRFAIDVARYRLPADFFHRWREQISKTTPQQLHAAARKYLPEEQAHILVAGQRE
                     DAAALTRFDGDGRVVFYDVYGTPIDDNNLLMPEGLDAKGIIEGYIQAVGGQERLASIS
                     DLTLHMSAQVQGQEVKMTIQKKGPGKALVVSSVQGNVLTKIAFDGAQASVYAMGEKQA
                     LDDQATASIQRQAWLFPEIEYLRSGSLSFGGLEDINGRKAWRVDVVTQKGYACSEYYD
                     VETALKLRSVTIEAGMRVVNNYSDYREQQGILLPFEILTTFAGEQQPPMLMRVSEAKL
                     NTGLSDSIFFIR"
     gene            52743..53219
                     /locus_tag="PK198_00310"
     CDS             52743..53219
                     /locus_tag="PK198_00310"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF37201.1"
                     /translation="MQEDDLILDEPLFEDQEWKPAHASKGQRFVNYLLDQVAMTGMMS
                     AFFIFSGLVEVFEDSKLGNWIIGAISALIYYTLMESFLQGKSLGKYVTKTRVVTHEGY
                     QPELSTILVRSLSRVVPFDALSFLFKEPGGWHDEWSKTYVVEDRCLRVNRSPVETV"
     gene            53226..53717
                     /locus_tag="PK198_00315"
     CDS             53226..53717
                     /locus_tag="PK198_00315"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRF37202.1"
                     /translation="MIQVFVTGGTFDKEYNYINGALFFKDTHLPEMFSRGRCTVEVDV
                     KTLMMVDSLEMTDADRDIIIHNCRRCTHQRIILTHGTDRMVETARHLAQAGIEDKTIV
                     LTGAMIPYAFGTSSDGFFNLGSALAFAQTLPPGVYVVMNGRYFEWDHVRKNLNTGNFE
                     EVS"
     gene            complement(53732..>54694)
                     /locus_tag="PK198_00320"
     CDS             complement(53732..>54694)
                     /locus_tag="PK198_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37203.1"
                     /translation="SKVSIFSDLNNLNDITLNPRHATLTGYTQPELENTFQEWLQYTL
                     EEMPGVTTEELLAQIKTWYDGYTWNGRDHVYNPFSVLNFFASGSFQDYWFKTGTPTFL
                     IQKIKESGYFNLNDLKVSGLIFESYSLDNLETRSLLFQTGYLTIKNIDHIRGIYTLDY
                     PNREVEQALTGHLLSVLLHRAAPETVVPVQQLEEAFFANDIARVVTVINSMLKGVPSH
                     LLDEKGEHFYHALVHLHFRYLGLFIQSEVHTSDGRMDAVVQTPTHIYLLEFKIDQSAE
                     AALSQIRRKDYAAALRLENKPVIGIGINFDSVKKAVSDWAEEEV"
     assembly_gap    54695..54728
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<54729..>55068)
                     /locus_tag="PK198_00325"
     CDS             complement(<54729..>55068)
                     /locus_tag="PK198_00325"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37204.1"
                     /translation="QWNWTKVHPVVHIQFNEIGYNTGGLESALHKTLEEQAAMYGLSL
                     KEATYDQKFRELIRELSEKKGKVVLLIDEYDKPLIDYLEKDQLPTAFEHQRILKSFYS
                     IIKSADPYLE"
     assembly_gap    55069..55143
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(55380..55913)
                     /locus_tag="PK198_00330"
     CDS             complement(55380..55913)
                     /locus_tag="PK198_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HRF37205.1"
                     /translation="MKRILIDMDEVIADVYPKFLDIFEREFGRRPDRMEFWGKKIYEL
                     PGADRVRGYLHDKGFFSDLPVMPGSQEVIRELMDHYEVFINSAAMEFRHSLTEKYDWL
                     EEHFSFIPWKNVIFCGDKRALRGDYMIDDHVKNLRSFEGTPLLFTASHNALETDFIRV
                     NNWEEVRAFFRGQREGA"
     gene            complement(56108..>56868)
                     /locus_tag="PK198_00335"
     CDS             complement(56108..>56868)
                     /locus_tag="PK198_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRF37206.1"
                     /translation="IETSEHNRLSEILNILFDFDERILVKIIPDMYDILLGTVKMNHL
                     YGAVLIEIKRGLMARWQRLIKRLIDVAASSVFLLLLSPLYLYIALRVRWSSPGPIFFK
                     QERVGINGKPFLIFKFRSMSLDAEAEGPRLSHDGDPRCTPWGAVMRKWRLDELPQFFN
                     VLIGDMSLVGPRPERQYYIDLILQKAPHYKHLLKVRPGITSWGMVKYGYASNVDQMVQ
                     RMKFDILYIENMSLALDFKIMFYTLLVLLQGKGK"
     assembly_gap    56869..56974
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<56975..57539)
                     /locus_tag="PK198_00340"
     CDS             complement(<56975..57539)
                     /locus_tag="PK198_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRF37207.1"
                     /translation="MTRKRQRHTLMYQAADFVTAMLSWACFFMYRKHLEGASLGTATL
                     NDPNFLYGILIIPLGWLLFYGIFDQYNDIYRMSRLATLTRTLFLSFFGVLFLFFTLIL
                     DDFVTNYTTYYNSFFTLFLLHFSLTGTVRMILLTRASRRLKSGEITYNTLLIGGNQNA
                     LDLFEDITGREKGLGYKFIGFIDTNGKS"
     gene            complement(57665..58549)
                     /locus_tag="PK198_00345"
     CDS             complement(57665..58549)
                     /locus_tag="PK198_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37208.1"
                     /translation="MRFFLQTSLLIWGLATLTGCDLINPEEPIPGYVSIEPFQITTTA
                     NQGSASSKISEVWVSAGNDFLGAYRFGTAFPVLGTGPQPLTLQAGIKDNGIGELPEIY
                     PFYAPVTVTVNLGANETQIIRPMTSYLPETKFAFIENFEGPQHIFRTVRTGTTAQAVQ
                     RSAEMPFEGEASGVFVLDSMNTVAEVVALPLLSGLNSSSPYVYLEMNYKSDVDVLVGL
                     VGFETGGPIAGTTLYESGFRARADWNKIYFNLSQTIFNSRFPQYQIVLQAAIPVQNGV
                     PEKKVAKVWIDNVKLVHF"
     gene            complement(58577..58705)
                     /locus_tag="PK198_00350"
     CDS             complement(58577..58705)
                     /locus_tag="PK198_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37209.1"
                     /translation="MSIEDGYDALRLAWRIIADMERRQAAAGLNIDKSGGDIRITS"
     gene            complement(58702..59601)
                     /locus_tag="PK198_00355"
     CDS             complement(58702..59601)
                     /locus_tag="PK198_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF37210.1"
                     /translation="MLKIGVFGAGYLGKIHLKCIGLASDVWELAGFYDPDDATALQAQ
                     ETYGAHRFETIEALLDAVDVVDIVTPTPTHFALAKAAIAKGRHIFIEKPLTHTLAEAE
                     ELVRLSQAAGLKVQVGHVERFNPALLALGDVPLHPMFIEAHRLAQFNPRGADVSVVLD
                     LMIHDIDIILSLVQAPVTAVSAGGVAVVSDTPDIANARIEFGNGCVANLTASRISLKQ
                     MRKVRLFQRDAYISLDLLEKQAQVIQLRDKDDASPAQGIPVMEMPTATGTKVVHIQTP
                     PIEPVNAISKVLQRASCNRAAPK"
     gene            complement(59756..60055)
                     /locus_tag="PK198_00360"
     CDS             complement(59756..60055)
                     /locus_tag="PK198_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37211.1"
                     /translation="MQIALIFSLMALFAAMVVLNFYFRIRVLRTYQRLARQNVRFGAA
                     HFFNARRLEEEILPLYPEAKTDILNFVKYIRFSVRMASVLIALITLLGGVLMYYR"
     gene            complement(60523..>61579)
                     /locus_tag="PK198_00365"
     CDS             complement(60523..>61579)
                     /locus_tag="PK198_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF37212.1"
                     /translation="DTLFIGDVGRPDLAQKAGSVTKEDLAGWLYDSLRNKIMTLADDV
                     IVYPAHGAGSACGKNMSSDTWDTLGHQKETNYALRADMTKDEFIAEVTAGLAPPPQYF
                     AKNAMLNKKGYGSFDAVLKRGAVPLSPAEIELLTESEGALILDTRHEDVFREGFIPGS
                     IFIGIDGAIAPWVGALIVDLHQPIVLLTPEGREEEVVTRLARVGYDNTLGYLKGGIEA
                     WTATGRAVDTIESVTADELALRLKVGVVDNYLDVRKPSEYLSHHALSAQNFPLDYINN
                     NMYKLDARQKYYVQCLGGYRSMIMASILKARGFQNVVDVYGGWRAVEESQVPKTDFVC
                     PTTIPQEIIDDAMAAAY"
     assembly_gap    61580..61594
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<61595..>61905)
                     /locus_tag="PK198_00370"
     CDS             complement(<61595..>61905)
                     /locus_tag="PK198_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF37213.1"
                     /translation="VIIDPLRETQPYMEKLEKEGATLKYIFETHFHADFVSGHLDLAK
                     KTGAAIVYGPGAATEFDKHEAQDGEELKVGRLTFKILHTPGHTPESTTFLLKDENGKD
                     Y"
     assembly_gap    61906..61926
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            62363..63070
                     /locus_tag="PK198_00375"
     CDS             62363..63070
                     /locus_tag="PK198_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37214.1"
                     /translation="MRNDKHTNLSGIMLHITIPSTMKKLLFSACICLSAAGMLCAQKM
                     SISNPTPFELSRELTIAIAPASIRYMDLNTDALLPKERYNGEAVAAFFDRRLEALLLQ
                     QQLPLCAGPDSLFIGGQQRLLAQGYALVRALQDEKTASELKSNFADAGCRLIMVADIR
                     VKVGDDAEWEGANPLYISLVVHPQTHTTRIKTALIDTASASVLWKNELETKGIPDVAK
                     PRWAEVFETIFSNIKTK"
     gene            63073..63648
                     /locus_tag="PK198_00380"
     CDS             63073..63648
                     /locus_tag="PK198_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37215.1"
                     /translation="MKNNLVQLLRYSVVLFGLALVGSCIPAAKPLFLDSSFHSVKIDT
                     IAVLPVADMRTDKSLEISKVDQWRVRPFRNALTRARYASVYIENRALVDNIAEEDLEE
                     LDPSWIKRLNPGQHRWVLLPIIYGSHKQVDIGKKVQLEIAAALFDVQTGKRIWFHKDA
                     PKCSGGLLMPGVMQDCIQTSYWALAMNLPKQ"
     gene            63796..64635
                     /locus_tag="PK198_00385"
     CDS             63796..64635
                     /locus_tag="PK198_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37216.1"
                     /translation="MKNSMYFLFTTLFLVFTLSANALPNCNSSNNADTPEWSRGIPEL
                     WQSDTLPSLKPENGEWGFGIRAEGLQGLIWNNNFDSLSLQLRYALRPSAVFRVDAGIA
                     LYKEKTSLKDVFSNGGHQYNEDAETLSSFSISPAMEWHFNGTRRLDPYLGLALPLVFV
                     GKRHITEIDETINSNGTFFKREILRELPGGLGFGINGIAGINYFVSNRLALGLEYSIG
                     ASLVNVSGKIKMKTTTRSKPSPTGSETITITESGDDEISQRDIFFGNKGVAGINLIFY
                     LGR"
     gene            64681..>65242
                     /locus_tag="PK198_00390"
     CDS             64681..>65242
                     /locus_tag="PK198_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37217.1"
                     /translation="MKNHPLSLSARVLFAFAFSLFSMVLYAQSTISCSQSTSANLTNS
                     SPVNTFLLSANNNDRVRIRAYSFVAGLNLRIEVRSPDNSVSLGVFFANNGLVDIQELT
                     LGAGNGQYRILVSDAESNTTGTYNISVQGLNKTTCASLVSCSASSSGSYFYSAAIEGK
                     YLVASAGDVLRLRYFFVDPNPEWRLEV"
     assembly_gap    65243..65342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <65343..>66771
                     /locus_tag="PK198_00395"
     CDS             <65343..>66771
                     /locus_tag="PK198_00395"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HRF37218.1"
                     /translation="LRLRYFFVDPNPEWRLEVRRPDGTLHALLAQNGSGLIDTTMLLP
                     AAGNWFFYLYDEDGDEANTTRTYHYSFRLLKTSCATALNCGGSVSGSILYRPEIDLHY
                     FYAEPCDTARLLFSAVDPSFYEAISLYAPNGALIGQYEAVNSSDPAILQVPLSAYGYG
                     YYLVAVYEKEGNSLTFTNYTLSLSCLRSSLNVAPASMNINALGGNQTLNVSGPCPSWS
                     IAGSANWATANPNTGSGSGPVVVTFGPNQTTASRSAQLLLVGCCDSTFVNLFQSGATL
                     SVSPSSINAGIAGGAYSVNVTSTCIQWTVSSSQDWATPSVSAGSGSQSLSIMCSANTT
                     GLGRNAVITFSGCGINVTINVVQPAATLACTPGNINPTGAGGDYNVTLSTNCPLNWIS
                     SVAPSAGIWIEVLPVFGNGSGNVVIRVSPNDAPIARSGTVEFSACDLTRSISVVQSGA
                     TLNATPFNFTISGSAAGSVVCNLSGT"
     assembly_gap    66772..66792
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <66793..67639
                     /locus_tag="PK198_00400"
     CDS             <66793..67639
                     /locus_tag="PK198_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRF37219.1"
                     /translation="ITGAPDWLTVSLSGNALPATATLLYTANNSGVTRTATLTLSGCG
                     ITRSITVTQPGVLIISGSTSYCQGSSTQLCAPTADAYLWSNGATTRCITVSTPGLYNV
                     VITQGGTQQSGSVTILELPAPFPPFIVQFDANVLLAVGDASSWRWYLNGQLIAGANGN
                     SLPFTAGGSYTVTAVGANGCESQPSVPFLITSVHEPAKAGRLKVTPNPCSEWFEAEIP
                     DRVISARCLVFDAYGRLCFDKKEVTGQVVRVPRNGLPAGWYLIRIIDNRGTVSATGKV
                     LFVDY"
     gene            67736..68350
                     /locus_tag="PK198_00405"
     CDS             67736..68350
                     /locus_tag="PK198_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF37220.1"
                     /translation="MSESYFRQTHQSRRGMEKQDLSFTDEQILEFVKNRKMHQRVFQW
                     ADSKVLPGIQTMMKHRGNPSENDIRTFFLTALTDFFHILEAPEYQLRENSNLNGLLFT
                     ICERMWIRECKIRGRFSGGDMPDPPASSSSDHDLIHREKLAMVSAAVSRLEQPYRGVL
                     VLHEYGELSHAEIALAAGYASPDVSKEIKSRAIKKLREIYKNIS"
     gene            68356..>69095
                     /locus_tag="PK198_00410"
     CDS             68356..>69095
                     /locus_tag="PK198_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37221.1"
                     /translation="MDSQNKDFDLLCDDYLHGAMSEADKSEFEALLQSHPEHAAQWQE
                     FCILTQGVREYGRRQMLETWYNEAHGKADDEKPAAGKQPTLMDIWKAQQKAAAGESGA
                     SSGKRALPIFRYISAAAIMVIVFFVGWRLGNNTDPETGRGDDVILLRQMELPVVSIQT
                     RQEYRPDTNAEIWLVRVEKNLRPESVAILSGRQLTLVLPTSEAPDDIRLRRLDGRVYI
                     GMGGGWYPLRTDEQREQILLPVEDVSILS"
     assembly_gap    69096..69133
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <69134..69990
                     /locus_tag="PK198_00415"
     CDS             <69134..69990
                     /locus_tag="PK198_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF37222.1"
                     /translation="GLLMFLAIFMSFLVPAGAQDDSGVLPEDEREMKSVRQFRATERS
                     GQYPSFFLFDKLAISPEKFFCNGFPLDQGRCASCAGCAIAQAYSIHYAFWTKQKNRKP
                     SWLKFSGSFVYNQIKQPGDCRQGARISSGLKLLTAKGICPADTFPHSPYTCGDLPQKQ
                     HWKVAAGYKIAGFEKVTTPDMKAKPDSVIINALKNAIVYSNPVIACIQIPKSLAELPE
                     GECRWQAQGKESTYWHAVVITGYDEHHFQILNSKGCRWGCNGLALVPHAQLASMLEWS
                     FVMTYLPR"
     gene            70002..>70229
                     /locus_tag="PK198_00420"
     CDS             70002..>70229
                     /locus_tag="PK198_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37223.1"
                     /translation="MITQICRPLMFALCLATIQAAAQPNCYALLLKSDTLMGRSKWKE
                     AAPLLEEAYRVCMEEHGFESDESAWIHNNLYI"
     assembly_gap    70230..70436
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            70491..>70692
                     /locus_tag="PK198_00425"
     CDS             70491..>70692
                     /locus_tag="PK198_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37224.1"
                     /translation="MGIAYGSIGYFDKSIEATTAALELEGKINNKLSSIYANRLHNIS
                     TIYLLKGEQERAIPLLHQSIALF"
     assembly_gap    70693..70706
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            70888..>71871
                     /locus_tag="PK198_00430"
     CDS             70888..>71871
                     /locus_tag="PK198_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37225.1"
                     /translation="MLVLTGQFEEVEKLYGRHLNELTSSDGDNLTKALLMEKLASAYL
                     FSNRPEKAIPLIEKAIEIVQKNTGESSYYSLGLKSVYAQLLFLSGRTEEAIAVGEPNL
                     LNVISALGDLAPLSAGQEAVLAKCYASTAQLDKALTSARNSLRRADAIFGEQHPQYLA
                     YLALFVNTLYLSGETAEADAHFVRLTALIQSNLLRFYSFFAPKEQLNYLALSQTNLEF
                     IEQYVYLNSNRNPELATTLFNSNSSLKGALLDDSKARRAWLLQNSDSATVVLQKEWTN
                     IGQALAKQYALPIDQRTIKDAQILDSLQNRFQKLEQQLSDASAAFRKAFAGK"
     assembly_gap    71872..71890
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <71891..>72241
                     /locus_tag="PK198_00435"
     CDS             <71891..>72241
                     /locus_tag="PK198_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37226.1"
                     /translation="WNTIKNKLRPNEVLIDFFSFRLYDASSDTQADSVLYCALVGRRD
                     WAAPRLVPLFEEKQLLQRLALADEPLGSAQNVISSIYDRHLYQILWQPLEPYLQGAST
                     LWLCPEGALHKIAF"
     assembly_gap    72242..72263
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <72264..>73066
                     /locus_tag="PK198_00440"
     CDS             <72264..>73066
                     /locus_tag="PK198_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF37227.1"
                     /translation="NGRFAGQKYRLQMLGSPRDILHYELLELDRINDALVYGAIQYDV
                     SEELSDCMESKIPKNDARQLASALRNSGCGTRLPYLAGTDAESDFINSLLKNNRIETV
                     SQKGCEASEDYFKYACRNKKGGFSLIHLPTHGLFCRPPVLAVPELNKNSDIAPNPLLY
                     SSLALAAYNRKASNRPLPPHQEDGELTAYEVSQLFLPQTQLVVLSACVSGLGETQSTE
                     GVFGLQRAFRMAGARHVLVSLWQVDDQATNQLMQLFYHFWLVKKMDAAQ"
     assembly_gap    73067..73083
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            73206..>73535
                     /locus_tag="PK198_00445"
     CDS             73206..>73535
                     /locus_tag="PK198_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37228.1"
                     /translation="MSKFLVLLSAFALYSCAGAAQMAQPAGATRTLIAFGSCAQEAKP
                     QPILQQVADLRPDFFVYLGDNIYGDTYDMNVLRAKYAQLAAKPEYQALRRATTVLATW
                     DDHDFGWN"
     assembly_gap    73536..73609
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <73610..74272
                     /locus_tag="PK198_00450"
     CDS             <73610..74272
                     /locus_tag="PK198_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRF37229.1"
                     /translation="PADSDRRKREGIYTSYLYHYGGKKLQIILLDTRTFRDNLRNYRG
                     ELHNQSRYFYPLDYFPHQTSDSTLLGEQQWRWLETELRRPADLRIIGSSTQFGIEFNG
                     YEAWANFPHEQQRMMDLIQKTRAEGVLFISGDVHYAEISKIQPPGAYPIYDVTSSGIT
                     STWHFPTPNAHRIEGPVMENHFGLLSIDWKPADPLIRMEVIDASGNSRIEYTVPLSQL
                     RF"
     gene            complement(74437..>76523)
                     /locus_tag="PK198_00455"
     CDS             complement(74437..>76523)
                     /locus_tag="PK198_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRF37230.1"
                     /translation="EASKIRRSILARNPDAIVNGRLQGYGDYATPEQNFPVSRPPHKW
                     WELCMTINNNWGFQHRDTAWKSPYQIITIFADAIANGGNLLLDIGPMEDGTIPPQQVQ
                     VLEALGDWNRKHGRAVFGTVAGLPQGHFYGPSTLSKDSTRLYLFLTGNPGQDIILKGL
                     ASDIERISVVGSETPLDWKIVGKISWSSVPGLVYAKVPAHLHDKYLTVLEVQLKQPLK
                     LYRGEGGLTDAPSETHEPEAPYIWPSDPAVSEQLHRWQDWKFGVLMHWGPYSQWGVVE
                     SWSLCPEDEPWCERRGPFADDYAAYTREYEQIGKVFNPQQFNPQRWAKACRRAGMKYL
                     VFTTKHHDGFCMYDSRYTDYKITGAGSIFASDPRSNVVREVFDAFRRQNMGIGAYFSK
                     PDWHSDDYWWPYFPVFDRNVNYDPTKYPERWKRFQEFTYNQIEELMSDYGKLDLLWLD
                     GGWVRPAGSLTAETRPWLGKNQWTQDVNMPAIAAMARRKQPGLLMVDRTVHGDFENYR
                     TPEQQIPADVPDYPWESCITMGDSWYHTGPGERYKSSTWAIHTLAKIVAKGGNFLLGV
                     GPDKTGELPPAVYERLDAIGRWMDVNSAAIYNTRPLAPYQSDKFCFTQSKDGSTRYAI
                     YLRAEDEAMPERLELPADFVGAAQQVTLLGHAGKLKVQELNGAKMVVLPKMFRQSMAD
                     VPALVISWSRGG"
     assembly_gap    76524..76563
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<76564..77160)
                     /locus_tag="PK198_00460"
     CDS             complement(<76564..77160)
                     /locus_tag="PK198_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRF37231.1"
                     /translation="MRHILFALLLAAALPASAQQPSDDRKMQWFADAKLGIFIHAGIY
                     SVKGVDESWSFYNKKISHAEYMQQLNGFTLSKYDPAAWADLIQQSGARYAVITTKHHD
                     GVAMYDTRMNRLSSLHATPARRDMIAPLFDELRKRNIKTGAYYSLIDWTHPDYPGFLK
                     DSNRYEIKNDPARWNRFRRFFQGQIDEIGERFQPDLWWF"
     gene            complement(77200..77856)
                     /locus_tag="PK198_00465"
     CDS             complement(77200..77856)
                     /locus_tag="PK198_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRF37232.1"
                     /translation="MRALFLFTLFLIVAGPLLGQELRIVDRPIVWDSTRARLSLEYLE
                     KRHGIVQPEPVIAPRMVVVHWTAIPTLEGSFRAFDPVELRARPELVNASTLNVSVPYL
                     VDRDGTVYRLMPDSLFGRHCIGLNYMSIGIENVGDGRKHKLTKAQLEANVLLIRQLFG
                     RYPIEYLIGHHEYLRFKTTDLWKETDPNYFTNKVDPGKKFMRQLRKRLKDLKPLGPPR
                     "
     gene            77905..>78795
                     /locus_tag="PK198_00470"
     CDS             77905..>78795
                     /locus_tag="PK198_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37233.1"
                     /translation="MSFLKRLFKSTDSQPAVPEVRFGRYSDSYKETENYKAWDQSVEA
                     FEEKQYLDSLRAFLRYLRDEHQDNVHWQDHGDRIEFEICQGSKLISGLADALHFKAES
                     KVAKAKDLDVAFMRRLVEQNFDNLYSRYALDPDNNITVVFDTFTLDGSPYKIYHALKE
                     MATHADKQDDLLLDEFDSLEPISSPALMPVPDAEKQTKYDYICREIDAALRVMEDGRL
                     DKNQYPGGVGYLLLCLVYKLDYLTQPQGFMMETLERAHRLYFTNDGKTTAQKNLALAK
                     ELQTLRQRSQTLFYKEMYRAS"
     assembly_gap    78796..78827
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <78828..79242
                     /locus_tag="PK198_00475"
     CDS             <78828..79242
                     /locus_tag="PK198_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37234.1"
                     /translation="VNQRHIAGFIESELPNMDWYCENGHTEVALSVPGYIAGYCLFNY
                     APPRPVKDLLHLYFQITEAGFFKHLGFPAAMFEPETGVFHKKAIRQAIEGIVEKNRER
                     FHLQPDLSLLQWNDLPTFARSLLAMLARMEVETRF"
     gene            complement(79344..>79530)
                     /locus_tag="PK198_00480"
     CDS             complement(79344..>79530)
                     /locus_tag="PK198_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF37235.1"
                     /translation="EDKLPTAIFKRIHRSFIVNLNKVHAIVGNMVEVMEKGQAKHLPI
                     GKNYRDELLDLINQNRI"
     assembly_gap    79531..79565
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<79566..80088)
                     /locus_tag="PK198_00485"
     CDS             complement(<79566..80088)
                     /locus_tag="PK198_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF37236.1"
                     /translation="MINVIIVDDEPLAQDVLETYIGRVPGLHLVQKCNNAFEANEALK
                     NHEIDLMFLDIQMPQLTGIDFLKTLSDPPLVVFTTAYPNFAVEGFELNALDYLLKPIS
                     LERFMKAVNKASEQLAPKRRESPATALTEAAHDPAHEFIFVKADKKLVKINFDDVVYI
                     EGLKDYVIIKLDAS"
     gene            complement(80124..81179)
                     /locus_tag="PK198_00490"
     CDS             complement(80124..81179)
                     /locus_tag="PK198_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF37237.1"
                     /translation="MQNPLPAHWRASFNRVLYRTSSRHILFWLFVAILLTLLEGAHNG
                     FWFAFTNESINVFFYGILVYFNFNYLIPNYLTKNKFLTYAALLILAAVIITPIKAIVF
                     YVKFAAHPLTRGELIENLNWYFLVHFFVAGASSIVKIVADWAKQLRERQELENQTMQS
                     ELRFLKSQINPHFLFNTLNNLYALTLKKSDDAPEIVIKLSEMMRYMLYDCNEKQVPLS
                     KEISYIHNYLDLEKLRQSKNADIRFEVSGMVADQQIAPLMFIPFLENSFKHGLTNALA
                     QGFVHIYLQVEGRHISFFIENTKPDTKPVQDRKRSGGIGLVNVRRRLQLLYPGKYELS
                     VKDNPNTYAVSLEMDLN"
     gene            81403..81948
                     /gene="folE"
                     /locus_tag="PK198_00495"
     CDS             81403..81948
                     /gene="folE"
                     /locus_tag="PK198_00495"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRF37238.1"
                     /translation="MNAYTQILGQLNDDAQREGLLKTPERAAKAMQYLTKGYEEDGEA
                     ILRSAMFKESYSEMVIVKDIELYSLCEHHLLPFFGKAHIAYIPDGYIVGLSKIPRIVE
                     VYARRLQVQERLTDEILHCIQNTLKPMGVAIVIEAQHMCMMMRGVQKQSSLTTTSAFT
                     GAFRRVETRTEFLNLIGNRLH"
     gene            81962..83377
                     /locus_tag="PK198_00500"
     CDS             81962..83377
                     /locus_tag="PK198_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF37239.1"
                     /translation="MIFSIQPYEREIFRIISQTAARLGYPAYVVGGYVRDRLLARPSK
                     DMDIVCVGSGIQLAEEVAGQLRPIPRVAVYQRFGTAMLRHGDLEIEFVGARKESYRHD
                     SRKPSVEEGSLEDDQNRRDFTINALAVSLNEVDFGTIIDPFNGLQHLELKSIQTPLEP
                     GRTFSDDPLRMMRAIRFATQLDFNIEEQTLQAIGRYKDRIKIVSMERIAIELNKIIDS
                     KVPSVGFKLLFDTGLLHLIFPEMTALHGVEKRQGKSHKDNFYHTLEVLDNLARSTDSL
                     WLRWAAVLHDIAKPPTKRFEPEHGWTFHGHEVLGANMVPRIFGRLKLPLDQKMKYVQK
                     LVLLHLRPISLTKENITDSAIRRLLVDAGDDLEDLMLLCRADITSKNPAKVQRYLENY
                     ELVQQRLAEVEAKDHLRNWQPPITGEIIMETFGLEPSREVGAIKNAVREAILDGIVAN
                     DYEAAFEYMLERGKEMGLERV"
     gene            83803..85830
                     /locus_tag="PK198_00505"
     CDS             83803..85830
                     /locus_tag="PK198_00505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF37240.1"
                     /translation="MRISSYFVLTILLTIAAVANVSAQKKTFTPEALWKLGRVSLQDV
                     SPDGATALYTVTYYDMAANKGNTDIYAVATDGSRPARKLTAFPGSENNARFRPDGQRI
                     GFLAGGILWEMNPDGSDQMQVGSSPIGGFCYSPKGDYIAFIQDVKYRKSVQDIYPDLP
                     HAKGRIIDQLMYRHWNYWDDYSDPNVFIQAYGNGQLKGEAMNIVGQAIEAPVEPFDGI
                     EEVVWHPGGRKLAYSAKRQTGTEYAKGTNTDVFLYDLDAKTTTNLTEGMPGYDKHHAF
                     SPDGRYLAWLSMATPGYEADKNRLFVLDFQTGKKEDLTEKWDESIDAMQWAPDGQSLY
                     FGAGVNATKQLFQIDMAKKRVRQVTKGQWDVGAFVAGKRHIIAARNDMNTPAELFRID
                     PATGDMQPLTFTNRDMLAQFDDISVRKRMVKTTDKKDMHVWVAYPPNFDPNKKYPALL
                     LCQGGPQSLVSQFFSYRWNVKLMASQGYIVVAPCRRGMPGFGMEWNRQISGDWGGQAM
                     QDLLSAIDDVKKEPYVDAERLGAVGASYGGFSVYWLAGNHQKRFKTFISHCGTFNFES
                     WYGATEEVFFANFDLGGAYWQEPKPVSYDKFSPHLFVKNWDTPILVIHDEKDFRVPIG
                     EGLQAFQAAQLRGIPSRFLYLPEEGHWVLTPQTSLLWQRVYFEWLEQWLKG"
     gene            complement(85832..86479)
                     /locus_tag="PK198_00510"
     CDS             complement(85832..86479)
                     /locus_tag="PK198_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HRF37241.1"
                     /translation="MAKKQKFYVVWKGHQPGVYGSWNECQAQTMGFAKALYKSFDSEA
                     EARKAFLDGPARHIGGSPAGAKSKSSPKPAGMPSNASVIWDSLSVDAACSGNPGVMEY
                     QGVNTRTGEQLFYKKFPLGTNNIGEFLAIVHALALTKKQGLNTPIYSDSQTAMGWVRK
                     KHCKTQLDHNAQTEELYEVIRRAESWLHQHSWSNPLLKWETDRWGEIPADFGRKG"
     gene            complement(86579..87136)
                     /locus_tag="PK198_00515"
     CDS             complement(86579..87136)
                     /locus_tag="PK198_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF37242.1"
                     /translation="MVNYEIKNGIILLAEPFMLDPNFRRATVLLCEHSPEGSVGFVIN
                     RPTEYRIDELLEDFPEFNSIVYQGGPVEMNTIHYLHNLGDLLEDCVKVTEGVYWGGDF
                     DKLKFLINSGLVTPDNIRFFVGYSGWDEGQLMDEMATGSWVTADMHPNYVFKTQADEL
                     WQQVMHNKGDVYSVIATMPDTANWN"
     gene            complement(87270..>88044)
                     /locus_tag="PK198_00520"
     CDS             complement(87270..>88044)
                     /locus_tag="PK198_00520"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRF37243.1"
                     /translation="NEVLGYRLTDVMFEGTSEDLRQTKITQPALFVHGVVKALILGDD
                     FQPDAVAGHSLGEFSALTAAGALSFADGLRLVQQRAFAMQKACEAVPGTMAAVLMLSD
                     DAVEEICASVSDIVVPANYNCPGQLVISGSQAGVEAAAEKCKEAGGKVTMLAVGGAFH
                     SPLMQPAQEELEAAIAATPFQAPRCPVYQNVNALPATDPEVIKANLINQLTAPVRWTQ
                     TMQHFIRHGFTEMIESGGTGKVLQGMMKRLDRAFATSAL"
     assembly_gap    88045..88271
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(88300..88956)
                     /locus_tag="PK198_00525"
     CDS             complement(88300..88956)
                     /locus_tag="PK198_00525"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37244.1"
                     /translation="MTQGISFIISDLLAKAQLQEAAATIQEFLKDKNRPDLDDQITMV
                     IMQLNKLANDYYVLQILSEEDARKTHNELGFSLLNITKEVKALEKEWQQQQANMRDPA
                     IESLVYAHMHAMLEKDIDGIMKTFHPDMPGREQTQETYLQIFQQIDALPEVRSIRLLS
                     KTDEMAGAEVVQFTRNAIEGVPFRENETTQIYLFRKAGGLWKIYNTIVKKIEYFDFTE
                     "
     gene            complement(88972..>89570)
                     /locus_tag="PK198_00530"
     CDS             complement(88972..>89570)
                     /locus_tag="PK198_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRF37245.1"
                     /translation="TSKPVTVKTRLGWDEKSINILDVAERLQDAGIQALTIHARTRKQ
                     MYKGEADWTWIAKVKDNPRMHIPIFGNGDVDTPQRALEYRNRYGADGIMIGRGAIGSP
                     WVFQQIKHYFATGELLPAPDIAERVEAVRQHLRDSIAWKGVKLGVLEMRRHYSPYFHG
                     FHGIKPFRTRLVSISDPAELMAVLDEIEHSYAGQEILV"
     assembly_gap    89571..89656
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<89657..>89889)
                     /locus_tag="PK198_00535"
     CDS             complement(<89657..>89889)
                     /locus_tag="PK198_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRF37246.1"
                     /translation="CKRYGADMMYTEFISADGLVYESDKSFQKLEIFDYERPIGIQYF
                     GGQLDSMIRAAHFVEAANPDVIDINFGCPVAKV"
BASE COUNT        20403 a        23902 c        22591 g        20373 t
ORIGIN      
        1 aatgaggtgt ctttgaccac tttttgcccc cagagataca gcggcatcag caaaaaaaca
       61 atggcgacag cgcgcaaaac agtgtttgtc atattcggcg ttttgataca aagacccttc
      121 cggcggggga aaggtttgaa tttttgttca aaattaacaa cttttaggaa aagtcgggcg
      181 gcccttatgc tccgaccgcc cgacttcgcg ctgctacctg cgttcccgct tcaccggtat
      241 ggctacccgg ttgagcttga tgattctgag cttttccagc agcagcatga taacgtaagt
      301 cacgtctatt tcgtgccacc gcacgccgcc gaaattgggg cgcccgccat gtttgtggtg
      361 gttgttgtgg taagcctcgc ccatcatcag ccagtcaaaa tggaatagat tcttggaggt
      421 gtcgtccact ttgaaattgg tgtagccgta tttgtgcgca aaccagttga taatcatgcc
      481 atgaatgggc gccatgagaa aagccaccgg cagcagcaac cattgccacc aggccgtagc
      541 cagaaaataa aacagcacca cataggccgc accccagccg aggcgcgatc cccaggaact
      601 ggcaaacttg tcgaagccct tccattgggg cacatttttc gtgaattttt cgtctatctg
      661 cactttgtcg cgcacgatgg cggcgtattt tttccaggtg cgccacatca tggctatggc
      721 gttggcatcg tatttcggcg agtgcgggtc ttgctcggta tcggcaaaag cgtggtgcat
      781 cctgtgcatg acaccatacg cataagcgct gaggtaattg gaaccctgtg tgacccaggt
      841 gaggatgaag cagatttttt ccgtcacttt cgacatttca aagctgcgat gagcggcgta
      901 tcggtggagg aaaaagcttt gaaaaaacag ccccgaatac cacagaatga tgattagtac
      961 gataactccc atgttgttga aaaatttaaa agtggttgaa aaatggtctt tcgcttaaaa
     1021 gttgtcaatc ggggcggcaa gttagaacct gatgggccat gccgccaaca atcggggaga
     1081 gtttttttgg tcgccataca cccatgcagg cacaatgatt taattagcaa tcagttaaac
     1141 cctgactgcc gggacatcgt ttttctgtta aaacttcttt cttgttgagc aatcgctcaa
     1201 aaagcgctaa ttttgtccac acttcgagaa agtgagtgaa tgtcgctccc gttcaattgg
     1261 cacaactccc gcccatgcaa aaattcgatt atccaatcct ctactactct gttaacgaaa
     1321 ccactgtact gggcctgctg ccgggcacgg ggcttcaggt ggtggaaaaa gacctcaaaa
     1381 ccgtcaaagc ggtactgggc gactacctgc tgaaacagta caaaaaaagc ggactgctgc
     1441 cggggcggca cctcagcaat gccaaaatca cagctttcga cgtcaatgtg cggccttcct
     1501 acaacgacaa ttccgttcat taccccatgg ccggctcgct ccaactgcgc ctctacgccg
     1561 tcatcggcaa tcattccggc gccgatctgg agtgctacat tccttggctc gaagagcgct
     1621 ttgccgtctc gcaggatatg agccgccaag tgaatgccct gctgcaatac accgccgaaa
     1681 ccgccctgcg ccggctcagc cccgaagaaa tatttaaact gaaaagctac gacaaacccg
     1741 gcctcgaccg catcaccatg cgcgtcaata cgcaacgctc tcgcggctgg gggggctttg
     1801 gctaccggcg ctcgtttccc acgctggaac aactgaccga acaaatgccc atgcgccgcc
     1861 ccaaagctgc ccgcagcgcc gtgatgcccg agtcggcctg ggaaatggaa gatgtggtga
     1921 acgatctggc cgaacgcatc gccgtcagcc gggccaacgt catcattacg ggcagccccg
     1981 gatcaggcaa aagcaccgtt ttgctctcgg ctgtgcgcca cattactcgc aacatgaaaa
     2041 acagcggcct gcaactcagc ttctggcgca tgttggcgca gcgcatcacc gcaggctcca
     2101 aatacctcgg cgaatggcag gaaacctgcg aaacgctcat cgaggaattg caggcctgtt
     2161 ccggcgtact ttgggtaccc gatgcagctc agctcctgct caccggcggc agcggcccag
     2221 aagacagcgt ggcggctttc atgcagcctt ttttgcnnnn nnnnnnnnnn nnnnnnnnnn
     2281 ngcaactcgt gggagaggct accccgcagg aagtggagag catgaagcgc aaattgcccg
     2341 gcttcaccga gtgctttcag gtcattcgcc tgccggagct ttcagaaaaa caggtacaca
     2401 gtattttgga gcggtttgcg gccatggcgc tccagcaaca aaacatcggc atacaacccg
     2461 acgcgctcaa gttggcctgg cgcattctgc accgttacta tccctacgag cagtttcccg
     2521 gcaagggcat ccggttcctc tcgcgctgcg tcagcgatgc ggtcattgcc gaatcagaga
     2581 ccatcacggc ctccgatgtc gtccgacagt ttgtacagca gaccggtttc ccggaaatgt
     2641 tcctccgcga tgatatgctc atggaaaccg aagaactggc gcggtttttc caacaacgca
     2701 tcaaagggca ggaggccgcc atccaacgcc tgtgccgcat catcaaggtg ttcaaaacgg
     2761 gcctcaacaa cccgcacaaa cccattgcca acctgctctt tgccggcccc accggcgtgg
     2821 gcaaaaccgc cgctgccaaa gcgctggccg attatttctt cggcaagggc cgcagccaga
     2881 cacccttggt gcgcttagac atgagcgagt tccagtcggc ctggcaaatc gggcggctca
     2941 tcggcgacgg gcgcgaaccc ggccagttgg tgcgcgaaat ccgcgaacgc cccttctccg
     3001 tactcctgct cgacgaagtg gaaaaagccg atcccgccgt gtttgacgcg ctcctcaacg
     3061 cattagacga gggcatcctc accgatgctg ccggccgggt gacgaacttt cgcaacacca
     3121 tcatcattat gaccaccaac ctcggcgcca acagcgggcc gcccatcggg ttcgccggcg
     3181 agcgctctgc cgccgacagt gcccgcgctg ccattcttag gcatttcagg ccggagtttg
     3241 tcaatcgcat agacgacatc gtggtgttcg agcagctttc caaagcgcat attcaggcca
     3301 ttgccgaaaa ggaattggag gaatttgcac agcgcgaggg cttcatttcc aagtctttac
     3361 accttacttt ctctgaacgc gtggccctgc acctcaccga aaccggcttc gaccccgtgt
     3421 atggcgcccg cccgctgcaa cgggcattgg aacgcgaagt ggcggccccc ctggcggcct
     3481 ggctcctgaa acggcccaaa ctgcgcgagg ccacactgcg cattgatttt gacggcgggt
     3541 tgaaggtgga tgtggaccgg cagtaggcgg taggtgtatg cagtgactgc ccgctgcaca
     3601 ccgcccactg gatgccacat actaaattcg ctcccccaca caacccgaac ccacaaaaaa
     3661 gcatttatta agctcattat caacctgaca attatgaagc acaaattcat catcggcgcc
     3721 gtagtgctgg ctgccggcct gagcatcgcg gcaagtctct ctccggtaag cgacaaattc
     3781 ttcgaaatct cccgcaacat cgagatttac gccaatgtct atcgcgaact caacatgaac
     3841 tatgtggacg aggtggaccc cggcaaactc atgcgcaccg gcattgacgc catggtaggc
     3901 tcccttgatc ccttcaccaa ctacatctcc gaagcagaca ttgagtctta ccgtctcacc
     3961 acggaaggcc gttacaacgg catcggcgct tccagcgaaa aaataggcga ttacaccacc
     4021 atcaccgaac tctaccaggg ctttgccgcc gacaaggccg gcctcaaacc cggcgaccag
     4081 atcatcgccg tggacggcaa agacgccaaa ggcaaagacc ccgaagccct caacgagatc
     4141 atgcgcggct ttccgggcac ctccgtcagc ctcaccattc gcagaccggg ccgcaacgat
     4201 gatttcaaag tctccctggt ccgcgccgaa gtggatgtac ccaatgtgcc gtacagcggc
     4261 atggtgggcg accacatcgg ctacgtcaac ctcaccacct tcacgcagga tgcaggccgc
     4321 aatgtaggca atgccgtgcg cgaactcaaa aacagcgatc ccaacctcaa gggcgtcatc
     4381 ctcgacctgc gcggcaacgg cggcggtttg ctcaacgaag ccgtcaatgt gtgcaatacc
     4441 ttcatcggca aagacttgct ggtggccacc aaccgcggca aaatcaaaga acaggatcgc
     4501 ggcttcagca cctttatgcc cgccgtggac gaagacattc cgctcgtcgt gttgattgat
     4561 aaaaaatcgg cctcagcctc cgaaatcgtc agcggcacca tgcaggatta cgaccgcggc
     4621 gtgctgctcg gccagcgttc ctacggcaaa ggcttggtgc aaaacatcat ggaagtaggc
     4681 tacaatgccc gcgtaaaagt gacgactgcc aagtactaca tccccagcgg acgctgcatc
     4741 caaagtgtgg aatacaaaaa tggcgaaccg gtggacatcc ccgacagccg ccgcgccaaa
     4801 ttcaccaccc gcgcaggccg caccgtactc gacggtggtg gcgtctctcc cgacgtcgca
     4861 ctcaaacaac ccgccgacga acccatcgtc aaggcgcttg tgaatcagca tcttatcttc
     4921 gattttgtca cgcagtactg ccttcagcat ccttctgttg actcggtgga ccgttttaaa
     4981 ttcaccgact tcgacgcctt tttgaagttc gtcgaatccc gcgatttcaa atacgaaacc
     5041 gacagcgaaa agctcctccg cgagttcaaa gacaaggccg ccaaagatgg ttacaaattg
     5101 gacagcgaaa ttgcagcgct ggagcaaaac atccgcaacg ccaaaaaaac agccatcgcc
     5161 ggctaccaaa ccaccatcct cgacctcatc gagcgcgaaa ttgcaggccg ctattattac
     5221 aaaacgggca aaatcaaaat gacgctccgc aacgactccg aagttacgga agccaaaaaa
     5281 gtactcttgg atacggccag atacaaaagc attttgaaaa aacagtagtc aggcgcgcgc
     5341 ttgaagatga tggcttgggg cttgcaactt acggctgtag ccctgagcca atccgtttat
     5401 gtctgaccgc tttccaaaac agttccttac ctttgcgccc caattttcaa tatcatggcc
     5461 gaacagaact ttgttgacta cgtaaaaata tgctgccgct ccggcgcagg aggcgcaggc
     5521 tccgtacatt tcctgcgaga caaacacacc gtacgcggcg gccccgacgg cggcgacggc
     5581 gggcgaggcg gccatatcat cctgcgcggc aaccgcaacg tctggaccct cctctcgctc
     5641 aaataccgca agcatgtcat cgccggcaaa ggcgtggacg gcagcggcaa caactcctcc
     5701 ggcgcctacg gcgaagacat ctggctcgac gtacccctcg gcaccgtagc caaagacgcc
     5761 gaaaccggtg agatagagtt cgaaatcaca cagcacggcg aggagcgcgt actcctgtcc
     5821 ggcggtcgcg gtggatgggg caattcccac ttcaaaaccc ctaccaacca ggcgccccgc
     5881 tacgcacagc ccggcgagcc cggccgcgaa gagtggaaaa tcctggaact caaaacattg
     5941 gccgatgtag gtttggtggg cttccccaat gccggcaaat ccacgctgct ctcggtcgta
     6001 tcagccgcca aacccgaaat cgccgcctat cctttcacca ccatcgtgcc caacctcggc
     6061 atcgtgcagt accgcgattc gcgctccttc gtcatggccg acatacccgg catcatcgaa
     6121 aacgcgcacc tcggcaaggg cctcggccac cgcttcttgc gccacatcga gcgcaactcc
     6181 gccctgctct tcatgatccc ctgcgacacc gaagacatct ccaaagagta cgacattttg
     6241 gtcaacgaac tggaaatgta caaccccgaa ctcttagaca aaccccgcct gctcgccatc
     6301 accaaatgcg acctcattga cgcagaaatg caacaactcc tgctgcccga actgcccgat
     6361 gtgccgtacc tcttcatttc tgccgtagct cagcagggca tcaacgaact caaagacaaa
     6421 ctttgggaaa tggtgtaccg cccggaagac gacgaggagg agatgtaggg caaacaagag
     6481 gggcatatat gtatttacaa atttggaaaa atggagaaaa gggcgtaaac ttgtacgaaa
     6541 ttttaattca acaatatgga acaactcgtg cttcgtcagt tgcagcaatt accacctaag
     6601 ctccagcagg aagtgttgga ctttattgga tacttattgt caaaacaacc gatggcttcg
     6661 gagcataccg cccctgctct tcgatcaaaa gaattggaag aagccattcg tatcgtcaga
     6721 atgggctgcg atatgcgtag ttttggcgat gcttccaatt atcaaacagc atccagaacc
     6781 gaacggtctt taccatttcg cgactaattg cccatgtacc tgattgatac aaacattctg
     6841 atatattccg gggagtcccg ttatgcctcc ttactactcc cctatgtgac agatacagct
     6901 aacggcgttt cactgataag ccatatcgag actttaggat ttcatcgaat tactccggct
     6961 caaatcactt atttcggtag cctctttaaa atactccgag ttcttcctat taatgatgcc
     7021 attgcaaaag aggctatcca aattcgtcaa aacagaaaag tggcgcttgg cgacgccatt
     7081 attgcagcca ctgctttagt ttatgatgta gagatcgtaa cacgaaatac ggttgacttt
     7141 acagcaatac ctcatgtaaa agtgattaac ccaattcctt aaagtgttcc ctcggccccc
     7201 tcacgcaacg cagaccgcag actacaacca cccaagccca tttttttgcg caagtacttg
     7261 taaaatagaa agatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtttta
     7381 caaacaattg gacgccaacg attgcggcgc ctcgtgcctg cgcatgatcg cgcggtatca
     7441 cgggcgttac tactccttgg acaccctgcg ggaaatgacc tacattgaca aacagggcgt
     7501 atccctgctt ggtatcagcg acgccgccga acgcattggt ttccaaacgc ttgccgccaa
     7561 actcacctac tcgcgcctgt gcaaagacat cccgctaccc tgcgtggccc actggaatca
     7621 ggagcatttt gtggtggtgc ataaagtaac cgaacgccat gcctggatag ccgatcccgc
     7681 cgccggaaag tttaaaatct cccgcgagca attcctcaaa agctgggccg gcgacctcga
     7741 aaacggcgaa ccctccggca tcctgctgct gctgcaaccc acccccgaat tttaccaacg
     7801 cggagaaacc aaagccgaca aaggcgggct gggctatgtg tggtcgtatt tcaggcagta
     7861 cagttcctac ctgctccaga tagcggccgg cctgctcatc ggcgtgctgc tgcaaattgc
     7921 cttccccttc attttgcgcg aagtggtgga ctccggcgtg cagtcggaag acgtccaact
     7981 catctacgcc gccgtgctgg ggctgttcgt actctttgtc tctcaactct ttgtggaata
     8041 tgtgcgcggc tggattttgc tgcatgtggg cgcgcgggta aacatcaacc tcatctccga
     8101 ttttttaatc aaactcactc gcctgcctct ccgctatttc gacaccaaag tgaccggcga
     8161 cctcctgcaa cgcatcaccg accacgagcg ggtgcagcgc tttttgtctt ccacctcgct
     8221 gttttcggtg ttttcattct tcactttcat ctcgtttttc atggtactgg ccgtttggaa
     8281 taccggtgtc tttttgctct tcgccggcgg taccttgctc agcgtgcttt ggttttacct
     8341 cttttccacc tatcgccgcg atctggacta cgcacgcttc gatcaggcca ccgacaacca
     8401 gaacaaactc atggagttga tcaacggcat gcccgacatc aagctccaca acgccgaaaa
     8461 gccccgtcgc tgggcttggg aacgcgcgca ggccaagctc ttccataccg ctttggacac
     8521 cttgcgagtg gatcagatac aaaaaaccgg cgcccagttt atcaacgaag ccaaaaacat
     8581 catcatcctg ttttgggtgg cgctgcaagt ggtagaaggc cacatgacac tgggcatgat
     8641 gctgggcatt cagtacatca tcggccaact caacggcccg ctcaatcagt tcgttgactt
     8701 catccgctcc tatcaggagg cgcgcatcag tctggagcgc atgaacgaaa ttcataccaa
     8761 agaagacgaa gaacgcctcg aagacaagat cagcctgctg cccgactggg gcgacctcat
     8821 cctcgaccgc gtctcttttc aatacgccgg gccgcactcg cccctgattc tcaaaaatgt
     8881 acgcctccgt attcccaaag gcaaaaccac cgccatcgtg ggcgccagcg gcagcggcaa
     8941 aaccaccctg ctcaaactac tgctcaacta ctaccaaccc aacgaaggcg ccgtccgcat
     9001 cggcgacatc aacttggcca atatccaaaa ccggctctgg cgccagaaat gcggcgctgt
     9061 gcttcaggat ggctatattt tcaacgacac cattgcccgc aacatcgcgc tgggcgacga
     9121 ggtgattgac aaataccgcc tccttcaggc tgtgaaagcc gccaacatgc aaacctatgt
     9181 ggaatcgttg ccgctgggct acaacacccg catcggcgcc gacgggcagg gcctgagtct
     9241 gggccaaaaa cagcgcatcc tcattgcccg cgccgtgtat aaatcgcccg attacctcat
     9301 tttcgacgaa gccacctcgg ccttggatgc ctacaacgaa atgatcgtga tggaaaactt
     9361 agccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc atggtcatcg tggcgcaccg
     9421 cctcagcacc gtcatgcacg ccgattacat cgtggtactc gattcgggcg aggtggtgga
     9481 gcagggtacc cacgcacagt tggcctacca gcgcggcgcc tattatcatt tgattaagaa
     9541 tcagttggaa ttgggagcct gagatcggat cgttcactaa gcatcccgac gaccactaaa
     9601 aactcgcaca tcacacaatc tgccgtctta tgaaattccc cattcctgcc ctcagcgacc
     9661 gcaagctcct gtttctcttt gccgtagtac tcgcctttct ggccttcccc atgcgcgagt
     9721 ggtaccaaaa cggcgttatt tgggccgatg cagagggcta ctacatgtac ctgcctggcg
     9781 tttttattta caacggcttt gaaaaaatac ccgtacgcgc tgatggccag tttccgcatt
     9841 atcccggcac gcaaaagcgc tttaccaaat acacctgtgg cgtagccatg atgcaaacgc
     9901 ccttttttct ggctgcgcac gcccgcgccc gcgccgaaga caaagccgac ggatacagca
     9961 cttactacat atatggcgta ctctgggcag ccctgtttta cgcggtggcc ggcgtctgga
    10021 ttttgatcaa aaccctgcgg cgacacttct cacagacagt agcctggaca gcggtgatgg
    10081 ccgttttttg gggcagcaac ctctaccatt acaccgtaca cgagccgggc atgtcgcatg
    10141 tgtattcgtt ctttttagtg agttgtttgc tttggcttac gccgaaattg tacaaagaaa
    10201 ccattcagcg caaatggatt tttctcattg gcctggttgc cggcctgatg gtactcattc
    10261 gtcctaccaa cgtggtgctg ctcttatacc cgggcttatt tggcgtcggt tcctggtcgg
    10321 atttcaaagc ccggcttcag tttttcgggc ggcacatcgg gcaattatgg ccggccgcgc
    10381 tgctgattct ggccgcctgg ataccgcaaa tgctgtattg gcgctacatg agcggcaact
    10441 ggatcatgta ttcctacggc gatgagggct tcatttactg gaaccatgcg cgcatggcgc
    10501 aggtgttgtt cgacatcaaa aacggctggc tgctttttag tcccatgatg gccattgcat
    10561 tgatcggcat ggtagctggg ctgtggcgca accgctacaa cgagcgttgg atcggcttgg
    10621 cctggctcat cagttggtac ctgtttgcca gttggtgggc ctggtggttc ggcggcgcgt
    10681 tcgggcatcg cagttttata gacttgtacc cgttgctggc cataccgttt gcggcagtcg
    10741 tgcaggcggt atggagtact cgcagcatcg tgctcaaggg gctgtttttg ctgctcttgg
    10801 gagcgctcgc ctactacggt ctcggcctca cgagccactt tatcagcccg cactacgagt
    10861 ggcctacctg gcaggcggcc atagaactga tgctgaaggg gaagttttga tgtttggaaa
    10921 ttggcatgaa ttcttaattt tgatgaaaaa ttcatgagat cagaagccga ccacatagaa
    10981 ctccgcagcg aagaagtgca ggaaatcctc ggcacccccc cgggttggct ggcacgttgg
    11041 ggcaccttgg ccgcgctcat cggcgtagta gctttaggat ggctcggctt ttggttggaa
    11101 atgccacaaa ctttggaagc cgacgtcaaa attacctcca aagacccccc gcgcaggttt
    11161 tacgcacctt ccaccaccgt gctcaacgac atcattttcg gcaacgaatc caaagtggac
    11221 agcggcgcta ccatcctgca tttcaactcc aaagccaggc ttgcgcatgt gctgtctctc
    11281 gaaacagagc tgcttcaggt aggaaacgat caacaaatcc tgtccctgaa catccaacga
    11341 gacctcatcc tcggcgaact tcaggaagac tactacgact ttttggaaaa atgggaccag
    11401 tatcatttcg tctccaatcg ctccaccgac aacagcggtc gcagcgaagt cgcctcccgc
    11461 atccgggatt tggagtctga aatannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn agcccaggcc cgctacgata aagagttgac cttgctcaac agaggcgtac
    11581 tcacccgcga agaactcaac ccaacccgca tagaacgaga cagttacgag cgacaagtac
    11641 aagatgtaca gtcttccatc cgcagcaaaa gcaaccagat ttccatgctc cgcgaccgct
    11701 cgcgcagcaa cagcggcagg gccaaagcat tagaagacca aagcgcggca ttcggcctgc
    11761 tcaaagacag tttcgcccgc ctcagaaacc gggtttcgga atggaaacgc gaatacaccg
    11821 tcgaatcgcc catcagcggc acggtattgt ttgccaaaga caaccttgca aaagggcagt
    11881 ttgtgcagcg cgattccctg ctgctcgtca ttgtgcccgg caaagcatcg ggcacggtag
    11941 caaagatgga aattgtgccc gaagaggctg gccagatcgt cagaggccag aaagtgcaga
    12001 tcaaattgta cgattatccc ttccccgact atggcgccgt gtgggggcag gtggagggca
    12061 agggcaaact gccttccggc ggaaaaatcc ccgtagaggt gcgctttccc ggcgacagct
    12121 tagtgactac caaagccggc ctcattcagc ccgaaggcga aatgcggggc aaagccacca
    12181 tcatcctctc acgccggcgg cttattcagc gcatcttcgg catcggggaa ggatagcacc
    12241 cgaagatatt ctgccggtac ttcatcggta agccacacgc cgttttcgga tataaaaaac
    12301 tggaaacccg cgttttgcat cgctccgccc cgcacttcca atatcaccgg acgaccatgc
    12361 cgactgccca cgctgcgcgc tgtttctaca tcgcgactca ggtgtacgtg gtggcgttgc
    12421 cgcttttgca aacccgattc aagtatagat ttcaggaagc gctccgccgt gccgtgatac
    12481 aggatttccg gcggctcctg tgccgaatac ccgagtgcta cctctaccga atgcccctgc
    12541 gcagcccgga ttctgccctt cgtttcgtcg agtacaaagc gctttttgtt gttgttttcc
    12601 accacgaatc gaagggtctc tatatcgagc ggcgttcctg cggcattggc tttttggagc
    12661 aatgtgggca cgtcggtcca gccttgttcg tccaatatga tgcctatcaa ttggggtcgg
    12721 tgccgcagca cgagactgag aaatttgctg agccggatga gttctttagc ttgcatgact
    12781 ttttttgttt aaatcgctca ggatacggcg ccccgaaagg aaaagtttcc gggttcaagg
    12841 tttcgggttg gaacggctgc cggctgccgg ccgcgtttcg ctcgcgttta ccgcgccacg
    12901 gcgcggctac cggtccctcg cgttcgcgga atttcgcgcc cttcgctaag attcgcgtta
    12961 aaaaactcgc gttcgacgat aaactgccaa accgcgccac aggcgcggcc ctctccgttg
    13021 gcaagataaa ctgcgcagca gattactacg aagtgagata aactgccgaa ggcagatcac
    13081 ccacgataaa ctgcgcagca gatgacgaaa cattttacct ttgccgccga ttcagcgcgg
    13141 ccctgcgccg ccacatattt ttacatcgtt taaaccaata aaaccatgcg atttggtacc
    13201 aaagccatac atgccggcgt agagccggac cccgccaccg gcgccgtaat gacgcccatt
    13261 taccaaacct ccacctacgc gcaggaggcc cccggcgtac acaaaggcta cgaatacgcc
    13321 cgcacccaaa accccacccg ccacgccctc gaacaaaacc tcgccgccat cgaaaacggc
    13381 acggaagcct tctgtttcgg cagcggcatg tcggccatgg acgccgtgat gcgcatgctc
    13441 aaccccggcg acgaagtcat tgccaccaac gacctctacg gcggctctta ccgcctgctc
    13501 accaaagtgt atgagcgctt cggcgtgcgt tcgcacttca ccgacatgac cgacccggcc
    13561 aacgtggaag ccctcatcaa cagcaacacc cgcctgctct ggatcgagac gcccaccaac
    13621 cccatgctca acatcattga cattgaggcc gtttgcaaaa tcgcccggca gcacggcatc
    13681 ctcacctgcg tggacaacac cttcgcctcg cccttcctcc aaatgccgct cgacctcggc
    13741 gccgacatcg tggtacactc tgccaccaag tacctcggcg gccactccga tgtcgtcctc
    13801 ggcgccgtca ttgtaaagga tgaggccctg gcccaacaac ttcgcttcct ccaaaacgcc
    13861 gtcggcgcag tgcccggccc gcacgattgt ttcctcgtgc tgcgcggcat caaaaccctg
    13921 cacctccgcg tagagcgcgc ctgcaaaaac gccaaaaaag tggccaaatt cctcgccgcc
    13981 caccccaagg tgcagcacgt ttattacccc ggcctcaaag accacccggg ctacatgatc
    14041 gcctcgcgcc aaatgcggtt ctacggcgct atggtctcgt tcatcctcca aaaagacacc
    14101 gtggacgacg ccatgaaagt actcagcggc acacagattt tcaccctcgc cgaatcgctc
    14161 ggcggcgtcg aatcgctcat cgggcacccc gcctccatga cgcacgcctc catcccccgc
    14221 gaagagcgcc tcaaaaccgg cctcagcgat tcgctcatcc gcctcagcat cggcgtagaa
    14281 gattttgaag acctcatcga agacctcgaa caggcactga gattggtata gttttttttg
    14341 gcgcccgccg ccacctgcaa cgaagggccg acagacaaca gcctcaaaac ggctccacct
    14401 gtcggccctt cgcactcatg gcggcgccgg gtcatccgct cacatggccg cctcctttta
    14461 ttacccgccg gcgtccattc ccgctgctaa agctaacagc tctattcccg accgccgcca
    14521 ttcccgctgc tgcccctggc gcaatcccac cgattcaagg tttccggttg gaacggctgc
    14581 tagccgcaag ccacgctgag cgtacaaatc gcggtttccc tcatgttcgc ggaattttgc
    14641 gatcttcgct aaaattcgcg ttaaaaaaaa cgcgttccgg gcctcgccac gacgaaggcg
    14701 tggttcaagg tttcgggttg ctcactacgc acccctcact actcacccct cactactcac
    14761 ccctcacccc tgaaaaccgc ccaccgccta ctggtttccc tcgcacttac cgcgccacag
    14821 cgcggccact gcatactgca caccgcttac cgaatctcct ccttcaacaa ccggtcaata
    14881 tccttcataa actgatctac cgacttgggg tccgtgccgt cgcaaaatgc gcgcacatgg
    14941 cgctttgtgt ccaccagaat gagccgcccg ctgtgatcga agccgcccgg cgctgtagca
    15001 tcctcaatgg ccacgctgaa atagtcgtcg gcaatgtcat aaatcgccgc ccggtcgccg
    15061 gtcaccaaat gccatcgcgg cgccgacacc cccagtcccg cagcgtattc gcgcaagcgg
    15121 cccaccgtat cgcggcgcac gtcaatgctg tgcgagagca ataccaaacg gtcttcattt
    15181 tcgtagcgct catacaggcg caacatctgg cgtttcacct tggggcaaat cgtggggcag
    15241 gaagtgaaga aaaaatcggc cacatatatt ttgtctgcaa aagtggcatt cgtcacctcc
    15301 tggctgtcct ggttcacaaa agagaaatcc ggcaccttat gatatagggt gtcgccgtcc
    15361 acaatgtcta tttcgcccaa aatgggcagc ttgccctccg ggtgttggca agaggctgcc
    15421 gccaggccca gcaccggcag cagcaacaga aaaaatctct tcatcattga gcgtttgttt
    15481 cagatgcgtt ttgcgggagt tgtttcttca gcgcctcagc cgcctcgatg ctgccgagca
    15541 tagcgttttt cacttccgtt attttgccga tttcatcctg aaggtactgc atgatgtctt
    15601 cgtgcttttt gccgttgcgc aaatcgggca attgctgaag gccgtacatc cagtcccaca
    15661 tgactttgtc ggcggcttgc aggtcgtaca gcgcttttct ggttgcatcg cgcagttcgt
    15721 ccgtcaaagc agtatcggcc gtagccgcct ccatttgttt agacaaggca ttgatgtcgc
    15781 tcattttcgg catcacctca tcatgcacca ccatcatttc gtcccagagc ttttgctgct
    15841 ctgcttcctg tgcctgattg gtgccgcatg cgctcagccc ggtcagaaag gccaatgcga
    15901 gcaggatcat tttaaagttc atttttcagt attttaggct gcgaaggtac ggcttgaaaa
    15961 tgtatcaaca tactgcaaaa acttctttcc tttgcgnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgaa
    16081 atcgggaatc cgaaatctta aaatgcacat accatgaccc gactcagcgt caacatcaac
    16141 aaaattgcgc tcatccgcaa ctcccgcgga ggcaacatgc ccgacctcgt gcaaatagcc
    16201 aaagactgcg aagccttcgg cgcacagggc atcaccgtgc atccccgccc cgacgagcgg
    16261 cacatccgtt tttccgacat cccgctgctc aaagccgtga cgaccaccga gttcaacatc
    16321 gagggcaatc ccaccgaggc atttttgaag gccgttttgg aacaccgccc gcaccaatgc
    16381 acgctggtac ccgacgcgcc ctcggcactc acctccgatg ccggctggga caccgtgcgg
    16441 cacgcatcct ttttgaaaga ggtttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
    16501 gcagttctat ttttatcaat gccgatgaaa aaatggtgga aggcgccaaa gccctcggcg
    16561 ccgacagggt agaattatac accggccctt ttgccgctca gtttccacaa gatcccgaag
    16621 ctgccgtagc cccgcatcgt cgttgcgccc gcatcgccgc cgaaatcggc atcggcctca
    16681 atgccggcca cgacctcaat ttagacaatc tgcgcttcta ccaacaacac accgaagccc
    16741 tgctgnnnnn nnnnnagtct ccatcggcca cgccttagta tgtgacgcgc tttactacgg
    16801 cctctctaat accatacaaa tgtatctcag gcagctttcc gcggcatgaa acgttcttaa
    16861 cttttattta aggaagcgaa aacattttct taaaccctct tttcccaaaa gccactgaaa
    16921 accaaagtat aacgacgcgt ttcagccgca ataaattggc tttgcgctaa aaaaaacgcc
    16981 gcgaattgcg cggtctttta caaaaggcat atttttggca tcatttttca tttttttaat
    17041 caaaattcgg attagtatga aaaaactctc actggttctc gcgctggtac tgttcgcctt
    17101 tggggcagca gttgcgcagc gaaccgtcag cggcatggtt tccgacacaa aaggagaacc
    17161 gctcattgga gcgagcatcc ttgtgaaagg aaccgtcaca ggtacggtaa ccgactttga
    17221 tggaaagtac tccatcgctg tacctgccgg cagcaccact tttgtggtca gctacaccgg
    17281 cttttctacc aaggaagtgg aaatcggcgc ttccagttct atcaacatta ctttggaaga
    17341 cgctgccgag cagctctccg aggtggtggt gacaggtctc ggtattcgca aagaaaagaa
    17401 agctttggga tacgccgtga ccactttagg ctctcgcgac attgagcttc gccctgaagc
    17461 cgacgtttcg cgggtgcttc ggggaaaagt acctggcgtg gacatttctc agacctccgg
    17521 tctggccggc tcaggtacca atgtcatcat tcgcgggtac tcttccatta cgggtaccaa
    17581 ccagccgctt ttcgtggtgg acggtattcc gttccactcc tccaccaacg cagaccgcgg
    17641 ttttgattcc ggctcggctt ccgcttccag ccgcttcctc gatcttgatc ccaacagcat
    17701 tgctgaagtg agcgtactga aggggttgag cgccaccgta ttgtatggcg aggctggccg
    17761 caacggcgtt attcttatca cgaccaaaaa cggcgttccg gccaattcca acaaaaaact
    17821 ggaaatcacc gtgaaccagt ctttgtacgt caaccaaatt gcttccttgc cggaagatca
    17881 ggacttgtac ggcaacggtt tcgacaactt tgcctctgcg gctttctcca actggggcgc
    17941 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    18001 ctatgaccgc gctgcgctga acgctgtgtt cccgcagtat attggtgcgc gttatgctta
    18061 caaagcgtac gacaaccttc agaacttctt caccaagggc ttgatcaaca acacggctat
    18121 caatattgcc tctcagttgg ccaacaacac caccttgaac gtcagctatg ctttccgcga
    18181 cgataccggt tttattccgc aagagtacaa ccgctacacc aagcataact tgggtctggg
    18241 tttgcaaaca aaactcagca atggcttcaa gatcagcagc acgttcaact atgtgacttc
    18301 tgatcgtatc tcgccgcctt ccgacatcag cttcagctcc ggctccgacg gcagctttac
    18361 ttcgcttttc ggccacattt tctacacccc gcgcagtgtg ggcttgtatg aattggagta
    18421 tgagaacccg gtggacaagt ccagtatttt ctatcgcggc ggcaacgata tccagcactc
    18481 gctctggacg ctcaacaact ccggcgattt ggaagacctg agccgtttct tcggtaatat
    18541 gcaggtttct tatgaactga ccgattggtt gacggctgct tatcgcgtag gcgttgacgg
    18601 ttattctcag cagcagcgct actatctgaa caaaggcggt attgacgttc cgaccggtgt
    18661 tttgagaact tcaaaccgca gcaatacgat tctcgaccac ttgttcagcc ttgcttatga
    18721 cttcagattg ggcgattcat gggtattgga cggcgttgct gggttcaaca tgcgccgcga
    18781 tgtgctgaac agaaccttcc tcaacagctc tcagcagttt gtgtatggcc ttttcacgca
    18841 cgataacttc attgacaaca caagctcctc cgataagttt gaggaaaacc tcatgggcgc
    18901 cttcctgacg gcttctttgg ggtacaagag cttcctgtat ttcaacttcc aggcacgcaa
    18961 tgactggact tctacgcttg aaaaagagaa ccgcaacgtt ttctatcctt ccggttccgt
    19021 ttctttcatt cctacagaag cttttgccgg ccttcaaaac agcaacctcc tcaactatat
    19081 gaagttgcgt tttggctatg gtacttctgc cggctatccc gatccgtatc agacgcgtaa
    19141 catcctttct accggtacgc gcatttttgt aaacgatgga ggcacggtga tcaacaccaa
    19201 cagcgtttcc aacgtattcg gcaatccgaa tctccgtccc gaattgcaca aggaattgga
    19261 gttcggcctc gaagcgcgca tgttcaaaaa ccggctcggc attgaccttt ctttgtacaa
    19321 caagacctct tccgacctca tcattgatca gcctctggac ccgtccaccg gcggtacatc
    19381 cacaacggta aacgctgcgt ccatccagaa taaaggcatt gaattgggtg tggacattac
    19441 tccaatccag agcaagaact tcacctggac tttgaacgcc aactttacca agaatcaagg
    19501 catcgtgacg gaattgctgg acggggtgga tcagatcgcc ttctcaggct attccaacct
    19561 cggcaacttt gccattcctg gccgccctta cggcattatc ttgggagata aaattttacg
    19621 cgatgacaac ggcaacccga tcgtaacaag tgcaggtgtg tatcaatctt ccggtgagat
    19681 cggagaactc ggcgacccaa acccgaatta tctgctcaac ggcggtactt ccatcaactt
    19741 taaaggtttt tcactgagcg ctttgttggc gtatgtggac ggcggcgtga tttacgctac
    19801 cacgccttct accttgatgg gccgcggcgt gcttcaggaa accggcttcg atcgttttgt
    19861 tcccttaatt gctccgggcg ttaaggcaga cggtacgccc aatgacatta tgatcaacgc
    19921 ttcttctcat tactgggaaa atggaggcgt tttccacgat gaaatgcgta cctatgacgc
    19981 ctcctatgtg aaactccgcg aggtatcatt ggggtattca ttcccgaaga agattttgga
    20041 aaaaactcca ttcggcagca tgacgctggt attctctggc cagaacctgt ggttccgggc
    20101 attgggcgtt cccaagggcg ccaatttcga cccggaagta tccagccttg gcgtaggcaa
    20161 cggtcgcggt ttcgagttga tgaacgttcc gacgtccaag caattcggcg gcagcctcaa
    20221 gttcacattc taattattcc aaaagcaaac tttgaatcat gaaaagattg tttaaaatat
    20281 caattatcgc aagcttcctc atctttttgg gggcttgtga aagcacggaa ttggaggggc
    20341 tgctcaacaa ccccaacgcc gtgactcctg aaaacgcaga gttagatctg gtattcaacc
    20401 gggtcatgct cgatttcaag agttttgttt acgaagcgca tgcgcagacc gctccatata
    20461 ccagaatgct ggccatgacg ggaggttatc agtacaacag ccaagatcag ccgcagtctt
    20521 ttgactatat atgggaactg gcttatgcaa atttattgcc ggacatcgaa ttggtcattt
    20581 catcggctac ggaaaaaggc agtacggtgc atgccggcat cgccaaaatc ctgaaagctt
    20641 atgtttatat gactttggta gacttgttcg gtgatattcc ttattcagaa gctctgggcg
    20701 gcgcgagcaa tcccagcccc aaggctgatg acgacaaggc cgtttatgaa gctgccgcgg
    20761 ccgtattggc acaaggtatt gacaacttga aaacgcctgt tggctcgccg gtaaacgacc
    20821 tgtactataa caaaaacgtg gctcgttgga tcaagttggg caaaaccctg caactgcgtt
    20881 accacgtcat gacccgcttg tcgggcggtt ctgcaaccgt tgttaatgca ttgctggccg
    20941 aaggcgacct gattaattca gcggacgagg atttccaatt caaatacagc aacaaccgct
    21001 ccaacccgga ttctcgtcat ccggcctata gcggagctta tgaatcggct gccggtgctt
    21061 atctctctan nnnnnnnnnn nnnnntgttc tttgccgata aaacaacgga agacccgcgt
    21121 ctgcgctact atttctaccg ccaggattgc gatgaaagag aagaagacct cttcaccctg
    21181 ggttgccgca cgcagcctta tcctttccat tgggcggacg gttacccgtt ctgtacggca
    21241 ggtctgatgt tctcgggcgc caacaatcaa tatctgggct actggggccg cgatcatggc
    21301 gatgcatccg gtattccgcc agacaaccag aagcgtacag ctattggcgt ttatcctgcg
    21361 ggaggcaagt ttgatgccaa caactgcgcg catgtgcaac gcttgggtgt tgacggcctc
    21421 aaaggcgcag gcatccagcc cattatgatg tcttcctggg tggacttcct gaaagcagag
    21481 gctgcgctga ccatgtcggg cgtagccggc gatccgcgta gtttgcttga aagcggcgtt
    21541 cgcaagtcta tctccaaggt aatggcattt ggcgcagccg atgcaaccag cgcttatgtg
    21601 ccttctactg ctcaaattga tgcttatgta gctgaagtgt tggccgacta cgacgctgct
    21661 gctactaccg acgccaaact caacgtcatc atcaaggaat accacttggc attgtggggc
    21721 aatggcctgg aagcgtacaa caactatcgc cgcacgggca aacctgccgg tatgcaacct
    21781 actttgcagg cagatgcagg tacgttccca cgcctgctgt ggtatccggc caactatgcc
    21841 aacctgaacg ccaatgccga tcagcgcaac ctggctcaac aggtgttctg ggataccaat
    21901 gctgccggct ttatcaagta attcaatttt ttcggtgaga accattcgct tcggcgcgtg
    21961 ttctcaccgc aaactaaaaa atgcgaatgc aatgaaaaaa atattgattt catttttcag
    22021 cctgtctttg ctgttcctgg ccttttcctg cgaacaggag cgcgctttcc ctgagtttga
    22081 ggacctccaa tacggttctt ttccccggcg attgtcgttt acaggcgaat attttatcac
    22141 aaacattgcc acttctaaaa tcaccttcaa ggttgaattt tatgatgaaa atcagggcaa
    22201 aaacgtagag tcttattctt gggccgtcag ctatattgac cgcaccaaca acggcgccaa
    22261 caaccgcccg gcgcaaacga tccgcaccgt gaatgcttct cagttcgtta ccaaccctga
    22321 cacggggctg ccgtcaacga cgattgagat tcccttgcaa gacgttctca acctgtttgg
    22381 gttctctgct aatgacgtca atggcggaga ctcgttcatt tttaacgcta ccctgcgtcg
    22441 tacagatggt aaagtattta cgtcggtcaa taccaacgac cttctcgaag gtcagccggc
    22501 gttcaacgct ttcttccagt tccgtgccga cctgaaatgc acgcctgcgg ctctggcggg
    22561 tacttacaac tactccactg taggctggtg cggtactact caaacgggta ctacccgctt
    22621 ggtggctacc agtgtttccg gtgttttcaa cgttgtgagc ccggacgctc cgagcgatcc
    22681 ggatgcggcc gacttctcgt ttggcgctta tgctgcctgc tacggcgccg gcagtacgct
    22741 tcccggcggc aatctccaga tcgttgaaaa ctgctacggc cttcgtccta tcggtttgag
    22801 ccgctggggc gaagactact agtacaactc agtgaccgtt gatgggccca acctcatcat
    22861 tgactgggaa aatagctatg cagaaggtgg cgtaaccact ctgacacgta cagacggcac
    22921 caactggccg ccgctgaaaa agggctaaaa tcatctttag tcttgaatat gagtcacttg
    22981 ggattgccct gggtggctca tatttttttc agatcattta ttgggaggta cggggcaggt
    23041 tcttgttttt tgagactacc ttgcgggcga atctccgcct tatttcttta tcatctttga
    23101 attttaagtt tctcatgcgt tcaactctac gtctcctttt attgagcgtt gtgctttttt
    23161 gcacggtatc tgagccggca gcacaaccca cgaccggttt gacttctttc accgcagggg
    23221 agccttctcc gggttataac ctgatttttc cacataatca gccgcatgtc tatttgctca
    23281 ataactgcgg tcagattgtt catcgctgga acagcccatc agtgagaagg ccgggcaata
    23341 cggcttattt attgccggat ggcaacattg tgatcgccag ccggtatttt gcctcggtga
    23401 acgacccgat ctgggcgggc ggcnnnnnnn nnnnnncggc gaatatgtgg aaatcagaaa
    23461 ctgggacaat gatactttgt ggagttttac gctcaacgac acactgcggc gtttgcacca
    23521 cgacattgcc ccgatgccca acgggaacat actgatgatt gcctgggaac tgaaaacctt
    23581 tgaagaggca gtacaagcag gacgtaatcc ggcactcatg ccacagcata aattctggcc
    23641 cgattatata ttcgagatca accctgccac caacaatatc gtgtgggaat ggcatgtttg
    23701 ggatcatctc attcaggatt ttgaccctac taaagataat tttggctcgg tggcagcaca
    23761 tccggaactg gtagatttga actacgacga caacggcggc aatcccgact ggatgcactc
    23821 caatgctatt gactacaacc cggtgctgga tcagatagcc ttgagtgtgc ccacttttaa
    23881 tgaaatttgg atcattgacc acagcactac cacagaagaa gcagcgggcc acaccggagg
    23941 cagaagcgga cggggaggcg atttattata tcgttggggg aacccctctg tttacggagc
    24001 tggagactca acacatcaac gattgtttta tcctcatgat attcactggg ccgacgattt
    24061 ccttgacgct tcgcatccac attacggcaa attggtggct tttaacaatc aggtaggccc
    24121 caatttttcc tctgctgaaa ttttcaaccc ggcgttcgac acgcttactt ggtcataccc
    24181 tatggcagag ggaagatggt tgcccgttca atccgatcag accgtcacgc atcctgtgcc
    24241 cacccgaatg tattccgacg gcacttcaag catacgtttg caacccaatg gcaacatatt
    24301 attgtgctcg ggacgccagg ggtacgcatt cgaactgaat gaggagttgg acattgtatg
    24361 ggaatatatt actcccttgg tcgggggtgt ccccattgcg cagggaagta cggctaccaa
    24421 taatctgact ttcaggatgg agcgctaccc tgaaagttat ccaggcttgg agggtaaaga
    24481 cctgtcgccc aaaggttata tcgaactaaa tcctgatact acgttctgtt ccctgatttc
    24541 cagcatagga gaaaccgata ctgaaatgcc tgattggaaa ctttacccca atccggccgg
    24601 cgacgaatta tggatagaat ggaacggaga tgcgcccgcc tttctggagt tttttgatgc
    24661 tatgggccgc ctccaacata agacgaagcc ggtggggaat acaccgacgc gactatctgt
    24721 aacagattgg ccttcaggag tatattatct ttcagacagc agagggaaca ccgcccggtt
    24781 cgttgtccgg cgatgaaatg agatgaagtt gattcccaaa gcagatatca acgttttatc
    24841 catttttgca taatcacctg gccgtcttcc tgtagaaata gaatgaagta caggccggaa
    24901 gatagatgct ggacggcgat gttccattcg gcagcgcgaa tacggtcgct cataagcacc
    24961 ctcccgtaag tatccaatac cgaccaaaac cgaatatcgt tgccgatgag ttgtacatgc
    25021 aattcatcgg tagcggggtt gggaaacaga tgagcggctt ggggttgatc cgcgggcaag
    25081 tcggaggtat gagagatgat ttcaggatca acgaaaatgt cgccgccggt agtagagcct
    25141 tccaccacaa ctaatttttg accggaaact acattcaaca gccgatcaat atgcccggtc
    25201 ggccaggtaa tgagtataga atctgcctgt gtagcgctgc cgatgccgat gatttccgta
    25261 tcggaatttt gccccaaaaa gccaatccca cagtgcgtgt atcgcatttg gtaatggctg
    25321 ccggtataaa tttctatttt cgcgccgatg ccgtcccggt tgctaagaat gccttgcagt
    25381 ttgattttga gccagttatt ggatgttgtt ccctgatgaa gccacagttg agatgcaaaa
    25441 ggcgccgtat tggccaccat aatgtcgggg tagccgttgt tgtcaaaatc gccggtcgca
    25501 ttgctgaaac tggtcaccgt atcgcccaca aaccccgcat tcggttgcaa gaaagaacca
    25561 ttgcccatgt tttcataaaa agcgctggat atgacatcgg ccccggccag cattccactc
    25621 acatacaggt cgaggtcgcc gtcgttgtcg gcatccaaaa aaacggaacc ccagccgatg
    25681 ctgttgaaag cgactccggc agaagctgct acttcttcaa acaccacctg cccgtcgtcg
    25741 tccgggcctg aatttcgcag caaagcgttg ccttcttccg tattggaaat ataaatatcc
    25801 agccaaccat tgttgtcgta atctcccacc gcaacgcaca tggcgttcat ggcgaggttc
    25861 gtatttgtag ccacactgac attggagaat gtgccgttgc cgttattgcg caacagcgaa
    25921 ttgattctca ggcggtcttg agcagtataa atatcgggcc atttatcatt gttgtaatcc
    25981 agaaaagccg agcaaaaagg cgtcttgcca ctatcggcgg cttgcgcctg aggggtcacc
    26041 tctgtaaaag tgccgttgcc gttgttccga aaaagtctgt tttcattggg caaccccgat
    26101 agctgcaatt ttcgttcatt gtaatacaag tcgagtaaac catcccgatc atagtctccc
    26161 cacacagctc caaaagtatg aaaagcgtcc aagggcagtc ctgccgatag agtaatatct
    26221 tcaaaagtca gattgcccgt gtttcggtac aaacgattga cgccttcgta attgcaaaca
    26281 aacaagtctt tatcggcatc attatcaaaa tctacccaca gcaattgttt tacctcgtcg
    26341 agatgaggta ctaagggttg caaaagcgtg aactgcccac ccatattcaa ataaaagcga
    26401 ataggttgcc cagactcaga acctaatgta atgtcgtcca gtccgtcgtt attgaagtca
    26461 acgcagctca caccgcaacc gggcgttgtt gagttgtaaa tatgctgaat gcctctggct
    26521 gcggctgcat cctgataaag aatttgagca tgaagggaga taacaattcc aaagcaacat
    26581 gcagaagtga taaaaaactg cttcatggca agcatatttt taggctgaat gaaaggatgt
    26641 cattggatgg taatnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgccg gcttttgttc
    26701 tgttgtatct ttctgtggtt cgggcggcgc agacacataa ttgtacacca cctgcggcga
    26761 cggggcggta acaaccggcg ccggttctgc cgcggccaca tttttctgaa tcctgaaatg
    26821 caccttcagc gtgttgtgaa aggtgacttt cagtttgcgg atgggcggca catcaaattt
    26881 aagcaacttt gctaagcgaa cggcttcctc gtcacagcca tgacccacgc tggtcagaac
    26941 ctccacatcg gtcacattgc ctttataatc aatctcatag cgaatggaga cggtgccttc
    27001 aatgcctttt ttgaatgcct ctgccggata gcgcaagttt tggcgcaaaa actcctgcat
    27061 ggctttgggg ccacccggat agatggggcg gcggatgaaa tttttttctt ttctgtcctt
    27121 ttgcatctgg caaaggtagt taaatgtgct gatgatggta gtgtagtgaa ggggtgactt
    27181 tcgcccgaaa atctcttgta gtcacccctt cactaagcca agacggcgcc caatcgtcac
    27241 tttgtactta gctttgtcgt tcaaatattt aaaaagcatc atgaacaacg agatcaccca
    27301 tgcgccgcaa ggcgccgatc aacagccgcc cgcccccggc agcaacatct gggggccgaa
    27361 atttccgctc tttgggctgg ccgtcatcct cctgttcgcc ctactgatcg gcgcccgcgc
    27421 ctgctacctc ggcgtaccgc tgggcgatgt tttcaaaaat accgatcctg ctcccaccac
    27481 cattgacacc acaagcactg gccaatgaag cgctccattc ctttcaaaaa ataccagggc
    27541 gccggcaacg attttgtcat tgttgataat cgggcggaaa ccttcatcgg gcgcttcgac
    27601 acggagttaa tacaacggat gtgcgaccgg cgcttcggca tcggcgccga cgggctgatg
    27661 ttgctgcaaa accatcccgg atacgatttt gaaatggtgt atttcaacag cgacgggcgc
    27721 gaaagcacca tgtgcggcaa cggcgggcgt tgtctggcgg cctttgctgc gtcgctgggt
    27781 ttcttgaatg aaaaccgttg tcacttcatc gccattgatg gtccgcacga cgccgtgatt
    27841 gtacgcccgg actgggtaga gctgcgcctc ggcgatgttc gggagataga aaaaggggcc
    27901 ggcttttttt gtctcaatac aggttcgccg cattatgtgc gattcgtgga agatgcccgg
    27961 cacttagatg tcaatgccga agggcgcgcg gtgcggtact cggagcgctt tgtcgccgag
    28021 ggcatcaatg tcaattttgt agaagaaacc gcagagggct tgctggtact gacttacgag
    28081 cgtggcgtgg aggcggaaac gctgtcctgc ggcacaggcg tgacggcttc ggcattggca
    28141 tacagcctga aaatgcagca aaaaggagca gtctccgttc ccatcatcac gaaggggggg
    28201 cgtttggaag tgaagtttca ggcgcaggac gagggattca gcgatgtgtg gctctgcgga
    28261 ccggcggtgg aggtgtttga aggtgtgtat tggcagcaat aaaaaagccg cctcgaaaaa
    28321 tcttccgaag cggcttcccg ggctgctctc ctcacgcctc ggcgtggtca tcgagactgc
    28381 gctgcgcttg tatgccctac tgatttattt cagcaccacc gagcctttgc cggccaagta
    28441 gcctttgttg tacacttcca cttcgtattt gcctttgctg aaggcctgcc ccggcgacca
    28501 caccgagcac atcttctgag gcgtaccact gtaattgaac tctttgctca tcgtgtagcg
    28561 aatctgatcg ttgtcggcag ccgtgaaagc gccggaaccc agcccctcta ccgccagcgt
    28621 ctcgcccagc gggctgatga tgcggatgta gtaggtttcc ttgccgtttt cggccacatc
    28681 gttggcagtg gtattgaagc acacttgcaa ttgatcaatg ttgtcggcat agtttttctt
    28741 caccgacttg ccgccgtctc tggttttcag gcccgtaact tctacgccct gcacggcgat
    28801 catggaggcg cggtccacct ttttggccaa tacggtgcgc tcctgttcta cgcgggcttt
    28861 ttcaccagcc agcgcttctt tggcggaagc caattcctga ttggaggcgc gttgggcttc
    28921 cagatcgccg gtcagagttt gtttttcttc agacaactgc ctgttttggt cacccaaggc
    28981 cagattctcc tggcgcaact ggttgatttc ggcgaggtat tgttctacct gcccgttgag
    29041 ttttttgatc tcagaacgcg ccttgtccaa gctgcctttg ccggcgagta actggtcaat
    29101 tttcgctttt tgagaagaca actcttccat ttggctttcg atacgggcat tgagttcgga
    29161 gttggagcct ttcatttctt ccaattgaga aagagcgtca tagtactgct tttccaattc
    29221 ggctttcagt gtttcagatt cgctcagttg ggtggtgagt tctgttgact gacgatcttg
    29281 cttaaatttg ttgacaagca aaaaggcaat gacgcccagt aaggcaacga tgatgaccgc
    29341 ggtgacggca atcagacgtt gacgggagtt atcgggaccg gagctgccca tcggagcgcc
    29401 cggtgtagaa gaagaggtag gaggaatcat ggcgttggaa ttttatttgt taaaaaacgc
    29461 atacggaacg cccccggaac tgcgaaaatt gcccccgaag gcgctgcaaa agtacttttt
    29521 ttgatgcgta tcttaacttt ggggcttact ttttcggaat tgccgtacat ttatacttcc
    29581 attcaacctc acattatgat agaacgcatt gacattgcca acgtcttatt tttagacatc
    29641 gaaaccgttt cggctacggc ctcttacgag gatttggacg aaaactttca ggagctttgg
    29701 tttcacaaaa ctgccggtat tttgcgaaaa ccggaggatg agctcacgga ggaagaagcc
    29761 gacattgcct accggcgcag ggccgccatt tatgctgaat tcggaaagat tatctgtatt
    29821 tctgtgggtt ttgctttcca tgacggcgaa gaaatcaaag tgcggctcaa gtcctttgca
    29881 ggccatgacg aagctgccat tttaaaacag ttcaccgagc tgctcgaaaa atcttacaac
    29941 gacaccaaca acaagtatct gtgcggccac aatatccgcg aattcgacat tccctacatc
    30001 tgccggcgat tggtggtaaa tggcttgccc atgcccgcgc cgctccagct tnnnnnnnnn
    30061 nnnnnnnnnn nnnnnngctg gatacgatgg agttgtggaa atttggcgac aacaagagtt
    30121 tcacttccgt caaattgctc tccgcgctgc tgggttttcc atcgcccaaa gacgatatag
    30181 acggcagcat ggtcggctcc gtatattggg aagagcagga cgtggaccgc atctctttat
    30241 attgcgaaaa agacgtgctg gctaccatgc aactcttcct caagtaccag cgcaagccca
    30301 ttctggaaag ctcgcaggtt atgcatgtga ggtaaactca gccttccgtg cgggtagcca
    30361 gtaccacatg agccaggtgg tgatcgccgt gccaggcata gagagaaacc atttcccaaa
    30421 gnnnnnnnnn ncgttgacgc tcagggtgat agagtgtttt tttccagtcg gacggctgca
    30481 tggattgcaa caagacggtc cagcgctcgt gcagggcggc aatgagttgt gctgatacct
    30541 gtggcggaag ttggcgcgag tcgggcagtt cggcccactg ctcctccaga tacggcttga
    30601 tggcaggcgt tgtttctgtg agagcgagct tgaagcgcac aaatgcgttg atatggctgt
    30661 cggcaatatg atgcaccacc tgccgggccg tccatccgcc gggtctgtag ggcgtatccc
    30721 agttggaggc gtccatttga ctcagcactt cgttgagacg gataggcagg gtgcggatgc
    30781 gttcgatgtg cgcggctaat tcggagggag agaaaatggg attgaaagtg aaacggccaa
    30841 tgggatactg gcgtttttcg ttcacaattg gaggcatgac cttggtttta tggttaagtt
    30901 ggttggtaag gcaagactat ttggaaggca ttttgtaaga cacgttgaag tcgtccagca
    30961 ccatgtccag gatgcgatct ttgccgggta tgtagccttc tttgagattg tggccaaaca
    31021 ttttgccgac agttccccag tagccggctg tccaatttcc tcgcaagtct ttgataataa
    31081 aataggtagg cgttttcaat tcgcgttcta ccattttgat gaggatcatt ccctgcgttt
    31141 tggtcaggtt tttcagcggg tcttcaaaat tgtctttcat gtctttgtag agatgcttgg
    31201 cgtagcgttt ggccttgccc cggcgcatgt cttcggtagc gtcctgcacc tcgtgaaaaa
    31261 ctttgatggc ctccaccgca tacggataca catcggcggc atagcggcgg tagcggcggt
    31321 attttttggc ctcgtcctca ttgtcgaaca tacgcggtga agaaaccgaa atatcctcca
    31381 gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcataacaag
    31561 accttgatgc cggccttttt ggcgggcgca gttcttgtgt tgcagtctat caacaacaaa
    31621 aagcggtact gcgttgagcg caagccgacg gaattgtatc tttacggcca tcacttcacc
    31681 atcattaacg ccatgaagca gtttttcttt ttgaccatcg cttttttact cgcttttcag
    31741 ttcagttttg cacaaaaact cgccgaaacc aagcccgaaa aagcaggcat gagcaccgaa
    31801 cgcctcgacc gcataggcca ggtggtgcaa tcgtatgtgg acagcagcta cattcccggc
    31861 ggcgtggcct tggtggcgcg gcacggcaaa atcgtttatt acaaagcctt tggttttgcc
    31921 gacatcgaaa gccgccgccc gctccaaaga gacgatattt ttcgcattgc ttctcaaacc
    31981 aaagccatta ccagcgtagc cgtgatgatg ttgtatgaag aagggcgctt tttgttggac
    32041 gaccccgttt ccaaatacat ccctgagttt gccaagcccc aggtcatatc gcaatacgat
    32101 gcagccgaca ccacctatac caccgtgccg gccaagcgcg aaatcaccat ccgcgatctg
    32161 ctcacgcaca cttccggcat cggctacgca ggcatcggca cgccggaagc caatgccatt
    32221 tatgccaaaa acaatattcc gtcggggatc ggcacgcccg atcaaaatct cggcgaggcc
    32281 atgcgaagac tcggtgctct gcccctgttt catcagccgg gcgagaaatg gacgtacggc
    32341 ctcaataccg acgtgctggg ctatttggta gaggtgctga gcggtatgcc gcttgatgaa
    32401 ttctttcgcc gccgcatctt ccaacccttg ggcatgaacg acacctggtt ttatctgcct
    32461 gccgacaaac atgcccggct cacaaaattg cataccgaaa acaaggaaaa gaaggccgtt
    32521 ctgttcccca atagcacgct cgatcctgat tttcccaaat tgcagggcaa atttttctcc
    32581 ggcggcgcag ggctgtcttc cactgcctac gactatgccg tttttttaca aatgctgctc
    32641 aatggcggta cttacaacgg cgtccacatt ctcagcccgg cctccgtgcg catgatgacc
    32701 agcaatcaga tcggcgacaa gtacatcggc gccgtcaaga aattcggcct cggtttcggc
    32761 ctcaccaccg aagcagaagc agcccgcgtt ccgccatctg tcggcacctt cgattggggc
    32821 ggcgcttttg ccaccaccta ttgggccgac cctcaggagg gcatcgtagc catgtttttt
    32881 acacaaaaat atccgcactc ttacggcgag ctggccacca aatttcgggt attagtgtat
    32941 caggcagtca aggctggtcg gtaaattaca agcatacttc tacgagcgga tacacgctgc
    33001 aaccttttcg tcataacatt gtcattgtat tggaagcagg atagaaaaac cgaatgataa
    33061 tcggaaatct tgcgtaattt tacacctgat tcaaaatcat tgttatgaaa acgtcttttt
    33121 tgccattggt acttttgttg tcgtttttcg caaccaccca aattcaagcc actgccgaca
    33181 ccggaaacat gccgcaaggc gccgtacata agatgttgga gggcaaatgg caggtcacta
    33241 taaatgccga cgaactgccg caggccgccc gcgaagaaaa tgcggctccc gataatgccg
    33301 attttgccgt catcacttac cgctttgccg acaacggcgc cctcacgcgc agcgtacaaa
    33361 gaggcgctgc tcgctgggaa gaatcgggag aatggcaatt gaccgaggac ggacgctaca
    33421 tctcgttcca cttttccggc aaagcagccg tgcaggccga aatcaaatac atcgaagccg
    33481 acgaaatggt gctggcacag ccctcaggcg caaccgacag caatctgcaa aatgaggtgc
    33541 ggcagttgta tttcaccaaa atgtaggtaa aatgtttact tttacagcct tgccgaaagg
    33601 cttgataaag actgtaaaag catgaaacca ttggccgagc gcatgcgtcc gctcacgctg
    33661 gacgactatc tggggcagca acacttggtg ggaccgggcg ctgtgctgag aaaatccatc
    33721 gaaaccaacc gcctgccttc tttcatcctg tgggggccgc cgggcgtagg caaaaccacg
    33781 ctggcccgca ttgtggcgca tcaattggac cggccttttt ttacgttgag tgctgttcac
    33841 gcaggtgtca aggacgtgcg cgatgccatt gagaaagcca aagggcagcg ttttttcgac
    33901 cgcccctcgc cgctgctgtt cattgatgaa attcaccgat tcagcaaatc gcagcaagac
    33961 tccctcctcg gcgcagtgga ggaaggcgtg gtgacgctga ttggagccac cacggaaaac
    34021 ccctcatttg aagtcattcc ggccctgttg tcgcgctgcc aggtgtatgt tttgcaacct
    34081 ttaggcaaag aagacctcat tcatctcgtt gaccaggctt tggaaaaaga cgaggtgctg
    34141 aaaactaagc ccatcaccgt agaagaatac gatgctctgc tgcgcttcag cggcggcgat
    34201 gcccgcaaac tctacaatgt actggaactc gtctgccatt tccaaaccgg cgaagagccg
    34261 atgctcatca ccgattcatt ggtggcgcag ctcatacagg aaaacttggt gatgtacgac
    34321 aaaggcggcg agcaccacta cgacgtggct tcggccttca tcaagtccat tcgcggcagc
    34381 gaccccaacg cggcggtcta ttggctggcc cgcatgatag agggcggcga ggatgtaaaa
    34441 ttcatagcca ggcgtatgat cattgccgct gcagannnnn nnnnnnnnnn nnnnnnnnnn
    34501 nnnnnnnnnn nnnnnnnnnn nnnnnncagg cggtggggct gcccgaagcg cgcatcattc
    34561 tgtctgaagc tgtgatttat ctcgccactt cgcccaaaag caattcggcc tacgctgcca
    34621 ttggcgcggc gcaggaagaa gtgcgacgca gcggccactt gccggtgccc ctgcatttgc
    34681 gcaatgcgcc cactcatctg atgaaaaaat tggactacgg caaaaactac ctctacgccc
    34741 acgactaccc cggcaatttt gtagaacagg aatttctgcc ggaggagatt tccggcaaaa
    34801 cgcttttcag tccacagtcc aacgcctcgg aggaaaaaat caggcagttt ttggcggcgc
    34861 ggtggaagaa aaggtatggg tattgatact aaattttctc acaaaatccc agcaaatcct
    34921 ccatttccac tgccgaagta aacggatttg tcagcgtcac ccgcagccag gtatgcccgt
    34981 tgatggcggt ttggacgatg taaaaacgcc cgtcttcgag gatgcggcgg cgaatgcgga
    35041 ggttgtgctc gtcagtcgct tcggcagaga gaccgggcgg tgcatggcgg aaacaaacga
    35101 tgttgcactg cggctgcacg gccaattccc actgtggctg cgcctcaatc atggcggcaa
    35161 aaatgcgccc gttttccatc acctgatcca catagttttc aaaaagggtt tcgccgtaag
    35221 tgtagagcaa actgtacgct ttgatgctca tcatcaactt ggtacactca aaactgcgct
    35281 tggccacgtt gtaccattcg cgctcggttt cgccaaaaag atactgcgcc tgctgactga
    35341 acgtgccgta gctgtcggcg tcctgtcgga acacaacagc ggtggtgagg gcgggcgtca
    35401 tcagcatttt gtggtaatcg agggccacgc tgtcggcggt ttcgatgccg gccaatacgg
    35461 cgcggtgctg cctgctgaaa gccagtgccg cgccatgtgc gccgtccaca tgaaaccaga
    35521 gattccgact gtggcaaaac ctgccgatgg cggccaagtc gtcgaaggaa ccggtggagg
    35581 tagaacaggc gctgcccact acggcaatga cctccacgcc ctgctcaagg gcttgccggt
    35641 aaagcatttc gagcaaatcc gtccgcatct ggaagcgctc attgaccggc actttgatga
    35701 tgcctgcatc gccccagccc atgatgcgcg cggcccggtc cacacaataa tgcgcctcgg
    35761 cggagaccat cagcgccagc ggtttttggt ggccgtcttt ccagacatgc gcgccggcgt
    35821 agcgactgcg ggccgctaag agtgcggtga gattagctaa tgtgccgccc gaagtgagga
    35881 atccgccggc ctgccgggca tcgaagccta atacttgcgc tgttttttca atgagcagcc
    35941 gctcgatggc cgttgccggg ccgcccattt cgtacaccgc catgccgttg ttgagcagtc
    36001 cgcccagcaa tcctgccaaa gcggaaagcg gcgcgggcgg gcacacctga tgccccatga
    36061 agcgcggctg gtgcaggtgg atgctgtctt tcaatatttt atgaaaaaaa tcttctgtgc
    36121 tgccgggtac tgcgaggaag tttttccagt gctcgtattg ttcgtcgggc gcccgccagg
    36181 ggatgacggg cagcgagggt cggctgcttt gggcgtcggt caggtagtcg gccagcaggt
    36241 tgatgagggc gtggccttgc tcccggaagt gttcgggatt gtaggcggag tggaggatgt
    36301 ccatgtgaaa tattttggat ggttgtcatc tgccttcggc agtttatcgg ccaacggaga
    36361 gggaaaatct gcctttggca gtttatcggc ttcgccgtaa tctgctgcgc agtttatctg
    36421 ctctttaaac ccgacggtag gcggtaggca gccttcggtg ggcagtactc agcgcgaggg
    36481 aacgcggaac cttgaaccca aaacccggaa cctgaaacct gaaactctga acgcgaatca
    36541 acgcgaaaca cgcgaaatcc cgcgaacgcg aggaaaacgc ggcttgcagc ttgaggccag
    36601 cagccaatag ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36661 nnnngctgaa atcctcgtcc ctgatgcgcg cttccggcca cagcttcatc gccacactct
    36721 gctcgccgcc accgccggaa gaactgctgc tcggactgct gctgaacacc gagccgatct
    36781 ggttgacttc tttggggaag ctctccgaga agttcgtcca ggagttggtc gcattatggc
    36841 cgtaaacacc agaaccaccg taccaggagg gcggttggga aagcagcccg tcgaagtgcg
    36901 cgttccactg tttgagatag ccgaacgcca gagcgaaggg catggttttg tcgtaataga
    36961 gtgggtcttc ctgcataagg cgctcgatga caggccgttc tgcctttttt acaaacaggc
    37021 gaaaaccctc catgcggcga tacgattcat tgccggcagg cgtgcgtttg ttgaactgcg
    37081 tggcaaaaat gaacaacaca atacccgtaa tgatgagagc nnnnnnnnnn nnnnnnnnnn
    37141 nnnnnnnnnn nnnnnnnnnn caagccccag gccatggccg ccgcaccggc aacacccacg
    37201 ccgcagccga gcttttcctg atcggcttgg taccagccct gtgctttgat ccagcttttt
    37261 acctcggtct gtaccgcaga gatgtgcatg taaaatttgt ctttcaaact ttccagatat
    37321 accacatcgc cggtggaaaa gagggcattg aagaactttt tttcaaaggc aaacagttcg
    37381 tcgccggctt ctttgagttt gtaaaaagtg attttatctt ttccaaaaag gccgctcttt
    37441 tcttccattt ccaggcgcag gtagccctga ttggccaagt gcgggatgag gcaaagcacg
    37501 tcgctttgat ccacgctgtg gtccacgaag ccgcccacga ttgcaggaga tacgccttca
    37561 ggcggaaagt attccaccat gatggtttcc ttgcggttgc gcgccctgcg aagcgccgcc
    37621 caggctgccg cgaggaaaaa gaaaggaacg agcagcaggc tgtgttgcaa gcggaaagct
    37681 gtccaatcgt ccattttttt aaaggcatgg cgcggcaatt tcacggcgac ggtcacggcc
    37741 tcgcctgccg aaaactcccg gttggtctgg cccgatatgt aattggccga aacgtccact
    37801 acggcatctt ggccctgagc gccgttgtat cccgtatagt attgcacatc gtttttggca
    37861 aggcggatgc cgccgggcag ttcaatgctg aacgaaaatt ttcgggtgac gacgggccag
    37921 gaaataccca gcaaatccca ataaaactcg gcgtgttctt caaaaaaatt gagcggattg
    37981 agcacccggt agcggatgcg ataggtttgg cggccttcca cccagatgtc tttgtgaccg
    38041 attttgagaa tgagatttcc gttttctttg gaggtggtaa acttgaatcc gtccacctca
    38101 atgtcgcgga tgatgcgggt caccattttg ccgttgatct cgctacggag cggaatctgg
    38161 cgaaaaatac cgtggcgcga agtggtaaat tccacctcaa tgacctcttc gaaatcggcg
    38221 taaccctcgg agctgagttt cacattgaca tcgtagttga cgatgttgaa gaactctgcc
    38281 ttggccggca aaacgagcag acacagggcc gctatgagca agcagttgcg cagtctcatc
    38341 ggcatcagaa ttggacgttg acatttttgg tttcctcctg cgccacctcg aagtaatcaa
    38401 gcgcccggaa tccaaaagca ttggcaatga gattattggg aaaggtttcc acggcgttgt
    38461 tcatgtcgcg agcggaggcg ttgtagtagc ggcgggcgcg ttgaatggat tcctccagtt
    38521 cgctgagctt gttttggagg ttcaaaaact cctggttggc cttgagatca gggtaatttt
    38581 cagacagggc aaagaccgat ttgagcgcgc cggagagggc agaatcggcc gcgccgccgt
    38641 ctttcccgcc ggcactcatg gccgagttgc ggagggtcac aattttctct aaggtgctgc
    38701 tttcgtactg ggtgtagcct ttgatggtgt tcactaagtt gggaatgagg tcatagcgtt
    38761 gtttggtaaa aacgtctatg gatttgaagg cttcttcggc gccgttgcgc aagcgaacga
    38821 gtttgttgta agttcctgcc aggagaaacg ctgcgaggag gaggattcct acaataatga
    38881 gcaaggttga catggtgaat atggttttgg taagtaataa tttgctgcgc agtttatctt
    38941 ggttgttcga tcatggttgg agattttgta cacctcagac tgcgattcaa aagtggcgga
    39001 gtacagcaga tcggacggct tttttataga cgaaagcgcc tctgaacgcc gtccaactcc
    39061 cgctcctaca cccccgccgc aagccttgcc gcctgatcta tggcgcgttt ggcatccaac
    39121 tctccggctt cgagcgctcc tccgatgagg tgtactttca ctccggcact ttctaagggc
    39181 agttgcaatt cccgcagcgg ttcctgtccg gcgcagacga tgacgtggtc cacttcgagc
    39241 aggcgcggct gccctttgac ggtgatgtgc aagccggcat cgtccacttt aacgtattgt
    39301 acctcactga tagactgcac tttgtggcgt ttgaggtcgc tgcggtgaat ccaaccggtg
    39361 gttttgccga gcttctcgcc gggcttgcct gccgagcgct gaagcaggaa aatctcccgc
    39421 tccggcttgg tgggcgctgg tttagtcagc gcgccgcgtt cggcatactg catgtccacg
    39481 ccccagtatt gcataaaaag ttcggcgctt tgcgaggtct cactgtgctc gtgcaacagg
    39541 taagaagcca cgtcgtagcc gatgccgccc gccccgatga tggctacccg gcggcccact
    39601 tcgcggcggt gtttcagcac gtctatataa ctcagcactt taggatgccc gatgccttcg
    39661 atgttcagct ttcggggact gataccggtg gccagcacca actcgtcgaa ttgatattgg
    39721 cggtgcattt ccgcatcgaa gcgggtttgc aggtgcagtt gcacgccggt cagttctatt
    39781 tgttttctga aataccgcag cgtttcgttg aactcttcct tgccgggaat gcgccgcgcc
    39841 atgttgaatt gcccgcctat atcagcctcg ttatcaaaca aatgcacctc gtggccgcgt
    39901 cccgcagcga tggtactgaa ggccaatccg gcaggacccg cgcccaccac gccgatgcgt
    39961 ttgcgttggg tagtagggag gtagttgagt gtggtttcgt tgcaggcgcg cggattgacg
    40021 aggcaggaag ccgttttgcg cttgaaggtg tgatccagac aagcctgatt gcaggcaatg
    40081 caggtgttga tctcgtcggc gcgactttgg gcggcttttt ttacaaattc aggatcggcc
    40141 aaaaacgggc gcgccataga caccatatcg gcatgaccgg aggtcagaat gtgctcggca
    40201 gtttcgggcg tgttgatgcg gttggaggtg atgagcggaa tgttgacctt gcccatgagg
    40261 cgctgcgtga cccaactgaa gccgccgcgc ggcaccatca tggcgatggt gggaatgcgg
    40321 gcttcgtgcc agccgatgcc ggtgttgatg atgctggcgc cggccgcttc gatctcgcgg
    40381 gccagcaatt ccacctcttc ccaggtgctg ccctcagatc aagcatggac aggcggaaaa
    40441 tgatgatgaa attagtccct acggcctcgc gggtgcgccg cacaatctct accgcaaagc
    40501 ggatgcgatt ttcaaacggc ccgccccagc ggtcggtgcg gtggttggtg cgcgcagcaa
    40561 tgaactggtt gatgagatag ccttccgacc ccatgatttc gacaccgtcg tagccggcct
    40621 cctgagctag cagggcgcaa cgcacaaaat ggcggatttg ccgctccacg ccgcttgctg
    40681 tcagcgtcca gggtttgaac ggcgaaatgg gcgactggat gccgctgggc gccacgcaaa
    40741 gcggatggta gccgtagcgg cccgcgtgca gaatttgcat acagattttg ccgcccttgg
    40801 catgtacggc tttgggaatg acggcgtgat gccgggcttc ggcgcgggtt gtcagtttgc
    40861 cggcaaaggg cgccagccgg cgcggttggg cgcaatgcct cccgtgacga tgagtccgac
    40921 gccgccggcg gcgcgttcgg cgtaaaaagc ggccattttt tccataccgc cggggctttc
    40981 ttccaatccg gtgtgcatag accccatcag tacccggttg ggcagttggg tgaagcccaa
    41041 atccaagggc tgcaggaggt gtgggtattt cgtgtccatc atggccgaaa ggtaaggaaa
    41101 atctgccgaa ggcggtttat cataagtaat ctgctgcgca gtttatcggc ctcactgtac
    41161 ccgctgcacc gacttcgcct tcggccccac aaacacaatc gcatacgccg ccagattcgg
    41221 gatgcgacgc aggtcgtcgg tttgcaggta ggcttccaat tgcgcgcgcg cttcttcggc
    41281 cttggcggtg agttgtttag actctgattt tttcagatat ttcagttcaa aagcaaaact
    41341 ccagttcacc ggaatctgcg gcaacttggc acacagtatg tccacgaaac ggcgctcggt
    41401 ttcgtgttcg gttcttacca aatacgcttg tgaggtattg agcagcgccg cccagatgac
    41461 ttgcaggtgg ctttcgctga agtgctgcac atcgcggccc gacagccggg agagaatgcg
    41521 ctccacaatt tcgatcatgg gctgcaattc gttgtccatg ctcagtgtgt aaaacgcgtc
    41581 gttgatgtcg gtgaaaaccg tctctttcag ttcggcccgg cggcgcagtg tatccaaaaa
    41641 gtagctgtaa tacagccctt taatgacaaa attgggaatg gtaaaattcc aaaacgaacc
    41701 cgcccgattg cgcaccgtca gcacgccgag gtaaaaaagc aggctggtat aatcgtctcg
    41761 cacccaatcc cggtcgaagc tgaacatgcc cgtcaggttg gcccgcacga agtctttttc
    41821 taatgcgtct tccagaatgg agaggctcat ttcctcgccg cccacgccca gcagacgccg
    41881 gattttgccg tagtcgctgg cgatgttgtc gtccaagagt ttttcggggt attttttgtg
    41941 gcgttgccag gcgttggcaa agtaaatgag catgttggga ttgtacaggt gcgtttcggc
    42001 gtcggggtgg aagaggtagc cgtcgtacca gttgcggagg tcggcgatca tagcggatat
    42061 ttcagattgc gacactccgg caaatgccag caaatccacg gcttcctcct ctgtgaatcc
    42121 catcatgttg tggagttcca actccgtccc tattttttca gcaatattaa acccgctggt
    42181 gaggctgtcc aaagtaattg ggcttacgcc tgtggcaaac atgcgctcta ccaaaccgtt
    42241 gcgactttct tttttaatag tctcaaaaaa cttgcgtacg aagccgttgc gtcccacaat
    42301 ttctttaaaa tcctccagat gaaaggcaat cagttcgttg gcaaagtggt cgtattcgtc
    42361 tatgaggatg aggagcttac ggtcgggcca aaggcggtaa gcgaagttga aacagcgctg
    42421 gataatatct gccggcaaat tctcttcctc tacctgtcgg cgatcttcgg cagtgatttg
    42481 tcccttgccg taattgacca taaagcgctc tacccctgtt tttaccgcca gattaaagcc
    42541 gtcaagcacc agttcccggt tatcggtaga tatcccgctg aaatctaatg tcagtaccaa
    42601 ataactgttc gccagcggcg tgggatgttg gccaatgtaa tacttgccga aaatgcggcc
    42661 gaaatcatct ttgtgctgta atccgtagta gtactccagc atggaaatga agaggctctt
    42721 gccgaatttg cggggccgca ggaaaaagag gtagcgatta gcttgttgct ccaacgtttc
    42781 gatgtactgc gtgcggtcca catagtaaaa ctcggtggcc acattgagga agttgctctg
    42841 tccgtacggt agtttcatgg ggcgaagata gggaaaaatc tgccgaaggc ggtttatcat
    42901 aagtaatctg cttcgcagtt catcgctaat ggagagggca aatctgtctg cggcagttta
    42961 tcataagtaa tctgctgccc gcgccttgcg cggtgcgcag tttatcttgc taatggagag
    43021 gggcctcggt acgcatgtat ttttttgagg ctccggacaa tgaaataaaa atcacctcag
    43081 cctgccccgc cggccttcaa gcgctcgcgc atatccgcca cgaggcgttt ggcggcctcc
    43141 tcgcgttctg cttcgctcca ggcggtattg gtgcgaatca gttccagctc ttcggtcagt
    43201 tggcgggtag caatttctgt atcctgaagc catgagctga attgctcttc gttgccgctg
    43261 ccccaataac ccgactcgtc ctgtacttca aagaccaaaa agtatttgtc ggccaaataa
    43321 tggaaaagtt tgcacagtgc gatatgtgtt tcggggccat cgttgccggt tttgacgcgc
    43381 tgccagggga agtcttcatc gtttccgata aatagcggat tgacgagtgt aaaaaaagac
    43441 tggagcgtgc cgtcgggctg gaacgtaagc catgccgtct ctgtgtcgcc gatgttgagc
    43501 gtgatgccct tgagtggagc attgccggta aagttcatgg ccgttccagt gaaagccata
    43561 tctaatgtgg aaggcaggga ccagtcttca tcccacactt tgtaattcca ggcattggct
    43621 gtgcagatgt cctgcacttc ctgaatcagt tgatatactt ctgagggctg cctgagcctg
    43681 ccccggtagc ggagtttgat gtacatattg gcaatccctt tacttcggaa tcctcacctc
    43741 ataagtcttc ccggccttat tggtcagttt cggagcgatg atccaggggt tgtagaggcg
    43801 caacatgcgg tacgagatgc cctgttcctg cgcgaaatcg ggccagtgag ccaccgggcc
    43861 tttcacatcc accactttgt attcgtcgag cggcgagtac ttgtcttttt cttccaacac
    43921 aaaaccgaaa gcctgcggat tggccaatat ttccttgatg gccacgaggc ggaacacata
    43981 gcggcnnnnn nnnnngttga gctggaggtc gtagaaagtt ttgccgcgtt gggcttcttt
    44041 ttcgcggccg aggcgtggcc cgccgatgtt gtaggcggcg gcggtcaggg tccagttgcc
    44101 gaattggcgg tggtagtcgc gtagcagttt gcaggccgct gcggtagctt tttcgggatg
    44161 gtagcgctcg tccacttcgt cgttcacttc cagtccgtat tgttgggcgg tgggcgccat
    44221 gaactgccag atgccgcgcg cgccggcagg agagacggcg ttgcggaggt tgctttctgc
    44281 cacagcggta tatttaaaat cgtcgggaat gccgttttct ttcagcnnnn nnnnnnnnnn
    44341 nnnnnnnnga agtaacggta cattgccttg atattcagca gtgtagtgcc gtgcgtatag
    44401 gcattgacgg tcagttcgcg atccaagcgt tcgcgcacgt cgaagttgtt catcggcagg
    44461 gcttcgccgg caaaatcgaa atctcggtcc aatgaaaccg gctgtatgtt ttggggcagt
    44521 tctttgaaag cgctgaccaa tttttttacg ccgtccggag cattttcacc gccggaatag
    44581 gccaccaaca agaccgccgc caacagcagg gtgacggcaa gggaataaac atagagcatt
    44641 ttttgcatgg ctgtcctttt acttttgtag cggggcaaaa atagaaaaac cggcgtaagc
    44701 gctcgctttt ttgtaccttt gtccacctta aagtcaacaa tcatgacagc agacaatctt
    44761 ttgcagtttc tccggcagcc gcaagacttg cagcgggttt cgtatcagga gttgaaaact
    44821 ctggcattgg agtacccctt ttgcccgcat gtacaccagt tgttgctgtt caaaagcagg
    44881 ctttcgggtc acaaggatta cgccgccgat ctgagccgca ccgccgctca cagcgttgac
    44941 cgccgctacc tgcgcagccg tttggaggag ttggatgcag cattggccgc cgttcaggag
    45001 gaggtttttg aactgaaacc cgtggcggaa attgagcagc aattggccgt aagaaaagaa
    45061 cccaacgctg tggaactcag cccgtctgcg ctgcgccccc cgcgccacag cgcggctgcc
    45121 gacgaccgtc ccgatcaggc cgccgccccc gaagccatac ccgctccgtc gccggtgccg
    45181 cgccccaaac gccgcatgcc cgctggccat actaccgacg acctgccggg cgatgtgccg
    45241 cagcctttgc aaaaagatgc tttccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45481 nnnnnnnttt gctggcccgg caggggcacg ccgacaaggc catagaaatg tatgaaagat
    45541 tgcgcttgct ttttccagag aaaagcgcgt actttgcggc ccgaatcgaa aatttgaaaa
    45601 aataaaaggt atctgatatg tccatcctga ctgttttcat cgcatttata ggcattttgc
    45661 tcatgatcgc cgtgttgatc caaaacccca aaggcggggg cgtggactct acgttcggcg
    45721 gcgcgcaggc caaccagatg tttggtgctg ccaaatccag cgattttatt gaaaaagcaa
    45781 cctggggcct cgccgcagca ttgctggtat tgtgcctcgt tgctgctgtg ctcgtaggcg
    45841 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgcctttcg tgtttatatg
    45961 tattgctttg acgacattgc ctatctgcaa caaggctccg acaagcagcg ccgcgtatgg
    46021 gaattgctta cccggcattg tatttttgaa aaattagccc ttttcgaccc gcttctcacc
    46081 ggtacgattc ccattggaat agacacgccc gacagcgact tagacatcat ctgccattgc
    46141 cccgatgtcc cgcactttga atccgttttg cggagtcttt tcggtacgga gcaacacttt
    46201 cgattgcact gtaaagtcat cggaggccgt acttctgtgg tgtgttcctt tgaaaaagag
    46261 ggttttatga tagaaatttt cggccaagac cagcccnnnn nnnnnnnnnn nnnnnnnnnn
    46321 cggcttcaaa acgcctatcg ccatttgctc attgaggcgc ggctcctgga agaacgcggc
    46381 ccggaattcc ggcagcaaat cattgaactc aaacagcagg gcgtcaaaac ggagccggct
    46441 ttcgcccgat tgctgggctt ggagggcgat gcttatgagg cgctgttgag gttagaatct
    46501 taaatcaacc cctcccgaat caaatcgtgc agatgcacca tgccgaggta gcgcttgttt
    46561 tcggctacga tcagctgagt gatactgcgc aggcgcatca actcgaaagc ctcggtagcc
    46621 tgcatatcgg gggaaaccga gatgggcgaa ggcgtcatga tgtcggcagc cagtatgtgg
    46681 tcgaggccgt tggtgctgcg ttcgatcatg cggcgcaggt cgccgtcggt gatgatgccc
    46741 agcaattggt cgttttcgtc ggtgacggca gtggcgccga ggcgtttgga ggtcatttcg
    46801 atgatgacgg cgcggatgga gtcagcgggt ctgacggcgg gtttttcgtt ggcagggtag
    46861 agatcgctca tgcgcaggta gagttgtttg cccagcgcgc cgccggggtg gaacaaggcg
    46921 aagtcgtcgg ggctgaagcc gcgcagagcc agcagtgcgg tggccagagc gtcgcccagc
    46981 gccatttgag cggtggtgct ggcggtgggc gccaggttgt tgggatcggc ttcgcgcgag
    47041 acgggcgtgt gcagcacata ttgcgattgt tggcccagaa aggaatgccg ttgggccgtc
    47101 atgccgagca ggaggttgcc gcggcgtttg accaaaggca ccagcgcttt gatttcgggg
    47161 gtttcgccgc ttttcgagag gcaaagcacc acgtcgtcgg gcagcatcat ccccagatcg
    47221 ccgtggatgg cgtcggcgcc gtgcatgaaa atggccggcg tgcctgtgga gttgagggtg
    47281 gccactattt tttgcgctac gatggcgctc ttgccgatgc cggtgacgat gagccgcccc
    47341 cgcgatgcaa aaacagcctc gacggcagct acaaaatctt catccaccga gggcaacaac
    47401 tgttgcagcg tctcaatttc tatggaaatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47581 nnnnnnnnnn nnnnccctga ttcctgcccg caaatgtcgg ctagtccaca tcgtcgggca
    47641 aatccgggtg cgcttatttt tttttcgcag ggaactgcca aacggaacat atcaaaacag
    47701 atagaagaac catgacactg accaatgaat taacattgca cgacgctttg cagcggtatt
    47761 tcggcttcga caacttcaaa ggcatgcagg agcagattat cgaaagcctt cttcgcggtg
    47821 aagatacgtt tgtcattatg cccaccggcg gcggaaaatc gctttgctac cagttgcccg
    47881 ccctgatgct cgacggcacc gccatcatca tctcgccgct catcgcgctg atgaaaaatc
    47941 aggtggactc catacggggc tacagccaaa gcgacgacgt ggcgcacttc ctcaactcct
    48001 cgctgaccaa agcccagatg aaattggtaa aacaagacat cagcgacggc aaaaccaagc
    48061 tgctcttcat agcgcccgaa accctcacca aagacgaaaa tattgagttt ttccaacagg
    48121 tggatgtcag ctttgtggcc gtggacgaag cgcactgtat ctccgaatgg ggccacgatt
    48181 tcaggccgga ataccgccgc atccgctcca tgctcgacgc catcggcaaa gaaatcccca
    48241 tcatagcgct caccgccacc gccaccccga aggtgcagtc cgacatcgtg aagaacctca
    48301 atatgaggac gcacaacacc ttcatttctt cgttcaaccg cgaaaacctg tactacgaag
    48361 tgcggcccaa gggcaaaccc gaacagaccc tcaaacagat tgtgcaggtc atcaaaacca
    48421 tgccggggca gtcgggcatc atttatgttc aaagccgaaa agcagccgaa gacattgcta
    48481 aaacattggt ggtcaatggc ataaaagccg cgccctatca cgccggcctc gacgccaaaa
    48541 cccgcaccca aacgcaggac gacttcctga tggaggaaat tgacgtcatc gtggccacca
    48601 tcgccttcgg catgggcatc gacaagccgg acgtgcgctt cgtcatccac ttcgatattc
    48661 ccaaaagcat cgaaaattac tatcaggaaa cgggccgcgc cggccgcgac ggactcgaag
    48721 ggcgctgcat cgcgttttat gcttacaaag acatcctcaa actcgaaaaa tttctgcgcg
    48781 acaagcccgt tgccgaacgc gaaatgggcg ctcaactcat gcaggaagtc atggcctact
    48841 ccgaaaccac cgcctgccgc cgccgattcc tgctgcacta cttcggcgag gaatacgacg
    48901 acgcgcaatg caacgacatg tgcgacaact gccgccaccc caaggcccgc atcgaggtga
    48961 aaaaagaaat ggtgatggcc ctgcgttcgg tgaaggaatt ggacgaaaac ttcgccatca
    49021 agcccatcat tgactttgtg atcggacacg aaaccaagga aatcaaggac tttgggtttg
    49081 ataaaaaaga actctttggc gccggcaaag accatgatga caacttctgg agttcggtgc
    49141 tgcgccaggc catgctcaac ggcttcatca acaaagacat cgaaagctac ggcctgctga
    49201 aaatgaccga aaaaggccat cattttatta aaaacccgca gcctttccag attccgctca
    49261 accacgacta cgacaaagag ctgcccgaaa gcgacgagcc gnnnnnnnnn nnnnnnnnnn
    49321 nnnnnnnnnn nnnnnnnnnn nnnnnnnaag acctgcgccg ccgcgaggcc aaacgcctca
    49381 acgtgccgcc ttacgtcatc ttccaggacc cctcgctcga agacatggcc attcagtacc
    49441 cggtctcgat ggaagacatg gccaaggtga gcggcgtgag catcggcaag gctacgcgct
    49501 acggcaaagc ttttgtagaa ctgattcgca agtatgtgga ggagaacgag atagagcggc
    49561 ccagcgaggt ggtggtcaaa caaatggcca ataaatcgaa ggccaaagtc aacatcattc
    49621 aaaacataga ccgcaaaatc agcctcgacg acgtggcccg cgctaccggc ctgaacatgg
    49681 acgaactgat ggacgaactc gacgccatcg tggagtcggg taccaaggtc aatattgact
    49741 actatctgga agacgccttg gacgaagccg tgcgggaaga catctacgat tacttcatgc
    49801 aatccgatac cgacgacatc aaagcggcct acaaagacct gcgcgaagac gaggtgacga
    49861 tggaggaaat ccggttggtg cgcatcaagt ttttgtcgga gatggcgaat tgaagcgggg
    49921 cagggcaacc atttttactt tgcaaatgta caataggtgt aaattcatcc gacatgaatc
    49981 cgcttttaga gagaatatcc gtcaatccgc aggtgtgttt cggcaaaccc tgcatcaagg
    50041 gcactcgtat ttgggtgtct ttgatcttgg attttttggc aacaggtact ggaattgagg
    50101 agattttgca acattacccc caattgcaac gagaagacgt gctggcttgc atcgcttatg
    50161 gcgcggccat gtcgcacgaa catttcgtag ccattgacat cgctgcggct tgatatgctt
    50221 aggtttaaac ttgacgaaaa tttcagtccc tcattaactg aattattcaa ggcacacaac
    50281 tacgatgccg aatcagtctt agacgaacaa ctatcaggtg ctccagattc agatatatat
    50341 gaggtttgca ggcaggaaaa tcgctgcctg ataacatttg acttagattt cgccaatata
    50401 ctccgctttc ccgctgaatc aactcaagga attgttgtta ttcgcacgaa ccagatcatc
    50461 acgctggaag tcatgaaaca aatgctggag atactcctcc aactttttga atcatcttcc
    50521 cccacgggct gcctgtggat attggaaccc catcaactca gaataagaaa gccaaaagag
    50581 tgaaagtcgg cctcgctcac cttgcatctc tccagttgag gtacttttac cgaatacaga
    50641 ccgtagttat gatacagcta taccggaaaa tttcggagtt ttatcaaaat ttcaaccgtc
    50701 acgctacctc aacatgaaat acctcctcct cctctccctc ctcccgctgc tcctttccgc
    50761 gcagccggct acccggaaac aacagccgcc ttttcaggaa ttccgcttgg acaatggcct
    50821 cagagtcatg gtggtacaaa acaaaaagct gcccgatctg cactttcact ttttcaccca
    50881 actgcccgta gcccccgaag aaaacttagt cggatacgag gaactcaccg ccgtcatgca
    50941 ggccataggg ccggcgtcca aagcaatgtg ggacgagcag gttgcccact ggggcgctct
    51001 gtttactgcc ggccccgatg gcatcatggc agtttgccaa gcccggtatc gggaacagat
    51061 gatggcgttt atggccgaca tggtgctgcg gcccgccttt gatcctgttg attttgannn
    51121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cccgccaccg
    51181 tagccgccaa tgtggccgcc gtgctcgcct tcggaaaaac acacccttac ggagaattgc
    51241 ccacgccgca aaccatccgc aacatagagc tgaagcactg ccgcacatac taccacgctt
    51301 tttatcgccc cgaaatgtcc tgtttggtca tcatgggcga tgttcggctg cccgatgccc
    51361 ggaaattagt ggagtcacat ttttccaatt ggcaacaggg cggcattctc acccagtctt
    51421 tcgagctgcc tcccctaccc cgctccacac aaatagccta cgttgcgctc cccgatgccg
    51481 atacnnnnnn nnnnnnnnnn nnnncgtacc ccgtgcaact ccggcccggc gatcccgatt
    51541 tttggaaaac cttgctgctc aataccgttc tgctccagcg tctcaacgac aaggccggaa
    51601 agtttgccac cgattttcaa ttcggtatca cacccgaccg agaaatggcg ctcttcgtag
    51661 cagcggccaa agtcccctcc cggcatagcg acagcctcgt aggcctgctt ttgaacgaaa
    51721 tagaagtctt gcgccgcgaa ccgctttctg aaactgagat ggagcgagcc agacaactgc
    51781 tacacgatac ttggaaatgg acttggggac ggccattccg cgtcagtcgt ttcgccatag
    51841 acgtggcgcg gtatcgcctt ccggcagatt tttttcatcg ttggagggag cagatttcta
    51901 aaacaacgcc gcagcaactc catgccgccg cccgaaaata cctgcccgaa gaacaggcgc
    51961 acatcctggt ggcaggccaa cgggaagacg ccgccgccct cacccgtttc gatggagacg
    52021 gcagggtagt tttttatgat gtgtacggta cccccataga cgacaacaac ctcctgatgc
    52081 cggagggctt agacgcaaag ggcatcattg aggggtacat acaggccgta ggcgggcagg
    52141 aacgcttggc ctccatttcc gacctgacgc tgcacatgag cgcgcaggtg caagggcagg
    52201 aagtgaaaat gacgatacaa aaaaagggcc ccggcaaggc gcttgtcgtc tcctcggtgc
    52261 agggcaacgt gctgacaaag attgccttcg acggagcgca ggcctcggtg tatgcgatgg
    52321 gcgaaaaaca agcattggac gatcaggcaa cggcttccat ccaacggcag gcttggctgt
    52381 tcccggaaat cgagtacctt cgcagcggtt ctttgtcgtt tggaggcttg gaggatataa
    52441 atggccgaaa agcctggcgc gtggatgtag tgacgcaaaa agggtacgcc tgttcagagt
    52501 attacgatgt ggagacggcg ctgaagttgc gttcggtgac catcgaggcc ggcatgaggg
    52561 tggtgaataa ctactccgat taccgggagc agcagggtat tttgcttcct tttgaaattt
    52621 tgacgacttt tgccggcgaa cagcagccgc ccatgttgat gcgggtgagt gaggcaaagt
    52681 tgaacacggg attgagcgac agtatttttt tcatcagata acctttttaa aatcaaaacc
    52741 ttatgcaaga agatgatctg attctggacg aacctttgtt tgaagaccag gagtggaaac
    52801 ccgctcacgc ttccaaaggc caacgctttg tcaactacct gctcgatcag gtggccatga
    52861 caggcatgat gagtgctttt tttattttca gcggccttgt agaggtcttt gaagatagta
    52921 aattaggcaa ttggatcatt ggagccatct ctgcgctcat ttattacacc ctcatggaga
    52981 gctttctgca aggcaaatca ctcggcaaat acgtcaccaa aacgcgggtc gttacgcacg
    53041 agggctacca gccggaactt tccaccattt tggtcagatc gctgagccgc gtagtgcctt
    53101 ttgatgcgct gtcgtttttg ttcaaagaac ccggcggctg gcacgatgag tggtccaaaa
    53161 cttatgtggt agaagatcgc tgcctgcggg tcaaccgctc tcctgtcgaa actgtataaa
    53221 gtatcatgat acaagttttt gtaaccggcg gtacttttga caaagagtac aactatatta
    53281 acggagcgct ctttttcaaa gacacccacc tgccggagat gttttcccgt gggcgttgca
    53341 ccgtagaggt ggatgtcaaa acgctcatga tggtggacag cttggaaatg accgacgccg
    53401 accgcgacat catcatccac aattgccgcc gctgcacgca ccaacgcatc atcctcacgc
    53461 acggcaccga tcgcatggtg gaaactgccc ggcacctggc tcaggcgggc atcgaagaca
    53521 aaaccattgt actcaccggg gccatgattc cttacgcttt cggcacttct tccgacggtt
    53581 ttttcaatct cggcagcgcg ctggcttttg cgcaaacact gccgccgggc gtgtatgtgg
    53641 tgatgaacgg gcgctatttc gagtgggatc atgtgcgcaa aaacctgaac accggcaatt
    53701 ttgaggaggt gtcctaacga atgaaagtaa gttaaacctc ttcttccgcc caatccgaca
    53761 cggccttttt cacactatcg aaattgatgc ctataccaat tacgggttta ttttctaatc
    53821 gtagggcagc ggcatagtcc ttgcgccgaa tttgcgagag ggctgcctca gcgctctggt
    53881 ctattttgaa ctccagcaaa taaatatgcg tgggggtttg caccacagca tccattctgc
    53941 cgtcggaggt atgcacctcg ctttgaatga acaggccgag gtagcgaaaa tgcaagtgta
    54001 ccaaggcatg ataaaaatgc tcgccttttt catccaacaa atggctgggt actcctttga
    54061 gcatggagtt gatgacggtg actacccgcg caatgtcatt ggcgaaaaaa gcctcctcca
    54121 attgctgtac aggaactacc gtttccggcg cggcgcgatg aagtagtacc gatagcagat
    54181 gcccggtcag ggcttgctcc acttcccggt tggggtagtc aagggtataa ataccgcgaa
    54241 tatggtctat atttttgatg gtcaggtagc cggtttggaa gagcagagaa cgcgtttcga
    54301 ggttgtctaa agaataactt tcaaaaatca gaccactgac tttcaggtca ttaagattaa
    54361 aataacccga ttctttgatt ttctgaataa ggaacgtcgg cgtaccggtt ttgaaccagt
    54421 agtcttgaaa agacccggac gcaaagaagt tgagcaccga aaaggggtta tatacatggt
    54481 ctcgtccgtt ccaggtgtag ccgtcgtacc atgtcttgat ttgagccagc aactcttccg
    54541 tcgtaacgcc cggcatttcc tccaaggtgt attgcagcca ctcctgaaaa gtattttcca
    54601 attccggctg cgtataaccc gtcagagtgg catggcgcgg gttgagcgtg atgtcgttta
    54661 aattgttgag atcgctgaaa atactgacct tgctnnnnnn nnnnnnnnnn nnnnnnnnnn
    54721 nnnnnnnnaa ctccagatac gggtcggcgc ttttgatgat gctgtaaaaa ctcttcaaaa
    54781 tgcgctgatg ctcaaaggcg gtgggcaact ggtctttttc taaataatca atcagtggct
    54841 tgtcgtactc gtctatgagc aatacgactt tgcctttttt ttcggacagt tcgcgtatca
    54901 actccctgaa cttctggtcg taggtcgcct ccttgagcga aaggccatac atcgccgcct
    54961 gctcctctaa tgttttgtgc aaagcagact ccaaaccgcc cgtattgtac ccgatctcgt
    55021 tgaactgaat atgcactacg gggtgtactt tcgtccaatt ccattgatnn nnnnnnnnnn
    55081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55141 nnnaatttcc cgaatggtgg acagcgtgag cgacttgccg aaacgccgcg ggcgagagag
    55201 gaaaaagtat tgccccgagg aaataagctg gtatatatgc cgggtcttgt ccacatagag
    55261 gcagtcgtcc tctctgatct tgcggaaagt ctgtatgccg atgggtaaat ttttcatagc
    55321 gcggtaatgg aatatgggca aagatacgaa aagaaaaaac ttgcgcgaaa cccaactact
    55381 taggccccct ctcgctgccc ccggaaaaac gcccgcactt cttcccagtt gttcacgcgt
    55441 atgaaatcgg tctctaaggc attgtgcgaa gccgtgaaca aaagcggagt gccctcaaac
    55501 gagcgcaggt ttttgacgtg gtcgtctatc atgtaatcgc cgcgaagtgc gcgtttgtcg
    55561 ccacaaaaaa tgacgttctt ccaggggata aaagaaaaat gctcctccag ccagtcgtac
    55621 ttctcggtca gcgagtgcct gaattccata gccgccgaat tgatgaatac ttcgtaatgg
    55681 tccatcaact cccgaatgac ctcctggctg cccggcatta caggtaaatc ggagaaaaag
    55741 cctttgtcgt gtaaataccc ccgaacgcga tctgcgcccg gcaattcgta aatctttttg
    55801 ccccaaaact ccatccggtc ggggcggcga ccgaactcgc gttcaaaaat gtccaaaaac
    55861 ttgggataga cgtcggcgat gacctcgtcc atgtcaatca atattctttt catataatat
    55921 ggttatttat acagcttcaa gctgttagct tcgagctacg agcttatttc tccaaaattg
    55981 aaataaaatt cttcttcaaa gtacattttg gaatttacct aaatggacaa tgattctttt
    56041 tcattaagtt atcaagctcg tggctcgcag ctcgtggctt ttggaacgat gctgacattc
    56101 tgagctatta cttcccttta ccctgcaaca acaccagcaa ggtgtaaaac atgatcttga
    56161 aatcgagtgc cagcgacatg ttttcgatgt agagaatgtc gaatttcatg cgctgcacca
    56221 tttggtccac gttcgatgcg tagccgtatt tcaccatgcc ccaggaggtg atgccggggc
    56281 gcaccttgag caaatgcttg taatgcggtg ctttttgcaa aatcagatca atgtaatatt
    56341 ggcgttcggg gcgcggcccc accagcgaca tatcgccgat gagtacattg aaaaattgcg
    56401 gcagttcgtc taatctccat ttgcgcatca cggcgcccca gggcgtgcag cgcgggtcgc
    56461 cgtcgtgaga cagcctcggc ccctcggctt cggcgtccaa tgacatggag cggaatttga
    56521 aaatcaggaa aggctttccg ttgatgccca cgcgttcctg tttgaaaaaa atagggccgg
    56581 gcgaagacca gcgcacccgc agggcaatgt agaggtagag cggagagagt aagagcaaaa
    56641 acacagatga ggccgccacg tcaatcaggc gcttgataag acgctgccag cgagccatca
    56701 gtccgcgctt gatctcgatg agtacggcgc cgtacaagtg gttcattttc accgttccca
    56761 acagaatgtc gtacatgtcg ggaatgattt ttaccaaaat gcgctcgtca aaatcaaaga
    56821 ggatgttgag gatttcactg agccggttgt gctccgaggt ctctatggnn nnnnnnnnnn
    56881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgcttt tgccgttggt atcaatgaat
    57001 ccgatgaatt tgtagcctaa tcctttttcc cttccggtaa tgtcttcaaa gaggtcaagt
    57061 gcattttgat tgcctccgat gaggagtgtg ttgtaagtga tttcacccga cttgagacgg
    57121 cggctggcgc gcgtgagcag gatcatacgc accgtgcctg tgaggctgaa atgcagcaga
    57181 aaaagggtaa aaaatgagtt gtagtaggta gtatagttgg taacgaaatc gtctaaaatg
    57241 agggtaaaga acaaaaacaa aaccccaaaa aaactgagaa aaagcgtacg ggtaagcgtc
    57301 gcgagccttg acattcggta gatgtcgttg tactgatcga aaataccgta aaacagcagc
    57361 cagcccaatg gaatgatgag gatgccgtac aggaaattgg ggtcattcag ggtagccgtt
    57421 cctaaagaag cgccctccaa atgtttgcga tacataaaga agcaagccca tgacagcatg
    57481 gcggtcacaa agtcggcagc ctggtacatc aaggtgtgcc gttgtcgttt gcgtgtcata
    57541 tcatgtcgtt gtcccatcat acgcgggcga gatagttgcg gtgcggaagc tcggttttgc
    57601 cttgctgaac gaagggttgg ctttcggagt cctccttctc tcaggcaatc gctttacctc
    57661 cttgtcaaaa atgcaccagt ttcacattgt caatccacac tttggccact tttttctccg
    57721 gcacgccgtt ttgcacggga atggccgcct gcaaaacgat ttgatattgc ggaaagcggc
    57781 tattgaatat cgtctggctg agattgaaat aaattttgtt ccagtctgct ctggccctga
    57841 acccggactc atacaaggta gtgccggcga tggggcctcc tgtttcaaag cctaccaggc
    57901 ccaccagtac atccacatcc gatttgtaat tcatctccag atacacatag ggagagctac
    57961 tgttcagccc gctcagcagc gggagcgcca ccacttcggc caccgtattc atcgagtcca
    58021 gcacaaacac accggaagcc tcgccttcaa aaggcatttc ggcactcctt tgtactgcct
    58081 gcgctgtagt tccagtgcgt accgtgcgaa aaatgtgttg tggcccttca aaattttcaa
    58141 tgaaggcaaa cttcgtttcg ggcagatagc tcgtcattgg acgaatgatc tgcgtttcat
    58201 tggcgcccag attcacggtt accgtcaccg gagcgtaaaa tggataaatt tcgggcagtt
    58261 ctccaatacc gttgtctttg atgcctgcct gcaaagtcaa aggttgaggg cctgttccca
    58321 aaacggggaa agctgtgcca aaccgatagg cgcctaaaaa atcgttgccg gcagataccc
    58381 acacctcgct gatcttggac gaagcgctgc cctggttggc cgtcgtagtg atctgaaaag
    58441 gctctatgga aacgtatccg ggaatcggct cttccggatt gatgagatcg caccctgtaa
    58501 gagtggccag tccccatatc agcaaacttg tttgcaaaaa aaacctcata ctgcgatttg
    58561 tgttatgaaa accagatcag gaagtaattc tgatatcgcc tcccgattta tcaatattta
    58621 atcctgccgc tgcctgcctt cgctccatat cggcaatgat gcgccaggcc aagcggagcg
    58681 catcgtaccc gtcttcaatg ctcactttgg ggcggctctg ttgcaggatg ctctctgcaa
    58741 aactttcgag atggcgttca ctggttcaat ggggggcgtt tgtatatgca ccacctttgt
    58801 acctgtggct gtcggcattt ccatcacagg aatgccctgt gccggcgagg cgtcgtcttt
    58861 atcgcggagt tggataactt gtgcttgttt ttccaacaaa tcaaggctga tgtaggcatc
    58921 gcgctggaag agccgtacct ttctcatttg tttcaaagat atgcggctgg ctgtgagatt
    58981 ggccacgcag ccgttgccaa actctatgcg tgcgttggct atgtcgggcg tatcgctcac
    59041 cacggctact ccgcccgcac tcacggctgt taccggcgct tgtaccaagc tgagaatgat
    59101 gtcaatatca tggatcatca ggtccaacac taccgacaca tcagcgccgc gcggattgaa
    59161 ctgcgccaaa cgatgggcct caataaacat gggatgcaat ggcacgtctc ccaaagccag
    59221 caatgccgga ttgaaacgct ctacatgccc cacctgtacc ttcagccctg ccgcctggct
    59281 caggcgaacc aattcttccg cttctgccaa agtgtgggtc agcggttttt ctataaaaat
    59341 atggcgtcct ttggcaatgg cggctttcgc caaagcaaaa tgagtgggcg tgggcgtcac
    59401 gatgtccacc acatccaccg catccagcaa tgcctctatg gtttcaaagc gatgtgcgcc
    59461 gtaagtttcc tgtgcttgaa gagctgttgc gtcgtcgggg tcgtaaaaac cagccaactc
    59521 ccatacgtcc gatgccaaac cgatacattt gaggtgaatt ttgcccagat acccggcgcc
    59581 gaaaaccccg atttttaaca taacgtctgt tttacccggt aaaggtaagc caaaatggtt
    59641 acacccaaat accaacgcgg gtctttactt tgtggtattc ttacagagct ggttggtcgt
    59701 acccgcgacg gaaacaatga atcagggaat ataagcaggg cgaacaaact gaatctcaac
    59761 gataatacat cagcacaccg cccaacaggg taatcagtgc aatgagcacc gaagccatgc
    59821 gcaccgaaaa gcgaatgtat ttcacaaagt tgagaatatc ggttttagct tcaggataca
    59881 agggcaggat ttcttcctct aagcggcggg cattgaaaaa gtgcgccgcg ccaaagcgga
    59941 cgttctgccg agccaagcgc tggtaagttc tcagcacccg aattctgaaa tagaaattga
    60001 gcactaccat ggcagcaaac aaggccatca ggctaaaaat cagagctatt tgcatggaaa
    60061 aacgggatat gaaacgtcct aaaaatgaaa agtgcgaaag ccgggcggct ttgcactttt
    60121 cggaaaaaaa ctaaacccca gttatagatc tttcagagtc gaaactcttt acatttttat
    60181 tttttaaaac ttccgatcat caagatgatc tttcagttgc tgttttttgc aatcacgatg
    60241 caaagataag cggcctttga taaatgtcaa gcttttcgct gatttttttt gaggcctcct
    60301 gctttttttc gtgtgggcaa aaatttctgt tttgatgttg gttaaaattg atgtatcaac
    60361 atttaacgat acaaagttaa gcctctttat tctgaaatgc aagttttttg cacaaaaaaa
    60421 tgtctcaaaa aatacggtgt gggcattttt gcaatccagc ggtagcaaac caaaagtaaa
    60481 cggcgtattg aatgccttga caatcaatac gccgcgttca catcaataag cagccgccat
    60541 ggcgtcgtct atgatctctt gtggaatggt agtaggacaa acaaaatctg ttttcggcac
    60601 ctgcgactct tccaccgccc gccagccacc gtaaacatca acgacattct ggaagccacg
    60661 cgctttgagg atcgaagcca taatcatgga gcgataccct ccgaggcact gtacatagta
    60721 cttttggcgt gcgtccaatt tgtacatgtt gttgttgatg tagtccaatg ggaaattttg
    60781 ggcgctgagg gcatgatgag acaagtactc ggagggcttg cgcacatcta agtaattgtc
    60841 aacaacgcca accttgaggc gaagcgcgag ttcatcggca gtaaccgact ctatggtgtc
    60901 cactgcgcgt cccgtggcag tccaggcttc aataccgcct ttcagatagc caagcgtatt
    60961 atcgtaaccc acgcgagcca ggcgggtgac cacctcttcc tcccgacctt cgggggtaag
    61021 gagtacgatg ggctgatgca aatccacaat caaagcgccg acccagggcg caatggcgcc
    61081 gtcaatccct ataaatatgg aaccggggat gaaaccctcc cggaaaacat cttcatggcg
    61141 ggtatccaat atgagcgcgc cctcgctctc cgtgagcaat tcgatctcgg ccgggctgag
    61201 tggtactgcg ccgcgtttga gcaccgcatc gaaactgccg tagccctttt tattgagcat
    61261 ggcatttttt gcaaaatatt gcggcggcgg cgccaaacct gctgtcacct cggcaatgaa
    61321 ctcatctttg gtcatatcgg cgcgcagggc atagttggtt tccttctgat ggcccaaggt
    61381 atcccaggtg tcgctgctca tgtttttgcc gcaggccgaa ccggcgccgt gagcaggata
    61441 cacaatgaca tcatctgcca aagtcatgat cttgttgcgg aggctgtcgt acagccaccc
    61501 ggccaggtct tcttttgtaa cggagccggc cttctgagcc aggtcgggtc ggcctacgtc
    61561 gccgatgaag agcgtgtcgn nnnnnnnnnn nnnngtaatc cttcccattt tcatccttca
    61621 gtagaaaagt ggtggattcg ggcgtgtggc cgggggtgtg caaaattttg aacgtaagac
    61681 gtcctacttt cagttcctcg ccgtcttggg cctcgtgctt gtcaaattcg gtggcggcgc
    61741 cgggaccata tacgatggca gcgccggtct ttttggccag gtctaagtgc cccgacacaa
    61801 aatcggcatg gaagtgcgtc tcgaagatat acttcagcgt agcgccttct ttttccaatt
    61861 tctccatata aggctgggtc tcgcgcaggg ggtcaatgat gacagnnnnn nnnnnnnnnn
    61921 nnnnnnatat aataagcgcc ttgagcaaga catcccgtgt agatttgttc aactttcatg
    61981 gtcaatactt tgattttaga ttggtgtggt gttcaaaacg gccatgtaaa aatggcatcg
    62041 caaagatgaa tgatttttaa cagctcagtt tgtgatggcg ttcacaaaag aaatttttaa
    62101 aaaaaacaga cttttttgtt taccccgcct cccctgcatt cattaacagg caaatcaaac
    62161 aaaacagact tgccatgaaa aagaaatcca tcttccacct cttccttttc ctgtggatac
    62221 tgactgcgtc tttcggctgc tttgaagaaa aggacttccc gccaggctca cagaaatttt
    62281 gcaagccctg ttttgaagac tctgactgct gttgtggatt gagttgtctg cccttccgaa
    62341 ccaatctcac atttgtacac aggtgcgcaa cgacaagcac acaaacctgt ccggaataat
    62401 gcttcatatt acaataccgt caacgatgaa aaagctactt ttttccgcct gtatttgcct
    62461 gtcggcagcc ggtatgctct gcgctcagaa aatgtcaata tccaatccga ctcctttcga
    62521 gttaagccgt gaactgacca ttgctattgc tccggcgagt attcgataca tggacttaaa
    62581 taccgatgca ctgctgccca aagagcgcta taacggcgaa gctgttgccg ccttttttga
    62641 ccgccgattg gaggccttgc tgctacaaca acagttgccg ctctgtgccg ggccagacag
    62701 cctgttcata ggagggcagc aacgcctgct tgcccaaggc tatgctttgg tacgggcttt
    62761 acaggacgaa aaaacggctt cggagttgaa aagcaatttt gcagatgcag ggtgccggtt
    62821 gataatggtt gccgacatca gagtgaaagt gggcgacgat gcggagtggg aaggcgccaa
    62881 tccattatat atatccctgg tagtacaccc gcaaacgcac acaacccgga taaaaacggc
    62941 cttgattgat acggcttccg caagcgtact ctggaaaaat gagcttgaaa ccaagggcat
    63001 tccagatgta gcaaagcctc gttgggcgga agtattcgag acgatttttt caaacatcaa
    63061 aacaaaatag ccatgaaaaa caacttagta caacttctga gatacagcgt tgtcctgttt
    63121 ggacttgcac tcgtcggctc ctgcattccg gcagcaaagc ctctgttttt ggattcaagc
    63181 ttccactcgg tcaagataga caccatcgcc gttttgccgg tagcagatat gcgtaccgac
    63241 aaaagtctgg agatcagcaa ggtggaccaa tggagggtaa gaccttttag aaatgcgctg
    63301 acaagagctc gatatgcatc cgtatatata gaaaatcggg cacttgttga caacatcgcc
    63361 gaagaagacc ttgaagaact tgacccaagt tggatcaaaa ggctaaaccc cggtcagcac
    63421 agatgggtgc tgttgcctat tatctacggc tctcacaaac aagtggatat tgggaaaaaa
    63481 gtacagttgg aaatagccgc agccttattt gatgtgcaaa ccggcaagcg catctggttt
    63541 cataaagacg cgcccaaatg cagcggcggc ttgctcatgc ccggcgtaat gcaagactgt
    63601 attcaaacga gctattgggc gctcgccatg aacctgccca agcagtaaac tacctgttcc
    63661 cgtttccaag ggctttcgcc ttgacccaaa cgcactcatt tcttcctgcc tgcgagatac
    63721 acttcacagg ccggccagct gctgctttgc ctgtccattt caccatcttt tcaaactgat
    63781 ttaaaaaaac atgccatgaa aaattcaatg tatttccttt ttaccacgct gttcttggtc
    63841 ttcacattat ccgcaaatgc cctcccgaat tgcaactcat ccaacaacgc ggatactccc
    63901 gaatggagcc ggggcatccc ggagttgtgg cagagcgata ccctgccctc cctgaaacct
    63961 gaaaatgggg aatggggctt tggtattcgg gcagaaggac tgcaagggct tatttggaac
    64021 aacaacttcg actcgctgag cttgcaactg agatacgccc ttcgcccatc tgccgttttc
    64081 agggttgatg cgggaatagc gctgtacaaa gaaaaaacct cgttgaaaga tgtattcagc
    64141 aatggtggac atcaatataa tgaagatgct gaaacccttt cttcttttag catttccccg
    64201 gcaatggaat ggcatttcaa tgggactcgc cggcttgacc cttatctcgg ccttgcttta
    64261 ccgctggttt ttgtgggtaa gcgccatatt actgaaatag acgaaaccat caacagcaac
    64321 ggcaccttct tcaaacgtga aatcctgaga gaactacccg gcggccttgg cttcggaatt
    64381 aatggcatcg ccggaatcaa ctacttcgtt tccaatagat tagctctggg gctggagtat
    64441 tccatcggcg cctcgttggt gaatgtcagc ggcaaaataa aaatgaaaac aaccaccaga
    64501 agcaaaccaa gccctacagg ctcggaaacc atcaccatca cggagagcgg cgacgacgaa
    64561 atcagccaaa gagacatctt tttcggcaat aaaggcgtag ccggcatcaa cctcattttt
    64621 tacctcggac gctgatatgc catacctcct gccatataaa taacatcaaa acatctcgcc
    64681 atgaaaaatc atccgctttc tctttcggcc agggtactct tcgcatttgc cttttccctg
    64741 ttttcgatgg tgctgtatgc ccaaagcacg atttcctgct ctcagtccac ctccgccaac
    64801 ctgacgaact ccagcccggt caacactttc ctcctctctg ccaacaacaa cgaccgggtg
    64861 cgcatacgag cctattcttt cgttgccggc ctcaacctga ggatagaggt aagaagcccc
    64921 gacaacagtg tttcgctggg ggtctttttt gccaacaatg gattggttga cattcaggaa
    64981 ctcacgcttg gggcggggaa tggccagtat cgaatcttgg tatctgatgc agaatccaat
    65041 accaccggaa cctataacat ctcggtacag gggctgaaca aaaccacctg cgcctcttta
    65101 gtttcgtgca gtgccagtag ttccggcagc tacttttaca gcgccgccat cgaaggtaaa
    65161 taccttgtag cctcggccgg cgatgtgctg cgcctgcgct acttttttgt tgaccccaac
    65221 cctgaatggc ggctcgaagt gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    65281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    65341 nnctgcgcct gcgctacttt tttgttgacc ccaaccctga atggcggctc gaagtgcgcc
    65401 gacccgacgg cacgctgcac gcgctgttgg cccaaaacgg cagcggattg atagatacca
    65461 ccatgctctt gcccgccgcc ggcaactggt ttttttacct atacgacgag gatggagacg
    65521 aggccaatac cacgcgtact tatcactatt cattccgatt attgaaaaca tcctgtgcaa
    65581 ctgcattaaa ttgcggggga agcgtgtcgg gttctatatt gtacagaccg gaaatagact
    65641 tacactattt ctacgcagaa ccatgtgaca cagcacgcct gttgttttcg gcagttgatc
    65701 cctcctttta cgaagccatc agcctgtatg ctcccaacgg cgccttgata ggtcagtatg
    65761 aggccgtcaa tagcagcgac ccggccatat tgcaagtacc tttgtcggcg tatggttatg
    65821 ggtactacct tgtggctgtg tatgaaaaag aaggcaatag ccttaccttt accaattaca
    65881 ccctatcgtt gtcctgcctg cgcagctcat tgaacgtcgc tcctgcgagc atgaatatca
    65941 atgcattagg agggaaccag acattgaatg tatcgggccc ctgccccagt tggtctattg
    66001 ccggttctgc caattgggcg acggccaatc cgaataccgg ttcgggcagc ggcccggttg
    66061 ttgtgacgtt cggccccaat cagacgacgg cctctcgttc tgctcaattg ttattagtgg
    66121 ggtgttgtga ttctacgttt gtcaacctgt ttcaatcagg cgccacactt tcggtaagcc
    66181 cctcctccat caatgcagga atagctggag gagcttattc ggtgaatgtg acaagtactt
    66241 gtattcaatg gacggtgagc agttctcaag actgggccac gccgtcggtc agtgccggca
    66301 gtggctccca aagcctgagc ataatgtgca gcgccaacac aacgggcttg ggccgaaatg
    66361 cagtaatcac cttcagcggc tgcggtatca acgtgaccat aaacgtagtg caaccggcag
    66421 ctaccctggc atgtactccc ggcaatatca acccaaccgg ggccggcggc gattataatg
    66481 taacactgag taccaattgc ccattgaatt ggatttcgtc cgttgcgcca tctgccggca
    66541 tttggataga agtgctcccc gtattcggca atggcagcgg caatgtagtg atacgggtat
    66601 cccccaatga cgcgccgata gcccgttccg gaacagtaga attttcagct tgcgacctga
    66661 cccggagtat ctccgtcgtt caatcaggag ctactttgaa cgctactccg ttcaacttca
    66721 ccatatcagg cagcgcggcc gggtcagtcg tttgcaatct cagcggcacc tnnnnnnnnn
    66781 nnnnnnnnnn nncatcacgg gcgctccaga ctggcttacg gtaagtttgt cgggcaacgc
    66841 actgccggca acagccacgt tgctctatac tgccaacaac agcggggtga cccgcacggc
    66901 tactttgacg ttaagcggct gcggaataac ccggagcatt acagtaacgc aacccggtgt
    66961 cctgatcatc agcggcagca cgagttattg tcagggcagc agcacccagc tttgcgctcc
    67021 gacggcagat gcctacctgt ggtccaacgg cgccaccacc agatgtataa ccgtttcgac
    67081 gccgggcctt tacaatgtag tcattacaca aggcggcaca caacaaagcg ggtcggtaac
    67141 aatactggaa ctgccggctc ctttcccgcc ttttattgtc caatttgatg ccaatgtgct
    67201 gctcgcagta ggcgatgctt cttcgtggag atggtatctg aacgggcaac tcattgcggg
    67261 cgccaacgga aactcgctac cattcacggc gggaggcagc tataccgtga ctgcggtggg
    67321 cgccaacggc tgtgaaagcc agccgtctgt tccgttcctg ataacatctg tgcatgagcc
    67381 ggcgaaagca ggcagactca aagtgacacc caacccctgc tcggaatggt ttgaggcgga
    67441 gattcccgac cgcgtcattt ctgcccgctg tttggtgttc gacgcatacg gccggctttg
    67501 tttcgataaa aaagaggtga cgggacaggt agtcagagtg ccaagaaacg ggctgccggc
    67561 gggttggtac ttgattcgga ttatagacaa ccgcgggact gtttctgcaa caggcaaggt
    67621 tctctttgtg gattactgaa accgccggaa caaccacaca taatcagtaa cttatgtcaa
    67681 atacaattct cgcagaataa tttactttcc ttaccttcgt gcaaattaat ctgatttgtc
    67741 tgaatcttat ttcaggcaaa cgcatcagag ccgacgaggt atggaaaaac aagacttgag
    67801 ctttaccgac gagcagattc tggaatttgt aaaaaacaga aaaatgcatc aacgggtctt
    67861 ccaatgggcc gactctaaag tgttgcccgg catccagacg atgatgaaac acagaggcaa
    67921 cccgtcggaa aatgacatac gcactttttt cctgacggca ctgaccgact tcttccacat
    67981 actcgaagcg ccggagtatc aactgcgcga aaacagcaat ctcaacgggc tgcttttcac
    68041 catttgcgag cgcatgtgga tacgagagtg caaaatcaga gggcgttttt ccggcggaga
    68101 tatgcccgac cctcctgcat catcgtcctc cgaccacgat ctgattcatc gcgaaaaact
    68161 ggcgatggtc agcgcggccg tttcccgctt ggagcagcct taccggggag tattggtact
    68221 gcacgaatac ggcgaactca gccatgccga aattgcgctg gcagccgggt acgcttcgcc
    68281 ggacgtgtct aaggagatca agtcgagagc cataaaaaag ctgcgcgaga tttataaaaa
    68341 cataagctga aagggatgga cagtcagaat aaagatttcg acctgctgtg cgatgattac
    68401 ctgcacggag ccatgtcgga ggccgacaag tctgagtttg aagccctgct gcaaagccac
    68461 cccgaacatg cggctcagtg gcaggagttt tgcatcctca cgcagggcgt acgcgagtat
    68521 gggcgccgac aaatgcttga aacctggtac aacgaggcgc atggcaaagc cgatgatgaa
    68581 aaaccggcag ccgggaaaca gccaacgctg atggacatct ggaaagcgca acaaaaagcc
    68641 gctgccggag agtcgggcgc ctcttccggc aagcgcgcgc tcccgatatt ccgatacatt
    68701 tcggcagccg caataatggt catcgtcttt tttgtaggtt ggagattggg aaacaacacc
    68761 gacccagaga caggccgggg agatgatgtc atcttgctcc gtcaaatgga gctgccggtg
    68821 gtgtccattc agacaagaca ggagtaccgt cctgatacca atgctgaaat ctggctggtg
    68881 agggtggaga aaaacctccg ccccgaatct gtcgccatcc tttccggccg gcaactaacg
    68941 ctggtactcc cgacttcgga agctcccgac gacatccgct tgcgcaggct ggacgggcgc
    69001 gtctatatcg gtatgggagg cgggtggtac ccactccgca cggatgagca acgcgagcaa
    69061 attttgctac ctgttgagga tgtctctatt ctctcnnnnn nnnnnnnnnn nnnnnnnnnn
    69121 nnnnnnnnnn nnntcggtct tttgatgttc ttggcaatat ttatgagctt ccttgtcccc
    69181 gccggcgcgc aggatgattc gggcgtttta cccgaagacg aacgcgaaat gaaatcagtg
    69241 cggcagttcc gcgccactga acgttctgga cagtatcctt cttttttttt gttcgacaaa
    69301 ttagccatct ctcctgagaa gtttttttgc aacggctttc ctctcgatca ggggcgctgc
    69361 gcctcttgtg caggctgcgc cattgcacag gcatactcta tacactatgc cttttggact
    69421 aaacagaaaa acaggaagcc gagctggctc aaattttcag gctcctttgt atataaccag
    69481 atcaaacaac ccggagactg tcgccagggt gcccgcatca gcagcggact gaaactattg
    69541 accgccaaag gtatttgccc cgccgacaca ttcccgcatt ccccctacac ttgcggcgat
    69601 ctgccccaaa aacaacactg gaaagttgct gccggctaca aaattgccgg cttcgagaag
    69661 gtgacaacgc ccgatatgaa ggccaaaccc gactcggtca ttattaatgc actgaaaaat
    69721 gccatagttt actccaaccc cgtcatagcc tgtatccaga tacccaaaag cctggcggaa
    69781 ttgccggagg gcgagtgcag gtggcaggca cagggcaagg aaagtactta ttggcacgcc
    69841 gttgtcatta ccggctacga cgaacatcat tttcagatac tcaacagcaa aggttgtcgc
    69901 tggggctgca acggactggc gctggtgcct catgctcagt tggcctccat gctggaatgg
    69961 tcttttgtga tgacttattt accacgttaa atcacaccgc tatgattacc caaatatgcc
    70021 gcccactgat gttcgcgctt tgcctcgcga cgattcaggc cgccgcacag cccaattgtt
    70081 atgcgctgtt gctgaaaagc gatacgctca tgggacgctc caagtggaaa gaagccgccc
    70141 ctcttttgga ggaggcgtat cgggtttgca tggaggaaca tggctttgaa agcgatgaaa
    70201 gtgcatggat tcacaataat ttgtatatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    70261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    70321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    70381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttc
    70441 aatcaacatt agacccccgg catccttact atggaactct ttttaattcc ttgggcattg
    70501 cttacggctc cattggatat tttgacaaat caattgaagc gacaacagcc gcattggaac
    70561 tggaaggaaa gatcaataat aaactgtctt ccatttatgc caaccgactc cacaatatca
    70621 gcacaattta tttgctgaag ggtgaacagg aaagggcaat ccctctgctc catcagtcaa
    70681 ttgctctgtt ccnnnnnnnn nnnnnnacaa ggagatacgc tctctttgca atacctcact
    70741 accatgcaaa cgcttggttt attctatcac ataagcgggc agtatgaagc agcgctgaag
    70801 cattgcgaca attatctttc ttttttagaa aatcgaaaat taacccaaca tcctctttat
    70861 caatactttt taaacacaaa attgacgatg ctggtactta caggccaatt tgaagaggtg
    70921 gagaagttat acggacgcca tctgaatgaa ctaacgtctt ctgatggtga taacttgacc
    70981 aaggctttgc tgatggagaa attggccagc gcttatttat tctccaaccg accggaaaaa
    71041 gcgattcccc tcatagaaaa ggccattgaa attgtccaaa aaaacactgg tgaaagttcc
    71101 tattacagtc tggggctgaa gtcagtatat gctcaattac ttttcctcag tgggcgtaca
    71161 gaggaggcca ttgccgtagg agaacccaac ctgttaaatg ttatctctgc gctcggagat
    71221 ctcgctccgc tatctgccgg gcaggaagcg gttttggcga aatgctatgc ttcaaccgct
    71281 caattagaca aagccctgac ttcagcgcga aacagcctcc gccgtgccga tgccattttc
    71341 ggagagcagc atccgcagta cctggcttat ctcgctttat ttgtcaatac actctacttg
    71401 tcgggtgaaa ctgccgaagc cgacgcacat tttgttcgcc tcaccgctct gatacaatcc
    71461 aacctgctca gattttacag tttttttgca cccaaagaac agctcaatta ccttgcactc
    71521 tcccagacta atttagaatt tattgaacag tacgtctatt tgaattcgaa ccgcaatccg
    71581 gagcttgcta caaccttgtt caacagcaac tcctcgctca aaggcgccct cctcgacgac
    71641 tccaaagccc gccgggcctg gctgctccaa aactcggaca gcgccaccgt cgtattgcaa
    71701 aaggaatgga ccaacatcgg gcaagccttg gccaaacaat acgccttgcc tatcgaccaa
    71761 cgcaccatca aagatgccca aatattagac agcctccaaa atcgcttcca aaaattggaa
    71821 caacagcttt ccgatgcctc cgcagccttc agaaaagcat ttgccggtaa annnnnnnnn
    71881 nnnnnnnnnn actggaatac cataaaaaac aaactccgcc ccaacgaagt cctcatagac
    71941 tttttcagtt tccggctgta tgatgcgagc tcagacacgc aggccgattc tgtgctgtat
    72001 tgcgccttag tgggaagacg cgattgggcc gcgccccggc tggtgccgct gtttgaagaa
    72061 aagcaattgt tgcaacgcct cgccttggcc gacgaaccgc tgggttcggc tcaaaacgtc
    72121 atttccagca tatatgatcg ccatttgtac caaatactct ggcagccttt ggaaccgtat
    72181 ttgcaaggcg cctccaccct atggctgtgc ccggaaggcg ccctgcacaa gatcgctttt
    72241 gnnnnnnnnn nnnnnnnnnn nnnccaatgg gcgttttgca gggcaaaagt acaggttgca
    72301 aatgctgggc agcccgcgag acatcctcca ttacgagctt ttggagctgg accgcatcaa
    72361 cgacgccctg gtatatggcg ccatccaata cgatgtgagc gaggaattgt ctgattgtat
    72421 ggagtcaaaa attccaaaaa acgatgcgcg ccaactcgcc tccgctctcc gcaacagcgg
    72481 ttgcggcact cgactgcctt atcttgccgg aacagatgca gagagcgatt tcatcaactc
    72541 attattgaaa aacaatcgca ttgagacggt ttcccaaaag ggctgcgaag cctccgaaga
    72601 ctactttaag tacgcttgcc gaaacaaaaa aggcgggttt tctttgattc acctccctac
    72661 acatggcctg ttctgtcgtc cgccggtact cgccgttccc gaactcaata aaaactcaga
    72721 cattgcgccc aatcctttgt tgtactcaag tctggccctg gcagcctaca accgaaaagc
    72781 aagcaaccgg ccattgcctc cgcatcagga agacggcgaa ttgacggctt atgaggtttc
    72841 ccaacttttt ttaccccaaa cccaactcgt cgtactcagc gcctgtgtga gcggattggg
    72901 cgagacccaa agcaccgagg gcgttttcgg acttcaacgc gcttttcgca tggccggcgc
    72961 gcgccatgtt ttagtgagcc tctggcaagt ggacgaccag gccaccaacc aactgatgca
    73021 attgttttat cacttctggc ttgtcaaaaa aatggatgca gcgcagnnnn nnnnnnnnnn
    73081 nnngggcagc acaagagaaa atgcgcaagt caaaagagtt tcagcatcca tatttctggg
    73141 caggatttgt tttcatctga aacgggaagt tccctacctt caccccttca ttcacctccc
    73201 gcatcatgag caaattcctc gtcctcctct ctgccttcgc actgtatagc tgcgccggcg
    73261 ccgcccagat ggcacagccc gccggcgcca cgcgcaccct cattgccttc ggttcctgcg
    73321 ctcaggaagc caaaccgcag cccatcctcc aacaggtagc cgatctgcgc cccgatttct
    73381 tcgtctatct cggcgataat atatatggcg acacctacga catgaacgtg ctccgcgcca
    73441 agtatgccca attggctgcc aagcccgaat accaagccct ccgccgcgcc accaccgtac
    73501 tcgccacctg ggacgaccac gacttcggct ggaacnnnnn nnnnnnnnnn nnnnnnnnnn
    73561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctgccgattc
    73621 cgaccggaga aaacgggagg gcatctacac ctcctaccta tatcattatg gcggcaaaaa
    73681 actccagatc atcctcctcg atacccgcac ttttcgcgac aacctccgca actaccgcgg
    73741 cgaactgcac aaccagagcc gctacttcta cccgctcgac tacttccccc accaaacctc
    73801 cgattccacc ctcctcggcg aacaacaatg gcgctggctc gaaaccgaac tgcgccgccc
    73861 cgccgatctg cgcatcatcg gctccagcac gcagtttggc atcgaattca atggctacga
    73921 agcctgggcc aatttcccgc acgaacagca gcgcatgatg gacctcatcc aaaaaacccg
    73981 cgccgagggt gtcctcttca tcagcggcga cgtacactac gccgaaattt ccaaaataca
    74041 gccgcccggc gcctacccca tctacgacgt cacctccagc ggcatcacct ccacctggca
    74101 tttccccacc cccaacgccc accgcatcga ggggccggtc atggaaaacc acttcggcct
    74161 gctcagcatt gactggaaac ctgccgaccc gcttatccgc atggaggtga tagacgcgag
    74221 cggcaattcc cgcatagaat atacagtgcc gctcagtcaa ttgaggttct gatctgatgc
    74281 tgatttcggg gcgccccggc ctctctccaa cctcccccag cccctccaaa ggagggtagc
    74341 cacaggcggg ccgtcgggtc attcgctaca tgcctgcagt cccggctgtt gcaagccctc
    74401 tgccggcatc cgctactgcc cctggcgcac aagttgttac cctccccgac tccaagatat
    74461 taccaaagcc ggcacatcgg ccatactttg ccgaaacatc ttcggcagga ctaccatttt
    74521 ggcgccgttc agctcctgca ccttcaactt cccggcatgg cccagcaatg tcacctgttg
    74581 ggccgcgccc acaaaatcgg ctggcagttc caggcgctcc ggcatggcct cgtcttcggc
    74641 ccgcaggtag atggcgtagc gcgtgctgcc gtctttcgat tgcgtgaagc aaaatttgtc
    74701 cgattgatag ggcgccagcg ggcgggtatt gtaaatggca gcggagttca cgtccatcca
    74761 gcggccaatg gcatccaaac gctcatacac tgcgggcggc agttcgccgg ttttgtcggg
    74821 gcctacgccc aagagaaaat tgcccccttt ggccacaatc ttagccaagg tatgtatcgc
    74881 ccaggtactc gatttgtagc gctcgccggg gccggtgtgg taccaactgt cgcccatcgt
    74941 gatgcaactc tcccaggggt agtcgggtac atcggcggga atttgctgtt cgggggtgcg
    75001 gtagttctcg aagtcgccat gcaccgtgcg gtccaccatc agcaggccgg gctgtttgcg
    75061 gcgcgccatc gcagctatgg cgggcatgtt cacgtcctgc gtccattggt ttttgcccaa
    75121 ccagggccgg gtttccgccg tcagcgagcc ggccgggcgc acccatccgc cgtcgagcca
    75181 gagcaagtcg agcttgccgt agtcgctcat cagttcttca atctggttgt aggtgaattc
    75241 ctgaaaccgt ttccaacgct ccgggtattt ggtcgggtcg taattcacat tcctgtcaaa
    75301 tacagggaaa tacggccacc agtagtcgtc gctgtgccag tcgggcttgc tgaagtacgc
    75361 cccgataccc atgttttgcc ggcggaatgc gtcaaacacc tcgcgcacca cattgctgcg
    75421 cgggtcggag gcaaaaatgc tgcccgcacc ggtgatttta taatcggtgt accggctgtc
    75481 gtacatacaa aaaccatcgt ggtgtttggt cgtaaacacg aggtatttca tgcccgcgcg
    75541 ccggcaggct ttcgcccagc gctgcgggtt gaactgctgc gggttgaaca ctttccctat
    75601 ttgctcatat tctctcgtgt atgcggcgta gtcgtctgca aaaggcccgc gtcgctcgca
    75661 ccagggttca tcttcggggc agaggctcca actttccacc acaccccatt ggctgtacgg
    75721 cccccagtgc atgagcacgc cgaatttcca gtcttgccag cggtgcaact gctcgctcac
    75781 agccgggtcg gagggccaga tatagggcgc ttcgggttcg tgtgtttcgg agggggcatc
    75841 ggtcagtccg ccctcgccgc gatacagttt cagcggttgc ttgagttgca cttccaacac
    75901 ggtgaggtac ttgtcgtgca aatgcgcggg caccttggca tacaccaaac ccggcacgct
    75961 gctccaggat attttgccta ctattttcca gtccaaaggc gtctcgctgc ccactaccga
    76021 gatgcgttca atgtcggaag ccagcccttt gaggatgatg tcctgacccg gattgcccgt
    76081 caggaacagg tacaggcggg tgctgtcttt ggagagggtg ctggggccgt aaaaatgacc
    76141 ttgcggcaag cctgccaccg taccgaagac tgcgcgcccg tgtttgcgat tccaatcacc
    76201 taaggcttcc aacacctgca cctgttgtgg cgggatggtt ccgtcctcca tcggcccgat
    76261 gtcgagcagg aggttgccgc cgttggctat ggcatctgca aaaatggtga tgatttgata
    76321 aggcgatttc caggcggtgt cgcgatgctg aaaaccccag ttgttgttga tggtcatgca
    76381 cagttcccac catttgtgag gaggtctgga aaccggaaaa ttctgctccg gcgtggcata
    76441 atctccgtag ccttgcagtc gcccgttgac gatggcgtca ggattacggg ccaggatgga
    76501 gcggcgaatt ttggaggcct cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76561 nnngaaccac cagagatcgg gttgaaaacg ctcgccgatt tcgtcaattt gcccctgaaa
    76621 aaagcggcgg aatcggttcc agcgggccgg gtcgtttttg atttcgtagc ggttgctgtc
    76681 tttgaggaag cccggataat cggggtgcgt ccagtcaatg agcgagtagt acgcgccggt
    76741 tttgatattg cgctttcgga gttcgtcaaa caggggcgct atcatatcgc ggcgagccgg
    76801 cgtcgcgtgc aggctactga ggcggttcat ccgggtatcg tacatggcta cgccatcgtg
    76861 gtgtttggtg gtgatgacgg catagcgggc gcccgattgc tgaatgagat cggcccaggc
    76921 ggcggggtcg tatttactga gggtgaagcc attgagttgc tgcatgtact cggcatggct
    76981 gatttttttg ttgtaaaaac tccaggactc atccacgcct ttgacagaat aaatgccggc
    77041 atggatgaaa ataccgagtt tggcatcggc gaaccattgc attttgcggt cgtcggaggg
    77101 ctgttgggcg ctcgcaggca gggcggcggc gaggaggagg gcgaagagga tgtgtcgcat
    77161 gttggtagtg tttacagagg gccaagatag gaaaaaacct tacctcggcg gccccagcgg
    77221 cttcaaatcc ttgaggcgtt ttctcagttg gcgcatgaat tttttgccgg ggtccacctt
    77281 attggtaaaa taattcggat cggtttcttt ccacaagtcg gtggttttga aacgcaggta
    77341 ctcgtggtga ccgatgaggt actcgatggg atatcgccca aacaactgcc tgatgagcag
    77401 cacattggct tcaagctgag ccttcgtgag tttgtgtttg cggccgtcgc ccacgttttc
    77461 gatgccgatg gacatgtagt tcagcccgat gcaatgcctg ccgaacagcg agtcgggcat
    77521 gaggcgatac accgtgccgt cgcggtcaac gaggtaaggc accgagacat tgagcgtact
    77581 ggcattgacg agttcggggc gagcgcgcag ttccaccgga tcgaacgcgc ggaaggagcc
    77641 ttccagcgta gggatggccg tccaatgcac cactaccatg cgcggcgcga tgaccggttc
    77701 gggctgaacg atgccgtgcc gtttttccaa atattccaac gacagtcggg cgcgggtgct
    77761 gtcccagaca atggggcggt ccacgatgcg aagttcctgg ccgaggagcg gccctgcgac
    77821 gatcaaaaac agggtaaaaa gaaaaagtgc gcgcatatct cgaatttttg aaggatatta
    77881 ccggcaaatt tggcaaaaac atccatgagt ttcctcaaac gcctgttcaa aagtaccgat
    77941 tcgcagcccg ccgtacccga agtgcgtttc ggccgctatt ccgattctta caaagaaacc
    78001 gaaaactaca aggcttggga ccagtcggta gaagcctttg aagaaaagca gtacttagat
    78061 tcactgcggg ctttcctgcg ctatctccgc gacgaacacc aggacaatgt gcattggcaa
    78121 gatcatggcg accgcattga gtttgaaatc tgccaaggat cgaaactcat ttcgggactg
    78181 gcagatgcgt tgcactttaa agccgaatcc aaggtggcaa aggccaaaga tttggacgtg
    78241 gccttcatgc gtcgtctggt ggagcagaat ttcgacaacc tttactcccg atacgcgctg
    78301 gaccccgaca acaacatcac cgtagtgttc gacactttta cgctcgacgg gtcgccgtac
    78361 aaaatttacc acgcactgaa ggaaatggcc acccatgccg acaaacagga cgacctcctg
    78421 ctggatgagt tcgacagctt ggagccgatt tcctcccctg cgctcatgcc tgtgccggac
    78481 gcggaaaaac agaccaagta cgactacatt tgccgggaaa ttgacgcggc cctgcgcgtg
    78541 atggaagatg gccgtttaga caaaaaccag tatcccggcg gcgtgggcta cctgctgttg
    78601 tgtttggttt ataaattgga ctacctcaca cagcctcaag gctttatgat ggaaacattg
    78661 gagcgggcgc accggctgta tttcaccaac gacggcaaaa ctaccgcaca gaagaacctg
    78721 gctttggcca aagaattgca aaccctccgg cagcgctctc agaccctgtt ttacaaagaa
    78781 atgtaccgcg catcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt caatcagcgc
    78841 cacatcgcgg gcttcatcga gtcggaattg cccaatatgg actggtactg tgagaacggg
    78901 cataccgaag tggctttgtc ggtgccgggc tacattgcgg ggtattgttt gttcaattat
    78961 gctccgccca ggccagtgaa agacctgctt catttgtact ttcagatcac ggaggcgggc
    79021 tttttcaaac atctgggttt tccggctgcc atgtttgagc ctgaaacggg cgtatttcac
    79081 aaaaaagcca tccgccaggc cattgagggc attgtggaaa aaaaccggga gcgctttcat
    79141 ttgcaacccg acctgtcgct gctgcaatgg aacgacctgc ccactttcgc gcgctcttta
    79201 ctggctatgc tggcacgcat ggaggtggaa acgcggtttt gagcggtggg cggtgggctg
    79261 ttggcggcgt ctcacgagaa gcaaccgcct accggacacc tcgcgcttac cgcgccacgg
    79321 cgcagtaccg cccactgcct atcttaaatc cgattctgat tgatcaaatc cagcagctcg
    79381 tcgcggtagt ttttgccgat gggcaagtgc ttggcctggc ctttttccat cacctctacc
    79441 atattgccta cgatggcgtg taccttgttc aggttgacga tgaaggagcg gtgaatgcgt
    79501 ttaaaaatag ctgtcggcag cttgtcttcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    79561 nnnnnggctg gcatcgagct tgatgatgac gtaatctttc agcccttcga tatagaccac
    79621 atcgtcgaaa ttgatcttta ccaatttttt gtcggccttt acaaaaatga actcatgcgc
    79681 gggatcgtgc gcggcctcgg tgagcgccgt tgcgggcgat tcgcggcgtt tgggggccaa
    79741 ttgttcggag gctttgttga cggccttcat gaagcgttcc agcgagatgg gtttgagcag
    79801 gtaatccagc gcattcagct cgaatccctc caccgcgaag ttggggtacg ccgttgtaaa
    79861 caccaccaat ggcgggtcgg agagggtttt caaaaaatca atccccgtga gttgtggcat
    79921 ctgaatatcg aggaacatca ggtcaatctc gtggtttttg agcgcttcgt tggcttcaaa
    79981 agcgttgttg cacttttgca cgagatgcag gcccggcact ctgccgatgt aggtttcgag
    80041 cacgtcctgc gccagcggtt cgtcgtcaac aatgattacg tttatcatga cttttcgatt
    80101 ttatgaatgt aaaataagta ttttcagttc aggtccattt ccaaagaaac ggcataagtg
    80161 ttcggattgt ctttgacgct gagttcgtac ttgcccggat agagcagttg cagcctgcgc
    80221 cgcacgttca ccaacccgat gccgccgctg cgtttgcggt cttgtacggg cttggtgtct
    80281 ggtttggtgt tttcgataaa aaaggaaata tgccgccctt ccacctgcaa ataaatgtgt
    80341 acaaagccct gtgccagcgc gttggtaagg ccgtgtttga agctgttttc caaaaacgga
    80401 atgaacatca gcggcgcaat ttgctgatcg gccaccatgc ccgacacctc gaagcgaatg
    80461 tcggcatttt tgctttgccg cagcttttcc agatcgagat aattgtgaat gtagctgatt
    80521 tccttgctca gcggcacctg cttttcattg caatcgtaga gcatgtagcg catcatttcg
    80581 gagagcttga tgacgatttc gggcgcgtcg tccgattttt tgagcgtgag cgcgtagagg
    80641 ttgttcagcg tattgaacaa aaaatgcggg ttgatctggc ttttgagaaa acgaagctca
    80701 gactgcatcg tctggttttc caattcctgc cgttcgcgga gttgcttggc ccagtcggct
    80761 acgattttta caatagaaga agccccggcc acgaagaaat gcaccagaaa ataccagttg
    80821 agattttcga tcagttcgcc gcgggtgagc gggtgagcgg caaacttgac ataaaaaacg
    80881 atggccttga tgggcgtaat gatgacggca gccaggatga gcagggccgc gtaggtgagg
    80941 aatttgtttt tggttaaata attggggatg agatagttga aattgaaata aaccaaaata
    81001 ccgtagaaaa aaacattgat ggactcgttg gtaaaagcaa accaaaagcc attgtgcgcc
    81061 ccctccagca aagtgagtaa aatggccaca aacagccaaa acaggatgtg ccgactcgaa
    81121 gtacggtaca ataccctgtt gaacgatgct cgccaatgtg ccggcagtgg attttgcatg
    81181 gcgcaaatgt gttgattcaa cggttgagtg tcgaaaaatt ttagaccaaa agcggattga
    81241 accccgataa tacacaaaac ttgccgcaag tgccttagtt ttgcacatcc ggcagagcgg
    81301 ccatccctgt caacccggta gttctgtttt ttacaaacaa gataaagcgc tatggatcaa
    81361 caggaaagcc ctgaattcga cagtaaagtt acccaaaacc tgatgaatgc atacacacaa
    81421 attttgggac aactcaatga tgacgcccaa cgcgaaggac tgctcaaaac gccggagcgc
    81481 gccgccaagg ccatgcaata cctcaccaaa ggctatgagg aagacggcga ggccattttg
    81541 cgctcggcta tgtttaagga atcttacagc gaaatggtca tcgtgaagga catagaattg
    81601 tactcgctgt gcgaacacca cctgctgccg tttttcggca aggcgcacat tgcgtacatc
    81661 cccgacggct atattgtggg actgagcaaa atccctcgca ttgtggaggt gtatgcccgc
    81721 cgcctgcaag tacaggaacg cctcaccgac gagattttac actgcatcca aaacaccctg
    81781 aaacccatgg gcgtggccat cgtcatcgag gcgcagcaca tgtgcatgat gatgcgcggc
    81841 gtgcaaaagc aaagctcgct caccaccacc tcggccttca ccggcgcatt ccggcgcgta
    81901 gaaacccgca ccgaatttct caacctgatc ggcaacagat tgcattagtt tacacattac
    81961 catgatcttc tccatacaac cctacgaacg cgaaatcttc cgcatcatca gccagacggc
    82021 ggcccgcctc ggctatccgg cttatgtggt gggcggctat gtgcgcgacc ggcttctggc
    82081 ccgcccctcc aaagacatgg acatcgtgtg cgtgggcagc ggtatccagc tggccgaaga
    82141 agtggccggg caattgcgcc ccatcccaag agtagctgtg tatcaacgct ttggaacagc
    82201 catgctgcgg cacggcgacc tggaaattga gtttgtgggc gcccgcaagg aatcctaccg
    82261 gcacgattcg cgcaagccca gcgtggaaga aggcagcctg gaagacgatc agaatcgccg
    82321 cgattttact atcaacgccc tcgccgtgag cctgaacgaa gtggatttcg gtacgatcat
    82381 tgaccccttc aacgggctgc aacacctcga actcaaaagc atacaaacgc ccctggagcc
    82441 gggccgcacc ttctccgacg atccgctccg catgatgcgc gccatccgtt ttgccacaca
    82501 gttggatttc aacattgagg agcaaacgct gcaagccatc ggccgctaca aagaccgcat
    82561 caaaattgtg agcatggagc gcatcgccat cgaactcaat aaaatcattg attctaaagt
    82621 gccctcggtg ggtttcaaac tgctgttcga caccgggctg ctgcacctca tcttcccgga
    82681 aatgacggcc ctgcacggcg tggaaaaacg gcagggcaag tcgcacaaag acaattttta
    82741 ccacaccctc gaagtgctcg acaatctggc ccgcagcacc gactcgctct ggctgcgctg
    82801 ggctgccgtg ctgcacgaca tcgccaagcc tcccaccaag cgcttcgaac ccgaacacgg
    82861 ctggaccttc cacgggcacg aggtgctggg ggccaacatg gtgccgcgca ttttcggccg
    82921 cctcaaactg cctttggacc aaaaaatgaa gtatgtgcag aagctcgtcc tgctgcacct
    82981 gcgccccatc agcctcacca aagaaaacat caccgactcg gccatccgcc gcctgttggt
    83041 ggacgccggc gacgatctgg aagacctcat gctgctgtgc cgcgccgaca tcacttctaa
    83101 aaatccggcc aaggtgcagc gctacttgga aaactacgaa ttggtgcagc agcgcctggc
    83161 cgaggtggaa gccaaagacc acctgcgcaa ctggcagccg cccatcaccg gcgagatcat
    83221 catggaaacc ttcgggctgg agccttcgcg cgaggtgggc gccatcaaaa acgcggtgcg
    83281 cgaggccatc ctcgacggca ttgtcgccaa tgactacgag gcggcttttg aatacatgct
    83341 ggaacgcgga aaggagatgg ggctggagcg ggtgtgacca atggaatttc acgactccct
    83401 cgcgccaagt tttatgagct gcccgcgaca tcctctgcat cccgtgaatc ctgtctaaaa
    83461 aataaagagg ccggatttcg tacaggcctt aatttgacaa atctcgaaga tttgtcaaat
    83521 ttctcctcca cctcggaggg tagtgaaggg ggtgactggg cgagtttcag cgggggtagt
    83581 caccccttca ctaaactgga gagccgtacc attgcttttg ccgaaatcat tcgtactttt
    83641 gcaacgcatc gcgccaggga cggacgcgga tgcagccctc ctgccatagc gctgcgggct
    83701 gcatggagca gacggcccga cccgccgccc tgatggtgca gaggctatcg agggaggtgg
    83761 cctgtggcgg ggggcgccca aaaagaaaaa aagactcaaa atatgcgcat ttcatcttac
    83821 ttcgtgttga cgatcctgct gaccatcgcc gctgttgcga atgtgtcggc tcagaagaaa
    83881 acttttacgc cggaagccct gtggaaactg gggcgcgtgt cgctgcaaga tgtttcgccc
    83941 gacggcgcga cggcgctcta taccgtgacc tactacgata tggcggccaa caaaggcaat
    84001 accgatatct atgccgtagc caccgacggc agccgcccgg cgcgcaaact cacggctttt
    84061 cccggcagcg aaaacaatgc ccggttccgc ccagacggcc aacgcatcgg ttttctggcg
    84121 ggcggcatcc tgtgggaaat gaatcccgac ggctccgatc agatgcaggt gggcagcagc
    84181 cccatcggcg gcttttgtta cagcccgaaa ggcgactaca ttgccttcat tcaggatgtg
    84241 aagtaccgga aatcggtgca ggacatctac cccgatctcc ctcatgccaa gggccgcatc
    84301 atagaccaac tgatgtaccg gcactggaac tactgggatg actacagcga tcccaatgtg
    84361 ttcattcagg cgtacggcaa cggccaactg aagggcgagg cgatgaacat agtgggccag
    84421 gccatagagg cgccggtgga gccttttgac ggcattgagg aggtggtgtg gcatccgggc
    84481 gggcgcaaat tggcctacag cgccaaacgc caaacaggta ccgagtatgc caaaggcacg
    84541 aatacggatg tgtttttgta cgacttagac gccaaaacga cgaccaacct gacggagggc
    84601 atgcctggct acgacaagca tcacgcgttt tcacccgatg gccgctactt agcctggctg
    84661 agcatggcga cgcccggcta cgaggccgac aaaaaccgct tgtttgtgct tgattttcag
    84721 acgggtaaaa aagaagacct gacggaaaaa tgggacgaga gcatagacgc catgcagtgg
    84781 gcgcccgacg ggcagagcct ctatttcggt gcgggtgtga acgctacgaa gcagttgttt
    84841 cagatagaca tggcaaaaaa acgcgtgcgc caggtgacca aaggccagtg ggatgtgggc
    84901 gcttttgtgg caggaaagcg ccacatcata gccgccagaa acgacatgaa cacgccggcg
    84961 gaactattcc gcatagaccc ggctaccggc gatatgcagc cgctgacctt caccaaccgc
    85021 gatatgctgg cgcagtttga cgacatcagc gtgcgcaagc gcatggtgaa aactaccgac
    85081 aaaaaagaca tgcacgtttg ggtggcgtat ccgccgaatt ttgatcccaa taaaaagtac
    85141 ccggcgctgc tcttgtgtca gggcgggccg cagagcttgg tgagccaatt tttctcttac
    85201 cgctggaacg tgaaactgat ggcctcgcag ggctacatcg tggtggcgcc ctgccggcgg
    85261 ggtatgccgg gatttggtat ggagtggaat cgccagatca gcggcgactg gggcgggcag
    85321 gccatgcagg acctgctgtc ggccatagac gacgtgaaaa aagaaccgta tgtggatgcg
    85381 gaaagattgg gcgcagtagg cgccagctac ggcggcttct ctgtgtattg gctggccggc
    85441 aatcaccaga aacgcttcaa aaccttcatt tcgcactgcg gcaccttcaa ttttgagagt
    85501 tggtacggcg ctacagagga ggtgtttttt gccaatttcg atctgggcgg ggcgtactgg
    85561 caggaaccga agccggtgtc ttatgacaaa ttttcaccgc atttgtttgt caaaaactgg
    85621 gacacgccca tcctcgttat tcacgacgaa aaggacttcc gggtgccgat cggcgaaggt
    85681 ttgcaggcat ttcaggcggc gcagttgagg ggcattccga gccggttttt gtacctgccg
    85741 gaggagggtc actgggtgtt gacgccacaa acgagcctgc tgtggcagcg ggtgtatttt
    85801 gaatggttgg agcagtggtt gaagggttag atcacccctt ccttccaaaa tccgccggaa
    85861 tttcgcccca acggtcggtt tcccatttca gcagcggatt gctccaactg tgctgatgga
    85921 gccagctttc ggcgcggcgg atgacctcgt atagttcttc ggtttgggcg ttgtgatcga
    85981 gttgggtttt gcaatgcttt ttgcgtaccc agcccatcgc ggtttgcgag tcgctgtaaa
    86041 tgggcgtgtt cagtccttgt tttttggtga gggccagcgc gtgtacgatg gctaagaact
    86101 cgccgatgtt gttggtaccc agcggaaatt ttttgtaaaa caactgctcg ccggtacggg
    86161 tattcacgcc ctgatattcc atgacgccgg ggttgccgct acaggccgca tccaccgaaa
    86221 ggctgtccca gataacggag gcgttggaag gcatgccggc gggcttgggc gaggacttgg
    86281 actttgcacc tgcggggctt ccgccaatgt gccgggccgg accatcaagg aaggctttgc
    86341 gggcttcggc ttcggagtcg aaagatttgt acaatgcttt ggcgaatccc atggtctgag
    86401 cctgacattc gttccaagaa ccatatacgc cgggctggtg tcctttccag acaacgtaga
    86461 acttctgctt tttagccatt cagatgaatt ggtatagttt attttaagtt tccgtgagta
    86521 gcgaaggggt gactacaagt gttgcagagt caccccttcg ctcatcttcg ccatgccctc
    86581 aattccagtt ggcggtatcc ggcatggtag cgatgacgga atacacatcg cctttgttgt
    86641 gcatcacctg ctgccaaagc tcgtccgcct gggttttgaa cacatagttg gggtgcatat
    86701 cggcggtgac ccaggaaccg gtggccattt cgtccatgag ttggccctcg tcccagccgg
    86761 agtatcccac aaaaaagcgg atgttgtcgg gtgtgaccaa tcctgaattt atgaggaatt
    86821 tgagcttatc aaaatctccg ccccaataca cgccttccgt taccttgacg caatcttcca
    86881 gcagatcgcc cagattgtgg aggtagtgaa tggtgttcat ttccaccggc ccaccctgat
    86941 aaacgatgct gttgaattcg ggaaaatcct ccagtaattc atcaattcta tactctgtag
    87001 gccgattgat gacgaatccc acgctgcctt ccgggctgtg ttcgcatagg agtacggttg
    87061 ctctgcggaa gttggggtcc aacatgaaag gctctgccag tagtatgatg ccgtttttga
    87121 tttcgtaatt taccattgat cggatgcttt gctccaaaag tgcagtattt ttctcatacc
    87181 gcgcaagttt ttgtatataa atttcttcct gccgcagcgc tgtcggcttg ggctttgaat
    87241 gacagccgta aagccgacag ctccgcgact tacagcgcgg aagtggcaaa ggccctgtcc
    87301 aagcgcttca tcatcccttg caggactttg cccgtaccgc ccgattcgat catttcggtg
    87361 aagccgtggc ggatgaagtg ctgcatggtt tgcgtccagc gcacaggggc ggtcagttgg
    87421 ttgatgagat tggctttgat gacctccgga tcggtggcag gcaatgcgtt tacattttga
    87481 tacaccgggc aacggggcgc ctgaaaaggc gtggcggcaa tagcggcctc caactcctcc
    87541 tgcgcgggct gcatgagggg cgagtggaaa gcgccgccta cggccaacat ggttactttt
    87601 ccgccggctt ccttgcactt ttcggcggca gcctctacgc cggcctggct gcccgaaatg
    87661 accaattggc cggggcagtt gtagttggcc ggcaccacga tgtcgctcac cgaggcgcaa
    87721 atttcctcca ctgcgtcgtc gctcagcatc agtaccgccg ccattgttcc gggcacggct
    87781 tcgcaggctt tttgcatggc aaatgcgcgc tgctgcacca aacgcaaacc atcggcaaac
    87841 gacaaagcgc cggctgccgt gagcgccgaa aactcgccca gagagtgccc ggctactgcg
    87901 tcgggttgga aatcgtctcc caatatgagc gctttcacca cgccgtgaac gaacagtgcc
    87961 ggctgcgtga ttttagtctg gcgcaagtct tcgctcgtgc cttcaaacat cacatcggtg
    88021 aggcgatagc ccagcacctc gttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    88081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    88141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    88201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    88261 nnnnnnnnnn ntggctcttg gttggaagct gttttgtggt tattccgtga aatcgaaata
    88321 ctcgattttt ttgacaatgg tgttgtaaat tttccaaaga ccgccggcct ttctgaacag
    88381 ataaatctgc gtggtttcgt tctcgcggaa cggcacccct tcgatggcgt tgcgggtaaa
    88441 ctgcacgacc tccgcgccgg ccatctcgtc ggtttttgac agcagacgaa tggagcggac
    88501 ttcgggcagg gcgtcaattt gctgaaaaat ttgcaggtag gtttcctgcg tttgctcccg
    88561 gcccggcata tcgggatgga aggtcttcat aatgccgtca atgtcttttt ccagcatggc
    88621 gtgcatgtgt gcatacacca aactctctat ggccggatcg cgcatattgg cctgctgttg
    88681 ctgccactct ttttctaaag ctttcacctc tttggtgatg ttgagcaggg aaaatccgag
    88741 ttcgttgtgc gttttgcgag cgtcctcttc gctcagaatt tgcagcacat agtagtcgtt
    88801 ggccagcttg ttcaactgca tgatgaccat cgtgatctgg tcgtccagat cgggacgatt
    88861 tttgtctttt agaaactcct gaatggtagc ggcggcctcc tgcaactgtg ccttggccag
    88921 caaatcggaa atgatgaatg agatgccctg cgtcataata atatggattg tttagaccaa
    88981 tatttcctgg cctgcatagc tgtgttcaat ttcatccaaa acagccatca gttctgccgg
    89041 atcggagatg gaaacgagcc gggtgcgaaa cggtttgatt ccgtgaaaac catgaaaata
    89101 aggactgtaa tggcgccgca tttccaatac gccgagtttg acgcctttcc aggcaatgct
    89161 gtcgcggagg tgttggcgca cggcctccac gcgctcggca atatcggggg cgggcagcag
    89221 ttcgccggtg gcaaaataat gcttgatttg ctgaaaaacc cagggactcc cgatggcgcc
    89281 cctgccgatc ataatgccgt cggcgccata gcggttgcgg tattcgaggg cgcgttgcgg
    89341 ggtatccacg tcgccgttgc cgaagatggg aatgtgcatg cgcggattgt cttttacttt
    89401 ggcaatccag gtccagtcgg cctcgccctt gtacatctgc ttgcgggtgc gggcgtgtat
    89461 ggtgagagcc tgaatgccgg catcctgcaa gcgctcggcc acatcgagga tgttgatgct
    89521 tttttcatcc cagccgaggc gggttttgac ggttactggc ttggaggtgc nnnnnnnnnn
    89581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    89641 nnnnnnnnnn nnnnnntact ttggccaccg gacaaccgaa attgatgtct atcacatcgg
    89701 ggttggctgc ttccacgaag tgtgcagcgc ggatcatgct gtccaactgc ccgccgaagt
    89761 attgtatgcc gatggggcgc tcgtagtcaa agatttcgag cttttggaag ctcttgtccg
    89821 attcatacac caggccgtcg gcgctgatga actcggtgta catcatatcg gcgccgtaac
    89881 gcttgcaca
//
LOCUS       DAORLF010000002        41904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_478_length_41904_cov_2.241248, whole genome shotgun sequence.
ACCESSION   DAORLF010000002 DAORLF010000000
VERSION     DAORLF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 41904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41904
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_41904_cov_2.241248"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="PK198_00540"
     CDS             <1..1986
                     /locus_tag="PK198_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF37247.1"
                     /translation="RDDKEGRFLLAVANYHLNLLDESLQQLRALGDQDRYPFPECRLY
                     LGKIYHARHEFAEAGRQYKKYLKTLSASSPLRRAVWNEVKRCNNGMELQFKTSPVFVE
                     NLGAQINSSDDEFGPVPSPTFMDRLYFSSIRKGNAGGLRDERGNPDDLKGRYFSDMFS
                     ARLSGGQWGQVQALPYHLNSPRHEVLADINALGNAMFYFKGLSLQKGEILIDTFRQGG
                     ASVLTSDPFIGPLNPLEGEVSFHLFNDTLLIFSSARAGGFGGYDLYRSSKINGRWNTP
                     ENLGPEINSAFDEATPCLLRDGRTLFFSSNNSSGSIGGMDIFKSVWNPRIKKWAAPEN
                     PGIPINSAADDTHFRLSRDGFTAFFASSRKDGFGMRDLYVAYFNDFLELEPMTTTTYT
                     PPQPAPNVTTGTQRIAVSEPVSVTVVPAKTTPAVQEEQPRAAATWPVLKKAELLKTDG
                     NAHAAAIKAAADYLKANPALRLSLTAFVNSPEAPGAALFNGIGHAEAAAARMVRMGVS
                     PDRIFVRAASAKWDEPTAGASVLLMRWTPESGQSAPEYVSLQKQLPQAVYNAALEAPL
                     LYKVQVTSLKSAYAGDLLTKFPDGMVEKSHGVDFYRYTLGAFTTFSAADQFKKQLAAR
                     GQTNAFITVYVHGFRTERSAIQSYVSDFPDLRNYLGN"
     gene            complement(2001..>3614)
                     /locus_tag="PK198_00545"
     CDS             complement(2001..>3614)
                     /locus_tag="PK198_00545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF37248.1"
                     /translation="SALTEDEAYYWMYSTQLNWGYFDHPPMIALFISAGKWIGGELGV
                     RMVAVLAQVGALWVLWRLTCEHWPGPQRPLLFFGIAFSIVIFQVFGFVATPDSPLLFF
                     AALFLYTYRRLVLYNRWSDALLLGALSAGLLYSKYHGVLLIGFTVLSHPRLLLNPRLY
                     VAGLTGAVLFFPHLWWQYINDFPSFRYHLVERTQEFKWWYVSEFLLNVFLVYNPLLWP
                     MAWRAWRHSNWKDPFTRALAVNFAGFLLFFFLSTFRGHVQPQWTVISSVPMLLMLYAH
                     SLHDARRTRYLLRVAVVVLPLVLVARSLLIGEWAPLPNQFFRPEAFTKKTEALAAGRP
                     VAFSRSYQRASLYAFYTGQLPAFSAGTYKSRRSQFDLWNGEEQLHLRPVLWVGGQSNP
                     DPAFFMLDRDTITYTEIDTFYAFYKVQPVADALPAELKAGQEVRLSLHLYNPYPYELD
                     FSKIARLQIMAVWMDGKKLGCFVPLQCRLPHLTPGASATLDAGFIAPDELPAGRYRVG
                     FSFQYEDIMPFPAGNWQMLRLGTGSAEKR"
     assembly_gap    3615..3642
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3776..>4886)
                     /locus_tag="PK198_00550"
     CDS             complement(3776..>4886)
                     /locus_tag="PK198_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF37249.1"
                     /translation="EMLSNIFNKEAWRPLPQGDGQRLSIRAQTNGNFYQSYNISLTDP
                     WLGGKKPNSFTVAGFYNRYSFTANSSFSILNGSASLGTRLKWPDDNFLSTTALSLQTL
                     SLNDWSAGALGSFSDDNGNLITDGKFNNFSLRQTIARSTVYDPIFPREGSRISLAVQL
                     TLPYSLLNGKDYTDLGPEERYRWLEYHKWRFDAEWYTPIVGKLVLKASAKIGILGSYN
                     RKVGTVPFERFQVGGDGINNQQFGFQGVDIISMRGYEVSDLSANFDAAERTSPAPIFD
                     KFTLEMRYPLSLNPSSTIYVLAFAQGGNAWRNLRDFNPFDLKRSAGIGMRVFLPMFGL
                     LGFDYGIGFDKQRLPGERGLKGLGDFNIVLGFEPE"
     assembly_gap    4887..4896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4897..>6246)
                     /locus_tag="PK198_00555"
     CDS             complement(<4897..>6246)
                     /locus_tag="PK198_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRF37250.1"
                     /translation="NLPVYPFGEPKEFEIGGIKVTGATYSEDVAIIGVSGLKVGDKIR
                     IPGPEIPRAMKALMRLRLFTNVQIVLDKVIGDVVFLEIQLQERPRLSKYAYRNVPKTY
                     HENLNEQVNKFMLKGGIVTEDVKLNAQRAIEKFFIDKGFFDAKVKVMEEPDTARVNSV
                     IVYFDTDRGERIKIEDIEFAGAQNAKEKKLARKMKETKEKGKLFGSSKLVRKDYETDK
                     EALVKYYNTIGYRDARILGDSVWRDEDGRLRVKILIEEGQRYYFRNIVWKGNSIYDNS
                     TLQGILGIEKGDVYNQELLEKRLRFSQDGRDVSTLYMDNGYLFFNVDPVEVAIEGDSI
                     DLEMRIFEGPQATIDKVIIKGNDRTHEHVIRRELFTRPGEKFSRSDILRSQRQIVNLG
                     YFNPETLGINTPVNPQRGTVDIEYTVEEKPSDQLELSAGWGGIQRVIGTLGVSFNNF"
     assembly_gap    6247..6352
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6493..7257)
                     /locus_tag="PK198_00560"
     CDS             complement(6493..7257)
                     /locus_tag="PK198_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRF37251.1"
                     /translation="MQAVRMELKSAIILEKLPRHVAVIMDGNGRWAKQHGKPRVFGHR
                     NGVQAVRDITEAAAELGIEYLTLYAFSTENWNRPKIEVGALMRLLVETLHKEVKTLMK
                     NNIRLMAIGDLSQLPDATRRALLQGIETTKNNSRMTLVLALNYSARREILQAARLLAE
                     QVQAGELKPEEVSEERFAARLNTGMNQIPDPELLIRSSGEQRISNFLLWQIAYAELYF
                     TPVLWPEFRKEHLYQAIIDFQQRERRFGKTSEQLAQ"
     gene            7377..>8377
                     /locus_tag="PK198_00565"
     CDS             7377..>8377
                     /locus_tag="PK198_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRF37252.1"
                     /translation="MRFEQLQYQRPDMAQFSARFYELLERFSNAESFDLQSEAFTKIN
                     ALRDEFSSMFNLCHIRHTVNTKDEFYATENDFFDQEMPNVEALNFKFYEKLLASPFRS
                     ELEAKWGRQLFQIAELSRKTFSPDILEDLQKENALVSAYVKLKAGARITCLGEEYNLS
                     SIIKLENSPDRGVRRAAAAAKWAFFDEHAAEVDDIFDQLVELRHGMAQKLGFHNFVEM
                     AYARMLRSDYDAQMVAGFREQIRQSIVPIATRLYERQRERLQLERLHFYDEDYRFPDG
                     NPHPKGDPEWIISQAAQMYDELSPDTSEFFRFMLDGGFMDLPARDGKSPGGYCTFLNS
                     "
     assembly_gap    8378..8470
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8471..9057
                     /locus_tag="PK198_00570"
     CDS             <8471..9057
                     /locus_tag="PK198_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRF37253.1"
                     /translation="SSRNIGINEYYWPSYEACEIHSMSMEFFTWPWMRLFFGEDTDKY
                     HFAHLSGAIYFLPYGVAVDEFQHLVYENPQWTPAERRQAWRDTERKYLPWRHYDNNAF
                     LESGGYWHKQSHIFSTPFYYIDYVLAQICAFQFWKRDREDHTSAWKDYVQLCRAGGSG
                     SFLEMTRLANLRSPFEEGCVDSVSGVVAEWLEGH"
     gene            complement(9069..10139)
                     /gene="prfA"
                     /locus_tag="PK198_00575"
     CDS             complement(9069..10139)
                     /gene="prfA"
                     /locus_tag="PK198_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRF37254.1"
                     /translation="MTEKLEAIKERYIYLEEQMADPGVIADSDRYRKVSKDYKNLKPI
                     VHAFNEQLDTRAAIASVKEMLRDEDPEIRAMAQEELAELETRLADVDERLRVLLIPKD
                     PNDDKDVLFEIRSGTGGDEASLFAGDLYRMYQRYFDAQGWKVEVLDVNEGTVGGYDRL
                     ILEITGDEVFGKLKFESGTHRVQRVPKTESQGRIHTSAATVAVMPKLEMEDVNINKAD
                     LKVDTFRASGAGGQHVNKTESGVRFTHLPSGVVTESTEARSQHQNREIALQRLYQRLQ
                     EMEYEKVHSEIASRRKSIVGSGDRSEKIRTYNYPQNRLTDHRINLTLYNLDAVMDGRL
                     EEIIEALQIADTAEKLKAEAEG"
     gene            complement(10337..10726)
                     /locus_tag="PK198_00580"
     CDS             complement(10337..10726)
                     /locus_tag="PK198_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRF37255.1"
                     /translation="MRYLIDSHALIWFLNGDNQLSANALAILSNSDLDKYVSIAALWE
                     IGIKINLGRLHLSCPFTDLPAFLAAHDFQLLPTNFQHILSACFLPSHHADPFDRMMIG
                     QALCENITVITKDPSFPLYGVATVWQE"
     gene            complement(10726..11034)
                     /locus_tag="PK198_00585"
     CDS             complement(10726..11034)
                     /locus_tag="PK198_00585"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HRF37256.1"
                     /translation="MFELILRTDNQAALQKLLEYIDSLNFQIVKEPASQEKKITIQIA
                     EGDSMMPNGDNREEKIVEWGGMKVDISGITPGFGCAKGMFEMMPDFDEPLEGFKEYMH
                     "
     gene            complement(11119..>11856)
                     /locus_tag="PK198_00590"
     CDS             complement(11119..>11856)
                     /locus_tag="PK198_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRF37257.1"
                     /translation="PRMGEVVRAASLSQYEKIPVEKIMGAVVVDRIMDVLCILSITAI
                     ALVVEAGPIWGFFSKNVPVADLLGGLGNLALWGFGTLAVVVLAVYLLRRQLKSSRIYQ
                     RIRHIAIGFWQGINTVRSLKRPSLFLVHTVNIWVMYFLMTLLCFAAFEPTAHLTWRTA
                     LTVFVFGSWGVVVPSPAGMGTFHFMAQQALGIYGVGGDDSFSWANISFFSVNLGANVL
                     IGLLSLLLLPIINRHYEPKKSGETESE"
     assembly_gap    11857..11896
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11897..12194)
                     /locus_tag="PK198_00595"
     CDS             complement(<11897..12194)
                     /locus_tag="PK198_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HRF37258.1"
                     /translation="MYRTYIIQFFKFLLFLSIGLGILYWVYRGQNIAFQAECGAKGIA
                     PEDCSLARKVIADFGRVNYAWIALVLAAFIVSNVSRAARLNMLLHPLGSRPRLIN"
     assembly_gap    12376..12389
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12390..14684)
                     /locus_tag="PK198_00600"
     CDS             complement(<12390..14684)
                     /locus_tag="PK198_00600"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF37259.1"
                     /translation="MVSKWKSLKLCLSVLLCSIGSVLWGQASIAGVDFALLSGFYETP
                     VTVGLSASEGARIYYTLDGNKPNAQAHRYTRPFLLKKSAVVRAVAVYPNGEASRPDAQ
                     AYFIGEPKSSLPVVSVAVPPGLLFDPDIGLFMTGNRLDTGSIMQEGANFWSRRELVAH
                     VEFFETDGSSVFNSQMGMRLFGGMSRLFPQKSLTLAARERYGEKRFHYPIFGEDGPKS
                     CKFLVLRNAGSDFGRAHFRDALMSGLLDDWDLEKQAYRPAHVYLNGEYWGIYNIREKI
                     NRYFIADHQPVDKDSIDLLEHRDNLRRGKKNHYLRLLRFLQSRNMAEPANYAWVQSQM
                     DVDNFMNYQIAQIYFDNQDAGGNIKFWRPQTPEGRWRWILYDTDWGFGLHDPKAWRNN
                     SLAFHTEPDGPHWPNPPWSTFILRKLLENPDFRQAFTLRFCDHLNTTFHSERVLAAIE
                     EKYRTLLPEMPRHLQRWRLSEAKWERQVQIMRDFATYRPNHVFQHLSNRFHTGPLREL
                     RATASVGGTLLVNDHIEVKASEGDFSGRYFERQALHFKAIALPGYRFVRWEGDAEGIG
                     SREIALPMSKEGISLRAVFEPYSHPLANKLIINEICPLNGQAGDWIELHNITKERIRL
                     DGWIVADARNEALLPAAVIGPKDYLIICRDEARFRKVFPQAHNVMGGMDFGLHKREDR
                     IAVYSADGALIDEIQYQLPPTDSAFTLALLMPHLDNNDPENWELKPGLGTPAGANPFF
                     VQSRIDRIQREWLEIGIAAGGLVAG"
     assembly_gap    14758..14775
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14809..>15175)
                     /locus_tag="PK198_00605"
     CDS             complement(14809..>15175)
                     /locus_tag="PK198_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF37260.1"
                     /translation="SGKITVLSFWATWCKPCQQELDAISEVYPEWQEKYGVQLVAVTI
                     DTQRALAKVGPMVESKGWEYIILSDPNGAMPAALNFQSIPQTFLLDAKGNVIYEHTGY
                     IPGNEFDMEEKIKAAAGKK"
     assembly_gap    15176..15195
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15448..>16185
                     /locus_tag="PK198_00610"
     CDS             15448..>16185
                     /locus_tag="PK198_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRF37261.1"
                     /translation="MKRLGHLLCTLICAFVAIAPLAAQDNNSGGKISGSLQLNANFFM
                     RDSLIGAANTPQYDRQLYGGESWLNLAYSNWGFDVGLRFDLFNNSNLLNPTGSYSGEG
                     IGRWYIQKKIDKLGIYGGYIYDQIGSGIIFRAFEERPLLIDNALFGLRLSYDLHPNWK
                     IKAFTGRQKQQFSTYNATIRGISIDGFIAAKDSTSSFSLAPGFGAVGRTLDDESMNSL
                     VAILNTYPQDDAFTPKYNSFAFSAFNTL"
     assembly_gap    16186..16283
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16284..>16775
                     /locus_tag="PK198_00615"
     CDS             <16284..>16775
                     /locus_tag="PK198_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRF37262.1"
                     /translation="TGDKFIFKEGTVLYSSFSYAAKGLGISLEGKRTENFSFRTRPQE
                     QLNRGLVNFLPPMARVNTYRLNARYNAATQELGELAVQADIRYAPSKKIAFNLNASNI
                     TDLNDVLLYREFFFDVTFKKDRIWTLTTGVQHQNYNQERFEFKPGVPNVQTITPFADF
                     LYKF"
     assembly_gap    16776..16808
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16809..17151
                     /locus_tag="PK198_00620"
     CDS             <16809..17151
                     /locus_tag="PK198_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRF37263.1"
                     /translation="ETQYMHTGMDEAAGYKQDYGDWLFGLVELNLAPRWSFVVSDMYN
                     AGPGKNSPADENGKKLKLHYPRFDVFYTLKSNRISLSYIKQVEGVVCTGGICRLEPAF
                     SGVRLSVSSTF"
     gene            17235..>17925
                     /locus_tag="PK198_00625"
     CDS             17235..>17925
                     /locus_tag="PK198_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRF37264.1"
                     /translation="MFLAQLLYYIGALLSVPLLPVLYFQGKKLRKNFPRLPEPEHNVQ
                     GRTEEGVGAPIRILALGESTIAGVGVQDHRHGFTGELAAALSAHTGRPAEWQVVARSG
                     YDAKDVFRKLTPKIPDAPYDLIVIGLGGNDTFYLNSPLHFRHNMAHLVRHLRERMPDT
                     PIVIAALPPIGVFPALPGLVRSALGALVHLHGFAIRNIPRRFANVHYPGGPIRARRWM
                     DDIHSPEQFFSD"
     assembly_gap    17926..17935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18038..18604
                     /locus_tag="PK198_00630"
     CDS             18038..18604
                     /locus_tag="PK198_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37265.1"
                     /translation="MRYQLAAIGLIIGILLPSASAVLMYRVARSQIRSRIEEQIAAGK
                     AEDGLVLLHIPRRLEDQPNADFRRFHATEFRYKGIMYDVVRQEAKGDTTLYWCIADIE
                     DTQLQNQWDNYWARGSRSDNPAQRAAEDRLFQFWQSLFVHSLPPVNHLFGSGASSKPR
                     PCANKYSSVSRPIPQPPPEPLFPVLVQC"
     gene            18747..21146
                     /locus_tag="PK198_00635"
     CDS             18747..21146
                     /locus_tag="PK198_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37266.1"
                     /translation="MRNLYFILLCLVCMASTSVYAQTLIVQQAGTRQALEGVTITSAS
                     ANAVTDAAGKADISTMKSDTGEIQIRSVGFETLRTRYALLEKQGFMAIMQEDRISLDA
                     VVVSATRWQQSRRETPLRISTIGAKEVMLQNPQTAADLLSLSGEVFIQKSQLGGGSPM
                     IRGFATNRVLLTVYGVRMNNAIFRSGNLQNVISLDPFSIQRTEVVYGPGSVIYGSDAI
                     GGVMHFSTLEPAFSSVNANAAVRYASANNEKTGHADFSVGGKKLAFAGSATFSDYDNL
                     LMGKNGPDDYLRPRYVIRINGRDSVVANSNPRLQVPSAYNQFNWMHKLRWAPGEKWEL
                     QYGFHYSATSDYSRYDRLLRPRGNTLRSAEWDYGPQIWMMNNLSAAHHSESKWFDNVS
                     FRVAHQFFEESRIERNLNSLTRMIRTEKVHALSANVDFEKTRQNGQRFFYGLEAVYNK
                     VGSTGEDVNIETELRRAAPSRYPDGALWYSAAAYLTWKAPLSEQLTLQTGARYNIAGL
                     QAEFDTTLFVLPFAEARQASGALTGSVGVIWSPTALWLLSANAASGFRTPNVDDVGKV
                     FDSSPGFVVVPNADIRPEYAYNFDLGIARTFGSTLKVDVTGFYTLLDNALVRRNFTLN
                     GQDSVLYGGEMSRVQAIQNAAVAKVWGLQLGLELKLPAGFGLRVHHNFQKGEEELDNG
                     SVAPLRHAAPSFGTTRLTWSNRKWQAELNLIYNAEVANADLAPEEQGKEYLYAQDAAG
                     NPYTPAWHTINLKTSYQLGNRANIGFGIENITDQRYRPYSSGITAPGRNFVVSLRVKA
                     R"
     gene            complement(21152..21283)
                     /locus_tag="PK198_00640"
     CDS             complement(21152..21283)
                     /locus_tag="PK198_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37267.1"
                     /translation="MPGVSSIVFSGENKKVVAGTLYSTPATSEIFIVFSDEAPLLSG"
     gene            complement(21620..22450)
                     /locus_tag="PK198_00645"
     CDS             complement(21620..22450)
                     /locus_tag="PK198_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF37268.1"
                     /translation="MKKILIPTDFSPCADTAARIGRWIAKKVNAEILYLHGLKTPVDW
                     VNLPKRLEDTYPEIKAQIGAAHQKLDELVLATEHEGLKAEKSLAFLEGFETIANTVLD
                     TQNDLIVMGSHGLTGVKKFALGSHTEKIMRTAQAPVLACKKPGDELKFNTIVFASGLE
                     ADTHPAFDQLLRFADSMNAENLYFVEVTTPYNFRPTGEVMGKIRDFVDAHDFRHISLH
                     SYAHYTIEAGILEFAQSVGADLIGIANHGRTGLGGIFVESIPENLLRYGQLPVLSIRV
                     "
     gene            complement(22469..22915)
                     /locus_tag="PK198_00650"
     CDS             complement(22469..22915)
                     /locus_tag="PK198_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF37269.1"
                     /translation="MWRAEIKNHAFERFGEEFHPVVDSNHFLGRSSFDIPYQQSTPPA
                     NLRKDGDLFELELAVPGFTKEEIEITLKDDVLVVKGEKSRAERRPGAEFIMEEFDFDS
                     FERAFKLASGIAHEQIAASCEHGILKLSFTHTPVEDERAYRKIKIE"
     gene            complement(23030..24583)
                     /gene="rpoN"
                     /locus_tag="PK198_00655"
     CDS             complement(23030..24583)
                     /gene="rpoN"
                     /locus_tag="PK198_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRF37270.1"
                     /translation="MLKQSLSQKMLQKLAPQQIQLMKLLQVPTAILEQRIKEELEENP
                     ALEEGDYSEDSDDSTEDEMYDEDGGYEERSSDDREGDEVSPDFEKEKDNPLEFSDYLN
                     EYIADDEASYKTRGDAYNADEEQRTLPVAIESTFHDYLEQQVGMMELGGPTQEAIARQ
                     IIGSIDDDGYLRREIDSIMDDLLFSQNISATEAEIKAILKKIQRFDPPGIGARDLQEC
                     LLLQLEWLLEQEHLKEEDRRPIQLAARILSRHFDEFTKKHFDKLQRALDVSEEELKEA
                     IDEILRLNPKPAGAFVGSSSSTQYVVPDYIISNNDGELELTLNARNAPDLRISDHYKD
                     MLRDYKKAAQGRRPNVREKEAILFIRQKIDSAKWFIDAIRQRQDTMYRTMYAMMQYQY
                     DYFLTGDDRKLRPMILKDVADITGLDISTISRVVNSKFVQTEFGTRRLKDFFSESLQT
                     SEGEEVSTLEVKKILSDLISAESKRKPLSDEKLTELLTEKGYNIARRTVAKYREQLNI
                     PVARLRKEL"
     gene            24889..25479
                     /locus_tag="PK198_00660"
     CDS             24889..25479
                     /locus_tag="PK198_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRF37271.1"
                     /translation="MEIIAINGQPRTDLGKKGAKAVRNGELIPCVLYGAGVSEHFSTE
                     VSSVRHLIYTPDFKVAEITVEGKKHRCIVKAVQYHPVSEKIMHIDFLSLVPGHPVKVE
                     VPLRFTGSSIGVKGGGKLQQNVRRVKIKTMPEDLVSELKLDISNLDMGQSVRVRDIKA
                     PQNVEILTAASTPVATIEIPRALRSAAAAADKPGKK"
     gene            25589..26299
                     /locus_tag="PK198_00665"
     CDS             25589..26299
                     /locus_tag="PK198_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF37272.1"
                     /translation="MLRTFSFLLLLTLLSSCTASELQQLLGSGELPLTQAEAASGLKE
                     ALSIGIGKGAELLSQKDGYFKSEYKILLPAEARKVTEKLKVVPGFSNVEEIILERINR
                     GAEDAAMKAKPIFLNAIKEMSFTDAMNILLGGQDAATRYLESATNEQLYQQFNPVIIE
                     SLDRLNARKYWSDAVMAYNKIPFVDRANPDLDDYVTREALKGMYGMVAKEELNIRTNL
                     SARSSDLLKKVFAKQDKK"
     gene            26478..>27242
                     /locus_tag="PK198_00670"
     CDS             26478..>27242
                     /locus_tag="PK198_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF37273.1"
                     /translation="MYIRHLLLTASLLCISLSLSASGIAGQVTGNSGEALPFAAIFVK
                     ETGSGTSANEDGYFELRLEPGRYTLVFQFLGYETVSRTVDVRSGPFTMVNVALKQQAV
                     QLRTVEVSSKQEDPAYTVMRKAIAKAGYHRQQIERYTAQVYIKGSGRLKKAPFFLRKE
                     LKKEGIDSSRAFVSESVSQISYQRPSTFKEKVISIRKQGNDNSTSPAPFINGSFYEPE
                     IAEVISPLSPKAFAYYKFKHAGYFMDRGYGVNKIQVI"
     assembly_gap    27243..27318
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27319..>28076
                     /locus_tag="PK198_00675"
     CDS             <27319..>28076
                     /locus_tag="PK198_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF37274.1"
                     /translation="YSLDLKTYKLGIGIDVAQTYAPIEDKAWLPIKHQIEVDGTFFGF
                     QFEYKYIATVSGYKIELNPALDVDFKVLDEKIAAPELVQTAKKQQTVAKKSAAEQRLA
                     TGEELTMKDLRKIVRDYEKEDRKKAVTEDKDSLAGFASVTEYKIDSLAEQRDSAYWAE
                     IRPVPLTLYEVRGYQHVDSIAVVEADEKTERDSLGIAPGEDEEVEKKRDKFKPQELVL
                     GGSYRLGKRSRLSVGSVLANLNFNPVESFHSWSDL"
     assembly_gap    28077..28262
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28263..29165
                     /locus_tag="PK198_00680"
     CDS             <28263..29165
                     /locus_tag="PK198_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF37275.1"
                     /translation="NPISSIMNTAYNLLQERNYIRLYEKEYFKVQVNHQLRENISFQA
                     GGEWARRFFPDNRTTQTWFKRREGGYESNIPVNEEWAGPPAEPEKAFVLHATVEARPW
                     QKYRIRNGNRQAIEDSSPTIGLSYRKGIPGIGGSVTDFDHIDLSFRHSFRPGARGKID
                     IKAEVGAFLNNNYVGFADYRHFMGNRIPIVTTDPVGSFRLLDYYRHSTSEEYAALHLH
                     YQFRKLLLTQIPKVWLTGVKENLFVNYLATPTSKHYTELGYSLDNILRIFRVETAVAF
                     QNGRYYDWGIRIGVASNLGFIRFD"
     assembly_gap    29338..29348
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29349..29668
                     /gene="rhuM"
                     /locus_tag="PK198_00685"
     CDS             <29349..29668
                     /gene="rhuM"
                     /locus_tag="PK198_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HRF37276.1"
                     /translation="LNAFREGELERSAVVAKFATTASDGKTYQVEHYNLDVIISVGYR
                     VKSKRGTQFRIWATQVLRKYLIEGYALNEQRLREQEQNLENLQEAVHLVAQVVVNLIN
                     AKN"
     gene            complement(29877..30614)
                     /locus_tag="PK198_00690"
     CDS             complement(29877..30614)
                     /locus_tag="PK198_00690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HRF37277.1"
                     /translation="MAKLIIFLSIQLMVIAPLVALGLNKPDYKWKTLILFVIYYFFYA
                     SFLSLPFLFPETKIIQSDNWNWSGKILAIAGSLLFYWLFKKTFTDIDYITFKQRKKSL
                     KPALFITIFVFLLAIGLAFLCVKKSDEKLEYFLFQFTMPGIDEELAFRGIMMGLLSNS
                     LKSKIKIGSVYLGNPALLITSILFGLGHSLQIDNDWTVHQNWFEFFNTFAIGLLLGWM
                     TIKSGSILMSTLTHGLINVLPKIIFWI"
     gene            complement(30760..31878)
                     /locus_tag="PK198_00695"
     CDS             complement(30760..31878)
                     /locus_tag="PK198_00695"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF37278.1"
                     /translation="MTRTILTLCMTLWALKPVQAQQVFPYAHQGKWGIVDKNGQVTMP
                     PALDSIGLFSLDNGESMHLAVARKGGKSGLITPAGAWVLKPSVDSIMWLAQPRLRWAV
                     LGGKYGLLHVDGKKAKWLTKPIFDEVDFFAQGKKPLVRVRQGSGWGVLQTTGKLLVPC
                     VNEEVSIYSDFEQGLYVHITNKGKITRIADNGTPMAEEEAVYFAQDITDEDIEESWPK
                     RKVEYRTRVADLENLPSVVMLESSTDHSTWKIVKHVVIPQGCEVKHVEQDFGKIVHIM
                     IARSGKFGFVNEVGAFITEPIYDRVSWHKGNYHPPVALLYLGDRVGVAGQDGRQTLPP
                     QFSQIQVKGDRLMVSTPNGYQGWAEPTGEVHLPAGWRE"
     gene            complement(31958..33829)
                     /locus_tag="PK198_00700"
     CDS             complement(31958..33829)
                     /locus_tag="PK198_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRF37279.1"
                     /translation="MKQKVEILAPAKNLYQGMAAINAGADAVYIGAHTFGARTNAGNS
                     VEDIAELTKYAHLFKAKVLVTVNTILYDNELEACRQLIHELYEAGVDALIIQDMGILE
                     MDLPPIALHASTQTNNRDAAHVKFLADAGIRRVVLARELNLDQIREIHEATDVELEFF
                     VSGALCVSFSGNCYMSVANGERSANRGSCAQNCRLPYNLIDGNGTTLLTETHLLSIKD
                     LNLSDQLPVLIEAGIRSFKIEGRLKDLVYVKNNVSYLRKRLDAFLEDNPHYEKASSGR
                     TFYGFEAKMDKSFNRGYTDYFVNHRKERIGSWETPKSQGEYIGKLLETKANGYVIENA
                     ELLNNGDGLYFVNEEGRADGDRVNIIVNGLVVPNNFRPLPAGTDIYRNFDAEFNKLLE
                     REDSAVRKIGVVLHFAESLEGFTLTATDEDGHTSTATLTVAKERTKNGESIIDNIRKN
                     LAKAGNTPFIVDDIQVDFSDNWFLPISKINEVRRTVLEQLMDTRVREYHREERPIAPT
                     EHPYPVTELDFTYNVSNKLARQFYRRHGVQSIEKAFELQWDPGKSRVMTTKYCVKYEL
                     GKCPRFQRETMGEKVKEPLVLKHGEVEYKLKFNCKPCEMEIWEKDAEFEIEEEGIWD"
     gene            complement(33991..34146)
                     /gene="rpmH"
                     /locus_tag="PK198_00705"
     CDS             complement(33991..34146)
                     /gene="rpmH"
                     /locus_tag="PK198_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRF37280.1"
                     /translation="MKRTYQPSKRKRANKHGFRSRMASKDGRKVLARRRGQGRHKLSV
                     SDERRLK"
     gene            34304..>38425
                     /locus_tag="PK198_00710"
     CDS             34304..>38425
                     /locus_tag="PK198_00710"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF37281.1"
                     /translation="MKQLLTAVFMVYGALLAAQPVNDNCTGVLDLGIAPACPQTVFTN
                     VNATASDIGFGNNPSCFNGGIAPRDVWFAFTTNDTTLDYSITVTGLANGGFPALRNPP
                     IALYRGDCEVNGLAEIQCATSDPGETILRLDVYGLTPNVTYYVRISDYSPTATPNWGT
                     FELCVDPLSPVFNIDDGSSTACFGQLFDTGGPDADYSNNETHTFTICPSDFNSCITFT
                     LDYYNIESGDFGSPDQLSFFDAATADPGALITTIGGPGFPSDGGGGGVCFPIQASSGC
                     LTVQFRSDGSVTAEGFAGHWECSTVPCAPDSPITVQTGITEQDILNSITSLQTLVAVT
                     NINCPQSAYGTFQSTDNSLGLRRGLLLTSGDLNWAVGPNNDGGGGNLNTDNGAPGDPD
                     LDYLSVAFGDGTESNDACIIELDVTAFTNEITFEYIFGSEEYPEFVEDFNDIFAFLIS
                     GPGIAGDPNIGNQQNIAVLPDGSNTIVEINNVNNVTNWQYYRNNINGAATQYDGLTSD
                     LLGIKKSLTARASVIPCSTYHLKIAIADRLDFRYDSGVFLSEIRGGTPRLTAQFNSGI
                     DYLIENCTNTPDNVVVQLSNVLDETATYRVQVGGTAIRGVDYTLNLPDTVTFLPGQTQ
                     LSFPIFPLNDNLTEGIETIIIALTNDFGCGEVVYTTLTIELHDELVVNIIPDADTTFV
                     CQGATAQLDVEGAATYFWSPVNILDDPLSQTPVASPTVSGWVRVEGNVGICSDVDSIY
                     LQIIDPVISITALDPQLICQGDSVRLSVVDNVNNTNLIWTPATGINDPNSPNPVVTPQ
                     ETTTYIATVRLFECAVSDSITIDVNTFEFPQIGNDTLVCQNYGVQLAEMIDAVATTYS
                     WTPTTGLSDPTASGPIATPNVTTTYTLIATSQNGACADTAQITVTVVPADVDIQQPDT
                     LEICLGTVVPMTAVTSTGNAQGLLWSTNLPGTLSDTVGLNVVVTTSQTATYYTRFEVG
                     ACVVFDSVYVRVDSLPFDRAIIADPAKDPYCPGDIVILRSAVFEPSDFPDIEFQWLTA
                     PGFETPDTLWNMVISAIDTAIYRRVITNRGCLDTVSITINVVEPPTPRIIPSDTLICS
                     GEAVQLLAVDYGDYPISWTPTAGLSCTDCPNPIATPNATTVYNLQVDVPNCPVGASAN
                     IVVIPGPTVRMNNDQTICRGASVQLNLDSDPFTQYQWASNVDAGFSSADPLLVATPLV
                     TTTYTLTATRQNCPPRVRSITITVVQPPTLMASQDVTVCRGESTTLTATIVPANAGGA
                     FAWFAPGSGTPAGTASSLNVNSANLGAANRYIVTYAYGPGNSCGPVSDTVMVTVADPV
                     RIDSITSNVPDTTRLVIGNAITLTAHTTPGDPAGAMYFWSQDGGSEQMGGPVFT"
     assembly_gap    38426..38482
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38483..38815
                     /locus_tag="PK198_00715"
     CDS             <38483..38815
                     /locus_tag="PK198_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37282.1"
                     /translation="DGCISTRTYTREVIVPDIAFPNVFTPNGDDLNDLFRPLPTQVSG
                     VLIIEDFKIYNRWGQVVYNGKSNDNIGWNGEHNGSPAPIDVYVYRVKLRYPEGPLFEK
                     SGDLTLIR"
     gene            complement(38994..39488)
                     /locus_tag="PK198_00720"
     CDS             complement(38994..39488)
                     /locus_tag="PK198_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37283.1"
                     /translation="MNKILIILICLLNLQAFGQNEWESWDKNYTEVSLTDICNAEKQY
                     AERVEQDKNIPQYYARIDKYKIKAKYLGEFKKLDAQVLNSMKNVFTLYIGNPDQLNEM
                     VKNKCLFEIEGYRIWLPVQKVLEKSLKKELKNGDNVTLYCLFMNEHSHDGHLSNIFFV
                     SEFR"
     gene            complement(40051..40812)
                     /locus_tag="PK198_00725"
     CDS             complement(40051..40812)
                     /locus_tag="PK198_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37284.1"
                     /translation="MTRTKKIGIALTLILTTLLGLFYYSLENFFYSDLGCGCSSTCSV
                     YYPVLTSRLIGYFMMVSSVTLFIAGVWKLKRLSRWWTIPATLTFVIAFYGNGYSIFNK
                     GVCGYSINKAIFFVFQTKLGDFAKSDAETINLDSLDAGKYKGKLLGYSFNGDKLTIFK
                     IEDEPEVLKTRFLFWKIRNNTILHDISYGLNTFRNFEAEKKKGKYEFIGGKGMTEKDF
                     LDEFILTQKELTINLKNKKIINHSDGTTRFLFEKE"
     gene            complement(41049..41444)
                     /locus_tag="PK198_00730"
                     /pseudo
     CDS             complement(41049..41444)
                     /locus_tag="PK198_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024752.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         9658 a        10938 c        10477 g        10031 t
ORIGIN      
        1 cgagacgaca aagagggccg gttcctgctg gcggtggcca attaccacct caatctgttg
       61 gacgaatcat tgcagcaact gcgggcattg ggcgatcagg accgctaccc gttcccggaa
      121 tgccgcctgt atctcggcaa gatttatcac gcccggcatg agtttgccga agccggccgg
      181 cagtacaaaa agtatctcaa aaccctgtcg gcttccagtc cgctccggcg tgccgtttgg
      241 aacgaagtaa agcgatgcaa caacggtatg gagttgcaat tcaaaacctc gcctgtattt
      301 gtagaaaatc tcggcgctca aatcaattcc tccgacgacg agttcggccc cgtgcccagc
      361 cccactttca tggatcgcct gtacttttcc tccatccgaa aaggcaacgc aggcggcctg
      421 cgcgatgaac gcggcaaccc cgacgacctc aaggggcgct atttcagcga catgttttca
      481 gcccggctct ccggtggcca atgggggcag gttcaggcgc tgccgtacca tctcaacagt
      541 ccgcgccatg aagtgctggc cgacatcaat gcgctgggca atgccatgtt ttatttcaaa
      601 ggattgtctt tacaaaaggg tgaaattctg atagatacgt tccggcaagg aggtgcttcg
      661 gtacttactt ccgatccctt catcgggccg ctgaacccat tggagggcga ggtctcgttc
      721 catctgttca acgacacgct gctcattttt tccagtgcgc gggcgggcgg tttcggcggc
      781 tacgatttgt accgatcttc caaaatcaac ggtcgctgga atacaccgga aaacctgggg
      841 ccggaaatca attccgcttt tgacgaagct actccctgcc tgctccgcga cgggcgcacg
      901 ttatttttta gttccaacaa cagctccggc agcatcggcg gcatggatat tttcaaaagc
      961 gtctggaacc cgcgcatcaa gaagtgggcg gcgcccgaaa acccaggtat tccgatcaat
     1021 tccgctgccg acgacaccca ttttcgcctc tctcgcgacg gcttcacggc attttttgct
     1081 tcttcccgaa aagacggctt cggcatgaga gacctctatg tggcttattt taatgacttc
     1141 ctcgaattgg aaccgatgac gacaactaca tatacgccgc cacaacccgc tcccaacgta
     1201 acgacgggta cgcagcgcat agccgtttcc gagccggttt ccgtaacggt agtaccggcc
     1261 aagacgactc ccgcggtgca ggaagagcag cctcgcgcgg cagctacatg gcctgtattg
     1321 aaaaaggccg aactgctgaa aaccgacggc aacgcacacg ccgcggccat caaagccgct
     1381 gccgactact tgaaagccaa tcctgcgctg cggctttcac tgactgcttt tgtgaacagc
     1441 ccggaggcgc ccggcgcagc gctgttcaac ggaataggac atgccgaggc cgccgccgcc
     1501 cgaatggttc gcatgggggt atcgcccgac cgcatttttg tgagagccgc ttcagcaaaa
     1561 tgggacgaac cgacagcagg agccagcgtc ctcctgatgc gctggacccc ggagtcgggc
     1621 caaagtgcgc cggagtatgt gtcgctgcaa aagcaattgc ctcaggcagt ttataatgcc
     1681 gcattggagg ccccgctgct gtacaaggtg caggtgacct ccttgaagag cgcctacgct
     1741 ggcgatctgt tgaccaaatt ccctgacggc atggtggaaa aaagccacgg cgttgacttt
     1801 tatcgctata cgctcggggc ctttaccacc ttctccgccg ccgaccagtt caaaaaacaa
     1861 ttggcggcac gcggccaaac caatgccttc atcacggtgt atgtgcatgg attccgcaca
     1921 gagcgctcag ctattcaaag ttatgtgagc gatttccctg atttgagaaa ttatttgggg
     1981 aactgagagg ggagttattc tcaacgcttt tcagcggaac ccgttcctaa tcgcagcatc
     2041 tgccagttgc ccgccgggaa gggcataata tcctcatact gaaatgaaaa acccactcgg
     2101 tagcgacccg ccggaagttc gtcgggcgca atgaagccgg catccagcgt agccgacgcg
     2161 ccgggtgtga ggtgaggcag ccggcattgc aggggcacaa aacaccccaa ttttttaccg
     2221 tccatccaca ccgccatgat ttgcaggcgc gctatcttgc tgaaatccag ttcgtaagga
     2281 tatgggttgt acaaatgtag gctcaggcgc acttcctgtc cggccttgag ttcagcgggc
     2341 aaggcatcgg ctacgggttg tactttataa aacgcgtaga aggtatctat ttctgtgtag
     2401 gtaatggtgt cgcggtccag catgaaaaag gccggatcgg gatttgactg cccgcccacc
     2461 cacagaacag ggcgcaggtg tagttgctct tctccgttcc aaaggtcgaa ctggctgcga
     2521 cggcttttgt aagtacccgc cgaaaaggct ggcaactgcc cggtgtaaaa tgcgtacaga
     2581 gaggcccgct gataggaccg gctgaatgct accggacgac cggcggccag cgcttctgtt
     2641 ttttttgtga atgcttcagg gcggaaaaac tgattcggca gcggcgccca ttctcctatg
     2701 agcaaggagc gggctacgag taccaaaggc agtactacga ctgcaacgcg cagcagatag
     2761 cgggttcggc gcgcatcatg gaggctgtgt gcatacaaca tcaacagcat cggcacagac
     2821 gagatgaccg tccattgtgg ttgcacatgc ccccgaaagg tggacaagaa aaagaacagc
     2881 aaaaatcctg caaaattgac tgccagcgcc cgtgtaaaag ggtccttcca attgctatgc
     2941 cgccaggctc gccaggccat tggccacagc aaggggttgt aaaccaaaaa cacattgagc
     3001 aaaaattccg atacatacca ccacttgaac tcctgtgtgc gctctaccaa atgataacgg
     3061 aaggaaggaa aatcgttgat gtattgccac caaaggtgtg gaaaaaacag gactgccccc
     3121 gtcaagccgg ctacatacaa acgcggattc agcagcaggc gcggatgcga caacaccgta
     3181 aaaccgatga gtaatacgcc gtgatacttg ctgtacaaca gccctgcact gagggcgccc
     3241 agcagtaacg catcgctcca gcgattgtaa agaaccaagc gacgataggt gtagaggaac
     3301 aaggctgcaa aaaagagcag cggagaatcg ggggtggcta cgaagccgaa tacctgaaaa
     3361 atgacaatgg aaaaagcgat gccgaaaaac aacagcggac gctgtgggcc aggccaatgt
     3421 tcgcaggtta atcgccacaa cacccacaag gcgcccacct gcgccaaaac ggcaaccata
     3481 cgaactccca actcgccgcc tatccatttg ccggccgaga taaacaatgc aatcatcggc
     3541 gggtgatcaa agtagcccca gttcaactgc gtactgtaca tccagtagta ggcttcgtct
     3601 tcggtgaggg cactnnnnnn nnnnnnnnnn nnnnnnnnnn nncagccaac cgagaaggat
     3661 cagccaggta gttctgttct gatgttcgga gcgtgttcgc atggttgtta tcacagaaat
     3721 gcctgtgaag tgatgcggtc acttcgacat gaccgcatcg ctcgctcgga tgtcgttatt
     3781 caggctcaaa gcccaatacg atgttgaaat cgcccagtcc tttgaggccg cgttcgcccg
     3841 gcaggcgttg cttgtcgaaa ccgatgccat agtcgaaacc gagcaggccg aacatgggca
     3901 ggaatacgcg catacctata cccgccgaac gtttgagatc gaaggggttg aaatcgcgga
     3961 ggttgcgcca agcgttgccg ccctgagcaa aagccagtac atagatggta gaactcggat
     4021 tgagcgaaag cgggtaacgc atctccaagg tgaatttatc gaaaatgggc gcgggcgaag
     4081 tccgttcggc cgcgtcgaag ttggccgaca aatcactcac ctcgtagccg cgcatggaga
     4141 tgatgtccac gccctggaag ccgaattgtt ggttgttgat accgtcgccg cccacctgga
     4201 agcgttcgaa gggtaccgtg ccgactttgc gattatagga gccgaggatg cctattttgg
     4261 cggaagcctt gagtactaat ttaccaacga tgggcgtgta ccattcggca tcgaaacgcc
     4321 atttgtggta ttcgagccaa cgatagcgct cttcggggcc gaggtcggtg taatctttgc
     4381 cgttgagcaa ggaataaggc agggtaagct gtactgccag cgagatgcgc gagccttcgc
     4441 gggggaagat cggatcgtaa acggtagaac gggcaatggt ttggcgaagg ctgaagttgt
     4501 tgaacttgcc gtcggtaatg aggttgccgt tgtcgtcgga gaaagagcca agggcgccgg
     4561 cagaccagtc gttgagactg agcgtttgga ggctcagggc ggtagtggac aggaagttgt
     4621 cgtcgggcca tttgaggcgg gtgccaaggc tggcagaacc gttgaggatg ctgaagctgg
     4681 agttggcggt gaaggaataa cggttgtaaa aaccagctac ggtgaaggag ttgggctttt
     4741 tgccgccgag ccagggatcg gtgagcgaga tgttgtacga ctggtaaaaa ttgccgttgg
     4801 tttgagcacg aatgctgagg cgctggccgt cgccctgtgg cagcggtcgc caggcttctt
     4861 tattgaaaat attacttagc atttctnnnn nnnnnnagaa gttgttgaac gacacgccca
     4921 gcgtaccgat gacgcgctga atgccgcccc agcctgccga gagttccaac tggtcggagg
     4981 gcttttcttc cacggtgtat tctatatcca cagtgccgcg ctgcgggttc acgggggtgt
     5041 tgatccccaa agtttcggga ttgaaatagc cgaggttgac gatctggcgc tgcgagcgga
     5101 ggatgtccga tctgctgaat ttttcgcccg ggcgggtgaa gagttcgcgg cggataacgt
     5161 gctcgtgggt gcggtcgttg cctttgatga tgaccttgtc tatggtggcc tgcgggcctt
     5221 caaaaatgcg catttcgagg tcaatggagt cgccttcgat ggctacttcc acggggtcca
     5281 cgttgaaaaa caggtagccg ttgtccatgt ataaagtgct cacatctcga ccgtcctggc
     5341 tgaagcggag gcgtttttcg aggagttcct gattgtaaac atctcccttt tcgatgccga
     5401 gaatgccctg aagggtgctg ttgtcataaa tggagttgcc cttccatacg atgttgcgga
     5461 agtagtaacg ctgtccttct tcgatgagga ttttgacgcg gaggcggcca tcttcgtctc
     5521 gccagacgct gtcgccgagg atacgggcat cgcggtagcc gatggtgtta taatatttga
     5581 cgagggcttc tttgtcggtt tcgtagtctt tgcgcacgag tttggaagag ccgaagagtt
     5641 tgcctttctc tttggtctcc ttcattttgc gggccaattt tttctctttg gcgttctgag
     5701 cgccggcaaa ttcgatgtct tcaattttga tgcgttcgcc gcgatcagta tcgaaataaa
     5761 cgataacgga attgacacgg gcggtatccg gctcctccat taccttcact ttagcatcaa
     5821 aaaagccctt gtctataaag aatttctcga tggcgcgctg ggcattgagt tttacgtctt
     5881 cggtcacgat gccgcctttg agcatgaact tattgacctg ttcgttgagg ttttcgtggt
     5941 aggttttggg cacgttgcgg taggcgtact ttgagaggcg gggcctttcc tgcaattgaa
     6001 tttccagaaa aaccacatct ccgatgactt tgtccaatac gatctgcaca ttggtaaaca
     6061 aacgcaaacg catcagggct ttcatagcgc ggggtatttc ggggccgggt atgcggattt
     6121 tgtcgcccac tttgaggccc gatacgccga tgatggccac gtcttcgctg taggtggcgc
     6181 cggtcacttt aatgccgccg atttcaaatt ctttaggctc gccgaatgga tacacgggca
     6241 ggttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcagtct
     6361 ggtgtatgga ttcaaagtag ttattcatta gccgtgagct gcgaggttga ttttgcaaca
     6421 aacgcattac gccagacggc gaatcattgg gcgccggttg gaacggatta acgaaagcat
     6481 cagattttaa tttcattggg ctaattgttc gctggtcttc ccgaagcggc gttcgcgttg
     6541 ctgaaagtca atgatggctt ggtacaggtg ttctttcctg aattcgggcc acaacaccgg
     6601 cgtaaaatac aactcggcgt aggcaatttg ccacagcagg aaattgctga tgcgctgttc
     6661 gccgctgctg cgaatgagca gttccgggtc gggaatttga ttcatgccgg tgttcaaacg
     6721 ggcggcaaag cgctcctcac tcacctcttc gggcttcaat tcgccggcct gcacctgttc
     6781 tgccagtagc cgggctgcct gtagtatttc ccgacgggcg ctgtagttga gggccagcac
     6841 cagcgtcatg cgcgagttgt ttttggtagt ttcgatgccc tgcaaaagag cgcggcgggt
     6901 ggcgtcgggc agttgactga ggtcgccgat ggccatcagg cggatgttgt ttttcatcag
     6961 cgttttgacc tctttgtgca gggtttccac caaaagccgc atcagtgcgc ccacttctat
     7021 tttggggcgg ttccagtttt cggtggaaaa ggcgtagagc gtcaggtatt ctatgcccaa
     7081 ttcggcagcg gcctctgtga tgtcgcgcac tgcctgcacg ccattgcgat gcccgaatac
     7141 gcgcggcttg ccatgttgtt tggcccaacg cccgttgccg tccatgatga ctgcgacatg
     7201 gcgcggcaat ttttccagta tgatggctga tttcagttcc attctgacgg cttgcatggc
     7261 cggtttttcg gcggcccggt gcataagcgc gcaaagttag caaaagcggc tttcattgta
     7321 cggattatcg ggcgattcgc tgtacttttg cgcccttgcc acccaaatga gatgatatga
     7381 ggtttgaaca attgcaatac caacgccccg acatggcaca attcagcgcc cgtttttacg
     7441 agttgctgga gcgcttttcc aatgcggaat cctttgattt acaaagtgaa gcatttacaa
     7501 aaatcaatgc cctgcgcgat gaattttctt ccatgttcaa cctctgccac atccggcata
     7561 cggtgaacac gaaggacgag ttttacgcca cggaaaacga ctttttcgat caggagatgc
     7621 ccaacgtaga ggctctgaat ttcaagtttt acgaaaaact gctcgcctcg cctttccggt
     7681 ctgaattgga agctaaatgg ggccgccagc tttttcagat cgccgagttg agccgcaaga
     7741 ccttcagtcc tgatattctg gaggacctcc aaaaagaaaa cgccttagtg agcgcctacg
     7801 tcaagctcaa agcaggcgcc cgcattacct gcctcggcga ggagtacaac ctatcctcca
     7861 tcatcaaact cgaaaactcg cccgatcgcg gagtacgccg cgccgccgct gccgccaaat
     7921 gggctttttt tgatgaacac gccgccgagg tggacgacat cttcgatcag ttagtagaac
     7981 ttcggcatgg catggcccaa aaactgggtt tccacaattt tgtagaaatg gcttatgcgc
     8041 gcatgcttcg cagcgattac gacgcccaaa tggtggccgg attccgcgaa caaatccgac
     8101 agagcatcgt gcccatcgct acccggctct acgaacgcca gcgcgaacgc ctgcaattgg
     8161 agcgcctgca tttctacgat gaagactacc gtttccccga cggcaacccg catccaaagg
     8221 gcgacccgga gtggatcatc agtcaggcgg cacagatgta tgacgagctt tcgcccgata
     8281 ccagtgagtt cttccgtttt atgctcgacg gcgggtttat ggacctgccc gcccgcgacg
     8341 gtaaatcgcc cggcggctac tgcaccttcc tcaactcnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn atagtagccg caacatcggc atcaacgagt actattggcc ctcctacgaa
     8521 gcctgcgaaa ttcattccat gagcatggag ttttttacct ggccctggat gcgcctcttt
     8581 ttcggcgagg ataccgacaa gtaccatttt gcgcacttgt cgggggccat ttatttcctg
     8641 ccttacggcg tggcggtgga cgagtttcag catttagtgt atgaaaatcc ccaatggacg
     8701 cctgccgaac gccgccaagc ctggcgcgac acggagcgca aatatctgcc ctggcgccac
     8761 tacgacaaca atgccttttt ggaaagcggc ggttactggc acaagcaaag ccatattttt
     8821 tccacgccgt tttactacat agactatgtg ctggcacaga tttgcgcctt ccagttctgg
     8881 aaacgcgacc gcgaagatca tacctccgcc tggaaagatt atgtgcaatt gtgccgggcc
     8941 ggcggcagcg gctccttttt ggaaatgacc cgcttggcca atctgcgctc gcccttcgag
     9001 gagggctgcg tggattcggt gtcgggcgtg gtggccgagt ggttggaggg gcattgagcc
     9061 tatactcctc acccttccgc ttccgccttc agtttctctg ccgtatcggc tatttgcagg
     9121 gcctcgatga tctcctctaa gcggccgtcc attacggcgt ccaagttgta gagcgtcagg
     9181 ttgatgcggt ggtcggtgag gcggttttgg gggtagttgt aggtgcggat tttttccgag
     9241 cggtcgccgg agccgacgat ggatttccgg cgggaggcta tttccgagtg tactttttcg
     9301 tattccattt cctggaggcg ctggtagagg cgttgcaggg ctatttcccg gttttggtgc
     9361 tgcgagcgcg cctcggtgct ttcggtgacg acgccgctgg gcaggtgcgt gaatcgcacg
     9421 cccgattcgg ttttgttcac atgctggccg cccgcgccgc tggcccggaa ggtatccact
     9481 ttgaggtcgg ccttgttgat gttgacgtct tccatttcca atttcggcat cacggctacg
     9541 gtagctgccg aggtgtggat gcggccctgc gactcggttt tgggcacccg ctgcacccgg
     9601 tgcgtaccgg attcaaactt cagtttgccg aatacttcgt cgccggtgat ctccagaatg
     9661 agccggtcgt agccgcccac ggtgccttcg tttacgtcca atacctccac tttccagccc
     9721 tgcgcatcga agtagcgctg gtacatgcgg tacaaatcgc cggcgaagag cgaggcttcg
     9781 tcgccgccgg tgccggagcg aatttcaaac aacacgtctt tgtcgtcgtt ggggtctttg
     9841 gggatgagca aaacgcggag gcgttcgtcc acatcggcca ggcgggtctc caactcggcg
     9901 agttcctcct gcgccatggc cctgatttcg gggtcttcgt cgcgcagcat ttccttgacc
     9961 gaggcaatgg cggcgcgggt atcgagttgc tcattaaaag catggacgat gggcttgagg
    10021 tttttgtaat ccttgctcac tttgcggtag cggtccgaat cggcaatgac gccggggtcg
    10081 gccatttgct cttccagata aatgtatcgt tctttgatgg cttccagctt ctcagtcata
    10141 ataaacgtag tgataaaaag agaaaaaaca aggcccttgc ccgtaaacct gcgccctaat
    10201 tcagggcctg tttgaatatg gatgggcaaa ggtagggaat ggcagggaca agcgggggta
    10261 aagatttcct cgttttagag atcgcaaaaa gtggttttga tgaaaactac gttggtggtg
    10321 gagcaagatc agattttcat tcctgccaaa ccgtcgccac gccataaaga gggaaactgg
    10381 ggtcttttgt aatcaccgta atattttcac acaaagcctg tccgatcatc atgcgatcaa
    10441 atgggtctgc atggtgcgac ggtaagaagc atgcgctcaa aatgtgctgg aagttagtgg
    10501 ggagcaactg aaaatcatgc gcggccaaaa aagcaggcaa gtctgtgaat ggacaagaaa
    10561 gatgaagcct gccgaggttg atctttatac ctatttccca aagcgctgca atacttacat
    10621 atttgtcaag atcagagttg gagaggatag caagtgcatt cgctgaaagt tggttgtctc
    10681 cattcaaaaa ccatatcagt gcatggctat caatcaggta cctcatcagt gcatatattc
    10741 tttgaatcct tccaaaggct cgtcaaaatc cggcatcatt tcaaacatgc cctttgcaca
    10801 gccaaaccca ggcgtaatgc cggatatgtc caccttcatg ccgccccatt caacgatttt
    10861 ttcctcccga ttatctccgt tcggcatcat ggaatcccct tcagctatct gaatggtaat
    10921 ctttttctct tgggaggcag gctctttgac tatctgaaaa ttcaaagagt ctatatactc
    10981 cagcagtttt tgcaaagctg cctgattgtc ggtacgaaga attagctcaa acatggtcgg
    11041 ttccttttta tgacaaaact acatttgggg gccaaaagtt gcttccaaat ttatccgaag
    11101 ccttaccagc cctcgccctc actcgctctc cgtctccccg cttttcttcg gttcgtaatg
    11161 ccggttgatg atcggcaaca gcaacaaact cagcaggccg atgagcacgt tggcgccgag
    11221 gttgaccgaa aagaacgaaa tattggccca ggaaaagctg tcgtcgccgc ccacgccata
    11281 gatgcccaat gcctgttgcg ccatgaagtg aaaggtgccc atgcctgccg gagaaggcac
    11341 cacgacgccc caggagccaa acacaaacac ggtgagcgcc gtgcgccagg tgaggtgagc
    11401 tgtgggttca aacgccgcaa aacacaacag cgtcatcaga aaatacatga cccatatatt
    11461 gacggtgtgc accaaaaaca ggctggggcg tttcaaactg cgcacggtat tgatgccctg
    11521 ccagaagcca atggcgatgt ggcggatgcg ctgataaatg cggctggatt ttaactgccg
    11581 gcgcaataaa tataccgcca gaacgaccac cgccagtgtg ccgaaacccc acaatgccag
    11641 attgcccaag cctcccagca aatcggctac gggcacattt ttgctgaaaa agccccaaat
    11701 cggacccgcc tccaccacca aggctatggc cgtgatactg agaatgcaca gcacgtccat
    11761 gatgcgatct accacgacgg cgcccatgat tttttctacc ggaattttct cgtactgcga
    11821 caaggaggcc gcccgaacta cttcgcccat gcggggnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnntatt gatgagccgg ggccgcgagc cgagcggatg gagcagcatg
    11941 ttgagccggg ccgcccgact gacattgctg acgatgaagg ctgccaacac caacgcaatc
    12001 caggcgtaat tgactctgcc gaagtcggca atcacttttc gggccaggct gcaatcttcg
    12061 ggcgcaatgc ctttggcgcc gcattcggcc tggaatgcaa tgttctggcc ccggtaaacc
    12121 cagtacaaaa tgcccaaacc aatgctgaga aacaacagga atttgaagaa ctgaatgatg
    12181 taagtgcgat acatgcgagt gaatgtgata atcccgattt ctatgtcatc gggacaagtt
    12241 gtgctgattg ctcatcgcga taaaactgaa ctcagtgttt gttggtacgc tttgacgccg
    12301 caaaagaacg aaaaaaaatg ccgccgcttt gcaataaacg aagacttaac gcactacaac
    12361 acccctttgt gccgcnnnnn nnnnnnnnnc cccgccacga gtcctcccgc cgcaatgccg
    12421 atttcgagcc attccctttg tattctgtca atgcggctct gaacaaagaa cggattggcg
    12481 cccgccggtg tgcccaagcc cggttttagt tcccagtttt cggggtcatt gttgtccaaa
    12541 tgcggcatca gcagggcgag cgtaaaagcg gaatcagtgg ggggcagttg atactgaatt
    12601 tcatctatca gcgcgccgtc tgcactatac acagcgatgc ggtcttcgcg tttgtgcagg
    12661 ccgaaatcca tgccgcccat cacattgtga gcctgtggga agaccttccg aaagcgggcc
    12721 tcgtcgcggc aaatgatgag atagtctttg ggaccgatga cggcggcggg taaaagtgct
    12781 tcgttgcggg cgtcggccac gatccagccg tccaaccgaa tgcgctcttt ggtgatgttg
    12841 tgcaattcta tccagtcgcc ggcctgcccg ttcaaagggc atatttcatt gataattagc
    12901 ttattggcca atgggtggct atagggttca aacactgcgc gcagcgaaat gccctccttg
    12961 ctcatcggca gcgctatttc ccggctgccg atgccctccg catcgccttc ccaacgcaca
    13021 aaccggtagc ccggcagcgc tatggccttg aaatgcaagg cttgccgctc gaaatacctg
    13081 ccggagaaat cgccttcgga agccttcacc tcaatgtgat cgttgaccag caaagtgccc
    13141 ccgacggaag cagttgcacg gagttcgcgc agcggcccgg tatgaaaacg gttggaaagg
    13201 tgctgaaaaa catgatttgg gcgatacgtc gcaaaatcgc gcatgatctg cacttgccgc
    13261 tcccatttgg cctcgctgag ccgccagcgt tggaggtgcc ggggcatttc gggcagcagg
    13321 gtccggtact tttcctcgat ggccgccagc acgcgctcgg agtgaaaagt ggtgttgaga
    13381 tgatcgcaaa aacgaagggt gaacgcctgc cggaaatcgg ggttttcgag cagcttgcgc
    13441 aaaatgaagg tactccaggg cggattgggc cagtgtgggc catcgggttc ggtgtggaaa
    13501 gcgaggctgt tgttgcgcca ggctttgggg tcgtgcagcc cgaatcccca gtcggtatcg
    13561 tacagaatcc agcgccagcg gccctcgggc gtttgcggtc gccaaaactt gatgttgccg
    13621 cctgcatcct gattgtcgaa ataaatctgg gcaatctggt agttcatgaa gttgtccaca
    13681 tccatttgcg actgcaccca ggcgtagttg gcgggttcgg ccatgttgcg cgattgcaaa
    13741 aacctgagca ggcgcaggta gtgatttttt ttgccgcgcc gcaggttgtc tcggtgttcc
    13801 aggaggtcta tgctgtcttt gtccaccggc tggtgatcgg cgatgaaata gcggttgatt
    13861 ttttcacgga tgttataaat gccccagtat tcgccattga gatagacgtg cgcgggccgg
    13921 taggcttgtt tttccaaatc ccagtcgtcg agcaggccgc tcatcagtgc atcgcgaaaa
    13981 tgggcgcgtc cgaaatcgct gcctgcattc cgcagcacca gaaatttgca acttttcggc
    14041 ccgtcctcac caaaaatcgg gtagtgaaag cgcttctcac cgtagcgttc gcgggctgcc
    14101 agggtcaatg atttttgtgg aaacaagcgg ctcatgcctc caaaaagcct catacccatc
    14161 tggctgttga acacgctgct gccgtcggtt tcaaaaaatt ctacatgcgc caccaattcc
    14221 cgcctgctcc agaaattggc gccttcctgc atgatgctgc ccgtatccaa gcggttgccc
    14281 gtcataaaca agccgatgtc ggggtcgaac aacaaacccg gcggcacagc taccgacact
    14341 accggcaggc tgctcttcgg ctcgccgatg aagtaagcct gagcatcagg acgactcgcc
    14401 tctccgttgg gataaacggc tacggcgcga acaacagcac tttttttcag caggaaagga
    14461 cgtgtgtaac ggtgggcttg agcattgggc ttgttgccgt ccaacgtgta ataaatgcgg
    14521 gcgccctccg atgcggaaag ccctacggta acgggtgttt cataaaagcc cgacaacagt
    14581 gcaaagtcaa ccccggcaat ggatgcctgc ccccacagca cgctcccgat actgcacagg
    14641 agcaccgaca gacatagttt cagcgacttc cattttgata ccatgttcga gagatgattg
    14701 caagaacggt ggagtacaag ctactggcca caagccgcga gcctcaagct gcaagcannn
    14761 nnnnnnnnnn nnnnnccagt agccaacagc cggtcacaaa ctgtaacttc acttcttacc
    14821 tgccgccgcc ttgatctttt cttccatgtc aaactcattg ccgggaatgt agcccgtgtg
    14881 ctcgtaaatc acgttgcctt tggcgtcgag gaggaaggtt tgcgggatgc tctggaaatt
    14941 gagcgccgcc ggcatagcgc cgttggggtc ggacaggatg atgtactccc agcctttgga
    15001 ttccaccatc gggcctactt ttgccaatgc gcgttgcgtg tcaatggtga cggccacgag
    15061 ttgtacgccg tatttctcct gccattcggg gtacacttcc gagatggcat ccaactcctg
    15121 ctggcagggc ttgcaccagg tggcccaaaa gctgagcacg gtgattttac cgcttnnnnn
    15181 nnnnnnnnnn nnnnnccgct ttgggtgtat ttctggatgt ttacagtttt gccgtccaat
    15241 gttttgatct ccaccgaggg gatggatttt tgggcggaaa tggccgtgac ggaggcgaga
    15301 aggcagaaaa cgaaggtaaa aagttggatt ctgttcatct tttgacaaat ttttgaatgt
    15361 gtagaaagta cctttgcgac aaaaggtaac gcaaagttag gtaaatagca tgttgcgcta
    15421 ctgctaatgt tctgttaaac acgaattatg aaacgcttag gtcatctgct ctgcacactc
    15481 atttgcgcct tcgtggccat tgctcctctt gctgcccaag acaacaacag cggcggtaaa
    15541 atttcgggaa gcctccaact caatgccaat ttcttcatgc gcgattccct catcggggcc
    15601 gccaacacgc cgcaatacga ccgccaatta tacggcggcg aatcctggct caacctcgca
    15661 tacagcaact ggggcttcga cgtgggcctg cgtttcgatc tgttcaacaa ttccaacctc
    15721 ctcaatccta ccggctctta cagcggcgaa ggtatcggac ggtggtacat tcagaaaaaa
    15781 attgacaagc tcggcatcta tggcggctac atctacgacc agatcggcag cggcatcatt
    15841 ttcagagcct ttgaagagcg accactcttg attgacaacg ccttgttcgg cctccgcctt
    15901 agctacgacc tgcatcccaa ctggaaaatc aaagccttca ccggacggca aaaacagcag
    15961 ttcagcactt acaatgccac catccggggc atcagtatag atggattcat tgcggccaaa
    16021 gattccactt cctcgttttc gctggcgccg ggcttcgggg cagtgggtcg caccctcgac
    16081 gacgaatcca tgaactcttt ggtggccatt ctcaatacct atcctcaaga cgacgcattc
    16141 acacccaaat acaattcctt tgcattttcg gccttcaaca cccttnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnaccgggg ataaatttat ttttaaagaa ggcaccgtgt
    16321 tgtactccag tttcagctat gccgccaaag gtctgggcat ttcgctggag ggcaaacgca
    16381 ccgaaaattt cagcttccgc acgcgcccgc aggaacagct caatcgcggc ttggtcaact
    16441 tcctgccgcc catggcccgc gtgaacacct accgcctcaa tgcccgctac aacgccgcca
    16501 cacaggaatt gggcgaactg gccgtacaag ccgacatccg ctatgcgccc agcaaaaaaa
    16561 tagccttcaa cctcaatgcc tccaacatca ccgacctgaa cgacgtgctt ctgtaccggg
    16621 agtttttctt cgacgtcacg ttcaaaaaag accgcatctg gacactgacc acgggcgttc
    16681 agcaccagaa ctacaatcag gagcgcttcg aattcaagcc gggcgtaccc aacgtgcaaa
    16741 ccatcacgcc ctttgccgat tttctgtaca aattcnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnngg aaacgcaata catgcacacc ggaatggacg aggcggcagg gtacaaacaa
    16861 gactacggcg actggctctt cggattggta gaactcaact tggcgccgcg ctggagcttc
    16921 gtcgtttcag atatgtacaa tgccgggccg ggcaaaaact cgcccgccga tgaaaacggc
    16981 aagaagctca aactgcacta cccgcgcttc gatgtatttt acacactgaa gtccaatcgc
    17041 atctcgctca gctatattaa gcaggtagag ggcgtggttt gcaccggcgg catctgccgt
    17101 ttggagccgg cgttcagtgg ggtgcgcctt tccgtcagtt caaccttctg atcctgcgct
    17161 tttgcaattt tatcatgaat gcgctctatt ggtaatcagc acatcagcac attgttctat
    17221 cagcacatca agatatgttt ctcgcccaac tcctctacta catcggcgcc ctcctgagcg
    17281 tgcccttgct gcctgtttta tattttcaag gcaaaaagct gcgcaaaaac ttcccgcgcc
    17341 tgcccgaacc ggaacacaat gtgcagggcc gcacagagga aggcgttggc gcgcccattc
    17401 gcatattggc gctgggcgag tccaccattg ccggcgtggg cgttcaggac caccggcatg
    17461 ggtttacggg agagttggcc gcggcacttt ctgcacacac aggccgtccg gcagagtggc
    17521 aggtagtggc ccgcagcggt tatgatgcca aagatgtctt tcggaaattg actcccaaaa
    17581 ttcctgatgc gccctatgac ctgatcgtca tcggcctggg cggcaacgac actttttacc
    17641 tcaacagccc gctgcatttt cggcacaaca tggcccactt ggtgcgccat ctccgcgaac
    17701 gcatgcccga tacacccatc gtcatagctg ccctgccgcc gataggagtg tttccggcct
    17761 tgcccggctt ggtgcggtcc gcactgggcg ccttggtaca cctgcacggt tttgccattc
    17821 gcaacattcc ccgccggttt gccaatgttc attaccccgg aggccccatt cgggcgcgcc
    17881 gttggatgga cgacattcac tcgccggagc agtttttcag cgatgnnnnn nnnnnccgtc
    17941 cgccgaagcg tatcgcctct gggccgtaga aaccatgcgg tttatagcag ggtgcggatt
    18001 gaaatggtaa atcctaaaaa ttgattgatc gctgctcatg aggtatcaac tcgctgccat
    18061 cggactcatc atcggtatct tgctgccctc agcaagtgcc gtgctgatgt accgggttgc
    18121 ccgcagccag atcaggagcc gcatagaaga acaaattgca gcgggcaaag cagaagacgg
    18181 tttggttttg ctgcatattc cccgccggtt ggaagaccag cctaatgctg atttcaggcg
    18241 atttcatgct acggaattca ggtacaaagg catcatgtac gatgttgttc gtcaggaggc
    18301 aaaaggagat acaaccttgt actggtgcat tgcagacata gaagatacgc agctccaaaa
    18361 tcaatgggat aactactggg cacgaggttc ccggtccgac aaccctgctc aaagagctgc
    18421 cgaagaccga ctgtttcaat tttggcagag cttgtttgtg cattcattgc cgcctgtaaa
    18481 ccatctgttt gggagcggcg catcgtcaaa accccggcca tgcgccaaca agtacagctc
    18541 tgtttcacgc cccattcccc agccgccacc ggaacctctt ttcccggttt tggtacaatg
    18601 ctgatttttg ttcgctcgga ttttcctgtt ttgtggtaat aatgatacgg cacaaatcag
    18661 agcgagcatt gccattgtac atctgaggta tgccgttggc gtcaagtgtc gtcaatggcc
    18721 tgagttgttt atttaaaaca ctaataatga gaaatttata tttcatcttg ctatgtttgg
    18781 tctgcatggc aagtacttct gtatatgccc agacgctcat cgttcaacag gccggcaccc
    18841 gccaggcctt agagggcgtt accattacct cggcttccgc caatgcagtg accgatgccg
    18901 ccggcaaagc cgatatttca acaatgaaat cggataccgg cgaaatacaa atacgctcgg
    18961 tgggatttga aaccctccgt acccggtatg ctcttttgga aaaacaggga ttcatggcca
    19021 tcatgcagga agaccgcatt tcattggatg cagtggtggt ttcggccacg cgctggcagc
    19081 agtcgcgccg cgaaacaccc ttgagaatca gcaccatcgg agccaaagaa gtgatgctgc
    19141 aaaacccgca aacggctgcc gatctcttgt cgctgtcggg agaagtattt attcaaaaaa
    19201 gtcaattggg cggcggcagt cccatgattc gcggttttgc caccaatcgt gtgctgctca
    19261 cggtgtacgg cgtgcgcatg aacaacgcca tttttcggag cggcaacctg caaaacgtca
    19321 tttcgctcga tcccttttcc atccagcgca ccgaggtggt gtacgggccg gggtcggtca
    19381 tttacggcag cgatgccatc ggcggcgtga tgcacttttc caccttagaa ccggcgtttt
    19441 cctctgtgaa tgccaacgcg gctgttcggt acgcttcggc caacaacgag aagaccggcc
    19501 atgcagattt ttcggtcgga ggcaaaaaat tggctttcgc cggaagcgcc accttcagcg
    19561 attacgacaa cctgttgatg ggcaaaaacg gccccgacga ctacctccgg ccccgctatg
    19621 tgattcgcat caacggccgg gattcggtgg tggccaattc caacccgcgg cttcaggtgc
    19681 ccagcgctta caaccagttc aactggatgc acaaactccg gtgggcgccc ggcgaaaaat
    19741 gggaactgca atatgggttt cattactcgg ctacatccga ttactcgcgt tacgaccgcc
    19801 tgcttcggcc ccgaggcaac acgctgcgca gcgccgagtg ggactacggc ccgcagattt
    19861 ggatgatgaa caacctgtcg gcggcgcatc attccgaaag caaatggttt gataatgtgt
    19921 cgtttagagt ggcgcatcag ttttttgaag aaagccgcat agagcgcaat ctcaacagcc
    19981 tcacccgaat gattcgcacc gaaaaagtgc atgccctttc ggccaatgtg gattttgaaa
    20041 agacacgcca aaacgggcag cgcttttttt acggattgga ggccgtttac aacaaggtcg
    20101 gctctaccgg cgaggatgta aacatcgaaa cagagctgcg cagagcggcg ccctcccgct
    20161 atcccgacgg cgccctgtgg tattccgcag cagcctacct cacctggaag gccccgctga
    20221 gcgagcaact gaccttgcaa accggcgccc gctacaacat cgcaggcttg caggcggagt
    20281 ttgatactac cttgtttgta ctgccttttg cagaagcccg gcaggcatcg ggcgcgctga
    20341 caggaagcgt cggggtcatc tggtcgccga cggcgctgtg gttgttaagt gccaacgcgg
    20401 cctccggatt ccgcacgccc aatgtggacg atgtgggcaa ggtgttcgat tcttcgcccg
    20461 gctttgtggt ggtacccaat gcagacatca ggccggagta cgcttacaat ttcgatctgg
    20521 gtattgcgcg aactttcggg agtacattga aggtagatgt gacgggcttt tacaccttat
    20581 tagacaatgc tttggtgcgc cggaatttca cccttaatgg ccaagacagc gtgttgtatg
    20641 gcggcgaaat gagccgggtg caagccattc aaaatgcggc tgtcgccaaa gtctggggac
    20701 tgcaattggg tttggaactc aagctgccgg cgggtttcgg cctccgagtg caccacaact
    20761 tccagaaagg cgaggaggaa ctcgacaacg gctccgttgc gcccctgcgc catgcagcgc
    20821 cctcgtttgg aactacccgg ctcacctgga gcaaccgcaa atggcaggcc gaattgaacc
    20881 tgatatacaa tgcggaagta gccaacgcgg atttagcgcc cgaagaacag ggcaaagaat
    20941 acctctacgc ccaagacgcc gccggaaatc cctacacgcc ggcctggcac acgatcaacc
    21001 tgaaaacttc gtatcaattg ggcaacaggg ccaatatcgg cttcggtatt gaaaacatca
    21061 ccgaccagcg ctaccggcct tattcctccg gcatcaccgc gccgggcagg aattttgtgg
    21121 tctcgctgag ggtgaaggca aggtaggaac atcagcctga caggaggggc gcttcgtcgg
    21181 aaaaaacaat aaaaatttcg ctggtagcgg gggtgctata gagcgtacct gctaccactt
    21241 ttttgttttc tcctgaaaac acgatagagg aaacgcccgg catagagcta acaggtctat
    21301 ccggctattg cgctgcagac ctgttagctc tgcaatgaga agcctacaac ttggagccgg
    21361 ttgcgttgtt ttttgcatcc gatctgcatc atacaggtgc gccgcctgaa accggactgt
    21421 cggagaaact caaggtgttg gagtaagcca cctcccgcct gcttcgctat acctacgcca
    21481 gtgctaacct gccgaatatt gaactaatcc acaggcgatg atgtcagatt aaaatcatgc
    21541 tgttcttgtc aatgatgaca ctcacagctt gcattgatag tagaaagaat gctcctgcgg
    21601 aaaagaattc gctgcaacat caaacccgga tactcaatac cggcaactgc ccatagcgca
    21661 acaggttttc ggggatgctc tctacgaaga tgccgcccaa gccggtgcga ccgtgattgg
    21721 caatgccgat gaggtcggcg ccgacggact gcgcaaactc cagaataccc gcctcgatgg
    21781 tgtagtgtgc gtagctgtgc aggctgatgt gccggaaatc gtgcgcatcc acaaagtcgc
    21841 ggattttgcc cattacttcg cccgtaggcc ggaaattgta cggcgtggtg acctctacaa
    21901 aatagagatt ttctgcgttc atgctgtcgg caaaacggag caactgatca aatgcggggt
    21961 gcgtatctgc ttcgaggccg gaggcgaaga caatggtatt gaatttgagt tcgtcacccg
    22021 gttttttgca ggccaatacc ggcgcctggg cagtacgcat gattttttca gtatgagagc
    22081 ctaatgcaaa ttttttgacc cccgtcagcc catgcgatcc catgacaatg aggtcgtttt
    22141 gagtatcaag caccgtgttg gcaatggttt cgaagccttc cagaaaagcc agactttttt
    22201 cagctttgag accttcatgt tcggtggcaa gcaccagctc atccaacttt tggtgggccg
    22261 ctccaatctg agccttgatt tcgggatatg tgtcttcgag gcgcttgggc aggttcaccc
    22321 agtcaaccgg cgtttttaag ccgtgcagat acaggatttc cgcattgact tttttggcga
    22381 tccatctccc gattcgagca gcggtatcgg cgcacggaga aaaatcagtg ggaatgagga
    22441 tctttttcat attgtaaatt ttgaggcgtt attcaatttt gatcttccga taagcgcgct
    22501 catcttccac cggcgtgtgg gtgaaactca gcttgagaat gccgtgctcg caggaggcgg
    22561 caatttgttc gtgagcaatg cccgacgcca gtttgaaagc gcgctcaaac gagtcgaagt
    22621 cgaattcctc cataataaat tctgctcccg gacggcgctc ggcgcgactc ttttcgcctt
    22681 tcacaacgag tacatcgtct ttcagggtga tctcaatttc ctctttggta aatcccggaa
    22741 cggccaactc caactcgaac aaatcgccgt cttttcggag attggcaggc ggcgtactct
    22801 gttggtatgg aatatcgaag gaactgcgac cgagaaaatg attagaatct accactggat
    22861 gaaactcttc gccgaagcgt tcaaaagcgt ggtttttgat ctctgctctc cacatggaac
    22921 gtacgttttg tttgtacgcg taatatacag atatggagcg cgtcggcggg gtgcggtttg
    22981 tcagtgaggc ggggtgattt ttatcaatag aagcagtgtg ccgcccaagc tacaactcct
    23041 ttcgcaggcg cgccacggga atgttcagtt gctcgcggta cttggccaca gtgcggcggg
    23101 ctatgttgta gcctttttcg gtgagcagtt cggtgagttt ttcgtccgaa aggggtttgc
    23161 gtttgctttc ggcagagatg aggtcggaga ggattttttt aacctccagt gtagaaacct
    23221 cctcgccttc gctcgtttgc agcgactctg aaaagaagtc tttcaggcgg cgcgtgccga
    23281 attccgtttg cacaaacttg ctgttcacta ctctggaaat ggtcgaaatg tccaatccgg
    23341 taatatcggc tacgtccttc aggatcatgg ggcgcaattt gcggtcgtcg cccgtcagga
    23401 agtagtcgta ttgatactgc atcatggcgt acatggtgcg atacatggtg tcctgccgct
    23461 gtcggatggc atctatgaac cacttggcgg agtcaatttt ttgccggata aaaaggatgg
    23521 cctccttctc gcgcacattg gggcggcggc cctgagcggc ttttttgtaa tcgcgcagca
    23581 tgtctttgta gtgatcgctg atgcgcagat cgggagcatt gcgcgcattg agcgtcagtt
    23641 ccaattcgcc gtcgttgttc gagatgatat aatcgggtac cacatattgt gtggatgacg
    23701 acgaccccac gaacgcgccc gccggcttag gattgaggcg cagtatttcg tctatggctt
    23761 ccttgagttc ttcttccgac acatcaagtg cgcgctgcaa cttgtcgaaa tgctttttgg
    23821 taaactcgtc aaagtggcgg ctcagaatgc gcgctgccaa ctgaatgggg cggcggtctt
    23881 cctccttcag atgttcctgc tccagcagcc attccaattg caataggagg cattcctgca
    23941 aatcgcgagc gccgatgccg ggcggatcga agcgttgtat ctttttcagg atggccttga
    24001 tttcggcctc tgtagcgctg atgttttggg aaaaaagcag atcgtccata atggaatcaa
    24061 tctcgcggcg caggtagccg tcgtcgtcaa tgctgccgat gatctgccgg gcaatggctt
    24121 cctgcgtggg gccgcccagt tccatcatgc ccacctgctg ctccagatag tcgtggaaag
    24181 tactttcaat ggctaccggc agcgtgcgtt gctcttcatc ggcgttgtaa gcatctccgc
    24241 gcgttttgta agaagcctcg tcgtctgcga tgtattcatt gagatagtcg ctgaattcca
    24301 gtgggttatc tttttctttt tcaaaatcgg gcgacacttc gtccccctcc cggtcgtcgc
    24361 tgctgcgctc ttcgtagccg ccgtcctcat cgtacatttc gtcctcggta ctgtcgtcgg
    24421 aatcttcgct gtaatcgcct tcttccaatg cggggttttc ctccaattct tctttgatgc
    24481 gctgttccaa aatagcagta ggcacctgca acagtttcat gagctgaatc tgctgcggcg
    24541 ccagcttttg gagcattttc tgacttaaac tctgcttcaa catgtttttt gtggttgcgt
    24601 cccatgtcgt tccagacggc aacgggcggc taaattactg ctttttccaa tgccgggcaa
    24661 gctgacggtg cgtacctgaa tgcagtttaa caaaaaatat gccatttttt tataatgtgt
    24721 ttccaaaaag cggccatgcc ggcaaaatca actcgtgtaa ggggagtgca gacagccccc
    24781 tgttgcaaga ggcgaaataa aatttctatc catacttgtt gcaaagctga aaatgtttct
    24841 atctttgcgc gcctatttcg aggcataaag tataaagccg taataatcat ggaaataatt
    24901 gcaataaatg gtcagcccag aacggatttg ggcaagaaag gcgccaaggc tgtaagaaac
    24961 ggagagctca ttccttgcgt gctgtatggc gcaggcgtat cggagcattt ttctaccgaa
    25021 gtgagcagcg ttcgccacct gatatacacg cccgatttca aagtggccga aatcacggta
    25081 gagggcaaga agcaccgctg cattgttaaa gccgtgcaat accacccggt atcggaaaaa
    25141 atcatgcaca ttgacttttt gagccttgtg ccgggccacc cggtcaaagt ggaagttccc
    25201 ctgcgtttca cgggttcttc tatcggtgtg aaaggcggcg gcaaactgca acaaaatgta
    25261 cgccgtgtaa aaatcaaaac gatgccggaa gacctcgttt cggaattgaa attggacatt
    25321 tccaatctgg atatgggcca gtctgtccgc gttcgggaca tcaaagctcc tcagaacgtg
    25381 gaaattctga ctgctgctag cacgccggtt gccaccatcg aaattccgcg cgcattgcgc
    25441 agtgcggccg cggctgccga taagcctggc aagaagtaaa aaataacatt ttctttatat
    25501 cggcctttgg tcgcacttcg ttttcaggcg gtgtgtggcc aaaggccgtt ttattttaac
    25561 ccaatatcat tcactcaaac actacattat gttgcgtacc ttctcattcc tgctgttgct
    25621 cacgctgctg agttcctgca cagcttccga acttcagcaa ttactgggca gcggcgaact
    25681 cccactcacc caggccgaag cggcttcggg gcttaaagaa gcgctttcta tcggcatcgg
    25741 caaaggtgcc gaattgctgt ctcaaaaaga cggttatttc aaaagcgaat acaaaatcct
    25801 tttgcccgcc gaggcccgca aagtcacaga aaaactcaaa gtagtgccgg gcttttccaa
    25861 tgtggaagaa atcattctgg aacgcatcaa ccggggcgcc gaagatgccg ccatgaaagc
    25921 caagcccatt ttcctgaacg ccatcaagga gatgagcttc acggatgcca tgaacatcct
    25981 tttgggcggc caggatgctg ccacccgcta tttggaatct gccaccaacg aacaactcta
    26041 ccagcagttc aatcctgtca ttatagaatc gttggatcgc ctcaatgccc gcaaatactg
    26101 gtcggatgcg gtgatggcct acaacaagat accctttgtg gaccgagcca atccagactt
    26161 agacgactat gtgacccgcg aagccctcaa aggcatgtac ggcatggtgg ccaaagagga
    26221 gctgaacatt cgcaccaatc tgagcgcccg cagcagcgac ctgctcaaaa aggtgtttgc
    26281 caaacaggat aaaaagtaac caaggacgaa ggctggatca caatctccgg cctttgttgt
    26341 ttttccccca acccatttga cgacaatatg cttgccgtat aaagcccttc ccttttcgtc
    26401 gagtaatttt cgactttaca ccaacagaaa cacagttttg agtgtctatc ttttctcaat
    26461 aacaataacc aaagaccatg tacattcgac acctcctatt gacggcatca cttctgtgta
    26521 tttccctttc cctttcggct tcgggcatag ccgggcaagt taccggcaac tccggcgaag
    26581 cgcttccgtt tgcagccatt tttgtaaaag aaaccggcag cggcacatcc gccaacgagg
    26641 acggctattt cgagctgcga ttggagccgg gcagatacac ccttgttttt caattcttag
    26701 gctacgaaac cgtttcccgt acggtggacg tccgcagcgg cccgttcacg atggtcaatg
    26761 tggcgctcaa acagcaggcg gtacagcttc gcaccgtcga ggtatcgagc aaacaggaag
    26821 accccgccta cacggtgatg cgcaaggcca ttgccaaagc cggctaccac aggcagcaaa
    26881 tagagcgtta cacggcgcag gtgtatatca aggggtcggg gcgactcaaa aaagcgccct
    26941 ttttcctgag aaaggaattg aaaaaagaag gcatagattc ttcccgcgct tttgtgagcg
    27001 agtcggtgag ccaaatttcc taccagcggc cctctacttt taaagaaaaa gtcatcagca
    27061 tccgcaaaca gggcaacgac aactccacca gcccggcgcc cttcatcaac ggcagttttt
    27121 acgagccgga aattgccgaa gtcatctcgc cgctttcgcc caaagccttt gcgtattaca
    27181 aattcaaaca tgccggttac ttcatggacc ggggttacgg cgtcaataaa atacaggtca
    27241 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnta tagcctcgac ctgaaaacct acaaactcgg catcggcatt
    27361 gacgtagctc aaacctacgc ccctattgaa gacaaagcct ggctgcccat caagcatcag
    27421 atagaagtgg acggcacttt tttcggattt cagtttgagt acaaatacat cgccacagtg
    27481 agtggttaca aaattgaact gaacccggcc ttagacgtgg atttcaaagt gttggatgaa
    27541 aaaatagccg ctccggaact cgtacaaacc gccaaaaaac aacagactgt cgccaaaaaa
    27601 tcggctgccg aacaacgctt ggcaaccggc gaagaactga ccatgaaaga cctgcggaaa
    27661 atagtacgcg actacgaaaa ggaagatcgc aaaaaagccg taactgaaga caaagactct
    27721 ctggccggct ttgcttcggt caccgaatac aaaatagact ctctggccga gcagcgcgat
    27781 tctgcctact gggccgaaat ccgcccggtg ccgctcaccc tgtacgaggt gcgcggctac
    27841 cagcatgtgg acagcattgc cgttgtggag gctgacgaaa agaccgagcg cgactccttg
    27901 ggcatcgctc ccggcgaaga tgaggaggta gaaaaaaaac gcgacaaatt caagccgcag
    27961 gagttggttt tgggcggcag ttaccgcctc ggcaagcgca gccggctcag cgtcggctcg
    28021 gtgttggcca atctcaattt caaccccgtg gagagtttcc actcctggag cgacctnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28261 nnaacccgat cagttcaata atgaacacag cttacaacct gttgcaggag cgcaactaca
    28321 tcagactcta tgaaaaggaa tacttcaagg tgcaggtaaa ccaccaattg cgcgaaaaca
    28381 tcagttttca ggccggaggc gaatgggcgc ggcgcttctt ccccgacaac cgcaccacac
    28441 agacgtggtt caaacggcgg gagggaggtt acgaatccaa tatcccggtg aatgaagaat
    28501 gggccggtcc gcccgctgag ccggagaaag ccttcgtact gcacgccact gtggaagctc
    28561 gtccctggca gaagtaccgc atcagaaacg gcaaccggca ggccatagaa gactcttcgc
    28621 ccaccattgg attgtcttac cgcaaaggca ttccgggcat agggggcagc gtgacggatt
    28681 tcgaccacat tgacctcagc ttccggcaca gtttccggcc cggcgcccgc ggcaaaattg
    28741 acatcaaggc agaagtgggc gcttttttga acaacaacta cgtcggattt gccgattacc
    28801 gccacttcat gggcaatcgc attccgattg tcactaccga ccctgtgggc agcttccgct
    28861 tgctcgacta ctatcgccac agcacctccg aggagtacgc ggccttgcac ctgcattacc
    28921 agttcagaaa actgctgttg acgcaaattc ccaaagtgtg gctcaccggc gtgaaagaaa
    28981 acctgtttgt caactactta gctacgccta cctcgaagca ctacaccgaa ttgggctact
    29041 ctttagacaa tatcctccgg attttcagag tcgaaacggc agttgctttt caaaacgggc
    29101 gctactacga ctggggcatc cgtatcggcg tggcgtccaa tctgggattt atacgatttg
    29161 actgacggtt tactccaaaa tatctgaacg ccatgaaaga ggatagccaa attgcgatat
    29221 accaaactga cgacgaacgg gtaagtattg aagttcaagt cggacaggac agtgtgtggc
    29281 tgacgtaggc acagatggct gcgctgtttg acagggatta caaaactatt tccaagcnnn
    29341 nnnnnnnntt ctcaatgctt tcagagaagg agagttggaa aggagtgcag ttgtcgcaaa
    29401 atttgcgaca actgcctctg acggcaaaac ctaccaagta gaacactaca accttgacgt
    29461 catcatctcc gtaggttatc gtgtgaagtc gaagcggggt acgcaattcc gcatctgggc
    29521 tacgcaggtg ctgcgcaagt acctcataga gggctacgca ctgaacgaac agcgactgcg
    29581 ggaacaggag caaaatctgg aaaacctaca ggaagccgtt catctggtag cgcaagtggt
    29641 ggtgaatttg atcaacgcga aaaattgatt atgaccaacg aaacccataa aggcactgtc
    29701 atccagaggg caccctcagt gtgaacggcg aggcgcagga aatccgcaat gaacagacca
    29761 ccctgcgcac cctgccggcg cgagcgggga aatgaaatgc gtctgacttc caaaactttt
    29821 acattcatgg caaaaagcaa gctcgctgaa ctgagtggcg caaacgaggc tttttatcaa
    29881 atccaaaaaa ttattttcgg caagacattg attaagccat gagtcaaagt tgacatcaga
    29941 atgctgccgc ttttgatagt catccacccc aacaacaacc cgattgcaaa tgtattaaag
    30001 aactcaaacc aattttgatg aacagtccaa tcgttgtcta tttgaagaga gtgccctaat
    30061 ccaaatagaa tggaagttat caataaagca ggattcccta aataaacaga gcctattttt
    30121 atttttgact ttaaagaatt ggataaaagc cccatcatga ttcctctgaa tgccagttct
    30181 tcgtcaatac cgggcattgt aaattggaac agaaaatatt ctaacttttc atctgatttt
    30241 tttacgcata aaaaagccag gccaatggcc agaaggaaaa caaaaatggt aataaataat
    30301 gcaggtttta aagatttttt tcgctgttta aaagtgatat aatcaatatc tgtaaatgtt
    30361 tttttgaaaa gccaataaaa caatagcgaa cctgcgattg ccaatatttt ccctgaccaa
    30421 ttccagttat cactctgaat gatttttgtt tctggaaaca aaaaaggaag cgataaaaag
    30481 ctcgcataga aaaagtagta aattacaaac aatataagtg tcttccattt gtagtcaggc
    30541 ttatttaagc ccaaagctac aagtggcgca atcaccatca attgtataga caaaaaaata
    30601 attaattttg ccatgctttg tcatttttgg atgtcatttc ttcctttccg ataaccccca
    30661 cggccttttt gcgaagagag cagcgtcgtt atgtatagaa gaaaaagctc tcgtaatgcc
    30721 ctcgtcccct tgtatgtgaa agctattcca cttcggcctt tactctctcc acccggcagg
    30781 cagatgcacc tctccggtgg gttccgccca tccctgatag ccgttgggag tgcttaccat
    30841 cagccgatca cccttgacct gaatttgaga gaactggggc ggcagggttt gacgcccatc
    30901 ctgaccggca actcccaccc ggtcgccgag atacagcagt gcgacgggag gatggtaatt
    30961 ccctttgtgc cacgagacgc ggtcgtaaat aggttcggtg atgaacgctc ccacttcgtt
    31021 gacaaagccg aacttaccgc tccgcgcgat cataatatgt actattttgc cgaagtcttg
    31081 ttctacatgc ttcacctcgc agccttgcgg aataacgaca tgcttcacga tcttccaggt
    31141 tgagtggtcg gtgctgcttt ccagcatgac gaccgacgga aggttctcca aatccgccac
    31201 tcgtgtgcga tattctactt tgcgcttcgg ccaagactcc tcaatgtctt catctgtaat
    31261 atcctgagcg aaatagacag cctcttcttc cgccatcggc gtgccgttgt cggcaatgcg
    31321 tgtgattttt cctttgttgg taatatggac gtacagccct tgttcgaagt ctgaatagat
    31381 gctgacttct tcattcacgc agggcaccag aagtttgccg gtagtctgga ggacgcccca
    31441 gccgctgccc tgacgcactc ggaccaaagg ctttttgcct tgtgcgaaga agtccacctc
    31501 atcaaatatg ggtttggtca gccacttggc ttttttgccg tccacatgga gcaggccata
    31561 ttttccgccc aaaactgccc agcgcaagcg aggttgagcc aaccacatga tactgtcaac
    31621 acttggcttc agcacccatg cccccgcagg agttatcaga cctgattttc ctcccttgcg
    31681 agccaccgcc aagtgcatgc tttcgccatt atcgagtgaa aacaagccaa tggagtctaa
    31741 ggcagggggc atcgtcactt ggccgttttt gtccacaatg ccccatttgc cctgatgagc
    31801 atacggaaaa acttgctgcg cctgaactgg ctttagcgcc cacaaagtca tacaaagtgt
    31861 aagaatagtt cgtgtcatgt tggttgagtt gtgttggttt tatgatactt taaagccacg
    31921 accgttgcct ccaaccgtct ggcctatttc attcgcatca atcccaaatc ccttcctctt
    31981 caatctcgaa ctccgcgtcc ttttcccaga tttccatttc gcagggcttg cagttgaatt
    32041 tgagtttgta ctccacttcg ccgtgtttca gcaccaaggg ttcttttact ttttcgccca
    32101 tcgtttcgcg ctggaaccgg gggcatttgc ccaactcgta tttcacgcag tatttggtcg
    32161 tcatcacgcg cgacttgccg gggtcccatt gcagttcaaa tgccttttcg atggactgca
    32221 cgccatgacg ccggtaaaac tgccgcgcca acttgttcga cacattgtag gtgaagtcca
    32281 attccgtcac agggtagggg tgctcggtag gcgctatggg gcgctcctct ctatgatatt
    32341 cccggacacg ggtatccatc aattgctcca gcaccgtgcg gcgaacctcg ttgattttgg
    32401 aaatgggaag gaaccaattg tctgaaaaat ccacctgaat atcgtccacg atgaaggggg
    32461 tattgcctgc tttggctaag ttttttcgga tgttgtcaat gatggactcg ccgtttttgg
    32521 tcctttcttt tgctaccgtc agggtggcgg tgctcgtatg tccgtcctcg tcggtggccg
    32581 tgagcgtgaa gccttccaga gactcagcaa agtgaagtac taccccgatt ttgcgaacgg
    32641 cgctgtcttc gcgttccagc aacttgttga attcggcgtc gaaattccga tagatgtctg
    32701 ttccggccgg cagcggtcgg aagttgttgg gtaccaccaa accgttgacg atgatattca
    32761 cgcgatcgcc gtcggcccgg ccttcttcgt tgacaaaata gaggccgtcg ccgttgttca
    32821 gcagttcggc attttcgatg acgtatccgt tggctttggt ttccaatagt ttaccaatgt
    32881 attcgccttg cgatttgggc gtctcccagg aaccgatgcg ttctttgcgg tggttgacga
    32941 aatagtcggt atagccccga ttgaagctct tgtccatttt ggcttcaaag ccgtaaaacg
    33001 tgcggccgga cgaagccttt tcgtagtgcg gattgtcttc cagaaaggca tccaatcgct
    33061 tgcggaggta agacacgttg tttttgacat agaccaaatc cttgagccgt ccctctattt
    33121 tgaacgagcg gatgcccgcc tcaatgagca cgggcagttg gtcgctgagg ttcaggtctt
    33181 tgatagacag caggtgggtt tccgtgagca gggtagtacc gttgccgtct atgaggttgt
    33241 agggcaggcg gcagttttgg gcgcaagacc cgcgattggc gctgcgctcg ccgttggcca
    33301 cgctcatgta gcagttgccg ctgaacgata cgcacagagc gcccgacacg aagaattcca
    33361 gttctacatc ggtagcctcg tgtatttctc ggatttggtc cagattcagt tcgcgggcca
    33421 gcactacccg acggatgccc gcgtcggcca gaaacttgac gtgcgctgca tcgcggttgt
    33481 tggtttgtgt gctggcgtgc aaggcgatgg gaggcaggtc catttctaaa atgcccatgt
    33541 cttgtatgat gagcgcatcc acgccggcct cgtacagttc gtggatgagt tgccggcagg
    33601 cctccaattc gttgtcgtag aggatggtat tgacggtgac caaaaccttg gccttgaaca
    33661 ggtgagcata cttcgtcagt tcggcgatgt cttccaccga gttgccggca ttggttcgcg
    33721 cgccgaaggt atgcgcgccg atatatacag cgtctgcgcc tgcgttgatg gcggccatgc
    33781 cctgatagag gtttttggcc ggtgcgagta tttcaacctt ttgcttcatg gtgcaaagat
    33841 gtaacaatcc tgattccttg cctcatcgga gcaaggtgat gtaccacaaa agaaagacgc
    33901 agcacacgcg ggttcaattc cggcctgtac tgcgtcagtc tatggtttgt aacttatgct
    33961 tcgatgggga gggaagctcc ccgagcgcaa ttacttgagg cgtctctcgt cggaaacgga
    34021 taacttgtgg cggccctgtc cgcggcggcg agccaatact ttgcggccat ctttgctggc
    34081 catgcgagag cgaaaaccat gcttgttggc tctctttctt ttagaaggtt gatacgtcct
    34141 tttcatgtcg gctcgtcttt aatatttact acgctttgaa aaaagtggca caaaggtacg
    34201 cctttcggcc atattttcaa ggatttataa aaaaaatatc ccccgcaatg aaaagtatgt
    34261 atttttggaa ggtgctttcc gctcttaaaa gttataacca cttatgaaac aactacttac
    34321 cgctgttttt atggtttatg gcgcactgct cgccgctcaa cctgtcaacg acaactgtac
    34381 cggcgtcctt gatttgggca ttgcgccggc ctgcccgcaa acagttttca ccaatgtcaa
    34441 cgctacggcc agcgacatcg gattcggcaa caatcccagt tgtttcaacg gcggcattgc
    34501 cccccgcgac gtctggtttg ccttcaccac caacgatact accctcgact attcgatcac
    34561 tgtgacgggg ttggccaacg gcggcttccc ggcgttgcgc aacccgccaa ttgcgctgta
    34621 ccggggcgat tgcgaggtca acgggctggc cgaaatccaa tgcgccactt ccgaccccgg
    34681 cgaaaccatt ctccgcttag acgtatatgg actgacgccc aatgtgactt attatgtgcg
    34741 catcagcgac tattcgccga ccgccacgcc caattggggt acatttgaac tgtgtgtgga
    34801 cccgctttca cctgttttca atattgacga cggcagctct acggcctgtt tcggccagct
    34861 attcgatacc ggcggccccg atgccgatta ttccaacaac gagacgcata ccttcaccat
    34921 ctgcccttcg gattttaata gctgcatcac ttttacattg gattattaca acatagagag
    34981 cggagatttc ggctcgcccg accaactctc ctttttcgat gccgctacgg ccgatccagg
    35041 cgcgctcatt accaccatcg gcgggccggg ctttcccagc gacggcggcg gcggcggcgt
    35101 gtgcttcccg attcaggctt ccagcggatg ccttaccgtc caattccgct ccgacggttc
    35161 cgtcaccgcc gagggttttg ccgggcattg ggaatgttcg accgtaccct gcgctcccga
    35221 ctcgcccatt actgtgcaaa ccggtattac cgaacaagac attctcaact ccatcacctc
    35281 tctgcaaacg cttgtggctg ttacaaatat caattgccct caaagcgctt acggcacctt
    35341 ccagtccacc gacaattcgc tggggcttcg gcggggtctc ttgctcacct ccggcgatct
    35401 gaactgggca gttgggccga acaacgatgg cggcggcggc aatctcaata ccgacaacgg
    35461 cgctcccggc gaccctgatc tggactacct ctcggttgca ttcggagacg gaacagaatc
    35521 caacgacgcc tgtatcattg agctggatgt aacggcattc actaatgaga tcacttttga
    35581 atacattttc gggtctgaag aatacccgga gtttgtagaa gatttcaacg acatctttgc
    35641 ttttctcatc tccggaccgg gcattgcagg cgatcccaat ataggcaacc agcaaaatat
    35701 agccgtactg cccgatggat ccaacaccat tgtggaaatc aacaatgtga acaatgtgac
    35761 caattggcaa tattaccgca acaacatcaa cggcgccgct acgcaatacg acgggcttac
    35821 ctccgatttg ctggggatca aaaaaagtct cactgcacgc gcatctgtca ttccctgcag
    35881 cacatatcac ctgaaaatcg ccattgccga ccgactcgat ttccggtatg actccggcgt
    35941 ttttctttct gaaattcgcg gcggaacacc ccgacttacg gctcaattca actcaggcat
    36001 tgactatctg atcgaaaact gtacgaatac gccggacaac gtggtggtgc aattgtccaa
    36061 cgtgttggat gaaaccgcca cttaccgcgt tcaggtaggc ggaaccgcca ttcgcggcgt
    36121 ggattacacg ttgaacctgc ccgataccgt cacctttctg ccggggcaaa cgcaactcag
    36181 cttccccatt tttcccctca acgacaacct tacggaagga atagaaacca tcatcattgc
    36241 cttgaccaac gatttcggct gcggcgaggt ggtttatacc acgctcacca ttgaactgca
    36301 cgatgaatta gtagtcaata tcattcccga tgcagacacc acttttgtat gccagggcgc
    36361 aacggctcaa ttggatgtag aaggcgcggc cacctatttc tggtcgcctg tcaacatttt
    36421 ggacgacccg ctcagccaga cgcctgtcgc cagccccacc gtcagcggat gggtgcgcgt
    36481 ggaaggcaac gtcggcatct gctccgatgt ggactctatc tatcttcaga ttattgatcc
    36541 ggttatttcc atcacggccc tcgatccgca gcttatttgc cagggcgaca gcgtaagact
    36601 ttccgttgtg gacaatgtaa acaataccaa cctgatatgg acgccggcca cgggcatcaa
    36661 tgatcccaac agccccaatc cggtcgtaac gccgcaggaa accaccactt acatcgccac
    36721 cgtcaggctg tttgaatgtg ccgtgtcgga ttctataacc attgatgtca atacgtttga
    36781 gtttcctcaa atcggcaacg acaccctggt atgccaaaac tacggggtgc aattggctga
    36841 aatgattgac gctgtggcca ccacttacag ttggacgccg acgaccgggc tgagcgatcc
    36901 cacggcttcc ggccccatcg ccacgcccaa cgtgaccacc acttacaccc tcattgctac
    36961 cagccaaaac ggcgcctgtg ccgacaccgc ccaaatcacc gtcaccgtag tgcctgccga
    37021 tgtggacatc cagcagcccg acaccctcga aatctgcctc ggaacggtgg tgcccatgac
    37081 cgctgtgacc agcaccggca atgcgcaggg tttgctgtgg agtaccaatc tgcccggcac
    37141 actcagcgat acggtggggc tgaatgtggt ggtaaccact tcccaaacag ccacctacta
    37201 tactcgcttc gaggtgggcg cctgtgtagt gttcgactcc gtttatgtac gggtggactc
    37261 gctgcctttt gaccgcgcca tcatcgccga tccggcaaaa gacccttatt gccccggcga
    37321 tatcgtcatt ttaagatcgg ctgtatttga gccgagcgac ttcccggata tcgaattcca
    37381 atggctgact gctcccggct tcgaaacgcc ggataccctg tggaatatgg tcatttcggc
    37441 aatagataca gctatttacc gccgggtcat taccaaccgg ggctgtttag ataccgtatc
    37501 catcaccatc aacgtggtag agccgcccac gccgaggatc ataccttctg atacgctcat
    37561 ttgcagcgga gaggcggtgc aacttctggc ggtagattac ggcgactatc ccatttcctg
    37621 gacgccgaca gccgggctga gctgtaccga ttgccccaat ccaatagcca cgcccaatgc
    37681 taccaccgtt tacaacctgc aagtggacgt acccaactgc ccggtggggg cttcggccaa
    37741 tatagtggtc atacctggcc ctacggtgcg catgaacaac gaccagacga tatgccgggg
    37801 cgcctctgtg cagttgaact tagattcgga tccgttcaca caatatcaat gggcatccaa
    37861 tgtggacgcg ggtttcagtt ccgccgatcc gctgctggta gccacgcctt tagtgactac
    37921 aacgtacacc ctcaccgcta cccggcagaa ttgcccgccg cgcgtcagga gcatcaccat
    37981 tacggtggtg cagccgccta cgctgatggc cagccaggat gtgacggttt gtcgtggcga
    38041 aagcacgacg ctgacggcta ccatcgtacc ggccaatgcg ggaggcgcct ttgcatggtt
    38101 tgcgcccggc agcggtacgc ctgccggcac tgcatcatcg ctgaatgtga attcggccaa
    38161 tctgggggct gccaaccgct atatcgtcac ttatgcttat ggcccgggca actcctgcgg
    38221 cccggtcagc gataccgtga tggtaacggt ggcagacccg gtacgaatag attccataac
    38281 ctccaatgta cctgatacca ccagattggt gatcggaaat gccattaccc tcacggcgca
    38341 taccacgccg ggcgatcctg ccggagctat gtatttctgg agtcaggacg gcggttcgga
    38401 gcaaatgggc ggccctgtat tcaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38461 nnnnnnnnnn nnnnnnnnnn nngacgggtg tatttccacc agaacctata cccgcgaagt
    38521 gatcgtgccg gatatcgcct tccccaacgt cttcacaccc aacggcgacg acttgaacga
    38581 cttgttccgg cccttgccca cgcaggtgtc gggcgtgctc atcatcgagg atttcaaaat
    38641 ttacaaccgc tggggacagg tggtgtataa cggcaagagc aacgacaaca tcggctggaa
    38701 cggcgaacac aacggcagtc ccgcgccgat agacgtatat gtgtatcggg tgaaattacg
    38761 ctacccggaa gggccgttgt ttgaaaaatc gggcgatttg actttgatca gataaaagcg
    38821 atgaactgtg agctgcgaat gatgctgcac gacggcaatt cgtagctcac ggctcgcagc
    38881 tttttatagt cacatacctt gctccgccag gttttgtgca tggccttcgg tacccgcgcc
    38941 atggcgcggt gattagcagc gcccggtatc gcacatgagg atgcgaggtg taattatctg
    39001 aattccgaaa caaaaaatat attgctcagg tgcccgtcat ggctatgttc gttcataaat
    39061 aagcaatata aggtaacatt atccccgttt tttaattctt tttttagcga tttttctaat
    39121 actttttgta caggcaacca gattcgatat ccttcaattt caaataaaca cttgttctta
    39181 accatttcgt tcagttggtc tgggttgccg atatacaatg tgaagacatt tttcatggaa
    39241 tttaatactt gagcgtctaa ttttttgaac tcgccaagat atttagcctt gattttatat
    39301 ttgtcaattc gggcataata ctgtgggatg tttttatctt gctcaacacg ctcagcgtat
    39361 tgtttttcag cattgcaaat gtctgtcaag ctaacttccg tatagttttt gtcccaagac
    39421 tcccactcat tttgtccaaa tgcttgaaga ttcaaaaggc aaataaggat gattaaaatt
    39481 ttattcatgt cgtttttgtt tagtggcata tcatatatac ctcgcatggt ttgcaagttc
    39541 aaaaatacag gatcggctgc tgttctctaa cttatgaggc aatgccaatc aacatggaaa
    39601 cgacaaacgc aataacgata agcacaatgg aatataccac aatagcagtg gtattagatt
    39661 ttccagtgtt cccattaaat gtgtgaccac aggattcaca tttcgttgaa ttaattaaag
    39721 caggacccaa tacgcctccc caccaagtcc attttactct tttaatggat tcgctgctgc
    39781 attttggaca gttcgtcatc atgttatgat cttttttatg ccctactcgt gtggcttttc
    39841 ggtttcgcct cgtttttata gtggtcgctg atctccactt ttgttttttt attggtaagc
    39901 tctgtgtgct cgttaaaatt gcctgtgatg ggtgcaggtt ttgcgaaggt gggtattttt
    39961 agcagtaagc tttcttgaaa aaagctgaac tacaaatata gcacaaatct ttctaaaagt
    40021 actaatcccc cgcttctgga tttttgttag ttactctttt tcaaataaaa atcttgttgt
    40081 cccgtcacta tgatttatta tttttttgtt ctttaaatta atagttaatt ccttttgggt
    40141 cagaataaat tcgtctaaaa agtctttctc tgtcattcct tttccaccta tgaattcata
    40201 tttacctttt ttcttttcag cttcaaagtt tcggaatgtg tttagcccat aagaaatgtc
    40261 gtgtagaatc gtattatttc ggattttcca aaatagaaat cttgttttaa gcacttctgg
    40321 ttcatcttca attttgaata ttgtcaattt atcaccattg aaggaataac ccaataattt
    40381 acctttatat tttcctgcgt ccaaactatc aagattaata gtttcagcat ctgatttagc
    40441 aaaatcgccc aactttgttt gaaaaacgaa gaagatagct ttgtttatgg agtatccaca
    40501 aacacctttg ttaaaaatcg aatacccgtt tccgtaaaat gctattacga aagtaagagt
    40561 tgctggaatt gtccaccatc ttgaaagtct tttaagtttc caaacacccg caatgaatag
    40621 agttacagaa cttaccatca taaaatatcc aataagtcga gaagtcaaaa caggataata
    40681 gacactacaa gttgaactac aaccacagcc aaggtcgcta taaaaaaagt tttcaaggga
    40741 ataatagaat agtcctaaca aagttgtcaa gattaacgtc agtgcaattc ctatcttttt
    40801 tgttcttgtc atttagttga tttcattcta ttgtgtgtgg tttttagcct gacggctaac
    40861 gttcccatgc tttgagtccg ggcggttttc ggagtacaac ctgtcccgcc cttgcgggaa
    40921 aactccacgt ttgcactgct tgccgcgcga ggaacgaaac gggattaccc cgccggatgc
    40981 aaaaattgtg ttggcattct ttattagaca tgttgcgcca ctgttaatca attagttata
    41041 taaaaattct ataaaccggc aataactccc agtatgtcat catacaaatt aaaatcatgc
    41101 attctggccc tatccgacaa tattctgtat cgtttcattc ctccaatact gtgttctaca
    41161 acgattcgtt ctcgtgaaac actcttgttc tctaactttt gttgttcgtc taattcttta
    41221 cccttgctct ttttgtgagg aattccaaca cattcacact cataattttt atcaaaccct
    41281 gcaaacccta aatccaactg aacctcatga tttttgaacc aagcctcttg gggcgcaaat
    41341 tcctctttaa gtatgcaaaa atcatgactt tttcctaccc agcacggact tacatagtga
    41401 atgtacttat cggaagtgct gataatcaat gtttttactg tgtgactttt tttttgccac
    41461 tgtacatcgt tttttgtttc tctttattct taggccgctg aactcgttgc tccgttccat
    41521 ccaaaattag ttttttgtgg ccattgaaat attcttgaaa ctcttctacc gtatcaaagg
    41581 aacgcttggg catacatcct atactatcca gcgcttccga caatattctg attcctatgc
    41641 tctggttcct tttaacattg gacgggtcta ttcctatcaa gtaccccatc atgtcatatc
    41701 ctaaccctga cttcaaactc cacagcgtga agaacaactg atcgtgatat gtcttaaagg
    41761 tactgtcccc ggggcaatct gctaatcgct cctctatacc ccgaccgtaa tgtgcttcat
    41821 aggccttctt gtataattca agcaatacat taacaatggt tcgtgatata ctcacgccgc
    41881 ccaggcggcg taataattag aaaa
//
LOCUS       DAORLF010000003        41852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_479_length_41852_cov_2.098380, whole genome shotgun sequence.
ACCESSION   DAORLF010000003 DAORLF010000000
VERSION     DAORLF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 41852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41852
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_41852_cov_2.098380"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..810)
                     /gene="nqrE"
                     /locus_tag="PK198_00735"
     CDS             complement(196..810)
                     /gene="nqrE"
                     /locus_tag="PK198_00735"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRF37285.1"
                     /translation="MELFNIFLKSAFIENMVLAYFLGMCSFLAVSKTVKTAVGLGLAV
                     IFVLGVTMPINWLLNEYLLTERGIFGTDLTFLRFILFISTIAAMVQLVEMVVEKVSPS
                     LYNALGIFLPLITVNCAILGGALFMVAREYTLAESTVFGIGGGFGWFLAIVALAAIRE
                     KIRYSHVPPALRGLGMAFILTGLMGMAFMSLMGINPAELMAAGK"
     gene            complement(874..1605)
                     /locus_tag="PK198_00740"
     CDS             complement(874..1605)
                     /locus_tag="PK198_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRF37286.1"
                     /translation="MADTATKTMSVQEKEPWFGKKERKLLTDPLEGNNPITVQVLGIC
                     SALAVTTQILPALVMAIAVTFVTAASNVTVSALRNYIPKQVRMIVQLVIIATLVTIVE
                     LALKAFSYPVWKQLSVFIGLIITNCIVMGRLEAYAMANKPWKSFLDGIGNGLGYGAIL
                     LIVAFFRELFGKGTLFTGTPIALNIIGPSNELTHYWINTADPNSWLYGYTNNGLMVLP
                     AAALFLVGILIWIQRSWNKSLVDVS"
     assembly_gap    1702..1866
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1867..>2136)
                     /locus_tag="PK198_00745"
     CDS             complement(<1867..>2136)
                     /locus_tag="PK198_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRF37287.1"
                     /translation="EVSGQLAEKINLAQEKKKPEADRLLPVFVYKDAQGKPYYILSVR
                     GNGLWDEIWGSIALDSDMNTIAGVSFDHKGETPGLGAEIKDSPAFP"
     assembly_gap    2137..2332
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2449..3720)
                     /locus_tag="PK198_00750"
     CDS             complement(2449..3720)
                     /locus_tag="PK198_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRF37288.1"
                     /translation="MKKALLKFFSSIEPDKKKSPFLHTLYDGFFTFALTPDHVTKTGA
                     HIRDGMDLKRTMVFVVLAMQLCYLFGMYNTGHQHFAALGQYVGLWDGWHLKLVHGLIK
                     LLPIIIVAHVVGLGIEFYYAYKKGHGIEEGFLVSGALIPLIMPPDLPLSILALSVAFA
                     VIIGKEAFGGTGMNILNVALLSRVFVFFAYPGHISGDEVWISGLEKVNGAYAGEMYSW
                     AHHLFDGIFKGLGWETFGAGGAAVIDGYTGATPMALAAKGGWEAVTKVYNPSDMFWGL
                     IPGSVGETNKPFIIIGALMLIATGIASWRIMVSMVLGLAFTGLLFNAWGATPFMEVPW
                     YYHFSMGSFLFAMAFMATDPVTASSTNVGKWVYGFLIGVIGLIVRVLNPAYPEGWMLA
                     ILFMNVFAPLIDYVVVQGNIKRRTKSAAAKA"
     gene            complement(3777..5339)
                     /locus_tag="PK198_00755"
     CDS             complement(3777..5339)
                     /locus_tag="PK198_00755"
                     /inference="COORDINATES: protein motif:HMM:NF003761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRF37289.1"
                     /translation="MKNTTLKFLLLLVTWGIASGDILAQSAGSSGSKSMYALYAIGAL
                     LLTGIWMQVSDKFAGKKSAGGAQDGSTVSLQQGHDIILKGVPAKTQVAAQAKTYAVCP
                     PVFKGLSPIPKMEVEVGAEVKAGDALFHAKSAPSVKFVAPVSGEVIAINRGAKRAITE
                     VVILADKEQQHRALTPPQLNSCSRQELQDFLMDSGAWALIRQRPFQIVPAPGDVPRDI
                     FISTFDTAPLAPDLNHVVEGKGEAFQKGLDVLARLTDGSVWLGLDGRGKSAPSAVFAQ
                     AKGVKKVSFKGKHPAGNVGVQIHHINPIKSGEKVWTLNVQDVVTIGALFTEKRFNAAR
                     LVALSGDECKTTGYVSTHIGANIGDLTTGNIKGENVRIISGDILSGATTNAAGFLSPF
                     DDQITIIEEGNDFEMFGWLAPSYARPTVSRTYLGNLVAPGRSFHVNTNTHGEKRAFVV
                     TGQYESVLPMDVLPQHLFKAILAGDIEQMEGLGILELSEEDVALCEFACTSKQSLQQI
                     LQDGLEMMQEQM"
     gene            complement(5498..7030)
                     /gene="hutH"
                     /locus_tag="PK198_00760"
     CDS             complement(5498..7030)
                     /gene="hutH"
                     /locus_tag="PK198_00760"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRF37290.1"
                     /translation="MPPDFLTIYTKHNIMHRISHERISPEQAQHWIESRLPLELATEA
                     TQSIIACRKYLERRMADSDQLLYGINTGFGSLCNIRISDDETEQLQHNLVKSHACGMG
                     EEAPVEIVRAMLFLKIRNMAYGHSGVRLELVQRLIDMYNADVIPVVYQQGSLGASGDL
                     APLAHLSLPLIGLGEVWYQGRKRPAEEVLAELGMAPLRLQSKEGLALLNGTQFSTAYA
                     LWAVNKAQQLFATANMCAALSADAFQCRLSPYDQKLHIVRAYDGQIRTAAAIRHWLAG
                     SEIAAQEKAHVQDPYAFRCVPQVHGASWEAIEHVRHLVQTEINSVTDNPMIFPDADEI
                     LSGGNFHAQPMALALDYLAIAVSELGAISERRTYQLISGARGLPPFLTPLPGLHSGLM
                     IPQYTAASIASQNKQLCTPASADSIVSSNGQEDHVSMAANAATKAWRVLNNVQSILAI
                     EWMTAAQALYLRQPAQSSPALEKQVEAYRKLVPPLREDRVLSDDMQATVQFLADAKIH
                     AA"
     gene            7210..7743
                     /locus_tag="PK198_00765"
     CDS             7210..7743
                     /locus_tag="PK198_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF37291.1"
                     /translation="MTFYSLFRNGKILALALGFTLAFSTAASAQRIAYVDVNRILESF
                     KEYQDAQSELDRVASKWRQEIAQEYDVIKGLYNRYQAEQVLLSEDARRKREDEIMNRE
                     KEVRDLQKARFGPEGELFRRRQEMVRPIQERVYRAIESYATERGYDFIFDKSGAAGII
                     FFSPAYDKTDDVLSRLK"
     gene            7805..>8083
                     /locus_tag="PK198_00770"
     CDS             7805..>8083
                     /locus_tag="PK198_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF37292.1"
                     /translation="MMKHLLKTGLALLLMFGLTQTMSAQKFGYINSSMILAELPEVKG
                     AEATLEALQQQLQKKGQSMLEKLQADYAVVQQKVERGELSPKQQETEGA"
     assembly_gap    8084..8093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8094..8315
                     /locus_tag="PK198_00775"
     CDS             <8094..8315
                     /locus_tag="PK198_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF37293.1"
                     /translation="AAEEEIGKFEQNMMAQLENKRNELLKPIYDKVNQAIKDVAKENG
                     FQFIFDQGVLLFAEESQNVAPLVKSKLGI"
     gene            8330..9136
                     /gene="murI"
                     /locus_tag="PK198_00780"
     CDS             8330..9136
                     /gene="murI"
                     /locus_tag="PK198_00780"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRF37294.1"
                     /translation="MQHSPNQPIGVFDSGIGGLTVANAIMRALPQEEIIYFGDTAHLP
                     YGEKSADAIRYYSLRIAKFLLERHCKMVVIACNSASTAAYRVLLEFFKGQGLFVNVVD
                     PLVESVAARRFQKVGLIATKATVQSGIYEQQLLHLQPGLEVHSLATPLLAPMIEEGFV
                     HNDVSQVVLESYLGHEQLQGIEALLLACTHYPLIKTEIENFYHHQVEIFDSTDVVATE
                     VKRKLAEHKLLNEQRRHAHRFFVSDYTPTFEQTTRIFYQEDIVLEHYPMW"
     gene            complement(9137..9514)
                     /locus_tag="PK198_00785"
     CDS             complement(9137..9514)
                     /locus_tag="PK198_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF37295.1"
                     /translation="MKPQISISKQLMAQYDLANTVQGGMAQGGAKAWQDKAGFHLELR
                     TPGIPLEKIRIEAVNRRFVVYYMMEVLSGEVELPHYLVNLPLLPIVDVERIAAKVEEG
                     QIHIFAPFNDWAKGSRIEIGLDG"
     gene            complement(9612..10847)
                     /gene="hutI"
                     /locus_tag="PK198_00790"
     CDS             complement(9612..10847)
                     /gene="hutI"
                     /locus_tag="PK198_00790"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRF37296.1"
                     /translation="MLLLRNIQTIFQTETAPLRERVAGADMQVLPSVDDAFLLVENGK
                     ILSFGPMTQCPDAPGAQVVDAAGRLLLPTWCDSHTHLVFAASRENEFVDRIHGLTYEQ
                     IAQRGGGILNSARRLRDTPEEVLLELAWQRLLEVQRYGTGAIEIKSGYGLSVEGELKM
                     LRVIRALRERTKMPVKATFLGAHALPAEYKTDREGYLRLIEEEMLPRIADEGLADYID
                     AFCEKNFFSPAETARLIEAGARYGLKAKIHTNQFNCLGGIEVCVERGAVSVDHLEVLT
                     DEEINCLKNSDTLPVLLPSAPFFLNDHYPPARRMLDAGLPLVLASDFNPGSSPSGKMP
                     FVVSLACIKLRMTPEEAVNAATLNGAAAMEISHSHGSIAVGKSANFFLTKPVSSLAFL
                     PYAFGSDVVERVWIAGEEV"
     gene            complement(10875..11189)
                     /locus_tag="PK198_00795"
     CDS             complement(10875..11189)
                     /locus_tag="PK198_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37297.1"
                     /translation="MGLSLLLLVGGVILFGYERFGNAAILGFIVGNVIDRAIICALYS
                     SYIALGTMVVPIMSSLIMAVVWYKNREVQDLKKPYLLMVLVLVLLIIVHPKLVEGPMW
                     PW"
     gene            11399..11839
                     /locus_tag="PK198_00800"
     CDS             11399..11839
                     /locus_tag="PK198_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37298.1"
                     /translation="MVSKDHLARLAEIVQHPDKWEDSTKYFLDHFASDQEFMEMGKRT
                     RIDVLLKIMGEVAKKVLNKSLVRIENPQMYRVKSMNMIHGSCTSEGWVMMFVYFTDIH
                     KGVFSTTKPFSGQTGFSSRVTALPMPELDNEERLYGDQWNMNPN"
     gene            complement(11840..>13316)
                     /gene="gcvPB"
                     /locus_tag="PK198_00805"
     CDS             complement(11840..>13316)
                     /gene="gcvPB"
                     /locus_tag="PK198_00805"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRF37299.1"
                     /translation="PCLTHPVFSAYHTESKMMRYIKRLENRDLSLTLSMISLGSCTMK
                     LNAATELMPVSWPEFANLHPFVPADQAGGYRRIFDDLEAWLSDITGFAACSLQPNSGA
                     QGEYAGLLTIRAYHEARGENHRNVALIPSSAHGTNPASAVMAGLDVVVVNCDDHGNID
                     VDDLRAKAEQYRDRLAALMVTYPSTHGVFELRIHEICDLIHDNGGLVYMDGANMNAQV
                     GLTSPGNISADVCHLNLHKTFAIPHGGGGPGMGPICVNEKLKPFLPSHPLVGTGGEQG
                     IDAVSAAPWGSASILLISYAYIKMLGPDGLTDSTRYAILNANYIKSRLENDYSILYVG
                     DLGRTAHELIVDLRPFKNLVSAEDVAKRLIDYGFHAPTLSFPVAGTIMIEPTESESKD
                     ELDRFCDALLQIRKEIDEIASGEADVQSNVLHNAPHTADLVTADDWPYAYSREKAAWP
                     LPYLRQGYKFWASVGRIDNAYGDRNLICTCPPIEAYADSEA"
     assembly_gap    13317..13341
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13342..>13689)
                     /locus_tag="PK198_00810"
     CDS             complement(<13342..>13689)
                     /locus_tag="PK198_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRF37300.1"
                     /translation="YAAYHGPQGLRAIAERVHTLAAILADNLQSLGYHLPNAYYFDTI
                     FVETDGKMQEQLREIALKHEVNFYYPATGGVQISLDETTLLKDVEQIVRIFAEAKGAA
                     HAFSVAEPAQLRVP"
     assembly_gap    13690..13700
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13701..>13953)
                     /locus_tag="PK198_00815"
     CDS             complement(<13701..>13953)
                     /locus_tag="PK198_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37301.1"
                     /translation="GEWGADAVVGNTQRFGVPMGYGGPHAAYFATRDDFKRVIPGRII
                     GVSVDRHGKRALRMALQTREQHIRREKATSNICTAQALLA"
     assembly_gap    13954..14065
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14066..14729)
                     /locus_tag="PK198_00820"
     CDS             complement(<14066..14729)
                     /locus_tag="PK198_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRF37302.1"
                     /translation="MSKISFFDRFANRHIGINEGELQDMLAVVKADSLDQLIYETIPD
                     SIRLQRPLNVPPAESEFEYLQELKAVAAQNKLYKTYIGMGYYGTITPSVIARNIFQNP
                     GWYTQYTPYQAEIAQGRLEALLNFQTMVSELTGLPIANASLLDEGTAAAEAMHMFYEQ
                     KNKRAKGEPANEFLVSDQVFPQTLSVLKTRAKHLGIKIVVQPDNKLEINDKTFGILLQ
                     YPG"
     gene            complement(14981..15382)
                     /locus_tag="PK198_00825"
     CDS             complement(14981..15382)
                     /locus_tag="PK198_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37303.1"
                     /translation="MFFTKTGKFTEEEFDVAHRQFQESGGRQPQIFTFFKNGDIKMGS
                     ITNEVKTLLDFKEKLRALEHFYTSYENLSDLKLRFRDQLDKMERYEQMALPGAPPEIC
                     CLRFDKFQKFVKSCLAPSEGLKPKDGFQLSG"
     assembly_gap    15445..15533
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15796..15808
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15809..16748)
                     /locus_tag="PK198_00830"
     CDS             complement(<15809..16748)
                     /locus_tag="PK198_00830"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRF37304.1"
                     /translation="MSEIKGKIRTAQIRAMVSANREMLFLYWDIGKSILERQQQHGWG
                     SKVVDQLGEDLRVEYPEMEGLSKRNLLYMRQFALAYPDFEFVQASLAQISWYHNITLL
                     QKCKDEIKRFWYAAAALANGWSRDVLVHQIESKLYERQGGAPSNFQQTLPRPDSDMAQ
                     QTLKDPYIFDFLTLAKEAREKDLETLLMKHITRFLLELGSGFAFIGNQFPISIEDDTY
                     KIDLLFYHLKLRCYVAIDLKMGKFKPADAGQMNFYLSALDASMKSPIDNPSIGIILCK
                     DKKNVVVEYALRDLNKPIGVASYLLADALPDNLKSSI"
     gene            complement(16804..16962)
                     /locus_tag="PK198_00835"
     CDS             complement(16804..16962)
                     /locus_tag="PK198_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37305.1"
                     /translation="MSELAQQLIAENKAKHARGEDASYLYLGRCGLTELPDLSGMEWL
                     ETHLKIQV"
     gene            complement(17166..17588)
                     /locus_tag="PK198_00840"
     CDS             complement(17166..17588)
                     /locus_tag="PK198_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRF37306.1"
                     /translation="MTILIDSNLWVASFISSSMRERIQRLVSDENIVILADSELLAEL
                     ADVFSRPKFSKIARSELTAAFVQILKDRLTFIETESQFQICRDPDDDYLLAICHDGKA
                     DYLLTGDNDLLTLIQFEQTRIVTLSDFEQLFYSSPPLS"
     gene            complement(17594..>17801)
                     /locus_tag="PK198_00845"
     CDS             complement(17594..>17801)
                     /locus_tag="PK198_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37307.1"
                     /translation="DISLEQIMDALMELPASEKLQVAEKLRAAAAGEKWRLLSPQLPD
                     MPDISMDEIVAELKSVRKARKQSQ"
     assembly_gap    17802..17824
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17999..18601)
                     /locus_tag="PK198_00850"
     CDS             complement(17999..18601)
                     /locus_tag="PK198_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF37308.1"
                     /translation="MAFFKIVLLSSFVAFMAGLETAETSYYIDAGSSLSIAGSSNVNK
                     FTCNCSEQFSKGSIRFELQDGGKTLRFSHASLHLRSKSLDCGNPQMNKDMYKTLRADQ
                     HPGIRIELTRAQLQEAELATGKDWSPLKASSQLTIAGVTQSVVFDVKAKRIAPDRIRL
                     TTSKEVLMTDFGMEPPTAMLGLVKVNNTIRINMDLIVVVV"
     gene            complement(18757..20142)
                     /locus_tag="PK198_00855"
     CDS             complement(18757..20142)
                     /locus_tag="PK198_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37309.1"
                     /translation="MKASTLRLLVALFVLAISFKATAQQPKLQYFRGWDKDAINVFEP
                     TKADDTPYEGFKIRIGGSFTQNFQSLTHENNPTYVPVSTTNPTNRNLLYGVIKAEDSL
                     SVNLQGFNLAMANLNFDFQIEDGIRVCLENYMSARHHNEFWVKGGYIQIDKLPMFGNP
                     QWFSDYFRVKIGHFQPNFGDMQFRRSDGGNTMFNPFVENYILDAFATEIGGEVYAFLP
                     GGFMGMVGMSSGFINGNIEKYTSTPQGTNKEVTKKTPSVFFKLAYDKTFDNDFRFRLS
                     ASMYNNPSIQRNTLYAGDRTGSQYFLMMDPALTNGAATTATAQFTSGRFNPGLTNQIN
                     TVMINPFIKFKGLEVFGSYEIAQGRTYAEDLDAKRKFTQLAVEGVYRFLPNESLFVGA
                     RYVQASGRPQGFTDDISIDRLAFAAGWFPSKNLLLKAEVVNQNYKDFPTTDYRNEGKF
                     NGFVIQAAIGF"
     assembly_gap    20195..20251
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20252..20484
                     /locus_tag="PK198_00860"
     CDS             <20252..20484
                     /locus_tag="PK198_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37310.1"
                     /translation="YDFNHLRNRAGMTLQANAERRAAYQASEVKDKKNRSLEINLLIF
                     GLVLKTYFVQYPAETGQQMQQNDDEKYIGHDD"
     gene            20821..21339
                     /locus_tag="PK198_00865"
     CDS             20821..21339
                     /locus_tag="PK198_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37311.1"
                     /translation="MTSFDNLLLISGAGRKTFKTTFACEVIRLHRNKGVVGVKISSHF
                     HPQEGAVHWKAVPGQYEIYEESRPDGPRDSHRMLHAGAEKVYYIQCRKEALAEAFQSV
                     LAEFSPGQPIVCESGGLADVLRPALHLALKGDGTKALHPLADVMIEKDETGFSVSPER
                     IVFENGKWGIAA"
     gene            complement(21424..22008)
                     /locus_tag="PK198_00870"
     CDS             complement(21424..22008)
                     /locus_tag="PK198_00870"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF37312.1"
                     /translation="MKKLIISGIFMLTTSMFLFAQSAYQVKSFKMTVAGTSTLHEWES
                     KVNKVTAKGDIMADVNAIQSIKNLSVEVDAKSIISTKGKVMDTKTWEALKADQHPKIT
                     FKLSKVESISKSGAEFVIKTTGNLSIAGVTKSVPMEAKGKILANGDLEFSGSKLIVLS
                     DFGMEQPTALMGTVKVGKEVTVKFSVVMSSAAVQ"
     gene            complement(22211..>22376)
                     /locus_tag="PK198_00875"
     CDS             complement(22211..>22376)
                     /locus_tag="PK198_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF37313.1"
                     /translation="KKAMEKLQLSARAFDRILKVARTAADLADSPDIKIEHLAEAIHF
                     RSLDREGWAG"
     assembly_gap    22377..22453
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22454..>23564)
                     /locus_tag="PK198_00880"
     CDS             complement(<22454..>23564)
                     /locus_tag="PK198_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRF37314.1"
                     /translation="VNLAPADIRKEGSAYDLPIAIGYMAATDQLVCDDLERYLIMGEL
                     SLDGGLRPIKGALPIAIQARKEKFKGIILPKQNAREAALVSDLEVYGVENLSEAVGLL
                     NGDAPLQPVVVNTREEFFNNVSNYEVDFSDVKGQENIKRALELAAAGGHNAILIGPPG
                     AGKTMLARRLPTIIPPLSLHEALETTKIHSVAGMLPSNAALITTRPFRAPHHTISDVA
                     LVGGGSNPMPGEISLAHNGVLFLDELPEFKRSVLEVLRQPMEERRVTISRAKTSVDYP
                     AGFMLIASMNPCPCGYYNHPEKECVCGPGVVKRYLSKISGPLLDRIDLHVEVTPVSYD
                     ELASRERPSETSADIVQRVIRAREIQAERFKDRTDV"
     assembly_gap    23565..23589
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23590..23775)
                     /locus_tag="PK198_00885"
     CDS             complement(<23590..23775)
                     /locus_tag="PK198_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF37315.1"
                     /translation="MLVKTFAAAVHGVDAQTITVEINTGGNVAVGSQFYFMVGLPDSA
                     VREGSQRMEAALKNSGFK"
     gene            complement(23848..25713)
                     /locus_tag="PK198_00890"
     CDS             complement(23848..25713)
                     /locus_tag="PK198_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRF37316.1"
                     /translation="MKRLLYFFLCLVPAALFAQSNALDSAWIRENYTKSERYVPMRDG
                     VRLFTTIYAPKDATEKHPILMNRTPYSCAPYGEDNWRDMWNSHWKYYLREGYVIVIQD
                     VRGRWMSEGEFVDVRPFNPNKKTPQDTDEASDTYDAIEWLLANVPNNNGRVGAFGISY
                     PGFYSTMAALSAHPALKAVSPQAPVTDWFMGDDFHHNGAFFLMDGFSFYSGFGKPRPK
                     PTTARPEGFPFSVEDNYKFYLETGALKYFKTKLLGDSIAFWNDLMEHPNLDDWWKARN
                     PRNHVTNVQPAMLVVGGLFDAEDCYGAWRLYEAIEQKSPRTDNRIVMGPWAHGQWARQ
                     PGDQLGHIRFGSNTAHWYLNHIEMPFFNYHLKGKGSLEKLAEATIFFTGANRWEHLPQ
                     WPPVAKTDRPMYLQADGKLNWNKPAAANSFTEYISDPAKPVPYAEGVHLRRTREYMLD
                     DQRFAARRTDVLVFETPVLAEDLTLAGTVIADIKAAISTTDADFVVKVIDVFPDDFRY
                     EEGSEYPMGAFQMLVRGEVMRGRYRNSFEKPEAFTPNKVETVRFELPDVAHTFRKGHR
                     LMIQIQSSWFPLVDRNPQQFVNIYTCDDSDFKKSTIRIFHDAKHASSIVLPVWKP"
     gene            complement(25741..26304)
                     /locus_tag="PK198_00895"
     CDS             complement(25741..26304)
                     /locus_tag="PK198_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37317.1"
                     /translation="MHFTHRLLPLLTALFLLSGTAMYAQETSKFTDKLWYGGGVNLGI
                     SGSNGFNQFGLGISPMVGYKIGEMFSVGPRVALNYTHIRVRLFNNEIATANPLSWSAG
                     VFGRAKIIRTIFAHVEYELEEQALAFISGNDLVIQRQQRNNTYIGAGYNSGGIWASEI
                     MLLYNVTPVVTDLRSPFVVRFGFTYKF"
     gene            complement(26359..27393)
                     /locus_tag="PK198_00900"
     CDS             complement(26359..27393)
                     /locus_tag="PK198_00900"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRF37318.1"
                     /translation="MPAPAMYRLVFQPLLFLLSPEKAHAVALALLELLLHIPGAESLL
                     RRSLNRSSPLTVAGIRFPNPVGIAAGFDKNGKHIRALAALGFGFIEVGTVTPRPQAGN
                     PRPRLFRLPADRALINRMGFNNDGLEALIRQLQRPRPAGIVIGGNIGKNKDTPNEQAA
                     DDYIACFEALFSWVDYFAVNVSSPNTPNLRALQDREPLTRLLAELQQRNDNQANPKPI
                     FLKIAPDLTDAQLDDIVAIVRETRIAGIIAANTTISRTGLKTPAETTERIGAGGLSGY
                     PVRERSTQIVRYLRAQLPPPFAIIGVGGIDGPESAREKIEAGADLVQVYTGFVYAGPT
                     LAADIVAKLK"
     gene            complement(27586..28308)
                     /locus_tag="PK198_00905"
     CDS             complement(27586..28308)
                     /locus_tag="PK198_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37319.1"
                     /translation="MANNRILLVEDDQNFGDVLKSYLEMHGYDVKLATDGLQGSETFK
                     KGRFDLCILDVMMPKKDGFTLGKEIRDKDAEMPVIFLTAKTMKEDVLKGFKIGADDYI
                     TKPFNSEELLYRIQAILKRSQPKADLRDDIKEFNIGKYHFNYPLRILSYNMGGAGEEK
                     FKLSPKEAQLLRMFALNANDVLPRATALTEIWGQDDYFTARSMDVFVTKLRKYLRLDE
                     NIEIVNIHGNGFQLLIKAQEKI"
     gene            complement(28322..>28770)
                     /locus_tag="PK198_00910"
     CDS             complement(28322..>28770)
                     /locus_tag="PK198_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF37320.1"
                     /translation="FGIRVEQRQGRLDGKLEAARPVIQGDLTHLSNIVNNLLDNAEKY
                     SPDSPEITVLTRDVKGGVEITVQDKGMGMSKEARKHIFDKFYRVHTGNVHDVKGFGLG
                     LSYVKAIVSAHKGSIEVKSEPGKGSSFIIFLPHQADTGRAGKSLHP"
     assembly_gap    28771..28786
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28787..29844)
                     /locus_tag="PK198_00915"
     CDS             complement(<28787..29844)
                     /locus_tag="PK198_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF37321.1"
                     /translation="MNKKYILAVIGLMSAAVVGVVWLQMDLINTAIKENEVKFDKDVF
                     DALNSVVIRLEDEEQAEAYEYYINGYGAAYFDNEVRSNRVPGASAVQANALQNWQLPV
                     NSEEEQCDCAKCVRERNDINRSMVSFLKLQGGQQTPIAERIKKDKLRDILRQELANRG
                     INTGYNYGLLATASNGFVIMDDRYVVESSEPRESLPGYSNIYNSKYRVHLFPNETPAP
                     GLLMIHFPARSEFVGRSVLPNFIGALIFTAIILFSFGYTIFVIFTQKKLSEMKNDFIN
                     NMTHEFKTPIATISLAADSINSPMISGDADKVRRFANIIKQENKRMNSQVEKVLQMAL
                     IDRREFSLNLSEVNLHEV"
     gene            complement(29837..>30038)
                     /locus_tag="PK198_00920"
     CDS             complement(29837..>30038)
                     /locus_tag="PK198_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37322.1"
                     /translation="GLAAIIGWSSIITFCILQKELNADSGCCVSKAGAEFQYKGRKTS
                     AAGISFLCRVARGSLHIYTSYE"
     assembly_gap    30039..30057
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30061..30726
                     /locus_tag="PK198_00925"
     CDS             30061..30726
                     /locus_tag="PK198_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF37323.1"
                     /translation="MKGENSWTMFKMISEFVDGFETLNQIGPCVSIFGSARTKPDHPY
                     YRKAVEIASVLTKAGYGIITGGGPGIMEAGNKGAALSGGRSVGLNINLPHEQSHNQFI
                     DPDKNLNFRYFFVRKVMFVKYAQAFVILPGGFGTLDELFEVLTLVQTKKITPLPIILF
                     GTEFWGGMREWIRTVMLEKEHNISPGDMDLIPMTDDAEEVVRIINDFYEKRLSELGPN
                     YEL"
     gene            30726..31583
                     /locus_tag="PK198_00930"
     CDS             30726..31583
                     /locus_tag="PK198_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF37324.1"
                     /translation="MIRYHDVNGGLRQKYTASFRLDDLGLLRGYGIFDYFLVRQGVPM
                     FWDDYADRFYRSARLLGLEVPIAPEELRQRIARLLEANRERNTAIRLLLTGGYADDSY
                     TPAEPNLAIMQHPMPSVAPERYEEGIKLMLYRHLREVPEVKSTNYLTGIHIRELLKQA
                     GAPEVLYHDGRLVSESARSNFFIVTAEGVLVTPAEQVLHGVTRKHVLELAQQAGIRTE
                     VRDLTLDELPRAAEAFLTSTIKGVLPVVQIDELRIGEGRPGPVSRRLRELWQNHVADY
                     IARHQPFKR"
     gene            31580..31927
                     /locus_tag="PK198_00935"
     CDS             31580..31927
                     /locus_tag="PK198_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37325.1"
                     /translation="MNALRRKLLIALCCCLLGGAALSAQTVRRASGPGLQLPPLHVSR
                     MSWRVPAPELPVLPNAKFQPAAQPKAWHYQELALFCKLEVKMERAFRMPLRLRIGDVQ
                     YVDWLEGKRRSAY"
     assembly_gap    31961..32101
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32336..33673
                     /locus_tag="PK198_00940"
     CDS             32336..33673
                     /locus_tag="PK198_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF37326.1"
                     /translation="MKYIFYVAITLLFSGIAAKAQTPLSIEQAIALGIQRNLPLQSAR
                     NNAETARINNDWGAAGRYPDVNATVNLNNGYNNNQNPASFLQSLSALNTGITPGVEAV
                     WVVYGGHRVHISKEQLQLIENQAQGNVRQAVEATIEDVILSYYQAVIQQEQLQVRAEV
                     LRLSRERLEYQRTRQEFGQSSTFDVIQTQDAWLNDSTAYLVQQTNVANAMRNLRRAIG
                     EDNPETAYALTDRLPVETTRYDAEELRTRMLNSNQALAQLRIGRELAAVQTRFQESFN
                     KPTLSLRSGASYTLSRNIFANGLFANGELRDLGGIQNTNFNLFVNLSAAYNLFDGGAR
                     RRNVQTARIAELNTQLGMDDMRRNLLTQLDIALESYNNQKRVLELSNQLVNNARRNIQ
                     IAEERFRGGLISFFDYRSIQISYINATQTRLNALYNLKTTETELLRLSGGLVR"
     gene            33782..>35408
                     /locus_tag="PK198_00945"
     CDS             33782..>35408
                     /locus_tag="PK198_00945"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37327.1"
                     /translation="MKPLQSLFFFTCAFCLISLQTTHAQNFRAVDDTVCIQLGVPFTF
                     NVKDNDSIPAGGNALVVPLFQSNCVRLLENGELRLISDSPDCCGTYTFGYRYENCDDP
                     ACFAKIELTIKCPKPDCSVIDLSDYLNRSSSGNPDLQPACVDVCAESEVTFFLPNLPG
                     FSYNWSASGGVVSPGSHPAEVNIVWGPAGTGNISITITDSLNVGITRNICVNILPKPT
                     ASFTKSATSICLGGSVTFTNTSTGGNSYYWDFGDGSSSAMFSPTHEFNSPGTYDVCLY
                     VTKDNVDPRGNPLCCCTDTMCMTVTVDSLPGPNIYWISTLCAGDSSKYWTDAANCASY
                     QWTVLDHNGLPLSFTGQGNDTICVVWGNGPFGTVTLQVSGCSQTYCDEPVTAVVPIIS
                     PTAVVSGPTVVCQNETATYTLPKWPGVYYDWQVGGGTVVSGQGTNVATIQWGPGPTGT
                     ITVNYSSPFLQGLPGHSPPDCSGTATLNVQIRPKFTVQASPSVVCVNSTSSVFALPFT
                     SYNWTVSPAALFSGQGTGAININWTIPGTYTVTA"
     assembly_gap    35409..35430
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35431..>39755
                     /locus_tag="PK198_00950"
     CDS             <35431..>39755
                     /locus_tag="PK198_00950"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37328.1"
                     /translation="YCNSSASVVIRVLDVPAPDSITGPLLICPGETQTYFGHTSASGT
                     VLNWTVTGGTPTTGTGNSITVTWGGSGLYTLSLSQSLTGQPMCSSPAIQLTVQAKQIA
                     GPLTISGPSGCINSTQMYSAGPAQHPDAMFNWTISPATAGSVVAGQGTPNIDVQWNNT
                     PGTPMLTVAVTLCGVTQTHSIPVTLNPPVVPVITQTGVLCPGSSVTLDAGSGFSGYLW
                     SNMATNQTITVSTPGSYTVKTIDANGCMITVPYQVVPAPGPLASISSGSPTLLCINPP
                     TPASSTLIALTQPGNTYEWFCNGVSQGASSPTATTFTHNNTNVAATFNYWVVVTAPNG
                     CISTSNSITVVQDICDGDGCTPNGPHSLSFTPANQFPNCNVVNFSTTSSNVTLTGWNF
                     GDPGNSGTLAAAQHTYTQAGCFYVELLGTTPGTLSDGTPANCLVGFTDSVCVPVAAHF
                     SAVIGCSPVPFTVTFTNLTSILPGETISGYFWDFDGTNTNTSTAVNPVYSYASAGNYT
                     VTLTVTLMGGCQATITQNVMVTGLGAPSISINPNPPCAGQPVQFMGMATGSVISWLWN
                     FGNGASNGSQSPMQTYLMDGPYTVTLTVMDAQGCTNSSTQNIVVQPTPSGVITPSTTA
                     PVCAGQSVTLTAPAGTGYTYLWSDNSTGPTLTVTSSGTYSVQVTLGPCSYTTPAVTVT
                     VLPPPPTVISGNLVICDMGCTTLLAPPGYSYQWLDPVNPILGQTGASLQVCSGSLSPA
                     YAVSVTDGNGCSAVSPPVTVTVAVSPSFVVNAVPTPACEGTPSVLTISPVQANVVYSW
                     SNGGSGTSITVLQAGSYTATGVDTLTGCSSSAFAIVNPLPDLCLVPTGCYKACNPDTI
                     CGPAGLASYQWNFNGAPITGANSPCLVVTQSGSYSLTGTTAAGCSATSDTLILELMDC
                     GCENLFDVTTTPVSGDTCCWTLSYNNSYTGPISGLNIHTQDADFDFDLSSLHPSLTPV
                     GFSPGNLFLTGASWSNMPQGALNNFIKFCLKNVSNSPQQIIFDWYDDVYDIVCSDTLE
                     LECPLEPDCLYLLSDSIYCENKEVKYTFTVCNPIDAAFSVGYILLQPVSPNGVTITPP
                     AINASPAIAPGECRTYTVMLGGGTSIFGDTLCFRMLAHDFDPATVDTALCCSLDTLYC
                     IAIPDCEPCDDIEVAARPIGPTGTGPCCFNVTLINNFSGNYFDGVGLCLITPGATFST
                     FANTLGSGWATIGYSPTVIMLDVIPPLGSALPLGATNLPVLCINTAQFANPLLEIKWM
                     QGDSVVCRDTVVLACFPPCGYFSEEEVVCSPNGGWLYQGNFNNTSPYTINQLNMVFTS
                     PPGLGAYNQSLPGTPVPPGGQLAFSIPIGAPAMAGDSVCFTMAMHDTLNVECCNVRHC
                     IELPECASPCIDSTQIDLTLPCLAIYDPVCGCDNVTYSNACVAQNHFGVTSWTSGACP
                     MDQIR"
     assembly_gap    39756..39838
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39839..40355
                     /locus_tag="PK198_00955"
     CDS             <39839..40355
                     /locus_tag="PK198_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37329.1"
                     /translation="FTYRFTPPADLLVCDGVRWRFRRFPITVASPWTNMGTTSANAPL
                     TYTFAGTGIYQVCMEVQRTTPNGEVCSREVCVFVNVFAQLRDDRGEMLSIAPNPFNGE
                     LFVSSPAPESMQMDVLVRDVLGKVLLRFRKDELRAGEAWPLDLTGLDAGVYLLECTGG
                     NRKQVLRVVKQ"
     gene            complement(40873..41709)
                     /gene="hypB"
                     /locus_tag="PK198_00960"
     CDS             complement(40873..41709)
                     /gene="hypB"
                     /locus_tag="PK198_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRF37330.1"
                     /translation="MCTTCGCNQPEEAVTIHTHAEGHAHEHHEHGHEHHHHHGHEHGH
                     DHHHHGEKTVIELEQDILQKNNLLAERNRGYFDAKNILALNLVSSPGSGKTALLERTI
                     RDIGTEMPVFVIEGDQQTMNDADRIAATGAPVVQINTGKGCHLDSDMINKALKKLNPA
                     EGAVVMIENVGNLVCPAMFDLGETARVVIMSVTEGDDKPIKYPDMFHSAHLCIINKTD
                     LLPYVPFDVEKAKAYARQVNHHLSFIEVSAYTGEGMEAWYKWLRHALHHARRGEAVHH
                     HH"
BASE COUNT         9384 a        10892 c        10880 g         9612 t
ORIGIN      
        1 gggcttcaca tacatcggat tggcctcatc accgttgaca atgattttta cgtcgccctg
       61 cggcaccaat ttcttgcgcg catagatgag catgaaggcc aagcccagaa taactccgcc
      121 aaacacaacg atggcccaga gaatattgat tggattgaat aacagtatca taattgagat
      181 acgattagtt gtgaattatt tgccggcggc catcaattct gcgggattga tacccatcag
      241 gctcatgaat gccatgccca tcaggccggt cagaataaaa gccataccca aaccgcgcaa
      301 tgccggcggc acatgggaat agcggatttt ttcgcgaatg gcagccaatg caacaatggc
      361 aaggaaccag ccaaaaccgc cccctatgcc aaatacagta gattctgcca aggtatattc
      421 acgagccacc ataaagagcg cgccgccaag aatagcgcag ttcacggtaa tcagcggcag
      481 aaagataccc aatgcgttat acaaagaggg agacaccttt tctaccacca tttcaactaa
      541 ctgcaccatg gcagcaatag tggaaatgaa caaaatgaag cgcaggaaag tgagatcggt
      601 gccaaaaatg ccccgctcgg tgagcaggta ctcattgagc agccagttga tgggcatggt
      661 aacacccaat acaaagatga ccgccaaacc cagacccaca gccgttttta ctgttttgga
      721 aactgccaaa aaagagcaca tgcccaaaaa gtaggcaagt accatgtttt caatgaaggc
      781 agatttgagg aagatattga agagttccat ttcggaatgt tttaaattat agtcattttg
      841 caagttacgt tcatcttcag gatggacata agatcaggat acatctacca ggctcttgtt
      901 ccaggagcgc tgaatccaga tcaaaatacc caccaaaaac aacgcagccg ccggcaatac
      961 catcaggccg ttgttggtgt aaccgtagag ccaactattc ggatcggcgg tattgatcca
     1021 gtaatgagtc agttcattgc tcggcccaat gatgttcagc gcaataggcg tacctgtaaa
     1081 gagcgtgccc ttgccgaaca actcgcggaa aaaggcgaca atgaggagga tagctccgta
     1141 gcccaggcca ttgccgatgc cgtcgaggaa agacttccag ggcttgttgg ccattgcata
     1201 agcctccaga cgccccatca cgatacagtt ggtgatgatg agaccgatga ataccgacaa
     1261 ctgcttccac accgggtagc tgaatgcttt gagagccaat tctacaatgg taaccaacgt
     1321 tgcgatgata accaactgca caatcatgcg cacctgcttg ggaatgtaat tgcgcagcgc
     1381 agaaaccgtg acattagagg cggccgttac aaaagtaacc gctatggcca tcactaaagc
     1441 cggcaaaatc tgcgtggtga ccgccaatgc cgagcagata cccaacacct gtacagtgat
     1501 cgggttattg ccttccaggg ggtctgtcag caatttgcgt tcttttttgc cgaaccaggg
     1561 ttctttttcc tgaacggaca tcgttttggt tgcggtatca gccatgactg ctgttttttt
     1621 aggttggatt cttaatgaat tggaattgcg atgaaagtta ttgtttggcg gcagcctgcg
     1681 ttttttgaat gtacggctcg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnggga aagcagggct gtctttgatc tcggcgccca aaccgggtgt ttcgcctttg
     1921 tgatcaaacg agacgccggc gatggtattc atgtctgaat caagcgctat gctgccccaa
     1981 atctcgtccc acaagccatt gccacgaaca gaaagaatgt aatagggctt gccctgagcg
     2041 tctttgtaaa caaacaccgg cagcaaacga tctgcttccg gctttttctt ttcctgagcc
     2101 aggttgattt tttctgccaa ctgaccgctt acctctnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagtcatgg
     2341 ccaaaacgaa tctgagaata taccccgttg aattcacgac gcttatgttt taatttgcct
     2401 gattcggccc gcaaaccggc tcagataaaa gattattttt tcaaaattct acgccttggc
     2461 cgcagccgac ttggtacgac gcttgatatt gccctgtacc actacataat caatgagcgg
     2521 agcaaagaca ttcataaaca agatggccaa catccagcct tcgggatagg cggggttcaa
     2581 tacccgtacg atcagaccta ttaccccgat caggaagcca tacacccatt tgcccacatt
     2641 ggtactcgaa gccgtcacag ggtcggtagc cataaatgcc atagcaaaca ggaagctgcc
     2701 catagagaag tggtaatacc agggcacttc catgaacggc gtagcgcccc aggcattgaa
     2761 cagcaaaccg gtgaacgcca aacccaacac catagaaacc atgatgcgcc aggatgcaat
     2821 accggtggca atgagcataa gcgcgccgat aatgatgaac ggcttgttgg tttcgcccac
     2881 cgaaccgggg atcaaacccc agaacatatc agacgggtta taaacctttg tgacagcctc
     2941 ccagccgccc ttggcagcca gcgccatggg cgtagcgcct gtgtaaccat ctatcacggc
     3001 agcaccgccg gccccgaagg tttcccaacc caagcctttg aaaataccgt cgaacaaatg
     3061 atgcgcccaa ctgtacatct cacctgcata agcgccgttt actttctcaa ggccggaaat
     3121 ccagacttca tcgcctgaaa tatggccggg ataggcaaag aacacaaata cacgcgagag
     3181 caatgcaacg ttgaggatgt tcatgcctgt acctccaaag gcctctttgc cgatgataac
     3241 cgcaaaagcg accgacaatg ccagaatgga caaggggaga tcgggcggca tgatgagcgg
     3301 aatcaaagct ccggacacca gaaaaccttc ctcaatgccg tggccttttt tgtacgcata
     3361 gtagaactca atacccaaac ctaccacatg cgccacgatg atgatgggca gcagtttgat
     3421 gaggccgtgt accaatttca ggtgccaacc atcccataat cccacatact gacccaatgc
     3481 ggcaaagtgc tgatgcccgg tattgtacat cccgaacaga taacacaatt gcatagccag
     3541 caccacgaat accatggtgc gcttgaggtc catgccgtcg cggatatgag cgccggtttt
     3601 ggtcacatgg tcgggagtga gcgcgaaagt aaagaagccg tcatacagcg tatggaggaa
     3661 gggcgacttt ttcttgtcag gttcaatgct gctgaaaaat ttgagcagtg cctttttcat
     3721 attgttcttg ttgtcgttgg tcgttgttga caaaaaaagg aaaacagtgc gttgaattac
     3781 atttgctcct gcatcatttc caaaccgtct tgcagtatct gctggagcga ctgtttggag
     3841 gtgcaggcaa attcgcaaag ggcaacatct tcctcactca attcgagaat gcccagacct
     3901 tccatctgtt caatatctcc ggcgagaatg gctttgaaca ggtgctgagg caaaacatcc
     3961 atcggcagca ccgattcgta ttggccggtg accacaaagg cgcgcttttc gccatgcgta
     4021 ttggtattga catggaacga acggcccgga gctaccagat tgcccagata agtgcgggaa
     4081 accgtcgggc gggcgtagga aggagccagc cagccgaaca tttcgaagtc gttgccttct
     4141 tcgatgatgg tgatttggtc gtcgaaagga ctcaggaagc cggctgcgtt ggtcgtggcg
     4201 ccggagagaa tgtcgccgga aatgatgcgc acgttttcgc ctttgatatt gccggtcgtc
     4261 aggtcgccga tattggcgcc gatgtgtgtg gacacatagc cggtcgtctt gcactcgtcg
     4321 ccgctgagcg ccaccaaacg ggctgcgttg aagcgctttt cggtgaacaa tgccccgatg
     4381 gtgacgacat cctgcacgtt cagcgtccac actttttcgc ccgatttaat gggattgatg
     4441 tggtggattt gaacgcccac gttgccggcg gggtgtttgc ctttgaagct cacttttttg
     4501 acgcctttag cttgcgcaaa tacagccgat ggcgccgatt ttccgcgtcc atccaaaccg
     4561 agccatacgc tgccgtcggt caggcgggcc agaacatcca agcctttttg gaaggcttcg
     4621 cccttacctt ctactacatg gttgagatcg ggcgccagag gagccgtatc gaaagtagag
     4681 atgaatatgt cgcggggaac atcgcccgga gccggaacta tctggaacgg acgttggcga
     4741 atcaatgccc aggcgccgct gtccatcagg aagtcctgaa gctcctggcg cgaacaggaa
     4801 ttgagttgtg gcggagtgag ggcgcggtgc tgctgctctt tgtcggccag aatgaccact
     4861 tcggtgatgg cgcgtttggc gccgcgattg atggcaatta cttcaccgct gaccggggcc
     4921 acaaatttga cggagggggc agatttggca tgaaacagcg catcgcctgc ttttacttcg
     4981 gctcccactt ccacttccat tttgggaatc gggctgaggc ctttgaaaac gggcgggcaa
     5041 acagcgtaag tttttgcctg tgcggcaacc tgcgttttag cgggtacacc ttttaatata
     5101 atatcgtggc cctgttgcag cgacaccgtc gagccgtctt gcgcgccgcc ggcacttttt
     5161 ttgccggcga acttgtcgga tacctgcatc cagatacctg tgagcaacaa tgccccgatc
     5221 gcatacaagg catacatgga tttgctgccc gaagagcccg cgctttgggc cagaatgtcg
     5281 cccgatgcaa tgccccaggt tacgagcaaa agaaggaact ttaaagttgt gtttttcatc
     5341 agcttcgtct ttgtaacgga agggtatcaa ctcttgaaaa catacccttt gaaaaaagca
     5401 gcgcaaagtt agtaaccttt gcattatcta aaagtagaat tattttttag tgttcagaca
     5461 ttgatattat ttagacacaa tctacgcaga atggttttca tgcagcatgt attttcgcat
     5521 ctgccagaaa ttgtaccgta gcctgcatgt catcgctgag cacccggtct tcgcgcaggg
     5581 gcggcaccaa ttttctgtaa gcctcaactt gtttttccaa tgccggagag ctttgtgccg
     5641 gctgccgcag atacagcgcc tgcgcggcag tcatccactc tatggcgagg atagattgta
     5701 cattgttcag tacccgccac gctttggtag ctgcattggc cgccatactc acatggtctt
     5761 cctgcccgtt gctcgacaca atggagtcgg cggaggcggg cgtacatagt tgcttgttct
     5821 ggctggcaat ggaggccgcc gtgtattgcg gtatcatcaa cccggaatgg aggccgggca
     5881 agggggtaag aaaaggcggc agcccacgcg cgccggagat gagctggtaa gtgcgccgct
     5941 cggaaatggc gcccagctcg ctgacggcaa tggccaagta gtccaacgcc agggccatgg
     6001 gctgcgcatg aaaattgccc cccgacagaa tttcgtcggc atcggggaag atcatgggat
     6061 tatcggtcac tgagttgatt tcggtttgca ccaaatgccg cacatgctct atggcttccc
     6121 aggaagcgcc atgcacctgc ggcacacaac gaaacgcgta ggggtcttgc acatgcgcct
     6181 tctcctgtgc agctatttcg ctgccggcaa gccaatgtcg aatggcggct gcggtgcgta
     6241 tctggccgtc ataagcgcgg acgatgtgca atttttgatc atagggcgac aagcggcact
     6301 gaaaagcatc tgccgacaag gccgcgcaca tgttggccgt agcaaacaat tgctgcgctt
     6361 tgttgactgc ccacagcgca taggccgtag aaaactgggt gccgttgagc agtgccagac
     6421 cttctttcga ttggagccga agcggcgcca tacccagttc tgccagcact tcctctgccg
     6481 gtcttttgcg gccctgatac catacctcgc ccaggccaat aagcggcagg ctgagatgtg
     6541 ccagcggcgc cagatccccc gaagcgccca gcgacccctg ttggtaaacc accggtataa
     6601 catcggcatt gtacatgtca atgaggcgct gcaccaattc gaggcgcacc ccggagtggc
     6661 cgtaagccat gttcctgatt ttgagaaaaa gcatggcccg cacaatctcc acaggcgcct
     6721 cctcgcccat gccacaggcg tgcgatttga ccaagttgtg ctggagttgc tctgtttcat
     6781 cgtcggagat acggatattg cacagcgacc cgaaacctgt gttgatgccg tagagcaact
     6841 ggtcggaatc ggccatgcga cgctctaagt atttgcggca ggcgatgatg ctttgggtag
     6901 cctctgttgc cagttccagc ggcaggcggg attcaatcca gtgttgcgcc tgttccgggc
     6961 tgatgcgttc gtgactgatg cggtgcatga tgttgtgttt ggtataaatg gtcaggaaat
     7021 cggggggcaa aagtacgcca tatagttaag aacatctcaa gtccgttaaa ccttagttaa
     7081 accgggggct gcgcagtgac ccagaaggcg tgcgcggcgt ttaaagcact aaatgcaacg
     7141 cacacaacac aaaacaatgc ttagcgtttg tacctttgac ctaattttca ctcaaaaaag
     7201 agaagcatca tgacttttta cagcctgttc cgcaacggaa aaattttagc cctcgcctta
     7261 ggttttactc tggctttttc taccgccgcc tccgctcagc gcattgcgta tgtggacgtg
     7321 aaccgcattc tggagagttt caaagaatat caggatgcac aaagtgaatt ggatcgcgtg
     7381 gcctccaaat ggcgtcagga aatcgctcag gaatacgatg tcatcaaggg cctctacaat
     7441 cgctatcagg ccgagcaggt actcctcagc gaggatgccc gccgcaagcg cgaagacgaa
     7501 atcatgaacc gcgagaaaga agtgcgcgac ctccaaaaag cgcgtttcgg cccggaaggc
     7561 gagttgttcc gccgccgcca ggaaatggtg cgccccatac aggaacgggt gtatcgagcc
     7621 attgaaagct atgccactga gcgcggctac gacttcattt tcgacaagtc gggggctgcc
     7681 ggcatcatct ttttcagccc ggcttatgat aaaaccgacg acgttttgag tcgtctcaag
     7741 taatttcgca ttacctttgc attcattttt ttattcacca aatctcaaat aaacgtacac
     7801 tgagatgatg aagcatttgc tcaaaacagg attggccctc ctgctgatgt tcggcctgac
     7861 acaaaccatg tcggctcaga aattcggcta tatcaattcg tccatgattc tggcagaact
     7921 gcccgaagtg aaaggcgccg aagctacgct ggaagcactc caacaacagt tacagaagaa
     7981 aggccagtct atgctggaaa aactacaggc cgactacgcc gttgtgcaac agaaagtaga
     8041 acgcggcgaa ctctcgccca aacagcagga aacggaaggc gccnnnnnnn nnngctgccg
     8101 aagaagaaat cggcaaattt gagcagaata tgatggcaca attggaaaac aagcgcaacg
     8161 aactgctcaa gcccatctat gacaaggtga accaagccat caaggatgtg gccaaggaaa
     8221 acggtttcca gttcatcttc gatcagggcg tattgctttt cgcagaagaa tctcaaaatg
     8281 tagctccgct cgtaaaaagc aagctcggca tctgatttcc tctgttggaa tgcaacattc
     8341 gcccaatcaa cctattggag tttttgactc cggcattggc ggcctcacgg tcgccaatgc
     8401 tattatgaga gctttgcccc aggaagaaat catttatttc ggcgatacgg cgcacctgcc
     8461 ttacggcgaa aaatcggccg acgccatccg ttactactcc cttcgcattg ccaaattcct
     8521 gctggaacgc cattgcaaaa tggtggtcat tgcctgcaat tccgcttcca cagcggccta
     8581 ccgggtgttg ctggagttct tcaaaggaca agggcttttt gtcaatgtgg tggacccact
     8641 cgtggaatct gtggccgccc ggcgctttca aaaagtgggc ctcattgcta ccaaagccac
     8701 cgtacaatcg ggcatatatg agcaacaatt gctgcacctg caacccggct tagaggtgca
     8761 ctcattggcc acgccgctgc tggcccccat gatcgaggag ggcttcgtac acaatgacgt
     8821 cagtcaggtg gtattggaaa gctatctggg gcacgagcaa ttgcagggca tcgaggcttt
     8881 actgctggcc tgcacgcact atccgctcat caaaaccgag attgaaaatt tttaccatca
     8941 ccaggtagag atttttgact ccactgatgt cgtggcgacc gaggtaaagc gcaaactggc
     9001 agaacacaag ctcctcaacg agcagcgccg ccatgcgcac cgctttttcg tatcggacta
     9061 taccccgacg tttgaacaaa ctacccgcat tttttatcag gaagacattg tgctggagca
     9121 ctatccgatg tggtgattat ccatccagcc cgatttctat gcgcgagcct ttcgcccagt
     9181 cgttgaacgg cgcaaaaatg tgaatctggc cctcttctac tttcgcagca atgcgctcca
     9241 cgtccacgat gggcagcagc ggcaaattga ctaagtaatg cggcagttca acctctccgc
     9301 tgagcacctc catcatgtaa tagacgacga accgccggtt gacggcctca atgcggattt
     9361 tttccaaggg gatgcccggc gtgcgtagct caaggtgaaa gccggctttg tcttgccatg
     9421 ctttcgcgcc gccctgtgcc atgccgcctt gtacggtgtt ggcgaggtcg tattgcgcca
     9481 tcagttgctt cgatatggat atttgcggtt tcatagctgc gtcaatttga gtgtttggaa
     9541 tacaacgctg caaaccggat gccggatcga gttttcgaca tttttggcag ttgagactga
     9601 aattttggca cttacacctc ctcccctgct atccacaccc gctccaccac atcgctgccg
     9661 aaagcatacg gtagaaaggc cagagaggaa accggcttgg tgaggaaaaa attcgccgac
     9721 tttcccaccg caatgctgcc gtgcgagtgc gatatttcca tggcagccgc gccgttgagc
     9781 gtggcggcgt tgacggcttc ttccggcgtc atgcgcaatt tgatgcaggc caaagaaacg
     9841 acaaacggca ttttgcccga cggcgaagag ccgggattga agtcggaggc cagaacgagg
     9901 ggcaaacccg cgtccaacat acggcgggcc ggcgggtaat ggtcgttgag gaaaaacggg
     9961 gccgagggca gcaaaactgg caacgtatcg ctgtttttca agcagttgat ttcttcgtcg
    10021 gtcaggactt ccagatgatc caccgatacg gcgccgcgtt ctacgcacac ctctataccg
    10081 ccgagacagt tgaactgatt ggtgtggatt ttggccttga gaccgtagcg cgccccggct
    10141 tcgatgaggc gggcagtttc ggcaggactg aagaagtttt tctcgcaaaa agcgtctatg
    10201 tagtcggcca gaccttcgtc ggcgatgcgg ggcaacattt cctcctcaat gaggcgcagg
    10261 tagccttcgc ggtccgtttt gtactcggcg ggcagggcgt gcgcaccgag gaaggtagcc
    10321 ttcacgggca ttttcgtgcg ttcgcgcaaa gcccggatga cgcgcagcat tttcaattcg
    10381 ccctctacag aaagtccgta gccgcttttg atttcgatgg ccccggtgcc atagcgctgc
    10441 acttccagca ggcgttgcca ggccaattcc agcagcactt cttcgggcgt atcgcgcagg
    10501 cgccgggccg agttgaggat gccgccgccg cgttgagcta tctgctcgta ggtcagtccg
    10561 tgtatgcggt ccacaaattc attttcccgg ctggccgcaa atacgaggtg cgtatgcgaa
    10621 tcgcaccagg taggcagcag cagtcggccc gcagcgtcca ccacctgcgc gccgggcgcg
    10681 tcggggcatt gtgtcatcgg gccaaaggag aggattttgc cgttttcgac cagcagaaaa
    10741 gcatcgtcca ctgagggcaa aacctgcata tcggcgccgg ctacgcgctc ccggagcggg
    10801 gcggtttctg tttggaaaat ggtttggatg ttgcggagga ggagcatgag gtgaggatga
    10861 atatgatgaa tttactacca aggccacatt ggcccttcaa ccagtttggg atgaacaata
    10921 atcagaagaa caagcaccaa aaccatcaat agatacggtt ttttcaagtc ctgaacttct
    10981 ctgtttttat accacacaac cgccattatc agcgaactca ttattggaac aaccatcgtc
    11041 cccagagcaa tgtagctact atacagcgca caaataatag ccctgtcaat gacatttcca
    11101 acgataaacc caagaatggc cgcattgcca aagcgttcat agccgaaaag tatcactcct
    11161 ccaaccaaca acaagagact caatcccaac gcgctcccat agtccttcca cccaagcgag
    11221 gtgaaatagt ttacaaaaaa aataccccaa aaagtaatgg acgctcaaga gtaaacccat
    11281 gaggatagac gcccctccga gcattgtctt tttacttacc ggaatttcat acattttatc
    11341 cttatctttg tacagtgcct gctacaaagg cattcattca tctcaaaaca acatcaacat
    11401 ggtttccaaa gaccacttgg cccggctggc agaaatcgtt cagcaccccg ataaatggga
    11461 agacagcacc aaatatttcc tcgatcactt cgcctccgat caggaattta tggaaatggg
    11521 gaaaagaacc cgcattgatg tcctcctcaa aatcatgggc gaagtggcca aaaaggtact
    11581 gaacaaatcc ctcgtgcgca tcgaaaaccc gcagatgtac agggtcaaat ccatgaacat
    11641 gatacacggc tcctgcacct ccgaaggctg ggtgatgatg tttgtttatt tcaccgacat
    11701 ccacaaaggc gtgttctcta ccaccaagcc tttttccggc caaaccggct tcagctcgcg
    11761 cgtcactgcc ttgcctatgc cggaattgga caatgaagaa cgcctctatg gcgaccaatg
    11821 gaacatgaac cccaactgat tacgcctcac tatcggcata cgcctctatc ggcgggcagg
    11881 tgcagatcag attccggtct ccgtaggcat tgtctatgcg gcccaccgaa gcccagaact
    11941 tgtacccctg acgcaaataa ggcagcggcc aagcggcctt ctcgcgggaa taggcatacg
    12001 gccagtcatc ggcagtaacc aaatcggcgg tgtgcggcgc gttgtgcaac acattcgatt
    12061 gcacatcagc ttcgccggag gcaatctcat ctatttcttt gcgaatttgc agcagggcat
    12121 cgcagaagcg gtccaactcg tctttgctct cgctttccgt cggttctatc ataatggtac
    12181 ccgccaccgg gaacgacagg gtgggcgcat ggaatccgta gtcaatcagg cgtttggcca
    12241 cgtcttcggc agaaaccagg tttttgaagg gacgcaaatc cacgatcagt tcatgcgcag
    12301 tgcggcccag atcgcccaca tacaggatgg agtaatcatt ttccaatctc gacttgatgt
    12361 aattggcgtt gagaatggcg taacgggtgg aatcggtgag accgtcaggt cccagcattt
    12421 tgatgtacgc gtaggaaatg agcaggatgg aggcgctgcc ccagggcgct gccgacacgg
    12481 catctatgcc ctgttcgccg ccggtaccca ctaaaggatg tgaaggcagg aagggcttga
    12541 gtttttcgtt gacgcaaatc ggccccatgc ctggtccgcc gccgccgtgc ggaatggcga
    12601 aggttttgtg cagatttagg tggcacacgt cggcgctgat gttgcccgga ctggtgaggc
    12661 ccacctgcgc attcatattg gcgccgtcca tgtacaccaa gccgccgttg tcgtggatga
    12721 ggtcgcaaat ttcgtggatg cgcaactcaa acaccccgtg cgtagaagga tacgtcacca
    12781 tgagggcggc gaggcggtcg cggtattgtt ccgctttggc gcggaggtcg tccacgtcta
    12841 tgttgccgtg atcgtcgcaa ttgaccacca ccacatcgag gccggccatg acggcgctgg
    12901 cgggattggt gccgtgcgcc gaggaaggaa tgagcgccac attgcggtgg ttttcgcctc
    12961 tggcctcatg gtacgcccgg atggtgagca gccctgcata ctcgccttgc gcgccggaat
    13021 taggttgcaa agagcaggct gcaaaaccgg tgatgtcgct gagccaggct tccaaatcgt
    13081 caaaaatccg gcgatagccc ccggcctggt ctgcgggcac aaaggggtgc aggttggcaa
    13141 attccggcca acttaccggc atcaattcgg tagctgcatt gagtttcatg gtgcaggaac
    13201 cgagcgaaat catggagagc gtcaaagata agtctctgtt ttccaaacgc ttgatataac
    13261 gcatcatttt cgactcggtg tggtaagcgc tgaacaccgg gtgcgtcaaa cagggcnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nggaacgcgc aattgagccg gctcggcaac tgagaacgca
    13381 tgcgcagcgc ctttggcttc cgcaaaaatg cgcacgatct gctccacatc cttcagcaag
    13441 gtggtttcgt ccagactgat ctgcacgccg ccggtagccg ggtagtaaaa attgacctcg
    13501 tgtttcaggg caatttcccg gagctgctcc tgcattttgc cgtctgtttc tacaaaaatg
    13561 gtatcgaaat agtaggcatt gggcaggtga taccccagtg attgcaggtt gtcggccaat
    13621 atggcggcca aagtgtgaac gcgttcggcg atggcgcgca agccctgcgg accgtgatac
    13681 gctgcgtacn nnnnnnnnnn ggcgagcagg gcctgtgctg tacagatgtt ggaagtggcc
    13741 ttttcgcggc ggatgtgctg ctcgcgggtt tgcagggcca tgcgaagtgc gcgcttgccg
    13801 tgccggtcca ccgatacgcc gatgatgcgg cccggaatga cgcgcttgaa gtcgtcgcgg
    13861 gtggcaaagt aagcagcatg aggcccgccg tagcccatcg gcaccccgaa acgctgggta
    13921 ttgcccacca cggcatcggc gccccattcg cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnntgccg ggatattgga gcaggatacc gaatgtttta
    14101 tcgttgattt ccagcttgtt gtcgggctgc accacgattt tgatgccgag gtgtttggcg
    14161 cgggttttca gcacggaaag ggtttgggga aacacctgat cggagaccaa aaactcgttg
    14221 gcgggttcgc ctttggcccg tttgtttttt tgttcgtaaa acatgtgcat ggcctcggcg
    14281 gcggcagtgc cttcatcgag cagagaggcg ttggcgatgg gcaagccggt cagttcgctc
    14341 accatcgtct ggaaattgag caaagcctcc agacggccct gagcaatttc tgcctgataa
    14401 ggcgtgtact gcgtgtacca gccggggttt tggaagatat tgcgggcaat gaccgatggc
    14461 gtgatggtgc catagtaccc catgccgatg taggttttgt aaagcttgtt ttgggcagcc
    14521 acggccttca attcctgaag gtattcgaac tccgactcgg ccggcggcac gttgagcggg
    14581 cgttgcagtc ggatgctgtc ggggatggtt tcgtagatga gttggtccaa agagtcggcc
    14641 ttcacgacgg ccagcatgtc ctgcaattcg ccttcattga tgccgatgtg gcgattggcg
    14701 aagcggtcga aaaacgatat tttgctcatg ttgtagcggt tgtatgcgtt ggttaggaaa
    14761 acgcccgaaa ggtaatgctt ttcagacagg gaggaaacaa acatacgtca agtgcgttca
    14821 aaaaaaatgg ggcgggcagg ggtgggtgag cgattggccg cagcggcagt atgactaaac
    14881 ctttccgaag ccggggaaca aagacgccca tcattcgcaa ttcgcgaatc gcgaatggag
    14941 gtattgattg attttgtgtt ttgtgtactg cggtgaaaag ctatccagac aattgaaatc
    15001 catctttcgg cttcaaaccc tcggaagggg ccaggcaaga tttgacaaat ttttgaaatt
    15061 tgtcaaatct caggcagcaa atctcaggtg gggcgccggg caatgccatc tgttcatacc
    15121 tttccatttt atccaattga tcgcggaagc ggagcttcaa atcggaaagg ttttcgtaag
    15181 aagtgtaaaa atgctccagt gccctgagtt tttctttgaa atccaacagt gtttttacct
    15241 cgttggtaat gctgcccatt ttgatatcac catttttgaa aaaggtaaaa atttgcggct
    15301 gccgtccgcc cgactcctga aactgtcggt gcgccacgtc aaattcctcc tctgtgaact
    15361 tcccggtttt ggtaaaaaac aagcgcctcc cactggctgc tcaaaatcaa cgtctccaac
    15421 cactccatcc ccgacaggtc gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaatgag
    15541 ttgttgagcg agttccgaca tggcgtttgt ttgaggtgtg caagataggg agttttttta
    15601 gtagtataga ccttgcaagg tttggcaaac ctttcaaggt ctgtacagct acgcgtgctt
    15661 agctttattc cccgcaacga gttattgagc gcgttccgac atggctggtg tattcatttt
    15721 gggtataaaa tccaattttt tcccaatcag gattttccct cgtccacctc tttcagtctt
    15781 cgctcaaaat cttccnnnnn nnnnnnnngg atgcttgatt tgagattatc gggcaaagcg
    15841 tcagcgagca gatacgatgc aacaccgatg ggtttattga ggtcgcgaag ggcgtattca
    15901 actactacat tctttttgtc tttgcaaaga atgataccta tggaagggtt gtctatcggc
    15961 gacttcatcg aagcgtccaa tgcagacagg taaaaattca tttgcccggc atcggccggc
    16021 ttgaattttc ccatttttaa atcaatcgcc acataacacc ttagtttcag atgataaaaa
    16081 agaaggtcta ttttataagt atcatcttca atggatatgg gaaattggtt gccaatgaat
    16141 gcaaaacccg aacctaactc cagcaaaaag cgagtgatat gcttcatcaa taatgtttca
    16201 aggtctttct cacgagcttc tttggccagc gtcagaaagt cgaaaatgta ggggtctttc
    16261 aaggtttgct gtgccatgtc ggaatcgggg cgaggtagcg tttgttgaaa attgctgggc
    16321 gcaccaccct gtctttcgta taacttgctt tcaatttgat gcaccaacac atcgcgcgac
    16381 caaccgtttg cgagtgctgc ggcggcatac caaaaacgct tgatttcgtc tttgcatttt
    16441 tggagcagcg ttatgttgtg gtaccagcta atttgtgcaa gtgacgcttg cacaaattca
    16501 aaatcgggat aagccaatgc aaattgccgc atataaagca agtttctttt agacagacct
    16561 tccatttcag gatattctac acgaaggtct tcgcccaatt gatcaacgac tttgcttccc
    16621 caaccgtgct gttgttgcct ttccaaaata ctcttgccga tatcccaata caaaaacaac
    16681 atttcgcgat tggctgatac catcgcgcga atctgtgccg tgcggatttt acctttgatt
    16741 tccgacaaaa gttgttggta cgaaaggtct gcactgtatg gctgggcatt gttctccata
    16801 aaattatact tgaattttga gatgagtctc caaccactcc atccccgaca ggtcgggcag
    16861 ttcggtcagg ccgcagcggc cgaggtacaa ataagaggca tcctccccgc gggcgtgttt
    16921 ggctttattt tcggcaatga gttgttgagc gagttccgac atggcgtttg tttgaggtgt
    16981 gcaaggtaga gagtttttgg ggttttcagc gcgtgctcag gtcttataaa gtttgcaaaa
    17041 ccctatctgt ttagcggaag ttttcaggtg gcatctaact gccgagcttc ggcgaggcgg
    17101 gaaggtgtcg catgaaaatg ggggagattt ctccttccaa agccccgctg ccgcgagcgt
    17161 gcagattaag acagcggtgg tgatgagtag aataactgtt caaaatcgct gagggtgact
    17221 attcgggttt gctcaaattg aatgagggtt aaaaggtcat tatcgcccgt cagcaggtaa
    17281 tcagctttgc catcgtgaca tatagccaac aaataatcat catcgggatc tctgcatatt
    17341 tgaaactggc tttcagtttc aataaacgtc agtcggtcct ttaaaatctg cacaaatgct
    17401 gccgtcagtt ccgagcgggc tattttggaa aatttcggtc gagaaaacac atcggccaat
    17461 tctgccaaca attctgagtc ggctaaaatg acaatatttt catccgagac aagcctctga
    17521 attctctccc gcatagaaga actgatgaac gaagctaccc ataggttgga gtctatcaag
    17581 atggtcatag ggcttattgc gactgctttc tggcttttcg tacagacttc agttcggcca
    17641 caatttcgtc catgctgatg tcgggcatat cgggcaactg cggggaaagg agccgccatt
    17701 tttctccggc agccgctgcg cggagctttt cagccacctg aagcttttca gatgccggta
    17761 attccatcag ggcatccatg atttgctcca gagaaatatc annnnnnnnn nnnnnnnnnn
    17821 nnnngccggc acttacggtc attgtctcaa ttttttgtaa agatactata catatcgttg
    17881 cgaggcaagg ctattgcctc cattttttgc ccaatccgcc aattcagggg gagatttttc
    17941 cttcccgccc gcgaaacttc gaagacacca cctgaacacc ccgccgctgt acatactcct
    18001 acaccaccac cacaatcaaa tccatattga tgcggatggt attgttcacc ttcaccaagc
    18061 cgagcatggc ggtgggaggc tccatgccga aatcggtcat cagcacctct ttggaggtag
    18121 tgagccggat gcggtcggga gcgatgcgtt tggcttttac atcaaaaaca acggattggg
    18181 tgacgccggc aatggtgagt tgagaagaag ccttgagggg cgaccagtct ttgccggtcg
    18241 caagctcggc ttcctgcaat tgggcgcggg tcagttctat acgaatgccg gggtgttggt
    18301 cggcacgcag ggttttgtac atgtctttgt tcatttgcgg attgccgcaa tccaacgact
    18361 tggaacggag gtggaggctg gcatgggaga agcggagtgt tttgccgccg tcttgcaact
    18421 caaagcggat ggagcctttg gaaaactgct cagagcaatt gcaggtaaac ttattgacat
    18481 tgctggagcc ggcaatgctg aggctgctgc ccgcatctat gtaataggag gtttcggcgg
    18541 tttcgaggcc tgccatgaag gccacaaaag aagagagcaa aacgattttg aaaaaagcca
    18601 tgatatgaat gtttgttata gtttgtacag acaaaacatc aataaggcaa ggcgtaagtt
    18661 caatcgtgag atgggaaaaa caggttcagg cgaaagccct gaggtcggaa acaaccccag
    18721 ggctttgcag gttttattcg gatcagacat ttctcattag aaaccgatgg cagcctggat
    18781 aacgaagccg ttgaatttgc cttcgttgcg gtagtcggta gtcgggaaat ctttgtagtt
    18841 ctggttcaca acctctgctt tcaacagcag gtttttgctc gggaaccagc cggcagcaaa
    18901 cgccagacgg tcaatgctga tgtcatcggt aaagccttgc ggacgaccgg aagcctgcac
    18961 ataacgcgca cctacaaaca agctctcatt gggcaggaag cgatacacgc cttccactgc
    19021 caattgtgtg aacttgcgct tggcatctaa gtcttcagca taagttctgc cctgtgcaat
    19081 ctcgtaagaa ccaaacactt ccaagccttt gaacttgatg aacgggttga tcatcacggt
    19141 attgatttgg ttggtaaggc ccggattgaa acgaccggag gtgaactgag ccgtagcagt
    19201 agtagctgcg ccattagtca gtgccgggtc catcatcagg aagtactgcg aaccggtgcg
    19261 gtcacctgca tagagcgtat tgcgctggat gctggggttg ttgtacatag aagcagacag
    19321 gcggaaacgg aagtcgttgt cgaaagtctt gtcgtaagca agtttgaaga acacagacgg
    19381 cgttttcttg gtcacttctt tgttggtacc ttgcggagta gaggtgtatt tttcaatgtt
    19441 gccattgata aaaccggaac tcatacccac catacccatg aaaccgccgg gcaggaacgc
    19501 atacacttca ccgccgattt cggtagcaaa cgcatcaagt atgtagtttt ctacgaatgg
    19561 gttgaacatg gtgttgccac cgtcggagcg gcggaactgc atgtcgccga agttgggctg
    19621 gaagtggccg atcttcacgc ggaagtagtc ggagaaccac tgcggattgc cgaacatagg
    19681 cagtttgtct atctgaatgt agccgccttt tacccaaaat tcgttgtggt gacgagccga
    19741 catgtagttt tccaaacaaa cccggatacc gtcttcaatc tggaagtcga agttgaggtt
    19801 ggccatggct aagttgaaac cctgaaggtt aaccgaaagg ctgtcttcgg cttttatcac
    19861 gccatagagc aggttgcggt tggtaggatt ggtagtagat acaggcacat aagtagggtt
    19921 gttttcgtgt gtgagcgact ggaagttttg agtaaaactg ccgccaatgc ggattttgaa
    19981 tccttcataa ggcgtgtcgt cggctttggt gggttcaaaa acgttgatgg cgtctttgtc
    20041 ccatccacgg aagtattgca gtttcggttg ttgcgcggtg gctttaaagc tgatggccag
    20101 aacaaaaaga gccactaaca ggcgaagtgt actcgctttc atgattttga ttttaacagg
    20161 tgacaatcga tcagcatact gaaatgatag ttacnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtatgatt ttaatcatct gcgaaatagg
    20281 gccggaatga cattacaggc gaatgccgag cgcagggcgg cataccaagc atccgaagtt
    20341 aaagataaga aaaacaggag tttagaaata aatctactaa tctttgggct tgttttgaaa
    20401 acctattttg tacaataccc agccgaaacc ggccagcaga tgcaacaaaa cgatgatgaa
    20461 aaatacattg gccatgacga ttgattttta aaagagcaaa acagattcaa ctacatacct
    20521 cccctgaaga ctcgccttta ctcccgcagc aagctcccga atcagtatga gcggtttctt
    20581 gatggctatg acaaccacag ccgccggact ccatgccgta tatggacttg tgtggcggtg
    20641 ggacatactt cccggatggt cattacttgg ctcatgattc cttttttaaa aacttcagcg
    20701 atacgtcgct agtgtttatg aaagcacaaa tatcaccgta agtggtttct cctaatatga
    20761 tcttcatcac tcaagggctg aaacagcctg tgtaccttag ctacataaaa aatttcgtct
    20821 atgacatcct tcgacaacct cctgctcatc agcggcgcag gccggaaaac attcaaaacc
    20881 actttcgcct gcgaggtcat ccgcctccat cgaaacaagg gcgtggtggg cgtcaaaata
    20941 tcttcgcact ttcacccaca agagggcgct gtgcactgga aagccgtacc cggccaatac
    21001 gaaatatacg aagaaagccg ccccgacggc cccagagaca gccatcggat gctgcatgcc
    21061 ggcgcagaaa aggtctatta cattcaatgc aggaaggagg cgctggccga agcattccaa
    21121 tccgtactcg ccgagttttc gcccggccaa cccatcgtgt gcgaatcggg cggactggcg
    21181 gatgtactgc gaccggccct gcacttagct ctgaaaggag acggaaccaa agcattgcac
    21241 ccgctggccg atgtgatgat tgaaaaagat gaaacggggt tttccgtatc tccggagcgc
    21301 atcgtttttg aaaatgggaa atggggaatt gcagcatgaa tggggtctct gtaaaaaaaa
    21361 cagtcgccgt cacgcaacag atcattgcgc aacagcgacc attcattcat aatcacaaaa
    21421 tatttactga acagccgcgc tgctcatcac caccgaaaac ttgacggtga cttctttgcc
    21481 taccttcacg gtacccatca gggcggtggg ctgttccata ccaaaatcag aaagaacaat
    21541 cagtttggat ccgctgaatt ccagatcgcc attggccaat atttttcctt tggcttccat
    21601 cggcacactt ttagtgacac ccgcaatgct gaggttaccg gtcgttttga tcacaaactc
    21661 ggcgccgctt ttgctgatgg attctacttt ggagagtttg aaagtgattt tgggatgctg
    21721 atcggccttg agggcttccc atgttttggt atccatcact ttgcctttgg tgctgatgat
    21781 ggatttcgcg tccacttcca cgctcaggtt tttgatgctt tgtatggcgt tcacatcggc
    21841 cataatatcg cctttggccg tcactttatt gactttcgat tcccattcgt gcagtgtgga
    21901 tgttcctgct acggtcattt tgaagctctt gacctgataa gcggattgtg caaaaagaaa
    21961 catgctcgtc gtgagcataa agattccaga aatgatcagt tttttcatgg caataaatta
    22021 gttgttgatg ccacaaatgt acttacatgc gaaaccggca cacgatgact tttctatcga
    22081 ataggtatga tttatgtcac tctcacccct cactcactca cccctcactc actcactcac
    22141 tcactcactc actcactcac tcactcaccc actcacccac tcacccgtgc cacactcacc
    22201 cctgccctac tcacccagcc caaccctctc tgtccaggct cctgaaatgt atggcttccg
    22261 ccagatgttc gattttgata tcggggctgt cggccaaatc ggcagcagtg cgggccactt
    22321 tgaggatgcg gtcgaaggcg cgcgcgctga gttggagttt ttccatggct ttttttnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnncacatcg gtcctgtctt tgaaccgttc ggcctgaatt tcgcgggcgc
    22501 ggatgacccg ctgcacaatg tcggcgctgg tttcggaggg gcgctcgcgg gaggctaatt
    22561 cgtcatagct cacaggcgtt acttccacat gcaggtctat gcggtcgagc agcgggccgg
    22621 agattttgct caggtagcgc ttcaccacgc cggggccgca cacacattct ttttcggggt
    22681 ggttgtaata cccgcaggga cagggattca tggaagcgat gagcataaat ccggcggggt
    22741 agtccaccga ggttttggct cttgaaatgg tcacgcggcg ctcctccatc ggctgccgca
    22801 agacctccag cacactgcgt ttgaactccg gcaattcatc caaaaacaac acgccgttgt
    22861 gtgccagcga gatttcgccg ggcatgggat tggagccgcc tcctaccaat gccacgtcgc
    22921 tgatggtgtg gtgcggcgcc ctgaatggcc gcgtggtgat gagggccgcg ttggagggca
    22981 acatgccggc tacggagtgt attttggtgg tttccaatgc ttcgtgcaac gacaggggcg
    23041 gtatgatggt gggcaatcgg cgggccagca tggttttgcc ggcgcccggc ggaccgatga
    23101 gaatggcgtt gtggcccccg gcggcggcca gttcgagcgc gcgtttgatg ttttcttggc
    23161 ctttgacgtc ggagaaatcc acctcgtagt tgctcacgtt gttgaaaaac tcctcgcggg
    23221 tgttcaccac cacaggttgc aggggcgcgt cgccattgag caacccgacc gcctcgctca
    23281 gattttccac gccatacact tccagatcgc tgaccaaagc ggcctcgcgg gcattttgtt
    23341 tgggcaaaat gatgcctttg aatttttctt ttcgcgcctg aatggcaatg ggcagcgctc
    23401 ctttgatggg ccgcaaaccg ccgtcgagcg acaactcgcc catgatgagg tagcgctcca
    23461 gatcgtcgca aaccaattgg tcggtggcgg ccatgtagcc gatggcaatg ggcaggtcgt
    23521 aggccgagcc ttctttgcgt atgtcggcgg gcgccagatt gacgnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnt ttaaaaccgg agtttttcaa cgcggcctcc atgcgctgcg agccttcccg
    23641 cacggcgctg tcgggcagtc ctaccataaa ataaaactgg ctgcccacag ccacattgcc
    23701 gccggtattg atctcgacgg taatcgtttg ggcatctacg ccatgcacgg cggcggcaaa
    23761 ggttttgaca agcataatgt gagagtttag agcggccgga ttccaacggg cctcctaccc
    23821 gccgaaaacc ggctattatt ttcatgatca gggtttccaa acgggcaata caatggaaga
    23881 cgcatgcttc gcatcgtgga aaatgcggat ggtggatttt ttgaaatcgc tgtcgtcaca
    23941 ggtgtaaata ttgacaaatt gctgcggatt gcggtccacc aaaggaaacc acgagctttg
    24001 tatttgtatc atcagccggt ggcctttgcg gaaagtgtgg gctacatcgg gcagctcgaa
    24061 gcgcacggtt tctaccttgt tgggcgtgaa tgcctccggt ttttcaaaac tgttgcggta
    24121 gcggccgcgc atcacttcgc cgcgcaccag catttggaag gcgcccatcg ggtactcgct
    24181 gccttcctcg tagcggaagt cgtcggggaa aacgtcaatc actttcacca caaaatctgc
    24241 gtcggtggtg gaaatggcgg ctttgatgtc ggcaatgacg gtaccggcca gagtaaggtc
    24301 ttcggccaac acaggcgttt caaacaccaa tacatcggtg cggcgggctg caaatcgctg
    24361 atcgtccagc atgtactcgc gggtgcggcg caggtgtacg ccctcggcat acggcaccgg
    24421 cttggcgggg tcgctgatgt attcggtgaa ggagttcgct gcggcaggtt tgttccaatt
    24481 cagttttccg tcggcttgca ggtacatcgg gcggtcggtt ttggcgacag gcggccattg
    24541 cggcaggtgt tcccagcgat tggcgccggt gaaaaaaatg gtggcttcgg ccaatttttc
    24601 gaggctgccc tttcctttga ggtggtagtt gaaaaacggc atctcaatgt gattgaggta
    24661 ccagtgggcc gtgttggaac cgaaacggat atggccgagt tggtcgcccg gctgccttgc
    24721 ccattggccg tgcgcccagg gtcccatcac gatgcggttg tcggtgcggg gacttttttg
    24781 ctcaatggct tcgtacaaac gccaggcgcc gtagcagtct tcggcatcga aaaggccgcc
    24841 taccacgagc atggccggct gtacgttcgt gacgtggttg cgcgggttgc gggctttcca
    24901 ccagtcgtcc aagttagggt gttccatcag atcgttccaa aaggcaatgg aatcacctaa
    24961 gagcttagtt ttgaaatact tgagcgcacc ggtttccaaa taaaacttat agttgtcttc
    25021 caccgaaaaa gggaatccct ccgggcgagc agtcgtcggc ttgggtcttg gcttgccgaa
    25081 accggagtaa aacgagaagc cgtccatgag gaaaaacgcg ccgttgtggt gaaagtcgtc
    25141 gcccataaac cagtcggtca caggcgcttg cgggctgacc gccttgaggg ccgggtgcgc
    25201 gctgagggcc gccatggtag aatagaagcc gggataagaa attccgaaag cccctaccct
    25261 gccgttgttg ttgggcacat tggccagcaa ccattcgatg gcgtcgtagg tatcggaggc
    25321 ttcgtcggtg tcttgcggcg tttttttatt gggattgaaa gggcgcacgt ccacaaactc
    25381 gccctcgctc atccagcggc cgcgcacatc ctgaatgacg atgacgtagc cctcgcggag
    25441 gtaatatttc caatgcgagt tccacatatc gcgccaattg tcttcgccgt aaggggcgca
    25501 ggagtacggc gtgcggttca tcaaaatggg gtgcttttcg gtagcgtctt tcggggcata
    25561 gatggtggtg aacagccgaa cgccgtcgcg catgggcaca taacgctctg atttggtgta
    25621 gttttcacga atccaggcgg agtccaaggc gttggattgc gcgaaaagcg ctgccggcac
    25681 aaggcagagg aagaagtaca aaagtcgctt catggtcgtt ggaatgagtg gtgagaacaa
    25741 tcagaatttg taggtaaaac cgaaacgcac cacaaaaggc gagcgcaaat cggtgacaac
    25801 cggcgtcacg ttgtagagca gcatgatttc ggaagcccag atacccccgc tgttgtagcc
    25861 ggcgccgatg taggtgttgt tgcgctgctg ccgttgaatg acaaggtcgt tcccggaaat
    25921 aaaagccagt gcctgttctt ccaattcgta ctctacatgg gcaaaaatgg tgcgtatgat
    25981 tttggcccgc ccgaagacgc ccgccgacca ggacagcgga ttggccgtag ctatttcgtt
    26041 gttgaagagc cgcacccgaa tgtgcgtgta gttcagggca actctggggc cgacagaaaa
    26101 catctccccg attttgtaac ctaccatggg cgaaataccc aagccgaatt gattaaaacc
    26161 attgctgccg gaaataccga ggttgacgcc gccgccatac cagagtttgt ctgtgaactt
    26221 tgaggtttcc tgagcgtaca tggcagtgcc cgacagcaaa aagagcgctg tgagcaaggg
    26281 caaaagtctg tgcgtgaagt gcatggcaaa attttttgcg ccaaagataa tgaagcccgg
    26341 cagaaaacca tgcggatctt acttcagctt ggccacaatg tcggctgcca gggtaggccc
    26401 ggcataaacg aagccggtat aaacttgcac caaatcggcg ccggcctcta ttttttcgcg
    26461 ggcagattcg gggccgtcaa tgccgcccac accaatgatg gcaaacggtg gcggcagttg
    26521 cgctctcaga tagcggacga tctgcgtaga gcgctcccgt accgggtacc cgctgaggcc
    26581 gccggcgccg atgcgctccg tcgtttccgc cggggttttg agtccggttc gcgatatggt
    26641 ggtgttggcg gcgatgatgc ctgcaatgcg cgtttcgcgc acaatggcca cgatgtcgtc
    26701 caattgcgca tcggtgaggt cgggggctat ttttaggaaa atgggttttg gatttgcctg
    26761 attatcattg cgttgttgca gctcggccag caggcgcgtg aggggttcgc ggtcttggag
    26821 cgcccggagg ttgggcgtgt tgggcgaact cacattgaca gcgaaatagt ccacccagga
    26881 aaaaagcgcc tcaaaacaag cgatgtaatc atcggcagcc tgctcgttgg gggtgtcttt
    26941 gtttttgccg atgttgccgc cgatgacaat gccggccggg cggggccgct gcaattgccg
    27001 gatgagggcc tccaagccgt cattgttgaa gcccatcctg ttgatgagcg cgcggtcggc
    27061 gggcaggcga aagaggcgcg ggcgcgggtt gccggcctgt ggccggggcg ttacggtgcc
    27121 tacttcgatg aagccgaagc ccagagccgc caatgcccgg atgtgttttc cgtttttgtc
    27181 aaaaccggcg gcgattccta ccggattggg gaagcggata ccggctacgg tgaggggaga
    27241 acttcggttc aggcttcggc gcaacaagga ttcagcgccg ggaatgtgca gcagcagttc
    27301 cagcagggcc agcgctacgg cgtgcgcttt ttcgggcgaa agtaaaaaaa gaagtggttg
    27361 aaaaacaagg cggtacatag caggtgcagg caaaaaaaaa gccggtcgtc ttgtgtatcc
    27421 ggccggccga aatcataatc ccaaaaacta tcttgatgcg tagggggcga tgaaaaatga
    27481 acactttatt tttgccccac tactaataca attcaatttg ctggcccaaa cggagggcaa
    27541 gagctgcaaa ggtatggatt aatacaaagt aaacaaaaca aatatttaaa ttttttcctg
    27601 cgctttgatg agcagttgaa aaccatttcc gtgaatgttg acaatttcga tgttttcgtc
    27661 caagcggagg tacttgcgca gtttggttac aaacacgtcc atactgcggg cggtgaagta
    27721 atcgtcctgc ccccagattt cggtgagcgc ggtggcgcgg ggcagcacat cgttggcatt
    27781 gagggcaaac atgcgcagga gttgtgcctc tttgggcgag agtttgaatt tctcttcgcc
    27841 ggcgccgccc atgttgtaag agagtatgcg aagcgggtag ttgaagtgat acttcccgat
    27901 gttgaattcc ttgatgtcgt cgcgcaggtc ggccttgggc tgcgagcgct tgaggatggc
    27961 ctgaatgcgg tagagcagtt cttccgagtt gaagggcttg gtgatgtagt cgtcggcgcc
    28021 gattttgaaa cctttgagca catcttcttt catggttttg gccgtgagga aaatgacggg
    28081 catttcggcg tctttgtcgc ggatttcttt acccagcgta aagccgtctt ttttgggcat
    28141 cattacgtcg aggatgcaca ggtcgaatct gcctttttta aaggtttcgg agccttgcag
    28201 gccgtcggtg gctaatttga cgtcgtaccc gtgcatttcc agataggatt tgagtacgtc
    28261 gccgaagttt tggtcgtctt ctaccaggag tatcctgttg tttgccattg gttccgtttt
    28321 ttcatggatg aagagatttc cccgcccggc cggtgtctgc ctgatgagga aggaaaatga
    28381 tgaaactact gcctttgccc ggctcgcttt tgacctcaat agaacccttg tgcgccgaaa
    28441 cgatggcttt cacatagctc aatccgaggc cgaagccctt gacgtcatgg acgttgccgg
    28501 tatgcacgcg ataaaatttg tcgaagatgt gcttgcgcgc ttctttgctc atccccatgc
    28561 ctttgtcttg cacggttatt tccacgccgc cttttacgtc gcgggtcaga acggtgattt
    28621 ccgggctgtc gggcgagtac ttttcggcat tgtcgaggag attgttgacg atattggaaa
    28681 ggtgtgtcaa gtcgccctga ataaccggcc gggctgcttc gagtttgcca tcgaggcggc
    28741 cttggcgttg ttctacccgg atgccgaagt nnnnnnnnnn nnnnnnatga cctcatgcaa
    28801 attgacttcg gacaggttga gagagaactc gcggcgatca atgagcgcca tttgcagtac
    28861 tttctctacc tgactattca tacgcttatt ttcctgtttt attatgttgg caaaacgccg
    28921 aactttatct gcatcgccgc tgatcatggg gctgttgatc gagtcggcgg ccaaagagat
    28981 ggtcgcgatg ggggttttaa actcgtgggt catgttgttg ataaagtcgt tcttcatttc
    29041 cgacagtttt ttctgggtaa aaatgacgaa aatggtgtag ccgaacgaaa acaaaataat
    29101 ggccgtaaaa atgagcgcgc cgataaaatt gggcagcacg ctgcgcccca caaactccga
    29161 gcgggcgggg aagtgaatca tcagcaggcc gggagccggc gtctcattgg gaaacaggtg
    29221 tacgcggtat ttggaattgt aaatattgct atagcccggc aggctttcgc gcggctcgga
    29281 gctttctacc acatagcggt cgtccatgat gacaaagccg ttgctggcgg tggcgagcag
    29341 gccgtagttg tagcctgtat tgatgccgcg gttggccaat tcctggcgga ggatatcgcg
    29401 gagtttgtct tttttgatgc gctctgcaat gggagtttgc tgaccgccct gtagttttaa
    29461 aaaagacacc atactgcggt ttatatcgtt gcgttcgcgc acgcatttgg cgcaatcgca
    29521 ttgttcttct tcagagttga ccggcaattg ccagttctga agggcattgg cctgcaccgc
    29581 acttgcgccc ggcacgcgat tgctgcgcac ctcgttgtca aagtacgccg cgccataacc
    29641 attgatataa tattcataag cctctgcctg ctcttcgtct tctaagcgga tgaccaccga
    29701 attgagcgca tcgaacacgt ctttgtcgaa tttgacctcg ttttccttga tggccgtatt
    29761 gatcaaatcc atttgcagcc acaccacacc gacgaccgcc gcgctcatca aaccaatgac
    29821 tgccagtatg tattttttat tcataagacg tgtaaatatg aaggctgccg cgggcaacac
    29881 ggcatagaaa cgaaattccg gcagcgcttg ttttacggcc cttgtattga aattccgccc
    29941 cggcttttga aacacagcac cctgaatcgg cgtttaattc tttctgtaaa atgcaaaacg
    30001 ttatgataga agaccaacca attattgccg ccaagcctnn nnnnnnnnnn nnnnnnnaat
    30061 ctgaaaggag aaaattcctg gacgatgttc aaaatgatct cagaatttgt ggacgggttc
    30121 gaaaccctca atcagatcgg cccctgcgtt tccatcttcg gatcggctcg taccaaaccc
    30181 gaccatcctt actatcgcaa agcggtggaa atcgcatcgg tactcaccaa ggccggctac
    30241 ggcatcatca ccggcggcgg cccgggcatc atggaagcag gcaacaaagg cgcggcgctc
    30301 tccggcggtc gttcggtggg cctcaacatc aacctgccgc acgagcaaag ccacaatcag
    30361 tttattgatc ccgacaaaaa cctcaacttc cgctacttct ttgtgcgcaa agtgatgttt
    30421 gtcaaatacg ctcaggcttt tgtcattctg cccggcggct tcggcacgct ggatgaactg
    30481 tttgaggtac tcacgctggt gcaaaccaag aaaatcaccc ccctgccgat cattctgttc
    30541 ggcaccgagt tttggggcgg catgcgcgag tggattcgca ccgtcatgct cgaaaaagaa
    30601 cacaacatca gtcccggcga catggacctc attcccatga ccgatgacgc cgaggaagtg
    30661 gtgcgcatca tcaatgattt ttatgaaaaa aggctcagcg aactgggtcc caactacgaa
    30721 ctctgatgat acgctaccac gatgtcaacg gaggtctgag acaaaaatac acggcttcgt
    30781 ttcggctcga cgacctggga ctgctacgcg gatacggcat tttcgattac tttctcgttc
    30841 ggcagggcgt gcccatgttt tgggatgact acgcagatcg cttctaccgc tctgcgcggc
    30901 tgctcggcct cgaagtgccc attgccccag aggaactgcg ccaacgcatc gcaaggctgc
    30961 tggaagccaa tcgcgaacgc aacacagcca tccggctgct gctcaccggc gggtatgccg
    31021 acgacagcta cacgccggca gagccgaatc tggccatcat gcagcacccc atgccttccg
    31081 tagcgcccga gcgatacgag gaaggcatca agctgatgct gtaccgccac ctgcgcgaag
    31141 tgccggaggt aaaaagcacc aactacctca cgggcatcca catccgtgaa ttgctcaagc
    31201 aggcaggcgc gccggaagtc ttgtaccacg atgggcgctt ggtgagcgaa agtgcgcgtt
    31261 ccaacttttt cattgtcact gccgaaggcg tattggtaac gcctgccgaa caggtgttgc
    31321 acggagttac ccgcaagcac gttctggaac tggcgcagca ggcaggcatt cgcaccgagg
    31381 tacgcgatct cacattggac gaattgcctc gcgctgccga agccttcctc accagtacca
    31441 tcaaaggcgt actgcctgta gtgcaaatag atgaactgcg catcggcgag gggcggcccg
    31501 gccctgtgtc ccgacgcctt cgggagcttt ggcaaaacca tgtagctgat tatattgctc
    31561 gtcatcaacc attcaaacga tgaatgccct gcgccggaag ctgctcatcg ccttgtgctg
    31621 ttgcctcctc ggcggggcgg ctttatcggc acaaacggtc agacgcgctt ccggccctgg
    31681 tttgcaactt cctccgctcc acgtctcccg catgtcctgg cgtgtacctg cgccggaact
    31741 tcctgttctg cccaacgcca aattccagcc cgccgctcag cccaaagcct ggcattatca
    31801 ggaactggca ttgttttgca aattggaagt aaaaatggag cgcgcttttc ggatgccgct
    31861 gcgcctccgc ataggcgatg tgcaatatgt ggactggctg gaaggcaaac gacgcagtgc
    31921 gtattgaggg ggaatgttgc ctttgcgcgt ggttgtttcg nnnnnnnnnn nnnnnnnnnn
    31981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32101 natggttcga ggtttcaggt ttccctcgca ttcgcggaat ttcgcgccct tcgctaagtt
    32161 tcgcgttaaa aactttggag ttccggcagg aacgggcttg gggttcgggg gttcgggttc
    32221 ggggtttcgg gttgctcacc cctcacacac tcacccctca ctcctgaaaa ccgcccacca
    32281 ttttcgagtt ccgcagcttg cggcagtctt ttcactctgt agaaaaaggt catccatgaa
    32341 atacattttt tacgtcgcca tcacgctcct tttttccggt attgccgcca aggcacaaac
    32401 gccgctcagc atcgaacagg ccattgcact gggaatacaa cgcaacctgc cgctgcaatc
    32461 ggcccgcaac aacgcagaaa ctgcccgaat caacaacgac tggggcgccg cgggccgtta
    32521 ccccgacgtg aacgccaccg tcaacctcaa caacggatac aacaacaacc aaaacccggc
    32581 ctcctttctc caatcccttt ctgccctcaa cacaggcatc acgcccggcg tggaagcagt
    32641 atgggtggtg tatggcggcc accgggtgca tatcagcaaa gaacagcttc aattgattga
    32701 aaatcaagcg cagggcaatg tgcggcaggc cgtggaagct actattgaag acgtcatttt
    32761 gagttattac caggccgtta tccaacagga acaattgcag gtacgcgccg aagtgctgcg
    32821 tttgtcgaga gagcgcctcg aatatcagcg cacccggcag gaattcggac agtcttccac
    32881 gttcgatgtc attcagacgc aagacgcctg gctcaacgac tcgactgcgt atttggtaca
    32941 acaaaccaac gtagccaatg ccatgcgcaa cctccgccgc gccatcggtg aagacaaccc
    33001 cgaaactgcc tacgcgctga ccgaccgcct gccggtagaa acaacccgat acgacgccga
    33061 agaactgcgc acccgaatgc tcaactccaa tcaggcgctg gcccagttgc gcatcggcag
    33121 agaattggcc gccgtgcaaa cccgcttcca ggagagtttc aacaagccca cgctgagcct
    33181 gcgttcggga gcctcttaca ccctgagccg caatattttt gccaacggcc tttttgccaa
    33241 cggcgaactg cgagacctcg gcggcatcca aaacaccaac ttcaacctct ttgtgaacct
    33301 cagcgccgct tacaaccttt ttgacggcgg cgcacggcgc cgaaatgtac aaaccgcccg
    33361 catagccgaa ctgaacaccc aactcggcat ggacgacatg aggcgcaatc tcctgacaca
    33421 attagacata gccctggaat cttacaacaa tcaaaaacgc gtgctggaac tgagcaacca
    33481 gctggtgaac aatgcccggc gcaatatcca gattgccgaa gaacgcttcc gcggcggcct
    33541 catcagcttc ttcgattacc gctccattca aatctcctac atcaacgcta cgcaaacccg
    33601 cctcaacgcg ctgtacaacc tcaaaaccac cgaaacggaa ttgctgcgac tgagcggcgg
    33661 attggttcgg tgaggggtat tgtcataaaa acaacttggg cggcatgtaa aaaacctacg
    33721 gttcggtatt ttgtgttaaa tttgtgtaaa tttttatttc ccaaaacaaa cacaccatac
    33781 catgaagcct ttacagtcat tgtttttttt cacttgcgca ttttgcctca tttctctcca
    33841 aacgacacat gctcagaatt tccgcgccgt ggacgacacc gtgtgcatcc aactcggcgt
    33901 gccgttcacg ttcaacgtca aagacaatga ctcgataccg gcggggggca atgccttagt
    33961 cgttcccctg tttcaatcca actgcgtacg actgctcgaa aacggagaac tccggctcat
    34021 ttccgacagc cccgattgct gcggcactta tacattcggc tatcggtacg aaaactgcga
    34081 tgatcccgct tgttttgcaa aaattgaact caccatcaag tgccccaagc cagattgttc
    34141 ggtcattgat ttgagcgact atttaaacag gagtagttcg ggcaaccccg atttacaacc
    34201 cgcctgcgtg gatgtgtgcg ccgaatcgga ggtcactttt ttcctgccga acctgccggg
    34261 attctcttac aactggagtg catcgggagg cgtggtttcg ccgggcagcc atcctgccga
    34321 ggtaaacatc gtctggggac cggccggaac gggcaacatc agcattacca ttaccgacag
    34381 tctcaatgtg ggcatcaccc gcaatatttg tgtcaacata ttgcccaaac ccacagcgtc
    34441 atttaccaaa tcggccactt cgatatgcct cggcggctcc gtcactttta ccaatacttc
    34501 caccggcggc aacagttatt actgggattt cggcgacggc agctcttcgg ctatgttctc
    34561 gcccacgcac gagttcaact cgcccggtac ctacgatgtc tgtttgtatg taaccaaaga
    34621 caatgtggac ccgcgcggca atcccctttg ctgctgcacc gatacgatgt gcatgaccgt
    34681 cacggtggac tcattgcccg gcccgaatat ctattggatt tctacccttt gcgccggaga
    34741 cagcagcaaa tactggaccg acgccgccaa ctgcgcctct taccaatgga cggtgctgga
    34801 ccacaacggg ctaccgctga gttttaccgg tcagggcaac gatacgattt gcgttgtatg
    34861 gggcaatggg ccgttcggaa cggtcacttt gcaggtgtcg ggctgtagcc aaacttactg
    34921 cgacgagccg gtgactgccg ttgttcccat catctcgccc acggcggtgg tgagcggccc
    34981 cacggtagtt tgccaaaatg aaacagccac ttacaccctg cccaaatggc ccggtgtgta
    35041 ttatgactgg caagtgggtg gaggtaccgt cgtttcgggg cagggtacca atgtcgctac
    35101 gatacaatgg gggccggggc cgacaggcac gattacggtg aattacagca gcccgttttt
    35161 acagggcttg ccgggccaca gtccgcccga ttgcagcggc acggccacgc tgaatgtgca
    35221 gatcagacct aagtttacag tacaagcatc gcccagcgtg gtgtgtgtca attccacctc
    35281 cagcgttttt gccttgcctt tcacgagtta caactggacg gtcagtccgg cggcactttt
    35341 ctccgggcaa ggtaccggcg ccatcaacat caactggaca ataccgggta cttataccgt
    35401 cacggcagnn nnnnnnnnnn nnnnnnnnnn attattgcaa cagcagcgcc tcggtcgtca
    35461 ttcgggtatt agatgtgccc gctcccgact ccatcaccgg gccgctgctg atatgccccg
    35521 gcgaaacgca aacctatttc ggccatacat cggcttcggg tacggtactg aattggacgg
    35581 tcaccggcgg cacgcccact acgggtaccg gcaactccat caccgtcacc tggggcggtt
    35641 cggggctata cactttgtca ctttcgcaaa gcctcacggg gcagccgatg tgcagttcgc
    35701 cggccataca actgaccgtg caggccaaac aaatcgccgg gccgcttacc atcagcgggc
    35761 cttctggctg catcaacagt acgcagatgt attccgccgg ccccgcgcaa caccccgatg
    35821 ccatgttcaa ctggaccatc tcgccggcta cagcgggcag cgtggtggca ggtcagggta
    35881 cgcccaatat tgacgtgcaa tggaacaaca ctcccggcac tcccatgctt acggtagcgg
    35941 tgaccctctg cggcgtcacg caaacgcaca gcattccggt aacgcttaat ccgccggtgg
    36001 tacctgtcat tacccaaacg ggcgtgctct gccccggtag cagcgtcact ttggatgcag
    36061 gcagcgggtt cagcggctat ctatggtcca atatggctac taaccaaacc attaccgttt
    36121 ctacgccggg gtcttatacc gtaaaaacca tcgacgccaa cggctgtatg attaccgtgc
    36181 cgtatcaggt ggtacctgcc ccggggcctt tggcatccat ctcatcaggc agccctacct
    36241 tgttgtgtat caatccgccc acaccggcat catccacact gatagcactg actcagcccg
    36301 gcaataccta cgaatggttc tgtaacggtg tatctcaggg cgcctcctca ccgacggcta
    36361 ccacttttac acacaacaat accaatgtgg ccgccacttt caactactgg gtggtggtga
    36421 ccgcccccaa cggctgtatc tctacgtcga acagcattac cgttgttcag gatatttgcg
    36481 acggagacgg ctgtacgccc aacggcccgc acagtctgtc tttcactccg gccaatcagt
    36541 ttcccaattg caacgtggtc aatttttcca ctacttcctc caatgtgacc ctcacaggat
    36601 ggaatttcgg cgatccgggg aattccggca ccctggcagc cgcgcaacat acctacacgc
    36661 aagccggctg tttttatgtg gaactactgg gtactacgcc gggcaccctc tcagacggaa
    36721 cgccggccaa ctgcttggtg ggcttcaccg acagcgtgtg cgtaccggta gcagcccatt
    36781 tcagcgctgt gatcggttgt tcgcctgtgc cgtttacggt cacctttacc aatctgacga
    36841 gcatcctgcc gggcgaaacc atttccggct acttctggga ctttgatggt accaatacca
    36901 acacctctac ggcggtcaat ccggtttaca gttatgcctc tgcgggcaat tataccgtaa
    36961 cgctcaccgt cacattgatg ggcggctgtc aggctacgat tactcaaaat gtaatggtga
    37021 cgggactggg cgcgccgagt atttccatca atcccaatcc gccctgcgcg gggcagcctg
    37081 tgcaatttat gggcatggct accggcagtg tcatcagttg gttgtggaat ttcggcaacg
    37141 gcgcctccaa cggcagccag agtccgatgc aaacctacct catggacggc ccttacacag
    37201 tgaccctcac cgtcatggat gcacaaggtt gtaccaactc ctccacccaa aacattgtag
    37261 tgcaacccac gccctccggc gtcatcacgc ccagcactac cgcgccggta tgcgccggcc
    37321 aaagcgtgac gctgacggcc cccgccggaa cgggctacac ctacctgtgg agcgacaact
    37381 ctaccggccc cacgctgacc gtcacgagca gcggcactta cagcgtgcag gttacgcttg
    37441 gcccctgttc ttacaccacg cccgccgtca cggtaacggt gctgcctccg ccgccgaccg
    37501 tcatcagcgg caatttggtg atctgcgata tgggctgcac cacgctcctg gcgccgccgg
    37561 gctattctta ccaatggctc gatccggtga atccgatttt ggggcaaacc ggcgcttctt
    37621 tacaggtctg ttcgggcagc ctctcgccgg cttatgcggt gtctgtgacc gacggcaatg
    37681 gttgttcggc agtatccccg cccgtaacgg tgacggtagc cgtatcgcct tcgtttgtgg
    37741 tgaacgccgt gcccactccg gcctgcgagg gtacgccctc tgtgctcact atttcgccgg
    37801 tgcaagccaa cgtggtctat tcctggagca atggcggcag cggcacgtcc atcacggtat
    37861 tgcaggccgg ttcttacacc gctaccggtg tggacacgct gaccggatgc agcagttcgg
    37921 cctttgcgat tgtgaacccg ttgccggact tgtgtttggt gccaaccgga tgttacaaag
    37981 cctgcaatcc cgacacgatt tgcggcccgg cgggtttggc atcctatcaa tggaatttca
    38041 acggcgcgcc cataaccggc gccaacagcc cctgcctcgt cgttacccaa agcggttcgt
    38101 acagcctcac cggtactaca gcggcggggt gctcggccac cagcgatact ttgattttgg
    38161 aactgatgga ctgcggctgc gagaatctgt ttgatgtgac tacaacgccc gtttccggcg
    38221 atacctgctg ctggacattg agttacaaca acagctatac cggcccgatt tcgggtttga
    38281 acatccatac acaggatgca gattttgact tcgacctgag ttcgctgcac ccgtcattga
    38341 caccggtggg cttcagtccg ggcaatcttt tcctcaccgg cgcatcctgg agcaacatgc
    38401 cgcagggcgc gctcaataac tttatcaaat tctgtctgaa aaatgtgagc aattcgccgc
    38461 agcagatcat cttcgactgg tacgacgacg tgtatgacat tgtttgttcc gatactttgg
    38521 agttggaatg cccgcttgaa cccgactgcc tctatctgct cagcgacagc atttactgcg
    38581 aaaacaaaga ggtgaaatac accttcacgg tgtgcaatcc cattgatgcg gccttcagtg
    38641 tggggtatat tttgttgcag cccgtcagcc cgaatggagt caccattacg ccgccggcca
    38701 tcaatgcttc accggccatt gcacccggcg agtgccgtac ttacacggtg atgctgggcg
    38761 gaggaacaag tatttttgga gatacgcttt gcttccgcat gttggcccac gatttcgatc
    38821 cggctacggt ggacacggcc ttgtgttgct cgctcgacac cttgtattgc atcgccatac
    38881 cggattgcga accctgcgac gacatagagg tggctgcgcg gcccataggc cccaccggca
    38941 cggggccatg ttgtttcaac gttactttga tcaacaattt ctcaggcaat tactttgacg
    39001 gcgtgggcct ctgcctcatc acgcccggcg ctactttcag cacctttgcc aacacgctcg
    39061 gttccggttg ggccaccatc ggttacagcc ccaccgtcat catgctcgat gtgataccgc
    39121 cactggggag cgcgctgcca ttgggcgcca ccaacctgcc cgtgctgtgc atcaacacgg
    39181 cgcaatttgc caatccactg ttggaaatca agtggatgca gggagattcg gtggtctgcc
    39241 gcgacacagt ggtgctggcc tgtttcccgc cctgtggtta tttctctgaa gaggaagtgg
    39301 tttgttcgcc caacggcggc tggctgtatc agggcaattt caacaacaca tcgccctaca
    39361 ccatcaacca gctcaatatg gtgttcacct cgccgcccgg cctcggtgct tacaaccaaa
    39421 gcctgcccgg cacgcctgta cctcccggcg gacaactggc attcagcatt cccattggcg
    39481 cgcctgccat ggccggcgat tcggtctgtt tcacaatggc tatgcacgac accctcaatg
    39541 tggagtgttg caatgtgcgg cattgtattg aactgccgga atgtgccagc ccctgcattg
    39601 attccacgca aattgatttg acgctaccct gcttggccat ttacgatccg gtttgcggct
    39661 gcgacaacgt gacctactcc aatgcctgcg tggcgcaaaa tcactttggc gtcacgtcct
    39721 ggacgagcgg cgcctgcccg atggatcaga tcagannnnn nnnnnnnnnn nnnnnnnnnn
    39781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    39841 tcacctacag atttacgcca cccgccgacc tgctcgtctg cgacggcgtg cgctggcgtt
    39901 tccgacgctt ccccattacg gtggcttccc cctggacgaa catgggaacg accagcgcca
    39961 atgcgccttt gacttacacc tttgccggca ccggcattta tcaggtgtgc atggaggtgc
    40021 agcgcactac gcccaacgga gaggtgtgca gccgcgaggt gtgtgtattc gtcaatgttt
    40081 tcgcacaatt gcgggacgac cgcggcgaga tgctcagcat tgcccccaat ccattcaacg
    40141 gagaactgtt tgtctcctcg ccggcgccgg aaagtatgca aatggatgtt ttggtgcggg
    40201 atgtattggg taaagtgctg ttgcgtttcc gcaaagacga actgcgcgca ggcgaggctt
    40261 ggccactcga tctgacgggg ctggatgccg gcgtgtattt gctggagtgt accggcggga
    40321 accgaaaaca ggtcttgcgc gtggtgaagc agtgatggtt gcggcggtgg agtgtacgtt
    40381 tgcgaaactt ccaaaagttt cgtaaacgta ccgcactgat tatccatatc attttttcgg
    40441 gcttagtgaa ggggtgatga tctgtgccgt cgcaaggcaa atcacccctt cactaagctg
    40501 ctggcgcgag attccgctgg cgcgagattc catctcgtgc cgctatccgg cgggttcata
    40561 cccgccaaat actcatcttc gcaggtgttt tatacgcggt tggaaaccgc ataatacgca
    40621 cgcgagatag aatctcggcg cgagcagtcc gcacctttgc gaagcctgca aaaaggattc
    40681 gtaaacgtac cacactggtt atccatatca ttttttcggg cttagtgaag gggtgatgat
    40741 ctgtgccgtc gcaaggcaaa tcaccccttc actacacatg tacggcccac tacgattacg
    40801 aatcctgaaa ggcttcgcaa tcgcccggca gccggctaaa gtgaggcgct aagcccaact
    40861 tacctccccc cctcaatgat gatgatgtac cgcctcgccc cggcgagcat gatgcagggc
    40921 gtggcgcaac cacttgtacc atgcctccat gccttcgccg gtgtacgccg acacttcgat
    40981 gaagctgagg tggtgattca cctgccgtgc atacgccttg gctttttcca catcgaaggg
    41041 aacgtagggc agcaggtcgg ttttgttgat gatgcacagg tgcgccgagt ggaacatatc
    41101 ggggtatttg atgggcttgt cgtcgccttc cgtcacgctc atgatgacca cgcgggccgt
    41161 ttcgcccaga tcgaacatgg ccgggcatac caagttgccc acgttttcta tcatcaccac
    41221 ggcgccctcg gcagggttca gctttttgag ggccttgttg atcatatcgg agtccaaatg
    41281 gcagcctttg ccggtattga tctgcaccac cggagcgccc gtagcggcga tgcggtcggc
    41341 gtcgttcatg gtttgctggt cgccctcaat gacgaaaacg ggcatctcag tgcctatgtc
    41401 gcggatggtg cgctccagca gggccgtttt gccggagcct ggcgaactca cgagattgag
    41461 tgccagtatg tttttggcat cgaaatagcc ccggttgcgc tcggccaaaa ggttgttttt
    41521 ctgaagaata tcctgctcca actcgatcac cgtcttctcc ccgtgatggt ggtggtcgtg
    41581 cccgtgttca tggccatgat ggtggtggtg ttcatggccg tgttcgtgat gttcgtgggc
    41641 atggccttcg gcatgggtgt gaatggtgac ggcttcttcc ggttgattgc atccgcaggt
    41701 ggtacacata ggttgttgta ttttatagga ctttggtaaa gctgttagct tttagccgat
    41761 agccattagc ttgattgttg tgaaaaatga ttctcctttc acaggaggca ttatgcagct
    41821 tgcagcttga ggcttgcagc cgacacatcc cg
//
LOCUS       DAORLF010000004        33513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_686_length_33513_cov_2.133242, whole genome shotgun sequence.
ACCESSION   DAORLF010000004 DAORLF010000000
VERSION     DAORLF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 33513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_686_length_33513_cov_2.133242"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..494)
                     /locus_tag="PK198_00965"
     CDS             complement(84..494)
                     /locus_tag="PK198_00965"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF37331.1"
                     /translation="MIGVIIDSNVIFSALRGGKSKAREIILNKSDHLYFAPNYLIGEI
                     FKYKAKILKNSQASEEETLEFLFKILHKIQFVNEEDISTANFIAAYRLCKNTDEKDTP
                     FVALSLELDCPLWTRDIQLKNGLRALGFDNFFEE"
     gene            complement(491..715)
                     /locus_tag="PK198_00970"
     CDS             complement(491..715)
                     /locus_tag="PK198_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37332.1"
                     /translation="MKSITFDTLDDRFVISIEKNLVAKDRLLQLLNDLRIELLADKVD
                     FGSQLEELDETIKQQWWDQNKRRFIPESVL"
     gene            complement(792..>2066)
                     /locus_tag="PK198_00975"
     CDS             complement(792..>2066)
                     /locus_tag="PK198_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF37333.1"
                     /translation="PPCLLYSDGQGNVFEDTSLYAAGRSGHYVDPVPDEDWIELPEGG
                     NLYELPNRRGLGIDVKTGEIRLCDKGWAVAAFVPPAYTGLYIAPFANEEGAQPLPLFC
                     YTTVGYHKGKNYVPAVRIEQDIRQECAGFDQNQVELGIEDLRRQFPQNRIVEHLADNC
                     ALTYHCPAARNYFMGRWECPVPTSPACNSNCIGCISMQPQEEPVPSPQFRLDFKPSAG
                     EIVEYVVPHLENAPFPIISFGQGCEGEPLLMWETLREAIVQIRNFTPKGSININTNGS
                     KPAAVEALCKAGLNSIRVSTNSMQPDMYYRPNNYRFEEILESLRVVNRYGGWTSINYF
                     VFPGVTDTEAEYEALREAIRSTGLKMIQWRNFNIDPDWYLQRVGLEEPGDPIGIKNMM
                     EALQEEFPHLKYGYFNPPVERIAGDYEKDFAH"
     assembly_gap    2067..2076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2376..>2937
                     /locus_tag="PK198_00980"
     CDS             2376..>2937
                     /locus_tag="PK198_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37334.1"
                     /translation="MKYLAITSLIVLFAGCAQPRYLQPAREQNSNTEILYHSGNPYIR
                     STMDNAEVSAQVLARSGRTLSLDLLCFNDGAEVLDIVPTDISVTGYSDLGIPYTFRVF
                     SAEQYIRRRNTRNAIIGGVVVVATVASAIALSDNALANGGNFNANWFLWSLSTVPSIV
                     LDSRAVSPAISNDGLARPHSLLPQSGY"
     assembly_gap    2938..2947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3050..4069
                     /locus_tag="PK198_00985"
     CDS             3050..4069
                     /locus_tag="PK198_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRF37335.1"
                     /translation="MMINLISDTVTKPTPEMLQAMMAAEVGDDVFGEDPTVRALEEKA
                     AAMFSKQAAVFCPSGTMTNQIAIKVHTRPLDEVICEEGSHVYQYEVGGYAFHSGAGIN
                     LIKGQYGKITAEQVAAAVKPLHDWLPESRLVVLENTCNKGGGSYYTVAEMAPIRDLCR
                     ERGLALHLDGARIFNALVETGDAPQQVGGLFDSISVCLSKGLGAPVGSLLLGDAEFVR
                     QARRVRKAMGGGMRQAGFLAAAGIYALDHHVERLRDDHRRAKALGAALEVLPMVENIR
                     PIDTNILIFDVKTPHTAASFLSMLADKGIKGSAFGPHTVRLVTHLDFTEAMLTKALEV
                     ISALR"
     gene            complement(4477..4800)
                     /locus_tag="PK198_00990"
     CDS             complement(4477..4800)
                     /locus_tag="PK198_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37336.1"
                     /translation="MDTGNTTKTHIHNRAERALHLLETLQKPDTSGILRLLLDHGPSS
                     FLELTIHTGMDSESLEYCLDRLCSSSLVRQKSSLLEADRFEPNLHKLRRVTAIVQQLA
                     AFHTD"
     gene            5009..>5762
                     /locus_tag="PK198_00995"
     CDS             5009..>5762
                     /locus_tag="PK198_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF37337.1"
                     /translation="MSKTSASDYLKHLKSDLPASVVVFFVALPLCLGIALASGAPLFS
                     GLIAGIIGGIVVGGLSGSKLGVSGPAAGLAAIVLAAIAQLGSYELFLTAVVLAGIMQL
                     ILGFAKAGIIGYYFPSSVIKGMLTGIGIIIILKQIPHFFGYDADPEGDFAFHQVDGEN
                     SFSELLNIADKIIPGSALIGAVSLAILILWDQVLSKKGKIFQVVQGPLVVVALGIVFY
                     IFTRSHSTLSISATHLVSVPVPDNFSSFLGQFT"
     assembly_gap    5763..5778
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5779..>6161
                     /locus_tag="PK198_01000"
     CDS             <5779..>6161
                     /locus_tag="PK198_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRF37338.1"
                     /translation="DFSAVMRYDVWVIALTLALVASIETLLSVEATDKLDPDKGVTPT
                     NRELLAQGTGNILSGLIGGLPITQVIVRSSANIQSGGKTKVSAILHAVFLLLSVVLIP
                     SVLNMIPLSVLAAVLLVVGYKLAKP"
     assembly_gap    6162..6186
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6187..6655
                     /locus_tag="PK198_01005"
     CDS             <6187..6655
                     /locus_tag="PK198_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF37339.1"
                     /translation="KLGWKQFIPFIVTVAGVVLIDLLSGVGLGMAIGIFIILLKSFQN
                     SHFLHKVDESNGKQKMKMTLAEEVTFINKGTILKELNSIPENTHLCLDVRATRYLDYD
                     VMEILDDFAIKAKNKNIDVELISERGVVVNPPSYVDFFHLRPKINPYRGEEQW"
     gene            6815..7450
                     /locus_tag="PK198_01010"
     CDS             6815..7450
                     /locus_tag="PK198_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRF37340.1"
                     /translation="MRAHSAETQSSVTPQKALQFLKEGNQRFVNNLKFNRNLLEQVND
                     TRSGQWPFACILSCIDSRTSAELIFDQGLGDVFSIRIAGNFVNQDILGSMEFACKVAG
                     SKLVLVLGHTKCGALKGGLDAVKVDDLGMDNLSHLVHRFDTCIHSVMKPGEERSSANH
                     DLLERLTIRNVEQAIVDIRNQSSVLRNMEKNGEIIIVGANYDVETGEVFWL"
     gene            7486..7926
                     /locus_tag="PK198_01015"
     CDS             7486..7926
                     /locus_tag="PK198_01015"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HRF37341.1"
                     /translation="MLDQETYIKDRVEGQIKWYSNKSSAAQKRYKLFKVITLLCAVLI
                     PFLTGLLEEIFWMKYVIGALGVAIAAVEGIQSLYKNRELWLEYRGTSEALKREKLLFQ
                     TLSGDYTEAADPFKLFVLKSEQIMAGEQSGWIDYIAKMENGDGK"
     gene            complement(8087..>8302)
                     /locus_tag="PK198_01020"
     CDS             complement(8087..>8302)
                     /locus_tag="PK198_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HRF37342.1"
                     /translation="YRQLREALPAGIRADIDAINQTMLAYPDIFPRFRDAAYNTYLKA
                     QGVQEGMLSYDKVVMLVRAERLAAAGR"
     assembly_gap    8303..8319
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8320..9158)
                     /locus_tag="PK198_01025"
     CDS             complement(<8320..9158)
                     /locus_tag="PK198_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HRF37343.1"
                     /translation="MKTSQKIWILLGAFTLLLRWIAGARPDWTERFYSRGLFIGIRNV
                     IDYGLAWLPFPLAYLVIPGVLIWVGVSVYRWYRRREGVWGKIKSAGAGILSFLGGVVF
                     FFFFLWGFNYSRIPLEQQLDLQPQPLSTEELREEFDLAATALIAARMRIPGASDAALD
                     YRPDARPLEQQLREEVAAWLQSHGYYAGGRVRGRQLYPKGALLRFGTAGIYFPFSGEG
                     HADAGLHALQLPSVLAHELAHGFGFGDEGVCNYIAYAACVRSDDPVIAYASILSFWRT
                     LAA"
     gene            9208..>10387
                     /locus_tag="PK198_01030"
     CDS             9208..>10387
                     /locus_tag="PK198_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF37344.1"
                     /translation="MKKAQNSEEKFMDISHILNHLGEDREQYFNAVAPPVVQSSNFAL
                     PSIAAFREAFSDELHHHVYTRGNNPTVEILRAKVAALEGCEDALVFSSGSAAVAAAVL
                     SQVAAGDHIVCVRSPYSWTHILMTRFLPRFGVSHTFVDGTDMAEIEAAIQTNTRLLYL
                     ESPNSLTYELQDLEACARLARAHGIVTCIDNSHASPIFQNPAAMDIDMVVHSGTKYLN
                     GHSDVVCGVVCSTKERIRKIFEAELMTLGAIPSPHDAALIIRGLRTLELRMQRSHDSA
                     LRIAEWLEQHPKVERVLHPWLPSFPQHELAKKQMRGAGGLFSIYLKAERIEEVEVFCN
                     RLNRFLMAVSWGGYESLMLPMAGFYKIPGKGTPSRPWNLIRFYIGLEDPDWLIEDLRQ
                     A"
     assembly_gap    10388..10418
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10474..10854)
                     /locus_tag="PK198_01035"
     CDS             complement(10474..10854)
                     /locus_tag="PK198_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37345.1"
                     /translation="MTTLKKIAMMAPALILFCLATPALAQKTGSTTSDEKPKGVFSVF
                     KKSDDNAGSSTSNSDVKARHQEAKKDVLVAKRERKAAEAKEEAARARAEAIKAEKRAS
                     AAENKSQKADVRAEKTRQKVGGKR"
     gene            11253..12155
                     /locus_tag="PK198_01040"
     CDS             11253..12155
                     /locus_tag="PK198_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF37346.1"
                     /translation="MTRLLTLCIALLAGLSAWSQPAVTQYAPTASENSLLWEISGNGL
                     TQPSYLFGTIHIIAAEDFLLTDSTLAAFNRCKEVAFEIDMDEMDNIGALLPLLMKAFM
                     RNGTTLRDLLSTEDYNLVKARFSEMGLPIFMLERVKPMFLSSMLTGQGEDSSSGMADS
                     KSYEMELLQLARDADKPVHGLETAAYQMSMFDSIPYKAQAEMLVQTLKAAPSENDEMA
                     QMVALYKAQDLVGLQEMMEGESGVGEYQHLLLDQRNANWIPVMSRMMKDKAVFFAVGA
                     GHLGGEKGVIALLRRQGYQMRAIR"
     gene            12263..12919
                     /locus_tag="PK198_01045"
     CDS             12263..12919
                     /locus_tag="PK198_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF37347.1"
                     /translation="MFRMLCLLIFLYCGYGISAQINVGIKLGLATTGFEEQQINILDQ
                     GGLQRFGLALRDGSYSIHGGLMLRARLGKVFHIQPELLLQSNKTDYNLDDLAAPGAPA
                     QLLTEKYQHLNLPLMLGAQWGAFRVQAGPQGHVFLSSRSDLSKVEGYQEDFEKMTLSW
                     VAGAGIDIWKTLTIDLRYEGSLNRLGDHIRFFGNSYSFEDRPARLLVSAGLLFGKKKR
                     "
     gene            12952..13725
                     /locus_tag="PK198_01050"
     CDS             12952..13725
                     /locus_tag="PK198_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37348.1"
                     /translation="MGNVISSFPNPKSPMQQSISLNSLLFLAFTLTGIATMSAQNGPQ
                     FGITAFPNYSGGRIAAGSTVNEAAIDSIELRETSRPSLSWGIVAQWRAEKIGFRTGFQ
                     LTDTGYRSVKEGIPADVEAPDGALTRRVDYRNLNLELPAEILFLHELNARHRVNFMMG
                     FSAAYNLRNYEDNLFFSGEKLGRAQNQISNDAFQRLQLGFQTGIGWQYDMGPRMIFFA
                     QPTFYFWYTGLLAETEEVNRSLYAIGLKTGILFKTKTNQ"
     gene            13729..14712
                     /locus_tag="PK198_01055"
     CDS             13729..14712
                     /locus_tag="PK198_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF37349.1"
                     /translation="MEYRRLGKSGLQVSALSLGSWLTFGKQISDDVAEQLMVTAYDNG
                     VNFFDNAEIYARGQAEVVMGNILKKTGWDRTSYVLSSKVFFGDGRKLPNQTGLSHKHI
                     IEGCEASLRRLQVEYLDLYFCHRPDKNTPIEETVRAMNLLIQQGKIFYWGTSEWSAQE
                     IMEAHLVAREQRLVGPVMEQPEYNMFHRHRVEAEYAQIYKTVGLGTTIWSPLASGILT
                     PKYLKDNPEDTRLAMAGMEWLKNSALKPERVEKAALLNELAKELGVSLPKLAISWCLS
                     NPHVSTVILGASKVSQLEETLQSAQVLPLLTPEVTERIEGILGNKPAQPAF"
     assembly_gap    14786..14826
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14952..16277
                     /locus_tag="PK198_01060"
     CDS             14952..16277
                     /locus_tag="PK198_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRF37350.1"
                     /translation="MAQRKIFFWSLTVALAGFLFGFDTVVISGADQSLQALWPRGEVF
                     HGFVVMASALWGTVVGALFGGIPTDRWGRKKTLFWIGVLYFVSAVGSALAHDPFVFAL
                     FRFIGGLGVGASTIAAPAYISEIAPANDRGKLVAMYQFNIVFGILVAFLSNFLLSQTG
                     DNAWRWMMGVEAVPAFIYTLLVLGVPRSPRWLLVKKHDRAAAAQTLRIINPGMDVEAE
                     MDRILADQKNVAPGETIFLPKYRPALMLAFFIAFFNQFSGINAFLYYAPRIFEIAGLE
                     KSSALLSSVGIGVTNLVFTLIGLSLIDRFGRRQLMYIGSVGYIVSLSLVATALHFGIQ
                     GMAVPIFLFLFIASHAIGQGAVIWVFISEIFPNHLRASGQAFGSSVHWVLAAIIPSLV
                     PYLFAGIGAATVFAFFAFMMVLQLYFVWRSMPETKGVSLEELEERLVRK"
     gene            complement(16504..16719)
                     /locus_tag="PK198_01065"
     CDS             complement(16504..16719)
                     /locus_tag="PK198_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF37351.1"
                     /translation="MPIIVNLDVMMARRKMSLNELSEKVDLTLSNLSILKTGKAKAVR
                     FSTLEAICKALDCQPGDILEYEEEEEG"
     gene            complement(16723..>17055)
                     /locus_tag="PK198_01070"
     CDS             complement(16723..>17055)
                     /locus_tag="PK198_01070"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRF37352.1"
                     /translation="GMVSLVIALACTKVALAWRAVDMSLIFKVENPFNERHVKLLSKM
                     SDTALSAAMLSLAGAGYSKWLMHRGVELPPSDWAAGEWLFWAGIIFLLSVVFEKGVEL
                     KSENELTI"
     assembly_gap    17056..17138
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17495..>17754
                     /locus_tag="PK198_01075"
     CDS             17495..>17754
                     /locus_tag="PK198_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37353.1"
                     /translation="MSRTDYLTLAIVAVCIAALAFLIYRMATIDADPAATDPTEQVLD
                     EPASDPTMTDENTAGTEYGDETPAPDYSRDADEMDDAKVESV"
     assembly_gap    17755..17764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17765..18106
                     /locus_tag="PK198_01080"
     CDS             <17765..18106
                     /locus_tag="PK198_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF37354.1"
                     /translation="PAAKKDPAATKPAEKPAPRAEPAPPAEEPAPAPASSASGSYMVL
                     AGSFREMANAEAMVAKVKKLGYESASVEKFDRGAFAVALVDRFDSYSEANALVKELAK
                     KGVEATVYKKK"
     gene            complement(18178..>18700)
                     /locus_tag="PK198_01085"
     CDS             complement(18178..>18700)
                     /locus_tag="PK198_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37355.1"
                     /translation="LGQNQFYYKQRSMNRIVGYAVAVCCILSGCGMNDYSLCAKDYPY
                     EKKYSPYYEDAIWRLYEFNFAEIQRDSLLQQAGSIRQLEFRPMLFEVKNDTIAFYFFV
                     ILNERDLSNSLWSGAAFDPQAPEKIEMLLGERRWFTIMRNEESVEFVNFVKNNRDSLP
                     DVLECLAIKKGVW"
     assembly_gap    18701..18717
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18907..>20366)
                     /locus_tag="PK198_01090"
     CDS             complement(18907..>20366)
                     /locus_tag="PK198_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF37356.1"
                     /translation="KLYLNANVYTGQARFADCANYCDKVITAGYTLEPEYQHLFLADN
                     HRSNEIIFPITFDGINTRTWGGMTFIINAAIGGNINPSEQGVGGGWGGTRATRQFIEK
                     FPETEGGLIASPNAGETATYPKVYVPGSHQGNNPTETSNSLSSPLRNNVFDGYKYFPD
                     ANTTIRFTRIPSNSAPIYGDTNGDGTLELNGAPITVPTAGLHYIRVNWTQLSYQIVRR
                     DWFVEGPGVSSGQPVPMTWDPTLRAVVANLNMTAAGFKFVSSDPTVNLGDNAGNGLLS
                     QGGATINRIPGSGSYRIVLFINQPDYTYQINSTSFDRRGIFHRPGQTLDIVDVTLFTQ
                     GIAVKKFKNVTSTGAQGSNATFPDTDFPMFRLADAYLMGAEALLRSGGSRSKALEYVN
                     AVRTRAFKSVAGSVTDSQLNLDLILDERARELYWECHRRTDLIRFNRFSQSDYLWQWK
                     GGVKDGAVVPVYRNVFPIPSADINANPNLQQNTGY"
     assembly_gap    20367..20523
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20829..21098)
                     /locus_tag="PK198_01095"
     CDS             complement(20829..21098)
                     /locus_tag="PK198_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37357.1"
                     /translation="MKNIKIFALLIAMFFAATGCFKDLDTVPLDPLEITSGTVYSDPA
                     VYKQVLAKLYAGFALSGQEGPAGQADISGIDEGFGQYLRGYWYHQ"
     gene            complement(21120..>22682)
                     /locus_tag="PK198_01100"
     CDS             complement(21120..>22682)
                     /locus_tag="PK198_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37358.1"
                     /translation="EGMLKIPGGNVVAFSFDPINGGGRDNTYSQTRTNELLEFYLSYK
                     ADLGAKHNLDFMAGYSWQHFYNENSFRNSNAANTPSETVERNNVANELYLVSLFGRAN
                     YSFNNRFLLTLTMRGDATSRFAPENRWGLFPAVAAAVKIIENDNQYFNNIKLRAGWGV
                     TGQQDIGSPYIYQARYQFSLDNARYQFGDFFINTVRPNGYDRNIKWEETSTTNLALDF
                     SIVKGRLSGSLDLYQRNTKDLLNFIPVPAGTNLTNFINTNIGSMVNRGVELSLNVSPV
                     STSKVRWDIAANVAYNYNEITKLTATDDPDYKGVFTGGIAGGVGSNIQIHSVGYAPRS
                     FYVFKQLYDENGSILPGQYADLNNDGVVNDEDKYRYEKPAADYLIGITSNMTIGKLDF
                     SFAGRANIGNFVYNNVATDMGYLQRLFHPTLYLQNIHRSAVDNNILTQASATFSDHFV
                     TNASFFRLDHITVGYTFDKLIGENFRAFATVQNPLVLTSYNGLDPELGTGIDNNVYPR
                     PRTFLLGVSVNF"
     assembly_gap    22683..22914
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22915..23523)
                     /locus_tag="PK198_01105"
     CDS             complement(<22915..23523)
                     /locus_tag="PK198_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF37359.1"
                     /translation="MVVDGIPLDNGGVSGNRNPLNIINPNDIESITVLKDASATAIYG
                     SRAAGGVILITTKKGAAGAKMRINYSGNVSMGNTYNRVDVLTADEFRAVMAEQYPDQV
                     GLMGDASTDWQSEIYQTAMATDHSIGATGAVGAVPYRVSLGYTMKEGLLKNDVFNRYT
                     AGVNLSPGFLNNTLQLNVGFKGMLTKNQFAERGAIGSALSFDP"
     assembly_gap    23563..23572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23573..24083)
                     /locus_tag="PK198_01110"
     CDS             complement(<23573..24083)
                     /locus_tag="PK198_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF37360.1"
                     /translation="MNANFVHRVLQIATLALCVLLPGLLLAQKTITGTVKDKSTGDPL
                     IGATVVDRDNYSRGTITDFDGSFELSVPETTAALRISYTGYASIDVPIGSQTKFDISL
                     EAGQVLEEVVVIGYGTVKREDVTGSLQTVSSANFNRGAITSPQELLAGKVPGVAITTG
                     GGPDDGARIR"
     gene            24385..>25843
                     /locus_tag="PK198_01115"
     CDS             24385..>25843
                     /locus_tag="PK198_01115"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HRF37361.1"
                     /translation="MLRLLSTLLFCAAALIGYSQITTVGIIGTATPNGWDSDTDMVQD
                     AADTAKWTLTITLVQGEAKFRANNGWDVNWGDSAFPIGTGTQGGPNIPVPAGTYNISF
                     NSVTGAYFFDLVSDIGIIGSATPFGWDREVFMFQSEADTNQYSLTLNLAQGEAKFRAN
                     GNWDVNWGAADFPAGIGTPGGPNIPIAQAGKYLIEFNKATGAYNFTEQVEFTSIGVIG
                     SATAGGWDAETALTRDSGNPDLWKGTVTLTEGELKFRANNAWAINWGGGEFPSDTATV
                     NGDNIAVPTAGDYLVSFNTRTLIYTFLLIGNYTSVGIIGDATPGGWDNDTDMTQDPDD
                     RSIWKLRIILTAGEAKFRADNDWAVNWGGGTSPNGTATQDGPNIPVTAGEYKVTFNST
                     TGAYTFEEIIEFAQLSLVGRSGPFQNWPGGGPCADINCDTYLEKNPNDGNLWSATGVT
                     LTTSADEQDGGIKFRANTDWAVNWGSAAFPAGTGTQ"
     assembly_gap    25844..25859
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25860..26183
                     /locus_tag="PK198_01120"
     CDS             <25860..26183
                     /locus_tag="PK198_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37362.1"
                     /translation="NVAFNAVTGEYAFSPASSTREDLLWPSAIQLFPNPASEFVNVNI
                     DSDKLANNIQMSVYDNFGRQVMRQTFDSKANIRLNVASLPAGMYTLSITDGKYIVGKQ
                     LVVTR"
     assembly_gap    26249..26258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26355..26930
                     /locus_tag="PK198_01125"
     CDS             26355..26930
                     /locus_tag="PK198_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRF37363.1"
                     /translation="MLLIQDILISDDVVKSHFVCKLDACKGACCWEGDYGAPLEVNEL
                     PILERIFPQVRPFLTPEGIAVIEEEGTAVYNENHETYATPLVNNGPCAYMTRDEKGIA
                     HCGIEQAWKAGAVDFQKPISCHLYPIRVSVSDIPGFEALNYDQWDICNAACSFGAQLQ
                     VPIYQFVKDALVRKYGQEFYDELHAAAQGYE"
     gene            27008..27814
                     /locus_tag="PK198_01130"
     CDS             27008..27814
                     /locus_tag="PK198_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRF37364.1"
                     /translation="MKLHFLLFLLCFAGTLSAQSTGRWITLFNGKNLDGWTAKISGYE
                     AGDNFANTYRVENGVIKTAYDGYDDNFNNRFGLLFYEKPFSHYRLKLKYRFTGEQCKG
                     VAEWAWRNSGVMIHSQSPQSMPRDQDFPISLEVQFLGGDGTNERPTGNLCTPGTHVVI
                     NGKLEEQHCITANAPTIPGDRWVRAEVLALGDSLIQHIIEGKVVISYSKPVIGGTVVS
                     KYDPAAKKDGMPLTGGYIALQSESHPIEFKQIKLMILDENGRPVRTQKRK"
     assembly_gap    27985..28150
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28151..28686
                     /locus_tag="PK198_01135"
     CDS             <28151..28686
                     /locus_tag="PK198_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF37365.1"
                     /translation="SVVLTGGHYNSWELMAMVFDVQAPHQTIAVYSPLSNSFFDNILK
                     ASRGKFGTILVPKNDIGDFIKQQQGQLNLYILVADQSPTYAKKVYWTKFLNQETAIAT
                     GPEKLAKLFDMPVVFCHVYKLRRGYYEVILEDVEPRPKEALPGSITEGHVRLLEKDIM
                     TAPQYWLWTHRRWKRQK"
     gene            complement(28864..29823)
                     /locus_tag="PK198_01140"
     CDS             complement(28864..29823)
                     /locus_tag="PK198_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37366.1"
                     /translation="MTKPLLTLLLFLCCSAAVWAQGYSSPVAYGTALFNEHALVSNKN
                     MDYLQYSVHSDDYSLIEQKRMALIQQIQQSLINVRTMPAYEGDSKLRDEIAAVFEAYL
                     ASFKVEFQQVNLLRRTSTESYIAMENYFKANAEAEKKIDAASKRASAAQVAFARKYKI
                     QIEEGEEDAGGGTLSQLNDYYRAVFLRVFKLSKKDAEFSDALKEQNAGKMDRYRKELD
                     VICDDHLYFFSKLPDFNGDTAYRDAALALTKFYKDLAVNGYKTMTDVIRKADKRTNED
                     VEAYNAVIVKLNEELAPLHNAVNESGDQLYKKNVPKPIETRRI"
     gene            29991..30557
                     /locus_tag="PK198_01145"
     CDS             29991..30557
                     /locus_tag="PK198_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37367.1"
                     /translation="MFKLYFITIIAVLLLCSPLSAQRQYWAGAGLELAETQGLTGWAF
                     GAQTGVAWRRSYTGVFVRQTTALVRAENASNLPYDISMQYAGLLGAWHRPAIGALEVG
                     VGFQIGLGRAVRRWDSSLGLPNDKDAILVAVPFVGLELPLGRRFRIAAHSGYRFFGGL
                     DGIDQWHGRDFWALANSIQLRVNFVDRK"
     gene            30653..31663
                     /locus_tag="PK198_01150"
     CDS             30653..31663
                     /locus_tag="PK198_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF37368.1"
                     /translation="MPVKTLLAVLFILSGLKLLAQDQHFSQFYNHPLHLSPALAGVFS
                     GDQRFSGAWRSQWASVPVPYLTFSGAYDQKIPLPGLKNGCLAAGMLFNYDRAGDAGLN
                     WLQVGLNTAYTQRLAEALFLTAGVEAAVGQRAFDPQQLYFDEQFNGDIFDPSLPTQET
                     FLQTAAGYTDLSAGLNLLVQAPQQRSFALIGAAYRHLNRPTVRFYQQSALSLRALTNI
                     YLSGTVQTSEKIDATFALAARLQGPYREIVSGAGIRYYLKNNDLHSIAVGAGASYRWG
                     DALVSYVELWKDNWRLGISYDINTSPFRVATQRNGGPEVTLQYFIHKVKAPETFKICP
                     IF"
     gene            31648..>33513
                     /locus_tag="PK198_01155"
     CDS             31648..>33513
                     /locus_tag="PK198_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37369.1"
                     /translation="MSHFLIRCALCCALLTAALAETQAQTFKAWEKAGDKALKNKDVN
                     AAMHHYARALGLNPAHVGLGYKYAGLAQRAHAYEAARDYYLKVTASPEAGVYPQALFQ
                     LGMMEKTLGRYDQAASHFRDFIDRYGASDPARKAQAESQLEQCRRAAEMSAQPPTAEL
                     VRLGKSVNSAYTEFGPYPAGDTLYFSSLRFDNPKDKNLPPRMVSKVMSSLRSGAGRAL
                     KDGFNEDTRHTAHFARSHDGRRVYFTRCDYVNASEIRCALYYREPDRRGRLRTEIRLP
                     DPLHLPGFTATQPAVGYDSLAQKEWLFFVSDRPGGAGGLDIWASEVLAKGAFGTPEPL
                     RNINTPADDITPFFHAASKTLYFSTEGRFSLGGFDIYSSPKTAGGWAEPVHGGLPLNS
                     SFNDIYPVLEQDGVRGYLASNRPGSLYLDASNKTCCNDIYQFRLLPPPADSTIPMEQA
                     LIPPPVETPLIAEEEKLPQTLEDFLPLALYFDNDEPDKRTRRTTTQQTYGGSFEKYYV
                     RKQEYMRQYAGELKGDEQQDAEDRMEDFFENDIRRGHDFLIRFSEILLTRLQKGDQVE
                     IFIKGFTSPRAETDYNLNLGKRRISSVRNHFEEWSDGQLKPYLQSGQLVITETSFG"
BASE COUNT         7564 a         8570 c         8648 g         7880 t
ORIGIN      
        1 gtcgggtgcc tcagtcgctc ctcctgcgtc gtcactacct tcggccccct cacgcgcaaa
       61 gcgatagctg ttggatagct gctttactcc tcaaaaaaat tgtcaaaacc caacgccctc
      121 aacccattct tcagttggat atctcttgtc cataaagggc aatccaattc caacgagagc
      181 gctacaaagg gcgtatcttt ttcatccgta tttttacaca ggcgatatgc ggcaatgaaa
      241 ttggcggtgg agatgtcttc ctcgttgaca aactgaatct tatggagtat tttgaataaa
      301 aattccagtg tttcttcttc ggatgcttga ctgtttttca ggattttagc tttgtattta
      361 aaaatttctc cgatcagata attgggcgca aagtataggt gatcgctttt attcaaaata
      421 atttccctcg cttttgactt cccgcctctt agcgcggaaa atataacatt tgaatctatt
      481 atgaccccga tcataacaca ctttccggta taaaacgccg cttgttttga tcccaccact
      541 gctgcttgat ggtttcatcc aactcttcca attgtgagcc aaaatctact ttgtcggcaa
      601 gtaactctat ccgcaggtca ttgagtagct gcaagagacg gtccttggct accaagtttt
      661 tttcaatact tatcacgaac cggtcgtcca acgtatcaaa agtgatagat ttcataatca
      721 gtagtttatt atgccttaaa cgtgtgccgg ttggttttgg ttgttatgtg ccggcagaac
      781 aaacagcccc gctaatgcgc aaaatccttc tcataatccc ccgcaattcg ctccaccggc
      841 ggattgaagt agccgtattt caggtgcgga aactcctcct gcaatgcctc catcatgttt
      901 ttgatgccga tgggatcgcc gggttcttcc aagcctacgc gttgcagata ccaatcaggg
      961 tcaatattga aattgcgcca ctgaatcatt ttcaggccgg tgctgcgaat ggcctcgcgc
     1021 agggcctcat attcagcctc ggtatccgtc acgccgggaa acacgaaata attgatgctc
     1081 gtccagccgc catagcgatt caccacgcgc agactttcca gaatttcctc aaagcggtag
     1141 ttgttgggcc ggtagtacat gtcgggttgc atagagttgg tactcacccg aatgctgttg
     1201 aggcccgctt tgcacagcgc ttccaccgcg gcgggtttgc tgccgttggt attgatgttg
     1261 atgctgcctt tgggcgtaaa attcctgatc tgcacaatgg cctcgcgcag ggtttcccac
     1321 atcagcagcg gctcgccctc gcagccctgc ccaaaactga tgatggggaa gggcgcgttt
     1381 tccaagtgcg gcaccacata ctccacaatc tcgccggcgc tgggcttgaa gtcgaggcga
     1441 aactgcggcg aaggtaccgg ctcctcctgc ggctgcatgg aaatacagcc gatgcagttg
     1501 gaattgcagg ccggcgaagt aggtaccgga cattcccagc ggcccatgaa ataattgcgg
     1561 gcggcagggc aatggtaggt cagcgcgcag ttgtcggcca agtgctccac aatgcggttt
     1621 tgaggaaact gccggcgcag gtcttcaatg cccagttcta cctggttttg gtcaaaacct
     1681 gcacattcct gccggatgtc ctgttcaatt ctcaccgcag gcacatagtt tttgcctttg
     1741 tggtagccca cggtggtgta gcaaaacagc ggcagcggct gcgcgccttc ttcattggca
     1801 aaaggggcaa tgtacaggcc ggtataggct ggcggcacaa aagcggctac cgcccagcct
     1861 ttgtcgcaca aacggatttc gccggttttg acgtctatgc ccaagccgcg ccggttgggc
     1921 agttcgtaca aatttccgcc ttcgggcagt tctatccagt cttcgtcggg cacggggtcc
     1981 acatagtggc cgctgcgacc ggcggcgtac aggctggtat cttcaaaaac atttccctgg
     2041 ccgtcggagt agaggaggca tggcggnnnn nnnnnntgta gcgacatgaa tggtatctct
     2101 ttttgttgcg aacagcaaag gtactgtttg ttgttaaatc gccgttaaag tgcccgcgtg
     2161 tcacggaggt tttttgtgat aggatgtctt tttacaagcg caagagccga cagttctgcg
     2221 gcgttgcaca caaagcggga tttcgcggga gcggctcgtt cattttgaaa tgtttccaaa
     2281 cgggaaatct aattccttga cagtaaacaa gctaacagcc agtagcttac agcttgtagc
     2341 ttttggtata aattcaattc acacaaaata acgccatgaa ataccttgcc attaccagcc
     2401 ttatcgtcct ctttgcagga tgtgcccagc cgcgctatct tcagccggcc agagaacaaa
     2461 attccaatac ggaaatactg taccacagtg gaaatcccta tattcgctcc acgatggaca
     2521 atgcagaggt ctctgcgcag gtactggcgc ggagcgggcg cacgctcagc ctcgatttgt
     2581 tgtgtttcaa cgacggcgcc gaggtgctgg atattgttcc caccgacatc agcgtgacgg
     2641 gctacagcga tctgggcatc ccttacacgt ttcgggtgtt ttcggcagag caatacatcc
     2701 ggcggcgcaa tacccgcaat gccatcatcg gcggcgtggt agtggtggct accgtagcga
     2761 gcgctattgc tttgtcggac aatgctttag ccaatggagg caatttcaac gccaactggt
     2821 ttttgtggag cctgagtacg gtgccgtcta ttgttttgga cagtcgcgct gtctcgccgg
     2881 ccatttccaa cgatgggctg gcgaggccgc actcgctgct gcctcaaagc ggctaccnnn
     2941 nnnnnnntgt ggtcattcag ggcaagcagg aatacatgca ccgtatagat atgaccgtgc
     3001 cggtgaacgg ggtgccgcac cgcttcagct ttttaggaaa taaccaacga tgatgataaa
     3061 tttaatcagc gacaccgtta caaaacctac gccggagatg ctgcaagcca tgatggcggc
     3121 ggaggtgggc gacgatgtgt ttggagaaga ccccacggtg cgggcattgg aggaaaaagc
     3181 ggcggctatg tttagcaaac aggcggctgt gttttgccct tccggcacga tgaccaacca
     3241 gatagccata aaagtgcata cccggccatt ggatgaggtg atttgtgagg aggggtcgca
     3301 tgtgtatcaa tacgaggtgg gcggctatgc ttttcattcc ggggcgggca tcaatctcat
     3361 caaagggcaa tacggcaaga tcacggcgga acaggtggct gctgcggtga agcccctgca
     3421 cgactggctg ccggagagcc gcttggtggt gctggaaaat acctgcaaca aaggcggcgg
     3481 cagttattac acggtggcgg aaatggcccc gatacgcgac ttgtgccggg aacgaggtct
     3541 ggccctgcat ttggacggtg cgcgcatctt caacgcattg gtagaaacgg gcgatgcgcc
     3601 gcagcaggtg ggcgggttgt tcgacagtat ttccgtgtgt ctttccaaag gattgggcgc
     3661 gccggtaggc tcgttgttgc tgggcgatgc ggagttcgtt cggcaggcgc ggcgggtgcg
     3721 caaggccatg gggggcggta tgcggcaggc gggttttctg gcggcggcgg gcatttatgc
     3781 gctcgaccat catgtggagc ggttgcgcga tgatcaccgg cgggcgaagg cgctgggcgc
     3841 tgcattggag gtactgccga tggtggaaaa cattcggccc atagatacca atatcctgat
     3901 ttttgatgta aaaacacccc atacagcggc gagctttctg agtatgctgg ccgacaaagg
     3961 catcaaaggt tcggcattcg ggccgcatac cgttcgcttg gtgacgcatc tggactttac
     4021 cgaagccatg ctgacaaagg cgctggaagt gatttcagcg ctgagatagg cggtgtgtag
     4081 tgaagggatg acgttactat tcagcgccct gcaaaaagct gcgagccgcg agtttgattt
     4141 tccagaatga aaaaaaacgc tcgttttcag agggagattt tgaaatttcc ccaaatggaa
     4201 aatctaattt ttcaacgagt aaacaaactc ataccacgcc gagggcgtgg tcataactcg
     4261 tagcttttgc ggatcggcta tagatacggg gtgacttagt ccggttcaaa actccgccat
     4321 ccccccttca ctacacatag tagtggggca aaaataaatg ctcattttct ttaggccaaa
     4381 ggattagata agacacttaa aatgaaacgt ttacttatct aatcctttgg cgaaaatgat
     4441 cactttattt ttttatcgcc ccatagaaat tatacctcag tcggtgtgaa acgcggccaa
     4501 ttgttgaacg atggccgtta cccgcctcag tttgtggagg ttgggttcaa accggtcggc
     4561 ttcgagcaaa ctgctttttt gtcgtactaa cgacgaagag cagagccgat ccaagcagta
     4621 ttccaaggat tcagaatcca tgccggtgtg aatggtcaac tccagaaaag aggatggccc
     4681 gtgatcgagc aggaggcgca gaatgcccga agtatcaggc ttttggagcg tttccagcaa
     4741 atgcagggca cgctcggcgc ggttgtggat gtgtgttttt gtagtgtttc cagtgtccat
     4801 ggtgaaataa atgatctcac gtgagggagc aaagatagca atgttattga aagttgtatg
     4861 caggcagaaa taattagttt tcagagagca gtattcagaa aaaaataatg aaatagctga
     4921 accctttttt cgacttgtgg tttttatgct tgcgaatagt tgtattcgtg ttaatgatag
     4981 tattgcaatc tttatttaac tcgtcaccat gtcaaaaaca agcgcctcgg actacctgaa
     5041 gcatctcaag tccgatttac cagcatctgt agttgtattt tttgtagccc tgcctttgtg
     5101 cctcggtata gctttggcca gtggcgcgcc tttgttttca ggtcttattg caggtattat
     5161 aggaggcatc gttgtaggcg gattgagcgg ctccaaattg ggggtgagcg gtccggcggc
     5221 aggtttggcg gccattgtgt tggcggcgat tgcccagttg gggagttacg agttgttttt
     5281 gacagccgtg gttttggcag gcatcatgca gttgattctc ggttttgcca aggccggcat
     5341 cattggctat tattttccgt cttctgttat taaggggatg ctgacgggca tcggcatcat
     5401 catcattttg aaacaaatac ctcatttttt cgggtatgat gccgaccctg agggagactt
     5461 cgcgtttcat caggtggatg gtgaaaattc tttttccgag ttgttgaata ttgccgacaa
     5521 aattattccc ggctccgcac tcatcggggc ggtcagttta gccattctca tactatggga
     5581 tcaggtgttg tctaaaaaag gcaaaatatt tcaagtggtg cagggcccct tggtagtggt
     5641 cgcattaggt attgtgtttt atatatttac gaggtctcac agtactttgt ccatctcggc
     5701 tactcacttg gtaagtgtac ctgtgccgga taatttctcg tcgtttttgg ggcagtttac
     5761 ttnnnnnnnn nnnnnnnncg acttcagcgc agtgatgcgg tacgatgtgt gggtcatcgc
     5821 attaacgctg gctttggtgg ccagtattga aacgctgttg agcgtagagg cgaccgacaa
     5881 attggacccc gacaaagggg ttacgcccac caatcgggag cttctggcac agggtacggg
     5941 caatatcctt tccgggctga tcggcggttt gcccatcacg caggtgattg tgcgcagttc
     6001 ggccaacata cagtcgggag gcaaaacaaa ggtatcggcc attttacatg ctgtgttcct
     6061 gttattatct gtggtgctta ttccctccgt actcaatatg attccattgt ctgtattggc
     6121 agcggtgttg ttggtggtag gttacaaatt ggccaaaccg gnnnnnnnnn nnnnnnnnnn
     6181 nnnnnngaaa ttaggttgga agcagtttat cccctttatc gtcacggtgg caggcgttgt
     6241 gttgattgat ctgctgtcgg gcgtaggatt gggcatggcc atcggcattt ttatcatcct
     6301 gttgaagagc tttcagaatt cacacttcct gcacaaagtg gacgaaagca acggcaagca
     6361 gaaaatgaaa atgaccctgg cggaagaagt aaccttcatc aacaaaggca ccattctcaa
     6421 agagttgaac agcattcccg aaaacaccca cctgtgtctg gacgtgcgcg ccacgcgcta
     6481 tttggactac gatgtgatgg aaatcttaga tgattttgcc atcaaggcca aaaacaaaaa
     6541 tattgatgtg gaactgatct ccgagcgcgg tgtggtagtg aatccgccca gctatgtgga
     6601 ttttttccac ctgcggccca aaatcaatcc gtacaggggc gaggagcaat ggtaagctgc
     6661 cttcggcagt ttatctccct tcggggtaag ctgcctgcgc agttcatcgg caacccggaa
     6721 cccgaaaccc ggaacccgcc ttcgccaagg cttcggcgga tgcaacccga aaccttgaac
     6781 ccgaaacttt taaactgtaa acaacaacca taacatgaga gcacattcag ccgaaacaca
     6841 gtcttccgta acgccgcaaa aggcattgca atttctcaaa gaaggcaacc agcgctttgt
     6901 gaacaaccta aaattcaatc gcaatctctt agaacaggtg aatgatacac gctcaggtca
     6961 gtggcctttc gcctgcatcc tgagttgtat tgacagccgc acctctgccg agttgatctt
     7021 tgaccagggg ctgggggatg tgttcagcat tcgcattgcc ggcaattttg tcaatcagga
     7081 catattgggt tctatggaat ttgcctgcaa agtggccggt tccaagttgg tattggtttt
     7141 ggggcatacc aaatgcggcg ccctcaaagg tggcctcgat gccgtgaaag tggacgatct
     7201 gggtatggac aacctctctc atttggtgca tcgtttcgat acctgcatcc attcggtgat
     7261 gaagccgggc gaagagcgca gttcagccaa ccacgacctg ctggagcgac tgaccatccg
     7321 gaatgtggaa caggccattg tggatatccg caatcaaagc tctgtgctca gaaacatgga
     7381 aaagaacggc gaaatcatca tcgtgggcgc caattatgat gtagaaacag gagaagtttt
     7441 ctggctttga cgagaatcct tacctttggg taaaatctca tcatcatgct cgatcaggaa
     7501 acctacatca aagatcgcgt tgaaggccag atcaagtggt actccaacaa aagcagcgcc
     7561 gctcaaaaac gctataaact gttcaaagtc attacgttac tttgcgcggt actcatcccg
     7621 tttctcacgg gactgttgga ggaaatcttc tggatgaaat acgtcatagg ggccttgggc
     7681 gtcgccattg ccgctgttga gggcatacaa tctctgtaca aaaacaggga attgtggctc
     7741 gaatatcgcg gcacttctga agcgctcaag cgcgaaaaac tcctttttca aaccctttcc
     7801 ggagattata ccgaagcggc cgatccgttt aaactcttcg ttctgaagtc ggaacaaatc
     7861 atggcaggcg aacagtccgg ctggatagac tatatcgcca aaatggaaaa cggagatggg
     7921 aagtgaggat atttatagct tcgagccgtg agcttcgagt tgcgttttac cttacttcta
     7981 ttgtggaatc tctacattta aatatcaggt ataaaaccct attttactga aaatcaggct
     8041 aacagccagc agctaacagc tatcggcctg taccccgcga ctgctactaa cgtcccgcag
     8101 cagccagcct ttcggcccgt accagcatca ccactttatc ataactgagc atgccctcct
     8161 gcacgccctg cgccttgaga taggtgttgt aggcggcatc ccggaaacgg ggaaaaatgt
     8221 cgggataagc cagcatggtt tggttgatgg catctatgtc ggcgcggata ccggcgggca
     8281 gggcttctct caattggcga tannnnnnnn nnnnnnnnnt gggccgcgag ggtgcgccag
     8341 aaactcagga tggaagcata agcaatgacc ggatcatcgg aacgaacgca ggccgcataa
     8401 gcgatgtaat tgcacacgcc ctcatctccg aaaccgaatc cgtgcgccag ctcatgcgcc
     8461 agcaccgacg gtaattgcag cgcatgcaga cccgcatcgg catggccttc gccggaaaaa
     8521 ggaaaataaa tgcccgcagt gccgaagcgg agcagcgcgc ctttgggata gagttggcgg
     8581 ccccgcacgc gaccaccggc gtagtagccg tgcgattgca accaggctgc tacttcctcg
     8641 cgcagttgct gttctagcgg gcgggcgtcg ggccggtaat ccaatgccgc atcggaagcg
     8701 ccgggaatgc gcattctggc ggcgatgagt gccgtggcgg caaggtcgaa ttcttcgcgg
     8761 agttcttcgg tgctgagcgg ttggggttgt aaatcaagct gttgctccaa tggtatgcgg
     8821 ctgtaattga acccccacaa aaagaaaaag aaaaacacca cgccccccaa aaaggataaa
     8881 atacctgctc cggcagattt gattttgccc cagactccct cgcggcggcg ataccagcga
     8941 taaacggaaa cgcctaccca tatcagcacg ccgggaatga cgagatacgc cagcggaaac
     9001 ggcagccacg ccaacccgta atcaatgaca ttccgaatac cgataaacag gccgcgactg
     9061 tagaagcgct cggtccagtc gggacgggcg ccggcgatcc agcgcagcag cagggtgaag
     9121 gcgccgagga gtatccagat tttttgagat gttttcatgc ggttggagga agtgaagcgc
     9181 taaattaccg tctcaaacaa gattggaatg aaaaaggcac aaaattcaga ggaaaaattc
     9241 atggacatca gccacatcct caaccacttg ggggaagacc gcgagcaata tttcaacgcc
     9301 gtagcgccgc cggtggtgca gagcagcaat ttcgcattgc ccagcatcgc cgcttttcgc
     9361 gaggctttca gcgatgaact gcatcaccac gtttatacgc ggggcaataa ccctaccgta
     9421 gaaatcctcc gggccaaagt agctgcattg gaaggttgcg aagacgcgct ggtgttctcc
     9481 agtggcagcg cagccgtagc cgccgctgta ttatcgcagg tagctgccgg cgatcacatc
     9541 gtttgcgtgc gctcgccgta ctcttggacg cacattctga tgacgcgctt tttgccccgc
     9601 ttcggcgtaa gccatacttt cgtggacggc acagacatgg ccgaaataga agctgccatc
     9661 caaaccaaca cccgcctgct gtatttggaa agccccaact cgctcactta cgaactgcaa
     9721 gacctcgaag cctgcgcccg gctggcccgc gctcacggca tcgtcacctg catagacaac
     9781 agccacgcat cgcccatttt ccaaaatccg gcagcgatgg atatagacat ggtggtacac
     9841 tccggcacca aatacctcaa cggccatagc gacgtggtgt gcggcgtggt gtgcagcacg
     9901 aaagagcgca tcagaaagat ttttgaagcg gaactgatga ccctcggcgc cattccgtct
     9961 ccgcatgatg ctgcactgat tatcagagga ttgagaacgc tggaactgag aatgcagcgc
    10021 agtcacgaca gtgctttgcg catagccgaa tggttggaac aacatcctaa agtagagcgc
    10081 gtgctgcacc cctggctgcc gtcgtttccg cagcacgaat tggccaaaaa gcaaatgcgc
    10141 ggcgccgggg ggctgttttc catctatttg aaagcagagc ggatagaaga agtggaagtt
    10201 ttttgcaacc ggctcaaccg tttcctcatg gcggtgtctt ggggcggcta cgaatcgctg
    10261 atgctgccga tggccggttt ttacaaaatt cccggcaagg gcacgccctc gcgtccctgg
    10321 aatctgattc gcttttacat cggattagaa gacccggatt ggctgatcga ggatttgagg
    10381 caggcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cacacactca cccctcacac
    10441 actcacccct gaaaaccgcc caccgaggac cgctcaccgc ttaccgccca ccttctgcct
    10501 cgtcttctct gcccgtacgt cggctttttg agatttattt tcagcagcag aagcgcgctt
    10561 ttcagccttg atggcttctg cgcgggcgcg ggctgcttcc tctttggcct cggctgcttt
    10621 gcgttcgcgt ttggctacga gcacatcttt ttttgcttcc tgatggcggg cttttacgtc
    10681 gctgttggaa gtggaactgc ctgcattatc gtccgatttt ttgaacaccg aaaaaacgcc
    10741 tttaggcttt tcgtcgctgg tagtagagcc ggttttttga gccagagcag gcgttgccag
    10801 gcaaaataag atcagtgccg gcgccatcat cgcaattttt ttcaaagtcg tcatctgttt
    10861 ccttttttgt gaacgcccgc aataacgaag gcgcggcgcg gtttggtgtg ccgggcagtt
    10921 tttgcattac aacttttgta caaagcactc gtttattggc ttggtaaaaa catttggctg
    10981 cgagctatga gcttcgggag gcagcgttgg acaatatcta atcatcttgt gactattttg
    11041 catctttttt aagccataac cgcgccttgg cgcggcacta gctgcaagct cattttttat
    11101 aaagtaagaa gatattccca tggggaaatt tctaaatgta cttttgaaaa aaaattcttc
    11161 tgcggtggga aaacagagct aatagcttga gccacgcaag gcgtggtaac agccaatgga
    11221 aaatgctaaa taaatacaaa atcttacaaa taatgacccg acttcttaca ctttgcattg
    11281 cgctgttggc cggcctgtct gcctggtcac aacctgccgt cactcagtac gcgcccaccg
    11341 cttccgaaaa ctcactgctc tgggaaatca gcggaaacgg tcttacacag ccgtcctatc
    11401 ttttcggcac catccacatc attgctgcgg aagacttttt gctcaccgat tctaccctcg
    11461 cagctttcaa ccgctgcaaa gaggtagcct ttgaaattga tatggatgag atggacaaca
    11521 tcggcgcgct gctgcccctg ctcatgaaag cctttatgcg caacggcacc accctgcgcg
    11581 atctgctcag cacggaggat tacaacttgg tcaaagctcg cttttccgaa atggggctgc
    11641 ctatctttat gctcgaacgg gtaaaaccca tgttcctctc ttccatgttg accggacagg
    11701 gcgaagactc ttcttcgggc atggctgact ccaagtccta cgaaatggag ttgcttcaat
    11761 tggcccgcga tgccgataag ccggttcatg gcctcgaaac ggctgcttat cagatgagta
    11821 tgttcgacag cataccctac aaagcgcagg ccgaaatgct ggtgcaaacc ctgaaagccg
    11881 ccccttccga aaacgatgaa atggcgcaga tggtggcttt gtacaaagcg caagacctcg
    11941 tggggctgca ggaaatgatg gaaggcgaat cgggcgtcgg cgagtatcaa catctgctgc
    12001 tggaccaacg caatgccaac tggattcccg tgatgagccg aatgatgaaa gataaagctg
    12061 tgttttttgc cgtcggcgcc ggccatttgg gaggtgaaaa aggcgtcatt gccctgttgc
    12121 gccggcaagg gtaccaaatg cgggcaattc gttgaggcgt tcaggggcgg attgtttaat
    12181 tttgcggcgg cctcacgatc agattggcaa tgaaaatcca atttcacagc gagccgatac
    12241 agaatgaaaa actaaaatgg tcatgttccg aatgttatgc ctgctgattt tcctgtattg
    12301 tggatacgga atttcggccc aaatcaatgt gggaatcaaa ctggggctgg caactaccgg
    12361 cttcgaagaa caacaaatca acatccttga tcagggcggt ctgcaacgct ttggattggc
    12421 cctgcgcgac ggctcgtaca gcattcacgg cggcctgatg ctgcgggcgc gcctcggcaa
    12481 agtctttcat attcagcccg aactgctcct gcaatccaac aaaaccgatt acaacctcga
    12541 tgatctggcg gctccgggcg caccggctca attgctcacc gaaaagtacc agcacctcaa
    12601 tctgccgctg atgctgggcg cacaatgggg cgcgtttcgg gtgcaggccg ggccgcaggg
    12661 gcatgttttt ttgagcagcc gctcggattt gtccaaagta gaaggctatc aggaggattt
    12721 tgaaaaaatg acactgagtt gggtggccgg agccggcatt gatatttgga aaacactgac
    12781 cattgacctc cgctacgaag gcagcctcaa ccgtttggga gatcatatcc ggttcttcgg
    12841 caattcatac tcttttgaag atcgcccggc aaggctgttg gtctcggcgg ggctgttgtt
    12901 cggcaaaaag aaaagataaa acggcgcact aaactactcg tttgtatcgt catgggaaac
    12961 gtcatttctt catttcccaa cccaaaatcg cccatgcaac agtccatttc attgaacagc
    13021 ctgctttttc ttgcatttac actcacaggc atcgccacga tgagcgccca aaacggccct
    13081 caattcggca ttactgcatt tcccaactac tccggcgggc gcattgctgc cggcagtacc
    13141 gtcaacgaag ccgccattga ctccatcgaa ctgcgcgaaa cctctcgacc ctctctgtct
    13201 tggggcatag tcgctcaatg gcgtgccgaa aaaatcggat tccgcaccgg ttttcaactc
    13261 accgatacgg gctatcgctc tgtaaaagaa ggcattcccg ctgatgtaga agctcccgac
    13321 ggcgccctca cccgccgcgt ggactaccgc aatctgaacc tcgaactgcc cgccgaaatc
    13381 ctgtttttgc acgaactcaa tgcacgtcat cgggtcaatt ttatgatggg attcagcgcc
    13441 gcctacaatc tccgcaacta cgaagacaac ctctttttca gcggcgaaaa gctcggcaga
    13501 gcgcaaaacc aaatcagcaa tgatgcgttt cagcgcctcc aactcggctt ccaaaccggc
    13561 atcggctggc aatacgacat ggggccgcgc atgatctttt ttgcgcaacc cacgttctat
    13621 ttctggtaca ccggcctgct ggcagaaacg gaagaggtga accgcagcct ctatgccatc
    13681 ggcctgaaaa ccggcatttt attcaaaaca aaaaccaacc aataaaacat ggaatacaga
    13741 agacttggaa aaagcggcct gcaggtgagc gcgctctcgc tggggtcctg gctcacgttc
    13801 ggcaaacaaa tcagcgacga tgtggccgaa caactcatgg tcaccgctta cgacaacggt
    13861 gtcaattttt ttgacaatgc tgagatttat gcccgcggtc aggccgaagt ggtcatgggc
    13921 aatattttga agaaaacagg ctgggacaga acgagttatg tgctttccag caaagtcttt
    13981 ttcggcgacg gccgcaaact ccccaaccaa accggcctca gccacaagca catcatcgaa
    14041 ggctgcgagg cttctctgcg ccgcttgcag gtggaatatt tagacctgta cttctgccac
    14101 cgccccgata aaaatacgcc catcgaggag acggtgcgcg ccatgaatct gctcatccaa
    14161 cagggcaaaa tattctactg gggcacctcc gaatggtcgg ctcaggaaat catggaggca
    14221 catctggtag cccgcgaaca gcgccttgtc ggcccggtca tggaacaacc ggagtacaac
    14281 atgtttcacc gccaccgggt agaagccgag tacgcgcaga tttacaaaac agtggggctg
    14341 ggcactacga tctggtcgcc gctggcatcg ggcatactca cacctaagta cctgaaagac
    14401 aacccggaag atactcgcct tgccatggca ggtatggagt ggctcaagaa ctcggcactc
    14461 aagccggagc gcgtcgaaaa ggcggcgctg ctcaacgaat tggccaaaga actgggcgtt
    14521 tcattgccca aattggccat ttcctggtgc ctgtcgaatc cccatgtcag cacggtgatt
    14581 ctgggcgctt ccaaagtatc gcaattggaa gaaacgctcc aatcggcaca ggtgctgccc
    14641 ttgctgacgc cggaagtgac ggagcgtatt gaggggattt tgggaaataa gcccgcgcag
    14701 cctgcttttt gataagtgat gatgtaatct tgtcaagatt tttcatcatc acggaggcac
    14761 ggaggacacg gggattacac agagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnagcg aaaatgacca ctttattttt tatcgcccct tagtgcagac aggcttatgc
    14881 ctcgtagctt tgtaagatgg actgaatttg ttgcccgaaa aactatattt gcgccaaaat
    14941 catcacaaaa aatggcgcag cgcaaaatat ttttctggtc gctcacggtg gccttagccg
    15001 gtttcctctt tggtttcgat acggtggtca tctccggcgc cgaccagtct ttacaggcac
    15061 tctggccacg cggcgaggtg tttcacggct tcgtcgtcat ggcctccgcg ctgtggggga
    15121 ccgtggtggg cgcactgttc ggcggcattc ccaccgaccg ctggggtcga aagaaaaccc
    15181 tgttttggat cggtgtgctg tattttgtgt cggcagtcgg ctctgcgctg gcccacgacc
    15241 ccttcgtttt tgccctcttc cgattcatcg gtggtttggg cgtgggcgct tccacgatag
    15301 cggcgccggc ctacatttcc gagattgctc cggccaacga ccgcggcaaa ttggtggcca
    15361 tgtatcagtt caacatcgtg ttcggcattc tcgtggcctt cctctccaat ttcctgctca
    15421 gccaaaccgg cgacaacgcc tggcgctgga tgatgggcgt agaggccgtt ccggcgttca
    15481 tttacacgct ccttgtgctc ggcgtgccac gcagcccgcg ctggcttttg gtcaaaaagc
    15541 acgaccgcgc cgccgccgcc caaacccttc gcatcatcaa tccgggcatg gacgtggaag
    15601 cagaaatgga ccgcatcctg gccgaccaga aaaacgtcgc tcccggcgaa accatcttcc
    15661 tgcccaagta ccgcccggcg ctcatgctcg cctttttcat cgcctttttc aatcagtttt
    15721 ccggcatcaa cgcctttctt tattatgctc cgcgcatctt cgagatcgcc ggcctcgaaa
    15781 aaagctccgc cctgctcagc agcgtcggca tcggcgtcac caatctcgtg tttaccctca
    15841 tcggtctgtc gctcattgat cgtttcggtc gccgccaact catgtacatc ggctccgtgg
    15901 gctacattgt gtcgctttca ttagtcgcca cagcgctcca tttcggcata cagggcatgg
    15961 ccgttcccat cttcctgttc ctgttcatcg cctcccacgc catcgggcaa ggcgccgtca
    16021 tctgggtatt catttccgaa atctttccca accacctgcg cgcttccggc caggcattcg
    16081 gcagctccgt tcattgggta ttggcagcca ttattccatc gttggtgccc tacctgtttg
    16141 ccggcatcgg cgcggccacc gtatttgcat ttttcgcctt catgatggta cttcagttgt
    16201 atttcgtttg gcgctccatg cccgaaacca aaggcgtttc gttggaagaa ttggaagaac
    16261 gattggttcg caagtagaga ccttgcatgc aacatctgga agaacgattg gtacgcaaag
    16321 tgcagtaaat caatattttg gcagccgccc gcactaccgg tagagctgtt ggctttatct
    16381 ccgcaatgac cggctaaaag ccaacagctc tacacctgcc gcgggcgccg ggccttccgt
    16441 tcttatagcc tcctaaagtc ggctatacca ctccagtccc tgctgcgcca gccgtcacac
    16501 ccgctacccc tcctcctcct cctcatattc caaaatatcc cccggctggc aatccaatgc
    16561 cttgcagatg gcttccagcg tgctgaaacg gacggccttg gctttgccgg ttttcagaat
    16621 agacaggttg gacagcgtga ggtccacttt ttccgacagc tcgttgagcg acattttccg
    16681 ccgggccatc atgacgtcta agtttacgat gatcggcatg gctcagatgg ttagttcgtt
    16741 ttcagatttc agttccactc ctttttcaaa aacaaccgac agcaaaaaga tgatgccggc
    16801 ccaaaacagc cactctcccg cagcccagtc cgaaggaggc agttccacac ccctgtgcat
    16861 cagccattta ctgtatccgg cgcccgccag cgacaacatg gccgcactca atgcagtatc
    16921 gctcattttg gagagtagct tcacatggcg ctcattgaaa ggattttcta ccttgaatat
    16981 cagcgacata tcaactgccc gccaggccaa tgccaccttg gtacaggcca gcgcgatcac
    17041 taaggagacc atgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca cagcaaggct caccgcaaag
    17161 gagatgatca aggcgccggc tttgatgcac aaaccgatca gtacgatcca aaaggccacg
    17221 ttcatggccc gaagaatgaa atgtgttgtc gttttcatgg ttgtcagtga ttgaatgatg
    17281 gggcaaatat caatgaatat ttattgataa acaataaatt ttgattattt attaaaaaat
    17341 accgcaccaa atgcctccct tgttggacga acggtgaaaa aggcgctcct ttccctgctt
    17401 tttgcgaaag cggcctgctg ctcaaagcgc gcattaaaaa agttctacct cttatttcca
    17461 caaaacgcta ccttagcatc aaaatagatc aaccatgtcc agaacagact atctgacatt
    17521 ggccattgtg gccgtgtgca ttgccgccct cgcgtttctc atttaccgca tggctaccat
    17581 agacgctgat ccggcagcta cagaccccac cgaacaagtg ctggacgagc cggcttccga
    17641 tccgaccatg accgatgaaa ataccgccgg cactgaatac ggcgatgaga cgcccgcacc
    17701 tgactactcg cgcgatgccg atgaaatgga cgacgcaaaa gtggaatctg tggannnnnn
    17761 nnnncccgcc gctaaaaaag accctgccgc cacaaagcct gctgaaaaac ccgcgccacg
    17821 ggccgaaccc gcgccgcctg ccgaggaacc tgcccctgcg cctgcatcct ctgcatcagg
    17881 cagttacatg gtgttggccg gttctttccg cgagatggcc aacgcggagg ctatggtggc
    17941 caaagtgaaa aaattaggct acgaaagcgc ttcagtggaa aaatttgacc ggggagcttt
    18001 tgccgtcgct ttggtggatc gcttcgattc ttactctgaa gccaacgcct tggtcaagga
    18061 attggccaaa aagggcgtgg aggctacggt gtataagaag aagtagcgcg tgagtggcct
    18121 gttccagaca ttgcgatgtt tttttaacat cgcaatgtct ggaacaggcg tggctgctca
    18181 ccatacccct ttcttgatag ccaaacactc caacacatca ggcaggctgt ccctgttgtt
    18241 tttaacaaaa ttgacaaatt ctactgactc ctcatttctc ataatagtaa accaccttct
    18301 ttcgcccaat agcatttcta ttttctcagg ggcttgggga tcgaaagcag ccccagacca
    18361 gaggctgttg ctaaggtccc tttcatttaa tatcacgaag aagtaaaagg cgattgtatc
    18421 atttttgact tcaaaaagca ttggtcggaa ttccaattgc ctgatgctgc cggcctgttg
    18481 aagaagagaa tccctttgaa tttcagcaaa attgaattca tataaccgcc agatagcatc
    18541 ttcgtagtat gggctatatt tcttttcata ggggtagtcc ttagcgcaaa ggctgtaatc
    18601 gttcatgcca catccactaa gaatgcaaca tacagccact gcataaccaa ctatacgatt
    18661 catactcctt tgtttatagt aaaattgatt ctgacccagc nnnnnnnnnn nnnnnnnagc
    18721 cgtcttgtaa aaaaaataat cgctcgtcct gtgtcaccca gaacgggacg cttatctggc
    18781 cgtcttatca aacccactct ttggctcatt gcggccgcgc ttgttcagcc gtggctccgg
    18841 ggggcgctaa aataaagagg caacccgaag gctgcctctc cttattatat atatgtcaac
    18901 gccggatcaa taccccgtat tctgttgcag attcgggttg gcgttgatgt ctgcgctcgg
    18961 aatggggaac acattgcgat aaaccggaac gacagcaccg tctttgacgc cgcccttcca
    19021 ctgccagagg taatccgact gactaaagcg gttgaaacga atgaggtcgg tgcggcggtg
    19081 gcactcccag tacagctcgc gggcgcgctc gtccaaaatc aaatccagat tcagttggct
    19141 gtccgtcacg ctgccggcta cgcttttgaa agcgcgggtg cgaacggcgt tcacatattc
    19201 cagcgccttc gaccggctgc cgccgctgcg cagcaaagcc tcggcgccca tcaggtaggc
    19261 atcggcgagg cggaacatcg ggaagtccgt gtccgggaag gtggcattgg agccttgcgc
    19321 gccggtggaa gtcacgtttt taaatttctt cacggcgatg ccctgcgtga acagcgtcac
    19381 atcgactata tccaaagttt ggcccggacg gtggaaaatg ccccggcggt cgaaggaggt
    19441 agaattgatc tggtaggtgt agtccggctg gttgatgaac aacacgatgc ggtagctgcc
    19501 cgatcccgga atccggttga tggtggcgcc gccctgagag aggaggccgt tgccggcatt
    19561 gtcgcccaga ttgacggtcg ggtcgctgct gacgaatttg aaccctgcgg ccgtcatgtt
    19621 caaattggct actacggctc tcagtgtcgg gtcccaggtc atgggtacag gctgaccgga
    19681 gctgacgccg gggccttcca cgaaccagtc gcggcgaacg atctggtagc tcagttgcgt
    19741 ccagtttact cggatgtagt gcaggcccgc cgttggtaca gtgatgggcg cgccgttgag
    19801 ttcgagggtg ccgtcgccgt tggtatcgcc gtaaatcgga gcggagttgg atgggatgcg
    19861 ggtgaagcgg atggtggtat tagcatccgg gaagtatttg tagccatcaa aaacgttgtt
    19921 gcgcaagggc gaagagagcg aattggacgt ctcggtgggg ttgttgccct ggtgcgaacc
    19981 cggcacatac actttggggt aggtggccgt ttcgccggcg ttgggagagg ctataagacc
    20041 accttccgtt tccgggaatt tctcaatgaa ctgccgcgta gcgcgggtac cgccccagcc
    20101 gccgccgacg ccctgctcag aggggttgat gttgccaccg atggccgcat tgatgatgaa
    20161 cgtcataccg ccccaagtgc gggtgttgat gccgtcaaag gtgatgggga agatgatttc
    20221 gttggagcgg tggttgtcgg ccagaaacaa gtgttggtac tccggttcca gcgtgtagcc
    20281 ggcagtgatg accttgtcac aatagttggc acagtcggca aaacgagcct gcccggtgta
    20341 aacattggcg ttgaggtaca atttggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnngcatcct catctgttac aaaaggcaca ttgcggaaca aatccaaggc atgccagtag
    20581 ctgagtgctc gcagaaaacg ggcctcggca cggtaggtag caatatcggt gcgcaattga
    20641 ccgctcacgc cccggctgtc caatcgggcg tcggtggttt cgcgcagaaa ctcattacac
    20701 acagaaatct gataaaaaat gcggctgtaa aaagcgaaga tgaagccgtc gccgggcgtc
    20761 caggtttgtt tgtgaaaatc ctggatggtc tggtcgttcc agcctatgat ggcctcatcg
    20821 gtagtgagtt actggtggta ccagtagccg cggaggtatt ggccgaaacc ttcgtcaata
    20881 ccggaaatat cggcctggcc tgcagggcct tcctgacctg aaagagcaaa gcctgcatag
    20941 agcttggcca agacttgctt ataaactgcc ggatcgctat acaccgtgcc ggaggtgatt
    21001 tcgagcgggt ccaaaggcac cgtgtctaag tctttgaaac agccggtcgc tgcgaaaaac
    21061 atggcaatca gcagcgcaaa aattttgata tttttcattg ttacagagct tttaatggat
    21121 tagaaattaa cactcacacc caagaggaat gttcgcggac gcggatacac gttattgtca
    21181 atgccggtgc ctaattccgg atccaagccg ttgtaggagg tcagcaccag cggattttgg
    21241 acagtggcaa aagccctgaa attctctccg atgagcttgt cgaaagtata acctacggtg
    21301 atgtgatcca atcggaagaa agaagcattg gtgacaaagt gatcgctgaa agtagccgaa
    21361 gcctgtgtga ggatgttgtt gtcaacagcc gagcggtgta tgttttgcag atacagcgtc
    21421 gggtggaaaa gcctttggag gtagcccatg tcggtagcca cgttgttgta aacaaagttg
    21481 ccgatgttgg cgcggcccgc aaaggagaag tccaatttgc cgatggtcat gttgctggta
    21541 atgccgatga gatagtcggc ggcgggcttt tcgtagcggt acttatcttc gtcgtttacc
    21601 acgccgtcgt tgttgagatc ggcatattgg ccgggcaaaa tgctgccgtt ttcatcgtac
    21661 aattgcttga aaacatagaa cgagcgcggc gcgtatccca cactgtgaat ctggatatta
    21721 ctgcccacgc cgcctgcaat gccgcccgtg aagacgcctt tgtagtcggg gtcgtcggtg
    21781 gcggtcagct tcgtgatttc gttgtagttg taggcaacgt tggcggcaat gtcccagcgc
    21841 actttggaag tagaaaccgg agatacattc aacgacaact caacgcccct gttgaccatg
    21901 ctgccgatat tggtattgat aaaattggtc aggttggtac ctgccggtac ggggatgaaa
    21961 ttgagcaggt ctttggtatt gcgttggtat aaatcaagcg aacccgacag gcggcctttg
    22021 acgatggaga agtcgagcgc caggttggta gtggatgttt cttcccattt gatgttgcgg
    22081 tcgtaaccat tgggacggac cgtattgatg aagaagtcgc cgaactggta gcgggcattg
    22141 tccaaactaa actgataacg agcctgatag atgtacgggc ttccaatatc ttgctgcccg
    22201 gtgacgcccc agcctgcgcg cagtttgatg ttgttgaagt attgattgtc gttttcgatg
    22261 atcttcacgg cagcggccac agcggggaac aagccccagc ggttttcggg cgcaaagcgg
    22321 gaggtggcgt cgccgcgcat ggtgagcgtc agcaagaagc ggttgttgaa gctgtagttg
    22381 gcccgtccga aaagcgatac caaataaagc tcattggcca cattgttgcg ttccaccgtt
    22441 tcggaaggcg tattggcggc gttggaattg cggaaagagt tttcgttgta gaaatgctgc
    22501 caggagtaac cggccatgaa gtccagattg tgtttggctc ccagatcggc tttgtagctg
    22561 agataaaact ccagcagttc attggtgcgt gtctggctgt atgtattgtc tctgccgccg
    22621 ccgttgatgg ggtcgaaaga gaacgccacc acgttgcctc cgggaatttt cagcataccc
    22681 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggatc
    22921 gaaactgagg gccgatccga tggcgccgcg ctcggcaaac tggtttttgg tgagcatccc
    22981 tttgaagcct acattgagct gaagggtatt gttcagaaag cccggcgaga gattgacgcc
    23041 tgcggtgtag cgattgaata cgtcgttttt gagcaaaccc tctttcatgg tatagcccaa
    23101 agacacccgg taaggcacgg cgcccactgc tccggtagcg cctatgctgt ggtcggtagc
    23161 catggcagtt tgataaattt cagactgcca gtcggtactc gcgtctccca tcagacccac
    23221 ctgatcagga tattgttctg ccatcaccgc tctgaattcg tcggccgtca gcacgtccac
    23281 gcgattgtag gtgttgccca tagaaacgtt gccggagtag ttaatgcgca tttttgcgcc
    23341 cgcagctcct tttttggtcg taataaggat aacgccgcct gctgcccggc tgccgtaaat
    23401 ggctgtggca gaggcatctt tgagcacggt gatggactcg atgtcgttgg ggttgatgat
    23461 gttgagcggg ttgcggttgc ccgatacgcc gccgttgtcg agcggtatgc cgtccacgac
    23521 aatgagcgga tcgttgcccg cactcagtga ggattagacc cgnnnnnnnn nntgcggatg
    23581 cgcgcgccgt cgtcggggcc gcccccggtg gtgatggcca cgccgggtac cttgcctgcc
    23641 agcaattcct gcgggctggt gatggcgccc cggttgaaat tggcggagct gacggtttgc
    23701 aaagagccgg tgacgtcttc ccgcttgacg gtgccgtagc cgatgacaac tacttcttcg
    23761 agcacctggc cggcctcgag cgaaatgtcg aatttggtct ggctgccgat gggaacgtca
    23821 atagaggcat agcccgtgta gctgatgcgc agggctgccg tcgtttcggg tacgctcagt
    23881 tcgaagctgc cgtcaaagtc ggtgatggtg ccgcgtgagt agttgtcgcg atccaccaca
    23941 gtggcgccga tgagcgggtc gccggtgctt ttgtctttca ccgttcctgt aatcgtcttc
    24001 tgcgcgagca gcaagcccgg cagcagtacg cacaatgcga gggtcgctat ttgtaagacc
    24061 ctgtgtacaa aattggcatt catgaaaggt tcattttgat tgttaagctt gattagattg
    24121 cacaaaacta taaaaaaacg cgctggcttt acgtcggatt tacaataata aatttttcag
    24181 ggcggagctt ttctccggca gcctgctgcg gtttcacaat tatgttttcg tcagtacttt
    24241 tatttgattc gcttaatttt gaaccggctg gattcttcgt ttttttatcg tcatttttcg
    24301 cgcaaaatat aggtggcatg aacaagatac attagctttg cgtcactcat acaataattt
    24361 tcatcactaa aattgagcat aatcatgttg agacttctat ctactttgct tttctgtgct
    24421 gccgccctca tcggctacag ccagattact actgtcggta tcatcggcac ggccacgcca
    24481 aatggctggg acagcgatac cgatatggta caagacgccg cggacaccgc taaatggacg
    24541 ctgaccatca cgctcgttca gggcgaggcc aaattccgcg ccaacaacgg ctgggatgtg
    24601 aactggggcg actccgcgtt tcccatcggt acaggtacac agggtggccc gaatattccg
    24661 gtacccgcgg gcacttacaa catttctttc aactccgtaa ccggcgccta tttctttgac
    24721 ctcgtttccg acatcggtat catcggcagc gccacgcctt tcggctggga tcgcgaagta
    24781 tttatgttcc agagcgaagc ggataccaat cagtattctc tgaccctcaa tctggcgcag
    24841 ggcgaggcca aattccgcgc caacggcaac tgggatgtga attggggcgc agcagatttc
    24901 cccgccggca tcggtacacc gggcggcccg aacattccga ttgcacaggc aggcaaatac
    24961 ctcatcgagt ttaataaggc tacaggcgct tacaacttca ccgagcaggt agaatttact
    25021 tctatcggag tcatcggcag cgctacggcc ggcggctggg acgctgaaac ggcccttacc
    25081 cgagacagtg gcaatcccga tctttggaaa ggtacggtta cacttacaga aggtgaactg
    25141 aaattccgcg ccaacaacgc ctgggccatc aactggggcg gcggggagtt tcccagcgac
    25201 acagccactg ttaacggtga caatattgcc gtgcccactg ccggcgatta tctggtttcc
    25261 ttcaatacca gaaccttgat ttacaccttc ttgctcattg gcaattacac ttctgtcggc
    25321 atcatcggcg acgctacgcc cggcggatgg gataacgata ccgacatgac gcaagacccc
    25381 gatgatcgtt ctatctggaa gttgcgcatc atacttactg cgggtgaggc caaattccgt
    25441 gccgacaacg attgggccgt caactggggc ggcggtacgt cccccaacgg tacggctact
    25501 caagacggcc cgaacattcc tgttactgcc ggcgaatata aagttacatt caattctact
    25561 acaggagctt acacctttga agaaatcatc gagtttgctc aactcagcct agtgggcagg
    25621 tccggtccgt tccaaaactg gcccggcggc ggtccctgtg ccgatatcaa ctgcgacact
    25681 taccttgaga aaaacccgaa cgacggcaac ctctggagcg ctaccggcgt gacgctcacc
    25741 acgtctgctg atgagcagga tggcggtatc aagttccgcg ccaacaccga ctgggcagtg
    25801 aactggggtt ctgccgcctt ccctgccggt accggtactc aaannnnnnn nnnnnnnnna
    25861 acgtagcttt caatgcagta accggcgaat atgctttcag tccggccagc tctacccgcg
    25921 aagacctgct gtggccttcg gctattcagt tgttccccaa cccggcttct gagtttgtta
    25981 atgtcaatat tgattccgac aaattggcca acaacatcca aatgtccgtg tatgacaact
    26041 ttggtcgtca ggtgatgcgc cagacgtttg actctaaagc caacatccgc ctgaacgtag
    26101 cttcgcttcc ggccggcatg tacacactga gcattaccga cggcaaatac atcgttggta
    26161 agcaattggt ggtgacgcgt tgattttgag actgtgatag tgaaggggtg attttataat
    26221 tataccctca ctctgtaaga aaaaatggnn nnnnnnnnca caaagccgcc tcgatgtatt
    26281 tcggggcggc tttgttgttt catcgttttc gcaaaatcat catatcttta gccccgtaaa
    26341 tcaaagctgt acagatgctg ctcattcagg atattctcat cagcgacgac gtagtgaaat
    26401 cgcattttgt ttgcaaactc gatgcctgca aaggcgcctg ctgctgggaa ggcgactacg
    26461 gcgcgcccct cgaagtgaac gaactgccca ttctggagcg aattttccca caggtacgcc
    26521 cgtttttaac gcccgaaggc attgcagtta ttgaagaaga ggggactgcc gtttacaacg
    26581 aaaaccacga gacgtatgcc acgcctttgg tcaacaacgg cccctgcgct tatatgaccc
    26641 gcgacgaaaa aggcatagcg cactgcggca tagaacaggc atggaaagcg ggcgctgttg
    26701 attttcaaaa accgatttcc tgtcatttgt atcctattcg ggtttctgtg tcggacattc
    26761 cgggattcga ggcgctcaat tacgatcagt gggatatttg caatgcggcc tgctcttttg
    26821 gcgcacagtt gcaagttcct atttatcagt ttgttaaaga tgcgctggtg cgcaagtacg
    26881 gccaggaatt ttatgatgaa ctgcacgctg cggcgcaagg gtatgagtga ttcaggggtg
    26941 agtgtgtgac caacccgaaa ccttaaaccc gaaacattga actcgaaact cgaaacaaac
    27001 aatgcttatg aaactgcact ttctcctttt cctcctctgc tttgccggca cactctccgc
    27061 ccaaagcacc ggtcgctgga ttaccctgtt caacggtaaa aacttggacg gctggacggc
    27121 caaaatttcc ggttacgagg cgggcgataa ctttgccaat acttaccgcg tcgaaaacgg
    27181 cgtcatcaaa acagcctacg acggatatga tgacaacttc aacaaccgat tcggcctgct
    27241 tttttacgaa aagcctttta gtcattaccg tcttaaactc aaatatcgtt tcaccggcga
    27301 acaatgcaaa ggcgtggcgg agtgggcctg gcgcaacagc ggcgtgatga ttcactctca
    27361 atccccgcaa agcatgcccc gcgatcagga ttttcctatc tcgctggagg tgcagtttct
    27421 tggcggcgac ggcacgaacg agcggcccac gggcaatctc tgtacgcccg gcacgcatgt
    27481 ggtcatcaat ggaaaattgg aagaacagca ctgcatcacg gccaatgcgc ccaccattcc
    27541 cggcgaccgc tgggttcgcg cagaagtgct ggctctgggc gattcgctga ttcagcacat
    27601 catcgaaggc aaggtggtca tcagctattc caagcctgtc atcggcggca cagtggtgag
    27661 caaatacgat cccgctgcca aaaaagacgg tatgccgttg acaggaggct acattgcttt
    27721 gcagagcgaa agccatccca ttgaattcaa gcaaatcaaa ctgatgatat tggacgaaaa
    27781 cggacgtccg gttcgcaccc aaaagcgcaa gtgatttcac atgagtcgcg tactttttta
    27841 cacaatcgtg aagcctttgt cgcggctgcc gctgccgctg ctctatctgt tttcagacct
    27901 gatgtatgtg atcgtttatc gtatggtcgg ctttcgcaaa aaagtagtgt ggaacaaact
    27961 ctgcaatccc tttcccgacc aatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn aaagcgtcgt attgacagga gggcactaca acagttggga actgatggct
    28201 atggttttcg atgtgcaggc gccgcatcaa accattgcag tttattctcc gctttccaat
    28261 tcgttttttg acaacatatt gaaggcgtct cgcggtaaat tcggtacaat cttagtgccc
    28321 aaaaacgaca tcggggattt catcaaacaa cagcagggcc aattgaatct gtacattctc
    28381 gttgccgacc aatcccccac ttatgccaag aaggtgtatt ggacgaaatt tctgaatcag
    28441 gaaaccgcca tagctaccgg cccggagaag ttggcaaaat tgttcgatat gccggtcgtg
    28501 ttttgccatg tttacaaact gcgcaggggc tactatgaag tgattttgga ggatgtagaa
    28561 cctcgcccga aggaggccct gcccggcagc atcacggaag ggcatgtgcg gctgctcgaa
    28621 aaggacatca tgacagcccc gcaatactgg ctctggacgc accggcgctg gaaacgccaa
    28681 aaataaattt taaaaaaatt attccatcag gcagcgaaca gcagcgaatc tgcatctatg
    28741 ctttcggtga ccagacacac cagttttttt tcctttagaa agtgcgaagg ccgccgacta
    28801 ccttggtcgg cggccttttt ttgtactttg gtctctaaaa ctcataaagc gcgcatcggc
    28861 tgctcaaatc ctgcgggttt caatcggctt cggcacattc tttttgtaca attggtctcc
    28921 cgactcattg acggcattgt gcaagggggc caactcctcg ttcagtttga caatcactgc
    28981 attgtaagcc tccacatctt cgttggtccg cttgtcggct ttgcgaatca catcggtcat
    29041 ggttttgtac ccattaacag ccaggtcttt ataaaatttt gtcagcgcca gcgcggcatc
    29101 gcgataagca gtatcgccgt tgaaatcggg tagcttgctg aaaaaataga ggtgatcgtc
    29161 gcagatcaca tccaattctt tgcgataacg gtccatcttt ccggcatttt gctccttgag
    29221 cgcatccgaa aattcagcat cttttttaga tagtttgaac acccgcaaaa acaccgcccg
    29281 gtagtagtca ttgagttgcg acaaggtgcc ccctccggcg tcttcttcgc cttcctctat
    29341 ctggatttta tactttctgg caaaagctac ctgtgccgcc gaagcccgtt tcgaggcagc
    29401 gtctatcttt ttttctgcct ctgcattggc tttgaagtag ttttccatgg caatgtacga
    29461 ctcggtactg gtgcgtctca acaggtttac ttgctggaat tccactttga acgaggccag
    29521 atacgcctca aataccgctg caatttcgtc ccgcagtttg gagtcgcctt cataggccgg
    29581 catggtccga acgttgatga gcgactgctg gatttgctga atcaaggcca tgcgtttttg
    29641 ctctatcagg gaataatcgt cgctgtgtac gctgtactgg aggtagtcca tatttttgtt
    29701 ggataccaga gcgtgttcgt tgaacaaggc tgtgccataa gctaccggcg atgaataacc
    29761 ctgcgcccaa acggcagcgg agcaacacag aaacaggagg agggtgagga gtggttttgt
    29821 cataatggtt gaaattttag tgtgatgttg aatggagtta taaatgattt tgccggctga
    29881 aaattccgac tatttgccaa caacgccaaa cgatttggga gaaaatgaca aaaatcaata
    29941 tttgatccct gccgaattgg ctacttttgg caaacatgtg aatggcgcct atgttcaaac
    30001 tctatttcat caccatcatt gccgtgctct tgctgtgcag cccattgagt gcccaacgcc
    30061 aatattgggc gggcgcaggc ttggaactgg ctgaaacaca gggactcacg ggctgggctt
    30121 tcggcgcgca aaccggcgtc gcatggaggc gcagctacac cggagttttt gttcgtcaga
    30181 ctacggctct ggtgcgggca gagaatgcct ccaatttgcc ttacgatatt tccatgcaat
    30241 acgcgggcct gctgggcgcc tggcatcggc cggccatcgg cgcgctggag gtcggcgtcg
    30301 gttttcagat cggtttgggg cgggctgttc gtcgctggga tagctcgctg ggcttgccca
    30361 acgacaaaga cgccattctg gtagctgtac cgtttgtggg cttggagctt cccctgggcc
    30421 gcaggtttcg catagccgcg cactccggat atcgcttttt cggcgggttg gacggcatag
    30481 atcagtggca tggccgcgat ttttgggctt tggccaattc catccagctt cgggtaaatt
    30541 ttgtagatag aaaatgagct gaacgctgta ctttcgccag aaggaacggt tcaggcagct
    30601 tgtagcccgc ggcttgagac cggccactac caaagatcaa caacgattgc ctatgcctgt
    30661 caaaaccctt ttggccgttc tgttcatcct gtcggggctg aagctgcttg cacaggatca
    30721 gcatttttct cagttttaca accacccctt gcatctgagt ccggcgcttg cgggcgtgtt
    30781 ttccggcgac cagcgtttca gcggcgcctg gcgcagccaa tgggcctcgg tgccggtgcc
    30841 ctacctcact ttttccggcg cttacgatca gaaaatccct ttgcctggcc tcaaaaacgg
    30901 ctgcctcgcg gcaggtatgc tcttcaacta cgaccgcgcg ggcgatgccg gactcaactg
    30961 gcttcaggtg ggattgaaca ccgcttacac gcagcgtctg gcggaagctt tgttcctcac
    31021 tgccggggta gaggcggctg tcggtcagcg ggctttcgat ccgcagcagt tgtacttcga
    31081 cgagcagttc aacggtgata ttttcgaccc atcgctgccc actcaggaga cttttttgca
    31141 aacggctgcc ggctataccg acctctcggc gggtctcaat ttgttggtgc aggcgcctca
    31201 acagcgcagt tttgccctga tcggcgctgc ataccggcac ctcaatcgac ccaccgttcg
    31261 attttaccaa caaagcgccc tgtcattgcg cgctctgacg aatatttacc tctccggcac
    31321 cgtgcagaca tctgaaaaaa tagacgctac ctttgcattg gccgcccgcc tacaaggccc
    31381 ttaccgggaa atagtgagcg gcgcaggcat tcgctactat ttgaaaaaca acgacttaca
    31441 ttctatcgcg gtgggcgccg gggcatcgta ccgctggggc gacgcgctgg tgtcttacgt
    31501 cgaattgtgg aaagacaact ggcgtttggg catcagttac gacatcaata cctcgccgtt
    31561 cagggtggcc acgcagcgca atggcggccc cgaagtgacg ctgcaatatt tcatccacaa
    31621 agtcaaagcg cccgaaactt tcaagatatg tcccattttc tgattcgctg cgccctttgt
    31681 tgcgcccttt tgacggctgc tctggcagaa actcaggcac aaactttcaa ggcttgggaa
    31741 aaagccggcg ataaagcatt gaaaaataag gatgtaaatg cagccatgca ccactatgcc
    31801 cgtgccttgg gtctgaatcc cgctcacgtt ggtctggggt acaaatatgc aggcttggct
    31861 caacgcgccc atgcctacga agccgcccgc gattattacc tcaaggtgac ggcatctccg
    31921 gaggccgggg tgtatccgca ggcgctgttt cagttgggaa tgatggaaaa aacgctgggc
    31981 cgatacgatc aggcggcttc gcattttcgg gatttcattg accggtacgg cgcttccgac
    32041 cccgcccgga aagcgcaggc agaaagccag ttggaacagt gccgccgggc tgctgaaatg
    32101 tcggcgcaac cgcccactgc ggagctcgtc aggttgggaa aaagcgtcaa ctcggcttac
    32161 accgaattcg gcccatatcc ggcgggcgac acgctctact tctcctcgct gcgcttcgac
    32221 aatcccaaag acaaaaacct gccgccgcgt atggtgtcca aggtgatgag ttcgctgcgt
    32281 tcgggcgccg gccgggcgct gaaggatgga tttaatgaag acacccgtca cacggcgcat
    32341 tttgcccgca gccacgacgg tcgtcgggtg tattttaccc gctgcgatta tgtgaacgcc
    32401 tccgaaatcc gctgtgccct ctattaccgc gaacccgacc gccggggccg cctgcgcacc
    32461 gaaatccgtc tgcccgatcc gctccatctg cctggattta cggccacaca acccgccgtg
    32521 gggtacgatt cccttgctca aaaagagtgg ctgttttttg tatccgacag gccgggcggc
    32581 gccggagggc ttgacatctg ggcgagtgag gtacttgcaa aaggcgcgtt cggaaccccg
    32641 gagccgttgc ggaacatcaa caccccggca gacgatatta ccccgttctt tcatgcagct
    32701 tccaaaacgc tgtatttcag tacagaaggg cggttcagtc tgggtggttt cgacatttat
    32761 tcctccccaa aaacagcagg cggctgggct gaaccggtac acggcggcct accgctcaac
    32821 agcagtttca acgatattta ccctgtattg gagcaagacg gcgtgcgggg ctacctggcg
    32881 tccaaccggc cgggatcgct gtatctggat gcctccaaca agacttgttg caacgatatt
    32941 taccagtttc gcctcctgcc gccgcccgca gacagcacca ttccgatgga gcaagcattg
    33001 attcccccac ctgtagagac gcctctcatt gccgaggagg aaaagctgcc gcaaaccctc
    33061 gaagattttc tgcccctggc tttgtatttt gataatgacg aacccgacaa gcgtacccgt
    33121 cgaaccacta cccaacaaac ctacggcggc agttttgaaa aatattacgt ccgaaaacag
    33181 gagtacatgc gccagtacgc gggcgagctg aaaggcgatg aacagcaaga cgctgaagat
    33241 cgtatggaag atttttttga gaacgacatt cgccggggac acgacttttt aattcgtttt
    33301 tccgaaatcc tgctgactcg cttgcaaaag ggcgatcagg tggagatttt tatcaaaggt
    33361 tttaccagtc cgagggccga aacagattac aaccttaatt tgggcaagcg ccgcatcagc
    33421 agcgtaagga atcattttga ggaatggtct gacggccagt tgaagcccta tcttcaatcc
    33481 ggtcagttgg tcataacaga aaccagtttt ggt
//
LOCUS       DAORLF010000005        33174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_700_length_33174_cov_2.013225, whole genome shotgun sequence.
ACCESSION   DAORLF010000005 DAORLF010000000
VERSION     DAORLF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 33174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33174
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_33174_cov_2.013225"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>219
                     /locus_tag="PK198_01160"
     CDS             <1..>219
                     /locus_tag="PK198_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRF37370.1"
                     /translation="FMREEEKLAHDVYQALLGRWNVEVFSNISKSEQRHTDAILCLLQ
                     KYNLTDPVGSNGPGVFQNADLQALYTVLV"
     assembly_gap    220..304
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <305..793
                     /locus_tag="PK198_01165"
     CDS             <305..793
                     /locus_tag="PK198_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRF37371.1"
                     /translation="ATPDINNADILAVFGELTKGSRNHLRAFTRQLVQLGATYTPQYI
                     SAETYAQIIASEHERGGSACVSNCNGNQQGNGCNNNRRGRQGGNGNCDHNGSGNGGNG
                     CNGGNGNGGNGTGGNGNGGNGNGGNGTGGNGNGGNGNGGNGNGGNGNGGNGNGGNGNG
                     GN"
     gene            884..1483
                     /locus_tag="PK198_01170"
     CDS             884..1483
                     /locus_tag="PK198_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF37372.1"
                     /translation="MLISKKICDELDDLQIIRKALAETDYFSCLYERYEAQLLRYVQR
                     VAALSEEEAQDVLQEAFIKIWRHLNAFDPQLRLSSWMYRIVHNEAVSALRKKTSFGKD
                     QQLVIDESLQSRLAAEWPDLGYNDEIEDREHAIRTALSELALPHREVLVLRFMEDMSY
                     EEISDVLKIPEGTVATRLNRAKKALAAAFASYSSNTKRP"
     gene            1486..2196
                     /locus_tag="PK198_01175"
     CDS             1486..2196
                     /locus_tag="PK198_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37373.1"
                     /translation="MKNSERLIQAIKERRIAPVPKAFFTGRELLLWLVFALSALLGAA
                     AFSVILFSIQQTDFYLLQHSGHSRLELLLSWLPFVWMAFLFVFLGLSMFSFRNSRRGY
                     KWRLTHLAAYSFGFSVLLGTAFFLAGGAARLERAFDVRVSIYQSMEEKKIKVWSMPEQ
                     GYLSGVIETVDGEEMTIRDFDGKIWAIDISNAFVAPVLALDAGEQVKLTGRMTSEGRF
                     EADGVRPWGGPGFRHQKK"
     gene            2232..2639
                     /locus_tag="PK198_01180"
     CDS             2232..2639
                     /locus_tag="PK198_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37374.1"
                     /translation="MNRFCGILLFSAYAFAVLVPPAFKQELGKLDHLLNHYQEHRAET
                     PDMSLIAFLKMHYGEQFATHSADHSHNNLPGKTSQDHTHAVACGCSVFELICHFDWSL
                     RRPAPQQHTLFPTEINLLSTLHASGIWQPPKHV"
     gene            2698..>5215
                     /locus_tag="PK198_01185"
     CDS             2698..>5215
                     /locus_tag="PK198_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRF37375.1"
                     /translation="MLDAIIRLSVNNKLIVGLFTAALIFAGVYAFVQLPIDAIPDITN
                     NQVQVLTVSPTLATQEVEQFVTAPIEHAVRSIPGVVELRSISRFGLSVITVVFEERIN
                     PYLARQLISEKLKVAEEMIPPGFGTPEMAPISTGLGEILHYRVEPLPGYENRYTATDL
                     RTIQDWIVKRQLSGIPGVVEINSFGGFLKQYEVALSPERLRSFGIGIQEVFQALENAN
                     ENTGGAYIEHTNNAYFIRSEGLVQSLDDIRQIVVRDNAGAPIKIGDIGEARFGHAVRY
                     GAVSHNDEGEIVLGIVMMLKGENAARVIRRVKERLEVVKKSLPEGVTVTPFLDRENLV
                     NRTIATVRNNLTEGALIVIFVLVLLLGNWRAGLIIASVIPLSMLFALWMMHLFGVSAN
                     LMSLGAIDFGLIVDGAVIIVEATLHFLAVRVLQKQGEGATRFTRAEMDENVSTAASRV
                     MRSSVFGIIIILIVYLPILSLSGVEGKMFRPMAQTVSFALIGALILSLTYVPAVSALV
                     LSRRIKTTHTFSDRLMEWLKKLYAPSLELALRFPKFIIVSTLLVFSLSVGLFLRMGGE
                     FIPTLDEGDIMMHGFLRPGTSLTQTLESHRLAQKIILDNFPDEVEQVISKIGSAEIPT
                     DPMAIETADNIILLRDKSLWKRAATKAELVELIEAKVHEIPGMAFEFTQPIKMRFDEM
                     MTGVRSDIAVKIFGENLDSLARAGHRVERLLSAIEGVEDVKTEQIEGLPQIVVKFNYG
                     QIARHGLHIRDVNETIRAAFAGATAGTVFENERRFDLVVRLNTLHRSRIEDVLQLPLS
                     TPSGALIPLSEVAEVRFLPGAAQISRDDAQRRIVISANVRG"
     assembly_gap    5216..5331
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5332..>5573
                     /locus_tag="PK198_01190"
     CDS             <5332..>5573
                     /locus_tag="PK198_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF37376.1"
                     /translation="SIAVPVALALIFMLLYFAFNSLKEGLLIFTAIPMAAVGGVLALL
                     LRGMPFSISAGVGFIALFGIAVLNGIVMIAYFNPLE"
     assembly_gap    5574..5595
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5658..5822
                     /locus_tag="PK198_01195"
     CDS             5658..5822
                     /locus_tag="PK198_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF37377.1"
                     /translation="MGLWRSGSLSHGAGAEVQRPLATVVIGGLLTSTLLTLVVLPVLY
                     AMFFKSNQEQ"
     assembly_gap    5952..6154
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6155..7168
                     /locus_tag="PK198_01200"
     CDS             <6155..7168
                     /locus_tag="PK198_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF37378.1"
                     /translation="PADLFHNIAADPDYGMFGTTAFGFNQAFPARKMTQARRLYYQRR
                     EAAAAAGFMLTRRQVARSVSELYQHIGFIQSEAGLYRRLDSLYQSVAAIAQLRYTAGE
                     VSKAEQLALEDKAAQMRLALQTTGHEIEFDRVVLGQVLGIPGQVFPVSGPFQPMSFSL
                     ADTALVENSARSVLYRTSIAISEAEQEVVAAQRAPVFSGGLMVQYLPNGRVLPGWQLG
                     LQIPLITKSLRADQAAAALGVQSAAADYRADLLEQRTQLAHLLHEQEKYQIRLEYYTA
                     KGKALADELLRMAVANYRAGEIGFVELTQFAEQAAAIELDYLENLLGLNMAIIQLRTL
                     TGQ"
     gene            7401..8561
                     /locus_tag="PK198_01205"
     CDS             7401..8561
                     /locus_tag="PK198_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF37379.1"
                     /translation="MKKYILLSGLALIAAACGTKNAEPVSEAPAAEEIHERISLSAEQ
                     FRQAGIQTGTFSYRTMQEYFPAAAELYLGAEHTASVSTITEGIVSELRVTLNQTLRKG
                     DVVAVLHKPGLLDWQEEYLERKDRIAFLKMELERYKTLAADNATATKNLQKAEADLRE
                     AQTSQTLLAAKLRQYQIDPELVTTSNLKTQIMLRAPISGTVTKIHSGLGASLAPGAAL
                     VDMADYSRVQPVVYIFEKDIHRVKVGARVLLFFAADPGRTFPAVITNMDGAVDRERRA
                     LRAFARFEQSPDKLAAGAYMDAKIAPSAGSETQALPEAAVVRESDGQYIFILELERAD
                     GVIFHKVAVQTGAVEGGFVAVTPLEQLPKDAKIVLQGAYYVSAQGAGIEVEE"
     gene            8656..9561
                     /locus_tag="PK198_01210"
     CDS             8656..9561
                     /locus_tag="PK198_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37380.1"
                     /translation="MKKYSLLFLPCLLLSVCLTAQPCPDKPYIKAPELSDAVRKTFEA
                     QLAQAQTTYEARPDDADALIWMGRRTAYLGRYMEAIELFGKGVEKHPADARFLRHRGH
                     RYLTVRCFDAAIEDFQAAARLTRRLPDEVEPDGLPNARNIPTSTLQSNIWYHLALAHY
                     LKGDFKKALPAWKRCVELSQNPDGLVSSTHWYYMTARRLGLQRKAEKALQKVGKDLDI
                     IENQDYYTLIQLYQGRIGEGDVQKMLRTTDNALSNASLGYGYGNWLWYNGRQQEARQV
                     FAQITQGSSWAAFGFIAAEAELSRK"
     gene            complement(9676..10281)
                     /locus_tag="PK198_01215"
     CDS             complement(9676..10281)
                     /locus_tag="PK198_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRF37381.1"
                     /translation="MDTILQPESINAKEAATQYPVHEFIRQRWSARSFADRPIEHDTL
                     MTLLEAASWASSSMNEQPWVYLYAHKGEPVFQQMYDCLLPGNRWADGAPVLLLSLARK
                     NFASNGKHNRHALHDVGAANTTLLLQAAAMDIFGHQMGGFDMQKTIETFQIPDDMEAV
                     CFISIGYLDEPEKLEEPFRTRELTPRKRKELAEFAFAGKMI"
     gene            complement(10410..10718)
                     /locus_tag="PK198_01220"
     CDS             complement(10410..10718)
                     /locus_tag="PK198_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37382.1"
                     /translation="MIRYLYILKIKQIRNEKRSGMSAAGAMALGAALSVLFFTGMPTG
                     SAGFSQVRFFATGFACFFFHRGGTAAARHFAVGKMALQRKTEDLHAGRQRHEERDKKQ
                     "
     gene            10986..>13282
                     /locus_tag="PK198_01225"
     CDS             10986..>13282
                     /locus_tag="PK198_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37383.1"
                     /translation="MRIIIFRLSAILLLFTSLSAAAQNQDHNTLKIWVNGACGMCEDR
                     IQKTALRTPGVHTAVWDEHTKMLTVTTGAKFKEDQLHQNIAQVGHDTEKVKAPDDVYE
                     NLHACCHYDRSDVPPSQKSKTIRGKVLEELKKGEESPLIGAVVHWLGTSQGSTTDADG
                     HFELEPHPGSNRLVVSYIGYRPDTVEVKDGFDLDIVLSNTHTLKEVEVFYRKKATEVS
                     FFSPIQVQVISEKELLKAACCNLSESFETTPSIDVNFTDAVTGTRQIQLLGLAGPNIQ
                     ITRESMPDVRGLSALYGMNYTAGSWVEGIQLSMGAGSVVNGFEAIAGQINVELRKPET
                     SDLMYLNLYAATMGRYEGNANFSYKINEQWSGGVLLHGKSQQREVDHNHDGFLDNPLS
                     DALIAIKRWGFQGKKGWEAQFGLKGALLNDKSGATGFHEDVELHTGHWWGASVATKRA
                     EVWAKIGRVLPNKPHTTYGLQLSATRHEQRAYFGLRSLNADQKSAYANFIFQSIIGDT
                     RHTYRTGASFQWDNFEENANNRWYDRNEQIPGAFFEYTYKPDDKLTAVAGIRADYHNN
                     YGLFFTPRLHVSYTPRDLTVFRISAARGQRTASVWAENIGIWASNRNIVLHGDAEGLP
                     YGLRPEIAWNFGLNFTQGFEINHRAATFTAGVYHTNFVNQVVVDFDKGPQELHIGNLD
                     GRSFSNSAMAQLDVELLAGWDLRLAYRFNDARTTFAPGLLERPLLSRHRAFLNTAYET
                     KSGWHFDFTLNWQGARRIPSTASNPA"
     assembly_gap    13283..13302
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13303..13534
                     /locus_tag="PK198_01230"
     CDS             <13303..13534
                     /locus_tag="PK198_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37384.1"
                     /translation="LERSPDYFLANAQISKRWGDKYDLYIGAENIFAYTQHHPIIGAN
                     DPFGPYFDSTLVWGPIFGRNIYVGGRWRIARR"
     gene            13662..15224
                     /locus_tag="PK198_01235"
     CDS             13662..15224
                     /locus_tag="PK198_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF37385.1"
                     /translation="MKRYFNTTGFCNPEKHYMVNPFRDIYDDILRLVEAEQYFLIHAP
                     RQSGKTTFLHSLAHRLNQEGKYISVVGSLEAAGYSSITVADANWVMVKSLYQNAKLFL
                     TEDYVPRNPDHYTKDSSLFQTYLMDWCEELPKPVVLLLDEVDALYDDVLISVLRQLRD
                     GFQRRPKLFPQSIALVGLRDIREYKMRARADNPSIGAGSPFNVKAESFFLPVFSLEEV
                     RGLLQQHTDDTGQVFTEEVLQKLYEYSGGQPWLTNALANQIVVKILKGDYSRAITPDM
                     VEDAKERLIAQRQTHLDSLADKIQEERVRRIVTNIISGGDIQFDGADDNLRYCRDLGI
                     ITAKSPVQFANPIYREIITRIMSAGFQESINQDIAQTSWYLRPDGSLDMDKLLEAFVD
                     FYRWNSESWLERFQYKEAGHQLLLMAFLQRIINGGGRIEREMAIGNGRTDLAVFWKDQ
                     VIPIEIKMHHNARSQPDGLRQLARYMDKLGQKQGYFILFEKKSSEELSWEDRIRREIH
                     EVDGKEVILLGM"
     gene            complement(15248..15448)
                     /locus_tag="PK198_01240"
     CDS             complement(15248..15448)
                     /locus_tag="PK198_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF37386.1"
                     /translation="MKNRVKEERARLNLQQQELAERIGVSRQTIHSIENNRYVPSTVL
                     ALKIAALFQKPVEEIFELEEGD"
     gene            complement(15445..15906)
                     /locus_tag="PK198_01245"
     CDS             complement(15445..15906)
                     /locus_tag="PK198_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37387.1"
                     /translation="MKTMRLLPVTWKWPGAILSILGLALGFAAELGEFSFSWLKFIVR
                     EKNSFLDTAEEDFTNEVALTLTIVGLLILAFTRERDEDERVRLIRLEAFQWSILVNFI
                     IVLIGNWVLYGGSFFWLMTFNLFTPLVVFLLRFYYVLYIRESGAGKLNSIL"
     gene            complement(16028..17203)
                     /locus_tag="PK198_01250"
     CDS             complement(16028..17203)
                     /locus_tag="PK198_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF37388.1"
                     /translation="MKHITRSVWILSLISLLTDTAGEMLYPIMPIYLKTIGFSVVLIG
                     ILEGIAEATAGLSKGYFGKYSDTMGRRAPFVQVGYALSALSKPMMAFFIYPLWIFFAR
                     TIDRLGKGIRTGARDAILSDEATPQTKGKVFGFHRSMDTLGAVLGPLTALLYLHHRPN
                     DYKTLFYIAFLPGLFAILASLLLKDKPTTAAGSRAPVSFFSFLRYWKESPAAYKKLVV
                     GLLAFALFNSSDVFLLLKAKESGLDDTHVIGVYVFYNLIYALCAFPLGALADKIGLKT
                     VFLSGLLVFAAVYFGMAFNTNIYGFLGLFFLYGVYAAATEGISKAWISNISDKKDTAT
                     AIGTFSGLQSICAMMASTLTGFLWFRFGAATAFVATTLVTIITAAYVLTSIPRPSTD"
     gene            complement(17214..18692)
                     /locus_tag="PK198_01255"
     CDS             complement(17214..18692)
                     /locus_tag="PK198_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF37389.1"
                     /translation="MRSYLILLMLVMCSRLASQSMDTIPLSLAQAVEISHSDAPDVAI
                     ARTIYSNNYWRYQSFLANYRPAITFNATLPNLNRSIQPITLPNGQDAFISRSLMTNGA
                     GISLQQGVAATGGRVFMRTNLERIDIFGTDEKPGFISYLSTPVSIGFEQPIFGFNQLK
                     WGKKIEPLRYEESRKRYSENMAETALRTTELFFDVLMAQYSLEAARKDKADADTLLSI
                     SKGRFEVGRIALDELLQIELSAMNAEAAIAQQTLTMQTATERLRDFLGIRTAVVFQLV
                     TPAGIPDFRVDAHKALLQARMNRSDVLAFERRNAEAQMTIAQARAERGLQMNLFGSFG
                     LSQTGPDIETAYTRPLDQEQVVLGITLPIADWGRSRANLEIAQSNQELVEMQVAQERI
                     NFEREILIRVHQFDLVRNQVQLAMAAHDVSKRRLEMSRNRYMIGKILITDLNLALREE
                     AEARRSYVSALRNFWLAYYDLRRITLYDFENDRPLIETPEEK"
     gene            complement(18824..20146)
                     /locus_tag="PK198_01260"
     CDS             complement(18824..20146)
                     /locus_tag="PK198_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF37390.1"
                     /translation="MERWLFNFGLALRGINANKLRSLLTALGIVFGVAAVIAMLSIGS
                     GAKRAILDQMRLIGANNIVLEAIVPDPEKEASSGGSNANGNNKDKRPWSPGLSLADIQ
                     AIRENVPGVESVSPEIVLPTTFVYGGKQEKGRCIGVENTFFYLNRLELGAGKLFHAEH
                     FEAGRPVCIIGKDVQSRFFAGADPMGKKFKCGNTWFTVIGVLQKRNAGKETLDNLGIK
                     DYNTDVFIPIQTALLRFENRAVISKSDLGRGNDEGKSANYHQIDRAVIRMESSKLLPA
                     AADLMARMLKRRHRDVVDYEVEVPELLLQQEQKTQDTFNMVLAAIAAISLLVGGIGIM
                     NIMLASVLERIQEIGVRRALGARQSDIVQQFLLEAVMISLFGGMIGVLLGVLAARIIA
                     ASADIPTIIAPWSVLVSFAVAAAVGLIFGIFPARRAALQDPITALRRE"
     gene            complement(20152..21561)
                     /locus_tag="PK198_01265"
     CDS             complement(20152..21561)
                     /locus_tag="PK198_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF37391.1"
                     /translation="MTRKNIVRIGVAALILTLCGSAIYTFWSKKTNETETAAVLKYTV
                     KKDVFHVRVTATGELSAKRSVKIRAPQGMNAAGIWETTITDLVTEGSLVKEGDYVATL
                     DRTELANKMSTIQTEIEKIQTQLEQARIDTAIELRGIRDELVNLDFNKKEKLLYLDQS
                     RYEPQSVIQTARLDLERVERDFRQLEKKYDLKRQQSVAKIQEINALLRQNQQQLDILT
                     KLSGEFSIHAPKSGMIIYARTWNGKREPGSRISSWDPVVAELPDLTDMISKTYVNEVD
                     VSKVQPGQDVSIKVDAFPDRSYTGKVISVANIGEQIKGYDAKVFEVIVQVSESDSILR
                     PAMTTGNEILTGSYPDKLFIPLEALQSDSVTFVYKEKDGKLVRQEVITGLSNDNQIII
                     EHGLEENDVVWLAPPPDTSELPMIPIAPDIKAAIKKEQEKAAERRRKEAAEKRKTVEG
                     IDVPTSDGGSGGDFIIIME"
     assembly_gap    21980..21994
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21995..23334
                     /gene="dnaA"
                     /locus_tag="PK198_01270"
     CDS             <21995..23334
                     /gene="dnaA"
                     /locus_tag="PK198_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRF37392.1"
                     /translation="KTWFEPIKPVRLQDNALTIQVPNKFFYEWLEEHYVSLLKSSIRN
                     ELGDRGRLEYQILMGTNERPIATAPDKSGPFTPGSFETDAIKNPFVIPGIKKIKVDPQ
                     LNAAYTFESFIEGDCNRLARSAGLAIAKKPGGTAFNPLVVYGATGLGKTHLAQAIGNE
                     IVAGSNDKSVLYVSSENFTNQVINAIRNNAVGDVVNFYQQIDVLIIDDIQFFANKFKM
                     QEIFFNIFNQLHQSGRQIIMTSDRPPKDLEGIEDRLISRFKWGLTADLQTPDLETRIA
                     ILETKMARQGVDMPQNVSEFICYNIQSNIRELEGVLISLIAQSSLNRREIDIDLAKEV
                     IRSFVTQINKEITVEYIQKLVAEHFKVPVEKLQGETRKRSIVIARQLSMYLAKNMTDK
                     SLKAIGENFGGRDHSTVIYSCKTVQDLMETDPFFKDTVTELERKIKLSLNEKG"
     gene            23492..23581
                     /locus_tag="PK198_01275"
     tRNA            23492..23581
                     /locus_tag="PK198_01275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23526..23528,aa:Ser,seq:gct)
     assembly_gap    23594..23603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23628..23701
                     /locus_tag="PK198_01280"
     tRNA            23628..23701
                     /locus_tag="PK198_01280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23662..23664,aa:Pro,seq:tgg)
     gene            23883..>24521
                     /locus_tag="PK198_01285"
     CDS             23883..>24521
                     /locus_tag="PK198_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37393.1"
                     /translation="MHRFFNVLLCIAVCATFFSSCKKSAPPAWQQFMDCAGVDCTTQV
                     AAVKDLYLQDPTVLFEEFIKTDERGEDHFIGWLYLLRDSVLMNPNFASDVDRSEMQAA
                     ILDKTRPYENDAKYGSWAKSILMEIEPLSISLEMQPITGTYTYELPNDGGSGEIKIYP
                     SDDGTVRYAIAVVGSAPAFNQGMMEGNAPLKGNVVTITNTEFGGNCVIELTFA"
     assembly_gap    24522..24650
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24651..24946
                     /locus_tag="PK198_01290"
     CDS             <24651..24946
                     /locus_tag="PK198_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37394.1"
                     /translation="APAGLEGHWVSTSDVKSEVVIGNGKYVEIYDGKEVSSTPYRYFN
                     ACPEDCSPVAPTPCLQLMGQDILCYTVVKVSAKRLELSMIGGRGNTLAFVPKK"
     assembly_gap    25050..25198
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25199..26365
                     /locus_tag="PK198_01295"
     CDS             <25199..26365
                     /locus_tag="PK198_01295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF37395.1"
                     /translation="FIWGFLLLGALWAEAQEACTSTATDSICRHYFERYRQVGMAVGI
                     VHGDEAYQFAAGATDHSGRFPVTDSTLFHLASVTKLFTATAILQLAEAGKLRLDDALT
                     KHIPEFSMRDERYRDIQLQHLLTHSSGLMWDNKLKNSPDDAGSMPLFINQLKKKKLAF
                     QPGEKMSYSTYSNAGFDLLGMVVERVSGQSYDQYIPSRIWSQAGIAGATFYYETAEKA
                     LLALPQKLEGRSQKVRMLNRGLHYDSLAFSGPWPFPTITHPVYGEEYEHNASGNAIAS
                     AAELRLWMRHLLDIYRHDKKDGVLGRQRLADMWDTHQSATDSKAKIGWGWWISDEPFG
                     KMVFHVGNNPGFSSILLLYPDHDFGIVVLCNSNYAQLIVWNRFTEDLARVWLGR"
     gene            complement(26384..27022)
                     /locus_tag="PK198_01300"
     CDS             complement(26384..27022)
                     /locus_tag="PK198_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37396.1"
                     /translation="MKFIVGIGIAAAIFSIWIAVDLLYPVQTDFRRFDAPAVGRLETE
                     MWRSYYERNHFRLFRQLARLIRVQFKAPYWRSYLLAFHAARAAAVFQAGSNREEYGRA
                     WPHLNSYFSALRQMSDKPFQPETVSAYELEWWIVRREPDQFSPQDWERLLCATASEFY
                     GMPAAGFREYANLRVQAMLLRDSKGMGMSEQDWQSIRNLLENSWSHLHNELE"
     gene            complement(27027..27614)
                     /locus_tag="PK198_01305"
     CDS             complement(27027..27614)
                     /locus_tag="PK198_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF37397.1"
                     /translation="MNIVNSVPVSDKKNTYHFITRWVVNATSAEVYRTLEAVEDLARW
                     WPSVYLDVKQLEKGQPGGVGKLVALYTKGFLPYTLQWKFRVTQTQFPIGFSLEAIGDF
                     AGTGEWTFRDMNEEQCEVIYDWRIRAEKPLLKKLTWLLRPLFSANHQWAMRKGEESLK
                     LELRRRKAASDAERAAVPSPPGPVFPHNVTNNKVL"
     gene            27945..28487
                     /locus_tag="PK198_01310"
     CDS             27945..28487
                     /locus_tag="PK198_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37398.1"
                     /translation="MTKQIFAVLVSAVLLFLWQFLSWSMLGVHQDEYKYTPNQDKILE
                     ALNANLSENGDYFLPGVAPGTPMDQEQAFMENQAGKPWAKISYRKAFNTNMGMNLFRG
                     FVADLAAAFLLVWLLMKAEKRSFSSAMLSSLAVGFIGYLTIPYLDSIWYETKSIGFLI
                     DSVVQWGLVGVWLGWWLPKR"
     gene            complement(28550..29449)
                     /locus_tag="PK198_01315"
     CDS             complement(28550..29449)
                     /locus_tag="PK198_01315"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase family protein"
                     /protein_id="HRF37399.1"
                     /translation="MKTHFSFCAALFALLFTLPFTAFGGKYATSITAFWAGVDNGQFD
                     KAVTYLSPDLKVYLPLSPTPLNRDAYRQLGEGFRMGFPDISHKVLEVTEGKMTAAVRG
                     IFSGTNTGSLMGNPSTGNRVELPFLQYWTFDADGKAVRIEISFDLAAFNAQLMQGLPA
                     PINKATELALKMMANLRTHDLDGVIQYCAADARFNGWGPQPLDSKGYRQAMTEILESF
                     PDAAFTVSDVVSEGDKVVVRHQLEGTHTGAAFQGVPKSNRKIKVSATVTFYFNNGKAQ
                     ELWLNADMLGLLIQLGANPLTKN"
     gene            complement(29504..29938)
                     /locus_tag="PK198_01320"
     CDS             complement(29504..29938)
                     /locus_tag="PK198_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF37400.1"
                     /translation="MTAVFENQSAEVAKRNLAAAQLIYNLFLQGNIPAVLEMCSEDIV
                     WKHGANPHIVPFGGTYEGKAGVLRFFEAVSQSVQMTRFEPHNFAAEGNSVTNDVHVEL
                     MVIPTGKTLYLPESSVWTFGDDGRVVQYETCGDMSTLERAFL"
     gene            30129..>31290
                     /locus_tag="PK198_01325"
     CDS             30129..>31290
                     /locus_tag="PK198_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37401.1"
                     /translation="MRTGIAILWVLLSALGQPAMSQLDSLLQVARQSNSDTARVWAWM
                     EIGKLYLQSQPDTAELYLNQALHIAEKAGFERGVAKCRINRAFAFNNQGKYKKSVDDC
                     LVAVPLCRKLYMKKELVAAYNNMGNAWDYMGNRWQAIDAFSNALSELDAAGLPPHFPI
                     VVKNNITRQYANLGLHQKAYDYSLQSYEDAMLLGDSSLVASAVHHLASAALSLGRSEE
                     ALSHCRRVERLARQVNDPILLTFALNNIAVITWPETPAQAERDIREALSVAEQSGDLF
                     GKIGSLYVTARFAIWKGRPQEARKLVLEAMDEARKEGMDDEVATAYSLLSDIALATGN
                     MDEYRNMRRRFNFMSDTLMNNQLVVATQELETQYETAQKEQQIVALWQQKELQ"
     assembly_gap    31291..31331
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31332..32108
                     /locus_tag="PK198_01330"
     CDS             <31332..32108
                     /locus_tag="PK198_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF37402.1"
                     /translation="IGLAAAATAMLVLAALLWNVFQSRRKILGQEILIQQQKIKDLEQ
                     ERQLGFADAVMQGQEKERSRLARDLHDGLGGMLSGIKQAVFSLIGHSPKEAGTAAGLH
                     KIVQDIDQSIHELRHIARNLMPEALVRFGLQDALQDYCDHLQQPDKLHIHFQSFGMTG
                     RLARETEVVLFRIAQELLNNVVRHAGASEVLVQLLRDGHRLSLTVEDNGRGFYTPNLA
                     KVEGVGWFNIRSRVSYLSGNLDLKSAPNEGCSVHIEIPLP"
     gene            32105..32731
                     /locus_tag="PK198_01335"
     CDS             32105..32731
                     /locus_tag="PK198_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37403.1"
                     /translation="MIEVFIIDDHPMVVAGIQALLNHSEVVRICGAAHDAGTALEMLG
                     TSTAQVVLVDINLPDMNGIDLCVLMRKEFPKLKILGLSTFKERSYITRMMEAGASGYL
                     LKNASKEELEDAVVAANKGRAYLSMEVAATLAVAQSVPSAIPMLTAREKEVLHLIAEG
                     LTNQEIADKLFISILTVDSHRKNLLAKFGVKNTAAMIKTALELRLIAF"
     gene            32812..>33174
                     /locus_tag="PK198_01340"
     CDS             32812..>33174
                     /locus_tag="PK198_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRF37404.1"
                     /translation="MNFQQLEYIVALHAHRNFTVAAGHCFVTQSTLSSMIQRLEEELD
                     IVIFDRSKKPVEATPAGLEIIARAQKILAETAALRHYATELHQGLSGELRMAVIPTLA
                     PYLLPLFVGNFLRSYPDIH"
BASE COUNT         7672 a         8680 c         8443 g         7589 t
ORIGIN      
        1 ttcatgcgcg aggaggaaaa attggcccac gatgtgtatc aggcattgct gggacgctgg
       61 aatgtggagg tttttagcaa tatatcaaaa tctgaacagc ggcatacgga tgccattctc
      121 tgcctgctcc aaaagtataa tttgaccgat ccggtgggat ccaatggccc cggcgttttt
      181 caaaatgctg atttacaagc cctctacacg gttttggtan nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnngctacg cccgacatca acaatgccga tattctggcg gtatttggag aactcaccaa
      361 aggctcccgc aatcacctgc gcgccttcac ccggcagctc gtccaactcg gcgcaaccta
      421 tacgccgcaa tacatcagcg ctgaaactta tgcgcaaatc attgcttcgg agcacgaacg
      481 cggcggttct gcctgtgtgt ctaattgcaa cggaaaccaa cagggtaatg gatgcaacaa
      541 caaccgcaga ggacggcaag gcggcaacgg caattgcgac cacaatggct caggtaacgg
      601 cggcaatggc tgcaatggcg gaaatggcaa cggcgggaac ggaaccggcg gaaatggtaa
      661 tggtggtaac ggcaacggtg ggaacggaac cggtggaaat ggtaatggtg gtaatggcaa
      721 cggaggaaac ggcaacggcg gaaatggtaa tggtggtaat ggcaacggag gaaacggcaa
      781 cggaggcaac taatttttaa acaataactt ctcaaaatgc ccggagtgtg catcggattg
      841 cgcgctccgg gtttatcttt gatcgccgtc tgaacaaccg accatgctca tttcaaagaa
      901 aatatgcgat gaattggatg atctgcaaat cattcgcaag gcattggcgg aaaccgatta
      961 cttttcttgt ctgtacgaac gatacgaagc gcaactcctg cgatacgttc agcgggttgc
     1021 cgccctgtcg gaggaggaag cgcaggacgt gcttcaagag gcatttatca aaatctggcg
     1081 ccacctcaac gccttcgacc cccaacttcg actgtcttcc tggatgtatc gcatcgtaca
     1141 caatgaggcg gtgtctgctc tcagaaaaaa gacctcgttt ggcaaagatc aacaattagt
     1201 tatagacgaa tcacttcaat cccggttggc tgccgaatgg cccgatctgg gctacaacga
     1261 tgaaatcgaa gaccgggaac atgcgatccg tacggcattg tcggagcttg ccttgccaca
     1321 ccgcgaagtg ctggtgctgc gttttatgga agacatgagt tacgaagaaa tttccgatgt
     1381 gctcaaaatt cccgaaggca ccgtcgccac ccggctcaat cgggctaaaa aggcgcttgc
     1441 cgccgccttt gcatcctact catccaacac aaaaaggccg taaccatgaa aaactcggaa
     1501 cgacttatcc aagccatcaa agaacgccgg attgcaccgg tacccaaggc tttttttaca
     1561 ggacgggaac tgctgttgtg gttggtcttt gccttgtcgg ctttgctggg tgcggcggcg
     1621 ttttcggtca tacttttcag tattcagcaa actgattttt acctgctcca acacagcgga
     1681 cactcccggc tggaactgtt gttgagttgg ttgccctttg tttggatggc gtttctgttt
     1741 gtgtttctgg ggctttccat gttcagcttt cgcaatagcc ggcgaggtta caagtggcgg
     1801 ctgactcatt tggcagcgta cagttttgga ttcagcgttt tgttgggcac cgcgtttttt
     1861 ttggcaggcg gcgcggcacg tcttgaacgg gcattcgatg ttcgggtgag catttaccaa
     1921 agtatggagg aaaaaaagat aaaagtatgg tctatgcccg aacagggcta cctttccgga
     1981 gtcatagaaa ccgttgatgg agaggaaatg accatccgcg attttgacgg aaaaatctgg
     2041 gccattgaca tttccaacgc ttttgtagcg cccgtgctgg ctttggacgc aggcgagcaa
     2101 gtaaaactga ccggccggat gacctctgaa ggccggtttg aagccgatgg cgttcgcccc
     2161 tggggtgggc cgggatttcg gcatcagaaa aaatagccgc tcgattttca aaaagtccta
     2221 cctttgccat tatgaatcgc ttttgcggca tattattatt ttccgcgtac gcctttgccg
     2281 tgttggttcc gcctgctttc aaacaagaac tgggcaagtt ggaccacctc ctgaatcact
     2341 atcaggagca ccgcgcggaa acgcccgata tgtcgctcat cgcctttcta aaaatgcact
     2401 acggagagca atttgccacc cacagcgccg accacagcca caacaacctc cccggcaaaa
     2461 cctctcagga tcatacccat gccgtagctt gcggttgcag cgtttttgag ctgatctgcc
     2521 atttcgattg gagcctgcgc cgtccagcac cccaacaaca tacccttttt cccaccgaaa
     2581 taaaccttct ctccaccctg cacgcctccg gcatctggca gccccccaaa cacgtctgac
     2641 ctcaccactc accccactca cacactcaca cactcacaca ctcatcactg attcaacatg
     2701 ttagacgcca tcattcggct gtctgtcaac aataagctca ttgtgggcct cttcactgcc
     2761 gcgctcatct ttgccggcgt atatgccttc gttcagctac cgatagacgc cattcccgat
     2821 attaccaaca accaggtgca ggtactcact gtgagtccta ccctggccac tcaggaggtg
     2881 gaacaattcg tcaccgcccc cattgaacac gccgtgcgct ccattcccgg cgtggtcgaa
     2941 ctgcgctcca tctcgcgttt cggcctcagc gttatcaccg tcgttttcga ggagcgcatc
     3001 aatccctatc tggcccggca gctcatttcc gaaaagctca aggtagccga agaaatgatc
     3061 ccgcccggct tcggcacgcc cgaaatggcc cccatctcta ccggcttggg cgaaatcctg
     3121 cactaccgcg tagagccgct gccgggctac gaaaaccgct acaccgccac cgatctgcgc
     3181 accattcagg actggatcgt aaaaaggcag ctctccggca ttcccggcgt ggtcgaaatc
     3241 aacagcttcg gcggcttcct caaacaatac gaggtggccc tctcgcccga acgcctgcgc
     3301 agcttcggca tcggcatcca ggaggtgttt caggccctgg aaaacgccaa cgaaaacacc
     3361 ggcggcgctt acatcgagca taccaacaac gcctatttca ttcgcagcga gggacttgtg
     3421 caatctttgg acgacatccg ccaaatcgtg gtgcgcgaca atgccggcgc tcccatcaaa
     3481 atcggcgaca tcggcgaggc ccgcttcggc catgccgtcc gctacggcgc cgtcagtcac
     3541 aacgacgagg gcgaaattgt gctcggcatc gtcatgatgc tcaaaggcga aaacgcagcc
     3601 cgcgtcatcc gccgggtcaa agagcgcctc gaagtagtca aaaaatcgct gcccgaaggc
     3661 gtcaccgtca cgcccttcct cgaccgcgaa aacttggtca atcgcaccat cgccaccgtg
     3721 cgcaacaacc tcaccgaggg cgccctcatc gtcatcttcg tgctggtgct gctgctgggc
     3781 aactggcgcg ccggcctcat catcgcctcc gtcatcccgc tgtccatgct ctttgcgctg
     3841 tggatgatgc acctcttcgg cgtcagcgcc aacctcatgt cactcggcgc catcgacttc
     3901 ggcctcatcg tggacggcgc ggtcatcatc gtggaggcta cgctgcactt cctggctgtc
     3961 agggttttac aaaaacaggg agagggcgcc acccgcttca cccgcgccga aatggacgaa
     4021 aacgtcagca cggccgccag ccgcgtcatg cgttcttcag tcttcggcat catcatcatt
     4081 ctcatcgtgt atctgcccat cctttcgctg tcgggcgtcg agggcaagat gttcagaccc
     4141 atggcccaaa cggtgtcctt cgcgctcatc ggcgctttga tcctgtcgct cacctacgtt
     4201 cccgccgtgt cggctttggt gctcagtcgc cgaatcaaaa cgacgcatac cttctctgac
     4261 cgcctcatgg agtggttgaa aaaactgtat gcaccctcgc tcgaactcgc cctgcgcttt
     4321 cccaagttca tcatcgtttc tacgctgctc gtcttctcgc tcagcgtcgg attgttcctg
     4381 cgcatgggcg gcgagtttat ccccacctta gatgaaggcg acatcatgat gcacggtttc
     4441 ctgcggcccg gcacctcgct gacgcagact ttggaaagcc atcgcttggc gcaaaaaatc
     4501 atcctcgaca acttccccga tgaagtcgag caggtcattt ccaaaatcgg ttccgccgaa
     4561 attcccaccg accccatggc catcgaaacc gccgacaaca tcatcctgct ccgcgacaaa
     4621 tcgctctgga aacgcgccgc taccaaggcc gaattggtag aactcatcga ggcaaaagtc
     4681 cacgagattc ccggcatggc cttcgagttc acgcagccca tcaaaatgcg cttcgacgaa
     4741 atgatgactg gcgtgcgctc cgatattgcc gtcaaaatct tcggtgaaaa cctcgactcc
     4801 ctcgcccgcg caggccaccg cgtcgagcgc ctcctctccg ccatcgaagg cgtggaagac
     4861 gtcaaaaccg agcaaataga gggcctcccg caaatcgtcg tcaagttcaa ttacggccag
     4921 atcgcccgcc acggcctcca catccgcgat gtcaacgaga ccatccgcgc cgcctttgcc
     4981 ggggccaccg ccggcaccgt ttttgaaaac gaacgccgtt tcgatctcgt cgtacgcctc
     5041 aataccctcc accgcagccg catcgaagac gtgctccaac tgcccctcag cacgccctcc
     5101 ggcgcgctca tcccgctctc cgaagtggcc gaggtgcgct tcctgcccgg cgccgcacag
     5161 atcagccgcg acgatgccca acgccgcatc gtcatcagtg ccaacgtgcg tggccnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctccatcgc
     5341 cgtgccggtc gctttggccc tcattttcat gctgttgtat ttcgcgttca actccctcaa
     5401 agaaggcttg ctcatcttca ccgccatccc catggccgcc gtagggggcg tattggccct
     5461 gctgttgcgc ggcatgccct tttctatttc ggcaggcgtg ggcttcatcg ccctgttcgg
     5521 catcgccgtg ctcaacggca tcgtcatgat cgcatatttc aatccactcg aaannnnnnn
     5581 nnnnnnnnnn nnnnngcttg atgcgccagt aggggcggga gaagttcacc gccttggcca
     5641 tgtcctccag cgagggcatg ggattgtggc gctcgggctc gctctcgcac ggcgccggcg
     5701 ccgaagtgca acgccctttg gccaccgtcg tcatcggagg cttgctcact tccacgctgc
     5761 tcacattagt ggtactgccc gtcctgtacg ccatgttttt caaatcaaac caagaacaat
     5821 gaactgcata aattcattca ttatcaatat tttaagacag gtttttgtgg cggctaccca
     5881 caccggctcc acccccggcc cctccaaagg aggagagaaa gcaggcggtg ggtaccgggt
     5941 cgttcgctct annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccgcc gatctctttc acaacatcgc
     6181 cgccgacccc gactacggca tgttcggcac cacggctttc ggattcaatc aggctttccc
     6241 ggcccgcaag atgacgcagg cgcgccgcct ttattaccag cgccgggaag ccgccgctgc
     6301 cgccgggttc atgctcacgc gcaggcaggt agcccgctcc gtcagcgaac tctatcagca
     6361 catcggcttc atacaaagcg aagcgggact ctatcggcgt ttagacagcc tctaccagtc
     6421 ggtggcggcc attgctcagt tgcgctacac tgccggcgag gtttccaaag cagagcagct
     6481 cgccctcgaa gacaaagccg cccagatgcg cctggccctc caaaccaccg gccacgaaat
     6541 cgaattcgac cgcgtcgtat tggggcaggt gctgggcatt ccggggcagg tatttcccgt
     6601 gtcggggccg tttcagccca tgagcttctc ccttgccgat acggccttag tagaaaactc
     6661 cgcccgttct gtattgtacc gtacgtccat agccatctcc gaagccgaac aagaagtcgt
     6721 agccgcccag cgtgcgcctg tattctccgg cggcctcatg gtgcaatacc tgcccaacgg
     6781 acgagtgctg cccggctggc aattggggct gcaaattcca ttgataacca aaagtttgcg
     6841 tgccgatcaa gcggctgccg ctctcggtgt tcagtctgcc gccgccgact accgcgccga
     6901 cttgctggag cagcgcaccc aattggcgca cctcctgcac gagcaggaaa agtaccagat
     6961 tcgattggag tattacaccg ccaaaggcaa ggcattggcc gatgagttat tgcgcatggc
     7021 tgtagccaac taccgcgccg gcgaaatcgg ctttgttgaa ctcacacaat tcgccgaaca
     7081 ggccgccgcc attgagttgg actatttaga aaacctgctc ggcctgaaca tggccatcat
     7141 ccaattgcgc acgctgacgg ggcagtgaat gactacttag cattttattc aaagccacaa
     7201 ccgcgccgtg gcgcggcatt agccgttagc ttatttttcg ataaggagga agattttcct
     7261 catggggaaa tttctaaatg cacttttgaa cacgaattct tcttcttttg gaaaaccaag
     7321 ctaatgccac gcaaggcgtg gtaatagcta acagcctatg tgaaatgcca gatgaatact
     7381 aaaattaaaa aattctcacc atgaaaaaat acatccttct ttcgggtctt gccctcatcg
     7441 cggcggcctg cggcaccaaa aatgccgaac ccgtttccga agcgcccgcc gccgaagaaa
     7501 tccacgagcg catcagcctc agcgccgagc aattccggca ggccggcatc caaaccggca
     7561 ccttcagcta ccgcaccatg caggagtact tcccggcggc ggcagaactg tacctcggcg
     7621 cagaacatac ggcatccgtc agcaccatca ccgagggcat cgtttcggaa ttgcgggtca
     7681 cgctcaatca gacgctccgc aaaggcgatg tcgtggcggt gctgcacaag cccggtctgc
     7741 tcgattggca ggaggaatat ttagagcgca aagaccgcat cgcatttttg aaaatggagt
     7801 tggagcgcta caaaaccctc gctgccgaca atgccaccgc cacaaaaaat ctgcaaaaag
     7861 ccgaggccga cctccgcgag gcgcaaacct cccaaaccct gctggctgcc aaactgcgtc
     7921 aataccagat tgatcctgaa ttggttacga catccaatct caaaacgcaa atcatgttgc
     7981 gcgcgcccat ttcgggtacc gttacgaaaa tccattcggg actgggcgct tctttggctc
     8041 ccggcgctgc gctggtggac atggccgatt attcgcgcgt tcagccggtg gtgtatattt
     8101 ttgaaaaaga cattcaccgc gtgaaagtag gcgcacgggt gctgttgttt tttgccgccg
     8161 accccggccg taccttcccg gctgttatta ccaacatgga cggcgccgtg gaccgcgagc
     8221 ggcgggcgct gcgcgctttt gcccgtttcg agcagtcgcc tgataagctc gctgcggggg
     8281 catacatgga cgcgaaaatt gcgccctccg caggcagcga aacccaggca ttgccggaag
     8341 ccgctgtggt gcgcgagagc gatgggcagt atatttttat tttggaactc gaacgcgcag
     8401 acggggtcat attccataaa gtggcggtac aaacaggagc cgtggaaggt ggctttgtgg
     8461 ccgtgacgcc gctggagcag ttgccaaagg atgcaaaaat cgtgttgcag ggcgcgtatt
     8521 atgtgtcggc tcagggcgcg gggatagagg tggaggagtg aggcaggggt gagtgagtca
     8581 ggggtgagtg ggtgaggggt gagtgggtga gtgggtgagg agttgtatct ttgcctcata
     8641 acttatgcca ttgccatgaa gaaatacagc ttgctctttc tgccgtgcct gcttttgtcc
     8701 gtctgcctga cggcgcagcc ctgtcctgat aaaccatata tcaaagcgcc tgaattgtcg
     8761 gatgcagtcc gcaagacttt tgaggctcaa ttggctcagg ctcagacgac ttacgaggct
     8821 cgccccgatg atgccgatgc gctgatatgg atggggcgtc gcaccgcata tctgggcagg
     8881 tacatggagg ccattgagtt gtttggaaag ggcgtggaaa agcatcccgc tgatgcccgt
     8941 ttcttgcggc accggggtca tcgctacctc acggtgcgct gttttgatgc ggccattgag
     9001 gattttcagg ctgccgcccg gctcacccgc cgccttcccg acgaggtcga acccgacggt
     9061 ttgcccaatg cccgcaacat tcccaccagt actttgcaat ccaatatttg gtaccatctg
     9121 gcgctggcgc attacctcaa aggcgatttt aaaaaagccc tgcccgcctg gaaacgttgt
     9181 gtggagcttt cccaaaatcc cgacgggctg gtttcttcta cgcattggta ctacatgacc
     9241 gcccgacgat tgggtctgca aaggaaggcg gaaaaggcat tgcagaaagt cggaaaggat
     9301 ttggatatta ttgaaaatca ggactattat accttgattc agttgtatca gggccgaata
     9361 ggggaggggg atgttcaaaa aatgctgagg actaccgaca acgcgctgag caatgcttca
     9421 ttgggctacg gctatggcaa ttggctgtgg tacaatgggc ggcagcagga agcccggcag
     9481 gtttttgctc aaatcacgca gggcagctcc tgggcggctt tcgggttcat tgcagcggag
     9541 gcagagttga gccggaaata gaaaaaggcg ctccgtatcc gaagcgccca cacaaactat
     9601 aaacaaacaa aaaacattag cgaaagaata tcgggataac aaaggagtaa aggttttgtt
     9661 ccaaaaaaaa tggccttata tcatctttcc cgcaaacgca aattcagcca attccttgcg
     9721 tttgcgcggc gtcagttcgc gggtacggaa gggttcctcc aacttttcag gttcgtccaa
     9781 atagccgatg gagatgaaac aaacggcctc catgtcgtcg ggaatttgga aagtttcaat
     9841 ggttttttgc atatcgaagc cgcccatctg atggccgaaa atgtccatag cggcggcttg
     9901 cagcagcagg gtggtattgg ccgccccgac gtcgtgcagg gcatggcgat tgtgtttgcc
     9961 gttggaggcg aagttcttgc gggcaaggct cagcagcaac acgggagcgc cgtcggccca
    10021 gcggtttccg ggcaggaggc agtcatacat ttgctgaaaa acaggctcgc ctttgtgcgc
    10081 gtacagatat acccagggct gctcgttcat gctgctcgat gcccaggagg ctgcctctaa
    10141 gagggtcatc agcgtatcgt gttcgatggg gcggtcggcg aaggagcgcg cactccagcg
    10201 ctgacgaatg aactcatgca caggatattg cgtggccgct tctttggcgt ttatgctttc
    10261 cggttggaga atggtatcca tattattgat gtcttgcttt cttgaaagct gttgaaacaa
    10321 accgatggcg ggagtgttca atgagcgatt tgcattttgg cgaatatgag tgtatctttg
    10381 cacccgaaat gaaacgcaaa ctgtccatat cactgctttt tgtcgcgctc ctcatggcgc
    10441 tgtcgccctg cgtggaggtc ttcagttttg cgttgcaacg ccatttttcc cacagcgaaa
    10501 tgtcttgctg cagccgtccc tccacggtgg aagaaaaagc aggcaaatcc ggttgcaaaa
    10561 aaacggactt ggctgaaacc ggcgcttcct gtgggcattc cggtgaagaa caacacggac
    10621 agtgcggctc ccagtgccat tgctcctgct gcggacatgc cgctacgttt ttcattgcgt
    10681 atctgtttga ttttcagtat atagagatag cgtatcaaca accctgttat agctccggtt
    10741 atcatttcga ttacacagaa accatctggc agccgccccg catagtggga tgattttggg
    10801 gcattgtagt tgctcagaac tattcagttt caagctacat gcctcaagtc ctgtatcccc
    10861 tagctcgtag ctcgtagctc gcggctcgta gcttttggac gggactgaat agctattttt
    10921 catccatata ttcctccttc cggcgaactg ttcggcgggc tgtggatatg taaaatcatc
    10981 caaccatgcg aatcattata ttcaggctca gcgccatatt actgctgttt acctcgctgt
    11041 cggcagcagc gcaaaatcaa gatcataaca ctttgaaaat ctgggtaaac ggtgcctgcg
    11101 gtatgtgcga agaccgcatt cagaaaaccg cactgcgcac gcccggcgtt cacacggccg
    11161 tttgggacga gcacaccaaa atgctgaccg tcaccaccgg ggccaaattc aaagaggacc
    11221 aattgcacca gaacattgca caagtcggcc acgataccga aaaagtaaaa gcgcccgatg
    11281 atgtgtatga aaacctgcac gcctgctgcc actacgaccg cagcgatgtg ccgccttccc
    11341 aaaaatcgaa gacgatcaga ggcaaggttt tggaggagct gaaaaaagga gaagaatcgc
    11401 cgctcatcgg ggcggtagtg cattggctcg gcacctcgca gggcagtacc accgatgccg
    11461 acggccattt tgaattagag ccgcatccgg gcagcaatcg cttagtagtc agttatatcg
    11521 gctaccggcc cgacactgtt gaggtgaagg acggcttcga cttggacatt gtgctgtcca
    11581 atacgcatac gctcaaagag gtggaggttt tttacagaaa aaaagccacc gaggtttcct
    11641 ttttctcgcc cattcaggtg caggtcatca gtgaaaaaga gttgctcaaa gcggcctgct
    11701 gcaacctctc ggagagcttt gaaaccacgc cttccattga tgtcaatttt accgacgctg
    11761 taaccggcac ccgccagatt caactgctcg gattggccgg tcctaacatc cagatcaccc
    11821 gcgagagcat gcccgatgtg cgcgggttgt cggccctgta cggcatgaat tacaccgccg
    11881 gctcctgggt cgagggcatc caactgagca tgggcgcggg ttcggttgtc aatgggtttg
    11941 aagccatcgc cggccagatc aatgtggaat tgcgcaagcc cgaaacctcc gacctgatgt
    12001 atctcaacct ctatgccgct acgatgggcc gctacgaggg caacgccaat tttagctaca
    12061 aaatcaatga gcaatggagc ggcggcgtcc tcttgcacgg caaaagccag cagcgcgagg
    12121 tggaccacaa ccacgacgga tttttggaca accccctcag cgatgcgctc atcgccatca
    12181 aacgctgggg ctttcagggc aaaaaaggct gggaggcgca gtttggtctc aaaggcgcac
    12241 tgctcaatga caagagtggc gccacgggct tccatgaaga cgtggagctg cataccggcc
    12301 actggtgggg cgcatcggtg gccaccaaac gcgccgaagt ctgggccaaa atcggcaggg
    12361 tgctaccgaa taaaccccac actacctacg gcctgcaact atcggccacc cgccatgagc
    12421 agcgcgcgta cttcggcctg cgcagtctga acgccgacca gaaatcggct tacgccaact
    12481 tcattttcca gagtatcatc ggcgataccc gccacaccta ccgcaccggc gcgagttttc
    12541 agtgggacaa ttttgaggaa aacgccaaca accgctggta tgacagaaat gagcagattc
    12601 ccggcgcttt ttttgaatac acatacaaac ctgacgataa gttgacggcc gtggcaggca
    12661 ttcgcgccga ctaccacaac aactacgggc tgttttttac gccgcgcctg cacgtttcct
    12721 acacgccccg cgatctgacg gtctttcgca tctcggcagc cagagggcag cgcaccgcct
    12781 ccgtctgggc tgaaaacatc ggtatctggg cctccaatcg caacatcgtt ttgcatggcg
    12841 atgccgaagg gcttccctat ggcctgcgcc cggaaatagc ctggaatttc ggtctcaact
    12901 tcacgcaagg ctttgaaata aaccaccgcg cggctacttt cacggccggc gtgtatcaca
    12961 ccaattttgt gaatcaggtg gtggttgatt tcgacaaagg tccgcaggaa ctgcacatcg
    13021 gcaacttgga cgggcgctcg ttttccaaca gcgctatggc tcagttggat gtggaattgc
    13081 tggcgggctg ggacctgcga ttggcctacc gtttcaacga cgcccgcacc acctttgcgc
    13141 ccggcttgct ggaacgcccg ctcttatctc ggcaccgggc ttttctcaat actgcctacg
    13201 aaaccaaaag cggctggcat ttcgacttca cgctcaactg gcagggcgct cggcgtattc
    13261 cgtccacggc ctccaatccg gcnnnnnnnn nnnnnnnnnn nnattggagc gctcgccgga
    13321 ttacttcctg gccaatgcgc agatcagcaa gcgctggggc gataagtacg atttgtacat
    13381 cggggcggaa aacatttttg catacacaca gcatcatccc atcatcggcg ccaatgatcc
    13441 cttcgggccg tatttcgaca gcacgctggt atgggggcct atttttggtc gcaatatcta
    13501 tgtgggaggc agatggcgga tagcgaggag gtgagtcaag ttttgaaggg gtgactgcgg
    13561 gcagtgatct cggacggggc gccccttcat tgctcataat tgtaagcagt tgagttgagt
    13621 acactgtttt ttcatgtcaa acattcgcta tctttgccct tatgaaaaga tacttcaaca
    13681 ccaccgggtt ttgtaatcct gaaaagcact acatggtcaa tccctttcgg gatatatacg
    13741 atgacatttt gcggcttgtc gaagctgagc agtattttct cattcatgcg ccccggcaga
    13801 gtggcaagac taccttcctg cattcgttgg cgcaccgttt gaatcaggag ggaaagtata
    13861 tatcagtagt gggatcgctg gaggcagcag ggtactccag cataactgta gcagatgcca
    13921 attgggtgat ggtcaagtct ctgtatcaaa acgcaaaact ttttctgact gaagactatg
    13981 tgcctcggaa tccggatcat tataccaaag actcttcact ttttcagacg tacctgatgg
    14041 actggtgcga ggagctgcct aagccggtcg tattgctgtt ggatgaagtg gatgctctgt
    14101 acgacgacgt gctgatatcg gtactgcggc agttacgcga tggtttccag cggcgtccga
    14161 agttgtttcc acagtcaata gccttggtgg gcctgcgcga catccgcgag tacaaaatgc
    14221 gcgcccgtgc cgacaatccc tccattggcg ctggttcgcc cttcaatgtc aaagccgagt
    14281 cgtttttctt acccgtcttc agcctcgaag aagtgcgcgg ccttttgcaa caacacacag
    14341 acgacaccgg gcaggttttt acagaggagg tactccaaaa actatacgaa tactccggcg
    14401 ggcaaccctg gcttaccaac gcattggcca accaaattgt ggtcaaaata ctgaaaggag
    14461 actactcccg tgccatcacg cccgatatgg tggaagacgc caaagagcgc ctcatcgcgc
    14521 agcggcaaac gcatttggac agtctggccg ataaaataca ggaagaacgt gtgcgacgca
    14581 tcgtcaccaa catcatttcc ggtggagata tacaatttga cggcgccgac gacaatctcc
    14641 gctattgccg cgatctgggc atcattacag ccaagagtcc ggtgcaattt gccaatccca
    14701 tttaccggga aatcatcacc cgcatcatga gtgccggttt tcaggagagc atcaatcagg
    14761 acatcgccca aacctcgtgg tacctgcgac cggatggctc gttggacatg gacaagctgt
    14821 tggaggcttt tgtggatttt taccgctgga attccgaatc ctggctcgaa cgctttcagt
    14881 acaaagaggc cgggcatcaa ctgctgctca tggctttttt gcaacgcatc atcaacggag
    14941 gcgggcgcat agagcgagaa atggccatcg gcaacgggcg tactgactta gctgtttttt
    15001 ggaaagatca ggtcattccc atagaaatca aaatgcacca caatgcccgc tcccagccgg
    15061 atggcctgcg ccaattagcc cgctacatgg acaagttggg ccaaaagcaa ggctacttta
    15121 ttctttttga aaaaaagtcc tccgaagagt tgtcttggga agaccgcatc cgcagggaaa
    15181 tacacgaggt ggatggcaag gaggtgattt tgctgggaat gtaggcagga tggataaata
    15241 ccgcccccta gtccccctcc tccaattcaa aaatctcctc cacgggtttc tgaaaaaggg
    15301 ccgcgatctt cagcgccagc actgtagaag gcacatagcg gttgttctcg atggagtgaa
    15361 tcgtctggcg gctcacgccg atgcgctcgg ccagttcctg ttgttgcagg ttgaggcggg
    15421 cgcgttcttc ttttactcgg tttttcataa gatagaattt agctttccgg cgccggactc
    15481 ccggatgtac aatacataat aaaaccgcag caggaacacc actaagggtg tgaagaggtt
    15541 gaaggtcatc agccagaaaa agctgccgcc atacaatacc caattgccga tgaggacgat
    15601 gatgaaattc accagaatgc tccattgaaa cgcctccaag cgaatgaggc gcacgcgctc
    15661 gtcttcatcg cgctcgcggg tgaaggccag aatgagcagg ccgacaatgg tgagtgtcag
    15721 ggccacttcg ttggtgaaat cctcttctgc tgtgtctaaa aaagagttct tttcccgaac
    15781 tataaacttc agccaactaa aactgaactc gcccagttcg gcggcaaagc ctaaagccaa
    15841 acccagaatg ctcagaatgg cgccgggcca tttccatgtg acaggaagca aacgcattgt
    15901 tttcatgttg taaagatttt tttacaaagg taaagtattt tttacaaaag tcaagtttat
    15961 tttacatttt taactaaaaa aagaccattc agagtcttca gctctgaaac gatgccgtca
    16021 ctttgtgtta gtcggtactt ggccggggaa tgctcgtgag gacataggcc gctgtaatga
    16081 ttgtcacgag agttgttgcg acaaaggccg ttgcggctcc aaaccggaac caaaggaacc
    16141 ctgtcaatgt gcttgccatc atggcgcaga tactttgcag ccccgaaaat gtgcctatgg
    16201 ccgttgcggt gtccttttta tcagaaatat tggatatcca ggctttggaa atgccttcag
    16261 tggccgctgc gtaaacccca tatagaaaaa acaggccgag aaaaccgtat atattggtgt
    16321 tgaacgccat gccgaaatat acggctgcga ataccagcag gccgctcagg aacacagttt
    16381 tcaggccgat tttgtcggca agagcgccca atgggaaggc acacagcgca taaatgagat
    16441 tgtaaaaaac atatacgccg atcacatggg tgtcgtcgag gcctgactct ttcgctttga
    16501 gcagtaaaaa cacgtcggaa ctgttgaaca acgcgaacgc cagaagtccg acgaccagct
    16561 ttttgtacgc ggccgggctt tctttccaat accttagaaa ggaaaaaaag gaaacgggcg
    16621 ccctgctgcc ggcggcggtt gtgggcttgt ctttcagcaa cagcgaggca aggatggcaa
    16681 aaaggcccgg caggaaagcg atgtaaaaaa gcgtcttgta gtcgttgggg cgatggtgca
    16741 gataaagcag ggccgtaaga gggccgagta ccgcgcccag cgtatccata gagcgatgaa
    16801 aaccgaagac tttgcctttg gtttgcgggg tagcttcatc cgaaagaatg gcgtctcttg
    16861 cccccgttcg aatgcctttg ccgaggcgat caatggttcg ggcaaaaaaa atccagaggg
    16921 ggtagatgaa aaaggccatc atgggtttgg atagcgcgct gagggcgtaa ccgacttgaa
    16981 caaacggagc gcgcctgccc atagtgtcgg aatatttgcc aaagtagccc ttgctgagtc
    17041 cggcggttgc ttccgcgatg ccttcgagga tgcctatgag caccaccgag aaccctatgg
    17101 ttttcagata aatgggcata atggggtaga gcatctcgcc ggctgtgtcc gtcagcagac
    17161 tgataagcga taaaatccag acggatcgcg taatgtgttt catcggtaat gacctacttc
    17221 tcctccggcg tttcgatcaa agggcggtca ttttcgaaat cgtacagcgt aatgcgacgc
    17281 aaatcgtaat acgccagcca gaaattgcgc aatgccgaca catagctgcg ccgcgcctcg
    17341 gcctcctcgc gcagggctaa gttgaggtcg gtgatgagaa tttttccgat catgtagcga
    17401 ttgcggctca tctctaagcg ccgtttcgat acgtcgtgcg cggccatcgc caactgcacc
    17461 tggtttctga ccaaatcgaa ctgatgcacc ctgatgagaa tttcgcgctc aaaattgatg
    17521 cgttcctgcg ccacctgcat ttctaccaac tcctggttgg actgagcgat ttccagattg
    17581 gcccgcgacc gcccccagtc ggctatgggc aaagtaatgc ccagcaccac ttgttcctga
    17641 tcgagcggcc gcgtgtaggc cgtttcaata tcggggccgg tttgggagag gccgaaggag
    17701 ccgaacagat tcatttgcaa accccgttcg gctctggctt gggcaatggt catttgcgcc
    17761 tcggcgttgc ggcgctcgaa ggccagcacg tcgctgcggt tcatgcgggc ctgtaggagg
    17821 gctttgtggg cgtccacccg gaaatcgggg atgccggccg gcgtgacgag ttggaagacc
    17881 acggcagtgc ggatgcccag aaaatcgcgg aggcgctcgg tagcagtttg catggtcagc
    17941 gtttgttggg caatggcggc ttcggcattc atagcgctga gttcaatttg cagcaactca
    18001 tccaaggcaa tgcgccccac ttcaaaacgg cctttggata tggacagcag cgtatcggca
    18061 tcggctttgt ctttgcgggc agcctcaaga ctatattgag ccatcagtac atcgaagaag
    18121 agttcggtgg tgcgcagggc ggtttcggcc atgttttccg agtagcgttt ccgcgattct
    18181 tcgtagcgca gcggctcaat ttttttgccc catttcagtt gattgaatcc gaaaatgggc
    18241 tgctcaaaac caatagaaac aggtgtggac aggtaagaaa tgaaaccggg cttttcatct
    18301 gtgccgaaaa tatctatgcg ttccagattg gtgcgcatga acaccctccc gccggtagcg
    18361 gccacacctt gttgcagcga aatgcccgcg ccgttggtca tcaacgagcg actgatgaag
    18421 gcatcctgac cgttgggcag cgtgatgggc tgtatgctcc ggttcaggtt gggcagcgta
    18481 gcgttgaagg tgatggccgg cctgtaattg gccaaaaaag actggtatcg ccaataatta
    18541 ttggaataaa tggtgcgcgc tatggccaca tcaggcgcat cgctgtggga tatttccacc
    18601 gcctgagcca ggctcagcgg aatggtgtcc atgctttgcg aggccagccg gctgcacata
    18661 accagcatca acaaaatcag gtatgatcgc atcctctcgt gtttgttttc aaaaagtaaa
    18721 tagttgctat atatctgaaa aacagtattt tactactgct cacttataac cgccaccgca
    18781 aaccgcccac ccctcgccac aggcgcggct actgatgact gtctcactcc cgcctcagcg
    18841 ccgtaatcgg atcctgcaat gctgctcttc tggccggaaa aatgccaaaa atcaaaccta
    18901 ccgccgccgc cactgcaaac gaaaccagca ccgaccaagg ggcaatgata gtaggaatat
    18961 ctgccgaggc ggcaataatg cgggccgcca gaacgccgag cagcactcca atcatacctc
    19021 caaacaggct gatcatcacg gcctccagca aaaactgctg cacaatgtcg ctctgccggg
    19081 cgcctaatgc gcgccgcacg cctatttcct gtatgcgctc cagcaccgaa gccagcataa
    19141 tgttcataat accaataccc cccaccagca acgaaatggc ggcaatggcg gccaacacca
    19201 tattaaaggt gtcctgcgtt ttttgctcct gttgcaacag cagttccggc acctccacct
    19261 cataatccac cacatcgcgg tggcgacgct tgagcatgcg ggccatcaga tcggcggcgg
    19321 caggcagcag cttggatgat tccatacgaa tgacggcccg atctatttga tggtaattgg
    19381 ccgatttgcc ctcgtcgttg cctcgaccca gatcgctttt gctgatgacg gcccgatttt
    19441 caaaacgcag caaggctgtt tgaatcggaa taaaaacatc cgtattgtag tccttaatac
    19501 ccagattgtc aagcgtttcc ttaccggcat ttcgcttttg caacacacca atgaccgtaa
    19561 accaggtgtt gccgcatttg aattttttcc ccatcggatc agccccggcg aaaaagcggc
    19621 tctgtacgtc tttgccgatg atgcacacgg gcctgcccgc ttcgaagtgt tcggcatgga
    19681 acagctttcc tgctcccaat tccaaacgat tgagatagaa aaatgtgttt tccaccccga
    19741 tacaacgtcc tttttcctgc ttgccgccat agacgaaggt ggtgggcaac acgatttcag
    19801 gactcacaga ttcaacgccc ggcacgttct cccggatggc ctgtatatcg gcaaggctta
    19861 atcccggcga ccaggggcgt ttgtctttgt tgttcccgtt tgcgttgctg ccgcccgatg
    19921 aagcttcttt ttcagggtcg ggcacaatgg cctccagtac aatgttgttg gcgccgatga
    19981 gccgcatctg atccaaaata gcccgtttgg cgccgctgcc gatggagagc atggcgataa
    20041 ccgcggccac tccgaacact atgcctaaag cagtgagaag cgacctcagt ttgttggcgt
    20101 tgatgccccg caatgccagt ccgaaattaa aaagccatcg ttccatgcgc tctattccat
    20161 gataatgatg aaatcgcctc cgcttccgcc gtcgctggtg ggaacgtcta ttccctccac
    20221 cgtttttcgt ttctcggcgg cttccttccg ccgtcgttcg gcagcctttt cctgctcttt
    20281 tttgatggcg gctttgatgt cgggtgcgat gggaatcatg ggcagttcgg atgtgtcagg
    20341 tggaggggcc agccatacca cgtcgttttc ttccaggcca tgctctataa tgatctggtt
    20401 gtcgttgctc agcccggtga tgacctcctg gcgcaccaat ttgccgtctt tttctttata
    20461 aacaaaggtg acgctgtccg attgcagcgc ctctaagggg atgaagagct tgtcgggata
    20521 agagccggtg aggatttcat tgccggtggt catggccggg cgcaggatgg aatcgctttc
    20581 gctgacctgc acaatgacct caaacacttt ggcatcgtag ccttttattt gctctccgat
    20641 attggccacc gaaatgactt tgccggtgta gctgcggtcg gggaaggcgt ccactttaat
    20701 ggatacatcc tggccgggtt gcactttgct cacatccacc tcgttgacgt aggtttttga
    20761 aatcatatcg gtgaggtcgg gcagttcggc tactacgggg tcccaactgg aaatgcgcga
    20821 tccgggttcg cgtttgccgt tccaggtgcg ggcgtaaata atcatgcccg atttgggagc
    20881 atgaatggaa aactcgccgg atagcttggt caaaatgtct aattgttgtt ggttttgcct
    20941 gagtaaagca ttgatttcct gtattttagc aacagactgc tgacgtttta aatcgtattt
    21001 tttttccaat tgcctaaaat cgcgctctac ccgttccaga tcaaggcggg ccgtttggat
    21061 gaccgattga ggttcgtacc ggctttggtc taagtacagt agcttttcct ttttgttgaa
    21121 atcaaggttc accaattcat cgcggatgcc gcgcagttcg atggccgtat caatgcgggc
    21181 ctgttccaat tgcgtctgaa tcttctctat ttcggtttgg atggtactca ttttgttggc
    21241 caattcggtg cggtccaacg tagccacata atcgccttct ttgaccaacg acccctccgt
    21301 gaccaaatcg gtgatggtgg tttcccagat gccggcagcg ttcatgccct gtggcgcccg
    21361 gattttgaca gatcgcttgg cgctcagttc gccggtggcc gttacccgca catgaaatac
    21421 gtcttttttg actgtgtatt tcagtacggc agccgtttcc gtttcattcg tttttttgct
    21481 ccagaaggtg taaatcgcag agccgcaaag ggttaagatg agtgctgcga cacctattct
    21541 aacaatgttt ttacgagtca tttttttaca atgtattagc tttcaaagaa ataaattgaa
    21601 accaaaatcc tcctctccga cacttgaaag cgcaagttga ttgttttatt tcataaaacc
    21661 aagacattca atggttcttg agattagaga aaaaaaaatt taaaaaaggt actgcaatta
    21721 cgttgccgct ttaatccttt tgctccatct ttgtagcccc gataaaaacc cgtgctcgaa
    21781 cggcgcgact ttacgaggaa attatagcac ctgtctttcg gtcggagacc gacttgtact
    21841 aagttactga taccacgctg acttggctct tgcgagagag ttatttcatt attgaggcat
    21901 tttgatgcct ccaaaaattg cactggagaa tgatgagaga tcatgctacg gtatgggaca
    21961 gttgtctgca agtgattcgn nnnnnnnnnn nnnnacaaaa catggtttga accgatcaaa
    22021 cccgtcaggt tgcaagacaa cgcgcttaca attcaggtgc cgaacaagtt cttctacgaa
    22081 tggctggaag agcattatgt cagtctttta aaaagctcta tccgtaacga actgggcgac
    22141 cgcggccgtt tggagtatca gattttgatg ggtaccaacg agcgccccat cgctaccgct
    22201 cccgataagt ccggcccgtt cacgcctggg tcttttgaaa cggatgccat caaaaatcct
    22261 tttgtcattc cgggtatcaa gaagatcaaa gtggacccgc aattgaacgc ggcttatact
    22321 ttcgagtctt ttattgaagg agactgcaat cgtctggcgc gttctgccgg attagccatt
    22381 gccaaaaagc cgggcggaac ggccttcaac cctttagttg tatatggcgc caccgggttg
    22441 gggaaaacac atttggccca agccatcggc aacgagattg tagcaggtag caatgataag
    22501 tcggttctct atgtgtcttc cgaaaatttc acgaatcagg tcatcaacgc cattcggaac
    22561 aatgctgtcg gggatgtggt caatttttac cagcaaatag atgtgctcat catagacgac
    22621 attcagtttt ttgccaacaa attcaagatg caggagattt tcttcaacat cttcaatcag
    22681 ttgcatcaaa gcggccgtca gatcattatg acctccgacc ggcctcccaa agacctcgaa
    22741 ggcatcgaag accgccttat ttcccgcttt aagtggggac ttaccgccga tttgcaaacg
    22801 cccgatctgg aaacccgcat cgccattctc gaaaccaaaa tggcgcgtca gggggtggat
    22861 atgccgcaaa atgtgtcgga gtttatttgt tacaatatac agtccaacat ccgggaactc
    22921 gaaggggtac tcatttcgtt gattgctcag tcgtcgctca accgccggga aattgatata
    22981 gacctggcaa aagaagtaat tcggagcttc gttacacaaa tcaacaaaga aattacggta
    23041 gagtacatcc aaaaactcgt tgccgaacat ttcaaagtgc ccgtagaaaa gctacagggc
    23101 gaaaccagaa agcgctccat cgtcattgcc cggcagctct ctatgtatct ggccaaaaac
    23161 atgacagata aatcgctcaa ggccatcggt gagaattttg gaggaagaga tcacagcaca
    23221 gtgatctact cctgcaaaac ggtgcaagac ctgatggaga ccgatccttt tttcaaagat
    23281 accgtaaccg aacttgaacg caagatcaag ttgagcctca acgagaaagg ttaaaaaagt
    23341 ggtcattttt tgaaactggg accttatttg aaagaaattt tttctgcaaa aaagcgcggc
    23401 aattgtttgt ttgaaatgaa aaaccttcta catttgcatc cgcttttgaa acagctatca
    23461 aaagtcgaga cgattcatct cgttctcagt cggtgaagtg ggtgagtggc tgaaaccagc
    23521 agtttgctaa actgccgtac tcgcaagggt accgcgggtt cgaatcccgc cttcaccgcc
    23581 ataatttaac tacnnnnnnn nnngcagtcg aaccctgcaa aagaattcgg gatgtagcgc
    23641 agcccggtag cgcacctgct ttgggagcag ggggccccaa gttcgaatct tggtatcccg
    23701 acactgaaaa tgaagcactt acgacgattt ttgttgtaag tgctttttgt ttgtagcgca
    23761 gcccgatagc gcatcccggc ctaaggctgc gtcgcctcag cgcaaaagcc tgcctgcttg
    23821 cggaaattgt ttacttttgt ggcctgccaa atcatttttt ttatcaccaa aattcctttt
    23881 ttatgcatcg ctttttcaat gtgttgctgt gcatcgctgt gtgcgcaacg ttcttttctt
    23941 cctgcaaaaa aagcgcgccg cccgcctggc aacagttcat ggattgtgcc ggcgttgatt
    24001 gtactaccca ggtggctgcc gtgaaagacc tgtacctgca agacccgacc gtgctgtttg
    24061 aggaatttat caagaccgat gaacgcggcg aagaccattt cataggctgg ttgtacctct
    24121 tgcgcgacag cgtgctgatg aacccgaact ttgcttccga tgtagatcgc tcggaaatgc
    24181 aggcggccat tttggacaaa acccgccctt acgaaaatga cgccaaatac ggttcctggg
    24241 ccaagtctat tcttatggaa atagagccgc tgtctatctc acttgagatg caacccatca
    24301 cgggcactta tacctacgaa ttgccgaacg acggcggttc cggtgaaatc aaaatctatc
    24361 cttccgacga cggcacggtg cgctacgcca ttgcggtggt gggatctgca cccgctttca
    24421 atcagggcat gatggagggg aacgcgccac tgaaaggcaa tgtagttacc atcaccaata
    24481 cggaattcgg tggtaactgc gtcatcgaac tgacttttgc cnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgctcccgc
    24661 cgggttggag ggccactggg tcagcacttc ggatgtcaag tcggaggtag tcatcggcaa
    24721 cggcaagtat gtggaaatat atgacggcaa ggaggtttct tccacgccgt accgctactt
    24781 caacgcctgc ccggaagact gctcgccggt ggcgccgacg ccttgcctgc aattgatggg
    24841 gcaagacatt ctgtgctata cggttgtaaa ggtttctgcc aagcgcttag agttgtccat
    24901 gatcggcggc aggggcaata cgctggcttt tgtgccgaag aagtaagagg aactgagtcc
    24961 tccgaggccg cctggcatag agctggcagg tctatccggc aatagaaaga agacctgtta
    25021 ggggcgataa aaaataaagt ggtcattttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
    25201 catttggggc tttctcctcc tcggcgcctt gtgggcggaa gcgcaggagg cctgtacaag
    25261 caccgctacc gacagcatct gccggcacta tttcgagcga taccggcaag tgggcatggc
    25321 ggttggtatt gtacacggcg atgaggctta tcaatttgct gccggcgcca ccgatcacag
    25381 cgggcgcttt cccgtcaccg acagcaccct gtttcattta gcctcagtga cgaagctgtt
    25441 caccgccaca gccatcctgc aattggcaga agccggaaaa ctccgcctcg acgatgcgct
    25501 gacgaaacat attcctgagt tctccatgcg cgatgagcga taccgggata ttcagttgca
    25561 gcatttgctc acacattcct ccggactgat gtgggacaat aagctaaaaa attcgcccga
    25621 tgacgccggc tccatgccgc tgtttatcaa tcagttgaaa aagaaaaaat tagcctttca
    25681 gcccggcgaa aagatgtcgt acagcaccta cagcaatgcc ggtttcgact tgctggggat
    25741 ggttgtggag cgggtgtcgg ggcagtctta cgatcagtat atcccatccc ggatatggtc
    25801 gcaagcaggc atagccggcg ccacatttta ttacgaaacg gctgaaaaag cgcttttggc
    25861 cctgcctcaa aagctggagg gccgttccca aaaagtcagg atgctcaacc ggggcctaca
    25921 ttacgacagc ttagcctttt ccggtccctg gccctttcca accatcacgc acccggtgta
    25981 tggcgaagag tacgaacaca atgcgagcgg caatgccatt gcatcggcag cagaactgcg
    26041 cctgtggatg cggcatttgc tcgacatcta tcggcacgat aaaaaagacg gcgtattggg
    26101 ccggcagcgc ctggcagata tgtgggatac gcaccaatct gctaccgata gcaaggccaa
    26161 aatcggctgg ggctggtgga tcagcgatga gccgttcggg aaaatggtat ttcatgtggg
    26221 caacaacccg gggttcagtt ctatactctt gttgtatccc gaccacgatt tcggaatcgt
    26281 cgttttatgc aacagcaact acgcacagct cattgtctgg aaccgcttca ccgaagattt
    26341 ggcgcgggtg tggctggggc ggtgagggat ttgcaataca gattcattcc aactcattgt
    26401 gcaaatgact ccaagagttt tccagcaagt tacggatgga ttgccaatct tgttcagaca
    26461 ttcccattcc tttactgtcc cgcagcagca tagcctgcac gcgcaggttt gcgtactctc
    26521 tgaaaccagc ggcgggcatg ccatagaact cggaggcagt ggcgcacagc aatcgctccc
    26581 agtcctgcgg cgagaactgg tcgggttccc tgcgaacgat ccaccactcc aattcataag
    26641 cagacaccgt ttccggttgg aacggcttgt cgctcatctg gcgcagcgct gaaaaatagc
    26701 tgttcaggtg aggccatgcg cggccatatt cttcccggtt gctgcccgcc tgaaacaccg
    26761 cagcagcgcg agccgcatga aaagccagca agtagcttcg ccaatacggc gctttgaact
    26821 gcacgcgtat cagccgggcc aactgccgga acagccggaa atgattgcgt tcgtaatagc
    26881 tgcgccacat ctccgtttcc aaacgaccga cggccggcgc atcgaaacgc ctgaaatcag
    26941 tctgcacggg atacaggaga tcaacggcga tccagatgct gaatatggct gctgcgatgc
    27001 cgatgccgac gatgaacttc atggcctcag aggactttgt tgttggtgac attgtgcgga
    27061 aaaacaggac cgggcggcga ggggacggcg gcgcgttctg catcggaagc ggcttttctg
    27121 cggcgcaact ccaatttcag gctttcttct ccttttcgca tggcccattg gtggttggcc
    27181 gaaaagagcg ggcgcaacag ccatgtgagt tttttgagca gtggtttttc tgcgcggatg
    27241 cgccagtcat aaatgacttc acattgctct tcattcatgt ctctgaacgt ccattcgccg
    27301 gtgccggcaa agtcgccgat ggcttccaag gaaaacccga tggggaattg cgtctgcgtg
    27361 acgcgaaact tccattgcag ggtatagggc aagaagcctt tggtgtaaag cgccaccaat
    27421 ttgcccaccc cgccgggctg ccctttttcc aattgcttca catcaaggta aacgctgggc
    27481 caccacctgg ccaggtcttc cacggcttcc aaagtgcgat atacctctgc gctggtagcg
    27541 ttcacaaccc aacgggtgat gaagtggtaa gtgttctttt tatccgatac gggtactgag
    27601 ttgacgatgt tcatgttgca ggttttttac aatgaaagca gtatatccgc cagttctttg
    27661 gggtgggaca aaaacggtga aaggcaagtt cgaagaaata ccttccggat ttcagtatct
    27721 tcttatgata caaagatacc atcgcaacca cccggcgggc aatcccatga aaacaggctt
    27781 tttttctacc ggaaaagggg gagaggcgtt attgctgtac cactaattgg gaattaaaag
    27841 tccacaaagg taaaatttgt cataatagta cattcgcttt taaatgattt gtattttgtg
    27901 gcttattcca atttctgcac tatttcactt taaaatatca aaacatgaca aagcaaatct
    27961 tcgccgtatt agtaagcgcc gtactcttgt ttttgtggca attcctgtcc tggtctatgc
    28021 tgggcgtaca tcaggacgaa tacaagtaca cgcccaatca ggacaagatt ttggaagcat
    28081 taaatgccaa cctgagcgaa aacggagact attttcttcc cggcgtggcg cccggtactc
    28141 caatggatca ggaacaggct tttatggaaa accaggccgg aaaaccctgg gccaagatca
    28201 gctatcgcaa agcattcaat accaatatgg gcatgaacct gtttcgcgga tttgtagcag
    28261 atctggcggc cgcttttcta ttggtttggt tgctgatgaa ggccgaaaag cgcagtttct
    28321 ccagcgcgat gttaagctct ttagccgtgg gttttatagg gtaccttacc attccctatt
    28381 tagacagcat ctggtatgag accaaatcca tcggattttt gattgattcg gttgtccaat
    28441 ggggacttgt aggtgtttgg ctcggctggt ggttgccgaa gcgctgattt tcaaatgtat
    28501 agatgtcagg aagtaacttc gggttacttc ctgacttatc aagcgcgcgt caattcttag
    28561 tcagcggatt agcccccagt tgtattagca gccccagcat atctgcattg agccagagtt
    28621 cctgtgcttt accgttgttg aaataaaagg tgacggttgc cgataccttt atttttcgat
    28681 tgcttttggg tacgccctga aaagcagctc ccgtgtgcgt gccttccaat tggtggcgca
    28741 ccactacttt gtcaccttcc gatacaacat cggatacggt gaatgcagca tcagggaagg
    28801 actccaaaat ttctgtcatg gcttgccggt atcccttgct gtcgagaggt tgcggtcccc
    28861 agccgttgaa tcgggcgtct gccgcacaat attggatcac gccgtctaaa tcgtgtgtgc
    28921 gtaaatttgc catcattttc aaggccaatt ctgttgcttt gttgatcggg gcaggcagcc
    28981 cctgcatgag ttgtgcatta aacgctgcca aatcaaaaga tatctctatt ctgactgcct
    29041 tgccgtcggc gtcaaaagtc cagtattgca aaaaaggaag ctccacgcga ttgcccgtag
    29101 acggattgcc cataagcgaa ccggtgttgg tgccggagaa aatgcctctc acggcggcag
    29161 tcattttacc ttcggttact tcaagtactt tgtggctgat gtcgggaaat cccatacgga
    29221 aaccttcgcc cagttggcga taggcatcgc ggttgagcgg ggtaggcgaa agcggcagat
    29281 atactttcag atcggggctg agatacgtca cggctttgtc gaactggccg ttgtccacac
    29341 cggcccaaaa tgcagtaatc gaagttgcgt acttgcctcc aaaggcagtg aacggtaagg
    29401 taaacaggag cgcaaaaagc gcagcgcaaa aagaaaaatg ggttttcatg atgaatgaaa
    29461 ttttgataat gaatgaatta aatgattgca tggaggtgga aattcataaa aatgcccgtt
    29521 ccagagtact catgtctcca caggtttcat attgtacgac gcgaccgtca tctccgaaag
    29581 tccagacgga agactccggg aggtaaagtg tttttccggt aggtatgacc atcagctcta
    29641 catgtacatc gttggtcacc gagttgcctt cggctgcgaa gttgtgtggc tcgaatcggg
    29701 tcatttgtac agactgggaa acggcttcaa aaaagcgcaa aacgcctgcc tttccttcat
    29761 acgtcccacc gaaaggtacg atgtgtggat tggcgccgtg cttccataca atatcttcag
    29821 aacacatctc caatacagca gggatattgc cttgcaggaa gaggttgtag atgagctggg
    29881 cggcggctaa atttcgcttg gcaacttctg ccgattggtt ttcaaaaaca gcggtcattg
    29941 gtcattgatt tttgagtgaa aaaggcgcta tgtttttcag cgcttgaaca ctgcaaagat
    30001 gcgctcgcaa catcctcgaa atcaatcccg taaaaacagg cttttcatct cccgtaaaaa
    30061 ggggatttta tcctcgcgcc ttgtacagga agattaggat tccgtatctt cggaaaaaat
    30121 ttcggcacat gcgtacaggg atagccattt tgtgggtact gctttcggcg cttgggcagc
    30181 cggcaatgtc tcaattggac agcctgttgc aggttgcccg ccaatccaac agcgatactg
    30241 cccgggtctg ggcctggatg gaaattggaa aattatatct gcaatcgcag cccgatacgg
    30301 ccgagttata cctcaaccaa gccctgcata ttgctgaaaa agcgggtttt gaacggggtg
    30361 ttgccaagtg ccgcatcaac agggcttttg ccttcaacaa tcagggaaag tacaaaaagt
    30421 ccgtggacga ctgccttgtg gctgtgcctt tatgccggaa gctctatatg aagaaagaac
    30481 tggtagcagc ctacaacaat atgggcaatg cctgggatta tatgggcaac cgctggcaag
    30541 ccattgacgc tttttccaat gcactgtcag aattggatgc cgcaggtttg ccgccacatt
    30601 ttccgattgt agtaaaaaac aacatcacgc gtcaatacgc caatctgggt ttgcaccaaa
    30661 aagcctacga ttacagcctc cagtcttatg aggatgccat gttgctgggc gattcttctt
    30721 tggtggcttc ggcggtacat catctggcat ccgctgcttt gtcgttgggg cgcagcgagg
    30781 aagcgctgag ccattgccgc cgggtggaaa ggctggcccg gcaggtcaac gatcctatct
    30841 tgcttacttt tgcgctcaac aatatagctg tcattacatg gccggaaacc cctgctcagg
    30901 cagagagaga cattcgggaa gccctgtcgg tggcagagca aagcggggat ttgttcggaa
    30961 agatcggctc gttgtacgtc acggcgcgtt ttgcaatttg gaaaggtcgt cctcaagaag
    31021 ccagaaaatt ggttctcgaa gcgatggacg aagcgcgaaa agaaggtatg gacgatgagg
    31081 tagctacggc ctattcgctc ctctcagaca ttgcattggc tacgggcaat atggacgagt
    31141 accggaatat gcgtcgtcgc ttcaatttta tgagcgatac ccttatgaac aatcagctcg
    31201 tggtagctac ccaagagttg gaaacccaat atgaaacagc ccaaaaagaa cagcagattg
    31261 tggcgctttg gcagcagaag gaactccaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31321 nnnnnnnnnn natcggtctt gccgctgccg caacggctat gctggtactg gcggctttgt
    31381 tgtggaatgt gttccaaagc cgaagaaaaa tcctggggca ggaaatcctc attcagcaac
    31441 aaaaaatcaa agacctggag caagaacgcc agcttggttt tgccgatgcg gtgatgcagg
    31501 ggcaggaaaa ggagcgctcg cggctggctc gcgacttgca cgacggtttg gggggcatgc
    31561 tttccgggat aaaacaggct gtgtttagtc tcatcgggca ttctcctaag gaggccggaa
    31621 cggccgccgg acttcataaa atcgttcagg atattgatca gtctatccat gaattgaggc
    31681 acatcgcccg caatctcatg cccgaagcct tggtccggtt cgggttgcag gatgcgcttc
    31741 aggattactg cgaccatttg caacaaccgg acaagttaca cattcatttt caatcatttg
    31801 gtatgaccgg acgcctggcc cgcgagacag aagtggtgct ctttcgcatc gcgcaggagt
    31861 tgctcaataa cgttgttcgg cacgccggtg cttcggaagt gctggtgcaa ctactccgcg
    31921 acggtcaccg cctcagcctc acggtagaag acaatggacg agggttttat acccccaatt
    31981 tggcgaaagt agagggcgtc ggatggttca atattcgttc cagagtcagt taccttagcg
    32041 gcaatctcga tctgaaatcg gcgcccaacg aaggatgttc cgttcacata gaaattcctt
    32101 taccatgatc gaagttttca tcattgacga tcacccgatg gtggtggcag gtatccaggc
    32161 gttgctcaac cactctgaag tagtgcgaat ttgcggcgcc gcccatgacg caggcacggc
    32221 cttggaaatg ctcggaacga gtactgcgca agtggttttg gtggacatta acttgcccga
    32281 catgaacgga atagatctgt gtgttttgat gagaaaagaa tttccaaaac ttaaaatact
    32341 gggtctgagt acattcaaag agcgcagcta catcacccgt atgatggaag ccggcgcatc
    32401 ggggtatttg ctcaaaaatg cctctaagga agaactcgaa gatgccgtag tggctgccaa
    32461 taaaggccgc gcatatctaa gcatggaagt agccgccaca ttggctgttg ctcaaagtgt
    32521 tccatcggcc atcccaatgc tcaccgccag agaaaaagaa gtactgcatc tcattgcaga
    32581 ggggcttacc aatcaggaga ttgcagataa actcttcatc agcatcctca ctgtggacag
    32641 ccacaggaaa aatctgctgg ccaaatttgg agtaaaaaac actgccgcca tgattaaaac
    32701 ggcgctggaa ctcaggttga ttgctttttg aagcaaccac acgaggttca tcgctttcgc
    32761 gggattctct acaaaaactc agtatcattg caccgccaat ttacgcgccc aatgaacttc
    32821 caacaattag aatacatcgt agccttgcac gcccatcgca acttcaccgt cgcggccggg
    32881 cactgcttcg tcacgcagtc caccctcagt tctatgattc aacgcctgga ggaagagttg
    32941 gacatcgtca ttttcgaccg cagcaaaaag cccgttgaag ccacccccgc cggccttgag
    33001 atcatcgccc gcgcacaaaa aatcctggcc gaaaccgctg cactgcgaca ctatgccaca
    33061 gaactgcatc agggcctttc cggcgaactg cgcatggccg tcatacccac gctggcgccg
    33121 tacctgttgc cgctctttgt gggcaatttt cttcgcagtt acccagatat tcac
//
LOCUS       DAORLF010000006        29034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_893_length_29034_cov_2.167777, whole genome shotgun sequence.
ACCESSION   DAORLF010000006 DAORLF010000000
VERSION     DAORLF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 29034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29034
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_893_length_29034_cov_2.167777"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="PK198_01345"
     CDS             complement(<1..2029)
                     /locus_tag="PK198_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37405.1"
                     /translation="MIWKSAVRRWSFVAVAALYALLLFGRLTAQNTITEKAPAYLQGL
                     PVIRNYPPEVYAAHNQNWAAVQDRRGVMYFGNSYGVLEYDGVSWRLIKLGGRRIARSL
                     AIDAGERIFVGGYGDLGYLAPDEKGQLKFVSLMPEMEERHRNFTDVWQTISTPDGVYF
                     LSSQYIFRWDGRKMHTIRAESAFQSAYYIYNQLFVFQTDKPVRHLVSNALKPVPAAAS
                     FSASDITAMIPLQEQQERNMLIATRGRGIFQYDGQSAPTPFLTEAQSLLAEGQVFSAI
                     ALSHGRYAFGTMLRGVVITDARGRLLQHLNKASGLLDDAVLCLYEDRQQGLWTGLQTG
                     IARAETGASLSYFREREGMEGSIWDMARHEGRLWLATIMGLYYLDDSPAVVRTPQRIR
                     RMPGVSPQCWSLAPFGSSLLAAAFDGIYEIKNGQSRLIFKEFSFAVHRSQQDSNRLFV
                     GLRSGVKTLYYTNGQWKDEGRLEGVEHEIRHFYEAPDGRLWLIGYFEGPVLADFSKGF
                     SSQAPLKRYDTKHGLPPADRIIGFPTDRGLRFATLQGVFAFDEARQRFYRDSTLVEGL
                     SDPALPLFHAARAPEGHLWLTADNNAQSGMALWRADGKYEWRPNILPRIAAMQVFTVY
                     PDPQQPGIVWIGGTDELVRHDAAIPARTAAAYPALIREMTLNGDSLLYAGTG"
     gene            complement(2061..>3563)
                     /locus_tag="PK198_01350"
     CDS             complement(2061..>3563)
                     /locus_tag="PK198_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF37406.1"
                     /translation="FYLGAGEEGVLFYGEWQVDPNTTFPATFAVGRYKMNERKSEEVM
                     GGLTRFDISADGKKILYGTLDNTWGIVDASGTPKPGDGKLNLSEMQMKVDPLAEARQI
                     FREAWRYQRDFFYVENVHGLDLDWAWKSYSPWVEHVRHRSDLNYILDILGGETSIGHS
                     FVGGGDYPDVDRVPAGLLGANYEVVNGKYRFAKIFNGENWNPTLKAPLTQPGVDVKQG
                     DYLLAVNGVPLDGATNIFAHFDRTAGKQTRLLVNSQPSTDGAREVVVVPVSDESGLRQ
                     LEWVESNRRKVDELSGGRLAYVWVPNTGGEGYTYFNRWYFAQKDKKGAVIDERFNQGG
                     YIADYIIEVLSRTHYGYFNNPVGDKTPMLTQDAAIHGPKVMLINEMAGSGGDMLPFMF
                     RFKKIGPLVGKRTWGGLVGIWDVPGLLDGGYITAPRGGFFNLQGEWDVENIGVAPDIE
                     VEMEPKLVNQGRDPQLEAGVRQALEMLKTQELKLIPQPVDPVRAKRPRGR"
     assembly_gap    3564..3599
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3600..5307)
                     /locus_tag="PK198_01355"
     CDS             complement(<3600..5307)
                     /locus_tag="PK198_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HRF37407.1"
                     /translation="MNTRLSLFATTALLIVMGIEGFSQETRLLRQATISANHIAFVYA
                     GDVWTAELDGKNPRRLTTHPGTEGNPYFSPDGKWIAFSAEYDGNNDVFVAPVEGGEPR
                     RLTWHPQADIAKGWTPDGKHVLFASGRENAPYPSPDRFWKVSPDGKGLEQALPIPRVW
                     EGSFSPDGKRFAYQMIMPWENEFRNYRGGQNNPIRIIDLANFEQITLPFDGANDREPQ
                     WVGDEIFFLSDRDFAMNIWSYNLNTKQLKQVTKFRDFDCKSLRSGAGKLIFENGGRLY
                     TLDAKGGEPKKLTITVEGDFPWARPHWEKVGAGIAEYSLSPGGKRVLMSSRGDVFSVP
                     AEKGDIRNLTTSSGVADRSPACSPDGEKIAYFSDESGEYQLFIADAFGKVQKKIALAN
                     PTYYYTPVWSPDSKYLGFGDADRNLWYLDIATGKATLVDNEGSAHPERNIAPAWSPDS
                     KWIAYSKRLGNEFNTIFVYSLEQKKAMQFTDGMSNCKAPVWDKSGKYLYFLSSTDFAL
                     GVGWLDLSSYSRVSTQNVYVAVLSKKEASPLKPLSDEEEKPDTTKKDTAKIKVNIDFD
                     GLG"
     assembly_gap    5427..5625
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5776..6555
                     /locus_tag="PK198_01360"
     CDS             5776..6555
                     /locus_tag="PK198_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF37408.1"
                     /translation="MDASILYQFSEGVATLTLHRPQVYNSFNREMALRLIGHLEACAA
                     DPAVRAVLITGAGKAFCAGQDLQEVVDPQGPSLTNILKEHYNPIILAIRRMEKPVICA
                     VNGVAAGAGANIALACDITLAAASASFIQAFSKIGLIPDSGGTFFLPRIIGFQKALAL
                     AMTGDRINATDAELLGMIYKVLPDEYLEEESKRLALTLAQMPTAALGFTKRAFNASMT
                     NNLETQLQLEETLQTTAGQTEDFQEGVRAFIEKRKPVFKGK"
     gene            6552..7646
                     /gene="mtnA"
                     /locus_tag="PK198_01365"
     CDS             6552..7646
                     /gene="mtnA"
                     /locus_tag="PK198_01365"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRF37409.1"
                     /translation="MSKLLTTIRLHPANERVVQVLDQRRLPFEEVMVDLHTPEDAAHA
                     IREMLVRGAPLIGVTAAYGMYLAALHEPAASVESYYQQQKSMLAATRPTAVDLFAALD
                     QAAQSILQAGSLAERQQQALAFAQQCAADSIDACRRIGEHGLPLIEAIARQKNGAPVN
                     ILTHCNAGFLACIEYGTATAPIYLAQERGIPVHVWVDETRPRNQGARLTAFELGRRGV
                     PHTLIADNAGGHLMQHGLVDLVITGTDRTTRCGDVANKIGTYLKALAAQDNGAPFYVA
                     APSSSIDWNIRDGLREINIEQRDDRELRYIEGWAEGRIQEVLLCPEGSAGANFAFDVT
                     PARLVTGIITERGVCAASEDGLTTLFPEKR"
     gene            complement(7677..>9211)
                     /locus_tag="PK198_01370"
     CDS             complement(7677..>9211)
                     /locus_tag="PK198_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF37410.1"
                     /translation="GGGKIGAPATPAIPRAAESAIERTAAQLQQARRPVLVIGSGAML
                     LPQEIVALTEALPRLGIPVYVSGMARGLLGREHPLLHLHERKKALREADLIILAGVPS
                     DFRLDYGSHLSRKAVKIVVSRHREELHKNLRGHRAILGDPGRFLIRLSEISAPRPEWD
                     EWKSQLQDWQAAREQEIARQAGATVGQGINPVTLFMVLEQMLPPNSILIADGGDFAAT
                     ASYTLRPRQPLGWLDPGVFGTLGVGGGFALGAARRYPNHYIWIIYGDGSAAYSLAELD
                     TFAKNGFKVCAIIGNNGSWEQIARDQVTFLGAATATTLPRSDYHRIAEAFAAACEREL
                     ALQAFEWIIWKPLPGRCSAPFSIWTKGFLTSSMPILAALILGRGVFRCSFGDPLIITD
                     KNKDMETNGNPFLNIKAELSPYFSIMGQASDTILDAEVSSYPIFIAATQEVPIGVLLM
                     RADNGWQVNASTLEELVARQVVAQDHVADFRMVYKDPRQYFCVFAVKDEGSNFLFIPR
                     EG"
     assembly_gap    9212..9257
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9258..>9538)
                     /locus_tag="PK198_01375"
     CDS             complement(<9258..>9538)
                     /locus_tag="PK198_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRF37411.1"
                     /translation="IGGATATLLRGRGALQDIDQMALVRPHVKWALRVRRLSQLAGAL
                     HKALHIARSGVPGPVFVEIPVDLLYDESLVREWYAQKTSRKNTLLEKLT"
     assembly_gap    9539..9611
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9612..9821)
                     /locus_tag="PK198_01380"
     CDS             complement(<9612..9821)
                     /locus_tag="PK198_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRF37412.1"
                     /translation="MNGGAITAQVLKAHGVERIFTLCGGHISPILVEGEKAGLRIVDV
                     RNEASAVFAADAAARLSGIPGVAAVT"
     assembly_gap    9900..9911
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9991..>10253)
                     /locus_tag="PK198_01385"
     CDS             complement(9991..>10253)
                     /locus_tag="PK198_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37413.1"
                     /translation="AAWQSNPLSGKTNMEDGTQKAQAFPYGAGRSSVVQTVLLNSLLK
                     LFPCRGMTREVSCCSIHCRFPIRGLLPGCSEKRYVKDKVPEI"
     assembly_gap    10254..10263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10579..>11103
                     /locus_tag="PK198_01390"
     CDS             10579..>11103
                     /locus_tag="PK198_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37414.1"
                     /translation="MKKILVILMLLSSALGLRAQGSISDPFFNHGQARSLNSRIIAPL
                     QPVPGFVNQAIAGMRVSNNRNLVRLAHLKDDYGYAVWDQAKTFTAAGRQTALIPMVRP
                     EDDAITGILVAVKDGATFRYGVMDTGTPFPYASAATGTPGMPGMRDVLALSVLFNRTL
                     FGEVDCDLVQALRAL"
     assembly_gap    11104..11128
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11129..11799
                     /locus_tag="PK198_01395"
     CDS             <11129..11799
                     /locus_tag="PK198_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37415.1"
                     /translation="GQTAGSPKSCYVQTTYAGQNCDHIYGTYPDGTMQYLYSDCYDIY
                     NYSFICDTDSADNDDGGSGGGSGGSGGGNGSSGPGEGDNESFCESLDVHKCTTTRVLS
                     TVPSGIHGSCSIVCRLSGCGFQSNVFLQPNGIAGCGFSIGTGSGHGISISLDFTVSIQ
                     NLYTNSYINSVSTLDSDCRVSSICGDFFCSYDVVISWIGIPYIWERNSAELSNICYTC
                     RNCQ"
     gene            11820..12149
                     /locus_tag="PK198_01400"
     CDS             11820..12149
                     /locus_tag="PK198_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37416.1"
                     /translation="MKNQSFFIFALLAFILVSACKTSENNRESVLDAKRMEVEALVAK
                     YSSENLAGPVNWQQFNAALKDEAVLEQMNTAQLEACLKSMTAYSNNIILENRKNGCDQ
                     LQEQVKS"
     gene            12423..12674
                     /locus_tag="PK198_01405"
     CDS             12423..12674
                     /locus_tag="PK198_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37417.1"
                     /translation="MIISFFLLLFTALDCKTQEKQEAIINNKRVETEAILNKHLQSME
                     SSVTVNYDQFRESLSHDELIAYIDLNQLGTKRTTTKNSP"
     gene            complement(12784..13257)
                     /locus_tag="PK198_01410"
     CDS             complement(12784..13257)
                     /locus_tag="PK198_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37418.1"
                     /translation="MKKWIFSIALSLIALAALQAQPRPERGGGERVKAMRTAFITNKL
                     NLTPEEAQQFWPLYNQFEAEQKKIRDKFVPAKDLMSMSDAEAEKFLNNQLDMEQELLD
                     LRKDYLQRMKKVLPVRKVAMLPRLEREFREELLDNLQQMRANRGGAAPQRPAPRN"
     gene            complement(13279..>13574)
                     /locus_tag="PK198_01415"
     CDS             complement(13279..>13574)
                     /locus_tag="PK198_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37419.1"
                     /translation="PSYVVGFATVVLALVAAFWIWSPTTNDAGASLASLTEEETLAYI
                     ENNIEDFDTDLLAQTIAGNTSWTGASGLSGEDADRLLEELLLELEADSLEKLF"
     assembly_gap    13575..13584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13585..13810)
                     /locus_tag="PK198_01420"
     CDS             complement(<13585..13810)
                     /locus_tag="PK198_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37420.1"
                     /translation="MSEHTNKEAREELAGLSPLLSRIRQSESAIEAPENYFRDMQQDV
                     LWRLKTEVTAAPAPARPTAWERWAAVFQVFK"
     gene            complement(13815..14363)
                     /locus_tag="PK198_01425"
     CDS             complement(13815..14363)
                     /locus_tag="PK198_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF37421.1"
                     /translation="MSISTPDEQILALMRSNGGMERGLKMLMDKYQAPLYRQLMRMTG
                     NHDDANDLLQNCFIKAYRHIGQFEGKSKLSSWLYRIAANEAITFLNSVKRRATDSMQA
                     HEHLSASMQADAPIDADALQERLRSAISALPEKQKLVFSLRYYEEMPYEEMSKTLGTS
                     VGALKASYHHAVKKIEDFFHEK"
     gene            14618..16801
                     /locus_tag="PK198_01430"
     CDS             14618..16801
                     /locus_tag="PK198_01430"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRF37422.1"
                     /translation="MGQNLLYVVPVLGVLGLLYVAWRSAWVSKQEVGTERMHRIAKAI
                     SEGAMAFLKAEYRVLAVFVAVVAVLLAVSADAQKSSWLVGVSFLIGAISSALAGFIGM
                     RVATKANVRTTNAARTSLGKALEVAFAGGSVMGMGVVGLGVLGLSILFIVYGNMFGTG
                     TAEDVTRVITVLTGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHPLN
                     PATIADNVGDNVGDVAGMGADLFESYVGSIVGTMVLGAAFIGLTEFQGAALQMNGLNA
                     VLLPLVLAGVGIVTSIIGTFFVKVKEGGNPQKALNTGELLSSAVMLAATWFVVKWMMP
                     ATWAFNGTEYSSTGIFFAVVIGLLAGLGIGMITEFYTGTGTRPVKSIVNQSVTGSATN
                     IIAGLGVGMQSTAVPILILAAGIIGAYHFGGLYGIAIAAVGMLSNLGIQLAVDAYGPI
                     ADNAGGVAEMSELPKEVRARTDKLDAVGNTTAAIGKGFAIGSAALTALALFAAYMQVA
                     QVNVIDVSNPQVMAGLLIGSMLPFLFSSMAMDAVGKAAMDMIQEVRRQFNDIPELKKA
                     LDVMRKNDGKEMDEWSEADRNVFHAADGKAEYGRCVEISTKAAIRQMILPGLLAVLSP
                     VLIGFLGGKEMLGGLLAGVTSAGVLMAIFQSNAGGAWDNAKKMFEGGVDINGKTYYKG
                     SEPHKAAVVGDTVGDPFKDTSGPSLNILLKLMSIVALILAPLLAG"
     gene            complement(16910..18142)
                     /locus_tag="PK198_01435"
     CDS             complement(16910..18142)
                     /locus_tag="PK198_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRF37423.1"
                     /translation="MLLRLWFFAGLFAGVSMTALSQSPDLILRRNVDRIDIPFEYENN
                     FIIVNVILNDLLPLRFIFDTGAEHTILTQRDFTDILQVQYGRRLPLMGSDLSVELYAF
                     LAIGVKLNVGGLIAPNRTILVLEEDYVNFKEFAGVDVHGIIGSDLFRRFVVRINYKQQ
                     IISLINPVHFKPPGNRYIEIPVEMIRNKPYIKASTIIAGKQVNDYKYLVDTGAGMAIL
                     IYTNTDPSLHLPPQVVRSRLGMGLGGNIEGFLGRIGYLRFADMEMKGVITNFQDLPFP
                     DDTLRTQQRNGIIGNQILNRFVMIFDYPNSKLYASPTSRAKAVFKSEKSGMLLAASGK
                     RLDTFTVFDVLPGSPAASAGIQVNDQVRSVNGWPASVHTLEELTNKFYGKAGKKMKVV
                     IERNGVRMLKVVVLRELI"
     gene            18436..21393
                     /locus_tag="PK198_01440"
     CDS             18436..21393
                     /locus_tag="PK198_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37424.1"
                     /translation="MKLRVNYFSQGLLLVAFMLLGTWAMAQRTISGIVTDAATKEPLI
                     GANVLVVGTSAGTITDIDGTYSVSVPAGATELEFSYTGYASQKVAIGASSKVDVSLSA
                     GRLLDEVVVVGYGTVRKGDATGAVVAISDKDFNKGVIASPEQLLQGRAAGVQITAASG
                     EPGAGVNIRIRGTSSVRAGNNPLFVVDGVPLDGRDDSAGGTDFGAGSSSARNPLNFLS
                     PEDIETMTVLKDASAAAIYGSRGANGVVLITTKKGKVGTSNATFSAQTSVSNVLKRYD
                     LLSADEYVDAAVKAGANAQAVNFGGATDWQEEIFRTGIAQNYNFSYGGGNQKTLYRFS
                     LGYLDQEGVVENTALRRLSGRLNASHKMLNDKLEFSVQMTASRLNNDYAPITNNAGFE
                     GSLIGAALQANPTRRAVNADGTFPQSVDFRNPLALLAYIDDHGTTSRVLANIGATWHI
                     ASGLSYKLNVGVDNAESVRRTGISPKLAFNDIQGQGRAIVDNRYLSSRVVEHTLNFKK
                     AMGSSTIDAVAGFSYQEFQNQGDWVQARFFVTDLISLVDNLDGVNAQDNNNAYRAASD
                     RSVEELQSYFGRVNYSIGDRFIFTGTVRADGSTKFGPNNKYGIFPAINGAWRLSEESF
                     IPEAFYDLKLRAGWGITGNQEFPGNASKAILRANNAGVFDIVQFPNPDIRWESTTQTN
                     IGLDFGFAGGRLTGSLDYFNKVTKDLIFLQTFAQPAPGNATQFFNLDGDVVNSGVELT
                     LDARVVDKKSFNWQVLFNAATLKNEVRDLPTFVNTGAINGQGLTGAYAQRVISGSPLF
                     AFYLREFGGFTNEGLGIYPNGDLVTYAGSPIPTFTSGLTNNFTFGNLDLSIFFNGVFG
                     HYIYNNTANPIFLKGNLRNGRNVSRDLANSPESPNNFGEASTRFLEKGDFVRLQNLTV
                     GYTFNTGNWKGISSLRLYLTGQNLLLFTGYTGFDPEVNTDKSLNGIPSFGIDYTAYPS
                     ARTISLGLNVGFN"
     gene            21413..22960
                     /locus_tag="PK198_01445"
     CDS             21413..22960
                     /locus_tag="PK198_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF37425.1"
                     /translation="MRISKFFILLPVVALFAIMPACTNLEDIVEDGRGEDVVDNVLPL
                     PPDAAAALRGVYDQVTGLASQDNTFALLEHSSDEMMGPTRGTDWSDFGVWRQLHTHDW
                     DASHSFVLSGWNQLNSGVFQATRALAAAGSNARVAAEARFMRAYFMYLVTDLYGSVPF
                     REATEGILTIPKVFTRSEATDFIIADLTAAMPNLPNYQDVNPGVANKDAANALLAKVY
                     LNKAVFTAANPAGPYTFAAPDMQKVIEHADAVINSGRFQLTPYWDNFTADNSTKSREM
                     IFAIVNSPGNSRGNLQSRHRMTTHYNQNPSGWNGFTTIADFYNKWSASGSGDVRKSAA
                     IPGVTDVSGLRAGFLEGQQKDQNGNNLTDRGGNPLAFTVDVDLLYSNERQGVRCIKYP
                     PDFTNVDNPGNDYILLRYADVLLMKAEALFRTGNAGGARDIINQIRTHRGATAITATL
                     TADNILDERGYELYWEGWRRNDQVRFGQFLKAWYAKPQSNERAVLFPIPQIALDNNPN
                     LKQNPGY"
     assembly_gap    23099..23225
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23226..26422
                     /locus_tag="PK198_01450"
     CDS             <23226..26422
                     /locus_tag="PK198_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF37426.1"
                     /translation="ELSLSGEFDVFRYRNYYNGGGVAIGDINNDGRPDVFLTSNQGEN
                     RLFLNKGNFAFEDITTKAGVGGTRAWSTGVAMADVNGDGLLDIYVCNSGDVKGDDKEN
                     ELFINNGDLTFTEKAREYGLADKGFSTPAVFFDFDRDGDLDCYVLNNSFRPISTLNLR
                     NLRPFRDEEGGDKLYENQDGRFVDISEKAGIYGSVIGFGLGVTVGDVNMDGWPDIYIS
                     NDFYERDYLYLNNKNGTFAEALTEAMPHTSNFSMGADMADINNDGLPDIFVTDMLPQD
                     LQRIKQTTNFTTYDQYQLTLRNGFHHQAMRNTMQLNNGNGTFSDVAQVLGVHATDWSW
                     GALIADLDNNGHKDLFVCNGIYKDVTDQDYVNFLANDENLAAAERGEKIDFKRFVDLM
                     PSTKVHNYAFANQGGLRFEDRSTEWGFALPTHSNGAAYGDLDGDGDLDLIINNVNEKL
                     LVYDNRSNSMSENKSLKIKIQGEGKNTFALGSKVFVYAGGQMMYYENMPMRGFQSSMD
                     YEMVIGLGAAAQADSVVVFSPYRKRWTSGGPVQAGTNLTIDFSKAAGCTDADIPSPKP
                     AAKPLLAVANAMFGESRPRHTENTYIDFDRDKLLYHALSTEGPALAVGDVDGDGADDF
                     YLGGAHRQAGQLWQYRGGKYVRVRVPQFEEDQVFEDIAAVFADVDGDGDLDLIVAAGG
                     SEVLYESANLSSRLYRNESAGGQIRFVRDDAALPKIRQVMSCVVAFDFDNDDDVDLFF
                     GARLHANGYGLPASGYLLENDGKGKFTDVTVSKGAPFRQMGMITGAAAADLDQDGRQD
                     LVIAGEWMPLTVCYNKNGAFPASETTIISGAGWWQNVRIADWNGDGKLDVLGLNLGKN
                     SRFQGSENAPLRLYVKDFDKNGALEPIFCLQEKGKYFPMALRHDLGKELPMVKKKFNS
                     YKEYMDKDVQAVFGSDLDDALMLEAGHFETTLFLQTSARNWQQQALPEEAQFSIFYDA
                     LPEDFNGDGLPDILLTGNLSAVKPEVGAYNASYGTLLINDGKGHFAHWPAWKSGLSWK
                     GEARAVRTLRLPGNRRVALCAFNNGPVEAVELPR"
     gene            26528..>27643
                     /locus_tag="PK198_01455"
     CDS             26528..>27643
                     /locus_tag="PK198_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF37427.1"
                     /translation="MRPYQSLSLFSLVAILLLSHCAGKKEAPRFELLGSDKTGIRFQN
                     KLVPTGDMNIFNYMYFYNGGGVAVGDLNGDDLPDVYFTSNQEENKLYLNKGDFKFEDI
                     TQKAGVAGDPGWTTGVTMADVNGDGRLDIYVSQLGQHLKFRGRNQLYINMGNDPDGVP
                     VFENQASQYGLDLVGYATQAAFFDYDLDGDLDMYMLNHTVHRNGTFGYMKDLRYKNHP
                     TAADRLLRNDGGFFTDVTKESGIYSSVIGFGLGISTADLNLDGYPDVYVGNDFHEDDY
                     LYLNNGDGTFREVLRESMQHTSRFSMGNDVGDINNDGFSDIVSLDMLPIDPVMLKMSA
                     AEDPIDVYRFKLNYGYAHQFARNTLQLNRGAVPGDDPL"
     assembly_gap    27644..27674
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27675..>29034
                     /locus_tag="PK198_01460"
     CDS             <27675..>29034
                     /locus_tag="PK198_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF37428.1"
                     /translation="GVRFSEIGLQAGVAATDWSWSALFMDMDNDGWNDLFISNGIQRR
                     SNDMDYINYVVADSIQAQLADEVISDRNLKIADLMPEIKLPNFAYRNNGDLTFSDASA
                     DWGFELPSFSHGTAYADFDNDGDLDLVINNVNDEASLYRNLTKESACQDCNHLKISFK
                     GNAPNHWGLGAKVIVNTDKGTQLYEVSATKGFQSATDTRLNIGLGNAAKVQELTVVWP
                     GGAFQTLRESPANQTITLKQADATGSFDYAAYRNRGVSGAPFITVMAAEKLGLDSVHR
                     ENDFVEFNREALIPHAVSTEGPALAVGDVNGDGLEDVYQGGARRQAGILNLQQPDGRF
                     LPSVQPAFLQDSIWEDVDAAFFDADGDGDLDLFVASGGNEYNLGDAPTLPRLYLNDGK
                     GNFERSLQALPKEVALTASSIAVNDFDNDGDMDLFLGARATPWRYGEVPRSFLLRNNG
                     KGL"
BASE COUNT         6524 a         7847 c         7512 g         6582 t
ORIGIN      
        1 cgccggtgcc ggcatagagc agcgagtcgc cgttcagggt catttcccga atcagggcag
       61 gataggccgc tgccgttcgg gcgggtatcg cagcatcgtg ccggaccaac tcgtctgtgc
      121 cgccaatcca cacaatgcca ggctgttgtg ggtcgggata caccgtgaaa acctgcattg
      181 ctgcaatgcg gggcaaaatg ttgggccgcc attcgtattt cccgtcggcc cgccacaagg
      241 ccatgcccga ttgcgcattg ttgtcagcag tgagccaaag atgcccttcg ggagcgcggg
      301 cggcatgaaa cagcgggagg gccggatcgc tcagaccttc tacaagcgtg ctgtctctgt
      361 aaaatcgttg ccgggcctcg tcaaaagcaa agacgccctg caaggtggca aatcgcaggc
      421 cccggtcggt gggaaagcca ataatgcggt cggcaggcgg caggccatgc ttcgtatcgt
      481 agcgtttgag cggcgcttgg gaggaaaaac ctttggaaaa atcggcgagt accgggcctt
      541 caaaataccc gatgagccaa agtcgcccgt cgggagcttc ataaaaatgg cggatttcgt
      601 gctctacacc ctccagccgc ccctcgtctt tccattggcc attggtgtaa tacagggttt
      661 taacgccgct gcgaagcccc acaaaaaggc ggttggagtc ttgctgcgaa cggtgaactg
      721 caaagctgaa ctctttgaaa atgagccggc tctgtccgtt tttgatttca taaattccat
      781 caaaagcggc tgccagcaga gaggaaccaa agggcgccag gctccagcac tgcggcgata
      841 cgcccggcat tcggcggatg cgctgcgggg tgcgtaccac tgcggggctg tcgtccaaat
      901 agtacaagcc catgatggtg gccaaccaca aacgcccctc gtgccgcgcc atgtcccaga
      961 tactgccctc catgccttcc cgctcgcgga aatagctcag cgaagcaccg gtttcagcgc
     1021 gtgcaatgcc ggtttgcagg cccgtccaca agccctgctg ccggtcttcg tacagacaca
     1081 acaccgcatc gtcgagcaag ccggaagctt tgttcaagtg ttgcaacagg cgtcccctcg
     1141 catccgtgat gacaacgccc ctgagcatgg tgccgaaggc gtaccgccca tgcgacaggg
     1201 caatagcgct gaaaacctgc ccctcggcga gcaggctttg cgcttccgta agaaaagggg
     1261 tgggcgcgct ttggccgtcg tattgaaaaa tgcctctgcc gcgggtggcg atgagcatat
     1321 ttcgctcctg ttgctcttgt aagggaatca tggcggtgat gtcggaggca ctgaacgagg
     1381 cggcggcggg aaccggcttc agagcattgg aaacgaggtg ccggacgggt ttatctgttt
     1441 gaaaaacaaa gagttggttg taaatgtagt acgccgactg aaaagcgctc tccgcccgta
     1501 tcgtgtgcat tttgcggccg tcccacctga aaatatattg cgaactgagg aaatatacgc
     1561 cgtcgggcgt ggagatggtt tgccaaacat cggtgaaatt gcgatgcctt tcttccatct
     1621 ccggcatcag agaaacgaac ttcagttggc ctttttcgtc gggcgccaga tagcccaaat
     1681 cgccgtagcc tcctacaaaa atgcgctctc cggcatctat cgccagcgag cgggcaatgc
     1741 gcctgccccc caatttgatc aatctccacg acacgccgtc gtactccagc acgccgtagc
     1801 tgttgccgaa atacatcacg ccgcgccggt cttgcacggc agcccagttt tggttgtggg
     1861 cggcatatac ttcgggagga taattccgaa taacaggtaa accttgcaag taggcaggag
     1921 ccttttcggt aattgtattt tgtgccgtca ggcgaccgaa caaaagcagt gcatacaagg
     1981 cagccactgc cacaaaactc catcgtctta ccgctgattt ccagatcatg gccaattgat
     2041 tctccttcct gaacttctcc tcatctgcct ctgggccgtt ttgcgcgcac cgggtcgaca
     2101 ggttgtggta tcagcttcag ttcctgcgtt ttcaacattt cgagggcttg tctgacgccg
     2161 gcttcgagct gcggatcgcg cccctgattg actaatttag gctccatttc cacctcaata
     2221 tcgggggcga cgcctatgtt ttccacgtcc cattcgcctt gcaaattaaa gaaaccaccc
     2281 cgtggagcgg tgatgtagcc gccgtccaga agacccggca catcccagat acccactaag
     2341 ccgccccagg ttcttttgcc gacgagcggg ccgatttttt tgaagcggaa catgaaaggc
     2401 agcatatcgc cgccactgcc ggccatttcg ttgatgagca tcaccttggg gccgtggata
     2461 gccgcatcct gcgtgagcat cggcgtttta tcgcccacag ggttgttgaa atagccataa
     2521 tgtgttctcg acagcacctc gatgatataa tcggcgatgt agccgccctg attgaatcgc
     2581 tcatcaatca cggctccttt tttgtccttc tgagcgaagt accagcggtt gaaataagtg
     2641 tagccttcgc cacctgtatt gggcacccaa acataggcaa gccggccgcc ggagagctcg
     2701 tctactttgc ggcgattgga ctctacccac tcgagctgac gcaaaccgct ctcgtccgaa
     2761 accggcacaa ccacaacctc ccgagcgccg tcagtactcg gttggctgtt caccaacagg
     2821 cgggtttgct tgcctgccgt ccgatcgaaa tgtgcaaaaa tattggtagc gccatccagg
     2881 ggcactccgt tcaccgccag cagatagtcg ccctgcttca cgtccacgcc aggctgcgtg
     2941 agcggcgcct tgagcgttgg gttccagttt tcaccgttga aaatttttgc aaagcgatac
     3001 ttgccgttga ctacttcgta attggcgccg agcaaaccgg ccggcacccg atccacatcg
     3061 ggataatcgc cgccgcccac aaaggagtgg ccaatgctcg tttcacctcc cagaatgtcg
     3121 agaatatagt tcaaatcaga acggtggcgc acatgctcta cccaaggcga gtagcttttc
     3181 catgcccagt ccaagtcgag accatgcaca ttttctacat aaaaaaagtc gcgctgatat
     3241 cgccacgctt ccctgaatat ctgcctggct tcggccagag ggtccacctt catttgcatc
     3301 tcggaaaggt tgagtttgcc gtcgccgggc ttgggcgtac ccgatgcgtc cacgataccc
     3361 caggtgttgt ccaatgtgcc gtaaagtatt ttttttccat cagcgctgat atcgaaacgg
     3421 gtgaggccgc ccatgacctc ctctgatttg cgctcattca ttttatacct gccaacggca
     3481 aaagtggcgg ggaacgtggt gttcgggtcc acctgccatt ctccataaaa caacacgcct
     3541 tcttcccctg cgccgaggta gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3601 cccgaggccg tcaaagtcaa tgttcacctt gatcttggct gtgtcttttt tagtggtgtc
     3661 gggcttttcc tcctcgtcgc tcagcggctt cagcggggat gcttcttttt tggacaacac
     3721 agccacatag acgttctggg tggaaacccg gctgtaagaa ctcaggtcga gccagcccac
     3781 gccgagggcg aaatcggtgc tggagaggaa gtagaggtac ttgccgcttt tgtcccagac
     3841 aggcgcttta caattcgaca tgccgtcggt gaactgcatg gcttttttct gctccaaaga
     3901 atagacgaag atggtgttga actcatttcc caggcgtttg gaataggcta tccattttga
     3961 gtccggcgac caggcggggg cgatgttgcg ctcggggtgc gccgagcctt cattgtccac
     4021 cagcgtggcc ttgcccgtgg cgatatcgag gtaccagagg ttgcggtcag catcgccaaa
     4081 ccccaggtat ttcgaatcgg gcgaccagac gggcgtgtag taataggtag gattggccag
     4141 cgctattttt ttctgcactt ttccgaaggc atcagcgata aacaattgat actctccgct
     4201 ttcgtcgctg aaataagcta ttttttcgcc atccggcgag caggccgggc tgcggtcggc
     4261 aacgccggag ctcgtggtca ggttgcggat atcgcccttt tcggcgggta cgctgaagac
     4321 atcgccccgg gaagacatca acacccgttt gccgcccggc gacagcgagt attcggcaat
     4381 gccggcgccg actttttccc agtgtggccg cgcccaggga aaatctccct ccactgtgat
     4441 ggtgagtttt ttcggttcac cgcccttggc gtcaagagtg tagagcctgc cgccgttttc
     4501 aaagatgagc ttgcctgctc ccgatcggag gcttttgcaa tcaaaatccc tgaattttgt
     4561 aacttgcttc aattgcttgg tattcaaatt ataagaccaa atattcatgg caaaatcccg
     4621 gtcggaaaga aaaaatatct catctcctac ccattgtggc tcccggtcat tggcaccgtc
     4681 gaagggcagt gttatttgtt cgaaattcgc cagatcaatg atccggatgg ggttgttttg
     4741 gccgccgcgg tagttgcgaa attcattttc ccagggcata atcatctgat aggcaaagcg
     4801 tttcccatcc ggggaaaaac tgccctccca aaccctcgga atgggcaaag cctgctctaa
     4861 acctttgccg tcgggcgata ccttccagaa gcgatccggc gaggggtaag gtgcattttc
     4921 tctgccggaa gcaaaaagca catgcttgcc gtcgggcgtc cagcctttgg caatgtcggc
     4981 ctgaggatgc caggtcagac ggcggggctc gccgccttcc accggcgcca caaagacgtc
     5041 gttgttgcca tcgtactcgg cggaaaaagc gatccatttg ccgtcgggag aaaagtaggg
     5101 attgccttct gtgccggggt gggtagtcag tcggcgggga ttctttccgt ccaattctgc
     5161 cgtccataca tcgccggcat aaacgaaggc aatgtggttg gcagaaatcg tggcttgtcg
     5221 gagaagccgg gtttcctgag aaaaaccttc aatccccata acgatgagca acgcagtcgt
     5281 tgcgaacaga gaaagtcgag tgttcattgt tgcaaatttt gtcgcaagat aggcaataac
     5341 ttagttgcga cgaggcaatg gaggggaaaa tgaaatgttt tacggccaac cgaagacttt
     5401 ggcaataagg ggcgataaaa aaataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtac aatggaatgc
     5641 ttcagcgtgt tcggaagtag tgatgtctga tgttgatcct gctcctctaa ccattcatac
     5701 ccatctattt gccgggctga gaaaaggata ccttttttta cctttgcccc tttgacaact
     5761 tacaccattg taattatgga tgcctccatt ttgtaccaat tttcggaagg cgtggccacg
     5821 ctcaccctcc atcgcccgca ggtttacaac agcttcaacc gggaaatggc cctgcgcctg
     5881 atcggtcatc tcgaagcctg tgccgccgat cctgccgtgc gcgccgtact catcaccggt
     5941 gcgggcaagg ctttttgcgc cggacaagat ttgcaggaag tcgttgatcc tcaaggccct
     6001 tcgctcacca atatcctcaa agagcactac aatcccatca tcctcgccat ccgacgcatg
     6061 gagaaacccg tgatatgcgc cgtcaacggc gtggcagccg gcgcgggcgc caacatcgcg
     6121 ctggcctgcg acattactct ggcggcagca tcggcctcct tcattcaggc gttcagcaaa
     6181 atcggcctca ttcccgacag cggaggcacc tttttcctgc ccagaatcat tggttttcaa
     6241 aaagccctgg cactcgccat gaccggcgac cgcatcaacg caaccgatgc cgaactgctg
     6301 ggaatgattt acaaagtgct gccggatgaa tatctggagg aggaatccaa acggctggcc
     6361 ctcaccctgg cacagatgcc gaccgccgcg ctgggcttca ccaaacgcgc cttcaacgcc
     6421 tccatgacca acaacctgga aacccaactg caactcgaag aaaccctgca aacgactgcc
     6481 ggccaaacgg aagactttca ggaaggcgtg cgcgctttta tcgaaaaacg caaaccggtg
     6541 tttaaaggca aatgagcaaa ctactgacga ccattcgatt acatccggcc aatgagcgcg
     6601 ttgtgcaggt gctcgatcag cgccgcctgc cttttgaaga agtcatggtg gatttgcata
     6661 ccccggaaga tgcggcccac gccatccggg agatgctggt gcgcggtgct ccgctcatcg
     6721 gcgtcacggc tgcttacggc atgtatctgg cggctctgca cgaacccgct gcgagtgtgg
     6781 aatcgtatta ccaacaacaa aaatccatgc tggctgccac ccgacccacc gctgttgact
     6841 tgtttgcggc gctcgatcag gctgcgcagt ccattcttca ggccggttct cttgcggagc
     6901 ggcaacaaca ggcgctggct ttcgcccaac aatgtgccgc cgacagcata gatgcctgcc
     6961 gccgcatcgg cgaacacggc ttgcctctga tcgaggccat cgcccgacaa aaaaacggcg
     7021 cgccggtcaa tatcctcacg cattgcaacg cgggcttcct ggcctgtatc gagtacggca
     7081 cagccaccgc tccgatctat ctggcgcagg agcgcggtat tcccgttcat gtgtgggtgg
     7141 acgaaacccg gccccgaaat cagggcgccc ggctcacggc ctttgaactg ggcaggcgcg
     7201 gcgttccgca taccctcatt gccgacaacg ccggcggcca cctgatgcaa catggcttgg
     7261 tggatttggt catcaccggc accgaccgca ccacccgctg cggcgatgtg gccaataaaa
     7321 tcggcaccta tctcaaggca ttggcagctc aggataacgg ggcgccgttt tatgtggcag
     7381 cgccttcttc cagcattgac tggaacattc gcgacgggct gcgggaaatc aacatcgaac
     7441 aacgcgacga ccgcgaactg cgctacatcg aaggctgggc cgaggggcgt attcaggagg
     7501 tgctgctttg cccggaaggc agtgccggcg ccaatttcgc cttcgacgtg acgccggcgc
     7561 gcttagttac cggcatcatc accgaacgcg gagtgtgcgc tgcgagcgag gatggtttga
     7621 cgactttgtt cccggagaaa cgataaagcg gcgtgcagcg tggagccgca taccgcctac
     7681 ccctccctcg gtataaacaa aaaattactg ccctcgtcct tcaccgcaaa tacgcaaaaa
     7741 tactgccggg ggtctttata caccatcctg aaatccgcga catgatcctg cgcgaccact
     7801 tgccgggcta ccaactcctc caaggtagaa gcattcacct gccagccatt gtcggcacgc
     7861 atgagcaata ccccgatcgg cacctcctgc gtggcagcta taaaaatagg atatgacgac
     7921 acctccgcat ccagaatggt gtccgaagcc tggcccatga tgctgaaata cgggctgagt
     7981 tccgccttta tgttcaaaaa aggattgccg ttggtttcca tgtctttgtt tttatctgtt
     8041 attatcagcg ggtctccaaa actacatcga aagactcccc ttcctaaaat cagtgctgcc
     8101 aatatcggca ttgatgacgt aaggaatccc tttgtccaaa tgctgaatgg cgcgctgcac
     8161 cgcccaggca aaggcttcca gatgatccac tcgaacgcct gcagcgcaag ctcacgctcg
     8221 cacgcagccg caaacgcctc ggcaatgcgg tggtagtcgg agcggggcaa ggtcgtggcg
     8281 gtggccgcgc cgaggaaagt cacctgatcg cgggcaatct gttcccaaga gccgttattg
     8341 ccgatgatgg cgcacacttt gaagccattt ttggcaaaag tatccaattc ggccagactg
     8401 tacgctgcgc tgccgtcgcc ataaatgatc catatataat ggttgggata gcgccgcgct
     8461 gcaccgaggg caaaaccgcc gcctacgccc aaggtgccga acacgccggg atcgagccag
     8521 cccaatggct gccggggccg gagcgtatag gaggccgtag ctgcaaaatc gccgccgtcg
     8581 gctatgagga tggaattggg cggcagcatt tgctccagca ccatgaacag cgtcacagga
     8641 ttgatgccct gccctaccgt tgcgcctgcc tgccgggcta tttcctgctc gcgggcggcc
     8701 tgccaatcct gcaattgcga tttccattcg tcccattcag gccggggcgc gctaatttca
     8761 gacaggcgaa tgaggaatct gcccggatcg ccgaggatgg cccggtggcc gcgcaggttt
     8821 ttgtgcagtt cctcccgatg gcggctcact actattttta cggcctttcg gctgaggtgg
     8881 ctgccgtagt ccaaacgaaa atcgctgggt acgccggcca aaatgatgag atcggcttcg
     8941 cggagggcct ttttgcgctc gtgcaggtgc agcaacggat gctccctgcc cagcaagccc
     9001 cgcgccatgc cgctgacata caccggaata cccaatcgcg gtaacgcctc ggtcagcgcc
     9061 acgatttcct gcggcaacaa catggccccg ctgccgatga ccaacaccgg gcggcgggcc
     9121 tgctgcaatt gggctgccgt gcgctctatg gccgattctg ccgcgcgcgg aatggcgggc
     9181 gtggccggag cgccgatttt gccccctcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnncgg tcagtttttc caagagggta tttttgcggc tcgttttttg
     9301 ggcataccat tcgcgcacca gcgactcgtc gtagaggagg tccaccggta tttctacaaa
     9361 aacagggccg ggtacgccgc ttcgggcaat atgcaatgcc ttgtgcaggg cgcctgccaa
     9421 ttgcgacaaa cgccgtaccc gcagcgccca cttaacgtgc ggccgcacca atgccatctg
     9481 gtctatgtct tgcagcgcgc cgcgcccccg cagcagggta gccgtagcgc cgccgatgnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nagtcaccgc cgctacgccg ggtattccgc tgagacgggc ggctgcgtcg
     9661 gcagcaaaaa cagcacttgc ttcattgcgc acatccacaa tgcgcaaacc ggctttttca
     9721 ccctccacga ggatgggcga aatatgccct ccgcacaggg tgaaaatacg ctctactccg
     9781 tgggctttca atacctgcgc tgtgatggct cctccgttca tacttggccg aattggttga
     9841 ggcgcaaaga taatatgatt atggcgggtg gccggtacta tatacatttc aagtacattn
     9901 nnnnnnnnnn nggcactttt caatggcggt gtttgttttt ggtaaaatat tgaatgtgta
     9961 aaaaaaaaaa aaacgaaaaa atatttgtat ttaaatttca ggcactttat ctttgacata
    10021 tcgtttttcc gaacaacccg gtaaaagacc tcggattgga aaacgacaat gtattgaaca
    10081 acacgacacc tcccgagtca tcccgcgaca agggaaaagt tttagtaacg aattcaacaa
    10141 cactgtctga acaacactgc tgcggcctgc gccgtatgga aacgcctgtg ccttttgggt
    10201 accgtcttcc atattggttt tgccggagag gggattgctt tgccatgccg caannnnnnn
    10261 nnnctggtat gcagcgcagc caaaagcgtg ttgaattcga ctttaatcga accccggccg
    10321 cgcgcagcgc gggatgctgt ggcggctggg tttttgaaaa gtcgcgaatg ggaatgaatc
    10381 gtcgtgaatg ctgtatcata caggagcaga caagcccggc gcgggctgca tatatgtgag
    10441 attgtacaac acaccgcgca cgagacgagc ctgctccgca ccatgatggc cggtatatcc
    10501 ggcggcggac aaagcacccg aaccggctcc gcctgtaatg gccaagtagg ggacaaacct
    10561 tttcaacatt ctatttttat gaaaaaaatt ctggttattt taatgctgct ttcatctgcg
    10621 ttgggactcc gggcgcaggg cagtatttcc gatccgtttt tcaatcacgg gcaagcccgg
    10681 tcgctaaact cacggattat tgcgccgctt cagcctgtgc ctggctttgt aaatcaggcc
    10741 attgccggta tgcgcgtttc caacaacaga aatctggtgc gcttggctca tctcaaagac
    10801 gattatggct atgccgtttg ggatcaggcc aaaaccttca cagcagccgg taggcagacc
    10861 gccctgatac caatggttcg cccggaagac gatgccataa cgggtatttt ggtagccgta
    10921 aaggacggtg ctacgttcag gtacggcgtg atggataccg gaaccccgtt tccgtacgcc
    10981 tctgctgcga cgggtacacc gggcatgccc ggtatgcgtg atgtgcttgc cttgtcagta
    11041 ctcttcaatc gaacgctgtt tggcgaggta gattgcgatt tggtgcaggc gctcagggcg
    11101 ctgnnnnnnn nnnnnnnnnn nnnnnnnngg gggcagacgg caggcagccc caaatcctgc
    11161 tatgtccaga caacttatgc cgggcagaac tgtgaccata tttatggcac ttacccggat
    11221 ggcacgatgc aatacctcta ctcggactgc tatgatatct acaactattc gttcatttgc
    11281 gataccgaca gcgcagataa tgatgacggc ggcagtggcg gcggctccgg tggaagtggt
    11341 ggaggtaatg gttcgagtgg gccgggggaa ggtgataatg agtctttttg tgaaagctta
    11401 gatgtgcata aatgtacgac tacacgggta ctctcaactg taccgtcagg tattcatggc
    11461 tcttgtagta tagtctgtag attgtccggt tgtggatttc aaagcaacgt atttctccaa
    11521 cctaacggaa tagcaggctg tggatttagt attggtactg gaagtggtca tggtattagc
    11581 atttctctcg attttaccgt gtcaatacag aatttataca ccaattcgta cataaactct
    11641 gtttctacac tagatagtga ttgtcgagtt tcctcgattt gtggtgattt tttttgctca
    11701 tacgacgtag ttatttcttg gatcggaata ccgtatatct gggaaagaaa tagcgcggaa
    11761 ctaagcaaca tttgttacac ttgcagaaat tgtcagtaat ctcaaaaaaa taaaaaatca
    11821 tgaaaaatca atcctttttt atcttcgcat tgctcgcttt catcctcgtc agtgcctgca
    11881 aaacatctga aaacaacagg gagagcgtgc tggatgccaa acgcatggaa gtggaagctc
    11941 tggtcgcgaa gtattcttct gaaaatcttg ccggccctgt caactggcag caattcaatg
    12001 ccgctctcaa agatgaggcc gtgcttgagc aaatgaatac cgctcaatta gaagcctgtc
    12061 tgaaaagtat gactgcctac tctaataata ttattcttga aaatcgaaaa aacggatgtg
    12121 atcaactcca agaacaggtc aagagttaaa ggagtttttg gaagccaata aagatttgcg
    12181 agcctcctgc gattctttaa acaaagcgta ttcttcaggt tttcaaaagg cattagaacc
    12241 ttgtaaaggc gtcttgggag caagcgttga aagaaataaa tagaggcaat tatctacggc
    12301 agccggcgtc agcccggctg ccttcgttgt tgtcgctatc cacaggcagg ctcataattg
    12361 gcgctgtaaa aaacggcttc aagcccatca aaacaaataa ctagtcagac gatgagcatt
    12421 ccatgatcat ctcattcttt ctcttgctgt tcacagcatt ggactgcaaa acgcaggaaa
    12481 agcaggaagc gataatcaac aacaagcgcg tggaaacgga ggccattctc aacaagcacc
    12541 tccagtctat ggaatcaagc gtgaccgtca attatgacca gttccgggag tccttatccc
    12601 acgatgaatt gatagcatac attgatttga accaattggg gacgaaaaga actacgacca
    12661 aaaactcacc ataaaagtgg acgccaatgc catagtgtcg gtaacaaaca ccaacaggcc
    12721 gggcgctttc acacccgacc tgcagccatt aaacttcctg tttttcccgt aacttctata
    12781 atctcaattg cgcggcgcgg ggcgttgcgg ggctgctccg ccgcgattgg cccgcatttg
    12841 ttgcagattg tccaacagtt cctcccgaaa ctcgcgctcc aaacgcggca gcatggccac
    12901 tttgcgcacc ggcagcactt ttttcatgcg ttgcaggtag tctttgcgca ggtcgaggag
    12961 ttcctgttcc atgtccaact ggttgttcag aaacttctcc gcttcggcat ccgacataga
    13021 catgaggtct ttggccggca caaacttgtc gcggattttt ttctgctcgg cttcaaactg
    13081 attgtacaag ggccagaatt gctgcgcttc ttcgggcgtc aggttcaatt tgttggtaat
    13141 gaaggccgtg cgcatggctt ttacgcgctc gccgccgcct ctttccgggc gcggttgtgc
    13201 ttgcaatgca gccaatgcaa tgagggaaag ggcgatgctg aaaatccatt ttttcataat
    13261 gtcgcggtat tgtggtgatt agaatagttt ttccaaagag tctgcctcca actccagcag
    13321 taattcctcc agcaatcggt cggcatcttc tccgctcagg ccggaggcgc cggtccagga
    13381 agtattgccg gcaatggttt gagccaacaa atccgtgtcg aaatcttcga tgttgttttc
    13441 aatgtaggcc aaagtctctt cctcggtaag cgaggctaag gaggcgccgg cgtcgttagt
    13501 agtcggcgac caaatccaaa atgcagcaac gagggctaag accactgtgg caaaccccac
    13561 cacataagac ggccnnnnnn nnnnttttga aaacctgaaa cacggcggcc catcgctccc
    13621 atgcggtcgg gcgagccggt gcaggcgcgg cagttacctc cgtttttaat ctccaaagta
    13681 catcctgctg catatcgcgg aagtaatttt ccggtgcttc aatggctgat tccgactgcc
    13741 ggatgcggct cagcaagggc gacaaaccgg cgagttcttc tcgcgcttcc ttgtttgtat
    13801 gttcgctcat ggctttattt ttcatggaaa aaatcttcaa ttttctttac agcatgatgg
    13861 taagatgctt tgagcgctcc taccgatgta cccagcgttt tcgacatttc ttcataaggc
    13921 atctcctcgt agtacctcag tgagaagacc aatttttgct tttccggcag cgcgctgatc
    13981 gctgatctca atcgctcctg aagcgcatcg gcgtctatcg gcgcatcggc ctgcatggag
    14041 gcgctcaagt gttcgtgtgc ctgcatggag tcggtcgccc tgcgtttgac gctgttcaaa
    14101 aacgtaatgg cctcattggc agctatgcga tacaaccagc tcgacaattt ggacttgccc
    14161 tcgaactgcc cgatgtgccg gtaggctttg atgaagcagt tttgcagcag gtcgttggcg
    14221 tcgtcgtggt tgccggtcat gcgcatcagt tgccgataca agggcgcctg atacttgtcc
    14281 atcagcattt tcagcccccg ctccatgccg ccgttgctgc gcatcagagc gagtatctgc
    14341 tcgtcgggag ttgaaatgga catatacgcc aagtgccgtg ttttgtgaat ttgacttcaa
    14401 agatagggaa aggtttaatg gggaggtttc gggttcaagg tttaaggttc ccgcccgaaa
    14461 cattgaaccc ggaacgcgaa acgttgaacc ccgaaattcg gaatccgaaa caatatgccg
    14521 ataaacaaat aaacattagc tttgcgcccg caatcaacaa aaggcaggac ctggttccgg
    14581 tcttctattc tgaaaccact ttaaactata gtcaatcatg ggtcaaaatc tcctgtatgt
    14641 agttccggta ctgggtgtac tgggtttatt gtacgtcgca tggcggtctg cctgggtcag
    14701 caaacaagaa gtaggcaccg agcgcatgca ccgcattgcc aaggccattt ctgagggcgc
    14761 catggccttt ctcaaggccg aataccgcgt tttggcagta tttgtagccg tagtagccgt
    14821 attgctggcg gtttctgccg atgctcagaa atcctcctgg ttggtgggcg tatcctttct
    14881 tataggcgcc atcagttctg cgctggccgg cttcatcggt atgcgcgttg ctacgaaagc
    14941 caacgtgcgc accaccaacg ccgcccgcac cagcctcggc aaagcgcttg aagtagcctt
    15001 cgccggcggc tccgtcatgg gcatgggcgt agtaggcctg ggcgttctgg gcttgagcat
    15061 attgttcatc gtttatggca acatgttcgg cacgggcacg gctgaagacg ttacgcgcgt
    15121 catcacggta cttaccggat tttcattcgg cgcttcctcc atcgccttgt ttgcgcgtgt
    15181 gggcggaggt atttatacca aagctgccga cgtaggcgcc gacctcgtag gcaaagtgga
    15241 agccggcatt cccgaggacc acccgctcaa ccccgctacc atcgccgaca acgtgggcga
    15301 caacgtgggc gacgtagccg gtatgggcgc cgacttgttt gaatcttatg tgggcagtat
    15361 tgtcggtacg atggtattgg gcgctgcgtt catcggcctc actgagtttc agggagcagc
    15421 cttgcaaatg aatggcctca acgccgtgct gctgccattg gttctggccg gcgtaggcat
    15481 tgtaacttcc atcatcggta cgttctttgt aaaagtaaaa gaaggcggca acccgcaaaa
    15541 agcgctcaat acaggcgaac tgctctccag cgcggttatg ctggcggcta cctggtttgt
    15601 agtaaaatgg atgatgccgg ctacctgggc attcaacggc acagagtatt cttctaccgg
    15661 tatcttcttc gcggtagtga ttggcttgct cgctggtttg ggcatcggta tgattaccga
    15721 gttttacacc ggcaccggca cccgcccggt taagagcatt gtaaaccagt cagttaccgg
    15781 ctctgccacc aatatcattg ccggcctcgg cgtaggtatg caatcaacgg cagtgcctat
    15841 tttgattttg gcggcgggca tcatcggcgc ttatcacttt ggcggcctat acggcattgc
    15901 cattgcggct gttggtatgc tcagcaacct cggcattcaa ttggcggtgg acgcttacgg
    15961 ccccattgcc gacaacgccg gtggtgtagc tgaaatgagc gaactgccca aagaagtgcg
    16021 cgcccgtacc gacaaactcg acgcagtagg caacaccact gccgccatcg gtaaaggttt
    16081 tgccattggc tctgctgcac tcaccgctct ggcactcttc gcagcttaca tgcaggtagc
    16141 acaggtgaat gtcattgacg tttccaaccc gcaggtaatg gccggtttgc tcatcggttc
    16201 tatgttgccg ttcctgtttt cttccatggc aatggatgct gtaggcaaag ctgctatgga
    16261 tatgattcag gaggtgcgcc gtcagttcaa cgacattcct gaactgaaaa aggccctcga
    16321 tgtaatgcgc aaaaacgacg gcaaagaaat ggacgagtgg agcgaggcag atcgcaatgt
    16381 attccatgct gccgatggca aagccgagta tggccgttgc gtggaaattt ccaccaaagc
    16441 ggctatccgc caaatgatat tgcctggttt gttggctgtg ttgtcgccgg tactcatcgg
    16501 cttcctcggc ggtaaggaaa tgctgggcgg tttgttggcc ggcgttacct ccgcaggtgt
    16561 gttgatggct attttccaat caaatgccgg cggcgcctgg gacaatgcca aaaaaatgtt
    16621 tgaaggtggc gtggacatca acggcaagac gtactacaaa ggctccgaac cgcacaaagc
    16681 agctgttgtg ggcgacaccg taggcgatcc gttcaaggat acttccggtc cctcgctcaa
    16741 catccttctc aaactgatgt ctatcgtagc attgattctg gccccgcttt tggcaggtta
    16801 atgcactgat attgaattga tttcatctgc gtgcgcggat gttgtcggca agcccggcag
    16861 catccgcgtt tttagttggg caaactcgcg gctcatagct cgcagcttat catatcaact
    16921 ccctaagcac taccaccttc aacatccgca ccccgtttct ttctatgacc accttcattt
    16981 tttttccggc ctttccataa aacttattgg tcagttcttc cagcgtatgc accgaagcag
    17041 gccaaccgtt gacagagcga acctgatcgt tcacctgtat gcccgcactc gctgccgggc
    17101 tgccgggcaa aacgtcaaac accgtaaaag tatctaagcg tttcccggat gcagctaaga
    17161 gcatgccgct tttttcgctt ttgaaaaccg cttttgctct cgaagtaggc gaggcgtaca
    17221 gtttgctatt gggatagtca aaaatcatga caaatcggtt cagaatctgg ttgcctatga
    17281 tgccgttgcg ctgttgtgtg cgcagcgtgt catctggaaa ggggaggtct tgaaaattgg
    17341 tgatgacgcc tttcatctcc atgtcggcaa agcggagata cccgatacgc cccagaaagc
    17401 cttcaatgtt tccgccaagt cccatgccca gacggctgcg tactacctgg ggcggcaaat
    17461 gcagggaggg gtctgtgttg gtataaataa ggatggccat accggcgccc gtgtccacca
    17521 aatatttata atcgttgact tgtttgccgg caatgattgt ggaggccttg atatagggtt
    17581 tattgcggat catctccacc ggtatttcaa tgtacctgtt tccaggtggt ttgaaatgga
    17641 cgggattgat aagcgagata atctgttgtt tgtaattgat gcgtaccacg aagcgtcgga
    17701 acaagtcgga cccgatgatg ccgtgtacgt ccacgccggc aaactcttta aaattcacat
    17761 aatcttcctc cagaacaaga atggttctgt tgggcgctat caggccgcct acgttgagct
    17821 tgacgccgat agccagaaat gcgtacaatt ctacactgag atccgacccc atcagcggca
    17881 gacggcgccc gtattgtact tgcagaatat cggtgaaatc ccgttgggtg agtatggtat
    17941 gctcggcgcc ggtatcgaaa ataaaacgca ggggcagcaa atcattgagg atgacattga
    18001 cgatgatgaa attgttttcg tactcgaaag ggatgtctat tctatccaca ttgcggcgaa
    18061 gaatcaggtc gggcgattgc gacagggccg tcattgagac gccggcaaaa agcccggcaa
    18121 aaaaccacag tctaagcagc atgatttgtg tttgatcgtt aacgtattat atagtaaacg
    18181 tacaagatgc aattttgcaa attcgcaata ttatttcgta ctttttacaa agaaaaaaaa
    18241 aatgcagtgg tgcattgcaa attcagaact atttcctcac ttttgccgcc gtcttagtgt
    18301 ttgaagtaca aaaagcgaca agttgcctcc caaagccatt gaaaatcaca gctctgtgaa
    18361 acacacttat ggtattccca ttgaacataa aagagtaaac aggtaatttt tttttaacca
    18421 aaaccagcct aatgtatgaa acttagagtc aactactttt ctcaagggtt gcttctggtc
    18481 gcgtttatgc tgttgggaac ttgggcgatg gcccaacgta ctatcagcgg tattgtgacg
    18541 gatgcagcaa ccaaagaacc gctcatcggc gccaacgtat tggtggtcgg aaccagcgca
    18601 ggtacgatta ccgacattga cggtacctac tctgtgtctg taccggcagg tgctaccgag
    18661 ttggaattct cctacaccgg gtatgcttcc cagaaagttg ccatcggcgc atccagcaaa
    18721 gtggacgtgt ctctctccgc aggtcgcctc ctcgacgagg tggtggtcgt aggctacggc
    18781 acggtgcgca agggcgatgc tacgggcgct gtggtggcca tcagcgacaa agacttcaac
    18841 aaaggggtca ttgcctctcc tgagcaactg cttcagggcc gcgctgccgg tgtacagatc
    18901 acagcggcca gcggcgagcc gggcgcaggc gtcaacatcc gcattcgcgg tacatcttcc
    18961 gttcgtgcag gaaacaaccc acttttcgtg gtggacggcg tgcccctgga tggccgcgac
    19021 gacagtgccg gcggtaccga cttcggtgca ggttcgtctt ctgcccgcaa cccgctcaac
    19081 ttcctcagcc cggaagacat tgaaaccatg accgtcctga aagacgcttc cgcagccgcc
    19141 atctatggct cgcgtggcgc taatggcgta gtactcatca ccactaaaaa aggtaaagtg
    19201 ggtacatcta acgccacttt ctcagctcag accagtgttt ccaatgtgct gaagcgctac
    19261 gacctgcttt ctgccgatga gtatgtggac gctgccgtga aggccggcgc caatgctcag
    19321 gcggtgaatt tcggcggcgc taccgactgg caggaagaga ttttccgcac gggcattgct
    19381 cagaactaca atttcagcta cggcggcggc aatcagaaaa cgctgtaccg cttttcattg
    19441 ggctatctcg atcaggaagg cgtggtggaa aacacagccc tccgccgctt gagcggtcgt
    19501 ttgaacgcca gccacaaaat gctgaacgac aagctcgaat tcagcgtgca gatgacggct
    19561 tcccgtctga acaacgatta cgcgcccatc accaacaatg ccggcttcga aggcagtctg
    19621 atcggcgctg cgctgcaagc caacccgact cgccgggcag taaacgcaga cggcactttc
    19681 ccgcaatcag ttgacttccg caatccgctc gcacttctgg catatattga cgaccacggt
    19741 accacctccc gcgttttggc caatatcggc gctacctggc acattgcttc cggtttgtct
    19801 tataaactga acgtaggtgt ggataatgcg gaatcagtgc gccgcacggg tatttctccg
    19861 aaactggctt tcaacgacat tcagggccag ggccgcgcca ttgtggacaa ccgctacctc
    19921 tccagccgcg tagtagagca cacactcaac ttcaaaaagg ccatggggtc cagcacaata
    19981 gacgcagtgg caggtttttc ctatcaggag ttccaaaatc agggcgactg ggtgcaagct
    20041 cgtttctttg tgacggactt gatttcattg gtggacaact tagacggcgt caatgcacaa
    20101 gacaacaaca acgcttaccg cgccgcttcc gaccgcagcg tagaagagtt gcagtcttac
    20161 ttcggtcgtg tgaactacag catcggcgat cgcttcatct ttaccggtac ggtacgcgcc
    20221 gacggctcca ccaaatttgg tccgaacaac aagtacggta tcttccccgc catcaacggt
    20281 gcatggcgcc tgagcgagga atccttcatt cctgaagcat tttatgatct gaaattgcgc
    20341 gcaggctggg gtattacggg caatcaggaa ttccccggca atgcttccaa ggctatttta
    20401 cgcgctaaca acgcgggagt tttcgacata gtacagtttc ctaatccgga catcagatgg
    20461 gaatctacaa cacagacgaa tattggcctt gattttggct ttgccggtgg ccgcctgact
    20521 ggttcgttag actacttcaa caaagtgaca aaggatttga tcttccttca aacttttgct
    20581 caacctgctc ctggtaacgc aactcaattt ttcaaccttg atggagatgt agtcaacagc
    20641 ggtgtagagc taaccttgga tgccagagtt gtagacaaaa agtctttcaa ctggcaagtg
    20701 ctcttcaatg cagctaccct gaaaaatgag gtgcgcgacc tgcccacatt tgtcaacacc
    20761 ggcgccatca acggccaagg tcttacgggt gcttatgctc aacgtgtgat cagtggctct
    20821 ccgttgtttg ccttttactt gcgtgaattc ggcggcttta ccaacgaagg tttgggtatt
    20881 tatcccaacg gcgacttggt aacctatgca ggcagcccga ttcctacttt cacatccggt
    20941 ttgaccaaca atttcacctt cggcaacctc gacctgagca ttttctttaa tggtgttttc
    21001 gggcactata tttacaacaa cacagccaac cccatcttcc tcaaaggcaa cctgcgcaac
    21061 ggtcgcaacg taagccgcga tttggccaac agcccggaaa gccccaacaa cttcggcgaa
    21121 gcttctaccc gcttcctcga aaaaggcgat ttcgtgcgtt tgcagaacct tactgtgggc
    21181 tatacgttta acacaggaaa ctggaaaggc atcagcagcc tgcgtttgta cctcacaggc
    21241 caaaacctgc tgctcttcac gggttacacc ggctttgatc ctgaggtaaa taccgacaag
    21301 tctctgaatg gcatcccatc ctttggtatt gactatacag catacccgtc ggctcgtacc
    21361 atttctttag gcttgaatgt tggctttaac taatcaaaac tatctaagaa tcatgagaat
    21421 atcaaaattt ttcattctcc ttccggtagt ggccttgttc gccatcatgc cggcctgcac
    21481 caacctcgaa gatattgttg aggatggaag gggcgaagac gtagtggaca atgttctgcc
    21541 gcttcctcct gatgccgcgg ctgctctcag gggggtttac gaccaagtta caggcttggc
    21601 cagccaagac aatacctttg ctttgttgga gcactcctcc gacgagatga tgggccccac
    21661 cagaggcacc gactggagcg acttcggggt ttggcgccag ttgcatacgc acgactggga
    21721 tgcttctcac tcgtttgtac tgagtggctg gaatcagttg aactcaggcg tttttcaggc
    21781 tacaagagcg ctcgctgccg caggctccaa tgcccgtgtt gctgccgaag cccgctttat
    21841 gcgcgcttat ttcatgtatc tcgttaccga cctttatggt tcggttccct tccgcgaagc
    21901 tactgaaggt attttgacca tacctaaagt gtttactcgt tcagaagcta ccgatttcat
    21961 catcgctgat ttgactgccg ccatgcccaa tctgcccaat tatcaggatg tgaatccggg
    22021 cgtagccaac aaagacgccg ccaatgcgct gctggctaaa gtgtatctca acaaagctgt
    22081 gtttacggct gccaacccgg caggtcctta caccttcgct gctcccgata tgcagaaagt
    22141 gattgagcac gctgatgcgg taatcaactc cggccgtttc caactcacgc cttactggga
    22201 caacttcaca gcagacaact ctacgaagtc ccgcgagatg atctttgcca tcgttaactc
    22261 gccgggcaac tctcgtggca acctccagag ccgtcaccgc atgaccacgc actacaatca
    22321 gaatccgagc ggctggaacg gcttcaccac cattgccgat ttctacaaca agtggagcgc
    22381 cagtggttcc ggcgacgtgc gcaaaagcgc tgccattccg ggtgtgaccg acgtgagcgg
    22441 cctgcgcgcg ggcttcctcg aaggccagca gaaagaccaa aacggtaaca acctcaccga
    22501 ccgcggcggc aatcccttgg ctttcaccgt ggacgtggac ttgctgtatt ccaatgagcg
    22561 ccaaggcgtt cgttgtatca agtatccgcc ggactttacc aacgtggaca acccgggcaa
    22621 cgactacatt ctgttgcgtt atgccgacgt gttgctgatg aaagccgaag cgctgttccg
    22681 tacaggtaat gccggaggcg ctcgcgacat catcaaccag attcgcacgc accgcggcgc
    22741 tacagccatt actgctacgc tcactgccga caatattctg gacgagcgcg gctacgaact
    22801 gtattgggaa ggctggcgcc ggaacgatca ggttcgcttc ggtcagttcc tcaaggcatg
    22861 gtatgccaag ccgcagtcta acgagcgcgc tgtgttgttc cccattccgc aaattgcgtt
    22921 ggacaacaac ccgaacttga agcagaatcc gggctattga tttttttcaa tcgccctgag
    22981 gcattgtttt aacttatgaa tatgggtcag ggcgagcagt catctgttcg ccctgcctgt
    23041 ttgtgatatc acccaaaacg cgcaatcatg cgtttagcca gtttttggat tgccggctnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnacgaa ctgagcctga gcggcgagtt cgatgtgttt cgctatcgga attattataa
    23281 cggcggcggg gtagccatcg gcgacatcaa caacgacggg cggccggatg tattcctcac
    23341 ctccaatcag ggcgaaaacc gcctgtttct caacaaagga aattttgcct ttgaagacat
    23401 caccaccaaa gccggcgtgg ggggtacccg tgcctggtca accggcgtag cgatggccga
    23461 cgtgaacggc gacggtttgc tcgatattta cgtctgcaat tccggcgatg tgaagggcga
    23521 cgacaaagaa aatgaactct ttatcaacaa cggcgacctc acttttacag aaaaggcccg
    23581 cgaatacggg ctggccgata agggtttttc cactcctgcc gttttttttg atttcgaccg
    23641 cgacggcgat ctggattgct atgtactcaa caactcgttc cgccccattt ctaccctcaa
    23701 cctgcgcaac ctccggccct tccgcgatga agaaggcggc gataaattgt acgaaaatca
    23761 ggacggccgc tttgtggaca tcagcgaaaa agccggaatt tatggcagcg tcatcggctt
    23821 tggtctgggc gttacagtag gcgatgtgaa tatggacggc tggccggata tctatatttc
    23881 caatgacttt tacgagcgag actatcttta cctcaacaac aaaaacggca ctttcgcgga
    23941 ggcgctcacc gaggccatgc ctcataccag caatttcagc atgggcgccg atatggctga
    24001 catcaacaac gacggcctgc ccgatatttt tgtaacggat atgctgccgc aagaccttca
    24061 gcgcatcaaa caaacgacca actttaccac ctacgaccaa taccaactca cgctgcgcaa
    24121 cggtttccac catcaggcga tgcgcaacac catgcaactc aacaacggca acggcacctt
    24181 cagcgatgtg gcgcaggtgc tgggcgttca tgctaccgac tggagttggg gcgcgctcat
    24241 tgccgattta gacaacaatg gccacaagga tttatttgtc tgcaacggca tttacaaaga
    24301 tgtgaccgat caggattacg tcaatttttt ggccaacgac gaaaacctcg ccgccgcaga
    24361 acggggcgaa aaaattgatt ttaaacgctt tgtggacctc atgccctcca ccaaagttca
    24421 caattacgct ttcgccaatc agggcggttt gcgctttgaa gaccgctcga cggaatgggg
    24481 tttcgcactg cccacccact ccaatggcgc ggcttatgga gatttggacg gcgacggcga
    24541 cttggacctc atcatcaata atgtgaacga aaaactgttg gtgtacgaca atcgcagcaa
    24601 ctccatgtca gaaaacaagt ccttgaaaat caaaatacaa ggagaaggca agaacacttt
    24661 tgcactgggg tcgaaagtat tcgtgtatgc cggcggacaa atgatgtact acgaaaatat
    24721 gcccatgcgc ggcttccagt cttcgatgga ttatgaaatg gtcattggtt tgggggctgc
    24781 cgcccaagcc gattctgtgg tcgttttcag cccgtaccgc aagcgctgga cttcgggcgg
    24841 gccggtgcag gcaggtacga atctgacgat tgatttcagc aaggctgccg gctgcaccga
    24901 tgccgatatt ccctcgccga aacctgcagc gaagccttta ttggccgtcg ccaatgcgat
    24961 gtttggcgaa agccgccccc gacacaccga gaacacctac attgatttcg accgcgacaa
    25021 actgctctat catgcgctct ctaccgaagg tccggcattg gcagtgggcg atgtggacgg
    25081 cgacggcgca gatgattttt acctcggcgg cgcgcaccgg caggccgggc agttgtggca
    25141 gtaccggggc ggcaaatatg tgcgtgttcg ggtgccgcaa tttgaagaag atcaggtatt
    25201 tgaagacata gctgccgttt ttgccgatgt ggatggcgac ggcgatttag acctgattgt
    25261 agctgccggc gggagtgagg ttttgtacga atcggccaat ttgtcttcgc gcctgtaccg
    25321 caacgagtcc gccggagggc agattcgctt tgtgcgcgac gacgcggcat tgcccaaaat
    25381 caggcaggtg atgagttgtg tggtcgcctt tgactttgac aacgacgacg acgtggacct
    25441 gtttttcggc gcccgcctcc atgccaacgg ctacgggctg ccggcctccg ggtatctgtt
    25501 ggaaaacgac ggcaaaggca aattcaccga tgtgacggtc tccaaaggcg cgcctttccg
    25561 gcagatgggc atgataaccg gcgcagcggc ggccgatttg gaccaagatg gcagacaaga
    25621 cctcgtcatt gccggcgagt ggatgccgct gacggtttgt tacaacaaaa acggcgcttt
    25681 cccagcctcg gaaacaacaa tcatatccgg cgcgggctgg tggcaaaatg tacgcattgc
    25741 cgactggaac ggcgacggaa agcttgatgt tctgggcctc aatttgggca aaaactcccg
    25801 ctttcagggc agtgaaaatg cacctttgcg tctgtatgtc aaggactttg ataaaaacgg
    25861 cgctttggaa cccattttct gtcttcagga aaaaggcaag tatttcccta tggctctgcg
    25921 ccacgacctc ggcaaggaat tgcctatggt gaagaaaaaa ttcaactcgt acaaagaata
    25981 catggacaaa gacgtgcagg cggttttcgg cagcgacttg gacgacgcgc tgatgctcga
    26041 agccggacat tttgaaacca ctttgttttt gcaaacttcc gcccgtaact ggcaacaaca
    26101 ggctttgccc gaagaggcgc aattcagcat tttttacgat gcactgcccg aagatttcaa
    26161 cggcgacggc ctgcccgaca tccttttgac gggcaatctc tcggccgtca aacccgaagt
    26221 gggcgcttac aatgcctcct acggcaccct gctcatcaac gacgggaaag gccatttcgc
    26281 ccattggcct gcctggaaat cggggctgag ctggaagggc gaggccagag ccgtccgaac
    26341 gctgcgcctg ccgggcaatc gccgggtggc cttgtgcgca ttcaacaatg ggccggtaga
    26401 ggccgtggaa ttgccgcgat gagtttctgg ctgagattag aagataactt cgacatggtg
    26461 aagttaggcg gtaacttcga agttacctcc taactttgcc cgtacaaaaa aatcgaattc
    26521 actgataatg agaccatacc aatcactttc tctcttttct cttgttgcga tcctgttgct
    26581 aagccattgc gccggcaaaa aggaagcgcc ccgttttgaa ctgctcggtt ccgacaaaac
    26641 cggcatccgt tttcaaaaca agctcgtgcc caccggcgac atgaacatct tcaattacat
    26701 gtatttctac aacggcggcg gcgtggcggt aggcgatttg aacggcgacg atttgcccga
    26761 cgtctatttc acttccaatc aggaggagaa caagctgtac ctcaacaagg gcgatttcaa
    26821 atttgaagac atcacccaaa aggcaggcgt ggccggcgat ccgggctgga ccaccggcgt
    26881 gaccatggcc gacgtgaacg gcgatgggcg gctcgatatt tatgtgtcgc aattggggca
    26941 gcacctcaaa ttcagagggc gcaaccagtt gtacatcaac atgggcaatg accccgatgg
    27001 cgtgccggtc tttgaaaacc aggcctccca atacggcctc gatctggtgg gatacgctac
    27061 gcaggctgcc tttttcgact acgatctgga cggagatttg gacatgtaca tgctcaatca
    27121 caccgtacac cgcaacggca cttttggcta catgaaggat ttgaggtaca aaaatcaccc
    27181 cactgccgcc gaccggctcc tgcgcaatga cggcggcttt tttaccgatg tgaccaaaga
    27241 aagcggcatt tacagcagtg tgatcggctt cgggctgggc atttctaccg ccgacctcaa
    27301 cctcgacggc taccccgatg tctatgtggg caacgacttc cacgaagacg actacctcta
    27361 cctcaacaac ggcgacggca ctttccgcga ggtgcttcgc gagagcatgc agcacaccag
    27421 ccgcttctcg atgggcaacg atgtgggcga catcaacaac gacggctttt ccgacatcgt
    27481 gtctttggac atgctgccta ttgaccccgt gatgctcaaa atgtcggctg ccgaagaccc
    27541 cattgacgtg tatcgcttca agctcaacta cggctacgcg caccagtttg cccgcaacac
    27601 cctccaactc aaccgcggcg ctgtgcccgg cgacgacccg ctcnnnnnnn nnnnnnnnnn
    27661 nnnnnnnnnn nnnnggcgtc cgcttcagcg aaatcggctt gcaggccggt gtggccgcta
    27721 ccgactggag ctggtcggcc ctgtttatgg acatggacaa cgacggctgg aacgacctct
    27781 tcatttccaa cggcatacag cgccgctcca acgacatgga ctacatcaac tatgtggtgg
    27841 ccgacagcat tcaggcgcaa ttggccgacg aggtcatcag cgaccgcaac ctcaaaattg
    27901 ccgatctcat gccggaaata aaactgccca atttcgctta tcgcaacaac ggcgacctca
    27961 ccttttccga cgcgtccgcc gactggggct tcgaactgcc ctccttctcg cacggcaccg
    28021 cctacgccga tttcgacaac gacggcgacc tcgatttggt catcaacaac gtgaacgacg
    28081 aagcctcgct gtatcgcaat ctcaccaaag agtctgcctg tcaggattgc aatcacctca
    28141 aaatcagctt caaaggcaat gcgcccaatc actgggggct aggcgccaaa gtcatcgtta
    28201 acaccgacaa aggcacgcaa ctgtacgaag tatcggctac caaaggtttc caatccgcca
    28261 ccgatacccg cctgaacatt ggtctgggca acgcagccaa agtgcaggag ctcaccgtgg
    28321 tgtggcccgg cggcgcattc cagaccttgc gcgagtcacc tgccaatcag accatcactc
    28381 tgaagcaggc cgacgcaacg ggtagttttg attatgccgc ttatcgcaat cggggggtat
    28441 ccggcgcgcc gttcattact gtcatggctg ctgaaaaact gggcttggat tccgtacacc
    28501 gcgagaatga ttttgtggaa tttaaccgcg aggcgctcat tccgcacgcc gtctctaccg
    28561 aaggcccggc gctggcagta ggcgatgtga acggcgacgg attggaagat gtatatcagg
    28621 gtggcgcacg cagacaggca ggcattttga atttgcaaca gccagacggc agattcctcc
    28681 catcggtaca accggctttc ctgcaagaca gcatctggga agacgtggac gctgcttttt
    28741 tcgatgcgga cggcgacggc gacctcgacc tttttgtggc ctccggcggc aacgagtaca
    28801 acctcggcga cgcccctacc ctaccccgct tgtacctcaa cgacggcaaa ggcaattttg
    28861 aacgcagcct gcaagccctg cccaaagaag tggcgctcac ggcctccagc attgccgtca
    28921 acgattttga caatgacggc gacatggacc tgttcctcgg cgcaagagcc acgccctggc
    28981 gatacggcga agtgccgcgc agtttcctgt tgcgcaacaa cggcaagggc cttt
//
LOCUS       DAORLF010000007        28850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_903_length_28850_cov_2.408647, whole genome shotgun sequence.
ACCESSION   DAORLF010000007 DAORLF010000000
VERSION     DAORLF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28850
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_903_length_28850_cov_2.408647"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>704
                     /locus_tag="PK198_01465"
     CDS             78..>704
                     /locus_tag="PK198_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF37429.1"
                     /translation="MAEQTALSSIKRFFKLLQLDRKDIGYIYVYAAFEGLIALSLPLG
                     IQAVIGLIAGGAVSASLILLVAAVTAATALSGLLRIMQITVMETVQRRIYTRSAFDIA
                     YRIPRFRTELLSQYYAPELMNRFFDTLTLQKGIPKIITDISTSALQIFFGLLLISFYH
                     PFFAFFAVGLLLFLFIIFRFTGPPGLASNLKVSKYKYETVYWLEELARS"
     assembly_gap    705..725
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <726..1835
                     /locus_tag="PK198_01470"
     CDS             <726..1835
                     /locus_tag="PK198_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF37430.1"
                     /translation="KLAGNTDFHLKKVDYIVSKYLDFRKSHFGILLYQYSAFVILKTV
                     ITAAFLLLGSVLVIENQINIGQFVAAEIVVLLTIGSVDKMISSMETIYDVLTGLEKLG
                     FVTDLPLETEEGLCFPDIDQGSGLSLRIQELSFTYPDNAKASLHNINLDIASGERVCI
                     SGYNGAGKSTLVQILSGLHSDFQGVVSYNGFPFKSLNMNSLREHIGDYCTHEGIFRGT
                     ILENITIGHEDVSLEDALWAAQKMRLDSYISLLPEGYNTMLNSEGTNVAQGIRTKIIL
                     ARAIATRPALLAMESFLRNLDQVDRAAIAEFLTDKSNPWTLITVSNDPVVAARCDKVV
                     ILKEGRVVQTGTFDDIAKSEHFEQVFKVSDMDLLT"
     gene            1848..3230
                     /locus_tag="PK198_01475"
     CDS             1848..3230
                     /locus_tag="PK198_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF37431.1"
                     /translation="MLNISENTITHNVRTERYKSFGKMSLAGSHQLFTRWLLLALGLF
                     ILFLFLPWTQNIQSNGAVTTIKPEQRPQFIPSAIAGRIEKWYVQEGQLVRKGDTIVHI
                     SEIKAEYFDPLLVERTEQQVDAKEGAIRAYSDKAGALESQIAAMRSELDFKQQQLRNK
                     IQQAQFKIAADSADFLQAMLQDSVARIQYQRSQNLFDQGLEPRSKVEDRRVKLQETAA
                     KVVSARNKLDVSRNELGIARLDLSTVQYEYNQKIAKAESERFSTLSSIYDAEAGVNKL
                     RIESANYRQRSTFYYILAPQDCYITKTMTPGIGETVKEGDAIVSIVPADYQLAVELYI
                     NPMDLPLVHSGQEVRFIFDGWPAFIFSGWPGQSFGTFSGKVIAIDNMISDNGKYRILV
                     GADNGYKPWPRELRPGSGAQGIALLNNVPLWYEIWRQFNGFPPDFYKNGKEKAEEDKV
                     KRKAPAKSLK"
     gene            complement(3295..4272)
                     /locus_tag="PK198_01480"
     CDS             complement(3295..4272)
                     /locus_tag="PK198_01480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="HRF37432.1"
                     /translation="MTSILCGGNVRALTEGLTQRRIALSNAVLLTAYLRASKDIENFP
                     SKFTQIIHHELTQTKLSPACKIFLQWYIGLTGKSIQNVLRGDKDQLNIYLTALEQAIA
                     NAVQSSQSHFGDLQMNVSVGDIHTTIGWQGILQVFTGIGAQTLAIRGSEKSMYGKLFE
                     KLILGSLLSLLGFKMIDPHTSTLSKNVFWLSERGDKRESDATLLYKPGVGVRFDIGFI
                     GPGNTEISLDKVSRFEREMQRGKKHHFMSTVIIVDRIGEGSRIVALAKNIDGEIVQMS
                     MNHWVLEIARILHKRIGFKHDLLKMTDEETIRYIQDKMSQIEINAFASL"
     gene            complement(4379..5653)
                     /locus_tag="PK198_01485"
     CDS             complement(4379..5653)
                     /locus_tag="PK198_01485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRF37433.1"
                     /translation="MSETSKIYKRKATWQNFVSLSFINFSSEEMDKKRKYRITEVKQA
                     KSVAGDFLQSIQLDKAIDFGLPEIDDRYHIWRVPLLSKSKEAIGEVVIDAVTTFVNEK
                     KTTSKEVLESRLLGRKSINGHKEKRSTNGSSDAPKISSIRNTVGLGDSEELLIEMPAE
                     SVDLVFTSPPYYNARPEYADYLSYEEYLLKIKKIIHQCHRVLNEGRFFVMNVSPVLVR
                     RANRNESSKRIAVPFDMHRLFIEEGFEFVDDIHWVKPEGAGWATGRGRRFAADRNPLQ
                     YKPVPVTEYILVYRKATDKLIDWHIRKHPHPEVVESSKIEDGYETTNLWKIHPAYHSK
                     HPAVFPMELAEKVIKYYSFKNDVVLDPFAGSGTVGKAAVKTDRRFVLFEQDAEYMNII
                     KDEIHQWLHNKAEDVNWINTGPADNKTPYFNF"
     gene            5735..6616
                     /locus_tag="PK198_01490"
     CDS             5735..6616
                     /locus_tag="PK198_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37434.1"
                     /translation="MFTTRAAILFAILCIALPGLVAQELSASLDSVHTAIGSATHLRL
                     KLADPTHQLTPRSSAEELRAMPELEILEQTPFQKTADGWEQSFRIIAFQAGEYALPVP
                     VVDWGDSLYRAVPLRWVVTAPALPADEALAPIKDIIEEPRNLSDYLLPVFIAAAAILL
                     SLLLWHLWRRRKKEPVLPPEPELPPHVQAQKLLEELQQSGLWRSDHKQFYIRLSDIVR
                     LYISRQYRLPALTETTDLILQKMKPLCADKAIFDALREILQTSDLVKFAKARPDDAAS
                     ERLLGAAFVLMSKTTAP"
     gene            6613..7560
                     /locus_tag="PK198_01495"
     CDS             6613..7560
                     /locus_tag="PK198_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF37435.1"
                     /translation="MNWLSALVWQQPLWFLLLLLIPLLWRQSRQAPAAINWPEAMARP
                     TWRTRLWGSLPWLRALALALCVTALARPQLPLENQSLSSKGIDIGLVLDVSESMLAED
                     FAPNRLEAGKRVALQFVSRRPADRFSVTLFAGEAFSPCPLTTDHNMVREIIAGIQYGI
                     LRDNTAIGDGLATSVNRLKDSPSKSKVIILLTDGDNNTGYIQPKTAAEIAQSLGIRLY
                     TIGLAGQLSFFPGAVGPAINETLLREMAEMTGGRYFRAEDGTGLERVYAEIDRLEKTE
                     TENAVVRRAADKYYVFLWVALLVIATEWLLRYGVLRVLP"
     gene            7571..8560
                     /locus_tag="PK198_01500"
     CDS             7571..8560
                     /locus_tag="PK198_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF37436.1"
                     /translation="MWRFSHTEHLFALLLPAVLGLIYALRQAAKLRLLARIGSAELLT
                     AMMQHHSPRLERRRVAAALTGISLLVLALANPQWGSKPETVTQRSLDVVLALDISLSM
                     LAEDVPPNRLERAKQFARDLAAELRSERMGVVLLAGNAYPHIPLTTDFAAVEMALQSA
                     HPGLAPSTGTALGEAIRAATKVLGDQSPAAKVLVIISDGETHDEDAEAQAKAAAKNGM
                     VLFTVGVGTQAGGFIPVNVAGRSDYKRDERGEPVRSRLEEPLLRNIAQWGEGQYHRLG
                     SAADEGVIRQIRNHIDTLEKQEAERWQFDQFRSFYQIFLAAGLLLLAWAYRKL"
     gene            complement(8600..8673)
                     /locus_tag="PK198_01505"
     tRNA            complement(8600..8673)
                     /locus_tag="PK198_01505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8637..8639),aa:Met,seq:cat)
     gene            8817..9296
                     /locus_tag="PK198_01510"
     CDS             8817..9296
                     /locus_tag="PK198_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase"
                     /protein_id="HRF37437.1"
                     /translation="MQALVYVLITIASVAGMEFVAWFAHKYVMHGFLWSWHEDHHRPH
                     HHKHGFFERNDLFFLVFAIPSAACYIIGTATPHIHWLFVGIGISIYGLIYFLIHDVYI
                     HQRFKWFRQLDSPYSRAILRAHGAHHARQTKEDGESFGLLVVAKKYFQKRSKNSPTA"
     gene            9353..9837
                     /locus_tag="PK198_01515"
                     /pseudo
     CDS             9353..9837
                     /locus_tag="PK198_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
     gene            9886..10425
                     /locus_tag="PK198_01520"
     CDS             9886..10425
                     /locus_tag="PK198_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37438.1"
                     /translation="MLQPLRQTLPEASSIDDYLEFKILPKVRPWSEDLREQKFYLGKS
                     WMEFRDDVNFHKTVLHFFQPDGEYIRSEDGEISSGKWRFLAESNKFLITEDDKDHDGE
                     LYDLAFLDDDFFILRKHGNQRKLGKRTFFLMLHEPLAKRTNWHEAMEILYDKANRSVG
                     FYVLLFVAFLLVIVIWLLL"
     gene            complement(10432..>10774)
                     /locus_tag="PK198_01525"
     CDS             complement(10432..>10774)
                     /locus_tag="PK198_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37439.1"
                     /translation="IRELKRLAAEIKTWELSLTDESALHLAIGERLYREVKMLEQPET
                     PLRTLEKLIQTVEIIQEMKLKPDLWKAQNVYYSLVKGYKNGEWVFSSAEWEQAFFRLG
                     ELLSFRPEQGR"
     assembly_gap    10775..10796
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10797..12861)
                     /locus_tag="PK198_01530"
     CDS             complement(<10797..12861)
                     /locus_tag="PK198_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRF37440.1"
                     /translation="MNTANKFICIHGHFYQPPRENAWLEVVEMQDTAAPFHDWNARIN
                     FECYAPNAVARILDTKGKIMRITNNYGRISFNFGPTLLSWLEHADPATYRAILEADEV
                     SRVRFGGHGSAMAQVHSHIIMPLANRRDKETQVRWGIADFEYRFGRKPEGMWLAETAA
                     DTETLEVLAENGILFTVLAPRQVRSVRRAGESEWRPAHEGNLDTRRPYRCVLPSGRSI
                     ALFFYNGDLSQAVAFKGILNNGKHFAANLLDALDDNIEPQLVHIATDGESYGHHHRYG
                     EMALADCLHTIEQSGMARITNYGQFLEMFPPSMEAQIHENSSWSCVHGVERWRSNCGC
                     NSGGHPGWTQAWREPLRNALNQLRDQLAAIYEKEAGPLLRDVWTARNDYISVLLQRND
                     QTVNEFIQRHAQKEPSAEQRMKLMRLMEIQRNAILMFTSCGWFFDEISGIETNQILQY
                     ADRAMYYAAQVSEATDLRTKFVKMLADAPSNVHQSGAVNYIRNIEPARVNLQRVGMHI
                     AASSLFEAYPEHIELFNYIADSEDFERLEAGIMRLAIGRTAVKSKVTYSEKHFSFAAL
                     YLGQQNIIGNISLNMSRERFNAMQERIREAFLMPDLAGVIGVMQEYFGPDKYSIDHLF
                     KDEKRNILQIIAGKSLRQIERDFRDIYTDNYQLMAGIARNDMPVPAVWRSAAQFIINH
                     DLLQFF"
     gene            13028..>13790
                     /locus_tag="PK198_01535"
     CDS             13028..>13790
                     /locus_tag="PK198_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRF37441.1"
                     /translation="MKTHFPDEPLRSHTLDMLWQAQDEKGYIANEDIARIASQLQISE
                     VELEGVASFYHFFHRKPAGKYRIYINNSILAEFKDYRDIVRAFEKATGAPLGGTDPTG
                     TFGLYDTPCIGMCDQEPSALINFYPFTNLSPAKVDQIIDSLRSGVGVEFLANAVEDNI
                     RYTPEREKTILLREFVPGKALQALTRWTPGEVLSEIKESGLAGRGGAFFPTGVKWELC
                     SKFESDCRYIVCNADEGEPGTFKDRVLLNRLPGLLI"
     assembly_gap    13791..13827
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13828..14771
                     /locus_tag="PK198_01540"
     CDS             <13828..14771
                     /locus_tag="PK198_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRF37442.1"
                     /translation="CRGIIYLRAEYRWLLPKIHAAIEMYEHLGWLGADTTAKEPFRFD
                     IRVQIGAGSYVCGEETALLESMEGKRGEPRTKTFFPVEKGYLGRPTVINNVETLCAAA
                     RIMELGHERFSSLGTPVTRGTKLISVGGDCNRPGIYELEWGMTVAELLELCDAPDPYF
                     IQVSGPSGQCISAWDSHRRFAGEDLLCGGALMIFDSTRDILQIIRNFTAFFKHESCGM
                     CTPCRAGNFIFSRKLDKMARGLGSLQDITEMQQWGQIMKRTTRCGLGMTAPNPLLYAL
                     ERFPEYFEQKLDSTRTGFNRSFDLESALEDYREAVQS"
     gene            14782..16056
                     /locus_tag="PK198_01545"
     CDS             14782..16056
                     /locus_tag="PK198_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF37443.1"
                     /translation="MEKKLVHLTIDGRPIVAEEGQNVVEVAKANGIFIPSLCYYPHID
                     PPLGSCRVCTVMANGRSAAACTTLVKEGMDVVVNTDELQDWRKAVVEMMYAEGNHFCP
                     SCEKSGDCDLQGLGYNLGVTVSRFPHLFVDHVVDYNPQRMVIEHNRCIRCRRCVEEVL
                     TDEGQRVFSFQNRGNTTEVGIDYEQEAKLTHEQAMQAMHVCPVGAILVRGKSLARPRG
                     DRKYDHDTVVSEVPLPKITALKPHLRRKKIVATTSLAGCFGCHMSMLDIDLDLVELID
                     LVEFNKSPLTDIKKFTKRADVGIIEGGCCNSDNVEVLKAFRENCDVLIAIGECSIWGG
                     LPAMRNFVPLKECLVEAYSSATNNTESKLVIPHHPDLPEILDRVYACNEVVKIDHFVP
                     GCPPDAGHIWKTVKNVLFGEKYAITHSEFKYD"
     assembly_gap    16440..16459
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16460..17720
                     /locus_tag="PK198_01550"
     CDS             <16460..17720
                     /locus_tag="PK198_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRF37444.1"
                     /translation="CGICPVSHHLAAAKAIDRLHGLEPEDLSPTAGKLRRLLHYGQIF
                     QSHALHFFYLASPDLLFGADAPVEQRNVVAVAKANQELARKGILMRKFGQEIIKAIAG
                     KRSHGICAVPGGAYKTFSEAERDYFLMGRAIPSIDTMTNWAKETVDFIKKYHEDHAAE
                     LDEFAHFPSGHLGLVTPEGDLELYNGRMRAIDAEGKITLPDIHDSLYREYFAEAVEPW
                     TYLKFPYLKNIGREHGWNRVGPLSRLNVADRIPTPLAQQEFEIFRAAGTPNHRSMYYH
                     WARLIEMLHCAEVMKDLLLDEEITGDDLLREGERQPQGIGIIEAPRGTLIHYYEADEK
                     GKVTKCNLIVSTTHNNEGMNRAVRSVAAAELDRRPEITDGMLNKVEVAIRAYDPCLSC
                     ATHALGQMPLQVQVFDHEGKLVQERVR"
     assembly_gap    17892..17909
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17910..18293)
                     /locus_tag="PK198_01555"
     CDS             complement(<17910..18293)
                     /locus_tag="PK198_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRF37445.1"
                     /translation="MSARERIKQFFQENVGRVVTTGEIAKIAGINDYQRRIRELRNEF
                     GMKIRSNVDRADLKPGEYLLESLDLDPAIERSISLQLRNFILERNGFTCTICGASAHD
                     YDDLDPTKKVRLHIDHIIPLSQGGTN"
     gene            complement(18297..18668)
                     /locus_tag="PK198_01560"
     CDS             complement(18297..18668)
                     /locus_tag="PK198_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRF37446.1"
                     /translation="MARIKNRGTKPELIVRQVLLESGIRYRYNYNGLPCKPDFIFPGR
                     KKVIFVHGCFWHRHPGCKRASTPSSNTLFWETKFNKNVARDAKNQLLLEQAGWEVLVV
                     WQCNLKDKELLQSTILSFLTN"
     gene            complement(18700..19890)
                     /locus_tag="PK198_01565"
     CDS             complement(18700..19890)
                     /locus_tag="PK198_01565"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRF37447.1"
                     /translation="MESATYIQQPLKKKVSSGISKSKQRDTSYKLIDLFAGAGGMTLG
                     FTKIFGNHFESVWANDFNKDACETYRANFGDHCHEGDILSILDDPSTVIPKADVVIGG
                     PPCQGFSLLNKHREGDPRKQMWMPFLEVVERSQAEVFVMENVPQILGSPEHEQIMETA
                     ESMGFQMAWAKLCAADYGVPQKRFRAFIVGCKFADPKKVFPPPKTHFDPSARLSPLNN
                     LFSREEYAENPIPWRTVRDAISDLPPPIGTEPSVDSTPLNIHFGRTPTETSVLRYKAI
                     PHEGMNRFDLERNAPELTPDCWKRKKSGGTDLFGRLWWDRPSFTIRTEFFKPEKGRYL
                     HPAQHRPITHREAARFQSFPDTFAFKGTKIEIARQIGNAVPPMLAAAVARSVEKLLEI
                     KRNG"
     gene            20113..21345
                     /locus_tag="PK198_01570"
     CDS             20113..21345
                     /locus_tag="PK198_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF37448.1"
                     /translation="MPKISQRGECTPLSPFRKFAPLAERAKAQGRKIYHLNIGQPDIL
                     TPPQALQAVREHPLRVLAYSSADGNLSYRQKLTGYYRSFGVELAPNQIIVTTGASEAI
                     YLGLLSCFNQGDEILSPEPFYANYNGFAHMADIEVRAIPCRIEDGFALPSAADFERCL
                     TPKTKGIFLCNPNNPTGCVYPEATLRELAEVVKRHDLYLFVDEVYREFCYDGQDFFSA
                     LRLDDIREHVVVVDSISKRYSACGARIGALITYNERVLEAVNRYAMLRLSPPGFGQIL
                     AEAALDVDAGYLDEVKIEYDLRRQTVFRRLQQMPGVTSYLPGGAFYCFARFPIDDADK
                     FCRWLLEDFEYQGATVMLSPGAGFYATPGAGLNEVRMAYVICRDDLEAAMDCLERALM
                     EYPGRVETALPILEEAIL"
     gene            21342..22034
                     /locus_tag="PK198_01575"
     CDS             21342..22034
                     /locus_tag="PK198_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF37449.1"
                     /translation="MSRQPAEEQRLAESWDINAAAWTQSVREGLIRSRQLVTDRAITE
                     AVLRRRPTRVLDAGCGEGWLARKLTSQGIAVTGFDGSESLIADAQQHGGGPFFTLSYD
                     DFCAQAHVLGEPFDVVVFNFALLSEELSPILRAASQTLAPGGCILIQTLHPVEASQGQ
                     GYENGWREEHFSGMGEGYKAAMPWFFRTLSSWLDELRRAGLDCVALEEPLHPETGRPA
                     SLILTAVPRPQI"
     gene            22041..22775
                     /locus_tag="PK198_01580"
     CDS             22041..22775
                     /locus_tag="PK198_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF37450.1"
                     /translation="MATEHTTIKIQDIEVPVRIHREMRMGARASLRQDAAVLRIPRYL
                     PEWEEQRLKKWFEDWLRLQMESKPHLLERLQSKVFSDGDIVTVGARQYHLAIEERDTK
                     GHSARLRGGVIHIQLSRSGSESNQKEAIATLLRRIVAQDQLPALTRRVHELNALYFKQ
                     NIKSVRLKYNRSNWGSCSRSGNINLSSRLLFAPTEVQDYVIIHELAHLLEFNHSHRFW
                     DIVAAADPQYKTHVEWLKKHGHDCDF"
     gene            complement(22784..24022)
                     /locus_tag="PK198_01585"
     CDS             complement(22784..24022)
                     /locus_tag="PK198_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF37451.1"
                     /translation="MNIPNEVGAAGPQNIAIQDYDYPLPDERIAKFPLPERDASKLLV
                     YRKGSISETVFRNLAEELPQGAVLLFNDSRVIHARLRFTTPAGRMVKIFCLDPASPAE
                     YQMNLSSREPVIWHCLVGNNRHWKGGLLQSEVVTPAGPVTLSAERLERREGAFEVRFS
                     WQAPTLLSFGEVLAAAGALPLPPYLHRDSEPDDEDRYQTIYARQEGSVAAPTAGLHFT
                     ERVFEGLDKRGVERLFVTLHVGAGTFKPVKSETIGEHHMHEETVIVNRDTIARIAEVL
                     EQGRPVIPVGTTSMRVLESLYWHGLTAAEATTLHIRQWTPYEPQENEAPAVEVLRQLL
                     RRLDKAGQSFVEGQTQVLIAPGYRFRIAGGLITNFHQPRSTLLLLIAAWAGQDWRRMY
                     DYALANDFRFLSYGDGSLLL"
     gene            24194..>24832
                     /locus_tag="PK198_01590"
     CDS             24194..>24832
                     /locus_tag="PK198_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37452.1"
                     /translation="MKLSHILLALLLPAAMIRTAAQPAEGGLPLGRDIEFASDKSIFT
                     VAAPDVERLRQEDLSRSGFRFAAPLAVSISPENAGEWTTLPNGDRIWRIRLHAPGAKA
                     VSALYDNFYLPPGARLFMYSPDGTQILGAYTDRNNSKTGRFMTGFISGETAVVEYFEP
                     VYRYGQGRFHIFRLDYAYHSDPNQIEKSALESNNFGFGASWDCHPNATCPPAD"
     assembly_gap    24833..24847
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24848..26107
                     /locus_tag="PK198_01595"
     CDS             <24848..26107
                     /locus_tag="PK198_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF37453.1"
                     /translation="RSACRIIIVVTEGSGYCTGTLVNNVRNNGVPYVISGFHCMDGYT
                     PLWDLWRFDFRYQSSTCTNPATEPGFQSMLGCDNRAGRRENDFLLLELFQNIPASYQA
                     YFAGWDRSTNVPARSTYFHHPLGDIKKISVDTQAAVIQNTPIQWNNTVTTPTQHHFRV
                     RPDIGSFQVGSSGAGLFNHNKRFVGQLHGGFSTCDSTVAYFGRMALAWNGGGSPITRL
                     RDWLDPDDTDSLRIAGIENPAAGSVITLTGTVRTESGMPIPGVRVWLEGMLADTATTG
                     ASGEFTFPLLDAGMDYTLGFLKDTHHSNGVSTQDVIRIQRQILSIENLNSAYKQFAAD
                     VNQSGNVSTLDVILLRRIILTIMDKFPDVRSWQFFPANHTFADPLHPFGELTPNRVPL
                     GMLTGSISINITGVKSGDVNESADPSN"
     gene            complement(26116..>27099)
                     /locus_tag="PK198_01600"
     CDS             complement(26116..>27099)
                     /locus_tag="PK198_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37454.1"
                     /translation="SAENYDATIAATYAMLQDKTLSMELQAETVRSDSVTFALKLTNR
                     AGHKFPSGYPSRRALVSFVALTEQGDTLFRSGMLQPDYEVEGHDPVVEPHYQMITRPD
                     QAQIYEMVMGDAAGQLTTVLLRGYEPLKDNRLPPLGFRTTHSAYDTTRIFGNALNDPD
                     FNKVNGVEGTGSDIVRYRISVNGYQGNIRAYATVYYQSLPPKWMEEIFADSTPEIETF
                     RTMFNNADRSPVAVSSAQTGNVYVDGVTAARESALQQAIRVFPNPAPDGKCQVLADGL
                     NVLGIRVFDAKGSWLKSLPAGATEVSLPGRGVFLLEIRTDKGTLTQRVLAR"
     assembly_gap    27100..27114
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27115..28059)
                     /locus_tag="PK198_01605"
     CDS             complement(<27115..28059)
                     /locus_tag="PK198_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37455.1"
                     /translation="MPGLSKHFIFGGLLLGLIYSAAQWTSYGPPRFHTPQEIQAFHAN
                     SMLEVVDSNVIFPTSKACVGCHGRDENGYASVDLAGNDVNVVDDWSATMMANSAKDPF
                     WRAKVSHEILIHPMHSAAIQDKCTSCHAPNGRYTAHYRGHGPYSIADMLADSIGMDGV
                     SCSTCHKISQQDFGLTFSGQINFDTARVIYGPYYEPFAAPMEDFVGFRPLHGQHVPEA
                     GLCAPCHTLITHPVNAQGEFLDIAFVEQATYHEWLNSTYGLQNVTCQDCHFSRIKDPV
                     VISSNYLFLDGRVPFGIHEMAGANTMMLRLMKQNRQALG"
     gene            complement(28237..>28850)
                     /locus_tag="PK198_01610"
     CDS             complement(28237..>28850)
                     /locus_tag="PK198_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRF37456.1"
                     /translation="QETDSPVAGIFEWPSREKPWGTGHAVLMAKDVIQEPFAVVNADD
                     YYGADAFYKMAEFLTRDCHASHLAMVGYRIKNTLSEHGTVSRGVCELNDKGQLVGVTE
                     RHKIERTDTGIVFADEHGNPSPLDDNAIVSMNLWGLPVQIFTEIERQFLDFFEASKNN
                     PKAEFYIPTVVNRLLSEGRAAVSVLPNEEQWYGVTYREDKDIA"
BASE COUNT         6707 a         7738 c         7341 g         6916 t
ORIGIN      
        1 ctaagaatga ccaagcttgt agcttgcggc ctacagcttg tggcatttca gaaaaacaaa
       61 aaatgatgta aataaaaatg gcagaacaaa cagcgctcag ttccatcaaa cgttttttca
      121 agctgcttca attagatcgc aaagacatcg gctacattta tgtctatgct gcatttgagg
      181 ggcttattgc actcagtctt ccattgggca ttcaggcggt catcgggttg atcgccggcg
      241 gcgccgtatc ggcctcgctc atcttgttgg tggccgcagt gacggcagct acggccctgt
      301 cgggtttgct tcgtattatg caaatcacgg taatggaaac cgtgcagcgc cgcatttaca
      361 ctcgttctgc attcgacatc gcttatcgga taccccgatt tcgcaccgag ttgctttcgc
      421 agtactatgc gccggagttg atgaaccgct ttttcgacac cctcaccctc cagaaaggca
      481 ttcccaaaat cattaccgat atttctacct cggccctgca aatattcttc gggctgctgc
      541 tcatttcgtt ttaccacccg ttttttgctt ttttcgcagt gggcttgctc ttgtttttgt
      601 tcatcatttt ccggtttacc ggcccgcccg gattggcatc caacctcaag gtgtcgaagt
      661 acaagtacga aacagtgtat tggctggagg aactggctcg ctccnnnnnn nnnnnnnnnn
      721 nnnnnaaatt agccggcaat acagatttcc acctcaaaaa agtggattac atcgtgagca
      781 aatacttgga ttttcgcaaa tcgcatttcg gaattctgct gtaccaatac agcgctttcg
      841 ttatactcaa aaccgtgatc acggcggcat ttttattact tggttctgtg ttggtcattg
      901 aaaatcagat caatataggg cagtttgtgg ctgctgaaat tgtggtattg ctcaccatcg
      961 gctctgtgga caagatgatt tccagcatgg aaacgattta tgacgtgctc actggtctgg
     1021 aaaaattagg ctttgtaacc gacctgccct tagaaacaga agagggcctt tgttttcccg
     1081 atatagatca aggttcgggc ttgagtttgc gcatacaaga actcagtttt acctaccctg
     1141 acaacgccaa agcctcgttg cacaacatca atttagacat cgcgtcgggc gagcgggtct
     1201 gcatctccgg ctacaacggc gccggaaagt ccaccctggt gcaaatactc tccggcctgc
     1261 acagcgattt tcaaggcgtg gtttcttaca atggattccc attcaaaagc ctgaatatga
     1321 acagcctgcg ggaacacatc ggcgactatt gcacgcacga gggcattttc aggggtacta
     1381 ttttggaaaa catcaccatc gggcacgaag acgtgagtct cgaagatgcg ctctgggcag
     1441 ctcagaaaat gcgtttggac agctacatca gcctgctccc ggagggctac aatactatgc
     1501 tcaactcgga gggcaccaat gtagctcaag gcattcgtac caaaatcatt ctggcacgcg
     1561 ccattgctac ccgcccggcc ctgctcgcca tggagagttt tttgcgcaat ctggatcagg
     1621 tggaccgggc cgctatcgcc gaatttctca ccgacaaatc gaacccctgg accttgataa
     1681 ccgtgtcaaa cgaccccgtg gtggccgccc gctgcgataa agtggtcatc ctcaaggaag
     1741 ggcgcgttgt gcaaaccgga acgtttgacg atattgccaa aagcgaacat tttgagcagg
     1801 tgttcaaagt gtctgatatg gatttactga cctgataacc tcgcgccatg ttgaatattt
     1861 cagaaaatac catcacgcac aacgttcgca ccgagcgata caagtcattc ggaaaaatga
     1921 gtttggcagg cagccatcag ttgttcacgc gctggttgct gctggctttg gggttgttca
     1981 ttctgttcct gtttttgccc tggacacaaa acatacagtc gaacggggcc gtcactacca
     2041 ttaaacccga acaaaggccg cagttcatcc cctctgccat agccggccgc atcgagaaat
     2101 ggtatgtgca ggaaggacag ctcgtgcgca aaggcgatac catcgtccac atctccgaaa
     2161 taaaggctga gtattttgat ccgctgttgg tggaacgcac cgagcagcag gtggacgcca
     2221 aagagggcgc tattcgggcc tactccgaca aagcgggcgc gttggagagt caaattgcag
     2281 ccatgcgttc ggaattggat tttaaacaac aacaactgcg gaataaaatc cagcaggctc
     2341 aattcaaaat tgccgccgac agcgccgatt tcctgcaggc catgctgcaa gactcagtgg
     2401 cccgcataca gtatcagcgc tcgcaaaacc tgtttgatca aggactcgaa ccccgctcca
     2461 aagtggaaga ccgccgggtg aaattgcagg aaactgccgc caaggtggta tcggcccgca
     2521 acaaattgga cgtgagtcgc aacgaactcg gcatcgcccg gctcgatctc agtacggtgc
     2581 agtacgagta caatcagaaa atagcgaagg cagaatcgga gcgattcagc accctgtcgt
     2641 ccatttacga cgccgaggcc ggggtcaata agctgcgcat agaatcggcc aactaccgcc
     2701 agcgcagcac tttttattac attctggcgc cacaggactg ctacattaca aaaaccatga
     2761 cgcccggcat cggcgaaacg gtgaaagaag gcgacgccat tgtgagcatt gtacctgccg
     2821 attatcaatt ggccgtagaa ttgtatatca atccgatgga cctgccgctc gtccattccg
     2881 ggcaggaagt gcgttttatt ttcgatggct ggccggcatt tattttcagc ggctggccgg
     2941 ggcagtcgtt cgggactttc agcggcaagg tcattgccat agacaatatg atcagcgaca
     3001 acggcaaata ccgcattctg gtaggcgccg acaatggcta caagccctgg ccgcgagagc
     3061 tgaggcccgg ctcgggcgct cagggcatag cattgctcaa caacgtgccg ctgtggtatg
     3121 aaatatggcg gcagttcaat ggtttcccgc ccgattttta caaaaatgga aaagaaaaag
     3181 ccgaggagga caaggtgaag cgcaaggcgc cggcgaaatc gttgaagtga ggaaaaaaaa
     3241 cacaccattc atcatggcct tagagcgcag ccgtaaatgc ggttaaattg aaagtcaaag
     3301 gcttgcaaaa gcattgattt ctatttggct cattttgtct tgtatatagc gtatggtttc
     3361 ttcatccgtc attttgagaa gatcgtgttt gaaccctatt cttttgtgga ggatgcgggc
     3421 aatctccaat acccaatgat tcatagacat ctgaacgatt tctccatcta tattttttgc
     3481 aagcgccaca atgcggctcc cctcacctat cctgtccaca ataatgactg ttgacatgaa
     3541 gtgatgcttt ttgccccgct gcatctcccg ttcaaatctc gatactttat ccagcgatat
     3601 ctccgtattt cccggcccga taaaaccgat gtcaaatctt acgcccacgc caggtttgta
     3661 aagcagcgtg gcatcgcttt ctcttttatc gccacgttca gaaagccaaa agacattttt
     3721 ggacagggtt gatgtgtgtg gatcaatcat cttgaatccc aataaggata ataacgaacc
     3781 caatatcaat ttttcaaaca gcttcccgta catggatttc tctgaacctc gaatggccaa
     3841 tgtttgagcg cctatgcctg taaaaacctg aagaatcccc tgccagccaa tggttgtgtg
     3901 aatgtcgccc acagatacat tcatttgtaa atctccaaag tgggattgtg aagattggac
     3961 agcgtttgcg atggcctgtt cgagtgctgt caggtagata ttgagttgat ctttatcccc
     4021 gcgcaataca ttctgaatgc ttttgcccgt caggccaata taccattgca gaaagatttt
     4081 acaagccggc gacagcttgg tctgggtaag ttcgtgatga ataatttggg taaattttga
     4141 tgggaagttt tctatatcct tcgatgcgcg caaataggcc gtcaacaaaa cagcattgga
     4201 taaggcgata cgtcgttggg tcaagccctc cgtcaaagcg cgtacattgc cgccacacag
     4261 tatggaggtc acaacatctg attacatcgc ttcctactct ttcgagcaat tcattgccac
     4321 tgaatttagc catgcccaag ccgagatcag ggatgtattt tgaaaaggat tctttcattt
     4381 agaagttgaa atatggcgtt ttgttatctg ccggaccggt atttatccaa tttacatctt
     4441 cggctttatt gtgcagccat tggtgtattt catctttaat gatattcatg tattccgcat
     4501 cttgctcgaa gagtacaaat cgccggtctg ttttgacggc ggcttttcct acggttccac
     4561 ttccggcaaa agggtcgagt actacatcat ttttaaagga atagtatttg attacttttt
     4621 cggccagttc catagggaat acggcaggat gcttggagtg ataagccggg tgtatcttcc
     4681 acaggttcgt agtctcgtat ccatcttcaa ttttggatga ttctacgact tccggatgtg
     4741 gatgctttct gatatgccag tctattaatt tgtctgttgc ttttcggtaa accaaaatgt
     4801 actcggttac aggaacaggt ttgtattgaa gcggatttct gtcggcggca aatcttcgcc
     4861 cccttcctgt ggcccagccg gcgccttcag gttttaccca atgaatatcg tcaacgaact
     4921 cgaaaccctc ttctatgaac aatcggtgca tatcaaaagg tacggcaatt ctcttggatg
     4981 attcatttcg attggctctt cgaaccagta caggagatac gttcattaca aaaaagcggc
     5041 cttcgtttaa tactctgtgg cattgatgga tgattttttt gatcttcaaa agatactcct
     5101 cgtatgacag gtaatcagca tactcaggcc gcgcattgta ataaggagga gaagtaaata
     5161 ccaaatcaac cgattctgcg ggcatttcaa tgaggagttc ttcagaatct ccaagcccga
     5221 cggtgtttcg tatggatgat attttggggg catcgctcga gccattcgtg ctgcgtttct
     5281 ctttgtggcc atttatactt tttcgaccca ataatctgga ctcaagtacc tccttgcttg
     5341 ttgttttctt ttcattgacg aaagttgtaa cagcatcaat aacaacctcg ccaattgcct
     5401 ctttagattt gctaagtaac ggaactctcc aaatgtggta cctgtcgtct atttccggca
     5461 aaccaaagtc tattgccttg tctaattgga tagattgcag gaaatctccc gcaacagatt
     5521 ttgcctgctt tacttcggta attctatatt ttcttttctt gtccatttct tctgaagaga
     5581 agtttatgaa agacaaagat acaaagttct gccaagtagc cttgcgcttg tagatttttg
     5641 aagtctcaga cattgggtga accggcgtat gtcggattca ttcacaaaag ggtagtttca
     5701 tactttttct cccatttgct gctatttttg cagcatgttc actactcgcg ctgccatcct
     5761 gtttgctata ctttgtattg ccctgcccgg ccttgtggcc caggagttgt cggcgtcatt
     5821 ggacagcgtg cacacagcaa taggcagcgc tacacatctg aggctcaagc tcgccgaccc
     5881 aactcatcag ttgacgcccc gctcctctgc cgaagaactg cgcgccatgc cggaactgga
     5941 aatattggaa caaacgccgt ttcagaaaac tgccgatggc tgggagcaat cgttccgaat
     6001 catcgctttt caggccggcg aatacgccct ccctgtgccg gtagtggact ggggcgacag
     6061 cctgtaccga gcggtcccgc tccgatgggt cgtcaccgct ccggcattgc ccgccgacga
     6121 ggcacttgct cccatcaaag acatcatcga agaaccccga aatctcagcg attacctgct
     6181 gcctgtattc atagcagcgg cggccatatt actcagcctt ctactgtggc acttgtggcg
     6241 acgcagaaaa aaagagccgg tactgcctcc cgaaccggaa ttgccaccgc atgtacaagc
     6301 tcaaaagctg ctggaagaat tgcagcaaag cggcctctgg cgcagcgatc acaaacagtt
     6361 ttacattcgc ctcagcgaca ttgtgcggct ctacatttcg cggcaatacc gccttcccgc
     6421 cctcacggaa acgaccgatc ttatcttgca gaaaatgaag ccattgtgcg ccgataaagc
     6481 aatcttcgat gcgctgagag aaatactgca aacctccgac cttgtaaaat ttgccaaagc
     6541 ccgacccgac gacgctgcga gcgaacggct gctcggagcg gcatttgtgc tgatgtcaaa
     6601 aaccacagcg ccatgaactg gctctctgcg ctcgtgtggc aacaaccgct ctggttttta
     6661 ttgctactgc tgattccgct gctttggcga cagtcccgac aagccccggc agccatcaac
     6721 tggcccgaag caatggcccg gcctacctgg cgcacccggc tttggggcag cttgccctgg
     6781 ctgcgggctt tggctttggc gctgtgtgtg acagccttgg cccggcctca attgccactt
     6841 gaaaatcaaa gccttagttc caaaggaatt gacatcggtc tggtactgga tgtatcggaa
     6901 agtatgctgg ctgaggactt cgcgcccaat cgcctcgaag ctggcaaacg cgtggcgctc
     6961 cagtttgtga gccgccgccc tgccgaccga ttcagcgtta ccctgtttgc gggagaagcc
     7021 ttttcgccct gcccgctgac gacagatcac aacatggtaa gggagatcat tgccggcatt
     7081 cagtacggca ttttgcgcga taatacagcc atcggcgacg ggttggccac ctctgtcaac
     7141 cggctcaaag actcgccctc caaaagcaag gtcatcatcc tgctcaccga cggcgacaac
     7201 aacacaggct acatacagcc caaaacagca gcggaaattg cgcaatcgct gggcatccgg
     7261 ctctacacca tcgggctggc cgggcagttg tcgttttttc ccggcgccgt tggccctgcc
     7321 atcaatgaaa ccctgctgcg cgaaatggcc gaaatgactg gcgggcgcta tttccgggca
     7381 gaagacggca ccggtctgga gcgcgtatat gccgaaattg accgccttga aaaaaccgaa
     7441 acagaaaatg ccgtggtgcg ccgggcagcc gacaagtatt atgtgttcct ttgggtagca
     7501 ttgttggtta tcgcgacaga gtggttgctg cggtacgggg tactgagggt gttgccttaa
     7561 aaaattgatt atgtggagat tctcacatac cgaacacctt ttcgcgctgc tgttgccggc
     7621 agtactgggc ttgatttatg cgctccgaca ggcagcaaaa ctacgtctgc tggcgcgcat
     7681 cggttcagcg gaactactga ctgccatgat gcaacatcac tcgccccgtt tggaaagacg
     7741 acgggtagct gccgcgctga caggcatatc gctgttggtg ctggcattgg ccaatccgca
     7801 gtggggcagc aagccggaaa ccgtaacgca acgcagctta gacgtcgtac tcgcactcga
     7861 tatttcgctc agtatgctgg ccgaggatgt gccgcccaac cgattggaac gagccaaaca
     7921 atttgcccgc gatctggccg ccgaactgcg cagcgaacgc atgggcgtgg tattattggc
     7981 cggaaatgcc tatccgcaca taccgctcac caccgatttt gccgccgtag aaatggccct
     8041 gcaaagcgcg catccgggct tggcgccgag tacgggtacc gccctcggtg aagccattcg
     8101 tgcagcgacg aaggtgctcg gcgaccaaag ccccgccgct aaagtgttgg ttatcatcag
     8161 cgacggagaa actcacgacg aagacgccga ggcgcaggcc aaagctgccg ctaaaaacgg
     8221 aatggtacta tttacagtgg gcgtaggcac tcaggccggc gggttcatcc cggtcaacgt
     8281 ggccggtcgc agcgattata agcgggacga acgcggcgaa cccgtgcgca gccgcttgga
     8341 ggaaccactc ctgcgcaaca ttgcccaatg gggcgagggg caatatcacc gcctcggcag
     8401 cgcggccgac gagggcgtta tccggcagat tcgcaaccat attgatacct tggaaaagca
     8461 ggaagccgag cgctggcagt tcgatcagtt caggagtttt taccagattt ttttggcggc
     8521 gggcttgctg ttgctggcct gggcgtatcg gaagctgtaa atggaaacgc cccgcaggag
     8581 atgctgcgag gcgttggtgt agcacggggg agacttgaac tcccgacctc atgattatga
     8641 atcatgcgct ctaaccggct gagctaccgt gccgtgcttt caaaagcgag cgcaaagata
     8701 aggtgattgt cggtaaaata gcaagtgttt gatagagata aattttcagc atcttttctg
     8761 aacgaattgc cggtatcttc gttccactct cattcgttca gtaatctcca aactatatgc
     8821 aagcgcttgt ttatgttctg attaccatag cctcggtggc aggtatggag tttgtggcct
     8881 ggtttgccca caaatatgtc atgcacggtt ttttgtggag ctggcatgaa gaccatcacc
     8941 ggccacacca ccataaacac ggctttttcg agcgcaacga cttgtttttc ttggtctttg
     9001 ccattccaag cgcagcctgt tacatcattg gtacggccac gccacacatc cattggctgt
     9061 ttgtaggcat cggcatttcc atttacggcc tcatttattt tctcatccac gatgtgtata
     9121 ttcatcagcg gttcaagtgg ttccggcaat tggacagccc gtacagccgg gccatcctgc
     9181 gggcgcacgg cgcacaccac gcacgccaga cgaaagaaga cggcgaatcg tttgggttgc
     9241 tggtggtagc caaaaaatat ttccaaaaac gcagtaaaaa cagccccaca gcttgatttt
     9301 tcaacaggca gtagtatttt tgggcattgt ttaacacaaa aaccttgttc tcatgtctga
     9361 aaacactgat ctgatcctcg atgattttga ggccccccaa tcgtttcgcc ccgaatatgc
     9421 cggcttttgg atacgcgtgg cggcctatct gattgactcc atcgtaattc aggtagccac
     9481 ctctattctg atgtttatct tcatggggtc attgtttttg aatcccgaat ccatggaaaa
     9541 tccagatcaa gtgttgggcc gggtcttacc tctttatgta atactcattg ttggttcctg
     9601 gctgccttaa tggaatcctc cgctcagcag ggtacgctgg gcaaaatggc cgtcggcatc
     9661 aaagtgacag acatggacgg cgccagaatt tcttttggta acgcaaccgg ccgctttttt
     9721 ggcaaaatga tttccggcct catcttgggc gtaggctacc tcatggtcgc attcaccgac
     9781 aagaagcagg gcctacacga tatgatggca ggtacactgg tggttcaaaa aaggtaatca
     9841 tctgtttttt aagtctttga tgtggaaata aatctgttcg ataaaatgct gcaaccgctc
     9901 cggcaaaccc tgccggaagc gagcagtatt gacgattatt tagagttcaa gatactgcct
     9961 aaagtgcgcc cctggagcga agacctccgc gagcagaaat tttatctggg caagtcctgg
    10021 atggagttcc gagacgatgt gaacttccac aaaacagtgc tgcatttttt tcaacccgat
    10081 ggagagtaca tccggtcgga agatggcgaa atctccagcg gcaaatggcg gtttctggca
    10141 gaatcgaaca aattcctcat cactgaagat gacaaagacc acgatggcga actgtatgac
    10201 ctggcttttt tagacgacga tttttttatc ctgcgcaagc acggcaatca gcgcaaattg
    10261 ggcaagcgta cctttttcct catgctccac gagcctttgg ccaaaagaac caactggcac
    10321 gaggccatgg aaattttgta cgacaaagcc aatcgaagcg taggatttta tgtccttttg
    10381 ttcgtcgctt ttctgctggt gatcgttatc tggctgctgt tgtgaaccgg gctatcggcc
    10441 ctgctccggg cgaaaactca acaactcgcc cagcctgaaa aacgcctgct cccactcggc
    10501 gctgctgaac acccactcgc cgtttttata ccctttgacc agcgaataat agacgttttg
    10561 cgccttccat aagtccggtt tcaatttcat ttcctgaatg atctccacgg tctgtatgag
    10621 tttttcgagg gtgcgcagag gcgtttcagg ctgttccagc attttaacct cgcgatacag
    10681 gcgctcgcca atggccaagt gcaaggccga ctcatcggtg agggaaagct cccaggtttt
    10741 gatctcggca gctaatcgct tcaactctct gattnnnnnn nnnnnnnnnn nnnnnncaaa
    10801 aaactgcaag agatcgtgat tgatgatgaa ctgcgccgcg ctgcgccaca ctgccggcac
    10861 gggcatgtcg ttgcgggcaa taccggccat cagttggtag ttatcggtat aaatatctct
    10921 gaaatcccgc tctatctggc gcaggctttt gccggcaatg atttgtaaaa tattgcgctt
    10981 ttcgtcttta aacaggtggt caatcgaata tttatcaggt ccaaaatact cctgcattac
    11041 cccgatgacg ccggccagat cgggcatgag aaaagcctcc cggatgcgct cctgcatggc
    11101 attgaatcgc tcccggctca tgttcagcga tatgttgccg atgatgtttt gctggcccag
    11161 atacaatgcc gcaaaactga aatgcttttc ggagtacgtc actttcgact tcaccgccgt
    11221 tcgcccgatg gccagacgca tgatgcccgc ttcgaggcgc tcaaaatcct cgctgtcggc
    11281 gatgtagttg aatagttcaa tgtgttcggg atacgcctca aacagcgacg aggccgcaat
    11341 gtgcatgccc acccgctgta gattgacgcg agccggctct atgtttcgga tgtagttgac
    11401 agcgccgctt tggtgtacat tactgggcgc atcggccagc atttttacaa acttcgttct
    11461 caaatcagta gcctcactca cctgcgctgc ataatacatg gcgcggtcgg cgtattgcaa
    11521 aatctggttg gtttcaatgc cagatatttc atcaaaaaac cagccgcaac tggtaaacat
    11581 gaggatggcg ttgcgctgaa tttccatcag gcgcatcagt ttcatccgct gttccgcact
    11641 cggttccttt tgtgcatgac gttgaatgaa ctcgttgaca gtctgatcat tgcgttgcag
    11701 caacacggag atgtagtcgt ttcgggccgt ccatacatcg cgcagcagcg gcccggcctc
    11761 tttttcgtaa atggccgcca attgatcgcg caattggttg agggcattgc gcagcggttc
    11821 acgccaggct tgcgtccagc cggggtgccc gccagaattg cagccgcagt tggagcgcca
    11881 gcgctctaca ccatgcacac agctccatga actgttttca tgaatttgcg cctccatact
    11941 tggcggaaac atctccaaaa actggccata attggtgatc cgggccatgc cgctttgctc
    12001 tatggtgtgc aggcaatcgg ccagcgccat ttcgccgtag cgatggtggt ggccgtagct
    12061 ttcgccgtcg gtggctatat gtaccaattg tggttcgata ttatcatcta aggcgtcgag
    12121 caggttggcg gcaaagtgct tgccgttgtt aaggatgcct ttaaaagcta cggcctgcga
    12181 caaatcgcca ttgtaaaaaa acagcgcaat gctgcgcccc gacggcaaca cacagcggta
    12241 gggccgccgg gtgtctaaat tgccctcgtg ggccgggcgc cattcgcttt cgcctgcgcg
    12301 gcgcacggag cgtacttgcc tgggcgccag cacggtaaac aagatgccgt tttcggccag
    12361 cacctccagt gtttccgtat ctgcggcggt ttcagccagc cacatacctt ccggcttgcg
    12421 cccaaagcgg tactcgaaat cggcaatgcc ccaacgcacc tgcgtttctt tgtcgcggcg
    12481 gttggccagc ggcatgatga tgtggctgtg tacctgcgcc atagccgagc cgtgccctcc
    12541 gaatcgcacc cggctcactt cgtcggcttc cagaatggcc cggtaagtgg cggggtcggc
    12601 atgttcgagc caggacagca gggtagggcc gaagttgaag ctgatgcgcc cgtaattgtt
    12661 ggtgatgcgc atgattttgc ccttcgtatc cagaattcga gcaacagcat tgggcgcgta
    12721 acattcaaag ttgatacggg cattccaatc gtgaaaaggc gcggcagtgt cttgcatttc
    12781 caccacttcc aaccaggcat tttcgcgggg cggctggtaa aagtggccgt gtatgcaaat
    12841 gaatttattg gcggtattca tggtcgtcgt ttgctgtgca gtgagtgggg caaagatata
    12901 caatccaacg ggccggggcc tgcgattttt gtactcagtg ccataaatca ctcaccccgc
    12961 tgactttcat cacctgtcgg agttttagag cggtactaat tttatccctg aataaaaccg
    13021 gtcaattatg aaaacccact ttccggacga gccgctgcgg tcacatacat tggacatgtt
    13081 gtggcaggct caggatgaga aagggtacat tgccaatgaa gacattgccc gaatagcatc
    13141 ccaactccaa atttccgaag tcgaactcga aggcgtagct tcattctacc atttcttcca
    13201 tcgaaaacct gcgggcaagt accgcatcta catcaacaac agcatcttag cggaattcaa
    13261 agactaccgc gacattgtgc gcgccttcga aaaagccacc ggcgcgccgc tgggcggtac
    13321 tgatcctacc ggcactttcg ggctttacga tacgccttgc atcggtatgt gcgatcagga
    13381 gccttccgcg ctcatcaatt tctacccatt caccaacctc agccctgcca aagtagatca
    13441 aatcattgac agcctgcgct ccggcgtggg ggtggagttc ttggccaatg ccgtcgagga
    13501 caacatccgc tacacgcccg aacgcgaaaa aaccatcctg ctgcgggagt tcgtgcccgg
    13561 caaggctttg caggcgctga cccggtggac gccaggagaa gtattgtctg aaataaaaga
    13621 atccggtctg gccgggcgcg gcggcgcgtt tttccccacc ggcgtcaagt gggagttgtg
    13681 cagcaaattt gagagcgatt gccgctacat cgtttgcaat gccgacgaag gcgaacccgg
    13741 cacttttaaa gaccgcgtcc tgctcaaccg cctgcccggc ctcctcattg nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt gccggggcat catttatctg cgggccgagt
    13861 accgctggct gctgccgaaa attcatgccg ccatcgaaat gtacgaacac ttgggatggc
    13921 tgggcgccga tacgactgcc aaagagccgt tccgcttcga tattcgggta caaataggcg
    13981 ccgggtctta cgtttgcggc gaagaaaccg ccctgctgga atcaatggaa ggcaaacgtg
    14041 gcgagcctcg taccaaaact tttttccctg tggaaaaagg ctatctgggc cgtcccaccg
    14101 tcattaacaa cgtggaaacg ctgtgcgctg ccgcccgcat catggagttg ggacacgagc
    14161 gtttctcctc gctcggcacg cccgttaccc gtggcaccaa gttgatcagc gtgggtggcg
    14221 attgcaatcg gcccggcatt tacgaactgg aatggggcat gacggtagcc gaactgctgg
    14281 agctttgcga tgcgcccgac ccttatttta ttcaggtcag cggcccttcc gggcaatgta
    14341 tcagcgcctg ggacagccac cgcaggtttg ccggcgaaga cctcctctgc ggcggcgcac
    14401 tcatgatttt cgactccacc cgcgatattt tgcagatcat ccgcaatttc accgcctttt
    14461 tcaagcacga atcctgcggc atgtgtacgc cctgccgcgc cggcaatttc atcttctccc
    14521 gcaagttgga caagatggcc cgcggcttgg gcagtctgca agacatcacc gaaatgcagc
    14581 agtggggcca gatcatgaag cgcaccaccc gttgcggcct cggcatgacg gcgcccaacc
    14641 cgctgctcta cgcgctggaa cgtttcccgg agtattttga acaaaaattg gacagcaccc
    14701 gcaccggctt caaccgcagt ttcgacctcg aaagtgcgct ggaagactac cgcgaggccg
    14761 ttcagtccta accacaaagc catggaaaaa aagctcgttc atctcaccat agatggccgc
    14821 ccgattgtgg ccgaagaagg ccagaatgtt gtggaagtgg ccaaagcaaa cggcatattt
    14881 attccttctc tttgttacta cccgcacatt gaccctccgc tgggttcttg ccgcgtttgc
    14941 accgtcatgg ccaacggcag atctgccgcc gcctgcacca cgctcgttaa agagggcatg
    15001 gacgtggtag tcaacaccga cgaactgcag gattggcgca aagccgtagt ggaaatgatg
    15061 tacgccgaag ggaatcactt ctgcccctct tgcgaaaaaa gcggcgactg cgatttgcag
    15121 ggcttgggct ataacctcgg cgtgacggta tcgcgctttc cgcacctctt cgttgatcac
    15181 gtggtggatt acaacccgca gcgcatggtc attgaacaca accgctgcat ccgttgccgc
    15241 cgttgcgtag aagaggtgct caccgacgaa ggtcaaagag tcttttcgtt ccaaaacagg
    15301 ggcaacacca ccgaggtcgg cattgactac gaacaggaag ccaaactcac ccacgaacaa
    15361 gccatgcagg ccatgcatgt atgtccggta ggcgccatcc tcgtgcgcgg caaaagtttg
    15421 gcccggcctc gcggcgaccg caaatacgac cacgataccg tcgtttcgga agtgcccttg
    15481 cccaaaatca ccgccctcaa accgcacctg cgccgcaaaa aaatagtggc caccacctcc
    15541 ttggccggct gcttcggctg ccacatgtcc atgcttgata tagatttaga cttggtggaa
    15601 ctcattgatt tggtggaatt taacaaatct ccgctcaccg acatcaaaaa attcaccaaa
    15661 cgcgccgacg tgggcatcat cgaaggcggc tgctgcaaca gcgacaacgt agaggtactc
    15721 aaggctttcc gcgaaaactg cgacgtgctc attgccatcg gcgagtgctc catctggggc
    15781 ggcttgccgg ccatgcgcaa ttttgtgccg ctcaaagaat gtcttgtaga agcctacagc
    15841 tcggccacca acaataccga gagcaaactc gtcattcctc accatcccga cctgccggaa
    15901 atattagacc gcgtgtatgc ctgcaacgag gtggtcaaaa tagaccattt cgtacccggc
    15961 tgtccgcccg atgccggcca tatctggaaa acggtgaaaa atgtgctgtt tggtgaaaaa
    16021 tacgccatta cgcacagcga gtttaagtat gactaggtag ctacgataga ctcgatgatt
    16081 aaatataaag cgctaaccca aaagccacaa gctgctacca cgccctggcg tggttttagc
    16141 ttgttttcac taaaggaatt agattttcct tatggggaat ttctgaattt gcttttgaaa
    16201 atgaattctt tttattttga aaaaccaagc taaaggcttg tggcaagtgg ctaacggctt
    16261 tgggaaatgc tgaataaata caatgaaaca aaaatgaaac aaaaaatcgt catagaaccc
    16321 gtaacgaggg tagagggcca cggcaaagtg accattcact tggatgaagg cggccgcgta
    16381 accgattcgt ttttccatat cgtagagttc cggggcttcg agcgcttcat tcagggccan
    16441 nnnnnnnnnn nnnnnnnnnc tgcggcatct gtccggtgag ccaccacttg gccgcagcca
    16501 aagccattga ccgcctgcac ggattagagc cggaagacct ttctccgaca gccggcaaac
    16561 tgcgccgcct cttgcactac ggccagattt tccaatcgca cgccctgcac tttttctact
    16621 tagcctcgcc cgacctgctg ttcggcgccg atgccccggt ggaacaacgc aacgtggtag
    16681 ccgtagccaa ggccaatcag gagctggcgc gcaagggcat tttgatgcgc aaattcggac
    16741 aggaaatcat caaagccatt gcaggcaaac gcagtcacgg catttgcgcg gtgcccggcg
    16801 gagcgtataa aactttcagc gaggcggagc gcgattattt cctcatgggc cgcgccattc
    16861 cctccattga tacgatgacc aactgggcca aggaaacggt agattttatc aaaaaatacc
    16921 acgaagacca cgccgccgag ttagatgaat tcgcgcattt tccttccggt cacctcggct
    16981 tggtgacgcc cgaaggcgac ctcgaactgt acaacggccg catgcgagcc atagatgcag
    17041 agggcaagat caccctgccg gacatccacg acagcctgta ccgcgagtac ttcgccgagg
    17101 ccgtagaacc ctggacctac ctgaaatttc cgtacctgaa aaacatcggt cgcgagcacg
    17161 gctggaaccg cgtcgggccg ctttcccggc tcaatgtggc cgaccgcatt cccacgccgc
    17221 tggcccaaca ggagtttgaa attttccgcg ctgccggcac gcctaatcac cgcagcatgt
    17281 actaccactg ggcgcgcctc attgagatgt tgcactgcgc cgaggtaatg aaagacttgc
    17341 tgttggacga ggaaatcacc ggcgacgacc tcctgcgcga gggcgaacgc caaccgcagg
    17401 gcatcggcat catcgaagcg ccgcgcggca cgctcatcca ctactacgaa gccgacgaaa
    17461 aaggcaaagt gaccaagtgc aacctcattg tgtccaccac gcacaacaac gagggcatga
    17521 accgcgccgt gcgcagcgta gccgccgccg agttggaccg ccgcccggaa attacagacg
    17581 gtatgctcaa taaggtggaa gtggccatcc gggcttacga cccctgcctg agttgtgcca
    17641 cccacgcgct gggtcagatg ccgctacagg tgcaggtgtt tgatcatgag gggaaattgg
    17701 tgcaggagcg ggtgaggtga ggaacttagg atttggtctc aaattctttt ttcaatgctt
    17761 caaacacctc cattcgtacc attctggagc tttttctgag tcgagccaat atattgaccg
    17821 atgtttcgct cagagtcgtt acattagctc tgccttgatt gcagttactg caaagaatcc
    17881 gcaaattttc tnnnnnnnnn nnnnnnnnna ttggtgccgc cttggctcaa tggaatgatg
    17941 tgatctatgt gcaggcgcac ttttttggta ggatcaagat cgtcataatc atgagcagaa
    18001 gcgccgcaaa tcgtgcaggt gaagccgttt cgctcaagaa tgaaatttct gagttgcaga
    18061 gaaatacttc tttcgatggc tggatcaaga tcaaggcttt ccaaaagata ttcccccggt
    18121 tttagatctg cacggtcaac attcgatctg attttcattc cgaattcatt tcgcagctcc
    18181 cggattcgcc gttggtagtc atttattcct gcaatcttgg caatctcgcc tgtggtgaca
    18241 actcgcccta cattctcttg aaaaaactgt ttgattcgct ctcttgcact cataatctaa
    18301 ttggttaaaa atgacagaat tgttgattgg agtaattctt tgtcttttaa attgcattgc
    18361 cacacgacca atacttccca gccggcttgc tccaaaagta gctggttttt tgcatctctt
    18421 gctacgtttt tattaaattt ggtttcccaa aacaaagtat tggaggatgg agtgcttgct
    18481 cttttacaac ccggatgcct gtgccagaaa cagccatgta caaaaattac tttttttcgc
    18541 cctgggaata taaaatccgg cttacaagga agcccgttat aattatagcg gtagcgaata
    18601 ccagattcga gcagcacttg cctgacgatt agttcaggct ttgtgcctcg attttttatt
    18661 cgggccataa tacggctccg tttggacggc tcgaatacat cagccattgc gctttatctc
    18721 cagaagtttt tcaactgagc gggctactgc tgcggccaac ataggaggta ctgcgttacc
    18781 gatctggcgt gcgatctcaa tttttgtacc tttaaaagca aacgtatcag gaaaagattg
    18841 aaaacgtgcc gcttctctat gtgtaatagg gcgatgctga gccggatgta ggtaacggcc
    18901 tttttcaggt ttgaaaaact ccgttcggat tgtaaaggac ggcctatccc accaaagtct
    18961 tccgaaaagg tctgtgccac ctgatttttt tcttttccaa caatccggtg tcaactccgg
    19021 cgcattcctt tccaaatcaa acctgttcat tccctcatga ggaattgcct tgtatctaag
    19081 tacgcttgtt tcggtcggag ttctgccgaa gtggatatta agtggtgtgg aatcaacaga
    19141 cggctcggtt cctatcggag gaggtaaatc agagatggcg tctcgtacag ttcgccaggg
    19201 gattgggttt tctgcatact cttccctgct gaacagatta ttaaggggcg atagacgtgc
    19261 cgacggatca aaatgtgttt tcgggggtgg aaacactttt ttgggatcgg caaatttaca
    19321 tcctacaata aaggcgcgaa aacgcttttg cggcactcca taatcagcgg cgcacaattt
    19381 ggcccaagcc atttgaaacc ccatagattc ggccgtttcc ataatttgtt catgctcagg
    19441 ggaacccaat atttgcggta cgttttccat cacaaaaacc tcagcctgtg aacgttctac
    19501 cacttccaaa aacggcatcc acatttgttt tctgggatcg ccctctctat gtttgttaag
    19561 taagctgaag ccttgacatg gcggcccccc gattacaaca tcggctttgg gtatgacggt
    19621 agaaggatcg tcaaggatag acagaatatc tccctcgtgg cagtgatcgc cgaagttggc
    19681 ccggtatgtt tcacaagcgt ctttgttgaa gtcattggcc caaaccgact caaaatgatt
    19741 tccgaatatt tttgtaaaac ctagtgtcat tcctccggcg ccggcgaaca aatcaatcag
    19801 cttataagaa gtatcccttt gtttgctttt ggaaataccg gaggacacct tctttttgag
    19861 gggttgttga atgtaagttg ctgattccat agttttttaa ttaccgggca aaaaatgaat
    19921 tcacaatatt catgtttttt catcaaaaca aacaaaacat ttcggaaaaa caccttctcc
    19981 cccaaatcag tttatccatc aaaacataaa ttgttctttc tccgccccac gcgaagcaga
    20041 gggccgttga acattttctt gtcattattt tcccaaaata ccacctaact ttgccgcctc
    20101 aaaatcaacg ttatgccgaa aatctcgcag cggggcgagt gtaccccgct ttcgccgttc
    20161 cgcaagtttg cacccttggc cgagcgcgcc aaagcacagg gcaggaagat atatcacctc
    20221 aacatcggcc aaccggacat cctcacgccg ccgcaggcat tgcaggccgt gcgcgaacat
    20281 ccgctccgcg tgctggccta tagctcggca gacggcaacc tgtcttaccg ccagaaactg
    20341 acgggctact accgtagttt cggagtagaa ctggccccca accaaatcat cgtcactacc
    20401 ggcgcatccg aagccatcta tctggggctg ttgagttgct tcaatcaggg cgatgaaatc
    20461 ctgtcgcccg agccatttta tgccaattac aacggctttg ctcacatggc cgacattgag
    20521 gtgcgcgcca ttccctgccg cattgaagac ggttttgccc tgccttccgc cgccgatttc
    20581 gagcgttgcc tcacgcccaa aaccaagggc atttttcttt gcaaccccaa caatcccact
    20641 ggctgcgtgt atcccgaagc taccctgcgc gaactggccg aggtggtcaa gcgccacgat
    20701 ttgtatctct tcgtggacga ggtgtatcgc gaattttgtt acgacgggca ggacttcttc
    20761 tccgcgctgc gcttggacga tatccgcgag catgtggtgg tggtggattc tatttccaag
    20821 cgttacagtg cctgcggagc cagaatcggg gcgctcatca cctacaacga acgcgttctg
    20881 gaggccgtca atcgctatgc catgctccgc ctcagcccgc ccggctttgg tcagatattg
    20941 gcggaagccg cccttgacgt ggatgccggc tatttggatg aagtaaaaat cgagtacgat
    21001 ctccgccgac aaacggtgtt ccggcgcttg cagcaaatgc ccggcgtaac gagctacctg
    21061 cccggcggcg ccttctattg ctttgcccga tttcctattg acgacgccga caagttttgc
    21121 cgctggttgc tcgaagactt tgaatatcag ggcgctaccg tgatgctctc gcccggcgca
    21181 ggtttttatg ctacgcccgg cgcaggcctc aacgaggtgc gcatggctta tgtcatctgc
    21241 cgcgacgacc tcgaagccgc catggactgc ctcgaacgcg cactcatgga gtatccgggc
    21301 cgagtagaaa ccgcccttcc cattttggaa gaagccatcc tatgagcagg caaccggctg
    21361 aagaacagcg cttagcagaa agctgggaca tcaacgcggc ggcctggacg caatcggtcc
    21421 gcgagggcct gattcgcagt cgccaacttg tgaccgaccg ggccatcacc gaggcagtgc
    21481 tccggcgccg ccctactcgc gtattggatg caggctgcgg cgagggctgg ctggcccgaa
    21541 aattgacatc gcagggcatt gcagtgaccg gctttgacgg cagcgaatcc ctcattgccg
    21601 acgcacaaca acacggcggc ggcccattct tcaccctcag ctacgacgac ttttgcgcgc
    21661 aagctcatgt cttgggagag ccattcgacg tggttgtgtt caattttgct ttgttgtcgg
    21721 aagaactctc gcccattttg cgcgcagcct cccaaacact cgctcccggc ggctgcattt
    21781 tgatacaaac cctgcatccg gtggaagcct cgcaaggcca aggctatgaa aacggctggc
    21841 gcgaggagca tttctccggc atgggcgagg ggtataaagc ggccatgcct tggtttttca
    21901 gaacgctgag tagctggttg gacgaactgc gccgggctgg gctggactgt gttgcgttgg
    21961 aggaaccctt gcatccagaa accgggcggc cggcatcgct gattctgacg gctgtacctc
    22021 gaccacaaat ctgatgcaac atggcaacgg agcataccac gataaaaatt caggacatag
    22081 aggttcctgt ccgcattcac cgggagatgc gcatgggtgc gagggccagt ttacgccagg
    22141 atgcagccgt gttgcggatt ccccggtatt tgcccgaatg ggaagaacag cgactcaaaa
    22201 agtggtttga ggactggctg cggcttcaga tggagtccaa gcctcatctt ttggagcgcc
    22261 ttcagtccaa ggttttttcc gatggcgaca tcgtaacggt gggagcgcgg caataccatt
    22321 tggccataga agaaagagat accaaaggac acagcgcccg gctgcggggc ggcgttattc
    22381 acattcaatt gagtcggtcg gggtcggaaa gcaatcagaa agaagccatt gccaccctgc
    22441 tgcggcgcat cgtggcgcaa gaccaattgc ccgcactcac cagaagggta cacgaactga
    22501 acgccctata ttttaagcaa aacatcaagt cggtgcggct caaatacaac cgctccaact
    22561 ggggtagttg ctcccgcagc ggcaacatca acctctcttc ccggcttctg tttgcaccga
    22621 cggaagtgca ggattatgtc atcattcacg aactggcgca tctactggag ttcaaccatt
    22681 cccaccggtt ttgggacatc gtagccgctg ccgacccaca gtacaaaacg catgtagagt
    22741 ggttgaaaaa acatgggcat gattgtgatt tttgagaaaa ccctcacagc aacaaactcc
    22801 cgtccccata actcaaaaag cgaaaatcat tggctaaggc ataatcatac atgcgccgcc
    22861 aatcctgccc ggcccaggcc gcgatgagca aaagcagggt gctgcgcggc tgatggaaat
    22921 tggtaatcaa tccgccggca atgcgaaacc ggtagcccgg agcaatgagc acctgagttt
    22981 ggccctccac aaaagattgt ccggccttgt ccaagcggcg caaaagttgc cgcaaaacct
    23041 cgacggcagg agcctcgttt tcttgcggtt cgtaaggcgt ccactggcga atgtgcaggg
    23101 tagtggcttc ggcggcagtc aggccgtgcc agtacaggct ttccagcacg cgcatactgg
    23161 tggtgcctac cggaatgacg gggcgaccct gctccaatac ctccgctatg cgcgcaatgg
    23221 tatcccgatt gacaatgacc gtttcttcgt gcatgtggtg ctctccgatg gtttccgact
    23281 tgaccggctt gaacgtgccc gcgccgacgt gcagcgtaac aaacaggcgc tccacgcccc
    23341 gtttgtccaa accctcaaat accctttcgg taaaatgaag cccggcagta ggggccgcta
    23401 ccgaaccctc ctggcgggca taaatggttt ggtagcgatc ttcgtcgtcg ggttccgaat
    23461 cccggtgcag gtagggcggc aggggcaatg ctccggctgc ggccaatacc tcgccgaagc
    23521 tgagcagggt aggggcctgc caggaaaagc gaacctcaaa agcgccttca cgacgttcca
    23581 atctttcggc gctcagggtt acaggccctg ccggcgttac aacctcgctt tgcagcaaac
    23641 cacctttcca gtgacggttg ttgcccacca aacaatgcca gatcaccggc tcgcggctgc
    23701 tcaggttcat ttggtactcg gcgggcgagg cggggtcgag gcagaatatt ttcaccatcc
    23761 tgcctgccgg ggtagtaaat cgcagtcgtg cgtggatgac gcgggaatca ttgaacagca
    23821 atacggcgcc ttgcggcaac tcctcggcca aattccgaaa taccgtttcc gaaatactgc
    23881 cctttcgata aaccaagagc ttggaggcat cgcgttccgg caagggaaat ttggcaatac
    23941 gctcatcggg cagcgggtag tcgtagtcct gaatagcgat gttttgcggc cctgccgccc
    24001 cgacttcatt cggtatattc atctgtagtt gtcgttgaag gtgcaaaatt gccgttttcc
    24061 gacggcacaa agtatcttgg gtgcatttat ccgtcaaaaa acagctatgg cgcaatgcag
    24121 gttcgtattt ttgtgaaacc attcagcatc gcccgattgg ctatttacga gatgttgatc
    24181 aaatacatgc cttatgaaac tttcacacat actgcttgcc ctgttattgc cggcggcaat
    24241 gattcggacg gcagcccaac ccgcggaggg cgggcttcct ttgggtcggg acattgagtt
    24301 tgcttccgat aagtcaatat ttacggtggc cgcccccgat gtggaacgcc tccgccagga
    24361 ggacctcagc cgctccggtt tccgtttcgc agcgcctttg gccgtcagca tttcacccga
    24421 aaacgcagga gaatggacta ccctgcccaa tggcgaccga atctggcgca ttcgcctgca
    24481 cgctcccgga gccaaggctg tttctgcttt gtacgataat ttttacttac ccccgggcgc
    24541 ccggctgttt atgtacagtc ccgacggcac gcagatattg ggcgcttaca cagatcgaaa
    24601 caacagcaaa accgggcgat ttatgaccgg cttcatcagt ggagaaacgg ctgtcgtaga
    24661 gtatttcgag ccagtttaca gatacgggca ggggcgtttt catatttttc gtttggacta
    24721 cgcttaccat tccgatccca atcaaataga aaaaagcgca ctggaaagca acaacttcgg
    24781 cttcggcgcg tcctgggatt gccaccccaa tgcaacctgc ccgcccgccg atnnnnnnnn
    24841 nnnnnnncgc agcgcctgcc gcattatcat agtggtaact gagggttccg gctattgcac
    24901 gggtactttg gttaacaatg tgcgcaacaa cggcgtaccc tacgtcatca gcggctttca
    24961 ctgcatggat ggctacacgc ctttgtggga cctttggagg tttgatttca gatatcagag
    25021 ttctacctgc acaaatccgg ccaccgagcc ggggtttcag tctatgctcg gctgcgacaa
    25081 ccgggccggt cgccgcgaaa atgattttct gttgttggag ctttttcaaa acattccggc
    25141 ctcttaccag gcttactttg ccggctggga tcggagtacc aatgtgcctg cccgttccac
    25201 ctacttccat catccgctgg gcgatattaa aaaaatcagc gtggacacgc aggcggccgt
    25261 catccaaaac acgcccattc agtggaacaa taccgtgact acccccaccc agcatcattt
    25321 cagggtacga cccgacatag gctcgtttca ggtaggctct tcgggggcag gcttgtttaa
    25381 tcataacaag cgctttgtag gccaattgca cggtggattc tctacctgcg acagtacggt
    25441 ggcctacttc gggcgcatgg ccttagcctg gaacggcggc ggctccccca tcacccggct
    25501 cagagactgg ctggaccccg acgataccga ctctttgaga attgcaggta ttgaaaatcc
    25561 ggcggccggc tcggtcatta cccttacggg gacggttcgc accgaatctg gtatgcccat
    25621 tcccggcgtc agggtttggc tggagggcat gctcgccgat acggctacta cgggggcttc
    25681 gggcgagttt accttcccgc tgctagatgc agggatggac tacactttgg gatttttgaa
    25741 agacacccac cacagcaacg gcgtctctac gcaggatgtc atccgcattc aacggcaaat
    25801 tttgagcatt gaaaacctca acagcgctta caaacagttt gccgccgacg tgaaccaatc
    25861 cggcaatgtt tctactttgg acgttattct cttgcggcgc attattctga ccatcatgga
    25921 caaatttccc gatgtccgct cctggcagtt ttttccggcc aatcacactt ttgccgatcc
    25981 gctacatcct ttcggcgaac tgacgcccaa tcgcgtgccc ttgggcatgt tgaccgggag
    26041 catttccatc aatatcacag gcgtaaagtc cggcgacgtc aacgaatcgg ctgatccatc
    26101 caattgaatt gaacgctatc tggcaagcac cctttgggtc agcgtccctt tgtcggttct
    26161 gatttccaac aaaaacacgc cgcgacccgg caaggacacc tctgtggctc cggcaggaag
    26221 gcttttcagc caactccctt tggcgtcgaa cactctgatg cccagtacgt tcagcccgtc
    26281 tgccagaacc tgacacttgc cgtcgggggc cgggttgggg aacactcgaa tcgcctgttg
    26341 cagtgccgac tcccgcgcag ccgtcacacc gtccacatac acattgcctg tttgagccga
    26401 tgagacagcc accggagagc ggtcggcatt gttgaacatg gtgcggaatg tttctatttc
    26461 gggcgtggaa tctgcaaaaa tttcctccat ccatttgggt ggcaaagact ggtaatatac
    26521 cgtagcataa gccctgatat tgccctggta gccgttgacg ctgatgcggt aacgaacgat
    26581 gtcggagccg gtaccttcta ccccgttcac cttattaaag tcagggtcgt tcaatgcgtt
    26641 gccgaatatg cgagtggtat cgtaagcgct gtgcgtggtg cggaagccca atggcggcag
    26701 gcgattgtct ttcagcggct cgtagcctct gagcaggaca gtagtcagtt gacccgcagc
    26761 atcgcccatc accatttcgt atatctgcgc ctgatcggga cgggtaatca tttgataatg
    26821 aggctccacc accggatcat ggccctctac ctcatagtcg ggctgcaaca tacctgagcg
    26881 aaaaagggtg tcgccttgtt cggtaagggc cacaaaactc accaatgccc gcctgctggg
    26941 gtagccggaa gggaatttgt gcccggcgcg gttggtgagt ttcagcgcaa aagtgacgga
    27001 atcggagcgc acggtttctg cctgcaactc catgctcagc gtcttatcct gcagcatggc
    27061 ataagtggct gcaatggtgg cgtcgtaatt ttcggcggan nnnnnnnnnn nnnngcccaa
    27121 agcctggcga ttttgtttca tcaggcgcag catcatggtg ttggcgccgg ccatttcgtg
    27181 tatgccgaaa ggcacgcgcc catccaaaaa caggtagttg gaagaaatga cgacggggtc
    27241 tttgatgcgc gaaaaatggc agtcctgaca ggtcacattt tgcaatccgt aagtagaatt
    27301 gagccactca tggtaggtgg cctgctctac gaaggcaatg tccaaaaact cgccctgcgc
    27361 attcaccgga tgggtaataa gggtgtggca gggcgcacag aggcccgctt cgggcacatg
    27421 ctggccatgc aagggcctga accctacaaa atcctccatc ggcgctgcaa agggttcata
    27481 ataagggcca tatatcacac gggctgtatc gaaattgatt tgtccggaga aggtcagccc
    27541 aaaatcctgc tgactgattt tgtggcaggt gctgcaactg acaccgtcca tgccgataga
    27601 atcggccagc atatcggcta tggagtaagg cccgtgcccc cggtaatgcg ccgtgtaacg
    27661 cccgttggga gcatggcagg aagtacattt gtcttgaatg gctgcgctgt gcataggatg
    27721 tatcaaaatc tcgtggctca ccttggcccg ccagaagggg tctttggcac tattggccat
    27781 catggtagcg ctccaatcgt ccactacatt gacgtcgtta ccggctaaat ctaccgaggc
    27841 gtaaccattt tcatctctgc cgtgacaacc cacacaggcc ttcgaggtgg ggaatatgac
    27901 gttggaatca acgacttcca gcatagagtt ggcatgaaaa gcctgtattt cctgcggagt
    27961 atgaaagcgg ggtggcccgt aagaggtcca ttgtgcagcg ctgtaaatga gaccgaggag
    28021 caagcctccg aaaatgaaat gtttggatag tccgggcata acggcaattt tttgctaaaa
    28081 cttgaacagg caaaattgca aaatgctccg gtaatccacc tcagcgaaag aggaaaacat
    28141 tgtatctcat tagacaaaaa tagattagga ccccgttcct tagccccaca tcggcgaggg
    28201 gtacttgcct tcctgtgcaa attgtgcaaa cgccttttag gcaatatctt tgtcttcgcg
    28261 gtaagtgaca ccgtaccatt gctcttcgtt gggcaataca gataccgctg cacggccttc
    28321 gctcaaaagt ctgttgacca ccgtgggaat gtaaaattct gctttgggat tgtttttcga
    28381 ggcttcgaag aagtctaaaa actgacgctc tatctccgta aaaatttgta ccggcaggcc
    28441 ccataaattc atggaaacaa tggcgttgtc gtccaatgga ctggggtttc catgctcatc
    28501 ggcaaaaacg atgccggtgt cggtgcgctc aattttgtgg cgttcggtca cgcccacgag
    28561 ttgccctttg tcgttcaatt cgcatacgcc tctggagacg gttccatgct ccgacaaggt
    28621 atttttgatg cgatagccca ccatagcgag gtgcgaggcg tggcaatcac gggtcagaaa
    28681 ttcagccatt ttgtaaaacg catcggcgcc gtaatagtcg tcggcattca ctacagcaaa
    28741 aggttcctga atcacatctt tggccatgag caccgcgtga cctgtacccc agggtttttc
    28801 ccggctcggc cattcgaaaa tgccggctac ggggctgtct gtttcctgaa
//
LOCUS       DAORLF010000008        28413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_920_length_28413_cov_2.234890, whole genome shotgun sequence.
ACCESSION   DAORLF010000008 DAORLF010000000
VERSION     DAORLF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28413
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_28413_cov_2.234890"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PK198_01615"
     CDS             complement(<1..561)
                     /locus_tag="PK198_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF37457.1"
                     /translation="MVFLDFEKPLETLYEQLDKLRQVGEDGELDVTEQIKELESRIRN
                     KRKEIYSNLNGWQKVQLSRHPERPYTLFYINQMCKKFVELHGDRCVKDDKAIVGGLAS
                     LDGQPVVILGHQKGINTKMRQYRNFGMANPEGYRKALRLMKLAERFNFPVITLIDTPG
                     AYPGIEAEERGQAEAIARNLIEMAQLR"
     gene            complement(573..1352)
                     /locus_tag="PK198_01620"
     CDS             complement(573..1352)
                     /locus_tag="PK198_01620"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRF37458.1"
                     /translation="MSATIQAKEETYKKVKTILVSQPKPERSPYYDLEKKYGLQIDWR
                     PFIHVEPVSAKEYRKQRVYPDEYTVIIFTSKYAVDHFFRLCDEMRIKMSQETKYFCVT
                     EAVANYLQKFIIYRKRKVFAGNKSIEDLTSALKKHRNEKFLLPCNNLGSQDVTAYLKE
                     LGIQYQDALMYRTVSSDLSDLSDVTYDMLVFFSPQGIESLFDNFPDFKQNETRIAVFG
                     NSTLKALEEKSLVANVKAPAPEAPSMATALELYLQKSNPLT"
     gene            complement(1392..>2347)
                     /locus_tag="PK198_01625"
     CDS             complement(1392..>2347)
                     /locus_tag="PK198_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRF37459.1"
                     /translation="RQINGNCIFAAIFKINPLKAFAWILVLTLATIAPVWGQGMLNPF
                     ELKPRLAPSETAPPAWNTGNPFDVAHAVNPVAAGAVQPVNKAKAKPAAKPPRPRLTTQ
                     EQYKTFLFAITVANLALLTLLITIFRGVYQKAYRGFLNENMLNQIHREAQSSGALAYY
                     ILYIMFVLNAGTFVFLVARHFGATFPWGLWQSWLMCVAGTAALVLAKHLVLTYIGAVF
                     PVDKETSNYQFTMVVFGILLSLVLVAANVFVAYGPSEMTTPALFATFGITGLLYAFRS
                     LRGLLIANNFLATHKFHFFLYLCAVEIAPLLVFVKLIQNQA"
     assembly_gap    2348..2369
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2379..>3646
                     /locus_tag="PK198_01630"
     CDS             2379..>3646
                     /locus_tag="PK198_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF37460.1"
                     /translation="MKNSTIFRVILLGVVAIAGIIAIQSYWVMSSWNLNEDEFQKKVN
                     LALYNTARSLADLNGHTLPARNVVRQRSGNYFVVNIAYEINTEDLEFFLQRELEELAL
                     NIDFEYAVFDCETDEMMYGDYVSYSPNQNSQAKTGELPKHSEFTYYFGVRFPTRTDYL
                     LNRIQLSILFSVILFVAVIFFAYSMAVILKQKQLSETQKDFINNMTHEFKTPISTMGI
                     SADVLLNSPAVQAEPRLLQYAKIIKEQNQRLNHQVEKVLQLARIEKGNFQIKPEKMEL
                     DAFLEQAIQGMQIQAEQKGGAISGLFHASGCIIQADRLHLTNVVNNLLDNALKYCADK
                     PQVIVTTICNKGRVTLSIKDNGIGIAKENTKKIFHKFYRVPTGNVHDVKGFGLGLYYV
                     RNICQAHRWQLRVESTPGAGTDFQISIPLAP"
     assembly_gap    3647..3672
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3673..4338
                     /locus_tag="PK198_01635"
     CDS             <3673..4338
                     /locus_tag="PK198_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37461.1"
                     /translation="VEDDESLSFVTRDNLEMKGYRITHCANGREALEVLRNQSFDLCI
                     LDVMLPEVDGFDIAREIRRLDQHTPIIFLTAKSLKEDRIHGLKIGADDYITKPFSIEE
                     LILKIEVFLRRSKGATSSAPTLYRIGEYEFHPQNLTIERAGDSRRLTQRESDLLLMLV
                     QQKGQVVKRSLILEKLWGQDDYFLGRSLDVFISRLRRYLGADPTIKLETVHGVGFQLL
                     TDM"
     gene            4400..5737
                     /locus_tag="PK198_01640"
     CDS             4400..5737
                     /locus_tag="PK198_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRF37462.1"
                     /translation="MKKTKGAYRIYLLTALSAAGYFALGQTGRTHFSTLLALYIVLFV
                     LFLLLVRQIKMADAVRFGIGCSLVLQLVLLFHLPNLSDDYFRFIWDGQLITHGINPYS
                     ALPPEVLDVIAPTAPMSEELFARLNELQRSNYTCYPPFNELFFVLPAWIFPQSILANV
                     VLMRLFIIAANLGTALLGLRVLRALGRPESDVLYFALNPLVVVELAGNLHFEALMVFF
                     LVAALHFAVRKRHLSAGAALALSASVKLAPLMLIPLFFRFFGFRKWTAFAAVTVGLTL
                     LLFLPVPVLGGVEGFFKSIGLYFNNFEFNAGIFYGLKQLGYWFSGRNMIAVIGPALSV
                     VTLGIIFYLTFFRKNEAPGTLISSLLLAMTAYYLLGTTVHPWYIATPLALSVFTSFRY
                     PMVWSFAVFFSYSAYAATPFSENRWLVGLEYLFVLAVLLWEWRKREEPVPERR"
     gene            complement(5749..6696)
                     /locus_tag="PK198_01645"
     CDS             complement(5749..6696)
                     /locus_tag="PK198_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF37463.1"
                     /translation="MKTDFNHCESLYWVFTQLCNDQCDHCYNRSSPKGARISEEECMT
                     IIENMPDRVDRLILSGGEPLADRKLLYAILDRLRDRYAERTQIMLQTNGDLLTEPILA
                     TLIEKGVSRFDIASIDRYHKHAGARLEVLAELFEAHGVRGDHKDPLIDKDSYLIENEL
                     SWGYWGATEDMWLGGNWARGMALEKGIWKRDPQHNFCTILSGARNFLGGYDDIPQEIS
                     IQLWRINPCCPGTKFPIGDARTERVADVLERVSQHPVFKKLNEGDPWRMGEHLGISEA
                     EARKRTEVLQNVCLYCDAFFEENMQALNRERFIELPVLQ"
     gene            complement(6693..7418)
                     /locus_tag="PK198_01650"
     CDS             complement(6693..7418)
                     /locus_tag="PK198_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF37464.1"
                     /translation="MFLQVSNLSKTFGKEQVLRNLSFALDAHRMMSVLGRSGSGKTTL
                     LKIIAGLESPDNGRVELEGKAMNETPAHLRNIVYLYQEPLLFPHLNAFENIAFGLRLR
                     KWTEADIRQKTETMLDHLELNGQAKKMPAQLSGGQRQRVAFGRALIVEPKVLLLDEPF
                     GALDTETRTAMQQLLKRMVAGSQITTIFVTHDLKEALLMGDEIALMQDGLLKKYETKA
                     DFVQDARTGVQEEIGFWTNIQSR"
     gene            complement(7421..8182)
                     /locus_tag="PK198_01655"
     CDS             complement(7421..8182)
                     /locus_tag="PK198_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37465.1"
                     /translation="MLKTMLKYTFAGAVLFPFALLLVFSFGQNWRFPALFPALWTMDN
                     WQLLLTSQSDLAQSLLRSLSISAGIATAATWLGFITAKHIAYHPWRQQWLTLAYLPYI
                     FAPVILAATLQFFFLRLGLAGNVPGVLFAQLLIAYPFGVIFFTGFWNDRMRAMEQLSA
                     TLGGNKRQTFLRVLLPAAQGALLVCFFQAFLISWFEYGLTTLIGIGKVQTLTVKVFQY
                     IGEANIFHAALASLLLALPPAGLLWVNKQVLFKTS"
     gene            complement(8176..9039)
                     /locus_tag="PK198_01660"
     CDS             complement(8176..9039)
                     /locus_tag="PK198_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF37466.1"
                     /translation="MAGIYKGKYASLGLFLALTALPIALGIGYALLRSMGLAGLGAEG
                     FTLRHWEAVLSGGEFWRSLGLSFRVAGCSIGLAGVLALAAVLKAPERWQRGGLSWLMY
                     LPLTLPAMVVAFVVFQMLSGAGLVSRLAYKLGLTSGIQDFPNWVNDSWGIGIIAAHVF
                     MATPFFIILFAQLYQSERLGEYAQLAATLGATARQSARRVIVPVLLRQSFPTLVLYFI
                     FVMGSYEIPLLLGSQSPQMVSVLTIRKLQRFDLADIPQAYAIGVLYMVFVAAAAVFLL
                     RNNAEKTSSTC"
     gene            complement(9023..9613)
                     /locus_tag="PK198_01665"
     CDS             complement(9023..9613)
                     /locus_tag="PK198_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRF37467.1"
                     /translation="MRDALIIFVKNPVPGQVKTRLAATMGHETALQIYRALLHRIRQT
                     TEPMPVAKYLFYSDFIEKNDEWPNDLFIKKTQCNGDLGERMGNAFEEVLRLHPRAVIV
                     GSDVPGITSGIVQEAFEQLAGHDFAIGGTDDGGYYLLGMSEYEPSLFTGIEWSTPLVF
                     SQTMEAMGRLGKSCALLPVLPDIDYEEDWTKHGWDI"
     gene            complement(9617..>10556)
                     /locus_tag="PK198_01670"
     CDS             complement(9617..>10556)
                     /locus_tag="PK198_01670"
                     /inference="COORDINATES: protein motif:HMM:NF008633.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF37468.1"
                     /translation="EMEAGKSVGELDLMWINGETFYQLRQIDALFGPWTERLPNAQFI
                     DFQNPFINTDFQQPVNGYECPWGNVQMTLIYNSEFVKDPPRTRAQLADFVKAHPGKFT
                     FDTQFTGLTFLKSLLIDIAGGGNALDGPFDEAKYGQYSAQLWDYLRDIQPYLWKQGKT
                     FPDSRSPMHQLFSNGELWFTLTNNDNEVDSKVGQGLFPPTARAYVPDFGSIQNSHYMG
                     IPKLSGNKAGAMIVANFLLSPEAQWEKQKQEAWGDGTVLDPSKLPGDWKQRFENLAAR
                     KYAPSRAEMQSRALKEPAPEYMIRLAKDFREQIIER"
     assembly_gap    10557..10630
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10631..10849)
                     /locus_tag="PK198_01675"
     CDS             complement(<10631..10849)
                     /locus_tag="PK198_01675"
                     /inference="COORDINATES: protein motif:HMM:NF008633.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37469.1"
                     /translation="MQRFLLLAHLFLTACNNPQPSHEADPTRLPWEQVIEQAKGSTVQ
                     LMMWQGDPLINAYMQQYVAPAVKQQFSIT"
     gene            complement(10937..>11617)
                     /locus_tag="PK198_01680"
     CDS             complement(10937..>11617)
                     /locus_tag="PK198_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37470.1"
                     /translation="GSQVFGVARNVVMSETEGESRYYGASFNLQQDRGDGKLAWRINY
                     TLSYLENNTEDINFRAMDGNDFEAEWGPSINDRRHIFNGILSWFPTGGLGVTLAGLVQ
                     SGQPVNRIPDALLYGTTDLNGDGASFGDAYVGNSDRHPGESRNSDRLPWSKVFDLGLQ
                     YKLPVGKHQLELRADVFNILNTVNLSGYSNNATQSNQIQVGGRARGIVEKNAGAPRQF
                     QFGARWLF"
     assembly_gap    11618..11685
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11686..>12083)
                     /locus_tag="PK198_01685"
     CDS             complement(<11686..>12083)
                     /locus_tag="PK198_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF37471.1"
                     /translation="ALMGENGAGKSTLLNILSGVQPPTSGRITFDGNLSGSTANVEYL
                     KGPGFGSLQGQREGVFSNERRILNPNGYDNPLTRQMALGYQLQWNEKRLFYVDLVHNR
                     SQNLFRLRNLNAAAAYPIANPDNVTVRTPA"
     assembly_gap    12084..12137
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12138..>12440)
                     /locus_tag="PK198_01690"
     CDS             complement(<12138..>12440)
                     /locus_tag="PK198_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37472.1"
                     /translation="QLSQLRESALGSNLNVNDIPADVQVLNYNVELRPASFGKTQNIL
                     SLYLEDQFTLSDRLNLTLGLRYDYDNLSKGGSEQGDLNNLAPRFNFTPKRPDSGPVE"
     assembly_gap    12441..12546
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12547..13901)
                     /locus_tag="PK198_01695"
     CDS             complement(<12547..13901)
                     /locus_tag="PK198_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37473.1"
                     /translation="MRLFLWMILTLFVTTLLAQEDLTVSVLDPQNRPVANAPVQLKNA
                     TWGIEQTTETNAQGRAVFRSLPVVDGYRVLIPDFLPYQAAQSDLINLRSNENRTVQMV
                     LSETTVSLGEVTVRAASSAKINRTDAEVSFELSKKEIEALPIEGRDITRALYRLPNVS
                     QATGFYPEAPNVSINGANPLFTSYLIDGLDNNERFLGGQKFAMPVGFAKDITVLTNNY
                     SAEYGLTNNGVVNITTRSGGNEFSGEAFIVNRPGPALDARSPFAQRDLSGNFVKDGFQ
                     RYQAGLGFGGAIRKDKTFYYLNLEHTTDLKDNLLNSPALGVNETVRGKNRFTYLSGKI
                     DQIWSSKFRSSIRANVGMVAIERQGGGLEGGVGFPSSGNFQDRNSLLLASKNTYVSGR
                     FSSETNLQYARFRWNYGRAANEGSPQVVVLDPSEQAVAVLGHPGYIFDATENTVQLLQ
                     K"
     gene            14218..14958
                     /locus_tag="PK198_01700"
     CDS             14218..14958
                     /locus_tag="PK198_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRF37474.1"
                     /translation="MFKTLSFLVVFMVASANLAAQKPVQHDIWDALLKKHVRKDGFVD
                     YKGFVKDSARLNQYLEVLSSAHPQSSWSRAEQMAYWINAYNAFTVKLIVQHYPVASIK
                     DIKKGIPLVNSVWDIKFIHIQDKTYDLNNIEHDILRPQFKDARCHAAINCASYSCPRL
                     HNEAFTAAKLEQQLEAVTRSFVNDPLRNRIKADRAELSAIFNWFGGDFKRDAGSVRAF
                     VNKYAQVKVKDTGKITFIDYDWSLNDVK"
     gene            15598..>16542
                     /locus_tag="PK198_01705"
     CDS             15598..>16542
                     /locus_tag="PK198_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRF37475.1"
                     /translation="MGLFSFFRQELAIDLGTANTLIIQDDKVVVDEPSIVAIDRKTNS
                     TIAVGKRAMQMHEKTHEDIKTVRPLKDGVIADFQAAESMIEGLIGMIGKRRRFFSHLK
                     MVICIPSGITEVEKRAVFDSADHVDSKETYLIHEPMAAALGIGLNVEEPVGNMIIDIG
                     GGTTEIAVIALSGIVCDQSIRIAGDELTNDIVSYMKRQHNILIGERTAEQIKINVGSA
                     LPELEDGPEDYAVNGRDLMTGIPKQIKVGYAEIAHCLDKSIAKIEDAVLKALELTPPE
                     LAADIYLTGLYLTGGGALLRGLDKRLSAKTKLNVHIPED"
     assembly_gap    16543..16558
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16680..17582
                     /gene="mreC"
                     /locus_tag="PK198_01710"
     CDS             16680..17582
                     /gene="mreC"
                     /locus_tag="PK198_01710"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRF37476.1"
                     /translation="MRNLLEFLARFGGFFLFLILEALSFFLIVRFDDDKNRIFLSSSN
                     AVAGYMLKKYDAVADYFSLPGELDQLQEENKRLREQLAESYYAPDYDVDTVSLTVRTQ
                     DSIYSKGVVVRDTLQKQDSMATQIYAYVEANVISNSINRRNNTLTIDRGTSQGIAPRM
                     GVIGPQGIAGIVKTVSKHYASVVSVLHSDISVSATVKHKGYFGALVWNGPDPRYMQLE
                     AIPKHAEIAAGDTIVTSSFSGVFPAGLLIGIVESVTQNPGDNFHNIKVALASDMSRTG
                     RVYVVQNKWLEEYKQVQKQEEDRK"
     gene            17579..18100
                     /locus_tag="PK198_01715"
     CDS             17579..18100
                     /locus_tag="PK198_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37477.1"
                     /translation="MSGLVITNGIRFLILLIVQVLVLKRLSPGIESFNYIHILLYPLF
                     ILLLPLRTPQALTLGLSFMLGLMVDVFYDSPGVHAAASVFTGYLRGLVLGYMEPRGGY
                     NVNFSPTKERMGFRWFAGYAAVLMAGHLFFYFSMEAFTFAYFADTLLKTLFSFVVSMG
                     FVLAVMFVFNPES"
     assembly_gap    18260..18324
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18325..19981
                     /locus_tag="PK198_01720"
     CDS             <18325..19981
                     /locus_tag="PK198_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF37478.1"
                     /translation="PIYDLMVTYSQIKPGMDTSHFCQLLGITPEYFKENLTKDWRSGR
                     FSKNVPFVFLSKVSAETYARFQENMFDFPGFQMVLRSIRGYPHQNAANVLGYIREVDS
                     RDLEKYPGLYAPGDYIGASGLEASYETLLRGAKGVSYILKDNLGRNMGPYKDGQLDSA
                     AVSGADLITSLDIDLQAYGELLMQNKIGGIIAIEPSTGEILAMVTSVSYDPNLLTITQ
                     KRGVAFAALNRDPLKPLFDRTTMAQYPPGSVFKSILGLIATQEGVWNINRGVGCGGGY
                     VLNGQRLVGCHGHPYCSNLEMAIQHSCNAYFVTVYRELIDKYGFYNPAKGLDELNEYL
                     VRFGLGAPLGIDLPGERGGYYPTTAYYDKTYANSGGKWNSVWIRSNGIGQGEHLMTNV
                     QMANLAAIIANKGYYITPHLLKGFKSGGSEIPELYREKKSVGIDAQHFPPVINGMEKV
                     TISGTARSAFIPHIPICGKTGTAENPHGKDHSVFFGFAPKENPKIAFAVYVENSGFGG
                     TYAAPIASLMVEKYLTDTISAPRKFLEKRMLDANLLQFQKTKP"
     gene            19944..>20988
                     /locus_tag="PK198_01725"
     CDS             19944..>20988
                     /locus_tag="PK198_01725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRF37479.1"
                     /translation="MPICSNSRKPSLNALWLFIAVAFPLFLQAQSDNVRRLKLIFAGD
                     IMGHETQIRAAEVEKDKLYDYTPCFEYVAPILKQADLAIGNLELTLPGKPPYTGYPMF
                     KSPEELALALRHAGFDLLVTSNNHSNDGGLNGVINTLNTLDNYNFYHTGTFRDSLERN
                     DFYPLIVYKNDFRLAFLNYTYGTNGLITKPPSVVNLIDEQLIAADMETARQMAPDFII
                     VIMHWGDEYQIIENKTQRDLAAKLIGWGADLVIGAHPHVVQPVRQETAAGADSTGRKV
                     WTAYSLGNYISGQRKINTDGGMMLEVELQKTLPAGPTALTDIAFIPTWVWIQTLPTGK
                     RHYRILPVAAFEQE"
     assembly_gap    20989..21155
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21319..>21971
                     /locus_tag="PK198_01730"
     CDS             21319..>21971
                     /locus_tag="PK198_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF37480.1"
                     /translation="MKATRILWLLMAISIIAYPSCKTNRTVKGAVIGGTVGGVAGGAI
                     GKKSGNTAVGIIIGAAIGGTAGALIGNYMDKQAAEIRRDMKGAKVERVGEGILITFDS
                     GLLFDVNSYELRSTTRQNLNELAKVLGKYDDTDIVIQGHTDNTGGTELNQTLSENRAR
                     SVKSYLMQQGIVISRMYDMGMGENYPVQDNATAAGKQANRRVEVAIYANKKLKKAAKR
                     "
     assembly_gap    21972..21999
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22060..>22344
                     /locus_tag="PK198_01735"
     CDS             22060..>22344
                     /locus_tag="PK198_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37481.1"
                     /translation="MQAYEIKLLETALILGAFLVARLILSKFFLRVNRIFPAQKNRID
                     GFRKILNLILGAVSTVLVFIVWGVDQKELLLFVSSFLTVLGIAFFAQWSLL"
     assembly_gap    22345..22385
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22386..22580
                     /locus_tag="PK198_01740"
     CDS             <22386..22580
                     /locus_tag="PK198_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRF37482.1"
                     /translation="KGGDTIMILDKDFSAQGLIREIGIFFIHIETTPGETITVPTSLF
                     LQKMVKIVEQSDASKASSKE"
     gene            22577..23626
                     /locus_tag="PK198_01745"
     CDS             22577..23626
                     /locus_tag="PK198_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRF37483.1"
                     /translation="MTSTTFTDLLSRLIATPSFSREEHVTAELLADFIHNQGIAFRRK
                     HNNIWAANLHFDPAKPTILLNSHHDTVKPNSAYTLDPFEPLVRDGKLYGLGSNDAGAA
                     LVSLLAAFLHFYKRENLTHNLLFAATAEEEISGLNGVESILPELGDIALAIVGEPTLF
                     QMAVAEKGLLVLDCTAQGKAGHAAREEGVNAIYEAMSDIEWFRSYRFPKVSPTLGPVK
                     MSVTVIQAGTQHNVVPAECRFTVDVRCTDAYSHEELLDIIRSQVRAEVVPRSMRLRPS
                     AIPMEHPIVQAGLVMGLSAYGSPTSSDQALMPFTSIKIGPGDSARSHSADEFVYLHEI
                     EQGIAGYIRLLEQVL"
     gene            23709..25016
                     /gene="argH"
                     /locus_tag="PK198_01750"
     CDS             23709..25016
                     /gene="argH"
                     /locus_tag="PK198_01750"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRF37484.1"
                     /translation="MMKLWQKEGNEAGEWSRIVETFTVGRDLEFDILLAEHDVLGSLA
                     HTQMLQSIGLLTAEELASIHSGLNAILEDIRAGRFSLEPGVEDVHSQVELQLTRSIGE
                     TGKKIHSGRSRNDQVLLDIKLFLRASLRQTAAHIQALFERMQSLSEAHRERLLPGYTH
                     FQIAMPSSFGLWFGAYAESLCDDMELLLAAFRIADKNPLGSGAGYGSSFPLQRKMTTE
                     LLGFSDLNYNVVYAQMTRGKAEKAVAMALSSVAATLSKLAYDVCLYMNQNFGFITFPD
                     ALTTGSSIMPHKKNPDVFELVRAKCNRIQALPNELTLLTNNLPSGYHRDLQLSKEILF
                     PAIAEMDNCLEMTEFMLRHIIVKDGILDDEKYLHLFSVEAVNELVLAGLPFRDAYRQV
                     GQAIEKGEFHAPAQLNHTHEGSIGRLCNEQIRVRMKGLMRSLG"
     gene            25129..25614
                     /locus_tag="PK198_01755"
     CDS             25129..25614
                     /locus_tag="PK198_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37485.1"
                     /translation="MMLKIKLLLLLSMVALFACIDRGKDIADKNLSAPFPPIDHYYLT
                     GQQPFSGEDLVSRGRITGFHKATGLVHSYTLVYDIIKRSYSLTHYGTAEDGKYLKRPI
                     DFILADSILTNINEAFVHGTIKRGDTYLDTPHSDYLSLTIEVSIGEAEPMSAEYFFLR
                     F"
     gene            25796..26908
                     /gene="ald"
                     /locus_tag="PK198_01760"
     CDS             25796..26908
                     /gene="ald"
                     /locus_tag="PK198_01760"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF37486.1"
                     /translation="MIIGVPKEIKDNENRVALTPAGTRELTKRGHTVYVQTNAGFQSG
                     FPDAEYTQAGAVILPTAEEVFQKAEMIMKVKEPIAQEYPLIRPNQLVFTYFHFASYEP
                     LTKAMIQSGAVCLAYETVELPDRSLPLLVPMSEVAGRMAIQEGAKYLEKPQKGRGVLL
                     GGVPGVAPGKVLVLGGGIVGTQSAKMDAGLGAQVTILDVSLPPMRYLSDVMPANVITM
                     YSNEYNIRRLIRDHDLIVGAILIPGAKAPKLITRDMLKEMRPGTVMVDVAIDQGGAFE
                     TSKPTTHADPVYIIDDVVHYCVANMPGAVPYTSTIALTNATLPYAIELAGKGWQKACS
                     DNAALKLGLNVVGGKVVYQGVAEAFDLPYTPVEEVM"
     gene            26930..>28413
                     /locus_tag="PK198_01765"
     CDS             26930..>28413
                     /locus_tag="PK198_01765"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRF37487.1"
                     /translation="MRVFFELNALPEFRNAVLTIGSFDGVHAGHQEILRRVIRLARQH
                     KGESIVVTFHPHPREVVYPQDDRPLRLLTTIRERIELLEQYGIDNVVVVPFTVEFSQQ
                     SADEYIQKFLIESFRPKFIVIGYDHRFGLNRQGDINFLRWHGEQGGYEVVEIEQQIID
                     NNAVSSSKIRAAVERGDMPVARTLMEHFFQLSGPVVRGQNIGMELGFPTANLDIPAKN
                     KLIPPDGIYAVFAHHNGIRYGGMLYIGSRPTLKTHHNRTIEVHVFDFKQSIYGQEVQL
                     ELVERIRDDQAFQDLDALRQQLQKDQEAAQAVLARHGWSKDSVQTQRKHPEAAIVILN
                     YNGRSHLERFLPSVLASGYPNMRVVAADNGSPDDSIAFLEKRYPEVEHLDLGRNFGFA
                     EGYNQALKTIPSPYLVLLNSDVEVTPGWLDPIIELMEHDRSIAAVQPKILSLNDKSRF
                     EYAGACGGWLDALGYPFCRGRIFAETELDEGQYDTVEDIFWASGA"
BASE COUNT         6467 a         7292 c         7476 g         6511 t
ORIGIN      
        1 gcgcaactga gccatctcaa tgaggttgcg ggcaatggct tcggcctgtc cgcgctcctc
       61 cgcttcgatg cccggataag cgcccggcgt atcaatgagc gtgatgacgg gaaagttgaa
      121 acgttcggcc agcttcatca ggcgcagggc tttgcggtag ccttcggggt tggccatgcc
      181 gaagttgcgg tattggcgca tcttggtatt gatgcccttc tgatggccga gtatgaccac
      241 cggctgcccg tcgagagagg ccagaccgcc cacaatggct ttgtcgtctt tgacacagcg
      301 gtcgccgtgc agttcgacga actttttgca catctggttg atgtagaaca gcgtataagg
      361 gcgctccggg tggcgggaaa gctgtacttt ctgccagccg ttgaggttgg aataaatttc
      421 cttgcgcttg tttcggattc tgctttccag ctctttgatc tgctccgtca catccaattc
      481 gccgtcctcg cctacctggc gcagtttgtc gagttgctcg tagagcgttt cgagcggttt
      541 ttcaaagtct aaaaaaacca tgagcggtta tttcaggtga gcggattcga cttctggaga
      601 tacaactcca gagccgtagc catagacggc gcttcggggg ccggcgcttt cacgttggcc
      661 accaaacttt tctcctccag cgctttgagg gtgctgttgc cgaacacggc aatgcgcgtt
      721 tcgttctgtt tgaaatcagg gaaattgtca aacagcgatt cgatgccttg cgggctgaag
      781 aacaccagca tgtcgtaggt gacgtcggac aggtcggaca ggtcgctgct caccgtgcga
      841 tacatgagcg cgtcctgata ttgaatgccc agttctttga ggtaagccgt cacgtcctgg
      901 ctgccgaggt tgttgcaggg cagcaggaat ttttcgttgc gatgtttttt gagcgccgaa
      961 gtgaggtctt cgatggattt gttgccggca aaaactttgc gcttgcggta gatgatgaac
     1021 ttctggaggt aattggcaac cgcctccgtg acacagaagt atttggtttc ctgcgacatc
     1081 ttgatgcgca tttcgtcgca aagccggaaa aagtgatcca cggcatattt actggtgaag
     1141 ataatcaccg tgtactcgtc ggggtaaacc cgttgcttgc gatactcttt agccgatacc
     1201 ggctcaacgt ggatgaacgg gcgccagtca atctggagtc cgtatttttt ttccaggtcg
     1261 tagtagggcg atctttctgg tttgggctga gaaactaaga tcgttttcac ctttttgtag
     1321 gtttcttcct tcgcttgaat cgttgcagac attcagttaa agatttgaag gtgaacccct
     1381 ccacagatgc ttcaagcctg attctgaatc agtttgacaa acaccaataa aggagcgatt
     1441 tcgacggcgc aaagatacag aaaaaagtgg aacttgtgag tggccaaaaa attgttggct
     1501 ataaggagac ctctcagcga acgaaatgcg tagagcaggc cggttatgcc aaacgtggca
     1561 aacagcgcgg gggtggtcat ttcggagggg ccataagcaa caaatacgtt ggcggctact
     1621 aaaaccaagc ttagcaaaat gccgaaaacc accatcgtaa attggtagtt gcttgtttct
     1681 ttgtccaccg gaaacaccgc gccgatatac gtcaaaacaa ggtgcttggc cagtaccaat
     1741 gccgccgttc ccgccacgca catcagccag ctttgccaca gcccccaggg gaaggtggcg
     1801 ccaaagtgcc gcgcaacgag gaacacaaag gtgcccgcat tgagtacaaa catgatatac
     1861 aaaatgtaat aggccaatgc gccggagctt tgcgcctcgc ggtgtatctg gttgagcatg
     1921 ttttcgttga gaaagccccg atatgctttt tgataaacgc ctctgaaaat ggtaatcagc
     1981 agcgtcagca gcgccagatt ggctacggta atggcaaaca gaaaggtttt gtattgctct
     2041 tgcgtggtaa gccgggggcg tggaggcttg gccgccggtt tggcttttgc tttgttgacc
     2101 ggttgcacgg cgcccgctgc gacagggttg accgcatgtg ccacgtcgaa agggttgccg
     2161 gtattccagg caggcggcgc cgtttcggaa ggggccagcc ggggcttcaa ctcgaagggg
     2221 ttcagcatgc cctgacccca tacgggagcg atcgttgcca gcgttaagac taaaatccag
     2281 gcaaatgctt tcaatggatt gattttaaaa attgctgcaa agatacaatt tccgttaatt
     2341 tgccgtcnnn nnnnnnnnnn nnnnnnnnng cctctgctat gaaaaacagt accatttttc
     2401 gagtcatcct gttgggcgtt gtggccattg ccggcatcat cgccatacaa tcctattggg
     2461 taatgagttc ctggaatctc aatgaagatg agtttcagaa aaaagtaaat ctggccctgt
     2521 acaacacggc ccgcagcttg gccgacctca acgggcatac cttgccggcc cggaacgtgg
     2581 tgcggcagcg cagcggcaat tattttgttg tgaatattgc ctacgagatc aacacagaag
     2641 acctggagtt ttttttacag cgggaactgg aggaactggc gctgaacatt gacttcgagt
     2701 atgccgtgtt cgattgtgaa accgatgaga tgatgtacgg agactacgtc agctactcgc
     2761 ccaaccaaaa ttcacaagcc aaaaccggtg aactgcccaa gcacagcgag tttacctatt
     2821 acttcggtgt tcgcttccct acgcgaacgg attatttgct gaaccgcatt cagttgtcca
     2881 tcttattttc ggtgattctg ttcgtggcgg tgatcttttt tgcctattcc atggcggtca
     2941 ttctgaagca aaaacaactt tcggaaaccc agaaagactt tatcaacaac atgacgcacg
     3001 agttcaaaac ccccatttct accatgggca tctctgcgga tgtgctgctc aattcgcctg
     3061 ctgtacaggc cgaaccccgc cttttgcaat acgccaaaat catcaaggaa caaaatcagc
     3121 ggctcaacca tcaggtggaa aaagtgctgc aattggcccg catcgaaaaa ggcaattttc
     3181 agatcaaacc cgaaaaaatg gagttggatg cttttctgga acaggccatt cagggcatgc
     3241 aaatacaggc cgagcaaaaa ggcggcgcca tctccggcct cttccacgca tccggctgca
     3301 tcattcaggc cgaccgtctg cacctgacca atgtcgtcaa caaccttctg gacaatgcgc
     3361 ttaaatactg tgccgataag ccgcaggtca tcgttaccac aatctgcaac aagggccgcg
     3421 taacgctttc catcaaagac aacggcatcg gcatcgccaa agagaacacc aaaaaaatct
     3481 ttcacaagtt ttatcgggta cccaccggca acgtccacga cgtcaaaggg tttggcctgg
     3541 gcttgtacta tgtgcgcaac atctgccagg cccatcgctg gcaactgcgc gtagaaagca
     3601 ctccgggcgc cggcaccgat tttcaaattt ccataccttt agcgccnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nngtggaaga cgacgaaagc ctgagcttcg tcacccgcga caacttagaa
     3721 atgaagggct accggatcac ccactgcgcc aacggccgcg aggcgctgga ggtattgcgc
     3781 aaccaaagct tcgacctctg tattctggat gtgatgttgc ccgaagtgga cggttttgat
     3841 attgcccgcg aaatccgccg cttagaccag cacacgccca tcatttttct gaccgccaaa
     3901 tcactgaaag aagatcgcat acacggactt aaaatcggcg ccgacgacta catcaccaag
     3961 cccttcagta ttgaagaact gattctgaaa atcgaggttt ttttgcggcg cagcaagggc
     4021 gctacttcct ccgcgccgac tttgtatcga atcggggagt atgaatttca tcctcaaaat
     4081 cttacgatag aacgcgccgg cgactcgcgc cgcctcaccc agcgcgaatc cgacctgctg
     4141 ttgatgttgg tgcagcaaaa aggccaggtg gtgaaacgct ctctgatttt ggaaaaactg
     4201 tgggggcaag acgactactt tctgggccgc agtttggatg tgtttatttc caggctccgg
     4261 cggtacctcg gcgctgatcc gaccatcaaa ttggaaaccg tgcatggcgt gggttttcaa
     4321 ttgctgaccg acatgtaaat gccctccgaa cgcggcgccc gaaaacgatg tgccgcgccc
     4381 gagtctggtt gttgttgaca tgaaaaaaac aaaaggcgca taccgtattt accttttgac
     4441 ggctctttcg gcggcgggct attttgcgtt gggccaaacg gggcgcacac atttttctac
     4501 gctgctggcc ctatacatcg tcctgtttgt cttgttcctt ttgctggtaa ggcaaatcaa
     4561 gatggcagat gctgtacggt tcggcatcgg ttgctcgctt gtacttcaat tagttttgct
     4621 gtttcatctg cccaatctct ctgacgatta tttccgtttc atctgggatg gccaactcat
     4681 tactcatggt ataaacccct actcagccct gccgccggag gtgctggatg tcattgcgcc
     4741 cacagcgccc atgtcggagg agctttttgc ccggctgaac gaactgcaaa gaagcaatta
     4801 cacctgctac ccgcccttca atgagttgtt ttttgtgttg ccggcctgga tattcccgca
     4861 aagcattttg gccaatgtag tgctgatgcg gctcttcatc atagcggcca atctgggtac
     4921 ggcgctgctg ggcctgcggg tgctgcgagc gctgggtcgg cccgaaagcg atgttttgta
     4981 cttcgcgctc aatccgctcg tggtggtcga gttggccggc aatttgcatt tcgaggcgct
     5041 gatggtcttt tttctcgtgg cggcgctcca ttttgccgtc aggaaacggc acttgtctgc
     5101 cggcgcggcg cttgcccttt cggcctctgt caaactggcg cctttgatgt taatccccct
     5161 tttttttcgt tttttcggct tccgcaaatg gacggcattt gctgctgtta cagtcggact
     5221 cacgctgctg ttgttcctgc cggtgccggt gttgggcggc gtggaggggt tttttaaaag
     5281 cattggactg tatttcaata actttgagtt caatgccggt atcttttatg gactgaaaca
     5341 attgggctac tggttcagcg gtcgcaatat gatcgccgtc attggtccgg ctttgtcggt
     5401 agtgactttg ggtattatct tttatctcac tttttttcgc aaaaacgaag ctcccggcac
     5461 actgatctcc tccctgcttt tagccatgac ggcgtattat ctattgggaa ctaccgtaca
     5521 tccctggtac atcgctacgc ctttggcact gtcggttttt acgtcctttc gctatccgat
     5581 ggtatggtct tttgcagtgt tctttagcta ttcggcctac gccgcaacgc ccttttccga
     5641 aaaccgttgg ctggtcggct tagagtacct gttcgttttg gccgtgctgc tgtgggaatg
     5701 gcggaagcga gaggagcctg ttcctgaacg cagatgaact tccatttttc attgcaaaac
     5761 aggtagttct ataaagcgct cgcggttcaa ggcctgcatg ttttcctcaa aaaacgcatc
     5821 acaatacagg caaacgtttt gcagaacctc cgttcgtttg cgcgcttctg cctcgctgat
     5881 gccgaggtgt tcgcccatgc gccagggatc gccctcgttg agttttttaa aaacaggatg
     5941 ctgcgatacg cgctccaata catcggctac gcgttcggtg cgggcgtcgc cgatagggaa
     6001 tttcgtgccg gggcagcagg gattgatgcg ccagagttga atgctgattt cctgcgggat
     6061 atcgtcgtag ccgcccagaa agttgcgggc gccggaaagg atggtacaga aattgtgctg
     6121 cgggtcgcgt ttccagatgc ctttttcgag cgccataccc ctggcccagt tgccgcccag
     6181 ccacatgtct tccgtagcgc cccaataacc ccaactcaac tcattttcaa tgagatagct
     6241 atccttgtct atgagggggt ctttgtgatc gccgcgcacg ccgtgggctt caaaaagctc
     6301 ggctaatact tccagtcttg ccccggcatg tttatggtag cggtcaatgc tggcgatgtc
     6361 gaagcgggag actccttttt cgatgagcgt ggcgaggatg ggttctgtga gcaggtcgcc
     6421 gttggtttgc aacatgatct gtgtgcgttc ggcgtagcgg tcccgcaggc ggtcgaggat
     6481 ggcgtagagc agtttgcgat ctgccaaagg ttcgcctccg gagaggatga ggcggtccac
     6541 ccggtcgggc atattttcta tgatggtcat gcactcctcc tcgctgatgc gggcgccttt
     6601 ggggctgctc cggttgtagc agtggtcgca ttgatcgttg caaagctgcg tgaacaccca
     6661 gtagaggctt tcgcaatgat tgaaatccgt tttcatcttg attggatatt tgtccaaaaa
     6721 cctatttcct cctgaacgcc ggtgcgggca tcctgcacga aatcggcttt ggtttcatat
     6781 ttttttaaaa ggccgtcctg catcagggct atttcgtccc ccatgagcag ggcctccttc
     6841 aggtcgtgcg tgacgaagat ggtcgtgatc tgactgccgg ccaccatgcg tttcagcagt
     6901 tgttgcatgg cagtgcgggt ttcggtgtcg agcgctccga acggctcgtc gagcagcagc
     6961 actttcggct ccacaatgag cgccctgccg aaggcgaccc gctgcctttg cccgccggaa
     7021 agttgtgcgg gcattttttt agcctgtcca ttgagttcga gatggtcgag catggtctcc
     7081 gttttttggc gaatgtcggc ctcagtccat ttgcgcagcc gcagcccgaa ggcgatgttt
     7141 tcaaaggcat tcaggtgcgg aaacaacagc ggctcctgat acagataaac gatgttgcgc
     7201 aggtgggcgg gcgtttcgtt catcgccttg ccctcgagtt ctacccggcc gttgtcgggc
     7261 gattccagtc cggcgatgat tttcagcagg gttgtcttgc cgctgccgga acgaccgaga
     7321 acgctcatca tgcggtgggc gtcgagcgcg aaactcaggt ttcggagtac ttgctccttg
     7381 ccgaaggttt tggatagatt gcttacctga agaaacatac tcacgaagtt ttgaacaaca
     7441 cctgtttatt cacccacaac aagcccgccg ggggcagtgc cagcaacaaa ctcgccagcg
     7501 ccgcatggaa aatattcgcc tcgccgatgt attgaaacac cttcaccgtc agtgtttgca
     7561 ccttgccgat gccgatgagc gtcgtcagcc cgtattcaaa ccaagagatg aggaaggcct
     7621 ggaaaaagca caccaacagc gccccctggg ctgccggtaa caacacccga aggaaggtct
     7681 gccgcttgtt tccgcccagc gtggccgata gctgctccat ggcccgcatc cggtcgttcc
     7741 agaaacccgt aaaaaaaatg accccgaacg gataggcaat caacaactgc gcgaacagca
     7801 cgccggggac attgcccgcc aggcccagcc ggaggaagaa gaattgcagg gtagcggcca
     7861 gaatgacggg ggcaaaaata tagggcaaat aagccagcgt cagccattgc tgccgccagg
     7921 gatggtacgc aatatgcttt gccgtgataa atcccagcca ggtagcggcc gtggcaatgc
     7981 ccgccgaaat gctcagggaa cgcagcagac tttgcgccag atcggattgc gaagtcagca
     8041 gcagttgcca gttgtccatc gtccacaggg caggaaacag cgccgggaag cgccagtttt
     8101 gcccaaaaga aaacaccagc agcagggcga agggaaacag caccgcgccg gcgaaggtgt
     8161 atttgagcat cgttttcagc atgttgatga cgttttttcg gcattgttgc gcagcaaaaa
     8221 taccgccgct gccgctacga acaccatgta caacactccg atggcatacg cctgcgggat
     8281 gtctgccaga tcgaaacgct ggagctttcg gatggtaagc acggatacca tctgcggcga
     8341 ctgactgccc aaaagcaggg gaatctcgta gctgcccatt acgaaaatga agtacagcac
     8401 cagcgtgggg aacgactgcc ggagcaacac cggcacgatg acccgccttg ccgattgccg
     8461 cgccgttgcg cccagcgtag ccgccaattg ggcatattcg ccgaggcgtt cgctttggta
     8521 aagctgggca aaaagaatga tgaaaaacgg cgtggccatg aatacatgcg cggcgatgat
     8581 gcctatgccc caggaatcat tcacccagtt gggaaagtcc tgaatgccgc tcgtcagtcc
     8641 cagcttatac gccagcctgg aaacaagacc tgctcccgac agcatctgaa acaccacaaa
     8701 ggccaccacc atggcgggca gcgtcagcgg caggtacatc agccaagaca ggccgccgcg
     8761 ttgccatcgc tccggtgcct tcagtacggc tgctagcgcc aatacccccg ccaggccgat
     8821 gctgcacccc gccacccgga aactgaggcc cagcgaacgc caaaactcgc cgccgctgag
     8881 caccgcctcc caatggcgca gcgtgaagcc ctctgctccc aagccggcca gccccatgct
     8941 gcgcagcaat gcgtagccga tgcccaaagc aatgggcagc gccgtgagcg caaggaacag
     9001 ccccagactt gcgtatttgc ctttatatat cccagccatg ttttgtccaa tcctcttcgt
     9061 aatcaatatc gggcagtacg ggcaacaggg cgcaggactt acccagccgg cccatcgcct
     9121 ccattgtttg tgaaaaaaca agcggcgtgc tccattctat gccggtgaaa aggctcggct
     9181 cgtactcgct cattcccagc aggtagtagc cgccgtcgtc tgtgccgccg atggcgaaat
     9241 cgtggccggc caattgttca aacgcctcct gaacgatacc ggaagtgatg cccggcacgt
     9301 cgctgcccac aatgactgcc cgtgggtgca gccgcaaaac ctcctcaaaa gcattgccca
     9361 tgcgctcgcc gaggtcgccg ttgcattgtg tttttttgat aaaaaggtca ttcggccact
     9421 cgtcgttttt ttcaataaag tcactgtaaa acaggtactt ggctacgggc atcggctccg
     9481 tcgtctgccg gatgcggtgg agcagggcac ggtaaatctg caaagccgtt tcatggccca
     9541 tcgtggcagc gaggcgggtt tttacctggc cgggaacggg atttttaacg aaaataatga
     9601 gggcgtcccg catggcttac cgttcgatga tttgctcgcg gaagtccttg gccagccgaa
     9661 tcatgtactc cggagcgggt tccttgagtg cgcgggattg catttcggca cgggatggag
     9721 cgtattttct ggcggcgaga ttttcaaagc gctgcttcca gtcgccgggc agcttgcttg
     9781 ggtcgagcac ggtgccgtcg ccccatgcct cctgtttttg tttttcccat tgggcctccg
     9841 gtgaaaggag gaaattggcc actatcatgg cgcccgcttt gttgcccgat agtttgggaa
     9901 tgcccatgta gtgcgagttc tggatgctgc cgaaatcggg cacataagcc cttgctgtgg
     9961 gcggaaacaa accctgcccc accttgctgt ccacctcgtt gtcgttgttg gtgagggtga
    10021 accagagttc gccgttggaa aaaagctggt gcatggggga gcgggaatcc gggaaggtct
    10081 tgccctgttt ccagagataa ggttgtatgt cccggaggta gtcccagagt tgagcgctgt
    10141 attgcccgta tttcgcctcg tcgaatgggc cgtcgagggc attgccgccg ccggcaatgt
    10201 caatgagcag cgacttgagg aaggtcagtc cggtgaactg cgtatcgaag gtgaactttc
    10261 cggggtgtgc tttcacaaag tctgccagtt gcgcccgggt gcggggcggg tctttcacga
    10321 actcgctgtt gtaaatcagc gtcatctgca cgttgcccca gggacattcg tagccgttca
    10381 cgggctgctg gaaatcggta ttgataaaag gattttgaaa atcaatgaat tgagcattgg
    10441 gcagccgctc ggtccagggg ccgaacaggg cgtcaatctg gcgaagctga taaaaggtct
    10501 cgccgttgat ccacatcagg tcgagttcac cgacagattt cccggcttcc atttcgnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn cgtgatgctg aactgctgct tcactgccgg ggccacatac tgctgcatgt
    10681 aggcgttgat gagcgggtcg ccctgccaca tcatcagttg gacggtgctg cctttggctt
    10741 gttcgatcac ttgctcccag gggaggcggg taggatcggc ttcgtggctt ggctgcggat
    10801 tgttgcaggc tgtcagaaag aggtgcgcaa gaaggaggaa tcgctgcatg atgagtaaaa
    10861 aattaaaccc agaaatcgag cttggcaggt agctgcaccg caggctgcaa gcatcatcgg
    10921 ccccaaatat cgcattttaa aacaaccacc tggccccaaa ctggaattgc ctcggcgctc
    10981 cggcgttttt ctccacgatg ccccgcgctc tgccgcccac ctgaatctgg ttgctttggg
    11041 tggcgttgtt gctgtacccg ctcaggttga cagtattgag gatattgaaa acatcggcgc
    11101 gcaattccaa ttggtgtttg cccaccggca gcttgtattg caaacccaga tcgaacacct
    11161 tcgaccaggg caggcggtcg ctgttgcggc tttcgccggg atggcggtcg ctgttgccca
    11221 catacgcatc accgaaggag gcgccgtcgc cgttgaggtc ggtagtgccg tagagcagtg
    11281 cgtcggggat gcgattgacg ggctgcccgc tttgcaccaa accggccagc gtaacgccca
    11341 gcccgcctgt tggaaaccaa ctgaggatgc cgttgaaaat atggcgccgg tcgttgatgc
    11401 ttggccccca ctcggcctca aagtcattgc cgtccatggc gcggaagttg atgtcctcgg
    11461 tgttgttttc cagatacgac aaagtgtaat tgatgcgcca tgcgagtttg ccatcgcccc
    11521 ggtcctgttg caaattgaaa cttgcgccat aatacctgct ctcgccttcg gtttcggaca
    11581 tcaccacatt gcgagccacg ccgaaaacct ggctgccnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggcg ggagtgcgca
    11701 ccgttacatt atccgggttg gcaatggggt aggctgctgc ggcgttgagg ttgcgcaggc
    11761 ggaagagatt ttgggagcgg ttgtgtacca agtccacata aaacagccgt ttttcattcc
    11821 attgcaactg atagcccagc gccatttgcc gggtgagcgg gttgtcgtag ccattgggat
    11881 tgaggatgcg gcgttcgttg ctgaacacgc cctcccgctg cccttgcaga ctcccgaaac
    11941 caggcccttt caggtactcc acgttggccg tgctgccgct caggttgccg tcgaaggtga
    12001 tgcggccgct ggtgggcggc tgcacgcctg acaggatgtt gagcagcgtc gacttgccgg
    12061 cgccgttctc gcccatcagg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnctc gaccgggccg ctatccggcc tcttgggggt gaagttgaaa
    12181 cgaggcgcga ggttgttcag gtcgccttgc tcgctgccgc ctttggagag gttgtcgtag
    12241 tcgtagcgca gccccagcgt gaggttgagg cgatcagaga gggtaaactg gtcttcgaga
    12301 taaagggaca aaatattttg cgttttgccg aaagaggccg gtcgcagttc cacattgtaa
    12361 ttcaaaacct gcacatcggc gggaatgtcg ttcacattga gattgctgcc cagggcagat
    12421 tcccggagtt gcgagagttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnaatt tttgcaacaa ctgcaccgtg ttttccgtgg cgtcgaaaat ataacccgga
    12601 tgccccagca cggctacagc ctgttcggac ggatcaagca cgacgacttg tgggctgcct
    12661 tcgttggccg ctctgccgta attccagcgg aagcgggcgt actgaagatt ggtttctgaa
    12721 ctgaaacgac cgctcacata agtatttttg gaggccagca gcagggaatt tctatcctga
    12781 aaattgccgg aggagggaaa tcccacgccg ccttccaaac cgccgccctg tcgctctatg
    12841 gctaccatgc ccacattggc ccggatggag gagcgaaatt tggaactcca aatctggtct
    12901 attttacccg acaggtaggt gaagcggttt ttgccgcgca ccgtctcgtt cacgccgagg
    12961 gcaggcgagt tcaggaggtt gtctttcagg tcggtggtat gctccagatt gaggtagtaa
    13021 aaggtcttgt ctttacggat ggcgccgccg aatcccaggc cggcctgata gcgctggaag
    13081 ccgtctttga caaaattgcc cgacaggtct cgctgtgcaa agggcgagcg cgcatcgagg
    13141 gccgggccgg gccggttgac gatgaatgcc tcgccgctga actcattgcc accggagcgg
    13201 gtggtgatgt tgacgacgcc gttgttggtc aggccgtatt cggcagagta gttgtttgtc
    13261 agtacggtga tgtctttggc aaacccgacg ggcatggcaa atttctggcc gccgaggaag
    13321 cgttcattat tgtctaaccc gtcaatcaag tagctggtaa acaaaggatt ggcgccgttg
    13381 atgctcacgt tgggcgcttc ggggtagaag ccggtagcct gcgaaacatt gggcaggcga
    13441 tacagcgctc gtgtgatgtc tcgtccttca atgggcagtg cttcgatttc ttttttggaa
    13501 agctcgaagg agacctccgc atcggtgcgg ttgattttgg cggaggatgc cgccctgacg
    13561 gtcacttctc ccagagatac cgttgtttca gagagtacca tctgcacggt gcggttttcg
    13621 ttggagcgca ggttgatgag gtcggattgg gcggcctgat aaggcaaaaa atcaggtatc
    13681 agtaccctgt agccgtccac cacgggcagc gagcgaaaaa cggcccggcc ctgagcgttg
    13741 gtttctgtgg tttgttcaat cccccatgta gcatttttca gttgaaccgg agcattggct
    13801 accggccggt tttgagggtc gaggacggaa acggtgaggt cttcctgagc gaggagtgtt
    13861 gtgacaaaga gtgtcaggat catccataaa aaaagtcgca taaaacgttg tttttgagtt
    13921 ttgtaaggaa aataaaaaca caggccggga gacaaaaacc ggtgcgtctg ttattgcaaa
    13981 cactgcgtac ttggtggcga agaaaagtaa aacctgacag gaatcgcgtg aaatttttgt
    14041 aataatttga tcggccggag agcgtatcgt cgccggcgtg cggccggagc atttcgtgat
    14101 cgcagccgag gggtggccgg ccacccatta ccggcaacgg atgcgtggca ctatgggatg
    14161 ttgcggactc aatataaatt tacacatggt cgttttttta tcttcaaatc acgaaaaatg
    14221 ttcaaaacac tttcttttct ggtcgttttc atggtggctt ccgccaatct ggccgctcaa
    14281 aagccggtgc aacacgatat ctgggacgcg ctgctcaaaa aacatgtgcg caaagacggt
    14341 tttgtggact acaagggctt tgtgaaagac agcgcccggc tcaaccagta tttggaggtc
    14401 ttgtcgtcgg cgcacccgca atcatcctgg tcgagggccg agcaaatggc gtactggatc
    14461 aatgcttaca atgcgttcac tgtcaagctc atcgtgcaac actatccggt ggcaagcatc
    14521 aaagacatca aaaaaggcat cccattggtg aatagtgtgt gggatattaa gttcatacac
    14581 attcaggaca aaacctacga cctgaacaac attgagcacg acatcctgcg cccgcagttc
    14641 aaggacgccc gctgccacgc cgccatcaac tgcgcttctt attcctgccc gcgcctgcac
    14701 aacgaagcct tcactgccgc aaaattggag cagcaattgg aggccgtcac ccgcagtttt
    14761 gtgaatgatc cgctgcgcaa cagaatcaag gccgacagag ccgagctttc cgctattttc
    14821 aattggttcg gcggcgattt caaacgagac gcaggctccg tgagagcgtt tgtcaacaag
    14881 tatgcccaag tcaaggttaa agacacgggc aaaatcactt ttatagacta tgactggagc
    14941 ctcaacgacg tcaaataatc caacacaata atgcccggca gcaagacgat gaggtctcga
    15001 acgcatccct gctgtctgat gaactcgcca gacgattgga acggcggatt ctgaaaaaat
    15061 cagatgggca ggaagccccc tcacaaggga agtaattttg tattgaataa gtatgaaccc
    15121 gaagcatacc agtgctaaag cattaacttt tgaaaagacc gtgaaccaag ctaaatgatc
    15181 gaaaaaccta tgtaagtaac aaggattatt catgtatttg gaaggggaaa attgacctta
    15241 aagccataac ttattcaata ttttttcgcc acagttgatt tgttgctttc gcgcaagctg
    15301 attacttttg tagccgaaag aaaggaaaag tcaaaatcca cagttggatc aagaattaca
    15361 agattgtttt catttggttt tttggggtta tacaagaggc aattcaataa ggattgcctc
    15421 ctttttttgt gcaggcgccg agaaacaaat aaaaactccg cgacaataca acaatccgaa
    15481 gaatttccgt tctttgcgct ggaaatgagc gaaggtcttg cttttgcctc atcagaaaca
    15541 cattttgcac gggaaacaaa cccggaagca ggtatttaaa acagggaaga accttccatg
    15601 ggattattca gcttttttcg ccaggaattg gcgattgact tagggacagc caatacgctc
    15661 atcattcagg atgacaaagt agtggtggac gaaccgtcca tcgtagctat agacagaaaa
    15721 accaactcga ccatcgcagt gggtaaacgg gctatgcaaa tgcacgaaaa aacccacgag
    15781 gacatcaaaa ccgttcgccc gctgaaagat ggcgtcattg ccgattttca ggctgccgaa
    15841 agcatgatcg aaggcttgat cggtatgatc ggcaagcggc gccgattttt ctcgcacctc
    15901 aaaatggtca tctgcatccc ctccggcatc accgaagtgg aaaaacgcgc agtgttcgat
    15961 tccgcagacc atgtggactc taaagaaact tacctcatcc atgagcctat ggccgccgcg
    16021 ctgggcattg gcctcaatgt ggaagaaccg gtaggcaaca tgattattga cattgggggc
    16081 ggcaccactg aaattgcagt cattgcactt tccggcatcg tctgcgacca gtccatccgc
    16141 attgccggcg acgaactcac caacgacatt gtgagctaca tgaagcgcca gcacaatatt
    16201 ctcatcggtg agcgcactgc cgagcaaatc aaaatcaacg tcggttcggc gctgcctgaa
    16261 ttggaagacg ggccggaaga ttatgccgtg aatggccgcg acctgatgac tggtatcccc
    16321 aagcaaataa aagtagggta cgccgaaatt gctcattgtt tggacaagtc tattgccaaa
    16381 attgaagatg ccgtactcaa ggcattggaa ctgacgccac ccgaacttgc cgccgatatt
    16441 tatctcacgg gtttgtatct cacggggggc ggggccttgt tgcgcggttt ggacaagcgg
    16501 ctttctgcca aaaccaaact caatgtacac attcccgaag acnnnnnnnn nnnnnnnnag
    16561 tagtgcgcgg cacgggaatt gccttgaaaa atacagaccg attcgccttc ctcatagaca
    16621 ggaggagcgt ctgatgcagc cctctggcgc atcggcctcc cggataaact gcaaacgcta
    16681 tgcgcaacct gctcgaattt ctcgccaggt ttggtggttt ttttctgttt ctgatactgg
    16741 aagcactcag tttttttttg atcgttcgtt ttgacgacga taaaaacaga attttcctca
    16801 gttcttccaa tgctgtggcc ggctatatgc tcaaaaagta tgatgccgtg gcagactatt
    16861 tttcccttcc cggggaattg gatcagttgc aagaagaaaa caaacgactg agggagcagt
    16921 tggccgagtc ttattacgct cctgattacg atgtggatac ggtttcgctt acggtcagaa
    16981 cacaggattc tatttactct aaaggcgtag ttgtgcgaga caccctgcaa aaacaggatt
    17041 ctatggctac tcagatatat gcctacgtag aggccaacgt catttccaac tccatcaacc
    17101 gccgcaacaa caccctcacc atagaccggg gcacatcgca ggggatagcg ccaagaatgg
    17161 gagtaatcgg gccgcagggc atcgccggca tcgtcaaaac ggtgtcgaaa cattacgcat
    17221 cggtagtttc tgttttacat tccgacatca gcgtatcggc taccgtaaag cataaaggct
    17281 acttcggcgc tttggtatgg aacggccctg atcccagata catgcaactg gaggccattc
    17341 ccaaacatgc cgaaattgcc gccggagata ccatcgtaac gagcagtttt tcaggtgtgt
    17401 ttcctgccgg ccttctgatt ggcatcgtag agtcggtcac tcagaatccc ggagataatt
    17461 tccacaacat caaggtagcg ctggcttccg atatgagtcg tacgggacgc gtatatgttg
    17521 ttcagaataa atggctggaa gaatataagc aggtacaaaa acaagaggag gacaggaaat
    17581 gagcggctta gtcattacca acggcattcg gtttttgata cttctgatcg ttcaggtatt
    17641 ggtactcaaa cgattgtcgc cgggcataga gtctttcaac tacattcata tcttattata
    17701 tccgcttttt atccttttgt tgccgctgcg cacgccgcag gcgttgacac tggggctatc
    17761 gtttatgctg ggcctgatgg tggatgtttt ttacgattcg ccgggggttc atgccgctgc
    17821 cagtgttttt acgggctatc tgcggggcct tgtgctgggg tacatggaac ctcgcggagg
    17881 gtacaatgtc aacttcagcc cgacgaaaga gcgcatgggg tttcgatggt ttgctggata
    17941 tgccgccgtg ctcatggccg ggcatctttt cttttatttt tctatggaag cctttacatt
    18001 tgcgtacttt gcagatactt tgctgaaaac gctgttcagt ttcgtggttt ccatgggatt
    18061 tgtgcttgcg gtgatgtttg tttttaatcc ggagagttag ccaaaatgtc agatttattc
    18121 aaagggcggc aacgggtcat acaggccatt tttatactgg cggccattgc gctggtaggg
    18181 aaagccatgc acttacagct ttttgatgag gaatttcgca aaaaggcagg tgcggcggct
    18241 gtggtcagag cgcctcaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnccccat ttatgacctg atggtgacct actcccagat
    18361 caagccggga atggacactt cccatttttg ccaactgctg ggcatcacgc cggaatattt
    18421 caaagagaat ctgacaaaag actggcgcag cgggcggttt tccaaaaacg taccctttgt
    18481 ttttctcagc aaagtcagcg cagagactta cgcccgcttt caggaaaata tgttcgattt
    18541 ccctggtttt cagatggttt tgagaagcat tcggggatat ccacaccaaa atgccgccaa
    18601 tgtgttgggc tatattcgag aagtggacag ccgcgactta gaaaaatacc caggcttgta
    18661 tgctcccggc gattacatcg gcgcgtcggg cctggaagca tcgtatgaaa ccctgctgcg
    18721 cggcgccaag ggcgtcagtt acatcctcaa agacaatctg gggcgcaaca tggggccgta
    18781 caaagatggc cagttggact ccgcagcggt atccggcgcc gacctgatta cttcattgga
    18841 cattgatttg caggcttatg gcgagttgtt gatgcaaaac aaaatcggcg gtatcattgc
    18901 catagaaccc tctaccggcg aaattctggc gatggtcacc tcggtgtctt acgatcccaa
    18961 tttgctcacc atcacacaaa agcgcggcgt ggcatttgcc gccctcaacc gcgacccgct
    19021 caagccgttg tttgacagaa cgacgatggc tcagtatccg cccggctccg ttttcaaatc
    19081 cattctaggt cttattgcta cccaggaagg cgtgtggaac atcaatcgag gcgtaggttg
    19141 tggcggaggg tatgtcttaa acgggcagcg attggtaggt tgccacggcc atccatattg
    19201 cagcaatctg gagatggcta tccagcattc ctgcaacgct tattttgtaa ccgtttaccg
    19261 ggaactgatt gataaatacg gcttctacaa tcccgctaaa ggattggacg agctgaacga
    19321 atacttggtt cgctttggtt tgggcgcacc cttaggcatt gatctgcccg gcgaacgtgg
    19381 cggctattat cccaccacag cctattacga caagacctac gccaacagcg ggggcaagtg
    19441 gaattccgtc tggattcggt ccaacggcat cggacagggc gaacacctga tgaccaacgt
    19501 gcaaatggcc aatctggcgg cgattattgc caataaaggc tattacatca cgccgcattt
    19561 gctgaaagga tttaaaagcg gaggcagcga gatacctgaa ttgtaccgcg aaaagaaaag
    19621 cgtcggcata gacgcgcaac attttccccc ggttatcaac ggtatggaaa aagtgactat
    19681 ttcgggtacg gcgcgctcgg ccttcattcc gcacattccc atttgcggaa aaacaggaac
    19741 tgccgaaaac ccgcatggca aagaccactc tgtatttttt gggtttgccc ccaaagaaaa
    19801 tcccaagatt gcctttgccg tgtatgtaga aaactcgggc ttcgggggca cttacgcagc
    19861 gcccatcgcc agcctgatgg tggaaaaata cctgaccgac acaatcagcg cgccgcgcaa
    19921 atttttagaa aaacgaatgc tcgatgccaa tttgctccaa ttccagaaaa ccaagcctta
    19981 acgcgctctg gcttttcatt gcggttgcgt tcccgctctt tttacaggct caatcagaca
    20041 atgtacgccg cctcaaactc atttttgccg gcgacatcat ggggcacgaa actcaaattc
    20101 gcgccgccga ggtggaaaaa gacaaattgt acgactatac gccctgcttc gagtatgtgg
    20161 cgcccatcct gaaacaagcc gatctggcca tcggcaacct cgaactcacg ctgcccggca
    20221 aacctcctta caccggctat ccgatgttca aaagcccgga agaactggcc ctggcgctcc
    20281 gccatgccgg tttcgacctg ttggtcactt ccaataacca ctccaacgac ggcggcctga
    20341 acggggtcat caacacactc aacaccttag ataattacaa cttttaccac accggcacct
    20401 tccgggattc gctggagcgg aacgacttct atccgctcat tgtgtataaa aacgatttca
    20461 ggctcgcttt cctcaattac acttacggca ccaacggact gattaccaag ccgccctcgg
    20521 tggtcaacct tattgacgag caactcatcg ccgccgatat ggaaaccgcc cggcaaatgg
    20581 ctcccgattt catcatcgtc atcatgcact ggggtgacga ataccagatc atcgaaaaca
    20641 aaacccaacg cgatctggcc gccaaactca tcggctgggg cgccgacctt gtcattggag
    20701 cgcacccgca cgtcgtgcaa cctgtgaggc aggaaacagc cgcaggcgcc gacagtaccg
    20761 gacgcaaagt gtggacggct tattcgttgg gcaattacat ctccggtcag cgcaaaatca
    20821 acaccgacgg cggtatgatg ctggaggtag agttgcaaaa aacactgccc gccggcccca
    20881 ctgcgctcac cgacatcgcc tttattccca cctgggtctg gattcaaacc ctgcccaccg
    20941 gcaaaaggca ctatcgcatc ctgcccgttg cagcgtttga acaggaaann nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagttt tttaacgcga attttggcga
    21181 agggcgcgaa tttccgcgaa tgcgagggac tttgtggctt acggctaaaa gccggcagcc
    21241 gttccaacct gcaaaggctc gcggcttatg gcttgcatct gattttttct ctaattttac
    21301 tgtccaaaaa aaacaactat gaaagcaact cgtatcctct ggttactcat ggccatctcc
    21361 attattgcct acccctcctg caaaaccaac cgaaccgtga aaggcgccgt gattggcggc
    21421 acggtgggcg gcgtggcagg cggcgccatc ggtaagaagt cgggcaatac ggcggtgggc
    21481 atcatcattg gggcagctat cggcggtacg gccggcgcac tcatcggcaa ttacatggat
    21541 aagcaagccg cagaaattcg ccgggatatg aaaggcgcca aagtagagcg cgtcggggag
    21601 ggcatcctca ttaccttcga ttcggggctg ctgtttgatg tcaattctta tgaactgcgc
    21661 agcactaccc gccagaattt gaacgaattg gccaaggtgc tgggtaaata cgacgatacc
    21721 gacatcgtga tacaaggcca tacggacaat acaggcggca cggaactcaa ccagacgctt
    21781 tccgaaaacc gcgcccgctc ggtgaagagt tacctcatgc agcagggcat cgtcatttcc
    21841 agaatgtacg acatgggcat gggcgaaaat tatccggtgc aggacaatgc cacagcggcc
    21901 ggcaaacagg ccaatcgccg cgtggaagtg gctatttatg ccaataaaaa gctgaaaaaa
    21961 gccgccaaac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggaaattgaa ccgtgaacca
    22021 aatcaggaac tttgaggtca tgggagcaaa ccttcgcaga tgcaggcata cgagattaaa
    22081 ttattggaaa cggcgctgat tcttggtgcg tttttggtcg ccagattgat tttgtcgaag
    22141 ttcttccttc gggtcaaccg catatttcct gcgcagaaaa acaggattga cggcttcaga
    22201 aaaatcctga acctgatact gggcgcagtg agtacggtgc tggtctttat tgtgtggggc
    22261 gttgaccaaa aggaattgct cctgttcgtt tcttcgtttc tgacggtgct cggcatcgct
    22321 ttttttgcgc aatggtcctt gctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnaaagg gggcgatacc attatgattt tggacaagga cttttctgct cagggcctca
    22441 ttcgggaaat aggcattttt tttattcaca tcgaaacgac gccgggcgaa acgatcacgg
    22501 tacccacctc gctgtttttg caaaaaatgg taaaaattgt ggagcagtcg gatgcttcga
    22561 aagcaagtag caaggaatga cttcaaccac tttcaccgac ctactcagcc gcctcattgc
    22621 cacgccttcc ttcagcaggg aagaacacgt tacggctgaa ttgctggcgg attttattca
    22681 caatcaggga attgcctttc ggcgaaagca caacaacatc tgggcggcca atcttcattt
    22741 cgacccggcc aaacccacca ttttgctcaa ttctcaccac gatacggtca agcccaattc
    22801 ggcctacacc ctcgatccgt ttgaaccgct tgtgcgcgac ggcaagctgt acggattagg
    22861 cagtaatgac gccggggccg cgctggtatc gctgttggcg gcatttctgc acttttacaa
    22921 gcgggaaaat ctgacccaca atctcctctt tgccgccacc gccgaagaag aaatttccgg
    22981 tctcaacgga gtggagagca tcctgcctga attgggcgac atcgcgctgg ccatcgtggg
    23041 cgaacccacg ctttttcaga tggcggtggc cgaaaaaggc ttgctggtgc tggattgtac
    23101 tgcacaggga aaggccgggc acgctgcccg cgaagaaggt gtcaacgcca tttatgaagc
    23161 catgtccgac atagagtggt ttcgcagcta ccgttttccg aaagtctcgc ccacgctcgg
    23221 ccccgtcaaa atgagcgtca ccgtcatcca ggccggcacg cagcacaatg tggtacccgc
    23281 cgaatgtcgc ttcacggtgg acgtgcgctg caccgacgcc tactcgcacg aggaattgtt
    23341 agacatcatt cgcagccagg tccgggcgga agtcgtgccc cgttctatgc gattgcgccc
    23401 ctcggccata ccgatggagc accccattgt gcaggccgga ttggttatgg ggctgagtgc
    23461 ttacggttcg cccaccagtt cggaccaggc cctgatgccg tttaccagca tcaaaatagg
    23521 gccgggcgat tcggccagat cgcacagcgc cgatgaattt gtttatttgc acgaaataga
    23581 acagggcatt gccggctaca tccggctctt ggaacaggta ttataatgtg ctattcagta
    23641 tatctcttaa agctaatggc ttggggctaa cagcttgcag cttttgggat gaccctaaat
    23701 aattaaggat gatgaaactt tggcaaaaag aaggaaacga ggccggcgaa tggagccgca
    23761 ttgtggaaac attcaccgtg ggccgcgatc tggagttcga cattttactg gccgaacatg
    23821 acgtactcgg ctcgctggcg catacgcaaa tgctgcaaag catcggcctg ctgactgcgg
    23881 aagaactggc aagcatccat tcggggctga acgccatcct cgaagacata cgcgccgggc
    23941 ggttttcgct ggagccgggc gtggaagacg tacactcgca ggtggaactg caactcaccc
    24001 gcagcatcgg cgaaaccggc aaaaaaattc acagcggccg ctcccgaaac gatcaggttt
    24061 tgctcgacat caagctcttt ttgagggcat cgctgcggca aacggctgcg cacattcagg
    24121 cgcttttcga gcggatgcaa tcgctgagcg aagcgcaccg agagcggctg ctaccgggct
    24181 acacgcattt tcagatcgcc atgccctcct cgttcgggct gtggttcggg gcttatgccg
    24241 agagcctgtg cgacgacatg gagctgctgc tggcggcctt tcgcattgcc gacaaaaatc
    24301 cgctgggttc gggcgcgggg tatggttcct cctttccgct tcagcgaaaa atgacgacgg
    24361 aactgctcgg cttttccgac ctcaattaca atgtagtgta tgcccagatg acgcgcggca
    24421 aagctgaaaa agcagtggcg atggccctgt cttcggtggc ggctaccttg tccaaattgg
    24481 cctacgatgt atgcctgtac atgaaccaaa acttcgggtt tatcactttt cccgatgcgc
    24541 tcaccacggg ttccagcatc atgccgcaca aaaaaaatcc cgacgtattt gaactcgtgc
    24601 gcgccaagtg caaccgcata caggccctgc caaacgaact cacgctgctg accaacaacc
    24661 tgccctccgg ctatcaccgc gacctgcaac tgagcaagga aattctcttt cccgccatcg
    24721 ccgaaatgga caattgtttg gaaatgacgg agttcatgct ccgccatatc atcgtgaaag
    24781 acggtattct cgacgatgaa aaatacctgc acctcttcag cgtggaggcg gtcaacgagt
    24841 tggtgctggc cggcctgccc ttccgcgatg cgtaccggca agtggggcaa gccattgaaa
    24901 aaggcgaatt ccacgctccg gcacaactga atcataccca tgagggcagc atcgggcggc
    24961 tgtgcaatga gcagataagg gtgaggatga agggtttgat gagaagtttg gggtgaggat
    25021 ttctgttcag cgccggcggg agatgggagg ataatgagat tcaggatgac gcaacagcag
    25081 aaccagcttt taggaagtat ttggtaggag cgggtaaaac gttagaccat gatgttaaaa
    25141 attaagcttt tattactcct gtcaatggtg gcactctttg cttgtattga tagaggaaag
    25201 gacatagcgg ataagaattt atctgcgcca tttccaccaa tagaccacta ctatttaacg
    25261 ggtcagcaac ctttttcggg ggaggacttg gtgtcacggg gcaggataac gggttttcac
    25321 aaggcgacag ggttggtcca ctcctacacc ttggtgtacg atatcatcaa gcgttcatac
    25381 tcattaacac attatggaac cgcagaggat gggaaatatt taaaaaggcc aatagatttt
    25441 atactggcag attccatact gacaaatatc aacgaggcat ttgttcatgg cacgattaaa
    25501 agaggtgaca catatttgga cacgccacat tctgattatt tgtcattgac aatagaggtt
    25561 tcaataggag aagccgagcc tatgtcggca gagtattttt ttttacgatt ttgatgacct
    25621 ccctaacgaa gccaaatatt ttgcgcgtca atatttgacc gacagcttat ggatacctgc
    25681 ccaattgagg cgttaatgaa tcaggttgac tgctcaaaat cacttggcag cattgaggaa
    25741 aagcgtattt ttgccgggca acaatacatt ttcttaacca aaataacacc aagtcatgat
    25801 tataggagtt ccgaaggaga ttaaggacaa cgaaaaccgc gtagcgctga cgccggcggg
    25861 tactcgtgaa ctgaccaaac gcggccatac cgtatatgtg caaaccaatg ccggcttcca
    25921 aagcggcttc cccgatgcgg aatacacgca ggccggagcc gtcattttgc ccactgcgga
    25981 ggaggttttc caaaaggccg agatgattat gaaagtgaag gagccgattg cgcaggagta
    26041 tccgctgatt cgccccaatc aattggtttt cacttatttc cactttgcgt cttatgaacc
    26101 gctcaccaaa gccatgattc aaagcggcgc agtgtgtctg gcttatgaaa ccgtggaact
    26161 gcccgaccgc tctctgcctc tgctcgttcc catgtcggaa gtagccgggc gcatggccat
    26221 tcaggaaggc gccaaatatt tggaaaaacc gcagaaaggt cgtggtgtat tgctcggcgg
    26281 cgtacccggc gtagcacccg gcaaagtgtt ggtactcggc ggcggcattg taggcacaca
    26341 gtcggccaaa atggatgcag gcctcggtgc acaggtcacc attctcgatg tgagcctgcc
    26401 gcccatgcgc tacctctccg atgtgatgcc cgccaatgtg atcaccatgt attccaacga
    26461 gtacaacatt cgccgcctca tccgcgacca tgacctcatt gtgggcgcca tcctcatacc
    26521 gggcgccaaa gctcccaagc tcatcacccg cgatatgctc aaagaaatgc gccccggcac
    26581 cgtgatggtg gacgtggcca ttgaccaagg cggcgctttc gaaaccagca agcccaccac
    26641 ccacgccgat ccggtgtata ttatagacga cgtggtacac tattgcgtgg ccaacatgcc
    26701 gggcgcagtg ccttatacct ctaccattgc gctcaccaac gccaccttgc cctatgccat
    26761 cgaactggcc ggaaagggct ggcagaaggc ttgcagcgac aatgcagccc tcaagctcgg
    26821 cctgaacgta gtgggcggaa aagttgttta ccaaggggtg gccgaagcct tcgatttgcc
    26881 ctacactccg gtggaggagg taatgtaata cagacttatt tgcggtgcta tgagagtatt
    26941 ttttgaactg aatgccctgc cggagtttcg gaatgcggtg ctaaccatcg gttctttcga
    27001 cggcgttcat gcagggcatc aggaaatcct gcgccgcgtt attcgtttgg cccgccaaca
    27061 caagggcgag agcattgtag tgactttcca tccgcatccg cgagaggtgg tctatcctca
    27121 ggacgaccgc cccttgcggc tgctcactac catccgcgag cgcatcgaat tgctggaaca
    27181 atacggcata gacaatgtgg tggtggtgcc gtttaccgtc gagttttcgc aacaaagcgc
    27241 cgacgagtac attcaaaaat ttctgatcga atccttccga cccaagttca tcgtcattgg
    27301 ttacgaccat cgtttcggcc tcaaccggca gggcgacatc aacttcctgc gctggcacgg
    27361 cgaacagggc ggctacgagg tggtggaaat tgaacaacag atcatagaca acaatgccgt
    27421 cagctcttcc aaaatcagag cggcagtaga gcgaggcgat atgcctgtcg cccgcaccct
    27481 gatggagcat ttttttcagc tttcggggcc ggtagtgcgg gggcagaaca tcggaatgga
    27541 actaggattt cctaccgcca acctcgatat tccggccaaa aacaagctca ttccgcccga
    27601 cgggatttat gcggtttttg cccatcacaa cggcatccgc tacggcggga tgctctacat
    27661 cggcagccga cctacgctca aaacgcacca caatcgcacc attgaggtgc atgttttcga
    27721 tttcaagcag agcatttacg gacaagaggt gcaattggaa ttggtagagc gcatccgcga
    27781 cgatcaggca tttcaggatt tggacgcctt gcgacaacaa ttgcaaaaag atcaggaggc
    27841 ggcacaggct gttctggctc gtcatggatg gtctaaagac tccgtacaaa cccaacgcaa
    27901 gcaccccgaa gcagccatcg tcattttaaa ctacaacgga cgttcgcatt tagagcgctt
    27961 tctcccctcg gtgctggctt ccggttatcc caatatgcgg gtggtggcag ccgataatgg
    28021 ctcgcccgac gattcgatag cctttttgga aaagcgctac ccggaagtgg aacatctgga
    28081 cctggggcgc aatttcggtt ttgccgaagg ttacaaccaa gcgctcaaaa ccattccatc
    28141 gccgtacctc gtcctgctca attccgatgt agaagtgacg cccggctggc ttgatcccat
    28201 catagaactc atggagcatg atcggagcat tgccgccgtg caacctaaaa tattgtccct
    28261 gaacgacaaa agccgctttg agtacgccgg ggcctgcggc ggctggctcg atgcgctggg
    28321 gtatccgttt tgccgggggc gcatttttgc cgaaacagag ctggatgaag gccagtacga
    28381 cacagtggaa gacatttttt gggccagcgg cgc
//
LOCUS       DAORLF010000009        27412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_965_length_27412_cov_2.155280, whole genome shotgun sequence.
ACCESSION   DAORLF010000009 DAORLF010000000
VERSION     DAORLF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27412
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_27412_cov_2.155280"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>867)
                     /locus_tag="PK198_01770"
     CDS             complement(<1..>867)
                     /locus_tag="PK198_01770"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37488.1"
                     /translation="TTPIFTGSSFPLSFLTPGTYSYTAIAEDANGCRDTASVTINLAN
                     FQPGPLTSPQTICANTATLLNPGGNPAYNYTWSPTTGLDLSNPSNPIAVLNASTTYNV
                     TVTDPASGCSVQQQMVVNVFPLINLEARPDTVLCEIAPVTLTATASVNVSYSWFLNAF
                     NNPVGSGASITVTPAKGSNNYIVVAQDTNGCADTTSVNVTVVEINTGLPDSIRVCIDV
                     PTPINPEGNPSYTYTWSPTTGLNLSNPHNPIVTTNVDRIYNVTVTDPQLGCSVERQIA
                     VRVHPAIGLQTA"
     assembly_gap    868..1012
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1013..>1389)
                     /locus_tag="PK198_01775"
     CDS             complement(<1013..>1389)
                     /locus_tag="PK198_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37489.1"
                     /translation="GLSADFEAILEVGGAQFATGSPATYNITQAGTYQLQVIAADQFM
                     CQDTAIVNVLAADFQPGPLSSPQTICVNTPTPLNPGGNPAYNYTWSPTTGLNLSDPSN
                     PIAVLNAPATYSVTVTDPASGCN"
     assembly_gap    1390..1464
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1465..3560)
                     /locus_tag="PK198_01780"
     CDS             complement(<1465..3560)
                     /locus_tag="PK198_01780"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37490.1"
                     /translation="MSTDYIYRWSPAEGIDDPTSNNPVFSPAQTTTYTLTVGSVGADT
                     CFYDIASLLVIVPPAIDLMADGGGQTCDAEVTLVATAEVPVIFEWTNAGGTVLSNDNE
                     LTVPVSGPATYTVRATDAFGCSQTQDVQVTGGPVNVSVPPVAAVCLGEELELIVANND
                     PNDILTFAWSPTSAFVPGTENNQTPDYIETIGERTVTVDITNQYGCTLTASIPTAVVD
                     TAIQLGFTWQLQCNGATVDFTNTSTNAFAYVWDFGDGTGSTDVNPQHTYTAAGTYTVT
                     LRIAYDVSCVSEFSSQVTVIEPQIVAAFDYDIAACNNGSAEVAFFDVSLNNFNNTTAW
                     NWVFSNGLTSNQQNPVITVNQSGDLIVTLTITTANDCSATITGAVNIRLVEVSLADSI
                     RLCLGDSVQLNPTGNPAYVYQWSPATGLNDPTAANPLAFPTQTTIYTVQIQAFGADTC
                     SFVHQLTVWVPDAIGLDLPDNAVTCGGPVTLGANTIAPATVVWTNSQGTVLGGPTITV
                     DPFRSETYTATATDAFGCTESGSVTVTDHGVDVQVTPSGNITACEDVAFQITSVNLDD
                     QDILIYDWSPDSLIISGDGTPTPTVMIGAAGAMIRGFITNQFGCVDTVEVNVTIVPFT
                     VAIADTVRICAGEPAGLGPDANPAYTYVWSPATDLSNPNIANPIYTGTTGGVYTVTVT
                     DTSTGFLCETVRTVRV"
     assembly_gap    3608..3625
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3626..5883)
                     /locus_tag="PK198_01785"
     CDS             complement(<3626..5883)
                     /locus_tag="PK198_01785"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37491.1"
                     /translation="MKRFHSFLLRAGFTLLLAIAILPGLHATHIVGGQIGYRWISGTT
                     YEVILDVYRDCSNPANEPFDNTAFVGVYNLAGNWITNISMPFLGADTLDAILFDDCLF
                     VPANVCVARTQYKGTITLAANPLGGGYRFSYTRCCRNETLTNIVNPLETGAVYDIVLT
                     TNAMQRQNSSPRFRSWPPIFVCVNNPIFYDHSAVDTFQSANLQDSIVYRLCEPLSGGT
                     LSQPRPIPPQNPPPFDTVDWRMPTYSLFNMLGNNGDTTLVLTGCTSSDTIVSDSLSID
                     PITGLLTGTPTIQGQFVVGVCAEEYDRATGILLSTTRRDFQYNVGPCNEVAANFDAPS
                     VQCDNQTVQFTSTSTNADEFIWYFDFPSPTPSSTLQNPSFTFPDTGTYTVALVVEPLS
                     VCVDTFCTTIRLTNSTLRASFRADVFDCDNFALLQAQDQSADSSVAIVSRLWTVTYNG
                     TTLTSIEANPVFNLPLNVTGTIRLAVTNANNCTEFFESTFTTGLDNPGSFIETSLTAC
                     DGDTLALNPNTDLNISFSYLWSPAAGLLDPATSVNPRIVANGTAVYTVTITAPGNVCQ
                     IVKTVTVNSNPLPDLDFEAMADCDGLTINFNNTSTGATSFEWNFGDPNNPAAGSSLPS
                     PSYTYSDVGTYLVTLVVPASELCRDSIAREVVVLSKSLSAEFDVEYESCSPDSVVVRF
                     VDRSISTVSSISSWSWDFGNGQTSAEQNPTLTFFTDTDLNVTLTITAADNCSNTMSKD
                     VAIRLVDPQDFDG"
     gene            complement(5934..>7084)
                     /locus_tag="PK198_01790"
     CDS             complement(5934..>7084)
                     /locus_tag="PK198_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37492.1"
                     /translation="PAQVNLEVASLRRADREPVNGTTVKLYEVKPDGTEVLVDTQTKK
                     DNHNFTFPLAPGKLYIIRGDHPTYYPTSERVDLRDPATAASGTLKRELLFEQPLPVLT
                     FDAQNRQALPGVRIELRALDGTTSRRVEMKENPKGNDFSFPVLPGRLYTIIAEKPGYQ
                     RAEKTITIPNDPKIKLEPVEIFLKQKNFDEFLPLALYFDNDHPNPRTRQSTTTLEYGQ
                     TFDRYYSRKKEFISEYTKGEQLSESDRFLIEENFENFFERDVNGGYRDLLAFSAKLLD
                     YLREGNSVSIELRGYASPRATNDYNLILSRRRTSSVRNHFYRYENGALLPFINNGSLK
                     IGTVGFGEDTPDAIKASDRYDDLKGSVFSTQASLARRVEILDVKVGGN"
     assembly_gap    7085..7117
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7118..>7433)
                     /locus_tag="PK198_01795"
     CDS             complement(<7118..>7433)
                     /locus_tag="PK198_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37493.1"
                     /translation="TTTERIDLRGNPGRVSARLYLPPTTQQLEVTTFRKPDGTPLSAV
                     LVELFEVKPNGERVFIDSRTNPSNNDVRFPIESGKQYLLKATRPGYLPTTETVDMSSP
                     ALR"
     assembly_gap    7434..7556
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7557..>7919)
                     /locus_tag="PK198_01800"
     CDS             complement(<7557..>7919)
                     /locus_tag="PK198_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37494.1"
                     /translation="DKPCPLTLEVVTLKENLQPLREATVSLFEIAPDGTERLVDSKTN
                     NTSNDYKFLVERGKNYVIRGERELYLPASTTLDLTKPENIPPGNTIRKELIFRQNLNV
                     VDVSTFHAITREDLDSVRL"
     assembly_gap    7920..7932
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7933..>8368)
                     /locus_tag="PK198_01805"
     CDS             complement(<7933..>8368)
                     /locus_tag="PK198_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37495.1"
                     /translation="APENLGLPFNSSYNDVYFVLNEDGTQALFSSNRPGAVFLDKTKE
                     VCCYDIWEIELAKPFKLDVLTFFKPDMSALSGAKVEVYELTPQGEERLVSSLVNPSSN
                     DFSFLLTRGKKYIIKGTKEGLQPDSKIIDLNDPAYANQEGAEV"
     assembly_gap    8369..8384
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8385..9502)
                     /locus_tag="PK198_01810"
     CDS             complement(<8385..9502)
                     /locus_tag="PK198_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37496.1"
                     /translation="MTNRILLLLGCLFGMAAMSYGQSKQAWVTSADSAYARRDFYAAF
                     KYYEAALKYDDSRTDLWYRYAESALEFNAFPSALRAYERVLGSSDKSSYPLTTLRMAG
                     AYQAMGKYDEAQTFYQRFIDEQPGASPADLAKADKGIRDVATARQILSNPGNLSVKNL
                     GDTINSPYSDFGAVYHNDTLYYTSFRFVDRKDMHNPPRPFNKVMTSVKGAKGQPLAEY
                     FNEPGKHVAHSAFNHDYSRVYYTICEYTSVTDVRCDLYYRNRSGQSWGLAQRLSINAA
                     GYTNTEPSVARNAETGEETLFFASNRPGGRGQLDIWMAALQPDGSCSTPVNLTAINTP
                     LDDATPFFHEATQTLYFSSDGHSGLGGYDVFRSMKRGGG"
     gene            complement(9580..>9759)
                     /locus_tag="PK198_01815"
     CDS             complement(9580..>9759)
                     /locus_tag="PK198_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37497.1"
                     /translation="ALIPGIELTYDRWMAGFTYDINISPFNVATNRRGGPEFSLRYII
                     AKVPALGAFKVCPLI"
     assembly_gap    9760..9775
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9776..10671)
                     /locus_tag="PK198_01820"
     CDS             complement(<9776..10671)
                     /locus_tag="PK198_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF37498.1"
                     /translation="MRKQLPAPLKVRLGFLAFIVLMMAGGLSAQDIHYSQYWNAPFNT
                     NPALTGVFRGDTRFTANYRSQWNTVPVEYMTFTGTIDHKFPVRGERNGFFAGGFNFNY
                     DQAGLSRLHLATFGLNGSYTHKLSQGVFATAGVSASFNQRGFKIQDLTFDNQYSNPRG
                     QYDPGLPTGENFPNFNRIYADFGTGLNIRIQNVDAYGSLVDELAKRSRLDIGMGLFHF
                     NRPDQSFIKDQKVPLGMRFSPYAFGVLMLGQDLDLVGNFSAQFQGTYKEMVGMGGLRY
                     HFSTRPGKQAALQLDLGYRFHE"
     gene            11161..>12107
                     /locus_tag="PK198_01825"
     CDS             11161..>12107
                     /locus_tag="PK198_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37499.1"
                     /translation="MKQTNFITLVLILACALTAQAQIFVRSGANRDGSSWQNAFGDLQ
                     QALHAARQRGPVQIWVAEGKYAPTATGNRDASFVIPDGVVLLGGFAGFESSPSQRDWQ
                     KNLTVLSGEIGSPNSLEDNSYTVVLTKNVSAATTIDGFVISSGTANGVVREKGALQRS
                     GAGWYNDGSNGYSNPTVLNCLFINNYGRDGAAFYNYAANGVANPRIENCQFVNNHADL
                     DGGALYNNGSFGISSPTIINCLFEENEASYGGAIFNIAQSGESLPKISRTAFTKNLSY
                     VRGSSVYNNRDTSAGACDADVDSSCSFNSNIDKVGTTVS"
     assembly_gap    12108..12156
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12447..17708)
                     /locus_tag="PK198_01830"
     CDS             complement(12447..17708)
                     /locus_tag="PK198_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF37500.1"
                     /translation="MNRLKFQFAGAPLGVPTPASATTARVLPTVEVAEYEVTMRRSAE
                     EPLIEIDALENDVFEFVMDDDSQWFVRAEDLADNLRRSYQARGVETDPNVIVIPAQFE
                     TQPGTRSLLTEAAKHKLMRFYTNWAGEVAVKIIAEKMESKLRTGLHQLGKNFELLELP
                     DFKTAPSEPYLLLLHGTASDVVGAFKSFTEKDRTTWEKIYGAYEGRVLAFEHKTLSES
                     PLKNTRDLLESLPEGITLDLVSHSRGGLIGEVLARFSEDVELSETLRKAFENKEKALL
                     GELAKVQELIRDKKPKVRKFVRVACPTGGTTLMSKRLDVWLNVMLNSLRLIPGFSGNP
                     VYEFLSDFIKAVVHERFEAKSLPGLAAMMSDSPLVKLLNHSEKKLESSLYVISGDARA
                     GKLFGKLLVLVGDLFFREEHDLVVPVSSMRIGVPRLQPVWEHLSKDEVVNHCNYFLNP
                     GTRQAILTALTAQHLLADDFRELRASVSHLRKIKVAPTHQSGRPTVFLLPGIMGTHLR
                     RDQLRIWVNYAELATGGLMDLKIDDAKIQPDGLHDPTYADLAEYLSRKNYNVIPWGFD
                     WRQDLLQSAAQLKDAISEYLDKEDGQQSKAPVSFIAHSMGGLLLRTTFAKYPELYPRL
                     AARPHFRVLLLGVPLEGSWAIARVLAGRDRLIQKVALLDLIHSKRDLLSVISQYPGFL
                     QLLPRNDLNIQQFFSYWKQNPDEYPLLPDNAQSYLDALRELPEGPSWNARVVKYIAGQ
                     DDQTPDDVLIEGSQLRVRATPEGDGRVPWATIPRELEDATYYVPVSHGEIPDYQPAFE
                     AFAELLDKGSTELLSRRKPVFRSIAAQNWMPEEDVVEYPSETDIDDAVGGRKALLSAA
                     PAAATVQVSIMHGDLAHSRYPLIAGHFKGDAILFAEKVMDGYLDDKLSMRYELGHYAE
                     EIGQNIILIPKEGRFKGGIVIGLGNFGELTASKLQTSLHSALVNYLLKLSEESPGEKG
                     AEVGISTLLIGSGFGSLLIGDCVKAVFDAVLEVNKQARAQSSDKLPLIRHVEIVELYR
                     YKVVQCLRFIRHLSRDARYSAFQLAGQPVRSVAGRRDRIPDEINSEWWHRLKISVADS
                     GAQHDAARFNRPLVFTSITDRSRAEEKILDTNRVIVDELVQEAARFAVANPGLSKALF
                     ELLVPNEFKNYTSELRNMVLIVDKETARYPWELMSSAEKGKDQPIAVKAGLLRQLSTS
                     NFRPQVESAGGNKALVLGDPLLEGMHPQLPGARQEAQNVADILGKNGYDVSAHIGSKS
                     MLAVIDLLSQPYQILHFAAHGISNDPKTGQTGIVLGPNTILTPSVFKQMRYVPDMVFV
                     NCCSLGEIEPEKEKYLQAKYEVAASVGCQLIEMGVRAVIVAGWEVEDLAARHFSELIY
                     QQLLSGVQFGRAVQNARTTVFNEHQGNNTWGAYQCYGDPFYVLKKIRKDNAPSKPILA
                     DAEEAVTELDNLLNGIDTAGMRTADKEALLKKISGWENNVEMFCSDSRVMEKIAEAYA
                     KAGDLKTAVARYEQVFRSETAKYSVRAIEQCFNLRVRLLAEQAELDADGLNIEDTIGQ
                     LNGYIQEMEAFNRIYPSSERLSLVGSAYKRAFMIHPQTESLKKAEKAYYESWQYDRRH
                     PDRRSNMYYPFFNWAMAYALLEVAGQNLPQHKPAEADLKQALAEAEALDAQKPDFWNK
                     TAPSMGYAYQLIKAKDAAKRKRLIEQLEDNFREAWKTEGNESNRKSHLDQFRFIICAL
                     SLKKDEKAVAAKIAAFEEVMAGIGRIR"
     gene            17971..20112
                     /locus_tag="PK198_01835"
                     /pseudo
     CDS             17971..20112
                     /locus_tag="PK198_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            20239..20484
                     /locus_tag="PK198_01840"
     CDS             20239..20484
                     /locus_tag="PK198_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HRF37501.1"
                     /translation="MQLYLRDELASVARPVMELKGFQRLHLLPGESREVRFPIGPEQL
                     RMLDEQLRPVIEPGAFSILIGASSQDIRQKAVLMVGR"
     gene            complement(20542..>20826)
                     /locus_tag="PK198_01845"
     CDS             complement(20542..>20826)
                     /locus_tag="PK198_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRF37502.1"
                     /translation="TATIIAAEPVKGADKLLQLTVDLGFEQRTVVSGIALHFRPEAVV
                     GQRVVLVANLAPRKLRGVMSQGMILMAEDAAGKLSFVSAPEGAGNGMIVK"
     assembly_gap    20827..20871
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20872..22695)
                     /gene="metG"
                     /locus_tag="PK198_01850"
     CDS             complement(<20872..22695)
                     /gene="metG"
                     /locus_tag="PK198_01850"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRF37503.1"
                     /translation="MPDNRYLITSALPYANGALHLGHLAGAYLPADIYVRYLRLMGKD
                     VVWVCGSDEHGAAITIRAKKEGISPQEIIDKYHALNKDTFARLGISFDYYHRTSEALH
                     HATAQDFFTRLHEKGDEFEVRSSEQYYDEDFKQFLADRYIKGTCPKCGHTEAYGDQCE
                     KCGSDLSPTELIDPISTLSNKTPVLRATQHWYFKLDKHGEWLRRWIETGELDGQQHHD
                     AREWKKPVIGQCMSWLNEGLQPRAITRDLDWGIPVPLPEAAGKVLYVWFDAPIGYISS
                     TKQWALENGKNWEDYWRNPDTKLIHFIGKDNIVFHCIIFPAMLYAYGGYNLPVNVPAN
                     QFLNFEGEKFSKSRGWGLEQHEYLEEFKDFPNAEDALRWSLTRNMPENGDSDFKWDLF
                     VDFHDKELADNLGNFVNRVIVLSHKYFNGAVPQPTGADADKLAAAQQLAKNIAAELEA
                     FSFKNAAQMLMELSSFGNTYLQDVSPWALWKQDPQHPAIAECLYVCIQISELLALLSE
                     PFLPFTSPRIRRLLGHAPVSNGDLNAAFEAMAQGQALATPGHVLGQPEVLFPKIMDRN
                     DKSRWEIVERQKNKLSALMLPEQTTTPPQPEAQSQEPEAVGL"
     gene            22898..25324
                     /locus_tag="PK198_01855"
     CDS             22898..25324
                     /locus_tag="PK198_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37504.1"
                     /translation="MKKKTFTLLIAICLAHFASAQNAPTHEQAYGYIGSNGNMRVIKP
                     AFLAGQYFAGGLMNNEATIIRVNRAGQALSHRQLNLPGATQSEITDAALVSATLLAVA
                     GTCTGCMSAPDRKGVFLMLFDQHLNEAATLLLEPNEQQAHFENARLAVGNNTLYLAFN
                     DGFFGGSHHIRAYDLNLNQLWASFQHLAFIESPVKLFFQGNELRLIANEWTGNTNTGT
                     RLIRLNASTGVPAGHYAYAAYAIDAAELPDGNYAIAAAGTFYPGDQKNKIFVVSPLSG
                     EVLDSTLVGHHGNSRITAFDALPNGQLMAALNEFAFFEDTLKFIRFDPADLEAPVSER
                     IIWGGRGAKERQVYDFLPLNQDGSSYLSVGWRSDVSTRGMLLAALPGGFTLQAPAQWN
                     NDICGSDLLQSPYQQHYPSVLPIIHENVVYQANAILYNGQVRDLSMDIYVPLDLYSTG
                     QSTQKRPLLMFVHGGGFIGGHKDVFSSYALAFAETGYVTASINYRLGVADGVNSLSQF
                     CDHTEAVFAAMYRATQDTRRAIQFLYDHADDYHIDRNKIFVLGHSAGAINVLNSGLLD
                     ADELPYPFESQLGPLPPKPPVAAYLPWAGAIASLNMIDTDEDTPVMFIHGTCDPLVPY
                     GEGNLICPDLPFGFGSRAIAARKKELCHDYLLLGIEYGDHGGGGSDLDILYRIIDWLK
                     NQNICGTPTQECEVISAANPGNCPPQTMCPDTEPCSEPTSALNVPEAPVVEIFPNPAT
                     ERVTISLSNGAVFKGSLAIYNTDGQRIRSYPPNENTRIDLDISDLPAGVYLVEMFVAG
                     NTMRHKLIIL"
     gene            25339..>27412
                     /locus_tag="PK198_01860"
     CDS             25339..>27412
                     /locus_tag="PK198_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRF37505.1"
                     /translation="MKTLYTLFFGLYSFCAAAQYCGPLHVHPGATGAGTGESWQDAFV
                     HLQDALDAAQPGAQIWVAAGVYLPQKDTTGNATPANDRSKTFFINKDIQLYGGFAGAE
                     QELSQRDPAANPTILSGDLGTTGIATDNAYHVLWLALVSPLCIIDGFTITRGNANASD
                     VNGTGGGILLRHQPLQYAAPVIQNCIIEHNHATTGGGFYTATDSWAGPSVSIEHCKFR
                     NNTASSGGGIYFQQSMVAEVGLQLTNVEFSDQSIGQYGGGLYANGHFTARDCRFENNS
                     SNRGAGANLEGGTAILENCAFIGNTSLRNGGGLRMERTTATVVNCLFLGNEANILNES
                     STTSNAGGGMFYRGSNTSFAKLANCVFSGNKGQNGAGFAQTATVLNTSSAELDNCTFA
                     GNNGASAVARLQGNLTIENSIIFGNEGTALTGGTARSCIIEGGYAGTDNVSVDPLFVA
                     LPSFLLAPTAAGNLKLQSGSPAIDQGDNALTPTGFITDADGNHRMINCTIDRGAFEFG
                     TSGASDRICYADADGDGLGNAAMPRLACNTCPGGFVSNNGDCDDSNSAITHTLPPPAA
                     IQLTTEFCRGEPSITLRSIQSATPGRLVWVLVQAPPGSRFEGQEPLEFHPGAVNAEFS
                     TGTDNLLMCSTPSYQNTSVNGVWMFETYYETAAGCRSAVQQGFSITVRQTSTTQLSTT
                     ACNSYTFNG"
BASE COUNT         6047 a         7271 c         7335 g         6226 t
ORIGIN      
        1 cggctgtttg taaccctatg gccggatgta cccgaacggc tatctgcctc tctacactgc
       61 atcccaactg cgggtcggtg accgtgacgt tgtagatgcg gtccacgttg gtcgtcacaa
      121 tcgggttgtg cgggttgctc agattcaggc cggtagtcgg cgaccaagtg taggtataag
      181 acggattacc ctccggattg atcggcgtgg gcacgtctat acacacccgg atagaatccg
      241 gcaatcctgt attgatctcc accactgtaa cattgaccga tgtagtatcg gcacacccat
      301 tggtatcctg tgccaccaca atgtagttgt tgctgccttt ggccggcgtc accgtgatgc
      361 tcgcacccga acccacaggg ttgttgaagg cattgaggaa ccaactgtaa ctcacattca
      421 cggaagccgt agccgtcagc gtcacgggcg caatttcgca caataccgtg tcggggcggg
      481 cttccagatt gatgagcggg aagacattca ccaccatctg ctgctgaacg ctacagccgc
      541 tcgccggatc ggtcaccgtc acattgtagg tagtcgaggc attcaacacc gcaatcgggt
      601 tggagggatt gctcagatca aggccggtag tgggcgacca tgtgtaattg taagccggat
      661 tgccgcccgg attgagcagc gtggccgtgt tggcgcagat cgtttgcgga gaagtcagcg
      721 gaccgggttg gaaattggct aaattaatcg tcaccgaagc ggtatcccga caaccattgg
      781 cgtcttctgc aatagccgtg taagaatatg tacccggcgt gagaaaactg agtggaaacg
      841 aactgcccgt gaaaataggc gttgtgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncattgcag
     1021 ccgctggccg ggtcggtcac cgttacgctg tacgttgcag gagcattgag tacggcaatc
     1081 ggattggacg gatcgctcag gttgaggccg gtcgtcggcg accaagtgta attgtaagca
     1141 ggattgccgc ccggattgag cggtgtgggc gtattgacac agatcgtttg tggcgaactc
     1201 agcgggccgg gttggaaatc ggcagccaat acattcacaa tggccgtatc ctgacacata
     1261 aactgatcgg cggcaatcac ttgcagttgg taagtgcccg cttgtgtaat gttgtacgtc
     1321 gcggggctgc ccgtagcaaa ctgtgcgccg cccacttcta gaatggcttc gaaatcggct
     1381 gagagaccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnatgact cgcaccgtcc gcactgtttc acacaaaaat
     1501 cctgtagaag tatcggttac cgtcacggta taaacgccgc cggtagtgcc ggtgtaaatc
     1561 gggttggcta tattgggatt gcttaaatcc gttgccggcg accatacata agtataggcc
     1621 gggttggcgt caggccccaa gccggcaggt tcgcctgcac agatgcgcac tgtatcggca
     1681 atagctacgg tgaacggtac aatggtcaca ttcacttcca ccgtatctac acagccaaac
     1741 tgattggtaa taaaccctct gatcatcgct ccggcggccc cgatcatcac ggtgggcgtg
     1801 ggcgtgccgt cgccgctgat aatgagcgaa tcaggactcc agtcgtaaat caaaatatcc
     1861 tgatcgtcga gattgacgga agtaatctgg aaggccacat cctcgcaggc agtgatattg
     1921 ccggaaggcg tcacttgcac atccacgccg tgatcggtga ccgtcacgct gcccgattcg
     1981 gtacaaccga aagcatcagt agccgtagca gtgtaggttt ccgacctgaa cgggtccacc
     2041 gtaattgttg ggccgcccag cactgtaccc tggctattgg tccaaactac cgtcgccgga
     2101 gctatcgtat tggcgcccaa agtcaccggg ccgccacaag tcacggcatt gtcgggcagg
     2161 tccaagccga tggcatcggg cacccacacc gtcagttgat gcacaaaaga gcaggtatcg
     2221 gctccaaaag cctgaatctg aacggtataa atcgtcgttt gagtcgggaa cgccagagga
     2281 ttggcagccg tgggatcgtt caggccggta gccggcgacc actggtaaac ataagccgga
     2341 ttgcccgttg gattcagttg caccgaatcg cccaaacaga gccggataga atctgccagg
     2401 ctgacctcca ccaaacggat attgacggcg cccgtaatgg tagccgagca gtcgttcgca
     2461 gtggtaatgg tgagcgttac gatcagatcg ccgctttgat tcaccgtgat gaccggattt
     2521 tgctggttcg aggtcagccc attgctgaac acccagttcc aggcggtggt attgttgaag
     2581 ttgttcagtg aaacgtcaaa gaaggctacc tctgcgctgc cgttgttgca ggcagcaatg
     2641 tcataatcga aagctgctac aatttgcggc tcaatgaccg tcacctgaga ggaaaactcg
     2701 ctcacacaac tcacgtcgta agcaatgcgg agtgtaacgg tataagtacc cgcggcggtg
     2761 taggtatgct gcgggttcac atccgtactt cctgttccat caccgaaatc ccacacataa
     2821 gcgaacgcat tggtgctggt gttggtaaag tccaccgtag cgccgttgca ctgcaattgc
     2881 caagtgaaac cgagctgaat ggccgtatcc actactgcgg tcgggatgga agccgtgagc
     2941 gtgcagccgt attggttggt aatatctacc gtcacggtgc gctcgcctat ggtttctatg
     3001 taatccggtg tctggttatt ttcggtgccg ggcacaaaag cacttgtcgg cgaccaggca
     3061 aaggtgagta tgtcgttggg gtcgttgttg gcaacgatta gttccaattc ctcgcccaaa
     3121 cacacagcgg ccacaggtgg aacgctcaca tttacagggc ctccggttac ctgcacatct
     3181 tgtgtctggc tacagccaaa cgcatccgta gcgcgcaccg tataagtggc cggcccggaa
     3241 acaggcaccg tcagttcgtt atcgttcgac aaaactgtac ctccggcatt tgtccattca
     3301 aatatcaccg gcacttcggc agtagccacc aatgtaacct cggcatcgca ggtctgtccg
     3361 ccgccgtcgg ccatcaaatc tatggcaggc ggcacaatga ccaacaaaga ggcgatgtcg
     3421 taaaaacaag tatcagcgcc cacactaccc accgtaagcg tgtaggtagt tgtttgcgca
     3481 gggctgaaca cagggttatt ggacgtagga tcgtcaatgc cttccgccgg agaccagcga
     3541 taaatatagt cggtgctcaa aggcgccggt agttgtgtgg tactgcccaa acaacgcacc
     3601 aactgagnnn nnnnnnnnnn nnnnnccgtc gaagtcctgt gggtccacca atctaatagc
     3661 cacatctttg ctcatggtgt tgctgcaatt atctgcggct gtgatggtca gcgtgacgtt
     3721 cagatcggta tccgtaaaga aagtcagtgt cgggttttgc tcggcagagg tctggccgtt
     3781 gccgaaatcc caagaccagg aactgatgct gctcactgtg ctgatggagc gatccacaaa
     3841 acgcaccacc acgctgtcgg gcgagcagga ttcgtattct acatcaaatt cagcgctgag
     3901 actcttgctg agaacgacca cctcgcgagc aatggaatcg cggcacaatt cagatgcagg
     3961 tactaccaaa gtgaccaaat atgtgcccac atcggagtaa gtgtaagatg gactgggcag
     4021 gctggacccc gcagcaggat tgttcgggtc gccgaaattc cattcaaaag atgtagcccc
     4081 ggtgcttgtg ttgttaaaat tgatggtcag cccgtcgcag tctgccatcg cctcgaagtc
     4141 cagatcaggc agcggattgc tgttcacggt tactgttttt actatctggc acacattgcc
     4201 cggcgcggtg atcgtcactg tataaacagc cgttccgttg gcaactatac gaggattgac
     4261 ggatgtagcc gggtccaaca ggccggcagc cggcgaccac aggtacgaaa agctgatatt
     4321 gagatcggta ttggggttca acgccagggt atctccatcg caggccgtca gagaggtttc
     4381 aatgaaacta cccggattgt ccaaacccgt agtgaaagtg gattcaaaaa attcggtaca
     4441 attattggca ttggtcactg ccagccgaat tgttcccgtc acattgagcg gcaggttgaa
     4501 tacggggttc gcctctatgg aagtaagcgt agtaccgtta taagtcaccg tccaaagccg
     4561 cgacacaatc gcaaccgagc tgtccgccga ttgatcctgc gcctgcaaaa gggcgaagtt
     4621 gtcgcaatca aacacatcag cgcggaaaga tgcccgtaaa gtgctgttgg tcaggcgtat
     4681 cgtggtacaa aatgtatcaa cgcacacgct caaaggctcc accaccaaag cgaccgtgta
     4741 ggtgcctgta tccgggaagg taaacgatgg attctgaagc gtagaacttg gcgtgggcga
     4801 ggggaagtca aaataccaga taaactcatc tgcgttggta ctggtactgg taaattgcac
     4861 agtctgattg tcgcattgta ccgagggcgc gtcaaaattg gcagccactt cattacacgg
     4921 ccccacgttg tattggaagt ctcgtcgggt agtagaaagc aatatccccg tagcccgatc
     4981 gtattcttct gcacaaaccc ccaccacaaa ttgcccctgt atagtaggcg tacctgtgag
     5041 caagcctgtt atggggtcaa tgctcagaga atcagataca atggtatcgg atgaggtaca
     5101 tccggtaagt accagcgtag tatcgccgtt gttgcccagc atattgaaca ggctgtaagt
     5161 aggcattcgc cagtccaccg tatcgaaagg cggtgggttt tgaggcggaa tgggccgggg
     5221 ctgactcaaa gtgccgcctg aaagcggctc gcacaaacga tacacaatgg agtcctgtaa
     5281 attagctgac tgaaaagtat ccactgccga atggtcgtaa aatatcgggt tattgacaca
     5341 cacaaatatc ggcggccagg agcggaaccg ggggctgctg ttttggcgct gcatggcatt
     5401 ggtggtaagt acaatatcat aaacggcgcc tgtttccaac ggatttacga tgttcgtaag
     5461 tgtttcgttg cgacaacaac gcgtataaga aaagcgatac cctcctccca atggattggc
     5521 agccagcgta atcgtgcctt tgtactgggt gcgggccaca caaacattgg ccggcacaaa
     5581 aaggcagtcg tcaaacagaa tggcatctaa cgtatccgca cccagaaagg gcatggaaat
     5641 attggtgatc caattgccgg ccagattata gacccccaca aacgctgtgt tgtcgaaagg
     5701 ctcgttggcg gggttgctgc aatcccgata tacatcgaga atgacctcat aagtagtacc
     5761 cgaaatccag cgatagccaa tctggccgcc tacgatgtgc gtggcgtgca gccccggcag
     5821 aatggctata gccaacagca gggtgaaccc cgcacgcaac agaaaggaat ggaagcgttt
     5881 cacggcattt gtgttttgaa cgatcatgga attaccgatc attgtattaa tttttagttt
     5941 cctcctactt taacatccaa aatttccacc cgacgggcca gagatgcctg cgtactgaac
     6001 accgagcctt tcaggtcgtc gtagcggtcg ctggctttga tggcatcggg cgtatcctcg
     6061 ccaaacccga cagtgccgat tttcaggctg ccgttgttga taaacggcag cagcgcgccg
     6121 ttctcgtagc ggtaaaaatg attgcgcacc gaactggtcc tgcgacggct cagtatcagg
     6181 ttgtaatcat tggtagctct tgggctggcg tacccgcgca attctatgga aacggaattg
     6241 ccttcgcgga ggtagtccag taatttggcc gaaaacgcca gcaagtcccg atagccgcca
     6301 ttgacatccc gctcgaagaa gttttcaaag ttttcttcta tgaggaagcg gtcgctctcg
     6361 ctgagttgct cgcctttcgt atattcactg atgaactcct tcttgcgact gtaatagcgg
     6421 tcaaaagtct gtccgtattc cagcgtggta gtgctttgtc tggtacgcgg gttgggatgg
     6481 tcgttgtcga aatacaatgc caaaggcaaa aactcatcga agtttttctg cttcaggaat
     6541 atttcaacag gttccaactt gattttcgga tcattaggaa tggtaatcgt tttttcagcg
     6601 cgctgatagc ctggtttttc cgcaatgatg gtataaagac gaccgggaag taccgggaaa
     6661 ctgaagtcgt tccccttggg attttccttc atctccaccc tgcgcgaggt agtgccgtcc
     6721 aaagcgcgca actctatgcg tactcccgga agcgcctgac ggttttgagc gtcgaaagtc
     6781 agtaccggca agggctgctc aaacagcaac tcccgcttca gcgtacccga agccgctgta
     6841 gccgggtcgc gaaggtccac gcgttccgag gtgggatagt acgtcggatg atcgccgcga
     6901 atgatgtaca acttgccggg cgcgagcggg aaagtgaaat tgtggttgtc ttttttggtt
     6961 tgtgtgtcca ccaatacctc agtaccatcg ggttttactt cgtacaattt cacggtagtt
     7021 ccgttcaccg gctcccggtc tgcacgacgc aaggacgcca cttccagatt gacctgcgcc
     7081 ggctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg caaggccggg ctgctcatgt
     7141 ccacggtttc ggtcgtgggc aggtagccgg ggcgcgtagc cttcagcaaa tactgcttgc
     7201 cggattctat tgggaagcgc acatcgttgt tgctcggatt ggtgcggctg tcaatgaaca
     7261 cgcgctctcc attaggcttg acctcaaaca gttctaccaa tactgcggaa agcggcgtgc
     7321 cgtcgggctt gcggaaagtg gtcacttcca actgctgagt ggtaggcgga agatacagcc
     7381 gggcgctcac ccgaccggga ttgcctctca gatcaatgcg ctccgtcgtc gtannnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggc
     7561 gtacgctgtc taagtcttct ctggtaatgg catggaaagt actgacatcc accacgttga
     7621 ggttctggcg gaagatcagt tctttgcgaa tggtgttgcc cggcggaata ttttcgggtt
     7681 tggtcaaatc cagcgttgtg gaggccggga ggtacagttc ccgttcgccg cgaatgacgt
     7741 aatttttacc gcgttctacc agaaatttgt agtcattgga ggtattgttc gtcttgctgt
     7801 ccaccaagcg ttctgtgccg tcgggggcaa tttcaaacaa agaaaccgtg gcctcgcgca
     7861 ggggctgcag gttttctttc agcgtcacta cttccaatgt gagcggacat ggcttatcgn
     7921 nnnnnnnnnn nnacctctgc gccctcctga ttggcataag ccggatcgtt gaggtctata
     7981 attttggaat cgggctgcaa accttccttc gtgcctttga tgatgtactt tttgccgcga
     8041 gtgagcagga atgaaaagtc attggaggaa gggtttacca acgaactcac caagcgctct
     8101 tcgccctgcg gcgtgagttc atacacttct actttagcgc ctgaaagcgc cgacatgtcg
     8161 ggtttgaaaa aagtcaaaac atccaatttg aagggcttgg ccaattcgat ttcccaaata
     8221 tcatagcagc acacttcttt ggttttatca agaaaaacag cgccgggccg gttggaagaa
     8281 aacagcgctt gggtgccgtc ttcgttcaac acaaaataca catcattgta gctgctgttg
     8341 aacggtagtc ctaagttttc aggcgctgnn nnnnnnnnnn nnnncctcct ccgcgcttca
     8401 tggagcggaa tacgtcgtat cctcccagcc cggaatgccc gtcggaactg aaatacagcg
     8461 tctgagtagc ttcgtggaag aaaggcgtag catcgtccaa cggcgtgttg atggcggtca
     8521 ggttcaccgg cgtagagcaa gagccgtcgg gttgcagggc tgccatccag atgtccaatt
     8581 ggccgcggcc gccggggcgg ttggaagcaa aaaagagggt ttcctcaccg gtttcggcat
     8641 tgcgggccac cgagggttct gtgttggtat agccggccgc attaatactc aagcgttgcg
     8701 ccagccccca gctttgcccg ctgcggttgc ggtagtacaa gtcacaacga acatccgtca
     8761 cggaggtgta ttcacagatt gtgtaatata ctctggaata atcgtgattg aaggcagagt
     8821 gcgccacatg tttgccgggt tcgttgaaat actcggccaa gggctgccct ttagcgcctt
     8881 ttacagaagt catcaccttg ttgaaaggtc ggggcgggtt gtgcatgtct ttgcgatcta
     8941 caaaccgaaa actggtgtag tataaggtat cgttgtgata cacggcgccg aagtcggaat
     9001 acggcgaatt gatggtatcg cccaggtttt tcaccgacag attgccggga ttgctcagta
     9061 tctgccgggc cgtcgccaca tcacggatgc ctttgtcggc tttcgccaaa tcagcaggcg
     9121 atgcgccggg ttgctcatca ataaaacgtt gataaaaagt ttgggcctcg tcgtatttgc
     9181 ccatcgcctg atacgcgccg gccatgcgca acgtcgtcag cgggtaactg cttttatccg
     9241 aactgccgag tacgcgctcg taagcgcgca atgcagaggg aaaagcattg aattccaacg
     9301 ccgactctgc atagcgatac cagagatcgg ttctggagtc gtcgtatttc agcgcagctt
     9361 catagtattt aaaggcagcg taaaaatcgc gccgggcgta ggcgctgtct gcggaggtta
     9421 cccaggcttg tttggactgc ccgtagctca tggcagccat tccgaacaaa catcccaaga
     9481 gtaaaagtat tctgtttgtc ataaagtcgg ttctttgatt cggtttttag aaatgcagga
     9541 ggcaagattc cggctcgggg ccgaaagcag atacatcact caaatgagcg gacacacctt
     9601 gaatgcaccc agtgcaggta ctttggcgat gatgtaacga agcgagaact ccgggcctcc
     9661 gcggcgattg gtcgccacat taaagggaga gatattaatg tcgtaagtga atccggccat
     9721 ccagcgatcg taggtcagtt cgataccggg aatcagtgcn nnnnnnnnnn nnnnnatttc
     9781 gtgaaaacga tagcccaggt ccaattgtaa agcagcctgt ttgccggggc gggtgctgaa
     9841 atggtaccgc aatccgccca tacccaccat ttctttgtag gtgccctgaa actgagcgct
     9901 gaaattgccc accaaatcaa ggtcttggcc cagcatgagt acaccgaaag cataggggct
     9961 aaatcgcatg cccagcggaa ctttctgatc cttaataaag ctctgatccg ggcggttgaa
    10021 gtgaaacaag cccataccga tgtccaaacg ggaacgcttg gccaattcat ccaccaacga
    10081 gccgtaagca tctacattct ggatgcggat gttcaggccg gtgccgaaat cggcataaat
    10141 tcgattgaaa ttagggaagt tttcgccggt aggaaggccg gggtcgtatt gcccgcgagg
    10201 gttgctgtac tggttgtcga aagtcaggtc ttgtatttta aacccgcgct gattgaacga
    10261 tgccgagacg cccgccgtgg caaatacgcc ctgagacagc ttgtgtgtgt aggaaccgtt
    10321 cagtccgaaa gtagccaaat gcagccgcga caaacctgcc tgatcgtagt tgaaattgaa
    10381 ccctccggca aaaaagccgt tgcgctctcc ccgcaccgga aacttgtggt ctatggtgcc
    10441 ggtaaaggtc atatactcta ccggtacggt attccactgg cttcggtaat tggccgtaaa
    10501 acgggtatcg cccctgaaca cgcccgtcaa tgccggattg gtattgaacg gagcattcca
    10561 atattgagaa tagtgaatgt cttgtgcaga aagccctccg gccatcatca gaacgatgaa
    10621 cgccaagaaa ccaagcctca cttttaaagg agcaggtaac tgttttctca tatttttttg
    10681 cttttacaat tatgaacgat tgtgaaaact cagcaaatgt agtgtttttg cttccgcaaa
    10741 tcccatagca acgcccaaat aattatctgc gccgtacaat cacgacgtcc tgtgaaagat
    10801 cgttgatgtt taccatgaga caagaccgga agatagcccg aaaattatcc gactaagcga
    10861 aaccaaacgc gattgtgcct ggcctgcgga aagcaaagct caaaaataca ggctttctga
    10921 cgaaaacacc agtctgagcg tcactttttt ttcttttttt aaaaaaactt ctccaaacgc
    10981 ttgttttcaa ataataaaaa ggcatatatt tgcgccattc ctatattttt gaaaaagagc
    11041 acattgctct gtccaagttt gcagcatctc gtttggcttt tttaaagcca ttgtaggtat
    11101 tcatttccct gcttatctca caacggcatt gatttgaacc gacttcaaaa acgagataac
    11161 gtgaaacaga caaattttat cacacttgta ctcatcttgg cttgcgcact gactgcccaa
    11221 gctcaaatct tcgtacgctc cggcgccaac agagacgggt catcttggca aaacgctttc
    11281 ggcgatttgc aacaggcgct tcatgcagcc cgccagcgcg gccctgtcca gatttgggta
    11341 gccgagggca agtacgctcc tacagccacg ggcaaccgcg acgcttcttt tgtcattcct
    11401 gacggcgtgg tgctgttggg cggctttgcc ggtttcgaaa gcagcccttc gcagcgcgac
    11461 tggcagaaga acctcaccgt gctgagcggc gaaatcggtt ctcccaactc tctcgaagac
    11521 aattcctata ccgtagttct gaccaaaaac gtgagcgccg ctaccacgat tgacggcttc
    11581 gttatcagct ccggcaccgc caacggcgtg gtgcgtgaga aaggcgctct gcaacgcagc
    11641 ggcgcaggct ggtacaacga cggctccaac ggatactcca atcccaccgt cctgaactgc
    11701 cttttcatca ataactatgg ccgcgacggt gctgctttct acaactacgc agccaacggt
    11761 gttgccaatc ctcgcatcga aaattgccaa tttgtgaaca accacgccga tctcgacggc
    11821 ggcgcgctgt acaacaacgg cagcttcggc atcagctctc ctaccatcat caactgcctt
    11881 tttgaagaaa atgaggcttc ctacggcggc gccatcttca acatcgctca aagcggcgaa
    11941 tcgctgccga aaatctcccg cactgcgttc accaagaacc tctcttacgt ccgcggcagc
    12001 agcgtttaca ataaccgcga cacttccgcc ggcgcctgcg atgccgatgt ggacagcagt
    12061 tgctctttca acagcaatat cgacaaagta ggcactacgg tcagcagnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaacg tttccgaaat cgtttttaag
    12181 accaatatgt aatttttttt cgcgccgaac atcaaacaaa acggggggaa tgatcgaaag
    12241 gtcgttccct cgttttgttt ttggggtatg ccctgccaac caagtgaggc gctaaccgaa
    12301 agtcagcgcc tcacgccgcc aggtcaggcg gtaacttccg gttacctccc gacttcaccc
    12361 tgccctaccc gttgcacaag cactctacga ttacgaatcc tgaaaggctt cgcaatcgcc
    12421 tcactgcccg cgcttatccc gccccgtcaa cggatgcgcc caatgccagc catgacctcc
    12481 tcaaaagcgg cgatttttgc tgcaacggcc ttttcatctt tcttcaggct cagggcgcag
    12541 atgatgaagc gaaactgatc caaatgcgat ttgcgattcg actcgtttcc ttctgttttc
    12601 caggcttcgc ggaagttgtc ttccaattgc tcgatcaggc gctttctctt ggccgcatcc
    12661 ttggctttta tcaactgata ggcatagccc atggaaggcg cggttttgtt ccaaaaatcg
    12721 ggcttttggg catctaaagc ctcggcttcg gccaatgcct gtttcagatc ggcttcggca
    12781 ggcttgtgtt gcggcagatt ttgacccgct acttcgagca gggcgtaggc catggcccag
    12841 ttgaaaaaag gataatacat attggaccgc ctgtccggat ggcgccgatc atactgccag
    12901 gattcgtaat atgctttttc cgcttttttg agagactccg tttggggatg gatcatgaat
    12961 gcccgtttgt atgcagaccc aacgaggctg agccgttcgg aacttggata aattcgattg
    13021 aaagcttcca tttcctgaat gtagccgttt aattggccaa tggtgtcctc tatgttcaaa
    13081 ccatcggcat ccaactcggc ctgttcggcc agcaggcgca cgcgcagatt gaagcattgc
    13141 tcgatggcgc gcacgctgta cttagctgtt tctgacctaa atacttgctc ataccgggcc
    13201 acggcggttt tcaaatcacc ggccttggca tacgcctcgg cgatcttctc catcacccga
    13261 ctgtcggagc aaaacatttc aacgttgttc tcccaaccac tgattttttt gagcagggct
    13321 tctttgtcag cagttctcat gcccgcagtg tcaatgccgt tgagcaaatt gtccaattcg
    13381 gtcacggctt cctctgcatc ggccaggatc ggcttggatg gcgcattgtc tttgcgtatc
    13441 ttcttcaata cataaaaagg atcgccgtag cattggtagg cgccccaggt gttgttgccc
    13501 tgatgttcgt taaaaacagt tgttctcgca ttctgaacgg ctcgtccaaa ttggacaccg
    13561 ctcagcaatt gctgataaat aagctctgaa aaatgccggg cggccaggtc ctctacttcc
    13621 caaccggcca caatgacggc ccgcacgccc atttctatga gttggcagcc cacgctcgcc
    13681 gccacttcgt atttggcctg taggtacttt tccttttcgg gttcgatttc ccccagcgag
    13741 cagcaattga caaacaccat gtcaggcaca tagcgcattt gtttaaaaac agagggcgtg
    13801 aggatggtat tcggccccag aacgatgccg gtctggccgg ttttggggtc gttgctgatg
    13861 ccatgcgcgg caaaatgcag gatttggtaa ggctgcgaaa ggaggtcaat cacggcgagc
    13921 atagacttgc tgccgatgtg tgccgatacg tcgtagccgt ttttgcccaa gatgtcggcc
    13981 acattttggg cctcttgccg ggcgccgggc aattgcgggt gcatgccctc cagcagcgga
    14041 tcgcctaaca cgagtgcctt gttgccacct gcgctttcta cctgaggacg gaaattggag
    14101 gtactcaact ggcgcagcag gcccgcctta acggcaatgg gctggtcctt tcccttttcg
    14161 gcgctgctca tgagttccca ggggtagcgg gcagtttctt tatccacaat cagcaccatg
    14221 ttgcgcaatt cggaggtata gtttttgaat tcattgggca ccaacaattc aaaaagcgct
    14281 tttgacaggc cgggattggc aactgcaaaa cgggctgcct cctgcaccaa ttcatctaca
    14341 atgacgcgat tggtatccaa tatcttttct tccgccctcg aacggtcggt gatggaagta
    14401 aacacgagtg gcctgttgaa gcgggcagcg tcgtgctgag cgccgctgtc tgccacactg
    14461 attttgaggc gatgccacca ttccgagttg atttcgtcgg gaatgcggtc tctccggccg
    14521 gcaacagacc tgaccggctg tccggccaat tgaaaagcgc tataccgggc atcgcgcgaa
    14581 aggtggcgga tgaagcgcag gcactgcacc actttgtagc ggtacaattc cacgatttcc
    14641 acatgccgga tgaggggcaa tttgtctgaa ctttgcgccc tcgcctgctt attgacctcc
    14701 aataccgcat cgaaaaccgc cttgacgcaa tctccgatca gcaggctgcc gaagccagaa
    14761 ccgatcagca gggtggaaat tccgacctcg gctccctttt caccgggcga ttcttccgac
    14821 agtttgagca gataattgac caaagcagaa tggaggctgg tttgaagttt ggaggccgtc
    14881 agttcgccga aattacccaa accgatgaca atgcctccct tgaagcgccc ttctttggga
    14941 atgagaatga tgttttgccc tatctcctcc gcatagtgcc ccagctcgta acgcatgctc
    15001 agcttgtcgt ctaagtaccc atccatcact ttttctgcaa aaagtatggc gtcgcccttg
    15061 aaatggccgg cgatgagcgg gtacctggag tgagccagat cgccatgcat aatgctgact
    15121 tgcacggtgg ccgccgcagg tgcagccgac aaaagggcct ttctgccgcc aacggcatcg
    15181 tctatatccg tttcggaagg gtactccacc acatcctctt cgggcatcca attttgagcg
    15241 gcgatggagc ggaaaaccgg tttccggcgc gagagcagct ccgtgctgcc cttgtccaaa
    15301 agttcggcga aagcctcaaa tgcgggttga tagtctggaa tctcgccgtg cgaaaccggc
    15361 acataataag tggcatcttc cagttctctg ggaatggtgg cccagggtac gcggccgtca
    15421 ccttcgggcg tggcgcgaac ccgcaattga ctgccttcta tgagcacatc gtcgggcgtt
    15481 tggtcgtcct gaccggcaat gtatttgacc accctcgcat tccaggaggg gccttccggc
    15541 aattcccgga gcgcatccag gtagctttgc gcattgtcag gcaagagtgg atactcgtcg
    15601 ggattctgtt tccaataact aaaaaactgc tggatgttga ggtcgttgcg gggcagcaat
    15661 tgcaggaagc ccggatattg ggaaatgaca gacaacaagt cccttttgct atgtatcaaa
    15721 tcgagcaggg ccactttttg aatgaggcga tctcgtccgg ccagcacgcg ggcaatggcc
    15781 caggagcctt ccaaaggcac gcccagcaac aagacccgga aatgcggccg tgcggccaaa
    15841 cgagggtaca attcggggta tttggcaaaa gtcgtgcgca gcaacaggcc gcccatagag
    15901 tgggcaatga aagagacggg cgctttgctc tgttggccgt cttctttgtc caaatattcc
    15961 gaaatcgcat ccttcaattg cgctgcactt tgaagaaggt cttgcctcca atcgaaaccc
    16021 cagggaatca cgttgtaatt tttgcggctc agatactcgg ccaggtcggc gtaggtggga
    16081 tcgtggaggc cgtcgggttg gattttggcg tcatcaattt tcaaatccat caagccgccg
    16141 gtggccagtt ccgcatagtt cacccatata cggagttggt cgcggcgcag gtgtgtgccc
    16201 atgataccgg gcaagaggaa tacggtgggc cggcccgatt gatgcgtcgg ggccactttg
    16261 attttacgga gatggctcac agaggcccgg agctcgcgga agtcatctgc gagcaggtgt
    16321 tgcgccgtga gtgccgtgag aatggcctga cgagttcccg gattgaggaa ataattgcag
    16381 tggttgacta cctcatcctt gctcagatgc tcccacacgg gttgaagccg cggaacaccg
    16441 atgcgcatgg aactcacggg caccaccagg tcgtgttcct ccctgaaaaa cagatcgccg
    16501 accaagacca acagcttgcc gaacagtttg cctgcgcggg catcgccgga tatgacgtag
    16561 agcgaggatt ctaatttctt ttcgctgtga ttgagcagtt tcaccaaagg ggaatccgac
    16621 atcatggcgg ccaacccagg cagcgacttg gcctcaaacc gctcgtggac aacggctttg
    16681 atgaaatccg acaaaaactc gtacaccgga ttgccgctga agccgggaat gagtcttaga
    16741 gaattgagca tcacattgag ccatacgtcg aggcgtttgc tcatcagcgt ggtgccgccg
    16801 gtggggcagg ctacgcgcac aaacttgcgc actttgggct ttttgtctct gatcagttcc
    16861 tgcactttcg ccagctcgcc gagcagcgcc ttctctttat tttcaaaagc tttgcggagc
    16921 gtttccgaca attctacgtc ttcagaaaaa cgagccagca cctccccgat caggccgccc
    16981 cgcgaatggc tcacgagatc gagcgtaatg ccttccggca gcgattccag caggtctctg
    17041 gtgtttttga gcgggctttc cgacaaggtt ttgtgttcaa atgccagcac ccggccttcg
    17101 taggcgccgt atattttttc ccaggtagtc cggtcctttt cggtgaagga tttgaaagcg
    17161 cccaccacat cggaggcggt gccgtgcagc agcagcaggt acggttcgga tggcgcggtt
    17221 ttaaaatcgg gcagttcgag cagttcaaaa tttttgccaa gctggtgcag tcctgttcgg
    17281 agcttgcttt ccattttttc ggcaattatt ttcaccgcca cttctccagc ccagttggtg
    17341 tagaaacgca tcaacttatg cttggcggcc tccgtcagca aactgcgggt gcccggctgc
    17401 gtttcaaatt gcgctggtat gacgatcaca ttgggatcgg tttccacgcc acgcgcctga
    17461 tagctgcgac gcaaattgtc ggccaagtcc tcggcccgga caaaccactg gctgtcgtcg
    17521 tccatgacga attcaaaaac atcgttttcg agcgcatcta tctcaataag cggctcctct
    17581 gccgagcgac gcatcgtcac ttcgtattct gctacttcta ccgtcggcaa gacgcgggct
    17641 gtggtggccg aggcgggggt cggcactccg agcggggcgc cggcaaattg gaatttcagg
    17701 cgattcatgt tgggcggttt tttattttga agtccaaata taccgatttt gtgaatacga
    17761 gccaaaagga tgacaatttt tacagggatt caaacggcgt gtccgacctt tgcccgcatg
    17821 agaaccatcg ccatcttact cctgctgagt acattcagcg tcggcattgc ccaaagccag
    17881 ccatacaaaa accctgccct gccggtggaa gcccgcgtgc aagacctcct cgcgcgcatg
    17941 acacccgaag aaaaattcat gcaaatgttc atgattcccg gcgaaacggg cgccgacagc
    18001 ctgctgtacc gggccggcat tttcgggttt caggtcagcg cggcggggca aagcgatcat
    18061 gccgccggtc agatgctgcg ctatgaaggc ggcagttcgg cacgggaaac agcgctgaaa
    18121 atcaatgaaa tgcagcgatt ttttatcaat aaaagtcgcc tcggcattcc catcattcct
    18181 ttcgatgagg cgctgcacgg attggtgcgt tcgggcgcta cggccttccc gcaggccatc
    18241 ggactggcgg cgacctggaa cacgccgctg atgcacgaag tagccggcac gattgcgacg
    18301 gagtgccgca cgcggggcat ccggcaggta ctctcgccgg tggtcaatgt ggcaaccgat
    18361 atgcgctggg gccgcaccga ggagacctac ggagaagacc cgtttttggt gtctgaaatg
    18421 ggcgtggcgt ttgtctcggc ctttgaaaaa caaggcatta tcacaacgcc caaacatttt
    18481 gtggccaatg tgggcgacgg cggtcgcgac agctatccca tacatgccaa cgagcgctcc
    18541 ctgcgcgaaa tacacctccc gcccttcgag gcatgtttcc ggcggggcgg cagtcgctcg
    18601 gtgatgacct cctacaattc ctacgacggc aggccctgca ccgccaatgc ctggctgctg
    18661 cgccgattgc tcaaagagga atggggcttt cccggcttcg tcatttcgga tgcgggtgca
    18721 acgggcggcg ccaatgtgct gcacttcacg gcccgcgact accccgacgc cggcgcgcag
    18781 tcggtcatcg ccggattgga tgtgattttc caaaccgaca tcgcgcatcg caagctgttt
    18841 tcaccacctt tttacgacgg tacgattccc gatacagtgc tgaacgaggc ggtgggccgc
    18901 atcctgcgca tgaagttcga gctgggtttg tttgaaaatc catacacaga accggaagag
    18961 gccgctcgcc tgaacggaac tgccgaacac cgggcgttag ccaaaaaagc ggcgctggaa
    19021 tccctagttt tgctcaaaaa tgaagggcag gcactcccca tttcacctcg cgtccgttcc
    19081 atcgcggtca tcgggccgga tgcagcggaa gcccggctgg gcggatacag cggtcccggc
    19141 aaccgcccgg tcagtatttt ggaaggcatc cgaaaaaggg cgggcgatgc ggtgaaaaca
    19201 ggctacgcca aaggttgcgc ccgcacggaa acaccctacc tcacagtgcc tgcggaggcg
    19261 ctgtcctgcg tcatggacgg tcaggagcag cagggacttc ggggcgcgta tttcgacaat
    19321 gtcacgctgg agggcgagcc ggttttcctg cgctgcgatc ctcagattca gtttcaatgg
    19381 acgctctttt cgcccgaccc ggagcggctg agctatggtt tttactccgt gcaatggacc
    19441 ggccaaatca ccgcgccgca atcgggccgc ttccgcatcg gcattgacgg caacgacggc
    19501 taacggctgt atctcgacgg caagttgttg atagacaatt ggataaaaaa gagtaggcaa
    19561 acccggctcg ccgaattcaa cttcgtcaaa ggccgcgctt acgacctgcg catcgagttt
    19621 tacgaaccca caggcaatgc ctggtttcgc ttggtttggg acctgagact gagcgatgcg
    19681 accgaaaaaa gcatccgcga ggcaacccga ttggctgccc gcagcgatct ggccgtggtg
    19741 gtggtcggca tcgaggaagg cgaatttctc gaccgcgccg acctgagtct gccgggccgt
    19801 caggaggaac tcatccgccg ggtagccgat accggtacgc cggtggtggt ggtcatcgtg
    19861 ggcggcagcg ccgtgaccat gagccgatgg atagaccggg cggcgggcat tctggccgtt
    19921 tggtaccctg gcgatgcggg cggagaggcg gtggccgaag cactgtgggg cgattacaat
    19981 cccgccgggc ggctgcccat taccttccca ctgctcgaag gacaactgcc tttggtatat
    20041 aaccacaagc ccaccggtcg cggcgacgac tacgtccacc acagcggaaa gcccttgttt
    20101 cccttcggtt tcggcctcag ttatacggcc tttcggtacg atggcatccg tatagatcgg
    20161 gaccgcatag cgccggggga ttccactttt gtgtctgtca gtattaccaa taccggcacg
    20221 cgcgcgggcg acgaggtcgt gcaattgtac ctgcgcgatg agttggcctc ggtggcgcgc
    20281 ccggtgatgg aactcaaggg ttttcagcgc ctgcacctgc tgcccggcga atcgcgggaa
    20341 gtgcgtttcc ccatcggccc tgaacaactg aggatgttgg acgagcagct ccgcccggtc
    20401 atcgaacccg gcgcgttcag cattctgatc ggtgcttctt cgcaggacat ccggcagaag
    20461 gcggtgctga tggtggggcg gtgaggcgcg caaccaaccc tcccttcaaa cgtccctcaa
    20521 gctgcctgaa aaacatcggc cttatttcac aatcatccca ttgccggctc cttccggcgc
    20581 actcacaaag gagagctttc cggcggcgtc ttcggccatc agaatcatgc cctgactcat
    20641 gacgccgcgc agctttcggg gcgccagatt ggccaccaat acgacccgtt ggcctaccac
    20701 tgcctccgga cggaaatgca gggcaatgcc gctgacgacg gtgcgctgct caaaaccgag
    20761 gtccacggtg agttggagca gtttgtcggc gcctttcacc ggttctgcgg cgatgatggt
    20821 ggcggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagccccac
    20881 cgcttcaggc tcctggctct gagcttcggg ctgtggcggc gttgttgtct gttcgggcag
    20941 catcagcgcg ctgagtttgt tcttctggcg ctctacgatt tcccaacggc ttttgtcgtt
    21001 gcgatccatg attttgggga acaatacttc gggttgcccc aatacatggc caggagtggc
    21061 aagcgcctgc ccctgtgcca tcgcttcaaa cgctgcattc aagtcaccgt tggatacagg
    21121 cgcgtggccc agcaaacggc ggatgcgcgg cgatgtaaac ggcaggaacg gctccgaaag
    21181 cagggccaac aattccgaaa tctggataca aacgtagagg cactctgcaa tggccgggtg
    21241 ctgcgggtct tgtttccaaa gcgcccaggg cgaaacatcc tgcaaatagg tattgccgaa
    21301 agacgacagt tccatgagca tctgcgcggc gtttttgaag ctgaacgcct ccaattcggc
    21361 agctatgttt ttggccaatt gctgtgctgc cgccagttta tcggcatcgg cgccggtggg
    21421 ttgcggcact gccccgttga agtatttgtg cgaaagcacg atgacgcggt tgacaaaatt
    21481 gccgaggttg tcggccagtt ctttgtcgtg gaaatccaca aacaaatccc atttgaaatc
    21541 gctgtcgccg ttttcgggca tgttgcgcgt cagcgaccaa cgcagggcgt cttctgcatt
    21601 cgggaagtct ttaaactcct ccagatactc gtgctgctct aagccccagc ccctgctttt
    21661 gctgaacttt tcgccctcaa aattgagaaa ctgattggcg ggcacgttca ccggcagatt
    21721 gtaaccgccg taagcgtaca gcatggccgg gaagatgatg cagtgaaaaa cgatgttgtc
    21781 tttgccgatg aaatggatga gcttagtatc gggattgcgc cagtagtctt cccagttttt
    21841 gccgttttcg agcgcccatt gtttggtgct cgaaatgtac ccgatgggtg cgtcgaacca
    21901 gacgtacagc acttttccgg cggcttcggg cagcggcacg ggaatgcccc agtctaagtc
    21961 gcgggtgatg gcgcggggtt gcaggccctc gttgagccag ctcatgcact gaccaatgac
    22021 gggctttttc cactcgcggg catcgtggtg ttgttggccg tcgagttcgc cggtttcgat
    22081 ccagcgacgg agccattcgc cgtgcttgtc gagcttgaag taccagtgtt gagtggctct
    22141 cagcaccggc gttttgttgc tgagtgtgga aatcgggtca atcaattctg tgggagaaag
    22201 gtcggagccg cacttttcgc attggtcgcc atacgcctcg gtgtggccgc atttggggca
    22261 ggtgcctttg atgtagcggt cggcgaggaa ttgcttgaag tcttcgtcat agtattgctc
    22321 cgaactgcgc acttcaaact cgtcgccttt ttcgtgcaaa cgggtgaaaa aatcctgcgc
    22381 cgtggcatgg tgcagggctt cgctggtgcg gtggtagtaa tcgaaagaaa tgccgagccg
    22441 ggcaaaggta tctttgttga gcgcgtggta tttgtctatg atttcctgcg ggctgatgcc
    22501 ttcttttttg gcccggatgg tgatggccgc gccgtgttcg tcggaaccgc aaacccagac
    22561 tacgtctttg cccatgaggc gcagataacg cacataaata tcggcgggca aataagcgcc
    22621 ggccaagtgg ccgaggtgca gcgccccatt ggcatacggc agggcagagg taatcaaata
    22681 tctgttgtcg ggcattttgt aattgatttt ggaggcgcaa aggtagcgag aaatcggtag
    22741 tgagcaaagc agacggtatg ccgctcacca ttttcgggta tttaggcccc gatgtttcaa
    22801 atccctgaat atggtgttga ttgccccctg ctgccgaggg taactttgca acatccagaa
    22861 cgcctgaagt tccatcacat ctcaaaaccg aacagatatg aaaaagaaaa catttacgct
    22921 cctgatcgca atttgtctcg cgcatttcgc atcggcacaa aatgccccta cccacgaaca
    22981 ggcttacggc tacattggca gcaacggcaa tatgagggtc atcaaacctg catttttggc
    23041 cggacagtat tttgccggcg gcttgatgaa caacgaggct accatcattc gcgtgaacag
    23101 ggcggggcag gccctttcgc atcgtcaact gaatctgccc ggcgccaccc aatcggagat
    23161 tacagatgca gccttagtca gtgcaacgct gttggcagta gccggaacct gcaccgggtg
    23221 catgagcgcc cccgatcgaa aaggcgtatt cctcatgctg ttcgatcagc atctcaacga
    23281 agcagccacg ctcttgctgg agccgaatga acaacaggca catttcgaaa atgcccgctt
    23341 ggcggtgggc aacaacaccc tatacctggc gttcaacgat ggcttttttg gcggaagcca
    23401 ccacattcgg gcttatgatc tgaatctgaa tcaattatgg gcatcgtttc aacatctggc
    23461 ttttatagaa tctccggtta agctattttt tcagggaaac gaactccgcc tgattgccaa
    23521 cgagtggaca ggaaacacca acacaggcac aaggctcatc cggctgaacg catctaccgg
    23581 agtgccggcg gggcactatg cctatgctgc ttatgccata gatgcagcgg agttgcccga
    23641 cggaaattat gcgattgcgg cagcaggcac cttctacccc ggagatcaga aaaacaagat
    23701 ttttgtggta tctcccctca gcggcgaggt gctggattcc accttagtgg gccatcatgg
    23761 caattcgcgc atcacggctt ttgatgcttt gccgaacggg caattgatgg cggctttgaa
    23821 cgaatttgct ttttttgagg acacgctcaa attcatccga ttcgatcctg ccgatttgga
    23881 agcgcctgtc tctgaacgca ttatatgggg tggtcgaggc gccaaagaac ggcaggtata
    23941 tgattttttg cctttgaacc aagatggaag cagctacctt tcagtggggt ggcgaagtga
    24001 tgtaagtaca cggggcatgt tgctggcggc ccttcccgga ggctttacat tgcaagcgcc
    24061 ggcgcagtgg aacaacgaca tctgcggaag cgacctgctg caatcgccct accaacaaca
    24121 ttaccccagc gtacttccaa ttattcatga aaacgttgtt tatcaggcaa atgcaattct
    24181 ctataatggg caggtacgag atttgagcat ggatatttat gtgcctttgg atttgtacag
    24241 cacggggcaa tctactcaaa aaaggcccct gctgatgttc gttcacggcg gtggttttat
    24301 aggtggtcat aaagacgttt tttcttccta cgcattggct ttcgccgaaa cgggctatgt
    24361 gacggcctcc atcaactacc ggctcggcgt agcggatggg gtcaatagtt tatcacaatt
    24421 ttgcgaccat accgaagcgg tttttgcggc catgtaccgt gcaactcagg atacccgtcg
    24481 tgccattcaa tttttgtacg accatgcaga cgactaccac atagaccgga acaagatttt
    24541 tgtgctggga cacagtgccg gcgccatcaa tgtgctcaat tcaggcttgt tggatgccga
    24601 cgaactgccc tatccttttg agtctcagtt gggacctttg cctcccaaac ctcccgtagc
    24661 ggcatacctg ccctgggcgg gggccattgc aagcctcaat atgatagata ccgatgagga
    24721 tacgcctgtg atgtttattc acggcacctg cgacccgttg gtaccctatg gcgaaggcaa
    24781 tctcatttgc ccggacttgc ccttcgggtt cggctcgcgg gccattgccg cgcgaaaaaa
    24841 agaattgtgc cacgactacc tgctgttggg catcgaatat ggagatcatg gcggcggcgg
    24901 cagcgacctt gacatacttt accggatcat tgattggctc aaaaaccaga acatttgcgg
    24961 aaccccgaca caggaatgtg aggtcatcag cgcggccaat cccggcaact gccccccgca
    25021 aacgatgtgc cctgacacgg agccttgttc ggagcctacc tctgcgctca atgttccgga
    25081 ggcgcccgtt gtggagattt ttcccaatcc ggccacagaa cgggtgacga tctctttatc
    25141 taatggtgct gtttttaaag gaagcttagc catttacaac actgacggac aacgcattag
    25201 atcatatcct cccaatgaga acacccgcat tgatctggat atttccgact tgccggccgg
    25261 cgtgtacttg gttgagatgt tcgttgccgg aaatacgatg agacataaat tgatcatact
    25321 ctaaaatcca acccgatcat gaaaacactc tatacgctgt tttttggcct ttattctttc
    25381 tgcgctgccg cgcaatactg tggcccgctg catgtgcatc ccggcgctac cggcgcgggt
    25441 accggcgaat cctggcagga cgcattcgtt caccttcagg atgccctcga tgcggcccaa
    25501 cccggcgccc agatttgggt agctgccggc gtctatctgc cgcaaaagga tacgaccggc
    25561 aatgccacgc ctgccaatga tcgctccaaa acctttttca tcaataaaga cattcagcta
    25621 tatggcggtt ttgcaggcgc cgaacaggag ctttctcaaa gagacccggc agccaatccc
    25681 accatactca gcggcgacct cggcactacg ggcatcgcta ccgacaatgc ctaccatgtg
    25741 ctgtggcttg cgctggttag cccgctttgc atcattgacg ggtttaccat cacacgcggc
    25801 aatgccaatg cctcagacgt caacggcacg ggcggaggta ttttgctccg gcatcagccg
    25861 ctgcaatacg ccgctccggt gatccaaaat tgcatcatcg agcacaacca tgccactacc
    25921 ggcggcgggt tttacacagc taccgacagt tgggccggcc cctctgtgag cattgaacat
    25981 tgcaaattcc gaaacaatac tgccagctcc ggcgggggca tttattttca gcagagtatg
    26041 gttgcagaag tgggcctcca actgacgaac gtggagtttt cagatcagag cattggccag
    26101 tacgggggcg gattgtatgc caacggtcat tttacagccc gcgattgtcg ttttgaaaac
    26161 aacagctcca accggggcgc cggcgcaaat ctggaaggtg gaaccgccat cttggaaaat
    26221 tgtgccttca tcggcaatac ctcgctgcgc aatggcgggg ggctgcgcat ggagcgaact
    26281 acggccaccg tggtgaattg ccttttcctt ggcaacgagg ccaatatttt gaatgaatct
    26341 tcaacaacca gcaatgccgg cggggggatg ttttaccggg ggtccaacac ctcctttgca
    26401 aagctggcca actgtgtgtt ttcgggcaac aaaggccaga atggggcggg ttttgctcaa
    26461 acggctaccg tgctcaacac ctcttctgcc gaattggaca actgtacctt tgccggcaac
    26521 aacggagcga gcgctgtggc gcgtttgcag ggcaatctca ccatagaaaa ttccatcatt
    26581 ttcggcaatg aaggcacggc gctcaccggt ggcacggcgc gtagttgcat catcgaagga
    26641 gggtatgctg gcacagacaa tgtgagtgtt gatcctctgt ttgttgcatt gccctcattt
    26701 ttgctcgcac ccactgctgc cggaaatctg aaattgcagt ccggctctcc ggccattgat
    26761 cagggcgaca acgctctgac gccgacgggc ttcatcaccg acgccgacgg caaccatcga
    26821 atgataaatt gcaccataga cagaggcgct tttgagtttg gaacctccgg cgcttccgac
    26881 cggatttgct atgcagatgc agatggagac ggcctgggca atgcagccat gccgcgctta
    26941 gcgtgcaaca cctgcccggg aggttttgta tcgaacaacg gcgactgcga cgacagcaat
    27001 tccgccatca cccatacgct gccgcctcct gctgccatcc aactcaccac tgaattttgt
    27061 cggggcgaac cctccatcac cctgcgcagc atccaatctg ccacaccggg gcggttggtc
    27121 tgggtactgg tgcaagcccc gccgggcagc cgttttgagg ggcaggaacc gcttgaattt
    27181 caccccggag ccgtcaacgc cgagttttcg acaggaaccg acaacctgct gatgtgcagt
    27241 acgccttcct atcaaaatac atcggtaaac ggtgtctgga tgtttgaaac atattacgaa
    27301 acggctgccg gttgccgcag tgcagtgcag cagggattca gcattacagt acgccagaca
    27361 agtaccaccc aactttccac caccgcctgc aacagttaca ccttcaacgg gc
//
LOCUS       DAORLF010000010        26329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1017_length_26329_cov_2.298813, whole genome shotgun sequence.
ACCESSION   DAORLF010000010 DAORLF010000000
VERSION     DAORLF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 26329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26329
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_26329_cov_2.298813"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..745)
                     /locus_tag="PK198_01865"
     CDS             complement(113..745)
                     /locus_tag="PK198_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37506.1"
                     /translation="MIRVSIVEDQPDILNSLVRRIDRTEGMICVSQFSNAEDALERIP
                     AQHPDLVLMDIGLPKMDGVECMIRIKLQAPEVDFLMFTVFDADDYVFDALKAGAVGYV
                     LKNEGAAGVVKAIEEYALGGAPMSREIARKVLGSFHIQYRRNTSGFEELTPQQLLILE
                     QLSQGLLNKEIGDRLGISEGTVKQHNYAIYKKLQVNNRTEAVQKYLNNRP"
     gene            complement(839..>1080)
                     /locus_tag="PK198_01870"
     CDS             complement(839..>1080)
                     /locus_tag="PK198_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37507.1"
                     /translation="ILEMFRVDKSDRAYQFWQREPKSIAIVNEQMFYRAVNYIHRNPV
                     AKRWILAPAPELYKYSSGYNNTHPGYWEFLSELWG"
     assembly_gap    1081..1092
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1093..1360)
                     /locus_tag="PK198_01875"
     CDS             complement(<1093..1360)
                     /locus_tag="PK198_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF37508.1"
                     /translation="MHVQYPELYFTTASILGWKSLLFDDAMKDIIINSFRFLVREKRC
                     AIYAFVIMPNHIHVVWSIEAPYTLSQVQAALTSFTAHAFPKKLRA"
     assembly_gap    1488..1531
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1532..>3655)
                     /locus_tag="PK198_01880"
     CDS             complement(<1532..>3655)
                     /locus_tag="PK198_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF37509.1"
                     /translation="IGGAYLPSENSLLACTKNEGLLLFDLARRQWKGQVIPQAGQTSS
                     LLNAAPRGLLISREGQVWVGYESGGLSYSRIVQRGFEELMSENPAHIRAAVQMPDGAL
                     YMATNDGRMLVYKDGKTEQRPALARPLIQLSCDENGGLWGLSQQGLYRMKKGAADWET
                     CLEVELGFVAVFTGIPGRKLLTTQDGIFDVKERAGKFSLIPAPEFSSHKGYFFSEIFP
                     VGNQTVLIPFESKELWVAKLEGQSLRIVNRMDIGGDTQGACITPGGDSIWIASNTGLL
                     LYTDGHLKRVLDSGTDLNGADVTSVFRTPSGYLWLGTLRGLYRYHPASRELRGFFEQD
                     GLSGEQFAYWAALQSNDGRIFLGHSKGMLAFRPEAVQPTSSRPRAHIEALWVNNLLFE
                     TPKVIAETDTLRLGYRENTLALELTAIGYHQSEGSTLAYRLRGYDNNWTTVANGGFAR
                     FTKIPPGQYTLEVLPRNANGETGELRSMAVWIQPPFWQTWWFKIGSVLLAILLVGGSV
                     GLYYRGVLRRQRALLERQEALHAERNRIAKELHDDMGGSLSSIRFLSEDLLLDDDAPE
                     LRRISQLAEKTLENMRVIIWAMDSEKNTLQDLASRLRSLAGELLSDNKIAAVLDIPTA
                     GLENVVLGGERRRNIYLIAKEALHNVVKHARATEVRVSLQLQDGSIILTIKDNGQGLP
                     ENAGASEGYGLGSMQARAKAIDGHLE"
     assembly_gap    3656..3734
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3735..4459)
                     /locus_tag="PK198_01885"
     CDS             complement(<3735..4459)
                     /locus_tag="PK198_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37510.1"
                     /translation="MLFGSVARSTCSVYVVFVLSWMLLAVAGATAQVPAWQFEHLGPE
                     QGLSNNTANYFLSQDSRGFIWIGSLNGFYRFDGLRLRHYLIPKNEEDSYTPDQIIQSS
                     FWEDGAGNLWFSTYNSLHCLRRADGRMLNFRAPDGADRSYHVFHIEKKTGRLWLRAGD
                     QVWTFQPENGRWAAQFATQGVRFAVRADNKGVPQHIIACAWLKSPVSVHSAAGSDTWI
                     SENYLSPEMQIRSAYGAIAVRGT"
     gene            complement(4579..5451)
                     /locus_tag="PK198_01890"
     CDS             complement(4579..5451)
                     /locus_tag="PK198_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37511.1"
                     /translation="MAKQTLNLNRVDSTTTSNATPGSGNPALLLKTGSIAYKIDTGNS
                     DWKKMLDPQKASNAPANQPLIGLQVKFNKGTVKAPEITQLCFDINPSDGTSYFFPGAC
                     SIALEDSAAYDILEKPDSADKTILYAELSPTTSAPAAAGTNLYKQLDGYEDIDFVFFP
                     LDQLSAMTDLYKCLIISGSKISPGRKLSHRPQALTADKIYEEDYFSLKIEGSEPTSGW
                     GSQNATGPQEAGAAMLPGHPCPPDWQLFTAMMGAVYSKVSNSGVVLTPTVRAAIRRSF
                     NTQVVNNAVQTSHA"
     gene            complement(5749..6183)
                     /locus_tag="PK198_01895"
     CDS             complement(5749..6183)
                     /locus_tag="PK198_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37512.1"
                     /translation="MNIFKFFSIAAVAFTALFFSATPAASAQQTREHILLIQMNVEPQ
                     AAAYIKFDGVDGECSVREGRNTVGRNTRTGDLLVMVVRNGKLVEFGTQNAAGAFKAIS
                     PNASPCNSTIHCTTLNPPKCFTLPGGACVCVCGPWITAAGSN"
     gene            6395..>6669
                     /locus_tag="PK198_01900"
     CDS             6395..>6669
                     /locus_tag="PK198_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37513.1"
                     /translation="MYNPAKTFIQRLSDFQHDFSGDPRWELVFSNGKRKWFYIVVSYY
                     NGIYYISERIGDHAPIQVNEQGEMTANAHYGGARLDEKKWQTLFSLA"
     assembly_gap    6670..6725
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6944..7780
                     /locus_tag="PK198_01905"
     CDS             6944..7780
                     /locus_tag="PK198_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF37514.1"
                     /translation="MKTSFLLATALLACMYAGAQSRGDLTLMSFNIRFDNPGDGDNRW
                     DKRRDDVAQTMLFHQAQVCGLQEALHSQIQDLLQRMPGYAFVGVGRDDGQLKGEFSPI
                     LYDTRRLQLLDSGTFWLSETPEKVSKGWDAALPRVATWAKLRDKTARKVFFVVNTHFD
                     HIGQEARKNSAALIVQRVQQLAGSSRAFVMGDFNARPDEAPIAVLLQAYRDCKALSQM
                     PHFGPEATFNGFGPKEQEGMRIDYIFCNDPSVKVLQHATLSGTWGGRFASDHHAVMAR
                     VR"
     gene            complement(7866..8369)
                     /locus_tag="PK198_01910"
     CDS             complement(7866..8369)
                     /locus_tag="PK198_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37515.1"
                     /translation="MATDIILFSERQYFRQWWLWGLLLMPFGLILYGLYVQLVQGIPF
                     GNNPGSNEMLIGVAVFLLLMLVAFYFMSLQVELSRAGIRYRFFPFFGSRFIAWEEVAE
                     AEVRQYQPLKEYGGWGIKYGSGGTAYNVSGNMGLQLVLHKNNKRILLGTQQPEALRLA
                     LQKMRPH"
     gene            complement(8423..>11154)
                     /locus_tag="PK198_01915"
     CDS             complement(8423..>11154)
                     /locus_tag="PK198_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRF37516.1"
                     /translation="ATALPPTEMAQGAELSDYFSDYMNGMAQRETPARLPEAQISHIR
                     SECVRLFQEMGFGAYARIDGFAYSDGRILIREANNTSPLLPSSLLFHQAAEIGLNPTQ
                     LLNFIVRASLQERRAEHPERSAYADLLQGLDAALDALRHTATTKRNIAIAFSGADEDV
                     ESARNVFEKLAASGKYAPFPVLLDDRQGRLLMYKIPTGFLFHKRFEDLRSLLDHWQAS
                     PVLEAIRAECAGLLRKYTAAGALVSEPKLLAQEELSLHADAVWPIVQNAPETLSKLLK
                     SVEQADLPHTGSDAVSSAIAADKYRSLQTLKRNGLSVPDQIVAHQTEYEASAEEFYRR
                     VESMLSYPFTARPLDGSHAPKTLSERRHLEAYTRIVFRPQGMESEEARRVLRLRMTES
                     IPRRPQILFESLVAQQNALQLLEVRGGLLTSFKPDGTPVYEVLELSEGALGSAVPGGQ
                     APNTALITPARLGGSTGGDYHKIAGQVKGDLERAARITGVQGYALLSAYVRIFEHGRA
                     ETILTDIHPQPALTPASCIFRQAAFLQFKPYDVLDQVLRFAFDRKAPVSVAQPVSATA
                     APATTELPMPAITDEPEAQESYFDPAVRPQQVSYDGEPGAWRDTRKQTEPHFEGSSGR
                     VLTYYLSEMAQFLRSGIFLRNLAALAAFAVLGIFLLQLVLRFYTRHNESVQVHDYVGM
                     SLNEAIAKARSRNFRIVVTDSIFKVDARPNMVLEQSPAPLSRVKERRRIYLTVTKATP
                     DMVALPDMVGSYNYDQYSRKLAWVNVRSKVRERKFDEKLEENTILHFFYEGKQYTERD
                     VRNGLKVPMGATLEFVVTERLTGMVAVPDLVCKTYEEALFIITSFNLNIGTIHGDGPG
                     SDQFYIWKQEPAFAPGVTLSAGRQIDLYLSPEKPAGCGASEPALEQQDDGGEE"
     assembly_gap    11155..11167
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11168..>11998)
                     /locus_tag="PK198_01920"
     CDS             complement(<11168..>11998)
                     /locus_tag="PK198_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRF37517.1"
                     /translation="RAYPPAEYLPDSPNGFQVYMESLGELAQEDLNALVARVGRRIRL
                     DELPGLIDFAFLAMHGEYGADGRLQRQLDDLGIPYSGSGIRAAEIGSDKVLQREWLPA
                     HGFYTPKSFVIPREQWIADNTAADFYRQAADTLGFPLVLRPATQGSSIGVSVVDEPGY
                     EEGFRDAVNRAFFREIVPVATWRSRSKFEKTDYIRLLTDLRDGLGFPVTVAYEGGQAL
                     VYHPESLLAHLDQLAHEALPGSEAVFVIESTLSEGAVIVEELIDSHEFSCIVVRNEDG
                     S"
     assembly_gap    11999..12074
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12325..12525
                     /locus_tag="PK198_01925"
     CDS             12325..12525
                     /locus_tag="PK198_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37518.1"
                     /translation="MSTYDQLIEKGRAEGREEGIEKGIEKGKTEVVLNADTNGLSISL
                     IANITGLEEAAILRILQEHGKA"
     gene            12585..>13489
                     /gene="pgi"
                     /locus_tag="PK198_01930"
     CDS             12585..>13489
                     /gene="pgi"
                     /locus_tag="PK198_01930"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537059.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRF37519.1"
                     /translation="MVLPRINPVETTAWQKLLSHYEAIKLVQMRDLFAADAQRFDTFS
                     LRFEDLLADYSKNILTTETLDLLLQLARETQLPQAIAQMFAGETINETENRAVLHTAL
                     RNRSGRPVLVNGADVMPAVHAVLTSMEQFCAQIHSGDWRGYSGERITDIVNIGIGGSD
                     LGAVMVTEALRPYWAEGIRAHFVSNVDGTHIAETLRRVRPESTLFIISSKTFTTQETM
                     ANAYSARQWFLEAARDEAHVAKHFVAVSTNAGAVQKFGIDPANMFEFWDWVGGRYSLT
                     SAIGLPIACTIGFDNFRELLEGFHA"
     assembly_gap    13490..13577
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13578..14259
                     /gene="pgi"
                     /locus_tag="PK198_01935"
     CDS             <13578..14259
                     /gene="pgi"
                     /locus_tag="PK198_01935"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768321.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRF37520.1"
                     /translation="FFGAETEAILPYDQYLHRFPAYFQQGNMESNGKNTDRNGQLVGY
                     QTGPILWGEPGTNGQHAFYQLIHQGTKLIPCDFIAPAISHKPIGEHHKLLMSNFFAQT
                     EALMKGKTAEEVRAELAAAGKSEQEIEALLPFKVFPGNRPSNSILLQQITPRSLGILI
                     ALYEHKIFAQGIIWNIFSFDQWGVELGKQLANNILPELQNDSLVSSHDASTNGLINAW
                     KEMRSAQG"
     gene            complement(14446..15630)
                     /locus_tag="PK198_01940"
     CDS             complement(14446..15630)
                     /locus_tag="PK198_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF37521.1"
                     /translation="MTKLLHFRLSALQFMQYFLWGAWFVTLGSYILAHLGDDGALVGT
                     AFGATAIASSLTPFLMGVLADRHIPSQRLLSILHFLGGLMLLGASQMKSFGSFYAFFL
                     PYTLLYAPTFSLNAALCFRHLSDPGKEYPRVRVWGSVGWIVAGIMVSAFALEESATPM
                     LISALMSFVQSLYCLSLPHTPPLGAQQPGATAQKWFNPEIKALFRNRSFSVLVICLAL
                     STIPGGFYYSFVNPYLNDIGVGHAAAKMTLGQVSEMLLMLFLPWFTLRFPLRYVLLSG
                     FIAWGARYAMLALGGTGLMYGAIALHGVCFIFTALAAQIFIDSMTPPSLKSTVQGFIT
                     FLTMGLGTLIGSHLAGWVVKHFTIGGVRHWEAIWWTPAVLGFVIAAVFGLFFREHPAK
                     QG"
     gene            complement(15649..>17128)
                     /locus_tag="PK198_01945"
     CDS             complement(15649..>17128)
                     /locus_tag="PK198_01945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF37522.1"
                     /translation="KFMVEKLKTATSMCRMPGVNICRALIRYVISERQRYSKTIPPHL
                     AFPLPTITNDAMKHPAPEAVSKPCAIQSALSFLYAPVAETAGLCRRSATARSLENEVA
                     LPLPGWTARVFSSLEQTPEDWDLAAPSDDIFLQRNYLTVMEQCAPEGMAFAYVVFYKQ
                     ARPVGLAYAQLLDLRIKKSLESGLQAERGWFKQIKSVLAGLGRFQLLVCGNMLLTGAH
                     GFHFSVEGLREDQTARLLDSAMEAVSERFRCCGRKVDLAMVKDISPCCKGVSEQLSRM
                     RFSEFSFQPNMILHIQPSWQRFDDYLEAMSSKYRVRARRAFKKMDGITRRELTEKMVV
                     LEEAAMYELYCEVASHADFNAFDLHPGYFTALKQAFPQHFRVFGYYQNDELVGFCTTM
                     RNGHSMEAHFLGFRNALNASHQLYLNMLYDMAKTAIESGASELVFARTAMEIKSSVGA
                     EPQQLDCRLRHYHGPSNRLMPFFIRLLEPSVQWVQRHPFRAE"
     assembly_gap    17129..17151
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17581..17940
                     /locus_tag="PK198_01950"
     CDS             17581..17940
                     /locus_tag="PK198_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRF37523.1"
                     /translation="MVLHFQLVGTLAGAVGTAVQFQMPQGWDWIMLLGMGLLTQLGQL
                     QLTKALQIEQLGIATSLNFLGVLYAVFFGVLFFQEHLTPGNILGMILVAAGVVANVVT
                     GKRAEVIATTNVVKPYN"
     gene            18012..18968
                     /locus_tag="PK198_01955"
     CDS             18012..18968
                     /locus_tag="PK198_01955"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRF37524.1"
                     /translation="MNTLIGTDISAARHFLEAGHPIGIPTETVYGLAASALSPAAVAR
                     IFEVKTRPTFDPLIVHVPDAAAAQNYALHWPETARRLADAFWPGPLTLLLEKRDIVPD
                     LVTAGLPRVGLRVPSHPLALQLLQTLSFPLAAPSANPFGYISPTTAQHVFDQLQSLIP
                     YILDGGPCRVGVESTIIGFEDGKTVVYRLGGLSIEQLEQVAGPLTLQLHQSSNPAAPG
                     MLTTHYAPRKPLYLGPIDELKERFPQLRAGLLTLCPLPQKSADILCISLSSEGDLNEA
                     AQHLFAAMRLLDQSDVDAIFAEPMPETGLGRAINDRLKRAAV"
     gene            19035..19679
                     /locus_tag="PK198_01960"
     CDS             19035..19679
                     /locus_tag="PK198_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37525.1"
                     /translation="MKCPNCQTSIPIQNINVQTDIGKCDACSSIFKVSDIFTDVNPRF
                     NINHAPAGAWFHQNFNQTTIGATTRSPMAFFLVPFMLVWSGGSLGGIYGSQIVSGEFD
                     LFMSLFGIPFFIGSVVFWSITLMAIWGKVELTFNRQGGTIFTGVGKIGITKRFQWNEV
                     ARIEESISQYKYPGGHGAQIVFEGKNRHTFGSGLNEQRRYYLLQALKTMHAQGR"
     gene            19663..>20138
                     /locus_tag="PK198_01965"
     CDS             19663..>20138
                     /locus_tag="PK198_01965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF37526.1"
                     /translation="MRKDGKDKNSSKFLSFAAMNNSFLTVVSVLHTPGDMRVLPEFMR
                     GLWPVLQGHFADFEIILVNNCTELSPDEYLRPLDPELKQNIYVLQLSARVNRNHAVVA
                     GLDRANGDYVVVFEPGFYHKPELILDLFAKTREHFDIVYLRGRSRPISLKFRPFYKA"
     assembly_gap    20139..20205
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20206..>20541
                     /locus_tag="PK198_01970"
     CDS             <20206..>20541
                     /locus_tag="PK198_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37527.1"
                     /translation="IISRRALNSLLRLRENLRYMKAIYSIVGYNTAALDTDQKPEWDE
                     EDSFNERFGTSLVAITSYTTFLRSLLLWIFMFSLTFLIAVIVNALKVKFTGVDLLGNV
                     GEPFSGWTF"
     assembly_gap    20542..20557
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20875..>23125
                     /locus_tag="PK198_01975"
     CDS             20875..>23125
                     /locus_tag="PK198_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF37528.1"
                     /translation="MKNQRTLSLLLFFCIAVATSLPAQKKTQTPVTPTPAPAAAEFNA
                     GLYKGLKWRNIGPFRGGRSVAVSGVPGDENTYYAGYTGGGLWKTDDAGLTWRPIADGA
                     LQTGSVGDIAVAPSDPNVVYVGMGEHAPRGVMTSYGDGIYKSTDAGLSWKQIGLEKTR
                     HISDVVVHPGNPDVLWVAAQGALYGPSEDRGVYKSTDGGQSWKKVLYVDENSGASSLS
                     VDANNPRILYAATWQHRRLPWKVESGGAGCSIWKSTDGGDTWAKINEGLPKEMGKIGV
                     SVSPANSNRVYAIVEAEKSKAGLYRSDNGGKSWQLMSNDQNITSRSWYYMEVFADPQN
                     ADIVYVLNAPMMRSIDGGKTFSNISVGHGDTHDLWINPKNNQNMILGDDGGAEISFNA
                     GRSWSSLNNQPTAQFYRVNADKQFPYKIYTGQQDNTSVIIASRNNSFGITDKDWTTGP
                     GCECAWVAFDPDKPETLYGGCYQGYIDALDTRTGESKDIMAYPTLNLAIQPKDMKYRF
                     NWNAPIVASPHNANVIYHCANVVLRSADGGLRWEVISPDLTRNEAAKQGDGGGPITNE
                     GAGGENYNTIYYFIESPIEAGVLYTGSDCGLVHVSRDNGKSWLNVTPAGLPECSIHSI
                     EVSPHDKGTVYIAGTRYKFNDFASYTYKSTDYGKTWTRISEGIRTDDFLKVIREDPKT
                     KGLLYGGAERGFYVSFNGGQRWNQLQLNLPVVPVTDLIVRDNDLVASTAGRALWILDD
                     LSPLQQSAGK"
     assembly_gap    23126..23221
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23222..>23953
                     /locus_tag="PK198_01980"
     CDS             <23222..>23953
                     /locus_tag="PK198_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF37529.1"
                     /translation="FIPGIGKNPDNGVILSYFLPENADSNEVKLEILDAAGKVIRSFS
                     SKKDEAFKPYPGGPRPSPTLPAMKGLHRFAWDFRTGGLPDIPGVYVYADYTGHRVAPG
                     KYSARMSYKGQTSTADFEIAPDPRLQTAPQDWAEQQQTIARVEGHISDMHECVNALRK
                     VKKQVEHFNESFKNAAGDNEKAMLANGEQLLKKMAEWEANIVETRQRNFQDVINWPSK
                     LNAEFFDLRGKLDVHDPRVTQGVRD"
     assembly_gap    23954..24001
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24108..>25867
                     /locus_tag="PK198_01985"
     CDS             24108..>25867
                     /locus_tag="PK198_01985"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRF37530.1"
                     /translation="MRFSLFQLADAMQGRVIGQAPAGAVVEHLITDSRHIAFAPTALF
                     FALRGPGRDGHAFIADAYRQGLRYFVVARLPEVGSLPEACFIQVADTLQALQMLAAWR
                     RSLAAIPVIGITGSNGKTIVKEWLFQLLHPDRHVLRSPRSYNSQTGVPLSVWQLKPEH
                     ELALLEAGISRMGEMAKLAPIIQPTLGLFTTLGDAHSEGFPDAETKLREKLMLFQSAQ
                     LIFYNADDARVDAAIRALGKPLFCWGSAAHADLQLLRASSSDDRRSTRIEVRFEGRER
                     SIDIPFSDQASIHNAMLCWCVLLHLGLPDELIRERMSRLEPVEMRLEMREAVNACTLI
                     NDSYNSDLNALSIALDFMARQASGRRRTLVLSDILESSRAPETLYAQVADLICEKGVE
                     RLIGIGGQVQRLQGKLPDGIATVFYNDADAFLQQHDMETFSREIILLKGARRFGFERI
                     ADRLALKVHQTVLEIHLAALTHNLRQFQQLLAPGVRTIAMVKAAAYGSGSVEVAQLLE
                     LQRVDYLAVAYADEGVELRRGGIGLPILVLNPEEAVFDMMLRYRLEPELYSLRLLRNF
                     ARFCANRQEPVPVHLKVETG"
     assembly_gap    25868..26021
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26022..>26329
                     /locus_tag="PK198_01990"
     CDS             <26022..>26329
                     /locus_tag="PK198_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRF37531.1"
                     /translation="ESAWQRISAGLGYRPLRHILNTNGIARFPQYQMDMVRLGIGLYG
                     IPTENLDLQPVLCLKAAISQVRTLREGDTVGYGRRGAGQAGMRIATISIGYADGLLRA
                     "
BASE COUNT         5808 a         7070 c         7041 g         5638 t
ORIGIN      
        1 tgcccgaaag cggtgtgtct ttttttgcta ttattctgat gcaaaaatac ctgcctgggc
       61 aagcccttgc aatataactt tggttacaga ttgaacaggg gtttaaggat tattatggcc
      121 tgttgttcag atacttctgc acggcctccg tgcggttgtt cacctggagc tttttataaa
      181 tggcatagtt gtgctgtttc accgtgccct ccgaaatgcc gaggcggtcg cctatttctt
      241 tattgaggag gccttgagaa agttgttcca atatcagcaa ttgctgcggc gtcagttctt
      301 caaagccgga tgtattgcgc cgatactgaa tgtgaaaact gcccagcact ttgcgggcta
      361 tttcgcgact catcggcgcg ccgcccagcg catattcttc tatggccttc accacgccgg
      421 cagcaccttc gtttttcaac acatacccta cagcgccggc tttcagggcg tcaaatacat
      481 aatcgtcggc atcgaatacg gtgaacatga gaaagtccac ctccggggct tgcagtttga
      541 tgcgaatcat gcactctacg ccatccattt tgggcaggcc aatgtccatg agcaccaaat
      601 cgggatgctg tgcgggtatg cgttccagcg cgtcttcggc attgctgaac tgactcacgc
      661 agatcattcc ctccgtgcgg tctatgcgac gtacgaggct attgagtatg tcgggttggt
      721 cttctacgat gcttactctt atcatagtgt cagtgtatga ggtgctgtgt tggtgtctca
      781 cgctgtgtcg gtgtctcacc gacacaggta tgtcttccgg cgtcctcgcc ggctcaattc
      841 atccccaaag ttcgctcaaa aattcccagt acccaggatg tgtattattg taccccgacg
      901 agtatttata caactcgggc gctggggcca gtatccagcg tttggccacc gggttgcggt
      961 gaatatagtt gactgcgcga taaaacattt gctcgtttac gatggctatg gatttaggct
     1021 ccctttgcca aaactggtac gcccggtctg atttatctac acgaaacatt tccagtatgt
     1081 nnnnnnnnnn nnctgccctc agtttttttg gaaaagcgtg tgccgtaaag gatgtaagag
     1141 ccgcctgtac ctgagacagt gtgtaggggg cttctatgct ccagactaca tgaatgtggt
     1201 ttggcatgat gacaaatgcg taaatggcac agcgtttttc gcgtaccaaa aagcggaaag
     1261 agttgatgat gatgtctttc atggcatcgt caaacaggag ggatttccag ccgagaatag
     1321 aggcggtggt gaagtagagt tcagggtatt ggacgtgcat ttgtggttga attttgaggg
     1381 agtaaaaatg agaaaattat ccaaaatcat gatgattggc ttgtcaagcc ggcgcggacg
     1441 ccgggggaca gtttcggggt tggtgcggac accaaccccg gcgctgcnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttccaaatg cccgtcaatg gctttagctc
     1561 ttgcctgcat actgcccaag ccataccctt ccgatgcccc ggcgttttcg ggcaagccct
     1621 ggccgttatc tttgattgtc aaaataatag agccatcttg cagttgcagc gatacccgta
     1681 cctcggtagc gcgggcatgt ttcaccacat tatgcaaggc ctccttggcg atcaggtaaa
     1741 tgttgcgcct ccgctcgccg cccaacacca cattttcaag tccggcagtt ggaatgtcca
     1801 acacagcggc tatcttattg tccgacaata actctccagc taaggagcga aggcgcgagg
     1861 ccaaatcctg caaggtattt ttctccgagt ccatcgccca gatgatgacg cgcatgtttt
     1921 cgagggtttt ttcagccagt tgcgaaatac ggcgcagttc cggcgcatcg tcgtccaaca
     1981 gcaggtcttc gctcaggaat cggatggacg agaggctgcc gcccatatcg tcgtgcaatt
     2041 ctttagcaat gcgattgcgc tcggcgtgca gtgcttcctg gcgctccagc agggcgcgct
     2101 gccgacgcaa caccccccgg taatacaagc ctaccgagcc tccgaccaac aggatggcca
     2161 acagtacgct gccgattttg aaccaccagg tttgccaaaa cggaggctgt atccacacgg
     2221 ccattgaacg aagttccccc gtttcgccat tggcatttcg aggcagcact tcaagggtgt
     2281 attggcccgg cggtatcttg gtgaagcggg caaaaccgcc gttggccaca gttgtccagt
     2341 tgttgtcgta gcctcgcagg cggtatgcca gcgtactccc ttccgactgg tgatagccaa
     2401 tggccgtcag ttccagcgcg agggtatttt cacgataacc caagcgcagg gtatccgttt
     2461 cagctattac tttgggcgtt tcaaagagca ggttgttgac ccacaaggcc tctatgtgcg
     2521 cgcggggcct ggagcttgtc ggctgcactg cttcaggacg aaacgccagc ataccttttg
     2581 agtgtcccag aaaaatgcgc ccgtcgttgc tttgcaaagc tgcccaatat gcgaactgct
     2641 cgccggaaag gccgtcctgc tcaaaaaagc cgcgcagttc tctgctggcc ggatgatagc
     2701 ggtataatcc tcgcaaagtc cccaaccaca aataccccga tggagtgcga aagacactcg
     2761 ttacatctgc cccgttcagg tctgtgccac tgtctaatac tcgcttcaaa tgcccgtcag
     2821 tataaagcag caagcccgtg ttggaggcaa tccagatgga gtcgccacca ggcgtgatgc
     2881 aagctccctg tgtgtcgcca ccgatatcca ttcggttcac aatgcgcagc gattgacctt
     2941 ctaattttgc tacccaaagc tccttgctct caaagggaat cagtacggtc tgatttccca
     3001 caggaaatat ttcactaaaa aaatacccct tatgcgagga gaactccggg gcgggtatca
     3061 aggaaaattt tcccgcccgt tcctttacat caaaaatgcc gtcctgcgtt gtcagcaact
     3121 ttctcccggg aataccggta aaaacagcga cgaagcccag ttctacttcg aggcaggttt
     3181 cccagtcggc agcgcccttt ttcattcggt acaagccctg ctgagacagc ccccacaggc
     3241 cgccgttttc gtcgcaggac agttgtatga gcgggcgggc cagagcgggc ctctgttctg
     3301 tttttccgtc tttatacacc agcatcctcc cgtcgtttgt agccatgtac agcgcgccgt
     3361 cgggcatttg cacagcggcg cgaatgtgcg cgggattttc agacataagc tcctcaaatc
     3421 cgcgttgcac tatgcgggag taactcaggc cgccgctttc gtagcctacc catacttgcc
     3481 cttcccgact gatgagcagg cctctgggtg cagcattgag caaagaactt gtctgcccgg
     3541 cttgaggaat tacctgccct ttccattggc gccgcgccaa atcgaacaac agcagcccct
     3601 cgtttttagt acacgccagc aggctgtttt cggagggcaa ataagctccg ccgatnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnntgcgtt ccgcgcactg cgattgcgcc ataggcggaa cggatctgca
     3781 tttccgggga caaataattt tccgaaatcc aggtgtcgct gccggcagcg ctgtgtacgc
     3841 tcaccggact tttgagccag gcacaggcaa tgatgtgctg tggtacgcct ttgttgtcgg
     3901 cgcgcactgc aaaccgtacg ccttgcgtgg caaattgcgc cgcccagcgc ccgttctccg
     3961 gctggaaggt ccatacctga tcgcccgccc gaagccaaag ccggcccgtt tttttttcga
     4021 tatgaaaaac gtggtacgag cggtcggcgc catcgggagc gcggaagttg agcatacggc
     4081 cgtcggcgcg gcgcaggcag tgcaaagagt tgtaggtgct gaaccagagg ttgcccgcgc
     4141 cgtcttccca aaaactgctt tggataattt ggtcgggcgt ataggagtcc tcttcgttct
     4201 tgggtatcag gtagtgccgg agccggaggc cgtcgaagcg atagaaccca ttgagcgagc
     4261 caatccagat gaagccgcgg ctgtcttgcg agaggaagta attggccgtg ttgttgctca
     4321 gtccctgctc cgggccgagg tgttcaaact gccatgcggg tacctgagcg gtagcgcctg
     4381 ccaccgccaa caacatccaa ctcagtacaa aaacgacata aaccgagcag gtactccgtg
     4441 ccacgctgcc aaaaagcatg tgccgaaggt agggaaattg ttgagaaagc taagcgaatg
     4501 ggtgcaattt gctaaagcgc cgaatctgca tagaagtatt gtgctactga atactgctcg
     4561 ttcaacgaat ggcggctatt aggcatgaga agtttgaact gcattgttta ccacctgcgt
     4621 attgaaactc cgacgaatag ccgcccgtac agtcggcgtt aaaacaacgc cggagttgga
     4681 aaccttgctg taaacagcgc ccatcattgc cgtaaacaat tgccagtcag gcggacaagg
     4741 atgccccggc aacatggctg ctcctgcttc ttgtggtccg gtcgcatttt gagaacccca
     4801 gccagaggta ggttcgcttc cttctatttt gagcgagaaa taatcttctt catagatttt
     4861 gtctgcggta agtgcttgcg gtctgtgaga caacttcctg cccggactta ttttactgcc
     4921 ggaaatgatc aggcacttat aaagatcggt cattgcgctc aattggtcta aagggaaaaa
     4981 gacaaaatca atatcttcat agccatcaag ttgtttgtat aaattagttc cggcagcagc
     5041 aggcgccgag gttgttggag ataattcggc gtataatata gttttgtctg ctgaatcggg
     5101 cttctccaag atgtcgtaag ccgcactatc ttccaaagcg atagagcatg cacccggaaa
     5161 gaaatatgat gtcccatcgg aaggattgat gtcaaaacaa agctgcgtga tttccggagc
     5221 tttgacagtt cccttgttaa atttgacctg caaaccaatt agcggttggt ttgcaggggc
     5281 gttagacgcc ttttggggat caagcatttt cttccaatcg ctgttccccg tgtcaatttt
     5341 gtacgcaata cttcctgttt ttagtagcaa ggcaggattg ccgctgccgg gtgtggcatt
     5401 tgaagtagtc gtactgtcta ccctgttcaa attcaaagtt tgctttgcca tagtattctg
     5461 ttttttaggt tagatgttga aatacagtgc aatataacgc cggctcggca aaatctccaa
     5521 aaatcaagcg gagttttttg atggaatata accaacgtta tagatggtcc ggtttttaat
     5581 aaaaatgggc ggcgtggcgg gagttgcctt gtttgcggcc ggtctttgca ttataaggaa
     5641 gaggtcacaa taagtaaccg tgtggaaagt ctttcttcgc tgacgaaaaa agccgatgcc
     5701 ggagttccct ccgacatcgg ctttcgtttt tgttcatggg tgggtatatc aattgcttcc
     5761 tgcggcagta atccaggggc cgcacacgca tacacaggcg ccgccgggca gtgtgaaaca
     5821 tttgggcggg ttgagggtag tgcagtgaat ggtagagttg cagggcgagg cattgggcga
     5881 gatcgctttg aaagcgccgg cagcgttttg cgttccgaat tctactaatt ttccatttct
     5941 caccaccatc acgagcaggt cgccggtgcg ggtattgcgg cctacggtgt tgcggccttc
     6001 gcgtacgctg cactcgccgt ccacgccgtc aaatttgatg taagcggcgg cttgcggttc
     6061 cacgttcatt tggatgagga gaatgtgttc gcgggtttgc tgcgccgaag cagcaggtgt
     6121 tgcactgaag aacaaagcgg tgaaagctac ggctgcgatg gaaaaaaact tgaagatgtt
     6181 cattggttaa aagattaaaa aggtttgaaa aaaatggttt cggtgtgcag gctgccgagc
     6241 ggctgagcgc gtgttttgtg ttggtgcgcg gcgtgttcag cagcctgtac aaaccaatgt
     6301 gtcgggcggt ggaaagatgt acccagagga ggggaatttt ttttgaaaaa aagtgatcat
     6361 tatcccgtat cgcgagtagt taaaatctca aatcatgtac aacccagcaa agactttcat
     6421 ccagcggctt tctgacttcc aacacgattt tagcggcgac ccgcgctggg aactcgtttt
     6481 cagcaatgga aagcgcaagt ggttttacat agtcgtttcc tactacaacg gcatctacta
     6541 catctctgaa cgcatcggcg atcatgcccc catacaggtg aacgagcagg gcgaaatgac
     6601 ggccaacgcg cactatgggg gcgcccgcct tgatgaaaaa aagtggcaaa cgctgttctc
     6661 cctcgccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnattgg tcaggaaatt tccctacaag taccgccagg gcctgattca cagcaaatta
     6781 gtgcggcatt attgcccgga catctggcgc attgaccagg aactcggcga ggaaaacctg
     6841 cggaaatttg tgtctatggt cgaaaacaga cagatcaacc ctttccaaga cggcacgagt
     6901 aaaatcccct accttcgccc ctcaccaaca ccacttgcca tccatgaaaa catcatttct
     6961 attggcaacg gcgctcttgg cctgcatgta cgccggcgcg caatcgcggg gagacctcac
     7021 cctgatgagc ttcaacatac gtttcgacaa ccccggcgac ggcgacaacc gctgggacaa
     7081 acgccgcgat gatgtggccc aaaccatgct cttccatcag gcgcaggtgt gcgggctgca
     7141 ggaggccctg cacagtcaga ttcaggattt gttgcagcgg atgccgggtt atgcttttgt
     7201 gggcgtgggc cgcgacgacg gacagttgaa gggcgagttc tctcccattt tatacgatac
     7261 ccggcgcctg caattgctcg attccggtac cttctggctc agcgaaacac ctgaaaaagt
     7321 gtccaaaggc tgggatgccg cgctgccccg cgtggctacc tgggccaagc tgcgcgacaa
     7381 aaccgcccgg aaggtatttt ttgtcgtcaa tacgcatttc gatcacatcg ggcaggaggc
     7441 gcgcaaaaac agcgccgcgc tcatcgtgca gcgggtacaa caactcgccg gcagcagcag
     7501 ggctttcgtc atgggcgatt tcaatgcccg acccgatgag gcgccgatag ccgtgttgtt
     7561 gcaggcttat cgcgattgta aggcactgtc gcagatgccg catttcgggc cggaggctac
     7621 tttcaacggg ttcggcccca aagagcagga gggtatgcgc attgattata ttttttgcaa
     7681 cgaccccagc gtaaaagtac tgcagcacgc tactttgagt ggtacctggg gcgggcgttt
     7741 tgcctccgat catcatgcgg tgatggcaag agtgcggtag gcggtttctg tctgtgcgta
     7801 gtgaatggct ggcttcgccg aggtcgcacc ccgtagtcag cccttcacta aactacccca
     7861 ataactcagt gtggccgcat tttttgtagg gccagtctca atgcttccgg ctgctgtgtg
     7921 cccaacagga tgcgtttgtt gtttttatgg aggaccaatt gcagtcccat attgcccgac
     7981 acattgtaag ctgtgccgcc ggagccgtat ttgattcccc agccgccgta ctctttgagg
     8041 ggctggtatt ggcgcacttc ggcctcggct acttcctccc aggcgatgaa ccggctgccg
     8101 aaaaagggaa aaaaacggta ccgaatgccg gcacgggaaa gctctacctg taaactcata
     8161 aaataaaaag ccaccaacat gagcaacaaa aatacggcca cgccaatgag catttcgttg
     8221 ctgccgggat tgttgccaaa tggtatgcct tgcaccaact gaacgtaaag gccgtataat
     8281 atcagcccga aaggcatcag caacagtccc cacaaccacc actgccggaa gtattgccgc
     8341 tcggaaaaga ggatgatgtc ggtagccata gtattgattt tgtgctgttt gtattcaata
     8401 cattctaaaa accccaaaaa ccttactcct caccaccatc atcttgctgt tccaatgcag
     8461 gctcggatgc tccacaacct gccggttttt cgggcgagag gtagaggtct atctggcggc
     8521 ccgccgatag cgtgacgccg ggtgcgaaag ccggttcctg tttccagatg tagaactgat
     8581 cgctgcctgg cccgtcgccg tgtatggtgc cgatgttgag gttgaacgaa gtgatgatga
     8641 acaaagcctc ctcataagtt ttgcacacca gatcgggcac ggccaccatg ccggtaagtc
     8701 gctctgtaac aacaaattcg agcgtggcgc ccataggcac tttgaggccg ttgcgcacat
     8761 cgcgttcggt gtattgtttg ccctcgtaaa aaaagtgcag gatggtgttc tcctccaatt
     8821 tttcgtcaaa cttccgctcg cgcactttac tgcgcacatt cacccaggcc aatttccggg
     8881 agtactggtc gtaattgtag ctgcccacca tgtctggcaa tgccaccata tcgggcgtgg
     8941 ctttggtgac ggtgagataa atgcgccgac gctcttttac ccggctgagc ggggcgggcg
     9001 attgctccag caccatattg ggccgtgcgt ccactttgaa aatagaatca gtgaccacaa
     9061 tgcggaaatt gcggctgcgc gctttggcaa tggcctcgtt gagcgacatg cccacatagt
     9121 catgcacttg caccgactca ttgtggcggg tgtaaaaccg cagtaccaat tgcaataaaa
     9181 agatgccaag caccgcaaaa gccgccagag ccgccaggtt gcgcaagaaa ataccagatc
     9241 gaagaaattg tgccatttcg ctgagataat aagtcaatac tctgcccgaa ctgccctcaa
     9301 aatgaggctc ggtttgcttt ctcgtatcgc gccatgcgcc gggttcgccg tcgtaactca
     9361 cctgctgcgg ccgtactgcc ggatcgaaat aactttcctg agcctccggt tcatctgtta
     9421 tggcaggcat ggggagttcg gtcgtcgcag gggctgcagt tgcggaaacc ggctgtgcca
     9481 cggaaaccgg cgctttgcgg tcgaaggcaa aacgaagcac ctgatcgagc acgtcataag
     9541 gtttgaattg caagaaggct gcctgtcgga aaatacagga tgcgggcgta agggccggct
     9601 gcggatggat gtcggtcagg atggtttcgg cccggccatg ttcaaaaatg cgcacataag
     9661 cgctcagcaa agcatagcct tgtacgcctg tgatgcgggc ggcgcgctcc aaatcgcctt
     9721 ttacctgacc ggctattttg tggtaatctc cgcctgtact tccgcccaaa cgggccggag
     9781 tgatcagcgc agtattgggc gcctgtcctc ccggcacagc actgccgagc gccccttcag
     9841 aaagttccaa tacttcgtaa acaggcgttc cgtcgggctt gaaactcgtg aggagaccac
     9901 ctctgacttc gaggagttgc aaggcatttt gttgcgctac caaggactca aaaagtatct
     9961 gtggccggcg cggaatggat tccgtcattc tcaaccgcag cacccgacgg gcctcttcgc
    10021 tttccatccc ctgagggcga aatacgatgc gggtgtatgc ttccagatgc cggcgctcgc
    10081 tcaatgtttt gggcgcgtgg ctgccatcca aaggccgggc ggtgaaggga tagctcaaca
    10141 tgctctccac acggcggtaa aactcctctg cgctcgcttc gtactccgtt tggtgcgcca
    10201 caatctgatc gggaacactc aatccgttgc gcttcagcgt ttgcaggctt ctgtatttgt
    10261 cggcggcgat ggccgaagat acggcgtctg aaccagtgtg cggaagatcg gcttgttcta
    10321 cgcttttgag tagttttgac agcgtttcgg gggcgttttg aacgatgggc cacaccgcgt
    10381 cggcatgcag ggagagttct tcctgcgcca gcagtttcgg ctcagatacg agcgctccgg
    10441 cagcggtata ttttcgcaac aaaccggcgc attccgcacg gatggcctcc aacacgggcg
    10501 atgcctgcca atggtcgagc agggatctca aatcttcaaa gcgcttatgg aaaaggaatc
    10561 ctgtaggaat tttatacatc aaaagccggc cctggcggtc gtccaagagt acgggaaaag
    10621 gcgcatactt gccggatgcg gccagttttt caaaaacatt gcgcgcactt tccacgtctt
    10681 cgtcggcgcc actgaaagcg atggctatgt tgcgcttagt ggttgccgta tgccggagcg
    10741 catccagggc ggcgtccaaa ccttgcagca agtcggcata agcgctgcgt tcagggtgtt
    10801 ccgcccggcg ttcctgcaaa gaggcgcgca cgatgaaatt gaggagttga gtcggattca
    10861 gtccgatttc cgctgcctga tgaaaaagca gagaagaagg cagcagcggc gaggtattgt
    10921 tggcttcccg gatcagaatg cgcccgtcgc tgtaggcaaa accgtctatt cgggcgtaag
    10981 cgccgaatcc catttcctga aaaagcctga cgcactcgct tcggatatgg ctgatttgag
    11041 cttccggcaa acgggcaggc gtttcgcgct gggccatgcc gttcatgtag tctgaaaaat
    11101 aatcagacaa ttcggcgccc tgagccattt ctgtgggcgg cagggccgtt gccgnnnnnn
    11161 nnnnnnncga gccatcttca tttctgacga cgatgcagga aaactcgtgg ctgtcaatga
    11221 gttcttctac gatgacggcg ccttcgctca gggtgctttc aatcacaaaa accgcctccg
    11281 aacccggcag cgcttcgtgc gccaactggt ccaagtgcgc caacaggctt tcggggtgat
    11341 acaccaatgc ctgccctcct tcgtaagcca ccgttaccgg aaaacccagc ccgtcgcgca
    11401 ggtcggtgag cagccgaata taatcggttt tttcaaattt gcttctcgat ctccaggtgg
    11461 ctacgggcac tatctcgcgg aaaaaagcgc ggttaacggc atcgcggaag ccctcttcat
    11521 agccgggttc gtccaccacc gatacgccga tggatgaacc ctgtgtggcc gggcgcagca
    11581 ccaatgggaa gccgagggtg tcggcggctt ggcgataaaa gtcggcagcg gtgttgtctg
    11641 cgatccattg ttcgcgcggt atgacaaaag atttgggggt ataaaagcca tgagcaggca
    11701 gccactctct ttgcaaaact ttgtcggaac cgatttcggc agcccgaatg ccgctgccgg
    11761 agtaggggat gccgaggtcg tccaattggc gttgcaagcg cccgtctgcg ccgtattcgc
    11821 cgtgcattgc cagaaaagcg aagtcaataa gaccgggcaa ttcatccaag cgaatgcgac
    11881 ggcccacccg cgctacgagg gcgttgaggt cttcctgggc aagctcgccg aggctttcca
    11941 tatacacctg aaatccgttg ggagaatcgg gcaaatactc ggcaggcggg tatgcccgnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnntcgaaa acggcctggt ccaaactatc gtaaaccatc cgcccgccgg
    12121 cgaaggaaat ttcgcgttcg cgcgaagggc cgccgaaaaa gatacctgct cttagcttca
    12181 tggtgtgttt gtccgttgtg gttagatgag acaaaggtaa ttttttttgc cggggcgaga
    12241 atatgcaggg ccatgccttt ttactacctt tgttcaacta tggctgacga aaataccagc
    12301 gtacacaaaa aaaattcaac agctatgtca acctacgatc aactcattga aaaaggcaga
    12361 gccgaaggca gagaagaagg catcgaaaaa ggcatcgaaa aaggcaaaac cgaggtagta
    12421 ctgaatgcag ataccaacgg cctgtctata tcgcttatcg caaacatcac gggtctggaa
    12481 gaggctgcca tcctgcggat tttgcaggaa catggaaagg catgatgtga aaatcacata
    12541 gtttcggatt acttttgcgc cctcaatcaa aatccgaaca taccatggta cttccgcgga
    12601 tcaaccctgt tgaaaccaca gcctggcaaa aactgctctc ccattacgaa gccatcaagt
    12661 tggtgcaaat gcgtgatctg ttcgccgccg acgcacagcg gtttgataca ttttctctcc
    12721 ggtttgaaga cctgctggcc gattattcca aaaacatcct caccaccgaa acgctggacc
    12781 tgttgctgca actggcccgc gaaacgcaac tcccccaggc cattgcgcag atgtttgccg
    12841 gcgaaaccat caacgaaaca gaaaaccgcg ctgtgctgca caccgctttg cgcaatcgtt
    12901 ccggcaggcc ggtgctggtc aacggcgccg atgtgatgcc cgccgtccat gctgtgctga
    12961 cgagcatgga gcagttttgt gcccagatac acagcggcga ttggagaggc tactccggcg
    13021 agcgcatcac cgatattgta aacataggta taggcggctc cgacctcggc gcggtgatgg
    13081 tcaccgaagc gctgcggcct tattgggcag agggcattcg ggcgcatttt gtgtcgaacg
    13141 tggacggtac gcacattgcg gaaacgctgc gccgggtgcg gcccgaaagc acgctgttca
    13201 tcatctcgtc caaaaccttc accacccagg aaacgatggc caatgcctac agcgcacggc
    13261 agtggttttt ggaggccgcc cgcgatgagg cgcatgtggc caaacacttc gtcgctgttt
    13321 ctacgaatgc cggggccgtg caaaaatttg ggattgaccc cgcaaatatg ttcgagtttt
    13381 gggattgggt aggcgggcgc tattcgctca cttcggcgat tggtttgccc attgcctgca
    13441 ccattggttt cgataatttt cgtgaattgc tggaaggttt tcacgccatn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnttt tttcggtgca gaaacggagg ccattctgcc gtatgaccaa
    13621 tacctgcacc gctttccggc ttacttccag cagggcaata tggagagcaa tggcaaaaat
    13681 accgaccgca acgggcaatt ggtcggctac caaaccggcc ctatcctgtg gggtgagccg
    13741 ggcaccaacg gccagcacgc tttttaccaa ctcatccacc agggcaccaa gctcatcccc
    13801 tgcgatttca ttgcgccggc catcagccac aaacccatcg gcgagcatca taaattattg
    13861 atgtccaact tctttgctca aacagaggcg ctgatgaaag gcaaaaccgc cgaagaggtg
    13921 cgcgcagaat tggccgctgc cggcaagagc gagcaggaaa tagaggcatt gctgccgttt
    13981 aaggtgtttc cgggcaaccg gccctccaat tcgatcctgc tgcaacaaat cacgccgcgc
    14041 agtctgggca tcctcattgc gctgtacgag cataaaatct ttgcgcaggg catcatctgg
    14101 aacatcttca gcttcgatca gtggggggtg gaattgggca agcaattggc gaacaacatc
    14161 ctgccggaat tgcaaaacga cagcctggtg agcagtcacg atgcctcgac gaacggcctc
    14221 atcaatgcgt ggaaagagat gcgttcagcg cagggatagc cattgtatgt tgcagttggc
    14281 ccgcgccagg gataggaacg gaatggcggc ggcggataga gctgttagct ttggcgctgc
    14341 gatagccgga taaagctaac agctctgcgg agaggccgcc atgcagtgga tagcccggcc
    14401 ccgcggagtg taattgccgg tgcggggggc gccccaaaaa aatatttacc cttgtttggc
    14461 cgggtgttcc cgaaaaaaaa gaccgaagac cgccgcgatg acaaagccca gtactgccgg
    14521 cgtccaccag atggcttccc agtgtcgcac accgccgatg gtgaagtgtt tgaccaccca
    14581 accggccaga tgcgagccga tgagcgtgcc caagcccatg gtgaggaagg tgatgaaacc
    14641 ctgcacggtg cttttcagcg agggtggggt catgctgtct atgaatatct gagcggcgag
    14701 ggcggtgaaa atgaaacaaa ccccgtgcaa ggcaatagca ccgtacatga ggcctgtgcc
    14761 gccgagggcc agcatggcgt agcgcgcacc ccaggcaatg aagcccgaca ggaggacgta
    14821 acgcaacgga aaacgcaggg taaaccaggg cagaaacagc atgagcagca tttcggaaac
    14881 ctggcccagg gtcattttgg ccgccgcatg acctacgccg atgtcgttga gatagggatt
    14941 gacgaagctg taataaaatc cgccgggtat ggtgctgagg gccaagcaga tgaccaatac
    15001 gctgaaggag cgattgcgaa acagggcctt gatttcgggg ttgaaccact tttgcgcagt
    15061 cgctccgggc tgttgagcgc ccaagggcgg cgtgtgcggc aggctgaggc aatacaaact
    15121 ttgcacaaag gacatgagcg ccgagatgag catgggtgtg gccgactctt cgagggcgaa
    15181 ggcactgacc atgatgccgg ctacaatcca gccgacggaa ccccacacgc gcacgcgcgg
    15241 gtactctttg ccggggtcgg ataaatgtct gaaacacagg gccgcattga gcgaaaaggt
    15301 aggtgcgtac agcagggtgt aggggagaaa aaaggcataa aagctgccga aacttttcat
    15361 ttgagaagcg cccaagagca tgagaccacc caaaaagtgg aggatggaca gcaggcgctg
    15421 ggagggaatg tgccggtcgg ccagcacgcc catgaggaag ggcgtgagcg aggaggcaat
    15481 ggccgtagct ccgaaagccg tgcctaccag agcgccgtcg tcgcccagat gggccaagat
    15541 gtaagaaccc agcgtaacaa accaagcccc ccagaggaaa tactgcatga attgcagcgc
    15601 agaaagccgg aagtgcagga gcttggtcat ggcaggcagg gaactgcgct actcggcgcg
    15661 gaaaggatgc cgctgtaccc actgtacaga gggttcgagg aggcggatga agaaaggcat
    15721 gaggcgattg gatgggccgt gataatggcg caggcggcag tccaactgct gtggctcggc
    15781 gcctacggag cttttgattt ccatagcggt gcgggcgaag acgagttcgg aagcgcccga
    15841 ttcgatggcc gttttggcca tatcgtagag catattgagg tacagttggt gcgaggcatt
    15901 gagggcgttg cggaagccca agaaatgcgc ctccatgctg tggccgttgc gcatggtggt
    15961 gcaaaatcca accaactcgt cattttggta atagccgaaa acgcggaaat gctgcgggaa
    16021 agcctgcttg agggccgtga aatagccggg atgcagatcg aaggcgttga aatcggcgtg
    16081 ggaagccacc tcgcagtaca gctcgtacat ggcagcttct tccagcacga ccattttttc
    16141 ggtcagttcc cggcgggtga tgccgtccat ttttttgaaa gccctgcgcg cccgcacgcg
    16201 atatttggaa ctcatggctt cgaggtagtc gtcgaatcgc tgccaggaag gctggatgtg
    16261 gaggatcata ttgggctgga aactgaattc ggagaagcgc atccggctca gttgctcgga
    16321 aacgcctttg cagcaagggc tgatgtcttt gaccattgct aggtccactt tgcggccaca
    16381 gcaacgaaac cgctcggaga cggcctccat ggcggaatcg agcaggcggg cggtctgatc
    16441 ttcgcgcaat ccttccacag aaaaatgaaa cccatgcgcg cctgtgagga gcatgttgcc
    16501 gcaaaccagc aattggaaac gccccagccc ggccaatacc gattttattt gtttgaacca
    16561 gccgcgttct gcctgaaggc cggattcgag gctttttttg atgcgcaaat cgagcaattg
    16621 ggcataagcc aaaccgaccg ggcgcgcctg tttgtaaaac acgacataag caaaagccat
    16681 gccttccgga gcgcattgct ccatgacagt gaggtagttg cgttgcaaaa agatgtcgtc
    16741 ggagggcgcc gcgaggtccc agtcttcagg ggtttgttcc aatgatgaga atacccgcgc
    16801 cgtccatccg ggcaagggca acgccacttc attttccaag ctgcgggcag tagccgagcg
    16861 gcggcacaaa ccggcggttt cggcaacagg ggcatacaaa aaagagagcg cactttggat
    16921 ggcgcaaggt ttggaaacag cttcgggcgc agggtgcttc atggcatcat tggtaatggt
    16981 cggcaaagga aacgcaaggt gtgggggtat tgtttttgag tacctttgcc gctctgaaat
    17041 aacataccga atgagcgccc gacaaatatt cacgcccggc atccgacaca tgctggtggc
    17101 tgttttgagc ttttccacca tgaatttgnn nnnnnnnnnn nnnnnnnnnn nccgccatac
    17161 cgcctatgga attggtgttt tttcgctgct tagtgtcttt gctcatctgt ttgtacctgt
    17221 tgcacaaggc cggcgtccat tggctgggcg agaaccggct gatgctggcg ctgcggggca
    17281 tctgtggcac catggcgctg tttttgtttt tcattaccat caaaaaaata ccctttgccg
    17341 gggccgtcac attggcctac acctcgccgg tgttctcagc catgctgggc gtattgtttc
    17401 tgaaagaaaa gctgaagccg gttcaatggg tttacatttc catatcgctg ctcggcgtgg
    17461 cggtactcaa gggcttcgat ccgcgcatac cgatgctgta tttttttctg ggaataggat
    17521 cggctatgtt ctctgcgctg gcgtacatgc tggctcaaag ggcgggagca tccggtggtg
    17581 gtggtgctgc attttcagtt ggtgggcacg ctggccggag cggtcggcac ggcggttcag
    17641 tttcagatgc cgcagggttg ggactggatc atgctgctgg gtatgggttt gctcacacaa
    17701 ttggggcaat tgcaactcac caaagcgctg caaatcgagc agttgggcat tgccaccagc
    17761 ctcaattttc tgggcgtttt gtatgcggtt tttttcgggg tgttgttttt tcaggaacat
    17821 ctcacacccg gcaatatttt aggaatgatc ctcgtggcgg caggcgtggt tgccaatgta
    17881 gtgaccggca agcgggccga ggtcattgct accaccaatg tggtgaaacc gtataactga
    17941 gcgccggaaa cgctttgggc gcaatacttt ctctcgccgt cagcaaaaaa agcccgttct
    18001 ttgcgcctgc aatgaatacg ctcatcggca ccgatatttc tgctgcccgg cactttctcg
    18061 aagccgggca tcccatcggc attcctaccg aaacggtgta tggactggcg gccagcgccc
    18121 tttctccggc tgccgtggcg cgcatttttg aggtcaaaac ccgccccacc ttcgacccgc
    18181 tcatcgtgca tgtacccgat gccgcggctg cgcaaaacta cgcgctgcat tggcccgaaa
    18241 ccgcccgtcg tttggccgac gcattttggc ccggcccgct caccctgctg ctggaaaagc
    18301 gcgacatagt gcccgattta gtcaccgcgg ggcttcctcg cgtagggctg cgcgtacctt
    18361 cgcatccgct cgcgcttcaa ctgcttcaaa cgctcagctt tccattggcc gcgcccagcg
    18421 ccaacccatt cggctacatc agtcccacta cggcgcagca cgttttcgat cagttgcaat
    18481 cgctcattcc ctacattctc gacggcggcc cctgccgggt gggcgttgaa agcaccatca
    18541 tcggttttga ggatggaaaa acggtggtgt atcgtttggg aggccttagc atcgagcaat
    18601 tggaacaggt cgccgggccg cttacgctgc aactccatca gagcagcaac cccgccgcgc
    18661 cgggcatgct caccacgcat tatgcgccgc gcaaaccgct gtatctgggg ccgatagatg
    18721 aactgaaaga gcgcttcccg caattgcgcg ccggactgct cacattgtgt ccgttgccac
    18781 agaaatccgc agatatcctt tgtatttctc tgtcatcaga aggcgatctg aacgaggctg
    18841 cgcagcatct tttcgcagcc atgcgcctcc tcgatcagtc ggatgtggat gccattttcg
    18901 ccgagccgat gccggaaacg ggcctcggca gggctatcaa tgacaggttg aaacgggcgg
    18961 cggtgtagga tggattggga acaccaacat tcgtgtacac gcctttgaac tcacaaacac
    19021 aaaaataaac cgacatgaaa tgtccgaatt gtcaaacaag cattcccatt caaaacatca
    19081 acgtgcagac cgacattgga aaatgcgatg cctgtagcag tatttttaaa gtgtccgaca
    19141 tttttacgga tgtaaatccc cgattcaaca tcaaccatgc gcctgcggga gcctggtttc
    19201 atcaaaattt taatcagacg accatcggcg ctaccacgcg gtctccaatg gcgttctttt
    19261 tggtgccttt tatgctggtg tggtcgggcg gatcactggg cggcatatat ggctctcaga
    19321 ttgtctccgg cgaatttgat cttttcatgt ctttgttcgg catacctttc ttcattggct
    19381 ctgttgtttt ttggagcatc acattgatgg cgatctgggg aaaagtggag cttaccttca
    19441 acaggcaggg aggaaccatt tttaccggcg taggcaaaat cggcatcacc aaacgctttc
    19501 agtggaacga ggtagccaga attgaagaga gtataagcca atacaaatat ccgggagggc
    19561 atggcgctca gatcgttttt gagggtaaaa acagacatac ctttgggtcg ggactgaacg
    19621 aacagaggcg ctactacctg ctgcaagccc tgaaaaccat gcatgcgcaa ggacggtaag
    19681 gataaaaatt cctcaaaatt cctttctttt gcagccatga acaacagctt tctcaccgtc
    19741 gtttcggtac tgcatacgcc aggcgatatg cgcgtgctgc ccgaattcat gcgcggcctg
    19801 tggcctgtgt tgcaagggca ttttgctgat tttgagatca ttctggtcaa taactgcacc
    19861 gaactcagcc ccgatgaata cctccgccca ttggacccgg agttgaaaca aaacatctat
    19921 gtgctgcaat tgtcggcccg cgtgaaccgc aaccacgccg tagtagccgg cttggaccgg
    19981 gccaacggcg actatgtggt cgtttttgag cctgggtttt atcacaaacc ggaactgatc
    20041 ctcgacttgt ttgccaaaac ccgcgagcat ttcgatatcg tatatctgcg gggtcgcagc
    20101 cggcctatca gtctgaaatt caggccgttt tacaaggcnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatca tctcgcggcg
    20221 ggcgctcaac tcgctgctgc gcctccgcga aaacctccgc tacatgaagg ccatctactc
    20281 cattgtgggc tacaacaccg ctgctttgga taccgaccaa aaaccggaat gggacgaaga
    20341 agacagtttc aacgagcgtt tcggcacctc cttggtggcc atcacttctt acaccacctt
    20401 cctgcgctcg ttgctgctgt ggatttttat gttttcgctc acttttctca ttgccgtgat
    20461 tgtcaatgct ttgaaggtga aatttaccgg cgtggacctg ctgggcaatg taggcgaacc
    20521 cttctccggc tggacttttt tnnnnnnnnn nnnnnnncgc ggctacctgc ctcaatttgt
    20581 acatcatgtc catctatctg tccaatattt acaacgaaat caagcaaagg ccgctgtaca
    20641 tcatcgagtc tattaaaagg ttttagcggt gggcagtagg cggtgctaag cgcgaagatt
    20701 cagtaggcgg taggcggtgc taagcgcgag gtgtcccggt aggcggtttg cggtgctaag
    20761 cgcgagggca gcgcggcttg cagcttgcag ccagcagcca gcagccagca gccattggga
    20821 aatgctaaat aaacacgaaa caattatatt tgcccttcaa cactcaacga caacatgaaa
    20881 aaccaacgca cgctctccct cctgctcttc ttttgcatcg ccgttgccac ctccttgccg
    20941 gctcagaaga aaacccaaac acctgtaacg cccacgcctg cgccagctgc tgccgaattc
    21001 aacgcgggct tgtacaaggg cctcaaatgg cgcaacatcg gccccttccg cggcggtcgt
    21061 tcggtagccg tcagcggtgt gccgggcgat gaaaatacct actacgcagg ctacaccggc
    21121 ggcggactct ggaaaaccga cgatgccggc ctcacctggc ggcccattgc cgacggcgcg
    21181 ctccaaaccg gctccgtagg cgatattgcc gtagcgccct ccgaccccaa tgtggtgtat
    21241 gtgggcatgg gcgagcacgc accccgcggc gtgatgactt cttacggcga cggcatctac
    21301 aaaagcaccg atgcgggcct gagctggaag caaatcggtt tggaaaaaac gcgccatatt
    21361 tccgacgtgg tagtacatcc cggcaacccc gacgtgctgt gggtggccgc tcagggcgcc
    21421 ctgtatggcc cctccgaaga tcgcggcgtt tataaaagca ccgacggcgg ccaaagctgg
    21481 aaaaaagtac tgtatgtgga tgaaaattcc ggcgcttcca gcctgagcgt ggacgccaac
    21541 aacccgcgca tcctctacgc cgccacctgg caacaccgcc gcctgccctg gaaagtagaa
    21601 agcggcggcg cgggctgctc catctggaaa agcaccgacg gcggcgatac ctgggccaaa
    21661 atcaatgagg gattgccaaa agaaatgggc aagatcggcg tgtcggtctc gccggccaat
    21721 tccaatcgtg tatatgccat tgtggaagcc gagaagtcga aagccggcct gtatcgctcc
    21781 gacaacggcg gcaagagctg gcaactgatg tccaacgacc aaaacatcac ctcgcgctcc
    21841 tggtattaca tggaggtctt cgccgacccg caaaacgccg acatcgtgta tgtgctcaac
    21901 gcgccgatga tgcgctccat agacggcggc aaaaccttca gcaatatctc cgtcgggcat
    21961 ggcgacaccc acgacctgtg gatcaatccc aaaaacaacc aaaacatgat cctcggcgac
    22021 gacggcggcg cagagatcag cttcaacgcc gggcgctcct ggtcttcgct gaacaaccag
    22081 cccaccgccc aattttaccg tgtgaatgca gataagcagt ttccgtacaa aatttacacc
    22141 ggccagcaag acaacacctc tgtcatcatt gcttcgcgca acaacagttt cggcatcacc
    22201 gacaaagact ggaccaccgg ccccggctgc gagtgcgcct gggttgcctt tgatcccgac
    22261 aagccggaaa cactctatgg cggctgctat cagggctata ttgacgccct cgacacccgc
    22321 accggagagt cgaaagacat catggcctat cctacgctca atctggccat acagcccaaa
    22381 gacatgaagt accgcttcaa ctggaacgcg cccatcgtcg catcgccgca caacgccaac
    22441 gtgatttacc attgcgccaa cgtggtgttg cgcagtgccg acggcggtct gcgctgggaa
    22501 gtcatcagcc ccgacctcac ccgcaacgag gcggccaaac aaggcgacgg cggcggtccg
    22561 attaccaacg aaggcgcggg cggcgagaat tacaacacca tttattactt cattgaatcg
    22621 cccatcgaag cgggcgtact ctacaccggc agcgattgcg gcttggtgca tgtcagccgc
    22681 gacaacggca aaagctggct caatgtgacg cccgccggac tgcccgaatg cagcattcat
    22741 tccatcgaag tatcgccgca cgacaagggc acggtgtata ttgccggaac gcgctacaag
    22801 ttcaacgact ttgcatctta cacctataaa tcaaccgatt acggcaaaac ctggacccgt
    22861 atttccgagg gcatccgcac cgatgatttc ctcaaagtca ttcgcgaaga ccccaaaacg
    22921 aagggattgc tgtacggcgg cgccgagcgc ggattttacg tttccttcaa cggcggtcag
    22981 cgctggaacc aactccaact caacctgccc gttgtacccg tcaccgacct catcgtgcgc
    23041 gacaacgacc tcgtggcctc caccgccggc cgcgccctct ggattttgga cgacctctcg
    23101 ccgctacaac aatcggccgg taagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nagttcattc ccggcatcgg caagaacccc gacaacggcg tcattctcag ctacttcctg
    23281 ccggaaaatg ccgacagcaa cgaggtgaaa ttagaaattt tggatgctgc gggcaaagtc
    23341 attcgcagtt tcagcagcaa aaaagacgag gcgttcaaac cctaccccgg cggaccgcgc
    23401 cccagtccca ccctgcctgc catgaagggc ctgcatcgtt ttgcctggga tttccgcacc
    23461 ggcggtctgc ccgatattcc cggcgtgtat gtgtatgccg actacaccgg ccatcgcgtg
    23521 gcgcccggca agtacagcgc ccgcatgtcg tacaaaggcc agacttctac ggcggacttt
    23581 gaaatcgcac ccgatccgag gctgcaaacc gcgccgcaag actgggccga acagcagcaa
    23641 accatcgcgc gcgtagaggg gcacatcagc gatatgcacg agtgtgtcaa tgctttgcgc
    23701 aaggtgaaaa agcaggtgga acacttcaat gaatcgttca aaaatgctgc gggcgacaac
    23761 gaaaaagcca tgctggccaa cggcgagcaa ttgctgaaaa aaatggctga gtgggaagcc
    23821 aacatcgtgg aaacccggca gcgcaatttc caggatgtca tcaactggcc cagcaaactc
    23881 aacgctgagt tctttgatct gcgcggcaaa ttggacgtac acgatccccg tgtcacgcag
    23941 ggcgtccgcg accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 ncacaaagca acctggaaaa agctgataga ggaggatgtg gcagggtaca accgcttgtt
    24061 ccgggagagg aatttgccgg cgctttttgc acctcagagg taaaaacatg cgcttctctc
    24121 tcttccaact cgccgatgcc atgcagggcc gcgtcatagg gcaggcgcct gccggagccg
    24181 tggtagaaca cctcatcacc gacagccgcc acatagcctt tgcgcccacg gcactgttct
    24241 ttgctctgcg cggaccgggt cgcgacggac acgctttcat cgccgatgcg taccggcaag
    24301 ggctgcgcta ctttgtcgtg gcgcgtttgc cggaagtggg aagtctgccc gaagcctgct
    24361 tcattcaggt ggccgacacg ctgcaagccc tgcaaatgct ggccgcctgg cgacggtctt
    24421 tggcagctat acctgtgatc ggcattaccg gcagcaacgg caaaaccatt gtcaaagagt
    24481 ggctttttca attgctgcac cccgaccggc atgtcctgcg cagtccgcgc agctacaatt
    24541 cccaaaccgg cgtgccgctc tcggtgtggc aactgaaacc cgaacacgag ctggccctgc
    24601 tcgaagcagg catttcccgc atgggcgaaa tggctaagct ggcgcccatc atccaaccca
    24661 cgttgggctt gttcaccacc ctcggcgatg cgcacagcga aggcttcccc gatgcggaaa
    24721 ccaagctcag ggaaaagctg atgctctttc agtcggcgca gctcattttt tacaacgccg
    24781 acgatgcccg tgtggacgcc gccatacgag cgctcggcaa gcccttattt tgctggggaa
    24841 gcgccgccca tgccgacctg caactccttc gagcaagcag ctccgacgac cgccgcagca
    24901 cccgcatcga ggtccgcttc gaggggcgcg aacggagcat tgacatccct ttcagcgatc
    24961 aggcctccat ccacaacgcc atgctgtgct ggtgcgtact gctccatctc ggcttgccgg
    25021 acgaactcat ccgcgaacgc atgagccgcc tcgaaccggt ggaaatgcgc ctcgaaatgc
    25081 gcgaggccgt caacgcctgc acgctcatca acgacagcta caattccgac ctcaacgccc
    25141 tctccatcgc cctcgatttc atggcaagac aggcttcggg ccgccgccgc acgctcgttt
    25201 tatcagacat tctggaaagc agccgcgccc ccgaaacgct ctacgcgcag gtggccgatc
    25261 tgatttgtga aaaaggtgtg gagcgcctca tcggcatcgg cgggcaggtg cagcgcttgc
    25321 aaggcaaact ccccgacggc attgccaccg ttttttacaa cgatgccgac gcgtttttgc
    25381 agcagcacga catggagact ttcagtcggg aaatcatcct gctgaaaggt gcgcgccgct
    25441 ttggttttga acgcattgca gacaggttgg ccctgaaagt acaccaaacc gtcctcgaaa
    25501 ttcacctcgc cgcactcact cataatctcc gccagtttca gcaactcctc gcgcccggcg
    25561 tccgcaccat cgccatggtg aaagcggcgg cctacggcag cggcagcgtg gaagtggccc
    25621 aactgctgga acttcagcgc gtggactacc tcgccgtagc ttatgccgac gaaggggtgg
    25681 aattgcgccg gggcggcatc ggactgccca ttttggtact caatcccgaa gaggccgttt
    25741 ttgacatgat gctccgctac cgcctcgaac cggagttgta cagcctgcgc ctgctccgca
    25801 attttgcgcg cttttgcgcc aatcggcaag agccggtacc cgttcacctc aaggtggaaa
    25861 ccggcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgaaagcgc ctggcagcgc
    26041 atcagcgcgg gtttgggcta ccgtccgctg cgacacatac tcaacaccaa cggcattgcc
    26101 cgctttccgc aataccagat ggacatggtg cggctcggca tcggcttgta cggcattccc
    26161 accgaaaacc ttgatttaca gcctgtactc tgcctcaaag ccgccatttc ccaggtcaga
    26221 accctgcgcg agggcgatac ggtgggctat ggtcgcagag gcgctgggca ggcaggtatg
    26281 cgcattgcca ccatcagcat aggctacgca gacggcttgt tgcgcgctg
//
LOCUS       DAORLF010000011        25374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1061_length_25374_cov_2.079150, whole genome shotgun sequence.
ACCESSION   DAORLF010000011 DAORLF010000000
VERSION     DAORLF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_25374_cov_2.079150"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..983)
                     /locus_tag="PK198_01995"
     CDS             complement(99..983)
                     /locus_tag="PK198_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRF37532.1"
                     /translation="MRTYPAKVLLFGEHILLRGAAALAAPLHLYGGQWQFGSDSGQQF
                     RLREWVEYLLQLPCDFRLAAFKDDLEAGLFFQSDIPTGYGLGSSGALCAAAYERYANQ
                     PIARHDASLLPELRNILAAMESFFHGASSGIDPLVSYLNQPLLLRPDGALDAVQVPAL
                     PPGMTLFLLDSRRTRSTGPMVQYFRQRCEDPAFETLMRTELLDVSDAAVSAWLDHRME
                     DFSRHFQCISRFQAQHLAPMIPDEVLQIWQEGNARGAFSLKLCGAGGGGVFLGLAFTP
                     LFEHEGWIGDFPCHVLAV"
     gene            complement(1048..2736)
                     /locus_tag="PK198_02000"
     CDS             complement(1048..2736)
                     /locus_tag="PK198_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF37533.1"
                     /translation="MKFNTGFDLKHLRGDLFGGITAGIVALPLALAFGAQTELGAIAG
                     LYGAIALGFFAALFGGTPVQISGPTAPMTVVSAVIIAESTQRAGSLQEAIPLVLAVFF
                     VAGLLQVLMGVFRIGKYIRYIPYPVVSGFMSGIGVIIIIGQIFPLFGAMTPTGGSLAV
                     LKSLDDLPRVIDWEAVVLSIIAVVLVYFSPRIIKAVPSTLIALLGVSLLGYLVFRGEV
                     GLISDSSGGKMPSGVPTLHLHFFAPLLVPANWKLILEYAATLAALGAIDTLLTSVVAD
                     NITKTKHDSNQELVGQGIGNMAAAFIAGLPGAGATMRTVINANSGGKTRLSGIVAALL
                     LLAILLGMGPLVGQVPTAVLAGILLTVGIGIIDYKGIRNIRAIPRSDAIIMMAVLVLT
                     VFVGLLEAVAIGMLLASILFMKAISDVVEHRTVSAPLTSYSSEIPWNDEIGLLEKVGS
                     KVYIKHLDGPLFFGFASRFQEMSKVMPGVKVVVFRMKKVPYIDQSGMLAMEEAVLDLQ
                     RQGITVAMTGLQQQPKAMLEGIKLIPNILPNHLVFKNFDACADWLREFCTAETA"
     gene            complement(3118..3720)
                     /locus_tag="PK198_02005"
     CDS             complement(3118..3720)
                     /locus_tag="PK198_02005"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRF37534.1"
                     /translation="MIKMLLSLLVLLCFGAAQGQDYSCRIKTSEGRTLYIHTENKFEI
                     DESGFGREGIHVETNNGEIQRFSKGKYMIKPNSMGDCVISIYNKEKSKKIHSELFFVV
                     YSVQPVIIPVFYSQEYQMTDNEIPIDRLEDLRGFSTMVYSRNQIIFLVEEMKLIIRSK
                     KKGEFEFVTNGNLSKEIKAKINSLDKGDLILLKNIDKIRY"
     gene            complement(3851..>5067)
                     /locus_tag="PK198_02010"
     CDS             complement(3851..>5067)
                     /locus_tag="PK198_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF37535.1"
                     /translation="TDETQEVVRRKVEEYKAKGLQIEQVRRKERKGYKAGALKDGMEV
                     ARGEFMAIFDADFLPRPDFLKNTIPHFRDPEIGVVQTRWEHLNEDYSLITQLQALQLN
                     VHFTVEQRGRMAGGMLLQFNGTAGVWRRKAIEDAGGWEADTLTEDFDLSIRAQLKGWK
                     IKFLEEIGSPAELPVEMNALKSQQFRWMKGGAETAKKMLPVVWHSPLSRWKKFHATMH
                     LLGSTIFLFVLTASIASVPLLYFLGELIAGGFNKNSFAYFMIGMLSIISIYYVANLQS
                     EAQRKNEPFSKSVYRFILHFPLFLSLSMGMALHNSRAVLQGYLGKKSAFVRTPKFNIL
                     DKKQDSVKGKYMMRKLEWTTIGEGLLALYFLGAVALAFYVQNTTFLVFHLMLAFGYST
                     IFFYTLRHRSLG"
     assembly_gap    5068..5077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5078..>5386)
                     /locus_tag="PK198_02015"
     CDS             complement(<5078..>5386)
                     /locus_tag="PK198_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF37536.1"
                     /translation="VLQFDLLYHYKKSKKQAAGASPGQDNAVKQKAVAQLALAGDDSG
                     ATAFPSAADMSMSEDERNKEYPFVTVQLPLYNEMYVTERLIDCIAQFDYPTDRFEIHV
                     L"
     assembly_gap    5387..5473
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5755..6921)
                     /locus_tag="PK198_02020"
     CDS             complement(5755..6921)
                     /locus_tag="PK198_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF37537.1"
                     /translation="MDIYTQKSRWKLYLGIAGALIVLISLVYTTMLTNRLALEERNKV
                     NIILKAQEEMAKEPGEDCIPCLSCMETNFYLDVIQSNKTIPMILVGSDGMIQDAVNMG
                     TDDPASWEKQLKKMQESDLKPIQGLDFALYYSESNILKQLRFFPLIQLLLIAAFVAMG
                     YVGFSNARRAEQNRVWVGMAKETAHQLGTPISAIMAWIEHLQLVLEEHTDTEDVLLEL
                     NKDVNRLQLVADRFSKIGSAPVLDTVNIYDELEECRSYMQKRASRRVNFHFPDPASSP
                     ALSVPLNPPLFDWVIENLLRNALDAMDGQGSISAEVTEDDRYVSILITDTGKGIPTSK
                     FKTVFEPGFTTKKRGWGLGLSLTKRIIEDYHKGRIFVKWSEEGKGTTFCIQLPK"
     gene            complement(7074..8147)
                     /locus_tag="PK198_02025"
     CDS             complement(7074..8147)
                     /locus_tag="PK198_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRF37538.1"
                     /translation="MSHTVIIGTGAYLPTEVVTNAAFETNEFYNPDGTPIDRPTKEIV
                     DKLESITGIRERRYAKPELRSSDIAAIAARRAVDDAGIDPETIDQIIVAHNFGDILSG
                     TLQTDILPSVASRVKYSLGIKNVHCVPYDVIFGCPGWVQGLIQADAFFKAGIAKRALV
                     IGSETLSRIVDPHDRDAMIFSDGAGAAVLEYQPTEGKKGIIASAVISHAQEDTYYLFM
                     GPTNKPESPDNHLYIKMHGRKISEYALSHVPTAMKECLDRAGVQHISEVSKIFIHQAN
                     EKMDDAIVRRLFRLYNVREYDAQVAPMNIHDFGNSSVATIPTMYDMVRRGQLGEHRVQ
                     EGDLVLFASVGAGMNINAVAYRY"
     gene            complement(8587..>8984)
                     /locus_tag="PK198_02030"
     CDS             complement(8587..>8984)
                     /locus_tag="PK198_02030"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRF37539.1"
                     /translation="KRLAETSRTALIDIVTIILGLTVGASTQADVFLTPQSVMIFALG
                     AFAFGVSTAGGVLFAKVMNLFLKPEDRINPLIGAAGVSAVPDSARVAQLEGLREDPSN
                     HLLMHAMAPNVAGVIGSAVVAGLLLSFLG"
     assembly_gap    8985..8994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8995..9899)
                     /locus_tag="PK198_02035"
     CDS             complement(<8995..9899)
                     /locus_tag="PK198_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRF37540.1"
                     /translation="MKKAGVIFFVIALLATLHGLIARSDQQQADVQSETTHQGSEAVG
                     TFFHYTGFANFTAGHGIMIAVGLLFIFLAIKYEYEPLLLVPIGTGIIIGNIPFQAGAG
                     LQIGIYEPGSVLNYLYYGVVKGIYPPLIFLGIGAMTDFSSLISNPRLMLLGAAAQLGI
                     FTTFLGALILGFHPAEAGAIGIIGGADGPTAIFLASRLANGVREIGQTVGGDPVYARN
                     LIGPIAIAAYSYMALVPVIQPPIMRLLTTRRERLMRMKPPRAVSRTEKMLFPIVGLIL
                     TTFISPSALPLLGMLFFGNLLKESGV"
     gene            complement(9896..>10141)
                     /locus_tag="PK198_02040"
     CDS             complement(9896..>10141)
                     /locus_tag="PK198_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRF37541.1"
                     /translation="TISRSTSTDTVPVKSPLPGTVTAVLVAPGAVVNKGDTLLKIEAM
                     KMENNIHAEKPGKVVSVRIKPGDAVLQNDVLIEMEAS"
     assembly_gap    10142..10245
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10339..10674)
                     /locus_tag="PK198_02045"
     CDS             complement(10339..10674)
                     /locus_tag="PK198_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37542.1"
                     /translation="MEQLSYGILISIVGYSVTLLTLALLYYAFRYLPKLLHLQIRRRL
                     QKLGHHVAHNEDLSVKGDEAAAIAMALHLYFNELHDEESTRITIRKIPKTYSPWSSKI
                     FGMRHPIRR"
     gene            complement(10869..12428)
                     /locus_tag="PK198_02050"
     CDS             complement(10869..12428)
                     /locus_tag="PK198_02050"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRF37543.1"
                     /translation="MDRHDKIRQLVELREQARLGGGEKRIAAQHSKGKYTARERIEKL
                     LDEGSFEEYDMFVTHRSTNFGLEKETYLGDGVITGRGTIDGRVVYVFSQDFTVFGGSL
                     SETFALKICKIMDQALKVGAPVIGINDSGGARIQEGVNSLAGYAEIFYRNIMASGVVP
                     QISAIFGPCAGGAVYSPALTDIIIMSDKTSHMFVTGPKVTKTVTGEDISTEDLGGADI
                     HTGKSGVAHFKAESEDEGLLLIRKILEYLPQNNLEDPIATECTDPVDRMDDQLNEIIP
                     DDPNQPYDVKDIIHIIADNSEFLEIQRHWAKNIVIGFAKFGGMPCGIVANQPNFLAGV
                     LDINSARKAGRFVRFCDAFNVPILTLVDVPGFLPGSFQEYGGIITHGAKLLFAYGEAT
                     VPKITITLRKSYGGAHDVMGSKQLRADINYAWPGAEIAVMGAHGAIEVLESKALSELK
                     DPEERAAFFNAKEEEYREKFANPFKAASFGYIDDIIEPRNTRPRIIRALRALASKKDV
                     LPPKKHANIPL"
     gene            complement(12528..13256)
                     /locus_tag="PK198_02055"
     CDS             complement(12528..13256)
                     /locus_tag="PK198_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37544.1"
                     /translation="MNTNIAFLELKYGNIYGGYPNFYAISEIALLVFETGSNRIFLES
                     WVNNANVEIVNVYPKVNELGHTIGRAKEVVNLKTRRRKPYKDDFRMEEMELKYAFKQL
                     HPAKMAIRNFLMKNLKKYQFRDIITFDGRRDVFLCERSGVNFSRYNILDLQKVLNKET
                     EYLFSLNKLSVVINFELDNSYLRSNNLEYWLHPIAARQIIPKSAAFDAARLMMVYNEY
                     TQFHEDFMRRGALLLNKIQNLKEE"
     gene            complement(13399..13566)
                     /locus_tag="PK198_02060"
     CDS             complement(13399..13566)
                     /locus_tag="PK198_02060"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRF37545.1"
                     /translation="MALGQSYLQTGGKNQSAVHWDMIADMKNGGMIYADDEKIYENGN
                     FLFLDTNSLLG"
     assembly_gap    13574..13805
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13806..14530)
                     /locus_tag="PK198_02065"
     CDS             complement(<13806..14530)
                     /locus_tag="PK198_02065"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRF37546.1"
                     /translation="MKNILTKYAELLVHYCMEVKPGDRLYVNSTMLAEPLIREVYRTA
                     LQAGAAVDVDMDFREKNRILINEGSAFAQQHVSPAYRQAMSEYEAYLFIKAPFNLRED
                     NGLNPDNGALRREALKPMQQEYFRRSATRALKRNFCLFPTDAGAQEAGMSLEEYQHFV
                     YNACRLYDDNPMESWLSVRRNQQQIVDLLNSRENIRYVGEGIDIRFSTQGRVWINSDG
                     QTNMPSGEVYTSPVEDSVNGVIH"
     assembly_gap    14576..14593
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14769..15986)
                     /gene="hflX"
                     /locus_tag="PK198_02070"
     CDS             complement(14769..15986)
                     /gene="hflX"
                     /locus_tag="PK198_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRF37547.1"
                     /translation="MAKEWTLGEKDNKSIAKETEYAVLVGVICQNETEALVNEHLDEL
                     EFLALTAGAECIQRFTQKMTHPDPRTFIGKGKLDEIREYIERHEQVNMVILDDDLSGK
                     QVNILEEALKVKIIDRSSLILDIFARRAQTAQAKTQVELAQLQYMLPRLRGLWTHLER
                     QRGGIGLRGPGEKEIETDRRIVKDKISQLKEKLQVIDRQSATQRKTRGELIRVALVGY
                     TNVGKSTLMNLLSKSEVFAENKLFATLDTTVRKVVFEDMPFLLSDTVGFIRKLPHHLI
                     ESFKSTLDEVRESDILIHVVDIAHPQHEDHIRTVEETLRSLDALDKPTILVFNKIDLY
                     RTLNYDPFVDDEARKEIEGGLRGNLRYMFGKDNVFMSAATKEGLAELREKMTRLIKQQ
                     YVVRYPYQAARWK"
     gene            complement(16119..17246)
                     /locus_tag="PK198_02075"
     CDS             complement(16119..17246)
                     /locus_tag="PK198_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF37548.1"
                     /translation="MKLVQEINQTNQKLQETQKNKAATLDRYIALRSQVKKRQQLVAT
                     MQEEITHADSAIARSELVMGALSEDIARLRNEYAQMLRTAWRLRLQQSHLAFILSSKS
                     LNQALKRRQYVRQYERYRRRQAKLIEETQEMLSNKIAQLEKRKQEQRNLIVSVEEQTE
                     LLRGEMVNKDKALQSLKADEGRLVKDLERREKERERFSAAIEQIIRNEMAQKRKEART
                     APPPRKITSPAPASRDDSPEPAAAIADNSGFGARKGSLAWPVGGGRISRTFGPQPHPT
                     LKGVTVPNNGVDISASAGSAVSAVHAGKVVGVQFIPGYQNMVIVQHGAYYTVYSNLES
                     LSVGKGDEVGAGQAIGKLGNEDLHFEVWREKQRLNPAGWLR"
     gene            complement(17344..18126)
                     /locus_tag="PK198_02080"
     CDS             complement(17344..18126)
                     /locus_tag="PK198_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRF37549.1"
                     /translation="MRKANFHSILLIFSAIILFASCGPGRKVGKTDLKANTPEAMLKL
                     MAARKIDVQWMEAKAKLDLNDGSQSIKANAVIRMKKDEWVWISVKKFGFEGGRALIRK
                     DSAFIINRLNNEYYAEPLSYLEKTYGVPADLVALQNLLLGNPVFLQTSGFQLDESPEH
                     YRLSGQGGGFENRFQINKTDLTLAQMELEDPKKLQSVRYSLSNYGELAGNRKFSYLRL
                     LELNSPSSGKMSAGIEFSDVEINVPKDNKFEIPEKYTRVQGK"
     gene            complement(18278..19945)
                     /locus_tag="PK198_02085"
     CDS             complement(18278..19945)
                     /locus_tag="PK198_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37550.1"
                     /translation="MHAGSAWGQQITQAAAELEKMFVEACRERAIGNYEKAVPLLEGL
                     LKKDNKNHAAMYELARVYDAQNKYAEAEKLMVSATALSPENNWYKKFLADVYQKTNKF
                     LSAATLYEQLVKNDAHNDAYYYRWAYFLVKADAIDRAIKVYDELEKNNGISEESVRRK
                     HSLYLGLGNNKKAAEELQRLIAAYPKRTEYRHLLAGFYEQIGDTAKAQAEYRQILSIA
                     PNDSRAQLALAGNGRNAANDDIAYLRALKPVFENPAANIDQKIGKIIPFIQKVADTRN
                     TELADVVLELTSILEKAHPGEAKPLAAAGDLFYYTGRKKEALAKYLRALDLDKGVFTV
                     WEQAMTIYAENGQYTALLRMSENALDYFPNRAMAHYFNAWALLETGRSSDAADVVEQA
                     MLMIGKDQRAAALLLGVKAGIAHVQKKHPAADVLFDEAVKQYPASGELLAQYSLSLLA
                     RNEYTDKALEMAQQADKLSPGNVAVDHAMGWALLKKKDFAGAKSRLSKALKTNEEELP
                     LLLEHYGDLLYQTGDIDGAVNYWAKAQAKGGRSSLLDKKIADRRWYE"
     gene            complement(20008..21009)
                     /locus_tag="PK198_02090"
     CDS             complement(20008..21009)
                     /locus_tag="PK198_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF37551.1"
                     /translation="MNLIIPMAGRGSRLRPHTLTIPKPLLPIAGKPMVQRLVEDIAAS
                     LPEKIEEIAFVVGDFGQEVENRLCDIARQLGAVGRIYRQNAPLGVGHAIYCARESMTG
                     RCMIAFSDTLFRATFAFDPDEDGIIWVKSVPDPVSFGVVKVSEDNVITDFVEKSPVFI
                     SDLAIVGIYYIRNGQQLRDELAYMLDNDIKDKGEFQLTTALEGLKQKGLRFRPANIEE
                     WLDCGNKDNVLATNRRMLELKHQEEELIDESAEVIDSVIIPPCSIGAGAVVRNSVIGP
                     FVSVGSNSGLHRSVISSSIVQSETVIHNANLINSLIGNNVDYSGEASEISLGDYSKM"
     gene            21177..>21999
                     /locus_tag="PK198_02095"
     CDS             21177..>21999
                     /locus_tag="PK198_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37552.1"
                     /translation="MNRFVFFFLIFTASCSQDQATKNQMADLDSAYQEVLILAEKTNQ
                     QAEATCRMKENFIHALQPLPYLMADIKAVGNLHTALQTQLNATLNAAENLPADSPDWA
                     VPPATISSIQSSFKAFEEAVRAHFPDEEALPDTPAAWLNKTSEDFDTVEYTNISNRLA
                     LVLMYHNLSTQAQLLYTVYLDQALDKQYADMAAELERKEGGFGIAPVLNPVDCSPKAG
                     DVFQAQILASPYLYSLGGDVHIKVNGAGIPCKYGLGWYEAPASPGRHTINVEMAKR"
     assembly_gap    22000..22040
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22177..>23213)
                     /locus_tag="PK198_02100"
     CDS             complement(22177..>23213)
                     /locus_tag="PK198_02100"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HRF37553.1"
                     /translation="LLGMVSAQAQEVAPVALVAASGKVVYSSGGAKQKLVPGAVMKSS
                     GTLTLGKNGSALLLSDGRFKQLSGKQSWVLASQFQASAEGVSRLSFDQTFSNYVMSAL
                     QLAASPENAGDAWGAVRTGTGTGDGWGSVRTGTGTGDGWGNVRTGTGTGDGWGVVRTG
                     TGTGDGWGGKGNSIRAIRPFGKVLPGTATFRWSKPAGNRSFKLIISDMRNQTVLETTT
                     VDTFFTVDLTQPPFQAGVTYQWSIASNESPAAVSNTLQIELSDAAGRKAAIERVQRAA
                     SHAQNPPEVQMLMQAVSLEQADFFEEAAGIYSTLQKSQPKNQLARLMHAAFWMRYGLK
                     EVAREAYHKN"
     assembly_gap    23214..23230
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23333..>23734)
                     /locus_tag="PK198_02105"
     CDS             complement(23333..>23734)
                     /locus_tag="PK198_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37554.1"
                     /translation="PPRDVAAPSLERIDNLAIEDFLRSPEVDKEIRVAKQVVCGKKTW
                     ADSALWLHYEDDGQQYAVVHWCEGTGKNQTANSIGAGATSAAVLEAYGAPQRVLPDRG
                     GSLWVYPGLGILVAVSPQGQVAGWGVFRKSK"
     assembly_gap    23735..23890
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23891..>24135)
                     /locus_tag="PK198_02110"
     CDS             complement(<23891..>24135)
                     /locus_tag="PK198_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37555.1"
                     /translation="HSRLHSLKSVQSDRLEVRKALLDEAIQQFDRAILLDPKYAPAYL
                     HKACVYAVNGEWEEAAFWMKKGRRLAAAPQVAGFEVL"
     assembly_gap    24136..24158
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24159..>24508)
                     /locus_tag="PK198_02115"
     CDS             complement(<24159..>24508)
                     /locus_tag="PK198_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37556.1"
                     /translation="ADYMGGVLAFSAGYDVYGVMPRLLEQIYRSYGLPDTLKGYPSLP
                     ERVAVSQDAMRRLLDLQTVFETARYLSILENYESAAQYHRYILQDFQSREIYNNAGVN
                     LALAALPYFSTAEL"
     assembly_gap    24509..24521
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24522..24981)
                     /locus_tag="PK198_02120"
     CDS             complement(<24522..24981)
                     /locus_tag="PK198_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37557.1"
                     /translation="MLKKTLISAGLLFILNAMCAQEEHAFAFVLNPSGSAGLQAAAAY
                     KFEVANKVYKDLLQARGDARQQSPELVMTNGARYVAWMDPKKIRIGMEEKAYDVCVQF
                     GPDSLAAMAALLAHELTHYYEKHDWNRNFAHSHSGLKAAKAIGEIDEGIKQ"
BASE COUNT         5603 a         6381 c         6512 g         6167 t
ORIGIN      
        1 cttatctaat cctttggcct aaagaaaatg aacactttat ttttgcccca ctacttcgta
       61 gtgtgcgccg gcaggtttgc tgttttaaac cttacttttc agacagccaa aacatggcag
      121 ggaaaatctc ctatccaacc ttcatgctcg aataagggag tgaaagccag ccccagaaaa
      181 acgcccccgc caccggcgcc gcaaagtttg agcgaaaaag cgccccttgc attgccttcc
      241 tgccatattt gcaacacctc atcgggaatc atgggcgcca ggtgttgtgc ctgaaaccgg
      301 ctgatgcact gaaaatgccg gctgaaatcc tccatgcgat gatccaacca ggcagacacg
      361 gcagcatcag aaacatcaag cagttcggtg cgcatcagtg tttcaaaggc ggggtcttcg
      421 cagcgctgcc ggaaatactg caccataggc ccggtagagc gcgtgcggcg gctgtcgagc
      481 aaaaacaaag tcattcccgg cggcaaagca ggcacttgta ctgcatctaa ggcgccgtcg
      541 gggcgcagca acagaggctg attcagatag gataccaatg gatcaatacc agaactggcg
      601 ccgtgaaaga agctctccat tgccgccagg atgttacgga gttcgggcag gagcgaggca
      661 tcatggcgcg ctatgggctg attggcatag cgttcgtagg cagcagcaca cagggcgccg
      721 gaactaccca ggccgtatcc ggtggggatg tcggactgaa aaaacaagcc tgcttcgagg
      781 tcgtctttaa aagcggcaag gcggaaatcg caaggcaatt gtagcagata ctccacccat
      841 tcgcggaggc gaaattgttg gccggaatca gagccgaatt gccattggcc gccatacaaa
      901 tgcaagggtg cagcgagcgc cgccgcgccg cgcagcagaa tatgctcgcc gaacaaaaga
      961 actttggcag ggtatgtgcg catggtgtga gattatgccc acaaaatgat cggcatccat
     1021 attttgttca ttattcggca gttgttctca tgctgtctcg gcagtgcaga attcgcgcag
     1081 ccaatccgcg caggcatcaa aatttttaaa caccaaatgg ttgggcagta tattaggaat
     1141 gagcttgatg ccctccaaca tggctttggg ctgttgctga agcccggtca tggcgactgt
     1201 gatgccttgc cgttgtaagt cgaggacagc ttcttccatg gccagcatac cggattggtc
     1261 aatatagggt acttttttca ttctaaaaac cacgactttc acgccgggca ttactttgga
     1321 catttcctgg aaacgagaag caaaaccaaa aaacaacggc ccgtccaagt gcttgatata
     1381 caccttactg cctacttttt ccagcaggcc gatctcgtcg ttccagggaa tttccgagga
     1441 ataacttgtc agaggcgccg atacggttcg atgttctacc acgtcggaaa tggctttcat
     1501 aaaaagaata gaagccagca acataccgat ggctaccgcc tccagcaggc cgacgaacac
     1561 cgtgagaaca agaaccgcca tcataataat ggcatcgctg cgagggatgg ccctgatatt
     1621 ccgtatgcct ttgtaatcta tgatgccgat acccacagtg agcaaaatgc ctgccagtac
     1681 cgctgtggga acctgtccta ccaaaggccc catgcccaac agaatggcca acaacagcaa
     1741 cgccgcgacg atgccggaga gccttgtttt gccacctgaa ttggcattga tgacggttcg
     1801 catggtagcg cccgcaccgg gcaatcctgc aatgaacgcc gccgccatat tgccgatgcc
     1861 ctgtcccacc aattcttggt tgctgtcgtg tttggttttg gtgatattgt cggctactac
     1921 cgaagtaaga agcgtgtcaa tagcgcccag cgctgccaac gtggctgcat attccaaaat
     1981 gagtttccag ttggcgggta ccagcaaggg ggcaaaaaaa tggaggtgca aggtgggtac
     2041 gccgctgggc attttgccgc cgctgctgtc gctgatgaga cccacctctc cgcgaaacac
     2101 gagatagccc agcagtgaaa ctcccagcaa agcaatgagc gtactcggca cggctttgat
     2161 gatgcgcgga gaaaaataaa ccaataccac ggcaataatg gacaacacta cggcctccca
     2221 gtcaataaca cggggcaaat cgtccaaact cttcaatacg gccaacgagc ctccggtagg
     2281 ggtcatcgcc ccgaaaagcg gaaaaatctg cccaatgatg ataatgaccc cgataccgct
     2341 cataaacccc gataccaccg ggtaaggaat gtagcgaatg tactttccga tacggaatac
     2401 gcccataagc acctgcaaca ggccggcaac aaaaaaaacg gccaatacta agggaatagc
     2461 ttcctgcaaa ctgcccgcac gttgagttga ttcggcaatg atgaccgcag aaacaactgt
     2521 catgggggca gtagggccgc tgatttgtac cggtgtgccg ccgaaaagcg ctgcaaaaaa
     2581 accgagcgca atggcgccgt acaaacctgc aatggcgccg agttcagttt gtgcaccgaa
     2641 cgccaaagcc agcggcagcg ccacgatacc cgcggtgatg ccgccaaaca ggtcgccgcg
     2701 caggtgtttg agatcaaaac ctgtgttgaa tttcatggtg gagactttgc agcaaaacta
     2761 aataaaaaag gagagatact attgtacctc tcctcaaaag cctcagtcac catgcgaaat
     2821 cggcagtata atggccttac agccttgccg attcgtaaaa aatgatcttt ggaaaaagat
     2881 tactcacaaa taaacgatag cattacaatc tttattcaaa gcattgatgc cataacaaca
     2941 aggtaagtga cagattgttc ccggaaaatg cggagtaagg ccaacaaagg tctgttggca
     3001 ggcattttct tcttatctca tggaaggggc tcccttggat ggatgcttgg ctatttcaca
     3061 aaatacactg ttggctgtat tttgaatatc tggtcggggg tgctgtttcg ttcgtaatca
     3121 gtaacggatt ttatcaatat tttttaacaa gattaaatcg cctttatcga gtgagtttat
     3181 cttcgcttta atttctttgc tcagatttcc attggtgaca aactcgaatt ctcccttctt
     3241 ttttgatcgt attatcaatt tcatttcttc aactaaaaag ataatttgat tacgactata
     3301 taccattgtc gagaatcctc gtaaatcttc gagcctgtct ataggtattt cgttgtcagt
     3361 catttgatac tcctgagaat aaaaaacagg gatgattaca ggttgtaccg aataaacgac
     3421 aaaaaacagc tctgaatgaa tcttctttga tttctcctta ttgtaaatgg agattacaca
     3481 atcccccata gaattgggtt ttatcatgta tttacctttg gagaaccttt ggatttctcc
     3541 attgttcgtc tccacatgaa tgccttccct tccaaacccg ctttcatcta tttcaaattt
     3601 attctctgta tgaatgtata gagtccttcc ttcagaagtc tttatccggc aggaataatc
     3661 ttgtccctgg gctgctccaa aacataagag taccagcaat gacaacaaca ttttaatcat
     3721 ttgacatgtc tttgttgatg tgaattaata ccacaacccc gctctaaaag gcaaagcaag
     3781 ccaaacaggc gatatcccca ccacttaatt ctgttggaga atgtacactt tgtgtttcag
     3841 cccccgccaa tcaccccagg ctacgatgcc tgagcgtata gaaaaatatg gtgctgtagc
     3901 caaaggccaa catgaggtga aacaccagaa aggtagtgtt ttgaacataa aacgccagtg
     3961 ccaccgcccc cagaaagtac aaggccaaca agccctcgcc gatggtggtc cattccaatt
     4021 tgcgcatcat gtacttgcct ttgacgctgt cttgtttttt gtctaaaata ttgaatttgg
     4081 gggtgcgcac aaaggcagat tttttgccaa gatagccctg aagaacggcg cgggaattgt
     4141 gcagcgccat acccatagac agcgaaagga acagagggaa gtgcaaaatg aagcgataga
     4201 cggatttgga gaacggttcg tttttgcgct gcgcttccga ttgcaagttg gccacataat
     4261 agatggatat gatggacaac atgccgatca taaagtaagc aaaggaattt ttgttgaacc
     4321 cgcctgctat cagctcaccg aggaaataca gcagcggcac actggcaatg gaggccgtga
     4381 gtacaaacag gaaaatggtg ctgcccagca ggtgcatggt agcatggaat tttttccagc
     4441 gcgacagcgg cgaatgccat actaccggca gcattttttt ggccgtttcg gcgccgcctt
     4501 tcatccagcg gaattgctgc gacttgaggg cgttcatctc taccggcaat tctgcgggcg
     4561 agccgatttc ttccaaaaac ttgattttcc agcctttgag ttgtgcgcga atactcaggt
     4621 cgaagtcttc agtgagggta tcggcctccc agccgccggc atcttcgatg gccttgcgac
     4681 gccatacgcc cgctgtgccg ttgaactgta gcaacatgcc gcctgccatg cgcccgcgct
     4741 gctctaccgt gaagtgtacg ttgagctgga gcgcctgcaa ttgcgtgata agcgagtagt
     4801 cttcgttgag gtgttcccag cgggtttgca ccacgccgat ctccgggtcg cggaaatgcg
     4861 ggatcgtgtt tttgagaaaa tccggacgtg gcagaaagtc ggcatcaaaa atggccatga
     4921 attcgccgcg cgccacctcc atgccgtctt tgagggcgcc ggccttgtag cctttgcgct
     4981 ctttgcggcg cacctgctct atctgcaagc ccttggcctt gtactcttcc actttgcggc
     5041 gcactacttc ctgcgtttcg tcggtagnnn nnnnnnnaag gacgtgaatt tcaaagcgat
     5101 ctgtcggata gtcaaactga gcaatgcagt caatgaggcg ctcggtgacg tacatttcgt
     5161 tgtacagcgg caactgaacc gtcacgaaag ggtattcttt gttgcgttcg tcctcgctca
     5221 tactcatatc ggcagcggag ggaaaggcag tagcaccgga atcatctccg gctaaggcta
     5281 actgggctac agccttctgc ttcacggcat tatcctgccc cggtgaggcg cctgcggcct
     5341 gttttttgct ttttttgtaa tggtacaaca gatcgaactg aagtacnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnngacattg tttgtgaagt tttactcttt atcgctcgct tagggcaata
     5521 ggcacgggaa gcgcaaaaaa cgtgtgtaat attttaaact cgttcatggg cgtttccaga
     5581 tgatcgccct catgtatcaa acgtttttaa gaccggaacc gctcatcggt actcgttttt
     5641 ttttacataa tgtaccaatg aaaattcgct gcaaaggtag ctcattccca atcatatatt
     5701 ttacgagaga ccgtgaaatt atttttgtag cagatgtaca tgcccagaaa tggactactt
     5761 gggcaactga atgcaaaacg ttgtgccttt gccttcttcc gaccatttga caaatatcct
     5821 gcctttgtgg tagtcttcga tgatgcgctt ggtcaatgac agccccaggc cccagcctcg
     5881 ttttttggtg gtaaagccgg gttcaaatac cgttttgaat ttggaggtgg gaatgccctt
     5941 gccggtatcg gtaatcaaaa tgctgacata gcggtcatcc tccgtcactt cggcgctgat
     6001 gctgccctgg ccgtccatgg catctaaggc attgcgcaac aggttttcga tcacccagtc
     6061 aaacagcggc ggattgagcg gcacggaaag cgcaggcgag gaagccgggt cgggaaaatg
     6121 gaagttgacc ctgcgggagg cgcgtttttg catgtacgag cggcattcct ctaattcgtc
     6181 ataaatattg accgtatcaa gtaccggcgc ggaacctatt ttggagaagc ggtcggcaac
     6241 gagttgcagg cgattgacat ctttgttcag ttccagcagt acgtcttccg tatcggtatg
     6301 ttcttccagc acgagttgca ggtgctctat ccatgccatg atggccgaaa tgggcgtgcc
     6361 gagttggtgg gcggtttctt tggccatgcc tacccacacg cggttttgtt cggcgcggcg
     6421 ggcattgctg aagcccacat agcccatcgc cacaaaggcc gcgatgagca ggagttggat
     6481 aagagggaaa aagcgcaatt gcttgaggat gttggattcg ctgtaatata gagcaaaatc
     6541 cagcccctga atgggcttaa ggtctgattc ctgcattttt ttgagctgct tctcccacga
     6601 ggcagggtcg tctgttccca tattgactgc atcctgaatc ataccgtcgc ttcccaccaa
     6661 tatcatagga atagttttat tcgactgtat tacatccaga taaaagttag tttccatgca
     6721 gctcaggcaa ggaatgcaat cttcgccagg ttctttggcc atttcttcct gtgctttgag
     6781 gatgatgttg actttgttgc gctcttcaag cgctaagcga ttggtgagca tggtggtgta
     6841 aaccagcgaa ataagcacga tgagcgcccc tgcaatgccg agatagagtt tccaacgaga
     6901 cttttgggtg taaatatcca tgcctgtttt ctgtctgtga gtggtgtgaa cgcaagcctt
     6961 aacgtattgc ccgaccatat tagtttaaca accaacacca attatcaaac aaccggcctg
     7021 ctgcttacgg ctcgcagctt gaagccattc ctatcaagct acccccccga aaatcaatac
     7081 cgatacgcca ccgcattgat gttcataccc gcgcccacag aggcaaagag caccagatcg
     7141 ccctcctgaa cgcggtgttc gcctaattgc ccccggcgca ccatgtcgta catagtggga
     7201 atggtagcta ccgagctgtt gccgaaatcg tgaatgttca taggcgccac ctgagcatcg
     7261 tactcgcgca cattgtacag gcggaacaaa cgccgaacga tggcgtcgtc cattttttca
     7321 ttggcctgat ggatgaaaat tttgctcacc tcgctgatgt gctgcacgcc cgctcggtct
     7381 aagcattctt tcatggcagt gggcacatga gacagggcgt actcggatat ttttcgcccg
     7441 tgcattttga tatacaggtg gttatcgggc gattccggct tattggtcgg ccccatgaag
     7501 aggtagtagg tatcttcctg agcgtgagag ataaccgccg atgcgatgat gccctttttg
     7561 ccctcggtag gctgatactc caacacagcc gcaccggcgc cgtcagagaa gatcatggcg
     7621 tcgcggtcgt gcgggtccac gatgcgggaa agggtttcgg aaccgatgac gagggcgcgt
     7681 ttggcaatgc cggctttaaa aaaagcatcg gcctgaatga ggccctgcac ccagccggga
     7741 caaccaaaaa tgacatcgta gggaacgcaa tgcacgtttt tgatgccgag cgaatacttc
     7801 actctggagg ctacgctggg cagaatgtcg gtttggaggg tgccgctgag aatatcgccg
     7861 aaattgtgcg ccacgatgat ctggtcaatg gtttcgggat caatgcctgc atcgtccacg
     7921 gcgcggcggg cagcaatggc ggcaatatcg gaactgcgca gttcgggttt ggcgtagcgt
     7981 cgctcgcgga taccggtgat gctttccaat ttatctacaa tctctttggt gggccggtct
     8041 atcggcgtgc cgtcgggatt gtaaaattca ttggtttcaa atgccgcgtt ggtaacgact
     8101 tcggtgggca ggtaggcgcc cgtgccgatg atgactgtgt gtgacatagg ctgtggagat
     8161 gtatgattga gccgcaaaga tagggaatgg ttttcgggtg ggaacggatg gatttcggat
     8221 ttctaatttc gaggaggaac aagtggattt cgggttcaag gttccgggtt ggaggtttcg
     8281 ggggggagtg aaggggtgaa tcggcaagtt ttagcggggc agtcacccct tcactaagct
     8341 ggaaaaaacc gcccgacaag tcagggcgta acccgaaatt acggcctgac ctgcccgaaa
     8401 tcctgttcat cttgtaaaat cctgtctaaa aaacaagacc cggcttctga cgctgaaaga
     8461 cgcaggtttt tatgatcgtt acgtccctct ccgagcagcg gaaatcacga aaatcataag
     8521 gatcagcgtc ggaaaataaa gcgagggttc aaagtttcgg gtgggagccg ccgctcgccc
     8581 attgctttac cccaaaaaac tgagcagcaa ccccgccacc accgctgagc cgatcacccc
     8641 cgccacattg ggcgccatgg cgtgcatcag caagtgattg ctggggtctt cccgtaagcc
     8701 ttccaattgc gctacacgag cgctatccgg cactgccgac acgccggccg cgccaatgag
     8761 cggattgatg cggtcttcag gtttcaaaaa caggttcatc actttagcaa aaagtacgcc
     8821 cccggcagta gaaacgccga atgcaaatgc tcccaatgca aaaatcataa ccgactgcgg
     8881 cgtcaggaat acgtcggcct gcgtagaagc gcccaccgta agccccagaa taatggtaac
     8941 aatgtctatg agggctgtac gcgaggtttc ggccaagcgc ttggnnnnnn nnnnacgccc
     9001 gattctttga gcaaattgcc gaaaaagagc atacccagca gcggcagcgc actgggcgaa
     9061 atgaacgtgg tgaggataag tcccacgatg ggaaacaaca ttttttcggt gcgactgacg
     9121 gcacgcggcg gcttcatgcg catgaggcgt tcgcggcggg tagtgagcag gcgcatgatg
     9181 ggcggctgaa tgacgggtac caatgccatg taagagtacg cggctatggc aatagggccg
     9241 atgaggttgc gcgcataaac ggggtctccg ccgaccgtct gaccaatttc acgaacgccg
     9301 ttggccaatc tcgatgccaa aaaaatagcc gttggcccgt ctgcgccgcc aatgataccg
     9361 atagcgccgg cctcggcagg atgaaagccc agtatgagcg cgccgagaaa agtagtaaaa
     9421 atgcccaatt gcgcagcagc gcccagcaac atgagccggg gattggaaat gagcgaactg
     9481 aagtcggtca tggccccgat accgagaaaa ataagcggcg ggtaaatgcc cttgacaacg
     9541 ccgtagtaca aataattgag tacgctgccg ggttcgtaaa tgccgatttg cagccccgcg
     9601 cccgcctgaa acggtatatt gccgatgatg atgcccgtgc cgatgggcac caaaagcagg
     9661 ggttcgtatt cgtatttgat ggccagaaaa atgaacagca agcccactgc aatcataatg
     9721 ccatgcccgg cagtgaaatt ggcaaaaccg gtgtagtgaa aaaacgtacc caccgcttcg
     9781 gacccctgat gcgtagtttc agactgaaca tcagcctgtt gctgatcgga gcgggcaatg
     9841 aggccatgca atgtcgccag caacgcaatg acaaagaaga tgacgcctgc ttttttcatg
     9901 atgcttccat ttcaatcaac acatcgtttt ggagtaccgc atcgccgggc ttaatccgaa
     9961 cagaaaccac cttgcccggc ttttcagcat ggatgttgtt ttccatcttc atggcctcta
    10021 ttttgagcag cgtgtcgcct ttgttcacca cagctccggg agcaaccaac actgcggtga
    10081 cggtaccggg cagcggcgat ttgacaggca cagtgtcggt gcttgtgctg cgactgatgg
    10141 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaat tcgacatgat
    10261 tgttttcggc agacagtatt tccacctcgt aatcatggcc cttgatgcgg aacttgtact
    10321 ttttcataaa aacctgattt atcggcgaat cggatggcgc ataccgaata ttttagaact
    10381 ccagggcgag taggtcttgg gaatcttgcg gatggtgatg cgcgtggact cttcatcatg
    10441 caactcattg aaatacaaat gcaaagccat tgctatagca gccgcttcat cgccttttac
    10501 gctcaggtct tcattgtgag caacgtggtg ccccaacttc tgaagccgtc tgcggatttg
    10561 aagatgcaag agcttaggca ggtagcgaaa cgcgtaatag agcagcgcca atgtcagcag
    10621 ggtgacgctg tatccaacga tggaaatgag tatgccgtaa gaaagttgtt ccatgataaa
    10681 gtgttttttg gttcgggttt catccgccga agccttggct aaggtgggtt tcgggttcaa
    10741 ggttccgggt tggaaccgcc taccgaacac tcgcacttgg caccgcctac cgcccactgc
    10801 ctactgaagg cacaccctac gcttagcacc ccgcgccacg gcgcggctac tgcctaccgc
    10861 ctaccggttt acaatggaat attcgcatgt ttcttcggcg gcagcacgtc ttttttgctt
    10921 gccaatgccc gcagcgcgcg aatgatgcgc ggacgggtgt tgcgcggctc aatgatatca
    10981 tcaatgtagc cgaaactcgc cgctttgaac ggattggcaa acttctcccg gtactcttcc
    11041 tctttggcat tgaaaaacgc agcccgctcc tccgggtctt tcaactccga caaagcctta
    11101 ctctccaaca cctcaatggc gccgtgcgct cccatcacgg caatttcagc gcccggccag
    11161 gcgtagttga tgtcggcacg gagttgtttg ctgcccatca cgtcatgcgc gccgccataa
    11221 gacttgcgca gtgtgatggt gattttcggc accgtagcct cgccgtaggc aaagagcagc
    11281 ttggcgccat gcgtaatgat gcctccgtat tcctgaaaag agccgggcag gaagccgggc
    11341 acgtccacca aagtgagaat aggcacattg aaggcatcgc aaaaacgcac aaagcgcccg
    11401 gccttgcgcg ccgagttgat gtcgagtacg ccggctaaga aattgggctg attggccacg
    11461 atgccgcagg gcataccccc gaatttggca aaaccgatga cgatgttttt ggcccaatgc
    11521 cgctgaatct caagaaattc gctgttgtcg gcaatgatgt gaatgatgtc tttgacgtcg
    11581 taaggctggt tggggtcgtc gggtatgatt tcgttgagtt ggtcgtccat gcgatccaca
    11641 gggtcggtac attctgtggc gatggggtct tcgaggttgt tctgtggcag gtattccaat
    11701 attttgcgaa tgaggagcag gccctcgtct tcgctctcgg ctttgaaatg tgccaccccc
    11761 gatttgccgg tgtgtatatc tgccccgcca aggtcttcgg tactgatgtc ttcgccggta
    11821 acggttttgg ttactttggg gccggtgacg aacatgtggc tcgtcttatc gctcatgatg
    11881 atgatgtcgg tgagcgcggg cgaatagact gcaccgccgg cacaggggcc gaaaatggcg
    11941 gaaatctggg gcaccacccc cgaagccatg atgttgcggt agaaaatttc agcgtagccc
    12001 gccagcgagt tgacgccctc ctgtatgcgg gcgccgccgc tgtcgttgat gcctatgacc
    12061 ggagcgccca ccttgagcgc ctgatccatg attttgcaaa tcttgagggc gaatgtttca
    12121 gagagcgaac ctccgaaaac cgtgaaatcc tgcgagaaga catacaccac gcggccgtca
    12181 atggtgcccc gaccggtaat gacgccgtcg ccgagatagg tttctttttc gagaccgaag
    12241 ttggtgctac ggtgggtcac aaacatatcg tactcttcga aggagccttc gtcgaggagc
    12301 ttctcaatgc gttcgcgggc cgtgtatttg cctttgctgt gttgggcggc gatgcgtttt
    12361 tcaccaccgc cgaggcgggc ctgctcgcgc agctctacca attgcctgat tttatcgtgt
    12421 ctgtccatgc ggtacaattt taacttcgca ctctataaat acgaagccgc tattgccatt
    12481 ccgccgcagc tcaaccaaca ggtcccgaaa ggaaaagaag gaaaccctta ctcttccttc
    12541 aggttctgaa tcttattgag gagcaaggcg ccgcggcgca tgaagtcctc gtgaaactgc
    12601 gtatattcgt tataaaccat catcaaacga gcagcgtcga aagcggctga tttggggatg
    12661 atctgacggg ccgcgatcgg atgaagccag tattcgagat tgttggaacg cagataggag
    12721 ttgtccaatt caaaattgat gaccaccgac aacttgttga gcgagaagag atactccgtt
    12781 tctttgttga gtaccttttg caggtcaagg atgttgtaac ggctgaaatt aaccccggag
    12841 cgctcgcaca ggaacacgtc gcgacgaccg tcaaaggtga tgatgtctct gaattggtac
    12901 ttcttgaggt ttttcatcag gaagttccgg atggccattt tggccgggtg gagttgcttg
    12961 aaagcgtatt tcaactccat ttcctccatg cggaaatcgt ctttatacgg tttgcgacgg
    13021 cgggttttga gattgaccac ctcttttgca cgaccgatgg tatgccccaa ttcattgact
    13081 ttgggatata cattgacgat ctcaacgttg gcgttgttca cccaactctc gaggaagatt
    13141 cggttggaac cggtttcaaa gactaaaagg gcaatttcgc tgatggcgta gaagtttggg
    13201 tatccgccgt agatgttgcc atactttaac tctaaaaaag caatgttagt gttcacggtt
    13261 aagtggattt ggcagttggt taatgatttt gggagacaaa ttatcgttgt tgtgtttcat
    13321 gtttgaccgt gcagagtgca atattaagcc ttattcgcta aaggatggca aaaatatacg
    13381 ctcttcttga atttttatct atccgagcaa actgtttgtg tctaaaaaca aaaaatttcc
    13441 attttcatag attttttcgt cgtcggcata gatcattcct ccgtttttca tgtcggctat
    13501 catgtcccaa tgcaccgccg actggttttt gccgccggtt tgcaggtaag actgccccag
    13561 tgccatgtgt accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnaaatg gatgacgccg ttcaccgagt cttccaccgg agaggtatag acctcgcccg
    13861 aaggcatgtt ggtttggccg tccgagttga tccacacgcg gccctgggta gagaagcgga
    13921 tgtctatgcc ctcgcccaca tagcgtatgt tttcgcggct gttcagcagg tccactattt
    13981 gctgctgatt gcggcgcacc gagagccagc tttccatcgg gttgtcgtcg tacaaacggc
    14041 aggcattata cacaaagtgc tgatactctt cgagcgacat gccggcttcc tgcgccccgg
    14101 catcggtggg aaagaggcag aaattgcgtt tcagcgcccg cgtggccgag cgccggaagt
    14161 actcctgttg catgggtttc agggcttctc ggcgcagtgc tccgttatcg ggattcaagc
    14221 cgttgtcttc gcgcaggttg aagggggcct tgatgaacag gtaggcttcg tactcgctca
    14281 tggcctgccg gtatgccggc gaaacgtgct gctgcgcaaa agccgatcct tcgttgatga
    14341 ggatgcggtt tttctctctg aaatccatgt ccacatccac cgcggcgcca gcctgcaaag
    14401 ccgtgcgata cacctcgcgg atgagcggtt cggccagcat ggtgctgttc acatacagtc
    14461 ggtcgccggg ctttacttcc atgcaataat gcacgagcaa ctcggcgtat ttggtcagga
    14521 tatttttcat agttcgttgt ttttcaattt tttacatttt aaactcaaca atttcnnnnn
    14581 nnnnnnnnnn nnngccacaa gctacaagcc cttagctttg ttttcccaaa gtagaaaatt
    14641 catcttcaaa agcagataga ggtattcccc catgagggaa atctaattct ttttgacaac
    14701 caagctaaag gcttgtagct aatagcttgt agcctttgga gttagtgata aacagttgtg
    14761 tatgcccatc acttccaccg cgccgcctga tacggatacc gcaccacata ttgctgcttg
    14821 atgaggcgcg tcatcttttc gcgcagttcg gccagcccct cttttgtggc cgccgacata
    14881 aaaacgttgt cttttccaaa catgtaccgc aggttgcccc gcaggccgcc ctctatttct
    14941 ttgcgggcct cgtcgtccac aaagggatcg taattgagcg tgcggtacaa gtctatttta
    15001 ttgaaaacca aaatagtagg tttgtccaaa gcatccaagc tgcgcagggt ttcctctacg
    15061 gtgcggatgt gatcctcgtg ttgggggtgt gcaatgtcca ccacatggat gaggatatcg
    15121 ctttcgcgca cttcgtcgag ggtggatttg aaactctcga tgaggtggtg cggcagcttg
    15181 cggatgaagc ccaccgtgtc ggacaacagg aagggcatgt cttcaaaaac gactttgcgc
    15241 acggtggtgt ccaaggtggc aaagagcttg ttttctgcaa agacctcgga tttgctcagc
    15301 aggttcatca gggttgattt gcctacgttg gtgtatccta ccaatgccac gcggatgagt
    15361 tcgccgcggg tttttcgctg cgttgcgctt tggcgatcaa tgacttgcag tttttccttg
    15421 agttgggaga ttttgtcctt cacgatgcgg cggtcggttt ctatctcctt ttcgccggga
    15481 cctctgaggc cgatgccgcc gcgttggcgc tccaagtgcg tccacaagcc gcgcagacgg
    15541 ggcagcatgt attgcaattg ggccagttct acctgtgttt tggcctgcgc cgtttgcgcg
    15601 cgccgggcga agatgtcgag aatgaggctg cttcggtcta tgatcttgac tttgagggct
    15661 tcctccagaa tattgacctg tttgccgctg aggtcgtcgt cgaggatgac catgttgacc
    15721 tgctcgtgcc gctctatgta ctcacggatt tcgtccaatt tgcctttgcc gatgaaggtg
    15781 cgcgggtcgg ggtgcgtcat tttttgggta aaccgctgaa tgcactcggc gccggcggtg
    15841 agggccagga attccaactc gtccaaatgc tcgttgacga gggcctctgt ttcattttgg
    15901 caaatgacgc ccaccaacac tgcgtattcg gtctcttttg caatgctttt attgtctttt
    15961 tctcctaatg tccactcttt tgccatgttc tgatttattt cggatttcta attccggatt
    16021 cccgactctc gatttcggat tccggattcc gggttgtata taaattctac gttttgcacc
    16081 ccgcgccacg gcgcggctac tgcctaccgc aaaccgcatc accgcaacca cccggccgga
    16141 ttcaaccgtt gtttctcgcg ccacacctcg aaatgcaggt cttcattgcc caatttgccg
    16201 atggcctgcc ctgcgcccac ctcgtcgcct ttgcctaccg agagggattc gaggttagaa
    16261 tagaccgtgt aatacgcgcc atgttgcacg atgaccatgt tttgatagcc cggaatgaac
    16321 tgcacgccta ccaccttgcc cgcatgtacg gcgctgaccg ccgaaccggc tgaagcgctg
    16381 atgtccacgc cgttgttggg tacggtgacg cctttgaggg tgggatgcgg ctgcggcccg
    16441 aatgtgcgac tgatgcgccc gccgcctacg ggccatgcca gggagccttt gcgcgccccg
    16501 aagccactgt tgtcggctat ggcagcggct ggttccgggc tgtcgtcgcg gcttgcgggg
    16561 gccggcgagg tgattttgcg gggcggcggc gcggtgcggg cctctttgcg tttttgggcc
    16621 atttcgttgc ggatgatctg ctcaatggcc gcgctgaatc gctcgcgctc tttttcgcgg
    16681 cgctccaaat ctttcaccaa acggccttca tctgctttga gcgactgcaa agccttgtct
    16741 ttattgacca tttcgccgcg cagcagttct gtttgctctt cgaccgaaac gatgaggttt
    16801 cgttgttcct gttttcgctt ttccaactgg gctattttgt tggagagcat ttcctgggtt
    16861 tcctctatca gctttgcctg gcggcgacga tagcgttcgt attggcgcac atattggcgg
    16921 cgtttgagcg cctgattgag gcttttggaa gacaaaataa aggccaaatg gctttgttgc
    16981 aggcgaagtc gccaggcagt tcgcagcatt tgagcgtact cgtttctcag gcgggctatg
    17041 tcttcgctga gtgcgcccat caccaattcg ctgcgggcaa tggcagaatc ggcatgtgta
    17101 atctcctcct gcatggtggc taccaactgc tgccgcttct tcacctgaga acgcagggcg
    17161 atgtagcgat ctaaagtagc cgctttgttt ttttgagttt cctgaagttt ctgattggtc
    17221 tgattgattt cttgaacaag tttcaaacgc ttgtcttcca attccttacg agactgcgcg
    17281 cctgcgttcg gcaggaggaa aaaccagcac aaaacacacc aaagccccca tgacaacagg
    17341 cccttatttg ccctgcacac gggtgtattt ttcaggtatt tcgaatttgt tgtcctttgg
    17401 tacattgatc tcgacgtctg aaaattctat gcctgccgac attttgcccg atgaagggct
    17461 gttcaattcc agcaagcgaa ggtaagaaaa cttgcgattt ccggcaagct ctccataatt
    17521 gctgagcgaa tatctcaccg attgtagttt tttggggtct tccaactcca tttgtgccag
    17581 tgtcaggtcg gttttgttga tctgaaaacg attttcgaag ccgccgccct gcccgctgag
    17641 gcggtagtgt tcggggcttt catcgagttg aaaaccggat gtttgtaaaa acaccgggtt
    17701 gccgagcagg agattttgca gggcgaccaa gtcagccggc acgccgtagg ttttttccag
    17761 gtagctgagc ggttcggcgt agtactcgtt gttgaggcga ttgatgatga aagcagagtc
    17821 tttgcgaatg agcgcccgcc cgccttcaaa gccgaatttt tttacagaaa tccagaccca
    17881 ctcgtctttt ttcatacgaa tgacggcatt ggctttgatg gactggctgc cgtcgttgag
    17941 gtccaatttg gctttggctt ccatccattg cacgtctatt tttcgggcgg ccatgagctt
    18001 gagcatggct tcgggcgtat tggctttcag gtcggttttg cccacttttc ggcccggccc
    18061 gcaggatgca aacaaaatga ttgcactgaa aatgagaagg atggaatgga aatttgcttt
    18121 gcgcattgga ttcaagattt ctaatttcgg atttctaatt tcgggttgga acgggctggc
    18181 tttatgccgt aagcctccaa ctgcaagccg gcttactgta cgcttcgcac cgcctaccgc
    18241 acactgcaca ccccgcgcca cagcgcggca caccgcctca ctcataccac ctgcgatctg
    18301 caattttttt gtccaacagc gaagaacgcc cccctttggc ctgtgctttg gcccagtagt
    18361 tgacagcccc gtcaatatcc cccgtttgat acaaaaggtc gccgtagtgc tccaatagaa
    18421 gcgggagttc ttcttcgttg gttttcaatg ctttggaaag gcggctttta gcccctgcaa
    18481 agtctttctt tttgagcaag gcccaaccca tggcatggtc aacggcaacg tttccaggcg
    18541 acagcttgtc ggcctgttga gccatttcca atgccttgtc ggtgtattcg ttgcgcgcca
    18601 gcaaggagag gctgtattgg gccaagagtt ctccggaggc cgggtactgt ttgacggcct
    18661 catcgaagag cacatcggcg gcagggtgtt ttttttgaac atgggcaatg cctgccttta
    18721 cgcccaacag cagggcggca gcgcgctgat ctttcccgat catcagcatg gcctgctcca
    18781 ccacatcggc tgcgtcggag ctgcggccgg tttccagcag cgcccatgcg ttgaaatagt
    18841 gtgccatagc gcggttgggg aaataatcga gcgcattttc ggacatcctg agcagagctg
    18901 tgtattggcc gttctcggca taaatggtca tggcctgctc ccacacggta aacacgcctt
    18961 tatccaagtc gagggcgcgc aggtatttgg ccaaagcctc ttttttcctg ccggtgtaat
    19021 agaacaaatc gcctgcggcg gccaagggtt ttgcttcgcc gggatgcgct ttttcgagta
    19081 tggaggtgag ttccagtacc acgtcggcca gttcggtgtt gcgggtgtcg gccacttttt
    19141 gaataaaagg aatgatcttg ccgatcttct gatcaatatt ggctgccgga ttttcaaaaa
    19201 ccggcttgag tgcgcgcagg taggcgatgt cgtcgttggc ggcgttgcgc ccgttgccgg
    19261 caagtgccaa ttgcgcccgc gagtcgttgg gggcgatgct caaaatctgg cggtattcgg
    19321 cctgtgcctt ggcggtatcg ccgatttgtt cataaaaacc agccaacagg tgtctgtatt
    19381 cagtgcgttt tgggtaggca gcaatgaggc gctggagttc ctcggcagct tttttgttgt
    19441 tgcccaagcc cagatacagg gagtgtttgc ggcgcaccga ctcctcgctg atgccgttgt
    19501 ttttttccaa ttcgtcatac accttgatgg cgcgatctat ggcgtcggct tttaccaaaa
    19561 agtaggccca gcgatagtag taggcatcgt tgtgggcgtc gtttttgaca agctgttcgt
    19621 acagcgtagc cgccgacaaa aacttgttcg tcttttgata cacatcggcc agaaactttt
    19681 tgtaccaatt gttttccggc gacagcgccg tggcagaaac catcagtttt tcagcctccg
    19741 catacttgtt ttgggcatcg tacacccgcg ccagttcgta catggcagcg tggtttttgt
    19801 tgtctttttt cagaagacct tccaacaaag gaacagcctt ttcatagttg ccgatggccc
    19861 gctcgcgaca ggcctcaaca aacatttttt ccaattcggc agccgcctga gtgatttgct
    19921 ggccccaggc actgcctgcg tgcaaggccc aacagaggct gatcagaagg accagccgtg
    19981 tcttagtttt gctcatcgtg tttattatta catcttggag tagtcgccta agcttatttc
    20041 cgacgcctct ccggagtagt caacgttatt tcctattaaa gagttgatta agttggcgtt
    20101 gtgtatcaca gtttcgcttt gcacaatgct gctgctgata acggagcgat gcaaaccgga
    20161 attgcttcct accgatacaa aaggcccgat gacgctgttg cgcaccaccg ctccggcgcc
    20221 gatggagcaa ggtggaatga tcacagaatc aatgacttct gcggattcgt ctatcaattc
    20281 ttcctcctgg tgtttgagtt ccaacatgcg gcgattggta gccagcacgt tgtctttgtt
    20341 gccacagtcg agccactcct cgatgttggc cggtcggaat cgcagcccct tttgtttgag
    20401 gccctccagc gccgtggtga gttgaaattc gcctttgtct ttgatgtcgt tgtcgagcat
    20461 gtaggccagc tcgtcgcgca gttgttggcc gttgcggatg taataaatgc ctacgatggc
    20521 gaggtcggag atgaagaccg gggatttttc tacaaaatct gtgatgacat tgtcctcgct
    20581 gactttcacc acgccgaacg acaccggatc gggtacgctt ttcacccaga tgatgccgtc
    20641 ttcgtcgggg tcgaaggcaa aggtagctct aaacagggtg tcggaaaagg caatcataca
    20701 gcggccggtc atgctttcgc gggcgcaata aatggcatgg cccacgccca gcggcgcatt
    20761 ctggcggtaa atacggccta ccgcgcccaa ctgccgggca atatcgcaga ggcggttttc
    20821 cacttcttgc ccgaaatcgc cgacgacgaa ggctatttct tcaatttttt ccggtaagga
    20881 ggcagctatg tcttctacca atcgttgcac catgggcttg ccggcaatgg gcaacagcgg
    20941 tttgggaatg gtgagggtgt gcgggcgcaa acgcgaaccg cgacctgcca tgggaatgat
    21001 taaattcatg aatgcgtgcg tgtgtcggag ggcaaaggta taaaatgaga tgtattttcg
    21061 gtgccttata tgagtgttga gggggaaatg agaaatttgc gacgacattc ggcactattc
    21121 gacatggata tcgtagtgcc atcttttaat atggctttga ccagaaaatt tattttatga
    21181 acagattcgt atttttcttt ctcattttca cagcaagctg ctctcaagat caagcgacga
    21241 aaaaccagat ggcagactta gactccgcat accaagaggt gctgatattg gcggaaaaaa
    21301 ccaatcagca ggctgaggca acatgcagga tgaaagaaaa tttcatacac gcccttcagc
    21361 ctttacccta tttgatggct gacataaaag ccgtaggcaa tttgcatacc gcgcttcaaa
    21421 cgcaattgaa tgcgacactt aacgcggcag aaaacttgcc tgctgattcc cccgattggg
    21481 ccgttcctcc ggcaactatt tccagtatcc aatcctcctt caaagcattt gaagaagcgg
    21541 ttcgggcgca ttttccagat gaggaagccc tgccagatac acccgctgca tggctgaata
    21601 aaaccagcga ggattttgat actgtcgaat acacgaacat atccaaccgc ctcgcactcg
    21661 tgttgatgta ccacaattta tccacacaag ctcagttgtt atatacggtc tatttagatc
    21721 aggcgctcga caaacagtat gcggatatgg ctgcggagct tgaacgcaag gaaggcggat
    21781 ttggcatagc ccctgtgctg aatcccgttg actgctcgcc taaagccgga gatgtctttc
    21841 aggcacagat actggccagc ccctatctct acagcttggg cggcgatgtg catatcaaag
    21901 tcaacggcgc cggcatacct tgcaaatatg gcttaggatg gtacgaggcg ccggcttctc
    21961 ctggccgaca tacaatcaat gtggaaatgg cgaagcgcan nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn ccgtacaaac gcaataccag tacacggttg agtaagcgga
    22081 gaactatttt caatctcagt atcaaccgat ggctgattac ttaacggaag gaacggaaaa
    22141 gctgaactgc ttcagaaaat gcccgtctta ggcgtgtcag tttttatgat acgcctctct
    22201 cgccacttcc ttcagcccgt agcgcatcca aaaagcggcg tgcatgagcc gtgccaattg
    22261 gtttttgggt tgggatttct gaagggtgct gtaaataccg gcagcttcct caaaaaagtc
    22321 ggcctgctct aatgatacgg cctgcatgag catttgcact tcgggcggat tttgagcatg
    22381 ggaggcggcc cgttgcacgc gttctatggc ggccttgcgg ccggcagcgt cgctcagttc
    22441 tatttgaagc gtattcgata cggcagccgg ggactcatta gaggcaatac tccattgata
    22501 ggtgacaccg gcctgaaaag gcggctgtgt caggtccacc gtaaaaaagg tatccactgt
    22561 ggtggtttcc agcaccgttt ggttgcgcat atcagagatg atgagcttga agctgcgatt
    22621 gccggcaggc ttcgaccagc ggaaggtggc cgttcccggc aatactttgc cgaaaggccg
    22681 tatggctcgg atgctgttgc ctttaccgcc ccagccgtcg ccggtacctg tgccggtacg
    22741 cactacgccc catccatcgc cggtacctgt gcccgtgcgc acattgcccc aaccgtcgcc
    22801 ggtgcctgta cctgtgcgaa cgctgcccca gccgtcgccc gtgcccgtac ccgtgcgaac
    22861 ggcgccccag gcatcgccgg cgttttcggg cgatgcggcc agttgcaagg ccgacatgac
    22921 ataattgctg aaggtttggt cgaaactcaa tcgggacacc ccttctgcgc ttgcctgaaa
    22981 ttgtgaagcg agtacccatg attgcttgcc gctgagctgt ttgaaccggc catcggagag
    23041 caatagggcg ctgccgttct tgcctaatgt gagcgtgcct gaagacttca taactgcgcc
    23101 gggcactaat ttctgtttag cgcctcccga actataaacg acctttcccg atgcggctac
    23161 caaggccacc ggagcgactt cctgcgcctg agcggagacc atccccaaca gggnnnnnnn
    23221 nnnnnnnnnn ccagattaat aattgatttt tcatgttgtc cgtatttgtg ttttaggaat
    23281 atttgctgcg atctgcgagc ctcaagccgc aggctgcaag cctcaagtca agctactttg
    23341 attttctgaa aacgccccag ccggccacct gcccttgcgg ggaaacggcc accaagatgc
    23401 ccagcccagg atacacccac agcgagcctc cccggtcggg caatacccgc tgcggtgcgc
    23461 cgtaagcctc caataccgca gccgatgtgg cgcctgcgcc gatgctgttg gctgtttgat
    23521 ttttgcccgt accctcgcac cagtgaacca ctgcgtactg ttggccgtcg tcttcgtagt
    23581 ggagccaaag cgccgagtct gcccatgttt ttttaccaca gacgacctgc ttggccaccc
    23641 gaatttcttt gtccacttcg gggctgcgga gaaagtcctc tatggccaga ttgtctatgc
    23701 gttccaggct tggagcagcc acgtcgcggg gaggnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn ccagcacctc aaaaccggca acctggggcg cagcggcgag tcggcggcct
    23941 tttttcatcc aaaacgcggc ctcctcccat tcgccgttga ccgcatacac gcaggctttg
    24001 tgcaggtagg cgggcgcgta cttcggatcg aggaggatgg cgcggtcgaa ctgctgaatc
    24061 gcctcatcga gtagtgcttt gcgaacttcc aaacgatctg attgcacgct tttgaggctg
    24121 tgcagacggg agtgannnnn nnnnnnnnnn nnnnnnnnca gttcggcggt actgaaatag
    24181 ggcagtgctg ccagcgccag gttgacgccc gcgttgttgt aaatttcccg gctctggaaa
    24241 tcctgcaaaa tatagcgatg atactgcgcc gccgattcgt agttttccaa aatactgagg
    24301 tagcgggcgg tttcaaatac cgtttggaga tcgagcagtc ggcgcatggc atcttggctc
    24361 acggccaccc gctccggaag acttggatag cccttcaatg tatccggcaa gccataagac
    24421 cggtaaatct gctccagcaa acgcggcatg acaccatata catcgtagcc cgccgaaaac
    24481 gccagcactc cgcccatata gtcggcctnn nnnnnnnnnn ncctgcttga ttccttcgtc
    24541 tatctcaccg atggctttgg cggctttgag gccggagtgg ctgtgcgcaa aattgcggtt
    24601 ccagtcgtgt ttttcataat aatgagtcag ttcgtgggcc aacagcgcgg ccatggctgc
    24661 cagagaatcg gggccgaact gcacgcacac atcgtaggct ttttcctcca tgccgatgcg
    24721 gattttcttg gggtccatcc aagccacata gcgcgcaccg ttggtcatca ccaactcggg
    24781 cgattgctgc cgggcatctc cgcgggcctg caacaggtct ttatatacct tgttcgccac
    24841 ctcgaattta tacgcggcag cggcttgcag ccccgcacta ccggacggat tgagtacaaa
    24901 ggcaaaagcg tgttcttcct gtgcgcacat ggcattgagg atgaacagca agcctgcgga
    24961 tatgagcgtt tttttcagca tggatgttgt tgtgttttta tgactactca gtcgccctgc
    25021 aaaaagctgc gagccgcgag ctatgagcca cgagtttgat ttccaaaaat aaaaaagggc
    25081 attttttcaa gatgagattt tgaaattccc caaatgaaaa atctaattta tgaacgagaa
    25141 aacaaactca tagctcgtga ctcatagctc gtagcttttg gagactcgct gaatagttac
    25201 taatctttta ctacctttgg ggaaattccg cttgtaatac gattcgttat ggatttccac
    25261 cgcattttcg atattttgac ctaccagcag gcgcggtatg ccaaaccgac ggctttttct
    25321 ttgcgtacct cacaggggca agagcattgg tccaccgccg acgccctgcg cttg
//
LOCUS       DAORLF010000012        25227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1069_length_25227_cov_2.207254, whole genome shotgun sequence.
ACCESSION   DAORLF010000012 DAORLF010000000
VERSION     DAORLF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25227
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_25227_cov_2.207254"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>214
                     /locus_tag="PK198_02125"
     CDS             <1..>214
                     /locus_tag="PK198_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37558.1"
                     /translation="VPGVNLRQCHLMGSRDAMALFYHRRPDASMFAHSAMVMGGSWER
                     FAPYPATMFGDVSAIMIEHLPWIARMN"
     assembly_gap    215..356
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <357..>602
                     /locus_tag="PK198_02130"
     CDS             <357..>602
                     /locus_tag="PK198_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF37559.1"
                     /translation="LEMSGKRNMLELWPRFQLYVHGGVSFSPYRSQFQDFFPSGEVEY
                     MEVYNASEGYFSAQDTANAEDGMLLLLQNGIYYEFLP"
     assembly_gap    603..718
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <719..1218
                     /locus_tag="PK198_02135"
     CDS             <719..1218
                     /locus_tag="PK198_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF37560.1"
                     /translation="WRYLPGDTIMFTSLHPYRIRVTGRTKQFVNAFGEEVMVDNTDRA
                     LAETCMATGAMVSEYTVAPVYLQGGQKGGHEWLIEFERLPADMETFNALLDSNLQKVN
                     SDYEAKRYGNIALERLMLQAVPQGTFYRWMRARGKFGGQHKVPRLANHREYVEEIKEL
                     LRESA"
     assembly_gap    1557..1599
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1600..3780
                     /locus_tag="PK198_02140"
     CDS             <1600..3780
                     /locus_tag="PK198_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37561.1"
                     /translation="SFRGYCFLDPNIIYYKTPSAPVFLSFESLTEAYGSRHQAQLNEN
                     VQEWRDRICNQATVQDIYALIYKSSLNDIQYLYTAAAGKSRALDAFQRGNSFSRYLVR
                     NKCVETIAYLDFAKRCEPHVVPAADAWSTPPRDTLAMKRLIEQARGQFLEVKSDYIRL
                     RYAYQMIRLAHYAKDYPMVVELYNYLMPKIDHDPSVVEYWIKGHLAGAYMALGRHVEA
                     SYLYAQVFDNSRGKAETAYRSFRIRSDEEWEQCLLMCQSDRERAALYVMRASAADSRA
                     VEEMEKIYELYPNNPHLEVLLVREIKKLEKDFLGLSFNDNRLHNKRYQNLPRPEAGKY
                     LIRLQDFVQKAAREKQVARPDLWKISEGYLELLGGNYYDAERTFRQAGEMVSNDTLRA
                     QLNVFRLALRISQWQDATDTLDQEVYAIRSKNPYYQAFEDFTDFLNDKMVQLYTAKGQ
                     AGKAFLVQYPLRDLKPNPRAQELEDLIALCRKLTPTRIEQLMVAKNDTATMLHDLLNI
                     KATMLMARGDPGAALETWKEMLPIDAETFGFFNPFPERLGDCVHCPIKDAGASLTKRQ
                     LAEKLLEMEFRANAGVPGADTLYYELGLAWYNMSWFGHAWRATDFFRSGGSMAVQRRL
                     QTKDFVMPHRYFHLGNRENMDCSKAQFYFEKARLLATDPEYAARAAFMAAKCERNIQD
                     VAGLPYGARQYQYFELLRTKYRNTEFHNRIIRECKYFATYSARR"
     gene            4180..4743
                     /gene="frr"
                     /locus_tag="PK198_02145"
     CDS             4180..4743
                     /gene="frr"
                     /locus_tag="PK198_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRF37562.1"
                     /translation="MQEEVNHYVDHARELMDKAIEHLSHELIKVRTGKASTAMLEGLM
                     APYYGSPTPIGQIANISTSDARTIVIQPWEKNMLGPISRAIMEANLGFNPQDDGQVVR
                     ISVPPLTEERRKDLVKMAKNLGEDAKVGVRNARRDAIEHIKKAIKAGYPEDSGRDKEK
                     VVQALTDKYIQQVDHLIEVKEKDIMTV"
     gene            4935..>7670
                     /locus_tag="PK198_02150"
     CDS             4935..>7670
                     /locus_tag="PK198_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37563.1"
                     /translation="MRALTVLMIAALTLGAVHSLEAQYFGRNKPNYEKFNFKVLETPS
                     FQIYHYMENPEKVRAFAQDAETWFKLHQAILDDTIRGKNPLILYNDHADFQQTNTIMG
                     EIGVGTGGVTEAFKNRVILPFAMSNQQTHHVLGHELVHAYQYNMILNGDSTSIRNLEN
                     LPLWMVEGLAEYLSIGSIDPNTAMWMRDAVLNDKVPTIRDLVKPDFFPYRYGQAFWVF
                     LTGMFGDEVIKPFFVGTAKYGFDNACLLILGMKEKDLSKLWVETLKSHFGKQIGEGKE
                     KAPGSEIISGKGPGRLNIGPVLSPNGKYVIFLSERDLFSIDLYVADANDGKILRKIGG
                     TMKDGHLDDISYIESAGTWSPKGDEFAFVGVSKGRNVLVVKNAENGKTTRTISIPGVQ
                     AFTNPAWSPDGRKIVVAGLVQGQVDLYAYELRTGKVEQLTNDEYSEIMPAWSADGTQL
                     IYSTDELSMRRGRTNGKWKHNIAVMDIAGGRVNQIDVFPGADNLNAQFDPNGDILFLS
                     DRDGFRNLYRYSVLDKKVFQLTNSKTGISGITPFAPAFSIDRKRNRVAYTHYMGGAYS
                     IYKARPEDFLNQEVDPNTVDFTAATLPRVNRQASRIVDPVLAQIGALEDNLFAAEMVE
                     RPYKAKFALDYAGGSAGVGMGTSNLFGATTGAAGGVDLLFSDMTGNNKIFSTLALNGE
                     LQDIGGVVNYLNSKSRIGWGVGISHIPAFFFEGYQLAIDTINLGGQLTEVLRESITAQ
                     RLFESRGGFSAQYPFSTTLRVEASTSFTRYGFSRRRYDSFYDAFGRFLGQDRERLEAP
                     EGFSLYNVGTALVGDNSAFGLTSPLAGQRFRVGVNQFFGAITFTNITADYRRYLRLKP
                     VTFAVRAMHDGRYGGDSQFFPLFVISPWYIRGYSDFLGATNTQEIISRYQLDVEDY"
     assembly_gap    7671..7680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7681..8012
                     /locus_tag="PK198_02155"
     CDS             <7681..8012
                     /locus_tag="PK198_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37564.1"
                     /translation="ASKVMVGNFEVRLPFTGPKRVALIPTSFLLSDIALFADAGFAFN
                     SLDQVFGNVEEALRPRVLASVGASLRINLFGAMIVEPYYAIPVGQTLAPGHTNKGTFG
                     VNLMPGW"
     gene            complement(8100..9881)
                     /locus_tag="PK198_02160"
     CDS             complement(8100..9881)
                     /locus_tag="PK198_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRF37565.1"
                     /translation="MKRFAISILILLYGMAAVQAQTEQAPSIHQEQLEQYKNMALSPE
                     DWQRRNEQELPAARPKTDDTCRLQKRVFGWHPYWSNGLESQYNWKMLSDLSYFAYEVN
                     ATTGMALNTYSWSSAAVVNQALAQGVRVHLCATLFSNHATFFGSPTARQTLINNLITL
                     VQQRGAHGVNIDFEAVPAAQSANLTEFIIALGTQLHAAIPGAELSIALPAVDWSTVFN
                     VSAMNPYVDIFIIMGYDYYWSGSSQAGPTDPLYSFGADYDRNHSRSISYYLHAGVPRE
                     KLLLGLPYYGREWETVSNQAPANTTGAFHSSRTYKTVRDNSATYSQGNYHPASTSNYY
                     VYPSGSLWRQCFINSEHTLGRRYQQVQQRGLAGIGIWALGYDAGYEDLWNLIRDNFST
                     CAMQACADTLYDSGGPAWNYYHNENTTYTIAPPGATSLSVQFHSFQLENGYDTLWIYD
                     GSSTAAPLIGAFTASNSPGAIQASGGSLTFRFRSDVGVSAPGWMATYECSTINSTEES
                     AAGAAPLRIFPNPASDEVTVEYAVVEPQTVRIDLYDATGRVLHSRSQMQESGQYQARI
                     ALPAVSGPLWLRVRIGEKYWTKCVVKR"
     gene            9997..10629
                     /locus_tag="PK198_02165"
     CDS             9997..10629
                     /locus_tag="PK198_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37566.1"
                     /translation="MTTPANFTYLIGLLLFGWGLNSCAPQTSDFVGLFKMQPLPDTLA
                     FAITDSDAAIGADTISNQLFFSQLPADMYADIEYVADSSSSVTLARGSFELSPPYLLL
                     WADVRYAWFQHQSLLLYDKEAKRITDRITVAELYGGESGQIITGAWLLNGNEIVQRQD
                     SHSSRLTETDEVEQSTERYVSGWKIEGGRFIEQPVQDSARWIREFPMEDW"
     gene            complement(10630..10977)
                     /locus_tag="PK198_02170"
     CDS             complement(10630..10977)
                     /locus_tag="PK198_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRF37567.1"
                     /translation="MSVVKEFNDYRALMNEKILAQDNKVLKRFFNLDTNAYQDGALDT
                     KTKELLGLVASMVLRCDDCIRYHLGTCYELGVTRDQVFEVFAIANLVGGSICIPHTRR
                     AVEYWEALEAEAQ"
     gene            complement(10987..11691)
                     /locus_tag="PK198_02175"
     CDS             complement(10987..11691)
                     /locus_tag="PK198_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRF37568.1"
                     /translation="MNDSYSLGRKVLLLFAFSAFFSTFAAAFSEAVVADSDSSSIAAK
                     APAGVDFALRNDIVGYAQNYLGLKYRYAGRAPETGFDCSGFTHFVMGTFGIKLSPSSS
                     QQATQGEPVKLSETQPGDLVFFRRSARSRVSHVAMVYSNDEKGIHIIHSTNSRGIVID
                     NLLESAYWRPKFFKARRVINVQLSEIKEVLDAFAECDLDELDEAEEILITPQAAEERY
                     VCIEPLPSLPFRSIHR"
     assembly_gap    12018..12125
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12126..12661)
                     /locus_tag="PK198_02180"
     CDS             complement(<12126..12661)
                     /locus_tag="PK198_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF37569.1"
                     /translation="MTVEWYIWMIVIAGSFFAGSINTLAGNGSAITLTILTELLGLPG
                     NQANGTNRVGILMQTAAGGIAFYRNGRLDLRRSEWYLIFSVIGAIAGTILAVSISNEQ
                     FRVVFRFLMVFLLFLILFKPERWLHETEANRRPNWWVAGPLFVALGFYGGFIQMGMGL
                     FFLAAMVLGARYSMIDANA"
     gene            complement(12658..13437)
                     /locus_tag="PK198_02185"
     CDS             complement(12658..13437)
                     /locus_tag="PK198_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRF37570.1"
                     /translation="MPDDIKAVLDAAVDRFLRPEFLADDPIAVPHRFSRLQDIEIAGF
                     WTALLAWGQRKTIINSAKRLMELMDQAPHQFIVQHQEQDRKRFLDFKHRTFNATDALY
                     FLEFLQHWYQHHDSLEQAFAVHLPPGSPHTGPALTGFHELFFSLPDFPERTRKHIATP
                     ARNSACKRLNMFLRWMVRPSDNGVDFGLWRQIKPSQLLIPLDVHVDRVARRLGLLHRP
                     ASDWPAVLELTESLRQFDPQDPVKYDFALFGLGVLEQNKIL"
     gene            complement(13606..14358)
                     /locus_tag="PK198_02190"
     CDS             complement(13606..14358)
                     /locus_tag="PK198_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF37571.1"
                     /translation="MYKGKKVVVVLPAYNAALTLEQTYREIPFDLVDEVILCDDASRD
                     NTAELARSIGIEHVLVHERNKGYGGNQKTLYDKALSIGGDIVIMLHPDYQYTPKLIPA
                     IVNIIGEDLYPVVLASRILGKGALLGGMPLYKYVANRFLTLFQNWVINAKLSEYHTGY
                     RAFSGEVLRRINYGANSDDFVFDNEMLSQIIYAGFQIGEVTCPTKYFEEASSINLRRS
                     IIYGLGVLRVSLVHRLQLWGLAKSKLYAPRGA"
     assembly_gap    14476..14699
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14700..16216
                     /locus_tag="PK198_02195"
     CDS             <14700..16216
                     /locus_tag="PK198_02195"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37572.1"
                     /translation="LRYFFDCQGEYQHLFEGLAVANDEEMMTRHQGKYPVIMLSFKDV
                     KMRTYTSAMQAMTKLLYEEWARHSSLAGIFEYDDHLANMAAMVRVGEVREFLFRDAVK
                     KLADLLHAHHGAPVLLLIDEYDTPLVEAWLKGYYDDMIEFIRPLLGGALKDNQSLFKG
                     VLTGILRVAKESLFSGLNNFISDAGLKVGRYSDKFGFTEQEVKTLLAHYSLNGREMDE
                     ITAWYDGYRYGGIPIYNPWSILNYVAEGGQSPSPYWVNTGGHDLLKRLFFNPQSGIKD
                     KLEELLRGDAIEIYVDEFLTFRGLESNPDAVVSLMYFAGYLRVVGEFRRGDRIIRELS
                     IPNREVKLAYEDTLMAWFNEDLSRAFNDRLLDALTTGDVLTFGEGLAKFVLKVVSFYD
                     TADNRTEHFYHAFLLGLLARLDSAYLIRSNGESGYGRYDICLIPKDASRKGIIMEIKA
                     VRSRRSETMPGRLAEARTQLLANRYETELLAHGVTDILRLAIAVEGKEVVVEQV"
     gene            16242..17027
                     /locus_tag="PK198_02200"
     CDS             16242..17027
                     /locus_tag="PK198_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRF37573.1"
                     /translation="MNQRIYQIDAFTDQVFAGNPAAVCPLEHWLDDGLMQQIASENNL
                     SETAFIVKNGEQYDIRWFTPTIEVDLCGHAALAAAYALLFCEQHPGDFIRFYSHRSGM
                     LTVQRQGDLLTLDFPADVPTQIQNLPAWGKGFDMQPMEAWQGKTDLLLLFESEAQIRE
                     LQPQFDIIGQWEVRGVIATAQGVSTDFVSRFFAPQSGVPEDPVTGSAHTTLTPFWGER
                     LQKKELSAAQLSARGGRLHCRWNDERVAISGQARLYLRGSIEW"
     gene            complement(17036..17704)
                     /locus_tag="PK198_02205"
     CDS             complement(17036..17704)
                     /locus_tag="PK198_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37574.1"
                     /translation="MLILHSYYTQNQFFMKNLLLIAAILLPAWAWCQPGSIIRLANPS
                     FEGTPRPGTLPDGWTDCGFSRQSPPDTQPGYFGVTRKAFDGNTYLGMVVRDDGTRESV
                     SQQLSDTLRTGRCYLMSAMLSRSNVFRSLTSSFIDSADFTVPTVLRVYGGTSSCSKLE
                     LLAETAPVNHFKWMEYEFILKPRSNYTHILLAAEYEDMNKRFYNGHILLDRLSDIEEV
                     VCDD"
     gene            complement(17804..18439)
                     /locus_tag="PK198_02210"
     CDS             complement(17804..18439)
                     /locus_tag="PK198_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37575.1"
                     /translation="MRIVLLIAAMTLSGMAWCQPGSVIRLVNPSFEGDAPGPGNVPGG
                     WANCGPEGESPPDTQPYCFGVTQPPHYGESYLGMVVRETGTAESVGQKLSSALKANNC
                     YQLTVWLSRSDALRSPTNSYNVRDSADFTTPAVLRIYGGTSLYNKVELLAETPPIDHP
                     DWKEYAFVLQPLKSYTYFFLAAEHREANVFYNGHILVDHLSNLKWTACDDW"
     gene            complement(18439..18621)
                     /locus_tag="PK198_02215"
     CDS             complement(18439..18621)
                     /locus_tag="PK198_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF37576.1"
                     /translation="MMALEIAREARQIHGGMGITNEYPVMRHMMNLETVLTYEGTHDI
                     HLLITGMDVTGLNAFS"
     gene            complement(18596..>19296)
                     /locus_tag="PK198_02220"
     CDS             complement(18596..>19296)
                     /locus_tag="PK198_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF37577.1"
                     /translation="LVMFPIYKFGSEEQRRKYLPKLGNGDFIGCFGLTEPDHGSNPSG
                     MVTNVKDDGDAYILNGAKMWITNSPLADVAVVWAKDEAGIVRGMIVEKDMKGFSAPEI
                     KGKWSLRASITGELVFEDVRVPKSNVLPNVQGMKGPLSCLSKARYGIAWGAIGAAMDC
                     YDSALRYSQERTQFGRPIGQFQLTQKKLAEMITEITKAQLLAWRLGSLANEGKATPAQ
                     ISMAKRDDGAGNSP"
     assembly_gap    19297..19308
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19309..19648)
                     /locus_tag="PK198_02225"
     CDS             complement(<19309..19648)
                     /locus_tag="PK198_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF37578.1"
                     /translation="MQDNSNGTMIAKKAKEDRFQGHDYYMLDELLSEDHLLARAAVRD
                     WVKQEVTPIIEEYSDKAKCPTHLFKGLAEIGAFGPSLPAEYGGGGMDEIAYGVIMQEL
                     ERGDSGIRSMA"
     gene            complement(19827..20684)
                     /gene="fdhD"
                     /locus_tag="PK198_02230"
     CDS             complement(19827..20684)
                     /gene="fdhD"
                     /locus_tag="PK198_02230"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRF37579.1"
                     /translation="MTAHPDAGLKTCEILRVGGGEAGVVSDVLAVEEPLEIQVACGPA
                     AARRRHSIAVTMRTPGHDFDLAMGFLFTESIIARAEEVTLLRYVQQPSEAAAPSNVLL
                     AELRPDVQVSEQLLSRHFYTTSSCGICGKASIDLVNLHTCFSLLPKEPVVPAEILHQL
                     PQALAGQQDVFSHTGGLHAAGLFDAEGSLLLLREDVGRHNAVDKLIGAALQQGQVPLS
                     QYLLLVSGRAGFELGQKALMAGIPMLAAVGAPSSLCVELAEAYGMTLIGFLRNGRFNV
                     YSGRQRVSA"
     gene            20765..21988
                     /locus_tag="PK198_02235"
     CDS             20765..21988
                     /locus_tag="PK198_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HRF37580.1"
                     /translation="MSCKIWMAAALALLLISCKRDRSSFFADSEPDTQTYNRIQEQLL
                     DARPGDTIRLPEGVFEFDRPLSLDGISRVTIAGAGMDKTVLSFKYQKTGAEGIKITAD
                     SVTLRDFTVQDTKGDAIKLQNCTGVTLIQLKTTWSDGPDASNGGYGLYPVACKNVLID
                     SCEASFASDAGIYVGQSQDVVVRNCYAYGNVAGIEIENCIRSEVYDNRAENNTGGILV
                     FDLPDLPQTNGHTCRVYRNIIKNNNHSNFAPEGNIVGIVPPGTGIILLAAKNVEIFDN
                     EITGHKTIGTAIASYQITKNTWADTTYNPFSFDITIYNNTYQRKKSLPDLSKDFGKMV
                     NILFAGKPQDILYDGIIDSTRTLGPNPMNLCIRQDTSQLRFANIDAAHDFDKVSKVIA
                     PYLLCEGRTWPKEEK"
     gene            22032..22667
                     /locus_tag="PK198_02240"
     CDS             22032..22667
                     /locus_tag="PK198_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRF37581.1"
                     /translation="MTVPPENYLLVPLSVGFAGSLSFGPINLCVVDTTVRHNLRAGIW
                     FASAAALVESLQASIALYFGEFYPLFLRKYPWVHLVILVFFVALGIAFLRRSSSGESK
                     VKEGTGRHFFHGLVIALMNPQGIPFWIFVLAYLQSAQMFEINSTVSQTGLILFLSGVV
                     LGKFAALSLFGLLSNVINRRTAFLQQWTDKITGGILIALGLLQSFQFFLSP"
     gene            22817..>23455
                     /locus_tag="PK198_02245"
     CDS             22817..>23455
                     /locus_tag="PK198_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF37582.1"
                     /translation="MNTDFLQQLGLAPHNPGTFTGMESFSGGEKLESYSPVNGALIGS
                     VTQTTREEYEQVIRASEAAFKVWREVPAPKRGEIVRQYGDLLREYKDPLGRLVSYEMG
                     KSLQEGWGEVQEMIDICDFAVGQSRQLYGLSMHSERPHHRMYEQWHPLGIVGIISAFN
                     FPVAVWSWNSMIALVCGDVCVWKPSEKAPLCGVACQLLFQRILKANNLPEGII"
     assembly_gap    23456..23507
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23508..24350
                     /locus_tag="PK198_02250"
     CDS             <23508..24350
                     /locus_tag="PK198_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF37583.1"
                     /translation="DTRVPLISATGSTRMGKIVGQTVAGRMGRTLLELGGNNAIIITP
                     SADINIVLTGALFGAVGTAGQRCTSTRRLIIHESIYEEVKEKLARAYGQLRIGNPLDQ
                     HNHVGPLIDTGAVKAYLHAISEIGREGGSMLVEGGVLDGPGFESGCYVKPCVAEARPE
                     MAIVQHETFAPILYLLKYKDLDEAIAIQNGVPQGLSSAIMTNDMREAEKFLSVAGSDC
                     GIANVNIGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINYSKTVPLAQGI
                     QFDL"
     gene            24990..>25227
                     /locus_tag="PK198_02255"
     CDS             24990..>25227
                     /locus_tag="PK198_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF37584.1"
                     /translation="MAKIISLLNHKGGVGKTTSSINIGAGLAQLGKRTLLIDLDPQAN
                     LTLALGVPRQPSSMYEAMRGEAELAPFTLKEGLDV"
BASE COUNT         5770 a         6792 c         6505 g         5453 t
ORIGIN      
        1 gtgccgggcg tgaacctgcg gcagtgccac ctgatgggca gccgcgatgc gatggccttg
       61 ttttaccacc ggcggcccga tgcaagtatg tttgcgcact cggcgatggt gatgggcggt
      121 agttgggagc ggtttgcgcc atacccggcc accatgttcg gcgatgtatc ggccattatg
      181 atagaacacc tgccctggat agcgcggatg aattnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg
      361 gagatgagcg gcaagcgcaa tatgctggag ttgtggcccc ggtttcagtt gtatgtgcat
      421 ggcggcgtca gttttagtcc gtacagaagt cagtttcagg attttttccc ttcgggggag
      481 gtggaatata tggaggtgta caacgcatcg gaggggtact tttcggcgca agacactgcc
      541 aatgccgaag acggaatgtt gctgctgctg caaaacggca tttattacga gtttctgccc
      601 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
      721 tggcgctacc tgcccggcga taccatcatg tttacgagcc tgcatccgta ccgcatccgg
      781 gtgacggggc gcacgaagca gtttgtgaat gccttcggcg aagaggtgat ggtggacaat
      841 accgaccgcg cattagccga aacttgtatg gctacggggg cgatggtgtc ggaatatacc
      901 gtggcgccgg tttacctgca aggcggacaa aaaggcgggc atgaatggct gatagagttt
      961 gagcgccttc cggcagacat ggaaacattc aatgcgctgt tggacagtaa tttgcaaaaa
     1021 gtaaactcag attatgaggc caagcgctat ggcaatatcg ccttggagag gttgatgttg
     1081 caggctgttc cgcagggtac cttttaccgg tggatgcgtg cgaggggcaa gtttgggggg
     1141 cagcataagg tgccgaggct ggccaatcac cgggagtatg tggaggagat caaggagttg
     1201 ttgagggaga gtgcgtaatc tgctgccgca gttaatcaag tttatcggct ttagaagggt
     1261 ttagggtttc gggtgggaac ggacaaattt cggatttctg aatttacagg ttggagcggg
     1321 ttttatttct gatttctgat ttttcggata ggaacgggct aatttctgat ttcctcacgt
     1381 tcgcggaaat tcgcgccttt cgctaaaatt cgcgttctga gttgcgccac gacgtaggcg
     1441 ggtttagggt tcgggttcat ttgcggaatg tcaaacaata taaagtagaa tcatctgtag
     1501 gtgtaaaaac atgaaataca ggcattatct ggtagtaatt tgttggttgg ctcttgnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gttttcgggg ttactgtttt
     1621 ctcgatccca acatcattta ttacaaaacc ccctccgcgc cggtcttcct ttcgtttgaa
     1681 tccctgacag aggcttacgg cagtcggcat caggcgcaac tgaacgaaaa tgtgcaggag
     1741 tggcgcgacc gcatctgcaa tcaggctacg gtgcaggaca tttacgcgct catttacaaa
     1801 tcgagcctca acgacataca atacctctac acggcagcag ccggcaaaag cagagccttg
     1861 gatgccttcc agcggggcaa ttctttttcg cgctatttag tcagaaacaa gtgcgtggaa
     1921 accattgcct acctcgattt cgccaagcgt tgcgaaccgc atgtagtgcc cgccgccgac
     1981 gcatggagca ctccgccccg cgacactttg gccatgaaac ggctgataga acaagcccgc
     2041 gggcagtttc tggaagtgaa atccgactac atccggctgc gctatgcgta tcagatgatc
     2101 cggctggcac attacgccaa agactacccg atggtggtgg agttgtacaa ttacctcatg
     2161 cccaaaattg accacgatcc gagcgtggtg gagtactgga tcaaagggca tttggccgga
     2221 gcatatatgg cgctggggcg ccatgtggag gcctcgtacc tgtatgcaca ggtatttgac
     2281 aacagtcggg gcaaggcaga aaccgcctat cgcagctttc gcattcgcag cgacgaagag
     2341 tgggagcagt gcctgttgat gtgccaaagc gaccgggaac gcgccgcgct gtatgtgatg
     2401 cgcgcctcgg ctgccgacag tcgggcggtg gaggaaatgg aaaaaatata cgaattgtac
     2461 cccaacaacc cgcatctgga ggtgctgttg gtgcgggaaa taaaaaaatt ggaaaaagat
     2521 tttctggggc tttcgttcaa tgacaaccgg ttgcacaaca agcgctatca gaaccttccg
     2581 aggccggagg caggcaagta cctcattcgt ttacaggatt ttgtacaaaa agcggcccgc
     2641 gaaaaacaag tcgcccgccc cgatctgtgg aaaatctccg agggctatct cgaattgctg
     2701 ggcggcaatt attacgatgc ggagcgcacg ttccggcagg ccggcgagat ggtgagcaac
     2761 gatacgctcc gggcgcagct caacgttttt cgactggcac tgcgcatcag tcagtggcaa
     2821 gatgcgacag acacgcttga tcaggaggta tatgccatca ggagcaaaaa tccgtattac
     2881 caggcatttg aagacttcac cgattttctg aacgacaaaa tggtgcaatt gtacaccgcc
     2941 aaaggccagg cgggtaaggc attcttggta caataccccc tgcgcgacct caaacccaat
     3001 ccgagagcgc aggagttgga agacctcatt gcgctgtgcc ggaaactgac gcccacccgc
     3061 attgagcaac tcatggtagc caaaaacgac accgccacca tgctgcacga cctcctgaac
     3121 atcaaagcca ccatgctcat ggcgcgaggt gatcccggcg ccgccctcga aacctggaag
     3181 gaaatgctgc ccatagatgc cgaaactttt ggctttttca atccctttcc ggagcggctg
     3241 ggcgactgcg tgcattgccc tataaaggat gcgggcgcct cgctcaccaa acgccaattg
     3301 gccgaaaagc tattggaaat ggagttcaga gccaatgccg gggtaccggg cgccgacact
     3361 ttgtattacg aactggggct ggcctggtac aatatgagtt ggttcgggca tgcctggcgc
     3421 gccaccgatt ttttcagaag cggcggcagt atggcggtgc aaaggcgttt gcaaaccaaa
     3481 gattttgtga tgccgcaccg gtatttccac ctcggcaacc gcgaaaatat ggactgctcg
     3541 aaagcgcaat tctattttga aaaagcccgc ctgctggcca ccgacccgga atacgccgcc
     3601 cgcgccgcct tcatggccgc caagtgcgag cgcaacatac aagatgtagc cggactgcct
     3661 tatggggcaa gacagtacca gtattttgag ctgctccgaa ccaagtacag aaataccgaa
     3721 tttcacaacc gcatcatccg cgagtgcaag tattttgcga cgtattcggc gcggcggtag
     3781 ctgtggcaag caaacgaacc aaggtccagg tcgtgttttg aaaactcctc aagtggtaac
     3841 gatacagtcg ccctgcaaaa agctgcgagc cgcgagcttc gagtcacgag tttgattttc
     3901 caaaatgaaa aagggccttt tttcaagatg agattttgaa attttcccaa atggaaaatc
     3961 taattttaca atgaataaac aaactcatag ctcgtgccac gcctcggcgt ggtcgtagct
     4021 tggggggact ggctgagtag taacctcaag tggaaaaatc agatttttcc acaaaaaaac
     4081 aagctcaaag ccgatacctt gaagcttgtg gtatttggat attgccaaat agttgccaaa
     4141 atccctatat ttgcgccgcc aaccaaactg ctgtttatca tgcaagaaga agttaatcat
     4201 tatgtggacc atgcccggga gttgatggac aaagccatcg aacacctttc gcatgaattg
     4261 atcaaagtgc gtaccggcaa agcctctacc gccatgctcg aaggtctgat ggcgccttat
     4321 tacggttcgc ccacacccat tggtcagatc gccaacatct ctaccagcga cgcccgtacc
     4381 attgtcatcc agccctggga aaaaaacatg ctggggccga tttcccgcgc catcatggag
     4441 gccaacctcg gtttcaaccc gcaggacgac ggccaggtag tgcgcatcag cgtgccgccg
     4501 ctcacagagg aacgccgcaa agacttggta aaaatggcca aaaaccttgg cgaggatgct
     4561 aaagtaggtg tacgcaatgc ccgccgcgac gccatcgaac acatcaaaaa ggccatcaag
     4621 gccggttacc cggaagactc cggccgcgac aaagaaaaag tggtgcaggc gctcaccgac
     4681 aaatacatcc aacaggtgga ccacctcatt gaagtgaagg aaaaagatat tatgacggtg
     4741 tagcacaggt tgtcaccgtc tagcacccaa tgctcgtccg atcgaaattt ctggtcggac
     4801 gggcatttgt ttttttaaga catttttaac cccaaatgtc atttatccgg ccaattttca
     4861 gccgtctttt aaagtatgtt gttgttgtaa cgaaacacaa tttttattct atcccaaacc
     4921 aacacttgtg agccatgaga gcattaaccg tattgatgat tgccgcgctg acgctgggcg
     4981 ccgttcattc cctcgaagcg cagtatttcg ggcgcaacaa gcccaactat gaaaaattca
     5041 acttcaaggt actcgaaacg ccctctttcc agatttacca ctacatggaa aacccggaaa
     5101 aggtgcgcgc ttttgcccag gatgccgaaa cctggttcaa gctgcatcag gccatcttgg
     5161 acgataccat cagaggcaaa aacccgctga tactgtacaa cgaccacgcc gacttccagc
     5221 aaaccaatac catcatgggc gaaatcggtg tgggcacggg cggtgtcacg gaggcgttca
     5281 aaaaccgggt tatcctgcct ttcgccatgt cgaatcagca gactcaccac gttctgggcc
     5341 acgaattggt acacgcctac cagtacaaca tgatcctcaa cggcgactcc acctccatcc
     5401 gcaacctcga aaacctgccg ctgtggatgg tcgaaggctt ggccgaatac ctctccatcg
     5461 gcagcataga ccccaacacc gccatgtgga tgcgcgacgc cgtgctgaac gataaggtgc
     5521 ccaccattcg cgacttagtt aaacctgatt tcttccccta tcgctacggg caggctttct
     5581 gggtcttcct caccggcatg ttcggcgacg aagtcatcaa accgtttttt gtgggaacgg
     5641 ccaaatatgg cttcgacaac gcctgcctgc tcatcctcgg catgaaggaa aaagacctct
     5701 ccaagctctg ggtggaaaca ctgaagagcc attttggcaa acaaatcggc gagggtaagg
     5761 agaaagcccc cggttccgaa atcatcagcg gcaaaggtcc cggccgactc aacataggcc
     5821 cggtgctcag ccccaacggc aagtacgtca tttttctttc cgagcgcgac ctgttctcca
     5881 ttgaccttta tgtggccgat gccaacgacg gcaaaatcct ccgcaaaata ggcggcacca
     5941 tgaaagacgg ccacttggac gatatcagct acattgaatc ggcgggcacc tggtcgccca
     6001 aaggcgatga atttgccttt gtcggcgtca gcaaggggcg caatgttttg gtggtcaaaa
     6061 atgctgaaaa cggcaaaacc acgcgcacca tcagcattcc cggcgtacag gcattcacca
     6121 atcccgcctg gtcgcccgat ggccgaaaaa ttgtagtcgc cggcttggtg cagggccagg
     6181 tggacctgta cgcctacgaa ctccgcaccg gtaaagtaga acaactgacc aacgacgaat
     6241 actctgaaat catgccggcc tggtcggccg acggcacgca actaatctat tctaccgacg
     6301 aactgagcat gagacggggc cgcaccaacg gcaaatggaa gcacaacatc gccgtgatgg
     6361 acattgccgg cggacgagtg aatcagattg atgtctttcc cggcgccgac aacctcaacg
     6421 cacagtttga tcccaatgga gacatccttt tcctttccga ccgcgacggc ttccgcaatc
     6481 tctaccgcta ctcggtgttg gacaaaaaag tattccaact caccaactcc aaaaccggca
     6541 tcagcggcat cacgcctttt gcccccgctt tcagcattga ccgcaagcgc aaccgcgtag
     6601 cctataccca ctatatgggc ggtgcgtaca gcatatacaa agctcgcccg gaggactttc
     6661 tcaatcagga agtggatccc aacaccgtgg actttaccgc cgctaccctg ccccgtgtca
     6721 accggcaggc ttcccgcatc gtggaccccg tgctggctca aatcggcgct ttggaagaca
     6781 acctttttgc ggcagaaatg gtggaacgcc cctacaaagc caaattcgcc ctcgactatg
     6841 ccggcggcag cgcaggcgtg ggtatgggta ccagcaacct cttcggtgcc actaccgggg
     6901 cagccggcgg cgtggatttg ctgttcagcg acatgaccgg caacaacaag attttcagca
     6961 ccttagccct gaatggcgaa ctacaggata tcggcggcgt ggtgaattac ctcaactcca
     7021 aaagccgcat cggctggggc gtgggcatct cgcacatacc ggcattcttt tttgaaggat
     7081 accaattggc aatagatacc atcaacctcg gcggacagct taccgaggtg ttgcgggagt
     7141 ccatcaccgc gcagcgcttg tttgaaagtc ggggcggctt ttcggcgcag tatcccttct
     7201 ccaccacctt gcgggtagaa gcgagtacca gcttcacgcg ctacggattc agccgccgcc
     7261 gctacgattc attttacgat gctttcgggc gatttttggg gcaagaccgc gaacgattag
     7321 aagcgcctga agggttcagt ttgtacaatg taggtaccgc tttggtgggc gacaactccg
     7381 cattcggact cacttcgccc ttggccgggc agcgcttccg ggtgggcgtc aatcagtttt
     7441 tcggggccat tacttttacc aacatcactg ccgactaccg gcgttatctg cggctcaagc
     7501 ccgtcacctt cgccgtccgc gccatgcacg acgggcgcta cggcggcgat tcgcagtttt
     7561 tcccgctgtt cgtcatcagc ccttggtaca ttcgcggcta ctccgatttc ctcggcgcta
     7621 ccaacacgca ggaaatcatc agtcgctacc aattagatgt ggaagactac nnnnnnnnnn
     7681 gagcctctaa agtgatggtg ggtaattttg aggtcaggct gcccttcacc ggccccaagc
     7741 gggtagccct cattcctacc agcttcctgc tttccgatat tgccttgttt gccgatgccg
     7801 gttttgcgtt caacagcctc gaccaggttt ttggcaatgt agaggaggcg ctgcggcccc
     7861 gcgtattggc cagtgtaggt gcttcgttgc gcatcaatct gttcggcgcc atgatcgttg
     7921 agccgtacta cgccattccg gtgggccaga ccctggcgcc aggccacacg aacaagggga
     7981 cgtttggggt gaatttgatg ccggggtggt agggaattag aaaaaacaag aacgcccgat
     8041 atggagagta tttccatatc gggcgttttt ttaccccatc gtatgcgact ccctaccatt
     8101 caccttttca ccacgcactt cgtccagtac ttctcgccga tgcgcacccg cagccacagc
     8161 ggaccggaaa cggcaggaag cgcaatccgg gcttggtatt gaccgctttc ctgcatctga
     8221 gatcgtgagt gtagtacccg cccggtagca tcgtacaagt caatgcgcac ggtttgcggt
     8281 tccaccacag cgtattccac cgtgacctcg tccgaagccg gattgggaaa aatgcgcaat
     8341 ggggcagccc cggcagcgct ctcttcggta ctattgatgg tgctgcactc gtaagtggcc
     8401 atccagccag gagccgaaac gcccacatct gaacggaacc ggaacgtgag actgccgccc
     8461 gatgcctgta tggcgccggg gctgttgctt gctgtgaaag ccccaattag tggtgcggcg
     8521 gtgctgctgc cgtcgtaaat ccagagcgta tcgtagccgt tttcgagttg gaagctgtgg
     8581 aattgcacgc ttaatgacgt tgctcccggc ggcgctatgg tgtaagtcgt attttcattg
     8641 tggtaataat tccaagccgg gccgccgctg tcgtagaggg tatcggcgca agcctgcatg
     8701 gcgcaggtac tgaagttgtc gcgtatcaga ttccacaaat cctcataacc ggcatcgtag
     8761 cccaatgccc agatgccgat gcctgccagg ccgcgctgct gtacctgctg gtagcgcctg
     8821 cccagcgtgt gttctgagtt gatgaaacat tgccgccata ggctgccgct gggataaacg
     8881 taataattgg aagtactggc cgggtggtaa tttccctggg aataggtagc gctgttgtcg
     8941 cgtaccgttt tgtaagtccg tgaggagtga aaagcgccgg tggtattggc aggcgcctga
     9001 ttgctcactg tttcccattc gcgaccgtag taaggcaggc ccagcagcag tttttcgcgg
     9061 ggtacgccgg cgtgcaggta gtatgaaatg gagcgcgaat ggttgcggtc gtaatcggcg
     9121 ccgaagctgt acaaaggatc ggtggggccg gcctgcgaac tgccgctcca gtaataatcg
     9181 taccccatga tgataaaaat atccacatac ggattcatgg ccgatacgtt gaaaaccgtg
     9241 ctccagtcca ctgccggcaa tgcaatggaa agctcggcgc ccggaatggc cgcgtgcaat
     9301 tgagtgccca gtgcgatgat gaactcggtg agattggcgc tttgggccgc cggcaccgcc
     9361 tcaaaatcaa tgttgacgcc atgcgcgccg cgctgctgca ccagcgtgat gaggttgttg
     9421 atgagcgttt gccgggccgt ggggctgccg aaaaaggtag cgtggttgct gaacaaagta
     9481 gcgcacagat gcacccgcac accctgagcc aatgcctgat tcactaccgc cgccgaagac
     9541 cagctataag tatttagcgc catgccggta gtcgcgttca cttcatacgc aaagtaagat
     9601 aaatcgctga gcatcttcca gttgtactga ctttccaggc cgttgctcca gtaggggtgc
     9661 caaccgaaaa cgcgcttttg aaggcggcag gtgtcgtcgg ttttggggcg ggctgccggc
     9721 aattcctgtt cattgcgccg ctgccagtct tcgggactga gggccatatt tttatattgc
     9781 tccaactgct cctggtgtat cgagggcgct tgttctgttt gggcttgtac ggcggccatg
     9841 ccatacagca ggatcaaaat ggaaatggcg aatcgcttca tggatttgtt gtttggggta
     9901 aaaatacgaa accgccctga aaaacagtct gaacctgtat gattttggag caattcaatc
     9961 agaatccgac gcgtttttat acaaacattg ccaagcatga caacacctgc caacttcacc
    10021 tatttgatcg gattattgct gttcggatgg ggtctaaatt cttgcgctcc acaaacatcc
    10081 gattttgtcg ggctgttcaa aatgcagccc ttgccggata ctctggcttt tgccatcacc
    10141 gattctgatg ccgccattgg tgccgatacc atttccaatc aactcttttt cagtcaattg
    10201 cctgccgaca tgtacgccga catcgagtat gtagccgatt cctcttcatc cgtcacactc
    10261 gccagaggca gttttgaact cagtccgcca tacctgctcc tttgggcgga tgtacgctat
    10321 gcctggtttc agcatcagtc gctcttgctg tacgataagg aggccaaacg cattaccgac
    10381 cgaatcaccg tggcagaact ctacggcggc gaaagcggac agatcattac cggggcctgg
    10441 ctcctgaacg ggaacgagat cgtgcagcgg caagactcgc acagctcgcg cctgaccgag
    10501 accgatgaag tagaacaaag taccgagcgg tatgtgagtg ggtggaaaat agagggtggc
    10561 cggtttatag agcagccggt tcaggattca gccagatgga ttcgggaatt tccgatggaa
    10621 gattggtgat tattgcgctt ccgcctccaa tgcctcccaa tactctaccg cccgacgggt
    10681 atgtgggata cagatggaac ctcccacgag attggcaatg gcaaagactt caaacacctg
    10741 atcgcgggtg acgcccagtt cgtaacaagt gcccaaatgg tagcgaatac agtcgtcgca
    10801 gcgcagtacc atagatgcta ccaaaccgag taattctttg gttttggtat ctaatgcgcc
    10861 gtcttgataa gcattggtat cgagattgaa aaagcgcttg aggaccttgt tgtcctgggc
    10921 aaggattttt tcattcatca atgcccgata atcgttgaac tcctttacaa ccgacatgat
    10981 gcaggattaa cgatgaatgc ttctgaatgg gagtgaaggc agcggctcga tgcaaacata
    11041 gcgctcttcg gctgcctgcg gagtgataag tatctcttcg gcttcgtcta attcgtccag
    11101 atcgcactcg gcgaaggcat ccagcacttc cttgatctca gacaattgca cgttgatgac
    11161 gcggcgagcc ttgaagaatt tgggccgcca ataagctgat tccaacaggt tatcaatcac
    11221 gatgccacgc gaattggtgc tgtggatgat gtgaatgcct ttttcgtcat tggaataaac
    11281 catggccaca tgagagacgc ggctgcgggc gctgcggcgg aagaacacca gatcgccggg
    11341 ctgcgtttcg ctcagcttta ccggttcgcc ttgcgtagct tgttgtgaag aagatgggga
    11401 cagcttgatg ccgaatgtgc ccatcacgaa atgggtaaaa ccagagcagt cgaatcccgt
    11461 ttcgggagcg cgcccggcat agcgatattt taatccgaga taattttggg catatcccac
    11521 aatgtcattg cggagcgcga aatcaacgcc ggcaggagcc ttggcggcga tggaggagga
    11581 atcggaatca gcaaccacag cctctgaaaa tgctgctgcg aaagtgctga aaaatgctga
    11641 aaaagcaaac aatagcaaga ccttgcggcc taaactgtaa ctgtcattca tgagcgcgac
    11701 ctattaaaaa ttcaaaacaa tgttggcaaa ggtataaagc actttcataa aaccaaaaat
    11761 cttgccaaaa aaattgaaaa aagattgttg agggttttca cagcacttgt attataaaaa
    11821 agccaatgta tgccggtctt aaatatcttt tttgccaaat agtattctat tgccgtaccc
    11881 ctattgccac agtttttgca cctcaaacag ccgtgccaaa gacaccaaaa ccaccgccac
    11941 caacagcctg tgcgcccaca cattggcccc cggcaaacgc gaggcatagt gtgccgtgac
    12001 gtagccgccc gccgcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnngcatt ggcgtctatc atgctgtacc gggcgcccag caccatcgcg gccagaaaaa
    12181 acaagcccat gcccatctga atgaacccgc cgtaaaaacc cagcgccaca aacagtggcc
    12241 cggccaccca ccagttgggc cggcgattgg cctcggtttc gtggagccag cgttcgggtt
    12301 tgaagagaat gagaaaaagc agaaacacca tcaaaaaacg aaacaccacc cgaaactgct
    12361 cgttgctgat gctcaccgca aggatggtgc ctgctatggc gcctatgacg gaaaagatca
    12421 ggtaccactc gctgcgccgc aggtctaagc ggccgtttct gtaaaaggcg atacctccgg
    12481 cggcggtttg catgagaatg cctacccggt tggtgccgtt ggcctgattg cccggcaggc
    12541 ccaacaactc cgtcaggatg gtgagcgtaa tggcggaacc gttgcctgcc agggtattga
    12601 tggaaccggc aaagaaagag ccggcgatga cgatcatcca gatgtaccac tccacggtca
    12661 taagattttg ttttgctcca aaacgcccaa tccaaacagg gcaaaatcat atttcaccgg
    12721 gtcttgggga tcgaattgcc gcagcgattc ggtgagttcc agcacagccg gccagtcgga
    12781 agcagggcga tgcagcagcc ccagtctgcg ggctacgcgg tccacatgca cgtccaaagg
    12841 aatgagcagt tgcgagggct tgatttgccg ccaaaggccg aaatccacac cgttgtcgga
    12901 tgggcgcacc atccaacgca ggaacatgtt gaggcgtttg caggctgaat tacgcgccgg
    12961 agtcgcaatg tgcttgcggg tgcgctccgg gaaatcgggc aaagaaaaaa acagctcgtg
    13021 gaaaccggtt agcgcgggtc ctgtgtgtgg cgagccgggc ggcaaatgaa cggcgaaagc
    13081 ctgctctaag ctgtcgtggt gttgatacca gtgttgtaaa aactccaaaa aatagagcgc
    13141 atcggtggca ttgaaagtgc gatgtttgaa atcgaggaag cgttttcggt cctgctcctg
    13201 atgctgcacg atgaattggt gaggcgcctg atccatcagt tccatcaggc gcttggcgct
    13261 gttgatgatg gttttgcgtt gcccccaggc cagcagggca gtccagaacc cggcaatttc
    13321 aatgtcttgc aaacggctga agcgatgcgg tacggcgatg gggtcgtcgg ccagaaattc
    13381 aggtcgcaga aaacgatcta cggcggcatc caaaaccgct tttatgtcgt ccggcattcc
    13441 aaacaaatta aactgtgaga caaaaaatga atcaaaatac cgtttgcttt gcagttgttt
    13501 cctgaaaatc cgaaccaaat atgaccgcaa cgctcatcta cacactctct ttggccgtaa
    13561 tatttctatt gagaaaaatc atactcctct cgccgccacg ctggactacg cgccgcgcgg
    13621 agcatacagt tttgatttgg ccaggcccca aagttgtagg cgatgcacca aagaaacccg
    13681 cagcactccc aagccgtaga tgatgcttcg gcgcaggttg atggacgaag cctcctcaaa
    13741 atacttggtg gggcaggtga cttcgccaat ctgaaatccg gcatagatga tttgcgaaag
    13801 catctcgttg tcaaacacaa aatcgtctga attagcgccg taattgatcc gccggagcac
    13861 ctcgccgctg aaggcccggt agccggtatg gtactccgac agcttggcgt tgatgaccca
    13921 gttttggaaa agcgtcagaa aacgattggc cacatatttg tacaacggca tgccgcccag
    13981 cagcgcgccc ttgcccaaaa tgcgcgaggc cagcaccacc ggatacaggt cttcgccgat
    14041 gatgttcacg atggccggaa tgagtttggg cgtgtattgg tagtcggggt gcaacatgat
    14101 gacaatatcg ccgccaatag acaatgcctt gtcgtacagt gttttctgat taccgccata
    14161 gcctttgttg cgttcgtgta ccaatacatg ctcaatgccg atgctgcggg ccagttcggc
    14221 tgtattatcg cggctggcgt cgtcgcacaa gatgacttca tccaccagat cgaaggggat
    14281 ttcccggtac gtttgctcca aggtgagggc tgcattgtag gcagggagca ccaccactac
    14341 tttttttcct ttgtacatag gcgctgaatt gcggcgcaaa gatatttgtt ttcacaacat
    14401 cccaacgcag agagagaagt cgtgtagata gcagaaccta tcacttcaaa tggagacagg
    14461 atgagccggg acatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    14701 gctgcgctat ttctttgatt gtcagggcga ataccagcat ctttttgaag ggctggccgt
    14761 agccaatgat gaagaaatga tgacccggca tcaaggtaag tacccggtca ttatgctgtc
    14821 gttcaaggat gtgaaaatga ggacttatac ctcggccatg caggctatga caaagctgct
    14881 gtacgaggaa tgggcgcgtc actcctcttt ggcaggcatt tttgaatacg atgatcatct
    14941 ggccaatatg gcggcgatgg taagggtggg agaagtcaga gagttccttt ttcgggatgc
    15001 cgtcaaaaaa ttagcagacc tgctgcacgc acaccacggc gccccggtgc tgttgctgat
    15061 agacgagtac gatactcctt tggtggaggc ttggctgaaa ggctactacg atgatatgat
    15121 tgaattcata cgcccgctct tgggcggcgc gctcaaagac aaccaaagcc ttttcaaagg
    15181 cgtactcacc ggcatactac gagtggccaa agagagtctt ttcagtgggc tgaacaactt
    15241 catttccgac gccggcctca aagttgggcg ctactctgat aagttcggct tcacagagca
    15301 ggaagtaaaa accctgctgg cccattacag cctcaatggc cgcgaaatgg acgaaatcac
    15361 cgcctggtac gacggctacc gatacggagg tattccgatc tacaacccct ggtccatact
    15421 caattatgta gctgaaggcg gccagtctcc cagtccttat tgggtgaaca ccggcgggca
    15481 tgacctgctc aaacggcttt tttttaaccc gcagtcgggt ataaaagaca agttggaaga
    15541 gttgctgcgc ggcgatgcca ttgagatata tgtggatgag tttctcacct tccgaggttt
    15601 ggaatctaac cccgacgccg tggtcagtct gatgtacttt gccggctacc tgcgggtggt
    15661 gggcgagttc agaagaggag atagaatcat tcgagagctt tccatcccga accgagaggt
    15721 aaagttagct tacgaagaca ccctcatggc ctggttcaac gaggacttga gccgggcctt
    15781 caacgacagg ctcttggatg ccctcactac cggcgatgtg ctgaccttcg gcgagggctt
    15841 ggccaaattc gtcctcaagg tggtcagttt ttacgacacg gcagataatc gcaccgagca
    15901 tttctaccac gcctttctgc tgggcttgct ggcccgcttg gattcggcct acctcatccg
    15961 ttccaacgga gagagcggct acgggcgcta cgacatctgc ctgattccga aagacgcctc
    16021 ccgaaagggc attataatgg aaatcaaggc ggtgcgctca cgacgtagcg aaaccatgcc
    16081 gggccgcctc gccgaagctc gtacccagct actcgccaac cggtacgaaa cagaactcct
    16141 cgcccatggc gtgactgaca tcctgcgctt ggccattgcg gtggagggga aggaggttgt
    16201 tgtggaacaa gtttgatacc cttaatacac ctgccgccaa tatgaatcag agaatttacc
    16261 aaatagacgc cttcaccgat caggtttttg ccggcaatcc ggccgcagtt tgcccgctcg
    16321 aacactggct cgacgatggc ctgatgcaac aaattgcctc agaaaacaac ctctccgaaa
    16381 cggcttttat tgtaaaaaac ggagaacagt acgacatccg ctggttcacc ccaacgattg
    16441 aagtggacct ttgcgggcac gctgctctgg cggctgctta cgctttgctt ttctgcgaac
    16501 agcatcccgg cgacttcatc cggttttact cccatcgcag tggaatgttg accgttcagc
    16561 ggcagggcga cttgctcaca ttagactttc ctgccgatgt gccgacgcaa atccagaatc
    16621 tgcctgcctg gggtaaggga tttgatatgc agccgatgga agcatggcag ggcaaaacag
    16681 acctgctgct gttgtttgaa tcagaggctc aaatccgcga actgcaacca caattcgaca
    16741 tcatcgggca gtgggaggtt cggggtgtca ttgctacggc tcagggcgtt tcaacggatt
    16801 ttgtttcccg cttttttgcg ccgcaatcgg gcgtacccga agacccggtc accggctcgg
    16861 cccatactac gctcacaccg ttttggggag aaagattaca gaaaaaagaa ctctccgccg
    16921 ctcagctttc ggcgcgcggc ggccggctgc actgccgctg gaacgacgag cgcgtagcca
    16981 tcagcgggca ggcgcgattg tacctgcggg gaagtattga gtggtgatat cctcctcaat
    17041 catcgcacac cacctcctct atatccgaca agcggtccaa caaaatatga ccgttgtaaa
    17101 accgcttatt catgtcctca tattctgccg ccagcaagat gtgggtgtag ttgctgcgcg
    17161 gcttgagaat aaattcgtat tccatccatt tgaaatggtt caccggcgca gtttctgcca
    17221 gaagttccag tttgctgcac gacgaggttc caccatatac gcgcagtact gtcggaacgg
    17281 taaaatcggc cgagtctata aagctggaag taagacttct aaaaacattt gagcggctca
    17341 gcatagcgct catcagatag cagcgcccgg tgcgcaatgt atcgctcaac tgctggctga
    17401 cagattcacg ggtgccgtcg tctcgcacca ccattcccaa ataagtattc ccgtcaaaag
    17461 cctttctggt tactccaaag tagccgggct gcgtatcggg tggagactgc ctggagaacc
    17521 cacaatccgt ccatccatcg ggtaatgttc ccggcctggg cgtaccctca aacgacggat
    17581 tcgccaaacg aataatgctg cccggctggc accaggccca agcaggcaac agtatggctg
    17641 ctatcaacaa gaggtttttc ataaaaaact ggttttgagt ataataactg tgaagaatga
    17701 gcaagtttcg gagagttttg actggtctgc atggcatcgg atggcttagg ggcgataaaa
    17761 aaataaaatg agcatttatt tttgccccac tacttacgct gttttaccaa tcatcacacg
    17821 ctgtccattt taaattcgac aaatgatcta ccagaatgtg cccgttgtaa aacacattgg
    17881 cctccctgtg ttccgccgcc agaaagaagt atgtatagct cttgaggggc tgtaaaacaa
    17941 acgcatattc cttccagtca ggatgatcta tcggcggggt ttctgccagc aattccactt
    18001 tattataaag cgaagtgccg ccatatatcc tcaatactgc cggtgtcgta aaatctgccg
    18061 aatctcgaac attgtatgag ttggtagggc tgcgcaaggc atctgaacga ctcagccaga
    18121 ccgtcaattg ataacaatta ttggctttca gggcactgct tagcttttgt cctacggatt
    18181 ccgctgtgcc ggtttcccgc accaccatgc ccagatagct ttctccatag tgaggcggct
    18241 gagtcacccc gaaacaataa ggctgcgtat cgggcggaga ttcgccttcc gggccgcagt
    18301 tcgcccagcc gcccggcaca tttcccggac cgggggcgtc cccctcaaac gacggattca
    18361 ccaaacgaat aacgctgccc ggctggcacc aggccatacc ggacagcgtc atggctgcta
    18421 tcaaaagcac gatcctcatt acgagaaggc attgaggccc gtcacatcca tgcccgtgat
    18481 gagcaggtga atgtcgtggg tgccctcata ggtcagcacc gtttctaagt tcatcatgtg
    18541 gcgcatcacc gggtactcat tggtgatgcc catgccgccg tgtatctggc gcgcctcacg
    18601 ggctatttcc agcgccatca tcacgcttgg ccatcgaaat ctgagccggc gtggctttgc
    18661 cctcattggc caaggagccg agccgccacg ccaacagttg cgccttggtg atctcggtga
    18721 tcatctcggc caatttcttc tgcgtcaact ggaactgccc gatgggcctg ccgaactgcg
    18781 tgcgttcctg cgagtagcgc agcgccgaat cgtagcagtc catcgccgcc ccgatagcgc
    18841 cccaggctat gccgtagcgg gctttcgaga ggcaggagag cgggcctttc atgccctgca
    18901 cattgggcaa cacattgctt ttgggcacgc gcacgtcttc aaacaccaat tcgccggtga
    18961 tggaagcgcg caacgaccat ttgcctttga tttcgggcgc cgagaagccc ttcatgtctt
    19021 tctccacaat catgccgcgc acaatgccgg cctcgtcttt ggcccacacc accgcaacat
    19081 ccgccagagg cgagttggtg atccacattt tagcgccgtt gaggatgtac gcatcgccgt
    19141 cgtcttttac gttcgtcacc atgcccgacg ggttcgagcc gtggtcgggt tcggtgaggc
    19201 cgaaacagcc gatgaagtcg ccgttgccga gcttgggcag gtacttgcgg cgctgctctt
    19261 ccgagccgaa tttgtaaatc ggaaacatca ccaacgnnnn nnnnnnnnac gccatagagc
    19321 ggatgccgga gtcgccgcgc tccaattcct gcatgatgac gccgtaagcg atctcgtcca
    19381 tgccgccgcc gccgtattca gcaggcaggc tggggccaaa tgcgccgatc tcggccagcc
    19441 ctttgaacaa atgcgtgggg cacttggcct tgtcggaata ttcctcaatg atgggcgtca
    19501 cttcctgttt cacccagtcg cgcacagcgg cgcgggccag caggtggtct tcgctcagga
    19561 gttcgtccag catataataa tcgtggccct gaaagcggtc ttctttggcc tttttggcaa
    19621 tcatcgtgcc gttgctgtta tcttgcattt cgtccgtata tttggtgagc taaatgtggc
    19681 gcaaaggtat cgtttttttg gtcggatgcg ggagaaaatt gtgcagggaa aagggggcgt
    19741 gccggcgctc tcgcagatac ccatgcgccg tcgggtgctt cggtcgtcct cctatcgtcg
    19801 tccttccctt cgccccctca cgcaaactac gcagacaccc gctgccgtcc cgaatagaca
    19861 ttgaagcgcc cgtttctcaa aaaaccgatg agcgtcatac cataagcttc ggccaattct
    19921 acgcaaaggc tggacggcgc gcccactgcc gccagcattg gaatgccggc catgagcgcc
    19981 ttttggccca gttcaaaccc tgcccggcca ctcaccaaca gcagatattg cgaaagcgga
    20041 acctgcccct gctgcaaggc cgcgccgatg agcttgtcca ccgcattgtg ccgccccaca
    20101 tcttcccgca gcagcagcag gctcccctcc gcgtcgaaca aaccggcagc gtgcaggccg
    20161 cccgtgtggg agaatacatc ttgctgcccg gccagtgcct gaggcaattg gtgtaaaatt
    20221 tcggccggca ccacaggctc tttcggcaac aacgaaaagc aggtatgtag attcaccaga
    20281 tcaatggacg ccttgccgca aataccgcaa ctcgatgtag tgtaaaaatg gcggctcagc
    20341 aactgctcgc tcacctgcac atcggggcgc agttcggcca gcagcacatt cgagggggcc
    20401 gcagcctccg agggttgctg tacatagcgc aggagcgtca cctcttcggc ccgcgcaatg
    20461 atgctctcgg tgaacagaaa gcccatcgcc agatcgaaat cgtggccggg agtgcgcatc
    20521 gtcaccgcaa tactgtggcg tcggcgggca gcagcagggc cgcaggctac ctgtatttcc
    20581 aaaggctcct ccaccgccag cacgtcggaa accacgccgg cttcgccgcc gcctactctc
    20641 agtatttcgc aggtttttaa gcccgcgtcg ggatgtgctg tcatggttcg gagatgtgaa
    20701 aagacaaata tacgccctcc tcttgagaaa aaacgtgtac ctttcttcgc aaaagatgtc
    20761 gcacatgagt tgtaaaatat ggatggccgc agctttggcg ctgttgttga tttcctgcaa
    20821 aagagatcgc tcttcgtttt ttgctgattc tgaaccagat acgcaaacct acaatcgcat
    20881 tcaggagcaa ttgctcgatg cccggcccgg cgataccatt cgtttgccgg aaggcgtatt
    20941 tgagttcgac cgcccgctct cgctcgacgg catcagccgc gtgaccatcg ccggagcagg
    21001 catggacaaa acagtgctgt catttaaata tcaaaaaacc ggcgctgaag gtatcaaaat
    21061 taccgccgac tcggtcacgc tccgcgattt cacggtacaa gacaccaaag gcgacgccat
    21121 caaactccaa aactgcacgg gcgtcaccct gatccaattg aaaaccacct ggagcgacgg
    21181 ccccgatgca agcaacggcg gctacggctt gtatccggta gcttgcaaaa atgtgctgat
    21241 tgacagttgc gaggcttcat ttgcctcaga cgcaggcatt tatgtagggc agtcacagga
    21301 tgtcgtcgtc agaaattgct acgcttacgg caacgtggcg ggcatcgaaa tcgaaaactg
    21361 tatccgctca gaggtatatg acaatcgcgc agagaacaat accggcggca ttctcgtatt
    21421 tgacctgccc gatctgcccc aaaccaacgg ccatacctgt cgggtgtatc gcaacatcat
    21481 caaaaacaac aaccactcca attttgcgcc cgaaggcaac atcgtcggca ttgttccgcc
    21541 cggcaccggc atcattctgc tggccgccaa aaacgtggaa attttcgaca atgaaatcac
    21601 cggccacaaa accatcggca cggccattgc cagctatcag atcactaaaa atacctgggc
    21661 agataccacc tacaatcctt tttccttcga cataaccatc tacaacaata cttaccagcg
    21721 caaaaaatcg ctgcccgacc tgagcaagga cttcggcaaa atggtcaaca ttctcttcgc
    21781 cggcaaaccg caagacatcc tctacgacgg catcattgac agcacgcgca ccctgggacc
    21841 gaatcccatg aatctttgta ttcgacagga taccagccag ttgcgatttg ccaatataga
    21901 cgccgcccac gatttcgaca aagtgagcaa ggtcattgcg ccctacctcc tctgcgaggg
    21961 gcgcacctgg cccaaggaag agaaataacg cggatgcctc ttttacctct aaaaaaccta
    22021 cttttgcaca catgacagta ccacctgaaa actatctgct tgttccgctc agcgtaggat
    22081 ttgccggctc cctgtcgttc gggccgatca acctctgcgt ggtggacact accgtgcggc
    22141 acaatctgcg ggccggtatc tggtttgcat cggcggcggc attggtggaa agcctgcaag
    22201 cttccatcgc cctctatttt ggagaatttt accccttgtt tttgcgcaag tatccctggg
    22261 tgcatttggt cattctggtc ttttttgtgg cgctgggaat tgcctttctc aggcggagtt
    22321 catccggcga atcaaaagta aaggaaggca ccggcaggca tttttttcac ggcttggtca
    22381 ttgcactgat gaacccgcag ggcattccct tctggatttt tgtgctggcc tatctgcaat
    22441 cggcgcagat gtttgaaatc aacagcactg tgagccagac gggactgatc ctctttttga
    22501 gcggcgtggt attgggcaaa tttgccgccc tctccctctt cggcctgctg agcaatgtca
    22561 tcaaccgccg caccgccttc ctccagcaat ggaccgacaa aatcaccgga ggcatcctca
    22621 tcgcgctggg actgttgcag tcttttcagt tttttctcag tccgtaacga cggagcagtt
    22681 accttttttc tctccctgcc gaagcagccc gccctgttca actccgccct gtttttcata
    22741 cctttgcagc ccggaagtgc ccgcagcctg ttgcgcgcgc cgaaccatat tgctcaacta
    22801 tttcaaaacc aataacatga atacggattt tttgcaacaa ctcggccttg cgccgcataa
    22861 ccccggtact ttcaccggca tggaatcttt cagcggcggt gaaaaattgg aatcctactc
    22921 gcccgtcaac ggcgcgctca tcggctcggt gacgcaaacc acccgcgagg aatacgaaca
    22981 ggtcatccgc gcatcagaag cggccttcaa agtgtggcgc gaagtacccg ctcccaagcg
    23041 cggcgaaatc gtgcgccaat acggcgacct cctgcgcgaa tacaaagacc cgctcggccg
    23101 cctcgtttct tacgaaatgg gcaaaagcct ccaggaaggc tggggcgaag tgcaggaaat
    23161 gattgacatc tgcgatttcg ccgtgggaca atcgcgccag ttgtacggcc tcagcatgca
    23221 ctccgagcgc ccgcaccacc gcatgtacga acaatggcac ccgctgggca tcgtgggcat
    23281 catttccgcc ttcaatttcc cggtagctgt gtggagttgg aactccatga tcgcccttgt
    23341 ttgcggcgat gtctgcgtgt ggaaaccctc cgaaaaagcg cccctttgcg gcgtggcctg
    23401 ccagttgttg ttccagcgca ttctcaaagc caacaacctg cccgaaggca tcatcnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac acccgcgtgc
    23521 cgctcatctc ggccaccggc agcacccgca tgggcaagat cgtgggccag accgtagccg
    23581 gacgcatggg ccgcaccctg ctcgaactcg gaggcaacaa cgccatcatc atcacgccca
    23641 gcgccgacat caacatcgta ctcaccggcg cacttttcgg cgcagtgggc accgccggcc
    23701 aacgctgcac ttctacccgc cgcctcatca tccacgagag catttacgag gaagtgaagg
    23761 aaaaactggc tcgcgcctac gggcaattgc gcatcggcaa ccccttggac cagcacaacc
    23821 acgtcgggcc gctcattgac accggcgctg tgaaagccta cctgcacgcc atctccgaaa
    23881 ttgggcgcga aggcggctcc atgttggtag aaggcggcgt attggatggc cccggctttg
    23941 aaagcggctg ctatgtgaag ccctgtgtgg ccgaggcccg ccccgaaatg gccatcgtgc
    24001 agcacgagac ctttgcgcca atactttatc tgttgaaata caaagacttg gacgaagcca
    24061 tcgccatcca gaacggggtg ccgcagggct tgtcgagcgc cattatgacc aacgacatgc
    24121 gcgaggccga aaaattcctc tccgtggccg gctccgactg cggcattgcc aacgtcaaca
    24181 tcggcaccag cggggctgaa atcggcggcg ccttcggcgg cgaaaaagaa accggcggcg
    24241 gtcgcgaaag cggctccgat tcctggaaag cctacatgcg ccgccaaacg aatacgatca
    24301 attacagcaa aacggtgccg ctcgcgcagg gcatccagtt tgatttgtaa gcctgaaaac
    24361 tcattgcttc gattgcgaag cctttcagga ttcgtaatcg taaccttcgc tttcccgtta
    24421 cgtttacgaa tcctttttgt aggcttcgca aatgtacgga ccgctcccgc tgtgattcca
    24481 ttttcatgtt tacctgattg tgcctggatc agctcctgcc gagattccat ctcgtgcgag
    24541 tattatgcgg gttctacccg cctgtaaaac gcccgatttc agggcaatgt cgctctacgt
    24601 tttcctctac ggtcccctct acgtttgtca ttccgtaacg cagtggaggc atctgtacca
    24661 tctccgcaag cagccaagcg cggctgctaa gcgcggtggt gcagattcct acggaatgac
    24721 aaacggagag gggcgtagtg tggcaggcgg agagcgacaa acgtagaggg ttcgcagtgt
    24781 agccaacaca gcgcaacaat cgcagcccaa tcaaggaatc ttgcgccggc gctgtgcctc
    24841 tgcgaaaaga cgctgcatgg tttctttccg gtgatcttct gtcgccggta cgatccccgc
    24901 cgcgcatttt tttcctcctt ttccgcctgc atttcctgca aaagccgtta ctttaccgat
    24961 tcattgcaca agcaagaacc acatattgta tggcaaaaat catttcgcta ctcaatcata
    25021 aaggcggcgt aggcaaaacc accagctcca tcaacatcgg tgcgggcttg gcgcaattgg
    25081 gcaaacgcac gctcctcatt gacctcgacc cgcaagccaa cctcaccctc gcacttggcg
    25141 tgccacgcca gccttccagt atgtatgaag ccatgcgcgg cgaagcagag ctggcgccgt
    25201 tcactttgaa agaagggctc gatgtcg
//
LOCUS       DAORLF010000013        24872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1102_length_24872_cov_2.173027, whole genome shotgun sequence.
ACCESSION   DAORLF010000013 DAORLF010000000
VERSION     DAORLF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24872
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_24872_cov_2.173027"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /gene="dnaE"
                     /locus_tag="PK198_02260"
     CDS             complement(<1..1711)
                     /gene="dnaE"
                     /locus_tag="PK198_02260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRF37585.1"
                     /translation="MPEFVHLHCHTQYSLLDGATDIAAMMDKAKNDGQRGVALTDHGN
                     MFGAFKFVSEANKRGLKPIVGCEFYLVKDRHKKSFSRAKGEEDNRCHQLLLAKNKQGY
                     ENLSKLCSLGFLEGLYGKYPRIDKELLVQYHEGLIASSCCIGAEIPQAILHGKLDEAE
                     SLLRWWLDLFGEDFYIEIQRHRNMENIDGLGISQEDVNQHLLRLATKYNVKVICTNDS
                     HCLEEDDWRPHDVLLCVNTNSLIEQEDRFRFPSSDFYFKTQEEMNRLFHDVPHSIDAT
                     MEIFDKVDNLQLARDVLLPAFPLPAGFSSQDEYLRHLTFEGARKRYGTITPAIEERLN
                     FELEVIRNSGYPGYFLIVQDFTTTARQMGVAVGPGRGSAAGSAVAYCTGITNIDPIKY
                     DLLFERFLNPERVSMPDIDIDFDDEGRDKVIQYVIEKYGRNQVAQIVTYGTMAAKLSL
                     RDVGRVMNIPLSEVDRVAKTFPAHLKASLKKVLAPGDVDPKLKGDLNGDELEKAYKFR
                     ELAAGQDEIAEMIHTAEKLEGSIRNTGIHACGVVITPDEITKYVPVKIDKESDMLVTQ
                     FDNS"
     gene            complement(1776..>2377)
                     /locus_tag="PK198_02265"
     CDS             complement(1776..>2377)
                     /locus_tag="PK198_02265"
                     /inference="COORDINATES: protein motif:HMM:NF020786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37586.1"
                     /translation="IAKVLKIDVADLLGEDIPGNIAKESEVARDAPDVFNQIFPSLQM
                     LQDVAAQNGINDIFQDNGGKLLQVLLITGLKNLPGREGNDAVDAEGREYELKSVNVSL
                     TRSFSTHHHMNPTIIEKYRKVDWIFAVYRGIEILEIYKLTPGNLEPYFSDWEAKWHKD
                     GGKDINNPKIPLKYVRETGKLLFESDGTGKIRRINLSES"
     assembly_gap    2378..2408
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2642..3733
                     /locus_tag="PK198_02270"
     CDS             2642..3733
                     /locus_tag="PK198_02270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRF37587.1"
                     /translation="MKKAYSTQYGSQIIGNSLDVLQSYPDESIDLVITSPPFALLRKK
                     EYGNEDQAEYIGWLSQFAKLVYNKLKPTGSFVLDLGGAYKSGLPVRSLYNFRVPIEFC
                     DNIGFYLAEDFYWYNPSKLPSPIEWVNKQKIRVKDAVNNVWWFSKTPYPKADVTQVLA
                     PYSDRMKKLLDDPESYYTPKERPSGHNIGANFIDNGGAIPSNLLQIPNSESNSSYQAN
                     CKKAGLSAHPARFPSKLPEFFIKYLTEKGDFVVDIFSGSNTTGFIAESLKRKWNSIEL
                     DASYAANSSFRFLPKTLSEKDLLNVYFSILAGKEIPVMDFLMNGREVSELTKDFIHAE
                     KLHFELSGKLNKAERSKLQVIEKAAAVLF"
     gene            3772..5214
                     /locus_tag="PK198_02275"
     CDS             3772..5214
                     /locus_tag="PK198_02275"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRF37588.1"
                     /translation="MKKIILVALLITSATAARALEAGVSYCVFRSETGAYVELYLYVD
                     GASVEFAAGPDSSLQAGVEVVLYFKQQDQILRYDKFALHSPRMKQPEHFMDIKRYALP
                     DGDYELTAELRDLNRSGNEKTYQSPLSVRMSGNEILQSGIQLLAAARPDTSQNPFCKN
                     GIYMEPLARHTYGKTASELIFYHEIYHSDLALADDFLVTYRVETATADASRTVLIAHK
                     RRSPAPVVPLLLPMDISSLPNGDYRLVVEIRNRAQELKSRRESAFRRENPYLRSAALH
                     PDSVRIEDEFVEKLSADELRLSLRALTPQLAGKEQEALLLLIKSDSLKAQKNYLFRYW
                     IQKNANAPEAAYRQYMKVVMAVDQTFASGFRRGFETDRGHIYLKYGPPDDMETREQEP
                     SAPPYEVWSYNQFPATKQNNVRFIFYNPSLAPGDFVLLHSDAIGERQNPQWLRDLYRN
                     APGDWDSDPINGNDIRDGFNRNAKRVLRDN"
     gene            5323..5625
                     /locus_tag="PK198_02280"
     CDS             5323..5625
                     /locus_tag="PK198_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF37589.1"
                     /translation="MDIEVQELKAKMERGDSFLLLDVREPYEREEFNIGGEFIPMGMF
                     PRAAEHLEDHQNDEVIVYCRSGRRSATVQYMLQQAGFSNVRNLEGGVLAWIDAFGR"
     gene            complement(5641..6153)
                     /locus_tag="PK198_02285"
     CDS             complement(5641..6153)
                     /locus_tag="PK198_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37590.1"
                     /translation="MNSKQGNAAIITQICCLLDQLPQPAYTLPLEVLHGSSIGQHFRH
                     ILDFYQCLLRDIPAGTVNYTHRDRNPLMESLPEAAGEVFKELSDAIQALDESKPLSVL
                     GDFSAQDGDEQRPAFHSSVGRELMYAHDHAVHHLALIRIGIREAAPGIGFDPHLGYAP
                     ATVQHRAESC"
     gene            complement(6173..6802)
                     /gene="queE"
                     /locus_tag="PK198_02290"
     CDS             complement(6173..6802)
                     /gene="queE"
                     /locus_tag="PK198_02290"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HRF37591.1"
                     /translation="MSYKVKEIFFTLQGEGAQSGRPAVFCRFSGCNLWSGREEDRSKA
                     ICQFCDTDFWGTDGLNGGRYSAAELAQKVASLWPAPSGPRYVVCTGGEPLLQLDEPLI
                     AAFHAQGFEVAVETNGTIEAPAGLDWICVSPKAGSDILLRKGHELKLVFPQPDAEPPL
                     YEHWDFDCFYLQPMDGPEKEANTRLTLEYCMANPQWRLSLQTHKLLNIP"
     assembly_gap    6862..6892
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7078..9435
                     /locus_tag="PK198_02295"
     CDS             7078..9435
                     /locus_tag="PK198_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF37592.1"
                     /translation="MRKTLLLLLVAALPCILTAQKTTISGYITDGSSGEPLISANVFE
                     ARLEVGTVSNVYGFFSLTLESRDSAVLLFSYMGYQPQELRVALSANATLQIALQPFVE
                     LATVEVRADRSERIEQRTQMGRIEVPIEQIKRVPALLGEVDVLKSLQLLPGVQSGGEG
                     QNGLYVRGGSPDQNLVLLDGVPVYNVSHVLGIFSVFNADAIKNVTLTKGGFPARYGGR
                     LSSVLEINMKEGNTKAFHGEGSIGLISSKLTLEGPIIKDKTSFLISARRTYADLIFKP
                     IIKAVQPPDQKFDIALYFYDFNAKIQHKFNDRHRLYLSAYSGADVFQTTFREQNDAFS
                     GGLDWGNLISAARWNWQISPRLFANTTLTYSRYKIDILSAQESKFNNVVEKFSAKYIS
                     GIEDLGGKIDFDFIPNPRHYIRFGGGATNHTYNPGALALKEQSGGTTNIDTLLGSRKA
                     FSTEYFLYAEDDFSLGALKINAGLHASAFDVEGKLYTSLQPRLGLRYLMKNKLSLKAG
                     FSTMVQYINLLTSEALSLPTDLWVPSTRSVLPQQAWQVSVGAARTFWDEFEFSLEGYY
                     KKMSNVISFKEGASFLFGLENDWQDKVTQGEGESYGLELFLQKKAGKTTGWIGYTLSW
                     NWRQFDEINGGRRYPFRYDRRHDVSVVINHDFSKRVGLSAAWVYGTGNAVTLNTFRYP
                     GYIGQFTDIFGNTTDYVSDVESGGERNAFRMRDYHRLDISLELRKQRPKSERKWVFGV
                     YNAYWRRNPYFMTTGSVFENGVSRRVFREVSILPIIPSVSYQFKF"
     gene            9454..10365
                     /locus_tag="PK198_02300"
     CDS             9454..10365
                     /locus_tag="PK198_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRF37593.1"
                     /translation="MKYTAFIFALLSAITALTYSSCTEDSFSQIVEIDLPEHRPRPVI
                     ASQINAGDTLVQALVSISMGVLDTGNYVYPANATVRLLREGQEPLPLTFNPDKKSYTA
                     PAPAAGFVPGARYRIEAQVPGYGTAYSEQIMPGAPDVASVELRRNAVINPNGNRSDEM
                     LITIDDPAGSANYYRIELWQEGTLIFLDDTIEYNNPAYLESNDPNLQDGSSGGKVLAD
                     GAFDGTRYIVRFSTGRTWIDASNTVHKYTLRIAQITRDAYLYDRTITQYYRALGNPFA
                     EPVTVHSNIEGGYGVFRAAHWREWELR"
     gene            10509..11084
                     /locus_tag="PK198_02305"
     CDS             10509..11084
                     /locus_tag="PK198_02305"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRF37594.1"
                     /translation="MINLILFGPPGSGKGTQALKLTERYGLLHISTGDLFRYELGNNT
                     PLGVLARSYMEKGQLVPDEVTVGMLRNKVEANPDVNGYIFDGFPRTIPQAEALDALLT
                     ERNDSITKLIMLDVTEDEIVNRILERGKNSGRADDNDPAIIRNRFEVYLKETSPVYDF
                     YAQSGKSVKVHGIGGIEEIFEKLCAEIDAVA"
     gene            11081..12232
                     /locus_tag="PK198_02310"
     CDS             11081..12232
                     /locus_tag="PK198_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37595.1"
                     /translation="MSTAAPATAAQEPFLLKNVFNRELLEGYALVLSSVWPEFDTHRF
                     LNRLFDEAWPALELKERMRRISVVLREFLPPDYAEAVPILVATTRRMVAESGEKLTFE
                     YMFIPDFVQHFGQDHYEVSVPALGEITRWTSAEFAVRPFLLRYPDRMAAQMLVWSRHE
                     SPMLRRLSSEGIRPRLPWGMGVPALKKDPAPILPVLENLKNDPAETVRRSVANNLNDI
                     AKDHPDLVLGIARRWAGHSPETDWVVRHACRGLLKKGHPEALALFGFEQGVAGISVEA
                     LRADAEVRIGGRLAFAFELANTTSQPCALRLEYAINYCTLSGKTSRKIFKIKELEMNS
                     GERAAFARAQRFQDFTTRKHYPGTHQIDILVNGKVSASAAFEVISFLHG"
     gene            12241..13371
                     /gene="chrA"
                     /locus_tag="PK198_02315"
     CDS             12241..13371
                     /gene="chrA"
                     /locus_tag="PK198_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRF37596.1"
                     /translation="MEHKAALKDIAKLFLKLGIIGFGGPAAHIAMMQNEVVVKRKWLT
                     EQHFLDLIGATNLIPGPNSTEMAIHIGYEKGGWKGLITAGLCFILPAVFITGFFAYLY
                     KLYGQLPEVQPFVYGIKPAIIAIILGAIFPLAKKSFKSAELIFIGLLVLAGSLLGINE
                     IYLMFGAGFFALLLAYVRSKKSNHINGLLPFTLLQITNTTLLSASNANLFWIFLKIGA
                     ILYGSGYVLFAFLDTELVATGLLTRQQLMDAIAVGQFTPGPVFSSVTFIGYQINGWTG
                     AVISTIAIFLPSFVFVALLNPLVKRMRNSELFSAFLDAVNAASVALIAAVCFEMGKDA
                     VTDWRTILIAMASLSVAFGYRKVNSVFVVLGGSFVGYLLALI"
     gene            13425..15206
                     /locus_tag="PK198_02320"
     CDS             13425..15206
                     /locus_tag="PK198_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37597.1"
                     /translation="MLNKYRILLLIGSVLLPLASGFAQGSSLPLGSESYHIIDRLSIK
                     TGLYAPFHTNARPHPRGAVARLAMQADTADVELSDLYRKDLEYIFRDNNEWLGMASFA
                     TTLGGRKERAWPDTALTQVECSLLDARYARCRKPILGFAYPTPANFIEVNDKYFHLRA
                     NPMLNLHYAPLSNEEQPVFINQRGLELRAGVDDRIYFYANIVESQAALPQYVVQRVRR
                     DIALPGAALYKPYNSNVFNIQNGYDFLNGQGVLGFNISRHVGAQLGYGRHFIGNGYRS
                     MLLSDFAQNYPFLRLNWNVWKFHYQNLYAELDIRSANQTRDGTLVSKKYMEAHHLSIN
                     LGKNVNIGVFETVIFARPNHFALHYLNPVILYRAVEQATGSPDNVLIGLDGRWNIARR
                     FQLYGQFIFDEFVFRELVLENKGWWANKYGIQAGLKYVDVLGIDHLDVQAELNFSRPY
                     NYTHRDSTGSYAHHNQALAHPLGGNFKEAVFIARYRPMPRLFVEARLIRANIGEDAPG
                     QNWGNNILLNHDTRVQEYGNNTGQGIAADVTLAGLDISFMAAHNVFFDVQYFYRNKTS
                     EDPLRNNQVQYIGGGIRVNMAKLRMDF"
     gene            complement(15281..16339)
                     /gene="corA"
                     /locus_tag="PK198_02325"
     CDS             complement(15281..16339)
                     /gene="corA"
                     /locus_tag="PK198_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRF37598.1"
                     /translation="MSRRRKKTQRKHGLPPGTLVFTGDRHTDVPDIVLVQYTESDTFM
                     QSAREMAPPAREMAGVVWYDVRGLHHVPLIEQLGRQFGVHTLALEDVLDIQQRAKFED
                     YEDAVFIIAPALTFDEATLEVQHEQIGIYAGKDFVLSFQEKPDDTFASVRDRILHGNG
                     RIRKRGADYLVYALLDTIVDHYYLVLDKLEELIDNQEHQVLTAPDESTKRSIHHLKLQ
                     VLALRHTVFPLREAVNAFSRSDNPMVQDSTRLFIRDIYDHTIQIMDAIDTWRDVLNGI
                     FDLYLSQLSFRMNNVMQVLTIISTIFIPLTFLAGVYGMNFQNMPELGWRYGYYAVWGL
                     MLLIALLLLHLFRKKRWL"
     gene            complement(16438..18072)
                     /locus_tag="PK198_02330"
     CDS             complement(16438..18072)
                     /locus_tag="PK198_02330"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF37599.1"
                     /translation="MTKKLLFAALPAFLLSVSVLRAQVNFQSSNLPIVVINTMGQPIP
                     DEPKIMANMGIIYNGPGQRNFVNNPFNAYAGAIGIELRGSTSQDLSDKKPYAVETRNA
                     DGSNLNVALLGMPSENDWVFLAPFSDKTLIRDVLTFTLARRIMPWAPRSRFVELVLNG
                     NYQGVYAIVERIKQDNNRVNIATLNPDENSGDDLTGGYILKIDKWTGVGNDGFRSNYS
                     PNGSPDREIVYQYHYPAPEDISDPQKNYIRQYINSFENIMASSNYAHPVTGYPAAIDV
                     QSFIDFFLINEITRNVDGYRLSTFLHKNKDSNGGKLKMGPVWDFNIALGNANYCSGGD
                     THGWAADFNSVCGGDFWLIPFWWQRLREDPNFRAQIRTRWQQLRSSTFSNTAITGMID
                     SLTAVVNEGQIRNFQRWPIMGQWIWPNNFVGQNYGSEVNYLKTWLNGRLIWMDGEIFS
                     FPVATAEPSANITAHVFPSPATRGQTIFFECAAAGSSHAVLELFDLAGHRIATQQLDA
                     SSGGFQFRWQSDIPGIYTYQIRIEGRRAAGGKVVVY"
     gene            complement(18150..19214)
                     /locus_tag="PK198_02335"
     CDS             complement(18150..19214)
                     /locus_tag="PK198_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF37600.1"
                     /translation="MQGSFTRFIILLAVACTMALEAAAQDPRFSQFYATPNQINPALN
                     GVFDGTFRFTANYRELYSSILSKDAFRTFAAGVEFRNKVGRSGDYYGLSLSALRDDVG
                     ISNFNRFHAHVGGSYLKQLSGSRYRNSDQYLIAGAQIGAGQRGFDWGRLWFSNQFNSS
                     QAFVDYGSASGEDFQRRETNIFLDFNAGLLWYAIFDKNASVYAGGALHHINEPNISFV
                     ETRDEVLHRKWVGHAGGEIPFSDQLSLLPAVAVMGQHKGMSAALGANVRYTNRDWKEI
                     AVRAGAWAHVVNRLDQGMHLDAIAVTTVLEMERWNLGLSYDVTTSILSAANNSRGAFE
                     MSFIYVQPSQQRFKVSCPRF"
     gene            complement(19254..>21439)
                     /locus_tag="PK198_02340"
     CDS             complement(19254..>21439)
                     /locus_tag="PK198_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37601.1"
                     /translation="STTLTEPALLAIDSTRFTAVRCFGGNDGNASVFVSGGTEPYRFG
                     WDDPAQQLNDTAVFLAARNYAVVVTDANGCMRTANVTVTQPDALQATLTPVHPLCAGE
                     ATGAVQTVVQGGVGNYQYQWSNNQNAATLTAVTEGAYSLTVTDGNGCTVQTQSVLTSP
                     APVTLAVAQSFRGCFGLSDNEATATVGGGVAPYRYSWSNGSQNDRPVDLAPGTYSVTV
                     SDANNCTTTGSANLTDWNLLTANIIAEPPTCNGLADGLMGVNIITGGAGGTASEYIFS
                     WSNNASGAIIRNIPGGLNYIVTVIDRQGCIGTAERFLNQPAPILFDIDHTNALCFGSS
                     DGTATVSNIRGDNVQFSFLWDAAAQTNATASALAAGRYSVTVTDEDGCAASGDVTIGQ
                     PSDVVLRLDLTQNGCFGNTEGTAKLTATGGTPGYTYRWPGNRTGADISGLAAGAYMAT
                     VTDANGCVKNIEAVITQPEPLAAEVSHTDVTCFGGRNGVITVNMSGGTPGFQFSFDNR
                     NFNGANIMVGLRAGQYTIYVKDAKGCTLLTEAAVAQPDEFMVDAGPVSQTIALGDSVV
                     LRATAFNAQGDVEYVWSAAYGNSLSCTECAVTVARPTDQIAYELYAVDANGCESTDLI
                     TIFVEKERIALVPTGFTPNNDGVNDRLLVHGREGTRILFFRVYDRWGQLLYDGGDFLV
                     NDAGFGWDGHFRGSPVEAGVYLWHLEVEYPDGVKERHTGQTTLVR"
     assembly_gap    21440..21486
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21487..>21819)
                     /locus_tag="PK198_02345"
     CDS             complement(<21487..>21819)
                     /locus_tag="PK198_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37602.1"
                     /translation="HQGSNGALTASAQGGNGGYQFAWSNNGTTAAITSLPAGAYEVTV
                     TDQRGCRATAALPIVEPTPVALQLTVTPIACHGVASGAVQALGTGGVGAFTYTWGHTG
                     NGQAQQTSL"
     assembly_gap    21820..21832
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21833..24771)
                     /locus_tag="PK198_02350"
     CDS             complement(<21833..24771)
                     /locus_tag="PK198_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRF37603.1"
                     /translation="MKHMYPFRQFLFIAMFFLFSGMLSAQTFVMSNAPINSCSGFFYD
                     PGGPAGNYGPNQNFTTTICPAGGTGTHVQLVFSAPQMGPGDQLCFFDGPTAAAPQLSC
                     AADFEAGQPFIIQATAVNPGGCLTITFISDGAGEGAGWSADINCVASCQIIQAELVSS
                     DPAVMPVDTGWIDVCPGERVFFTGRGRYPQNGAIYNHSDLTSSFQWTFGDGGSAVGPN
                     VSYVFEEPGGYIVQMIITDQFGCKNTNFLTQRVRVSTQPSFNIGGSLASQICVGDTIT
                     LNAAVDTLDANYVFSVVPMPGSFQSGGIRSDSLPLPDGTGAVFATSIGFTDFSPGQAL
                     TNINDLLGICVNMEHSWLHDLEVSITCPSGQSAILHNFISPPTTGGAGETRLGIPNDP
                     DPAGSPNAINTPWGVIVPGVGWDYCWTPTATRGTWRQFATNNNPGTLPTGDYNSFQPL
                     TNLLGCPLNGEWTLTVIDRWGADNGFIFNWSINFASSLYPRLETFTPQIVDYNWALNP
                     SIIHYSSDSIVAVPTTAGTASYTFMITDNFGCTYDTSINIRVLPFTHPDCYDCIQNVA
                     DLQDTTICSGQSVAFNASPGSLDANVPFAAYPYAPFGFSTHPPANPFNSTINVSSIRP
                     LTLTNPTQQIVSVCIDIETDWCSDLRLFLRAPNGVLLELSTNNGGGGDNYTNTCFTPT
                     AVASIVSGAPPFTGNFRPEGNWSILTNAPINGNWTLVATDAFGFNDIGRINSWTITFR
                     STNTVNYTWTPSTGLSCVNCPNPTATPTATTTYTVQSMDVYNCTYSEMVTVNVVNSIP
                     APMVVCGSQGGGQLTFSWAPVAGYTQYEYREIINGTAGPWQGPVTALTHDVNNLMNAD
                     DVTLEVRVYLPPSAVNCPIAVGSAMCIYETCTLSAVLSGSTQDVSCNGLSDGAATIVA
                     NGGTQPIQFFLDGSATPLGSGIFNTLPAGAHSVLVQDAAQCQVTVNFTINQPPALLAN
                     IVQTQAI"
BASE COUNT         5833 a         6541 c         6594 g         5782 t
ORIGIN      
        1 cgctgttgtc gaactgtgtg acgagcatgt ccgactcttt atcaatcttc accggcacgt
       61 acttggtgat ttcgtcgggg gtgatgacga cgccgcaagc gtggatacct gtgttgcgaa
      121 tggagccttc cagcttttcg gcggtgtgaa tcatctcggc aatttcatct tgcccggcag
      181 cgagttccct gaatttgtaa gccttttcga gttcgtcgcc gttgaggtcc cctttgagtt
      241 tggggtccac gtcgccggga gcgagcactt ttttgagcga tgccttgagg tgcgcgggga
      301 aggttttggc cactctgtcc acctcgctga gcggaatgtt catcacgcgg cccacgtcgc
      361 gcagagagag tttggcggcc atggtgccgt aggtcacgat ttgggctacc tgattgcgtc
      421 cgtatttttc gatgacgtac tggatgacct tgtcgcggcc ttcatcgtcg aagtcaatgt
      481 caatatcggg catggacacg cgctccgggt tgaggaatct ctcgaaaagg aggtcgtatt
      541 tgatggggtc aatattggtg atgccggtac aataagccac cgccgaaccg gctgccgaac
      601 cccggcccgg ccccacggcc acacccattt ggcgggcggt ggtggtgaaa tcctgcacga
      661 tgaggaagta gcccggatag ccggaatttc tgatgacctc cagctcgaaa ttgaggcgtt
      721 cttcgatggc gggcgtgatg gtaccgtagc gtttccgggc gccctcgaag gtgaggtggc
      781 gcaggtattc gtcttgggaa ctgaatccgg caggtaaggg gaatgcaggc aggagcacgt
      841 cgcgggccaa ttggaggttg tccactttgt cgaaaatttc catggtggcg tcaatggaat
      901 gcggcacgtc gtggaagagg cggttcatct cctcctgcgt tttgaagtag aagtcggaac
      961 tggggaagcg gaagcggtct tcctgctcga tgaggctgtt ggtgttcacg cagagcaaca
     1021 cgtcgtgcgg gcgccagtcg tcttcttcga ggcagtggga gtcgttggta caaatgacct
     1081 tgacgttgta tttggtcgcc agccgcagca ggtgctggtt cacatcttcc tgactgatgc
     1141 ccagtccgtc aatgttttcc atgttgcgat ggcgctggat ttcgatgtaa aaatcctcac
     1201 cgaagaggtc gagccaccag cgcaggagcg attcggcttc atccaatttg ccgtgcagaa
     1261 tggcctgcgg aatctctgcg cctatgcaac aactggaagc aatgaggccc tcgtggtatt
     1321 gcaccagcag ttctttgtct atgcgcggat acttgccgta cagcccttcc agaaagccca
     1381 gcgagcagag cttggagagg ttttcgtagc cctgtttgtt tttggccagc aggagttggt
     1441 ggcagcggtt gtcttcttcg cctttggcgc gcgagaatga ttttttgtgg cggtctttga
     1501 ccaagtaaaa ctcgcagccc acgatgggtt tgaggccgcg tttgttggct tctgaaacga
     1561 atttgaaagc gccgaacatg ttgccgtgat cggtgagggc tacgccgcgc tgaccgtcgt
     1621 ttttggcttt gtccatcatg gcggcgatgt cggtggcgcc gtctaagagc gagtattgag
     1681 tatggcaatg aaggtggacg aattccggca tggtattgtt ttttgatcgg tgggtacaaa
     1741 gttatctaaa attacgagga aaagggggcg gctttttacg attcgctcaa attgattctt
     1801 cggatttttc ctgtgccgtc gctctcaaag aggagcttgc cggtttctct tacatatttg
     1861 agagggattt tggggttgtt gatgtctttt cctccgtctt tatgccattt ggcttcccag
     1921 tccgagaagt atggttccag attccccggg gttaatttat aaatttccag aatttcgata
     1981 cctctgtaaa ctgcaaatat ccagtccact tttctgtatt tctcaatgat ggtcggattc
     2041 atgtggtgat gagttgaaaa acttctggta agagatacgt tcaccgattt aagctcgtat
     2101 tccctgcctt ctgcatccac ggcatcgttt ccttcccgcc ccggcaagtt ttttaaccct
     2161 gtgatgagca gtacctgaag caatttgccg ccgttgtctt gaaaaatatc gttgatgccg
     2221 ttttgtgccg ctacatcctg aagcatttgc aaagagggaa atatctggtt aaaaacatca
     2281 ggcgcgtcgc gagcgacttc cgactctttg gcaatatttc cggggatatc ttctccgagt
     2341 aaatcagcga cgtcaatttt taagactttc gctattgnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnaa tgttttgttt tgcattttct atgtaaccga tataggttcg gtcaatatct
     2461 gccctgaaag caaggtcttc ctgagtgagt ccgagttgtt ttctgaactt gactacattg
     2521 cgtgcaatga tttgcaataa atccgccaaa tcgttctgca aggccatttt ttttgcaaaa
     2581 atgatcaatg taaatgggat atttctccga atatattcgg agaaatattt atctttgcca
     2641 catgaaaaaa gcatattcaa cacagtacgg ctctcaaatc atcggtaact cgttggatgt
     2701 tctccagagc tacccggacg aaagtataga cttggtaata accagcccgc cgtttgcgct
     2761 tctcagaaaa aaagagtacg gaaatgaaga tcaggcagaa tacattggtt ggctgtcgca
     2821 gtttgcaaaa ttagtttata acaagctaaa gcccaccggg agttttgtgc ttgatttagg
     2881 gggcgcctac aagagcgggc tgccggtgag gagtctttat aatttcaggg tgccaattga
     2941 gttttgcgac aatatcggat tctatctggc cgaagatttt tattggtaca atccatccaa
     3001 gctccccagc cctattgagt gggtaaataa gcaaaaaata agggtcaagg atgccgtgaa
     3061 caatgtgtgg tggttttcaa aaactccata ccccaaagct gatgttaccc aggtattggc
     3121 gccatacagc gaccgaatga aaaaattgct ggatgatcct gaaagctatt acacacctaa
     3181 agaacggcca tcggggcaca atatcggagc aaatttcatc gacaatggag gggcaatacc
     3241 ttccaacctg ctccaaatac cgaactcgga gtcgaactca agctatcagg caaactgcaa
     3301 aaaagcgggg ttgagtgcgc atcctgcaag gtttccatcc aaattgcctg aattctttat
     3361 caagtatttg accgaaaagg gcgatttcgt ggtagatatt ttcagtgggt ccaacacaac
     3421 gggttttatt gccgaatcac ttaaaagaaa gtggaacagt attgaattgg atgcttctta
     3481 tgcagccaac tcgtcttttc ggttcctgcc taaaactctt tccgaaaaag acttactcaa
     3541 tgtttatttt tcaatcttgg ccggaaaaga aattcctgtg atggattttt taatgaatgg
     3601 ccgcgaagtc tccgagctga ccaaggattt tattcacgcg gaaaagcttc attttgaact
     3661 gagtggaaaa ttgaacaaag cagaaagaag caagcttcag gttattgaaa aggcagccgc
     3721 tgttcttttt tgagtatgcc catatcgctt caccacatac ccatgtttgc tatgaaaaaa
     3781 atcatccttg ttgccctgct catcacatcc gccactgccg cacgggcttt ggaggcgggc
     3841 gtttcttact gcgttttcag gtcggaaacc ggcgcttatg tggagttata tttgtatgtg
     3901 gacggcgcct cggtggagtt tgccgccggc cccgattcca gcctgcaagc cggggtagag
     3961 gtggtgttgt atttcaaaca gcaggatcaa attctgcgtt acgacaagtt tgccctgcac
     4021 agcccccgca tgaagcaacc cgaacacttt atggacatca aacgctatgc cctgcccgac
     4081 ggcgactacg aactgaccgc agaactgaga gatttgaacc gctcgggcaa tgaaaaaacg
     4141 taccagtcgc cgctttccgt tcgcatgtcc ggcaacgaga tattgcaatc gggtattcaa
     4201 ttgctggccg ccgcccgccc cgatacttct caaaatccct tctgcaaaaa cggcatttac
     4261 atggagcctt tggcccggca cacttatggc aaaaccgcct cggaactgat tttttatcac
     4321 gaaatttatc acagcgatct ggctttggcc gatgacttcc tcgtcactta ccgcgttgaa
     4381 acggccacgg cagatgcatc ccggacagtt ctcatcgcgc acaagcggcg ctctcccgcc
     4441 cccgtagtgc cgctcttgtt gccgatggac atcagcagcc tgcctaacgg agactaccgc
     4501 ctcgtggtgg aaatcagaaa ccgcgcccag gaactgaaaa gccgcaggga atctgccttc
     4561 cgccgcgaaa acccctacct gcgcagcgca gccctgcacc ccgacagcgt tcgtatagag
     4621 gatgaatttg tagaaaaact cagcgccgat gaactgcgcc tgagccttcg cgcactcacg
     4681 ccccaattgg ccggcaaaga gcaggaagcg ctccttttgc tcatcaaaag cgacagcctg
     4741 aaggctcaaa aaaactatct gttcagatat tggatacaga aaaatgccaa cgcgcccgaa
     4801 gccgcctatc ggcaatacat gaaagtagta atggctgtag atcagacctt tgcgtcagga
     4861 ttcagacgag ggtttgaaac cgaccggggg catatttatc tcaaatacgg cccgcccgac
     4921 gacatggaaa cccgcgaaca ggaaccctcg gcgccgccct acgaggtgtg gtcttacaat
     4981 caatttccgg ccacaaagca aaacaatgtg cgcttcattt tttacaaccc ttctctggcg
     5041 cccggcgatt ttgtgctgct ccattccgat gccatcggcg aacgccaaaa cccgcaatgg
     5101 ctccgcgacc tataccgcaa tgcccccggc gactgggaca gcgaccccat caacggcaac
     5161 gacatccgcg acggcttcaa ccgaaatgcc aaaagagtgt taagggataa ctgattcggc
     5221 agcttgtggc ttgcagccgc cccaacccgc agcttgcagc taaaggcttg cagccgtccc
     5281 aacctgactt tttaaccacc caattcatac acacctgtat tcatggacat cgaagtacaa
     5341 gaactcaaag ccaaaatgga acgcggcgac tcatttctcc tcttggatgt gagagagcct
     5401 tacgagcgcg aagaattcaa catcggcggc gaatttatcc ccatgggaat gttccccagg
     5461 gctgccgaac atctggaaga ccatcagaac gacgaggtga tcgtttactg ccgctccggc
     5521 cgccgcagcg ccaccgtgca gtacatgctt cagcaagcgg ggttcagcaa tgtgcgcaac
     5581 ctcgaaggcg gcgtactggc ctggatagac gctttcggga ggtagttcgc ccgaccaaag
     5641 ctaacaactc tctgcccggt gctgcaccgt agccggcgca taacccaagt gcgggtcgaa
     5701 accgatgccc ggcgccgcct cccggatgcc gatgcgaatg agcgccaagt gatgaacggc
     5761 atgatcgtgc gcgtacatca actccctgcc caccgaggaa tggaacgcag gtcgttgctc
     5821 gtcgccgtcc tgtgcagaga aatcgcccaa aacagacaag ggcttgcttt catccaaagc
     5881 ctgaatggca tcgctgagtt ctttaaacac ctctccggca gcttcgggca gcgactccat
     5941 gagcgggttg cggtcgcggt gcgtatagtt cacggttccg gcgggaatat cgcgcaggag
     6001 gcattggtaa aaatcgagga tgtgcctgaa gtgttgcccg atgctggagc catgcagcac
     6061 ctccagcgga agtgtgtaag cgggttgcgg caattgatcc agcaggcagc atatttgtgt
     6121 gatgatggcg gcattgccct gtttgctgtt catcggtgaa gatgcttttg agttaaggta
     6181 tgttcagaag tttgtgcgtt tgcaggctca gtctccattg cgggttggcc atacaatatt
     6241 caagcgtcag gcgggtattc gcctcttttt caggcccgtc catcggttgc agatagaaac
     6301 aatcgaaatc ccagtgttcg tacaaaggtg gttctgcgtc cggctgcggg aacacgagtt
     6361 tgagttcgtg gccttttcgg agcaggatat ccgagccggc tttggggctt acgcaaatcc
     6421 aatccaagcc cgccggcgcc tctatagtgc cgttggtttc tacggccacc tcaaaacctt
     6481 gcgcgtgaaa agcggcaatg agcggctcgt ccaattgcag cagcggctcg cccccggtac
     6541 acaccacata gcgtgggccg gaaggcgcag gccagagcga ggctaccttt tgggccaatt
     6601 cagcagccga atagcgcccg ccgttgaggc cgtcggtacc ccaaaaatcg gtatcgcaaa
     6661 actgacaaat ggctttgctg cggtcttctt cccggccgct ccacagattg cagccgctga
     6721 acctgcaaaa caccgccggc cgcccgcttt gtgcgccctc gccttgcagc gtgaagaata
     6781 tttctttgac tttgtaactc atgacaaata agaccgcatg gctgctttag tagtggggca
     6841 aaaataaagt gttcattttc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttctttg
     6901 actttgtaac tcatgacaaa taagaccgca tggctgcttt agtagttttt atcgcccctt
     6961 ggggtgcaaa gtacggcaat tgttaattaa cacagccccc caacatttta ataatgattt
     7021 accgtcttag ccactacact cctgcgagta actatctatt actaaaaaca gacgaccatg
     7081 cgaaaaacac ttttgctgct ccttgtggca gcgctgccct gcattctgac cgcccaaaaa
     7141 acaaccatca gcggctacat cacagacggc tccagcggcg aacccctcat ttcggccaac
     7201 gtatttgaag cccggctcga agtcggcacg gtgagcaacg tgtacggatt tttcagcctc
     7261 acgttagaat cgcgcgacag cgccgtactt ctgttcagct acatggggta tcagccacag
     7321 gaactgcggg tagcgctttc ggccaatgct acgcttcaaa tagcgctgca acccttcgtg
     7381 gaattggcca ccgtagaggt gcgcgccgac cggagcgaac gcatcgagca acgcacccaa
     7441 atgggccgca tagaggtgcc gatagaacaa atcaaacgcg tgcccgccct gctcggcgag
     7501 gtggatgtac tcaaaagtct gcaattgctg cccggcgtac aaagcggcgg cgagggccaa
     7561 aacggcctgt atgtgcgcgg cggaagcccc gatcaaaatc tggtgttgct cgacggcgtg
     7621 ccggtgtata atgtgagtca tgtactcggt attttttctg tattcaatgc cgacgccatc
     7681 aaaaacgtga cgctgaccaa gggcgggttc ccggcccgat atggcggacg actcagcagc
     7741 gtattggaaa tcaacatgaa agaaggcaat accaaagcct ttcacggaga gggttccata
     7801 ggcttgattt cttccaaact cacgctcgaa ggccccatca tcaaagacaa aacctccttt
     7861 ctcatctcgg ctcgccgcac ctacgccgat ctcattttca agcccatcat caaggctgtt
     7921 cagccgcccg atcagaaatt tgacatagcg ctgtattttt atgattttaa tgccaagatt
     7981 cagcacaagt tcaacgaccg ccaccggctc tacctcagcg cttacagcgg agccgatgtg
     8041 tttcaaacaa ccttccggga gcaaaatgac gccttttccg gcggcttgga ctggggcaat
     8101 ctcatttctg ccgcccgctg gaactggcag atttcgcccc gcttgtttgc caataccacc
     8161 ctcacctaca gccgctataa aatagacatc ctttccgcgc aggaaagcaa attcaacaat
     8221 gtggtggaaa aattctcggc caaatacatt tccggcattg aagacctcgg cggtaaaatt
     8281 gatttcgact tcatccccaa tccccgccac tacatccgtt tcggcggcgg cgctaccaat
     8341 cacacctaca accccggcgc cctggccctg aaggaacaat cgggtggaac gacgaatata
     8401 gacacgctgt tggggtcgcg caaggctttc agcactgagt attttctcta tgccgaggat
     8461 gacttctcgc tcggcgcgct caaaatcaac gccggcctgc acgcctccgc ttttgatgtg
     8521 gagggcaaac tttacaccag tctccagccg cgcctcggcc tgcgctacct gatgaagaac
     8581 aaactttcgc tcaaagccgg cttcagcacc atggttcagt acatcaacct cctcaccagc
     8641 gaggcgcttt cattgcccac cgacctctgg gtgcccagta cccggagtgt attgccgcag
     8701 caagcctggc aggtttcggt aggggcggcg cgtacttttt gggatgagtt tgagttcagc
     8761 ctggaagggt attacaaaaa aatgagcaac gtcatttctt tcaaggaagg cgcatccttt
     8821 ctctttggcc tcgaaaacga ctggcaggat aaagtgacgc agggcgaagg cgagagctac
     8881 ggcctggaat tgtttttgca aaaaaaagcc ggcaaaacca ccggctggat aggctatacc
     8941 ctgagctgga actggcggca gtttgacgaa atcaacggcg ggcggcgcta ccccttccgc
     9001 tatgaccgac gccacgatgt ttcggtagta atcaaccacg acttcagcaa gcgggtgggc
     9061 ctctcggcag cctgggttta cggcaccggc aatgctgtga cgctcaatac tttccgatac
     9121 ccaggttata tagggcagtt caccgacatt ttcggaaata ccaccgatta tgtcagcgac
     9181 gtggaatcgg ggggcgaacg caatgccttt cgcatgagag attatcatcg cttggacatc
     9241 agcttagaac tgcgcaaaca gcgccccaaa tcggagcgca aatgggtatt tggagtgtat
     9301 aacgcctact ggcgacgcaa tccgtacttt atgacaactg gatctgtgtt cgaaaacggc
     9361 gtcagccggc gcgtattccg cgaggtcagt attttgccca tcatcccgtc ggtgagttat
     9421 cagttcaagt tttaattcca aaaaaatcca accatgaaat acactgcctt tattttcgcc
     9481 ctgttgtcgg ccatcactgc cctcacatat agttcttgta cagaggattc gttctcccag
     9541 atcgtagaga tagaccttcc cgaacatcgg cccaggccgg ttattgcctc tcaaatcaac
     9601 gccggggaca cgctcgtgca ggccctcgtc agcatcagca tgggtgtttt ggatacgggc
     9661 aattatgttt atccggccaa cgcaaccgtt cgcctgctgc gcgagggtca ggagccgctg
     9721 ccgctcacgt tcaacccaga caaaaaaagc tacacggcgc ccgcacctgc tgcgggtttc
     9781 gtgcctggcg cccgctaccg gatagaagcg caagtgccag gctatggtac cgcttattcc
     9841 gagcagataa tgcccggcgc gcctgatgtg gcaagtgtgg agttgaggag gaatgccgtg
     9901 atcaatccca atggcaaccg ctctgatgaa atgctcatca ccatagacga cccggccggc
     9961 agcgccaatt actatcgcat tgaactgtgg caagagggaa ccctcatttt cctcgacgat
    10021 acgattgagt ataacaaccc ggcgtactta gagagcaacg accccaactt gcaggacggc
    10081 agttccgggg ggaaggttct ggcagacggc gccttcgacg gtacgcgcta catcgttcgt
    10141 ttttcaacag gacgcacttg gatagacgcc tcgaacaccg tccataaata taccctccgc
    10201 attgcccaaa tcacccgcga cgcttatctg tacgaccgca cgatcactca atactaccgt
    10261 gcgctgggca atcctttcgc agagccagtc acggtgcatt ccaatataga gggcggctat
    10321 ggagtatttc gggcggctca ttggagggag tgggagttga ggtagcagat ggtcttccgg
    10381 ctgcattttt tactttttca aaaccacaaa aaactttaaa aaattaggga agcccgcttt
    10441 tatgcccgaa ctttgccgat cctaaccttc ctcgaccggt acaagacaaa tactacaaac
    10501 caacgattat gatcaatctc attcttttcg gccctccggg ttcgggcaaa ggcacacagg
    10561 cgttgaaact cactgaacgc tacggactgc tccacatctc caccggcgac ttgtttcgct
    10621 atgaactggg caacaatacc ccgctgggcg ttctggccag aagctacatg gaaaaaggcc
    10681 agttggtacc cgatgaggta acggtgggca tgctgcgcaa caaagtagaa gccaaccccg
    10741 acgtaaacgg ctacattttc gacggctttc cgcgcaccat cccgcaggcc gaagccttgg
    10801 atgcactgct gaccgaacgc aacgacagca tcaccaaact catcatgctg gatgtgaccg
    10861 aagacgaaat cgtgaaccgc atcctcgaac gcggcaaaaa ttcgggccga gccgacgaca
    10921 acgatccggc catcattcgc aaccgattcg aggtgtatct gaaagaaacc tcgccggtgt
    10981 acgatttcta cgcacaatcg ggcaagtcgg tgaaagtgca tggcatcggc ggcattgagg
    11041 agatttttga aaaactctgc gccgaaattg acgccgtagc atgagtactg cggcccctgc
    11101 aacagcagcg caggaaccct ttttgctgaa aaatgtcttc aaccgcgaac tgttggaggg
    11161 ctatgcgctt gtgttgtcct cggtatggcc ggagttcgat acacatcgtt ttttgaaccg
    11221 ccttttcgac gaggcctggc ctgcgttgga actgaaagag cgcatgcgcc gcatcagcgt
    11281 ggtacttcgg gaatttttgc ccccggacta cgccgaagcg gtgccgatat tggtagcaac
    11341 cacccgccgc atggtggccg aatcgggcga aaaactcacg tttgagtaca tgttcattcc
    11401 cgattttgtg caacatttcg ggcaggatca ttacgaagtt tccgttccgg cattgggcga
    11461 gatcacccgc tggacgagtg cggagtttgc cgttcggccg tttttgctgc gctaccccga
    11521 caggatggcc gcgcaaatgt tggtctggtc gcgccacgaa agtccgatgc tgcgccgcct
    11581 gtcgagcgaa ggcattcggc cccgactgcc ctggggtatg ggtgtgccgg cactcaaaaa
    11641 agaccccgcg cccatactgc ccgtactgga aaatctgaaa aacgaccccg ccgaaaccgt
    11701 gcgccgttcg gtggccaaca acctcaacga catcgccaaa gaccatcccg acctcgtact
    11761 cggcattgcc cgccgttggg ccggtcattc gcccgaaacc gactgggtag tgcgccatgc
    11821 ctgtcggggg ctgctcaaaa aagggcatcc ggaggcgctg gcgctgtttg ggtttgagca
    11881 gggggtagcg ggcatcagcg tggaggcatt gcgcgcagac gcggaagtgc gcatcggcgg
    11941 gcggctggct tttgcttttg agctggccaa tacgacctca caaccgtgcg cgctgcgttt
    12001 ggaatatgcc atcaactact gtacgctcag cggcaaaaca tcgcgcaaga tttttaaaat
    12061 caaagagttg gaaatgaatt ccggcgaacg ggcggcattt gcccgcgcac agcgttttca
    12121 ggatttcacc acgcgcaagc actaccccgg tacgcaccag atagacattc tggtgaacgg
    12181 caaagtgtcg gcatcggcag cgtttgaagt gattagtttt ctacacggat agaaaaaaat
    12241 atggagcata aagcagcctt aaaagacatt gccaaacttt ttctaaagct gggtattatt
    12301 ggctttggag ggcctgctgc gcatattgca atgatgcaaa acgaagtggt tgtaaaaaga
    12361 aaatggctga cagagcaaca tttcctggac ttaataggcg caacaaattt aattcccggc
    12421 cccaacagca ccgaaatggc cattcatatc ggatatgaaa aaggcggctg gaaagggttg
    12481 ataactgccg gtctttgttt catcctacct gctgttttta tcaccggatt tttcgcgtat
    12541 ctctacaaat tatacggtca actgccggaa gtacaaccct ttgtttatgg cataaaacct
    12601 gccattattg ccattatact tggcgccatt tttcctttgg caaaaaaatc ttttaaatcg
    12661 gctgaactca ttttcatcgg gcttcttgtt ttggcagggt cattgctcgg tatcaacgaa
    12721 atatatttga tgttcggcgc gggttttttc gcattgcttt tggcttatgt tcggagcaaa
    12781 aaaagcaatc atatcaatgg tttattgccg ttcactttat tacaaatcac caacacgacc
    12841 ctactctcgg catccaatgc aaaccttttt tggattttcc tgaaaatagg cgccatactt
    12901 tacggaagcg gctatgtttt gttcgctttt ctcgataccg aattagtcgc tacggggctt
    12961 ttaacccggc aacaattgat ggatgccatt gcagtcgggc aatttacgcc cggccctgtt
    13021 ttttcttccg taacttttat cgggtatcaa atcaatggtt ggacgggcgc cgttatttca
    13081 accatcgcca tatttttgcc atcatttgtt tttgtggcat tgctgaatcc tctcgtaaaa
    13141 agaatgcgaa actcagaact cttttctgca tttcttgacg ctgtaaatgc agcctccgtt
    13201 gccctgattg cggccgtttg ttttgaaatg ggcaaagatg ccgttaccga ctggcgaacg
    13261 attttaattg caatggcaag cctctccgtc gcttttggat acagaaaagt aaacagtgtt
    13321 tttgtggttt tgggaggttc attcgttggt tatctgttgg cgctgatatg aatccttttt
    13381 ctttgtaaaa ccgaacacgc cctgcgtggt aatggcgatg gaacatgctg aataaatacc
    13441 gtattctttt actgatcggg agcgtcctcc ttcccttggc ctccggcttt gcgcaaggct
    13501 cttcgctgcc tttggggagc gagtcttacc atattattga ccgactgagc attaaaacag
    13561 gactttacgc gccttttcat accaatgccc ggccacatcc ccgcggcgct gtggcccggt
    13621 tggccatgca ggccgatacg gcagatgtgg aactgagcga cctgtatcgc aaagatttgg
    13681 aatacatttt cagagacaac aacgagtggc tcggcatggc ctcctttgcg actaccctcg
    13741 gcgggcgaaa ggagcgcgcc tggcccgata ccgctctgac gcaagtggaa tgcagcctgc
    13801 tggatgcccg gtatgcccgc tgccgaaagc cgattctggg cttcgcctat cccacgcccg
    13861 ccaatttcat cgaagtcaac gacaaatact ttcacctgcg cgccaacccc atgctcaacc
    13921 tgcactacgc gccgctgagc aatgaggagc aaccggtttt tatcaatcag cgcggcctgg
    13981 aactcagggc cggtgtggac gaccgcattt atttttatgc caatattgtg gagtcgcagg
    14041 cagccctgcc gcaatatgtg gtgcaacgcg tgaggcgaga cattgcactg cccggcgcgg
    14101 cgctttataa accttacaac agcaatgttt tcaacatcca aaacgggtac gattttctca
    14161 atgggcaggg cgtgctgggc tttaatatca gtcggcatgt gggcgctcaa ttgggctacg
    14221 ggaggcattt catcggcaac ggctaccgtt cgatgttgct ctccgatttt gcacaaaact
    14281 accctttcct gcgcctcaac tggaacgtct ggaaatttca ttaccaaaat ctgtatgccg
    14341 agttggacat ccgctcggcc aaccaaaccc gcgacggcac gctcgtttcc aaaaaataca
    14401 tggaggccca ccacctcagc atcaatctgg gcaaaaacgt gaacatcgga gtatttgaaa
    14461 ccgtcatttt tgcgcggccc aatcactttg cgctgcatta cctcaatccg gtgatcctgt
    14521 accgcgcagt ggagcaggcc actggcagcc ccgacaatgt gctcataggt ctggatggcc
    14581 gctggaacat agcgcgccga ttccaactgt atgggcagtt cattttcgac gagtttgttt
    14641 tccgggaatt ggtgctggaa aacaaaggct ggtgggccaa taaatacggc attcaggcgg
    14701 gcctcaaata tgtggatgtg ctgggcattg accatttaga tgtgcaggca gagttgaatt
    14761 tttcgcggcc gtacaactac acccaccgcg actctaccgg cagctatgcg caccacaatc
    14821 aggcactggc tcacccgctg gggggcaatt tcaaggaggc ggtttttata gcccggtacc
    14881 gcccgatgcc caggcttttt gtggaggccc ggctcatcag ggccaatatc ggagaggatg
    14941 cgcccggcca aaactggggc aacaacatcc tgctcaacca cgatacccgg gtgcaggaat
    15001 atggcaacaa tacggggcag ggcattgctg ccgatgtgac gctggccggc cttgacatca
    15061 gttttatggc ggcgcacaat gtttttttcg atgtgcagta tttttatcgc aacaaaacga
    15121 gcgaggaccc gctgcgcaac aaccaggtgc aatacatcgg cggcggtatc cgggtgaaca
    15181 tggcgaagct gcgcatggat ttttaagggc taaggcagcc atttagcaac gtcctttaag
    15241 cggcgagtgg caggccaaca gccggtatct gagttcagcc ttacagccag cgcttttttc
    15301 tgaacaaatg caacaatagc agcgctatca gcagcatcag accccacacg gcatagtacc
    15361 catagcgcca gcccaattcg ggcatgtttt ggaaattcat gccatatacc ccggccaaaa
    15421 aagtgagcgg aataaatatg gtggaaatga tggtgagcac ctgcatcaca ttgttcatac
    15481 gaaaactgag ctgagagagg tagaggtcga aaatgccgtt gagtacatcc cgccaggtat
    15541 caatggcgtc cataatttgg atggtgtggt cgtagatatc gcggataaaa aggcgggtac
    15601 tgtcttgcac cattgggttg tcggagcggg agaaagcatt gacggcctcg cgcaagggaa
    15661 aaacggtgtg tcgcagggcc aacacctgca atttgaggtg gtgaatgctg cgtttggtgg
    15721 actcatcagg ggcggtcagt acttgatgct cctgattatc aatcagttcc tccaatttat
    15781 ccaaaaccaa gtagtagtgg tccacgatgg tatccaacag ggcataaacc aaatagtccg
    15841 cgcctcgttt gcgtattctg ccgtttccat gtaggatgcg atcccgcacg gatgcaaagg
    15901 tgtcatcagg tttttcctga aaagaaagga caaaatcttt tccggcataa atgcctattt
    15961 gttcgtgctg cacctccagc gttgcttcat caaaggtgag ggccggcgca atgataaaaa
    16021 cagcgtcttc ataatcctca aacttggcgc gttgctgtat atcaagcaca tcctccaggg
    16081 ccagcgtatg cactccgaac tgccgtccca actgctcgat gagcggcaca tggtgcagac
    16141 cccgcacatc gtaccatacg acgcctgcca tttcgcgcgc cggcggagcc atttcgcgcg
    16201 ccgactgcat aaacgtatcg gattcagtgt attgcaccaa cacgatgtcg ggcacatcgg
    16261 tatggcggtc gccggtgaat accagcgtgc cgggcggtaa gccgtgtttg cgctgcgttt
    16321 tttttctgcg tctggacatt tgcgtcttta gtaagaatac tgctcgaaag taaaccaggc
    16381 catatcgcct gagattcgga tgttttcgtt ttcacgctcc tctaccgagc cttgcattca
    16441 gtacaccacc accttcccgc cggctgcccg tcgcccctct atccttatct gataggtgta
    16501 aatgcctgga atatctgatt gccagcgaaa ctgaaaaccc ccggaagaag catcgagctg
    16561 ctgtgtggcg atgcgatgcc ctgcgaggtc gaaaagctcc agtaccgcat ggctgctgcc
    16621 ggctgcggca cattcaaaaa agatggtttg gccgcgtgtg gccggactgg gaaaaacgtg
    16681 tgcagtgata ttggcgctcg gttcggcagt tgcaaccggg aaagagaaga tttcgccgtc
    16741 catccatatc aggcggccat tgagccaggt tttgagatag ttgacttcag agccgtagtt
    16801 ctgtcccaca aaattattgg gccatatcca ttgccccata atgggccagc gctgaaaatt
    16861 gcggatctgt ccttcattta ctacggccgt cagggagtct atcatgcccg taatggccgt
    16921 attgctgaac gtagaggacc gaagttgttg ccagcgcgtt ctgatctgcg cccggaaatt
    16981 ggggtcttcc cggaggcgtt gccaccaaaa gggaatgagc cagaagtcgc cgccacaaac
    17041 cgagttgaaa tcggctgccc agccgtgcgt atctccgccg gagcagtagt tggcattgcc
    17101 cagcgcaatg ttgaaatccc aaaccggccc cattttgagc ttgccgccgt tgctgtcttt
    17161 atttttgtgc aaaaacgtgc tgagccggta gccgtccaca ttgcgggtaa tctcgttgat
    17221 gaggaaaaag tcaatgaaag actgcacatc aatggctgcg gggtagccgg tcacaggatg
    17281 ggcgtagttg ctggaggcca tgatattttc aaagctgttg atatactgcc ggatgtagtt
    17341 tttttgcggg tcggaaatat cttccggcgc aggataatga tattggtaaa cgatttcgcg
    17401 gtcggggctg ccgttgggcg aatagttcga ccggaacccg tcattgccga cgcctgtcca
    17461 tttgtcaatt ttcagaatgt aaccgccggt gaggtcgtcg ccggagtttt cgtcggggtt
    17521 gagcgtagca atattgacgc ggttgttgtc ttgcttgatg cgctccacga tggcatacac
    17581 gccctgataa ttgccattga gcaccagctc cacaaagcgg gagcgaggcg cccagggcat
    17641 gatgcggcgg gctaaggtga aggtgagtac gtcgcggatg agggttttat cgctgaaagg
    17701 cgccaagaaa acccagtcgt tttcagaagg catgccgagc aaggctacat tgagattgga
    17761 accatcagca ttccgggttt cgacggcata aggtttttta tctgaaaggt cctgcgaagt
    17821 agaaccccgc agttcaatgc cgatagcgcc ggcataagca ttgaatgggt tattgacaaa
    17881 gttccgctgg ccggggccat tgtaaataat gcccatgttg gccatgattt tcggctcgtc
    17941 gggtatcggc tgccccatcg tattgatgac tacaatgggg aggttggaac tctgaaaatt
    18001 gacttgcgct ctcagtacag atacagaaag caggaacgcc ggcagcgccg cgaacagcag
    18061 tttttttgtc ataaggcaaa tttgaaacag catcgtgaag gggcaactca cacccataga
    18121 aaatcgcagt cgccccttca ctaaaaaaat cagaaccgcg ggcagctcac cttgaagcgc
    18181 tgctgcgagg gttgcacata aatgaacgac atctcgaatg cgccacggga gttgttggct
    18241 gccgacagta tggaagtagt aacatcataa gaaaggccga ggttccaacg ctccatttcc
    18301 aaaacggtcg tcacggcaat agcatccagg tgcatgccct gatccaaacg attgaccaca
    18361 tgcgcccagg cgcctgcccg aacggcaatc tccttccaat cccgattggt ataacgcaca
    18421 ttggcgccca gcgcagcgct catgcctttg tgctggccca ttacggctac cgcgggcagc
    18481 aggctcaact gatcggaaaa aggaatttcg ccgccggcat ggcccaccca tttccggtgc
    18541 agcacctcgt ctctggtttc cacaaaagaa atgttcggct cgttgatgtg gtgcaaagcg
    18601 ccgccagcat aaacggaagc atttttgtca aaaatggcat accagagcaa gccggcatta
    18661 aagtcgagga aaatattggt ttcgcggcgc tggaagtctt ctccgctggc gctgccgtag
    18721 tcaacaaatg cctgactcga attgaattga ttggaaaacc aaagacggcc ccagtcgaat
    18781 ccgcgctgcc cggcgcctat ttgcgctccg gcaatgaggt attggtcgct gttgcggtag
    18841 cggctgccac tcagttgctt gaggtaagaa cctccaacgt gcgcgtgaaa gcggttgaag
    18901 ttggaaatac ccacgtcgtc gcgcagggcc gacaaactca ggccgtaata atcgccgctg
    18961 cggcccacct tattccgaaa ctccaccccg gcagcgaagg tacgaaaagc atctttggaa
    19021 agaatgctgg agtacaactc ccggtaattg gcggtaaagc gaaatgtgcc gtcaaaaacg
    19081 ccgttgagcg ccggattgat ctggttgggc gtggcgtaaa actgtgaaaa acgcgggtct
    19141 tgcgccgctg cctccagcgc catcgtacat gcgacggcca acaatatgat gaaacgggta
    19201 aagctacctt gcataattta ctgattttga aaatgaaaaa ttgaatgccc tgattatctg
    19261 accaacgtcg tttgcccggt atggcgctct ttcacaccgt cgggatactc tacttcgagg
    19321 tgccagagat atacgccggc ttctacggga ctgcctctga aatggccgtc ccagccaaat
    19381 cctgcgtcgt tcactaagaa gtcgccgcca tcatacagca attgccccca gcggtcatac
    19441 accctgaaaa acagaatgcg cgttccttcg cggccatgca ccaacaagcg gtcgttaacg
    19501 ccgtcgttgt tgggagtaaa gcctgtaggc accaaagcga tgcgttcttt ctctacaaaa
    19561 atggtgatca aatccgttga ttcgcagcca tttgcatcaa cagcatacaa ctcgtaagcg
    19621 atctgatcag tggggcgcgc cacggtgacg gcacactccg tacagctcag gctattgcca
    19681 taagccgccg accacacata ttccacatcg ccctgcgcat tgaaggccgt agccctcagt
    19741 accacactgt cgcccagggc tatggtttga gaaacggggc cggcatccac cataaattcg
    19801 tcgggctgtg ccacggcggc ttccgtgagc aaggtgcagc ctttggcatc ttttacataa
    19861 atggtgtact gcccggcgcg caaacctacc atgatgttgg cgccgttgaa attccgattg
    19921 tcgaaactaa actgaaaacc gggtgtgccg ccgctcatgt tcacggtgat gacgccgttg
    19981 cgaccgccga aacaagtcac atcggtatgg ctcacctcgg cagccagcgg ctcgggctgc
    20041 gtgatgacgg cttcaatgtt tttaacacag ccgtttgcgt ctgtaacggt agccatataa
    20101 gcgcctgcag ccagaccgga gatatcggcg ccggttctgt tgccgggcca gcgataggta
    20161 taacccggcg ttcctccggt tgccgtcagt ttggccgtgc cttccgtatt tccaaaacac
    20221 ccgttttgag tcagatcaag ccggagcact acatccgaag gctggccaat ggtcacatct
    20281 cctgaagccg cgcagccatc ctcgtcggtc acggtcactg aatagcgccc tgccgccagt
    20341 gctgatgcgg tggcattggt ttgagcggcg gcgtcccaga gaaacgagaa ttgcacgttg
    20401 tctccgcgaa tattggaaac cgtggctgtt ccgtcgctgc tgccgaaaca gagcgcattg
    20461 gtgtggtcaa tgtcgaacag gatgggcgcc ggctgattca ggaatcgctc tgccgtgccg
    20521 atgcaaccct ggcggtctat tacagttacg atgtagttga ggccaccggg aatattgcga
    20581 ataatggccc cggatgcgtt gttgctccaa ctgaagatgt actcagaggc tgttcctccc
    20641 gcgccgcccg ttatgatgtt gacgcccatg agaccatccg ccaggccgtt gcaggtaggc
    20701 ggttccgcaa tgatgttggc cgttaagagg ttccaatcag tcaggttggc gctgcccgtc
    20761 gtggtacaat tgttggcatc cgaaacagtt acggaataag tacccggagc caagtcaacc
    20821 ggtctgtcat tttgcgagcc attactccat gaatatctgt aaggcgcaac gccgccgcct
    20881 acggttgctg tggcctcatt gtccgacagg ccgaaacagc ccctgaagga ttgggcaact
    20941 gccagtgtca ctggcgccgg agaggtcagt accgattgtg tctgtaccgt gcaaccattt
    21001 ccatcggtga cggtgaggct gtacgcgccc tctgtgactg ccgtcaatgt ggctgcgttt
    21061 tggttgttgc tccattggta ttggtagttg cctacgccac cctgcacgac cgtttgcacg
    21121 gctccggtag cctctccggc acacaaagga tgcaccggag taagcgttgc ctgcaaagca
    21181 tcgggctgtg tgaccgtcac attggcggtg cgcatacatc cgttggcgtc ggtcaccacc
    21241 actgcgtaat ttcttgctgc gagaaacacc gccgtatcgt tcagttgctg tgccgggtcg
    21301 tcccatccaa aacgataagg ctctgtaccg cccgagacaa atacggaggc attgccgtca
    21361 ttgccgccaa agcagcgcac tgccgtaaaa cgagtagaat ctatggccaa cagcgccggc
    21421 tccgtcaggg ttgtggaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnaaga gacgtttgct gcgcctggcc gttgccggta tgcccccagg tgtatgtaaa
    21541 tgcgcccacg ccgccggttc ccaaagcctg aacagcgccg gacgctacgc cgtgacaggc
    21601 aatcggagtt accgtcaatt gtaatgccac cggagtcggt tccacgatgg gcaaagcagc
    21661 cgtagcccgg catccgcgct gatcggtcac cgtcacctca taagcgccgg ccggcaagga
    21721 agtaatagct gccgttgtac cgttgttgct ccaggcaaac tgatagccgc cgttgccgcc
    21781 ctgtgccgaa gccgtgagcg cgccattgct gccttgatgn nnnnnnnnnn nnctgatggc
    21841 ttgcgtttgt acgatattgg ctaataatgc cggaggttgg ttgatggtga aattcaccgt
    21901 cacctggcat tgagcggcat cttggaccaa tacagaatga gcgcctgccg gcaaggtgtt
    21961 gaatatgcct gaaccgagcg gcgttgcgct tccgtccaag aaaaactgga tgggctgtgt
    22021 accgccgttg gctacaatgg tggctgcgcc gtcgctcagt ccgttgcaag acacatcctg
    22081 tgtagaaccg ctcaaaacgg cgctgagggt acatgtttca taaatgcaca tggctgaacc
    22141 aactgcaatg gggcagttca cagcggaagg cggcagatat acccgcactt ccagtgtcac
    22201 gtcatcggca ttcatcaaat tgttgacatc gtgggtaagc gccgttaccg ggccttgcca
    22261 ggggccggct gttccgttga tgatttcccg atattcgtac tgtgtataac ctgccacggg
    22321 cgcccatgaa aaggtgagtt ggcctccacc ctggcttccg caaacgacca tcggcgctgg
    22381 aatgctgttg acgacattca ctgtaaccat ttcagaataa gtacaattat acacgtccat
    22441 agattgtacc gtgtaagtag tagtggcagt gggcgtggca gtggggttgg gacaattgac
    22501 gcaagacagg ccggtactcg gcgtccatgt gtaattgact gtattggtgg accggaaagt
    22561 gatggtccag gaattgatgc gaccgatgtc gttgaaacca aaggcatcgg tggccacgag
    22621 tgtccagttg ccgttgatcg gagcattggt aaggatgctc cagttgcctt cgggacggaa
    22681 attgccggtg aacggaggcg ctccggaaac gatcgaagcc acggctgtcg gtgtaaaaca
    22741 ggtattggtg tagttgtctc cgcctccacc gttgttggta ctcaattcca gcagcacccc
    22801 gttgggcgcg cgtagaaata atcgcaagtc ggaacaccaa tcggtttcta tgtcaataca
    22861 aacggagaca atctgttgtg ttggattcgt aagggtcagg ggcctgatac tactgacatt
    22921 gatggtggaa ttgaacgggt tggccggcgg atgagtggaa aagccgaagg gcgcataggg
    22981 ataagctgca aacggcacat tggcatctaa agaaccgggc gatgcattga aggcgacgct
    23041 ttgaccggag caaatcgtgg tgtcctgcag gtcagccaca ttttggatgc agtcataaca
    23101 atccggatgc gtaaagggca ataccctgat attgatagaa gtatcgtaag tgcagccaaa
    23161 gttgtcggta atcataaaag tgtaactggc cgtacctgcc gtagtgggaa cggctacaat
    23221 ggagtccgat gaataatgaa taatggacgg attgagcgcc caattgtaat ctactatttg
    23281 aggtgtaaag gtctctaagc gaggataaag gctggatgca aaattaatac tccaattaaa
    23341 tatgaagccg ttgtctgccc cccagcggtc tattacggtc agcgtccatt ctccattgag
    23401 cggacagccc agcaagttgg tcagtggttg gaaggagttg tagtctcctg taggaagcgt
    23461 accggggttg ttattggtgg caaattgcct ccaggtacct ctcgtcgctg tgggggtcca
    23521 gcagtagtcc caccccacgc cgggtacaat aacgccccag ggtgtgttga tggcattggg
    23581 gctgccggca ggatcggggt cgttgggaat gcccagccgc gtttcgcccg cgccgccagt
    23641 agtgggcggc gaaataaaat tgtgaagtat ggccgactga ccggaagggc aggtaatgct
    23701 cacttcgagg tcgtgcagcc aggagtgctc catgtttaca caaattccga gcaaatcatt
    23761 gatgttggtc agcgcctgcc ccggcgagaa gtcggtaaat ccaatggagg tggcaaatac
    23821 agcgcctgtt ccgtcgggca agggcagcga gtcggatcga atgcctcccg actgaaaact
    23881 gccgggcata ggcaccacgg agaatacata gttggcatcc agcgtgtcaa cggccgcatt
    23941 gagggtaatg gtgtcgccca cacaaatctg agaggccaaa ctgccgccga tattgaaact
    24001 cggctgggta gaaaccctca cccgctgcgt cagaaaattg gtgtttttgc aaccaaactg
    24061 gtctgtaatg atcatttgaa cgatgtaccc tccgggttcc tcaaacacat aggacacatt
    24121 ggggcctact gccgatccgc catcgccgaa cgtccattga aagctggagg tcaggtcgga
    24181 gtggttgtaa atggctccgt tttgaggata cctcccgcgc cctgtaaaaa acacccgctc
    24241 tccggggcac acgtcaatcc agcctgtatc aacaggcatt accgcagggt cggagctgac
    24301 caactctgcc tgaatgatct gacacgacgc cacgcaattg atgtcggcac tccagccggc
    24361 gccttcgccc gcgccatctg atataaacgt aatggtgaga cagccgcctg ggttgacggc
    24421 cgtggcctga atgataaaag gctgccccgc ctcaaaatcg gccgcacagg aaagctgtgg
    24481 cgccgcggcg gtaggcccgt caaaaaaaca gagctggtcg cccggcccca tctgaggtgc
    24541 agaaaacacc aactgcacat gggtgccggt accacccgcg gggcagatgg tggtggtgaa
    24601 attctgattg gggccatagt tacccgccgg accgcccgga tcgtaaaaaa agccgctaca
    24661 agagttgatc ggcgcattgc tcatcacgaa agtctgagca gagagcattc ctgaaaacaa
    24721 aaagaacatg gcaataaaca aaaactgcct gaacggatac atgtgcttca tggaccgcat
    24781 attttttaaa aatgaatgat tctgtcttgt tgagattata acttgtcgcc tgatgaacac
    24841 ctttgaaaag gaccgccggg aagaccttgc aa
//
LOCUS       DAORLF010000014        24482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1127_length_24482_cov_1.925656, whole genome shotgun sequence.
ACCESSION   DAORLF010000014 DAORLF010000000
VERSION     DAORLF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_24482_cov_1.925656"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK198_02355"
     CDS             <1..284
                     /locus_tag="PK198_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37604.1"
                     /translation="KGLTYDSTLAVVAPDVTPNVSLYYLNVKGWNTRPDENLSNADLE
                     RYALWRATTLVLSDTSYLSRPDMDQWLKHPIGVFDNSIYFYDIRPHWPE"
     gene            complement(295..717)
                     /locus_tag="PK198_02360"
     CDS             complement(295..717)
                     /locus_tag="PK198_02360"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF37605.1"
                     /translation="MAVDKVLIKVRLILYYKGQILLLRQTKPNGGNHTLPGGTVESNE
                     TAPQALVREAHEEAGVVLKEKDLQLVHVLEKVKHGERRLTLYFKTYRWEGRLMAREVH
                     KFKECEWFHLDELPKNLTESVRHVLENYRNGVMFSVIR"
     gene            1044..2117
                     /locus_tag="PK198_02365"
     CDS             1044..2117
                     /locus_tag="PK198_02365"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRF37606.1"
                     /translation="MYYENVLATIGNTPLIKLNKIAQDLPGLILGKVEAFNPGQSAKD
                     RIARYMIEQAEKSGKLKPGATVVEATSGNTGYSIAMACGIKGYKCILTVTSKASAEKI
                     SLLRSMGAEVVVCPVEVAPEDPRSYYSRAEQIARDVPGAFYLAQNFNLDNAGAHYHTT
                     GPELWEQSEGRITHYVCCAGTGGTLSGTARFLKEKNPNIKIIGVDAYGSVLKKYWETG
                     IYDRDEIRPYKVEGLGKNIIPDNMAFDLVDEFIKVTDRASAHRCRQLARLEGLFVGYS
                     SGAAMQAVFQIKDQLKPTDVVAVLFPDHGSRYLGKVYNDEWMKQQGFIGVSHPTSQYD
                     NIKRVYKTYRIKYQRYLRQTLGI"
     assembly_gap    2132..2141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2198..2878
                     /locus_tag="PK198_02370"
     CDS             2198..2878
                     /locus_tag="PK198_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF37607.1"
                     /translation="MKTNKQHQFVSNENVSVRLFNNKFLDSLTRTHVAIPVTMFFLYA
                     IGLIWWTKTATVLTNSQIVVLFFGGTLFFTLIEYIVHRYVYHIAPTTEGRKKFQWTMH
                     GVHHDYPKDKQRLAMPPALSVVVGTVLLGFFRLILGDFGFSFLAGFMVGYALYLLVHY
                     AVHIFRMPSNSFKALWVNHAIHHYSEDEVMFGVSSPLWDYVFGTVPRKEKKREVQVNV
                     EADAEKWL"
     gene            3115..5559
                     /locus_tag="PK198_02375"
     CDS             3115..5559
                     /locus_tag="PK198_02375"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37608.1"
                     /translation="MQLFTPQFWIWPALLLILQSGYAQISGRVLDAQQQPIAFANVLL
                     LHTADSNLISGQISSETGDFEFDTPAPGRYFLKIVMLGYADEVSSPFAIMQNGPAPSP
                     FVFKLQPQATQLTTVELVGRRPLLEQKTDRLVVNVAQSVTYSGGTALDVLQRSPGVRV
                     NRQTQRISLAGKEGVMVMVNGKLSRVPDDALLQMLESISANSIESIEIIHTPPANYEA
                     EGNAGLINIILKEQVKGDYKGINGNYSINGGFGKREKAGMGLLLDLRENNVSAYGRYD
                     AGFDVNPQRFSNYRRVNVAGKTEETDMISHRPGLRTFVQNIRLGADWQLSPKTNLGVL
                     GTFFDRDRRMDAVNDVLFSTNGNLTENMVVNISEVNNYRSYTGNINLQHQFSDTHRLS
                     LDADYVYYNVNRPGQYLNRSYDNNQALINESEMRIALQTPIRAVVYKADWERKLGKGF
                     TWEAGAKWSQMRFDNETLVEQRTPAFPDWLKVDALSSESVFSENIFGAYSSFSFQPVP
                     KTEIKAGLRYEYTATQLGAPGQALVVDRSFGSCFPSLFVSRKFDDTRQINASYSRRIS
                     RPRFSWIAPWLMFNDPTTFQRGNPALLPSITDAWKVSYTMLHFQLGVSYSNESNALRP
                     ISLVDAEKNEQYNTYGNLDHFKSLGLDMSAHFTPEKWWDMQYNLGFFYQTTDFSIEGR
                     GLQLRNTGFTFSGSQSFSLPRKFRIEISGNYDAPHYAGIAFWKATGSLNAGIQKDLGK
                     HWGRLRLNVLDIFQTANWYGLTEQPEVNLYVRTGFGFAERTLMLTWTNDFGNKNLKTA
                     RKRQAGAEEELQRLRQ"
     gene            5602..>5910
                     /locus_tag="PK198_02380"
     CDS             5602..>5910
                     /locus_tag="PK198_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF37609.1"
                     /translation="MPKRFLPGQVFEMRAARTLLDVRTPSEFEQGHIPGALNLPLFSD
                     AERAEVGTLYKQVSPEQAFLKGLEFAGARMRQYVETAMQLAPERRIAVHCWRGGQRSG
                     S"
     assembly_gap    5911..5986
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5987..6651
                     /locus_tag="PK198_02385"
     CDS             <5987..6651
                     /locus_tag="PK198_02385"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-selenouridine synthase"
                     /protein_id="HRF37610.1"
                     /translation="HYALEQLASRPIRMVVLGGKTGSGKTRILHALQQAGEQIIDLEA
                     LAHHKGSAFGAIGEAPQPTFEQFSNDLFEAFDALDAGRPVWVESESKAIGKVQIPNEL
                     WQHICAAPMIEVELPFEQRIRHLMEVYAGFPQEQLAASFERIGKRIGGLNVKLALEAL
                     ADDRYEDAAAVALAYYDKTYRHSLDAMNKGRHFQISPPELNPAAIAGQLIEFLQQEIL
                     WK"
     gene            6642..7685
                     /gene="selD"
                     /locus_tag="PK198_02390"
     CDS             6642..7685
                     /gene="selD"
                     /locus_tag="PK198_02390"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRF37611.1"
                     /translation="MEIKEYKLTQYSHGAGCGCKIAPKVLDQILHHQSEKFHFPQLLV
                     GNEERDDAAVFDLGNGTAVVSTTDFFMPIVDDPRDFGRIASVNAISDVYAMGGTPVMA
                     IAVLGWPINLLPPDVANAVVEGSRQACAEAGIPLAGGHSIDSPEPIFGLAVTGMVAVE
                     HLKKNGGATPGCHLFLTKPLGVGILTTAQKKDKLLPEHATMARDSMVQLNKAGAVFAR
                     LPYVKAMTDVTGFGLLGHLSEMCEASNASALLDWEKIPLLQPEIIHHYLDEGCIPGGT
                     LRNWDSYGHKIGPLSERQRHILCDPQTSGGLLVAVEAAAVEDFLHQAAGQGLQLTAIG
                     EITERGAHLVEIR"
     gene            7707..>7975
                     /locus_tag="PK198_02395"
     CDS             7707..>7975
                     /locus_tag="PK198_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37612.1"
                     /translation="MSQSFSISRLPAQAVSVLFHPLLMPTYMLAVLLLVNPYLFGVNS
                     WADPISKLLLLRVFISTFLLPAVGVGMLRGVGFISSLEMPDKSER"
     assembly_gap    7976..8085
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8086..8406
                     /locus_tag="PK198_02400"
     CDS             <8086..8406
                     /locus_tag="PK198_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37613.1"
                     /translation="GAVIGVFLAFFINIFSKISAHAVGMGGMVAMVVITMFLFSYDTF
                     SLQTAWFGMLHFNMTVVLLAALLAAGLTGTARLWLRAHIPSEVYGGFFVCFLSQLIAL
                     RIMF"
     gene            8429..>8951
                     /locus_tag="PK198_02405"
     CDS             8429..>8951
                     /locus_tag="PK198_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF37614.1"
                     /translation="MKNKHALIVGGGLVGSLWAVFLAKRGYKVDVFERRGDMRTAGYS
                     GGRSINLAMSHRGWRAVEKAGIQEELRQVAIPMQGRMMHSPAGELTFQPYGKEGEAIY
                     SVSRGGLNLELLRIADQHEQVRFHFHQKCVDADVESGKVRFEHALSGEITEAEAPLIF
                     GTDGAFSALRGSIQ"
     assembly_gap    8952..8968
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8969..9799
                     /locus_tag="PK198_02410"
     CDS             <8969..9799
                     /locus_tag="PK198_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRF37615.1"
                     /translation="HRPRFNYSQSYLDYGYKELTIPPGPGGSFLMEKHALHIWPRGNF
                     MLIALPNIDGSFTCTLFMPFEGEYSFEKLQTEAQVSAFFQQFFADAVPLMPDLLHDFL
                     QNPTSALATVRCSPWNVGGRVLLLGDAAHAIVPFFGQGMNAGFEDCTILDGMLDVMGE
                     NWDEVIPLFAQQRVPDANAIADLALQNFIEMRDLVADPEFLLQKRIAALLAERYPQDF
                     MPMYSMVSFSHVPYREALRAGKEQAVLLGRIAALEGIESQLEQHPEVDRLFWEWKRQV
                     "
     gene            9974..>10272
                     /locus_tag="PK198_02415"
     CDS             9974..>10272
                     /locus_tag="PK198_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF37616.1"
                     /translation="MQVAPWRSSALWTAKPFPRCPSGRETLDEAVAAARRAFTSWSKA
                     TIKERVQVFFRFRNLLEKHKSELTELIRLENGKTYGEAEAEVLKGIELSEFACSLP"
     assembly_gap    10273..10355
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10356..>10645
                     /locus_tag="PK198_02420"
     CDS             <10356..>10645
                     /locus_tag="PK198_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF37617.1"
                     /translation="IVASITPFNFPLMVPLWTIPNAISLGNCMILKPSELVPMSAQRI
                     AEMLHEAGLPDGVFNIVNGDREMVEAICDHPDIDAVSFVGSTKVAKIVYRRGT"
     assembly_gap    10646..10729
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10730..11380
                     /locus_tag="PK198_02425"
     CDS             <10730..11380
                     /locus_tag="PK198_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF37618.1"
                     /translation="IAASMSGCAGQRCMAASAMVAVGNTEHIVQRICEESQKIVPGQN
                     LGAVITGAAKERIERYITEAEAAGAKVLVDGRNAVVPGKEGGFYVGPTVIDHVTPDMA
                     IAQEEVFGPVLVIIRTDDIDKAIEIENKSPYGNAASVFTENGGLANYVIERVSAGMTG
                     VNVGVPVPREPFSFGGWNESRFGVGDITGKSSIAFWTKLKKITSKWDPKAWKDWAS"
     gene            11519..11971
                     /gene="tnpA"
                     /locus_tag="PK198_02430"
     CDS             11519..11971
                     /gene="tnpA"
                     /locus_tag="PK198_02430"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRF37619.1"
                     /translation="MGQSLVKNYMHIVFSTKHRKPFILPKFENELYGYIGGICKELDC
                     QPIIVGGHYDHVHILCMLSKKIALMKLVQEIKQSSSKWFKSVDEILKEFYWQDGYGAF
                     SVNYKGVDPVTRYIANQHEHHRNKTFKREFRAFLKMYNIEYDERYVWD"
     gene            12242..>13072
                     /locus_tag="PK198_02435"
     CDS             12242..>13072
                     /locus_tag="PK198_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF37620.1"
                     /translation="MNKTTEITARQKKYLWPNHLLYYTEPLPLERGDGMHVWDVEGNK
                     YLDFFGGILTTCVGHNRPEVVQAVKEQTEKLIHSSTLYPNETHVDLAEKMAEITPGRL
                     QMSYFTPSGTDADETAVFLAQTYTGRQEIVALRHGYSGRSAIGMSLTGQHSWRIGGTH
                     IAGIKHALNPYCYRCPLKMTYPGCGVACAQDIEEVIKTTTSGKIAAFIAEPIQGVGGF
                     ITPPKEYFKMAKEIAEHYGGLFIADEVQTGFGRTGDKWFGIEHWDVEPDIMTMATSIA
                     N"
     assembly_gap    13073..13289
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13290..13546
                     /locus_tag="PK198_02440"
     CDS             <13290..13546
                     /locus_tag="PK198_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF37621.1"
                     /translation="YSVIGDVRGMGLMQGLELVKDRQSKEPSPQATQQLFEATRQLGL
                     LIGKGGLYGNVIRIAPPMTAREDDIATALDLLDKAFARIS"
     gene            13595..>13991
                     /locus_tag="PK198_02445"
     CDS             13595..>13991
                     /locus_tag="PK198_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRF37622.1"
                     /translation="MKNDIVELTEDVSASPLYSEDFAPVPAAARTWNRWHIASIWIGM
                     AVCIPTYMLAAGLIQQGMNWKQALFTILLGNLIVLIPMILNAHVGTKYGIPLPVFMRM
                     NFGVDGAVLAGLLRGLVACGWFGIQTWIGG"
     assembly_gap    13992..14100
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14101..15073
                     /locus_tag="PK198_02450"
     CDS             <14101..15073
                     /locus_tag="PK198_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRF37623.1"
                     /translation="FLLFWGMNIYIIYRGIESLKMLETWAAPFLLLIGIGLLVWAWMR
                     VGSMGDILAASYMLGAESKVSFWKIFWPGLTAMVGFWATLSLNIPDFTRYAKSQKDQI
                     IGQIIGLPGTMVLFSYIGIAVTSATLLIYGEAIWDPVALMARFDSPGTVAISMIGLTI
                     ATLTTNIAANVVAPANSIANIMPDKISFRMGGYITGIIGILIFPWKLLADPQGYIFVW
                     LIAYSALLGSLAGIMICDYFVVHKTRLHLAELYKPDGRYRGWNLRAWVAFTVGLLPVL
                     PGFLATVGALPAQSVSAFWTDLYSYAWFVTFLLSFVVYWLLSKMREK"
     gene            15080..>15317
                     /locus_tag="PK198_02455"
     CDS             15080..>15317
                     /locus_tag="PK198_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF37624.1"
                     /translation="MSILIRNGRIVTASEDYVADIYIEGETVHSIGKNLPMQADQIID
                     AGGMVVMPGGIDPHVHLQMPFMGTWSSDSYETGTL"
     assembly_gap    15318..15335
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15336..16455
                     /gene="hydA"
                     /locus_tag="PK198_02460"
     CDS             <15336..16455
                     /gene="hydA"
                     /locus_tag="PK198_02460"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRF37625.1"
                     /translation="TTMVIDFVLQTQGKSLYHALEAWRGRSDGNCYGDYSFHIAVTDY
                     NNDTRQEIGSLVENEGITSFKTFMAYKGALMIDDGQMVDLMQEVKRHGGMVTVHATNG
                     DMIDKLIAKHRAEGKLSPLYHYLSQPEVTEAEASGRFADMAHYTGVQGYIVHMTCQGA
                     LDAVRRATFRNQKVYAETCIQYLVLDASLYEKDPEGAKWVMSPPLRQKKDQEALWAGI
                     DQGLVQLVATDHCPFTWEQKMMGKDDFSKIPNGHPAIEHRMELLWSEGVAKGRISANK
                     YVELSSANAARIFGMYPKKGCIAPGSDADIVIFDPNERHTLSAKTHHMNVDYSAYEGH
                     EVQGKCKTVILRGKVAVDEGKVLIEKGYGQYVKRKAMK"
     gene            16468..16656
                     /locus_tag="PK198_02465"
     CDS             16468..16656
                     /locus_tag="PK198_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37626.1"
                     /translation="MKQEEKEKLEEEILMLRVLLDFHVQNKSYVTMGERDFEEYVNAV
                     LDRLNELENFLKEENDRL"
     gene            16653..>16838
                     /locus_tag="PK198_02470"
     CDS             16653..>16838
                     /locus_tag="PK198_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37627.1"
                     /translation="MKKYAHIHEVVSRLTDDGALRTFFTLYLMAESTSERVRLNEQFQ
                     VEMSSLTSLEQKLMKAEL"
     assembly_gap    16839..16953
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16973..17839
                     /locus_tag="PK198_02475"
     CDS             16973..17839
                     /locus_tag="PK198_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF37628.1"
                     /translation="MPRIIKSGLIQMSLPLTEGQGSIEEIMEAMVQKHIPYIEEAGRQ
                     GVQILCLQEIFNTPYFCPGQDAAWYASAESVPGPTTERMAEYAKKYGMVIIVPIFEKE
                     QAGVLYNTAAVIDADGTYLGKYRKNHIPHTSGFWEKFFFKPGNLGYPVFETRYAKVGV
                     YICYDRHFPDGARILGLNGAEIVYNPSATVAGLSQYLWRLEQPAHAAANGYFMGCINR
                     VGTEAPWNIGKFYGSSYFVDPRGQIFAQASEDKDELLVAEFDLDMIDQVRSVWQFFRD
                     RRPETYGKLTEL"
     gene            complement(17843..>18095)
                     /locus_tag="PK198_02480"
     CDS             complement(17843..>18095)
                     /locus_tag="PK198_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF37629.1"
                     /translation="IKRKDPPLATIEILSPTQVLQTLIDKTYDYFSFGVKSCWIVLPT
                     LQSIYVFRAPGVHEAFNLGDVLSDPNLGIQIPMNELFAR"
     assembly_gap    18096..18108
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18109..18344)
                     /locus_tag="PK198_02485"
     CDS             complement(<18109..18344)
                     /locus_tag="PK198_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37630.1"
                     /translation="MDTANTSGLSDYEIERGKPLPSFLHAVTETRLTVYLVKHYKELY
                     EAFPELTLDTPGKSMTPDISVYRKEPIDFSQDVI"
     assembly_gap    18488..18582
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18583..19764
                     /locus_tag="PK198_02490"
     CDS             <18583..19764
                     /locus_tag="PK198_02490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF37631.1"
                     /translation="PTGIDIPLFIRQIHTHNAEGAARTIYDSNYFGNICGKVCPTEVL
                     CEGACVYNHQHVKPIEIGRLQSYATSQVVNEQKRLFSPAPANGKKVAVIGAGPAGIAC
                     ACELRLAGCEVEVFEAKEQPSGLVLHGCAPYKIANEDALNEVAWLQSQFGFNIHYNHN
                     IESREQWQALEQQYDAIFLGIGLGATQAAGLRGEDLEHVSGATEYIEQIKLDPMSVRV
                     GRKVVVIGGGNTAMDAASESARLGAEEVTLAYRRPKPEMKAYEFEYELAKSVGVKGLF
                     NVAPVAILGDRQVEGIRLAATRVEAGRLQLVDGSEFDLACDAVILATGQEKRSALVAQ
                     IGGIETDRQGRIVVHPETFQSSHPKYFAGGDAVNGGAEVVNAAAHGKAAARGILGRLN
                     G"
     gene            19789..20313
                     /locus_tag="PK198_02495"
     CDS             19789..20313
                     /locus_tag="PK198_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF37632.1"
                     /translation="MFVHLENVPRFYKNYVKLVEETDILQALRISGERMMEVLASLTE
                     AQQDHRYAEGKWSIRELLCHIIDAERIFAYRALRFARKDQTPLAGWEENDYAPEANAE
                     GRSLSHIIGEMKRLRQSTIDLFEGFSPEMLDRKGIANNNEISVAALGFVIAGHEIHHR
                     NILTERYLTHSATL"
     gene            20326..>21186
                     /gene="preA"
                     /locus_tag="PK198_02500"
     CDS             20326..>21186
                     /gene="preA"
                     /locus_tag="PK198_02500"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HRF37633.1"
                     /translation="MPDLSANLAGIKSPNPFWLASAPPTNSGYQIMKAFDAGWGGAVW
                     KTLGVPVVNVSSRYGAINYRSNRMVGFNNIELISDRPLRDNLREIEEVKKYFPHHAVV
                     ASLMVGSRAEWHQIVKDAENAGADGLELNFGCPHGMCERGMGSAVGQEPEVLKLITSW
                     VMEVATVPVIVKLTPNISDITEPAQAAAQGGANAISLINTIQSLVGVDIDNFVSYPVV
                     DGKSTNGGYCGPAVKPIALNMVKNCAQHPGVRLPISAIGGIENWRDAVEYLLLGASNV
                     QVCTAVMHYGF"
     assembly_gap    21187..21219
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21220..21672
                     /locus_tag="PK198_02505"
     CDS             <21220..21672
                     /locus_tag="PK198_02505"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF37634.1"
                     /translation="MIPGLENYMRDKGFQRLDDFIGKALPNVLHWEDLNLHYDVKASI
                     NPDKCIQCQLCYTACEDGAHQAIRIPEDGSRIPQIIEENCVGCNLCSLVCPVENCITM
                     VRKDDGSRHENWKDFVVAGKAPETFDDERAGGLGHWVPAPTDALKKRG"
     gene            complement(21784..22191)
                     /locus_tag="PK198_02510"
     CDS             complement(21784..22191)
                     /locus_tag="PK198_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRF37635.1"
                     /translation="MNVVIDTNCLIVSIPRKNPEYWLYEAFVAQKFQWVISNEILHEY
                     EEQLAQFYSPLTADIVLKILTTAPNVVFQEPFFRWNLIEADPEDNKFADLAISANVHY
                     LVTHDRHFKVLETIPFPTVSVLKLPEFKIALNW"
     gene            complement(22188..>22382)
                     /locus_tag="PK198_02515"
     CDS             complement(22188..>22382)
                     /locus_tag="PK198_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37636.1"
                     /translation="EAQLTLIRLFSRKMEQEEFQALQKILLSFYDDALQKELDRVIAQ
                     KNIQPEDFETRLELQQRTLP"
     assembly_gap    22383..22392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22393..22666)
                     /locus_tag="PK198_02520"
     CDS             complement(<22393..22666)
                     /locus_tag="PK198_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37637.1"
                     /translation="MKRGKADAVFSRALNFYQPSECFNEMEAELKWGVPAHYHPHGVP
                     SGASVAPPIVVTTFGPLTRSDKYSVNPKQANAVKFIQSTAYATRSAQ"
     gene            22694..>24126
                     /locus_tag="PK198_02525"
     CDS             22694..>24126
                     /locus_tag="PK198_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37638.1"
                     /translation="MQFLTRLILLPLLLASGALMAQQRYTISGEIKDAGNGETLIGAT
                     VTVRGQGTGATTNEYGFYSLTLPAADSVLLEFSYVGYQTELRSVSLQKPLIINVELSS
                     GIQMEEVVVKANSFREKARSVEMSVEEIKAADVKLLPVLLGESDILKALQLKPGIPSG
                     SEGTTGLFVRGGGADQNLILLDEAVVYNANHLFGFFSTFNTDAVKDLKLYKGGFPAQY
                     GGRLSSVIDVKLKEGNSKKWAATGGIGLIASRLTVEGPIEKDKASFMISGRRTYFDLI
                     TRGVNSANKDNANYSPIPDYYFYDLNTKINYDLGERDHLYLSGYFGRDVFGFDGDFFN
                     FNFNWGNATGTARWNHVFNPRLFANTTFTFSDYRYNISNKLTGFSFDLGSSIRDAALK
                     TDFFYAVNPRHTLRFGAALTHHYFQVGRLKAGNRDGSIAFSSGQDFNGLEGALYVADE
                     LDLSEKTKLNSGLRLSGFTNGDRFYANA"
     assembly_gap    24127..24269
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24270..>24482
                     /locus_tag="PK198_02530"
     CDS             <24270..>24482
                     /locus_tag="PK198_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37639.1"
                     /translation="WYPSTDRVPPQRSDQVAVGLSYLLGNQYLITLEGYHKYLHNQLE
                     FVDGARLFANNNLEDEFAIGRGRSNGL"
BASE COUNT         5625 a         6410 c         6163 g         5151 t
ORIGIN      
        1 aaaagggcct tacttacgac tccacgctgg ccgttgtagc ccccgatgtg acgcccaatg
       61 tgagcctgta ctatctgaat gtaaagggct ggaatacccg ccccgacgaa aacctgagca
      121 atgccgatct ggaacgttac gccctttggc gggccactac cttagtgctg agcgacacct
      181 cctacctgag tcgccccgac atggatcaat ggctgaagca tcccatcggc gtgttcgaca
      241 actccatcta tttttatgat atccggccgc attggccgga atgagtgata accctcaccg
      301 gataacagaa aacatgaccc cgtttcggta gttttcgagt acatgccgaa cagactcggt
      361 gaggtttttg ggcagttcgt ccaaatgaaa ccactcgcac tccttgaatt tgtgaacctc
      421 gcgggccatg aggcggcctt cccagcgata ggttttaaaa tacagtgtga ggcgccgctc
      481 gccgtgtttt actttttcca gcacatgcac caattgcagg tctttttctt ttagaacgac
      541 gcctgcctcc tcatgcgcct ccctgacgag cgcctgtggg gcagtttcat tgctctctac
      601 cgtgccgccc ggcagcgtgt gattgcctcc gttgggtttg gtttggcgca acaacagtat
      661 ttgtcctttg tagtaaagga tgaggcgcac tttgatgagt accttgtcaa cagccatgac
      721 taagtcgttt tcagcgtaag ggcaggcggc ctgccgggca aatatacgcg ctgcccgaag
      781 aattggttca atttagggtg gcgatataca cgcaaaaaga atttacctac ctttgccgta
      841 gaaaaaatat ctcgcaccgc aaggtgcgaa gccgcttttg tcataccggg cgctgctaat
      901 caataacttg tgccgaaggc tatgcacaaa acctggcggc gcaaggtata gccactgtct
      961 ttatttgtgc gtcatatctc attgaacgtt gaagcccatt ggtcttttgg tgaataagcc
     1021 gcaaaagcaa aaatggtaac gacatgtact acgaaaacgt cttagcaact atcggcaaca
     1081 cgccgctcat caaactcaac aagatcgccc aagatcttcc aggtctcatt ttaggcaaag
     1141 tagaagcctt taatcccggc cagtcggcca aagaccgcat tgcccgctac atgatagagc
     1201 aggccgaaaa atccggcaag ctcaaacccg gcgccaccgt ggtggaagct acatcgggca
     1261 acaccggcta cagcattgcg atggcctgcg gcatcaaggg ctacaagtgc atcctcacgg
     1321 tgaccagcaa agcctctgcc gaaaaaatct cgctgctccg ttctatgggc gccgaggtag
     1381 tggtgtgccc ggtagaggtg gctcccgaag acccccgctc ttactactcc cgtgccgaac
     1441 aaatagcccg cgatgtgccg ggcgcttttt atctggcgca gaacttcaac ttagacaatg
     1501 ccggcgcgca ctaccatact accggccccg aactttggga gcaatccgaa gggcgcatta
     1561 cgcactacgt ttgttgtgca ggcaccggcg gtaccctctc cggcactgcg cgttttttga
     1621 aagaaaaaaa tccaaacatt aaaatcatcg gggtggacgc ctacggatcg gtgctcaaaa
     1681 agtactggga aacaggtatt tacgaccggg atgaaatccg cccgtacaag gtggaaggtt
     1741 tggggaaaaa catcataccc gacaatatgg ccttcgatct ggtggatgag ttcatcaaag
     1801 tgaccgaccg ggccagcgcg caccgttgcc ggcaattggc tcgtctggaa ggcttgtttg
     1861 taggatattc cagcggggct gccatgcagg cggtgttcca gataaaagac cagttgaaac
     1921 ccaccgatgt agtagccgta ctcttccccg atcacggcag ccgctacctc ggcaaggttt
     1981 acaacgacga gtggatgaaa caacagggct tcataggcgt ttcgcacccc acttcgcaat
     2041 acgacaatat caaacgggtt tacaaaacct accgcatcaa ataccagcgc tacctgcgtc
     2101 agacattagg catttagtcg aaattaacgc cnnnnnnnnn ngaagcagat tagcgagatg
     2161 gctcgtaact ttgcgccctc actaattttt tacaccaatg aaaactaaca aacaacacca
     2221 atttgtatcg aatgaaaatg tttcggtgag gctattcaat aataaatttt tagacagcct
     2281 cacccgcacg catgttgcca ttcctgttac catgtttttc ctgtatgcca tcggcttgat
     2341 ctggtggacc aaaaccgcta ccgtacttac caacagccag atcgttgtgc ttttcttcgg
     2401 cggtacttta ttctttacgc tgatcgaata tatcgtacac aggtatgttt atcacatcgc
     2461 acctacaacg gaaggccgca agaaatttca gtggacgatg cacggcgtac accacgacta
     2521 cccgaaagac aagcagcgat tggccatgcc gccggccttg agcgtagtcg tcggcactgt
     2581 attactgggc ttttttcggc tcattttggg cgatttcggc ttttcattcc tcgccggttt
     2641 tatggtgggc tacgctctgt acctgctggt gcattatgcg gtacacattt ttcgcatgcc
     2701 ttccaattca ttcaaggcac tgtgggtcaa ccacgccatt caccattaca gcgaagacga
     2761 ggtgatgttt ggcgtatctt ctccgctgtg ggactatgtg tttggcaccg tacctcgcaa
     2821 agagaaaaaa cgcgaagtgc aggtaaatgt ggaggccgac gccgaaaaat ggctttgatt
     2881 tttgtcaaaa ctcagataca gacacttgaa aagcaggtgt ttgtatctga atttagttct
     2941 taattccctt ctttctgaaa gcgacaaaac gctgttcccc ttatttatta tatagaaaat
     3001 agcttccctt actttggtaa cattccacga atattgaaag ctctccggca gcttttcgca
     3061 tagcggcgag tggaactgat gttatctttt acacactatt gaataaaaaa ctacatgcaa
     3121 ttatttactc cccaattctg gatttggccg gcgttgctgc tgattctgca aagcggttat
     3181 gcccaaatca gcggccgggt gcttgatgct caacaacaac cgattgcttt tgccaacgtg
     3241 ttgttgcttc atacagccga ctccaatctg ataagtggac agatcagctc cgaaaccggc
     3301 gattttgagt ttgatacacc cgctccgggg cgctactttc tcaaaattgt tatgctggga
     3361 tatgccgatg aggtttcctc gccatttgcg atcatgcaga atggcccggc tccaagtcct
     3421 tttgttttta aactgcaacc tcaggcaact caattgacca ccgtggaatt ggtagggcgc
     3481 aggcccttgc tggagcaaaa aacagaccgc ctggtagtga atgtggcgca atctgtcact
     3541 tactccggag gtacggctct ggatgtacta caacgctcgc cgggtgtgcg cgtcaaccgg
     3601 caaacgcagc gtatatcgct ggcaggcaaa gagggcgtca tggtgatggt gaacggcaaa
     3661 ctgagccgag tacccgacga cgcattgctg caaatgctgg aaagcatcag tgccaacagc
     3721 attgaaagca tcgagatcat tcacaccccg ccggccaatt atgaggccga gggcaatgcc
     3781 ggactgatca acatcatact caaagagcag gtgaaaggcg actacaaagg catcaacggc
     3841 aactattcga tcaacggagg atttggaaaa cgcgaaaaag caggcatggg gctgttgctc
     3901 gatcttaggg aaaacaacgt gagcgcgtat ggccggtatg atgccggctt tgatgtcaat
     3961 ccgcagcgtt tcagcaatta tcgccgcgtc aatgtggccg ggaaaaccga agaaaccgat
     4021 atgataagcc accgccccgg cttgcgcacc ttcgttcaga acatacgcct cggcgccgac
     4081 tggcaactca gccccaaaac caacttgggc gttttgggca cttttttcga ccgcgaccgc
     4141 cgcatggatg ccgtcaacga cgtgctgttt tccaccaatg gaaatttgac ggaaaatatg
     4201 gtcgtcaata tttccgaagt caacaactat cgctcttaca caggcaatat caacctccaa
     4261 caccaattca gcgatacgca ccggctgtct ttggatgcag actatgttta ttacaatgtt
     4321 aaccggccgg ggcaatacct caaccgatct tacgacaaca accaagccct gataaatgaa
     4381 agtgagatgc gcattgcgct gcaaacgccc attcgtgccg tagtgtacaa agccgactgg
     4441 gaacggaaat tgggcaaagg atttacctgg gaggccggcg ccaagtggtc gcagatgcgc
     4501 tttgacaacg aaacattagt agagcagcgt acccccgctt ttcccgattg gctcaaggta
     4561 gatgcgctta gttcggagtc tgttttttct gaaaacatct tcggagccta cagctcattc
     4621 agctttcaac ccgtgcccaa aacagagatc aaagcagggc ttcgctatga atacacggcc
     4681 acccaactgg gcgcccccgg ccaagccctc gtagtagatc gcagtttcgg cagttgtttt
     4741 cccagcctgt ttgtgagccg aaaatttgac gatacccgcc agatcaacgc ctcctatagc
     4801 cgccgcatca gccgcccgcg cttctcctgg attgccccct ggcttatgtt caacgacccc
     4861 accactttcc aacgcggcaa tcccgctctg ctgccttcca tcaccgacgc ctggaaggtt
     4921 tcttatacca tgttgcactt tcaattgggg gtctcgtaca gcaatgagtc caacgcgctg
     4981 cgccccattt cattggtgga tgccgaaaaa aatgagcaat acaacactta cggcaacctc
     5041 gaccatttca aaagtttggg cttggatatg tcggcgcatt ttacccctga aaaatggtgg
     5101 gatatgcagt acaatctggg atttttttat caaaccaccg atttcagcat cgaaggcaga
     5161 gggctgcaat tgcgcaacac cggcttcact ttttcggggt cgcaatcctt cagcctgccg
     5221 cgcaaattcc gcatagagat ttcgggcaat tacgatgccc cacactacgc cggcatcgcc
     5281 ttttggaaag cgaccggttc gctcaatgcc ggtatccaaa aagacctggg caagcattgg
     5341 ggccgcctca ggctcaatgt acttgatatt tttcagacgg ccaattggta cggcctcacc
     5401 gagcagccgg aagtcaactt atacgtcaga accggtttcg gctttgcaga acgcaccctg
     5461 atgctgacct ggaccaatga ttttggaaac aaaaacctca aaacggctcg caaaaggcag
     5521 gcaggcgccg aagaagaact gcaacgcctg cgccagtagt ctgaaaaaag gtacttttgc
     5581 cggcaagttg agtaccgtat tatgcccaaa cgttttttgc ccggacaagt ctttgaaatg
     5641 cgcgctgccc gcacgttgct ggatgtgcgt acaccgtccg aatttgagca gggccacatc
     5701 cccggagcgc tcaatctgcc actgttttct gatgccgaac gcgccgaggt aggcacactt
     5761 tacaaacaag tcagtcccga acaggcattc ctcaaaggtt tggaatttgc cggcgcccgt
     5821 atgaggcaat atgtagaaac cgccatgcaa ttggcccccg aacgccgcat tgccgtacat
     5881 tgctggcggg gcggacaacg cagcggcagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca ttatgccttg
     6001 gaacaattgg cctcgcgccc tattcgcatg gtggtgctgg gcggcaaaac cggctccggc
     6061 aagacccgta ttttgcacgc attgcaacag gccggcgaac aaattatcga tctcgaagcc
     6121 ctggcacacc acaaaggatc ggctttcgga gccattggag aagcgccgca accgaccttt
     6181 gagcagtttt ccaacgacct gttcgaggca tttgacgcat tggacgcggg gcggcccgtt
     6241 tgggtggaaa gcgagagcaa ggccattggc aaggtgcaga ttcccaatga gttgtggcaa
     6301 catatctgtg ctgcgcccat gattgaggtg gaactgccgt tcgagcagcg catccgccac
     6361 ttgatggagg tgtatgccgg gttcccgcag gaacagttgg ccgcctcctt tgagcgtatt
     6421 ggcaaacgca tcggcgggct gaatgtgaag ctggcgctgg aggctttggc cgatgatcgc
     6481 tatgaagacg cggctgccgt cgccttggct tattacgaca aaacctaccg ccactcattg
     6541 gatgccatga acaaaggccg gcattttcag atttcaccac cagagttgaa tcctgctgcc
     6601 attgcggggc aactcatcga atttttacaa caagagattt tatggaaata aaagaataca
     6661 aactcacgca gtatagtcat ggcgcgggtt gcgggtgcaa aattgccccc aaggttttgg
     6721 accagattct tcaccaccag agcgaaaaat tccactttcc gcaattgctc gtgggcaacg
     6781 aagagcgcga cgacgccgcc gtgttcgatc ttggcaatgg aactgcggtg gtaagcacca
     6841 ccgatttctt catgcccatt gtggacgacc cgcgcgattt tggccgcatt gcctccgtca
     6901 acgccatcag cgatgtgtat gctatgggag gcacgcctgt catggccatt gccgtgctag
     6961 gctggcccat caacctgttg ccgcccgatg tggccaacgc cgtggtagag ggcagccgcc
     7021 aagcctgcgc cgaggcgggt attcccttgg ccggcgggca cagcatagac agccccgaac
     7081 cgattttcgg cctcgcagtc acagggatgg tagcggtaga gcatctcaaa aagaacggcg
     7141 gcgccacgcc cggctgccat ctgtttctca ccaaaccatt gggcgtaggc atcctcacca
     7201 ccgctcagaa gaaagacaag ttgctgcctg aacacgccac gatggcccgc gacagcatgg
     7261 tgcaactcaa caaagccggg gccgtttttg cccgcctgcc ttacgtcaaa gccatgaccg
     7321 acgtgaccgg cttcgggctg ctgggccacc tcagcgagat gtgtgaggcc agcaatgcct
     7381 cggcgctgtt ggactgggaa aagattccgc tgttacagcc cgaaatcatt caccactact
     7441 tggacgaggg ctgcattccc ggcggcaccc tgcgcaattg ggacagctac ggccacaaaa
     7501 taggtccgtt gagcgagcgc caacgccata tcctctgtga tcctcaaacc agcggaggcc
     7561 tgctggtagc cgtggaggcc gccgccgttg aagacttcct gcatcaggcc gccgggcagg
     7621 gcttgcagct caccgctatc ggcgagatca cggagcgcgg cgcccacctc gtcgaaatcc
     7681 gataacattg gtatctttgc agcaatatga gtcaaagttt ctccatatcc cgtttgccgg
     7741 cacaggcagt atccgtgctg tttcatcccc tgctgatgcc cacctatatg ctggccgtgt
     7801 tgctgctggt gaatccgtat ctttttggcg tcaatagttg ggccgatccc atcagtaagt
     7861 tattgttgtt gcgcgtattc atttccacct tcctgttgcc ggcggtgggt gtgggtatgc
     7921 tacggggcgt gggtttcatc agttcattgg agatgcccga taaaagcgag cgcatnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgc cgttattggg
     8101 gtctttttgg cattttttat caatattttt tccaaaatca gcgcgcacgc agtagggatg
     8161 ggcggcatgg tggcgatggt ggtcattacc atgtttttgt tcagctatga tacattcagt
     8221 ctgcaaacgg cctggttcgg gatgctgcat ttcaacatga cggtagtgct gctggcggca
     8281 ttgctggctg cgggattgac gggcacggca cgactgtggc tccgagcgca cattccatcg
     8341 gaggtgtatg gcggcttttt tgtgtgcttt ttgtcgcagt tgattgcgtt gaggattatg
     8401 ttttgaaatg aaattcatct gattcattat gaaaaataaa cacgcgctca tcgtcggcgg
     8461 cggattggta ggttcgttgt gggcagtatt tttggccaaa cgcggctaca aagtggacgt
     8521 atttgaacgt cgtggcgaca tgcgaacagc aggctacagc ggcggcaggt ccatcaactt
     8581 agccatgagc catcggggct ggcgcgctgt agaaaaagca ggcatacagg aagaactgcg
     8641 ccaggtggcc attccgatgc agggccgcat gatgcacagc cctgcgggcg aactcacgtt
     8701 tcagccttac ggcaaagagg gcgaggccat ttactcggtt tcgcggggcg ggctgaacct
     8761 ggagctgttg cgcatagccg atcagcatga gcaggtgcgg tttcattttc atcaaaaatg
     8821 tgtggatgcc gatgtagaat cgggcaaagt caggtttgaa cacgcgctca gtggcgagat
     8881 tacagaagcc gaagcgcctc ttatttttgg tactgacggc gcgttttcgg ccttgcgcgg
     8941 cagtattcag cnnnnnnnnn nnnnnnnnca ccgtccgagg ttcaattatt cgcaatcgta
     9001 tttggactac gggtacaaag aactgaccat tccacccggc cccggcggca gcttcctgat
     9061 ggaaaaacac gcgctgcata tctggccccg cggcaatttc atgctcattg cgctgcccaa
     9121 catagacggc agttttacct gcacgctctt catgcctttc gagggggaat attcattcga
     9181 aaagctgcaa acggaagctc aggtatcggc tttctttcaa caattctttg ctgatgccgt
     9241 gccgctgatg cctgatttgc tccatgattt tcttcaaaat cctacctctg cattggccac
     9301 cgtgcgatgc agcccctgga atgtgggcgg tcgggtactg ttgctgggcg acgccgcaca
     9361 tgccattgtg ccgtttttcg ggcagggtat gaacgcaggc tttgaagact gtaccatttt
     9421 ggacgggatg ttggatgtga tgggagaaaa ctgggatgaa gtgattccgc tttttgcgca
     9481 acagcgcgta cccgacgcca atgccatcgc cgatctggcg ttgcaaaact tcatagaaat
     9541 gcgagatttg gtggccgatc cggagttctt gcttcaaaaa cgaatcgcgg ccctgttggc
     9601 cgaacgctac ccacaggatt ttatgcccat gtattctatg gtgtcgttca gtcatgtgcc
     9661 gtatcgggag gcgctgcgtg ccggcaaaga gcaagccgtg ttgctgggtc gcattgcagc
     9721 attggagggg attgagagcc aattggaaca gcacccggag gtggatcgct tgttttggga
     9781 atggaagagg caggtttgac ttttttatac taagcccgac ataaaatttg aaataaatat
     9841 tgacgatatt ggggaaattg cgaacttggc aggctgtttg cgatgcaatc aggtataaat
     9901 gcaaaaaata tgagtgccaa tccctcttcc caatctgctg tccgcaatta catcagcggg
     9961 caatttagag ccgatgcagg tcgcgccatg gaggtcatca gccctttgga cggccaagcc
    10021 atttccgagg tgcccctccg gtcgcgaaac gcttgatgaa gccgttgccg ccgctcgcag
    10081 ggccttcaca tcctggtcaa aggccaccat caaagagcgg gtgcaagtgt tcttccgctt
    10141 ccgcaatctg cttgaaaaac ataagtcgga actgacggaa ctcatccgct tggaaaacgg
    10201 caaaacttat ggagaggccg aagccgaggt gctgaaaggc atagagttgt cggaatttgc
    10261 ctgctctctt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatagt ggcctccatc acgccgttta
    10381 actttccgct gatggtgccg ctttggacga tccccaacgc catctcgctc ggtaattgta
    10441 tgatactcaa gccttccgaa ttagtgccca tgagcgcgca acgcatcgcc gagatgctgc
    10501 acgaagccgg cttgcccgac ggcgtattca acatcgtgaa tggcgaccgc gaaatggtgg
    10561 aggccatctg cgaccacccc gatatagatg ccgtgtcgtt tgtcggttcc accaaagtgg
    10621 ccaaaatcgt gtaccggcgg ggcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcgccgcctc
    10741 catgtcgggt tgtgcaggtc aacgctgtat ggccgcatcg gctatggtgg cggtgggcaa
    10801 tacggagcac atcgtgcagc gcatttgcga agaatcgcag aaaatagtac ccggccaaaa
    10861 tctcggcgcg gtcattaccg gcgcggccaa agaacgcatt gagcgctaca tcacggaggc
    10921 cgaagctgcg ggtgctaaag tattggtgga tggcagaaac gccgtggttc ccggcaaaga
    10981 gggcggtttt tatgtaggcc ccaccgtcat agaccatgtg acgcccgata tggccatcgc
    11041 tcaggaagag gtgttcggcc cggtactcgt catcattcga accgacgaca tagacaaagc
    11101 catcgaaata gaaaacaaat cgccttacgg caatgcggct tccgttttta ccgaaaacgg
    11161 cggcttagcc aactacgtca ttgagcgagt gagcgccggg atgacggggg tgaacgtagg
    11221 cgtacccgta ccgagggaac ccttctcttt cggcggctgg aacgagtcgc gcttcggtgt
    11281 gggcgacatc accggcaaaa gctccatcgc tttctggacc aaactcaaaa aaatcacctc
    11341 caaatgggac ccgaaggctt ggaaagactg ggcgagctga tggggggaca ccgcgactat
    11401 gccgataccg ccctttcagg gctatgccgt gcgactcacg tcatagtccc ggagggacgc
    11461 catttcataa cgatgggcgc agtccatcga gatagaaatc actaaaacac agaatatcat
    11521 gggccaatcc ctcgtaaaaa actacatgca catcgtcttc agcacgaagc atcgtaaacc
    11581 attcatacta ccaaagttcg agaacgagct atatggatac atcggtggca tttgcaaaga
    11641 actcgactgc caacccatca tcgtaggcgg ccactacgac catgtgcata tcctttgtat
    11701 gctctccaag aaaatagccc tcatgaaact cgttcaggaa atcaagcaaa gctcctccaa
    11761 gtggttcaaa tctgtggatg agatactaaa ggagttctac tggcaagacg gctatggcgc
    11821 cttctccgtc aactataaag gcgttgatcc cgtcacacgc tacatcgcca accaacacga
    11881 acaccatcgc aataaaacct tcaagaggga atttcgcgcc ttcctgaaaa tgtacaacat
    11941 agaatatgac gaacgctatg tgtgggactg aatttgtggg aacacccgcg ctacgcgtca
    12001 cgaacaccgc gacgggcgtc gccacgtcgc tatgccgatt ttcgcgacgg gcatcgccac
    12061 gtcgctatgc cgatgtcgcc ctttcagggc tataccgtgc gaacgatggg gctgcccgta
    12121 ccgctcacgt cgaagctcga agggaagcca ctcagcaatg atgtaccgct cacgtcgaag
    12181 tcccgaaggg acgccatttc ataacgatgg gcgcagtcca tcgaaaaaaa acaacctaat
    12241 catgaataaa accaccgaaa tcaccgccag acaaaaaaag tacctctggc ccaaccacct
    12301 cctctactac accgagccgc tccccttaga gcgcggcgac ggcatgcacg tctgggacgt
    12361 agaaggcaac aaatacttag acttcttcgg aggcatcctc accacctgcg tgggacacaa
    12421 ccgcccagaa gtcgtacaag ccgtgaaaga gcaaacagaa aagctcatcc acagctccac
    12481 cctctacccc aatgaaaccc acgttgacct cgccgaaaaa atggccgaga tcacccccgg
    12541 ccggctccaa atgtcctact tcaccccctc cggcaccgac gccgacgaaa ccgccgtctt
    12601 cttagcccaa acttacaccg gtcgtcagga aatcgtggcc ctccggcacg gctacagcgg
    12661 tcgctccgcc ataggcatga gcctcaccgg acaacattcc tggcgcatag gcggcaccca
    12721 catagccggc atcaagcacg ccctcaaccc ctactgctac cgctgcccgc tcaaaatgac
    12781 ctaccccggc tgtggcgtag cctgcgcgca ggatatagag gaagtcatca aaacaaccac
    12841 ctccggcaaa atcgccgcct tcatcgccga acccatacaa ggcgtaggcg gcttcatcac
    12901 cccgccgaag gagtacttca aaatggcaaa agaaatcgcg gagcactatg gcggcctctt
    12961 catcgccgac gaggtgcaaa ccggctttgg gcgcaccggc gacaagtggt tcggcattga
    13021 acattgggat gtagaacccg acatcatgac gatggccaca agcatcgcca acnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtactccgtc attggcgatg tgcgcggcat
    13321 ggggctgatg cagggtttgg aactcgtgaa agatcgccag agcaaagaac cctctccgca
    13381 ggccacccaa caactcttcg aagccacccg ccaactcggc ctcctcatcg gaaaaggcgg
    13441 cctctacggc aacgtcattc gcatagcgcc gcccatgacc gcccgcgaag acgacattgc
    13501 caccgcactc gatctgttgg acaaagcctt cgcacgcatc tcatagccac aactcccagc
    13561 tcgtggctca tagctcgcag ctcaaaaaaa caccatgaaa aacgacatcg tagaactcac
    13621 cgaagacgtc tccgcatcgc ctttgtacag cgaagacttt gcgcccgtac ccgccgcagc
    13681 ccgcacctgg aaccgctggc acatcgcctc tatctggatc ggtatggccg tgtgcattcc
    13741 tacctatatg ctcgctgccg gactcattca gcagggtatg aactggaaac aggcgctctt
    13801 taccatcctg cttggcaatc tcatcgtgct cattcccatg atattgaatg cgcatgtggg
    13861 caccaaatac ggcatcccgc tgcccgtttt tatgcgcatg aatttcgggg tggacggcgc
    13921 ggtgctggcc ggcctgttgc gcggtttggt agcttgcggc tggttcggta ttcaaacctg
    13981 gattggcggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 cttcctgctc ttttggggga tgaatattta catcatctac cggggcatcg aatcgctgaa
    14161 aatgctcgaa acctgggccg cgcctttcct gctgctcatc ggaatcgggc tgttggtgtg
    14221 ggcttggatg cgggtcggtt ctatgggcga tattctggcc gcctcctaca tgctgggcgc
    14281 tgaatcgaag gtgagttttt ggaaaatctt ctggcccggc ctcaccgcca tggtcggttt
    14341 ttgggccacg ctttcgctca atattcccga cttcacacgc tatgccaagt cgcaaaaaga
    14401 ccagatcatt gggcaaatca ttggtctgcc gggtacgatg gtactctttt cgtacatcgg
    14461 cattgcggta acgagcgcca cgctgctgat ttacggcgag gccatctggg accccgtagc
    14521 gctcatggcc cgctttgact cgcccggcac ggtggccatt tctatgatcg gactgaccat
    14581 cgccacgctg accaccaaca tcgcggccaa tgtggtagcg cccgccaaca gcattgccaa
    14641 catcatgccc gacaaaatca gcttccgcat gggcggctac atcaccggca tcatcggcat
    14701 cctcatcttc ccctggaaac tactggccga cccgcaaggc tatatttttg tgtggctcat
    14761 tgcgtattcc gcgctgctcg gctcgctggc cggcattatg atctgcgatt atttcgtcgt
    14821 acacaaaacc cgcctgcatc tggccgaact gtacaagccc gatggccggt accggggctg
    14881 gaacctccgt gcctgggtag cttttacggt gggattgctg cccgtgctgc ccggtttttt
    14941 ggctacggtg ggcgctttac cggcgcaaag tgtgagtgca ttctggaccg atttgtattc
    15001 ttatgcctgg ttcgttacct ttttgttgtc gtttgttgta tattggctgc tcagtaaaat
    15061 gagggaaaaa taattcacca tgtccattct cattcgcaac ggcaggatcg tcaccgcctc
    15121 cgaggactat gttgccgaca tctatatcga aggcgaaacg gtacattcca tcggcaagaa
    15181 cctgcccatg caagccgacc aaatcattga tgccggcggc atggtggtca tgcccggcgg
    15241 tatagacccg cacgttcacc tccagatgcc ttttatggga acctggtcga gcgacagcta
    15301 cgaaaccggt accctggnnn nnnnnnnnnn nnnnntacca cgatggtcat tgattttgtg
    15361 ttgcaaacgc agggcaaatc gctgtaccac gcattggaag cctggcgggg ccgttccgac
    15421 ggcaattgct acggcgacta ttcattccac atcgcggtga cggattacaa caacgacacc
    15481 cgacaggaaa tcggatcttt ggtagagaat gaaggcatca cttcgttcaa aactttcatg
    15541 gcctacaaag gcgccctgat gatagacgac ggccagatgg tggacctcat gcaggaggtg
    15601 aaacgccacg gcggcatggt aaccgtacac gccaccaacg gcgatatgat agacaagctc
    15661 attgccaaac accgcgccga gggcaagctc tctccgctct accactacct ctcgcagccc
    15721 gaagtgaccg aagccgaggc ctccgggcgc tttgccgaca tggcccatta caccggcgtg
    15781 cagggctaca ttgtacacat gacctgccag ggcgcgctgg atgccgtgcg tcgcgccacc
    15841 ttccgcaacc agaaagtata tgccgaaacc tgcatccaat atttggtgct cgatgcatcg
    15901 ctatacgaaa aagaccccga aggcgccaaa tgggtgatga gtccgccgct gcggcagaaa
    15961 aaagatcagg aagcgctttg ggcgggcata gatcagggct tggtacaatt ggtagctacc
    16021 gatcactgcc ccttcacctg ggagcaaaaa atgatgggca aagacgattt ttccaaaatt
    16081 cccaacggcc accccgccat cgaacaccgc atggaactgc tgtggtcgga aggcgtggcc
    16141 aaaggtcgca tcagcgccaa taaatatgtg gaactcagct ctgccaacgc cgcccgcatc
    16201 ttcgggatgt acccgaaaaa aggctgcatt gcgcccggct cggatgccga catcgtcatc
    16261 ttcgatccca acgagcgcca taccctctcg gccaaaaccc accacatgaa cgtggactac
    16321 tcggcctacg agggccacga ggtacagggc aaatgcaaaa ccgtgatcct ccgcggcaag
    16381 gtagctgttg acgagggtaa agtgctgata gagaaggggt acgggcagta tgtaaagaga
    16441 aaagcgatga aatgaaattt atgttctatg aaacaggaag aaaaagaaaa actggaggaa
    16501 gagattctta tgcttcgcgt actgttagac tttcatgttc agaataagag ttatgtaact
    16561 atgggcgaaa gagattttga ggagtacgtc aatgctgttt tagatcggtt gaacgaactt
    16621 gaaaattttt taaaagaaga aaatgatcgc ttatgaaaaa atatgctcac atccatgaag
    16681 tggtaagtcg cctgacagat gacggagcct tgcgcacttt ttttacgttg tatctgatgg
    16741 cagaatccac atccgagaga gtacggctca atgagcaatt ccaagtcgag atgtcttcac
    16801 tgacttcgtt agaacagaaa ttgatgaaag cagaactcnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattttg aaacgataac acatgccaag
    16981 aatcatcaaa tccggcctca tccaaatgag ccttcccctc accgaagggc agggcagcat
    17041 cgaggaaatc atggaggcca tggtgcaaaa gcacattccg tacatcgagg aagcgggccg
    17101 gcaaggcgtg cagattcttt gtttgcagga aatcttcaac acgccgtatt tctgtccggg
    17161 acaagacgcc gcctggtacg cttccgcaga atcggtgccc ggccctacca ccgagcgcat
    17221 ggccgagtac gccaaaaaat acggcatggt catcattgtg ccgatctttg aaaaggagca
    17281 ggccggtgtg ctgtacaata cggctgcggt gattgatgcc gacggaactt acctcggcaa
    17341 gtaccgcaaa aatcacattc cgcacacttc cggtttctgg gagaaatttt tcttcaaacc
    17401 cggcaacctc ggctacccgg tttttgaaac ccgctatgcg aaagtgggtg tctatatctg
    17461 ctacgaccgc cacttccccg acggtgcgcg tatcctcggc ctcaacggcg ccgaaatcgt
    17521 ttacaaccct tcggctacag tggccggctt gtcgcaatac ctgtggcgat tggagcaacc
    17581 cgcgcacgct gccgccaacg gctacttcat gggctgcatc aaccgggtag gaaccgaggc
    17641 gccctggaac atcggaaagt tttacggctc gtcttatttt gtggacccgc gcgggcagat
    17701 ttttgcgcag gcttcggaag acaaagatga gttgctggtg gccgagtttg atctggatat
    17761 gattgaccag gtgcgcagcg tgtggcagtt tttccgcgac cgccgaccag agacgtatgg
    17821 gaaactgacg gaattgtaaa ggctatcggg caaagagttc attcatcggt atttggatgc
    17881 ccagattggg atcggacaaa acgtcgccga ggttgaaggc ttcatgcacg cccggcgccc
    17941 gaaacacata aatgctttgc agcgtgggca atacgatcca acaggatttg acgccaaaag
    18001 agaaataatc gtaggttttg tctatcagcg tttgcaacac ttgagtaggg gagaggattt
    18061 cgatggtggc caaaggaggg tctttgcgtt tgattnnnnn nnnnnnnntt gattacatct
    18121 tgcgaaaaat caatgggttc ttttctataa acagaaatgt caggagtcat gcttttgccc
    18181 ggtgtgtcta atgtaagctc tggaaacgcc tcatataatt ctttgtaatg cttgacaaga
    18241 tagacagtca gccgggtctc tgtaactgca tgtaaaaagc tgggcaaggg ctttcccctt
    18301 tcaatttcgt aatcggatag accagatgtg ttcgcagtgt ccatgccttt cagttttgta
    18361 caaagatgac ttttttctga aaaccatcct aacgaaaaat tacctttgtc acgatgtgat
    18421 cttcttcatc acctaattgc ttttgatcat gccggaatac ccaacacccg ccaccgcgca
    18481 agacttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccaccgg catagacatc
    18601 ccgctgttca tcaggcagat acacacgcac aacgcagagg gggcagcccg aacgatttac
    18661 gattccaatt actttggcaa tatctgcggc aaagtgtgcc ccactgaggt actttgcgaa
    18721 ggcgcctgcg tgtataacca ccagcatgtg aagcccatag aaataggccg cctgcaaagc
    18781 tacgccacct cccaagtggt gaacgagcaa aaaagactgt tcagtccggc tccggccaac
    18841 ggcaaaaaag tggccgtcat aggcgcggga cctgcgggca ttgcctgtgc ctgcgagctg
    18901 cgattggccg gctgcgaagt agaggttttt gaagccaaag agcagccctc cggtttggta
    18961 ttgcacggat gtgctcccta caaaatagcc aatgaggacg ccctcaacga ggtggcctgg
    19021 ctgcaaagtc agttcgggtt caacattcat tacaaccaca acatcgaaag ccgcgagcag
    19081 tggcaggcgc tcgaacaaca gtacgatgcc atctttctgg gcatagggct gggcgctacg
    19141 caggctgccg ggctgcgagg cgaagacctc gaacatgtga gcggcgctac cgaatacatt
    19201 gagcaaatca agctcgaccc gatgagcgtt cgcgtggggc gcaaggtggt ggtcatcggc
    19261 gggggcaata ccgcgatgga tgccgcctcc gaatcggcgc gattgggcgc agaggaggtc
    19321 acgctggcct accgccgccc caagccggaa atgaaggctt atgagtttga atacgaattg
    19381 gccaagtcgg tgggcgtcaa gggtttgttc aatgtggcgc cggtggccat tttgggagat
    19441 cggcaagtgg agggcatccg gctggcagca acccgcgtgg aggcagggcg cttacagttg
    19501 gttgatggtt ccgagttcga tctcgcctgc gatgcggtca tcttagccac cggacaggaa
    19561 aaacgaagcg ccctcgtggc ccaaatcggc ggcatcgaga cggaccggca ggggcgcatt
    19621 gtcgtacatc cagagacttt ccaaagctcc cacccaaagt attttgccgg tggcgatgcg
    19681 gtcaatggcg gcgcagaggt agtgaatgct gccgcgcatg gcaaggccgc cgcgagggga
    19741 atcctggggc ggttgaatgg ataagtgaca attacaaaat aaaacgacat gtttgtacac
    19801 cttgaaaacg tgccgcgctt ttacaaaaac tatgtaaagc tggtagaaga aaccgacatc
    19861 ctgcaagcgc tgcgcatttc gggcgagcgc atgatggagg tattggcctc cctcacagaa
    19921 gcccaacagg accatcgcta tgccgagggg aagtggagca tccgcgaatt gctttgccac
    19981 atcatagatg ccgagcggat tttcgcatac cgcgccctcc gttttgcacg caaggaccaa
    20041 acgcccttag ccggctggga ggaaaacgac tacgcgccgg aagccaacgc cgagggaagg
    20101 agcctgtctc acatcatcgg agaaatgaag cgcctgcgcc aatctaccat tgacctgttt
    20161 gaaggattct cgcccgaaat gttggatcgc aagggcattg ccaacaacaa cgaaatatcg
    20221 gtggccgcgt tgggcttcgt cattgccggc cacgagatac accatcgcaa catactgaca
    20281 gagcgctatt taacacattc agccaccctg taaagccatc gaaaaatgcc tgatctttct
    20341 gccaatctcg ccgggatcaa atcccccaat ccgttttggc tggcctccgc gccgcccacc
    20401 aattcaggct atcagattat gaaagccttc gatgccggct gggggggagc tgtctggaaa
    20461 accctcggcg tgccggtggt caacgtatcg agccgctacg gcgccatcaa ctaccgcagc
    20521 aaccgaatgg tgggcttcaa caacatagaa ctcatctcgg accgccccct gcgcgacaac
    20581 ctccgcgaaa tagaggaggt gaaaaaatac tttccgcacc acgccgtcgt ggcctcgctc
    20641 atggtgggat cgcgcgcaga gtggcatcaa atcgtgaagg acgctgaaaa tgccggcgcc
    20701 gacgggctgg aactcaattt cggctgtccg cacggcatgt gcgagcgcgg tatggggtcg
    20761 gcagtagggc aggagccgga ggtgctgaaa ctcatcactt cctgggtgat ggaggtggct
    20821 accgtgcccg tcattgtgaa actgacgccc aacatcagcg acatcaccga gccggcgcag
    20881 gccgcagcgc agggcggcgc caatgccatc tcgctcatca ataccattca aagccttgtg
    20941 ggcgtggaca ttgacaactt tgtgtcttac cctgtggtgg acggcaaaag caccaacggc
    21001 ggctactgtg gcccggcggt gaaacccata gcgctgaaca tggtgaaaaa ctgcgcgcag
    21061 caccccggcg tgcggctgcc catttcggcc atcgggggca tcgaaaactg gcgcgacgcg
    21121 gtggaatatc tgctgctcgg cgcctccaac gtgcaggtgt gtacggcggt gatgcactac
    21181 ggcttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgattcccgg attggaaaac
    21241 tacatgcgcg acaagggttt ccagcgcttg gacgacttca tcggcaaggc gctgcccaat
    21301 gtgctgcatt gggaagacct caatctgcac tacgatgtga aggcgagcat caaccccgat
    21361 aagtgcatcc aatgccagct gtgctacacc gcctgcgaag acggcgcgca tcaggccata
    21421 cggatccccg aagatggcag ccgcattcct caaatcatcg aagaaaactg cgtgggctgc
    21481 aacctctgtt ccctggtgtg tccggtggaa aactgcatca cgatggtgcg caaagacgac
    21541 ggcagccgcc atgaaaactg gaaagacttt gtggttgccg gaaaagcgcc cgaaacgttt
    21601 gacgacgagc gggccggcgg gctgggccat tgggtgccgg cgccgacgga tgcgttgaag
    21661 aagagagggt agggggcgat taaattcgat tgggatacca tccagttgag gaatggcgag
    21721 atatgttttc ggtacgagtt tccagtaaga tcgccatctg tgagacaaca aaaaagtgat
    21781 tggttaccaa tttaaagcta tcttaaactc aggcagtttc aatacagaaa ctgttggaaa
    21841 tgggatggtt tctaaaacct tgaaatgcct gtcatgtgta accaaataat ggacattggc
    21901 agagattgca agatctgcaa acttgttgtc ttcgggatcc gcttcgatca aattccatct
    21961 aaagaaaggc tcctgaaata caacgttggg cgcagttgtt agtattttca aaacaatgtc
    22021 ggcggttaga ggggagtaga attgtgcaag ttgttcttca tattcatgaa gtatttcatt
    22081 gctgattacc cattgaaatt tttgagcgac aaatgcctcg tacagccaat actcggggtt
    22141 tttgcgagga attgaaacaa tgaggcagtt ggtgtcaatc actacattca cggtaatgtg
    22201 cgttgttgga gttcaagtct cgtttcaaag tcttcgggtt gaatattctt ttgggcgatc
    22261 acacgatcaa gctctttttg gagagcatca tcataaaaag acagcaatat tttctgtagt
    22321 gcctgaaatt cttcttgctc catctttcta ctaaagaggc gaatgagtgt gagttgagct
    22381 tcnnnnnnnn nngttgagcg gaacgggttg cataagctgt actttgaatg aatttaacag
    22441 cattagcctg ttttgggttg acgctgtatt tgtcagatcg cgtgaggggg ccgaaggtag
    22501 tgacgacgat aggaggagcg actgaggcac ccgacggcac accgtgaggg tggtagtgtg
    22561 ccggcacgcc ccatttcaat tccgcctcca tttcgttgaa acattccgac ggttggtaga
    22621 aatttaaagc ccggctgaaa acagcatccg ctttcccgcg tttcatgctg cgacaatcaa
    22681 ccgacaaaaa aacatgcaat ttcttacccg acttattctg ctgcccctgt tgctggcctc
    22741 cggcgcgctc atggcgcaac aacgctacac catcagcggc gaaatcaaag atgccggcaa
    22801 cggcgaaacc ctcataggcg ctaccgtgac cgtgagaggc caaggcaccg gcgccaccac
    22861 caacgaatac ggcttttact cgctgaccct gcctgccgcc gactcggtgc tgctggagtt
    22921 cagttatgtg ggctaccaga ccgagctgcg gagcgtatcg ctgcaaaagc cgctgattat
    22981 caacgtagaa ctcagttccg gcatacaaat ggaggaggtg gtggtgaagg ccaattcctt
    23041 ccgcgagaaa gcccgctcgg tggaaatgag cgtggaggaa atcaaagccg ccgacgtgaa
    23101 actgctgccc gtgctgctgg gcgaaagcga tattttgaag gcgctgcaac tgaaacccgg
    23161 tatcccttcc ggctcggagg gcaccaccgg cctgtttgtg cgcgggggcg gcgccgacca
    23221 aaacctgatc ctgttggacg aggcggtggt gtataacgcc aaccacctgt ttggcttttt
    23281 cagcaccttc aacaccgacg ccgtcaaaga cctcaagctc tacaaaggcg gctttccggc
    23341 gcagtacggc ggcaggctct cctctgtgat agacgtgaaa ctgaaagagg gcaactccaa
    23401 aaaatgggcg gctaccggcg gcatcggcct catcgcatcg cgcctgacgg tggaagggcc
    23461 gatagagaaa gacaaggcct cctttatgat ttccgggcgg cgcacttatt tcgacctcat
    23521 cacgcggggc gtcaactcgg ccaacaaaga caacgccaac tattcgccca ttcccgacta
    23581 ctatttttac gacctcaaca ccaaaatcaa ttacgacctc ggcgaacgcg accacctcta
    23641 cctcagcggc tatttcggca gggacgtgtt tggcttcgac ggcgactttt tcaatttcaa
    23701 tttcaactgg ggcaacgcca ccggcaccgc ccgctggaac cacgtcttca atccccgcct
    23761 ctttgccaat accaccttca ccttctccga ctaccgctac aacatcagca acaaactcac
    23821 cggattcagc ttcgacctcg gcagcagcat ccgcgacgcc gccctcaaaa ccgacttctt
    23881 ctacgccgtc aatccccgcc acaccctccg tttcggcgcc gccctcaccc accattattt
    23941 ccaggtgggc cgcctcaaag ccggcaatcg cgacggctcc atcgccttct cttccggtca
    24001 ggatttcaac gggctggaag gcgccctgta tgtggccgac gaactggacc tgagcgaaaa
    24061 aaccaagctc aactccggcc tgcgcctgag cggattcacc aatggcgacc gcttctatgc
    24121 caatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggtacccctc taccgaccgc gtgccgccgc
    24301 agcgctccga tcaggtggcc gtaggattgt cgtatttgct gggcaatcag taccttatca
    24361 ccctcgaagg ctaccataag tacctccaca accaactgga atttgtggac ggcgcccggc
    24421 tctttgccaa caacaacctc gaagacgaat ttgccatcgg gcgcggccgc tccaacggcc
    24481 tc
//
LOCUS       DAORLF010000015        24348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1141_length_24348_cov_1.890298, whole genome shotgun sequence.
ACCESSION   DAORLF010000015 DAORLF010000000
VERSION     DAORLF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24348
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_24348_cov_1.890298"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PK198_02535"
     CDS             <1..968
                     /locus_tag="PK198_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37640.1"
                     /translation="NGANNLNITAVAVPGVCGAPGQIHVGITGGTAPFTVSWTGPQNG
                     SASTSNNFYNILNLTSGTYTVTVTDANGCTRTQTVILNNGANNLQVTLTPVPGICVQT
                     GQIQVGIAGGTAPYTVSWTGPQNGMATTNTGSYTIMNLPSGAYTVTVTDANGCTATQT
                     TTLNNGGIVTLTAASANGICGQNGSIQLTMTGGAAPYALSWTGPVSGNATANGGAYTI
                     SNLPSGTYIITATSGANCVSTQTSTLNNGSVFNMTVVSNPGFCNINGSVVLTFSEGQP
                     PIAITWSGPVNGSATTSGNSYTLSNLPTGTYTITATTAQGCTDTE"
     gene            979..>1323
                     /locus_tag="PK198_02540"
     CDS             979..>1323
                     /locus_tag="PK198_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37641.1"
                     /translation="MLTVTGTPVNGLCGEPGSVTLSMTGGTGPYTIAWTGASSGSTTS
                     ANALVTIPNLGTGTYNFTVTSANGCVQSTSASVQVTESDIDITSTANTGNCGESGSIQ
                     VIISGGNPGYVIN"
     assembly_gap    1324..1349
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1350..>2434
                     /locus_tag="PK198_02545"
     CDS             <1350..>2434
                     /locus_tag="PK198_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37642.1"
                     /translation="TTSGSTYVINGTTAGDYNISVTDAQGCTETTSVTLNNPEADLDI
                     LLSRMNGICSGPGAVTVSIISGDAPFNIVWTGPVNGSTTTSNSSFTINNAPAGTYTVT
                     VTDVDGCTDTGTITVTTTPNVVATTIAQNGVCGMPGSIILNLVGGTAPYTIAWTGAAS
                     GSVTSQTTTRTFNNLGSGTYNFTVTDANGCVATTTAVLNNSGNIFTINAFPYHGTCGD
                     LGRIRVNMFGGTGPFLIIWVGPSSGSATTSATTYDILNLPSGMYFVSVTDAAGCTGTD
                     VEMITNSNTSSINVSVTAINGLCGDLGSIWITVLQGQGPFVITWTGPQSGQITTSSTD
                     FDIPNLPNGTYTVTIRHVNLCERTYTV"
     assembly_gap    2435..2491
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2492..>3530
                     /locus_tag="PK198_02550"
     CDS             <2492..>3530
                     /locus_tag="PK198_02550"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37643.1"
                     /translation="PGSITVNIAGNAPPYIINWNSAIGSGTQTVSTNTFTIPNLFTGN
                     YTVQVVDANGCSETHTRQLYNEAGTFIMTLTPNVGGCNNQSSIQASISGGQAPFLLNW
                     TGPSSNGSVTFTTSTYNLFGLAPGFYMIMITDANGCSQWETVQVSYASSPPITAFAFT
                     TNLLTANFTNQSSPGTYLWSFGDGTTSTAANPVHTYAQDGTYTVCLIVSNACGSTTHC
                     ANVTVTIPNNMVILDIGENSGAAGGNVLVPVRIRNCNLLVSLAGSLTVQNPSVASIAG
                     VAPAAIAPQFNPVNKTFNYYANNGQGVSLQQNQILFYLIVQLTGTPGQSTNLSFANSP
                     LAVEVGSMVNGN"
     assembly_gap    3531..3547
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3548..>3906
                     /locus_tag="PK198_02555"
     CDS             <3548..>3906
                     /locus_tag="PK198_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37644.1"
                     /translation="ITYTGSVSISTAVQVSGDVYTYWDAPIPDAEVRISNTGMDMMEM
                     TDDAGRYMAPDVPMGEMITITPSKNGHPANGLSTYALFIGQRFILGMEPPQVASPYQV
                     IAGDANCNGAFTTLDLF"
     assembly_gap    3907..3934
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3935..>4401
                     /locus_tag="PK198_02560"
     CDS             <3935..>4401
                     /locus_tag="PK198_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37645.1"
                     /translation="GVRDSFANCPAWVFVAANSNLPESDFDAYNVFPYANCDSMMVTG
                     DALADFVGVKVGDILGHANPANFTNGGGIEPRSAAELVLTTNNRQAQQGDLISIPVRS
                     SNFEDITNYQLGLWFDTQYLTFEGFDPAASGALSSVVAGTTQTGNGRLSVSWF"
     assembly_gap    4402..4542
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4543..4876
                     /locus_tag="PK198_02565"
     CDS             <4543..4876
                     /locus_tag="PK198_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37646.1"
                     /translation="ERKGIILNIENQAVTSANEPLEGKGTFRLHQNAPNPFVAETSIS
                     FDLPEASDVELLITDQLGRTVRILRGQYPQGRSTMDFKAEELPQGVYRYTLKAGEFSA
                     TRTMILVK"
     gene            4939..>5739
                     /locus_tag="PK198_02570"
     CDS             4939..>5739
                     /locus_tag="PK198_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRF37647.1"
                     /translation="MIHLLRRCCLLLAVWQAPFFPSAQTIQLSMPDTTVLAGDTLSYP
                     ITATAGFNGVVSAQFSIQWNPSVLRYIGHLPVDLNQIAVGETSAAQGALRFSWFDVGG
                     QGITLAPGSQLVRFRFRAEGQPGDSTPVVFTNEPLRIQIFRAGSTPGLFDSLVLVQDT
                     GLVKIARVWQPTAVSQNPTCFGMNDGTASVNAADAPAGAQFLWTGPNGFSSSSPVLSD
                     LGPGAYNLVATSSQGEVLFETTITLNEPPPLQPDLGDDLQLCPGESSTL"
     assembly_gap    5740..5760
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5761..6623
                     /locus_tag="PK198_02575"
     CDS             <5761..6623
                     /locus_tag="PK198_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37648.1"
                     /translation="LWSDGTTAAQLPINQTGTYSVTVNDAQGCTASDTIAVNVSADVQ
                     IVVENDRLSICPGDSVQLRVSGGETYRWTDPSNTLSSTTIPNPVAKPQQSSSYTLIAS
                     NECASDSIQFSIFVHQTDASAGPDTCIQRGQELRLYARGGVRYAWDRGEYPLNSYSSS
                     DPLSTPLSSTVYRVEIVDANGCKTTIEVPVTVVDGAPDILLINMITPNGDGRNDVLEF
                     QELGKFTNNALKVYNRWGDLVYQKLGYQTDDERFDGTWNGKPLPAGNYFYVLSFSEVH
                     YKQTLTIIRD"
     assembly_gap    6735..6779
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6780..7568
                     /locus_tag="PK198_02580"
     CDS             <6780..7568
                     /locus_tag="PK198_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF37649.1"
                     /translation="TSYRQQWLGFDNAPRTAAVAIQYPLPESNSALGGYFMYDQINPL
                     VVNHFTFQYAYKLRFGDRKRRQLSLGIAANVQHFLLDARDVVVNHADDPLLPVGEYNT
                     VGPNASGGIFYTSDARQSDNKGAWYAGAAIQQAVPWNVVFDDNDSPANLRHTWHGNAI
                     FGGRFAKGGVILEPALWVHYAQPGIVNGAFHLRAESRDALWGGLSYSSNQTLGIQAGV
                     VVKKGFKEFSHLRIGVAGTFNIGTFGQFRGPGYEFLMAYRMAQD"
     gene            complement(7580..>9745)
                     /locus_tag="PK198_02585"
     CDS             complement(7580..>9745)
                     /locus_tag="PK198_02585"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF37650.1"
                     /translation="GVIPEAARTELVEYLASKSMEERVRGIIHNLLQQSTPPDVASAA
                     YEEYRFNAALNALAITSNSKEKNLLKAELQGLHTAGHPVDEVAFKYVEEPPSRTAFTI
                     PPWLRKLAEPAADRLRPAAFDLLWAIPLWAFFTAFAWWYNPPFDVCADGESVVYQNRA
                     LCIHNPTDQLYYNEMLVGDLIQQKNMAATDSLIEASRALFPACISADTIAFLKNVAVY
                     LYNEGVQEFSRLQIDAGYQPVAGDPWPESACFWFAKAYESEIAARGMLDTDILSAAVR
                     CKKQDEPATALPDPDGELKPIVVRGIVQAAATKRPLAKVKVSAGSFTDYSDKTGAYTL
                     SFPAATEGSLVTLQFTRQGYAPAQLTVKLQPGATPPRVEMTQEAPVKDERLRTFRKGE
                     LVGLQDAKGNVLAPAEYYSIDYDAAGNWYKVEQRLRGSSIFGYLNSAGSVAIPIRYRN
                     IGPLREGLIRAEMESGWGYINMAGQTVIPFQYLRAGDFSGGRAEVSLNSKGETSTFVI
                     DKSNKCVANCPQGNQTPVPNTPTVLNYTLPMSLYFDENIPEKTATSYEDLLKAYLARR
                     DELRRNFSNVVQQNNVSATDDIAGFFNQASNAFETLTQATAEIDSKLKTGLAPGESIV
                     VRISGFAGDQERDASQLAARRAESVYIWMSNYGRGALQSYLKSGQLRIIRDGAQDGGI
                     RGKEAADWLRDARRRYAQVTISVQNEKSEQNLRAPAKKG"
     assembly_gap    9746..9779
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9780..11731)
                     /locus_tag="PK198_02590"
     CDS             complement(<9780..11731)
                     /locus_tag="PK198_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37651.1"
                     /translation="MSAMTDTRLHILMSELTAALEADGYDMGTGKHLQVQELLRRLPA
                     DIAPERLSALLCPLFARNRQEQEDFYRLFEQVWQRVVLLSEASAERSDSARERQLARV
                     WRNLLLLMTAGFSFLGGFLLDVEVFSSWRNPTIPIVVLSLLAAGYFVRRTVASPRKRK
                     WWLLAQILAIVAGVAVKQQVGPKPPPPLLPQYVERVLRPGDTLTERISIPQGDSLLMA
                     LLGNGASSGSDSVFGAYQVSPQGLFTYIVRDTYDFFRDTILVEAWLASARRDTTFFVI
                     SLQLGIEDLDLVKPDTSLALPSTKNVPFPRDMRELLPDPGREYAIHLYTQYAWVVKSA
                     LTVLSALGLLALLQWRERRRRFLTAVTQQKGRSGPPYHFRFRIEQPGAIVPGDSAAQL
                     LFLLRRRIRGDAMQLDIPASIRATINRLGMADFRYKRLTNPPDYLLLIDRQSPNDHRS
                     ALYDWVCRYFQTQEAPLARYFFEGDIRLCFDEKNPEGIPLRELQHRYGNARLIVIGHG
                     HQLLSPLTGRLERWVSMLTDWRRRAMLSPIPVSVWGRREQRIEEVFYLLPMSLQGWSA
                     AVEQLDATEPIHTSEWLARLEDIQREPIVLEGDLMTSLRKHFSEPMIEWIAACAVYPL
                     LQWELTLALGRQLSPPGATLLHFDQLR"
     gene            complement(11766..12305)
                     /locus_tag="PK198_02595"
     CDS             complement(11766..12305)
                     /locus_tag="PK198_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37652.1"
                     /translation="MNKYDTIYEHLLEEFTVEEIAGAYIFSGDLEKNEQTEIEEEFRK
                     LRAKALLERTEEQRLLSELMRMKLLMRDYFERSGFEEAFSFSKQLEQYIKILGRSHKE
                     FAEDVDLHPTKLSRLLNDRENPNVELTYRLETHCGNIIPAVYWWKLLAKRMEENVRTD
                     DEMRQTEKQRVRNQLRFSA"
     gene            complement(12298..12771)
                     /locus_tag="PK198_02600"
     CDS             complement(12298..12771)
                     /locus_tag="PK198_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37653.1"
                     /translation="MKVTRKASGSLVEVFIEEATSKDIAKIRKHPGFSFDWEQELGHE
                     VYKLYLKEDENEILGLLSLKDYPEELRVHINLIEITKTQVGKEKTLDNIAGCLIGFAC
                     ASAFKRGYGGFVSLLPKTRLIHHYQDKYGFRQYGRLLAVEYESSKQLIDKYIGDE"
     gene            12909..14234
                     /locus_tag="PK198_02605"
     CDS             12909..14234
                     /locus_tag="PK198_02605"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRF37654.1"
                     /translation="MKYLASLCLLIVMSTAQAQHHPLLQSGPMPGYTDMLETTLWVQT
                     KAPAKVHIAYWNAAGKADTLLTEPVTTQKHSAYTAKLVADKVQPGQKYNYQLRIDGKA
                     VSLPYPTQFQTLPLWQWRADPPNFTLATGSCNYVNEPDYDRPGQGYGSDHHIFKAIHD
                     RRPDVMLWLGDNTYLREPDWNTRTGILYRNTHSRSLPELQPLLASTHHYAIWDDHDFG
                     PNDSDGSFPFKDLTYEAFQLFWPNPSFGVNGQKGITTFFQWADIDFFLLDNRYFRTAN
                     NRKTGDMQILGAEQIDWLISALSNSRSPFKIVAIGGQVLNTAPVHENYIHFHAAEREF
                     LLKRIEEEKIKGVIFLTGDRHHTEMSKLVNKAGNTVYDLTTSPLTAGTARASEDEPNI
                     YRIPGTMVVGKHNFSTLEFSGPRTARVLTIRVFDAEGAQLWQREIKAGE"
     gene            14362..14592
                     /locus_tag="PK198_02610"
     CDS             14362..14592
                     /locus_tag="PK198_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRF37655.1"
                     /translation="MKKQIALLSYFLIAQILPLAAQSADTDFMRSRGKIFVVVAVIAA
                     VFIGIVLFMVFLERRLTKLENQINEHEPTDRR"
     gene            14570..16000
                     /locus_tag="PK198_02615"
     CDS             14570..16000
                     /locus_tag="PK198_02615"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRF37656.1"
                     /translation="MSQQTVVSFYDSVTNFFNKAAQHTGLPEGLLEQIRVCNAVYRML
                     FPVKMQDGQVKVIEAYRVQHSHHRTPTKGGIRYSNHVNQEEVMALATLMTYKCAIVDV
                     PFGGAKGGVKINPWDYSPEVLERITRRYTTELMSRNFIGPSIDVPAPDYGTGEREMAW
                     IYDTYRTFKGADIDAAGVVTGKPVTQHGINGRREATGRGVYYGIREACNIPEDMKRLN
                     MTTGLKGKRLIIQGLGNVGSFSGTISQDEGGAIIVGVGEMEGAIYNKDGFDINDVLKF
                     RKETGSILNYPGAISIPKEQRETILEYDCDILVPAALEDVISYKNAPNIKAKIVAEAA
                     NGPITSRGEEMLLEAGKIIIPDVFLNAGGVTVSYFEWLKNLSHMRFGRLEKRFDQQRL
                     ENLVGLVERTVGREISDEEKRMIARGADEIDLVRSGLEETMINAYHQIRDVMVDKPKV
                     TDLRTAAFVSAINKISSDYMSLGIFP"
     gene            complement(16081..>17167)
                     /locus_tag="PK198_02620"
     CDS             complement(16081..>17167)
                     /locus_tag="PK198_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF37657.1"
                     /translation="VAGGAYLPIMSDEALIVDGTGSIFLAGPYLVKAAIGEDIDKETL
                     GGARTTAEISGITDYRMPDDPTCLSTIRDLVDKLGTFPKAGFDRAEPRLPKENPSEIF
                     GIVPENSAKPYSMTELLRRIVDDSELTFYKKDYGKTIICAYARIDGWSVGIVANNREV
                     VRTPKGEMQVGGVIYSDSADKAARFIMNCNQKKIPLVFLHDVTGFMVGSRSEHGGIIK
                     DGAKMVNAVANSTVPKISIVVGNSYGAGNYAMCGKAYDPRFIFAWPNAKIAVMGGEQA
                     AKTLLQIQTAGLKAKWQQLSPEDEAHLLHDIKSKYERQTTPYYAAARLWVDEIINPAD
                     TRRAISMSIEAADNAPVEKFNVGVFQV"
     assembly_gap    17168..17245
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17246..>17649)
                     /locus_tag="PK198_02625"
     CDS             complement(<17246..>17649)
                     /locus_tag="PK198_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF37658.1"
                     /translation="IHQGGGKAKIEKEHSLGKLTARERVNHLIDPDSYFMEIGAFAGF
                     EMYEEHGGCPAGGVVVGIGYVRGRQCVIVANDATVKAGAWFPITGKKNLRAQEIAMEN
                     RLPIIYLVDSAGVYLPMQDEIFPDKEHFGRIF"
     assembly_gap    17650..17721
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17791..18321)
                     /locus_tag="PK198_02630"
     CDS             complement(17791..18321)
                     /locus_tag="PK198_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37659.1"
                     /translation="MSGKNLFALLLLLFAATMPAFAQLAKPAETEAEFEKNYQWRIKQ
                     ERLDGIYIPKDLVNAFEELNRLIEPSAKATFKSATEDEVVHKLFFSLGRWISQKWGFY
                     EGSRFSHYLKSLGVSYPEDMSQIVIVSYHRYLNKKPLEIKVQIEAIKEKRQQENEERM
                     KKGTVIELERKKVNKN"
     gene            complement(18396..19298)
                     /locus_tag="PK198_02635"
     CDS             complement(18396..19298)
                     /locus_tag="PK198_02635"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HRF37660.1"
                     /translation="MGARAAEIAIERAGISKRDVDFIIFATLSPDYYFPGCGVLLQRE
                     LGITDTEIPALDIRNQCSGFVYGLSVADQFIKTGMYKNILLVGAEIHSMGLDFTTEGR
                     NVTVIFGDGAGAVVLQPTDEEGKGVLTTKLHSNGTYAEKLAMINPGAHGGYHSEKMGE
                     DIDWGYPETEYGQMFITQHMFDNGQIYPYMDGPFVFKMAVQKFPEVIMEALHSQSLTA
                     ADINLLVPHQANLRISEFVGKKLGLSPAQVWSNIQRYGNTTAASIPIALCEAVEAGAV
                     KAGDLVCLAAFGSGFTWGSALIRW"
     gene            complement(19267..19455)
                     /locus_tag="PK198_02640"
     CDS             complement(19267..19455)
                     /locus_tag="PK198_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37661.1"
                     /translation="MLRTKIAGIGSYVPEKTVTNHDLQKVMDTTDEWIQERTGIRECR
                     KTQRNVHHDGRPRCRNSH"
     gene            complement(19526..>20409)
                     /locus_tag="PK198_02645"
     CDS             complement(19526..>20409)
                     /locus_tag="PK198_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRF37662.1"
                     /translation="AFEACCRSAESRGMRVTGSEIVGLLPLQVLLDAGRYFLRKQKRS
                     AGVSEEELLRIAVRSMGLDDLGPFDPKQKVIEYKLQSEASNPLIAKNLVQFADETASE
                     SPAPGGGSISAYVGALGASLAAMVANLSSHKRGWDERWEEFSDWAVKAQDIKNQLLAL
                     VDEDTRAFNAIMNAFQLPKGTPDEKAARSAAIQSATRFAIETPLKTMELALASFGPVR
                     AMVEQGNPNSVTDAGVGALCARAAVHGAGLNVLVNTGGLKDKSFAEQAVQQARAWMEQ
                     ADLMEKEIMALVAEKIS"
     assembly_gap    20410..20429
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20430..>20899)
                     /locus_tag="PK198_02650"
     CDS             complement(<20430..>20899)
                     /locus_tag="PK198_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRF37663.1"
                     /translation="AEWAKRLAERVGTELEIPVYLYEAAASRPERRNLAAIRSGEYEA
                     LPQKLADPEWAPDFGPTQFNSRSGATVIGARDFLVAYNANLNTTSVRRANSVAFDVRE
                     QGRVLRDGDPVTGPVRTDENGEPMRIPGACKAVKAIGWYIEEYGIAQVSMNLTD"
     assembly_gap    20900..20909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20910..21220)
                     /locus_tag="PK198_02655"
     CDS             complement(<20910..21220)
                     /locus_tag="PK198_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRF37664.1"
                     /translation="MKQIIECVPNFSEGRNPEVISQITAVIEQTEGVQLLDVDPGKAT
                     HRTVVTFAGEPAAVVEAAFRAIREAARLIDMAAHTGEHPRMGATDVCPLVPISGISLE
                     ET"
     gene            complement(21222..>21747)
                     /locus_tag="PK198_02660"
     CDS             complement(21222..>21747)
                     /locus_tag="PK198_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37665.1"
                     /translation="NPKPRAFPKIIYPDRNFVGFTQNACDFSFEYPDYMSIEKDSSFF
                     DEAPPHPCWFNLRFPQFNGTLHCSYAPINSRQSLEKLNVDMFEMTDWHNKKATYIDTK
                     VIKLPNGTSGFVFNIEGPVASPFQFYVTDSTQHFMRGALYFDAAVNADSIAPVIDFVR
                     KDIMHIIETFQWSK"
     assembly_gap    21748..21793
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21822..>22138)
                     /locus_tag="PK198_02665"
     CDS             complement(21822..>22138)
                     /locus_tag="PK198_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRF37666.1"
                     /translation="IIGDIKDEFDDETDVVYQKLDDYNYLFEGKTLLNDLCRIIGVPV
                     EAFDEVKGEADSLAGLLLELCGQFPRKDAQLQHNGFTFKIVSVTKRRIEQVRVTIHKS
                     EA"
     assembly_gap    22139..22178
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22179..23238)
                     /locus_tag="PK198_02670"
     CDS             complement(<22179..23238)
                     /locus_tag="PK198_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRF37667.1"
                     /translation="METESEIPLYLYQLLLSAPVMWQAWLAGVLLLLICSGLISGSEV
                     AFFSLSHNDFEVLSEERSGVAQRILHLKDHPRLLLATILISNNFVNISIVILSEFILR
                     SWLPEAQIEHWAQGITSWMGYGVEAASQWADVLSFLITVVGVTFLLVLFGEVAPKVYA
                     TQNNLQLARIMSSPLSTLSVLFKPLSFLLMRSTGLIEKRLAHRVKTSSQASKEDIGEA
                     IDLTVSREHQTGSRELDILHSIVKFSGVSVRQIMTPRLDIVGVENTASYEELLNIARE
                     SGYSRIPVFEEDLDQVVGLLYVKDLLGHLKDGDFDWQSVIRTNVMYVPESKKIDELLR
                     EFQKEHLHLAIVVDEYGGT"
     gene            complement(23284..23721)
                     /gene="ssb"
                     /locus_tag="PK198_02675"
     CDS             complement(23284..23721)
                     /gene="ssb"
                     /locus_tag="PK198_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRF37668.1"
                     /translation="MVNRVILIGNLGKDPEVRRLENGAVVAKFTLATNESYKDREGNW
                     QTTTEWHEIVVWRQLAERAESSLKKGMSVYVEGKLTHRTWQDQDGNSRRTTEVVANYF
                     RSLGKREDAYTPQPQVATVEGAAPMPDQNDVNSNDAPADDLPF"
     assembly_gap    23997..24034
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24035..>24348)
                     /locus_tag="PK198_02680"
     CDS             complement(<24035..>24348)
                     /locus_tag="PK198_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37669.1"
                     /translation="PLCRQCPVSEHCRALAEGTVSDLPLKSKSIVKRERFFHYLVVEQ
                     AGKVLISRREGKDIWNGLYEFPLLELPALTTALDVLQSDAGWNEILQNTDALVASVSP
                     PF"
BASE COUNT         5504 a         6693 c         6011 g         5467 t
ORIGIN      
        1 acaacggggc caacaacctg aacatcacgg cagtagctgt gccgggtgtg tgcggcgcgc
       61 cgggccagat tcatgtgggc attaccggcg gaacggctcc gtttaccgtg agttggaccg
      121 gcccgcaaaa cggttcggcc tctacgagca acaatttcta caatatcctg aatctgacga
      181 gcggtactta caccgtgacc gtaaccgacg ccaacggatg tacgcgcacc cagaccgtca
      241 ttctcaacaa cggggccaac aacctgcaag tgacgctcac ccccgtgccc ggcatttgtg
      301 ttcaaacagg tcagatacag gtaggaattg ccggcggcac agcgccttat accgtatcct
      361 ggaccggccc gcaaaacggc atggccacca caaacacagg ttcttacacc ataatgaacc
      421 tgccgagcgg ggcttacacc gtgaccgtaa cggacgccaa cggatgtact gccacccaaa
      481 cgacgacgct gaacaacggc ggcatcgtga cgctgacagc cgcgtctgcc aatggaatct
      541 gcggccagaa cggttcgata caattgacga tgaccggcgg ggctgcgcct tatgccctga
      601 gctggaccgg acctgtttcc ggtaacgcca cggccaacgg aggcgcttac accatctcca
      661 acctgccctc aggtacttat attattacgg caacttcggg cgccaattgc gtaagcacac
      721 aaaccagcac cctaaacaac ggctctgtct tcaacatgac ggtcgtttcc aatccgggct
      781 tctgcaatat caacggctct gtagtgttga ccttcagtga aggacaaccg ccgattgcca
      841 tcacctggag cggcccggtg aatggttctg ccaccacttc gggcaacagc tatacgctct
      901 ccaatttgcc cacaggcact tacaccatta cggcaacgac ggcacaggga tgcacagaca
      961 cggaataacg ccacgaatat gttgacggtg acaggaactc cggtgaacgg cttgtgcggc
     1021 gaaccgggtt ccgtaaccct ctcaatgaca ggcggtaccg ggccttatac catcgcctgg
     1081 acgggagcaa gctccggctc taccacctcg gccaatgctt tggtaacgat tcccaatctg
     1141 ggaaccggca cctacaattt cacggtcacc tctgccaacg gttgcgtcca atccacgtct
     1201 gcgtctgtgc aggtaactga atcagacata gacattacca gcacggccaa taccggaaac
     1261 tgtggtgaat ccggttccat tcaggtgatt atcagcggag gcaatccggg gtatgtcatc
     1321 aacnnnnnnn nnnnnnnnnn nnnnnnnnnt accacttccg gcagcactta cgtcatcaac
     1381 ggcacaacag ccggcgatta caacatttca gtaaccgacg ctcaggggtg tacagaaaca
     1441 acttctgtaa cgctgaacaa cccggaagcc gatctggaca tcctgttgag taggatgaac
     1501 ggcatctgta gcggccccgg cgccgtgaca gtcagtatca tcagcggaga tgctccattc
     1561 aacattgtct ggaccggccc cgtcaatggt tctaccacta cttccaacag cagtttcacc
     1621 atcaacaatg ctccggcagg tacttacacc gtaaccgtga cggatgtaga tggctgtacg
     1681 gatacaggaa ccatcaccgt cacaaccacg ccgaatgtag tagccactac cattgctcaa
     1741 aacggtgtgt gcggcatgcc cggctccatt attctgaatc tggtgggcgg cacagctcct
     1801 tacaccattg cctggacggg cgctgcaagt ggttctgtca cctcgcaaac caccacgcgc
     1861 acgttcaaca atctgggtag cggcacatac aacttcaccg taacggatgc caacggctgc
     1921 gttgcaacga ccactgcggt gctgaacaac agcggcaaca tcttcaccat caacgctttc
     1981 ccgtatcatg gcacctgcgg agatttgggc cgcatacgcg tgaacatgtt cggcggcacc
     2041 gggccgttcc tcatcatctg ggtggggccg tcttccggtt cggcaacgac ttctgcgacc
     2101 acttatgaca ttttgaacct gcccagcggc atgtatttcg tcagcgtgac ggacgcggcc
     2161 ggctgtacgg gtaccgacgt ggagatgatt accaactcca acacctcgtc catcaacgtt
     2221 tcggtgacgg ccatcaacgg gctgtgtgga gatttgggtt ccatctggat tacggtgttg
     2281 caaggccaag gaccgtttgt catcacctgg accgggccgc aatcggggca gatcacgact
     2341 tcttctaccg atttcgatat tcccaatttg cccaacggca cttacaccgt taccatccgg
     2401 catgtgaatt tgtgtgaaag aacctatacg gttgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccccggctc cattacggtg aatattgcag
     2521 gcaatgcgcc gccgtacatc atcaactgga acagcgccat cggcagcggc acgcaaacgg
     2581 tcagtaccaa caccttcacc attcccaacc tcttcaccgg caattacacc gtgcaggtag
     2641 tggatgccaa tggctgttcg gaaacgcata cgagacagtt gtacaacgag gcgggtacct
     2701 tcattatgac gctgacgccg aatgtgggcg ggtgcaacaa ccaaagctct attcaggcca
     2761 gcatttccgg tggacaggcg cccttcctcc tcaactggac cggcccaagt tccaatggtt
     2821 cggtaacctt tacaacctct acctacaacc tcttcggcct cgcgccgggc ttctatatga
     2881 ttatgattac ggatgccaac ggttgttcgc agtgggaaac agtgcaggtg tcttatgcgt
     2941 cttccccgcc gattacagcg tttgcattta ccaccaacct gctgacggcc aacttcacca
     3001 accaatcctc accgggtact tacctgtgga gttttggaga cggcacaacg tccacagcgg
     3061 ccaatccggt gcatacctac gcacaggacg gcacctacac ggtatgcctc attgtgagca
     3121 acgcctgcgg cagcacaacg cactgcgcca acgtaacggt gacgattccg aacaacatgg
     3181 tcattttaga cattggcgag aacagcgggg ctgcgggcgg caacgtctta gtacctgtgc
     3241 gcatccgcaa ctgcaacctg ctggtgtcgc tggccggctc gctgacggtg cagaatcctt
     3301 cagtggcaag cattgcagga gtggcgccgg ctgccattgc gccgcagttc aatccggtca
     3361 acaagacctt caattactac gccaacaatg gtcagggcgt gagtttgcag caaaatcaga
     3421 tactcttcta tttgattgtt cagcttaccg gcacgccggg gcagtccacc aatttgagtt
     3481 ttgccaattc gccgttagcc gtagaagtgg gcagtatggt gaacggtaac nnnnnnnnnn
     3541 nnnnnnnatc acatacacgg gcagtgtttc catctccaca gccgtacagg tttccggcga
     3601 cgtttatacc tattgggacg ccccgatccc ggatgctgaa gttcgcatca gcaataccgg
     3661 tatggacatg atggaaatga ccgatgatgc aggccgctat atggcgccgg atgttccgat
     3721 gggcgagatg attaccatta cgccctccaa aaacggccat ccggccaacg ggctgagtac
     3781 ctacgctctg ttcatcgggc agcgctttat actcggaatg gagccgccac aagttgcatc
     3841 cccgtatcaa gtgattgccg gcgatgccaa ctgcaacggc gcgttcacca cgctcgattt
     3901 gttcatnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcgt gcgcgacagc tttgccaact
     3961 gcccggcctg ggtattcgtt gccgcaaatt ccaacttgcc ggaaagcgat ttcgatgcct
     4021 acaacgtttt cccctacgcc aattgcgaca gcatgatggt gacgggcgac gcgctggctg
     4081 atttcgtcgg tgtgaaagtg ggcgatattt tgggccatgc caatccggcc aacttcacca
     4141 acggcggcgg cattgaaccg cgcagcgcag ccgaattggt attgactacc aacaaccggc
     4201 aggcacaaca gggcgatttg atcagtattc cggtgcgcag ttccaacttt gaggacatta
     4261 ccaactacca actgggactg tggttcgata cccaatacct cacttttgaa ggatttgacc
     4321 cggcagcttc cggagcatta tcttctgtgg tggcgggtac gactcaaacc ggcaacggca
     4381 ggctcagcgt gagttggttc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagagcgta aaggcatcat
     4561 tctgaatatt gaaaatcagg ctgttaccag cgccaacgag ccattggaag gcaaaggcac
     4621 tttccgtttg caccagaacg cgcccaaccc gtttgtggct gaaacgtcca tctcattcga
     4681 tcttcccgaa gcatcggatg tggaattgct catcaccgat cagttgggac gcaccgttcg
     4741 catactgcgc ggccaatacc cgcaagggcg cagcacgatg gatttcaaag cggaagaact
     4801 gccgcagggc gtctatcgct acacgctcaa agcgggcgag ttctcggcaa cccgaacgat
     4861 gattttagtg aaataacagc atacggagag cggcttcaaa ataaggggcc gctctctttg
     4921 tttttaatct caaaaaccat gatccaccta ttgcgtcggt gctgcctgtt gttggcagta
     4981 tggcaggcgc ctttcttccc gtctgcccaa accatccaac tctccatgcc ggataccacc
     5041 gttttggccg gcgacaccct ctcctacccc atcactgcaa cggcaggctt caacggcgta
     5101 gtcagcgcgc agttttccat acaatggaat ccctccgtac tcagatacat cgggcacctt
     5161 ccggttgatc ttaatcagat tgccgtgggt gaaacgagcg ctgcacaggg cgccttgagg
     5221 ttctcctggt ttgacgtggg cgggcagggc attacgctgg cgcccggtag ccagttggtg
     5281 cgatttcgtt ttcgggccga ggggcagccc ggagattcta ccccggtagt ctttactaat
     5341 gaaccgctgc gcattcagat attccgggcg ggcagcacgc cgggcttgtt cgattcgctc
     5401 gttttggtgc aagataccgg cttggtgaag atcgcccgcg tttggcagcc gacagccgtc
     5461 tcacaaaacc ccacttgttt cggaatgaac gacggcacgg cctccgtcaa tgccgcagat
     5521 gctccggcag gcgcgcaatt cctctggacc gggccaaacg ggttcagctc ttcgtcgcca
     5581 gtattgagtg acctcggccc cggcgcttac aacttggtgg caacatcctc tcaaggcgag
     5641 gttttatttg aaacgaccat caccctgaac gagccaccgc ctctgcaacc cgatctgggg
     5701 gacgacctcc agctttgtcc cggagaaagc tcgacgttgn nnnnnnnnnn nnnnnnnnnn
     5761 atttgtggtc cgacggaact accgctgcgc aattgcccat caaccagacg ggaacgtaca
     5821 gcgtaaccgt gaacgacgcg cagggctgca cggcttccga caccattgca gtaaatgttt
     5881 ccgctgatgt tcagatagtt gtagaaaacg accggctcag catctgcccc ggcgacagcg
     5941 tgcaattgcg cgtttcgggc ggggaaacct accgctggac cgacccttcc aacaccctca
     6001 gcagcaccac cattcccaac cccgtagcca agccgcaaca gagcagttcc tacaccctca
     6061 tcgcctccaa tgaatgtgca tccgactcca tacaattctc catttttgta caccagacag
     6121 atgcctcggc aggccccgac acttgcattc agcgcggcca ggagctgcgc ctgtacgctc
     6181 gcggcggagt gcgctatgcc tgggatcgcg gagagtaccc tctgaacagt tacagcagtt
     6241 ccgacccgct cagcacgccc ttgagcagca ctgtttacag ggtggaaatt gtagatgcca
     6301 atggttgtaa aaccaccatt gaggtgcccg taacggtggt ggacggcgcg cccgacatac
     6361 tgctcatcaa catgataacg cccaacggag acggcagaaa cgacgtgctg gagtttcagg
     6421 aattggggaa gtttaccaac aatgccctca aggtgtataa tcgctggggc gatttggtgt
     6481 atcagaaatt gggctaccaa accgacgatg agcgatttga tggcacttgg aacggcaaac
     6541 cgctgccggc gggcaattat ttttacgtcc tgtctttttc agaagtgcat tacaaacaaa
     6601 ccctcaccat cataagagac tgacatgaaa cacattgcat acatattctt cctctcgttg
     6661 tggccctttt tcctctgcgc ccaacaactt cccgaccgca gtatttttgc agaaacgaat
     6721 ttcctgtgga acccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     6781 caagctaccg gcagcaatgg ttaggcttcg acaatgctcc ccgaactgcg gcggtggcaa
     6841 ttcaataccc cttgccggaa tcgaactcgg cgctgggcgg ctattttatg tacgatcaga
     6901 tcaatccatt ggtagtcaac cacttcacat ttcagtacgc ttacaaactc cgctttggcg
     6961 accgcaaacg ccggcagctt tccctcggca ttgcggccaa tgtgcagcat tttctgctcg
     7021 atgcccgcga tgtggtggtc aaccacgccg atgacccgct gctgcctgtg ggcgaataca
     7081 acaccgtagg ccccaacgcg tcgggcggca tcttttacac ctccgatgcc cggcaaagcg
     7141 acaacaaagg cgcttggtat gcgggcgcag ccatacagca ggccgtgccc tggaatgtag
     7201 tttttgacga caacgacagt ccggccaact tgcggcatac ctggcacgga aatgccattt
     7261 tcggagggcg ttttgccaaa ggcggcgtca ttctggagcc tgccctgtgg gtacactacg
     7321 cacagcccgg cattgtaaac ggcgcttttc acttgcgtgc cgaaagccgg gatgcgctgt
     7381 ggggcgggct gagttattcg tccaaccaaa cgctgggcat acaggccggc gtagtagtta
     7441 aaaaaggatt taaagaattc agccacctgc gcatcggcgt ggcaggcacg ttcaatattg
     7501 gtacattcgg acagtttcgc gggccgggct acgagttctt gatggcttac cggatggcgc
     7561 aggactgagt ggtgtgaagt tatcctttct tagccggagc gcgtaaattc tgctccgact
     7621 tctcgttttg caccgaaata gtgacctgtg cgtacctccg gcgggcatcc cgaagccaat
     7681 cggctgcttc cttgccgcga atgccgccat cctgtgcgcc gtcgcggatg atacgcaatt
     7741 ggccgctttt caggtaggat tgcagcgccc cccttccata attcgacatc cagatatata
     7801 ccgactctgc ccgacgagca gccaactgcg aggcatcccg ctcctgatcg cctgcaaaac
     7861 cactgatgcg taccacgatg gattcgcccg gcgccagccc ggttttcagc ttggagtcta
     7921 tttccgcagt agcctgcgta agggtttcaa aagcgttgga agcctgattg aaaaagcctg
     7981 cgatgtcatc ggttgccgag acgttgtttt gctgcactac attggagaag ttacgccgca
     8041 gttcatcgcg ccgggcaagg taagctttca gcaagtcttc atagcttgtc gccgttttct
     8101 ctggaatatt ttcatcaaaa taaagcgaca tgggcaaggt gtaattcaat accgtaggcg
     8161 tattgggcac aggcgtctga ttcccttgcg ggcaattggc cacacattta ttggatttgt
     8221 ctatgacgaa cgtactcgtt tcgcctttag agttcagcga aacctcggcc cgcccccctg
     8281 aaaaatcgcc cgcacggagg tattgaaacg gaatcacggt ttggcctgcc atattgatgt
     8341 atccccagcc gctctccatc tcggcgcgaa taagcccttc ccgcagcggc ccgatgttcc
     8401 tgtaccgaat gggaatggcc acactgcctg cactgttcaa atatccaaaa atggaacttc
     8461 cccgcaggcg ctgttccacc ttgtaccagt ttccggccgc gtcatagtca atggagtagt
     8521 actcggcagg cgccagcaca ttgcctttgg cgtcttgtag tcccaccaat tctccttttc
     8581 tgaaggtgcg cagtcgttca tctttcactg gagcctcctg cgtcatttcc accctcggag
     8641 gcgtggcgcc gggttgcagc ttcaccgtca gttgggcggg cgcgtagcct tggcgggtaa
     8701 actgcaaagt caccaagctg ccctctgttg cagccggaaa cgacagggtg tatgcccccg
     8761 ttttgtcgga ataatccgta aatgatccgg cagatacttt taccttagcc agcggccgct
     8821 tggtagccgc ggcctgcacg atgcctcgca ccacaatagg tttcagttcg ccatcgggat
     8881 cgggcagcgc tgttgccggt tcatcctgct ttttgcagcg caccgccgcc gacagaatat
     8941 cggtatccaa catgcccctg gcggcaattt ccgactcata agcctttgca aaccaaaaac
     9001 atgcagattc cggccacggg tcgccggcaa caggctggta gccggcatct atctgaagac
     9061 gggaaaattc ctgcaccccc tcattgtata aatacactgc aacatttttg agaaaagcaa
     9121 tggtatccgc agaaatacaa gctggaaaca gcgcacgact cgcctctatc agactgtctg
     9181 tggcggccat atttttttgt tggatcaaat cgccgaccaa catttcattg taatacaact
     9241 gatctgtagg gttgtgaata caaagcgccc tgttttgata aacaacagat tcgccgtctg
     9301 cgcacacgtc aaacggcggg ttgtaccacc aggcaaaagc ggtgaaaaat gcccacagcg
     9361 gaatggccca gagcaagtca aaggcggcag gtcgaaggcg gtcggcggca ggttcggcca
     9421 atttcctcag ccagggcgga atggtgaatg ccgttcggga aggcggttcc tctacatatt
     9481 tgaaagccac ctcgtccacc ggatgcccgg cagtgtgcaa gccctgcaac tcagccttga
     9541 gcagattctt ttctttacta ttagaagtaa tggccaaggc attgagcgcc gcattgaaac
     9601 ggtactcttc ataagcagca gatgccacat cggggggcgt gctttgctgc aagaggttgt
     9661 gaatgatgcc gcgcacccgc tcctccatgc ttttggaagc caaatactct accaattctg
     9721 tgcgggccgc ttccgggatg acgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     9781 ggagctgatc gaaatgcagc aaggtagcgc ccggcggcga aagttgtctg cccagcgcca
     9841 gagtcagttc ccattgcaag agcggataca cggcgcaggc cgcgatccat tcaatcatcg
     9901 gctccgaaaa gtgcttccgc aaagaagtca tcagatcacc ctccagcaca atcggctcgc
     9961 gctgaatgtc ttccagacgg gccagccact ctgaagtatg gataggctct gtggcgtcca
    10021 actgttctac ggcggcgctc cagccctgca aagacatggg caacagataa aacacctcct
    10081 cgatgcgctg ctcccggcgg ccccacacac tcacgggaat aggcgacaac atggcccggc
    10141 ggcgccagtc ggtgagcata gacacccagc gttcgagcct gccggtgagc gggctgagga
    10201 gttggtgccc atgcccgatg acgatgagcc gggcattgcc gtagcgatgt tgcagctcgc
    10261 gcagtgggat gccttcgggg ttcttttcat caaaacagag ccggatatcg ccctcaaaaa
    10321 aatagcgggc taagggcgct tcctgcgttt ggaaatacct gcaaacccag tcgtagagcg
    10381 ccgaccggtg atcgttaggg ctttggcggt caatcagcag gaggtagtcg ggcggattgg
    10441 tgagtcgttt gtaccgaaaa tcggccatgc ccagccggtt gatggtagcg cggatggagg
    10501 ccggaatgtc caactgcata gcatcgcccc ggatgcggcg gcgcaggagg aagagcaatt
    10561 gggcggcaga atcgccgggc acgatggcgc cgggctgctc tatgcgaaac ctgaaatgat
    10621 agggcgggcc gctgcggcct ttttgctggg tgacggccgt gaggaacctg cgccggcgtt
    10681 cgcgccattg caacagcgcc aacaaaccta atgcggaaag aacggtaagc gccgatttga
    10741 ccacccaggc gtattgcgta tataaatgaa tggcatactc ccgacccgga tcgggcagca
    10801 gttcgcgcat gtctctggga aagggaacat tttttgtaga aggcaatgcc aggctcgtat
    10861 cgggtttcac caagtcgagg tcttcaatgc ccaattggag gctaatgaca aaaaatgttg
    10921 tatcgcggcg cgccgaggcc aaccacgcct ctactaaaat ggtatctctg aaaaaatcgt
    10981 aggtatctct gacgatgtaa gtaaacaagc cctgcggact gacctgatac gctccgaaaa
    11041 ccgaatctga gccggaagaa gcgccgttgc cgagtaaggc catcagcagc gaatcgccct
    11101 gcggaatgga gatgcgttcg gtcagcgtat cgccggggcg caagacgcgt tccacatatt
    11161 ggggcaacag cggcggcggg ggtttcgggc cgacctgttg tttcaccgcc acgcccgcca
    11221 caatggctaa gatttgcgcc agcaaccacc atttgcgctt gcgcggcgac gccaccgtgc
    11281 ggcgtacaaa atacccggca gccaacagcg aaagtacaac gatgggtatg gtgggattgc
    11341 gccaggaact gaatacctcc acatccaaca aaaagccgcc cagaaaacta aaacccgccg
    11401 tcatcaacag caggagattg cgccaaaccc gcgccagttg tcgctctctg gctgaatcag
    11461 accgttcggc ggatgcctcc gacaacagca caacccgctg ccaaacctgc tcgaacagtc
    11521 ggtaaaaatc ttcctgttcc tgccgattgc gggcaaacaa aggacagagc aaagccgaca
    11581 gccgttcggg cgctatatcg gcaggcaggc gccgcagcaa ttcctgaacc tgaaggtgct
    11641 tgccggtgcc catgtcgtag ccgtcggctt ccagcgcggc cgtcagttca gacatcagta
    11701 tgtggaggcg ggtatcggtc attgcagaca aaatactgat tgacaattta ttacagaggc
    11761 gtcattcatg cactgaatct caattgattg cgtactctct gcttttccgt ttgccgcatt
    11821 tcgtcatccg ttctgacatt ctcctccatt cttttggcca gcagcttcca ccagtacacg
    11881 gcaggaatga tgtttccaca gtgggtttcc aacctgtaag tgagttctac gttgggattt
    11941 tcccggtcgt tcagcagccg gctcagcttg gtaggatgca ggtcaacatc ttcggcaaac
    12001 tccttatggc ttcggcctaa tattttgatg tattgttcca attgtttgga gaacgaaaac
    12061 gcctcttcaa aaccgcttcg ttcaaagtag tcccgcatca acaacttcat ccgcatcagc
    12121 tcagagagca ggcgctgctc ttctgttctt tccaataacg ctttggctct cagctttcta
    12181 aactcttcct cgatttcggt ttgttcattt ttttccagat cgcctgaaaa gatatacgcg
    12241 ccggcaattt cctcaacggt aaactcctca agcaaatgtt catagatggt gtcgtattta
    12301 ttcatcgcca atgtatttat caatgagttg tttggatgat tcgtattcta cggccaaaag
    12361 tcgcccatat tgtcgaaatc catatttgtc ctgatagtgg tggatgagtc tggttttcgg
    12421 caataaagaa acaaagccgc catagcccct tttaaacgca gatgcacagg caaaaccgat
    12481 gaggcaacca gcaatattat ccaaggtttt ctccttacct acctgagttt tggttatttc
    12541 tatcaggttg atgtgaactc gcagctcctc tggatagtcc tttaaagaaa gcaggcccag
    12601 aatctcattt tcatcctctt tgagataaag tttgtaaacc tcatgcccca attcctgctc
    12661 ccagtcaaag ctgaaccccg gatgctttcg tattttagca atgtctttgc ttgtcgcttc
    12721 ctctatgaag acctcaacca aactgccgga tgctttccga gttactttca taatggtcta
    12781 ttctgttcgg ccggcagggt tgtttcattc cgccgttaca aaaatacaaa tttctgttta
    12841 cattttacat aaactttcat tttttttgtc taaaaacaat ctattacctt tgttccaaaa
    12901 ccaacaatat gaaatatctc gcttctttat gcctgctgat cgtgatgtca actgctcagg
    12961 cgcaacacca cccactgctt caatccggtc cgatgcccgg ctacaccgac atgctcgaaa
    13021 ccacgctctg ggtacaaacc aaagcgcccg ccaaagtaca cattgcctac tggaatgccg
    13081 caggcaaagc cgataccctg ctcaccgagc cggttacaac gcaaaaacac agtgcctata
    13141 ccgccaaact cgtggccgac aaagtgcagc ccggccaaaa gtacaactac cagctccgca
    13201 tagacggaaa agcagtgtcg ctcccctacc ccactcaatt ccagacactt ccgctgtggc
    13261 aatggcgcgc cgacccgccc aattttacac tggcaaccgg aagttgcaac tacgtcaatg
    13321 agcctgacta cgaccggccc gggcaaggct acggcagcga tcatcatatt tttaaagcca
    13381 tccacgaccg gcgacccgat gtcatgctct ggctcggcga caatacctac ctccgcgaac
    13441 ccgactggaa cacccgcacc ggcatcctgt accgaaacac acacagccgt tctctgccgg
    13501 aactacagcc cctgctcgcc tccacgcacc actacgccat ctgggacgat cacgatttcg
    13561 gtcccaacga ctccgacggc agcttcccat tcaaagacct gacctacgaa gccttccaac
    13621 tcttctggcc caatccctcc tttggcgtca acggtcaaaa aggcattacc acctttttcc
    13681 aatgggccga cattgacttc ttcctcttag acaaccggta tttccgcacc gccaacaacc
    13741 gcaaaaccgg cgacatgcaa atactgggcg ccgaacaaat tgactggctc atcagcgctt
    13801 tgagcaacag ccgctccccc ttcaaaatag tagccatcgg cgggcaggta ctcaacacgg
    13861 cccccgtaca tgaaaactac atccacttcc atgccgccga acgggaattc ctcctcaaac
    13921 gcattgaaga ggaaaaaatc aaaggggtca tcttcctcac cggcgaccgc caccataccg
    13981 aaatgagcaa attggtcaac aaagccggca ataccgttta cgacctcacc acctcgcccc
    14041 tcaccgccgg cactgcccgc gcaagtgaag atgaaccaaa tatttatcgg attcccggca
    14101 caatggtggt aggaaaacac aacttttcca cactggagtt ttccggccct cgcaccgccc
    14161 gcgtactcac catccgcgta ttcgatgcag agggcgccca attgtggcaa cgcgaaataa
    14221 aagcaggtga atagcgcttt ttttttgcat tttttttctt ggcagattga ataaatagcg
    14281 ccaattttga tgcgtcgttt caggcagacc ttatgcacag aacagcttcg gtccatattc
    14341 tttgcaatga attgaataac aatgaaaaag caaatcgctc tgttgtctta cttcctcatc
    14401 gcgcagatac tgcccctcgc agcccaatct gccgataccg actttatgcg cagcaggggc
    14461 aaaatctttg tcgttgtggc ggtcattgcg gcggtattca tcggaattgt tctctttatg
    14521 gtatttctgg aacggcggct aaccaaatta gagaaccaaa tcaacgaaca tgagccaaca
    14581 gaccgtcgtt agcttttacg acagcgtaac caactttttc aataaagccg cccaacatac
    14641 cgggcttccc gaaggcttat tggaacaaat ccgcgtttgc aacgccgttt accgcatgct
    14701 ctttcctgta aaaatgcagg acggacaggt aaaagtgatc gaagcatacc gcgtacagca
    14761 cagccaccac cgtacgccta ccaaaggggg gattcgctac tccaaccatg tcaaccagga
    14821 agaagtaatg gccctcgcca ctctgatgac ttacaaatgc gccattgtgg acgtgccctt
    14881 cggtggcgca aaaggcggtg taaaaatcaa cccctgggac tactccccgg aagtgttgga
    14941 gcgcatcact cgccgctaca ccaccgaatt gatgagccgc aacttcatcg gcccatctat
    15001 tgacgttccc gcccccgact acggcaccgg cgaacgcgaa atggcatgga tttacgacac
    15061 ctaccgcact tttaaaggcg ccgatataga cgcagcgggc gttgtcacag gtaaacccgt
    15121 cacgcagcac ggcatcaacg gacgccgcga agctaccggc cgcggggtat attacggtat
    15181 ccgcgaagcc tgcaacatac cggaagacat gaagcgtttg aacatgacta ccggcctgaa
    15241 aggcaagcgc ctcatcattc agggtctcgg caatgtaggc tccttctccg gcaccatttc
    15301 tcaggatgaa ggcggcgcca tcatcgtggg cgtaggcgaa atggaaggcg ctatttacaa
    15361 caaagacgga tttgacatca atgatgtact gaagttccgc aaagaaaccg gttccatcct
    15421 caactatccc ggcgcgattt ctatcccgaa agagcaacgc gaaaccatct tggaatacga
    15481 ttgcgacatt ctggtacccg ctgcattgga agacgtcatc tcttataaaa acgctcccaa
    15541 tatcaaggcc aaaatcgttg cggaagcagc caatggcccc atcacttccc gcggcgagga
    15601 aatgcttttg gaagctggta aaatcatcat ccccgacgtg ttcctcaatg ccggcggcgt
    15661 gacggtttct tacttcgagt ggttgaaaaa cttgtcgcac atgcgcttcg gacgtttgga
    15721 aaaacgcttc gatcagcagc gcctcgaaaa cctcgtgggc ttggttgagc gcaccgtggg
    15781 ccgtgagatc agcgacgaag aaaaacgcat gattgcccgc ggcgccgatg agattgacct
    15841 ggttcgctcc ggcctcgaag aaaccatgat caacgcttat caccagatca gagatgttat
    15901 ggttgataaa cccaaagtaa ccgacctgag aacggcggcc tttgtgagcg ccatcaacaa
    15961 gatcagcagc gactacatgt cgctcggcat tttcccctga ttttcagggc tttgttttca
    16021 aaactacaaa ccgtcttctg ccgctcgcag gaggcggttt gtttttttag gactgcccct
    16081 ttacacctga aacactccca cgttgaactt ctctaccggc gcgttgtcgg ccgcttcgat
    16141 gctcatggaa atggctctgc gggtatcggc ggggttgatg atttcatcca cccagaggcg
    16201 ggcagccgcg tagtacggcg tggtttggcg ctcgtacttg gacttgatgt cgtggagcaa
    16261 atgcgcttcg tcttcgggcg agagttgttg ccacttggct ttgaggcctg ctgtttgaat
    16321 ctggagcaaa gtcttggctg cctgctcgcc tcccatgacg gcaattttgg cgttgggcca
    16381 ggcaaaaatg aatcggggat cgtaggcttt gccgcacatg gcatagttgc ccgcaccgta
    16441 agaattacct acaacaatgc tgatcttcgg cacggtagaa ttggccactg cattcaccat
    16501 cttcgccccg tctttgatga tgccgccgtg ctcggagcgc gagcctacca tgaatccggt
    16561 gacatcgtgc aggaaaacca gcggaatttt tttctggttg cagttcataa taaaccgggc
    16621 cgccttatcg gcagaatcgg aataaatgac accgcccacc tgcatttcgc ctttgggcgt
    16681 gcgcaccacc tcgcggttgt tggccacgat gcccaccgac cagccgtcaa tgcgggcata
    16741 ggcgcagatg atggttttgc cgtagtcttt tttataaaaa gtcagttcgg aatcgtccac
    16801 tattcggcgc agcagttccg tcatgctgta cggtttggcg ctgttttcgg gtacgatacc
    16861 gaatatttca gacggatttt ctttgggcag gcggggttca gcgcggtcga aaccggcctt
    16921 gggaaacgtg ccgagtttgt ccaccagatc gcggatggta ctcagacagg ttgggtcgtc
    16981 gggcatgcgg taatcggtga tgccggaaat ttcggcagtg gtacgcgcgc cgccaagtgt
    17041 ttctttgtca atatcttctc cgatagcggc tttcaccaaa taaggtcccg ctaaaaaaat
    17101 agaaccggtg ccgtccacaa tcaatgcctc atccgacatg atgggcaggt aggcgccgcc
    17161 tgccacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnggaaa atgcggccaa agtgctcttt gtcggggaat
    17281 atttcatcct gcataggcag gtaaacgccc gcgctgtcca ccaaatagat gatgggcagg
    17341 cggttttcca tggcaatttc ctgcgcccgc aggttctttt tccctgtaat gggaaaccag
    17401 gcgccggcct tcacggtggc gtcattggca acgataacac attgacggcc acgcacataa
    17461 cctatgccta ccacaactcc gccggcaggg caaccaccat gctcctcgta catttcgaaa
    17521 ccggcaaaag cgccgatttc cataaaataa gaatctggat caatgagatg atttactcgt
    17581 tctcgggccg tgagcttgcc caaactgtgt tctttttcaa ttttagcctt gccgccgccc
    17641 tgatgaattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn ntcttctttg gtcattgtgg ttgccgtttg taaagaaggg
    17761 ttaaccgcgc gtcaaccctt cttcaaatat tcaattctta tttacctttt tgcgctccaa
    17821 ttcgatgacc gtaccttttt tcatacgttc ctcattctct tgttgacgct tttccttaat
    17881 cgcctcaatc tgaactttga tctccaaagg ctttttgttg agatagcgat gataggacac
    17941 gatgacgatc tgcgacatgt cttcagggta gcttactccc agcgatttga ggtagtgaga
    18001 aaatcttgac ccctcataaa atccccattt ttgggaaatc cagcggccca aactgaagaa
    18061 caatttatgc accacttcgt cttccgtagc cgatttgaag gtagctttgg cagaaggctc
    18121 aatcaaacga ttcagttctt cgaaggcatt caccaaatct ttgggtatgt aaatgccgtc
    18181 caagcgctcc tgcttgatgc gccattgata attcttttca aattcggcct cggtttcagc
    18241 aggcttggcc agttgagcaa atgccggcat tgttgccgca aacagcagca acaggagagc
    18301 aaacaggttt tttccagaca taatgaatcg taatgttgtg aatgtatcga gtgatgaaac
    18361 aacgccacct cccgatacac tcatcgcgag gcggactacc agcgaatcag cgccgagccc
    18421 caggtaaagc cgctgccgaa tgccgccaga caaactaaat cgcccgcctt gacggccccg
    18481 gcttccaccg cttcgcacaa ggcaatgggg atggaagctg ctgtggtgtt gccataacgt
    18541 tggatattgc tccaaacttg cgcgggcgac aagcctaatt ttttgcctac aaactcgctg
    18601 attctcaggt tggcctgatg aggcaccaac aagttgatat cggcagccgt aagactctga
    18661 ctatgaagtg cttccatgat gacttcaggg aatttttgca ccgccatttt gaacacaaaa
    18721 ggcccgtcca tatacgggta aatctgcccg ttgtcgaaca tgtgctgtgt gatgaacatt
    18781 tggccatact cagtttccgg gtagccccag tcaatgtcct cgcccatttt ttcggagtgg
    18841 tatccgccat gcgcaccggg attaatcatg gccaattttt cggcataggt accattggag
    18901 tgcagtttgg tggtgagtac gcccttgcct tcttcgtcgg tcggttgcaa caccacagcg
    18961 ccggcgccgt ctccgaaaat gaccgtcaca ttgcgacctt cggtagtaaa atccaagccc
    19021 atggagtgaa tctcggcgcc cacgagcagg atgtttttat acatcccggt cttgatgaac
    19081 tgatcggcaa ccgagaggcc atacacgaag ccggagcatt gattgcgaat gtcgagggcg
    19141 ggaatttccg tgtccgtaat gcctaattcg cgctgaagca gcacgccgca gccggggaag
    19201 taataatcgg ggctaagggt ggcaaagatg atgaaatcca cgtctcgttt gctgatgcct
    19261 gcgcgctcaa tggctatttc ggcagcgcgg gcgcccatcg tggtggacgt ttctttgtgt
    19321 ttttcggcac tcgcggatac cagtgcgttc ctgaatccac tcgtcggtag tgtccatcac
    19381 tttctgaaga tcgtggttgg tcactgtttt ttcgggcaca taagagccga tacctgctat
    19441 tttggtgcgg agcataacga tgttgtttat gttggtttta aaaagttggt tcgggtattt
    19501 atcgcttcgc aaatgtaata cttcgtcacg agattttctc agccaccaaa gccataatct
    19561 ctttttccat caaatcggcc tgctccatcc atgctctggc ttgctgcaca gcctgttctg
    19621 caaacgattt gtctttcagc ccgcctgtat tgaccaacac attgaggccc gcgccatgta
    19681 cggctgcacg agcgcacaaa gcgcccactc cggcatctgt caccgagttg gggttgccct
    19741 gctctaccat cgccctcacc ggaccaaagg aagcaagcgc caattccatc gttttcagcg
    19801 gcgtttcaat ggcaaagcgg gtagccgatt gaatggcggc gctgcgggcg gccttttcat
    19861 cgggggtgcc tttcggcaat tggaaggcat tcataatggc attgaaagcg cgcgtgtctt
    19921 cgtccaccaa agccagcagt tgatttttga tgtcctgcgc tttgacggcc caatcggaaa
    19981 actcctccca acgctcgtcc cagccgcgtt tgtgcgagga cagattggct accatggccg
    20041 ccagcgatgc gcccaatgcg cccacataag ccgaaatgga gcctcctccc ggcgcggggc
    20101 tttcagaggc ggtctcatcg gcaaactgta ccaagttttt agcaatcaat gggttggatg
    20161 cttccgactg caatttgtac tcaatcactt tttgtttcgg gtcaaacggc cccaaatcgt
    20221 ccaaacccat tgagcgcact gcaatgcgca agagttcttc ttcggaaacg ccggcagatc
    20281 gcttttgctt gcgcaggaaa taccggcctg catcgagcaa tacctgcaaa ggcaacaagc
    20341 ctacaatttc ggaacccgtg acgcgcattc cccgcgattc ggcgctgcgg caacaagcct
    20401 cgaatgcctn nnnnnnnnnn nnnnnnnnnt gtcggtcagg ttcatcgaaa cctgcgcgat
    20461 gccatattct tcgatgtacc acccgatggc tttcacagct ttgcaggcgc cgggtatgcg
    20521 catcggctcg ccgttttcat cggtgcgaac gggaccggta acggggtcgc cgtcgcggag
    20581 cacgcgcccc tgctcacgca catcgaaggc cacgctgttg gctctgcgca ccgaggtggt
    20641 attgaggttg gcattgtacg ctaccagaaa atcgcgggcg ccaatgacgg tagcgccgct
    20701 gcggctgttg aattgtgtcg ggccaaaatc gggcgcccat tctggatcgg ctagtttttg
    20761 cggcagtgcc tcgtattcgc cagatcggat agccgccaga ttgcggcgct ccgggcggga
    20821 agcggcagcc tcgtagagat acacgggtat ttccagctca gtgcctactc tttctgccag
    20881 gcgcttggcc cactcggccn nnnnnnnnng tttcttccaa actgataccg ctgatgggta
    20941 ccagagggca cacatcggta gcgcccatgc gggggtgttc gccggtgtgc gcagccatgt
    21001 ctatgagccg ggcggcctcc cgaatggctc tgaaagcagc ttctactacg gcggcaggct
    21061 ctccggcaaa agtgactacc gtgcgatgag tggccttgcc ggggtcaacg tccaagagtt
    21121 gaacaccctc agtttgctca ataaccgccg tgatctggct gatgacttcc gggttgcgcc
    21181 cttcgctgaa attgggcaca cattctatga tttgcttcat tttatttcga ccattgaaaa
    21241 gtttcgatga tgtgcatgat gtcttttctg acaaaatcaa taaccggggc aatggagtct
    21301 gcgttcacag ccgcatcaaa atacagtgcg ccccgcatga aatgctgcgt gctgtcggtg
    21361 acgtaaaact gaaacggaga agcgaccggg ccttctatat taaaaacgaa tccgctggtt
    21421 ccgttgggca gtttgatgac tttggtatca atgtaagtgg cttttttgtt gtgccagtcg
    21481 gtcatttcaa acatgtccac attgagtttt tccaatgatt gccggctgtt gatgggcgca
    21541 taactgcaat gcagcgtgcc gttgaactgg ggaaaacgca aattgaacca acaggggtgc
    21601 ggcggagctt cgtcaaagaa ggaagagtct ttttctattg acatgtagtc ggggtattca
    21661 aaagaaaaat cacaggcgtt ttgggtaaac ccgacgaagt ttcgatctgg ataaatgatt
    21721 ttgggaaacg ctctgggttt gggattgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnatcaaca gggcaaaaaa agctgaatat ttcatgcttc agatttatga
    21841 atggttacgc gcacctgttc tattcggcgc ttggtaaccg aaacgatttt gaaagtaaag
    21901 ccattgtgct gaagctgggc gtctttgcgg ggaaactggc cgcagagttc caacagcagg
    21961 ccggctaaag aatctgcttc gcctttgact tcatcaaacg cctctaccgg caccccgatg
    22021 atgcggcaca agtcattgag cagggttttc ccctcgaaaa gatagttgta atcgtcaagt
    22081 ttttgataca ccacatcggt ttcatcatcg aactcgtctt tgatatcgcc gatgatttnn
    22141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gtgccgccgt attcgtccac
    22201 cacgatagcg agatggaggt gttctttctg aaactcccgc aacagctcgt ctattttttt
    22261 cgattcgggc acatacatca cattggtacg aatgaccgat tgccagtcaa aatcgccatc
    22321 tttgaggtgg cccagcaggt ctttgacata caatagcccc acaacctgat cgaggtcttc
    22381 ttcaaaaaca ggaatgcggg agtagccgct ctcccttgct atgttgagga gttcttcata
    22441 actggctgta ttttccaccc ccacaatgtc caatcggggc gtcatgatct ggcgaacaga
    22501 cacgccgctg aacttgacga tgctgtgcag gatatccaat tcgcggctgc ccgtttggtg
    22561 ttcccggctc acggtaaggt ctatggcttc gccgatgtct tctttgctgg cctgagaact
    22621 cgtcttcact ctgtgtgcca atcgcttctc tatcaagccg gtagaacgca tcagcagaaa
    22681 gctcagcggt ttgaacaata ccgacagcgt ggataagggc gatgacatga tgcgcgccag
    22741 ttgcaggttg ttttgagtgg catatacttt gggcgctact tcgccgaaga gcaccaacaa
    22801 aaaagtgacg cccaccaccg tgattaaaaa actcagcacg tcggcccatt gggaagctgc
    22861 ttctactccg taacccatcc acgaggtgat gccttgcgcc caatgttcga tttgagcttc
    22921 gggcaaccaa ctcctcaata tgaattctga gaggatgacg atggagatat tgacgaaatt
    22981 gttgctgatg agaatggtcg ccaacaacag gcggggatgg tctttgaggt gtaaaatgcg
    23041 ttgtgcaacg ccgcttcttt cttccgacag cacctcgaaa tcattgtgcg aaagcgaaaa
    23101 aaatgccacc tccgaaccag agatgagacc cgaacagatc agcagcaaga gcacgccggc
    23161 cagccaagcc tgccacatca ctggcgcaga aagtagaagt tgataaaggt ataagggtat
    23221 ttcagactcg gtttccaatt ggattacagt tggttagaaa aatgtagcga tagcggtctc
    23281 ccatcagaac ggcagatcat cggcaggcgc atcgttgctg ttcacatcgt tttggtcggg
    23341 catgggcgcc gcgccttcca ccgtagccac ctgaggctga ggcgtatagg cgtcttcacg
    23401 cttgccgagg ctcctgaaat aattggccac tacctcggtc gtgcggcggc tgttgccgtc
    23461 ctgatcctgc caggtgcggt gggtcagttt gccctccaca tacacgctca tgcctttttt
    23521 gaggctgctt tcggcgcgtt cggccaattg acgccaaact acaatctcat gccattctgt
    23581 ggtggtttgc cagttgccct ccctgtcttt gtaggattca ttggtagcta aggtgaactt
    23641 agcgaccacc gccccgtttt ccaagcgacg cacttcgggg tctttgccca gattgcctat
    23701 gagtatgact cgattgacca ttgttttaca gtttattaca agaggtttat acctcgcgct
    23761 gccgggtttt ccgcataacc ttcgacgcta ttttattggt tggcagcgcc cggtatgaca
    23821 aaaacggctt cgcgcttaca gcgcgagaca taaattgaac acattgtcct tcaaataccg
    23881 atcaataatt tttggaaacg caaacttaga caacttttcc cgttcaatcc ataaaaatcg
    23941 gctgtcttct ggaagaacaa tttcctgccg gggccgtatc tcaaaaaaaa tggccgnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaagg cggcgataca gacgctacaa
    24061 gtgcatctgt gttttgcaaa atctcgttcc agccggcatc cgactgcaaa acgtccaacg
    24121 cggtggtcaa cgcggggagt tccagcaacg gaaattcgta caatccattc cagatatctt
    24181 taccctctcg tctggaaatc agtactttgc ccgcctgttc tacgaccaga taatgaaaaa
    24241 agcgctcccg tttgacaatg cttttcgact tcaggggcaa gtccgacacc gtgccctctg
    24301 ccaatgcccg gcaatgctcc gacaccgggc actgcctgca aagcgggt
//
LOCUS       DAORLF010000016        24085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1155_length_24085_cov_2.030670, whole genome shotgun sequence.
ACCESSION   DAORLF010000016 DAORLF010000000
VERSION     DAORLF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24085
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1155_length_24085_cov_2.030670"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK198_02685"
     CDS             complement(<1..421)
                     /locus_tag="PK198_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37670.1"
                     /translation="MQLKRLNELLEQFKIHIRSAQHEESVMLWETQQLFQNNWDTEAP
                     RLAEMFRQSLENSISRRYWSRELYAPREMMLEFIALDEEMVRIMFRDLFNENKEVDGR
                     MDRFVFYCNEMLQAYKEKHQRPPHNRHYHDDNYTMVSL"
     gene            502..681
                     /locus_tag="PK198_02690"
     CDS             502..681
                     /locus_tag="PK198_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37671.1"
                     /translation="METKQEILEIVEKLPDEHLNELLEYPRRLEQSSFESTLVEKYLG
                     VIISEDKEVLENLAK"
     gene            678..845
                     /locus_tag="PK198_02695"
     CDS             678..845
                     /locus_tag="PK198_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37672.1"
                     /translation="MITLRQALDIHEALIEQFGGSRGVRDQNALTAALERPFSGFGEI
                     EFYPNPQEKAA"
     gene            1077..3464
                     /locus_tag="PK198_02700"
     CDS             1077..3464
                     /locus_tag="PK198_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF37673.1"
                     /translation="MKKTAQFLLFLALVCLSISVQAQTKRQWVKKGDDAYAINAFNTA
                     ITAYEEAMKKGANDEPDAVGRLADSYRQLNRLEEAERNYAVASKLKNVPPAYILQYAH
                     TLKGLGRYDEARRWYQEYARTNAVEGNHYAEGCDFAKAQRGVASAYSITNELINTGAS
                     EFSPALFGDRVVFASSRMDLQQPSGNWDGRVYNRLYVSSVGRSGGLDSPFFLKSDVRS
                     SNSEGPLAFSPDGRTVAFTRNNYVEGLRQIPSKAMQLSLFLADVGATGEWLNVRPFPY
                     NGGSDFSTGYPAFSPDGNTLYFASDRPGGFGGYDIYISFKTGTSWGAPENLGPVVNTP
                     GNELSPYFDGQSLFFSSDWHHGLGGLDVFRAEQANNRWARIFHLGQGVNSSYDDYGFI
                     FDGFKNMGYVVSNRPGGRGNEDIYKVARSADNITFRITNAADGSPIPNAVIDFTACGE
                     GVFQADARGLYTFQMVQGLNCEVLIRKEGFQNFIFPLSSITGQETREFDVRLLGTKDL
                     FYGSVIDQSTGRALAGVSVQSTNQFTGVTSEAFSDAAGSYALALNPNTVYVVRYSRAG
                     YQDVNRTVRTAAVVEPNTLGLMRLTSVTGGGTTGGGTTGGGTTTTPVGDNIPAGFSVQ
                     LASLKAPNVENFNNMGSIGTVYAKQEGGVYKIRVGVFATRQQAEAAQNTAKKQGYPQA
                     FIVEEKGGSATAVKGGTTTTTPSTGTGTTTGAATPSAYMIQLAAYRDARNFDDSKVRN
                     LGTVIDWPKGQFTAKVLTGFSTLDQARTALTRAKAAGFPDAYIVTQENGQLKKVN"
     gene            complement(3555..4148)
                     /locus_tag="PK198_02705"
     CDS             complement(3555..4148)
                     /locus_tag="PK198_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37674.1"
                     /translation="MKNKISIITFLMAFVVVGTVQAQIKVNPKVGFNASAVDAKVQNI
                     TAEARYGWNAGIDLRMGDGAVFFKPGLHYYNFTADLFQDVNQPGDVPMKDETTIQSLK
                     LPVNAGIRLTGDGGLLGVNVHGGVTPSYILGVREGATVPFVKDDLKPFTLGANLGLGI
                     DVLFFTVEANYEIGLTPFFNGAEGKNNVLSVSAGIKF"
     gene            4916..>5191
                     /locus_tag="PK198_02710"
     CDS             4916..>5191
                     /locus_tag="PK198_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRF37675.1"
                     /translation="MQNEATKILLEAIKQGRKPALQTVFDLHYAEVCRAIYKLVPDAA
                     LMEDLAQEVFIRFWEKREQIEVLESIGGYLHRMAVNEALAHLRRRRFF"
     assembly_gap    5192..5262
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5263..5466
                     /locus_tag="PK198_02715"
     CDS             <5263..5466
                     /locus_tag="PK198_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF37676.1"
                     /translation="HQDLSLRLQAALNTLPPRCRAVFQLSRFEEMSYREIADQMDISI
                     KTVENQMGKALKLLREQLSEFLQ"
     gene            5592..6065
                     /locus_tag="PK198_02720"
     CDS             5592..6065
                     /locus_tag="PK198_02720"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HRF37677.1"
                     /translation="MVESKIAALLEQRFQEPDLSDCFTVEVALKPGNRLEVAFDSDSG
                     VTFEKCKIVSRFLESHLDSEGWLGEKYMLDVSSPGISRPLVFLRQYPRNVGRTVEVTL
                     ADHSVHKGILIAADTETITVEEKVTLLENGKKKKTTVQTVIPFNNIHSTVVKVTF"
     gene            6078..7322
                     /gene="nusA"
                     /locus_tag="PK198_02725"
     CDS             6078..7322
                     /gene="nusA"
                     /locus_tag="PK198_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRF37678.1"
                     /translation="MVNLVETFSDFKAGKNIDRPTMVRVLEDVFRTLIRKKFGTDDNF
                     DVIVNTTKGDLELWRVRQIVPDGEVTDDRTQVSISEALAIDEDYEVGEECYEQLHLDD
                     FGRRSIMAARQTLISRIMELEKDEVFKRYTDRVGELIIGEVHQVLKKDIIILDDVSGN
                     ELLMPRTEMIKGDFFRKGDMVRGVIRKVEMKNNNPVIIVSRTDNLFLEKLLEQEVPEI
                     EDGLITVKNIVRLPGERAKVSVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDII
                     TYTNNTNLYIQRALTPAKVTSIDLDMDKKHADVFLDPDQVSLAIGKGGSNIKLASQLT
                     GFEIDVYRNNARYEEIDDVDMDEFRDEIEDWIIEAFKSIGCDSARSILSLSVDELLRR
                     TDLEEETIRDVMRILEAEFEEE"
     gene            7714..>7967
                     /locus_tag="PK198_02730"
     CDS             7714..>7967
                     /locus_tag="PK198_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37679.1"
                     /translation="MEKRLVKIAKELNVGTTTIVEFLHKNGYEIENKPTAWISDDMHT
                     ILVKEFQKSIDIKEQADQLVIGTRPAPKKDAPVVAKTDKP"
     assembly_gap    7968..8047
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8068..>9808
                     /locus_tag="PK198_02735"
     CDS             8068..>9808
                     /locus_tag="PK198_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRF37680.1"
                     /translation="MLQNRLKILGKIDLDAGKPKSKQEPATELPVAPVVVETPPPPPP
                     KPEVNEVADLPAIKEEAPVVVLPPVVEVAPPPPAPPVEEVKPVQLPVIEKKPVEPAPA
                     PVQEVAPPQEAGNVESEDAVIRAEAPELRGLKIMGKIDTSKFQPEKKKKKGKEPERKP
                     ASPATPASATPTTDDKTKKAAPAAANDDAARKRKRKRKKVATEPSGQDASRQQQQQPG
                     ARRKDGSAPPSGPEVKEVSQKEIEDKIKATIARLSGGGKKKRQKLQRDKRERMRERQE
                     MADMENETGKLQVTEFVTVSELASLMNVSPAEVIMTCLNVGVVVGINQRLDAEVIDLV
                     ATEFGFEIEFIGAEATLETEDEEEDDPADLVPLAPIVTVMGHVDHGKTSLLDHIRKSN
                     VVEGEAGGITQHIGAYEVDLGSGKKVTFLDTPGHEAFTAMRARGTKVTDIAVIVVAAD
                     DSIMPQTREAISHAQAAGVPIVFAINKIDKDGANPERIKQELASMDFLVEEWGGKYQS
                     QDISAKHGTGMDMLLEKILLEAEIQELKANPGRKATGTVIEASLDKGRGYVSKLLVQN
                     GTLNVGSAIVAGEH"
     assembly_gap    9809..9818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9819..10718
                     /locus_tag="PK198_02740"
     CDS             <9819..10718
                     /locus_tag="PK198_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRF37681.1"
                     /translation="IVAGEFSGKVKAMYNERGKNVKSAGPSTAVLVLGLSGAPTAGER
                     FKEVEHESEAREIAGRRAQIAREQTSRATKRISLDEIGRRLALGNFKELNLIVKGDVD
                     GSIEALSDSLIKLSIESVKVNVIHKAVGAITESDILLASASDAIIIGFQVRPSATARK
                     LAEREGVEIKLYSIIYEAIEEIKMAIEGMLEPTREEKIIGSVDVKEVFKITKVGTVAG
                     CQVAEGKILRNSPIRLIRDGIVTYPTKEGQTAEIASLKRVKDDVKEVRAGMECGIAIK
                     NFNDIKVGDVIEAYEIIETKQKL"
     gene            10772..>12464
                     /gene="thrS"
                     /locus_tag="PK198_02745"
     CDS             10772..>12464
                     /gene="thrS"
                     /locus_tag="PK198_02745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRF37682.1"
                     /translation="MQTINITFPDGNVRQYEKGVTAMQIAESISQGLARNVLSAKING
                     EVWDASRSIEDNATLQLLTWDDKEGKSTFWHSSAHLMAEALEALYPGIKFGIGPPVDN
                     GFYYDVDTGDRQLSTNDFAAIEAKMTELARAKSDYKRRDVPKDEAIRYFEEKGDEYKI
                     ELLQDLADGSITFYEQGNFTDLCKGPHIPNTEKIKAVKLLALAGAYWRGDETRKQMTR
                     IYAITFPKQKELEDYLHMLEEAKKRDHRKLGQELELFMFSERVGAGLPIWLPKGTQLR
                     ERLMNFLREEQEKRGYKMVITPHIGKKDLYVTSGHYEKYGSDSFQPIQTPREGETFLL
                     KPMNCPHHCEIYAFKPRSYKELPLRLAEFGTVYRYEQSGELHGLSRVRGFTQDDAHIF
                     CTPEQVKEEFLNVLDLTRTVLSKMGFEKFTAQISLRDPENPGKYIGSDENWNNAENAI
                     IEAAKEAELETVTELGEAAFYGPKLDFMIKDAIGRSWQLGTIQVDYNLPDRFKLEYIG
                     SDNQAHRPVMIHRAPFGSMERFISILIEHTAGKFPLWLAPEQYALLPISDRFIGYC"
     assembly_gap    12465..12474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12475..12727
                     /locus_tag="PK198_02750"
     CDS             <12475..12727
                     /locus_tag="PK198_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF37683.1"
                     /translation="KFHDYCREVEEKLALHDIRGFVDDRNETIGRKIRDTELKRIPIM
                     LIVGEKEVETGTVSVRRQGDGDKGAMSVEDFTSYFKGLL"
     gene            12865..13212
                     /locus_tag="PK198_02755"
     CDS             12865..13212
                     /locus_tag="PK198_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37684.1"
                     /translation="MKKTLLGILFCMFGLAAQAQFAYTGNAGDQRAGRSTEVTTTIQI
                     FPNPATEYFNVSSSQQVERISVYNLLGREMKSFTFTEGDRYFVGDLPKGMYLVQMTGK
                     DNRLISTQRLSKR"
     gene            complement(13596..>13905)
                     /locus_tag="PK198_02760"
     CDS             complement(13596..>13905)
                     /locus_tag="PK198_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37685.1"
                     /translation="VIRLNIGPLTSVQEPALAAGAVSVFPNPANEEVKVAFKLDAPSA
                     EVEVSIMDLSGKVLRTERHQNLFRDQLTINTAQLPAGAYNVRIRTEQGVAVQRVVVQH
                     "
     assembly_gap    13906..13983
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13984..>14265)
                     /locus_tag="PK198_02765"
     CDS             complement(<13984..>14265)
                     /locus_tag="PK198_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37686.1"
                     /translation="TFLYRWQGDANGDDQINLAEYGGAPLAFNSYEITGAETNIITIP
                     VDLDGNQIPLDDGYHYFIAVQYATEDDQFMFMAATQQLDYTGMWFYTDSL"
     assembly_gap    14266..14358
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14359..>14638)
                     /locus_tag="PK198_02770"
     CDS             complement(<14359..>14638)
                     /locus_tag="PK198_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37687.1"
                     /translation="SSVNYGSIAPDSTAENVFFTNEFTPPNAPGELYTGSYTIALNGA
                     TDEVASNDSRTFQFAVSDTVFSKEFNTGLVGLRYGGAGGTTRSYSWGNI"
     assembly_gap    14639..14650
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14651..>14904)
                     /locus_tag="PK198_02775"
     CDS             complement(<14651..>14904)
                     /locus_tag="PK198_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37688.1"
                     /translation="IQFEWIGGWDYFWAIDDIELLNQDLSIQNDMQVNTFFARAPNAI
                     TPASQLEPFGFVADVKNNGRLTAASSTLTVSIKNAGNTEI"
     assembly_gap    14905..14935
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14936..15499)
                     /locus_tag="PK198_02780"
     CDS             complement(<14936..15499)
                     /locus_tag="PK198_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37689.1"
                     /translation="MIKFFTRCFALVLLLAMGASHSYGQTPFYSEDFANGIPAGWTNV
                     DATSLAAQAVIFQHSTDPAAVAVAALGYTPSSTFGSATAANGYIWANSDRGLESAPAE
                     DHTTRLTTTAINCSGQNIVYLRLQSLIGVFDYDASTNVKIRVSNNNGTSWTDFVPFPC
                     LVTGAAAPPCTRWSANPEIVGINISSVA"
     gene            complement(15689..16666)
                     /locus_tag="PK198_02785"
     CDS             complement(15689..16666)
                     /locus_tag="PK198_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRF37690.1"
                     /translation="MIDWLAFDRLQALIVGDVMTDRYLIGSVERISPEAPVPVVQLRT
                     KDARLGGAANVALNVQAMGAKAWLCSAVGNDEAAEELLALIRQEGLPTQGIIGCPGRK
                     TAVKTRIMAGAQHLLRIDEETTEDISTEEAGLLLSGIAEILKNHPIDVVIFQDYNKGV
                     LTPEVIEAVTKLAMERNIPTAVDPKVKNFWAYRGVTLFKPNLKEIRNQYPSRIETTQE
                     SLNEAARYIQQKLDNQLVIITLSEKGLFCFGPNESGIIPAFPRIISDVSGAGDTVISV
                     AALCLALDMKLKDIALLSNIAGGQVCEKPGVVTVDKAALFEEYRRRSQE"
     gene            complement(16755..17186)
                     /locus_tag="PK198_02790"
     CDS             complement(16755..17186)
                     /locus_tag="PK198_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRF37691.1"
                     /translation="MIWKHHLSLTQVNSFAANTAMEHLGIEVTEIGPDFITAKMPVDH
                     RTKQPLGLLHGGASVLLAESLGSFASFLCIEDTSKYMSVGVEINANHLRSVSDGYVWG
                     TARPVRIGRLIQVWNIEIVDDQERLICTSRLTIAVVERLRN"
     assembly_gap    17389..17532
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17533..18120)
                     /gene="miaA"
                     /locus_tag="PK198_02795"
     CDS             complement(<17533..18120)
                     /gene="miaA"
                     /locus_tag="PK198_02795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRF37692.1"
                     /translation="MHSKHLIVIGGATATGKTAFAIRAALHFQTEILSSDSRQFYKEM
                     NIGTAKPTEEELAAAPHHFISHLSIHQPYSVGDFEREALACLHQIFETRDVAVMTGGS
                     GLYIKAVCEGLNSFPEVPESIRAEVQNMYDLKGIAALQDELKQADPDYFAEVDTNNPA
                     RLLRAVAVCRAAGRPFSSFRNQPLEPRSFQPVYVQL"
     assembly_gap    18300..18338
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18356..>18813
                     /locus_tag="PK198_02800"
     CDS             18356..>18813
                     /locus_tag="PK198_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF37693.1"
                     /translation="MEPCILACTLGRPLYFLVRGDVFAKPFYAAILKAFHMLPMYRLK
                     DRGYKFVKENFQTLDACFDALHENKTIMILAEGTTIAEKRLRPIKKGTARLAFGALDK
                     YPDMEEVHVVPVGVNFDYMDRFRAEVMIDFGHPIKARDYYEMYRANGSEGT"
     assembly_gap    18814..18829
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18830..19487
                     /locus_tag="PK198_02805"
     CDS             <18830..19487
                     /locus_tag="PK198_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37694.1"
                     /translation="ELAGRMRSLIVNVEKQSDDALAEQLLVMSRSLRRFTFLPVLGKD
                     PALLQQEQLVANYINTLAPTEKSALTGACGQYFEALQKGGVSDRAVAEGKNDSLSEKL
                     LLLAGLPVFLAGFVFNFLPARLARYIADKQIESVEFYAPVMLVLAMAAYLLYYAGWGL
                     FLGWKGLWLLPLAALAGYFSVLYREAFQLWKYRRRWAALPEDRQRSLHALRADCSLNV
                     "
     gene            complement(19497..>20100)
                     /locus_tag="PK198_02810"
     CDS             complement(19497..>20100)
                     /locus_tag="PK198_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRF37695.1"
                     /translation="NGQYQMVVHQPRMRLRQLVNHPRMILDDYRQGSGKFQDVLEEWD
                     VIRRSGHKVLMFSSFVSYLDLFKQALQEAGLPLAWLSGDLSSTQRQAEIARFQNDPQV
                     QAFLISIKAGGTGLNLTAADYVFVLDPWWNPFVEQQAIARAHRIGQDKNVIALKFITR
                     GSIEEKILVLQDRKKQLAEDIIAHTEKLSLQRDELAFLLE"
     assembly_gap    20101..20215
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20216..22422)
                     /locus_tag="PK198_02815"
     CDS             complement(<20216..22422)
                     /locus_tag="PK198_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRF37696.1"
                     /translation="MFPNTRTTIVYNLCELLPGLYGMNAFLVQQERDGALTHIVQKAA
                     LHILDSFKVEEVEQHRRLLRIVEQIQAKALEQYFNGKAKKPRPLAELYEDEKLKKQID
                     QYVQRRIDELLSLVQKYGFAVSWDAERRSLVKDMVLQFADAPLQPRLSFSRLPEGVSY
                     RLKLAEAGGKVWNIQSREVASVCNQPAWIFADYRLYRVEHINGNMVLPFRNKNEVRIP
                     KSSVKTYFQRFILKVAAKADIEAEGFEIIRHNQLKTCRLEAVHDLFSGQWALTAYMAY
                     PGAEFACRDTRQERTSLEFGNGDDIRILKVQRHRDEEECYMEALRQLGLIAGNGSQFV
                     LPHPAEDSWQLLQWLCEHRRELEAEGFTVTTPETEQGKLLTETAHLEWNTQQGNDWFD
                     LNGEVRIGPHTIPFLLLAPYIREGNRLYPLPNGEVFLIPAEWMSRYKGLAQMGRKQGE
                     QLRIAKNQIGLLQDAGLMDEEALSNAPTNNETPLPAGLRAELRPYQLQGFQWLARLHG
                     EGLGACLADDMGLGKTLQTIAVLLYAKTQKPAAEAPSAGQMSLFQPAADVDFLRPLQA
                     LVVLPASLVFNWQRELAQFAPSLSVYVHTGAGRHKDIRLLARFDVVLTTYQTALRDSS
                     LLLKTEWEYIVLDESQQIKNKDSQVFKTLNSLSARHKVSLSGTPIENSLSDLWSQMQF
                     INPELLGTHAFFKKAFILPIEKQQDEAQKNRLRTLVQPYLLRRTKEEVAKDLPP"
     gene            complement(22687..>23387)
                     /locus_tag="PK198_02820"
     CDS             complement(22687..>23387)
                     /locus_tag="PK198_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37697.1"
                     /translation="GSLTAQVTSISGSALGWAGIVMRESNAPGAKKAQLMTNLSSLHR
                     REFRTTTNGAAIPQQSASNNRYWLRITRAGNQFTMSVSANGTSWFLIGAQTIAMNNCI
                     EMGLVATNYTANSTVTATFAGVSYTGSNATATSFDHALHGASLDTPYSFEVYPNPTGG
                     ELNLDLTQYAGRSVRIETYSLEGKLLQFAELDEVQNTVERFDLKGFQSGMYLLKVKSA
                     GLPDATRRVVVNRN"
     assembly_gap    23388..23414
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23415..>24085)
                     /locus_tag="PK198_02825"
     CDS             complement(<23415..>24085)
                     /locus_tag="PK198_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF37698.1"
                     /translation="INGLTNTCTFTVTKVDNTAPTITCPATQTLALGANCTAVLPDYT
                     NLATTGDGCGVQNVTQSPEAGTTISGAGNMSVTLTVTDINGLTNSCTFTVTKVDNTPP
                     MVQCFNQTLNFNGEESIALDTDDLAEVSDNCGVQSISLSPNTISAERVGQIVPVTVTA
                     TDINGNTATCTSNITVSGFPAGWSQQPGGAGCSGNNNAYNSGTGVWTATSTNCFYGPP
                     YTSDAT"
BASE COUNT         5854 a         5679 c         6151 g         5675 t
ORIGIN      
        1 acaacgacac catcgtgtaa ttgtcgtcgt gataatgccg gttgtgcgga ggccgttggt
       61 gtttttcttt atatgcctgg agcatttcgt tgcaataaaa tacgaagcga tccattcttc
      121 cgtccacttc tttgttttcg ttgaacaagt cgcggaacat gatgcgcacc atctcttcat
      181 caagagctat gaattcgagc atcatttctc tgggggcgta caactcgcgg ctccagtacc
      241 gccgggaaat gctgttttcc aatgattgcc gaaacatttc ggcgagcctg ggggcctcgg
      301 tatcccagtt gttttggaac aattgctgcg tttcccacag catgacagat tcttcatgtt
      361 gtgcgcttcg gatgtgaatt ttgaactgtt cgagcagttc gttcaggcgt ttgagttgca
      421 tggaaaaaac tgtagtggta aaggaatcgg aaaagaacca aagacgagag ctaaatgttg
      481 tgtttgggaa attcaaatga catggaaaca aagcaggaaa tattggaaat tgttgaaaaa
      541 ttgcccgatg agcatctcaa tgaattattg gaataccctc gtcgtttaga gcagtcaagt
      601 tttgaaagca cgcttgttga aaagtatctt ggcgtcatta taagcgaaga taaagaagta
      661 cttgaaaatc tggcgaaatg attacgcttc gacaagcgct ggatatccat gaagcgctga
      721 tcgagcaatt cggaggttct cgtggagtta gagatcaaaa cgcattgacg gccgcattgg
      781 aaagaccctt cagcggcttc ggcgaaatcg aattttaccc caatccccag gaaaaagcag
      841 catgattgaa aatttaggta ccgagagcgc gtaagtgtaa gactggaagg gacaatgttt
      901 caaaaaacag catcaaacaa aacttttccg cccaaagatt atcaataatt tgtttttttt
      961 tgaaatttgc cctaaattag ccaaaacaac tcaaggcatg aaggtgagtc gggcattatt
     1021 ttacgtcata caatagcccg gcgcagcaac ataccaacac caaacaatta caatcaatga
     1081 aaaagacagc tcaatttctg ctcttcctgg cccttgtttg cctctccatt tcggtgcagg
     1141 ctcagaccaa acgtcaatgg gtaaaaaaag gagacgatgc ctatgccatc aatgctttca
     1201 acacggccat tacagcctat gaagaagcca tgaaaaaagg cgccaacgac gaacccgatg
     1261 cggtggggcg cttggccgac tcttatcggc aactcaatcg cctcgaagaa gccgagcgca
     1321 attatgccgt agcatcgaaa cttaaaaacg tcccgccggc atacattctg caatacgcac
     1381 acaccctcaa aggtctgggc cgctacgatg aggcccgccg ctggtatcag gaatacgccc
     1441 gcaccaatgc cgtggaggga aatcactatg ccgaaggctg cgattttgcc aaagcgcaac
     1501 ggggagtggc atcagcttac agcattacca atgaattgat caataccggc gcttccgaat
     1561 tctcgccggc cctcttcgga gaccgggtgg tatttgcctc ttcccgtatg gatttgcagc
     1621 agcccagcgg caactgggac ggcagggttt acaaccgctt gtatgtttca tctgtaggcc
     1681 gttcaggtgg cctcgactct ccattctttc tcaaaagcga tgtgcgcagt tccaacagtg
     1741 aaggcccctt ggcattttca cccgacggcc gcacggtggc ttttacccgc aacaactatg
     1801 tggaaggctt gcgccagatt ccctctaaag ccatgcaact gagcctcttt ttggccgacg
     1861 tgggcgctac cggcgagtgg ctgaacgtgc gcccgtttcc gtacaatggc ggcagcgatt
     1921 tctctaccgg ctatcctgct ttttcgcctg atggcaatac gctgtacttt gcatccgacc
     1981 gccccggagg cttcggagga tacgatatct acatttcttt taaaacagga acaagctggg
     2041 gtgctcccga aaacctcggc ccggttgtaa atacgcccgg caatgaactg tcgccgtatt
     2101 ttgacggcca aagtctgttt ttctcttccg attggcatca cggcctgggt ggcctggatg
     2161 tattccgggc agagcaggcc aacaatcgct gggcgcgcat tttccatctt ggacagggcg
     2221 taaactcttc ttacgatgac tacggattca tttttgacgg cttcaaaaac atgggctatg
     2281 tggtttccaa ccgccccggc ggccgcggca atgaagatat ctataaagtg gcccgcagcg
     2341 ccgacaacat taccttccgc atcacaaatg cggctgacgg cagcccgatt cccaatgccg
     2401 tcattgattt taccgcttgt ggcgaaggtg tttttcaggc cgatgcgcgg ggtttataca
     2461 ccttccaaat ggtgcagggg ctgaattgcg aagtattgat tcgcaaagaa ggattccaga
     2521 atttcatttt ccccttgtcg tccatcaccg gacaagaaac ccgcgaattt gatgttcgtc
     2581 tgctgggtac caaagatttg ttttacggct ccgtcattga ccaaagtaca ggccgggcat
     2641 tggcgggcgt gagcgtacaa agcaccaatc aatttacagg agtgacctct gaggcgtttt
     2701 cggatgcggc aggttcttac gcgctggcgc tcaatcccaa tacggtatat gtggttcgat
     2761 attcccgtgc cggctatcag gatgtgaacc gcacggtgcg tactgctgcc gtagttgaac
     2821 ccaatacgct gggccttatg cgtttaactt ctgtaactgg tggcggcaca accggaggcg
     2881 gcacaacagg tggtggaact actaccaccc cggttggtga caatattccg gcaggttttt
     2941 ctgtgcagtt ggcttctcta aaggcgccca acgttgaaaa tttcaacaat atgggtagta
     3001 tcggaacggt atatgccaaa caggaaggcg gagtttacaa aatcagagta ggcgtttttg
     3061 ctacccgcca acaggctgaa gctgcgcaaa atacggccaa aaaacagggc tatccgcaag
     3121 cttttattgt ggaagaaaaa ggcggcagcg ccacagcggt gaaaggcggt actaccacaa
     3181 ccactccttc tacgggtacg ggcactacta ccggcgctgc tactccgagc gcctacatga
     3241 ttcaattggc cgcttaccgc gatgcccgta attttgacga ttccaaagtc agaaacctcg
     3301 gcacggtcat tgactggccc aaaggtcaat ttacagcaaa agtactcacc ggattcagca
     3361 cgctcgatca ggccagaaca gcgcttacaa gagctaaagc tgccggtttt cctgatgctt
     3421 acatcgttac gcaggaaaat ggacagttga aaaaagtgaa ttgacatctg aaaaagccat
     3481 aaaaaaagag aaaaggccgt agtagagtcc atccactacg gccttccttt tcccttttta
     3541 cgggaataat tcacttaaaa cttaatcccc gcgctcacgc taagcacatt gttcttgcct
     3601 tctgcgccat taaaaaacgg agtaagtccg atttcatagt tggcttccac cgtgaaaaac
     3661 aacacatcta tacctaagcc cagattggcc cctagcgtaa atggtttgag atcatctttt
     3721 acaaaaggaa ccgtggcgcc ttctctgacg cccagaatat aggacggggt aactcctcca
     3781 tgcacattta ctcccaataa gccgccatcg cctgtgagcc ggatgcctgc attgacagga
     3841 agtttcaggc tttggatggt cgtttcgtct ttcatgggca cgtcgccggg ctgattgaca
     3901 tcctgaaaga ggtcggctgt gaagttgtaa tagtgcaaac ctggtttgaa aaagactgcg
     3961 ccatccccca tgcggaggtc aattccggcg ttccagccat agcgggcctc ggctgtaatg
     4021 ttttgtactt ttgcatctac cgcagaagca ttgaagccta ctttcgggtt tactttgatt
     4081 tgagcctgta cggtacctac aaccacaaat gccatcaaaa aagtgataat gctgatcttg
     4141 ttcttcatga ctttctttgt tttggtttga aatcggtgct aaaacgaatc tatcatgcag
     4201 aataatgtta gaagaatcaa aaagtgccgt taagacgggg ttaatgcagt cggcttattg
     4261 acaaaagtca ccatcgggga tgataatagt catgtttcag cgactttgaa ggcgggtatc
     4321 tttgagccga atcttagaag atcagtcaat agacatctaa gatatcatgg tcaattgggt
     4381 gttcaatcgt tttggagccg gggtctttat cccggctttt tttgttcgta tggtattaat
     4441 tttttgttaa tggcattttt atgttgtgtg tattccgggg aagcattaca tttgccttcg
     4501 agagtttttt tataatccta ttttttaaca caaaattttc tttcccatga acacacattc
     4561 aaacttccgg cgccaaggtg gcgaaacgag tatcctaagc aatgccgaaa cagccgatcc
     4621 accatagcgc tgcgccgcaa tccgaccgag gaagagacgt tggagtcatc ctcatcaata
     4681 ctcggaccat atatataagg tgaagagtgt ttaataatga gaaataacgg gcaatcccga
     4741 cccggccaac cagagcgttt tgtcagttga tgatgaagcg ctctgttgtt ttttagagcg
     4801 gcttttcatc acctgatgca atggaccgct cgcttactca tcaaaaaaaa tgagaccttt
     4861 gtcgaaccat taataaagtt tacgccctgc gtaccggaat ttgcgccctc aagtcatgca
     4921 aaacgaagca acaaaaatct tactcgaagc cataaagcaa ggacgaaagc cggcgctgca
     4981 aacggtattt gatttgcatt acgccgaggt ttgccgcgct atttacaagt tggttcccga
     5041 tgctgccctg atggaagacc ttgcacaaga ggtttttata cggttttggg aaaaaagaga
     5101 gcaaatagaa gtgctggaat ccataggagg atatctgcat cgcatggcgg tcaatgaagc
     5161 gttggcacat cttcgccggc gccgtttttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatcaaga tttatcgctc
     5281 agattgcaag ctgcgctgaa cactttgccg ccccggtgcc gggctgtgtt tcagttgagc
     5341 cgctttgaag aaatgagcta tcgggagatt gccgaccaaa tggatatttc cattaaaaca
     5401 gttgaaaatc agatgggaaa agcgctcaag ttgttgcggg aacagctcag cgaatttttg
     5461 cagtgaagaa tttttcagga aatattgcaa attcctgaat ttcagtatat ctttgcccca
     5521 tcatttggga ataaaacaaa agagggagtg ccgaaacact ccctcttttc gttacaaata
     5581 cttgataatc agtggtagaa tcgaaaatag cagctttgtt ggagcagcgg tttcaagagc
     5641 cggacctttc ggattgtttc accgtagaag tagcgctcaa gcccggcaac cgccttgagg
     5701 tagcgtttga cagcgattcg ggcgttacct ttgaaaagtg caaaatcgtc agccgttttc
     5761 tggagtctca tttagattcc gaaggctggc tgggcgaaaa atatatgctg gatgtttcct
     5821 cgccgggtat cagtcgcccc ttggtcttcc tccggcagta tccgcggaat gtgggcagaa
     5881 cagtggaagt aacactcgcc gatcactctg tacacaaagg tattctgatc gcagcggata
     5941 ctgaaaccat taccgtagaa gaaaaggtga ccttacttga aaacggtaag aagaaaaaaa
     6001 caacagtgca aactgtcatt ccttttaata acattcattc aacagtagta aaagtgacct
     6061 tctaaacaga atttgtcatg gtaaatttag tagaaacttt ctccgacttc aaagccggaa
     6121 aaaacattga ccgccctacg atggtacggg tgctcgaaga tgtatttcgt accctcattc
     6181 gcaaaaaatt cggtaccgac gataatttcg acgtcatcgt taatacgacc aagggtgacc
     6241 ttgagttgtg gcgcgttcgg cagatcgtac ccgacggcga ggtgacagac gaccggactc
     6301 aggtgtctat atctgaagct cttgccattg atgaagacta cgaagttggg gaggaatgtt
     6361 acgagcagtt gcacctggat gattttggcc gccgttccat tatggctgcg cgccaaaccc
     6421 tcatctcccg cattatggag cttgaaaaag atgaggtctt caagagatac acagatcgcg
     6481 taggcgagct tatcattggt gaagtccatc aggtactcaa aaaggacatc attattttgg
     6541 atgatgtgag cggcaatgaa ctgctgatgc cacgcaccga aatgatcaaa ggcgatttct
     6601 tccgcaaagg cgacatggtg cgcggcgtca ttcgcaaggt cgaaatgaag aacaacaacc
     6661 cggtgatcat cgtctcccga acagacaacc ttttccttga aaaactgctc gaacaggaag
     6721 tgcctgaaat cgaagacggc cttatcaccg ttaaaaacat cgtccgcttg ccgggcgagc
     6781 gcgccaaagt ttcggtagaa tcttatgatg atcgcattga tcctgtggga gcttgcgtgg
     6841 gcatgaaagg ctcccgcata cacggcatcg ttcgcgaatt gcgcaacgaa aacattgaca
     6901 tcatcaccta caccaacaac accaatctgt acattcagcg ggcactcaca ccggctaaag
     6961 tgaccagcat agatttggac atggataaaa aacatgcaga cgttttcctc gatccggatc
     7021 aggtatcatt ggctatcgga aaaggaggtt ccaatatcaa attggctagc caactaactg
     7081 gttttgaaat tgatgtatat cgaaacaatg cccgttacga ggaaatagac gatgtggata
     7141 tggatgaatt cagagacgaa atcgaggact ggatcatcga agctttcaag agtattggtt
     7201 gcgactctgc gcgtagcatc ttgtctctca gcgttgatga gttgttgcgc cgcaccgacc
     7261 ttgaagagga aaccatccgc gatgtcatgc gaattctgga agcagaattt gaagaagaat
     7321 aatatcagga gtgataaaaa atgaaggttc atcttcgtca aagagtcaga taagtaaacg
     7381 attcatttta agtgccttat caattctttg gatggaagaa aatgaacctt cgtttttgct
     7441 ctgctcccaa gaagcgaagg tcgcaaaata tacattgcca aatttagtgt ggtttgttaa
     7501 gaatcatcgg tttttcattg tatctttgcc gccgttgaat cgcacgggct gagttttcgg
     7561 gtgttttcag ttaatcattg cacgcaggta aaaacttttt acaatggacc tttccttatt
     7621 atcactaaaa tgataagcgt tatgtgaagg aattcatagc cggtaaaagt ctttgcctgt
     7681 ctgctaacgt tgccaaagat tgcaaaacta tatatggaga aacgtttagt taagatagct
     7741 aaagagctta atgtaggaac gaccaccatc gtggaattcc tgcataagaa cggctatgaa
     7801 attgaaaaca agcctacggc ctggatcagc gatgacatgc acacgatttt ggtcaaggag
     7861 ttccaaaagt ctattgacat taaggaacaa gcggatcaat tagtcatagg cactcgcccg
     7921 gcgcctaaaa aagatgcccc tgttgtcgca aaaaccgaca aacctcannn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnagg aggaaaaacc gcgcggattg ctccaaaatc gtttaaaaat cttaggcaag
     8101 atagacttgg atgccggcaa acccaagtcc aagcaagaac ctgctaccga gctgcctgtt
     8161 gcgccggtag tagtggaaac accgccgccg ccacctccca agccagaagt taatgaagtg
     8221 gcagacctgc ccgctatcaa agaggaagcg cctgtggtgg ttctgcctcc tgtagtcgag
     8281 gtcgcgcctc cccctcccgc ccctcctgtc gaagaagtga agcctgtgca attgcctgtg
     8341 attgaaaaga agccggtaga gcctgctccc gcacccgttc aggaagttgc gccgccacag
     8401 gaggcaggca acgttgaatc tgaagatgcg gtcattcgcg ccgaagcgcc cgaattgcgc
     8461 gggttgaaaa ttatgggtaa aattgatacc tcgaagtttc agcctgaaaa gaaaaagaaa
     8521 aagggcaagg agcctgaacg caaacctgcg tctccggcta cgcctgcttc agcgactccg
     8581 actacagacg ataaaaccaa aaaagctgct cctgccgctg ccaacgatga cgcggccaga
     8641 aaacgcaagc ggaaacgcaa aaaggtggct acggagcctt ccggccaaga tgcgtcgcgt
     8701 caacagcagc agcagcccgg cgctcgtcgg aaagacggaa gcgctccgcc ttcaggtccg
     8761 gaggtgaagg aggtctcgca gaaagaaatt gaagataaaa tcaaggctac cattgcacgt
     8821 ctttccggcg gcggcaagaa gaaacgccaa aaactccagc gcgacaagcg ggaacgcatg
     8881 cgcgaacgcc aggaaatggc tgatatggag aatgaaaccg gaaaattgca ggtcactgag
     8941 ttcgtcacag tgtccgaatt ggccagcctg atgaacgttt ctccggcaga ggtcatcatg
     9001 acctgcctca atgtcggcgt cgtagtggga atcaaccaac gcttagatgc ggaagttata
     9061 gacctcgttg ctaccgaatt cggttttgaa attgagttta taggcgccga agcaaccttg
     9121 gaaacagaag atgaagagga ggatgatccg gcagatttgg taccgcttgc gcccattgta
     9181 acagtgatgg gccacgttga ccacggcaag acctctttgc tcgaccacat acgtaaatcc
     9241 aacgtggtgg aaggcgaggc cggcggtatt acccagcaca tcggtgctta tgaggtggat
     9301 ttgggcagcg gcaaaaaagt cactttcctc gatacgcctg gccacgaagc cttcactgct
     9361 atgcgtgctc gtggtactaa agtaaccgat atcgccgtga ttgtggtagc cgccgacgac
     9421 agcatcatgc ctcaaacgcg ggaagccatc agccacgcac aagcagccgg cgtgcccatc
     9481 gtttttgcca tcaacaaaat tgataaagac ggtgcaaacc ccgagcgcat caagcaagag
     9541 cttgcttcta tggacttctt agtagaagaa tggggtggga aataccaatc gcaggacatc
     9601 tcggccaaac atggtaccgg tatggatatg ctgttggaaa aaatcttgct tgaagctgag
     9661 attcaggaac ttaaggccaa tccaggcaga aaggcaaccg gtacggttat tgaagcctct
     9721 ttggacaaag gccgggggta tgtaagcaaa ttattggtac aaaacggcac cctcaatgta
     9781 ggctctgcca tcgtagcagg cgaacacgnn nnnnnnnnat cgttgcgggc gagttctccg
     9841 gcaaggtgaa agccatgtat aacgaacgtg gcaaaaacgt caaatcagca ggaccttcta
     9901 cggcagttct tgtgttggga ttgagtggcg ctcctactgc cggcgaacga tttaaagagg
     9961 ttgaacacga atccgaagcc cgcgaaattg ccggccgtcg ggcgcaaata gccagagaac
    10021 agaccagccg cgctaccaag cgcatcagct tggacgagat cggacgccgt ctggctttgg
    10081 gcaacttcaa agagttgaac ctcattgtga aaggcgacgt ggatggctcc attgaagcgc
    10141 tttcagattc tttgatcaaa ctttctatcg agtcggtcaa agtcaatgtg atacacaaag
    10201 ccgtgggcgc catcacagag tccgatattt tgctggcctc tgcttctgat gccatcatca
    10261 tcggcttcca ggtacgcccc agcgcaactg ccagaaagct ggctgaacgc gaaggtgtgg
    10321 aaatcaagct gtactccatt atttacgagg ccattgagga aatcaaaatg gccattgagg
    10381 gcatgctcga acccacacgc gaggaaaaaa tcatcggcag cgtggacgtt aaggaggtgt
    10441 tcaaaatcac aaaagtgggt actgttgccg gttgccaggt ggcggagggc aaaattttgc
    10501 gcaactctcc cattcgcctt atccgggacg gcatcgtgac ttatcctacc aaagaaggcc
    10561 agacggcaga aatcgcctcg ctcaaacgcg tcaaagacga tgtaaaggaa gtgcgcgccg
    10621 gtatggaatg cggtattgcc atcaagaatt tcaatgacat taaagtcggc gacgtcattg
    10681 aggcttacga aatcattgaa acgaaacaga aattgtaaat caatcagcct aagctgcaac
    10741 attcagcagc gaggcctttc ttagtcaaca catgcaaacg atcaacatta cattcccgga
    10801 cgggaatgtc cggcagtacg aaaaaggcgt aactgccatg caaattgcgg aatccatcag
    10861 tcagggactt gcccgcaatg tgctgtctgc caagataaac ggcgaggttt gggatgcgtc
    10921 gcgttccatc gaagacaatg ctacactgca attattgacc tgggatgaca aagagggaaa
    10981 atctactttc tggcattctt ctgcgcactt gatggcagaa gccttggagg cgttgtatcc
    11041 aggcatcaag tttggtattg gtccgcctgt tgacaatgga ttttattatg atgttgatac
    11101 gggcgaccgt cagctttcca ccaatgattt tgctgctatt gaagccaaaa tgacggagtt
    11161 ggcgcgtgcc aagagtgatt acaaacgccg ggacgtgccc aaggatgagg ccattcgcta
    11221 ctttgaagaa aagggcgatg agtacaaaat cgagttgttg caagacttgg ccgacggctc
    11281 catcactttt tacgaacaag gtaattttac cgatctttgc aaaggtccgc atattcccaa
    11341 cacagagaaa atcaaagcag taaagctgtt ggcgttggcc ggcgcttatt ggcgtggcga
    11401 cgagaccaga aaacagatga cgcgcattta tgccattact ttccctaagc agaaggagtt
    11461 ggaagactat ctgcacatgt tggaggaggc taaaaaacgc gatcaccgca agctggggca
    11521 agagttggaa ctcttcatgt tctcagaacg cgtaggcgca ggcctgccga tttggttgcc
    11581 caaaggcaca caactgcgcg agcgactgat gaatttcctt agggaagagc aggaaaaacg
    11641 gggttacaaa atggtcatta cgccacatat cggcaaaaaa gacctctatg ttacttccgg
    11701 ccattatgaa aaatatggct cagacagctt ccaacccatt cagacgccga gagaagggga
    11761 gacctttctg ttgaaaccga tgaactgccc gcaccactgc gaaatttacg ccttcaaacc
    11821 ccgctcttac aaggaattgc cactgcgatt ggcggagttc ggcactgtat atcgctacga
    11881 gcagagcggc gagttgcacg gcttgtccag agtgcgcggt ttcacacaag acgacgcaca
    11941 cattttttgc acgccggaac aggtgaagga ggagttctta aatgtgctcg accttactcg
    12001 aaccgtactg tcgaaaatgg gatttgaaaa attcacagct cagatttctt tgcgcgatcc
    12061 cgaaaatcca ggcaaataca tcggctccga tgaaaattgg aacaacgccg aaaacgcaat
    12121 tattgaggct gctaaagaag ccgaattgga aacagtaacc gagttgggag aagctgcgtt
    12181 ttacggcccc aagttggact tcatgattaa agatgccatt ggccgttcct ggcaattggg
    12241 tactattcag gtggactaca accttccgga tcggttcaaa ttggaatata tcggttccga
    12301 caatcaggcg catcggccag tgatgattca ccgggcgcct tttggctcca tggagcgttt
    12361 catcagcatt ttgattgaac acacagcagg caaatttccg ctttggctag cgccggaaca
    12421 gtatgctttg ttgccgataa gtgatagatt tatagggtat tgcgnnnnnn nnnncaaatt
    12481 tcacgactac tgccgcgaag tggaagaaaa attggcctta cacgacatca gaggttttgt
    12541 agatgaccga aacgaaacca tcggcaggaa aatacgcgat accgaattga agcgcattcc
    12601 catcatgctg atagtgggag aaaaagaagt agagaccggc actgtatcgg tgcgccgcca
    12661 gggagacggc gataaggggg caatgtctgt ggaagacttt acaagttatt ttaaggggct
    12721 tttgtaagca aagtctttga gagaaagtga ctttattgtc ggccaaacgt taaaattttg
    12781 gtaaacgttt tgtctggtag ttgtgttgta tgaaaaaaac aactactttg cgccatcttt
    12841 aattatttga acaatccgat ccgcatgaag aaaactttac ttggaatcct attttgcatg
    12901 tttggtctgg ctgcccaggc tcaatttgct tataccggca atgctggaga ccagcgcgct
    12961 ggtcgctcga cagaagtgac gacaaccatt caaatctttc ccaatcctgc tacggagtat
    13021 ttcaatgtaa gttcctctca gcaggtagaa agaatttctg tgtataattt gttgggtcgc
    13081 gaaatgaagt cgtttacctt cacggaagga gatcgttatt tcgtcggtga cctccccaaa
    13141 gggatgtatc tcgtacagat gacaggaaaa gataaccggc tgatttctac gcagcggttg
    13201 tctaagcgtt aattatatat atcgtgttcg cggattgggg taagaagtaa aacatgccct
    13261 gatttcataa agcgcccttt tctcttatgc gggaaaaggg cgttttgcat ttccagaatt
    13321 aggccaatca gtttttggtt aaccatcgtc atcgtgctta gcacacacct ttaacggtat
    13381 ctgggccgcc tggcatttca cataaaagtg tagcgtggcc ctgaaaatgt gtactttatt
    13441 ttggccctgt tccttacgag ctaacgtaag catgggggac aacctttttt caactctata
    13501 aaacaggtgc ccctcaaaaa aatcccggac ttcagcgtac tgaaatccgg gatgtcgctt
    13561 ccgatcagat cgaaaacgta aggtatgtca acagtttagt gttgtactac aacacgctga
    13621 acggcaacgc cttgctcggt acgaatgcgt acattatagg cgcccgcagg cagttgagcc
    13681 gtgttgatgg taagctgatc gcggaacaag ttctgatggc gttctgtacg aagcactttt
    13741 ccagacaagt ccatgatgct tacttccacc tcagcagaag gagcgtcaag tttgaaagca
    13801 actttcactt cttcgttggc cgggttgggg aacacgctca ctgcgccggc tgccagcgcc
    13861 ggctcctgca cggaggtgag cgggccgata ttgaggcgga taaccnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnagagagt cggtgtaaaa ccacataccc gtataatcaa gctgttgggt agctgccata
    14041 aacataaatt ggtcatcttc agtggcgtac tgtactgcta taaagtagtg gtagccatcg
    14101 tccaacggaa tctgattgcc gtcaagatcc acagggatgg tgatgatgtt ggtttctgct
    14161 ccggtaattt catacgagtt gaaagcgagc ggagcgcctc catattcggc aagatttatc
    14221 tgatcgtcgc cattggcatc gccttgccag cgatacagga aggttnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnaa atattgcccc aagagtagct tcttgttgta ccgcctgctc
    14401 cgccataacg aagacctacc agaccagtat tgaattcttt ggagaaaaca gtgtctgaaa
    14461 cagcaaactg gaaggtgcgg gagtcgttgg acgctacctc atcagtggcg ccattcaatg
    14521 cgatggtgta ggagccggta taaagctcgc cgggtgcatt cggcggcgtg aattcattcg
    14581 taaagaatac gttttcagcg gttgaatcag gagcgataga accgtaattg acagaagann
    14641 nnnnnnnnnn atatctctgt gttgcctgca tttttgatag aaactgtcag tgtagagctg
    14701 gctgcggtca gcctgccatt gttcttgaca tcggcgacga aaccaaacgg ctccaactga
    14761 gaagcgggcg tgatggcatt cggggcgcga gcgaagaatg tattgacctg catatcgttc
    14821 tgaatgctga ggtcttgatt gagcaattcg atatcgtcaa tagcccagaa ataatcccaa
    14881 ccgccaatcc attcaaattg aatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcta
    14941 cagaactgat attgatgcct acgatttcgg ggttggcaga ccaacgcgtg catggaggag
    15001 cggcagcgcc tgttaccagg catggaaaag gaacgaagtc ggtccagctt gtgccgttgt
    15061 tgttgctcac tctgattttt acattggtgg aagcatcgta gtcaaatacg ccgatcaaag
    15121 attgtaagcg cagatataca atgttttggc cggaacagtt gatagcggtc gttgtgagac
    15181 gagtcgtatg atcttccgca ggcgcgctct caaggccgcg gtctgaattg gcccagatgt
    15241 aaccattggc agcagtagct gagccgaaag tcgagctggg cgtataaccc aaagcggcta
    15301 ctgctacggc agcgggatcg gtggagtgct gaaagataac agcctgagcg gcaaggctgg
    15361 tggcatcaac gttggtccag ccggcaggga tgccgttggc aaaatcttcc gagtaaaaag
    15421 gtgtttgccc atagctatga gaagccccca tagcaagcag caacaccaat gcgaaacagc
    15481 gggtaaaaaa tttgatcatc ggtttgaaaa tttaatgtga gaaagtacat aattcaaatc
    15541 ccaaaagtac agcctttcgg caaaactgca aggaaaaaga aaaaaagtgc agatgggcga
    15601 cattatttgc atggggcata gagcatagcg ggcagttgga acgaagcgac atcccgatgc
    15661 ggaacgaaac cgagaggata tagaggaatt attcttggct gcgccgccga tattcttcaa
    15721 agagggccgc tttgtcaaca gtgacgacgc cgggtttttc gcaaacctgc ccgccggcga
    15781 tattggacag cagggctata tccttgagtt tcatatctaa agccagacac agcgctgcaa
    15841 cgctgatgac cgtatcgccg gcgccggaaa catctgaaat gatgcgtgga aaagcaggaa
    15901 tgataccgct ttcattaggt ccaaaacaaa acagcccttt ttcagataag gtaatgataa
    15961 cgagttggtt gtccaatttt tgctgaatgt atcgggcggc ctcgttcaac gattcctgtg
    16021 tggtttctat gcggctgggg tattgatttc tgatctcttt gagattgggt ttaaataagg
    16081 tgacaccccg gtatgcccag aaattcttta ctttaggatc aacggcggtg gggatgtttc
    16141 gctccatcgc cagttttgtc actgcttcga tgacttcggg agtgagtact cctttgttgt
    16201 aatcctgaaa aatgacaaca tctatcgggt ggttcttcaa tatctccgca atgccgctca
    16261 gcagcagtcc ggcttcttcg gtcgagatgt cttcggtcgt ttcttcatct attctcagca
    16321 ggtgttgggc gccggccatg atgcgcgttt ttaccgccgt ttttctgccg ggacaaccaa
    16381 taatgccttg agtaggaagc ccttcctggc gaatgagtgc cagcaattcc tcggctgctt
    16441 cgtcgttccc aactgcactg caaagccagg ctttggcgcc catagcctgc acgttgaggg
    16501 ccacattggc cgctcctccg agccgggcgt cttttgtgcg aagctgcacc accggcaccg
    16561 gggcttcggg cgaaatgcgc tccacgctgc cgatgaggta ccggtcggtc atcacatcgc
    16621 cgacgatgag cgcttgcagg cggtcaaatg cgagccagtc tatcatgtca gaagtatttg
    16681 taaattaatt acttagcatc tttttaaagc cacaatccac aagctactag ccttttcgtg
    16741 atgcaaaatt attattagtt ccttagccgt tccactaccg caatcgtcag ccggctggta
    16801 caaataagcc gctcttggtc atctacaatc tcgatgttcc aaacctgaat gagccgcccg
    16861 attctgacgg gccgggccgt tccccacaca tacccgtccg agacggagcg caggtggttg
    16921 gcattgattt ctacccctac cgacatatat ttggaggtgt cttcgatgca cagaaaagag
    16981 gcgaaactgc ccagcgattc agccagtaaa accgaagcgc cgccgtgcag caggcccaat
    17041 ggttgtttgg tacgatggtc aacgggcatt ttggcggtga tgaagtcggg gccgatttcg
    17101 gtgacttcta tgccgaggtg ttccatggcc gtattggctg caaatgaatt gacttgcgtt
    17161 aacgaaagat gatgtttcca gatcatttta aaatgtgctg ttgtactgat gacgtattac
    17221 aagccatcaa caaagtgaag aaaatcttcc cactgggcgg ggtggaaaac ccgccagaac
    17281 ccgtcgcgcc gaaaccaggt aagctgtctt ttggcgtaat tccggctgtg ctgcctgatg
    17341 agttgtacgg cttcatctaa attggttttg ttgtcaaaat aattaaatnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nncaattgta cataaaccgg ctgaaaactt ctcggctcca aaggttgatt
    17581 tctaaaagat gaaaacggcc ggccggcagc cctgcaaacg gcaacggcgc gcagcaaacg
    17641 ggccggattg ttggtgtcca cctcggcgaa atagtcgggg tcggcctgtt tcaactcgtc
    17701 ttgcagcgcg gcaatgcctt tcagatcgta catattttgc acttcagccc tgatagattc
    17761 cggcacttcc gggaagctgt tcagtccttc gcagactgcc ttgatgtaca atcccgaacc
    17821 gccggtcatc acggctacgt cgcgcgtttc aaaaatctga tggagacatg ccagggcctc
    17881 gcgctcaaaa tcgcctacgc tgtagggctg atgaatgctc aggtggctga tgaaatgatg
    17941 cggggcggcg gcaagttctt cctcggtcgg tttggccgtg ccgatgttca tttctttgta
    18001 aaactgccgg ctgtcggagg agagaatttc tgtttgaaag tgcagcgcgg cccgaatggc
    18061 aaaggccgtt tttcctgtgg cagtagcgcc tcctatgacg atgagatgtt ttgagtgcat
    18121 gtcaaatcct gatcgggcgc gaaattagac caatatcgca tatctttgcg catccacatc
    18181 caattttgcc tcgctaccga catgctgtac tacctcgttc gcccattggc caccattgcc
    18241 ctcaagattt ttttaagaaa aatcaccatc accaacatgg agcgggcgcc gtctgcccgn
    18301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac caccctacgg cattcatgga
    18361 gccgtgtatt ctggcctgca cgcttggtcg ccccttgtat tttttggtgc gcggcgatgt
    18421 gtttgccaag ccgttttatg cagccatttt gaaggcattt cacatgttgc ccatgtaccg
    18481 gctcaaagac cggggatata aatttgtaaa agaaaatttc caaaccctcg acgcctgctt
    18541 cgatgccctg cacgaaaaca aaaccatcat gattctggcg gaaggtacca cgatagcgga
    18601 aaaaaggctt cggcccatca aaaaaggtac tgcccggctg gctttcggcg cattggacaa
    18661 ataccctgac atggaggagg tgcatgtcgt tccggtaggg gtcaattttg actacatgga
    18721 tcggtttcgc gcagaggtga tgattgattt cgggcatcct atcaaggcca gagattacta
    18781 cgaaatgtac cgggcgaacg gttcggaagg aacnnnnnnn nnnnnnnnng gagttggccg
    18841 ggcgcatgcg gtcactgatt gtcaacgtag aaaaacaatc ggatgacgcc ctcgccgagc
    18901 aattattggt catgtcgcgt tcgctgcgca gattcacttt tttgccggta ttgggcaaag
    18961 accccgctct gttgcaacag gagcagttgg tggcaaacta tatcaacacg cttgccccaa
    19021 ctgaaaagtc ggcactcaca ggcgcctgcg ggcaatactt cgaggcattg caaaaaggag
    19081 gcgtttccga ccgggctgtg gcagagggga aaaacgacag cttgagtgaa aaactattgc
    19141 tccttgcagg tttaccggtt tttcttgcgg gttttgtttt caacttcctc ccggcccgat
    19201 tggcgcgcta cattgctgat aaacagatag aaagcgtgga gttttatgcc ccggtcatgc
    19261 tggtgcttgc tatggcggcc tacctgttgt actacgccgg ctgggggctg tttctgggct
    19321 ggaagggcct gtggctgcta ccgctcgcag cattggcggg ctatttttcc gtgctttaca
    19381 gagaggcttt tcagttgtgg aaatacagac gaagatgggc ggctttgccc gaagatcggc
    19441 agcgcagcct ccatgccttg agggccgatt gctcattgaa tgtgtagtga gcgcaactac
    19501 tccagcaaaa acgccagttc gtcgcgctgc aaactcagct tttcggtgtg ggcaataatg
    19561 tcctcggcga gctgcttttt gcgatcctga agcactaaga ttttttcttc aatgctgccg
    19621 cgggtgatga atttgagcgc aatcacgttt ttgtcctgcc cgatgcgatg ggcacgggca
    19681 atggcctgct gctccacaaa gggattccac caggggtcga gtacgaacac ataatcggcg
    19741 gcggtgagat tgaggccggt gccgcccgct ttgatggaaa tgagaaaagc ctgcacctgc
    19801 gggtcgttct gaaaacgcgc aatttcagcc tgcctttggg tgctgctgag gtctccgctg
    19861 agccaggcaa ggggcagccc tgcttcctga agggcttgtt tgaataagtc cagataactg
    19921 acgaaagaac tgaacatgag cactttatgc ccgctccggc gaatgacgtc ccattcctcc
    19981 agcacgtcct gaaacttgcc gctgccctgc cggtaatcat cgagaatcat gcgcggatgg
    20041 tttaccaatt gccgcagtcg catgcggggt tgatgcacca ccatttggta ttggccgttg
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgg
    20221 caggtctttg gccacctcct ctttggtgcg gcgcagcagg tacggttgca caagagtgcg
    20281 caggcggttt ttctgggcct cgtcctgttg cttttcgatg gggaggatga aggctttttt
    20341 gaaaaaagca tgcgtaccga gcagttcggg attgatgaat tgcatttgcg accacagatc
    20401 gctgagcgag ttttcgatgg gcgtaccgct gagggagact ttgtgccggg cagagaggct
    20461 gttgagtgtc ttaaatacct gactgtcttt gtttttgatc tgttgacttt cgtccaaaac
    20521 gatatactcc cactcggttt tgagcagcaa actgctgtcg cggagggcgg tttggtaggt
    20581 ggtgagtact acgtcgaagc gagccagcag ccggatgtct ttgtgacgcc ctgccccggt
    20641 atgcacatac acgctgaggc tgggcgcaaa ctgggccaac tccctttgcc agttgaaaac
    20701 caaagaggcg ggcagcacca ccaatgcctg caatgggcgc aaaaagtcaa catcggcggc
    20761 gggctgaaac aacgacatct ggccggcgga aggcgcttcg gctgcgggtt tttgagtctt
    20821 ggcatagagt aaaaccgcaa tggtttggag ggttttgccc aagcccatgt cgtcggcaag
    20881 acaggcgccc aaaccctcgc catgcaggcg cgccagccat tggaaaccct gcaattggta
    20941 ggggcgcagt tcggcgcgga ggccggcggg cagcggtgtt tcgttgttcg tcggtgcatt
    21001 ggacagcgcc tcctcgtcca tgagtccggc gtcttggagc agcccgatct ggtttttggc
    21061 aatgcggagt tgctcgccct gtttgcgccc catttgtgcc aggcctttgt agcggctcat
    21121 ccactcggca gggatgagga acacctcgcc gttggggagc ggatacaggc gattgccctc
    21181 gcggatgtac ggcgccaaca acagaaatgg gatggtatgc ggcccgatgc gcacctcccc
    21241 attgaggtcg aaccagtcgt tgccctgctg tgtattccac tctaaatgag cggtttctgt
    21301 cagtaattta ccctgctcgg tttcgggcgt cgttaccgtg aagccctcgg cttcgagttc
    21361 gcggcggtgt tcgcagagcc actggagcag ttgccacgaa tcttcggcag ggtggggtag
    21421 gacgaactga cttccgttgc cggcaataag acctaactgg cggagtgcct ccatatagca
    21481 ctcttcctca tcacggtggc gctgcacttt caggatgcgg atgtcgtcgc cgttgccgaa
    21541 ttcgagcgat gtgcgctcct gtcgggtatc tcggcaggca aactcggcgc cgggatacgc
    21601 catgtaggcg gttagtgccc attgcccgct gaaaagatca tgcacagcct ccaaacggca
    21661 ggttttcagt tgattgtgcc gtatgatttc aaaaccctcg gcctcgatat cggctttggc
    21721 ggctactttg aggatgaagc gctggaagta ggttttgacg ctcgacttcg ggatgcgcac
    21781 ctcgttctta ttgcgaaacg gcagcaccat gttgccgttg atgtgctcca cgcggtacag
    21841 acggtagtcg gcaaaaatcc aggcaggctg attgcacaca gaggccactt cccgcgattg
    21901 gatgttccag actttacccc cggcttcggc caatttcagc cggtaagaga cgccttccgg
    21961 caaacggctg aacgagaggc ggggctgtag cggcgcatcg gcaaattgca gcaccatgtc
    22021 tttgaccaaa ctccggcgct cggcatccca actcacagca aaaccgtact tctgcacaag
    22081 gctcaggagc tcgtctatgc ggcgctgaac gtattggtct atctgctttt tcagcttttc
    22141 atcttcgtac aattcggcca acgggcgggg ctttttggcc ttgccgttga agtattgttc
    22201 cagcgctttg gcctgtattt gctccacgat gcgcaacagc cggcggtgct gctccacctc
    22261 ttctactttg aaggaatcca gaatatgcag ggctgctttt tgtacgatgt gggtaagtgc
    22321 gccgtcgcgt tcctgttgta ccaaaaaagc gttcatgccg taaagtccgg gcaggagttc
    22381 gcagaggtta taaacgatgg tagtgcgggt attcggaaac ataaaatcag caggcgtcaa
    22441 tcaacagggg cgcaaagatg gggcaaaatg tgggacatcg cctatatacg gcaagatttt
    22501 atggcgtgcc cccgctcagc cggctccccg actttcagca gagacgggca gcggggtcgg
    22561 gtgtttcgct attatggcgc cttctatcgt cgggccatac cgcttcaccc cctcacgcgg
    22621 ctcggcgctt ccaacagaag cgggcttccc tccggccttg gcgccacaga gaagcccgct
    22681 tttactttaa ttgcggttga ccactacccg ccgcgtcgca tccggcagac cggcagattt
    22741 gactttcaat aagtacatcc cgctttggaa gcctttcaaa tcaaagcgct ccaccgtgtt
    22801 ttgcacttcg tccaattcag caaactgcag cagcttgcct tccaggctgt acgtttctat
    22861 gcgcacgctg cgcccggcgt actgcgtcag gtcgaggttc aactcgccgc ctgttgggtt
    22921 ggggtaaacc tcgaagctgt atggagtatc caggctcgca ccatgcaggg cgtggtcgaa
    22981 gctcgtagct gttgcgttac tacccgtata actcaccccc gcaaacgtag ccgtcaccgt
    23041 gctgttggcc gtgtaattgg tagccactaa gcccatttcg atgcagttgt tcatcgcgat
    23101 ggtctgtgcg ccaatgagga accaacttgt tccgttggcc gaaacagaca tggtaaactg
    23161 attgcccgca cgggtgatgc gcagccagta gcggttgttg ctcgccgatt gctgcggtat
    23221 tgcagcgccg ttggtcgttg tgcggaactc ccggcggtgc aggctgctca ggttggtcat
    23281 caactgcgcc tttttagcgc cgggggcatt gctttcgcgc atgacgatgc cggcccagcc
    23341 aagtgcgctg ccgctgatgc tggtcacctg tgcggtgagg ctgccgtnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnngtggca tccgaagtgt aaggcgggcc gtagaaacaa ttggtactcg
    23461 tggctgtcca tacgccggta cctgagttgt aggcgttgtt gttgcccgaa cagcctgcgc
    23521 cgccgggttg ttggctccag cctgccggaa atcctgaaac ggtaatgttt gaagtacagg
    23581 ttgccgtatt gccgttgatg tctgttgcgg tgacggtcac cggaacgatt tgtcctaccc
    23641 gctccgcaga gatggtattg ggcgacagac tgatgctctg cacgccgcag ttgtcggaga
    23701 cctcggccag atcatcggtg tcgagcgcga tgctttcctc accgttgaag ttcaatgtct
    23761 ggttgaagca ttgcaccatc ggcggcgtgt tgtccacttt ggtcaccgtg aaggtgcagg
    23821 agttggtcag gccgttgata tcggtcacgg tcagcgtcac gctcatgttg cccgcaccgg
    23881 agatggtggt tcccgcttcg ggagattgtg tcacgttttg cacgccgcag ccgtcgccgg
    23941 tggtggccaa gttagtgtag tcgggcagta cggccgtgca gttggcaccc agcgccaggg
    24001 tttgtgtggc ggggcaggtg atcgtcggtg cggtgttgtc cactttggtt accgtgaagg
    24061 tgcaggtatt ggtcaggccg ttgat
//
LOCUS       DAORLF010000017        23387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1204_length_23387_cov_2.267187, whole genome shotgun sequence.
ACCESSION   DAORLF010000017 DAORLF010000000
VERSION     DAORLF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23387
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_23387_cov_2.267187"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..>694)
                     /locus_tag="PK198_02830"
     CDS             complement(372..>694)
                     /locus_tag="PK198_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF37699.1"
                     /translation="WAHYNHPYTSNILNTPQEDSIAYRRSSPIYHAEGLRDDLLILHG
                     MVDSNVHFQDVARLSQRLIELGKDKWEMAVFPLEDHGFVTPSGWTDEYKRIWLLFERT
                     LKKK"
     assembly_gap    695..852
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<853..2742)
                     /locus_tag="PK198_02835"
     CDS             complement(<853..2742)
                     /locus_tag="PK198_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRF37700.1"
                     /translation="MKKSLLLPLLFLAGLAAAQIPANRSPLRIETIMQGERFAGFSPD
                     DFSWSEDGKTIYFTWNPELDTLRSLYKTDLNGSAPQRVSIEEQQNMVSGGAYSRDRSR
                     KVYSKNGDIFLWEAATGSSRPLIQTTASEFAPRFSGDERQVVYQSGDNLFSWDMSTGA
                     IRQLTNFQRGAKREDPRKSSQEQWLETDQLALFDVLRERKAESETRERRTKALAPKRP
                     FEYYYGERSLSNLQISPDLRFVTFRLTTRAKTRTAQMPDYVTQSGFSSMQDTRTKVGA
                     AQDTHETGIFDAQRDTIYWLNTKNIEGIFDKPEFLRDYHRDTLPYNSKYDKPREIIMH
                     GPVFSDDGKAAVVLRSQDNKDRWIMLLDLPTGQLRLLDRQRDEAWIGGPGIGWGTGNI
                     GWLPDDRSLWFQSEATGFSHLYIIDVETGQRRALTQGKFEIISAELSRNQDYFYITAN
                     AEGPHEHHFYRLPVSGGKLEKITSLSGGNRAVLSPDERHIAVRYSTGNQPWELYLMEN
                     RTGAPMRRLTQSTTAEFQKYAWRMPEIVWFTARDGVQVPARLYRPAKAAKGGPAVVFV
                     HGAGYLQNVHRWWSTYSREFMFHNFLADNGYTVLDIDFRASEGYGRDWRTAIYRHMGG
                     KDLSDQ"
     gene            complement(3043..3486)
                     /locus_tag="PK198_02840"
     CDS             complement(3043..3486)
                     /locus_tag="PK198_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF37701.1"
                     /translation="MKKITKIKFTFLLFTTALMTACAQVPPADTLDYTAHPDIWPVFK
                     GCETLDNPDERNACSRREMLQYIYVHFKMPDVKDVHPCFTMTATFIIEKDGSVNDAAI
                     KKELHPLMDSELLRVINSMPRWTPAMTNGRPVRFKMSLPLRIRLE"
     gene            complement(3579..4031)
                     /locus_tag="PK198_02845"
     CDS             complement(3579..4031)
                     /locus_tag="PK198_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF37702.1"
                     /translation="MCCIAPVFSGAVWTTKLLFSPLASPPPPLQDETEICFRNPEFWP
                     IFNGCETLADYGEQRNCSKKKTIEYIYQHYKYPAIGDACVQGTIVVLCTIETDGSVSN
                     VRTVRGLHPSFDAEAERVIASMPRWTPGRMYDKVVRVEMIFPVRIRLE"
     gene            4100..4840
                     /locus_tag="PK198_02850"
     CDS             4100..4840
                     /locus_tag="PK198_02850"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HRF37703.1"
                     /translation="MKNQVFLIALKWAVINFILGWAISFASRPLMNLIPEYGVQMINF
                     IFMAIHSILTIAIAVLALRELEQKAGTEMSAGKAFELMGYMLLILALAGGTVMLVQYQ
                     FLFREWGLNSMMTSWVVSLGQRILFLLIAVGMYGVWRTFTRAGKPGWACLVPVYNQIV
                     QCEIGRKPAWWVVLLFIPVVNIVVSIMIINGISKAFGKDEGFTAGLVLLPFIFYPMLG
                     FGKDRYLYGPEPAEDRSIESMIEDHLLE"
     gene            5155..>5505
                     /gene="ruvX"
                     /locus_tag="PK198_02855"
     CDS             5155..>5505
                     /gene="ruvX"
                     /locus_tag="PK198_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRF37704.1"
                     /translation="MARIIGIDYGTKRTGIAVSDPLQIIASGLDTIPTHQVLDFLTRY
                     MAEEEVEIIVVGEPKYPDGSPAQIAHLVVGFVRQLQRAFPQVKIAMQDERFTSEEAKD
                     VIRQVGVGKQKRRDK"
     assembly_gap    5506..5515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5644..6201
                     /gene="def"
                     /locus_tag="PK198_02860"
     CDS             5644..6201
                     /gene="def"
                     /locus_tag="PK198_02860"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRF37705.1"
                     /translation="MILPIFAYGQPVLKKMAAPITPDYPELSTLIANMWETMYNAHGV
                     GLAAPQIGLSIRLFVIDTLQMKDEGEANDGFKKVFINAEMLDETGQAWAYEEGCLSIP
                     DIRGDVERQPDIRLRYWDENFQPFEETFTGINARVIQHEYDHIDGILFTERLKPLKKR
                     LVQRKLDAIRQGKVSSDYRMKFAIR"
     gene            6301..6423
                     /locus_tag="PK198_02865"
     CDS             6301..6423
                     /locus_tag="PK198_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRF37706.1"
                     /translation="MGKGDRRSTKGKTWRSSYGKSRPKKKNVKKVEPAKPEAEA"
     gene            6732..>8913
                     /locus_tag="PK198_02870"
     CDS             6732..>8913
                     /locus_tag="PK198_02870"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37707.1"
                     /translation="MHYAKHLCWVLCTFACTLFAQTQSRCVLEGRVADEKGMPVELAT
                     VVLKTAKDSVVIEAGYTEKDGSFRFANLAAGNYLVEVVFVGFEKHLGQVVLAGGSNSP
                     VLYPPIQLNPAQQQLSEVTVTARRPYVERLADRTIINPDVLIANAGSSALEALERAPG
                     VQVGEGGDIRLRGRSGVAIFVDDKPTYLSGTELESYLRSIPADNVKHIELMPNPPAKY
                     EAAGNSGVINIVTKRNRIAGFNGNTSLAFAQGRFNRSNNSLNLNLNRKNFGLFVNLGA
                     GHRNFFQDLNIFRFYTRMDGDPSSNFAQNSYIVPRSQSLNAKLGLDLYLSDKTTLGLV
                     VKGLTSPNERQTDNTAIVRAPFGAILNTVIADNAEKSSFANGAYNLNLRHRLDSLGSN
                     IIVDADYVTYRTDADQFFQNFIYDSSGTLGYADTIQGRLPSYITIYAAKADYTKPFAH
                     GLKMEAGLKSAFTQTDNEAAYTTTRVGQTYINYDLSNRFLYDEWIHAGYLNFSKTFGR
                     LDLQAGLRVEHTSLKGNQLGNEFQPGSKFSRNYTQPFPTLYANLRLDSLDKNTLSFSY
                     GRRIDRPFFKDLNPFISPLDKFTFYTGNPNLLPTFAHNLSLTHSFQGKVNTTLSYSKT
                     LDGINETLEIVNEIYYSRPGNIANSQSIGLSLEATLPVAKWWTLMLYAAADRVTYKSD
                     LYTQRLDATGYNGYISMTNSLQLGKGWSGEVRADYQTNQVYAQLL"
     assembly_gap    8914..8923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8924..9195
                     /locus_tag="PK198_02875"
     CDS             <8924..9195
                     /locus_tag="PK198_02875"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37708.1"
                     /translation="LNFGTLNLAAAKKIWKDAATIKLSAQDLLYTRRGSGIINNLELT
                     EANWNSRFDSRVLNLSFSYRFGKVANQRQRHNSSGSESEQQRVRS"
     gene            9287..>10012
                     /locus_tag="PK198_02880"
     CDS             9287..>10012
                     /locus_tag="PK198_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRF37709.1"
                     /translation="METVEKYVDIITEMLIKYTPKVLFALVLLWIGFRVINRLVDTGL
                     KAMERSGISQDVRPFLASLMGLLLKALLVFSAAGIVGIETTSFVAALAAAGFAVGLAL
                     QGSLGNFAAGIIILIFKPYKAGDLVSIGDNTGHVAEVQIFNTVLATLDNKTVIVPNSE
                     AINGAITNLSTRQILRVDLEVPMPYAEDFDRIEAILLEALRQTPKVLQDPAPFVGIQK
                     FDSHSIILDVRPYADAEDYWEVYY"
     assembly_gap    10013..10144
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10154..>10496
                     /locus_tag="PK198_02885"
     CDS             10154..>10496
                     /locus_tag="PK198_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRF37710.1"
                     /translation="MQVQTFTDQMGRQVEIPFPPQRIVSLVPSQTELLFDLGLGERAV
                     GITKFCVHPQVWFRSKTRVGGTKQLRMETIAALRPDLIIGNKEENERLQIEQLAAHYP
                     VWMSDVRDLSSA"
     assembly_gap    10497..10517
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10518..10965
                     /locus_tag="PK198_02890"
     CDS             <10518..10965
                     /locus_tag="PK198_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRF37711.1"
                     /translation="LGRMTGKATEAISIAENIQSGFDDLAVAVAQQPRLRAAYFIWRE
                     PWMAAGSDTFIDEMLRWAGFENAFAHLPRYPELSLEQLAAAEPDCVLLSSEPYPFREK
                     HLLEISRLCSKAAIRLVDGELFSWYGSRLAQSPAYLAALREARCRK"
     gene            complement(10975..11532)
                     /locus_tag="PK198_02895"
     CDS             complement(10975..11532)
                     /locus_tag="PK198_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37712.1"
                     /translation="MRPSVIFLAAMACLLCSPDLSAQAEIPSLKYGLKSRKKCTAFPN
                     DSYGYALLANPKEAYPVKNYPVRKSYELQFHCLGFSYESSEYLFIRFGDWSEKLTVPA
                     SGIRPVFVWKNVTLLGDEKRYLVLSYGEESDLMFTAFRVFDETGNDCLHPDHPDMRAI
                     VERLMKDLKKNNSSDEFMKKFRSVR"
     gene            11603..13198
                     /gene="treF"
                     /locus_tag="PK198_02900"
     CDS             11603..13198
                     /gene="treF"
                     /locus_tag="PK198_02900"
                     /inference="COORDINATES: protein motif:HMM:NF009774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HRF37713.1"
                     /translation="MRTNLFPITLFALALAAFSCRTETEKTAQASPDRRSPDEIYGEL
                     FHAVQTSSVFPDGKTFVDCSPKFPADTIMARYTAARNAADFDLKAFVAEHFDIPQSGA
                     SDFVSNPKHSPAEHINALWEVLTRRPDQAQAGTLIPLPHPFIVPGGRFREVYYWDSYF
                     TLLGLQDAGQKEMVRHIADNFAYLIDTIGFVPNGNRTYYLGRSQPPFFSMIVRIVEEM
                     EGQEAALKYLPQLVREYDFWMEGQKRLSEQNPTHRRVVRLADGIVLNRHWDDQPRPRP
                     ESYREDLETAQNNARESQAIYTDLRAGAESGWDFSSRWLEDPMDLATIRTTDIIPVDL
                     NALLFHLESIIAEYARLAGKTDQAARFEQLAAERKAHIVRYCWDETSGFFYDYDFENK
                     RHTGVPSLAGMYPLFFKVADQKQAEACAKVIQRDFLKPGGVVTTLTQTGQQWDAPNGW
                     APLQWITIQGLRQYGQTALADDIRNRWVALNEKVYRNTGKMVEKYNVMDMTLDAGGGE
                     YPVQDGFGWTNGVLLRLLTEEKK"
     gene            complement(13220..>13541)
                     /locus_tag="PK198_02905"
     CDS             complement(13220..>13541)
                     /locus_tag="PK198_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37714.1"
                     /translation="LSPEALFSFYDKGKFVYSLVNEATVAGKVVQEIEFKPLDKGSEY
                     AKFRLTVDKAAVTVVSMKAFGKDASRYTVKMGALTPNKTFAADFFSFSKSKFPGYRVE
                     DLRD"
     assembly_gap    13542..13563
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13564..13931)
                     /locus_tag="PK198_02910"
     CDS             complement(<13564..13931)
                     /locus_tag="PK198_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37715.1"
                     /translation="MTKKIWMLVIALFCVLSVHAQKQTGQFTKAQESDPQAKAVLDKL
                     KKKYQGYQSLELAFTLEIELPDQKKEVQQGKIAKQGKKYRVDLASQSVICDGTALWLV
                     LHNNKEVQINNLPDPSTDEN"
     gene            complement(14136..15233)
                     /locus_tag="PK198_02915"
     CDS             complement(14136..15233)
                     /locus_tag="PK198_02915"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRF37716.1"
                     /translation="MRANIIKFSKPETGDFFTTLNRRVNDYFVENKISRHANAAMIFK
                     TIFMLLLYFVPVVLIITATVTSPWMVFAMWCLAGLGVSGIGLSVMHDANHGAYSRKQS
                     INNMLGYTLNFLGGNMMNWKIQHNVLHHSFTNVDGYDEDIEPRKALRFSPHHPWMKHH
                     RYQHIYAWPLYALMTIMWITIKEFKQINRWKEEGIIEAQGRTYGGLLAELIVSKSVYY
                     VYALVIPLLVSPVAWWVTVLGFIAMHLISGLILALVFQPAHVVPEADFPLAPEDGNME
                     SSWAIHQLHTTCNFAPRNRILSWFVGGLNHQIEHHLFPNICHIHYKKLSPIVKQTAKE
                     FGLPYHEMPTFRSALYHHGQQLYNMGHLQPA"
     gene            complement(15326..15874)
                     /locus_tag="PK198_02920"
     CDS             complement(15326..15874)
                     /locus_tag="PK198_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37717.1"
                     /translation="MLYKVKLKNAAEEVILDDRVYEWLISDSYLSKLDFINNLRMHSS
                     GCAVFQKTWKKADGSFKTETIYLHKLVAEKFLAHTKTQECNLVGARNNNKLDCRVENL
                     IYRSRSVASRKRKTSGHMGYTGVYKENNRYRAVISINRKSVHLGMFSTPEEAALAYNK
                     KSREVYGEDGKQNVIKPKKEGE"
     gene            16098..>16544
                     /locus_tag="PK198_02925"
     CDS             16098..>16544
                     /locus_tag="PK198_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRF37718.1"
                     /translation="MTLHETYMQRCFDLAALGEGHTAPNPMVGAVLVYEGRIIGEGFH
                     AEYGGPHAEVNAVASVPASLRHLIPASTLYVSLEPCCITGKTGACTSLILKEGIKKVV
                     VSCLDMTPGVAGQGVALLKAAGVEVELGLLEPQGHFLSRVRNHFVTR"
     assembly_gap    16545..16554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16555..17146
                     /locus_tag="PK198_02930"
     CDS             <16555..17146
                     /locus_tag="PK198_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRF37719.1"
                     /translation="PYVMVKYAATPAGCFAPQPAARAWITHPYTQRLTHRLRSATAAI
                     LVGTQTALLDDPGLDNRLFYGPSPLRVVLDRRLRLPGHLKIFDGSLPTLIVHESEAGA
                     KTHRAAYLQLPFDADLLNNLLAALYERKISSLLVEGGAQLIQSFFDAGLWQEAIVYTG
                     SREFEHGLPAPRVPGVCVQTEQIGTDSLRRFVPHSC"
     gene            17238..17801
                     /locus_tag="PK198_02935"
     CDS             17238..17801
                     /locus_tag="PK198_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRF37720.1"
                     /translation="MSRYSLIFSLAVGALLFSMTEASAQNAAPAASAPAVQWFSWKEG
                     ITKATRENKKILLYVYTPSCVWCRKMERETFADETVAQLVKADFVAIKLNASDNAELE
                     FKGKTYRFVNSETGGCNGLAAELLGGRMSFPAVAFLDETQQALQSLVGFKSAEDFGAI
                     ATYYGKNYYQTMPWSSFQKKYAAGGGR"
     gene            complement(17809..18759)
                     /locus_tag="PK198_02940"
     CDS             complement(17809..18759)
                     /locus_tag="PK198_02940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF37721.1"
                     /translation="MRDKEKEIAAMSDEQARLELLYSRQRNVLDSLSMRGLRDSMALA
                     LSQAQTQEQAAELATERNRRNLLSALALLILVVAGGVYARYRGVSRYNTLLTIKNEQI
                     TAAQKRSEELLLNILPAAIASELKQSGAAQARQYEQATVLFTDFVGFSRIAKAVSAEQ
                     LVADLNYCYGQFDKITEKHGLEKIKTIGDSYMCAGGLPQSDTDHALRMVAAALEMQVF
                     LKMWNAQRAERGEAPLEARIGIHTGPLVAGVVGYRKFAYDIWGDTVNIASRVESAGEG
                     GRVNVSESTFQLVKSEFSCTFRGVLPVKNLPPVAMYYVES"
     gene            complement(18928..19206)
                     /locus_tag="PK198_02945"
     CDS             complement(18928..19206)
                     /locus_tag="PK198_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37722.1"
                     /translation="MLPLKYRLLIAILMSASIACMGQSKRITELERKLETQTGTELIQ
                     TKITLANLYLDKDNYDKALEHAQQAQSLAEAGNHKDWVRRSLTVQGKI"
     gene            complement(19257..20273)
                     /locus_tag="PK198_02950"
     CDS             complement(19257..20273)
                     /locus_tag="PK198_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37723.1"
                     /translation="MKHFYILALGLTIFALPACHRDAHEPLPAFYHWKTQLSLSSEER
                     ELLSGMKARRLYVRFFDVDWDEAYDVPAPLAELKIDTARLEGLEIVPCIFITNRCMEK
                     LPAEGTAELAQRIWQKTETLAAQYPGLRFSEFQADCDWTAGTRERYFVLLESLRKILN
                     DKNIALSVTVRLHQFSSPETTGVPPADRGMLMCYNTGDLENWDEENSILRAEEAALYF
                     KNAPPYPLPLDGALPVFSWGVLFRNDQMIRLIHDLQPEQLADTTRFVCTAPHRYKVVK
                     STWMEGHYLYAGDRLRLELPDPEDVKQCAQWLKQAMQRSSDQSVLAFYHLDAGALRYF
                     KDSK"
     gene            20445..20876
                     /locus_tag="PK198_02955"
     CDS             20445..20876
                     /locus_tag="PK198_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HRF37724.1"
                     /translation="MNEFLPELTAAFNEKGETISPKLPEWVKNFLIDIDGTICDDVPN
                     EEPERMGTCLPYPDALKYVNKWYDEGHIITFFTSRTEEHREITEEWLDRHGFKYHGVL
                     YGKPRGGNYHWIDNHIVKATPFKGKFTDLVVQTAEIEVFAG"
     assembly_gap    20999..21142
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21143..22547)
                     /locus_tag="PK198_02960"
     CDS             complement(<21143..22547)
                     /locus_tag="PK198_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF37725.1"
                     /translation="MPALLRSVLMFARLALPVAMVYFMAGCASISPLEGGPKDETPPK
                     VLEEESTPNFQANSSPRRIELVFDEWVTLTDVFNQVVISPPLQYKPAVSLKGRTVVFE
                     LNEKEQLRPDATYTINFGNAVKDLTENNPAENLRFVFSTGAYIDSLTVSGKIVSDLTG
                     EPVDKALFMLYDNLNDSVVRTELPFYFGRTDKNGHFTIPNVRADTLKGFALLDADLNY
                     RFNQSKELIGFPDAWIITTDSSARDLRIRLFEEARPIKLLEQETATYGVVKLTYSQTP
                     LSTNFSASDTAQIWKTEYLGDSIKIWYDRPDTNSWKLFVRADTITTDTITPKNGSLKP
                     TFLSKAKLSMVSPTPGATALLHPDKPAALTFNHPVVRADTSLISWWEDTLRNPVFPTW
                     KPDSVQPRVWNVSLPWKEALIYEMQALPGAFTDMYGLSNADTLLLKWRIEQRKNFATL
                     HIIVEDMEPETDYIVSLL"
     gene            complement(22553..23287)
                     /locus_tag="PK198_02965"
     CDS             complement(22553..23287)
                     /locus_tag="PK198_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37726.1"
                     /translation="MSFYLNGERQQVSLQASRIDLTNDGDLIIGNSDCLGANEAPFVG
                     LIDEFRVYNRGLDERDILDLYFGPDRILSRDTLIYLGETVSMELAQTCATQFSWSPQS
                     GVANPTAGETDITTPLAGVFNYALSMGDGSSSCIATDSIRITVVDRNELDCNSLFLPS
                     AFSPNNDGLNDNFGLSNPFAATDLISFEIFDRWGGRVFYTENAFEKWDGSFNGTPVNP
                     GVMLYRIRYRCNGEEQSKTGSVTILR"
BASE COUNT         5337 a         5911 c         6100 g         5532 t
ORIGIN      
        1 gtttgtttac tcgttgaaaa attagatttt ccatttgggg aaatttcaaa atctcatctt
       61 aaaaaaaggc cctttttact ttgggaaatc aaacctgact accaatcagg caggctcgtg
      121 actcgcggct cgcagctttt tgcagggcga ctgaatagtc acgtcagata aacgcttcat
      181 tttaaaggtc ttaaccaatc ctgttgcctc aaaaaaatga caatttgttc ttgccccgct
      241 gcaaaggtaa agaatgcgtt cgctctacgg gcgggttttt acagtttagt gaaggggtga
      301 ctcagctaag atcacagccc gtagtcattc cttcactacg catagacacc aaactcagcc
      361 tgcccggttt cctacttttt cttcaaggtt cgttcaaaca gcaaccatat ccgcttgtac
      421 tcgtcggtcc agccgctggg cgtgacgaaa ccgtgatctt cgagcggaaa aacggccatt
      481 tcccatttgt ctttgcccaa ttcgatgagg cgctggctga ggcgggccac atcctggaaa
      541 tgcacatttg agtccaccat gccgtgcaga atgagcaggt catcgcgcag gccctcggcg
      601 tgataaatgg gcgaagagcg gcggtaggca atgctgtctt cctgtggcgt gttgaggatg
      661 ttggaagtgt agggatggtt gtaatgcgcc caatnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnctgatcgc tgaggtcttt gccgcccatg tgccggtaga tggccgtgcg
      901 ccagtcgcgg ccatatcctt cgctggcgcg aaaatcaatg tcgagtacgg tatatccgtt
      961 gtcggcgagg aagttgtgaa acatgaactc ccgcgagtag gtactccacc agcggtgtac
     1021 gttttgtagg tagccggcgc catgcacaaa gacgacagcc ggaccacctt tggctgcttt
     1081 ggccggacga tagagacggg caggtacctg tacgccgtcg cgggcggtga accacacgat
     1141 ttcgggcatg cgccaggcat atttttgaaa ctcggcggta gtggactgcg tgaggcggcg
     1201 catgggcgct ccggtccggt tttccatgag gtacagttcc cagggctggt tgccggtgga
     1261 gtatctgaca gcgatgtggc gctcgtcggg cgagagcacg gcgcggttgc cgccggagag
     1321 gctggtgatt ttttcgagct tgccgccact tacaggaagg cggtaaaaat gatgctcgtg
     1381 cgggccctct gcattggccg tgatgtaaaa gtagtcctga ttacgcgaaa gctcggcgct
     1441 gatgatctcg aatttgccct gcgtgagggc gcggcgttgg ccggtttcta catctattat
     1501 ataaaggtgt gaaaaaccgg tggcttcaga ctggaaccag aggctgcggt cgtcgggcag
     1561 ccagccgatg ttgccggttc cccagccaat gccggggccg ccgatccagg cttcgtcgcg
     1621 ttggcggtcc aagaggcgca attggccggt gggtaagtcg aggagcatga tccagcggtc
     1681 tttgttgtct tggctgcgca acaccacggc ggcttttcca tcgtcggaga agaccgggcc
     1741 gtgcatgatg atctcgcggg gcttgtcgta tttgctgttg taaggcaggg tatcgcggtg
     1801 gtaatcgcgc aggaattcgg gcttgtcaaa aatgccctct atatttttgg tgttcagcca
     1861 gtaaatggtg tcgcgttggg cgtcgaaaat gcctgtttcg tgggtgtcct gtgcggcgcc
     1921 tactttggtg cgggtgtcct gcatggaaga aaaaccggac tgtgtgacgt agtcgggcat
     1981 ttgcgccgtg cgggttttgg cgcgggtagt gaggcggaag gtaacgaaac ggaggtcggg
     2041 gctgatttgg agattggaga ggctgcgttc gccgtagtaa tattcgaacg ggcgcttggg
     2101 agctaaggct ttagtgcggc gttcgcgggt ttcggattct gctttgcgtt cgcggagcac
     2161 gtcgaacagt gccaactggt cggtttccaa ccactgttcc tgcgaggatt tgcgggggtc
     2221 ttcgcgtttg gcgccgcgct gaaaattggt gagttgccgg atggcgccgg tgctcatatc
     2281 ccaggaaaac aggttgtcgc ccgattggta aaccacctgc cgttcatcgc ccgaaaagcg
     2341 gggggcaaat tccgaggccg tcgtttgaat gagcggccgg ctgcttccgg tggctgcttc
     2401 ccacaaaaaa atgtctccgt ttttggaata gaccttgcgc gagcggtcgc gggaatacgc
     2461 tccgccggaa accatgtttt gctgctcttc gatggagacg cgctgcgggg cgctgccgtt
     2521 caggtcggtt ttataaagac tgcggagggt gtccagctcc gggttccagg tgaaatagat
     2581 ggttttgccg tcttcgctcc atgaaaaatc gtcgggcgaa aaaccggcga agcgctcgcc
     2641 ctgcatgatg gtttcgatgc ggagcgggga gcggttggcg ggtatttgcg cggctgcaag
     2701 gccggcaaga aagagcaagg gcaaaaggag tgattttttc atggcgcggg tgattttttt
     2761 tgaatgagcg actgaaaccg cagggtgctt gtgtccctta ggggcgataa aaaataaagt
     2821 ggtcattttc gccaaaggat tagataagta aacgtttcat tttaagtgtc ttatctaatc
     2881 ctttggcata gagaaaatga acatttattt ttgccccact acttatgaca gcgcgaaagt
     2941 acagaaaaag ggagaattta gtgtggggcc ggttgatttg gcgcaagccg cgagttgtag
     3001 tatccgttca taaaatcact ctttggggga gggttgcaag catcactcca atcggatgcg
     3061 taagggcagg ctcattttga atctcacggg gcggccgttc gtcattgcgg gcgtccagcg
     3121 gggcatggag ttgatgacac gcagcaattc ggagtccatc aaaggatgca gttccttctt
     3181 gatcgccgca tcgtttaccg aaccgtcttt ttctatgatg aaggtggccg tcatggtaaa
     3241 gcagggatgc acatctttca catccggcat tttgaaatga acatagatgt attgcagcat
     3301 ttccctccga ctacaagcat tccgctcatc aggattgtcc aaagtctcgc agcctttaaa
     3361 aacgggccag atgtcgggat gggcagtata atccaacgta tcggccggcg gaacttgcgc
     3421 gcaggcggtc atcagagctg ttgtgaacag tagaaaagta aactttattt tcgttatttt
     3481 tttcatatta aagtgctgta aatcagtgat ataatataaa aatcgctacg ctggcgcaag
     3541 attccatctt gtacaccccc tgcgcgctca cgtccgtctc actccaatcg gatgcgcacc
     3601 ggaaatatca tttccaccct gaccaccttg tcgtacattc ttcctggcgt ccagcggggc
     3661 atggaggcaa tgacgcgctc ggcctcggca tcgaaggaag ggtgcaggcc gcgaaccgta
     3721 cgcacattac tcacagagcc atctgtttct atggtacaaa gaacaacgat tgtaccttgt
     3781 acgcatgcat ctccgatagc cgggtatttg tagtgttggt agatgtactc aatcgttttt
     3841 tttttactac aatttctttg ctcgccgtag tcggcgagcg tctcacagcc attaaaaatg
     3901 ggccaaaatt caggattgcg aaagcaaatc tccgtttcgt cttgcagcgg cggcggcggt
     3961 gaagcaagag gtgaaaaaag cagtttcgtc gtccagacag caccgctaaa aactggcgca
     4021 atgcagcaca aaaaaattat tatcttgctc aatgaaaatt attgtctcat cttggcggct
     4081 tattgccaaa tattttgcca tgaaaaacca agtctttctg atcgcgctca aatgggccgt
     4141 catcaatttt atactgggct gggccatcag ttttgcaagt cgcccactga tgaatcttat
     4201 acctgaatat ggggtacaaa tgattaattt tatattcatg gccattcatt ccatattgac
     4261 aatcgcgata gcagttctgg cattgcggga attagagcag aaagcaggta cggaaatgag
     4321 cgcaggcaag gcttttgaac tcatggggta catgttgttg attctggccc tcgccggagg
     4381 taccgtcatg ctggtgcaat accagttttt gttccgggaa tggggactca acagtatgat
     4441 gacgagttgg gttgtcagtc tgggccaacg catattgttt ttgctcattg ccgttggcat
     4501 gtatggcgta tggcgaacat tcacgcgcgc cggcaagccg ggttgggcct gcctcgttcc
     4561 tgtttacaat caaatagtac agtgcgaaat cgggcgaaaa ccggcatggt gggtggtttt
     4621 gctctttatt ccggttgtca atatcgttgt atctattatg attatcaatg gcatatcgaa
     4681 ggcgttcggg aaagacgagg gcttcacggc gggcttggtt ttgttgccgt ttatttttta
     4741 ccccatgctt ggcttcggca aagaccgcta tctctacggc ccggaaccgg cagaagaccg
     4801 ctccatcgaa tccatgatag aagatcattt attggagtga gctgcctgtc ggggcgaaat
     4861 tttacagaaa aaacaaaaaa aattcaactt gcgtgtgacc tgccccgcaa gctcgcgtct
     4921 caaagccgtt aacaagctga aagccgggcg gcagcgcccc aaaaaaccga aatcatacat
     4981 cctgaaatcc ccttctgctc ccaacgggga gcgcaccaca ccgcctcatt gcgaggatac
     5041 catgagaaat aacacgcttt caaaacggag gccaacaaga gtataagtaa ggggaacggt
     5101 tgtcggggcc ggcctttggg tcggaatccc ggcaactttt tttatttttg cgccgtggca
     5161 agaatcatcg gcatagacta cggcaccaag cgcaccggca tcgcagtgag cgacccgctc
     5221 cagattatcg caagcggatt ggataccatt cctacccatc aggtattaga ttttctcacc
     5281 cggtacatgg ccgaagaaga agtagaaatc atcgtggtgg gcgaacccaa atacccggac
     5341 ggcagcccgg cccagatagc gcatttggtg gtgggcttcg tcaggcagtt gcagcgggct
     5401 tttccgcagg tcaagatagc gatgcaagac gagcgattta cttccgaaga agccaaagat
     5461 gtcattcgtc aggtaggtgt cggcaaacaa aagcgccgcg ataaannnnn nnnnncgcca
     5521 ccatcatttt acaaaatttt atggaaaagc accggtattc actcaacaga taaaccggtt
     5581 tgcgcgttgt atcaagctgc ctgaaacggt tgtcttttca ggcattttca agatttgtta
     5641 cttatgattt tacctatttt cgcctacggg caacccgttc tcaaaaaaat ggccgctccc
     5701 attacccccg actatccgga gctttccacc ctcattgcca acatgtggga aaccatgtac
     5761 aatgcgcatg gcgtgggcct ggcggcgccg caaatcgggc tgtctattcg tctcttcgtc
     5821 attgacaccc ttcagatgaa agacgaggga gaagccaatg atgggttcaa aaaagtattc
     5881 atcaacgccg aaatgctcga cgaaacaggc caagcctggg cttacgaaga aggctgcctg
     5941 agcataccgg atatccgtgg cgatgtggag cgtcaaccgg atatcaggct gaggtattgg
     6001 gatgaaaatt tccagccctt tgaagaaaca tttaccggca tcaatgcgcg tgtgattcag
     6061 cacgagtacg atcatattga cggcatcttg tttaccgagc ggctcaaacc attgaaaaaa
     6121 cgccttgtac agcgcaagtt ggacgccatt cggcaaggca aggtcagttc ggactatcgc
     6181 atgaaatttg ccatccgata gtttttctcc agtgtattct gccgccatta aagaccaagt
     6241 attatctttg gcggctcgaa ttatccatct ttattcagat caaccaataa tttaatcatc
     6301 atgggcaaag gagatcgcag atccaccaaa ggaaaaacct ggcgcagcag ttacggcaaa
     6361 tcgcgcccca agaagaaaaa cgtaaagaaa gtggaaccgg ccaaacccga agcggaggct
     6421 tagtttcttt gttatttctt cgcccgacca cgccggcaca gcgctaccct gctgtccggc
     6481 gttgttgttt ccggcggtat tcgatcctca gacaacaact tcggcggtaa tttaccagag
     6541 taatgaggtg ttcttgcatg gataagaaac acaggagagc aggctattac cctgacaacg
     6601 tgtcttttgc tacttggtct aatttttcag tgtgttggta ggataatcgg atgtaatacg
     6661 ttgtgtttct acgaatccat tcgtaggttt gatggcgtta tttcatccat agaccaaacg
     6721 caaaatcacc tatgcactac gccaaacacc tctgttgggt actctgtact ttcgcctgca
     6781 ccctatttgc acaaactcaa agccgctgcg ttctggaggg gcgtgtcgcc gatgaaaaag
     6841 gtatgcccgt agaacttgct actgtagtat taaaaacagc gaaggattcc gtcgttatag
     6901 aggccggcta taccgagaaa gacggtagtt ttaggtttgc caatctggca gccggcaact
     6961 accttgtaga agtggttttt gtaggatttg aaaaacattt ggggcaagtc gtacttgccg
     7021 gcggaagtaa tagccctgtt ctgtaccctc caatccaact aaatcctgcg caacagcaat
     7081 tgtcggaagt gaccgtaacg gcccgcagac cttatgtaga gcgcctggca gatcgcacca
     7141 tcatcaaccc tgacgtactt attgccaatg cgggcagcag tgcgctggaa gccctcgaac
     7201 gggcgccggg cgtgcaagtc ggcgagggtg gcgacatacg gctgcggggg cgttcgggcg
     7261 tggccatatt tgttgacgac aagccgacct acctcagcgg gacggagttg gagagctatt
     7321 tgcgttccat tcctgccgac aatgtgaagc acattgagtt gatgcccaac ccgccggcca
     7381 aatacgaggc tgcgggcaat tcaggtgtca tcaacattgt gaccaaacgc aaccggatag
     7441 caggtttcaa cggcaacacc tcgctcgcgt tcgcgcaggg ccgctttaac cggagcaaca
     7501 acagtctgaa cctcaatctg aatcgcaaaa atttcggcct gtttgtcaat ctgggcgccg
     7561 gccaccgcaa ttttttccag gacctcaaca tcttccgttt ttacacccga atggatggag
     7621 acccttcttc caattttgcc caaaactcct acatcgtgcc gaggtcgcag tcgctgaatg
     7681 ccaagttggg cctcgatctg tatttgagtg ataaaacgac gctgggcctc gtcgtaaaag
     7741 gactgacatc acccaatgaa cgacaaaccg acaatactgc cattgtgcgg gcgcctttcg
     7801 gcgccatcct caataccgtc attgccgata atgctgaaaa atccagcttt gccaacggcg
     7861 cctacaacct caatcttcgc caccgcctcg attctctggg cagcaacatc atagtggatg
     7921 ccgactatgt aacctaccgc accgacgccg atcagttttt ccaaaacttc atttatgaca
     7981 gctccggtac actcggatac gcagatacga ttcagggccg gcttccttct tacatcacta
     8041 tttacgcagc aaaagcagac tataccaaac cttttgctca tggcctgaaa atggaggcgg
     8101 ggctaaaatc ggccttcacc caaaccgaca acgaagcggc ttataccact acccgggtcg
     8161 gtcagaccta tatcaactac gatctgagca accgctttct ttacgacgaa tggattcatg
     8221 cgggctatct gaatttcagc aaaacctttg gcaggctcga tcttcaggca ggactcaggg
     8281 tagagcacac ttcactgaaa ggcaatcaat tgggcaacga gtttcagcct ggctcgaagt
     8341 tttcgcgcaa ctatacacag cctttcccca ctttgtatgc caaccttcgc ctcgattcgt
     8401 tagacaaaaa caccctgagt ttttcctatg gccgccgcat tgatcgcccg ttttttaaag
     8461 acctcaaccc ttttatcagc cccttagaca agtttacctt ctacaccggc aaccccaacc
     8521 tgctgcccac ttttgcgcac aatctttctc tgacgcattc ttttcaggga aaagtcaaca
     8581 ctacgctatc ctattccaag actctggacg gcatcaatga aacccttgaa atcgtgaatg
     8641 aaatttacta tagccgcccc ggaaatatag ccaattccca atccatcggc ctcagtctgg
     8701 aggctaccct gcctgttgcc aaatggtgga cgctcatgtt gtatgccgcc gccgaccggg
     8761 ttacatataa aagcgatctt tacacccaac gccttgatgc cacagggtac aacggctata
     8821 tttccatgac caacagcctg caattaggca agggatggtc gggcgaagtg cgcgccgact
     8881 atcaaaccaa tcaggtgtat gctcaattgc ttannnnnnn nnntcctcaa cttcggcacc
     8941 ctcaatctgg ctgcggcaaa aaaaatatgg aaagacgcag ccaccatcaa actttcagca
     9001 caggacctgc tctacacccg ccggggcagc ggcatcatca acaaccttga actcacagag
     9061 gccaactgga atagccggtt cgattcgcgc gtcctcaacc tcagcttttc ttatcgtttc
     9121 ggaaaggtgg ccaaccagcg ccagcgacac aacagttccg gatcggaaag tgaacaacaa
     9181 cgcgtaagat cataatcggc aaaatctgta ttttcgccgc ccgatgcgcc attgctctca
     9241 ggcagcgatg tgcaccggcc aaacgatcat accaaacatc aaactcatgg aaaccgtgga
     9301 aaaatacgtt gacatcatta ccgaaatgct gatcaaatac acacccaaag tgctgtttgc
     9361 gctggtgctg ctctggatcg gatttcgggt catcaatcgc ttggtagata ccggtctcaa
     9421 agccatggag cgcagcggca tcagtcagga tgtgcggccc tttctggcat cgctcatggg
     9481 gctgttgctc aaagccctgt tggtattcag cgcggcgggc attgtaggca ttgaaaccac
     9541 ttcttttgta gcggcgctgg cagcagcggg cttcgcggtg ggcttggcct tgcagggcag
     9601 cctgggcaat tttgccgccg gtatcatcat cctcattttt aaaccctaca aagccggcga
     9661 tttggtttct atcggggaca ataccggcca cgtcgccgaa gtgcagattt tcaataccgt
     9721 gctggctact ttggacaaca aaaccgttat cgtacccaat tcggaagcca tcaacggggc
     9781 cattaccaat ctgagtaccc gccaaattct gcgcgtggat ttggaagtac ccatgcctta
     9841 cgcggaagat ttcgaccgca ttgaggccat cctgctggag gcactccgac aaacgccgaa
     9901 agtgctgcaa gacccggcgc cttttgtggg tattcaaaag tttgacagcc acagcatcat
     9961 cctggatgtg agaccttatg ccgacgcgga ggactactgg gaggtgtatt atnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnntagcaa accatgcagg tgcagacttt caccgaccaa atgggccggc aggtggaaat
    10201 acccttcccg ccgcagcgca tcgtgtcgtt ggtaccctcg caaacggaac tgttgttcga
    10261 tctcggactg ggtgagcgtg cggtaggtat caccaagttt tgcgtgcatc cgcaagtctg
    10321 gtttcgcagc aaaaccagag tgggcggcac caaacaactg cgcatggaaa ccatcgccgc
    10381 tctgcgcccc gatctcatca tcggtaacaa agaggaaaat gaacgcctgc aaatcgagca
    10441 gcttgccgcc cattaccccg tttggatgag cgatgtgcgc gacctcagct ccgcttnnnn
    10501 nnnnnnnnnn nnnnnnngtt gggtcgcatg accggcaaag ccacagaagc catttccatt
    10561 gccgaaaaca ttcaatcggg attcgatgac ttagctgtgg ctgttgccca acagccccgg
    10621 cttcgggcgg cctatttcat ctggcgcgaa ccctggatgg cggccggcag cgataccttt
    10681 attgatgaaa tgctgcgttg ggctggtttt gaaaacgcct ttgcgcacct gcctcgctac
    10741 cccgaactga gtctggaaca attggccgca gccgaaccgg attgcgtcct gctctcgtcg
    10801 gagccatacc cttttagaga aaaacaccta ctggaaatca gtcgcttgtg ttcaaaagca
    10861 gccattcgct tggtggacgg cgagctgttt tcgtggtacg gcagccgatt ggcacagagc
    10921 ccggcgtact tggcggcgct gcgggaagcg cgctgtcgga agtagtggac taaattacct
    10981 cactgaacga aacttcttca taaactcatc tgaagaatta ttctttttca aatccttcat
    11041 caggcgttca acgatggcgc gcatgtcggg atgatcggga tgcaggcagt cgttgcccgt
    11101 ttcgtcaaat accctgaatg ccgtaaacat caggtcgctt tcttctccat acgaaagcac
    11161 gaggtagcgt ttttcgtcac ccaacaatgt aacgtttttc caaacgaaca ccggcctgat
    11221 accggaggcg ggcacggtca gcttttccga ccaatcgccg aagcggatga acaaatactc
    11281 ggaggactcg taagagaaac cgaggcagtg gaactggagt tcgtaagatt ttcgaacagg
    11341 gtagttcttg acaggatatg cttctttagg atttgccagc agcgcatacc cgtagctgtc
    11401 gttggggaaa gcagtgcatt tttttctgct tttaagtcca tacttcagcg agggtatttc
    11461 ggcctgagcc gacaaatccg gggagcaaag caggcaggcc atagccgcca aaaaaataac
    11521 tgatggacgc atgagggttg aatttagtga ggatacaaag gtaattttta taactttatc
    11581 cctcaataaa aaatcatccg ccatgcgcac caaccttttt cccatcaccc ttttcgctct
    11641 ggccctggcc gctttttctt gtagaaccga aaccgaaaaa acagcccagg ccagccccga
    11701 ccgtcgctct cctgatgaaa tttacggcga gcttttccat gccgtgcaaa cctcctccgt
    11761 cttccccgac ggcaaaacct tcgtggattg cagcccgaaa tttcctgccg ataccatcat
    11821 ggcgcgctac acagccgccc gcaatgccgc cgatttcgac ctcaaagcct ttgtagccga
    11881 gcactttgat ataccgcagt ccggcgcttc cgactttgtg agcaacccca agcactcccc
    11941 tgccgaacac atcaacgcgc tgtgggaagt acttacccgc cgccccgatc aggcgcaggc
    12001 cggcacgctc attccactgc cgcatccgtt catcgtgccc ggcggccgtt tccgagaagt
    12061 gtattattgg gacagctatt tcaccttgct gggcctgcag gacgccggtc aaaaagaaat
    12121 ggtgcgccat atcgccgaca actttgccta cctcattgac accatcggct tcgttcccaa
    12181 cggcaaccgc acctactatc tggggcgctc gcagcctccg tttttctcta tgatcgtgcg
    12241 catcgtagaa gaaatggaag ggcaggaggc cgccctgaag tacctgccgc aattggtacg
    12301 cgagtatgat ttctggatgg aaggccaaaa acgcctgtcg gaacaaaacc cgacgcatcg
    12361 ccgcgtggtg cgccttgccg acggcatcgt gctcaatcgc cattgggacg atcagccccg
    12421 cccgcgcccc gaaagctacc gcgaagacct ggaaaccgcc caaaacaacg cccgcgaatc
    12481 gcaggccatc tacaccgacc tccgcgccgg cgccgaatcg ggctgggact tctccagccg
    12541 ctggctcgaa gacccaatgg acctggccac catccgcacc acggacatca ttccggttga
    12601 cctcaatgcc ttgctcttcc atttagaaag catcattgcc gagtatgccc gcttagccgg
    12661 caaaaccgat caggcagccc gttttgagca gttggccgcc gagcgcaagg cgcacatcgt
    12721 tcgttattgt tgggatgaaa cgagcggatt tttctatgat tacgattttg aaaacaaacg
    12781 ccacaccggc gtcccctcgc tggcgggcat gtatccgctg tttttcaaag tggccgacca
    12841 aaaacaggcc gaggcttgcg ctaaagtcat tcaacgcgac ttcctgaaac ccggcggcgt
    12901 cgtcaccacg ctcacacaaa ccggacagca atgggatgct cccaacggct gggcgccact
    12961 gcaatggatc accattcagg gcttgcgcca atacggacaa acggcattag ccgacgacat
    13021 ccgcaaccgc tgggtagccc tcaacgagaa ggtgtatcgc aacacaggaa aaatggtgga
    13081 aaaatacaac gtgatggaca tgacattaga cgccggcgga ggagagtatc ccgtgcagga
    13141 tggcttcggc tggaccaacg gcgtgctgct ccgactgctg acggaggaga agaagtagga
    13201 ggtttttatc cttgcctcat caatcgcgca aatcctctac gcgatagccg ggaaacttgc
    13261 ttttggaaaa gctgaaaaaa tcggcggcaa aggttttgtt gggagttaag gcgcccattt
    13321 tcacggtgta gcgggaggcg tctttgccga aggctttcat gctcactacg gtgacagccg
    13381 ctttgtccac cgtgaggcga aacttggcgt attccgaacc tttgtccaag ggtttgaact
    13441 cgatttcctg cactaccttg cctgccacgg tggcttcatt gaccaacgaa taaacaaatt
    13501 tgcctttgtc gtagaagctg aacagggctt ccggcgagag annnnnnnnn nnnnnnnnnn
    13561 nnnatgttct cgtccgttga aggatcgggc aggttattga tttgtacttc tttgttgttg
    13621 tgcagcacca accacagcgc tgtgccgtcg cagatcacag attgagaagc cagatcaacg
    13681 cggtattttt tgccctgctt ggctattttg ccctgctgga cttccttttt ttgatcgggc
    13741 agttctattt ccagggtaaa agcaagctcc agggattgat aaccctgata cttctttttc
    13801 agtttatcga gtacggcttt ggcttgcgga tcggattcct gcgctttggt aaattggccg
    13861 gtttgctttt gagcatgcac agacagtaca cagaaaagcg caataacgag catccagatt
    13921 ttcttagtca tggcaatttc tttcagttgg taaacgaaag gagaaacgcc ggctgagccg
    13981 acattattga caataaaatg cgaaggagag gttaatgttt atttaatgac accgaaacgg
    14041 aatcgggtag gcagcggcct gtggggaaaa ctttttcacg cggcgatccg gggcgagctt
    14101 gccccggtcg gccgacgtga tgatatcgtt caggttcatg cgggctgcaa atgtcccatg
    14161 ttgtaaagct gctgaccatg atggtacaag gcgctgcgga aagtcggcat ttcgtgatag
    14221 ggcaggccga attctttcgc tgtttgcttg acgatgggag acaacttttt atagtggatg
    14281 tggcaaatat tgggaaacaa gtggtgctca atctgatgat tcaaaccgcc cacgaaccac
    14341 gaaaggatgc gattgcgggg agcaaaattg caggtggtgt gcagttgatg gatcgcccaa
    14401 ctgctctcca tattgccgtc ttcgggcgcc aacggaaaat cagcttcggg taccacatgc
    14461 gccggctgga aaaccaacgc taaaatgagg ccggagatga ggtgcatggc tatgaagccc
    14521 agtacagtaa cccaccaggc taccggagaa accagcaaag gaatgaccaa tgcgtacaca
    14581 taataaacgg atttggaaac gatcagctcg gccagcagcc cgccataagt gcggccttgc
    14641 gcttcgatga taccttcttc tttccaacga ttgatttgtt taaactcctt tatggtaatc
    14701 cacatgatgg tcatcagggc atacaaaggc catgcataga tgtgctggta gcggtggtgc
    14761 ttcatccagg ggtggtgcgg cgagaagcgg agcgctttgc gcggctcgat gtcttcatca
    14821 tagccgtcca cattggtaaa agagtggtgc aacacattgt gctgtatttt ccagttcatc
    14881 atgttgcctc cgaggaagtt gagcgtataa cccagcatgt tgttgatgga ctgtttgcgg
    14941 gagtaggctc catgattggc atcgtgcatg acgctcaggc cgatgcccga aaccccaagg
    15001 ccggctaagc accacatagc gaataccatc cagggggatg tgacggtggc ggtgataatc
    15061 aacacaacgg gtacgaagta gaggagcagc atgaagatgg ttttgaaaat catggctgcg
    15121 ttggcatggc gcgatatttt gttttccaca aaataatcat tcaccctgcg gttcaaagtg
    15181 gtaaaaaagt ctcctgtctc cggtttggag aatttgatga tgttggcgcg cataatggta
    15241 aaacagattt gatgaaagaa ctcaaaaaca caaaagaaag gccgaagata tgcacgaata
    15301 gaaacagggt gtatgtattt gtgccttatt cgccctcttt tttaggtttt atgacattct
    15361 gcttaccatc ttcgccatac acttcgcgcg attttttatt atatgcaagc gccgcctctt
    15421 caggggtaga gaacatgccg aggtgaacag atttgcggtt gatggagatg acggcccggt
    15481 agcggttgtt ttctttatac accccggtgt agcccatgtg gccgctggtt ttgcgtttgc
    15541 ggctggccac cgaacgcgac cggtaaatga ggttttctac gcggcagtcc aacttattgt
    15601 tgttgcgggc gcccaccaaa ttgcactcct gcgttttggt atgcgcgaga aacttttcgg
    15661 caaccaactt gtgcagatag atggtctctg ttttgaaaga gccgtctgcc tttttccagg
    15721 ttttctgaaa tacggcgcac ccgctggagt gcatcctgag attgttgata aagtccaact
    15781 tggacaaata cgaatcgctg atgagccatt cgtacacgcg gtcatccaga ataacttctt
    15841 ctgctgcgtt tttcaatttt actttgtaga gcacgacatc ccgattttaa tgaagccaac
    15901 acattaggcc gggtttgtta aaatgggatt catcggtaat atatgcaggg attatgaaag
    15961 gaaggccaaa cccaagcccg aaaatcgtcg cgaaggtaag agataattag ggctttaaca
    16021 cactttcttc attttttctg tgttattttt acctcctgag ccaaaacttt tctcttaaca
    16081 atggattttc acacaagatg actctgcacg aaacatacat gcaacgctgt tttgatctgg
    16141 ccgcactcgg cgaagggcat accgccccca accccatggt gggcgctgtt ttggtgtatg
    16201 aggggcgcat catcggcgaa gggtttcatg ccgaatatgg aggcccgcac gcagaagtaa
    16261 atgcggtggc ctccgtgccg gcctcgctgc ggcatctcat accggcctcc accttgtacg
    16321 tctcgctcga accctgctgc attaccggca aaaccggcgc ttgtaccagc ctcattttga
    16381 aagaaggcat caaaaaggta gtggtttcct gcctagacat gactccgggg gtggccgggc
    16441 agggcgtcgc tttgctgaaa gccgccgggg tggaagtaga attgggcctg ctggaaccgc
    16501 aaggccattt tttgagccgg gtgcgcaatc attttgtcac gcggnnnnnn nnnnccctta
    16561 cgtcatggtc aaatacgccg ctacgcccgc cggctgtttc gcgccccaac ccgctgcccg
    16621 cgcctggatc acgcatcctt atacacagcg gttgacacac cgcctgcgca gcgcgaccgc
    16681 tgccatcctc gtcggcacac agacagccct gctggatgat cccggcttgg acaaccgcct
    16741 attttacggg ccttctcctc tgcgtgtggt actggatcgc cggctgcgac tgcccggcca
    16801 tctcaaaatt ttcgacggct ccctgccaac actgattgta catgaatcgg aagccggagc
    16861 caagactcat cgggccgcgt atttgcagct cccttttgac gccgacttgt tgaacaacct
    16921 gctcgctgcg ttgtacgaac gaaaaatcag ttcgcttctg gtagaggggg gggcgcagct
    16981 cattcagtct tttttcgatg ccggactttg gcaggaagcc attgtttata caggcagccg
    17041 ggagtttgaa cacggcctgc cggccccgcg cgtgccgggc gtatgtgtac aaacggagca
    17101 aatcggcacc gacagcctgc gccgatttgt accgcatagc tgttaaactt cctttaaagt
    17161 tggcctgtat taccataaac cggtttttaa ctttgttccg gtagtggtaa ttttaccgcc
    17221 tcaatgttga acttactatg agcagatact ccctgatttt ttcattggcc gtcggcgcct
    17281 tgttgttttc gatgaccgaa gcatcggccc aaaacgccgc gcctgctgca tccgcgcccg
    17341 cagttcagtg gttttcgtgg aaggaaggca ttacaaaagc gactagagaa aacaaaaaaa
    17401 ttctcctcta cgtctatacc ccctcttgtg tgtggtgccg caaaatggaa cgcgaaacct
    17461 ttgccgatga aacggtggcg cagttggtaa aagccgattt tgtagccatc aagctgaatg
    17521 cttccgacaa tgcagaactg gaattcaagg gcaaaaccta tcgtttcgtc aattccgaaa
    17581 caggcggctg caacggcctt gccgccgaac tgttgggcgg aagaatgagc tttccggcgg
    17641 tagctttttt agacgaaacc cagcaagcgc ttcaatcgct ggtcggcttc aaatcggccg
    17701 aagacttcgg ggccattgcc acttattacg gcaaaaacta ctaccagacg atgccctggt
    17761 cttcttttca gaaaaaatac gctgccggcg gaggcaggta aacctgcatc aggactccac
    17821 ataatacatg gctaccggcg gaagattttt gaccggcagt acgcctctga aagtgcagct
    17881 aaactcgctt ttgaccaatt ggaacgtgct ttccgaaaca ttcacccgcc cgccttcccc
    17941 ggcactttct actctggagg ctatattgac ggtgtcgccc caaatgtcgt aggcaaactt
    18001 gcgatagccc actacccctg ctaccaacgg ccccgtatga atgccgatgc gcgcttcgag
    18061 gggcgcttct ccacgttcgg cgcgctgtgc attccacatt ttgaggaaga cctgcatttc
    18121 gagggcggcg gctaccatgc gcagggcatg atcggtatca gattggggca ggccgccggc
    18181 gcacatgtat gaatcgccga tggttttgat tttttcgagg ccgtgctttt ccgtgatttt
    18241 gtcaaattgg ccataacaat agttcaaatc ggctaccaat tgttcggcag aaactgcttt
    18301 ggcaatgcgg ctgaacccca caaaatctgt aaacagcacc gtggcttgtt cgtattgccg
    18361 ggcttgcgcc gcgccgcttt gcttcaattc ggaggcaatg gccgccggca ggatgttgag
    18421 cagcagttct tccgagcgtt tctgggccgc cgtaatctgt tcgtttttaa tcgtcagcaa
    18481 agtattatac ctgcttactc ctctgtaccg cgcatatacg cctccggcaa cgaccagaat
    18541 caacaaagcc agcgccgaca agaggttccg gcggttgcgc tcggtagcca gttcggcagc
    18601 ctgttcttgt gtttgtgcct gactgagggc cagagccatc gaatcgcgga ggccgcgcat
    18661 ggaaagcgag tctaatacgt tgcgttggcg ggagtacaac aactctaagc gggcctgctc
    18721 gtcgctcatg gccgctattt ccttttcctt gtctctcaaa attttttcca tgtcggcctg
    18781 tcggcgcgcc gattcggggg tcggctgccc cgatgacaac ctggaggaag ccgcagcgcc
    18841 gggagaagct accggaggag gctcggcagc cagtggctcg gatggggccg ctgccggcga
    18901 tttggccccg gccgcgactt ttttttgtca aattttgccc tgcacggtca gcgatctgcg
    18961 cacccagtct ttgtggttgc ccgcttcggc caaggactgg gcctgttggg cgtgttccag
    19021 cgctttgtcg tagttgtctt tgtccaaata cagattggcc agtgtgattt ttgtctgaat
    19081 cagctccgta cccgtttggg tttccagctt gcgctccaat tccgtgatgc gtttggactg
    19141 ccccatgcag gcgatggagg ctgacatcag gatggctatc aacagccggt atttgagtgg
    19201 caacatgggt tggtgtgtgt tttcaagtga agtatgtgat acgaggccaa agatacttat
    19261 ttcgaatctt taaagtatct caatgccccc gcatctaaat gataaaatgc cagcacagat
    19321 tggtcggagg agcgctgcat ggcttgtttc aaccattggg cgcattgttt tacatcttca
    19381 ggatcaggca gttctaagcg caggcgatct ccggcataca ggtaatgccc ttccatccag
    19441 gtgcttttga ccaccttgta ccggtgaggc gccgtgcaga caaacctcgt ggtatcggcc
    19501 aattgttcgg gttgcagatc gtgaatcagg cgaatcatct ggtcgttgcg aaacaagacg
    19561 ccccaactga aaaccggcag ggcgccatcc aagggcaagg gatacggggg ggcatttttg
    19621 aaatacaaag cagcctcctc tgcgcggaga atggagtttt cttcatccca gttttccaaa
    19681 tcaccggtgt tgtagcacat caacatgccc cggtcggcgg gcggcactcc ggtcgtttcc
    19741 gggctgctga actgatgcag gcgcacggtg acagatagtg ctatgttttt gtcattcaat
    19801 attttacgca aagattccaa caggacaaag tagcgctccc gcgtgccggc ggtccagtcg
    19861 caatccgcct gaaattctga aaatcgcaag ccgggatact gtgcggccaa ggtttctgtt
    19921 ttttgccaaa tgcgttgcgc cagttcggcc gtgccttctg cgggcagttt ttccatgcag
    19981 cggttggtga taaaaatgca gggcacgatt tctaagccct ccagccgcgc cgtgtctatc
    20041 ttcagttcgg ccagcggcgc gggtacgtcg taagcctcgt cccaatccac gtcgaaaaag
    20101 cgaacgtaga gcctgcgcgc cttcatcccg gagagcagtt cgcgttcctc gctgctgagc
    20161 gagagctggg ttttccaatg ataaaatgcg ggcaagggtt cgtgcgcatc gcggtggcag
    20221 gccggcaacg cgaaaatggt taaccctaaa gcaagtatat aaaaatgttt cattgtcaaa
    20281 aatcccttta cctttgcccg tcgaaagacg ttccaagttc ctaaccataa ggtctttgcg
    20341 gttgttttgc aacagccatc ggacctgaat ttcataccaa ataaagacat cttatatcac
    20401 ttgacatagc ctgttgtcga agcgggttca ctaaaatgtt gtttatgaat gagtttctgc
    20461 cggaattaac ggctgctttt aatgaaaaag gggagacgat cagccccaaa ttgcccgaat
    20521 gggtgaagaa tttcctcatt gatatagacg gtaccatctg tgacgatgtg cccaatgagg
    20581 aacccgaacg catgggtact tgcctgcctt acccggacgc gctcaagtac gtcaacaagt
    20641 ggtacgatga gggccacatc atcacgttct ttacctcgcg caccgaagaa caccgcgaga
    20701 ttaccgaaga gtggctcgac cggcatggct tcaaatatca cggcgttttg tacggcaaac
    20761 cccgcggcgg caactaccat tggattgaca accacatcgt aaaagctacc cctttcaaag
    20821 gtaaatttac agacttagtg gtgcaaactg ctgaaatcga ggtttttgcg ggatagcagg
    20881 ggtgagtagg tgaggggtta ttccttcttt tttacgccgg ctttggcctc tgtttcccag
    20941 ttgccgcgca attgctcaat cggaaagagc atgatttctt ctggttggat tttggtatnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nntcagcaaa gacacgatgt aatctgtctc cggctccatg
    21181 tcttccacga tgatgtgcag cgtggcgaag ttttttcttt gctctatgcg ccatttcagc
    21241 agcagcgtat ccgcattgct caggccgtac atatcggtga aagcgccggg cagggcctgc
    21301 atttcataaa tgagcgcttc tttccagggc aaactcacat tccacacgcg gggctgcacc
    21361 gaatcaggct tccatgttgg aaatacgggg ttgcgcaggg tgtcttccca ccaggaaata
    21421 aggctcgtat cggcgcgcac aaccgggtgg ttgaacgtga gcgccgcggg cttgtcgggg
    21481 tgcaacaagg cagtggcgcc gggcgtgggc gagaccatgc tcagcttggc tttgctcaaa
    21541 aaagtcggtt tcagcgagcc gtttttaggc gtgatggtgt ctgttgtaat cgtatcggcg
    21601 cgcacgaaga gtttccaact attggtatcg gggcggtcgt accatatttt gatcgaatcg
    21661 ccgagatatt ccgttttcca tatttgggcg gtgtctgatg cgctgaaatt ggtggaaagc
    21721 ggcgtttggc tgtaggtcag ttttacgact ccgtaagtag ctgtttcctg ctccagcaat
    21781 ttgatgggtc gggcttcctc aaacagccgg atgcggagat cgcgggcaga gctgtcggta
    21841 gtaataatcc atgcgtcagg gaatcctatg agttctttgg actgattgaa gcggtagttc
    21901 agatcggcgt cgagcagcgc aaaccccttg agcgtatcgg cgcgcacatt gggaatggtg
    21961 aaatggccgt ttttgtcggt gcggccaaaa taaaaaggca attcggtgcg caccaccgag
    22021 tcgttgaggt tgtcgtacag catgaacagc gccttgtcca ccggctcccc ggtgaggtcc
    22081 gaaacgatct tgccggaaac ggtgagcgag tcaatataag ctccggtgga aaaaacaaag
    22141 cgcaggtttt cggcggggtt gttttcggtg aggtctttga cggcgttgcc gaaattgatg
    22201 gtataagtgg cgtcggggcg caattgctct ttttcgttca attcaaaaac aaccgttcgg
    22261 cctttgagcg aaaccgcggg cttgtattgc agcggcggcg aaatgaccac ctgattgaac
    22321 acatcggtga gggtgaccca ttcgtcaaaa accaattcta tgcggcgcgg cgaggagttg
    22381 gcctggaaat tgggcgttga ttcctcttcc aataccttag gcggcgtttc gtctttgggg
    22441 ccgccttcca agggcgatat ggacgcgcag ccggccataa aatacaccat tgccacaggc
    22501 aacgcgaggc gggcaaacat caaaaccgaa cgaagcaagg caggcatgac gtttatctta
    22561 gtatggtgac gctgccggtt ttagattgct cttcgccatt gcagcggtag cgaatccggt
    22621 agagcatgac gccgggattg acgggcgttc cgttgaagct gccgtcccat ttttcaaaag
    22681 cattttcggt gtaaaacacg cggcctcccc agcggtcgaa aatttcaaac gaaatgagat
    22741 cggtggcggc aaagggattg ctgaggccga agttgtcgtt cagtccgtcg ttgttgggcg
    22801 aaaaagccga gggtaaaaac aggctgttgc agtccaattc attgcggtcc accacggtga
    22861 tgcggatgct gtcggtagcg atgcaggacg aactgccgtc gcccatgctg agcgcataat
    22921 tgaacacgcc tgccagcgga gtcgtgatgt cggtttcgcc ggctgtgggg ttggctacgc
    22981 cgctttgggg cgaccacgaa aactgtgtgg cgcaagtttg ggccaattcc atactcaccg
    23041 tttcgcccag ataaatgagc gtatcgcggg agaggatgcg gtcggggccg aagtacaaat
    23101 cgaggatatc gcgttcatcc aagcccctgt tatacaccct gaattcatct atcagcccga
    23161 cgaaaggcgc ttcgttggcg ccgaggcaat cgctgttgcc gatgatgaga tcgccgtcat
    23221 tagtgaggtc aatgcggctg gcctgcaatg aaacctgctg ccgctcgccg ttgagataaa
    23281 aactcacgcg gcccccgatg cgcgagatcg ctgaagaaaa gcaggccgtg agcctttccg
    23341 gctggctcgc cctgccgctg tggttcgccc tgctgatctg gaccgtc
//
LOCUS       DAORLF010000018        23352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1206_length_23352_cov_2.000687, whole genome shotgun sequence.
ACCESSION   DAORLF010000018 DAORLF010000000
VERSION     DAORLF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_23352_cov_2.000687"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK198_02970"
     CDS             complement(<1..282)
                     /locus_tag="PK198_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37727.1"
                     /translation="MSNTTSIPNPSRHDVVTFDILSEGSVIDPAYEVMSISIVKEINR
                     IPSAKIVIRDGDASQRTFHVSEQDDFVPGKKIAIKIGLDYFGSKMRPMVV"
     gene            complement(286..>655)
                     /locus_tag="PK198_02975"
     CDS             complement(286..>655)
                     /locus_tag="PK198_02975"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF37728.1"
                     /translation="HRPHFLKIVWGEHLFQGILSNLDINYTLFHPNGKPLRAKISATF
                     LDYVAQEQREQEARRQSPDLTRIRRIKAGDRLDKMVGEFYNDPKFTQQVARANNLTTF
                     RLIKPGIELSFPPLDKTEDA"
     assembly_gap    656..669
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<670..1025)
                     /locus_tag="PK198_02980"
     CDS             complement(<670..1025)
                     /locus_tag="PK198_02980"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37729.1"
                     /translation="MEKLKIKAFKNRDFTGEAGTISLPVNPESYSKNYKVEYDNRRPH
                     GSQGNSGRFNSTAPEQLKLDFYFDGTDTIQGYDYDGLAKNRTESDATVTNRTVPRDDL
                     VSQQIERFLNTVYDMAG"
     gene            complement(1225..1683)
                     /locus_tag="PK198_02985"
     CDS             complement(1225..1683)
                     /locus_tag="PK198_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRF37730.1"
                     /translation="MAEYRWQHVNFHFVVSFNGLDSKHIDAGFQSVTGLDVQIESETI
                     KEGGENGFEHSVPVRRKYSALTLKRGILTGREKSVIYDWCKDAFEKGIVRPANLDVLL
                     LDENHKPLMKWKVEHAWPKSWKTGELNAERGEVLVETLELSYNRFEFSNV"
     assembly_gap    1854..1900
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1901..2226)
                     /locus_tag="PK198_02990"
     CDS             complement(<1901..2226)
                     /locus_tag="PK198_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRF37731.1"
                     /translation="MAREGGYPLPKFHYQVNYFGFEFGFTEVSGLDFETEVIEYREGN
                     APLYHKTKQPGLTKYSNITLKRGMFKGDNKEFDHWKKTFYFQEGKAEFRGDCTISMLD
                     EEHNPV"
     assembly_gap    2377..2386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2387..3400)
                     /locus_tag="PK198_02995"
     CDS             complement(<2387..3400)
                     /locus_tag="PK198_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37732.1"
                     /translation="MARSKIRERDTVHIDCEWQDNANCPPGEDCIEKWINEKAARKKD
                     LDVAIARLENAKKNLAYIEAWERRLKLYFDGIEKTADLADKVCREAKTTIRQFEKICQ
                     NADKSVTAIQILYCEVEKVFRNENNVDVETLTSMMEGIMKCLRCITDPGLIRDRGIVK
                     ALEAFDAKLKELAALQLDAMKKIIEALKCANLLYHALCDIPCRDENDSPIPVTDCQDY
                     GCDSLMSELRELQACFGTCNEPEDCNGGAGTDDSGPGANAGNTQEEYPWADCCPKDDK
                     VWECQLGCDQQPHTAALAPRPIFPLTSSDYYTQTEAQWTCAKVEKEKKKCELDKAKEE
                     KDRL"
     assembly_gap    3604..3640
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3641..4416)
                     /locus_tag="PK198_03000"
     CDS             complement(<3641..4416)
                     /locus_tag="PK198_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37733.1"
                     /translation="MGTTIQNGGTKTGDPADCLKKKDILHDYKKHLQNNKTEKNGIAE
                     AAKETTKGYLQDRNGKVCFVLKAEEAVRFYRNIDLYVGIQSTCVAEAAQKNVDEQIKK
                     SDDLAKALQEVSKSLKTLKDKAAELKAKACELDTQLKDDCNREQLRILNCNFEQTCGY
                     PKSGTTEAFTALAEQLIDISKAVCSQASKTFNDSAQIAGIQTFSNVKSLKDMSKMIGD
                     AVKSFKTDVDTNIKDRSADETKDREELAKAVKELTVKKLE"
     gene            complement(4465..5886)
                     /locus_tag="PK198_03005"
     CDS             complement(4465..5886)
                     /locus_tag="PK198_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HRF37734.1"
                     /translation="MNYKTPGVYIEEISKLPPSIAQVETAIPAFIGYTERAEDARGLP
                     LLNEPKRITSLLEYETFFGGAQAETSITVTISESPGRPIQVNAAIGADRSKYLMYYSM
                     QLYFANGGGPCWIVSVGDYSTGIVGAAELRAGLNTTEKIDEITLVVFPDAQNLTTSSD
                     YYTLYGEAMDLCEKMQDRFTVMDVWNDPTLPVSDWRTNIETLRNETPGEINTLKYGAV
                     YFPNVETILDYYYGGEGPGDGNVQIVHTGGDGSYNGTLAELRSKNNALYFTARSKVRD
                     LPMELPPSPGMVGIYANVDTQRGVWKAPANVNMDFVIRPVIKITDEEQRDLNVDTNAG
                     KSVNVIRSFTGRGPSIVWGSRTLAGNDNEWRYIPVRRYFNMVEESVKKATIQFVFEPN
                     DANTWTRVKSMIENFLSLQWRGGALMGASTEEAYFVKVGLNETMTELDIWEGRMIVEI
                     GMAVVRPAEFIILKFSHKMLSES"
     gene            complement(5901..7265)
                     /locus_tag="PK198_03010"
     CDS             complement(5901..7265)
                     /locus_tag="PK198_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37735.1"
                     /translation="MADIIFRRLFEIRILHGYYLDYWFPDSSNNPGVFHEFRDEPQSS
                     RLAKQASVLEYKYDLRKIVNIEPTKETQQWLSRNKIILRQTPLGIFAGIQARREGTGA
                     SARFYPIGELEQGNCRFVLTMRDPQWLNAANHALMPTIPGSYYFTNLRATEDGKIYPS
                     LSAQPPEFESGRTWEMGGLIREGNQIKAAQRTTETIVDFQAIGDTAAGTWHSYAHAGE
                     RVALPKTFRYRFDPSIPIVSNAEFIIRDLYNNEIKRIAISNPSSSSVSLDFRYLPVSP
                     STPENDRIRARPIHDGVYVLDAFINGTLFEQRQIVLSDDLRGSDKNVWGLVDISTENT
                     APDFRLFNADGSLNAGLTTPAPAMRWRGPVFEIRLMSRFTFWRYHIERLSSSIPGDPD
                     FSYNWPEQQFTTAHPRRLSQIRVPLSINLPSGAHLLPNPSSASIRYDEAQKQYYSDIF
                     LLTF"
     gene            complement(7308..7925)
                     /locus_tag="PK198_03015"
     CDS             complement(7308..7925)
                     /locus_tag="PK198_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HRF37736.1"
                     /translation="MIHQALNLLHHELEEYLQNRLGPNTENYAELSNVGQIENENATG
                     LDGKVVVSLVNIEEESTLKNNPHFKKTINGGVRYENPPVYLNLYLLFTAFFPPTPDGY
                     RESLGRLSSVIEFFQGKHVFNLRNAPGFLINEPDLADIQLILNLYTMTFEQINHLWGS
                     LGGKQIPFVMYKARLVRMLDRRQTGSGMLIEEIENNIGSTQAPGR"
     gene            complement(7922..8464)
                     /locus_tag="PK198_03020"
     CDS             complement(7922..8464)
                     /locus_tag="PK198_03020"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRF37737.1"
                     /translation="MQHQPSSAAEISWYALLVKPRTEKKVGQRLGELGFEACVPTQMQ
                     LRQWSDRRKKVEVVLFNNYVFVATDNKRRNEVFQAGHVLKYVQFGGRIATLSEKEVAM
                     IKRLARLEAPVEITYERFRVGETVEVLTGPLAGYCGTVMSVSGHARLQLALPSLQCFA
                     RVEVKGEEVKRVVNDMSYTL"
     gene            complement(8664..>9067)
                     /locus_tag="PK198_03025"
     CDS             complement(8664..>9067)
                     /locus_tag="PK198_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37738.1"
                     /translation="THIIMYTVYEDDEKLFDSLCAGASGYLLKKTPPDQLTKAISEVM
                     EGGSPMSPGIAQKVVQYFHKLPKKSDHHLCERELNILRQLVDGLPIKIIAGEVHLSED
                     GVKKNLANIYKKLHVSCGKEAVAKAVREGIV"
     assembly_gap    9068..9263
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9372..10121)
                     /locus_tag="PK198_03030"
     CDS             complement(9372..10121)
                     /locus_tag="PK198_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF37739.1"
                     /translation="MLIFILLRQRKAMRKLRSSIACDLHDELGASLSNISMLAGLVSM
                     ELLNGDKELAQKYLDRISEEADQVHNSISDTVAVLGSSSRQLGHLAALINRHGHELLE
                     PKGIRFSLSVAPPLKAIRIPAIQRRHLYLIIKETLHNAMRHSDAGEVNVRFSLDGRRL
                     YCSIQDNGKGFNTEKTYFGAGLGSMKMRMIGMKGQLNIQSSPGNGCHIQFSMPVLPWW
                     RPAWGWSHPQEKEKLAPLAAKTVLQHLPAEV"
     assembly_gap    10601..10679
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10680..>12642)
                     /locus_tag="PK198_03035"
     CDS             complement(<10680..>12642)
                     /locus_tag="PK198_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF37740.1"
                     /translation="EGTLAFSTEIKLNLSPLPPPQDALVCAHFDTLDAALRAAQIAMR
                     RAPYACELMDKFILDCTKANRSQQKNRFFLQGDPAAILMVEFRGQTFEEAEAEANAMI
                     ADLRAAGLGYAFPIVSGAQSQQVFALRAAGFGVLSNVTTDAKPLEFVEDAAVDIADLP
                     EYIADFADMMASFGQQCVYYAHAGAGELHPRPSLDLKTSEGVRQLREIAEASARLIKK
                     YNGSLSGEHGDGRVRAEFIPLIIGEDNYALLRRIKQQWDPAGIFNPGKIVDALPMDTS
                     LRYLQDAPPPKIDTVFDFSATKGILGLAEKCSGSGDCRKLPHVTGGTMCPSYMATRNE
                     RDTTRARANALREFLTRSENGENRFAHEELYEVMSLCLSCKGCASECPSNVDMATMKA
                     EFLHHYYQAKGVPLRARVFGHIGSINALAATVPGINNFFLKNPLTSGLLKRALHIAPQ
                     RSMPLLQKTTLRKWWNQTGRHLQPSGNEKGKVYLFCDEFTNYNDTHVGIQAVELLTRL
                     GYRVEMPPHPDSGRAHFSKGLLIPARRLARENVRIFRELVGPETPLLGIEPSAILGFR
                     DEYPKLVEPADREAAITLGQHALLIEEFLASEIQKGRISAEAFTEAPARVLLHGHCHQ
                     KALAGVEPSAWALSLPKNYTVEVIPSGCCG"
     assembly_gap    12643..12689
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12690..13412)
                     /locus_tag="PK198_03040"
     CDS             complement(<12690..13412)
                     /locus_tag="PK198_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRF37741.1"
                     /translation="MQDQLSRLAGQLTGELHTGRLLRTLYATDGSIYREIPLAVAYPK
                     NEDDIRLLIAFARREGVSIIPRTAGTSLAGQCVGTGIVADVSRHMTGILELNVAESWV
                     RVQPGVIRDELNAFLKPHGLFFGPNTATASRCMIGGMTGNNSCGSTSIKYGTTRDHLL
                     EIRAVLSDGDTVTFGPLSKEDFEKKSAAPGLEGDLYRQISQELSQPERQTSIREQYPK
                     PGIPRRNTGYAVDVLLQSNIFSP"
     gene            complement(13536..14615)
                     /locus_tag="PK198_03045"
     CDS             complement(13536..14615)
                     /locus_tag="PK198_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRF37742.1"
                     /translation="MHISENIHQAHTLPAAFYREAGTFETVKECIFASTWQWVAESEV
                     IASPQQVWPFALLPGVLDEPLLFTRDAEGRAHCLSNVCTHRGKLVVEQPGSQRILSCG
                     YHGRCFRLDGRFRSMPEFDGVENFPSPADDLSRIPFAEWLGLLFVSLQPAMPFEELIR
                     PITERIGWMALDTLRYSPEHSQDYEVQANWALYCDNYLEGFHIPFVHPALNQAIEFGK
                     YEYELHPYCNLQVGIAKEGEPCFDIPAGAPDHGRRVYAYYYWVFPNLMFNFYPWGLSL
                     NVVEPLSHQRTRIRFRTYYFDNAASERALNQLDLTEMEDEAVVESVQRGIQSRYYQTG
                     RYSPTQEQGLHHFHRLVQQFMNGVG"
     gene            complement(14665..>14864)
                     /locus_tag="PK198_03050"
     CDS             complement(14665..>14864)
                     /locus_tag="PK198_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37743.1"
                     /translation="FVQKAFNIMPRHALHAKSLGFIHPTTGEAMYFDSELPADFQEAL
                     TEWRQYVEQRRAAMEKSGQPL"
     assembly_gap    14865..14895
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14896..15778)
                     /locus_tag="PK198_03055"
     CDS             complement(<14896..15778)
                     /locus_tag="PK198_03055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF37744.1"
                     /translation="MQEEEEELEGSEQQDEEYSDYNEILVDPNQSPIRIDKFLIDRMQ
                     RVSRNRLQNAIRAGSIRVDNQEIKPNFKVKPGQRITVLVPKPPGSGVRIVPQRMPLDI
                     IYEDDDLLVLHKPAGLVVHPGVGHWRGTLVNALAWHFQQNKAPVIGDGQDNRVGLVHR
                     IDKNTSGLMVVAKTEFALSHLAKQFFYHTVQRTYYAMVWGEPKADQGTVRGHVGRDRK
                     DRQMFTVFPEGDHGKWAVTHYKVLERLYFVSLIQCNLETGRTHQIRVHMKHIGHPLFS
                     DERYGGDYIVKGTVFSKY"
     gene            complement(15824..16495)
                     /locus_tag="PK198_03060"
     CDS             complement(15824..16495)
                     /locus_tag="PK198_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRF37745.1"
                     /translation="MKLHRYYQIVAADLASPKEELLNVVTHAMGLLLAIGGTSYLLVK
                     IFVQTLHWSLVTGGLAFGVSLILVYLTSTLYHAANRPRLRRVFNTLDHIAIYFLIAGT
                     YTPFILLFVPTFSGYGVLIGLWSVSLIGLVYKIFATGKHKFISTMLYLAMGWAAVLII
                     KPMIAMVPGDILAWILAGGVCYTLGVVFYLAKKLPYHHAIWHLLVLAGSVCHYFGVLA
                     AVSHS"
     gene            16675..17211
                     /locus_tag="PK198_03065"
     CDS             16675..17211
                     /locus_tag="PK198_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRF37746.1"
                     /translation="MALIKSVRGFTPQNGEDCYFAENATIIGDVVMGDQCSVWFQAVV
                     RGDVNSIRMGNKVNIQDGAVIHGTYLRAATTIGNNVSIGHRAIVHGCTLHDNVLVGMG
                     AIIMDNAVVEENVLIAAGAVVLENSRLESGHIYAGVPAKKVKAVSPEQFKDSIERIAN
                     AYIMYSGWFKDEDAPNAG"
     assembly_gap    17259..17280
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17308..18219)
                     /locus_tag="PK198_03070"
     CDS             complement(17308..18219)
                     /locus_tag="PK198_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF37747.1"
                     /translation="MSTTTKPAPAAAPRISDRWLGFIILGILALTWGSSFILIKKSLT
                     AYSATQVACLRLSITALALFPVVWARRREVEWQRWRMFLAVGLLGTAIPAFLFSFAQQ
                     RVSSSVAGMLNTLTPLFTLVLGIVFFQAKVIWAKVAGVILGLAGATVLILLGQKGGLS
                     GSVAYSMLIVVATVCYGANLNIVATYFGNTNSLTLSAVSFTMVGIPGLIFLLTGTDFL
                     HRLSTQAGALTGLGYVAILSLGGTVIASVLFFHLVKKTSAIFSSTVAYLMPVIALGWG
                     VLDGETMTVFHLAGIALIMAGVYLSRR"
     gene            complement(18431..18871)
                     /locus_tag="PK198_03075"
     CDS             complement(18431..18871)
                     /locus_tag="PK198_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF37748.1"
                     /translation="MSLIKYDPFAPVRSLTNVVDEFFNRGIGELVGYDHAFSVPSVNV
                     VENPQNFLLEIAAPGLEKGDFKVSVENGSLNISAKKEKKEEVQEGKYTRREFNYTSFS
                     RSFNLPEGVKPEEVQAVYENGVLKVTLPKKEVAKMEDARVILIE"
     gene            complement(18991..19452)
                     /locus_tag="PK198_03080"
     CDS             complement(18991..19452)
                     /locus_tag="PK198_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF37749.1"
                     /translation="MTSVVFNPSLKMRKPSFTPPVLDKLFDDLLRQDWPFLNVPGTDA
                     ALRPAVNILEGEADFRIELAAPGLEKTDFKVEVNKDLLTISAGKEAQPVEGVKILRRE
                     FSFLNFQRSFRLPNSVDVNGIQAGYQNGILTVTLPKRDEARVKPPVSIEIA"
     gene            complement(19477..19665)
                     /locus_tag="PK198_03085"
     CDS             complement(19477..19665)
                     /locus_tag="PK198_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37750.1"
                     /translation="MVKSSPSLPHKSVRPVQPTFCHFTTIVSATLALKTEGNLTKSPE
                     NQTGTRLAIDGLKTIKII"
     gene            19754..>21336
                     /locus_tag="PK198_03090"
     CDS             19754..>21336
                     /locus_tag="PK198_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF37751.1"
                     /translation="MKNQKKGAKEECIEVTGARVHNLRNVDVSIPRNQLVVITGISGS
                     GKSSLAFDTIYAEGQRRYMETLSAYARQFIGEMERPDVEQITGLSPVISIEQKTTGRN
                     PRSTVGTVTEIYDFLRLLFARAGEAYSYISGEKMVRYTEDQMKERIVELFDGHKIIVL
                     APLVRGRKGHYRELFEQVRKQGYTKVRIDGEIRELVPDLQVDRYKVHDIELVVDRLKV
                     GDDRRDRISTSLQTALRSGNGLVFILDTDTEKVSAFSKHLMDADNGLSYEEPSPNTFS
                     FNSPYGACPTCNGLGAINAVDMARVIPDDTKSINDIGIAPLGEVRENIMFQQLRGIAK
                     KYNFSFSTPVKDIPQEAMQIILHGGDNSFPVKVNYGDSDISYNIAVEGLLNMLKRWYE
                     NTTSEKIRQWAEDFMTVDVCPDCQGARLKKESLHFKIDEKNIAELSDMDIVTLSNWMA
                     ELEDRMDERRKVIAKEVLKEIRFRLGFLLHVGLDYLTLNRPSRTLSGGESQRIRLATQ
                     IGSQLTGITYILDEPSIGL"
     assembly_gap    21337..21400
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21401..22607
                     /locus_tag="PK198_03095"
     CDS             <21401..22607
                     /locus_tag="PK198_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF37752.1"
                     /translation="LVVEHDKDIMMASDYLIDLGPGAGKHGGLVVAEGKPKTFLKKNT
                     ITSEYLDGRRSIPVPAQRRTGNGHFLELSGATGNNLQNVQLRIPLGTFTCVTGVSGSG
                     KSSLINETLYPILRQHFYKSLQRPLAYANIVGLEHVDKVIEIDQSPIGRTPRSNPATY
                     TGVFTDIRKIFSELPESKIRGYKPGRFSFNVKGGRCEVCEGSGMRVIEMNFLPDVYVH
                     CENCLGRRYNRETLEVLYKGKSINDVLDMTVSEAVEFFQPIPAIYRKLRTLDEVGLGY
                     ITLGQQATTLSGGEAQRVKLAEELSKKDTGSTIYILDEPTTGLHFEDIRHLLDVLNKL
                     TDKGNTVVVIEHNMDVIKTADFVIDIGPEGGRRGGNIVCADTPEGVALCAKSFTGVFL
                     KEELKVPRE"
BASE COUNT         5341 a         5844 c         5866 g         5754 t
ORIGIN      
        1 gacaaccatg ggacgcatct tggaaccgaa atagtccaat ccgattttga tggcgatctt
       61 tttgcctggc acaaaatcat cttgctcgct gacgtgaaag gtgcgctggg aggcatctcc
      121 atcccggatg actattttgg cgctgggaat gcgattgatc tccttgacaa tggaaatgga
      181 catcacctca taggcggggt caatcacact accctccgag aggatgtcaa atgtaactac
      241 atcatgacga gaggggttgg gaattgaagt tgtgttgctc atacctcagg catcctccgt
      301 tttgtcaagt ggtggaaaag aaagttcgat cccaggctta atcaacctga atgtggtcag
      361 gttgttggca cgggccacct gctgtgtgaa tttggggtcg ttgtagaatt cgccgaccat
      421 tttatccaag cggtctcccg cttttatcct gcggatacgg gtaaggtcag gggattgacg
      481 ccgtgcctcc tgttctcttt gttcttgtgc gacataatcc aaaaaggtag cgctgatctt
      541 ggctctcagg ggtttcccgt tggggtggaa cagcgtgtag ttgatgtcca aattgcttag
      601 gataccctgg aacaagtgct ccccccacac gattttcagg aaatggggcc ggtgannnnn
      661 nnnnnnnnnc ccgccatatc gtaaacagta ttgagaaaac gctctatttg ctgagatacg
      721 agatcatctc ttggcacagt cctgttggtt acggttgcgt cactttcggt gcggtttttg
      781 gccaagccat cgtagtcata gccctgaatg gtgtcggtgc cgtcaaaata aaaatccagc
      841 ttgagctgtt ccggtgctgt tgaattgaat cgcccggagt tgccctggct tccatgaggg
      901 cgccgattgt cgtactccac tttgtaattt ttggagtaag actccgggtt gaccggcaga
      961 gaaatcgtac ctgcttcccc tgtgaaatcc ctgtttttga atgctttgat cttcaacttt
     1021 tccatcaacg ctccaacttt tccttgatga tttcaagcac ttttctacgc attgctgaac
     1081 gagctgctcc tcggcattcg tgctattggc accttcgccg gcacttgata ccgctccggc
     1141 attcgcgccg tcgcccgtac ctacacctgc tttaattatc aattccctga tttcgagcgg
     1201 catgatacat ttggttttac aaaatcagac attgctgaat tcaaagcgat tgtaggacaa
     1261 ctccagcgtt tcaacgagaa cttctccacg ctctgcgttc agctcaccgg ttttccagct
     1321 tttcggccag gcatgctcta ctttccactt catcagcggt ttgtgatttt catccaggag
     1381 taatacatcc aaattggccg gcctgacgat acctttttca aaagcgtctt tacaccagtc
     1441 gtagatcacc gatttttccc tgccggtcag aatccccctt ttcaaggtga gggcagagta
     1501 ctttctacgg acgggaacgg agtgttcgaa gccgttttca ccgccttctt taatggtttc
     1561 cgattctatt tgcacatcca ggccggtcac agattgaaaa cccgcgtcta tgtgtttgga
     1621 gtcaagtccg ttgaaggaga cgacgaaatg aaaatttaca tgttgccaac ggtattctgc
     1681 catttgcggt caatttgtaa aaggaagaga aagttaagca ggaaccctat cttgtggccg
     1741 gatgcagcgt actctcccta tcagccttag cttactttgc ctccaagctc aacccctcgt
     1801 gaaccaactc catggtttca atagctactt cgttgccatc tgctttcagg tcgnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaacggggt tgtgttcttc
     1921 gtcgagcatg gaaatggtgc aatcgcccct aaattctgcc tttccctcct gaaagtaaaa
     1981 ggtttttttc cagtggtcga attccttgtt atctcctttg aacatgccgc gtttgagggt
     2041 gatgttgctg tacttggtaa gacccggctg cttggttttg tgatataagg gcgcattgcc
     2101 ctcccggtat tcgattacct cggtttcaaa gtccaaaccg cttacttcgg taaaaccgaa
     2161 ctcaaagccg aaataattta cttgataatg gaatttggga agtggatatc ccccttctct
     2221 tgccatgatg aaaatgtttt tctgatgatg aataaatcag gttcacaaac aggtgaaaaa
     2281 gaacatttcg cgccttttac cacctcaatg actatttgac gcctttggcc tgttcggctg
     2341 ctttgatcgc ttcaaccaaa ctgtcatagc aggcttnnnn nnnnnncaga cggtcttttt
     2401 cctctttcgc tttgtctaat tcgcattttt tcttttcttt ttcaactttt gcgcatgtcc
     2461 attgtgcttc tgtttgagta tagtagtcgc tggaggttaa agggaaaatc gggcgtgggg
     2521 caagcgcagc cgtatgcggt tgttgatcgc atcccagttg acattcccat accttgtcat
     2581 ctttagggca gcaatctgcc caagggtatt cttcctgcgt attgcctgca ttggcgccgg
     2641 ggcctgaatc atcagtgccg gctcctccgt tgcagtcctc cggttcgttg catgttccaa
     2701 aacaggcttg caattccctc aattcgctca tcaggctgtc gcagccgtaa tcttggcaat
     2761 ctgtgaccgg tatggggcta tcgttttcat cccggcatgg aatatcgcaa agcgcatggt
     2821 atagcaggtt ggcacatttc agcgcttcta tgatcttctt catcgcgtcc aactgtagag
     2881 cggccagttc cttcagcttg gcgtcgaacg cctccagtgc ttttacgata ccccggtccc
     2941 tgatcaggcc tgggtctgtg atgcagcgga gacacttcat gatgccttcc atcatggagg
     3001 tcagtgtttc cacatccacg ttgttttcat tgcggaagac cttttctact tcgcaatata
     3061 aaatctggat ggcggtgacg gacttatcgg cgttttggca aattttctca aattgcctga
     3121 tggttgtttt ggcctctctg cataccttgt cggctaagtc tgctgttttt tcaatgccgt
     3181 cgaagtataa tttcagcctt cgttcccagg cttcaatgta tgcaaggttc tttttggcat
     3241 tttctaatcg ggcaatagcg acatccaagt ctttttttcg ggccgctttt tcgtttatcc
     3301 atttttcgat gcagtcttcg ccgggagggc aattagcatt gtcctgccat tcgcagtcaa
     3361 tgtgcaccgt atctctttct cttatttttg aacgtgccat gatattttgt tgtgtttgga
     3421 ttaaggatga aaattaatcg cagagattag gctcatattc gggcttggat ctggaaccgc
     3481 ccccgccgtc aggtgtttca cagaaacaag acatcacctc tgcgcaaatg cctggtatgg
     3541 tgtcgtcaat gaggcaatta tcctcgctac agaggaaatc aactgtaaaa gcgatgccct
     3601 caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctccaatt tttttacagt
     3661 gagctccttc accgcttttg ccaattcttc cctgtctttg gtttcatcgg cacttcggtc
     3721 ttttatatta gtatctacat ctgttttgaa ggatttcact gcatctccaa tcattttact
     3781 catatccttc agacttttta cgttggagaa agtctgaatg ccggcaattt gagcagagtc
     3841 gttgaatgtt ttggatgcct gagagcatac tgctttggaa atgtcaatca gttgttcggc
     3901 aagagcggtg aatgcttcag tggttcccga tttgggatag ccgcaagttt gttcgaagtt
     3961 gcaattgagg atacggagtt gttccctgtt gcagtcgtct ttaagctggg tgtcaagctc
     4021 acaggctttg gctttgagtt cggcggcctt gtctttgagc gttttgaggc ttttactcac
     4081 ttcctgaagc gctttagcca ggtcatcgct ttttttaatt tgctcgtcca cgtttttttg
     4141 tgctgcttcg gcaacacagg tggactgaat accgacgtag aggtcaatat tccgataaaa
     4201 tctgacagcc tcctctgctt ttaaaacgaa acaaaccttc ccgttacgat cctgtaggta
     4261 gcctttagta gtctctttcg ccgcttcggc aatgccattt ttttcggttt tgttattttg
     4321 cagatgtttt ttgtaatcat gcaaaatatc tttctttttg aggcagtcgg cgggatcgcc
     4381 ggttttcgta cccccgtttt gtatggtggt tcccatgatt ttttttgttg attaattggt
     4441 tatcgaatta ggattcaggt caaatcagga ttcggagagc atcttatggc tgaactttag
     4501 aatgatgaat tctgccgggc gcaccactgc catgcctatc tctacgatca tgcgcccctc
     4561 ccaaatatcc agttccgtca tagtctcatt caggcctact tttacgaaat aggcttcctc
     4621 tgtggacgcg cccatcaatg ccccgccccg ccattgtaag gacaggaaat tctcgatcat
     4681 tgacttgacg cgtgtccaag tattggcatc gttcggttca aatacaaatt gtattgtggc
     4741 ctttttgacc gattcctcta ccatgttgaa gtacctgcgc accgggatgt accgccattc
     4801 gttgtcgttg ccggccaaag tacgcgatcc ccacacaatg cttgggccgc gcccggtaaa
     4861 gctcctgatt acatttacgg atttcccggc gttggtgtca acgttgaggt ctctttgctc
     4921 ctcatccgtt attttaataa ccgggcggat gacaaagtcc atattgacgt tggccggcgc
     4981 tttccaaaca ccgcgctgtg tgtccacatt ggcgtatatg ccaaccatcc ccggcgaggg
     5041 cggaagctcc atgggtaaat cgcgtacctt actgcgtgcg gtaaagtata gcgcattgtt
     5101 tttactcctc agttcagcca gagtgccatt atagcttcca tctcctccgg tatgcactat
     5161 ttgaacattg ccatcgcccg gtccttcgcc tccatagtag tagtcgagta tggtttctac
     5221 attagggaaa tagactgcgc cgtacttgag ggtgttgatt tcgccaggag tctcattgcg
     5281 cagcgtttca atatttgtcc tccaatcaga cactggaagc gtcgggtcat tccaaacatc
     5341 cattacggta aagcgatcct gcattttttc acacaggtcc atggcttcgc catacagggt
     5401 gtaatagtcg ctggatgtgg tgagattttg cgcgtcggga aagacgacca aggtgatctc
     5461 gtcaattttc tcggttgtat tgaggccggc cctgagttcg gcggcgccga cgatgccggt
     5521 ggagtaatct ccaacggata ctatccaaca aggaccacct ccattggcaa aatacaattg
     5581 catggagtag tacattaaat acttggaccg gtcagcgccg attgctgcgt tcacctgaat
     5641 gggccggccg gggctttcac tgatcgtgac ggtaatactc gtctctgctt gtgccccacc
     5701 gaaaaaagtc tcatattcca gtagggaagt aatgcgcttg ggttcgttca gtaggggaag
     5761 gcccctggca tcttccgctc tttccgtgta gcctatgaac gcaggtatgg cagtctctac
     5821 ctgcgcaatg gaaggcggga gtttggatat ttcctcaata taaacccccg gtgttttata
     5881 attcataatt ttttcggttt ttaaaaggtg agtaaaaata tgtcagaata gtattgtttc
     5941 tgagcttcat catagcgtat ggaagccgat gaaggattcg gcaaaaggtg ggcgccggac
     6001 ggaagattga tgctgagggg tacgcggatt tgcgacaatc ttcgcggatg agcagtagta
     6061 aattgttgct ccggccaatt ataggaaaaa tcgggatcgc cgggaataga agaagacagc
     6121 cgttctatat ggtatcgcca gaaagtgaag cggctcataa ggcggatttc aaaaacaggc
     6181 cccctccagc gcatcgccgg cgctggggtt gtaagccctg catttagcga accatcagcg
     6241 ttgaaaagac gaaagtcggg agccgtgttt tcagtggaga tatctaccaa gccccacacg
     6301 ttcttatcgc tgcccctgag gtcgtcgctc aacacaattt gccgttgttc aaataaggtg
     6361 ccattgatga acgcgtccag cacatacact ccgtcgtgta tagggcgggc gcgaatacga
     6421 tcattttccg gagtagaagg tgaaaccggc aaatacctga aatcgaggga aaccgacgaa
     6481 cttgatggat tagaaatagc aatgcgtttt atctcattgt tatataaatc ccggatgata
     6541 aattctgcat ttgatacaat tggtatggaa ggatcaaagc ggtatctgaa tgttttgggt
     6601 aaagccactc tttcgccggc atgtgcataa gagtgccaag tgccggcggc tgtatctcca
     6661 attgcttgaa agtcaacaat ggtttccgtg gttctttggg ccgcttttat ttgatttccc
     6721 tccctaatca atccccccat ttcccaagta cggccgcttt caaattcagg tggctgagcc
     6781 gataatgaag gataaatctt tccatcctcg gttgcccgca agttggtgaa ataataagac
     6841 cccggtattg tgggcatcaa ggcatgattg gcggcattca gccattgggg gtcgcgcatg
     6901 gtgagtacaa atctgcaatt cccctgttcc aactctccaa tagggtaaaa acgcgctgaa
     6961 gccccggtgc cttctcgtct ggcttggata cctgcaaaga tgcctaaagg agtctggcgc
     7021 aaaatgattt tattgcggct gagccattgc tgtgtttcct tcgttggttc tatgtttaca
     7081 atttttcgaa ggtcatactt gtattctaat acagaggctt gtttggccaa cctgctgctt
     7141 tgaggctcat ctcgaaattc atgaaatacc cctggattat tacttgagtc aggaaaccaa
     7201 taatcaagat aatatccgtg taaaatgcgg atttcaaata agcgcctgaa tataatatca
     7261 gccatatatg gttggcgggt taagtgtttt ttatttcggc ttgaatttta tctcccggga
     7321 gcttgtgtgc ttccgatatt gttctcaatt tcctctataa gcatcccgga ccctgtttgg
     7381 cggcgatcca acatgcgtac cagacgggct ttgtacatca caaacggtat ttgttttccg
     7441 cccaatgacc cccatagatg attgatttgt tcgaaggtca tagtgtaaag attgagtatc
     7501 agttgtatat cggccaagtc cggttcattg atgagaaaac caggagcatt tctcaaatta
     7561 aatacatgtt ttccctgaaa gaactcaatc acagatgata accgtcctaa agattcccgg
     7621 tagccatccg gggtaggtgg aaaaaaagcc gtaaaaagca ggtagagatt caaataaacc
     7681 ggggggtttt catatcgaac tcctccatta attgtttttt taaaatgagg attatttttc
     7741 agtgtactct cttcctcaat atttactaag ctaacgacta ctttaccatc caagccggtt
     7801 gcattttcat tttctatttg ccctacatta ctcaattccg cataattttc agtgtttggg
     7861 cctaacctgt tttgcaggta ttcttctaat tcgtgatgga gcagatttaa tgcctggtgt
     7921 atcatagcgt atagctcatg tcattaacca ccctcttcac ctcctccccc ttcacctcca
     7981 cccgcgcaaa acactgcaag ctcggcagcg ccaattgaag ccgggcatgg ccgctaacag
     8041 acatgaccgt gccgcagtag cccgccagcg gaccggtcaa cacctctacg gtttcgccca
     8101 cgcggaaacg ctcgtaagtg atctccaccg gggcttccag ccgggccagc cttttgatca
     8161 tcgccacctc cttttcactc agtgtagcga tgcggcctcc aaactgcaca tatttgagaa
     8221 cgtgccccgc ctggaaaacc tcgttgcggc gcttgttgtc ggtggctaca aagacgtaat
     8281 tgttgaacag cacgacttcc acctttttcc ggcggtcact ccattgccgc agttgcatct
     8341 gggtgggtac gcaggcttca aagcctaatt cgccgaggcg ttggcccact tttttctctg
     8401 tgcgcggctt taccaaaaga gcataccaac tgatttcggc ggcgctgctt ggttgatgtt
     8461 gcatgatgtg gaaaataaag attgggtggt aaggtgtttt gggctacggc ttcgcccggc
     8521 tgagtcacat agtgactttg cccggcaaca aaaagaaggc cattcttcgg aataggctta
     8581 attgttttga ttcaacaaat gtaggtataa taatatttta aaaaaaatca aaacggcatc
     8641 tttacaccaa agtgtaaccc tcctcatacg atcccctccc gcacggcctt cgccactgcc
     8701 tctttgccgc aactcacatg cagctttttg taaatattgg ccaagttttt cttcacgccg
     8761 tcttccgaaa gatgcacctc gccggctatg attttgatgg gcagcccgtc caccaattgc
     8821 cgcaggatgt tcagttcccg ctcgcagagg tggtggtctg attttttcgg caacttgtga
     8881 aaatattgca ccactttttg cgcaatgcct ggcgacatgg gtgaacctcc ctccatgacc
     8941 tccgatatgg ctttggtcaa ctggtcgggc ggcgtctttt tgagcaggta gccactcgcg
     9001 ccggcgcaca gcgaatcgaa caatttttca tcgtcctcat acaccgtgta catgatgatg
     9061 tgcgtgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnngtcggcg ctgtcttcgc agatgaggat tttgaaaggc
     9301 ggcatcaggc gatgttttga gtgacaatat cggtgcaaaa ccggagcgaa gcaagtacgc
     9361 tttggtgtaa ctcacacttc ggcgggaagg tgctgtagta cggtttttgc tgccagcgga
     9421 gccagttttt ctttttcttg aggatgactc cacccccaag ccgggcgcca ccacgggagt
     9481 acgggcatac tgaattgaat atggcaaccg ttgcccggcg aagattggat gttgagttgg
     9541 cctttcatgc cgatcattcg cattttcatg ctaccgagac ctgctccgaa gtatgttttt
     9601 tcggtgttga agcctttccc attgtcctga atgctgcaat acaaacgccg gccatcaagg
     9661 ctgaaccgca cattcacttc gccggcgtcg gagtgccgca tcgcgttgtg cagggtctct
     9721 ttgatgatga ggtaaaggtg ccgcctttgt atcgcaggga ttcgtatggc cttgagcggc
     9781 ggggctaccg acagagagaa acggatgcct ttgggctcca gtagttcatg tccgtgccgg
     9841 ttgatgagag cggccaaatg ccccaattgg cgactggagc tgccaagcac cgctaccgtg
     9901 tcgctgatgg agttgtgtac ctgatcggcc tcttcactga tgcggtctaa atatttctgg
     9961 gccagttctt tatctccatt caacaactcc atggacacca atccggccag catggagata
    10021 ttgctcagcg aggcccccaa ttcgtcgtgc aggtcgcagg caatgctgct gcgcaatttt
    10081 cgcatggctt tgcgttgccg caacaaaatg aaaattagca tgcctacgga taccgttgca
    10141 aaaagcaaaa caggaagaat aatttgaggt ttcatattga ttgaatgggt tggtgtaaca
    10201 atactctcaa aactaaatag atagagacgg acaaatgtta ccggtcatag cagttcgcac
    10261 caaaaaaggt acctgaaatt tatggttcca cagaatcata aaaagcaaga cccgcgaagt
    10321 gctttcacgg caaaatcagg tcgtcttgtg ccaatatagt gaatcgggct tgtgtaaggt
    10381 ttgcttaaag agtattcgta gatagtattg caaggagaac aaatatacga tgttttttat
    10441 tgtgatgcaa cataaatatc gaaaaatcaa ctcaacgccg ctcgcagtac ctccaccggg
    10501 tgcagtgctt tgcggcccgt tccgtcggct atctggtggc ggcaactggt gcccggcgcg
    10561 gcaatgatga cctcctcgcc ggctttgcgc acggcaggga nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    10681 gccgcaacaa ccggatggga tgacctccac cgtgtagttt tttggcaatg acagtgccca
    10741 tgcagaaggc tctaccccgg ccaatgcctt ttggtggcaa tgcccgtgca gcagcactcg
    10801 cgccggcgcc tcggtaaagg cttcggcact gatgcggcct ttttgtattt ccgaagccag
    10861 aaattcttct atcagcagcg catgttgccc cagggtgatg gcggcctcgc ggtcggcggg
    10921 ttccaccaat tttggatact catcgcggaa gccgagtatg gccgagggtt cgatgccgag
    10981 caggggcgtt tccgggccga cgagttcgcg gaagatgcgc acattttcac gggccagacg
    11041 ccgggcagga atcaacaaac ccttcgagaa atgtgcccgc ccgctgtcgg gatgtggcgg
    11101 catttccacc cggtagccca gtcgggtcag tagttccacc gcctgtatgc cgacatgggt
    11161 gtcgttgtag ttggtaaact catcgcaaaa gagatacacc ttgccttttt cattgccgga
    11221 aggctggagg tggcggcccg tttgattcca ccatttgcgc agggtggttt tttgcagcaa
    11281 aggcatggag cgctgaggcg caatgtgcaa tgcccgcttg agcaggcccg aagtgagcgg
    11341 gttttttaga aaaaaattgt tgatgcccgg taccgtcgcc gccagcgcgt tgatgctgcc
    11401 gatgtgcccg aaaacccgtg cgcgcagggg cacgcctttg gcctggtaat aatggtgcaa
    11461 aaactcggct ttcatcgtgg ccatgtccac attggagggg cattccgagg cgcaaccttt
    11521 gcaggacagg cacagtgaca tgacctcgta cagttcttcg tgggcaaagc gattctctcc
    11581 gttttccgag cgggtgagaa actcgcgcag ggcgttggcg cgggcgcggg tggtgtcgcg
    11641 ttcgttgcgc gtggccatat acgaggggca catggtgccg ccggtgacgt ggggcaactt
    11701 gcggcaatcg cccgaaccgc tgcatttttc ggccagtccg aggatgcctt ttgtggccga
    11761 gaaatcgaaa acagtatcaa tttttggggg cggcgcgtct tgcaggtagc gcagcgaggt
    11821 gtccatcggc agggcatcca cgattttgcc gggattgaaa atgccggcgg ggtcccattg
    11881 ctgtttgatg cggcgcaaga gggcgtaatt gtcctctccg atgatgagcg gaataaattc
    11941 ggcgcgcacc ctgccgtcgc cgtgttcgcc gctgagcgag ccattgtatt ttttgatcaa
    12001 tcgggcggaa gcctccgcta tttcccgcaa ttgtctgacg ccctcggagg ttttgaggtc
    12061 gaggctgggg cgcgggtgta gttcgccggc gccggcatgg gcgtaataca cgcattgctg
    12121 accaaaagag gccatcatat cggcaaaatc ggcgatgtat tcgggcaggt cggcgatgtc
    12181 cacagcggcg tcttccacaa attcgagtgg cttggcgtcg gttgtcacat tcgacaacac
    12241 gccgaaaccc gcagcccgca aggcaaacac ctgctgcgac tgtgcccccg aaacgatggg
    12301 gaacgcatag cccagaccgg cagcgcggag atcggcaatc atggcattgg cctctgcttc
    12361 ggcctcctca aaagtttgtc cccggaattc caccatgagg atggcggcgg gatcgccctg
    12421 caaaaagaag cggtttttct gttgcgagcg gttggctttg gtacaatcca agatgaactt
    12481 gtccatcagt tcgcaggcat aaggggcgcg gcgcatggcg atttgggcag cccgaagtgc
    12541 ggcgtccaac gtgtcgaaat gggcgcatac caaagcatcc tgcggtggcg ggaggggact
    12601 cagattcaat ttgatttcgg tgctgaatgc gagggtgcct tcnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggactgaaaa tattggattg taacagcacg
    12721 tccaccgcat agccggtatt gcggcgcggt atgccgggtt tgggatattg ttcgcgaatg
    12781 gaggtctggc gttcgggctg cgacaactcc tgtgaaatct gtcggtagag atcgccttcg
    12841 aggccgggcg cggctgattt tttttcaaaa tcttctttcg acaatggccc gaacgtgacc
    12901 gtatcgccgt cgctcagcac ggcgcggatt tccagcagat ggtcgcgggt ggtaccgtat
    12961 ttgatggaag tactcccgca ggagttgttg cccgtcatgc cgccgatcat gcagcgcgag
    13021 gcggtggcgg tgttgggacc gaaaaagagg ccgtggggtt tcaaaaaagc gttcagttca
    13081 tcgcgtatga cgccgggctg cacgcgcacc caggattcgg cgacgttgag ttccaaaata
    13141 cccgtcatgt gccgcgacac gtcggccaca atgcccgtgc ccacgcactg tccggccaaa
    13201 gaagtgcccg ctgtacgcgg aatgatggag acgccttccc ggcgggcgaa ggcgatcaaa
    13261 agtcttatgt cgtcctcatt tttaggataa gctacggcta atggtatttc gcggtagatg
    13321 ctgccgtcgg tggcgtagag cgtgcgcagg aggcggccgg tatgtagttc gccggtgagt
    13381 tggcctgcca atcgggagag ttggtcttgc atgattgctg aagctgtgat gtgggacaaa
    13441 gttcgcaaaa aaaatcaggt tctcacaagg aataccaggt tccctaagct gaaattccga
    13501 ccaatatttt acgccataca gaataagtcc ttaccttagc ccaccccgtt cataaactgc
    13561 tgcaccaagc ggtgaaaatg atgcaagccc tgctcctgtg tgggtgagta gcgcccggtt
    13621 tggtagtacc gcgactgaat gccccgctgc acgctttcca ccaccgcttc atcttccatt
    13681 tccgtgagat ccaactgatt gagtgcacgc tcagaggctg cattgtcgaa ataataggtt
    13741 ctgaaccgga tgcgggtgcg ctgatgcgag aggggttcta cgacgttgag cgacaagccc
    13801 caggggtaaa aattgaacat caggttggga aacacccagt agtagtaggc atacacccgg
    13861 cggccatgat cgggcgcgcc ggcaggaatg tcgaagcagg gttcaccctc tttggcaata
    13921 cccacttgca ggttgcagta cgggtgcaat tcatactcgt attttccgaa ttcaatggcc
    13981 tgattcagcg ccggatgcac aaacggaatg tgaaatccct ctaagtaatt gtcgcaatac
    14041 aaagcccagt tggcctgcac ctcatagtct tgcgagtgtt ccggtgaata gcgcaaggta
    14101 tccaatgcca tccagccgat gcgctctgtg atggggcgta tcaattcctc aaacggcatg
    14161 gcgggctgca acgacacaaa caacagcccc agccattcgg caaacggtat ccggctgagg
    14221 tcgtcggcag gggaggggaa gttctccacg ccatcgaact cgggcatact tcggaaacgg
    14281 ccatccaacc tgaaacagcg cccgtggtag ccgcaagaca gaatgcgttg ggatccaggc
    14341 tgttctacca ccaatttccc ccggtgtgta cacacattgg aaagacaatg cgcccgtcct
    14401 tctgcgtcgc gggtgaagag cagcggctcg tccaatacgc cgggcaagag cgcaaacggc
    14461 cacacttgct gcggcgatgc aatgacttcg ctttcggcca cccactgcca ggtggaggcg
    14521 aaaatgcact ctttgacggt ttcaaaagtg cctgcctccc ggtagaaagc ggccggcagc
    14581 gtgtgcgcct gatggatgtt ttcggaaatg tgcataacaa acaatgtgct gatgtgctaa
    14641 tgtgctgcgt ccaatctcaa gttttcacag cggttgcccg ctcttttcca tggcggccct
    14701 gcgctgctcc acatattgcc gccactcggt aagggcttcc tgaaaatcag ccggtagttc
    14761 gctgtcgaag tacatagcct cgccggtggt gggatgtatg aagcccagcg atttggcgtg
    14821 cagcgcgtgc cggggcatga tgttgaaagc cttttgcaca aacannnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnntgtac ttgctgaaca ccgtgccttt cacaatgtaa tcgccgccat
    14941 agcgctcatc gctgaacagc gggtgcccga tgtgtttcat gtgtacgcga atctggtggg
    15001 tgcggccggt ttcgagattg cattgtatga ggctcacaaa atacagtcgt tccagcactt
    15061 tgtaatgcgt gacggcccat ttgccatgat cgccttccgg gaaaacggta aacatctgcc
    15121 ggtctttgcg gtcgcggccc acatgcccgc gcacagtacc ctggtcggct ttgggttcgc
    15181 cccaaaccat tgcgtaataa gtgcgctgta cggtgtggta aaaaaactgt ttggccaagt
    15241 gtgacagcgc aaactctgtt ttggccacca ccatcagccc ggaggtgttt ttgtcaatgc
    15301 ggtgtaccag gcccacccgg ttgtcttgcc cgtcgccgat gacgggggcc ttattttgct
    15361 ggaaatgcca tgccaatgcg ttcaccaaag tgccccgcca atgccctacg cccggatgca
    15421 ccaccaagcc cgccggtttg tgcagtacca ataggtcgtc atcttcatag atgatgtcga
    15481 ggggcatgcg ctgcggcaca atgcgaacgc cgctgccggg cggtttgggt accagcacgg
    15541 tgatgcgctg gcctggtttt actttgaaat tgggcttgat ttcctgattg tccacgcgga
    15601 tagaaccggc tcgaatggca ttttgcaggc gattgcgaga cacacgttgc attctgtcta
    15661 tcaggaactt atcaatgcgg atggggcttt ggttggggtc caccaaaatc tcattatagt
    15721 ccgaatactc ttcatcctgt tgttcggagc cttccaattc ttcttcctct tcctgcatgt
    15781 ttcgttttca ggtgtacttg cgatgtgcaa aggtatcaaa aaattaagaa tgagacaccg
    15841 ccgccagcac gccgaaataa tggcacacgc tgcccgccag taccagcaag tgccagattg
    15901 catggtggta aggcagcttt ttggccaaat agaagaccac gccgagtgtg taacaaactc
    15961 cgccggcaag aatccaggcc agaatgtctc ccggtaccat cgcaatcata ggcttgatga
    16021 tgagtacggc agcccagccc atagccagat acagcatggt ggaaatgaac ttgtgtttgc
    16081 cggtggcgaa tattttgtac accaacccga tcagcgacac gctccacaac ccgatgagca
    16141 cgccgtagcc ggaaaaagtg ggaacgaaca acaaaatgaa gggcgtgtag gtgcccgcaa
    16201 tgagaaagta aatggcaatg tgatccaatg tattgaagac gcgacgcagg cggggcctgt
    16261 tggcggcatg gtacaaggtg gaggtgagat aaaccaaaat gagcgaaacg ccgaatgcca
    16321 gaccgcccgt taccaagctc cagtgcaggg tttgtacgaa aattttgacc aacaaatagg
    16381 aggttccgcc aatggccagc agcagcccca tagcgtgtgt gacgacatta agaagctcct
    16441 ctttcggcga ggcgagatcg gcagcgacga tttggtaata gcgatgaagt ttcatattga
    16501 tgcagatgat tagatgaata tcggcaggct tttgcaagcc gcaagcctgg cgtttagtca
    16561 acagttgacg ccggcgctcg gttgccgcat aactaacgct gttttgctct caatctatat
    16621 tttaaatacc tcggccagtt gaaaacacta actttgctca taaatcttag ccacatggca
    16681 ctcatcaaat cagttcgggg cttcacaccc caaaatggcg aggactgcta cttcgcagaa
    16741 aatgccacca tcatcggcga cgtggtcatg ggcgaccaat gcagcgtttg gtttcaggcg
    16801 gttgttcgcg gcgatgtcaa cagcatccgc atgggcaaca aagtcaatat tcaggatggc
    16861 gcggtcattc acggcaccta cctgcgggcc gccaccacca tcggcaacaa cgtctccatc
    16921 ggccaccggg ccatcgttca cggctgcacc ctgcacgaca atgtgctggt gggcatgggc
    16981 gccattatca tggacaatgc agtggtggag gaaaatgtac tgattgcagc aggtgcggtg
    17041 gtattggaaa acagccgcct cgaatcgggg catatttatg ccggcgtgcc cgccaaaaaa
    17101 gtgaaggccg tcagtccgga gcagttcaaa gacagcatcg agcgcatcgc caatgcctac
    17161 atcatgtact ccggttggtt caaggacgaa gacgctccta acgccggctg agctgatcgt
    17221 ttcaaatgcg tagtgaaggg gtgactatct ccgcgtttnn nnnnnnnnnn nnnnnnnnnn
    17281 ccttcactca accaatatcc atccctccta acgccggctg agatatactc ccgccatgat
    17341 gagcgctatg cctgccaggt gaaaaacggt catggtttct ccgtccaaaa cgccccagcc
    17401 caaagctatg acgggcataa ggtaggccac cgtggacgaa aaaatggcgc tggttttttt
    17461 taccaaatga aaaaacaaca cggaggcaat caccgtgccg cccagcgata aaatggccac
    17521 atagcctaag cccgtgagcg ctccggcctg cgtgcttaaa cggtgtagaa agtcggtgcc
    17581 tgtcagcaag aatatcaggc ccggaatacc gaccatcgta aaggatacgg cgctcagtgt
    17641 cagggaattg gtgttgccga aataagtggc tacaatgttc aggttggcgc cgtagcacac
    17701 cgtagctacc acgatgagca tactgtaggc cacgctgccc gacagcccgc ctttttgccc
    17761 cagcaaaatc aacacagtag cgcccgccag ccccaggatg acgccggcca ctttggccca
    17821 gattactttt gcctgaaaaa agacaatgcc cagcacgagc gtaaacaaag gcgtcagtgt
    17881 gttcaacatg ccggccaccg agctgctcac ccgctgttgg gcaaaagaga acaaaaacgc
    17941 gggtatggct gtacccaaca gtcctactgc cagaaacatg cgccaacgct gccactctac
    18001 ctcacggcgg cgcgcccaca ccaccggaaa taaagccaat gccgtgatac tcagccgcaa
    18061 acaagctact tgtgtggcac tgtaggccgt caaactcttc ttgatgagga tgaaagaact
    18121 gccccaggtg agggccaaaa tgccgagtat gatgaatccc aaccagcggt cggaaatgcg
    18181 cggcgcagcg gcgggcgccg gtttggtcgt agtgctcatg gcctgtgttg actttggcaa
    18241 accggctaca tagccggaat acgcgtaaac gctcggattc ggattccggt tgcagacaat
    18301 ccaatgcccg gcacataaaa aagggtcggt accgaaaagg ataccgaccc gtcaaaaaca
    18361 cctaaaaccc tattaactaa cctttgcacc tgcccggagg caggattttc attaacccca
    18421 aaactgaggc ttattcgatt aagatgacgc gggcgtcttc cattttggct acctcttttt
    18481 tgggcagcgt cactttcagc acgccgtttt catacactgc ctgtacttct tcgggtttaa
    18541 cgccttccgg cagattgaag gagcggctga acgaagtgta gttgaattcg cgcctcgtgt
    18601 atttaccttc ctgcacttct tctttctttt ctttcttggc agagatattg aggctgccgt
    18661 tttcaacact cactttgaag tcgcctttct caagtcctgg agctgcgatt tcgagtagaa
    18721 aattttgcgg gttctctact acgttcacag aaggcacgct gaaggcatgg tcgtagccca
    18781 ccaactcgcc gatgcccctg ttgaaaaatt cgtccactac attcgtaaga gagcggaccg
    18841 gagcgaatgg atcatatttg atgagtgaca taatcctgaa ttttaaaagt taaacaatga
    18901 ttgagcggtt tttactgcgc gataacagtc acgagaatgt catttttgtc tcgttgtgcc
    18961 ggctgcaagc tacaggctgc aagccgcaag ctatgcgatc tcgatgctga ccggcggttt
    19021 tacgcgtgct tcatcgcgtt tgggtagcgt gacggtgagg atgccgtttt ggtagccggc
    19081 ctgaatgccg ttgacgtcca ctgaattggg caggcggaaa ctgcgctgga agttgaggaa
    19141 actgaactcg cggcgtagga tttttacgcc ttccacgggc tgcgcttctt tgccggcaga
    19201 aatggtgagg aggtctttgt tgacttccac tttgaaatcg gttttttcca aacccggcgc
    19261 ggccagttcg atgcggaaat cggcctcgcc ttccagaata ttgacggcag gacgcagggc
    19321 tgcatcggtg ccgggcacat tcagaaacgg ccagtcctga cggagcaggt cgtcgaataa
    19381 tttatcaagt acgggcggcg tgaaggaagg tttgcgcatt ttcaagctcg gattgaacac
    19441 tacagatgtc atggtttttt gaattttgat ggttgatcaa atgattttta ttgttttcaa
    19501 accatctatt gcaaggcgcg taccagtctg gttttcagga ctttttgtca gattgccttc
    19561 tgtttttaaa gccaaagtgg cagaaacgat tgttgtaaaa tgacaaaaag tcggctgcac
    19621 ggggcgaacc gacttgtgag gtaaggacgg agaagatttt accaacaaat agttgtttaa
    19681 atttgcttcc gcgcaaccgc cattctctat ttttgtttac tcaaactatg gccgctataa
    19741 acactgccta cgcatgaaaa accaaaaaaa aggcgcaaag gaagaatgta ttgaagtaac
    19801 cggcgcgcgg gtacacaacc tgcgcaatgt ggacgtgagc attccccgta atcaactcgt
    19861 cgtcatcacc ggcatcagcg gcagcggcaa gtcctcgctg gccttcgata ccatttatgc
    19921 cgaggggcaa cgccgctaca tggaaaccct ttcggcgtat gcccgccagt tcatcggcga
    19981 gatggaacgc cctgatgtag agcagattac cggcctcagt cccgttattt ccatcgaaca
    20041 aaaaaccacc ggtcgcaatc cgcgctccac ggtgggcacc gtcactgaaa tatacgattt
    20101 cctgcgcctg ctctttgccc gcgccggcga ggcgtactct tacatcagcg gcgaaaaaat
    20161 ggtgcgctac accgaagacc agatgaaaga gcgcattgtg gaactctttg acggccataa
    20221 aatcattgtg ctggcgcctt tggtgcgggg gcgcaaaggc cactaccgcg aactcttcga
    20281 gcaggtgcgc aaacagggct ataccaaagt gcgcattgac ggcgaaatcc gcgagctggt
    20341 gcccgatttg caggtggacc gctacaaagt acacgacatc gaattggtag tggaccgcct
    20401 caaagtgggc gacgaccgcc gcgaccgcat ctccacctcg ctgcaaaccg ccctccgctc
    20461 cggcaacggc ctcgtcttta ttctcgatac cgataccgaa aaagtctccg ccttcagcaa
    20521 gcacctcatg gatgccgaca acggcctgtc ttacgaagag ccttcgccca ataccttctc
    20581 cttcaactcg ccctacggcg cctgccccac ctgcaacgga ctcggcgcca tcaatgcggt
    20641 ggacatggcc cgcgtcattc ccgatgatac caaaagcatc aacgacatcg gcattgctcc
    20701 gctgggcgaa gtgcgcgaaa acattatgtt tcagcaactg cgcggcattg ccaaaaaata
    20761 caatttcagc ttttccacgc ccgtcaaaga tatcccgcag gaggccatgc agatcatcct
    20821 gcacggcggc gacaactcct ttccggtgaa ggtcaattac ggcgattccg acatctcgta
    20881 caacattgcc gtggagggcc tcctcaacat gctcaaacgc tggtacgaaa acacgacttc
    20941 agaaaaaatc cgccagtggg ccgaagactt tatgaccgtt gatgtatgcc ccgattgcca
    21001 gggcgcccgc ctcaaaaagg aatccttgca ctttaaaata gacgaaaaaa acatcgccga
    21061 actctcggat atggacatcg tcaccttgtc caactggatg gcggagttgg aagaccgtat
    21121 ggacgagcgc cgcaaagtca ttgccaaaga agtactcaag gaaatccgtt ttcgcctcgg
    21181 ctttttgctg cacgtcggcc ttgattatct tacactcaac cgcccttcgc gcaccctctc
    21241 cggcggcgaa tcgcagcgca ttcgcctcgc tacccagatc ggctcgcaac tcaccggcat
    21301 cacctacatc ctcgacgagc cgagcatcgg cctgcannnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attggtggta gagcacgaca
    21421 aagacatcat gatggcctcc gactatctca ttgacctggg gccgggcgca ggcaaacacg
    21481 gcggcttggt ggtggcagag ggcaagccca aaactttttt gaaaaaaaac accatcacct
    21541 ctgaatattt ggacggcagg cgctccattc ccgtcccggc gcagcgtcgt accggcaatg
    21601 gccattttct ggaattgagc ggagctaccg gcaacaatct ccaaaacgta cagctccgca
    21661 ttccgctggg tactttcacc tgtgtcaccg gggtgtcggg aagcggcaaa tcttcgctca
    21721 tcaacgagac cttgtacccc attctgcggc aacattttta caaaagcctc caacggccgc
    21781 tcgcctacgc aaacattgtc ggactggagc atgtggacaa ggtcatcgag attgaccaat
    21841 cacccatagg ccgcacgccg cgctccaatc cggctactta caccggcgtt tttaccgaca
    21901 ttcgcaagat tttttcagag ctgccggagt ccaaaatccg gggctacaag cccggccgtt
    21961 tctccttcaa cgtcaaagga ggccgctgcg aagtgtgcga gggttcgggt atgcgcgtca
    22021 ttgaaatgaa cttcctgccc gacgtgtatg tacactgcga aaactgcctg ggccgccgct
    22081 acaatcgcga aacgctggag gtgctgtaca aaggcaaatc tatcaacgat gtgctggaca
    22141 tgaccgtgag cgaagcggtg gaattcttcc aacccatacc ggccatctat cgcaaactgc
    22201 gcaccctcga cgaagtaggg ctgggctaca tcacgctggg acaacaagcc accaccttgt
    22261 cggggggcga ggcgcagcgg gtgaaattgg ccgaagaatt atccaaaaaa gacacaggca
    22321 gcaccattta catcctcgac gaacccacca ccggcctgca cttcgaagac atccgacatc
    22381 tgctggacgt gctgaataaa ctcaccgaca aaggcaatac cgtcgtcgtc atcgaacaca
    22441 acatggatgt catcaaaaca gccgattttg tgatagacat cgggccagaa ggcggccggc
    22501 gcggcggcaa tatcgtatgc gcggacacgc cggagggagt ggcgctgtgc gccaaaagct
    22561 tcacgggagt atttttgaaa gaggagttga aagtaccgag ggaataagcc tgtattgaaa
    22621 aaacaatgag gcccgaatgc cattgaaggt attcgggcct tttcttcttt cgggcaatcc
    22681 aattgcgccg attcgtgcga tcttaaaaag gtccgggggg aaagaccact gtgtcttttg
    22741 aaggatatgg atttaataca atacccttct tcccggtttt tgcgtgagca tgttggcggc
    22801 agtgattacg cccagaccta ctgtgaatgc acccaaaaca atgtaaagaa tcatcatagc
    22861 tcaacgtatt taatcggtta gtgaatacaa gcatcatgcc acttccatac ggaaaggcaa
    22921 acaaaacctt gctcaagtgt tgttgagata aaggagacaa tcgtgcagga aaatatccgg
    22981 cagagttgct gctacgacaa tatgttcggg tgggaaaaat atgggttttt gggataggaa
    23041 ctcctaagag aacagaaatt ccagtgcctg aaaggcttta cgcaaatgaa ttaaaaactt
    23101 gccttacgac gaaagttctc attcataaag atgatgcctc cacctacgag gaaggctgct
    23161 gaaacgatgt aaataataag catgacccga tgtttttgaa gttgttacca aacaattcat
    23221 tgacttcgtt catgggagac gctaaggtaa atagtttttt cagggggcca aaaaaaaaga
    23281 gaaaaaaaat aaaaaaaata aaatacagag aagtatcccg gctattgggg ccgggatact
    23341 tcatggttat at
//
LOCUS       DAORLF010000019        23230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1215_length_23230_cov_2.028091, whole genome shotgun sequence.
ACCESSION   DAORLF010000019 DAORLF010000000
VERSION     DAORLF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23230
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_23230_cov_2.028091"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK198_03100"
     CDS             complement(<1..686)
                     /locus_tag="PK198_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRF37753.1"
                     /translation="MEQFLNYKILSIGSFSLSVYHLIGVAVIIAITSLLLWVLRKSIY
                     RSTRFSEGKKYAFYQVLKYVIIVIAISVGLHFVGVNVTVFVAGSAALLVGLGLGVQNL
                     FNDFISGIIILLDSTVKVGDVIEVNGIVGKVMEIELRTTTVLTRDDTYIILPNSRITG
                     NDLSNWTHEHVTSRFDINVGVDYSSDIDLVMELLRQAAQEHKAVKSDKKPFVRLLDYA
                     DSAVVFKLLF"
     gene            complement(694..>1434)
                     /locus_tag="PK198_03105"
     CDS             complement(694..>1434)
                     /locus_tag="PK198_03105"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRF37754.1"
                     /translation="AVTRSAEVFPHDRIGEITIIAAVSGVAGAKVFSILEEPQQFFND
                     PAGTLFSGAGLTIYGGLIFGFVAVWLYLRKKQIPVAHVMDAVAPALIVAYGVGRLGCH
                     FSGDGDWGIAAGTQPGWWFLPDWLWSMDYPRNIINSGVAMDVCDGRYCRRLAEPVFPT
                     SIYEAAMAFGIGGILWALRKRVKVPAMLFFIYLIFNGTERFLIEKIRVNERYDALGFH
                     FTQAELIAVLIFTAGVAGTLWLALKTKR"
     assembly_gap    1435..1648
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1649..1844)
                     /locus_tag="PK198_03110"
     CDS             complement(<1649..1844)
                     /locus_tag="PK198_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37755.1"
                     /translation="MYPDLSYVFHDLIGTPADNWLSIFKTYGLFLVLAVLSAAVILQA
                     ELRRKSREGLLLPHTETLTEG"
     gene            complement(1855..4992)
                     /locus_tag="PK198_03115"
     CDS             complement(1855..4992)
                     /locus_tag="PK198_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF37756.1"
                     /translation="MLSEKVKTYTKLAGRNDETLHQINLVKEMQTQLNQTWRPRRLNL
                     ILAITAVAGFLMAAGLPQYPSTGLKAGQNPDEDRWVDSMFNVLTPDERLGQLFMIRAH
                     SDKGADHIAGVEKLIREYHVGALCFFQGTPVKQVELTNRYQALTRHIPLMVAIDGEWG
                     LGMRLKENTISYPRQLPLGAIQDNWLIYEFGKEVARQCRRIGIHVNFAPVADVNNNAA
                     NPVINTRSFGEDRYNVAVKSNMYAKGMQDNGVMACAKHFPGHGDTDVDSHLDLPSITH
                     DRARLDSIELFPFRELARQGVGSFMIAHLNVPALDTGMNRPTTLSRYTVTELLRKEMG
                     FEGLAFTDALEMKGVTKHFKPGEVEAEALLAGNDMLVLPESMEAAFREIKRYIAEGRI
                     AQAEVNEHVKRVLRAKYRLGLTTFAPISTDSLDQQLNSPDAIALKWKLYEHSLTLARN
                     KGNLLPIKKMSTDMAALSIGASAKSKFQARLESYKKMPLFQSPKEISSSERSRLLQTL
                     GKKELVIVSLHGMSSFASQGYGLSKSALDFLETLARRTQVVLVVFGTPYSLRHFDQID
                     PVLVAYEDDPIAQDLAAQALFGAIGISGRLPVTASPLSKFNTGVTTSPVFKMGYNLPE
                     RVGMSTAAFEKMDRLAQEDIAVGATPGCVVLAAKDGQIVYHKAFGHHTYSKTQATQAD
                     DVYDLASVTKIAGGTLALMKLYDDGLVDLETPIVKYLPELAGSNKADLLLRDILAHRA
                     GLQPWLPFYRKTVEKTPEEVVVKDHYYCPDAIDEYDIPVASNLYLRRDYADSVWQEII
                     ASPLLPTRGYRYSDLGFYIVARMVHRLSGKTLDAYLEDNFFCPMGLESIGYNPWQRVE
                     LSRIAPTEYDAYFRMQKIQGYVHDMGAAMLGGVSGHAGLFANAEDLAAVLQMLLNYGY
                     YGGQRYLDPATVKTFAARHPASTRRGMGFDMRQMDARMHQNVTTRMSNEAFGHDGFTG
                     TSAWADPETGTIFILLSNRTYPSQKNSKLSSQRFRQRMQSVVYQSLKAAPDAEEWAQA
                     KIPPMEDGGIEEGNQ"
     gene            5046..6005
                     /locus_tag="PK198_03120"
     CDS             5046..6005
                     /locus_tag="PK198_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRF37757.1"
                     /translation="MLTYILFALGFVALVKGADWLVTGSSAIAKRLGISDFVIGLTIV
                     SVGTSAPELIVNVLASINGNAGIAIGNVLGSNIANVLLILGISAIIYGLPIQRNTVLS
                     EIPFSLAAALLIGFLANTDIFGLEHNEHGLGRIDGAIILFFFGLFLMYVFTLPSAKED
                     SPDQAIQAMSGAKAGLLTVIGIVALFLGGKWVVDGAVQLAQLFGMSEAFIGLTVVAIG
                     TSLPELVTSAMAAYRRNTDIAVGNVVGSNIFNLLLILGLSSVIRPLPFQQSSNIDLLV
                     VVLSGALIILALALGRRLVIKRYDGVIFVLCYVGYIAYLLQRG"
     gene            complement(6013..6831)
                     /gene="panC"
                     /locus_tag="PK198_03125"
     CDS             complement(6013..6831)
                     /gene="panC"
                     /locus_tag="PK198_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRF37758.1"
                     /translation="MSRYTSMAPHTSVGFVPTMGALHAGHLALIRRAVAAGDLATASI
                     FVNPTQFNDPEDLRKYPRTPEADVSILAKAGCHAVLMPSVEEVYPAGGQSYEFSFGHL
                     ETVMEGAFRPGHFKGVAQVVARLLDMVRPQRLYMGQKDFQQTAIVAEMLRQLNSSTQL
                     IVAETVRETDGLAMSSRNVRLSPEFRAAAPVIYQCLLDAKRLYPEMDAAAITARAMQK
                     MESAGLRPEYFEIVDGRTLLPVGNPRQADFVVACTAAWAGDVRLIDNVVLRAAG"
     gene            7041..7865
                     /locus_tag="PK198_03130"
     CDS             7041..7865
                     /locus_tag="PK198_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRF37759.1"
                     /translation="MSKKKVLIVTQEMSPFTDPSELADFTRTLPQFVQDKGMELRVLM
                     PKFGTINERRHRLHEVVRLSGMNIIVDDDDYPLIIKVASMPEARIQVYFLDNDDFFRR
                     KHVFQDEEGQIYEDNVDRMVFFCKGVLETVRKFGWAPDIVHCHGWMTSLIPAYLKTAY
                     KTEPLFQHSKIVYSLYNDELEEKMPENFFTKAAINNLHAEDLHAYQNGAGITLHKGAA
                     AFADGVVVGSPTLSDVAQATLADTDKPVLEFSEDAALPGIVEFYNSLMPEEVEAQE"
     gene            7970..9343
                     /locus_tag="PK198_03135"
     CDS             7970..9343
                     /locus_tag="PK198_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRF37760.1"
                     /translation="MKKPILLLWLALGGLFIAGSYSGCTDLTDLGADLLDEDRLGLDY
                     VDTVSIIATTVTVDSVLTQSGTSLANQLDRFMFGDYNDPVMGRVTAGVYVQPLLDRDR
                     LSLFAIPRPPSYKDAILDSIVLVLTLDSAGFYGKPSQPFGLEVLRVIEDMNASANYYS
                     NVNFATSAVPLGSAQFSPTIDSITVLDYTSLGIDTLRYPHLRIPLNMALGQELIGLDS
                     LNYLSDTTFLNIFKGLYLRPTQQSDGLVAFNWSSLRPGIFLYYTNASGLPRQYQYEIN
                     EFSSRTSRIRHDYAGSPVAPFLNDPERGKNLLFAQAMAGTNIKIEIPHAASLRNAIIN
                     HAELEIPVIAFSGDDSLKYVNNPDVYLTVPDTTLAPNPISDFTFAGSNRIPIFGGVFI
                     RGTDGKPDRYRMNVSSQLSNMAQGRVGNVMYLQIINKAQRGGRIVFAGPENTQHKMRL
                     KVAFTKR"
     gene            9347..>9739
                     /locus_tag="PK198_03140"
     CDS             9347..>9739
                     /locus_tag="PK198_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRF37761.1"
                     /translation="MCGIVGYIGSREAWPIIIKGLQRLEYRGYDSAGAALLHNGDLKV
                     IKKKGKVADLTAAAASWDVTAQQGIGHTRWATHGPPDDINAHPHTSGNKRLALVHNGI
                     IENYALLKKALQKEGHVFEGQTDTEVLVH"
     assembly_gap    9740..9755
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9760..>10173
                     /locus_tag="PK198_03145"
     CDS             9760..>10173
                     /locus_tag="PK198_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRF37762.1"
                     /translation="MRETLPIEEAVRIALTRVTGAYAIVVMDRNDPGKIVAARKASPL
                     VVGIGEDEFFLASDATPIVEHTKRVIYLNDEEIAIVRLGHEIEIRTIDNRMQTPFIQE
                     LDLEIEQLEKGGYEHFMLKEIFEQPETIADAMRGRL"
     assembly_gap    10174..10340
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10341..>10723
                     /locus_tag="PK198_03150"
     CDS             <10341..>10723
                     /locus_tag="PK198_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRF37763.1"
                     /translation="YASELRYRNPILGEDDVVIAISQSGETADTLAAVELAREKGALV
                     YGIVNVVGSSIARLTHCGSYIHAGPEIGVASTKAFTGQVTLLSLMALVLAHKRGTISE
                     SYFRRLLIELAEIPQKVSQVLESVQA"
     assembly_gap    10724..10749
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10750..11218
                     /locus_tag="PK198_03155"
     CDS             <10750..11218
                     /locus_tag="PK198_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRF37764.1"
                     /translation="IEYIAGEIKDRSNAIYLGRGYNFPVALEGALKLKEISYIHAEGY
                     PAAEMKHGPIALIDEHMPVIVIATNKSAYDKIVSNVQEVIARKGMVIAVVEEGDQIIS
                     KMADYVIEIPATEEPFTPLLSVVPLQLLSYHIAVMRGCDVDQPRNLAKSVTVE"
     gene            11249..11917
                     /locus_tag="PK198_03160"
     CDS             11249..11917
                     /locus_tag="PK198_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRF37765.1"
                     /translation="MRKMDIEYNTSRNELVMPEYGRHVQLMVEEAKRLTDNAHRQAFV
                     EKIVDLMYIMNPQQREVEDYRDRLWKHVFRIAGYDLEGVMPPNGIIPTPENDIKRPDH
                     IEYPGTQLRFRHYGHYIQELIKKAKQMEDGPIKEGLVASIGSYMKLAYKTWNKDHYVS
                     DDVIKNDLVTLSGGILSMPDEMAIENLTPMGPPKKSSPQNQRGQQQQGGGKSNKKKKV
                     WKRK"
     gene            11927..>12286
                     /locus_tag="PK198_03165"
     CDS             11927..>12286
                     /locus_tag="PK198_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRF37766.1"
                     /translation="MTLDAFEVHGGQPLSGELHPQGAKNEALQVICAVLLSAEKVTIS
                     NIPAIRDVVLLIELLQGLGVKTTQIAPDTYTFQADEVNIDYIYSEDYYKKGGRIRGSV
                     MLLGPLLARFGKALLPKP"
     assembly_gap    12287..12296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12297..13232
                     /locus_tag="PK198_03170"
     CDS             <12297..13232
                     /locus_tag="PK198_03170"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRF37767.1"
                     /translation="GGDKIGRRRLDTHLLGLETLGAHFDYDPDRSLYRIDATQMRGAC
                     LLMDEISVTGTANVVMAAVLTPGVTTIYNAACEPYVQQLCKMLNQMGAHISGVGSNLL
                     HIEGVESLHGAEHRLLPDMIEVGSFIGLAEITIKNAQIQELGIIPRIFERMGIQMEFR
                     GDDIYVPAQQEYEIQSFIDGSILTVYDAPWPGFTPDLMSIVLVTAIQAKGSVLVHQKM
                     FESRLFFVDKLIDMGAQIILCDPHRATVIGLNRRFPLRGIEMTSPDIRAGVALLIAAL
                     SAKGKSTIYNINQIDRGYQQIDERLRAIGAQIKRV"
     gene            13229..13534
                     /locus_tag="PK198_03175"
     CDS             13229..13534
                     /locus_tag="PK198_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRF37768.1"
                     /translation="MIIYNVTVKIEHDLHEDWLNWMRHTHIPDVLATGLFTGHRISKV
                     LGDDDAHGVTYAIQYECPDLDTFLQYQQQHAARLQADHARRYQDKYVAFRTLLEVVG"
     gene            complement(13483..13698)
                     /locus_tag="PK198_03180"
     CDS             complement(13483..13698)
                     /locus_tag="PK198_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37769.1"
                     /translation="MLRKPGIAATLPVLYNAEEAKRISKMFRTKLITGPGATHVTVSA
                     LGLIVLPNIGHYPTTSSKVLNATYLSW"
     gene            13840..14661
                     /locus_tag="PK198_03185"
     CDS             13840..14661
                     /locus_tag="PK198_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF37770.1"
                     /translation="MQFVDIKNDIAFRKIFGNENKKIILISFLNAVMKLKGKDSIEDV
                     EILNPYQLPIIKNLKASIIDVKARDKKGKTYIIEMQVAEPDGLDKRLLYYAGKEYSQQ
                     IESGEFYTQLKPVIFIGIFDFKFTEGDKYLSHHAVCDVENGERIIKDMDFYFVELPKF
                     KKSLSELQEVTDKWIFFIKEAENLEVVPENVDDEGLKAAYHDANKHSWTKEELEAYDY
                     AAMREQDERGKVALAEKRADKKAKNEVARKMKQKGLTNKDISDFTGLTVEEIDDL"
     gene            complement(14681..>15205)
                     /locus_tag="PK198_03190"
     CDS             complement(14681..>15205)
                     /locus_tag="PK198_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF37771.1"
                     /translation="EGEFSFLAFAPTPGDTLYQGKLYARELYATPLPAELVVLSACET
                     GIGEFKRGEGLVSLAYGFAQAGARSVVTSLWRVSDRETSDLMTFFYKNLQKGMAKDEA
                     LRQAKLEYMQGQSGWRTHPFFWAAFVPEGDMSPLNLPRPVPAWAWAVLGLLPAAGWWL
                     WRRRRGKIQAAASK"
     assembly_gap    15206..15294
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15295..16633)
                     /locus_tag="PK198_03195"
     CDS             complement(<15295..16633)
                     /locus_tag="PK198_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF37772.1"
                     /translation="MLKNAIESAFSITACLTQLKKPEEADVLLKELIKKIQYDPSSAN
                     PYERVKLAKHTLLKLIYFDAKNQYYWFLEDGDERKLKEACRRFENAVRVLEEIHSEHE
                     EAGSKQYILNRYHYVFEMSLNALFELYQCTGSEEVLAQAFGYTERSRAIFLSETLKRF
                     DAGTSSAIPDSLRKAIQQGQRKLAEVENRRYILSQKKLANDAPAMLDIHQELFEVTEN
                     LRLLEAEAKKLNPEFGRDKEITWIKPKEVQKRLKPGEGWLSYFAGDDDMYAFLITTEG
                     ASFFHIPKDFPLEKWVQEMRNAIYDYPTERSDSLLSVYQNRAWELYEKLLAPFAWRLP
                     QRLTIGADGLLKYLPFEALFTAPAPNCESSDCPYMLWRHSISYAPSATLWAADRKRSG
                     ARLEKKILAFAPEFQGEGNALAARDDRRSSLGPLLYNAEETKRISKMFRTKLIT"
     assembly_gap    16748..16984
                     /estimated_length=237
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16985..17445)
                     /locus_tag="PK198_03200"
     CDS             complement(<16985..17445)
                     /locus_tag="PK198_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37773.1"
                     /translation="MLQPVDTAALFKKEQWKLHYWRIAGNCLTQLNKNEEARKIYEQV
                     AAADWNRPEFQDTAAIELLLKATVFFISLPDAQKSMLCLSAGEKMSKKVHGPSSVDWV
                     RFQMQRANYKFFIESDFEGSVLLGRNALSVWLKKHGQWNKEARGLYQYLIN"
     gene            complement(17807..19795)
                     /gene="rpsA"
                     /locus_tag="PK198_03205"
     CDS             complement(17807..19795)
                     /gene="rpsA"
                     /locus_tag="PK198_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRF37774.1"
                     /translation="MLDNEKELHDEELPETPETIDSEDNSEEPAVTAEEEALPAAPDE
                     EDEIEEEDHEDEEPLELHLEQLISHDTAHDDFNWGIVNKHGQAYTKEEFAGFLEQYDS
                     TLSSVLENEVVAGRVTAINDGDVVLDIGYKSDGLIPLSEFRDMPGIQIGDKVEVYVEQ
                     QEDARGRLTLSRRKAKLLRAWERIVDSYTNGTIITGVVISKTKGGLIADCGGLETFLP
                     GSQIDIKPIIDYDIYVGKTMEFKVVKINETIKNAVVSHKALIESDLAEQRQAIIAGLE
                     RGQVLEGIVKNITDFGAFLDLGGVDGLLYITDISWGRINHPSEVLQLNQKVNVVVLDF
                     DENKKRISLGLKQLLPHPWEVLDAAVTEGSVVKGRVVNIEDYGAFLEIQPGVEGLVHV
                     SEVSWSNQPVNAREYFRLGEDCEARVVTIDRDDRKMSLSIKQLTQDPWSEIADRFPVN
                     STHQGEVKNLTPYGVFVELAPGIGGMVHISDLSWTKRYAHPSEFTKVGEKINIMVLDI
                     DRDNRKLSLGHKQTEENPWDTFENVFPVGSYHEATVLRRDDRGAIVQLPYGLEAFAPL
                     KHMRKDDGTLCDPEEVLMVKVIEFNRDDKRILVSHSRYLEDIRREADETVKKERTVEA
                     EQSRAAVKKVQASVEKATLGDMDIFSQIKEQLGDDLTQ"
     gene            19967..20893
                     /locus_tag="PK198_03210"
     CDS             19967..20893
                     /locus_tag="PK198_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF37775.1"
                     /translation="MDIRIQHLTKRYGAQTAVNNIDFEVKTGEILGFLGPNGAGKTTT
                     MKMIAGYLAPDEGDIQIGGQSILEHDMRRHIGYLPENNPLYLDMPVMDYLEYCAALHG
                     VPRAETPDRIRKMVALCGLNVEKHKRIGELSKGYRQRTGLAQAMIHDPEILILDEPTT
                     GLDPNQIVEIRKLIRDIGKEKTVILSTHILPEVEATCDRILIINRGNIAADGTADMLR
                     KQAQGRQVLHVRIEGTSSSEEMLRALQPLPGVEAVEMLDAGAGRFELQCLPDAQTAHE
                     VFQICVRRQWILTELVPFETKLEDVFRNLTMN"
     gene            20893..21798
                     /locus_tag="PK198_03215"
     CDS             20893..21798
                     /locus_tag="PK198_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF37776.1"
                     /translation="MSLRTRIAPTPSGFLHPGNGASFVLAWVVARATKGRVLLRIDDL
                     DRARFRMEYLEDIFFTLDWLGLDYDEGPASPQDFLDRFSQSLRTDTYHEALRQLAEQG
                     VLYACDCSRRHIREQSVGSLYSGFCRQRKLYLDTPHTAWRIRLPDDCEVAFSEWKQDV
                     PVSLNLPACMGDFVLRQKDGSPAYQIASLCDDLLWHINFIVRGQDLLPSTGAQMFLAQ
                     QLGYEDFQKATFFHHALLTDDSGEKLSKSAGSTALKSWREAGKSPDDVWRQAAQWLGL
                     EYREETPMEMAAMLSSGAKINAHFL"
     gene            complement(21905..>23230)
                     /locus_tag="PK198_03220"
     CDS             complement(21905..>23230)
                     /locus_tag="PK198_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF37777.1"
                     /translation="EYQLAGFFFSTKYLPLMLDRSTPPPIADISQLQLPPYRLIRLSN
                     GIPLYVVDMGTQDVVKLEVIFHAGRPFEHKKLVARATLSQLKEGTLHRDGAELAELLD
                     FYGATLQTPYSLDSSNIALYSLNKHFDKVLPLLSEMLECPVFPQKELESFIQRNQVDL
                     QIELTKPDVVAYRHITELIFGDAHPYGYNSYPETYSALSREDMIRHFQEHFTAGNCSI
                     FLSGKIQPGMTEMVDSHLGRVIPAGTSQPHLPPLPDVPPQRIHLPHPDKVQSAIRIGR
                     RLFNRNHPDYAGLYVLNTILGGYFGSRLMTNIREEKGYTYNIYSSLDPMRYDGAFFIG
                     TETGTEFVKDTITQIYNEIEILQQELVDDEEMEMVRNYLLGGFLNMLDGPFNVADIIK
                     TVVVEDLPEDYFQTVVHTVKTITPEDLQQLAQRYLRPEDLWEVSVGSGQ"
BASE COUNT         5269 a         5836 c         5968 g         5398 t
ORIGIN      
        1 caaaaaagga gcttgaacac cactgcggaa tcggcataat cgagcaatcg aacgaatggc
       61 ttcttgtcac ttttaaccgc cttatgctcc tgtgccgcct gtcgaagcaa ttccatcacc
      121 agatcaatgt ccgaactgta gtccacgccc acattgatgt caaaacgaga ggttacatgt
      181 tcgtgcgtcc agttgctgag atcattgccg gtaatgcgcg aattgggtaa gatgatgtag
      241 gtgtcgtcgc gggtcaatac cgtggtggtg cggagttcaa tttccatcac cttgcccacg
      301 atgccattca cctcgatgac gtcgcccact ttcactgtgc tgtccaacag aatgatgatg
      361 ccggaaatga agtcattaaa caaattctgt acccccagtc ccaagcccac caacaaggct
      421 gccgagccgg ccacaaatac cgtcacattg acgcccacaa aatgcaaacc caccgaaatg
      481 gctattacga taatcacata cttgaggact tgatagaatg catatttttt gccctcactg
      541 aagcgggtgg agcggtaaat ggatttgcgc aagacccaca gaaggagaga tgtaatcgct
      601 atgatgactg ctacgccgat gaggtgataa accgacaacg agaagctgcc gatgctcaga
      661 attttgtaat tgagaaactg ttccatacgg gttttatcgt ttggttttaa gtgccaacca
      721 aagtgttcct gctacgcctg ccgtaaaaat gagcactgca atcagttcgg cctgcgtgaa
      781 atgaaagccc agtgcatcgt agcgctcatt gacgcggatt ttttctatta aaaaacgctc
      841 tgtgccattg aaaatcaagt agataaaaaa caacatggcc ggaaccttta cccgcttgcg
      901 caatgcccac aaaatacccc cgatgccgaa agccatggcc gcctcataaa tcgaagtggg
      961 aaaaaccggc tcggccagcc tccggcaata acgcccgtcg catacgtcca tcgccacccc
     1021 gctgttgatg atgttgcgcg gatagtccat gctccacagc cagtcgggta ggaaccacca
     1081 gccgggttgc gtcccggccg caatgcccca gtcgccgtcg ccggaaaaat ggcagcccaa
     1141 acgccccacg ccatacgcca caatgagggc cggcgctacg gcatccataa cgtgcgccac
     1201 gggaatctgt tttttgcgca aatacagcca cacggccaca aaaccgaaaa tgagaccgcc
     1261 gtaaatggtg aggcccgcgc cggagaaaag tgtacctgcg gggtcgttga aaaactgctg
     1321 aggttcttcc aaaatggaga agactttagc gcccgccaca cccgaaacgg cagcaataat
     1381 ggtgatttcg ccgatgcggt cgtgcggaaa cacctcggca ctgcgggtga cggcnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccctcggtca gcgtttcggt gtggggaagg
     1681 agcagtccct cgcggctttt gcggcgcaat tcggcctgca atatcacggc ggcgctcagc
     1741 acggccaata ccaaaaacag gccgtaggtt ttgaaaatgg acagccagtt gtcggccggt
     1801 gtgccgatga ggtcgtgaaa aacgtaggag agatcagggt acatagtgaa tgtgttattg
     1861 attcccctcc tcaatgccac catcttccat cggcggtatt ttcgcctgcg cccattcctc
     1921 cgcatccggt gcagccttca atgactgata aacaacgctt tgcatgcgct gccggaaacg
     1981 ctgggaagac agtttggaat ttttttggga gggataagtc cgattgctca acaggatgaa
     2041 aatggtgccc gtttcgggat cggcccaggc gctggtaccc gtaaaaccgt cgtgcccaaa
     2101 tgcctcgttc gacatgcggg tggtcacatt ctggtgcatg cgggcgtcca tttgccgcat
     2161 gtcaaagccc atgccgcgcc gggtactggc cgggtgccgc gccgcaaagg tttttaccgt
     2221 agccggatcg aggtagcgtt ggccgccgta ataaccgtag ttgagcagca tttgaagcac
     2281 cgctgccagg tcctcggcat tggcaaacaa acctgcatga ccgctcacgc cgcccagcat
     2341 ggccgcgccc atatcatgca catagccttg tattttttgc attctgaagt atgcgtcgta
     2401 ttctgtgggc gctatgcggc tcagttctac gcgctgccag ggattgtagc cgatgctttc
     2461 caagcccatg gggcaaaaga aattgtcttc cagataggca tccaaagttt tcccgctgag
     2521 gcgatgcacc atgcgcgcca cgatgtaaaa acccaggtcg ctgtaccggt aaccacgcgt
     2581 aggcaacagt ggcgacgcaa tgatttcctg ccagaccgaa tcggcatagt cgcggcgaag
     2641 gtagaggttg gaggccaccg gaatatcgta ttcgtctatc gcgtccgggc agtaataatg
     2701 gtcttttacg accacttcct ccggcgtttt ttcaacagtt ttgcgataaa acggcagcca
     2761 gggttgcagc ccggcccggt gtgcgaggat gtctctgagc aggagatcgg ctttgttgga
     2821 accggctaat tcgggcaggt atttcacgat gggcgtttcg aggtcaacga ggccgtcgtc
     2881 gtacaatttc atcagagcca gggtgcctcc cgcaattttg gtcacggagg ccagatcata
     2941 cacatcatcc gcttgggtgg cctgtgtttt actgtatgtg tggtgtccga aggctttgtg
     3001 ataaacgatt tggccgtctt tggccgccaa caccacgcag cccggcgtag cgcctactgc
     3061 aatgtcctcc tgcgccagcc ggtccatttt ttcaaacgca gccgtgctca tgcccacgcg
     3121 ttcgggcagg ttatagccca ttttgaaaac aggcgaggta gtgacgccgg tattgaattt
     3181 gctcagcgga gaagccgtga ccggcaggcg tccgctgata ccgatggcgc cgaataaagc
     3241 ctgtgcggca aggtcttgcg caatcggatc atcctcgtaa gccacgagta cggggtcaat
     3301 ttgatcaaaa tggcgaaggc tgtaaggcgt gccgaacacc accagtacca cctgcgtgcg
     3361 gcgcgcgagg gtttccagaa aatccagtgc gctcttgctg aggccatagc cctgcgaggc
     3421 gaaagaactc atgccgtgca ggctcacgat gaccaattct tttttgccca gcgtttgcag
     3481 caggcggctg cgctcggagg aggagatttc cttcgggctt tggaacagcg gcattttttt
     3541 gtagctctcc aatcgcgcct gaaacttgga cttggccgat gcgccgatgc tgagggcggc
     3601 catatcggtg ctcatttttt tgatgggaag gaggttgccc ttgttgcgcg ccagcgtgag
     3661 actgtgctcg tacaatttcc atttcagggc gatggcgtcg ggcgagttga gttgctggtc
     3721 gaggctgtcg gtagaaatgg gggcaaaggt ggtaagaccc agccggtatt tggcccgcag
     3781 cacgcgcttc acatgctcgt tcacctcggc ctgcgcaatg cgcccttcgg ctatatagcg
     3841 tttgatttcg cggaaagcgg cctccatact ttcgggcagc accagcatgt cattgccggc
     3901 cagcagggcc tcggcctcta cttcgccggg tttgaaatgt ttcgtcacgc ctttcatttc
     3961 cagcgcatcg gtaaaggcaa gcccttcaaa gcccatttct ttgcgcagca gctcggtgac
     4021 ggtgtagcgc gagagggtgg tggggcggtt catgccggta tccaatgcag gcacattcag
     4081 gtgcgcaatc ataaagctgc ccacgccctg acgggccaac tcgcgaaacg gaaacagttc
     4141 gatgctgtcc agtctggctc tgtcgtgcgt tatggagggc aggtctaaat gcgagtccac
     4201 atcggtatcg ccgtggccgg ggaagtgttt ggcgcaagcc atgacgccgt tgtcctgcat
     4261 gcctttggcg tacatattgc ttttgacggc tacattatag cggtcttcgc cgaaggagcg
     4321 ggtgttgatg accgggttgg cggcgttgtt gtttacgtcc gccaccggtg caaaattgac
     4381 atggatgccg atgcgacggc actgccgggc cacttccttg ccgaactcgt aaatgagcca
     4441 gttgtcctga atggcgccga gcggcaactg cctcggataa ctgatggtgt tttctttaag
     4501 gcgcatgccc aggccccatt cgccgtcaat ggctaccatg agcgggatgt ggcgggtgag
     4561 cgcctggtat cggttggtga gttctacctg cttgacaggt gtgccctgaa aaaaacaaag
     4621 cgcacctaca tggtactccc ggatgagttt ttccacgccg gctatgtgat cggcgccttt
     4681 gtcggaatga gcgcgaatca taaaaagctg accgaggcgc tcatctggcg tcagaacgtt
     4741 gaacatagag tccacccagc ggtcttcatc agggttttga ccggccttca gcccggtgga
     4801 cgggtattgc ggcaagccgg cagccatcaa gaatccggct actgcggtga tggctaaaat
     4861 aagattcagg cggcgcggcc tccatgtctg gttcaattgc gtctgcattt cttttacaag
     4921 attgatttgg tgtagtgttt cgtcgtttcg cccggccaat ttagtataag tttttacttt
     4981 ttcgctcaac attggggcat attagtatgt aaagatattt ttgcgctccg aaatcaggaa
     5041 caatcatgct gacttatata ctcttcgcgt tgggttttgt ggctttggtt aaaggcgccg
     5101 actggttggt gacaggctct tctgctattg caaaacgttt gggcataagt gactttgtca
     5161 tcggcctcac catcgtgtcg gtcggcacct cggctccgga actgattgtc aacgtattgg
     5221 ccagcattaa cggcaatgca ggtattgcca ttggtaatgt gctgggcagt aatattgcca
     5281 atgtgttgct cattttgggc atctccgcca ttatttacgg cctgcccata cagcgcaata
     5341 cggtgctctc ggaaatcccg ttttcgctgg ccgctgcttt gttgatcggc tttttggcca
     5401 ataccgatat cttcgggctg gaacacaacg aacacggact gggccgcata gacggcgcca
     5461 tcattctgtt tttcttcggc ttgtttttaa tgtatgtatt cacgctccca tcggccaagg
     5521 aagacagccc ggaccaggcc attcaggcca tgtcgggcgc caaagcgggg ctgctcacgg
     5581 taatcggcat tgtggccctg tttttgggtg gcaaatgggt ggtggacggc gccgtgcaat
     5641 tggcccagtt gttcggcatg agcgaggctt tcatcggcct tacggtagtc gccatcggca
     5701 cctcgctgcc cgaattggtc acttcggcca tggcggcata ccggcgcaat accgacattg
     5761 ccgtgggcaa tgtggtgggt tccaacatct tcaacctcct gttgattttg gggctgagca
     5821 gcgtcattcg cccgcttcct tttcagcaaa gctcgaatat agaccttttg gtggtggtgc
     5881 tctccggcgc gctcatcatt ctggccttgg cgctgggccg ccgcttggtc atcaaacgct
     5941 acgacggggt catttttgtg ctgtgctatg tggggtatat tgcgtatttg ttgcagcggg
     6001 gctgaggttg ctttaccccg cagcccgcag caccacatta tctatcagcc tcacatcgcc
     6061 ggcccaggct gccgtgcagg ccaccacaaa atcggcttgg cggggatttc caacaggcag
     6121 gagggttcgc ccgtccacaa tttcaaagta ttcggggcgc agaccggcgc tttccatttt
     6181 ttgcatggcg cgcgctgtaa tggcagcggc gtccatttcc gggtaaagcc gtttggcgtc
     6241 caacaggcat tggtagatga cgggagcggc ggcgcggaat tcggggctga ggcgcacatt
     6301 gcgcgaactc atggctaagc cgtccgtttc gcgaaccgtt tctgcgacga tcagttgggt
     6361 gcttgaattc agttgtcgca gcatttccgc cacgatggcc gtttgttgaa aatccttttg
     6421 ccccatgtac aagcgctgcg gccgcaccat gtcgagcaat ctggccacta cctgcgctac
     6481 gcctttgaaa tgccccggac ggaaagcgcc ctccatcact gtttccaaat gcccgaagct
     6541 gaactcatag ctctgcccgc cggcgggata cacttcctct acggaaggca tcagcactgc
     6601 atggcagccc gctttggcca gtatggaaac gtctgcttcg ggtgtgcgcg gatattttcg
     6661 gaggtcttcc gggtcattga actgggtggg attcacaaaa atgctggccg ttgccaagtc
     6721 tccggctgct acggccctgc ggatcaaagc gaggtggccg gcgtgcagcg cgcccatggt
     6781 aggaacaaag ccaacagagg tgtgaggagc catgcttgtg tagcggctca aatcggcggc
     6841 atattgaaaa acgtacatac gctggcaagg tcggcgggcg tttgtaaacc gccgctcaaa
     6901 gatatacctt tttgttaagg ccgtattaag tgcgccggta attgattcaa agtcgttgat
     6961 tattactaaa tttgcgcgtt tattcagaaa cgcctctttt ggcggccatg taaagcatat
     7021 ttgaaaatcc gacaggcatt atgagcaaga aaaaagtgct gatcgtcacc caggagatgt
     7081 ctccttttac cgatccgtct gaattagcag atttcacccg caccctgccg caattcgttc
     7141 aggataaagg aatggaactc cgggttttaa tgcccaaatt cggcaccatc aacgaacggc
     7201 gccaccggct gcacgaagta gtgcgccttt caggcatgaa catcattgtg gacgacgacg
     7261 attacccgct gattatcaag gtggcgtcta tgccggaagc gcgcattcag gtttattttt
     7321 tagacaacga cgacttcttc cggcgcaagc acgtttttca ggatgaggaa ggccagattt
     7381 acgaagacaa tgtggaccgc atggtgtttt tctgtaaggg cgttctggaa acggttcgca
     7441 aattcggctg ggcgcccgac atcgtgcatt gccacggatg gatgacgagc ctcattcctg
     7501 catatctcaa gacggcttat aaaacagagc cgcttttcca gcactccaag atcgtgtact
     7561 ctttgtacaa tgacgaatta gaagaaaaga tgcctgaaaa tttcttcacc aaggcggcca
     7621 tcaacaacct ccatgccgaa gacctccacg cctatcaaaa cggcgccggc attaccctgc
     7681 acaaaggcgc tgcggcattt gccgacggcg tggtggtagg cagcccgact ttgagcgatg
     7741 tggcacaggc caccttagcc gacaccgata agcctgtact ggaattcagt gaagatgcag
     7801 ccctgccggg catcgtagag ttttacaact cgctgatgcc tgaggaagtc gaggctcagg
     7861 aatgattcat cttgcagcat gtttatacca acccgcagtc tgattcgggt agtctttatc
     7921 cgtgcgatcc atatcgggtc gtatcagttt ctaattcatt gatatttaca tgaaaaagcc
     7981 tattctacta ttgtggctcg cgttgggcgg cctgtttatt gccggctcct attccggctg
     8041 taccgacctc accgacctcg gcgccgattt gctggacgaa gaccgcctcg gattggatta
     8101 tgtggatacc gtttctatca ttgccaccac cgttacggtg gattccgtac tgacccaatc
     8161 cggcacctcg ctggccaatc aattggaccg ctttatgttc ggcgactaca atgacccggt
     8221 catgggccga gttactgccg gcgtttatgt acagcccctt ttagacaggg atcgcctcag
     8281 tctttttgcc attccgcgtc cgccttctta caaagacgct attttggact ccatcgtgtt
     8341 ggtactcacc ttggacagcg ccggatttta cggcaaaccg tctcagccgt ttgggctgga
     8401 ggtgctgcgc gtgattgaag acatgaacgc ctccgccaat tactactcta atgtcaattt
     8461 tgccacctcg gcagtaccct taggcagcgc acagttttcg cctaccattg acagcatcac
     8521 ggtattggat tataccagtc tgggaattga caccctccgg tacccgcacc ttcgcatccc
     8581 gctgaacatg gcgctggggc aggaattgat tgggctggac tccttaaatt atttgagcga
     8641 taccacattc ttaaacattt tcaaagggct ttacctgcgc cccacacagc aatccgacgg
     8701 cttggtagct tttaactgga gcagccttcg accgggcatt tttctctact acaccaatgc
     8761 gtcgggcttg ccgcgccagt accagtacga aatcaatgaa ttcagttccc gaacctcccg
     8821 catccggcat gactacgccg gctcgccggt agcgcctttc ctgaacgacc cggaacgggg
     8881 caagaacctg ctttttgcgc aggcaatggc aggaaccaat atcaaaatcg aaataccgca
     8941 tgcggcatcc cttcggaatg ccatcatcaa ccatgcagaa ctggaaattc ccgtcatcgc
     9001 tttttccggt gatgattcac tgaaatatgt caacaacccg gatgtatatc tcaccgtacc
     9061 tgatactaca ttggcgccca atcctatttc cgattttacc tttgcaggca gcaaccggat
     9121 cccgattttt gggggcgttt tcatcagggg gacagacgga aaaccagacc gttacaggat
     9181 gaatgtgtcc tcccaactca gtaatatggc ccaaggccgg gtagggaatg tgatgtatct
     9241 tcaaatcatt aataaggccc agcgtggcgg tcgtattgtc tttgccgggc ctgaaaacac
     9301 ccaacacaaa atgcgcctca aagtggcgtt caccaaacgt tgacccatgt gtggaattgt
     9361 aggctacatt ggaagccgtg aagcttggcc catcattatc aaagggcttc agagactcga
     9421 ataccgaggc tatgacagcg caggcgccgc cctgttgcac aacggagacc tcaaggtcat
     9481 caagaaaaaa ggcaaagtag ccgacctcac tgccgccgct gcctcttggg atgtgacggc
     9541 tcaacaaggc atcggccata cgcgctgggc tacgcatggt ccgccggacg acatcaatgc
     9601 gcacccgcac acctccggca acaaacgcct agcattggtg cacaacggca tcatagaaaa
     9661 ctatgccctt cttaaaaaag cacttcagaa agaggggcat gtatttgaag ggcaaaccga
     9721 tacagaggtg ttggtacacn nnnnnnnnnn nnnnngcaag tgcgtgaaac tctccccatt
     9781 gaagaagccg tcagaattgc cctcacccgc gttaccggcg cttatgccat tgtagtgatg
     9841 gaccgcaacg atccgggcaa gatcgtcgct gcgcgtaaag ccagcccatt ggtcgtgggt
     9901 atcggggagg atgagttttt tctggcctcc gatgccacgc ccattgtgga gcacaccaag
     9961 cgggtcattt acctcaacga cgaggaaatt gccattgtgc gccttggcca cgaaatcgaa
    10021 atccgcacca tagacaaccg gatgcaaacg cctttcattc aagagttgga tttagaaata
    10081 gagcaactcg aaaaaggcgg ttacgagcat tttatgctca aagagatttt tgagcaaccc
    10141 gaaaccattg ccgacgccat gcgggggcga ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn tatgcctctg aattgagata ccgcaacccg attctcggcg
    10381 aggacgatgt ggtgatcgcc atatcacaat cgggcgaaac cgccgatacc ttagctgccg
    10441 tagaactggc ccgcgaaaaa ggggctttgg tgtatggtat tgtcaatgtg gtaggctcat
    10501 ctattgcgcg gctcacacat tgcggctcat acatacacgc cggccctgaa ataggcgtgg
    10561 cttctaccaa ggcgttcact ggtcaggtca ccctgttgtc gctgatggcc ttggtgctgg
    10621 cgcacaagcg aggcaccatt tccgagtcgt atttcaggcg gctgctgata gaattggcgg
    10681 agattccgca aaaagtatcg caggttttag agtcggttca ggcnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnna attgaataca ttgcaggcga aatcaaagat cgcagcaatg ccatctatct
    10801 gggcaggggt tacaattttc cggtagcgct ggaaggggca ctcaagctca aggagatttc
    10861 ctacattcat gctgaaggct acccggcggc agagatgaag catgggccga ttgcgctgat
    10921 tgacgaacac atgcctgtga tcgttattgc caccaataaa agcgcttatg acaaaatcgt
    10981 gagcaatgta caggaggtga ttgcccgcaa gggtatggtg atagcagtgg tggaggaagg
    11041 agatcagatt atcagtaaga tggcggatta tgtaatagag atacctgcca cagaggagcc
    11101 ttttactccg ctgttgtcgg ttgttccctt gcagttgttg tcttatcaca ttgccgtgat
    11161 gcgcggctgc gatgtggatc agccgaggaa tctggccaag tcggttaccg ttgagtagtt
    11221 ttatttttaa aaaaacaatc cattgacaat gagaaaaatg gacatagagt acaatacctc
    11281 ccggaatgaa ttggtcatgc cggaatatgg tcgtcatgta caattaatgg tggaagaagc
    11341 caaaagactg accgacaatg cacatcgtca ggcttttgtg gaaaagatag tggatctgat
    11401 gtacattatg aatccacaac aacgcgaagt ggaagactac cgcgaccgtc tgtggaaaca
    11461 tgtgttccgc attgccgggt acgatctgga gggcgttatg ccgcccaacg gcattattcc
    11521 tactccggaa aacgacataa aaaggcccga ccacatcgaa tatccgggta cacaactgcg
    11581 cttccggcac tacgggcact acattcagga gcttatcaaa aaagccaaac aaatggaaga
    11641 cggccccatt aaggagggct tggtggcgtc cattggctcc tatatgaaat tggcctataa
    11701 aacctggaac aaagaccact atgtcagcga tgatgtgatc aaaaatgact tggtcaccct
    11761 gtccggaggc atcttgtcta tgcctgacga aatggccatt gaaaacctga cgcctatggg
    11821 gccgcctaaa aaaagcagcc ctcaaaacca gcgcggccaa caacagcagg gcggaggcaa
    11881 gtccaataag aagaaaaaag tctggaaaag gaaataatca tctgatatga ccttagatgc
    11941 ttttgaagta cacggcgggc agccattgtc gggcgaactt catccgcagg gcgctaaaaa
    12001 tgaggcgctg caagtgattt gcgccgtttt gctcagcgcc gaaaaagtga ctatttccaa
    12061 catccccgcc atacgcgatg tcgttttgct catcgaacta ttgcaggggc tgggagtaaa
    12121 aacaacgcaa attgcgccgg atacctatac ctttcaggca gatgaggtca acattgacta
    12181 catctattcc gaggattatt acaaaaaagg cggtcgtatt cgaggctcgg tgatgctgtt
    12241 ggggcctctg ttggctcgct tcggcaaggc gctgctgccc aaacccnnnn nnnnnnggcg
    12301 gcgacaaaat cgggcgccgg cgattggata cccacttgct tggattggaa accctcggcg
    12361 ctcatttcga ctatgacccc gaccgcagcc tttatcgcat agatgcgaca cagatgcgcg
    12421 gcgcctgcct gctgatggat gaaatttccg tcaccggcac agccaatgtg gtcatggccg
    12481 ccgtgttgac gcccggcgta acgacgattt acaatgctgc ctgcgagcct tatgtacagc
    12541 aattgtgcaa aatgctcaac cagatgggcg cccatatttc cggggtcggg tccaatttac
    12601 tgcacatcga aggggtggaa agcctgcatg gcgcggagca tcgcctcttg cccgatatga
    12661 tcgaggtggg tagttttatc ggcctggcgg aaattaccat caaaaatgcg cagatacagg
    12721 aactcggcat catcccgcgc atcttcgaac gcatgggcat tcagatggaa tttcggggcg
    12781 acgacatcta tgtgccggca caacaagagt atgaaattca gtcttttata gacggctcta
    12841 tcctcacggt gtatgatgcg ccctggcccg gtttcacgcc cgacctgatg agcatcgtgc
    12901 tggtcaccgc catacaggcc aagggcagcg tattggtgca tcaaaaaatg tttgagagcc
    12961 gcctgttttt tgtggacaaa ctcatagaca tgggcgctca gatcatcctc tgcgacccgc
    13021 accgagccac cgtcatcggc ctcaaccggc gcttccctct gcgcggcatc gaaatgacct
    13081 cgcccgatat tcgcgcgggt gtggccctgc tcattgccgc cctttcggcc aaaggcaaaa
    13141 gcaccattta caacatcaac cagatagacc ggggctacca gcaaattgac gagcgcctgc
    13201 gggctatcgg cgcccaaatc aaaagagtat gatcatttac aacgttaccg taaaaataga
    13261 acacgacctc cacgaagact ggctgaactg gatgcgccac acacacatcc ccgatgtgct
    13321 ggccaccggc ctgttcacag gccaccgcat cagcaaagtc ctgggtgacg atgatgcgca
    13381 cggcgtcact tatgccatcc agtacgaatg ccccgatctg gacacttttc tccaatacca
    13441 gcaacaacac gccgctcgcc tccaggccga tcacgcccgc cgctaccagg ataagtatgt
    13501 ggcgttcagg actttgctgg aggtggtggg gtagtgcccg atattgggca ggacgatcaa
    13561 gccgagcgcc gacacggtga cgtgggtggc gccggggcct gttatcagtt tggtgcgaaa
    13621 cattttgctg atgcgcttgg cctcttccgc attgtacaac acgggcaaag tagcggctat
    13681 acctggttta cgaagcaatg cagatatagt aaaaaaaggc cgacccaatc agtaagctat
    13741 tcgcggcacg ttcctaaaca ggaggtctgc atggctaagt ccgttaggga aagttgacaa
    13801 atcacaaatt ttgaggtatt tttgtataaa aatttgacta tgcagtttgt agatatcaag
    13861 aatgacatcg cctttcgcaa gattttcggc aacgagaaca agaaaatcat cctcatctca
    13921 ttcctcaacg ccgtgatgaa gttgaaagga aaggattcca tcgaagatgt tgaaattctc
    13981 aacccctacc aactgcctat tatcaaaaat ctgaaagcgt ccatcattga cgtaaaggcg
    14041 agagacaaga aaggcaaaac ctacatcatc gaaatgcagg tagccgagcc agacggcttg
    14101 gacaaacggc tactttacta cgccggcaag gagtattccc aacaaatcga aagtggcgag
    14161 ttttacaccc aactcaaacc agtaattttc atcggaattt ttgacttcaa gtttacggaa
    14221 ggtgacaaat acctttcgca tcatgccgtt tgcgatgttg agaatggcga gcgcatcatc
    14281 aaggacatgg acttctactt tgttgaactt cctaagttta aaaaatcgct gtctgaactt
    14341 caagaagtta ctgacaaatg gatatttttc ataaaagagg cagaaaatct tgaagttgtt
    14401 cccgaaaatg tggatgatga aggcttgaag gcggcctacc acgatgccaa taaacactcg
    14461 tggactaagg aagaattaga agcctatgac tacgcagcaa tgagggaaca agatgaaaga
    14521 ggcaaggtag cattggcaga aaagagggca gataaaaaag caaaaaatga agtcgcaaga
    14581 aaaatgaagc aaaaaggtct tacaaacaaa gacatctcgg attttacagg tttgactgtg
    14641 gaagaaatag acgacttgta ggggcgatca ggaaaaacct ttacttcgat gcggctgcct
    14701 gtattttgcc ccttctcctc ctccacagcc accagcccgc cgccggcaac aaacccaata
    14761 ccgcccaggc ccacgccggt accggcctcg gcaaattcaa aggcgacata tcgccctccg
    14821 gtacaaaggc cgcccagaaa aacgggtgtg tgcgccaacc gctctgcccc tgcatatatt
    14881 ccaattttgc ttgccgcaag gcctcgtctt tggccatgcc tttttggagg tttttataaa
    14941 aaaacgtcat cagatcggag gtctcccggt cgctcacccg ccacagcgaa gtgacgacgc
    15001 tgcgtgcgcc ggcctgcgca aatccataag ccaaactcac caaaccctcg ccccgcttga
    15061 actccccaat gccggtttcg caggcgctga gcaccaccaa ctcggcaggc agcggcgtgg
    15121 cgtacagctc gcgggcgtac agcttgcctt gatacaaagt gtctccgggc gtaggggcaa
    15181 atgccagaaa cgagaactcc ccttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgtta
    15301 tcagtttggt acgaaacatt ttgctgatgc gcttggtctc ttccgcattg tacaacagcg
    15361 gccccaggct gcttcttctg tcatcgcgtg ctgcaagggc attcccctcg ccctgaaatt
    15421 cgggcgcgaa ggccagtatt ttcttttcta atcgggcgcc gctccgcttg cggtcggcag
    15481 cccacaaggt ggccgagggc gcatagctga tgctgtgcct ccagagcata tatgggcaat
    15541 ccgacgactc gcaattgggg gcgggagccg taaacagcgc ctcaaatggc aggtatttca
    15601 gcagtccgtc tgcacctatg gtcaatcgct gcggcaaacg ccaggcaaaa ggagccagca
    15661 gcttttcgta caactcccag gcccggtttt gataaacgga caacagacta tcgcttcttt
    15721 ccgtggggta gtcgtatatg gcattgcgca tttcctgcac ccatttttcc aacggaaagt
    15781 ctttgggaat atgaaaaaac gatgcgccct ctgtggtaat caggaaagcg tacatatcat
    15841 catcgcctgc aaaatagctc agccatcctt ctcccggttt caggcgcttt tgtacctcct
    15901 tcggtttgat ccaggtgatc tctttatccc ggccgaattc ggggttcagt ttcttggcct
    15961 ctgcctccaa taaccggagg ttttccgtta cctcgaataa ttcctgatgg atgtccaaca
    16021 tagccggcgc gtcgtttgcc aactttttct gcgacaatat atagcgcctg ttttctacct
    16081 ctgccaattt gcgctgcccc tgctgtatgg ccttgcgcaa gctgtcgggg atggccgagg
    16141 acgtcccggc atcgaatcgt ttgagcgttt ccgacaaaaa aatcgcccgg ctgcgctccg
    16201 tgtatccaaa tgcttgtgcc aatacctcct ccgagccggt acactgatac agttcaaaca
    16261 gcgcattcaa agacatttca aatacataat ggtagcgatt caatatgtac tgcttagatc
    16321 cggcttcctc gtgttcggag tgtatttctt ccagcacccg gaccgcgttt tcaaagcgtc
    16381 ggcaggcttc ctttagcttt cgctcatcgc cgtcctccaa aaaccagtaa tactgattct
    16441 tggcatcgaa gtagatgagt tttaacagtg tgtgttttgc caattttact cgttcgtatg
    16501 gattggccga cgatggatcg tattgtattt tttttataag ctctttgagc agtacgtctg
    16561 cttcttccgg tttttttaat tgagtcaggc aggcggtaat gctaaatgcg gactctatcg
    16621 catttttcag catctcattt tgcatggagg aataaatagc gtaggcattc ctaaagtgtc
    16681 gttctgcttc ggtgtatttt ttttgatgat acagggtcgt gccaattctt gagtaattaa
    16741 aagcgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnaagtta ataagatact gatatagccc gcgagcctct ttattccact gcccatgctt
    17041 tttcagccat acagaaagcg cattccttcc tagcaatacg gagccttcaa agtcgctctc
    17101 tataaaaaat ttataatttg cccgttgcat ttgaaaacgc acccagtcaa cggaggaagg
    17161 gccgtgtact ttttttgaca ttttttcacc ggcagacagg cacaacatac tcttttgagc
    17221 atcgggcagg gagataaaaa atacagtcgc tttcaaaagt aattcaatgg cggcagtgtc
    17281 ctgaaactcc ggcctgttcc aatctgcggc tgctacctgt tcataaattt ttcgagcctc
    17341 ttcgttttta ttcaattggg tcagacagtt tccggcaatt cgccaatagt gaagtttcca
    17401 ttgttctttt ttgaacagcg ctgcggtatc aacaggctgc aacattttcc aggcggcctc
    17461 gtattgttga gcctgataca attgtgtggc tttttccaac acggcgtgta cgcggtccga
    17521 ctgcgcccga gcatctggag gaaagacgag gaagtaaaaa attgtaaaaa gaaggagcgg
    17581 agtaggtttc atgcagtgat tagatttgga aagaaacaaa aaccgccata aaagtctaaa
    17641 aaattggaat acaaagaata atttttttat cacccggttg cgggcaggta tcatggaaaa
    17701 agaggctacc ccgataagtt cttcacaaca cattcctaaa aaaaaagagg cttcgacagt
    17761 tgtcgaagcc cctctttttt ataaaatagt cagtttatta tcaagactat tgagtgaggt
    17821 catccccaag ctgctccttt atctgcgaga agatgtccat atcgccgagg gtggcttttt
    17881 ccaccgaagc ctgcactttc ttcacagcgg cgcggctttg ttcagcttct acggtacgct
    17941 ctttcttgac ggtttcgtcg gcttcgcggc gaatgtcttc gaggtatcgg gagtgagaaa
    18001 cgaggatgcg cttgtcgtcg cggttgaact cgatcacttt caccatcagt acttcttccg
    18061 gatcacagag ggtgccgtcg tctttgcgca tgtgcttgag gggcgcgaaa gcctccaagc
    18121 catagggcaa ctgcacgatg gcgccgcggt cgtcgcgacg gagcacggta gcctcgtggt
    18181 aagaacccac cgggaacaca ttttcgaagg tatcccaggg gttttcttcg gtttgtttgt
    18241 ggcccagaga gagtttgcgg ttgtcgcggt caatatcgag caccatgatg ttgatcttct
    18301 cccccacttt cgtgaattcg gaggggtgag catagcgctt ggtccaggac aggtcagaga
    18361 tgtgaaccat gccgccgatg ccgggggcca attccacaaa tacgccgtag ggagtgaggt
    18421 ttttcacctc gccctgatgc gtgctgttga ccgggaaacg gtcggcgatt tcgctccagg
    18481 ggtcctgcgt gagctgcttg atggagagcg acattttgcg gtcgtcgcgg tctatggtaa
    18541 cgacgcgggc ttcgcagtct tcgccgaggc ggaaatattc gcgggcattg accggctggt
    18601 tgctccagga aacttccgaa acgtgaacca gaccttctac gccgggctga atttcgagga
    18661 atgcgccgta gtcttcgatg ttgactacgc ggcctttgac cacagagcct tccgtgacgg
    18721 cagcgtcgag tacttcccag gggtggggca acaattgctt gagacccaga gaaatgcgct
    18781 tcttgttctc atcgaaatcg agtaccacca cattgacctt ctggttgagt tgcagcactt
    18841 cgctcggatg gttgatgcgg ccccaggaaa tgtcggtgat gtaaagcagg ccgtccacac
    18901 cgccgaggtc gaggaatgcg ccgaaatcgg tgatgttttt cacgatgcct tcgagtacct
    18961 ggccgcgttc gaggccggca atgatggcct gacgctgttc ggcgaggtcg ctttcgatga
    19021 gggctttgtg agataccacg gcattcttga tggtttcatt gattttaacc accttgaatt
    19081 ccatcgtttt gcctacatat atatcgtagt caatgatcgg tttgatgtca atctgagagc
    19141 cgggaaggaa ggtttccaaa ccgccgcagt cggcaataag accgcctttg gttttgctga
    19201 tgacaacacc tgtgatgatg gtgccgttgg tgtaggagtc cacaatgcgt tcccatgcgc
    19261 ggaggagttt ggccttgcgg cgggaaagcg tgaggcggcc gcgggcatcc tcctgctgtt
    19321 ctacatagac ttccactttg tcgccgatct gaatgcccgg catatcgcgg aattcagaaa
    19381 ggggaatgag gccgtccgac ttgtagccaa tgtccaatac cacatcgccg tcgttgatgg
    19441 cagtcacacg accggcaacc acctcatttt ccaatacgga agagagtgtt gagtcgtatt
    19501 gctccaaaaa tccggcaaat tcttctttgg tgtaggcttg gccatgcttg ttgacgatgc
    19561 cccagttgaa gtcgtcgtga gcagtgtcat gcgaaatgag ttgttcgagg tgcagttcca
    19621 gaggctcttc gtcctcgtga tcttcctctt cgatctcatc ctcctcatcg ggagctgcgg
    19681 ggagggcttc ttcttcggct gttacggccg gttcttccga attgtcctcg gaatcaatgg
    19741 tttcgggagt ttcgggcaac tcctcgtcgt gcagttcttt ttcgttgtcg agcattgaaa
    19801 gaatgctttt aatggtgaaa aaataaggtt gattcaaaag tggccgcaaa ggtaggcata
    19861 agttttcatt ctgaacgact tagaaaaaat ctttttcgca gaacccggag attcttatca
    19921 tctgcctaac tttaccgccg tattgtgaat tattagaaac aagacaatgg atatccgcat
    19981 tcaacatctg accaagcgat acggcgctca aacggctgtg aacaacatag attttgaggt
    20041 gaaaaccggc gaaatcctgg gctttctggg gccaaacggc gccggcaaaa ccaccaccat
    20101 gaagatgatt gccggctatc tggcaccgga tgagggcgat attcaaatag gcggccaatc
    20161 tattctggaa cacgacatgc gacggcacat cggctacctg cctgaaaaca acccgcttta
    20221 tttggatatg cctgtgatgg actatctgga gtactgcgcg gctctgcatg gcgttcctcg
    20281 tgccgaaact cccgaccgca tcagaaaaat ggtggctttg tgtggcctca atgtagaaaa
    20341 acacaaacgc atcggagaac tttccaaagg ctatcgccaa cgcaccggac tagcgcaggc
    20401 catgattcac gatcctgaaa tcctcatttt ggacgaaccc accaccggcc tcgatcccaa
    20461 ccagattgta gaaatccgca agctcatccg cgatatcggc aaggaaaaaa ccgtgattct
    20521 cagcacgcat atcttgccgg aagtggaggc cacctgcgac cgcatcctca tcatcaaccg
    20581 gggcaatatt gccgccgacg gcaccgccga catgctgcgc aaacaggctc aaggccgaca
    20641 ggtgctgcat gtgcgcattg agggtacttc ttcgtccgag gagatgctgc gggccttgca
    20701 gcctttgccg ggcgtggaag ccgttgaaat gctggatgcc ggggccgggc gttttgaatt
    20761 gcaatgtctg cccgatgcac agaccgccca cgaggtgttt caaatctgcg tgcgccggca
    20821 atggatactg acagaattgg taccgtttga aaccaaattg gaggatgttt tccgcaacct
    20881 caccatgaat tgatgagcct gcgcacccgc atcgctccta cacccagcgg atttttgcat
    20941 cccggcaacg gcgcctcttt tgtgctcgcc tgggtagtag cacgggctac aaagggccga
    21001 gtgctcctgc gcatagacga tctggaccgt gcgcgcttcc gcatggagta tctggaggat
    21061 atttttttca ccctcgactg gctgggctta gactatgacg aaggcccggc ttccccgcag
    21121 gattttttag atcgcttttc gcaaagcctg cgaacggata cttaccacga ggcgctccgg
    21181 caattggcag aacaaggtgt tttatacgcc tgcgattgtt cgcgcagaca cattcgggag
    21241 cagtctgtcg gcagcctgta ctccggtttt tgccggcaga gaaaattgta cttggatacg
    21301 ccccacaccg cttggcgcat caggctgccc gatgattgcg aagtagcttt ttcagaatgg
    21361 aaacaggatg tgccggtatc gctgaatctc ccggcctgca tgggcgattt tgtactccga
    21421 caaaaagacg gcagcccggc ctatcagatc gcatccttgt gcgacgacct gctgtggcac
    21481 atcaatttca ttgtgcgggg ccaggatttg ttgccgtcca ccggcgcgca aatgtttctt
    21541 gctcaacagc ttggatatga agactttcaa aaggcgacgt tttttcatca cgccctgctg
    21601 accgatgatt cgggagaaaa actctccaaa tctgccggct cgacagcatt gaaatcctgg
    21661 cgggaggcag gcaaaagccc tgatgacgta tggcgacagg cggcccaatg gctgggtctg
    21721 gaatatcggg aggaaacgcc gatggaaatg gcggctatgc tgagtagtgg ggcaaaaata
    21781 aatgctcatt ttctttaggc caaaggatta gataaggcac ttaaaatgaa acgtttactt
    21841 atctaatcct ttggcgaaaa tgatcacttt atttttttat cgcccctgag ccaataagtt
    21901 gattttattg cccgcttccc accgatacct cccataggtc ttcgggccgg aggtagcgtt
    21961 gggcgagttg ttgcaggtct tccggggtga tggtttttac cgtatgcact acggtttgga
    22021 agtagtcttc cggcaggtct tccaccacca ccgtttttat aatgtcggct acgttgaagg
    22081 ggccgtcaag catgttgaga aacccaccga ggaggtaatt gcgcaccatt tccatttctt
    22141 catcgtccac cagctcctgt tgcagtattt ctatctcgtt gtaaatctgc gtgatggtgt
    22201 cttttacaaa ctccgttcct gtttcggtgc cgataaaaaa cgcgccgtcg tagcgcatcg
    22261 ggtccaatga ggagtatata ttataggtgt atcctttttc ctcgcggata ttggtcatca
    22321 gtctggagcc gaaatagccg cccagaatgg tattgagcac atacaaaccg gcgtagtcag
    22381 gatggtttct gttgaaaagg cggcgaccga tgcggatggc cgattgtacc ttgtcgggat
    22441 gaggcaggtg tatgcgctgc ggagggacat cgggcaaagg cggcaaatga ggctgcgatg
    22501 ttcccgcagg aattacccgc cccagatggc tgtccaccat ttccgtcatg cccggctgga
    22561 tttttccgct gagaaatatg gagcaattgc cggcggtaaa atgctcctga aaatgccgga
    22621 tcatatcttc ccgtgaaagc gcggagtacg tttccgggta actgttgtat ccataaggat
    22681 gcgcatcgcc gaagatgagt tcggtaatgt gccggtaagc cactacgtcg ggtttggtca
    22741 attcgatctg caagtccacc tgattgcgct gaatgaaaga ctccagctct ttctgaggaa
    22801 atacagggca ttccagcatt tccgagagca ggggcagcac cttgtcgaag tgtttgttga
    22861 gggagtacag cgctatgttg gaggagtcga ggctgtaagg cgtttgcagg gtggcgccgt
    22921 aaaaatcgag cagttcggcc aattcagcgc cgtctctgtg cagggtgcct tctttgagtt
    22981 ggctgagggt ggcgcgggcc acgagttttt tgtgttcaaa agggcggccc gcatggaata
    23041 tgacctccaa tttgactacg tcctgagtgc ccatgtccac cacatagagg gggatgccgt
    23101 tgctgagccg gatgaggcgg tagggcggca gttggagttg gctgatgtcg gcaataggcg
    23161 gcggcgtgga gcgatccagc ataagaggta ggtacttcgt tgagaaaaaa aagcccgcga
    23221 gttggtactc
//
LOCUS       DAORLF010000020        22541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1261_length_22541_cov_2.092769, whole genome shotgun sequence.
ACCESSION   DAORLF010000020 DAORLF010000000
VERSION     DAORLF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22541
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_22541_cov_2.092769"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..310
                     /locus_tag="PK198_03225"
                     /pseudo
     CDS             44..310
                     /locus_tag="PK198_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            complement(464..1552)
                     /gene="ddlA"
                     /locus_tag="PK198_03230"
     CDS             complement(464..1552)
                     /gene="ddlA"
                     /locus_tag="PK198_03230"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRF37778.1"
                     /translation="MKKKIAVVFGGKSAEHEVSLNSASNIFNAIDLHQFDAILLGVDP
                     QGRWQYNPEYPTHQVNLPQNDYFAQAQPVWLDNREGRAAIISKTNLEILDTFDAAFSI
                     IHGTNGEDGVMQGFFRSLDIPFVGPDVLGSAVCMDKDVTKRLLRDFHIPLADSITLFR
                     HEPLSLSFAQVVEKLGLPLFVKPSNAGSSVGVSKVTDEAGFHKALAEAFKFDHKILIE
                     EAVIGKEVECAVLGNEHPRASVLGEIIPVKDFYSYEAKYKDADGALLNIPADIDPAVS
                     DQIRQTAVQAFQIACCEGLARVDFFLRSDNSFVLNEINTLPGFTEISMYPKMWEATGL
                     AYSDLITQLVELGMARHERDGKLKTNRQ"
     gene            complement(1833..3116)
                     /locus_tag="PK198_03235"
     CDS             complement(1833..3116)
                     /locus_tag="PK198_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37779.1"
                     /translation="MQDKYTHHTSSSSPADLGWERMAALLDAEMPAQASAAPNSRKRR
                     GLIWLWLLAGFIITAGAAATIYQHQRLAQEQLPEKPAVEALKPVMAQVERPTETVISA
                     SESTSVAEMKTVSPPSWATLTLSPAAQQHTMAATMQTASEYIESAASQTFTEAAPAVE
                     STQVQQVALTAPSVKRWAGASPMLQSAVPAGLLNARPDLGLEPTIKTKRNARKKVRFG
                     VELATGIAAGSGSVGNFSAGAVAIWKPGTMISARSGLLAGSNTGDLFMSTAARQSLVN
                     DPNSGMLDNSTPLQALESRAFSGQTYRFQAVTLQLPAMLSYYLSPRLSVEGGVTAGLL
                     LNLQNVVESSSEMDQLTNTGSSPTANFSQAQISSAVSNSVARWELLASAGLRCRLTPK
                     FSVGLHYQHGLNDLLPDSSLQAMQRNVRLSGTMLF"
     gene            complement(3106..3666)
                     /locus_tag="PK198_03240"
     CDS             complement(3106..3666)
                     /locus_tag="PK198_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF37780.1"
                     /translation="MAEQKTYTEKELVEGCIRNDRRCQEALYRKHFDTMYRMVCRYSD
                     DRDLALEIVNNGFLRVFKKLDTFTFSGSLEGWIRRLVFHALSDHFRRQPKQVHFLDIE
                     DRDAPQNGNVLNDLYFEDIVKLVDMLPNSSREVFWMYAIEGYAHAEIAKRLNISEGTS
                     KWHLSMARQKLRELLQFHYNVNYHAG"
     gene            complement(3685..4674)
                     /locus_tag="PK198_03245"
     CDS             complement(3685..4674)
                     /locus_tag="PK198_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37781.1"
                     /translation="MRYIQLIVFLLCIPLAMQAQTPMQDVVYLKNGAVIKGVILDYRP
                     GGQLVIDIGNGREVTFQDSEVAKIQQEPFSNQGKTDGTVDFLFLYNGSIFKGEIVSET
                     PTQTILKLKNGEKVTLNAKEIKAVQRNQPADSKPTLESGSTAQQAYDYHYISMTKKPK
                     EKKEYAFRERGWFNRTSFTMPNGVYLGEAQFGAGLHNLTGFQFNRMLGAGIGLGFDTF
                     NPEGGENVASVYGEAQSFLTKTKTAPFVSFAAGYGFAFRNRNNFITKAEGGLRFHPAV
                     GLRLGADKDLNLMLDVGYIFQTATYTREFDFFDQTEVRKVDFRRFTLRLGAMF"
     assembly_gap    4731..4743
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4745..>5766)
                     /locus_tag="PK198_03250"
     CDS             complement(4745..>5766)
                     /locus_tag="PK198_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37782.1"
                     /translation="TPIAFINLPGNVDMAVRNNILYADNYVDLVSLDITTPSNPVYLS
                     RTESVFPNAFPFTQGLGYTVEYRETPTTRVVPCDQNMGIFFWERGGIMMDMASFSGVN
                     LQSSSGGASFAPSAVGIGGSMARFTIAATKYLYVVDEYNLRVFDLTQATAPTQAAVVS
                     VGWGIETIFPHGDNLFIGSNNGMFIYSIANPLQPAQLSRFQHAQACDPVFVSGDIAYV
                     TLRGGTVCQNFTNQLDVINISDLRNPRLIKSYAMTNPHGLSVVNDVLYICEGTEGLKV
                     FDASDVNKITQNLLDHEKGFNAFDVIVLPHNNVAMVIGEDGFHQFDASDPTNLRKLSV
                     LGINR"
     assembly_gap    5767..5833
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5834..6046)
                     /locus_tag="PK198_03255"
     CDS             complement(<5834..6046)
                     /locus_tag="PK198_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37783.1"
                     /translation="MKKGTIFSILSVALALSAMLTLGGCLQDKCVSITTYTLFEPVYK
                     LPEEMRQPIVTEAPRALENPGKIYYYQ"
     assembly_gap    6181..6203
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6351..6745)
                     /gene="ssrA"
                     /locus_tag="PK198_03260"
     tmRNA           complement(6351..6745)
                     /gene="ssrA"
                     /locus_tag="PK198_03260"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     assembly_gap    6839..6855
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6934..8142
                     /locus_tag="PK198_03265"
     CDS             6934..8142
                     /locus_tag="PK198_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF37784.1"
                     /translation="MKLGIIREGKVPPDSRTPLTPEQCAHLIRDNRLDVRVEPSPIRC
                     YPNAEYTDFHVPLSHDMHDCDVLMGIKEVPIDQLIPGKTYLFFSHTIKAQAYNRKLLQ
                     AILAKNIRLLDYEVMTDERGRRLIAFGKFAGMVGAHNALWTWGQRTGAFSLPRMKDCF
                     DYEAAKEHYHHIQWPSLKIVVTGSGRVGTGTIEVLHDMGILQVSPEEFLENEFDEAVF
                     TQITPYQYVARKDGEHFHTRDFYAHPDLYQSIFMPFAAASDIMINGIFWDKRAPAFFT
                     LDDMRRPDFRIRVIADVTCDIAPESSIPATVRPSSIANPVFGFDPVSEQEIPAFDSRG
                     IDVMAIDNLPSEMPRDASRAFGDMFISHILPELLLPGSKVVERATVAVDGQLGPHFQY
                     LQEYAGLVEV"
     gene            8291..>9086
                     /locus_tag="PK198_03270"
     CDS             8291..>9086
                     /locus_tag="PK198_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF37785.1"
                     /translation="MPYISHSTYHPPLPFRQGHINTVFAALGRRLPAMPYIRERWDTP
                     DGDFLDVDWAAQGNSRRVLLALHGMEGDTSRPYVRGILRAFAMHGWDALSFNFRGCSG
                     ESNRLPRAYHMGETGDLDFVLHELLQRHDYDEVALVGFSLGGNVLLKYLGEQGRALHP
                     AVKKAVAFSVPCHIPSSERAIARPINRPYVWRFLRTLNPKMQRKAALFPGLINAAPPL
                     PRSLREFDERFTAPLHGFAGAEDYWQRSSSLPWIEKITVPTLLVNAQ"
     assembly_gap    9087..9247
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9393..9956
                     /locus_tag="PK198_03275"
     CDS             9393..9956
                     /locus_tag="PK198_03275"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRF37786.1"
                     /translation="MAVKIRLQRKGRKQAPFYHIVVADSRSPRDGRFIEKIGTYNPLT
                     RPATIDIDRDAAFNWVMNGAQPTDTARAILRFKGVYYRKHLALGVAKGALTQEDADKR
                     YQAWVEAKESRIAARAAKTAEEKAAFYAMISGQPKPKAAPAPAVVEPEAEVAEEVAEA
                     AEEVVEAVVETAEEAAVEAAETDTEQA"
     gene            10059..11447
                     /locus_tag="PK198_03280"
     CDS             10059..11447
                     /locus_tag="PK198_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37787.1"
                     /translation="MYELNQKYIEQLELIAENIQDSEELAQYLEEEEEEFYNQLKEAY
                     EPRLAEMYQEVAHTNPLQLVSLELVLLDPAFEGLFLPRILGYSVLRGQINEHYKYVRP
                     QEHFKQILLTICDSSNFDLLKKRIGQTIQIGFALSSDIWITNLIAGITNKKVSNYLQA
                     QKLERFLDENERKNGYVRYARQFHNENYLSADFPTDRTSLLLNAAQLKRFLTYRISAK
                     VDNSNLINHIDDLVAHEDFYGVREHFELMLLYGCFFEHDEASEAILREVVNDVRRNHP
                     EVAQWGMEYVRELHRHSQLDLGPANEKRFAALLDKSINDELAEYFQLADTVHTKGYTN
                     IEAQDAVKEFYLQHEGLSVANECVRQMIFRYFRSFINNLENDAYTDFFEICRLFQVYM
                     NIFSNQQFNQDLEDLSMEYVGKLLLHYTDKRGKDYQDIKRFVSSTLQDFRFVTEKEVV
                     EMFKTKRKKKED"
     gene            complement(11499..>11919)
                     /locus_tag="PK198_03285"
     CDS             complement(11499..>11919)
                     /locus_tag="PK198_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRF37788.1"
                     /translation="LIHSVDSLDLLNEIDKQAAKNHRSIDCLLQFHIAQESSKYGLLP
                     DAPDSWSQNQFEAWPNVRICGVMGMATFTEDMEQVRGEFQLLRRIFGRLRERFFFENE
                     HFRHLSMGMSDDYPVAIETGSTMVRIGTLLFGDRAPA"
     assembly_gap    11920..11945
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11946..12171)
                     /locus_tag="PK198_03290"
     CDS             complement(<11946..12171)
                     /locus_tag="PK198_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRF37789.1"
                     /translation="MSNLYPSILNELRAKEVQLVAVSKTQPEQAILELYRQGQRDFGE
                     NRVQELLPKYEALPKDIRWHLIGHLQSNKVK"
     assembly_gap    12288..12309
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12310..12907
                     /gene="coaE"
                     /locus_tag="PK198_03295"
     CDS             <12310..12907
                     /gene="coaE"
                     /locus_tag="PK198_03295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRF37790.1"
                     /translation="IMLKAGITGGIGSGKSTVCRVFEWLGVPVYYADDRAKQLMTEDA
                     ALRSEIRILFGDEAYLPDGSLNRAHIAGIVFSDADMLRQLNALVHPAVHRDGAAWHLR
                     QTAPYTLREAALLYESGGYRLLDKMVAVTAPAELRIERVMARDGVDRSAVEARLNKQW
                     PEEKKVEMADFVIVNDGTTPLLPQILQVHRALSLEADK"
     gene            12912..13649
                     /locus_tag="PK198_03300"
     CDS             12912..13649
                     /locus_tag="PK198_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37791.1"
                     /translation="MSNTPEEARIEAEVQKRLAGIMAEQQQQWAAAMEAMMKNSVGGV
                     EQANTALAKEQKKLERELDRARELHRKAELEGEKMAMDAYEKHRAQYDESAKIKVLRD
                     LTSLHIEAGKTDEEIIAWIGVEQDFIENIRVVVQRVQKFRAENQAKRVIPSGRPVVRF
                     QDYGRSGTVYFESEEAKFDLWWEMGVHALIIVDAPTPDMWEDKTGLPPERRAEILNFI
                     GEEIVATQTISGGSFIVGENVLTIYVD"
     gene            13646..14221
                     /locus_tag="PK198_03305"
     CDS             13646..14221
                     /locus_tag="PK198_03305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF37792.1"
                     /translation="MILGASYLYPRNILFFIQKPPIIAATASYDFLIVGGGLFGVYAA
                     LYLSGHGCSVALVEMEDELLKKASIVNQARLHSGYHYPRSVATARMSDDHKERFIADH
                     RPFVLFDFEKYYAIDKYGSFTDAGQFERFCDFINIRRDKVEDTHLWFNFDRIEALYAT
                     TEYTFDPVLIADHYRQRIEKQKGVTIFKSTV"
     gene            14293..14856
                     /locus_tag="PK198_03310"
     CDS             14293..14856
                     /locus_tag="PK198_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37793.1"
                     /translation="MQAAQVINATYAGSNAVNRTFGVDDISLMHEISEIAFVTSPQIG
                     RIGLTVMDGQFGSIMPYGLSGLLSLSSVAYTHHRVSYDNLPTFHCQSQAPNCRPQQPG
                     NCNFCPAQPPSNAAKMMAQIKHYFRPEVELRYYFSLFTIKSKLKASYIDDGRPTEISC
                     LNEHPRYYCIFAGKINSIYEIEKIIVF"
     gene            14872..16137
                     /locus_tag="PK198_03315"
     CDS             14872..16137
                     /locus_tag="PK198_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRF37794.1"
                     /translation="MPNQVNVSSEIGRLKRVIVHRPDSGISRISPKRAEELLFDDIVH
                     LPRMQAEHDTFTAVLGAFLGKDNVMETRQLILEALDADGDTKLELLDLIVNYEELPKS
                     THDLLKELPHEALSEVLITGYHAEDDHILFDPIPNFIFTRDIAVVVNDHVVVTKAAKE
                     ARFRENFLTRFIFWSHPVFQEMRKAGRVINLNKVDIFPPSKRGEWVSVEGGDMMILNQ
                     DYLLIGHSERTSEHGIRSLAHYLLERGIVNNVAQINIPHDRSCMHIDTIFTQIHHDHI
                     VSFKPIVNEGLSSSVEVLRKHGKPGYYPSVQDFILEEINPSMRFILSGNGESPYQERE
                     QWTDGCNLVAIRPGVALTYDRNPKTEEAFEAAGYSVLHAYDFLKAVEAGRIQPDQLQN
                     TIITLPSSELSRARGGSHCMTCPIERVGV"
     assembly_gap    16337..16364
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16602..>17068
                     /locus_tag="PK198_03320"
     CDS             16602..>17068
                     /locus_tag="PK198_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37795.1"
                     /translation="MPTLTWRFFFSALFQPLPLQDIVDELRDFDARFVLIIHKHAAVL
                     GWWCILHFLAGTPVLLWASGFWQYFMLMGLSWAGINFAIVWKLYEHTYMRRFVRGDIL
                     KRFEVQRHVEKMMLFNVGLDAAYCFAGLYLLTLARVPDMARPELWSGFGWAVLV"
     assembly_gap    17069..17264
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17299..18435
                     /gene="galK"
                     /locus_tag="PK198_03325"
     CDS             17299..18435
                     /gene="galK"
                     /locus_tag="PK198_03325"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRF37796.1"
                     /translation="MTKEAATATDAATAQAPGRINIIGEHTDYNDGFVLPAAIGHKMT
                     VQARRNGTPSTVHLTARATQQSFSFDLRDFSPVEKGWPNYVMGVVSELQKLGGVISGF
                     DAEFESDVPIGSGMSSSAALECSFALALNELFGLGFDQWQLIKASQRAEHNFVGNKCG
                     IMDQFAGVMGKKDHVMLLDCRSLDFQYFPLELGAYQLLLLNTNVSHSLASSEYNTRRA
                     ECEQGVHILQQEYPGIANLRDVNLKQIAAMEEQLPEHVFRRCRHVVSENQRVLDATKA
                     LLAGDFNAVGELMYQSHFSLQNDYEVSCPELDFLVQQTLDKDYVLGSRMMGGGFGGCT
                     ISIVEKERVAELVEEVSSVYSRHFGIDLTPVEVVIGDGAKVIRQ"
     gene            complement(18492..20117)
                     /locus_tag="PK198_03330"
     CDS             complement(18492..20117)
                     /locus_tag="PK198_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37797.1"
                     /translation="MKNNYLLLLVACILASSSLFGQRLKDKRAQINYVSLPTKKVPDS
                     YTTYSVQVYGSAVTDAGHSNQALENKIVMDGFKRVNYQEGGGHLRIVMNMGYTTQGRA
                     EYKSNKITKKDEKTGVETVTWEYWYEVPLNLSAVFNIYDPAGNILTTYSKNFAGKKST
                     SRYSKSDDLSKNYNANINRMRKEVALEVINDLMREVVPIVSDQFDFDKRHDNPEFFII
                     ENYPSEPDYQKYFELLDTEWKKIDSAVPTSELKAKFGPALAFYEKEAEIDAKGDKKLL
                     KVFEAANYNAALLNFYLDDFEKAVRYANRVIASEGGKHRKSSDLIERVQKTKQLMDMH
                     GVNTLHYARDLSNAIPPAAMKALEKAQEQLESDNNLLRGVIVIKGETIEGTVVSEKGV
                     EELDFSDKGNTKFMDASKKEYSYSAKEIEAFSIGTRNFVKKNFAPCAKGKVEPTTHIL
                     EEIYTSDKIVLYKYYPSAGALSDAKMEFAFKKASDTDPISLYDTRFLILKKGLATYFS
                     DCADLKAMCEQGEITNDQEGLLKAARIYAELCE"
     gene            20502..>21650
                     /gene="argS"
                     /locus_tag="PK198_03335"
     CDS             20502..>21650
                     /gene="argS"
                     /locus_tag="PK198_03335"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRF37798.1"
                     /translation="MDIIKNIQQAAAEGVTALFGQAVQPQDIALTPTRKEFAGDYTIV
                     VFPFTKFVKKSPDAIGQELGEYLKTHLPAVADFNVVKGFLNIVLSDTVWSGFLEEHFA
                     QADYGLHASNGEKVMVEYCSPNTNKPLHLGHVRNILLGESTARILEAAGYDVVRVQII
                     NDRGIAICKSMLAWQKFSGGATPESTGIKSDHFVGDYYVEFEKRFREEYAEWQQGETA
                     GSVYEAQKKEGQSPEQFFKDYKDKYFNEHSALGREASEMLRSWESGDPETVALWKKMN
                     GWVYAGFEETLAAIGASFDKYYYESETYLLGKGMVEKGLAEGVFHKKDDGSVWVNLED
                     VGMDQKVLLRRDGTSVYITQDLGTARLRYDDFGISRMAYVVADEQNYHF"
     assembly_gap    21651..21682
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21683..22354
                     /locus_tag="PK198_03340"
     CDS             <21683..22354
                     /locus_tag="PK198_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HRF37799.1"
                     /translation="QEPYSGGLYHLAYGMVNLPSGRMKSREGTVVDADDLMAEVIAEA
                     RAGSQERGALADLSPEEQESIVRRIGLAALKFHIIKVTPRKSMVFDPTESVDLQGQTG
                     PYIQNAYVRIRSVLRKAEEDGMGGSASQEYAVLQEQEKELIRSLYEFPDLVARAAAEY
                     DPSHIAGYAYNLAKAFHKFYHDHSILRAESEAAKAFRLKLSRMVAQVLQTAMGMIGIE
                     MPEKM"
BASE COUNT         5098 a         5886 c         5845 g         5127 t
ORIGIN      
        1 cgtgatcgct gccgacaaca gcgccacgtt ttatatgatc aacatgattc cgcaggcgcc
       61 gcaggtcaat caaggtccct gggaaaaatt ggagacgtat tgccgctcgc tggcacgccg
      121 gggcttcgaa ttgtacatca tcaccggcag ctacggcacc ggcggcacgg gcaacaatgg
      181 ttatgccacc accattgcgg gcggtaaagt agccgtgccg gcagccgcct ggaaagccat
      241 tctgatactg cccatcggcc aaaacgactt gagccgcgta acggaaaaca cacgactcat
      301 cactgttgcc attcccaatg aaaacaccgc cgccaacctg tcctggggca actatcgcac
      361 tacggtaaat cacgacgggc tacgacctgt tttcggcgct gccggcttct attcagagcg
      421 ttttggaggc cagaacagac accggcccta ccgaagaata agcttactgt cggttggttt
      481 tcagcttgcc gtctcgctca tgtcgggcca tgcccaattc caccaattgg gtgatgaggt
      541 cggagtaggc cagcccggtc gcctcccaca tttttgggta catgctgatt tcggtgaagc
      601 cgggcagcgt attgatttcg ttgagaacga aggagttatc tgatcgcagg aaaaagtcca
      661 ctctggctaa gccttcgcaa catgcaattt gaaacgcctg cacggccgtc tgtcggattt
      721 ggtcgctcac ggcagggtca atatcggcag gaatgttcag cagcgcgccg tcggcatcct
      781 tgtatttggc ctcgtaggaa tagaaatctt taaccggaat gatttcgccg agcacggaag
      841 ccctggggtg ctcattgcct aaaacggcgc attccacctc tttgccgatg acggcctctt
      901 cgatgaggat tttatgatcg aatttgaagg cttcggcaag agctttgtga aagccggctt
      961 catccgtcac cttgctgaca cctacggacg aacccgcgtt ggagggcttt acaaataagg
     1021 gcaatcccaa tttttccacc acttgcgcaa agctgagcga caatggctcg tgccggaaca
     1081 gcgtgatgga atcggccagc ggaatgtgga aatcccggag caggcgtttg gtcacgtctt
     1141 tgtccataca caccgctgaa cccagcacat caggtcccac gaaggggatg tctaaagacc
     1201 tgaaaaagcc ctgcataacg ccgtcttcgc cattggtgcc gtgaatgatg gaaaaagcgg
     1261 catcaaaagt gtctaatatt tcaagattgg ttttggaaat aatggcggcg cggccctcgc
     1321 ggttgtccaa ccaaaccggc tgtgcctgag cgaaataatc attttggggc agattcacct
     1381 gatgggtcgg atactccggg ttgtactgcc agcggccttg cggatcaacg ccgagcagaa
     1441 tggcatcgaa ctggtgcagg tctatggcat tgaaaatatt ggaagcagag ttgagcgaga
     1501 cttcgtgttc ggcagatttg cccccaaaga cgacggcgat ttttttcttc ataatgtcgg
     1561 cttgaatatg gggcaaatgt aggaacgttt tggagaattt tatcccaaac ctatacagcc
     1621 ggtcccccca agctacgagc tacgagtctg ttttttcatt cagaaattag attttccatt
     1681 tggggggatt tcaaaatctc ctattggaaa taaggttctt ttttattctg gaaaatcaaa
     1741 ctcgtgactc gaagctcgcg gctagcagcc ttttgcaggg cgactgaata gtatcgcccc
     1801 caaacaggtt cctgtgtatc ggctccgttg cctcaaaaca acatcgtccc cgacaggcgc
     1861 acgtttcttt gcatggcttg caggctggaa tccggcagca gatcgttcag gccgtgctga
     1921 tagtgcagcc caaccgagaa ttttggcgtg agccggcaac gcaaacccgc tgacgccagc
     1981 agttcccaac gagccaccga attggacacc gcactgctga tctgcgcctg actgaaattg
     2041 gcagtagggg acgagcctgt gttggtgagc tgatccattt cgctggagga ttctactaca
     2101 ttttgcaggt tcagcaacaa gcctgctgta acgccgcctt ccacgcttag gcgcgggctg
     2161 agataatagc tcaacatggc cggcaactgc aaggtaactg cctgaaagcg ataagtttgg
     2221 cccgaaaaag cgcggctttc caaggcttgc agaggggtgc tgttgtcaag catgccgcta
     2281 ttgggatcgt ttaccaaaga ctgccgggct gcggtggaca taaacaagtc accggtgtta
     2341 ctgcctgcca gcaatccgct gcgggcagag atcatggtgc caggtttcca aattgctacc
     2401 gcgcccgccg aaaaattgcc cacactgcca gatcctgctg cgattccggt ggccaattct
     2461 actccaaagc gcactttttt gcgcgcattt cgcttggttt ttatggtggg ttcgaggccg
     2521 aggtcggggc gggcgttcag aaggccggct ggaactgcgg actgcaacat gggcgatgcg
     2581 cctgcccagc gtttcacgga cggagcggta agggcaacct gctgaacctg agttgattct
     2641 acagcgggcg ccgcctcggt gaaggtctgc gatgcagcag attcaatata ctcggacgca
     2701 gtctgcatcg tggcggccat ggtgtgctgt tgggcagccg gtgaaagtgt gagtgtcgcc
     2761 cagcttggag gactcacggt tttcatctcg gcaacagaag tgctttccga tgctgaaata
     2821 acggtctcag ttgggcgctc tacctgtgcc atcaccggct tgagcgcttc cacagcaggc
     2881 ttttcgggca gttgctcctg tgccagacgt tggtgttggt aaatggtggc tgctgccccg
     2941 gccgtgatga tgaaccctgc caaaagccag agccagatca ggccccgtct tttgcggcta
     3001 ttcggcgctg cggaggcctg cgcgggcatt tcggcatcca gaagggcggc catgcgttcc
     3061 caacccagat cggcgggtga ggaggaagag gtatgatgtg tatatttatc ctgcatgata
     3121 attcacattg tagtgaaatt gaagcaattc tctcagtttt tggcgcgcca tcgaaaggtg
     3181 ccatttggaa gtaccctcgc tgatgttgag gcgtttggca atttcggcat gtgcgtagcc
     3241 ttctatggcg tacatccaga agacctcccg cgaggaattg gggagcatat ccaccaattt
     3301 gacgatgtct tcaaaatata aatcgttgag tacgttgccg ttttgcggcg cgtcgcggtc
     3361 ttctatgtct aaaaagtgta cctgtttggg ctgtcgccga aagtggtcgg acagggcatg
     3421 aaacaccaac ctgcgaatcc agccttcgag cgagccgctg aaagtgaagg tatccaattt
     3481 tttgaaaacc ctcaaaaagc cgttgttcac aatttccagc gccagatcgc ggtcgtcgga
     3541 gtaccggcaa accatgcggt acatggtatc gaaatgcttg cggtacaaag cttcctgaca
     3601 tcgtcggtca ttgcggatgc agccttccac cagctctttt tcggtatatg ttttttgctc
     3661 ggccataata gtggttggga ggtatcaaaa catagcgccg aggcgcaggg tgaagcgtct
     3721 gaaatccact tttctcactt ctgtctgatc aaaaaagtca aattcgcggg tgtaagtggc
     3781 ggtttgaaaa atgtagccca catcgagcat caggttcagg tctttgtcgg cgcccaatct
     3841 caggcctact gccggatgga aacgcaggcc gccttctgcc ttggtaatga agttgttgcg
     3901 attgcggaaa gcaaaaccgt aacccgctgc gaaagacaca aaaggcgctg ttttggtttt
     3961 tgtcagaaag ctctgcgcct ctccatacac cgatgccacg ttttcgccgc cttcgggatt
     4021 gaaggtgtcg aagcccagcc cgatgcctgc ccccaacata cgattgaact gaaaaccggt
     4081 gaggttgtgc aaacctgccc caaactgcgc ctcgcccaga tacacgccgt tgggcatggt
     4141 aaacgaagtg cgattgaacc agccccgctc gcggaaagca tactcttttt tctctttggg
     4201 tttctttgtc atcgagatat agtggtagtc ataagcctgt tgcgctgtac tgcccgattc
     4261 cagtgtgggc ttgctgtctg ccggttggtt gcgttgcacg gctttgattt ctttggcgtt
     4321 taacgtaact ttttcgccgt ttttcaactt cagtatggtt tgcgtcggcg tttccgatac
     4381 gatttcgcct ttaaaaatgc tgccgttgta caaaaacaag aaatcaaccg tgccgtctgt
     4441 tttgccctga ttgctgaacg gctcctgctg gattttggcc acctcgctgt cctgaaaagt
     4501 gacctcgcgg ccgttcccga tgtctatgac caactgcccg ccgggccggt agtccaaaat
     4561 gacgcctttg atgacggcgc cgtttttcag atacaccacg tcttgcatgg gcgtttgtgc
     4621 ctgcatggcc aaggggatgc acaacaagaa cacaatcagt tggatgtaac gcatgatgta
     4681 ggattttcgt cagaagaaaa aagttgccgg ctcactcaat atgaacaccg nnnnnnnnnn
     4741 nnnattatct attgattccc aagacgctca gcttgcgcaa attggttggg tcggaggcgt
     4801 caaattgatg aaaaccgtct tcgccgatga ccatcgcaac gttgttgtgc ggcaacacga
     4861 taacgtcgaa ggcattgaat cctttttcgt ggtcgagcag attctgggtg atcttgttca
     4921 cgtccgaggc atcaaacacc ttcaaaccct cggttccttc acaaatataa agcacatcgt
     4981 tcaccaccga caggccgtgc gggttggtca tcgcgtacga cttgatgagg cgcggattgc
     5041 gcaggtcgga aatattgatg acatccaact gattggtaaa attttggcac acagtgccgc
     5101 ctctcagcgt cacatacgct atgtcgccgc tgacgaacac cgggtcgcag gcttgcgcat
     5161 gttggaaacg gctcaactgg gccggttgta gcggattggc aatgctgtaa ataaacatgc
     5221 cgttgttgga gccaatgaac aggttgtcgc catggggaaa aatggtttct atgccccagc
     5281 ctacgctcac taccgctgcc tgtgtggggg ccgtagcctg tgtgaggtcg aacacgcgca
     5341 ggttgtattc atccactaca tacaaatact tggttgctgc aatggtgaaa cgcgccatag
     5401 agccgccgat gcccaccgca gaaggcgcga aggaagcgcc gcccgaactg ctttgcagat
     5461 tgaccccgct aaagctggcc atgtccatca taatgccgcc tctttcccag aagaagatgc
     5521 ccatattctg gtcgcagggt accacgcggg tggtgggcgt ctcgcgatac tctacggtgt
     5581 agcccaaacc ctgcgtaaac ggaaacgcat tgggaaaaac gctctcggtg cggctcagat
     5641 acaccggatt ggaaggcgtg gtgatgtcca aactcaccaa atccacatag ttgtcggcgt
     5701 acaaaatgtt gttgcgcacc gccatatcca cgttgccggg aaggttgata aaagcgatgg
     5761 gcgtggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnctggtag taatagattt taccgggatt ctccaatgcg cggggcgctt
     5881 ccgtcacaat gggttggcgc atttcttccg gcagtttgta caccggctca aacaaggtat
     5941 aagtagtgat agaaacgcac ttgtcctgca agcagccgcc cagcgtgagc atggccgaca
     6001 aagcaagcgc taccgataaa atgctgaaaa tagtaccctt tttcatggtg tgaatttttg
     6061 gttgtaaatg tacaatgttt cttctctgcc tgcaaagacc gatggggtag gggaaagggt
     6121 tggggttgtg caaagatttt ttcggggcgc cccccgccgc aggccatcgt cttatggcgg
     6181 nnnnnnnnnn nnnnnnnnnn nnngccgcca ctccgccgaa aagatgctgc tacatataat
     6241 atggtgaacg aatcggcgca gtggggcatc cgttactgtc actggcgcaa gcgtggaggg
     6301 gaaacaaaaa agcccttgca accatctgat ttgcaagagc tttttgattt ttgtggagac
     6361 ggcgagagtc gaactcgcgt ccagagaaag caccagaaag ccttctacat gcttagtgat
     6421 ctgtttgaat ctcaattgtc ggggaggtca gttcaccaac ccattccgcc aatccagccc
     6481 tgaatctcga aacctgcacc gggccaatgc agttttccag ccgaaacggt tatgatgtat
     6541 gagacgcggg ccagttcggc aaaggcgggt gctcacgtag agcaggcatc gtcatacaat
     6601 ggtgcgctaa ttcctagaat taggcagcca tagcgagctt gtagtcgcca aatatggttg
     6661 atcgctattt gttaacgggg taaacaatcc tgccccggca tgcttactga ccagctcact
     6721 ctcctgtcga tgccaatacg tccccttttt ttgggcgatt gacttccgca agggaagcta
     6781 cccggcgtgt gaggcgcggc tgctcttttg cattccgttt cacacagcgc taaaaacgnn
     6841 nnnnnnnnnn nnnnnaaaaa gtttcctgat cggttaacaa aaatcccccg gcaaggcggt
     6901 aactttgccc gtcaccaacc aaataaagct cccatgaaat taggcatcat ccgggaaggc
     6961 aaagtgccgc ccgattcccg cacgccgctc acgcccgaac agtgcgccca cctcatcaga
     7021 gataatcgct tggatgtcag ggtagaacct tcgcccattc gttgttatcc caatgcggaa
     7081 tacaccgact tccacgttcc gctctcgcac gacatgcacg attgcgacgt gctcatgggc
     7141 atcaaagaag taccgattga ccaactcatt cccggcaaaa cttacctctt tttttcgcac
     7201 accatcaaag cgcaggccta caatcgcaaa ctcctgcaag ccatcttggc caaaaacatc
     7261 cgcctgctcg actacgaagt gatgaccgac gagcggggcc gtcgcctcat cgcgttcggc
     7321 aaatttgccg gcatggtagg cgcgcacaat gccctctgga cttggggcca acgcacgggc
     7381 gccttctcgt tgccccgcat gaaggattgt ttcgactacg aagccgccaa agaacactac
     7441 caccacatac aatggccgtc gctgaaaatt gtcgtcacgg ggtcggggcg cgttggcacc
     7501 ggcaccatcg aggtgctgca cgatatgggc attttgcagg tcagcccgga ggagttttta
     7561 gaaaatgaat ttgacgaggc ggtgtttaca caaataacgc cttatcaata cgttgcgcgc
     7621 aaagacggcg agcatttcca cacccgcgat ttctatgcgc atcccgattt gtaccaaagc
     7681 atttttatgc ctttcgcggc ggcttccgac attatgatca acggcatttt ctgggacaag
     7741 cgcgccccgg cattttttac cctcgacgat atgcgccgcc ccgattttcg catccgcgtc
     7801 attgccgacg tcacctgcga catagcgccg gagtcctcta ttcctgccac cgtcaggccc
     7861 tcgtccatcg ccaatccggt gtttggtttc gatcctgttt cggagcagga aatccccgct
     7921 ttcgattcgc gtggcataga cgtgatggcc atagacaacc tgcccagcga aatgccccgc
     7981 gatgcctcgc gcgctttcgg cgatatgttc atcagccata ttttgccgga attgctcttg
     8041 cccggcagca aggttgtcga gcgtgccact gtggctgtgg atggccaact tgggccgcat
     8101 ttccagtatt tgcaggagta tgccggtttg gtggaggttt aaggtgccgg cggcttcagc
     8161 cgccgtattc ttcgtgcgaa cttgttcgcg cgatctgact ctctgcccgc gcgaggttct
     8221 acctcgcgtg ggctatcctg cgggttccac ccgcaagcga actcttcaac cgtccatatt
     8281 ttcgcacaac atgccataca tctcacactc cacctaccac ccgccgctgc cctttcggca
     8341 gggccatatc aacaccgtct ttgccgccct cggccgtcgc ctgcccgcca tgccttacat
     8401 cagagagcgc tgggacaccc ccgacggcga ttttttagat gtggactggg ccgctcaggg
     8461 caattcccgc cgcgtgctgc tggccctgca cggcatggaa ggcgacacct cgcggcctta
     8521 cgtccggggc atcctgcggg catttgccat gcacggctgg gacgcgctgt cgttcaattt
     8581 ccggggttgc agcggcgaat ccaaccgcct gccccgcgcc taccacatgg gcgaaaccgg
     8641 cgatctggat ttcgttttgc acgaactctt gcaacgccac gattacgatg aagtagccct
     8701 cgtgggcttc agtctgggcg gcaacgtgct gctcaaatac ctcggcgagc aaggccgcgc
     8761 actgcatccg gccgtaaaaa aagcagtcgc tttcagcgtg ccctgccaca ttccctcctc
     8821 cgaacgcgcc atcgcccgcc cgatcaaccg gccgtatgtg tggcgatttt tgcgcaccct
     8881 caaccccaaa atgcaacgca aagccgccct gtttccgggc ctcatcaacg ccgcgccgcc
     8941 attgccccgc agcctgcgcg aatttgacga gcgcttcacc gctccgctgc acggctttgc
     9001 cggcgccgaa gactactggc agcgcagcag cagcttgccc tggattgaaa aaataaccgt
     9061 gcccaccttg ttggtcaatg cgcaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnggg gtagagggtt cctaaaaaaa taaaaaaccc caccccatac tactttgccg
     9301 gaaaacgcta cctttgcgct tcgcagaaaa aagcccgtag cttgttaagc aaggtaaacg
     9361 ggttatggtt taacgtaaac ctttgattta caatggctgt taaaatcaga ttacagcgta
     9421 aagggcgcaa acaagcgccg ttttatcaca tcgtggttgc ggattcccgg tctccccgcg
     9481 atggtcgctt cattgaaaag atcggtactt ataacccgct gacgcgtccg gcaacgattg
     9541 acattgaccg cgacgcagct ttcaactggg ttatgaacgg cgctcaaccc accgacacgg
     9601 cccgcgccat cctccgcttc aaaggcgttt attatcgcaa acacctcgca ttgggcgttg
     9661 ccaaaggcgc gctcacgcag gaagatgccg acaagcgcta tcaggcatgg gtggaagcca
     9721 aagagtcgcg tatcgccgct cgcgctgcca aaacagccga ggaaaaagct gcattctatg
     9781 ctatgatatc cggccaaccc aagcccaaag ccgcaccggc tcctgccgtt gtagagcctg
     9841 aagccgaagt ggctgaagaa gtagcagaag ctgccgaaga agtagtggaa gcagtagtgg
     9901 aaactgctga agaagccgct gtcgaggccg ctgaaaccga cacagaacaa gcttgatgag
     9961 cgtgtatcgc tcctgaaaga gctatacttt aactctgtag cgaacctggt agtgcctcaa
    10021 taggtgctat caggtttgcc tttaaaaaac ggtaagctat gtacgaatta aatcaaaaat
    10081 acatcgagca attggaactg attgccgaga acattcaaga ctccgaagaa cttgctcaat
    10141 acctcgaaga agaggaagaa gagttttata atcagctcaa agaagcttac gagccgcgcc
    10201 tcgccgagat gtatcaggag gtggctcata ccaatcccct tcagttggtg tcgttggagc
    10261 ttgtactgct cgatccggct ttcgaagggc ttttcctgcc gcgcatttta gggtactcgg
    10321 tacttcgtgg ccagatcaac gagcattaca aatacgttcg tccgcaggag catttcaaac
    10381 agattttgct cactatttgc gactcctcca acttcgatct gctcaaaaag cgcatcggtc
    10441 aaaccatcca aatcggtttt gccctcagca gtgatatctg gatcacgaat ctcattgcag
    10501 gcatcaccaa caaaaaggtg tcgaattacc ttcaggcgca aaaattagag cgctttttgg
    10561 atgaaaatga gcgcaaaaac ggctatgttc gttatgcccg ccagttccac aacgaaaatt
    10621 acctgagtgc ggatttcccc accgaccgta cctcgctgct gctcaatgct gcacagctca
    10681 aacgtttcct cacttaccgc atttctgcca aagttgacaa ctccaatctc atcaaccata
    10741 ttgacgactt ggtggcgcat gaggattttt acggcgtgcg cgaacacttt gaactcatgt
    10801 tgttgtacgg ctgcttcttc gagcacgatg aggcgtcgga ggctattctg cgcgaagtgg
    10861 tgaacgatgt gcgccgcaat cacccggagg tggcacagtg gggaatggaa tatgtgcggg
    10921 aattgcatcg ccattcgcag ctcgacctgg ggccggccaa tgagaagcgt tttgcagctc
    10981 tgttggacaa atccatcaac gacgaattgg cggagtattt ccaattggcc gacaccgtgc
    11041 ataccaaagg ttataccaac atagaggcac aggatgccgt caaggaattc tacctgcaac
    11101 acgaaggact ctccgtagcc aatgagtgtg tgcgccagat gattttccgc tatttccgca
    11161 gcttcatcaa caatctggaa aacgacgctt ataccgactt cttcgagatt tgccgcctct
    11221 tccaggtgta tatgaatatt ttctccaacc agcaattcaa tcaggatttg gaggatttgt
    11281 caatggaata tgtcgggaaa ttgctgctgc attacacaga caaacgcggc aaagattatc
    11341 aggacatcaa gcgtttcgtg tcttccacgc tgcaggattt ccgtttcgtc accgaaaaag
    11401 aggtggtgga aatgttcaaa accaaacgca agaaaaagga agattgattt tatacgcccg
    11461 cctgcccgta gagacagagc atgtcctgtc tctacgggtt atgcgggtgc gcgatcacca
    11521 aacaacaagg taccgatgcg caccatcgta ctgccggttt caatcgcaac cgggtaatcg
    11581 tcggacatgc ccattgaaag gtgccggaag tgttcgtttt caaagaaaaa ccgctctctc
    11641 agccgcccga aaatgcgacg cagcaactga aactcgcccc gaacctgttc catatcctcg
    11701 gtaaaagtag ccattcccat gacgccgcaa atgcgcacat tcggccaagc ttcgaactga
    11761 ttttgcgacc atgagtcggg cgcatcgggc agcaacccgt acttactgct ttcctgcgcg
    11821 atgtggaatt gcaggaggca gtcaatactg cgatggtttt tagcggcttg tttgtcaatt
    11881 tcgttcaaca gatcaaggct gtcaaccgaa tggatgagan nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnatttc actttgttgc tttgcaaatg cccgatgagg tgccagcgaa tgtctttggg
    12001 cagggcttcg tatttgggta gtaattcctg cacccggttt tcaccaaaat cgcgttgccc
    12061 ctgccggtac agttcgagaa tagcctgttc tggttgggtt ttcgagacgg ccaccaattg
    12121 tacttccttg gccctcagtt cgttcaaaat agatggatac aaattgctca cggcgtagtg
    12181 tttggcctgt aacgtttggc atcgagagag ggttgagcat taaatggcac aaaactctgc
    12241 ggcagttgtc tcaaatccct tttctttgca ttgaatcacc acatcgannn nnnnnnnnnn
    12301 nnnnnnnnna atcatgctca aagcaggcat caccggcgga ataggcagcg gcaaaagtac
    12361 cgtttgccgg gtttttgaat ggctcggcgt tccggtctat tatgccgacg accgcgccaa
    12421 acaactcatg acggaggacg cggcgctgcg ctccgaaatt cgcatacttt ttggcgacga
    12481 agcctacctg ccagacggtt cgctgaaccg ggcgcacatt gccggcatcg tcttctccga
    12541 cgcggatatg ctccggcaac tcaacgcatt ggttcaccca gccgtacacc gcgacggagc
    12601 ggcatggcac ctgcgacaaa cggcgcccta caccctgcgc gaagcggccc tgctgtacga
    12661 gagcggcggc taccggttgt tggacaaaat ggtggctgtg acggcgcccg cagaattgcg
    12721 catagagcgg gtgatggccc gcgacggggt agatcgcagc gcggtagagg cgcggctgaa
    12781 caagcaatgg ccggaagaga aaaaagtgga aatggctgat tttgtgattg ttaatgacgg
    12841 aacaacgccg ttgctgccgc aaattttgca ggtgcatcgg gcgctgtctt tagaagcaga
    12901 taagtagcag catgtcaaat acaccagaag aagcccgcat cgaagcggaa gttcaaaagc
    12961 gcctggcagg catcatggcg gagcagcaac agcaatgggc tgcagcgatg gaagctatga
    13021 tgaaaaactc agtgggaggg gttgagcaag ccaataccgc ccttgccaaa gagcaaaaaa
    13081 agctcgaacg cgaactcgac cgcgcccgcg aactccacag aaaagctgaa ttggagggcg
    13141 aaaaaatggc gatggatgcc tacgaaaaac accgcgcaca atacgacgag tcggccaaaa
    13201 taaaagtgct gcgcgacttg accagtctgc acattgaagc gggcaagacc gacgaggaaa
    13261 tcatcgcgtg gatcggcgtg gagcaagatt ttatagaaaa cattcgcgtg gtggtgcagc
    13321 gggtacaaaa atttcgtgca gaaaaccaag ccaagcgcgt cattccctcc ggccggcctg
    13381 tggttcggtt tcaggattac gggcgcagcg gcacggtgta ttttgagagc gaggaggcca
    13441 agtttgattt gtggtgggaa atgggcgttc atgccctgat cattgtggat gcgcccactc
    13501 ccgatatgtg ggaagacaaa accggattgc cgcctgaacg ccgcgccgaa attctgaatt
    13561 tcatcggcga ggaaatcgtg gctacgcaaa ccatcagtgg cggttccttc atcgtgggcg
    13621 aaaacgtgct gacgatttat gtggattgat tttgggagca tcctatcttt acccccgaaa
    13681 tattcttttt ttcattcaaa aaccacccat catcgcagca acggcatcat acgactttct
    13741 catcgtcgga ggcggactct tcggcgtgta tgcagctctg tatctctccg ggcacggctg
    13801 ttccgttgct ttggtggaaa tggaagacga attgctcaaa aaagcctcca tcgtcaatca
    13861 ggcacggctg cacagcggct atcactaccc gcgcagcgtg gccaccgcgc gcatgagcga
    13921 cgatcacaaa gagcgtttca tcgccgatca ccggcctttc gtgctgttcg attttgaaaa
    13981 atactacgcc attgacaagt acggctcttt caccgacgcg gggcagtttg agcgattttg
    14041 cgactttatc aacattcggc gcgacaaggt ggaggacacg cacctctggt tcaatttcga
    14101 ccgcatcgaa gcgctatatg ccaccaccga atacaccttt gatcctgtac tgatagccga
    14161 tcattaccgg caacgcattg aaaaacaaaa gggtgtgacg attttcaaaa gtacggtgta
    14221 aagcaggcgg gcgcggcggg cgcagtttgg gagcttgttt tggaaaaccg gcacacacaa
    14281 caaacgaccg ctttgcaggc cgctcaggtc atcaacgcca cctacgcggg cagcaatgcc
    14341 gtgaaccgta ccttcggcgt ggacgacatc tcgctgatgc acgaaatctc ggaaatcgcc
    14401 ttcgtcacct ccccgcagat cgggcgcatc ggcctcaccg tgatggacgg gcagttcggc
    14461 tccatcatgc cgtacgggct ttccggcctg ctgtctttgt catcggtggc ctacacgcac
    14521 cacagggttt cttacgacaa cctgcccacg tttcactgcc agtcgcaggc gccgaactgt
    14581 cgcccgcagc agcccggcaa ttgtaatttc tgcccggcgc agcccccctc caacgccgcc
    14641 aaaatgatgg cgcagatcaa acattacttc cgaccggaag tggaattgcg ctactatttc
    14701 agtcttttta ccatcaaaag caagctgaaa gccagctata tagacgacgg aagacccacg
    14761 gaaatcagtt gcctgaatga gcatccgcga tattattgca tctttgcagg taagatcaat
    14821 tccatttatg agatagaaaa gatcattgtt ttttaaacgc tctgaccaat catgccaaac
    14881 caagtaaacg tctcttccga aatcggaagg ctcaaacggg ttattgtaca ccggcccgat
    14941 tccggcatct cccgcatctc gcccaaacgc gccgaagaac tccttttcga cgacatcgtt
    15001 cacctgcccc gaatgcaggc cgaacacgac accttcaccg ccgtgctggg cgcttttctc
    15061 ggcaaggaca acgtgatgga aacccggcaa ctcatcctcg aagcactgga tgccgacgga
    15121 gataccaagc tggagctttt agacctcatt gtgaactatg aggaactgcc caagtccacc
    15181 cacgacctgc tcaaagaact gccgcatgag gcgctctccg aagtgctcat caccggctac
    15241 cacgcagagg acgatcacat cctgtttgat cccatcccga atttcatttt cacccgcgat
    15301 attgccgtgg tggtcaacga ccatgtggtg gtcaccaaag ccgccaaaga ggcccgtttc
    15361 cgggaaaact tcctcacgcg gttcatcttc tggtcgcacc cggtatttca ggaaatgcgc
    15421 aaggccggca gggtcatcaa cctcaacaaa gtggatattt ttccgccttc caaacgcggc
    15481 gaatgggtct cggtggaggg cggcgatatg atgattctca atcaggacta cctgctcatc
    15541 ggccacagcg agcgcacctc cgaacacggc atccgctcct tggcgcatta cctgctggag
    15601 cgcggcatcg tcaacaatgt ggcgcagatc aacatccctc acgaccgctc ctgcatgcac
    15661 attgatacca tttttactca gatacaccac gatcatatcg tgtcgtttaa acccattgta
    15721 aacgagggac ttagctccag tgtggaagtg ttgcgcaagc atggcaaacc ggggtattac
    15781 ccttctgtgc aggattttat tctggaagaa atcaacccat ccatgcgctt catcctcagc
    15841 ggcaacggag aatcgcccta ccaggaacgc gagcaatgga ccgacggctg caacctcgtg
    15901 gccatccgtc ccggtgtggc gctcacctac gaccgcaacc ccaaaaccga agaggctttt
    15961 gaggcggcgg gatacagcgt cctgcatgcg tatgatttcc tcaaagcggt ggaggccggc
    16021 agaatccagc ccgatcagtt gcaaaacacc atcattacgt tgccttccag cgagttatcg
    16081 cgggccaggg gcggttcgca ttgcatgact tgtccgattg agagggtggg ggtgtgatgg
    16141 ggtatctaag cacaggctac aagctgctgg ctacaggcca caagccataa gctgcttgat
    16201 atcttcgtcc gaataaatat cctcttgttc attggccaga aaacccagtg cgccactgtc
    16261 ttgcaccata ttggcaattt cgattgcgga aatgtcatca tccggggtat tgctgtctaa
    16321 atacggaaaa acgacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggat gttcactttt
    16381 tccagcaatt gcaactgatg cccgtcgtat aatgctctta cgctgaccat atatcgtttt
    16441 tgtcctacaa caatacagcc tttgaagtgg ttgacgccaa cccttcttcg gtcgccttcc
    16501 cagtatcact ttttacacac attttacagc cccaaaggct gggtgggcgg gcagacgcgg
    16561 taactttgcc ccacattttc gctacaccaa aaccgcctgc catgccgacg ctcacctggc
    16621 gctttttctt cagcgccttg ttccaaccgc ttccattaca agacattgtg gacgagttgc
    16681 gggatttcga cgctcgcttc gtgctcatca ttcacaagca cgcggccgta ttgggctggt
    16741 ggtgtatcct gcattttctg gcgggcactc cggtgctgct ttgggcatct ggtttttggc
    16801 agtacttcat gctcatgggg ctgagttggg ccggtatcaa ttttgccatt gtgtggaaac
    16861 tgtacgagca cacttacatg cgccggtttg tgcgcggcga tattttgaaa cgctttgaag
    16921 tgcagcgcca tgtggaaaaa atgatgctct tcaatgtggg tttggatgcg gcttactgct
    16981 ttgccggcct ttatttgctc accctggctc gcgtgcccga catggcgcgc ccggagttgt
    17041 ggtcgggatt cggctgggcg gtgttggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgtt gccatctttg
    17281 atgataaaat aaacagacat gaccaaagaa gcagcaaccg ccacagacgc cgccactgcg
    17341 caagcccccg gccgcatcaa catcatcggc gagcataccg actacaacga cggctttgtg
    17401 ctgcccgccg ccattggcca caaaatgacc gtgcaggcac gccgcaacgg cacaccctct
    17461 acggtgcatc tgaccgcccg cgccacacag cagtcttttt cattcgacct ccgcgacttt
    17521 tcacccgtag aaaaaggctg gcccaactac gtcatgggcg tcgttagtga attgcaaaaa
    17581 ttgggcggcg tcatcagcgg tttcgacgcc gaatttgaga gcgatgtgcc catcggcagc
    17641 ggtatgagtt cgtcggcggc attggaatgt agtttcgccc ttgccctcaa tgaattgttc
    17701 ggacttggat ttgatcaatg gcaactcatc aaggcttcgc agcgggccga gcacaatttt
    17761 gtcggcaaca aatgcggcat catggaccag tttgccggcg tcatgggcaa gaaagaccat
    17821 gtgatgttgt tggactgccg cagccttgat tttcagtact ttccgctcga attgggcgct
    17881 tatcaactgc tgctgctcaa caccaatgta tcgcactcgc tggcctcctc cgagtacaat
    17941 acccgccggg cagagtgcga acagggcgta catattttgc aacaggaata ccccggcatc
    18001 gcgaacctgc gggatgtcaa tctaaaacaa attgcagcta tggaagagca actgccggag
    18061 catgttttcc gccgttgtcg ccatgtcgtt tctgaaaatc agcgcgtgct ggacgccacg
    18121 aaagccttgt tggccggcga cttcaacgca gtaggcgaac tgatgtacca atcccatttc
    18181 agcctgcaaa acgactacga agtaagctgc ccggaactcg attttttggt gcagcaaacc
    18241 ctcgacaaag actatgtact cggcagccgc atgatgggcg gcggcttcgg aggttgtacc
    18301 atcagcatcg ttgaaaaaga gagggttgcg gagcttgtag aggaagtttc atccgtttac
    18361 agccggcatt tcgggataga tttgacgccg gttgaggtcg ttatcgggga tggggcaaaa
    18421 gtcatccgac aatgaaatgg gcggctcgcc caaagaccaa gctttgagtg agccgccggt
    18481 tgtgtcttcc attactcaca caactccgca taaatccgcg cagctttcag caagccctcc
    18541 tgatcgttgg tgatctcgcc ctgctcacac atggctttga ggtcggcgca atcggagaag
    18601 tacgtcgcca gacctttttt cagaatgagg aaacgcgtat cgtacagcga aatggggtcg
    18661 gtatcgctcg cttttttgaa cgcgaactcc attttggcat cgctcagcgc gccggcggaa
    18721 gggtagtatt tgtacagtac aattttgtcg gaggtgtaaa tctcttccaa aatgtgcgtc
    18781 gtcggctcca ctttgccttt ggcgcagggc gcaaaatttt tctttacaaa attgcgcgta
    18841 ccgatggaaa acgcctcaat ttctttggcc gagtagctgt actctttttt cgaggcatcc
    18901 atgaatttgg tattgccttt gtcgctgaaa tccaattctt ccacgccttt ttcgctcaca
    18961 acagtgccct caatggtctc ccccttgatg acgatcacac cgcgaagcag gttgttgtca
    19021 ctttccaatt gctcctgtgc tttttccaat gccttcatgg cggcaggcgg tattgcattg
    19081 ctcaggtcgc gggcatagtg cagggtattg acgccgtgca tgtccatgag ttgtttggtt
    19141 ttctgcacgc gttcgatgag gtcggaagat tttctgtgtt tgccgccttc ggaagcgatg
    19201 acgcgattgg catagcgcac ggctttttca aaatcgtcga ggtagaaatt gagcagggcg
    19261 gcattgtagt tggcggcttc aaagactttg agcagcttct tgtcgccttt ggcatctatt
    19321 tcagcctctt tttcgtagaa cgccagggcc gggccgaatt ttgctttcaa ctcggaggtc
    19381 ggaacggcag agtcaatttt cttccattcg gtatccagca gctcaaaata cttttgataa
    19441 tcaggctcgg agggataatt ttcgatgatg aaaaactcag gattgtcgtg tcttttatca
    19501 aaatcgaatt gatcggaaac gatggggacg acctccctca tcaaatcgtt gatgacctcc
    19561 agcgccacct ctttcctcat tcggttgatg ttggcgttgt aatttttcga taaatcatcg
    19621 gacttgctat atctggaagt ggattttttg cctgcaaaat ttttgctgta ggtcgtgagt
    19681 atattgcccg ccggatcgta aatattgaat acggcgctga ggttcagggg cacctcatac
    19741 cagtactccc aggtgacggt ttcaacgccg gttttttcgt cttttttggt gattttgttc
    19801 gacttgtact ctgcccgacc ctgagtagta tatcccatgt tcatcacgat gcgaaggtgt
    19861 ccgccgcctt cctgatagtt gacgcgcttg aagccgtcca tcacgatttt gttttccaat
    19921 gcctgattgg aatggcccgc atctgtgacg gcgctgccgt aaacctgcac ggaataagtc
    19981 gtgtaggaat cgggaacctt cttcgtgggc aaagacacat agttgatctg ggcgcgcttg
    20041 tctttgaggc gttggccgaa aaggctgctg cttgccaaaa tgcaggcgac gaggagcaaa
    20101 aggtagttgt tcttcatttg tagaaattta tttgtgaaat aatcaggtta caaagggcga
    20161 aatgggcggc gttttgcctc cagggcatcg ggagaaaagg gcttatcgca gtgttggcaa
    20221 gaagagtgta attccggcat ggaggctggt tttttcgtca ggacttttgt actgtttgat
    20281 ggtgtaaaga tagtcattgc tcgcaaaacg gcatagtttt ggtattaaaa aaatcagatt
    20341 ttttgaaaaa aaaatgactt gaaaatgggg ctttgtttca gacccggcgc gggcctgcga
    20401 gtgaatgttc tgcggggttg gccctgcaaa atagcaagcc gaccggctca aaataccgcc
    20461 gctttcttac ctttgccccc tcgctcaaaa gcgcttttac aatggacatc atcaaaaaca
    20521 tacaacaggc cgcagcagag ggcgtaacag ctctgtttgg acaagccgtt caaccgcagg
    20581 acattgccct gacgcccacc cgcaaggagt ttgccggcga ttataccatc gtcgtttttc
    20641 ccttcaccaa gtttgtaaaa aaatcgcccg acgccatcgg gcaggaactc ggcgagtacc
    20701 tcaaaacgca cttgcccgca gtggccgact tcaacgtggt caaggggttc ctcaatatag
    20761 tcctgtccga taccgtgtgg agcggctttt tggaggagca tttcgcgcag gcagattacg
    20821 gtctccatgc ctccaacggc gaaaaagtga tggtggaata ttgctcgccc aataccaaca
    20881 agcccctgca cctgggccat gtgcgcaaca tcctgctcgg cgaatccacc gcccgcatcc
    20941 tcgaagctgc cggctacgat gtggtgcgcg tgcagatcat caacgaccgg ggcatcgcca
    21001 tctgcaagag tatgctggcc tggcaaaagt tttcaggcgg cgccacgccc gaatctacag
    21061 gcatcaagag cgaccatttc gtgggcgatt attatgtgga atttgagaag cgctttcgcg
    21121 aagaatacgc cgaatggcag cagggcgaaa cggccggcag cgtgtatgaa gcccagaaaa
    21181 aagagggcca atcgccggaa cagtttttta aagattacaa agacaaatac ttcaacgagc
    21241 acagtgcgct ggggcgcgaa gcctccgaaa tgttgcgcag ttgggaatcc ggcgacccgg
    21301 aaaccgtagc gctctggaaa aaaatgaacg gctgggtgta tgccggtttt gaggaaacac
    21361 tggccgccat cggagcttct ttcgacaaat attactacga atccgagacc tacctgctgg
    21421 gcaaaggcat ggtggaaaag ggcttagccg agggcgtttt tcacaaaaaa gacgacggtt
    21481 ccgtctgggt caacctcgaa gacgtgggca tggaccaaaa ggtgctgctc cgccgcgacg
    21541 gcacctcggt ttacatcact caagacctcg gaacggcccg cctgcgctac gacgatttcg
    21601 gcatttcgcg catggcttat gtggtggccg acgagcagaa ttaccatttt nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nncaggaacc ctattccggc ggcctgtacc acttggctta
    21721 cggcatggtc aatttgcctt ccggcagaat gaaatcgcgg gaaggcaccg tggtggacgc
    21781 cgacgatctg atggcggagg tcattgccga ggcgcgggcc ggttcgcagg agcgcggcgc
    21841 tttggccgat ttatcgcccg aagagcagga atccattgtg cggcgcatcg gattggcggc
    21901 cctcaagttc cacatcatca aggtgacgcc ccgcaagagc atggtgttcg accctactga
    21961 atcggttgat ttacaggggc aaaccggtcc gtacatccaa aatgcgtatg tgcgcatccg
    22021 ctcggtattg cgcaaagcag aagaggacgg catgggcggc agcgcctcgc aggagtacgc
    22081 cgttttgcag gagcaggaga aggagttgat tcgcagtttg tacgagtttc ccgatctggt
    22141 ggcccgcgcc gctgccgagt acgatccttc gcacatcgcg gggtatgcgt acaatctggc
    22201 caaggcgttc cacaagtttt accacgatca ctccatcctc cgcgccgaaa gcgaggccgc
    22261 caaagccttc cgcctgaaac tcagtcgcat ggtggcgcag gtgctgcaaa cggcgatggg
    22321 tatgattgga attgagatgc cggagaagat gtgaggaggc aggggtgaga ggtgagtggg
    22381 tgagtggtgc cagaggtgag tgggtgagtg gtgagtggtg agtgggtgag tggtgagtgg
    22441 gtgaggagaa tcgcccctgt tttcatgcga accttcccgt ctctgagcag ggtagtgaag
    22501 gggtgactca gccggcgatt cagccccgta gtcacccctt c
//
LOCUS       DAORLF010000021        22125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1281_length_22125_cov_2.062936, whole genome shotgun sequence.
ACCESSION   DAORLF010000021 DAORLF010000000
VERSION     DAORLF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22125
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_22125_cov_2.062936"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1263)
                     /locus_tag="PK198_03345"
     CDS             complement(<1..>1263)
                     /locus_tag="PK198_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HRF37800.1"
                     /translation="IVFAHPDPNGGAVITDNIVNTDTENAIAGIWQLFSSAPALLDNN
                     TVTVGKAGMGIGSAAAAKSTLRDNTVSLSDPTAAAAGIDLANVTKSLLEDNTVTGGGT
                     GGPDNIALRIVSSAGNVYCCNTLSNTRIGAHISGGSLATDNFRGTSFSNHATSLLLPD
                     VNAILGTQTHTKNRWLQDAGPAVYEVSSNFAENLPFFVDVSSSNGDPSYEPEDFSPED
                     WFQSATDTEDMDPCDEEVCMVNGFAPESDDVKKIALGDTLISAAVLWELQRYVYERLQ
                     GASVSNPTIFEFLARSDTNSIGAFYAIQDGINAMFSADSLERNQLKYNLALVEQKLDS
                     LVHIDEQMLEADSAGIAALMPVKIQLLGDLFELSSDNKDLSEGIWSNVKSEAGKLYSQ
                     NDSISATHSFEINEKVVNGLYLNWLDND"
     assembly_gap    1264..1273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1274..>2290)
                     /locus_tag="PK198_03350"
     CDS             complement(<1274..>2290)
                     /locus_tag="PK198_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37801.1"
                     /translation="NVVISNVCGETNLNAIAETDYCPPFSCETCEESLIGEDGQTLKL
                     SVAIGNDLLPADGTDIQVCVKGTLLIDQNYNIINSIFYMLPGSRIEIEDGSTLAIIDS
                     DFSGCDNMWYAIEVQQGGGMKMIGNRVSDAQYAVFVHSYPGNDPLTGGVSIYDNDFDN
                     NFVGVLAGGASPGSVNAAVGSNRYTQTAASLLPPFSGQSSAPEGLPEQNQHALAGIVA
                     DNLANFNSLNNRFEQLTSGIVARHSNLGSYMDQQISIRSNNAAYPAWDYRGRGVSIQS
                     TGVHSAKVEKGYFEDCPQGIMANNAKLKATENEMLQMEVGIWAAIGSAGGISITNNEI
                     EATRL"
     assembly_gap    2291..2304
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2305..>3267)
                     /locus_tag="PK198_03355"
     CDS             complement(<2305..>3267)
                     /locus_tag="PK198_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37802.1"
                     /translation="WFGATGTIDYYSNPTAFTGSGNVHMIRGGFGSTIESAFTPANAM
                     QGNYFFSFYTTAKHSSTMLTGNMVMNAMLVNADITALFGRGANFPSRPSQVDLNGGNY
                     SLLTSHTFPFYGSNANLNSGSFSRAALCVELAESYSSLWFYPDEGAQRLDVFLDEVEL
                     IPDDFSAGEDFVSTGCDFLGGPFCMLSDVPVRYQWFEVTGVNETLIAEYTVLNGVATV
                     VNGDVDHQTQQLFIEPEQTTTYRVRRSLTTPHNLPSSFEFCTLEDDVTVTVLPSPPMP
                     EFGVLSESCGEVTFFLNNLHPGDSYLINYGDGSSGTAIVHAYEN"
     assembly_gap    3268..3300
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3301..5248)
                     /locus_tag="PK198_03360"
     CDS             complement(<3301..5248)
                     /locus_tag="PK198_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37803.1"
                     /translation="MYAYSSNSNNNVQAVFTNVTVTQSDATPATSIAFADSLIQAQSG
                     DSILICATLQNPCVCSPVSVDIALVSDSLPHFEGYEPVTLTFSGSDTLQCFYLVAAAS
                     DTVGVYSFELSGIQGGNNTEIEGPATLTIEIEGVGEELPPGLCGAFVKDIPEIDSTKT
                     YYADRFGNLYEQEELLLPENLNSGCGCAEFTDADIPGLSASYFNLVFEDCVYNNNAGF
                     NQQLGDLGIARRKVACRVFANLSQLIIQRTSPCQGDDFSKVNIRFLPYDNSQVVSWQN
                     GPVLAAASPVFESLNYTLEGGIIDCLPWKILNNGRYTSLSGNADIFHASIGVDFTKSF
                     YTEVVPPDASAGNLFDLYDVLLHEALHAVGFFSLLNPSTGGYFSTSFTSYNRYDTFLK
                     LQNGAKVVVQDANDPYVWNLNINQGDLHTSCLGNSTENMVFPPMAGDALPIYNEMDNA
                     AFSHLDARCPNAGADYLMKPSFDRGVRVDIGEAELKILCALGYEVTQMENCSCVAAGD
                     HDLRHGCDGEPLVLELCEDALNITINLSALLENDQPNQEIGNLVNINPNIGTLEVVGG
                     SILYTPCAPGVHRFKYTPIAEDCQDGSTAFVEILVRRCFEDCSFFLSDNPINGGHNNN
                     TCNLICNPEIVVHGTTGGDVLELES"
     assembly_gap    5313..5373
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5374..5761)
                     /locus_tag="PK198_03365"
     CDS             complement(<5374..5761)
                     /locus_tag="PK198_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37804.1"
                     /translation="MSHVALSAATGSSNVFCDAWQDMSLGSGTGSSAYAACMPDTVVL
                     TTNAVTPAMATSDNHEMVYHELCGNGSLTVKIPSLSGGAAGLFVRESSAVGSRKCAVM
                     TQKGAQVFRQLRASTGMFQQQAAVNAS"
     assembly_gap    5848..5892
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6184..6627)
                     /gene="moaC"
                     /locus_tag="PK198_03370"
     CDS             complement(6184..6627)
                     /gene="moaC"
                     /locus_tag="PK198_03370"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRF37805.1"
                     /translation="MVNITHKNHTLRTAIAQAIVRAGSPETIQAVLEKRVPKGDVCEM
                     AKAAGLFAAKRTSDMIPDCHPLPVEYAAFQFDIQPDHILIIAEIHTIYKTGVEVEAMH
                     AASVAALTMYDMLKPIDKHITIEQIKLLKKTGGKSDFAVGSLRCE"
     gene            complement(6844..7272)
                     /locus_tag="PK198_03375"
     CDS             complement(6844..7272)
                     /locus_tag="PK198_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRF37806.1"
                     /translation="MDNLFVHGAITSALLAQAVEWLQSKTGLGAHSIFLGQVRADETP
                     AGPVTAIHYTAYEDMAREKAAEICAAVVEAYQLELLEIYHSLGTVKTGEISLLVLACS
                     RHRRPAIEACAAAVERLKKELPVWGQELTDQDVSFWKVNT"
     gene            complement(7265..8362)
                     /locus_tag="PK198_03380"
     CDS             complement(7265..8362)
                     /locus_tag="PK198_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRF37807.1"
                     /translation="MSAFQRQILLPDFGAAGQEKLSRARVLVVGAGGLGCPVLLYLAA
                     AGVGGIGIVDGDTVSESNLNRQVLYGPDDIGLPKAEIAARRLREQFPNTEFEALPFFM
                     DVENALETIARFDVVVDGSDNFPTRYMVNDACYLLQKPLVQGAVYQYEGQVAVFDAQR
                     GRSTYRDLYPVPPAAGDAPNCSETGVLGVLPGIIGLMQAAETIKCITGVGQPLLDKAL
                     FYHFKNASFFEVEIAPHPDAQAMMPANEAAFRAMHYADACERVQDILWTEAQALLERR
                     PETLLLDIREEEEGPNVHVRSRRWPMSRLLQDASELTTDVPLLLFCRSGVRSRHLAGV
                     LQKSGRHTQVYSIEGGLQHPLSPIHHSVTHG"
     gene            complement(8508..8765)
                     /locus_tag="PK198_03385"
     CDS             complement(8508..8765)
                     /locus_tag="PK198_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37808.1"
                     /translation="MNTFPIWEPAQSEIGAEILERFSKHQQDLKKLLEDSSDLIQKGA
                     VISSPANRNIVYTLETAFDIIVAHEQRHLEQAREIRRMLPS"
     gene            complement(8728..9045)
                     /locus_tag="PK198_03390"
     CDS             complement(8728..9045)
                     /locus_tag="PK198_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF37809.1"
                     /translation="MKWMSEIDGVTHAVKQEFASLDAGQLNWKPSPGIWSIAQNIEHL
                     IVVNETYFPVIASLKAGTYRTPFLGRLGFMVSFLGKTVLNAVQPDRRKKNEYLSDLGT
                     CSK"
     gene            complement(9042..9827)
                     /locus_tag="PK198_03395"
     CDS             complement(9042..9827)
                     /locus_tag="PK198_03395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF37810.1"
                     /translation="MRRIHALEWEDLRWFPASWRDYGTDYLRFVATKSDLYKPIVPLI
                     RKGLQASGGRHWVDCASGGGSSLIMLAKALHSEFPDLKITLTDYYPNIRAFERTQAEL
                     KNVFDFEKQPVNAMEMPPHLHGRFITLFGAFHHFRPDDARKILQDAVDTQSPIAIFEP
                     VGRNAISWFSMLFVMLNVLLFTPFIRPLRWKVLPFIYLLPLVPLYVLWDGIASILRTY
                     SEKEMKELVASLRHSDAFEWEIGKTAGALPVQYLLGIKKEHTQ"
     gene            complement(9897..10142)
                     /locus_tag="PK198_03400"
     CDS             complement(9897..10142)
                     /locus_tag="PK198_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRF37811.1"
                     /translation="MTTQPTIQLLAFGIVAEKAGSSRLSISGVSDTEQLLEALYQQHP
                     ALREVRFAIAVNRKIVHENTPLQADSEVALLPPFSGG"
     gene            complement(10419..>10864)
                     /locus_tag="PK198_03405"
     CDS             complement(10419..>10864)
                     /locus_tag="PK198_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF37812.1"
                     /translation="YRRILGVLLVFPVLRFFNVFPQAKERSLPLRWWMPPVLALVIGF
                     FSGLIGIGGGIILSPVLLMLGWASIRETAAISALFIFVNSIAGLAGAGVPDLALPAPL
                     WALMPMTIVAGGLGAYYGAHRFNVVTVRYLLAVVLAVASVKLMLG"
     assembly_gap    10865..10882
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10883..11156)
                     /locus_tag="PK198_03410"
     CDS             complement(<10883..11156)
                     /locus_tag="PK198_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRF37813.1"
                     /translation="MEQVWQLLFFLLPAAAFLYASVGHGGASSYLMFLALFNFSQAEA
                     RPTALILNIVVSLLAFLSHRRTCEFPTRLFLSFAIFSIPAAFIGGSI"
     gene            complement(11160..12116)
                     /gene="moaA"
                     /locus_tag="PK198_03415"
     CDS             complement(11160..12116)
                     /gene="moaA"
                     /locus_tag="PK198_03415"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRF37814.1"
                     /translation="MLFDTFGRIHNYLRISLTDSCNFRCHYCMPDENMACMSRAQLMQ
                     ADEIIRLAEIFTGLGVQKIRLTGGEPLVRVEFPDILRRLSKLPAELTISTNGALLHRH
                     IELLKDCGVRSLNISIDSLKPEVFHRITARDQFQIVWENIQLALQHGFGIKLNVVVMA
                     GVNDSEIMDFVEITRRQPLHVRFIEFMPFTGNNWQSGSVLTAAEMLERVGSELDVVKL
                     RDEPHATARKFKAVGYEGTFAFITTMSDQFCGECNRLRLTADGRIKNCLFGKEETDLL
                     SALRRGEPVLRKHAALGGQMSGDHQEVDASKIENRSMIKIGG"
     gene            complement(13014..14507)
                     /locus_tag="PK198_03420"
     CDS             complement(13014..14507)
                     /locus_tag="PK198_03420"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRF37815.1"
                     /translation="MTGNNEKTRVTIAYGDGIGPEIMAATIEVMNAAGAQLEYDEIKI
                     GQDVYLKGQESGMEPGAWEILRRNKIFLKAPITTPQGGGYKSLNVTVRTTLGLFANVR
                     PCRAYTPYVESYFPKMDLVVIRENEEDLYAGIEHQQTNEVVQCLKLVSRPGCEKIIRY
                     AFEYARAYGRKKVTCMSKDNIMKLSDGLFHKVFDEVAKEYPDIESNHFIIDIGAAMLA
                     DRPEMFDVIVTLNLYGDIISDITAQVAGSVGLGGSANVGENFAMFEAIHGSAPDIAGK
                     DLANPSGLLHAACLMLVHAGQPKVAEKIMNALLKTLEDGIHTGDIYSEKLTTQRAGTK
                     AFAQAVIERLGQAPQHLAAVQMGENAQPIEVKLTPSRAKAKRLLGVDIFLDWTGEHRD
                     PRELGQLLESLTGDQLQLSMITNRGVQVYPGGFPDAFCTDHWRCRFVARQSLHGTHRS
                     HNMAPDVIYYDIISLLQRLMSAGLNFIKLENLYEIDGKRAYSVAQGA"
     gene            14497..14766
                     /locus_tag="PK198_03425"
     CDS             14497..14766
                     /locus_tag="PK198_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37816.1"
                     /translation="MPVIVLLVDLADFMSVMVDERKMFVSGKTLCAPTAGKVPDFICP
                     GQRLSVCGRKKKCAERGIHPQNALQLGFRLRQMHALRQTPDQGIF"
     gene            complement(14774..15478)
                     /locus_tag="PK198_03430"
     CDS             complement(14774..15478)
                     /locus_tag="PK198_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF37817.1"
                     /translation="MSKPKIGVIIPAFNEEQAVGWVVQEIPRNIAAEIVVVNNNSTDS
                     TQEVAAAAGATVLSEKRQGYGWACMKAIEYFRDSAQPPDIVVFLDADHSDYPQEMPVL
                     LRPILEDGCDLVIGSRALGQRERGALTPQQIFGNWLATTLVRWFYGVRFTDLGPFRAM
                     RFEQLLALGMSEMTYGWTVEMQVKAAKQGMKCGEVPVNYRRRIGYSKVSGTVRGTIGA
                     GYKILATIFRLLVSSR"
     assembly_gap    15491..15500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15501..>16015)
                     /locus_tag="PK198_03435"
     CDS             complement(<15501..>16015)
                     /locus_tag="PK198_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF37818.1"
                     /translation="ERWIIHAVLAFAVVMTALVLYTYFTGSRNVLFFNSDAVRNWYGF
                     LIGSAFAGIVGTGFYPLMGNRVWCRFGCPLAAYLGLVQRFKSRFRITVNGGQCISCGN
                     CSTYCEMGIDVRHYAQRGQDIVRSSCVGCGVCAAVCPRGVLRLENSSADVQERASDVR
                     AIHIINGKVEL"
     assembly_gap    16016..16117
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16118..17269)
                     /locus_tag="PK198_03440"
     CDS             complement(<16118..17269)
                     /locus_tag="PK198_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37819.1"
                     /translation="MLFIGALLIFLSVNGLDRYRLTPESLGLSNVHHQQAILDAARQS
                     GMLGKTYASSFSFHSHLKKNIRAAQQTLKTTAQNGLPEGVNKGEYLLGDWIVKEYALQ
                     ITRQSAAGPVASHPLLFLMLTFGLGILGGLLYILPQFSGTAGIKNNHIYHRSMTRGLK
                     AGLRGIFLATTITGILIGGVAYMGGKWLWSLLALFVGGFILWRVFFTENPSTGCPARS
                     ASPEHTGWLGVLTGVLLIGFYILLYWAPQHITGWVRIVDPLSRALSGNPASHWFLYGT
                     LYTVVVLVMGVRMMAKYRHNKYQLVRTGSVMFFQTAFAFLIPEILVRLNQPYYDFKNI
                     WPLNYTFFFDYNLKTLTNSGGLGIFMLVWGIVLIVVGVPVITYFFGKRWY"
     assembly_gap    17287..17349
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17354..18763)
                     /locus_tag="PK198_03445"
     CDS             complement(17354..18763)
                     /locus_tag="PK198_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF37820.1"
                     /translation="MQQHIVIIGNGIAGITAARQIRKLSEHRITVVSSETDHFFSRTA
                     LMYVYMGHLRFEDTKPYEDWFWKKNRIELLRGFVKKVEPEQRRLLLEDGSTLCFDKLL
                     IATGSRSNKFGWPGQDLAGVQGLYHYQDLESMERHSAGLRRAVIVGGGLIGIEMAEML
                     HSRHIPVTFLVREKNFWDGVLPLEEAQMINRHIRENGIDLRLETELKEILPDAQGKVR
                     AVVTNKGETIECGFVGLTVGVSPNVAFLQDSGIETDRGVLVNEHLETNIPGIFAAGDC
                     AQLRAPRPGRRPIEAVWYTGRMMGETAAYNLTGGDQSAVGSGQWAVGGERLAVGAGLV
                     PYDPGIWFNSAKFFDMEYQVYGTILPNMPEHQSAVYWEHPEGRRSVRIAYQRQTGNVV
                     GFNLMGVRYRHEVCEKWLRAGTHIEEVLQNLGIANFDPEFYRQYETEVIAQYNRQSGR
                     NLRLKKRRGLAGALAFLRG"
     gene            18970..>19197
                     /locus_tag="PK198_03450"
     CDS             18970..>19197
                     /locus_tag="PK198_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37821.1"
                     /translation="MKKKTTSQLARVYVSLSLVFASAGALFSQQALPPPPPPDCGGFE
                     LADLWPSLRYGRIISGIITDLENKSLIGVNIS"
     assembly_gap    19198..19207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19208..19780
                     /locus_tag="PK198_03455"
     CDS             <19208..19780
                     /locus_tag="PK198_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37822.1"
                     /translation="DASGMYMPYGTVSDMDGAFQLMVPVTDDDLYLTFSYVGYEKKSI
                     KLKKVHPQLNIRLESIAYELSEVVVIGYRTTMQKSINIDYSFPSSQKEQLFVPAAACD
                     LSVGQVYPNPFTTELHVRLEGRQAGAAQLHLFDAAGRSVLSRTEILQEGMQTVSFNLN
                     DYRLPGGVYYLRVSDAAGEISTHPVVRVDD"
     gene            complement(20312..20743)
                     /locus_tag="PK198_03460"
     CDS             complement(20312..20743)
                     /locus_tag="PK198_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37823.1"
                     /translation="MWWILTALIAAALLLPIRYWAPEFPFFGINLIFIVVFITLSRYI
                     FLLPHTWLAGKGRIKIVLLFLCMPLVFLLIQELNHFQTFLDENGPETLVGPLSYEMTQ
                     SMIGYVRGEMLLFAVGSIVAGVLFPIRMVVSVWRTRNLGRE"
     gene            20763..21110
                     /locus_tag="PK198_03465"
     CDS             20763..21110
                     /locus_tag="PK198_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37824.1"
                     /translation="MRILSDPIVRFRFDTRNTTPAESGGSMSACREVVVDRLKTLIKI
                     KNPADNYQCAVFKRKATFGKKKRFSPLQINEYEPIATQLNNLKNTGNESGKTYPRHHD
                     NGPDNRFSRRKYP"
     gene            21064..21486
                     /locus_tag="PK198_03470"
     CDS             21064..21486
                     /locus_tag="PK198_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF37825.1"
                     /translation="MITDLITVSPDASIREIQYLFEANGFHHLPVMDKGEMLCGIISR
                     EDFFKLAYELSLKTTGKTWTEREYNAMKAKDIMTKNPLVLDADDSIGLAADIFLANRF
                     HALPIVEENRLVGMVTTHDLLLYSFNSSFIEKETGDIL"
     gene            21483..21914
                     /locus_tag="PK198_03475"
     CDS             21483..21914
                     /locus_tag="PK198_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF37826.1"
                     /translation="MMTIDKTTTVAEIMTERVIVVRPNETMDVVQDIFRKNNIHHVPV
                     VDKGMVVGMVSHADYLKLLHGFTLFKTKKSDEYNDAILRSLLVKEVMTKQVATLGPED
                     SLELAAGFFRENLFHALPVVDEENMLIGIVTTYDLLNYAYG"
     gene            complement(21905..>22125)
                     /locus_tag="PK198_03480"
     CDS             complement(21905..>22125)
                     /locus_tag="PK198_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF37827.1"
                     /translation="AAALDEEMTRQNIYYKDLIAGNILRPCVVRPLPHDTFRQYMKSL
                     GKLGGQNKVPRLSNDRKIADALQSFLQP"
BASE COUNT         5183 a         5735 c         5648 g         5193 t
ORIGIN      
        1 tgtcattgtc taaccagttc aaatacagac cattgactac tttttcgttg atttcaaagg
       61 aatgtgttgc actgatgctg tcattttggg agtacagctt accggcttcc gatttcacat
      121 tgctccatat accctccgac aagtctttat tgtcggacga caattcaaat aaatcaccga
      181 gcaattgaat tttaaccggc ataagcgccg ctatacctgc ggaatcggct tccagcatct
      241 gttcgtcaat gtgaacaagg ctgtctaatt tctgctctac aagagccaga ttgtacttca
      301 actgattcct ttccaagcta tcggccgaaa acatagcatt gataccgtcc tgtatggcgt
      361 aaaatgcgcc gatgctgttc gtgtcgcttc gggcaaggaa ttcaaatatg gtcgggttgg
      421 ataccgaggc accctgcaaa cgctcataga catagcgctg tagttcccac agcacggctg
      481 cgcttatcaa agtatctccc aaagctattt tctttacgtc atcggattcg ggcgcaaaac
      541 cgttcaccat acagacctct tcatcgcaag ggtccatatc ttccgtatct gtggcagatt
      601 gaaaccaatc ttcaggtgag aaatcttccg gctcataaga agggtctccg ttggatgacg
      661 atacatcaac aaagaagggg aggttttcag caaaatttga cgacacctca tataccgccg
      721 gcccggcatc ctgtagccac ctgttcttcg tatgcgtctg cgtacccaaa atggcattca
      781 catcgggcag cagcaggctg gtagcgtgat tactaaagga ggtaccccgg aaattgtcgg
      841 tagcgaggct gccgccgctg atgtgggcgc ctatgcgggt attgctcagg gtgttgcagc
      901 aatacacatt gccggcggag gagactatgc gaagggcaat gttgtcgggg ccgccggtgc
      961 cgccgcccgt tacggtgttg tcttccagca gtgattttgt aacgttggcc agatcaatac
     1021 cggccgcagc ggctgtcggg tcggacaggc tcacggtgtt gtcccgaagg gttgattttg
     1081 cggctgccgc gctcccaatg cccataccgg ctttgcctac cgtcacggtg ttgttgtcta
     1141 acaaagcggg cgccgagctg aacaattgcc agatgccggc aatggcattt tcggtgtcgg
     1201 tattcactat gttgtcggtg atgacggcgc cgccattggg atcaggatgc gcaaacacaa
     1261 tgcnnnnnnn nnngaggcga gtggcttcta tttcgttgtt ggtgatgctg atgccgccag
     1321 cgctgccaat ggctgcccaa atgccgactt ccatttgcag catctcattt tccgttgctt
     1381 tcagcttggc gttgttggcc atgatgccct gcgggcaatc ttcaaagtag cccttttcca
     1441 cctttgccga atgtactccg gttgactgaa tgctcactcc tctgccccgg taatcccagg
     1501 cgggataggc cgcgttgttg ctgcggatgg aaatttgttg atccatgtac gaacccagat
     1561 tggaatgtcg ggccactatg ccggaagtaa gttgctcgaa gcgattattg aggctgttga
     1621 aattggccag attatcagct acaatgcctg ccagcgcgtg ttggttttgc tcgggcagcc
     1681 cctccggcgc gctgctctgc ccggagaagg gaggaagcag gcttgcggcg gtttgcgtgt
     1741 agcgattact gcccactgct gcattcacgc ttccgggcga tgcgcctcca gccagcaccc
     1801 ctacaaagtt gttgtcaaaa tcgttgtcat aaatgctcac tccgcccgtc agaggatcat
     1861 ttccggggta ggagtgcaca aacacggcat actgcgcatc ggagacacgg tttcctatca
     1921 ttttcatgcc gccgccctgc tgcacttcta tggcatacca catgttgtca caacctgaaa
     1981 agtctgaatc aataatggct aatgtactgc catcctcaat ctcaatacgg gaaccgggca
     2041 acatataaaa aatactgttg atgatgttgt agttttggtc tatcaacagg gttcctttta
     2101 cgcacacctg aatatctgta ccatcagccg gcaacaaatc attcccgatg gctacggaaa
     2161 gtttgagcgt ctggccgtcc tctccgatga gagattcctc gcaggtttcg caactgaatg
     2221 ggggacaata atcggtttct gcgatagcat tcagattggt ttctccgcac acattcgaaa
     2281 tgactacatt nnnnnnnnnn nnnncatttt cataagcatg aacgatcgcc gtgccgctgc
     2341 tgccgtctcc gtagttgatc aggtagctgt cgccggggtg caggttattc aggaaaaaag
     2401 ttacctctcc acaggactct gaaagcactc cgaactccgg cataggagga gaaggcaaaa
     2461 ccgttactgt aacatcgtcc tccaacgtac aaaactcgaa tgaggaaggc agattatgtg
     2521 gagtagtgag tgaacggcgt acccggtatg tagtggtttg ttccggctca ataaaaagtt
     2581 gttgtgtttg gtgatctaca tctccgttga ctactgtggc cactccgttc agtactgtgt
     2641 attcagctat gagcgtttcg ttcacacctg ttacttcaaa ccattgataa cgcaccggca
     2701 catcggagag catacagaac gggccgccaa gaaagtcgca tcctgttgaa acaaaatcct
     2761 cgccggcgct gaagtcgtcg gggataagtt caacttcgtc aagaaaaaca tccagacgct
     2821 gagccccttc atcagggtaa aaccaaagtg aggagtagga ttctgcaagc tcaacacata
     2881 atgctgcacg gctaaaagaa ccagagttaa gatttgcatt tgagccgtaa aaagggaacg
     2941 tatggcttgt aagtaaagaa taattgcccc cgttcaagtc aacttgggat ggacgggacg
     3001 gaaagttggc gcctctcccg aaaagggcag taatgtcagc atttacaagc attgcattca
     3061 ttaccatgtt tccagttaac atcgtgctac tgtgcttggc agtagtataa aaggagaaga
     3121 aatagttgcc ttgcattgca ttggcaggtg taaatgcaga ctcaatcgta cttccgaaac
     3181 cgccacgtat catgtgaaca tttcctgaac cagtaaacgc ggtgggattg gagtaataat
     3241 caattgtacc tgtagctcca aaccagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 cagattccaa ctcaagaaca tcaccccctg ttgtaccgtg tactactatt tcggggttac
     3361 aaatcaagtt gcatgtgtta ttgttgtggc ctccattgat cggattatct gacaaaaaga
     3421 aagagcaatc ttcaaaacaa cgccttacca gtatttcgac aaaagcggta ctgccatcct
     3481 ggcaatcttc tgcaattggc gtgtatttga atctgtgaac tcccggtgcg catggtgtat
     3541 atagtatgga gcctcctacg acttcaaggg tacctatatt agggtttatg ttgaccaaat
     3601 ttccaatttc ttgattcggt tgatcgtttt ccagaagcgc tgaaagattt atagtaatat
     3661 ttagtgcatc ttcacaaagc tccagtacaa gaggttcgcc atcacagcca tgtcttaaat
     3721 catggtcgcc ggctgctacg cacgaacaat tttccatttg tgtaacctca tagcccagag
     3781 cgcaaagtat cttcaactct gcttcaccga tatcaactct gacacctcga tcaaaagatg
     3841 gcttcattaa gtaatcagcc cccgcatttg gacaccttgc atccagatgg ctgaacgcag
     3901 cgttatccat ctcattatat ataggcagag catctccggc cataggtgga aataccatat
     3961 tttctgtact gttgcccaag cagctggtat gtaagtctcc ctgattgata tttaaattcc
     4021 acacataagg atcattggca tcttgaacta ctactttagc cccattttgt aacttcaaaa
     4081 aagtatcata acggttgtaa gaggtaaacg atgttgaaaa gtaaccaccc gtacttggat
     4141 taagtaacga aaaaaagcct acggcgtgca gcgcctcatg cagcaataca tcatacagat
     4201 caaacaagtt accagctgat gcatccggag gcactacttc ggtatagaat gattttgtaa
     4261 aatcaacacc tatgcttgca tggaatatgt cggcattacc cgataatgaa gtatagcgac
     4321 cattattaag aattttccaa ggtaaacaat caatgatacc cccttccaga gtataattca
     4381 gggattcgaa cacaggactg gctgcagcca gcactgggcc gttttgccac gagactactt
     4441 gagaattgtc ataaggtaaa aatctgatgt tcactttact aaaatcatca ccttgacaag
     4501 gagaggttcg ctgtatgata agttgagaga gatttgcaaa tacccggcac gctacttttc
     4561 tccgcgcaat tccaaggtct cccaattgct gattgaaccc ggcattattg ttatagacac
     4621 aatcttcaaa aaccagatta aaatagcttg cactcaagcc tggaatatct gcgtccgtaa
     4681 actccgcaca gccacaccct gaattcagat tttccggcag cagtagttct tcttgttcat
     4741 ataaatttcc gaatcggtcg gcgtagtagg ttttggtaga atctatctct ggaatatctt
     4801 taacgaaagc tccgcacagt cccggcggta gttcttcccc tactccctct atctcaatcg
     4861 tcaacgtagc cggaccttct atttccgtgt tgttgcctcc ttgaattcca ctcagttcaa
     4921 atgaataaac gcctacagta tccgaggctg cagcaaccag ataaaaacat tggagcgtat
     4981 cgcttccgct gaaagtgagc gtcaccggtt cgtaaccttc aaagtgaggg agcgaatcgg
     5041 agaccaatgc tatatccacg cttacagggc tgcatacaca agggttttgc agcgttgcgc
     5101 atatcagtat cgaatcgccg ctttgagcct gaatcaggct atccgcaaat gctatggagg
     5161 tagccggagt tgcgtccgac tgtgtcacgg tcacattggt gaaaacagcc tgcacattgt
     5221 tgttactatt actgctgtaa gcgtacatcc ccagtaagac acaattgtcg gcggtagcaa
     5281 acggagtcgt aaaagctaag ttccatgtgc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacttgc attgactgca gcctgttgtt
     5401 gaaacattcc ggtacttgct ctcaactggc ggaatacctg tgcgcctttt tgagtcataa
     5461 cagcgcattt cctggagccg acagcactgc tttctctcac aaaaagtccc gcagcacctc
     5521 ccgacaacga tggaatttta acagtcagcg aaccattgcc gcatagctca tggtaaacca
     5581 tttcgtggtt atcagaggtt gccatcgccg gggtaaccgc gtttgtcgtc agtacaacag
     5641 tatccggcat gcaggcagca tacgcagaac tgccggtgcc gctgcccagc gacatatcct
     5701 gccaggcatc gcaaaatacg ttggaactac ccgttgcggc cgataaagct acatggctca
     5761 tggcatcaac gtgtacacct tgttcagtgt gtgtgtgtgt gtgtgtgtaa acactccctc
     5821 gaaaacggac gagggtacaa atcgcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnccaacagc atggcggtgt aaaaaagggt ggcttttttc ataatgcaaa
     5941 tgtatttaag ggttaaaaaa tgcgtgatta tgaaggggaa cgcggctttt ccgcagggta
     6001 aaacttaata acacaggcaa atgtaatgac tgggacggga aagaaaaaat atatggcggt
     6061 ctttttctgt gtgctgttga aaatccggca gataccgttc atctgcactg tgaaccgtgc
     6121 ctgtttttgg cacaatttgt cactaaagat acccgtttgc cggaaattgt atcatttacc
     6181 attctattcg caccgaagac tgcctactgc aaaatccgac ttccccccag tttttttcaa
     6241 caatttaatc tgctcgatgg tgatgtgttt gtcaatcggt ttgagcatgt cgtacatggt
     6301 gagcgccgcc acggaggccg cgtgcatggc ttctacctcc acgccggttt tgtaaatggt
     6361 gtgaatttcc gcgatgatga ggatgtgatc gggctgaata tcaaactgaa aagcagcgta
     6421 ttccaccggc agcgggtggc agtcgggaat catatcgctg gtgcgcttgg cagcaaaaag
     6481 accggcagct ttggccatct cgcagacgtc gcccttgggc acccgttttt cgagtacggc
     6541 ctgtatggtt tccggcgaac cggcgcgcac aatggcctgt gcaatggcgg tgcgcagcgt
     6601 atgatttttg tgtgtgatgt tgaccattct ttattcatat ttatttagtg cgtcccatcg
     6661 ccattagcaa atagctactg gctgttagcc tggttttccc aaaagaaaaa gaattcattt
     6721 tcaaaagtac atttagaaat ttccccatga ggaaaatctt ttcccctaag aaaaaacgac
     6781 cacgccctgc gtggtaatag ccagcagctt gcagcctttg ggatgccaat agtaacgttt
     6841 tactcaggta ttgactttcc aaaaagagac atcctgatcg gtgagttcct gtccccacac
     6901 gggcagttct tttttcaggc gttctacggc agcagcgcaa gcctctatgg ccgggcgccg
     6961 atgccgggag caggccagca ccaagaggct gatctcaccc gttttcaccg tgccgagact
     7021 gtggtaaatt tcgagcagct cgagttgata cgcctccaca acggcggcgc atatttcggc
     7081 ggctttttcg cgggccatgt cttcgtaggc cgtgtaatga atggccgtca cagggccggc
     7141 gggcgtttcg tcggcgcgca cctgccccaa aaaaatactg tgcgcaccga ggccggtttt
     7201 actttgcagc cactcaacgg cttgcgcgag cagcgccgaa gtgatggcgc catgtacaaa
     7261 taaattatcc atgcgtaacg ctgtgatgta tgggcgagag cgggtgctga agccctccct
     7321 cgatggaata aacctgtgta tggcggccgc ttttttgcaa taccccggcc aggtgccgac
     7381 tgcgcacgcc cgacctgcaa aacaacaaca gcggcacgtc ggtggtcagt tcggaggcgt
     7441 cctgcaacaa gcggctcatg ggccaccggc ggctgcgtac atgcacattg gggccttctt
     7501 cctcctcccg aatatcgagc agcagggttt cagggcggcg ttccaacagg gcctgcgcct
     7561 ccgtccacaa aatatcctgc acgcgctcgc aggcatcggc gtagtgcatg gccctgaacg
     7621 cggcttcgtt agcgggcatc atggcctgcg catcggggtg cggagcaatt tccacctcaa
     7681 aaaaagaggc gtttttgaaa tggtaaaaca gggccttgtc gagcagcggc tgccctactc
     7741 ctgtgatgca cttgatggtt tcggcggcct gcatcagccc gatgataccg ggtagcaccc
     7801 ccaatacgcc ggtttcgctg caattgggcg cgtcgccggc ggccggcggc acggggtaga
     7861 ggtcgcggta agtgctgcgg ccccgttgcg catcgaacac agccacctgc ccttcgtatt
     7921 gataaacagc gccctgaacg agcggttttt ggagcaaata acaggcgtcg ttgaccatgt
     7981 agcgggtcgg gaaattgtcg gagccgtcca ccaccacatc gaagcgggcg atggtttcca
     8041 atgcgttttc cacatccata aaaaagggca gcgcctcgaa ttccgtattg ggaaactgct
     8101 cccggagccg tcgggcggcg atttcggctt tgggcaaacc aatgtcgtcg gggccataca
     8161 acacctgcct gttgaggttc gattccgaca ccgtatcgcc gtcaacgatg ccgatgcccc
     8221 ctacgccggc agccgccaga tagagcaaca ccgggcagcc caagcctccg gcgcccacaa
     8281 ccaaaacgcg ggcgcggctc agcttttcct gtccggcagc gccgaaatcg ggtagcagga
     8341 tttgtcgttg aaatgctgac atcggtatcg gatgattgaa tgtcggtgca aggtacgact
     8401 attttggttt cgggtttcat ccgctgaacc acgccacagg cctgggaagg tgggtttcgg
     8461 gttcgtgctt ccggggttcc atcgcgcttg gcgaccggcg cggctaccta agacggcaac
     8521 atccgcctga tctccctcgc ttgttccaag tggcgttgtt catgcgcgac gatgatgtca
     8581 aaagcagttt ctaaagtata gacgatgttt ctgttggccg gagaagagat aacggcgcct
     8641 ttttggatga ggtctgagga gtcttcgagg agttttttca aatcctgttg atgcttgctg
     8701 aagcgttcca gaatttcggc gccgatttca ctttgagcag gttcccagat cggaaaggta
     8761 ttcatttttt tttcgccggt cgggttggac ggcgtttaaa acggttttcc ccaaaaaaga
     8821 aaccatgaac cccaaccttc ccagaaaggg cgttctgtaa gttcctgctt tcagtgacgc
     8881 aatgaccgga aaataggttt cgttgacgac gatgaggtgc tcgatgtttt gggcgatgct
     8941 ccagatgccg ggcgaaggtt tccagttcag ttgcccggcg tcaagggaag caaattcctg
     9001 tttgacggca tgggttactc cgtcaatttc agacatccat ttcattgggt atgttctttt
     9061 ttaattccca acaaatactg caccggcaga gcgcccgccg ttttgccgat ttcccattca
     9121 aacgcatcgg aatggcgcag cgaggcgact aattccttca tctctttttc agaatacgtt
     9181 ctcaaaatag atgcgatgcc atcccacaaa acgtacagcg gcaccagtgg taaaaggtag
     9241 ataaacggca gtactttcca acgcaggggt ctgataaagg gtgtgaaaag cagcacattg
     9301 agcataacga aaagcataga gaaccaactg atggcatttc tgcccaccgg ctcgaaaatg
     9361 gcgatgggcg actgcgtgtc cacggcatct tgcaaaattt tacgggcatc gtcgggcctg
     9421 aaatgatgaa aagcgccgaa aagggtgatg aaccttccgt gcaaatgtgg gggcatctcc
     9481 atcgcgttga ccggctgttt ttcaaaatca aatacatttt tcagttccgc ctgtgtgcgt
     9541 tcaaaggcgc ggatattggg gtagtaatcc gtcaacgtga ttttgaggtc gggaaattcc
     9601 gaatgcaagg ctttcgccaa cataatcagg ctgctgcctc cccccgatgc gcagtccacc
     9661 caatgccggc cgccgctcgc ttggagtcct tttcggatga gcggcacaat gggtttgtac
     9721 agatcggatt tggtggctac gaaacgcagg taatccgtgc cgtaatctct ccagcttgcg
     9781 ggaaaccatc tgaggtcttc ccactccaat gcgtgtattc ttctcatgtt gcctcagcgt
     9841 gaatgaattg caaaggtatg gttgattggg aaatgggcat agacaaaaat ccccctctat
     9901 ccccccgaaa acggcggcag cagcgccacc tcgctgtcgg cttgcagggg cgtattttcg
     9961 tgtactattt ttcggttgac ggcaatggca aatcggacct ctcgcagtgc cggatgctgt
    10021 tggtacaaag cttccagcaa ttgctccgtg tcggagacgc cggagatact cagccgggaa
    10081 ctgccggctt tctccgccac aatgccgaag gcgaggagct gtatggttgg ctgggttgtc
    10141 atggttttta caatgagtcc tgtttttatt ttaaaaaatc gcacctattt agctggatgt
    10201 tttcaggtga catctaaccg ccgaacctta gcgaggcggg aaggtgtcgc atgaaaacag
    10261 gggagatttt cccttcacag ccgcgagatt acaccttccg ccctccggtt gttacaacag
    10321 ggcggcagat gtcacctcgc taatgtttca tcctaagcag ttacaaaaaa tcaaacttgc
    10381 agcttgcggc ttgaggcttg cagccggcaa tcggcagcct accccaacat caacttcacc
    10441 gaagccaccg ccaacactac cgccaacaag tagcgcaccg tgacgacatt gaaccgatgc
    10501 gcgccgtaat aagcgcccaa tccgcccgct acgatggtca tcggcatcaa agcccagagc
    10561 ggagccggca aagccaaatc gggtacgccc gcgcccgcca atcctgcaat ggaattgaca
    10621 aaaataaaca aggcgctgat ggccgccgtc tcgcggatgg aggcccaacc caacatgagc
    10681 agcacgggcg acagaataat gccgccgcca atgccgatca gccccgaaaa aaagccgatg
    10741 acgagggcca ataccggcgg catccaccag cggaggggca gactgcgctc tttggcctgc
    10801 gggaatacat tgaaaaaacg cagcaccgga aaaacgagta ggacgcccaa aatgcgacga
    10861 taccnnnnnn nnnnnnnnnn nncgatggag ccgcctatga aggccgccgg aatggaaaag
    10921 atggcaaacg acaaaaacaa ccgcgtggga aactcgcagg tgcggcgatg cgacaaaaac
    10981 gccagcaacg acactacaat attgagaatc aaagcagtag ggcgagcctc ggcttgtgaa
    11041 aaattgaaca atgccagaaa catgaggtag ctgctggcgc cgccgtgtcc caccgatgcg
    11101 tagaggaacg ccgccgccgg gagcaggaaa aacaacagtt gccagacctg ttccatcgct
    11161 tagcctccta tttttatcat gctgcgattc tcgatcttcg aagcgtccac ttcctgatga
    11221 tcgcccgaca tctggccgcc gagtgccgcg tgtttgcgca gcaccggttc gccccgacgc
    11281 agagccgaga gcaggtcggt ttcttctttt ccaaacaggc agtttttgat cctgccgtcg
    11341 gcggtgaggc gcaggcggtt gcactcgccg caaaactggt cgctcatcgt ggtgatgaat
    11401 gcaaaagtgc cttcgtatcc gactgccttg aattttcggg cggtagcgtg cggttcgtcg
    11461 cgcaatttca ccacatccaa ttcgcttccc acccgctcca gcatctcggc ggcggtgagc
    11521 acgctgcccg attgccagtt gtttcctgtg aaaggcatga attcgatgaa acgcacatgc
    11581 agaggctgcc ggcgggtgat ctccacgaag tccatgattt cgctgtcgtt cactccggcc
    11641 atgaccacca cattgagctt gatgccgaat ccgtgttgca acgctaattg gatattctcc
    11701 cacacgatct ggaattgatc gcgggcggtg atgcgatgga aaacttcggg tttcagcgaa
    11761 tctatgctga tgttgaggga gcgcacgccg cagtctttca gtagctcaat gtgccggtgc
    11821 agcagcgcgc cgttggtgct gatggtcagt tcggcgggta gtttggagag ccgccggagg
    11881 atgtccggaa attcgacccg caccaagggt tcgccgccgg tgagcctgat tttctgaacg
    11941 cctagtccgg tgaagatttc cgccagacgg atgatctcat cggcttgcat gagttgcgcc
    12001 cgcgacatac aggccatgtt ttcgtcgggc atgcagtaat ggcaacggaa attgcagcta
    12061 tcggtgagcg atatgcgcag gtagttgtgt attctgccaa aggtgtcgaa cagcataagt
    12121 gcaaggcgtt gatgctctga tgcctgctga taccgaaaca gacatcaaat gaacaaatca
    12181 acatatttgc cgacgccttg tttcgcgcag gcgaagatac gccggaatat tttggtatct
    12241 gctatctgaa tgcccgcgct caggatgccg ggcaagcgct gctaagtcat ggctctgcat
    12301 ctaactcaca cacctcactg cccgataccg cgccattcag gcagcattgg ccgtagctgc
    12361 ggtgttctgc gattttgatt atttgggatg cgcccggtat ataatgcccg ctgaaaatct
    12421 tgttgggtta aaaagtggtt cattcaaact tctgattggg aaagtatgct tcagttcaat
    12481 atccgtattt ttaaaaagct cgtatgaggc tgttactcct atgcctttta cctgatagtt
    12541 aaccaaccca tctgtattat atggagatgg gatgaccaaa tagcgcgttt tgcctgctgg
    12601 cgcgagattc catctcgtgc cgctatccgg cgggttctac tcgccaaata ctcgcttttg
    12661 caactgcttt acacgcgggt agaacccgca aaatacccac gcgagatggc aggactgcaa
    12721 ccggcagtgc gccgggtgtc atctggcgca gcgtggaatc tgcatttgcg aaacttccaa
    12781 aagtttcgta aacgtaacac actggttatc aatattattt ttcgggctta gtgaaggggt
    12841 gatttgcctt gcgacggcac agattatcac cccttcacta cacatgtacg gcctactacg
    12901 attacgaatc ctgaaaggct tcgcaatcgc atcgcttggc tgccggttac aattacgaaa
    12961 cttcgaaagt ttcgcaatcg cttggccgat gaactgcgca gcagcttgct ggattacgcc
    13021 ccctgcgcca cagagtacgc ccgcttgccg tctatctcgt acaggttttc caacttgatg
    13081 aaattcagcc ccgccgacat gaggcgttgc agcagggaaa tgatgtcgta ataaatcacg
    13141 tcgggcgcca tgttgtggct gcggtgcgtg ccgtgcaacg actgccgcgc tacgaaacgg
    13201 cagcgccagt gatcggtgca aaaggcatcg gggaaaccgc cgggatacac ctgcacgccc
    13261 cgattggtaa tcatgctcag ttggagttgg tcgccggtca gcgattccag caattggccc
    13321 agttcgcggg ggtctctgtg ctcgccggtc cagtctaaga aaatgtccac acccagaagg
    13381 cgcttggctt tggctctgga aggcgtcagt ttcacctcaa tgggctgggc attttcgccc
    13441 atttgtacgg cggccaagtg ttgcggcgcc tgtcccaatc gttcaatgac ggcctgggca
    13501 aaggctttgg tgccggctct ttgggtagtg agtttttcgc tgtaaatatc gccggtgtga
    13561 ataccgtctt cgagggtttt gagcagcgcg ttcataattt tttcggctac tttgggttgc
    13621 ccggcgtgta ccagcatgag gcaggccgcg tgcagcagcc ccgatgggtt ggccaagtcc
    13681 ttgccggcaa tatcgggcgc ggaaccgtga atggcctcaa acatggcaaa gttctcgcct
    13741 acgttggcag agccgcccaa gcctaccgag ccggccacct gcgccgtgat gtcggaaatg
    13801 atgtcgccgt aaagattgag cgtcacgatg acgtcgaaca tttcggggcg gtcggccagc
    13861 atggcggcgc cgatgtcaat gatgaagtgg ttgctttcga tgtcggggta ttctttggcc
    13921 acctcgtcga aaaccttgtg aaacaagccg tcggagagct tcatgatgtt gtctttcgac
    13981 atgcaggtca cctttttgcg gccataagca cgggcgtact caaacgcata gcggatgatt
    14041 ttttcgcagc cgggccgcga cacgagcttg aggcattgca ccacctcgtt agtttgctgg
    14101 tgttcgatac ctgcgtagag gtcttcttca ttttcgcgga tgaccacgag gtccattttc
    14161 ggaaaatagc tctccacata cggcgtgtag gcgcggcagg ggcgcacatt ggcaaagagg
    14221 cccagggtgg tgcgcaccgt cacgttgagg cttttgtagc cgccgccctg cggggtggtg
    14281 atgggcgctt tgaggaagat tttattgcgc cgcagaatct cccaggcgcc gggttccatg
    14341 cccgattcct ggcctttgag ataaacatcc tgcccgattt tgatctcgtc gtattccaat
    14401 tgagcgcccg cagcgttcat cacttctatg gtggcggcca tgatttcggg gccgatgccg
    14461 tcgccgtacg cgatggtaac acgcgttttt tcgttattgc cggtcattgt tttgttggtt
    14521 gatttggcgg atttcatgtc cgttatggtt gatgaaagga aaatgttcgt ctcagggaaa
    14581 acgctgtgcg ctcccacggc gggcaaagtt cctgatttta tttgccccgg ccaacgactt
    14641 tcggtatgcg ggcgcaaaaa aaaatgtgca gagcgcggta tccatcctca aaacgcacta
    14701 caacttggtt ttaggcttcg gcaaatgcat gccttgcggc aaacgccgga tcagggcatt
    14761 ttctgacgac gagctaccgg gaactcacca acaacctgaa tatcgtggcc aaaattttgt
    14821 accccgcgcc gatggtaccg cgtaccgtgc cggaaacttt ggaatatccg atgcgccggc
    14881 gatagttgac gggcacctcg ccgcatttca tgccctgctt ggcggccttc acctgcattt
    14941 ctaccgtcca gccataggtc atttcggaca tgcccaaggc caacaactgc tcgaaacgca
    15001 tggcccggaa cggccccaga tcggtgaaac ggacaccata aaaccagcgc accaaagtag
    15061 tagccaacca gttgccgaaa atctgctggg gcgtcagcgc gcctctttcg cgctgcccca
    15121 aagcccgcga gccgatgacg agatcgcagc cgtcttccag gatcgggcgc agcagcaccg
    15181 gcatttcctg agggtagtcg gagtgatctg catcgagaaa aacaacgatg tccggcggct
    15241 gagccgagtc gcgaaaatac tcgatggctt tcatgcaggc ccagccatag ccttgtcttt
    15301 tttctgacaa aaccgtagcg ccggctgcgg cggctacctc ttgggtgctg tcggtagagt
    15361 tgttgttcac cactactatt tcggcggcga tattcctcgg tatttcctgc accacccatc
    15421 ctaccgcctg ttcttcattg aaagcgggaa tgatgacgcc tattttgggt tttgacatga
    15481 tagagatgtg nnnnnnnnnn tataattcca ccttcccatt aatgatgtgt atggctcgta
    15541 catcgcttgc ccgctcctgc acatcggcgg aactgttttc caaacgcagc acgcctctgg
    15601 ggcacactgc cgcgcaaacg ccgcagccca cacaactgga gcgcacgatg tcttgcccgc
    15661 gctgggcgta gtggcgcacg tctatgccca tttcgcaata ggtggagcag ttgccgcaac
    15721 tgatgcactg cccgccgttg acggtaatgc gaaaacgact tttgaagcgc tgcaccaagc
    15781 cgaggtatgc cgccagaggg cacccgaaac gacaccagac ccggttgccc atcagcgggt
    15841 aaaaccctgt acctacaatg cccgcaaaag cagagccgat gagaaagccg taccagttgc
    15901 gcaccgcatc gctgttgaaa aacagcacgt ttctggagcc ggtgaaatag gtgtagagta
    15961 ccagcgccgt catcactacg gcaaaagcca gcaccgcgtg gatgatccag cgctcnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta ccagcgtttg ccgaaaaagt
    16141 aagtaatgac cggcacgccc actacgatga gcacaatgcc ccacacgagc ataaagatgc
    16201 cgagaccacc actatttgtc agggttttga ggttgtaatc aaaaaagaaa gtgtagttca
    16261 aaggccagat gttcttgaag tcataatacg gctgattcag ccgcaccaaa atttccggaa
    16321 tcaggaaagc gaaggccgtt tgaaaaaaca tgaccgaacc ggtgcgtacc aactggtatt
    16381 tgttgtgccg gtacttggcc atcatgcgga cgcccatcac caagaccacc accgtgtaga
    16441 gcgtcccgta caggaaccag tggctcgccg ggttgccgct caatgcgcga ctgaggggat
    16501 caacgatgcg cacccagccc gtgatgtgct gcggcgccca atacaagaga atgtaaaatc
    16561 caatgagcaa tacgcccgtg agtacgccga gccaacccgt atgttcgggc gaggcagagc
    16621 gggccgggca ccccgtggag ggattttctg taaaaaacac acgccataaa atgaagccgc
    16681 cgacgaacaa ggccaacaaa gaccacagcc acttgccccc catatacgcc acgccgccga
    16741 tcaggatacc ggtgatggtg gtagcgagaa aaattccgcg cagccccgcc tttaagccgc
    16801 gggtcatgga gcgatgatag atgtggttgt ttttgatgcc cgccgtgccg ctgaattgag
    16861 gaaggatgta cagcaagccg ccgagaatgc ccagcccgaa ggtgagcatc aggaacaaca
    16921 gaggatggct tgccaccggc cccgcagccg attgccgggt gatttgcagc gcatactctt
    16981 tgacgatcca atcgccgagg agatattccc ccttgttcac gccttcgggc aggccattct
    17041 gggccgtagt tttgagcgtc tgttgggcgg cgcgtatatt ttttttcaga tggctgtgaa
    17101 aggaaaaact cgatgcgtag gtcttgccca gcatgcccga ttggcgggcg gcatccagaa
    17161 tggcttgttg atgatgtaca ttgctcaggc cgagactttc cggtgtgagc cggtagcgat
    17221 ccaaaccatt gaccgacagg aaaataagca acgctcctat aaagagcaca agccctgttt
    17281 gttgtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnt ccttcatcct cgcaaaaacg ccaatgcgcc tgccaggccc cgccgctttt
    17401 tcagcctcag attgcggccg ctttggcggt tgtattgcgc tatcacctct gtttcgtatt
    17461 gccggtaaaa ctccgggtcg aaattggcga tgcccagatt ttgcagcacc tcctctatat
    17521 gcgtaccggc gcgcagccat ttttcgcaaa cctcgtgacg atacctcacg cccatcagat
    17581 tgaagccaac gacgttgccc gtttgccgct gatatgcaat gcgaacactg cgccgcccct
    17641 ccgggtgttc ccaatacaca gcgctttgat gctctggcat attgggaagt atcgtgccat
    17701 atacctgata ctccatatcg aaaaacttgg cgctgttgaa ccatatcccc ggatcgtaag
    17761 gcaccagtcc cgcgcccacc gccaaccgct caccgcccac tgcccactgc ccactgccta
    17821 ccgccgactg atcacctccc gtcaaattat acgctgccgt ttcgcccatc atgcgcccgg
    17881 tgtaccacac ggcttcaatg ggccgcctgc ccggccgggg tgcgcggagc tgagcgcagt
    17941 cgcctgctgc aaaaatgccg ggaatattgg tttccaaatg ctcgttgacc aaaacgcccc
    18001 ggtctgtttc gataccgcta tcctgaagga aggctacatt gggactgacg ccgaccgtga
    18061 ggccgacgaa accacattca atggtttcgc ctttgttggt cacaacggcc cggactttgc
    18121 cctgcgcatc ggggaggatt tctttcaatt cggtttccaa acgcaggtct atgccgtttt
    18181 cacggatgtg gcggttgatc atctgcgctt cttccaaggg cagtaccccg tcccagaagt
    18241 ttttttcccg caccaaaaac gtgaccggaa tgtggcggga gtgcagcatt tccgccattt
    18301 cgatgccaat gaggccgcca ccgacgatga ccgcccgccg aagcccggca ctgtggcgct
    18361 ccatgctttc gaggtcctgg taatgataaa gcccctgcac gccggcgagg tcctggcccg
    18421 gccagccgaa tttattggat cggctgcccg tagcaatcag tagcttgtcg aaacacagcg
    18481 tgctgccgtc ttccaagagc aggcggcgct gttccggctc tacttttttc acaaaacccc
    18541 gcagcaattc aatgcggttt ttcttccaaa accagtcctc gtaaggcttg gtatcctcga
    18601 agcggagatg ccccatataa acgtacatga gcgccgtacg ggagaagaaa tgatcggtct
    18661 cggaggacac gactgtgatg cggtgttcgc tcagcttgcg aatctggcgc gcggcggtaa
    18721 tgcccgcaat gccgtttccg atgatgacga tgtgctgctg catgtgcgtt tattttttta
    18781 caagcctgcg ttgcaaaaaa caggctcgaa gcacaatgta cagcatcgct ctggttttat
    18841 tggatgccgc cggatggatt tcatgttttt ttaatgccaa agtatttctc tgaaagccgc
    18901 acgacatttt ccaaaagaaa atcatctgat attgcacata cttttcattc atcgcaaaat
    18961 cattggctta tgaaaaagaa aactacttct caattagctc gcgtatatgt atccctaagt
    19021 ctggttttcg catctgccgg cgctcttttc tcacaacagg cactgccacc tccaccgccg
    19081 cctgactgtg gtggctttga actcgccgat ttgtggccat cgcttcgcta tggtcgaata
    19141 atcagcggta taatcacaga cctcgaaaac aaaagtttga tcggggtaaa tatttccnnn
    19201 nnnnnnngat gcatctggaa tgtacatgcc ttatggcaca gtcagtgata tggacggcgc
    19261 ttttcagttg atggtacccg ttaccgacga cgatctctac ctcacctttt cctacgtcgg
    19321 ctatgaaaaa aaatccatta aattgaagaa ggtgcatcca caacttaata tccgattgga
    19381 gtcaatagcc tatgaattgt cggaagtggt cgtaattggt tatcggacaa caatgcaaaa
    19441 aagtataaac attgattaca gttttccctc ctcgcaaaag gagcaactgt tcgttcctgc
    19501 ggctgcttgc gacctatctg tcggccaggt atatcccaat ccgttcacga ccgaattgca
    19561 tgtcaggttg gagggcaggc aggcgggcgc ggcacagctt cacctgtttg acgctgccgg
    19621 ccggtcggtg ttgtcccgaa cggagatttt gcaggaaggt atgcaaaccg tgtcattcaa
    19681 cctgaacgac taccgcttgc cgggaggagt gtattatctg cgcgtatcgg atgcagccgg
    19741 cgaaatcagt acgcacccgg tggtgagagt ggatgattga gcggtcgttt cgcgtaagcg
    19801 gagttcgagc aactcgcacc acgaggaggg aactgccaac ggcagttcga tttgaatagc
    19861 cccgcgtccg acgtaaggag gggcgcgggg tggcggtcgc gatgagtaca acccccaacg
    19921 ggggttgaat gtctgatgag cgggtgtgaa taacgacatt gaacccctgt caggggttct
    19981 tcctcccgtg atacgcatac cccgcgttca cttcgtgacg cggggctatt cactttcaac
    20041 cgccgttggc ggttgcccgc gaggcgcacg agaggcagat agtcatgaaa aatgcttcat
    20101 ttgcctcttc tttggttatt attggccctg aacttatctc tcgcttacgc gaaacgaccg
    20161 attgaaggta tagcccgacg gcaggcgcgc aagggatgga aacggcatag tcccctgaaa
    20221 gtactgttgc ctgtggggct atgagagtgg acagcccggc cccgccgcct gtattggggc
    20281 cgatgcgggg tgcgccatca tttttttgcg cttactcccg tcccagattg cgcgtgcgcc
    20341 ataccgagac gaccatgcga atggggaaca ataccccggc cacgatgctg cctaccgcga
    20401 agaggagcat ctcgccgcgc acatagccga tcatggattg cgtcatttcg taagacaaag
    20461 gccctaccaa tgtttcaggc ccgttttcgt cgaggaaggt ctggaagtgg ttcagttctt
    20521 gtattaacag gaaaaccaag ggcatacaca ggaaaagcag cacgattttg atccttcctt
    20581 tgccggccag ccaggtatgc ggcaacaaaa aaatgtagcg actgagggtg atgaacacca
    20641 cgatgaagat gagattgatg ccgaaaaaag gaaattccgg cgcccaatac cggatcggca
    20701 acagcagcgc ggcagctatc agcgccgtta aaatccacca catgatttcg aggcgcaaca
    20761 ccatgcgcat tctttccgac cccatcgtcc gttttcgttt tgatacgcgc aatacaacgc
    20821 ctgccgaaag cgggggttca atgagcgcct gccgggaggt ggttgttgat cgcctcaaaa
    20881 ccctgataaa aatcaaaaat ccggctgata attatcagtg cgccgtattt aaaaggaagg
    20941 ctacttttgg taaaaagaaa cggttcagtc cactgcaaat caatgaatac gaaccgattg
    21001 caacacaact taacaatctg aaaaacactg gcaatgaatc cggaaagacc tatccaagac
    21061 atcatgataa cggacctgat aaccgtttct cccgacgcaa gtatccgtga aatacagtac
    21121 ctttttgaag ccaatggctt ccatcacttg cccgtgatgg acaaaggaga aatgctttgc
    21181 ggcatcatca gccgggaaga ttttttcaaa ttggcctacg aactctcgct caaaaccacc
    21241 ggcaaaacct ggacggaacg cgaatacaac gccatgaagg ccaaagacat catgaccaaa
    21301 aacccgttgg tgctcgatgc agacgactcc atcgggttgg cggcagacat cttcttggcc
    21361 aatcgctttc atgccctgcc cattgtagaa gaaaaccgct tggtgggcat ggtcaccaca
    21421 cacgatctgc ttctgtacag cttcaattct tcatttattg aaaaagaaac cggagacata
    21481 ttatgatgac catagataaa accacaactg tggccgaaat catgaccgaa cgcgtcattg
    21541 ttgtgcggcc caatgagacg atggatgtcg ttcaggacat ctttcgcaaa aacaatattc
    21601 atcacgttcc ggtggtggac aaaggtatgg tggtgggtat ggtgagccat gccgactatc
    21661 tgaagctgct gcacggcttc actttgttca aaaccaaaaa aagcgatgaa tacaacgacg
    21721 ctattttgcg ctcgctcttg gttaaagagg tgatgaccaa gcaggtggct acgctggggc
    21781 cggaggattc gctggaactg gcggcgggct tctttcgcga aaacctgttt catgcgctgc
    21841 cggtagtgga tgaggagaat atgctcatcg gcattgtcac cacttatgat ttgctcaact
    21901 acgcttatgg ttgaaggaag gactgaaggg catcggctat tttgcggtca ttcgacaggc
    21961 ggggtacttt gttttgcccg ccgagtttgc ccagcgattt catatactgc cgaaaagtat
    22021 cgtgtggcaa gggtctgacg acgcaggggc gaagaatatt ccccgcaatg aggtctttgt
    22081 aataaatgtt ctgccgggtc atttcctcat ccaatgcagc ggcaa
//
LOCUS       DAORLF010000022        22023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1289_length_22023_cov_1.994446, whole genome shotgun sequence.
ACCESSION   DAORLF010000022 DAORLF010000000
VERSION     DAORLF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22023
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_22023_cov_1.994446"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PK198_03485"
     CDS             complement(<1..897)
                     /locus_tag="PK198_03485"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRF37828.1"
                     /translation="MITISKLSIFSFLYKTLLYCSLCSLAGCAVLPRGDAADAVPPVP
                     PDYSNPAYWAALPDKADPADRTPAGLSNMQASAPADVFFLHPTTYTGDRGHNQWNGPV
                     NDERLNRKTDESTILYQASLFNGVGRVFAPRYRQAHLHSYYARNAAEKAAAEKAFELA
                     WLDVKAAFEYYLAHHHQGRPIIIASHSQGTTHAKRLMREFFDGKPLQERLVAAYLVGI
                     PVEKDYLKSIPPCQTPDDTGCYCTWRSFRKGHIPKWHKDGSLIAVTNPLTWTTGEAYA
                     PADLNEGGVLYNFDKILPKLADA"
     gene            complement(1059..>2095)
                     /locus_tag="PK198_03490"
     CDS             complement(1059..>2095)
                     /locus_tag="PK198_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37829.1"
                     /translation="RHILGTQLLDSPYKLIAADVNNSKSISTLDIIQLRRLILGLTTS
                     FQGNTSWRFVDADFVFPNEANPWQTPFPELIQVGNLQGSLNGRNFIAIKVGDVNGNAI
                     LNANQVEDRSVAGTFTLYSEALDMVQNEELTVPITARNWQGIDGFQMTIEFDAEKLEF
                     LDVVYGVIGAEHVGTAMVDRGMITVSWNRQGNNVANGADPSVEGEALFWLSFKPKTGG
                     KIEQALAVNSRYARAEAYDPQGALLDVALQFERAQQKEERFELYQNQPNPFNSQTMIG
                     FRLPEDDFFTLRIHDAGGKVIKEQRGYFEAGYNQMWLHAADLPASGLVFYTLTSGTHS
                     ATRKMVIIKE"
     assembly_gap    2096..2108
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2109..>2507)
                     /locus_tag="PK198_03495"
     CDS             complement(<2109..>2507)
                     /locus_tag="PK198_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37830.1"
                     /translation="PSPNQTALVLTCDNLGTNIIEIYAWDSANNPRRVQPNGTLGGAN
                     FGFCQTFVIVQANTGVNCGGSTAMGAIAGGIFSENGNPVQSVDVNLSGYMTQSIQTEA
                     DGQYEAEDLPSGESYTVTPVCDLNAINGVST"
     assembly_gap    2508..2617
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2618..>4062)
                     /locus_tag="PK198_03500"
     CDS             complement(<2618..>4062)
                     /locus_tag="PK198_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37831.1"
                     /translation="DDVQPTAICDDNLIVAIGSNSFGRVFAQDVNEGSNDNCGIATIE
                     VRRRYDRDPETCLNVTPYYSNWGPSVDLTCCDVGRMVIIELRVTDLSGNINVCVSELT
                     VSDNTRPVCMAPPARTIACDSLPQGFNPADTLMLRNMFGAPIAVDNCIGAYSEELNPI
                     VNIADCGNGTIVRRFRARDASGNTSSNTCQQIITINLAHNYTIRFPKDAQAECGLPEV
                     DTLIVQAAGCSNMAISVTNERFTASEGACFKIFRTYRVINWCEYNGSDEPLIVNREED
                     CDNVGGDEDVWVLRRPNQTYIDRNNNHADNIPAANTRGTACGSPSNPAGYWRTVQSKG
                     YWQYTQVIKVFDTTRPTATFAAPDTACTVGTACTALVSANFTVNEGCSPNDVTVQVFA
                     DLFSDGVNDSEITGAGISGSYPSYLITGTFPIGSHRFVVNLRDGCGNTNAITIPFVVI
                     DCVGPAPVCAAGMTAVLMPLPPNTDADGDGD"
     assembly_gap    4063..4072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4073..>4725)
                     /locus_tag="PK198_03505"
     CDS             complement(<4073..>4725)
                     /locus_tag="PK198_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37832.1"
                     /translation="LKFPPLSTVIECSTNYQTTPTGNPHPSATGYPYIETAFGIYYIN
                     PVFCNLSATWQDEPRVLNDNNPNVYTFLRRWTVFNDCVPGEFTTYNQVIRVDDFSPPI
                     ISCPVGIDQNGDGTPDPPVFSTGDTECTAVVQVMPPVITDNCSNVTYMTVIISEVRVP
                     ILSPQGVILGYNTQLVPRDTIRPGQTPIVSGLPIGTHRFRYIATDARGNMSQVDCPF"
     assembly_gap    4726..4744
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4745..>5048)
                     /locus_tag="PK198_03510"
     CDS             complement(<4745..>5048)
                     /locus_tag="PK198_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37833.1"
                     /translation="ALGAYSWNIYTNFTGALNNLPITTIQDTFDLVCNDTDSLFNNPR
                     SLSITGSPIAVDNCNNVTVTFTDTRQNNGNCGPQVITRTFTATDPSGNSRTCQQTIT"
     assembly_gap    5049..5092
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5093..>5734)
                     /locus_tag="PK198_03515"
     CDS             complement(<5093..>5734)
                     /locus_tag="PK198_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37834.1"
                     /translation="TVNISNVPWPAGITFLNVPLNNQCYTVITPFMTLSDLNAYPCMD
                     DFYVVVFDVPGNPIGDTIGSEWVGFTLRAVTYSYNGLFEVESFLTFEDEIDPNITCPP
                     PATTGVITRNRQTISNTLSGADPTIALSDFSCFRGQTNPAMGNHRYDLHTFTVSAANK
                     YIFDLTPQFGRGVGVLYQGAFNPAFPCMNVIDMGRDSMLFMFMSPDSMMRLTA"
     assembly_gap    5735..5865
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5866..>6141)
                     /locus_tag="PK198_03520"
     CDS             complement(<5866..>6141)
                     /locus_tag="PK198_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37835.1"
                     /translation="PFPIGMGAQFAETAPGSGVYRLIVRHVDAVGFSLTVTNNLNESV
                     SLSAACHYPNPQIVGLNSQYCITSLPVPLQGNAGAGVAGSGTFRVNGV"
     assembly_gap    6142..6202
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6203..6463)
                     /locus_tag="PK198_03525"
     CDS             complement(<6203..6463)
                     /locus_tag="PK198_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37836.1"
                     /translation="MRHATIFTKASLNKLMLPALLASVVLLGSAGLKTNAFEAVWTFL
                     TDPPIIYRGTVSGATPPCNCLNNATNLVNGQFQDTLTIESNPG"
     gene            complement(6853..7245)
                     /locus_tag="PK198_03530"
     CDS             complement(6853..7245)
                     /locus_tag="PK198_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRF37837.1"
                     /translation="MPSIFSRIVAGEIPCHKIAETEEYLAFLDVNPRAVGHTLVIPKL
                     ETDYIFDMEDKALAGLMVFAKRVARAVEAAVPCARIGVAVIGLEVPHTHVHLVPLNRI
                     ADLSFERPPIALTQEELAATAARIRAAL"
     gene            complement(7250..7726)
                     /gene="greA"
                     /locus_tag="PK198_03535"
     CDS             complement(7250..7726)
                     /gene="greA"
                     /locus_tag="PK198_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRF37838.1"
                     /translation="MAGYNYLTKEGHDKLKAELEDLKTRGRDEAARAIAEAREKGDLS
                     ENAEYDAAKNAQGMLELRINELEKVMANARILDGSHLDESKVTVLSNVTIRNLKTMKE
                     MSYKLVSESEADLKAGRVSVSSPMGAGLLGKTVGDLAVVETPRGNMEFEIVNISID"
     assembly_gap    7936..7968
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7969..8931)
                     /locus_tag="PK198_03540"
     CDS             complement(<7969..8931)
                     /locus_tag="PK198_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRF37839.1"
                     /translation="MKKLIYTILLLPAIVGTALAGNPNRQGEAGAYELLMNPWAKSAG
                     LHTMTTANVSGVEANWINVAGMSRINKTEVAVGNADYLRGTDIRMNAFGISQRVGKKG
                     AFGLSIMALDFGDIAVTTTDQPEGTGATYSPSFFNIGFGYAHTFENKVSVGVLVRGVS
                     ETIADVGAFGFAIDAGVQYVSGDQDNFKFGISLRNIGSRMSFGGEGLSVNGPSSNGGG
                     YNLTFNQRSAGFELPSTLNIGLSYDFYLGEKNRITALGNFTANSFSEDAIGGGLEYGF
                     NNIVMLRAGYLYDFGSKDVVSAPLYAGPSAGATFELPLSKENKKT"
     gene            complement(9017..13165)
                     /locus_tag="PK198_03545"
     CDS             complement(9017..13165)
                     /locus_tag="PK198_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37840.1"
                     /translation="MLNRKLWFWNTALGLLMTLPVIANVNPNLQRSRETPAKPGNNVS
                     FRFNCDKALAQTDQAINNVRARLLTGGDVWWDAAPQNANGRYVVPKVPPGVPEVSSLF
                     AGAVWLGGLDPAGNLKVAAQTYGSSSGDKDFWPGPLDPETGGTDQTVCSKWDRFFTVK
                     GSNIELHIKNFKKAQADGVPYDPDLIPLDVKGWPGRGNPFFQEINRFALPNTRQGLAG
                     FWDEDGDMDYNPEFGDYPIIEIRGCEDSPQYPDEMTFWIYNDNGNIHSESRGDAIQME
                     VQVQAFAYATNNELNDMTFQRYKLINRAIEDIDSTFFAMWSDPDLGCYTDDYVGCDTS
                     RSLAYVYNIDAADGQTGCTCPQGVNTYCENIPILGIDYFRGPLRYIEDAQGHVIDTVE
                     IGMSSFTYFNNGGVTPTPAPGTTDPNNAQEYYNYLTGSWRDGSPFTYGGDAYQDGPPI
                     KYAFTEAPDNQTGWSMCTQALPAGDRRTIQASGPFKLRPGDINELIIGVVWVPDQRYP
                     CPSIKKLQEADDIAQDLFDACFRLPNCPDAPDVDFVELDREIIAIFTNDQNSSNNFGE
                     SYAQAGLGIPQEFADSLFVFEGYKLFQVSRPDVKLDASTLDDPSKVRLIYQVDVKNGI
                     NRIFNWRTLSDALDTPTAEEYFTPELQVDGADLGIRHTFRITEDQFAVGDRRLINHKL
                     YYFIAVAYAHNEYLPFDPVQVLGQKAPYCEGRRNIGPNGDGLPYAAVPRPITDRRLNA
                     QYGDGVVITRVDGAGTGENFLDISSETRALIEADVKADAQLGAFRGEITYTAGRGPIE
                     VKVFNPLDVKDGDFELRFVDGNMNDNVLATNARWELRRTDIPNEPLIASETTIEKLNE
                     QIIKKYGFSVTIGQTDVPGTRRDRSNGVLGYEEQYARTGANRWLTGIKDDVLPGFNLL
                     DQVVFNYVLTGTGERDEIYDPTQAFTNMGPGYFVPFYLTNWSRVGEGGPVFISPSWFE
                     SSGMNIVRTQSGLQFLNNVDVVFTSNKDLWSRCVVVETNSTFMSDAGVVSKGNRRNFD
                     VRSDSSVLKTANPATGLPVVGRLGTTDPALGMGWFPGYAIDVETGQRLNVFFGENSGY
                     RFDDQLFVTPETFNNEGPRGADMMFNPTAQMKVFPTSPEISLLQYYMGGQHFVYVTRT
                     PYDKGEFFANRFRPAPIPVLNKVAAVNQITWAGMLMTTPTAPFKSYADGLIPDDVTVK
                     LRVRAPYKVETERVINNANPRVVVPRTGTVVNNYHPAYRFSVRGKQASDLDADGVQTA
                     LDNINVVPNPYYGFSDYETSQFTNVIKVTNLPAKCVVTIYSLDGKFIRQYNRDEVGAI
                     PDGTNRGIERKQIVPDLEWDLKNTKGIPVASGVYLIHVKAPGMGERTIKWFGVNRKFD
                     PSGL"
     gene            complement(13272..>14349)
                     /locus_tag="PK198_03550"
     CDS             complement(13272..>14349)
                     /locus_tag="PK198_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37841.1"
                     /translation="RDFDPNVSFKDYEVQTNFMPRLAFSFPISEDANFFAHYDVLVQR
                     PPTNTIATALDYFYFFDRSGIRNNPNLKPERTIDYEVGFKQKLTNASALTITAYYKEM
                     RDMISSRIFFPVPIINQYETYDNLDFGTVKGFSFVFDQRRSGNFSMNANYTLQFADGT
                     GSDANSQRAISSRGVLRTLFPLNFDERHRLVLSADYRYDSGKRYTGPRLFGMDIFADA
                     GVNFQAIAVSGRPYTRKFLPTELGGEGTIGAINGARNPWNFTINLRVDKSFNIGDKMS
                     VNVYCRVSNLLDRRNIIGVYPATGSATDDGFIRSAFGQNQITSVQNSLREVDAYLASY
                     QWALLNPGNFSLPRRIFVGAIFDF"
     assembly_gap    14350..14359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14360..>16341)
                     /locus_tag="PK198_03555"
     CDS             complement(<14360..>16341)
                     /locus_tag="PK198_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37842.1"
                     /translation="QYGDVTGGIISITTKGPSQKFSGGVELETSKYFDAYANSLVGIN
                     LTGPIWKNKKTGESILGYRFSGRWTHQVDDRPPGIPVYNVTEENLKALEADPILRINN
                     SPFVAADFMKADDVDALKARPYETLRRYDVTAKIDARLSKQIDISFTGAFTNTENQFT
                     PGGWRVFNSHNNPTSFAGTRRGSVRLRHRLGGAAGGDDKKSVIQNASYSLQFGYEQEF
                     YEDYDPRHGQNHFAYGHVGNYDIQWVPTFEAMVDQTTGEVSFVHSDNRQVLRGYSNAG
                     SSNPVLSNYNNALGLDFSEGLNGQIPNVIIAGVNDGIENILRREAFIAPNGTISNQFT
                     SSWNFHTNVGTVFNRVLKSEDDVTTFNVNASFDLVPKGGAGKGRHNIQFGAWYEQRTN
                     RQHNVAPRGLWDVARQLANNHILGIPVDADGNPTSPVIGTTQVTNPVTGEEVTVDLHD
                     VQIVENAEAAFYRRLRSSLGITNLGQYVNVDGLNPSQMSLNMFSAKELNDQNILGYYG
                     YDYLGGKFDGTFEDFFTATDADGIRTFPVAPNRPIYQAFYIQDKFTFRDIIFRLGLRV
                     DRYDANTKVLKDNYSLYEIMGAGEFHDRFGGDRPGNIGDDFKVYTAGAGSDNVQAYRA
                     GDQWYLPNGTPVNNVALAIPSGVVTPKYQDARIDDN"
     assembly_gap    16342..16461
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16462..16958)
                     /locus_tag="PK198_03560"
     CDS             complement(<16462..16958)
                     /locus_tag="PK198_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF37843.1"
                     /translation="MMRNLLLISFLLLSAVAAVAQTSLGGTMTDGDSKEPVIGGTVLL
                     FKNGVQITGTTTDFDGTYSITNLDPGTYDVEASYLGYQSQRVEKVVIFAGKANKLDFV
                     MSAGVLLTTVEVKTYRVPLVEQDNTTQGQTITSEQIRNLPTRNINALAATTAGLASAD
                     EGGAIT"
     gene            17275..>17770
                     /locus_tag="PK198_03565"
     CDS             17275..>17770
                     /locus_tag="PK198_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37844.1"
                     /translation="MILLTVSEWWMAMQRPEQIFWIIAVVFTVLMLIQFILTLTGLDF
                     DGDSDAGDLSHDGGLDHDHSFSVFSVRSIIAFFAFLGWTGVLMLGAGISLWLALLLGL
                     LMGGIAMFLVAYLIFWFSRLGQEDNVTAHEAIYQAGDVYLAIPGGRSGIGRVTVKTRN
                     VLREW"
     assembly_gap    17771..17789
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17912..>18855
                     /locus_tag="PK198_03570"
     CDS             17912..>18855
                     /locus_tag="PK198_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF37845.1"
                     /translation="MGTSLIILVVGLVLLVFMTLAFVISRYRRCPSDKVLVIYGRTGS
                     GSAKCLAGGAAFVWPVIQDYEYLDLSPISIDVDLRRALSKQNIRVDVPSRFTVAISNE
                     EGVMQNAAERLLGKSMEEIRDIAKDIIFGQLRLVVATMDIEEINSDRDKFLSNVSTNV
                     GAELRKIGLALINVNITDIQDESGYIEALGKEAAAKAVNDARMSVAEKTRDGSIGEAN
                     AKKDQRIKVSEAEAFAKVGEAAANAQAVDGENQSRVTIANSQAELRIKEAEAAMQERI
                     KVSEAMSEAQVGEAAARAKAIEGENLAKITIAESEAARR"
     assembly_gap    18856..18938
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18939..>19477
                     /locus_tag="PK198_03575"
     CDS             <18939..>19477
                     /locus_tag="PK198_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HRF37846.1"
                     /translation="YLAEKKAEEARAARERATKEADIIVQAEIQRRKMEIEAEAQAEQ
                     TRRLAKGEADAIFLKQEAEARGLYEILSKQAEGFDKIVQAAGGNTRDAVLMLIADKIE
                     DLVKTQVDAIKNIKIDKVTVWDGGNHSDGEGNSTSRFVSGLYKSVPPLNDLFNMAGME
                     LPKYLGNPKAADAPASEEQ"
     assembly_gap    19478..19522
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19795..19844
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19908..>20165
                     /locus_tag="PK198_03580"
     CDS             19908..>20165
                     /locus_tag="PK198_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37847.1"
                     /translation="MEEIKARITPSALREKKSVLLELSRVNYDGEDNGLFSVVHDKMT
                     NTIRYAMAISYNDRVYYIKAFCFDQLEESFDPLIRKSFMSTY"
     assembly_gap    20166..20189
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20190..20460
                     /locus_tag="PK198_03585"
     CDS             <20190..20460
                     /locus_tag="PK198_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37848.1"
                     /translation="SMEEFRLAKFEGRNRSIQTKDGEYPTQSPDAAVIEWTTMDDLTG
                     ISETGLVENELQKLTGMERPGVSVLYLENGKYYQGTASAENIKAL"
     gene            complement(20689..20765)
                     /locus_tag="PK198_03590"
     tRNA            complement(20689..20765)
                     /locus_tag="PK198_03590"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20729..20731),aa:Ala,seq:ggc)
     gene            complement(20756..20962)
                     /locus_tag="PK198_03595"
     CDS             complement(20756..20962)
                     /locus_tag="PK198_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37849.1"
                     /translation="MAEKNIAVQASAYSGGGGSAISFVKFSKYFSSPSLDFSLFCLNF
                     ATAFEEKLKNSPAFALAMADKRGV"
     gene            21022..>21494
                     /locus_tag="PK198_03600"
     CDS             21022..>21494
                     /locus_tag="PK198_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF37850.1"
                     /translation="MSIALPKPVGVAIVDDQQLFLDGISALLNACPELEVVAAAMSGA
                     QALSLFEQRCPEIVLLDLSMPERDGMEVLNDLKAHFPEIKVIMLTVHDDLPSIQECLR
                     RGAMGYVLKINGKDELLHAILEVAGGRKYLDPKVLDTLIGHEKPPAGDGQKTLSGS"
     assembly_gap    21495..21635
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21695..>22023)
                     /locus_tag="PK198_03605"
     CDS             complement(21695..>22023)
                     /locus_tag="PK198_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF37851.1"
                     /translation="PTNLQLTPETETGLYWIAQEALNNALKHAEASQLSVQWLAHEKE
                     IVLIVEDNGKGFQSGKTAGEGIGLANVRNRVTVLGGQMNTDSQPGHGATIVVRVSNEA
                     VFRNEV"
BASE COUNT         5027 a         5417 c         5768 g         4898 t
ORIGIN      
        1 ggcatctgcc agcttgggta aaatcttgtc gaaattgtat aaaaccccgc cctcgttgag
       61 gtcggcagga gcgtacgcct cgcccgtcgt ccaagtgagc gggttggtaa cggcgatgag
      121 gctgccgtct ttgtgccatt tgggaatatg ccctttgcga aagctgcgcc aggtgcaata
      181 gcagccggta tcgtcgggcg tctggcaggg cggaatggac ttcaaataat ctttttctac
      241 gggaatgccc acgaggtagg ccgccacgag ccgttcttgc aagggcttgc cgtcgaagaa
      301 ctcgcgcatc agacgtttgg catgcgtagt accctgactg tgcgaggcga tgatgatagg
      361 ccgcccctga tgatggtggg ccagatagta ttcaaatgca gctttcacgt cgagccaggc
      421 caattcaaat gccttttcgg cggctgcttt ttcggcggca ttgcgcgcat aatatgagtg
      481 caaatgcgcc tgcctgtagc gcggagcgaa gacccgtccc acgccgttga acaggctggc
      541 ctgatacaaa atagtacttt cgtcggtttt gcggttcagg cgctcgtcgt tcaccgggcc
      601 attccattgg ttgtggccgc ggtcgccggt gtaggtggta ggatgcagaa aaaacacatc
      661 ggcgggggcg cttgcctgca tgttggagag gccggcgggc gttcggtcgg cggggtcggc
      721 tttatcgggc agggcagccc agtaagccgg gttggaatag tcgggcggaa cgggcggcac
      781 agcatcggcg gcatcgcccc tcggtagcac ggcgcagccg gcgaggctac acaatgagca
      841 gtaaagcagt gttttgtata aaaagctgaa tatagacagt ttggagatgg tgatcattgt
      901 tggatggtct ttacagtgga taagaatcat tggtaaagat agtttggaaa ttgatttgaa
      961 ggtacaaata tcgtgaaaaa ccaaaagggc gaccctccca tcggagagcc gcccgctttt
     1021 gaagttggta tcattattcc aacaaatccc tcatcatgtt attcctttat gatcaccatt
     1081 ttgcgcgtgg cagaatgggt gcctgaagtc agggtgtaaa agaccagacc ggaagccggg
     1141 aggtcggcgg catgtagcca catttggttg tagccagcct cgaaataacc gcgttgttcc
     1201 ttgatgactt taccgcctgc atcgtgtatg cgcagggtga agaagtcgtc ttcgggcagc
     1261 ctgaaaccga tcatggtttg gctattgaac gggttgggct gattttggta caactcgaag
     1321 cgctcttctt tttgctgtgc gcgttcaaat tgcagcgcca catccaacag cgcgccttgc
     1381 ggatcataag cctcggcacg ggcgtagcgg gagttgacag ccaatgcctg ctctatctta
     1441 ccgcctgtct tgggtttgaa cgacagccag aacaatgcct cgccttccac tgaggggtcg
     1501 gcgccattag ccacgttgtt gccctgacgg ttccagctca cggtaatcat gccgcggtcc
     1561 accatagcag tgcctacatg ctctgcaccg atgacgccat atactacatc gaggaactcc
     1621 agcttttctg catcaaactc aatcgtcatt tggaaaccgt ctatgccttg ccagttgcgg
     1681 gccgtgatgg gcacggtcag ttcttcattt tgtaccatgt caagtgcttc tgagtacagc
     1741 gtgaaagtac ctgccacgct gcggtcttcc acctgattgg cattgaggat ggcgttgccg
     1801 ttcacgtcgc ccactttgat ggcgatgaag ttgcgcccat tgaggctgcc ttgcaggttg
     1861 cccacctgaa tgagttcagg gaagggcgtt tgccaggggt tggcctcatt ggggaacaca
     1921 aagtcggcgt ccacaaatcg ccagctcgta ttgccctgga agctggtggt gaggcccaga
     1981 atcaggcggc gcaactgtat gatatctaat gttgaaatag acttggaatt gttcacatcg
     2041 gcagcgatga gcttgtacgg cgagtcgagc aattgcgtac ccaaaatatg gcgggnnnnn
     2101 nnnnnnnngt agaaacccca ttgatggcat tgagatcaca caccggcgtc acagtgtatg
     2161 attcaccgct gggcaggtct tctgcttcat attggccatc ggcctctgtc tgaatacttt
     2221 gcgtcatgta cccgctcagg ttcacgtcca ccgactgaac cgggttgccg ttttcactaa
     2281 aaataccgcc tgcaatagcg cccatggccg tagagccgcc acagttgaca ccggtattgg
     2341 cctgcacgat gacgaaagtc tggcagaatc caaagtttgc tccgcccagc gtgccgttgg
     2401 gctgcacccg gcgcgggttg ttggcgctgt cccaggcata aatttcgatg atattggtac
     2461 ccagattgtc gcaggtcaac accaaagcag tttggttggg cgatggannn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag tcgccgtcgc cgtcggcatc
     2641 ggtgttcggc ggcaagggca tgagcacagc cgtcatgccg gcagcgcaaa cgggcgcagg
     2701 gccaacgcag tcaatcacga caaaaggaat ggtaatggcg ttggtattgc cgcacccgtc
     2761 gcggaggttg accacaaaac ggtggctgcc gatggggaag gtgcccgtaa ttaagtagct
     2821 cggatagctg cccgaaatac ccgctcctgt gatctcgctg tcgtttacgc cgtctgagaa
     2881 caaatctgcg aatacttgca cggtaacgtc attgggcgag cagccttcat tgaccgtaaa
     2941 attggccgag accaaagcgg tgcaggcagt gcccactgtg caggccgtat caggcgctgc
     3001 gaaagtagct gtcggacgag tcgtatcaaa cactttgatg acttgcgtgt attgccagta
     3061 ccctttagat tgtacggttc gccagtaccc tgccggatta cttggactac cgcaggccgt
     3121 gccgcgtgta ttggccgccg gtatgttgtc ggcgtggttg ttgttgcgat ctatataggt
     3181 ctgatttggg cgacgcaata cccacacatc ttcatctcct cctacgttat cgcaatcctc
     3241 ttctctgttg actatcaacg gttcatcgct tccgttgtac tcgcaccagt tgatgacccg
     3301 gtacgttctg aatattttga aacaagcgcc ctccgaggct gtgaaccgct cattggtaac
     3361 gctgatggcc atattggaac agcccgcagc ttgaacaata agcgtatcca cttccggcaa
     3421 gccgcactct gcctgagcat ctttggggaa gcgaatcgtg tagttgtgtg ccaaattaat
     3481 ggtaatgatc tgctggcagg tgttggaaga ggtattgccc gatgcatctc tggcccggaa
     3541 acggcgcaca atggtaccgt tgccgcaatc ggcgatgttc acaattgggt tcaactcttc
     3601 cgaataggcg ccgatgcagt tgtccaccgc aatgggtgcg ccgaacatat tgcgcagcat
     3661 cagcgtatcc gccgggttga aaccttgtgg cagcgagtcg caagcgatgg tacgtgccgg
     3721 cggcgccata cacaccgggc gggtattatc gctcacggtc aattcgctga cgcatacgtt
     3781 gatattgccc gacaagtcgg ttacgcgcag ttcaatgatg accattctgc ctacgtcaca
     3841 gcaagtaagg tccaccgagg gtccccagtt ggagtagtaa ggcgtcacat tgagacaggt
     3901 ttccgggtcg cggtcgtatc tgcgccgtac ttctatggtg gcaataccgc agttgtcgtt
     3961 gctgccttcg tttacatcct gagcgaatac gcgcccgaag ctgttgctgc cgatggctac
     4021 aatcagattg tcgtcgcata tagcggtagg ctgtacatca tcnnnnnnnn nngaacgggc
     4081 agtccacctg gctcatattc cctctggcat cggtggcaat gtaccggaag cggtgcgtac
     4141 cgatggggag gccgctcacg atgggggttt gccccggccg gatggtatcg cgtggaacga
     4201 gctgcgtgtt gtaacccaat atcacaccct gcggactgag aatgggcacc cttacctcag
     4261 agatgatgac ggtcatgtag gtcacgttgc tgcaattatc ggtgatgacc ggcggcatta
     4321 cctgcactac ggcggtacat tctgtgtcgc ccgtggagaa taccggcgga tcgggggtgc
     4381 cgtcgccatt ctgatcaata cccaccgggc aggaaatgat cgggggcgag aagtcgtcca
     4441 cgcgaatcac ctgattgtag gtagtaaatt cgccgggcac gcagtcgttg aacaccgtcc
     4501 agcggcgcaa gaaagtataa acgttcgggt tgttatcgtt cagcaccctc ggttcgtctt
     4561 gccaggtagc cgacaggttg caaaataccg gattgatgta gtaaatgcca aaagccgttt
     4621 caatgtaagg atacccggta gcagaggggt gcggattgcc ggtaggcgtt gtctggtagt
     4681 tggtgctgca ctcaatgacc gtgctgagcg gcggaaattt gaggtnnnnn nnnnnnnnnn
     4741 nnnngtgatg gtttgctggc aggtgcggct gttgcccgat gggtcggtag ctgtaaatgt
     4801 gcgcgtaatg acttgcgggc cgcagttgcc gttgttctgg cgggtatctg taaacgttac
     4861 cgtcacgttg ttgcagttat caacagcaat gggcgacccg gtgatggaca agctgcgcgg
     4921 gttgttgaaa agtgagtcgg tatcgttgca aaccaaatca aaagtgtctt gaatggtggt
     4981 aatcggcagg ttgttgagcg cgcccgtgaa atttgtatag atattccagg agtatgctcc
     5041 caaagcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggccgtc
     5101 aggcgcatca tcgaatcggg ggacataaac ataaagagca tgctgtcgcg gcccatgtct
     5161 atcacgttca tacacggaaa cgccggattg aacgcgccct ggtacaacac gcccacccct
     5221 ctgccgaact gcggcgtcag gtcgaagata tacttgttcg cagcacttac cgtgaaggtg
     5281 tggaggtcgt agcggtggtt gcccatagcg gggttcgtct ggccccggaa acaactgaaa
     5341 tccgaaagcg caatcgtcgg gtctgcacca gacaaggtgt tggaaatcgt ttgccggttt
     5401 ctcgtgatga cacccgtagt tgccggcggc gggcaggtga tattggggtc tatttcatcc
     5461 tcaaaagtga ggaagctttc cacctcaaaa agaccattat atgaatacgt tacagcgcgc
     5521 aacgtaaatc ctacccattc tgaaccaata gtgtcgccta tggggttgcc cggcacatca
     5581 aataccacca catagaagtc gtccatgcag gggtaggcat tgaggtcgga caaagtcata
     5641 aacggcgtta tcaccgtgta gcactggttg ttgagcggca cgttgaggaa ggtaatgccc
     5701 gccggccagg gtacattgct gatgtttacg gtttnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacgc cgttaacccg
     5881 gaacgtaccg ctacctgcta cccccgcgcc ggcgttgcct tgcagcggca ccggtaaact
     5941 ggtgatgcag tattggctgt tcagacccac tatctgcgga ttggggtagt ggcaggcggc
     6001 actcaggctc acgctttcgt tgaggttgtt ggtcaccgtc agcgaaaaac caactgcatc
     6061 cacatgcctc acaatcaggc gataaacacc cgatccgggg gccgtttcag caaactgcgc
     6121 tcccatcccg ataggaaaag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nntcccggat tggactcgat ggtgagtgtg tcctgaaact
     6241 gcccgtttac caagttcgtg gcgttgttga ggcagttgca gggcggcgtt gcaccggaga
     6301 cggtgccccg gtaaatgatc ggcgggtccg tcaaaaaagt ccataccgct tcaaaagcgt
     6361 tggtcttcag gcccgcactc cccaataaca cgaccgaagc gagcagcgcc ggcagcatca
     6421 gtttgttcag ggaggctttt gtgaaaattg ttgcgtgtct catagcacaa cattgttttg
     6481 gatgatgaag gaataaaccc cggttcattt cagacataag gatgactggc cgccgtacat
     6541 acgcggtact tcagccgcac atccttttgt cggtacgttt cccggaaaat tttgggatga
     6601 actaactacc cttcatctaa accaaaaatg ataccaattt taaaaaagac aggatacagc
     6661 gccataaaaa acacacaaaa cgctgtaaat caatgtataa aaaaatatat aaattttcat
     6721 aactactatt cagcaccttg cccaaagcca taccaacggc agcgccaccc tccatagcgc
     6781 cctgaaatac aggcacaaaa aaacgggtac ccacctaagc agatacccgt ttcccggaaa
     6841 ctcaagttgc cgttacagcg cggcccggat tcgagcggcg gtcgcagcca gttcctcttg
     6901 tgtgagggca atcggcggac gctcgaaact cagatcagcg atgcgattga gcggcaccaa
     6961 atgtacatgc gtatgcggca cttccaaacc aatgaccgcc accccgatgc gggcgcaggg
     7021 tacagccgcc tcaacagcgc gggctacgcg ctttgcaaac accatcaagc ccgccagcgc
     7081 cttgtcttcc atgtcgaaga tgtaatcggt ctccaacttg ggaatgacca acgtgtgccc
     7141 cacagcgcgc ggattgacat ccaaaaaggc cagatattct tctgtttcgg caattttgtg
     7201 gcaaggaatc tcgccggcta caatacggga gaaaatgctc ggcatgagat caatcaattg
     7261 agatattgac aatttcaaat tccatattgc cacgcggcgt ttctaccaca gccaaatcgc
     7321 ctaccgtctt gcccaacaac ccggcgccca taggcgagct gaccgacaca cggccagcct
     7381 tcaggtcggc ctccgactcg gaaaccaatt tataagacat ttctttcatg gtcttcaagt
     7441 tgcggatggt cacattggac agtaccgtca cttttgactc atccagatgc gaaccatcta
     7501 aaattctggc attggccatc actttttcca actcattgat gcgcaactcc agcatgccct
     7561 gtgcattttt ggcggcatca tattctgcat tttccgaaag gtcgcctttt tcgcgagcct
     7621 cggcaatggc gcgggcggct tcatcacggc ctctggtttt gaggtcttcc aattccgctt
     7681 tcagtttgtc atgtccttcc ttggtcaaat aattgtaccc tgccatagga aattcgtttt
     7741 attcggttga taaaaattaa aaaagacaaa aacgctccca cagcattgcg gaagcgtttt
     7801 tgcctttgat tgttatttag aagtgcaaag atacgcacac tttcagcaaa acacaagctg
     7861 tgagcgtaat ttttgcaacg caggcattac atattcagac gaacgccgat gttatgcgta
     7921 ccgttgaaaa tgttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ttttcttgtt
     7981 ttccttgctc aggggcaact caaaagtagc gcccgcgctg gggcctgcat acaaaggagc
     8041 agacactacg tctttagagc caaaatcgta tagataacct gcgcgcaaca taacgatgtt
     8101 gttgaaccca tactccaaac caccgccaat ggcgtcttcc gaaaaggagt tggctgtaaa
     8161 attgcctaat gctgtaatcc ggtttttctc gcccagataa aaatcataag acaacccgat
     8221 gttcaaggta gaaggaagtt caaaaccggc agaacgctga ttgaaggtaa gattgtaacc
     8281 tccgccattg gaactggggc catttaccga caagccctcg cctccaaaac tcatgcggga
     8341 accgatattg cgaagcgaaa tgccgaactt gaaattatcc tgatctcctg acacatactg
     8401 cacccccgca tcaatggcaa atccgaaagc gcctacgtcg gcgatggttt cagacacgcc
     8461 gcgcaccaac acgcctaccg acactttgtt ttcaaaagta tgggcatatc caaagccgat
     8521 gttgaaaaat gaaggagagt atgtagcgcc ggtaccttca ggctgatcgg tagtcgttac
     8581 cgcaatatcg ccgaaatcca gggccatgat gctgaggccg aaggctccct ttttgcccac
     8641 gcgctgcgaa ataccgaagg cattcatacg aatatcggtg ccgcgcaggt agtcggcatt
     8701 gcctactgct acttctgttt tgttgatgcg cgacatgccc gccacgttga tccagttggc
     8761 ctccacaccc gacacatttg cggtggtcat ggtatgcaaa ccggcactct tggcccaggg
     8821 attcatcagc aactcatacg cgccggcttc cccctgacgg ttggggttgc ccgctaaggc
     8881 ggttcccaca atggccggca acaagagtat ggtgtagatt aattttttca tgctcgatgt
     8941 ttgaagtaaa ataaaaagaa aaagaagaga gccggtcttc cttgcggtat caaaccggct
     9001 ctccaatgat tgttgattac aaaccggagg ggtcaaactt gcggttcacc ccgaaccatt
     9061 tgatcgtgcg ttcgcccata cccggcgctt taacgtggat gagatatacg ccgctggcca
     9121 ccggaatgcc cttggtgttt ttcaaatccc attccaaatc gggtacaatt tgcttgcgct
     9181 caatgccgcg attcgtgccg tccggaattg cccccacttc gtcgcggttg tattggcgaa
     9241 tgaacttgcc gtcaagcgag taaatcgtca ccacgcattt ggccggcaaa ttggtcacct
     9301 tgatgacatt cgtaaactgc gacgtttcgt agtcggagaa accataatac gggttgggta
     9361 ctacattgat gttgtccaaa gcggtttgta cgccgtcggc atccagatcg gaagcctgct
     9421 tgcccctgac gctgaagcgg taggccgggt gataattgtt caccaccgtg ccggtgcgcg
     9481 gcaccactac gcgcgggttg gcattattga tcacgcgctc ggtttccacc ttgtaggggg
     9541 cgcgaacacg gagcttcacc gtcacatcat cggggatcaa accatcagcg taggacttga
     9601 acggcgccgt cggcgtggtc atcagcatac ctgcccaggt aatctggttc acggcagcta
     9661 ccttgttcag cacaggtatg ggcgccgggc ggaaacgatt tgcaaagaat tcgcccttgt
     9721 cataaggtgt gcgagtgacg taaacaaagt gctgaccgcc catatagtat tgaaggagac
     9781 tgatctccgg actggtaggg aacaccttca tttgtgccgt cggattgaac atcatgtcgg
     9841 cgccgcgagg gccttcattg ttgaaggtct ccggtgtaac aaacaactga tcgtcaaaac
     9901 gataccccga gttttcacca aagaaaacat tgagtcgctg tcccgtctcc acatctatgg
     9961 cgtaaccggg gaaccagccc atgccgagag cagggtcggt agttccgagc cttcccacta
    10021 ccggtaagcc ggtcgcgggg ttggccgttt taagcacaga ggaatcagaa cgcacgtcga
    10081 agtttctgcg gttgcctttg gaaactacgc ccgcatccga cataaaagtg ctgttcgtct
    10141 ctaccaccac gcaacggctc cagaggtctt tattggaggt aaacacgacg tccacattgt
    10201 tcaggaactg aagcccggac tgcgtgcgca cgatgttcat accggaagac tcaaaccagc
    10261 tcggggagat gaataccggc cctccttctc caacacgcga ccagttggtc aaatagaacg
    10321 gtacaaagta acccggcccc atgttggtga atgcctgtgt gggatcgtaa atctcatccc
    10381 gctcgccggt accagtcagc acatagttga atacgacttg gtccaagagg ttaaagcccg
    10441 gcaaaacgtc gtcttttatg cctgtgagcc aacggttggc gcctgtacga gcatattgct
    10501 cttcataacc caacacgccg ttggagcggt cgcgtcgggt gccgggcaca tctgtctgac
    10561 caatggtgac actgaatcca tatttcttga tgatctgctc attgagtttc tcgatggtcg
    10621 tttccgatgc aatgagcggt tcgttgggaa tatccgtacg acgcagttcc caacgcgcgt
    10681 ttgttgccaa tacgttgtca ttcatattgc cgtccacaaa acgcagttca aagtcgccgt
    10741 cttttacatc caaggggttg aataccttca cctcgatagg accgcgacca gccgtataag
    10801 tgatctcgcc gcggaaagcg ccgagttgag catccgcctt gacatccgcc tcaatgagtg
    10861 cgcgggtctc gctggaaata tctaagaagt tttcgccggt accagctcca tccacacggg
    10921 taatgaccac gccgtcgccg tattgggcat tgaggcggcg gtcggtgatg gggcgcggca
    10981 cagcagcgta gggcaagccg tcaccgttgg ggccgatgtt gcgacggcct tcacagtagg
    11041 gtgctttttg gcccaatacc tgtaccggat cgaaaggcaa gtattcattg tgagcatacg
    11101 ctaccgcaat gaaatagtac agtttgtgat tgatcaaacg gcgatcaccc acggcaaatt
    11161 ggtcttccgt aatgcggaaa gtatggcgta tgccgaggtc ggcgccgtcc acctgcaact
    11221 ccggcgtgaa gtattcttca gccgtcggcg tatccaatgc atcgctcaaa gttctccagt
    11281 tgaagatgcg attgatgccg tttttcacgt ccacctgata gatgagacgc actttagatg
    11341 ggtcatccag agtagaagca tccaacttga catccgggcg ggaaacctga aacagcttgt
    11401 agccctcgaa tacaaacagc gagtctgcaa attcttgcgg aatccccaaa cctgcctgtg
    11461 catagctttc accgaagttg ttggaggaat tctggtcatt ggtaaaaatg gctatgatct
    11521 cgcgatccaa ttccacgaag tccacatcgg gtgcatccgg gcagttgggc aatctgaaac
    11581 aagcgtcgaa caagtcttgt gcgatgtcgt cggcttcctg caacttcttg atgctgggac
    11641 aagggtaacg ctgatcgggc acccatacca cgccaatgat gagttcgttg atgtcgccgg
    11701 gacgcaattt gaacggcccc gaagcctgaa tggtacggcg gtcgccggcg ggcagggcct
    11761 gcgtacacat gctccagcct gtttgattgt cgggcgcttc cgtgaacgcg tacttgatcg
    11821 gcgggccatc ctgataagca tcgccgccat aggtaaacgg cgagccgtcg cgccaggaac
    11881 ctgtcagata gttgtagtat tcctgcgcgt tgttcgggtc ggttgtgccc ggcgcgggcg
    11941 tgggcgtcac accgccgttg ttgaagtatg tgaacgaaga catgcctatt tctactgtgt
    12001 caatgacatg tccttgggca tcctcaatgt agcgcaaggg gccccggaaa tagtcaatgc
    12061 ccagaatagg aatgttttca caataagtat tcaccccttg cgggcaggta catccggttt
    12121 gtccatcggc ggcgtcaatg ttatatacat aagccaaact ccgggaagta tcgcagccca
    12181 catagtcgtc ggtgtagcaa cccaagtcgg ggtcggacca catggcaaag aaagtactgt
    12241 caatatcctc aatagcgcgg ttgatgagct tgtagcgctg gaaggtcatg tcgttgagtt
    12301 cgttgttggt agcataagca aaagcctgca cctgcacttc catctggatg gcgtcgccgc
    12361 gagattccga gtggatgttg ccgttgtcgt tgtaaatcca gaaggtcatc tcatccggat
    12421 attgcgggct gtcttcgcag ccccggattt cgatgatggg atagtcgcca aattccgggt
    12481 tgtaatccat atcgccgtct tcatcccaga agccggccaa accctggcgc gtattgggca
    12541 atgcaaaacg gttgatttcc tgaaagaacg ggttgccgcg gccgggccag cccttcacat
    12601 ccaaaggaat gagatcggga tcgtagggga cgccgtctgc ctgcgctttt ttgaaatttt
    12661 tgatgtgcaa ctcaatgtta ctgcctttca cagtgaaaaa gcggtcccac ttggagcata
    12721 cagtttgatc ggtgccgccg gtttcggggt ctaatgggcc aggccagaag tctttgtctc
    12781 cggaagaaga accatacgtc tgcgcggcta ctttcaggtt gccggcaggg tccaaaccgc
    12841 cgagccatac agcgccggca aacaaagaag aaacttccgg cacgccgggt ggcactttgg
    12901 gcaccacata ccggccgttg gcgttttgcg gagctgcatc ccaccataca tcaccgccgg
    12961 tgagcaaacg cgcacgtacg ttgttgatgg cctgatcggt ttgcgcaagg gctttgtcgc
    13021 agttgaatcg gaaagaaacg ttgttgcccg gtttagccgg tgtttctctg ctccgctgta
    13081 agtttggatt cacattggct atgaccggca aggtcatcaa caggcccaga gccgtgttcc
    13141 aaaaccaaag ttttctgttg agcatattag aaaaagttta aaagtcaaca attttgaaag
    13201 tcaattcaaa aaaaagtcag agtgagtgaa gtcaggcggt aacttccggt tacctcctga
    13261 cttcaatacg attagaaatc aaaaatagcg cctacaaaaa tacggcgtgg caggctgaaa
    13321 ttgcccggat tgagcaaggc ccactggtaa gatgccagat aagcatccac ttcgcgcaat
    13381 gaattttgta cgctcgtaat ttggttttgc ccgaaagccg aacgaatgaa gccgtcatcg
    13441 gtagccgagc cggtagcagg atatacgcca atgatgttgc ggcgatccaa gaggttggaa
    13501 acgcggcaat aaacattcac cgacatttta tccccaatat tgaagctctt gtccacgcgc
    13561 aagtttatcg taaaattcca agggttgcgc gcgccgttga tggcgccgat ggtaccctcg
    13621 ccgcctaact ccgtaggcag aaacttacgg gtataaggac ggccggaaac agcaatggcc
    13681 tggaagttga cgcctgcatc ggcgaagatg tccataccga acaaacgcgg accggtgtag
    13741 cgtttgccgc tgtcgtagcg atagtcggca ctgagtacca aacggtgacg ttcgtcaaaa
    13801 ttgagcggga acagggtgcg caacacaccg cggctggaaa tggcgcgctg agagttggcg
    13861 tcggagccgg taccgtcggc aaattgcagc gtatagttgg cattcatgga gaaattgccc
    13921 gaacggcgct gatcgaacac aaaagagaaa cctttcaccg taccgaagtc gaggttgtcg
    13981 taggtttcgt attggttgat gatgggcacg gggaagaaaa tccgcgaaga aatcatgtcg
    14041 cgcatttctt tgtaatatgc cgtaatggtg agcgccgaag cattggtcag cttttgtttg
    14101 aaacccacct catagtcaat ggtacgctcg ggcttgaggt tggggttgtt gcggatgccg
    14161 ctgcggtcga agaagtagaa atagtcaagc gctgtggcga tggtgttggt cggcgggcgc
    14221 tgcaccaaca cgtcgtagtg ggcgaagaag ttcgcatctt cggaaatcgg gaacgagaaa
    14281 gcgagacgcg gcatgaagtt ggtttgcact tcgtagtcct tgaagctcac gttcggatcg
    14341 aaatcgcggn nnnnnnnnna tgttgtcgtc aatgcgggca tcctgatatt tgggcgttac
    14401 cacgccggaa ggaatggcca gggccacgtt gttcacgggc gtgccgttgg gcaggtacca
    14461 ttggtcaccg gcgcgatagg cttgcacatt gtcgctgccg gcgccggcgg tatatacttt
    14521 gaagtcgtcg ccgatattgc cgggacgatc accaccaaaa cgatcatgaa actcacctgc
    14581 gcccatgatt tcgtacagcg aataattgtc tttgagcacc ttggtattgg cgtcgtagcg
    14641 gtcaacgcgc aaaccgaggc ggaagatgat gtcgcggaaa gtgaacttgt cctgaatgta
    14701 gaatgcctgg taaatcgggc ggttgggcgc taccgggaaa gtgcggatac catcagcatc
    14761 ggtagctgtg aagaaatctt caaaggtgcc gtcaaatttt ccgccgaggt agtcataacc
    14821 atagtagccc aagatgttct ggtcattcaa ttctttggca gagaacatat tgaggctcat
    14881 ctggctcgga ttgaggccgt ccacatttac atattgcccc aggttggtga tacccaaaga
    14941 actgcgcagg cggcggtaaa atgcggcttc ggcgttttcc acgatttgta catcgtgaag
    15001 gtcaacggtc acttcttcac ccgtcaccgg gttggtcact tgcgtggtgc cgatcacagg
    15061 agaagtcggg ttgccgtctg cgtccaccgg aataccgaga atatggttgt tggccaattg
    15121 gcgggccaca tcccacaaac cgcggggcgc tacgttgtgc tggcggttgg tgcgctgctc
    15181 ataccaggcg ccgaactgga tgttgtggcg gcctttacct gcgccacctt tcggaacgag
    15241 gtcgaacgaa gcattgacgt tgaaggtcgt tacatcatct tccgacttga gtacgcgatt
    15301 gaataccgtg cctacattgg tgtggaagtt ccaggaagaa gtgaactggt tggaaatggt
    15361 gccgttgggc gcaatgaagg cttcccggcg gagaatgttc tcaatgccgt cgtttacacc
    15421 ggcaatgatg acattgggaa tctgcccgtt caggccctcc gaaaagtcaa ggcccaatgc
    15481 gttgttgtaa ttggacaata ccggattgga agacccggca ttggaatagc cgcgcagcac
    15541 ctgacggtta tccgagtgga caaacgaaac ttctccagtt gtctgatcca ccattgcttc
    15601 aaacgtgggc acccattgta tgtcgtaatt gcctacatgg ccataagcaa aatggttttg
    15661 gccgtggcgc ggatcgtagt cttcgtaaaa ctcttgctcg tagccgaatt gcaaagaata
    15721 cgaagcattc tgaataaccg attttttatc gtcgccaccc gcagcgccgc ccaaacggtg
    15781 gcgcaggcga acggagccgc gacgcgtgcc ggcgaaagac gtagggttgt tgtgactatt
    15841 gaacacgcgc cagccaccgg gcgtaaactg gttttccgtg ttggtaaatg cccccgtaaa
    15901 cgaaatgtca atctgcttgc tgaggcgtgc gtcaattttg gcggtcacgt cgtaacggcg
    15961 cagcgtttca taggggcgcg ctttgagcgc atccacatca tcggctttca taaagtcggc
    16021 ggccacaaag gggctgttat tgatgcggag gatagggtcg gcttcgagtg ccttgagatt
    16081 ctcctccgtt acattgtaca cggggattcc aggcggacgg tcatccacct gatgcgtcca
    16141 gcggccggag aagcgatagc cgagaatgga ctccccggtc tttttgtttt tccagatcgg
    16201 accggtgagg ttgataccca ccaagctgtt ggcataagcg tcgaagtatt tggaagtttc
    16261 cagttccaca ccgcccgaaa acttctgtga agggcctttg gtggtaatgg aaatgatacc
    16321 gcccgtcacg tcgccatact gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn ngtgatggcc ccaccttcat cggcggaagc cagacccgcc
    16501 gtggtggctg ccagcgcgtt gatgttgcgc gtgggcaggt tgcggatttg ctcgctggtg
    16561 atggtttggc cctgcgtggt attgtcctgc tccaccaaag gcacccggta ggttttgacc
    16621 tcaacggtgg tgaggagtac tccggcagac atgacaaagt ccaatttgtt ggccttgccg
    16681 gcaaaaatga ccaccttttc aacccgctgg ctctggtagc ccagatagct ggcctctaca
    16741 tcgtaggtgc ccggatcaag gttggtaatg ctgtaagtgc cgtcgaaatc ggtagtagta
    16801 cctgtaattt gtaccccgtt tttgaacagg agtaccgtac cgccgatgac cggctctttg
    16861 gagtcaccgt ccgtcatggt tccacccagc gacgtttgcg ctacagcggc cacagccgac
    16921 aacagtaaaa agctgattaa gagtaagttt cgcatcatgc atcatttatt taaatctact
    16981 ctggcaatag gtttaccaat caagggcaca atgttaagca aatgtgtggg aacagccaaa
    17041 attttatggc ttcgtatgtc cgacagggga caaaaaatga gcgtattgct tttattttcg
    17101 agccggggac aatgaaaaac aaaagcccgc tttgtaccgc cgcatgatga atttcccaaa
    17161 acagatttac aatttcgccg ataaaatacc ccctttcatc gaatcaatac acgagacagc
    17221 aagaccattt tacattttgc gggccataat acaaaggctt ttcaaccctc aaagatgata
    17281 ctactgacgg tgagcgaatg gtggatggcc atgcaaaggc cggaacagat tttctggatc
    17341 attgccgtgg tctttacggt gctgatgctc atacagttta ttctgacact cacggggttg
    17401 gattttgacg gagactccga cgccggagac ctgagccatg acggcggatt agaccacgac
    17461 cactcttttt cggttttttc ggtgcgcagt atcatcgcgt tttttgcttt cctcggctgg
    17521 acgggcgtac tcatgctcgg cgcgggtatc agcctgtggc tggcgctgtt gctgggcctg
    17581 ctcatgggcg gcatcgccat gtttctggtg gcttacctca ttttctggtt ttcacggctg
    17641 ggccaggaag acaacgtgac ggcgcatgaa gccatctatc aagccggcga tgtatatctc
    17701 gccatacccg gcgggcgcag cggtataggc cgcgtcaccg taaaaacccg caacgtactc
    17761 cgcgaatggg nnnnnnnnnn nnnnnnnnng taacggacgg cccctacatc cccaccggca
    17821 cgaaaattct cgtgaccgac gtgctgcccg acaatatttt gttggtgacc cggcaagact
    17881 gaattacatc atcataaaac gcttcaatcc tatgggaact tctctgatta tcctcgtcgt
    17941 aggactggtt ttactggttt ttatgactct cgcttttgtt atttcccgct atcggcgatg
    18001 cccttccgac aaagtgctcg tcatttacgg gcgcacgggt tccggctcgg ccaaatgtct
    18061 ggcgggcgga gcggcattcg tatggccggt cattcaggac tacgaatact tagacctctc
    18121 ccctatttcc atagatgtgg acctgcggcg cgcactgagt aagcaaaaca tccgcgtgga
    18181 cgtgccctcg cgctttactg tggccatttc caacgaagaa ggcgtgatgc aaaacgccgc
    18241 cgaacgcctg ctgggcaagt ctatggaaga aatccgcgac attgccaaag acatcatttt
    18301 tggtcagctc cgcctcgtgg tagccaccat ggatattgag gaaatcaact ccgaccgcga
    18361 caaattcctc tccaatgtct ctaccaacgt gggcgccgaa ctcagaaaaa tcggcctcgc
    18421 gctcatcaac gtgaacatca ccgacattca ggatgaatcg ggctacatag aagcattggg
    18481 caaagaggct gccgcaaaag ctgtgaacga cgcccgcatg agcgtagcag aaaaaacccg
    18541 cgacggctcc atcggtgaag ccaacgccaa aaaagaccag cgcatcaagg tatcggaagc
    18601 agaagccttt gccaaagtgg gcgaggcggc tgccaatgcc caggccgtgg atggtgaaaa
    18661 ccaaagccgc gtgaccatcg ccaattcgca ggccgaactg cgcatcaaag aggcagaggc
    18721 cgccatgcag gagcgcatca aggtatcgga ggcaatgtct gaagcgcagg tgggcgaggc
    18781 ggcggcccgc gccaaggcca tcgagggtga aaacttagcc aaaatcacca ttgcagaatc
    18841 ggaagcggcc cgccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tatctggcag agaaaaaggc
    18961 cgaagaggct cgcgccgccc gcgaacgcgc cactaaagaa gcagacatca tcgtgcaggc
    19021 cgaaatacag cgccgcaaaa tggaaatcga agccgaagcg caggccgaac aaacccgccg
    19081 cttggccaag ggcgaggccg atgccatctt cctcaagcag gaagccgagg cccgcggttt
    19141 gtatgagata ctcagcaagc aggccgaggg ttttgacaaa atcgtgcagg cggccggcgg
    19201 caatacccgc gacgccgtgc tgatgctgat tgccgacaag atcgaagact tagtgaaaac
    19261 gcaggtggat gctatcaaga acatcaagat agacaaagtg accgtgtggg acggtggcaa
    19321 ccactccgac ggcgagggca attctacctc ccgctttgta tcggggctgt ataaatcggt
    19381 gccgccgctc aacgacctgt tcaacatggc gggcatggaa ctgcccaaat acctcggcaa
    19441 ccccaaagct gccgatgcgc ctgcaagcga agagcaannn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnctgttacc tcctgacttc gcccgcagca aatgggcccc
    19561 gtgtaaatga ccaagataca gaatcccggc gtgaacgtgg atgagttgct gatgaagtga
    19621 gcgctttcac cctgccaaga atttatcggc aactaaaaaa cagtttttat gaatgactta
    19681 cgactttgct tatccgtgct tatcgttttg atacttgccg gctcgtgttc gaattacaaa
    19741 gaaggcaacc tccgtatctc gcctcaaaaa acagatcaaa gtgttaattt gggcnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgac gggtaccaaa
    19861 caacgaatgc actttcatct atttctgtaa cgatgagtat gaggtcattg gaagaaataa
    19921 aagcccgtat tactccgagc gccttgagag aaaagaagag cgttttgctg gagctgagcc
    19981 gggtaaatta tgatggggag gataacggtc tcttttctgt ggtgcatgat aaaatgacaa
    20041 acacgatcag gtatgcaatg gcaattagct acaatgacag ggtctattac atcaaggcct
    20101 tttgtttcga tcagcttgaa gaaagtttcg atccattgat aaggaaaagt tttatgagca
    20161 catatnnnnn nnnnnnnnnn nnnnnnnnnc agtatggaag agtttcggct tgctaagttt
    20221 gaaggtagaa accgatcaat tcaaactaaa gatggcgaat accctactca atctcctgat
    20281 gcagccgtta tagaatggac cactatggat gatctgacag gaatatcaga aaccggattg
    20341 gtcgaaaacg agcttcaaaa attgacgggt atggagcgcc cgggggtatc tgtgctctat
    20401 ttggagaatg gcaaatacta tcagggcacg gcaagtgcag aaaatataaa agctttgtag
    20461 gcttgctcgt tacggaaggc aggaagggaa ctttagtggt ttgtcatggt aatgccgaca
    20521 gcaaccttga agagtttgaa aagcatactt acggaaagtt ggtaaaaacc aagattgtgg
    20581 gacgcgctaa ttgagccttc acacccggtt ctcctgcgct ggcgagaacc gagcatcaaa
    20641 tacaaataaa aaagcctcgc aaatcaaaga tttacaaggc tttataagtg gtggagatag
    20701 tcggagtcga accgacgacc tcttgcatgc catgcaagcg ctctagccag ctgagctata
    20761 cccccctttt atccgccata gccaaagcga aggcgggact attttttagc ttttcttcaa
    20821 aagcggtcgc aaagttaaga caaaagagag aaaagtcaag agaaggcgaa gaaaaatatt
    20881 ttgaaaactt cacgaatgag atggctgatc cccctccccc gctgtatgcc gacgcttgaa
    20941 cggcaatatt tttttctgcc ataaaacaag agtatcgtac tttcgcaaag aaaagggttt
    21001 tatacaccaa atgagatttc aatgagcatt gcccttccca agcctgtcgg cgttgccata
    21061 gtggatgatc agcaattgtt tttagacggt atttcggcac tgttgaacgc ttgtccggag
    21121 ctggaagtcg tagctgccgc catgagcgga gcgcaggcat tgtctttatt tgagcaacga
    21181 tgcccggaaa tcgttttgct ggacctttcc atgcccgaaa gagatggcat ggaggtgctc
    21241 aacgacctca aggcgcattt cccggagata aaagttatca tgctcaccgt acacgacgat
    21301 ctgccgtcca tacaagagtg tctgcgcagg ggcgccatgg ggtatgtatt gaaaatcaat
    21361 ggtaaagatg agttgctaca cgctattctg gaggtggccg gcggcaggaa gtacttggat
    21421 cccaaagttt tggacacctt gataggccac gaaaagccgc ccgcaggcga cggtcagaag
    21481 accttgtcag gttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagct caacgcccgc aacatcaccg
    21661 acgccgtgcg catggcactg caaaaggggc tgatttagac ttcattccta aaaactgcct
    21721 cattggaaac gcgcaccact atggtagcgc catggccggg ttggctgtcg gtgttcattt
    21781 ggccgcccag caccgtcacg cgattgcgca cattggccag cccgatgccc tcgcccgccg
    21841 ttttccccga ctgaaacccc ttgccgttgt cttccacaat cagcacgatt tccttttcgt
    21901 gagccagcca ctgcacgctg agttgcgagg cttcagcgtg tttgagggcg ttgttgagcg
    21961 cttcctgggc aatccagtag agaccggttt ccgtttcagg ggtgagttgg agattggtag
    22021 ggg
//
LOCUS       DAORLF010000023        21487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1327_length_21487_cov_1.969345, whole genome shotgun sequence.
ACCESSION   DAORLF010000023 DAORLF010000000
VERSION     DAORLF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21487
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_21487_cov_1.969345"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK198_03610"
     CDS             complement(<1..231)
                     /locus_tag="PK198_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37852.1"
                     /translation="MLFKKTLHIAALLVVAYLMNSFVLRSGIMKDAPPRNDAIERRVD
                     SLLALMTLEEKIGQMTLFTSDWEMTGPTLRATY"
     assembly_gap    300..421
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<422..>653)
                     /locus_tag="PK198_03615"
     CDS             complement(<422..>653)
                     /locus_tag="PK198_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF37853.1"
                     /translation="WETYHDGLIGQYGFKDAFNPTFTFAPGTENGWFNKDYLGIDQGP
                     ILIQLENYRSGLIWKVMQKNPYIQEGLKKAGFK"
     assembly_gap    654..827
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<828..>1071)
                     /locus_tag="PK198_03620"
     CDS             complement(<828..>1071)
                     /locus_tag="PK198_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF37854.1"
                     /translation="EFEGQEMVNFSPLFGHQYSHMYVNFKGIQDDYMRSKGIDYFENA
                     RRATLANRAYCMRNPLGFKDYGENVWGLTACDGPAYL"
     assembly_gap    1072..1214
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1215..1718)
                     /locus_tag="PK198_03625"
     CDS             complement(<1215..1718)
                     /locus_tag="PK198_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HRF37855.1"
                     /translation="MPVAFSEDELQRRTFLYFWELADTVYWQIPDRWPTLTFSSIAAT
                     GFGLTTYIVGAERGFVTRQAAADRTVKTLRALWNLPQGPEASGVSGYQGFFYHFLNLD
                     DARRFKQVELSTIDTGLLMAGILSVQSYFDRKNPVETEIRQLADDLFRRVNWPWFLNK
                     NGLLSMGW"
     assembly_gap    1740..1790
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1961..>3736)
                     /locus_tag="PK198_03630"
     CDS             complement(1961..>3736)
                     /locus_tag="PK198_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37856.1"
                     /translation="DDIKRTPLPIPPPLEQFENGSFNGANLDWQPFNGDQAIHGTFAR
                     IANPDATGVNTTANVGRYQKGASPFSTLTAFLPNGLDLSSAPQLDLDVWAPAGAANVT
                     MQLVSASQGNKERTVAIPNTGQWVKLSFEYSDFADINDYEEVRLLFDNGVASSAIYYF
                     DNLAQNVSTVDPCAGTVPDLTILDDYECQRNITYGAGANRLEVVTNPALQVANASLKV
                     GKYTDPANEPWVALVFDNQAPIDLSTFNQFNIQVYAAQTGPMLVKLEGGTSPAREVWT
                     EVNVTNAWTNYVVDFSPFAGENHRKVAVFFNGGVGQPTEDIWYIDNVKWNRAPFRGCV
                     ATFDQPEYIVDNWRYFANGSLENTVFEAVANPNPSGINTSQNVGVFVEATDGEIFAGM
                     YADLPAPVALPVNNKKIRVKVLMDQAALVVFKLERGRDGAQNTGDVPMNPTTADNNYT
                     TPGQWQELTFDYSFLPDNTLHDRMTIIMNFRDVPTTVKTYYFDDITIGDNNCPGANFI
                     FEPVVEQLRIMPNPANDRLTVQNAEGMEHFVIHNAYGQVVKRVPNNGQYNFNIDLAGL
                     ANGMYVLTGYNAQGQLTANAKFIKQ"
     assembly_gap    3737..3746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3747..5623)
                     /locus_tag="PK198_03635"
     CDS             complement(<3747..5623)
                     /locus_tag="PK198_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37857.1"
                     /translation="MRKVLLLLVSAVFISLSAFGQAPYILYEDFEDGGIQAAWNAVNG
                     ATFAIVDNPDRNGNVTSKVASVTNSASDFNFMLVNTGSRINLTEYNQMRIKVWSPVAG
                     VQFLFKIEGPGPDIERFVPITTANQWVEYTIDFSNGAGITDLNTFLISFNPFNGPGDG
                     NTYYFDFIAASKPNSRTWETFESGTAALPWAGINGSYNGVVDNPDVSAGNMSAKVASY
                     TNIAPSDFCFALANTGAAFSLAEYNQFKFKAWSPIAPTRVLFKIEGGGQAVEKFIDIT
                     VANQWVEYTADLSGGAAYTGLTTFLISFNPFVAGGDNNTYYFDDIVAVRAERTYENFE
                     SPSGITWNAGNGAYNGPVANPDPASEYYNATATVGSYTNNPGSDFNFVIGELSAPIDL
                     STHNQFRVSIWSPTASKVLFKLEGNGQAVEATKNIVFDSIWVEYIFDFSSAVGLTNMN
                     KILIAVTPFEPGNGDTWYFDNVYAVPKANCSNASPDPKIIDDFDFKHNAAYGVGLATL
                     ALVNNPNPTPVNSSPKVGRFTDRPGAFNPLLIAYDDVLDLSTNNYISIKLWAPRPGDI
                     LFKLEGGASPAAEVRVPVTALNTWVEYGVDFSAQAGTSHKAIVLFFNAFDNTDQGGVY
                     F"
     gene            complement(5644..7545)
                     /locus_tag="PK198_03640"
     CDS             complement(5644..7545)
                     /locus_tag="PK198_03640"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRF37858.1"
                     /translation="MRLSVPKNREGSGSVMSMYWKTLLVVVLTAASASALRAQPVSPA
                     NLAGVGYDSHIELKWTASTSPNIIAYNIYRSASPDGPFALLRQVGASTPYALDFIGRI
                     DTVFSYRVTALNSSGQESAPTSVQTATVAAMSDDDLMTMVQRYTFRYFWEHSHPVSGL
                     ARERLNSGDIVTTGGSGFGISAIPVAVERGFITRQEGLLRMIQIVSFLELRAQRYHGA
                     FAHWLNGNTGATIPFSQFDNGGDLVETAFLMQGLLTVRSYFNQDVPLENSLRSTITRL
                     WEDVEWDWYRRNDSNVLYWHWSPNYQWQMNFQLRGFNETMITYILGVASPTHGIPASL
                     YHSGWASNGGFVTPAAYFGYPMYVGGFRGGPLFFAHYSYLGFDPRNKRDAYCNYFNRN
                     RNHTLINREYCIANPKNHAGYGPDSWGLTASDNPWGYLAHEPVFDRDNGTIAPTAALS
                     SMPYTPAQSMAALKHFYRNLGDRLWGYYGFTDAYNIGQNWFASSYLAIDQGPIIGMIE
                     NHRTGLLWDLFMANPEMQPALDSIGFIAEQSAVAPDLALPAEVLVYPNPNNGQFTLLM
                     EATESFTAQIQLMDAMGRQVRNWGATHIQNGESSLTLDTHELPAGVYQLWLSSGGRQH
                     TQRILVFHN"
     assembly_gap    7791..7985
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7986..>8206
                     /locus_tag="PK198_03645"
     CDS             <7986..>8206
                     /locus_tag="PK198_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37859.1"
                     /translation="NPKLRHAGLDLHRRTPLIPETLAENICRGDRVALARAITLLESS
                     LPEHRETVAQILEMCMPCRVPSFRVGITG"
     assembly_gap    8207..8379
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8380..9033
                     /locus_tag="PK198_03650"
     CDS             <8380..9033
                     /locus_tag="PK198_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRF37860.1"
                     /translation="FAFIRPSPAGESLGGVAKMTRDTILLCEAAGYDLILVETVGVGQ
                     SEMAVHSMTDFFLLLLLPGAGDELQGIKRGIVEMADLLVVNKADGERLPLARRARGEY
                     LNALHLFPPKENGWSPQVLTCSAAENTGIEEVWNSMQAFRSHTAANDSMERRRRQQAL
                     AAMEEQLQSALRELFLHHPVVRDQLPAIEQEVLSGRISPAKAAHILLTFFLNQNVSP"
     gene            9147..9737
                     /locus_tag="PK198_03655"
     CDS             9147..9737
                     /locus_tag="PK198_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF37861.1"
                     /translation="MRSLTIGIGLVVLAGFLFMKGCSSYNTFVDKEENVENAWSKVQS
                     AYQRRSDLIENLVATVKGAAQFEQKTLTDIANARANATSVKIDPSNLTPEKMEQFQQA
                     QSQLSQSLGRLLMVTENYPELKANQNFLELQSQLEGTENRVKVERDKFNDVVTDFNKS
                     VRRFPNSLFAGMFGFSQKAQFEAEQSAQSAPKVNFE"
     gene            9734..10165
                     /locus_tag="PK198_03660"
     CDS             9734..10165
                     /locus_tag="PK198_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF37862.1"
                     /translation="MTRLFSTEEEARIIDAIREAEQQTSGEVRVHVEKNLRGDAMKEA
                     IRTFRKLGMHRTAQRNGVLIFLAPERRELVIIGDEGIDAVVPAGFWDAERDLMLDHFR
                     RGEMCAGVCAAIAGVGEKLKAHFPFAKDDTNELSDEISYGN"
     gene            10249..>10721
                     /locus_tag="PK198_03665"
     CDS             10249..>10721
                     /locus_tag="PK198_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF37863.1"
                     /translation="MSLRLTVLLFLSVLSLSALQAKEPPARPNPPRLVNDFVGLLDGG
                     QADALERKLVAYNDSTSTQIAVVIESTIDGDDVFDYSYRLARAWGIGQKEKDNGILIF
                     VAFEDRKLYIVTGYGAEGFLPDAMAKRIIENIIKPAFREGRYYPGLDEAANIIMQ"
     assembly_gap    10722..10740
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10741..>11028
                     /locus_tag="PK198_03670"
     CDS             <10741..>11028
                     /locus_tag="PK198_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37864.1"
                     /translation="EPRKEGGGGFPWMTILIILALLIFLSMFMGQGGNHHRNDDDDDG
                     GYWRGGRYDMGPRPRGGWVIFPGGGGGGGGWNSGGGGGGFGGFGGGDFGGGG"
     assembly_gap    11029..11146
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11147..>11889)
                     /locus_tag="PK198_03675"
     CDS             complement(<11147..>11889)
                     /locus_tag="PK198_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF37865.1"
                     /translation="DGLADDKDDCPTQAGSQALKGCPDRDNDGIADKDDKCPDQAGPA
                     SNMGCPITDRDGDGIEDKDDACPDQKGPAATKGCPDRDGDGIADKDDECPDQKGLAAF
                     RGCPDRDGDGIADKADKCPDSPGPASNNGCPVISQEDKAVIDLAIKNVQFETGKTSLL
                     QTSFPILDQVAGLMKKYPDYKLRISGHTDSIGEAPANQTLSEGRAKTCYDYLVAKGVA
                     SGRMSHQGFGESKPIADNRYKAGRDQNRR"
     assembly_gap    11890..11948
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11949..>12231)
                     /locus_tag="PK198_03680"
     CDS             complement(<11949..>12231)
                     /locus_tag="PK198_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF37866.1"
                     /translation="FLYPYLYAGLGAVSQNWDDLYFSAPLGVGLNFRLANNAYFTTKA
                     EYRLGFKDLRNDVMAGFGLMITFGDAVDKTPAVSDRDGDGIPDAQDRCPD"
     assembly_gap    12232..12364
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12616..13551)
                     /gene="nusB"
                     /locus_tag="PK198_03685"
     CDS             complement(12616..13551)
                     /gene="nusB"
                     /locus_tag="PK198_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRF37867.1"
                     /translation="MLSRRNVRVKVMQALYAQSRDEQLSADDVMKLYRANIRHSFELY
                     LLNLLYVVKIAEYAEADHARRKAKLRPSDEDRAFRPLLSQNELVQSLTKHAEFQRALR
                     AHKLEKAADEDKVRQLYTELAKTPEYEKYLLLEAPTREDHIGILIHLYKMCLANEIFN
                     DTIEDSYPLWTDDESLIVGAMKKTIRALPAQPDFMEEFRPSREATVEFGEEMLRQLAR
                     NEQQYFGMIEPMLQNWDADRVAVVDMILLKMAICELLNFPTIPTKVTLNEFLEISKQY
                     STEKSKEFINGILDRMMKMLLKEGKIVKEGRGLDG"
     gene            complement(13596..14993)
                     /locus_tag="PK198_03690"
     CDS             complement(13596..14993)
                     /locus_tag="PK198_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37868.1"
                     /translation="MTILTLVLVIAIVAAVLTVVMKVVWPKRITTWWLSFLQNFCGGL
                     FLFSGFVKAVDPMGTAFKLEQYFAEFNATFSGAGWDGLAALFPWLSQFSVGFSVFMIV
                     FEMVLGVMLILGAMRGLTAWLFFLLVLFFTALTGFTYLTGYVPAGVNFFEFSKWGEFV
                     ETNMKVTDCGCFGDFIKLKPKTSFLKDVFLMVPAILFVLMFRRKHQLFSPKVRNIIVA
                     ASTVLLLVYNMNNYLWNEPHLDFRPFKVGVNIAQQKNLEMEAQNNVEITAYELTNKKS
                     GEVKTIAYDQFLKEYEKYSKEEWNFEQIQSEPFVWDTIRKGDDVKVEKRILKPSKISD
                     FNIMAGDDSDVTYEILEDQGYNFLVVGYKLYNKPEYLEKWKGKIATLAAAAAKDGVNL
                     RSVVAYTDAETLKSFGEVAGIGQYPLYKADDIMLKTIIRSNPGVLLLKNGKIVMKWHY
                     NKLPEYAQIKADYIR"
     gene            complement(15006..15551)
                     /locus_tag="PK198_03695"
     CDS             complement(15006..15551)
                     /locus_tag="PK198_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRF37869.1"
                     /translation="MQKTIEQFQAVFSRCKALFVNKNRDYGTSWRILRPASLTDQLFI
                     KARRIRTIEEKGYQKVADSVESEFIGIVNYCIMSLIQLQLPPDAPIEMDGQEVQVLYD
                     RYVRETLQLLESKNDDYGEVWRDMRSSSYTDLILVKLLRIKQIEDNEGRTLVSEGLDA
                     NYKDIINYAIFALIKIDEARQ"
     gene            15721..>18447
                     /locus_tag="PK198_03700"
     CDS             15721..>18447
                     /locus_tag="PK198_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF37870.1"
                     /translation="MRLLSFFAVLSLSALMLAMAGCNTSSRVSAGDKGGKAGFDIPYE
                     KYTLKNGLDVILHKDASDPIVAIAVLFHVGSNREVPGRTGFAHFFEHMLFQNSENVGK
                     GQFFKTINELGGDFNGGTSNDFTVYYETVPKDALEKILWMESDRMGFLINTVTVPVLE
                     NEKEVVKNEKRQRVDNVPYGHTSYVIDKAMYPADHPYNWQVIGSLEDLQAATIDDVKD
                     FYEKWYGPNNATMVIAGDIDIAQTKKWIDRYFAEIPAKAKVENLQPRLGKLDGVKRLM
                     HEDNFAQVPEMRMVWPTVQDGHPDSYALQYLGELLSDGKRAPLYKELVEKRKVAASPG
                     ASNRTLEIAGNFSIVVRANAGVDLDDAYAGTMDALKAFEQSEIDDKDMERIKNSLETN
                     FYNGLSSVFFKAFQLASANEYNGSPEALKTEIAKTLAVTKEDVKRVYDKYIKNQPYIL
                     TSFVPKGKPELAVANSAKAEVVEEPVVQGAEAAPMEETEAEFKRTPSKIDRTAIPPLG
                     EAPVINIPTVWSKDLSNSMKVLGIENAELPLVSFSLRIKGGMLQESPATIGVSNLLTD
                     LMMEGTKNKTPEELQDAIGQLGASISMSAGSEFISLSGNCLARNFDAVMALATEILLE
                     PRWDAAEFDRLKTAALNRIKQQAGQPNAISGNVFNKLMYGQSSILSYSPLGTTASVER
                     ITIDDLKMYYQKNFSPNLAAFHVVGAVNQAQVNSALMSIEQKWANKNAAVPAVPDARQ
                     ISKPVIYFVDIPGSKQSVVRLGAPLVKGNHPDYFAASVVNDKLGSGSGGILFQRLREE
                     RGYTYGANSSVPRRMSQGFFTAFSSVRANVTKESVQLFREIMQGYKDEYKQEYLDVTR
                     TAMLRSNALAFETLGNKMGILLNMSTFGLPADYVKQEEKQLMSMTLDQAKATI"
     assembly_gap    18448..18471
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18775..19551)
                     /locus_tag="PK198_03705"
     CDS             complement(18775..19551)
                     /locus_tag="PK198_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF37871.1"
                     /translation="MNIFIIEDEDMAARRLQKLISDCEPGAVISGRADGIESAVAWLK
                     NHPQPDLIFMDIHLSDGSAFEIFSRFQVQSPVIFSTAYDQYAIQAFKHHTIDYLLKPV
                     KVEELRQALAKYQRMTAAQTPDYQSLADLIPQVRAPRRFLIRSGSTMKVVDASEAAYY
                     FSQNKITYLVATNGKRYAIDFTMDKLEQLLDPTTFFRINRQFIISVAAIREMHAHSKS
                     RVKIVLSPSSEEEVVVSAERSPHFKRWLTNTDEDDFQADA"
     gene            complement(19548..>20760)
                     /locus_tag="PK198_03710"
     CDS             complement(19548..>20760)
                     /locus_tag="PK198_03710"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF37872.1"
                     /translation="RTDTAGMILLAHRNGLDLLDPAGTRFFHFEEAAGLTDFDPNLNA
                     STTDKSGQIWIGTSTGLWRYTPLGATRSAYPKTVLNQVSVSFQPVDFERKNKFSYNEN
                     NLVFSYSGIWHTDPERVRYRYRLSGFSRDWIYTADRQATFPNLPPGTYTFEVTAGMDE
                     VFEDTEVVSYVFVIRNPFWKEGWFLLPFVTALTALFIWWMRRRDERLRIEEARKKEQV
                     ENRLEVLKNQINPHFLFNSFNTLTSLIEEAPDLAVEYVEKLSDFFRNILQYREKDLIA
                     LEEELNVLDSYLFLLHKRYGDNLRVEITLRPTEGYIVPLALQLLVENAVKHNIVSASK
                     PLYMRIYAEGDYWMVENSLQPRISRPDSTGMGLKNIVKRYELLTEKEVRIEQTEHFFR
                     VGIPIISTEPS"
     assembly_gap    20761..20776
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20777..>21138)
                     /locus_tag="PK198_03715"
     CDS             complement(<20777..>21138)
                     /locus_tag="PK198_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF37873.1"
                     /translation="TLGGVSEYVLDADNPAQAPFMLRNYRQANGLGANFIYSAFTDSR
                     RRIWFGTDGQGISMLDKDRISQFRTAVTQSNDSLPLHAIYSFAEDRRGHIWFTTAKDG
                     LFSFDGRHFHHYKYADGL"
     assembly_gap    21139..21159
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21160..21469)
                     /locus_tag="PK198_03720"
     CDS             complement(<21160..21469)
                     /locus_tag="PK198_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF37874.1"
                     /translation="MSTPADNVQTVLADSRNRLWIGTPQGLWRRSLYGSHRAEWEQIS
                     ALKIFNIISLYEDAEANIWAGTFGQGLLFLPSGDGTPILLDEGQGLANNSVLSLTGTG
                     R"
BASE COUNT         4834 a         5207 c         5376 g         4812 t
ORIGIN      
        1 gtaggtggcg cgcaaggtcg ggccggtcat ttcccagtcg gaggtaaaaa gggtcatctg
       61 acctattttc tcctctaagg tcattaatgc cagcagcgag tccacccggc gttcgatggc
      121 gtcgttgcgc ggcggagcgt ctttcataat gccgctgcgc aatacgaaac tgttcatcag
      181 atatgccacc accaaaagtg cggcgatgtg tagtgttttt ttgaagagca tgatggtttg
      241 atattcaaag ttttaaattc aaagttttgg gttcaaggtt tcaggttcaa ggttccaagn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nccttaaacc ccgccttttt cagtccttcc tgtatgtacg gattcttttg cattaccttc
      481 cagatcagtc cgctgcgata attttccaat tgtatcaaaa tcggcccctg gtcaatgccg
      541 aggtaatctt tattgaacca gccgttttcg gttccaggag caaatgtgaa cgtcggattg
      601 aacgcatcct taaagccata ctgcccgatg aggccgtcgt ggtaagtttc ccannnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa ataagccggc
      841 ccgtcgcagg cggtgagacc ccatacgttt tcgccgtagt ctttgaaacc gaggggattg
      901 cgcatgcaat aagcccggtt agcaagtgtg gcccgacgtg cattttcaaa ataatcaatg
      961 cctttgcttc tcatgtaatc atcttgtatg cctttgaaat tgacatacat gtggctgtac
     1021 tgatgcccga acagcgggga aaaattgacc atttcctggc cctcaaattc cnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnccagcc catggagagc agaccgtttt tatttaaaaa ccacggccag
     1261 ttcacccgtc ggaacaggtc gtcggccaat tggcgaatct ctgtttccac aggatttttg
     1321 cggtcgaagt aagactgtac agaaaggatg cccgccatca gcagcccggt atctatggta
     1381 gaaagctcta cctgtttgaa ccggcgggca tcgtcgagat tcagaaaatg ataaaagaag
     1441 ccctgataac cgctcacgcc ggaggcttcc gggccttgcg gcaaattcca cagggcgcgc
     1501 agcgttttca cggtacggtc tgcggcggcc tgtcgcgtta cgaagccccg ctcggcgccc
     1561 acgatgtagg tcgtcaaccc aaatccggtg gcggcaatgc tggaaaaagt gagtgtaggc
     1621 cagcggtcgg ggatttgcca atacaccgta tcggccaatt cccaaaaata gagaaaagtg
     1681 cgccgttgca gctcgtcctc gctgaaagct accggcatgt tggagacggc aggctttgcn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaatagga
     1801 aaatggccca acgcatgatg tttggtttta tttggttgcc gagaaaagca caccgccagg
     1861 gcgcgaagcg agcaaaatgt gcttcgcgcc cctgtgcggt gttcaccaaa atgagcctaa
     1921 tgtttccggc ccaccaaggg agccggaagg ttttgtttgt ttactgtttg ataaacttgg
     1981 cgttggctgt caattgtcct tgtgcgttgt agccggtgag tacatacatg ccgttggcga
     2041 ggccagcgag gtcaatattg aaattgtact gaccattgtt cggtacgcgt ttcaccacct
     2101 ggccataagc gttgtggatc acaaaatgct ccattccttc ggcgttttgt accgtcaggc
     2161 ggtcgttcgc cggattgggc atgatgcgga gttgttctac gacaggttcg aagatgaagt
     2221 tggcgcccgg gcagttgttg tcaccaatcg taatgtcgtc gaaatagtac gttttcacgg
     2281 tagtgggcac atcgcggaag ttcatgatga tggtcatgcg gtcgtgcaac gtgttgtcgg
     2341 gcaggaagga ataatcaaac gtaagttcct gccattggcc cggcgtagtg tagttgttgt
     2401 cggctgtagt cggattcata ggcacgtcgc cggtattttg cgcgccgtcg cggccgcgtt
     2461 cgagtttgaa cacgacgagg gcagcctggt ccataagcac ttttacgcga atctttttat
     2521 tattcaccgg caatgccaca ggagcgggca ggtcggcata cataccggca aaaatttcgc
     2581 cgtcggttgc ctctacaaat acgcctacgt tctggctggt attgatgccg ctcggattgg
     2641 gattggcgac tgcttcaaaa acggtatttt ctaaagaacc gttggcaaaa tagcgccagt
     2701 tgtcgacgat gtattccggc tgatcaaatg tagcaacaca cccgcggaaa ggtgcacggt
     2761 tccacttcac gttgtcaatg taccagatgt cttcggtagg ctggcctacg ccgccattga
     2821 aaaatacggc gaccttgcgg tggttttcgc ctgcaaaggg gctgaagtcc actacatagt
     2881 tggtccaggc attggtaaca tttacttccg tccacacttc acgggcaggc gaggtaccgc
     2941 cttccagttt caccaacatc gggccggttt gagcggcata cacctgaata ttgaactggt
     3001 tgaacgtact tagatcaatg ggcgcttggt tgtcgaaaac cagagctacc cagggttcgt
     3061 tggccgggtc ggtgtatttg cccactttga gcgaggcatt ggccacctga agtgccgggt
     3121 tggtcactac ttccaaacgg ttggcgcctg cgccgtaggt gatgttgcgc tggcactcat
     3181 agtcgtccag aatggtcagg tcgggaactg taccggcgca gggatcaacg gtgctcacgt
     3241 tttgagccaa gttgtcgaaa tagtaaatgg cagaagacgc cacgccgttg tcgaacagca
     3301 ggcggacttc ttcgtagtcg ttgatgtcgg caaagtcgga gtattcaaaa ctgagtttca
     3361 cccactgacc ggtattcggg atggccacag tgcgctcttt gtttccttgc gaagcgctca
     3421 ccaactgcat cgttacattg gctgctccgg caggcgccca cacgtcgagg tcgagctgcg
     3481 gagcactgct caggtcaagg ccgttgggaa ggaatgcggt cagcgtactg aacggagaag
     3541 cgcctttttg atagcgcccc acattggctg ttgtgttcac gccggtagca tccgggttgg
     3601 cgatgcgggc aaaagtgccg tgaatggcct ggtcgccgtt gaagggctgc caatctaagt
     3661 tggcgccgtt aaaggaaccg ttttcaaact gctcgagtgg cggcgggatg ggcagcggcg
     3721 tgcgcttgat gtcgtcnnnn nnnnnnatga aatatacgcc gccctgatcg gtattgtcaa
     3781 aagcgttaaa gaatagcacg atggccttgt gactggtacc tgcctgtgca gaaaagtcca
     3841 cgccgtattc cacccaggta ttgagagcgg ttaccggtac gcgcacttca gcagcaggcg
     3901 aagcgccgcc ttcgagtttg aagaggatat ctccggggcg cggcgcccag agtttgatgc
     3961 tgatatagtt attggtactc agatcaagga cgtcgtcgta ggcaatcaat aaaggattga
     4021 aagcgcccgg acggtctgtg aaacggccca ccttcgggct ggagttgacc ggtgtggggt
     4081 tgggattgtt gaccaaagca agcgtagcaa gcccgacacc gtatgccgca ttgtgcttga
     4141 agtcaaaatc gtctatgatt ttcggatcag gcgaagcgtt ggagcaattg gctttgggca
     4201 ccgcatatac gttgtcgaaa taccaggtgt cgccattgcc cggctcaaag ggagtgactg
     4261 cgattaaaat tttattcata ttcgtcagtc ctaccgctga agaaaaatca aaaatgtatt
     4321 cgacccatat gctgtcgaac acaatatttt ttgtggcttc cacggcttgc ccgttgcctt
     4381 cgagtttgaa aagcactttg ctggcagtag gcgaccaaat actgacccgg aattgattgt
     4441 gcgtgctcaa gtcgatagga gcgctcaact ctccgatcac gaagttaaag tcggagccgg
     4501 ggttgttggt atatgaacca actgtagcag tggcgttata atattcacta gccggatccg
     4561 gattggctac cgggccattg taggcgccgt ttcctgcatt ccaggtaatt ccggagggag
     4621 attcgaagtt ttcgtaggtt ctttcagccc gtaccgccac gatgtcgtca aaataatagg
     4681 tattgttatc accacctgcc acaaacggat tgaacgaaat caggaaagtc gtcagcccgg
     4741 tatatgcagc gccccccgag aggtctgccg tatattccac ccattggttg gctacagtga
     4801 tgtcaatgaa tttctctaca gcctggcctc cgccttcaat tttgaagagt actctggtag
     4861 gagcaatggg cgaccaggcc ttgaacttaa actggttgta ctcggcaagg ctgaatgcgg
     4921 cgccggtgtt ggccaacgcg aaacagaagt cggaaggcgc aatattggtg taggaagcca
     4981 ctttggcgct catattgccg gcgctcacgt cgggattgtc cactacgcca ttgtaagagc
     5041 cgttgattcc tgcccatgga agggctgctg tcccggattc aaaggtttcc caggtcctgc
     5101 tgttgggttt ggatgccgca atgaagtcga aatagtaagt attgccgtca ccagggccat
     5161 tgaacgggtt gaaggaaatc aggaaagtat tcaggtcggt aatacctgcg ccgttcgaga
     5221 agtcaatggt atattccacc cactgattgg cggtggtgat gggcacaaaa cgttcaatat
     5281 cgggccctgg cccttcgatc ttgaacaaaa actgaacgcc tgccaccggc gaccatacct
     5341 tgatgcgcat ttggttgtac tccgtcaggt tgatccggct gccggtattc accagcatga
     5401 agttgaaatc ggatgcgctg ttcgtcaccg atgccacttt actggtgaca tttccattcc
     5461 gatccgggtt gtcaacaatg gcaaaagtgg cgccattaac ggcgttccag gcagcctgga
     5521 tgccgccatc ttcgaagtct tcgtacaaga tgtacggggc ctgcccgaat gcggagaggc
     5581 tgatgaagac cgctgagaca agaagaagta agacttttct catggtttta cttgttttta
     5641 aaattaatta tgaaagacta aaattctctg cgtgtgctgg cgcccgcctg aagaaagcca
     5701 aagctgataa actcctgcgg gaagctcatg ggtgtctaaa gtcaggctgc tttctccatt
     5761 ttgaatatga gtggcccccc agttgcgcac ctgtcggccc attgcgtcca ttagttgaat
     5821 ttgcgcagtg aaagactcgg ttgcttccat caataaagtg aattgcccat tgttaggatt
     5881 aggatagacc aacacttcgg cgggcaaagc caaatcggga gccacagcac tttgttcggc
     5941 aataaatccg atggaatcca atgcgggctg catttcaggg ttggccatga acaaatccca
     6001 cagcaaaccc gtgcgatggt tttctatcat accgatgatc ggcccctgat ctatggctaa
     6061 ataagagctt gcaaaccagt tttggccgat attatatgcg tctgtaaatc catagtaccc
     6121 ccacaatctg tcccccagat tgcgataaaa atgcttgagc gccgccatgg actgcgccgg
     6181 cgtatatggc atggaagaaa gagcagcggt aggagcaatg gtgccgttat cgcggtcaaa
     6241 aacaggctca tgagctaagt agccccaagg gttgtcgctc gcggtcagtc cccaggaatc
     6301 ggggccgtag cccgcatgat ttttggggtt ggcaatgcag tattccctgt tgatgagcgt
     6361 gtggttgcga ttgcggttga agtagttgca gtaagcatcg cgtttgttgc gcgggtcaaa
     6421 accgaggtaa gaatagtgcg caaagaacag cggcccgccc cggaaaccgc ccacatacat
     6481 ggggtagccg aaatatgccg ccggcgtcac aaagcctccg ttgcttgccc agccgctgtg
     6541 gtacaagctc gccgggatgc cgtgcgtagg cgaagcaaca cctaatatat aggtaatcat
     6601 ggtttcgtta aatccgcgca actgaaaatt catttgccac tggtagttgg gcgaccaatg
     6661 ccagtacagc acgttactgt catttcggcg ataccagtcc cattctacat cttcccaaag
     6721 ccgggtaatg gtactgcgca gggagttttc caaaggtacg tcctgattga aatacgaccg
     6781 caccgtgagc aagccctgca tcaggaaagc ggtttccacc aaatcgccgc cattgtcaaa
     6841 ctggctgaat ggaatggtag ctcctgtatt tccattgagc cagtgcgcaa aagcgccatg
     6901 atagcgctgc gccctcaatt ccagaaacga aacgatctgt atcatgcgca gcaagccctc
     6961 ttgccgggtg atgaagcccc gctcaacggc taccggaata gctgaaatgc caaaaccgga
     7021 tcctcccgtt gtgacgatat ctccagaatt gagcctttcg cgtgccaaac ctgagactgg
     7081 atgggagtgc tcccaaaagt atcgaaaagt gtagcgttgt accatcgtca tcaggtcatc
     7141 gtcgctcatg gcggccaccg tggcggtttg cacactcgtt ggggcgcttt cctgcccgga
     7201 gctattgagc gcagtgacgc gatacgaaaa cacagtatct attcggccaa taaaatcgag
     7261 ggcataaggc gtactcgcac ctacctgacg cagcaatgca aacgggccat ccggcgaagc
     7321 agaacgatat atattatagg cgatgatgtt gggggaagtc gaggccgtcc atttcagctc
     7381 tatgtggctg tcataaccga cgcctgctaa attggccggc gagacaggct gtgcccgcaa
     7441 tgccgacgcc gaagcagccg tcaatacaac tacgagaagt gttttccaat acatagacat
     7501 cacacttccc gacccttctc tgttttttgg tacggataac ctcatgagca ttcaaaaatt
     7561 ctttgtgata gcgagtgtaa aattacccct attcaaggta agtaccaaat ttttaacctc
     7621 actattgcta cgccaatgca attcaagagc ataacacaaa caatcatact taattgtatt
     7681 tcaaatactt atttaataaa tgacaaaaat cactactacg ccatgatgct attttgacct
     7741 aagttggaag ggtatagcga ggcccgcgaa ggaacgcgcg tgtacgggcg nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnncaatc cgaaactgcg ccatgccggt ctcgatcttc accgacgtac gcctttaatt
     8041 ccggaaacac tggctgaaaa catctgccgg ggcgaccgcg tagcactggc ccgcgccatc
     8101 accctcctcg aaagcagcct gcccgaacac agagaaacag ttgctcaaat attggaaatg
     8161 tgcatgccct gccgcgttcc ttcgttccga gtgggcatca caggttnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttgccttcat ccgcccttct
     8401 cctgcgggcg aatccctggg cggcgtagcc aaaatgaccc gcgataccat actgctgtgc
     8461 gaggctgccg gctacgacct cattttggtg gaaaccgtag gcgtgggcca atcggaaatg
     8521 gccgtacatt ccatgaccga ttttttccta ctcttgctgc tgcccggcgc cggcgacgaa
     8581 ttgcagggca tcaaacgcgg cattgtagaa atggccgatc ttttggtggt caacaaagcc
     8641 gacggcgaac gcctgccttt agcccgccgt gccagaggcg agtatctcaa cgccttgcac
     8701 ctctttccgc ccaaagaaaa cggttggtcg ccgcaagtac tcacctgctc cgccgccgaa
     8761 aacacgggga ttgaagaggt ttggaactcc atgcaggcat tcagatcaca caccgccgcc
     8821 aacgactcaa tggagcgacg ccgccgccaa caggcactcg ccgctatgga ggagcaattg
     8881 caaagcgcgc tccgagagct ttttttgcac cacccggtcg ttcgggatca actgccggcc
     8941 atcgagcagg aagttttaag cgggcgcata tcgccggcga aggcagcgca tatattgctg
     9001 acattttttt tgaaccaaaa tgtatctcca taaaccgtat ttcgtctaag ttgcgccgtt
     9061 gttgagcgtc ggcaatatgg cgttcggcgt tcatcgccat ctttttccga gcatcattgt
     9121 ttcattcatc aaaaacaagc aaccttatga gatcacttac catcggcatc ggactcgttg
     9181 tgctcgccgg ttttctgttt atgaaaggct gttcttccta caacaccttt gttgacaagg
     9241 aagagaatgt agaaaacgcc tggagcaaag tacagagcgc ttatcaacgc cgctccgatc
     9301 ttattgaaaa tctggtggcc accgtcaaag gcgccgctca gtttgaacaa aaaacgctga
     9361 ccgacattgc caacgcccgc gccaatgcta cgagtgtcaa gattgatccg agcaacctga
     9421 cgccggaaaa aatggagcaa ttccagcagg cgcaatcgca gttgtcgcaa agcttaggcc
     9481 gcctgttgat ggtgacggaa aattatcccg aactgaaggc caaccagaac tttctggagt
     9541 tgcaatcgca attggaaggc accgagaacc gcgtaaaagt agagcgcgac aaattcaatg
     9601 acgtggttac cgacttcaac aaaagcgtgc gccgtttccc caacagcctc tttgccggca
     9661 tgttcggttt ttcacaaaag gctcagtttg aggcggaaca aagcgcgcaa agcgctccga
     9721 aagtcaattt tgaatgaccc ggctgttcag caccgaagaa gaagcgcgca tcattgacgc
     9781 cattcgcgag gccgagcaac aaacctccgg cgaggtgcgg gtgcatgtgg aaaaaaacct
     9841 gcgcggcgat gctatgaagg aagccatacg taccttccgc aagctcggta tgcaccgcac
     9901 ggcccaacgc aacggcgtgc tgattttttt ggcgcccgaa cgccgggaat tggtcatcat
     9961 cggcgatgaa ggtatagatg ccgtggtgcc cgcaggcttt tgggatgccg agcgcgatct
    10021 gatgctcgac cattttcgcc ggggtgagat gtgcgccgga gtctgcgccg caattgccgg
    10081 cgtaggcgaa aaattgaaag cgcattttcc tttcgcaaag gatgatacca acgaactttc
    10141 ggacgagatt tcttacggaa actaatgaaa agcgccgcag atcgctcgcc ggtctgtggc
    10201 gctttatctg cacaaaaaac gaatgcccca acacaaaaaa aacacaccat gtcactccgc
    10261 cttacggttt tgctcttttt gtcggtactc agcctgagcg cgcttcaggc caaagaaccg
    10321 cctgcgcgcc ccaatccgcc gcggttggtc aatgactttg tcgggctgct cgacggcggc
    10381 caggctgatg cgctggaacg caaactcgtt gcctacaacg actccacctc cacacagata
    10441 gcagtggtca ttgaatccac gattgacggc gacgacgtgt tcgattattc gtaccgcctg
    10501 gcgagggcat ggggcatagg tcaaaaagaa aaagacaacg gcattctcat ttttgtggcg
    10561 tttgaagacc gcaaactcta catcgtcacg ggctacgggg ccgagggttt cctgcccgat
    10621 gccatggcca aacgcatcat cgaaaacatc atcaagcccg ctttccggga aggacgctac
    10681 taccccggcc tcgacgaagc tgccaacatc attatgcaat tnnnnnnnnn nnnnnnnnnn
    10741 gagccgcgca aggaaggtgg cggcggcttt ccctggatga ccattctcat catactggcg
    10801 ctgctcattt tcctgtccat gtttatgggt cagggcggca accatcaccg caatgacgat
    10861 gacgacgacg gcggttactg gcgcggcggc cgctacgaca tggggccgag accacgaggc
    10921 ggctgggtca tatttcccgg cggcggtggc ggcggcggtg gctggaacag cggtggtgga
    10981 ggcggcggat tcggagggtt cggcggcggc gattttggcg gcggcgggnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg gcggttttgg
    11161 tcgcggcctg ctttgtagcg attatcagct atcggtttag attcaccgaa gccctggtga
    11221 gacatgcgtc ctgatgcaac gcctttagcc accaaatagt cgtagcaagt ttttgcgcgg
    11281 ccttcagaga gcgtctggtt ggcgggggct tcgccgatgc tgtctgtatg cccgctgatg
    11341 cggagtttgt aatcaggata ttttttcatc agaccggcca cctgatcgag gatcgggaag
    11401 gaagtctgaa gcaggctggt tttgccggtt tcaaactgca cgtttttgat ggcgaggtca
    11461 atcacggctt tgtcttcctg agaaatcaca gggcagccgt tgttggaagc cgggccggga
    11521 gaatccgggc acttgtcggc cttatctgca atgccgtcgc cgtcgcggtc ggggcaacct
    11581 ctgaaagcag ccaaaccttt ttgatcggga cactcatcgt ctttgtcggc tatgccgtcg
    11641 ccatcgcggt cggggcagcc tttggtagca gccggacctt tctgatcggg gcaggcatcg
    11701 tctttgtctt caatgccgtc gccgtcgcgg tcggtgatcg ggcaacccat attggatgcc
    11761 gggcctgcct ggtcgggaca cttgtcatct ttatcggcta tgccgtcgtt gtcgcggtcg
    11821 gggcagccct tgagtgcctg cgagcctgcc tgagtggggc agtcgtcttt atcgtcagct
    11881 aagccgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnncg tcaggacagc ggtcttgcgc gtcgggaatg ccgtcgccgt cgcggtctga
    12001 aacagcgggc gttttatcaa cagcgtctcc aaatgtaatc atcaggccga agcccgccat
    12061 tacatcgttg cgcaggtctt taaaacccag gcggtactct gctttggttg taaaataagc
    12121 gttattggca agcctgaagt tcagccccac gcccagcgga gcagagaagt agagatcatc
    12181 ccaattttga cttacagcgc ccaggccggc atacaaataa ggataaagaa annnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnacattg cccagagaat gaatgtattc cacctccgcg ccggtgcgaa aatccgccct
    12421 gagcatttta tcagtgagtg gaaattgaaa gttggtgcgg gttaatctag ctgcgaaccc
    12481 gctgggagca gtttgccccc aaattgccgt ttggcctaag agcatggcca aggcaaagaa
    12541 gataatcgtc ctcttcatgt tgttttgttt tgtttgaata agaaaaaagg cgcttgaagt
    12601 tacgaaagtt ttcctttaac catccaagcc tctgccttct ttgacgatct taccttcttt
    12661 aagtaacatt ttcatcatgc ggtcgaggat gccgttgatg aactctttgc tcttctcggt
    12721 gctgtattgt ttagaaattt cgaggaattc gttgagcgtc actttggtgg gaatagtggg
    12781 gaaattcaac aattcgcaga tggccatttt gagcaaaatc atgtccacta cggctacgcg
    12841 gtcggcatcc cagttttgca acatgggttc tatcatgccg aaatactgct gctcatttcg
    12901 ggcgagttgc cggagcattt cttccccaaa ctcaacggta gcttcccgcg aagggcgaaa
    12961 ctcctccatg aaatcaggct gggcaggcag tgcgcgaatc gtttttttca tggcgcctac
    13021 aatcaacgat tcatcgtcgg tccacagcgg gtacgagtct tcaatggtgt cgttgaagat
    13081 ttcgttggcc aagcacattt tgtacaaatg gatcagtatg ccgatgtggt cttcgcgagt
    13141 gggggcctcc agcaacaggt atttttcgta ttcgggggtt ttggccagtt ctgtgtatag
    13201 ctgccgcacc ttgtcttcat cggccgcttt ttccagtttg tgcgcccgca gcgcccgctg
    13261 aaattcagca tgtttggtca acgactgcac caattcgttt tggctgagca gtggcctgaa
    13321 tgcgcggtct tcatcgctgg ggcgcaattt ggctttgcgg cgcgcgtggt cggcctctgc
    13381 gtactcggca atttttacca catacagcag gttgagcagg tacaactcga aggaatgtcg
    13441 aatattagct ctgtacaatt tcattacatc atctgcgctg agctgttcat cccggctttg
    13501 ggcgtacagc gcctgcatca cttttacacg gacattcctt ctgcttaaca tacaaatttg
    13561 gataagaact ttagaggcgg caaagatgct gtttattatc gaatatagtc ggcctttatt
    13621 tgtgcgtatt ccggcaattt attgtagtgc catttcatca caattttgcc gtttttgagc
    13681 agtaaaaccc ccggattgga acgaatgatg gttttgagca tgatgtcgtc ggccttgtac
    13741 aaggggtatt gcccgatgcc ggcgacttca ccaaaggatt tcagcgtttc agcatcggta
    13801 taagctacca cagagcgcag attgacgccg tctttggcag ccgcggcggc cagcgtggcg
    13861 atcttgcctt tccatttttc caaatattca ggtttgttgt agagcttgta gcccaccacg
    13921 aggaagttgt aaccctggtc ttcgaggatt tcgtacgtca catccgagtc gtcgccggcc
    13981 atgatgttga aatcgctgat tttgctgggc ttaaggatgc gtttttctac tttcacatcg
    14041 tcgcccttgc ggatggtatc ccacacaaaa ggctcagact gaatctgttc aaaattccat
    14101 tcttctttgg agtacttttc gtactctttc aaaaactggt cgtaagcgat ggtttttacc
    14161 tcgccgcttt ttttgttggt cagctcgtac gctgtgattt ccacgttatt ttgggcttcc
    14221 atttccagat ttttctgctg tgcgatgttg acgcccactt tgaacggccg gaaatcaaga
    14281 tgcggttcgt tccagaggta gttgttcatg ttgtacacca acaacaacac ggtggaggcc
    14341 gccacgatga tgttgcgcac tttggggctg aacaactggt gtttgcggcg gaacatgagt
    14401 acaaacaaaa tggccggcac catcaaaaag acgtctttga ggaaggaggt tttgggcttg
    14461 agtttgatga aatcgccgaa gcaaccgcaa tcggtcactt tcatgttggt ttccacaaat
    14521 tcgccccact tgctgaattc aaagaagttg acgcccgccg gtacatagcc cgtcaggtag
    14581 gtaaagccgg tcagcgccgt gaagaacagc accaacagga aaaacaacca ggccgtaagt
    14641 ccgcgcatgg ccccgaggat cagcataacg cccagcacca tctcaaacac gatcataaac
    14701 actgaaaacc ctactgaaaa ctgcgacagc caagggaaca gtgccgccaa accatcccag
    14761 cctgcgccgg aaaacgtggc gttgaattca gcaaaatact gctccaattt gaaagcagta
    14821 cccatgggat ccacagcttt tacaaagccg gaaaagagga acagcccgcc gcagaagttt
    14881 tgcaaaaaac tgagccacca ggtggtgatg cgcttgggcc acactacctt cattacaacg
    14941 gtgagcacgg cggcgacaat ggcaataacg agtacgagcg tgagaatggt catgattgaa
    15001 atgatttatt gtctggcttc atctatcttg atgagcgcaa aaatagcata gttgatgata
    15061 tctttataat tggcgtctaa gccttccgat acaagggtgc ggccctcgtt gtcttcgatc
    15121 tgtttgatgc gcaggagttt caccaaaatc aggtcggtat aggaagagga acgcatatcg
    15181 cgccacactt cgccatagtc gtcgtttttc gattcgagca gttgcaaggt ttcccgtaca
    15241 tagcggtcgt agagaacctg cacttcctgc ccgtccattt cgatgggggc atcgggcggc
    15301 agttgcaact gaatgagcga catgatgcag tagttgacga tgccgatgaa ctcggattcg
    15361 acggagtctg ctactttttg gtagcctttt tcctcaatgg tgcgtatgcg gcgggctttg
    15421 atgaaaagct ggtcggtgag gctggccggg cgcaggatac gccaggatgt gccgtagtct
    15481 ctgttcttgt tgacaaacag cgctttgcat cttgaaaaaa cagcctgaaa ttgctcgatg
    15541 gtcttttgca cgaggcgaat tttgcgacaa agatagggtt ttggtgtaat ttttaataat
    15601 ggttcgccct cagtcgtctg cccgacaacc tgcgtttcac atattcgccg taagtttgtt
    15661 gccttattga tcatttaccc ggaagggaat catccattca tccaccaaac aactgttatt
    15721 atgcgattgc tctcattttt tgcagtgctg tccctttcag cgctcatgct tgctatggca
    15781 ggctgcaaca cctcctcgcg ggtaagcgcg ggagacaaag gcgggaaagc cggtttcgac
    15841 atcccgtatg aaaaatacac cctcaaaaac ggcctcgacg tcattctgca caaagacgcc
    15901 tccgatccca ttgtggcgat cgccgtgctg tttcatgtcg gttccaaccg cgaagtgccc
    15961 ggccgcaccg gctttgcgca cttcttcgaa cacatgttgt ttcaaaattc tgaaaacgta
    16021 ggcaaaggcc agtttttcaa aacgatcaac gaactcggcg gcgatttcaa cggcggcacc
    16081 tccaacgact tcaccgttta ttacgaaacc gtgcccaaag atgccctcga aaaaatcctc
    16141 tggatggaga gcgaccgcat gggcttcctt atcaataccg taacggtgcc ggtgttggaa
    16201 aacgaaaagg aagtggtgaa aaacgaaaaa cgccagcgcg tggacaacgt gccctacggc
    16261 cacacttctt acgtcataga caaggccatg taccccgccg accaccccta caactggcag
    16321 gtcatcggca gcctggaaga ccttcaggcc gctacaattg acgatgtgaa ggatttttat
    16381 gaaaaatggt acggccccaa caacgccacc atggtcattg ccggcgatat tgacattgcc
    16441 caaaccaaaa agtggataga ccgctacttc gctgaaattc cggccaaagc caaagtggaa
    16501 aacctccagc cacgcctcgg caaattggac ggtgtgaagc gcctcatgca cgaagacaat
    16561 tttgcccagg tgcccgaaat gcgcatggtt tggcccaccg tacaggacgg ccaccccgac
    16621 tcgtatgcgc ttcagtacct cggcgaactg ctctccgacg gcaaacgcgc tcctttgtac
    16681 aaagagttgg tagaaaaacg caaagtggcc gcttcgccgg gcgcctccaa ccgcaccctc
    16741 gaaattgcag gcaatttcag cattgtggta agggccaatg caggcgtgga cctcgacgat
    16801 gcttatgccg gcaccatgga cgcgctcaag gcttttgaac aaagtgaaat tgacgacaaa
    16861 gacatggagc gcattaaaaa ctcattggaa actaattttt acaacggtct ttccagtgtg
    16921 tttttcaaag ccttccagtt ggcttccgcc aatgaataca acggcagccc cgaagccctc
    16981 aaaaccgaaa ttgccaaaac actggctgtc accaaagagg atgtgaagcg cgtttatgac
    17041 aaatacatca aaaaccagcc ctacattctc accagttttg tacccaaagg caagccggaa
    17101 ttagccgttg ccaactccgc caaagctgaa gtagtggaag aacccgtcgt gcagggcgca
    17161 gaagccgcgc cgatggaaga aactgaggca gaatttaagc gcacgccctc caaaattgat
    17221 cgcacggcaa ttccgcccct cggcgaagcg ccggttatca atatcccaac ggtatggagc
    17281 aaagacttgt ccaactccat gaaagtactg ggcatcgaaa acgccgaact gccattggtc
    17341 agcttcagct tgcgcatcaa aggcggcatg ttgcaggagt ctcccgctac catcggcgta
    17401 tccaacctgc tgaccgacct gatgatggaa ggcacgaaaa acaaaacgcc ggaagaactg
    17461 caagacgcca tcgggcagtt gggcgcatct atcagtatga gcgccggctc ggaattcatc
    17521 agcctgtcgg gcaattgctt agcgcgcaat tttgatgcag tgatggcttt ggccaccgag
    17581 attttgctgg aaccgcgctg ggatgccgcc gaattcgacc gcctgaaaac agccgctctc
    17641 aaccgcatca aacaacaggc cggccagccc aacgccattt ccggcaatgt tttcaacaag
    17701 ctcatgtatg gccaaagcag catcctttct tattctccgc tcggcacaac cgcatcggta
    17761 gagcgcatca ccatagacga tctgaaaatg tactaccaaa agaatttcag ccccaatctg
    17821 gctgcatttc atgtggtagg agccgtaaac caggcgcagg tcaacagtgc attgatgagc
    17881 attgagcaaa aatgggccaa taaaaacgct gcggtgcctg ctgttccaga tgcccgccag
    17941 atcagcaaac cggttattta ttttgtggac atacccggtt ccaagcagtc ggtggttcgt
    18001 ttgggcgcgc ctttggttaa aggcaaccac cccgactatt tcgctgcctc ggtggtgaac
    18061 gacaaattgg gatcaggctc gggcggcatt ttgttccagc gcctccgcga agaacgcggc
    18121 tatacctacg gcgccaattc cagcgtgccg cgccgcatga gtcagggatt tttcactgct
    18181 ttctccagcg tgcgcgccaa tgtgaccaaa gaatcggtgc agttgttccg cgaaatcatg
    18241 cagggctaca aagatgaata caaacaggaa tacttagacg taacgcgcac cgccatgctg
    18301 cgcagcaatg cactggcctt tgaaacgctg ggcaacaaaa tgggtattct actgaatatg
    18361 agcaccttcg ggctgccggc agactacgtc aaacaggagg aaaaacaact gatgtccatg
    18421 acgctcgatc aggcgaaagc caccatcnnn nnnnnnnnnn nnnnnnnnnn ncatgaaccc
    18481 caacaagatg ttttacctca tcgtgggcga tgctgctaca caggcgccgc tgctcaaaga
    18541 gcttggtttt gccgatgtca tcatgttgga ccgcgacggc aacgtggtga ataactaatc
    18601 gcgacaagca attgggttca cccctgttta gttttgggcg aacaccacga tgaatttggc
    18661 tacaagtagc tgtttatgaa acatataggc acatagagca catagtacca accctatgtg
    18721 aactatgtgc ctattgtgtt ttaaaacaaa tggcgcggga tgccgccctc tcattcaggc
    18781 atctgcctga aaatcatctt cgtccgtatt ggtcagccag cgtttgaaat gcggcgaccg
    18841 ctcagcactc accaccacct cttcttcgga agacggcgac aacacgatct tgaccctgct
    18901 cttggaatgt gcgtgcattt cgcgaatggc cgccacggaa atgatgaact gccggttgat
    18961 gcgaaaaaat gtagtgggat cgagcagttg ctctaattta tccatcgtaa aatcaatggc
    19021 atagcgcttt ccgtttgtgg ctaccagata ggtgattttg ttttgcgaaa aataatacgc
    19081 cgcctcggaa gcatccacca ctttcatcgt agaaccgctg cggatgagaa accggcgcgg
    19141 cgcccgaacc tgtggaatta aatcggcaag cgactgataa tccggggttt gcgctgccgt
    19201 catgcgttgg tatttggcca aagcctgccg aagttcttcc actttgacgg gcttgagcag
    19261 gtagtctatg gtgtgatgtt tgaaagcctg aatggcgtac tgatcgtaag ctgtggaaaa
    19321 aatgaccggg ctttgcacct gaaatctgga aaaaatctcg aacgccgagc cgtcgctcag
    19381 gtggatgtcc atgaaaatca aatcgggttg gggatgattt ttcaaccagg ccacagccga
    19441 ttcaatcccg tcggcacggc ccgaaataac agcgcccggc tcgcagtctg aaatgagttt
    19501 ttggaggcgg cgggcggcca tgtcttcgtc ttcgatgatg aaaatattca tgacggttcg
    19561 gttgaaatga tgggaatacc aacgcgaaaa aagtgctcgg tttgttcgat gcgaacttct
    19621 ttctctgtga gcaattcgta gcgttttacg atgtttttca aacccatgcc ggtcgaatcg
    19681 gggcggctga tgcggggttg caggctgttt tcgaccatcc aataatctcc ctcggcatag
    19741 atgcgcatgt acagtggttt ggaggcagaa acgatgttgt gcttgacggc gttttccacc
    19801 agcaattgca gggccagcgg cacaatgtac ccttccgtgg gtcgaagcgt tatttccacc
    19861 cggaggttgt cgccgtagcg tttgtgcagc aaaaagaggt agctgtccaa cacatttaat
    19921 tcttcctcca gcgcaatgag gtctttctct ctgtattgca ggatattgcg aaagaaatcc
    19981 gagagttttt ccacatactc gacggccaaa tccggcgctt cctcaatgag cgaggtaagg
    20041 gtgttgaaac tgttgaacag aaaatgcgga ttgatctgat ttttcaacac ttctaagcga
    20101 ttttccactt gctctttttt gcgggcttcc tctatgcgca agcgttcgtc gcggcggcgc
    20161 atccaccaga taaacaaggc cgtaagcgcc gtaacaaaag gcagtaaaaa ccagccctct
    20221 ttccaaaacg gattgcggat gacgaagaca taactaacaa cttcagtgtc ttcaaaaacc
    20281 tcatccatgc cggcagtcac ctcgaaggtg taagttccgg gcggcaggtt ggggaaagtg
    20341 gcctggcggt cggcggtgta aatccaatcg cggctgaacc ccgacaagcg ataccgatac
    20401 cgcacgcgtt cggggtcggt gtgccagatg ccggagtagc tgaataccaa gttgttttcg
    20461 ttgtaagaaa atttgttttt gcgctcaaaa tcaacaggtt gaaatgacac agacacctga
    20521 ttgagcactg ttttcggata ggccgagcga gtggcgccga ggggagtgta tcgccagagg
    20581 cccgtggaag tgccgatcca gatttggccg cttttgtcgg tagtggaggc gttcaaattg
    20641 gggtcaaaat cggtgaggcc ggctgcttct tcaaagtgaa aaaagcgggt gccggcggga
    20701 tccagcaggt ctaagccgtt gcgatgcgcc agcaaaatca tgcccgccgt gtcggttctg
    20761 nnnnnnnnnn nnnnnngagg ccgtcggcat atttataatg atgaaagtgc cgcccgtcga
    20821 acgagaacag gccgtctttg gcggtggtga accatatatg gccgcggcgg tcttcggcaa
    20881 aagagtaaat ggcgtgcaga ggcaggctgt cattggactg cgtcactgcc gttctgaact
    20941 gcgatatgcg gtctttgtcc aacatactga tgccctgccc gtcggtgccg aaccaaatcc
    21001 ggcggcggga atcggtgaaa gccgagtaaa taaaattggc gcccaagcca ttggcctgcc
    21061 ggtaattgcg caacatgaag ggcgcctgag ccgggttatc ggcatccaaa acatattctg
    21121 aaaccccgcc caatgtagnn nnnnnnnnnn nnnnnnnnng ccggccggtt cctgtcagac
    21181 tcaaaacgct gttgttggcc aagccttgcc cttcgtccaa taagatcggt gtcccatcgc
    21241 cggaaggcaa aaaaagcagt ccctgaccaa aggtacccgc ccagatattg gcctcggcat
    21301 cttcgtataa actaatgata ttaaagattt taagcgccga aatttgctcc cactccgccc
    21361 gatgactgcc gtacaaactg cgccgccaca atccttgcgg cgtaccgatc cagagacggt
    21421 tgcggctgtc ggccaacacg gtttgcacat tgtcggcagg cgtggacaag tgcgcgattt
    21481 gcaaatt
//
LOCUS       DAORLF010000024        20842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1372_length_20842_cov_2.155674, whole genome shotgun sequence.
ACCESSION   DAORLF010000024 DAORLF010000000
VERSION     DAORLF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20842
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1372_length_20842_cov_2.155674"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>303
                     /locus_tag="PK198_03725"
     CDS             23..>303
                     /locus_tag="PK198_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37875.1"
                     /translation="MEIITYYLLKTWGLNNQISIERGLAEYGNPDITHNVEYSLHPIV
                     RSSFVTVDKSEKSITSNIILKALSASGFDLTGFERKNNQLLSNNILRNA"
     assembly_gap    304..405
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <406..1104
                     /locus_tag="PK198_03730"
     CDS             <406..1104
                     /locus_tag="PK198_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37876.1"
                     /translation="QNKKPYAMFECKRVGVEEGMTKGPQTIEKAKQGAYVARTASSLQ
                     KIRSESGEMQGIIYKSDGSYIIKPYIHLMEEVIFSSDKELLRKFILTIGVVSNHGNWI
                     KKTADGDLSFSEENFQKELMVLAQSYDWLLFLTDKGLSDFIDKLLLHPIPEFQFLRDT
                     FLSSYTEGKKKNQFTKVQMNIEADRILLKYFSDNLATVESWFNVISPSQKSLTILKDE
                     LDELKNKNWQNILI"
     assembly_gap    1277..1295
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1296..1684
                     /locus_tag="PK198_03735"
     CDS             <1296..1684
                     /locus_tag="PK198_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HRF37877.1"
                     /translation="IYVNPPYGIDKERGTTIKNWLAKCAYANEEYNSEVLALVPIAAN
                     TAHWKKYVFTKAKSICFLYDTRLRFLENGQDTGKGAPMACAMIYWGNNQSRFYEVFIE
                     HGAVVDISNLIGERIGADRKKYELFH"
     gene            1809..2195
                     /gene="yiaA"
                     /locus_tag="PK198_03740"
     CDS             1809..2195
                     /gene="yiaA"
                     /locus_tag="PK198_03740"
                     /inference="COORDINATES: protein motif:HMM:NF008482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HRF37878.1"
                     /translation="MQQKTSQAFIAASWIALGAGMIGYLVGLYRAEMVLNEKGYYFTV
                     LMFGLFSVVSLQKAVRDRLENIKVTDLYYGICWFATILSIVLLVIGLWNATLLPSEKG
                     FYAFSFLLALFGAIAVQKNTRDNMDN"
     gene            2324..3226
                     /locus_tag="PK198_03745"
     CDS             2324..3226
                     /locus_tag="PK198_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRF37879.1"
                     /translation="MSKYLAVLLTAVFTIACQYSPEKSASGSVESLEESWADMSPESD
                     IEVEPPRTAEPPPPPPVPFPYTDEFGEGQPAGRTDLPPARKIIRNANVSISVTSYEKS
                     SAALRQLAQRLGADISNEAEERSSWRIANHFVIRIAPDKLDELLAELEKMALHVDSKS
                     VDSRDVTREYLDIETRLSVKRSTLERYREILRSAKTVTDILAVEEQIRVLIEDIESAE
                     TQLRGLRDEVQRSTVYLNMYQEFARPDVARPSFWNRIGNGFTEGWHTFLDIVVGLVYV
                     WPIALLLGLGVWWLLRKIRRRRAA"
     gene            complement(3377..4447)
                     /gene="serC"
                     /locus_tag="PK198_03750"
     CDS             complement(3377..4447)
                     /gene="serC"
                     /locus_tag="PK198_03750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRF37880.1"
                     /translation="MKIHNFSAGPAILPASVMEASARAAVDFNGMGLSILEISHRTKD
                     FEAVMAEAESLVRELSGAGDDYAVLFLGGGASTQFFMAPMNLLNDEDTACYVDSGVWA
                     TKAIKEAKNFGKIEVLASSKDRNYNYIPKDYAVAPQARYLHLTSNNTIYGTEIHEWPE
                     TDAPIVSDMSSDMFSRPIDIERFGLIYAGAQKNLGPAGVTLVIVRKDLLGHTTRKLPA
                     MLDYRIHADNGSMYNTPPVFPIYTLMLTLRWIKEQGGLEAMALHNVQKAQLLYNEIDT
                     NPCFTGTAVEEDRSLMNVCFVPVNPEHEAPFMEACKAAGISGVKGHRSVGGFRASIYN
                     AMPQESVQVLVDVMRDFAKKVG"
     gene            complement(4547..>4998)
                     /locus_tag="PK198_03755"
     CDS             complement(4547..>4998)
                     /locus_tag="PK198_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37881.1"
                     /translation="TAHLPGKASHETKFDTIHNAFYAEQAAKYRVEYYHQNGADHYLL
                     VSRPAPSLKVKRVGIGIHARMAGDSLAHYNEVFRTWKMEEPQLAQKGALLFNLMVQGE
                     DLSPYYTQNSGQEEYIEFPDAHTSFDTLRRQWISTLSDPLAPYRGQE"
     assembly_gap    4999..5049
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5635..7026)
                     /locus_tag="PK198_03760"
     CDS             complement(5635..7026)
                     /locus_tag="PK198_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37882.1"
                     /translation="MKKVLSLLAFAAVISFFACEKDEPVDPTPTLNELSGDLTSRTLT
                     SDKKWLLKGQVFVRSGQVLTIEPGTVIFGDKATRGTLVIDKGGKIEAVGTADKPIVFT
                     SNLAEGIRDRGDWGGLVILGRANCNQIDPGVEGITPAVTFGTNNNAANDGESSGTLKY
                     VRVEFAGIELTPNNETNSITMGGVGRGTVMEYCQVSFGGDDGYEWFGGTVNGKYLISF
                     CTWDDDFDVDYGWAGNVQYGLAVRYPGFADQSQSNGFETDNGPNDNDVTPYTDGVFSN
                     MTVVGPIKTGSSTSNANYGHAFDARRRTALSLFNSVLVGFPRGIRFNQPSVLAQYQSG
                     RGVLANNVLVAPATATAYNVGTGVTAADVQAYWTANNTTIDGPASDAIHQALGLNLDV
                     FFGARLADAYTAPDFRVTTGTLATGAAFSNAKFNEANRSAFFNKNVTFRGAFGSTDWT
                     TGWAEFKPVSKAY"
     gene            complement(7072..9888)
                     /locus_tag="PK198_03765"
     CDS             complement(7072..9888)
                     /locus_tag="PK198_03765"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF37883.1"
                     /translation="MKSFRLLFILLTLSTAAFSQANLFGTLSGVILDKSSKETIIGAN
                     IMVVGTEFGTATDFDGFFAINNVPAGKYTVQISYIGYENITISDVEVKAGENFKLDVV
                     MSSGSLVLEEITVTEYRKTNTVQAVLLEVKQAKQVVSGVSSQQITRSQDNNAAQVMAR
                     IPGVTIVDGRFVMIRGLSERYNNVMINNVTAPSTEVDRRTFSFDLISTSALDRMLIYK
                     SGSAEYPGDFAGGVIKLFTVEDVENNFTRLNVGLGYRANTSLKPYWQSKGSPTDFLGF
                     DNGFRQLPSNFPATRALKESGRNAQLRQGAAHSLPNNWQPTEGMATPDYSVGFTLGRN
                     MTLGGKKLSTITSINYSTSFQHFNRDFNRYFSWEDQTRPIDLRFQYNDASYEKQNRIS
                     ILSNWKWKSGNRSTITFKNLFNQIGENETILRSGFDFIQRPNDNLQNYLLGYRMRSIY
                     TGQLEGNHQLSDLSALRWVAGGSFLRELEPDLRRFRTFRPKDQGDQNFIMQLPPSSNL
                     FDTGRYYGDLMEFSANHGMDYSRKLPGVGSKGATLKGGYYADYRYRDFDSRYVSYLYP
                     GFFDPAVREQLERLPLDEVFSNENIRTNNGFVLEEGTRGIDSYNASSITGAAYLSGEA
                     SVGRIDLTGGLRGEYNVQTIKTVDDVVGLLKVNNPTFALLPFVNAGLNLNDDNLLRLA
                     YSRTVNRPEFRELAPFVFYDYKLEAGRFGNPDLQSAGIHNIDLRYEMYPRPGETFSVG
                     LFYKYFNNPIENKTVITTEQPQFTYINADFARSYGIEVELRKSLRGMTGSAFLDRFSV
                     NANASLIRSTVDLGASATAQDRVRPLPYIVNVALYYEEPVSNLNAALIYNIFGDRIFS
                     VGDVLFPTIYELSRNSLDFTITKRVKRVSYKLGIQNILDAPFRFFEDSDRTEKITLDR
                     DNPIIAFRRGQLASLSLTFDLNKP"
     gene            10230..10874
                     /locus_tag="PK198_03770"
     CDS             10230..10874
                     /locus_tag="PK198_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRF37884.1"
                     /translation="MSQADQTDWPNLKRYAAENQKLQAMANAGDRVVFMGNSITEFWK
                     VTDSSFFAGKPYINRGISGQTTSQMLVRFRPDVIDLQPAVVVILAGINDIAENTGPIS
                     LEHIFGNIVSMVQLARANNIKVVLSSVLPANRFPWRPQIKPADKVIQLNAMLEAYCLK
                     NKVVYLDYYSKMVDEHKGLDKKYGDDGVHPTLAGYRMMEPLLEAAVQKAVRKKR"
     gene            complement(10921..11490)
                     /locus_tag="PK198_03775"
     CDS             complement(10921..11490)
                     /locus_tag="PK198_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37885.1"
                     /translation="MKFEDIPAITLYAVCAVTGGIVLYHFITPAGDLSFVFLLPIFCI
                     VSLISWFLPVSFAPLGIKKPFRGSANWATLMLLVWCTEYGYNYAMNRSKVVLFADFYW
                     DAGMDLYLRENGTYKAVERGWIADDLKYGKYRIDGNRIILEGDLYIGIARMNDTLIYD
                     SKGLHFKLDSEWKGIHGDVMAIQINELFD"
     gene            complement(11591..12364)
                     /locus_tag="PK198_03780"
     CDS             complement(11591..12364)
                     /locus_tag="PK198_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF37886.1"
                     /translation="MNTQVKSMAEMAAAGESPEILFWVGCAGSYDARAQKVTLAFAEI
                     LQKAGISFAILGNEERCTGDPARRAGNEFVFQMLALQNIETLNMYEVKKIVTACPHCF
                     NTLKNEYPALGGHYEVIHHTQLIHQLIAEGRVKMKEGGAFKGKRITYHDSCYLGRVNG
                     VYEAPRAVLEALDADLVEMKRSKTNGLCCGAGGAQMFKEDEPGRERINVERAEEALAT
                     GASVIAVNCPFCLTMMSDGVKAKDKQDDVMVYDLSEMIV"
     gene            complement(12451..>13399)
                     /locus_tag="PK198_03785"
     CDS             complement(12451..>13399)
                     /locus_tag="PK198_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF37887.1"
                     /translation="TVAFLARRNVLKVERFQKPELKGWPFKDANIILWLEMALVFFIF
                     LMNSADMALHQNEYGFAVSRYLSAIWSGTGEGTLQLLERIGWWGHIAVVFAFLNYLPA
                     SKHLHILFAFPNTWFARQTPRGEMENMPEIMNEVKSMLGLAPETAASGELPEFGANDI
                     FGLSWKNLLDAYSCTECGRCTAACPANLTGKKLSPRKIVMDVRDRMEEVGRNIRSGDQ
                     QFAKDPEKPLGRDNYQDGKTLFDYISREEIHACTTCNACVEACPVLINPLEPILQLRR
                     YEILTESAGPGDWLPMFNSLESNGAVWAMPVERDAWVRG"
     assembly_gap    13400..13496
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13497..13770)
                     /locus_tag="PK198_03790"
     CDS             complement(<13497..13770)
                     /locus_tag="PK198_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HRF37888.1"
                     /translation="MLSTILFIIVSIVAFGYAARQFSRLIENIRLGKPENISGETGQR
                     WRNMLLVALGQQKMFKNWTPAILHLFIYVAFVITQIELIEIFADGIT"
     gene            13860..14354
                     /locus_tag="PK198_03795"
     CDS             13860..14354
                     /locus_tag="PK198_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRF37889.1"
                     /translation="MKILNQHQIEQKIRRLAIQILEQNYDEPEVILAGINNNGFAFAG
                     LLLQHLQQLSSIRFTLTRIRLNPAQPVAHPITTELPAEYFSGKVVIIVDDVANTGRTV
                     FYACQPLMQVLPKKVEVAVLVDRKHKFFPMYADYVGISLNTTLMEDIDVQIREVEEWA
                     VYLQ"
     gene            14674..16185
                     /locus_tag="PK198_03800"
     CDS             14674..16185
                     /locus_tag="PK198_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF37890.1"
                     /translation="MIRTLFFLFSLLAAGALSAQPITDSLAMRHARAVWEEFRELLAL
                     SNDAHFPDDIEKNVQWCETAFPRRGFSTRRLSTGGIPLLLAECKSPRKNAPTVLAYLQ
                     LDGQPVDPAHWQQESPWKATLKKQVEGQGWVEIPWQELHGQPDPDWRIFARSTSDAKG
                     PVMIFLAAMDALIASGRQLPYHLKVIMDFEEELGSPRMAQAVETHRNDLTADAFVIFD
                     GPPHISNERTLSFGARGIAEISISVFGPALPQHSGHYGNYCPNPALRLAQLLAGMKDE
                     QGRVSIPGFYDGIAIDENAKAEMARVPDNEAYIRAQLGIAQPDQVAGSYQEALQYPSL
                     NIRGMASGWIGDEVRTIIPSVATAELDVRLVMESDPERLIGLVRKYVEDQGYLVLDRK
                     PTQQERLKHDKICRFDSSVSYKAFRTELDSPTGQWLSRALVRAHGKAPIKIRTAGGSI
                     PISPFVTTLGVPAVAVPTVNPDNNQHSPNENIRVGNFIEGIKTILAVLTEPLR"
     gene            complement(16204..17271)
                     /locus_tag="PK198_03805"
     CDS             complement(16204..17271)
                     /locus_tag="PK198_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37891.1"
                     /translation="MPSKKPKTPQTPTATDTGNVYDRIFKEVMEDLALTILGRFHNLR
                     ITNLLRLPEKIHATTQREADFLCEVTDEHKNKFLLHIEFQSGHDRNMLARMQEYHGIL
                     ARKYKLPVRHFVFYFGDEPSKMPSKLEPEMVFTGFELIDFKTIDYRQLLDSDTGKEVI
                     LAILADFQNTPADAVIRLIISKLRQVSHSEQELKSSLNQLTILSRLRKLEAETIKITS
                     DMPITIDIKQDYLYNRGLEEGKLEGKLEGKLEGKLESEMITTIETDLELLCRMAKENV
                     SYDLMRRLSDVPELFLAAFCSTWSADKADILLVSVAQARRHSGIDNIRQHLMNTLEEF
                     GIRKDVSKPYIEGISNPEDIA"
     gene            complement(17369..>17634)
                     /locus_tag="PK198_03810"
     CDS             complement(17369..>17634)
                     /locus_tag="PK198_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37892.1"
                     /translation="TQDISRAVMLFPNPTTGRLQLSLEHEEQGRFTLELYNILGQRLY
                     RETVLKNGARLEHGLDIGHLAPGVYSLVLYTDTHRAVLKVVKE"
     assembly_gap    17635..17652
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17653..>17918)
                     /locus_tag="PK198_03815"
     CDS             complement(<17653..>17918)
                     /locus_tag="PK198_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37893.1"
                     /translation="SAGCSRTAVFNVPVTGPPVPVITRQGNILSSSSATAYQWYYNGN
                     PIPAAQGGTSQSIQPLVSGVFLVQVFAANGCSSVSNPFDFNLTT"
     assembly_gap    17919..18065
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18066..>18934)
                     /locus_tag="PK198_03820"
     CDS             complement(<18066..>18934)
                     /locus_tag="PK198_03820"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37894.1"
                     /translation="RRNNYITVLSAVSYTQLIEDAENINTAFQNWLVYNPDGDRAFEY
                     ADISSFGQGSRSVIFDNYSTDDDPSGTVDALVSPAINLSGMANPYLYFEHAYAQYSSE
                     YSDTLVLYYSTDCGATFTPFWYKGGRELATAPPTESSFVPNTNQWASNQIRLGFLAGQ
                     QRVHFLLVNISGWGNNLYLDEISFVDAQNFTNGAPQPNITTARRQVCAGETIFFEDIS
                     SNFPRQWAWQFPGGTPATSNVQHPFVTYNTPGTYSVGLGVSNSFGSNSGTANNYIQVL
                     PLPNITVSASQLP"
     assembly_gap    18935..19080
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19081..20315)
                     /locus_tag="PK198_03825"
     CDS             complement(<19081..20315)
                     /locus_tag="PK198_03825"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF37895.1"
                     /translation="MKKHIITLFFAALLLCIANMATAQSLGHFGQRLQARTSSYAPFL
                     NAKGQPSPTLSDHYEPAPAHRTCHAMEADADLRSRYPELGTLEEFEIQLQQRMAEYQN
                     QLQFRDPNNAVVTIPVIVHVVHNGEPVGTGANISQAQVMSQIAVLNEDYRRTGAGFNN
                     NPVGADMEIQFAPALRDPQGNLLAEPGIRRVNGNRSSWTYDAAQTVLKPNTFWDPNRY
                     CNIWTVQFGGDAQGLLGYAQFPSLSGLQGLEQNEGPASTDGVVIRWQAFGRTGNVQAP
                     FNGGRTTTHEIGHWLGLRHIWGDGNCSADDFCADTPNSAQPNYNCVAVTSCGSPDMIQ
                     NYMDYSNDACMNIFTQCQKLRMRTVLDNSPRRKELLSSTVHIGGGGSPMAPVAQFAAN
                     RTNICSGQQVQFTDQSSNNP"
     assembly_gap    20368..20462
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20559..>20842)
                     /locus_tag="PK198_03830"
     CDS             complement(20559..>20842)
                     /locus_tag="PK198_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF37896.1"
                     /translation="MEPILEMGEIKGGDLIPAGALALSTALAKAIPATALDREQAIRF
                     LRKEDPQLPDAPKGWLAAQYEGHTLGWLKGLGNRVNNYFPKEWRILGRF"
BASE COUNT         4749 a         5125 c         5513 g         4780 t
ORIGIN      
        1 aggcagagca ttgggtactc ttgtggaaat cattacctat tatcttttaa agacctgggg
       61 actgaataat caaatctcaa ttgagcgtgg acttgctgaa tatggcaatc ctgacataac
      121 ccacaatgtt gaatattcat tgcatcctat tgttcggagt tctttcgtaa cagttgataa
      181 atccgaaaaa tcaataacgt caaatataat tttaaaagcc ctcagcgcaa gcggttttga
      241 cttgacagga tttgagcgaa aaaacaatca attattaagc aacaatatac ttcgcaacgc
      301 ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaaaa caagaagcct
      421 tacgcaatgt ttgagtgtaa gcgggttggc gttgaagaag gaatgacaaa aggtcctcag
      481 acgattgaaa aagctaaaca aggtgcttat gttgcccgta cagcttcatc tttacagaaa
      541 atccgttctg aaagtggaga aatgcagggc atcatctata aaagcgacgg ttcttacatc
      601 attaagccat acatacatct gatggaagaa gtgatatttt cttctgataa ggagttgctg
      661 agaaaattca ttttgacaat tggagttgta agtaatcacg gaaattggat taaaaagacc
      721 gcagacgggg atttaagttt tagcgaagaa aactttcaaa aagaactcat ggtattggct
      781 caatcttatg attggttgct tttcttgact gacaaagggc tttccgattt cattgacaaa
      841 cttttacttc atccaattcc agaatttcaa tttttaaggg atacatttct ttcaagttat
      901 accgaaggca aaaagaaaaa tcagtttacc aaagttcaga tgaacattga agctgataga
      961 atattgctga aatattttag cgacaacctt gcaacggttg aaagttggtt caatgtaatc
     1021 tcaccaagtc agaaaagtct tacaatactg aaagacgaat tggatgaatt gaaaaataaa
     1081 aattggcaaa acatactaat atgagtgcag gaagaaacat aaacacatta agtcaaagct
     1141 ggggaacacc tcataaatac gttaatgccg tcaaagaagt atttggcgga cacattgact
     1201 tagacccatg ttcaaatgaa tattcagtag tagatgctca aacagagtat cgtttaccga
     1261 agcaagacgg attaaannnn nnnnnnnnnn nnnnnccata tacgttaatc caccttatgg
     1321 gattgacaaa gagcgtggga caacaataaa aaattggctt gctaaatgtg cttatgcaaa
     1381 cgaagaatac aactcggaag ttttggcact tgttccgatt gctgccaata cagcccattg
     1441 gaaaaagtat gtttttacca aagctaaatc tatctgtttt ttatatgaca caagattacg
     1501 ttttttagag aacggacaag acacaggcaa aggagcacca atggcttgtg ctatgattta
     1561 ttgggggaac aatcaaagta gattttacga agttttcatt gaacatggag cagttgtgga
     1621 catatcaaat ctaattggag aaagaattgg tgcagacaga aaaaagtatg aactgttcca
     1681 ctgacccgtc ggcacagagt tttacgctgc aaataaaatt accattactg gttcctgtag
     1741 catatctttg tactgatagc tgccgcgagg ggcggttgat gttcatcatt catcctaaaa
     1801 cttgagacat gcaacaaaaa acatcacagg catttattgc cgcatcctgg atagctttag
     1861 gcgccgggat gataggctat ttagtcggct tatacagggc agaaatggtt ttgaatgaga
     1921 aaggctatta tttcacagtt cttatgttcg gtttgttttc ggttgtttcg ctgcaaaaag
     1981 cagtgagaga ccggctggaa aatataaaag ttacagacct gtactatggt atatgctggt
     2041 ttgcaaccat attatccatc gttttattag tgatagggtt gtggaatgca acattattgc
     2101 ccagcgaaaa aggcttttat gcgttttcgt tcctcctggc attgtttgga gccatagccg
     2161 ttcagaaaaa caccagggac aatatggata attaagaagc aatgggatgt tgtgccgaca
     2221 ggttttagcg aaggagtgac cctgccagga tcacaacttg atctacgcgg aacacacgcc
     2281 aaaaagatca cttttctcat cttatttcca aaatcctctc accatgagca aatacctcgc
     2341 tgtcctgttg accgctgttt ttacaatagc ctgccagtac tcgccggaaa aatcggcttc
     2401 cggctcagtt gaaagccttg aagaaagctg ggcggatatg tctccggagt cagacataga
     2461 agttgaacct ccccgcactg ccgagccacc gccgccgccg ccggtaccat ttccatatac
     2521 cgacgaattt ggcgaaggcc agcctgccgg tcgcaccgat ctgccgccgg cgcgcaaaat
     2581 cattcgcaac gccaacgtct ccatctcggt gacgagctac gaaaaaagca gcgcagcgct
     2641 ccgccaactc gcccagcgcc tcggcgcaga catcagcaac gaagccgagg aacgctcctc
     2701 ctggcgcatc gcaaaccatt tcgttattcg cattgcgccc gataaattgg acgagctgct
     2761 ggccgaattg gaaaaaatgg ccctgcatgt ggatagcaaa agcgtggaca gccgcgatgt
     2821 gacccgcgaa tatttagaca tcgaaacgag gctctcggtg aagcgctcca ccctggaacg
     2881 ctaccgcgaa atcttgcgct cggccaaaac cgtgacggac atcctcgccg tggaggagca
     2941 aatccgggta ctcattgaag acatcgaatc ggcggaaact caactgcgcg ggctgcgcga
     3001 tgaagttcaa cgcagcactg tgtatctcaa tatgtatcag gagtttgccc gccccgatgt
     3061 ggcgcgccct tcgttctgga accgcatcgg caatggcttt acggaaggct ggcatacttt
     3121 tttagatatt gtcgtagggc tggtctatgt ttggcccatt gccttgttgc tggggctggg
     3181 ggtatggtgg ttgctgcgca aaattcgccg ccgccgggcc gcgtaagggt ataattctac
     3241 tgctcgctga ggacatgatg atcatataaa tccgcgtcta tccgcgtcga aaaaaagtat
     3301 aagtttccca gcatagtgaa ggggtgacag agctacaatg ctaagtcacc ccttcactaa
     3361 aacacacaat agaaactcag cccaccttct tcgcaaaatc ccgcatcaca tctaccagta
     3421 cctgcacgct ctcctgcggc atggcattgt aaatggaggc ccggaatccg cctaccgagc
     3481 ggtgcccttt cacgccggaa atacccgcag ctttgcaggc ttccataaac ggcgcttcgt
     3541 gttcggggtt caccggcaca aaacacacat tcatcagcga gcggtcttcc tccacagcag
     3601 tgcccgtgaa gcaaggattg gtatcaattt cgttgtacag caactgcgct ttctgcacat
     3661 tgtgcaaggc catggcctcc aacccgccct gctctttgat ccagcgcagc gtcagcatca
     3721 gggtgtaaat ggggaacacc ggcggcgtgt tgtacataga gccgttgtcg gcatggatgc
     3781 gataatcgag catggcgggc agtttacgcg tggtgtgtcc cagcaggtct ttgcgaacaa
     3841 tgaccaaagt gacgcccgcc ggaccgaggt ttttctgtgc gccggcataa atcaaaccaa
     3901 agcgctcgat gtctatcggg cggctgaaca tatcggacga catatcgctc acgatgggcg
     3961 cgtcggtttc gggccactcg tgtatttcgg tgccgtagat cgtgttgttg ctggtaaggt
     4021 gcaggtagcg cgcctgcgga gcaacggcgt aatctttggg aatgtagttg taattgcggt
     4081 ctttggaaga agccagaacc tcaattttac caaagttttt ggcttctttg atggccttcg
     4141 tggcccacac gccggagtcc acatagcagg ccgtgtcttc gtcgttgagc aggttcatgg
     4201 gcgccataaa aaactgagtg ctggcgccgc cgcccaaaaa cagcacggcg taatcgtcgc
     4261 ctgcgccgct gagttcacgc accaaacttt cggcttcggc cataacggct tcaaagtcct
     4321 tggtacggtg cgatatttcg agaatggaca atcccatgcc gttgaagtcc acggcggcgc
     4381 gggcagatgc ttccatcaca gaggcgggca aaatggcagg cccggcgctg aaattgtgta
     4441 ttttcataag tcaagtgatt ttgtgggagc cgctacgccc ccgaaaagca gggcaaaggt
     4501 agcgcttccg gcaggaaacg aaagccggaa acaggaaatt ttcgccttac tcctgccccc
     4561 ggtaaggcgc caatgggtcg gacagcgtag aaatccattg cctccgcagc gtatcaaaag
     4621 aagtatgcgc atcgggaaat tcgatgtact cctcctggcc ggagttttgg gtgtagtacg
     4681 gactgaggtc ctcgccctgc accatcagat tgaacagcag cgcgcctttt tgggccagtt
     4741 ggggttcttc cattttccag gtgcggaaca cctcgttgta gtgcgccagt gagtcgccgg
     4801 ccatgcgggc atgtatgccg atgcccacgc gcttcacttt caggctgggc gccgggcggg
     4861 aaacgagcag gtagtgatcg gcgccgtttt ggtggtaata ttcaacgcgg tatttggcag
     4921 cctgctctgc ataaaacgca ttgtgtatcg tatcaaattt ggtttcgtga ctcgctttgc
     4981 cgggcagatg cgccgtgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnng cacggcggtt gtcttggcat tgttcccaca ggaagagagg agaaataagg
     5101 cgcttgcgaa aagcagaaaa aaggtatttt tcatagaaaa aagtatttga ttggtgatcg
     5161 gtaggcggta gccggtgcca agcgtagagt gttcagtggc ggtaaacgcg agttttttta
     5221 accacgcctc cgtcgtggcg cagcgcgaat tttagcgaag gtcgcgaaat tccgcgaacc
     5281 acgcctgtcg tcgcggcgcg gtgcgagggg aggcgcgaaa cacacgcttt atttttgacg
     5341 ctgatcttta tgatttccgc tgctcggaga ggggacaaaa caatcataag aatctgcgtc
     5401 tttcagcgtc ggaaatctgg accttgtttt tggggggtct ccagcttagt gaaggggtga
     5461 ctacgggctg aaactcgctg agtcacccct tcactacccc cgaaacccgg aacccgaaac
     5521 ccgccttcgc caaggcttcg gcggatgaaa cccgaaacct tgaaccccaa aaacaaaaga
     5581 cttgccgccc acaacagcga gcgacaagcc tgtgctaaat aatgaatatg aaaatcaata
     5641 tgctttgctc accggcttga attcagccca gccggtcgtc cagtcggtgc tgccgaaagc
     5701 gccgcggaac gtgacatttt tgttgaagaa agcggaacgg ttggcttcgt tgaacttggc
     5761 gttgctgaaa gccgcgccgg tagccagcgt gccggtcgta acgcggaaat caggagccgt
     5821 gtaggcatcc gccaaacgag caccgaagaa cacatcgagg ttgaggccca atgcctggtg
     5881 aatggcatcg gaagccgggc cgtcaatggt ggtattgttg gccgtccagt aagcctgtac
     5941 atcagctgct gtaacgcctg ttcccacatt gtaggccgta gccgtagccg gagccacgag
     6001 tacgttgttg gccaatacgc cgcgtcccga ctggtactgc gccagtacgg agggctggtt
     6061 gaagcggata ccgcgcggga aaccaaccaa tacggagttg aacaacgaca aagcagttct
     6121 gcggcgagcg tcgaaagcgt ggccgtagtt ggcgttgctg gtagagctgc ccgttttgat
     6181 ggggcctact acggtcatgt tggagaatac gccgtcggtg tagggcgtca catcgttgtc
     6241 gttcgggccg ttatctgttt caaaaccatt ggattgcgac tggtcggcga aaccagggta
     6301 acgcactgcc agaccgtact gaacgttgcc tgcccagcca tagtccacgt caaagtcgtc
     6361 gtcccaggtg cagaaggaga tgaggtattt gccgttgacg gtgccgccaa accactcgta
     6421 gccgtcgtcg ccgccgaaag aaacctggca gtattccatc accgtgccgc gacctacgcc
     6481 gcccatcgtg atggagttgg tttcgttgtt gggcgtcagt tcgatgcctg caaactctac
     6541 gcgcacatac ttcagcgtgc cggagctttc gccgtcgttg gcagcgttgt tgttggtacc
     6601 gaaagtgaca gccggcgtga tgccttctac gcccggatca atctgattgc agttggcgcg
     6661 gcccagaata accaagccgc cccagtcgcc gcggtcgcgg atgccttcag cgaggttgga
     6721 agtgaacacg atgggcttgt cggcagtacc cacagcttcg attttgccgc ctttgtcaat
     6781 cacaagcgta ccgcgggtag ccttgtcgcc gaagataacg gtacccggct cgatggtgag
     6841 cacctggccg ctgcgcacaa aaacctggcc tttgagcagc cattttttgt cggaagtgag
     6901 cgtgcgggaa gtgaggtcgc cgctcagttc gttcagcgtc ggagtaggat caacgggttc
     6961 atccttttcg caagcgaaga aagaaatgac ggctgcgaaa gccaaaagac tcaatacctt
     7021 tttcataaac aattgtggtt aattagttga ttgtgagagt gcaaggagaa attacggttt
     7081 attcagatca aaagtgaggg agaggctggc caactgccct ctgcggaacg cgatgatcgg
     7141 attgtcgcgg tcgagcgtga tcttttcagt gcggtcggaa tcttcaaaga agcggaaggg
     7201 ggcatccaaa atgttctgaa tgccgagttt atagctcacg cgcttcaccc gcttggtgat
     7261 ggtgaagtcc aagctgttgc gcgaaagttc atagatggtg gggaagagca catcgcctac
     7321 ggagaagatg cggtcgccga agatgttgta aatcagtgcg gcgttgaggt tgctcacagg
     7381 ttcttcgtaa tacaaagcca cattgacgat gtaaggcaaa ggccgcacgc ggtcttgcgc
     7441 cgtagcagat gcgccgaggt ccaccgtaga gcggatgagc gaggcgttgg cgttcaccga
     7501 aaagcggtcc aagaaagcac tgcccgtcat gccgcgcagc gacttgcgca attccacttc
     7561 aatgccgtag ctgcgggcga agtcggcgtt gatgtaggta aattgaggct gctcggtggt
     7621 gatgaccgtt ttgttttcga tggggttgtt gaagtactta taaaacaagc ccacactgaa
     7681 ggtctcaccc ggacgcgggt acatttcgta gcgcaggtct atgttgtgaa tgccggcaga
     7741 ctggagatcg ggattgccga aacggccggc ttcgagtttg taatcataaa aaacgaaggg
     7801 cgccagttcg cggaattccg ggcggttcac cgtgcggctg taggccaggc gcagcaagtt
     7861 gtcgtcgttg aggttgaggc cggcattgac gaagggcagg agagcgaatg taggattgtt
     7921 cactttcaaa agtcctacca catcatccac tgttttgatg gtttgcacat tgtattcgcc
     7981 gcgcaggccg ccggtcaggt ctatgcggcc taccgatgcc tcgccgctca ggtaggctgc
     8041 gccggtgatg ctggaagcgt tgtaagagtc aatgccgcgg gtgccttctt ccaatacgaa
     8101 accgttgttg gtgcgtatgt tttcattgct gaacacttca tccaaaggca ggcgctccaa
     8161 ctgctctctc acagccgggt cgaagaagcc gggatagagg taagacacat agcgggagtc
     8221 gaaatcgcgg tagcggtagt cggcatagta gccgcctttg agggtggcgc ctttgctgcc
     8281 tacgccgggc aatttgcggc tgtagtccat gccgtggttg gccgaaaact ccatcaaatc
     8341 accatagtag cggcccgtgt cgaagaggtt ggacgagggc ggcaactgca tgatgaagtt
     8401 ttggtcgccc tgatctttgg ggcggaaggt gcggaagcgg cgcaggtcgg gttcgagttc
     8461 gcggaggaaa gagccgccgg ccacccagcg cagggcggag aggtcgctga gttggtgatt
     8521 gccctccaac tggccggtgt aaatagagcg catccggtag ccgagcaggt agttttggag
     8581 gttgtcgttg gggcgctgta tgaaatcgaa gccgctgcgg agaatggttt cgttttcgcc
     8641 aatctggttg aagaggtttt tgaaggtaat ggtgctgcgg ttgccggatt tccatttcca
     8701 gttggagagg atgctgatgc ggttttgttt ttcataagaa gcatcgttgt actggaaacg
     8761 caggtcaatg ggacgggtct ggtcttccca ggaaaagtag cggttgaaat cgcggttgaa
     8821 atgctggaaa ctggtggagt agttgatgga ggtgatggtg cttagttttt ttccacccaa
     8881 agtcatattg cgccccagcg tgaaacctac cgaatagtca ggcgtggcca tgccttccgt
     8941 aggttgccag ttattgggca ggctgtgggc ggcgccctgg cggagttggg cgttgcggcc
     9001 cgattctttg agtgctctgg tggcagggaa gttggagggc aattggcgga agccgttgtc
     9061 gaaaccgagg aaatcggtag gcgaaccctt gctttgccag taaggcttca gcgatgtgtt
     9121 ggcgcggtag ccgaggccta cgttgaggcg tgtaaagttg ttttctacgt cttctaccgt
     9181 gaacagcttg atgacgccgc ctgcaaaatc gccggggtat tctgccgagc cggatttgta
     9241 gatcaacatg cggtccaagg cgctggtaga gatgaggtca aaggagaaag tgcgccggtc
     9301 cacctcggtg ctgggcgccg ttacattgtt gatcatcacg ttgttgtagc gctcgctgag
     9361 gccgcgaatc atcacaaaac ggccgtccac aatggtcacg cccggaatgc gggccatcac
     9421 ctgcgcggcg ttgttgtcct gcgaacgtgt aatctgctgg ctcgacacgc cgctcaccac
     9481 ctgcttggcc tgcttcactt ccagcagtac ggcctgcacg gtgttggtct tgcggtattc
     9541 cgttacggtg atttcttcca aaaccaagga gccggaagac atgaccacgt cgagtttgaa
     9601 gttctcgccg gctttcactt ccacatcgct gatggtgatg ttttcatagc cgatgtagga
     9661 aatctgcacg gtgtatttgc cggcaggcac gttgttgatg gcaaaaaagc cgtcgaagtc
     9721 ggtagcagtg ccgaactcgg tacccaccac catgatattg gcgccgatga tggtttcttt
     9781 gctgcttttg tcgaggatga cgccggaaag cgtaccgaac agattggcct gcgaaaaggc
     9841 cgccgtactg agcgtcaaca gaataaagag tagccgaaag gatttcatac acttgtgttt
     9901 tgtaattgat tttcaaattc ggctgcaaag gaactgcggg tctttaaact ggaaattaat
     9961 gttgtgttaa gtgtgtgtaa agagttgaaa cggggttgaa atggcagtgc cggaacgggc
    10021 tttttgttcc ctgcaaaatg gaaggctctt gccggttcgg aagataagaa tacctttgag
    10081 gggcgtttcc gcagagccgt gaggatggca aggcacaggt agcggtttgg gcggagacgg
    10141 ggattgtttt attcgcacat ctgattatat ttcaattgtt tatgaagata tttatcacct
    10201 tcgtttgtat gttgatctca ctaaaaagca tgagccaggc agaccagacc gattggccca
    10261 acctgaaaag gtatgccgcc gaaaatcaaa agttacaggc tatggccaat gcaggggatc
    10321 gggttgtttt tatgggaaat tccatcaccg aattctggaa agtgacggac agctcttttt
    10381 ttgccggtaa accctacatc aaccggggaa tcagcggaca aacaacttcc caaatgctgg
    10441 ttcgtttccg cccggatgtg attgatctcc aaccggccgt ggtagtcatt cttgcaggaa
    10501 tcaacgacat tgctgaaaac acagggccaa tttcgctgga gcacattttt ggcaatatcg
    10561 tctcgatggt tcagttggcc agagccaata acatcaaagt ggtgctcagc tccgttctgc
    10621 ctgccaaccg gtttccgtgg cggccacaga tcaagcccgc agacaaagtg atacaactca
    10681 atgccatgct ggaggcttat tgcctgaaaa acaaggtggt gtatctggat tattattcca
    10741 aaatggtgga tgagcacaag ggcttagaca aaaaatatgg cgacgacggc gtgcatccta
    10801 cgcttgccgg atatagaatg atggagcctt tgctggaggc ggctgttcaa aaagctgtgc
    10861 gcaaaaaacg gtgagatcgc gcattcaacg cccgctcccc cgctcgtatc ttcgctacat
    10921 tcaatcgaat agctcgttga tctgtatggc cattacatct ccatgtatgc ccttccactc
    10981 cgagtctaat ttgaaatgca aaccttttga atcatagatc agggtgtcgt tcatgcgcgc
    11041 gatgccgatg tataagtcgc cctccagaat aatgcgattt ccatctatgc gatacttgcc
    11101 atatttcaaa tcatctgcta tccatccacg ttcaactgct ttgtaggttc cgttttctct
    11161 caaatacaag tccatgccgg catcccagta gaaatctgca aacaaaacga cctttgacct
    11221 attcatggca taattgtaac cgtactcagt acaccacacc agcagcatca aagttgccca
    11281 gtttgcgctc ccccgaaatg gctttttgat tcctaaaggt gcaaaactga ccggtaaaaa
    11341 ccatgagatc agcgaaacga tgcagaagat gggaaggaga aagacaaaac taagatcgcc
    11401 cgccggtgta ataaaatggt aaagtacgat accgcctgta accgcacaaa ctgcatacag
    11461 cgtgatggct gggatgtctt caaatttcat agcgcttgtt gtttagctca ctgaaaaact
    11521 aaccggggcg gtagagttgt tagctttggc cggcaattac acagccaaag ccaacagttc
    11581 tcgcttggtc tcacacaatc atttcactca agtcgtacac catcacatcg tcctgtttgt
    11641 ctttggcttt cacgccgtcg ctcatcatgg tgaggcagaa agggcagttg acggcaatga
    11701 ccgaggcgcc cgtagccagc gcttcttcgg cgcgctccac gttgatgcgt tcgcggccgg
    11761 gttcgtcttc cttgaacatc tgcgcgccgc cggccccgca acagaggccg ttggttttgg
    11821 agcgcttcat ttccaccaaa tcggcatcca aggcttcgag tacggcgcgg ggcgcttcat
    11881 acacgccatt gacgcggcct aagtagcagg agtcgtggta ggtgatgcgc ttgcctttga
    11941 aagcgccgcc ttccttcatt ttcacgcgcc cttcggcaat gagctgatgg atgagttggg
    12001 tatggtgaat gacttcataa tggccgccga gggcagggta ctcgtttttg agggtgttga
    12061 agcagtgcgg gcaggccgtg acgatcttct tcacctcgta catgttcagc gtttcgatgt
    12121 tttgcagggc cagcatctga aacacaaact cattgccggc gcggcgggcg gggtcgccgg
    12181 tgcagcgctc ttcattgccc agaatggcaa acgatatgcc tgctttttgg aggatttcgg
    12241 cgaaagccaa agtcactttt tgggcgcggg catcgtaact gcctgcacat cccacccaga
    12301 aaagaatttc cggcgattca ccggcagcgg ccatttcggc catagatttt acttgtgtat
    12361 tcatggttgg ttttgtgtta aggttggttc cgggtttcct cgaaatcaat ccctctgttt
    12421 ttctaaaaca cacagctcgc agctcgttat tcaccctctc acccacgcat cccgctccac
    12481 cggcatggcc cacaccgcgc cattgctttc gaggctgttg aacatcggca gccagtcgcc
    12541 gggaccggcc gattcggtga ggatttcgta gcggcgcagt tgcaggatgg gttccaatgg
    12601 attgatgagc accgggcagg cttccacgca ggcgttgcag gtagtgcagg cgtgtatttc
    12661 ctcgcggctg atgtagtcga acagcgtttt gccgtcctgg tagttgtctc tgccgagcgg
    12721 tttttccggg tctttggcaa actgttggtc gccgctgcgg atgttgcggc ccacctcctc
    12781 catgcggtcg cgcacgtcca tcacgatttt gcgcggcgag agttttttgc cggtcagatt
    12841 ggccggacag gcggcggtgc agcggccgca ttccgtgcag gagtaggcat ccaggaggtt
    12901 tttccaactg aggccgaaga tgtcgttggc cccaaattcg ggcagttcgc cgcttgcagc
    12961 cgtttcgggc gccagaccca gcatggattt cacctcgttc atgatttcgg gcatgttctc
    13021 catttcgccg cggggcgttt gccgggcaaa ccaggtattg ggaaaggcaa acagaatgtg
    13081 caggtgcttc gacgcgggca ggtagttgag aaatgcaaac accacggcaa tatgtcccca
    13141 ccagccaatg cgctccagaa gctgcagcgt gccctcgccc gtgcccgacc agatagctga
    13201 gaggtaccgg ctgacggcaa acccgtattc gttttggtgc agggccatgt cggcgctgtt
    13261 catgaggaag atgaaaaaga cgagcgccat ttccaaccaa aggatgatgt tggcatcttt
    13321 gaaaggccaa cctttgagtt ccggtttttg gaaacgctct actttgagca cattccggcg
    13381 ggccagaaac gctactgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt
    13501 gatgccgtcg gcgaatatct cgatgagttc gatttgggtg atgacgaagg ccacataaat
    13561 gaacaggtgc aagatggcgg gcgtccagtt tttgaacatt ttctgctggc cgagggccac
    13621 gagcagcatg ttgcgccaac gttggccggt ttcgccggaa atgttttccg gtttgccgag
    13681 gcggatgttt tcgatgagcc gggaaaactg tcgagcggca tatccgaagg ccacgatgct
    13741 gacgattata aaaaggatgg tagagagcat aacaggcgaa ttttcgctaa agtagaaaca
    13801 atttcaaatt ggttgggtac tttctctcaa agttttattt tcaccacaaa ttaacggcaa
    13861 tgaaaattct caaccagcat cagatcgaac aaaaaatacg gcggctggcc attcagattc
    13921 tggagcagaa ctacgacgaa cccgaagtga ttctggcggg catcaacaac aacggctttg
    13981 cttttgccgg gctgctactc caacacctgc aacagctgtc ttctattcgc ttcaccctca
    14041 cccgcatacg cctcaatccg gctcagcccg tagcacatcc catcaccaca gaactgccgg
    14101 cggagtactt cagcggcaag gtggtcatca tcgtggacga tgtggccaat acggggcgca
    14161 cagtgtttta tgcctgccaa ccgctgatgc aggttttgcc taaaaaagtg gaggtggctg
    14221 tgctggtgga tcgcaaacat aagtttttcc cgatgtatgc cgattatgtg ggtatctcgc
    14281 tcaatacgac gctgatggag gatattgatg tgcaaatccg cgaggtggag gaatgggcgg
    14341 tgtatctcca atagtttatc tcacttcgtg gtaatcggct gcgcagttca tctactttgt
    14401 agtaatctgc ttcgcagttt atctgcaaaa cgagatggca acgcggaacc agtagccgcg
    14461 ccgtggcgcg gggtcggcgg tgccaaacgc gagggaaacc cggaaccttg cacccgaaac
    14521 gcgatttttt tttgacgctg atctttatga tttttatgat ttccgctgct cggaaagggg
    14581 cataacaatc ataagaatct gcgtctctca gcgtcagaaa tccgaaatca gcccgttccc
    14641 acaggcaaat cagaaatcag aaagtcgaat accatgatac gcaccctctt ctttctcttt
    14701 tcgctcctcg cagccggcgc attgtctgca caacccatca ccgactcatt ggccatgcgc
    14761 catgcccgcg ccgtttggga ggagttcagg gaactgctcg ccctgtccaa cgacgcgcac
    14821 tttcccgacg acattgaaaa aaacgtgcag tggtgcgaga cggccttccc acggcgcggc
    14881 ttcagcaccc gcaggctgag caccggcggc attccgctgt tgctggccga gtgcaaaagt
    14941 ccccgcaaaa acgcgcccac tgtgttggca tacttgcaat tggacgggca gcccgttgat
    15001 cccgcgcatt ggcagcagga aagcccctgg aaggcaacgc tcaaaaaaca ggtggagggc
    15061 cagggctggg tggaaattcc ctggcaggaa ctacatggcc aacccgaccc cgactggcgc
    15121 atcttcgccc gctcgacctc cgacgccaaa ggcccggtca tgatcttcct ggccgccatg
    15181 gatgcgctca tcgcctccgg caggcagttg ccttaccacc tcaaagtcat catggatttt
    15241 gaggaagaac tcggttcgcc ccgcatggcc caagctgtcg aaacccatcg aaacgacctg
    15301 actgccgatg ctttcgtcat tttcgacggg ccgccgcaca tcagcaacga acgcacgctc
    15361 agtttcggag cgcgcggcat tgccgagatt tccatctccg ttttcgggcc ggcactgcct
    15421 cagcacagcg gccattatgg caactactgc cccaatccgg cccttcgact ggcgcagttg
    15481 ctggccggca tgaaagacga gcaggggcgc gtcagcattc ctggttttta cgacggcatc
    15541 gccatagacg aaaatgccaa agccgaaatg gcccgcgtac ccgacaatga ggcctacatc
    15601 cgcgcacaat tgggcattgc acaacccgat caggtggcag gctcgtatca ggaggcgctg
    15661 caatatccct cgctcaacat tcgcggcatg gcatcgggct ggatcggcga cgaggtgcgc
    15721 accatcattc catcggtggc cactgccgag ttggatgtac gcttggtgat ggaaagcgac
    15781 ccggagcgcc tcattggttt ggtcagaaaa tatgtggaag atcagggcta cctggtacta
    15841 gacaggaagc cgacccaaca ggagcgcctg aaacacgaca agatttgccg cttcgattcg
    15901 tctgtttctt ataaggcttt tcgcactgaa ttggattcgc caacaggcca atggctcagc
    15961 cgggccctcg ttcgtgcgca tggcaaagcc cccatcaaaa tacgcacggc aggcggcagc
    16021 attcccattt cacctttcgt cactacgctg ggcgtaccgg cagtggcggt gccgaccgtc
    16081 aatcccgaca acaaccagca cagccccaat gaaaacatcc gcgtgggcaa tttcatcgag
    16141 ggcatcaaaa cgatcttagc agtgcttact gagcctctga ggtaaacggc tgggtgcaga
    16201 aagtcaggcg atatcttcgg ggttgcttat gccttcaata taaggcttag atacatcctt
    16261 ccgaattcca aattcttcca aagtattcat aagatgctgc ctgatgttgt ctatgcctga
    16321 atgtcggcgt gcttgtgcga ccgataccag aagtatatcc gctttatcgg cagaccaagt
    16381 ggagcaaaaa gctgccaaaa acaactccgg cacgtcactg aggcgtctca tgaggtcata
    16441 agagacattt tcctttgcca tacggcaaag caattccaga tcagtctcta tagttgtaat
    16501 catctcgctt tcgagtttac cctcgagttt accctcaagt ttaccttcaa gtttaccttc
    16561 ttccaaccct ctgttgtaaa ggtagtcctg tttgatgtct atggtgatcg gcatatcgga
    16621 tgtgatcttt atggtttcgg cctccaattt acggagtctg gacaagattg tcaactgatt
    16681 gagactactt tttaattctt gctcgctgtg gctcacttgc cggagcttac tgataataag
    16741 ccgtataaca gcatcagcgg gtgtgttttg gaaatcggcc agaatagcca gaatcacttc
    16801 tttacctgta tccgagtcca acaactgtcg gtagtcaatg gttttgaaat ctatcaattc
    16861 aaagccggtg aaaaccattt ccggctccaa cttcgaaggc attttgcttg gctcatcccc
    16921 aaagtaaaac acaaaatgac gaacgggcag cttgtacttg cgagctaaaa tgccatgata
    16981 ttcctgcatt cgggcaagca tattccggtc gtggccgctc tgaaattcta tgtgtagcag
    17041 aaatttattt ttgtgttcat cggtcacttc gcacagaaaa tcagcctcgc gttgggtggt
    17101 ggcatgtatt ttttcgggca accgaagcaa gttagtgata cgaagattat gaaaccgccc
    17161 aagaatggtc aatgctaagt cttccatcac ttctttgaaa atgcgatcat agacgtttcc
    17221 tgtatcagtt gcagtgggcg tttggggcgt tttaggcttt ttggaaggca taagtcgttg
    17281 atttagttgc gcaaaactac aaaacatttt catcgcacaa aataggcgga tatgtttccc
    17341 tgacagaaaa catatccgcc ccccgcactc actccttcac caccttcaac accgcccgat
    17401 gcgtatctgt ataaagcacc agcgaataca cgcccggcgc caaatggccg atgtctaagc
    17461 catgctccag acgcgccccg tttttcagca ccgtctcccg gtagagcctc tgccccagta
    17521 tgttgtacag ttccagcgtg aaacgcccct gctcctcgtg ttccagcgaa agctgcaaac
    17581 gcccggtggt aggattggga aacaacatga cggcacggct gatgtcctgc gtttnnnnnn
    17641 nnnnnnnnnn nnagtggtca gattaaaatc aaaaggattg gaaacgctgg agcaaccgtt
    17701 ggcggcaaat acctgcacca aaaacacccc cgacaccaat ggctgaatgg actgcgaagt
    17761 gccgccctgc gctgccggta tggggttgcc gttgtaatac cattggtaag ccgtggcgct
    17821 gctgctgctc agaatgttgc cctgccgcgt gatgaccggc acaggcggcc ccgtcacagg
    17881 tacgttgaag actgccgtgc ggctgcaacc ggcgctgtnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnncggca actggctggc cgacaccgtg atgttgggca ggggcagcac ctgtatgtag
    18121 ttgttggctg tgccgctgtt gctgccaaag ctgttggaca cgcccaagcc caccgagtaa
    18181 gtgcccggcg tattgtacgt cacaaagggg tgctgcacgt tggaggtggc aggcgtgccg
    18241 ccaggaaact gccaggccca ttggcgcggg aagttgctgg aaatgtcttc aaaaaagatc
    18301 gtctcgccgg cgcatacctg cctgcgcgcc gtggtgatgt tgggttgcgg cgctccgttg
    18361 gtgaagtttt gcgcgtccac aaaggaaatt tcatccaaat aaaggttgtt gccccagccc
    18421 gaaatattca ccaacaggaa atgcacccgc tgctgcccgg ccagaaaacc cagcctgatc
    18481 tggttggacg cccattggtt ggtattcgga acgaaggagg attccgtagg cggcgcagta
    18541 gccaattccc gcccgccttt gtaccaaaac ggcgtaaagg tggccccgca atcggtagaa
    18601 tagtacagca ccagcgtgtc ggaatactcg ctgctgtatt gcgcgtaagc gtgctcgaaa
    18661 tagaggtaag gattggccat gccgctgagg ttgatggccg gactcaccag cgcgtccacc
    18721 gtgccggaag ggtcgtcgtc ggtgctgtaa ttgtcaaaaa tcacactccg cgatccctgc
    18781 ccaaaagaac tgatgtctgc atattcaaag gctcggtcgc cgtcggggtt gtaaaccaac
    18841 cagttctgga aggccgtatt gatgttttcg gcatcctcaa taagctgtgt gtaagacact
    18901 gccgacagca ccgtgatgta gttgttgcgg cgggnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 gggttgtttg acgactgatc ggtgaactgc acctgctgcc cggaacaaat attggtgcgg
    19141 ttggccgcaa actgtgccac cggcgccatg gggctgccgc cgccgccgat gtgtacggtg
    19201 gacgagagca attccttgcg ccgcgggctg ttgtccaaca ccgttctcat acgcaacttc
    19261 tggcattgcg tgaagatgtt catgcaggcg tcgttgctgt agtccatgta gttttgaatc
    19321 atatcgggac tgccgcaact ggtcacggcc acgcagttgt agttgggttg ggccgagttg
    19381 ggcgtgtcgg cgcagaagtc gtcggcgctg caattgccgt cgccccagat gtggcgcaag
    19441 cccaaccagt ggccgatctc gtgagtggtg gtgcggcctc cgttgaaagg cgcctgcaca
    19501 ttgcccgttc gcccaaaggc ctgccagcgt atgaccaccc cgtcggtaga ggccgggcct
    19561 tcattttgtt ccaaaccctg caaacccgaa aggctcggaa actgcgcata gcccaacaag
    19621 ccctgtgcat cgccgccaaa ctgcaccgtc cagatgttgc aatagcggtt gggatcccag
    19681 aaagtattgg gcttcagcac cgtttgggcg gcgtcgtaag tccatgagga gcggttgccg
    19741 ttcacgcggc ggatgcccgg ctcggccagg aggttgccct gcggatcgcg gagggccggc
    19801 gcaaactgta tttccatgtc ggcgcccacc gggttgttgt tgaaacccgc tcccgtgcgg
    19861 cggtagtctt cgttcaacac agcaatctgc gacatcacct gcgcctgact gatgttcgcg
    19921 cccgtgccca ccggttcgcc gttgtgtacc acatgcacaa tgaccggaat ggtgacgacg
    19981 gcgttgttgg ggtctcggaa ttgcaactga ttctggtact cggccatgcg ctgctgaagc
    20041 tggatttcaa actcctccag cgtgcccaat tccgggtagc ggctgcgcaa atctgcatcg
    20101 gcttccatag cgtggcaggt gcgatgcgcc ggagcaggtt cgtagtgatc cgacagggtg
    20161 ggggagggct gacccttggc gttcaaaaat ggagcgtaag aagaagttct ggcttgcagg
    20221 cgttggccga aatggcccag agattgagcc gttgccatgt tggcaatgca cagcaacagg
    20281 gcagcaaaaa acagggtaat aatgtgtttt ttcatcggtt gtcaggtttg gtgaagttgc
    20341 aaaaatagtc aattattttt tatggcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nncgctctat accggcctgc tccatcatct ggaatttgaa tgctcgctat ggagcaggcc
    20521 ggtatccgct tccgtccctg ccgcaacgta gagcgcgttc aaaatcgccc caaaatcctc
    20581 cactccttcg ggaaatagtt gttcacccga ttccccagtc ccttcagcca gcccagcgtg
    20641 tggccctcgt attgcgccgc cagccatcct ttgggcgcgt cggggagttg cgggtcttcc
    20701 ttgcgcaaaa agcgaatggc ctgctcccgg tccagcgccg tcgcaggtat agctttcgcc
    20761 agcgccgtgc tgagcgccag cgcccccgcc ggaatcaaat ccccgccttt gatttcgccc
    20821 atttccaata tgggctccat gc
//
LOCUS       DAORLF010000025        20755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1382_length_20755_cov_1.776570, whole genome shotgun sequence.
ACCESSION   DAORLF010000025 DAORLF010000000
VERSION     DAORLF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_20755_cov_1.776570"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK198_03835"
     CDS             complement(<1..247)
                     /locus_tag="PK198_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRF37897.1"
                     /translation="MLNRIIILAAVVGMALGCNPNEDYTLGDLTPPTNIAINAEIVGQ
                     TAATPNGDGSGDVKFTITADGALNYRIDYDAADGISLD"
     gene            complement(260..1348)
                     /locus_tag="PK198_03840"
     CDS             complement(260..1348)
                     /locus_tag="PK198_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37898.1"
                     /translation="MKISKIQAIWVLLLTLSFGCTEFDGIDLDTTFVDSATSANLSKI
                     FQISNDNSGNVTITPLGDGFTSAVVNFGHGTGAAASATLSPGRNVTHSYPEGSYTVKI
                     DYVDIAGKVTSQTYQLQVTYRAPENLTVTLSGETKVKATALYAKSFLVFYGDVANETG
                     TPMAIGEEMPAHTYPATGGPFTLRVVALSGGAATATYTKTLFSVPIDFEHPQVDYFFG
                     TFGDGQQFEKTANPNAAGLNTSATVGKFTRGNQGWSGTYSPLNIPINFAYGKKIKVLA
                     YNPNPANVGKSLNVELEWSIDGPANGVAVLKVPFTTSNAWEELVFDFGTISAIPAGTK
                     FTQLVLRFNDSQDGGGSVIYVDNFRLTN"
     gene            complement(1373..2872)
                     /locus_tag="PK198_03845"
     CDS             complement(1373..2872)
                     /locus_tag="PK198_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF37899.1"
                     /translation="MKLHNLLIAGLILFFTACTKDFIEVSPAGQFLTENYYADESQAF
                     SALVGVYDVLRKNSGGFENMISMMNAGSDDHFAGGGGATDGAGIQGFSNYTLNPTIMP
                     ASFWNDHYQGIFRANALMQKLPQTSMDAVRKARFEGEAKALRAIYYFNLVRLFKNVPL
                     ILEPLTAGNMYSVKQSTPAEVYAQIEKDLAEAIPVLPPTVNIATDAGRLTKGAAQAML
                     GKALLYQGKGAQAAAQLAEVNGQPGGTSQYGYRLLSNFGDLWVTDNKFNSEFILECTH
                     TSAGNSDWGWWGSSADEGNSLNVMVGPRSYSRPAGSNAPDLPSGWSFNVFTQDFYDAI
                     KTDPRFEATVFDLKTLSEAGEAAYIAGYQNTGYFLNKFLPRRSDVRTGGGALELNYRQ
                     NTPIIRLADTYLLEAEALGGTGTRAQALLDAVRARVGLPSVPVSLAAIKAERRLELAG
                     EGHRFFDLVRWGDAAAKLGSRGFTANKNEVFPIPERELLGTQLVQNQGY"
     gene            complement(2897..>3370)
                     /locus_tag="PK198_03850"
     CDS             complement(2897..>3370)
                     /locus_tag="PK198_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HRF37900.1"
                     /translation="YTFGLTVNLDYKGFDLMVFAQGAGGNKIFQGLRRLDIGNSNYQT
                     VALSRWTGEGSSNTFPRLTSNDSNGNFGRMSDFYLEDGTYARIKLVQLGYSLPKSIAD
                     KISAGRIRLYITGENLLTLTKYTGYDPEIGGGVFGIDKGFSPQARSFIGGIQVQF"
     assembly_gap    3371..3521
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3522..3853)
                     /locus_tag="PK198_03855"
     CDS             complement(<3522..3853)
                     /locus_tag="PK198_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37901.1"
                     /translation="MDLFDKTTTGILRPINIPGYVGVSSQPVGNVADMKNRGVELELG
                     YRKKVGDLNISANGSFSYLKNEVTYVASDADFIVGDASFQSMGVVTRTAVGQSYNSFY
                     GFQTNGIF"
     gene            complement(3840..>4206)
                     /locus_tag="PK198_03860"
     CDS             complement(3840..>4206)
                     /locus_tag="PK198_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF37902.1"
                     /translation="TGIIRRDGSSRFGANNKYGVFPSVSAGWVLSNEDFWQSGKTISH
                     LKIRGGYGVVGNDAIRDFGYLATVAGGFNYTLGNSGIITTGYAPTSLDNPDLRWEETS
                     QYWFRGRVVQQPHAQCGPV"
     assembly_gap    4207..4376
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4377..>5446)
                     /locus_tag="PK198_03865"
     CDS             complement(<4377..>5446)
                     /locus_tag="PK198_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF37903.1"
                     /translation="VVVDAGGIGYLNQSDIESIEVLKDAASAAIYGTRAATGVILVTT
                     KKGKEGKFNVSYNGFMGTSAPEKVLKLLNASQYAALLNERSVAGGGGLLFPSINLGTG
                     TDWQKEVFNYGAQRYSHELSLSGGNNRSTFYVSAGIQDQQGIVATEISNFNRTSFRLN
                     STHKISKIFTFGQTLGYSHQKATGIGATDEYGGVLSSAINLDPITPVVVTDPAVANTA
                     PYSVNPVIRDANGNPYGISSLVGQEMRNPTAFIQTRLGSYDWADDIVGNAYLQADITK
                     DISVKSSVGIKRAYWGGVGFTPVFYLSATSSTSQNSYGKSENKVFNWNVENTISYNKA
                     FNEHQISVLLGQGAYVENIGGG"
     assembly_gap    5447..5475
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5476..5992)
                     /locus_tag="PK198_03870"
     CDS             complement(<5476..5992)
                     /locus_tag="PK198_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF37904.1"
                     /translation="MTFSKTLLFGLLMLAGSWTFAQRNVGGTVTDAKSKEPLIGANVL
                     VKGTSIGTITEVDGSWTLSVPAGSDVLIFSYTGYGEKEVRLGADNVINVAMEEGTLLD
                     EVIVVGYGIQKKSVVTGVISSLKTKDLEKVPSARVEQSLQGRVSGVTIAQNSGQPGSP
                     STIRIRGITTFG"
     gene            6254..>7318
                     /locus_tag="PK198_03875"
     CDS             6254..>7318
                     /locus_tag="PK198_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37905.1"
                     /translation="MMPYIALLLGALTCLTAPFPVYGQASNLGNPPVVNYPKSQTLAG
                     TQTWAIEEDGFGYTWFANNQGVLQFDGYRWELLPIANKTIVRSLACDAAEHKVYVGGQ
                     GEFGYFSPDARGVFQYTSLSATLDEPHQDFGDVWHTVVTEQGVFFRADHQVFRYHQKR
                     VRPLFNEEKSFNFLSQWGSEIILQDDKSQFYTFDGQSFTPRSRNGYFSLGRISAVLNR
                     PGASTLIVTLHNGIFEETPTGFIPWETDQDEYIRSRVVYCAGMMADQRIILGTSYDGL
                     LIINQDRKIETHLNKKNGLQNNTVLGVAATRKGNVWLGLDNGIDLVKLTSAFREFYPD
                     GELQGAGYSAVIHRQHLYLGT"
     assembly_gap    7319..7337
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7338..>7752
                     /locus_tag="PK198_03880"
     CDS             <7338..>7752
                     /locus_tag="PK198_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37906.1"
                     /translation="PWKSYYSPTEKHHFHTVEGARGQVWKLQELNGELLMGHHEGGFV
                     IDGLKARKITAANGIWKFVPLPDGALLAGYYEGLISLRKDNSGWKQTAQLQGFSESAR
                     ILETDKSRHLWIAHPYRGIFRISAGELSELRVKKDP"
     assembly_gap    7753..7800
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7801..>8360
                     /locus_tag="PK198_03885"
     CDS             <7801..>8360
                     /locus_tag="PK198_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37907.1"
                     /translation="QIYKIEGEIVVSDAEQFYAVHPDHLSLRPLAWLNAHIPVAGDLK
                     FMTEDDYHNIWYATRNETGLLVPRQTFQKGYDKYVINALNNRLPEGFQSVFTIDPYNV
                     VFPTDKGFILFNPSSYLHDTIAPMLFLSEVLISGGKDSVILSGAMNNQKSLYDFDLSH
                     RQNNLLIRYSVRNAPEINAMLFSHRIG"
     assembly_gap    8361..8388
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8389..9178
                     /locus_tag="PK198_03890"
     CDS             <8389..9178
                     /locus_tag="PK198_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRF37908.1"
                     /translation="EWAEWSAAPEFNLSQLSPGKYQVAIKAKSPNGVESRPIVVTICI
                     RPPWYLHSFALFLYVMAVVLAVWLRIRRLKKRHHLEKKDIIAENLEKEKGYLLDAEIS
                     REEINLLKNEKLEAELNFKNQELISYTYHLVNKNELISEIKRALNKLEPKFEHDKELK
                     KEYRHILQLIEQNADADADWDNFMQNFDQVHSDFFKRLTQEFHHLSPNDYKMCIYLRM
                     NLTSKEIAALMNISIRSVETNRYRLRKKLGLQPDANLTQFLLRY"
     assembly_gap    9323..9365
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9366..>10155)
                     /locus_tag="PK198_03895"
     CDS             complement(<9366..>10155)
                     /locus_tag="PK198_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37909.1"
                     /translation="YRDLKPWEWMYDSDLFGVQDPVTGKIGYCCVLGNLGEHLALNVY
                     MGAEGLNSYYELLEFGEDDPMMAGLRQSCISVSFEDREILSDKDRKFIKDLGLKYRGS
                     GQWVMARDYAPSWLPWYINEEQAAFLVHALQQAVAVSIRAEENPELLQFDEEQVLVRV
                     PRQTPEGLIWEDQIMDMPDADVSIAIPPDAALMKKAKKLKKQQGAMFLCLTYAPGPTR
                     EDAQSRPFFPMIGLVMDKQSGMIVAHEIFSPDDIEAKLQHWLVWI"
     assembly_gap    10156..10198
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10802..11254
                     /locus_tag="PK198_03900"
     CDS             10802..11254
                     /locus_tag="PK198_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37910.1"
                     /translation="MSAISNSISKKSSGIENSMLSILFIAGLLGAFYFLRQPSELSSS
                     SSQSEYTALALTAETPTPSDSYLFIDGAMERGIPVRFTIPAYNEQVRYVLELGNGVRR
                     EMTEPQTDYVYRRSGTYKVKLMAEYQGSEKLLHAETLYIGENSEVAGY"
     assembly_gap    11426..11445
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11446..12260)
                     /locus_tag="PK198_03905"
     CDS             complement(<11446..12260)
                     /locus_tag="PK198_03905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF37911.1"
                     /translation="MNPKERYRAWAAATAELPLCFQPWYLDAVTEGGVWDVALLESNG
                     AVVGVWPYFLKHKFGLPYITMPHFIKWMGPWLSPDPARRLGDAYEIVEKLRLQLPKTF
                     KISIDCHPDFQNWLPLYWAGFRQTTRYTYTLDLSGDYEAGFNRNMRRNIKKAEAELAV
                     STEGAFDEFYRLNRLSFQRQGKEPTYSRTLLERHIHSLQQNQSVQIFFARDAQKRLHS
                     TAALTWDTGRAYYHLSGDDPELRNSGAGILLVKHAMQWAARELQVPVFDFEGS"
     gene            complement(12257..>12492)
                     /locus_tag="PK198_03910"
     CDS             complement(12257..>12492)
                     /locus_tag="PK198_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF37912.1"
                     /translation="KLDYADYYDMVFDKVSPGGYILADNVLWSGKVISENRDADTEAL
                     HRFNQKIQADTRVENIILPIRDGILMARKLPGA"
     assembly_gap    12493..12618
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12619..12902)
                     /locus_tag="PK198_03915"
     CDS             complement(<12619..12902)
                     /locus_tag="PK198_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF37913.1"
                     /translation="MKDRPGELIRYCEAHTTPVSEVLYRLERETHLRTLSPQMLSGHL
                     QGQLLRMISAMIRPRQALEIGTFTGYSAICIAVGMPADSVLHTIEANVELR"
     assembly_gap    12920..13144
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13145..>13452)
                     /locus_tag="PK198_03920"
     CDS             complement(<13145..>13452)
                     /locus_tag="PK198_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37914.1"
                     /translation="SLPAIIATAEPDTITPGATSQLDATFNPDYRYEGTPDPSLSRTD
                     VHNPMATPVQTTTYRVKVTDEQGCSATALVTVVVRAPECREPFIYVPNGFSPNGDGRN
                     "
     assembly_gap    13453..13552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13553..>13781)
                     /locus_tag="PK198_03925"
     CDS             complement(<13553..>13781)
                     /locus_tag="PK198_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37915.1"
                     /translation="RNDGVYTLELTVEAENGCVQTTAQSFPVILVNASPPGPVAICRG
                     DTATLRIGSGAGQNLTYTWSPVQDIVSGAGTS"
     assembly_gap    13782..13924
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13925..>14143)
                     /locus_tag="PK198_03930"
     CDS             complement(<13925..>14143)
                     /locus_tag="PK198_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37916.1"
                     /translation="FQNQSNNATEYLWRFNDPAQPDAFSTNINPTFTFSDTGTYQITL
                     IANPGTVCADTTERSIRLLPNSLFPGFDV"
     assembly_gap    14144..14393
                     /estimated_length=250
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14394..15084)
                     /locus_tag="PK198_03935"
     CDS             complement(<14394..15084)
                     /locus_tag="PK198_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37917.1"
                     /translation="MKSIRALFFVMLLLLVGSAELKATHIVGGEMRYKCLGNNEYEIT
                     LVIFRDCFYGNPLAWFDDPASIGVFNASNVLLRQILLAPMGNDTLQPVLSGECFVAPP
                     SVCVHTTFYRDTVELEPISGGYTLAYQRCCRNQTIVNIVNPLATGATYSVTISEAALQ
                     QCNSSPQFNQWPPIYICAGIPIEFDQSARDIDGDSIVYRLCEPLQGANQANPMPQPPN
                     PPPYLPVTWVSP"
     gene            complement(15235..16440)
                     /locus_tag="PK198_03940"
     CDS             complement(15235..16440)
                     /locus_tag="PK198_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRF37918.1"
                     /translation="MQLNFSGKKALVRVDFNVPLDKQYNITDDTRIREALPTIRYIVE
                     QGGAAILMSHFGRPLAKLKEDGTINVEKFTLRHVVGRLSELLGRPVHFAENCVGVVAE
                     SAAAALQPGEVLLLENTRFHAEEEKGNEAFAQQLAALADVYVNDAFGSAHRAHASTTI
                     VAKFFGKDAKSFGFLMEKELKNAAQVLHHAERPLTAILGGAKVSDKIQLLDRLADFVD
                     TLVIGGAMAYTFFKAQGGATGNSLVEEDKLDLAMNLVAKAKEKGVKLLLPMDSVVADA
                     FAETAAHRIEPSNAISDGWMGLDIGPEARAAFTAAIAESKTIFWNGPMGVFEMAPFAN
                     GTKTIAEAVAKATQTTGAFSLIGGGDSVAAVNQMGLGDQVSFVSTGGGAMLEFMEGKV
                     LPGVKAIEE"
     gene            complement(16515..17501)
                     /gene="gap"
                     /locus_tag="PK198_03945"
     CDS             complement(16515..17501)
                     /gene="gap"
                     /locus_tag="PK198_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRF37919.1"
                     /translation="MAKRIAINGFGRIGRLTFRNLIANPEVEVVAINDLTDNPTLAHL
                     LKYDTVQGKFNGTVSADENNIYVNGKAIRALAEKDPTKLPWGEMNIDVVLECTGRFTD
                     EDKAAMHLQAGAKKVLISAPAKGNVPTVVMGVNDDAIKDGIKMYSNASCTTNCLAPMV
                     KVLDDAFGVEMGLMTTVHAYTADQNIQDAPHRDLRRARAAAVSIVPTSTGAAKAVGEV
                     LPHLKGKLNGTAMRVPVPDGSVTDFTALLKKPATKDEINAAFKAAADSYLQNILEYCT
                     DPIVSADILGSPYSCIFDADLTMVNGNMAKVVGWYDNEAGYSARMADLVVKI"
     gene            17779..17853
                     /locus_tag="PK198_03950"
     tRNA            17779..17853
                     /locus_tag="PK198_03950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17812..17814,aa:Glu,seq:ttc)
     gene            complement(18107..18454)
                     /locus_tag="PK198_03955"
     CDS             complement(18107..18454)
                     /locus_tag="PK198_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37920.1"
                     /translation="MEGVISRLKGEISLVFKTLQKATHDPPIPGLADCRHVLLSHRSD
                     FGNSYRWMGGQEPIGGIRIQTVRLNNSLSATVAIQYTDYNPENEENTYWGRNILTMVK
                     VNGIWKIDDVHWR"
     gene            complement(18488..>19028)
                     /locus_tag="PK198_03960"
     CDS             complement(18488..>19028)
                     /locus_tag="PK198_03960"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37921.1"
                     /translation="VSSDLASIKVKNPNKVVAWGSAYWQYFEDLDKIKTFEETPLTLK
                     KQLFREEASSTGPVLRTIENNRALRPGDKLIVRIELRVDRDMEYVHMKDMRASGFEPI
                     NVLSQYKWQGGLGYYESTGDAATHFFFDYLPKGTYVFEYPLRAAHNGDFSNGITSIQC
                     MYAPEFTSHSQGVRVTVGR"
     assembly_gap    19029..19062
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19063..>20462)
                     /locus_tag="PK198_03965"
     CDS             complement(<19063..>20462)
                     /locus_tag="PK198_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRF37922.1"
                     /translation="KLRIPQGQVMALTHRVTAQAGNYSDGEESSIPVLTNRMLVTETL
                     PMSVRGGQTKTFTLSGLRDAKSPTLQHHQLTLEFTSNPAWYAVQALPYLMEYPYECIE
                     QVFNRFYANTLAASVANSQPNIKKVFDSWKGSDALQSNLSKNQELKTALLQETPWVLA
                     AQKESEQRQNIALLFDLHRMAKEQKEALEKIAQRQLPSGGFAWFNGGQENWYITQYVV
                     EGLGHLRQLKALSAADNAQAMSVATKALKHIDEQLARQYEELEKLAKAGKTKLSDDHL
                     THLAIHYLYARSFFPEITLEKRAERARDYYAGQAAQYWLGKGIYQQGLLAMALHRSGN
                     AAATRSIVASLRERALRSEELGMYWKYDRGYFWYQMPIETHALMIEVFAEAAKDEKAV
                     DELRLWLLKNKQTTNWKTTKATASAVYALLGHGDNWLSADKAVQVSFDKNTQRLYADR
                     ITAAQNDAQAGTGYFK"
     assembly_gap    20463..20524
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20525..>20755)
                     /locus_tag="PK198_03970"
     CDS             complement(<20525..>20755)
                     /locus_tag="PK198_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRF37923.1"
                     /translation="FLGLAHTTDLKTSIFTRDVLTQKDLMVLPNAPRFVREGDEIEFT
                     AKVSNLTAGTLAGTARLELFDALSMQPVDALLG"
BASE COUNT         4341 a         4972 c         5227 g         4724 t
ORIGIN      
        1 tgtctaaaga aataccgtcg gctgcatcgt aatcaattct gtaattgagc gcgccgtcgg
       61 cagtaatggt aaatttcaca tcgccggagc cgtcgccgtt gggcgttgcg gcagtttgtc
      121 cgacaatttc ggcattgatg gcaatgttgg ttggcggcgt gaggtcgccc agtgtgtagt
      181 cttcgttggg attgcagccc aaagccattc ctacaacagc cgcgagtatg atgatgcgat
      241 tgagcatggt caatgttttt taattggtta atctgaaatt gtcaacgtag ataacactac
      301 cgcctccgtc ctgcgagtcg ttgaaacgca gcaccaactg cgtgaacttg gtacctgccg
      361 ggatggcgct gatcgtgccg aaatcgaaca ccaattcttc ccaggcattg gaagtggtaa
      421 acggtacctt caacacagcg acgccgttgg ccgggccgtc tatagaccat tccaattcta
      481 cattgagcga tttgcctacg ttggccgggt tgggattgta cgccaagacc ttgatttttt
      541 taccgtaagc aaaattgatc ggaatgttca gcgggctgta agtgccgctc cagccttggt
      601 tgccacgtgt aaacttaccc accgtagcgc tggtattcag acccgcagca ttgggattgg
      661 ccgttttttc aaactgctgc ccgtcgccga aagtgccgaa gaagtaatcc acctgcggat
      721 gctcgaaatc aatgggtacg ctgaacaaag ttttggtgta agtggccgtg gctgcgccgc
      781 cgctgagggc taccacccgc aatgtgaacg ggccgcccgt agccggatag gtatgcgccg
      841 gcatttcctc accgatggcc atcggcgtgc cggtttcgtt ggctacgtcg ccataaaaca
      901 ccagaaacga cttggcatac agcgccgtag ctttcacttt ggtttcgccc gacagggtta
      961 ccgtcaggtt ttccggtgcg cggtaagtga cttgcagctg gtaggtctga gaggtgactt
     1021 tgcctgcaat gtccacataa tcaatcttta ccgtgtagct gccctccggg taggagtgcg
     1081 tcacgtttcg gcccggactg agcgttgcgg aagctgcggc gcctgtgccg tgaccaaagt
     1141 tgacgactgc tgaagtgaac ccgtcgccaa gcggcgtgat ggtcacgttg ccggagttgt
     1201 cgttgctgat ttggaagatt ttagacaaat tggcgctcgt ggcgctgtcc acaaaagtgg
     1261 tgtccaaatc tatgccgtca aattccgtac aaccgaaact gagggtcagc aacaataccc
     1321 atattgcttg aatcttgctt attttcatcg tagttaagat tttaagcggt gattaatacc
     1381 cttgattttg aactaattga gtgcccaaca gctctctttc cggtatcggg aatacttcgt
     1441 ttttattggc tgtaaagcct ctggacccca gcttggcagc agcgtcgccc cacctcacca
     1501 agtcgaagaa gcggtggcct tcgccggcca attccagcct gcgttcggct ttgatagcgg
     1561 ccaaggaaac gggtacggag ggaagcccca cgcgtgcgcg cactgcgtcc aacaaggcct
     1621 gtgcccgcgt tccggtacct cccaatgcct cggcctccag caggtaggtg tcggccaagc
     1681 gaatgatggg tgtgttttgc cggtagttca attccagagc gccgccaccg gttctgacgt
     1741 cagagcggcg tgggaggaat ttgttcagga agtagcccgt gttttggtag ccggcaatgt
     1801 aggctgcctc gcctgcttcg ctcaatgttt tgaggtcgaa aacggtggct tcaaagcgcg
     1861 ggtcggtttt gatggcgtca taaaaatcct gtgtaaacac gttgaagctc cagccgctgg
     1921 gcaggtcggg cgcattggaa ccggcaggcc gggaatagct gcgggggcct accatcacgt
     1981 tgaggctgtt gccttcatcg gcagagctgc cccaccagcc ccagtcggaa ttgccggcgc
     2041 tggtgtgtgt gcattccaaa ataaattcgg aattgaactt gttgtcggtc acccacaggt
     2101 cgccaaaatt ggacaacagg cgataaccgt actggctggt tccgccgggt tgcccgttca
     2161 cttcggccaa ctgtgcggcc gcctgggcgc ctttaccctg atacaacaag gctttgccca
     2221 gcatggcctg cgcggcgccc ttggtgaggc ggcctgcatc agtggctatg ttgacggtgg
     2281 gcggcaaaac gggaatggcc tcggcgaggt ccttctcaat ttgcgcatac acctcagcgg
     2341 gcgtggactg cttcacgctg tacatgttgc cggcggtgag cggttccaga atcaaaggca
     2401 cgtttttgaa aaggcgtacc aaattgaaat aataaatggc tctcaatgcc ttggcctcgc
     2461 cttcaaaccg tgccttgcgg acggcgtcca tgctcgtttg cggcagtttt tgcatcagcg
     2521 cgttggctct gaagatgcct tggtagtggt cgttccagaa gctggcgggc atgatggtag
     2581 gattgagggt ataattggaa aacccctgaa tgcccgcgcc gtcggtagcg ccgccgccac
     2641 ctgcaaagtg gtcgtcggaa ccggcgttca tcatggaaat catgttctca aaaccgccgg
     2701 aatttttcct cagcacgtcg taaacgccta ccaatgcaga aaaagcctgg ctttcgtcgg
     2761 cgtagtagtt ttcggtgagg aactggccgg caggagaaac ctctataaag tctttggtgc
     2821 aggccgtgaa aaagaggatc agccctgcga tcaataaatt atgcaatttc atagagaaat
     2881 gaatttttag tgtggattaa aactgtacct gaatgccgcc gatgaaagac ctggcctgcg
     2941 gggagaagcc cttgtctata ccgaacacgc cgccgccgat ttcaggatcg tagccggtgt
     3001 atttggtcag ggtcagcagg ttttcgccgg taatatacag tcggatgcgg cctgcggaga
     3061 ttttatccgc aatacttttg ggcaaagagt agcccaactg aacgagcttg atgcgcgcat
     3121 aggtaccgtc ttcgaggtag aagtcggaca tgcggccgaa gttgccgttg ctgtcgttgc
     3181 tggtcagtcg cgggaaggta ttggagctgc cctcgcccgt ccagcggctc agtgctacgg
     3241 tttggtagtt ggagttgccg atgtctaagc gacgcaatcc ctggaagatt ttgttgccgc
     3301 ctgcaccctg tgcaaagacc atcaaatcaa agcctttgta atcgagattg accgtgaggc
     3361 cgaacgtgta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggaaaatg ccgttggtct
     3541 ggaagccgta gaacgagttg taagactgcc ccactgcggt acgggtaacg acgcccatag
     3601 actggaagga tgcgtcgcct acgatgaagt cggcatcaga agccacatag gtgacctcgt
     3661 ttttgaggta ggagaaactg ccgttggcgc tgatgttcag gtcgcccact tttttgcggt
     3721 agcccaattc taattccacg ccgcggtttt tcatgtcggc cacgttgccc acaggctgcg
     3781 aggatacgcc cacataaccc ggaatgttga tgggacgcag gatgccggta gtagttttgt
     3841 caaacaggtc cacattgagc gtgaggctgc tgaacaactc tgcctcgaaa ccaatattgg
     3901 ctggtctctt cccaacggag gtcggggttg tccaacgatg taggtgcgta accggtggtg
     3961 atgataccgg aattgcccag cgtatagttg aaaccgccgg ccaccgttgc cagataaccg
     4021 aagtcgcgga tggcatcgtt gcccactacg ccgtaaccgc ctctgatttt gaggtgagaa
     4081 atggttttgc cgctttgcca gaagtcttca ttgctgagca cccaacctgc cgaaacagac
     4141 gggaacacac catatttatt attggcgccg aacctcgatg agccgtctct tctgatgata
     4201 ccggtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagc
     4381 cgccgccgat gttttctaca taagcgccct ggcccagcaa tacagaaatc tggtgctcgt
     4441 tgaaggcttt gttgtacgaa atggtattct ccacgttcca gttgaacacc ttgttttcgc
     4501 tcttgccgta gctgttttgc gaggtgctgc tggtggcgct cagataaaaa acaggcgtga
     4561 aacctacgcc gccccagtag gcgcgcttga tgccgacgga agacttcacc gagatgtctt
     4621 tggtgatgtc ggcttgcagg taggcattgc ctacgatgtc gtcggcccag tcgtaagagc
     4681 ccaatctcgt ttgaatgaaa gcagttgggt tgcgcatttc ctggcccacc agcgaggaga
     4741 tgccgtaggg attgccgttg gcgtctctga tgacggggtt cacactgtat ggcgccgtgt
     4801 tggcaactgc cgggtcggtg accaccaccg gagtgatggg gtcgaggttg atggccgaac
     4861 tcaaaacgcc gccgtactca tcggtagcgc caatgccggt agccttttgg tgcgagtagc
     4921 ccagcgtttg cccgaaggtg aatattttgg aaattttgtg ggtggaattc aacctgaaac
     4981 tggtgcggtt gaagttggaa atttccgtgg ccacgatgcc ctgttgatcc tggatacctg
     5041 ccgacacata aaacgtagag cggttgttgc cgccgctgag gctcaactcg tgcgagtatc
     5101 tctgcgcgcc gtagttgaaa acctcttttt gccagtcggt gccggtcccc aaattgatac
     5161 tgggaaacaa cagcccgccg ccgccggcca ccgagcgctc attgagcagg gccgcgtact
     5221 gagaggcatt cagcaatttc aatacttttt caggcgcgga agtacccata aagccgttgt
     5281 agctcacatt gaacttgcct tctttgcctt ttttcgtagt cactaagata acgccggtag
     5341 ctgcgcgtgt gccgtagatg gccgccgaag cagcatcctt gagcacttcg atggattcga
     5401 tatcggattg attgaggtag ccgatgccgc ctgcgtccac caccacnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnncgcca aacgtagtaa tacctctgat gcgaatggta gagggcgagc
     5521 cgggctggcc ggagttctgc gcgatggtta ccccggagac gcggccttgc agcgattgct
     5581 caacgcgggc actgggcact ttttccaggt ctttggtttt caaactcgaa atcacgcctg
     5641 tcacgacgct ttttttctga atgccgtagc ctaccacgat cacttcgtca agcagggtac
     5701 cttcttccat tgctacgttg atgacgttat cggcgcctaa tctgacctct ttctccccat
     5761 atcctgtata ggaaaaaatc aaaacgtcgg aaccggccgg aaccgataat gtccacgaac
     5821 catccacctc ggtgatggtt ccgatgctgg tgcctttgac gagtacattg gccccgatca
     5881 aaggctcttt gctcttggcg tctgttaccg tgccaccgac attccgctga gcgaatgtcc
     5941 atgaaccggc caacatcaac agcccgaaca aaagtgtttt agaaaatgtc ataagcatgt
     6001 agatttaagt tgtaataatt tggtgagtgt agaactttgg caaatgtacc caagcgcccg
     6061 ccgatactcg ttttttccac tactacattg ctactacaag ctaagcaacg gcgtactaca
     6121 ataattcaac cataattttt taccccggca acatttctaa gagtatcaac agattgtgct
     6181 tgtttggtct tttttttcgc tgatatccgg gctttccata gttttgctgt acgaaagcag
     6241 cctgatgtat aaaatgatgc catacatagc actcctactg ggagcgttga cttgcctgac
     6301 agcgcccttc cctgtgtatg gccaagcctc caatctgggc aatccgcctg ttgtgaacta
     6361 tcccaaaagc cagactttag caggcacgca aacctgggcc attgaggaag acgggttcgg
     6421 atacacttgg tttgccaaca accagggcgt actccaattt gatggctacc gttgggaact
     6481 gctgcccatt gccaataaaa ccatcgtgcg gtctttggct tgcgacgccg ccgaacacaa
     6541 agtttatgtg ggcgggcaag gtgaattcgg gtacttcagc ccagatgcaa gaggcgtttt
     6601 tcaatacact tcgctctcag ctacgctgga tgagccacat caagactttg gagatgtgtg
     6661 gcacaccgtc gttacagaac agggggtgtt tttcagagcc gaccatcagg tgtttcgcta
     6721 ccaccaaaaa cgagtacgac cactttttaa tgaagaaaaa tcgttcaatt ttctgtctca
     6781 gtggggcagt gaaattatac ttcaggacga taaaagccaa ttctacacct ttgacggaca
     6841 gtcttttact ccccggtcgc gcaatggcta tttctccctc ggccgcatct cggccgtgct
     6901 gaatcgcccc ggcgcctcca cgctcatcgt caccctgcac aacggcattt ttgaggaaac
     6961 accgactggg ttcatcccct gggaaacaga tcaggacgag tacattcggt cgcgggtggt
     7021 gtattgtgcc ggcatgatgg ccgaccagcg catcatctta ggcacctcgt atgacggcct
     7081 gctgatcatc aatcaggaca ggaaaatcga aacccatctc aacaagaaaa acggtcttca
     7141 aaacaacacg gtgctgggtg ttgccgctac ccgcaaaggc aatgtctggc tgggtttaga
     7201 caatggaatt gatttggtta aactcacttc cgctttccgc gagttctacc ccgacggcga
     7261 gttgcagggc gcaggctaca gcgcagtcat tcaccggcag catctgtatc tcggcaccnn
     7321 nnnnnnnnnn nnnnnnnccc tggaaatcct actactcgcc caccgagaag caccatttcc
     7381 atacggtcga aggcgccagg gggcaggtct ggaaactcca ggaactgaac ggcgaactgc
     7441 tgatgggcca tcacgagggc ggttttgtca tagatggact caaagcacgc aaaatcacgg
     7501 cggccaacgg catctggaaa tttgtgcccc ttcccgacgg cgcccttttg gcgggctatt
     7561 acgaagggct tatttccttg cgaaaagaca attccggctg gaaacagacc gcccaattgc
     7621 agggcttcag cgagagcgcc cgcattttgg aaaccgacaa aagcaggcat ctctggatag
     7681 cccacccata ccggggcatt ttcaggattt ctgccggcga gttgtctgaa ctccgtgtga
     7741 aaaaagaccc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 cagatttata aaatagaggg tgaaattgtg gtcagcgatg ccgaacagtt ttatgccgtc
     7861 caccccgatc atctgagctt acgtccgctc gcctggttga atgcgcacat tcccgtagca
     7921 ggagacctga aatttatgac ggaagacgat tatcacaata tatggtatgc tacccgcaac
     7981 gaaaccggtc tattagtacc ccggcaaact tttcagaagg gctacgacaa atatgtgatc
     8041 aacgcgctca acaaccggct gccggagggc tttcaatcgg tattcaccat tgatccttac
     8101 aacgtcgttt ttcctaccga caaaggattt attcttttca atccatcctc ttatctgcac
     8161 gacactattg cccccatgct gtttctttcc gaagtactca tttccggtgg aaaagacagc
     8221 gtcatcctca gcggcgccat gaacaatcaa aaaagtctgt atgactttga tctgtctcat
     8281 cggcaaaata atctgctcat cagatattcg gtcagaaatg cccccgaaat caacgccatg
     8341 ttgttcagtc atcgcatagg nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aatgggcaga
     8401 atggtcggcg gctccggagt tcaatctcag ccagttatcg ccggggaagt atcaggtagc
     8461 catcaaagcg aaaagtccaa acggggtgga aagccggcca attgtagtga ccatctgcat
     8521 tcgtccgccc tggtatttgc attcgttcgc cttgtttttg tatgtcatgg cagtagtttt
     8581 ggccgtgtgg ttgcgcatac gcaggctgaa aaaacggcat catctggaaa aaaaggacat
     8641 catagctgaa aacttagaaa aagaaaaagg ctacctgctg gatgcagaaa tcagccggga
     8701 agaaatcaac ctgttgaaaa atgaaaaatt ggaagcggag ttgaatttca aaaatcagga
     8761 gttgatttca tatacctatc acttggtcaa taaaaacgag ttgatcagcg agatcaaacg
     8821 ggcgctcaat aagctggaac ctaagtttga acacgacaaa gagttgaaaa aagaataccg
     8881 gcacatcctc caactcattg agcaaaacgc agatgcagat gccgactggg acaattttat
     8941 gcaaaatttc gatcaggtac actccgattt tttcaaacgc ctcacccagg agtttcacca
     9001 cctcagcccc aatgactaca aaatgtgtat ctacctgaga atgaatctga cctccaaaga
     9061 aattgccgcg ctgatgaata tttccattcg cagcgtggaa accaaccgct accggctgag
     9121 gaagaaatta ggtttgcaac ctgatgcaaa tctgacgcag tttttgttga ggtattgacc
     9181 tctcattccc ctaccctacc ccataaaccg gaacatcatt tccagggcat cttcaaaaac
     9241 aggctcttca ggattgacca tcaggtctat gtcgagttcg tcggcgatgt cgtccagcat
     9301 atcggcagca ataacactgg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnaaatc caaaccaacc agtgttgcag cttggcctct atgtcgtcgg gagagaaaat
     9421 ttcgtgcgct acaatcatgc cgctttgctt gtccatgacc agaccgatca tgggaaagaa
     9481 ggggcggctt tgggcatctt cacgcgtggg accaggagca taagtaaggc acaagaacat
     9541 ggcgccctgc tgttttttca gctttttggc ctttttcatc aaggctgcgt cgggcggtat
     9601 ggcaatgctc acgtcggcgt cgggcatatc cataatctgg tcttcccaga tgaggccctc
     9661 cggcgtttgg cggggtacgc gcactaatac ttgctcttcg tcgaactgca gcagttcggg
     9721 gttttcttcg gcacggatag aaaccgccac cgcctgttgt agggcatgga cgagaaaggc
     9781 ggcctgctct tcgttgatgt accagggcag ccaactgggc gcataatcgc gggccatgac
     9841 ccactggccg ctgccgcgat atttcagccc caggtctttg atgaatttgc ggtctttatc
     9901 tgagagtatt tccctgtctt caaacgaaac ggaaatgcaa ctttggcgca agccggccat
     9961 catggggtcg tcttcgccga attccagaag ttcgtagtag gagttcagcc cctcggcgcc
    10021 catgtaaaca ttgagggcca aatgctcgcc caaattgccc agcacacagc agtagcctat
    10081 tttgccggtc acagggtcct gcacgccgaa gagatcggaa tcgtacatcc attcccaggg
    10141 cttgaggtcg cgatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
    10201 gcggttttct ttggtgatgt tcattgctga tgaagttaaa tgacgccgca agttaggaat
    10261 aaaatgccga ttaatccaag aaaatacggg cattacagcg tttgtaagat gtggcaaaac
    10321 ctcagcaaag gaggacaagc aatatccgtc ccggcatttt ttggcacgat tcttggtttt
    10381 aaaagaacat gaaacgcatt atgaaaaaag ataatgtttt tcataaaaac agagagggcc
    10441 gggcattgat atatgataat ttgttatatg tttgcgccgg gttcttattt ttttactaat
    10501 gaaacactat acaaacatga aaaacacttc taccaccgtc caccgctcgc cgtaaggtag
    10561 cttcaggcaa ggcgggtttt ccgattacat tttggtaata caactttcag cgcgaggaac
    10621 aaccattgtt cctcaaggca gcagcatgtc tgtcttttgc tgaggttctg aaaagctgca
    10681 cggcttttcc ggggattcgt ttgcctgtcc gcatcatgca ctttttatca tttccgttgg
    10741 ctgtgccctg cacggcttgg cggagctttt tccctcatca atttttcaaa tcaaaacggc
    10801 catgagcgca atttccaact ctatatccaa aaaaagcagc ggcatcgaaa acagcatgct
    10861 ttccattctt ttcatcgccg gcctgctcgg cgctttttac tttctccgcc aaccttccga
    10921 gctgtcctca agctcttctc agagcgaata cactgctctt gctcttaccg ctgaaacgcc
    10981 gaccccctcc gattcctacc tgttcattga cggcgctatg gagcggggca ttccggttcg
    11041 gtttaccatt cctgcctaca atgagcaggt gcggtatgta ctggagttgg gcaacggtgt
    11101 tcgccgcgaa atgacagaac cgcaaaccga ctatgtgtat cgccgttcgg gaacatacaa
    11161 agtgaaactc atggcagaat atcagggcag cgaaaaactg ctgcacgcgg agacgcttta
    11221 catcggcgaa aactcggagg tggcggggta ttgagtgctt tttgaggtaa cggctgctta
    11281 aactgggtat gtctgtcgaa gatattgccc acaagttggg gctttcttcg gaatacgttg
    11341 caggcctgag agcgcccatg cagaattaaa gccccttcca gaccctgtga taaggcatag
    11401 gctcggctcc gaattgccgg cgaatnnnnn nnnnnnnnnn nnnnnatact gccctcgaaa
    11461 tcgaataccg gaacctgtag ttcccgcgca gcccattgca tggcgtgttt cacgagcaag
    11521 atgcctgctc cgctgttgcg cagttcgggg tcgtcgccgc tgaggtggta ataggcgcgg
    11581 cctgtgtccc aggtgagagc ggcggtacta tgcaggcgtt tttgtgcatc acgggcaaag
    11641 aatatctgaa cggattgatt ttgttgtaag gaatggatat gccgctccaa caaagtacgg
    11701 gagtaggtcg gctccttgcc ctgccgctga aaactcaggc ggttgaggcg ataaaattcg
    11761 tcaaaggctc cctccgtgct gactgcaagc tcggcttcgg cttttttgat gttgcgccgc
    11821 atgttgcggt tgaagccggc ttcatagtcg cccgataagt ccagcgtgta ggtgtagcgc
    11881 gtagtttggc gaaagccggc ccaatacaac ggcagccagt tttggaaatc cgggtggcaa
    11941 tctatactga ttttgaacgt tttaggaagt tgaagcctca gtttttccac aatttcataa
    12001 gcatctccca agcggcgggc aggatcgggc gacaaccacg gccccatcca ttttataaaa
    12061 tgcggcatgg tgatgtaagg caacccgaac ttgtgtttga gaaaatacgg ccacacgccc
    12121 accactgcgc cattggattc cagtaaggcc acatcccata caccgccttc tgtcacggcg
    12181 tccagatacc agggctgaaa acaaagcggc aattcggcag tagcagcggc ccaagcccgg
    12241 tagcgttctt tcggattcat gcaccgggaa gtttgcgggc catcaaaatg ccgtcccgaa
    12301 tgggcagaat gatgttttcc accctcgtat cggcctgaat tttctgattg aaccggtgca
    12361 atgcttcggt atcggcatcg cggttttcag aaatgacctt gccgctccac aatacattgt
    12421 ctgccagtat gtaaccgccc ggcgacacct tatcgaacac catgtcgtaa taatcggcat
    12481 aatccaattt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnncg caattctaca ttggcttcta tggtgtgcaa cacgctgtcg
    12661 gcaggcattc cgacagcgat gcagatggcg ctgtagcctg taaaagtacc gatttccagc
    12721 gcctgccggg gccgtatcat ggcactgatc atgcgcaaca actggccctg caaatgcccc
    12781 gacaacatct gcggcgataa agtacgcagg tgggtttcgc gttccagccg gtacagcacc
    12841 tccgatacgg gcgtggtgtg tgcctcgcag tatctgatca actcgccggg tcggtctttc
    12901 acatttttcg ctttcatctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnngttgcg cccgtcgccg ttgggagaaa agccgttggg cacataaatg aaaggctcac
    13201 ggcattcggg agcgcgcacc accaccgtta ccaaagccgt agcagaacag ccctgctcat
    13261 cggtcacttt cacccggtaa gtagtggttt gtaccggcgt ggccatcgga ttgtgaacgt
    13321 cggtgcgact gagcgaggga tcgggcgtcc cttcgtaacg gtagtcgggg ttgaaagtcg
    13381 catccaactg cgatgtggcg cccggcgtga tggtatccgg ctcagcagtg gcaataatgg
    13441 ccggcagact ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacgtacc
    13561 ggcccccgat acaatgtcct gtaccggcga ccaggtgtaa gtcaggtttt gccccgctcc
    13621 gctgcctatt cgcaaggtag ccgtatcgcc ccgacatatt gcaacggggc cgggcggcga
    13681 ggcattgact aaaatgacgg gaaagctttg cgccgtagtt tgcacacagc cgttctcggc
    13741 ctcaacggtc agttctaatg tgtaaacccc atcgttgcga annnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnacatca aagccgggaa acaaggagtt gggcaacagc ctgatgctgc gctcggtggt
    13981 gtcggcgcat acggtgccgg ggttggcaat gagcgtgatc tggtaggtgc cggtatcgga
    14041 aaaggtaaag gtgggattga tattagtaga aaaagcgtcg ggttgggccg gatcgttgaa
    14101 gcgccagagg tattcggtgg cgttgttact ttggttttga aaannnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggact
    14401 gacccaagta acgggcagat agggcggcgg gttgggtggc tgcggcatgg ggttggcttg
    14461 gttggcgccc tggagcggtt cgcacaggcg atacacgatg gagtctccgt caatgtctct
    14521 ggcagattgg tcaaattcaa tgggtatgcc ggcacagatg taaatgggcg gccattggtt
    14581 gaactgtggg ctgctgttgc actgttgcag cgccgcttcc gaaatggtga cggaataagt
    14641 ggccccggta gctaagggat tgacaatgtt gacgatggtc tgattgcgac aacagcgttg
    14701 gtaggccagc gtgtaacctc ccgatatggg ttccaactca accgtgtccc gataaaaggt
    14761 ggtgtgaaca caaacgctgg gcggcgctac aaaacactcg ccgctgagta ccggctgaag
    14821 ggtatcgttg cccatcggcg ccagcaaaat ctgccgcagt aaaacattgg aagcattgaa
    14881 tactccaatg gacgcagggt cgtcgaacca ggctaatgga ttgccataaa agcaatcgcg
    14941 aaaaatgacc aacgtgattt cgtactcgtt gttgccgagg catttatacc tcatttcacc
    15001 gcctacaatg tgggtggctt tcagttcggc ggaaccgacc agcaacaaga gcattacaaa
    15061 gaagagagcg cgaatggatt tcatggccgt tttggaatga ccgcttgaag gtaggaggat
    15121 tttagaattt tagatgagat ttttagaatt ttcctgcggg cacagcaaca aaattggccg
    15181 gcgtatctgc acagcgcaca tacccggcca atttttgcaa aagatataaa aaccttattc
    15241 ctctatcgct ttcacgcccg gcagcacttt gccttccatg aattcgagca tggcgccgcc
    15301 gccggttgag acaaaactca cctgatcgcc gaggcccatc tggttgacgg cagccaccga
    15361 atcgccgccg ccgatgagcg aaaaagcgcc ggtagtttgg gtcgcctttg ccaccgcttc
    15421 cgcaatcgtc ttggtgccgt ttgcaaaagg cgccatttca aacacgccca tcgggccatt
    15481 ccagaagatg gttttcgatt cggcaatggc tgctgtaaaa gccgcccgtg cttccggccc
    15541 gatgtctaag cccatccatc cgtcggagat ggcattgctc ggctcaatgc gatgcgccgc
    15601 cgtttctgca aaagcatcgg caaccacgga atccatcggc aacaggagct tcacaccttt
    15661 ttctttggct ttcgcaacga ggttcatggc caagtcgagc ttgtcttcct ctaccaatga
    15721 attgccggta gcgccgccct gcgccttgaa aaacgtgtaa gccatagcgc cgccgatgac
    15781 gagcgtatcc acaaaatcgg ctaagcggtc gagcaattgt attttgtcgg aaaccttggc
    15841 gccgccgagg atggctgtca gcgggcgttc ggcgtgatgc agcacttggg cggcattttt
    15901 caattctttt tccatcagga agccaaagct cttggcgtct ttgccgaaga attttgccac
    15961 gatggtggta gatgcgtgcg cccggtgcgc cgagccgaat gcgtcgttga catacacatc
    16021 ggctaaggca gccaattgtt gggcgaaagc ctcgttgcct ttctcctctt ccgcgtggaa
    16081 acgggtgttt tccaacagca gtacctcgcc gggttggagc gcggcagcag ccgattcggc
    16141 aaccacacct acgcagtttt cagcaaaatg cacgggccgt cccagcaatt cagacaaacg
    16201 ccccaccaca tgccgcaggg tgaatttttc cacattgatg gttccgtcct ctttgagttt
    16261 ggccaatggc cgaccgaagt gcgacatgag aatggccgcg ccgccttgct ccacaatgta
    16321 gcggatggtg ggcaatgctt cgcggatgcg cgtgtcgtcg gtgatgttgt attgcttgtc
    16381 taatggcacg ttgaagtcca ctctgacaag ggcttttttg ccggagaaat tgagttgcat
    16441 gagcaaagaa ttgagctttg agctgcgagc ttcgggcgat cattcccgaa actcgcggct
    16501 cgcagttgag tgatttagat tttcactacc agatcggcca tacgggcaga gtagccggcc
    16561 tcgttgtcgt accagcccac cactttggcc atattgccgt tcaccatagt gagatcagca
    16621 tcgaaaatgc aggagtacgg gctgcccaga atatccgcag aaacgatcgg atcggtgcag
    16681 tattccagaa tgttttgcag gtaggaatcg gcagcagctt tgaaagcggc gttgatttca
    16741 tccttggtag ccggcttttt gagcagcgcc gtgaagtcgg tcacagagcc gtcgggaacc
    16801 ggaacgcgca tggccgtacc gttcagtttg cctttcaggt gcggcaacac ttcgcctacc
    16861 gctttggcgg cgccggtaga agtcggcacg atggaaacag cagcagcgcg ggcgcggcgc
    16921 aggtcgcggt gcggagcatc ctggatgttt tggtcggccg tgtaagcgtg aacggtcgtc
    16981 atcagaccca tttccacacc aaaggcatcg tcgagcactt ttaccatcgg cgcaaggcag
    17041 ttggtggtgc aggatgcgtt ggagtacatt ttgatgccgt ctttgatagc gtcgtcattg
    17101 acgcccatga ccaccgtcgg cacgttgcct tttgcaggag cagagatcag caccttttta
    17161 gcgccggcct gaaggtgcat ggcggccttg tcctcatcgg tgaaacgacc ggtacattcc
    17221 aataccacgt caatgttcat ttcgccccag ggcaatttgg tggggtcctt ttcggccagg
    17281 gcgcggatgg ccttgccatt gacgtaaatg ttgttttcgt cggcagaaac cgtaccattg
    17341 aacttgccct gtaccgtgtc gtacttcagc aggtgagcca gtgtagggtt gtcggtcagg
    17401 tcattgatgg cgaccacttc cacttccgga ttggcaatga ggtttctgaa agtaaggcgg
    17461 ccgatgcggc caaagccatt gattgcaatt ctttttgcca tggttaatga ttgatttaaa
    17521 gaggtgtttc aaatacttag tgtaaaagta acaataattt ttatttccaa catcaaaagt
    17581 tgtgttattt tattgtcacc tttttcgggg cgcaaattta aggagaagat tgcagttggg
    17641 gaattttttc ctcatccact gagttgtaga tgcgttttga aaaaaaactg aaaaaaattt
    17701 caaccaccta ttgcaaaaac taaaaacgct tgtatctttg tcccgctttt gaaaaaaagc
    17761 actacaaaac gagcaaaagg cccgttcgtc tatcggttag gactcaagat tttcattctt
    17821 gcaagagggg ttcgactccc ctacgggcta ccaaaaagac ctgtaagtca atgacttacg
    17881 ggtctttttt ttatccgccg atcaaagcat ttctgatctt cgaagccgtc tatgtttttt
    17941 catggcagcc atcccaccta ttcatctcgc cgatggtcgg cagctcctct ttataaacca
    18001 tcaaggcaga catcaaccgg agagacagga aatgcttgtt tttcaaggca gtattgttgt
    18061 tgtctgaaag acggggtttt aggggatgtg gggtggcgac ttcaatttat ctccaatgaa
    18121 catcgtcaat tttccagata ccatttactt tcaccattgt cagtatattc cttccccagt
    18181 aggtattctc ttcattttca ggattgtagt cagtatattg aatagcaaca gtggcggata
    18241 atgaattatt caacctgact gtttgaattc tgattccccc gataggttct tgcccgccca
    18301 tccaccgata ggaattgccg aaatcactcc tatggctgag cagaacatgg cggcaatcag
    18361 caagcccagg aatgggtgga tcatgtgtcg ctttttgtaa agttttgaaa acgagggaga
    18421 tttccccctt tagccgcgag atgacacctt ccacgccaca ggcgtggcag atgtcacctc
    18481 gctattttta cctccccacc gtcacccgca cgccctgaga atggctcgtg aactccggcg
    18541 catacatgca ctgtatgctg gtgatgccgt tggagaaatc gccgttgtgg gctgcgcgga
    18601 gcgggtactc gaacacataa gtgcctttcg gcagatagtc gaagaagaag tgcgtggccg
    18661 cgtcgccggt gctttcgtag taacccaagc cgccttgcca tttgtattgg ctgagtacgt
    18721 tgatgggttc aaaacccgaa gcccgcatgt ccttcatgtg aacgtactcc atgtcgcggt
    18781 ccacgcggag ttcaatgcgg acgatgagct tgtcgccggg gcgcagggcg cggttgttct
    18841 cgatggtgcg caataccgga ccggtggacg aggcttcttc gcggaagagt tgctttttca
    18901 gcgtcagcgg cgtttcctca aaggttttga tcttgtccaa atcctcgaaa tactgccagt
    18961 aagcgctgcc ccaagccacc accttgttgg ggtttttgac cttgatggag gcgagatcgg
    19021 aggagactnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgaaat agcccgttcc
    19081 ggcctgcgcg tcgttttggg ccgccgtgat gcggtcggcg tacagacgct gggtattttt
    19141 atcaaaactg acctgcacag ctttgtcggc gctgagccag ttgtcgccgt gtcccagcag
    19201 cgcgtacact gcggaggccg tagctttggt ggttttccag ttggtggtct gtttgttttt
    19261 cagcaaccag aggcgcagtt cgtccactgc tttttcgtct ttggcggcct cggcaaacac
    19321 ttcaatcatc agcgcgtgcg tctcgatggg catttggtac cagaagtagc cgcggtcgta
    19381 tttccagtac atgccgagtt cttcggaacg cagggcgcgc tcgcgcagag aagctacgat
    19441 ggaacgcgtg gccgccgcat tgccggagcg gtgcagcgcc atcgccagca gtccttgttg
    19501 gtaaatgccc ttgccgagcc agtactgagc agcctgtccg gcgtagtagt cgcgggcgcg
    19561 ttcggcgcgt ttttccagcg tgatttccgg gaagaagctg cgggcgtaga ggtagtgaat
    19621 ggctaagtgc gtgaggtggt cgtcgctgag tttggttttg ccggctttcg ccaatttttc
    19681 caactcctcg tactgccggg ccaactgctc gtcaatgtgc ttcagggctt tggtcgccac
    19741 gctcatggcc tgcgcgttgt cggcggcgct gagtgctttg agttggcgca ggtggccgag
    19801 gccctccaca acgtactgcg tgatgtacca gttttcctgt ccgccgttga accaggcaaa
    19861 accgccgctc ggcagttgcc gttgggctat tttttccaaa gcctcttttt gctctttggc
    19921 catgcggtgc aggtcgaaca gcagggcgat gttctggcgc tgttccgatt ctttttgcgc
    19981 agccagtacc cagggcgttt cctgcaagag ggcggttttc agttcctgat ttttgctcag
    20041 attactttgc agggcatcgg agcctttcca ggaatcgaac acctttttga tgttgggctg
    20101 actattggcc accgaagctg ctaaagtatt ggcataaaag cgattgaaca cctgctcgat
    20161 gcactcataa ggatactcca tgaggtaggg cagcgcctgc acggcatacc aggcgggatt
    20221 ggaggtaaat tccagcgtga gttggtggtg ctgcaaagta ggcgatttgg cgtcgcgcaa
    20281 accgctcagc gtgaaggttt tcgtttgtcc gccgcgcacc gacatgggca gggtttcggt
    20341 gaccaacatg cggttggtca gcacggggat ggagctttct tcgccgtcgc tgtaattgcc
    20401 ggcctgcgcc gtgacgcggt gcgtgagggc cattacttgt ccttgcggaa tgcggagttt
    20461 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnngcccag cagagcgtcc acgggctgca tactgagcgc gtcgaagagt tcgaggcggg
    20581 cggttcctgc cagcgtgcct gccgtgaggt tgctcacttt ggccgtaaat tcaatctcgt
    20641 cgccttcgcg cacaaaacgc ggggcattgg gcagcaccat caggtctttc tgcgtgagta
    20701 cgtcgcgggt gaagatgctg gttttcaggt cggtagtatg cgccagaccg aggaa
//
LOCUS       DAORLF010000026        20580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1399_length_20580_cov_2.266261, whole genome shotgun sequence.
ACCESSION   DAORLF010000026 DAORLF010000000
VERSION     DAORLF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20580
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_20580_cov_2.266261"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1908)
                     /locus_tag="PK198_03975"
     CDS             complement(190..1908)
                     /locus_tag="PK198_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 26 protein"
                     /protein_id="HRF37924.1"
                     /translation="MQFSKIMPILEWLPRYTKSDFRGDFQAGLTVGVMLIPQGMAYGL
                     LAGLPPIYGLYSGIVPLLLYAFFGSSRQLSVGPVALVSLLVLAGVGRFAETGTEAFIS
                     MAIATALIAGLIQVTLSIFRLGFLINFLSHPVISGFTSAAAFIIGLSQLKFLLGIEIP
                     RANRVQDIFVDAAANIHHTNFITLAIGLIGIVLILMIRRIRKTLPSGLIVVLLGTAVV
                     WLGHLDQYGVDIVKDIPRGLPAFSLPKLTMEEIGKIFPLALTICIISFIESLAIAKTL
                     ESRHKSYRVDANQELLALGMSKIGGAFFQAFPTTGSFTRSAVNDEAGARTGMSSILAT
                     VLVIITLLFFTPVFYYLPKALLASIIVAAVIGLVDYKEALHLWKTDRRDFLTLMATFL
                     ITLTLGIQTGVATGVILSIALLIYRSSKPHVAVLGRLPDTRYFRNVTRFEHAIQYPDK
                     LILRFDAQLYFGNAEFFRETVEKLSIDKGEELRYVILDCASVSDMDSTGAHTLREVVS
                     FLKTRGVVFAMASVIGPVRDTLGNTGLLDLIGKDNIHLNVYEAINYYKKKTEEASMPS
                     PTAPAP"
     assembly_gap    2260..2275
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2276..2817
                     /locus_tag="PK198_03980"
     CDS             <2276..2817
                     /locus_tag="PK198_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF37925.1"
                     /translation="QSGCGKTTLLHLLGGLLAPRSGSVQVAGTDFDSLSTAQLDAFRG
                     KNIGIIFQKSHFARALSVEENLLLAQQLAGLQPDRQRAAELLQHLDIGHKMKSRTDRL
                     SQGEQQRVAIARAVINRPKLILADEPTSALDDDNCAEVVALLEREAAQTGATLVIVTH
                     DGRLKEKFTHRISLQPQRP"
     gene            2814..>3438
                     /locus_tag="PK198_03985"
     CDS             2814..>3438
                     /locus_tag="PK198_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF37926.1"
                     /translation="MNLLQLSWKNLTFKPLSTLLNVVLFALGAGLISLLLLLDRQLEE
                     KFEKNLAGINLVIGAQGSPLQLILSGMYHIDAPTGNLPIEECKAFLRPGHPFIEKAVP
                     LSLGDSYKSYRIVGTDLNFPKLYNASLREGRLWEKTFEVCVGASAAERLGLKLGDAFH
                     SSHGFVQDDNLVHTDADKFRVVGIFNPTGAVIDQHIVTPTQSIWAVHA"
     assembly_gap    3439..3451
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3452..>3953
                     /locus_tag="PK198_03990"
     CDS             <3452..>3953
                     /locus_tag="PK198_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37927.1"
                     /translation="HSEEEDEAAREHEHAHPEFDLAAPLHEQTDRAITSILLKFKARN
                     VQTLNMQRSINENTNMQAATPAIEITRLRGLMGMGAEALRVLAFVIIGVSGLSIFISL
                     FGSLRERRYELALMRVMGASPFRLMVLILSEGVITALMGYALGMALSHFGMEIMARFM
                     QDEYRYT"
     assembly_gap    3954..3963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4098..5105
                     /gene="holA"
                     /locus_tag="PK198_03995"
     CDS             4098..5105
                     /gene="holA"
                     /locus_tag="PK198_03995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRF37928.1"
                     /translation="MSHDQILRDLKAGKVQPVYFLHGTEPYFIDLVSNYAEKELLSEA
                     EQGFNLTVLYGKDTDHLAVLDAARRYPMMAPLQVVVLKEAQDMKSLPELINYVEKPTP
                     TTALFICHKHKKFNTATKFGTALKSHAVILESKELYDNQMPAWIQDYLKSRQLKITPA
                     ALHLVIEYLGTNLSKVANELDKLALNLPAGTEVSETHIETHIGISKDYNVFELQKALG
                     ERNVQRVGRIVNYFGANPKSSPLIATIASLYNYFSRVYMLQFVPGKSDKEQAEAMQLR
                     NMYFLRDYKTAAANYPRRKLEDIIGYLHEYDLKSKGVNIDSANYEDGDLLRELVWKIL
                     H"
     assembly_gap    5220..5236
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5237..5423)
                     /locus_tag="PK198_04000"
     CDS             complement(<5237..5423)
                     /locus_tag="PK198_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRF37929.1"
                     /translation="MLVKTPPPPYYAVIFSSLRADDDPAYHETALRMVELASNQPGFL
                     GMESARNEVGITVSYWEN"
     assembly_gap    5532..5606
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5607..6026)
                     /locus_tag="PK198_04005"
     CDS             complement(<5607..6026)
                     /locus_tag="PK198_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRF37930.1"
                     /translation="MPVSIAVFASGTGSNARRIMQHFQHSDAVQVRLVVSNKADAGVL
                     EIARDMGVETLVINRTEFYHSETIVEELRRRDIRFIALAGFLWLIPEYLVQAFPRAIV
                     NIHPALLPKFGGKGMYGAHVHEAVKQAGETESGITIHY"
     gene            complement(6030..6941)
                     /locus_tag="PK198_04010"
     CDS             complement(6030..6941)
                     /locus_tag="PK198_04010"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRF37931.1"
                     /translation="MRVSSLFFAALFLLGGLGSLHAQELQFSIKINTQRLQTVDPKVF
                     ETLEKTIYDFLNNTKFTDHVYEQGERINCNIQLIIQQEISPTRFKADIAIQASRPIYG
                     SNSQTTMINHQDRDVTFDYEQFQPLIFSRNAYNDNLSAVLSFYAYFILGMDYDSFSPF
                     GGDPYYKIAQDIVNNVPQGAAVANPGWRSVDGTRNRYWMVENVLSPRVRPMRQAIYDY
                     HRHGLDVMAKQPAAGRAIMMQALDDIAQVNQVYPNSMIVQMFNQTKGQEVTEIFKGGT
                     PQEKDRVIGVLTRIDPTNAGRYRSIRS"
     gene            complement(6950..8164)
                     /gene="coaBC"
                     /locus_tag="PK198_04015"
     CDS             complement(6950..8164)
                     /gene="coaBC"
                     /locus_tag="PK198_04015"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRF37932.1"
                     /translation="MSSLQNKKIILGITGSIAAYKAAQLVRLLAKSGAEVQVLMTDAA
                     TGFITPLTLSTLSKRPVLTGIHTEEGWNNHVELGLWADALVIAPLTATTLAKLSMGIA
                     DNILAAVYLSARCPVFFAPAMDLDMWKHPAVQGHVERLQSFGNHLIPVGYGELASGLT
                     GEGRMAEPEEIVRLLEEFLAKKQDLTGKKILITAGPTYEPIDPVRFVGNRSSGRMGVA
                     LANAAARRGASVTLILGPSKLSPTEPSVETIRVETAQQMYDQALRCFPAADAAIMAAA
                     VADYRPAEVADEKIKKAGEEMNIRLVKNPDIAATLGSRKQPGQIIIGFALETNNEADN
                     AREKLLKKNMDCIVLNSLRDAGAGFDVPTNRVMLLFKDNNSLHFELKHKDAVAEDILD
                     AAVRLWNVSSTI"
     gene            complement(8233..8589)
                     /locus_tag="PK198_04020"
     CDS             complement(8233..8589)
                     /locus_tag="PK198_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRF37933.1"
                     /translation="MSDIKAKAQGLEPSVGPRDVGAMVKDTGNVYESLSIISKRSRQL
                     NTELKYELHQKLEEFAVSSDTIEEINENKEQIEISKFYEKLPNPALIATEEFTKGELA
                     YRLVDKKPRLNRGGGE"
     gene            complement(8549..9400)
                     /gene="bamD"
                     /locus_tag="PK198_04025"
     CDS             complement(8549..9400)
                     /gene="bamD"
                     /locus_tag="PK198_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRF37934.1"
                     /translation="MRKKFALPVLFAGFAAMLVFGGCRSEYEKIRTGGDPETIRKKAF
                     EYYDEKDYVKAQGLLELALPSFKGKKEAEEIYYRYTYTYYHTRQYLLASYYFNSFVQT
                     FGGSPYKEEMAFMSAYSNYKMSPTFRLDQTHTLKAIEDFQLFTNTYPESERVAECNKL
                     IDELRSKLERKAYDEGRLYFDLRNYQSSVQSFENLLRDFPETNNAEDVRFMILKANFL
                     LAENSVIDKQGDRYTETLERAEEFLARYGKSANAKEAATMKETSIKKIKQLENVGYKS
                     KSTRSGT"
     gene            complement(9444..10472)
                     /locus_tag="PK198_04030"
     CDS             complement(9444..10472)
                     /locus_tag="PK198_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF37935.1"
                     /translation="MQRFLFSLCMVCGMFVIGTAQTLPYTFSTATAVYEPLENAISLN
                     NGELWDDPDFVVPIGFEFDLFGQSINELSFDYGLGGIVGAVDNGENFISLLIPYGSDI
                     ADRGQLTGESKSEIRYLTEGAPGSRIFKLEWSNAGFYYEMDENETNDDFVNFQMWLYE
                     GSNIIEFRYGESSIADPDLVHEFAGSPLVALVDTIIADGSDVASLFYLKGDPDSPSVF
                     TTNSILDLFFLNSILDDSPADGQIYRFAPNVSSLFGPSLVASEVQAWPTVATEKVTLY
                     WPEWETTQARAQARIIDAQGRAFGSPTAIAGAVTEISVAHLPAGAYWVQLLLPGGGMA
                     GSKKIIKQ"
     gene            complement(10648..11415)
                     /locus_tag="PK198_04035"
     CDS             complement(10648..11415)
                     /locus_tag="PK198_04035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF37936.1"
                     /translation="MNKKQWYESLFENYGEKYDQESFTQGTIGECDFLEEELGRDMPL
                     RILDVGCGTGRHAIELTQRGYSVTGIDLSASQLKRAAEKAERLGLKIDFQQHDARNLP
                     FRQEFDAAIMLCEGGFPLMETDEMNFQILQNVTLALQPKAKFIFTTLNGLFPLFHSVE
                     AFCASQTEEGNATYHNNSFDLMTFRDHNITEVEDDSGNKKILDCNERYYVPSEITWLL
                     KSLGYRKIDIFGAKLGEFSRSHALTTSDFEMLVIAEK"
     gene            complement(11425..12057)
                     /locus_tag="PK198_04040"
     CDS             complement(11425..12057)
                     /locus_tag="PK198_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37937.1"
                     /translation="MIHIKASGITHLTDARYFAAKGVTWMGYCLDPASDQYIPPAKMQ
                     AIREWVDGVLTVGEMGFVSGDDLLDAVASYHLDAVQINMWVDAADLEPLQGRAEILRE
                     VVVLRDSDAAELQKEMNRMAHLITATVLSFAPQGITWADIRAGRPFGPDALRQLCSGS
                     PVLLDIDVAPADMPELLAAVQPYGWVARGGEEERVGVKSYDDLDEIFEAF"
     gene            12145..12492
                     /locus_tag="PK198_04045"
     CDS             12145..12492
                     /locus_tag="PK198_04045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRF37938.1"
                     /translation="MPAPDNYFDLVYEITRQIPRGRVSTYGAIADYLALGSARMVGWA
                     LRHSFSEYQVPAHRVVNRQGELSGRHHFPRPDMMQELLEQEGVEVHNDRVQRFEEVFW
                     HPQELDASDFFTK"
     assembly_gap    12656..12699
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12700..12944
                     /locus_tag="PK198_04050"
     CDS             <12700..12944
                     /locus_tag="PK198_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRF37939.1"
                     /translation="DTRCDKCSGAKKDQLVVGMNVVEGMAAEDGMWKGGKILDPENGN
                     EYACSMWFEKGQPDELRVRGKHWTGLYRTQTWYRVK"
     gene            13049..13285
                     /locus_tag="PK198_04055"
     CDS             13049..13285
                     /locus_tag="PK198_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37940.1"
                     /translation="MLQTCTRNDLIRFIYRETTLTENLAVRQALDEDIFLREAYEELY
                     EGYRQLPKATFSPAPSAVRNILAYSAQTALEQQY"
     gene            complement(13370..13996)
                     /locus_tag="PK198_04060"
     CDS             complement(13370..13996)
                     /locus_tag="PK198_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRF37941.1"
                     /translation="MEFFLGTLFSLFSIVDPPGALPVYVSLTGDLPQKERNKLALHTS
                     LYFLLILVAFFLAGAYILSFFGLSLYAMRIAGGMVLLSTGFGLMSGNFSKHRGYNDSV
                     KADAASRSEIAFTPMAMPLLSGPGSISYLIMQFKQHPDWNERAMILLAIVAMGGLVYL
                     TLRAAPLLFRIFGTGGLSAIARIMGFLVVAIGVQFIVDGLVHLVGAIE"
     gene            complement(14050..15720)
                     /locus_tag="PK198_04065"
     CDS             complement(14050..15720)
                     /locus_tag="PK198_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF37942.1"
                     /translation="MDKGKILEVIGLNVEFPTRQGVVQAAKDVGFVLHAGRTLGIVGE
                     SGSGKSVSALSVMRLLPAGKCRQSGQILLSGGDDAAPLPLHQLGEREMQAIRGRRIGM
                     IFQEPMSSLNPVFRCGDQITESLYRHLKLDAKTAAERTLEWFEKVQLSDPDRIFQSFP
                     HQLSGGQKQRVMIAMAMCCRPDLLIADEPTTALDVTVQKAILDLIRELQQNTGTACLF
                     ISHDLGVISELAHEVAVMHQGVVVENGPVEEVFRNPQHPYTRGLIACRPPLHKRLHRL
                     PVVSDFLSPDAEAPAPVEAAPRIVDAAAPLLDVRGVSVRYPGRPPVQALQDASFVLYS
                     GETMGIVGESGSGKTTLGRALLRLVDAHSGEAWYNEHNLLALSDASLRALRREIQMIF
                     QDPYSALNPRHTIGRAILEPMQWHGLCGSADEGREQSAELLRTVGLGPEYFDRYPHEL
                     SGGQRQRACIARALSVRPRLLICDESVSALDVSVQAQVLNLLQDLRDAQGLSMIFISH
                     DISVIRQISDRVLVMKDGRIVESGPTDEVCSQPKSDYTRQLMEAVPGK"
     gene            15744..17822
                     /locus_tag="PK198_04070"
     CDS             15744..17822
                     /locus_tag="PK198_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRF37943.1"
                     /translation="MRSCFSTFLFVVFTLLSGLPLIAQNPSLNMTELSRWNNPDLPTH
                     SGIRYNDVWGYADCSGREYAILGSAGRIHFIDVTKPEEPIEIQSFPGSVNSIWRDFKT
                     YRDRAYACADEGTDGLMVFDMSRLPDTVMMTYQNNTTFQRSHNLWVDTLQGRLYMAGT
                     NTQHNGVIIYDLTQNPDEPQLLASIPLPGGYIHDIHVVDNIAYCSHGGNGFWVYDLSD
                     PQNIILLGSMTDYPERGYNHSGWLDADRNFFIMADETHGTSVKVVDVQDFDDIYVRSL
                     FKSALLAPNLGSIIHNPFIRDQYVVMAYYHDGIQIYDMSNPDTVVRTAYYDTYPINMN
                     YGGYNGAWGAYPYLPSGNILGSDITFGLFVLRADSIQFQPGPRHLSPEAAILNVNPAL
                     CEGSPLELTAAPGAESYRWFFNGDPIHADESSYVAAEAGYYQVEARNGHCASRSDSVF
                     LELISAPALKAALDTPAFCEGDSARLGIAGLADSIFLIREADGFRELVDLSLQIFEGG
                     IYGIEAYLGACVFTLEDVFEVTVYPITSPEIDMEGPVLQCSNAGQFAHFQWHLDGSPI
                     SGADAAAWTAQQSGLYTLETVDANGCYSVSEGVQVVVSSVQEVLKTEIGLFPNPIKAG
                     QSAVVLTNLPLENSSLHLRDTYGRTIAVLPVTASKQVLDTAGLLPGVYFYEWTAGNGR
                     SLGHGKLIVQ"
     gene            18048..>19537
                     /locus_tag="PK198_04075"
     CDS             18048..>19537
                     /locus_tag="PK198_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37944.1"
                     /translation="MSKTFYLLLLAVCITTALPAQSWKKSRKSADELYQQGKYVEAAA
                     QYEEAFKKKPKNKDLAFKIAEIYYSVKDYRKAAEYYQHTKDDNAKYPMSGLKYARSLK
                     QDGRYEDAKREFQAYIDKYSGSDRAIVEEIVRVEIAGCNLAKELPARANREAEIIYTG
                     KGVNTDATEFAPIAFTNDVLYFSSTTGGKARILRSQRQGKEWGKAGAPEAFPVIQNDH
                     YCNGALSADGNRFYFTICRGQETWGPLTTRCEIFLIKRQGGSWSQPVRLPDYVNMAKV
                     TATHPAVAESNGQEFLFFASNREGGRGGMDIWFVSRDLAADDTDFSFPVNLGPTINTA
                     GDDMTPFYDAGSGMLYFASNGHPSIGGLDIFKAKGLETIWTAPENIGLPFNSPADDFF
                     FTTNADGNAGFFSSNRAFGGMKNGTRDEDIFEFTIGGRRLMVKGNAYDRESGAPLNNI
                     TASLYQVLPGNVENLLLVKDFSGGAYSFEILPGRNFKIEIQSTGYKA"
     assembly_gap    19538..19583
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19584..20007
                     /locus_tag="PK198_04080"
     CDS             <19584..20007
                     /locus_tag="PK198_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF37945.1"
                     /translation="GRPIYLDKVVEKPVDPTPPMPDKPGEVIPGPETYTAKGFGPDDQ
                     LEYTSSAPRLTGTYYKVQLAALQKYDANASSLKRVKALAQSMETEVITSQGLTRVMLA
                     NFTNREEAFKAMEQAQKKGFSGAFVVRYENGQRYGKEM"
     gene            20104..20448
                     /locus_tag="PK198_04085"
     CDS             20104..20448
                     /locus_tag="PK198_04085"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF37946.1"
                     /translation="MDACTDFIYEQEEPAQSIMLALHELLTAFPEISCKIRYKIPFYY
                     RKSWVCYLNPLKNKGVELVFIHGGRLSNVQGILDAKGRKMVAGVAFSSVDQIDADAVL
                     EILAEAILLEDA"
BASE COUNT         4871 a         5547 c         5393 g         4548 t
ORIGIN      
        1 caacagcagc agcacttttt ccattgatat ccaaaatgtg gccaatcgcc aaaacctcgc
       61 tttttactac tttgataccc aaaaggctac ggtggtggag cgctaccagt tgggcatcat
      121 tcctgtattg agctggcgga gggagtttta gcagactagg agcaacctcg gtcatcgcct
      181 tatatctcat cagggcgccg gcgcagtggg gcttggcatg gaggcctctt ccgtcttctt
      241 tttataataa ttgatagcct catacacatt gaggtggata ttgtctttgc cgatcagatc
      301 gagcaggccg gtattgccca gcgtgtcgcg caccggcccg atgacgctgg ccatggcaaa
      361 aacgacgcct ctggttttga gaaacgaaac cacttcgcgg agggtgtgcg ccccggtaga
      421 atccatatcg ctgacgctgg cgcagtccaa tatgacgtat cgaagctctt ctcctttatc
      481 aatggaaagt ttctctaccg tttcgcgaaa aaactctgca ttgccgaaat acaactgtgc
      541 atcgaagcgc aggatgagct tgtcggggta ttggatggcg tgctcaaaac gggtcacgtt
      601 gcgaaagtag cgggtatccg gcaggcggcc caatacggcg acgtgcggtt tggaactgcg
      661 gtaaatcagc agggcgatgg agagaataac gcccgttgcc accccggttt gaatgcccag
      721 cgtaagggta atgaggaaag tggccatgag ggtgaggaag tcgcgccggt cggttttcca
      781 caaatgcagc gcctctttgt agtccaccaa accgatcacc gccgccacga tgatagaggc
      841 cagcagggct ttggggaggt agtaaaacac cggagtaaaa aacaataaag taatgataac
      901 caatacggta gccaatatag acgacatacc cgttcgggcg cctgcctcgt cgttcactgc
      961 cgagcgcgtg aaactaccgg tagtgggaaa tgcctgaaaa aatgcaccgc cgattttgga
     1021 catgcccaag gccagcaatt cctggttggc atccacgcga taggatttgt ggcgggattc
     1081 cagcgttttg gcaatggcca atgattcgat aaaactgatg atacaaatcg tgagcgccag
     1141 cgggaaaatt tttccaattt cttccatcgt caatttgggc aatgaaaaag caggcagacc
     1201 gcgaggtata tctttcacga tgtccacgcc gtattggtcc agatgcccca gccaaaccac
     1261 cgctgtgccg agcaaaacga cgataagccc actgggcagc gttttgcgaa tgcgcctgat
     1321 catcaggatc agtacgatgc cgataagccc gatggcgagc gtgataaaat tggtgtggtg
     1381 aatattggcc gctgcatcca caaaaatatc ctgaactcga ttggcacggg gaatctctat
     1441 gccaagcagg aacttgagct ggctgaggcc gatgatgaat gccgccgcag aggtgaaccc
     1501 ggaaataacg ggatgcgata aaaaattgat gaggaagcct aatctgaaaa tactgagtgt
     1561 cacctgaatg aggcccgcaa tgagcgccgt agctatggcc atgctgataa atgcctctgt
     1621 gccggtttct gcaaaacgac ctactccggc cagcaccaac agcgacacca atgccaccgg
     1681 gcctaccgac aattgtcggg aactgccgaa aaaggcgtac aacaacaagg gtacaatgcc
     1741 cgaatacagg ccgtaaatag gcggcagacc tgccaaaaga ccataagcca tgccctgtgg
     1801 aatgagcatg acgcccaccg taagcccggc ctgaaaatcg cccctgaaat cggatttggt
     1861 ataacgcggc agccactcca aaatgggcat gattttactg aattgcatga tggtcaaagg
     1921 attggtaaaa caaaatcggc ggcaatttat gaataaatct tcatgtattg ggttttgggt
     1981 tcaaggtttc tgttggaaac agactttgtt tcgggtttca tccgccgtag ccttggcgaa
     2041 ggtgggtttc gagttcaagg tttcaggtgg gagccgccta ccgagacacc tcgcgcttac
     2101 cgcgccacgg cgcggctact gaaaaccgcc caccgatttt ccattttcca ttttcctgcg
     2161 tacttttgca ccatgctcaa cgctcaacaa cttcactact cctacgacgg aacgcatata
     2221 ctgcgctttc ccgacatagc ctgcgccgcc ggcgaacacn nnnnnnnnnn nnnnngccaa
     2281 tcgggctgcg gaaaaaccac cctgctgcat ctcctcggcg gactcctggc gccgcgcagc
     2341 ggttcggtac aggtggccgg cacggacttc gacagcttgt ccaccgcgca attggacgcc
     2401 tttcgcggca aaaacatcgg catcattttc cagaaatctc atttcgcgcg cgccctcagc
     2461 gtggaggaaa acctcctgct ggcacagcaa ttggcgggcc tgcaacccga ccgccaacgc
     2521 gccgcagaac tgctccagca cttggacatc gggcacaaaa tgaagtcgcg caccgaccgc
     2581 ctcagccagg gcgagcagca gcgcgtggcc atagcgcggg cagtcatcaa ccgccccaaa
     2641 ctcatcctcg ccgatgagcc tacctcggcc ttggacgacg acaattgcgc ggaggtggta
     2701 gctctgctgg agcgcgaagc cgcccaaacc ggcgccacct tggtcatcgt cacccacgac
     2761 ggccgtctca aggaaaagtt cacccatcgc atcagcctac aacctcaacg cccatgaacc
     2821 tgctgcaact aagttggaaa aatctgactt tcaagccttt gtccacgttg ctcaacgtgg
     2881 tgctcttcgc gctgggcgcc gggctgatct cgcttttgct gctgttggac cggcaattgg
     2941 aggagaaatt tgaaaaaaac ctcgccggta tcaacctcgt catcggggca caaggcagtc
     3001 ccctgcaact cattctctcc ggcatgtacc acatagacgc gcccaccggc aacctgccca
     3061 tcgaagagtg caaggcattc ctgcggcccg gccatccttt catcgaaaag gcggtgccgc
     3121 tctcgctcgg agacagctac aaaagttatc gcatcgtggg taccgacctc aatttcccca
     3181 aactctacaa cgcttccctc cgcgaaggcc gcctgtggga aaagactttc gaggtctgcg
     3241 tgggcgcttc cgctgccgag cggctgggac tgaaattggg cgatgccttt cacagttcgc
     3301 atggctttgt tcaggacgac aatctcgtgc ataccgatgc cgacaagttt cgcgtggtgg
     3361 gcattttcaa tcccaccggc gccgtcatag accaacacat cgtcacaccc acacaaagca
     3421 tctgggcggt acacgccgnn nnnnnnnnnn nccacagcga ggaagaggac gaagcggcgc
     3481 gcgagcacga acatgcgcat cccgaattcg acctggctgc tcccctgcac gagcaaaccg
     3541 accgggccat cacgtccatt ctgctcaaat tcaaggcgcg caatgtgcaa accctcaaca
     3601 tgcagcgctc catcaacgaa aacaccaaca tgcaggctgc cacgcccgcc atcgaaatca
     3661 cgcgcctgcg cggcctcatg ggcatgggcg ccgaggcatt gcgcgtgctg gctttcgtca
     3721 tcatcggcgt atcggggctg agcattttca tttcgctgtt tggttcgctg cgcgagcgcc
     3781 gttacgaact ggcgctgatg cgggtcatgg gcgcatcgcc gtttcggctc atggtactta
     3841 tcctgtcgga aggcgtcatc acggccctca tggggtatgc gctgggcatg gcactgagtc
     3901 atttcggcat ggaaatcatg gcccgtttca tgcaggatga gtaccgctac acgnnnnnnn
     3961 nnnccgggct taccttcctg caagaagaag cctggctatt ggccggcgcg ctgggcatcg
     4021 gcatcgtggc agcgctgatt ccggctttgc aggccgcacg aacggatatt tcgacgacac
     4081 tggcagaagg gtaactcatg tctcacgacc aaatacttcg cgatctcaaa gccggcaagg
     4141 tgcagcccgt gtatttttta catggaacgg agccttattt tattgatttg gtttccaact
     4201 acgccgaaaa agaactcctc agcgaggccg aacaggggtt caatctcaca gtgctgtacg
     4261 gcaaagacac cgaccacctc gccgtgctgg acgccgcccg ccgttacccc atgatggcac
     4321 ccctacaagt ggtggtactc aaggaggcgc aggacatgaa aagcctgccg gaattgatca
     4381 attatgtgga aaaacccacg cccaccacgg ctttgttcat ttgccacaaa cacaaaaaat
     4441 tcaataccgc caccaaattc ggcacagcgc tgaaatccca cgccgtcatt ctggaatcta
     4501 aagaactgta cgacaatcag atgcccgcct ggatacagga ttatctgaaa agcaggcagc
     4561 tcaaaataac gcccgcagcc ctgcacttgg tcatcgaata tctgggaacg aacctctcca
     4621 aagtggccaa cgaactcgat aaattggccc tcaacctgcc ggcgggtacc gaagtgtccg
     4681 aaacgcacat cgaaacgcac atcggcatca gcaaagacta caatgtgttt gaactgcaaa
     4741 aggccctggg cgagcgcaat gtgcagcgcg tggggcgcat tgtgaactac ttcggggcca
     4801 atcccaaaag cagcccgctc attgccacca ttgcgtcgct gtacaattat ttcagccgcg
     4861 tttacatgct gcaatttgta cccggcaaaa gcgacaaaga acaggcggag gctatgcagt
     4921 tgcgcaatat gtactttttg agggattaca aaacggctgc cgccaactac ccgcgccgca
     4981 agctcgaaga catcatcggc tacctgcacg aatacgatct caaaagcaaa ggggtgaata
     5041 ttgactccgc caattacgag gatggggatt tacttcggga gttggtttgg aaaattctac
     5101 attaaaatca accaaactca tacgcccgct ctacccggca aacccgcacg gcatacgcgc
     5161 tgtaccagct tttgcggccc gattgctgcg catccaaatg ctccgtctgc cttttccatn
     5221 nnnnnnnnnn nnnnnncgtt ttcccaataa gaaaccgtaa tgcccacttc attgcgggcc
     5281 gattccatgc ccagaaagcc cggctgattg ctcgccagtt ctaccatgcg gagggcggtt
     5341 tcgtggtaag cgggatcgtc atcggcgcgc aagctgctga aaatgacggc gtaatagggc
     5401 ggaggcggtg tttttaccaa catgattttt gttttttgta agattcaccc tactactggc
     5461 aggttcagca acaattgctc tatgacgagt ggaaaatgcc tgtgttcgag ttcgagcacg
     5521 cggcgggcga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnataa tgtatcgtga tgccgctttc cgtttcgccg
     5641 gcctgtttta ccgcctcgtg tacatgggcg ccgtacatgc ctttgccgcc gaatttgggc
     5701 aacaatgccg ggtgtatatt gacaatggcg cggggaaaag cctgcaccaa atactcagga
     5761 atcagccaca gaaaaccggc caaggcaatg aatcggatgt ctctgcggcg cagttcttct
     5821 acgatggtct cgctgtgata gaactcggtt cggttgataa ccagtgtctc cacgcccata
     5881 tcgcgcgcta tttcgaggac accggcgtcg gctttgttgg acacgaccaa gcgcacctgt
     5941 accgcatcag agtgctgaaa atgctgcatg atgcggcggg cattgctgcc ggtaccggag
     6001 gcaaaaacgg ctatggaaac aggcatggct caggaacgga tgctgcgata acgccccgca
     6061 ttggtggggt caatgcgggt gagtactccg atgacgcggt ctttctcctg gggcgtgccg
     6121 cctttaaaaa tctcggtcac ttcctggcct ttggtttggt taaacatctg tacgatcatg
     6181 ctgttgggat acacctgatt gacctgcgcg atgtcgtcca aagcctgcat cataatggcg
     6241 cgaccggcgg cgggttgctt ggccatcaca tctaaaccgt ggcggtgata atcgtaaatg
     6301 gcctggcgca tggggcgcac gcgcggcgag agcacgtttt ccaccatcca gtagcgattg
     6361 cgggtgccgt ccaccgaacg ccagccggga ttggccactg cggcgccctg gggcacattg
     6421 ttgacgatgt cctgagctat tttgtaataa gggtctccgc cgaaaggcga aaaagagtca
     6481 tagtccatgc cgaggatgaa gtaggcataa aaagacagca ccgcggagag gttgtcattg
     6541 taggcgttgc gggaaaagat gaggggttgg aattgctcgt agtcgaaggt aacgtcgcga
     6601 tcctggtggt tgatcatcgt tgtttgggaa ttgctgccat aaatgggccg ggaagcctga
     6661 atggcaatat ccgccttgaa acgggtaggc gagatttcct gctgtatgat gagctgaata
     6721 ttgcagttga tgcgctcgcc ctgctcatag acgtgatcgg tgaatttggt gttattgaga
     6781 aagtcgtaaa tggttttttc cagggtttca aacaccttgg ggtccaccgt ttgcaggcgc
     6841 tgagtattga tttttataga aaactgaagc tcctgtgcgt ggagcgaacc gagacctccc
     6901 aaaaggaaca gggctgcaaa aaaaagtgaa gaaactctca taaatacggt caaatagttg
     6961 atgaaacatt ccacaatcgg acggcagcat cgaggatgtc ttcggccacg gcgtccttgt
     7021 gttttaactc gaaatgcagg ctattattgt ctttaaacaa cagcatgacg cgattggtgg
     7081 gcacgtcgaa acccgcaccg gcatcgcgca gagaattgag gacgatgcag tccatatttt
     7141 ttttgaggag tttttcgcgg gcgttgtcgg cttcattgtt ggtttccagc gcaaagccga
     7201 tgatgatttg ccccggctgt ttgcggctgc ccagcgttgc ggcgatgtcg gggtttttga
     7261 caaggcggat gttcatttcc tcgcccgctt ttttgatttt ttcgtcggcc acctcggcag
     7321 gccggtaatc ggcgacggcg gcggccatga tggctgcgtc agcggcggga aagcagcgaa
     7381 gggcctgatc gtacatttgc tgcgccgttt ctacccgtat ggtttctacc gacggttcgg
     7441 tcggcgacaa tttggacggc cccaaaatga gggtaacgga agcccctcgc cttgctgctg
     7501 cattggccaa agccacgccc atgcgccccg atgagcgatt gcccacaaag cgcaccggat
     7561 caatgggttc gtaggtgggg cctgccgtaa tgaggatttt tttgccggtc aggtcttgtt
     7621 tttttgctaa aaactcctcc agcaatcgca cgatctcctc cggttcggcc atgcgccctt
     7681 cgccggtgag gccgctggcg agttcgccgt atcctaccgg aatgaggtga tttccaaagg
     7741 attgcaagcg ctccacatgc ccttgcacgg cagggtgttt ccacatgtct aagtccatgg
     7801 cgggcgcaaa aaagactggg caacgcgccg acagatacac cgctgcgagg atgttgtcgg
     7861 cgatgcccat agacagcttg gccaaggtgg tggcggtgag tggcgcaatg accagcgcat
     7921 cggcccaaag ccccaattcg acgtgattgt tccagccttc ttctgtgtga atgcccgtca
     7981 atacaggccg cttggaaagt gtagacaggg tgagcggagt aatgaacccc gtagctgcgt
     8041 cggtcatcag cacctgcact tctgcacctg acttggccaa caaacgcact aattgcgccg
     8101 ctttgtaggc cgcaatgctc cccgtgatgc cgaggatgat ttttttgttt tgcaaactgc
     8161 tcatggcacg agcgttgata ggaaacggcc accctgatgt atgttcagga tggccgttgc
     8221 agggtacaag ccttattcgc cgccgcctct gttcaggcga ggctttttgt caaccaaacg
     8281 gtacgccagt tcgcctttgg taaactcttc cgtagcgatg agcgccgggt tggggagctt
     8341 ttcgtaaaac ttggagattt ctatctgctc cttgttttcg ttgatttctt cgatggtatc
     8401 ggaagaaacg gcgaactcct ccaatttttg atgcaactca tacttcagct cggtattgag
     8461 ctgacgggag cgcttggaga tgatagacaa cgattcgtac acattgcccg tgtcttttac
     8521 catagcgcct acgtcgcgag ggcctacgct aggttccaga ccttgtgctt ttgcttttat
     8581 atccgacatt ttctaattgt tttatttttt taatggaagt ctctttcata gtggccgcct
     8641 ctttggcatt ggcgcttttg ccgtaacggg ccaggaattc ttcggcacgt tcgagcgtct
     8701 ccgtataacg gtcgccctgc ttgtcaatca cgctgttttc ggccagcagg aaattggcct
     8761 tcaaaatcat aaaacgcaca tcttcggcat tgttggtctc ggggaagtcg cgcaacagat
     8821 tttcaaaaga ttgcaccgaa gactgataat tgcgcaaatc aaaataaagc cgcccttcgt
     8881 cgtaggcttt gcgctccagt ttgcttctca attcgtcaat gagcttgttg cactccgcca
     8941 cccgctcgct ctccggatag gtattggtaa acaactgaaa gtcttcgatg gccttgagcg
     9001 tatgcgtctg atcaaggcga aacgtaggcg acattttgta attggaataa gccgacatga
     9061 aggccatttc ctctttgtac gggctgccgc cgaaggtttg cacgaagctg ttgaaataat
     9121 acgatgccaa caaatactgg cgagtgtgat aataggtgta ggtgtagcga taataaatct
     9181 cctcggcttc tttcttaccc ttgaagctcg gcaatgccag ttccagcaag ccctgcgctt
     9241 tcacgtagtc tttttcgtca taatactcaa aagctttttt gcggatcgtt tcgggatcgc
     9301 cgccggtgcg gattttctcg tattcgctgc gacatccgcc gaacacaagc atggccgcaa
     9361 aaccggcaaa aagaacaggc aaagcaaact tcttcctcat aagggcgcaa aggtaagtgt
     9421 tttcagtgat tccggcaaat gcctcactgt ttgattattt ttttcgatcc ggccatgccg
     9481 ccgccgggaa gcaacaattg aacccaatat gctccggccg gcaggtgtgc cacgctgatt
     9541 tcggtcacag cgcctgcgat ggcggtcggc gaaccaaacg cgcgaccctg cgcatctatt
     9601 atgcgagcct gagcgcgagc ctgcgtcgtt tcccactccg gccagtacaa agtgaccttc
     9661 tcggtagcga ctgttggcca ggcttgtacc tcggatgcaa caagagacgg gccgaacagg
     9721 gatgaaacgt tgggtgcaaa gcgataaatt tgtccgtcgg cagggctgtc gtctaaaatg
     9781 gagttaagga aaaataaatc gaggatgctg tttgtagtaa aaacagaagg gctgtcaggg
     9841 tcgcctttca gataaaaaag agaggcaacg tcggaaccgt ctgctataat ggtatctacg
     9901 agtgcgacca atgggctgcc ggcaaattca tgcacgagat cggggtcggc aatagagctt
     9961 tcgccgtagc ggaactcaat gatattgctg ccttcataga gccacatctg aaaattgaca
    10021 aaatcgtcgt tggtttcgtt ttcgtccatt tcataataaa acccggcatt gctccattcc
    10081 aatttgaaaa tacggctgcc gggggcgcct tcggtcaggt agcgaatttc agatttggat
    10141 tccccggtga gttgcccgcg gtcggctata tcggagccgt aaggaatgag cagggatatg
    10201 aaattctctc cgttatcaac tgctcccacg atgcctccca agccgtagtc aaaggacaac
    10261 tcattgatag actggccaaa taaatcgaac tcaaacccaa tgggcactac aaagtcgggg
    10321 tcgtcccaaa gctcgccatt gttgagcgag atagcgtttt ctaatggctc gtaaactgct
    10381 gtggccgttg aaaaagtgta gggcaaggtt tgtgcagttc caatgacaaa catgccgcat
    10441 accatgcaga gcgagaataa aaagcgttgc atacaaatga ttttttggtg aatgactgaa
    10501 tgtattgttg tcgagaaagc ccgcaggacc ttcacatagc aaatgtaacg gcgagactga
    10561 gaaagtgcca aaacgagact tctggtttaa cgtaagttta gtacaaaact taacaaagcg
    10621 caaaagcagt catgtcttcg ggacgtctca tttctctgca atcaccagca tctcaaaatc
    10681 ggaagtagtc agggcatggc ttcgggaaaa ctctccgagt ttggccccga aaatgtctat
    10741 ttttcggtag cccagcgatt tcagcagcca ggtaatttca gacggcacat agtatctttc
    10801 gttgcagtcc agtatttttt tgttgccgga atcatcctcc acctcggtga tgttgtgatc
    10861 ccggaacgtc atcagatcga aggagttgtt gtgataagtt gcattgcctt cttccgtctg
    10921 cgatgcgcaa aacgcctcca cagaatgaaa cagcgggaac agcccattca gggtggtaaa
    10981 aatgaacttg gccttcggtt ggagcgctag tgtcacgttt tgcaaaattt gaaaattcat
    11041 ctcgtcggtc tccatcagcg ggaaaccgcc ctcgcagagc atgatggccg catcgaattc
    11101 ttgccggaaa ggcagatttc tggcgtcgtg ctgctgaaag tcaattttca atcccaggcg
    11161 ctcggctttt tcagcggcgc gcttcaattg ggaggccgaa aggtcaatcc ctgtcactga
    11221 gtagccccgc tgcgtcagtt caatggcgtg cctgcctgtg ccgcagccca catccagaat
    11281 ccggagaggc atatcgcggc ccagctcttc ttccaaaaaa tcgcactccc ctatcgtgcc
    11341 ctgcgtgaaa ctctcctgat cgtatttttc cccgtagttc tcgaagaggg attcatacca
    11401 ttgtttcttg ttcatgattt acttttaaaa cgcctcaaaa atctcatcca gatcatcata
    11461 agatttgacg cccacgcgct cctcctcgcc gccccgcgcc acccaaccat aaggctgcac
    11521 ggcggccaac agttccggca tgtcggcggg cgccacgtct atatcaagca acaccggact
    11581 gcccgaacac agttgccgca gcgcatccgg cccgaaaggt cgtccggccc gaatatccgc
    11641 ccaggtgatg ccctgcggag caaaagacag cacggtggcg gtaatgagat gggccatccg
    11701 gttcatttct ttttgcaatt cggcggcatc ggaatcgcgc agcaccacca cctcccgcag
    11761 aatctcggcc cggccttgca gcggttccag atcagcagcg tccacccaca tatttatctg
    11821 tacggcatcg aggtggtaac ttgccacagc atccaacagg tcgtcgccgg agacaaagcc
    11881 catttcgccc acagtgagta cgccgtccac ccactcgcgg atggcctgca ttttggcggg
    11941 cggaatgtat tggtcggaag caggatcgag gcagtagccc atccaggtga cgcctttggc
    12001 ggcgaagtaa cgggcgtcgg taaggtgcgt tatgccggaa gctttgatgt ggatcacgtt
    12061 gtgcaggttg gtgatatttc gacaggcgct tggtggttct gccgaaacac atatctttgt
    12121 atcgccgaca aaattagtac tttcatgcct gcccccgaca actattttga ccttgtgtat
    12181 gaaattaccc gccagattcc tcgcggcagg gtgagcacct atggcgccat tgccgactac
    12241 ctggcgctcg gttcggcgcg gatggtgggc tgggcgttga ggcatagttt tagcgaatac
    12301 caagtgcccg cgcatcgggt agtaaacagg cagggcgagt tgagcggacg gcatcatttc
    12361 ccccggcccg atatgatgca agaactgttg gagcaggaag gcgttgaagt acacaatgac
    12421 cgggtgcagc ggtttgagga ggtgttctgg catccgcagg aattggacgc aagcgatttt
    12481 ttcaccaaat aaaaacacaa tcaaacatga atttttggaa aaccgccgcc ttgatggcgt
    12541 gcctgacttt gttggcgcct tggatggccg ccgctcaatc tcccgtcgga atgtggaaaa
    12601 ccatagacga caaaaccggc gatgccaagt cgcacctcga aatttatgag aagggnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgacacccgc tgcgacaaat
    12721 gctcgggcgc caagaaagac caactcgtcg tgggcatgaa tgtagtggaa ggcatggcgg
    12781 ctgaagacgg catgtggaaa ggcggcaaaa tcctcgaccc ggaaaacggc aatgagtacg
    12841 cttgcagcat gtggtttgaa aaaggccaac ccgatgaact gcgcgtgcgc ggcaaacact
    12901 ggacgggctt gtaccgcacc caaacctggt atcgggtaaa ataaggccgc caaactttaa
    12961 cactcgccaa acaataaccg gagcaggggc catgttaaat aggcattgat tacgaaaaca
    13021 tcctgtcagc taatttaaaa ttgctcctat gcttcaaact tgtacacgca acgatctcat
    13081 ccgtttcatc taccgtgaaa ctaccctcac agaaaacctc gccgtgcgtc aggcattgga
    13141 cgaagacatt tttctgagag aagcctacga agaactctac gaaggctatc gccaactgcc
    13201 caaagctact ttcagcccgg cgccttcggc agtgcgcaac atattggctt acagtgctca
    13261 aaccgcgctc gaacagcaat attgaggccc gtttttctaa ggtatggaaa agccgctccg
    13321 atgcaaaatc agagcggctt ttctgttgga gcttgctggt acaacgcagt tactcaatgg
    13381 ccccaaccaa atgcactaag ccatctacga tgaactgcac cccgatggcc actaccaaaa
    13441 aacccatgat gcgggcaatg gcactgaggc cgcccgtgcc gaatatgcga aacagcagcg
    13501 gcgcggcgcg cagggtgaga tacaccaagc cccccatagc gacaatggcc agcagaatca
    13561 tggcccgctc attccagtca ggatgctgtt tgaactgcat gatgaggtag gaaatagacc
    13621 ccggccccga cagcaacggc atggccatag gcgtgaaggc aatctcggag cggctggcgg
    13681 catcggcctt gacggagtcg ttatagcccc ggtgtttgga aaaattgccg ctcatcagtc
    13741 cgaagcccgt actcagcagc accatgccgc cggcaatacg catggcatac agcgaaaggc
    13801 cgaaaaaact gagaatgtag gcgccggcca aaaagaaagc caccaaaatg agcagaaagt
    13861 agaggctggt gtgcagggcc aatttgttgc gctctttttg cggcaagtcg ccggtgagcg
    13921 aaacatacac cggcaatgcg cccggcggat ccactatgga gaacagagaa aacaaggtac
    13981 ccagaaagaa ttccataaga tgttcaaggt tttgaggtga caaagttaga atgatttcct
    14041 attccctcac tactttcccg gcacggcctc catcaactgc cgggtgtaat cagatttggg
    14101 ttgggaacac acttcatccg tagggccgga ctccacaatg cggccgtctt tcattaccaa
    14161 cacgcggtcg ctgatctggc ggatgaccga aatatcgtgc gaaataaaaa tcatgctcaa
    14221 gccctgtgca tcgcggaggt cctgtaaaag attgagtacc tgagcctgca ccgaaacatc
    14281 aagcgcagaa accgattcat cgcatatcaa cagccgggga cgcaccgata atgcccgcgc
    14341 tatgcaggcc cgctgacgct gcccgcccga caactcgtgc gggtagcggt cgaagtactc
    14401 cggccccaaa ccaactgtgc ggagcagttc ggcactttgt tccctgccct catctgcact
    14461 gccgcagagg ccatgccatt gcatcggctc cagaatggcc cggcctatgg tgtggcgcgg
    14521 gttgagtgcc gagtaggggt cttgaaaaat catctgtatt tcgcggcgga gggcgcgcag
    14581 tgacgcatcg gacagggcca ggaggttgtg ctcattgtac catgcctcgc ccgaatgagc
    14641 atccaccaag cggagcaagg cccggcccag cgtggttttt ccgctgcccg actcgcccac
    14701 aatgcccatc gtttcgccgg agtacaacac aaacgacgca tcttggaggg cttgtaccgg
    14761 tggccggccg gggtagcgaa cagacacgcc gcgcacatcg agcagcggcg cggcagcgtc
    14821 aacaatgcgg ggagcggcct ccactggagc gggagcctcc gcatcaggcg acaaaaaatc
    14881 agacacaacc ggcaggcgat gcagcctttt gtgcaaaggc gggcggcagg caatcaggcc
    14941 ccgggtgtat ggatgctgtg gattgcgaaa gacttcctcc accgggccgt tttctaccac
    15001 gacaccctgg tgcataacgg ccacctcgtg cgccagttcg ctgatgacgc ccagatcgtg
    15061 agaaatgaac aggcaggccg ttccggtgtt ttgttgcaac tcccgaatga ggtcgagaat
    15121 ggctttttgc accgtcacat ccaaagcggt ggtaggttcg tcggcaatga gcaggtcggg
    15181 ccggcagcac atcgccatgg caatcattac gcgctgcttt tggccgcccg acagttggtg
    15241 cgggaaagat tggaatatgc ggtcggggtc ggaaagttgt actttttcaa accactccag
    15301 ggtgcgttca gcggcggtct tcgcgtccaa tttcaggtgc cggtacaaag attcggtaat
    15361 ctgatcgcca cacctgaaaa ccggattgag cgacgacatg ggttcttgaa aaatcatgcc
    15421 gatacgacgc ccccgaatgg cctgcatttc ccgttcgccg agctgatgca agggcaaggg
    15481 tgccgcatca tcgccgcccg acagcaaaat ctgcccgctt tgacggcatt tgccggcagg
    15541 caacaaacgc atgaccgaca gtgccgacac ggatttccca ctgccggact cgcccacaat
    15601 gcccagcgtg cgcccggcgt gcagcacaaa accgacatct ttcgcagcct gcacgacacc
    15661 ctgacgagtt gggaattcta cattcaggcc aattacttcg agaattttac ctttgtccat
    15721 cgaacaacaa aaatacaact ttcatgcgta gctgcttttc tacttttctc tttgtcgttt
    15781 ttacactgct ttcgggactc cccctgatcg ctcagaatcc gagcctgaat atgacggaac
    15841 tgagccgttg gaacaatccc gacctgccca cccactccgg catccgatac aatgacgtat
    15901 ggggctatgc cgactgctcc ggcagggaat atgccatact cggatcggcg gggcgcattc
    15961 attttattga cgttaccaaa ccggaagaac ctattgaaat tcagtcattt ccggggtcgg
    16021 tcaattctat ctggcgggat tttaaaacct atcgcgaccg cgcttacgct tgcgcagatg
    16081 agggtacaga cggccttatg gtattcgata tgagccgttt accggacacg gtgatgatga
    16141 cttaccaaaa caataccaca tttcagcgca gccacaatct ttgggtggac acgctacagg
    16201 gccgcctgta catggccggc accaatacgc aacacaatgg cgttatcatt tatgacctca
    16261 ctcaaaaccc cgatgagccg caattgctgg cctccattcc gctgcccggc ggttacatac
    16321 acgatattca tgttgttgac aacatcgctt attgttcgca cggcggcaac ggcttctggg
    16381 tatatgatct cagcgacccg caaaacatca tattgctcgg ctccatgacc gattaccccg
    16441 aaaggggcta caaccactcc ggctggctcg atgccgaccg aaattttttc atcatggcag
    16501 atgaaacgca cggcaccagc gtcaaagtgg tagatgtgca ggattttgac gacatctatg
    16561 tgcgctcgct gttcaagtcg gctctgctgg ctcccaatct gggcagcatc atacacaatc
    16621 ccttcatccg cgaccagtac gtcgtcatgg cttattacca cgacggcatc caaatttacg
    16681 atatgtcgaa ccccgacacg gtggtgcgta cagcttatta cgatacctac cccatcaaca
    16741 tgaattacgg cgggtacaac ggcgcctggg gagcatatcc gtatctgcct tccggcaata
    16801 tcctcggctc cgacatcact ttcggcttgt tcgttttgcg cgccgacagc attcagttcc
    16861 agcccggccc gcgccacctc tctccggagg cggccatttt gaatgtaaac ccggcattgt
    16921 gcgagggttc gccccttgaa ctaaccgctg cgcccggcgc ggaatcctac cgatggttct
    16981 tcaacggcga ccccattcat gccgacgaaa gctcgtatgt ggctgccgag gccggctatt
    17041 atcaggtaga agcgcgaaac ggccactgcg caagtcgctc agattcagta ttcttagagc
    17101 ttatttccgc tccggcgctc aaagcagcgc tcgatacgcc cgcattttgc gagggagatt
    17161 cggcccggct cggcattgca ggccttgccg attcgatttt cctgattcgg gaggctgatg
    17221 gattccgcga gttggtggac ttgagcctgc aaattttcga gggtggtatt tatggaatag
    17281 aggcgtacct cggcgcctgt gtttttaccc tggaagatgt ttttgaagtc acggtttacc
    17341 ctattaccag cccggagata gacatggaag gcccggtgct acaatgctcc aatgccggtc
    17401 aatttgctca ttttcagtgg catttggacg gaagtcccat atccggggcc gatgccgcag
    17461 cctggacggc acagcagagc ggcctttaca cgctggaaac ggtagatgcg aatggctgtt
    17521 actcggtttc cgaaggcgtt caggtggttg tttcgtctgt gcaggaggtt ttgaaaaccg
    17581 aaatcggcct gtttcccaac ccgataaagg ccgggcaaag cgctgtcgtt ttgaccaatc
    17641 tgcccttaga aaacagtagc ttgcacctcc gcgacactta tggacgcacc attgcagtgc
    17701 tgccggtaac cgcatctaag caagttttgg ataccgcggg tttgttgccg ggcgtctatt
    17761 tttacgaatg gacagccgga aacggccgca gcctcggaca cggtaaacta atagtgcagt
    17821 aagcgctgcc attagagcgc gcatattgac gccatactga aaagggcttc cggatattcg
    17881 gatggccctt ttctgcattt tggcagcatg ataactatca cccctcgcca aacaatcttt
    17941 atctttaccc aaacgttagt cagggtcgaa agacgcacag gttctcggcc aggcgtctca
    18001 cacactggtt aaccagcttt tttcatttcg cactcaacct tcttattatg agcaaaacgt
    18061 tttatttact actgcttgcc gtgtgcataa ctacggcgct gccggctcaa agctggaaaa
    18121 aatcgcgcaa atccgcggac gaactctacc agcagggcaa atacgtcgaa gcggcggccc
    18181 aatacgaaga ggctttcaaa aagaagccca agaacaaaga cctggctttt aaaattgccg
    18241 aaatttacta ctccgtcaaa gactaccgca aggctgccga gtactaccag cacaccaaag
    18301 acgacaatgc gaagtacccc atgtcggggc tgaagtatgc ccgcagcctc aagcaggacg
    18361 gccgctacga agacgccaaa cgcgagtttc aggcttacat tgacaaatac agcggctccg
    18421 accgggccat cgtggaagaa attgtccgcg tagaaattgc gggctgcaac ttagccaaag
    18481 aactgccggc ccgcgccaac cgggaagctg aaatcattta caccggtaaa ggcgtaaaca
    18541 cggatgctac ggagtttgcc cccatcgcgt tcaccaatga cgtattgtat ttttcctcca
    18601 ctaccggcgg caaagcgcgc attctgcgtt cgcagcgcca gggcaaggaa tggggcaaag
    18661 ccggcgcgcc ggaagccttt cccgtcatcc aaaacgatca ctactgcaac ggcgcccttt
    18721 ctgccgacgg caaccggttc tatttcacca tttgccgcgg ccaggaaacc tggggacctc
    18781 tgaccacccg ctgtgaaatt ttccttatca agcgtcaggg aggctcctgg tcgcagcccg
    18841 ttcgtctgcc cgattatgta aatatggcca aagtgacggc tacgcacccc gcagtggcag
    18901 agagcaacgg ccaggaattc ctgttttttg cctccaaccg cgagggcggc agaggcggta
    18961 tggacatctg gttcgtctcc cgcgatctgg ccgccgacga taccgacttc tccttccccg
    19021 tcaacctcgg tccgaccatc aatacggccg gcgacgatat gacgccattt tatgatgcgg
    19081 gttccgggat gctctacttc gcctccaatg gccatccttc catcggcgga ttggacattt
    19141 tcaaagccaa aggtctggaa accatctgga cggctcctga aaacatcggc ctgcctttca
    19201 actcgcccgc tgatgacttt ttcttcacca ccaacgccga cggcaatgcg ggatttttct
    19261 cttccaaccg cgcttttggc ggcatgaaga acggcacccg cgacgaagat atctttgaat
    19321 ttaccatcgg tggtcgtcgc ctcatggtca aaggcaatgc ttacgaccgc gaatcgggcg
    19381 cgcccttgaa caacatcacg gcatcgctgt atcaggtgtt gccgggcaac gttgaaaacc
    19441 tgctgttggt gaaggatttc agcggcggag cttattcttt tgagatactg ccgggtcgca
    19501 attttaaaat tgaaatccaa agcacggggt acaaagcnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnntggccgt ccgatctatt tagacaaagt agtggaaaag
    19621 cccgtggatc cgaccccgcc catgccggat aaacccggcg aggtgatccc cggcccggaa
    19681 acctatacgg caaagggctt cggccccgac gaccagttgg aatacaccag ttctgcaccg
    19741 cgactgacgg gcacttatta caaagtgcaa ttggctgcct tgcagaaata cgacgccaac
    19801 gcctccagtt taaaacgcgt gaaagcgctg gctcaaagta tggaaaccga agtcatcact
    19861 tctcagggcc tcacccgcgt gatgctggcc aatttcacca accgcgaaga agctttcaaa
    19921 gcaatggagc aggcacagaa gaagggcttc tccggcgctt ttgtggtgcg gtatgaaaac
    19981 ggtcaacgct acggcaagga aatgtagggg gcatcataac gatcaaaaag gctctgggaa
    20041 aaatgcttcc cggagccttt tttgttggta aacatgtaat ttgcgtaccc aaaaatcaac
    20101 tttatggatg cctgtacaga tttcatttac gaacaggaag aaccggcgca atccatcatg
    20161 ctggccttgc acgaattgct gacggccttt ccagagatca gttgcaaaat ccggtacaag
    20221 attccttttt attacagaaa gagctgggtt tgttatctca acccgttgaa aaacaaaggg
    20281 gtggaattgg tttttattca tggcggccgc ttgtcgaatg tgcaggggat attggatgcg
    20341 aaaggccgca aaatggtggc cggggttgcg ttttcatccg ttgaccaaat tgatgcagat
    20401 gcggtgttgg aaattctggc ggaagccatc ctgctggaag acgcatgagg ggcatcaaaa
    20461 aatggtaacc attcagtcgc cctgcaaaaa gctgcgacca cgccacaggc gtggttcgag
    20521 tcgcgagttt gattttccaa aatgaaaaaa ggccattttt caagatgaga ttttgaaatt
//
LOCUS       DAORLF010000027        20231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1431_length_20231_cov_1.966842, whole genome shotgun sequence.
ACCESSION   DAORLF010000027 DAORLF010000000
VERSION     DAORLF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_20231_cov_1.966842"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    240..267
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            749..1288
                     /locus_tag="PK198_04090"
     CDS             749..1288
                     /locus_tag="PK198_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF37947.1"
                     /translation="MRKFSLFLVALFTLTLALSASAQQDKTQRPSPPMTASSSFGDTK
                     LVINYGSPAVKGREIWGKLVPYGKVWRTGANEATTFDVSTDVMIEGKKLAAGRYALFT
                     IPGEDEWTIIFNKTADQWGAYGYKAEDDALRVVVKPASHGETAERLVFHVIPDGNTKA
                     TVTLYWEKVAVGFNMTNVK"
     gene            1417..2409
                     /gene="ftsY"
                     /locus_tag="PK198_04095"
     CDS             1417..2409
                     /gene="ftsY"
                     /locus_tag="PK198_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRF37948.1"
                     /translation="MPLFLHHLNGLKVSFFSKFFNKEKKEDLDKGLEKTKTNFLGKLA
                     KAVAGKSTVDEEVLDEVENILISSDVGLDTTVKIIERIEARVARDKYLNASELSEILR
                     DEIVQLLSDNNTQDLEDFLPSVQAKPLILMVVGVNGVGKTTTIGKLAWQFKQQGKKVV
                     MGAADTFRAAAVDQLKIWSERVGCDFFSKGMNTDPAAVAYETVQYAIQNNCDVAIIDT
                     AGRLHTKINLMKELGKIKSSITKRMDGAPHEVLLVLDATTGQNAIEQAKHFTQATEVT
                     ALALTKLDGTAKGGVAIGISDQFKIPIKYIGVGEAIDKLQIFNKRAFVDSLFEK"
     gene            2532..2987
                     /locus_tag="PK198_04100"
     CDS             2532..2987
                     /locus_tag="PK198_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRF37949.1"
                     /translation="MFGNSKKDTGKPGAPGTAPSNSLNSLTQGAVVEGTVKADSDIRV
                     DGKIKGKLHCAAKVIIGPSGHIEGEIRCKNAVIEGKFIGNIDVEELLNVRETAHIDGE
                     VNVGKLIVQSGATFNVTCNMGGVKKSDQGAAGSSKANTAHASAGKPQQA"
     gene            2956..3216
                     /locus_tag="PK198_04105"
     CDS             2956..3216
                     /locus_tag="PK198_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRF37950.1"
                     /translation="MPPQENHSKPEPEKPDEQRASRARNYMKYSGMAFQMGGIILFGV
                     WVGKKLDERFSTSTPWFTVGLALFSVFAALYIVLKDFMKTPS"
     gene            3213..3638
                     /locus_tag="PK198_04110"
     CDS             3213..3638
                     /locus_tag="PK198_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37951.1"
                     /translation="MTHRSFLLQLTLLTAASAAGLWLMLGMLLLALLHAPLMLGMPQL
                     AGFEWFGWGCLIFFVLLTIAMFYAGHSAARSSNKNDFTNAAMGFSGGKIFLSAIIILM
                     YVELAAPTSKFFILPFFALYIIYTVFEVYFMSKLGRMKP"
     gene            3710..>3897
                     /locus_tag="PK198_04115"
     CDS             3710..>3897
                     /locus_tag="PK198_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37952.1"
                     /translation="MEHGPYKAIVNGAFEYEDLSPAQLDAASSGKGKHHILNGPVSLH
                     AEILETDFFQKRYVIKVNG"
     assembly_gap    3898..3933
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3934..4199
                     /locus_tag="PK198_04120"
     CDS             <3934..4199
                     /locus_tag="PK198_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRF37953.1"
                     /translation="MDQLVNRLGLSIKTTHRVKDIKAPMPGLVRRIEVSPGQEIKEGD
                     PILILEAMKMENVLKSPGEGRIAKVLVSEGAPVEKGQVLIEVE"
     assembly_gap    4252..4285
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4286..>4570)
                     /locus_tag="PK198_04125"
     CDS             complement(<4286..>4570)
                     /locus_tag="PK198_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37954.1"
                     /translation="VTQVVTRPSGCRDTATQIVDIRPEVRFHLPNAFTPNADGLNDTY
                     KGVGIMEGARAFNMSIWNRWGEMVFETDSPNEGWNGRKFNTGQDSPPGVYV"
     assembly_gap    4571..4636
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4637..>5297)
                     /locus_tag="PK198_04130"
     CDS             complement(<4637..>5297)
                     /locus_tag="PK198_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF37955.1"
                     /translation="NCGDTARVTINVFPSISADFSFAYDTCVAGPVQFTDLSMSGSGN
                     IVAWDWQFGDGQRSSARNPAHTYRIPGNIPVTLTVTDVNGCQASITKVVPYFPVPSLI
                     VIAPSSFTGCPAEIFFDNLSFPIDETYDILWEFGDGATGTAISPTHTYTETGVYTVSV
                     RIISPIGCQTDTVFNSLIRVLPSPVAGFEYTPDDPTSINNTVIFSDRSEGANRWFWDF
                     GN"
     assembly_gap    5298..5337
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5338..5700)
                     /locus_tag="PK198_04135"
     CDS             complement(<5338..5700)
                     /locus_tag="PK198_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37956.1"
                     /translation="MAGNPVVTIDSQTGLISGTPVNLGQYVVGVQVLEYRNGVLLSTV
                     FRDFQFNVARCDPTVVAQIQSDSIADGQSYFINACGDTAVTIVNTSFQQAFVTNFLWR
                     FNISGNTVTSNSWKPTINF"
     assembly_gap    5715..5842
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5843..6381)
                     /locus_tag="PK198_04140"
     CDS             complement(<5843..6381)
                     /locus_tag="PK198_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37957.1"
                     /translation="MTYRCISPGNYEFTLKMYRDCNCTDCANFDPTAFVGVYRCPGGN
                     CSGQSQGAPFRRVDVPLGSVRQVTAPSYPCLIPPNVCVQEAEYVFNLSLPVSTTDSYH
                     ITYQRCCRNETINNIFAPSTVGATYTVEITSAAQQLCNNSPTFNTFPPTVICGGLPLN
                     YDHAATDADGDSLVYRFSAP"
     assembly_gap    6391..6400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6801..6985
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6986..7467)
                     /locus_tag="PK198_04145"
     CDS             complement(<6986..7467)
                     /locus_tag="PK198_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37958.1"
                     /translation="MRYIVLFFLFFFSFNLLSAQDLSAYERRLYINADGDSLPYRILF
                     TEGYAAGKSYPLILVLHGAGERGNDNEMQLVHGAKMFLKPEVRAKYPAIVVFPQCPKD
                     LYWARVERDPERIDWAFPLFETPGKPMRLVMELVDMLMNDTKTDASRMYVGGLSMGGM
                     G"
     assembly_gap    7636..7765
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7766..>8786)
                     /locus_tag="PK198_04150"
     CDS             complement(<7766..>8786)
                     /locus_tag="PK198_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF37959.1"
                     /translation="EVEAGGRIFRDTAACTVHEIALSGLAPATEYKYTLRYGDGRQHF
                     SFRTAPRPGSRTAFSFTYASDSRAGQGGGERNVWGANHYIMKKIMALGQMKQARFMQF
                     TGDLVNGYLNNTDNMHLQYANWKRAVQPFTHYVPIYAGMGNHEALVHRFVHKTPTDSI
                     AFLIDRFPYETESAEAVFAANFVNPLNGPDSEDGAPYDPNPSQTDFPSYKENVYFYTY
                     DNVAVIVLNSDYWYAPTTARLPHTSGGMHGYIMDMQLKWLEKTVADLERNKAVDHIFV
                     TTHTPFFPNGGHVSDDMWYRGNNAMRPYVAGKPMEKGIIERRDQMLDLLVNKSKKVRA
                     ILTGDE"
     assembly_gap    8787..8876
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8877..>9162)
                     /locus_tag="PK198_04155"
     CDS             complement(<8877..>9162)
                     /locus_tag="PK198_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37960.1"
                     /translation="GAGFNGKLYYGFIPHGDARHPHPVYFRAPVNIVNGKAAIRIIGM
                     SGSYDMVGWQKNERGVVGYRVTDDTGQMFYDGIVEFRGKGPFEVAPTIVEG"
     assembly_gap    9163..9292
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9591..9612
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9613..10214)
                     /locus_tag="PK198_04160"
     CDS             complement(<9613..10214)
                     /locus_tag="PK198_04160"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRF37961.1"
                     /translation="MSLIQMVRHLRSGEQAPVNVVLTNERLPENVAAKIARFIEPDSA
                     QIYVLFSDPEYLQSIGYTPETFMSLFIPNTYQVYWNSTPEQFTERMVKEHKAFWDKEN
                     RLEKARRLDMTPEEVYTLASIVDRETLQRSEKPTIAGAYLNRLKINMPLQADPTCVFA
                     TRDFETKRVTEYHTKFDSPYNTYMYAGLPPGPIGMASISG"
     assembly_gap    10236..10256
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10257..10495)
                     /locus_tag="PK198_04165"
     CDS             complement(<10257..10495)
                     /locus_tag="PK198_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37962.1"
                     /translation="MQRSLLRSLLLLLLLAGGVAAFVYWRYVAKTAVPRGLDDYHLLV
                     PTGANFDQVTDSLLAKGFIRDAAAFRVIAEQMKYKR"
     gene            complement(10502..11317)
                     /gene="panB"
                     /locus_tag="PK198_04170"
     CDS             complement(10502..11317)
                     /gene="panB"
                     /locus_tag="PK198_04170"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRF37963.1"
                     /translation="MSVHKEVKRVTTHVLQAMKARGEKIAMLTAYDYSMARILDEAGT
                     DILLVGDSASNVMAGHETTLPITLDQMIYHAASVVRAVKRSLVVVDLPFGSYQGNSTV
                     ALKSTIRIMKEAGAHAVKLEGGEEIAESVARILSAGVPVMGHLGLTPQSIYKFGTYTV
                     RAKEEAEAEKLLSDAKLLQELGCFAIVLEKIPAQLTRRVSEELIIPTIGIGGGPSADG
                     QVLVTQDMLGITKDFQPRFLRRYLELYDLIRGATEQYIEDVKSQSFPNEKEAY"
     gene            complement(11355..12857)
                     /locus_tag="PK198_04175"
     CDS             complement(11355..12857)
                     /locus_tag="PK198_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37964.1"
                     /translation="MQQIYSETSKVFIFSLFGIACLLSLFSCDPATERISGNTNDDAM
                     EANRPYLERLNDQGDSATAAGDYAAAITFYQKAMEIAAAEGDSFPYYDAQLDLACVHE
                     RLREFPKAIELGEDALAAFIRSGDSTRIGRAYSTLSSFYGLAEIPEKQMATAQKGFEI
                     LKNYGSLIERCAAYNQMAFTYSGAGDWAKALPLLDSALLLMKASGYLDQLPGLLLNIG
                     DCHRNLGHYEAAKKYLKEAAAGCDSLGYVHIHARAVQLLSMSEESTGNIAEALQLYKT
                     FKILRDSVINEEKNNTIQQLKIEYETEEKERENQLLKARQQIEVARRNMALALWGVTI
                     SAIGLWLYFRREKARRLQAELAQNRQQLEEFAQLLLAKNTRLMELEQNQERPTESAEP
                     EGDTAESDTPESVALYDIHILTEKDWTAFKRSFERSYPGFIHRLRTHYSDITNAEERL
                     FLLYKLNFTRHEIATILGVSEASVKKGRTRLRKRLGLQEEQDLEHFIQSF"
     gene            13134..14648
                     /gene="purH"
                     /locus_tag="PK198_04180"
     CDS             13134..14648
                     /gene="purH"
                     /locus_tag="PK198_04180"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRF37965.1"
                     /translation="MIRSALVSVYYKDGLEPILRELHRHGVTLYSTGGTQQFIESLGL
                     SATAVEDITGYPSILDGRVKTLHPAVFGGILARREPEHLNQLQQYSLPEIDLVIVDLY
                     PFEETVAGSASEQDIIEKIDIGGISLIRAAAKNFGDVAVVASRQEYGLFLEMLQSGNG
                     SLSRDQRRELARRAFKVSSNYDIAIFNYFNEGTTDIAFKYSIHRSDILRYGENPHQSG
                     VFFGDFDQIFDKIAGKAISYNNLVDIDAAVQIMRELRNTEPAFAILKHTNTCGLALRD
                     TLPEAWRAALACDPVSAFGGIFICNRPVDLETAGEIDKLFYEVLIAPGFDEDALALLQ
                     AKKNRILLLIKDFYVRPRSFRSLLNGVVEQDTDLRQDTEDDYQTATLRAPSASEVSDL
                     LFAGTAVKHLKSNGIALVKNKQLIGMGCGQTSRVDALRQAIAKAAAFGFDLKGAVMAS
                     DAFFPFPDCVEIAHEAGITAVVQPGGSVKDQDSIDFCNAHNMAMVLTGVRHFKH"
     gene            14708..15433
                     /locus_tag="PK198_04185"
     CDS             14708..15433
                     /locus_tag="PK198_04185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRF37966.1"
                     /translation="MSNLVIDIGNTRTKAAVFRNDEMTERLMWEQCTREEISRVLANH
                     RVENVILSVVGPPMEETLRHWLVAQVRFFELTPALHLPFRNLYGTPATLGKDRVAAVA
                     GALAMFPGRNCLVIDAGTCITYDVLTAEGAYLGGNISPGIAMRFKALPTFTAALPLVE
                     RGPMQDAVGSSTETAIRNGVQYGVLWETEGMIAAMQQRLNDLNVVLTGGDADFFVKNL
                     KSRIFANANLVLEGLNKILNYNV"
     gene            15426..>15946
                     /locus_tag="PK198_04190"
     CDS             15426..>15946
                     /locus_tag="PK198_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37967.1"
                     /translation="MFENLKRVVLGAILCVPAFALLNAQTADIEPRSNSPYSRFGLGD
                     IVNPLFAAQSGMGGLSAAYRDPFHLNMANPAALGHLMATDLETGFFAKYSSLSDGSTS
                     ASLWSGNLNYFALGMPIINPINRALEKKSDKIGLGMGFVLQPYSNVGYNLVTTQELPE
                     IGEAVTSFKGSGGA"
     assembly_gap    15947..15987
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15988..16788
                     /locus_tag="PK198_04195"
     CDS             <15988..16788
                     /locus_tag="PK198_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37968.1"
                     /translation="GRFSAGVNLGYLFGKMSFDRTVEFSDVANQAYLTRYLDEQSVSG
                     FLWNAGAQVSIPLEKKPEGVGRSTKEKNLILGLYGNSSTNLNTNNSRFHTRYNQTYND
                     RDTILLETDSIRKGTLPGELVFGITYDQSDKLKLGFEYALGGWSAYANPLQGQTLSNT
                     RRISAGIEYVPDMFSYNNYWQRVRYRGGIYHRTDPRSVNGKQLTEYGISAGLGFPVIL
                     PRQQTSFINLTLEGGRFGAGSPLRETYIRMTLGFTLNDNSWFFKRKFN"
     gene            16793..18196
                     /locus_tag="PK198_04200"
     CDS             16793..18196
                     /locus_tag="PK198_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF37969.1"
                     /translation="MKILRNLILAGLLAGIVNAPLFGQCENWNNSPKKEDAENAHVNY
                     RQYLKGKEVADLEKMEEENFKVAFAEWQKAYTLAPAADGQRASHYQDGRTLYRAMYNK
                     ATDAAKRKEYADVIMRLYDEQIKCYKNEGYLLGRKGYDMFYMEEYGRTQATFDVLKRS
                     LDLSGNNSEYIVFDPISEMVVQKYQAGEMSKDEARNINIRLMEVLDHNIANNARLKTY
                     YETMKARTEITFSAIEDEIFDCAYFKGKLVPKYRENPEDHEVIKYIYNKLRQQGCDEN
                     EEIMVELKAKYGTIASAINDSLRQSYYVDNPAVHAKDLYDSGEYSKAIEKYREAAEKE
                     TDSSKKAEYYLAIASIQFRKLSQYSAARDYALRAARTRSGWGQPYMLIGDMYATTART
                     CGNDGYTRGLAVLAAIDKYAQARNVDSGVADEAGRRIGRISGAKPTGEDVHLRGKAGK
                     TDVVPCWIGETVRLPNL"
     assembly_gap    18412..18434
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18435..19807
                     /locus_tag="PK198_04205"
     CDS             <18435..19807
                     /locus_tag="PK198_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37970.1"
                     /translation="TTAPIVQEEVKPVPATPLQDTTLSPCAKFSDAPNKEDAETNYVL
                     YRDFLKASDWNQAFDYWKKVFAVAPAADGKRNTIFADGVRFYEHFMAQTQDSLQKEQY
                     IDRIFQLYSGIDSCYHEGGYANGRKGFDLFYKYPHRATKEETFQTLLTSAQKDNGMMR
                     DFIINPFTALLVELHEAQKISDADAKKYEQMIRASLAKGLSECKGKDCDRWKIVEEYA
                     PLRLEYFETVKNFYDCAYYKAKYYKEFEAAPNDCDVIRNVYSYLRWAGCADDDTQVVA
                     VVRKGNELCVERERGPAAVAYECLRNADYRCAIDGLNKAAAETNDPVQKSAYLLTVGK
                     IYYAHLKNFPKARQSFQQAADARSNWGEPYIWIGRLYASSGPLCGPGRGWDSQIVVWP
                     AIDMWNKAKRIDPSTASEANKFIGQYEQYMPDRAEVFSRLLKEGDSFFVGCWIQERTT
                     VRAAKQ"
     gene            19937..>20231
                     /locus_tag="PK198_04210"
     CDS             19937..>20231
                     /locus_tag="PK198_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF37971.1"
                     /translation="MFFELSEEHLAVQAAARDFAQNVLKPGVIERDSKMQYPYEEIRQ
                     MGELGFLGMMVSPQYGGGGMDTLSYVLAMEEISKVDNSCSVIMSVNNSLVCWGI"
BASE COUNT         4712 a         5066 c         5063 g         4406 t
ORIGIN      
        1 ctattcgctg gatgattccg ggcagtgaag gcatataaaa aaagcggggc ttttttgcga
       61 aaagccccgc catagccggt gggtattaag tcactcacat gaaacacttc ccacccagaa
      121 tgcttcactg ttttttgtac cgacagcgct ctcagttcat tgtttttctc atggcctcat
      181 agtgtttcaa tagtccgtca ggatgtgttt ttcaataaga ttcacatcac ggataattgn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnata aaaaaagccc ccctcctcgt acaggagaga
      301 ggccatccca aaaaccgatt taaacttgcg ctcagaagcg gtctttgtga attacataca
      361 atccaatcgc catataaaaa actaacaatg agtcggttag cattgcccag aagcgatgtg
      421 cttcggatgc aacgacagca aagcacaccc atgtagggca gttgcaaagc tgaaccaata
      481 aaagcctctc gatgaaatga atgcaggaac cgggggtaaa tactccggct gcaaaaaaag
      541 atttggaatg tagagttgca aaggtaaacc aaaaaagaat aacattttat acttaagctg
      601 taaatttaac ttttttgtac tccctgcgac tttcctccgg ggcaatgttc aacataaaag
      661 cgccctgtgc cgttatgtaa ctttgctttt caagcactca attgtatcaa cacatatttc
      721 atcatcaaaa ccaagaacca tcatgccgat gagaaaattt tccctgttcc tcgttgcctt
      781 gtttacgctt acgttggccc tgtcggcttc cgcacaacaa gacaaaacgc agcgccccag
      841 cccgccgatg acggcaagca gcagctttgg cgacacaaaa ttggtcatca attacggcag
      901 ccctgccgtg aaaggccgcg aaatctgggg caaattggta ccttacggga aggtctggcg
      961 caccggagcc aacgaagcca ccacttttga cgtgagtacc gatgtgatga tcgaaggcaa
     1021 aaagctggct gccggtcgtt atgcgctgtt caccattccc ggcgaagacg agtggaccat
     1081 cattttcaac aaaacagccg atcagtgggg cgcttatggc tacaaggccg aagatgatgc
     1141 actccgcgtg gtcgtcaagc cggcttcgca tggagaaacc gccgaacgct tggtttttca
     1201 tgtcattcct gacggtaata ccaaagctac cgtcaccctg tattgggaaa aagtggccgt
     1261 tgggtttaat atgacgaatg tcaagtaggc gctgattttc tgatattgtc accgcagcga
     1321 aatggccgac atggggagta gaataggttc gttttcagaa tctaagctgc ctgcgtcggc
     1381 tttttcgtgt gcggcgcgca gaaaaaattc ccatctttgc ccctgttttt acaccatcta
     1441 aacggattga aagtgagctt tttcagcaaa ttcttcaaca aggagaaaaa agaggacttg
     1501 gacaagggcc tcgaaaaaac aaaaaccaac ttcctcggca aattggccaa agccgtagca
     1561 ggcaagtcca ccgtggacga ggaagtcctc gacgaagtgg aaaacatcct catctcctcc
     1621 gatgtaggat tggacactac cgtgaaaatc atcgaacgca tagaggcccg tgtggctcgc
     1681 gacaaatacc tgaacgcaag cgaactcagc gaaatcctcc gcgacgagat cgtgcaactc
     1741 ctcagcgaca acaatacgca agacctggag gacttcctgc catccgtgca ggccaagcca
     1801 ttgattttga tggtagtagg tgtgaacggc gtgggcaaaa ccaccaccat cggcaagttg
     1861 gcatggcagt tcaaacaaca gggcaaaaag gtggtcatgg gcgccgccga taccttccgc
     1921 gccgccgccg ttgaccagct taaaatctgg tcggagcgcg tgggctgcga ttttttcagc
     1981 aaaggcatga ataccgaccc cgccgccgta gcgtacgaaa ccgtgcaata cgccatccag
     2041 aacaactgcg atgtggccat cattgacacc gcaggccgcc tgcacaccaa gatcaacctg
     2101 atgaaggagc tgggcaaaat caaaagctcc atcaccaaac gcatggacgg cgctccgcac
     2161 gaggttttgc tcgtactcga tgccaccacc ggtcaaaatg ccattgagca ggccaagcat
     2221 ttcacccagg caacggaggt cactgcgctg gcactcacca agttggacgg cacggccaaa
     2281 ggcggcgtag ccatcggcat ctccgatcag ttcaaaattc ccatcaaata catcggcgta
     2341 ggcgaggcca ttgataaatt gcagattttc aacaaacgcg cctttgtgga ttccctcttt
     2401 gaaaagtaaa cacagcaatc attttgctaa aaagccgttc attcggcgga aagttatatc
     2461 ttcgcaaagt gaatgtggct gcctgagtct ttgtttgcaa atactcgcag cctccgctaa
     2521 aaacaacgct tatgtttggc aacagtaaaa aagataccgg taagccgggc gctcccggca
     2581 ctgcgccctc caattcgctc aacagcctca cgcaaggcgc cgtagtagag ggtaccgtta
     2641 aagccgacag cgacatccgc gtggacggta agataaaagg caaattgcac tgcgccgcca
     2701 aagtcatcat tgggccaagc ggacatattg aaggagaaat ccgttgcaaa aatgccgtca
     2761 tcgagggcaa attcatcggc aatattgacg tagaagaact gctcaatgtg cgcgaaaccg
     2821 cccatataga cggcgaggtc aatgtgggca agctgattgt acaatcgggg gccaccttca
     2881 atgtcacctg caacatgggc ggcgtcaaaa aatcggatca gggcgctgcc ggttccagca
     2941 aagccaatac tgcccatgcc tccgcaggaa aaccacagca agcctgagcc ggaaaagccc
     3001 gatgagcaac gtgcttcccg cgcccgcaac tacatgaagt attcgggcat ggccttccaa
     3061 atgggcggca tcatcctctt cggcgtttgg gtgggcaaaa aattggatga gcgcttttcc
     3121 acctctacac cttggttcac cgtcgggttg gcgctttttt ccgtctttgc tgctttgtat
     3181 attgttctaa aagattttat gaaaacgccc tcatgacgca ccgatccttt ctccttcaat
     3241 tgacattgct tacagccgcc tccgccgcag gattgtggct gatgctgggc atgctgctgc
     3301 tggcgttgct gcacgccccg ctgatgctgg gaatgccgca gttggccggt tttgagtggt
     3361 tcggctgggg ctgtctgata ttttttgtac tgctcaccat cgccatgttt tatgccggtc
     3421 acagtgcggc ccgcagcagc aacaaaaacg attttaccaa tgcggcaatg ggtttttccg
     3481 ggggcaaaat ttttctatcg gccatcatca tcctgatgta tgtagagttg gccgccccga
     3541 cttctaaatt ctttatcctg ccgttttttg cgctgtacat tatatacact gtctttgagg
     3601 tatatttcat gtcgaaactc ggcagaatga agccataagt agtggggcaa aaataaagtg
     3661 ttcattttct ttaggccata atttattttc atcgaatcca tcctgaatca tggaacacgg
     3721 accatacaaa gccatcgtca acggagcgtt tgaatacgaa gacctctctc cggctcaatt
     3781 agatgccgct tcttccggca aaggaaaaca ccatatcctg aacgggccgg tttcgcttca
     3841 tgcagaaatt ttggagacgg attttttcca aaaacgctac gtcatcaagg tcaacggnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctatgga ccagttggtc aaccgtctgg
     3961 ggctttctat taaaacgacg catcgggtca aagacatcaa agcgcccatg cccggcctcg
     4021 tgcgccgcat agaagtatcg cccggccagg aaatcaaaga gggcgacccc atcctgattc
     4081 tggaagccat gaaaatggaa aacgtgctca agtctcccgg cgaagggcgc attgctaaag
     4141 tgctggtttc ggaaggcgcg ccggtagaaa aaggccaggt tttgatagag gtggaatagt
     4201 gctggctacc ttatcaaagt agcatagccc ttgatttcaa aaggctcgcc gnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnacata cacgccgggc ggtgaatctt ggcccgtgtt
     4321 gaattttcgg ccgttccaac cctcattcgg actgtcggtt tcaaaaacca tttcgcccca
     4381 gcgattccaa atactcatat tgaaagcccg cgccccctcc atgatgccca cgcctttgta
     4441 ggtgtcgttc aggccgtcgg cattgggcgt gaaggcattc ggcaggtgaa agcgcacttc
     4501 gggccttata tccactattt gcgtggcggt gtcgcggcag ccgctggggc gtgtcaccac
     4561 ttgtgtcacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnncgtt gccgaaatcc caaaaccagc gattggcgcc ttccgaccgg
     4681 tcggaaaaaa tgacggtatt gttgatgctc gtggggtcat ccggcgtgta ttcaaacccg
     4741 gccacaggcg agggcagcac ccgtatcagg ctgttgaaaa cagtatccgt ttggcagcca
     4801 atgggcgaaa tgatgcgcac ggaaacggtg taaacgcctg tttctgtgta ggtatgagtg
     4861 gggctgatgg ccgtgccggt agcgccgtcg ccaaactccc acaagatgtc ataggtctcg
     4921 tcaatgggaa aagagaggtt gtcaaaaaat atctctgcgg ggcaaccggt aaacgaactg
     4981 ggcgcaatga cgatgagcga aggcaccggg aaataaggca ccactttggt aatgctggcc
     5041 tgacaaccgt tgacatccgt gacggtgagg gtgacgggaa tattgccggg tatgcggtag
     5101 gtatgggcgg ggttgcgggc agaacttctt tggccgtcgc cgaactgcca atcccaggct
     5161 acaatattgc cgctccccga catggataag tctgtaaatt gcacaggccc ggctacgcag
     5221 gtgtcgtagg caaaggagaa atcggcactg atgctgggga atacattgat ggtcactctg
     5281 gcggtgtctc cgcagttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     5341 attgatggtg ggtttccagc tattggaggt gacggtattg cctgaaatat taaacctcca
     5401 taaaaaattg gtcacaaaag cctgctggaa gctcgtattg acaatggtaa cggcggtatc
     5461 tccgcaggca ttgatgaagt aagactgccc gtcggctatg gaatcggact gaatctgggc
     5521 caccaccgta gggtcgcagc gcgccacatt gaactgaaaa tctcgaaata ccgtgctcag
     5581 cagcacgccg ttgcgatact ccagcacctg cacccccacc acatactgcc ccaaattgac
     5641 gggcgttccc gaaatcaggc ccgtttgaga gtcaatggtc actacggggt ttccggccat
     5701 tggggcggtt ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnggcgccga aaaacgatac accaagctgt cgccatctgc
     5881 gtcggtagcg gcatggtcgt aattcaatgg cagccccccg caaatcaccg tagggggaaa
     5941 tgtgttgaag gtcgggctgt tgttgcagag ttgttgggcc gccgaggtga tttcgacagt
     6001 ataggtggcg cccacagttg agggggcaaa gatattattg atggtttcgt tgcggcagca
     6061 gcgctgataa gtgatgtggt agctgtcggt agtggaaaca ggcaaactta aattgaagac
     6121 atactcggcc tcctgcacgc agacattggg cggaatgaga cagggatacg agggcgccgt
     6181 cacctgacga acactcccca agggtacgtc aacccttctg aagggcgcgc cttgcgattg
     6241 gcccgaacag ttgcctccag ggcaacgata cactccaacg aatgctgtgg ggtcgaaatt
     6301 ggcgcaatcc gtgcagttgc aatcccggta cattttgagc gtaaactcat aattgccggg
     6361 gctgatacaa cggtaagtca tcacgccgcc nnnnnnnnnn atgatgtggc gggcatttcc
     6421 tgtggtggtc aggaaaaata atacgaatgc cgcgatggcg aattttccga tagtacgcat
     6481 gtttcgatag ccgggtgtgt gaatgagtaa aatatcgttt gcgctgtcta tgttcatctt
     6541 gctgaacgaa aagaccgcct aacatcttat cgaggtgaag cgactgcccc cgaaagaaaa
     6601 ggattttatc tcagcaaggc tactttttct tctgattcgc cttgtgaatg acagaaaagc
     6661 gactccctgg ctattttttg ggcgcccttt tgctgcgatg tttgagcaac cacggcaaca
     6721 aatccggctc ggcaaaggca ttgtcccagc tattgtggtt cacgccagga tactccgagt
     6781 agcgcacctg accgccggct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnncccat accgcccatc gaaaggccgc ccacatacat
     7021 ccgcgaggca tccgttttgg tgtcgttcat cagcatgtcc accaattcca tgactaagcg
     7081 catgggtttt cccggcgtct caaacaaggg gaacgcccag tctatccgct ccggatcgcg
     7141 ctccactctg gcccaataca ggtctttggg gcattgcggg aacactacaa tggcagggta
     7201 tttggccctg acttcaggtt tcagaaacat tttggcgccg tgtaccaatt gcatttcgtt
     7261 gtcattgccc cgctcgcccg cgccatgcaa gaccaatatc agcgggtacg acttgccggc
     7321 ggcatatcct tcggtaaaaa ggattcggta gggcagcgaa tcgccgtctg cattgatgta
     7381 cagccgacgc tcataagcgc tcaggtcctg cgcgctcagc aggttgaaag agaaaaagaa
     7441 taagaaaaac agtacgatgt atctcatggc agtattattt actcattaag tcaaaacgat
     7501 ccacctcctc cagcgtatcc gggtcgagga cttccacctg aatttttttg cctttcacat
     7561 gaaagaacac cagcgcatgg cgggtggtga acccgctgac gtagggcgtc cagggcgttt
     7621 gttcctgcgc atagtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnngttcg tcgccggtga gaatggcgcg cacctttttg
     7801 cttttattca ccaataaatc cagcatttgg tcgcggcgtt caatgatgcc tttttccatc
     7861 ggctttccgg ctacatacgg gcgcatggca ttgttgccgc ggtaccacat atcgtcgctc
     7921 acatggccgc cattgggaaa aaagggcgtg tgggtggtga cgaaaatatg gtcaacggcc
     7981 ttattgcgct ccaaatctgc aacggttttt tccaaccatt tcaactgcat gtccatgatg
     8041 tacccgtgca tgccgccgct ggtgtggggc agtcgggccg ttgtgggtgc gtaccaatag
     8101 tcagaattga gcactatgac agccacattg tcgtaggtgt agaaatagac gttctctttg
     8161 tacgaaggga agtccgtctg tgaagggttg ggatcgtaag gagcgccgtc ttcgctgtcg
     8221 gggccgttga gcgggttgac gaaattggcg gcaaaaacag cctctgcgga ttcggtttcg
     8281 taaggaaatc tatctatcag aaaagcaatc gaatccgtag gcgttttgtg aacaaagcga
     8341 tgtacgagcg cctcgtggtt gcccatgccc gcgtatatgg gcacgtagtg tgtaaaaggc
     8401 tgcacggcgc gtttccagtt ggcgtattgc aggtgcatgt tgtcggtgtt gttgagatag
     8461 ccgtttacca aatcgccggt aaactgcata aaccgggcct gcttcatttg ccccagcgcc
     8521 atgatttttt tcataatgta atggttggcg ccccatacat tgcgctcgcc gccgccctgc
     8581 cccgcccgcg aatcgctggc ataagtaaag ctaaaagcag tgcggctgcc gggtctcggc
     8641 gcggtgcgaa acgagaagtg ctgccggccg tcgccatagc gcagggtgta tttgtattcc
     8701 gtggcagggg ccagaccgct gagggcaatc tcatgtacgg tacaggctgc cgtgtcgcga
     8761 aaaatgcgcc cgccggcttc cacttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt
     8881 ccacgatggt cggcgccacc tcaaacgggc ctttgcccct gaactccaca atgccatcgt
     8941 aaaacatctg gccggtatcg tcggtcacgc gatagcccac tacgccgcgc tcgtttttct
     9001 gccagcctac catatcatag ctgccagaca taccgatgat gcgaatagca gccttgccgt
     9061 tgacgatgtt gaccggtgcg cgaaagtaaa cggggtgagg gtgccgggca tcgccatgcg
     9121 ggatgaagcc gtagtataat ttgccgttga agcccgcccc gannnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatgttgg
     9301 aatgtgcgcg aaaagcgctg gtgtcgggct gggaatgtac cgtaacggcc cacaaaagca
     9361 ttgcggccag tgcaaaagtg tgtgttcctc tcatggtatg tgcagaaatt gatgagaggc
     9421 caaagataga caaaagcagg tgaatatcct atctcagccc ccgctcctgt agctttttgc
     9481 gatagcggtc cacattgacc aagtgctggg aataggtctc tgcaaaggcg tgcaagcccg
     9541 attcgtcgcc tactgcgcaa aagaaaatgt agttgtgctt ttggtggttc nnnnnnnnnn
     9601 nnnnnnnnnn nnatgcccga aatagaagcc atgccgatgg ggccgggcgg aaggccggca
     9661 tacatataag tattgtaggg cgagtcgaat ttggtgtgat attctgtcac gcgtttggtc
     9721 tcaaaatcgc gggtggcgaa cacgcaggta ggatcggcct gcaatggcat gttgatcttc
     9781 aggcgattga gataggcgcc ggcgatggtg ggcttctccg aacgctgcaa agtctcccgg
     9841 tcaacaatgg aggccagcgt ataaacctcc tccggcgtca tgtcgagtcg gcgggctttt
     9901 tcgaggcggt tctctttgtc ccaaaaagct ttgtgttctt tcaccatgcg ctccgtgaac
     9961 tgctccggcg tgctgttcca atacacctga taggtattgg gaataaacag actcataaag
    10021 gtttcgggcg tgtatccgat ggattgcagg tattcggggt cggaaaacag gacataaatc
    10081 tgcgcggaat ccggctctat gaagcgggct attttggcgg ccacgttttc gggcaggcgt
    10141 tcattggtca gcaccacgtt cacaggggcc tgttcgccgc tgcgcaggtg ccgcaccatt
    10201 tgtatcagcg acatgcccgg cgcgagtttg tacctnnnnn nnnnnnnnnn nnnnnncgtt
    10261 tgtacttcat ttgctcggca atgacgcgaa aagcagccgc atcgcggatg aagcccttgg
    10321 cgagcaatga gtcggtcacc tgatcgaaat tggcgccggt gggcaccagc aggtggtagt
    10381 cgtccagacc gcggggaacg gccgttttgg ccacatatcg ccagtaaaca aaggccgcca
    10441 cgccgcccgc gaggagcagc aacagcaaga gggaacgtaa gagggaacgc tgcatggtgt
    10501 tttagtaggc ttctttttcg ttgggaaatg attgtgattt gacgtcttcg atgtactgct
    10561 cggtagcgcc ccggatgagg tcgtacagtt ccagataacg gcgcagaaaa cgcggctgaa
    10621 aatctttggt gatgccgagc atgtcttgcg tgacgagtac ctgcccgtct gcgctgggtc
    10681 cgccgccgat gccgatggtg ggaatgatga gttcttcaga cactctgcgg gtgagttgag
    10741 cgggtatttt ttccaaaaca atggcaaagc agcccaattc ctgtagcaac ttggcatccg
    10801 acaacagctt ttcggcctca gcttcctctt tggcgcgcac ggtgtaagtg ccgaatttgt
    10861 aaatagattg cggcgtgagg cccaggtgcc ccatcaccgg aacgcccgcc gagaggatac
    10921 gcgctacgga ttcggcaatt tcctccccgc cctccaattt cacggcatga gcgccggctt
    10981 ctttcataat gcggatggtg gatttgagcg ccacagtaga gttgccctga tacgaaccga
    11041 aaggcaggtc caccactacc agagagcgct tgacggcccg caccaccgag gcggcgtggt
    11101 agatcatttg gtcgagtgtg atgggcaggg tggtttcgtg gcccgccatg acattggagg
    11161 cggagtcgcc taccaacagg atgtcggtgc cggcttcgtc gaggatgcgc gccatggagt
    11221 agtcgtaggc ggtgagcatg gcaatttttt cgccgcgcgc cttcatggcc tgcaagacgt
    11281 gggtcgtcac ccttttgact tctttgtgta cggacatggc ctgttccgca tgtttggtga
    11341 agcggcaaag gtagttaaaa cgattgaatg aaatgctcca ggtcttgttc ttcctgcaag
    11401 cccagacgct ttctcaggcg ggtgcgccct ttcttgacgg atgcctcgga gacgcccaga
    11461 atagtagcta tctcgtgacg ggtgaaattg agcttgtaca ataaaaacaa gcgttcctct
    11521 gcattggtaa tatcggaata atgagtgcgc aaacgatgga tgaagcccgg ataagagcgt
    11581 tcaaaagagc gtttgaaggc cgtccagtcc ttctctgtga ggatgtgtat atcgtacagc
    11641 gcaacagatt caggagtgtc cgattcggcg gtatcccctt ctggttcagc ggattcagta
    11701 gggcgctcct gattttgctc taattccatc aggcgggtgt ttttggctaa caacaactga
    11761 gcgaactcct ccaattgctg ccggttttga gccagttctg cctgcaatct gcgggccttt
    11821 tctcttcgga aatacagcca taaaccgatg gccgaaatag taactcccca gagtgccagc
    11881 gccatgttgc gccgcgccac ttcaatttgt tggcgggctt tgagcagttg gttttcccgt
    11941 tctttttctt cggtttcata ttcgattttc aactgctgga tggtgttgtt tttttcttca
    12001 ttgatgacgg agtcgcgcag tattttgaag gttttgtaaa gctgcaacgc ctctgcaata
    12061 ttgccggtgc tttcttcgga catagacaac aattgtaccg ctctggcgtg gatatgcaca
    12121 taccccagac tgtcgcagcc ggcggcggct tccttcagat atttttttgc ggcttcgtaa
    12181 tggcccagat ttctgtgaca gtcgccgatg ttgagcaaca agccaggcag ttggtccaaa
    12241 tacccggaag ctttcatcaa caacaaggcc gaatcgagca agggcagcgc cttagcccag
    12301 tctccggcgc cgctgtaggt gaaggccatt tggttgtaag ctgcacagcg ttcaatcagc
    12361 gagccgtagt tttttaagat ttcaaagcct ttttgtgccg tagccatttg tttttccggt
    12421 atttcggcca aaccgtaaaa cgaagacaat gtagaatacg ccctgccgat gcgggtggaa
    12481 tcgccgctgc gaatgaaggc agccagcgca tcttcaccca gttctatggc tttgggaaac
    12541 tcccggaggc gctcatgcac acaggccaga tcgagttgtg catcgtaata agggaacgag
    12601 tctccttccg cggcggctat ttccatggcc ttttgataaa aagtaatggc ggcggcgtag
    12661 tcaccagcag cagtagcgga atcaccctga tcgttcagtc tttctaaata aggtcggttg
    12721 gcttccatcg catcgtcatt tgtattgccg gagatgcgct cggtagcagg atcgcaggaa
    12781 aaaagagaca agaggcaggc aatgccgaac aggctaaaaa tgaagacttt ggaagtttca
    12841 ctataaattt gttgcatcta tcagcggttt tttggcgcgg tgaagatagg tttcccgtct
    12901 taaaaaactt gatggaatat tgagcgcact caaaaagtgt attacaaata agcaacctga
    12961 gataaaatgg cgcactggaa gtcattccaa aaaacaggag cgagcttcat acccctccct
    13021 atcgccgcat cgcgtttatc gccatctcaa attgcccttt cacttttcat cacagaatcc
    13081 ctacctttgc ggcctctttc atcacgctat tccaagtttc tatgagttcc aagatgatac
    13141 gttccgcttt ggtatccgtt tattacaaag acgggctgga gccgatactc cgcgaattgc
    13201 accgccacgg cgtcaccctg tactctaccg gcggcaccca acagttcatc gaatcgctgg
    13261 gcctttcggc tacggccgtc gaagacatca ccggttatcc atccatcctt gacgggcgcg
    13321 tcaaaaccct gcacccggcg gtattcgggg gcattttggc ccgccgcgaa ccggagcacc
    13381 tcaatcagtt gcagcaatac agcctgcccg aaattgatct ggtcattgtg gacttgtatc
    13441 cctttgagga aaccgtagcc ggcagcgcat ccgagcagga catcattgaa aaaatagaca
    13501 tcggcggcat ttcgctcatt cgggcggcgg ccaaaaactt cggcgacgta gccgttgtgg
    13561 cctctcgaca agagtacggc ctgtttttgg aaatgctcca atccggcaat ggcagcctca
    13621 gccgcgatca acgccgcgaa ctcgcccgcc gcgccttcaa ggtatcgtcc aactacgaca
    13681 tcgccatttt caactacttc aatgaaggta ccaccgacat cgccttcaaa tacagcatcc
    13741 accgctccga catcctccgc tacggcgaga acccgcacca aagcggcgta ttcttcggag
    13801 attttgatca gatttttgac aaaattgccg gcaaggccat ctcctacaac aacttggtgg
    13861 acatagacgc agccgtgcag atcatgcgcg aactacgcaa caccgaaccc gcttttgcca
    13921 tcctcaaaca caccaatacc tgcggcctgg ccctccgcga caccctgccc gaagcctggc
    13981 gcgccgccct ggcctgcgat ccggtttctg ctttcggagg tatttttatc tgcaatcgcc
    14041 cggtggacct tgaaactgcc ggtgaaatcg acaagctgtt ttacgaggtg ctcattgcgc
    14101 ccggtttcga tgaggatgcc ctagcgctgc tacaagccaa aaagaatcgc attttattat
    14161 taatcaaaga cttctatgtg cggccccgga gttttcgcag cttactcaac ggcgtggtgg
    14221 aacaagacac cgacctgcgc caggacacgg aagacgacta tcagaccgct acgctgcggg
    14281 cgccttccgc ctccgaggtg agcgacctgc tctttgccgg cactgccgtg aaacacctca
    14341 aatcgaacgg cattgctttg gtcaaaaaca agcaactcat cggcatggga tgcgggcaga
    14401 cttcgcgcgt ggatgccctg cgacaggcca ttgccaaagc cgccgctttc ggtttcgacc
    14461 tcaaaggcgc cgtcatggcc tccgatgcct ttttcccttt ccccgattgt gtggaaattg
    14521 cacacgaggc cggcattacg gcagtggtgc agcccggcgg ttcggtgaaa gatcaggaca
    14581 gcatagactt ctgcaatgcc cacaacatgg cgatggtgct gacgggagtg cgccatttta
    14641 aacattaaca tcctcttaag ttaccgtctg ttcggcctgc gtcttataaa acaagcgttc
    14701 ataaattttg agcaacttag tcattgacat cggcaacacc cgcaccaaag cggccgtatt
    14761 tcggaacgac gaaatgacgg agcggctgat gtgggaacaa tgcacgcggg aagagatttc
    14821 ccgcgtgctg gccaaccatc gggtagaaaa tgtcatctta tccgtagtgg ggccgccgat
    14881 ggaggaaact ctccggcatt ggctggttgc tcaggttcgt tttttcgagt tgacaccggc
    14941 cctgcacctg ccgttccgaa acttgtacgg aacgcccgcc acactcggta aagaccgggt
    15001 agccgccgtt gcgggggcat tggccatgtt tccgggccgt aactgtttgg tcatagatgc
    15061 cggcacctgc atcacttatg acgtgctgac ggcggagggc gcgtatctcg gaggcaatat
    15121 ctcgccgggt atagccatga gattcaaggc attaccaact ttcaccgccg cattgccatt
    15181 ggtagagcgc ggtccgatgc aagatgccgt gggcagcagt acggaaaccg ccattcgcaa
    15241 cggcgtacaa tatggggtgc tgtgggaaac ggagggcatg atagccgcca tgcagcagcg
    15301 cttgaacgac cttaacgtag tattaaccgg cggagatgcg gatttttttg tgaaaaatct
    15361 gaaaagccgc atctttgcga acgctaactt agtcctggaa gggctgaata aaattttaaa
    15421 ctacaatgtt tgaaaatctt aaaagagttg ttttgggggc tatcctctgt gtgccggcct
    15481 ttgccttgct gaatgcacaa accgccgaca ttgagccgag aagcaactcg ccctattccc
    15541 ggtttggtct tggagacatt gtcaatccgc tttttgcagc ccaaagcggc atgggtggtt
    15601 tgagcgccgc ataccgcgac ccgtttcacc tcaacatggc caatccggcg gcattgggcc
    15661 acctgatggc taccgatttg gaaaccggtt tttttgccaa atacagcagt ctcagcgacg
    15721 gcagcacttc ggcaagtttg tggagcggca acctgaatta ttttgcattg ggtatgccca
    15781 tcatcaatcc catcaaccgg gcattagaga aaaaatcaga caaaataggc ctcggcatgg
    15841 ggtttgtgct gcaaccttac tccaatgtgg ggtacaattt ggtcactacg caggaactcc
    15901 cggaaatagg cgaggctgtt acgagcttca agggcagcgg cggcgcnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnggg cgcttctcgg cgggtgtgaa tctgggctat
    16021 ttgttcggca aaatgtcttt tgaccgcacc gtagagttca gcgatgtagc caaccaagcg
    16081 tacctcacgc gctatttgga cgaacagagc gtatcgggct ttttgtggaa tgccggcgcg
    16141 caggtgtcca ttccattaga aaagaaaccg gaaggagtag gccggtccac caaagaaaaa
    16201 aacctgattc tgggcctgta tggcaactcc tccaccaacc tgaacaccaa caacagccgc
    16261 tttcacaccc ggtacaacca aacttacaat gaccgcgaca ccattctgct ggaaacggat
    16321 tctatcagaa aaggaacatt gcccggcgaa ctggtctttg gaatcaccta cgatcagtcg
    16381 gacaaactca agctgggctt tgaatatgcc ctcggcggct ggagcgcgta tgccaatccg
    16441 ctgcaaggac agacgctcag caatacccgt cgcatttcgg caggtataga gtatgtgccc
    16501 gatatgtttt cgtacaacaa ctactggcag cgcgtacgtt atcgcggcgg catttaccat
    16561 cgcaccgatc cgcgctccgt caacggcaag caactcacag agtacggaat ttccgcaggt
    16621 ttgggatttc ctgtaatttt gccgcgccag caaacttctt ttatcaacct gacattggaa
    16681 ggcgggcgtt tcggcgccgg ctcgccgctg cgggaaacat acataagaat gacgcttggt
    16741 ttcacgttaa acgataattc ttggttcttt aaacgcaaat tcaattaaaa ctatgaaaat
    16801 tctgcgcaat ctgattctgg ctggactgtt ggccggcatt gtcaacgctc cgctcttcgg
    16861 ccaatgcgaa aactggaaca attcgcccaa gaaagaggat gctgaaaatg ctcacgtcaa
    16921 ctatcgccaa tacctcaaag gtaaagaggt agccgatctc gaaaaaatgg aggaggagaa
    16981 ctttaaagtg gctttcgccg aatggcaaaa ggcttacaca ctggcccccg ccgccgatgg
    17041 tcaacgcgcc agccattatc aagatggacg caccctctat cgcgctatgt acaacaaggc
    17101 caccgatgcg gctaaacgca aagaatacgc cgacgtcatc atgcgcctgt acgacgagca
    17161 gatcaaatgc tacaaaaacg agggctattt gctgggccgc aaaggctacg atatgtttta
    17221 catggaagag tatggtcgca cccaggccac tttcgacgtg ctgaaaaggt cgttggattt
    17281 gagcggcaac aactcagaat acatcgtttt tgaccccatc tccgaaatgg tggtgcaaaa
    17341 atatcaggcc ggcgaaatga gcaaggatga agcccggaac atcaacatca gactgatgga
    17401 agtactggac cacaacattg ccaacaatgc ccgcctcaaa acttattacg aaacaatgaa
    17461 agcccgcacg gagataacct tcagcgccat agaagacgaa attttcgatt gcgcttactt
    17521 caagggcaag ctcgtgccga aataccgcga gaacccggaa gaccacgagg tcatcaaata
    17581 catctacaac aagctccgtc agcagggttg tgatgaaaac gaggaaatca tggtggaact
    17641 gaaggccaaa tacggcacca tcgcttccgc catcaacgac agtctccgcc agtcttatta
    17701 tgttgacaac ccggccgtac acgccaaaga cctctacgac agcggcgagt attctaaagc
    17761 catcgaaaaa taccgcgaag ctgccgaaaa ggaaaccgac agcagcaaaa aagcagagta
    17821 ctatctggcc attgcttcca tacagttccg caaactttca cagtattcgg ccgcccgcga
    17881 ctacgcactg cgcgcagccc gcacacgcag cggttgggga cagccctata tgctcatcgg
    17941 cgatatgtat gctaccacag cccgcacttg cggcaacgat ggctacaccc gcggattggc
    18001 cgttttggcg gccattgaca aatacgctca ggcccgcaac gtggactccg gcgtagccga
    18061 cgaagccggc cgtcgcatag gtcgtatcag cggcgccaag cccaccggcg aagatgttca
    18121 ccttcgcggg aaagccggca aaacggatgt tgtaccctgc tggataggcg aaaccgtgcg
    18181 cctcccgaat ttgtaaaaga accacacagc cctgcccgat agagcaggac agaagagggg
    18241 caagaggccg gccagacgat aacgggtttt tcccggctcg tatgaactgc ttcttgccct
    18301 ttctcatttt taaaccaact gttttttctc taatcttccc aaaacacaaa agccatgcgt
    18361 accaaaaatt ttttgtcctt tgccgcagcc gttatcctgg cgtctgtcat tnnnnnnnnn
    18421 nnnnnnnnnn nnnnaaacga cggccccgat agtacaggaa gaggtaaagc ctgtgccggc
    18481 cactcctttg caggatacca cgcttagtcc ctgcgctaaa ttttctgatg cccccaataa
    18541 ggaagatgca gaaaccaatt atgtgttgta tagagacttt ctcaaagcca gcgactggaa
    18601 tcaggccttc gattattgga aaaaggtgtt tgccgtagcc cctgctgccg atgggaaacg
    18661 caataccata tttgccgatg gcgtgcgctt ttacgaacac ttcatggctc aaacacagga
    18721 ttcgctccaa aaagagcagt atatagaccg catatttcaa ctgtacagcg gcattgacag
    18781 ttgctaccac gaaggaggct acgccaacgg gcgaaagggt tttgatttat tttacaaata
    18841 cccgcatcgc gctaccaaag aagaaacttt tcaaacgctg ctgacttcgg cccaaaagga
    18901 caacggcatg atgcgggact tcatcatcaa ccccttcact gcattgctgg tagaactgca
    18961 cgaggcacaa aaaataagcg atgccgacgc caaaaaatac gagcagatga tccgcgcttc
    19021 attagccaaa ggattgtctg aatgtaaagg caaagattgc gaccgatgga agattgtgga
    19081 agagtacgcc cccctgcgat tggaatattt tgaaactgtg aaaaactttt acgactgcgc
    19141 ctactacaag gccaagtatt ataaggaatt tgaggctgcg cccaatgact gcgatgtgat
    19201 ccgcaatgtg tacagctacc tgcgctgggc cgggtgtgcc gacgatgaca cacaggtagt
    19261 agcagtagtg cgcaaaggca acgaattgtg cgtggagcgc gaaagaggcc ccgcagcagt
    19321 tgcctatgaa tgcctgcgca acgccgatta tcgctgtgcc attgacggcc tgaacaaagc
    19381 cgctgccgaa accaatgacc cggtgcagaa aagcgcctat ttgctcactg tgggcaaaat
    19441 ttactatgcg cacctcaaaa acttcccgaa ggctcgccaa tctttccaac aggccgccga
    19501 tgcacgctcc aactggggag aaccctacat ctggatcggg cggctctatg cctccagcgg
    19561 cccattgtgc ggcccgggcc ggggctggga cagccagata gtggtgtggc cagccataga
    19621 tatgtggaac aaggccaagc gcattgaccc gtccaccgca tcagaagcca acaaattcat
    19681 cggccagtat gagcagtaca tgcccgaccg cgctgaagtt ttctcccgcc tgctcaaaga
    19741 gggagactct ttcttcgtag gctgctggat acaggagcgc actacggtga gggcggctaa
    19801 gcagtagttt ttaatattct atcctgccgg cagaggttgg gcgacggctc tggagggagc
    19861 gcgctcaacc tctgtttttt tatggcatca ttttcccttt ttccgtacct ttgcccgcgc
    19921 caaaaaagaa ccaaatatgt tttttgaact atccgaagaa cacttggccg tacaggccgc
    19981 cgcacgcgac tttgcccaaa acgtcctcaa gccgggcgtc attgagcgcg acagcaaaat
    20041 gcagtatcct tatgaggaaa tccgccagat gggcgaactc ggctttctcg gtatgatggt
    20101 gtctccccaa tacggcggcg gtggcatgga tacgctttcc tacgtccttg caatggaaga
    20161 aatttccaaa gtggacaatt cctgctccgt catcatgtcg gtcaacaact ccttagtctg
    20221 ttggggcatt g
//
LOCUS       DAORLF010000028        20009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1452_length_20009_cov_1.970733, whole genome shotgun sequence.
ACCESSION   DAORLF010000028 DAORLF010000000
VERSION     DAORLF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_20009_cov_1.970733"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK198_04215"
     CDS             complement(<1..554)
                     /locus_tag="PK198_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRF37972.1"
                     /translation="MNTLDNPIEYLKGVGPLRGDMLKKELQIFTQGDLLFQFPYRYVD
                     KTQFHRIADLDDNSGYVQLRGILRSLDTLGEGRKKRMTGRFRDESGVIELVWFTGIQW
                     LQNHLVVGTEYVVYGRVNAFRDQLNIPHPEMEAVGADQSQPAVSFDPVYASTEKLNNK
                     GLDVKARRRLVKAVLEKISERDLP"
     gene            complement(603..>1509)
                     /locus_tag="PK198_04220"
     CDS             complement(603..>1509)
                     /locus_tag="PK198_04220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF37973.1"
                     /translation="PLYFQPFWLDAVAGSDGWDAALAFDRGGHVTGLWVYCLSRYYGF
                     QVVKMPPLTAYSGPLLFYPANMTAQESRYAFDKKVLGELIEQLPPTAFFYQEWHPDLR
                     NWLPAHWKGFRQTTRYTYLLPDLQDWNAVFDGFRSNVRTHIRKAEDLHVFESDDVEAV
                     FRLYALSLEKQGLSAPATLPVLQRIDEVLHARHQRLLLLAEDEQGRRHAGIYVVWDEQ
                     SAYYLLSGADPGLRSSGALYRLVWEALQWAGRKRLSFDFEGSMLEPVEEVFRGFGAQL
                     TPHHKVFRPGNRLFGLLWEWKGWGR"
     assembly_gap    1510..1540
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1566..1745)
                     /locus_tag="PK198_04225"
     CDS             complement(1566..1745)
                     /locus_tag="PK198_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37974.1"
                     /translation="MEPLQFYLTVCLMLGLYWMARHPAWLAGCTDTLLTDPISGTILA
                     TLRRPCFVEPNIPTQ"
     gene            1932..3455
                     /locus_tag="PK198_04230"
     CDS             1932..3455
                     /locus_tag="PK198_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRF37975.1"
                     /translation="MGEHKVRHVQHFEDRARFMPRLIEDVATLEAMLQESAFEQKIQR
                     IGAEQELALLNPDCDPAFLGPQILETMTDPHFTTEIGRFNLEINLDPFPLSHDCLHRM
                     EQQLAEMLRTGNAAAHALDARILLCGIMPTLTYRHLGRDAMTPNPRYYALSDAIHAMR
                     GRDFEVFLTGVDDMITAMDSLVFESCNTSFQTHLQIPTEEFTERYNWAQMISGPMLAI
                     CANSPLLFGRELWSETRIALFQQAVETRTSANQLRNQQPRVFFGNRWIQHSIAELFKD
                     NIARFPVILTKEVPDSSMAQFLSGQAPSLAALRLHNSTVYNWNRPCYGILDGQAHLRI
                     ECRYIPSGPTALDEMANFALWLGLMQGMPEKYKDFHRRVSFRSAKDNFMRAARIGINA
                     MMDWFGEAVSPARLFEKELLPAAREGLSRSGVNDQDADRLLGVVAQRIHKCQTGARWQ
                     IRNFRKLQDHFGSAVALTELTKMMYEHQQTDNLCMNGPTWSGGVPIFLIASAARSVK"
     gene            3452..>3742
                     /locus_tag="PK198_04235"
     CDS             3452..>3742
                     /locus_tag="PK198_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF37976.1"
                     /translation="MMITDVYTVQEEEPLCLVKSIMEWKNIRHLPVENAEGDLTGLVT
                     ATNLSEFENSGDGWEQQPIKNFMVCELLTVAEDAPLDAMKDLMIQHGVGSVLV"
     assembly_gap    3743..3813
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3890..4720)
                     /locus_tag="PK198_04240"
     CDS             complement(3890..4720)
                     /locus_tag="PK198_04240"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRF37977.1"
                     /translation="MAEYYQELDRGFHLYWMEVLRDYGPETVHGLRVNLKQQRAFFYM
                     LEALAPSFSAGEAMDVFEGVYRKAGKVRDRQVERALLEKNERRLHIEHRFSAWLEEQE
                     GRRALLLQQYESGHSMLPVRKLSEQVFQIIRELPEQGAEARLQAYYVKIIRGVIAFIR
                     GDKAAGDDLHDLRKFIKELFYNLRFLQRHTGPEALQCGALARLDDLQRALGKWHDFDF
                     TVVHLQEKKKALNAGLLAHLEAERQASKAEARAMFEGLEAALEELERRVERQLGVSAA
                     "
     gene            complement(4735..>6342)
                     /locus_tag="PK198_04245"
     CDS             complement(4735..>6342)
                     /locus_tag="PK198_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF37978.1"
                     /translation="LNLTVRAPIATTLNRAICQGQSFSVGSSVYTTAGSFTDVLTSVT
                     GCDSTVTLNLVVNPVYEITLTERICDDQTFRVGNMNFSATGTYQVRLTSVAGCDSIVN
                     LILTAHPCTLQFSSSSGAANCFGASTGSVQFSMTVGTPPYSYQWQAVGGALNGMGNVA
                     TNNSSVNIPNLPAGSYRIVVTDFYGIVQTINVAVTQPTPVTVSLAGSNYNGFNVSCPG
                     ADDGEVTVTVAGGNAPYSYQWSTGSAQATLRNVPAGQYQLTLTDANGCTSTPAVPSIT
                     LNGPPPIVVSATATDPPCFGQVQGSISIGEVSGGVGPYLYALDNDPFSSRSLFGGLPV
                     GPYIVRVQDANGCTQQTSVTVNQPQELIVDLGTDLSIKLGEDVQLEALASYPVEMFSW
                     QGDSLSCYDCFNPMARPLSTTTYSVTVTDRNGCKDSDIITVFVDKARDVYFPTAFSPN
                     DDGVNDRFYPFSGSNVAMVKSFYVFNRWGESMFELFNFQSNNPSYGWDGTHRAQRLNT
                     GVYVYVAEVEFIDGEVVIFKGDVTLIR"
     assembly_gap    6343..6440
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6441..>6835)
                     /locus_tag="PK198_04250"
     CDS             complement(<6441..>6835)
                     /locus_tag="PK198_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37979.1"
                     /translation="GTFTDVLTSVTGCDSTVTLNLTVHPIPMTNLVRTICAGESFTVG
                     SSTFNTSGTFQTILTAATGCDSIVNLNLTVRAPIATTLTRAICNGESFTVGTSSYTTS
                     GTFSNVLTSVTGCDSTVTLNLTVHPIPMT"
     assembly_gap    6836..6984
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6985..>7907)
                     /locus_tag="PK198_04255"
     CDS             complement(<6985..>7907)
                     /locus_tag="PK198_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37980.1"
                     /translation="LLTASNGCDSTVTLNLTVLPTPQVTLTEAICAGETFSVGSSNYT
                     TSGVYTNVLTATNGCDSTVTLNLTVHPIPVTNIVTSVCTGASYTVGTSVYTVGGSYRD
                     TLVAVTGCDSIVNLTLTITSFYETNLTRSICEGESVTIGSSTYTISGSYQDVFIAQDG
                     CDSLVNLALTVFPIPVTNLVQVICQGESFSVGSSTYTTSGMYQDVLTAVTGCDSIVNL
                     NLTVNPTFTTTLTEAICDGESFTVGTSTYTTSGTYTDVLTASNGCDSTVTLNLTVHPI
                     PMTNLVRTICAGESFTVGSSAFNTSGTFQTIL"
     assembly_gap    7908..8018
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8019..10561)
                     /locus_tag="PK198_04260"
     CDS             complement(<8019..10561)
                     /locus_tag="PK198_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37981.1"
                     /translation="MQWIGFVAGSSNITMSITPVNCNLGNNNGLQVGIWGTTDCSSFS
                     LVSNCVYQAPPNQATTLTMTGLTVGGTYFFVVDGHSNDVCQFTVNVTSGSTVAPPVTA
                     APTIAAPPTPYCPGAMYTFSTPGVPNAGVYNWTVNGSPAGSSQTAEITFPTNGTYTIC
                     VTPSNVCHGDGPQTCTTVNVGNLALITDPAVNICEGESYSAGGMVFTSPGTYDYNDVS
                     PQGCVQPRRFTLNVRPHKVTNLPTYEFCSNVPQLVTVGGQIFSAPGSYSVPLQTSFGC
                     DSIVNFQINVNNPDLTLLNEEICSPAGFTIGNQTFFSSSPPGNPHMVTLPNQYGCDSL
                     VVLNLTVYSPQNITVRDTICQGQFYQLGNVFYNATGVYQQNITGPGGCTNNTTLYLTV
                     SNPMRTLRDTICNGQSVTVGGTSYNTTGTYTRVLPGASSLGCDSTVILHLTVRAAITR
                     TLNQSVCAGDGFSVGSNTYFNAGTYVNVLTASNGCDSTVTLNLTVLPEPVTNLNVSIC
                     AEDTYTFGGTNYNTSGAYQRVFSAANGCDSTVNLNLTVRPAITTTLNRSICDGESFSV
                     GGNSYSTAGTYTNVLTAANGCDSTVTLNLNILNVPVTNLNITRCFGQSYTVGTNTYTA
                     TGMYSDTLTAVNGCDSIINLNLTIRDRIETTLNVEICTGQTYTLGSNTYDAEGSYQAV
                     FTSVATGCDSTVYLNLSLTDLLTSSLDVSICNGESYTVGTNTYTATGSYDNFFVTPAG
                     CDSLLTLNLTVLPTPQTTLTQNICNGESFTVGTSTYSATGIYTDVLTAANGCDSTVTL
                     NLTVLPTPQTTLTISICDNETYSVGTSTYNLSGTYTDVLTASNGCDSTVT"
     gene            10596..>10835
                     /locus_tag="PK198_04265"
     CDS             10596..>10835
                     /locus_tag="PK198_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37982.1"
                     /translation="MGGTCWPAVVVLFELEKVVKSHSTQESAQVLDSAMVLGGHKSGN
                     CACEMVAVANSKIVASRILIIFNRLSFLMKQKWLLW"
     assembly_gap    10836..10864
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11068..>11261)
                     /locus_tag="PK198_04270"
     CDS             complement(11068..>11261)
                     /locus_tag="PK198_04270"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRF37983.1"
                     /translation="ETGGFSINPGSVMGSIKGAFTTDQHTGTLIPVFAYGPGAELFGG
                     VYENTAIYHKMRRALGFDK"
     assembly_gap    11262..11355
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11356..12193)
                     /locus_tag="PK198_04275"
     CDS             complement(<11356..12193)
                     /locus_tag="PK198_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRF37984.1"
                     /translation="MRIYRLFVFALFSLAACSAPKPASETAPSGAGSIELQKRPQNII
                     LLIGDGMGLGQISAGLYANGNQLNLERCTVTGLHKSYASNDLITDSAAGATAFACGVK
                     TYNGAIGVDAAGKPAKTILEEAQQRGLATGLIATSSITHATPASFIAHVANRGEEEAI
                     ATFFPDSGIDLFIGGGKKFFDRRETDERDLLQQLRKKGYVISHYAEKDLAQITPDPAH
                     PFGYLSADNSPLTAESGRDYLEPAARMAPPFLAKRSNKGFFLMIEGSQIDWGGHAKDA
                     QYI"
     assembly_gap    12292..12327
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12328..>12670)
                     /locus_tag="PK198_04280"
     CDS             complement(<12328..>12670)
                     /locus_tag="PK198_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRF37985.1"
                     /translation="LSDAVVVVESGISGGSIITAKIANGYNKDVFALPGRLHDKMSLG
                     CNHLIKTNQANLLESAADLGYIMQWEERSKSRNIQPQLFVELSDQEQIIVNLLRERSE
                     LSIDALTFEAGI"
     assembly_gap    12671..12741
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12742..>13078)
                     /locus_tag="PK198_04285"
     CDS             complement(<12742..>13078)
                     /locus_tag="PK198_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRF37986.1"
                     /translation="PARLKPFRDSPVMLYYRGNADLNAARTVGIVGTRTPTHYGTAFC
                     EELVDGLKAYDATIISGLAFGIDVTAHRRCLENGMPTIAALGHGLDRIYPPQHRHTAL
                     RMTENGGLLA"
     assembly_gap    13079..13109
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13110..13415)
                     /locus_tag="PK198_04290"
     CDS             complement(<13110..13415)
                     /locus_tag="PK198_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF37987.1"
                     /translation="MTGTKSPRSFAQNHAKSSEQYLHGKDPELLYKVAITQIPMVGAV
                     TARTLISYCGGVEAVFRAKKKELCKIPGVGDLVADYVINQDVLGHAEQELEFMEQHGI
                     "
     gene            13467..13979
                     /locus_tag="PK198_04295"
     CDS             13467..13979
                     /locus_tag="PK198_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF37988.1"
                     /translation="MSTGRKNSRLELVVISVFFILFSFWALSRCNNKRAEMRRQMEAE
                     AMDERRQDSIDRAAAMARMLPPKDTTPAASATPAAPQVIKEQVTPLYVVLDELNMRGE
                     PKLDGRLVARLKLYDELVYLNEVTEFKQEVNLGGVTYNEPWIKVKNKRGQVGWVYGAG
                     VHYYRFRRES"
     assembly_gap    14262..14279
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14280..14812
                     /locus_tag="PK198_04300"
     CDS             <14280..14812
                     /locus_tag="PK198_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37989.1"
                     /translation="FWWGFPFILVGLILLAGYFLLGTINSTALLLQQGKIDEAEKRLA
                     LTFWPQLLYVANRSYYYMIKGNIALSKKDTEAGEMWLRKAQEVKLPSDNEKAMLELQL
                     ANIAASKGKWSQAQLHFRNLKDLKITETAIKDQVKQFEKALESRGQMKAAMRMGNPGA
                     AGMMQPGGKRRRPKMR"
     gene            15133..16530
                     /locus_tag="PK198_04305"
     CDS             15133..16530
                     /locus_tag="PK198_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF37990.1"
                     /translation="MLYFHPMKVRFSIVLALLITVGYILTAQDFRLAYIEQYKSIAIQ
                     EMERAGVPASIKLAQAILESDFGRSDLARRANNHFGIKCGVNWDGREFYKEDDDYDDN
                     GKLVKSCFRVYRSPEASFIAHSEFLRDPAKDFRYGFLFRLDPTDYRSWANGLKRAGYA
                     TAANYHERLINLIETYKLHEYDRLGQAPVVDVTVPNQPGAPAFGYNNDVRFVLSANNE
                     TVSTISRRTDVSLGQLIKYNEQLNDGEQRLAEGTMVYLQPKRNAFRGRAESHLVKEGE
                     TMFDISQTYGIKLAKLLQRNRMEKGMEPAVGQNIKLRGGKVKQRPALRGEATLDKPVN
                     GVRIEMEEAPAPPPAAPTPPPTVQPERESGIRPVTPAPTPTTPANTQPADTPPLAPVK
                     PEEPRPSTPVVQPAPSTPVTTPPPATAPVYHTVEKGDTLWNISQRYQTTVAAIRQLNN
                     MTNDSIQLGQRLRVK"
     gene            16608..>17283
                     /locus_tag="PK198_04310"
     CDS             16608..>17283
                     /locus_tag="PK198_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37991.1"
                     /translation="MKKLLVFVAIIMAAQGLWAQGYVFGIKGGPTFGLQNWGNSFQRD
                     PLIRYHGDVFIESLPESNQWSLFAQAGYHARGSAIRNRNFINPLNGNFFRPPADVFLF
                     HNAAIVLGAKQKFDLGLSSKWFYTFGVRGEYTIDTNLDRYDAFLELNPSIAAVYPINA
                     YFEDNGLLGIRRINYGFSGGAGLEWQFSEFLGASLEFTINPDFSNQYTQPAIPNVRDP
                     WTGELRS"
     assembly_gap    17284..17329
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17400..18140
                     /gene="lptB"
                     /locus_tag="PK198_04315"
     CDS             17400..18140
                     /gene="lptB"
                     /locus_tag="PK198_04315"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRF37992.1"
                     /translation="MILRCENLKKLYGRRQVVHDVSLHVNKGEIVGLLGPNGAGKTTT
                     FYMTVGFIKPNEGRVFLDNQDITDMPMYRRAQHGIGYLPQEASVFRKLSVEDNVRAVL
                     EMTHLNKSQQKEKLESLIEEFGLEKVRRSRGDTLSGGERRRTEIARALATDPSFILLD
                     EPFAGIDPIAVEDIQYIVAKLKTKNIGILITDHNVQETLSITDRAYLMFEGGILRAGT
                     AEELAEDEMVRKVYLGQHFELRRKVIHV"
     gene            18140..18409
                     /locus_tag="PK198_04320"
     CDS             18140..18409
                     /locus_tag="PK198_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37993.1"
                     /translation="MRSRPYLLPIFVCAALLYGGCTDDAPTRLSSDQLQLLDSLVTVQ
                     MPLVRQEEDSICKADFDKKLRRMVDSMLVIRREEEARLRQRMPKQ"
     gene            18406..>18648
                     /locus_tag="PK198_04325"
     CDS             18406..>18648
                     /locus_tag="PK198_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37994.1"
                     /translation="MSRYSYIFLLFAALSVVAVSCDDSAKQKAQEEALLQSRLLERLE
                     MGRVTRREECMRQLADSALVIVDSIRLEDARWALDTL"
     assembly_gap    18649..18687
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18802..19875
                     /locus_tag="PK198_04330"
     CDS             18802..19875
                     /locus_tag="PK198_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRF37995.1"
                     /translation="MIVLKLIVGLILLVVGAEALVRGASRIAASFGITPLVIGLTVVA
                     FGTSSPELAVSVMSSLSGASDIALGNVVGSNIFNVLFILGISALIIPLAVHQQIIKMD
                     VPILIGLSLLTWWMASDGNIQVWEGSILILLGIAYMLLLIRMSKKEKKAIEQEYDEAF
                     GEQKGKSGKIWMSALFVLAGLALLVLGSRWLVDGAVYIAKSLGVSDLVIGLTIIAAGT
                     SLPEVATSIIAALRGERDIAVGNVVGSNLFNIFFILGIAAVSGGGIGVADSAIRFDLP
                     VMTVVAFACLPIFFTGSKLDRWEGAVFLLYYIAYTAYLILASQNSSSLEAYSHAILWF
                     AVPITILTLGIVTWRQAKRNKAV"
BASE COUNT         4413 a         5116 c         5263 g         4393 t
ORIGIN      
        1 tcgggcaggt cgcgctcgct gattttctcc aaaacggctt tcaccaaacg gcggcgtgcc
       61 tttacgtcca agcctttgtt gttgagcttt tcggtggagg catacacggg gtcgaaactg
      121 acggcgggct gactctgatc ggcgccgacg gcctccatct ccggatgcgg gatgttgagc
      181 tgatcccgga acgcattcac gcggccatac acgacgtatt ccgtaccgac gaccaagtga
      241 ttttgcaacc attgaatgcc ggtgaaccac accaattcga tgacgcccga ttcatcgcga
      301 aagcggccgg tcatgcgttt tttgcggccc tcgcccaggg tgtccaagct gcggagtatg
      361 cctcggagtt gcacatatcc gctgttgtcg tcaaggtcgg cgatgcggtg aaactgggtt
      421 ttgtccacat accggtaggg gaattggaaa agcaggtcgc cctgggtgaa aatctgaagt
      481 tcttttttca gcatatctcc ccgcagcggg cctacgcctt tgaggtattc tatcgggttg
      541 tccaacgtgt tcattatcag tagaaatggc tttgtggcgg ggtaaagata ggaatttttt
      601 cctcaccgcc cccagccctt ccactcccac agcagcccaa acagccggtt gccgggccgg
      661 aacaccttgt ggtgcggcgt cagttgcgcc ccgaagcccc ggaaaacctc ctccacgggt
      721 tcgagcatgg agccttcaaa atcgaaggag aggcgcttgc ggcctgccca ttgcagcgcc
      781 tcccacacca agcggtacag tgcgccgctg ctgcgcaggc cgggatcggc gccgctgagg
      841 agatagtacg cactttgctc gtcccatacc acatagatac cggcgtggcg gcggccctgc
      901 tcgtcttcgg ccagcaggag gaggcgttga tggcgggcgt gcagcacctc gtctatgcgt
      961 tgcagtaccg gcagcgtggc gggggcggaa agtccttgtt tttcaagcga taaggcgtat
     1021 aatcgaaaga cggcttccac atcgtccgat tcaaaaacgt ggaggtcttc ggctttgcgg
     1081 atgtgggtgc gtacattgct gcggaagccg tcgaacacgg cgttccagtc ctgcaaatcg
     1141 ggcagcaggt aggtgtagcg ggtggtttgg cgaaagcctt tccagtgtgc cggcagccag
     1201 ttgcgcagat cggggtgcca ttcctgataa aaaaaagcag tgggcggcag ttgttcgatg
     1261 agttcgccga gtactttttt gtcaaaagca tagcggcttt cctgcgccgt catgttggcc
     1321 ggataaaaca gcagcggccc ggagtaggcc gtaaggggcg gcattttgac tacctgaaag
     1381 ccataatacc ttgacagaca atacacccac agcccggtaa cgtgccctcc gcggtcgaag
     1441 gctaaggctg catcccagcc gtcggagccg gcgactgcgt cgagccagaa aggctggaaa
     1501 tagagcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtagaggg attgggaatt
     1561 gctgctcatt gagttggtat gtttggttcg acaaagcagg gtcttcgcaa agtggccagg
     1621 atcgttcccg atataggatc ggtgagcaag gtgtcggtgc agcccgccag ccatgccgga
     1681 tgccgggcca tccagtacag tcccagcatg aggcaaacgg tgagataaaa ttgcagcggt
     1741 tccatgcagc gctcgttttc tttctgcaag atgcgaactt ttggcttatg gaagtcaaat
     1801 tttgccacac aagccgaagt ggccgcttca ttttcgtttc cacaaaaatc acctcctttg
     1861 ttgatatacc tcattgacat acaagcccaa aggcctgcaa tttgaggctt tcacccaaaa
     1921 ccggttttgt catgggagag cacaaagtca ggcatgttca acattttgaa gacagggcgc
     1981 gattcatgcc ccgcctcatt gaagacgtgg ccacgctgga ggccatgctg caggaatcag
     2041 catttgaaca gaaaatacaa cgcatcggcg ccgagcagga actggcgttg ctcaaccccg
     2101 attgcgaccc cgcttttctg gggccgcaaa tattggagac gatgaccgac ccgcatttca
     2161 ccaccgaaat cgggcgcttc aatctcgaaa tcaatctcga tccctttccg ctgagccacg
     2221 attgtctgca ccgcatggag caacaactcg ccgaaatgct gcgcaccggc aatgcggctg
     2281 cacacgccct cgatgcacgc atcctcctct gcggcatcat gcccacgctc acctacaggc
     2341 acctcggccg cgacgccatg acgcccaatc cccgctacta cgccctcagc gatgccatac
     2401 acgccatgcg gggccgcgat ttcgaggtgt ttctcaccgg tgtggacgac atgattacgg
     2461 cgatggacag cctcgtgttc gagtcttgca acaccagttt tcaaacgcat ttgcaaattc
     2521 ccacggagga gtttaccgag cgctacaact gggcgcaaat gatctccggg ccgatgctgg
     2581 ccatctgcgc caactcgccg ctgctcttcg ggcgagaact ctggagtgaa acccgcattg
     2641 ccctgtttca gcaggccgtc gaaacccgca ccagcgccaa ccaactccgc aaccaacagc
     2701 cgcgcgtctt cttcggcaat cgctggattc agcactccat cgccgagctt tttaaagaca
     2761 acatcgcccg cttcccggtc atcctcacca aagaggtacc cgacagcagc atggcccagt
     2821 ttctcagcgg acaggcgccc tcgctggcag ccctccgact tcacaacagc accgtttaca
     2881 actggaaccg cccctgctac ggcatcctcg acggccaggc acacctccgc atcgagtgcc
     2941 gctacatccc ctccggcccc accgccctcg acgaaatggc caactttgcc ctctggctgg
     3001 gcctcatgca gggcatgccc gaaaagtata aagacttcca ccggcgggtg tcgttccgct
     3061 ccgccaaaga caacttcatg cgcgctgccc gcatcggcat caacgccatg atggattggt
     3121 ttggtgaggc tgtttcgccc gcccggctgt ttgaaaaaga actgttgccg gcggctcgcg
     3181 aggggctttc cagatcgggc gtaaatgacc aggatgcaga ccgcctgctg ggcgtcgtgg
     3241 cgcaacgtat tcacaaatgc caaaccggcg cgaggtggca aatcaggaat ttcagaaaat
     3301 tgcaggacca tttcggatca gccgtcgcgc tcaccgaact gaccaaaatg atgtacgagc
     3361 atcagcaaac cgacaacctg tgcatgaatg gcccgacatg gagtggggga gtacctatat
     3421 ttttgattgc gagcgctgcc cggtcggtaa aatgatgatt acggatgtct atactgtgca
     3481 ggaagaagag ccgctttgcc tcgtcaaatc tattatggag tggaaaaaca ttcgccacct
     3541 gccggtagaa aatgccgagg gcgatctcac gggcctggtg acggccacca atttgagcga
     3601 atttgaaaac tccggcgatg gctgggaaca acagcccatc aaaaatttca tggtgtgcga
     3661 actgctcacg gtagccgaag acgctccgct ggatgccatg aaagatttga tgatacaaca
     3721 cggggtgggc agtgtgttgg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaagaag cgctaattgt agtatgtaca
     3841 tagtgaagac ctgaatccgg ggcggtggtc ttggcctgtc atccctttac tacgctgcgg
     3901 acacccccaa ctgcctttct accctccgct ccaactcctc caaagctgcc tccaagccct
     3961 caaacatggc gcgggcctcc gccttcgaag cctgtcgctc tgcttccaaa tgagcgagaa
     4021 gaccggcatt cagggctttt ttcttttctt gcaaatgaac caccgtaaag tcgaaatcat
     4081 gccacttgcc gagggcacgc tgtaagtcgt ctaaacgtgc caaagccccg cactgcaagg
     4141 cttccgggcc ggtatgccgc tgtaggaaac ggaggttgta aaagagttct ttgatgaatt
     4201 tgcgcaaatc gtgcaggtcg tcgccggcag ctttgtcgcc gcgtataaag gcaatgacgc
     4261 cccgaatgat ttttacataa tacgcctgca aacgcgcttc tgctccttgc tccggcagtt
     4321 ccctgatgat ttggaaaacc tgctccgaca atttcctcac cggcaacatg gaatgcccgc
     4381 tttcgtattg ctgcaacagc aaggcccgac gtccttcctg ttcttccagc caggccgaaa
     4441 accggtgctc tatgtgcagc ctgcgttcgt ttttttccaa cagggcgcgt tccacttgcc
     4501 ggtcgcgcac tttgccggct ttgcgataca cgccttcaaa aacatccatt gcttcgccgg
     4561 cagaaaatga aggagccagg gcttccaaca tgtagaagaa agcgcgctgc tgcttgagat
     4621 tgacccgcag gccatgcacc gtttccggtc cgtaatcgcg cagcacctcc atccagtaca
     4681 ggtggaagcc ccggtccaat tcctgataat attccgccaa tagccgaacg atgctcatcg
     4741 aatcaaggtc acatctcctt tgaagataac aacctctcca tcaataaatt ccacctcggc
     4801 tacatacaca tacaccccgg tattgagcct ttgggcgcgg tgcgtgccgt cccaaccgta
     4861 agaggggttg ttggactgga aattgaagag ttcaaacatg gattcgcccc aacggttgaa
     4921 cacataaaat gacttcacca tggccacatt gctgccggag aacggataga aacggtcgtt
     4981 caccccgtcg tcgttgggcg agaaagccgt cgggaaatat acgtcgcggg ctttgtccac
     5041 aaacacggta atgatgtcgc tgtctttgca gccgttgcgg tcggtcaccg tcacgctgta
     5101 agtggtggtg gagagcgggc gggccatcgg attgaaacaa tcgtagcacg acagcgaatc
     5161 tccctgccaa ctgaacattt ccaccggata agaggccagc gcttccaact gaacgtcttc
     5221 tccgagcttg atactcaggt ctgtacccaa atccacaatc aattcctgcg gctggttcac
     5281 cgtcacgctg gtttgttggg tacaaccatt ggcatcctgc acccgcacga tgtatggccc
     5341 taccggcagc cctccaaaca aagagcgact gctgaacgga tcattgtcta aggcgtacaa
     5401 atacggcccc acgccacccg atacttcgcc tatagaaatg ctgccctgca cctgcccgaa
     5461 acacggcgga tcggtagccg ttgcacttac tacgatgggc ggcggaccgt tgagggtaat
     5521 gctcggcacg gcaggcgtag aagtgcagcc gttggcatcg gtgagcgtga gttgatattg
     5581 tccggcgggt acgttgcgca aggtagcctg agcgctaccc gtgctccatt ggtacgaata
     5641 aggagcattg ccgccagcca ctgtcaccgt tacttcgccg tcgtcggcgc cggggcaact
     5701 gacgttaaaa ccattgtagt tggagccggc caacgacacc gttaccggcg tgggctgcgt
     5761 cactgcaacg ttgatggttt gcacaatgcc gtagaaatca gtgactacga tgcgatagct
     5821 gcctgccggg aggttgggaa tgttcacgga cgagttattg gttgctacgt tgcccatgcc
     5881 gttcagggcg ccgcccaccg cctgccattg gtagctgtat ggaggcgttc ccaccgtcat
     5941 agagaactga acggagccgg tagaagcgcc gaaacagttg gctgcgccgg aactggaact
     6001 gaattgcagc gtacacgggt gggcggtcaa aatgagattg acgatgctgt cacagccggc
     6061 cacggaggta agccgcactt ggtaagtacc tgtggctgag aagttcatat tgcccacgcg
     6121 gaaagtctgg tcatcgcata tacgctccgt gagggtgatc tcatacaccg gattgaccac
     6181 caaattcaaa gtcacggtac tgtcgcagcc ggtcacggaa gtgagtacat ctgtgaagct
     6241 gccggctgtg gtataaacgg agctgcccac gctgaaactc tgcccctgac agatcgcccg
     6301 gttcaaagtg gtggcaatgg gagcgcgcac ggttaggttc agnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn ttggtcatcg ggatgggatg cacggtcagg ttcagcgtca
     6481 ccgtactgtc gcagccggtc acagaagtaa gcacattgct gaaggtgccg gaagtggtgt
     6541 aagaggaggt tcccaccgtg aagctctcgc cgttgcagat cgcgcgagtg agcgtggtgg
     6601 caatcggagc gcgcacggtc aggttcagat tcacaatact gtcgcagccg gtagccgccg
     6661 tgaggatggt ctggaaggta ccgctggtgt tgaatgtaga gctgcccacc gtaaaggact
     6721 cgcccgcgca gatggtgcgc accaggttgg tcatcggaat gggatgcacc gtcaggttca
     6781 gcgtcaccgt actgtcgcag ccggtcacgg aggtaagcac atcggtgaag gtgccnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnaggatg gtctggaagg tgccgctggt attgaatgca
     7021 gagcttccca ccgtaaagga ctcgcccgcg cagatggtgc gcaccaagtt ggtcatcggg
     7081 atgggatgca ccgtcaggtt caacgtcacc gtactgtcgc agccgttgga agcggtgagt
     7141 acatcggtgt aggtacctga tgtggtgtaa gtggaagtgc ccaccgtgaa actctcgccg
     7201 tcgcagatcg cctcagtgag ggtggtggtg aaggtgggat tcacggtcag gttcagattc
     7261 acaatgctgt cgcaaccggt cacggcggtc aacacatcct gatacatgcc gctggtggta
     7321 taagtggaac ttcccaccga gaacgattcg ccctggcaga tcacctgcac caagtttgtc
     7381 accggaatgg ggaacactgt cagagccaga ttgacaaggc tgtcgcagcc gtcctgtgca
     7441 ataaatacat cctgataact gcctgaaatt gtgtaagttg aactgcctat tgttacactt
     7501 tcgccctcgc agatgctgcg cgtcaggttg gtttcataaa acgaagtgat tgtaagcgtg
     7561 aggttcacga tactgtcgca gccggtgacg gccacaaggg tatcgcggta gctgccgcca
     7621 acggtgtaaa cggaggtgcc gacagtgtaa gaagccccgg tacagacgct cgtcacaata
     7681 ttcgtcaccg gaatgggatg caccgtcagg ttcagggtca cggtgctgtc gcagccgttg
     7741 gtcgccgtca gcacattggt ataaacgcct gatgtggtgt agttggagct tcccacgctg
     7801 aaagtctcgc cggcgcatat cgcctcggtg agcgtaacct gaggagtagg cagcaccgtc
     7861 aggttcaggg tcacggtgct gtcgcagccg ttggaggcgg tgagaagnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cacggtgctg tcgcaaccat
     8041 tggaggctgt caacacgtct gtgtatgtgc cggacaagtt gtatgtggat gtgccgacgg
     8101 aatacgtctc gttgtcacag atggaaatag taagtgtggt ttgcggcgtg ggcaataccg
     8161 tcaggttcag ggtcacggtg ctgtcgcagc cgttggccgc cgtgagtaca tcggtgtaaa
     8221 tgcccgttgc ggagtaggta gaggttccta ccgtaaagct ctcgccgttg caaatgttct
     8281 gtgtgagtgt ggtttgcggc gtgggcagca ccgtcaggtt cagcgtcagg aggctgtcgc
     8341 agccggccgg cgtcacaaaa aagttgtcgt aagaacccgt agccgtgtag gtgttggttc
     8401 ctaccgtata gctttcgcca ttgcaaatag atacatcgag agagcttgtc agcaggtcgg
     8461 tcaggctgag attgagataa accgtactgt cgcagccagt ggccactgaa gtaaacaccg
     8521 cctgatagct gccctccgca tcataagtat tgctgcccag cgtgtaagtc tggccggtac
     8581 aaatttccac gttcagagtg gtttctattc tgtctctgat ggtaaggttc agattgatga
     8641 tgctgtcgca gccgttgacc gccgtcaggg tgtcgctgta catgccggta gcggtgtagg
     8701 tgttcgttcc aacggtatag gattggccga aacagcgggt gatgttcagg ttggttaccg
     8761 gtacgttcag aatattcagg ttcaaggtca cggtactgtc gcagccattg gccgccgtca
     8821 gcacattggt ataagtgcct gcggtggaat atgaattgcc gcctacgctg aacgattcac
     8881 cgtcgcagat gcttcggtta agggtagtgg taatggccgg gcgcaccgtc aggttgagat
     8941 tcaccgtact gtcgcaaccg ttggcggcgc tgaaaacgcg ttgatacgca cccgatgtat
     9001 tataattagt gccgccaaaa gtataagtgt cttccgcgca aatagacaca ttcaggttgg
     9061 tcaccggttc cggcaagacg gtcaggttga gggtcacggt actgtcgcag ccattggaag
     9121 ccgtcaacac attgacataa gtacccgcat tgaaataggt gttggatccc accgaaaacc
     9181 cgtcgccggc acaaacactt tgattcagcg ttctggtgat ggcggcgcgc accgtcagat
     9241 gcagtatcac ggtactgtcg cagcccagcg aggaagcccc cggcagcacg cgggtgtaag
     9301 tacctgtggt gttgtagctt gtacctccta ccgtcaccga ctgcccgttg cagatggtat
     9361 ctctcagggt tcgcatgggg ttggataccg tgaggtacag cgtggtgttg ttcgtgcagc
     9421 cccccggccc cgttatattc tgttgatata cgccggtggc attgtaaaaa acattgccca
     9481 attgataaaa ttgcccctga caaatggtgt cgcggacggt gatgttttgc gggctgtaca
     9541 cggtgagatt gagaacgacg aggctgtcgc agccatactg attaggtaag gttaccatgt
     9601 ggggattacc gggcggactg ctggaaaaaa aagtctgatt gccaatagtg aatcctgccg
     9661 gactacaaat ttcttcgttc agaagggtaa ggtcggggtt gttgacattt atttggaagt
     9721 tgacgatgct gtcgcagccg aatgaagttt gtaagggcac actgtagctg ccgggagcgc
     9781 tgaatatctg cccgccgacc gttacgagtt ggggtacgtt gctgcaaaac tcataggtag
     9841 gcaggttggt gactttgtgc gggcgaacat tcagcgtaaa acgacgaggt tgaacacatc
     9901 cttgcggtga gacatcgttg tagtcataag tccccggcga tgtgaacacc atacctccgg
     9961 cagaataaga ctcgccttca caaatattga ctgcggggtc tgtaatcaaa gccaaattgc
    10021 caacattgac agtagtgcat gtttgagggc catctccgtg gcatacatta gacggcgtta
    10081 cgcagatagt atatgtcccg tttgtaggaa acgtgatttc tgcggtctgt gagctgcccg
    10141 ccggactgcc gttaactgtc cagttgtaaa caccggcatt gggtactccg ggagtcgaaa
    10201 aagtgtacat ggctccaggg cagtacgggg ttggaggggc tgcaatagtg ggtgctgccg
    10261 ttaccggcgg agccacggta ctgcccgaag tcacattcac cgtaaactgg cacacgtcgt
    10321 tgctgtgccc gtccaccaca aaaaagtaag taccgccaac ggtgagaccg gtcatagtca
    10381 gcgttgtagc ctggttgggg ggcgcctgat acacgcagtt ggaaaccaac gagaagctgc
    10441 tgcaatcggt ggtgccccat atccccacct gaaggccgtt gttgttgcct aagttgcaat
    10501 tgaccggagt aatgctcatg gtaatattgg aacttcccgc tacaaaacca atccattgca
    10561 cgttgtgtgg caaaatggcc tgtcctgggc aaaaagtggg cggcacctgc tggccggccg
    10621 tcgttgtatt gttcgaactg gaaaaagtgg taaagtcgca cagcacacag gaaagcgcac
    10681 aagtgttgga tagtgcaatg gtgttgggcg ggcacaaatc cggcaattgt gcgtgtgaga
    10741 tggtcgctgt ggcaaacagc aagatcgtcg caagtagaat tctcatcatt ttcaatcggt
    10801 tgtctttctt aatgaaacaa aaatggcttc tttggnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnncaaagc atggttttca tcggctttgg ggagcaaagt ttcgtgtgga ctggctttca
    10921 ggtgcgggag acaaaatgaa taagggcatt ttgtctatca ttcttgtgcg cacatagacg
    10981 cataaaaatg tcagggtgct gcctgtgttt caaaccggcg cacaaaaaac tataaatgct
    11041 gccaaaaaga aaagtaaacc gggaatttta tttgtcgaaa cccaacgccc ggcgcatttt
    11101 gtgataaatg gccgtatttt cataaacgcc cccgaacaat tccgcgcccg gcccatacgc
    11161 aaacacagga atcagcgtac ccgtgtgctg gtcggtggta aatgctcctt tgatagaacc
    11221 cataaccgag ccggggttga tggaaaaacc gccggtttcg tnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnncgatg tactgtgcgt ctttggcatg accgccccaa tctatctgag
    11401 agccttcaat catcaaaaag aagcctttgt tgctccgttt tgccaaaaaa ggcggcgcca
    11461 tgcgtgccgc aggttccaga tagtcgcgtc cgctttcggc ggtgagcggg ctgttatcgg
    11521 cactcaggta tccgaacgga tgcgcggggt caggggtgat ttgcgccagg tctttttctg
    11581 cataatgcga gatgacgtag ccttttttgc gaagttgctg caacaggtcg cgctcgtcgg
    11641 tttcccggcg gtcaaagaat tttttacccc cgccgatgaa caggtcaatg ccggaatcgg
    11701 ggaaaaaggt ggcaatggct tcctcttcgc ctctgttggc aacatgggca atgaaagagg
    11761 ccggcgttgc atgggtgata ctggaggtgg caataagccc cgtggccaag ccccgttgct
    11821 gcgcttcttc gaggatggtt tttgcgggct ttccggcggc gtccacgcct atggcgccgt
    11881 tgtaggtttt tacgccgcag gcaaaagcgg tagcacctgc agcagaatcg gtgatgaggt
    11941 cgttggaagc ataagacttg tgcaagcccg tgaccgtgca gcgctctaag ttcagttgat
    12001 tgccattggc atacaagccg gcgctgattt gccccaaccc catgccgtcg ccgatgagca
    12061 ggatgatgtt ttgggggcgt ttttgaagct ctatacttcc cgcgccggaa ggggcagttt
    12121 cggaagcagg cttgggcgcc gagcaggctg ccaatgagaa taatgcaaaa acgaacaggc
    12181 ggtaaatgcg catggcaaac ttttgttttt caatgtatta tgagcagtct ttcggctcac
    12241 cgggcaggca catagcgctt gccgggtaga gaacgaatga cgcctttaaa cnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnntga tgccggcttc aaaggtaagc gcatcaatgc
    12361 tcagttcgga gcgctcgcgg agcaaattaa caataatttg ttcctgatcg ctgagttcta
    12421 caaagagctg cggctgtata ttgcggcttt tactacgctc ttcccattgc atgatgtagc
    12481 ccagatcggc ggcgctttcc agcaaattgg cctgattggt tttgatgaga tgattgcacc
    12541 ctaaagacat tttatcgtgg aggcggcccg gcagggcaaa cacgtctttg ttgtaaccat
    12601 tggctatttt cgccgtgatg atggagcctc cgctgatgcc ggattctact accaccaccg
    12661 catcgctgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn ntgccagcaa gccgccgttt tccgtcatgc gcagggccgt
    12781 gtggcggtgc tgaggcgggt atatccggtc gaggccatgc cccaatgcag caatggtggg
    12841 catgccgttt tccaaacacc tgcgatgggc cgtcacatca atgccgaatg ccaggccgct
    12901 gatgatggtc gcatcataag ccttcagccc gtccaccaat tcttcacaaa acgccgttcc
    12961 ataatgagtc ggggtgcggg tgcctacgat gcccacggtg cgcgctgcgt tgaggtcggc
    13021 attgccgcgg tagtaaagca ttaccgggct gtctcgaaaa ggtttgaggc gggccgggnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atgccgtgct gttccataaa ttccaactcc
    13141 tgctcggcat ggcccaatac gtcctggttg atgacgtaat cggctacgag atcgccaacg
    13201 ccgggaatct tgcacagttc cttttttttg gcgcgaaaca ctgcctccac accgccgcaa
    13261 tagctgatga gcgttcgggc cgtaacggcg cccaccatcg gaatctgtgt gatggctacc
    13321 ttgtacaaca actcggggtc tttcccgtga aggtactgtt cactgctttt tgcatggttt
    13381 tgcgcaaatg agcgcggact ttttgttccc gtcatggcaa atggtgctat ttttgcatta
    13441 atttttcaac aaccttaccc aagataatga gtaccggcag aaaaaactct cgtctcgaat
    13501 tagtcgtcat cagcgtattc ttcattttat tcagcttttg ggcgctgtcc cgttgtaaca
    13561 acaaacgagc tgaaatgcgc cgccaaatgg aggccgaagc tatggacgaa cgccgtcagg
    13621 attctattga ccgcgccgct gccatggccc gcatgttgcc acccaaagat accacgccgg
    13681 cggcatcagc cacccctgcc gcgccgcagg tcatcaaaga gcaagtgacg cctttatatg
    13741 ttgttctcga cgaactgaac atgcgcggcg aacccaaact cgatgggcgc ctcgtagctc
    13801 gcctcaaact ttatgacgaa ctggtgtatc tcaacgaggt cactgaattc aaacaggaag
    13861 tcaacctggg cggcgttact tacaatgaac cctggatcaa agtcaaaaat aagcgcgggc
    13921 aggtcggctg ggtctatgga gcgggtgttc attattaccg cttccgcaga gaaagttgac
    13981 cgggcgcttc ttttgattcc tttgacaggt cttcgttaca aacagccgca aagataaatt
    14041 ttttgttttg caaatcgcaa aaaggaattt tttctatcaa aaaaaagagg cccgccgctc
    14101 gtagcctata catacactga attatcatcc ttatttccta cttttgcgcc aaaatcaagt
    14161 aaagtaaacc atgtttcaaa tcaatatcta tctgcgtttc gcattgatcg cgctgtttct
    14221 catcggcggt atcgttccgg cctttatcaa cgggcttagt tnnnnnnnnn nnnnnnnnng
    14281 tttttggtgg ggatttccgt ttattctggt cggtttgatc ttgctggcag gttatttcct
    14341 gttgggcacc atcaattcta cggcgctctt gctgcaacaa ggtaaaatag acgaggcgga
    14401 aaaacgctta gcgcttacct tctggcccca attgctgtat gtcgccaacc gctcgtacta
    14461 ttacatgatt aaaggcaata ttgcattgag caaaaaggat accgaggccg gcgaaatgtg
    14521 gctccgcaaa gcgcaggagg tcaaactgcc ttccgacaat gaaaaagcca tgctggaatt
    14581 gcagttggcc aatattgctg cctccaaagg caagtggagc caggcacaat tgcacttcag
    14641 gaatctcaaa gaccttaaaa tcaccgaaac agccatcaag gatcaggtga agcagtttga
    14701 aaaagcatta gaaagccggg gtcagatgaa agcggccatg cgcatgggca atcccggcgc
    14761 agcgggcatg atgcagccgg gaggcaaacg ccgccgcccg aagatgagat aaattccgac
    14821 aaagccgtat ccgccggggc agcctccctg agccttgggt aattacgggt gaaaccataa
    14881 tgagcggttg ccgccggcgg tactgctttc cgttgcccgt acgcggcaac gacagtttgc
    14941 atagcttatg cagacaaaat aacaggtctg tgttcgataa gtcaacgagg gcgccgacga
    15001 ttaagataag tttaacccgg ctacggcggg aggttggggc ttcgttaata aaagcataaa
    15061 aaaaacctcc gttgcagcgt attttccggt ggccggtcgt ttggcaatta taccaaaatc
    15121 actcccaata tcatgttgta ttttcatccg atgaaagtcc gtttctctat tgttttggcg
    15181 ctgctcatca ctgtgggcta catattgacc gcacaagact tccgcctcgc ctacattgag
    15241 caatataaga gtattgccat acaggaaatg gagcgcgccg gcgtgccggc cagtatcaag
    15301 ctggcgcagg ccattctgga atccgatttc ggccgcagcg atctggcccg ccgtgccaac
    15361 aaccatttcg gtatcaaatg cggcgtgaat tgggatggcc gcgaatttta caaagaagac
    15421 gatgactacg atgacaacgg caagctcgtc aaatcctgtt ttagagtata tcgcagcccc
    15481 gaagcctcgt tcattgcaca ctctgaattc ctgcgcgacc cggccaaaga ttttcgctac
    15541 ggcttcctgt tccggctcga tcctaccgac tatcggagtt gggccaatgg actcaaacgc
    15601 gccggatacg ctactgccgc caactaccat gagcgcctca tcaacctcat cgaaacctac
    15661 aaattgcatg agtacgaccg cctcggccag gcgcccgttg tggatgtcac tgtgcccaat
    15721 cagcccggcg cccccgcatt tggttacaac aacgacgttc ggttcgtcct ttcggccaac
    15781 aacgaaaccg tctctactat ttctcgccgc accgatgtgt ctctgggaca gctcattaag
    15841 tacaacgagc aactcaacga cggcgagcag cgtttggccg agggcaccat ggtatatctg
    15901 caacccaaac gcaacgcctt ccgggggcgc gcagaaagcc atttggtaaa agaaggcgaa
    15961 accatgtttg atatttctca aacctacggc atcaaattgg ccaaactcct ccaacgcaac
    16021 cgtatggaaa aaggcatgga acccgctgtc ggtcagaata ttaaactgcg cggcggcaaa
    16081 gtaaaacaac gccctgccct tcgcggggaa gccaccctgg acaagcccgt caacggagtg
    16141 cgcatagaaa tggaggaagc gcccgcaccg cctcctgccg cccccacacc gccccccacc
    16201 gtacagcccg aacgggaatc cggcatccga ccggtgacgc cggctcctac gcccacaaca
    16261 cccgccaata ctcagcctgc cgacacaccg ccgctggccc ctgtgaaacc ggaagagccg
    16321 cgcccaagta cgcccgttgt acagcctgcg ccctctaccc cagtcacaac gccgccgccg
    16381 gccactgcgc cggtatatca cacggtagaa aagggcgata cgttgtggaa catctcgcag
    16441 cgctaccaga ccaccgtggc cgccatccgg caactgaaca acatgaccaa cgatagtatt
    16501 caattgggcc agcgcctgcg tgtgaagtag aatttgttcg ctatctttgc cgctcagtat
    16561 cggcgctcca ttttcatcgc agcgctgatg cgctcaatac gttcggcatg aaaaaacttc
    16621 tcgtatttgt cgcaatcatc atggccgctc aggggctgtg ggcgcagggc tatgtgttcg
    16681 gtattaaagg cgggcctact ttcgggcttc aaaactgggg caattcattc cagcgagatc
    16741 ccttgattcg ctaccacgga gacgtattca tcgagtctct gcccgaaagc aaccaatggt
    16801 ctttgtttgc gcaggcaggc taccatgcgc ggggcagcgc catcagaaat cgcaatttca
    16861 tcaaccctct caacggcaat ttcttccgcc cgccggccga tgtgtttctc tttcacaatg
    16921 cagccattgt gctgggcgcc aagcaaaaat tcgatctggg tctgagcagc aaatggtttt
    16981 acaccttcgg cgtgcgcggt gaatatacca tagatacgaa tttggacagg tacgatgctt
    17041 ttttggaact caatccaagt attgctgcgg tttatcctat caatgcctat tttgaagaca
    17101 atggcctgtt gggtatccgg cgcatcaatt atggtttttc aggcggcgcg ggtttggagt
    17161 ggcagttctc ggagtttctc ggcgcttcgt tggaatttac catcaacccc gatttttcca
    17221 atcaatacac acagccggcc attcccaacg tacgcgatcc ctggacgggc gaactgcgca
    17281 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gaggttaccg
    17341 ccgccttccg gttcttgcgc aaaattgaat acattgacta aaacgacccg cctacaagta
    17401 tgatcctacg ttgcgaaaat ctgaaaaaat tgtacggacg ccggcaggtg gtgcatgatg
    17461 tttctctgca tgtcaacaaa ggcgaaatcg ttggtttgct ggggcccaac ggcgcaggca
    17521 aaaccactac cttttatatg acggtaggat ttattaaacc caatgagggg cgtgtctttc
    17581 ttgataatca agatattacc gatatgccca tgtatcgccg cgcacaacac ggcattggtt
    17641 atctgccgca ggaagcttct gtttttagaa aactgagcgt ggaagacaac gtgcgggctg
    17701 ttttggaaat gactcatctg aataaaagtc agcaaaaaga aaagttggaa tcgctcatcg
    17761 aagagttcgg cctcgaaaaa gtgcgccgca gccggggcga taccctctcc ggcggcgagc
    17821 gaagacgtac cgaaatagcc cgcgcattgg ccaccgatcc cagtttcatt ctgttggacg
    17881 agcctttcgc cggtattgac cccattgcag tagaagacat tcagtacatc gtggccaaac
    17941 tgaaaaccaa aaacatcggg attctcatca ccgaccacaa cgtgcaggaa accctctcca
    18001 tcaccgaccg ggcctacctc atgtttgaag gcggaatcct gcgtgccggc accgccgaag
    18061 aattggccga agacgaaatg gtgcgcaagg tgtatctcgg ccagcatttc gaattgcgcc
    18121 gtaaagtcat acatgtataa tgcgctcgcg accatatctg ctgccgattt ttgtgtgtgc
    18181 cgctctcctg tacggaggct gcaccgacga cgctcccacc cggctcagtt ctgatcaact
    18241 gcaattgctc gattccttgg tcacggtgca aatgccattg gtacggcagg aggaagacag
    18301 catctgcaag gcggattttg ataaaaaact gcgccgtatg gtggattcca tgctcgtcat
    18361 tcgccgcgaa gaagaagccc gcctgcgtca aagaatgccc aaacaatgag ccggtatagt
    18421 tacatcttcc tcctctttgc cgcactgtcg gttgttgccg tctcctgtga tgactcggcc
    18481 aaacaaaaag cccaggagga agcgctcctg caaagccgac tgctggaacg cttggaaatg
    18541 gggcgcgtca cgcgtcggga agagtgcatg cggcaattgg ccgattccgc tctggtcatc
    18601 gttgattcca ttcgcttaga agacgcccga tgggccttgg atacccttnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnncct gggcctggcg tatctgtgga ttcattggaa
    18721 gtaaaaccgc tgctgccgga ttccatcaaa cgacgcaaca attgatcttc aacattttgt
    18781 cattgccaat tcattgatat tatgatcgta cttaaactca ttgtaggttt aattttgctc
    18841 gttgtcggcg cggaagcgtt agtcaggggc gcctccagaa tagccgcatc atttggaatt
    18901 accccgcttg ttatcggcct cactgtggta gcattcggta cgagttcgcc ggagctggcc
    18961 gtgagtgtaa tgtcttcctt gtcaggtgct tccgatattg ctttgggcaa tgtggtgggc
    19021 agtaatattt tcaatgtatt gtttattctg ggcatctctg cgctcatcat tccattggct
    19081 gtgcaccagc aaatcatcaa aatggatgta cccattctca tcggcctttc gctgctgact
    19141 tggtggatgg cctccgacgg caatattcag gtttgggaag gcagtatatt gattttgctg
    19201 ggaatcgctt acatgctcct gctcatccgc atgagtaaaa aggaaaaaaa agcgatagaa
    19261 caggagtacg acgaagcctt cggagagcaa aagggcaaat ccggcaaaat ctggatgtcg
    19321 gcactttttg tactcgccgg cctggctttg ctggtattag gctccagatg gttggtggac
    19381 ggagccgttt atattgccaa aagcctcggc gtcagtgatt tggttatcgg ccttacgatc
    19441 atcgcagcag gtacatcctt gcctgaagtg gccacctcca tcatcgcagc cttgcgcggc
    19501 gagcgcgata ttgcagtggg caatgtggta ggcagcaacc tgtttaatat cttctttatt
    19561 ttgggaatag ctgccgtaag cggcggcggc atcggcgtag cagactcggc cattcgtttc
    19621 gatctgccgg tgatgacggt ggtagcgttt gcctgtttgc ccatcttttt cacaggaagt
    19681 aagctcgaca gatgggaggg cgctgtattc ttgttgtatt acattgccta cacagcttac
    19741 ctcatcttag ccagccaaaa cagctcgtct ttggaggcgt acagccacgc cattctttgg
    19801 tttgccgtac caattaccat actaacgctg ggcattgtga cctggaggca agccaaacgc
    19861 aataaagcag tttaatgaca tttcgagact gcccgaagcc atcaacaggt tagatcgctt
    19921 cgggcaatct aaagtgaact tgagataatg aatgaaaggg aaacggggtg aagaaaaaag
    19981 agtggtgcga gacggaatcg aaccgccga
//
LOCUS       DAORLF010000029        19626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1492_length_19626_cov_2.119003, whole genome shotgun sequence.
ACCESSION   DAORLF010000029 DAORLF010000000
VERSION     DAORLF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19626
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_19626_cov_2.119003"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..592
                     /locus_tag="PK198_04335"
     CDS             158..592
                     /locus_tag="PK198_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37996.1"
                     /translation="MTEYEEIIIRWKPRIKWAADKFEKMEAKNVLYQIYCDSHLYGRD
                     VLAYIGKTDRSFGQRMDEHFKSFFQYANNVNYSVGEIMQYTGKLEIPESILIANHKPF
                     FNKEYIHDLPLEAKKQKIIVINEGEFGMLKSCCTNYWWCEKK"
     gene            647..1531
                     /locus_tag="PK198_04340"
     CDS             647..1531
                     /locus_tag="PK198_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF37997.1"
                     /translation="MAKKLIRFDWAMKKMLRHKANFGILEGFLSELLEEEIKIKQILE
                     SESNKETEDDKSNRVDILVENSKGELVIIEVQNSKEYDYFHRMLFGAAKAITEHIKEG
                     QAYAEVKKVIAITIAYFDLGQGKDYVYHGTTVFKGIHKHDILTLADKQIELYKKNNIH
                     EIYPEFWVIKVGKFGNRVKDKLDEWIYFFKNAEIKADFSAKGLKEASERLDEMKLNEK
                     DAKEYKRYLKGLRDIASQQHTKMADAQDLINKGKEDKEIEVVLEMHKDGVSTKTIAKY
                     LKITEERIAQILENQRNK"
     gene            1646..2482
                     /locus_tag="PK198_04345"
     CDS             1646..2482
                     /locus_tag="PK198_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37998.1"
                     /translation="MKWFWNLLLILLVILLIYFGLAYFGIIRDPVVINKLSEHFGGSR
                     YFTPACFLKDLSGCYQLKLRMRDGELPSFDKILEGNISKTQCNLSGYIYSDETSKWRL
                     IGEIFEAEDRIEIKIVKQTSSFEDSLSLITFNNIGVRQIESLSGTVENEEGRAIGRAK
                     LIRCLNKVSEHEEDVYNNTKENIKTNHVYYVQVLCSSNYKAIEREVREKMSEFISGNL
                     QLHIKNNQAANCTRMMIGEFNDISSAKIFRDKYDLPYGRGVIVECFEGSDGKEIISTI
                     TE"
     gene            2681..3154
                     /locus_tag="PK198_04350"
     CDS             2681..3154
                     /locus_tag="PK198_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF37999.1"
                     /translation="MNTQEGIKLSSNWTNVRLYVSCALALLMIPVMILVTQEQEFHIG
                     MLIGGIIFLVFTGFVIYQFIYVCDARVVGGKVVLRKKFRPAKTYSFDKIGYPTSFQLK
                     SIKYTTVEMKNDDNTTEKYLIISSKSFLSFENNDAEHILLSLRDMAKNNNKSPFS"
     gene            complement(3182..3931)
                     /gene="rlmB"
                     /locus_tag="PK198_04355"
     CDS             complement(3182..3931)
                     /gene="rlmB"
                     /locus_tag="PK198_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRF38000.1"
                     /translation="MRREEQDQNIIFGRHPVLDALNAGQEIDRVILQQGTRGEFEKEI
                     RHACRRSDVLLQVAPKERLDRLVRGNHQGVVAYAALIQYQPLDDVLASVFERGETPLL
                     LMLDSITDVRNLGAIARSAECSGVHALILPAKGSAQINAEAIKTSAGALHNLPVCRVN
                     SLVSTMQRLQDSGVKALAGDLQAEHLLFETDLTGPLLLIAGSEGEGVSQALLSRINER
                     FIIPQAGTTNSFNVSVATGIMLYEVMRQRRG"
     gene            3996..>4265
                     /locus_tag="PK198_04360"
     CDS             3996..>4265
                     /locus_tag="PK198_04360"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRF38001.1"
                     /translation="MTTLAYITWNALPEIAELGPLTLRWYGLLFALGFLLGLFMVRRM
                     FLAENAPEAWLDKCFIYVVAGAVLGARFGHVFFYDWDYYSQHPGEM"
     assembly_gap    4266..4282
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4283..>4700
                     /locus_tag="PK198_04365"
     CDS             <4283..>4700
                     /locus_tag="PK198_04365"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRF38002.1"
                     /translation="EGGLASHGGAIGIILAMWIFSRTVSKKSILWILDKIVVPTALAG
                     GFIRLGNLMNSEIVGKPTDSGIGFLFKNAARDTWVKCEECAQIASSCVKTGFNDMGAE
                     LCMVPRHPVQLYESISYFLIFAVLFYTYWKTDKRHKP"
     assembly_gap    4701..4722
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4760..4891
                     /locus_tag="PK198_04370"
     CDS             4760..4891
                     /locus_tag="PK198_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38003.1"
                     /translation="MWEYFKMSQGGFESAFGNVLSTGQLLSIPFVLIGLYFMFRKTK"
     gene            complement(4980..7058)
                     /locus_tag="PK198_04375"
     CDS             complement(4980..7058)
                     /locus_tag="PK198_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF38004.1"
                     /translation="MAEGNSRGLDFDLWFHFGELYAAQGNLAQAHHYFNRAFDRRFQV
                     FGLKHYKIAEVFSAYGELAARQNDYACALEHYQSALISLTEGFDETDIRQNPVRFQST
                     FIHKDIVSVLGRKIETLRRWAAVSENDEYLLSAWATVQAAVQAVDILKTGTLQSEDDK
                     LLFFRQSAQIFEHGIQIAWELGPKFHAAAFELSEKSKAIVLLDAFRNANALRLAGIPD
                     SLLEREAQLKYEISAAEDTRFKGDQSPELRDRLLSLQNEYRALLSHFEQNYAQYYRLR
                     YDHAVISSSDAQQSLRRGQSAVAFFCGTQSLFAFVLHSDGMTMYQLPSGEPIDALVTN
                     MRAGIYDYFLASQRTAAVYESSAAQYVQSASRLYDVLLAPFAAMLPEDVLFIPDGVLG
                     YLPFEALLTAPPPGSATRFKTHPYLLHRHRISYCYSMTLWQEMQQNPSANRKLAAFAP
                     AFRPAASLQPMALRGALDTLYFNRPEVEEIASVLKQAGAYSGRQAVKSQFMQIAPHAG
                     ILHIASHGIANDTASGYSFIAFTSADPSSDSSRLYARELYNLRLNAAMVVLSACETGI
                     GELKKGEGIISLARAFAYAGAQSVVSTLWSVNDESTTHIMRAFYKDIRAGNTKSEALR
                     NAKATYIRHSAEDMAAHPFYWSAYIAVGDMRPLYRARVWLMALGILVLAMTGLGFWRR
                     SRMKAQTQES"
     assembly_gap    7439..7557
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7558..7796)
                     /locus_tag="PK198_04380"
     CDS             complement(<7558..7796)
                     /locus_tag="PK198_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38005.1"
                     /translation="MHKKVLEELIPACEANSDSPCIFDHLKKLTRKLEDAQSWKMYVD
                     ILSVQIEYARQWDRLDIFYETLKTAQKVIPEKKKHL"
     gene            complement(7975..8577)
                     /locus_tag="PK198_04385"
     CDS             complement(7975..8577)
                     /locus_tag="PK198_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38006.1"
                     /translation="MRTTLLFPVAVTVVLAVFLFACEPPPLDMNATLSIHQLLADDIQ
                     AELDSLETTPGTENDAIETDVPITEIEISKEREVLEEQMKYERTNATYINEQLCNGIP
                     NCPPRALSRIGEPCGKGKCYDLSLFKRWACRFELQLKVSIYDAQNNLVFESLPGNEKE
                     KYEGNSGIRIITLPENLAAGQLYRIDFQEGGRQRSTFASF"
     gene            complement(8644..9435)
                     /locus_tag="PK198_04390"
     CDS             complement(8644..9435)
                     /locus_tag="PK198_04390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38007.1"
                     /translation="MTDTYTLMEKYLDGTLAGDELSTFEAQLRKDKILQAELSMQRQG
                     RAALGAVADSEVADFEKILKKNSAHYFPVESQPAGTISKAAPRIVWRKWLAAAAAVLA
                     LVFAGLKWYATAKYEISAILAQHYAPAATPATLSGETYDLQEAYTAYRNQKYAVAIQA
                     FNAAPADDPQYTEAMLFKGYACYESGQYGAALAAFDLVIKSSDARFLHNAEWHRLLAA
                     LAENPKSEPAQAYLQQILGDASHPYYAEAKKIERQLKSPLRYLAG"
     gene            complement(9439..10044)
                     /locus_tag="PK198_04395"
     CDS             complement(9439..10044)
                     /locus_tag="PK198_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF38008.1"
                     /translation="MSTQHIPHDDQYILEALVQHDSSVVAAVYRDYSDMIRALVTENG
                     GATNDAGDVFQDALIDIYRQAQRGYVLTCSFKSFLYTVCRNKWLDELRRRGRMEVTIK
                     GSERFTTEIAEDNADRLDQHEERLALLAATVETMGPSCRDILKLAWTPHSQTNKYPAL
                     SEVAEQLGLSYAYLRKKKSECEQALREKMKNAPEFTQLKDQ"
     gene            10180..10662
                     /locus_tag="PK198_04400"
     CDS             10180..10662
                     /locus_tag="PK198_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38009.1"
                     /translation="MKTIQKMILMTGVVLLVLAGISMSGCKPDPIEPAKILSLDITLR
                     TGGDDLRQGSEALGTIGFKNGSNTSISLNNGANWGNNSTNSVSMTMPPNTEVGSLNWF
                     KVNFTSGSGGFTGDNWNLDQIKITARLSDGSNLILAERTGSPLVRFTQNEVREYTVSF
                     "
     gene            11081..12061
                     /locus_tag="PK198_04405"
     CDS             11081..12061
                     /locus_tag="PK198_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38010.1"
                     /translation="MMKSLLFSALFGLLFLSLSAQPTKGAWMLGGDARFQSNIGGNSF
                     NALSLSPRVGYFVSDRLALGMGLGLAVLNTEFANYDGGNVSPFARYYFPLKKSKLSFL
                     LETGGSFSTITIGGETTNTWRVQAGGGINYFLTPEFALEGMLGAGRVFPTAGDHFTET
                     DFRLGFQVYLHRKSSDDDAPIGSGALRKRSLLLGVARTLTLNSDNQRFEPAFSYMLSD
                     RLAVGAAITLPINEFRPHLRYFTGSENASRLRPFLGAGSSFIWEYGESDQFNITPFAD
                     AGVLYFISPNIALEGALQYQADFRAFRPASDLTSNLMARVGFQYVLPGKR"
     gene            12101..13576
                     /locus_tag="PK198_04410"
     CDS             12101..13576
                     /locus_tag="PK198_04410"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRF38011.1"
                     /translation="MDTITPTPQAGWHFDNSYAALPDIFRAELPPMPVQAPRLLLFNR
                     TLALELGLQPEVLESDYGAAVFGGNQLPQGARPIAQAYAGHQFGHFAMLGDGRAILLG
                     EQITPDGKRFDIQLKGSGRTPFSRSGDGRAALGPMLREYIISEAMHALGIPTTRSLAV
                     TLSGEAVYRETAMPGAILTRVAASHIRVGTFEYVAARREPESLRTLADYVIRRHYPLM
                     ADAENPYLALLRAFIDRQAALTASWLAVGFIHGVMNTDNMALSGETIDYGPCAFMDDY
                     HPAKVFSSIDTQGRYAFGNQPNVAQWNLVRFAETLIPLLHAEEAQAVALAEEAIEPFP
                     RLFRHHWMLMMRAKMGFYNREPDDVDLLKELLEWMEQQHADYTNTFRDLSRAELPELL
                     AYKEAAFAQWHKKWLARVARQPEPMEDIRRRMRQTNPAIIPRNHKVEEALTAAAQRGD
                     LGPVQQLLAALARPYEELPGLEAYRLPPAPEEVVEQTFCGT"
     gene            complement(13747..14592)
                     /locus_tag="PK198_04415"
     CDS             complement(13747..14592)
                     /locus_tag="PK198_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF38012.1"
                     /translation="MIVIPSFIVLAAAIYGGACAVFYFLQDYFFFRPEILPQHFEYKY
                     PFPFTEVNFEMEDGGAVNGVHFKVPNSKGVVFYLKGNSRSIKGWGKFARDFVSKGYDF
                     FMFDYRGFGKSSGRRTETTLYNDAQTVYKWLSTQFPEDRIIIYGRSLGSGIAARIASW
                     NKPQMLILDSPYYSFLYQIRRYGFWMPLQRLLRFQLRTDQFMRKITCPVFILHGNKDR
                     LISYEQSVMLQKLCPDRCALLTIPNGRHNDLPEFAEYHEYLYDILNDPGYFYEVQGGK
                     SARVA"
     assembly_gap    14637..14650
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14651..16523)
                     /locus_tag="PK198_04420"
     CDS             complement(<14651..16523)
                     /locus_tag="PK198_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRF38013.1"
                     /translation="MGEQKVSVAQEGTGMQSFVRSLLRDVQALEYMLENDWFEKDIIR
                     IGAEQEMCLVDAGTYKPACINMEVLEKLSQYPWVVTELAKFNLEVNLSPREFTGKCLR
                     EMDHENREYLNIIRQQLKHWNAEVCLTGILPTLRKPDLDLSNLTPKPRYFALMEALNS
                     QMLGSAFELRLSGIDELLVKHDSPLLEACNTSFQVHLQVSPDLFVPMYNMAQALAAPV
                     MAISANSPIVFGKRLWHESRIALFQQALDVRTTHDHMRERSPRVSFGTGWLEGSLLEI
                     YKEDIARFRVLLAGDVDEDSIEAIQAGRTPKLRALQVHNSTVYRWNRPCYGISPNGQP
                     HLRIENRVIPAGPTTTDAAANAAFWLGAMIGMGHEYKDIRNHVSWEDVRDNFMKAAQF
                     GIDSHFNWFHDKKLSACDLVLHELLPMARKGLQMRGVIKQDIDKYLGIIEQRARDHMN
                     GARWQLRTFTKLAKECTRDEAVTAMTAAIVHNQWKDIPVHQWTMPAFSDLPNYHPSKL
                     RVEEFMETDLFTVQKDDLIELVTDLMDWRKIRYMPVEESKGQLCGLITSRLLLRHFAR
                     KNKLNSKEATMVKDIMIEAPITISPNATLLEAMNLMREKRIGCLPVVQNDELIGIITE
                     "
     gene            16740..17270
                     /locus_tag="PK198_04425"
     CDS             16740..17270
                     /locus_tag="PK198_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38014.1"
                     /translation="MRAVLYLSIFGLLVSACRQDNGERVFEMVFPNITFEIPAGLNAT
                     FQQVFQIPLVPTNIDTYLAENGTDTALITAIAPFTATLTSLEGAAFDYDFVEGISIRI
                     CDPVKPACFDGDEVFYIDNLQGRGGQTIRLIPGLRNAKRDLVRRHFRMEAVFFFAYTT
                     PYSVRSRLQMNFEALK"
     gene            complement(17280..>17694)
                     /locus_tag="PK198_04430"
     CDS             complement(17280..>17694)
                     /locus_tag="PK198_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRF38015.1"
                     /translation="GYTVNVPLPPGSADDIFLDAVNRFLPVAELFQPDVVALSAGFDA
                     HQYDLLLQLNVSATGYYKLGKLLAAKFPRMFATLEGGYNLEWLRNSAYNFVAGINGEA
                     IVYPEPETSSGLRVWEEYEIRANMAISHLSKFWKV"
     assembly_gap    17695..17763
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17764..18250)
                     /locus_tag="PK198_04435"
     CDS             complement(<17764..18250)
                     /locus_tag="PK198_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRF38016.1"
                     /translation="MKFLYNPIYLDHDTGMHPESMRRLQMFTNLPETPLVDGSEYLHL
                     VHTPRYIEKVRDACDLGGHLDADTVTSPGSFQAAIHAVGATIAASETGDFALVRPPGH
                     HAYPNRASGFCLFNNVAIAAAKLRSEGKRVLIFDFDGHLGDGTSDMFYNDDQVMYWSI
                     HQ"
     gene            complement(18403..>18724)
                     /locus_tag="PK198_04440"
     CDS             complement(18403..>18724)
                     /locus_tag="PK198_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRF38017.1"
                     /translation="RGDFTRGIYISSIISTDRPLQEIQALYRNFYADAPFTHVSDAMI
                     DLKQVVNTNKCLIHTELHDGKLVVHSAIDNLLKGASGQAVQNLNLMMGWQETAGLGLK
                     GTGF"
     assembly_gap    18725..18902
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18903..>19228)
                     /locus_tag="PK198_04445"
     CDS             complement(<18903..>19228)
                     /locus_tag="PK198_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRF38018.1"
                     /translation="FSNAIDTAAEVLFLCSGHHEARKFLESNHIPDSTRIIDLSQDFR
                     LEKQARIGSRSFVYGLPELHREAIRQARSIANPGCFATAIQLGLLPLAKAGILGETYS
                     TGITGS"
     assembly_gap    19229..19415
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19416..>19626)
                     /locus_tag="PK198_04450"
     CDS             complement(<19416..>19626)
                     /locus_tag="PK198_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRF38019.1"
                     /translation="LLENSQQKVSGDVFVQLFPYRFQINGIESPHDLMSSRFGSYGEM
                     NSGWTGEDVRGFSKIFGNQTAIYHAV"
BASE COUNT         4736 a         4711 c         4866 g         4707 t
ORIGIN      
        1 gggtaagtaa aatgcgcaat gcgcctttcg atatatccgt tgtggcgtat ataaacccgg
       61 cgcgaggcgc atacacgcca ttgcgcctat ggaggggttg acgagtatag acgagatggg
      121 cgggactaaa aataaaaaat aaattaaaaa tattaatatg acggaatacg aagaaatcat
      181 cattagatgg aagccaagga taaaatgggc agcagataaa tttgaaaaaa tggaggcaaa
      241 aaatgttttg tatcaaatct attgcgattc tcatttatat ggcagggatg ttttagcata
      301 tatagggaaa actgacaggt ctttcggtca aaggatggat gaacatttca agtcattctt
      361 tcaatatgcc aataatgtaa attactctgt cggagaaata atgcaatata ctggtaaatt
      421 agaaatacca gaatcaattt tgattgcaaa ccataagcct tttttcaaca aagaatacat
      481 ccatgacctg ccactcgaag caaaaaaaca aaagataata gtaatcaatg agggtgagtt
      541 tggtatgttg aaaagttgct gtactaacta ttggtggtgt gaaaagaaat aaaaatacta
      601 tcgttaactc gtaatatttc gtatttttgc atcaaaattt gcaaaaatgg caaaaaaact
      661 aataaggttc gattgggcga tgaaaaaaat gcttcgccac aaagcaaact tcggcatcct
      721 cgaagggttt ctctccgaat tgttggaaga agaaatcaag ataaagcaaa tccttgaaag
      781 cgaaagcaac aaggaaacgg aagacgacaa atccaaccgt gtggatattc tcgttgagaa
      841 ctcaaagggt gaactcgtta ttatcgaagt ccaaaatagc aaggagtacg actatttcca
      901 tcgaatgctt ttcggtgcag ccaaggctat cacagagcac atcaaggagg gtcaagcgta
      961 tgccgaagta aaaaaggtaa ttgcaattac aattgcatac tttgatttag gtcaaggcaa
     1021 ggactacgtt tatcacggaa cgaccgtttt taaaggtatt cataagcacg atattctaac
     1081 tctcgctgac aaacaaattg aactttacaa aaagaacaat atccacgaaa tctaccctga
     1141 attttgggta atcaaagtgg gtaaattcgg taatcgggta aaggataaac tcgacgagtg
     1201 gatttatttt ttcaagaacg cagagattaa agcagatttt tcggcgaagg gtttgaaaga
     1261 agcaagtgag cgacttgacg aaatgaaact gaacgagaaa gatgcaaaag aatataaaag
     1321 gtatttaaaa gggcttcgag atattgccag ccaacagcat acaaaaatgg ctgatgcgca
     1381 agacttgata aacaagggca aggaagacaa ggaaattgaa gtagttttgg aaatgcacaa
     1441 agatggtgtt tcaactaaaa ccattgccaa atatttgaaa atcacagaag aacggattgc
     1501 tcaaatcctt gaaaatcagc gtaataaata aaccgcccat caacggcttt acgaaaatgc
     1561 gctgcaaaac ccacccgcaa aagccaacgc agcagcgcac attcgtaaag cccaacgtta
     1621 ggacgtaata gaaataataa acaatatgaa gtggttttgg aatttgctgt taattctatt
     1681 agttatttta ctcatttatt ttggattagc ctacttcggt ataattcggg atccggtcgt
     1741 tataaataaa ttgtctgagc attttggagg aagtagatat tttacaccgg catgttttct
     1801 gaaagacttg agtggttgtt atcaactgaa actacgcatg agagatggag aactcccctc
     1861 ttttgataaa atattagaag gtaatatatc aaaaacccaa tgtaacctta gtggatatat
     1921 atatagtgat gagacaagta aatggagatt aatcggagaa atatttgaag cagaagatag
     1981 aatcgagata aaaattgtaa aacaaacatc atcgtttgag gatagcttat ctttaatcac
     2041 atttaataac attggagtta ggcaaataga gtctttaagt gggactgtag aaaatgaaga
     2101 aggaagagca attggacgag ctaaactgat taggtgctta aacaaagtta gtgaacatga
     2161 agaagatgta tataataata ccaaagaaaa tataaaaacc aaccatgttt attacgttca
     2221 agtattgtgt tcctcaaatt ataaagcgat agaacgtgaa gttagagaaa agatgtcaga
     2281 atttatttcg ggaaatttac agctccatat aaaaaataat caagctgcaa attgtacaag
     2341 aatgatgatt ggtgaattta atgacattag ttcagcaaaa atctttagag ataaatacga
     2401 tttgccctat ggtagagggg taatagttga atgttttgaa ggctcggatg gtaaagaaat
     2461 tatatccact attactgaat aaatacatct aacgtcgatt ttgattgggc aatgaagcgc
     2521 ctgctctgca acaaagccaa cttcgacatc ctggaaggct ttctgtcgga gctgctgcgt
     2581 gaggatttca aaataatcgt tttgcggaaa ttgctttaaa tatcgcatct ttgtacagcg
     2641 taaagtacgc tgttgcgaaa accaagtaaa ataaaaaaac atgaacacac aagaaggaat
     2701 taaattgtcg tctaattgga caaatgtcag actgtatgta agctgcgccc ttgcattgct
     2761 aatgattcca gtaatgattt tagtaactca agagcaagaa tttcatattg gcatgttgat
     2821 aggaggaata atattccttg tattcacagg gtttgttatt taccaattta tttatgtttg
     2881 cgatgccaga gtagttggag gtaaagttgt tttaaggaaa aaattcagac cagctaagac
     2941 ttactcattt gacaaaatag gataccctac atcgtttcag ttaaaatcaa taaaatatac
     3001 cacggttgaa atgaaaaatg atgacaatac aacagaaaaa tacttaatta taagctctaa
     3061 atctttcttg tcatttgaaa acaatgatgc agaacacatt ttactgagct taagagatat
     3121 ggcaaaaaac aataacaaat caccattttc ataaggggcg ataaaaaaat aatcctaaaa
     3181 ctcaccccct ccgctgccgc atcacctcat acaacataat gcccgttgct accgacacat
     3241 tgaaagaatt ggtagtaccc gcctgcggaa taataaaacg ctcattgatg cgcgaaagca
     3301 gcgcctggct cacgccctcc ccctccgaac ccgcaatcag cagcagcggc cccgtgaggt
     3361 cggtctcgaa aaggagatgc tcggcctgca aatcgccggc caaggccttc acccccgaat
     3421 cctgcaggcg ctgcatggta ctcaccaagc tgttcacccg gcaaacaggc agattgtgca
     3481 gggcgccggc agaagttttg atggcttcgg cgttgatctg ggcgctgcct ttggcgggca
     3541 atatcagggc atgaaccccg ctgcactcgg cgctgcgggc tatggccccc agattgcgca
     3601 catcggtgat gctgtccaac atgagcagca ggggcgtttc gccgcgctca aacacggagg
     3661 ccagcacatc gtccaagggc tggtactgta tcagggccgc gtaggccacc accccctggt
     3721 gattgccccg caccaatcgg tccaggcgtt ctttgggcgc cacttgcagc agcacgtccg
     3781 agcggcggca ggcatggcgg atttctttct caaactcccc gcgcgtgccc tgttgcagga
     3841 tcacccggtc aatctcctgc ccggcgttca gcgcatccaa taccggatgc ctgccgaata
     3901 tgatgttttg gtcttgttct tctcttcgca tcggccaaaa gtaggcaatt attccctgaa
     3961 aagctctaag ttagcgccct caaaactaca tagccatgac gacactcgcc tacattacct
     4021 ggaacgccct ccccgaaata gccgaactgg ggccgctcac gctgcgctgg tacgggctgc
     4081 tctttgcact gggtttcctg ctgggcctgt tcatggtgcg ccgcatgttt ctggccgaaa
     4141 acgccccgga agcctggctc gacaagtgct tcatctatgt ggtagccggc gcggtgctgg
     4201 gcgcgcgctt cgggcacgtc ttcttttacg actgggatta ctactcgcag catcccggcg
     4261 aaatgnnnnn nnnnnnnnnn nngggagggc ggcctcgcca gtcacggcgg cgccatcggc
     4321 atcatattgg ccatgtggat tttctcgcgc accgtctcca aaaaatccat cctctggata
     4381 ttggacaaaa ttgtggtgcc caccgccctg gccggcggct tcatccgcct cggcaacctc
     4441 atgaactccg aaatcgtagg caaacccacc gacagcggca tcggtttctt gttcaaaaac
     4501 gccgcccgcg acacctgggt gaaatgcgaa gagtgcgctc agatcgcctc ctcctgcgtc
     4561 aaaacgggct tcaacgacat gggcgccgag ctgtgcatgg taccccgcca tccggtgcag
     4621 ttgtacgaat ccatcagcta tttcctcatc ttcgccgtgc tcttctacac ctactggaaa
     4681 accgacaaac gccacaaacc nnnnnnnnnn nnnnnnnnnn nntgggttct ttttggtgct
     4741 gatctggggc gtgcgcatcg tgtgggaata cttcaaaatg agtcagggcg gcttcgagag
     4801 cgctttcggc aatgtgctca gcaccgggca gttgctcagc attccctttg tactcatcgg
     4861 cttgtacttt atgtttcgga agacgaagtg ataagtaccg gcgggcttga gtagcgtgta
     4921 attaagtatc tacaagaatt ttgctcaaat tttctgattc aaagcccttg ataaaaaggt
     4981 catgattcct gggtttgagc tttcatcctg cttctccgcc aaaaccccag gcccgtcatt
     5041 gccagtacca gtatgccgag ggccatcaac cagacccttg ctctatacag cggtcgcata
     5101 tcgccgacgg ctatgtatgc cgaccaataa aaaggatgtg cggccatatc ttcagcgctg
     5161 tgccggatat aggttgcttt ggcgtttcgc agggcttccg atttggtgtt gccggcgcgg
     5221 atgtctttgt aaaaagccct cataatgtgg gtagtagatt cgtcattcac cgaccagagc
     5281 gtggaaacga ccgactgtgc gcccgcatac gcaaaagccc tggccaaact gatgatgccc
     5341 tccccttttt tcaactcgcc gatgccggtt tcgcaggcac tgagtaccac catagctgca
     5401 ttgaggcgca aattgtacag ttcgcgggca tagagccggc tgctgtcgga gctggggtcg
     5461 gcagacgtaa atgcgatgaa agaatacccc gatgccgtgt cgttggcaat accgtgcgat
     5521 gcaatgtgca ggataccggc atgaggcgct atttgcataa actgcgattt gacagcctgc
     5581 ctgcctgaat atgcgcccgc ttgctttaaa acggaagcga tttcttccac ctccgggcga
     5641 ttgaaataca aggtgtctaa ggcgccgcgc agagccattg gttgcaatga agccgcaggt
     5701 cgaaaagcag gcgcaaatgc tgccagtttt cggttggcac tcgggttttg ctgcatttcc
     5761 tgccatagcg tcattgaata acaataactg atgcgatgcc gatggagtaa ataaggatgg
     5821 gttttgaaac gagtagccga ccccggcggc ggcgctgtga ggagcgcttc aaacggcaga
     5881 taccccagca ctccgtcggg aataaagagc acgtcttcag ggagcattgc tgcaaaaggc
     5941 gcgagcagta catcataaag ccgggaggca ctttgcacat attgcgccgc cgaggattca
     6001 tacactgcgg cggtgcgctg agaagcgaga aagtagtcat aaatgcccgc ccgcatgttg
     6061 gtgaccaacg catctatggg ttctccggat ggaagttgat acatcgtcat gccatctgaa
     6121 tgcaggacga atgcgaacag gctttgagtt ccacaaaaaa aggcgacagc cgactgccct
     6181 ctgcgaagcg attgttgagc atccgacgaa gagatgacgg catggtcata ccgcagacga
     6241 tagtattgtg cgtaattttg ctcaaaatga ctcaataatg ctcggtactc gttttgcaga
     6301 gacagcaaac gatctcgcaa ttccggactc tgatctcctt tgaaccgggt atcttcggcg
     6361 gcggatattt cgtatttgag ctgtgcctcg cgttccagca gactgtcggg aatacctgcc
     6421 agccggagcg cattggcatt gcgaaaagca tcgagcaata cgatggcctt gcttttttcc
     6481 gagagttcga atgcagcggc gtgaaacttc gggcccagtt cccatgcaat ctgtatgccg
     6541 tgctcaaaaa tttgtgcgct ctggcgaaag aaaagcagct tatcgtcttc actttgcaaa
     6601 gtaccggttt tgagaatatc tacagcctga acagcagctt gtacggtggc ccaggcgctg
     6661 agtaaatact cgtcattttc actgacggcg gcccaacggc gcagcgtttc tattttgcgc
     6721 cccaaaacgc tcacgatgtc tttgtgtatg aaggtacttt gaaaacgaac cggattttgc
     6781 cggatgtcgg tttcatcaaa gccctcggta agactgatga gcgccgattg atagtgttcg
     6841 agggcgcagg catagtcgtt ctggcgggca gccagttcgc catacgcgga aaacacttcg
     6901 gctattttgt agtgtttgag accgaagacc tggaaacggc ggtcgaaggc tcggttgaaa
     6961 taatggtggg cctgggcgag gttgccttgt gcagcgtaaa gttcgccgaa gtggaaccag
     7021 agatcaaaat caagccctct gctgttgcct tccgccattt ctgcctcccg cagggcgccg
     7081 acggctgcgg aaaacttacc attggttttg taaaggtggt ggtcagtcca ttgcgatttc
     7141 ggcgttgggt ggtgtcggtt tgggtataaa tctgttttgc cttaaggtaa taatattccg
     7201 atttttcaaa atctcctcgc tgaaaaaaca gatctccgag gtgcttgttg ggcagggcga
     7261 tatagccgga actgcgctcg tcttcgccga cggctttttc gtagtcaata cttttgagat
     7321 aatattggta tgcgatatcg tactccgccc atctccggtg aatggcgccg aggtatttac
     7381 aggtagaagc taaatgttca ttggtggttt gtgacggtgg agactgcgcc tgaagatcnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg
     7561 tgtttctttt tttcaggaat tactttttgt gccgttttga gtgtttcata aaaaatatcc
     7621 agacgatccc actgacgggc atactcaatc tgaacactta agatatccac atacattttc
     7681 cagctctggg catcttccaa ttttcgggtg agctttttga gatgatcaaa gatgcacggg
     7741 ctatcgctat tggcctcaca cgcgggaatg agttcctcca gtactttctt atgcacggag
     7801 gtcgtcatct gtttgagccg aaatcgaagt aatgagaaac aacattccgc cccaaagcag
     7861 tattcttctt ttcatcagtg tgttgtttat tggccaaaaa cggaaagcat tcctgacgct
     7921 caatgcccca agaatgcctc tgtcaaaata ccttatagcg taatcagctg ccctttagaa
     7981 acttgcaaaa gtactccttt gtcgtccacc ttcttgaaag tctatccggt aaagctgtcc
     8041 ggcagccaag ttttcaggaa gggtaatgat tctgataccg ctgttccctt catacttttc
     8101 tttttcatta cccggcaaac tctcaaaaac aagattgttt tgtgcgtcgt aaatgctgac
     8161 ctttagttgc aattcgaacc ggcatgccca tcttttaaac agagataaat catagcattt
     8221 gcccttgcca caaggttcgc cgattctcga caaagcacgg ggcgggcaat tggggatgcc
     8281 gttacataat tgttcattga tgtatgtagc attggtcctt tcatacttca tttgttcttc
     8341 caagacctct cgttctttcg aaatttcgat ttcggtgatg ggtacatccg tttcaatggc
     8401 gtcgttttca gtgccgggtg ttgtttcgag gctatctaat tctgcctgaa tatcatccgc
     8461 caatagttga tgaatgctga gcgtagcatt catatctaat ggaggaggct cacaggcaaa
     8521 caaaaagacc gccaatacta cggtcacggc tacaggaaat aaaagggtgg ttctcatgtg
     8581 aatggtttaa gatgtgtaca aaattgattc tatttctgct aaatcgaaaa tccgatccaa
     8641 ttgttacccc gccaaatagc ggagtgggct ttttaattgt ctctctattt ttttcgcttc
     8701 ggcgtaataa gggtgcgatg catcgcccaa tatttgttga agatatgcct gcgccggttc
     8761 gcttttcggg ttttcggcaa gtgcagccaa cagcctgtgc cattctgcgt tgtgcaaaaa
     8821 acgtgcatcg ctacttttta ttaccagatc aaacgctgcg agtgctgccc catattgccc
     8881 cgactcatag caggcatacc ccttgaagag catggcctcg gtatattgcg ggtcatcggc
     8941 aggcgctgca ttaaatgctt gaatggcaac tgcatacttt tggtttcgat aggcggtata
     9001 ggcttcttgt aaatcgtaag tttcacccga taaggtggcc ggagtggccg ccggggcgta
     9061 gtgctgtgcc aaaatagctg atatttcgta ttttgcagtg gcataccatt tgagacctgc
     9121 gaaaacgagc gccagaacag ccgccgccgc agccaaccat tttcgccata cgattcgagg
     9181 ggctgcttta gagatcgttc cggcaggctg ggattcgaca gggaaatagt gcgccgagtt
     9241 ttttttcaat attttttcaa aatcggctac ctcgctatcc gcaacagcac ctaatgcagc
     9301 ccggccctgt cgttgcatag acaactcggc ctgcaatatt ttatccttac gcaattgagc
     9361 ctcaaatgtg ctcaactcat ctccggccaa ggttccgtct aagtattttt ccattaatgt
     9421 gtaagtatct gtcatagctt attggtcttt gagttgtgta aattcgggag catttttcat
     9481 tttctctcgc aatgcctgtt cgcactctga tttctttttg cgcaaataag catagctcaa
     9541 tcccaactgt tctgcgactt ccgatagagc gggatactta ttggtttggg aatgaggcgt
     9601 ccatgccaat ttcaaaatat cgcgacagga aggccccatg gtttcaacgg tggctgccag
     9661 cagagccaac cgctcctcgt gctgatctaa acgatctgcg ttgtcctcgg ctatttcggt
     9721 agtaaatcgt tccgaacctt ttattgttac ttccattcgc ccgcgtcgcc gaagctcgtc
     9781 cagccatttg ttgcggcaaa ccgtgtataa aaaggatttg aaagaacagg tgagtacata
     9841 tcctctttgt gcctgacgat aaatatctat gagcgcatct tgaaaaacat cgccggcatc
     9901 attcgttgca cccccatttt ccgtaactaa ggcccgtatc atatcggaat aatccctata
     9961 gacagccgca acaacgctgg aatcatgttg taccagcgct tcaagaatgt attggtcgtc
    10021 gtgaggaatg tgttgagtac tcataggtgt aagcaggtgt tagaacctct gcaaaaatat
    10081 ttttttttct gcaacccaaa tgcaaatggg gtaacaattt cagaaagaag cagatagagg
    10141 ggtattcaat caattttttc aaccttaaaa acgcatttta tgaaaacgat tcaaaaaatg
    10201 atcctcatga ccggagtggt cctgttggtt ctggcaggca tcagtatgtc ggggtgcaaa
    10261 ccagacccca ttgaacccgc caaaatccta tccctcgaca tcacacttcg caccggcggc
    10321 gatgatctcc ggcaggggag tgaagcactg ggtacgattg gcttcaaaaa tggctccaac
    10381 acgagcatca gcctgaacaa cggggccaac tggggcaaca attctaccaa ttccgtcagt
    10441 atgaccatgc cgcccaacac ggaggtcggg agcctcaact ggtttaaagt caatttcact
    10501 tccggtagtg gcggctttac cggcgacaac tggaacctgg accaaattaa gatcacggcc
    10561 cggctctccg atggcagcaa tcttattttg gcggaaagaa caggctcgcc attggtgagg
    10621 ttcactcaga acgaggttcg agagtacact gtaagttttt gattatcaat tgcgttctgg
    10681 gacgcatata aatgatctgt atgtggcggc gccttcaata gattgggctg ctgcgtacag
    10741 atcagactta ttgaaacggg ccggggcaga taaaaaacga agctgttttc agtccaaaca
    10801 gagattttta aaaagcacat aacccctaaa aggcgccgaa tgaattgaaa acagccgctt
    10861 tcacccgatg atgattccgt cgaaacaagt ctaaaaatag ccatgcactc cgtcggtgaa
    10921 aaaacagaat tgaaaataaa gtttacaact tgctccttgc ccctcctcat ctgctctcca
    10981 gtattaacaa actgttaaac ccctcgaata aaaccatcgc tcccggcgca ccatccgtct
    11041 gatattggtg ttacatttgt tcacctttaa aaccactgtt atgatgaagt cccttttatt
    11101 ttcggcactt ttcggtttgt tgttcctttc cttgtcggca caacccacaa agggcgcctg
    11161 gatgttgggc ggcgatgcgc gttttcagtc caatatcggc ggaaactcat tcaatgcact
    11221 gagcctcagt ccgcgagtcg ggtattttgt ttccgaccga ttggcgctgg gcatgggcct
    11281 gggtttggcc gtgctcaaca cagagtttgc caattatgac gggggaaacg tctctccttt
    11341 tgcccggtac tattttccgc tcaaaaaatc aaagctgtcc tttttgctcg aaaccggcgg
    11401 ctcattcagt accatcacca taggcggcga aacaaccaat acctggcgcg ttcaggccgg
    11461 cggcggcatc aactactttc ttacgcccga attcgcgctg gagggcatgt tgggcgccgg
    11521 gcgggtattc ccaacggcag gcgaccattt cacagaaacc gattttcgat tgggttttca
    11581 ggtatatctg caccgaaaaa gcagtgatga cgatgcgccg atcggttccg gggcgctgcg
    11641 caaacggagc ctcttacttg gcgtagcccg taccctgacg ctcaactccg acaatcaacg
    11701 ttttgagcct gctttctcat acatgctgag cgaccgcctc gctgtgggcg ctgctatcac
    11761 cttgcctatc aatgaatttc gaccccacct gcggtacttc acgggcagcg aaaatgcaag
    11821 ccggctgagg ccgtttttgg gcgcaggcag cagctttatc tgggaatatg gagaaagcga
    11881 ccagttcaat atcacgccct tcgccgacgc gggtgtgttg tatttcatca gtcccaacat
    11941 tgccttagag ggcgctttgc aatatcaggc tgatttcagg gctttccgcc cggcttccga
    12001 cctgacttca aacctcatgg cgcgggtggg attccagtat gtgctccccg gcaagcgctg
    12061 atacccgcaa ccacccggct cttcctgctg tttcacccgc atggacacga tcacacccac
    12121 accgcaggcc ggctggcatt tcgataacag ctacgccgcc ctgcccgata tattccgcgc
    12181 cgaactgccg cccatgcccg tgcaggcgcc ccggctgctg ctgttcaatc gcacactggc
    12241 gctcgaattg ggcctgcaac cggaagtgtt ggaatccgat tacggcgcgg cggtattcgg
    12301 cggcaaccaa ttgcctcagg gcgcccggcc catcgcgcag gcgtatgcag gccatcagtt
    12361 tggtcatttc gccatgctcg gcgacggccg cgccatctta ctcggcgagc agatcacgcc
    12421 cgacggcaag cgcttcgaca tacaactcaa aggctccggc cgcaccccct tttcccgctc
    12481 cggcgatggt agggcggcac tcggccccat gctccgcgag tacatcatca gcgaggccat
    12541 gcacgcgctc ggcattccca ccacccgcag cctggccgtc accctcagcg gagaggccgt
    12601 ttaccgcgaa accgccatgc ccggcgccat tctcacccgc gttgctgcca gtcatatccg
    12661 cgtaggcacc tttgaatatg tggctgcccg tcgcgaaccg gaatcgctgc gcaccttagc
    12721 cgattatgtc attcgcaggc actatccgct tatggccgac gccgaaaatc cgtatctcgc
    12781 tttgctgcgg gccttcattg acagacaggc agctttgacg gcatcctggc tggccgtcgg
    12841 ttttatacat ggcgtgatga acaccgacaa catggcgctt tccggcgaga cgattgacta
    12901 cggcccctgc gcatttatgg acgattacca tcccgccaaa gtgttcagtt ccatagacac
    12961 gcagggccgc tatgcgtttg gcaatcaacc caatgtcgcg cagtggaatt tagtgcgttt
    13021 tgcggaaacg ctgatcccgc tcctgcacgc cgaagaagca caggccgtcg cgctggcgga
    13081 agaggccatt gagccgttcc cgcgcttgtt ccgccaccat tggatgctga tgatgcgtgc
    13141 aaaaatgggg ttctacaacc gggagcccga tgatgtggat ttgctgaaag aactgctcga
    13201 atggatggag cagcaacacg ccgattacac caacactttc cgcgatctga gtcgcgccga
    13261 actgcccgaa ctgctggcct ataaagaggc ggctttcgcc caatggcaca aaaaatggct
    13321 ggctcgggtg gcccgtcagc ccgaaccgat ggaggacatc cgccggcgaa tgcggcagac
    13381 caatcccgcg atcataccgc gcaatcacaa agtagaggaa gccctgacgg ctgcggcaca
    13441 gcggggcgat ctggggccgg tgcagcaatt actggctgca ttggcgcgcc catacgagga
    13501 gttgcccggc ttggaagcat atcgcctgcc gcccgcgccg gaggaggtag tggagcagac
    13561 tttttgtggg acttaaggaa cgtgtcgtga ataacttacc gatttggtcg gcctcttttt
    13621 ccaccaggct gcgttgcttc gtcagtcagg tagcgctgct atcctccatc ctcgcgcctt
    13681 gcctgatgaa aaaatagtct cgccgaaacc aggcaactta ttcacgccac gttcctaagg
    13741 gattcctcag gccacccgtg ccgatttccc tccctgcacc tcataaaaat acccgggatc
    13801 attgaggatg tcgtagaggt actcgtggta ctcggcaaat tccggcaggt cattgtgtcg
    13861 cccgttgggt atggtgagca gtgcgcagcg gtcggggcaa agtttttgca acatcacgct
    13921 ctgttcgtaa ctgatgaggc ggtctttgtt gccatgcaga ataaaaaccg ggcaggtgat
    13981 tttgcgcata aactggtcgg tgcgcaactg aaaacgcaac aacctttgga gcggcatcca
    14041 aaaaccatac cggcgaatct gatataaaaa actgtaatat ggcgagtcga gaatgagcat
    14101 ctggggcttg ttccaggagg cgatgcgcgc cgcaatgccg ctgcccagag agcgcccgta
    14161 aatgatgatg cggtcttccg ggaactgggt gctgagccat ttgtaaaccg tttgcgcgtc
    14221 gttgtagagg gtggtttcgg tgcgccgccc ggagcttttg ccgaagcccc ggtagtcgaa
    14281 cataaaaaaa tcgtagccct tgctcacaaa atcgcgggca aacttgcccc agcccttgat
    14341 gctgcgcgag ttgcccttga ggtaaaacac cacgcctttg gaattgggca ctttgaaatg
    14401 caccccgttg acagcgccgc cgtcttccat ctcaaaattt acttctgtga acggaaacgg
    14461 gtatttatac tcaaaatgct gcggcagtat ctccggccgg aagaagaagt agtcttgcag
    14521 gaaatagaat actgcacaag cgccgccgta aatggccgcc gccaacacaa tgaaagacgg
    14581 gatgacgatc atggccgatt acttttccaa ttgctcgatg aggcgggtag agatgcnnnn
    14641 nnnnnnnnnn tttcggtgat gatgccgatg agttcgtcgt tttgcaccac cggcaggcag
    14701 ccaatgcgtt tttcgcgcat cagattcatg gcctccagca gcgtagcgtt cgggctgatg
    14761 gtgatgggtg cttctatcat gatgtctttc accatagtag cctccttgct gttgagcttg
    14821 tttttgcgcg caaaatgccg cagcagcagg cgcgaagtga tgaggccgca gagctgccct
    14881 ttggattctt ccaccggcat gtagcggatt tttcgccagt ccatcaggtc ggtcaccaac
    14941 tctatgaggt cgtccttttg aacggtaaac aggtcggttt ccataaattc ttccacgcgc
    15001 aacttagacg ggtggtagtt ggggaggtct gaaaatgcag gcatggtcca ttggtgtacc
    15061 ggaatatcct tccattggtt gtgaacaata gcggccgtca tcgcggtgac ggcttcgtcg
    15121 cgggtgcatt ctttggctag tttggtaaaa gtgcgcaatt gccagcgggc gccgttcatg
    15181 tgatcgcgtg cgcgttgctc tatgatgccg aggtatttgt caatatcctg tttgatgacg
    15241 ccccgcattt gcagtccctt gcgcgccatg ggcagcagtt cgtgcaaaac gagatcgcag
    15301 gcagagagtt ttttgtcgtg gaaccagtta aagtgagagt caatgccgaa ttgggcggct
    15361 ttcataaaat tgtcgcgtac gtcttcccag gaaacatggt tgcgaatgtc tttgtactcg
    15421 tggcccattc cgatcatagc gcccaaccag aacgcggcgt tggcggcggc atctgtggtg
    15481 gtggggcctg ccggaatcac gcggttttcg atgcgaagat gcggctgccc gttggggctg
    15541 atgccgtagc aggggcggtt ccagcgataa acggtggagt tgtgtacctg caaagcgcgc
    15601 agtttcgggg tgcgtcccgc ttgtatggct tcgatggaat cttcgtccac atcgccggcc
    15661 agcaacacgc ggaaacgagc gatgtcttcc ttataaattt cgagtaaact gccttcgagc
    15721 cagccggtgc cgaaactcac gcgggggctg cgctcgcgca tgtgatcgtg ggtggtgcgc
    15781 acgtccaaag cctgctggaa caaggcaatg cggctctcgt gccacagtcg tttgccgaac
    15841 acaatgggcg agttggccga tatggccatc acgggagcgg ccaatgcctg cgccatgttg
    15901 tacataggta caaaaagatc gggcgatacc tgcaaatgca cctgaaaact ggtgttgcag
    15961 gcttcgagca gcggtgaatc gtgtttcacc aagagctcgt ctatgcccga cagccgcaac
    16021 tcaaacgccg agccgagcat ctggctgttg agggcctcca tgagcgcgaa atagcgcggc
    16081 ttgggcgtga gattgctcag gtccaaatcg ggcttgcgca gggtgggcag gatgccggtg
    16141 aggcaaacct cggcgttcca gtgtttgagt tgctggcgaa tgatgttgag gtattcgcgg
    16201 ttttcatggt ccatttcccg caggcacttg ccggtaaact ccctcggact gagattgacc
    16261 tccagattga atttggccag ttcggtcacc acccagggat attgcgatag tttttccagc
    16321 acctccatgt tgatgcaggc cggcttgtag gtgccggcat ccacaaggca catttcctgt
    16381 tcggcgccta tacgaatgat gtctttttca aaccagtcgt tctccagcat gtactccaat
    16441 gcctgcacat cgcgcagcaa actgcgcaca aatgactgca tgccggttcc ttcctgagcc
    16501 actgatactt tttgctctcc catcctattg ttgatttttg catcgaaaaa taggcagata
    16561 ttgcaacact caaaatgttt ctgaaaaaaa attcagaatt tggcaaaaaa cataccgctt
    16621 cgccctgcgt tcttcatgcg gtgagcaatg aaactcgaaa cccgcgactc gaaacctgaa
    16681 actcgaaacg cggaactcat cgctacccga agcatcctct ttttatccat ccatttccaa
    16741 tgagagcagt actttatcta tccattttcg gcctgctggt atcggcttgc cgccaagaca
    16801 acggcgagcg cgttttcgag atggtgtttc ccaacatcac ttttgaaata cccgccgggc
    16861 tgaacgccac ctttcaacaa gtttttcaaa ttccgctggt gcctaccaac atagatacct
    16921 atctggccga gaacggcacc gacaccgcgc tcatcacggc cattgcgcca tttaccgcta
    16981 cgcttacctc attggagggc gcggcatttg attacgactt cgtggaaggc atttccattc
    17041 gcatctgcga cccggtcaaa ccggcttgct ttgatggcga cgaggttttt tatattgata
    17101 atttgcaggg aaggggcggc caaaccatcc ggctcattcc gggtttgcgc aatgccaaaa
    17161 gagacctcgt ccggaggcat tttcgcatgg aggccgtgtt tttctttgct tatacgacgc
    17221 cttattccgt caggtcgaga ctgcaaatga acttcgaagc gctgaagtag tgtgcaggct
    17281 cacactttcc aaaacttcga caggtgcgat atggccatgt tggcccggat ttcgtactcc
    17341 tcccataccc gaagaccgga agacgtttcg ggttcgggat agacgatggc ttcgccgttg
    17401 atgcccgcca caaagttata agcgctgttg cgcaaccact ccagattgta gccgccttcg
    17461 agggtggcaa acatgcgcgg gaatttggcc gccagcagct ttccgagttt gtaatacccc
    17521 gtagccgaga cattgagctg aagcaagagg tcgtactggt gcgcatcgaa gcctgccgag
    17581 agtgctacca catcgggctg gaacagctct gcaaccggca gaaagcggtt gacggcatcc
    17641 agaaaaatat cgtctgccga gccgggcggc aggggcacat tcaccgtgta gcctnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnattgatg aatagaccag tacatgacct gatcgtcgtt gtaaaacatg tcggaagtgc
    17821 cgtcgccgag gtgcccgtcg aagtcgaaga tgagtacgcg tttgccttct gagcggagtt
    17881 tggcggcagc gatggctacg ttgttgaaca ggcaaaaacc gctggctctg ttggggtaag
    17941 cgtggtgccc gggcgggcgc acgagggcga aatcgccggt ttcggaagcc gctatggtag
    18001 cgccgacggc atgaatggcc gcctgaaagc tgccgggcga ggtcacggta tcggcatcca
    18061 aatggccgcc gagatcgcag gcatcgcgca ctttttcgat gtagcgcggc gtgtgtacga
    18121 gatggaggta ttcggagccg tccactaagg gcgtttcggg cagattggta aacatttgca
    18181 gacgcctcat gctctccggg tgcatcccgg tatcgtggtc gaggtagatc gggttgtaga
    18241 gaaatttcat ggttttattt gttgatcgta tcaattttat tcagacagct acgagccgcg
    18301 agttgcgcgt gagggggcgc aggggagtga ccttaggaac gaccgaagca gccgacggta
    18361 cgccgcggcg tacccggcac gcccgcttcc cctctcaacg tctcaaaacc ccgttccctt
    18421 caggcccagc cccgcggttt cctgccagcc catcatcagg ttcaggtttt ggacggcctg
    18481 gccggaagcg cccttgagga ggttgtctat ggccgaatgt acgacgagct ttccgtcgtg
    18541 cagttctgtg tgtatcaggc atttattggt attgactacc tgcttgaggt caatcatcgc
    18601 gtcgctgacg tgcgtgaagg gcgcatcggc gtaaaaattc cggtacaggg cctgtatttc
    18661 ctgcaagggc cggtcggtgc tgatgatgga actgatataa atgccccgcg taaaatcgcc
    18721 ccgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnagaacccg tgatgccggt gctgtatgtt tcgcccaaaa tgccggcttt ggccaatggc
    18961 agcaggccga gttggatggc tgtggcaaag cagccgggat tggcaatgct tcgggcctgg
    19021 cgtatggcct cgcggtggag ttcgggcagg ccgtaaacga agctgcggct gccgatgcgg
    19081 gcctgttttt ccaatctgaa atcctgcgag aggtctatga tgcgagtgga gtcggggatg
    19141 tggttcgatt ccaggaattt gcgcgcctcg tggtgaccgg agcaaaggaa gagtacttcc
    19201 gctgctgtat caatggcgtt ggaaaaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacagc gtgataaatg gcagtttggt
    19441 tgccgaaaat cttgctgaat ccgcgcacat cctcgccggt ccagccactg ttcatctcgc
    19501 cgtagctgcc gaaacggctg ctcatcagat cgtgcggcga ttcgatgccg ttgatctgga
    19561 agcggtaagg gaagagttgt acgaacacat cgccgctcac tttttgctgc gagttttcga
    19621 gcaagg
//
LOCUS       DAORLF010000030        19291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1518_length_19291_cov_2.007902, whole genome shotgun sequence.
ACCESSION   DAORLF010000030 DAORLF010000000
VERSION     DAORLF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_19291_cov_2.007902"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..900)
                     /locus_tag="PK198_04455"
     CDS             complement(7..900)
                     /locus_tag="PK198_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38020.1"
                     /translation="MRSQPSIFLVSIGSKAMLLFLVIILPLLMKAQGRQGGINFTKEL
                     FDLKNENIDLNISQRQILSGWRLDGGSLASEVVAIKDIRDAVSNNRISVSLPGDTSKY
                     TFVVKDFEQEAPDNYTWYGELKESYGYLFLLRRPEGFIGGIQTPTKYYSIAPLGKEFA
                     LVMLKDHSETAVADCGMDGIEAAQMSTPEICDEGFNTCPAVIDVLSIVTDNALAFLQN
                     RFPAVTFDVPPLGQVQLALWPLALEWAYANTNRALQNSDVPNKRFRFRTQIQNVPLTN
                     DIDVDVDTMVREESPLCGVGL"
     gene            1478..2287
                     /locus_tag="PK198_04460"
     CDS             1478..2287
                     /locus_tag="PK198_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38021.1"
                     /translation="MKKILLVAVAVFCTQLAYSQADTPRVKSLPQEAKGIVYNKEFTV
                     DFKMHTSGFGLGANWGKLKTYYRTRFFNLELAEIKHPKEIRQSFDFRIPSSGRISRSF
                     IFGKQNNLYVLHGGIGEKRYFSEKAINRGLAVGISYSGGPTLGLLKPYHLDLIRFQEQ
                     DPTKFFISAESFSEENADKFLDLNSIYGASGFSRGFDQISISPGAHAKFAIHLDWGAY
                     DEFVKSIEAGVMVDVFFQTIPIMVESPLVPDTGNQSVFFNLFVNLQLGKRR"
     gene            2296..3168
                     /gene="lipA"
                     /locus_tag="PK198_04465"
     CDS             2296..3168
                     /gene="lipA"
                     /locus_tag="PK198_04465"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRF38022.1"
                     /translation="MIELNIVESKEERQRKPDWLRVKLPIGQNYRHVRQLVDQYSLHT
                     ICQSGNCPNMGECWGAGTATFMILGNTCTRSCSFCAVKTGRPNEYDEDEPRRVSEAIR
                     LMGVKHAVITSVNRDELADRGAEIWYQTVRAIKESTPQVTIETLIPDVKSTWSALERM
                     ISAGQEVVSHNMETVQRLYRKVRPQAKYERSLEQIRRTKEYGKRTKSGIMVGLGETEE
                     EVFQIMDDLLEYGCDVLTIGQYLQPTKMHIEVAEFIHPDQFARYTEVGLAKGFPFVES
                     GPLVRSSYHAERHL"
     gene            3181..>3624
                     /locus_tag="PK198_04470"
     CDS             3181..>3624
                     /locus_tag="PK198_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRF38023.1"
                     /translation="MNQSLRIARIFGIPVQVHWSFGLLLAWVAYMGYEQGWTLRETLF
                     MGGFVLVLFICVILHEFGHALTARRYGVGTRDITISPIGGIARLDRMPEKPYQEFMVA
                     IAGPLVNVAISGILSVVYLVWSGAGSQALQHSLYGIILRDSNFLPG"
     assembly_gap    3625..3722
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3723..4310
                     /locus_tag="PK198_04475"
     CDS             <3723..4310
                     /locus_tag="PK198_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38024.1"
                     /translation="GRVLRALLSVRWGRSTATRIAAGLGQLLAVGLAIYGAYTGSFVT
                     ILIGGFVFLAAAHEHRAVRAEEFLSHYKVSDVVRTQFSRFFPFDPVSLPAEAMQHGLE
                     QYVLVFDPAYQVVGSLSETVVLMAVKEELYDAPIESLPLLPAPEVRPEQTLKEVLHIF
                     QTETAPILPVVENGALIGVVDQPMVEHFLRRQRKR"
     assembly_gap    4370..4379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4380..5295)
                     /locus_tag="PK198_04480"
     CDS             complement(<4380..5295)
                     /locus_tag="PK198_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF38025.1"
                     /translation="MSQPNSVRTHDALFKHLFSDLGRVREYFEVYLPIELCSRVDWDS
                     LQREDGTFVDDTLKASYTDLVFSVRLKDQTEVYLCLLLEHKSHPDKNTPFQLLYYISG
                     SLWREAQNGQTPRLVVPVVFYHGEEPWEYKPIVKHFGHLPEDILCYLSKFEYAFHNLQ
                     KNPDEQILAAFRNHLLISALLTLKHSFDEAYLQNQAVFLLESAVDDDGNYFKALFVYF
                     FNRIKNEENMTKVLEQLSNEVQAEAMSVLDLYEKRGIQKGEQIGIRHKSYLACRNMIL
                     KGFDDKVISEVLEVEESFVQQVRAEINNM"
     gene            complement(5375..6424)
                     /gene="ilvC"
                     /locus_tag="PK198_04485"
     CDS             complement(5375..6424)
                     /gene="ilvC"
                     /locus_tag="PK198_04485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRF38026.1"
                     /translation="MAKLNFGGVEENVVTREEFPLEKAREILKDEVIAIIGYGVQGPG
                     QALNLRDNGFKVIIGQRKDSATWDKAVRDGWTPGETLFDIEEAAQKATIIQYLLSDAA
                     QIAVWPVLKPHLTAGKALYFSHGFGVTYSERTGIVPPADVDVILVAPKGSGTSLRRLF
                     LEGRGLNSSYAIAQDATGRAFDRVIALGIGIGSGYLFETTFKREVYSDLTGERGTLMG
                     AIQGIFAAQYECLRRNGHTPSEAFNETVEELTQSLMPLVAENGMDWMYANCSTTAQRG
                     ALDWYGRFRDAVAPVFEDLYNSVASGEEAQRSIDSNSQPDYRVKLEEELRELRESEMW
                     QAGRTVRSLRPEAGK"
     gene            complement(6448..6984)
                     /gene="ilvN"
                     /locus_tag="PK198_04490"
     CDS             complement(6448..6984)
                     /gene="ilvN"
                     /locus_tag="PK198_04490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRF38027.1"
                     /translation="MEKEFTITVFSENQTGLLQRIVAVFTRRHINIESLTVSKSSIPG
                     IHRFTIVVNVTEDMVHKLVAQLDKQVDVLKAFHYERDGIVFQEIALYKVPTEAFSNGD
                     TVEQLIRAHNARILCIEPEYVVIEKTGHASETEALLQDLEKIGIYEFVRSGRIAIVKA
                     MERLNTYLKDMNIQINHR"
     gene            complement(7142..7318)
                     /locus_tag="PK198_04495"
     CDS             complement(7142..7318)
                     /locus_tag="PK198_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HRF38028.1"
                     /translation="MAKVSKNARVAQRSANAGSGKDHVRVVKPVKDPKTGKYTYKEVI
                     VHKDKVKEFFSDVK"
     gene            complement(7394..7582)
                     /gene="rpmG"
                     /locus_tag="PK198_04500"
     CDS             complement(7394..7582)
                     /gene="rpmG"
                     /locus_tag="PK198_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRF38029.1"
                     /translation="MAKKQKGNRIMIIMECTEHKASGMAGTSRYITQKNRKNTPDRLE
                     LVKYNPILKKKTVHREIK"
     gene            complement(7611..7862)
                     /gene="rpmB"
                     /locus_tag="PK198_04505"
     CDS             complement(7611..7862)
                     /gene="rpmB"
                     /locus_tag="PK198_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRF38030.1"
                     /translation="MSKVCQLSGKRPVSGNSVSHSNRKTRRRFLPNIQTKRFYIPETD
                     EWISLKVCTSEVRTINKLGLYAYLKKLEKKGVDTGVKLK"
     gene            complement(7906..7979)
                     /locus_tag="PK198_04510"
     tRNA            complement(7906..7979)
                     /locus_tag="PK198_04510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7943..7945),aa:Arg,seq:acg)
     gene            complement(8158..>8436)
                     /locus_tag="PK198_04515"
     CDS             complement(8158..>8436)
                     /locus_tag="PK198_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38031.1"
                     /translation="TNGRLLTRAKLLLIDVKVLDTPINLDAVNYERLEVGVVAGAGVN
                     INLGGTKLFFDGRYSHSFTQVYDIPVIEDRVSNRGFSINAGLMFPLGR"
     assembly_gap    8437..8686
                     /estimated_length=250
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8687..8868)
                     /locus_tag="PK198_04520"
     CDS             complement(<8687..8868)
                     /locus_tag="PK198_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38032.1"
                     /translation="MKTTLQLAAFSIAFCLGITLSAQVSLGFRTGATWSNVQASEGLD
                     ALAPDFKTISGFNVAAV"
     gene            9036..10886
                     /locus_tag="PK198_04525"
     CDS             9036..10886
                     /locus_tag="PK198_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF38033.1"
                     /translation="MHDLPKERKTFFFTDMEGSTRIAQRLGEGYAAVLERYRSIIRSA
                     VGHHHGQEVDTAGDGFFAVFNHPSDAVAVAVAVQRAFSVENRTGSDNYHVRIGIHNGE
                     AVRSPDGGFIGIEVHRASRVCSAAHGGQVLLSEASAQAVQPKLPTGTELAQLGAYLLK
                     DFEKAENLYQLVIPGAPSEFPPPRTSIQTHTIAVLPFRNTGADKKWDNFCDGMAEEII
                     VVLGKVQGLHVVSRSSVFALKNREEMDLQETARQLHATVLLTGAVRFSGRRLRVTAEL
                     VDVESGLNLWSGRYDRLMKDVFNVQDEIAANIASALRVKLVSRQVRGISSMQTTNINA
                     YDYYLRGRRFYEQFSRQSVEFAVQMFRKAVEEDDNYALAYCGLADCYSYLYLYLEDSR
                     EHLDAAQQYSMRALELDPLLAKVHVSRGIALSLDKHLVEAEASFDRAIDLDPRLYEAW
                     YWFGRICFTEGKLEKAAAMFEKANRLSPTDYQAALLAGQVYSDLGIMEKSEAARRLGV
                     EMAEQALELNPGDTRALYLGANGWAALQESDKAMLWLQRALTLEPDDAMLQYNAACVY
                     ALLGKNDEALTCLEHSVDLGLSQKGWFENDSNLAPLRQEARFRALYERLQ"
     gene            10993..11067
                     /locus_tag="PK198_04530"
     tRNA            10993..11067
                     /locus_tag="PK198_04530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11026..11028,aa:Arg,seq:ccg)
     gene            11167..11240
                     /locus_tag="PK198_04535"
     tRNA            11167..11240
                     /locus_tag="PK198_04535"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11201..11203,aa:Ile,seq:cat)
     gene            complement(11381..11605)
                     /locus_tag="PK198_04540"
     CDS             complement(11381..11605)
                     /locus_tag="PK198_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38034.1"
                     /translation="MQDSIENCLYVTAQGELLQLFCQYQVICINDVHKLKKGDLLWVE
                     AVLMSRDGQVVYQIEGEYYYHSHFAVYQLS"
     gene            complement(11690..12067)
                     /locus_tag="PK198_04545"
     CDS             complement(11690..12067)
                     /locus_tag="PK198_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38035.1"
                     /translation="MITIVGYAPRQNSAGETFYTLLLQGGIEMVKSQTTGNYYATARR
                     TSVTSTFDERTCKALIGEKLQGHVARMECESFDYTIQETGEVIKLNHRWVYVPEGQNE
                     QEVIRAERQAQSNGMLVGAAVIH"
     gene            complement(12169..>12970)
                     /locus_tag="PK198_04550"
     CDS             complement(12169..>12970)
                     /locus_tag="PK198_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38036.1"
                     /translation="NCRALMAEPLDTLDPQGDWIIHYIKDFSLPRDKNIIILSATAHE
                     GFYRTLYGDRVDVVDISDIECKANLIQISDGTFSAHKLKENPALLLSINDFLPDLPAI
                     TFKKLKTQVKGAHPALHFGNAAGDNSLKGKDLKIVGTPRMDATHYILIAKALGHAISP
                     NAHTVAYQKVQCNGYLSYQTVSYDPVIRDIQLYFTGAELTQAIGRVRPYEHASDVYIF
                     SEYPVPGAVQYSMKDLPLLAQCRTSNKNAQTEAGFHAENTVKREILKS"
     assembly_gap    12971..13130
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13353..13469
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13470..14903)
                     /locus_tag="PK198_04555"
     CDS             complement(<13470..14903)
                     /locus_tag="PK198_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38037.1"
                     /translation="MPYDNIKSQQAPADCANSEPQAHRLVKLTLSPEDRKNKPQDMVR
                     ENGLIHKSNACELTLPELVRMLTAPHSRTWCCSVLSGTRKESNFESTELLALDFDKGW
                     TPEQVLETAREHGLPPLLIYSSFSDDPHGERRFRAVWLLDQPIFDKKVAKECAHNLSV
                     IFPGCDPRSKDVVSMWFGGKEVLYAAWKDAGRGHGGQIALPLITAIALEEAGLPYRLS
                     EVDAKKWSRTFQRHTLTGPTTEKNKNGGKTGSSNDILIRSGQIPPDFVEKEASQEGIT
                     LDYIEIFDWDEAAQVAQLLSDLIAARRKILHPELFWLAGNMAFIAGGARLFKECIDRN
                     PLINQDKKCLMDYVRYRFSTLNPIYPPGFDSLPADHPDHALYLHYRNLLHVRITKSQK
                     AMYINDSRNLIPLATARQELHTKVKEVLSRNHHKITLFKVGVGVGKSHLVSGLDLPNA
                     LYLLPTHKLIEQASKASKADHLCTPAIP"
     gene            complement(15005..15403)
                     /locus_tag="PK198_04560"
     CDS             complement(15005..15403)
                     /locus_tag="PK198_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38038.1"
                     /translation="MICIKDETLERLLQEERGNTLAMLLAIQRMRGLKEHITVRTLKG
                     QAGIKSTYLNRYLQRLEKAGYLQRVRTCADPDFGHIDYRLNCEGLSYVPDDAAPESSG
                     ADDKATLFLLLQMIHRLIAAITGTLEPRKS"
     gene            complement(15744..15959)
                     /locus_tag="PK198_04565"
     CDS             complement(15744..15959)
                     /locus_tag="PK198_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38039.1"
                     /translation="MVFTGRFGAYKSLVGTEKNAVHLSSKWPFKAAIVTETAGMSAFD
                     QRVKLGGCGRYQAANFANLPSGEFGRL"
     gene            16579..>16776
                     /locus_tag="PK198_04570"
     CDS             16579..>16776
                     /locus_tag="PK198_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38040.1"
                     /translation="MLGLFKRTNSIQSEILSDLQSAKKSIKVAVSWLTDTSLIQELIK
                     KRRQGLDVKILVSANELNVIRF"
     assembly_gap    16777..16795
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16796..>17089
                     /locus_tag="PK198_04575"
     CDS             <16796..>17089
                     /locus_tag="PK198_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF38041.1"
                     /translation="QHLGGFVQKWGNEETSEGGFMHFKFYIIDDSFAKSGSYNWSINA
                     TSNAEALDKVDVFKKIKEFQAYFSESVDFFAAISNPERKRDELELIRKEKHQDV"
     assembly_gap    17090..17176
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17177..17373
                     /locus_tag="PK198_04580"
     CDS             <17177..17373
                     /locus_tag="PK198_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38042.1"
                     /translation="IRLENERIDRVRQDLARQQEQLKTVQERMAREEAERREKEKNAQ
                     YAPKKDVKEVVLPPTSYANG"
     gene            17366..>18538
                     /locus_tag="PK198_04585"
     CDS             17366..>18538
                     /locus_tag="PK198_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRF38043.1"
                     /translation="MVDQELTFYNSYFDKIEGISLLNGFLYDGAANSYGGRISLKRAN
                     STVEFEVLIPGDYPFREIAFKCTDLNGYPHHNFDGTVCLNTPFVNHIYTRLGLDIEKL
                     KGWVQKYVENEETDEHYEYMPGDCSGKIDFLFQEGEFDPNRFSQKVFGDMSFSILNAH
                     FKEQKIERTSIICQNIGNKEITWASRYKKYEKYKGIWAYIDKEPVLARKLRIENWQDL
                     IPLLPHDFLSFFETFRKKTGNYKLGPKTFQNKIFIALGYSIPSTSGGKEITWDLILLP
                     ADTFPKKDCTNALKLRRLDQKINWERTSNCDFQRFFGRGALSDKMKSSKVLILGVGAI
                     GSSIAETLVRGGLKLLSINDNDTVESGNICRSVFSFTDIGCGKAAELKGRLEQISPF"
     assembly_gap    18539..18573
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18574..19250
                     /locus_tag="PK198_04590"
     CDS             <18574..19250
                     /locus_tag="PK198_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38044.1"
                     /translation="CSNKSSTYEQLKQRLNEFDIIFDCSANNGIIEMLSEMNLNNQIF
                     YISITNKAKEMLCISSADCRSMVERRNQMMYALDAIQEAAFREGTGCWHPTFEASFFD
                     INQLLHFTIRKINTFFVKNSPPKSFYSYFRNDTIASSEDIRFVQKEKGLFITIESVCL
                     DKIFNIIRHHWPKEFGGIFIGNYINDFKEVVISDIITPRNYQNSYAGFKPDHIDLNKQ
                     LKDNGS"
BASE COUNT         4675 a         4394 c         4553 g         4893 t
ORIGIN      
        1 tatttctcac aagcccacgc cgcaaagcgg cgattcttca cgaaccattg tatcaacatc
       61 tacgtcaata tcattagtaa ggggtacatt ttggatttgc gttctaaatc taaacctctt
      121 attcggcaca tcactgtttt gaagcgctct attggtgttt gcataagccc actccagagc
      181 taagggccat agagccaact gcacctgtcc caatggtggc acgtcaaatg ttaccgcagg
      241 aaatctgttt tgtagaaacg caagtgcatt gtccgtcaca atggacaaaa catcaattac
      301 tgccgggcaa gtattaaagc cttcgtcaca aatttccgga gttgacattt gtgccgcttc
      361 tattccatcc ataccgcagt ctgcaactgc tgtttctgaa tgatctttta gcattaccaa
      421 tgcaaattct tttcccaacg gagcgattga gtaatatttc gttggcgttt gtattccacc
      481 aataaacccc tcagggcgtc ttaacaaaaa aagataacca taactctctt tcaactcgcc
      541 ataccatgtg taattatccg gggcttcctg ttcaaaatcc tttacaacaa atgtatattt
      601 tgaagtatct cccggtaacg acacagaaat acggttgttg gaaactgcat ctcgtatatc
      661 tttaatagcg accacctcac tcgctaaaga accgccatcc agcctccaac cggatagtat
      721 ctggcgttga gaaatattga ggtcaatatt ttcatttttt agatcaaata actccttagt
      781 aaaatttatg cccccctgtc gcccttgtgc cttcataagt aaaggcaata ttatcacaag
      841 gaatagcagc atggcctttg agccaatgct caccagaaat atggatggtt gtgatctcat
      901 tgtttattgg ttttgatgtg atacaatgac tggcaactca ataccctcct gcaccactgc
      961 accgcagcga tgaaatcata cagcacactt cacagcgccc taagtgtcct gtccgccttt
     1021 ctaagtgttg ttcgttgatc cgggccttat ctttacttca aatgctaaaa ctgtcggagc
     1081 cttcggattt gtgtttgttt ttcaatgaag caaatgtaca aaaaaaaacg accctccaat
     1141 ttttttaaca tttatttttg taagaataaa aaaaggagaa atagtatagt gtaaacacat
     1201 caagcagtgg ggcaaaaaac aaaattcgca aaccaccggc tattgaatat atctgcggcg
     1261 gttttgactg cttgggcagg tagaatccgc cggatagcag cacgaaatgg aagcttgcgc
     1321 cggcgggcgt agatgaggat ggaggcagac atggcgctca aatttcgcaa aaagaaagac
     1381 aaaatgtagc gcgaaccgct ccaatccaaa tcttttcgct tatttttatc tgaacaaatg
     1441 ccaaacgctg tctgtattta caaagcatcc ttatgccatg aagaagatac tcttagttgc
     1501 tgtcgctgtt ttttgcactc aattggctta ttctcaagct gatacgccga gggttaagtc
     1561 gctgccgcaa gaggccaagg gcattgtata taacaaggag ttcaccgttg atttcaaaat
     1621 gcacacctcc gggttcgggc tgggcgccaa ttggggcaag ctcaaaactt actaccgcac
     1681 gcgctttttc aatctggaac tggcagagat caaacacccc aaagagatac gccagagttt
     1741 cgactttcgc attccaagtt cgggccgcat ttcccgctcg ttcatcttcg gcaaacagaa
     1801 caatctctat gtgttgcacg gcggcatcgg tgagaagcgc tatttttccg aaaaagccat
     1861 caaccggggg ctggcagtgg gcatcagtta ttccggtggg cctacgctgg gcttgctcaa
     1921 gccgtaccat ttagatctga ttcgctttca ggagcaagac cccacaaagt tttttatctc
     1981 cgccgagtct ttttcagagg aaaatgcaga caagttttta gacctgaaca gcatctatgg
     2041 ggcatcaggc ttttcgcgcg ggttcgacca gatttccatc tctccgggcg cacacgccaa
     2101 gtttgccatc catttggatt ggggcgctta tgacgaattt gttaaatcca ttgaagccgg
     2161 cgttatggtg gacgtttttt tccaaaccat tcccatcatg gttgaatctc cattggtacc
     2221 cgacaccgga aatcaatcgg tattcttcaa cctgtttgta aatttgcagc tcggcaagcg
     2281 ccgttaatta ccattatgat agagttgaat attgtagaat ccaaagaaga aagacaacgc
     2341 aagcccgact ggctgcgcgt gaaactgccc attggccaaa actaccgcca tgtgcggcaa
     2401 ttggtagatc agtacagcct gcataccatt tgtcaaagcg gtaactgtcc caatatgggc
     2461 gagtgctggg gcgcaggcac tgctaccttt atgattttgg gcaatacctg tacgcgttcc
     2521 tgttcttttt gcgccgtaaa aaccggtcgc cccaacgaat acgacgaaga cgaaccgcgc
     2581 agagtatcgg aggccatccg gctgatgggg gtcaaacacg ccgtcatcac ttccgtcaat
     2641 cgcgatgaat tggccgaccg tggcgccgaa atctggtacc aaacggtgcg cgccatcaag
     2701 gaaagtacgc cgcaggtgac catcgaaacg ctcattcccg acgtcaagtc cacctggtcg
     2761 gcgctcgaac gcatgatcag cgcggggcag gaggtggtgt ctcacaatat ggaaaccgtg
     2821 caacggctgt atcgcaaagt gcgcccgcag gccaaatacg agcgcagtct ggagcaaata
     2881 cgccgcacca aggagtacgg caaacgcacc aaatcgggca tcatggtagg attgggtgag
     2941 acagaggagg aggtttttca aatcatggac gacctgttgg aatacggctg cgatgtgctc
     3001 accatcgggc aatacctgca acctacgaaa atgcacatcg aagtggccga gttcatccac
     3061 cccgaccaat ttgcccgcta tacggaagtc ggcctcgcca aaggctttcc ttttgtggag
     3121 agcggtccat tggtgcgttc ctcttatcac gccgaacggc acctctaaac gcggccctac
     3181 atgaatcaat ccctgcgcat tgcccggata ttcggcattc ccgtacaagt acactggtcg
     3241 tttggcctgc tccttgcctg ggtagcctat atggggtatg aacaaggctg gaccctgcgc
     3301 gagacgctgt tcatgggcgg ttttgtgctg gtgctgttca tttgcgttat tctgcacgag
     3361 ttcggtcatg cgctcaccgc gcgccggtat ggagtaggca cccgcgacat caccatttcg
     3421 cccattggcg gcattgcccg cttagaccga atgcccgaaa agccttatca ggaatttatg
     3481 gtggccattg ccgggccatt ggtcaatgtg gccatttccg gcattctttc ggtggtgtat
     3541 ctggtatggt cgggtgcagg cagtcaggca ttgcagcact ctttgtacgg catcattttg
     3601 cgcgacagca actttctgcc cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnggccgcgt attgcgggcc ttgctttcgg tgcgctgggg acgctctacg gctacgcgca
     3781 ttgccgccgg actggggcag ttgctggcag tgggcctggc gatttacggc gcctacacag
     3841 gcagttttgt cactattctc attggaggtt ttgtttttct ggccgcagcg catgagcacc
     3901 gggctgtgcg agctgaagag ttcctttccc actacaaagt atcggacgtc gttcgcacgc
     3961 agttttcccg ttttttccct ttcgatccgg tgtcattgcc tgcagaggcc atgcaacacg
     4021 gattggagca atatgtattg gttttcgacc ccgcttatca ggtggtaggc agcctgagcg
     4081 aaacggtcgt tctcatggcc gtcaaagaag aactgtacga cgcccccatc gaatcgctgc
     4141 cgctgctgcc cgcgccggaa gtgcggcccg aacaaaccct gaaagaggta ttgcatattt
     4201 ttcagacgga aaccgccccc atcctgcctg tggtggaaaa cggcgcgctc atcggtgtag
     4261 tggatcaacc gatggtggag cattttttga gaaggcagag gaaaagataa gcataatggg
     4321 gaaagccgct ctccgaaacc gtagatatga taggcagaga ttttacactn nnnnnnnnnc
     4381 catattattg atttcagccc gcacttgctg aacaaaagac tcctctactt cgagtacctc
     4441 gctgatgact ttatcgtcaa agcctttcag aatcatattg cggcaggcta agtaggattt
     4501 atggcgaata cctatttgtt cacctttttg tatgccgcgt ttttcatata gatccagcac
     4561 gctcatggcc tcggcttgaa cttcgttcga gagttgttcc aatacctttg tcatgttctc
     4621 ttcatttttg atgcggttga aaaagtaaac gaataaagct ttgaaatagt tgccatcgtc
     4681 atcaacggcg ctttccagca aaaataccgc ttgattttgc aaataagcct catcaaaact
     4741 atgcttcagt gtcaacagtg ccgagatcag cagatggttg cggaaagcgg ccagtatttg
     4801 ttcatctgga tttttttgga ggttgtgaaa agcatattcg aatttcgaca aataacacaa
     4861 gatgtcttcc ggcagatgac cgaagtgctt cacaataggc ttgtactccc aaggttcctc
     4921 tccatgataa aacacaacag gaaccaccaa acgcggcgtc tgcccgtttt gagcttcccg
     4981 ccatagggat ccgctgatgt aatacaacaa ttgaaagggc gtatttttgt caggatgact
     5041 cttatgttcc aacagcaagc acagatacac ctctgtctgg tctttcaacc gaaccgaaaa
     5101 aaccaaatcg gtataggagg cctttagtgt gtcatccacg aatgtgccgt cctcccgttg
     5161 gaggctatcc cagtcaaccc gggaacagag ttcaattggc agatatacct cgaagtattc
     5221 cctgacccgc ccaagatcgg agaacaggtg cttgaacaaa gcgtcgtgag tgcgtacgga
     5281 gtttggctga gacatgaggc aaagatgagt attccagggt ggtttggggc aaatgccttt
     5341 gaaaaacaag cacttgcccc tgtatttgtg tatattactt acccgcttcc ggtcggaggc
     5401 tgcgtaccgt gcggccggcc tgccacattt cgctttcgcg gagttcgcgg agttcttctt
     5461 ccaattttac gcggtagtcg ggttggctgt tggagtcaat agagcgctgg gcttcctcgc
     5521 cggaggccac gctgttgtac aagtcttcaa acaccggcgc aacggcatcc cggaagcggc
     5581 cgtaccagtc caaagccccg cgctgtgccg ttgtggagca gttggcgtac atccagtcca
     5641 tgccgttttc ggctactaag ggcatcaggc tctgggtaag ttcttccact gtttcgttga
     5701 aggcttcgga gggcgtatgg ccgttgcggc gcaaacactc gtactgagcg gcaaaaatac
     5761 cctgaatggc ccccatgagc gtgccgcgtt cgccggtgag gtcgctgtaa acttcccttt
     5821 tgaaggtcgt ttcaaacaaa taccccgaac cgatgccgat gcccagtgca atgacccggt
     5881 caaaagcgcg gccggtggcg tcctgtgcga tggcgtagct ggaattgaga ccgcgacctt
     5941 ccaaaaacaa gcggcgcagg ctggtgcccg aacctttggg tgccaccaaa atcacatcga
     6001 catcggcggg cggcacaatg ccggtgcgct cgctgtaagt gacgccgaaa ccgtgagaga
     6061 agtaaagcgc tttgccggcg gtcaggtgag gcttcagtac aggccaaacg gcaatctgag
     6121 cggcatctga aagcaggtat tgtatgatgg tagccttttg ggcggcttcc tcgatgtcga
     6181 agagggtttc gccgggcgtc cagccgtcgc gtacggcttt gtcccaggtg gcggagtctt
     6241 tgcgctgccc gatgatgacc ttgaagccat tgtcgcgcag gttgagcgcc tgccccgggc
     6301 cttgtacgcc atagccaatg atggcaatga cctcatcttt gagtatttcg cgcgcttttt
     6361 ccaatgggaa ttcctcccgc gttacgacgt tttcttccac gccgccaaaa ttcagctttg
     6421 ccatgatgtt gcttgtttag agaaaggtta acgatgattg atctgaatgt tcatgtcctt
     6481 gagataagta ttgaggcgct ccatagcctt cacaatggca atgcggcccg aacgaacaaa
     6541 ttcgtaaatg ccgatttttt ccaaatcctg aagcaaagct tctgtctctg aggcatgccc
     6601 ggttttttca atgaccacat attccggctc gatacataga atacgggcat tgtgcgcacg
     6661 aatgagttgc tctaccgtat cgccattgct gaatgcctct gtggggactt tgtacagcgc
     6721 aatttcctga aaaacaatgc cgtcgcgctc ataatgaaac gccttgagca cgtccacctg
     6781 cttgtccaat tgagcgacga gcttgtgtac catgtcttcg gtgacattca ccacaatggt
     6841 gaatcggtgg ataccgggaa tagaggattt ggaaacagtg aggctttcaa tattgatgtg
     6901 cctgcgtgtg aacacggcaa cgatgcgctg cagcagtccg gtttgatttt ccgaaaagac
     6961 agtgatggtg aattcctttt ccattgtgaa gcacggtttt tctgtttgtt gacaaaagca
     7021 gcatcaccgg ctttatttgg cagagcatca aaaaggggcg ctctgaaatc tgcgcctaaa
     7081 aagaaagcca ttcccgttgc caggaatggc tatattgttt tacttccgct ggagcagaag
     7141 attatttcac gtcgctgaaa aattctttga ctttgtcctt gtggacaatc acttctttgt
     7201 aagtgtattt gcccgttttg gggtctttga ccggctttac cacacgtaca tggtctttgc
     7261 cggaacctgc gttggcggaa cgttgagcta cacgcgcgtt tttagatact tttgccatgt
     7321 tgcaattttt ttaattggtt tcggaaatca ttgatacgtc gaaaacaacc ggccaatgcc
     7381 gacaatagtt cccttacttg atttcgcggt gaacggtttt cttcttaaga atcgggttgt
     7441 acttcaccaa ttccaaacgg tcgggtgtgt ttttgcgatt cttttgcgtg atatagcggg
     7501 aagtgccggc catgccggag gctttgtgct ccgtgcattc cataatgatc atgatgcggt
     7561 tccctttttg cttcttagcc atggctgccg atgattttca gatggtagat ttacttgagt
     7621 ttaacgccag tgtcaacgcc tttcttttcc aatttcttca gataagcgta cagacccaac
     7681 ttgttgatgg tgcgcacttc ggaagtacaa accttgagcg aaatccattc gtcggtttcc
     7741 ggtatataga aacgcttggt ttggatgttc ggcaaaaaac ggcggcgtgt tttgcggttc
     7801 gagtgcgata cggaattgcc cgatacaggg cgctttccag ataattgaca tactttcgac
     7861 atgacgcgtg gtatcttttc ttttccctcg gtaattaaag ttgagtgact ccggcaggac
     7921 tcgaacctgc aaccttctga tccgtagtca gatgctctat ccaattgagc cacggagcca
     7981 ttttttcaaa tgagagcgca aagatacaac catttttcaa atcggcaata gtaccgccca
     8041 aaaaaatatt cagttttttg gggcaggtct gtgattatca acaaaaaagc cgcccggaaa
     8101 gattcccgga cggctcgtat ttgccagatg tatcatagtt gagagggggc gatggtatta
     8161 tctgcccagc ggaaacatca gtccggcgtt gatactgaac ccgcgattgc tcacgcggtc
     8221 ttcaatcacc ggaatgtcat acacttgggt gaaactgtgg gaataacgcc cgtcgaagaa
     8281 gagtttcgtg ccgcccagat tgatgttcac gcctgctccg gccacaactc ccacttcgag
     8341 gcgctcataa ttaacggcgt cgaggttgat gggggtgtcc aatactttta cgtctatgag
     8401 caacagtttg gcgcgggtga gcaggcggcc gttggtnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccg ccgccacatt
     8701 aaagccgctg atggttttga aatcgggtgc taatgcgtcc aaaccttccg aagcctgcac
     8761 gttgctccag gtagcgccgg tgcggaagcc cagggaaacc tgagcagaca gggtgatgcc
     8821 gaggcaaaaa gcgatgctga aagcggccaa ttgaagcgtg gttttcatgt ctgttctgtt
     8881 ttgatgatga ataaaaaaac aacgacacaa agatgcaggc atcaagtgtt aagaggcgtt
     8941 aagcgcaggt tagcgatagt ttaatcccgg gccggcttgc ttagcatttc gttaagcccg
     9001 ccggagatgc tacctttgcg gccatatttc agaccatgca cgatctcccc aaagagcgaa
     9061 aaacattttt tttcaccgat atggaaggtt ccacacggat tgcccaacgt ttgggcgaag
     9121 gttacgccgc cgtactggag cgctatcggt ccatcattcg ttcggcggtg gggcatcatc
     9181 acgggcagga agtggacacg gccggcgacg gtttttttgc agtattcaat cacccctccg
     9241 atgcggtagc ggtggcggta gccgtacaaa gagccttctc cgtagaaaac cgaaccggca
     9301 gcgacaacta ccatgtgcgc atcggcatac ataacggaga agccgtccgc tcgcccgacg
     9361 gcggcttcat cggcatagaa gtgcaccggg cgagcagggt atgcagtgcc gctcatggag
     9421 gccaggtgct gctgtcggaa gcctcggcgc aggcagtaca acccaagctg cccaccggca
     9481 cagaactggc ccaactcggc gcctacctgc tcaaagattt cgagaaggcc gaaaacctgt
     9541 accaactcgt cattcccggc gcaccctcag agtttccgcc gccccgcacc tccatccaaa
     9601 cacataccat tgccgtgttg ccctttcgca ataccggcgc cgataaaaaa tgggacaact
     9661 tttgcgacgg catggccgag gagatcattg tggtactggg caaagtgcag ggtctgcatg
     9721 tcgtgtcgcg ctcctctgta tttgcgctca aaaaccgcga ggaaatggat ttgcaggaaa
     9781 ctgcccggca attgcacgcc accgtgttgc tcactggagc cgtccgtttc tcggggcggc
     9841 ggctgcgtgt cacggcagaa ttggtggacg tagaatcggg tctcaacctg tggtcgggcc
     9901 gctacgaccg gctgatgaaa gacgtattta atgtgcaaga cgaaatagcc gccaatattg
     9961 cttccgcgct tcgcgtcaag ctcgtttccc ggcaggtgcg cggtatcagc agcatgcaga
    10021 ctaccaatat caacgcctac gattactacc tccggggacg caggttttac gaacagttca
    10081 gccggcaaag cgtagaattt gccgtacaga tgttccgaaa ggccgtcgaa gaagacgaca
    10141 actacgcttt ggcgtattgc ggtctggcag attgttattc ctacctttat ttatatctgg
    10201 aagacagccg ggagcacctc gatgccgccc agcaatatag tatgcgggcg ctggagttgg
    10261 accccctgtt ggccaaagtg catgtttcgc ggggtattgc cctgtcgttg gacaagcact
    10321 tggtggaagc cgaagcctct tttgatcgcg ctattgatct ggatccccgt ttgtacgagg
    10381 cctggtattg gtttggccga atttgcttca ccgagggcaa gttggaaaaa gccgccgcca
    10441 tgtttgaaaa agccaaccgc ctctccccca ccgactatca ggcggctctg ctggccggtc
    10501 aggtttattc cgatctgggc ataatggaaa aatcggaggc tgcccggcgt ctgggcgtag
    10561 aaatggcaga acaagccctc gaactcaatc ccggcgacac ccgcgcactc tacctcggcg
    10621 ccaacggatg ggccgcgcta caagaatccg acaaggccat gctctggctt caacgcgccc
    10681 tcacgctgga acctgacgac gccatgttgc agtacaatgc cgcctgcgtg tatgcgctgc
    10741 tgggcaaaaa cgacgaggcc cttacttgct tagagcattc cgttgatctg gggctgagtc
    10801 agaaaggctg gtttgaaaac gacagcaacc tggcgccgct gcgacaggag gcccggttca
    10861 gggcgctgta cgagcgtttg cagtgatgcg cccaggcatc ttgaaagaaa aattgcccgt
    10921 cggaacttgc cctttcattt ttttgttgac gaaaacaagc aaacgtacta tctttgctgc
    10981 ccgattcgtg aaggtcctgt agtacaacgg atagtatgtg ggtttccgga acccaagatc
    11041 taggttcgat tcctagcagg actaccaaga gtcgcatccg gtcccttagc tcagtcggtt
    11101 acctgcctgc cggcaggcag ggggcagcag gaggcgggtc gcaggttgaa gtcctgcaaa
    11161 atttttggtc ccttagctca gtcggttaga gcagctgact cataatcagc gggtcgcagg
    11221 ttcgagccct gcagggacca cgattaattt acgtaatccc ttgcaaatga atgctttgca
    11281 agggattttt atttgatggt acaacattgg tacaacattt ttctgtcttc gactgtcccc
    11341 gcttgtcttt gtgttgtacc catccctgac atcctttgtc tcaggatagc tggtaaacag
    11401 caaaatgcga atggtagtag tactcccctt ctatctgata taccacctgc ccatcgcggg
    11461 acatcaatac ggcttccacc cagagcaggt cgcctttttt cagcttgtgt acatcgttga
    11521 tacatataac ctggtattgg cagaacaact gcaacaactc tccttgcgcg gttacataga
    11581 ggcagttttc aatagaatct tgcatggcga atagttattt gaataaagaa gaagagagac
    11641 acccagccca gggacaggcg cctcccctct tccataggat tcgatgcgct taatggatga
    11701 cagccgctcc aaccaacatg ccgttgcttt gtgcctgcct ttcggcgcga atgacctcct
    11761 gttcgttttg gccttccggc acatacaccc agcggtgatt cagcttgatc acctctccgg
    11821 tttcctgaat ggtgtagtcg aacgactcgc attccatccg agcgacatgg ccctgcagtt
    11881 tttctccgat cagagccttg caagtccgct cgtcaaaggt ggaggtgacc gaggtgcgcc
    11941 gtgcggtagc gtagtagttg ccggtggtct gactcttgac catttctatg ccgccttgca
    12001 gcagcagtgt gtagaacgtt tcgcctgcac tgttctggcg aggtgcataa ccaacaatag
    12061 taatcatggt gaaaaaatta aaaagtgaag agatgggcgg ggcatcattc cccgctccaa
    12121 ctgagggggg gtaccgaaaa ggggtacctt gagaggctgc gtacacactc aactttttaa
    12181 aatttcgcgt ttgactgtat tttcggcgtg aaaaccggct tccgtctgag catttttgtt
    12241 cgatgtgcgg cattgggcca agagtggtaa gtccttcata ctgtactgta cggctcccgg
    12301 aaccggatat tcagaaaaaa tatagacatc gctagcatgt tcataaggtc gcacccgacc
    12361 gatggcctgt gtcagctctg ctccggtgaa atacaattga atgtcccgga taaccggatc
    12421 ataggacacc gtttgatagg acagataccc gttgcattgt accttttgat aagctacggt
    12481 atgtgcattc ggcgaaatgg catgccccag cgctttggcg ataagtatat aatgtgtggc
    12541 atccatacga ggcgtgccta caattttgag gtctttgcct ttgaggctgt tgtcgccggc
    12601 agcgtttcca aaatgaagtg ccggatgtgc tcccttgacc tgagttttca gctttttaaa
    12661 agtaatggcc ggcagatcgg gcaaaaagtc attgattgac agcaacaacg cagggttctc
    12721 tttcagctta tgggcggaaa aggtgccgtc ggatatttga atgaggttcg ctttacattc
    12781 aatgtctgaa atatccacca catccacccg gtctccatag agggttctgt agaatccttc
    12841 atgggcggta gcggaaagta tgatgatgtt tttgtcccgg ggcaaactga aatctttgat
    12901 gtaatggatg atccaatcgc cctgcgggtc cagcgtatcc agcggttctg ccatcaaagc
    12961 cctgcaatta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gactccctcc
    13141 caatgctccg gccagcaatc tgaggtcttt ggtagtaatg gatgcccgtt ccagaatgtg
    13201 cgccagacaa tcttcgtcaa caatgagggt gtctttgttc ttgaattcag cgtgcaagcc
    13261 tctgctgtgc gtcgtgaaga ccatcctgga cgagcggtag caggcatcga tatccttgag
    13321 atattcggaa atattcttct tagtgatcgg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggaattgcag gcgtacaaag atggtcggcc
    13501 ttagatgcct tggaagcctg ttcaatcaac ttgtgcgtgg gcaatagata tagcgcattg
    13561 ggcaaatcaa gcccggagac aagatgggat tttcccaccc cgacgccaac tttgaaaagg
    13621 gtgattttat gatggttcct gctcaatacc tccttcactt tcgtgtgaag ttcctgacgg
    13681 gcagtagcca atggtatgag attgcggcta tcgttgatgt acattgcttt ttgggatttg
    13741 gtaattctta catggagtaa gttgcggtaa tgtagataca gcgcatgatc gggatgatct
    13801 gcgggcaggc tgtcaaagcc cggcggatag atcgggttga gggtgctgaa gcgatagcga
    13861 acgtagtcca taagacactt tttgtcctgg ttaatcaatg gatttctgtc aatacactcc
    13921 ttaaacaagc gggcgccacc ggcaatgaat gccatgttac cggccagcca aaacaattcg
    13981 ggatgtaaga ttttgcggcg ggcggcgatg aggtcagata gcagttgcgc cacctgcgca
    14041 gcttcatccc aatcgaagat ttctatgtaa tccaatgtga ttccctcttg tgaagcttcc
    14101 ttttcgacaa aatcgggggg gatttgacca cttctaatta gaatatcatt agaactaccg
    14161 gttttccccc cgtttttgtt tttttcagtt gttgggccgg tgagcgtatg tctttgaaaa
    14221 gtacggctcc acttttttgc gtccacctca ctcaaacggt aaggaagtcc ggcctcctcc
    14281 agtgcgatgg cagtgattaa cggtaaagct atttgtcctc catgccccct gccggcatct
    14341 ttccaggcag catacaggac ttcttttcct ccaaaccaca tggatacgac atctttgctg
    14401 cgcgggtcac aacccggaaa aatgacgctg aggttatgag cgcattcttt tgccactttt
    14461 ttgtcgaata tcggctgatc caatagccag actgcccgga agcgcctttc tccatggggg
    14521 tcatcgctga aactggaata gatcaacagg ggaggcaatc catgttcacg ggcggtttcc
    14581 aatacctgtt ccggtgtcca tcctttgtca aaatccaaag ccaacagctc tgtggattca
    14641 aaatttgact ctttgcgcgt gccggagagt acgctgcaac accaggtacg actgtgcggc
    14701 gctgtgagca tccggaccag ttccggcagg gttagttcgc aagcgttgct tttgtgtatc
    14761 agtccatttt cccgaaccat gtcctgtggt ttgtttttcc ggtcttccgg actgagggta
    14821 agttttacca agcggtgagc ctgcggctca gaatttgcgc aatctgcggg cgcttgttgg
    14881 gattttatat tatcatatgg catcttacag catataattt aggtttagaa gtgcttctca
    14941 aaagcacgta tttatggtat gaaaagaaga aaaaacgagg ctgccgtacg cagccttcgt
    15001 tcgtttaaga cttcctgggt tccagcgttc cggttattgc ggcgattaac cgatggatca
    15061 tttgcaacaa caggaagagc gtcgctttgt catcggcgcc gctgctctca ggagctgcat
    15121 catccggcac ataactcagt ccctcgcagt tcaagcgata atcaatatgg ccgaaatcag
    15181 gatcagcgca agttctcacc cgctgcaaat agccggcttt ttccaatcgt tgcagatagc
    15241 gattgaggta cgtgctcttg atgcctgcct ggcctttgag ggtgcgcacg gtgatgtgtt
    15301 ctttcaggcc acgcatccgt tggatggcca acagcatagc taatgtattg ccacgctcct
    15361 cctgaagcag gcgctccagc gtttcgtctt tgatacagat cattaaattt caggtttagc
    15421 ttacaactta ccttgggtaa gtgatgaata acagtatgat aagggttcct ttatcaatgt
    15481 ggtgtgcaat ttttcaaaat ttgaaatgac ttcccgcaca actcttgtat tataaaaaag
    15541 cattttgaaa ccaccaaata aaatggcaat tatttttacc tttttatgaa aaaaaatgca
    15601 cgtaatcccc tggattgcag cacccaagag ccttccccct tctcgaccct atcaccgtgt
    15661 ttccagacaa agaattcagc tgctaaaaga agtttagatc agggacggcg gctctttttc
    15721 ggctacaaaa aggacatttg aggtcaaaga cgaccaaact caccggaagg cagattcgcg
    15781 aaattggctg cttgatagcg cccgcaaccc cccaacttta cccgctggtc aaacgcggac
    15841 attccagctg tttcagtgac aatcgctgct ttgaagggcc attttgaaga caaatgaacc
    15901 gcatttttct ctgttccaac aaggcttttg tatgccccaa accgtccggt gaaaaccatc
    15961 caaaaacggc ttgtcgtcgt ttgtctccag atgacctcgc cgaagccatt tcagccctcg
    16021 aacagacttt ttgcgacatt gcaggacaaa ggatgacttg tctccatttg tccttctcgt
    16081 gaggggagat ggcttttgga gggccaatcg ggcgtgtgga tgtgtggggt gggatggcgc
    16141 ttgttgtcgg tggatgtctg tgtttgtccg ggaatgtctg ggtggatgtt gggcacgtgg
    16201 ggtatgggag ggacatgcca aagcgatgct gaagacgggc gaccgcagcg gggttgatgg
    16261 gagtggcagt aagtgtggta aaatattatc gtaatatgat tgtaaaaaaa aactcctaaa
    16321 acttgatttt ttgaatcaac tgacttaatt ttgctcgctg aggcaaagcc gctttttctt
    16381 cattccagct ttgagaggct taaggcttct acattttcca aattctatat ttattcttgt
    16441 tcgctgtgtt gcacacagga ggtaatagat caatattgcc agaaagcagt cattgcatac
    16501 tgcgagacag gtcttcaata ctccttagtt gtgccattgt tacacaacca gtattatttc
    16561 aaaataaaat cgtttcccat gttaggactt ttcaaaagaa ccaactctat tcaatcggaa
    16621 attctttctg atcttcagag tgcgaaaaaa tcaatcaaag ttgccgtttc ttggctgaca
    16681 gacacttcgt taatacaaga acttataaaa aaaaggcgac aggggcttga cgtaaagatt
    16741 cttgtttctg caaatgagtt gaatgttatt cgttttnnnn nnnnnnnnnn nnnnnacagc
    16801 atttaggagg attcgtacaa aaatggggaa atgaggagac ttccgaagga ggctttatgc
    16861 actttaaatt ttatatcatt gatgatagtt ttgctaaatc tggaagttat aactggtcaa
    16921 taaatgccac ttccaatgct gaagcattag ataaggttga tgtttttaag aagattaaag
    16981 aatttcaagc ctatttttca gaaagtgttg acttctttgc tgccatttca aatccggaaa
    17041 ggaaaagaga tgagctggag ctcatcagga aggaaaaaca tcaggatgtn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnagat tcgtcttgaa aacgaaagaa tcgacagagt ccggcaagac
    17221 ctggcaaggc agcaagaaca attgaaaact gtacaagaaa gaatggcacg ggaggaagca
    17281 gagagaagag agaaagaaaa gaatgcgcaa tatgccccta aaaaagatgt aaaagaagtc
    17341 gttttaccgc caacctccta cgcaaatggt tgatcaagag cttacttttt acaactccta
    17401 ttttgacaaa atagaaggca ttagtctttt gaatggcttc ctttatgacg gagcagccaa
    17461 ttcatatggt ggcaggatat ccctaaagag ggcaaattct actgttgaat tcgaagtttt
    17521 aattcctggc gattacccat ttcgggaaat agccttcaaa tgtaccgatt tgaatggcta
    17581 tccgcatcat aactttgatg gaacggtctg tttaaacact ccgtttgtca atcatattta
    17641 cacgcggctt ggattagata ttgagaagct gaaaggatgg gttcaaaagt atgtcgagaa
    17701 tgaggaaaca gatgagcact atgaatacat gcccggcgat tgttcgggta agattgactt
    17761 tctatttcaa gaaggagaat tcgacccgaa tagattttct cagaaggtct ttggagatat
    17821 gtccttttct attttaaatg ctcattttaa agaacaaaaa atagaaagaa caagtataat
    17881 ttgccaaaat ataggaaata aggaaataac ttgggcatcc aggtacaaga agtatgaaaa
    17941 atataaaggt atttgggcct atattgataa agagcctgta ctggcaagga aactgagaat
    18001 cgaaaactgg caagacctta ttccattgct gccccatgat ttcctttcat tctttgagac
    18061 ctttcgaaag aagacaggga actacaaact tggccccaaa actttccaaa ataaaatatt
    18121 catcgcttta gggtactcta ttccatcaac aagtggaggc aaagaaataa cttgggattt
    18181 gatattgtta ccagcggata cttttcctaa aaaggactgt acgaatgcgt taaaactcag
    18241 aagacttgat caaaaaataa attgggaacg aacaagtaat tgtgattttc aaagattttt
    18301 tggacggggt gcgctgtctg acaaaatgaa gtcttcgaaa gttttgatac ttggggttgg
    18361 cgccataggg agttccattg ccgaaacgct tgttaggggt gggcttaaat tgctgtcaat
    18421 taatgataac gataccgttg aaagtggaaa catttgcaga agtgttttct catttaccga
    18481 tattggctgt ggtaaggctg ctgaattaaa aggcaggttg gaacaaattt caccctttnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatgttc taataaatct tctacatacg
    18601 aacagctaaa acaaagatta aatgaattcg atattatttt tgattgctct gctaataacg
    18661 ggataattga aatgttgtct gaaatgaatc tgaacaatca aatattctat atttccatta
    18721 caaataaggc aaaagagatg ctttgcattt caagtgctga ttgcaggagc atggtcgaaa
    18781 gacggaatca aatgatgtat gcacttgacg caattcagga agctgctttt cgagaaggta
    18841 caggttgttg gcatcccacc tttgaggcct cattttttga catcaatcaa ctattgcatt
    18901 ttacaattag gaaaatcaat actttttttg taaaaaactc ccccccgaaa tcattttatt
    18961 catactttag aaatgataca attgcttctt ctgaagatat aaggttcgtt caaaaagaaa
    19021 aaggactttt tataactatt gaaagtgttt gtctcgataa aattttcaac attattcgac
    19081 atcactggcc caaagaattt ggcgggattt tcatcggcaa ctatatcaat gactttaagg
    19141 aagtagttat ttctgatatt attactcccc gaaattacca gaacagttat gccggattca
    19201 aacccgatca tattgattta aataagcaat tgaaagacaa tggttcgtga tatactcacg
    19261 ccgcccaggc ggcgtaataa ttagaaaata a
//
LOCUS       DAORLF010000031        19259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1523_length_19259_cov_1.975578, whole genome shotgun sequence.
ACCESSION   DAORLF010000031 DAORLF010000000
VERSION     DAORLF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19259
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_19259_cov_1.975578"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..501
                     /locus_tag="PK198_04595"
     CDS             196..501
                     /locus_tag="PK198_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38045.1"
                     /translation="MRTNRFPLRLMSFAAVAVLGFVLVSCAGVEPVQECLKGQQYGFL
                     HGLLHGFITPVSFVVGLFNDSVAIYAVNNSGGWYDFGFLLGSGGWGFLAGNKSGKKS"
     gene            complement(703..1629)
                     /locus_tag="PK198_04600"
     CDS             complement(703..1629)
                     /locus_tag="PK198_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRF38046.1"
                     /translation="MSNQTTDTILMVRPARFGYNEETAANNAFQTNDRSRSQSEIEQA
                     AQAEFDAFVERLRACGVRVIVAQDSPEPAKPDAVFPNNWVSFHHDGHIITYPMFSPLR
                     RLERREEVIAQVLESGFRADRRTHLETSEADNRFLEGTGSMILDHDHRLVYACLSQRT
                     DAALLDDYARLIGYQAVAFHSTDAQGQDVYHTNVMMALGETFAVICLDSVKDAAERAM
                     LEARLTGTGKTIIDISLDQMNRFAGNMLQVKSTDGQAFLVMSEQAFKSLTPAQVLAIE
                     AHTSILHAPLYTIEQYGGGSARCMLAEVFLPK"
     gene            complement(1672..>1865)
                     /locus_tag="PK198_04605"
     CDS             complement(1672..>1865)
                     /locus_tag="PK198_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38047.1"
                     /translation="KEMKRLQADNAKFHKEIEDAKARIKKAEENIAQNEKDQRQAEDK
                     IANQKKNVEAVRRRLEQQR"
     assembly_gap    1866..1902
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1903..2356)
                     /locus_tag="PK198_04610"
     CDS             complement(<1903..2356)
                     /locus_tag="PK198_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38048.1"
                     /translation="MKKQLSVLVFLLIGLAGTLSAQVKEMEKNMVLGLQNALVLDIPQ
                     ADDKLVARVWNNYVKDYYKGKNKQVKGGGEIISSELSVPSLNPGGTVDLYALPEKAGN
                     DVRFALWIKMPQGFLSSHAFPDQYRDAEALLKRFGVEVNKEKIRMELAD"
     gene            complement(2463..3548)
                     /locus_tag="PK198_04615"
     CDS             complement(2463..3548)
                     /locus_tag="PK198_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF38049.1"
                     /translation="MRAVHLIIGIGLLILTACTEPDTGHPAQTPFSPQPYEHQDPPGF
                     VPMLLPADNPMTVEGVELGRRLFFDPVLSADNSISCASCHLPALAFTDGKAQSNGVLG
                     RRGMRSSPSLVNVGYYYKGLFWDGRVMTLEEQSLHPVADSLEMAASWPEIEQRLRSHS
                     EYPALFRRAFGIPKAGEINRYWVGKALAQYQRTLVSADSKFDKKTRGETTFTEMEQRG
                     WTIFFDASPSVPNSECGHCHIDPLFTTLEFHNNGLDAPRDPFIFPDPGRGGVTGNRFD
                     NGTFRVPTLRNIALTAPYMHDGRFATLEEVIDHYASGGHYADNLNPNIMELNLDARDK
                     AALIAFLHTLTDDTFLAKHAGDGEGVR"
     gene            complement(3588..4043)
                     /locus_tag="PK198_04620"
     CDS             complement(3588..4043)
                     /locus_tag="PK198_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF38050.1"
                     /translation="MKSIQLAFCLFLLMAVLPAQAQTSNSTSDIEQITATLMDYIEGT
                     ANGEPDRLRRAFHPDFKLYTITESDSLRIRSGEKYISDIKPGEKANRIGRIISIDYEK
                     DAAMAKAEIVIPGWRIFTDYFLLLRYEGSWKIVQKSYTWRAYPKAEEKK"
     gene            complement(4177..5331)
                     /locus_tag="PK198_04625"
     CDS             complement(4177..5331)
                     /locus_tag="PK198_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF38051.1"
                     /translation="MFNLANFRQYKSVRATGFSFAGSGLVFGTWAALIPWLKYQYGLD
                     EAQLGLLLLCPPLGIVLANPVSISLLARYGAVRTTLGGLLAGASMLLISIVSPTLWLT
                     VPFLFLLGGCMAILNVAMNTCAAMLEQEKGLRILSTCHGLWSSGAMAGSALAGTALGL
                     GITAPVYVAGLLGFVILLVFQLKRPMSEVPEKAASHSDTDSPKGFTWPSSALWLLIIL
                     SLCTNLTEGTISDWGAVFMREETGVGEAVASWGFAVYAFFMAGGRFLGDEIIARFGGK
                     TVLRAGGIIAATGLMPAVFFQHPALVLVGFALVGAGVSLGAPILYAAASRVPGMAKGA
                     GLATMNTFAMAGFLGGPALIGFIAKAFSLPFAFGVVAVLTGIWIWKAGKV"
     gene            complement(5340..6311)
                     /locus_tag="PK198_04630"
     CDS             complement(5340..6311)
                     /locus_tag="PK198_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38052.1"
                     /translation="MTHKAILSGRLEFGNARSCEKAMTLFQSRYEVMYRSDVFLKVDD
                     VFHVETSTLDLYRFTAQVAEKTWNNTMSLLEEVSQFAMTGKIDAWLTDNGEVLRAKFI
                     EPVTEKAAVQAFLKGRELMKQQGHEQEAIGSLNDAITKYERHAAAYERRGYVNYRLRN
                     YPDAVYDYSKSIAFNPACAEAYLGRAVVYIAQDNPAAALPDLEKALNFSVPLQPIFWI
                     ARRLKGDCHLDLEQYDAAVTEYQFFLKRKFSAGDVNFNRRRRVSYYLGKALLALRKNK
                     EAVLALETALKTDTLDSAAAEADIQNWLQEARQRSTPAAGSRASVAV"
     gene            complement(6746..7762)
                     /locus_tag="PK198_04635"
     CDS             complement(6746..7762)
                     /locus_tag="PK198_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRF38053.1"
                     /translation="MHTSDSLNFLSKLTLRRCANALAVLGSYYAAKILRRPIQWGLPF
                     SISVEPTTACNLRCPECPSGLRAFTRPTGNLRADFFRNVMDELASDLLWLIFYFQGEP
                     YINPAFLEMVAYAKSKKIYTITSTNGHFLDDDNARRTVESGLDRLIISLDGTTQEVYE
                     QYRKEGRLDKVLQGAANVVKWKKALRSSTPHIVFQFLVVRPNEHQIPEAYRLAREIGV
                     DEVKLKTAQIYDYENGHPLIPTQEKYARYRHNEHGRYEVKNRLVNHCWKLWHSCVVTW
                     DGVVVPCCFDKDASHRMGSLRQQPFRSIWHSPAYRQFRTAILKGRDQIDICTNCSEGC
                     KVWV"
     gene            complement(7774..8640)
                     /locus_tag="PK198_04640"
     CDS             complement(7774..8640)
                     /locus_tag="PK198_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF38054.1"
                     /translation="MPNFSKAIAGIFRLSKRISFSPQGFAELHRLQNFPSVHLQRQVV
                     IDVFVPPSYHHQPERAFPVLLLNDGQDMEAIQMGDILERLYQQRRIPEILVVAVHTGI
                     QRLDEYGISQRPDYKNRGAKAAAYAQFITKELLPALQLRYRCSELHEHWAVAGFSLGG
                     LSAFDIGWNHPHIFSKIGVFSGSFWWRSQVFQEHNPDGHRILHELLRESKKRERLRFW
                     LQTGTEDEASDRNHNGIIDSIDDTLDVIRELKALGYNEQRDIRYVELIGGRHDLPTWA
                     KIMPDFLQWAFG"
     gene            8791..12018
                     /locus_tag="PK198_04645"
     CDS             8791..12018
                     /locus_tag="PK198_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF38055.1"
                     /translation="MKAFFTSLLLLTLTAVWGQRNCGTMDVLTEQLQQNPALQQQMQS
                     IEQHNDHYHNHLHEQVGFRAVVTIPVVFHIIHNGDAVGSNENISDALILAQLQQLNDD
                     FRKLNTDAGLVPALFQGLHADTEIQFCLAQRKPDGTATNGINRVQYSQATWTTSQVNS
                     TVKPATIWNRDQYLNVWSVAFGGSSSGILGYAQFPGGAASTDGVVVAYYSVGSLAMTN
                     PGSSTFGKGRTLTHEVGHWLNLRHIWGDATCGNDLVADTPVHNASNSGCPTYPHLSTC
                     TGTPVEMTMNYMDYTNDACMYMFTAGQKTRMQAVLATGGARFSLNSSLGCVPPGETNC
                     TAPTLAQLSASNITTSSARINCSVTGVNAYDWRYRVAGTSTWTDLASGTPNFADLSGL
                     AAGTTYEFQAQVQCGSVWSAWSVTQTFTTLSAACAAPTLAQLSVSNITTTSVQLQCSV
                     SGVNAYDWRYRQQGAATWIDLPSTTTGVTTVTGLSASTNYEFQAQVQCGSTWSAWSVS
                     RSFSTTDGSCTTPLASQISITEITGSSARFNVSVTGANSYLWRYRQAGASTWITLPAT
                     TVNYTYVFGLSSRTTYEVQVALVCNSITTAWSGTVNFSTSAGGDPVCNAPDVSQLSAS
                     NVTTSSAVLNCTAPGATSFTWRYRVAGANTWNNLGTTTQGTFALSGLSPNTTYEFQVM
                     LECSNGVASSWSASQSFTTPGAATCNAPTAAQISATNISTTSAQLNCSIAGQSSYAWR
                     YRLAGAATWTTLPSTSVNSTVVNGLSASSNYEFQVSIQCTNGVWSDWSGTQTFSTAAA
                     ACNAPSVAQVSVSNITDASARLNCSVTGVTAYLWRYRQAGASAWIDLGSTSVNFYDLG
                     NLSSSTTYEYAVAVQCGAAISAWSVTQNFTTTVILNCTPPTARMLFVTGIAPTGATLN
                     CSMSATNYLWRYRPLGTSVWITAGPTTVGSLTVSGLMPQTTYEFQVAVLCGTTQSDWS
                     DSKTFTTSGGTRIEPNHLLRMELWPVPAHEQLFIRYTTPEDATVVITISDMAGRVVLT
                     ENKGRQTAGEHLHEMRLEQLPAGTYSIQIQTGKNICREKFIRFAE"
     gene            12062..13045
                     /locus_tag="PK198_04650"
     CDS             12062..13045
                     /locus_tag="PK198_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38056.1"
                     /translation="MKKIGILYGWERSFPAAFVERVNSKKVEGIIAEPVQISQVRQAE
                     STGYSVIVDRISQDVPFYRAFLKNEASAGAAVINNPFWWSADDKFFNNAVAVKVGVPV
                     PKTVILPSRDHPTDTKAESFSNLSFPLDWEGMFQYIGWPAFMKPFAGGGWKHVYKLDS
                     ANDFFAKFGETGQLVMLLQEAIEFDAYFRVYCIGAKHVRIMHYEPRNPHHLRYSANHK
                     VSDELLKTIEDYTIRLNVALGYDFNTVEFAIRDGIPYAIDFGNPAPDADRHSVGDDNF
                     EWVLEHSAQYAIERALAHQEGGDNLTWGTFVKNSAAANAAPAPKKAAAKKK"
     gene            13151..13978
                     /locus_tag="PK198_04655"
     CDS             13151..13978
                     /locus_tag="PK198_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HRF38057.1"
                     /translation="MEPVRLAVLDLYDNTPNLGMGRIKELVEMFGNAYEYDVFDVRGK
                     AEVPDMSYDVYISSGGPGSPHDGDGVWDEKFFHWMDEVWDWNASGEMPKKYVFFICHS
                     YQMACKHFGIGTVSRRRTPSFGIFPTHKTEAGKMEALFDGLPNPFYIADFRDWQVVEP
                     DMRRIDELGAEILALEKIRPNVPLERAIMAVRFSPEIFGTQFHPEADAKGMLHHFSDP
                     ERRIKIIADHSEEKYLDMIEHLNDPDKISLTQRIVLPLFLSRSLRAARAEKMMPELV"
     gene            14002..14163
                     /locus_tag="PK198_04660"
     CDS             14002..14163
                     /locus_tag="PK198_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38058.1"
                     /translation="MIPELRNHYNAAFTQARYEAFIQEMVQRYEHRPSFNVAETPVFI
                     PTDLRDRLV"
     gene            14176..>14428
                     /locus_tag="PK198_04665"
     CDS             14176..>14428
                     /locus_tag="PK198_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38059.1"
                     /translation="MCSLFLRPDFKALSEGALQPGQVVPNESEHSEFFVIDFGICQDE
                     NGHLIPQLVEAQGFPSLFSYQHVLAELYKKHFDAIPAHYT"
     assembly_gap    14429..14495
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14496..>14961
                     /locus_tag="PK198_04670"
     CDS             <14496..>14961
                     /locus_tag="PK198_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38060.1"
                     /translation="VADSKPENVVLLEIEPEKQTTAIDFYATREMLGIPFVCLTKLKK
                     EGRTLYYTNENGRKIEVERIYNRIIFDDLMARPELQTEFHLTDDVDVQWAGHPNWFFR
                     ISKHTLPLFDSPYVPKSYYLDKLSAIPDDLENYVLKPLFSFAGMGVIVNPTRE"
     assembly_gap    14962..15112
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15380..15499
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15500..15934
                     /locus_tag="PK198_04675"
     CDS             <15500..15934
                     /locus_tag="PK198_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38061.1"
                     /translation="QEEQAKAERLEVLETKMDALAELLVAEPADTIEAYDQAKAALLH
                     HIYDVEAALKNAYAEAGASLRQRLDGFRTALDGYRIPLALSSFDNQQDALSKKEELNA
                     VLAELLGHFHAEEKNEDKLEHFMSEMETAFEHVKKAVKELIK"
     gene            15937..>16186
                     /locus_tag="PK198_04680"
     CDS             15937..>16186
                     /locus_tag="PK198_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38062.1"
                     /translation="MTNNAKHKNSPSLLARYEKVISVLLKYGFEDVAAHPPFNRFLPK
                     LNKIIPLRDGKPVYEYTRYERIRMVCEELGTTFIKFAQI"
     assembly_gap    16187..16196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16197..>17040
                     /locus_tag="PK198_04685"
     CDS             <16197..>17040
                     /locus_tag="PK198_04685"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRF38063.1"
                     /translation="NRPDVLPEALLSELEHFQDRVPFVPSQDIVSVIQEDLGKLPEEL
                     FEHFDNQPLASASIAQVHRARLIGGKEVVLKIMRPGIDVTIEEDIHILKQLAGLMEAY
                     FPQYQAFQPMELVKMFERSIRKELKFSIEANNLRRFAQQFRGHEGIYVPDLYPEFCSD
                     RILCMEFIDGVKITDPEAISRCGFTGPELALAGINLYFEQVFTHGFFHADPHPGNIFV
                     LPERQICFIDYGMMGSIVDSDRDMLADLLLSVSNRDVDGLKAALLRFTPDGTVENEKE
                     LEYDI"
     assembly_gap    17041..17099
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17100..17633
                     /locus_tag="PK198_04690"
     CDS             <17100..17633
                     /locus_tag="PK198_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38064.1"
                     /translation="VIEGLNAMFFAYKIKIPGNLLLLLKALVIIEGVGLSLDPHYNII
                     ANIDPFVRKLLSHKYSPDKLTRKAISTLAGMTQMAANLPEDIENIIRKIREGKLCIEM
                     EHKGLDDFYRKMDEVSNRMAISILLAALILGSSLLVLAEVPPFVGNIPALGFVGFVIS
                     GLLALRLVVSVWKHGKF"
     gene            complement(17664..>18558)
                     /locus_tag="PK198_04695"
     CDS             complement(17664..>18558)
                     /locus_tag="PK198_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38065.1"
                     /translation="YATVLLLFAIAVAFSGCSGQEDICHRYLDEKHSRINAEHEQLYK
                     EGNVSEAVSFVKEEIKRDSNNYVAVSYLAAYQYFLCQQAGCSDEELKDVYELINQALA
                     LCNDYRLGYFNIIQVLSDMSATKYKNDPKLLEYLEMYNSRYPKNSKVMSAGGAALYRL
                     GRIEEAHQHVNEAISLDLNEAVAYIFKAKCYMEQNELENAMKYINLGLSIDSISLGFH
                     DRGHLHYAAGDIDAAVRDFQTAIAIDSDRYESYVGLGQIELERNNDTAACRYFEMALK
                     INPDDKRTHLMMRIYCRKHSS"
     assembly_gap    18559..18621
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18976..19012
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19013..19223
                     /locus_tag="PK198_04700"
     CDS             <19013..19223
                     /locus_tag="PK198_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38066.1"
                     /translation="KNVETEASKAKQSQADKDILFGSLWLAGGTIVTVVSMSSASGGG
                     RYIITWGAILFGAIQLIKGLVNKFS"
BASE COUNT         4426 a         5062 c         4891 g         4336 t
ORIGIN      
        1 tctaatcctt tgacgaaaat gatcacttta tttttttatc gccccttgac aatgaggtgt
       61 ttgttttcga ccaaagttat tgcatcgtct ggcagttgca tctatacatc tcccagtccg
      121 ctcacttggt cgagcacatt tgaaagggat gttgtcagga ggccaacttt gccccacatc
      181 aaaataaacc aaaccatgag aaccaaccgc ttccctcttc gcctgatgag ttttgcagcc
      241 gtcgccgtgc tcggttttgt gctggtatcc tgcgccggcg tggagcctgt tcaggaatgt
      301 ctgaaaggcc aacaatatgg atttttgcat ggcctcttgc acggattcat tacgcccgtc
      361 agcttcgttg tgggcctttt caacgactcg gtagccatct acgccgtcaa caacagcggc
      421 ggctggtacg atttcgggtt cctgctcggt tccggcggct ggggtttctt agccggcaat
      481 aagtcgggca aaaaatcctg ataaccaaat atctggtcta aggcgcgatg aaaaaaacct
      541 tcattttcgc cgcgctactt tgtgtctgtg aatttagtcg tttgtttgga ctttagagtt
      601 cgagagcaag gcttgcgaag gccccaaaat cagagtttac tggtcgtaaa tgaggatttt
      661 ggggccgagc gtaacgcagc tatcggacta taaagacaaa cactatttgg gtaaaaacac
      721 ctcggccaac atacaacgcg cactgccgcc gccatactgc tcaatggtgt ataaaggagc
      781 gtgcaagata cttgtatgcg cctcaatggc aagcacttgt gccggtgtca ggcttttgaa
      841 agcctgctcc gacatgacca aaaacgcctg gccgtcggtg cttttcacct gaagcatgtt
      901 gcctgcaaag cggttcatct gatccaaact gatgtcaatg atcgttttgc cggtaccggt
      961 cagccgggct tccagcatgg ccctttctgc ggcatccttc accgagtcta aacaaatcac
     1021 ggcaaaggtt tcccccaacg ccatcatcac attggtatga tacacatcct gcccttgcgc
     1081 atcggtggag tggaaagcaa cagcttggta gccgatgagc cgggcatagt cgtcgagcaa
     1141 tgccgcgtcg gtgcgttggc tcaggcaggc atacaccaga cgatggtcgt ggtctaaaat
     1201 catgctgccg gtgccctcca aaaagcggtt gtcggcttcc gatgtttcca aatgcgtgcg
     1261 tcggtcggcc ctgaacccac tctctaatac ttgagcgatc acctcctccc ggcgttcaag
     1321 gcggcgcagc ggtgaaaaca tggggtaagt aatgatgtgg ccgtcgtgat gaaaactcac
     1381 ccaattgttg ggaaacacgg catccggctt ggccggttcg gggctgtctt gcgccacgat
     1441 gacgcgcacg ccacaagcgc gcagccgctc aacaaaggca tcgaattcgg cctgtgcggc
     1501 ttgttcgatc tcgctttgtg agcggctgcg atcattggtc tgaaaagcgt tgttggcggc
     1561 ggtctcttcg ttgtacccga aacgggccgg gcgcaccatc agtatggtat cggttgtttg
     1621 gttggacatg gcagcgcaat tttgtgcccc gcaaagatgc gcaaaaaacg cttagcgttg
     1681 ctgctctaag cgcctgcgca cggcctccac gttctttttc tggttggcta ttttgtcctc
     1741 cgcctgccgc tggtcttttt cattctgcgc gatgttttcc tctgcctttt tgatgcgggc
     1801 cttggcgtcc tctatttcct tgtggaactt cgcgttatcg gcttgcaggc gcttcatttc
     1861 cttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatcggcc aattccatgc
     1921 ggattttctc tttgttcact tccacgccga aacgtttcag cagagcctcg gcgtcgcggt
     1981 attgatcggg aaaagcatgg ctactgagga aaccctgcgg cattttgatc cagagtgcaa
     2041 agcgcacatc gttgcctgct ttttcgggca aggcgtacaa atcaacggtt ccgccgggat
     2101 tgagcgaggg caccgacagt tcggaagaaa tgatctcgcc gccgcctttc acctgcttgt
     2161 ttttgccttt gtaatagtct tttacgtagt tgttccacac tcgggccacc aatttgtcgt
     2221 cggcctgcgg aatgtcgagt accaaggcgt tctgaagccc cagcaccatg tttttctcca
     2281 tttctttcac ttgggcagag agcgttccgg ccaggccgat gagcagaaaa acgaggacag
     2341 atagttgctt tttcatgtta gtttcatttt tttgttgcgg tattgagacg gtgattcatg
     2401 gatgaaggta acagtttttt tgcaaactca ttgtttcttt ccaatccctg aagactatat
     2461 tctcacctga cgccttcccc atctccggca tgctttgcca aaaacgtatc atccgtcagc
     2521 gtgtgcaaaa aagcgatgag cgcggccttg tcgcgggcgt cgaggttcaa ttccatgatg
     2581 ttgggattca gattgtcggc gtagtgaccg ccggaagcat aatggtctat cacctcctcc
     2641 aaggtggcaa agcggccgtc gtgcatgtag ggcgccgtca gggcgatgtt gcgcagggtg
     2701 ggcacgcgga aagtcccgtt gtcgaagcgg ttgccggtga cgccgccgcg gcccggatcg
     2761 gggaagatga acggatcgcg aggcgcatcc aagccgttgt tgtgaaattc cagcgtagtg
     2821 aacagcggat caatgtggca atgcccgcac tcggaattgg gtacgctggg cgaagcatcg
     2881 aaaaagatgg tccagccgcg ttgttccatc tcggtgaagg tcgtttcgcc gcgcgttttt
     2941 ttatcaaatt ttgaatcggc gctcacgagc gtgcgctggt attgggccaa tgctttgccg
     3001 acccaatagc gattgatctc ccccgccttt gggatgccga acgcccgccg aaacagtgcc
     3061 ggatactccg aatgactgcg cagccgctgt tcgatttccg gccaggaagc cgccatttcc
     3121 agcgaatcgg ctaccgggtg caacgattgt tcttccaagg tcatcacccg gccgtcccaa
     3181 aacaagccct tgtaataata gcccacattg accaagctgg gtgaactgcg cataccccgt
     3241 cgccccaaca cgccgttgct ctgcgccttg ccgtcggtga aagccaatgc cggcaggtgg
     3301 caggaagcgc aggaaatgct gttgtcggca gagaggacgg gatcgaaaaa cagacggcgg
     3361 cccaactcca cgccctctac cgtcatcggg ttgtcggcgg gtaggagcat gggtacaaag
     3421 cccggcgggt cctgatgttc gtaaggctgt ggcgagaagg gcgtttgtgc gggatggccg
     3481 gtgtcaggtt ccgtgcaggc tgtcaagatc aaaagcccga ttccgatgat aagatggacg
     3541 gcgcgcatga tgtgaaaaca cgcgggcgag acgaagggtt gtgtacgcta ctttttttcc
     3601 tccgcttttg gatatgccct ccaggtgtag cttttctgta caattttcca ggagccttcg
     3661 tacctcagca gcaggaaata atctgtaaaa atcctccagc ccggaatgac gatttccgct
     3721 tttgccatag ctgcatcttt ctcataatca atggaaatga tccgcccaat cctgttggcc
     3781 ttttcgccgg gcttgatgtc ggagatgtac ttttcgcccg aacgaattct caggctgtcg
     3841 gattctgtaa tcgtatataa tttgaagtca gggtggaaag ctcgccgcag ccggtcgggt
     3901 tcgccgttgg cagtaccttc tatgtaatcc atcaatgtgg ctgtgatttg ttctatatcc
     3961 gaggtactgt ttgaggtttg agcctgtgcc ggcaaaacag ccatcaatag aaaaaggcaa
     4021 aaagcaagtt gaattgattt catggcaaag aggtattggg tggtaaaaaa tgaaaagggc
     4081 ttacacgccg ggttttgaac gcgccaccgg atacaatatt tcataaaaca gtctcagccg
     4141 ttgaatcaag aacactcggg attggcaatc agcacttcac accttccccg ccttccaaat
     4201 ccaaattcct gtcaacacag ccaccacgcc gaatgcgaag ggcaaactga aggctttggc
     4261 gataaaaccg atcagcgccg gcccacccag aaatcccgcc atcgcaaagg tattcatggt
     4321 ggctaatcct gcgcctttgg ccatgccggg cacccgcgat gccgccgcat acaaaatggg
     4381 cgctcccaac gacaccccgg cgcctaccaa tgcaaaaccg acaagcacca gtgccggatg
     4441 ttgaaaaaac acggcgggca tcagtcctgt ggcggcaatg attccaccgg cgcgcaaaac
     4501 tgttttcccg ccaaaccggg caatgatttc atctcccaga aaccgaccgc cggccataaa
     4561 aaatgcatac acagcaaaac cccaactggc tacagcctcg cccacgccgg tttcttcgcg
     4621 cataaacacg gctccccaat ccgagatggt gccttcggtc aggttggtac acaaactgag
     4681 gatgatgagc agccacaagg cgctgcttgg ccaagtaaat cctttcggcg agtcggtatc
     4741 ggagtgggag gctgcttttt ccgggacttc gctcatgggg cgtttgagtt gaaaaaccag
     4801 cagaatgaca aacccaagca agccggctac ataaaccggc gcggtaatgc ccagccccaa
     4861 tgcggtgccg gcaagcgccg aaccggccat ggcgccgctg ctccacaatc cgtgacaggt
     4921 ggacaagatg cgcagccctt tttcctgttc cagcatggcg gcgcaggtgt tcatggccac
     4981 attgagaatg gccatacagc cccccagcaa aaacaaaaaa ggaactgtga gccacagcgt
     5041 gggcgaaaca atggatatta aaagcatgga agcgccggcg aggaggccgc ccagcgtggt
     5101 gcgaacggcg ccgtagcggg ccaacaggga aatggaaacg gggttggcca aaacgatgcc
     5161 caagggcggg cacagcaaca gcaggccaag ttgagcctca tccaatccgt attggtattt
     5221 gagccaggga atgagcgccg cccaggtacc gaataccagc cccgagcctg caaaactgaa
     5281 acccgtagcc cgtactgatt tgtactgcct gaaatttgcc agattaaaca taatgtgact
     5341 tacacagcca ccgatgcgcg cgatccggca gcaggggtgc tgcgctgacg agcctcctga
     5401 agccagttct gaatatccgc ttcggcggcg gcgctgtcga gggtatctgt tttgagggct
     5461 gtttcaagcg ccagtactgc ttctttgttt ttgcgcaatg ccaacagcgc tttgccaaga
     5521 taataagata cccggcggcg gcggttgaag ttgacatcgc cggcgctgaa tttgcgcttg
     5581 aggaaaaact ggtactcagt aacggcggca tcgtactgct ccaaatcgag gtggcaatca
     5641 cccttgaggc ggcgggcaat ccagaaaata ggttgcaacg gcaccgaaaa attgagcgct
     5701 ttttccaggt cgggcagcgc ggcagcagga ttgtcttgag caatatatac tacggcacgg
     5761 ccgagataag cctcggcgca ggccggattg aaggcaatac ttttggaata gtcgtacacg
     5821 gcgtcggggt agttgcgcag gcgatagttg acgtagcccc ggcgctcgta agcggcagcg
     5881 tgacgttcat acttcgtgat ggcatcgttg agggaaccga tggcctcttg ttcgtggcct
     5941 tgttgtttca tcagctcgcg gcctttgaga aatgcctgaa cagcagcctt ttcggtcacc
     6001 ggctcaatga atttggcgcg gagtacctcg ccgttgtcgg tcagccaggc atcaattttg
     6061 ccggtcatgg caaactgaga cacttcttcc agcaggctca tggtgttgtt ccatgttttt
     6121 tcggctacct gagcagtgaa gcgatacagg tcgagcgtag aggtttctac atgaaacaca
     6181 tcatccactt tgagaaaaac gtcgctgcga tacatgacct cataacgaga ttgaaagagc
     6241 gtcatggctt tttcgcaact acgcgcgttg ccgaattcta atctgcccga aaggattgcc
     6301 ttgtgtgtca ttttcctgct gttgttattg taaactcaaa atactgtctg cactgttgcg
     6361 gcgcgcaccc ggcactacgc tcatagcggc aggctgcgca atagaaacac ggtgaagttc
     6421 ccgttcgggg gtacccgtga tgtttttaag tatcagcaat aagagcgggg catcaaaaga
     6481 ggcaggagca ccggcgaatg atggagtatt ggccgccgaa tttgggtaaa cccctgaatc
     6541 tgaatcctga aaagatatga tgaatgcctg tttcgctctt taaagaccga actacaaagt
     6601 taatgcgcgg gcacacccaa atgcaagtaa attcataact tttttaaaat caattgaaaa
     6661 aaggcagggt tgaccattcg gagcttgttt tttggacggc agccagagtg cgaattaatg
     6721 aaaaaaaatt tttgtattca ttttgttata cccatacttt gcagccttca gagcagttgg
     6781 tgcaaatatc tatttggtcc cgacctttca gaatggccgt tctgaattgc cggtacgccg
     6841 ggctgtgcca aatgctgcga aagggctgtt ggcgaaggct gcccatgcga tgcgaggcgt
     6901 ctttgtcgaa acaacagggc actaccacgc cgtcccaggt gacgacgcag gaatgccaga
     6961 gtttccagca gtgattgacc aatcggtttt tgacctcgta gcggccatgt tcattgtgcc
     7021 ggtagcgggc gtatttttct tgagtgggaa tgagcggatg gccgttttcg taatcgtaaa
     7081 tctgggcggt tttgagcttc acctcgtcca cgccgatttc gcgggccaag cgatacgctt
     7141 cggggatttg atgctcgttg gggcgcacca ccagaaactg gaagacgatg tgcggcgtgg
     7201 aagaccggag cgcttttttc catttcacca cattagcggc gccttgcagc actttgtcca
     7261 agcggccctc tttgcggtat tgctcataca cttcctgggt ggtgccgtct aaggaaatga
     7321 tgagccggtc gaggcctgat tctacggtac ggcgggcgtt gtcgtcgtcc aaaaaatggc
     7381 cgttggtgga ggtgatggtg tatatttttt tgctcttggc gtaagcgacc atttccaaga
     7441 aggccgggtt gatgtacggc tctccctgaa agtaaaaaat gagccagagc aggtcggaag
     7501 cgagttcatc catgacattt cggaagaagt cggcgcggag gttgccggtg ggccgggtga
     7561 aagcgcgcag tccgctgggg cattccgggc aacggagatt gcaggccgtt gtgggttcaa
     7621 cggaaatgga gaacggcagc ccccactgta tggggcggcg caggattttg gcggcatagt
     7681 acgagccgag cacggccaaa gcgttggcac agcgccggag cgtgagtttg gagaggaagt
     7741 tgagggagtc ggatgtgtgc atgaaatctt cgattaacca aaagcccact gcaaaaaatc
     7801 cggcatgatt ttagcccagg tgggcaggtc gtgccgtccg ccgatgagtt caacgtaccg
     7861 gatgtcgcgt tgttcgttgt agcccaatgc tttgagttcg cggattacat ccagcgtgtc
     7921 gtcaatagag tcaatgatgc cgttgtggtt gcggtcggaa gcctcgtctt cggtaccggt
     7981 ttggagccaa aaacggaggc gttctctttt tttactttcg cgcaacagtt cgtgcaggat
     8041 gcggtggccg tcggggttat gttcctgaaa gacctgcgaa cgccaccaga aggagccgga
     8101 aaatacgccg attttgctga aaatatgcgg gtgattccag ccgatgtcga aagcagacag
     8161 accgccgagc gagaacccgg ctacggccca atgctcgtgc agttcgctgc accggtagcg
     8221 caattgcagg gcgggcagca gttctttggt aataaactga gcgtaagcag ccgctttagc
     8281 gccccggttt ttgtagtcgg gccgctggct gatgccgtat tcgtccaaac gttggatgcc
     8341 ggtgtgaacg gccacgacta aaatttctgg tatgcgccgt tgctgataca ggcgttccag
     8401 aatatcgccc atttggatgg cttccatgtc ctgaccgtcg ttgagcagca gtacgggaaa
     8461 tgcgcgctcg ggctgatgat ggtaggaagg cggtacaaaa acgtcaatga ccacctggcg
     8521 ttgtaaatga accgagggga agttttgcag gcgatgcagt tctgcgaatc cctgcgggct
     8581 gaaactgatg cgcttggaaa gcctgaaaat gccggcaatg gctttggaaa aatttggcat
     8641 atcactcttt ttaagcaacc tggcttgcaa aaaatgcacc tgatatctga tataggttgt
     8701 gcaaatgtta actttgcacg cccatccaat ccctgattgc agggcaaaag taaattttca
     8761 tcattatcat caaacaaaca actttacaaa atgaaagcct ttttcaccag tcttttactg
     8821 ctgaccctta cggcagtgtg gggacaacgc aactgcggca ccatggatgt gctgacagaa
     8881 caactgcagc aaaatccggc gctgcaacaa caaatgcaat ccatcgagca acacaatgac
     8941 cactaccaca accatttgca cgagcaagta ggctttcggg cagtggttac tatcccggtg
     9001 gtttttcata tcattcacaa tggcgacgcc gtaggcagca atgaaaatat ttcggacgcc
     9061 ctgatcctgg cgcagttaca gcagttgaac gacgacttca gaaaactgaa taccgatgcc
     9121 ggcttggtgc ccgccctttt tcaggggctt cacgccgata ccgaaatcca gttctgcctt
     9181 gcccagcgca aacccgacgg cacagccacc aacggcatca atcgggtgca gtacagccag
     9241 gctacctgga cgaccagcca ggtgaacagc accgtgaaac ccgccacaat atggaaccgc
     9301 gaccaatacc tcaatgtgtg gtcggtagca tttggcggca gcagttcggg cattctgggg
     9361 tacgcacagt ttcccggcgg ggctgcttct accgatggcg ttgtcgtggc ttactattcg
     9421 gtaggttcac tggccatgac caaccccggc agcagcacct tcggcaaggg ccgcaccctc
     9481 acccacgagg taggccactg gctcaacctt cgccacatct ggggcgatgc aacctgcggc
     9541 aacgacttgg tggccgatac gccggtacac aacgcctcca acagcggctg cccgacttat
     9601 ccgcacctga gtacctgcac cggcaccccc gtggaaatga ccatgaacta catggactac
     9661 accaacgacg cctgtatgta catgttcacg gcgggccaaa agactcgtat gcaggcagta
     9721 ctcgctaccg gcggcgcgcg tttcagtctc aacagttcgc tgggctgcgt gcctcccggt
     9781 gaaacgaact gcacagcccc cacattagct caattgagcg cttccaatat taccacgtca
     9841 tcggcgcgca tcaattgcag tgtcaccggc gtgaatgcct acgactggcg ctatcgtgtg
     9901 gcaggcacaa gcacctggac cgatctggct tcaggtacac ccaattttgc cgacttgagc
     9961 ggcctggcgg caggtaccac ctacgagttt caggcacagg tgcagtgcgg cagtgtatgg
    10021 agcgcgtggt cggttaccca aacttttaca actctgtcgg ctgcctgcgc cgcgcctacc
    10081 ttggcacagt tgtcggtatc caatatcaca accacctccg tacaattaca atgttccgtg
    10141 agcggcgtca atgcttatga ttggcgttac cggcagcagg gcgcagccac ctggatagac
    10201 ttgccttcta cgactaccgg agtgaccacc gtaaccgggc tttcggcctc caccaattac
    10261 gagtttcagg ctcaggtaca atgcggcagc acctggagcg cctggtccgt gtcgcgatca
    10321 ttcagcacga ccgacggcag ttgcacaacg cctttggctt ctcaaatttc tataacagaa
    10381 atcaccggca gcagtgcgcg tttcaacgtc agcgttaccg gcgccaacag ctacctgtgg
    10441 cggtaccggc aggcgggcgc ctcaacgtgg ataacgctgc ctgctactac cgtcaattat
    10501 acctatgtct tcggactttc gtcaagaacc acctacgaag tacaggttgc attggtctgc
    10561 aattccatta ccacagcctg gtcgggaact gtaaactttt ctaccagcgc aggcggagat
    10621 cctgtttgca atgcgcccga tgtgtcgcaa ttgtctgcaa gcaatgttac tacctccagc
    10681 gctgtcctca actgcacggc tcccggcgcc acctccttca cttggcgtta ccgggtagca
    10741 ggagccaata cctggaacaa cttaggcact acaacgcaag gcacctttgc attatcgggg
    10801 ctttcgccaa atacgactta cgagtttcag gtgatgctgg aatgcagtaa cggcgttgcg
    10861 tcttcctggt cggcatctca gagcttcacc acgccgggag cagccacctg taatgcgccg
    10921 acagcagcac aaatctcggc taccaacatc agcaccacga gtgcgcaact caactgctcc
    10981 atcgccggtc aatcttccta cgcatggcgg tatcgcttag ccggcgcagc tacctggaca
    11041 acgttgccct ctacctccgt caactctacg gtggtgaatg gcctgagcgc ctcttccaac
    11101 tacgaatttc aggtttccat acaatgtacc aacggtgtct ggtcagactg gtcgggcaca
    11161 caaaccttca gcacagccgc cgctgcctgc aatgcgcctt ctgtggcaca ggtatcggtc
    11221 tctaatatta ccgatgcttc ggctcgcctg aactgctctg taaccggtgt taccgcctat
    11281 ctgtggcgtt accggcaggc cggcgcttcc gcctggattg atctgggcag cacaagtgtg
    11341 aacttttacg atctcggcaa tttgtcctct tccaccacat acgaatatgc cgtagctgtg
    11401 caatgcggcg ccgctataag tgcctggtcg gtaacgcaaa acttcactac aacggtgatc
    11461 ctgaattgca ccccgcctac cgccagaatg ctctttgtaa caggcattgc gccaacaggg
    11521 gccaccctca attgcagtat gtctgccacc aattatctat ggaggtaccg cccgctgggc
    11581 acttctgtct ggatcaccgc gggcccgaca acggtaggat cactgaccgt atcggggctg
    11641 atgcctcaga caacctacga gtttcaggta gcggtattgt gtggaaccac acagagcgat
    11701 tggtctgatt cgaagacatt taccaccagc ggcggcactc gcatagagcc gaatcatttg
    11761 cttcgtatgg agctttggcc ggtgccggcg cacgagcagt tgttcattcg ctacactact
    11821 cccgaagatg cgacggtagt catcaccatt tcagacatgg ccggcagggt cgttttgaca
    11881 gaaaacaaag gccgtcagac tgccggcgaa cacctccacg aaatgcggtt ggagcaatta
    11941 cccgcaggta cttattccat acaaatacaa accggcaaga acatttgtcg ggagaaattc
    12001 attaggtttg ccgaatgatt ttgcaaccag catctcattc acataccgaa ctgaagacaa
    12061 catgaaaaaa atcggaattc tttacggctg ggagcgttcg ttcccagccg cttttgttga
    12121 gcgcgtcaac tcgaagaaag tagagggtat cattgccgaa cccgtgcaaa tcagtcaggt
    12181 gcggcaggcc gaatccaccg gatactccgt catcgttgac cgcatttcgc aggacgtgcc
    12241 gttttaccgg gccttcctca aaaatgaggc aagtgcgggc gcggcggtca tcaacaatcc
    12301 cttttggtgg agcgccgacg acaagttttt caacaatgcc gtggccgtaa aagtaggcgt
    12361 gccggtgccc aaaaccgtca ttctgccttc cagagatcat cccaccgaca ccaaggcgga
    12421 gtctttttcc aacctctcct tcccactcga ctgggagggc atgtttcaat acatcggctg
    12481 gccggcgttt atgaaacctt ttgccggcgg aggctggaag catgtgtata aattggattc
    12541 ggccaacgac ttctttgcca aattcggcga aaccggccag ttggtcatgc tcctccagga
    12601 agccatcgag ttcgacgctt atttccgggt gtattgcatc ggcgccaaac atgtgcgcat
    12661 catgcactac gagcctcgca acccgcacca cctgcgctat tcggccaatc acaaggtgtc
    12721 ggacgaactg ctcaaaacca tcgaagatta caccatccgc ctcaatgtgg cgctgggcta
    12781 cgacttcaac accgtagaat ttgccatccg cgacggcatt ccgtatgcca ttgacttcgg
    12841 caacccggcc cccgatgccg accgccactc ggtaggcgac gacaactttg agtgggtgct
    12901 ggagcactcg gcacagtatg ccatagagcg cgcactggcc catcaggaag gcggcgacaa
    12961 cctgacctgg ggtacgttcg tgaaaaactc ggctgctgcc aatgcggccc ccgctccgaa
    13021 aaaggcggcg gcgaagaaga aataattaaa cgaacagtcg gggcaactac ggtagggtaa
    13081 gggcaaccac aagggttgcc ccaaccgcga aggctgcccc gactgaattt aatttgaaag
    13141 caatattacc atggagcctg tacgattggc cgtccttgac ctgtacgaca acacccccaa
    13201 cctcggcatg gggcgcatca aagagttggt agaaatgttc ggcaatgcct acgagtacga
    13261 tgtgttcgac gtgcgcggta aggccgaagt acccgacatg agctacgatg tatatatctc
    13321 atccggcgga ccaggcagcc cccatgacgg cgacggcgtg tgggacgaaa aattcttcca
    13381 ctggatggac gaggtgtggg attggaacgc atcgggcgag atgcccaaaa agtatgtgtt
    13441 cttcatttgc cactcttatc aaatggcctg caaacacttc ggcatcggca cggtgagccg
    13501 gcgcagaacg ccctcgttcg gcatctttcc aacacacaaa accgaggccg gcaaaatgga
    13561 ggcgctcttt gacgggctgc ccaatccgtt ttatattgcc gattttcgcg actggcaggt
    13621 ggtggaacct gatatgcgcc ggatagatga attgggcgcg gaaatactgg cattggagaa
    13681 gattcgcccc aatgtgccgc tggagcgggc catcatggcg gtgcgctttt cgcccgaaat
    13741 tttcggcacg caatttcacc cggaggcaga cgccaaaggt atgttgcacc acttttccga
    13801 ccccgaacgc cgaataaaaa tcattgccga tcactctgag gaaaaatact tggatatgat
    13861 tgaacacctc aacgacccgg acaagatcag cctgacgcaa cgcattgtgc tgccgctgtt
    13921 tctgagcaga tcgctgcggg cggcaagagc ggaaaagatg atgccggaat tggtgtaacc
    13981 cattcaacag accgaccaac catgataccc gaactgcgca atcactacaa cgccgccttc
    14041 acgcaggccc gctacgaggc attcattcag gaaatggtgc aacgctacga gcaccggccc
    14101 tctttcaatg tggccgaaac gccggtgttt attcccactg acctccgaga ccgcctcgtg
    14161 taagcctgcg acgacgtgtg ctccctcttt ttgcgccccg atttcaaagc cttgtcggag
    14221 ggcgccctgc aacccggaca ggtggtgccc aacgaatcgg aacactccga gttttttgtg
    14281 attgacttcg gtatctgcca ggatgaaaac ggccatttga taccacaact cgtggaagct
    14341 cagggatttc cgtctttgtt ttcctaccag catgtactgg ccgaactgta caaaaaacat
    14401 ttcgatgcca tacccgcaca ctacacgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgtgg ccgacagcaa gccggaaaac
    14521 gtcgtcttgc tggaaataga acccgaaaaa caaactactg ccattgattt ttatgccacc
    14581 cgcgaaatgc tgggtattcc ttttgtgtgt ctgacaaaac tcaaaaaaga aggccgcacc
    14641 ttatattata ccaatgaaaa cggccgcaaa attgaggtgg agcgcatcta taaccgcatt
    14701 attttcgacg acctcatggc gcgccctgaa ctgcaaaccg agtttcacct caccgatgat
    14761 gtggacgtgc aatgggccgg gcatcccaac tggtttttcc gcatcagcaa gcatacgctg
    14821 ccgctgttcg acagccctta tgtacccaaa tcttactact tggacaagct cagcgccatc
    14881 cccgacgact tggaaaacta cgttctcaaa ccgctgtttt ccttcgccgg catgggtgtc
    14941 atcgtcaatc ctacccgcga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgctcgtc
    15121 aacaacttag tgcgcctcag caagggcatc atgaccggcg tgcgctacaa caaagaccgc
    15181 acttgggtag gcggctccat cggctttttt gagccgtgag tatgaccata atcaccccgc
    15241 cggtttgact tttgtcatgg cgcagtatgt tgtttatcag gtacattgac tgccgaacca
    15301 taaacaaccc gatatgaaac cggaaaagga tatgtttgaa ttcggcaaag aaaaaatgga
    15361 ccaggcgcgt tttttttttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnc aggaagaaca ggccaaagcc gagcgcctcg aagtgctgga
    15541 aaccaaaatg gacgcgctgg ctgaactgct cgtggccgaa cctgccgaca ccattgaagc
    15601 gtacgatcag gccaaagccg cgctgctgca ccacatttac gacgtggaag cagccctcaa
    15661 aaatgcctat gccgaagccg gcgcttcgct tcggcaacgt ttggatgggt tcagaaccgc
    15721 gctcgacggg taccgtattc cgctggcact cagctcgttt gacaaccagc aagacgccct
    15781 ctccaaaaag gaagaactca acgccgtgct ggccgaactg ctgggccact tccacgccga
    15841 agagaaaaat gaggacaagt tggagcactt catgtctgaa atggaaaccg cctttgagca
    15901 tgtcaaaaaa gctgtaaaag agttgattaa gtaattttga ccaacaacgc caaacataaa
    15961 aacagccctt cgctcctcgc aaggtatgag aaggtgattt ctgtgctgct caagtacggg
    16021 tttgaagacg tggccgctca tccgccgttc aatcgttttt tgcccaaact caacaaaatc
    16081 atacccctgc gcgacggcaa gccggtctat gagtacaccc gctacgaacg tatccgcatg
    16141 gtgtgcgaag agttgggcac cacttttatc aagtttgcac aaattgnnnn nnnnnnaacc
    16201 gacccgacgt cctgcctgaa gccctgctct cggaattgga gcattttcag gatcgcgtgc
    16261 cgttcgtgcc ctcgcaggac atcgtgtcgg tcattcagga agacctcggc aaactgcccg
    16321 aagagctttt cgagcacttc gacaaccaac cgcttgcctc ggcttccatc gcgcaggtac
    16381 accgcgcccg gctgattggg ggcaaagagg tagtgctcaa aatcatgcgc cccggcatag
    16441 acgtcaccat cgaagaagac atccacatcc tcaaacaatt ggccggcctc atggaggctt
    16501 atttcccgca gtaccaggct ttccagccga tggagttggt caaaatgttt gagcgcagta
    16561 ttcgcaaaga actgaaattc agcatagaag ccaacaattt gcgccgcttc gcccagcagt
    16621 tccgcgggca tgaaggcatt tatgtgcccg atctgtaccc ggagttttgt tccgaccgca
    16681 tcctgtgcat ggagttcata gacggcgtca aaatcaccga tccggaggct atttcccgtt
    16741 gcggtttcac cggccccgaa ctggcgctgg caggcatcaa tctgtatttc gagcaggtgt
    16801 ttacgcatgg cttttttcat gccgatccgc accccggcaa catctttgtg ctgcccgaac
    16861 gccagatatg cttcattgat tacggcatga tgggctccat tgtggacagc gaccgcgaca
    16921 tgctggccga cctgctgctc tccgtttcca accgcgacgt ggacggcctc aaagccgccc
    16981 tgctccgttt cacgcccgac ggcacggtgg aaaatgaaaa ggaattggag tacgacatca
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    17101 tcattgaggg tctcaacgcg atgttttttg cttacaaaat caaaatcccc ggcaacctgc
    17161 tgctcctgct caaagccttg gtcattattg aaggcgtggg cctctccctc gatccgcact
    17221 acaacatcat tgccaacata gacccctttg tacgcaaact cctgagccac aaatacagtc
    17281 ccgacaaact gacccgaaaa gccatcagca ccctggccgg catgacgcaa atggccgcca
    17341 acctgcccga agatatcgaa aacatcatcc gcaaaatccg ggagggcaag ctctgcatcg
    17401 aaatggagca caagggcttg gacgacttct accgcaagat ggacgaggta tctaatcgca
    17461 tggccatcag tattttattg gctgcgctca tactcggctc ttcgctgctc gtactggcag
    17521 aggtgccgcc tttcgtgggc aatattccgg cgctgggctt tgtgggtttt gttatttcgg
    17581 ggttgctggc gctgcgcttg gtggtttcgg tgtggaagca tgggaagttt tgagtaggga
    17641 gaggcacatc gccgctgctc ccttcaactg ctatgtttgc ggcaatagat acgcatcatc
    17701 agatgggttc gtttatcgtc cggattgatt ttcagggcca tctcaaaata tcggcacgcg
    17761 gcagtatcat tgtttcgctc caattcaatc tgccccaatc caacataaga ttcatatcta
    17821 tcgctgtcaa tagcaatggc tgtctggaag tcgcgtacag ccgcatcaat atcgcccgca
    17881 gcatagtgga ggtggcctct gtcatgaaat cccaagctga tggaatcaat ggacaatccg
    17941 agattgatat atttcatggc attttcaagc tcgttttgct ccatgtaaca tttggcctta
    18001 aagatatacg ccactgcctc attgaggtct aagctgatgg cctcgttcac gtgttgatgc
    18061 gcttcttcta tcctgccgag ccgatacaac gcagccccgc ctgccgacat gactttgctg
    18121 tttttgggat agcgggaatt gtacatttcc agatattcta atagtttggg atcgtttttg
    18181 tacttggtcg ccgacatatc cgacaacact tgaatgatat tgaaataccc taatcggtag
    18241 tcgttgcaca aagcgagcgc ttgatttatc agttcgtaca catctttcag ttcctcatca
    18301 gagcagcctg cctgttgaca gagaaaatat tgatatgccg ccagataact tactgctaca
    18361 tagttgtttg aatcccgctt gatttcttcc ttcacaaaag aaacagcctc cgaaacgtta
    18421 ccttctttgt aaagctgttc atgctctgca ttgatgcgcg agtgcttttc atccaaataa
    18481 cggtggcaaa tatcttcttg gccggagcac ccggagaatg cgactgcaat agcaaataag
    18541 aggagtactg tggcgtatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn natcaatagc cggcactttc aggaaagaat catatttttg
    18661 caaaaaaaga gatatgccga cagcacttca agaagcgggg gcattgttgc caaaaacatg
    18721 gaattactat cgtgccaatc aggaggagat agacaatttg attgttgaaa atgaacgtga
    18781 attattctga aaactgctaa atttgctcat caaaaaattt aaaactctgt tcactatgga
    18841 aaatcaaaac attccccaag agggcaatga cgaaattgtc aaacaaatct acggatacgc
    18901 agtagaactg ctggtcaacc aacaaaaacc cgcttacgaa gtgcgcaatg ctctgataca
    18961 aagaggcttg gatgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaaaacg
    19021 tcgaaactga agcttccaag gccaagcaat ctcaagccga taaagacatt ctttttgggt
    19081 ctttgtggct ggcaggcggc accatagtca cggttgtttc tatgtctagt gcttccggcg
    19141 gcggtagata catcatcact tggggcgcta ttttgtttgg agccatacaa ctgattaagg
    19201 gccttgttaa taagttttcc tgaagcctac ccctcccgct tcaaatactc tgccaccat
//
LOCUS       DAORLF010000032        19237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1525_length_19237_cov_2.563914, whole genome shotgun sequence.
ACCESSION   DAORLF010000032 DAORLF010000000
VERSION     DAORLF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19237
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1525_length_19237_cov_2.563914"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PK198_04705"
     CDS             <1..1476
                     /locus_tag="PK198_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF38067.1"
                     /translation="GTEEAAADEKTGEYLAKLGIRWPDIENMLARHIKDGLQRKSHLL
                     KGYQKVNPPPIAKFTYKCRNTQTGAAECTEPCRVDFKSESTNVNSSTKYFWNGVELGT
                     KIMFSDTLLKEGSYTMTLEVVNSDGQRNTSTTQTIQINKASLKESVGQYVQPAPPNDS
                     ANSYITLAQKPVAAFRIEKKGCPAPCKITITNESSNINTPHAKIKWYINNRPAGLGAN
                     KIFTHKITSGGEYQIRLEIDNGNGETDTASETVKIKDKKIFSFELQPYISLPIGLYQD
                     VNIFPSWRGDWNPEAGMANFGYGGEMAIGLNVLKFMSLRLQGGYVHNDYNTTALFFQL
                     EDYELQTGQSLQEKFIESTGYRKAYYGASISIEPIPVESTFYLSLEPAYGQIIAPWEN
                     STSVYYTKDDGQRWQTRLVYGLPENGASSYWSGKIQMGVRWGEEDGQRPNLISLSGSF
                     FYSNFATGVQSHSLGDNSQPLVITQSRIQAFHVGIGYRHWF"
     gene            1500..2216
                     /locus_tag="PK198_04710"
     CDS             1500..2216
                     /locus_tag="PK198_04710"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF38068.1"
                     /translation="MKLHKLLQIAFFIGILGMMTSCQQETPRPIADFIIINDNCGAPC
                     TVRFTNTSSTEGDAKYLWRFGEGSPLDTAFGNVQHRYLNPGTYQVSLIAESAGGVSEK
                     TAFVTIGCNATPGLLSLTLQDYPRFKPSGEPWDRLSCCPDIEMRIIDVDRNVTVITGN
                     AVTNVNMPPVFWSSNSPVSFLGATRIKIQFWDIDNPEPAELMGETNVISLHGENICRV
                     QNISLTGSNFVKVALQLQWN"
     gene            complement(2573..3580)
                     /locus_tag="PK198_04715"
     CDS             complement(2573..3580)
                     /locus_tag="PK198_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF38069.1"
                     /translation="MLPEHLKFPLLIRLVDKQLYVIDPLNNADKVHIKDEILSINGVA
                     LEQLIADIYPHITAQGYIQTTKRHVFNMWSNILIPYALGFPNTYTIVLKGQAAPITLK
                     KAAGGIRLRRNPIFYCPDGLCLEFLDDQKTAVMSITSFNYYPFNDLDVFVAFVDSSMA
                     KIKRKGVKNLIIDVRWNGGGSQQSSIHLLRYLASKPFLYYSKAEFAGKTGKIDGEEVF
                     EPYTDRYKGKVYFMMDGLGNSTTGHFMSLAKVLNLGTIVGEELGSNQFCSAGQTVCRL
                     KYTKLQYYVANNTHVSSATSLPDERGILPDHYISQSIDDYLNGIDRVKDFTIQLTKQQ
                     K"
     gene            complement(3641..4492)
                     /locus_tag="PK198_04720"
     CDS             complement(3641..4492)
                     /locus_tag="PK198_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38070.1"
                     /translation="MAKALFSKNLPQTGMAIVHIFTGWFMFLFAVFFINGAGKWSMDN
                     WLRNVFFSAKAGAAFNGLMMLLCLSSCGLNKSPHPTTEQTASKETEMPKQSLVQKLKE
                     LEHLPVAERIALYRQLKTESPDAYDFANEDELTMYGYMMLWNNNVKDAIEIFKLIVQE
                     FPHSANAYDSLGEGYLNAGDTVRSLANYEKSLAMNPDNFNAEDQIERIKYPDKPIETP
                     ADKFAKVFSAAEYRADLDQLGATLLKVHPNALKFISEQDFRNLIERKKALITDNTTYA
                     AFAWHCS"
     gene            complement(4586..5146)
                     /locus_tag="PK198_04725"
     CDS             complement(4586..5146)
                     /locus_tag="PK198_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF38071.1"
                     /translation="MPAVIAKKRFTVADYHKMAEAGVLPERGVELINGEIIEMSPVGS
                     KHLFCVNALTELLTLKLAGKFIVSVQNPVRLSNFSEPEPDLAVLQRVDNRYADRLPAA
                     KDALLLIEVADSSYVYDREVKLPAYAQSGIPECWIVNLEAQELEVFWLPVGDAYQHNK
                     VWRAGDVVRAQYFELELAVGEILPGS"
     gene            complement(5195..5845)
                     /locus_tag="PK198_04730"
     CDS             complement(5195..5845)
                     /locus_tag="PK198_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HRF38072.1"
                     /translation="MKRISVLLLLVQLLSAGALPAQNAWKLQKEKDGIKVFFRQAEDS
                     NLKELKINTRMQGSLGAAVAFISDIDNFPNWMDHCREVKYIDKRSPSDYTYYNLADFP
                     WPLSDREFVMRCTSWQDPTSLACYFQSVADPKAIPPNKNYVRVQLTVSQWKIEPDGPG
                     FIKLEYYLKADPGGAIPAWMVNAALDNGPVKAMLALRNAVQHEPYAGRKLSYVREP"
     gene            complement(5926..6357)
                     /gene="mscL"
                     /locus_tag="PK198_04735"
     CDS             complement(5926..6357)
                     /gene="mscL"
                     /locus_tag="PK198_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRF38073.1"
                     /translation="MLKEFKEFAIKGNMLDMAVGIIIGAAFGTVVSSFVGDIIMPIIS
                     SIFSAPDFGNLFVLLKAPADTTGVNMSSIAAVREAGGVALGYGLFINAVISLLIVAMA
                     LFIVVKGMNKLKKEQEVAAPAPAGPTAEDLLAEIRDLLKKQ"
     gene            6501..7580
                     /gene="paaK"
                     /locus_tag="PK198_04740"
     CDS             6501..7580
                     /gene="paaK"
                     /locus_tag="PK198_04740"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRF38074.1"
                     /translation="MSTPKFHALKIREIRRETADCVSVSFDVPEAVQDAYRFEAGQHL
                     TLRKDFDGEDVRRSYSICVAPSEGELRVAVKELPGGRFSTFVNRTLNVGDVLEVMTPS
                     GKFYHPPASSDAGRHVAAFAAGSGITPVMSIMKTILENEPQSTFKLFYANRKPDSVIF
                     LEEVEDLKNRYLRRLSVHHVLSRQESDSPLFNGRIDADKCRTFCKTLLDVAHTDDFYL
                     CGPAAMVETLRQTLQEEGADPKKIHTELFFAPGQGEQTQRATDETVPAVEAHIGITLD
                     GRSFEFPLASRGDTILDAALQAGANLPFACKGGVCCTCKALLTEGEVRMDVNYGLEPD
                     ELEAGFILTCQSHPLTPLVKVNFDV"
     gene            7684..8259
                     /locus_tag="PK198_04745"
     CDS             7684..8259
                     /locus_tag="PK198_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF38075.1"
                     /translation="MPEPKSKRDEIREAAARLFRDKGYAATSMRDLAGAVDIKASSLY
                     NHFDSKEDILRQICFDNAQRFLAGLQEVENSADSATEKLRALIGLHIQIATEDLTSIT
                     AFNDEWRHLNEPYLSEFKTIRRDYERRFQSIIEDGMNKGELRSMDSHIATYTLFSSMR
                     WLYDWYKSGKRISADELQRDISALLMNGLSV"
     gene            8361..9617
                     /gene="metK"
                     /locus_tag="PK198_04750"
     CDS             8361..9617
                     /gene="metK"
                     /locus_tag="PK198_04750"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRF38076.1"
                     /translation="MPYLFTSESVSEGHPDKIADQISDALVDHFIAFDPSSKVAVETL
                     VTTGQVVLAGEVRSEVYLDVQQIARDVIKRIGYTRSEYMFEAHSCGVLSAIHEQSADI
                     ARGVERANPEDQGAGDQGMMFGYASNETENYMPLALDLAHRILRELAAIRHEGKEMTY
                     LRPDSKSQVTIEYQDNHQPSRIDTIVVSTQHDDFDADETMLAKIREDVINIVIPRVKA
                     KLSPHIQQLFNDLIIYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSGAYATRHIAKNLVAAGVCDEVLVQVSYAIGVAKPTNIYVQTYGTAKVNM
                     SDGEIAQKVNELFDMRPYAIEKRLQLRTPIYSESAAYGHMGRNPERKTLEFFSANGER
                     KQIEVETFTWEKLDMVDAIRGAFGLE"
     gene            9791..>10044
                     /locus_tag="PK198_04755"
     CDS             9791..>10044
                     /locus_tag="PK198_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF38077.1"
                     /translation="MKTFATFVLALVASFSSTFLHAQDLNSKIDQLLSAQFPADGPGC
                     TAIVAKGDQVLYRKAFGMAHLELDVPMKPEHVFRIGSITK"
     assembly_gap    10045..10071
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10072..11434
                     /locus_tag="PK198_04760"
     CDS             <10072..11434
                     /locus_tag="PK198_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF38078.1"
                     /translation="LEEAGKLSVQDDITKYLPDYPTQGHKITIEHLLTHTSGIRSYTD
                     MPEFGDVMRTDQSPEDFIAFFKDQPMDFAPSEKWQYNNSGYFLLGVIIEKVSGMPYDK
                     YIEETFFKPLGMSHSHYGHNAPVFKNRVAGYTLSNDGVVNCDLLSMDLPYAAGSLLSN
                     VDNLYTWYRAVMSGKVLSKKSMEKAHTAYKLNDGKATQYGYGWFLGDVQGSPTIEHGG
                     GINGFITASIYLPKEQVFVAVFSNNEGNSPDVAARKIAGLAIGKPYEWTKILMPEQEL
                     AQYAGNFESEDGSQMRSITFENGQLFSQRSGGTKFKIYPFAKDKFFFDESTTTLEFNR
                     AGGKIESVTAHAVSGKETWRKTDKKPTTRTEIQLDQATLQQYVGKYEVQPSFAFTFTL
                     EDGQLMTQATGQPKFPVFAEKKDFFFLKVVEAQIEFFRNDAGVVERLVLYQGGQKIPA
                     LKK"
     gene            11678..13117
                     /locus_tag="PK198_04765"
     CDS             11678..13117
                     /locus_tag="PK198_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF38079.1"
                     /translation="MFRAFSIAAAAFFLSIFTHSLHAQSPDSARVFTQEELFEWLLLY
                     HPVARQANLLDDEARAQLRMARGFFDPKLYSEWEQKSFDGSEYFTFGNSGLKVPTWYG
                     LELKAAFNTATGIQVDPEHKLPAAGQAVLGINATLGRGMMIDERRAALQQARIFEQSN
                     EAQRRLVVNDLLMDAVKAYWEWTAAYHQREAILQALELARVRQQGIVEAYRQGDKPAI
                     DTLEAFIQVQTRQADLNDANVSYLNTGQMLSNFLWWENDTPLELTGRLRPAPLDESVG
                     SAVLPSVEELAQQAAENHPILKFYFLKINTLEVDRRLAAEQLKPQLDVEYNLLGNGLN
                     LAYDANGGGSSFNNLFFQNYKWGLQFNMPIFLRKERAKLELTRLKIQDSGLELQQKRL
                     EVANKVRAYFNEWQNTQQQVSLYQSMQTNYRALLDAEITRFQLGESSIFLINAREQKL
                     VEVQLKLLKLLAEFQKQRMYTEWAVGRLG"
     gene            13276..14010
                     /locus_tag="PK198_04770"
     CDS             13276..14010
                     /locus_tag="PK198_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRF38080.1"
                     /translation="MIRDDRRQLNERFIRVFRLLEERGDIVKNDRGGKGIGDFAEKIL
                     GNRAYGHIVRAYLNEDDKRCIDYQQARIICREYGINESYLIDGIGTPFGFDLPAPRYQ
                     EPTPSARGNILFTTVEAFAGTAVDVGGFAAEDLEFFSIPGLSGGGLVSFAIKGNSMEP
                     VISNGDIVICREVAGAHEIKDNRIYAIKNNGSLWVKYVQRLPDNRGRTARLKLISANH
                     LEHEPFIEEVNEHTRVYHVIRRVSNL"
     gene            complement(14219..14794)
                     /gene="ruvC"
                     /locus_tag="PK198_04775"
     CDS             complement(14219..14794)
                     /gene="ruvC"
                     /locus_tag="PK198_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRF38081.1"
                     /translation="MNSQTTTHRILGVDPGTNVLGYAILEIHNKTLTVLHLGVIHFKK
                     DEEHPYRLERIFTQLQKIISDYLPREMAIEAPFFGKNVQSMLKLGRAQGVAMAAAITK
                     GVPITEYSPKKIKQVVTGNGNASKEQVSAMLSTTLGVNLQDQILDATDALAAAVCHYY
                     STRNGSAASSAKSGSGWGSFIANNPGRVKKT"
     gene            14901..>15185
                     /locus_tag="PK198_04780"
     CDS             14901..>15185
                     /locus_tag="PK198_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38082.1"
                     /translation="MFAGLLYRHIRTMPVSGNWDALFTWERLEKEGVWLLLAVALMPL
                     NWSLETLKWQRLMCVFGAVSFRRAFAAVLSGVTISLFTPNRIGEYGGRLLA"
     assembly_gap    15186..15219
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15220..15887
                     /locus_tag="PK198_04785"
     CDS             <15220..15887
                     /locus_tag="PK198_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38083.1"
                     /translation="SLLGTWIQWIVLLVGGVLGFYHLAVQYELTETGRAAGLGAAGMA
                     LAAGAVLALFRIPVLAALAQWHLRGQWRRRVLSKLLPLRHYSGSSIGLSLLWAALRYM
                     VYCTQYYLMLRFFGIHIPAGEAAAGIAAIFLAQTSLPLPPLAGLIARGELALWVWGLS
                     GADTASILGAAYGLFIINLSLPSLVGAWILLRLNLRKFWKNERKNMENSALDAAAADS
                     DRL"
     gene            15823..16704
                     /locus_tag="PK198_04790"
     CDS             15823..16704
                     /locus_tag="PK198_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRF38084.1"
                     /translation="MNAKTWKTALWMPLLLILTAFDFPYTTGQSADTANLCETSNSAF
                     KAGEELTYKVYYNWNFVWLSAGEVTFRVRDVNGEYHLSAHGSTYKSYDWFFKVRDKYD
                     TYVDKKTLLPRLSIRDVNEGKYSLYDAVTFDRPAGKATSLRGKTKDIATPTDYTINPC
                     AHDILSILYYARNLNYSKMRSGQTIPVNIFIDKEEWNLKVSYLGKEESKKVRNLGRFR
                     AVKLSPQVIEGYVFKKDARLTIWASDDENRVPLVIESPVSVGSVKVILKSYKNLRYPV
                     TSKVGEDDGVDEDGIKD"
     gene            16759..17367
                     /locus_tag="PK198_04795"
     CDS             16759..17367
                     /locus_tag="PK198_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRF38085.1"
                     /translation="MNKRVAVGMVAVILMVAGFFIGRSLYRQKQEIVAQEQSQVLLER
                     IEHVAKLITVEGYFSEVYDYKDYWRFDWSPFQKKALLRVKAKVSVGYDLTKMKLESRP
                     EEKLLIVSHLPDPEILSTDHDVDYYDLSEGVFNSFTEADYTKMNRNAKKVIEEQARKS
                     DLMLSAEKQAYEILELMRFMVESSGWRMEFQPEGLKQEEKKG"
     gene            complement(17476..17625)
                     /locus_tag="PK198_04800"
     CDS             complement(17476..17625)
                     /locus_tag="PK198_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38086.1"
                     /translation="MNIYFCPTTKDWRIVFTKEFPEKKEAAAFERQIKAWKSRSRMER
                     LIAGA"
     gene            complement(18047..18121)
                     /locus_tag="PK198_04805"
     tRNA            complement(18047..18121)
                     /locus_tag="PK198_04805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18084..18086),aa:Val,seq:tac)
     gene            complement(18137..18214)
                     /locus_tag="PK198_04810"
     tRNA            complement(18137..18214)
                     /locus_tag="PK198_04810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18177..18179),aa:Val,seq:cac)
     assembly_gap    18505..18632
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18633..>18881)
                     /locus_tag="PK198_04815"
     CDS             complement(<18633..>18881)
                     /locus_tag="PK198_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38087.1"
                     /translation="GRLFGHYFIQLMPVFALLAGEFFAAGRSDALPRWLRPLLQRRLG
                     YPLLALLMLANVLLQKKDYFDKPDYPRIVSNWLRERMQ"
     assembly_gap    18882..19023
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19024..>19237)
                     /locus_tag="PK198_04820"
     CDS             complement(<19024..>19237)
                     /locus_tag="PK198_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38088.1"
                     /translation="GLFLWWRQAQFKSGWPVFWLRALLMMVAMLLPFALAWAYYAQAG
                     LGDTFRFYTFEVMKNYPVSRSLPDYLK"
BASE COUNT         4898 a         4772 c         4695 g         4541 t
ORIGIN      
        1 ggaacggaag aagcggcagc cgacgaaaaa accggtgagt atttggccaa actgggcatc
       61 cgatggccgg atattgaaaa catgctggcc cggcatatca aagatggact gcaaagaaag
      121 agtcacttgc tcaaagggta ccagaaagtg aacccaccgc ctattgccaa gtttacatac
      181 aaatgcagaa acactcaaac gggcgcggcc gagtgtactg agccttgtcg tgttgatttc
      241 aaaagcgaat caacaaatgt gaatagttca actaaatact tttggaatgg agtagaatta
      301 ggaactaaga taatgttttc cgatacatta ttaaaagagg gatcgtacac tatgacctta
      361 gaagtcgtca attccgacgg gcagagaaac acctctacta cacaaaccat tcagataaat
      421 aaggcttctc ttaaggaaag tgtcgggcaa tatgtgcaac cggctccgcc taacgacagt
      481 gcaaattctt atatcactct agctcaaaag cctgttgctg catttagaat tgaaaaaaag
      541 gggtgccccg caccttgtaa gattacgata acaaacgaat ccagcaatat caatacccca
      601 cacgccaaga taaagtggta tataaataat cgtcctgcgg gcttgggggc caacaaaatt
      661 tttactcaca aaattacaag cggaggagaa tatcagatca ggttggaaat agataatggc
      721 aatggagaaa ctgatacagc ttctgagact gtaaaaataa aggataaaaa aatattttca
      781 ttcgaacttc aaccttatat aagtcttccc atcggactct atcaggatgt aaatattttc
      841 ccatcctgga ggggcgactg gaacccggag gcaggcatgg ccaactttgg ctacggagga
      901 gaaatggcca ttgggctaaa tgtattgaaa ttcatgagcc tgcggcttca gggcggctat
      961 gttcacaatg attacaatac gacggcattg ttttttcaat tggaggatta cgaactccaa
     1021 accggtcaat cgctgcaaga aaagtttatt gagagcaccg gataccggaa ggcttattat
     1081 ggggcatcca tatctattga accgatacca gtggaaagta ctttttatct ttccttagaa
     1141 ccggcttatg ggcaaatcat tgccccctgg gaaaactcca cttccgttta ttacacaaaa
     1201 gatgacggac agcgctggca aacccggttg gtgtatggtt tgcctgaaaa tggagcgtct
     1261 tcgtattggt caggaaaaat acaaatgggg gttcgttggg gagaagaaga tggccaacgc
     1321 cctaacctta tctctctttc cggcagtttt ttttattcca atttcgctac cggagttcaa
     1381 agtcattctt tgggagacaa ttcccagcca ttggtgataa cgcaatcccg tattcaggca
     1441 ttccatgtag gaattggata caggcattgg ttttgaaaaa atgaaaaaac ttaattgaga
     1501 tgaaactgca taagcttctt caaatagctt tttttatcgg catcttaggc atgatgacct
     1561 cttgtcaaca agaaacgccg cgccccattg ctgattttat catcatcaat gataattgtg
     1621 gcgctccctg caccgtcagg tttaccaata cctccagcac agaaggtgat gccaaatatc
     1681 tatggcgttt cggtgagggc agccctttag acactgcttt tggcaacgta caacaccgct
     1741 acctgaatcc cggaacgtat caggtcagcc tcatcgctga gagcgccggc ggtgtgagcg
     1801 aaaaaaccgc atttgtgacc atagggtgca atgccactcc cggccttctg tcgttgacac
     1861 tacaggatta tccaagattt aagccatcag gggaaccgtg ggatcggcta agctgctgcc
     1921 cggatattga aatgcggatc atagacgtag atcggaatgt aaccgtaata acaggtaacg
     1981 cggtaacaaa tgttaacatg cctcctgttt tttggagttc caattctccc gtttcttttc
     2041 tgggggctac gaggatcaag attcaatttt gggatattga caaccctgaa ccggcagagt
     2101 taatggggga aacaaacgtc atttctcttc atggagaaaa tatttgcagg gtacaaaata
     2161 tatctcttac cggaagcaat tttgttaaag tggcgcttca attgcagtgg aactgacaag
     2221 gcggaagaac aaacaaaaag aaagtagatt tcaggaagat tgccatattg ggggtaatga
     2281 cggcagacat agatatactg ctttatggta atatgtcctg tactaccaga tcaagatact
     2341 gcgcccgcac cacagctccg gcgcgccata cctcgttgtg ctgataggca ccgcccacag
     2401 gcaaccagaa gacttccagc tcttgcgctt cgaggttggc aatccaatgt tcgggtatgc
     2461 tgtttgggga ataaacagca acagaagcga atttgctttg ccctgctact aattgaatac
     2521 agcgcaatga aaaaagccgc atctgccaca aaaaccaagg taagccatcg tttcactttt
     2581 gttgtttcgt caattgaatc gtgaaatctt ttacccggtc aatgccattt aaatagtcat
     2641 ctatgctctg cgaaatgtaa tgatcgggca gaatccccct ttcatcgggc aatgaagtgg
     2701 cggatgacac atgggtgtta ttggctacat aatattgcag tttggtgtac ttgagcctgc
     2761 agaccgtttg tccggccgag caaaactgat tggaacctaa ttcttcaccg acaatcgtgc
     2821 cgaggttaag caccttggcc agagacataa aatgcccggt ggtagaattg cccaagccat
     2881 ccatcataaa atagaccttg cctttgtatc tgtctgtgta aggttcaaaa acctcttccc
     2941 catctatttt gcctgttttt ccggcaaact cggctttgga ataatacaaa aagggctttg
     3001 aggccaaata ccgcaacagg tggatgctcg actgctgaga gccgccgccg ttccaacgaa
     3061 cgtcaataat cagatttttg acgcccttgc gcttgatttt ggccatgctg ctgtccacaa
     3121 atgccacaaa aacatccaag tcgttaaacg ggtaataatt gaacgaggta atgctcatga
     3181 cggcggtttt ttgatcgtct aaaaattcaa gacacaagcc gtccgggcaa taaaaaatgg
     3241 gatttcttcg cagacggata ccgccggctg cctttttcaa tgtgatgggt gcggcctgcc
     3301 ccttgagtac aatcgtatag gtgtttggaa agccgagcgc gtaaggaatg aggatattgg
     3361 accacatatt aaacacatgc cgtttggtgg tttgtatata accctgcgct gtaatatgcg
     3421 ggtaaatatc agcgatcaat tgttccaaag ctactccatt gatgctcaat atctcgtctt
     3481 tgatatggac tttgtccgca ttgttcaacg ggtcaataac gtacaactgc ttgtctacca
     3541 agcggataag cagcggaaac ttcaggtgtt ccggcaacat ttcattctca atattaaacc
     3601 tgcccatctc ggtatgcgag caataaacgc ttgcaatgat ctagctgcaa tgccaggcaa
     3661 acgctgcata agttgtatta tcggttatca gcgctttttt ccgctctatc aagttgcgaa
     3721 aatcctgttc cgaaatgaat ttgagcgcat tggggtgaac tttcagtaaa gtggccccca
     3781 gttgatctaa gtctgcgcgg tattcggcag ccgaaaacac tttagcaaac ttgtcggcgg
     3841 gagtttcaat gggcttatcc gggtatttga tgcgttcaat ctggtcttcg gcattaaaat
     3901 tgtcggggtt catggccagc gacttttcgt agttggccag cgagcgcacg gtgtcgcctg
     3961 cattcagata gccttctccc aaactatcgt aggcgttggc agaatgcgga aactcctgca
     4021 caatcagttt gaatatttcg atggcatcct tcacattgtt gttccacaac atcatgtagc
     4081 cgtacatagt gagttcatcc tcattggcaa aatcgtatgc gtcggggctt tccgttttca
     4141 actgcctgta cagggctatg cgctcggcca ccggcaagtg ctccaattct tttagttttt
     4201 gaacaaggga ttgtttaggc atctcagttt ctttgctcgc ggtctgttcg gtagtagggt
     4261 ggggcgattt gttaagcccg caggaactca ggcaaagcag catcatcaga ccattgaagg
     4321 cagcgccggc tttagcgctg aagaacacat tcctaagcca attatccatg ctccatttcc
     4381 cggcgccatt gataaaaaag accgcaaata agaacatgaa ccaacccgtg aaaatgtgga
     4441 cgatagccat acctgtctgc ggcagatttt ttgaaaataa tgcttttgcc attttatcat
     4501 tttttgtgtg gcaaaagtgc cgctgatggg gctatgaagt attcaatgag gtgtaaaaaa
     4561 gtgtttcata agtgtaaaac cttcatcagc tccccggcaa aatctctccg accgccaact
     4621 ccaactcgaa atactgcgcc cgcaccacat ctccggcacg ccataccttg ttgtgctgat
     4681 aggcgtcgcc cacaggcaac cagaagactt ccagctcctg cgcttcgagg ttgacgatcc
     4741 agcattcggg tatgccgctt tgggcgtaag ccggcagctt tacttcccgg tcatagacat
     4801 aagaactatc ggcaacctca atgagcaaca aggcatcttt ggcggcgggc agtcggtctg
     4861 catagcgatt gtccactcgt tgaagcacgg ccagatcggg ttccggctct gagaagttgc
     4921 tcaacctcac agggttctgt acgcttacta tgaatttccc tgccagcttc agggtcagca
     4981 attccgtcag ggcattgacg caaaagaggt gtttacttcc tacgggactc atttctataa
     5041 tttctccatt gatgagttcc acgccgcgtt cgggcaatac gcctgcttcg gccatcttgt
     5101 gatagtcggc tacggtgaag cgttttttgg caatgacggc tggcatgggc gcgaactttg
     5161 atgacaaagt taagtaaaaa tctttgttat tccatcacgg ctccctcaca tagctcagtt
     5221 tcctaccggc ataaggctcg tgttgcactg catttcgcaa tgccagcatg gccttcaccg
     5281 gcccgttatc taatgcagca ttcaccatcc aggcgggtat ggcgccgccg gggtcggctt
     5341 tgaggtagta ttccaacttg atgaatccgg ggccgtcggg ttctattttc cattgtgaga
     5401 cagtgagttg cacccgcaca tagtttttgt tgggaggaat ggctttgggg tcggcaaccg
     5461 actgaaaata acaggccagc gaagtggggt cttgccagga ggtgcagcgc atgacgaact
     5521 cgcgatcact cagcggccag ggaaaatcgg ccagattgta gtaggtataa tcggagggcg
     5581 agcgcttgtc aatgtatttc acttcccggc agtggtccat ccaattgggg aagttgtcaa
     5641 tatcactgat gaaagcgacc gcagcgccca aagacccctg catacgggta ttgattttca
     5701 actctttgag gttggagtct tccgcctgtc ggaaaaagac tttgatgccg tctttttcct
     5761 tttggagttt ccaagcgttc tgggccggca aggcgccggc agataaaagc tgtacgagca
     5821 gtaataaaac agagatgcgt ttcacgtttt tgggaatttg cacaaagata atcggctata
     5881 tccaaaatcg caaaacgcat cttgtttgaa aaaagtcttt acctattatt gctttttcag
     5941 caaatcacgg atttcagcga gcaggtcttc tgccgtaggg ccggcagggg ccggagcggc
     6001 aacttcctgc tcttttttga gtttgttcat gcctttcacc acgatgaaca aagccatagc
     6061 cacgatgagc agagagataa ccgcgttgat gaacaagccg taacccagag ccacaccgcc
     6121 tgcttcgcgc accgccgcga tggaactcat gttcacgcct gtcgtatcgg ccggagcttt
     6181 gagcaagacg aacaggttgc cgaagtccgg cgcactgaaa atagaggaga tgatcggcat
     6241 gatgatgtcg cccacaaatg agctgactac cgtaccgaaa gctgcgccga tgatgatacc
     6301 gacggccatg tcgagcatgt tgcctttgat ggcaaactcc ttgaattctt ttaacatagt
     6361 tttccgtttt tgatgtaaaa aattgataaa gcgccctttg gacggtggaa aaatagaaag
     6421 ttacttctaa atcggcaaga aagagataaa aaaatgtaaa aactcatacc tttgccaact
     6481 aacgttcatt cgtaatttct atgagtacgc ccaaattcca cgccctgaaa atccgcgaaa
     6541 tcagacggga aaccgccgac tgtgtttccg tttccttcga tgtgcccgaa gcggttcaag
     6601 atgcctatcg ttttgaggcc gggcaacatc tgacactccg caaagatttc gacggagaag
     6661 atgtgcgtcg cagttattct atttgcgttg cgccctcgga gggcgagctt cgggtggctg
     6721 tgaaagaatt gccgggcggg cgcttttcta cttttgtcaa ccggacattg aatgtaggcg
     6781 atgtgctgga ggtaatgacg ccttccggca aattttatca tccgccggcc tcgtcagatg
     6841 cgggcaggca tgtagccgcg tttgcagcgg gcagcggcat cacgccggtc atgtccatta
     6901 tgaaaaccat tttggaaaac gagccgcaga gtacttttaa gctgttttac gccaaccgga
     6961 aaccagactc cgtcatcttt ttggaagagg tggaagacct caaaaacaga tacctccgcc
     7021 gtttgagcgt gcatcatgta ttgagccggc aggaatcgga cagcccgctt ttcaatggca
     7081 ggatagatgc ggacaaatgc cgtaccttct gtaaaacctt gttggatgtg gcgcacaccg
     7141 acgactttta cctttgcggc ccggcggcta tggtagaaac cctgcggcaa accttacagg
     7201 aagagggcgc cgatcctaaa aaaattcata ccgaactttt ctttgcaccc ggtcagggag
     7261 aacagacaca gcgagccact gatgaaaccg ttcccgctgt tgaggcccat atcggcatca
     7321 ccttagatgg tcgcagcttc gagtttccgc tcgcttctcg cggcgacacc attctggatg
     7381 cagcccttca agcgggcgcc aatctgcctt ttgcctgcaa aggcggcgta tgctgtacct
     7441 gcaaagccct gctgacggag ggcgaggtgc gtatggatgt caattatggc ctggagccag
     7501 acgaactgga ggccggcttt atcctcacct gccagtcgca cccgcttacg cctttggtaa
     7561 aagtcaattt tgacgtgtga atgtttaaag tttagtgaag gggtgactca tccgaggctg
     7621 taaatcagta gtcacccctt cactacgcat attcttccca cccaaacaca acacaccatg
     7681 actatgcctg aaccaaaatc caagcgcgac gaaatccggg aagcggcggc gcgccttttt
     7741 cgcgacaaag gatacgcggc cacttccatg cgcgatctgg ccggcgctgt tgatatcaaa
     7801 gcttccagtc tttacaatca tttcgattct aaagaagaca tcctgcggca aatctgtttc
     7861 gacaatgccc agcgcttttt ggcgggtttg caggaagtag aaaactcggc agattcggcc
     7921 accgaaaagc tgcgggcatt gatcggcctg cacatccaaa tagctaccga agacctcaca
     7981 tccatcacgg ctttcaatga tgaatggagg cacctcaacg agccgtatct gagcgagttc
     8041 aaaaccattc ggcgagatta cgagcgccgg tttcagtcca ttatcgagga tggcatgaac
     8101 aaaggagaat tgcgttcgat ggattcccat attgccacat acaccctttt ttcttccatg
     8161 cgttggctgt atgactggta caaaagcggc aagcgaattt ctgccgacga gctccaacgc
     8221 gacatcagtg cattgctgat gaacggattg tcggtgtaat tttgccctgc ctttttcagg
     8281 attggaccgc gctatatgta aggaatggct tacctttgcc acgctttttg aaaagcgctt
     8341 cattaaaagt ttgactcaca atgccatatc ttttcacttc cgagtcggtt tccgagggac
     8401 accccgacaa aattgccgac caaatctccg acgcattagt tgaccacttc attgccttcg
     8461 acccctcctc caaagtggcg gttgaaacat tggtaactac cggacaggtg gttttggcgg
     8521 gcgaagtgcg ctccgaagtt tatctcgacg tgcagcaaat tgcccgcgac gtcatcaaac
     8581 gcatcggtta tacccgctcc gaatacatgt tcgaggccca ttcctgcggc gttttatcgg
     8641 ccatccacga gcaatcggcc gacatagccc gcggcgtaga acgcgccaac ccggaagacc
     8701 agggcgccgg cgatcagggt atgatgtttg gctatgccag caacgaaacc gaaaactaca
     8761 tgccgctggc cctcgatctg gcgcatcgca tcctccgcga attggctgcc attcgccacg
     8821 aaggcaagga aatgacctac ctccgccccg attccaaatc gcaggtgacc attgagtacc
     8881 aggacaacca ccagcccagc cgcattgata cgattgtggt gtccacgcag cacgacgact
     8941 tcgatgccga tgagacgatg ctggccaaaa tccgggaaga cgtcatcaac atcgtcattc
     9001 ccagagtgaa ggccaaatta agcccgcaca ttcagcagct tttcaacgat ctgatcattt
     9061 atcacatcaa ccccaccggc aagtttgtca tcggcggtcc gcacggcgat accggcctca
     9121 ccggtcgcaa aatcatcgtg gacacctacg gcggcaaagg cgcgcatggc ggcggcgcat
     9181 tttcgggtaa agacccctcc aaggtggacc gctccggggc ttatgctacc cgccatattg
     9241 ccaaaaactt agtggccgca ggcgtttgcg atgaggtgct ggtgcaggtt tcgtatgcca
     9301 tcggcgtggc caagccaacc aatatttatg tgcaaaccta cggcacggcc aaggtgaata
     9361 tgtcggacgg cgagatcgcg caaaaagtga acgaactgtt cgatatgcgt ccttatgcca
     9421 ttgagaaacg cctgcaactg cgcacgccca tctattcgga aagcgccgct tatggccaca
     9481 tgggccgcaa tccggaacgc aaaacgctgg agtttttcag cgccaacggc gaacgcaaac
     9541 aaatagaagt ggagaccttc acctgggaaa aactcgacat ggtggacgcc atccgcggcg
     9601 cgttcggatt ggagtaaaat atgaaaaagt cagggggctg atttagatag cttcctgact
     9661 tttttcaaaa acatatcccc caaattattc cccgctcccc gaactccggc aagattttcc
     9721 tttgttttcg tcgttttttt tatggtccaa gggccaatgc gccccatact tgcacctcaa
     9781 aaaacacgac atgaaaacct tcgcaacttt cgttctggcg cttgtcgcct ccttttcaag
     9841 caccttcctg catgctcagg atttgaattc caaaattgac caactccttt ccgcgcagtt
     9901 ccccgccgac ggccccggct gcactgccat cgtagccaaa ggcgatcagg tattgtaccg
     9961 caaggcattc ggcatggcgc acttagagtt ggatgtaccc atgaagccgg agcatgtgtt
    10021 tcgcatcggc tccattacca aacannnnnn nnnnnnnnnn nnnnnnnnnn nattggaaga
    10081 agccggcaag ctctctgtgc aagacgacat caccaagtat ctgcccgatt accccacgca
    10141 aggccacaaa attaccatcg aacacttgct cacgcacact tcgggcattc gcagctacac
    10201 cgatatgccg gagtttggcg atgtcatgcg caccgaccaa tcgcccgaag atttcatcgc
    10261 ttttttcaaa gatcaaccga tggatttcgc gcctagcgaa aaatggcagt ataacaactc
    10321 cggttatttc ctgctgggcg tcattattga aaaggtgagc ggaatgcctt acgataagta
    10381 catcgaggaa acttttttca aacccttggg catgagccat tcccactacg gacacaacgc
    10441 tccggtcttc aaaaacagag tggccggcta taccctgagc aatgacggcg tggtgaactg
    10501 cgacctactg agcatggact tgccctacgc agccggctcg ctcctttcca acgtggacaa
    10561 tttatatacc tggtaccggg cggtgatgag cggcaaggtg ttgagtaaaa aaagcatgga
    10621 gaaagcacat actgcctaca aactcaacga cggaaaggcc acgcaatacg gctacggctg
    10681 gtttctggga gatgtgcagg gcagtcctac catcgagcac ggcggcggaa tcaacggctt
    10741 cattacagca tctatctatt tgcctaaaga acaggtattt gtggctgtct tttccaataa
    10801 tgaaggcaat tcccccgacg tggccgcccg aaaaatagca gggctggcca ttggcaaacc
    10861 ttacgagtgg accaaaatct tgatgccgga gcaggaactg gcgcagtatg caggcaattt
    10921 tgaaagcgaa gacggctcac agatgcgcag catcaccttc gaaaacgggc agttgttttc
    10981 gcaacgcagc ggaggaacca agtttaaaat ctaccccttt gccaaagaca aattcttctt
    11041 tgacgaaagc actaccacgc tcgaattcaa ccgggcagga ggcaagatcg aatccgtgac
    11101 ggcccatgcg gtttccggca aggaaacctg gcgcaaaacc gataaaaagc cgacgacgcg
    11161 caccgaaatt caacttgacc aggcgacact acagcaatat gtgggcaagt atgaagtgca
    11221 gccctctttt gcattcacct tcacgctgga agacgggcaa ctgatgacgc aggctaccgg
    11281 gcagcccaaa tttccggtgt ttgcagagaa aaaagatttc ttcttcctca aagtggtaga
    11341 ggctcagatc gagtttttcc gcaacgacgc cggagttgta gaacgcctgg tattgtatca
    11401 gggcgggcaa aaaatcccgg ccctcaagaa gtaaggggcg ataaaaaaat aaagtgatca
    11461 ttttcgccaa aggattagat aagtaaacgt ttcattttaa gtgtcttatc taatcctttg
    11521 gcctaaagaa aatgagcatt tatttttgcc ccactactaa tcctttgtta ctaactgaca
    11581 atcagaacaa tcgcgtgaaa ttacagcacg cgattgttct tgtataagaa cacatacccc
    11641 tcatcagttg tattgtatcc aaaaaaacaa acaattgatg ttcagagctt tttcaatagc
    11701 cgcagcggcg ttcttcttgt ccatttttac ccattcgctc cacgcccagt cgcccgacag
    11761 cgcccgtgtt tttacgcagg aggagctgtt tgagtggctg ctcctgtacc accccgtagc
    11821 ccggcaggcc aacctgctcg acgatgaggc gcgcgctcaa ctgcgcatgg ctcgcggctt
    11881 tttcgatccc aaactctatt cggagtggga gcaaaaatcg tttgacggca gcgaatactt
    11941 cactttcggc aacagcggcc tcaaggtacc cacctggtac gggctggaac tcaaggcggc
    12001 attcaatacg gctaccggca ttcaggtgga ccccgaacac aagctacccg ccgccggcca
    12061 ggcggtgttg ggcatcaacg caacgttggg gcggggcatg atgatagacg aacgccgggc
    12121 cgccctgcaa caagcccgca tttttgagca aagcaacgaa gctcaacgac gacttgtcgt
    12181 caacgacctg ctgatggatg ccgtgaaggc ttattgggag tggacggcgg cctatcacca
    12241 gcgggaggcc atcctacagg cactggagct tgcccgcgtt cggcagcagg gcattgtgga
    12301 agcttaccgt cagggcgaca aacccgccat tgatacactc gaagcattca ttcaggtaca
    12361 aacccgtcag gccgacctca atgacgccaa tgtgagctac ctgaataccg gccaaatgct
    12421 ctccaatttt ctttggtggg aaaacgatac gcctctcgaa ctgacaggcc ggctgcggcc
    12481 cgcgccctta gacgaatcgg tagggagtgc agtactgcct tcagtagagg aactggcgca
    12541 acaagccgcc gaaaatcacc cgattctaaa attttatttt ttgaaaatca acactttgga
    12601 agtagatcgg cgattggcgg cagaacagct taaacctcag ttggatgtgg agtacaatct
    12661 attgggcaac gggctgaatc tggcgtacga tgccaacggt ggaggttcct ctttcaataa
    12721 cctctttttt caaaattata aatgggggct gcaattcaac atgcccattt tcctgcgaaa
    12781 agaacgggcc aaactcgaac tgacccgtct caaaattcag gacagcgggc tggagcttca
    12841 gcaaaaacga ctggaggtgg ccaacaaagt cagggcatac tttaatgaat ggcaaaacac
    12901 ccaacagcaa gtgtcgcttt accaaagcat gcaaaccaac tatcgcgcac tgctcgatgc
    12961 ggagatcacc cgcttccaat tgggcgaaag ctccatcttt ctcatcaatg cacgggagca
    13021 gaaattggtg gaagtacaac ttaaactact caaattgttg gccgaatttc aaaagcaacg
    13081 catgtacacc gagtgggccg tagggcgctt aggctgagca attcaattga tttccagacg
    13141 gtaggagtcg aaaatctgca tcctgattct tttggcagta tttttttgga ataaaaaata
    13201 ttaaaatatt ttgtttgaat aaattttttg ttttaccttg caggcgtttt cgaatcaaac
    13261 ctaaaaacat aagttatgat acgcgacgat cggcgccagt tgaatgagcg atttatcagg
    13321 gtgttccgcc tgctggaaga acggggagat attgtcaaaa atgaccgggg cggcaaaggc
    13381 atcggcgatt ttgctgaaaa aatattgggc aaccgggctt acggccacat tgttcgggca
    13441 tatctgaatg aagacgacaa gcgctgcatt gactatcaac aagcgcgcat catctgccgg
    13501 gaatacggca tcaacgaatc gtatctcatt gacggcatcg gcacgccatt cggattcgat
    13561 ctgcccgcgc cgcgttatca ggaacccacg ccttcagcaa ggggcaatat tctcttcact
    13621 acggtggaag cctttgccgg tacggccgtt gacgtaggcg gttttgctgc cgaagacctc
    13681 gaatttttca gcattccggg tttgtccggc ggtggcttgg tgtctttcgc catcaaaggc
    13741 aactctatgg agcctgttat ttccaacgga gacatcgtca tctgccggga ggtagccgga
    13801 gcgcacgaaa tcaaagacaa ccgcatttat gccatcaaaa acaacggcag tttgtgggta
    13861 aaatatgtgc agcgcctgcc cgacaacaga ggccgcaccg cccggctgaa actcatttcg
    13921 gccaaccacc tcgaacacga acctttcatc gaagaagtga atgaacacac ccgcgtatat
    13981 cacgtcattc gccgggtgag caatttgtaa tcataaccca acttactgat acttaaactt
    14041 ctacaagggg cggcatttcg cttggttgag tgtcgctccc cttttccttt cggctgccgg
    14101 tttgctgaaa gacatcatag gtttgcctga agaccgacct cgctaagatt taacctgctg
    14161 tttttgattt ttgcatcccc ggagcacgcg ctgccgggga ttttttttct atgctcgctt
    14221 aagtcttttt caccctgccc ggattgttgg caatgaatga accccaacca ctgcccgact
    14281 tggcgctgct tgcggcgctg ccgttgcggg tgctgtaata atgacaaacg gctgcggcca
    14341 gagcgtcggt ggcatccaga atctggtcct gcaaattgac ccccagagta gtggaaagca
    14401 tagcagacac ctgctctttg gaggcattgc cgttgccggt tacaacctgt tttattttct
    14461 tgggcgagta ctcagtgatg ggcactccct tggtgatggc cgccgccatg gccacgccct
    14521 gcgcccggcc caatttgagc atagactgca cattcttgcc gaaaaacggc gcctcaatgg
    14581 ccatttctcg gggcagataa tcggagatga ttttttgcaa ttgcgtaaaa atgcgctcta
    14641 agcgatacgg gtgctcttcg tcttttttga agtgaatgac gcccaagtgc aacaccgtca
    14701 gggttttgtt gtgtatttcc aaaatggcat aacccaacac gttggtgccg ggatctacgc
    14761 cgaggatgcg atgagtagtg gtttgcgagt tcaaatttgt agtattgcga aaaaaacgat
    14821 tgcaagataa gacaaaaata cgccggaaag acagaaaggc gcgccgggtc ggtctgattt
    14881 tcaaaacggg gtttgtaatc ttgtttgcag gcttgctgta tcggcatatc cgaaccatgc
    14941 cggtttcggg caattgggat gctctgttta catgggagcg attggaaaaa gagggcgttt
    15001 ggctactgct tgccgttgca ttgatgccgc ttaactggtc gctggaaacc ctcaagtggc
    15061 agcgattgat gtgcgttttt ggcgctgtgt ctttcaggcg cgcttttgct gcggtgcttt
    15121 cgggcgttac catctcactg tttacaccca atcggatagg cgagtacggc gggcgtttgc
    15181 tggcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctcgctgctg ggcacctgga
    15241 tacaatggat cgtattgctg gtcggcgggg tgttggggtt ctaccacctt gcagtccaat
    15301 atgagttgac agaaacgggc cgggcggccg ggctgggcgc agcgggtatg gctttggcgg
    15361 cgggcgctgt gctggcgctc tttcgcatcc ccgtgttggc ggcgctggcg caatggcatt
    15421 tgcgcggcca atggcgacgg cgagtattga gcaagctgtt gccgctgagg cattattcag
    15481 gcagcagcat cggcctgtct ttactctggg cggcactgcg atacatggtt tactgcacgc
    15541 aatactacct catgctgcgc ttttttggca ttcacatacc tgccggagag gcggctgccg
    15601 gcatagcggc tatatttttg gcacagacca gcctgccctt gcctccattg gccggcctga
    15661 tagcgcgggg cgaactggcg ctttgggtat gggggctgtc gggcgcagat acagcttcca
    15721 tattgggcgc cgcttatggc ctttttataa taaatttgag cctgccctcg ttagtggggg
    15781 cgtggatttt acttcgcctc aacctccgta aattttggaa aaatgaacgc aaaaacatgg
    15841 aaaacagcgc tttggatgcc gctgctgctg attctgaccg cctttgactt cccttatacg
    15901 acgggacaaa gcgcagacac ggccaacctc tgcgaaacga gcaactctgc cttcaaagcc
    15961 ggcgaagaac tcacttataa agtgtattac aactggaatt tcgtgtggct ctctgccggc
    16021 gaagttactt tccgggtgcg cgatgtgaat ggcgaatacc acctctcggc gcatggcagt
    16081 acttacaaat cgtacgactg gtttttcaaa gtgcgcgaca aatacgatac ttatgtggat
    16141 aaaaaaacgc tgctgccccg cctctctatt cgcgatgtga acgagggcaa atacagcctc
    16201 tacgatgccg tcacctttga ccgaccagcc ggtaaagcca cctctctgcg tggcaaaacc
    16261 aaagacatag ccacgcccac cgactatacc atcaacccct gcgcccacga cattctctct
    16321 atcctgtact acgcccgcaa cctgaattac agcaagatgc gcagcgggca gaccattccc
    16381 gtcaatattt tcattgacaa agaggaatgg aacctgaaag tctcgtatct gggcaaggag
    16441 gaaagcaaaa aagtgagaaa cctggggcgt ttccgggctg taaaactcag cccgcaggtc
    16501 atcgaagggt atgtttttaa aaaagacgcc cgcctgacaa tctgggcctc cgatgacgaa
    16561 aaccgcgtac ctttggtcat cgaatcgccg gtatcggtgg gttcggtgaa ggtgatcctc
    16621 aagagctaca aaaatctgcg ctacccggtc accagcaaag tgggcgaaga tgacggggtg
    16681 gatgaagacg ggatcaagga ttgacagtca acgaatcctt gaaaaacaac acattgcaaa
    16741 tgagtaaaaa tttaccaaat gaataaaaga gtggccgtcg gcatggtagc ggttatactt
    16801 atggttgccg gctttttcat tggccggagc ctgtatcgcc aaaaacagga aatcgtggcg
    16861 caggagcagt cgcaggtctt gttggaacgc atagaacatg tggccaagct catcaccgta
    16921 gagggctatt tttcagaagt ttatgattac aaagactact ggcgcttcga ctggtcgccg
    16981 ttccagaaaa aagcccttct gcgcgtgaag gctaaagtgt ccgtagggta tgatctgaca
    17041 aaaatgaaat tagaatcccg gcccgaagaa aagctgctca ttgtcagcca cttacccgac
    17101 cctgaaatac tctccactga ccacgatgta gattattacg acctctcgga gggcgttttc
    17161 aacagcttca cagaggcgga ttataccaaa atgaaccgca acgcgaaaaa ggtcattgaa
    17221 gagcaggcca gaaaaagcga cctgatgctc agtgccgaaa aacaagccta cgaaatatta
    17281 gagttgatgc gattcatggt agagagttcc ggctggcgca tggagtttca acccgaaggg
    17341 ctgaagcagg aagagaaaaa ggggtaggct atggtgctgg ttaaagaggg atgctcggga
    17401 aaattttgag gtaaataggg ttcgaaccta cgaccctccc gcgcctgtag cgcgggatgc
    17461 tctgaaccaa ttgagctatg caccagcgat caatcgctcc attcggcttc tcgacttcca
    17521 ggctttgatt tgacgctcga aggcggcagc ttcctttttt tcaggaaatt ccttggtaaa
    17581 aacaatgcgc caatccttgg tagtggggca aaaataaatg ttcattttct ttaggccaaa
    17641 catgagtcat cccgaatttt gtaaatgatc tcggagagat caaatatttg tagatcaaca
    17701 aattaaaatg cgtgacgacc tcggagaggt cgaacatgaa catgccaaaa taacacgttg
    17761 tgcgacctct ccgaggtcga aaaaaccgtt attgaggcat ttctacaaat ctgtgacctc
    17821 tccgaggtcg aaaattcggg atgactcatg tttggcgaaa atgaccactt tattttttta
    17881 tagccccttg gcttttgcgc taacttgtgg tcgctcaggt gtcgccggag cctctcctcc
    17941 atgctttgtt cggtgtgccc gatatagtac ttgtctaata tttctgagta aagactgtaa
    18001 gtaatggcca taaatggaaa agccactcct aaatggagtg gcttcgtggg cacagtaggg
    18061 ttcgaaccta cgaccccctg cttgtaaggc aggtgctctg aaccagctga gctatgcgcc
    18121 ctttgtttgc gctgtttggt gggcgatact gggctcgaac cagtgacctc acgcgtgtga
    18181 agcgtgcgct ctgaaccaac tgagctaatc acccttcgtc aacagcggac gcaaaggtaa
    18241 gcgcgttttc caaaacacac aatactcgac gtgaaaaaat ttaaaaattt cctgaaaaat
    18301 ggaaattcta agccgtcaaa cgccccctca cctctgctcc agcatcataa tatctttccc
    18361 gaaaacctcc acccggcgat aatgcgcctg aaggtgccgt gtgaataagt tgtctctcca
    18421 gcgcccttgg tacaagaccc agcgcggcga ctgcccgatg atgtgctccc attcccggcg
    18481 ctcgtcaatg cccaatgccg cgatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctgcatg cgctcgcgga gccagttgct
    18661 gacgatgcgc gggtagtcgg gcttgtcgaa gtagtccttt ttttgcaaca gcacattggc
    18721 caacatcagc agggccagca acggataacc caaccgccgc tgcaataaag gccgcaacca
    18781 ccggggcagc gcatcgctcc ggccggcagc aaaaaactcg ccggccagca gcgcaaatac
    18841 cggcatgagt tgtatgaaat agtgcccaaa caggcggccg gnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnttttaaa taatccggca aacttctgct caccgggtag tttttcatca cctcaaaggt
    19081 gtaaaaacga aaggtatcgc ctaatccggc ctgcgcatag tacgcccacg ccagcgcaaa
    19141 aggcagcaac atggccacca tcatcagcag ggcgcgcagc cagaaaaccg gccaccccga
    19201 cttgaactgc gcctgccgcc accataaaaa caagccc
//
LOCUS       DAORLF010000033        18352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1628_length_18352_cov_2.284855, whole genome shotgun sequence.
ACCESSION   DAORLF010000033 DAORLF010000000
VERSION     DAORLF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18352
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_18352_cov_2.284855"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK198_04825"
     CDS             <1..610
                     /locus_tag="PK198_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRF38089.1"
                     /translation="IKAAQRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNK
                     VGSLNKINKAFSELEQEYEREPSAEELAEMLEIPTEEVETTLGVAARHVSMDAPFVEG
                     EDNSLLDVLENIGTPGTDADLEYRESLSREIERSLSTLTDRQCDVIKLYFGIGIEHPM
                     SLEDIGDKFGLTRERVRQIKDKAINKLRATNRSKLLKHYLGA"
     gene            774..2315
                     /locus_tag="PK198_04830"
     CDS             774..2315
                     /locus_tag="PK198_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRF38090.1"
                     /translation="MRLRFHWAFLALLFLLSSCRVSREAALQSVDFTILQINDVYEIS
                     PLEGGKSGGLARVATVKKELLRENPNTIAVLAGDFLSPSFIGTLKTDSGEPIAGRHMV
                     ETLNAMGLDYATFGNHEFDLRDPDLLQQRLHQSAFQYTCCNVLRVKDGREMPFTHMVN
                     GTAQPIPPYILREFTGRNGSKARIALVGVVLPFNKQPYVKYLPDAETFRSTCQSLEGK
                     AEVILGLTHLAIDADMALAETTPQVPLFMGGHDHNHMGHFVERTVIAKADANAKTVYI
                     HRLSYYPATKTTQVRSTLKVIDDKIPADPATQAVVEKWESQVFGLAEKMGYQPRRVVM
                     NTAEPLECTETIIRSSQTNFGQLAVEAYQAAMPGADVYWINSGSMRLDDRLSGAITEF
                     DVMRTFPYGGKIVKMQLPGAVVQEALRISMTTNKGEGGYFQLLHADPKPDAQGRFLIN
                     GNPIEPERMYSVVMPLFVAEGKEANLSMLGKYKFDVLENLSPAPGASIRNDVRDIVIS
                     YMSRK"
     gene            2324..>2924
                     /locus_tag="PK198_04835"
     CDS             2324..>2924
                     /locus_tag="PK198_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF38091.1"
                     /translation="MNYEKVLLSPGGPAVSPIVTGVMKWGLWGRQMGTTQMLHLIEAS
                     VEAGATTFDHADIYGHYTTEAEFGAALKNRPELRSRIQLVTKCGICLPGPHRQYGLKH
                     YDTSAAHIRRSTEQSLRNLHTDYIDLLLIHRPDPLMHPQEVAETFEALRSEGKVLHFG
                     VSNFSPAQLETLAAFTPVVTNQVQANAMHLAPFSDGTFDQ"
     assembly_gap    2925..2953
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2954..>3179
                     /locus_tag="PK198_04840"
     CDS             <2954..>3179
                     /locus_tag="PK198_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF38092.1"
                     /translation="PMAWAPLGGERFFTHPDDPQVSRVRTACETIAASREGIHSDQVM
                     LAFLLRHPAGIVPVIGTGNAERIQSSMKALD"
     assembly_gap    3180..3278
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3349..3747)
                     /locus_tag="PK198_04845"
     CDS             complement(3349..3747)
                     /locus_tag="PK198_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRF38093.1"
                     /translation="MKIASILSFILALTLSACLTDNTAQLLQGDWRVISIEATGQAVE
                     NAPADYILQFGEDGNLSLRLDVNNCGGRFNTFNGGNIDIEPMGCTEACCDTPFAQQLA
                     GLLSEMEHFDIQGRTLTLHGAAGLVVARAE"
     gene            complement(3744..4613)
                     /locus_tag="PK198_04850"
     CDS             complement(3744..4613)
                     /locus_tag="PK198_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38094.1"
                     /translation="MRTLLILIISCCIVGLATAQCPFTPTVTGDLLVCPEARTTLGTQ
                     AYDAYQWYTRPFGSSAAPQPVNGATGPAFTVDYNDTPLYVSVSATLNNCTERSAEVLV
                     DGLVFLLPVALSDGEFTIGPNGEAVICAGDTMHLIALSPYTTNIKWFDGEEAIPGAND
                     DTLIVTRPGSYWFTGAPQQCPDFTASLGLRVEVVWSDIPGCATAVRELQIQDVLVWPN
                     PASDRVAISVAHPGAINLTLLDITGRPLWRSYFDGQTEVPMSAWPEGMYILQLSAADG
                     AMSRYKLIKNSKQ"
     gene            complement(4752..5354)
                     /locus_tag="PK198_04855"
     CDS             complement(4752..5354)
                     /locus_tag="PK198_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF38095.1"
                     /translation="MKSILLSTILAVLAAAALIYTGIDWRWAKLSENVILLQYIGIPS
                     VFIGILVPGAMPLYLYLKRKQQPHYSTMSAAAFKAFLAAWGYSTLLKAFTNRVPREPF
                     EALGMTDLSGQFRLGFLQGNNLWESLIEGFPSGHTMTAFAMSVAVLPFMASGTKRTLA
                     ILYAVYIGLGVSLTVHWLSDAVAGGLIGYALGKMIAATKT"
     gene            complement(5351..5986)
                     /locus_tag="PK198_04860"
     CDS             complement(5351..5986)
                     /locus_tag="PK198_04860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF38096.1"
                     /translation="MDTRMQSTLARLHQAANREILTIAKGLAKGIFRPLQPEDMKDAY
                     IAMSREQGEVMVRLIQENHCTNIVEFGTSFGISTLYLAAGAKETGGKVITTELLPEKC
                     AVAQKNFEEAGAADIIDLREGDAMQTLRDCPADIDFLVLDGWNDLYLPLLKMLEPKFK
                     KGCIVITDNTNFPSAKPFLNYIRNHPNYTSEQLPTDKGGTEKSVWLGNRTL"
     gene            complement(5992..>6186)
                     /locus_tag="PK198_04865"
     CDS             complement(5992..>6186)
                     /locus_tag="PK198_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38097.1"
                     /translation="VTQDIRGKEFPLFGVKAGETVGDYDLIYIFNDMLFMGSKHVDGR
                     GFDKPENRPTNLQVPIYRKW"
     assembly_gap    6187..6219
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6220..>6560)
                     /locus_tag="PK198_04870"
     CDS             complement(<6220..>6560)
                     /locus_tag="PK198_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38098.1"
                     /translation="KTYVQGGEWVSISPELRPFEDRLGAGKIEPFYVQRNFQYFPNDR
                     FEGSILSSADPYGQVPLVKFTFKGHTIWGQAHPIAEGAYEVDYILDEGFEVTPLHLMF
                     ADQLNTAPVAE"
     assembly_gap    6561..6574
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6760..7092
                     /locus_tag="PK198_04875"
     CDS             6760..7092
                     /locus_tag="PK198_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF38099.1"
                     /translation="MRKNKNFNPNNCPVTHCINLIGGKWKPVVIHLVRKDCNRFSLLQ
                     RAIPDISKQMLTQQLRELEEDGLIERKIYAEIPPKVEYSISAYGKTLFPVIDAMSVWG
                     LNDLNISG"
     gene            complement(7115..8047)
                     /gene="xerD"
                     /locus_tag="PK198_04880"
     CDS             complement(7115..8047)
                     /gene="xerD"
                     /locus_tag="PK198_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRF38100.1"
                     /translation="MNWTTALKGFKAWLMLERALSQHTIEAYLGDVGKLEQYIQLSGL
                     ELQPAQVSTDHLSAFLMWINELGLGARSQARLLSALKTFYKYLLTENTITTDPTELLD
                     GPRLPRHIPEVLSFDEIQRMLSVIDLSEPHGVRNRAMLETLYACGLRVSELTELRLSN
                     LYLDIGFVKVIGKGDKERIVPIGEEAVKHIRLYMEGVRQTMLNIHKDHANYLFLNRRG
                     KKLTRVMVFLIIKEIAGLAGIEKNVSPHTFRHSFATHLIEGGADLKAVQDMLGHESIL
                     TTEIYTHLDTDYLRETVLQYHPRNRGGDSRATDE"
     assembly_gap    8145..8154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8155..8712)
                     /locus_tag="PK198_04885"
     CDS             complement(<8155..8712)
                     /locus_tag="PK198_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRF38101.1"
                     /translation="MSSFLPYLPPRYDATEMLQRSRDFYQEMNTRRTLRMFSDKPVPR
                     ELIEQLLMTAGTAPSGAHKQPWTFCAVGDPQIKAQIREAAEKEEYVNYHGRMSEEWLQ
                     DLEPFGTDWNKPFLETAPWLLVVFKKSYDLEGEEKKKNYYVNESVGIACGFLLAAIHH
                     AGLAALTHTPSPMNFLQQILGRPDNE"
     gene            complement(8775..9248)
                     /locus_tag="PK198_04890"
     CDS             complement(8775..9248)
                     /locus_tag="PK198_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRF38102.1"
                     /translation="MTTRTTDDIIKEVKNIFSSHLENGGFRKTPERYAILEEIYRRSD
                     HFDAEALYIHMKNQNYRVSRATVYNTLDLLVSCDLVKKHQFGKNLAQFEKSYGFKQHD
                     HLICVDCGRVLEFCDPRIQQIKNMMGDLLHFNVTHHSLNLYGQCNGACPRTGGKE"
     gene            complement(9306..9686)
                     /locus_tag="PK198_04895"
     CDS             complement(9306..9686)
                     /locus_tag="PK198_04895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRF38103.1"
                     /translation="MVAERAGYCCEYCRLPEPFAHYRFHIEHIIPIQHGGKTDLDNLA
                     YSCSFCNWKKGPNLGTLLSSESPIIRLFHPRLDVWTDHFSMEDGVILGKTEVGEATVR
                     LLEFNHPDNIIERRLLIELGRYPL"
     gene            complement(9717..9962)
                     /locus_tag="PK198_04900"
     CDS             complement(9717..9962)
                     /locus_tag="PK198_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38104.1"
                     /translation="MVTPEVNVFDELAAFLAAMNPEKVLGFHASPQAQQRMEELQWKH
                     KELGLSALENEEMERYLTVERIVRLAKIHAAQRLVVQ"
     gene            complement(9988..>11880)
                     /locus_tag="PK198_04905"
     CDS             complement(9988..>11880)
                     /locus_tag="PK198_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF38105.1"
                     /translation="QIAKMVDGLTKLDSAYNAESQQAENVLKVLSTLVEDVRVVLIKM
                     ADRLHNMRTLGAMTRQKQLKIAAETSYVYAPLAHRLGLYGLKTEFLDLCMKVTEPEAY
                     HDIARNLADTKRDRNRYINEFIKPLTPHLDAIGVPYQVYGRPKSIYSIWNKIQSKRVP
                     FEEIYDLFAVRIVMDVPLDRERLSCWQAYSIVTNVHKPLPERLKDWVTMPKSNGYESL
                     HTTVIGPQGKFVEVQIRTNRMDEIAERGFAAHWKYKGVNNQKDVFEQWLDSVREILED
                     PGENAVEVLETFKDNNLFNEEIFVYTPKGELRILPKGATALDFAFSIHSDVGYHCTAV
                     KVNNKLVPMGCVLKNGDQVKVITNKNQKPSEDWLKFVVTGKAKSKIRSAMKEERRKLG
                     EYGREAFERKLRNLKLEPNEEIVEKLVRYFDLDSHVDFYYSIYMGAITIERIFKLFMP
                     EGNTLVEIPKVGEEVRPPEPPPSKKRDLSKGRLMINGEDAERYSYSLATCCNPVQGDD
                     IFAYVTSNATLKIHRTTCPNATNLMANYGYRIMKAEWVHTPGTTFVADLKITGIDDGP
                     GVIERLSHAISSNLNINIRTFSIAGDQGYFEGRVSLVVQNTDQLSIAIRSLLKLPNVS
                     TVERVV"
     assembly_gap    11881..11894
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11895..12205)
                     /locus_tag="PK198_04910"
     CDS             complement(<11895..12205)
                     /locus_tag="PK198_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF38106.1"
                     /translation="MTAISNMSPEDKMVHNAYRKLLRTIKTPLEDADRKLIRDAYELA
                     VEAHRPQRRKSGEPYILHPLAVATICTEEIGLGPTAVAAALLHDVVEDTPVKLEDIRA
                     K"
     gene            12337..12418
                     /locus_tag="PK198_04915"
     tRNA            12337..12418
                     /locus_tag="PK198_04915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12371..12373,aa:Leu,seq:tag)
     gene            12597..13619
                     /locus_tag="PK198_04920"
     CDS             12597..13619
                     /locus_tag="PK198_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRF38107.1"
                     /translation="MPTFQREAISDLHFKLTATLAPSDYETEFHTQLEKYRKEAAIKG
                     FRKGKTPASVVRKMFGKSVLADILNKKLQEAIPAYVREQHLDMLGGPIPSDENDAIAL
                     DAKELKEYSFIFEIGVAPEFDIQGTGPDAEYERLDVIASEERINEHLEGLRNEDLRAV
                     AVTENVSENGEARLRFTELDGDAPRENGIVTSCSVQVRDIKDDALRNQILAAKIGDTF
                     QIADIFEDLNENSALVRNQLLQSHDDNGIQINPGFEAVVEELNSREPAPFDEEFFQYA
                     FGEDTDVTTEAEAKARIKKDIEEYLAGSSQSLLRAQVRDRIMELNQFPLPEAFLKKWL
                     LYSNEE"
     gene            13616..14053
                     /locus_tag="PK198_04925"
     CDS             13616..14053
                     /locus_tag="PK198_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38108.1"
                     /translation="MTEEDVESQFDSIAKSFRWSTISQRLAKRFEIKVSEEEVQQYFI
                     NQVRQMLGGYAGLGDSFMLSMAQRLMEREESVMEAYEDIYNTKMLAAVAGEVKQVGKP
                     VTEEELQQAVQAYNAKHKPQQPESDDIVDAEEVQPEPEAEAGE"
     gene            14139..14843
                     /locus_tag="PK198_04930"
     CDS             14139..14843
                     /locus_tag="PK198_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRF38109.1"
                     /translation="MFRKDEFFKYAVKHLGMSSYKVEDYARYNVQSMSQVQNMTPYII
                     EERQLNATVLDVFSRLMMDRIIFMGTPVYDDIANIVQAQLLFLQSADPKRDINMYINS
                     PGGSVIAGLGIYDTMQFVSPDVGTICTGLAASMGAVLLAAGAKGKRTCLYHSRVMIHQ
                     PSGGMQGQVSEMEISYKLIKQMQEDLYKILSHHTGKTYQQIEEDSDRDKWLTSQEAMN
                     YGLVDEVLGKGVEAAG"
     gene            14955..16214
                     /gene="clpX"
                     /locus_tag="PK198_04935"
     CDS             14955..16214
                     /gene="clpX"
                     /locus_tag="PK198_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRF38110.1"
                     /translation="MRKSRQNYHCSFCGKDKSEALLLIAGIEGHICEACVEQAGQIVQ
                     EELYGNKQEGGKKEKSKSGAGFSVVKPQEIKAYLDQYIIGQDDAKKVLSVAVYNHYKR
                     LGQPRKDDVDIEKSNILFVGQTGTGKTLLAKTIAKFLNVPFAIVDATVFTEAGYVGED
                     VESILSRLLQVCNYDVAAAERGIVYLDEIDKIARKSDNPSITRDVSGEGVQQALLKML
                     EGTDVNVPPQGGRKHPEQKLVKVNTENILFICGGAFEGIDKIIARRMNNQVIGFKADD
                     YVRVDSDNMLQYINHQDVKHYGLIPELVGRLPVLAYLNPLDQETLRRILLEPKNSLIR
                     QYERLFEIEGIQLSFEPDAIEFIAEKALEFAFGARGLRSICEAIMTDAMFELPSQNEV
                     RHLHITRAYAAEKLSRSNLGKLRAKAA"
     gene            16270..17706
                     /locus_tag="PK198_04940"
     CDS             16270..17706
                     /locus_tag="PK198_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38111.1"
                     /translation="MNRIIALLLFLSVGMVQSIVGQQIIMSEDIPLRNDVSYELLGEF
                     KGRVLLFRDQISKYEVQGFDQRLRPSWSKILTLDKKNPKVIGTTHSRNDFTLIYHYRD
                     QGNTILKAAKFDPGANMIDSATIKNLGSVFFTPDFEITYSEDRSKILVYYIERQAELH
                     AYVFDAVGFKLLWEKTIVPSGFSFFEDFLQVVLDNDGNMCLIFEKDKFVARRDGHHYH
                     IIGVIGAEDRLVQFQAPLEGKLTYDVLFTYDNLNDRLVAAGLYSDKAGDRATGYFILK
                     IDPLQRESPFLRFESFSDLFVSNLIGKEVEKNKGVTELSVREIVLRRDGGVLMIVERI
                     KEYERRLGAPNRVSFDGVGRFIVDYYYEDIVALSIHPDGQAHWETILPKKQYSQDDGG
                     VYSSYFLFKTPENLRFLFNDEIRFENTVSEYRLNGNGAFERRSLLNTEKLEVRLRFRD
                     AIQVSADAFVVPSERRNRLRLVKVIFEE"
     gene            17811..18170
                     /gene="rplS"
                     /locus_tag="PK198_04945"
     CDS             17811..18170
                     /gene="rplS"
                     /locus_tag="PK198_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRF38112.1"
                     /translation="MDAIKFVHEQMSPERNFPEFFPGDNITINYKIIEGDKERVQAFR
                     GDVIQISGHNGTRTFTVRKMSNGVGVERIFPFSAPNIVDIQVLKRGKVRRARLFYLRD
                     LVGKKARIKERKFVAKK"
BASE COUNT         4372 a         4635 c         4741 g         4405 t
ORIGIN      
        1 tatcaaagca gctcagcgct tcgatgaaac acgtggattc aaatttatct cctacgccgt
       61 ttggtggatt cgtcaatcca tccttcaggc tttggctgag caatcacgta ttgtgcgcct
      121 gcctttgaac aaagttggct ccctcaataa aatcaacaag gcattttccg aactcgaaca
      181 ggaatacgaa cgcgaaccca gcgccgaaga actggccgaa atgctcgaaa ttcctaccga
      241 agaagtggaa actacgctcg gtgttgccgc ccgccacgtc tcgatggatg ctcccttcgt
      301 ggaaggagaa gacaactcgc ttcttgacgt gctcgaaaac atcggcacgc cgggaaccga
      361 cgccgacctg gaataccgcg aatcgctgag ccgcgaaatt gaacgctcgc tctctacgct
      421 caccgaccgc cagtgcgacg tcatcaaact gtacttcggc atcggcatcg aacatcccat
      481 gtctttggaa gacatcggcg ataagttcgg cctcacccgc gagcgtgtgc gccagatcaa
      541 ggacaaagcc atcaacaaac tgcgcgccac caaccgcagc aaactcctca agcattacct
      601 cggcgcctga ggaatcttac tttttttaaa aaaatgatcg tatagtcagg tagttcatac
      661 agcttcgcgc tgcatgaact gcctcgctgt gtgttccctt tccatacaat acgcgccgaa
      721 aataagcctc tgttttttac ctttgtggga actgtcacaa aaaagttcct cttatgcgct
      781 tacgtttcca ttgggctttc cttgccctgc ttttcctgct ttcttcctgc cgggtttccc
      841 gcgaagccgc tctccaatct gttgatttta ccattctgca aatcaacgat gtgtatgaaa
      901 tttcgccact cgaaggaggc aaatcgggcg ggctggcgcg cgtggctacc gtaaaaaaag
      961 agctgctgcg cgaaaatccc aataccattg ccgtactggc cggagatttt ctcagccctt
     1021 ctttcatcgg caccctcaag accgacagcg gcgagccgat tgccggacgg cacatggtgg
     1081 aaaccctcaa cgcgatgggc ctggattatg ccactttcgg caatcacgaa ttcgacctcc
     1141 gcgaccccga ccttctgcaa caacgcctgc atcagagcgc ttttcaatac acttgctgca
     1201 atgtgctgcg ggtgaaggat ggccgggaaa tgcctttcac gcacatggta aatggcacgg
     1261 cgcaaccgat tccaccctac attttacgcg aatttaccgg acgcaacggc agcaaggccc
     1321 gcatagcgct ggtgggcgtg gtgttgccct tcaataagca gccttatgtg aaatacttgc
     1381 ccgatgccga gactttccgc agcacctgcc aatcgctgga gggcaaggcg gaagtcattc
     1441 tcggcctcac ccacttagcc atagatgccg atatggcgct ggccgaaacc acgccgcagg
     1501 taccgctgtt catgggcggc cacgaccaca accacatggg gcattttgtg gagcgaacgg
     1561 tcattgccaa agccgacgcc aatgccaaaa ccgtttacat acatcgtctg agctactacc
     1621 cggccaccaa aaccacacag gtgcgctcca ccctcaaggt gattgatgat aaaattcccg
     1681 ccgatcctgc cacccaggca gtggtggaga aatgggaaag ccaggtattt ggcttagccg
     1741 aaaagatggg ctaccaaccc cgccgtgtgg tgatgaatac tgccgagcct ttggaatgta
     1801 ccgagaccat catccgcagt tcgcaaacca actttggtca attagccgta gaggcgtatc
     1861 aggcggccat gcccggcgca gatgtgtatt ggatcaacag cggttctatg cgtttggacg
     1921 accggctgag cggcgccatc accgagtttg atgtgatgcg caccttccct tacggtggaa
     1981 aaattgtaaa aatgcagctg cccggcgcag tggtacagga agcgctccgc atcagcatga
     2041 cgaccaacaa aggcgagggc ggctattttc aactccttca cgccgatccg aaacccgatg
     2101 cacaaggtcg ctttttgatc aacggcaacc cgatagaacc ggagcggatg tactcggtag
     2161 tcatgccgct gtttgtagct gagggcaagg aggccaacct gtctatgttg ggaaaataca
     2221 aattcgacgt gttggaaaat ttgtcgcccg ctcccggcgc ctccattcgc aacgacgtgc
     2281 gcgacattgt catctcgtac atgagccgca aataaacgcc gacatgaact acgaaaaagt
     2341 actgctctcg cccggcgggc cggctgtgtc gcccatcgtg acgggcgtca tgaaatgggg
     2401 cctctggggg cggcaaatgg gcacgacgca aatgcttcat ttaatagaag catccgtaga
     2461 ggcgggcgct accaccttcg atcatgccga tatttacgga cattacacta cggaagccga
     2521 atttggggca gccctgaaaa accgacccga attgcgcagc cgcatacaac tcgtcaccaa
     2581 gtgcggcatc tgcctgcccg gtccgcaccg gcaatatggg ctaaaacatt acgataccag
     2641 cgctgcgcac attcgccgtt ccacagagca atcgctccgc aacctccaca ccgattatat
     2701 tgatctcctg ctcattcacc ggcccgaccc gctgatgcac ccgcaggagg tggccgaaac
     2761 atttgaggcg ctgcgcagcg agggcaaagt actgcatttc ggcgtgtcta atttcagtcc
     2821 ggcgcagttg gaaaccctgg cggctttcac gcccgttgtc accaatcagg tccaagccaa
     2881 cgcaatgcac ctcgcgccct tcagcgacgg cactttcgac cagtnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnncccgatg gcctgggcgc cgctgggcgg ggagcgcttc ttcacgcatc
     3001 ccgatgaccc gcaggtgagc cgggtgcgca ccgcctgcga aaccattgcc gcaagtcggg
     3061 aaggcataca cagcgatcag gtgatgctgg cttttttgct gagacatcca gcgggcatcg
     3121 tgcccgtcat cggcacgggc aatgcggagc ggattcagtc ctccatgaaa gccttggacn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atcatacaga ttttatggcg
     3301 ccgacagggt gatctgttgc ggcatgaaaa aatggtgaag aaatcccctt actccgcccg
     3361 cgccaccacc aagcccgccg cgccgtgcaa agtcagtgtc cggccctgaa tatcaaaatg
     3421 ctccatttcc gacagcaagc cggccaattg ctgggcgaaa ggggtgtcgc agcaagcctc
     3481 ggtacagccc attggttcaa tatcaatatt gccgccattg aacgtattga accggccgcc
     3541 gcagttgttg acatccaaac ggaggctcag gttgccgtcc tctccgaatt ggagaatgta
     3601 atctgccggt gcgttttcta ctgcctgccc tgtggcttct atggaaatga cgcgccaatc
     3661 gccctggagc aactgcgccg tgttgtcggt gaggcaggcg ctcagcgtga gtgccaaaat
     3721 gaatgagaga atgcttgcga ttttcattgc ttgctgtttt ttatcaattt ataacgactc
     3781 atggccccat ccgctgcgct gagttgcaag atgtacatgc cttccggcca agcggacatg
     3841 ggcacttctg tctgtccatc gaaataagaa cgccaaagcg gtcgcccggt gatgtccaac
     3901 agggtcaaat tgattgcgcc gggatgcgca accgatatgg ctaccctgtc agatgcaggg
     3961 ttgggccaaa caagaacatc ctgtatctgt agttccctca cggcggtggc acagcctgga
     4021 atatcgctcc agaccacctc cactctgagg ccgagggagg ctgtgaaatc cgggcattgt
     4081 tggggcgcgc ctgtgaacca atacgatccg gggcgggtca cgatgagcgt atcgtcgttg
     4141 gcgccgggaa tggcctcttc gccgtcaaac catttgatgt tggtagtgta aggcgacagc
     4201 gcaatgagat gcatggtgtc tccggcacaa atcacggcct ctccgttggg gccgatggta
     4261 aactcaccgt cagacagcgc caccggaagc aaaaacacca gcccgtccac cagtacttcg
     4321 gcgctgcgct cggtgcaatt attgagcgta gctgatacag agacatagag cggcgtatcg
     4381 ttgtaatcaa cagtaaaagc agggccggta gcgccattca ccggctgcgg cgcggcgctg
     4441 ctgccaaagg ggcgcgtgta ccattgataa gcatcgtaag cctgggtgcc gagcgtggta
     4501 cgggcttcgg ggcaaacgag caagtcgcct gttacggtgg gggtgaaagg gcattgggca
     4561 gtggcaagtc ccacaatgca acaggagatg atcagaatta gtaaggttcg catgttatat
     4621 ggtttttccg tccgttggaa tagacgtgaa aaacccttaa ttggttagtc gaatctttac
     4681 ggtagaagat agaaatcacg ttgcccaagt cattttgagt caaactgttg gcagcaatat
     4741 cagacaaagc cctatgtttt tgttgccgca atcattttgc caagagcata tcctatcaaa
     4801 ccacctgcta ccgcatcaga caaccaatgc acagtgagtg aaactcccag cccgatgtac
     4861 acagcgtaga ggatagccag cgttcttttt gtacccgatg ccataaacgg taataccgcc
     4921 accgacatgg caaaggccgt catggtatgc ccggagggaa aaccctctat caggctctcc
     4981 cacagattat tgccttgcaa gaaacccaac cggaactgcc cgctgagatc cgtcatgccg
     5041 agcgcttcaa aaggctctct gggtacgcgg ttggtaaagg ctttcagtag ggtggaatat
     5101 ccccaagccg ccaaaaatgc cttgaacgcc gctgccgaca tggtgctgta atgcggttgt
     5161 tgttttcgtt tcaggtacag gtacagcggc atagcgccgg ggacaagtat gcctatgaat
     5221 acgctgggga tgcctatgta ttgaagcagt atgacgtttt cgctaagttt ggcccacctc
     5281 caatctatgc cggtgtaaat gagtgcagcg gcagctaaga ccgccaaaat ggttgacaag
     5341 aggatgcttt tcatagcgta cggttgccca accaaacact tttttccgta ccccccttat
     5401 cggtgggcag ttgttcagaa gtataattag gatgattgcg gatgtaattc aagaagggct
     5461 tggcgcttgg aaaattggtg ttatctgtaa tgacgatgca gccttttttg aattttggtt
     5521 caagcatttt taataacggc aaatacaaat cattccagcc atccaacacc aaaaaatcaa
     5581 tgtcggcggg acagtcccgg agtgtctgca tggcgtcgcc ttcccgcaaa tcaatgatat
     5641 cggctgcgcc tgcttcttca aaattctttt gcgcgacggc acatttttca ggcaagagtt
     5701 cagtagtaat gactttgcca cctgtttctt ttgctcctgc cgccaggtaa agggtcgaaa
     5761 tgccgaagga tgttccaaac tctacaatat ttgtgcaatg gttttcctga atgaggcgga
     5821 ccatcacttc accttgctca cggctcatgg caatgtaggc gtctttcatg tcttcgggct
     5881 gcaaagggcg gaaaatgccc tttgccaaac cctttgcgat ggtcaatatc tcacgattgg
     5941 ctgcctgatg caagcgagcc aaagtacttt gcatacgagt atccattgcg cttaccattt
     6001 tctataaatt ggtacttgca aatttgtcgg gcgattttcg ggcttgtcaa atccacgtcc
     6061 gtctacatgt ttactgccca tgaaaagcat gtcgttgaaa atgtaaatca agtcgtagtc
     6121 accaacagtt tcgcctgctt tcactccaaa aagcggaaac tccttgcccc tgatgtcttg
     6181 cgtcacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttcggctacc ggggcagtgt
     6241 tgagttggtc ggcgaacatg aggtgcaggg gcgtaacctc aaagccctcg tccagaatgt
     6301 agtccacctc gtaggcaccc tccgcaatcg ggtgtgcctg gccccagatc gtatgccctt
     6361 tgaaagtaaa tttcaccaaa ggcacctgcc cataggggtc ggccgaactt aggatactgc
     6421 cctcaaagcg gtcgttcggg aaatactgaa aattgcgctg cacataaaac ggctcaattt
     6481 tgcccgcgcc caacctgtct tcaaacgggc gcaattcggg gctgatgctg acccactcac
     6541 cgccttgaac ataagttttg nnnnnnnnnn nnnnctattg tcatttctga atgctttttt
     6601 tatgaaaaat tgaattgaca aggcaaagtt ctatatattt gtctttaatt caatagtagt
     6661 ctttgttttt gtttatagta aataaaaata gactattatg aaaatcaatg cattagagcg
     6721 gattgttttc gacttttcaa ccttattccc gttgtgccca tgcgtaagaa caaaaatttc
     6781 aatccaaaca attgcccggt cacccactgt attaacctga taggcggcaa gtggaaaccc
     6841 gttgtgattc atttggtcag aaaggattgc aaccgttttt ccttgttgca aagggccatt
     6901 cccgacatca gtaaacaaat gctgacccag cagttgcgtg aactggaaga agacggtttg
     6961 atagaaagga aaatttacgc agaaataccg ccgaaggtag agtactccat ttcggcgtat
     7021 ggaaagactt tgtttccggt gattgatgca atgagcgtgt gggggttgaa tgatttgaac
     7081 atatccggct aatcacagtg agggaaagga cgattcactc gtcggtcgct ctactatccc
     7141 cgccccgatt cctcggatga tattgcagca cggtttcccg caggtaatct gtatccaggt
     7201 gagtgtaaat ttccgtggtg aggatagact cgtggcccag catgtcctgc accgctttca
     7261 ggtcggcgcc gccctcgatg aggtgtgtgg cgaaagaatg ccggaacgtg tgcggactca
     7321 cgtttttctc tatacccgcc agccctgcta tttctttgat gatgagaaac accatgacgc
     7381 gggtgagttt tttgccccgg cgattgagaa acaggtagtt ggcgtggtct ttatgtatgt
     7441 tgagcatggt ttggcgcaca ccctccatat acaggcggat gtgcttgacg gcctcctccc
     7501 cgatgggtac aatgcgctct ttgtcgccct tgccgatgac ttttacaaaa ccgatgtcca
     7561 aatacaggtt ggaaaggcgc agttccgtca attccgacac acggaggccg caagcgtaca
     7621 gggtttcgag catggcgcgg ttgcgcacgc cgtggggttc gctcaggtca atgaccgaca
     7681 acatgcgctg aatttcatca aaactgagca cttcggggat gtgccggggc aggcgcggac
     7741 cgtccagtag ttcggtgggg tcggtggtga tggtgttttc ggtgagcagg tatttgtaaa
     7801 aagttttgag ggccgacagc agccttgcct gcgagcgggc gcccagcccc aattcattga
     7861 tccacatcag aaaagcgctg agatgatcgg tggagacctg cgccggctgg agttccaaac
     7921 ccgacaattg gatgtactgc tccaacttgc ccacgtcgcc gaggtacgcc tcaatggtgt
     7981 gttgcgagag ggcgcgttcc agcatcagcc aggctttgaa gcccttgagt gcggtggtcc
     8041 agttcatatt ccatcaagcc agatgatgaa ttcctctgcc gttttgcgcc ggagatcggg
     8101 tacaacagcg tcatcggcgg ggtagaccac cgggaagagc aggannnnnn nnnnctcgtt
     8161 gtcggggcgg cccagtattt gttgcaaaaa attcatagga ctgggcgtgt gcgtgagggc
     8221 cgccagaccg gcatggtgaa tggccgccag taaaaagccg caggcaatgc ccaccgattc
     8281 gttgacgtag tagtttttct ttttttcctc gccttccaga tcgtacgatt ttttaaaaac
     8341 caccaacagc cagggcgccg tttcgaggaa gggcttgttc cagtcggtgc cgaagggttc
     8401 caagtcttgc agccactcct ccgacatgcg gccgtgataa tttacatatt cctctttttc
     8461 agcggcttcc cgaatctgcg ctttgatttg gggatcaccc accgcgcaga aagtccaggg
     8521 ctgtttgtgt gctccggagg gcgctgtgcc ggctgtcatc agcagttgct caatcagttc
     8581 gcgcggcacg ggcttgtcgg aaaacatgcg cagggtgcgc cgcgtgttca tctcctgata
     8641 aaaatcgcgg cttcgttgca acatttccgt tgcgtcgtag cggggcggca gatagggaag
     8701 gaatgacgac atagtaagct tgctcaattt tacacggtac aaatccgggg gatcatctgc
     8761 aatggagcac tcaattactc cttgccgccc gtgcgcgggc aggcgccgtt gcattggccg
     8821 tagaggttga gtgagtggtg ggtgacgttg aaatgcagca ggtcgcccat catgtttttg
     8881 atctgttgaa tgcgggggtc gcaaaactcg agaacgcgcc cgcagtccac gcatatcaag
     8941 tggtcgtgtt gtttgaagcc ataggacttt tcaaactgtg ccagattttt gccgaactgg
     9001 tgttttttta ccaaatcgca gctcaccaac aggtctaaag tgttatatac ggtggcccgg
     9061 ctcacgcggt agttttggtt tttcatgtgg atgtacagcg cttcggcatc gaagtggtcg
     9121 ctgcggcggt atatttcctc cagaatggca taacgttcgg gggttttgcg aaagcccccg
     9181 ttttcgagat gcgaggagaa gatgtttttt acctctttga tgatgtcgtc ggtagttctt
     9241 gttgtcatac ttataataac tacaccacgc gctctacggt tgagacgttg gggagtttga
     9301 ggaggctata gtgggtatct gcccagttcg atgagtaatc ttcgctcaat gatgttgtcg
     9361 ggatgattga actccaacaa ccggacagtg gcttctccta cttcagtctt ccccaaaata
     9421 accccatctt ccatactaaa gtgatctgtc catacatcca gccgtggatg aaaaagtcgg
     9481 atgattggac tttctgaaga gagtaatgtg ccgaggttgg gacctttttt ccagttacaa
     9541 aaagaacaag agtatgcgag attatctaag tctgttttgc ctccatgttg aatgggaatg
     9601 atatgctcaa tatggaacct gtagtgcgca aacggctcgg gtagcctgca atactcgcaa
     9661 caatagcctg cccgttcggc aacaatgctt cggaaagctc cggatacctt aatagctcat
     9721 tgaacaacaa gcctttgcgc tgcatgtatt tttgcgagtc ggactattcg ttcaaccgta
     9781 agatagcgct ccatctcttc gttttccaag gcggagaggc caagttcttt gtgtttccat
     9841 tgtagttcct ccattcgctg ctgagcttgt ggcgaggcat gaaaacccag taccttttca
     9901 ggattcatag cggctagaaa ggcggccagt tcatcgaata cgtttacttc gggcgttacc
     9961 ataagcattt gtttacataa gatataacta caccacgcgc tctacggttg agacgttggg
    10021 gagtttgagg aggctgcgga tggcgatgct gagttgatct gtattttgaa ccaccaaact
    10081 cacgcgcccc tcgaagtacc cttggtcgcc ggcgatgctg aatgtgcgga tattgatatt
    10141 gagattggag gaaatggcat gactgagccg ctcaatcact ccggggccgt cgtctatgcc
    10201 ggtgattttg agatcggcca caaatgtggt gcctggcgtg tgtacccact cggccttcat
    10261 gatgcggtag ccatagttgg ccatcaggtt ggtggcgttg gggcaggtgg tgcggtgtat
    10321 tttgagtgta gcattggagg tgacgtaggc aaaaatatcg tcgccctgca ccggattgca
    10381 acaagtagcc agcgagtaac tgtatcgctc ggcatcttcg ccgttgatca taaggcggcc
    10441 tttgctgagg tcgcgttttt tgctcggcgg gggttctggc ggacgaacct cctcgcccac
    10501 cttgggtatt tccaccagcg tattcccctc cggcatgaac aacttgaaaa tacgctcgat
    10561 ggtgatggcc cccatgtaga tggagtagta gaagtccaca tgagagtcga gatcgaaata
    10621 ccggacgagt ttttcgacga tttcttcgtt gggttccaat ttgagattgc gcaacttccg
    10681 ctcaaaagcc tcgcggccat attcgcccaa cttgcgccgt tcttccttca tggccgaacg
    10741 aatcttggac ttggccttgc cggtgacgac aaacttgagc cagtcttcgc tgggtttttg
    10801 atttttgttg gtaatgacct taacctggtc gccgtttttg agcacacagc ccatcggtac
    10861 cagcttgttg ttgactttca ccgccgtgca atgatacccc acgtcgctat gtatgctgaa
    10921 ggcgaagtcg agcgcagtgg cgcctttggg caatatgcga agctctcctt tgggggtata
    10981 aacgaagatt tcttcgttga agaggttgtt gtctttgaag gtttcgagca cttcaacggc
    11041 attttcgccc gggtcttcca gaatttcccg cacgctgtcg agccactgct caaacacatc
    11101 cttctggttg ttcacgcctt tgtatttcca atgcgccgca aaaccccgct cggcaatctc
    11161 gtccatgcgg ttggtgcgga tttgtacttc cacaaacttg ccttgcggcc cgataacggt
    11221 ggtgtgcaaa gactcgtagc cattcgattt cggcatggta acccagtctt tgaggcgttc
    11281 gggcaggggc ttgtgtacat tggtaacgat ggaataagcc tgccagcagg aaaggcgttc
    11341 gcggtcgagc ggcacgtcca tcacgatgcg gacggcgaag aggtcataga tttcttcaaa
    11401 aggcaccctt ttgctctgta ttttattcca gatggaatag atggatttgg ggcgtccgta
    11461 aacctgataa ggcacgccaa tggcatccag atggggcgtc agcggcttga tgaactcatt
    11521 gatgtagcgg ttgcggtcgc gtttggtatc ggccaaattg cgggcaatgt cgtggtaggc
    11581 ttccggttcg gtcaccttca tacacaggtc cagaaactct gttttcaggc cataaagtcc
    11641 cagcctgtgg gctaatgggg catacacata actggtttcg gcagctattt tgagttgttt
    11701 ttgacgcgtc atggcgccca atgtgcgcat gttgtgcagg cggtcggcca ttttgatgag
    11761 tactacgcgc acatcttcca ccaaggtact gagcactttg agtacatttt cggcctgttg
    11821 actctcggca ttgtaggcgc tgtccaactt agtcaggccg tccaccattt tggcaatctg
    11881 nnnnnnnnnn nnnnaatttt gcccggatgt cttccaattt taccggcgtg tcttctacca
    11941 catcatgcag cagggctgcc gctacggccg taggccccag tccgatttct tcggtgcaga
    12001 tggtagctac tgccaggggg tgcagaatgt atggttcgcc cgacttccgg cgctgtggcc
    12061 tgtgagcttc cactgccagt tcatacgcgt cgcgaatgag cttgcggtcg gcatcttcca
    12121 atggcgtttt gatggtgcgc aagagcttgc ggtacgcatt gtgtaccatt ttgtcttcgg
    12181 gcgacatatt ggaaatggcg gtcattgtgg cagtgtttaa aagggtaatt tgcaaagttg
    12241 agattttttt tggaaaaatc ctcttacctt tgcaacgctt tttgaaaaac gattgttgga
    12301 gggcttgcgc ttcttccggc gtcggcaaaa ttttcagcgg gtgtggcgaa attggtagac
    12361 gtaccagact taggatctgg cgccgcgagg catgggggtt cgagtccctc cacccgcaca
    12421 ggcaaccggt agtttgggtg gagggatgag aagtttatcc cgcaatggcg ggaagtccct
    12481 ccacccgcac catattagta ggtagcaaaa tgtctgaaca gcctcgtcag tggcggttca
    12541 ttccggcttt tcgcaactaa tatgccttct atttcctaaa ttatttatca aacttaatgc
    12601 caactttcca aagagaggcc atcagcgacc tccacttcaa actcaccgcc actctggcgc
    12661 cctccgacta cgaaaccgag tttcatactc aattggaaaa ataccgcaaa gaagccgcca
    12721 tcaagggctt ccggaaaggt aaaacgcctg catcggtggt gcgcaaaatg ttcggcaaat
    12781 cggttttggc cgacatcctc aacaaaaaac tgcaagaggc gatacctgca tacgtgcggg
    12841 aacagcattt agacatgttg ggcgggccta tcccttctga tgagaacgat gccatcgcgc
    12901 tcgacgccaa agaattgaaa gaatacagct tcatctttga aatcggcgtg gcgcctgaat
    12961 ttgacataca gggcacaggg ccggatgcgg aatatgagcg cttggatgtg attgcctccg
    13021 aagagcgcat caacgagcac ctcgaaggac ttcgcaatga ggatcttcgt gcggttgcgg
    13081 taacggaaaa tgtcagtgaa aatggcgagg cgcgccttcg gttcacggaa ttagatggcg
    13141 atgcgccgcg tgaaaacggc attgttacca gctgttcagt acaggtgagg gacatcaaag
    13201 acgatgctct gcgcaatcag atattggcgg ccaaaatcgg agacactttt caaatagccg
    13261 atattttcga ggatttgaat gagaacagcg ctctggtacg gaaccaacta ctgcagtctc
    13321 atgatgacaa tggcattcag atcaatcccg gctttgaggc tgtggtagag gagttgaaca
    13381 gccgcgaacc cgccccgttt gacgaagagt ttttccaata cgcttttggc gaagataccg
    13441 acgtaacgac tgaggcagag gccaaagcac gcatcaaaaa ggatatagaa gaatatctgg
    13501 ccggctcctc acagtctttg ctgcgcgctc aggtgcgtga ccgcatcatg gaattgaacc
    13561 agtttccgct gccggaagcc tttttgaaaa aatggctgct ctactccaat gaggaatgac
    13621 cgaagaagat gtcgaaagcc agtttgacag catcgccaaa tcattccgct ggtccacgat
    13681 cagtcaacgc ctcgccaagc gctttgaaat caaggtgtct gaagaggaag tgcagcaata
    13741 cttcatcaat caggtgcggc aaatgctggg cggatatgcc ggcttgggcg acagctttat
    13801 gttaagcatg gcgcaaagac tcatggagcg cgaagagagt gtgatggagg cttacgaaga
    13861 tatttataac acaaaaatgc tggcggccgt agccggggag gtgaagcaag tgggtaagcc
    13921 tgttacggaa gaagaactcc agcaggctgt acaggcatac aacgccaaac acaagccgca
    13981 gcagcccgaa tctgacgata ttgtggacgc cgaagaggta caacccgagc ctgaagccga
    14041 agccggggaa taatctttga cgcctttcaa cccgaaggca gggaagttgt ttcatattcg
    14101 gttttcataa aaggattgta caaaaacccg aaatgagtat gtttcgcaaa gacgaatttt
    14161 tcaaatatgc cgtaaagcac ctcggcatga gcagctataa ggtggaagac tacgcgcgct
    14221 acaatgtgca gtctatgagc caggtgcaaa acatgacgcc ctacatcatc gaagagcgcc
    14281 aactcaacgc taccgtgctt gacgtgttct cccgactgat gatggaccgt atcattttta
    14341 tgggtacgcc ggtgtacgac gacattgcca acatcgtgca ggcacagttg cttttcctgc
    14401 aatctgccga ccccaagcgc gacatcaaca tgtacatcaa cagccccggc ggctctgtta
    14461 ttgcaggcct cggcatttac gacaccatgc aatttgtgtc gcccgatgtg ggtaccattt
    14521 gcacaggcct ggcggcctct atgggagctg tgttgctggc tgccggcgcc aaaggtaagc
    14581 gtacttgtct ctaccacagc cgcgtgatga ttcaccagcc ttcgggcggc atgcagggcc
    14641 aggtatctga gatggaaatt tcgtacaaac tcatcaagca aatgcaggaa gatttgtaca
    14701 aaatactcag ccaccacacg ggcaaaacgt accaacaaat tgaagaagat tccgaccgcg
    14761 acaaatggct cacctctcag gaagccatga attacggtct cgtggacgaa gtgctgggca
    14821 aaggcgtaga agctgccggc taagttcatt tcatcgaaag cccgcgtccg acgtttattt
    14881 tttttgtctc ggatgtgggc tttcccattt tttgagggcc tccctcggcc ctttttatcc
    14941 aagtccacca aatcatgcgt aaatccagac aaaactacca ctgttcgttt tgcggcaaag
    15001 acaaatctga agcgctgctc ctgattgcag gcatcgaagg ccatatttgt gaagcctgcg
    15061 tagagcaagc cggccaaata gtgcaggaag aattgtacgg caacaaacag gagggcggca
    15121 aaaaagagaa gtccaaatcg ggcgctggtt tttctgtggt aaagccgcag gaaatcaagg
    15181 cctacctcga tcagtacatc attggtcagg acgatgccaa aaaagtgctt tcagtggccg
    15241 tttacaatca ctacaagcgc ctgggtcaac cccgcaaaga cgacgtggac atcgaaaaat
    15301 ccaatattct cttcgtgggc caaaccggca ccggcaaaac cctgctggca aaaaccatcg
    15361 ccaagtttct caatgtgccc tttgctatcg tggatgctac ggtgtttacc gaggcaggct
    15421 atgtgggcga agacgtggag agcatcctca gtcgcctgct tcaggtatgc aactacgatg
    15481 tggccgcagc cgagcgcggc attgtttatt tggatgaaat tgacaaaata gctcgcaaga
    15541 gcgacaaccc ctccatcacc cgcgacgtgt cgggcgaagg cgtgcagcag gcactgctca
    15601 aaatgctgga aggtaccgat gtaaacgtac ccccgcaggg cggccgcaag cacccggaac
    15661 aaaaactcgt taaagtaaat accgaaaaca ttctctttat atgtgggggc gcttttgagg
    15721 gtatagacaa gatcattgcc cgccgcatga acaatcaggt catcgggttc aaagccgacg
    15781 actatgttcg ggtggacagc gacaatatgc ttcaatacat caatcatcag gatgtaaagc
    15841 attatggtct gattcccgaa ttggtgggtc gccttcctgt attagcctac ctcaatccgc
    15901 tcgatcagga aaccctgcgg cgcattttgc tggagcctaa aaactctctc atccgccagt
    15961 acgagcgctt gtttgaaatt gagggcattc aactgagctt cgaaccggat gccattgagt
    16021 tcatcgctga aaaagccctc gaatttgcgt tcggtgcgcg cggcttgcgc tctatttgcg
    16081 aagccattat gacggatgcc atgtttgagt tgccttctca aaatgaagtg cgccacctcc
    16141 acattacccg cgcttatgcc gcagaaaaac tctcccgttc caatctgggc aagttgaggg
    16201 ctaaagcggc gtaggagcat agaaggggag ggtgttttta gattcgtcac caaattgatc
    16261 cctgaggcta tgaatcgaat catcgcgttg ttgctgtttt tgtctgtcgg aatggttcaa
    16321 tccattgtcg gccagcaaat tatcatgtcg gaagacattc ccctgcgcaa tgatgtctcc
    16381 tacgaattgc tgggcgagtt caaggggcgg gtactgcttt tcagggatca gatcagtaaa
    16441 tatgaagtgc agggttttga ccaacgcctg cgccccagtt ggtccaaaat actgacgctt
    16501 gataagaaaa accccaaagt catcggcacc acgcacagcc gaaacgactt cacgctcatc
    16561 taccattacc gggatcaggg gaacaccatc ctcaaggccg ccaaattcga ccccggcgcc
    16621 aacatgatag actctgcaac gatcaaaaac ctcggcagcg ttttttttac acccgacttt
    16681 gaaatcacct actccgaaga ccgcagcaag atacttgtat attatattga gcgacaagcc
    16741 gaattacatg cttatgtttt tgatgcagtg ggatttaagt tgctgtggga aaaaaccatc
    16801 gtgccgagcg ggttctcttt ttttgaagat tttctccagg tcgtgcttga caatgacggc
    16861 aatatgtgcc tcatttttga aaaagataaa tttgtggccc gccgagacgg tcatcactac
    16921 catatcatcg gagttattgg cgccgaagac cgccttgttc agtttcaggc tcctttggaa
    16981 ggcaagctca cctacgacgt tctgtttact tatgacaacc tcaacgaccg gctcgtagct
    17041 gccggtttgt actcagataa ggccggcgac cgcgctaccg ggtattttat attaaagata
    17101 gacccactgc aacgggaatc gccttttcta aggtttgaat ccttcagcga tcttttcgtt
    17161 tccaatctca ttggcaagga agtagagaaa aataaaggag tgacggagtt gagcgttcgg
    17221 gaaattgtgc tgcgacgcga cggcggcgtg ttgatgattg tagaacgtat taaagaatat
    17281 gagcgtcggc tgggcgcccc caatagggtg agctttgacg gcgtgggccg gttcatcgtg
    17341 gattattatt acgaagacat cgttgcgctt tccattcatc ccgatggcca ggcacattgg
    17401 gaaaccatat tgcccaaaaa gcagtattcg caagacgacg ggggcgtgta ctcttcctat
    17461 tttctgttca aaacccctga aaatctgaga tttttattca acgatgaaat tcggtttgaa
    17521 aacacagtaa gcgaataccg gctcaatggc aacggggcat ttgaacgtcg cagtctcctc
    17581 aatacagaaa aattggaagt acgcctgcgt tttcgcgacg ccattcaggt gagcgccgat
    17641 gccttcgtgg tgcccagcga acgccgcaac aggcttcgtc tggtcaaagt gatctttgag
    17701 gagtaggcat aagaaaattt gcatcattca aaaaaatatt tggctgttta acacagaaca
    17761 attacctttg cgccactttg ctaaaaaggg tactaaaaat ctgaatatca atggacgcga
    17821 tcaagtttgt acacgaacaa atgtctccgg aacggaactt ccctgaattt tttccgggcg
    17881 acaacataac catcaattat aaaatcatcg aaggcgacaa ggagcgcgta caggctttcc
    17941 gaggcgacgt catccagata agcggccaca acggcacgcg caccttcacc gttcgcaaaa
    18001 tgtcaaatgg cgtaggtgtg gagcgtattt tcccgttctc tgctcccaac atcgttgata
    18061 ttcaggtgct caagcgcggt aaagtgcgtc gtgcccgtct gttctacctc cgcgatctcg
    18121 taggcaagaa agcccgtatc aaggagagaa aatttgttgc caagaagtaa tttattcttg
    18181 ttcaggcact tgctgccgat acgccgtttt ttcggcatgg gtcatctcgg atgcacatat
    18241 tcgggatgac tttttttgtg caatccccta cctttgtccc ggttttcaaa atcaacacct
    18301 tctttgacaa atagtgtggg aacagattta gcggaaggca ggacgactgt aa
//
LOCUS       DAORLF010000034        18274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1636_length_18274_cov_1.936824, whole genome shotgun sequence.
ACCESSION   DAORLF010000034 DAORLF010000000
VERSION     DAORLF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_18274_cov_1.936824"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>299)
                     /locus_tag="PK198_04950"
     CDS             complement(<1..>299)
                     /locus_tag="PK198_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38113.1"
                     /translation="GRPWQCELFVKFYNDARELKGQVVRLQQISKSDEFIKLTAGWGS
                     NVKGSWRKDRYTAELIFMDRLLAVVPFEVGDEIEQGVAPALLPDQQAPIIMGIEE"
     assembly_gap    300..388
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<389..>681)
                     /locus_tag="PK198_04955"
     CDS             complement(<389..>681)
                     /locus_tag="PK198_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38114.1"
                     /translation="FSLKRGRREICTLDLKGKKISKLDHIVYIREGWGNKTEGAFWKR
                     GTYFWEAWVDGQKVGAKYFYVEDPGKDFYSINDAMYADVHSLRLYEGPYDDVA"
     assembly_gap    682..756
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(973..>1596)
                     /locus_tag="PK198_04960"
     CDS             complement(973..>1596)
                     /locus_tag="PK198_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38115.1"
                     /translation="PKALLIRKLWGHIAPQLWAPLLGVKPTELPRQIERGEWSGALTE
                     ALTDAIVLFRSSEQADALVRYWRYKFTDELPGGWERLMPLLSEGAFADIVREALSGSA
                     TLLPDRSLAYYLLLNKTLPWPADIAAQTIGGLRQWLAQSTAPDWSAMHYRALLKTAAY
                     RCPVILLPKLDRDWPAANGKVWYYWENDVRLFLRVLTFRKEMTDSFF"
     assembly_gap    1597..1606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1607..>2138)
                     /locus_tag="PK198_04965"
     CDS             complement(<1607..>2138)
                     /locus_tag="PK198_04965"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HRF38116.1"
                     /translation="LPLVLEWLAKEKSRFEEARPLLGARGRWLMAQLPEWQPLLKETD
                     PKSWQIASLEERKKILLQVRKTNPEAGLQQLRSTWAEDGWKERHELLKRLETGLSLSD
                     ESFLETCLDDKRREIRQTAARLLALLPGSALSERLQSYARSMLKFSLTGAWQLSPPEQ
                     PPAAWQRDGIDGGGAGT"
     assembly_gap    2139..2172
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2173..2477)
                     /locus_tag="PK198_04970"
     CDS             complement(<2173..2477)
                     /locus_tag="PK198_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38117.1"
                     /translation="MENQLLTAAILGLDRQELPAEAKAVALPHDDEASALLRAWARRQ
                     LTDKAAALLPDAPPMPAPDNAGEQAAGEEAARFVQQILSGPYREAWEEMLDLLRKTG"
     gene            complement(2549..2821)
                     /locus_tag="PK198_04975"
     CDS             complement(2549..2821)
                     /locus_tag="PK198_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF38118.1"
                     /translation="MNKGDLISKIAGDAGITKAQAGDALNAVLDGIAGALKDGDKVTL
                     IGFGTFSVNKRDARTGRNPKTNAKIEIPAKTVVKFKPGKELADSIG"
     gene            3047..3229
                     /locus_tag="PK198_04980"
     CDS             3047..3229
                     /locus_tag="PK198_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38119.1"
                     /translation="MKKTIYFLLVLFLLGTAAAACSPKVGCPVTESARPNTNRKGELS
                     TKRGKSQLFPKNMRRG"
     gene            3277..>5559
                     /locus_tag="PK198_04985"
     CDS             3277..>5559
                     /locus_tag="PK198_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38120.1"
                     /translation="MLLGTAQAQSVWSNQRSRLLEIRNGCYELDSLTVIPASVVLKNP
                     ATGERVGSLLYRIADNQICFSRSDTSALSLRIDFRVLPYRLGAALFRIDSSSLVPPED
                     RILRTATYNPYAASGPAGLGFGGLQYNGSFARGLSFGNSQDLVLNSNFNLQMAGRLGD
                     GLEIVAAITDENIPLQPEGNTRQLREFDRVFVQLSKGKGKLVAGDYDLLRPQSYFMNY
                     FKRLQGLSFRIESQDAGKGLWRGDAALAISRGQFARNLIAQQEGNQGPYRLRGNAGEL
                     FIIVLAGTEKVWLDGRLMERGIETDYVIDYNRGEITFTNKRLITKDSRIIVEFEYADQ
                     GYLRSLYAFNAEYVRPKSRLYANVFSQQDSRTSTGISPLSAEQRSVLRNAGDDFTTAV
                     SSGIDSIAEFLPFRAQYRLTDTLSICGERDTVLVFSTDPALARYTARFSFVGQGRGNY
                     VLDPTLTANERVYRWVEPDPLNCLPRGDYEPVVQLTAPQQQQLFTLGAEQRFSKQSLW
                     RTELALSRKDLNRFSDLDNKDDLGTALFSSLRHQLPRGKNATDTSGTRLELAADYEWV
                     QRHFKALNPYRSPEFQRDWSLTDIQGLGNVQAATENLGRANLAFRRSGWGVLQYGFAA
                     FLRGSVYRGSRHTAQLDLLRKGWEMKAEGSWLEAVNAGQRNSFVRPRLRLSRSFEKLG
                     GWRAGIYAERERNERRLLSADTLEANSFFYDLQKAFIESPAGKENFVLGAHFQRRQDY
                     APAGADFVRNTRATELNLNGS"
     assembly_gap    5560..5774
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5775..6774
                     /locus_tag="PK198_04990"
     CDS             <5775..6774
                     /locus_tag="PK198_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38121.1"
                     /translation="IRVRQGEGTHIWLDSLNNNDGIIQPNEMEPAPFSDLADFVRVPT
                     VSNEFIRADYVNLLQSWQLEPRAIWFNTKGWKQFLSKFALQSSIKTGQRTRSAPGVQT
                     WNPLQWNIPDTALVAGNAATRHLLFFNRAHPKWDLQAGLLDVWNKTVQTTGFEDRRNR
                     EQFLRGRLNLSKPFSALATATWSRRSSDSEFFNNRDFDIAGFALEPQLNWQPARQFRL
                     TARYKFQTDFNQLPGGGEQSERHDLSAETAFNKAGAWALRSRFSYIRVAFDGAANSPV
                     GFAILNGLQRGNNWLWNIGLDRQLAKNLQLSIQYEGRQTGAGRMVHVGRASVGAVF"
     gene            complement(6808..7935)
                     /locus_tag="PK198_04995"
     CDS             complement(6808..7935)
                     /locus_tag="PK198_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRF38122.1"
                     /translation="MAKKLRRNLLLVCEGSVTEPDYFALLKQKSLEIGVWDAITIEPK
                     PRSEQDDEPVKAFPAHKSPRPRRQLKAVPVEEDADETERRFNHRQTPVNFVKAARNGL
                     KDNVFEEVWAVFDRDGHPGHEEAFQLAEERIEGKKVNIAFSSIAFEHWILLHFERNLT
                     AFVKSECKSENGRYLNCGSDQHDGDCRGERCVAGRLRMNGYLEGSTKHFDESNRAFFK
                     NLIEPTMFQTACTNAAWLRNKVSHDPSKPFLTNPFTNVDDICRRLLGTDHETVEWANL
                     GEPKIWRTLVLLLEIENDVLKCSISNEGRHSRLFNGTDISAFMLQNGEQIVLTPTPES
                     SVLISVGETKTFHFTGRPDWQSILLGFQTGSHYLFIDPAVE"
     gene            complement(7928..8311)
                     /locus_tag="PK198_05000"
                     /pseudo
     CDS             complement(7928..8311)
                     /locus_tag="PK198_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(8327..9103)
                     /locus_tag="PK198_05005"
     CDS             complement(8327..9103)
                     /locus_tag="PK198_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38123.1"
                     /translation="MALPGNDTVRLLKSAVIYGANASGKSNLIRALGAMKELVVLRRA
                     SAGSSIRQYDPFAFSPETPDKPTVFELVFVLNNVKFKYRIVFEKMQIVEEVMEHFPKG
                     QTKQIFKRIAQSDGALHKVKLAKDYGNKELNVFSNHSALSTFGREHPHELLTQVFIYF
                     RNITISYFDRKLSTSSGYYERLTQAEEQPAFTKLQKLIRAADTQIESLGILKEEVQMP
                     VTAPDEAGKTLVPMRKVSFLSVHNVFDASSQKTGIAAIDL"
     assembly_gap    9156..9167
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9474..>9522)
                     /locus_tag="PK198_05010"
     CDS             complement(9474..>9522)
                     /locus_tag="PK198_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38124.1"
                     /translation="LAWQRTVAFFREKLK"
     assembly_gap    9523..9557
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9558..10382)
                     /locus_tag="PK198_05015"
     CDS             complement(<9558..10382)
                     /locus_tag="PK198_05015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRF38125.1"
                     /translation="MKKINKADIRQEVFDLYDDYAHNRLDRRDFMEKLSVFAVGGLTV
                     PALLGFIMPDYKGTLQVAPNDPRLNSEYVEYDSPKGGGKIKALLSRPKGNTGKLPGIV
                     VVHENRGLNPHIEDVARRAALEGFISIGPDALTPLGGYPGTDDAGRELQAKRDREEML
                     QDFIAAFEYLKTHPECNGKVGVVGFCFGGWVSNMMAVRIPDLMAAVPFYGGQAPAEEV
                     PSIKAPLLLHFAGLDTRVNEGWPAYAAALQANDKEYSAHLYPGVNHGFHNDSTPRYD"
     assembly_gap    10477..10515
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10516..11042)
                     /locus_tag="PK198_05020"
     CDS             complement(<10516..11042)
                     /locus_tag="PK198_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38126.1"
                     /translation="MKKIFPFCIVSFLFALQTPAQTRAARRRTSAGTTDSLRIAEINR
                     TYWAEVSRSVKKGDFEGYKATCQKNAVLVATAGKNKRSEPMTAALARWKRGFMDTKEG
                     KQIDHVSFRFSQRIGGETTAYETGIFYFTSHDATGKLLSESYTHLEALLVKKGRKWVC
                     LMEYQKSAATKAEWDA"
     gene            complement(11224..>12053)
                     /locus_tag="PK198_05025"
     CDS             complement(11224..>12053)
                     /locus_tag="PK198_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRF38127.1"
                     /translation="WALTGPFQHHITGCVGWSFWQYYLVTKDKEWLREKGYPMLKEVA
                     DFWAGRVERNGPGKYDINNVIGANEWQENIDNNAFTNGMAVTVLQYAAAAAQELGLTP
                     NPDWLHVAQNIPILKFPDGTTRENATYDGVTIKQADVNLLAFPLKIVTKEADVRKDLA
                     YYEPRMAPDGPAMGHSVLAALHARLGNSEKAYQMFVKSYKPNEVPPFGVLAETAGGTN
                     PYFATGAGGMLQAVLMGIGGLDISEQGIVQLKTKLPSQWKSLTIKGVGKEKKTFTIR"
     assembly_gap    12054..12191
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12192..13265)
                     /locus_tag="PK198_05030"
     CDS             complement(<12192..13265)
                     /locus_tag="PK198_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRF38128.1"
                     /translation="MKNRILLALLFGVVQLSAQQDLSQSPWHIQARDIDPGRYFGITV
                     ANGMIGLVSSPEPMRVQDVVLNGAYDYYQRGRVSNILKTFNHLNMNLDVDGRRIGRRD
                     IANYAQTLNMQTATLLTTFDVGDQLSVRHSTMALRHLPYTALSVVEITAKKDVTITPM
                     SVIEAPTHLADVRNYYSEIDRPHVLIPLLTSVGKSPSGRYTVAASTSFIFEEPHGQEP
                     KIIHEDWDYNMHLAKFYKKLKAGETYRFSLVGSTVSSHTYNDPHNEAERLTIFTKLEG
                     RERLLRRHEAEWNKLWQSDIVVEGDLESQRAIRSALYHLYSFAREGTALSLSPMGLSG
                     LGYNGHVFWDTELFIIPPLILTQP"
     gene            complement(13423..>14016)
                     /locus_tag="PK198_05035"
     CDS             complement(13423..>14016)
                     /locus_tag="PK198_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38129.1"
                     /translation="FTAEDACGNRSTRSAMIEVRDTTPPVWTQLPTPIVLQCGEDLWG
                     AFLPWLANSGGGMVEDDCGTSSITYFYTEQVDCLDPDTLHVVFSGRDACGNVSEITIP
                     VVVLRPTDIYNPAFEISSIRVFPNPARESVQIQWQGAENESFRLQLWNASGHVMTTVE
                     GRGHALELSVAAYSSGVYFFAIETAGGKKGYGRVVRM"
     assembly_gap    14017..14050
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14257..16704)
                     /locus_tag="PK198_05040"
     CDS             complement(14257..16704)
                     /locus_tag="PK198_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38130.1"
                     /translation="MFQQPVPSPKLIPVPSGGYYINLGIDQDVVGGRFQWYNRGSLPY
                     FMGETETNQIFVETFNPQDSFIVYISHPDLPGLTEMSTPTFYIRDAYPVVKGRLVHDT
                     NSNCIPEESEESLANWDVRMVGASTYYGRTNAQGEFHFVMDTGQYQLEVLLPNNQWSL
                     CPGIYNIQVTYLDTLELSIPVQSFTFCPELQVNVGIQNPFYRRCFSNKYAIGYCNKGT
                     APEGNARVEFWLDEWIVLDSASVPIVHLGGGHYVSDIGNLDVGQCKTFSVYVSIDCDA
                     PLNAVQCFRAHIYPDTLCNAPSGTWPGATMDVSGRCENDQAHLLFRNTGHQAPADSLE
                     YRIYQNGVLVEAARYRLAAGDSLVFHRPGGNNAWRLETDQAPGHPCSVLPSVFVEGCG
                     PGLPGQAVTSYALQFPDDSNCPFEDKVCFPITGSYDPNDKTAFPVGYGPDHLIEPGTD
                     IEYLVRFQNVGNDTAFHVFVRDTLSAFLDPATVEVTGFSHPYQLNVDSTGALEFAFYH
                     IHLTDTITDEPGSHGYIRFRVSQKSGNIIGAIIRNQAAIYFDFNAPIITNLVFHTLGV
                     DFVPDALAPVWETPPADLVFNCHRNMDVDALIQQWLNNAGGALVSDHSMPITLSNDYT
                     ALSGGCNGFSGTATVTFTATDRWGNSSSSIATLSAMDTIPPVWEILPQSVEIACSEDL
                     PTRLADWLLDNGGGEVSDACGTVAVTYAYELSGACTDSTLVTFIAVDECGNTAQIQAY
                     FIVSNSLPPAITAPASDRRAGFARPHPAMAAGTWRRTGRGRLFFGELAPRFRPADARL
                     RQYERNKRHLHRGGCLW"
     assembly_gap    17011..17026
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17027..>17380)
                     /locus_tag="PK198_05045"
     CDS             complement(<17027..>17380)
                     /locus_tag="PK198_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38131.1"
                     /translation="NLPDFSHLPQLKTIEVSDSEVSGNLPLFQHCPLLRQIFFSSTQL
                     SGEIPNYQLPYLDVLEISSSRILGQLPNFDGLPNLKSLYLSDNHLIGRFPKIENCPGV
                     EHIYAQNNNFSGPIPS"
     assembly_gap    17381..17417
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17418..17731)
                     /locus_tag="PK198_05050"
     CDS             complement(<17418..17731)
                     /locus_tag="PK198_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38132.1"
                     /translation="MTDTIPDFTNLPNLRSLKIGGGNSTGTIPDFAYLPFLSTMSLVG
                     NFSGDIPDFSNIPLLNSLEIGRVIGSIPDFSFIPNLRKLIVTGSGNLPDFSHIPEVSE
                     LR"
     assembly_gap    17844..17894
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17895..18262)
                     /locus_tag="PK198_05055"
     CDS             complement(<17895..18262)
                     /locus_tag="PK198_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38133.1"
                     /translation="MKKSLYPLLLLGAVLSTFHTVHAQCDRTADSLELVQMYNAWAGP
                     EWTNTWDLSEPLDSFYGTTLNAQGCVTGLDLFKNNLQGEIFDINLPALSSLILSANYE
                     LGGSLPNFSQIPQIHTIKLYG"
BASE COUNT         4011 a         4589 c         4601 g         4288 t
ORIGIN      
        1 tcttcttcta tgcccatgat gatcggggcc tgctgatcgg gcagcaatgc gggcgctacg
       61 ccttgttcta tttcatcgcc tacctcaaaa ggcactacgg ccagcagccg gtccatgaag
      121 atcagttctg cggtatagcg gtctttgcgc caggagcctt ttacgttgga cccccagcct
      181 gcggtgagtt tgatgaattc gtcggacttg cttatttgct gcaagcgcac tacctggccc
      241 ttgagttcgc gggcatcgtt gtaaaatttg acaaacaact cgcattgcca ggggcggccn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggccacatca tcgtaaggcc cctcgtacag
      421 ccggagcgag tgtacgtcgg catacatggc atcgttgatg gagtagaaat ccttgccggg
      481 atcttctaca taaaaatact tggcgcccac tttctggccg tccacccagg cttcccaaaa
      541 ataggtgccg cgcttccaga aagcgccctc ggttttgttg ccccagcctt cccggatgta
      601 aacgatgtgg tcgagtttgc tgattttttt gcctttgaga tcgagcgtgc agatttcccg
      661 ccggccgcgc ttgagcgaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntata tagctggtct cgtaccggtc
      781 gaacacttgc cggtacttct tcttgttgtc ggccagccat tcggtggagg catagacctt
      841 gagttctctg aacttgtact tatgcgatat gggcgcgatg aagctctctt cctgttccat
      901 tattggtagt tgaatcaatg gacaataaag ataatggagt ttccggtttt gcaagccgtt
      961 cggggctttt ttttagaaaa aactatccgt catttctttt ctgaaagtca gtacccgcag
     1021 gaaaagccgc acatcgtttt cccagtaata ccataccttg ccgttggctg cgggccagtc
     1081 tctatccaat ttgggcagta aaataacggg gcagcggtag gccgccgttt tcagcaaggc
     1141 ccggtagtgc atggcgctcc agtcgggcgc ggtgctttgc gccagccatt gccgcagccc
     1201 tccgatggtc tgggctgcta tgtcggcagg ccagggaagc gttttgttga gcaataaata
     1261 ataggctaaa ctgcggtcgg gtaggagcgt ggccgagccg gacagtgcct cccgaacgat
     1321 gtcggcaaaa gcgccttccg acaacaaggg catcaggcgc tcccagccgc cgggcagttc
     1381 gtctgtgaat ttgtagcgcc agtaccgcac taaagcatct gcctgttcgc tgcttcggaa
     1441 aagaacaatg gcgtctgtca gcgcttccgt cagtgcgccc gaccactccc cgcgctcaat
     1501 ttgccgcggc agctctgtcg gcttcacgcc caacaacggc gcccaaagct gcggcgcaat
     1561 gtgcccccaa agttttcgta tcaacagggc cttgggnnnn nnnnnncggt gcctgctccg
     1621 ccgccgtcta taccatcgcg ttgccaggct gcgggcggct gttccggcgg cgataattgc
     1681 caggcgcctg tgagcgaaaa tttcagcatg gagcgggcat aactttgcag gcgctccgac
     1741 aaggcggaac ccggcagcag tgccagcagt cgggcggcgg tttggcgaat ctcccgccgt
     1801 ttgtcatcca aacaagtttc cagaaaggat tcatccgaca gggataagcc ggtttccagc
     1861 cttttcagca actcgtgacg ctctttccag ccgtcttcgg cccaggtgga gcggagttgt
     1921 tgcagtcccg cttccgggtt tgtttttcgg acttgcagta agattttttt tctttcttcc
     1981 aaagaggcaa tttgccagga tttagggtca gtttctttta aaagaggctg ccattcaggc
     2041 aattgtgcca taagccagcg cccgcgggcg ccgagcaaag gccgcgcctc ctcgaaccgg
     2101 gatttttctt tggccaacca ttccagcacc aagggcagnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nntggcctgt tttcctcaaa aggtccagca tttcttccca tgcctcgcgg
     2221 taggggccgc tgaggatttg ttgtacgaaa cgggcggcct cttctccggc ggcctgctcc
     2281 ccggcattgt caggggcggg catgggaggc gcatccggca gcagtgcagc ggctttgtcg
     2341 gtcagttgtc ggcgcgccca ggctctcagt agcgcggagg cttcgtcgtc gtggggcaat
     2401 gcgacggctt tggcttcggc gggcaattcc tgccggtcga ggcccagtat agcggcggtg
     2461 aggagctggt tttccatctg gaaacgaaaa agagcaaccc gaaacacgcc cggattgccc
     2521 ttttgtaagt cttttgagag actcaggctt agccgataga gtcggccagt tctttgcccg
     2581 gtttgaactt cacaaccgtt ttggcaggga tttcgatttt agcgttcgtt ttcgggttac
     2641 gaccggtacg agcgtcacgc ttgtttaccg agaaagtgcc aaagccgatg agggttactt
     2701 tgtcgccatc tttgagggcg cctgcgatac cgtccaatac ggcattgaga gcgtcgcctg
     2761 cctgggcctt agtgatacct gcatcgccgg cgattttgct gatcaaatct cctttgttca
     2821 tttcaaaatg agtttttgtg aaataatggt gacgcaaaat tagcgtttac tttggtaccg
     2881 tcaacttttt ttttctgaaa aaaaatgaaa ccccttgtat ttacaggggt tccggcccgt
     2941 gaggtacatg ataggggaga tgcaaagccg ggaaaaacga gttaattttg cgattcgttc
     3001 tgtctaattc ttgtcaatct tcaacacaat caattgatta tcaataatga aaaagaccat
     3061 ttatttcttg ctcgttttgt ttttattggg aaccgccgct gctgcctgtt cgcccaaagt
     3121 gggctgcccg gtcaccgaaa gcgcccgtcc taatacgaac cggaaaggcg aactttctac
     3181 caaacgcggt aaatctcagt tatttccaaa gaacatgcgg cggggttgag cctccaattt
     3241 ctgatattcc gactcgtatt atctgcaaca tgctgcatgt tgctcggtac tgcgcaggcg
     3301 caatcggtct ggtcgaatca gcgcagccga ctgctggaaa tcagaaacgg ctgttatgaa
     3361 ttggacagcc tcacggtgat tcctgcttcg gtggttttga aaaatcccgc cactggagag
     3421 cgcgttggca gcttgttgta tcgcatcgcc gacaaccaga tttgcttctc ccgctccgac
     3481 acttccgcgc tgtcgcttcg cattgatttt cgcgtgctgc cctaccgcct gggcgcggcg
     3541 ctgtttcgca tagacagcag cagcttagta cctcccgaag accgcatcct tcgcaccgca
     3601 acgtataatc cttatgccgc atcggggccg gccgggttgg gttttggggg cttgcaatac
     3661 aacggcagtt ttgcgcgcgg cctttccttc ggcaacagcc aggatttggt gctcaattcc
     3721 aatttcaacc tgcaaatggc aggccgcctc ggcgacgggt tggaaatcgt ggccgccatc
     3781 accgacgaaa acattcccct gcaaccggaa ggcaacaccc gacaactgcg cgaattcgac
     3841 cgcgtttttg tgcaactcag caaaggcaag ggcaagcttg tggccggcga ctacgacctg
     3901 ctgcgaccgc agagttattt tatgaattat ttcaagcggc tccagggctt gtcctttcgc
     3961 atagaatcgc aggatgccgg caaagggctg tggcgcggag atgcggctct ggccatctcg
     4021 cgggggcagt ttgcccgcaa tctgattgcg caacaggaag gcaatcaggg gccgtaccgg
     4081 ctgcggggca atgccggcga actgttcatc atcgtgctgg cgggcaccga aaaggtatgg
     4141 ctcgacggcc gcctgatgga gcgcggcatt gaaacggatt acgtcattga ctacaatcgc
     4201 ggcgagatca ccttcaccaa caaacgcctc attaccaaag acagccgcat cattgtggaa
     4261 tttgagtacg ccgatcaggg ctacctgcgc agcctgtatg ctttcaacgc cgaatatgtg
     4321 cgccccaaaa gtcgccttta cgccaatgtt ttcagtcagc aggacagccg cacctccacc
     4381 ggcatcagcc cgctcagcgc ggaacaacgc agcgtcctcc gcaatgccgg cgacgatttt
     4441 accaccgccg tcagttccgg catagacagc attgcagagt ttttgccctt tcgggcgcaa
     4501 taccgcctga ccgataccct aagcatctgc ggggaacgtg atactgtgct ggttttcagc
     4561 accgatccgg cattggcccg atacaccgcg cggttttcat ttgtggggca ggggcgcgga
     4621 aattatgtgc ttgatcccac cctgaccgcc aacgaacgcg tgtatcgctg ggtagaaccc
     4681 gacccgctca actgcctccc gcgcggcgac tacgaaccgg tggtacaact cacggcgccc
     4741 caacaacagc aactctttac cctcggcgcc gagcagcgct tttccaaaca atccctgtgg
     4801 cgcaccgaat tggccctcag ccgcaaagac ctcaatcgct tctccgattt agataacaag
     4861 gacgacctcg gcacggcgct gttttcttcg ctccggcatc agttgccccg cggcaaaaat
     4921 gctaccgaca cctccggaac ccgcctcgaa ctggccgccg attacgagtg ggtgcagcgc
     4981 catttcaagg cattgaaccc ttaccgttcg ccggaatttc agcgcgactg gagcctgacc
     5041 gacattcagg gcttgggcaa tgtgcaggcg gctaccgaaa atctggggcg ggccaatttg
     5101 gcgtttcggc gttccggctg gggcgttttg cagtatggtt tcgcggcctt tttgcgcggc
     5161 agcgtctatc gcggatcgcg gcacacggcg caattggacc tgctgcgcaa gggctgggag
     5221 atgaaagcag agggcagttg gttagaggcc gtcaatgccg ggcagcgcaa cagctttgtg
     5281 cggccgcgcc ttcgtttgag ccgtagtttt gaaaaattag gcggctggcg ggccggcatt
     5341 tatgcagaac gcgagcgcaa cgaacgccgc ctgctgagcg ccgacaccct ggaggccaac
     5401 agtttttttt acgatctcca aaaagcattt atcgaaagcc cggccgggaa agaaaacttc
     5461 gtactcggtg cgcattttca acggcggcaa gattacgcgc ccgccggcgc cgattttgtg
     5521 cgcaataccc gcgccaccga actcaacctc aacggcagcn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnncattcg ggtgcggcag ggcgaaggca cccacatctg gctcgactcg
     5821 ctgaacaaca acgacggcat catacagccc aacgaaatgg agcctgcgcc gttttccgat
     5881 ctggccgatt ttgtgcgcgt gcccacggtg agcaacgaat tcattcgcgc cgattatgta
     5941 aatttgctgc aaagctggca gttggagccg cgcgcaatat ggtttaatac caaaggttgg
     6001 aagcagtttt tgagcaaatt tgccctccag tcttccatca aaacggggca gcgcacccga
     6061 tccgctcccg gcgtacaaac ctggaacccg ctgcaatgga acatccccga cacggctttg
     6121 gtggcaggta atgcagccac gcggcacctc ttgtttttca atcgggcgca ccccaaatgg
     6181 gatttgcagg caggcctttt agacgtgtgg aacaaaaccg tgcaaaccac cggatttgaa
     6241 gaccgccgca accgggagca gtttttgagg ggccgcctca atctgtcgaa gcccttcagc
     6301 gctttggcca cagccacctg gagccggcgc agcagcgatt cggagttttt caacaaccgc
     6361 gatttcgata ttgccggttt tgcactggaa ccgcaactca attggcagcc cgcgcggcag
     6421 ttccggctca cggcccgcta caagtttcaa acagatttca accaactgcc cggcggcggc
     6481 gagcagtcgg agcggcacga cctcagcgcg gaaacagcct tcaacaaagc gggagcctgg
     6541 gctttgcgca gccggttttc gtacatccgg gtggcatttg atggggctgc caattcaccg
     6601 gtgggattcg ccatcctcaa tggcctgcaa cgcggtaaca actggttgtg gaacatcggc
     6661 ttagaccggc aattggccaa aaatctccaa ctcagcattc aatacgaggg gcgacaaacc
     6721 ggcgcgggcc ggatggtgca tgtgggcagg gcgagcgtag gggcggtgtt ttgagggggg
     6781 gggattatct gagcagtgaa aaccagatca ctcgactgcg gggtcaatga acaaataatg
     6841 gctgcctgtc tggaatccca aaagtatgct ttgccaatct ggcctgcctg tgaaatgaaa
     6901 ggttttcgtc tcgcccactg aaatcagtac agaagattcg ggtgttgggg ttaaaacaat
     6961 ctgctcccca ttttgaagca taaacgctga aatatccgtg ccgttaaaaa gccttgaatg
     7021 gcgcccctcg tttgaaatgg aacatttcaa aacatcattt tctatttcca gcagcaaaac
     7081 caaagtacgc cagattttgg gttctcccag atttgcccac tccactgttt catggtcggt
     7141 tcctaaaagc cttctgcaaa tatcatccac attggtaaac ggattggtca aaaatggttt
     7201 gctggggtca tgggaaactt tgtttctcag ccatgcagcg ttggtgcaag ccgtttggaa
     7261 catggtcggt tcaatcaggt ttttaaaaaa tgctcgattt gattcgtcaa aatgtttagt
     7321 tgaaccttcc agatacccat tcatacgcaa cctgcctgcc acgcagcgct cgccccgaca
     7381 atcaccgtcg tgttggtcgg agccgcaatt caagtagcgg ccattctcgc ttttacattc
     7441 tgattttaca aatgctgtca gatttctctc aaaatggagt aaaatccaat gttcaaaggc
     7501 gatgcttgaa aaagcgatgt ttactttttt accttcaata cgttcttcgg caagttgaaa
     7561 ggcttcctca tgtcccgggt gcccatctcg gtcgaaaacg gcccaaacct cttcaaatac
     7621 attgtctttc aggccgtttc gggcggcttt tacaaaattg acgggcgttt gtctgtggtt
     7681 gaatctacgc tccgtttcat ctgcatcttc ttctacgggg acggctttca attgtctgcg
     7741 cggacgtgga gatttatgag cggggaacgc tttgaccggt tcatcgtctt gttcgcttct
     7801 gggtttaggc tcgatggtaa tggcatccca aacacctatt tcgagcgatt tttgcttcag
     7861 cagagcaaaa tagtctggtt cagtcacact gccctcgcag accagcaata ggttcctgcg
     7921 gagtttttta gccatgactg aaaatagttt ctatgtcctt gagattaggc aaaccaccga
     7981 atttcccagc catgtaccat ttgtcaaaag gcacatcttc ccgaacaccc tcaaaatcct
     8041 gggcggagta cagctcggtt tcccccaact tgttcttctc tgcaaaccag atttggtcgg
     8101 tacggaataa atcgcggtca agcaaggtaa cctcgtgcgt tgcaaaaatg agctgagctt
     8161 tacccggatt ggattgcgga ttggtgaaca gcatcacgag gaattttgcc aatttgggat
     8221 gcaggctgtt gtccatttca tctacaacga gtacgccgcc tttttctaac ttttctaaaa
     8281 tcaggctgcc tcgccaaaga agcgctttgg tgccttcgga ttcctcctac aagtctattg
     8341 ccgctatacc tgttttttgg ctggaggcat caaacacatt gtgtacgctg agaaaactga
     8401 ctttccgcat tggaacaagc gttttgccgg cttcatcagg ggcggtaaca ggcatttgca
     8461 cttcttcctt caaaattcca agactttcta tttgggtgtc ggccgcccgg atcaatttct
     8521 gtaatttggt aaaagcaggc tgctcttcgg cctgggtcaa tctttcatag tagcccgaac
     8581 ttgtggatag ctttctgtca aaatagctga tggttatatt tctgaaataa atgaatacct
     8641 gtgtaagcaa ttcatgcggg tgctcccgac caaacgttga caaagcactg tgatttgaaa
     8701 aaacattcaa ttctttattg ccataatcct ttgccaattt aactttatgc aaagctccat
     8761 cagattgtgc tatccttttg aaaatctgct tggtctgccc ttttgggaaa tgctccatta
     8821 cctcctctac tatttgcatt ttttcaaaaa ctatcctgta tttgaatttt acgttgttca
     8881 atacaaagac caattcaaac acagtaggct tatctggggt ctctggggag aatgcaaacg
     8941 ggtcatattg cctgatggac gaaccggcgg atgctcgtcg tagtactacc aactccttca
     9001 ttgcccccaa tgcccgaatc aaattgcttt ttccagatgc atttgccccg taaatcaccg
     9061 ccgacttgag cagccgaacc gtatcgttgc cgggcagcgc catagaaaac acattgtcac
     9121 ccttggcctt tgacgattcc gcgaccatcg aaagcnnnnn nnnnnnngcg tttgccggtt
     9181 ctttatggag cgaaaatttt caactgaaaa ttcaagtatc atttcaaaat aagtttattt
     9241 tttgataaat attcgcaaat attcatataa tatttacaaa attcaaccta attctttgct
     9301 tttttttatt ttttctacag ccgggttcaa tttgaatcaa ccccaatcag gaagtgaatg
     9361 gggcggtggt gtttatgaat tgaagttttt ctttcaatcc tgactgtttt taaattcccc
     9421 ctcttatcca catactgcca ggcatcgctc tcccggccga ccgccatccc tccctacttc
     9481 aacttctccc tgaaaaacgc caccgtccgt tgccatgcca atnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnatc atagcggggt gtggaatcgt tgtgaaaacc gtgattgacc
     9601 ccgggataaa ggtgggcgct gtattctttg tcgtttgcct gcagcgcggc ggcgtaggcg
     9661 ggccagcctt cattgacacg cgtatcaagc cccgcaaagt gcagcagcag cggcgctttg
     9721 atggaaggca cttcctcagc aggggcctgt cccccataaa aaggtactgc ggccatcagg
     9781 tcgggtatcc gcaccgccat catattggag acccagccgc caaagcaaaa accgactacg
     9841 cctactttgc cattgcattc ggggtgggtt ttcaggtact caaatgcagc gataaaatcc
     9901 tgcaacattt cctccctgtc ccttttggcc tgtaattcac ggcctgcatc gtcggtgccg
     9961 ggataaccac ccagaggtgt cagcgcatcc gggccaatgg aaataaagcc ctccagagct
    10021 gcccggcggg ccacgtcttc tatatgcgga ttgaggcccc tgttttcatg cactaccacg
    10081 atgccgggca atttacccgt gttccccttg ggccgggaaa gcagagcctt gattttgcct
    10141 ccgcccttgg gagaatcata ttccacatat tccgagttga gccgcgggtc gttgggcgct
    10201 acctgaagcg ttcctttata gtcgggcatg ataaaaccaa gaagtgcggg cacggtcaaa
    10261 ccgcccacag cgaagacact gagcttttcc ataaaatccc ttcgatccag ccggttgtgg
    10321 gcataatcgt cataaagatc gaacacttcc tgccggatat ctgctttatt gatttttttc
    10381 attggaacaa gatttatctg tacggttgat ggagtcaggt acattaggtg atgcaaatga
    10441 gtactccttc agattgaaat tttgtgcaag atagacnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnngcatc ccactccgct ttggtggcag cagatttctg atattccatc
    10561 aggcataccc acttgcggcc cttcttcacc agcaatgctt ctaaatgcgt ataactttct
    10621 gaaaggagtt tgcccgtggc atcatgagaa gtgaaataga aaatcccggt ttcatacgct
    10681 gtggtttcgc cgcctattct ttgtgaaaag cgaaacgaga catgatctat ttgctttcct
    10741 tctttggtat ccataaaccc ccgcttccag cgggccagtg cagcggtcat gggttctgaa
    10801 cgcttgtttt ttccggcagt agccaccaac acggcatttt tttggcaagt ggctttgtag
    10861 ccttcaaaat cacctttttt taccgatctg gatacctccg cccagtaggt tctgtttatt
    10921 tcagctattc gcaaggagtc ggttgtaccc gcgctggtcc tgcgccgggc agcccgtgtt
    10981 tgtgccggtg tttgcagagc aaacaggaat gacacgatgc aaaaagggaa aatctttttc
    11041 atttccacca ttgatttttg tttaaagatg aaaacactta tcgagaaaag tatggtaaac
    11101 gaagcgacac agccgccacc cggcccgttg gtgaagaggt gaggcgatgt gccaaaaatg
    11161 gcggcaacat caccccttca caaaacccag acttgcaggc taaccgcaga acgcggcact
    11221 gcctcaccgg atggtaaaag tctttttttc ttttcctaca ccttttatcg tcaatgattt
    11281 ccactgggaa ggcaacttgg ttttcaactg tacaatgccc tgctcggaaa tatccaggcc
    11341 gccgatgccc atcagtacgg cctgcaacat gccgcccgcg cccgtagcga aatacggatt
    11401 ggttccgcct gcagtttccg ccagcactcc aaaaggcggc acctcgttgg gtttgtagct
    11461 ttttacaaac atctggtagg ctttttctga attgccgagg cgggcgtgca gggcagccaa
    11521 cacagagtgg cccatcgcgg gaccgtcggg ggccatgcgc ggctcgtagt aagcgaggtc
    11581 tttgcgaaca tcggcctctt tggtcacgat tttgagcgga aaggccagca ggtttacatc
    11641 ggcctgtttg atcgtaacgc cgtcgtaggt ggcattttcg cgggtagtac cgtcggggaa
    11701 tttgagaatg ggaatgtttt gggcgacgtg cagccaatcg ggattgggcg tcagaccgag
    11761 ttcctgcgcg gcggcggcgg cgtattgcag caccgtgacg gccatgccgt tggtgaaggc
    11821 gttgttgtct atgttttcct gccactcgtt ggcgccgatg acgttgttga tgtcgtactt
    11881 gccgggaccg ttgcgctcca ccctgccggc ccagaaatcg gccacttctt tgagcatggg
    11941 atagcctttt tcgcgcagcc attctttgtc tttggtgacc aaataatact gccagaacga
    12001 ccatcccacg cagccggtga tgtggtgttg gaaaggcccg gtgagcgccc atannnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn ntggttgggt caaaataagc ggtgggatga tgaacagttc ggtatcccaa
    12241 aaaacgtggc cgttgtagcc caagcccgac aagcccatcg gcgacaggct cagggccgtg
    12301 ccttcgcggg caaaggagta caggtggtac aacgccgagc gaatggcccg ctgcgattcc
    12361 agatcgcctt ccaccacgat gtcgctttgc cagagcttgt tccactcggc ttcgtggcgg
    12421 cgcaggaggc gttcgcggcc ttccaatttg gtaaaaatag tgaggcgttc ggcctcgttg
    12481 tgcgggtcgt tgtaggtgtg ggaagacacg gtactgccca ccaagctgaa acggtaggtt
    12541 tcgccggctt tcagtttttt gtaaaacttc gccaggtgca tgttgtaatc ccagtcttcg
    12601 tgtatgattt tcggctcctg tccgtgcggc tcctcgaaga taaaactggt ggaagccgcc
    12661 accgtgtagc gcccggaagg acttttgccc accgatgtaa gcagcggaat gaggacgtgc
    12721 gggcggtcaa tttcgctgta ataattgcgc acatcggcta agtgagtggg cgcttcaatg
    12781 acgctcatcg gcgtgatggt tacgtctttt tttgcggtga tttccaccac gctcagggcc
    12841 gtgtaaggca ggtgccgcag ggccatcgtg gagtgccgca cgctgagttg atcgcccacg
    12901 tcaaaagtag tgagcagcgt ggcggtttgc atgttcaaag tctgggcgta gttggcaata
    12961 tcgcggcgac cgatgcggcg gccgtccacg tccaagttca tgttcagatg gttgaacgtt
    13021 ttgaggatgt tgctcacgcg gcctctttgg tagtaatcat aagcgccatt gagcaccaca
    13081 tcctgcacgc gcatgggttc gggcgaggaa accaggccga tcatgccgtt ggccactgtg
    13141 atgccgaaat agcggccggg gtcaatgtcg cgggcctgaa tatgccaggg cgattggctc
    13201 aggtcttgct gagcggagag ttggaccact ccaaagagaa gcgcgagaag gatgcggttt
    13261 ttcatgcggc gattttttgg gcaaatgtag gagaaaagga gcaagaggga gaagggtggg
    13321 gccaaaagtg ccattactcg ctgttttctg cgatctcaat aacacagccg gccggtcatc
    13381 gagccattga gggtttgccc aacttcgtaa tctattggca atctacatcc gcaccaccct
    13441 cccgtagcct tttttgcctc ccgctgtttc tattgcaaag aaatacaccc cgctgctgta
    13501 cgccgctacc gacaattcaa gggcgtgtcc ccgaccttct acggtggtca ttacatggcc
    13561 cgatgcgttc cacaactgta gccggaagga ctcattttca gcgccctgcc attgtatctg
    13621 caccgattcc cgggccggat tggggaatac gcgaatggac gatatttcaa atgccggatt
    13681 gtagatgtcc gtaggccgca aaacgaccac cggaatcgta atttcactga cattcccaca
    13741 ggcatcccgg ccgctgaaca ccacatgaag ggtgtccgga tcgagacagt ccacctgttc
    13801 tgtgtagaaa taagtaatgg aactcgttcc gcaatcatct tccaccatgc cgccgccgct
    13861 gttggccaac cagggcagaa acgcgcccca gaggtcttcg ccgcattgca atacaatcgg
    13921 agtgggtagc tgcgtccaaa cgggtggggt agtatctctc acctcaatca tcgccgaacg
    13981 ggtactcctg tttccacagg catcttcagc cgtgaannnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn aaccattgct caaaccgttg tagttgtaac tcatggcgat ggcagaacaa
    14101 gcatctgtcg ccgctccgcc gccggcctgc tgaagccata cctgaatggt ggcctccgtc
    14161 gcagcgccgc agtacaatat cagattttcc ggggccgccg tccacacggg cgctacggtg
    14221 tccaccaatc gtatccgggc gctcagactg gcggcattac cacaggcatc ctccgcgatg
    14281 aaggtgacgc ttgtttcgct cgtattgccg caaccgggcg tcggctggcc taaaacgtgg
    14341 cgccagctca ccgaagaaca agcgccctcg gcctgtgcgc cgccatgttc cagcagccat
    14401 tgctggatgc ggacgggcaa atccggcgcg tcgatcggag gccggcgcgg tgatggcggg
    14461 cggcaggctg ttgctgacga tgaagtacgc ctgtatctgc gcggtattgc cacactcgtc
    14521 gacagcgatg aaggttacta aggtggagtc tgtgcaggcg cccgacaatt cgtacgcgta
    14581 ggtaacggca accgttccgc aggcatccga tacctctccg ccgccgttgt ccaacagcca
    14641 gtcggccaat ctggtcggca ggtcctcgct gcacgctatc tcaacggatt gaggcagtat
    14701 ttcccaaacg ggcggaatgg tatccatagc agacagggtg gcaatgctcg aactactgtt
    14761 gccccatcgg tcggtggcgg taaaagtcac agtagccgtg ccgctgaatc cattgcaacc
    14821 gccgctcagc gcagtgtaat cattgctgag ggtaatgggc atgctgtggt cgctcactaa
    14881 agccccgccg gcgttgttca gccattgttg aatgagcgca tccacgtcca tgttgcgatg
    14941 gcagttgaaa acgagatcgg caggcggcgt ttcccatacc ggcgccaggg catcggggac
    15001 aaaatcaacg cccagcgtat ggaaaaccaa gttggtaatg atcggcgcat tgaaatcgaa
    15061 ataaatggcc gcctgattgc ggatgatggc gccgatgata ttgccggact tttgcgatac
    15121 ccggaaccgg atgtacccat gcgatcccgg ctcatcggtg atcgtatcgg tgagatggat
    15181 gtgataaaaa gcaaattcca gcgccccggt gctgtccaca ttcaattgat acgggtggct
    15241 gaaaccggtg acctccaccg tagccggatc gagaaacgcc gacagggtat cgcgcacaaa
    15301 gacgtgaaaa gccgtgtcgt tgcccacatt ctggaaacga accagatatt cgatgtccgt
    15361 gccgggttcg atgaggtgat cgggtccata gcccaccgga aaggcggttt tgtcgttggg
    15421 gtcgtagctg cccgtgatgg ggaaacacac tttgtcctca aaggggcagt tggaatcatc
    15481 cggaaactga agggcatagc tcgtaacggc ctgtcccggc agccctggcc cgcatccttc
    15541 gacaaatacg gaaggcaaca cgctgcaagg atgccccggc gcctgatcgg tttccaatcg
    15601 ccaggcattg ttgccgcccg gcctgtggaa gactaagctg tcgcccgccg cgagccggta
    15661 tcgcgctgct tcaacgagca ctccgttttg gtagatgcgg tattccaaag agtcggcggg
    15721 ggcttggtgg ccggtgttgc ggaagaggag gtgggcttgg tcgttctcgc atctgccgga
    15781 aacatccatc gttgcgccgg gccatgtgcc ggacggagca ttgcacaagg tatctgggta
    15841 aatatgcgct ctgaaacact ggactgcgtt tagcggagcg tcgcaatcaa tacttacata
    15901 tactgaaaat gtcttgcatt gaccaacgtc taaattcccg atatccgaga cgtagtgtcc
    15961 tccgcccaaa tggacaatgg gcacactggc agaatccagc acaatccatt catccagcca
    16021 aaactccacc cttgcatttc cttcaggcgc ggttcctttg ttgcaatatc cgatggcata
    16081 tttattggaa aaacaccttc tgtagaaagg gttttgaata ccgacattga cctgaagttc
    16141 cgggcaaaac gtgaaggatt gaacagggat ggaaagctct agggtgtcca gataagtaac
    16201 ctgtatattg taaatgccgg ggcataaaga ccactgattg ttgggtagca gtacttccaa
    16261 ctgatattga ccggtgtcca ttacaaaatg gaattcaccc tgggcatttg tcctgccata
    16321 gtaagtactt gcccctacca ttcgcacatc ccaattggcc agactctctt cggactcctc
    16381 tggaatacaa ttgctattcg tatcgtgaac aagccgtcct tttacaaccg gataagcatc
    16441 ccggatgtaa aaagtcggag tgctcatttc tgtcaggccg ggcaggtcgg ggtgcgagat
    16501 gtacacgatg aaagaatcct gggggttaaa ggtttctaca aatatttggt tcgtttccgt
    16561 ctcccccata aagtagggca aagaccctct gttgtaccac tggaaacgcc cgcccaccac
    16621 atcttgatca atgcccagat tgatgtagta gccgccggaa ggaacgggaa tgagtttggg
    16681 gctgggtact ggctgctgga acatatattg atactcacta gcatagggta ggtttaataa
    16741 caggtcgtcc aaagacaacc tgttagaacc tatagaaaag gcatatccca gtctgatgga
    16801 tggatcaata aatgcgcatg aatcgaaaag gttggaccca aggcttaatt ctcgtaaaag
    16861 tgggttttcc gtaaaagtag gaattagacc tgttagctta ttttcaccaa gcgccaatct
    16921 ttgcaattgt ggtttgtttg aaaagtcagg aagaatgccg gtaaaatcat ttccataaag
    16981 gtataagctt gtcaaattat tttggaactc nnnnnnnnnn nnnnnnagaa ggaatagggc
    17041 cagaaaagtt gttgttttga gcataaatat gttcaactcc gggacagttt tcgatctttg
    17101 ggaatcgccc aatgaggtga ttgtcggata aatacaaaga tttaaggttc ggcaatccat
    17161 caaaattagg taattgaccc aaaattcgag agcttgatat ttccaataca tccaaatatg
    17221 gaagttggta attgggtatt tctccggaaa gttgtgtgga actaaaaaag atttggcgta
    17281 ataaaggaca atgctggaac aaaggcagat taccggagac ttcactgtcg gacacttcta
    17341 tggtttttaa ttggggcaga tgagaaaaat ccggcagatt nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnatt ctcaattcgc tgacttcggg gatgtgagaa aaatctggta
    17461 aattccctga acctgtgaca attagctttc tcaaattggg tatgaacgaa aaatcgggaa
    17521 tactgccaat cacccttcct atctccaacg agttcaaaag ggggatattt gaaaagtcag
    17581 gaatatcacc tgaaaaatta ccaactaaac tcatagtgct caaaaaaggg aggtaagcaa
    17641 agtctggaat tgttcccgta gaatttcctc ccccaatttt cagacttctg aggtttggca
    17701 aattagtaaa gtccgggatg gtatctgtca agttgttact tgaaatgaaa aaactctgaa
    17761 gcgcaggaag gttggaaaaa ttcggaattt gccccgacac ttgggtgaat tctaaattaa
    17821 tctcccggag gttcggaatg tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnccatat aacttaatcg tatgaatctg tggaatctga gagaaattgg
    17941 ggagggatcc gccaagttca tagttcgcgc ttagaatcag ggatgaaaga gcaggcagat
    18001 tgatatcaaa aatttcacct tgaagattgt tcttaaaaag atcaagacct gttacacaac
    18061 cctgagcatt taatgttgtg ccataaaacg aatctaaagg ctcgctcaaa tcccaggtat
    18121 ttgtccattc agggccggcc caggcattgt acatctgcac cagctccaac gaatccgccg
    18181 tgcggtcaca ttgagcatga acggtatgaa acgtagaaag aacggcgccc agaaggagca
    18241 acgggtaaag cgactttttc atggcttgtg attt
//
LOCUS       DAORLF010000035        18015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1662_length_18015_cov_1.668263, whole genome shotgun sequence.
ACCESSION   DAORLF010000035 DAORLF010000000
VERSION     DAORLF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18015
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_18015_cov_1.668263"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1315)
                     /locus_tag="PK198_05060"
     CDS             complement(74..1315)
                     /locus_tag="PK198_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRF38134.1"
                     /translation="MDIFTIAAILIVLAAAFGYINVRFLKLPTTIGLMLISILFSTAM
                     LLVGRISPEILAVEAEFVRSIDFTKVLMEGMLGFLLFAGALHVDFSQLKAQRGPIVMF
                     ATLGVVASTFLVGGLMYGVFTLLGLHIPFIFCLLFGALISPTDPIAVLGILKKAGVPK
                     KLETKIVGESLFNDGIGVVVFLSLFQLARAGMQEIHWDEIGLLFLEEVGGGIALGLAL
                     GFAAYHLMRTIDNYEVEILITLAIVMGGSLLAATLHFSAPLAMVVAGLMVGHERFRTS
                     SMSETTERYVDKFWELIDTLLNALLFVIIGLEIIVINFEDNYVLAGLIAIPVVLLARY
                     ASLWLPVRLFQKRLEFVPRTDLIMTWGGLRGGISIALALTLSAGMPRDLMLTVTYIVV
                     VFSIVVQGLSVGKVVEKVVKT"
     gene            complement(1352..1882)
                     /locus_tag="PK198_05065"
     CDS             complement(1352..1882)
                     /locus_tag="PK198_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF38135.1"
                     /translation="MHPSLSRRLRAVNDMIEAMYEQLKAHSDEHLNRRPGPQQWSVLQ
                     VMHHLIQAETLSERYVRKKLSFNPELKPAGIAGAFRLFLVRINFVLPFKYKAPKGVGD
                     DVLPETSTLEETIQQWRKQRESLSELLENLPEGYHAKALYKHPLAGKLSLVQMLHFFE
                     IHIQRHQGQIERTLGN"
     gene            complement(1885..4002)
                     /locus_tag="PK198_05070"
     CDS             complement(1885..4002)
                     /locus_tag="PK198_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF38136.1"
                     /translation="MRFLLFIALTLSYTAVGAQTGKNKITVKYPTTRADNQTDNYHGT
                     LVSDPYRWLEDDNSPETSAWVKEQNLVTSTYLSQIPYRDALKKRITELINYPRFSAPI
                     KAGEYYLFYKNDGLQNQSVIYIQKGLKGQAEVFIDPNTLSSDGTVSVSLLGPSDDNRY
                     MAYAQSEAGSDWQQIRVMEIATRKQLPDVLRWVKFSGASWHKDGFFYSRYPQPAKGAE
                     LSGNNQFHSIYYHQIGEDQSKDKLIYEDKANPDYYHYGGVTEDHKYFIMYAAPGTDGY
                     ATYYKDLQNDGPMRPLFEGYANKSSVVEHINGRFLILTDIGAPKYRLVSVDPAKPEQK
                     NWKEILPETDNLLENVSTGGGKMFASYLENATGRIYQMNYDGSDRKEIKLPGLGSAGG
                     LGGKAGDKELFYAFTSFVYPNTIFKYDVVSGVSEPFFESPLQFNPEDYVEQQVFYTSK
                     DGTKVPMFLVYKKGLKMNGKNPCYLYGYGGFNVNLTPSFSASRLALLENGGIYAMPNL
                     RGGAEYGEAWHQAGMLFKKQNVFDDFIAAAEYLIREKYTSQGKLGIGGGSNGGLLVGA
                     CMTQRPDLFAVALPAVGVMDMLRYHLFTVGKGWIPEYGSSEDPAHFKNLYAYSPLHNL
                     KPGVKYPATMVTTADHDDRVVPAHSFKFAAQLQKSHKGSNPVLIRIEERAGHGAGKPV
                     SKVIEEAADVWAFFFFNTKTKIK"
     gene            4206..>4544
                     /locus_tag="PK198_05075"
     CDS             4206..>4544
                     /locus_tag="PK198_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRF38137.1"
                     /translation="MDHISSTAVTSPKIRLHFLDALRAFAILMMLQGHFVDTLLAPEY
                     RDLNSPIFNAWTFMRGLTAPVFFTVTGIVFVFLLLKENKPLKEHVRVRKGIRRAFFLL
                     ALGYLLKWNVY"
     assembly_gap    4545..4554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4555..>4857
                     /locus_tag="PK198_05080"
     CDS             <4555..>4857
                     /locus_tag="PK198_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRF38138.1"
                     /translation="VLSLNFAPYHIAVDVLHVIGLALLALIGIYAFHRVTGASYPFVS
                     GLLGLSVFLVFPVIEQADWSNLPIVFQNYMTMENGSTFTILPWLGYTLIGGVIGSV"
     assembly_gap    4858..4976
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4977..5492
                     /locus_tag="PK198_05085"
     CDS             <4977..5492
                     /locus_tag="PK198_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38139.1"
                     /translation="LSQMWLSKQYLFWRFGHVLVVLSLFVFFEQILKKGFHPLFLKIG
                     SETLTVYGGHYVILYGTWFGIGLSQLWKRSLTPEQTLLGVALFELFFILLIAHIEKVR
                     QVWYEALPAMLVRAFRKIRVMVLRRSRRILLEYRREQPAIVQAIQVMAFQILHRAFHL
                     IPANTLRRYTK"
     gene            5596..6144
                     /locus_tag="PK198_05090"
     CDS             5596..6144
                     /locus_tag="PK198_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38140.1"
                     /translation="MKNRITPIAVLAIMAFMSACSPRYTPFTQRLYDEHRWSEDELKR
                     IQFYLSNDLVLRRELGGTNVQIVSGEIKVRDGRKVEEIFIRQGTPGVFLFSPKENRFA
                     ISFESGATNNRYLMFGPSARLNDRFALLGAEWDRQGGTVTYDGQQYQVDASGALAGLL
                     VNLKRARQVEVNSRVVGGRKVR"
     gene            6211..>6440
                     /locus_tag="PK198_05095"
     CDS             6211..>6440
                     /locus_tag="PK198_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38141.1"
                     /translation="MRTAFFFAIIFSAIMAIHQSCAPTSPGFSRFEEYPYYPEDDLGL
                     RYTPSKSVFKLWAPTANAARISFYEQDMGGEP"
     assembly_gap    6441..6460
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6461..>7648
                     /gene="pulA"
                     /locus_tag="PK198_05100"
     CDS             <6461..>7648
                     /gene="pulA"
                     /locus_tag="PK198_05100"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRF38142.1"
                     /translation="MKKGLDGVWEATIKKTDTKGWYYTYQVRIGETWMGETPDPYAKA
                     VGTNGLRAQILDMRDTNPDGWENDRRPEQASYNDIVLYELHVRDLSIHPNSGIQNKGK
                     YLGLAETGAKSSEGLATGLDHIKDLGVTHVHLLPVYDYMSVDESKLQENKFNWGYDPQ
                     NYNVPEGSYATNPADGAVRIREFKTMVKALHDAGIRVVMDVVYNHTGATELSVFNQLV
                     PGYYYRQTADGGFSNASGCGNETASDRLMMRKLIVESVKYWASEYHIDGFRFDLMGIH
                     DIETMNLVSAELRKLSPSIFVYGEGWTAGDSPLAPEKRAIKHNVLQLEHVAAFSDDVR
                     DAIKGHVFHPEQKGFVSGLAGLEESLKFGIVASTQHPQVDYEKINYSKAPWAKVPAQT
                     ITYA"
     assembly_gap    7649..7828
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7829..>8135
                     /locus_tag="PK198_05105"
     CDS             <7829..>8135
                     /locus_tag="PK198_05105"
                     /inference="COORDINATES: protein motif:HMM:NF023280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HRF38143.1"
                     /translation="ISDLHSINRIDWSRKARYSNVFNYYKGLIALRKSHPAFKLPTQD
                     LIQKHLQFLDTNESNLVAYLLKDNAGGDAWKDILVIFNGNSTAKSVSVPQGNWTLAVD
                     "
     assembly_gap    8136..8246
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8247..>8510)
                     /locus_tag="PK198_05110"
     CDS             complement(<8247..>8510)
                     /locus_tag="PK198_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRF38144.1"
                     /translation="ANILRIQLERQDIKYFPSLREYALYFGIDKALLNRLDKPVVIMH
                     PGPINRGVEITSDAADSEHSIILQQVENGVAVRMAALYLLAGKR"
     assembly_gap    8511..8608
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8609..9180)
                     /locus_tag="PK198_05115"
     CDS             complement(<8609..9180)
                     /locus_tag="PK198_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRF38145.1"
                     /translation="MSQANSLSVPHLLGIKDLTKEDITLILDTAVTFKEVINRPVKKV
                     PSLRDLTVANLFFEDSTRTRISFELAEKRLSADVINFSASSSSVKKGETLLDTVNNIL
                     AMKLYMIVMRHPAPGAPHFLARHISARILNAGDGTHEHPTQALLDAYSMREQLGDLEG
                     KKIAIVGDILHSRVALSNIFCLKKLGAEVKV"
     gene            complement(9180..9740)
                     /gene="pyrR"
                     /locus_tag="PK198_05120"
     CDS             complement(9180..9740)
                     /gene="pyrR"
                     /locus_tag="PK198_05120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRF38146.1"
                     /translation="MNQERIILSQERFALTIERLCHQLIEHFDRFEDACIIGIQPRGV
                     LLSDRIHARLMQLLDAPSLLYGKMDITFYRDDYRLRSKPLLPSTTSMDFQVDNKNVLL
                     IDDVLYSGRTVQAALAALNDYGRPRSVELAVLIDRRFNRHLPIQADFTGMAIDALDQA
                     YVQVEWAHTDGEDRVLLFADKKDRDK"
     gene            9985..10245
                     /locus_tag="PK198_05125"
     CDS             9985..10245
                     /locus_tag="PK198_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38147.1"
                     /translation="MKSRIIKRRLVKARSALNQTIQKILDISRIKKTIPYAENPEVKE
                     AGISEELWLLNKLAKQQAQLVRHYETTLTEDKMMPPKNNFLR"
     assembly_gap    10330..10342
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10343..11669)
                     /locus_tag="PK198_05130"
     CDS             complement(<10343..11669)
                     /locus_tag="PK198_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38148.1"
                     /translation="MRNKLQKFGDFAAGLLPHETTYLLSVQHFEDDVKLGILKLMDEN
                     CHKIRQQAPYDTEIDKRKYSNLKSWIEERLASIDVDERFDWLSLMERKIMTDAIEPQE
                     EKTLLKAIRDYEQPDFYFVKFYELVAVYRHFLLIRMRHAQHRQANEFLKKFKAQYEYS
                     KEIFERMHDATEDIVNQYSKNSAQSLHWENWLTEVYYNEALDGLNRYFALVRLTFIYF
                     NYRNFEKLLEKYEYIDRLFSQGIYYSRRLLLNYYGNRLLLHTRFHEYDKAEYYGYLSI
                     REKTADYIFYVNNLAAVLMRQNKYAEALQLMRSAYPEMKTTAGFHNRIGFVALYLKCL
                     NHNDQYRSAENYAETFLSAYRQEVFEYRWHIFFTSYIEALLHQEKYAKILKIIRKHQL
                     AERETQYRKNANYLPAIQWYQWVAMYKESLIQRDELERHLADSLTQTDRL"
     gene            complement(11755..>11985)
                     /locus_tag="PK198_05135"
     CDS             complement(11755..>11985)
                     /locus_tag="PK198_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF38149.1"
                     /translation="ELGRKGITVNAIAPGFIGTEMITSMPEKVIQMMNDKTPLGRIGT
                     PEEVAKAYLFLASEDASYVSGAVLSVDGGVSI"
     assembly_gap    11986..12019
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12020..12498)
                     /locus_tag="PK198_05140"
     CDS             complement(<12020..12498)
                     /locus_tag="PK198_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF38150.1"
                     /translation="MKRLEQRVAIVTGGASGIGKAIAALFAAEGASVAIWDWNEERGE
                     AAAAELNGKFYQVNTADFAMVEAAAQKTNEDFGRIDILINNAGILRDATLMKITAEQW
                     QQVIDVNLTGVFNCTKAVAPFMVAKGYGRIVNASSVVGLYGNFGQTNYVATKAGVIG"
     gene            12740..>13636
                     /locus_tag="PK198_05145"
     CDS             12740..>13636
                     /locus_tag="PK198_05145"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF38151.1"
                     /translation="MKQAYIVSAVRTPIGSFGGLLAEIPATQLGAIAIKGAIEKAGVP
                     AHLVEEVYFGNVVSANLGQAPARQAALGAGLSPSVQCTTVNKVCASGTKSIMFAAQSI
                     MLGLADIVVAGGMENMSMIPFYIPKARFGYKYGNGEIVDGLSRDGLLDVYDQQAMGVF
                     ADRTAEKYEITRQDQDAFAIRSYKRSAESTQAGKFTDEIIPVAIPQRKGEPLLITEDE
                     EYKKVNFDKIADLRPAFTKEGSVTAANASTINDGAAALVLVSEAKLKELGLKPIARVV
                     SFADAEQEPEWFTTTPTLAAPKA"
     assembly_gap    13637..13664
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13665..13932
                     /locus_tag="PK198_05150"
     CDS             <13665..13932
                     /locus_tag="PK198_05150"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661134.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRF38152.1"
                     /translation="LTMNDIDFFEINEAFSVVTLAFNKVLDVDINKVNVHGGAVSLGH
                     PLGASGARIVTTLNGVLHQNKGKYGMAAICNGGGGASAIIIEAL"
     gene            complement(14005..>14533)
                     /locus_tag="PK198_05155"
     CDS             complement(14005..>14533)
                     /locus_tag="PK198_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRF38153.1"
                     /translation="PQTANKQDYIGAFAVTAGIGIETWVARFEKDHDDYSAILLKALA
                     DRLAEALAERLHERVRREFWGYVANESLDNQSLIEEKYQGIRPAPGYPACPEHSEKQT
                     LWNLLDVEQRTGIRLTESFAMYPAASVSGWYFSHPESKYFGISEIAPDQAADYAARKG
                     WTEEEAARGLSSVLG"
     assembly_gap    14534..14671
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14672..>14998)
                     /locus_tag="PK198_05160"
     CDS             complement(<14672..>14998)
                     /locus_tag="PK198_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRF38154.1"
                     /translation="RKAAKNFLSIEAARANRFRPDWEQYTPPVPAQPGVQHVEAWPLE
                     ELTEYIDWTPFFATWELAGKFPAILDDEIVGTEARRLYHDAQSMLRRIIDEKWLRASA
                     VFGLFPA"
     assembly_gap    14999..15225
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15226..>16427)
                     /locus_tag="PK198_05165"
     CDS             complement(<15226..>16427)
                     /locus_tag="PK198_05165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRF38155.1"
                     /translation="NLIASEPDIARIPIMIDSSKWEILEAGLKCVQGKCVVNSISLKE
                     GEADFIEKAKKVKRYGAAVIVMAFDEDGQADSYERRIEICGRAYKILIEKVGFNKHDI
                     IFDPNIFAVATGIEEHNQYAINYIEATRLIKQHCPGAKISGGVSNISFSFRGNDRVRE
                     AMHSAFLYHAIQAGMDMGIVNAGMIEVYEEIPKDLLQLVEDVLLNRNPKATEALTDYA
                     ERIKGGGRVLQKDTAWRSESLESRLSHALVKGITDHIDDDVEEARLSYSSPLAVIEGP
                     LMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLQPFIEADKQASGDQSAKGRILM
                     ATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVPAAAILDAARQHRVDIIGLSGLITP
                     SLDEMVHV"
     assembly_gap    16428..16554
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16555..16742)
                     /locus_tag="PK198_05170"
     CDS             complement(<16555..16742)
                     /locus_tag="PK198_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38156.1"
                     /translation="MNKKDNIIEVDFELAKGQELKAKGYKLKLSGLEPLIATNESNFI
                     NVGERCNVTGSKAFLNLI"
     gene            complement(16739..17089)
                     /locus_tag="PK198_05175"
     CDS             complement(16739..17089)
                     /locus_tag="PK198_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF38157.1"
                     /translation="MKIWQQGMQIVKATYRLTSLLPKEEKYGIVSQMNRAAVSIPSNI
                     AEGCSRNGEIDFKRFLEIAQGSAFELETQLIIIEEIGLMKSDSENFISLITLIHEEQR
                     MINSLITKIKTEHN"
     gene            complement(17146..>17699)
                     /locus_tag="PK198_05180"
     CDS             complement(17146..>17699)
                     /locus_tag="PK198_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRF38158.1"
                     /translation="ELVVAFKQQVQALVEGGVDLLLIETITDTLNAKACLYAIQEYFE
                     ETGKELPVMISGTITDASGRILSGQTTEAFLISVSHVPLLSIGFNCAMGAEALMQYVE
                     ILSDNADCLVSAYANAGLPNAMGQYDQTAEDMAQLMKPYFEKGLINIVGGCCGTTPDH
                     IKAIAQVAKNYAPRITKPLAVGL"
     assembly_gap    17700..17737
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17738..>18015)
                     /locus_tag="PK198_05185"
     CDS             complement(<17738..>18015)
                     /locus_tag="PK198_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRF38159.1"
                     /translation="DLLSITRPDIIKAIHAAYFDADVDIIETNTFSGTTVSQADYGLE
                     HLVYDLNYYSAKIAREVADEYTAKNPAKPRFVAGSIGPTTKLSSMSPD"
BASE COUNT         4035 a         4230 c         4253 g         4354 t
ORIGIN      
        1 acaatcgtta aattaaaaca attctcgcgc catgcatacc atcaacatta tcacattcat
       61 ggttgccttt agactacgtc ttcaccacct tctccaccac ctttcccaca ctaagcccct
      121 gaaccacaat ggaaaatacc actacgatgt aagtaacggt gagcatgaga tcgcggggca
      181 taccggcact gagcgtcagg gctaaggcaa tggaaatacc gccgcgaaga ccgccccaag
      241 tcatgatgag atcggtacga ggtacgaatt cgaggcgttt ttgaaacaat cgcaccggca
      301 gccacagcga ggcataacgg gccagcagca ccaccggaat ggcgatgaga ccggccaaaa
      361 cgtagttgtc ctcaaaattg atgacgatga tttccaaacc tatgatgaca aaaagcaggg
      421 cgttgagcag cgtgtctatc aattcccaaa acttgtccac atagcgctcg gtggtttcag
      481 acatggaaga ggtgcgaaaa cgctcgtgcc ccaccatcag gcccgccacc accatagcca
      541 gcggggcgga aaagtgcagg gtggctgcca acaaagagcc tcccatgaca atggctaagg
      601 tgatgagaat ttccacctca taattatcta tggtgcgcat gaggtggtaa gctgcgaaac
      661 ccagcgccaa gcccagcgcg ataccgccgc ccacttcttc caaaaacaac agcccgattt
      721 catcccagtg gatttcctgc atacctgccc gcgctaattg aaacaaactg agaaacacca
      781 ccacgcctat gccgtcgttg aaaaggcttt cacctactat cttggtttcc aattttttag
      841 ggacgccggc tttcttcaaa atacccaaaa cggctatggg gtcggtaggc gagatgagcg
      901 caccgaacaa caggcagaaa atgaagggaa tgtgcagccc cagcaaggtg aatacgccgt
      961 acatcagtcc gccgaccaaa aaagtggagg ctactacgcc caaagtggca aacatgacga
     1021 tgggacctct ttgagctttc agttgactga aatcaacatg cagggcgccc gcaaacaata
     1081 gaaagccgag catgccctcc atgagtactt tggtaaaatc tatgctcctc acaaactcgg
     1141 cttccacggc taaaatttca ggcgaaatgc gccccaccag caacatagcc gtcgaaaaca
     1201 ggatggaaat gagcatcagc ccgatggtgg tgggcagctt gagaaaacgc acattgatgt
     1261 agccgaaagc cgcggccagc acaatcagga tggcggctat ggtgaagatg tccattgata
     1321 cgatggtttg ttcaaactac cccgaacaac ctcagttgcc gagtgttcgc tcaatttgcc
     1381 cctgatgtct ttggatgtgt atttcaaaaa aatggagcat ttgcaccaac gacaactttc
     1441 cggccagcgg gtgtttgtac agggctttgg catggtagcc ttccggcaga ttttccagca
     1501 gctcgctcaa actctcccgc tgcttgcgcc attgctgtat ggtttcttct aaagtggaag
     1561 tctccgggag tacgtcgtcg cctactcctt tgggggcttt gtatttgaag ggcaacacaa
     1621 aattgatgcg caccaaaaac agccggaatg ctccggctat cccggcaggt ttcagttccg
     1681 gattgaaact cagctttttg cgaacatagc gctccgaaag cgtctcggcc tgtatgaggt
     1741 ggtgcatgac ttgcaaaaca gaccattgct gcggccccgg cctgcgattg aggtgctcgt
     1801 cactgtgcgc tttgagctgc tcgtacattg cttcaatcat gtcattgact gcccgtagcc
     1861 gccgtgatag ggatgggtgc atgtttattt tattttggtt ttggtattga aaaagaaaaa
     1921 ggcccacaca tccgctgcct cctcaataac tttggaaaca ggcttgcccg cgccatgccc
     1981 ggcccgctcc tcaatgcgaa tgaggacagg attggagcct ttgtggcttt tttgcaattg
     2041 tgcggcaaac ttgaacgaat gcgccggcac tacccggtcg tcgtggtcgg ccgtggtgac
     2101 catcgtggca gggtatttca cgcccggctt cagattgtgc agcggcgagt aggcatacag
     2161 gtttttgaaa tgcgcagggt cttcgctgga gccgtactcc ggtatccagc ctttgccgac
     2221 agtgaacaag tgataccgca gcatatccat cacgcctaca gccggcagcg ctaccgcaaa
     2281 cagatcgggc cgttgggtca tgcaggcgcc tacgagcaag ccgccgttgg agcctccgcc
     2341 gatgccaagt ttgccttggg aggtatattt ttcgcgaatg agatactccg ccgctgcgat
     2401 aaaatcatca aaaacattct gctttttgaa caacatgccc gcctgatgcc aagcctctcc
     2461 gtattcggcg ccgccgcgca aattgggcat ggcataaata ccgccgtttt cgagcaaggc
     2521 cagccgggaa gcactgaatg agggggtcag attgacgttg aagccgccat aaccatagag
     2581 gtaacagggg tttttgccgt tcattttcag tcctttttta tagaccaaaa acatgggtac
     2641 tttggtgccg tctttactgg tataaaaaac ctgttgctct acataatctt caggattgaa
     2701 ctggagcggc gactcaaaaa acggctcgga aacgccgctg accacatcat acttaaatat
     2761 ggtgttggga taaacaaaag aggtaaaggc ataaaacaac tccttgtcgc cggcctttcc
     2821 gcccagtccg ccggcactgc ccaagcccgg cagtttgatt tccttgcggt cgctgccgtc
     2881 gtagttcatc tgatagatgc ggccggtggc attttcaagg tacgatgcaa acattttgcc
     2941 gccgccggta ctcacgtttt ccagcagatt gtctgtttcc ggcaggattt ccttccaatt
     3001 tttttgctca ggcttggcgg ggtccaccga tacgaggcgg tacttgggtg cgccaatgtc
     3061 ggtgaggatt agaaaacgac cattgatatg ttccaccaca ctgcttttgt tggcatatcc
     3121 ttcaaaaagc gggcgcatgg ggccgtcgtt ttgcaagtct ttgtagtatg tggcgtatcc
     3181 atcggtaccg ggtgcagcat acatgatgaa gtacttgtgg tcttctgtaa cgcctccgta
     3241 gtggtagtaa tcggggtttg ccttgtcttc gtaaatgagt ttgtccttgc tttggtcttc
     3301 gcctatttgg tggtaatata tggagtgaaa ctgattgttg ccgctcagtt cggcgccttt
     3361 ggccggttgc gggtagcggc tgtaaaagaa gccgtctttg tgccaggagg cgcccgaaaa
     3421 cttgacccat cggagcacat cgggcagttg tttgcgggtg gcgatttcca tcacgcgaat
     3481 ttgctgccag tcggaaccag cttctgattg ggcgtaagcc atgtaccggt tgtcgtcgga
     3541 agggccgagt aaacttacgg aaaccgtgcc gtcggaagat agggtattag ggtcaataaa
     3601 gacctccgcc tgccctttca gtcctttttg tatataaata accgactgat tttgaaggcc
     3661 gtcgttcttg taaaacaggt aatactcgcc cgcttttatc ggggccgaaa agcgagggta
     3721 attgatgagt tcggtgatgc gttttttgag cgcatcgcga tagggaattt gagacagata
     3781 agtcgaagtg accaaatttt gctccttcac ccaggcgctc gtttccgggg aattgtcgtc
     3841 ttcgagccag cggtaggggt cggacacgag cgttccgtgg taattatccg tttggttgtc
     3901 tgctctggta gttgggtatt tcacggttat tttgtttttg ccggtttgag cgccaacagc
     3961 cgtatagctg agcgtaagcg caataaaaag aaggaatctc atagaaagtt tgatttaaat
     4021 tcggtcaaaa gtaatctatt ttgggaatcc aagtgttaaa gttgcattgg attcacagca
     4081 aaggcgtaat tttgcagacg aaaacggcag aagaaaattt tgtgacgaaa cgcccggcga
     4141 tccgttcaaa ataacactct tgcagcgcgt gtggccgctt ggttcatctt cttaatcgct
     4201 ctcatttgga tcacatatct tctacggcag ttacttcccc gaaaatcagg ttgcattttc
     4261 tggatgcgct gcgtgctttt gccattctga tgatgcttca ggggcatttt gtggataccc
     4321 tgcttgcccc ggaataccgt gacctcaaca gcccgatatt caatgcttgg acatttatgc
     4381 gcggcctcac ggcgccggtg ttcttcacag tcacaggtat tgtgtttgtc tttttattat
     4441 tgaaagaaaa taagccactc aaggagcatg tgcgtgttcg caagggcata cgcagagcct
     4501 tttttctgct ggcgctgggc tatttgctca aatggaatgt ttacnnnnnn nnnngcgtgc
     4561 tctcgctgaa ttttgcgccc tatcatattg ccgtggacgt attgcatgtc atcggtctgg
     4621 cccttttggc gctgatcggt atatatgcct tccacagagt gacgggggcg tcttacccgt
     4681 ttgtttccgg actgctgggg ctgtctgtct ttctggtttt tcctgtcatt gagcaagcgg
     4741 attggtccaa cctacccatc gtttttcaaa actacatgac gatggaaaac ggatctactt
     4801 ttacgatcct cccctggctg ggctatacgc tcattggcgg cgtcattggt tcagtagnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttat
     4981 ctcagatgtg gttgagtaag cagtatctct tttggcgttt cggccatgta ttggtggtgc
     5041 tttctttgtt tgtgtttttc gagcagattt taaaaaaggg gttccatccc ttgttcctca
     5101 aaatcggctc cgaaacgctc accgtttatg gaggccatta tgtgattttg tacggaacct
     5161 ggtttggtat tggcctgtct cagttgtgga aacgatcact tactccggaa caaacgcttt
     5221 tgggagtagc gctttttgaa ctgtttttta tcctgctgat tgcgcacatc gaaaaagtac
     5281 gccaggtgtg gtacgaggca ttgccggcca tgctggtacg cgcttttcgc aaaatccgcg
     5341 tcatggtgtt gaggcgcagc cggcgcattc tgttggaata ccgccgggag caacccgcta
     5401 tcgttcaagc cattcaggtt atggcattcc agatattgca ccgcgctttt cacctgattc
     5461 ccgcaaatac gcttcgtcgt tacacaaaat aatccttgag aaatctttaa cgattcgggt
     5521 taaagtgtat taaattgctg gacattcagc gaggaaggca gtaatttggc ccctgaaatc
     5581 aaagcactct aaacaatgaa aaatcgcatc acgcccattg ctgtattggc aattatggcc
     5641 ttcatgtctg catgttcgcc ccgctacact cccttcacgc agcgcttgta cgatgagcac
     5701 cgctggtcgg aagatgaact gaagcgcata cagttttacc tctccaacga cctcgttttg
     5761 cgccgcgaat taggtggcac caatgtgcag atagtatccg gcgagatcaa agtgagagat
     5821 ggtcgcaaag tggaagaaat tttcatccgg cagggcacgc cgggcgtctt tttgttcagt
     5881 cccaaagaaa accgttttgc catcagtttt gagtccggag ctaccaacaa tcgctatctg
     5941 atgtttggcc ccagtgccag gctcaatgac cgttttgctc ttttgggcgc cgaatgggat
     6001 cggcaaggcg gtacggtaac ttatgatggt caacaatatc aggtggatgc ctccggcgct
     6061 ctggcgggtt tgttggtcaa tctcaagcgc gcccggcaag tggaggtcaa ctctcgcgtt
     6121 gtcggcggtc gaaaggtgcg atgaatatcc gcagcggttc tcatgcggtt tgccggctgc
     6181 ttactatctt tgccctaaaa tcagcacagc atgagaaccg catttttctt tgccatcata
     6241 ttttcagcca tcatggccat ccaccaatcc tgcgcgccta cttctcccgg cttcagccgc
     6301 tttgaagaat atccgtacta tcccgaagac gacctgggac ttcgctatac gccttccaag
     6361 tcggtgttca aactctgggc gccaacggcc aacgcagcac gcatcagttt ttatgagcaa
     6421 gacatgggcg gtgaacccat nnnnnnnnnn nnnnnnnnnn atgaaaaaag gtttggacgg
     6481 cgtttgggaa gcaaccatca aaaagaccga taccaagggc tggtactaca cttatcaggt
     6541 acgaatcgga gagacctgga tgggtgaaac gcccgatcct tacgccaaag cggtgggtac
     6601 caacggcttg cgcgcccaaa tattagacat gcgcgacacc aaccccgatg gctgggaaaa
     6661 cgaccgccgc ccggaacagg catcttacaa cgatattgtg ctgtacgaac tgcatgtgcg
     6721 cgacctgtcc atccacccca attccggcat acaaaataag gggaaatact taggcttggc
     6781 agaaaccggc gccaaaagct ccgaaggtct ggctaccggc ttagaccata tcaaagactt
     6841 gggagtaacc catgttcacc tgctgccggt atatgattat atgtcggttg acgagtctaa
     6901 attacaggaa aacaagttca attggggcta cgaccctcaa aattacaatg ttccggaagg
     6961 cagttatgct accaaccctg ccgacggcgc cgtgcgcatc agagaattca aaacgatggt
     7021 aaaagccctg cacgatgcag gcatccgggt ggtcatggac gtggtgtata accataccgg
     7081 cgctacggag ctgtctgttt ttaaccagtt ggtgccgggc tattattatc gccaaacggc
     7141 cgatggaggg ttctccaacg ccagcggatg cggcaatgaa acggcctccg accgcctgat
     7201 gatgcgcaaa ctcatcgtcg aatccgtcaa atactgggct tccgaatacc acatagacgg
     7261 cttccgtttt gacctcatgg gcatacatga tatagagacc atgaatttgg tcagcgcaga
     7321 actgcgcaaa ttgtcgccct ccatttttgt ttacggcgaa ggctggaccg ccggcgacag
     7381 cccattggcc cccgaaaaac gcgccatcaa acacaatgta ctccaacttg agcatgtagc
     7441 ggccttcagc gacgacgtgc gcgacgccat caaaggccat gtgtttcatc ctgagcaaaa
     7501 gggcttcgtg agcggcctgg ctggactgga agaaagcctc aagttcggca ttgtcgcatc
     7561 tacccaacac ccgcaagtgg attacgaaaa gatcaactat tccaaagcgc cctgggccaa
     7621 agttccggcc caaaccatca cctacgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cagcgatttg cacagcatca accggattga
     7861 ctggtcgcga aaggcccgtt attccaatgt gttcaattat tacaaagggc tgattgcttt
     7921 gcgcaaaagc caccctgctt tcaaacttcc cacacaagac ctcatccaaa agcatttgca
     7981 gtttttggat accaatgaat ccaatctggt cgcatactta ctgaaagaca atgccggagg
     8041 cgatgcctgg aaagatattc tcgtcatttt caacggcaac agcactgcaa aatccgtatc
     8101 cgttccacaa ggcaactgga cgctggcagt agatgnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnccgc ttccccgcca gcaaatacag ggctgccatg
     8281 cgcacggcca ccccgttttc tacctgttgc aggatgatgg agtgttctga gtcggctgca
     8341 tcgctcgtta tttctacccc tcgattgatg gggccggggt gcatgatgac aacaggcttg
     8401 tccaagcggt tgagcagcgc tttgtcaatg ccgaaataaa gggcgtattc ccgcagcgac
     8461 gggaaatatt tgatgtcctg gcgttccaat tgaattctga gaatattcgc nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac cttgacctct gcgcctaact tcttgaggca
     8641 gaatatattg ctgagcgcta ctctggagtg cagaatatcg cctacaatcg caattttttt
     8701 accttccaga tcgccgagct gctcgcgcat ggagtaggca tccaagagag cctgcgtggg
     8761 atgctcgtgc gtgccgtcgc cggcattgag gatccgcgcc gaaatgtgcc ttgccagaaa
     8821 atgaggtgcg ccgggcgccg gatgccgcat cacaatcatg tacaacttca tggcgaggat
     8881 gttgttcacg gtatcgagca gcgtttcgcc ttttttgacg gaagaagacg atgcggaaaa
     8941 gttgatgacg tcggcagaga ggcgtttttc ggccaattcg aaggagatgc gggtgcgggt
     9001 ggaatcctcg aagaagaggt tggcaacggt gaggtcgcgc agggagggta ctttttttac
     9061 ggggcggttg atgacttcct taaaagtgac ggcggtatcc aatatcaagg tgatgtcctc
     9121 ttttgtcaag tctttgatcc ccagcaagtg gggcacgctc aatgaattag cctgcgacat
     9181 tatttatccc tgtctttttt gtcagcaaag agtaataccc ggtcttcccc gtctgtgtgc
     9241 gcccattcca cctgcacata agcctggtcg agggcatcta tggccatgcc cgtaaaatcg
     9301 gcctgaatgg gcagatgccg attgaagcgg cgatctataa gtactgcaag ttccacagag
     9361 cgcgggcgcc cgtagtcgtt caaggctgcc agcgctgcct gcacggtgcg gcccgaatac
     9421 aggacgtcgt ctatgagcag tacatttttg ttgtccacct gaaaatccat gctggtggtg
     9481 ctgggcaaaa gcggcttgct tcggaggcgg tagtcgtctc tgtaaaaggt gatgtccatt
     9541 ttgccataca gcagggaggg ggcgtctaag agctgcatca ggcgcgcatg aatgcggtcg
     9601 gagaggagta cgccgcgcgg ttgaatgccg atgatgcagg cgtcttcaaa ccggtcgaag
     9661 tgttcgatga gttggtggca caaccgttca atggtgagtg cgaaccgttc ctgactcaat
     9721 atgatacgct cctgattcat tggggcaaag ataagttttt gggaaaggaa agcaagcgtg
     9781 tttttgtttt gaccggcgtg agcggcaaaa gatgcttaaa tggcgtattg tcagcttatt
     9841 gtaatttaac agatgttttt tcattttttt taatgaaaaa ttttacaaaa ttttacgatc
     9901 aaaaattatg ctattgacgt ttatctggag tattttgtgc tcgaattgcc aacctctcta
     9961 accaaaaacc tgtctttttg aaatatgaaa agcagaatca tcaaacgacg gcttgtaaaa
    10021 gcaagaagcg ccttgaatca aaccatccaa aaaatcctcg acatcagccg catcaagaaa
    10081 accatacctt atgcggagaa cccggaagta aaggaggccg ggatcagcga agagttgtgg
    10141 ctgctgaata aattagccaa gcagcaggct caattggtac ggcattacga aacgacactg
    10201 acggaagaca agatgatgcc gcccaaaaat aattttttac gataagctca ggcgctcccg
    10261 cagccggttg atgacgccgg gaatgagcgg gtgtacctcg tccatcaact ccctgagctg
    10321 atggtgcttn nnnnnnnnnn nnccaaacgg tcggtctggg tcaatgaatc agccaaatgg
    10381 cgctccaact catcgcgttg aatgaggctc tccttgtaca tagccaccca ctgataccac
    10441 tgaatggcgg gtaggtaatt ggcattcttc cggtattggg tttcgcgctc ggcaagctgg
    10501 tgtttgcgta tgattttcaa tatcttggcg tatttttcct gatggagcag cgcctcgatg
    10561 taggaagtga aaaagatatg ccagcgatac tcgaacacct cctgccggta tgccgacagg
    10621 aaagtttctg cgtagttttc ggcactgcgg tattggtcgt tgtgattgag acacttgagg
    10681 tagagcgcaa caaacccaat tctgttatgg aaccccgccg tcgttttcat ctccggatac
    10741 gcagatcgca tgagttgaag agcctccgcg tatttgttct ggcgcatcag tacggccgcc
    10801 aggttgttga cgtagaaaat gtagtctgcc gttttttccc ggatggataa gtacccgtaa
    10861 tactctgcct tgtcgtattc gtgaaaacgg gtgtgcagaa gcaggcggtt gccgtaatag
    10921 ttgagcagca gccgccgcga gtaatagatg ccctgagaaa acaggcggtc aatgtattcg
    10981 tacttttcga gcagcttctc gaagttccgg tagttgaaat aaataaaagt gagccgcacc
    11041 aaggcgaagt agcgattgag gccgtccaat gcttcgttgt aatacacctc cgtgagccag
    11101 ttttcccagt gaagggattg ggcagagttt ttggagtatt gattgacgat gtcttccgta
    11161 gcgtcgtgca tacgctcaaa gatctccttg ctgtattcgt actgcgcctt gaattttttc
    11221 aaaaactcat tggcctgcct gtgctgtgcg tgccgcatac ggatgagcag aaaatggcga
    11281 tacacggcta ccaattcgta aaatttgaca aagtagaaat ccggttgctc gtagtcccgg
    11341 atcgccttca ataaagtttt ctcttcctgg ggttctatgg cgtctgtcat gatcttgcgc
    11401 tccatgaggc tgagccagtc aaagcgctca tccacgtcta tggaggccag gcgctcctca
    11461 atccagcttt tgaggttgga gtactttcgc ttgtctattt ctgtatcgta gggggcttgt
    11521 tgcctgattt tgtggcaatt ttcgtccatc agtttcaaaa tgccgagctt cacatcgtct
    11581 tcaaaatgtt ggaccgaaag caggtaggtc gtctcgtgtg gcaacagacc ggcggcaaaa
    11641 tctccgaact tttgaagctt gtttctcaca aatgatctga ttttagtagt acgaatatga
    11701 gccatcccga attttgcaaa acgcgggatg actcatgttt ttgaaacgag gtgcctaaat
    11761 gctcacaccg ccatccacgc tcaatacggc gcccgaaaca tacgaagcat cttcggaagc
    11821 taggaaaagg taggctttgg ccacttcttc cggcgtgccg atgcggccca gcggcgtttt
    11881 gtcgttcatc atctgaatca ctttttcggg catggaggtg atcatttccg tgccgatgaa
    11941 gccgggagca atggcattca ccgtgatgcc tttccggccc agttcnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnna taccgataac gccggctttg gtagccacat agttggtctg
    12061 gccgaaattg ccatacaaac ccactaccga cgaggcattg acgatgcgcc cgtaaccctt
    12121 ggccaccata aacggcgcca ctgctttggt gcaattgaaa acgccggtga ggttcacgtc
    12181 tatgacctgc tgccactgtt cggcggtgat tttcatcaaa gtggcatcgc ggagtatgcc
    12241 ggcattgttg atgagaatgt ctatccggcc aaaatcctca ttggttttct gggcggctgc
    12301 ttccaccatg gcaaaatcgg cggtattgac ttgataaaac ttgccgttga gttcggcggc
    12361 agcagcctcg ccgcgctctt cgttccaatc ccagatggct acgcttgcgc cttcagcggc
    12421 aaacagagcg gcaatggcct ttccgatacc ggaagcgcct cccgtgacga tagctacgcg
    12481 ttgttctaat ctcttcatgt tgcttttttt ttcccaaaat tagagcctct gtctatttgc
    12541 agggtaactc cgatactcaa aacagggccg accaaagata gaaactatat tttattttgg
    12601 tgtaaataac tggttattaa atttttgaat tcaaaataaa acagcattga cccgcccgtt
    12661 gctattgcgc aataattttg atcggcgtac ctttacgcaa cggttttctt tatttctcat
    12721 ctcaaaacaa ataattccaa tgaagcaggc ttacatcgta tcggcagttc gtactcccat
    12781 cggcagtttt ggcggccttt tggcagaaat tccggccacg caattaggcg ctattgccat
    12841 caaaggcgct attgaaaaag caggcgttcc ggcgcatttg gtagaggagg tatatttcgg
    12901 caacgtcgtt tcggccaatc tggggcaggc tccggcaaga caggctgcgc tgggcgccgg
    12961 tttgtctccc tctgtgcaat gtaccactgt caataaagtc tgcgcttcgg gcaccaaatc
    13021 catcatgttt gctgctcaga gcatcatgtt gggcttggcg gatatagttg tagcgggcgg
    13081 tatggaaaac atgagcatga ttccttttta catccccaag gctcgttttg gttataaata
    13141 tggcaatggt gaaatcgttg acggcttgtc cagagatggc ttgttggacg tatatgatca
    13201 acaggcgatg ggcgtttttg ccgaccgcac cgccgagaaa tatgaaatca cccggcaaga
    13261 tcaggatgct tttgccattc gttcttacaa gcgctcggcc gaaagtacgc aggcaggaaa
    13321 gtttacggat gaaatcattc cggtggccat tcctcagcgc aaaggcgaac ccttgctcat
    13381 caccgaagat gaagagtaca aaaaggtgaa ttttgataaa atcgccgatc tgcgccctgc
    13441 ttttaccaaa gaaggcagcg taacggcggc caatgcttcc accatcaatg acggcgcggc
    13501 tgccttggta ttggtgagcg aggctaaatt gaaagaactc ggtttgaagc ccatcgcgcg
    13561 ggtggtatcg tttgccgatg cagagcagga gccggaatgg tttacaacga cgcccacttt
    13621 ggcggcgccc aaagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnncctcac catgaacgat
    13681 attgactttt ttgagatcaa tgaagcgttc tcggtcgtta ctctggcctt taacaaggtc
    13741 ttggacgtgg acatcaacaa ggtgaacgta cacggcggcg cggtttcttt ggggcatccg
    13801 ctgggcgcat ccggtgcgcg catcgtcaca acgctcaatg gcgtgttgca tcaaaataaa
    13861 ggcaaatacg gcatggcggc catctgcaac ggcggcggcg gcgcgtcggc catcatcatt
    13921 gaggcgctgt aagtaattac gagaaaatat tgccggcggc ggagacagga tgatttccgt
    13981 cgccggtttc ttttggtttt acctttaccc caatacactg ctcaaccctc gtgctgcctc
    14041 ctcttccgtc cagccttttc gggcggcgta gtcggcggcc tgatcgggcg ctatttcgct
    14101 gatgccgaag tatttggatt caggatgaga gaaataccag ccgctcaccg atgccgccgg
    14161 atacatggca aagctctctg tgagccgaat gccggtgcgc tgctctacat ccaataaatt
    14221 ccagagcgtt tgtttctcgc tgtgttcggg gcaggcagga taacccggcg ccgggcgaat
    14281 accctgatac ttttcttcta tgagcgactg attgtcaagc gattcattgg caacgtagcc
    14341 ccagaactct cggcgtacac gctcatgcag gcgctcggcc aaggcttccg ccaaacgatc
    14401 tgccaaagcc ttgagcaaaa tagcgctgta atcgtcatga tctttttcaa aacgcgccac
    14461 ccaggtttca attccaatgc ccgccgttac ggcaaaagcg ccgatgtagt cttgtttgtt
    14521 cgccgtttgc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggccgggaa cagcccgaac accgccgatg
    14701 cgcgcagcca tttttcatct atgatgcggc gcaacatgct ctgggcgtcg tggtagaggc
    14761 ggcgcgcttc tgtacccaca atttcgtcgt cgaggatggc agggaatttg ccggctaatt
    14821 cccaggtggc aaaaaacggc gtccagtcta tgtactcggt cagttcctcc aatggccatg
    14881 cctcaacgtg ctgaacgccg ggctgagccg gaacgggcgg cgtgtactgc tcccagtcgg
    14941 gccggaagcg attggcacgg gcggcttcga tgctgaggaa atttttggcc gcctttctnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccacg tgcaccattt
    15241 catccaaaga aggcgtgatg aggccgctca atccgatgat gtccacccgg tgttggcggg
    15301 ctgcatcgag gatggcggcg gcgggtacca tgacgccgag gtcaatgatt tcatagttgt
    15361 tgcagcccaa taccacgccc acaatgtttt tgccgatgtc gtgtacgtca cctttcacgg
    15421 tggccatcag gatgcggcct ttggcgcttt ggtcgcctga agcctgtttg tcggcttcga
    15481 tgaaaggctg gaggtaggct actgcctttt tcatcacacg ggcgcttttg accacctgcg
    15541 gcaaaaacat cttaccagcg ccgaacaggt cgcccaccac gttcatgccg tccatgagcg
    15601 gcccttcgat gacggccaaa ggcgaggaat aactcaggcg ggcttcttcc acatcgtcgt
    15661 caatatggtc ggtaatgcct ttgaccaagg cgtgcgagag gcggctttcg agactttccg
    15721 aacgccaggc agtatctttt tgcaacaccc tgccgccgcc cttgatgcgc tcggcgtagt
    15781 cggtgagggc ttcggtggct ttggggttgc gattgagcag cacgtcttct accaattgca
    15841 ggaggtcttt gggaatctcc tcatacacct caatcatgcc cgcattgacg atgcccatgt
    15901 ccatgccggc ctgaatggcg tggtacaaaa acgccgagtg catggcttcc cgaacgcggt
    15961 cgttaccccg aaaggagaag gaaatattgc tcacgccgcc gctgattttt gcgcctgggc
    16021 aatgctgctt aatgagtcga gtggcctcta tgtagttgat tgcgtactgg ttatgctcct
    16081 cgatgccggt ggccacggca aaaatattgg gatcgaaaat gatgtcgtgc ttattgaagc
    16141 caactttttc aattaaaatc ttataggcgc gtccacatat ttcaatgcgt ctttcatagg
    16201 aatctgcttg accatcttca tcaaaagcca tcacgataac cgcggctccg tagcgcttga
    16261 ctttcttcgc cttctctata aaatcagctt cgccttcttt cagcgaaata gaattaacta
    16321 cgcatttgcc ttgcacgcat ttcagacctg cttcaagaat ttcccacttg gaggaatcta
    16381 tcataatagg aatccgcgca atgtccggtt cgcttgcaat caggttgnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgata
    16561 agattcaaaa aagccttgga accagtcaca ttgcaacgct ctcctacgtt gatgaagttg
    16621 ctttcattgg ttgcgatgag cggctcgaga ccagagagct tcaatttata gcctttagcc
    16681 tttagctctt ggcctttagc taattcgaag tccacttcaa ttatattatc tttcttattc
    16741 aattgtgttc cgtttttatt ttagtaataa gagaattaat cattctctgt tcctcatgaa
    16801 tcaaggtaat tagtgaaatg aaattttcgc tatctgattt cattaaccct atttcttcaa
    16861 taatgattaa ttgcgtttct aattcaaaag ccgagccctg tgctatttcg agaaaacgct
    16921 tgaaatcaat ttcgccattt ctgctgcatc cttcggctat gtttgaggga attgatacag
    16981 ccgctctgtt catttgagaa acaatgccat acttttcttc cttgggtaac aaggaagtca
    17041 gcctataagt ggctttcact atctgcatac cttgttgcca tattttcaat tctctaaaat
    17101 tcctcatcga atttcaattc ataaatcacc tccaatagct aacagctaaa ggccaacagc
    17161 caagggcttt gtaattctag gtgcataatt ctttgctacc tgcgcaatgg ctttgatatg
    17221 atcaggagtt gttccgcaac aaccgcctac aatgtttatc aaaccctttt caaaatatgg
    17281 tttcatgagt tgagccatgt cttcagccgt ttggtcgtat tggcccatcg catttggtaa
    17341 gcctgcattg gcataagcac tcaccaaaca atcggcatta tcgctcaata tttctacata
    17401 ttgcatcaat gcttctgcgc ccatagcgca gttgaaacca atactcaaca atgggacatg
    17461 agatacagaa attagaaatg cctcggtagt ttgtcctgat aagattcttc cgcttgcatc
    17521 ggtaattgtt cctgaaatca tcacaggaag ttctttgccc gtttcttcaa aatactcctg
    17581 aatggcatac aaacaagcct ttgcattaag tgtatccgta atggtctcta tcaagagcaa
    17641 atccactccg ccttctacta aagcttgtac ttgttgctta aatgcaacaa ccaattcatn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat caggtgacat cgaagaaagc
    17761 tttgttgttg gtcctatgga accagctaca aaccttggct tagcaggatt ctttgccgtg
    17821 tattcatcgg ccacctctct tgcaattttc gcagaatagt aattcaaatc atacactaaa
    17881 tgctcgaggc cataatccgc ttgcgagaca gtagttccac taaaagtatt ggtttcaatg
    17941 atgtccacat cagcatcgaa atacgcagca tgaatggctt tgataatatc cggacgagtg
    18001 atactcaaca aatcg
//
LOCUS       DAORLF010000036        17653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1718_length_17653_cov_2.149335, whole genome shotgun sequence.
ACCESSION   DAORLF010000036 DAORLF010000000
VERSION     DAORLF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1718_length_17653_cov_2.149335"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK198_05190"
     CDS             complement(<1..228)
                     /locus_tag="PK198_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38160.1"
                     /translation="MENKRPGPFRRSLVFSLQLTALISVLHVVRVATTFPPLSWGILP
                     RDTDGLIGVVFAPLLHGSWGHLFSNMPPLAAL"
     assembly_gap    297..481
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<482..>1108)
                     /locus_tag="PK198_05195"
     CDS             complement(<482..>1108)
                     /locus_tag="PK198_05195"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRF38161.1"
                     /translation="QGMIENDFMDMNAGSVSNIIQTGGTILRSARSDAFRTPEGRRQA
                     YENLKAKGIEGIVAIGGDGTFTGANIFMKEYPDLAIVGCPGTIDNDLYGTDYTIGYDT
                     AINTAMAAIDNIKDTANAHGRLFFVEVMGRDAGFIALMSGLATGAEDILVPEFPTDMD
                     KLVKDLEDGFTNKKNSSIVVVAEGDDVGGAFHVAAEVKKRLVTPVEIK"
     assembly_gap    1109..1118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1119..1193)
                     /locus_tag="PK198_05200"
     CDS             complement(<1119..1193)
                     /locus_tag="PK198_05200"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38162.1"
                     /translation="MNACIRAVVRTALHAGIEKVSGIYR"
     gene            complement(1271..>1790)
                     /locus_tag="PK198_05205"
     CDS             complement(1271..>1790)
                     /locus_tag="PK198_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38163.1"
                     /translation="PAPVVQPASAPPVSEVAVTEVPPAPAPVQPEPVRAPVEAPAPQP
                     QVVVPPPPPKPQPAPPVQPAPTPRAAGAYEGLFSTKSSNDLSDKLSKQPIADLTKAFA
                     INDKLLYANDLFGKDQNAFAENVRQLNVLGNFESAKAHLIQLAQQHRWMDEERVETAQ
                     AFVQLVQRRYLA"
     assembly_gap    1791..1860
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2114..2207
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2526..3500
                     /locus_tag="PK198_05210"
     CDS             2526..3500
                     /locus_tag="PK198_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRF38164.1"
                     /translation="MKHHIFYLLIAAFCTASCDNLIRNPQGAVVDERDTMTSAPPETV
                     PQQQEETAMNDSDPHPDLIGFWVGYFLNAADDDYYEKSVYGDEGLSWGRENKINISID
                     EIHQGRVKGHSVVAGNNRPFAGTMTQEGNQFIFEVAEPGDDRYDGRFSFHIQKNDDKL
                     TGTWNAYKKIDIPTRKYELQKKRFQYDPELMLEPSRRYGDWGKAKKTAIEDEEEREAF
                     GDFYVQFEAATDKVYEVNASTTLLTKKDVENLKKGDLLIIRNTIYARHGYSFKNRPLR
                     VFFDAQPWYIPVHTDIRSDFTELEKKNIELLLRYEKNAKVYYDTFGRG"
     gene            3578..4141
                     /locus_tag="PK198_05215"
     CDS             3578..4141
                     /locus_tag="PK198_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRF38165.1"
                     /translation="MVLITIQYIPIDYEAAFLALKQDEIQLTHYRVAFFTHVYSSIFV
                     LLLGAFQFSGIIRRHYSLIHKAFGKAYVALILLFAAPSGLIMGYYGNGGFYAQAAFCL
                     LSALWFLFTLKAFLAIRARRIQVHQNFMILSYALTLSAISLRLFKWLIANTLALPPMD
                     TYKIVVWLGWIFNLAVALLLIKAKQRV"
     gene            complement(4265..>6208)
                     /locus_tag="PK198_05220"
     CDS             complement(4265..>6208)
                     /locus_tag="PK198_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF38166.1"
                     /translation="ARKGLADTALKTADAGYLTRRLVDVAQDVVILETDCNTLRGIET
                     SALKDNEKVIEPLSSRIEGRYTLHDIVHPETDAVILKAGEFIDGKASEYIEKVGVESV
                     TIRSVLTCETKRGVCAKCYGRNLATGRITENGDAVGIIAAQSIGEPGTQLTLRTFHVG
                     GIASVSKTESEITGKFNGRIEFDGMRITEYVDEEGGVIDIALSRTGEIRIVEGPNNKL
                     LASHYIPYGASVHVKDGQEITRGQLICEWDPFNAMIVSEYSGITKFKDVEEGVTFRVE
                     RDDQTGFAEKVVIESKNKKKVPVIQIVSPDGEELKSYTLPVGAYIAIDDETEVRAGTK
                     IVKIPRKLGKIQDITGGLPRVTELFEARNPSNPAIVSEIDGVVSFGKIKRGNREVAVV
                     AKDGQQRKYLIGLSKHILVQEGDFVRAGTPLSDGSIAPRDILAIKGPFAVQSYLVNGV
                     QEVYRSQGITINNKHIEVIVRQMMRRVQIEEAGDTHFLEGEAVEKYDFLETNDWIFDK
                     KFVIDAGESNRLKAGQLVTLRQVREENSFLKRNDKKAVTFRDAVAATSSPMLQGITRS
                     SLGTDSWISAASFQETTKVLSTAAIGAKRDDLNGLKENVIVGKRIPAGTGSRYLENFL
                     VTHKDNSHELELDHARYEDDMED"
     assembly_gap    6209..6232
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6233..6520)
                     /locus_tag="PK198_05225"
     CDS             complement(<6233..6520)
                     /locus_tag="PK198_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38167.1"
                     /translation="MGLITNNERYNQIIDKWTYADNRITDSLMRELSQHKQGFNSVFM
                     MLDSGARGSKQQIKQLCGLRGLMAKPRKSGDTGGAIIENPILSNFGDGLSVL"
     assembly_gap    6528..6572
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6573..8552)
                     /gene="rpoC"
                     /locus_tag="PK198_05230"
     CDS             complement(<6573..8552)
                     /gene="rpoC"
                     /locus_tag="PK198_05230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF38168.1"
                     /translation="MPFKKNQTQKQDFSGITISLASPDDILERSYGEVLKPETINYRS
                     YKPERDGLFCERIFGPVKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWFFRSLPNKIGYLLGLSSKKLESVVYYERYVVLQPGVSGKVLNELLTEEE
                     YMEVMDALPADNQSLDDSDPNKFVARMGAEAIRDMLMRLDLDQLSYDLRYQAANETSQ
                     QRKSEALKRLRVVEAFREGQQQMENRPEWMIIQYLPVVPPELRPLVPLDGGRFASSDL
                     NDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAEGGRA
                     LKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKTMAAELFKPFV
                     IRKLIERGIVKTVKSAKKLVDRKDSVIWEILENILKGHPVLLNRAPTLHRLSIQAFQP
                     KLIEGKAIQLHPLVCGAFNADFDGDQMAVHVPLSQSAILEAQVLMLASHNMLNPQDGS
                     PVTLPSQDMVLGLYYITKERKGTPETPVKGEGRKFYSSEEVVIAFNEGQLDLHAPIKV
                     RVNVEDENGVVARRLIDTTTGRIIFNQAVSDEVPYINQLLTKKNLKKVIGDVLRRTNF
                     KVTAEFLDNIKDLGFTWAFKGGLSFNLGDLILPSIK"
     gene            complement(8613..12431)
                     /gene="rpoB"
                     /locus_tag="PK198_05235"
     CDS             complement(8613..12431)
                     /gene="rpoB"
                     /locus_tag="PK198_05235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRF38169.1"
                     /translation="MASNGMVPTAEAKRVSFGKIRFPSPHPDLLEIQLKSFQEFFQLE
                     TTPENRISEGLYKVFQENFPITDARSIFVLEFLDYFVDPPRYSIEECMHRGLTYSVPL
                     KAKLRLSCNDEEHVDFQTIVQDVFLGNIPYMTPKGTFVINGAERVIVSQLHRSPGVFF
                     GQTFHPNGTAIYSARVIPFKGAWMEFATDINTVMYAYIDRKKKFPVTTLLRAIGYDTD
                     KAILDIFGLADEVPATREQLEKSVGRKLAARVLRSWSEDFVDEDTGEVVTIERNEIIL
                     ERDIILDEDNIERILDAETDVVILQKEDIDQDYSIIYNTLQKDPTSSEVEAVIYIYRQ
                     LRGTDPPDEETARGIIEKLFFSDKRYNLGEVGRFKINRKLGMETSMDTLVLTREDIIS
                     IVVYLVSLMNQKAEIDDIDHLSNRRVRSVGEQLYAQFGVGLARMARTIRERMNVRDNE
                     VFTPVDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERA
                     GFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINKMGFLETPYRAVTDGVVDG
                     SEVRYLSAEAEDFLKIAQANAPLDEKGAFVKDRVKSREHGDFPVLTPDELEYMDVAPN
                     QIVGVSASLIPFLENDDANRALMGSNMQRQAVPLIRPSSPIVGTGVEAKVALDSRMLL
                     NAEGEGVVEYVDANEIIIRYDRNEDEQLISFEDNRRTYKLTKFMSTNQGTCINLKPIV
                     RRGDRVYPGMVLCEGYATEKGELALGQNLKVAFMPWKGYNFEDAIVLSERVLHEDVFT
                     SIHIEHFEMDVRDTKLGEEELTNDIPNVSEDATKDLDENGIVRIGAWVKEGDILIGKI
                     TPKGESDPTPEEKLLRAIFGDKAGDVKDASLKTPPSISGVVIDKQLFARAKKDNKQKA
                     QEKDLLTKLDDQHEIALNELKTILIDKLQVLVKGKTSQGLRTIYNEQLVPKGVKFSLE
                     VLRDLDYGTVDYANWTEDAAANELIARLLHNYSIKVNEEVGRYKREKFNISIGDELPA
                     GVLKLAKVYLAKKRKLQVGDKLAGRHGNKGIVSRIVRIEDMPFLDDGTPVDIVLNPLG
                     VPSRMNLGQIFETVLGWSGEKLGVKFSTPIFDGATKDEIEEYIQKAGLPSLGQTYLHD
                     GETGDRFHQQATVGIIYMLKLSHMVDDKLHARSIGPYSLITQQPLGGKAQFGGQRFGE
                     MEVWALEAFGASHILQELLTIKSDDIAGRAKAYEAIVKGDNLPEPNVPESFNVLVHEL
                     RGLALDVKFD"
     gene            complement(12628..>12936)
                     /gene="rplL"
                     /locus_tag="PK198_05240"
     CDS             complement(12628..>12936)
                     /gene="rplL"
                     /locus_tag="PK198_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRF38170.1"
                     /translation="VLKTEYGIEPAAAAVAIAAPAADGGGAAPVVEKTEFDVALNSAG
                     ANKLQVIKLVKDKLGLSLGDAKALVDSAPCTLKAGMPKADAEALKATLVEQGAEVELK
                     "
     assembly_gap    12937..12960
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12961..13035)
                     /locus_tag="PK198_05245"
     CDS             complement(<12961..13035)
                     /locus_tag="PK198_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRF38171.1"
                     /translation="MADIKALAEQLVNLTVKEVSELADV"
     gene            complement(13161..13697)
                     /gene="rplJ"
                     /locus_tag="PK198_05250"
     CDS             complement(13161..13697)
                     /gene="rplJ"
                     /locus_tag="PK198_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRF38172.1"
                     /translation="MTKADKTAAIEALKEKLETANNFYLTDSSTLTVGQVNKLRKLCY
                     DNGIELHVVKNTLVRKAMDSFSEDRNYAALYDALHGPTAIMFSESAKAPATLIKQFRK
                     DHERPVLKAAYIDASVFMGDEQIEVLVKLKSKEELLGEVVMLLQSPARNVISALQSGG
                     QTIAGLVKALEERGGAAQ"
     gene            complement(13718..>14378)
                     /gene="rplA"
                     /locus_tag="PK198_05255"
     CDS             complement(13718..>14378)
                     /gene="rplA"
                     /locus_tag="PK198_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRF38173.1"
                     /translation="EDKVDRTKLYTIPEAMTLVKQVNTTKFDASVDIHVRLGVDPRKP
                     DQAIRGSVTLPHGTGKVKRVAVFCTPDKEAEAKAAGADYVGLEELIQKVQGGWTDVDV
                     VIAMPQVMGQVGRIGRILGPRGLMPNPKTGTVTQDVAAAVGDVKGGKISFRVDKYGII
                     HASVGRISFDPAKLADNANELLGALQRLKPSSAKGTYMKSVSVAGTMSPGIKVDIKTI
                     R"
     assembly_gap    14379..14453
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14484..14939)
                     /gene="rplK"
                     /locus_tag="PK198_05260"
     CDS             complement(14484..14939)
                     /gene="rplK"
                     /locus_tag="PK198_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRF38174.1"
                     /translation="MAKEIETFIKLQVKGGQANPAPPIGPALGSKGVNIMEFCKRFNA
                     VTQDKMGKIIPVIITVFKDKSFEFVLKTAPAAAQLMEVSKIKSGSAQPNRNKVGSVTW
                     DQVKAIAEDKMADLNCFSLDAAMSMVAGTARSMGLRVEGTPPWGDSDVN"
     gene            complement(15045..15593)
                     /gene="nusG"
                     /locus_tag="PK198_05265"
     CDS             complement(15045..15593)
                     /gene="nusG"
                     /locus_tag="PK198_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRF38175.1"
                     /translation="MSEEKKWYSLRVISGKERKIKERIELEIQREGWSEFITQVIVPT
                     EKVYKIRNGKKVISERNILPGYLLIEADPVRFTPEIVQAIANVPNVIHFLGKNTPIPM
                     REAEANRMLGKVDESQGAGESLIEPFIVGETIKIIDGPFSEFVGDIQEVNEEKKKLKV
                     IVKIFGRGTEVELNFMQVEKQA"
     gene            complement(15610..15807)
                     /gene="secE"
                     /locus_tag="PK198_05270"
     CDS             complement(15610..15807)
                     /gene="secE"
                     /locus_tag="PK198_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRF38176.1"
                     /translation="MEKIKLYLLESYNELVHKVTWPTMSSLMSNTVAVLVASAILMGV
                     IILMDGGSNALTKLIYGVGSN"
     gene            complement(15823..15895)
                     /locus_tag="PK198_05275"
     tRNA            complement(15823..15895)
                     /locus_tag="PK198_05275"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15860..15862),aa:Trp,seq:cca)
     gene            complement(15987..17183)
                     /gene="tuf"
                     /locus_tag="PK198_05280"
     CDS             complement(15987..17183)
                     /gene="tuf"
                     /locus_tag="PK198_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRF38177.1"
                     /translation="MAKEQFQRTKPHVNVGTIGHVDHGKTTLTAAITYVLSQSGLAEK
                     KDYDSIDAAPEEKERGITINTAHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDLVDDPEMLELVEMEVRELLDT
                     YGFDGDSASIIKGSALKALEGNPDGERAIKELMDAVDNAIPQRERLVDKPFLMPVEDV
                     FSITGRGTVATGRIERGVIKTGDPVEIVGMLKEGEKPLTSTVTGVEMFRKILDRGEAG
                     DNAGLLLRGIEKEAIRRGMVICAPGSVKPHKKFKCEVYVLSKEEGGRHTPFFNGYRPQ
                     FYFRTTDVTGDCKLAEGVEMVMPGDNVTMEVSLLNTIAMEKGLRFAIREGGRTVGAGQ
                     VTEIID"
     gene            complement(17234..17305)
                     /locus_tag="PK198_05285"
     tRNA            complement(17234..17305)
                     /locus_tag="PK198_05285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17271..17273),aa:Thr,seq:ggt)
     gene            complement(17367..17439)
                     /locus_tag="PK198_05290"
     tRNA            complement(17367..17439)
                     /locus_tag="PK198_05290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17404..17406),aa:Gly,seq:tcc)
     gene            complement(17450..17535)
                     /locus_tag="PK198_05295"
     tRNA            complement(17450..17535)
                     /locus_tag="PK198_05295"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17498..17500),aa:Tyr,seq:gta)
BASE COUNT         3992 a         4433 c         4341 g         4360 t
ORIGIN      
        1 gagggcggcc aatggcggca tattggaaaa caggtgcccc caactgccat gcaacaatgg
       61 cgcaaaaact acaccgatga ggccgtcggt atcgcgtggc aaaatacccc aggacaaggg
      121 cggaaacgta gtggctacgc gcaccacatg cagcaccgag atcagtgccg tgagttggag
      181 cgaaaacacg agactgcgcc ggaagggtcc gggacgttta ttttccatag cttgttgttt
      241 ttgacgccca acatcaggcc agcacccgaa tcatttccat gatgccgggg tctattnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nctttaattt ctaccggcgt aacgagacgc tttttcacct cagccgccac atgaaatgct
      541 ccgccgacat catcgccttc agcaaccacc acgatgctgg agttcttttt attggtgaag
      601 ccatcttcca gatcctttac caatttgtcc atatccgtcg gaaactccgg caccaagatg
      661 tcttcggcgc cggtagccag gcccgacatg agggcaataa aaccggcatc tcggcccatg
      721 acctctacaa aaaacaagcg cccgtgcgca ttggcagtgt ctttgatgtt gtcaatggca
      781 gccatggccg tattgatggc agtatcgtag ccgatggtat aatcggtgcc gtacaggtcg
      841 ttgtcaatgg tgccgggaca accgacgatg gccaaatcgg ggtactcctt cataaaaata
      901 ttggccccgg taaaagtgcc gtcgccgccg atggctacaa tgccctctat gcccttggcc
      961 ttgaggtttt cgtaagcctg ccggcgacct tcgggtgtgc gaaaggcatc gctgcgcgcc
     1021 gaacgaagga tggtgccacc cgtctggatg atgttgctga cactgccggc attcatatcc
     1081 atgaagtcgt tttcgatcat gccctgatnn nnnnnnnncc ggtagatgcc actgactttt
     1141 tcaatgcccg cgtgcagggc cgtgcgtact actgcgcgga tgcaggcgtt catgcctggc
     1201 gaatcgccgc cggaggtaaa aacagctata tgtttcattg tttttttgga tttcgtagtg
     1261 tgttttatac tcaggccaag tatcggcgct gaaccaattg cacaaaagcc tgggccgttt
     1321 ccacgcgctc ttcatccatc caccggtgct gctgcgcgag ttggatgaga tgcgctttgg
     1381 cgctttcaaa attgccgagc acgttcaact gccgaacgtt ttctgcaaag gcattttggt
     1441 ctttcccaaa caaatcattg gcgtacagca acttgtcgtt gatggcaaat gccttggtga
     1501 ggtcggcaat cggctgtttg ctcagtttat ccgacaggtc attggacgac ttggtactga
     1561 acaagccttc gtaggcgccg gcagcgcggg gcgtcggagc gggctgcacg ggaggagcag
     1621 gctgcggctt gggcggcggc gggggtacta ccacttgcgg ttgaggggca ggcgcttcaa
     1681 caggggcgcg cacaggctca ggttgcacag gggccggagc cggaggaact tccgtcaccg
     1741 ctacttctga cacaggcggc gcggatgcag gctgcacaac cggcgcgggt nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 aactggttga aatcgagaaa tacattatac agttgccgga tgtagctgag catgagatcg
     1921 cgctcgatgg ccgataccgg gccgggagta gcgttgatac tgcgccacag ggcattgatt
     1981 ttttccagca aaatctgagc ttgttgagct tccatgttga tttttacaaa atgatggtac
     2041 aaaaacaatc gggcgcaaag ataaagtatt ggtaactact tagcatcttt ttcaaggcca
     2101 caaccacgcc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct tctccttttg
     2221 gaaaaccacc acgcagggcg tggttgtagc taatagctaa aggccgatgg aaaatggtaa
     2281 atagatatct cattgaaaaa gaattcttgt ctttttggaa aatcaaccac acaggatttg
     2341 ggtgcggcta agctcatttt cctcccgctc actttgatgc tcatccccac cgtgttgcaa
     2401 cccggtttga cccggctggg gtactcactt aaaataatgg aaaccaaatc tttgtaataa
     2461 ttgcaacaac tataaccaac gtgtagaaat ttccctttaa tttactccgc aaaatgagca
     2521 ctcttatgaa acatcacatt ttttacctgt tgatcgcagc attctgtact gcaagctgcg
     2581 acaacctgat tagaaaccct caaggcgccg tggtggacga gagggacacc atgacctctg
     2641 ccccgccgga aacagtcccg cagcagcagg aggaaactgc catgaatgat tcagacccgc
     2701 accccgatct cataggtttt tgggtaggct attttctgaa tgctgccgac gacgattatt
     2761 atgaaaagtc tgtgtatgga gatgagggct tgagttgggg ccgggaaaac aaaatcaata
     2821 tttccataga tgaaattcat cagggccgcg tgaaaggaca cagcgtggtg gcaggaaata
     2881 accgcccctt tgcgggcact atgacgcaag agggaaacca atttattttc gaggtcgcag
     2941 agccgggaga cgaccgatac gacgggcgtt tttcttttca cattcaaaaa aacgacgaca
     3001 agctgacagg cacctggaat gcctataaaa agattgacat tcccacaaga aaatacgagt
     3061 tgcaaaaaaa acgcttccaa tatgaccctg aactcatgct ggagccatcg cggagatacg
     3121 gcgactgggg gaaagcgaaa aaaacggcca tagaggatga agaagagcgc gaggcattcg
     3181 gagattttta tgtgcagttt gaggcggcta cggataaggt atatgaagtg aatgcttcta
     3241 ctaccttgct cacaaaaaaa gatgtggaga acttaaagaa aggcgatttg ctcatcatcc
     3301 gcaatacgat ttatgcaagg cacggctact cttttaaaaa caggcctctg cgcgtttttt
     3361 tcgatgctca gccctggtat attcccgtgc atacagacat tcggagcgat ttcacagaac
     3421 tggaaaagaa aaatatcgag ttgctgctga gatatgaaaa aaatgccaaa gtgtattacg
     3481 atacattcgg ccggggataa aaatgaatac gcgccactac tatcaatggg gaggcaaagt
     3541 gcttctgtat ggggttttgt tctttttcat ttaccttatg gtattgatta ccatacagta
     3601 tattcccatt gattatgagg ccgcatttct ggccttaaag caagatgaaa tccagttgac
     3661 gcattaccgg gtagcatttt tcacgcatgt ctattccagt atttttgtac tcctattggg
     3721 agcctttcag ttttccggga ttattcgaag acattattct ttgattcaca aggcattcgg
     3781 aaaggcgtat gtggcgctta ttctgctttt tgcggcgcct tcgggactga taatggggta
     3841 ttatggcaac ggcggctttt acgcgcaggc ggctttttgc cttttgtctg ctctgtggtt
     3901 tctctttacc ctaaaggctt ttttggctat aagagcgcgg cggattcagg tccatcagaa
     3961 ttttatgatt ttgagctatg cactgacact ttcagccatc agtttgcgcc tgtttaaatg
     4021 gttgatagcc aatacactgg cactaccacc gatggacacc tacaaaattg ttgtgtggtt
     4081 gggatggata ttcaatctcg cggtagcact tttactgatc aaagcaaaac aaagggtttg
     4141 agcgtaatgc cgctttgttg gccatcaaaa cacgctgcca taatatttac acaagcaaaa
     4201 aaaatccccc acggagtatt gaattgctcc gaaggggatt ttttaaaacc aatatcctca
     4261 gaaattaatc ttccatatcg tcttcgtagc gggcatgatc caattccaac tcgtgggaat
     4321 tgtctttgtg cgtcaccaag aagttttcca gatagcgcga tcccgtacct gccggaatgc
     4381 gcttgcctac aatgacgttc tctttcaggc cgttgaggtc gtcgcgcttg gcgccgatgg
     4441 cagccgtgct gagtactttg gtggtttcct ggaacgaagc cgccgaaatc caactgtcgg
     4501 tacccaatga agaacgcgtg ataccctgca acatcgggct ggacgtagcc gctacggcat
     4561 cgcggaacgt aaccgctttt ttgtcgttgc gcttgaggaa ggagttctcc tcgcgcacct
     4621 gacgcagcgt aacgagctgt ccggccttga ggcggttcga ttcgccggca tcaatgacga
     4681 atttcttatc gaaaatccag tcgttggttt ccagaaaatc gtatttctcc actgcttcgc
     4741 cttcgaggaa atgcgtatcg ccggcctctt caatttgtac gcggcgcatc atctggcgca
     4801 cgatcacttc aatatgcttg ttgttgatcg taataccctg agaacgatac acttcctgta
     4861 cgccgttcac taagtaagac tgcacggcaa acgggccttt gatggcgaga atgtcgcggg
     4921 gcgcaatgct gccgtcggac agcggcgtgc cggcgcgcac aaagtcgcct tcctgcacca
     4981 gaatgtgctt ggacaagccg atgaggtact tgcgttgctg accgtctttg gctactacgg
     5041 ctacttcgcg gttgccgcgc ttgatcttgc cgaaagatac cacgccgtca atttcggaga
     5101 cgatggccgg attcgacggg ttgcgcgctt cgaagagttc ggttacgcgc ggcagaccac
     5161 cggtgatgtc ctgaatcttg cccagcttgc ggggaatctt cacgattttc gtaccggctc
     5221 ttacctccgt ttcgtcgtca atggcaatgt aagcgcccac aggcagcgta tagctcttca
     5281 gttcttcgcc atcggggctg acgatctgaa tgacgggtac ttttttcttg ttcttcgatt
     5341 cgatcaccac cttttcggca aaaccggttt ggtcgtcgcg ctcaacgcgg aaggtcacgc
     5401 cttcttcaac gtctttgaac ttggtaatac cggagtattc cgaaacgatc atggcgttga
     5461 aggggtccca ttcgcagatc aactgcccgc gcgtaatctc ctgaccgtct tttacatgta
     5521 cggaagcgcc gtacggaatg tagtgcgatg ccagcagttt attgttcggg ccttccacaa
     5581 tacggatttc accggttctg gaaagtgcaa tatcaattac gccgccttct tcgtccacat
     5641 attcggtgat acgcataccg tcgaactcaa tgcggccatt gaacttaccc gtgatttccg
     5701 actcggtttt ggacaccgat gcaataccgc ccacgtggaa ggtacgaagg gtgagctgcg
     5761 tacccggttc accgatagac tgcgccgcaa tgatgcccac cgcatcgccg ttttcggtaa
     5821 tacgccccgt agccaggttg cgaccatagc acttggcgca cacgccgcgc ttggtttcac
     5881 aagtgagcac agagcgaatg gtaacagatt ccacacctac tttttcgatg tattccgaag
     5941 ccttgccgtc aataaactct ccggctttca ggatcaccgc gtcggtttcc gggtgtacaa
     6001 tatcgtgcaa cgtgtagcgg ccttcgatgc gggaagacag cggctcgatg actttttcat
     6061 tgtctttgag cgcagatgtt tcgataccgc gcaaggtgtt gcagtccgtt tcaaggatga
     6121 ccacgtcttg cgccacgtcc accaaacgac gggtaaggta acccgcgtcg gcagttttca
     6181 gagccgtgtc ggccaaaccc ttgcgggcnn nnnnnnnnnn nnnnnnnnnn nncaataccg
     6241 agaggccgtc gccaaagttg gagagaatcg ggttctcgat gatggcgccg ccggtatcgc
     6301 cgctcttacg aggcttggcc ataaggccgc gaagcccgca caactgcttg atctgctgtt
     6361 tggaaccacg cgcgccggag tcgagcatca taaacacaga gttgaagccc tgcttgtgct
     6421 gcgatagttc gcgcatcaag gaatcagtga tgcggttgtc ggcataggtc cacttgtcaa
     6481 tgatctggtt gtagcgctcg ttgttggtaa tgagacccat gttgtagnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttgatgg agggcaaaat gaggtcgccg
     6601 aggttgaacg acaagccgcc tttgaaagcc caggtaaagc ccaggtcctt gatgttgtcg
     6661 aggaattcgg cagttacctt gaagttcgta cggcgcagta cgtcgccgat gacttttttc
     6721 aggtttttct tggtcaacaa ctgattgatg taaggcactt catcagacac ggcctgattg
     6781 aagatgatgc gaccggtagt cgtatctatc aaacgccttg caacaacgcc gttttcatct
     6841 tccacattca cccgcacttt gatgggcgcg tgcaggtcga gctggccttc gttgaatgca
     6901 atcactacct cttcagacga atagaacttc cggccttcgc cctttacagg cgtttcggga
     6961 gtacctttgc gctctttggt gatgtagtac aagcccaata ccatgtcctg tgaaggcagc
     7021 gtaacgggcg aaccgtcttg cgggttgagc atgttgtgcg aagccagcat aagcacctgc
     7081 gcttcgagta tggccgactg gctgagcgga acgtgaaccg ccatttggtc gccgtcgaag
     7141 tcggcgttga acgcgccgca caccaagggg tgcaactgaa tggccttgcc ttcgatgagt
     7201 ttgggctgga atgcctgaat agaaaggcgg tgcagcgtcg gggcgcggtt gaggagcacc
     7261 gggtggcctt tgaggatatt ttcgaggatt tcccagatga cggaatcctt gcgatccacc
     7321 aatttcttgg ccgattttac ggttttcaca atgccgcgct cgatgagctt ccgaataacg
     7381 aatggcttga acaattcagc cgccatcgtt ttgggaatgc cgcactcatg gagcttcagg
     7441 gtcgggccta ccacgatcac agaacgaccg gagtagtcca cacgcttacc caacaggttt
     7501 tgacggaaac ggccctgctt gcctttaaga atatcgctca aagacttgag cgcccgaccg
     7561 ccctcagcct tcacggcatt ggatttgcgg gagttgtcga acagcgagtc cacggcttcc
     7621 tgcaacatcc gcttttcgtt gcgcaagatc acttccggag ctttgatttc cagaaggcgc
     7681 ttgaggcggt tgttgcggat gataaccctg cgatagaggt cgttgaggtc ggaactggca
     7741 aagcggccgc catccaaagg caccaagggg cgcagttcgg gcggcactac cggcaggtat
     7801 tggatgatca tccattccgg gcgattctcc atctgctgct gaccttcccg gaaagcctcc
     7861 accacgcgca aacgcttgag tgcttccgat ttgcgctgct gcgaagtctc attggctgcc
     7921 tgatagcgca aatcatacga aagctgatcc agatcaaggc gcatgagcat atcgcgaatg
     7981 gcctcggcgc ccatgcgggc aacgaattta ttcgggtcgc tgtcgtctaa gctttgattg
     8041 tctgccggca aagcgtccat cacttccatg tactcttcct cggtgagcaa ctcattgagg
     8101 accttgccgg aaacacccgg ctgcaacacc acatagcgct cgtaataaac aaccgattcc
     8161 aattttttgg aagacagccc caacaggtaa ccgattttgt tgggcaagga acggaagaac
     8221 caaatgtgta ccaccggcac caccaacttg atgtggccca tgcgttcgcg gcgcactttt
     8281 ttctccgtta cttctacgcc gcaacggtca caaacgattc ctttgtaacg aatgcgtttg
     8341 tatttgccgc agtagcactc gtagtccttt accgggccga atatccgctc acaaaacaga
     8401 ccatcgcgct ccggtttgta agagcggtag ttgatcgttt cgggtttgag cacttctcca
     8461 taagagcgct ccaaaatatc gtccggcgaa gcaaggctga tagtgatgcc actaaaatcc
     8521 tgcttttggg tctgattctt tttgaaaggc atattgctgg cgagttgaaa aagattttca
     8581 aaaaaattga ttactcagtg aaggccgatt catcaatcga acttcacatc caatgcgagg
     8641 ccgcgcaatt cgtggaccaa tacattgaat gattccggta cgttaggctc cggcaggttg
     8701 tcgcctttca cgatggcttc gtaagccttg gcgcgccctg caatgtcgtc cgatttgatg
     8761 gtgagcaatt cctgcagaat gtgcgatgca ccgaaagctt cgagcgccca cacttccatc
     8821 tcaccaaaac gctgaccacc aaactgcgct ttaccaccca gaggttgctg cgtgatgagc
     8881 gagtacgggc cgatggagcg ggcgtggagc ttgtcgtcca ccatgtgaga gagtttcagc
     8941 atgtaaatga tgcccaccgt ggcctgctgg tggaaccggt cgccggtttc tccgtcgtgc
     9001 aggtaggttt gtcccaggct gggcaatcct gctttctgga tgtactcttc gatctcgtct
     9061 ttggtggcgc cgtcgaagat gggcgtagag aattttacac ccaatttttc gcccgaccaa
     9121 cccagtacgg tttcaaaaat ctggcccagg ttcatacgcg aaggtacgcc cagcgggttg
     9181 agcacaatgt ccacaggcgt tccgtcatcc aggaaaggca tatcttcgat gcgcacgatg
     9241 cgagacacga tgcccttgtt gccgtgacgt ccggcgagct tgtcgcccac ctgaagtttg
     9301 cgcttcttgg cgagataaac tttggccaat ttgagtacgc cggcgggcaa ttcatcacca
     9361 atgctgatgt tgaatttttc gcgcttgtag cggcctactt cttcattcac tttgatgctg
     9421 tagttgtgca gcaatcgggc gatgagttcg ttggcggcgg cgtcttccgt ccagttggcg
     9481 tagtccaccg ttccatagtc cagatcgcgc agtacctcca aagagaattt gacgcccttg
     9541 ggtaccaatt gctcgttgta aatggtgcgc aagccctgac tcgttttgcc tttcaccaac
     9601 acttgcaact tatcaatgag aatggttttc aactcattga gcgcaatctc gtgctggtcg
     9661 tccaacttag tgagcaggtc tttttcctgc gccttttgct tgttgtcttt tttggcgcgc
     9721 gcaaacaact gcttgtcaat caccacgcca ctgatggacg gcggtgtttt gagcgaagca
     9781 tccttcacgt cgccggcttt gtcgccgaag atggcacgaa ggagtttttc ctccggtgtc
     9841 gggtcggatt cgcctttcgg agtgatctta ccgatgagga tatcgccttc tttcacccag
     9901 gcgccgatgc gaacgatacc gttttcatcg aggtctttgg tggcgtcttc gctcacgttg
     9961 ggaatgtcgt tggtcaattc ctcttcaccg agtttggtat cgcgcacatc catctcgaag
    10021 tgttcgatgt ggatggaggt aaatacgtcc tcgtgaagta cgcgctccga caacacaatg
    10081 gcgtcttcaa agttgtaacc tttccagggc atgaaggcca ctttgaggtt ttgccccagt
    10141 gccaattcgc ccttttcggt tgcgtaacct tcacacagca ccatgcccgg atacacgcga
    10201 tcaccccggc gcacaatcgg cttgaggttg atgcaggtac cctggttggt actcataaac
    10261 ttggtgagct tgtaagtacg gcggttgtct tcaaaagaga tcaactgctc gtcttcgttg
    10321 cggtcgtagc ggatgatgat ttcgttggcg tccacatact ctactacgcc ctcgccttct
    10381 gcgttgagca acatgcggga gtcaagcgcc actttggcct ccacccccgt acccacgatg
    10441 ggagagctgg ggcgaatgag cggtaccgcc tgacgttgca tgttcgatcc catgagggcg
    10501 cggttggcgt catcgttttc gaggaaggga atcaaagaag ccgatacgcc cacaatctgg
    10561 ttgggcgcca cgtccatata ttccaactca tcaggcgtca acaccgggaa atcgccatgt
    10621 tcgcggcttt tcacgcggtc ttttacaaag gcgccctttt cgtccaatgg agcattcgcc
    10681 tgggcaatct tgagaaagtc ttctgcttct gccgacaggt aacgcacttc gcttccgtcc
    10741 accacgccat cggttaccgc acggtaaggc gtttccagaa agcccatttt attgatcttg
    10801 gcgtgtacgc acaaggtaga gatcaaaccg atgttcggac cttcaggcgt ttcgatggta
    10861 cacaaacggc cgtagtgcga atagtgtacg tcgcgcacct cgaagccggc gcgttcgcgc
    10921 gaaagaccgc ccgggcctaa ggccgaaata cgacgcttgt gcgtgatctc ggaaagcggg
    10981 ttggtctgat cgaggaactg agacaactgg ctggtaccaa agaaggagtt gatgacggaa
    11041 gaaagcgtgc gggcattgat gaggtccacc ggagtaaaca cctcgttgtc gcgcacattc
    11101 attctttcgc ggatggtgcg ggccatacga gccagaccaa cgccgaactg agcatacaat
    11161 tgctcgccta cgctgcgtac gcggcggttg ctcaagtggt caatatcgtc aatctcagcc
    11221 ttctggttca tcaggctcac caaatacacc acgatggaaa taatatcctc gcgggtgagc
    11281 accaatgtgt ccatgctggt ttccatgccg agcttgcggt tgatcttgaa acggcctact
    11341 tcgccaaggt tgtagcgctt gtcggagaag aacaattttt cgatgatacc gcgagccgtt
    11401 tcttcgtctg gcggatcagt accgcgcaat tgacgataga tataaatcac tgcttcgacc
    11461 tccgaactgg tggggtcttt ttgcaatgta ttatagatga tagagtaatc ctggtcaatg
    11521 tcttctttct gaaggatgac cacatcggtt tctgcatcta agatgcgctc aatgttgtct
    11581 tcatccagaa tgatgtcgcg ctccagaatg atctcgttgc gctcaatggt taccacctca
    11641 ccggtatctt cgtccacgaa gtcttccgac cagctacgca gtacccgggc agccaacttg
    11701 cggccaacgg atttttccaa ctgttcgcgc gtggccggca cttcatcggc caaaccgaaa
    11761 atatcgagga tggctttgtc tgtgtcgtaa ccaatggcac gcagaagggt ggttaccggg
    11821 aacttcttct tgcgatcaat gtacgcatac atgaccgtgt tgatgtcggt agcaaattcc
    11881 atccaggcgc ctttaaaagg aataaccctg gcagaataga ttgccgtgcc gttcgggtgg
    11941 aaagtctgac cgaaaaatac gcccggagag cggtgcaact gagaaacgat cacccgctcg
    12001 gcgccattga taacaaaggt gcctttgggc gtcatgtaag ggatgttccc aaggaataca
    12061 tcctgtacta tcgtttggaa atcaacgtgt tcctcatcgt tgcaggaaag acgcaacttg
    12121 gctttgagag gtacgctgta ggtcagccca cggtgcatac attcttcgat ggagtaacga
    12181 ggcggatcaa caaagtaatc caaaaactcc agaacgaaga tgctgcgagc atccgtgatg
    12241 gggaagtttt cctgaaaaac cttatacagg ccttcgctga tccggttttc aggagtagtt
    12301 tccaattgaa agaactcctg aaacgatttg agctggattt caagcaaatc ggggtgagga
    12361 gaagggaatc tgattttgcc gaagcttacg cgtttagctt ctgccgtagg aaccatgccg
    12421 tttgatgcca tttgcaaatg atattttatt cccggatatg ttaaagtgcc ttttcaggca
    12481 aaatgttcag cctgtgccga actttcgtca tttctttcaa agacgacaat aggcttaacg
    12541 gatctaaaca gacccgttaa gcctttttgt tgtcgaacaa actgacaatc aagtacttga
    12601 ctgcctgaga gccgacggag gaactgctta cttcagctca acctctgcgc cctgctctac
    12661 cagagtggct ttcagagctt cagcgtcagc tttgggcata cctgccttca gggtgcaagg
    12721 agcgctgtca accagtgctt ttgcatcgcc gaggctgagg cccaacttgt ctttcaccaa
    12781 cttgatgacc tgcaacttgt tggcgcctgc tgaattgaga gctacgtcaa actccgtttt
    12841 ttcaacaacc ggagcagcgc cgccgccatc agcagccgga gcagcaatag ctacagcagc
    12901 agcagccggc tcgatgccgt attctgtctt aagaacnnnn nnnnnnnnnn nnnnnnnnnn
    12961 aacgtctgcc aattcgctta cttcttttac cgtaaggttg accaactgtt ctgcgagggc
    13021 tttgatatct gccatttttc ttaaattttg aaaaggtgaa acgataaaat tacttttatc
    13081 gtgcgtttga acttggaagc cgaatcaatg tggcggcccg ctatcggagc agtggctccg
    13141 gacaggctgc cgggtaaaaa ttactgagct gcgccgccgc gttcttccaa cgctttgact
    13201 aagccggcaa tcgtctgacc accagactgc aatgcactga tgacattgcg tgccggagat
    13261 tgcaacaaca taaccacttc gcccaacaat tcttccttgg acttgagctt gaccagcact
    13321 tcaatctgct cgtcacccat aaatacgctg gcgtcaatgt acgcagcttt cagaacgggg
    13381 cgctcgtggt ctttgcggaa ttgcttgatg agtgttgcgg gcgctttggc agactccgaa
    13441 aacataatcg ccgtagggcc atgcagtgca tcgtacaatg cagcatagtt gcggtcttca
    13501 gagaaactgt ccattgcttt gcgcaccaaa gtgtttttaa caacatggag ttcgatcccg
    13561 ttgtcatagc acagtttgcg cagtttgttc acctgtccca cagtcagtgt ggacgaatcg
    13621 gtgaggtaaa aattattggc tgtttccagc ttttctttca gcgcctcaat ggctgctgtt
    13681 ttatctgcct tcgtcatggc ttcgacattt tttaagacta gcgaatcgtt ttgatgtcca
    13741 ctttgatacc cgggctcatc gtgccggcca cactcactga tttcatgtaa gtgcctttgg
    13801 cagaggaggg cttgaggcgt tggagtgccc cgagcaactc attggcgtta tctgccaatt
    13861 ttgcgggatc aaaagaaata cgaccgacgg aagcgtgaat gataccatat ttatctacgc
    13921 ggaaagaaat tttaccgcct ttgacatcgc cgacagcagc cgcgacatcc tgagtaacag
    13981 tacccgtttt ggggttgggc atcaaaccac gcgggccgag gatacggccg atacgaccta
    14041 cctgacccat gacctgcggc atagcaatga ctacgtccac atctgtccaa ccgccttgta
    14101 ctttctggat caattcttcc aaaccaacat agtcagctcc ggcagctttg gcttcggctt
    14161 ctttgtcggg agtacagaat acagctaccc ttttcacttt accggtgccg tgaggcaggg
    14221 taacagagcc gcgaatcgcc tgatcgggtt tgcgagggtc cacgcccaag cgaacgtgaa
    14281 tgtcaacgga agcatcaaat ttggtggtgt tcacctgctt gaccaaggtc atagcttccg
    14341 gaatggtgta gagcttggta cgatctacct tgtcctctnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaagcga
    14461 aacaccctgc ttgtgaaata aaatcagttt acgtccgagt ctccccaagg cggcgtgccc
    14521 tctacccgga gacccatact gcgagcagtg cctgccacca tgctcatggc agcatcaagg
    14581 ctgaagcagt taaggtcagc cattttgtct tcggcgatgg cttttacctg atcccaagtt
    14641 acagagccta ccttgttgcg attgggctgt gccgagccac tcttgatctt ggagacttcc
    14701 atcaattgag cagcggcagg agcggttttc agtacgaatt caaaggattt gtccttgaat
    14761 accgtaatga ttaccgggat gatcttaccc attttgtctt gcgtcacagc attgaagcgc
    14821 ttgcaaaact ccatgatatt cacaccttta gaaccgagcg caggaccgat tgggggcgca
    14881 gggttggctt ggccgccttt gacttgcagt tttataaagg tttcgatttc ctttgccatt
    14941 gttgtcgttt ttgccggcca gctcattgat tgctccgggg aagcgtattt cgccatgatt
    15001 acctgacgaa aacaggagcg caaaatttgc agaccaaatc tttattatgc ttgtttttct
    15061 acttgcatga aattgagttc cacctcagtg ccacggccaa aaattttcac aatgaccttc
    15121 agctttttct tttcctcgtt aacctcctga atatccccga caaactcgct gaacggccca
    15181 tcaataattt tgatggtttc gcccacgata aacggttcga tcagcgattc accagccccc
    15241 tgagattcat ctactttgcc caacatgcgg ttggcttcgg cttcacgcat gggaatcggc
    15301 gtgttcttgc ccagaaagtg gataacgttg ggcacattgg caatagcctg cacaatttcc
    15361 ggtgtaaacc gaaccggatc ggcctcaatg agcaaatacc ctggaagaat gttgcgctcc
    15421 gaaattactt ttttgccatt tcggatttta tatacctttt cagtgggtac aatcacctgc
    15481 gtaataaact cagaccagcc ctcgcgctgt atctccagct cgatacgctc tttgatcttg
    15541 cgttcttttc cgctgatcac ccgcaaagaa taccactttt tttcttccga catcgcttcg
    15601 ggaatatact taattggaac ctactccgta aatgagttta gtcaaagcat tggaaccgcc
    15661 gtccatcagg ataatgactc ccatcaaaat ggcagaagct accaatacgg ctactgtatt
    15721 ggacatcagg ctactcatag tgggccaggt cactttgtga accagttcat tatagctttc
    15781 cagcaagtac aatttgatct tttccatctc ggatatttat tgcacgggca gaaggacttg
    15841 aaccctcggc ctacggtttt ggagaccgtc gctctaccaa ctgagctatg cccgtatgac
    15901 aaataaatca gcagttaaca gagaaagatt aggcccccaa aagagtgcct aatctttgct
    15961 catatcactt ctgaaagaat cagatattaa tcaatgatct cagttacctg acctgcgcca
    16021 acggtacggc caccttcgcg aatagcgaaa cgaagacctt tttccatagc gatggtattg
    16081 aggagagata cttccatcgt aacgttatcg ccaggcatta ccatttctac gccttcggcc
    16141 agtttgcagt cgccggtaac gtcggtcgta cggaagtaga actgcggacg ataaccgttg
    16201 aagaacggag tgtgacgacc accttcttct ttgctcagga cgtaaacctc gcacttgaac
    16261 ttcttgtgcg gcttcacaga acccggagcg cagataacca taccgcgacg gatagcttct
    16321 ttttcaatac cgcggaggag caaacctgca ttgtcgccgg cttcaccacg gtcgagaatt
    16381 ttgcggaaca tttccacacc cgtaaccgta gaggtaagag gtttttcgcc ttctttcaac
    16441 atacctacga tttccacggg gtcaccggtt ttgataacgc cacgctcaat acgacccgta
    16501 gccaccgtac cacgaccggt gatagagaat acgtcttcta ccggcatgag gaaaggcttg
    16561 tcaacgagac gctcccgttg aggaatggca ttatcaacag catccatcaa ttctttgata
    16621 gctctttcgc catcggggtt accttcgaga gccttgaggg ctgaaccctt gatgatgctg
    16681 gcgctgtcgc catcgaagcc gtaggtgtcg agcaattcgc gcacttccat ttctacgagt
    16741 tcgagcattt cagggtcgtc cacaaggtca actttgttca taaaaacaac gatcttggga
    16801 acgcctacct gacgagccaa cagaatgtgc tcgcgggttt gcggcatcgg gccgtccgta
    16861 gctgctacta cgaggatggc gccgtccatt tgagcggcac ccgtaaccat gtttttcaca
    16921 tagtcggcgt gacccggaca gtcaacgtgt gcatagtgac ggttggccgt ctcatattcc
    16981 acgtgcgccg tattgatggt gatacctctt tctttctctt cgggagcggc gtcgatggaa
    17041 tcatagtcct tcttctctgc aagacccgac tgggaaagaa cgtaggtgat ggcagccgtg
    17101 agagtggtct taccatggtc tacgtgacct atcgtaccaa catttacgtg cggcttggtt
    17161 ctttgaaatt gctccttagc cattggtagt tgatttagga tatggtttaa aaagattaaa
    17221 gatgctgaac aagagccaat gaagggaatt gaacccccga ccccttcctt accatggaag
    17281 tgctctaccc ctgagctaca ttggcttaaa tgccataatg caacagaaaa taaagaagac
    17341 tgccggtgcg cctgcttcat tttttgagcg gacgacgaga ttcgaacccg cgaccctcag
    17401 cttggaaggc tgatgctcta ccaactgagc tacgtccgca ttgctaaaat ggtgggggta
    17461 ataggattcg aacctattca ggcatagcca acagatttac agtctgcccc gtctctccca
    17521 cttcggcgta cccccaaaag ataattatgt caaatcaaat acgctgcttt tgcaacattt
    17581 ctgcctattt tggctgagcc agtggaggga ctcgaacccc cgaccagctg attacaaatc
    17641 agctgctcta cca
//
LOCUS       DAORLF010000037        17606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1725_length_17606_cov_1.886331, whole genome shotgun sequence.
ACCESSION   DAORLF010000037 DAORLF010000000
VERSION     DAORLF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17606
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_17606_cov_1.886331"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    295..377
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            380..682
                     /locus_tag="PK198_05300"
     CDS             380..682
                     /locus_tag="PK198_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRF38178.1"
                     /translation="MELIKTQIGRLRILAFMEGVSFLVILFVTMPLKYLLQMPGPNYF
                     FGMAHGLLFVLYVVAVMLVKMEQDWGIKKTSLALLASVIPFGTFWADHKLFKPAAN"
     assembly_gap    727..812
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1101..1144
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1399..>3392
                     /locus_tag="PK198_05305"
     CDS             1399..>3392
                     /locus_tag="PK198_05305"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRF38179.1"
                     /translation="MAFDMQMLAAHYESFAQRVDQAKKALGRSLTLTEKILYAHLHPD
                     SSLQSYARGKDYVFFAPDRVAMQDATAQMALLQFMMCGRNQTAVPSTVHCDHLIQAKN
                     GSAADLKTANEINSEVYDFLASVSNKYGIGFWKPGAGIIHQIVLENYAFPGGMMIGTD
                     SHTPNAGGLGMVAIGVGGADAVDVMVGMPWELKMPKVIGVKLTGKLNGWTSAKDVILK
                     VAGILTVKGGTGAIVEYFGPGAEAMSCTGKGTVCNMGAEIGATTSIFGYDASMSRYLR
                     ATERADVAELADKVASYLTGDAECYANPGQYFDQVIEIDLDTLEPHINGPYTPDLAWP
                     ISKFAQAVRENNYPQRLEVGLIGSCTNSSYEDLDRAASVARQAVDKKLKVKAEFTITP
                     GSELIRYTVARDGQLEAFERIGGVVMANACGPCIGQWARHTDDPTRANSIITSFNRNF
                     SKRNDGNPQTRSFVASPEIVTAMAIAGDLCFNPLTDTLVNEDGQAVKLDPPSGVELPP
                     KGFDVEDAGYQAPAADGSGVNVSVSPTSNRLQLLTPFQPIASEQLKGMRVLLKAKGKC
                     TTDHISMAGPWLEFRGHLQNIARNTFISAINAFNGEANSVINYVENKYMAVPESGLAY
                     RDAGIGTVVFGEENYGEGSSREHAAMQPRFLGVKAVVVKS"
     assembly_gap    3393..3516
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3756..3861
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3862..4512
                     /locus_tag="PK198_05310"
     CDS             <3862..4512
                     /locus_tag="PK198_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HRF38180.1"
                     /translation="RLGELQRVMYAEKKHSVLVVLQGMDGSGKDGAVVNVFEDCAPMG
                     LQVVGFKKPTEEEFAHDFLWRIHKHAPQRGMINIFVRSHYEDILIQRVHKWISEERVE
                     KRMQSINALEELLAYDNHTQVCKFFLHISKEQQQIELQQRIDDPTKYWKHNSGDWRER
                     ELWPEYMRCYEDALNRSSIPWTIVPVDERWYRDYIIAKTLVEHLEKLDMKYPPLEK"
     gene            complement(4521..>4936)
                     /locus_tag="PK198_05315"
     CDS             complement(4521..>4936)
                     /locus_tag="PK198_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38181.1"
                     /translation="DLRTQQTGGGNKVKEEDFLSLFENFSLNHNLVMGWRTDNAGNAV
                     FDIATNAINVQGNIQLSPNWAINVGNFGYDFRQRGLSFPSFGFSRDLHCWSLLFNWQP
                     TRGTYNFSLAVKPGTLDFIKIPNQRNVADPLRAFE"
     assembly_gap    4937..4954
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4955..>6213)
                     /locus_tag="PK198_05320"
     CDS             complement(<4955..>6213)
                     /locus_tag="PK198_05320"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRF38182.1"
                     /translation="GLSWRHNQDRAAHPTRTFGGSVNIQTNNNQQRRYNDAQSVLNTQ
                     LNSNLSYNKTYPGKPYSLSVSMNHSQNAVSREMTINFPNIQFPVQALYPFKRKERVGA
                     ERWYESIVLRYSGEAANRFVTTDTTLFTRATLDNAQFGVRHTASTGTSFKVLKFFNLN
                     PGINYNEVWYLQSTQRNFIPGLETRDTIIQGVLRTDTLRLGRIEQFRRSGFTPYRTYS
                     ASLSLTTQIFGTMRFKSGYLRGLRHVIKPSISFNYSPNYNNPNLGYFQSVQDSIRADL
                     IRTYSVFEGGIYGGPPQSERQMALSYSFNNIFEAKLRGKKDTVDRKIKLFDNLVLGGS
                     YNFAADSLKFSPVTAAGTARFFKGMTTMSLGAAFDPYIADEQGRRINISVWQDRKQLL
                     RFTRADISFNTSITVGKLRSLLQGKPE"
     assembly_gap    6214..6229
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6230..6605)
                     /locus_tag="PK198_05325"
     CDS             complement(<6230..6605)
                     /locus_tag="PK198_05325"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRF38183.1"
                     /translation="MKIIPDKMVIVGPSYLEIGGVPTPIALPFGFFPIPGGKQTGLLF
                     PNDYEYSDQWGFGLRGVGWYFPINDHLNLQLRGNIYPFSGTWGVSATADYRKRYKYSG
                     GMTINYDSQRRENNEGTFDTINS"
     assembly_gap    6613..6636
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6637..7255)
                     /locus_tag="PK198_05330"
     CDS             complement(<6637..7255)
                     /locus_tag="PK198_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38184.1"
                     /translation="MKTKKYCIFFILLCGAWNLFAQQRPLPDSIPPAPADTLTFSTDS
                     LNLALDSLPPPLFSGVVFSKDSLDAPVEYEARDSMILDVEDQKVHLFGQAVVKYTTIT
                     LQADYIVFDWKSSIVSAEGRPDSTGQLAGFPQFSEGEQTFEAKRMRYNFKSRKGMVYE
                     VTSQQNDIFIHAEKSKFISGAVTETDTTANDVICSHNGLFTTCTAD"
     gene            7469..>8467
                     /locus_tag="PK198_05335"
     CDS             7469..>8467
                     /locus_tag="PK198_05335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF38185.1"
                     /translation="MPNRMRTVVIDAGHGGHDPGCLGSDRREKHITLSVAKRLAAAIQ
                     AAHPDLKVVLTRDTDVFVPLHERASIANRLHADLFISIHCNFMPGKSSVQGSETYVMG
                     LHTTEHNLAVAKRENAAILLEDNYETNYDFDPNSPEGHIALSMFQSAYIEQSILFADL
                     VEKHIALIGRKSRGVRQAGFVVLKATAMPSALIEIGFLSNKDEETYLKADDGQAAVTG
                     AILDAFSQYRILRESGTAALPVATTPKTVPAPQTNAQPSARSKPEGIPLGAIEQPAVA
                     ATPEPVSPPPASYSWPSTDTPKVIGQKTPPTDTRPRGTDGAAAKTEPAAVQVEFCVQ"
     assembly_gap    8468..8492
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8493..8714
                     /locus_tag="PK198_05340"
     CDS             <8493..8714
                     /locus_tag="PK198_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38186.1"
                     /translation="ADLNLPVWKSTGYQVQELKEDGRYKYLIRNITTYAQAQQVKNQV
                     QSNGFPGAFITAYQQGRRISVEEAIRLTK"
     gene            8783..>9051
                     /locus_tag="PK198_05345"
     CDS             8783..>9051
                     /locus_tag="PK198_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF38187.1"
                     /translation="MKISSEVKIGLLTIVTLAVSLWGFKFIQGKNMLRASNVYYVEYP
                     SIDLLKKATDVTINGFPVGVVSDMYLKPNDPEKRIVVVLDLKRDIL"
     assembly_gap    9052..9137
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9138..9841
                     /locus_tag="PK198_05350"
     CDS             <9138..9841
                     /locus_tag="PK198_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38188.1"
                     /translation="TGPDCAQSGDYLKGELQGFMSYMLGGDGTMDAYMASIKSGFADL
                     IDTLNQQLLSPESQTPIAESVADLRKSLANLESATAQLDQLMRRSGGKIDGTLGNLQS
                     ITGNFDSKEKEINTLIDNFSAVSTQMKEADLKKTLEEVKASVSNLKTTLATTEQTLGS
                     VNTMMGKINAGEGSLGKLLKDDALFNDLSGLSTKADSLMTDIQTRPYRYMPLKSRARV
                     KKFDRLDAKETGTKQ"
     gene            9813..>10153
                     /locus_tag="PK198_05355"
     CDS             9813..>10153
                     /locus_tag="PK198_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38189.1"
                     /translation="MRKRPVPSNDAAVRNRYAELGVEVYYREEGSDYNNPHFPQIRQL
                     LLNNRHRIDFSHSLDFCCGSGEVSKVVLEMGFPLPSASDPFTAEAYGRNFDAECLPWS
                     FDDVIRGKAEGR"
     assembly_gap    10154..10227
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10572..11060
                     /locus_tag="PK198_05360"
     CDS             10572..11060
                     /locus_tag="PK198_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38190.1"
                     /translation="MEQVVQEGIGDADFIVAASAWRSGDFVHTPPLKRGYKPVLAYPI
                     LSKSQMDSIDKGMAVHPAIIAWTQRFDANCVNAGNCVPKEEFTLKGITRKIDKERNRV
                     TTLPKDRYIIPENITYLETEREPLAWYWNLVIMLAALALGGGTEWYHSRKSKNTTEPT
                     QK"
     gene            11067..>11813
                     /gene="tpiA"
                     /locus_tag="PK198_05365"
     CDS             11067..>11813
                     /gene="tpiA"
                     /locus_tag="PK198_05365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRF38191.1"
                     /translation="MIQRQRIVAGNWKMNKSYVEGRELVKEISDRLQPTDTMVILAPP
                     FIHLNNVGNNIRGVANMFLAAQNCHWAESGAYTGEISARMLESIEVQYVIIGHSERRE
                     YFLETDEILAKKIDAVLAHNMRPIFCCGEKLEVREAGTQEQLVEEQLRKALFHLSPEA
                     FQKVVIAYEPVWAIGTGKTASPQQAQDMHAFIRSVIAGQYGDEAAQATSILYGGSCNP
                     SNARELFGQADVDGGLIGGASLKADDFLAIV"
     assembly_gap    11814..11920
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11921..12174
                     /locus_tag="PK198_05370"
     CDS             <11921..12174
                     /locus_tag="PK198_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38192.1"
                     /translation="FTILSFLLMIAAIPRSIKVSERHPMIIIGLLSGLIFLFFLVGVI
                     SSIIALVRREPSHWTNWVGSIANLLYLLAVIATLLFTKD"
     gene            complement(12240..12311)
                     /locus_tag="PK198_05375"
     tRNA            complement(12240..12311)
                     /locus_tag="PK198_05375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12276..12278),aa:Arg,seq:cct)
     gene            12392..12757
                     /locus_tag="PK198_05380"
     CDS             12392..12757
                     /locus_tag="PK198_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRF38193.1"
                     /translation="MNIEEFRDYCMAKKAVEETFPFDEVTLVFKVMGKMFAITGLDNE
                     RFTVTMKCDPDWAEELREEHPEIQPGYHTSKRLWNTVDFEGMLDDAFLCRLIDHSYDQ
                     VLAGLPKKVRQEWEQWPES"
     gene            12754..>13162
                     /locus_tag="PK198_05385"
     CDS             12754..>13162
                     /locus_tag="PK198_05385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF38194.1"
                     /translation="MTDFLIIGQGLAGTLLAHFLQSDGYTVRIVDEYQVRASTRVAAG
                     LINPVTGRRFVKSWRIDDLLPFARQTYRLLESQLGVALYHERPIYRALYSVEEENLWL
                     ERCADLQYRQYVSDEGLPESIAPYIHPPRGFGRV"
     assembly_gap    13163..13251
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13252..>13600
                     /locus_tag="PK198_05390"
     CDS             <13252..>13600
                     /locus_tag="PK198_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRF38195.1"
                     /translation="AAPFDFAQLQISEDAVRYGSLSARHIVFCEGRWGKTNPLFSELP
                     LVGDKGEVLLVRLPAGVSLEAIIKHRVFVVPMGEDLYWVGSNYSSRYESDAPTEQGRG
                     WLEENLRRSLRCSF"
     assembly_gap    13601..13613
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13614..13822
                     /locus_tag="PK198_05395"
     CDS             <13614..13822
                     /locus_tag="PK198_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF38196.1"
                     /translation="LDHRAAVRPTVKDRRPLLGRHSEYGNVLIFNGLGTKGTSLGPFW
                     ARHFADFLGGKTELDAEVDVRRFG"
     gene            13917..>14109
                     /locus_tag="PK198_05400"
     CDS             13917..>14109
                     /locus_tag="PK198_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRF38197.1"
                     /translation="MPTFSIDLKEGKLVKNSDLIVGIDLGTTNSLVAYMKDGAAVAVK
                     DPDGKSALVPSIVHFLPEGG"
     assembly_gap    14110..14129
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14130..15791
                     /gene="hscA"
                     /locus_tag="PK198_05405"
     CDS             <14130..15791
                     /gene="hscA"
                     /locus_tag="PK198_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRF38198.1"
                     /translation="LVGDEAKEKLVSDPRHTIYSVKRLMGKSYRDVKGFEQYFGYQII
                     DEDTESLVKIRVKDRFYTPIELSAEILKALKARIEGVLEQPVSKAVITVPAYFNDAQR
                     QATRDAGKLAGLDVLRIVNEPTAASLAYGIGLHPDDNQVIAVYDLGGGTFDISILRIE
                     QGVFEVLSTNGDTFLGGDDFDRVIMDFWADQNADLRSLLETDKGLRQEMRLLAESAKK
                     QLSSSDWFEGRVGERHLEISAQQFETLIQPFVDRTIDACRSALRDAGLQASDIQHVIM
                     VGGSTRTPLVKRSVAAFFGREVNDSLNPDEVVALGAAIQADVLAGNQKDLLLIDITPL
                     SLGIETMGGLMDVILPRNSKVPARVGRSYTTSVDGQRNLKVAVYQGERDLVAHNRKLG
                     EFILRGIPPMPAGLPKIEIHFILDADGILRVRAKELRSGVEQEVEMRSQYGISEEEMA
                     LMLIDSIQNAQSDMAARALLEARNEARSVLLATDRFLIQNAELFSPEEKAQITAIAGE
                     LDAAVQGEDKDLINKVLEDLNTYSAPLAHRALEANVAQALKGNKV"
     gene            complement(15901..16728)
                     /locus_tag="PK198_05410"
     CDS             complement(15901..16728)
                     /locus_tag="PK198_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRF38199.1"
                     /translation="MHNSMIFMAITALLLSNCRPQSPQTDLALSAAAETAALTETTAP
                     EAETTTRQDSFTIAYLTGQFDPASHPDFTTIDIKYADRTGQYLRRDAYEAFKKMHAAA
                     AADGVTLRIISATRNFNRQKEIWEAKWNGSRLVDGKNLAETMPDPVQRALKILEYSSM
                     PGSSRHHWGTDIDINALTDGYFRQGEGLKIFEWLTAHAHEYGFCRPYSAKGPERPNGY
                     FEEKWHWSYQPVSGQLTELARTQLKDSMIRGFAGAETAGSIGVVQKYVLGVSKECTR"
     gene            complement(16824..>17303)
                     /locus_tag="PK198_05415"
     CDS             complement(16824..>17303)
                     /locus_tag="PK198_05415"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF38200.1"
                     /translation="KIEDILANISKYSPAVRNNGGGFYNHNLFWEIMSPNGGGEPTPQ
                     MNIHKAIERDFGSFENFKTQFTNAAMTRFGSGWAWLCVDGNDHLFVTSTANQDNPLMD
                     IHDAKGTPILGLDVWEHAYYLNYQNRRPDYVAAFFNVINWHEVTKRFNDANPGRSEF"
     assembly_gap    17304..17325
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17326..17447)
                     /locus_tag="PK198_05420"
     CDS             complement(<17326..17447)
                     /locus_tag="PK198_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF38201.1"
                     /translation="MAFQLPELPYAYDALEPHIDARTMEIHHTKHHAAYTNNLNA"
BASE COUNT         3886 a         4335 c         4586 g         3862 t
ORIGIN      
        1 ctttaggact tgaatacaag aatttgcaca agtatgggac actgttgtgc gcaacacaac
       61 atgcggaatg agcatctcga aaatgtggga aggaggaaac ggctgagtag gttgcaggcg
      121 cactaaaatt gtcatgtttt aggtgtgata ccgtcaatca cagtattgac ggtgcaaaga
      181 tatagtgtct ctttgagaat gtatttaaaa aaatatcaag cattagcaag tgtctgttta
      241 agaggttttt gagaacgatt tacaagttag atcagccgtc tgtaatcaaa cattnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnaaa tggaattgat aaaaacacaa attgggcgct tgcggatact
      421 ggcttttatg gagggcgtgt cgtttttagt catccttttt gtgacgatgc cgctcaaata
      481 tctcttacaa atgcccggcc ccaattactt tttcggaatg gcgcatggct tgctgtttgt
      541 gctgtatgtg gtggcggtga tgctggtgaa aatggagcag gattggggaa tcaaaaaaac
      601 ctctctggcg ctgctggcct cggtgatacc tttcggcact ttctgggccg atcataaatt
      661 gttcaaacct gctgccaatt gatacgcaaa acagactgct ctaaattgaa acgtcccgaa
      721 aacagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatcaagt cagcagttgg cggctcgcag
      841 ctttttatcg cttgcccatt ttcgccgtca gctcctcata cagggttctg atttcgccgc
      901 ttgcgcccat catgcagccc gaaacagaga tgcccgaaac cttgaacccg aaaccttact
      961 tgtactcctc ccgcaccggg caaaacacat ccagaatttt gcagggcgtc agcgcgcggc
     1021 cactatgctc tacatcggag ggaatgaccg ccaccaagcc gggttcatac acctgagtct
     1081 cgccggcaac agtgagttca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnggcagt tcggcgccgg cctctatttc ccaaaaaacc agcgtggact tttcggtgtg
     1201 aacaaagcgg ccccggaagc ctttaatgag ttctttcggt tctatttgaa gcaggtcgta
     1261 agtcatggtt cttattttga aagcaaagat gcagacttac ccgatatttc tgtttcaatg
     1321 gctcgttttt tgaggcaaag cagtatattt gcgcgccttt aaaacaacat ttcttaccat
     1381 taaaatacca gcaccactat ggcattcgat atgcagatgc tcgcggctca ttacgagtct
     1441 ttcgctcaac gggttgatca ggccaaaaaa gcattgggcc gctccctgac tctgaccgaa
     1501 aaaatccttt acgcacactt gcaccccgat tcctcgctgc aatcttatgc gcgtggcaaa
     1561 gactatgtgt ttttcgctcc cgaccgggtg gccatgcaag atgctacggc tcaaatggcg
     1621 ctccttcagt ttatgatgtg cggccgcaac caaaccgccg tgccctccac cgtacactgc
     1681 gaccacctta tacaggccaa aaacggttcg gctgccgacc tcaaaactgc caatgaaatc
     1741 aactcggaag tgtatgattt tctggcctct gtatccaaca aatatggcat cggcttctgg
     1801 aaaccgggcg cgggcatcat ccaccagatc gtactggaaa actatgcttt ccccggcggt
     1861 atgatgatcg gcaccgactc acataccccc aacgcgggcg gcctcggtat ggtggccatc
     1921 ggcgtgggcg gcgctgatgc cgtggacgtc atggtaggaa tgccctggga actgaaaatg
     1981 cccaaagtga taggcgtgaa gctgaccggc aaactcaatg gctggacttc ggccaaagat
     2041 gtgatcctaa aagtagccgg tatcctcacc gtgaaaggcg gtacgggcgc tattgtagag
     2101 tatttcggcc cgggtgcaga ggccatgtct tgtaccggca aaggcacggt ttgcaacatg
     2161 ggcgctgaga tcggagctac cacctctatt tttggttacg acgcttccat gagccgctac
     2221 ctgcgcgcca ccgaacgcgc tgatgtggcg gaattggccg ataaggtggc ctcctacctc
     2281 accggcgatg cggaatgtta tgccaatccg gggcagtatt tcgatcaggt cattgaaatt
     2341 gatttggata cgctggagcc gcacatcaac ggcccttata cccccgacct cgcctggccg
     2401 atctccaaat ttgctcaggc cgtgcgcgaa aacaactacc cgcagcgcct cgaagtgggt
     2461 ctcatcgggt cttgtaccaa ctcttcttac gaagacctcg atcgcgctgc ttccgttgcc
     2521 cggcaggcag tggacaaaaa gctgaaagtg aaagcggaat ttaccatcac gccgggttcc
     2581 gaactcatcc gctacaccgt agcacgcgac ggccagctcg aagccttcga acgcatcggc
     2641 ggcgtggtga tggccaatgc ctgcggcccc tgcatcggcc aatgggcgcg ccacacggat
     2701 gatcctaccc gtgccaattc catcatcact tccttcaacc gcaacttctc caagcgcaac
     2761 gacggcaacc cccaaacgcg ctctttcgtg gcctcgcccg aaatcgtgac ggctatggcc
     2821 attgccggcg acctttgctt caatccgctg accgacacgc tcgtcaatga agacgggcag
     2881 gccgtgaaac tcgatccgcc cagcggcgta gaattgccgc ccaaaggctt cgatgtggaa
     2941 gatgccggct atcaggcgcc cgccgccgac ggcagcggcg tcaatgtcag tgtttcaccc
     3001 acgtccaatc gccttcaatt gctcacgccc ttccagccca tcgcttcgga gcaattgaag
     3061 ggcatgcgcg ttctcctcaa ggcgaaaggc aagtgtacga ccgaccacat ttcgatggcc
     3121 ggcccctggc tggagttccg cggtcacctg caaaacatag cccgcaatac attcatcagc
     3181 gccatcaatg ctttcaacgg cgaggccaac agcgtcatca actacgttga gaacaaatac
     3241 atggctgtac ccgaatccgg tcttgcttat cgcgacgcag gcatcggtac ggtggtgttt
     3301 ggtgaagaaa attacggcga aggctcttcc cgcgagcacg ccgccatgca gccccgcttc
     3361 ctcggagtga aggccgtggt ggtcaaatca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatg gcgcccggtc gcaatctgac
     3541 catgatactc caccacgccg acggtacgca ggacagcata gaactgagcc atacctacaa
     3601 tcaggctcag attgaatggg tgcagtcggg ttcggcgctc aacaaaatca gaaaaggatt
     3661 aggagcataa aagcactcct gatgtggtga tgaagtgagg ggttgcatat agctgctccc
     3721 acttcatcgc acggtctttt ctacctgttt tttctnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn ncgcttgggc gaactccagc gggtgatgta tgctgaaaaa
     3901 aaacactctg ttttggtggt gttgcagggt atggacggca gcggcaaaga cggcgcggtg
     3961 gtcaatgttt ttgaggattg cgctccgatg ggcttgcagg tggtgggttt caaaaagcct
     4021 accgaggagg agtttgcaca cgattttctc tggcggattc acaaacacgc cccccaacgc
     4081 ggcatgatca atatcttcgt tcgctctcat tatgaagaca ttctgattca acgcgtacac
     4141 aaatggatca gcgaagagcg agtggaaaaa cggatgcagt ccatcaatgc tcttgaggaa
     4201 ttgttggcgt atgacaacca tacgcaggtg tgcaaattct ttctgcacat ttccaaagag
     4261 caacagcaaa ttgaactcca gcaacgcata gacgacccca cgaaatactg gaaacacaac
     4321 tccggcgatt ggcgcgaacg cgaactctgg cccgaataca tgcgctgcta tgaggatgcg
     4381 ctcaatcgtt cttccattcc ctggacgatt gtgccggtgg acgagcgctg gtatcgcgat
     4441 tacatcattg ccaaaacgct ggtagagcat ctggaaaaat tggatatgaa gtatccgcct
     4501 ttggagaagt aaaagcccct ttactcgaac gcccgcaagg ggtcggccac gttacgctgg
     4561 ttcgggattt tgataaaatc cagggtgccg ggtttgacgg cgagggaaaa attataagta
     4621 ccccgcgtag gttgccagtt gaacaacaaa ctccaacaat gcaaatctct gctgaaacca
     4681 aaggacggaa acgacaaccc tctttgcctg aaatcgtagc cgaaattgcc cacattgatg
     4741 gcccagttgg ggctgagttg tatattgccc tgcacattga tggcattcgt ggcgatgtcg
     4801 aaaacagcat tgcccgcgtt gtcggtgcgc cagcccatga cgaggttgtg attgaggctg
     4861 aagttttcaa acaaactgag gaaatcctct tctttcactt tgttgccgcc cccggtctgc
     4921 tgcgttcgca ggtcttnnnn nnnnnnnnnn nnnntcttcc ggtttgcctt gcaatagaga
     4981 cctcagcttg cccaccgtga tactggtatt gaaactgatg tctgcgcggg tgaagcggag
     5041 cagttgtttg cggtcttgcc agactgaaat gttgattcta cggccctgct catcagcgat
     5101 gtaaggatcg aaagccgctc cgaggctcat ggtggtcatg cctttgaaaa aacgggcagt
     5161 acctgcggca gtcaccgggc tgaatttgag agaatcggca gcaaaattgt aggaaccgcc
     5221 cagtaccagg ttgtcgaaga gtttgatttt gcggtccacc gtatcctttt tacctcgcag
     5281 tttggcctcg aaaatattgt tgaacgaata cgacagcgcc atttgccgtt cgctttgcgg
     5341 cggccctcca taaatgcccc cctcgaaaac ggagtaggtt cggatgagat cggcgcggat
     5401 agaatcctgc acactttgga aataccccag attgggattg ttgtaattgg gcgagtaatt
     5461 gaacgaaata ctgggtttga ttacatgccg caagccgcgc aggtagccgc ttttgaaacg
     5521 catggtgccg aatatctgcg tagtgagcga gaggctggcc gaataagtgc ggtacggtgt
     5581 gaagcccgat cttcggaatt gctcgatgcg ccccagacgg agggtatcgg tgcgcagaac
     5641 gccctgaatg atggtgtcgc gggtttcgag tccggggatg aaattgcgct gcgtagattg
     5701 taggtaccac acttcgttgt agttgatgcc ggggttgaga ttgaaaaact tcagcacctt
     5761 gaacgaggtg ccggtactgg ccgtgtgccg cacgccgaac tgtgcgttgt ccaaagtggc
     5821 gcgggtaaac agcgtggtat cggtagtaac gaaccggttg gctgcctcac ccgaatagcg
     5881 cagcacgatg ctctcgtacc agcgttccgc cccgacgcgt tccttgcgtt tgaaaggata
     5941 gagcgcctgc accggaaact ggatattggg gaagttgatg gtcatttcgc ggctgacggc
     6001 gttctggctg tggttcatgc tcacgctgag gctgtacggt ttgccggggt aggttttgtt
     6061 gtaagacagg ttggagttga gttgtgtgtt gagtacgctt tgggcatcgt tgtagcggcg
     6121 ttgttggttg ttgttggttt gaatattgac gctgcccccg aaagtacggg taggatgcgc
     6181 cgcgcgatcc tgattgtgcc gccaggagag tccnnnnnnn nnnnnnnnna agaattgatg
     6241 gtatcaaaag tgccttcatt gttttctcta cgttgagaat catagttgat ggtcatgccg
     6301 ccggaatact tgtagcgctt gcggtagtcg gcagtagccg aaacgcccca ggtgccggaa
     6361 aatgggtaga tgttgcccct caactgcagg ttgagatgat cattgatggg gaaataccag
     6421 cccacgccac gcaagccaaa gccccattgg tcggaatact cgtaatcgtt gggaaaaagc
     6481 agaccggttt gtttgcctcc gggaatggga aagaagccaa acggcagtgc aatgggagtg
     6541 ggtacgccgc cgatttcgag gtaagatggc cccacgatga ccatcttatc tgggatgatt
     6601 ttcatttttt tgnnnnnnnn nnnnnnnnnn nnnnnnggtc ggccgtacag gtggtgaaca
     6661 ggccgttgtg gctgcaaatc acatcgttgg cggtggtatc ggtttccgtg actgcgccgc
     6721 tgatgaattt ggatttttca gcgtggataa agatgtcgtt ttgctgcgaa gtgacctcat
     6781 acaccatgcc cttgcgcgat ttgaaattgt agcgcatgcg tttggcctcg aaggtttgct
     6841 cgccttctga aaattgggga aaacccgcca attggccggt gctgtcgggg cgcccttcgg
     6901 cagagacgat gctcgatttc cagtcgaaaa caatataatc ggcttgcagc gttatggtgg
     6961 tgtatttcac aacagcctgc ccgaagagat gcactttttg gtcttccacg tccaaaatca
     7021 tagaatcgcg ggcctcatac tctaccggtg cgtccaaaga atctttggaa aataccacgc
     7081 cactgaacag agggggaggc agggagtcga gggcgaggtt cagggagtcg gtgctgaagg
     7141 tgagcgtatc ggcaggagcc ggcgggatgc tgtcgggcaa agggcgctgc tgggcgaaga
     7201 ggttccacgc gccgcacagc aagatgaaaa atatgcagta cttctttgtt ttcaagcgat
     7261 tataaactta ttttgcacga caaaaccaac tacgctgttg attcaggaaa actttacaat
     7321 gagaaacatt caaaggcaac ccacgtccca aacgcgccaa tgccatcttc cgtccctgct
     7381 cttaaaaggc tttaacgttt tcttagcgtt cgcgctgtgg gccattcccg cctcaatcgg
     7441 gcgcgaaatt atcaaacccg ccgatatttt gcccaacaga atgcgcaccg ttgtcattga
     7501 cgcggggcat ggcggccacg atccgggctg tctgggcagc gacaggcgcg aaaaacacat
     7561 caccctctcg gtggccaaac gcctcgccgc agctattcag gccgctcacc ccgatctcaa
     7621 agtggtactc acccgcgata ccgatgtgtt cgttcctttg catgagcggg cctccatcgc
     7681 caatcgcctg cacgccgacc tgttcatctc cattcactgc aattttatgc caggaaaatc
     7741 ctcggtacaa ggttccgaaa cctatgtgat gggcctgcac accaccgagc acaacctcgc
     7801 tgtggcaaaa cgagaaaatg ccgctatctt gttggaagat aactacgaaa cgaattacga
     7861 tttcgacccc aactcgccgg aaggccacat tgcgctcagt atgtttcaga gtgcctacat
     7921 cgagcagagc atcctgtttg ccgacctcgt agagaagcac atagcgctca tcgggcgcaa
     7981 aagccggggt gtgcggcagg ccggttttgt ggtactcaaa gctaccgcca tgccctcggc
     8041 cctcatcgaa atcggttttt tgagcaacaa agatgaggaa acgtacctca aagcggatga
     8101 cggccaggct gccgtcaccg gggccatcct tgatgcgttt tcgcaatatc gcattttgcg
     8161 cgaaagcgga accgctgcat tgcctgtggc aacgactccc aaaacggtgc ctgcgcccca
     8221 aacaaacgct cagccgtccg cccgctccaa accggaaggc atcccgttgg gcgccataga
     8281 gcaacccgcc gttgccgcca cgcccgaacc cgtatctccg ccgccggctt cttactcctg
     8341 gccttctacc gatacgccca aagtgatagg ccaaaaaacg ccgccaacag acacccggcc
     8401 acgcggtaca gacggggctg ccgccaaaac ggaacccgct gcggttcagg tggagttttg
     8461 tgtgcagnnn nnnnnnnnnn nnnnnnnnnn nngccgattt gaatttgccg gtttggaaaa
     8521 gcacgggcta tcaggtgcaa gagttgaaag aagacggcag gtacaaatat ctgatcagaa
     8581 acataacaac ctatgcacag gcacaacagg ttaaaaatca ggtacaaagc aacggctttc
     8641 cgggcgcttt catcacggct taccaacagg gccgccgcat cagcgtggaa gaggccatcc
     8701 gattgaccaa atgattcaaa aaccggcgtg ttgaatggag ttttgctacc tttgtgcgct
     8761 caatgatcca aaatcctttt caatgaaaat ttcaagtgaa gtaaaaatcg gcctgctcac
     8821 tattgtcacc ctggcggtct cgctttgggg gttcaaattc attcagggca agaatatgct
     8881 gcgcgcttcc aatgtatatt atgtggagta ccccagcatt gacctgctca aaaaggctac
     8941 cgatgtgacg atcaacggct tccccgtagg cgttgtgtcc gatatgtatc tcaaacccaa
     9001 cgaccccgaa aaacgcattg tggtggtgct tgacctcaaa cgagacattc tnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnngta ccggccccga ttgcgcccaa agcggcgatt atctcaaagg
     9181 cgaattgcag ggttttatgt cttatatgtt gggcggcgac ggcactatgg acgcatacat
     9241 ggcctctatt aaatcgggtt ttgccgacct tattgatacg ctcaatcagc agcttctcag
     9301 ccccgaaagc caaacgccga ttgcagaaag tgtggccgac ttgcgcaaat cgctggccaa
     9361 cttagagtcg gctaccgccc aacttgatca actcatgcgt cgctccggcg gcaagattga
     9421 cggcacgctg ggcaatttgc agtccattac tgggaatttt gactctaaag aaaaggaaat
     9481 caacacttta attgataatt tcagcgcggt gagcactcaa atgaaagaag ccgatctgaa
     9541 aaagacgctc gaggaagtaa aggcttcggt gagcaatctc aaaaccacgc tggctactac
     9601 ggagcaaaca ttgggcagcg tcaacacgat gatgggcaaa atcaatgcgg gcgaaggctc
     9661 gctgggcaag ctgctcaaag acgacgcttt gttcaacgat ctctccgggc tgagtaccaa
     9721 agccgactcg ctgatgaccg atattcagac gcgcccgtac aggtacatgc cgctgaaaag
     9781 ccgtgccaga gttaaaaaat ttgatcgctt ggatgcgaaa gagaccggta ccaagcaatg
     9841 atgcggctgt tcgcaaccga tatgccgaac tgggcgtaga agtctattac cgcgaggaag
     9901 gcagcgatta caacaacccg cattttccgc agatcaggca gttgttgttg aataaccggc
     9961 atcggataga cttcagccac agcctcgatt tttgttgcgg cagcggcgaa gtcagcaagg
    10021 tggtactcga aatgggcttc ccgctcccgt ctgcttccga cccattcaca gcagaggcgt
    10081 acgggcgcaa tttcgatgcg gaatgcctcc cctggagctt cgacgacgtg atccggggca
    10141 aagccgaggg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnctc acgcgccaac tgttgtctat ggcgccgcaa
    10261 ttggtagtcg ttactccgca caagcggccc gacttatcca agctcgaagg ggtgcaactg
    10321 gatttcgagg atgcttggcg taccgagcgc ggcaaatcgg tatttttgaa atcgtattct
    10381 ttgcggtagt ccggtattca gtaggtggtg ggcttttccc aacccggaac ctggaacccg
    10441 gaacccggaa ttgtgaatct gaaataatca accatgcttt tcagaattgt catcgttctt
    10501 ttcagtgtag tcgctttcat caatggatgc aacagcctga tttcccgcgt agccggcacc
    10561 gccaatacag tatggaacaa gtggtacaag agggcatcgg cgacgccgat tttatagtag
    10621 ctgcctcggc ctggcgcagc ggcgactttg tacatacgcc gcccctcaag cgcggctaca
    10681 agccggtatt ggcctacccg atattgagca agagccagat ggacagcata gacaaaggca
    10741 tggccgtgca tcctgccatc attgcatgga cacaacgctt cgatgcaaac tgtgtcaatg
    10801 caggcaattg cgttcccaaa gaggaattca ccctcaaggg cattacccgc aaaattgaca
    10861 aagaacgcaa ccgcgtgaca acgttgccca aagaccgcta catcatcccg gaaaacatca
    10921 cctacctcga aaccgaacgc gagcctttgg cctggtattg gaatttggtc atcatgctgg
    10981 cagcgctggc actgggcggc ggcacagaat ggtatcattc ccggaaatcg aaaaacacaa
    11041 cggaaccaac tcaaaaataa caacttatga tacagagaca acgcattgtg gccggcaact
    11101 ggaaaatgaa taagtcatat gtggaaggcc gcgagctggt aaaagaaatt tccgaccgcc
    11161 tgcaacccac cgatacgatg gtcatcctgg cgccgccctt tattcacctc aacaatgtgg
    11221 gcaacaacat tcgaggcgtg gccaatatgt tcctcgccgc ccaaaactgc cactgggcag
    11281 aaagcggcgc ctacaccggc gaaatctccg cccggatgct cgaatccatt gaggtgcaat
    11341 atgttatcat tgggcactcc gaacgccgcg agtacttcct cgaaaccgac gagatattgg
    11401 ctaaaaaaat agacgccgtt ttggcgcaca acatgcgtcc gattttctgc tgcggcgaaa
    11461 aactcgaagt gcgcgaagcc ggcacgcagg aacaattggt ggaggaacaa ctccgcaaag
    11521 ccctgttcca cctgtcgccg gaggcatttc aaaaggtcgt catcgcttac gaacccgtgt
    11581 gggccatcgg taccggcaaa acggcctcgc cccaacaggc acaagacatg cacgctttca
    11641 ttcgcagcgt cattgccggc caatatggcg atgaagcagc tcaggctacc tccattctgt
    11701 acggcggcag ttgcaatccc tccaatgcgc gcgagctgtt cggtcaggcc gatgtggacg
    11761 gggggcttat tgggggcgcc tcgctcaaag ccgatgattt tctggcgata gtgnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgttcacgat attgtctttt
    11941 ttgctgatga tagcagctat tcccagaagc attaaggtat cagaaaggca ccctatgatt
    12001 attatcggtt tattgtcggg gctgattttc ctttttttcc tcgtcggggt gatttcctcc
    12061 atcatcgcgc ttgtgaggag agagccttct cattggacca attgggtagg cagtattgcc
    12121 aacttgctgt accttctggc ggttattgcc acgcttcttt ttaccaaaga ctaaaagagg
    12181 tattgataaa tgtatgacgg taaatatttg attttcagtt gattatgagg atgggttagt
    12241 gatcccgcca ggaatcgaac ctggatctag agtttaggaa actcctattc tatccgttga
    12301 actacgggac cgacagcagg aaagtgcttt cggggcgcaa agttatgttt ttaaccggca
    12361 acctgaaaag aaagttttgt aattttcggc catgaatatt gaggaattcc gagactactg
    12421 catggccaaa aaggccgtag aagaaacctt cccttttgac gaggtgacgt tggtttttaa
    12481 ggtcatgggc aagatgtttg ccatcaccgg ccttgacaat gagcgcttta ccgtcaccat
    12541 gaaatgcgat cccgactggg ctgaagaact ccgcgaggag caccccgaaa ttcaacccgg
    12601 ctatcatacc agcaaacgcc tttggaatac cgtggatttt gagggcatgc tcgacgatgc
    12661 gtttttgtgc cgcctcattg accactccta cgatcaggtg ctggccgggc ttcccaaaaa
    12721 agtgcggcag gaatgggaac aatggcccga atcatgacgg attttctcat catcgggcag
    12781 gggctggcgg gtactttgct ggcgcatttt ctgcaatcag acggatatac ggtgcgcatc
    12841 gtggacgaat accaggtccg cgcctccacc cgcgtggccg ccgggctgat caatcccgtc
    12901 acgggccgcc gcttcgtaaa gtcttggcgc atagatgatt tgctcccttt tgcccgacag
    12961 acttaccgcc tgctggaatc ccaattgggc gtggcgctct accatgaacg gcccatttac
    13021 cgggccttgt actcggtgga ggaagaaaac ctctggttgg agcgctgtgc cgatctgcag
    13081 tatcgtcaat atgtatccga cgaggggctg cccgaaagca tcgcaccata cattcaccca
    13141 ccgcgcgggt tcgggcgggt aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcggctcct
    13261 tttgattttg ctcagttgca aatttcggaa gacgccgttc gatatggcag tctgagcgcc
    13321 cggcacatcg ttttctgcga agggcgctgg ggcaaaacca accccttgtt cagcgagttg
    13381 ccgttggtgg gcgacaaagg cgaggtactc ttggtacgct tgcctgccgg agtgtcgctg
    13441 gaggccatca tcaagcacag ggtttttgtg gtgccgatgg gcgaggactt gtactgggtg
    13501 gggtccaatt acagttcgcg ttatgagtcg gatgcgccca cagagcaggg taggggatgg
    13561 ttagaggaaa atctgcggcg ttcgcttcgt tgctctttcg nnnnnnnnnn nnntgttgga
    13621 ccaccgggct gcagtgcggc ctaccgtgaa agatcgccgt cccttgctgg gcaggcattc
    13681 cgaatacggc aatgtgctta ttttcaacgg attgggtaca aaaggcactt cgctggggcc
    13741 gttttgggca cgtcattttg ccgatttcct tgggggtaaa accgagctgg atgcggaggt
    13801 ggatgttcgt aggtttgggt gaaagcccaa atcacgcttc cccggcattt ctctgaaccc
    13861 cttaccgccc aaactgttac ctttgcgttt ttctgtacaa catccgattt tgcatcatgc
    13921 ccactttttc catagacctg aaagaaggta aactcgtaaa aaacagcgat ctcatcgttg
    13981 gtattgacct cggcactacc aacagccttg tggcgtatat gaaagacggc gcggcagtgg
    14041 ccgtcaaaga ccccgacggc aaaagcgcgt tggtgccctc catcgtccat tttttgcctg
    14101 aaggcggcgn nnnnnnnnnn nnnnnnnnnc tggtgggcga cgaggccaaa gaaaagctgg
    14161 tttccgaccc acgccatacc atttactccg tgaagcgcct catgggcaag tcttaccgcg
    14221 atgtgaaggg ttttgagcaa tatttcggct accagatcat tgatgaagac acggaaagcc
    14281 ttgtaaaaat aagggtaaaa gatcggtttt acacgcccat cgagttgtct gccgaaatcc
    14341 taaaagccct caaagcccgc attgaaggcg tactcgagca gccggtttcc aaagccgtca
    14401 ttaccgtgcc cgcctatttc aacgatgccc agcgacaggc cacccgcgat gccggaaaat
    14461 tggccggact tgatgtattg cgcattgtga acgaacccac tgccgccagt ctggcctatg
    14521 gtatcggctt gcatcccgat gacaatcagg tcattgcggt gtatgatctc ggcggcggca
    14581 ctttcgatat ttccattctc cgcattgagc agggtgtatt tgaggtactc tctaccaatg
    14641 gcgatacctt cctcggcggc gacgattttg accgcgtcat catggatttt tgggcagatc
    14701 agaatgccga tttacggagc cttctggaga ccgataaggg gctgcggcag gaaatgcgcc
    14761 tgctggccga gtccgccaaa aagcaactca gttcatccga ctggtttgag ggcagggtag
    14821 gggagcgcca cttagaaata tcagcacagc aattcgagac gctcatccag ccattcgtgg
    14881 atcgcaccat agacgcctgc cggagcgcgc tccgtgatgc gggcctccaa gcatctgata
    14941 ttcagcatgt catcatggtg ggaggttcta cgcgcacgcc gctggtgaag cggtcggtgg
    15001 ctgctttttt cggtcgcgag gtgaacgatt cgctcaatcc cgacgaggta gtagccctgg
    15061 gggccgccat tcaggccgat gtgctggcgg gcaaccaaaa agacctgctg ctcatagaca
    15121 tcacgccgct gtcgctgggc attgagacca tgggcggcct catggatgtg attttgccgc
    15181 gcaactcgaa agtgccggcg cgtgtgggcc gcagttacac cacctcggtg gacgggcagc
    15241 gcaacctcaa agtggcggtg tatcagggcg agcgggattt ggtggcgcac aatcgcaagc
    15301 tcggcgagtt catcctgcgc ggcataccgc ccatgccggc gggtttgccc aaaattgaaa
    15361 tccattttat tttggatgcc gatgggattt tgcgcgtcag ggcgaaggag ctgcgttcgg
    15421 gcgtagagca ggaggtggaa atgcgctctc agtacggcat ctctgaggag gaaatggccc
    15481 tgatgctgat tgattccatc cagaatgctc agtcggacat ggcggcccgc gccctgctgg
    15541 aggcccgcaa tgaggcccgc agcgtgctgc tggctaccga ccgttttctg attcaaaacg
    15601 ccgaactgtt cagcccggag gaaaaagcgc aaattacagc catcgccggc gaattggacg
    15661 ctgccgtgca gggcgaagat aaagacctca tcaacaaggt gttggaagac ctcaatacct
    15721 actcggcgcc gctggctcac cgggcgttgg aggccaatgt ggcgcaggcc ttgaagggga
    15781 acaaggtgtg aggtgccggc ccgtatgctc gcttgaaagt aagttttcgg gagcttagtg
    15841 aaggggtgac tcagccggta tagccccgta gtcacccctt cactacgcac aagaaggtag
    15901 ttatcgggta cattctttgc tcacacccag cacatacttt tgcaccacgc cgatactgcc
    15961 ggctgtttct gcgccggcaa aaccccgtat catgctgtct ttcaactgag tgcgcgccag
    16021 ttctgtgagc tgccccgata ccggctgata cgaccagtgc catttttcct caaaatatcc
    16081 attggggcgc tccgggcctt tggccgagta tggccgacaa aaaccgtact cgtgggcatg
    16141 ggccgtgagc cactcaaaaa tcttgaggcc ctcgccctga cggaagtacc cgtcggtgag
    16201 ggcgttgatg tcaatatcgg tgccccagtg gtggcgcgag gagccgggca tggaactgta
    16261 ttccagtatt ttgagcgccc gctgcaccgg gtcgggcatg gtttctgcca ggtttttgcc
    16321 gtccaccaag cggctgccgt tccattttgc ctcccagatt tctttttggc gattgaaatt
    16381 gcgtgtagcc gagatgatgc gcagcgttac cccgtcggca gcggcggcgg cgtgcatttt
    16441 tttgaaggct tcgtaggcgt cgcggcgcag gtattggccg gtacggtcgg catattttat
    16501 gtcaatggtg gtgaaatcgg gatgcgaagc gggatcaaat tgtccggtca ggtaggcaat
    16561 ggtgaaggag tcttgccgcg tcgtggtttc ggcttccggc gctgttgttt ccgttagagc
    16621 tgctgtttct gcggcagcgc tgagggccag gtcggtttgg ggcgactgag ggcggcaatt
    16681 gcttagcaac aaagctgtaa ttgccatgaa aatcattgag ttgtgcatag cctgtggttt
    16741 taaacaacaa gggctgcaaa cgacattcgg ctgttggtgc agccggcagc ttgcagcccc
    16801 ggtaaaatgg tatgagattg aaatcagaac tccgagcggc ccggattggc atcgttgaaa
    16861 cgcttggtta cctcgtgcca gttgatgacg ttgaaaaacg cggccacata gtcggggcgg
    16921 cggttttggt agttgaggta gtaggcgtgc tcccacacat cgaggccgag gatgggcgtg
    16981 cctttggcat cgtgaatgtc catcagcggg ttgtcctgat tggcggtgga agtgacgaac
    17041 aggtggtcgt tgccgtccac gcaaagccag gcccagcccg agccgaagcg cgtcatggcg
    17101 gcgttggtaa actgcgtttt gaagttttca aagctgccga aatcgcgttc gatggccttg
    17161 tggatgttca tttgcggagt gggttcgccg ccgccgttgg gcgacatgat ttcccaaaac
    17221 aggttgtggt tgtaaaaacc gcccccgttg ttgcgcactg ccggcgaata cttggaaata
    17281 ttggccagaa tgtcttcaat tttnnnnnnn nnnnnnnnnn nnnnngcgtt gaggttgttg
    17341 gtgtacgctg catggtgttt ggtgtggtgt atctccatgg tgcgggcatc aatatgcggc
    17401 tccagggcgt cgtaagcgta gggtaattcc ggaagttgga atgccatgat cagatgatat
    17461 tttgagtgaa aaaatggtat ttggaaaaat caaaagtaga acgccccgcc ccgtttttag
    17521 ttttcaagag gggcaaggaa aatcaatctc gttttcgcca aagggttggt attttttgca
    17581 tggcttagcg aaggggtgac tcgcag
//
LOCUS       DAORLF010000038        17325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1763_length_17325_cov_2.036943, whole genome shotgun sequence.
ACCESSION   DAORLF010000038 DAORLF010000000
VERSION     DAORLF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_17325_cov_2.036943"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>382)
                     /locus_tag="PK198_05425"
     CDS             complement(19..>382)
                     /locus_tag="PK198_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF38202.1"
                     /translation="PDNGTTNNGLDAASTTDLGVFEAINNPAFLGAFKVPSLKNIELS
                     APYMHDGRFNTLEEVIEHYNSGVQNHPNLSNALKNGAGQPIRLNLTAQQKTDLVNFLK
                     TLTDQQLTNDVKFSNPFK"
     assembly_gap    383..392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<393..>731)
                     /locus_tag="PK198_05430"
     CDS             complement(<393..>731)
                     /locus_tag="PK198_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF38203.1"
                     /translation="VLMPFQDPVEMGMTLDEVVRAVQAQSFYPELFKDAFGTPDITEQ
                     RISRALAQFVRSIVSVESKYDTGRQGVSAPTNNFANFSASENNGKRLFFLPVSAGGGG
                     CVGCHSTEAF"
     assembly_gap    732..777
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<778..1198)
                     /locus_tag="PK198_05435"
     CDS             complement(<778..1198)
                     /locus_tag="PK198_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF38204.1"
                     /translation="MKTSSMMVILTAALALFVACDKETDDGNPISDILNLPATPYNYT
                     AINLPAHLTTNALLGPGQNAAVNNDNTPASNPTTNEGATLGRVLFYDKKLSANGTVSC
                     ASCHKQEQAFSDNARLSLGFSGGTTRRHSMSLVNARWY"
     gene            complement(1462..2040)
                     /locus_tag="PK198_05440"
     CDS             complement(1462..2040)
                     /locus_tag="PK198_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF38205.1"
                     /translation="MHKIILLLLAFISIGMVQAQTALIKDPDGYCNVRDSASVRSKVL
                     DTLHEGRIIFVYPEEAKGAWLLVDYFKGSKMLTGYIHTSRVVLLNNLTAFKPVAANDT
                     LLQFQLDSFQIKIIQGKFVRQGRQLKYATPQGQQTFIKSIDGKFPWGTDGNLPRFEYK
                     HIEMKWGLETLIFPPESFRDLFEPNFHLTSAP"
     assembly_gap    2072..2174
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2321..>3085)
                     /locus_tag="PK198_05445"
     CDS             complement(2321..>3085)
                     /locus_tag="PK198_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRF38206.1"
                     /translation="QNIPIRTPEGAEVRKAFIPRDENHILLSADYSQIELRIIAEISG
                     DQAMLDAFTAGQDIHRATAARVFGVSFDEVSTEQRYRAKTVNFSIIYGAGATNLSRQL
                     NIKRAEAGELISQYFNQYPSLKAYMENTVQHARDHGYVTTIMGRRRYLRDIHSKSSLE
                     RSNAERIAVNTPIQGAAADMIKIAMVNIHNDLRSQGLHTKMILQVHDELVFDVPKSEL
                     DIVRPIIEERMRTAIPHLKVPIEVGMGTGNTWLEAH"
     assembly_gap    3086..3173
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3174..>3706)
                     /locus_tag="PK198_05450"
     CDS             complement(<3174..>3706)
                     /locus_tag="PK198_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRF38207.1"
                     /translation="PVSIETLIGKKGKQQITMRDAPLEEVKEYAAEDADITLQLRRHF
                     EPMLEKENVGELFHNIEAPLVKVLADMEFEGVRINPAFLENYSGQLAAQIVALEDSIY
                     QQVGAAFNIGSPKQVGEILFDRLRIPYPGKKTKSGQYSTDEEILTDLSKNHPVAADIL
                     KHRGLNKLKSTYVDALP"
     assembly_gap    3707..3829
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3830..>4120)
                     /locus_tag="PK198_05455"
     CDS             complement(<3830..>4120)
                     /locus_tag="PK198_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRF38208.1"
                     /translation="HTYHLADTPELRAQLIGLLAAETQFCFDTETTHIDANQAEIVGM
                     SFSVQPAEAWYVPVPADQTEARALLEEFRSVLENESIEKTGQNLKYDAIILK"
     assembly_gap    4121..4175
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4176..5146)
                     /locus_tag="PK198_05460"
     CDS             complement(<4176..5146)
                     /locus_tag="PK198_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRF38209.1"
                     /translation="MSAKRLFLLDGHALVYRAHFAFITRPLINSKGINTSAVTGFTRQ
                     LWDLIRTEKPTHLAVVFDPPTPTFRHEMYEPYKANREETPDDIRTAFPYIRRIIQGFN
                     IPVLSLDGYESDDVIGTVARQAEAQGYTVFMVTPDKDMGQLVSPHVFMYKPSRQGNGI
                     EIWGEKEVTENWDIARVEQVIDILGLQGDAVDNIPGIPGVGPKTAAAMLKSFGSVENI
                     IANADQLKGKQKELVQTYAEQALLSKKLATIEVNAPITFDPTAFQMDPINREALAEVF
                     KELEFRTLAVQILGEGDSPSAPASKPASGGGVQGSLFDDSAMEAAPGP"
     assembly_gap    5187..5318
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5464..>5749)
                     /locus_tag="PK198_05465"
     CDS             complement(5464..>5749)
                     /locus_tag="PK198_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38210.1"
                     /translation="VKRLHGQPVLIEGFYIPFEAEESGETFYILSQYPNSQCFFCGAA
                     GPDTVMDLMMKSPPGHLKMDDRVAFKGRLRVNDSDVMFLNYILDDAEWVK"
     assembly_gap    5750..5766
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5767..6003)
                     /locus_tag="PK198_05470"
     CDS             complement(<5767..6003)
                     /locus_tag="PK198_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38211.1"
                     /translation="MKTAMLLSIFLGLTITACAPAAPDAQNETPPAPEQEADTPPPDW
                     PMKDGRYVLNWPDLAIPDYRTEFNEEIGQEVSIPL"
     assembly_gap    6321..6332
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6333..>6664
                     /locus_tag="PK198_05475"
     CDS             <6333..>6664
                     /locus_tag="PK198_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRF38212.1"
                     /translation="SAKLVSENVVEIKVNALHNKKASSYVAVFNIVQLGRTTEETNTL
                     LNNRLNAFVNGVKALGVPASDVYVDMVNFLPKYEIDATKKLFSKKTYTEVPKGFELQK
                     NVHIRYTD"
     assembly_gap    6665..6674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6675..7159
                     /locus_tag="PK198_05480"
     CDS             <6675..7159
                     /locus_tag="PK198_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRF38213.1"
                     /translation="VVTAAAAQEIYDLVKVDYFDDNPTAAYEELRQKSFEYLTKVRQD
                     FLKAGIELDSAYAIAAENTWVAFPGNRYESYQAYSTQSFEPIDKNAAVSTVDKPVSRF
                     YNAVPANDYDIILQPEILVIQYSYNLVVRFTLPAKTKAQPKSYFIITPSGQLQPVKLD
                     "
     gene            complement(7406..>8121)
                     /locus_tag="PK198_05485"
     CDS             complement(7406..>8121)
                     /locus_tag="PK198_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRF38214.1"
                     /translation="REFTTHTGRIGGKRISVVSTGIGPDNIDIVVNELDALANIDLKT
                     RTLKPEHTTLDIIRVGTSGGIQPDLEVGDFVVSTYAIGLDNLMYFYDWQHHLEEAETQ
                     DAFYDFLRETGPLPVRPYLFGGSPALIQEVGSRMHRGITLTSPGFYGAQGRQLRAPGR
                     LSGAAIERLSYFQFGGHRITNFEMETAAIYGLCRVLGHRAASCSVLLANRTRNTFSDN
                     PAAIVDRLIEEVLGAIGGR"
     assembly_gap    8122..8148
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8351..8701
                     /locus_tag="PK198_05490"
     CDS             8351..8701
                     /locus_tag="PK198_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRF38215.1"
                     /translation="MKKIYHLGNCGTCQKILAELDGGRGCELQEIKTQKITAEQLDAM
                     KELAGSYEALFSRKAMKFRSMGLAEKQLSENDYRQLILDEYTFLKRPVIVVGDKIFIG
                     NAAKSVQAAKSALG"
     gene            8783..9022
                     /locus_tag="PK198_05495"
     CDS             8783..9022
                     /locus_tag="PK198_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38216.1"
                     /translation="MNLFSNWHFMRIVRALIAIWAVFEFVNTKEWTVLFIGGFFAVQA
                     IFDVGCCGASGCATAPQPNKQQELSIQEVEYEEVK"
     assembly_gap    9216..9230
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9231..9744)
                     /locus_tag="PK198_05500"
     CDS             complement(<9231..9744)
                     /locus_tag="PK198_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRF38217.1"
                     /translation="MILTNRLFTRREPVKEAKSVYIFCEGKKREHQYFRYFQGIDSRI
                     NIVIHELDSHENNSPTGLYQLAVRHLIKSDDNPNPPFELWDNDEVWFVIDTDAWALKT
                     TELRSFCQTRPNWNIAQSNPCFEVWLYFHFFDSRATFQWLEICDAWKQYLSQTIPGGF
                     NSNKHPIFIGA"
     gene            complement(9731..11089)
                     /locus_tag="PK198_05505"
     CDS             complement(9731..11089)
                     /locus_tag="PK198_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF38218.1"
                     /translation="MLIRLVIQNVYSFFESKEFNMLPRPKIRTPEGHKYTLNGLELLK
                     LSAVYGANAAGKSNLVKALGLLQYLATGAATSSKVHEAHFKFDDATPCKQVLAVEFVQ
                     EGKPYYYAVEITDGAIATEELYRSGLGKAADVLIFERKKMGETVHLTFSDAFEKDAES
                     RMLKSILIHEFLEPQETILPWLANRQNPYLNEAKAAFQWFSQTLQIINPETKVNALSL
                     RLDTDQPFKSFANDLLCAYGLGISKIDVEEMPLKEIFGENNEAAISAVRKKMGETKNG
                     FLTIPSRTGDELIIARKNDDFVAKHLTVTQSNKGGNEAAFNIDELSDGTIRLLDFLPL
                     FQGVADRPKVYVVDELERSIHPSLAKELVTKFSHDKDTKGQLIFTTHESNLLDQSIFR
                     QDEVWFAEKSPDGSTDLYSLSDFKEHHTIDIRKGYLNGRYGAVPFLANLHELNWHAYD
                     TH"
     gene            11482..12267
                     /locus_tag="PK198_05510"
     CDS             11482..12267
                     /locus_tag="PK198_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38219.1"
                     /translation="MKASITAFVFFIVVIGPISAQDSTARNSSWSGLLTLATKNVWRG
                     IDFGNGTPSAQGLVTFKPSDRWDFNVLGITSLTGDNRGYSTTLNFFVNYTLGDLTLTL
                     DNYYFEGDETNIPTSFWDYRNTHFLEGRVGCALGKFSLTAAYTLYGGGVYSNPVIDSV
                     GTVLQNTRGLYLEAKYQPSEEITLSIGGLTGASALNFNDKAGLTNIGFKYSKSLKITD
                     HFSIPFDFQLVVNPSWSNIAPAGLPRVGYGNQRVNFSVAMTLE"
     gene            12693..>13167
                     /locus_tag="PK198_05515"
     CDS             12693..>13167
                     /locus_tag="PK198_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38220.1"
                     /translation="MRNVIAAFIIMIVAVMTAHTQSALRPRQLVEQAHLEGKSFTPIS
                     LFERAINFRSEVEITPIEDYTLLNLDSEGLRQLAAAPPAALLLELPFESGRLQLELVR
                     TDADPIPVRESQPTDFGRNSFTGLHYRGVVRGQEGSVAAVSFVAGEVMGLVSSRQE"
     assembly_gap    13168..13230
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13231..>14365
                     /locus_tag="PK198_05520"
     CDS             <13231..>14365
                     /locus_tag="PK198_05520"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRF38221.1"
                     /translation="APVLEAMEMDCHTADDGPDYLPHQLQEVLEFRNEAKCTRIYFEV
                     DYDIFTDKGGTAGAISYVTALFNQVAALYANENIGIAISEIFVWTTNSPYMGSNPGTL
                     LNQIQTLRTQFNGHLAHLLSYKPSGGIAVLSGLCHPLTIAKFGVSGIRSTFSNVPTYS
                     WSVMVIAHELGHQFGSKHTHACAWNGNNTAIDGCAGFVEGSCALPPMPAVGTGTIMSY
                     CHVTSVGINLSLGFGAQPGNVMRNAVAAAPCLPVCTTSGGGGGGGNPTPTCANNTVIL
                     RLVLDNYGPETTWRLTNSTGQVVKSGGPYPKGMAGTAILDTMCLPNGCYTFRILDTYG
                     DGICCNYGNGSYTLRNAAGQVLITGGQFGFEESKSICVPNTPPA"
     assembly_gap    14366..14383
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14384..>14702
                     /locus_tag="PK198_05525"
     CDS             <14384..>14702
                     /locus_tag="PK198_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38222.1"
                     /translation="TGCVQVNFAQTPVVSYGGPEDLGSFTLLSSNTVLRIQNNAWKAV
                     MLNYNVTPNTKLQFDFASTAPGEIHGIGFDNDSIIGPERTFKLYGSQNWGITNFNNYP
                     GYNS"
     assembly_gap    14703..14755
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14756..15163
                     /locus_tag="PK198_05530"
     CDS             <14756..15163
                     /locus_tag="PK198_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38223.1"
                     /translation="FNRLFFAADHDASPANGNSFFRNIRIYEGTACSNLIPDNEMELA
                     ALLEEEPGGQRVQLFPNPVSDELQLRFTAPLSADEPVILRIFSAAGQLVQQETLSSAQ
                     AKQPVQVAQLRSGAYLLHIQSGAYSENLKFIKH"
     gene            15469..16332
                     /locus_tag="PK198_05535"
     CDS             15469..16332
                     /locus_tag="PK198_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38224.1"
                     /translation="MKKQLWILLCLLPLALSAQKNIFQWQPARYDATETLAAELEHWE
                     IFQINPLEMSRLARQYPQGADMSWQWPTGEVTFQLFADDIRASGYRSAELSAGGVREL
                     PAGPNITYGGQVAGGGNMRLTLHDDFIFGYWEKDGQIWFVQPLRHFVPSAPEHHFVIY
                     KTSDVKPVPNAHCGWTHAEQHRKQHAEDVQHAKMAGECYQVEIAIASDFLMFQFFGTV
                     GAVQDFTIGVLNTVQTNYDNDFADEIRFLIVANMVSSCATCDLDCLHRVQRAVGVVPR
                     LGQRRRIWCKL"
     gene            16265..>16628
                     /locus_tag="PK198_05540"
     CDS             16265..>16628
                     /locus_tag="PK198_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRF38225.1"
                     /translation="MSNELLVSFRAWGNGGGFGVNYDVASLWSDRDFNGNTIGLAWVG
                     ALCSGSRYNILERFTSNSAFLRNLQAHELGHNFDADHDATGSPTIMAPSVNGSNNWSA
                     LSVSVINNYITFIAGQGDC"
     assembly_gap    16629..16654
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16655..>17325
                     /locus_tag="PK198_05545"
     CDS             <16655..>17325
                     /locus_tag="PK198_05545"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF38226.1"
                     /translation="QPLAPTAQIQLPVTHVCPGSVVPLIDNSAGTPTSWNWGLSGATP
                     PSSFLQSPTVTYDNPGTYPVVLTVSNAIGSDTQFSNTDIVVDENGQKFLLYETFENPI
                     LYWDIVNPDNGITWDVREVGGSPYGNKAAYINSAGYSGSGQVDGMISPSFSLSGVSNP
                     VLKIDYAYRRRTSSGSEQLRVKVSTNGGATFPNTIFTGQENGSGNFATGGVLGSAFTP
                     ASASD"
BASE COUNT         3740 a         4250 c         4466 g         4071 t
ORIGIN      
        1 atttggaaaa atgtccaatc atttgaatgg attactgaac ttaacgtcat tcgttaactg
       61 ctggtcggtc agtgttttta gaaaattgac caaatctgtt ttttgctgag ccgtcaggtt
      121 taaccgaatc ggttgtccgg ctccattttt cagtgcattg ctcaagttgg gatgattttg
      181 aacgccgctg ttgtagtgct cgatcacctc ttccagtgta ttgaatctgc catcgtgcat
      241 gtagggcgca ctcagctcga tgtttttgag cgagggcact ttaaaagctc ccaaaaacgc
      301 cggattgttg atggcttcaa aaacgcccaa atccgtagtg gaagccgcat ccaaaccatt
      361 gttggtggtg ccgttgtcgg ggnnnnnnnn nntaaacgcc tcggtcgaat ggcagcctac
      421 acagccgccg ccgcctgccg atacgggcaa aaagaaaagg cgcttaccgt tgttttcgct
      481 ggcgctgaag ttcgcaaaat tgttggtggg agcactgacg ccctgcctgc cggtatcgta
      541 tttgctctcg acactcacga tgcttctcac aaactgagcc aatgccctcg atatgcgctg
      601 ctcggtgata tcgggcgttc caaaagcatc tttaaatagt tcaggataaa aggattgcgc
      661 ttggacagcg cgtaccactt catctaaggt catgcccatt tctacggggt cttgaaacgg
      721 catgagcacc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat
      781 accagcgggc attgaccaat gacatggagt gccttctcgt cgttcctccg ctaaatccta
      841 agctcagcct ggcattgtct gaaaatgcct gttcctgctt gtggcaagac gcacaagaaa
      901 cggttccatt ggcgcttagt tttttatcat aaaaaaggac ccttcccagc gtggcgcctt
      961 cgttggtagt aggattggag gccggcgtgt tgtcgttgtt cactgccgca ttttgccccg
     1021 gtcccaagag tgcattggtt gtcaggtggg cgggcaggtt gatggccgtg tagttgtaag
     1081 gagtggccgg gaggttgagg atgtcggaaa tgggattgcc atcatctgtt tctttgtcgc
     1141 aggcaacgaa caaagccagg gctgctgtca gtattaccat catactactt gtcttcatgt
     1201 gcggcatttt tttgagtaat caatagggct tagatgagaa aagcggcggg aggtttaacg
     1261 ggggtggagg caagggggga attatttccg acttggtgaa ggtaaatgcg acgcattcct
     1321 ttgcagggac taagcgggct tcaaaaaggg atgaaggttt gtcggcctgc tatctgcccg
     1381 ttttggatga tccagaccac agcataggcg ccggcggcat cgctattcat tgcttctaca
     1441 tagactttgc ctgttgactt gtcacggtgc ggacgttaga tggaaattgg gttcaaacaa
     1501 gtctctgaaa ctttccgggg ggaaaatcag tgtttcgagt ccccatttca tctcaatatg
     1561 tttgtactca aagcgtggca agttgccgtc cgttccccag ggaaattttc cgtctatgga
     1621 ttttataaaa gtctgttggc cttgaggcgt agcgtatttg agttgtcgcc cctgtcggac
     1681 aaattttccc tgtataattt ttatttgaaa actgtccaac tggaattgca gcagcgtatc
     1741 attggctgct accggtttaa aggcagtcaa gttattcagc aatacaaccc ttgatgtatg
     1801 tatgtaccca gtaagcatct tgcttccttt gaagtaatca accagcagcc atgcaccttt
     1861 agcctcttcg gggtaaacga aaatgattct gccctcatga agcgtgtcca gcaccttcga
     1921 acggacgctt gcactatctc gtacattgca gtagccgtca gggtctttta tcagagctgt
     1981 ttgcgcctgc accatcccga tgctgatgaa ggcgaggagt aaaaggatga ttttgtgcat
     2041 gtggtgagcg cttgcagggt gaatgttgta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnataggc gaccttagga gcctattgta gccgatgacc cggcgcccgc
     2221 agcgggtaca gagctgttag ctttagcggg ttattgcggt gataaagcta acagctctgt
     2281 tggtggagcg ggcggccgcc caaagaaaaa aacttatgcc tcaatgcgcc tccaaccatg
     2341 tatttcccgt gcccatgccc acctcgatgg gcactttgag gtgggggatg gcggtgcgca
     2401 tgcgctcttc gatgatgggc cgtacgatgt cgagttccga tttgggcaca tcgaagacga
     2461 gttcgtcgtg tacctgaagg atcattttgg tgtggaggcc ctggctgcgc aagtcgttgt
     2521 gaatgttgac catagcgatt ttgatcatgt cggcggcggc gccttgaatg ggcgtgttga
     2581 cggcgatgcg ttcggcgttg gagcgttcga gggagctttt ggagtggatg tcgcggaggt
     2641 agcggcggcg gcccatgatg gtggtgacgt agccgtggtc gcgggcgtgc tgcacggtgt
     2701 tttccatgta ggctttgagg gaggggtact ggttgaagta ctgcgagatg agttcgccgg
     2761 cttcggcgcg tttgatgttg agctggcggg agaggttggt ggcgccggcg ccgtagatga
     2821 tgctgaaatt gacggttttg gcgcggtagc gctgctcggt gctcacctcg tcgaaggaca
     2881 cgccgaagac gcgggctgcc gtggcgcggt ggatgtcctg gccggcggtg aaggcgtcga
     2941 gcatggcctg gtcgccgctg atttcggcga tgatgcgcag ttctatttgc gagtagtcgg
     3001 cggagaggag gatgtggttt tcatcgcggg ggatgaaggc tttgcgcacc tcggcgcctt
     3061 cgggcgtgcg gatggggatg ttctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgggca
     3181 gggcgtccac ataggtggat ttgagcttgt tgaggccgcg atgtttgagg atgtcggcgg
     3241 cgacgggatg atttttggag agatcggtga ggatttcttc gtcggtagag tactgccccg
     3301 atttggtttt tttgcccgga taggggatgc ggaggcggtc gaagaggatt tcgcctacct
     3361 gcttgggcga accgatattg aaggcggcgc ctacctgctg gtaaatggaa tcttcgaggg
     3421 cgacgatctg ggcggcgagt tggccggagt agttttcgag gaaggccggg ttgatgcgca
     3481 cgccttcgaa ctccatgtcg gccaacactt taactaaggg cgcttcgatg ttgtggaaca
     3541 gttcgccgac gttttctttt tcgagcatcg gctcaaaatg gcggcgcagt tgcagggtga
     3601 tgtcggcgtc ttcggcggcg tattctttca cctcttcgag gggggcatcg cgcatggtga
     3661 tctgctgttt gccttttttg ccgatgagcg tttcgatgga taccggnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tttgaggatg
     3841 atggcgtcgt atttgaggtt ttggccggtt ttttcgatgc tttcgttttc cagtacgctg
     3901 cgaaattcct ccagcagggc gcgggcctcg gtttgatcgg cgggtacggg tacataccat
     3961 gcttcggcgg gctgaacgga gaaggacatg cccacgattt cggcctgatt ggcatcaatg
     4021 tgggtggttt cggtgtcgaa acaaaactgc gtttctgcgg cgagcaggcc gatgagttgg
     4081 gcgcgcagtt cgggtgtatc ggccaggtgg taagtgtgcg nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggcc gggcgcggcc tccatggcgg
     4201 agtcgtcgaa gagggagcct tgtacgccgc cgccggaagc gggtttggat gccggtgctg
     4261 agggggagtc gccttcgccg agaatctgca cggccaaagt tctgaattcc aattctttaa
     4321 atacctcggc cagcgcttcc cggttgatgg ggtccatctg gaaggcggta ggatcgaaag
     4381 tgatgggcgc gttcacctca atggtggcga gctttttgga aagcagggcc tgttcggcgt
     4441 aggtttgtac caattccttt tgtttgcctt tgagttggtc ggcattggcg atgatgtttt
     4501 ccaccgagcc aaaggatttg agcatggcgg cagcggtttt cggccccacg ccgggaatgc
     4561 cgggaatgtt gtccacggcg tcgccttgca ggccgaggat gtcaatgacc tgctccacgc
     4621 gcgcgatgtc ccagttttcg gtcacttctt tttcgcccca gatttcgatg ccgttgccct
     4681 gccgggaagg tttgtacatg aagacgtgcg gcgacacgag ctggcccatg tctttgtcgg
     4741 gcgtgaccat aaaaacggtg tagccctggg cttcggcctg gcgcgctacg gtgccgatga
     4801 cgtcgtcgga ttcgtagccg tccaacgaca aaacagggat gttgaagccc tgaatgatgc
     4861 gccggatgta agggaaagct gtgcggatat cgtcgggggt ttcttcgcgg ttggccttgt
     4921 acggctcgta catttcgtgg cggaaggtag gcgtgggcgg atcgaatacc actgccagat
     4981 gggtgggttt ttcggtgcgg atgaggtccc acaactgtcg ggtgaaacca gtgacggcgg
     5041 aggtgttgat gcctttggag ttgatgagcg gccgggtgat gaaggcgaag tgcgcccgat
     5101 atacgagggc gtggccgtct aagaggaaca atcgttttgc agacatggcg agggtgattt
     5161 tgagggggca agatagggga tttttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ccgcgccacg gtgcggctac
     5341 cggttccggg ttcaaggtgt cgggttggaa caggtggctg ctggcttcaa gctgctggct
     5401 tcaagccgcg cttaccgcgc cacggcgcgg ctaccgaata ccgcctaccg attttccgct
     5461 cattcacttt acccactccg catcgtcgag gatgtaattg aggaacatca catcagagtc
     5521 gttgacgcgc aggcggccct tgaaggccac gcggtcgtcc attttcagat gaccgggagg
     5581 cgatttcatc atcaggtcca tgaccgtatc ggggccggcg gcgccgcaga aaaagcactg
     5641 cgaattggga tattgcgaaa gaatgtaaaa ggtttcgccg gactcctcgg cttcaaaagg
     5701 aatataaaaa ccctcgatga gcacaggctg cccgtgcagg cgcttcaccn nnnnnnnnnn
     5761 nnnnnncagc ggaatgctca cctcttgccc gatttcttcg ttgaattcgg tgcgataatc
     5821 gggtatggcc agatcgggcc agttcagcac atacctgccg tctttcatgg gccagtcggg
     5881 cggcggcgtg tcggcttcct gttccggcgc gggcggtgtt tcattttggg cgtcgggcgc
     5941 agcaggcgcg caggccgtga tggtcaggcc caggaagatg gaaagtagca tggcggtttt
     6001 catggcgcaa ttttcaggat tttattttaa aatcgcacag tatagcagtc ttcagaatga
     6061 cacaaacacc gaaaaacgca ttttccgcca gggcgagcat ttagacgccc cgcaattcgg
     6121 ccttcgcatc gccctcagcg aattgctgcc gccacaataa acacagcggc ccgcttcgtt
     6181 acagctctaa aaaacatcac catgcgctca tttttccttt tcagtatgct gctgtgcgtt
     6241 caattgcttt gggggcaggc agccggcaat tacatgcaat acaacaattt tgatccaagc
     6301 caaaatgctc gttatcagtc nnnnnnnnnn nnaatcggcc aaattggttt cggagaatgt
     6361 cgtggagatc aaagtcaacg cactgcacaa caaaaaagcc tcctcctatg tggcggtgtt
     6421 caatattgtg caattgggcc gcaccaccga agagaccaac accctgctca acaaccgcct
     6481 caacgctttc gtcaacggcg tgaaagcatt gggcgtaccc gcttccgacg tgtatgtgga
     6541 catggtaaat tttctgccca aatacgaaat tgacgccacc aaaaaactct tcagcaaaaa
     6601 gacctacacc gaggtgccca agggctttga actccagaaa aacgtacaca tccgctacac
     6661 cgatnnnnnn nnnnaggtgg tgaccgccgc cgccgcacag gaaatttacg acctcgtgaa
     6721 agtggattat ttcgacgaca accccactgc ggcttatgag gaactgcgcc aaaaaagctt
     6781 tgagtatctg accaaagtgc gccaggattt cctcaaggcc ggcattgaat tggacagcgc
     6841 ctacgccatc gccgccgaaa atacctgggt ggcttttccc ggcaatcgct acgagtctta
     6901 tcaggcgtac agcacgcagt cctttgaacc cattgataaa aatgccgccg tgagcaccgt
     6961 ggacaagccc gtgtcgaggt tctacaacgc cgtgcctgcc aatgattacg acatcatcct
     7021 gcagcccgaa attctggtca tccagtattc ttacaacttg gtggttcggt tcacgcttcc
     7081 tgccaaaacc aaagcgcagc cgaagtctta cttcatcatc acgccttccg gccaattgca
     7141 gccggtgaag ttggactgat tttggattgc agtaaaatga ggtgactatt cagtagcttt
     7201 tggagacttg ctgaatagtt acaaaatgaa tttatcagtc agctacgagc catgagctgc
     7261 gaactgagat gttcgaagag gataagcact ttcctttcgg ggaaattatg cctttgtggt
     7321 attgaaaatg aatctttttc aacgtgggga cacaaactcg aagctcaaag ctcgcggctc
     7381 gaagctatac aagtagtacg gcggcctacc ggccaccaat agctcccaac acctcctcaa
     7441 tcaaccgatc cacgatggcc gcagggttgt cggaaaaagt attgcgggtg cgattggcca
     7501 acaatacact gcacgatgcc gcccggtgcc ccaacacccg gcacagacca taaatagccg
     7561 ccgtttccat ctcgaaattg gtgatgcgat gccctccgaa ttggaagtag ctcagccgct
     7621 cgatggccgc accgctcagc ctgcccggag cgcgcaattg ccgtccctgt gcgccgtaaa
     7681 accccggact ggtgagcgtt ataccccggt gcatacggct gccgacctcc tgaatgagag
     7741 ccgggcttcc gccaaacagg tagggcctga cgggcagcgg gccggtttcg cgcaggaaat
     7801 cgtagaaggc atcctgcgtt tctgcttctt ccagatggtg ttgccaatcg taaaaataca
     7861 tgaggttgtc taagccgatg gcgtaggtgg acaccacaaa atcgcctact tccaaatcgg
     7921 gctggatgcc gccggaggtg cccacccgga tgatgtcgag ggtggtgtgt tctggtttta
     7981 aagttctggt tttcaggtca atgttggcca gagcatccaa ttcattcacc acaatgtcta
     8041 tgttgtcggg gccgataccg gtagaaacga cgctgatgcg cttacccccg atgcgcccgg
     8101 tatgcgtggt aaattcccgg tnnnnnnnnn nnnnnnnnnn nnnnnnnncg aagtgctgag
     8161 acacccgctc cacccggccc ggatcgccta cggtgatgat ggtgtcggct atttgttcgg
     8221 ggtgcaggtg caggtgataa atgctgccat cgggatgcag gacgagttcg gagggcggaa
     8281 attgagtcat attgcgttga acctttttgt agtgcaatga ttttagtgag gacaatcaaa
     8341 cacaagtgaa atgaaaaaga tataccacct cggaaactgc ggaacctgcc agaaaatctt
     8401 agcggaattg gacggcgggc gcggctgcga attgcaggaa atcaaaacgc aaaaaatcac
     8461 cgccgaacaa ttggacgcta tgaaggaact ggccggctcg tacgaggcac tgttcagccg
     8521 caaagctatg aagtttcgtt cgatgggctt agccgaaaaa caactcagcg aaaacgacta
     8581 ccgccaactc atccttgatg aatacacctt cctgaagcgg cccgtcatcg tggtgggcga
     8641 caaaatcttc atcggcaatg cggccaaaag cgttcaggcg gcgaagtcgg cgctgggctg
     8701 agtaaaactt acgctctgtg tgatttgggt tacgttgcag gccgctaagc aagcataagt
     8761 ttgcccgaaa tttcacaaca atatgaacct gttttccaac tggcatttca tgcgcatcgt
     8821 tcgggcgctt atcgcaatat gggctgtttt tgaattcgtc aataccaaag agtggacggt
     8881 gctgttcatt ggaggatttt ttgccgtgca ggccattttc gacgtcggct gctgcggcgc
     8941 atcgggctgc gctaccgctc ctcaacccaa taaacagcag gagctttcga tacaagaggt
     9001 tgaatatgag gaggtgaagt agtgataagg gggctgcgct cccaagtgta taaatgtcgg
     9061 aaaagtatga agtgtcggca tcaaatacga ttccgaatgc tctctatttt ttttccgcaa
     9121 aactcaaaaa tcatcttagc caaaaggaaa acctctgagc acccgacggg cggcccgcca
     9181 ttttcggata tgaattgccg cccggcattt tcaatnnnnn nnnnnnnnnn cagcgccgat
     9241 gaaaatcgga tgcttgttgg agttaaagcc gcctggaatt gtttgagata aatattgctt
     9301 ccaagcatcg caaatttcga gccattgaaa tgtggcacgg ctatcaaaaa aatgaaaata
     9361 tagccaaacc tcgaagcatg gattgctctg agcaatgttc caattagggc gagtttgaca
     9421 aaacgacctt agttcggtgg tttttaaagc ccaggcatcg gtatcaatta caaaccagac
     9481 ctcgtcgttg tcccataatt caaatggagg attgggatta tcgtcacttt tgatcaaatg
     9541 tcgcactgct aattggtaaa gtcctgtagg cgagttgttt tcgtgtgagt ccaattcgtg
     9601 aattacaatg ttaatacggc tgtcaatacc ctgaaagtat cggaaatatt ggtgttcacg
     9661 ttttttgcct tcacaaaaaa tataaacgct tttggcctcc tttacgggtt ctcggcgtgt
     9721 gaataagcga ttagtgagta tcataggcgt gccagttcaa ttcgtggagg ttggctaaaa
     9781 agggcacggc gccgtatcgc ccgttgagat agccctttcg gatgtcaatg gtatgatgct
     9841 ccttaaaatc gctcaatgaa tataagtcgg tgctgccatc gggacttttt tcggcaaacc
     9901 aaacctcatc ctgccggaat atggattggt cgagaaggtt ggattcgtgt gtggtgaaga
     9961 ttaattgccc ctttgtgtct ttatcgtggg aaaatttggt taccaattct tttgccagcg
    10021 acgggtgtat actacgttct aattcatcta ccacatacac tttgggcctg tctgccacgc
    10081 cttgaaacaa gggaaggaaa tctaacaacc ggatggtgcc gtcagagagc tcgtcaatat
    10141 tgaatgcggc ctcatttcct cccttattgg attgagttac agtcaaatgt tttgcaacaa
    10201 aatcatcgtt ttttcgggca atgattaact cgtcgcccgt tctgctgggt atcgttagaa
    10261 atccattttt ggtttctccc atttttttac gcacagcgct tattgccgct tcgttgtttt
    10321 cgccaaatat ttctttaaga ggcatttctt caacgtcaat tttggaaatg cccagaccat
    10381 aggcacaaag caaatcattt gcaaatgatt tgaatggttg atccgtgtcc agtcgaagcg
    10441 acaaagcatt aactttagtc tccggattga tgatttgaag tgtctggctg aaccattgga
    10501 aggcggcctt tgcctcattc aaataggggt tttgccgatt agcgagccaa ggcaatatgg
    10561 tttcttgggg ttctaaaaat tcgtgtatga gtatagattt caacatcctg ctctcagcgt
    10621 ctttctcgaa ggcgtctgaa aatgtcagat ggacagtttc tcccattttt ttgcgctcga
    10681 aaatcagcac gtcggcagcc ttccccagcc cggaccggta aagttcttcg gtagctattg
    10741 ctccatctgt aatttctaca gcataataat aaggcttccc ttcttgaaca aactcaacag
    10801 caagcacctg tttgcaggga gtagcatcgt caaacttaaa atgtgcttca tgaaccttac
    10861 tgctggttgc agccccagtt gccaaatatt gcagtagccc aagtgccttg actaaattgg
    10921 attttccggc ggcatttgct ccgtaaactg ccgagagttt aagcagttcc agaccattca
    10981 aggtatattt atgtccttcc ggcgtcctga ttttaggtcg gggcagcata ttgaactctt
    11041 tgctttcaaa aaaggaatat acattttgaa taaccagtcg aatgagcata aagtgaaaat
    11101 tttacacaaa gttaaattgt tttgcttttt ttgtcaagtt tttattttgt ctatttgata
    11161 gtttggtgaa aatttcacca aatttggttc gtttaactat ggtatatacc aagtatgata
    11221 cttggcgctg ccaatcacct cgccatcgaa ggctatgcac aaaaccgggc ggcgcaaggt
    11281 gtaaagtttt catgctgact gcgcagggct agcgacccaa cgcgcatagc atcggcgcgg
    11341 ttcatgcggc gttttcgctt gatgttttgt ttgaaaaggt ttgaatggcg tattgccccc
    11401 ccaaaaataa cccctattca cttagccctt tccatcttgt tgataatcat tacttttgca
    11461 cccaacctaa aatcatttgc catgaaagcg tctataactg cctttgtttt ttttatcgtc
    11521 gttatcggcc caatcagcgc gcaagacagt accgcccgca acagctcctg gagcggactg
    11581 ctcactttag ccaccaaaaa tgtatggcgc ggcattgatt tcggcaacgg cacccccagc
    11641 gctcagggct tggtcacttt caaaccttcc gatcgctggg atttcaatgt gctgggcatc
    11701 acatcgctca ccggcgacaa cagggggtat tctaccacgc tcaatttttt tgtcaattac
    11761 acgctgggcg acctcacgct caccttagac aattactact tcgagggcga tgagaccaac
    11821 attcccacga gtttctggga ctaccgcaat acgcatttcc tggagggccg ggtgggctgc
    11881 gcgctcggta aattcagtct caccgcagct tatacgcttt atggcggcgg cgtttacagc
    11941 aatcccgtca ttgactccgt tggtacggtt ttgcaaaaca cccgaggctt atacctcgag
    12001 gccaaatacc agcccagtga ggaaatcacc ctcagcatcg gcggcctcac aggagccagc
    12061 gctttgaatt tcaacgacaa agccggcctg accaacatcg ggttcaaata ttccaagtct
    12121 ttgaaaatca cagatcattt ttccattccg tttgatttcc agttggtcgt caacccgtcc
    12181 tggagcaaca tcgctccagc cggactgccc agggtgggat atggaaacca gcgcgtgaat
    12241 ttttctgttg cgatgacatt agagtaggat tccctacgcc ctcccccata atttgcaacc
    12301 gctttctaaa ccaatcgcca cataaaccgc cgtattgtgc ccgcaaccaa aaccggcccc
    12361 gaaaccgcgt cataacccga aaatataatg tggagaccct cgttttttat ctcgttatct
    12421 gtggccacac caccctgaat aactgagaga aaacaaccca tctatccatt gagagaaaac
    12481 tatcaagcag caacccgctg tgcgaaggca agacatgtat tgcccgatgc gcggcaggta
    12541 gcacccgcaa cggaatgagg cgccgcattc cctgcaaatt caatgtctga tcctgaaaaa
    12601 cggacggcaa actggttggt tattgctcgc tattgacaat tcatttcgac agacagacag
    12661 attttttttc attccaaaaa ccgatttaag tcatgcgaaa tgtaattgca gcctttatca
    12721 tcatgatcgt agccgtgatg acggcacaca cacaatctgc cctgcgcccc cgccaattgg
    12781 tggagcaggc acatctcgaa ggcaagtcct tcactccgat ttccctcttc gagcgggcca
    12841 ttaatttccg ctccgaagta gaaataacac ccatagagga ctatacactc ctgaacttgg
    12901 acagcgaagg cttgcgccaa ttggccgccg ccccgcccgc cgcgctcctg ctggaactgc
    12961 catttgaaag cggcaggctt cagttggaac tcgtgagaac cgacgccgac cccatcccgg
    13021 tgcgcgaatc gcagcccacc gattttggtc gcaacagctt caccggactg cactaccggg
    13081 gcgtggtgcg cggtcaggaa ggctccgtgg ctgccgtcag ttttgtggcc ggggaggtca
    13141 tgggcttagt atcctcgcgg caggaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcccgtgt tggaagccat ggaaatggat
    13261 tgtcacactg ccgacgacgg ccccgactac ctgccgcacc agttgcagga ggtcttggag
    13321 ttcagaaacg aggccaaatg cacgcgcatt tattttgagg tggactatga tattttcacc
    13381 gacaagggcg gcacggccgg agccatttct tatgtgaccg cgctgttcaa tcaggtggct
    13441 gcgctgtacg ccaacgaaaa catcggcatt gccatttctg aaattttcgt ctggacgacc
    13501 aacagcccct acatgggcag caatccgggt acgctgctga accagattca gaccttgcgc
    13561 acgcagttca atggccattt ggcgcacctg ttgtcttaca agccctccgg cggcattgcc
    13621 gtgctatcgg gcttgtgcca tccgctgacc attgccaaat tcggcgtttc gggtattcgc
    13681 agcaccttct ccaacgtgcc cacctattct tggtcggtga tggtgatagc gcacgaattg
    13741 gggcaccagt ttggctccaa acacacgcac gcttgcgcct ggaacggcaa caacaccgcc
    13801 atagacggtt gcgccggatt tgtggaaggc agttgcgcgc tgccgcccat gcctgctgtg
    13861 ggaacgggca ccatcatgtc gtattgccat gtcaccagcg taggcatcaa tctgagtctg
    13921 ggtttcggcg cccagccggg caatgtgatg cgcaacgccg tagccgcagc gccttgcctg
    13981 ccggtttgca ctacatcggg cggtggcggc ggaggtggaa accccacgcc cacttgcgcc
    14041 aacaacaccg tgattttgag attggtattg gacaattacg gcccggagac gacctggcgg
    14101 ctcaccaatt cgacaggaca ggtggtgaaa agcggcggcc cgtatcccaa aggtatggcc
    14161 ggcacggcga tactcgacac gatgtgcctg cccaacggct gctatacgtt ccgcattctg
    14221 gacacttatg gcgacgggat ttgctgcaat tacggcaacg gctcttacac actgcgcaat
    14281 gctgccgggc aagtgctgat taccggcggg cagtttggtt ttgaagaaag caagagcatt
    14341 tgtgtcccca atacgccgcc ggctannnnn nnnnnnnnnn nnnacggggt gtgtgcaggt
    14401 caattttgcc caaactcccg ttgtttctta tggcggcccc gaagacctcg gttcgtttac
    14461 actgctgagc agcaacactg tgctgcgcat tcaaaacaat gcctggaaag ccgtgatgct
    14521 gaactacaac gtgacgccca acaccaagtt gcagttcgat tttgcctcca ccgcgcccgg
    14581 cgaaattcac ggcatcgggt tcgacaacga cagcatcatc gggccggaac gcaccttcaa
    14641 actgtacggc agccaaaact ggggcatcac caattttaat aattatcccg gctacaacag
    14701 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcaa
    14761 ccgcctgttt ttcgccgccg accatgacgc atcgccggcc aacggcaact ccttcttccg
    14821 caatatccgc atttacgaag gtacggcgtg cagcaatttg ataccagaca acgaaatgga
    14881 actagccgca ctgttggaag aagaacccgg cggacaacgc gtgcagctct ttcccaaccc
    14941 ggtgagcgac gaactgcaac tgcgcttcac ggctccgctg agcgccgacg aaccggtgat
    15001 actgcgcatt ttcagcgctg ccggccagtt ggtgcaacag gaaaccttgt cttcagccca
    15061 ggccaaacag cccgtgcagg tcgcgcaact gcgttcgggc gcctacctgc tccatattca
    15121 gagcggagca tactctgaga acctgaagtt cataaaacac taatcaacac cggacaaccc
    15181 gtgttttacg agccgcttcc tgcaacagga ggcggctttt tttattaaaa aatcgcagta
    15241 cctgtttagg atggcacatt agcgaggtga catcttccgc ccttttgcaa caaccggagg
    15301 gcggaaggtg taatctcgca actgtgaagg ggggatctcc ctgttttcat gcgacacctc
    15361 cccgtctcgc taaggctcgc ggttagatgt cacctgaaaa catacggccc ccatttcatt
    15421 ttcctacctt tgccgccctg atactatatt gatggtcaaa ttactaccat gaaaaagcaa
    15481 ctttggatac ttctctgctt attgcctttg gcgctgagcg ctcaaaaaaa cattttccag
    15541 tggcaacccg cccgctatga tgcgactgaa acgctggcag ccgagctgga acactgggaa
    15601 atatttcaaa tcaatccttt ggaaatgagc cgcttggccc ggcagtaccc gcaaggcgcg
    15661 gatatgagct ggcaatggcc aaccggagaa gtgactttcc aattgtttgc cgacgatatt
    15721 cgggcaagcg ggtatcgcag cgcggaactg agcgccggcg gggttcggga attgccggca
    15781 ggccccaata tcacttacgg aggccaggtg gccggtggcg gcaatatgcg gctgacactg
    15841 cacgatgatt ttatttttgg atattgggaa aaggatgggc agatctggtt tgtgcagccg
    15901 ctgcgccatt ttgtaccctc ggcgccggag catcattttg tcatttacaa aacttccgat
    15961 gtaaaacccg tgccgaatgc tcactgcggc tggacgcacg ctgagcagca tcgcaaacag
    16021 cacgccgagg atgtacaaca tgcaaaaatg gccggcgaat gctatcaggt ggaaattgcc
    16081 atcgcttccg actttctcat gtttcagttc ttcggtaccg tcggcgctgt gcaggacttt
    16141 accattggcg tgctcaatac cgtgcaaacc aactacgaca acgacttcgc cgacgaaatc
    16201 cgcttcctga ttgtagccaa catggtttcg tcttgcgcta cctgcgacct ggactgcctc
    16261 caccgtgtcc aacgagctgt tggtgtcgtt ccgcgcctgg ggcaacggcg gcggatttgg
    16321 tgtaaattat gacgtcgcct cgctttggag cgatcgggat ttcaacggaa acaccatcgg
    16381 gctggcatgg gtaggcgccc tgtgcagcgg cagcagatac aatattctgg agcgctttac
    16441 cagcaattcg gcattcctgc gcaacctcca ggcgcacgag ttgggtcaca attttgatgc
    16501 cgatcacgac gctaccggct cgcccaccat catggcgccc agcgtaaacg gctccaacaa
    16561 ctggtcggcg ctctcggtca gcgtcattaa caactacatc accttcattg ccggtcaggg
    16621 cgattgttnn nnnnnnnnnn nnnnnnnnnn nnnnccagcc attggctccg acggcccaga
    16681 ttcagttgcc ggttacgcat gtttgccccg gcagcgtggt accgctgatt gacaactccg
    16741 caggcacgcc tacctcctgg aactgggggc tttccggcgc tacgcccccc tcctcttttt
    16801 tgcaaagccc gaccgtgacg tacgacaatc ccggcaccta ccctgtggtg ctgacggtct
    16861 ccaatgccat cggctccgat acgcaatttt ccaacaccga tattgtagtg gatgaaaacg
    16921 gacagaaatt tctgctctac gaaacctttg aaaatcctat actttactgg gatattgtaa
    16981 accccgacaa cggcatcacc tgggatgtgc gcgaagtggg cggctcgcct tacggcaata
    17041 aagcagccta catcaactcg gccggataca gcggctccgg tcaggtggat ggtatgattt
    17101 caccctcgtt cagtctctcc ggcgtgagca atccggtgct gaaaatagac tatgcctacc
    17161 ggcgcagaac cagctccggc agcgaacaac tgcgcgtcaa agtgtccacc aacggcggcg
    17221 ctactttccc caataccatc ttcaccggcc aggaaaacgg cagtggcaac tttgctaccg
    17281 gcggggtgct gggcagtgct ttcacacctg cttccgcctc cgatt
//
LOCUS       DAORLF010000039        17018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1802_length_17018_cov_2.011319, whole genome shotgun sequence.
ACCESSION   DAORLF010000039 DAORLF010000000
VERSION     DAORLF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17018
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_17018_cov_2.011319"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>718)
                     /locus_tag="PK198_05550"
     CDS             complement(125..>718)
                     /locus_tag="PK198_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38227.1"
                     /translation="NGGCLGYPALILMSSMIDTMGSYFRECSLTIPVDSKNESIEKGS
                     DHFLILNQENLFNLGLSKKTIIDFYSTYRSKLVHNSALPPNNFLINDKDSNNIFTLDQ
                     DLKIRCINIYALFEAIKKATEQFLAWLEFGTFSNDHKLTQELLEVAKPPFYDFSKEVQ
                     NQASNPSGCSYDPFEQETGMYIQIKDDDDFDNSLYSK"
     assembly_gap    719..729
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(929..>1542)
                     /locus_tag="PK198_05555"
     CDS             complement(929..>1542)
                     /locus_tag="PK198_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF38228.1"
                     /translation="GYPGESAFNSVISGTASLIDKGFVDKARIGLQGHSWGGYQGAYL
                     ITKTDMFRCAEIGAPVVNMISAYGGIRWESGVVRQFQYEQQQSRIAGTPWQYPLRYLE
                     NSPIFTMDKVNTPVLILHNDKDGAVPWYQGIEMFTALRRLNKPAWLLNYNDEPHWPVK
                     LQNRRDFQRRMQQFFDHYLLDAPMPSWMERGIPAVEKGVTRGY"
     assembly_gap    1543..1679
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1680..>1912)
                     /locus_tag="PK198_05560"
     CDS             complement(<1680..>1912)
                     /locus_tag="PK198_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38229.1"
                     /translation="KDADKWLFTRQNFQTFPDLQYSSDLVSFKQVSNANPQQKDYRWG
                     TIELYEWTAADGQKLQGMLVKPEGFDPKKQYPM"
     assembly_gap    1913..2078
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2079..>3233)
                     /locus_tag="PK198_05565"
     CDS             complement(<2079..>3233)
                     /locus_tag="PK198_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38230.1"
                     /translation="SDSTKTEKKKKESAENGTRFVIRHLATNEDRALQYVTAATMAEK
                     QPVFLLATTGGDSTWLPGVYRFDGADQQLRHIYNGKAKFKHLALDEQGQQAAFLADSD
                     TTKARIRPWDLYCQLPGQDTAALSVRARSEFMPAGWTMSENGALRFSQDGSRLLFGIA
                     PEPFLTDTTLLEEEIVHVEVWKYDEPRLYTQQKVMLDQERKRNYLCLLYPAEHRVVPL
                     ADLDVPDVRLGNENNSSVALGTYEQPYLMETSWTGGPACRDVYLIDAKDGSKLLIHKQ
                     LCSVPQLSPDARYVYWYLRADSAWYAYSVADKQTRRIAGKETANFFNETSDTPALPGP
                     YGIATWGQHDDFALLYDRYDIWQIDPAGLGEPRRMTNGRSEKTVYRYINTD"
     assembly_gap    3234..3251
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3252..>3606)
                     /locus_tag="PK198_05570"
     CDS             complement(<3252..>3606)
                     /locus_tag="PK198_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38231.1"
                     /translation="KPQLSDDGRWTCYELTTLEGDPELMLYDGLQNKEYRFARAQEAG
                     FSADNSYLVFRIKPSVDTLKEQRRRKVKKEDLPKDTLGIYSLSKHALEKIPDLKTIYR
                     PRKWSGWLAWSHEVMK"
     assembly_gap    3607..3621
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3869..4357
                     /locus_tag="PK198_05575"
     CDS             3869..4357
                     /locus_tag="PK198_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38232.1"
                     /translation="MTHTHFRPRSFFLMAMLALLALPVWSQSTPDKQAPMEIVEIFRV
                     VENMPSYPGCEHITDEASKDACTQRKVLEYLKTNMSYRPAVFEKSVDYVAVIQFLVSD
                     TGLVYDMKVTKAPDEESRAALLKSLQSMASRMLFTPGTQRGRAITFIYRMQVDLKKLA
                     GK"
     gene            4463..>6480
                     /gene="gyrA"
                     /locus_tag="PK198_05580"
     CDS             4463..>6480
                     /gene="gyrA"
                     /locus_tag="PK198_05580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRF38233.1"
                     /translation="MSTEERIIPINIEEEMKTAYIDYSMSVIVARALPDVRDGLKPVH
                     RRVLYGMHELGVAFNKPHKKSARIVGEVLGKYHPHGDSSVYDTMVRMAQSWSLRYPLV
                     DGQGNFGSMDGDSPAAMRYTEARLTRIADEILADINKDTVDFALNFDDSLEEPTVMPA
                     KLPNLLVNGASGIAVGMATNMLPHNLREVCDGIIATVDNPDITIDQLTEYIKAPDFPT
                     GGIIYGYSGVKEAFQTGRGRVVVRGRAEIHADDHGRESIIITEIPYQVNKAMLVTKIA
                     ELVEEKRVLGISDIRDESDRNGLRVVVDVKRDAMANVILSQLYKFTPLQSSFGVNNVA
                     LVGGRPMTLNLKDLIEEFIKFRFEVIQRRTRFDLRKALERAHILIGLLIALDYIDAVI
                     DLIRSSATPDEARLGLMRGAFIDDKEKFWKQFSTLIDASQSEDYPVAEGDVLSEAQAK
                     AILEMRLQRLTGLERDKLQQEYDEIQKLIAHLRALLESEALQREVIKTELTEIRDKYG
                     DERRTEINYSDGDISIEEMIKEEEVVITISHLGYIKRTPVGEYRTQSRGGRGSRGAKT
                     RDTDYVEHLFTGNTHSYLLFFTQQGRCFWLKVYELPEASKTSAGRAIQNIIAIPPDDK
                     VKAYVIIKSLDDAEYINNNYIMFCTSNGTIKKTPVEAFSRPRAGGINA"
     assembly_gap    6481..6646
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6647..7046
                     /locus_tag="PK198_05585"
     CDS             <6647..7046
                     /locus_tag="PK198_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRF38234.1"
                     /translation="DRADDAVMGMVCVDPEDKSTSILVISEKGYGKRSEVDDYRLTNR
                     GGKGVKTMQVTDKTGDVMAINAVKDEDDLMITTKSGIVIRMPMDDIRVMGRATQGVRV
                     IRLDNDDDIADITVVPRQDQEEAEHGAAEE"
     gene            7166..8512
                     /locus_tag="PK198_05590"
     CDS             7166..8512
                     /locus_tag="PK198_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38235.1"
                     /translation="MKKLLFIFAIAAFAVSALSAQDGEKGLKDATRNFNTYKLDPSNN
                     KSKLTEARTAIDIAANDAVTAALLKTWQLRGDIYNEIATQKINATQLAAQGLPTGDMS
                     ALDAIEHPGIEAAKSFTKALEMAVKKFEIKDALNGMKALQNNLQNLGIMQYEKQDFKS
                     AFHSFAACLDMHELLAKNKEESMFSDPAKYEDQLFYVGLAGASANMSAESKPYFLKLY
                     EMKAQKPAIYEALYSIEAAQTSPEAAYKYLDEGRKLFPDEVSLLFAEINHYLRIGKLD
                     ELIGKLEAAIKVEPNNVSLYTTMGSVYDNLYQKEFAAGNKDKAEGYAAKALDYFNQAL
                     AKDEKNFDALYSIGALYYNKAASMTAELNKLADDYSKAGIEKYKAKQKEVLGQFDLAL
                     PYFQRCEKSNPNDQNTLIALKEIYAKKDELDLSNEFKARLEKVNAGEKNASSYFKN"
     gene            8633..9325
                     /locus_tag="PK198_05595"
     CDS             8633..9325
                     /locus_tag="PK198_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38236.1"
                     /translation="MRYLFLLSLFTIFALQANAQLGVGGAYLTSSARHWEIRTAGATN
                     LRLQPPGTGYSVGLDYQFRLKKVRIEFAPELNYAHWSTPETSELETNSNWYSFFFNVR
                     VYPFDLKGDCNCPTFSKRGNTFQKGLFVSLSPGITQMNYNIPRGWGGVSFNMKDQSWA
                     PNAALILGLDLGISELLTVTPHAGWRYFPQAEWPSLATVVAQSGILNVDSEGPLHQFH
                     VGLRLGWRLGRR"
     gene            9476..>10757
                     /locus_tag="PK198_05600"
     CDS             9476..>10757
                     /locus_tag="PK198_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38237.1"
                     /translation="MAKRFVVALSVSLWLSVSLQAQKDCRLEFAAELPTEPCRDYREY
                     APLEASHTPVKLIRIVVHVIQQDAGLPARNFENNRTGNDWINAMLWQLNVIFRNLVPI
                     SHPIADCPSTYMKDARIQFRTDSIFFHQNSRHWKHGSRGGTTWCNIWDDLIRDNPAIP
                     NDLKDNAFHVFLVHSTWSEWHTGFSPGIGILYENFVVMGGYYIALFEKDKPDLDNTPY
                     GAALTLAHEFGHAFGLYHSNGGDYCCDTETGKTNNLMNSGAAALSECQLARMHYLLES
                     GEHATQKGQSSGICKAIATDYCHKDESYDIEIPASQTVIWSASKKLTTDIIVRSGAQL
                     IIRCRIGLPDEARIEVERGARLIIDGGSITHNHALWPRCESGVWGGIVAQGGIKRITK
                     KMELHSPTLPKPSQAGEAWLRNAVLEHVAGTGILR"
     assembly_gap    10758..10837
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10838..11277)
                     /locus_tag="PK198_05605"
     CDS             complement(<10838..11277)
                     /locus_tag="PK198_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38238.1"
                     /translation="MQFSTVSIIKHPLELVWDAMQYRLPEIAMQVDDIAEIAGLEHLI
                     TETGHNKVINVWKAAPPLPDFLKNMVQPDMLTWTDSAEWNPDSRNCFWNIESHYFREK
                     MICSGVTTYHPALGGKGCRLSFEGELKWHAGALNSIVMQGMDTVL"
     gene            complement(11306..12346)
                     /locus_tag="PK198_05610"
     CDS             complement(11306..12346)
                     /locus_tag="PK198_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF38239.1"
                     /translation="MEVLVTGANGFIGSHLCRALMAKGYRVRALVRPGSDLRSLAGLE
                     LTYCYGDILQPDSLAQAAAGCSLLFHVAGVFAYSGVKGEQLISEARSGAAHVVQAAVQ
                     AGVKRMVLTSSSVTYGATNQRLALDETHPGGFQDASDYVTSKRIQEETAFQLAAQTGL
                     DLVSVHPTLTVGGPDYGLTESNHAIVSYLNDPYKTTWIGGCNIVSVKDIAEGHILVAE
                     KGLRGERYLLGAENLDWSEVHRNISELCGLPGPYLTANHAAAYLAAAFHEALALISRQ
                     RPPSTREQARMAGNYYWYDHSKAARLGYAPMTAREALASAVEWLITSDHVSASLRSAM
                     QLHEDVYKMRRK"
     gene            complement(12353..>13182)
                     /locus_tag="PK198_05615"
     CDS             complement(12353..>13182)
                     /locus_tag="PK198_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38240.1"
                     /translation="RRYIDGSIIFEYIRIIPVDYEDFVNRVDISQAVQMMYDNIGGAR
                     VVVKSDDQNRTMYQAERDLYLPQPNWMILFGGKHIDVDKLEVIRYNEHSCRNFWRTVH
                     SANQSAEYDDGMVAFEKDPGGTRVTIVARQKFALPLFWQLFDIDYLPDVKEALVSDAY
                     SRFFSRTMANFEAAAEGRVPFIGKAWDTRYAEHAHEGIPPEIEHLKYLFSMLAGMAEK
                     WVRKDNAPAGATALGTDELGFRHFASPGKADEVSEGVSRFFSELAAAIGKDIQLLGK"
     assembly_gap    13183..13223
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13224..13489)
                     /locus_tag="PK198_05620"
     CDS             complement(<13224..13489)
                     /locus_tag="PK198_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38241.1"
                     /translation="MFPFKNMVDGRLNAILAHRLKDVDDNSAARWGLTAINYALANLE
                     QFRQGWQVLFDKDSLHRIDTRLNVDIDKLSDADFESIEGYILPF"
     assembly_gap    13561..13599
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13600..14488)
                     /locus_tag="PK198_05625"
     CDS             complement(<13600..14488)
                     /locus_tag="PK198_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRF38242.1"
                     /translation="MQTLPTSTASARVCIVQNEQRNPADLLAEVLQQAGFEDAIERKR
                     AMLNMGKSYFCILIKPDMELFDHRSNTGTDPLLVEELIDMLHEKGYTNVAVADAEGSA
                     SLWLDNREVLVLADLAGYRFVTSKGNAYDVLNLSEATIDAEFPAGSALAGTELSEYWL
                     SAHFRINFCKNKTDEAHGFALGLYNLMGILPLKAKSFHYKLRLKQEEVALDLYRHAPV
                     DFAIIDAWCSNHGSQGSRCTRPLQTCAFIASESLLLADWVGALKMGLDPYLSPLNAAV
                     LRACGLPGNYQMEGDLRPWP"
     gene            complement(14501..15736)
                     /locus_tag="PK198_05630"
     CDS             complement(14501..15736)
                     /locus_tag="PK198_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38243.1"
                     /translation="MKKYPYAFHIALDGNGINGIEGMAGVCCFLFDPADNSFAFKINY
                     YNGLAGGHALHISPGGTYGYLGTTGQHLMLYDPVSLNELSRQTTLLYEKNDTSIRGST
                     HVAWMNDKEFIAAIGDYFYQFHVDQLDKGRKLGPHKIMLPHSMKFTASRRYLCYGSMD
                     NPALGKNGEAKMVGIWDMETGEATRIDLPATCWHLVPHPTEDLFYAVSFRVLPQDHVD
                     YHQWAMAFFKEYAFEIDAKEKRVRRHWACGREIPAHINSDVTISDTELIFCNGGSHTI
                     VFIDLKSFSDFRMIDELPSLSEAMNHKRTMATTVYDSLVRGGFYTANQHLLGALRISR
                     FRLMDSVHACQLSKDQSLLFTANRGLNHITIYDYPSLELRLRVPMPDIQEFVPYVGRL
                     GDPRLGFHHSYLVSPGGEK"
     gene            complement(15754..16485)
                     /locus_tag="PK198_05635"
     CDS             complement(15754..16485)
                     /locus_tag="PK198_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38244.1"
                     /translation="MAEQSTNGDLVKDFERVVKKAIEANTTYYKEAAAALQNLYSGKT
                     ELSGSNVMESLSEAWTSLVKLQLRYAENLYDLQLHWSKNMAHQPAPAAAPQQTNIEPV
                     PERKVMTLTGESGQSLEFLLHFNSRDAETRHCKFILSGFVDAANGAAAPLLLVCEPES
                     FVLIPGQTTEVRFSVNIPETTGPGAYRNTVTVAGYDDSIFDLIVAVQPPAEKADTPPV
                     EKMPGSDKRSSSVIGAKPKTPRKTS"
     gene            complement(16516..>17018)
                     /locus_tag="PK198_05640"
     CDS             complement(16516..>17018)
                     /locus_tag="PK198_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38245.1"
                     /translation="SDCCRPGGVCPPQPDCPPRCLAEIKRSACPGEVMVVPFNVKNTC
                     GNARTYRIGIRPMVNTQGEPAPVQPWLDRQELHLEPGQTATVSLTLSLIEGFRAGDCY
                     EAEIVIRENKVNQNVCFQLCVTACQPAVEVRPLDEKRYFMRWQSWQDHFYCEQAPDRS
                     PNRTQG"
BASE COUNT         3961 a         4371 c         4123 g         3890 t
ORIGIN      
        1 cgcgagaaaa accgcagggt ggatgtgcga gttacagtca tggagtaggg gcatgctgtt
       61 ccaaatgcca cagcgttgca cttcgcggca acactaaaac ccagtcctct ctctgtccta
      121 cacgctattt cgagtatagg gaattatcaa aatcatcatc atcctttatt tggatataca
      181 tgcctgtttc ctgttcaaaa ggatcatacg aacatccgct tggattagat gcctgatttt
      241 gcacctcttt cgagaaatcg tagaaaggag gtttagcaac ctctaaaagc tcttgagtca
      301 atttgtgatc attgctaaag gttccaaact ccagccaggc taaaaattgt tcggtagctt
      361 ttttaattgc ttcaaaaagg gcatatatat tgatacatcg aattttgagg tcttgatcaa
      421 gtgtaaagat attatttgaa tccttatcat taatgaggaa attattggga ggcaatgcac
      481 tattatgtac tagttttgag cgataagtac tgtaaaaatc aatgattgtt tttttgctca
      541 gtcccaaatt gaatagattt tcctgattta aaataaggaa atgatcggat cctttttcaa
      601 ttgattcatt ttttgaatct acgggtatgg tcaaactgca ttctcgaaaa tatgatccca
      661 ttgtatcaat catagatgac atcaaaatga gagcaggata cccaaggcaa ccaccattnn
      721 nnnnnnnnng cccaatcaga tgatgatttt ttatcaaaat caaggcagta ctttatcgtt
      781 actaacagct ctgttaacgc ctccctttgt tgttctcttt gtattcgatt cattttctga
      841 aatgttgtta atgaaaaact acaaaccatt atggctgctg aacgctgaag acgatgacct
      901 tacatactct cccctcactc actcacccct aataccccct cgtcacacct ttttccaccg
      961 cgggaatgcc ccgttccatc cacgacggca tgggcgcatc cagcaggtag tggtcgaaaa
     1021 actgctgcat ccggcgttgg aaatcgcggc gattttgcag tttcaccggc caatgcggct
     1081 cgtcgttgta gttcagcagc caggcaggtt tgttgagcct gcgcaaggcc gtaaacatct
     1141 caattccctg ataccaaggc acggcgccgt ctttatcgtt gtgcaaaatc agcactggcg
     1201 tgttcacttt atccatcgtg aaaatgggcg aattttcgag gtagcgcagc ggatattgcc
     1261 agggcgtacc ggcaatgcgg ctttgctgct gctcgtattg gaactgccgt accacacccg
     1321 attcccaacg aatgccgcca tacgcagaaa tcatgttcac caccggcgca ccgatctccg
     1381 cacaacggaa catatcggtt ttcgtgatga gatacgcccc ctgatagccg ccccagctat
     1441 gcccctgcaa accgatgcgg gccttgtcca caaagccttt gtctatgagc gaggccgtgc
     1501 cggaaatgac cgaattgaaa gcgctctcgc cgggatagcc ctnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1681 atcgggtatt gtttcttcgg atcaaagccc tccggtttta ccagcatccc ctgcaatttt
     1741 tgtccgtcgg cggcagtcca ttcgtacagt tcaatcgtac cccagcggta gtctttttgc
     1801 tgcggattgg cattgctcac ttgtttaaaa ctcacgagat cgctgctgta ctgcaagtca
     1861 gggaaggtct ggaaattctg gcgggtgaac agccatttgt cggcatcctt tgnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cggtattgat gtagcgatag
     2101 acggtttttt cgctgcgtcc gttcgtcata cggcgcggtt ctccaagtcc ggcggggtct
     2161 atttgccaaa tatcgtagcg gtcgtacagc aacgcaaaat cgtcgtgttg gccccaagtc
     2221 gcgatgccgt acgggccggg caatgcgggc gtatcgctgg tttcgttgaa gaagttggcc
     2281 gtttccttac cggcgatgcg gcgcgtttgt ttgtcggcca cagagtaggc ataccaagct
     2341 gagtcggcgc gcaggtacca atacacatag cgggcatcgg gcgaaagctg cggcacagaa
     2401 cacaattgct tgtgaataag gagtttactg ccgtctttgg cgtctatcag atacacgtcg
     2461 cggcaggccg gtccgccggt ccagctcgtt tccatcaggt aaggctgttc gtaagttccc
     2521 aaagccaccg atgaattgtt ttcattgccg aggcgcacat ccggcacatc caggtcggcc
     2581 aatgggacta cccggtgctc ggcgggatac agcaggcaga ggtagttgcg cttgcgttcc
     2641 tgatccagca ttaccttttg ttgggtataa agtcgcggct catcgtattt ccatacttcc
     2701 acatgcacaa tttcctcctc cagcaaggtg gtatcggtca gaaatggctc aggtgcaatg
     2761 ccaaacaaca ggcgactgcc gtcttgcgag aagcggaggg cgccgttttc gctcatcgtc
     2821 cagccggcgg gcataaactc cgaacgcgca cgcacactga gggcggcggt gtcttgtccc
     2881 ggcaattgac aatacaaatc ccagggccga atgcgggctt tggtggtatc gctgtcggcc
     2941 agaaaagccg cctgttgacc ctgctcgtcg agcgcgaggt gtttgaattt ggctttgccg
     3001 ttataaatat ggcggagttg ttggtcggct ccgtcgaagc gatagacgcc cggcagccag
     3061 gtggagtcgc cgccggtagt agccaagagg aaaacgggct gtttttcggc catcgttgcg
     3121 gcggttacat attgcaaagc cctgtcttca ttggtagcca aatgcctgat aacgaagcgc
     3181 gtgccgtttt cggcgctctc cttttttttc ttttcggttt tggtactgtc gctnnnnnnn
     3241 nnnnnnnnnn ntttcatcac ctcgtgcgac catgccagcc agccgctcca tttgcgcggc
     3301 cgatagatgg ttttgagatc ggggattttc tccaaggcgt gcttgctcag gctgtaaatg
     3361 cccagcgtgt ctttgggcag gtcttctttt ttgaccttcc ggcggcgctg ttctttgagc
     3421 gtatccactg agggtttgat gcgaaaaacc agatagctgt tgtcggcgct gaagccggcc
     3481 tcctgcgccc tggcaaaacg gtattcttta ttttggaggc catcgtaaag catcaattcg
     3541 gggtcgccct ccaaggtagt cagttcataa caggtccatc gtccgtcgtc ggaaagctgt
     3601 ggttttnnnn nnnnnnnnnn ncttttccaa tggtcgaaat cggcatgatc gagcgttttt
     3661 ttggcaacgg gctggaccgt cggggtggaa acgcgtgttt ttttggcggc gggctgggct
     3721 tgcagacctg aagcgagcag catagcggcc cacacgaagt agagataccg gtgcatatcg
     3781 ggtgatattt gttgtgcggc aaatgtaaag aaaaaatgag gttttcatat cttcgcagct
     3841 ttcttcacca attcaaactt tgctctttat gacacacacg cactttcgtc cccgttcctt
     3901 ttttcttatg gctatgttgg ctttgttggc gctgccggtt tggtcgcagt caacgccaga
     3961 taaacaagcg cctatggaaa ttgtggaaat attcagggtc gtagaaaaca tgccttccta
     4021 ccccggctgc gagcatatta ccgacgaagc ctccaaagat gcctgcacgc agcgaaaagt
     4081 cctggagtat ctcaaaacca atatgtctta ccggcctgcc gtatttgaaa aaagtgtaga
     4141 ttatgtggcg gtgattcagt tcttagtttc cgatacgggg cttgtttacg atatgaaggt
     4201 caccaaagct cctgatgagg aaagccgggc cgccctgctc aaatctctgc aatctatggc
     4261 ctcccgaatg ctctttacac cgggcacaca acgaggccgg gcgatcactt tcatttacag
     4321 aatgcaggtg gatttgaaaa agttggccgg gaaataatca aaccacattt agaccacatt
     4381 cccggcacaa aaaccgacca aattttacta cctttgcacc ccggaaaacg aggggttcgt
     4441 tttgaaaacg atttgaaaat ttatgtcaac cgaagaaagg attatcccga ttaatatcga
     4501 ggaggagatg aaaaccgcct acattgacta ctccatgtcg gtcattgtag cccgcgccct
     4561 gcccgatgtc agagacggcc tcaaacccgt tcaccgccgg gtgctatacg gaatgcacga
     4621 attgggcgta gcgttcaata agcctcacaa gaagtcggcc cgtatcgtag gggaagtgct
     4681 gggtaagtac cacccccacg gcgactcatc cgtttatgac acgatggtgc gcatggccca
     4741 aagctggtcg ttgcgctacc ccttggtgga cggacagggc aacttcggct ctatggacgg
     4801 cgacagccct gcagccatgc gttacaccga ggcccgtctg acccgcattg ccgacgaaat
     4861 cctggcagac atcaacaaag ataccgttga ttttgcgctc aacttcgacg attcgctcga
     4921 agaacccacc gtgatgcctg ccaaattgcc caacctgttg gtcaacggag catcgggcat
     4981 tgcagtgggt atggctacca atatgttgcc gcacaacctc cgcgaggtct gcgacggcat
     5041 aattgccacc gttgacaacc cggacatcac catagaccaa ctcaccgagt acatcaaagc
     5101 gcccgatttc cccaccggcg gcatcatcta cggctattcc ggcgtgaaag aagcctttca
     5161 gaccgggcgc ggcagagtag tggtgcgcgg cagagcggaa attcatgccg acgatcacgg
     5221 gcgggagtct atcatcatca cagagattcc gtaccaggtc aacaaggcca tgctggtgac
     5281 taaaatcgcc gaattggtag aggaaaaacg cgtcctcggc atcagcgata ttcgcgacga
     5341 atccgaccgc aacggcctgc gcgtggtggt ggacgtgaaa cgcgacgcca tggccaacgt
     5401 cattctcagc cagttgtaca agtttacgcc gctgcaatcc tctttcggcg tcaacaacgt
     5461 agcattggta ggcggccgcc ccatgacgct gaatctcaaa gacctcatcg aggagttcat
     5521 caaattccga ttcgaagtca ttcagcggcg cacccgtttc gatctgagaa aagcattaga
     5581 gcgggcacat atcctcatcg gcctgctcat tgctttggat tacattgacg ctgtcattga
     5641 tctcattcgc tcatcggcca cgcccgacga agcccgcctc ggcctcatgc gcggcgcctt
     5701 tattgacgac aaggagaagt tctggaagca attttcgacg ctcattgatg cctcgcaatc
     5761 agaagattac cccgtagcag agggcgacgt cctgtcggaa gcgcaggcca aggccattct
     5821 ggaaatgcgc ctccaacggc tcaccggctt agagcgcgac aagctgcaac aggagtatga
     5881 tgaaattcaa aagctcattg cacacctgcg cgccctgttg gaaagcgagg cattgcagcg
     5941 cgaggttatt aaaaccgaac tgaccgaaat ccgcgacaaa tacggagacg agcgccgcac
     6001 ggaaatcaac tactccgacg gcgacatcag catcgaagag atgatcaaag aggaggaagt
     6061 ggtcattacc atctcgcacc tcggatacat caaacgaacg ccggtaggcg aataccgcac
     6121 ccaaagccgg ggcggacgcg gatcgcgcgg ggccaaaacc cgcgataccg actatgtgga
     6181 acacctcttt accggcaata cgcacagcta cctcctgttc tttacgcagc aggggcgctg
     6241 tttctggctc aaggtgtatg aactgcccga agcttctaaa acctctgccg gtcgggccat
     6301 ccaaaacatc atcgccattc cgcccgacga caaagtgaaa gcttatgtca tcattaagag
     6361 tttggacgac gcggagtaca tcaacaacaa ttatatcatg ttctgtactt ccaacggcac
     6421 catcaaaaaa acgccggtgg aagctttctc ccgtcctcgc gcaggaggca tcaacgccat
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagac agagcagacg
     6661 atgcagtaat gggtatggtt tgcgtggacc cggaagacaa gagtaccagc attttggtta
     6721 tttctgaaaa aggctacggc aagcgctcgg aggtggacga ctaccgcctc accaatcggg
     6781 gcggcaaggg ggtaaaaacc atgcaggtca ccgacaaaac cggcgacgtg atggccatca
     6841 atgccgtcaa ggatgaagac gatctgatga ttaccaccaa atccggcatc gtcattcgta
     6901 tgcccatgga cgacatccgc gtgatgggcc gcgctacgca gggcgttcgc gttattcgtt
     6961 tagacaatga cgacgacatc gccgacatta ccgtggttcc gcgacaagat caggaagagg
     7021 ccgagcatgg cgccgccgaa gagtaaaacc gatacgaacg ccgataaaaa agcgttttca
     7081 tcgcagaagt ttaacatttc aacaacttac cgacggcctc aacagccgtc tcaaacttac
     7141 attcacaaat aaaatctgca ttctaatgaa aaaactgctt ttcatcttcg ctatcgccgc
     7201 cttcgcagtt tcggctttgt ctgctcaaga cggtgaaaaa ggtttgaaag atgccacccg
     7261 caatttcaac acctataagt tggacccatc caacaataaa agcaaactaa cagaagcccg
     7321 cacagccatt gacattgctg ccaatgatgc agtaacagcc gctctgttaa aaacctggca
     7381 attgcgcggc gatatttaca acgagatcgc cactcagaaa atcaatgcca ctcaactggc
     7441 cgcacaggga ttgcccaccg gcgacatgtc agcccttgac gctattgagc atcccggcat
     7501 tgaagcagcc aagtctttta ctaaagcgct ggaaatggca gtgaaaaaat ttgaaataaa
     7561 agatgcgctc aacggcatga aagctctgca gaacaacctg caaaacctcg gcatcatgca
     7621 atatgaaaag caggatttca aatctgcgtt ccattcattc gcagcctgct tagacatgca
     7681 cgaacttttg gctaaaaaca aagaagagag catgttcagc gatccggcca agtatgaaga
     7741 ccagcttttt tatgtaggtc tggccggcgc cagtgccaat atgagtgctg agtcgaagcc
     7801 ttacttcttg aagttgtatg aaatgaaggc tcaaaagccc gctatttacg aagcattgta
     7861 ctctattgaa gccgcacaga cttcgcccga agctgcctat aagtacctcg acgaaggtcg
     7921 caaattgttc ccggatgaag tttccctgtt gtttgccgag atcaaccact acctccgcat
     7981 cggcaaattg gacgaactta tcggcaaatt ggaagcggct atcaaggtgg agcccaacaa
     8041 cgtatcgctc tacaccacta tgggcagcgt ttatgacaac ctgtaccaga aagaatttgc
     8101 cgccggcaat aaagacaaag cggaaggcta tgctgccaaa gcgcttgatt atttcaacca
     8161 ggccttggcc aaagatgaaa agaacttcga cgcgctgtac agcatcggcg ctttgtacta
     8221 caacaaggcc gcatccatga cagcagagtt gaacaaactc gccgacgact actccaaagc
     8281 cggcatcgag aaatataagg ccaaacaaaa agaggtactg ggccagttcg acctcgcgct
     8341 gccttacttc cagcgctgtg agaaatcgaa ccccaacgac cagaacacgc tcatcgcctt
     8401 gaaggagatt tatgccaaaa aagacgaatt ggatttgtcc aacgagttca aagcacgttt
     8461 ggaaaaagta aatgcaggag agaaaaatgc ctcctcatac ttcaaaaact aataagatat
     8521 cgggggtgct atccgcacct tgaaacaaga ggctgtgtcg gtttcggcgc agcctctttt
     8581 gtttttaaga aaatgcgccc ggtcactttg cgtttaagat aaaaacatca acatgcggta
     8641 cctcttctta ctctcgctgt ttacgatttt tgcgctgcaa gccaacgcgc aattgggcgt
     8701 gggcggcgct tacctgacct cctcggcccg gcactgggaa atacgcactg cgggcgcaac
     8761 caacctccgc ttacagccgc ccgggaccgg ctactctgtc ggtttagatt accaattccg
     8821 actcaaaaaa gtgcgcattg agttcgcgcc tgaactcaac tacgcgcact ggagtactcc
     8881 ggaaacaagc gaactggaaa ccaacagcaa ctggtacagc ttctttttca atgtccgcgt
     8941 ttatcctttc gatctgaaag gcgactgcaa ctgccccact ttctccaaac gaggcaatac
     9001 atttcaaaaa ggcttgtttg tttcgctgtc gccgggcatt actcagatga attacaacat
     9061 cccccgcggc tggggcggcg tttctttcaa tatgaaggat caatcctggg cgcctaatgc
     9121 ggctctcatt ctgggtctcg atctcggcat ttccgaactg ctcaccgtga cgcctcacgc
     9181 cggctggcgg tatttcccac aggcggagtg gccgtcttta gctacagttg ttgctcaatc
     9241 aggtatcctg aatgtagatt ccgaagggcc gctgcatcag tttcatgtgg gtttgcggct
     9301 gggatggcgg ctggggcggc gttagcaatc attaagtagt ggggcaaaat aaatattcat
     9361 tttctttagg ccaaaggatt cgctaagaca cttaaaatga agcgtttact tagcgaatcc
     9421 tttggcaaaa atgaagattt attttttctc gccccttata aacaatggca tatcaatggc
     9481 taaacgtttt gtcgtagctc tttcggttag tttgtggctg tctgtgtcat tgcaggcaca
     9541 aaaggattgc cggctggaat ttgccgccga attgcccacc gaaccctgcc gcgactaccg
     9601 tgaatacgct cccttggagg cttctcacac gcctgtgaaa ctcatccgta tagtggttca
     9661 tgtcatccag caggatgcgg ggctgccggc acgcaatttt gaaaacaacc gtaccggtaa
     9721 cgactggatc aatgccatgc tgtggcaact caatgtcatc tttcgcaacc tcgtgcccat
     9781 cagccatcct attgccgatt gcccctcgac gtacatgaaa gacgcgcgca ttcagtttcg
     9841 caccgacagc attttttttc atcaaaacag ccgccattgg aaacacggtt cacggggcgg
     9901 cactacctgg tgcaatatat gggacgattt gatacgcgac aacccagcca ttcccaacga
     9961 cctcaaagac aatgccttcc acgtttttct ggtacattct acctggagcg agtggcacac
    10021 gggcttctct cccggcatcg gcattttgta tgaaaatttt gtggtaatgg gcggctacta
    10081 cattgccctg tttgaaaaag acaagcccga tttagacaac acgccctatg gcgccgcact
    10141 cactctggcg cacgaatttg gccatgcctt cggcctttac cacagcaatg gcggcgatta
    10201 ttgctgcgac accgaaaccg gcaaaaccaa caacctcatg aattccgggg cggcggcgct
    10261 cagcgagtgc cagttggccc gtatgcacta cctgctggaa tcgggcgaac atgccactca
    10321 aaaagggcaa agctccggca tttgtaaagc cattgccacc gactactgcc acaaggacga
    10381 aagctacgac attgaaatcc ctgcgagcca aaccgtcatc tggtctgcct ccaaaaaact
    10441 gaccaccgat attatagtga gatccggtgc gcagctcatc attcgttgtc gcatcgggct
    10501 gcccgacgag gcccggattg aggtggagcg cggcgcccgg ctcatcatag acggcggcag
    10561 tatcacgcac aatcacgccc tgtggcctcg ttgcgagtca ggagtttggg gcggaatcgt
    10621 ggcacaaggc ggcatcaaac gcattacaaa gaaaatggag ctgcactccc ctaccctgcc
    10681 gaaaccgtca caagccggcg aggcgtggct ccgcaacgct gttctggagc acgttgcagg
    10741 caccggtatt ctaagaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt acagtatcca taccttgcat
    10861 cacaatgctg ttcaaagctc ctgcatgcca tttcagttcg ccttcaaaac tgagcctgca
    10921 ccccttacca cccagtgccg gatgataagt agtgacgccg gagcagatca ttttttctct
    10981 aaaataatgc gattctatat tccagaaaca gttgcggcta tcgggattcc attcggcgga
    11041 atccgtccaa gtgagcatat ccggctgaac catgtttttc agaaaatcgg gcaaaggcgg
    11101 cgcggctttc cacacgttga tgactttatt gtggccagtc tccgtgatga gatgttccaa
    11161 acccgctatt tctgctatgt cgtccacttg catggcaatt tcaggcagcc tgtattgcat
    11221 ggcatcccat accaattcaa gcggatgttt tatgatcgaa actgtggaaa attgcattga
    11281 ttttcagtat cttataaatt gatgttcatt tgcgcctcat cttgtacaca tcctcatgca
    11341 attgcatggc agatcgcaaa gatgctgaaa catgatcaga tgttatgagc cattctaccg
    11401 cagaagcaag cgcctcgcgg gctgtcatag gggcataccc caatctggct gccttgctgt
    11461 gatcgtacca gtagtaattg ccggccatac gggcctgttc tctggtggaa ggcggacgct
    11521 gccgactgat gagcgccagt gcttcgtgaa aggccgccgc cagatatgcc gccgcgtggt
    11581 tggcggtgag atacggcccc ggcagtccgc acaattcgga aatattgcga tgcacctccg
    11641 accaatcgag gttttcggcc cccagcaggt aacgctcgcc gcgcagccct ttttcggcta
    11701 cgaggatgtg cccttcggca atgtctttca cagaaacgat gttgcagccg cctatccagg
    11761 tggttttgta gggatcattg aggtagctga caatggcgtg gttgctctcg gtgagtccgt
    11821 agtccggtcc gcctaccgtc agggtgggat gcacgctcac caaatcaagt cccgtctgag
    11881 cagcgagttg gaaagccgtt tcttcctgaa ttcgttttga agttacataa tccgatgcgt
    11941 cctgaaagcc gccgggatga gtctcatcaa gggcaagtcg ctgattggta gcgccataag
    12001 taaccgacga agaagtgagt accatgcgct tcacccctgc ctgcacggca gcctgcacca
    12061 catgcgcggc gcccgatctg gcctcggaga tgagctgctc gcctttcacg ccggaatagg
    12121 caaatacacc cgccacatga aaaagcaaac tacaacctgc tgcggcctgc gccaatgaat
    12181 ccggctgcaa aatatcgccg tagcaataag tcagctccaa acccgcgagg ctgcgcaagt
    12241 cggaacctgg ccgcaccaat gcccgcacgc gataaccttt ggccatcaaa gcgcgacata
    12301 agtgactgcc gatgaatccg ttggctccgg tgacgagaac ttccatggct gtttatttac
    12361 ccaataattg tatgtcttta cctatggccg ctgccagctc gctgaaaaac cggctcactc
    12421 cttcagatac ttcatctgcc ttgcccggcg atgcaaaatg cctgaatccc aactcatcgg
    12481 ttcccaaagc cgtggcgcct gccggggcat tgtctttgcg cacccatttt tcagccatgc
    12541 cggccagcat cgaaaacagg tatttcaggt gctcaatttc aggtggaatg ccctcgtgag
    12601 catgctcggc ataacgagta tcccaagcct tgccgatgaa gggtacgcgc ccctcggccg
    12661 ccgcctcaaa attagccata gtgcgggaaa agaacctgct gtaagcgtct gataccaagg
    12721 cttctttaac atcgggcagg tagtctatat caaatagctg ccaaaaaagc ggcagtgcaa
    12781 atttttgtcg cgccacgatg gtcacgcggg ttccgcccgg gtctttttca aaggccacca
    12841 taccatcgtc gtattcagcc gattggttgg cgctgtgtac ggtgcgccag aaatttcggc
    12901 aactatgttc gttgtatcga atcacctcca atttgtccac atcaatgtgt ttgccgccaa
    12961 acaatatcat ccaattgggc tgcggtaggt agaggtcgcg ttctgcctga tacattgttc
    13021 tgttttggtc gtcgcttttg acgaccaccc ttgcgccgcc gatgttgtcg tacatcattt
    13081 gtacggcttg tgaaatgtcc actcgattta caaagtcttc ataatcaaca ggaataatgc
    13141 ggatgtattc aaaaataatg gaaccgtcaa tgtaacgacg gcnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnntgaaacg gcagtatgta cccttcaatg gattcaaaat
    13261 cggcatcgga gagtttgtct atatccacgt tcagccgggt atctattctg tgcaggctgt
    13321 ctttatcgaa gagcacctgc catccctgac gaaactgttc gagattggcc agcgcatagt
    13381 tgatggccgt aagaccccag cgggcggcgg aattgtcgtc cacatctttc aaacgatggg
    13441 ccagaatggc gttgaggcga ccgtccacca tgtttttgaa ggggaacaaa tcagtgttta
    13501 ccgtttggag gcaggcttca gcgaggcgct gtgtgagcgg cgacgcattc cgtttagagg
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gggccagggc ctgaggtcgc
    13621 cttccatctg ataattgccg ggaaggccgc aagctctgag tacggcagcg ttcagcggcg
    13681 agaggtaagg atcgaggccc attttcagcg cgcccaccca atcggccagc aacagcgatt
    13741 cggaagcaat aaacgcacag gtctgcaatg gccgggtgca acggctgccc tgactgccgt
    13801 gattgctgca ccaggcatca atgatcgcaa aatcaacagg agcatgacgg tacaaatcga
    13861 gcgcgacttc ctcctgcttc agtctcagct tgtaatgaaa ggacttggcc ttgagtggca
    13921 aaatccccat caggttgtac agtcccaatg caaatccgtg cgcctcgtcg gttttatttt
    13981 tgcaaaaatt gatgcgaaaa tgagccgaca gccagtactc cgacagctcg gttccggcca
    14041 aagcgctgcc cgccggaaac tccgcgtcta tcgttgcttc actcaggttg agcacatcgt
    14101 aggcattgcc ttttgaggta acgaagcgat accccgccag atcggccagc actaatactt
    14161 cgcggttgtc caaccagaga gaggcgctgc cttccgcatc ggcgacagcc acattggtat
    14221 agcctttttc atgcagcata tctatcaact cctccaccaa cagcggatcg gtgccggtat
    14281 tggagcgatg gtcgaacagc tccatatcgg gtttgatgag aatgcaaaaa taggatttgc
    14341 ccatgttcaa catggcgcgt ttgcgttcta ttgcatcctc aaaaccagcc tgttgcaaca
    14401 cttcagccag caagtcggcc gggttgcgct gctcgttttg cacgatgcac acacgagcag
    14461 aagcggtgga tgttggtagt gtttgcattt ccggcattgt tcatttttcg cctcccggac
    14521 tcaccagata gctgtgatga aaaccaagcc gaggatcgcc caagcggccc acatacggca
    14581 caaattcctg tatatccggc atcggaacgc gaagtctcag ctccagcgac ggataatcat
    14641 atatggtgat gtggttcaag ccgcgattgg ccgtaaaaag caagctctgg tctttcgaca
    14701 actggcaggc atgcaccgaa tccatgaggc ggaaacggct gatacgcaaa gctccgagca
    14761 gatgttgatt ggctgtgtaa aaaccgccgc gcaccaaaga atcatataca gtagtggcca
    14821 tggtgcgttt gtgattcatg gcctcgctca gactgggcag ttcgtctatc atgcgaaaat
    14881 ctgaaaaact tttcaaatca atgaatacga tggtatggct gccgccgttg caaaaaatga
    14941 gttccgtgtc tgaaatcgtc acatcggaat tgatgtgcgc cggaatttcg cggccacagg
    15001 cccagtgcct cctcacgcgc ttttctttgg cgtctatttc aaaggcatat tctttaaaaa
    15061 acgccattgc ccactgatgg taatccacat gatcctgcgg taagaccctg aaagacactg
    15121 cataaaacaa gtcctcagtg ggatgcggca ccaaatgcca gcaggtagcg ggcaggtcaa
    15181 tccgggtcgc ttcgccggtt tccatatccc agattccgac cattttggct tcgccgtttt
    15241 tgcccagtgc agggttgtcc atcgagccat aacacaagta tcggcgcgat gccgtgaact
    15301 tcatgctgtg cggcaacatg attttgtgcg gccccagctt gcggcctttg tccaattggt
    15361 ccacatgaaa ctggtagaaa taatcgccga tggctgctat gaactctttg tcgttcatcc
    15421 aggcgacatg cgtgctgccg cgaatactgg tatcgttttt ttcatacaac agcgtggtct
    15481 gcctgctcaa ttcgttcaac gaaaccggat cgtatagcat gaggtgctgc ccggtagtgc
    15541 cgaggtagcc gtaagtgccg cccgggctga tgtgcaacgc gtgaccgccg gccaggccat
    15601 tatagtaatt gattttgaaa gcaaacgagt tgtcggccgg gtcgaacaaa aaacagcaca
    15661 ccccggccat gccctcaatg ccgttgatgc cgttgccgtc taatgcgata tgaaaagcgt
    15721 atggatattt cttcatggtt tccgcgacag cgctcagctt gtttttcgag gcgttttggg
    15781 cttggcgccg attacagaag agctacgttt gtcgcttcca ggcatttttt caacgggcgg
    15841 agtatcagcc ttctccgcag gaggctgcac tgccacgatg aggtcgaata tagaatcatc
    15901 gtatccggcc accgtgacgg tattgcgata agctccggga ccggtcgttt caggaatatt
    15961 gacgctgaat cgcacctccg tggtttggcc gggaatcagc acaaacgact ctggctcaca
    16021 aaccagcaga agcggggccg ccgctccatt ggctgcatca acaaaacccg aaagtatgaa
    16081 cttgcaatgg cgcgtttcgg catcgcggct gttgaaatgc aacaaaaact caaggctctg
    16141 ccccgactcc ccggtcaggg tcattacctt gcgctccggg accggttcta tgtttgtctg
    16201 ctgtggagcc gctgccggag caggttgatg agccatgttc ttcgaccaat gcaactgcaa
    16261 atcatacaag ttctcagcat atcgcaactg aagtttcacc aaactcgtcc atgcttccga
    16321 gagggattcc atcacattac tgcccgacag ttccgtttta cctgagtaca ggttttgcaa
    16381 ggccgcagcg gcttctttgt aataagtcgt attggcttct atggcttttt tgactaccct
    16441 ttcaaagtct tttaccaaat ctccgtttgt gctttgctct gccattattc tgtgttttgg
    16501 gaatatgaaa atggatcagc cctgcgtacg gttggggctg cggtccggcg cttgttcgca
    16561 atagaagtga tcctgccagc tctgccagcg cataaaataa cgcttctcgt cgagcggacg
    16621 cacctccaca gcaggttggc aggccgtgac acacagttgg aagcagacgt tctgattcac
    16681 tttattttct ctgatgacga tttcggcctc gtaacaatcc ccggcccgaa acccctcgat
    16741 gaggctgagc gtgagggaga ccgtagcggt ttggcccggc tccaaatgca attcctgccg
    16801 gtccaaccag ggctgcaccg gcgcgggttc tccttgcgtg ttgaccattg gccgtatgcc
    16861 gatgcgatag gtgcgggcat tgccgcaggt gttttttaca ttgaatggaa ccaccatgac
    16921 ttcgccgggg caagcggagc gtttgatttc ggccaggcat cgcggcgggc aatcgggctg
    16981 cggcgggcaa acgccgccgg ggcgacagca gtcggaat
//
LOCUS       DAORLF010000040        16274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1918_length_16274_cov_2.244035, whole genome shotgun sequence.
ACCESSION   DAORLF010000040 DAORLF010000000
VERSION     DAORLF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_16274_cov_2.244035"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK198_05645"
     CDS             <1..215
                     /locus_tag="PK198_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF38246.1"
                     /translation="TIAWQWLRMAVAAQRALPDAAGEQEKAFYEGKILTAQYFIEYEL
                     PKTKGLHQRLSSPERVTMRTASEHLD"
     assembly_gap    255..328
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <329..1169
                     /locus_tag="PK198_05650"
     CDS             <329..1169
                     /locus_tag="PK198_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF38247.1"
                     /translation="FLVETSDGPALIETGPHSTFPSLAENLARYGYRPEDVRHVFISH
                     IHLDHAGAAWALAQLGAKIYLHPAGERHVVNPEKLLQSAKMIYKDQMDALWGDLQPID
                     PQNLVIAAHGDVFTVGEINFTAWHTPGHAVHHIAWQVGDSLFTGDVGGVKIHRGPVVP
                     PCPPPDINVEQWQESVALIRSLPVERLYLTHYGEITHIGEHLDVLEQTLLDWANWMRP
                     YFEAGESHEIITPLFAEYVHNQLKKYNLNAEEIEIYENANPAWMSVAGLMRYWKKREG
                     AFG"
     assembly_gap    1201..1215
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1321..1563)
                     /locus_tag="PK198_05655"
     CDS             complement(1321..1563)
                     /locus_tag="PK198_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38248.1"
                     /translation="MKKSNAQITLLAVLFLISLCSYAYLATRNSETIVPSGNDSSNTV
                     QVEEENKVKKSAKYFLLDVEIIKKLSEAARRLLPAS"
     gene            complement(1702..2376)
                     /locus_tag="PK198_05660"
     CDS             complement(1702..2376)
                     /locus_tag="PK198_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF38249.1"
                     /translation="MSNLIVRLNNANIYQQQQAVLRGVTMTISQGEFAYLIGKTGSGK
                     SSLLKVLYGALPLREGKGEVAGFDLLQLDRKKTPFLRRKLGVVFQDFNLLSDRNVDQN
                     LRFVLSATGWKNEQDIRNRIEEVLAKVSLSDKLHSMPHELSGGEQQRVVIARALLNQP
                     ELIIADEPTGNLDPDTSDEILQLLRRLAQVNNTAVLVATHDYRIIENFPARIIRCIQG
                     RIVEEV"
     gene            2505..3863
                     /locus_tag="PK198_05665"
     CDS             2505..3863
                     /locus_tag="PK198_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HRF38250.1"
                     /translation="MHPRRAIVWFRNDLRIHDNEALTEALRMADEIIPVYVFDERIFL
                     GHTRFGFRKTGPFRAKFIIESIKDLRNNLRALGSDLMVRIGQPEEILFHLANQTQSSW
                     VFCNRERTQEEVEVQDALEQKLWSTGREIWYCRGKMLFHTSDLPFPVTHTPDIFTQFR
                     KEVERFTPIREPLKSPGAGAFKPLTVHLESGSIPELEDVQHETFAHDARSALHFHGGE
                     TYALERLRYYLWDTNLVKCYKETRNELLGGDYSSKFSPWLAQGCLSPKQIYHELKRYE
                     AERGANDSTYWLFFELLWRDFFRLTAKKHGNRIFLRGGIKGEVNPQWRDDIKTLRKWI
                     NGQTGQPFIDANMRELAATGFMSNRGRQNVASYLVNDLKVNWQMGAEYFESLLLDYDP
                     ASNWGNWNYVAGVGNDSRENRYFNPKTQAERYDPNGVYVNYWLPDRATPAELVCETSK
                     VS"
     gene            3906..4769
                     /locus_tag="PK198_05670"
     CDS             3906..4769
                     /locus_tag="PK198_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRF38251.1"
                     /translation="MKSYFSLIKFSHTVFALPFAMLGFFLAVRHTDQGIPFRLFLLVL
                     LCMVFARSAAMAFNRYLDRDVDERNPRTRSREIPAGVISPSAALSFVVISSALFVACT
                     WFINPLCFALSPVALAVILGYSYTKRFTFLCHFVLGLGLSLAPIGAWLAVTGSFEVLP
                     LLYSAIVLLWVSGFDIIYALQDEEFDRSMQLRSVPVALGKAGALRLSSVLHVVCAALV
                     VVAAWQLEQHYPDFGWLHRGGALAFIGLLFYQHTLVKPNDLSRVNLAFFTTNGIAGVV
                     FGGLVILDLWV"
     gene            4821..>6132
                     /locus_tag="PK198_05675"
     CDS             4821..>6132
                     /locus_tag="PK198_05675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF38252.1"
                     /translation="MKLQFCGAAREVTGSSHLLTLDNGFKILLDCGLYQGSAKMMSDF
                     NPNWLFKPGEIDVLILSHAHIDHTGRVPYLVKSGFSGSIHCTHATRSLSALMLMDSAK
                     IQQRDAEFFNKRRKASQAERTPLYTPDDVKATMELFNSYNYGRWFKIHEDVEVLFRDA
                     GHILGSANVTLKIFENGKTTYLGFTGDIGRPDRPILRDPQPMPEVDYLICESTYGDKE
                     HLEGPEETERFLSIIQDTCVRRKGKILIPAFSLGRTQEIVYILDQLEHAGRLPHIPVF
                     VDSPLAVDVTAVFGAHPECYDENLHDYLQMDSNPFGFRSLQYIRGVEESKMLNALDEP
                     CIIIAAAGMLNAGRSRHHLFNTMDNPRNTILIVGYCSPDTPGGMLRNGVRELKLFGEV
                     KPLNAKVEIMDSFSAHADRREIVAFLSSQKRRQPHIFLVHGELDR"
     assembly_gap    6133..6145
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6235..7569)
                     /gene="purB"
                     /locus_tag="PK198_05680"
     CDS             complement(6235..7569)
                     /gene="purB"
                     /locus_tag="PK198_05680"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRF38253.1"
                     /translation="MLTAISPIDGRYHNRTESLSGYFSEYALMKYRVQVEVLYFKALC
                     EVPLPHLAGVQPEHLRILEQKMQDFSLQDAERIKAIERSTNHDVKAVEYYLKELMEEA
                     GMSQWKEFVHFGLTSQDINNTAIPLSVRDALHAEYLPVLEALTGTLRQLAADWAAIPM
                     LAHTHGQPASPTLLGKEMEVFAARLEAQIAQLRAVPMSAKFGGATGNLNAHFVAYPQI
                     NWHEFARQFVEKTLGLKRSYPTTQIEHYDNLAALFHAMMRVHTILIDLCRDMWTYISM
                     EYFRQRTVAGEVGSSAMPHKVNPIDFENAEGNLGMANAVLGFMAEKLPVSRLQRDLTD
                     STVLRNLGVPMGHALIAFHAAAKGLSKLQLNEAQLKTDLESNWMVTAEAIQTILRREC
                     YPEPYEALKALTRGKAAVQKADIHDFIDGLNVSEAVKSELKQIEPGNYTGRS"
     gene            7703..8506
                     /gene="proC"
                     /locus_tag="PK198_05685"
     CDS             7703..8506
                     /gene="proC"
                     /locus_tag="PK198_05685"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRF38254.1"
                     /translation="MKILIIGGGNMGMTYAQSFLRSHIAEKENMMILEKSPEKADELS
                     KKDIGAVYGKPEDCVPTADLIILAVKPQDSAALFGVIRHLVHPQQVFLSIMAGVKMES
                     IAKGLGVEKVIRAMPNLPAQIGMGMTAFSSSDEVTRIELVTVQNLLNTTGKTLYVANE
                     SLIDAATAISGSGPAYVWFFMRSLMEAAREMGFSMSEAELLVGQTFLGATELYNATNV
                     SCDTWIERVCSRGGTTEAALNTYLNNAVRADIIEGAMAALNRAEELGRN"
     gene            8545..>8830
                     /locus_tag="PK198_05690"
     CDS             8545..>8830
                     /locus_tag="PK198_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38255.1"
                     /translation="MGIKEKLTIEKLIEEAQLFCVEQSKIQHSELYGITDGKAVGTLI
                     EHKFQTHLNEKYKVLIGSSAKGIDLPSDDIETDIKVTSIKQPQSSCPFRDA"
     assembly_gap    8831..9040
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9041..9245
                     /locus_tag="PK198_05695"
     CDS             <9041..9245
                     /locus_tag="PK198_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38256.1"
                     /translation="AYLQDKNIPADEITLAKVAEQILQTPPEQGYLTISNALQWRLQY
                     QRIVTLANDIQGIEKIVNYNNPQ"
     gene            9242..>10328
                     /locus_tag="PK198_05700"
     CDS             9242..>10328
                     /locus_tag="PK198_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38257.1"
                     /translation="MMKVFEANITHQVSDYLNDNLKNVVSVEKANQKIYDAFGIMYFF
                     DNAEDLQILKEAISIPQNIVSEPDRTEYGDFQTNIELANKVAFHLTTKNILPEIIIEP
                     TCGKGNFITASLKHFSNVKKIFGIEIYKPYVWESKFNILDFYLSNPTEDKPEISIIHS
                     SIFDFDFKTIAKENGTKEILITGNPPWVTNSKLGSLNSSNLPKKTNFKNHNGLDAMTG
                     KGNFDIAEYITLMMIKNFQHQKGNIALLVKNTVIKNIVFDQFTKKYPIDNIEKHCIDS
                     KKEFNVSVEASLFYCRLNSKPAFHCAEFDFYNKSKARPSFGWLSDKFVSNIDTYSNAE
                     RIDGKCPFEWRQGIKHDCSAIMELDKMN"
     assembly_gap    10329..10367
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10368..11025
                     /locus_tag="PK198_05705"
     CDS             <10368..11025
                     /locus_tag="PK198_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38258.1"
                     /translation="VQLENDLVYGILKSSDLKNTVINEPRKYTIITQKKVGQETNYIK
                     YNYPKTYKYLSGHQDIFNSRKSSIYNNKPLFSIFGIGDYSFKPYKVAISGLYKTSHFT
                     LILPQHEKPVMLDDTCYMLGFDKLEFAVYSFILLNCKTTTQFLQSITFADAKRTFTKD
                     VLMRIDLLELAQSIDKSELQIELNYINKTYNFDLTLSCWRDFINEMTPIQMGQIELFA
                     "
     gene            11162..>12922
                     /locus_tag="PK198_05710"
     CDS             11162..>12922
                     /locus_tag="PK198_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF38259.1"
                     /translation="MKIIRELFLFFCLALLLSPAPAQEIPLIKNHLPETMQAANQNWS
                     VTQAPSRFIYAANTAGLARYDGARWQLLPLPNRQIVRAVAADDKGHVFCGGFGEFGFW
                     KADALGGMHYTSLSKGLRSERAGTEEIWHILITGGKVYFQSFSAMYCYDYQSVRELPL
                     PGNVMFMQKAGERVLLPVIGQGIFQKTKTDAFERLPGTEQLLDHIVVGMAMLPDGQLL
                     IGTDRQGLFLLKDGQLRPWNSQVQSAMTKDRLNKMLSLSDGSIAFGSVGGGLYIADAG
                     GRVVYHVHKERGLQNNTVLSLFQDQDGNLWAGLDKGLDLLALSAPLRFSIDRSGNLGA
                     VYTACEFQNTLYLGTNQGLFYKAPNDFWTPVAGIKGQVWELSAINGQLICGHNDGTFV
                     VQNGAARKISKVTGGWMTLLCPWDSTHWIQGTYTGLIVLKKNAGDGQLVFSHRIEGFG
                     EPIEYIAFDEDGSLWAAHPYKGLHRLKIGHDLAAVMEVRKMSASAGVNSEFNLSLTRL
                     SENVILHAADGFFSWNRTAQSWQPYALPGFRAGGGERAVVAAGGDAFFVVFSDRVVHV
                     DKGRQTVFNLRLVDDRPNIIS"
     assembly_gap    12923..12965
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12966..13952
                     /locus_tag="PK198_05715"
     CDS             <12966..13952
                     /locus_tag="PK198_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF38260.1"
                     /translation="CLEDGYAKWDARKAVTASRMEARPRITEVQIPGRKGPGDSLGIL
                     SGNTREITLQASENHLIIQFASFVFDRKPTYRWRLIGLNEDWSDWQENSSQDFGFLPP
                     GNYRFEVQSDHSEAIAGVDFRINPRWHQTWYMRSLFVLAAMAGGVVALVWYRRRIMRE
                     HRRLLLERERELHQQRIQERNNQLQEDILKKAKDLANSTMHLIKKNEVLLEIRQRLTE
                     LPRDARLGTQVQQLHRLVERELSSENDWEVFEENFNSVHEAFLRKMKHSFPELTPGDL
                     RLAAYLKMNLSTKEIAPLLGISIRGVENKRYRLRQKMNLPNDENLVEFLLEF"
     gene            complement(13949..>14835)
                     /locus_tag="PK198_05720"
     CDS             complement(13949..>14835)
                     /locus_tag="PK198_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRF38261.1"
                     /translation="RRHTYLTSLLGIRHLVVAVNKMDMIGYDEEAFYRIAAAFKQFSN
                     QLNFKTIHFIPVSALHGENIVQGSVHFSWYQGPALLQYLEEINTEDDHFADAARLPVQ
                     WVIRPQSDDLHDYRGYAGRLTSGTLRVGDAVGVYPGGQTSRIERIEMYGKDLAEAEAL
                     DSIVLHLQDDIDVSRGDIIAPLSAAPVIGQELKARICWMDDEKHLIPGGKYLLQHHGK
                     KVRCVVRDLVDKIQIDTLQFVDAQDVKLNDIATVGIKTASPLALDLYADNRSNGAFIL
                     IDEMTCRTVAAGMVLECS"
     assembly_gap    14836..14865
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14866..15208)
                     /locus_tag="PK198_05725"
     CDS             complement(<14866..15208)
                     /locus_tag="PK198_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF38262.1"
                     /translation="MNLLRFITAGSVDDGKSTLIGRLLYDSNAIFEDQLQAMRRASRF
                     SDTDTPDLAILTDGLKAEREQGITIDVAYKYFSTPRRKFIIADAPGHVQYTRNMVTGA
                     SNCDLAIILVDA"
     gene            complement(15224..16126)
                     /gene="cysD"
                     /locus_tag="PK198_05730"
     CDS             complement(15224..16126)
                     /gene="cysD"
                     /locus_tag="PK198_05730"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRF38263.1"
                     /translation="MNKAHTHLDWLEAEAIHILRETAGQFERPALMFSGGKDSITLVH
                     LALKAFRPGAVPFPLLHIDTGHNFVETLDFRDQLAEQTGMKLIVRYVEDSIRQGKVAD
                     PKGKNASRNALQSTTLLDAIEEFRFDACIGGARRDEEKARAKERVFSVRNEFGEWDPK
                     RQRPELWSIYNGKIHPGENVRVFPISNWTELDVWHYIQRENIALPSLYFAHEREVVRR
                     NGSWMAAEPCLNLDAQDKIVSKLIRFRTIGDITCTAAVESGASTVEDIVQELRDARFS
                     ERGARLDDLVSEAAMEDRKKAGYF"
BASE COUNT         3879 a         4044 c         4077 g         3850 t
ORIGIN      
        1 tcaccattgc ctggcaatgg ctgcgaatgg cggtggcggc tcagcgcgca ctgccggatg
       61 ctgcgggcga gcaggaaaag gctttttatg aaggcaaaat cctgacggca caatacttca
      121 tagagtatga actgccgaaa accaagggcc tgcaccagcg cctgagcagc cccgaacgcg
      181 tgacgatgcg cactgcttcc gagcatttgg attgatgacg aaactcgcag ctcatagctt
      241 ttttggaaca tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ttttagtgga aacctccgac ggtcctgccc
      361 tcatagaaac cgggccacat tccaccttcc cgtccctggc ggagaacctg gcgcgatacg
      421 gctaccggcc cgaagatgtg cgccatgtgt tcatctcgca catccacttg gatcatgcgg
      481 gggcggcctg ggctttggct cagttgggtg caaaaatata cctgcatcct gccggcgagc
      541 gtcatgtagt gaacccggag aaattgcttc aatcggccaa aatgatctat aaagatcaga
      601 tggacgccct ctggggtgat ttacaaccta ttgatcctca gaacttggta attgcagcgc
      661 atggcgatgt atttaccgtg ggagagataa attttacagc ctggcacacg cccggtcatg
      721 cggtgcatca cattgcctgg caggtgggcg acagcctgtt taccggtgat gtgggcggcg
      781 tcaagattca tcgcgggccg gtagtgccgc cctgtcctcc acccgacatc aacgtagaac
      841 agtggcagga gtccgtcgcg ctcattcgca gcttgccggt cgagcgcctg tacctcacgc
      901 actacggcga aatcacccac atcggcgagc atttggacgt gttggaacaa accctgctcg
      961 attgggccaa ctggatgcga ccgtattttg aagcgggcga aagccacgaa atcatcactc
     1021 cgctctttgc agaatacgtt cataatcaat tgaaaaagta caatctgaac gccgaggaga
     1081 tagaaattta tgaaaatgcc aatccggcct ggatgagcgt ggcggggctg atgaggtatt
     1141 ggaagaagcg ggaaggggcg ttcgggtagg aacgggtttt gttttgggtt tcaggtttcg
     1201 nnnnnnnnnn nnnnnggctg ccggctacaa gctgcaagcc gcgctgagta ccgcctaccc
     1261 cgcgccacgg cgcggccacc gggacacctc gcgcttagca ccgccaaccg cctaccgagt
     1321 tcaggaagcg ggaagcaggc gacgcgcggc ttcggaaagt tttttaatga tttccacgtc
     1381 gagaaggaag tatttggccg attttttcac cttattttct tcttcaacct gaacggtatt
     1441 ggaagaatca ttcccggaag gaacaatcgt ttcggagttg cgggtagcca gatacgcgta
     1501 gctgcaaaga ctgattagaa acaagactgc caacaaagtg atttgagcat tcgatttttt
     1561 catgacagat gttttgagca ggcggttttt cagcgaatca aatgtagcct gtaaacaatt
     1621 gaaatggccg ttttgctcgc tgaaattgca ttttctttcg atagacagca aaaaagacag
     1681 gccgggttgg aaaaggttgc atcaaacttc ttccacaatg cggccctgaa tgcaccgaat
     1741 gatgcgcgcc ggaaaatttt cgatgatgcg atagtcgtgt gttgccacca atacagccgt
     1801 gttgttgact tgtgccagac gacggagtaa ttgcagaatt tcgtcggagg tgtcaggatc
     1861 gaggttgccg gtaggttcgt cggcgatgat gagttcgggt tgattgagca aggctctggc
     1921 gatgaccacg cgctgttgtt cgccgcccga cagttcgtga ggcatactgt gcagcttgtc
     1981 ggagaggctc actttggcca atacttcttc gatgcggttg cggatatcct gctcgttttt
     2041 ccagccggtg gcgctgagta caaagcgcag gttttggtcc acgtttcggt cggagagcag
     2101 gttgaaatcc tgaaaaacaa cacctaattt ccggcgcaaa aaaggcgttt ttttgcggtc
     2161 caattgaagc aggtcaaagc cggccacctc acctttgcct tcgcggagcg gcagcgcgcc
     2221 gtagagtact ttgagcaggc tggatttgcc gcttcccgtt ttgccgatga ggtaggcgaa
     2281 ttcgccctga gaaatggtca tggtgacccc gcgcaacacg gcctgttgtt gttgataaat
     2341 attggcattg ttgagccgta caatgaggtt gctcatggcg cgttgttttg gaaaagcgta
     2401 caaatgtaat tcatagtttg ttaatgcagc gtgtattttc gcaaccaacc ctgcaagttg
     2461 ttgtttagct tgcgatgaac gattgttggc taaacaaacg cattatgcac ccacgccgcg
     2521 ctattgtatg gttcagaaac gacctgcgca tccatgataa tgaggccctt acggaagccc
     2581 tccgcatggc cgatgaaatc attccagttt atgtcttcga cgaaagaata tttctggggc
     2641 acacccgctt cggcttccgc aaaacagggc ctttcagagc caaattcatc atcgaaagca
     2701 tcaaagacct gcggaacaac ctgcgggcgc tcggatccga tctgatggta cgcatcgggc
     2761 aacctgaaga aattctcttt catctggcca atcagaccca atcgagctgg gtgttttgca
     2821 accgcgagcg cacgcaggaa gaagtggaag tgcaggatgc tttggaacaa aagctttggt
     2881 caaccgggcg tgaaatctgg tattgccggg gcaaaatgct ctttcacacc tccgatctcc
     2941 cgtttccggt aacgcatacg cccgatattt ttacccaatt ccgcaaagaa gtagaacgct
     3001 tcacgcccat tcgcgagccg ctgaaatcgc cgggagccgg cgccttcaaa cctctgaccg
     3061 tacatttgga gtcaggcagc atccccgaat tggaggacgt gcagcacgaa acctttgccc
     3121 acgacgcccg ctcggcgctt cattttcacg gcggcgagac gtatgcactg gagcggcttc
     3181 gctactacct ttgggatacc aacctcgtaa agtgttacaa agaaacccgc aacgaactgc
     3241 tgggcggcga ttattcttcc aaattctcgc cctggctggc acagggctgc ctctcgccca
     3301 aacagattta ccacgagctg aaacgctatg aggccgaacg cggcgccaac gattccacct
     3361 attggctatt ttttgaattg ttgtggcggg attttttccg gctcacggcc aaaaaacacg
     3421 gcaaccgcat ttttcttcgg ggcggcatca aaggcgaagt caatcctcaa tggcgcgacg
     3481 acataaaaac gctccgcaag tggatcaacg gccaaaccgg acagcctttc atagatgcca
     3541 acatgcgcga actggcggct acgggattca tgtccaaccg ggggcggcaa aacgtggcct
     3601 cttatttggt gaatgacttg aaagtcaact ggcaaatggg cgccgaatac ttcgaatcgc
     3661 tcctgctcga ctacgatccg gccagcaatt ggggcaactg gaactatgtg gccggcgtgg
     3721 gcaacgattc ccgcgaaaac aggtatttca atcctaaaac gcaggccgag cgctacgacc
     3781 cgaatggcgt gtatgtgaac tattggctac ccgaccgcgc aacgcccgcc gaactcgttt
     3841 gcgaaacctc aaaggtttcg taaacgtaaa aacctacctt tgcgccctcc aaatacaggc
     3901 caaccatgaa gagttatttc tcgctcatca agttcagtca cacggtcttt gcgctgccct
     3961 ttgccatgct gggcttcttt cttgccgtgc gccacaccga tcagggcatt ccgtttcgcc
     4021 tttttttgct ggtgttgttg tgtatggtct ttgcccgcag cgcggccatg gctttcaacc
     4081 gctatttgga ccgcgacgtg gacgagcgca atccccgcac ccgcagccgc gagattcccg
     4141 ccggtgtcat atcgccgagc gccgccctca gttttgtcgt catcagcagc gcgctgtttg
     4201 tggcctgcac ctggttcatc aatccccttt gctttgcact ttctccggta gcgctggccg
     4261 tcattctcgg ctacagctac accaagcgct tcacttttct ctgccatttt gtattgggtt
     4321 tgggactgtc tttggcgccc atcggcgcat ggctggccgt cacgggcagt ttcgaggtct
     4381 tgcccttgct gtattcagcc attgttttgc tttgggtttc cggcttcgac atcatctatg
     4441 ccttgcagga tgaggagttt gaccgttcca tgcaactccg ctccgtgccg gtggctctgg
     4501 gcaaagccgg agcgctccgt ctgtcgtctg tgttgcatgt tgtttgcgcc gcgctcgtcg
     4561 tcgttgcggc ctggcaattg gagcagcatt acccggattt cggatggctg caccggggcg
     4621 gcgcgctggc gttcatcggc ttgttgtttt accaacacac tttggtcaaa cccaatgatc
     4681 tcagccgggt caacttagcc ttttttacta cgaacggcat tgccggggtt gtttttgggg
     4741 gattggtaat tttagactta tgggtgtaac attatcccct tatttcctat cttgctcccc
     4801 aattaaccat aacatccgat atgaaactac aattctgcgg cgcagcgcgc gaggtcaccg
     4861 gttcttctca cctgctcaca ttggacaacg gcttcaaaat cctgttggat tgcggtctgt
     4921 atcagggttc tgccaaaatg atgagcgatt tcaaccccaa ctggctgttt aagcccggcg
     4981 aaatagacgt tcttatactt tctcatgccc acattgacca caccgggcgc gtgccttatt
     5041 tggtcaaatc cggcttttcc ggctccatcc attgcaccca tgccacccgc agcctcagcg
     5101 cgctgatgct catggacagt gccaaaattc aacaacgcga cgccgaattt tttaacaagc
     5161 gccgcaaagc aagtcaggcc gaacgcacgc cgctctacac ccccgacgac gtgaaagcta
     5221 ccatggaact gttcaacagc tacaactatg gccgctggtt caaaattcac gaggacgtag
     5281 aggtgctgtt ccgcgatgca ggccatattc tgggcagcgc caacgtgacg ctcaagattt
     5341 ttgaaaacgg caaaaccact tacctcggct tcaccggcga tattggccgc cccgaccgac
     5401 ccatcctgcg cgacccgcaa cccatgcccg aagtggacta cctgatctgc gaatccacct
     5461 acggcgacaa agaacacctc gaaggccccg aagaaaccga gcgcttcctc tccatcattc
     5521 aggatacctg cgtcaggcgc aaaggcaaaa tactcattcc cgctttcagc ctgggccgca
     5581 cacaggaaat cgtttacatc ctcgatcagt tggaacacgc aggccggctg ccgcacattc
     5641 cggtttttgt ggatagcccg ctggcggtgg acgtgacggc ggtgttcggc gcgcacccgg
     5701 aatgttatga tgaaaatctg cacgactacc tccaaatgga ttccaatccg ttcggattca
     5761 gaagtttgca atacattcgg ggcgttgagg aatccaagat gctcaatgcc ctcgacgagc
     5821 cgtgcatcat cattgccgcc gccggtatgt tgaatgcggg caggtcgcgg catcacttgt
     5881 tcaatacgat ggacaacccg cgcaacacca ttctcattgt gggctattgc tcgcccgata
     5941 cgcccggcgg catgttgcgc aacggcgtgc gggaactgaa actattcgga gaggtgaaac
     6001 cgctcaatgc caaagtagaa atcatggact ccttctccgc acatgccgac cgccgcgaaa
     6061 tagtagcgtt tttatcttcc caaaagcgcc gccaaccaca cattttcttg gttcacggcg
     6121 agctggatcg ccnnnnnnnn nnnnncagca gaagttcaaa gaatatctgg ctgagcacgg
     6181 cttcggggat gtggaaatac ccgaactggc gcaggagttt gatttgtaat acgctcaact
     6241 tctgccggta taattgcccg gttcaatctg cttcaattcc gatttcaccg cttccgacac
     6301 gttcagcccg tctataaagt catgtatatc agctttttgg acggctgcct tgcctcgcgt
     6361 gagggccttg agcgcttcgt agggttcggg gtagcactcg cggcgcaaga tggtttgtat
     6421 ggcttcggcg gtcaccatcc agttgctttc caagtcggtt ttaagttgag cctcattgag
     6481 ttgcaacttg ctcaggccct tggccgccgc atgaaaagca atgagcgcat ggcccatcgg
     6541 cacccccagg ttgcgcaaca cggtgctgtc ggtgaggtcg cgttgcagtc gggaaaccgg
     6601 caatttttcg gccataaaac ccagcacggc attggccatc cccaggttgc cttcggcatt
     6661 ttcaaaatca atgggattta ccttgtgcgg catggccgat gagcctacct cgccggccac
     6721 ggtgcgctgc cggaaatatt ccatcgaaat gtaggtccac atatcgcggc acaagtcaat
     6781 gagaatggtg tgaacgcgca tcatggcatg aaacagcgcc gccagattgt cgtagtgttc
     6841 gatctgagta gtgggatagg agcgtttcag gcccagcgtt ttttctacaa attgccgggc
     6901 gaattcgtgc cagttgatct gcggataagc tacaaaatgc gcattgaggt tgccggtggc
     6961 cccgccgaat ttggcgctca tgggcacggc ccggagttgt gcgatttggg cctccaaacg
     7021 tgctgcaaac acctccattt ctttgcccaa cagcgtggga gaagccggct gcccgtgcgt
     7081 gtgcgccaac atggggatgg cggcccagtc ggcggccaat tgccttaatg tgccggtaag
     7141 tgcctctaat acaggcagat attccgcatg cagcgcatcc ctgaccgaca ggggaatggc
     7201 cgtattgttg atgtcttgcg aggtgaggcc aaagtgaaca aactccttcc attgcgacat
     7261 gccggcctct tccatcagct ccttcaggta gtactctacg gccttcacgt cgtggttggt
     7321 gctgcgttct atggctttga tgcgctcggc gtcctgcaag ctgaagtcct gcattttttg
     7381 ctccaaaatg cgcaggtgtt cgggttgtac gccggccaag tgcggcagcg gaacttcaca
     7441 taaagccttg aaatacagta cctctacctg cacgcgatac ttcatcaggg catattccga
     7501 aaaataaccg gacagcgatt cggtgcggtt gtggtagcgg ccgtctatgg gcgaaatggc
     7561 agtgagcatg gcattggcgg cgcgcgggtt ttggggcgcg gcttacgcgg gcaaaggtag
     7621 tattgccggg ccgtgtacag aagaaattgg ccggacaagt gcaggctatt tgtttatttt
     7681 gccgtttaaa tcggattcaa ccatgaaaat tctcatcatc ggcggcggca atatgggcat
     7741 gacctacgcc cagagcttcc tgcgctcgca cattgctgaa aaggaaaaca tgatgatttt
     7801 ggagaagtcg cccgaaaaag cggacgagct ttccaaaaaa gacatcggcg ctgtttacgg
     7861 caagccggaa gattgtgtac cgacagccga tctcatcatt ttggcagtaa aaccacagga
     7921 ttcggccgcc ctgttcggcg tcatccggca tttggtacac ccccagcagg tgtttctctc
     7981 catcatggcg ggcgtcaaaa tggagtccat tgccaaagga ttgggcgtag aaaaagtcat
     8041 cagggccatg cccaacctgc cggcccagat aggcatgggt atgacggcct tcagctccag
     8101 cgatgaagtg acgcgcatcg aattggtgac ggtgcaaaac ctgctgaaca ccaccggcaa
     8161 aaccctctat gtggccaacg aatcgctcat agatgccgcc accgccatat ccggcagcgg
     8221 ccccgcgtat gtgtggttct ttatgcgctc gctgatggag gccgcccgcg agatgggctt
     8281 ctccatgtcg gaggcagaac tacttgtagg acagacgttt ctgggcgcca ccgagttgta
     8341 caacgccacc aatgtatctt gcgacacctg gattgaacgg gtttgctcgc ggggcggcac
     8401 caccgaagcg gccctgaata cctacctcaa caatgccgtg agggccgaca tcatagaggg
     8461 cgctatggca gcgttgaaca gggccgaaga actggggagg aactgattgg ggggtgttat
     8521 tactataaat tgtaacttgg tcgaatggga ataaaagaaa aactgacgat agagaagctg
     8581 atagaagaag cacaactttt ctgtgttgaa caatcaaaaa ttcaacatag cgaactctat
     8641 ggcataacag acggaaaagc cgttggaacg ctcattgaac acaaattcca aacacattta
     8701 aatgaaaaat acaaagtttt gataggttct tctgccaaag gaattgactt gccgtcagac
     8761 gacatagaaa ccgatataaa agtaacatct ataaaacaac cgcaatcgtc ttgtccattc
     8821 cgagatgcta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcttatttg caagacaaaa
     9061 atattcctgc ggacgaaatc acgttggcaa aagttgctga acagattttg cagacgccac
     9121 ccgaacaagg ctacttgaca atttccaatg ctttacaatg gcgattgcag tatcaacgaa
     9181 ttgttacttt agctaatgac attcaaggta ttgaaaaaat tgtaaattac aacaacccgc
     9241 aatgatgaaa gtatttgaag caaatattac tcatcaagtt tcggattatt taaatgataa
     9301 tctaaaaaac gttgtgtctg ttgaaaaggc taatcaaaaa atatatgacg ccttcggtat
     9361 tatgtatttt tttgataatg cagaagactt gcaaatactc aaagaagcta tttcaattcc
     9421 tcaaaatatt gtttcagaac ccgacagaac tgaatatggc gactttcaaa ccaatataga
     9481 attagcaaac aaagttgctt ttcatctgac aacaaaaaat atcttacctg aaattatcat
     9541 tgagccgact tgtggaaaag gcaattttat taccgcttct ttgaagcatt tcagcaacgt
     9601 aaaaaaaatt ttcggtattg aaatttacaa accttacgtt tgggaaagta aattcaatat
     9661 tcttgatttt tatttgagta acccaactga agacaaaccc gaaatttcga ttatccacag
     9721 tagtattttt gactttgatt tcaaaactat tgccaaagaa aacggcacaa aagaaatttt
     9781 aattaccggc aatccgcctt gggtcaccaa ctctaaattg ggtagtttaa attcgtctaa
     9841 tcttccgaaa aaaacgaact tcaaaaacca caacgggtta gatgcaatga caggaaaagg
     9901 caattttgat attgccgaat atattacttt gatgatgatt aaaaactttc aacatcaaaa
     9961 agggaatata gccttgttgg ttaaaaatac ggtaatcaaa aacattgttt ttgaccaatt
    10021 cacaaaaaaa tatcctattg ataacataga gaaacattgc attgacagca aaaaggaatt
    10081 taatgtttcg gttgaggctt cattgtttta ctgccgactc aattcaaagc ctgcattcca
    10141 ttgtgcagaa tttgattttt acaacaaatc aaaagcaagg ccgagttttg gttggttgtc
    10201 cgataaattt gtttcaaata tagacactta ttccaatgct gaacgaattg acggaaaatg
    10261 tccgtttgag tggcggcaag gcataaaaca cgattgttcg gccattatgg aattagacaa
    10321 aatgaatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt tcaattggaa
    10381 aatgatttgg tttatggcat tttaaaaagt tcagacttaa aaaatacagt tatcaacgag
    10441 ccaagaaaat ataccattat aacgcaaaag aaagtcgggc aagaaaccaa ttacatcaaa
    10501 tacaattacc ctaaaactta caaatattta agcggacatc aagacatttt taattcgaga
    10561 aagtcaagca tttacaacaa caaaccgcta ttttctattt tcggcatagg cgattattcg
    10621 ttcaagccat ataaagttgc catttcagga ctttacaaaa cgtctcattt tacacttatc
    10681 ctaccacaac acgaaaaacc tgtaatgctt gacgatactt gttatatgtt aggttttgat
    10741 aagttggaat ttgcagttta ttcgttcata cttttaaatt gtaaaacgac aacacagttc
    10801 ctgcaatcca tcacttttgc agacgcaaaa agaactttca cgaaagatgt tttaatgcgt
    10861 attgaccttt tggaattagc tcaatctatt gataaatcag agttacaaat agaactaaat
    10921 tatataaaca aaacatataa tttcgacttg acgttgagct gttggcgaga cttcataaac
    10981 gaaatgacac cgatacaaat gggacaaatt gaattatttg cataaacagg aaaagtactg
    11041 tgaataacaa gcgttttgtc caatggcagg tgacggattt ctattaaaca ttactgcctc
    11101 gccggcaggc aaactcgtgg ctcgcgcctc acagctttta gcaggcgact gcatagcgac
    11161 aatgaagata atcagagaac tatttttgtt tttttgctta gcgctgctgc tttcaccggc
    11221 cccggcgcag gaaattcctt tgattaaaaa ccacctgccg gaaaccatgc aggccgccaa
    11281 tcaaaactgg tcggtcacac aggcgccgtc ccgctttatt tatgcagcca acaccgccgg
    11341 attggcccgg tacgacggcg cgcgctggca gttgcttcca ttgcctaacc gacagattgt
    11401 cagggcggta gctgccgacg acaaaggcca tgtgttttgc ggcggctttg gtgagtttgg
    11461 tttctggaaa gccgacgctt tgggcggaat gcactacacc tcgctgtcga aggggctgcg
    11521 gtcggagcgg gcaggcaccg aggaaatctg gcacatcctc atcaccggcg gcaaggtgta
    11581 tttccagtcg ttttcggcca tgtattgcta tgattatcaa tcggttcggg agcttccact
    11641 gccgggcaac gtcatgttta tgcaaaaggc tggcgaacgg gtattgctgc ctgtgatcgg
    11701 gcagggaatt tttcaaaaaa ccaaaaccga cgcattcgaa cgcctgccgg gaaccgagca
    11761 attgctggat catatcgtcg tgggtatggc catgctcccc gacggccaac tgctcatcgg
    11821 caccgatcgg caagggcttt ttttattaaa agacggccaa ctccgcccct ggaactcgca
    11881 ggtgcaaagc gccatgacca aagaccggct gaacaaaatg ctctccctct ccgacggcag
    11941 catcgctttc ggttcggtcg gcggcggcct gtacattgcc gatgccggcg ggcgcgtggt
    12001 ttatcatgta cacaaagagc ggggcttgca gaataacacg gtgctgtctt tgtttcaaga
    12061 tcaggacggc aacctctggg ccggtttgga caaaggtttg gacctgctgg cgctgagtgc
    12121 tccgctgcgg ttttccatag accggagcgg caatctgggc gcggtttaca cggcctgcga
    12181 atttcaaaac acgctctatc tgggcaccaa tcagggtttg ttttacaaag cgcccaatga
    12241 cttctggact ccggtagccg gcatcaaagg gcaggtttgg gagctatcgg ccatcaatgg
    12301 ccaactgata tgcggtcaca acgacggtac ttttgtggtg caaaacggcg ccgcacgcaa
    12361 gatttcaaaa gtgaccggcg gctggatgac cctgctttgt ccctgggatt caacacactg
    12421 gatacaaggc acttatacag gtttgattgt tttgaaaaaa aatgccggcg atggccaact
    12481 cgtcttttcg caccgcatag agggctttgg agaacccatc gaatacatcg ctttcgacga
    12541 agacggcagc ctctgggcgg cgcacccata caaagggttg catcgcctca agataggcca
    12601 cgatctggcc gccgtgatgg aagtgcgcaa aatgagcgcg tcggcgggag tcaattctga
    12661 gttcaattta tctttgaccc ggctctccga aaacgtgata ctgcacgctg ccgacggctt
    12721 tttttcgtgg aaccgaacag cccaaagctg gcagccttat gccttgcccg gctttcgtgc
    12781 gggcggcggc gagagggccg ttgttgccgc cggaggagat gcgttttttg tggtgttctc
    12841 cgaccgcgtg gtacacgttg acaaggggcg gcaaacggtt tttaacctgc gtttggtgga
    12901 cgaccggccc aacatcattt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnntgcct cgaagacggc tacgccaaat gggatgcccg taaagccgta acagcctcgc
    13021 gaatggaagc gcggccaaga ataacggaag tgcagattcc cggtcgcaaa ggcccaggag
    13081 acagcctcgg cattttgtcc ggcaacacaa gagaaatcac cctacaagct tctgaaaacc
    13141 atttgattat tcaattcgct tcttttgttt ttgatcgaaa acccacatac aggtggcggc
    13201 tcataggatt gaatgaagac tggtcggact ggcaggaaaa cagcagccag gattttggtt
    13261 ttttgccgcc gggcaattac cgtttcgagg tgcaatccga tcattctgag gcgattgcag
    13321 gcgttgattt tcgcatcaat ccgcgctggc atcaaacctg gtacatgcgc tccctgttcg
    13381 ttttggcggc tatggccggc ggcgtagttg cgctggtatg gtatcgccgc agaatcatgc
    13441 gtgaacaccg acgcctcttg ctggagcggg aacgggaact gcatcagcag cgcatacagg
    13501 agcgcaacaa tcaattgcag gaagatattc tcaagaaagc caaagactta gccaattcta
    13561 ccatgcacct cattaagaaa aatgaagtct tgttggagat cagacaacgc ctcacagagc
    13621 tgccccgcga cgcccgactg ggaacgcagg tgcagcaatt gcatcgcttg gtagagcggg
    13681 aactgagcag cgagaacgac tgggaggtgt ttgaagagaa tttcaactcg gtgcatgagg
    13741 cctttttgcg caaaatgaaa cacagcttcc cggaactcac gcccggagat ttgcggctgg
    13801 ccgcctacct caaaatgaac ctgagtacca aagaaatagc gcccctgctg ggcatttcca
    13861 tccggggcgt agagaacaaa cgctaccgcc tgcggcaaaa aatgaacctg cccaacgatg
    13921 aaaacttagt ggagtttttg ttggaatttt aagaacactc cagcaccatc cccgccgcca
    13981 ccgtccggca agtcatttca tcaatgagga taaaagcgcc gttgctgcgg ttgtcggcat
    14041 acaaatccag cgccagcggc gaggctgttt tgatacccac cgtagcgatg tcgttgagtt
    14101 ttacatcttg tgcatcaacg aattgcagcg tgtctatctg aattttatca accaagtccc
    14161 gcaccacgca gcgcactttt ttaccatgat gttgcagcag gtatttgccg ccgggaatca
    14221 gatgcttttc gtcatccatc cagcaaatgc gcgctttcag ttcttgaccg atcaccgggg
    14281 cggcgctcag gggcgcaatg atgtcgcctc tactcacgtc aatatcgtct tgcagatgca
    14341 gcacaatgga gtcaagcgcc tctgcttccg ccaggtcttt gccgtacatt tcaatgcgct
    14401 caatgcggct ggtttggccg ccgggataaa cgcccaccgc atcgcccacg cgcagtgtgc
    14461 cgctcgtcag gcggcctgca taccctctgt aatcgtgcag atcgtcgctt tgcgggcgaa
    14521 tgacccactg aacaggcagg cgggcggcat cggcaaagtg atcgtcttcc gtattgattt
    14581 cttccagata ttgcagcagc gcaggcccct gataccatga aaaatgaacg ctgccctgca
    14641 caatgttttc gccgtgtaag gcactgaccg gaatgaagtg tatggttttg aaattcagtt
    14701 gattggaaaa ctgtttgaac gctgcggcga tgcgataaaa cgcctcctca tcatagccga
    14761 tcatgtccat tttgttgacg gccaccacca aatgccggat gcccagcagc gaagtgaggt
    14821 aggtgtgccg gcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggca tccacgagaa
    14881 taatggccag atcgcagttg gaggctccgg tcaccatgtt gcgggtgtat tgcacatggc
    14941 cgggcgcatc ggcgatgatg aacttgcggc ggggcgttga aaaatatttg tacgccacat
    15001 ctatggtgat gccttgttcg cgctcggctt tgaggccgtc ggtgaggatg gcgaggtcgg
    15061 gagtatctgt gtctgaaaac cgggaagcac ggcgcatggc ctgaagctgg tcttcaaaaa
    15121 tggcgttgga atcgtacaaa aggcggccaa tgagggtgct ttttccgtcg tccacgctgc
    15181 cggcagtgat gaatcgtagt aagttcataa tgttttgttt gtgctagaaa taccctgctt
    15241 ttttgcggtc ttccatggcg gcttccgaaa cgagatcgtc taagcgggcg ccgcgttcgc
    15301 tgaatctggc gtcgcgcaat tcctgaacga tgtcttcaac agtggaagcg ccggactcta
    15361 cggcggcggt gcaggtaata tcgccgatgg tgcggaatcg aatgagcttg gagacgattt
    15421 tatcctgagc gtccaagttc aggcaaggtt cggcggccat ccaggagccg tttcggcgca
    15481 ccacttcccg ctcatgtgca aagtagagcg aaggcagggc aatgttttcg cgctgaatgt
    15541 agtgccaaac gtccaattcg gtccagttgc tgatgggaaa cacccgcaca ttttcacccg
    15601 gatggatttt gccgttgtag atgctccaaa gttcgggtct ttggcgttta gggtcccatt
    15661 cgccgaactc attgcgcacc gaaaaaacgc gctctttggc ccttgctttt tcctcatccc
    15721 ggcgcgcgcc accaatgcag gcatcaaacc ggaactcctc aatggcatcc agcaaggtag
    15781 tgctttggag ggcgtttcgg ctggcgtttt tgcctttggg gtctgccacc ttgccttgcc
    15841 gaatgctgtc ttccacatac ctgacgatca gtttcatgcc ggtttgttcg gccaattggt
    15901 ctctgaaatc gagcgtttcc acaaagttgt ggcccgtatc aatatggagt aaggggaacg
    15961 gaaccgctcc gggccggaat gccttcagcg ccagatgaac caatgtgatg gagtctttgc
    16021 cgcccgaaaa catgagcgcc ggtcgttcaa attgcccggc cgtttcgcgc aggatgtgaa
    16081 tggcttctgc ctccaaccaa tcgaggtgcg tgtgtgcttt attcatgcct ttagatcttt
    16141 agttactatt tagccggtcc cccaaagcta cgaccacgcc ggggcgtggc acgagctatg
    16201 agcttgtttt ctcgttcaga aattagattt tccatttggg gaaatttcaa aatctcatct
    16261 tgaaaaaagg ccct
//
LOCUS       DAORLF010000041        15959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1971_length_15959_cov_2.190707, whole genome shotgun sequence.
ACCESSION   DAORLF010000041 DAORLF010000000
VERSION     DAORLF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_15959_cov_2.190707"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PK198_05735"
     CDS             complement(<1..1524)
                     /locus_tag="PK198_05735"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRF38264.1"
                     /translation="MKMLFRFFPILLLAAVAIIVPSCVDLNFDEPPLEGEDPGLTANT
                     TIAQLKSRLVTPGSFVAIEDDVIIKGVVVADDRSGNFFNSFILQDETAGIDVRISISN
                     AYNFFPIGREIYIKCKGLVLGDNSGVTQLGGYTYTQTGSVRLGNIVNTGAFIFRGKKA
                     GELAPKVKTINGLVGGDITTLVKLENVEFVPADTAELFADAVGLRTLNRTIQDCNGNT
                     IVVRTSGFATFAVDLTPKGKGSITGIYTVFGTTKQLLIRELSDVQMNDTRCGGGGGPV
                     GDIITIASLRSLFAGGATSGPTGKSIEGVVISDRATASVDTRNLVLQDETGGIVVRFT
                     AAHNFALGEKVEVSVGGVELSEFRGLLQVNNTPAGAAVSKGPGTLPTPRTATVAQLNA
                     NAEAWESTLVKIDNATLSGGTGGTYSGNVTTNDGTGSTVIFTRSASTFAGQTYPSGTV
                     SVTAIVSQFDTPQLIIRAPSDVTGGGSGGNPDLLSVAQVRALFSGTTTNVAANKQIRG
                     "
     gene            complement(1618..>3673)
                     /locus_tag="PK198_05740"
     CDS             complement(1618..>3673)
                     /locus_tag="PK198_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF38265.1"
                     /translation="GTFRNRIMGTWSTGLMQNGWAFTLSGSRRWAQEGYVEGTFFDGW
                     AYFLSVDKKIDKDHSLNFTAFDAPIRRGRSTSSVSELYDLTGTNFYNPLWGFQNGEKR
                     NSSEVRTQRPVFMLRHDWNIHKNAVLITAASMQIGKDGNTGLDWFDAPDPRPSYYRYL
                     PSYVNQQSTEEGGQVAEDFRNNPNISQINWAALYDVNRNSLLNQKYANLLNGQTPEGA
                     WSQYVIEERRSDIRDFSVSSNYQATLTDNFKLHAGAVYQRQTVQNFKVIDDLLGGDYA
                     VNVNRFVIRDFPGNFDVAQNDLNDPSRIIHEGDRFGYDYDANIRRGELWAQGVYVLPN
                     VEAFISGSIGQTTFWRNGKMRNGMFPDNSFGNSEKQNFLNVGGKAGVTYKLDGRNYFF
                     VNGFYQTRAPFFDNSYISPRTRADLIPNLTNEKILSVEGGYLLRAPNVKARAVAYLTQ
                     FSDQARILRFYNDDLEGAFVNYVITGQDPRHAGLELALEAKISPSLKLSGVAALGQYI
                     YNSRPTGNVYLDNPRDLTLGDNRAFDIYAENFRLAGMPQTAYTAGINYSSPKFWFVNL
                     NFNYFDNIWVDFNPIRRTANAVYGLDPTDPLLRNIVDQEQAPATFTMDFFGGKSWRIR
                     SKYYLYLNVGVNNVLDNKNFITGGFEQLRFDFETRNPDLFPNRYFYNFGRNFFINLSL
                     RI"
     assembly_gap    3674..3683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3684..4416)
                     /locus_tag="PK198_05745"
     CDS             complement(<3684..4416)
                     /locus_tag="PK198_05745"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF38266.1"
                     /translation="MLRHLAWAFVLNAFFISFLSTASAQNARITGVVLDQSNQQPAQG
                     AVVALQGTSFSTLSDEQGRFSLQNIPAGAYKLNIRLDNFVEYNLDVSIAGKDLNLSTI
                     GLAPRGEAGVALKEDLIPTISLSDDNLEVEGNNQNISGILTASRDVFVSAAAFTFGPA
                     RFRVRGYDSEYVNVFLNGLPFNDLDNGRVTWNVWGGLNDVTRNREVDLGTGPISYTFG
                     GVGGGNAIDTRASFHRKQLRLSYSLS"
     gene            4553..5599
                     /locus_tag="PK198_05750"
     CDS             4553..5599
                     /locus_tag="PK198_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF38267.1"
                     /translation="MKNHLFVLAFILLPLAAFSQPKQYKIAAIGFYNLENLFDTINNP
                     LTNDEDFLPTGSYSYTTAVYVEKQANMARVIADMGTELSPDGVAILGVAEVENRKVLE
                     DLVAQPTIKDRKYQIVHYDSPDPRGIDVGLLYQAKYYTVTGSKAIPMVAYEEGGEARG
                     TRDILLVSGIMDGDPLHVIVGHWPSRRGGEAATRPLRNAAALICKNVADSITLADPNA
                     RVIIMGDLNDDPVSPSVRQVLNAKAKKEDTPDRGFFNPFYDFYKKGIGTLAYQDSWNL
                     FDQVILSQSLVNPKLGGYQYYQARVFNAPYLYQQTGHFKGYPHRTFSGSTYIGGYSDH
                     FPTFVYLVKEISGK"
     gene            complement(5930..6934)
                     /gene="add"
                     /locus_tag="PK198_05755"
     CDS             complement(5930..6934)
                     /gene="add"
                     /locus_tag="PK198_05755"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRF38268.1"
                     /translation="MTSEFLHLPKIELHLHLDCSLSFDVVRKIKPDTDWETYRRDYVA
                     PSKCTDLADYISRAVSAISLMQTREHLRWVTLDLMEQLREDGAIYAEVRFAPLLHTQQ
                     GLAPAEVVQTVEAALAEGMAQTGVEARLILCTLRNFSEAQSMETVRLTEQFAGTLVVG
                     FDIAADEAGFPIDNHISAFAYAREKGIHVTAHAGEAKGPESVWETLQHFHPARIGHGV
                     RSAEDEALLAFLKSEDIHLELCPTSNVQTNVVATIEDHPAEKIWRNGVSMSINTDGRA
                     LSDVTLTQEYDTMRRVFGWQKERLLQCNLEALRHAFIPEEVKARLRARLLEGWGGFQE
                     "
     gene            complement(7024..8700)
                     /locus_tag="PK198_05760"
     CDS             complement(7024..8700)
                     /locus_tag="PK198_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF38269.1"
                     /translation="MADEKPWLSLYPKGVPANIDADSYPTLTAMLDETFKKFKNRPAF
                     SCMGKTLSFAEVDQLSTQFGAYLHSRGLQPGDRIALMMPNLAQYPIALFGALRAGLVI
                     VNTNPLYTPREMLHQFNDSGAKAIIIAENFASNLEKILSQTQIKTIILTSIGEMLGFP
                     KGAIVNFVVRSIKKMVPKFTLPNTVSFTQAMEGGRKFSLPQFQGNSDDVILLQYTGGT
                     TGVSKGAMLTNRNLVANMVQMKAWMQPFLKEGEEVALCPLPLYHIFAFSVNCLALMSQ
                     GAENVLVTNARDIPSLIKIMKARPVSLVSGVNTLFNALLNHPDFASCDFSRLKITVGG
                     GMAVQKPVAERWQQVTGCFLSEGFGMTEASPVITANPFDGSGRLGTIGLPFPSTDVRI
                     IDETGKVLSVGEIGEIQCRGPQVMKGYYNRPDATADTIREGWLCTGDIGVMEPDGYFR
                     IVDRKKDMILVSGFNVYPNEIEEVVVSHPKVLEAAAIGLPDEKSGEVVKIFVVKKDAS
                     LTESELLAFCRENLTGYKMPKSVEFRTELPKTNVGKILRRELRDEAVKKA"
     gene            8938..9375
                     /locus_tag="PK198_05765"
     CDS             8938..9375
                     /locus_tag="PK198_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HRF38270.1"
                     /translation="MLYQILDHAHSGFRWVVLLLLLAAIFSAFQAWQSGRAGASKMSF
                     YALMAAHIQLLGGLILYFISPYVKFEGGVMKDAVARFYTVEHISMMLIAIALITVGYS
                     KAKRAANDAARGKTTFWFYLIGLLVILAAIPWPFRSGLGGNWF"
     gene            complement(9558..>11153)
                     /locus_tag="PK198_05770"
     CDS             complement(9558..>11153)
                     /locus_tag="PK198_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38271.1"
                     /translation="GSTLPNGEYRINVPPGSYTVSVVLPSSNVWNIQCNNAQPVVFAE
                     SMEQALGVNFGVRSDIDCPQPEVNIGIPFLRRCVENTYTVRYTNQGSELIEDAYVNVT
                     LDPFFTFVGSSLPVASVNGSTYRFDVGDLDVFETRTFTVTISVSCEAELGQTHCISAE
                     MFPVAPCMIDPNWSGASLRVEGECQGDRVVFTITNEGEDMNDPVEYIVIEDIMIQIME
                     DDLLLGSGQMRQVELPANGSTWRLELRQVAFHPDNNYQAAFVEGCGTGESSFGFINQF
                     ALGDEKPNYDLDCRDNIGAYDPNDKQGFPLGVGEQGFIRPGQPLDYLIRFQNTGTDTA
                     FTVIIRDTLSEWLDPASIRPGTSSHPYAFNFSDENAIQFVFSNIMLPDSNVNEPASHG
                     FVRFSIRQRPDVPLGTAIRNSAGIYFDFNEPVITNTTLHTVQENFLITVDVKDVALRP
                     GLELTVFPNPAAHSAQFRLSSGGIERGLLQVWNAQGQLVGTQPFSGDQTTWNASGMRQ
                     GLYFFQIVSGGQTVASGKLLVRR"
     assembly_gap    11154..11227
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11228..11910)
                     /locus_tag="PK198_05775"
     CDS             complement(<11228..11910)
                     /locus_tag="PK198_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRF38272.1"
                     /translation="MSAIYTMFRLIAGLMLMAVSVSLSAQQATISGRIATRNNESVSG
                     VTIFVTGQVNAQTLTDENGNYAVQVPVGGSYVVRPEYDLDPLNGVSTLDAVLIVRHIE
                     GTEPFTSPYQFIAADVDRNSIINALDTFDLRNLILGVTEEFPNNSSWRFVDAAYQFPN
                     PANPLSLAYPGSVVVSKFSADVTGVNFVAVKTGDVNSTSLTSLRIQEVHFSSIRGRVF
                     HDANDNCQG"
     gene            12093..>13367
                     /locus_tag="PK198_05780"
     CDS             12093..>13367
                     /locus_tag="PK198_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38273.1"
                     /translation="MENTRLWQLLLTLTPEERQGLKKFVESPFFNQRPEAARLLDALL
                     ISFKKGRPAPGKEQVFEQVFEGRDFDDHRLRMAISFLYQLARQFLTVQDFLEDEVHVA
                     IRESEVLRRRKLYALSRHCLDSARNTLDVHKVRNADFFDQEYRLLLEECRLEMSRPGA
                     QPERLQALSEQLDKAFLSRKLWQNCALLSHQNMAKSDFGPGLLDHALDGLDEPAVPAI
                     AIYFWYYRALTQPENSEQHYQRFKKMLFEHADIFPGEELRDLYLLAINFCIKKYNAGN
                     QAFLREQLELYQRGLEHRYFLVAGELSAYTYLNVVTLALVLQELDWAAQFIEAYRPML
                     APERQESFFSFNKARLEYAQKRPGHALQLLQKAEYKEVLLGLAAKTLQLKIYYESGEF
                     DLLDAHLQSLKTYLYRKKVMGYHRENYMNTIRF"
     assembly_gap    13368..13493
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13517..14491)
                     /locus_tag="PK198_05785"
     CDS             complement(13517..14491)
                     /locus_tag="PK198_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF38274.1"
                     /translation="MKNYKILKMCFLCTALCVGATELAAQQTPIFTVYRDQWSLLNPA
                     ALSGHYVLNNRTMTLSATWREQWWNVPESPRTQWLNWEWVQEKFNSVWGLALLNDRTG
                     KIGQTGVYGRYAYRMEMGRRTTHTLTIGLHAGLVQYRAKVSEIEFPNPADVALSNVTT
                     LRPDIGMGAFYQHSDKYYVGISVPQTFGFFTPFRQENFEIGIRRVPHAYLVLGGYLDT
                     PWLGNTTSFAEPSLWLKYAPGGGLNADANVRLQVSELVWAGTGLNVSMGQAPGMTLHA
                     EAGVLLGEQVRIADGQLKIGFGFDVGLTHGAGRSLGNSAELNVSYAWK"
     gene            complement(14506..>15170)
                     /locus_tag="PK198_05790"
     CDS             complement(14506..>15170)
                     /locus_tag="PK198_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF38275.1"
                     /translation="QRGPNRFVWQVSLEGCAFSAADTVTVTVEAPPVLNDDRYTLQRV
                     QDVLVMEVLLNDNLSGLPDTVLSYLGGPAVGQLELLDGRRFRYTVTEDFRGRVSFQYV
                     VCNPGARCGTACDTATVTIEVLNMPAVPGGLIVDDPGLNGQLTIKGLNGFSRVEMQIF
                     SRWGDLVFEEKQYDNGNPWRGHAHRSGNALPEGAYYYVLRAFDGNVQVGEAQRGVVHL
                     FR"
     assembly_gap    15171..15180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15181..>15959)
                     /locus_tag="PK198_05795"
     CDS             complement(<15181..>15959)
                     /locus_tag="PK198_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38276.1"
                     /translation="LDGQQNPTGLIAGTYSVTVTDAGGCTATRSINLADPPALQLNVS
                     ATPETTAGVNDGTATAAATGGTGGYTFAWNNGGNTPTISGLAPGNYSVTVTDGNQCTI
                     SGSASVAAGQGGGGACVAQPVYAVAAPARVCQGDTIRLEADDLYPSPNVRYVWFIPNG
                     DSLTTVLPRVGILAASGAYGGQYFALRDSSGCRSIRVGGAPVQVQSLPDDAATVAMLD
                     TVLCAAGTVTLRALPALGGTGRWLPLNAATVDNAASEQTLAR"
BASE COUNT         3568 a         4441 c         4153 g         3577 t
ORIGIN      
        1 gccgcgaatc tgcttgttgg ccgccacatt ggtagtcgtg ccgctgaaca gtgcgcgcac
       61 ttgtgccacg ctgagcaggt cgggattgcc gccgctgccg ccgccggtca cgtcgcttgg
      121 ggcgcggatg atgagttgag gggtatcgaa ctgcgaaaca atggccgtca cgctgaccgt
      181 accggaagga tacgtttggc cggcaaaagt agaagccgag cgcgtgaaga tgaccgtaga
      241 gccggtaccg tcgttggtcg ttacgttgcc ggaataagtg ccgccggtgc cgccgctgag
      301 ggtggcattg tcaattttca ccaaagtgga ctcccaagcc tcggcattgg cattcagttg
      361 tgccacagta gccgtgcggg gtgtaggcaa tgtgccgggg cctttcgata cggccgctcc
      421 tgcaggcgtg ttgttcactt gcaacaggcc acggaattcc gacaattcta ccccaccgac
      481 agagacctcc actttttcgc ccaacgcgaa gttgtgggct gccgtaaagc gcaccacgat
      541 gccgccggtt tcgtcttgca gcacgaggtt gcgggtgtcc acgctggcgg tagcgcggtc
      601 agaaataaca actccttcga tggatttgcc ggtcgggccg gaagtggcgc ctcctgcaaa
      661 caagctccgc aacgatgcaa tggtgatgat gtcgcccacc gggccgccac cgccaccgca
      721 acgggtatcg ttcatttgca catcgctcag ctctctgatc aacaactgct tggttgttcc
      781 gaaaacggta tatatgccgg tgatggagcc ttttcctttg ggagtgagat caacggcaaa
      841 agtggcaaaa ccactggtgc gcaccacgat ggtattgccg ttgcaatcct ggatggtgcg
      901 attgagcgta cgaagtccca ccgcatcggc gaagagttcg gcagtgtcgg cgggcacaaa
      961 ttccacgttt tccaatttga ccaaagtggt gatgtcgccg cctaccaatc cattgatagt
     1021 cttcacctta ggagctaatt cgcctgcttt tttacctcta aaaatgaaag cgcccgtatt
     1081 aacgatattg cccaaacgca ccgaaccggt ttgggtgtag gtgtagcccc ccaactgcgt
     1141 cactccgctg ttgtcgccga gtacaagccc cttgcacttg atgtaaattt ccctgccgat
     1201 ggggaagaaa ttgtaagcat tggaaatgct gatgcgcacg tctatgccgg cagtttcgtc
     1261 ctgcaggatg aaagagttga aaaaattgcc ggagcggtcg tcggccacca ccacgccttt
     1321 gatgatgacg tcatcttcaa tagcgacgaa ggaacctggc gttactaggc ggcttttgag
     1381 ttgcgcaata gtggtattgg cagtcagtcc ggggtcttcg ccttccaatg gcggttcgtc
     1441 aaaattaagg tccacacagg aaggaacgat gatagccaca gcggccagaa gaaggattgg
     1501 gaaaaagcga aaaagcattt tcattggttg tattttttat tgctaaagta catgttgacg
     1561 ggctgatgct tacggctccc tactcgtagc tcgtagctcg cggctcgtag cttgaagcta
     1621 tatcctcaag ctcaggttaa taaagaaatt ccggccaaaa ttgtagaagt agcgattggg
     1681 aaacaggtcc ggattgcggg tttcaaaatc gaagcgaagc tgctcgaaac cgccggtgat
     1741 gaagttcttg ttatccagca cattgttcac ccctacgttc aaatacagat agtatttact
     1801 gcggatgcgc caggatttgc ccccgaaaaa gtccatagta aaggtggcag gcgcctgctc
     1861 ctggtccacg atattgcgca acagcgggtc ggtaggatca agaccgtaca ccgcgttggc
     1921 tgtgcggcga atggggttga aatccaccca gatattatca aagtaattga aattgaggtt
     1981 cacaaaccag aattttggcg agctgtaatt gatgcccgcc gtataagcgg tttgcggcat
     2041 accggcgagg cggaagtttt cggcgtagat gtcaaatgcg cggttgtcgc ccagtgtcag
     2101 gtcgcgcggg ttgtccaaat agacattgcc ggtgggccgg gaattgtaaa tgtactgccc
     2161 cagtgccgcc acgcccgata gcttgaggct gggcgaaatt ttggcttcca gtgcgagttc
     2221 taagcctgca tggcggggat cctgcccggt gatgacgtag ttgacgaatg cgccttccag
     2281 atcgtcgttg taaaaacgca ggatacgcgc ctgatcggaa aactgcgtga ggtaagctac
     2341 cgcgcgagcc ttcacattgg gtgcgcgcag gaggtacccg ccttctacgg agaggatttt
     2401 ttcgttggta aggttcggaa tcaggtcggc acgagtgcgc ggcgagatgt aggaattatc
     2461 gaaaaacggt gcgcgggttt gataaaaacc attgacaaag aagtagttac gaccatccaa
     2521 cttgtatgtc acgccggctt tgccgcccac attgaggaag ttttgctttt cggaattgcc
     2581 gaacgaattg tctggaaaca ttccgttgcg catcttgccg ttgcgccaga aggtggtctg
     2641 gccgatgctg ccggagatga aagcctccac attgggcagc acataaacgc cttgcgccca
     2701 gagttcgccc cgccggatat tggcatcgta atcgtagccg aaacggtcgc cctcgtgaat
     2761 gatgcgggaa ggatcgttga ggtcgttttg agccacgtcg aaattgccgg ggaagtcgcg
     2821 aatgacgaat cggttgacat tcacggcata atcgccgccg agcaggtcgt ctatcacctt
     2881 gaaattctgc acagtctggc gttggtaaac ggccccggcg tgcagtttga agttgtcggt
     2941 cagcgtagcc tgatagttgg agctgacgct gaaatcgcgt atgtcgctgc gacgttcctc
     3001 gatgacgtat tgcgaccaag caccctccgg cgtctgacca ttgagcagat tggcatattt
     3061 ctgattgagc aggctgttgc ggtttacatc gtacagcgcg gcccagttga tctgactgat
     3121 gttggggttg ttgcgaaaat cttcggccac ctgtccgcct tcttcggtgc tctgctggtt
     3181 cacatagctg ggcaggtagc ggtagtagct ggggcgcgga tcgggtgcgt caaaccagtc
     3241 caaaccggtg ttcccgtctt tgcctatttg catcgaggcc gccgtgatga ggactgcatt
     3301 cttgtgtatg ttccagtcgt ggcgaagcat aaacaccggg cgttgtgtgc gcacttcgct
     3361 ggagttgcgc ttttcgccgt tttggaaccc ccacagcggg ttgtaaaaat tggtgcccgt
     3421 cagatcatac aattcactca cactgctggt ggaacggccc ctgcgaatgg gcgcgtcgaa
     3481 ggccgtgaag ttgagtgagt ggtctttgtc aatttttttg tcaacagaca ggaaataggc
     3541 ccagccgtcg aaaaaagtgc cttccacata gccctcctgc gcccaacgcc gtgaaccgga
     3601 aagcgtaaat gcccagccgt tttgcatcaa acccgtggac caggtgccca tgatgcgatt
     3661 gcgaaaagtg ccgnnnnnnn nnntcgacaa ggagtacgac aggcgcaatt gtttgcggtg
     3721 gaaagaggca cgggtatcta tggcattgcc gccgcccacg ccgccgaaag tgtaggaaat
     3781 agggcctgtg ccgaggtcca cctcgcggtt gcgggtcacg tcgttgaggc cgccccacac
     3841 gttccaggtc acacggccat tatccaagtc attgaacggc agtccattaa gaaatacgtt
     3901 gacatactcc gaatcgtaac cgcgcacccg gaaacgggcc gggccgaaag taaaagccgc
     3961 cgccgataca aacacatcgc gcgaggccgt gagaatgccg gagatgtttt ggttattgcc
     4021 ctccacttcg aggttgtcgt ccgacagcga gatggtggga atgaggtctt ccttcagcgc
     4081 cacgcctgct tcgcctcgcg gcgccagccc tatggtactc aggtttaagt ctttacctgc
     4141 tatagataca tccaggttgt actctacaaa attgtccaat cggatattga gcttgtaagc
     4201 tcccgcagga atgttctgta aagaaaaccg tccctgttca tcgctcaacg ttgagaacga
     4261 agtgccctgc agtgctacca ccgcgccttg tgcgggttgt tggttgcttt ggtcgagcac
     4321 gacgcccgta atgcgggcat tttgagcgga agctgtggaa aggaaggaga taaaaaatgc
     4381 gttcagaacg aacgcccaag cgaggtgacg cagcatgttg gccggtgttg gtgaaaaaag
     4441 cggctcaaag gtaatgcttt ccacaggaaa atgcgcagcg ggggaaaaat taacatcctg
     4501 gtaaattact ttatcgccgg aactgcgtta gtttgtataa aatttggaat tcatgaaaaa
     4561 ccatctgttc gttttagctt tcattttgct gcccctggcg gctttctcac aacccaaaca
     4621 atacaaaatc gccgccatcg gattttacaa cctcgaaaac ctcttcgata ccatcaacaa
     4681 tccgctgacc aacgacgaag acttcctccc cacaggctct tacagctaca ccaccgccgt
     4741 ttatgttgaa aaacaagcca atatggcccg cgtcatcgcc gatatgggca ccgaactcag
     4801 ccccgatgga gtggccattc tcggcgtggc agaggtagaa aaccgcaaag tactcgaaga
     4861 cctcgtggca cagcccacca tcaaagaccg caaataccag atcgtccact acgactctcc
     4921 cgacccgcgc ggtattgacg tggggctgct ctatcaggcc aaatactata ccgtcacagg
     4981 cagcaaagcc atccctatgg tggcttatga agagggcggc gaagcccgtg gcacccgcga
     5041 catcctcttg gtcagcggta tcatggacgg cgatccgctg catgtcatcg tcggtcactg
     5101 gccctcgcgc cgcggcggcg aggccgctac gcgccccctg cgcaatgcgg ctgctctgat
     5161 ctgcaaaaac gtagccgatt ccatcacctt agccgatccc aacgctcgcg tcatcatcat
     5221 gggtgacctc aacgacgatc ccgttagccc cagcgtgcgc caggtgctca acgccaaagc
     5281 caaaaaagaa gatacacctg accgcggctt cttcaatccg ttttacgatt tttataaaaa
     5341 aggcatcggc acattggcct atcaggattc ctggaattta ttcgatcagg ttattctctc
     5401 ccaaagctta gtgaatccta aactcggcgg ctaccagtac tatcaggccc gcgtattcaa
     5461 tgcgccctac ctctaccagc aaaccggcca cttcaaaggc tacccgcacc gcaccttctc
     5521 cggcagcacc tacatcggcg gttacagcga ccactttccc acctttgtgt atctcgttaa
     5581 ggaaatttcc gggaagtagt taggttttgg gttggaacgg gtggctgctg gctactggct
     5641 tctacgggtt ggaacggctg ctggctgccg gctacaagct gctggccgcg tttccctcgc
     5701 gcttagcact tgcccaccga agactgccta ccgcttaccg ataccccctc gcattcgcgg
     5761 aatttcgcga tcttcgctaa agttcgcgtt aaaaaaaacg catttcgggt tcaaggtttc
     5821 gcgttcctct cgcattcgcg gaaattcgca cccttcgtta aaattcgcgt taactattcg
     5881 cgtttcgggt tataggtttc ggggtgctcg ctcctcacac actcacccct cactcctgaa
     5941 aaccgcccca accctccaac aaccgcgccc tcagcctcgc tttcacttcc tccggtatga
     6001 acgcatggcg cagcgcctct aagttgcact gcaacagccg ctccttttgc cagccgaata
     6061 cccgccgcat ggtatcgtat tcctgcgtca gcgttacatc cgacagcgct cgcccatccg
     6121 tgttgatgct catagaaacg ccgttgcgcc atattttttc tgccggatga tcttctatcg
     6181 tcgccactac attggtttgc acattgctgg taggacacag ctccagatgt atgtcttccg
     6241 atttcaaaaa agccagcaga gcctcgtctt ccgcactgcg caccccatgc cctattctgg
     6301 ccggatgaaa atgctgcagc gtttcccaca cactttccgg gcctttggcc tcgcctgcat
     6361 gagccgttac atgtatgcct ttttcgcggg cgtaggcaaa agcgctgatg tggttgtcta
     6421 tcggaaagcc ggcctcatcg gcggcaatgt cgaaacctac taccaatgtg cctgcaaact
     6481 gctcggtcaa acgcaccgtt tccatgcttt gagcctccga aaaatttcgc aacgtacaca
     6541 gtatcagccg tgcctccacg cccgtttgag ccatgccttc cgccagcgcc gcttccaccg
     6601 tttgtaccac ctctgccggc gccaagccct gctgcgtatg caacaaaggc gcaaaacgga
     6661 cttcggcata gatcgccccg tcctcgcgga gttgttccat gagatccaac gtcacccagc
     6721 gcagatgctc gcgggtttgc atcaggctaa tggcactgac ggcacgggag atataatcgg
     6781 ccagatcggt gcatttggag ggcgccacat agtcgcggcg ataggtttcc cagtcggtat
     6841 ccggcttaat tttgcgcact acgtcaaaac tcagggagca gtccaaatga agatggagtt
     6901 caattttggg caaatgaagg aattcgctcg tcacggtagt tcttgtttct tattgtaaac
     6961 aggaaaacgg gtatcgaaca agaccagcct aagccgacag cccgataccc gtcatcaaca
     7021 atcttacgct tttttcacgg cctcgtcgcg cagttcgcgg cggaggattt tacccacatt
     7081 ggttttgggc aattccgtgc ggaactccac cgacttgggc attttgtatc cggtgagatt
     7141 ttcgcggcag aaggccagca gttcgctttc ggtgagcgat gcgtcctttt ttaccacaaa
     7201 aattttgacc acctcgcctg atttttcatc gggcaaacca atggcggcgg cctccaatac
     7261 cttcggatgg ctcaccacta cttcctctat ttcattggga tacacattga aacccgaaac
     7321 gagaatcatg tcttttttgc gatccacaat gcggaagtag ccgtctggct ccatcacgcc
     7381 gatgtcgccg gtacagagcc agccttcgcg aatggtgtcg gctgtggcat ccgggcggtt
     7441 gtagtagccc ttcattacct gcgggccgcg gcactggatt tcgccaattt ctcctaccga
     7501 cagtaccttg cctgtttcgt ctatgatacg cacgtcggtg gaggggaacg gcaaaccaat
     7561 ggtaccgagg cgaccgctgc cgtcaaaggg attggccgta atcaccggcg aggcttccgt
     7621 cataccaaaa ccttcgctca ggaagcagcc cgtcacttgc tgccagcgct cggccacggg
     7681 tttttgcacc gccataccgc cgcctaccgt gatcttgagt cggctgaaat cgcagcttgc
     7741 aaaatcgggg tgattgagca gggcattgaa cagcgtattc acccccgaca ccaatgacac
     7801 cgggcgggct ttcatgattt tgatgagcga aggaatgtcg cgggcattgg taaccaatac
     7861 attctcagca ccttgcgaca tgagcgccag gcagttgacc gagaaggcaa aaatgtgata
     7921 caatggcaac ggacacaaag ccacctcttc gccctctttg aggaagggct gcatccaggc
     7981 tttcatctgc accatattgg ccacgaggtt gcggttggtg agcatggcgc ctttcgatac
     8041 gccggtggtg ccgcccgtgt attgcagcaa aatcacatcg tcggaattgc cctgaaactg
     8101 cggcagactg aatttgcgcc cgccttccat cgcctgcgtg aaggaaacag tgttgggcag
     8161 ggtaaatttg ggcaccatct ttttgatgga gcgcaccacg aagttgacga tggcgccttt
     8221 tgggaaaccc agcatctcgc cgatgctcgt caaaatgatg gttttgatct gcgtctgact
     8281 gaggattttc tccagattgg aagcaaaatt ctcagcaatg atgattgcct tggccccgga
     8341 atcattgaac tgatggagca tctcgcgggg cgtgtagagc gggttggtat tgacaatgac
     8401 caaaccggcc cgcaatgcgc cgaacaatgc aatggggtac tgcgcgaggt tgggcatcat
     8461 cagcgcaatg cggtcgccgg gctgcaaacc tctggaatgg aggtaagcgc cgaactgcgt
     8521 agagagttgg tccacctcgg cgaaggaaag cgttttgccc atacaggaaa acgcgggccg
     8581 gtttttgaat tttttgaacg tctcgtccag catagccgtg agcgtcgggt aggaatctgc
     8641 gtcaatattg gccggtacgc ctttggggta caaagagagc catggttttt cgtctgccat
     8701 aattgcctgg ttttaatgta tgatatttgg ttggttgatg tcctgatgtg cgatgttgca
     8761 gcacaaagca atacgatgct gcaaaggcca acaaaactat gcaaaatccg ataaatcgca
     8821 agcaaaagcc cgaaaacctt tacaaaagag aaaaaaatcg gacttttgtg catgaacaac
     8881 ggccaatgcg ctgtgttcgt catggaacat ctcaccattc aataaaacga aaataccatg
     8941 ctgtatcaaa ttctcgacca cgcacattcc gggtttcgct gggtagtgct tttgctcttg
     9001 ctggcggcta ttttttctgc ttttcaggct tggcagagcg gccgtgcagg cgcttccaaa
     9061 atgtcgtttt acgccctgat ggccgcgcac attcagttgc tgggcgggct tattttgtat
     9121 ttcatcagtc cgtacgtcaa atttgaaggc ggcgtgatga aagacgccgt agctcgtttt
     9181 tacactgtcg agcacatcag tatgatgctt attgccatcg cgctcatcac ggtggggtac
     9241 agcaaggcca aacgcgccgc caacgatgcc gcccgcggca aaaccacctt ctggttctac
     9301 ctcatcggcc tgctcgtcat tctggcggcc attccctggc ccttccgcag cggactgggc
     9361 gggaactggt tttgaggaac tcatcgaaat caggctgcaa gccacaagcc cttagctaca
     9421 agcaatgaac tgcaagcctt cggaatcaca tcctgagact tgcagttcat aattttttgt
     9481 gcaacagcac catatcacga taggagcttg aggctaacag cttgcagctt gtggcaaaca
     9541 gctaaagccc ccgattatca cctccttacc agcagcttgc ccgaagctac cgtttgcccc
     9601 ccggaaacga tctggaaaaa gtacaaaccc tgcctcatgc ccgaagcatt ccacgttgtc
     9661 tggtcgccgc tgaagggttg cgtgccgacc aactgcccct gtgcgttcca cacctgaagc
     9721 aaaccccgct cgatgccgcc cgaactgagc ctgaactgcg ccgaatgcgc cgccggattg
     9781 gggaataccg tgagttccaa tcccggccgc agcgctacat cttttacatc caccgtaatg
     9841 aggaagtttt cctgcacggt atgcagcgta gtgttggtaa tgaccggctc gttgaagtcg
     9901 aaatagatgc cggcgctgtt gcggatggcc gttcccagcg gcacatcggg gcgctgccgg
     9961 atggagaagc gcacaaaacc atgcgaagcc ggttcattca cgttgctgtc gggcagcatg
    10021 atattggaaa acacgaactg gatggcgttt tcatcagaaa aattgaacgc gtagggatgg
    10081 ctggatgtgc cgggccgaat ggacgccgga tcgagccatt cgctcaatgt gtcgcggatg
    10141 atgaccgtaa aagccgtgtc ggtgccggtg ttttggaagc ggatgaggta gtcgagcggc
    10201 tggccaggcc ggatgaagcc ctgttcgccc acgcccagcg ggaagccttg tttgtcgttg
    10261 gggtcgtaag cgccgatatt gtccctgcaa tccaggtcgt agttgggctt ttcatcgccc
    10321 agggcaaact ggttgataaa accgaaactc gattcgccgg ttccgcagcc ctccacgaaa
    10381 gccgcctgat agttgttgtc ggggtgaaat gctacctgtc ggagttccaa acgccaggta
    10441 gaaccgttgg ccggcaattc cacctgcctc atctggccgg aaccgagcag caaatcatcc
    10501 tccataattt ggatcataat atcctctatc acaatgtatt ccacagggtc gttcatatcc
    10561 tccccctcat tggtaatggt aaacaccacc cggtcgccct gacattcgcc ttctacccgc
    10621 aagctcgccc ccgaccagtt ggggtcaatc atacaaggcg ctaccggaaa catctctgcg
    10681 ctgatgcagt gcgtctggcc cagttctgct tcacaactca ccgatatggt gacggtaaat
    10741 gtgcgggttt caaacacatc caaatcgccc acatcaaacc tgtaagtgct gccgtttacc
    10801 gacgctacag gcaacgagct tcctacgaaa gtaaaaaacg gatcgagcgt gacgttgaca
    10861 taggcatcct cgatgagttc ggatccctga ttggtgtatc gaaccgtgta agtattttca
    10921 acacagcgac gcagaaatgg tatgccgatg ttgacttccg gctgtgggca atcgatatcg
    10981 gagcgcacgc cgaagttcac accgagggcc tgttccatag attcggcaaa aactaccggc
    11041 tgtgcattgt tgcattggat attccagacg ttgctggagg gcaaaaccac gctcactgtg
    11101 taacttccgg gcggcacgtt gatgcggtat tcgccgttgg gcaatgtgga gccnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnntca ccttgacaat tgtcgtttgc atcatgaaaa acccggcccc gaatgctgga
    11281 aaaatgaacc tcctggatgc gaagcgaagt aagagaggtg ctgttcacat ctcctgtttt
    11341 gacggccaca aaattgactc ctgtaacatc ggctgaaaat ttactgacga ccaccgaccc
    11401 cggataggcc agcgacaaag gattggccgg attgggaaac tgataggcgg cgtccacaaa
    11461 tcgccaggag ctgttgttgg gaaattcctc tgtgactccc agaatcaaat tcctcaaatc
    11521 aaaagtgtcc agcgcattga tgatgctgtt gcgatccaca tccgcagcaa tgaattggta
    11581 cggagacgtg aatggttccg taccttcaat atgacgtacg atgagtacgg catctaatgt
    11641 gctgacgcca ttgagcgggt cgaggtcgta ttcggggcga acgacatagc tgccgccgac
    11701 aggtacttgc acagcataat tgccgttttc atcggtcagg gtttgggcgt tgacctggcc
    11761 ggtgacgaaa atcgttacgc cggaaactga ttcattgttt cgggtggcaa tccgaccgct
    11821 gatggtggct tgctgcgccg acaaagacac agaaacggcc atcagcatca gcccggcaat
    11881 gaggcgaaac atggtgtaga tagcagacat gggatgattt tttttggtga tgaaataaaa
    11941 gaacgatgca aaaatgagcc ggatatgccg ccggggcaat gacaaattta gaaatttgtc
    12001 tattctttta ttttaaaagt acaaacataa actctttatt atcaaaggct attttaaata
    12061 tctttgtcta ttcaaaattg ccgtttctca tcatggaaaa cacccgattg tggcagcttt
    12121 tgctcacttt gacgccggaa gaacggcagg gcttgaaaaa atttgtggaa agcccctttt
    12181 tcaatcagcg cccggaagcc gcccgcctgc tcgatgcact gttgatttct ttcaaaaaag
    12241 gccgcccggc acccggcaaa gagcaggttt ttgagcaggt ttttgaaggc cgcgatttcg
    12301 acgatcaccg gctgcgcatg gccatcagtt ttttgtatca gttggcccgg cagtttctca
    12361 ccgtgcagga ttttttggag gatgaagtgc atgtcgccat ccgcgaatcg gaggtgctgc
    12421 gccgccggaa actctatgcc ctgtctcgtc attgcttaga ttcagcccgt aatacactgg
    12481 atgtgcataa ggtgcgcaac gccgatttct tcgatcagga ataccggctg ctgctggaag
    12541 aatgtcgttt ggaaatgagc cgccccggcg cccaacccga acggcttcaa gcgctttcag
    12601 agcagttgga caaggcgttt ctcagccgga aattgtggca aaactgcgcc ctgctctctc
    12661 atcaaaacat ggccaaatcc gatttcggcc ccggcttgct ggaccacgcc ctcgacggct
    12721 tagacgaacc cgctgtgcct gccatcgcca tatatttctg gtactatcgc gccctgacgc
    12781 aacccgaaaa cagcgagcaa cactaccagc gattcaaaaa aatgctgttt gaacacgccg
    12841 atattttccc cggcgaagaa ctgcgcgacc tctatctgct ggccatcaat ttttgtatca
    12901 aaaagtacaa tgccggcaat caggcttttc tgcgcgaaca attggagttg tatcagcggg
    12961 ggctggaaca caggtacttt ctggtggccg gcgaactgtc ggcttacacc tacctcaatg
    13021 tggtgacgct ggccttagta ttgcaggagt tggactgggc cgcgcagttc atcgaggcgt
    13081 accggcccat gctggcgccc gaaaggcagg agagtttttt cagtttcaac aaggcgcgcc
    13141 tcgaatacgc ccaaaaacgg cccggccatg ccctgcaact attgcaaaaa gcagagtata
    13201 aggaggtttt gctcggattg gcggccaaaa ccctgcaact caaaatctat tacgaatcgg
    13261 gcgagtttga cctgcttgac gcgcacctgc aaagcctcaa aacctatttg taccgaaaaa
    13321 aggtgatggg ttaccaccgc gaaaactata tgaacaccat acgttttnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcagt
    13501 tgtgacagac agcccctcac ttccacgcat aactcacatt cagttccgcc gaattgccca
    13561 agctccgccc cgcgccatgc gtcaggccca catcaaaacc gaagcctatt ttcagttgcc
    13621 catcggctat gcgcacctgt tcgcccagca gcaccccggc ctcagcgtgc agcgtcatac
    13681 ccggcgcctg ccccatcgaa acattcagcc ccgtgcccgc ccacaccaat tcgctcactt
    13741 gcagtcgcac attggcatcg gcattcaggc cgccgcccgg cgcatatttc agccacagcg
    13801 aaggctcggc aaaggaggtg gtattgccca accagggcgt atccaaatac ccgcccagca
    13861 ccaaataagc gtgcggtacc cgtcgtatgc cgatctcgaa attttcctgc ctgaacgggg
    13921 taaaaaaacc gaaagtttgc ggcacggaaa tgcccacata gtacttgtcg gaatgttggt
    13981 aaaacgcgcc catcccgatg tcggggcgca gggtcgtcac attgctcagc gctacatctg
    14041 ccggattggg aaactcaatt tcgctcactt tggcccggta ctgtaccaag cccgcgtgca
    14101 gccctatcgt gagggtatgc gtcgtgcgcc gccccatttc catgcggtag gcgtagcgcc
    14161 catacacgcc cgtctggcct attttccccg tgcggtcgtt cagcagagcc aggccccaca
    14221 cgctgttgaa tttctcctgc acccactccc agttgagcca ttgcgtgcgc ggcgattccg
    14281 gcacattcca ccactgctcg cgccaggtag cgctcagggt catggtgcga ttgttcagca
    14341 cataatgccc cgacaaagcc gccggattca gcaggctcca ttggtctcga tacaccgtaa
    14401 aaatcggcgt ctgctgggct gccaattccg ttgctccgac acaaagtgca gtacacaaaa
    14461 agcacatttt caatatctta taatttttca tagacggtgt tttttttacc tgaacaaatg
    14521 caccaccccg cgctgtgcct cgcccacctg cacattgccg tcaaaggccc gcaacacata
    14581 ataatacgcc ccttccggca gggcattccc gctcctgtgc gcatggcccc gccagggatt
    14641 cccgttgtcg tattgttttt cttcaaaaac cagatcgccc caacgactga aaatctgcat
    14701 ttccacccgc gaaaaaccat tcaaaccttt gatggtcaat tgcccgttca gtccggggtc
    14761 gtccacgatc agaccgcccg gaactgccgg catgttcagc acttctatcg tcacggtagc
    14821 cgtatcgcag gccgtgccac agcgcgcacc gggattgcac accacatact ggaagctcac
    14881 ccgcccgcga aaatcttccg tcactgtgta tcgaaacctg cgcccgtcca acaactccaa
    14941 ctgccccacc gccggccctc cgaggtacga aagcaccgtg tccggcagcc cgctcaggtt
    15001 gtcgttcagc agcacctcca tcaccagcac atcctgcacg cgctgcaagg tgtagcggtc
    15061 gtcgttcagt acaggcggcg cttccacggt caccgtcacg gtatcggctg ccgaaaaagc
    15121 gcaaccttcc aaactcacct gccatacaaa gcgattgggg ccgcgctgca nnnnnnnnnn
    15181 cgcgcaagcg tctgttcgct cgccgcattg tccaccgtag ctgcgttcaa gggcagccac
    15241 cttccggttc cgcccagagc cggcaaagcc cgcagcgtca ccgtaccggc agcgcacaat
    15301 accgtgtcca acatggccac cgtagccgca tcatcgggca gactctgcac ctgcaccggc
    15361 gcgccgccca cgcgaatgga acggcagccc gatgaatcgc gcaaagcaaa atactgcccg
    15421 ccataagcgc cggaagctgc cagtatgccc accctcggca gcacggttgt cagcgaatcg
    15481 ccgttgggaa tgaaccacac atagcgcaca ttcggcgagg ggtagaggtc atcggcctcc
    15541 aagcggatgg tatcgccctg acaaacgcgg gccggcgccg ccactgcgta aacgggctgc
    15601 gccacgcaag ctcctccccc accctgtccg gctgccacgc tcgcgctgcc gctgatggta
    15661 cactgattac cgtccgtcac cgttacgctg taatttcccg gcgctaatcc gctgatagta
    15721 ggcgtattac ctccgttgtt ccatgcaaaa gtatagccgc ccgtgccgcc ggttgcagcc
    15781 gcagtagccg tgccgtcgtt gacgccggca gtggtttcag gcgttgcact tacattcagt
    15841 tgaagcgcag gcggatcggc gaggttgatg ctccgggtag ccgtgcagcc gcccgcgtcg
    15901 gtcacggtca cgctgtaggt tccggcgatt agtcctgtgg gattttgctg cccgtccaa
//
LOCUS       DAORLF010000042        15923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1979_length_15923_cov_2.129632, whole genome shotgun sequence.
ACCESSION   DAORLF010000042 DAORLF010000000
VERSION     DAORLF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15923
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_15923_cov_2.129632"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>217)
                     /locus_tag="PK198_05800"
     CDS             complement(<1..>217)
                     /locus_tag="PK198_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38277.1"
                     /translation="LFAHPAIDIALPISSPSDPVLCKPGNIIEVQVANTVFNNGYQLY
                     RNGNPQAFAFQNGNGGTIQFTTESFDST"
     assembly_gap    218..310
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..563)
                     /locus_tag="PK198_05805"
     CDS             complement(<311..563)
                     /locus_tag="PK198_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38278.1"
                     /translation="MSDATYIVKLRAIRGDQAVEVERSLYVGYQPTTADYAWVTFQTP
                     QPACYGQSAQVFTNYSFTDQLQLRQGNEVVAGPFSINSNA"
     assembly_gap    670..718
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<719..1551)
                     /locus_tag="PK198_05810"
     CDS             complement(<719..1551)
                     /locus_tag="PK198_05810"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRF38279.1"
                     /translation="MDAGQSWVKAYEQGELTGLEVYIFNDLFFTNEQRGFAVGYDFFG
                     ADYLILRTTDGGSSWSPYTYEFGFNIGQCWSLDFLDHQNGYVVGDWGRIFKTTDGGTN
                     WLPLGTPSNTRLTDVDFVSPQLGWVLSIEGALMKTTDGAGTWQVQSTPVEFNRIQFLN
                     ADLGFALADSRFFKTTDGGANWLPVSEPLPAGTVEFQFIDSQNGFSFVGNRAIITNDG
                     GQTWFYQQVTPNLASGDFGGFNDISILNEHVGYMAGFQNNDGVVNTLLAQTENGGGIG
                     L"
     assembly_gap    1574..1625
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1972..>4042
                     /locus_tag="PK198_05815"
     CDS             1972..>4042
                     /locus_tag="PK198_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38280.1"
                     /translation="MRFYFGLAQSCALLFSLSAFIATAQTTQCGITVNAGSDQVFCTP
                     GQMLTLNGAVTGDFLSAAWSPTTGIANANSLNTTLTANAAGNYTLTARTVSGPNLVVN
                     GDFSQGNTGFTTQYTLGSSGQFGPLTNTGTYGITTVANLLHSQFAPCSDHTGGGQMLV
                     VNGATTPRNMWCQTVTVQANTDYAFSAWFAVVATQNPSLMRFTINGNTIGNNFTLPNV
                     TCEWTRFFRVWNSGAATSAQICIANVSTSAAGNDFCLDDISLRTVCDVADVIAIAPAN
                     LNAGFQTNPVCANSAAFDLNTLLSPGSTAGGQWTLNGAPATTFQPAVLGAGTYMLNYS
                     VQQGSCSRDSTRSITVHPLPDAGTAAQPAQVCRGTVQTLMLSQLLIGANAGGNWAETS
                     PLPSPPGAFNAGAGSLQTQSLTTGQYTFRYIAAGTAPCPNDTTTVSVVITAPPVAQAG
                     PDQTLDCVMRQVSIGSSASSSGPGIVYNWSSPTGGSVAQPTAAITDTETAGTFVLAVT
                     DNNTGCSARDTVMVASLITNPVFSLDIQPVTCHGDNDGSISVSNVSGAADPAMFQLNG
                     GTAQTTGIFNNLPSGNYTLRLTDANGCFAERQAILPEPPPIMLELGSDLSGTPAVLPL
                     GDSTTLRILTNLAPDQIQSAIWLPIIPDCSNCLTATVRPVEKTTYIVILTDANNCVDS
                     ASLTIVID"
     assembly_gap    4043..4197
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4360..4782)
                     /locus_tag="PK198_05820"
     CDS             complement(4360..4782)
                     /locus_tag="PK198_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRF38281.1"
                     /translation="MADRLKVVIDTNIIVRSISGKSLASFVFDALLKQEFMLCLSTEI
                     LLEYEEKLSQIYDQETAELVMSVLLMLPNVEKVNIYFDFRLIAPDADDDKFINCMVSA
                     NADYLVSDDRHFRVLSRIQFPKIECLTYEDFKRILPGS"
     gene            complement(4772..4990)
                     /locus_tag="PK198_05825"
     CDS             complement(4772..4990)
                     /locus_tag="PK198_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38282.1"
                     /translation="MSVEKRTAPLSNLQIELLKMYSAGVPDQYLEDLKILIARFLFAK
                     ARAKADQLWDEKKYTDELLHELLKRNGG"
     gene            5082..5672
                     /locus_tag="PK198_05830"
     CDS             5082..5672
                     /locus_tag="PK198_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HRF38283.1"
                     /translation="MNFLAHLLLTHDDESLLIGNFIGDHVRNHELPQYPVGVQQGVWL
                     HRRIDTFTDNHPAVRECLKLLRASHGKYAGVVWDVLSDALLSHNWDRFHDASLEVFTA
                     RMYEVLERNLHHIPEALRKRVPLMIADNWLMKYAREEGIEFTFSRLQLRASQPEWLNG
                     TTESLRLHYAALEEGFLQFFPELMTHIATLRSEMKA"
     gene            complement(5644..6390)
                     /locus_tag="PK198_05835"
     CDS             complement(5644..6390)
                     /locus_tag="PK198_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF38284.1"
                     /translation="MHTSHTGHLFHLFGSKIVLPAFLLALAVTGLQAQYSGGGNYNYL
                     DFNQKPYYFGITLAINNSSYKPFRSAGFLESDSITRVESATGPGFNLGIITNLKIGEH
                     FDFRFMPTLSFAERNITYDRRGRQATFTQRRVESVLVETPFHLRYKSAPYRDMRLFVV
                     AGVKYAFDVASDSRARNSENLVRIAPTDFSLEYGAGIQFYLPYFIFSPEIKFSHGIGN
                     VLLYNRSLQESTVLEKLLSRGFTLSFHFEG"
     gene            complement(6339..7061)
                     /gene="ubiE"
                     /locus_tag="PK198_05840"
     CDS             complement(6339..7061)
                     /gene="ubiE"
                     /locus_tag="PK198_05840"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRF38285.1"
                     /translation="MEVKPYSSEGSKKSQVSAMFNRIAPYYDLLNRVLSLGIDRNWRK
                     KAIKLLESKQPKHILDVATGTADVALETARRISPDSIIGVDISADMLAIGRQKISKQG
                     LDEVIRLEEADSENLPFADNTFDAVTVAFGVRNFEHLELGLAEMRRVLRPGGQVVILE
                     FSKPVAFPFKQLFHFYFRNILPFIGKMTSKDPKAYRYLYESVQAFPDGDAFLEVLQKT
                     GYNNNRCIPLTLGICSIYSGQK"
     gene            complement(7153..8358)
                     /locus_tag="PK198_05845"
     CDS             complement(7153..8358)
                     /locus_tag="PK198_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF38286.1"
                     /translation="MLTRTVHLYRNSFHGLTRDMWLLSLVTFINRAGTMVIPFMTVYL
                     TQELQFTKWQAGLVMSFFGVGSILGTMAGGKLSDRHGYYPVMFWSLFSTGFMFLLLMF
                     MRSFWAISVTIFFLSIIGEAFRPANTSSVAAYSVPETRTRSISLLRLAVNLGFSIGPA
                     LGGFLAHSLGYNWLFLADGITCIGAALLFRFMLKPKAAGTPGSKPVVERPALVASPYR
                     DVPYLVFAAMITLWAVAFMQFFSVIPVYFKEHFLLNEGQIGALLALNGLLIALFEMPF
                     VYSLEGRYKHLGLIRWGIVAVAVSYLLFLAPTHWVMLAVVAVIVITLGEMLAMPFASS
                     FSMNRSNDFNRGQYMAVYSLSYSVAHVAAPLVGMNVADHFGYGALWVLLAGLCGLSYA
                     GMMWLAKRA"
     gene            8567..9004
                     /gene="crcB"
                     /locus_tag="PK198_05850"
     CDS             8567..9004
                     /gene="crcB"
                     /locus_tag="PK198_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRF38287.1"
                     /translation="MNYLLIGAGSCIGGMLRFGLSQWIQTRTEAVFPYGTLCINLLGC
                     FLIGLVFGWSERTAISQEWRLLLTTGLLGGFTTFSAFSNETVGMLRSGWYAGAVLYVL
                     GSVLPGIAATFGGYWVAKAGGGDFKYLQLLLGVNSKVLLEVIA"
     assembly_gap    9049..9081
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9082..9337)
                     /locus_tag="PK198_05855"
     CDS             complement(<9082..9337)
                     /locus_tag="PK198_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38288.1"
                     /translation="MKSNFGKIFENIVYYLIFLPGLIYLSWASMRDWVLELSDPHSTV
                     FLFEKKMLVPYMILSLYHWIITVLLFYWGIIKKIGITNKEF"
     gene            complement(9403..9747)
                     /locus_tag="PK198_05860"
     CDS             complement(9403..9747)
                     /locus_tag="PK198_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38289.1"
                     /translation="MENMISRWLTNIIYFGLFLSTMIIVSWLMLNPYEGGLWSAFRAS
                     NFPKKDLYFSIFFSLYFLSSTFLLLYWGFRKKHGVTMSVFVWSFVLWLIAIALILIIL
                     LRAFSSIPVGMD"
     gene            complement(9779..>10434)
                     /locus_tag="PK198_05865"
     CDS             complement(9779..>10434)
                     /locus_tag="PK198_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRF38290.1"
                     /translation="SESLLAREGAVLLLYGREPERISFLNGVKQSAWSLLHLLRENNW
                     QVLATEQPVEGEFEGTALHGRADLVLQRGDELAVLDLKWRGIGYREDSIRNEADLQLV
                     LYARLVTGSQGWAHSAFFIMEKARLLARNNAAFEKITPVRPGSDHIEANERILRKMQA
                     TYRWRLNQLLQGDIEVRCQQTRSELEETYSGQLLDLLEMKSDDARYDDYRVLINLVE"
     assembly_gap    10435..10571
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10572..>11793)
                     /locus_tag="PK198_05870"
     CDS             complement(<10572..>11793)
                     /locus_tag="PK198_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38291.1"
                     /translation="TLLRRNPDFRPLCYISDRSRSLDNALIQEGLPSLGLLSASQARP
                     TLQVLKLAAGFLWNPVDPFKLMEFVSLAVKPVEDGLAAAIAESLAQTPGLHGEGWRIA
                     VARYFDEAEQLAAQNGGPDSAKARKQYRFWFERKRYDSSRSAPKADAVEIFRYIEQWA
                     KESMDENMLSNSTLPALREQARRIVELLESLPETQLGYLELERVVRTVYQSSPLQLSP
                     REQGHLPFVTRPGALIEPVEELLWWNFIQKEPDHFFSRWYRAELAWMQARGLEVEGPA
                     LQNERLLWQRRRPVMAARKRLLLLLPHSSEGEEAPPHPLFGDLSAAFSGIDRITLDVD
                     ADTAGDAWKTGPVLPQKTALPRRYLGRPRPFLHILPHRRVEAREKETFTSLETLLYYP
                     YQWVFRHKIKLVKSS"
     assembly_gap    11794..11830
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11831..12516)
                     /locus_tag="PK198_05875"
     CDS             complement(<11831..12516)
                     /locus_tag="PK198_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38292.1"
                     /translation="MLLYFGLALEEEPWRMPAPPWSGMFWVGPQGLLNVLESHLGLAG
                     AQPLDEFLRIEQYRQLLSAHLSEHPDAFFAESFAADPLATATALLAYRDELLLAGWDF
                     ALSGDEPPRVRVLAELETALRIGVEEGKAFSPGWADRFSAVLSVLSTRRHPVQEIKIV
                     EPPELLPPHFRRLLAALEKTGVQLTWLPQIQPSDADTDLARFQRVLAGQGTGKTELRG
                     DGSLLLARTRR"
     gene            12564..13172
                     /locus_tag="PK198_05880"
     CDS             12564..13172
                     /locus_tag="PK198_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF38293.1"
                     /translation="MSEIFMLETIQHWDQQVFELLNGQWQSPLLDWLAPLWRDKRFWI
                     PLYLLLSIFVVRTYRKRGLYFLLALGLTAGISDVASSRIVKPSVQRLRPCNNPELRDD
                     VHLLVHCGSGYSFTSSHAANHFAAAAFLFFTLGRLHRRGRGLWWLWAASIAYAQVYVG
                     VHYPADILAGAVLGAAIGYLSSRLYLRLPNLSIASMNTNQRA"
     gene            13169..13903
                     /locus_tag="PK198_05885"
     CDS             13169..13903
                     /locus_tag="PK198_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRF38294.1"
                     /translation="MSISQYLLLFFAVILGGVIGFGLQKYERNHLKIVLAFSGAFLLG
                     ISVLHLMPAVYVQQKEQAGLWILLGFFIQLALEQLSTGVEHGHVHAHPHAPRGFAIQV
                     MIGLCLHAFLEGMPLSGYEQVHTAHDHSGHAEHHLLYGVVLHKAPAAFALVMLLLSSQ
                     FKKAAVICCLLVFASMSPLGALLTQFIGVNQGTFSILLAVVIGSFLHIATTILFESGE
                     SKHHRVSWGKLAAIVVGVALSMLTML"
     gene            14041..>15562
                     /locus_tag="PK198_05890"
     CDS             14041..>15562
                     /locus_tag="PK198_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF38295.1"
                     /translation="MILIFFISIAGFSANAQATLRGDVTDAGNSDPLIGASVVIKGTT
                     EGTVTEYDGTFELKTKQPLPLTLIISYVGYNEIELPVNAANQPLKIKLNASAITTEVV
                     EIKGQRISEKQKANPLTVESLDLIAIKQAATDNFYDGLGSLKGVDLTAASLGFKIINT
                     RGFNSTSPVRSLQIIDGVDNQAPGLNFSLGNFLGSSELDVVKVDLIVGASSAFYGPNA
                     FNGVISMETKNPFLHKGLSSSVKAGERSLLEGAFRWADALKNKNGHDAFAYKVNFSYM
                     RAYDWEADNYDPVDGTDTGVDNPGRYDAVNVYGDEYFTGFDYSTFDLHTYPGLGAWHR
                     TGYREKDLVDYNTRNIKANAAFHLRTNPAAGDASPELIYSSNFGTGTTVYQGDNRFSL
                     RNIRFFQNRLEFRKRDQYFIRAYMSKEDAGDSYDPYFTALLLQDSAKRNTDWGIDYVD
                     YWLRNVAPVIKNAEGFPRLNIVFDPNTGQVTATFDREAALAWLANNKDRIDAWHTQA"
     assembly_gap    15563..15691
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15692..>15923
                     /locus_tag="PK198_05895"
     CDS             <15692..>15923
                     /locus_tag="PK198_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38296.1"
                     /translation="RFIDRPALYHVHGEYKFKPSFLEYLTVGGNARLYTPVSEGTVFY
                     DTAGIRLTNFEFGMYSGLEKALFDNRLKASLAL"
BASE COUNT         3784 a         4154 c         3821 g         3479 t
ORIGIN      
        1 gttgaatcaa aggattcggt tgtaaattgg atggttcctc cgtttccgtt ttgaaacgca
       61 aaggcttgcg gattaccatt gcggtacagt tgataaccgt tattaaaaac cgtattggct
      121 acctgtacct cgatgatatt accgggcttg cacaatacag gatcagacgg agacgaaatg
      181 ggaagtgcga tatctattgc cggatgagca aacagttnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn atgcgtttga gttgatgctg aacggccccg ccacgacctc gttgccttgt
      361 ctcagttgaa gttgatctgt aaaactgtaa ttcgtaaaaa cctgcgccga ctgaccataa
      421 caagccggct gcggagtttg aaaagtaacc caggcatagt ccgcagtagt gggttggtaa
      481 ccgacataca acgaccgctc cacttctacc gcctgatcac cccgaatggc ccgcagtttt
      541 acgatgtagg ttgcatcaga caaccacggg cctgtgaaag tttgcggagt ggcagaaagc
      601 gacaacggaa catcgttcaa aaaccactcc agtgagtcag ggacaccgag tattcgcggc
      661 gtaaccgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
      721 aagaccaata ccgccaccat tttctgtttg agccaaaagc gtattgacaa cgccatcgtt
      781 gttttgaaac ccggccatgt agccaacatg ctcattcagg atggaaatat cattaaatcc
      841 accaaaatcg ccggaagcca ggtttggcgt tacttgttga taaaaccatg tctggccccc
      901 gtcgttggta atgatggcac ggttgcctac aaaggagaat ccgttttggc tgtcaataaa
      961 ttgaaattcg acagtcccgg caggcaaagg ttcactcacc ggcagccaat tcgccccgcc
     1021 gtcggtggtt ttaaaaaaac gggaatccgc gagcgcaaaa cccagatctg catttaaaaa
     1081 ttgaatcctg ttgaactcaa ctggtgtgga ctgaacttgc catgtgcctg ccccatccgt
     1141 agttttcatc aacgctcctt caatggacag cacccatccc aactgtggcg acacaaaatc
     1201 cacatccgtt agtcttgtat tgctcggcgt tccaagtggc agccagttgg ttcccccatc
     1261 ggttgtttta aatatgcgac cccagtcgcc caccacatat ccgttttgat ggtccaaaaa
     1321 atctaaagac caacactggc cgatgttgaa accaaattca tacgtgtacg gcgaccagga
     1381 acttcctcca tctgtagttc gcaaaatgag gtagtcggcg ccgaaaaagt catagcctac
     1441 cgcaaaacct ctttgctcgt tggtaaaaaa taagtcgttg aagatataaa cttccaagcc
     1501 ggtgagttct ccctgttcat aagccttcac ccatgattgg ccggcatcca ttgttttcca
     1561 tatatttcca ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnncgctt cccgcattgg gcacaacagg cagtatttcc cattgggcat gcccaaccat
     1681 agcactactc aacaaaatca aaaaaagtat aaaatgtcgt ttcataaggt ctgaatagag
     1741 gtagaataat gacaagcgat ttttaaacga taaaacaggc tttttagatt tccgaggcag
     1801 ggtcgaatat catgttgggt aacagcaaca aaagtagtgc aaattccgta ttactcgccg
     1861 aaaaccgcag ccggctattg gcagtttcgc agggaagcag ttacatttgc acctgagtct
     1921 tagaaacaac acaaggacgg cctgacggaa tcacctcaaa cacctttgag tatgcgattt
     1981 tattttggcc ttgcgcaaag ctgcgcgctc ttgttctctc tctccgcctt cattgctacg
     2041 gcacaaacca cccaatgcgg catcactgta aatgccggtt ccgatcaggt gttttgcacg
     2101 cccggtcaaa tgctgacgct gaacggcgcc gttaccggcg atttcctcag tgcagcctgg
     2161 tcgccgacta ccggcattgc caacgccaac agcctcaata ccaccctcac ggccaatgct
     2221 gcgggcaact acaccctcac ggcgcgtacc gtgagcggcc ccaacctggt agtcaacggc
     2281 gatttcagtc agggcaatac cggatttacc acgcaataca ccctgggatc gagcgggcaa
     2341 ttcggaccgc tcaccaatac cggcacttac ggcatcacta ccgtagccaa tttgctgcac
     2401 agccagtttg cgccttgctc cgaccataca ggcggaggcc agatgctggt ggtcaacggc
     2461 gccaccacgc cccgtaatat gtggtgccaa accgtgaccg tgcaggccaa taccgactac
     2521 gcgttcagcg cctggtttgc cgttgtggct acacaaaacc cctccctgat gcgcttcacc
     2581 atcaacggca acaccatcgg caacaacttc acgctgccca atgtgacctg cgaatggacg
     2641 cgctttttca gggtctggaa cagcggcgcg gctacctccg cccagatttg tattgccaac
     2701 gtgagtactt cagcagccgg caatgacttc tgcctggacg acatctccct gcgcaccgtc
     2761 tgcgatgtgg ccgacgtgat cgccattgcg cccgccaatc tgaacgcagg tttccaaacg
     2821 aatccggttt gcgccaacag cgcggctttc gatctcaata ccctgctcag ccccggcagc
     2881 accgccggcg gccaatggac gctcaacggc gctccggcta cgacattcca acccgcagta
     2941 ctcggcgccg gaacctacat gctcaactac agcgtacagc aaggcagttg ctcccgcgac
     3001 agcacccgca gcatcaccgt ccatccgctg cccgacgccg gaactgccgc acagccggcc
     3061 caggtatgcc ggggcacagt acaaaccctg atgctcagcc agttgctcat cggagccaat
     3121 gccggcggaa actgggcgga gacctctccc ctaccctcgc cacccggcgc tttcaatgcg
     3181 ggcgcaggct cattgcagac gcaaagcctc acgacgggac aatacacgtt tcgctacatc
     3241 gcagccggca cagcgccttg tcccaatgat accactaccg tcagcgtcgt catcacagcg
     3301 ccgccggttg cacaggccgg ccccgatcag acattggatt gcgtcatgcg gcaggtttcc
     3361 atcgggagtt cggcttccag ttccgggcca ggcatcgttt acaattggtc atcgcccacc
     3421 ggaggcagcg tggcacaacc gacagccgcc attacggaca cggaaactgc aggcactttt
     3481 gttctggcgg tcaccgacaa caacaccggc tgctcggccc gcgatacggt gatggttgcc
     3541 agtctgatta ccaaccctgt tttcagtctt gacattcagc cggttacctg ccatggcgac
     3601 aacgacggca gcatttccgt gagcaatgtg agcggcgcgg ccgaccccgc catgtttcaa
     3661 ctcaatggtg gtaccgcgca aacgacggga atttttaata acctcccctc cggtaactac
     3721 accctgcgcc tcaccgatgc caacggttgc tttgcagaac gccaagccat actgcctgaa
     3781 ccgccgccca tcatgctcga actcggcagc gacctcagcg gcacgccggc cgtattgcca
     3841 ttgggcgaca gcaccaccct ccgcatactc accaatctgg cgcccgatca gatacaaagc
     3901 gcgatttggc ttcccatcat cccggattgc agcaactgtc tgaccgctac cgtcaggccg
     3961 gtggaaaaaa ccacctacat cgtcatcctg accgatgcca ataactgcgt ggattccgcc
     4021 tcactcacca ttgttattga tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa
     4201 cgcaagaacg tcctgcccaa cgaccctgac tccggctgga acgggcgcat caaagacaaa
     4261 gaagcggcac agggagtgta tgtgtatctg gctgaactgg aattgataga tggtacgagt
     4321 atagttgttt ccggggagac gacgcttata agatagacat tacgagccgg ggagaatgcg
     4381 tttgaagtct tcgtaagtca aacactcaat tttggggaat tgaatacgag aaagaacccg
     4441 aaaatgcctg tcatcggaaa ctaaatagtc tgcattggca gaaaccatac aattgatgaa
     4501 tttatcgtca tcagcatcag gcgcaataag cctgaagtca aagtagatgt ttactttttc
     4561 aacatttgga agcatgagca gcacagacat tacaagttct gctgtctctt ggtcataaat
     4621 ttgggatagt ttttcttcgt actccaacaa aatttcagtt gaaagacaca gcatgaactc
     4681 ttgcttcaac aaggcatcga aaacaaagga ggccagagat ttccccgata tagaccgcac
     4741 tataatattg gtgtctatga caacctttaa cctatccgcc atttctcttc agtaattcat
     4801 gaagaagttc atccgtgtat tttttttcgt cccaaagctg gtcggctttg gcccgggctt
     4861 tggcaaaaag gaaacgggca ataagtattt ttaaatcttc caaatactga tccggcactc
     4921 ctgctgagta cattttcaat agttcaattt gaagatttga taaaggtgca gtacgttttt
     4981 cgacgctcat ctttaagata ttttacagcc aaagttaaac aaaggaacaa cgatatatga
     5041 ttcggttatg tttctttaca aaaaatctca tcaatcgccg catgaatttc ctggcccatc
     5101 tgctcctcac ccacgacgac gaaagcctgc tcatcggcaa tttcatcgga gatcatgtgc
     5161 gcaaccacga actgccccag tacccggtcg gagtacaaca aggcgtctgg ctgcatcgcc
     5221 ggatagatac gtttaccgac aatcaccctg cggtgaggga gtgcctgaaa ctgttgcgcg
     5281 catcgcatgg aaaatacgcc ggggtggtat gggacgtgct gtcggatgca ctgctttcgc
     5341 acaattggga ccggtttcat gatgcctctt tagaggtgtt tactgcccga atgtatgaag
     5401 ttttggagcg caacctacac catattccgg aggcgttgcg aaaaagagta ccgctgatga
     5461 ttgcagacaa ttggctgatg aaatacgccc gagaagaagg cattgagttc actttcagca
     5521 gattacaact gagagcttca caaccggaat ggttaaacgg aacgaccgaa agcctgcgcc
     5581 tgcactacgc cgcattggag gaaggattct tgcagttctt tcccgaactg atgacacaca
     5641 tcgctaccct tcgaagtgaa atgaaagcgt gaagccgcgc gagaggagtt tttccaatac
     5701 cgttgattcc tgcaggctac ggttgtacag cagcacattg ccgatgccgt gcgaaaattt
     5761 gatctccggc gaaaagataa agtagggcaa atagaattga ataccggccc catattccaa
     5821 tgaaaaatcg gtgggcgcta tgcgaactaa gttttccgaa ttgcgcgcac gcgaatcgct
     5881 ggccacgtcg aaggcatact ttaccccggc caccacgaag agacgcatat cccggtaagg
     5941 cgctgattta taacgcaaat gaaaaggcgt ttccaccaat accgactcta cgcggcgttg
     6001 ggtgaaagta gcctgacgcc ctcggcgatc gtaagtgatg ttgcgttctg cgaaagaaag
     6061 cgtgggcatg aagcgaaaat cgaaatgctc gccgattttg aggttggtga tgatgcccag
     6121 attgaagccc ggcccggtag ccgactctac ccgcgtgatg ctgtcgcttt caagaaagcc
     6181 cgccgatcga aacggcttgt agctggagtt gttgatagcc agagtaatac cgaaatagta
     6241 aggtttctga ttgaaatcca ggtaattgta attaccgccg ccgctgtact gagcttgcag
     6301 ccccgtgacg gccaatgcca gcaggaatgc cggaagaact atttttgacc cgaataaatg
     6361 gaacaaatgc ccagtgtgag aggtatgcat cggttgttgt tgtatcctgt tttttgtaac
     6421 acctccaaaa acgcgtcgcc atcgggaaaa gcctgtaccg actcgtacag gtagcggtag
     6481 gctttggggt ctttggaagt cattttgccg ataaaaggca gaatatttct gaaataaaaa
     6541 tgaaacaatt gcttgaacgg aaacgcaacg ggctttgaaa actccagaat tactacctgc
     6601 cctcccggcc tgagcacgcg ccgcatttcg gcgaggccca gttccaaatg ctcaaaattg
     6661 cgaaccccaa aagcaacggt cacagcatcg aacgtattgt ctgcgaaggg aagattctcc
     6721 gaatcggctt cctccaaacg gatgacctcg tccaagcctt gtttgctgat tttctgtcgg
     6781 ccaatggcca gcatatctgc cgaaatgtcc acgccaatga tagaatcggg cgagatgcgg
     6841 cgggctgttt cgagcgccac atcggccgtg ccggtagcca cgtccaagat gtgtttgggc
     6901 tgtttgcttt cgagcagctt gatggctttt ttgcgccagt tgcggtcaat gccgaggctg
     6961 agcacccggt tgaggaggtc gtaatatggc gctatccggt tgaacatggc cgatacctgg
     7021 ctcttcttgc tgccctcgct gctgtatggt tttacttcca ttttgttttg tttttccggc
     7081 caagaccggg cacggcgtca accgcgcggc aaaagtaagt cggaaaccgg gatttttgtt
     7141 cacgaataaa aatcatgcgc gtttagccag ccacatcatc cccgcgtaag acaagccgca
     7201 cagccccgcc aacaaaaccc acaaagcgcc atacccaaag tgatcggcca cgttcatgcc
     7261 caccaaaggc gcggccacat gcgccaccga atacgacaaa gagtacacgg ccatgtattg
     7321 cccccggttg aaatcgttgg agcgattcat cgaaaaagaa ctggcaaaag gcatggccag
     7381 catctcgccc agagtgatga caatgaccgc tacgacggcc aacatgaccc agtgtgtggg
     7441 cgccaaaaac aataagtacg aaacggccac agccacaatg ccccaacgga tcagccccag
     7501 atgcttataa cgcccctcga ggctatatac aaagggcatt tcaaagagcg caatgagcag
     7561 gccgttgagc gccagcaacg cgccgatttg gccctcgttg agcaaaaaat gctctttaaa
     7621 atatactggt atcaccgaaa aaaactgcat gaaggccacg gcccacagcg tgatcatggc
     7681 agcaaaaacg aggtagggca catcgcggta aggggatgcg accaaagcag ggcgttcgac
     7741 gacgggtttg ctgcccggcg tgcccgctgc tttgggtttc aacataaatc gaaacagcag
     7801 cgcggcaccg atgcaagtga tgccgtcggc caaaaaaagc caattgtacc ccaggctgtg
     7861 cgccagaaag ccaccgagtg cagggccgat ggaaaaaccc agattgaccg ccagtcgcag
     7921 cagtgaaatg gagcgcgtgc gggtttcggg tacgctgtaa gccgcgacag aactcgtatt
     7981 ggccggcctg aacgcctcgc cgatgatgct caaaaagaaa atggtgacgc tgatggccca
     8041 aaagctgcgc ataaacatga gcaacaggaa cataaatccc gtggagaaca gcgaccaaaa
     8101 catgaccgga tagtaaccgt ggcggtcgga aagcttgccg ccggccatgg tgcccaagat
     8161 ggaacctacc ccaaaaaagc tcatgaccag gccggcctgc catttggtga actgcaattc
     8221 ctgagtgaga taaacggtca taaaaggtat gaccatggtg cccgcccggt tgatgaaagt
     8281 caccaacgac agcagccaca tatcgcgtgt cagtccgtga aaggagttgc ggtacaagtg
     8341 tacagtgcgc gtcagcatag gcagtcgggt gtaaaaagtg ttcagtgcaa cgcagtgctg
     8401 ccgacaatag tttctgaacg aggcatctaa ttggcagtca aaccatacaa aatatggcag
     8461 agcagtcata gcctcgcttc aggcagtaat tttatacctt tgcaggcagg acgaaaatca
     8521 gccacgcggg acgcagccat ggctacaaaa aatcaaaatt tccatcatga attacctcct
     8581 tatcggcgcg ggcagttgta tcggcggcat gttgcgattc gggctgtctc aatggataca
     8641 gaccagaacc gaggcagttt ttccttatgg cacgctgtgt atcaacctgt tggggtgttt
     8701 tttgattggc ttggtgtttg gctggagcga gcgcacggct atttctcagg aatggcggct
     8761 gttgctgact accgggctgt tgggcggctt caccacgttt tctgcttttt ccaatgagac
     8821 ggtgggcatg ttgcgctccg gttggtatgc cggggcggta ctgtatgtgt tgggcagcgt
     8881 gttgccgggt atagcggcta ctttcggcgg gtattgggtt gcgaaggcgg ggggagggga
     8941 ttttaaatat cttcaattgt tattgggtgt caattccaag gtattattgg aggtaatagc
     9001 ataaatgcca gtattatagt tgcaatagtc aaaaaaagag caattagann nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nagaattctt tattcgttat gcctattttt tttattatcc
     9121 cccaatagaa aagcaatacc gtaatgatcc aatgatataa tgaaagtatc atataaggca
     9181 ccaacatttt tttttcaaat aaaaaaactg ttgaatgggg gtcactcaat tctaataccc
     9241 aatccctcat tgaagcccaa ctcaagtaga ttaatccagg taagaaaatc aagtaataaa
     9301 ctatattttc aaaaattttc ccaaaattag atttcatagt ttgttggatt aattgtcgtc
     9361 tttgaaaaaa atctttgtaa gccttttttt aaagtaatgt acttaatcca tgccgacagg
     9421 aatgctcgaa aatgccctta gtaaaataat cagaatcagc gcaatggcaa taagccaaag
     9481 tacaaagctc catacaaata cactcattgt gacaccatgt ttttttctaa acccccagta
     9541 cagaagcaaa aaagtactcg ataaaaagta taaagaaaag aagatgctga aatacaaatc
     9601 tttcttagga aaattgcttg ccctgaatgc actccacaac ccaccttcgt atggattgag
     9661 catcaaccat gatacaatga tcattgtgga tagaaatagt ccgaagtaga taatattggt
     9721 caaccagcgg cttatcatat tttccattgt tgatcgattg ttttatcaga acagccccct
     9781 actccaccaa atttatcaga acccgataat catcatacct cgcgtcatcg cttttcattt
     9841 ccagcaaatc aagcaattgc cccgagtatg tttcctccaa ttccgagcgc gtttgttggc
     9901 atcgcacctc aatatcgccc tgcaacagtt ggttcagtcg ccagcggtag gtggcctgca
     9961 ttttgcgcag gatgcgttca ttggcctcta tgtgatcgct gccgggccgc acgggcgtga
    10021 tcttttcaaa agcggcattg ttgcgggcca gcagccgggc tttttccatg ataaaaaagg
    10081 cggaatgcgc ccaaccctga ctgcctgtga ccaacctggc gtaaagcacc aattgcaagt
    10141 cggcttcatt gcgaatgctg tcttcccggt agccgatgcc ccgccatttg aggtccaata
    10201 cggccagttc gtcgccgcgc tgcaacacca aatcggcccg accgtgcagc gccgtgcctt
    10261 caaactcgcc ctccacgggt tgttcggtag ccaatacctg ccagttgttt tcccgcagca
    10321 gatgcagcag gctccaggcc gattgtttca cgccattcag gaaactgatg cgctccggtt
    10381 cgcgcccgta cagcagcagc acagcgccct cgcgagccag caaagattcc gattnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn ngaggatttc accaatttga tcttgtgccg gaacacccat tgatacggat
    10621 aatacagcag ggtttccaag ctggtgaagg tttctttttc gcgggcctcc acgcggcggt
    10681 gcggcaagat gtgcaaaaac ggtcgggggc gccccaggta acgccgtggc agagccgttt
    10741 tttgtggcaa taccggccct gttttccagg catcgccggc ggtatcggca tccacatcca
    10801 acgtgatgcg gtctattcct gaaaaggccg ccgacagatc gccaaacaaa ggatgcggcg
    10861 gcgcttcttc tccctccgaa ctgtgcggca acagcaaaag cagtcttttt cgggcggcca
    10921 tcaccggacg gcgtcgttgc cacaacaggc gttcgttttg aagcgccgga ccttcgactt
    10981 ccagacctcg ggcttgcatc caggccaatt cagcgcggta ccagcgcgag aagaaatggt
    11041 cgggttcttt ttgaataaaa ttccaccaga gcagttcttc cacaggctcg atgagcgcgc
    11101 cgggacgcgt gacaaaaggc aagtggccct gttcgcgcgg gctgagttgc agcggcgagg
    11161 attgatatac cgtgcgcacc acgcgttcga gttccagata gccgagttgt gtttcgggca
    11221 gcgattccag cagttctaca atacggcggg cctgttcgcg cagggcgggc agcgtcgaat
    11281 tggacagcat gttttcatcc atactctctt tggcccactg ttcgatgtat cggaatatct
    11341 ccacggcatc ggctttggga gcgctgcggg aggaatcgta acgtttgcgc tcaaaccaaa
    11401 aacggtattg tttgcgcgct ttggccgagt cggggccgcc attttgcgcc gccaattgct
    11461 cggcttcgtc gaagtaccgg gccaccgcta tgcgccagcc ctcgccatgc aggccgggcg
    11521 tttgggccag gctttctgca atggccgccg ccagtccgtc ttctaccggt ttcactgcca
    11581 gcgacacaaa ttccatgagt ttgaaggggt ccacgggatt ccacaaaaat ccggcagcca
    11641 atttcagcac ctgcaaagtg gggcgcgcct gcgaggcaga gagcaaaccg aggctgggca
    11701 atccctcctg aatgagcgca ttgtccaagc tccggctgcg gtcggaaata tagcacagcg
    11761 gccggaaatc cgggtttctg cgcaggagcg ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn cgccgggtgc gcgccaacag cagcgagccg tcgccgcgga gttcggtttt
    11881 tccggtgcct tgtccggcca aaacccgctg aaaacgagcc agatcggtat ctgcgtccga
    11941 gggctgtatt tgcggaagcc aggtgagttg cacgcctgtt ttttcgaggg cggccagcag
    12001 tcggcgaaag tgtggcggca gtaattccgg cggttctact attttgattt cctgcaccgg
    12061 atggcgacgt gtagaaagca ccgacagcac cgcgctgaag cgatctgccc agcccggcga
    12121 aaaggctttg ccctcctcca ccccgatccg caaagcagtt tccaactcgg ccagcacccg
    12181 tactcgcggc ggttcatcgc ccgaaagggc aaaatcccag cccgccaaaa gcagttcgtc
    12241 gcggtaggcc aacagggcgg tagcggttgc caaaggatcg gcggcgaagg attcggcaaa
    12301 aaaagcatcg ggatgctcgc tgaggtgagc gctgagcaac tgccggtact gctcgatgcg
    12361 caaaaactcg tctaacggct gcgcgccggc taatcccagg tgcgattcca gcacattgag
    12421 caagccctgc ggccccaccc aaaacatacc gctccagggc ggcgcaggca tacgccaggg
    12481 ttcttcttcc aaagccagac cgaaatagag gagcatgttg tacgggtatt tggcaggcaa
    12541 acgtacaaaa gattattata cctttgtccg aaatttttat gctcgaaacc atacaacatt
    12601 gggaccaaca agtattcgaa ctgctcaacg ggcagtggca atcgccgttg ttggactggc
    12661 tggccccgct gtggcgcgac aaacgtttct ggattccgtt gtacctgctg ctttccattt
    12721 ttgtggttcg cacttaccga aaacgaggcc tgtattttct actggcactg ggactgaccg
    12781 ccggcatcag cgacgtggcg agcagccgca tagtgaagcc ctccgttcaa cggctccggc
    12841 cctgcaacaa ccccgaattg cgcgacgacg ttcacctgct cgtccactgc ggatcgggtt
    12901 atagtttcac atcttcccat gcggccaatc attttgcagc ggctgccttt ttatttttca
    12961 cgctgggcag gctgcatcgg cgtggtcgcg ggctgtggtg gctgtgggcg gcttctattg
    13021 cctacgcaca ggtatatgtg ggcgttcatt atccggcaga tattttggcg ggagccgtct
    13081 tgggcgccgc tattggatat ttgagcagca ggctatacct tcgcctcccg aatttgagca
    13141 tagcctcgat gaacacaaat cagcgcgcat gagcataagc caatacctgc ttcttttttt
    13201 tgccgtcatt ttgggcggag tcatcggttt tggacttcaa aaatacgagc gcaaccacct
    13261 caagatcgtg ctggctttca gcggcgcttt tttgctgggc atttccgttt tgcacctgat
    13321 gccggcggtg tatgtgcagc aaaaagagca ggcaggctta tggattttgc tcggtttttt
    13381 catacaactg gcattggagc agctttctac cggcgtagag cacggccatg tacacgccca
    13441 ccctcatgcg ccgcgcgggt ttgccattca ggtgatgatc ggcttgtgcc tgcacgcatt
    13501 tctggaaggt atgcccttga gcggttatga acaggtgcat accgcgcacg atcattccgg
    13561 tcatgcagag catcacttat tatatggtgt agtgttgcac aaagctccgg cggcttttgc
    13621 gctggtgatg ttgctgctca gttcgcaatt caaaaaagcg gctgtgattt gctgtttgtt
    13681 ggtatttgcc tccatgtcgc cgctgggcgc tttgctcact caatttatcg gagttaatca
    13741 aggcactttt tccattttgc tggcagtggt tatcggctcg ttcctgcaca tagcgaccac
    13801 cattttgttt gaatccggcg aaagcaaaca ccaccgggtc tcctggggga aattggctgc
    13861 cattgtggta ggcgtagcac tatccatgtt gaccatgctt tgaccccggc gctacgaaag
    13921 gacttcatta aaaaaaatgc cgatttctac gcaagttgta aaaaagaggt attttagcgc
    13981 gttgaacaaa acaaaataac catcagcccg atttggccta tgaaatgttg ttacacttcg
    14041 ttgattctca tattttttat atcaattgcc ggcttttcag ccaatgctca ggctaccctg
    14101 cgcggagatg tgacagacgc aggcaattcc gatccgctca tcggcgccag cgtagtcatt
    14161 aaaggtacga cagaaggcac cgtgacggaa tacgacggca cttttgaact aaaaacaaag
    14221 cagcccctgc cgctcacgct catcatttct tatgtcggct acaatgaaat agagttgccc
    14281 gtaaacgctg ccaatcaacc gctgaaaatc aagctcaatg ccagcgccat taccaccgaa
    14341 gtggtagaaa tcaaaggcca acgcatctcc gagaagcaaa aagccaaccc gctcacggta
    14401 gaatccctcg acctcatcgc catcaaacag gccgccaccg acaacttcta cgacggactc
    14461 ggctcgctca aaggcgtgga cctcaccgcc gccagcctcg gtttcaaaat catcaacacc
    14521 cgcggcttca acagcaccag cccggtgcgt agcctccaga tcattgacgg cgtggacaat
    14581 caggcgccgg gcctcaactt ctcgctcggc aacttccttg gctcctctga attggacgtc
    14641 gtcaaagtgg atctcatcgt gggcgccagt tcggcatttt acggccccaa tgctttcaac
    14701 ggcgttatca gtatggaaac caaaaacccc ttcctgcaca agggcctttc ctcgtccgtc
    14761 aaagcgggcg aacggagcct cttggaggga gctttccgct gggccgatgc gctcaaaaat
    14821 aaaaacggcc acgacgcttt tgcctacaag gtcaacttct cctatatgcg cgcttatgac
    14881 tgggaagcag acaattacga ccccgtggac ggcaccgata ccggcgtgga caatcccggc
    14941 cgctacgatg ccgtgaatgt ctatggcgat gagtacttta caggtttcga ctattccact
    15001 ttcgacctgc acacttaccc cggcctcggc gcctggcacc gcaccggcta ccgcgaaaaa
    15061 gaccttgtgg attacaacac ccgcaatatc aaggccaatg ccgctttcca cctgcgcacc
    15121 aatcccgctg ccggagacgc ctcgcccgaa ctgatttatt cttccaattt tggcacaggc
    15181 acaacggtat atcagggcga caaccgcttc agcttgcgca atatccgctt tttccaaaac
    15241 aggctcgaat tcagaaaaag agatcagtat ttcattcgcg cctatatgtc gaaggaagac
    15301 gccggcgatt cttacgaccc ttattttaca gcacttttgc tgcaagacag cgccaaacgc
    15361 aacacagatt ggggcataga ttatgtggac tactggctgc gcaatgtggc gcccgtcatc
    15421 aaaaacgcag agggttttcc gcgcctcaac attgtctttg atcccaatac cggacaggtg
    15481 accgccacct tcgaccgcga ggcggcattg gcctggctgg ccaacaacaa agaccgaatt
    15541 gacgcctggc acacacaagc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgcttca tagatcgccc ggcgctgtat
    15721 catgtacacg gcgagtataa attcaagcct tcttttctgg aatatctgac cgtgggcggc
    15781 aatgcccggc tttatacgcc cgtatcggaa ggcacggttt tttatgatac ggccggcata
    15841 cgactgacca attttgagtt cgggatgtac agcggtttgg aaaaagccct gttcgacaac
    15901 cgcctgaaag cctcgctggc act
//
LOCUS       DAORLF010000043        15870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1985_length_15870_cov_2.253746, whole genome shotgun sequence.
ACCESSION   DAORLF010000043 DAORLF010000000
VERSION     DAORLF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15870
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_15870_cov_2.253746"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..>1768
                     /locus_tag="PK198_05900"
     CDS             203..>1768
                     /locus_tag="PK198_05900"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38297.1"
                     /translation="MKKRIFTNLLFVFFLTNLGANPIIIQETLVWQSTPESFDYWGNP
                     CERLRFNGCAFGEEQPDMPWFFKRFEVSGPGELTVQLMDARYEPFDKTMCPGNELVSE
                     QLVFQTSVTTDRGRHTGSVSFIPVIRRGGRWERLVSFTLQVEHRPSNMVEFRDPAGTD
                     RSALANGDIYKIAVTQDGVHRISQSFLRSLGINTDNLDPRKISIYGNGGGMLPQFAGA
                     DRSDDLVENHIIVSGEEDGRFDANDFILFYAQGPDRWVYNAAENKFDLEKNVFDNKNY
                     YFIKIGTQNGARAANQASLGSGVITITTFDDYARFEEDRKNLLHEWQGKATGSGLKWF
                     GEKFKNQREKAYNRLFSMPNLTGEAVRLEAEMALRAGRSSRFEIEIGGQTFQSNSVGS
                     VILSGVNDNITEYARIARLQVNPVLSGGDINLTLRYPYPGGAADESEGWLDWIQINAR
                     RALTMSSSQMHFRSLAALTQASATYQIDGASSRIRVWDISDPLRPKVQAANLNGAQLS
                     FSAAGQALRQFIAF"
     assembly_gap    1769..1816
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1817..4072
                     /gene="porU"
                     /locus_tag="PK198_05905"
     CDS             <1817..4072
                     /gene="porU"
                     /locus_tag="PK198_05905"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRF38298.1"
                     /translation="NQNYHGLDHIDMVILYHPDFTAEAQQLAQHRITYNDFSVALVRI
                     DSLYNEFSSGRQDPTAIRDFAKMLYERNPRFRYLLLFGDASFDYNNAYGLGNHFIPTF
                     QRDGLNPLNTHPVDDYFGLLTGTNPNNILGGQMNIAVGRLTVNTAQQAAVAVRKIMHY
                     DNNSNTFGDWRNRLVFVGDDEDGNTHTRDANQVADIIEGLSPSFNIDKVFLDAYQQRS
                     TPAGAFYPEVNEAINRAMFRGVLALTYFGHGGPKGLAQERIMTISDILSWRNMDNMPL
                     LITATCTFGGFDDPAFVTAGEEAFLNENGGAIALFSTTRAVFANYNRELSEEALRQLI
                     NRQDGGFPTIGEALRRAKNAFTADVSVLANSRKFSLIGDPALKLKIPEYQVLTTKINT
                     QDVSSTSSDTIRALQKVTIEGEVRDLNGQVMTQFNGAVLPTVFDKALNLSTLGQDPTS
                     FPYPFSVRRNTLFKGRATVSAGTFRFTFVAPKDIDYRFGTGKIGYYAFDPDKAVDAAG
                     NYNRIVVGGTDPNALTDNKGPNVDVFMNSDGFVFGSITNSNPVLLVHLEDENGINVSG
                     NSIGHDLEGVLDEDTQNTLLLNDFYEADLDDYTKGKVRYPLYSLKEGRYKIRVKAWDV
                     ANNPAEGYTEFVVANSAEIALQRVLNYPNPFTDFTCFQFDHNLAGQEMDVLVQIFTVS
                     GRLVKTLEERVMGDGALRLDDCIRWDGRDEYGDRLARGVYLYKVRVRSVSTGSTVLSG
                     ESGFEKLVILK"
     gene            4154..5362
                     /gene="porV"
                     /locus_tag="PK198_05910"
     CDS             4154..5362
                     /gene="porV"
                     /locus_tag="PK198_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRF38299.1"
                     /translation="MKYQLFTIAAILMLLIGSVQTAEAQCIKGPDGKFYNLDGSPCVN
                     TVLTAVPFLRIVADARSGAMGDVGIGLSPDPNAIHFNPSKLAFADQKTAVSATYTPWL
                     RALGLNDVYLAYLTGYHKLDDLQTLGFGLRYFSLGDIPFTDVNGEPLVTGRPNEFEVT
                     AAYARKLADKFSASVSAKFIFSNLAAGQIVSGEVIEPGVAGAADFSLTYNTPIEMKSM
                     NSDLMVGLAISNIGSKISYTKSLTKDFLPANFGLGAAWNIDINDFNRITFAADVNKLL
                     VPTPCQGPDCDTSPTDGVPDYKAQSPISAIFSSFGDAPEGFSEEIKELMYSFGVEYWY
                     DKQFAVRAGYYSEHSQKGGRKFLTAGLGLKYNVFGLNFSYLIPTTNQRNPLDNTLRFS
                     LLFDFGAFEE"
     assembly_gap    5532..5657
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5879..7318
                     /gene="miaB"
                     /locus_tag="PK198_05915"
     CDS             5879..7318
                     /gene="miaB"
                     /locus_tag="PK198_05915"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRF38300.1"
                     /translation="MLYDDNPTLVGIPKEIDENRQGEALFWEDARSGSTAGGKRFYIE
                     SYGCQMNFSDSEIVASVLAEEGFSPTRFMEESDLILINTCSIREKAEETVRKRLRMFD
                     LEKRRRPGVLVGVLGCMAERLKKKFLEEEKLVDIVVGPDAYRDLPKLIAGAEDGEKGI
                     NVFLSREETYADISPLRLDSNGVSAFISIMRGCDNMCSFCVVPYTRGRERSRDAFSIV
                     AEAAQLFEQGYREVTLLGQNVDSYKWQNPENGHVVSFAELLEMTALVHPGLRVRFSTS
                     HPKDMTDEVLHIMARHENICNYIHLPVQSGNSRILDLMNRTYDREWYLGRIEAIERIL
                     PDCAISSDIITGFCTETEEEHRDTLSMMERANYSMSYMFAYSERPGTPAAKKYEDDVP
                     EEVKKRRLNEVIRLQNQISLRHNLADVGKTFRVLIEGDSKRSDQEFKGRNSQNKMIVF
                     PKQPGLKPGDYVNVLVKSATGATLLGEMC"
     gene            7482..8378
                     /locus_tag="PK198_05920"
     CDS             7482..8378
                     /locus_tag="PK198_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRF38301.1"
                     /translation="METTLKNTKKAAAPIQKLSRPKKLIAFGWYGGKFSHLDWLLPHL
                     PECLHYCEPFAGSGAVLLNREPSPVETYNDIDGEVVNFFKVLREEKEALIEQIALTPF
                     SREEFAVACQLDPDLTSLERARRFYVRARQVRTGLAQTASVGRWANCKNTSRAGMSGV
                     VSRWLGGVEQLDFIAERLLRVQIENRPAIDVIKLYDSKNTLFYCDPPYIHETRGDTKS
                     YGFEMTDSDHKTLAETLNAVEGLAAVSNYECELMEELYPSGKWTKIYSTEKTIHSTKD
                     IRQEVLWVNYSTSRLEAGSGSR"
     gene            8408..9706
                     /locus_tag="PK198_05925"
     CDS             8408..9706
                     /locus_tag="PK198_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF38302.1"
                     /translation="MSELQTIKQRFGIIGQSALLDRALSTAVRVAPTDLTVLLSGESG
                     VGKEVFSRVIHSLSARKHNKFIAINCGAIPEGTINSELFGHEKGSFTGAANERKGYFE
                     AYDGGTIFLDEIGEMPLDTQSYLLRVLENGEFIRVGSSTVLKTDVRIVAATNVNLEER
                     IKKGKFREDLFYRLNTVPIKVPALRERPEDIFLLFRKFVVDFSEKYRIQPIQLDEKAR
                     RILENYAWPGNIRELKNVAEQVSVLAEERLLDAESLLRVAPMILQRHLPMIADKNGSE
                     GGGMQEREILYKLLFDMKGDLNDLKTLVFELIRSNDLNVPDTQHLRVLQSPTTYAPAE
                     PAAMHIAPEAPLGRAIVPVREESYDMSAPAVMLEHSRHHDDDYESMEVLEENLSLIEN
                     EKELIRKALRKHAGRRKDAAQDLGISERTLYRKIKEYDMQ"
     gene            9703..10203
                     /gene="lptE"
                     /locus_tag="PK198_05930"
     CDS             9703..10203
                     /gene="lptE"
                     /locus_tag="PK198_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRF38303.1"
                     /translation="MRQFIFFTSLIAVLAASGCYTLSGVSIDPNIYKTYYVEQFVNNA
                     DNAPPTLAVEASETLKEKIRTESRLVFNDTDPDIEFKGKVVSYVVTFEAPQPGEQAAI
                     NRLRITFAVEYIDHKEEKKNWKQNFQFFFDYPTTTDLASIQSQAHEAILRQVMEDIFN
                     KTFTDW"
     gene            10280..10774
                     /locus_tag="PK198_05935"
     CDS             10280..10774
                     /locus_tag="PK198_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRF38304.1"
                     /translation="MAKPSIAELESHMPQTGSVAWIGIRPGVRQPLIAVEKVEAVAGT
                     GLRGDHYDNPGGKRQLTLIQGEHLDAAASILSQSEIDPGLTRRNIVVRGINLQALKER
                     RFSIGEVVIEATGDCVPCSRMEENLGTGGYNAMCGHGGITARIVSSGHISVGDEVKLL
                     PENA"
     gene            10767..11237
                     /gene="ybaK"
                     /locus_tag="PK198_05940"
     CDS             10767..11237
                     /gene="ybaK"
                     /locus_tag="PK198_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRF38305.1"
                     /translation="MPKTNAVRLLNAAGIAYRLVEYRYDEAQLGVDKIARDNGLELER
                     IFKTLVLKTETGQVVVAVTPGDKALDFKTVARLAACKKIHLAEMKELQSLTGYIRGGC
                     SPVGMKKNFPVFIDQSALEHERILVNAGARGLLMEVAPGELGSLCHAVFEYITA"
     gene            11250..12122
                     /locus_tag="PK198_05945"
     CDS             11250..12122
                     /locus_tag="PK198_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF38306.1"
                     /translation="METAELLRKVRKLEIKTKRLSRHLFAGEYHSAFKGRGMSFSEVR
                     SYQFGDDVRHIDWNVSARTGQPFVKVFEEERELTVMLLVDMSASSVFGAVAQSKREFQ
                     IEIAALLAFAAIQNNDKAGIIFFTDEVELFIPPQKGRHHILRIIRELVNFEPRRKGTN
                     IAAALEFFSHVIKKRSIGFLISDFVAPDYEHALRIAARRHELIGIQVGDPLERQLPDA
                     GLLHFTDPETGRTAWMDSSSAELRREYAAAAQARDDYFRNAFAKGGADALQIGTDESY
                     LHALMGFFQRRRIP"
     gene            complement(12172..13743)
                     /locus_tag="PK198_05950"
     CDS             complement(12172..13743)
                     /locus_tag="PK198_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF38307.1"
                     /translation="MAGAQKPPLDWFHLDPAKDGFPGLRTNKAYEGPLKGKTSQTVIV
                     AVIDSGVDAEHEDLRNVMWTNPGEIAGNGKDDDDNGYIDDIHGWNFLGGKDGRNVGAD
                     NLEVVRQYRAYDARFKNADASKLSAADKKDYEKYKEYEGVIAEKRKQAKQNMELYGPV
                     VEALNDLVKHIGKDPFQVSEEDVRKVKSNKPSVTRATRVALSVMGGSGQTLGDVKRDL
                     EEYYTHLDDEYNYNYNLDYNPRNIVGDNYADVDEKGYGNNDVKGPDATHGTHVAGIIG
                     AQRNNNIGMDGVAGNVRIMSVRTVPNGDEHDKDVANAILYAVDNGALVINMSFGKGAS
                     PHKAAIDRAVRYAAKRDVLLIHGAGNDGQEIDLQNNFPNRSYLKKGLFGPKYAANWME
                     VGATHWKGGEMLAAEFSNYSSKYVDIFAPGVDIYSTDVDNQYRNLQGTSMAAPMVAGA
                     AAVLRSYFPQLTAVQVKNILVSSSSKQNIDVIIPGGEDKVPFKSLSTTGGILDLEAAV
                     RMAEQTQGKRKTVAP"
     assembly_gap    13754..13763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13923..14783
                     /locus_tag="PK198_05955"
     CDS             13923..14783
                     /locus_tag="PK198_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF38308.1"
                     /translation="MKLLKILILLLLPAFAHAQYWEAGAFAGLSNYLGDLSANSQTIY
                     MQNTGFSGAVFARYNINDLTGVRLALGYAGLSGTDALADSEAIRTRNLSFSSDVIELA
                     LTGELNLPGYQPYNLNRPLSPYLFAGISAFYHNPTANLGGANIRLRPLGTEGQGLQDR
                     PAPYSSIAFAIPMGLGVKYAVTDRLNIGIEFGARMTFTDYLDDVSGTYLSFPELLAGN
                     GPLAAALGNRSGELLGGEPVIVPTGTPRGDNRARDWFFNAGLSISYNFLDSGLVGSRN
                     RIRKKAGCRT"
     gene            14942..>15142
                     /locus_tag="PK198_05960"
     CDS             14942..>15142
                     /locus_tag="PK198_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38309.1"
                     /translation="MSNSTIRYSFLLGMIALAVVSRILPHPHNFTPIGAVALFGAAYF
                     TRQWVAMLAPFIALWLSDLFINN"
     assembly_gap    15143..15191
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15192..15499
                     /locus_tag="PK198_05965"
     CDS             <15192..15499
                     /locus_tag="PK198_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38310.1"
                     /translation="LWVYGAFALINLLGMAALRRVSAARVGGSAWAASVLFFIVTNFG
                     VWASGGMYPKNTEGLIACFVAGLPFFGNTIMGDLFYSLVLFGAFEWAQARKLIPAAA"
     gene            15523..15714
                     /locus_tag="PK198_05970"
     CDS             15523..15714
                     /locus_tag="PK198_05970"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRF38311.1"
                     /translation="MPEKPSRTNNPNPAPLREGIDYYIENGRYVFTEHFLRQRGYCCQ
                     SGCRHCPYGFQKEIIPAEE"
BASE COUNT         3751 a         4265 c         4065 g         3556 t
ORIGIN      
        1 tggtcctgaa gcgcaacgat ggccatacaa atgatgccga gcgtaaggaa tggtagtttt
       61 atcatagtca gaatcgggct ttaaaacgtt aaacaggaca acagccgaca atgttaagta
      121 gcaatacctc cgaaatgcca aacgttatcg ggcggtaatt tttgccgact ttaacaatcg
      181 cgccgtaaaa tataacaagg tcatgaaaaa acggattttt acaaacttgc tctttgtatt
      241 ttttctgaca aacctgggag ccaaccccat cattattcag gaaacattag tgtggcagtc
      301 cactcccgaa tccttcgatt attggggcaa cccctgcgaa cggctccggt tcaacggctg
      361 cgctttcggc gaagagcagc ccgacatgcc ctggtttttc aagcgctttg aggtgtccgg
      421 gccgggagaa ctgaccgtgc aattgatgga tgccagatat gagcctttcg acaaaacaat
      481 gtgtcccggc aatgagcttg tttccgaaca acttgttttt cagacatccg tcaccaccga
      541 ccgaggaagg cataccggct cggtcagctt cattcccgtg ataagacgcg gcggcaggtg
      601 ggagcggttg gtgtctttca cgctgcaagt agagcatcgc ccctccaata tggttgagtt
      661 cagagacccg gccggaacag atcgctccgc cctggccaat ggcgatattt ataaaattgc
      721 agtcactcag gacggggtgc atcgcatttc tcagtccttt ttgagaagtc tgggcatcaa
      781 taccgacaac ctcgatcctc gcaaaatcag catttacggc aatggcggag ggatgttgcc
      841 gcaatttgcg ggcgccgacc gctccgacga cttagtggaa aatcacatca tagtgtcggg
      901 cgaggaagac gggcgttttg atgccaatga cttcatcctg ttttatgctc aaggtcccga
      961 caggtgggta tataatgctg cggaaaacaa gttcgacctg gagaagaatg tgtttgacaa
     1021 caagaactac tattttatca aaataggcac acaaaacggc gccagagcag ccaatcaggc
     1081 ttcgctcggt tcgggagtta ttactattac cacttttgac gattacgccc gctttgaaga
     1141 agaccgcaaa aacctgttgc acgaatggca gggcaaagcc accggctccg gtttgaaatg
     1201 gtttggcgag aagttcaaaa accagcggga gaaagcctat aaccgcttgt tctccatgcc
     1261 caatctaaca ggagaggcgg tgcgtttgga ggctgagatg gcactgaggg caggccgcag
     1321 ctccagattt gaaatagaaa tcggaggcca aacctttcaa agcaactcgg taggcagcgt
     1381 catactcagc ggcgtcaacg acaacatcac cgaatatgcg cgcatcgccc gcctgcaagt
     1441 caaccccgta ttgagcggcg gcgatatcaa cctgactctt agataccctt atcccggcgg
     1501 tgctgccgac gaaagcgagg gctggttgga ttggatacaa atcaatgccc gccgtgctct
     1561 gaccatgagc agctcacaga tgcacttccg cagtctcgca gcgcttactc aggcatcggc
     1621 aacctaccaa atagatggcg cgtcttcccg cattcgggtg tgggacatca gcgacccgct
     1681 gcgccccaaa gtgcaagccg ccaatctgaa cggcgcacaa ctcagtttca gcgctgccgg
     1741 ccaggcgctg cgccagttca tagccttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnaatc aaaactacca cggtttagac catatagata tggtgatcct
     1861 gtatcacccc gatttcactg ccgaggcgca acaattggcg caacacagaa tcacctacaa
     1921 cgacttctct gtggcactgg tgcgcataga ctctttgtac aacgagtttt cctccggtcg
     1981 gcaggatccc accgccatcc gcgattttgc caaaatgctg tacgagcgca acccgcgttt
     2041 tcgctacctg ctgttgttcg gcgatgcttc ctttgattac aataatgctt acggactggg
     2101 caatcatttt attcccacat ttcaaagaga cggcctcaat ccgctcaata cacaccccgt
     2161 agatgattat ttcgggctac tcaccggcac caatcccaac aacattttgg gcgggcaaat
     2221 gaacattgcc gtgggccgcc ttacggtgaa taccgcgcaa caagcggctg tggcggtgcg
     2281 caaaatcatg cactacgaca acaacagcaa cactttcggc gactggcgca atcgcttagt
     2341 cttcgtaggc gacgatgagg atggaaacac ccacacgcgc gacgccaatc aggtggccga
     2401 tattattgaa ggactttccc cgtcgttcaa tattgacaag gtttttttgg atgcctatca
     2461 gcagcgctct acgcctgccg gcgcgttcta tcccgaagtg aacgaggcca tcaatcgcgc
     2521 tatgtttcga ggcgtgttgg ccctcactta tttcgggcac ggcggcccca aaggcttggc
     2581 acaggagcgc attatgacca tcagcgatat cctctcctgg agaaatatgg acaacatgcc
     2641 gctgctcatt acggccactt gtacctttgg aggatttgac gacccggctt ttgtgacggc
     2701 gggcgaagaa gcctttctca atgaaaacgg gggcgccatt gcgctgtttt ctaccacccg
     2761 cgccgtgttt gccaattaca atcgggaact cagcgaagag gccctccggc aactcatcaa
     2821 ccggcaggac ggcggctttc ccaccatcgg tgaggcattg cgcagagcca aaaatgcctt
     2881 tacggccgat gtttccgtgc tggccaattc ccgcaaattt tcactgattg gggatcccgc
     2941 tttaaaatta aaaattcctg aataccaggt gcttaccacc aaaatcaata cgcaggatgt
     3001 cagctccacg tcttccgaca ccatccgggc gctccaaaaa gtgaccattg agggcgaagt
     3061 tcgggatttg aacgggcagg tgatgacgca gttcaatggc gctgttttgc ccaccgtttt
     3121 tgataaggca ctgaatctga gtacactggg ccaagacccc accagctttc catatccgtt
     3181 ttcggtgcgg cggaatacct tgttcaaagg gcgcgctacg gtgagtgcag gtacttttcg
     3241 cttcactttt gtagcgccta aagacattga ttatcgtttt ggtaccggaa aaatcggcta
     3301 ttacgccttc gaccccgaca aggcggtgga tgctgccggc aattacaacc gcattgtagt
     3361 gggaggcacc gaccccaatg cactgaccga caacaaaggc cccaatgtgg acgtgtttat
     3421 gaattccgac ggctttgttt ttggaagtat taccaactcg aatcctgttt tattggtgca
     3481 tctggaagac gaaaacggca tcaatgtatc gggcaacagc atcggccacg atttggaagg
     3541 cgtattggat gaagataccc aaaatacact gttgctcaac gatttctacg aagccgatct
     3601 cgatgattac accaaaggaa aagtacgtta tccgctgtac agcctcaaag aagggcgcta
     3661 caaaatcagg gtgaaagcct gggatgtggc caataatccg gcagagggct acacggaatt
     3721 tgtggtggcc aattcagccg agatcgcctt gcaacgcgta ctcaattatc ccaacccctt
     3781 caccgatttc acctgtttcc agtttgatca caacttggcc gggcaggaaa tggacgtatt
     3841 ggtacaaatt tttaccgtat cggggcgctt ggtcaaaacc ctggaagagc gcgtgatggg
     3901 cgacggcgct ttgcgtttgg acgactgcat ccgctgggac ggccgcgacg aatacggcga
     3961 ccggctggcg cggggcgttt atctgtacaa agtgcgggtg cgcagcgtgt ccacaggcag
     4021 cacagtactc agcggcgaga gcggatttga aaaattagtt atcctgaaat aagaacggct
     4081 tttttgcgtc tgtttgggag tggcaaggtt tttgctctat atttgcacga tttttcaaac
     4141 tcaataactt ctcatgaaat atcaactgtt taccattgcg gcgattttga tgctgctcat
     4201 cggatcggtt caaacggccg aagcacagtg tataaaaggc ccggacggga aattctacaa
     4261 ccttgacgga tctccctgtg tcaatacggt attgacggcg gtgccctttt tgcgcatcgt
     4321 ggccgacgcc cgctccggcg ctatgggcga tgtgggcatc ggtttgtcgc ccgatccgaa
     4381 tgcgattcac ttcaaccctt ccaaattggc tttcgccgat caaaaaacag cggtatccgc
     4441 cacctacact ccctggctgc gcgcactggg actgaatgac gtttaccttg cctatctgac
     4501 gggttaccac aaattggatg acctgcaaac cctcggcttc ggcttgcgct acttctcgct
     4561 gggcgatatt ccgtttaccg acgtgaacgg ggagccttta gtaacgggcc gccccaatga
     4621 atttgaggtt acggcagctt atgcgcgcaa attggccgac aagttcagcg cctcggtgag
     4681 tgccaaattc atcttttcca acctcgcagc cggccagatt gtgagcggag aggtgattga
     4741 accaggcgtg gcaggagcag ccgacttctc gctgacctac aacacgccca tcgaaatgaa
     4801 gtccatgaat tccgatttga tggtaggttt ggccatctcc aatattggtt ccaaaatatc
     4861 ttataccaag tcgcttacaa aagattttct gcctgccaac tttggtcttg gcgctgcctg
     4921 gaacatagac atcaacgact tcaaccgcat tacttttgcc gccgatgtga acaaactgct
     4981 ggtgccgacg ccctgccaag gccccgattg cgataccagc cccacggatg gcgtacctga
     5041 ttacaaagca caatcgccca tcagcgccat cttctcctct tttggcgatg cgccggaggg
     5101 ttttagtgag gagatcaaag aattgatgta ttctttcggt gtagaatact ggtacgacaa
     5161 gcagtttgct gtgcgggccg gttactactc agaacactcg cagaaaggcg ggcgcaagtt
     5221 tctcaccgca ggtttgggcc tcaagtacaa tgtctttggc ttgaacttct cttacctgat
     5281 acctactacc aatcaacgca acccattgga caacacgctg cgtttctcgc tgctgttcga
     5341 ttttggagca tttgaagagt aagaaacgga aggtactgaa acagaaaaag cgagtcggac
     5401 cggatgtttg gctcgctttt tttgtttggg caagcatatt gaatcaatca acaccatagt
     5461 aataaccatg accggagacc tcaaaatcga atgcccgaag tgcggctggg aacccgacgg
     5521 cggcgcttac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnncgc catgccgccg catttggatt ggtacaaagg cttggacgac
     5701 tggctgaaag aggagttgga ggaggtggag gaaagtgtag cggttgggtg agcaatacag
     5761 caacagccgt agagaggaag gggtagtggt ggaagaagtt ttattccatt ctgccaaatt
     5821 gtcacaaccg gaaaaagtct tacctttgct gccgattgaa cgaaaaaaga cacggcaaat
     5881 gctttacgac gacaacccga cattggtcgg tattcccaaa gaaatagatg aaaaccggca
     5941 gggagaagcc ttgttttggg aagatgcccg cagtgggagt actgccggcg gcaaacgatt
     6001 ttatatagag agctatggct gccagatgaa tttcagcgac agcgaaattg tggcctccgt
     6061 gttggccgaa gaaggctttt cgcctacccg ttttatggaa gaatccgacc tcatcctcat
     6121 caatacctgc tccatccgcg aaaaagcgga agagaccgtg cgcaaacgcc tgcgcatgtt
     6181 cgatctggaa aagcgccgtc gcccaggcgt gctggtaggc gtattgggct gcatggccga
     6241 gcgcctgaag aagaagtttc tggaagagga aaaattggtg gacatcgtcg tcggccccga
     6301 tgcctatcgc gacctgccca aactcatagc cggcgccgaa gacggcgaaa aaggcatcaa
     6361 cgtgttcctg tctcgtgaag aaacctacgc cgacatcagt ccgctgcgat tggacagcaa
     6421 cggcgtcagc gctttcatct ccatcatgcg gggctgcgac aacatgtgct ccttctgcgt
     6481 ggtaccctac acccgcgggc gcgagcgcag ccgcgacgct ttcagcattg tggccgaagc
     6541 tgcccaactc ttcgagcagg gctaccgcga ggtgacgctt ctgggccaaa atgtggactc
     6601 ctacaaatgg caaaatcccg aaaacggcca cgtcgtctcc ttcgccgaac tgctggaaat
     6661 gacggctctc gttcatcccg gcctgcgcgt gcgtttttcc acctcgcacc ccaaagacat
     6721 gaccgacgag gtgctgcaca tcatggcccg ccacgaaaat atttgcaact atatacacct
     6781 gcctgtacaa tcgggcaaca gccgcatcct tgacctgatg aaccgcacct acgaccgcga
     6841 atggtatctg ggccgcatcg aagccatcga acgcatcctg cctgattgtg ccatttcgtc
     6901 agacatcatc accgggtttt gcaccgaaac cgaagaggaa cacagggaca cgctgagcat
     6961 gatggaacgc gccaattact ccatgtcgta catgtttgcc tactccgagc gccccggcac
     7021 cccggctgcc aaaaagtatg aggacgatgt gccggaagag gtgaaaaagc gccgcctcaa
     7081 cgaggtgatc cgcctgcaaa accagatttc gctgcgccac aacctcgccg acgtgggcaa
     7141 aaccttccgg gtactcattg agggcgacag caagcgctcc gatcaggagt ttaaaggtcg
     7201 caactcacaa aacaagatga tcgtctttcc caaacaaccc ggcctgaaac ccggcgatta
     7261 tgtgaatgtg ctggtgaaaa gcgctacggg ggctacgctg ctgggggaga tgtgttgatg
     7321 gggaggttag gaaatttgga agagaataaa aggctgcaag ccattagcta caagccacaa
     7381 gcttcgagtt ttggcagatc gaagattgtg ccacacaaga gcaatatgca aggctttgtt
     7441 gtgatttctg aaaatacgca aaaaatacaa aatctgatac tttggagaca acactcaaga
     7501 atacgaagaa agcggcagcg cccattcaaa aactatcgag gcccaaaaaa ctgatagctt
     7561 ttggttggta cggcggcaag ttttctcatc ttgattggtt gctccctcat cttcctgaat
     7621 gcctgcatta ctgtgagcca ttcgcggggt cgggagcggt gttgctcaac agagagcctt
     7681 cgcctgttga aacctacaac gacatagatg gcgaggtggt gaattttttc aaggtattga
     7741 gagaagaaaa agaagcactc atcgagcaaa ttgcattgac gccattttca agagaagaat
     7801 ttgccgttgc ctgccaatta gaccccgatt tgacatcgtt ggagcgagcc agaaggtttt
     7861 atgttcgggc aaggcaggtc agaacaggtt tggctcaaac tgcctctgtt gggcggtggg
     7921 ccaattgtaa aaatacgagt cgggcaggca tgagcggagt tgtgagccgt tggctggggg
     7981 gcgttgaaca acttgatttt attgccgagc gattattgag agttcagatt gaaaatcgcc
     8041 ccgccataga tgtcattaaa ctttatgaca gcaaaaacac actgttttac tgtgatcctc
     8101 cgtacatcca tgaaacccgc ggcgatacaa aatcctatgg ttttgaaatg acggattcgg
     8161 atcacaagac attggcagaa acattgaacg ctgttgaagg tcttgcggct gtatctaatt
     8221 acgaatgcga actgatggaa gaactttatc catccggaaa gtggaccaaa atttacagca
     8281 ctgaaaagac cattcattct acaaaggata ttcgtcagga ggtactctgg gtaaactatt
     8341 caacttctcg tcttgaggcg ggcagcggaa gccgatagac ggtaaatcaa cgctaaaatc
     8401 gtcaatcttg tcagaactac aaaccatcaa acagcgcttc ggcatcatcg ggcaatcggc
     8461 actgctcgac cgcgccctca gcaccgccgt tcgcgtggcg cccaccgacc tcactgtgct
     8521 gctttccggc gagagcggcg tgggcaagga ggttttttcg cgggtcatcc actcgctcag
     8581 cgctcgcaaa cacaacaaat tcatagccat caactgcggc gctattcccg aaggcaccat
     8641 caattccgaa ttgttcggac acgaaaaagg ctccttcacc ggcgcggcca atgagcgcaa
     8701 aggctatttt gaagcttatg acggcggcac tatttttctg gatgagatcg gcgaaatgcc
     8761 tttagatacc caaagttacc tcctacgcgt actcgaaaac ggcgagttca tccgcgtcgg
     8821 ctcctctacc gtactcaaaa ccgatgtgcg cattgtggcc gccaccaatg tgaacctcga
     8881 agaacgcatc aaaaaaggca agtttcgcga agacctcttt taccgcctca ataccgtgcc
     8941 catcaaagtg cccgccctgc gcgagcgccc ggaggacatc tttctcctct tcagaaaatt
     9001 cgtggtggac ttctccgaaa aataccgcat ccagcccatt caattagatg aaaaagcccg
     9061 ccgtattctg gaaaactacg cttggcccgg caacatccgc gaactcaaaa acgtagccga
     9121 gcaggtctcc gtattagccg aagagcgcct cttggatgcc gaatccctcc tccgcgtagc
     9181 gcccatgatc ctacagcgcc acctgcccat gattgccgac aaaaatggct cggaaggcgg
     9241 cggtatgcag gagcgagaaa tcctctacaa gctcctcttt gatatgaagg gcgacctcaa
     9301 cgacctcaaa accctcgttt ttgaactcat ccgcagcaac gacctcaacg tgcccgatac
     9361 ccagcacctt cgcgtcttgc aatcgcccac cacttacgcg cccgccgaac ccgccgccat
     9421 gcacatagcg cccgaagcgc ccctcggcag ggccattgtg ccggtgcgcg aagaaagtta
     9481 cgacatgtcg gcgcctgcgg tgatgctgga acattcccgc catcacgacg acgactacga
     9541 aagcatggag gtattggaag aaaacctctc gctcatcgaa aacgaaaaag aactcattcg
     9601 caaggccctt cgtaaacatg ccggccgtcg caaagacgcc gcccaggacc tcggcatctc
     9661 cgagcgcaca ctgtatcgca aaatcaaaga atacgacatg caatgaggca gtttatcttt
     9721 tttacctcac tcatcgcggt actcgccgct tccggttgct ataccctcag cggcgtcagc
     9781 attgacccca atatttacaa aacctactat gtggagcagt ttgtcaacaa tgccgacaac
     9841 gcgccgccca ctttagccgt ggaagcctcc gagacgctca aggaaaaaat ccgcaccgaa
     9901 tcgcgccttg ttttcaacga caccgacccc gacattgagt tcaaaggcaa ggtcgtcagt
     9961 tacgtcgtca ctttcgaggc gccgcagccc ggcgagcagg ccgccatcaa ccgcctccgc
    10021 atcacctttg cagtggaata tatagaccac aaagaggaaa agaaaaactg gaaacaaaac
    10081 ttccaatttt tctttgatta tcccactaca accgacctcg cctccataca gtcgcaggcc
    10141 cacgaagcca tcctccgcca ggtcatggaa gacattttta ataaaacttt taccgattgg
    10201 tagtagctgt tatgctgctt gtgactggtt attgtgctta aaaagaggtt gctcttttta
    10261 cctttgcaaa aaaacaatca tggccaaacc aagcattgcc gaactcgaaa gccacatgcc
    10321 gcaaaccggc agcgtagcgt ggataggtat ccgtccgggc gtccggcagc cattaattgc
    10381 cgtggaaaaa gtagaagccg tagcgggcac aggtcttcgc ggcgaccact acgacaatcc
    10441 cggcggcaaa cgccaactca ccctcattca gggcgaacat ttagacgccg cagcctccat
    10501 cctctcacag tcggaaattg accccggcct cacccgccga aacatcgtgg tgcgcggcat
    10561 caatttgcag gcgctcaaag agcggcgctt cagcatcggc gaggtggtca tcgaagctac
    10621 gggcgactgt gtgccctgtt cgcgcatgga ggaaaacctc ggcacaggcg gctacaacgc
    10681 tatgtgcggg catggcggca tcacggcgcg catcgtttcc agcggccaca tcagcgtggg
    10741 cgacgaggta aaactgctgc cggaaaatgc ctaaaaccaa tgccgttcga ttgctcaacg
    10801 cggccggcat cgcttaccgt ttggtggaat accggtacga tgaggcgcag cttggcgtgg
    10861 acaaaatagc ccgcgacaat gggcttgagc tggagcgcat tttcaaaacg ctggtgctga
    10921 aaaccgaaac cgggcaggtc gtcgtggccg tcactccggg cgataaagca ttggatttca
    10981 aaacggtggc ccgcctcgct gcctgcaaaa aaatacactt ggccgaaatg aaagagttgc
    11041 agtcgcttac gggctacata cgcggcggct gctctccggt aggcatgaaa aaaaacttcc
    11101 ctgtttttat tgaccaatcg gcgctggaac acgaacgcat cctggtgaat gccggtgcgc
    11161 gcggcctgct catggaggta gctcccggcg aattgggcag cctctgccat gccgtttttg
    11221 aatacatcac cgcctgaatc tgccccgaca tggaaaccgc cgaactgctg cgcaaagtca
    11281 gaaaattgga aatcaaaacc aaacgcctga gccgccacct cttcgcaggc gagtatcaca
    11341 gcgcgttcaa agggcgcggc atgtcgttca gcgaggtgcg cagctaccag tttggcgacg
    11401 acgtgcgcca catagactgg aatgtgagcg cccgcacagg ccaaccgttt gtgaaggttt
    11461 ttgaagaaga acgcgaactg acggtgatgc tgctggtgga catgagcgcc tcttccgtct
    11521 ttggcgctgt ggcgcaatcc aaacgcgagt ttcagataga aatagcggcg ttgctggctt
    11581 ttgcggccat tcaaaacaac gacaaagccg gcatcatttt ttttaccgac gaggtggaat
    11641 tgttcatccc tccacagaag ggaaggcacc acattttgcg cattatcaga gagttggtca
    11701 attttgaacc ccgccgaaag ggcaccaaca tcgctgcggc gctggagttt ttcagccacg
    11761 tcatcaagaa gcggtccatc ggttttttaa tctcggattt tgttgccccc gactacgagc
    11821 acgcgcttcg cattgcggcc cggcggcacg aactcatcgg cattcaggtc ggcgatccct
    11881 tagagcggca actgcccgac gccggtttgt tgcacttcac cgaccctgaa accgggcgca
    11941 ccgcctggat ggacagctcg tcggcggagt tgcggcgcga gtatgccgca gcagcccaag
    12001 cgcgggatga ctatttcagg aatgcttttg caaaaggagg ggccgatgcg ctgcaaatcg
    12061 gtacggacga atcctacctt catgcgctga tgggattttt tcaaagacgc cgtattccgt
    12121 agagacgttg cacgctgtac agacgttgca tgcaacgtct ctacggtttc atcagggcgc
    12181 taccgttttt cttttgccct gcgtttgctc ggccatgcgc acggctgctt ccagatcgag
    12241 gatgccgccg gtggtggaga gcgatttgaa cggcactttg tcttcgccgc cggggatgat
    12301 cacatctata ttttgtttgg atgaggaact gacgaggatg tttttgacct gcaccgccgt
    12361 cagttgggga aagtacgaac gcagtacggc ggctgcgccg gccaccatag gcgcggccat
    12421 gctggtgccc tgaaggttgc ggtattggtt gtccacatcg gtggagtaga tgtccacgcc
    12481 gggggcgaag atgtccacat atttgctgct gtaattggaa aattcagcgg caagcatttc
    12541 accgcctttc cagtgagtgg cgcccacttc catccagttg gcggcgtatt ttgggccgaa
    12601 gaggcctttt ttgaggtagc tgcggttggg gaaattattt tgcaagtcaa tttcctgacc
    12661 atcgttgccg gcgccgtgta tgagcagcac atcgcgtttg gcggcatagc gcacggcacg
    12721 gtctatggcg gccttgtgcg gagaagcgcc cttgccgaag ctcatattga tgaccaaagc
    12781 gccattgtca acagcataca ggatggcatt ggccacgtct ttatcgtgct cgtcgccgtt
    12841 gggcacggtg cgcacagaca tgatacgtac gttgccggcc acgccatcca taccgatgtt
    12901 gttgttgcgt tgagcgccaa tgatgccggc gacgtgggtg ccgtgggtag catcggggcc
    12961 tttgacatcg ttgttgccat agcctttttc gtccacatcg gcgtagttgt cgcccactat
    13021 gttgcgcggg ttgtaatcca gattgtagtt gtaattgtac tcgtcatcca gatgcgtgta
    13081 gtactcttcg aggtcgcgct tgacatcgcc gagggtttgg ccgctgccac ccatcacgct
    13141 gagggctacg cgggtggcgc gggtgacgga aggtttgttg cttttgactt tgcgtacatc
    13201 ttcttcagag acctgaaagg ggtctttgcc aatgtgcttc accagatcgt tcagcgcttc
    13261 cacgacgggg ccatacaact ccatattttg cttggcctgc ttgcgctttt cggctatgac
    13321 gccctcgtat tctttatatt tttcgtagtc ctttttatcg gcggcgctga gtttggaagc
    13381 atcggcgttt ttgaaacggg catcgtaggc acggtattgg cgcacgactt cgaggttgtc
    13441 ggcgcccaca ttgcggccat ccttaccgcc gaggaaattc cagccgtgaa tatcgtcaat
    13501 gtatccgttg tcgtcgtcgt ctttaccgtt gccggcaatt tcgcccgggt tggtccacat
    13561 cacattgcgg aggtcttcgt gttcggcatc cacgccggaa tcaatgaccg ccacgatgac
    13621 ggtttgggag gttttacctt tcagaggccc ttcataagct ttgttggtgc gcaaaccggg
    13681 aaagccgtct ttggcaggat cgaggtgaaa ccagtcgagc ggaggtttct gcgcgccggc
    13741 cagagtggcg accnnnnnnn nnnaagaagg aggattgtga aaaaatgctt ctgcttcata
    13801 gttaagtaat gattttgaga atggatattt gtgccgcaat ttgaattttg catgcggcgt
    13861 tggagataaa cgtcaatgct gaccattgag atttatacac agacaaaatt tgtaccaaaa
    13921 agatgaaact actcaaaatt ttgatcttgc tgttgctgcc ggccttcgcc catgcgcaat
    13981 attgggaagc cggcgccttt gcggggctgt ccaattatct gggcgatcta agcgccaatt
    14041 cccaaaccat ttacatgcaa aacacaggct tcagcggcgc ggtctttgca cgttacaaca
    14101 tcaacgacct caccggagtg cgcctggcgc tgggctatgc aggcttgtcg ggcacagatg
    14161 ccttagccga cagcgaggct attcgcaccc gcaatctcag cttctcttcc gatgtgattg
    14221 aactggctct caccggcgaa ctgaacctgc cgggctatca gccatacaac ctcaaccggc
    14281 cgctttcgcc gtacctcttt gcaggcattt ctgcatttta tcacaatccc acggccaact
    14341 tgggcggcgc gaacatccgg cttcgaccgc tcggcaccga aggccaggga ttgcaagacc
    14401 ggccggcgcc ttattcttcc attgcattcg ctattccaat gggcctgggc gtaaaatatg
    14461 ccgtcactga ccggctcaac atcggtatag aattcggtgc aagaatgacc ttcaccgatt
    14521 atttggacga cgtgagcggc acctatctca gctttcccga actcttggca ggcaatggtc
    14581 ctctcgcagc ggccctcggc aatcggagcg gcgaactgct gggcggcgag cctgtcatcg
    14641 ttcctaccgg tactcctcgt ggcgacaatc gcgcccgcga ttggtttttc aacgccgggc
    14701 tgtctatttc ctacaatttc ttagacagcg gcttagtggg aagccgcaac agaatccgca
    14761 aaaaagcagg ctgcagaaca taagcagata aactgcgaag cagatcacca ctctccgcag
    14821 gcagagataa actgcgcaac agattactcc tctccgcagg cagataaact gcgcagcaga
    14881 ttactccgta ggagataaac cattactttt gcagcatcca aaccaaaaaa acattgagct
    14941 tatgtccaat tccaccatac gctacagctt cctgctcggc atgatcgctc tcgctgttgt
    15001 gagccgcatc ctgccgcacc cccacaattt tacgcccatc ggcgcagtcg ctcttttcgg
    15061 cgcagcctac ttcacgcgcc aatgggtagc tatgcttgct cctttcatcg ccctgtggct
    15121 cagcgactta tttatcaata acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn ncgctgtggg tgtatggcgc ctttgccctc atcaacctct tgggcatggc
    15241 agccctgcgt cgggtatcgg cagcgcgggt aggcggttcg gcatgggccg cgtcggtact
    15301 ctttttcatc gtcaccaatt tcggcgtttg ggcatcaggc ggcatgtatc ccaaaaacac
    15361 agaaggtctc atagcctgct tcgtcgccgg ccttcccttc ttcggcaaca ccatcatggg
    15421 cgatctgttt tacagtctcg tcctgttcgg cgccttcgag tgggcacaag cccgcaagct
    15481 cattcctgct gccgcctgac cgttttgcga aaaactcaac gcgtgccgga aaagccatct
    15541 cgcaccaaca acccaaatcc cgcgccactc cgcgaaggca ttgattacta tatcgaaaac
    15601 gggcgctacg tttttaccga acactttttg cgccaacgcg gctattgctg ccaaagcggc
    15661 tgccgccatt gcccttatgg gttccaaaag gaaataatac ctgcggagga gtaggtttcg
    15721 ggttggaacg actgctggct gcaagccaca agccgtgatt actacgctta gcaccgccta
    15781 ccgcacaccg catactggtt tcgggttcaa ggtttcgggt ttcatccgcc gaagccttgg
    15841 cgcaggcagg tttcgggttc aaggtttcgg
//
LOCUS       DAORLF010000044        15858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_1987_length_15858_cov_2.662912, whole genome shotgun sequence.
ACCESSION   DAORLF010000044 DAORLF010000000
VERSION     DAORLF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_15858_cov_2.662912"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1015)
                     /locus_tag="PK198_05975"
     CDS             complement(155..1015)
                     /locus_tag="PK198_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRF38312.1"
                     /translation="MKPIQLLLFFAFALLLNSAAAQPKTAYVLYDAAGKKVSYEKMIK
                     TLKDKDIVLFGEYHNNPISHWLQIETVMDLHPLRPLVLGAEMFETDNQEALDMYLAGT
                     INDKGLDSLARLWKNYPTDYAPLVRFAQKNKLPFAATNIPRRYASKVARGGFEALDTL
                     SDQEKAWIAPMPMPYDAELPGYKAMLEMMGGHGGANLPKAQASKDATMAHFILQYYRP
                     GALFIHFNGAYHSDNYEGILWYLRRQQPQLRYATISTVSQADNRKLLPENKGRADFII
                     CVDEDMTTTY"
     gene            1198..>2235
                     /locus_tag="PK198_05980"
     CDS             1198..>2235
                     /locus_tag="PK198_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRF38313.1"
                     /translation="MRTEWKLLSTDENAAESLRQALGIHPVLCRLLVQRGIHSFEQAK
                     AFFRPSLEDLHDPFLMQDMDAAVQRLRQAIEKGEKILLYGDYDVDGTCSIALMYSFLK
                     KLHPHLDYYIPDRYREGYGVSIEGIEYARENGAALIIAMDCGITAIDQVTRAHAYGID
                     FIICDHHLPGDALPEALAVLDPKRPDCAYPYKELSGCGVALKLAQAYSARYEVDASHW
                     QSLLDFLVISIAADIVPMTGENRVLAWYGLQQLNRSDKPGLNALIEESRRSRPLNVSD
                     IVFGLAPMINAAGRMADAGQAVRLMLAQDRLVAAEYSRVLEYRNQLRREYDLRTFEEA
                     EAMPAAHDETRR"
     assembly_gap    2236..2344
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2345..2892
                     /locus_tag="PK198_05985"
     CDS             <2345..2892
                     /locus_tag="PK198_05985"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRF38314.1"
                     /translation="VGSARSVAGFDLHAALGQCRDLLKSYGGHAHAAGLSLLPENVHA
                     FSERFEAVVRQSLSEDDIRKVISVHAEIDLQDITPAFGRVLKQFAPFGPGNRNPVFVT
                     KQVSDTGYSRLLKGDHLKLSLRQGERSMSGIAFGQGEALTKGLLQRPFDVCYTLEESQ
                     WDERREWELMVKDMRAGGGEK"
     gene            complement(2896..>4107)
                     /locus_tag="PK198_05990"
     CDS             complement(2896..>4107)
                     /locus_tag="PK198_05990"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRF38315.1"
                     /translation="EIVAEGRFVMPGIIDDQVHFRDPGLTHKADLYTEPRAAVAGGVT
                     SFMEMPNTKPPALTQALLEEKYAAAAQKSLANYSFFMGASNDNLDEVLRTNGRDVCGV
                     KAFMGSSTGNMLVDAPATLEALFSNVPLLIATHCEDEATVRRNEALWRERYGEDVPMS
                     EHPHIRSVEACLLSSSFAVELARKHNTRLHILHISTEEELALLRNDIPLEQKRITSEV
                     CVHHLYFNSDDYARFGSQIKCNPAVKAPRHQQALLPALLDNRLDIIATDHAPHTWEEK
                     QYSYFGAPSGVPLVQHSLNIMLEFYHQGRISMERIVEKMCHAPAQCFQIEQRGFLQEG
                     YWADVVILDPDREWTVTPDNIYYKCGWSPLLGHTFRGMVEASIVSGHLAYQNGRFFEG
                     KKGGRMLFERE"
     assembly_gap    4108..4132
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4294..4470
                     /locus_tag="PK198_05995"
     CDS             4294..4470
                     /locus_tag="PK198_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38316.1"
                     /translation="MNPNLRAAHFVSHISAIGKSAGKCQVGQAAEIQALKAALEAQRQ
                     DVARILAKLDAGQK"
     gene            4555..5982
                     /locus_tag="PK198_06000"
     CDS             4555..5982
                     /locus_tag="PK198_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF38317.1"
                     /translation="MLVGREKERGILERVLDSESSELVAVLGRRRVGKTFLIHSVFEG
                     KIRFETSGVKNATLREQLGNFHFRLKLSFGEHAPSKPPKTWQEAFFALTLCLENDVSQ
                     HQNVLFFDEVPWLATARSKFLNALSFFWNTWAVQHKVIVVLCGSAASWMLQKLINDKG
                     GLHNRITKRIELKPFTLRETEDFLKSRHINLDRYQTAMLYMALGGIPHYLNEVTKGKS
                     AHQNIQDVCFSPGGTLQSEFSRLYPALFDNPEHHIAVVRALAAKPTGMGRPEILAAVG
                     IPDGGAFSLCLEELEASGFISSYFSFGRKRKDMVYRLTDEYSLFFLKFIATRPNLDEH
                     IWTNLSQTQSYKAWVGYAFESLCLKHIVQIKKALGISGVYSESSAFYRSAQAGTEGVQ
                     VDLLIDRQDNVINLFEIKFHSEPYVLTKSAADGLRRKSALFRHYSQSSKHIFLNLLSP
                     RGLIENEHSLGLIDQALDIDALFTH"
     gene            complement(6105..7559)
                     /locus_tag="PK198_06005"
     CDS             complement(6105..7559)
                     /locus_tag="PK198_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF38318.1"
                     /translation="MKKRIRNIMVLMIVSIVGANLFQGYWLYQAYRLNHRDMNRHANM
                     ALGAAVSRTELSRAQDVLGSFDSVGQVVFHTIEKPMPEMRGEKRITLLGDSLTARRPN
                     DSDKTIHITLRETETQDSMRQYADTLARRISSMLVMNKMYEKGVDLKGIEKAYKEELR
                     TRNVEADFTLDTIRVASWKDHRYEISPAIKIGQSIETRIMPVNPLSKLGIQARFPHPA
                     PHILSRMIGTLIGSVVLLVVTTWAFIYMLRTILRQKKLSEIKSDFINNMTHELKTPIA
                     TVSAAVEALQHFNAMNDPAKAKDYLEISQQELNRLSGLVEKVLNIAVEERRELDLRPE
                     PVQVLALLEEVVQRYRIRNGKPIEIRLEHQLSEATAMLDRTHFTNAIHNLVDNAIKYS
                     YDSVCIIIRCTSEEGWLRISVSDNGIGIPKAYQHSIFEQFFRVPQGDLHNVKGFGLGL
                     AYVKKIVEKHGGRISVKSEPRHGSEFTIAIPVAA"
     gene            7714..>9580
                     /gene="pheT"
                     /locus_tag="PK198_06010"
     CDS             7714..>9580
                     /gene="pheT"
                     /locus_tag="PK198_06010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRF38319.1"
                     /translation="MFLLYFSDKMTISLNWLKAYLDIHLDPVRVGEVLTELGLELEGM
                     EEIESIKGGLRGVVVGHVLECGRHPNADKLSVTKVDIGAGAPLGIVCGAPNVAAGQKV
                     LVATVGTTLYPLGSEEPLVLKKAKVRGEESEGMICAADELGLGEDHSGILVLPADTPV
                     GMPAAEYFKVEKDVIYEIGLTPNRSDATNHVGVARDLAATLRINYGHTGQLCLPSVEH
                     FAVHNHELPIEVQVDNTDACPRYAGVCIQGVSIGESPDWLKNRLASIGIRSINNVVDI
                     TNFILHELGQPLHAFDYDAIAGRKVIVRTLPEGTPFQSLDEVERKLSAEDLMICDGNS
                     GGMCIGGVFGGLRSGVKDSTRNIFLESAHFNPKFIRRSSMRHNLRTEAARVFEKGSDP
                     NIALYALQRAALLICELAGGRIASEVIDLYPNPVQPKAVTLRNDHISRLIGIDISREE
                     IFAILDVLDMPRVAETADTITVTVPTNKSDVLREADVIEEILRIYGFNKVPIPAQIRT
                     AAAEAPKPDPFQIRNAVGDYLAAAGFNEMMALSLSESRFYKEILPTVADEELVYINNT
                     SNVYLDIMRPTMLFSALEAAVHNQNRRQTDIRLFEFGKTYRMKGEGIAEHAHLSLL"
     assembly_gap    9581..9609
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9610..10197
                     /locus_tag="PK198_06015"
     CDS             <9610..10197
                     /locus_tag="PK198_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRF38320.1"
                     /translation="ESWIEKPQRSVDFYTLKTFVVNILARLGANAYQESPVDNAAFAY
                     GVRYHRGERILAELGKVAGPTAQAMGLKSEVFFADIRWDALMDAVAKQKINYREVGKF
                     PSVRRDLALIVENSVKFSDIAAIAGKAGKGLLKDVNLFDVYENEQAVGKGKKSCAVSF
                     VFEDAAKTLQDKDVEKIMDQLIREYETKLGALIRR"
     gene            10246..10545
                     /locus_tag="PK198_06020"
     CDS             10246..10545
                     /locus_tag="PK198_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38321.1"
                     /translation="MTLSGRVDDMALKMMRVAELMQQLREENHALRTANEQLAARLDA
                     AVVALEQARALPTEEERQETGGPERPTTELSDKLKEDIEHYIAEIDKCIDWLRNH"
     gene            10551..10850
                     /locus_tag="PK198_06025"
     CDS             10551..10850
                     /locus_tag="PK198_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRF38322.1"
                     /translation="MKEEDEDIKNITVYVTGRPYPLRISTADELVIRKIVKEVNDKIS
                     HFQLTYTHKDMQDCVTMAVLTYAVELYKSSSEASENPGLEHSLSRLENLLDELIA"
     gene            10974..12656
                     /gene="rny"
                     /locus_tag="PK198_06030"
     CDS             10974..12656
                     /gene="rny"
                     /locus_tag="PK198_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRF38323.1"
                     /translation="MDPILGLIIGLIAGAVGSFFVVRGQQNKTANKHQEEAKALAERE
                     LEEARLNVKRIVTEAETKAEKLISQAEINNERTKQRKIQEAQERYSKMRSEFDKEKNA
                     HVVEMKERELEVKAQEAELKVKQDKFSERVEQIEAQKAELEAKDTELNAERESLEKQL
                     KIVAKRKEELDAANEIRIKELETIARMSEAEAKEQLLEAVRAKSETEAMSIVKASIEQ
                     AKTDANKEAKKIVIQTIQRMCAEYTIENTVSVFNLDSDELKGQIIGREGRNIRALEAA
                     TGAEIVVDDTPEAIVISSFDPIRREVARLALKRLVADGRIHPARIEEVVAKVRKELDQ
                     QVIEIGERTVIDLDLHGLDPYLVKMVGRMRFRSSYGQNLLKHSIETANLCAIMAAELG
                     LNPKQVKLAKRAGLLHDIGKVAEEESELSHAILGMKLCEKHKEHPAVINAVGAHHDEI
                     EMNNIISPIIQACDAISGARPGARREILESYLKRIGELEDLAMAHEGVSKAFAMQAGR
                     ELRVIVEAEKVSDQYADELSFMISQKIQNEMQYPGQIKVTVIREKRAISFAR"
     gene            12918..13706
                     /locus_tag="PK198_06035"
     CDS             12918..13706
                     /locus_tag="PK198_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HRF38324.1"
                     /translation="MTVKDKNVAGILAFFLGGLGAHRFYLDQVGLGIFYLIFFWFPLT
                     WLIGVIDAIIFFTMKQEKFDAKYNYGKQRGQEADPRRRQQEYSRPQRAPQRQPRQEPQ
                     TPPRRQQGSPEIKAHELKSAGIKKFKDYDYDGAIADFEKALQIAPKDVAIYFNLACAY
                     SLNEQADKAFQHIDKAVALGFNDFQRIKEHDALAFLRIQPDFEAFEKNGFQLVQKLAP
                     PPQPDLLQQMSGDLLEQLNRLGDLREKGLLSEDEFAAQKRKLLG"
     assembly_gap    13790..13882
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13932..>14125
                     /locus_tag="PK198_06040"
     CDS             13932..>14125
                     /locus_tag="PK198_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF38325.1"
                     /translation="MIAEELITDSIVPLRTSDTGDEALGIMAEFYVKHLPIVNNTQLL
                     GLVSEDDLLHHDPEEPVGAM"
     assembly_gap    14126..14135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14136..>14525
                     /locus_tag="PK198_06045"
     CDS             <14136..>14525
                     /locus_tag="PK198_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF38326.1"
                     /translation="LSLNRPYVHSNDHFYEVLRMMALHQLSVIPVVDEEDNYLGLITL
                     EDLLQHFARTGGFAEPGSIIVLETSKRGYSLAEIARIVESENASVLNSFVGTSPDGST
                     VEVTIKINAHNNNSILATFERFGYEVKA"
     assembly_gap    14526..14619
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14825..>15858)
                     /locus_tag="PK198_06050"
     CDS             complement(14825..>15858)
                     /locus_tag="PK198_06050"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38327.1"
                     /translation="NACGASSPSVLNVNVSPTPVQPGAISGNTSVCQGSAQTYSISAV
                     VGATSYTWTLPSGWSGTSTSTSISATAGSSGGAIFVTANVSCGNSSPSTLTVNVTPTP
                     SQPGPISGNTSSICQGSTQIYSIAPIPEATSYYWTLPFGWSGNSSTNSITTTAGTFGG
                     IFSVSAINSCGVGAAQTISVSTTVIDTFINVNGITLTANAASASYQWVNCPGLDSILG
                     ENGRSFTPMQNGNYAVVVTLNGCSIVSGCHEVTITSVSESEGGSTMEVFPNPTSGFLT
                     IKGSGLKDNKYQILLFNAMGQEMDRKDFLVVNNDLEANLFIEHLPAGPYMLIIKSLKT
                     HYVFKVLKQ"
BASE COUNT         3767 a         4144 c         4159 g         3428 t
ORIGIN      
        1 gctttgtagt cggctttggg gccgatgccc aataatgctt tgatggcggt caacatggtc
       61 gtgttctgat tttggatgca aaaaagcagt gttctgcatc tcccggaagg tgaccgaggt
      121 tacattgggc aaatctttta cggaaaaaaa accctcaata cgttgtcgtc atatcctcat
      181 ccacacaaat gataaaatcc gcgcggccct tgttttcggg caacagcttg cggttgtcgg
      241 cctgcgagac ggtagaaatg gtggcatagc ggagttgcgg ctgctgccgg cgcaggtacc
      301 agagaatgcc ctcgtagttg tcggaatgat aggccccgtt gaaatgtatg aacaaggccc
      361 ccggacgata atattgcaaa atgaaatgcg ccatcgtagc gtctttgcta gcctgcgctt
      421 tgggcaagtt ggccccgccg tgaccgccca tcatctccag catggctttg tagccgggca
      481 attccgcatc gtagggcatg ggcatcgggg ctatccaggc tttttcctga tcgctgaggg
      541 tgtctaaggc ttcaaaaccg ccgcgcgcca ctttggaagc atatcggcgc gggatattgg
      601 tggcggcaaa aggcagtttg tttttttggg caaaccgaac caaaggcgcg taatcggtgg
      661 ggtagttttt ccacaggcgg gccagcgagt ccaaaccttt gtcgttgatc gttccggcca
      721 agtacatgtc caaggcctcc tgattgtcgg tttcaaacat ctcggcgccc agcaccaaag
      781 gccgcagcgg gtgcaggtcc atcacggttt caatttggag ccagtgagag atgggattgt
      841 tgtgatactc gccgaacaaa acgatatctt tgtctttcag tgtcttaatc attttttcgt
      901 agctcacctt tttgccggcg gcatcgtaca aaacgtaggc ggttttgggt tgcgcggcgg
      961 cggagttgag caataaggca aaagcgaaga acagcagtag ttggattggt ttcacggtag
     1021 atgattttga gacaaaagta aaattttttc tgaggtctta tggaatccgg catgtttcgc
     1081 cgtctatatc aaagctgcga gtttgattct tgcttattta gtgaaggggt gactatcaga
     1141 ttgagtgcct ggctaagtca ccccttcact aaacacatca cgaatatcac caccgccatg
     1201 cgcaccgaat ggaaattgct gagtaccgat gaaaacgccg cagaatcgct ccggcaggcg
     1261 ctgggcattc acccggtgct gtgtcgcttg ttggtacaaa ggggcattca cagcttcgag
     1321 caggccaagg cttttttccg gccttcgctc gaagacctgc acgatccttt tcttatgcag
     1381 gatatggatg cggcggtaca acggctccgg caggccattg aaaaaggtga aaaaatcctg
     1441 ctctacggcg actacgacgt ggacggcacc tgctccattg cgctgatgta cagctttttg
     1501 aaaaaattgc acccgcatct cgactactac attcccgacc gataccgcga gggctacggc
     1561 gtttcgatcg aaggcattga gtatgcccgc gagaacggcg ccgcactcat catcgctatg
     1621 gactgcggca ttacggccat agatcaggtg acgcgcgccc atgcttacgg catagatttc
     1681 atcatctgcg atcaccacct gccgggtgac gccctgcccg aagccttggc cgtattagac
     1741 cccaaacgcc ccgattgcgc gtatccgtac aaagaactca gcggctgcgg cgtggccctc
     1801 aaactggcgc aagcctactc tgcccgctac gaggtggacg cgtcgcactg gcagtctctg
     1861 ctcgattttt tggtcatcag catcgccgcc gacatcgtgc ccatgaccgg cgaaaacagg
     1921 gtgctggcct ggtacggatt gcagcaactc aaccgcagcg acaagcccgg actgaacgcg
     1981 cttattgagg aaagtcgccg ctcgcgcccg ctcaacgtca gcgatattgt gttcggactg
     2041 gcgccgatga ttaatgccgc cgggcgcatg gccgacgccg ggcaggctgt gcgattgatg
     2101 ctggcccaag accgcctcgt ggccgccgag tacagccggg tgctggaata ccgcaaccaa
     2161 ctgcgccgcg aatacgacct ccgcaccttc gaggaagcag aggccatgcc tgctgcacac
     2221 gacgaaacgc gccgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnncagtgg gctcggcgcg ctcggtggcg ggcttcgatc ttcatgcagc gctggggcag
     2401 tgccgggatt tgctgaaaag ctatggcggc catgcacacg ctgccggact gagtctgctg
     2461 cccgaaaatg tgcatgcttt cagcgagcga ttcgaggcgg tggtgcggca gtctttgtcg
     2521 gaagacgata ttcgcaaagt catttccgtc catgcagaga ttgatttaca ggacattacg
     2581 cctgcattcg ggcgggtgct gaaacaattt gccccctttg gccccggcaa ccgcaatccg
     2641 gtgttcgtca ccaagcaagt gagcgatacc ggctactcgc ggctgctcaa gggcgatcac
     2701 ctcaaactat cgctccggca gggcgagcgc agcatgtcgg gcatcgcgtt cgggcagggc
     2761 gaggcgctga cgaagggcct gctccagcga cctttcgacg tgtgttatac tttggaagaa
     2821 agccaatggg acgagcggcg ggaatgggaa ctgatggtga aggatatgcg ggcgggagga
     2881 ggagaaaaat gattttcact ccctctcaaa cagcattctt cccccctttt ttccttcaaa
     2941 aaaacgtccg ttttggtacg ccagatggcc actcacaata gaagcctcca ccatgccccg
     3001 gaaggtatgc cccagcaacg gagaccagcc gcatttgtaa taaatattgt cgggcgtcac
     3061 cgtccattcg cggtcggggt cgaggatcac cacatcggcc cagtagcctt cctgcaaaaa
     3121 tccacgctgt tctatttgaa agcactgcgc cggagcatgg cacatttttt ccacgatgcg
     3181 ctccatggaa atgcgcccct gatggtaaaa ttccagcatg atattgagcg aatgttgcac
     3241 gagcggcacg cccgacggcg ctccgaaata cgaatattgc ttttcctccc atgtatgcgg
     3301 cgcgtggtcg gtggcgatga tgtcgagccg gttgtcgagc agagcgggca gcagcgcttg
     3361 ctggtgtcgg ggcgctttta ccgccgggtt gcacttgatc tgcgagccga agcgggcata
     3421 atcgtcgctg ttgaaataga ggtgatgcac gcatacctcg gaagtgatgc gcttttgctc
     3481 caacggaata tcgtttcgaa gaagggcaag ttcttcctct gtggaaatgt gcagaatgtg
     3541 caggcgggtg ttgtgcttgc gcgccagttc caccgcaaaa gaggacgaaa gcaggcaggc
     3601 ctccacgctg cgaatgtgcg ggtgttccga catgggcacg tcctctccgt agcgttcgcg
     3661 ccacagcgct tcgttgcggc gcacggtggc ctcgtcttcg caatgcgtgg cgatgagcag
     3721 cggcacattg gaaaacaatg cctccagcgt agcgggcgca tccaccagca tattgccggt
     3781 actggaaccc atgaacgcct tcacgccgca cacatcgcgg ccattggtgc gcaacacctc
     3841 gtccaaattg tcattggagg cgcccataaa aaaagagtaa ttggccagcg atttttgggc
     3901 ggctgcggca tatttttctt ccagcagcgc ctgtgtgagt gccggcggct tggtattggg
     3961 catctccatg aaggaggtga cgcctcccgc tacggcagct ctcggttcgg tgtagaggtc
     4021 ggctttgtgg gtgagaccgg gatcgcggaa gtgtacctga tcgtctatga tgccgggcat
     4081 gacgaagcgg ccctcggcta caatttcnnn nnnnnnnnnn nnnnnnnnnn nngtcaatga
     4141 tgccgccgat gcgctcgatg cgcccgtttc tgatgagcag atcgccgtgg cggatggcgc
     4201 cgcgatcaac gatggcgccg ttttttatga gaatggaaga catggccgtg aatgattttt
     4261 gagcttcaaa gataagcatt tcggcgctga ccgatgaatc caaacctccg ggcggcgcac
     4321 ttcgtcagcc acatctctgc aattgggaaa agcgccggca agtgtcaggt cgggcaggcc
     4381 gccgaaatcc aggcgctcaa agccgccctc gaagcgcagc gacaggacgt ggcgcggatt
     4441 ttggccaagc tggatgcggg acaaaagtag tttttgtcaa aaaatgcgcc ataattcgcg
     4501 gaatcgtgtt atttttgcca cattccgcga atttaaaaca aaaaaaagac aaccatgttg
     4561 gtcgggcgcg aaaaagaaag gggcatattg gagcgtgtac ttgactcgga aagctccgaa
     4621 ttggtagccg ttttaggcag gagacgggta gggaaaacct tcctcattca ttctgttttc
     4681 gaaggtaaaa tacgatttga gacctctggg gtaaaaaacg ctaccctccg ggagcaattg
     4741 ggcaattttc attttcgcct caaattgtct tttggagagc atgctccttc caaacctccc
     4801 aaaacctggc aggaggcttt tttcgccctc acgctctgct tagagaacga tgtatcacag
     4861 catcagaacg tgttgttttt tgacgaggtg ccttggttgg ccaccgctcg ctccaaattt
     4921 ttaaatgcgt tgagcttttt ttggaatacc tgggccgtgc aacacaaggt gatcgtggtg
     4981 ctctgtggtt ctgctgcttc gtggatgttg caaaagctca tcaacgataa agggggattg
     5041 cacaatcgaa tcacgaaacg aattgagttg aagcctttca cgctgaggga aacggaagat
     5101 tttctaaaaa gcaggcacat taatttagac cggtatcaaa cggccatgct gtacatggca
     5161 ctgggcggca tcccgcatta tctcaatgaa gtgaccaaag gtaaaagtgc tcatcaaaac
     5221 attcaggatg tgtgcttttc tcccggcgga acgttgcaaa gtgaattttc acgattgtat
     5281 cccgcattgt tcgacaatcc ggagcatcat atcgccgtcg ttcgtgcatt agcggcgaaa
     5341 ccaacaggca tggggcgccc ggaaatcctc gccgctgtcg ggatacctga cgggggcgcg
     5401 ttttctctgt gtttggaaga attggaagca tccgggttta tttcttccta tttcagtttc
     5461 gggcgcaagc gcaaagacat ggtgtaccgg ctcaccgacg agtactccct gttttttctc
     5521 aaattcatcg caacccgccc caatctggac gaacacatct ggaccaacct cagtcaaacg
     5581 caatcttaca aagcgtgggt ggggtatgct tttgagtcgc tttgcctcaa acacattgtc
     5641 caaataaaaa aagcgctggg catatcgggc gtttattcgg agtcctcggc cttttatcgc
     5701 tctgcccagg ccggcacgga aggagttcag gtggacctgc ttatagaccg acaggacaat
     5761 gtcatcaatc tgttcgaaat caaatttcac tccgagcctt atgtgctgac aaaatcagca
     5821 gcagatggat tgcgtcgcaa gtcggcacta tttcgtcatt attcccaaag ctccaaacac
     5881 atattcctca acttactgtc gccccgcggc ctcatcgaaa acgaacatag tctcggcctc
     5941 attgaccaag cgcttgatat agacgctctg tttacacact aagtagtggg gcaaaaataa
     6001 atgttcattt tctttaggcc aaaggattag ataagacact taaaatgaaa cgtttactta
     6061 tctaatcctt tggcgaaaat gatcacttta tttttttatc gcccctaagc agctacaggg
     6121 atggcgatgg taaactccga gccatgccgg ggttcacttt tgacactgat gcgcccgccg
     6181 tgtttttcca ctattttttt gacgtaggcc aagccaagtc caaagccttt gacgttgtgc
     6241 aaatcgccct gtggcacgcg gaaaaactgc tcaaaaatgc tgtgctggta ggctttgggg
     6301 atacctatcc cgttgtcgct cacagagata cgaagccaac cctcctcact tgtgcagcgt
     6361 atgatgatac aaacggaatc gtaagaatat ttgatggcat tgtccacgag gttgtgaatg
     6421 gcgttggtga aatgcgtccg gtcgagcatg gccgttgcct cggaaagctg gtgttcgaga
     6481 cggatttcga tgggtttgcc gttgcggatg cgatagcgct gcaccacttc ttccagcaag
     6541 gcgagcacct gcaccggctc agggcggagg tccaattccc ggcgttcctc aacagcgata
     6601 ttgagcactt tttctaccaa tccggagagc cggttgagtt cctgttgcga aatttccaga
     6661 tagtctttgg ctttggcggg gtcgttcatg gcattgaaat gctggagcgc ttccacggct
     6721 gccgacacgg ttgcgatggg ggttttcagc tcgtgggtca tattgttgat gaaatcgctt
     6781 tttatttcgg agagtttttt ctgacgcaaa atggtgcgca gcatataaat gaatgcccag
     6841 gtagtgacca ccagaagaac aacgctgccg atgagcgtgc cgatcatgcg cgaaaggatg
     6901 tgcggcgccg ggtgcgggaa gcgagcctgt atgccgagtt tgctcagcgg gttgacgggc
     6961 atgatgcgcg tttcgatgga ctgcccgatt ttgatggccg gcgagatttc gtagcgatgg
     7021 tctttccagg aagccacgcg aatggtgtct aacgtaaaat ccgcctctac atttcgggtg
     7081 cgcagttctt ctttataggc tttttcaatt cctttgaggt ccactccttt ttcgtacatc
     7141 ttgttcatta ccagcataga agagatgcgg cgggccaggg tatcggcata ctggcgcatg
     7201 ctgtcctggg tttctgtctc gcggagtgtg atgtggatgg ttttgtcgga atcgtttggg
     7261 cgccttgcag tcagggagtc gccgagcagg gtgatgcgtt tttctccgcg catttcgggc
     7321 atcggctttt cgatggtgtg aaaaacgacc tggccaacgg agtcaaaact gcccagcacg
     7381 tcttgcgccc ggctgagttc agtgcgcgat accgcagcgc cgagtgccat attggcatgg
     7441 cggttcatgt cgcggtgatt gaggcggtag gcctgataca gccagtagcc ctgaaacaga
     7501 ttggcgccga cgatgctcac gatcatcagt accataatat tgcgaatgcg ttttttcacg
     7561 acggcgtccc ttgttgtatt gcaaaaataa cagcttttgg cgggcttcgg cgaagcgctt
     7621 aacacaaatt aacggtaaaa aaatcggcct ctcactttcc acccaaaacc cctatctttg
     7681 ccccgtttta ttgggcggtg caaaatgccg tttttgtttt tactttattt ctctgataaa
     7741 atgaccattt ccctcaactg gctcaaagca tatcttgaca tccatctcga ccccgtccgc
     7801 gtgggtgaag tcctcaccga actcggcctc gaactcgaag gcatggagga aatcgaaagc
     7861 atcaaaggcg gtttgcgcgg cgtcgtagtg ggccatgtgc tcgaatgtgg ccgccatccc
     7921 aacgcagaca agctctccgt caccaaagtg gacatcggcg ccggcgcccc gctcggcatc
     7981 gtctgcggcg cgcccaatgt ggctgccggc caaaaagtgc tggtggctac cgtgggtact
     8041 acgctctacc cattgggcag cgaggagcct ttggtgctga aaaaagccaa agtgcgcggc
     8101 gaagaatcgg aaggcatgat ctgcgccgcc gatgagctgg gcctcggcga agaccacagc
     8161 ggcattttgg tactgcccgc tgatacgccc gtgggcatgc ccgccgccga atattttaag
     8221 gtggaaaaag atgtgattta tgaaatcggc ctcacgccca accgttccga cgccaccaac
     8281 cacgtcggcg tggcccgcga tctggccgcc accctccgca tcaattacgg ccacaccggt
     8341 caactgtgcc tgccttccgt ggagcacttc gccgtacaca atcacgaact gcccatcgag
     8401 gtgcaggtgg acaacacaga tgcctgcccg cgctacgccg gcgtctgcat tcagggcgtc
     8461 agcatcggcg aatcgcccga ctggctcaaa aaccgcctcg cttccatcgg catccgttcc
     8521 atcaataatg tggtggacat taccaacttt atcctgcacg agctggggca gccgctccac
     8581 gctttcgatt acgacgccat cgccggccgc aaggtcatcg tacgcacctt acccgaaggc
     8641 acgccgttcc agagtctgga cgaggtagag cgcaaactca gcgccgaaga cctgatgatc
     8701 tgcgacggca actccggggg gatgtgcatc ggcggcgtat tcggcggact gcgttcgggc
     8761 gtcaaagaca gcacccgcaa tattttcctc gaatcggcgc atttcaaccc taagttcatc
     8821 cgccgcagca gcatgcgcca caacctccgc accgaggccg cccgcgtatt cgaaaaaggc
     8881 agcgatccca acatcgcgtt gtacgccctc caacgagcgg ccctgcttat ctgcgaactc
     8941 gccggcgggc gcatcgcttc cgaggtcata gatttgtacc ccaatccggt gcagcccaaa
     9001 gcggtgacct tgcgcaacga ccacatcagc cgcctcatcg ggattgacat ctcccgcgag
     9061 gaaattttcg ccattcttga cgtgctcgac atgccccgtg tggccgaaac cgccgacacg
     9121 ataacggtga cggtgccgac caacaaatcg gacgtgctgc gcgaagccga cgtcattgaa
     9181 gaaattctgc gcatttacgg gttcaacaaa gtgcccattc ccgcacagat acgcactgct
     9241 gccgccgaag ctccgaagcc cgatcccttc cagattcgga atgcagtggg cgattatctg
     9301 gccgcagccg gttttaatga aatgatggcc ctgtcgctgt cggaatcgag gttttacaaa
     9361 gaaatactgc ccaccgtggc cgatgaggaa ttggtgtaca tcaacaatac ctccaatgtg
     9421 tatttggaca tcatgcggcc cacgatgctg ttcagcgcgc tggaagctgc cgtacacaac
     9481 caaaaccgcc gacagaccga catccggcta tttgaattcg gcaaaaccta tcgcatgaaa
     9541 ggtgaaggca tcgccgaaca cgcccacctg agcttgttgc nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnng aatcctggat tgaaaagccg cagcgcagcg ttgattttta caccctcaaa
     9661 accttcgtgg tcaatatttt ggcacgattg ggcgccaatg cgtatcagga atcgccggtt
     9721 gacaatgccg cctttgctta cggggttcgc tatcatcgcg gcgaacgcat actggccgaa
     9781 ctgggcaaag tggccggccc tacagcccag gctatgggat tgaaaagcga ggttttcttt
     9841 gccgacattc gctgggacgc cctgatggac gctgtggcca agcaaaaaat caactatcgg
     9901 gaagtaggga aatttccttc tgtgagaaga gacttggccc tgatagtaga aaattccgta
     9961 aagttttcgg atattgccgc gattgccgga aaagcaggca aaggcttgct caaagacgtc
    10021 aacttgttcg atgtgtatga gaacgaacag gcggtcggaa aaggcaaaaa atcgtgtgcc
    10081 gtgagcttcg tttttgaaga cgcggccaaa actttgcaag ataaagacgt ggaaaaaatc
    10141 atggatcagc tcatccggga atacgaaaca aaactcggag cgctcatccg gcgttaatct
    10201 gcgtaattca ttgaaagccc gaagcaaggg cctcaaatcg aaagcatgac tttatccggg
    10261 cgagtggacg atatggcgct gaaaatgatg cgcgtcgccg agttgatgca acagctccgc
    10321 gaagaaaacc acgccctgcg aaccgccaat gagcaactcg cagcgcgatt ggatgccgcc
    10381 gtcgtcgctt tggaacaagc gcgtgcttta ccaacagagg aggaacgaca ggaaacgggg
    10441 gggccggagc gcccgacaac cgagctttcg gacaagctca aagaagatat cgagcattat
    10501 attgccgaaa ttgacaagtg tatagattgg ctgcgcaacc actgagccgt atgaaagagg
    10561 aagacgaaga tattaaaaac atcaccgttt atgtgacggg ccgaccgtac cctctccgca
    10621 tcagtacagc cgacgaactg gtcattcgca aaatcgtgaa agaggtgaat gataaaatca
    10681 gccacttcca gctcacctac acgcacaaag acatgcagga ctgcgtgacg atggcggtgc
    10741 tcacttatgc agtcgagctg tataaatctt cttccgaagc atccgaaaac cccggcctcg
    10801 aacattccct gtctcgtttg gaaaatctgc tcgacgaatt gatcgcgtag tcctttctga
    10861 ttctcctcct ttggaatatc aatcaaactc gtagctcaag ccacgcaacg gcatggtcac
    10921 agctacctga ccaactatat ttttaaaatc aacaacagta aaccacaaca cacatggatc
    10981 caattttagg actcataata ggcttgatag ccggagcggt cggcagcttt tttgtcgtcc
    11041 gcgggcagca aaacaagact gccaataaac atcaggaaga ggcgaaagct ttggccgaac
    11101 gcgagttgga agaagcccgc ctcaacgtca aacgcatcgt gaccgaagct gaaaccaaag
    11161 ccgagaaact catttctcag gctgagatca acaacgaacg caccaagcaa cgcaaaattc
    11221 aggaagcaca ggagcgctac tccaaaatga ggtcggagtt cgacaaggag aagaacgctc
    11281 atgtagtgga aatgaaagaa cgcgaactgg aagtgaaggc tcaggaagcc gaactgaaag
    11341 taaaacaaga caaattctca gagcgcgttg agcaaattga ggcgcaaaaa gccgaactgg
    11401 aagccaaaga taccgaactg aacgccgagc gcgaaagcct cgaaaagcag ctcaaaattg
    11461 tggccaaacg caaggaggaa ctcgatgctg ccaacgaaat ccgcatcaag gaactcgaga
    11521 ccattgcccg catgagcgag gccgaagcca aagagcaact gctggaagcc gtgcgcgcca
    11581 agtcggaaac cgaggccatg tccatcgtca aagcctccat cgagcaggcc aaaaccgacg
    11641 ccaacaaaga agccaagaag atcgtcatcc aaaccattca gcgcatgtgc gccgaataca
    11701 ccatcgaaaa cacggtgtcg gtgttcaact tggattcgga cgaactcaag ggccagatca
    11761 tcggtcgcga aggccgcaac atccgcgccc tcgaagccgc taccggcgcc gaaatcgtgg
    11821 tggacgacac gcccgaagcc atcgttattt ccagcttcga ccccatccgc cgcgaggtgg
    11881 cccgcttagc gctcaagcgt ttggtggccg acggtcgcat ccacccggcc cgcatcgaag
    11941 aagtggtggc caaggtgcgc aaagaactcg atcagcaggt catcgaaatc ggcgagcgca
    12001 cagtcattga cctcgacctg cacggcctcg atccttattt ggtaaaaatg gtaggacgca
    12061 tgcgtttccg ctcgtcttac ggccaaaacc tcctcaagca ctccatcgaa acggccaacc
    12121 tctgcgccat catggccgcc gaactgggcc tcaatcccaa gcaggtcaaa ttggccaaac
    12181 gcgccggtct gctccacgac atcggcaaag tggctgaaga ggaatctgaa ctctcgcacg
    12241 ccatcctcgg catgaagctc tgtgaaaagc acaaagagca tcccgcagtc atcaacgcag
    12301 taggcgccca ccacgatgag attgagatga acaacatcat ttcgcccatc atccaggcct
    12361 gcgacgccat ttccggcgca agacccggcg cccgccgcga aattctggaa agctacctca
    12421 agcgcatcgg cgaactcgaa gacctcgcca tggcgcacga gggcgtttcc aaagcctttg
    12481 ccatgcaggc gggccgcgag ttgcgcgtca tcgtggaagc cgaaaaagtg agcgaccaat
    12541 acgccgacga gctgtcgttt atgatttctc agaaaatcca gaacgagatg cagtatcccg
    12601 gccagatcaa agtgacggtg atccgcgaga agcgggccat ttcctttgcg aggtaatgga
    12661 attatagcag tatgcttcct ctgtgccgca agctgtaagc cgtaagctat acgcttaagg
    12721 cttgcggctt gcagccatga ggagtacgct gatattttta tggcgagtac gacaaccttt
    12781 gcacaccggc gcgcatttac acagggaatt ggcgtcaaca acaggcgtta tcggttgctg
    12841 cgtctaaaaa aacgcagcgt tgataggaat gaggggcatc ttacgacccg aattcaaacg
    12901 aacgaaaaat accattgatg accgtgaagg acaaaaacgt agccggtatt ctggccttct
    12961 ttctgggggg attgggcgcc caccgcttct atctggatca ggtgggactg ggcatttttt
    13021 acctcatctt tttctggttt ccgctcacct ggctcatcgg cgttattgac gccatcatct
    13081 tcttcaccat gaagcaggag aagtttgacg ccaagtacaa ctacggcaag caacgcggtc
    13141 aggaggccga tccccgccgc agacaacagg aatacagccg ccctcaacgc gcgccgcagc
    13201 gacagccccg tcaagagccg caaacgccgc cgcgccgtca gcaaggttcg ccggaaataa
    13261 aagcgcacga actgaaaagt gccggcatca aaaaattcaa agattacgac tacgacggcg
    13321 ccattgccga ctttgaaaag gccctccaga tagcgcccaa agatgtggcc atttatttca
    13381 atctggcttg tgcatactcg ctcaacgagc aggcagataa ggcattccaa cacattgaca
    13441 aggccgtggc actgggtttc aacgattttc aacgcatcaa agaacacgac gcgctggctt
    13501 tcctgcgcat tcagcccgat tttgaggcat ttgaaaaaaa tggctttcaa ttggtgcaaa
    13561 aattagcgcc gccgcctcag cccgacctct tgcaacaaat gtcgggcgac ctgctggaac
    13621 aactcaaccg tctcggcgac ctgcgcgaaa agggcttgct ctcggaagat gagtttgctg
    13681 cgcaaaagag gaagttgctg gggtgattca gtaggcggta ggcagtgggc ggtagccgcg
    13741 ccgtggcgcg gtaagcgcga ggaaacatgg aaccttctcc tgcttagtgn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nntcggcgga tgaaacccgg aatgaacaag aaacacggaa
    13921 tttgaaaagc catgattgca gaagaactca tcacagacag catcgttccg cttcgcacct
    13981 ccgacaccgg agatgaagcg ctgggcatca tggccgaatt ttacgtcaag cacctgccca
    14041 tcgtcaacaa tacccagttg ttgggcctcg tatcggaaga cgacctgctg caccacgacc
    14101 ccgaagagcc ggtgggcgct atggannnnn nnnnnttatc gctgaaccgc ccttacgttc
    14161 attccaacga ccatttctac gaagtactcc gcatgatggc cctgcaccag ttgtcggtca
    14221 ttccggtggt ggacgaagaa gacaactacc tcggtctcat tactttggaa gacctgctcc
    14281 agcactttgc ccgcacagga ggtttcgccg agccgggcag catcatcgtg ttggaaacga
    14341 gcaagcgggg ctactcgctg gcggaaattg cccgcattgt ggaatcggaa aacgcctctg
    14401 tactcaattc ctttgtcggc acctctcccg acggttctac ggtggaagtg accatcaaaa
    14461 tcaacgccca caacaacaac tccatcttag ccactttcga gcgcttcggc tacgaagtga
    14521 aagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna attctcccgg tgtgtggggg
    14641 cagtggcacg aggcgctgag tacgcatccg gcggatttgg agggggtgcg gggcggggta
    14701 gggatccacc tcgcgggtag cccccctgta tagtacacgt cacacaatgc gaagcaaaca
    14761 tgaggtgcga gcctcaacgt ccgtgcaggt ttactgacgg tgactcaatt ttacacaatg
    14821 aattttattg tttaagaacc ttaaaaacat agtgagtttt taaagactta ataataagca
    14881 tatacggtcc agccggtaaa tgttcaataa ataaattggc ttctaaatca ttattcacga
    14941 ccaggaagtc ctttctgtcc atttcctgcc ccatggcatt gaatagtaat atctggtatt
    15001 tattgtcctt caagcctgaa ccctttattg taagaaagcc actggttgga tttgggaata
    15061 cctccattgt cgaacctcct tccgattcag atacgcttgt gatagttacc tcgtggcaac
    15121 cagatacaat tgaacatcca ttcagtgtga caactacagc ataatttcca ttttgcatcg
    15181 gagtaaatga gcgcccgttt tcacctagaa tggaatccaa tccagggcaa ttaacccatt
    15241 gataagaggc tgatgccgca ttagcggtca acgttatccc atttacgttg ataaaagtgt
    15301 caattacagt tgttgaaacg gatatagttt gtgcagctcc tacaccgcat gagtttatag
    15361 ccgatacaga aaatataccg ccaaaagtac ccgcagtagt agtaatcgaa ttggttgaag
    15421 aattgcccga ccagccgaaa ggtagcgtcc agtaatagct cgttgcttct gggattggcg
    15481 caatagaata tatttgtgtc gagccttgac atatactact cgtgttacct gatatcggcc
    15541 ccggttgcga aggagtgggt gttacgttga ctgttaatgt gctgggggaa ctattaccac
    15601 aactgacatt ggccgtgaca aaaattgctc cgcccgaact acccgcagta gctgagatgg
    15661 atgtggacgt cgaggttcca gaccagcccg atggcaaggt ccaggtataa ctcgtcgcac
    15721 cgactactgc gctgatggaa tatgtctgcg ccgaaccctg gcatacgctg gtgttcccag
    15781 agatagcgcc gggctgcaca ggtgtcggtg atacgttgac attcaaaacg ctgggcgaac
    15841 tcgcgccgca ggcattat
//
LOCUS       DAORLF010000045        15483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2048_length_15483_cov_1.976471, whole genome shotgun sequence.
ACCESSION   DAORLF010000045 DAORLF010000000
VERSION     DAORLF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_15483_cov_1.976471"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="PK198_06055"
     CDS             <1..1355
                     /locus_tag="PK198_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38328.1"
                     /translation="DGTAGTVHVIFIDAWDAAGNRDFCETYVRVQDQSGICTGPALGS
                     IAGTIRTETQQTVEGVEVALSGPMTANATTPTAGTYSFSNLEENHDYTITPVLDVNYL
                     NGVSTFDLVLIQRHILTVQPLNSPYKMIAADANNSQTITTSDIIILRRLILSIIDDLP
                     NNTSWRFVPTSYTFPVPTNPWFEQFPEVINENDLQGDVLGRDFVAVKIGDVNGTVVPN
                     LASVEDRNVQGTFGFDLEERAVKAGEEVKVAFRGKDMERIMGYQLTLGFDRTALSLDN
                     IEYGVAKEGNFGLRYVSEGMITMSWHEMNAGNRIKSDEVLFTLVFRATASGKLSDLLS
                     VSSRLTRAEAYDRNSRTLDVSLNFGSRELTEEYALYQNQPNPFAGKTTIGFYLPNAGE
                     ATLTITDVRGRVLKVFKGEYGRGMHQIQLDGEDLPAGVLQYTLISGDFTATRKMVLTR
                     "
     assembly_gap    1452..1530
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1531..2005
                     /locus_tag="PK198_06060"
     CDS             <1531..2005
                     /locus_tag="PK198_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38329.1"
                     /translation="LDILPVCTSQVLFIKNDTMNKRSIVYRRGAAQWPGHFSEESAFI
                     RADVVLGSPKSKCSGVGICRISASGSGKAYPCPVYSVEVIGCGGNQMKLIFEKAHLEP
                     SIIEKHFKGGYFCVEEDYIIPRDLVERLKMKKRWIRPGVYPVQQTAAHIMVIFTL"
     gene            2250..3269
                     /gene="galE"
                     /locus_tag="PK198_06065"
     CDS             2250..3269
                     /gene="galE"
                     /locus_tag="PK198_06065"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRF38330.1"
                     /translation="MPKILVTGGCGYIGSHTIVDLIEHGFEVVSVDNFCNADASVLDG
                     MEAITGRRVFNYATDLCYLPATRLIFEEHPDIAGVIHFAALKAVGESVEQPLRYFHNN
                     LNSLLHILTCMRDFGVRHLIFSSSCSVYGNAAELPVTETTPWQEAESPYGRSKQMGEQ
                     IIRDALPACPGMNSILLRYFNPAGAHPSALIGESPSNPASNLVPVITETAMGLRREMT
                     VFGADYPTRDGSCVRDYIHVMDLANAHTKALQYLLEQRNESPCEVFNLGMGQGVTVLE
                     ALAAFERVSGVQPAYQIGLRRPGDVVAVYADNARAVERLGWQPGYRIDDIMRSAWDWE
                     QRRRG"
     gene            3351..>4219
                     /locus_tag="PK198_06070"
     CDS             3351..>4219
                     /locus_tag="PK198_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38331.1"
                     /translation="MARYIFLLSVVLFNNHCFAQREFGVCDSTSYALNADTTIIAGRH
                     HLYLYSNNTLSTLYDFTSPDDREYIRDFDIIHPDLWYTVVGSRYIGGPTRLYKSTNGG
                     QIWTLDTSHHNAANSGFLSPQFFQSINNLQHLNGDTLMMFMSYYESGIIYSTDLGLTW
                     TKWFHNLISYYQGMFECNDKYYIFGYQGDGFRASMFSFDKNLLFTSDSTGLWNSFNHL
                     GYHPPCYNGADLERCIFAPHNSTRCESYYYFENKIDTLCSALSIEDVNTIVYRVFPNP
                     FHEKITVEGDTGT"
     assembly_gap    4220..4234
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4413..5918
                     /locus_tag="PK198_06075"
     CDS             4413..5918
                     /locus_tag="PK198_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRF38332.1"
                     /translation="MKILLVYPEMPDSFYAFKHLTSLVGKKAAFPPLGLLTVASMLPV
                     NWERRLVDLNVKPLLDKDIKWADLVFLSAMNVQEESVREVIAQCRKNNTKIVAGGPLF
                     THEHDRFEGIDYFVLNEAEITLPLFLADLEMGKPQPIYQTKEFADISKSPLPQMELID
                     MSDYLYAIVQYSRGCPYLCDFCDVTTLFGRKPRTKSPEHIIAELEVIQKAGNTQMVLF
                     ADDNLIGNKKILKSELLPALIEWRKKRSPGFYFATQLTVNLADDDELMHLLLEAKFGN
                     VFIGIETHQEDSLKLSQKNQNLRRNLLDTIHKIHKAGFIIAGGFIVGFDTDTDKSFDE
                     MIAFIQESGIPLPIVNVLKAPPGTALFNRMKLENRLSQHFTFSEGETNVIPVMGEEKL
                     TNGFLHVVSNIYLPEHAHKRIITFLETHGIHKSSTKVAVKYSVSQLLHLSKAVIKLAV
                     FDKDRKYFWKLLLWAYRHKRNHLDLAVLYGMMLYQMRENYRQIISRLVKAA"
     gene            5976..6473
                     /locus_tag="PK198_06080"
     CDS             5976..6473
                     /locus_tag="PK198_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRF38333.1"
                     /translation="MMQQDLQAWKEQAQRDRKDTGAYFKRLRRKPPKQLDEVMETLHE
                     EVFEQTDCLACANCCKTTSPIFRDKDIERIARHLGMRPATLVQQYLHIDEDGDYVLNE
                     SPCAFLGADNYCSIYDVRPKACRDYPHTDRKQFHQILDLTLKNTQVCPATYEIIKRLK
                     AALPM"
     gene            complement(6474..7517)
                     /locus_tag="PK198_06085"
     CDS             complement(6474..7517)
                     /locus_tag="PK198_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRF38334.1"
                     /translation="MPRLYNLVNNEELKRQMLETDEKRITFSFYQYWNLPDPQAFRDS
                     AYRQWSQLGVWGRVYVAQEGINAQVSVPESQFEAFKNALYSVPQLNGIRLNIAVEDNG
                     KSFYKLKIKVRPKIVADGLDDSSFDATRRGQGLNARDFNALTDDPGAIVVDMRNHYES
                     EVGHFENAICPDVVTFREALPLVEEMLHDKKDRPVVMYCTGGIRCEKASAYFLHKGFQ
                     NVYMVEGGIIEYARQCADQGLPNKFIGKNFVFDERLGERISNDVIAQCHQCGASCDDH
                     TNCANDACHLLFIQCPACAARYAGCCSTHCADFAALPETERERLKPTMTFNGTKFTKG
                     RYKSYRRGEEELG"
     gene            complement(7674..>9292)
                     /locus_tag="PK198_06090"
     CDS             complement(7674..>9292)
                     /locus_tag="PK198_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38335.1"
                     /translation="DMAANTAAQLEATCNATPPAASGTFQPVNPLATFAGEPASGVWT
                     MRIQDAASSDGGSLQAWSIEICTALPHEVSLYSAQSQFTLCASDTARIEFIVGTAFTN
                     PVNLTMTGLPAGTQYAFSPQPAAAGQTVTAIVWGFTSSGNITASLQAEAGAQTAAAPI
                     NFTVAPPPAAPALLAPTPGAVNVALNPTLTWGAVTGATSYRLWVATDTEFTNIVVNPT
                     LSATSFNLNNLLNGTTYYWRVATQGACGWGQPGARQQFTTVPDLSMSASPMSVSICNT
                     GQAVYTINLGPGFVPPLTFTFSSSGPSPVLPQLSFATNNGVHTASVTNEIQLLRGPYQ
                     LVITVADAAGNTNNTAALLIIETTPPFPTLSAPANNAVVMTPTPMMSWNTATGADEYR
                     VEVARDETMMDIVATQVQTGTSWTPSQALVAGDYFWRVTAQNECGNATTAPFKFTVTP
                     SAIHEVQGQRLMIDPNPTQGPVFVRLQHQASADWVASVYDLNGILVQKRVIARGETSA
                     VLDLSAYPAGMYVLRLQSGEAVVTQQIVKQ"
     assembly_gap    9293..9302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9303..11496)
                     /locus_tag="PK198_06095"
     CDS             complement(<9303..11496)
                     /locus_tag="PK198_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF38336.1"
                     /translation="MHIKTIIAAILLLWATIAPAQSIAKLKTPLSEVPLIEMPRLDNE
                     TLRAEEMSQRAPGRAPRFAQRHPVSISPQTHGRWETTPSGIAVWRLRLRSAGALSLNL
                     GFDQYVMPEGGHLFLFSPDQRNQLGPFTPSDNQAHAQLWTPVLNGDEMVIEVQVPVAK
                     RNELQLHLESVNHDFLGFSQMLSGSCNLDVICGAADGWAIVDRYRDIIQSVGVYGLGG
                     ETFCTGFLINNARNDCAPLFMTANHCGVRDNNAATLVVYWNFQNSTCRQPNTPASGQP
                     GNGQLNLFNTGSVLLARNTTSDFCLVELNNDVPAAADAFYAGWNISDQAPQDSVICIH
                     HPSTDEKRISFEFNPTVIGGTNGAANPSGNFIVVPDWDTGTTEGGSSGSPLFNRRKQV
                     VGQLQGGAAACGNDSYDAFGWIYRSWTGGGATTNSLRPWLDPDNTGITEMDGRSARSC
                     SFSIEALAANVELCAPQTAQYQLTVSENFTGPVTLSLSGTPAGAAAAFSVNPVMPGAA
                     TMLTISNTGGVAPGSYAMSVSGTDGTNTDMAALNLLINVGAPVAPVLQSPANAAIGAS
                     IAPTFTWQAQQAGTQYEFQIASDAAFNNIIAQNTGISANSTGGIALQPNTTYYWRVRG
                     NNLCGEGPWSAAYSLTTALTLCAAPLAGTGLPITISAQGTPTINSTVTVSAPGTVASV
                     KINGLDIRHTWVGDVRATLISPAGTSVVLFDQIGFPASSFGCDGDNLLL"
     assembly_gap    11747..11776
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11777..>12030)
                     /locus_tag="PK198_06100"
     CDS             complement(<11777..>12030)
                     /locus_tag="PK198_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF38337.1"
                     /translation="RLMALAGAEMLIYPTAIGWESTDATAEKERQFGAWQTIQRGHAV
                     ANGLPVITVNRTGHEPDWSGVTNGIEFWGQSFVAGPQGEL"
     assembly_gap    12031..12099
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12100..12528)
                     /locus_tag="PK198_06105"
     CDS             complement(<12100..12528)
                     /locus_tag="PK198_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF38338.1"
                     /translation="MTTSSHTTIKTGIVQQSCSRDTEANIQKSLAGIRACAEAGAQLV
                     VLQELHAGVYFCQAEDVAQFDLAEPIPGPISERFAAAAREYGVVLVTSLFEKRAPGLY
                     HNTSVVFEKDGSTAGKYRKMHIPDDPGYYEKFYFTPGDLGF"
     gene            complement(12645..>14045)
                     /locus_tag="PK198_06110"
     CDS             complement(12645..>14045)
                     /locus_tag="PK198_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38339.1"
                     /translation="RNHVINRATVNVRGKRNYQAEGYYEYNIGTRGQEIHFQNIVGQP
                     VGRGKISERPSVTRATGEVAADKDFYIDHKTKFKGTISLNAESKALKFEGFALLDADK
                     LPYPEWFSVSSEADKNDLIIRYATPKNENGDPLYTGLYLGKESANIYPAVMQPLTFRK
                     DRDIFPAAGFFKYIKEKDQFIFGDSTRVVASQVRGNLFTFKNSDGSVEADGRFNIGSA
                     LKYVKVDAAGFAKGKFPPPPAGDVMLDEEPDATTKNQVDPLRAELMTGITMTLPDALA
                     KVMVTDFRSSTFDAAPIVYLTDLDYYRRAATQLLPDDKDAREAIGGLSSGYLDLPKRI
                     NPYTFLFSRLKLKWDVEYQSFIGTEQNIGLVSIAGESINRVVTGYIEFKMPSNDDDRL
                     YIYLKSPSEQFYFFGFKQGILNVTSNNPNFMSALTAMKAKDRIIKMPDGANYEIQPLE
                     ESEAQRFVRRIQAAKK"
     assembly_gap    14046..14183
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14184..>15483)
                     /locus_tag="PK198_06115"
     CDS             complement(<14184..>15483)
                     /locus_tag="PK198_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38340.1"
                     /translation="FSTRQKVALKPDSSEITLRADRNMDFNGRLFAGFTVLEGKKFRF
                     DYNKFLVHLDSVRYFDIFLPTGEFDKNRNPVAFGIQSRIEYLTGALLIDAPGNKSGRD
                     NIVLFPSLESKSKSFVFYDRPETQQGVYVRDSFYFELAPFSFQHLDVLAAKDLVFKGK
                     LLSSDIFPPFAESISLQKDQSLGFISNTPDKGLPAYRGKGNYSGAIELSNQGLLGKGN
                     LSYLGAQLNAKDIVFKPSEMLAKADIFDMKESRSGAVQTPQVHGDSVSILWRPYRDSM
                     YISSETAPFAIFQSGKHTVAGTLILTPGGLKGNGLLEWDKAGMRSKLLSFGANSVKAD
                     TSNIDIRTIGVEGVAIRTKNVNSDVDFDKKIGKFKANTPGEWTDFPYNQYQTSLNEYT
                     WNMDEETIVFKSEKGIKGKFRSVHPEQDGLNFEGETAYYDL"
BASE COUNT         3445 a         3597 c         4455 g         3645 t
ORIGIN      
        1 cagacggtac ggcaggcaca gttcatgtaa ttttcataga cgcatgggat gcagccggca
       61 accgcgactt ctgcgagacg tatgtacgcg tacaggatca gagcggtatc tgtaccggcc
      121 cggccttggg ttccattgcc ggtacgatcc gcacagagac gcaacagacg gtagaaggcg
      181 tagaggtagc gttgagcggc ccgatgacgg ccaatgcaac gacgccgacc gcgggtacct
      241 atagcttcag caacttagag gagaaccatg actacaccat cacaccggtg ttggatgtca
      301 attacctcaa tggtgtctct accttcgact tggtgttgat tcagcgccat atcctgacgg
      361 tgcaaccgtt gaacagcccg tacaagatga ttgcagcgga tgccaacaac tcgcagacga
      421 tcaccacgag cgacatcatc atcctgcgcc gattgattct gagcatcata gacgatctgc
      481 cgaacaacac cagttggaga ttcgtaccga cgagctacac gttcccggta ccgaccaacc
      541 cgtggttcga gcagttcccg gaagtcatca atgagaacga cctgcaaggc gacgtactgg
      601 gccgcgactt cgtagccgtg aagatcggcg atgtaaacgg caccgtggtt ccgaatctgg
      661 cttccgttga agaccgcaac gtacaaggta ccttcggatt cgatttggaa gagcgcgcag
      721 tgaaagccgg tgaggaagtg aaagtagcct tccgcggcaa agacatggag cgcatcatgg
      781 gctaccagtt gacgctgggc tttgatcgca cggcactgag cttggacaac atcgaatacg
      841 gcgtggcgaa agaaggcaac ttcggtttgc gctatgtaag cgagggcatg attacgatga
      901 gctggcacga aatgaatgcc ggcaaccgga tcaagtccga cgaggtgctg ttcacccttg
      961 tgttccgcgc cacggcctcc ggtaagctca gcgacctgtt gagtgtaagc agccgcctga
     1021 ccagagccga ggcttacgac cgcaacagcc gcacattgga tgtatcgctg aacttcggca
     1081 gccgcgagtt gacggaggag tatgcgctgt accagaacca gcccaaccca tttgcgggca
     1141 agacgacgat cgggttctac ctgcccaatg ccggcgaggc tacgctgacc atcacggatg
     1201 tgagaggccg cgtgctcaaa gtcttcaagg gcgagtacgg acgtggaatg caccagattc
     1261 agttggatgg agaagacctg ccggcaggcg tgctgcaata cacgctgatc agcggagact
     1321 tcaccgcaac tcgtaaaatg gtgttgacga gataagggtt aagtgtcaaa aagaacaacc
     1381 tgaatagttg ttcggacaga taaagtgtaa caggggcgta gggcaattag tgtctgcgcc
     1441 cctgttttat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttggacatt ttgccggtat gtacttccca
     1561 agtacttttt attaagaacg acactatgaa taaaaggagc attgtgtacc gtcggggcgc
     1621 tgcgcagtgg ccggggcatt tttcagaaga gtccgcattc atcagggcag atgtggtatt
     1681 gggttcccca aaatcaaaat gctcgggagt gggtatttgc cggatatcgg catctgggag
     1741 cggaaaggca tatccgtgtc cggtatattc cgttgaggtc attggttgtg gcggcaatca
     1801 aatgaaactc atttttgaga aggcgcactt ggagccatcc ataattgaaa agcattttaa
     1861 gggaggttat ttttgtgtgg aggaagatta catcattccc cgtgacctgg tcgaaaggtt
     1921 gaagatgaag aagcgatgga ttcggccggg ggtgtatccg gttcagcaga ccgctgcaca
     1981 tataatggtg atattcacat tataagaatg aaattggagg aatcatggtt cggttggcgg
     2041 taatgattgg aaaggaaaga aactaaatcc aaaacaaaga tgtaaccttg taaacaaaga
     2101 tgagacaggc tggaaagcca atcataattt tcatgtgatt atgatttgtg taggcggtcg
     2161 cttcaaaaga gcggccactt tttttgtata tatggcgctg gactttgatt ttctctccaa
     2221 ctttgtcccc tcaacaacac aaaacaacca tgcccaaaat cctcgtcacc ggaggttgcg
     2281 gttacatcgg cagccatacc atcgtggacc tcatcgaaca cggcttcgag gtcgtcagcg
     2341 ttgataactt ttgcaacgca gatgcctcgg tgttggacgg catggaggcc atcaccggcc
     2401 ggagggtatt caactatgcc accgacctct gctatctgcc cgctacccgc ctcatttttg
     2461 aagagcatcc cgacatagct ggagtcatcc attttgcggc gctcaaggca gtgggggagt
     2521 cggtggagca gccgctgcgg tactttcaca acaacctcaa cagcctgctc catatcctca
     2581 cctgtatgcg ggattttggg gtgcggcatt tgattttttc atcttcctgc tctgtgtatg
     2641 gaaatgctgc cgagttgccg gtgaccgaaa ctacgccctg gcaggaggcg gagtcgcctt
     2701 atggtcggag caaacaaatg ggcgagcaga tcatccgcga cgcgctgccg gcttgtccgg
     2761 gcatgaacag tattttgttg cgatacttca acccggcggg ggcgcatccc tcggcgctca
     2821 tcggcgaatc accgtccaat ccggcgtcca atctggtgcc ggtcattacg gaaacggcga
     2881 tgggattgcg gcgtgaaatg acggttttcg gtgcggatta tcctacccgc gacggcagtt
     2941 gtgtgcgcga ttacatccat gtgatggacc tggcaaacgc gcataccaaa gctttgcaat
     3001 acctgttgga gcagcgcaat gagtcgcctt gcgaggtgtt caatctcggc atgggtcagg
     3061 gcgtcacggt gctggaagcc cttgctgcgt ttgagcgggt ttcgggcgtg cagccggctt
     3121 accaaatcgg gctgcggcgc ccgggcgacg tggtggcagt gtatgccgat aatgcccgtg
     3181 ccgtagagcg tttgggctgg cagccggggt accgtataga cgacatcatg cggagcgcct
     3241 gggattggga acagcgccgg cggggctgat cggcagtctg tttgtatcgg cgtatggtct
     3301 tctttttcag gaaattaaca agtggtatgt aacttgaaaa cacctttctc atggccagat
     3361 atatcttctt actttcggtt gttttattta acaatcactg ttttgcacag cgcgagtttg
     3421 gagtttgcga ctctacttct tacgcactca atgcagatac gaccattatt gcaggcaggc
     3481 atcatttgta cttgtattct aataatacgt tgagtacgtt atatgatttc acatcgccgg
     3541 atgacagaga gtacatcaga gattttgata tcattcatcc cgatctttgg tacacggttg
     3601 tagggagcag atacatcgga gggccgacaa ggttatataa aagtaccaat ggaggccaaa
     3661 tctggacgct ggatacgagc caccataatg ctgccaactc agggtttcta tctccgcaat
     3721 tttttcaatc catcaataat ttacagcatt tgaatggcga tacattgatg atgtttatga
     3781 gctattacga gtcggggata atctattcta ctgatttggg gctgacctgg accaagtggt
     3841 ttcacaattt aatatcatat tatcagggca tgtttgaatg caacgacaag tattatattt
     3901 ttggatatca gggtgatgga ttcagggcct ctatgtttag ttttgataaa aacttattgt
     3961 tcacatcaga cagcaccggg ctttggaatt cgttcaatca tcttggttat cacccaccct
     4021 gttacaatgg ggccgacttg gaacgttgta tttttgcgcc gcacaattcg acccgctgcg
     4081 aaagttatta ttattttgaa aataagatag ataccctttg ttctgcgctg agtatagagg
     4141 atgtaaatac cattgtttac agggtttttc ccaatccgtt tcatgagaaa ataaccgttg
     4201 aaggcgatac ggggacggan nnnnnnnnnn nnnnccctca taaatgttta tggacaaacg
     4261 gcttggtcgg gccggttgat agcgcaacag gattttttcc ggttgacaaa tggagtttat
     4321 tttttgacga tttcaaaggg agattttagt cagactatac aacttattaa atattaaatg
     4381 ctccagctat gcaacaagac tgtttcgata ccatgaaaat tctgctggtt tatcctgaaa
     4441 tgcccgattc gttttatgcc tttaagcacc tcacttcgtt ggtgggtaaa aaagcagcgt
     4501 ttcctccatt gggattgctt acagttgctt ctatgttgcc ggtaaattgg gagcgcaggt
     4561 tggttgattt aaatgtaaaa cctttattgg ataaggatat taaatgggct gacctggttt
     4621 ttctttcggc catgaatgtg caggaagaat ctgtgcggga ggttatcgcg caatgcagga
     4681 aaaataacac caaaattgtt gcaggcggcc cactgtttac acatgagcat gatcgttttg
     4741 aaggaataga ttattttgta ttaaacgaag cagaaattac ccttcctcta tttttagctg
     4801 atttggaaat gggcaagcca caacccattt accaaaccaa agagtttgcg gatatcagta
     4861 aaagccccct tccgcagatg gagttgattg atatgagtga ttacttgtat gccattgtac
     4921 aatactcacg cggatgtccg tatttatgcg atttttgtga tgtgacgacg ttatttgggc
     4981 gcaagcctcg caccaaatcg cctgaacata ttattgcgga attggaggtg atacaaaaag
     5041 ccggtaatac gcaaatggtg ctgtttgctg acgataattt gattggcaat aaaaaaattc
     5101 taaaatctga gttactgcct gcgctgattg agtggcgcaa aaaacgctct ccgggttttt
     5161 attttgccac ccaacttacc gtaaatttgg ctgatgatga cgaattgatg catcttttat
     5221 tagaggccaa gtttggcaat gtgtttattg gtattgaaac ccatcaggaa gacagtttaa
     5281 agctttcgca aaaaaaccaa aacctgaggc gcaacctgct ggataccatt cataaaatac
     5341 acaaggccgg gtttattatt gccggaggct ttattgtagg ttttgataca gataccgata
     5401 aaagttttga tgaaatgatt gcttttattc aagagagtgg aatcccgctg cctattgtaa
     5461 atgtactcaa ggctcctccg ggcactgcgt tatttaacag gatgaagctt gaaaacaggc
     5521 tttcgcagca ttttactttt tcggagggcg aaaccaatgt gatacctgta atgggtgaag
     5581 aaaaattaac caatggtttt ttgcatgtgg tttctaatat ttatttaccc gaacatgcac
     5641 ataaaagaat tattaccttt ttagaaaccc acgggattca taaatcttct acgaaggttg
     5701 ctgtaaaata ttcggtttcc caactcttac atttatctaa agcggtgatt aaacttgccg
     5761 tatttgataa ggaccggaag tacttttgga agttattgct ttgggcgtac aggcacaaac
     5821 gcaaccactt ggatttggcg gtgctgtatg gaatgatgtt gtaccaaatg agggaaaatt
     5881 acaggcaaat tatcagcaga cttgtcaaag cagcttaata gttagagacg gttcatttaa
     5941 gtgtgtccac tatctttgcc ccgcaaatta ctgtgatgat gcaacaagac ttacaggcat
     6001 ggaaggagca ggcccaacgc gaccgcaaag ataccggcgc ctacttcaaa cgcttgcgcc
     6061 gaaaaccgcc caagcaattg gacgaggtga tggaaacgct gcacgaggag gtttttgaac
     6121 agacggattg cctcgcttgt gccaattgtt gcaaaaccac cagccccatt tttcgggata
     6181 aagacataga acgcattgcc cggcacctcg gcatgaggcc cgcaaccttg gtgcaacaat
     6241 acctgcatat agacgaagac ggggattatg tactcaatga atcaccctgc gcgtttttgg
     6301 gcgccgacaa ctactgctcc atttacgatg tgcgccccaa agcctgccga gactacccgc
     6361 ataccgaccg caaacagttt catcagatat tagacctcac cctgaaaaat acgcaggtgt
     6421 gcccggctac ttacgaaatc atcaaacggc tgaaggctgc cctgccgatg taatcacccc
     6481 aactcctcct cgccccggcg atacgatttg tacctgcctt tggtaaactt cgtgccgttg
     6541 aacgtcattg tcggtttcag gcgctcgcgt tccgtttcgg gcagcgcggc aaaatcggcg
     6601 cagtgcgtag agcaacaacc cgcataccgg gcggcgcagg cggggcactg tatgaagagc
     6661 aggtggcagg cgtcgttggc gcagttggta tggtcgtcgc aggacgcgcc gcactggtgg
     6721 cattgggcaa tgacgtcgtt ggaaatgcgt tcgccgaggc gctcatcgaa gacaaagttt
     6781 ttgccgatga atttattggg caaaccctgg tctgcgcatt gccgggcata ctctatgatg
     6841 ccgccctcca ccatatacac attttgaaaa cccttgtgca ggaagtaagc cgacgctttt
     6901 tcgcagcgaa tgccgccggt gcagtacatc accacggggc ggtctttttt gtcgtgcagc
     6961 atctcctcca ccaaagggag cgcctcgcgg aaggtgacca catcggggca gatggcattt
     7021 tcaaaatggc ccacctcgct ttcgtagtgg ttgcgcatgt ccaccacgat ggcgccggga
     7081 tcatcggtga gggcgttgaa atcgcgggca ttgagacctt ggccgcggcg ggtagcatcg
     7141 aagctgctgt cgtccaatcc gtcggctacg attttgggcc gcactttgat tttgagtttg
     7201 taaaacgact ttccattgtc ctctacggct atgttgaggc ggatgccgtt gagttgcggc
     7261 acgctgtaca gggcgttttt gaaggcttca aattgggatt ccggcaccga cacctgcgcg
     7321 ttgataccct cctgtgcgac atacacgcgg ccccaaacgc cgagctgcga ccattggcgg
     7381 taggcgctgt cgcggaatgc ctgcgggtcg ggcaggttcc aatattgata aaaagaaaaa
     7441 gtgatgcgct tctcgtcggt ttcgagcatt tggcgcttca gttcctcgtt attgaccaag
     7501 ttgtacagtc tgggcatgtg aatgttttgg cagtaaatcg ggtatgataa aattcaatcg
     7561 ggctgcaaag gtagggaatt acataaaaaa acggcaagcc gggagcacct gtcccaacct
     7621 gccgttttta gggtaaaata actcgtagct cgcagctaaa agctcgcggc tccctactgc
     7681 ttcacaatct gctgcgtcac aacggcctcg ccgctttgca acctcagcac atacatgcct
     7741 gccgggtacg ccgacaagtc gagcacggca gaagtctcgc ctcttgcgat gactcgtttt
     7801 tgtaccaaaa taccgttgag atcatacaca gacgccaccc aatcggcgga agcctgatgt
     7861 tgcaatctca caaaaaccgg cccctgtgtc gggttgggat caatcatcaa tcgctggccc
     7921 tgcacctcgt gaatggcgct cggcgtcacc gtaaatttga aaggcgcggt agtggcgttg
     7981 ccgcactcgt tttgagccgt cacccgccag aaataatcac ctgcgaccaa tgcctgagac
     8041 ggcgtccagg aagtgccggt ttgtacctgt gtggccacaa tatccatcat ggtttcatcg
     8101 cgagccacct ctacccggta ttcgtcggcg ccggtagccg tattccaact catcatcggc
     8161 gtgggcgtca ttacaacggc gttgttggcc ggcgctgaga gggtggggaa gggcggcgtt
     8221 gtttctataa tgagcaaagc ggctgtgttg ttggtgttgc ccgctgcatc ggcaaccgta
     8281 atgaccaact gataggggcc gcgcagcaac tgaatttcat tggttaccga agcggtatgc
     8341 acgccgttat tggttgcaaa actcaactgc ggcagcacag ggcttgggcc ggaagaggag
     8401 aatgtaaacg tgagcggcgg aacaaagccc ggccccaagt tgatggtata aacagcctgc
     8461 ccggtattgc aaatgctgac gctcatcggg gaagcgctca tgctcaaatc gggtacggtg
     8521 gtaaactgct gccgggcgcc gggttggccc caaccgcatg cgccttgagt agccacccgc
     8581 cagtagtagg tggttccgtt gagcaaattg ttgaggttga acgaagtagc gctcaatgta
     8641 ggattgacaa cgatgttggt aaactccgta tctgtggcca cccaaagccg gtaggaagtc
     8701 gctcctgtaa ctgcgcccca ggtcagggtc gggttcagcg ctacattcac tgcgccgggg
     8761 gtgggcgcaa gcagtgccgg cgcggcaggc ggtggagcga cggtaaagtt gatcggtgca
     8821 gcggccgttt gcgctccggc ttcagcttgc agggaggccg tgatattgcc gctggaagtg
     8881 aagccccaca cgatagccgt cacagtttgg ccggctgcgg cgggttgagg ggagaacgca
     8941 tattgtgtgc cggcgggcag gcccgtcatc gtcaggttga cggggttggt gaaggccgtg
     9001 cctacgatga actcaatacg cgccgtatcg gaagcgcaga gcgtaaactg cgattgtgcc
     9061 gagtacaaac tgacctcatg cggcagggca gtgcatattt cgatgctcca tgcctgcaaa
     9121 gaaccaccgt cgctgctggc cgcgtcttga atacgcatgg tccaaacccc tgatgccggc
     9181 tctccggcaa aagtcgccag cggattgacc ggctggaagg tgccggatgc tgccggcgga
     9241 gttgcattgc aggtggcttc caattgagca gccgtgttgg cagccatatc gtnnnnnnnn
     9301 nnagagcaga aggttgtcgc cgtcgcagcc gaatgatgag gccggaaatc ctatctggtc
     9361 gaacaaaaca acagaagtac ctgccggcga aatcagcgtg gcgcgcacgt cgcctaccca
     9421 ggtatggcga atgtccaagc cgttgatttt tacagaagct accgtgcccg gcgctgaaac
     9481 ggtcacggta gaattgatgg tgggcgtacc ctgagccgag atggtgatgg gaagacctgt
     9541 acccgcaaga ggcgcggcgc acaaggtcag tgccgtcgtc agggagtagg ctgccgacca
     9601 ggggccttcg ccgcagaggt tgttgccgcg tactcgccag tagtaggtgg tattgggttg
     9661 cagggcaatg ccgccggtac tgttggcgct gatgccggta ttttgggcaa tgatgttgtt
     9721 gaaagctgca tctgaggcta tttggaactc atactgcgta cctgcttgct gtgcttgcca
     9781 ggtgaatgtg ggcgctatgg atgcgccgat ggcggcattg gcaggagact gcaacacagg
     9841 agccaccggt gcgcccacat tgatgagcag gttgagcgcg gccatatctg tgttcgtacc
     9901 gtcggtgccg gaaacactca tcgcgtagct gccgggagca acgccgccgg tattggaaat
     9961 ggtgagcatg gtggcagcgc cgggcatcac gggattgacg gaaaaagccg ccgcggcacc
    10021 ggcaggagtg cctgaaagcg aaagcgtgac ggggccggtg aagttttcac ttaccgtcaa
    10081 ttgatactga gccgtttgcg gagcgcaaag ctctacattg gccgccaaag cttctatgct
    10141 gaaagagcag gatcgcgctg agcgcccgtc catttctgtg atgccggtat tgtcggggtc
    10201 gagccagggg cgcagactgt tggtggtggc tccaccgccg gtccaggagc ggtatatcca
    10261 gccaaatgcg tcgtaagagt cattgccgca ggctgcagcg ccgccctgta attggcccac
    10321 cacttgcttt cttctgttga acaaaggcga gccggaagaa ccgccctcgg tagtgccggt
    10381 gtcccagtcg ggtaccacga tgaagttgcc gctggggttg gcagcgccgt tggtgccgcc
    10441 aatgacggta ggattgaatt caaagctgat gcgcttttcg tctgtactgg ggtggtgaat
    10501 acagatgacg gagtcttgcg gcgcctggtc ggagatgttc caacctgcgt aaaaagcatc
    10561 ggcggctgcc ggcacgtcgt tgttcaattc taccagacag aagtcggagg tggtgttccg
    10621 ggcgagcaag acagaacctg tattgaagag gttgagctgg ccgttgccgg gctggccgct
    10681 ggcgggggta ttgggttgac ggcaggtgct gttttggaaa ttccaatata ccaccaatgt
    10741 ggcggcattg ttgtcgcgga cgccgcagtg gttggcagtc atgaacaggg gggcgcagtc
    10801 gttgcgggca ttgttgatca aaaagccggt gcaaaaagtt tcgccgccga ggccatacac
    10861 gcctaccgac tgaatgatgt cgcggtagcg gtccacgatg gcccagccgt cggcggcgcc
    10921 gcagatgacg tccaaattgc aggagccgga gagcatttgc gaaaagccta ggaagtcgtg
    10981 gtttaccgat tcgaggtgca attgcagttc gttgcgcttg gctacgggta cttgtacttc
    11041 aatgaccatt tcgtcgccgt tcagtacggg cgtccagagt tgagcatgcg cctgattgtc
    11101 ggaaggggtg aacgggccga gttgattcct ctggtcgggg gagaacaaaa acaggtggcc
    11161 accttcgggc atgacgtact ggtcgaagcc gaggttgagc gacagggcgc cggcagagcg
    11221 caggcggagc ctccatacag ctatgccgga gggcgtggtt tcccaacggc catgcgtctg
    11281 gggagagatg ctgacagggt ggcgttgtgc aaaacgcggg gcacggccgg gcgcgcgttg
    11341 agacatttct tcggcgcgga gggtctcatt gtccaatctg ggcatctcaa tcagcggtac
    11401 ttcactgagc ggtgttttta gtttggcaat ggattgggca ggtgcgatgg ttgcccatag
    11461 cagcaggatg gctgcgatga tggttttgat gtgcatatac aggcgttgtg agtgaatgtg
    11521 actattacat gtaaaacagt agcaaatcta taccatagta tcggggtagc caaacgaatg
    11581 gggtgcaatg tcgtgtaaag ggcaaaaatg ggtcttactg ctcgtctaaa tacctttggg
    11641 taaggccctg gtaggcgtct atgcggcggt cgcggaagaa gggccagatg cggcgcacgg
    11701 cctcggtgcg ttgtttgtca atgtcaatga cggcgtggca ctcgtcnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnngagt tcgccctgcg gcccggctac aaagctctgg ccccagaact
    11821 cgatgccgtt ggtgacgccc gaccaatcgg gttcgtggcc ggtgcggttg acggtgatga
    11881 ccggcaggcc gttggcgact gcgtggccgc gttgaatggt ttgccaggct ccgaattgac
    11941 gttccttttc ggcggtcgcg tcggtggatt cccagccgat ggcggtgggg tagatgagca
    12001 tttcggcgcc ggccagagcc atgaggcggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aagccgagat cgccgggggt
    12121 gaagtagaat ttttcataat aaccgggatc gtcggggatg tgcatcttgc ggtacttgcc
    12181 ggcagtggag ccgtcttttt caaacacgac ggaggtgttg tgatagaggc cgggcgcgcg
    12241 cttttcgaac agggaggtga cgagcacgac gccgtattcg cgggcggcgg cagcaaaacg
    12301 ttcggaaatg gggccgggaa tgggttcggc gagatcaaac tgcgccacat cttcggcttg
    12361 gcagaaatag acgcctgcgt ggagttcctg caaaacgacg agctgcgcgc cggcttcggc
    12421 acaggcgcgg atgccggcga ggcttttttg gatgttggct tcggtgtcgc gtgagcagga
    12481 ttgctgtacg atgccggttt tgatggtggt gtgggacgat gttgtcattt tgttggatag
    12541 aatgaagaaa cgaaaaaccg caaaataata aatgcaaaat gtaaaagtgg ctgcgcgaga
    12601 ggcgttttac attttacatt cctctgtttt acattttaca tttcttactt cttcgccgcc
    12661 tgaatgcgcc gcacaaagcg ctgggcttcg ctctcttcca gcggctgaat ttcgtaattg
    12721 gcgccgtcgg gcattttgat gattcggtct ttggccttca tggcggtgag ggcgctcatg
    12781 aagtttggat tgttggacgt gacattgaga atgccctgtt tgaaaccgaa aaagtagaat
    12841 tgttcggaag gtgatttgag ataaatataa agccggtcgt cgtcgttgga gggcattttg
    12901 aactcgatat agccggtgac gacacggttg atgctctcgc ccgcgatgga aacaagcccg
    12961 atgttttgct cggtgccgat gaacgactgg tactccacat cccacttgag tttgagacga
    13021 ctgaaaagga aggtgtaggg attgatgcgc ttgggtaggt ccaaatagcc ggaactgagg
    13081 ccgccgatgg cttcgcgggc gtctttatcg tcgggcagga gctgggtggc ggcgcggcgg
    13141 tagtagtcca gatcggtgag gtaaacgatg ggcgcggcat cgaaggtgga agaacggaaa
    13201 tcggtgacca tgactttggc caaggcgtcg gggagggtca tcgtgatgcc ggtcataagt
    13261 tcggcgcgca gtgggtccac ctgatttttg gtagtggcat ccggctcttc gtcgagcatg
    13321 acatcgccgg cgggcggggg tggaaacttg cctttagcaa agccggcagc gtccactttg
    13381 acgtacttga gggccgagcc gatgttgaag cgcccatcgg cctccacgct gccgtcggag
    13441 tttttgaacg tgaagaggtt gccgcgcacc tgactcgcaa caacgcgggt agaatcgccg
    13501 aaaatgaact ggtctttttc tttgatgtat ttgaaaaagc cggcagcggg gaagatgtcg
    13561 cgatccttgc ggaaggtgag cggctgcatg acggcggggt aaatattggc cgattctttg
    13621 ccgaggtaga gaccggtgta gagcggatcc ccattttcgt ttttaggtgt ggcgtagcga
    13681 atgatgaggt cgtttttgtc ggcctcgctg ctcaccgaga accattcggg gtagggcagc
    13741 ttgtcggcgt cgagcagggc gaagccttca aatttgagcg ctttggactc tgcattgagc
    13801 gaaatggtac ccttgaactt ggttttgtgg tctatataaa aatccttgtc ggcggccact
    13861 tcgcccgtag cgcgggtcac ggaggggcgc tcggagattt tgccccggcc cacgggctgt
    13921 cctacgatgt tttggaagtg aatttcctgg cctcgcgtgc cgatgttgta ctcgtaatag
    13981 ccctcggcct gataattgcg tttgccgcgt acgttgacgg tagcccggtt gatgacgtgg
    14041 ttacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnaggtcgt agtaagcagt ctcgccttca aagttgaggc
    14221 cgtcctgttc gggatgtacg gagcgaaact tacccttgat gcctttttcc gacttgaata
    14281 cgatggtttc ttcgtccata ttccaggtgt attcgttgag cgaagtctgg tactggttgt
    14341 aggggaagtc ggtccattcg ccgggtgtat tcgccttgaa cttgccgatt tttttatcaa
    14401 aatccacgtc gctgttcacg tttttggtgc gaatggccac gccctccacg ccgatggtgc
    14461 ggatgtcaat attggaggtg tcggccttga cggaattggc gccgaaggac agcagtttgg
    14521 agcgcatacc ggccttgtcc cactccaaca ggccattgcc cttgaggccg cccggcgtga
    14581 gaatgagggt gccggctacg gtgtgtttgc ccgactggaa aatggcaaag ggcgccgttt
    14641 cggaactgat gtacatgctg tcgcggtagg gccgccagag gatagacacc gaatcgccgt
    14701 gtacctgcgg ggtttgcacc gcccccgaac gcgactcttt catgtcgaag atgtcggctt
    14761 tggccagcat ttcggagggt ttaaacacga tgtctttggc gttcagttgt gcgccgaggt
    14821 agctgaggtt gcccttgccc agcagcccct gattgctcag ttctatggcg ccggaatagt
    14881 tgcctttgcc gcggtaggca ggcagtcctt tgtcgggggt gttgctgatg aagcccagcg
    14941 attggtcttt ttgcaaagaa atgctttcgg caaagggcgg gaagatgtcg gaagagagca
    15001 gtttgccttt aaaaacaagg tctttggcgg ccagcacgtc caaatgctgg aaactgaaag
    15061 gcgccagttc aaaataaaat gaatcgcgga catacacgcc ctgttgggtt tcgggtcggt
    15121 cataaaagac gaaagatttg gatttgctct ccaaagaagg gaagagcacg atgttgtcgc
    15181 ggcccgattt gttgccgggt gcgtcaatga gcagggcgcc ggtgaggtac tcgatgcgcg
    15241 actgtatgcc gaaggcgaca gggttgcggt ttttatcgaa ttcgccggta ggcaggaaga
    15301 tgtcgaaata gcgcacggag tctaaatgga cgaggaattt gttgtaatcg aagcggaatt
    15361 tcttgccttc cagcaccgta aatccggcga aaagccgccc gttgaagtcc atgttgcggt
    15421 cggcgcggag ggttatctcg ctgctgtcgg gtttgagggc cactttttgg cgggtgctga
    15481 act
//
LOCUS       DAORLF010000046        15478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2050_length_15478_cov_2.000324, whole genome shotgun sequence.
ACCESSION   DAORLF010000046 DAORLF010000000
VERSION     DAORLF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2050_length_15478_cov_2.000324"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1339
                     /locus_tag="PK198_06120"
     CDS             155..>1339
                     /locus_tag="PK198_06120"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF38341.1"
                     /translation="MPKQYLGYNDQDRRSDYAAFYHETLADLPAAVSAALSTGALPWG
                     ALPDVGDLPSLEKTGYAEVENGYAVNKDGSVAVAVRTEMPDVTPAMWDWWFGWHGSQD
                     LRYKLWHPGAHLSARWEDGKNDACYVGRNSIIREYIGKEPLDAVIQFKSPLDFGFSYE
                     AVNRKDQAVYICAKLGHPDLPVDYGYLVHQVRATANGAEMRSRFFIGGRYAGVRKRGK
                     LAAMVTDIVRRFKSLPENFGADLLRHCAEEMNHLAGFLPALYRQYHSDTQLAVNGEVY
                     HRAQEDFNKIILSNLYNKMDTGRIPDMLTEPRTVEDIIHIVRYAKKTGKRVTVLSGGR
                     SFSANSIRPDCIVISMKHFDQYKVNKEAMSAEAGPAVGGSVLLKALFKMGLFFPAGHC
                     TGV"
     assembly_gap    1340..1375
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1376..2312
                     /locus_tag="PK198_06125"
     CDS             <1376..2312
                     /locus_tag="PK198_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38342.1"
                     /translation="LQGGYGWNGRKTGIACQSILGMDIITADGELVHANETENADLFW
                     AARGSGPGFFGVVVKFYLKLYPLPPYRAIIAHDFSIQHLEDVYRWAYDVGPDIPKAVE
                     FQMLMSKNMMNVMGPGIEAFAPIFADTEDEFEAAKAFMHNSPIKKKAIVATPAFNPGI
                     DVFYRATMTHYPANYHYGVDNMWTHAPIDELLPFIKKIAETLPPPPSHFLWLNWYPDP
                     LSVDMAYSVEDNIYLALYSSWKNARDTEKYNFWAADMIREMQHLSSGIQLADEGLHKR
                     TDHFLKPEHLKRVQEIRAKRDPQGVFFEWHSKPGA"
     assembly_gap    2474..2505
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2514..>2741)
                     /locus_tag="PK198_06130"
     CDS             complement(2514..>2741)
                     /locus_tag="PK198_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38343.1"
                     /translation="MIFILMGGLFTPVDSMPEWAQWIARANPVAYFIEVVRLVVLKGS
                     SFYDIRGHFLVITGMALFFNVWAVWNYRKTS"
     assembly_gap    2742..2752
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2753..3640)
                     /locus_tag="PK198_06135"
     CDS             complement(<2753..3640)
                     /locus_tag="PK198_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38344.1"
                     /translation="MRTLRFLLQKEFLQIFRNKIILRIIVVLPVMQLLILPLAADYEI
                     KNIRISIVDHDRSAYSRQLINNIVASGYFSINDYSAAFEQSFEEFQKDHSDLILEIPA
                     RFEADIANQKGSEVLIALNAINGVKASVGSTYLSRIIGAFNADIRFRDLPQPRYPAVA
                     AIEVVPQFWYNPLMKYRVFMVPGILVMLVTAMASFMCALNIVKEKEGGTIEQINVTPI
                     RKYHFILAKLIPFWVIGMFVFTLGLLGVARLVYGIVPVGSLLTLYAFLAVYLVAMLGV
                     GLIISTYSATQQQSMSLAFF"
     gene            complement(3637..>4398)
                     /locus_tag="PK198_06140"
     CDS             complement(3637..>4398)
                     /locus_tag="PK198_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38345.1"
                     /translation="VQLIADASDPNTANTLVNYASAIIMDYQRERMQAAAIPVPVSTE
                     VRMIYNPELKSVTNFVPGVMALILLLICVLMTSVSIVKEKELGTMEVLLVSPFHPLLV
                     IISKAVPYFFLSLINLTVILILSVTLMGMPIAGSLWLLYGVSSIFIMAALSLGLFISN
                     VTHSQEAAMLISLLGMLIPTLLFTGFMFPIENMPLPLQVISNIVISKWYYIIVKSVMI
                     KGLGLASVWKETLILVFITVLLLTMSARTFKNRLA"
     assembly_gap    4399..4472
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4473..4740)
                     /locus_tag="PK198_06145"
     CDS             complement(<4473..4740)
                     /locus_tag="PK198_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38346.1"
                     /translation="MKQFLTFVRKEFYHVFRDRKTLLLLFGMPVMQIVLFGFALSNEI
                     KNARVAVYDLANDAASRRIIEKMEGGGKFEMERKAWNREGVEQAF"
     gene            complement(4744..5490)
                     /locus_tag="PK198_06150"
     CDS             complement(4744..5490)
                     /locus_tag="PK198_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF38347.1"
                     /translation="MNREIVIQTERLSKHFGDFVAVNEISFEVYAGEIFGFLGANGAG
                     KTTAMRMLCGLSIPTSGQARIAGYDVYKNTEDIKKNIGYMSQKFSLYEDLTLEENIRF
                     FGGIYGMSSRQIKAKSDALVEKLGMENETKKLVGALPLGWKQKIAFSSAIFHDPKIVF
                     LDEPTGGVDPITRRQFWDLIYEAADRGITVFVTTHYMDEAEYCNRLSMMVDGAIKALD
                     SPANLKEQYRAASMDEVFYEMARGAQRKAD"
     gene            complement(5487..>6056)
                     /locus_tag="PK198_06155"
     CDS             complement(5487..>6056)
                     /locus_tag="PK198_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF38348.1"
                     /translation="KDIYEHIEPFKKRRAGKLSGGMKQKLALCCALIHRPEVLFLDEP
                     TTGVDAVSRKEFWGMLRRLKEQGITILVSTPYMDEATLCERIALIQNGRIMSVETPDN
                     IIASYPHALYAIRSENMNALLHDLRRAGEVASSHSFGDHHHITFRAGGEEAVQQNLLQ
                     RLREAGHRELSLQPIRPTIEDCFIQQMPA"
     assembly_gap    6057..6158
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6159..6365)
                     /locus_tag="PK198_06160"
     CDS             complement(<6159..6365)
                     /locus_tag="PK198_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF38349.1"
                     /translation="MRAVEDVSLEVDKGELFGLIGPDGAGKTSIFRILTTLLLADSGS
                     AAVHGFDVVKDFRAIRSRVGYMPGR"
     assembly_gap    6374..6401
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6609..7610)
                     /locus_tag="PK198_06165"
     CDS             complement(6609..7610)
                     /locus_tag="PK198_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF38350.1"
                     /translation="MEKIRYFVWAAVAAISLAGCAGGEEKMDANGSFEAEERVISAEA
                     TGKILRLDIEEGQLLQAGAAVGQIDVDNLKLQAGQVSASMEALHKKTNDAGPQVAVLE
                     AQLAAHSRQMASLSKQLEVVDKEVARFKTLVASDAATQKQLDDLTGQRAVLEKQFSAA
                     ESQAEVIKAQIRAAKAGVQTQNTGILSEELPAQKRLEIIEKQIRDGAVLNQYAGTVLA
                     QYAREGEFTAIGKPLYKIADLSEVYLRAYITGDQLPQVKLGQEVKVLTDDGQGGYHET
                     SGTITWISDRAEFTPKTIQTKAERANMVYAIKVKVKNEDGRYKIGMYGGLKWGAQGG"
     gene            complement(7619..8899)
                     /locus_tag="PK198_06170"
     CDS             complement(7619..8899)
                     /locus_tag="PK198_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF38351.1"
                     /translation="MDIRILYARRVRRIFVLCLASLWALGLTGQSLSLDSCYAMARRN
                     YPLVQQFDLIEKSKALSLENAAKGYLPQLVNGQATYQSEVTRLPISFPGLNVPELSKD
                     QYRLFGEVNQPLTDLFFVKEQQDLIRANAALETQKTEGELHKLRERVYQLYFGVLYMD
                     ARLEQAQLLIKDLEAGLAKVQAAVAGGVALKSQADQLRAELLKARQQIAELKSGRRAY
                     AEALGLLTRQNMDEKTVLETPAAPLPATEIRRPELAMFGAQQQSLVQQSRLINNKTLP
                     RLSAFFQGGYGRPALNVLSNDFDPYFITGLRLNWNISSFYTSGNERQLLSTQQSAIAL
                     QRETFLLNTRLAVQQQQAEIAKWQELIVSDGEIILLRESVKNTAQQQLSNGTVTTTDY
                     LNYVNAEDLARQQLILHRMQLLLAQYNHLLTTGN"
     gene            complement(8951..>9391)
                     /locus_tag="PK198_06175"
     CDS             complement(8951..>9391)
                     /locus_tag="PK198_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF38352.1"
                     /translation="MLETMMGFMGAMMFVFNDEGSSFEEKVEKTVSSYIDLFIKEPQI
                     PLFIMNELQRNPEGLAGKLNIKSVLLNSVFARQYQEVMNRRGGEPLHIMQFVMSLMGL
                     LVFPFMGRPMLKIIGDLNDEKFVTMMKERKKLVPVWIEAILSAG"
     assembly_gap    9392..9570
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9792..10220)
                     /locus_tag="PK198_06180"
     CDS             complement(9792..10220)
                     /locus_tag="PK198_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38353.1"
                     /translation="MSEFKEVVAEGAREVGRKTLRITKKVLILLLLLGLVGAGGYYGW
                     GNMTYSSGTRSGQLMKLSRKGYVFKTHEGQLFLGVYTPSQPAMAPGNIWEFSVKNKQV
                     YEQIQKLEGKQVKLYYKEHFRTFPWQGDTKYFVYQAEVIK"
     gene            complement(10368..>10908)
                     /locus_tag="PK198_06185"
     CDS             complement(10368..>10908)
                     /locus_tag="PK198_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38354.1"
                     /translation="ASPLVKVTIVPRGIGTLGYAQYLPKEEYITRTEQILDRICMTFG
                     GRAAESIIFDKVSTGAQSDLDQVTRMAYSMVTVFGMNDKVGQVSFYSLGQDQFQRPYS
                     DTTASMIDEEVRRLLDVQYERAKELLREHRHHLELLARTLLDKEVLLKSDLERLIGPR
                     PYAEHPPLEHNSIPLAEEV"
     assembly_gap    10909..11089
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11090..12323)
                     /locus_tag="PK198_06190"
     CDS             complement(<11090..12323)
                     /locus_tag="PK198_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRF38355.1"
                     /translation="MEKNQNEDSKPGGTGGVFNSFWIYGIILAVLLVVHFATNGQVQK
                     VRLSTREFFDLVVKQEVARVQVQEDSKKAFVYIKEEYLSDAKHKKVAGNGSGEGRYHY
                     LVETGPIDQFQDELKKKQESAGIEVPLMADFVSPGIWPVIMTWLIPIGLILLVWLIIM
                     RRMAGGPGGPGSQIFNIGKSKATLFDQGTKVDVTFADVAGLNEAKEEVMEVVDFLKNP
                     KKYTSLGGKIPKGVLLVGAPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASR
                     VRDLFRQAREKAPCIVFIDEIDAIGRARGRSNIQGGNDERENTLNQLLVEMDGFPTDK
                     GVILMAATNRPDILDQALMRPGRFDRQIGIDLPDLQGRQAIFSVHLKTIKLADDVSPF
                     ALAEMTPGFAGADIANVCN"
     gene            complement(12418..12807)
                     /gene="rsfS"
                     /locus_tag="PK198_06195"
     CDS             complement(12418..12807)
                     /gene="rsfS"
                     /locus_tag="PK198_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRF38356.1"
                     /translation="MESQIQLLAQPPVGAEELNDLIVDSMQDIKAKRIVKLDLRHLKD
                     TPADYFIVCEGDSTTQVRAIAANVARRVKTEGHIRPAAKEGEQVARWICIDYYNTVVH
                     VFYRETRAFYELESLWSDAIFTEYETL"
     gene            12904..>13094
                     /locus_tag="PK198_06200"
     CDS             12904..>13094
                     /locus_tag="PK198_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRF38357.1"
                     /translation="MGKVLLDFPVLPSTNLFALDMLSEGRPDEGTVISARSQTEGRGQ
                     AGASWESEPGKNILLSVIF"
     assembly_gap    13095..13210
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13211..13634
                     /locus_tag="PK198_06205"
     CDS             <13211..13634
                     /locus_tag="PK198_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38358.1"
                     /translation="YIGDRKTAGILLQTSIKGFRFQYCVAGIGLNVNQTAFSEHLPNA
                     VSPAMLTGQDLDLHEVREDLLLCLEERYLQLRAGQTEAIREEYLRHLYRFDEPALFQR
                     KDGHIFEGRITGVTPQGLLQVRHGQGVSAFDVKEVAFL"
     gene            13688..13912
                     /locus_tag="PK198_06210"
     CDS             13688..13912
                     /locus_tag="PK198_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38359.1"
                     /translation="MEYIGLLFEILLTAIGVYVYLFSRGFVRSGNPETQEKAEAFRQS
                     NGWWLRIAGLALMAFMGSSMVLHIMELMRR"
     gene            14013..14267
                     /gene="rpsT"
                     /locus_tag="PK198_06215"
     CDS             14013..14267
                     /gene="rpsT"
                     /locus_tag="PK198_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRF38360.1"
                     /translation="MAHHKSSLKRIRQDEKRRDQNRFYKKTTRTAIKRLRGLEDATDA
                     QGFLPKVISMIDRLAKRGTWHKNKAANLKSGLMKRVAALG"
     gene            14620..>15478
                     /locus_tag="PK198_06220"
     CDS             14620..>15478
                     /locus_tag="PK198_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF38361.1"
                     /translation="MPDGSIAEWVAYNSVFRYIALSKRSKAFFEGKMGDDGSIRGQRT
                     FVDLETGNRKVGQKVYRYNIADGNWYEWEQDANEARKPMARLSGMESKVVVCNGKKFD
                     PASDLSGTWDGSNKALVYIFQDGDFLSIVHPQHFLDCKKTGRNSWKVHVYRTWGDCIT
                     EMSMNYECVTPDFINAVWVALDSRCDLHKGRTDRESYWRLKAMGTADSVFITEFRIFN
                     EFHPVDPKDYMAKELVIDHSQNYITFNFSSSYDPRTTTFYYQLEGFDKGWFIAFMQPS
                     ATYTNLDGGS"
BASE COUNT         3430 a         3914 c         3825 g         3550 t
ORIGIN      
        1 catagctcgt gccacgcccc ggcgtggtcg tagctttggg ggaccggctg tatagatacg
       61 aaaaaatata tccccacgca tttcttgaat taattcaaca tcgttgtctg tcccttgccg
      121 gcatttttgc atcaattttt aaaagcaaaa aaatatgccg aaacagtatc tgggttacaa
      181 cgatcaagac aggcgctccg attacgccgc attttaccat gaaacattgg ccgacctgcc
      241 ggcagccgtg agcgccgccc tcagcacagg cgcgctgccc tggggggcat tgcccgatgt
      301 cggcgatttg ccctcattgg aaaaaacggg ctacgctgag gtcgaaaatg gctatgccgt
      361 caacaaagac gggagcgtgg ccgtagcggt aagaactgaa atgcccgatg tgacacccgc
      421 tatgtgggac tggtggtttg gctggcacgg atcgcaagac ctccgctata aactgtggca
      481 tccgggcgca cacctcagcg cccgctggga agacggcaaa aatgacgcct gctatgtggg
      541 gcgcaattcc atcatcagag agtacatcgg caaagagccg ctggatgccg tcattcagtt
      601 caaatcgccc ctggatttcg gcttttctta cgaagctgtg aaccgaaagg accaggccgt
      661 ttatatctgt gccaaactgg ggcatcccga tttgccggtg gactatggct acctcgtgca
      721 tcaggtgcgg gctaccgcaa acggcgctga aatgcgctcc cgttttttca tcggcggccg
      781 gtacgcaggc gttcgcaaaa ggggcaaact ggccgcaatg gtcacggata ttgttcgaag
      841 attcaaaagc ctgcccgaaa acttcggcgc cgacctgctg cggcactgcg cggaagagat
      901 gaaccacctc gcagggtttt tgcccgcttt gtaccggcag tatcattccg acactcaatt
      961 ggcggtcaac ggagaggtgt atcatcgcgc ccaggaggat tttaataaaa tcattctcag
     1021 taatttatac aacaaaatgg acaccggccg catacccgac atgctgaccg aaccgcgcac
     1081 agtggaagac atcatccaca tcgtgcgata cgccaaaaaa acgggcaaac gggtgacggt
     1141 gctttccggc ggacgcagtt tcagcgccaa ttccattcgc cccgactgca tcgtcatttc
     1201 catgaaacat ttcgaccagt ataaggtaaa caaagaagcg atgagcgccg aggcagggcc
     1261 ggcggtcggc ggttcggtac tgctgaaagc gctgttcaaa atgggtttgt tttttccggc
     1321 agggcactgt accggcgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngttgc
     1381 aaggcggcta cggctggaat ggtcgcaaaa cgggcattgc ctgccaaagc attctcggta
     1441 tggacatcat taccgccgac ggcgaattag tacacgccaa cgaaaccgaa aatgccgatt
     1501 tgttttgggc ggcgcgaggc tccggtcctg gcttcttcgg ggtggtggtc aaattttatt
     1561 taaaactata cccactgccg ccgtaccggg ccatcattgc ccacgatttt tccattcagc
     1621 acctggagga cgtttaccgc tgggcgtacg acgtggggcc ggatattccc aaagccgttg
     1681 agtttcagat gttgatgagc aaaaatatga tgaacgtcat ggggccgggc atcgaggcat
     1741 ttgctcccat tttcgccgac accgaggacg agtttgaagc ggccaaagcc ttcatgcaca
     1801 acagtccgat caaaaagaaa gccatcgttg ccaccccggc cttcaatccc ggcattgatg
     1861 ttttttaccg ggccaccatg acccactacc ccgccaacta ccactatggc gtggacaata
     1921 tgtggacgca cgcccccatt gacgaactgc tgccttttat caaaaaaata gccgagaccc
     1981 tgccgccgcc gccatcgcat tttctctggc tcaactggta ccccgacccg ctgagcgtgg
     2041 acatggcgta cagcgtggaa gacaatattt atctcgcgct gtacagcagt tggaaaaatg
     2101 cccgcgatac cgaaaagtac aatttctggg cggccgatat gattcgggaa atgcagcacc
     2161 tttcatcggg catccaattg gccgacgagg gcctgcacaa gcgcaccgat cactttttga
     2221 aaccggaaca cctgaaacgg gtgcaggaaa tacgggcaaa acgcgatccg cagggcgtgt
     2281 tttttgagtg gcacagcaag ccgggagcct gagtgtgtat gtggccgaaa ccttgaaact
     2341 cagaaggatt tgaatgcgaa tggcgcggcg gttcccgcgt tgctcttggg ttttgcagtg
     2401 ttttgaggca cgggcgcgga gtagatatta ggcttagtag tggggcaaat tccgtagtgc
     2461 ggttttctac acannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcc gcatcaactc
     2521 gtcttccgat aattccacac cgcccacacg ttgaaaaaca gcgccatgcc ggtgatgacg
     2581 agaaaatgcc ctcggatatc gtaaaaactg ctccctttga gcaccaccaa gcgcaccacc
     2641 tcgatgaaat acgccaccgg attggcgcgg gctatccact gcgcccactc cggcatgctg
     2701 tccaccggcg taaacaaacc gcccatgagt ataaaaatca tnnnnnnnnn nngaaaaatg
     2761 ccagcgacat agactgctgc tgcgtagccg aataggtgga tataataagc cccacgccca
     2821 gcatggccac gagatatacc gccagaaatg cgtacagcgt gagcaatgag ccgaccggta
     2881 caatgccata taccaagcgg gccacgccca gcaatcccag cgtaaagaca aacatgccga
     2941 tgacccaaaa cgggatgagc ttggccaata tgaagtggta ctttcggatg ggcgtcacgt
     3001 tgatctgctc gatggtgccc ccttcttttt ctttcacaat gttgagcgcg cacatgaaag
     3061 aggccatggc ggtgaccagc atgaccaaaa tacccggcac catgaacacc cggtatttca
     3121 tgagcgggtt gtaccaaaac tgcgggacca cctctatggc ggccactgcc ggataacgcg
     3181 gctggggcag gtcgcggaag cgaatatcgg cattgaaggc gccgatgatg cggctcaggt
     3241 aggtactgcc caccgatgcc ttgacgccgt tgatggcatt gagcgcaatg aggacttccg
     3301 agcctttttg gttggcaata tccgcctcga aacgggccgg gatttccaaa atcagatcgg
     3361 aatggtcttt ttgaaattcc tcaaacgact gctcaaaagc ggcgctgtaa tcgttgatgc
     3421 tgaaatagcc cgaagccacg atgttgttga tgagttgccg cgaataggcc gaacggtcgt
     3481 ggtcaacgat ggaaatgcgg atgtttttga tttcgtaatc ggcggccaag ggcaaaatga
     3541 gcagttgcat gaccggcaat actacgatga tgcgcaggat gatcttgttg cggaatattt
     3601 gcaaaaactc cttttgtaat aaaaatctca aggttctcat gccaatcggt ttttaaaagt
     3661 gcgcgcgctc atggtcagca gcagcacggt aatgaaaacc agaatcaggg tttctttcca
     3721 caccgacgcc aagcctaaac ccttgatcat gacggacttg acaatgatgt agtaccactt
     3781 ggaaatcacg atgttcgata tcacctgcaa gggcagcggc atgttttcga tgggaaacat
     3841 gaagcctgta aaaagcaatg tgggaatgag catacccagc agtgaaatca gcatggccgc
     3901 ctcctgtgaa tgcgtcacat tggaaatgaa caggcccagc gacagcgccg ccatgatgaa
     3961 tatggaactg accccgtaca gcagccacaa actgccggca atgggcatcc ccatcagggt
     4021 gacgctgagg atgaggatca ccgtcaggtt gatgagcgag aggaaaaagt acggcacggc
     4081 tttggaaata atgaccaaca agggatgaaa cggcgacacc aacagtacct ccatcgtgcc
     4141 caattctttc tctttcacaa tggaaacgga ggtcatcagc acacagatca gcagcaaaat
     4201 gagcgccatc acgccaggca caaaattggt cacgctcttc agttcggggt tgtaaatcat
     4261 gcgcacctcg gtactcaccg gcacgggtat ggcggcggcc tgcatgcgct cgcgctgata
     4321 atccatgatg atggccgagg cgtagttcac cagcgtattg gccgtattgg ggtcggaggc
     4381 gtcggcgatg agttgcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggaaagcc tgctcgacgc cctcgcggtt
     4501 ccaggctttt cgctccattt caaattttcc gccgccctcc attttttcta tgatgcggcg
     4561 cgatgcggcg tcgttggcca gatcatacac ggccacgcgg gcatttttga tttcgttgct
     4621 gagggcaaag ccgaacagca caatctgcat caccggcata ccaaacaaca ggagcagcgt
     4681 tttccggtcg cgaaaaacgt ggtaaaactc cttccttaca aatgtcagaa actgtttcat
     4741 aactcaatcc gcttttcgtt gcgcgcccct ggccatttca taaaacacct cgtccataga
     4801 ggccgcgcgg tactgttctt tcaaattcgc cggcgaatcc aatgccttga tggcgccgtc
     4861 caccatcatc gagaggcggt tgcaatactc ggcctcgtcc atatagtgcg tggtgacaaa
     4921 cacggtgatg ccccggtcgg cagcctcgta gatcaaatcc cagaactgcc gccgcgtgat
     4981 ggggtccacg ccgccggtgg gttcgtcgag gaatacgatt ttgggatcgt gaaaaatggc
     5041 ggaggagaag gctattttct gtttccagcc caaaggcagg gcgccaacca attttttggt
     5101 ttcgttttcc atgccgagtt tttccactaa ggcatccgat ttggctttga tctgccggct
     5161 gctcatgccg taaatgccgc cgaaaaagcg aatgttttcc tccaatgtca ggtcttcgta
     5221 cagcgagaat ttctggctca tgtagccaat gttttttttg atgtcctcgg tgtttttgta
     5281 aacatcgtag cctgcaatgc gggcctggcc ggaagtggga atggaaaggc cgcagagcat
     5341 gcgcatggcg gtggttttgc ctgctccgtt ggcgcccaga aaaccaaata tctcgccggc
     5401 atacacctcg aaactgatct cgttgaccgc cacaaagtcg ccgaaatgct tgctcaggcg
     5461 ctctgtttgt atgactattt ctctgttcat gccggcattt gttgaatgaa acaatcttca
     5521 atcgtgggtc tgatgggttg caaactcaac tcccgatgcc ccgcctcccg caagcgctgc
     5581 aacaagtttt gctgcacagc ctcttccccg cctgcccgga aggtgatgtg gtggtgatct
     5641 ccgaaggaat gactggaggc tacctctccg gctcggcgca gatcgtgcag cagcgcgttc
     5701 atgttttcgg agcggatggc atacagcgca tgcgggtagg aggcaatgat gttgtcgggt
     5761 gtttctaccg acatgatgcg cccgttttga atcaaggcga tgcgctcgca cagggtggcc
     5821 tcgtccatgt acggcgttga gaccaaaatg gtgatgccct gctctttgag gcgccgcaac
     5881 atgccccaga actccttgcg ggaaacggca tctacgccgg tggtaggttc gtccaaaaac
     5941 agcacttcgg gccggtggat gagcgcgcaa cacagggcta atttttgctt catgccgccc
     6001 gacagcttgc cggcccgccg ctttttgaag ggttctatgt gttcgtagat gtctttnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ggccaggcat gtaccccacc
     6181 cggctccgga tggccctaaa atctttcacc acatcgaaac catgcacggc agccgagccg
     6241 gaatctgcca acagcagggt ggtcagtatg cggaaaatgc tggttttgcc tgcgccgtcc
     6301 ggcccgatga gaccgaacag ttcgccctta tccacctcca gcgacacgtc ttctacggcc
     6361 cgcacagccc cctnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttccagca ctacgtcttt
     6421 cataataaac ctgagttgtg aatggctata atttgcgcgc tcgcgcgagg ttctacctcg
     6481 cgtgtactat catgcgggtt ctacccgcgc tattgctgaa ccattacgtt tacgaatcct
     6541 tgaaggcttc gcaaacgctg ctcgccgctc gcgcgaggct ctacctcgtg cggactatcc
     6601 ggcgggttct acccgccttg cgctccccac ttcagcccgc cgtacatgcc tatcttatac
     6661 cttccgtcct catttttcac tttcaccttg atggcgtaca ccatattggc cctttccgct
     6721 ttggtctgta tcgttttggg tgtgaactcc gcccggtcgc tgatccaggt gatcgtgccg
     6781 ctcgtctcgt ggtagccgcc ctgcccgtca tcggtgagta cttttacctc ctggcccagt
     6841 ttcacttgag gcagttggtc gccggtgatg tacgcccgca gatagacttc cgaaaggtcg
     6901 gcgattttat acagcggttt tccaatggcc gtaaactccc cttcgcgggc atattgggcc
     6961 agcaccgtgc cggcatattg gttgagtact gcgccgtcgc ggatttgttt ttcaatgatt
     7021 tccagccttt tctgcgcggg cagttcttcg ctcaggatgc cggtattctg tgtttgcacc
     7081 cccgctttgg ccgcccgtat ctgcgccttg atgacctccg cctgactctc ggcggcgctg
     7141 aattgttttt ccagtacagc ccgctgcccc gtcagatcgt ccaactgctt ttgcgtggca
     7201 gcgtccgaag caaccaatgt tttgaaccgg gctacttctt tgtccaccac ttccaattgt
     7261 ttcgacaaag aagccatctg ccggctgtgc gcggccaact gagcctccaa cacggccacc
     7321 tgcggacccg cgtcattggt ttttttgtgc aaagcctcca tcgaagcgct cacctgcccg
     7381 gcctgcaatt tcaggttgtc cacatcaatt tgcccaacgg cggcgccggc ctgaagcaac
     7441 tgcccctctt caatgtccaa acgcaggatt ttgcccgtgg cttccgcgct gatgacgcgc
     7501 tcttcggcct caaaagagcc gttggcatcc attttttctt ctcctccggc gcatccggcc
     7561 agactgatgg cggccacagc ggcccaaacg aaatatctga tcttttccat gttggtactt
     7621 aatttccggt tgtcaaaagg tgattgtatt gtgccagcag cagttgcatc cggtgcagaa
     7681 tcagttgctg ccgcgccagg tcttccgcgt tcacatagtt caggtagtcg gtggtggtta
     7741 cggtgccgtt gctgagttgt tgttgggctg tattttttac gctttcgcgc aacaggatga
     7801 tctccccgtc ggaaacgatg agttcctgcc atttggctat ttccgcctgc tgttgttgca
     7861 cggccaagcg cgtattcagg aggaaggttt cgcgttgcag ggcgatggcc gattgctggg
     7921 tggagagcag ttggcgctcg ttgccggagg tgtaaaaact gctgatgttc cagttcagcc
     7981 tcagcccggt gatgaagtaa gggtcaaagt cgttgctcag cacgttgagt gccggccggc
     8041 cgtatccgcc ctgaaaaaag gcgctcaggc gaggcaaggt tttgttgttg atcaggcggc
     8101 tttgctgtac cagcgattgt tgttgcgcgc cgaacatggc caattccggt cgtctgattt
     8161 cggtggcggg aagcggtgcg gcgggcgttt ccaataccgt tttttcgtcc atgttttgtc
     8221 gggtaagcaa acccagcgct tccgcgtaag cccgtcgccc ggatttcaat tcggctattt
     8281 gctgccgggc tttgagcagt tcggcgcgca actgatcggc ctgacttttc agcgctaccc
     8341 cgcccgctac ggctgcctgc actttagcca aaccggcctc caagtctttg atgagcaatt
     8401 gggcctgctc caaacgggcg tccatgtaca atacgccgaa ataaagctga tacacccgct
     8461 cgcggagttt gtgcagttcc ccctctgttt tctgggtttc cagcgctgca ttggcccgaa
     8521 tcaggtcttg ttgttctttg acaaaaaaca aatcggtgag tggctgattg acctctccga
     8581 acagccgata ttggtctttg ctcagttcgg gtacgttcag ccccggaaac gaaatcggca
     8641 ggcgggtcac ttccgattgg taggtggcct gcccattgac caattgcggc aggtagcctt
     8701 tggcggcgtt ttccaacgac agcgctttcg acttctcaat cagatcaaac tgctgcacta
     8761 aggggtaatt gcgccgcgcc atagcgtagc aagagtccag actcaggctt tggcccgtca
     8821 accccagcgc ccacaaactt gcgaggcaca aaacgaaaat gcgccttacc ctccgggcgt
     8881 ataagattcg tatatccatc agattagata ttttgattta atcatttgtt taaaatagca
     8941 acaaaaaaaa tcatcccgca ctcaggatgg cctctatcca gacaggaacg agctttttgc
     9001 gttctttcat catcgttaca aatttctcat cgttcaggtc gccgatgatt ttaagcatgg
     9061 gccgccccat gaaaggaaat accaacagcc ccatcagact catcacaaac tgcatgatgt
     9121 gcagcggttc gccgccgcgc cggttcatta cctcctgata ttgccgggca aaaacggagt
     9181 tcaaaagaac ggatttgatg ttgagcttcc ccgccagacc ctccgggttg cgctgcaatt
     9241 cattcatgat aaaaagcggt atctgaggct ctttgataaa taaatcaatg tatgaagaaa
     9301 ccgttttttc taccttctct tcaaaactcg acccctcatc gttgaaaaca aacatcatgg
     9361 cgcccataaa gcccatcatc gtttccagca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttatacctc gcaccgtcag gtgcgaagcc
     9601 gcttttgtca taccgtgcgc tgctaatcaa caaacagcgc cgaaggctat gcccaaaacc
     9661 tggcggcgca aggtataatg atggcgcaaa ggtaagtact tttgctgttt aagtcaagtg
     9721 atttaatcaa atgattaaaa aatatttcaa aaaacgccct tccaaccgcg atgcgaccgg
     9781 aaaggcgccc cctacttaat tacttcagcc tgataaacaa agtatttcgt atcgccctgc
     9841 caggggaagg tacggaaatg ctccttgtag tacagtttta cctgcttgcc ttcgagcttt
     9901 tgaatttgct catatacctg tttattcttc accgaaaact cccagatatt gccgggcgcc
     9961 atagcgggtt ggctcggcgt ataaacaccc agaaaaagct gaccttcgtg cgttttgaaa
    10021 acgtacccct ttcgggagag tttcatcagt tggccggaac gagtaccgga actgtaggtc
    10081 atgttgcccc agccgtagta gccgcctgcg ccgacaaggc cgagcagcaa caacaggata
    10141 aggacttttt tggtaatacg aagcgttttg cgccctacct cgcgggcacc ttctgcaaca
    10201 acttctttaa attcgctcat ggcttttggt ttattttgaa taaaaacaaa atgtcccggc
    10261 tttccggttg taaggtttca aaaaaaaaga ggccggcaac tccctcgttc cggcccctca
    10321 atatagtgaa ttagtacgtc tttggagacg ctgctccgtt tatttcgtca cacttcctct
    10381 gccagaggaa tgctgttgtg ttctaatggc gggtgctcag cataaggacg cgggccgatg
    10441 agcctctcca aatccgattt gagcaacact tccttatcta acagggtgcg agccagcagt
    10501 tccaggtggt ggcgatgttc gcgcagcagt tctttggcgc gctcatattg tacgtccagc
    10561 aaacggcgca cttcttcgtc aatcatggaa gcagtggtat cggaatacgg gcgctggaac
    10621 tggtcttgcc ccaggctgta aaaagacacc tgccccactt tgtcgttcat accaaaaacc
    10681 gtgaccatgc tgtacgccat gcgcgttacc tgatcgaggt cgctttgagc gccggtggac
    10741 accttgtcga agatgataga ctcggcagca cggcctccga aagtcataca aatcctgtcg
    10801 agaatttgct ccgttcgggt gatgtactct tccttgggca aatactgcgc atatcccaga
    10861 gtgccgatac cgcgtggcac gatggtcact ttgaccaaag gcgaggcgnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc attgcacaca
    11101 ttggcgatgt cggcgccggc aaaaccgggc gtcatttcgg ccaatgcaaa aggagagacg
    11161 tcatcggcta atttgatggt cttgagatgc accgaaaaaa tggcttggcg gccttgcaga
    11221 tcgggcaggt caatgccaat ctggcggtcg aagcggccgg ggcgcatgag ggcctgatcg
    11281 agaatatcgg ggcggttggt ggcggccatc aggatgacgc ctttgtcggt aggaaagccg
    11341 tccatttcta ccaacaactg gttgagcgtg ttctcgcgct cgtcgtttcc gccctgaata
    11401 ttggagcggc cgcgggcgcg accaatggcg tctatttcat ctataaagac gatgcaaggc
    11461 gctttttcgc gggcctgccg gaagaggtcg cgcacgcggg aagcgcctac gccgacaaac
    11521 atttccacaa aatcggagcc ggaaatagag aaaaacggca cccctgcctc gccggctacg
    11581 gccttggcca gcaaggtttt gcccgttcct ggagcgccta ccagcagcac gcctttgggg
    11641 attttaccgc ccagcgaggt atatttttta ggatttttga gaaaatccac gacttccatc
    11701 acttcttctt tggcttcgtt gaggccggcc acgtcggcaa aagtgacgtc caccttggtg
    11761 ccttgatcaa agagcgtggc tttcgatttc ccgatgttga aaatctgaga acccgggccg
    11821 cccggaccgc cggccatgcg ccgcatgatg atgagccaaa ccagcaatat caggccgatg
    11881 gggatgagcc aggtcataat gacgggccaa atacccggag aaacaaagtc ggccatgagc
    11941 ggaacctcaa taccagcgct ctcctgcttt tttttcagtt catcctgaaa ctggtctatg
    12001 gggccggttt ctaccaaata atgatagcgg ccttcgccac tcccgttccc tgctaccttt
    12061 ttgtgcttcg catcagacaa atactcctct ttgatataaa caaaggcttt tttgctgtct
    12121 tcctgtactt gaacacgtgc aacctcctgt tttacaacca agtcgaaaaa ttcccttgtg
    12181 ctcaggcgca ctttttgtac ctgcccgttg gtggcaaaat gcaccaccaa aagcactgcc
    12241 agaatgattc cgtatatcca aaaggaattg aagacaccgc cggtacctcc gggtttgcta
    12301 tcttcgtttt gatttttttc catgccgtgt tttactttaa tgcaaggtct cgcacctgat
    12361 aattaaggga taaacgcgca tcgaggcgat tcgttgacgt gctgtccttt tataatgtta
    12421 caaagtttcg tactccgtga aaatcgcatc gctccacaac gactctaatt cgtaaaaggc
    12481 gcgggtttcg cgataaaaca catgcactac cgtattataa tagtctatgc aaatccagcg
    12541 ggctacctgc tcgccttctt tggccgcagg gcggatatgc ccttctgtct ttacccttct
    12601 tgccacgttg gcggcaatgg cgcgcacctg tgtggtagaa tcgccttcgc aaacaatgaa
    12661 ataatcggca ggtgtatctt tcaaatggcg caggtctaat ttgacaatgc gtttagcctt
    12721 aatgtcttgc atgctgtcaa cgatcaggtc gttgagttcc tcggccccca caggaggttg
    12781 ggcgagtaat tgaatctgtg actccaagtt gtgattttat tatttttgcg gttgaataca
    12841 atggtttaaa agtgtacaaa ttacaaactc tttttccaat tgcgcaatag gaacactctt
    12901 tttgtgggaa aagttttgct tgattttccc gtccttccct ccaccaattt gttcgcattg
    12961 gacatgcttt cggaggggcg gcccgacgaa ggtacggtga tttctgcccg cagccaaacc
    13021 gagggcagag ggcaggccgg cgcctcatgg gaaagcgaac cgggcaaaaa catactgctg
    13081 agtgtgattt tctannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn atatataggc gaccgaaaaa cggcgggtat tttgctccag acctccatca
    13261 aaggcttccg gtttcaatat tgcgtggcgg gcatcggcct caatgtaaat caaacggcct
    13321 tctccgagca cttgcctaac gccgtctcac cggccatgct gaccgggcag gatttagacc
    13381 tgcacgaagt ccgggaagac ttgttgctct gcctcgaaga gcgctacctt caactgcgcg
    13441 ccggccaaac ggaggccatc cgcgaggaat acctccggca tctctaccgc tttgacgagc
    13501 cggctctttt tcagcgcaaa gacggccata tcttcgaggg gcgcatcacg ggcgtaacgc
    13561 cgcaggggct tttgcaggtg cggcacgggc agggcgtctc cgcttttgat gtgaaggaag
    13621 tggcgttttt gtgaatggtt tatacctatc ttgcatccgt ttccgtcaac gtttaaattc
    13681 tgccatcatg gaatacatcg gtttactttt tgaaatcctg ctcactgcca ttggcgtgta
    13741 tgtgtatctt ttttccagag gctttgtgcg ctccggcaac ccggaaacgc aggaaaaagc
    13801 ggaggcattc cggcagtcca acggctggtg gctgcgcatt gccggcctgg ccctaatggc
    13861 ttttatgggg tcttctatgg tgctgcatat catggaattg atgcggcgtt gacggcatgt
    13921 caagatattt ttcaaaaaag atttgacttc ttcaaaagaa aagacctaca tttgcgctcc
    13981 gttttaaaaa gcaaaaaaga aacatacgga ttatggcaca tcataaatcg tcattgaagc
    14041 gcattcgtca ggatgagaag cgccgcgatc aaaaccgttt ttacaaaaag accacccgca
    14101 cggccatcaa gcgcctgcgc ggtctggaag atgcaacaga tgctcagggt tttttgccca
    14161 aagtgatcag catgattgat cgcttggcca agcgcggcac ttggcacaaa aacaaggcgg
    14221 ccaacctgaa aagcggtctg atgaagcgcg ttgctgctct gggatagtcc ctgtttaaaa
    14281 agatttaaga tacttcaacg agtagtcctg taacagcagt tgcaggacta tttgtgtttt
    14341 ggggcaaggg ccgatgaatt tcgctggagg aaagcccggc ccggcggcat agggtggcgc
    14401 aacatgcggg taggcgccgg gggctggggg ctgagttttt gatgaaaaaa atgggcaaag
    14461 cggtacattt gtctcactta aaattctgat aaagtgaggt gtatattgct catatcaatt
    14521 agcctgttgt tcgtaaacaa ggcactgagt cgggtcaact cgtcggtgtc ccctaccaag
    14581 ttaaaaacat caagttcaaa tattataggc acttggagaa tgccggacgg cagcattgcg
    14641 gagtgggtag cctacaattc ggttttcagg tatatcgcac tgtcaaaacg ttcaaaggca
    14701 ttttttgaag gaaaaatggg agacgatggc agtatcaggg ggcagcgtac ctttgttgac
    14761 cttgaaacag gaaatcgaaa ggtcggacag aaggtttatc gctacaatat tgcggatggc
    14821 aattggtacg aatgggagca ggacgccaac gaagctcgta aaccaatggc ccggctgagt
    14881 ggcatggaaa gcaaagtcgt ggtttgtaat ggcaaaaaat tcgacccggc aagcgacctg
    14941 tcaggaacat gggatggaag caataaagca ttggtttata ttttccagga tggggatttc
    15001 ctctccatcg tacatccaca acatttcttg gactgtaaaa aaacagggcg taactcatgg
    15061 aaagtccatg tttatcgaac atggggcgat tgtattacgg aaatgtccat gaattacgaa
    15121 tgtgtaacac cggatttcat caacgcagta tgggtagcat tggatagtcg ttgcgacctg
    15181 cacaaagggc gaactgacag agagtcttac tggaggttaa aagcaatggg aactgctgac
    15241 tccgttttca ttaccgaatt tcggatattc aatgaatttc atcccgttga ccccaaagat
    15301 tatatggcca aagaattggt gattgaccat agccagaact acatcacttt caatttctcc
    15361 agttcctacg accccaggac gacaactttt tactaccaac tcgagggctt cgacaagggc
    15421 tggttcatcg catttatgca gccctcggcc acctacacca acctcgacgg cggcagct
//
LOCUS       DAORLF010000047        15279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2083_length_15279_cov_2.085654, whole genome shotgun sequence.
ACCESSION   DAORLF010000047 DAORLF010000000
VERSION     DAORLF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_15279_cov_2.085654"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PK198_06225"
     CDS             <1..1014
                     /locus_tag="PK198_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38362.1"
                     /translation="EHGEARLFVLLDERNEPAGRVAAFIDHARNKNLPYPVGGIGFFE
                     CVDNAAFAKALLSKAEGWLLSRGAAAVDGPVNFGERDKYWGLLDKGFDPPLFQENYQP
                     SYYKAFFTDNGYIPFEQILTLKGNTADIPVERMKMVADRIRRTNNVHLEKYHPSQLDR
                     YAVDFCEVYNASFRHFQHFKPIVPEQMKRVMKQAQPILDPNLLCIAYFEGKPAGFCAF
                     FPDINPLLRPVKGKLSGLNVLKFLWAKRNTREFQAKGIGFGIHPDYKTKGIFAFITEF
                     MATPQNMAIYKDMFLTTVRAHNSEAVSIYLKLAVKIDRVHIAYRKALKDDVVVEPHEF
                     MEV"
     assembly_gap    1191..1221
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1222..1800)
                     /locus_tag="PK198_06230"
     CDS             complement(<1222..1800)
                     /locus_tag="PK198_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38363.1"
                     /translation="MSYNLGQIFHLFHNGLGNNFNARDLWGVGNNVFVLTNDQLWRRD
                     GAQWSPASAGLFDGTGNIPLIYDINGDSANALIVGKLQNSSQPLLFLSHDGGVSWGDN
                     LAEGLPDMSYEFRALLHSEAIYVIGHPEGVLRWSIWKRSGAVATTEPAEVAALHLFPN
                     PAATSVWLRCDQIAAVGGRIVIHDFTGRAVWSR"
     assembly_gap    1864..1880
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1881..3236)
                     /locus_tag="PK198_06235"
     CDS             complement(<1881..3236)
                     /locus_tag="PK198_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38364.1"
                     /translation="MKMPIITAFAVVLLCLFAPMPAFSQWTEIPIPFGPPMAGRMTTY
                     QGKIFTTVYNYDRVKCFRTDDYKRWSLAADFPGSHNFGKAGLEVDGNKLYLISSNATQ
                     LSADAWSSDDGGQSWQELNLPNSAPLFMIPRNDVLLCASVYNVQRSTDGGAHWSGALW
                     LNDRIRDMKLLQDQTVLLITDRYIYRSTDHGLTWDYSPAPYNVANGIIPIVTIFPTEH
                     GVFLKVQIGSQVSLYRSMDNGANWSSASFPAFSGSPTVNDLRSIGGSLWGVFSNRIAY
                     SEDDGDTWEWVVTPHSTDQICNLGDTIFAGGHLGFYKSYDRGHTWLTGNAGWADAGAT
                     FNAYLWEEKLYASGNKIFLSTNEGLFSNSNDGDEWLENINGRMSYLYQQGDTLVFLGK
                     GSLRSFDGGDSWAYSPASTVSPWLPTDFYDYTRINNRIFTNGTIHYSDDLGVTWHRIT
                     AL"
     gene            complement(3396..>3594)
                     /locus_tag="PK198_06240"
     CDS             complement(3396..>3594)
                     /locus_tag="PK198_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38365.1"
                     /translation="GIVIASPPAWYLMRRWLQDFAHRIEMEWWIFVASGALSVVVATL
                     VVAMTAGRAALADPVRSLKSE"
     assembly_gap    3595..3628
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3629..>5817)
                     /locus_tag="PK198_06245"
     CDS             complement(<3629..>5817)
                     /locus_tag="PK198_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38366.1"
                     /translation="AWRNLLRHRSFTFINLFGLSIGMAAALLILKYVAYERSYDKIAP
                     GTDLIWRAFNETLSEGRVATQDCNTHSALGPALKTDMPEVADFFRLFNSNQHETVFFQ
                     NNEPVRLPHVWMADPGFLRMFPQDFVAGNANSCLSQPGQILLSRSAAARLFPEGQAMG
                     QTLRIPRGQLRGEYVVSAVVNDPPQNTHLKFSALVSYATRYAGGHEDNWTGYWDYNYF
                     QLKPGADPEKVRRQLNVYSQQHLSDAGIRLNMQAFEDIHLQSNMTYEIEPNGSARTVG
                     FLLAAALFVLAIACINYVNMMTARSLERSKEVGLRKVVGAGRGRLFSQFLVEGLLLNA
                     GAMLTALGIFQMALPAFGQLMGRPLADARLDMTFSGAVAGIFMVGVLASCAYPAWTLT
                     RFSPLSVMSGAIPLVSADSKGSWLRKSLVVIQFGFTVGLIFSVMVVSRQLNYLHRHDK
                     GLSLDQILAVRNSAYDWREDSLNRRGMAVFKNEIRQISGIRQVAASSITPGLGINNIS
                     GSSGGMVPAHRPDDVRPGTTYFVNAEPDFYATYGIRFLAGAPYRAPDVRSGHNHVIIN
                     ESALRMLGMENPEEAVGQEIAYAGSTDGYRMKIEGVVADFHIETLKEPTRPTVYFCLP
                     EVAEGYVSIKVETADVQTALAAMEQVWASVFPDSPFEYWFLNEQFARQYQAETQLSRV
                     FSGFAMLAVIIACLGLYGMASYLTARRTKEIGVRKVLGASVSGIVALL"
     assembly_gap    5818..5840
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5942..6970)
                     /locus_tag="PK198_06250"
     CDS             complement(5942..6970)
                     /locus_tag="PK198_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF38367.1"
                     /translation="MKYTPLPLLFMLFLATAEAQPAPGGGPEWDSIFNYAQGHSVLRD
                     SVNWAALRPTLDSVCASEGLIPATKRLLHELRDFHGKIWVDNVPHQGAVKPWTPTSMQ
                     LDTSLLHKLFRNELPVFAQRLPGRIGYIRVPGITFGERDSINARIIRQAMEGVQKGVK
                     LRGWIVDLRLNGGGTMFPMLAGLGALLGDSTEIGSFIHPDAGYKEAWTLRQGEVFVGE
                     YQVTDYGLWQKMGIPRSLSSLPVAVMISAATASSGEVTALAFRNRPRTRFFGEDTAGY
                     TTAVSWQPISDQVVLQLTESYYADRLENVFPGTPVLPDERCDGGENFYQITKDQMIQR
                     ALEWLKKQ"
     gene            complement(7130..>8064)
                     /locus_tag="PK198_06255"
     CDS             complement(7130..>8064)
                     /locus_tag="PK198_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF38368.1"
                     /translation="RVALGDALLSMSYSSNTHHYTDEAGNKKETNIYRKNADEELMDL
                     YKLPLLAGRKLQASDGPEHYVINEAAVKAFGFESPQAAVGSFIQENNGPDEPPRQKQI
                     VGVTADFHSMGFTEAIRPTAMMRDLESVGELNVRLASSNPSDWQIVLKKMETEWKRIY
                     ADEPFAARFYDETLADIYEQDLSLAKFIQLATGIAIFISFLGLFGLATFMAWKRTKEI
                     GIRKVLGASAVSVVGMLSREFVLLVLAGFVLSTPVAYFLLQKWLQFYAYRIELSWWIF
                     ALAGAAALVGATLVVALQTVRAALANPVESLKNE"
     assembly_gap    8065..8075
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8076..9584)
                     /locus_tag="PK198_06260"
     CDS             complement(<8076..9584)
                     /locus_tag="PK198_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38369.1"
                     /translation="MTSNQLKSTFRILWRGRLFTLLHIAGLAIGLSAAWLIWQYADFE
                     YSHNRLIPNAKNIFRVVSEFNDSDNINSHNAGCPQPLWRAAEEVAGIEKSVPIQSHFA
                     LLALPEGAKRGFKEVREMAKTTPEYFELVPVKWLAGSPATALSQPDEVVLARSRAEKY
                     FPRHSPEEILGKTIRYESFNDTLTVTVAGVIEDLDFPTSFIEKELLTVTEPDEERWRG
                     TNSNDQLWVILNPDADREAITTAINQVSQTKGGEALKQWGMSRTHQLQALADVHFADR
                     YGSHIRVANAKIIKILAAVAIFLLLLAVINYVNLSTAQIPARAREIGIRKPLGGSRRS
                     IIGSFLLETLLVCLLATALAGGLTRFAFDAFSEYLPEDVLRYADWRKTGAFLAVLVGA
                     VTLFSGLYPGWIASRFQAAALLRGQFSGQEQGSRKRGGNLRRGLIVFQFFVAQAFIIG
                     ALVVGQQLHFMRNSDLGFDRQAVLTIEQPMSAWRNPDLKNKFPVLADAFKKMP"
     gene            complement(9677..12052)
                     /locus_tag="PK198_06265"
     CDS             complement(9677..12052)
                     /locus_tag="PK198_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38370.1"
                     /translation="MFQYNLKVALRTLWRNKGFSLLNIAGLGIGLTVALLIGLWIANE
                     LSFDQFHEKGERLYQVKMSDYVNGELETWSSVPLAMAEELRKSFPEVRYVAEADWGWQ
                     HGLKYEDKLFFKSGFQVDDDFLKMFSFPLVAGSVETALDAPNSIVLTKEMAESLFGHK
                     NWSDMLGEVVRVDNYYDQKVTGVLENVPCNSSLSFNYLLPFSLYEDMDWVKDSRDDWG
                     NNSFQMFMELQPGVDGTAFAAKVKDIIRRNNKESKADVTLHALADWRLRSEFKNGRVA
                     GGYIEYVRLFGIIGMFVLLIACVNFMNLSTARSERRSKEVGVRKTLGSARGQLIGQFL
                     GEALLMAGIAMILSLLLLELVLPGFNLLTKKELSTPYGSPVFWALTLGFTTLAGLLAG
                     SYPAVFLSSFQPIKVLKGTFSGLGKAALLPRKVLVVSQFSVSIALIVSTLVVSQQIRH
                     AKNRPLGYDPDRVVMVVMSGDLLDNYLPLKNEMLASGLVTGVTKAGSPVTNIYSNNKG
                     VEWSGKDPSDEIIFATIPTAEDYFTTLGMTLKEGRFFSSDFKSDSTAIIFNESAIQRM
                     GLENPLEETVQWQGMTFRIVGVVNDVVMTNPYEKVSPAMFLSNPLWANDMMFRLNPEM
                     DTRQALAQLEPIFKKHNPAYPFDYTFADEFHSRKFATEELIGKLAGIFAGLAIFISCL
                     GLFGLSAYMAERRTKEIGIRKVLGASVSNLWALLSKEFVGLVLVSALVAAPTAAWFLK
                     DWLEKFEYRIGLPWWVFVAAGVMAVVGATLVVALQTVRAALANPVKSLRSE"
     gene            complement(12160..>12571)
                     /locus_tag="PK198_06270"
     CDS             complement(12160..>12571)
                     /locus_tag="PK198_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF38371.1"
                     /translation="EQMYQTEMQTTRIIGVFSWLAVALACLGLFALASYHIAQRTKEI
                     GIRKVLGASAAGITALVSKDFLVLVLIALFIASPFAWYLMSKWMLNFAYRIDIQWWVF
                     VVAGALSVVAATLVVALQAVKAALANPVKSLRSE"
     assembly_gap    12572..12581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12582..14566)
                     /locus_tag="PK198_06275"
     CDS             complement(<12582..14566)
                     /locus_tag="PK198_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38372.1"
                     /translation="MFQNHLKLAWRRLLNNRLFSAVQLVGLTAGISACLLIGLFIHHE
                     WTYDAMHAHADRIVKVNMEYSFGGEVVHSGSTGNKVAPTLAREFPEVEAAVRVMQYTE
                     VVKSGAMLTEEAQFFYADSTFFQIFSFPLLKGDASTALHAPWQVVLSESTARRYFGEA
                     DPIGKTLNIGKREAKVTGVMADAPANTHIKPDFVCSFVGLSAARPENETWWNANYATY
                     LLLKNPEGQALMQARLPDYMASKAHETGADDGSSYLKLHLLPLRDLHLRSTVPGDFEP
                     NGDVRYIYILGVAALLILLIGCTTYINLATATGMERAKVTGVHKVLGAGSRQLAAQHL
                     GEAAIVTFLALFIAFLTTAPLLPVFNRIFNRALTAEPLFQPAAWVALAVLGLAVSFGA
                     GFYPAVVAARFSPAAVLKGWAGNAKSGAWLRKTLVIAQFSISILLIICTLLLREQMQF
                     IQNKKLGFDQEQMLVLPADGEVVGQYGALKSQLLQLSEVQSVTMSYGTPSNIKGGYDI
                     SPTLLQQDARPVTALPAGLDFLKTMSIELVAGSDFNEADLATASRLRNDSTAVLPILL
                     NEAQVAAFGWSLEETVSKRLYFNGAPAMVKGVVRDFHFRSLHEAVAPLVIFPDGWGQY
                     IFVKLKGGDVAQSIERMGALWAGVAPHRPYSFHFLDE"
     gene            complement(14655..15020)
                     /locus_tag="PK198_06280"
     CDS             complement(14655..15020)
                     /locus_tag="PK198_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38373.1"
                     /translation="MKRMILPALFLLMVAGASAQTELPFWLTDRKWSLKEDVMSGMGT
                     HQSLPKHTQLEFFTNGQWAADEPIAGIREGIWKQGKNGKIMMRFSGKKEAVVQPVKDN
                     EIEIILKVKMKKLIWTWAG"
BASE COUNT         3682 a         4116 c         3939 g         3416 t
ORIGIN      
        1 gaacacggag aagccaggct cttcgtcctg ctcgacgagc gcaacgaacc cgccggtcgc
       61 gtggcggctt ttattgacca tgcccgcaac aaaaaccttc cctatcctgt gggcggcatt
      121 ggtttttttg aatgtgtgga caatgccgca tttgccaaag ccttgctgtc gaaagcggag
      181 ggctggctgc tgtcgcgcgg ggcggcggcg gtggacgggc cggtcaattt cggcgaacgc
      241 gacaagtact ggggcttgtt ggacaagggt ttcgatccgc cgctgtttca ggaaaattat
      301 cagccatctt actacaaggc attttttacc gataacggct acatcccttt tgagcaaatt
      361 ctgaccctca agggcaatac tgccgacatt ccggtagagc gcatgaaaat ggtggctgac
      421 cgcatccgcc gcaccaacaa cgtgcatttg gaaaaatacc acccctcgca gttagaccgc
      481 tatgctgttg atttttgcga ggtgtacaac gccagcttcc ggcattttca gcacttcaaa
      541 cccatcgtgc ccgaacagat gaaacgggtg atgaaacagg cgcaacccat tttggacccc
      601 aacctgcttt gcatcgctta ttttgaaggc aagcccgccg gtttttgcgc tttcttcccc
      661 gacatcaacc cgctgctgcg cccggtgaag ggcaaacttt caggactcaa cgtcctcaaa
      721 ttcctctggg ccaaacgcaa tacccgcgag ttccaggcca agggcatcgg tttcggcatc
      781 catcccgatt acaaaacgaa aggcattttt gcgttcatca ccgaatttat ggccacgccg
      841 caaaacatgg ccatttacaa agacatgttc ctcaccaccg tgcgggcgca taacagcgaa
      901 gcggtgagca tttacctcaa attggctgta aaaatagacc gcgtacacat cgcctaccgc
      961 aaagccctga aggacgatgt ggtggtggag ccgcatgagt ttatggaggt gtaggtttgg
     1021 ttctcctcac tcacttttca aacttctcac cggatcggcc ggccaatatt ggaaaacgag
     1081 aagtgaaaaa atgtcagcgt ataatcagtt tgcccacagc atacttgcca ctgggcgcca
     1141 tatatgatac agaataaacc cctggcaacc atccgttcac aggaatttcc nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn ngcgactcca aacggcgcgt ccagtaaaat cgtggattac
     1261 tattctcccc cctacagcgg ctatctgatc gcatctcaac caaaccgaag tagcagcggg
     1321 attggggaac aagtgaagtg ctgcaacctc agccggctct gtcgtagcta cagctcccga
     1381 acgcttccaa atggaccaac gcaatactcc ttctggatgt ccgatcacat aaatggcctc
     1441 actatggagc agggcacgaa attcgtagct catatcgggt agcccctccg ccagattgtc
     1501 gccccaggaa acgccgccgt cgtggctcaa gaataacaaa ggttgtgaag aattttgtag
     1561 tttaccaacg attaaagcat tagcactatc cccgttgatg tcataaatca aaggaatatt
     1621 gccggtgcca tcaaaaagac ctgcactggc ggggctccac tgggcgccgt ctctgcgcca
     1681 gagttgatcg ttggtcaaaa caaaaacgtt atttcctaca ccccataaat ccctggcatt
     1741 aaaattatta cctaaaccgt tatgaaacag atgaaagatt tgccccagat tataagacac
     1801 gaatatatca ccggacctga cagcgtatag tgcattccca acacctgcaa ccggctctgt
     1861 cggnnnnnnn nnnnnnnnnn caaggcggta atccgatgcc aggttactcc cagatcatca
     1921 gagtaatgaa tcgtgccgtt ggtaaagatg cgattattaa tccgcgtgta atcataaaaa
     1981 tcagtaggaa gccatggaga aacggtggaa gcaggactgt acgcccatga atctcctcca
     2041 tcgaaagagc gcagcgaacc tttacccaaa aaaacaagcg tatcgccttg ttgataaaga
     2101 taagacattc taccattgat gttctccagc cattcatcac catcattgga gtttgaaaat
     2161 agcccctcat tggtagaaag aaaaattttg ttgccgcttg catatagctt ttcttcccat
     2221 aaataggcgt tgaaggttgc gccggcatcg gcccaccctg cattacccgt caaccatgtg
     2281 tgcccccggt cgtaagattt ataaaacccc agatggccac ctgcaaaaat agtatctccc
     2341 agattgcata tctggtcggt tgaatggggt gtcactaccc attcccaggt atctccatcg
     2401 tcttccgaat aagcaattcg attactgaac accccccaaa gtgagccgcc aatgctgcgc
     2461 aagtcgttca cagtcggact gcctgaaaaa gcaggaaatg atgccgacga ccagttagcc
     2521 ccgttgtcca tagagcgata taaagaaacc tgagaaccga tctgcacctt gaggaatacc
     2581 ccatgctccg ttgggaaaat ggtaacaatg ggaataatgc cattagctac attgtaaggg
     2641 gccggcgaat agtcccaagt aaggccatga tctgttgatc tgtaaatata tctgtctgta
     2701 atcaacaaca ccgtttggtc ttgcaggagt ttcatatccc gaattctatc gttgagccac
     2761 agcgccccgc tccagtgagc gcctccgtct gtacttcgct gaacattata aacagaagca
     2821 cagagtaaga catcattacg gggaatcata aacagtggcg cagaattagg caagtttagt
     2881 tcctgccagc tttgccctcc atcatcgctg ctccacgcat cggctgatag ttgggttgca
     2941 ttagagctga tcagatataa tttattacca tctacctcaa gccccgcttt gccaaaatta
     3001 tggctgcccg gaaagtctgc tgccagcgac caacgtttgt agtcgtcagt ccgaaagcat
     3061 ttcactctgt cgtagttata gaccgtagta aaaatctttc cctgatatgt tgtcatacgg
     3121 cctgccatgg gaggaccgaa aggtatgggg atttccgtcc attgagaaaa ggccggcatt
     3181 ggggcaaaca gacataaaag caccacagca aaggcagtaa tgataggcat tttcataaaa
     3241 attgatttga gggcttaaaa ataacattac cagccatttt atgaaaataa tatttcaaaa
     3301 ccataccaaa ataaaacaaa gagaattctt tgggcagcct atcgcagaac ggtaaaagaa
     3361 gcatttcggc ttttcaaaaa cgggcaatta taacctcact cactcttcaa actcctcacc
     3421 ggatcggcca gcgcggcgcg gcctgccgtc atggccacca caagggttgc tacaacgacg
     3481 gacaaggcgc ccgacgctac aaaaatccac cactccatct cgatgcggtg ggcgaagtct
     3541 tgcagccagc ggcgcatcag ataccaagcc ggcggcgagg caatgacgat accgnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag caatgccacg atgcccgaaa ccgatgcgcc
     3661 cagcactttg cgcacgccga tctctttggt gcgccgcgcc gtcaggtagg acgccatacc
     3721 gtacagcccc aaacacgcta tgatgacggc cagcatggca aagcccgaaa acacgcggct
     3781 cagttgcgtt tcggcctgat actgccgggc aaattgctcg ttcaaaaacc agtattcaaa
     3841 agggctgtcg ggaaatacgg aggcccatac ctgctccata gccgcgagtg cagtctgcac
     3901 atcggcggtc tctactttga tggaaacata gccttcggcc acctccggca ggcagaaata
     3961 caccgtaggc cgggtgggct ctttgagtgt ttcgatgtga aaatcggcta ccacgccttc
     4021 tattttcatt cggtatccat cagtgctgcc cgcgtacgca atttcctgac ccacagcctc
     4081 ttccgggttt tccattccca acatgcgcag ggccgattcg ttgatgatca catggttgtg
     4141 gccgctgcgt acatcgggtg cgcggtaggg ggcgccggcc agaaaacgga tgccgtaagt
     4201 ggcgtaaaaa tctggttcgg cattgacaaa ataagtagtg ccgggcctca catcgtcggg
     4261 gcgatgcgcc ggcaccattc cgccgctgga accggaaatg ttgttgattc ccaagcccgg
     4321 tgttatgctc gacgccgcca cttgccggat accgctgatc tggcggattt catttttaaa
     4381 aaccgccatg ccgcgacgat tgaggctgtc ttcccgccag tcgtaagccg agtttctgac
     4441 cgccaggatt tgatctaaag acaaaccttt gtcgtgccga tgcaaataat tcaactgccg
     4501 gcttaccacc atcacagaaa aaataagacc gacggtaaag ccaaactgaa tgactaccaa
     4561 tgacttgcgc aaccagctcc ctttggaatc tgccgaaaca agcggtattg ccccgctcat
     4621 caccgacagc gggctgaaac gggtgagcgt ccaggccgga taggcgcagg aggccaatac
     4681 gcccaccata aagatgccgg ccaccgcgcc gctaaacgtc atatcaagcc gcgcatcggc
     4741 caagggacgg cccatcaact gcccgaatgc aggcagcgcc atttgaaaaa tgccgagcgc
     4801 cgtcaacatg gctccggcat tgagcagcaa gccctctacc aaaaattgcg agaataatct
     4861 acccctgccg gcgcctacca ctttgcgcag gcccacctct ttgctgcgct ccaatgaacg
     4921 cgccgtcatc atgttcacat agttgataca ggcgatggcg agcacaaaaa gagctgccgc
     4981 caacaaaaaa ccgaccgtgc gggcgctgcc gttgggttca atttcataag tcatgttcga
     5041 ctggaggtgg atgtcttcaa aggcctgcat attcagccgg atacctgcat cactgaggtg
     5101 ctgttgggaa tacacattca attggcggcg cactttttcg gggtcggcgc cgggttttag
     5161 ctggaaataa ttgtagtccc aatagccggt ccagttgtcc tcatgcccgc cggcataccg
     5221 ggtggcgtac gataccaacg cgctgaattt caggtgtgta ttttgaggtg ggtcgttcac
     5281 gacggcagag acgacatact cgccccgcag ttgtccgcga ggaatccgca gcgtttgccc
     5341 catggcctgc ccctccggaa acaggcgggc agcggcgctc cggctcaaga ggatttggcc
     5401 gggttgagaa agacaggaat tcgcatttcc ggcaacgaaa tcctgaggaa acatgcgcag
     5461 aaagcccgga tcggccatcc atacatgcgg cagtcgaacc ggctcgttgt tttggaaaaa
     5521 aaccgtctca tgctggttgc tgttgaagag cctgaaaaaa tcggccactt cgggcatatc
     5581 ggtttttaaa gccggaccaa gcgcagaatg agtgttgcaa tcctgcgtag cgacgcgccc
     5641 ttcgctgagc gtttcgttga aggcgcgcca gattagatcg gttccgggcg caattttgtc
     5701 atagctgcgc tcataagcca catatttcag gatgagcagc gcagccgcca tgccgatgct
     5761 gagtccgaat aaattgatga aggtaaaact cctgtgcctc agtaagttgc gccaggcnnn
     5821 nnnnnnnnnn nnnnnnnnnn gatactgcca catgagaaag gtgttttaca aggttagatt
     5881 acagattact attcagcgcc ctgctaaaag ccatttgcat agcgattgta ttgttacaca
     5941 ctcactgctt tttcagccac tccaaagccc gttgaatcat ctgatcttta gtgatttgat
     6001 aaaaattttc gccgccatca cagcgctcgt cgggcaagac aggcgtgccg ggaaacacat
     6061 tttcgaggcg gtcggcatag tagctttcgg tcagttgcaa taccacttgg tctgagatgg
     6121 gctgccagga aacggcggtg gtgtagcctg ctgtatcttc tccgaaaaag cgggtgcgcg
     6181 ggcggtttcg gaatgctaag gctgtcacct cgcccgaact ggccgttgcc gcgctgatca
     6241 tcaccgctac cggcaagctg gagagcgaac ggggaatgcc cattttttgc cacaatccgt
     6301 aatccgtcac ttgatattca ccaacaaaaa cctcgccctg ccgcagcgtc caggcttctt
     6361 tgtagccggc atcgggatgt ataaaagagc cgatttctgt gctgtcgccg agcaaggcgc
     6421 ccagcccggc cagcatggga aacatggtgc cgccgccgtt gaggcgcagg tccacgatcc
     6481 agccgcgcag ttttacgccc ttttgcaccc cttccatcgc ctgccggatg atgcgggcgt
     6541 tgatagaatc ccgctctccg aaagtgatgc ccggcacccg aatgtagccg atgcggccgg
     6601 ggaggcgttg ggcaaaaaca ggcagttcgt ttcggaatag tttatgcaat aagctggtat
     6661 ccaattgcat agaggtgggc gtccagggtt tcactgctcc ttggtgcggc acattgtcca
     6721 cccagatttt gccgtgaaaa tcgcggagtt cgtgcaggag ccgctttgtc gccgggatga
     6781 gaccttcgga ggcgcaaacg gagtccagcg tgggtcggag cgccgcccaa ttcacgctgt
     6841 cgcgcaatac ggaatggccc tgtgcatagt tgaaaatgct gtcccattcg gggccgccgc
     6901 cgggagcagg ctgtgcctcg gcggtagcga ggaagagcat aaaaagcagt ggaagtggtg
     6961 tgtatttcat tgtattttgg ttgtgaagcg gaagaagtgc caccgacttt agcctccgaa
     7021 acgatacggt agccgccgaa aaaaacgcag taccgccgga gtgaaaatac agcgcaagtt
     7081 ttgatcggcg actgaagtca ccgatgcctc acggcggcta aagccgcagc tactcatttt
     7141 tcaagctctc caccgggttg gccagcgcgg cgcgtacggt ttgcagggcg actacaaggg
     7201 ttgccccgac gagggccgcc gcgcctgcca gcgcgaaaat ccaccaggac agttcgatgc
     7261 ggtaggcata aaattgcaac catttttgca gcaaaaagta cgcaaccggc gtagagagca
     7321 caaagcctgc cagcaccagc aacacaaact cccggctgag catgcccacc accgaaacag
     7381 ccgatgcgcc cagtactttg cgaatgccga tttctttggt gcgcttccat gccataaagg
     7441 tggccagccc aaagaggccc agaaaagaga tgaaaatggc gatgccggtg gccaattgaa
     7501 tgaacttggc cagcgagagg tcttgctcgt aaatatcagc taaggtctca tcgtaaaatc
     7561 tggcggcaaa cggctcatcg gcataaatgc gcttccattc cgtttccatt tttttcagca
     7621 cgatctgcca gtcggatggg ttgctgcttg ccaaccgcac attaagctca cccacagatt
     7681 cgagatcgcg catcatggcc gtagggcgaa tcgcttctgt gaatcccata gagtgaaaat
     7741 ccgctgtcac gcccacaatt tgtttttgcc ggggcggttc atcggggccg ttgttttcct
     7801 gaataaaact gcccactgcg gcctgcgggc tttcaaaccc aaaagccttc accgccgctt
     7861 cattgatgac gtagtgttcg gggccgtcgg aagcctgcaa tttgcgcccg gccaagagcg
     7921 gcaatttgta cagatccatc agctcctcgt cggcattttt ccgatagata ttggtttctt
     7981 ttttgttgcc ggcctcgtcg gtgtagtggt gcgtattgct cgaatagctc atgctcagca
     8041 gggcatcgcc gagtgcaacg cgctnnnnnn nnnnnaggca tttttttgaa ggcatcggcc
     8101 aacaccggaa atttgttttt cagatcggga ttgcgccagg cgctcatggg ttgctctatg
     8161 gtcagcacgg cctgccggtc gaatccgagg tcgctgttgc gcataaaatg caattgctgc
     8221 cccacgacca aggctccgat gatgaaagcc tgtgccacaa aaaactgaaa cacgatgagg
     8281 ccccgtcgca ggttgccgcc gcgcttccga ctgccctgct cctgccccga aaactgcccc
     8341 cgcaacaaag ccgccgcctg aaaccgcgag gcaatccaac ccggatacag ccctgaaaac
     8401 aaggtgaccg cccccaccaa tacggccaaa aacgcgccgg ttttacgcca gtcggcatag
     8461 cgcagtacgt cttcgggcag gtactcgctg aaggcgtcga aggcgaaacg ggtaagtccg
     8521 ccggccagtg ccgttgcgag caggcacacc aacagggttt ccagcaggaa gctgccgatg
     8581 atgctcctgc ggctgccgcc gaggggtttt cggatgccga tttcgcgggc gcgggcagga
     8641 atttgtgccg tgctgaggtt gacgtaattg ataacggcca gcagaagcag gaagatggcc
     8701 actgctgcca atatcttgat tatcttggca ttggctaccc ggatatggct gccgtatcgg
     8761 tcggcaaaat gcacgtcggc caaagcctga agctgatgcg ttcggctcat gccccattgt
     8821 ttgagggctt cgccgccttt ggtttgcgac acctgattga tggcggtggt gatggcctcg
     8881 cggtcggcat ctgggttcaa tatgacccag agttggtcgt tggaattggt gccgcgccac
     8941 ctttcctcgt caggttcggt caccgtcagc agttcttttt cgatgaagct ggtgggaaag
     9001 tccaaatctt ctatcacgcc cgctacggtg acggtcagtg tgtcgttgaa agactcgtac
     9061 cgaatcgttt tgccgagtat ttcttcgggg ctgtggcgcg ggaagtactt ctctgcgcgg
     9121 ctgcgggcca gcaccacttc atcgggttgg ctgagtgctg tggcgggcga acctgctaac
     9181 cattttacgg gtacgagctc gaaatattcg ggcgtggttt tggccatttc acgcacttct
     9241 ttgaaacccc gtttggcgcc ttccggcaat gccagcaatg cgaaatgcga ctgtatgggc
     9301 actgattttt cgatgccggc cacttcctcg gcggcgcgcc agagcggttg cggacagcct
     9361 gcattatgag agttgatgtt gtcggaatca ttaaactcag agaccacccg gaaaatgttt
     9421 ttggcattgg gaatgaggcg gttgtggctg tattcaaagt cggcgtattg ccagatcagc
     9481 caggcggcgc tcagtccgat ggccagcccg gcaatgtgca gcagggtgaa caggcggccc
     9541 cgccagagga ttctgaaagt tgattttaat tgattgcttg tcatggtatt gtgtgttttg
     9601 tgacggcgat tttagccgcc gaaaaaacgc gaaaaaacgc agcgcaagtt ttggtcggag
     9661 actgaagccg ccggtactac tcgcttctca aactcttcac cggattggcc agcgcggcgc
     9721 gtacggtctg cagggcgacc accagggttg ccccgacgac tgccattacg cccgcagcaa
     9781 cgaataccca ccagggcagc ccgattcgat actcaaattt ttccaaccag tctttgagaa
     9841 accaggccgc tgtcggggca gcaacaaggg cagaaacgag taccaaaccc acaaattctt
     9901 tggacaacaa agcccacaag ttggaaaccg aagcccccaa aaccttgcga atacctattt
     9961 ccttcgtgcg ccgttctgcc atgtaagccg acaggccgaa caatccgagg caggaaataa
    10021 aaatggccag tccggcaaaa atgcctgcta attttccaat cagctcctcg gtggcaaact
    10081 tgcggctgtg aaattcatcg gcaaaagtat agtcaaaagg ataagccgga ttgtgcttct
    10141 tgaatatggg ttccaactgt gccagtgcct gccgggtatc catttccggg ttcagccgga
    10201 acatcatgtc attggcccac aatgggttgg acaaaaacat ggcggggctt actttttcat
    10261 aagggttcgt cattaccaca tcgttcacca cgccgacgat gcgaaaagtc atgccctgcc
    10321 attgcaccgt ctcttccagc gggttttcga ggcccatgcg ttgaatggcg ctttcgttga
    10381 aaatgatggc cgtgctgtcc gatttgaaat cggaagagaa aaaacggccc tctttgagcg
    10441 tcatgccgag cgtggtaaag tagtcttcag cggtgggaat ggtcgcaaaa atgatctcat
    10501 cactggggtc tttgccgctc cattccacgc ctttgttgtt ggaataaatg ttcgtcacgg
    10561 ggctgccggc tttggtcacg ccggtgacga ggccggaggc gagcatctca tttttcagcg
    10621 gcagatagtt gtctaaaagg tcgccgctca tcaccaccat taccacgcgg tcggggtcgt
    10681 atcctaatgg gcgatttttg gcgtgccgta tttgctgaga taccaccagt gtgctgacaa
    10741 tcaatgcaat ggagactgaa aactgagaaa cgaccaatac ttttcttggc aataatgctg
    10801 ccttgcccag gccggaaaat gttcctttca atactttgat gggttggaat gaggacagaa
    10861 aaacagccgg gtaactgccc gccaacaggc ccgccaaagt ggtgaatccg agggtcaatg
    10921 cccaaaaaac cggactgccg taaggcgtag aaagctcttt tttcgtcagc agattgaagc
    10981 ccggcagcac cagttccaac aataacaacg acaaaatcat ggcaatgccc gccatcagca
    11041 gcgcctcgcc gaggaattgc ccgatcaact gcccgcgagc cgagccgagg gtttttcgta
    11101 cgcctacttc tttggaacgg cgctccgaac gagccgttga gaggttcata aaattcacac
    11161 aggcaatgag caaaacgaac atcccgatga tgccgaacag ccgcacatac tcgatgtagc
    11221 cgcccgccac ccggccgttt ttgaactcgc tgcgcaaacg ccagtcggcc aaagcatgca
    11281 gggtcacgtc ggctttactt tccttgttgt ttcggcgaat gatgtctttg acttttgcag
    11341 caaatgcagt gccgtccacg ccgggctgaa gctccataaa catttggaaa gagttgttgc
    11401 cccagtcgtc gcgggagtct ttcacccaat ccatgtcttc ataaagagag aacggcaaga
    11461 ggtagttgaa gctcaggctg gagttgcagg gcacattttc cagtacgccg gttacttttt
    11521 ggtcgtaata attgtccacc cgcactacct cgccgagcat gtccgaccag tttttgtgcc
    11581 caaacagaga ctccgccatt tctttggtca aaacgatgga attgggcgca tcaagggctg
    11641 tttctacact gccggccacg agcggaaaac tgaacatttt caaaaaatcg tcgtccacct
    11701 gaaagccgct tttgaaaaac agtttgtctt cgtatttcaa accgtgttgc cagccccaat
    11761 cggcctcggc tacatacctg acttcgggaa atgacttgcg cagttcttcg gccatagcca
    11821 gcggcaccga actccaggtt tccaattcgc cattcacata gtcgctcatt ttgacctgat
    11881 acaggcgctc gcctttttca tgaaactggt cgaagctcag ttcgttggca atccacaggc
    11941 caatgagcag ggcaaccgtc aggccgatgc cgaggcccgc aatattgagc aggctgaaac
    12001 ctttgttgcg ccaaagggtg cgcagtgcga cttttaaatt gtattgaaac atggtatttt
    12061 ggttttgtat agagtaccgg cgactttagc tgccgaaaaa aaatcagtgc cgccggagca
    12121 aaaatgcagc gcaggttttg atcggcgact gaagtcaccc tactcgcttc tcaaactctt
    12181 taccggattg gccagcgcgg cttttacggc ttgcagggcg accacgaggg ttgccgcgac
    12241 gaccgacaac gcgcctgcca cgacgaacac ccaccactga atatcaatgc gatacgcgaa
    12301 attcagcatc catttgctca tcagatacca ggcaaaaggc gaggcgatga acagcgcaat
    12361 gagtaccaag accaggaaat ctttcgacac caaagcggtt atgcccgccg ccgatgcgcc
    12421 cagcactttg cgtatgccta tttctttggt gcgctgcgct atgtggtacg atgccaacgc
    12481 aaacaaaccc agacaagcta aggccactgc cagccaggaa aagacgccga tgatgcgcgt
    12541 ggtctgcatt tcggtctggt acatttgctc cnnnnnnnnn ntcttcatcc aaaaaatgga
    12601 agctgtatgg ccggtgcggc gctacccctg cccacagggc gcccatgcgt tcaatgcttt
    12661 gagctacatc gccgcctttc agttttacaa aaatgtactg cccccagcca tcagggaaaa
    12721 tgaccaatgg cgccaccgct tcgtgcaaag accgaaagtg aaaatcgcgc accacgccct
    12781 tcaccattgc cggagcacca ttgaaataca ggcgtttaga cacagtttct tccaaagacc
    12841 agccaaacgc cgccacctgc gcctcgttga gcaaaatggg caatacggcc gtagaatcat
    12901 tgcgcagtcg ggaggctgta gccagatcgg cctcgttgaa atcgctgccg gccacgagtt
    12961 cgatgctcat ggtttttaaa aaatccagac cggcgggcag ggccgtcacc gggcgcgcat
    13021 cctgctgcaa cagggtaggg ctgatgtcat agccgccttt gatgttggag ggcgtgccgt
    13081 agctcatcgt caccgattgc acctccgaaa gttggagcag ttgtgatttc aaagccccgt
    13141 attggcccac cacctctccg tcggcgggca gcaccagcat ttgttcctga tcgaagccca
    13201 attttttatt ttggataaac tgcatctgct cgcgcagcaa caaggtgcag ataatgagca
    13261 aaatagaaat ggaaaactgc gcaatcacca atgtttttcg cagccaggcg cccgatttgg
    13321 cattgcccgc ccagcccttc agtaccgccg ccggcgagaa ccgggccgct accaccgccg
    13381 ggtaaaaccc tgccccgaaa ctgaccgcca accccagcac cgccaatgcc acccaggccg
    13441 caggctggaa aagcggctcc gctgtgagtg cccggttgaa aattcggtta aaaaccggca
    13501 gcagcggagc cgtggtcaaa aacgcaataa ataatgccaa aaatgtaacg atggcagcct
    13561 cgccgaggtg ttgagcggcc aattgccggc tgccggcgcc cagcactttg tgtacgccgg
    13621 tcactttggc gcgctccatg ccggtagctg tggcaaggtt gatgtaggtg gtacagccga
    13681 tgagcaaaat cagcagggct gccacgccca atatgtagat gtaacggacg tcgccgttgg
    13741 gttcaaaatc gccgggcaca gtagagcgca ggtgtagatc gcgaagtgga agcaggtgca
    13801 atttgaggta actgctgccg tcgtcagcgc cggtttcgtg ggctttggag gccatatagt
    13861 cgggcaggcg ggcttgcatg agggcctgtc cttcggggtt tttgagcaat aaataagtgg
    13921 cgtagttggc gttccaccag gtttcatttt cggggcgggc tgccgacaaa cccacaaacg
    13981 aacacacaaa atccggcttg atgtgtgtat tggcgggcgc gtcggccatt acgccggtca
    14041 ctttggcttc ccgttttcca atgttcagtg ttttgcctat cgggtcggcc tcgccgaaat
    14101 agcgtcgcgc cgtgctttcg ctcaatacca cttgccaggg cgcatggagg gctgtggaag
    14161 catcgccttt caggagtggg aaactgaaaa tctgaaaaaa cgtggaatcg gcgtaaaaaa
    14221 actgtgcctc ttcagtgagc atggcgcccg attttaccac ctcggtgtat tgcatcacgc
    14281 gcacggcggc ctccacttcc ggaaactcgc gggccagcgt gggcgctact ttgttgccgg
    14341 tactgcctga atgcaccacc tctccgccga agctgtactc catgttcacc ttcacaatgc
    14401 ggtcggcatg ggcgtgcatg gcgtcgtagg tccattcgtg atgaatgaac aggccgatga
    14461 gcaggcaggc tgagataccg gctgtcaatc cgaccaactg cacggcgctg aacaaacggt
    14521 tgttgagcag gcgcctccag gcgagtttga gatggttttg gaacatgagc ctttgatttt
    14581 caacaatatg agaacaggct cttctccata cgaatcgcat gatcttctgt ggcaatacag
    14641 agtgactgaa cgcatcagcc ggcccaggtc cagatgagct ttttcatttt cactttcagg
    14701 attatttcaa tttcattgtc tttcacaggt tgtaccaccg cttctttttt tccggaaaac
    14761 cgcatcatga tttttccgtt tttgccttgt ttccaaatac cttccctgat acctgcaatc
    14821 ggctcgtcgg cagcccattg accgtttgta aaaaactcta actgagtgtg tttgggcaag
    14881 gattgatggg tgcccattcc agacatcaca tcttctttca atgaccattt ccggtctgtc
    14941 agccaaaacg gcaactctgt ttgagcagat gctccggcaa ccatcaacag gaagagtgcc
    15001 ggcaaaatca ttcgtttcat tttttcaaaa tattggatga aaaaacaagg ttatttctga
    15061 tgctaaggaa tgagtaaaga ataagttccg cattttggcc gagcagattt tgtactcaga
    15121 cgaggaaaaa aacacagcca atgtggggca ttggcgaggc tttttgagga agtatgagtg
    15181 caaaagatgc cgccataaat tgagaagtta ttctttaatc attcctaagt agtggggcaa
    15241 aaataaatgt tcattttctt taggccaaag gattagata
//
LOCUS       DAORLF010000048        14994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2131_length_14994_cov_2.364148, whole genome shotgun sequence.
ACCESSION   DAORLF010000048 DAORLF010000000
VERSION     DAORLF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2131_length_14994_cov_2.364148"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="PK198_06285"
     CDS             <1..1927
                     /locus_tag="PK198_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRF38374.1"
                     /translation="HWFQPLTGTTAEKHDAFFEPVGDGTAIDKFDGGQLVQQEPDASS
                     FPSGGIRNTFEARGYTAWDPTSPAFVMGTTLCIPTVFVSYTGEALDNKTPLLRALQSV
                     DQAATEVARYFDKNVNKVISTLGWEQEYFLVDTALAASRPDLVMSGRLLLGHSAAKGQ
                     QLEDHYFGSIPDRALAFMRELEIECIQLGIPVKTRHNEVAPNQFEVAPLFEEANLAVD
                     HNSLLMDVMAKVAARHHFKVLLHEKPIAGINGSGKHNNWSLATDTGVNLLSPGKTPKS
                     NLMFLTFFINTIKAVHDYADLLRASIASAGNDHRLGANEAPPAIISAFIGQQLTAVLD
                     ELENVTDGNLSPEEKTELKLNVVGKIPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSA
                     NCAKPMMVLNTIMAETLRKFKKDVDALIEGKGLKKDDAIFNVLREYIKASKKVRFEGD
                     GYSDEWVEEAEKRGLSNFKTTPEALKVQVAKETIRLFETHQVMNKIEVEARYEIELEE
                     YIKRVQIEGRILGDIVTNHVIPTAIAYQNTLIQNVRGLKAIFESDFEQYAVQQIELIK
                     EISGHIGKIKTFVDEMVEERKKANVIAHAEERAEAYCHHVKPYFDQIKYHCDKLELLV
                     DDELWPLTKTRELLFTR"
     gene            complement(1992..2630)
                     /locus_tag="PK198_06290"
     CDS             complement(1992..2630)
                     /locus_tag="PK198_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF38375.1"
                     /translation="MAQIVQLIFNDFSENTYLIFDDSGECVVIDPGCNNSRERNELAG
                     EIARRQLRPVRLLNTHCHLDHVFGNAFVANAYSLPLEIHRDDLPILKAVPQVCRMYGL
                     TEPEPSPQPGRFMEEGEVIEFGHTRLEVIHTPGHSPGSVSFFCREDRFLISGDVLFYG
                     SIGRTDFPGGHHATLLSSIENKLFPLGDDVQVYSGHGQATNIGFERVHNPFL"
     gene            2753..3046
                     /locus_tag="PK198_06295"
     CDS             2753..3046
                     /locus_tag="PK198_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38376.1"
                     /translation="MTASIEKKYKLIQLIMAEDDDHLLTQIEDSLTSQKMQVQEPLPS
                     FFKAVIPARKGLSFKKILEEQKYQPLTYEEFREKAADVDIQEPLDELLEMLTK"
     gene            3059..3520
                     /locus_tag="PK198_06300"
     CDS             3059..3520
                     /locus_tag="PK198_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRF38377.1"
                     /translation="MNYLLDTNIVLIYTRDSSISKKMEEELKIFTPENNVAISVVTVG
                     ELFSIAKQKQYSDHRIRRRRALLSSLAVIDINIEEIIEQYAEIDAYSQARHSTLQSGF
                     TARNMGKNDLWIAATASIYDLTLLTTDQDFRHLDNVFLRLRQVDLSLYKDR"
     gene            3552..6968
                     /locus_tag="PK198_06305"
     CDS             3552..6968
                     /locus_tag="PK198_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38378.1"
                     /translation="MRILLIFTILMLVAASASAQGAQVEFGKNRVQYHRDFDEWSEYE
                     SEHFITYWYGEGRNIGQAAVQIAEQEFLAVQGIMEHRINNKLQLVIYVDLTDIKQSNV
                     GSEEAFTNTGGQTKIVGNKIFVYFDGDHNHLRRQIREGIASVFLEAMLFGASLQEIVQ
                     NAVMLSLPEWFKQGLVAYVGESWNPELDNELRDALLREDFPGFERFAAENPRLAGHAM
                     WYYIGSNFGNNSVSNLLYLTRINRSVESGYQYVLGSPYKEVLSVWERYFRRRYAQDAT
                     NRDTPNRGALKIKNRRNAPITHISMHPNGRSVAYVINELGRFSVWTQEARSDERQRLF
                     RHGSRNAFQTTDYNYPLIAWNPNGKELAVIYERRDVIKLLRINTENKKTEVNDITSPF
                     QRIHSLDYSSPTSLIFTATVRGFSDVYQYFIPTTQSERITGDIYDDLDAAFVRIGQQR
                     GVLFSSNRPDSLLAPVRLDSVLPLGRFDLFFYDLDRRSNELVRITSTPDADEFSPVAV
                     DSARFAYISTRSGMANREVAFLEEYIHHYDQILQLKDGSEIILHADSAWIGIDSAAID
                     SVIIRPVVKQRAVAHANTNYDRSLLKQSASLRAGRIAELMLRERRHRIFVRDMSPDSV
                     VQTAPTWFRQPPLSAAPPAADKTAPASATLPALPAPPPAAPDTGKLNIDTYLFQTEFD
                     PAEKPVVTVKPADAETTEDIRLFVPPPAAAPAARRSEMHRFRPGLITASRLQFHTDFV
                     TTQMDNSILFEGLESFSLNPQRFTYPVPGMMFKANFKDLFEDYELEGGLRLPTTFNGI
                     EYYVTYQNKKRRLDQRFSVYRRNTRMSTEGAILPSRKELNTILGQYGVRYPLDLFTSL
                     RATATLRRDRLTQLATDLPTLNTPTRAEQRIGLKLEYVFDNTIDVATNIKNGTRYKFY
                     ADFYKAFNFQLANGTAFEFDKGFLGVLGFDARHYIRLGKYPVLALRAAGATSFGSQRF
                     LYYLGGVDNWLLPRFDNSVPIAQPGSEFAFQAYAGPMRGFDLNIRNGNSYAVVNTELR
                     LPVFRMLSRRIGSNFLRNFQMVGFFDVGTAWEGANPFSTDNPLNSSVITNGNRVIVKI
                     NYFREPVVAGYGVGIRSMLFGYFVRADYAWGIETRTVQRPKLFLALGLDF"
     gene            complement(6970..>7289)
                     /locus_tag="PK198_06310"
     CDS             complement(6970..>7289)
                     /locus_tag="PK198_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38379.1"
                     /translation="SPDGKRLLSGAEDKTAHIFEFPSGKLAQTLQGHAQRIWSVAWSP
                     DGSRVATASADLSTCLWDARTGQRTLLLLGRQPVYNLLFTPDGRLLANEMNGKVQVWE
                     AGE"
     assembly_gap    7290..7358
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7359..9034)
                     /locus_tag="PK198_06315"
     CDS             complement(<7359..9034)
                     /locus_tag="PK198_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38380.1"
                     /translation="MQFLFHPARNISVQFILFLLLATPLVAQKKAAQEYQSYLAHIAA
                     ANSAILLNRSPEARQWLETAPVKHRGWEWHFLNKYSNQSTGTYEISGEQPANFSLSPD
                     GRSVALAMPGGSVEIRDAQSMETRLRLEGHSKAVYSVAFSPDGSSIVSVSRDSSIRAW
                     DTGSGRQLWTASSGGHGFAVTVCSPNGSTVATASWFRDKARGVVGLIKLFDAHSGKEK
                     WSAEFGVKPTLGLAFSSSGRYLAAGNWDGQVGVWDLQQPTKAPAILDFSDRNDYTAID
                     DIAFHPTNDSILAAASKCGEPRIWNTQTGKRIAELRGHRQAVMAIAFSPDGRRIYTGG
                     DEGVLHVWNAETGNLNTQLHGHAGRVTRIRPTSDGKALLTLSDDKTIRRWTASADAAF
                     DAPRSSDTYVYAFAGSPDGTTLAMGAPNGQVSVWNAQAGTMKNQFPCLEEGANAVALN
                     PDGSQLVAVNWGAKVPILDARTGAAVRTLEGFEGGSSGCAWSADGRWIAAASRRNFVF
                     VWEAETGRLIQKINCESGTYFVQFSPDSRRLATAGNDGKILVWDMTQAEQG"
     assembly_gap    9081..9107
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9313..10437
                     /locus_tag="PK198_06320"
     CDS             9313..10437
                     /locus_tag="PK198_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF38381.1"
                     /translation="MYSSPAITMLTFALGAAALHGFWLSALCVGRAHTRWLSLALLGL
                     SLYLLNYLLFLTGIIRALPNMLGVLFPFVYLTGPALYLFISDALNTDFRFQKNHWLHL
                     LPFVWGWWQMRNVVGMPAQEKMAIIGWLLDPQALLPLRSVLLGNLHIYALLGYGFAAW
                     LLAHRAQKSAENESHQKIARWYRLFALLFIAVLLLDVAAKITFSSLSIPGSNAEYALA
                     LLPALALHAVGYYALRATGGFPKIQPEAAPAAKYKTSPLDASRLAVGKQALLQWMETE
                     RPYLNSNLKITDLARQMNMPSHHLSQILNEAIQLNFNDFINAYRISEAQRRLKDERYR
                     HLSIEALGMDCGFANKTTFNRAFKKIAGTTPGEFLKKNTE"
     gene            complement(10520..10927)
                     /locus_tag="PK198_06325"
     CDS             complement(10520..10927)
                     /locus_tag="PK198_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38382.1"
                     /translation="MKNTIISGIALAFLLSIAFSNSASGQSAMDKWPELKTFHSVMSQ
                     TFHPMEEGNFDPIRKRSGEMAEKAAALAKSAIPAEFNKPAMISAVKALKSGSKKLDKV
                     VKKGAADEVVGKQLVSLHDTFHTIVGICRGEDH"
     gene            11120..>12206
                     /locus_tag="PK198_06330"
     CDS             11120..>12206
                     /locus_tag="PK198_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HRF38383.1"
                     /translation="MKRKSLLFLCSFLSAFALGAQPLNHVYYRTIDGTANNQQNPTWG
                     AAGAQLLRFSGTAYADGISAPTGVNRPNPRAISNMIFAQGNFMPAPQGLSDYMWVWGQ
                     FIDHDFGLTPDINETLHIPIPSGDVHFDPFGTGQVSIPVHRNLFDVTTGTGIANPRQH
                     INIISSYIDASFVYGDRNARAAWLRTFTGGKLKVSEGNLLPYNTLNGEFWGEIDANAP
                     AMANPVGLSNKFFVAGDERANENPLLAGLHTLFVREHNRMCDELAKQHPDWSDERLYQ
                     HARKYVGGFVQAVTYREWLPQLGVNVPAYTGYKPNVNATLANEFTAAAFRLGHTLLNG
                     NLQRLYNNGESLEEGPVALRDAFFNPFA"
     assembly_gap    12207..12270
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12271..>12822
                     /locus_tag="PK198_06335"
     CDS             <12271..>12822
                     /locus_tag="PK198_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HRF38384.1"
                     /translation="MQQFDAKVIDDVRNFLFGPPGAGGLDLVAINILRGRERGLPDFN
                     TIRAAYGLTPYSSFPQINSSSQVFSALHELYGDINNIDPWVGFVAEQQNSPQTVVGPT
                     LQRILQVQFTALRDGDRFYYENDPVLTDEEKDIIRNTKLRDIVVRNSGLPMLQDNVFK
                     AMPHSEICDNMHVRIYGRFRSVL"
     assembly_gap    12823..12838
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12839..14024
                     /locus_tag="PK198_06340"
     CDS             <12839..14024
                     /locus_tag="PK198_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRF38385.1"
                     /translation="VQLAVQMTGNDLDGQSDAFGAYDFNDVKGCDVQGFTVSKNDPAS
                     TGVSTLDIILIQRHILGSELLDSPYKLLAADVNRSGSISTLDIVLMRRVILGIATEFP
                     DGNVWRFIPTAHSFSDPANPFADNIIEPPMHFDLMALNYNGDFVAVKAGDVNGSAVSG
                     FHEDEVELRQLAALRTLDQSFRRGEQLAIPVFCNEISGLSGIQIEWNYGSELELTDIE
                     VHGNSGLSREHFGRFATEGVLTMSWNQSDLSEALANGAWFTLHFTALKDGQLSETLRQ
                     SDTKTPSEAYTKGLETRHLLWYFDTDSNAAAAGFELYQNRPNPVADATLIPFLMPESG
                     QVRLSIVDVAGRVLFTADRHAEKGYNEWQLERAQLSASGLVLYRMQTPHGTLTRKMLL
                     AD"
     gene            complement(14117..>14994)
                     /locus_tag="PK198_06345"
     CDS             complement(14117..>14994)
                     /locus_tag="PK198_06345"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767690.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRF38386.1"
                     /translation="KSLEKLLPIQRGDFEGLFEAMHDLPVTIRLLDPPLHEFVPHEEE
                     NQRQMAADMGISLEAIKHRVEELHEFNPMLGHRGCRLGITYPEITEMQARAIIEAALN
                     VKAKGFVVRPEIMIPLVISLREFKEQEARIRKVAEQVFAERKDRIDYLVGTMVETPRA
                     AMVADSIAKGAQFFSFGTNDLTQLTMGFSRDDAGKFLPLYVKRGLLESDPFKSIDQKG
                     VGLLVDAAVKKGREINPNLHVGVCGEHGGDPASVEFFYKTGLDYVSCSPYRVPIARLA
                     AAQASLKYGKVAETV"
BASE COUNT         3382 a         4173 c         3883 g         3380 t
ORIGIN      
        1 acactggttt cagcccctca ccggcaccac cgccgagaaa cacgacgcat tttttgaacc
       61 cgtaggcgac ggcaccgcca ttgataaatt tgacggcggg cagttggtgc agcaggaacc
      121 cgacgcttcc agcttcccca gcggcggcat ccgcaatacg tttgaagcgc gcggctacac
      181 ggcctgggac cccacctcgc ccgctttcgt gatgggcacc acgctgtgca ttcccaccgt
      241 atttgtgtcc tataccggcg aggcattgga caacaaaacc ccgctgctgc gcgccctgca
      301 atcggtggac caggccgcta cggaggtggc ccgttatttt gataaaaatg tgaacaaggt
      361 catttccacc ctcggttggg agcaggaata cttcctcgtg gacactgcgc tggcggcgtc
      421 gcggcccgac ctcgtcatgt cggggcgttt gttgctgggc cactcggcag ccaaaggcca
      481 gcaattggaa gaccactatt tcggctccat ccccgaccgt gcgctggctt ttatgcgcga
      541 actcgaaatc gagtgcatcc agttgggcat tccggtcaaa acccgtcata atgaggtggc
      601 gcccaatcag ttcgaagtgg ccccgctttt tgaagaagcc aacctcgcgg tggaccacaa
      661 ctcgctgctc atggatgtga tggccaaagt agcggcccgc caccatttca aagtactgct
      721 ccacgagaag cccattgccg gcatcaacgg atcgggcaag cacaacaact ggtcgctcgc
      781 taccgacact ggcgtgaacc tgctcagccc tggcaagacg cccaagagca acctcatgtt
      841 cctcaccttt ttcatcaaca ccatcaaggc ggtacacgac tacgccgacc tgctgcgcgc
      901 ctccatcgcc tcggcgggca acgaccatcg tctcggcgcc aacgaagcgc ccccggccat
      961 catttcggcc ttcatcgggc agcaattgac ggcagtgttg gacgaattgg aaaacgtaac
     1021 cgacggcaac ctctcgcccg aagaaaaaac tgagttgaaa ctcaacgtgg taggcaagat
     1081 tcctgaaatc ctgctggaca acaccgaccg caaccgcacc tcgccctttg ccttcaccgg
     1141 caacaagttt gagttccgcg ccgtgggttc atcggccaac tgcgccaagc ccatgatggt
     1201 gctcaatacc attatggccg agactttgcg caagttcaaa aaagatgtgg acgccctcat
     1261 cgaaggtaaa ggattgaaaa aagacgacgc cattttcaac gtgctgcgcg agtacatcaa
     1321 agcctccaaa aaagtgcgtt ttgagggcga cggctacagc gacgaatggg tggaagaagc
     1381 cgaaaagcgc gggctttcca atttcaaaac cacccccgaa gccctcaagg tgcaggtggc
     1441 caaagaaacc atccggctct ttgaaacgca ccaggtgatg aacaaaatag aggtggaggc
     1501 ccgctacgaa atcgaactgg aagagtacat caagcgcgta cagattgaag gccgcatttt
     1561 gggcgacatc gttaccaacc acgtcattcc caccgccatt gcttaccaga acacgctcat
     1621 ccagaacgtg cgcggattga aggcaatctt cgagagcgat tttgagcagt atgctgttca
     1681 gcaaatcgag cttatcaagg agatttccgg ccacatcggc aagatcaaaa cctttgtgga
     1741 cgaaatggta gaggagcgca aaaaggccaa tgtgatcgca catgccgagg agcgcgccga
     1801 agcctactgc caccatgtaa aaccctactt cgatcagatc aaataccact gcgacaagct
     1861 ggagctgctg gtggacgacg agttgtggcc gctgaccaaa acgcgggaac tactatttac
     1921 gcgttgatcg cgctgttttt ctaaaggaat atttcccccg ttcggcgagt gtgccgggcg
     1981 ggggattttt tttacaaaaa cggattgtgt accctttcaa aaccgatgtt cgtagcctgg
     2041 ccgtgcccgg aatacacctg cacgtcgtcg ccaagcggga agagcttgtt ttcgatgctg
     2101 gaaagaagcg tggcgtggtg gccgccgggg aaatcggtgc gcccgatgct gccgtaaaac
     2161 agcacgtcgc cggagatgag aaagcggtct tcgcgacaaa aaaacgaaac actgccgggc
     2221 gagtggccgg gcgtgtgaat gacctctaag cgggtatgcc caaattcgat gacttcgccc
     2281 tcctccataa atcggcccgg ctgaggcgag ggttccggtt cggtcaggcc gtacatgcgg
     2341 cacacctgcg gcacggcctt gaggatgggc agatcgtcgc ggtgaatttc gagcggcagg
     2401 ctgtaagcgt tggccacaaa ggcattgccg aaaacgtgat cgaggtggca atgggtattc
     2461 agcagccgca cggggcgcag ttggcggcgg gcaatttcac cggccagttc gttgcgttcg
     2521 cgggagttgt tgcagcccgg gtcaatgacg acgcactcgc cggagtcgtc gaagatcaaa
     2581 tacgtgtttt cagagaagtc gttgaaaatc aattgaacaa tctgcgccat aattttttct
     2641 ttttgaagac ggtagttccc gcgcaaagat attatcttcg ggcaggaaag gggcgatgcc
     2701 ggtgcaaaaa ggcggcacat cagcaatatt catcattaaa tttgaaggaa aaatgacagc
     2761 ttccattgaa aaaaaataca aattgatcca gttgataatg gcagaagacg acgatcatct
     2821 cctgacacaa atagaggatt ctcttacatc tcaaaaaatg caggttcaag agccgctacc
     2881 ttcttttttt aaagcggtga ttccggcccg taaggggctg agttttaaga aaatactcga
     2941 agaacaaaag tatcagcctc tcacttatga ggagtttaga gaaaaggctg ccgatgttga
     3001 tattcaggag cctttagatg aattgttgga aatgttgact aagtaatcct ctttgattat
     3061 gaactatctt ttagatacga atatcgtact catctacacc agagactctt ctatctcaaa
     3121 aaagatggaa gaagaattaa aaatttttac tcctgaaaat aatgtcgcca tttcagtggt
     3181 aaccgtgggt gaactatttt ctatcgccaa gcaaaaacag tacagtgatc atcgtattag
     3241 aagacggaga gcactactat ccagtcttgc tgttattgac attaatattg aggagattat
     3301 tgaacaatat gctgaaattg acgcatatag ccaagccagg cactcaactt tgcaatcagg
     3361 atttacagca agaaacatgg gtaaaaatga cttgtggatt gcagccactg caagtatcta
     3421 cgatctcaca ttactcacga ctgaccaaga ctttcgccac cttgataatg tctttctccg
     3481 gctaagacag gtagatttaa gcctttataa ggataggtaa tattctgttt ttcaatccaa
     3541 taacatcctc catgcgcata cttctcatct ttacaatttt gatgttggtt gccgcctccg
     3601 cctccgccca gggcgcccag gtcgaattcg gcaaaaaccg cgtgcaatac caccgcgact
     3661 tcgacgagtg gagcgaatac gaaagcgagc acttcatcac ctactggtac ggcgagggcc
     3721 gcaacatagg ccaggctgcc gtgcaaattg ccgagcagga gtttctggcg gtgcaaggca
     3781 tcatggagca ccgcatcaac aataagcttc agttggtcat ctatgtggac ctcaccgaca
     3841 tcaagcagag caacgtgggc agcgaggagg cattcaccaa cacgggcggc cagaccaaaa
     3901 tcgtcggcaa taaaattttc gtctatttcg acggcgacca caaccacctc cgccgccaga
     3961 tacgcgaggg tattgcctct gtctttctgg aggccatgct gtttggggcc agcttgcagg
     4021 aaattgtaca aaacgccgtc atgctcagtc tgccggagtg gttcaaacag ggcttagtag
     4081 cctatgtggg cgaaagctgg aaccccgaac tcgacaacga actccgcgat gctttgctgc
     4141 gcgaggactt ccccggcttc gagcggttcg ccgccgaaaa cccgcgcctt gccggccatg
     4201 ccatgtggta ttacataggc agcaatttcg gcaataattc cgtatccaat ttgctctacc
     4261 tcacccgcat caaccgcagc gtggaaagcg gctaccaata cgtcctcggt agtccttata
     4321 aagaagtgct gagcgtgtgg gagcgctact ttcgccgccg ctacgctcag gatgcgacca
     4381 accgggatac gcccaatcgc ggcgcgctca agatcaaaaa ccgccgcaat gcgcccatta
     4441 cgcacatcag tatgcacccc aacgggcgca gcgtggctta tgtaatcaat gaactgggcc
     4501 gcttttcggt gtggacgcag gaagcccgca gcgatgagcg ccaacgcctg ttccggcacg
     4561 gctcccgcaa tgcttttcag accaccgact acaactaccc gctcattgcc tggaacccca
     4621 acggcaagga actggccgtc atttacgagc ggagggatgt catcaagttg ctgagaatca
     4681 atacggaaaa taaaaaaacg gaggtcaacg acatcacctc tcctttccag cgcatccaca
     4741 gtttggatta cagttcgcct acctcgctga tcttcaccgc caccgtgcgc ggcttctccg
     4801 atgtgtacca atatttcatt cccaccaccc aatcggagcg catcaccggc gatatttacg
     4861 atgatttgga cgccgcgttc gtgcgcatcg ggcagcagcg cggggtgctg ttttcgtcca
     4921 atcggcccga ttcgctgctg gcgcccgttc gccttgattc ggtgctgccg ctgggccgtt
     4981 tcgatctatt tttttatgat ctggatcgcc gttccaacga gttggtgcgc attaccagca
     5041 cacccgatgc cgacgagttc agccccgtag ccgttgattc tgcccgcttt gcgtacatca
     5101 gtacccgcag cggcatggcc aaccgggaag tggctttttt ggaagaatac atccaccact
     5161 acgaccagat attgcaactc aaagacggct cagaaatcat cctgcacgcc gattccgcct
     5221 ggatcggcat tgacagcgcc gcaatagatt cggtcatcat caggccggta gtgaagcagc
     5281 gcgccgtggc acatgccaat acgaattacg accgcagcct gctgaaacaa agcgcctcgc
     5341 tgcgtgccgg ccgcatagca gaactcatgc tgcgcgagcg ccgccaccgc atttttgtgc
     5401 gcgatatgtc gcccgattca gtagttcaga ccgcgcccac ctggttcagg caaccgcctt
     5461 tatcggcggc tccgcctgcc gccgacaaaa ccgcgcccgc ctcggctaca ctgcctgcat
     5521 tgcccgcgcc gccgcccgcc gcacccgata ccggcaagct caatattgac acttacctgt
     5581 ttcagacgga atttgaccct gctgaaaaac cggtcgtcac cgtaaagccc gccgatgccg
     5641 aaacgacgga agacattcgt ctgtttgtgc cgccgcctgc cgccgcgccc gccgcccgcc
     5701 gctccgaaat gcaccgcttc cggccgggcc tcatcaccgc gtcgaggttg cagtttcaca
     5761 cggattttgt gaccacacaa atggacaaca gcattctttt tgagggtttg gagagtttca
     5821 gcctcaatcc gcagcgcttc acctaccctg tgccgggcat gatgttcaag gccaatttta
     5881 aagacctctt tgaggattat gaactcgaag gcggcctgcg cctgcccacc accttcaacg
     5941 gaatagagta ctatgtgacc taccaaaaca aaaaacgccg tttggatcag cgtttctcgg
     6001 tctatcgccg caacacgcgc atgtccaccg agggcgccat cctgcccagc cgcaaagaac
     6061 tcaacacgat actcggccag tacggcgttc ggtatccgct cgatttgttc accagcctgc
     6121 gcgccaccgc cacgctgcgc cgcgaccgcc tcacgcaact cgccaccgac ctgcccaccc
     6181 tgaacacgcc cacccgcgcg gagcagcgca tcgggctgaa gttggaatac gttttcgaca
     6241 acaccattga cgtggccacc aacatcaaaa acggcacgcg ctacaagttc tacgccgatt
     6301 tttacaaagc cttcaacttc caactcgcca acgggactgc cttcgagttc gacaagggct
     6361 tcctcggcgt actcggtttc gatgcgcggc actacatccg cctcggcaag taccccgtgc
     6421 tggccctgcg cgcagcgggc gccacctcgt ttggttcgca gcgcttcctg tactacctcg
     6481 gcggcgtgga caactggctg ctgccccgct tcgacaacag cgtgccgata gcgcaacccg
     6541 gctcggagtt tgcgtttcag gcgtatgccg gccccatgcg cggcttcgat ctcaacattc
     6601 gcaacggcaa ttcctacgcc gtcgtcaata ccgagttgcg actgcccgtg ttccgcatgt
     6661 tgtcgcgccg catcggctcc aatttcctgc gcaatttcca gatggtaggc ttcttcgatg
     6721 tgggcacggc ctgggagggc gccaatccgt tcagtacaga taatccgctc aattccagcg
     6781 tcatcaccaa cggcaaccgc gtgattgtga agatcaacta cttccgcgaa ccggtggtgg
     6841 cgggctacgg cgtgggcatc cgcagtatgc ttttcggcta ctttgtgcgc gccgactacg
     6901 cctggggcat cgaaacccgc acggtgcaac ggccgaagtt gtttttggcg ctggggttag
     6961 atttttaggt cactctcccg cctcccacac ctgcaccttc ccgttcatct cattggccaa
     7021 caggcggccg tcgggcgtga agagcaggtt gtacaccggc tgccggccca gcaacagtag
     7081 cgtacgttgg ccggtgcgag cgtcccatag acaggtactc agatcggcgg atgcggtggc
     7141 tacccggctg ccgtcgggcg accaggccac agaccaaatc cgctgggcgt ggccttgaag
     7201 ggtttgtgcc aacttgcccg agggaaattc aaaaatgtgc gccgttttgt cttccgcgcc
     7261 ggagagcagc cgtttgccgt cgggcgaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ctgttctgcc tgtgtcatat
     7381 cccaaaccaa aattttcccg tcgtttccgg cggttgcaag tcggcgactg tcgggggaaa
     7441 actgtacaaa ataagtgcct gattcacagt tgattttttg tatcaaacgc ccggtctcag
     7501 cctcccagac gaatacgaaa ttgcgccggg acgccgctgc gatccatcgc ccgtcggcac
     7561 tccaggcaca accgctgctg ccgccttcaa atccctccaa agtgcgaacg gcggctccgg
     7621 tacgggcatc caaaatcggc acttttgcac cccagttgac cgcgaccaat tggctgccat
     7681 cggggttcag ggcaacggca ttggcgcctt cttccagaca gggaaactga tttttcatcg
     7741 tgccggcctg cgcattccac acggaaacct gaccgttcgg agcgcccatg gccagcgttg
     7801 tcccgtcggg gctgccggca aaagcataca catacgtatc gctgcttctc ggcgcgtcga
     7861 aagcggcatc tgcgctggcc gtccagcgcc ggatggtttt gtcgtcggaa agggtgagca
     7921 aggctttgcc gtcagatgtg ggccgtatgc gagtcacgcg acctgcatga ccgtgcaatt
     7981 gtgtgttcag gttgccggtt tcggcattcc agacatgaag tacgccttcg tctccacccg
     8041 tataaattcg gcgtccgtcg gggctgaaag caatggccat cacagcctgc cgatgccccc
     8101 gcagttcggc gatgcgcttt ccggtttggg tgttccagat gcggggttcg ccacatttgg
     8161 aggctgccgc cagaatggaa tcgttggtcg gatggaaggc gatgtcgtca atggcagtgt
     8221 aatcgttgcg gtctgaaaaa tccagtatgg ccggagcttt ggtaggttgt tgcaaatccc
     8281 agacgcccac ctgcccgtcc cagttgccgg cggctaaata cctgccgctg ctgctgaacg
     8341 cgaggcccag tgtgggtttg acgccgaatt cagcgctcca tttttctttg ccggaatgag
     8401 catcgaacag ttttatcaag ccgacgacgc cgcgcgcttt gtctctgaac cagcttgccg
     8461 ttgctacggt gctgccattg ggagagcaga ccgtcaccgc gaatccatgc ccacccgaag
     8521 aggccgtcca caactgccgg ccgctcccgg tatcccaggc gcggatgctg gaatcgcggg
     8581 acaccgaaac aatgctgctg ccgtcggggc tgaacgcgac tgaatagact gctttggagt
     8641 gcccctccaa tcgaagccgg gtttccatgc tctgcgcgtc gcggatttcc accgagccgc
     8701 cgggcatggc cagcgccaca gaacgaccat cggggctgag actgaagttg gcgggttgtt
     8761 cgccggaaat ctcataagtg ccggtgctct ggttgctgta tttgttcaaa aaatgccatt
     8821 cccagccccg gtgtttgaca ggcgctgttt ccaaccattg ccgggcttcc ggagagcgat
     8881 tgagcaaaat ggccgagttg gcggcagcta tatgagccag ataagactgg tattcctgcg
     8941 cggctttttt ttgagcgaca agcggcgttg ccaaaagcag gaataagatg aattggacgg
     9001 agatattccg cgcaggatga aacagaaatt gcatgattgg gcaaatttgg aagtggtgga
     9061 ttttgggttc aaccggcttg nnnnnnnnnn nnnnnnnnnn nnnnnnncaa aaacatcagg
     9121 tgtcaggtcc ggggattgct ccaagcgcac ccgatgtgta cacatggcgt tgaaactgct
     9181 ggtgttcttt tccaaattgg cgagcatggt ttctaaagcc ttatccatag tagattgatt
     9241 tttgtgcaaa tttgcacatt cggtgccgct attgcagttc cattttataa attgcctcca
     9301 caaaatgcgc tcatgtattc atcgccggcc atcactatgc tcacttttgc gctcggcgca
     9361 gccgctttgc acggcttttg gttgagtgcg ctgtgtgttg ggcgtgccca tacccgatgg
     9421 ctgagcctgg cattgctggg gctttccctg tacttgctca attatttgtt gttcctcacc
     9481 ggtatcattc gggcattgcc caatatgttg ggcgtgcttt tcccttttgt ttacctcacc
     9541 gggccggcgc tttatttatt catatccgat gcgctcaaca ccgacttcag attccagaaa
     9601 aatcattggc tgcacctgct gccgttcgtt tggggctggt ggcagatgcg caatgtcgtg
     9661 ggaatgccgg cgcaggaaaa aatggctatc attggctggc tgctcgatcc acaggcactg
     9721 ctcccgctaa ggtcggtact gttgggcaat ttgcacattt atgcactgtt gggatatggt
     9781 tttgcggcct ggctactggc acatcgcgcc caaaaatcag ccgaaaacga gtcgcatcaa
     9841 aaaattgccc gatggtaccg tctgttcgct ctactgttca tcgccgtttt gctgttggat
     9901 gtggcagcta aaataacttt cagcagcttg agcattccgg gaagcaacgc cgaatatgca
     9961 ctggctcttt tgccggccct cgctttgcac gccgtggggt attacgctct gcgggcaaca
    10021 ggcgggtttc cgaaaataca acccgaagca gcgcctgctg ccaaatacaa aacatcgccg
    10081 ctggatgcaa gccggttggc ggttggcaaa caagcccttt tgcaatggat ggaaacagag
    10141 cgcccctact tgaattctaa tctaaaaatc accgacctgg cccgacagat gaacatgccc
    10201 tcacaccacc tttctcagat tttgaatgaa gctattcaat tgaatttcaa tgattttatt
    10261 aatgcgtacc gcatcagcga ggcccaacgc cgactcaagg acgagcgtta ccggcacctc
    10321 tcgattgaag cgctggggat ggactgtgga tttgccaata aaacgacctt caaccgggct
    10381 tttaaaaaaa ttgcgggcac aacaccgggg gagtttttga aaaaaaacac tgaataagcc
    10441 attcaacaaa taggaaagga ggcaacttcc tacacggaaa taacctccct cccccagatt
    10501 gttcgatgat tctttttgat caatgatctt cgccccggca aatccccacg atggtgtgga
    10561 acgtatcgtg cagagatacc aattgcttgc ctacgacttc atcggcagcg ccttttttga
    10621 ccactttgtc caatttttta ctgccgctct tgagggcttt cacggcagaa atcatcgccg
    10681 gcttgttgaa ttcggcagga atggcagatt tggccaaagc cgctgccttt tcggccatct
    10741 cgccggagcg cttccgaatg ggatcaaaat tgccttcttc catcgggtgg aaagtctgcg
    10801 acatcacgct gtggaaggtt ttcagttcgg gccatttgtc catagccgat tggccggatg
    10861 cgctattgct gaacgcaatg gagaggagga atgccagtgc aattcctgaa atgattgtgt
    10921 ttttcatggt tcacttgatt ttgcggcaaa gctatcagta gcaactttca acaggtatga
    10981 cttttgtcat tggagggtat agaatcggca gttacattgg cggccaaatt ggggcatttg
    11041 tttgtttttt gggttgccgg aataccatct ttatccctaa attgcagcat tttacacaac
    11101 tattataaag ccacgaacca tgaaaagaaa gtctctactg tttttgtgca gttttctatc
    11161 tgcatttgcc ctcggggcac aaccgctgaa ccatgtttac taccgaacca ttgacggtac
    11221 ggccaacaac cagcagaatc ccacctgggg agccgccggc gctcagttgc tccgcttttc
    11281 tggcactgct tatgccgatg gtatttctgc tcccaccggc gtcaaccgcc ccaatccccg
    11341 cgccatcagc aacatgatct ttgcgcaggg caattttatg ccggctccgc aaggactgag
    11401 cgactacatg tgggtgtggg ggcagtttat agaccacgat ttcggcctca cacccgacat
    11461 caacgaaacg ttgcacattc ctataccttc cggagatgtg cattttgatc cgttcggcac
    11521 cgggcaagtc tccattcccg tgcatcgcaa cctttttgat gtgacaacgg gcaccggtat
    11581 agccaatccg cgacagcaca tcaacatcat ttcttcgtac atagatgctt ccttcgtata
    11641 tggcgaccgc aatgcacgcg cggcctggtt gcgcactttt acagggggca agctcaaagt
    11701 gtcggaaggt aatttgctgc cttacaatac tttaaatggt gaattctggg gagaaataga
    11761 tgccaatgcc ccggctatgg ccaatccggt tggactttcc aataagtttt ttgtggccgg
    11821 cgatgagcgc gccaacgaaa acccgctact ggcaggtctt catacgctct ttgtgcgcga
    11881 acacaaccgg atgtgcgatg aattggccaa acagcacccc gattggagcg acgagcgttt
    11941 gtaccagcac gcccgcaagt atgtcggcgg ctttgtgcag gccgtcactt acagggaatg
    12001 gctgccgcaa ttgggggtga acgtgccggc atacaccggt tataaaccca atgtaaatgc
    12061 cactttggcc aatgaattta ccgctgcggc tttccggctg ggacatacgc tcctcaacgg
    12121 caacctgcaa cggctttaca ataacgggga gtcgctcgaa gaaggcccgg ttgctctgcg
    12181 cgatgctttt ttcaacccct tcgccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccatgcaaca atttgacgcc aaagtcatag
    12301 acgatgtgcg caatttcctt tttggccctc cgggcgccgg cggtctcgac ctcgttgcca
    12361 tcaatattct gcgcggccgg gagcgggggc tgcccgattt taacaccatt cgggcggctt
    12421 acgggctcac gccttacagc tcgtttcctc aaattaattc ctcttctcag gtgttttctg
    12481 ccctgcacga actatacggc gatatcaaca acatagaccc ctgggtaggg tttgtggccg
    12541 agcaacagaa cagccctcaa accgtagtag gccctacttt gcagcgcatc cttcaggtac
    12601 aatttacagc attgcgcgac ggcgaccgtt tttattatga aaacgacccg gtgctgactg
    12661 acgaagagaa agacatcatc cgcaatacca aattgcgcga tatcgtggtg cgcaactccg
    12721 gcctgcccat gctgcaagac aacgtgttta aagccatgcc gcacagcgag atttgcgaca
    12781 acatgcacgt caggatttat gggcgctttc gctccgtttt ggnnnnnnnn nnnnnnnncg
    12841 ttcagcttgc agtacaaatg accggcaacg acctcgacgg ccaatccgac gcttttgggg
    12901 catatgattt caatgatgtc aaaggttgtg atgtgcaggg atttaccgtt tccaaaaacg
    12961 accctgcgtc cacgggggtt tctacgctgg atatcattct tatacagaga catatcctcg
    13021 gctctgaact tttggactcg ccatacaaac tcctggctgc cgacgtcaac agatcgggca
    13081 gtatctctac attagacatc gtgctgatgc ggcgcgtgat tctgggcatt gccacagaat
    13141 ttcccgacgg caacgtttgg cgatttattc ccaccgctca ctccttctcc gatccggcca
    13201 acccctttgc cgacaacatc atagaaccgc cgatgcattt cgatctcatg gcgctgaatt
    13261 acaacggcga tttcgttgcc gtgaaggccg gcgatgtgaa tggcagtgca gtatccggct
    13321 tccatgaaga tgaggtagaa ttgcggcagt tggcggcact tcggactttg gaccaatctt
    13381 tccgtcgggg cgaacagctt gccatacctg ttttttgcaa tgaaatcagc gggctgtcgg
    13441 gtattcagat cgaatggaac tatggctcgg aattggagct gaccgacatt gaagtacatg
    13501 gcaacagcgg gctgagtcgc gaacatttcg gcaggtttgc cacagagggc gtgctcacca
    13561 tgagttggaa ccagtcggac ttatccgaag ctttggcaaa cggagcttgg tttaccctgc
    13621 attttactgc attgaaggac gggcaactga gcgaaacgct gagacaaagc gacactaaaa
    13681 caccgtccga ggcttatacc aaaggcttgg aaacccgcca tttgctctgg tatttcgaca
    13741 cagacagcaa cgccgccgct gccggatttg aactgtatca aaacaggccc aacccggttg
    13801 cggacgccac gctcattcct ttcctgatgc ctgaatcggg gcaggtgcgc ctcagcatcg
    13861 tagatgtagc cgggcgcgtg ctgtttacag ccgaccgcca tgccgaaaaa ggatacaacg
    13921 agtggcagct tgaacgcgct caactttctg caagcggctt agtattgtat cggatgcaaa
    13981 ctccgcatgg cacgctgacg cgcaaaatgt tgctggcgga ttaaaagagt tattattgaa
    14041 tgcagaaaag gaggctgtcc gcgcaggaag cctccttttc taatttatca cccgatgaaa
    14101 ttcatcatcc ggttatttac accgtttctg ccactttgcc atacttgagc gaagcctgag
    14161 cggccgccaa tctggcaatg ggtacgcggt aaggcgagca gctcacatag tcgaggccgg
    14221 ttttgtagaa aaactctacc gatgccgggt cgccgccgtg ttcgccgcaa acgcccacat
    14281 gcaagttggg attgatctcg cgacctttct tcacggctgc atccaccaac aaacccacgc
    14341 ctttttggtc aatggatttg aacggatcgg attccaacag gccgcgcttg acgtacagcg
    14401 gcaggaattt gccggcgtcg tcgcgcgaga agcccatcgt gagttgcgtc aggtcgttgg
    14461 tgccgaagga gaagaactga gcgcctttgg caatgctgtc ggctaccata gcggcgcgcg
    14521 gcgtttccac catagtgccc accaagtagt caatgcgatc cttgcgttca gcgaagacct
    14581 gttcggctac tttgcgaatt ctggcttcct gttctttgaa ctcacgcagc gaaatgacca
    14641 agggtatcat gatttcgggg cgcaccacaa aacctttggc tttcacattg agggccgcct
    14701 cgatgatggc gcgggcctgc atttcagtga tttccgggta ggtaatgccc aagcggcagc
    14761 cgcggtgtcc caacatggga ttgaactcgt gcagttcttc cacacggtgt ttgatagctt
    14821 cgagcgagat gcccatgtcg gcggccattt gacgctgatt ttcctcttcg tgcggtacaa
    14881 attcgtgcag cggcgggtcg agcaggcgaa tggtgacggg caggtcgtgc atcgcttcaa
    14941 acaatccttc gaagtcgccg cgctgaatgg gcagcagttt ttctaaggat ttgc
//
LOCUS       DAORLF010000049        14907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2153_length_14907_cov_1.970038, whole genome shotgun sequence.
ACCESSION   DAORLF010000049 DAORLF010000000
VERSION     DAORLF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_14907_cov_1.970038"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1924
                     /locus_tag="PK198_06350"
     CDS             <1..>1924
                     /locus_tag="PK198_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38387.1"
                     /translation="TKNTFRYWFADALVSFNPGIALGTTGLSLYGFGGGASYKVARER
                     FANIVLPDSTGMSVPANMDNQIGRSLSGTIYVPDSTAGLGIKAMVALGTARREVFNGS
                     LTFEIIFNSSWGVRSIGFIGDGRFLTPPPTPGQPGPEPSLRCILDMSYDFEHEAFHAT
                     LDLYVNHMNVVKGAYPQNLAGRGVIHVDPNDWYIHLGTPQRRVMFSYSLAGLSNLARP
                     NPGNTPPPDSSGLQIDWANAGLLLTVYLDAGTILPPFPDIPQRVQSILGGGNFSIVSR
                     DDPRFASGGGVMFGASVELSIPDLSFLMFYASFGAGAGFDVMLRDYGLDARCAGNETS
                     SSPIGINGWYATGQLWGYIEGSVGIKVNLFTVKGRFEILSLAVAAVLQTRLPNPLWAR
                     GIVGGRFRILSGLVSGNCRFEFEFGKKCDIVGGSELAGIEVIASTKPDGNTAADVDVF
                     ARPQATFNLAMGEVFELQDDDGNYWQYRAKIHEFGLLKMTEGDEDGEAVAGEIRWNAA
                     SDVVGFKPDDVLDGNSPYRLVVRVRFDKKGRYEQEWRELKKLSGKSEEQEAIVDFTTG
                     PAPDYIPASNIAFSYPVRDQFNYLKQESGQGYIQLKQGQAYLFKGHPNWSYASEAGLW
                     EQKLRIMNGNQQVHIAN"
     assembly_gap    1925..2061
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2062..2873
                     /locus_tag="PK198_06355"
     CDS             <2062..2873
                     /locus_tag="PK198_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38388.1"
                     /translation="LVNLQTSPTDTAFTNIMLEERVAATVQRGRQPRNFYASHFRTSL
                     YNTFIQKMNALNMSQSWLNPVFLDPNVTSGASINDFGVFVTGNELFDLFDQTGYDNGD
                     TTLAPLVQPIADLSVTGGNWYNNSVYPYMYQHFPAAAQDITLSWRNPAILGDVPAKAV
                     SIYQLDTPHRLTEEDVQTRTAIIPQPVTGLAYQVPFNALFDSFDYKHKVLNFMASNPN
                     TALPAAWINFVQGNMQYPPFGTYRVKLEYRLPGINTLTSQHTLNLIYGTSN"
     gene            2857..5121
                     /locus_tag="PK198_06360"
     CDS             2857..5121
                     /locus_tag="PK198_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38389.1"
                     /translation="MEPVIDRRAAQLAVLAFCLLPFAFCQAQQGINPPASEAETSDAL
                     MITGRSTGGSILLRWAPGSPAVWRRCNEVGYMVVRHTMMRNNQLLSWEERSVAVPLSG
                     EPIMPWKTEAQWVPLMARDSFAAIAAQALLGEDFELQVPKESVGGLVNRATEENNRFA
                     FGMFAADHSYQAARALGLAWEDKEVKPNETYLYRVFPARAFTVGADTVRIGDQGQPIP
                     LEYRTAIDTGFFSISPKDKFPLPKILEVEADFGNKMATVSWNKNRFAQFYVSYRVERS
                     SDGQIWESLHDRPLITLDRPGAPTDFMFMTDSLPMNNKPYFYRVSGRTVFDDFGPPSD
                     PVQGMGIDPLPEFYPYVVSVLESEQGGFIVGWEFNPGDEDKILGFKLFRSNTDLGRYE
                     PVSGDLLLPPFQRAFTDESPLPVNYYRVSAFDVYNREMSSFSALAQLNDETPPAAPKN
                     VRGAILKDGATVITWDANTEPDLAGYRVYVANHPNDEFSQITSVPVKSNHFIDTVSLN
                     TLNKELYVQVIALDYRQNSSDFSKTGILLRPDTIAPAPPAFSNLSADAAGVHLEWANS
                     QSLDVLRHELLRRPQGAAQWETVTALPFVPGERRSSYSDTTGTIGKDYEYQIVAVDNS
                     DLRGASQTVEARRLDNFIRPAVSALTTEADRRNKWVLLAWKHRDLSAIRLFEVYRAEG
                     EGPLNRYRTYTPQNIVEQPAAGTNKKQASAKQHSFSVQDSAVQMNKSYRYAVRAIYND
                     GGMSPLSEAVLIKY"
     gene            5187..>7568
                     /locus_tag="PK198_06365"
     CDS             5187..>7568
                     /locus_tag="PK198_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38390.1"
                     /translation="MSAKITIAFVLLLFAAAGTLPAQNLKATLKMTRGWCKQMGGDQS
                     WPPDPTWKTRASIKTSSSDTGVNGEICRYLDNYRPLLNSPDCNSGLEMFSSFIFPGNC
                     APREKVYDDLILTYQPSSYNFSLADGFYVTINMEAWEDDKGGICDINSGDDDVSKKDS
                     TFWMRIPNGATFPYHYDFTLRTENQAFRVSYRLTVENLDAISNTSMRVANSAGTTQNS
                     FCSGDPVRLYADRRAGFSGGYFEWQRRATPSSSWVSATSTTYTTSNFLEVSAANPMPD
                     YRVRVAQSGANGTATQDGWIEVSGSTITVAPAPPALSNIATTITGACSGSSNGAIQVV
                     VSNSNASSQYLLTLVNSSNQTVSSHTPALGSTLTHTFTGLSAGTYKVKVGFSGSSVDC
                     TTQTGNITVNTLQLPVISASGAAGNCAGENGSVTVNITSNASGTNTYTLLNATGNTVL
                     QTQNTTQASYTFSVAAGTYRVRVTNGNGCQSALSGQVTVPNAPSAVAASAAPVSVGGI
                     YHIRCFGGEGAVQIIPSGGTPPYRVGVAGLPLIFNIQSGQTVSFSRPAGTYAYTVTDS
                     KGCTANGSVTLVQPPQNLSVAISNIQQASDCAPTGSATFGGSGGSGTYQYSLDNPSGP
                     YTTNPVFNNLAAGAHIAYIRDLTGCQANASFNVGAPNPLLLEVTAVSNVLCAGSNEGS
                     VTLSASGGNPPYQFRIGNGAFSAQNTFNGLTADTYTFEVRDADNCANTTMQNVAAPNA
                     IIITGHEISDLDCEGLTRNVVITFTGRADGVPNFLDNGLEISTDNGQSYNGFYE"
     assembly_gap    7569..7688
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7689..>8950
                     /locus_tag="PK198_06370"
     CDS             <7689..>8950
                     /locus_tag="PK198_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF38391.1"
                     /translation="TYTVNVAGGQNLFINLNSTTDETCAGANDGTITVSYGGGVGTYY
                     LILLRQYSSDEFEEMQTLGPVSQPCDYTFTNVPVFLPGGFNQYLVRVVDGFSGIPSNY
                     NFRCAGSVGNIFIYPNTPFNSGTAQITGPLLNCYGNNGQITITGTSGGTAPYEFSQDG
                     EEFQSSNVFSGLGAQNSFYVRDSRGCKQGPINITIPSAPVTLNATATLTNSETACTKG
                     SIQVNITGGTGPFEVALIQDGDCSAAYLFAAVTSSTSIPFTELSAGNYTVCIRDAGGC
                     RADRSVAVPFVAEPNMVVTATQSTTCFGGGNNGAITLQASGGTPPYTITINNGSPQTG
                     SNSFNFSSLAPGSYLFQLTDAKGCTDLLVGNVPSNNLLSASVDADAISGCAGGNTGVI
                     RAIPYDGVAPYTVTWLWDNVTVSGVAEN"
     assembly_gap    8951..8965
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8966..>10414
                     /locus_tag="PK198_06375"
     CDS             <8966..>10414
                     /locus_tag="PK198_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF38392.1"
                     /translation="RTGMPAGVYQVRITDAIGCQRIETEIVETPLPLSANLLVIDALC
                     SNVLNGSVTINAVGGWEVYEYALGAGPFQTDNSFENLAPGNYTVTVRDTSNCTVSYPF
                     TIGLQRVITANATPANPACFGQMNGSIAIAAVGGGAPYTYSANNGTFLPGNPITGLGQ
                     GTYTVVLRDNGGCEFTINNIQLTEPPQLTVTANLLQDASCANNAGNLSASPNGGTPGY
                     SYQWDGNPALNLATYNNAPPGMHTVVVTDSRGCTASASATVSNVPGVALNLVGTTNAL
                     CNLANGTATVAVQGGIPPFNYTWSHNAGLNSPMATGLAAGPYSVTVTDGNNCSAVRSL
                     TVAASSTVSLNITEVKNSFCQEGNGRITVAPVGGTPPFNYTWSHNAGLNSPTAAGLFS
                     GSYSVTVTDGNNCQSSTSANISLETGPTATLSVQNTACQGNTGSVQVLVSGGTGPYTY
                     TWSHDAGLHSNIASNLFAGVYFCTVTDANGCIF"
     assembly_gap    10415..10430
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10431..>11411
                     /locus_tag="PK198_06380"
     CDS             <10431..>11411
                     /locus_tag="PK198_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38393.1"
                     /translation="STTVTTLPPPTAFVSATTATCSLANGSAQALVSGGTAPYTYNWS
                     SGSPNSPTAQNLAGGDYVLLVTDAFGCQTIAQFTVGNIPGPTGLSVNFQNSLCTNFNG
                     SISVSPQGGTGPFNYVWSHNAFLNQATANLLPAGTYSVTATDANGCQVSATQTIVLQG
                     PPTIQTVQQINSLCQNGTGLLEVAASGTGLFQYTWTGGVSTGPVAQNLNAGNYTVTVS
                     DANNCQTTRSFNIALEPAPAILLVSQANDFCGRGLGSIRIRSVGGSAPITFSWSHNPA
                     LSSDWLTGLTAGTYSVTATDANGCQATAQYVITETPGPELVVIGTTTAFCG"
     assembly_gap    11412..11546
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11547..>14907
                     /locus_tag="PK198_06385"
     CDS             <11547..>14907
                     /locus_tag="PK198_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38394.1"
                     /translation="DANGCTATATATVESTTPPVLSLAATGDNPCVIQDAAITFSIAG
                     GAAPYAYAWSHNPGLNSLSANGLATGTYTITATDANGCQATLSATVTDRRGPVLQLLN
                     TTPSTCGFSDGSAAVGASLGQAPYQYAWSHNAALSGNTAANLAAGNYSVTVTDANGCT
                     DVVSFNISDSQAPQAQVSHAADAVCMPNSGSMTVSASGGVGPYQYAWSHQPGLNSATA
                     GGLAAGSYVITVTDSNGCQAVATGSIGFQAPPSATAAATDALCEPNTGAISVSVSGGQ
                     APYNIAWNIPALSGFQPAPVFAGSYSATITDANGCVFSLPVEVNFIPGPTVVLVQQQD
                     ASCGQSDGSVQVGEIGGAFPYFYSWSHDPQLNFFIANNLPSGNYTVSITDANGCSGTL
                     STTLADVSAAVLTFSADNSDCGQSNGSATVSAVGGALPLSYAWSHNAGLNSPAAANLP
                     AGLYSVTVTDANGCIAEVSGEVEDEGGVSLSIAGFANAVCEANSGSITLTATGGTGPY
                     QYIWSHNAGLNQANATGLTAGTYRVTATDANGCQDVVSQTLTFTAGPQLTLANATNSL
                     CQDGNGALSFTAAGGTGALSYQWSHQGGLNAGSATGLSAGSYTLTVTDANGCTATQTA
                     QISLIAGPQLSIASQTDAYCGNDAGALSIAVTGGTGPFQYLWSHDAQLPSASANNLSA
                     GTYSLTATDANGCVAEISATVADVPGFTLENPTVQATTCGNDNGQIQVTLLGGQAPFQ
                     YQWSHSATLNAPTATGLPVGVYGVTVSDATGCTRDTAIAVQSADGPVLSISQIQNATC
                     GLANGSMATTISGGQAPFDYAWSNGSTQPSISGLAGGAYQLTVTDANGCQGVAFAELT
                     AGTAPVLQLLNTIETGCTNAVGALQFVATGGQAPYSYAWSHNAGLNSASASGLAAGNY
                     TLSLTDAAGCTATAAADVQAQSDLGLNLVQTQNAICTSATGSAQVSSTGGQGPYSFAW
                     SHDAALTTGLASNLVAGSYSVTATDAEGCTAQLSLTVGTQNEPPQLALVQTTDSDCNG
                     STGSIEVSATGAQGPYTFVWSHDAGLNSNIAGNLSAAGYTVTATDANGCTRTEVAQGG
                     IFAEPDLGTTLAHVSCANAQDGSIAVGLFGGT"
BASE COUNT         3165 a         4570 c         3895 g         2854 t
ORIGIN      
        1 caccaaaaac accttccgat attggtttgc cgatgcactg gtgagtttca accccggtat
       61 tgcgctgggt accaccggcc tgagtttgta cgggttcgga ggcggcgcat cgtacaaggt
      121 ggccagggaa cggtttgcca acatcgtttt gcccgacagc accggcatgt ctgtgcccgc
      181 caatatggac aaccagatcg ggcgctccct ttcgggaacc atttatgtac ccgacagcac
      241 cgccggactc ggcatcaaag cgatggtggc tttgggaact gcccgccgcg aggtattcaa
      301 cggcagcctc acttttgaga tcattttcaa cagcagttgg ggcgttcgca gcatcggctt
      361 catcggcgac gggcgattcc tgaccccgcc gcctacaccc ggacagcccg gcccggaacc
      421 cagtctgcgc tgcatcctcg atatgtctta tgattttgaa cacgaggctt tccatgccac
      481 gctcgacttg tacgtcaacc acatgaacgt ggtgaagggc gcatacccgc aaaacttagc
      541 cggccggggc gtcatccatg tggaccccaa cgactggtac atccacctcg gcacgccgca
      601 gcgccgcgtc atgttcagct actcactggc gggcctgtcc aacctggctc gccccaatcc
      661 gggcaacacg ccgccacccg actcctccgg cctgcaaatt gactgggcca acgccgggct
      721 gttgctcacc gtatatttag atgccggcac catcctgcca ccctttcccg acataccgca
      781 gcgcgtacaa agcattttgg gcggcggcaa tttcagcatc gtgagccgcg acgacccgcg
      841 ctttgcctcc ggcggcggcg tcatgttcgg cgcttcggta gaattgagta ttcccgacct
      901 gagcttcctc atgttttatg cctcctttgg cgcaggcgca ggtttcgatg tgatgctgcg
      961 cgattacggc ctcgacgccc ggtgtgccgg caatgaaacc agcagcagcc ccatcggcat
     1021 caacggctgg tacgccaccg ggcagttgtg gggctatatt gagggttccg tcggcatcaa
     1081 agtcaatttg tttaccgtga agggccgttt tgaaattctc agcttggccg tagccgccgt
     1141 attgcaaacc cgcctgccca acccgctctg ggccagaggc attgtgggcg gcagattccg
     1201 catcctcagc ggattggtga gcggcaattg ccggttcgag tttgaatttg gtaaaaaatg
     1261 cgacatcgtg ggcggcagcg aactggcagg tattgaggtc attgcaagca caaaacccga
     1321 cggcaacacc gccgccgatg tggacgtatt tgcgcgcccg caggctactt tcaacttggc
     1381 gatgggcgag gtgttcgagc ttcaggacga cgacggcaac tactggcagt accgcgccaa
     1441 gatacatgag ttcggcctgc tgaaaatgac cgaaggcgat gaagacggag aagccgttgc
     1501 cggcgaaata cgctggaatg cggcgtccga tgtggtgggt ttcaaacctg atgacgtatt
     1561 ggacggcaac agcccttacc gattggtggt gcgggtgcgg tttgataaaa aaggccgtta
     1621 tgaacaggaa tggcgggagt tgaaaaaact cagcggcaag tcggaagaac aggaagccat
     1681 tgtggacttc acaaccggcc ccgcgcccga ttacattccc gcctccaaca tagccttcag
     1741 ctacccggtg cgcgatcagt tcaactacct caagcaggaa agcgggcagg gctacatcca
     1801 gctcaagcag ggccaggctt atttgttcaa aggccatccc aactggagct acgcctccga
     1861 ggccggcctg tgggagcaaa aactgcgcat tatgaacggc aaccaacagg tacacatcgc
     1921 caatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn naattggtca atctgcaaac cagcccgaca gacacggcct
     2101 tcaccaacat tatgctcgaa gagcgcgtag ccgccaccgt gcagcgcggc cggcagccca
     2161 ggaatttcta cgcgtcccac ttccgcacca gcttgtacaa taccttcata caaaaaatga
     2221 atgcgctgaa catgagtcag tcatggctca acccggtctt cctcgatccg aatgtgacat
     2281 cgggagccag catcaacgat ttcggcgtgt tcgttaccgg caatgaattg ttcgaccttt
     2341 ttgaccaaac cggctacgac aacggcgata ccacgctggc gcctttggtg caacccatcg
     2401 ccgacctgag cgtgacgggc ggcaattggt acaacaactc ggtgtatcca tacatgtacc
     2461 agcatttccc ggcggcggcg caagacatta cgctgagttg gcgcaatccg gccatactcg
     2521 gcgacgtgcc tgccaaagcg gtgagcatct accagttgga tacgccgcac cgcctcaccg
     2581 aggaggacgt gcaaacccgc acggcgatca ttccgcagcc cgtcaccgga ttagcctatc
     2641 aggtgccgtt caacgccctg ttcgacagct ttgattacaa acataaagtg ctgaatttca
     2701 tggcatccaa tccgaatacc gccttgccgg cagcctggat caactttgta caaggcaata
     2761 tgcagtatcc gcctttcggt acttatcgcg tgaagctgga gtatcgcttg ccgggtatca
     2821 acacacttac ttcacaacac accctgaatt tgatatatgg aaccagtaat tgatcggaga
     2881 gctgcgcagt tggcggtgct tgccttttgc cttttgcctt ttgctttttg tcaggcgcaa
     2941 caaggcatca acccgcctgc atctgaagcg gagacatccg atgcactgat gattaccggg
     3001 cgcagtacgg gcggcagtat cctgttgcgc tgggcgccgg gatcgccggc agtatggcgg
     3061 cgttgcaacg aggtgggcta catggtagtg cggcacacca tgatgcgcaa caaccagctc
     3121 ctgtcctggg aagagcgctc ggtggccgta ccgctgagcg gcgaacccat catgccctgg
     3181 aaaacggagg ctcaatgggt gccgctgatg gcacgcgact cctttgccgc cattgccgcg
     3241 caggcattgc tgggcgagga ctttgagttg caggtgccca aagagtcggt cggaggtttg
     3301 gtcaaccggg ccaccgaaga gaacaaccgc ttcgcctttg gcatgtttgc cgccgaccat
     3361 tcctatcagg cggcgcgggc attgggtttg gcctgggaag acaaagaggt gaagcccaat
     3421 gaaacctacc tgtaccgggt gtttcccgca agggctttca ccgtcggtgc ggatacggtg
     3481 cggataggcg atcagggcca acccattcct ttggagtatc gcacggccat agacaccggc
     3541 tttttcagca tcagcccgaa agacaagttt ccattgccga aaatattgga agtggaggcc
     3601 gattttggaa acaaaatggc tacggtgagt tggaataaaa accgctttgc gcaattctat
     3661 gtcagctacc gcgtagagcg ctcttccgac ggccagattt gggaatcgct ccatgaccga
     3721 ccgctcatca cgctcgaccg gcccggcgcg cccaccgact tcatgtttat gaccgacagc
     3781 ctgcccatga acaacaagcc ctacttctac cgggtgagcg ggcgcacggt gtttgacgat
     3841 ttcgggccgc cttccgatcc ggtgcagggc atgggcatag accccctgcc ggaattttat
     3901 ccctacgtcg tttctgtgtt ggaaagcgag cagggcggct tcattgtggg atgggagttc
     3961 aatcccggcg atgaagataa aatattggga ttcaaactat tcagaagcaa taccgacctc
     4021 ggcaggtacg aacccgtcag cggcgacctg ttgctgccgc ctttccagcg cgccttcacc
     4081 gatgaatcgc cgttgcccgt caattactac cgcgtctcgg ctttcgatgt ttacaaccgc
     4141 gagatgtctt cgttttcggc attggcccaa ttgaacgacg aaacgccgcc cgccgccccc
     4201 aaaaacgtgc gcggcgccat cctcaaagac ggcgccaccg tcattacctg ggatgccaat
     4261 accgaacccg atctggcggg ctaccgcgta tatgtggcca accaccccaa cgacgagttt
     4321 tcacaaatca cctccgttcc cgtcaagagc aaccacttca ttgacacggt aagcctcaat
     4381 acgctcaaca aagagctgta tgtgcaggtg atcgcgttgg attaccgcca gaactcctcc
     4441 gatttttcaa aaaccggcat cctgctccgc cccgacacca ttgcgcccgc gccgcccgct
     4501 ttcagcaatc tgagtgccga tgccgccggc gtacacctcg aatgggccaa cagccaaagc
     4561 ctcgacgtac tccgccacga actcctccga cggccccaag gcgccgctca gtgggaaacc
     4621 gtcaccgcgc tgccttttgt acccggagaa cgccgcagca gctattccga caccaccgga
     4681 accatcggaa aagactatga atatcagatc gttgcagtgg acaattccga cctgcgcggc
     4741 gcctcgcaaa ccgtggaagc ccgccgattg gacaacttca ttcgccctgc cgtttcggcc
     4801 ctgaccaccg aagccgaccg gcgcaacaag tgggtgctgc tggcctggaa acaccgcgat
     4861 ctctcggcca ttcgcctctt tgaggtgtat cgcgcagagg gcgagggccc tctcaatcgc
     4921 taccgcacct acacgccgca aaacatcgtg gagcagcccg cagccggaac gaacaaaaag
     4981 caggcttccg ccaaacagca cagcttttcg gtgcaggatt ccgccgtgca gatgaacaaa
     5041 tcataccggt atgccgtgcg cgccatttac aacgacgggg gcatgtcgcc gctgagcgaa
     5101 gccgtactca tcaagtattg agcgccgaaa acctgccgac aattcagaaa aacaaccata
     5161 aacctttgac tcgcaatcct ttgcttatga gcgccaaaat aaccatcgca tttgttttgt
     5221 tgctgttcgc tgcggcgggc accctccctg ctcaaaacct caaagccacc ctgaaaatga
     5281 cgcggggctg gtgcaaacaa atgggcggcg accaaagctg gccacccgac ccgacctgga
     5341 aaacccgtgc ctctatcaaa accagcagct cggataccgg tgtaaacggc gaaatctgcc
     5401 ggtatttgga caactaccgt ccgctattga actcgcccga ctgcaattcg ggcctggaaa
     5461 tgttttcttc ttttatattt cctggtaatt gcgccccccg cgaaaaggta tatgacgacc
     5521 tgatattgac gtatcaacca tcctcataca atttttcatt ggccgacggt ttttatgtca
     5581 ccatcaacat ggaagcctgg gaagacgaca agggtggtat ctgcgacatc aactccggcg
     5641 acgacgatgt cagcaagaag gactctacct tttggatgcg catacccaat ggcgctactt
     5701 ttccatatca ttatgatttt acgctccgaa ctgaaaacca agcctttaga gtgtcctatc
     5761 gcctgaccgt tgaaaattta gacgccatca gcaatacctc gatgcgtgta gccaacagtg
     5821 caggaaccac ccaaaacagt ttttgcagcg gcgaccccgt gcgcctgtat gccgaccgca
     5881 gggcagggtt cagcggtggc tattttgaat ggcaacggcg tgccacccct tcctcaagtt
     5941 gggtttcggc aacctctacg acctatacta cctccaactt tttggaagta tcggccgcca
     6001 accccatgcc cgactaccgg gtgcgggtgg ctcaaagcgg cgccaacggc actgccaccc
     6061 aagatggctg gatagaggtg tccggctcta ccattaccgt agcccctgcc ccccctgcgc
     6121 tcagcaacat cgccaccact ataaccggcg cttgttcggg cagcagcaac ggcgccattc
     6181 aggtggtagt gagcaactcc aacgcctcct cacaatacct gttgacattg gtcaattcga
     6241 gcaatcagac tgtatcgagt catacgccgg cattgggcag caccctcacc cacacattta
     6301 ccggtttgag cgcaggcacc tacaaagtca aggtaggctt ttccggcagc agcgtggatt
     6361 gcaccacaca aaccggcaac atcacggtga acaccttgca gttgccggtc atttccgcat
     6421 cgggggcggc cggtaattgt gccggcgaaa acggtagcgt aaccgtcaat attacctcaa
     6481 atgcttcggg tacaaatact tacacactgc tgaatgcaac cggcaacacg gtgctgcaaa
     6541 cgcaaaacac cacccaagcc tcttacacgt tcagcgtagc ggcgggtacc taccgcgtgc
     6601 gggtgaccaa tggcaacggc tgccagagtg cgctctccgg tcaggttacg gtgcccaacg
     6661 cgccttctgc tgttgccgca tccgcagcgc ctgtgtcggt ggggggcatt tatcacatca
     6721 ggtgcttcgg aggcgaaggc gctgttcaga ttattccttc gggaggcacc ccgccctacc
     6781 gggtaggagt ggcagggctg cctttgatct tcaatattca gtctggccag acggtttcat
     6841 tttcgcgccc ggccggaacc tacgcctaca ccgtcaccga cagtaaaggc tgcaccgcca
     6901 acggctcggt tacattggtg caacctcctc aaaatctttc tgttgcgatt tccaatattc
     6961 agcaggcttc cgactgcgcc cccaccggct cggccacatt cggcggcagc ggcggttcgg
     7021 gtacctacca atatagttta gacaacccca gcggccctta caccaccaac cctgtgttca
     7081 acaatctggc agcgggcgcg cacattgcat atatccgcga tctgaccggc tgtcaggcca
     7141 acgcgtcctt taatgtcggg gcgcccaatc cattgttgct ggaggtcacg gccgtcagca
     7201 atgtactttg tgcaggcagc aacgaaggct ccgtcacgct gtcggcatcc ggcggcaacc
     7261 cgccttatca gtttcggatc ggcaacggcg cattttctgc tcaaaacacc ttcaatggcc
     7321 tcacagcaga tacttacacc ttcgaagtac gcgatgcaga caattgtgcc aatacgacca
     7381 tgcaaaatgt agctgcgccc aatgccatca tcatcaccgg ccacgaaatc agcgatctgg
     7441 actgcgaagg actgaccaga aatgtcgtca tcacctttac cggccgggcc gacggcgtgc
     7501 ccaacttctt agataacggg ctggaaatct ctaccgacaa cggccaaagc tacaacgggt
     7561 tttatgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnac acttatacgg tgaatgtggc gggcggtcag aacctgttca tcaacctcaa
     7741 cagcaccacc gacgaaacct gtgccggggc caacgacggt accatcaccg tgagctacgg
     7801 cgggggagtg ggaacgtact atctgatatt gctgaggcag tattcttcag atgaatttga
     7861 agaaatgcag acattaggac cggtttcgca gccgtgcgat tatacgttca ctaatgtgcc
     7921 ggtcttcctg ccgggcggtt ttaatcaata tttggtaaga gtggtggacg gattcagcgg
     7981 catcccgtca aactacaatt ttcggtgtgc gggaagcgtc ggcaacatat ttatctatcc
     8041 caatacgcct ttcaacagcg gtactgctca aatcaccggc ccgctgctca attgctacgg
     8101 caacaacggc cagatcacca tcaccggcac ttcgggaggc acggcgcctt atgagttttc
     8161 acaagatgga gaagagttcc agtcgagcaa tgtattctca ggtctcggcg ctcaaaacag
     8221 cttttatgtg cgcgacagcc ggggctgcaa acaaggcccc atcaacatca cgattccatc
     8281 tgcgcccgtc accctgaacg ctacggccac cctgaccaat tcggaaaccg cgtgtaccaa
     8341 aggctccatt caggtcaaca tcacgggcgg cacggggccg tttgaggtcg ctcttattca
     8401 ggatggcgat tgctcggctg cttacctttt tgctgccgta acgagcagta ccagcattcc
     8461 tttcaccgag ctttcggcag gcaactacac cgtttgtatc cgagatgcag gcggctgccg
     8521 ggcagatcgc agtgttgccg tgccttttgt ggccgaaccc aacatggtcg ttactgccac
     8581 gcaaagcact acctgtttcg gtggcggcaa caacggcgcc attaccttgc aagcctccgg
     8641 cggcacaccg ccctacacca tcaccatcaa caacggctcg ccgcaaaccg gcagcaattc
     8701 ctttaatttc agcagcttag ctcccggaag ctacctattc caactcaccg acgccaaagg
     8761 ttgtaccgac ctcttggtgg gcaatgtgcc gtccaacaac ctgctctccg ccagcgtgga
     8821 cgcagatgcc atcagcggtt gtgcgggtgg taataccggc gtcataagag ccattcctta
     8881 cgacggtgtg gctccctaca cggttacctg gctttgggac aacgtaacgg tgagcggagt
     8941 ggccgaaaat nnnnnnnnnn nnnnncgcac aggcatgccc gccggtgtgt atcaggtgcg
     9001 catcaccgat gccattggtt gccagcgcat tgagacggaa atcgtggaaa ccccgctgcc
     9061 cctctcggcc aacctcttgg taatagacgc cctgtgttcc aacgtgctca acggctccgt
     9121 gaccatcaat gcggtcggcg gctgggaagt gtatgaatat gccctcggcg ccgggccgtt
     9181 ccagacagat aattcctttg aaaacctcgc gcccggcaac tacacagtca ctgtgcgcga
     9241 tacgagcaat tgcaccgtca gctacccctt caccattggt ttgcaacgcg ttatcacagc
     9301 caatgccacg cctgcgaacc cggcttgttt cggccagatg aacggctcca ttgccatagc
     9361 ggcagtgggc ggcggcgcgc cttacaccta ctcggccaac aacggcacct tcctgcccgg
     9421 caacccgatc accggcttgg ggcagggtac ttacacggtc gtcctgcgcg acaatggcgg
     9481 ctgcgagttt accatcaaca acatacaact cactgaaccg ccgcaactta ccgtcaccgc
     9541 caacctgctg caagacgcct cctgtgccaa caacgcgggc aacctttcgg cttcgcccaa
     9601 tggcggcacg cccggctatt cctaccaatg ggacggcaac ccggcgctca acctggcgac
     9661 ctacaacaac gcgcctcccg gtatgcacac cgtcgtcgtc accgactcca gaggctgtac
     9721 ggcctcggct tccgccacgg tttccaatgt gcccggcgta gcgctcaact tggtgggtac
     9781 gaccaatgcg ctgtgcaatc tggccaacgg caccgccacc gtagccgtgc agggcggcat
     9841 ccctccgttt aactacacct ggagccacaa cgccggcctg aacagcccga tggccaccgg
     9901 tttggcggca ggcccttact ccgtcaccgt gacggacggc aacaactgct ctgccgttcg
     9961 cagccttacc gtggcggcct ccagcacggt gagtttgaac attacggagg taaaaaattc
    10021 cttctgtcag gaaggcaacg ggcgcatcac cgtagcgcct gtgggcggta ctccaccgtt
    10081 caattacact tggagccaca atgccggctt gaacagcccg accgcagccg gcctcttctc
    10141 cggcagctat tccgtgaccg taaccgacgg caacaactgc cagagttcca cctccgcgaa
    10201 catatcgctg gaaaccggcc caacggccac cttgtcggta caaaatactg cctgtcaggg
    10261 caatacaggc agcgtgcaag tgctcgtttc gggcggcacg gggccgtaca cctacacctg
    10321 gagccacgac gccggcctcc attccaacat cgcctccaac ctctttgcgg gagtctattt
    10381 ctgtaccgtg acggatgcca acggttgtat ttttnnnnnn nnnnnnnnnn ttccactacc
    10441 gtcaccacac tgccgccgcc gacagccttc gtctccgcca ccaccgccac ctgctccctc
    10501 gccaacggca gcgcgcaggc attggtgtcg ggtggcacag cgccttatac ctacaactgg
    10561 agcagcggca gccccaacag cccgaccgcc caaaacctcg cgggcggtga ttacgtcctg
    10621 ttggtaacag acgcattcgg atgccagacc atagcgcagt ttaccgtggg caacattccc
    10681 ggccccaccg ggctttcggt gaatttccaa aacagcctgt gtaccaattt caacggcagc
    10741 atctcggtgt ctccgcaggg cggtaccggg ccgttcaact atgtgtggag ccacaatgcc
    10801 ttcctcaacc aagcgacggc caatctcctc cctgccggaa cctactccgt gaccgccacc
    10861 gacgccaacg gatgccaggt atcggccaca caaaccattg ttttacaagg gcctccgacc
    10921 atccaaaccg tacaacagat caacagcctg tgccaaaacg gaaccggcct ccttgaagtg
    10981 gctgcctccg gaacagggct gttccaatat acctggacgg gcggcgtttc taccggacct
    11041 gtggcgcaaa acctcaatgc gggcaactat accgttacgg tttcggatgc caacaactgc
    11101 cagaccaccc gcagtttcaa catcgcgctg gagcctgcac cggccatttt actcgtctct
    11161 caagccaatg atttctgtgg ccggggatta ggttccatca gaatccgcag cgtgggaggc
    11221 agcgcgccca ttaccttcag ttggagccac aaccccgccc tgagcagcga ctggctcacc
    11281 ggcctcacgg caggcactta cagcgtcacc gccaccgatg ccaatggttg ccaggccaca
    11341 gcgcagtatg tcatcaccga aacgccgggg cctgaattgg tcgtaatcgg cacaacgaca
    11401 gcattttgcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnncgga cgccaacgga tgtaccgcca ccgccaccgc
    11581 taccgtagaa agtaccacgc cgcccgtact gtcattggca gctaccggcg ataacccctg
    11641 cgtgatacaa gacgcggcca tcaccttcag catcgcggga ggcgccgcgc cgtacgccta
    11701 cgcctggagt cacaaccccg gcctgaacag cctttcggca aacggtctcg caacgggtac
    11761 ttacaccatc acggccaccg acgccaacgg atgccaggcc acgctgagcg caacggtgac
    11821 ggatcgcaga gggccggtat tgcagctact caacaccacg ccgagcacct gcggattttc
    11881 cgacggcagt gcagcggtgg gagcttcttt gggccaggct ccgtaccagt acgcctggag
    11941 ccacaatgcc gccctttcgg gcaataccgc cgccaacctc gctgccggta attactctgt
    12001 gacggtaacg gacgccaacg gctgcaccga tgttgtcagc ttcaatatct cagattctca
    12061 ggcgccgcag gctcaggtga gccatgccgc cgacgccgtt tgtatgccca acagcggctc
    12121 catgaccgtg agcgcatcgg ggggcgtagg gccgtaccag tatgcgtgga gccaccagcc
    12181 gggtttgaat tcggcgacag ccggcggact cgcagcaggc agctatgtca ttacggttac
    12241 cgacagcaat ggttgtcagg cagttgctac gggcagcatt ggatttcagg cgccgcccag
    12301 cgcaacggcc gctgccaccg acgcactttg cgaaccgaat accggcgcca tatcggtgtc
    12361 cgtcagtgga ggtcaggcgc cctacaacat cgcctggaac atacctgcgc taagcgggtt
    12421 ccagcctgcg ccggtgtttg ccgggagcta ctcggccacc atcaccgatg ccaacggatg
    12481 cgtgttcagt ctgccggttg aggtcaactt tatacccggc ccgacggtgg tattggtaca
    12541 acaacaagat gcctcctgcg gccaatcgga cggttcggtg caggtgggcg agatcggagg
    12601 cgcgtttcct tacttctact cctggagcca tgatccgcaa ctcaattttt ttattgcaaa
    12661 caacttgcct tcaggcaatt acaccgtcag cataaccgat gccaacggct gctccggcac
    12721 actctccacc accttagccg atgtgtctgc cgctgtactc acgttctctg ccgacaacag
    12781 cgactgcgga cagagcaacg gcagtgccac ggtgagcgct gtgggtggcg cgctcccgct
    12841 gagctacgcc tggagccaca atgccggcct caacagcccc gctgctgcca acctgcccgc
    12901 cgggctgtat tccgtcaccg tcaccgatgc caacggctgt attgccgaag tgagcggcga
    12961 ggtggaggac gaaggcggcg tgagtctgag cattgccggt tttgccaatg ctgtttgtga
    13021 ggccaattcg ggttccatca ccctgacggc aacgggtgga acggggccat accaatacat
    13081 ctggagccac aacgcaggac tgaatcaggc caatgccaca ggattgacgg cgggtactta
    13141 ccgggtcacc gcgacagacg ccaacggttg ccaggacgtg gtttcccaaa ccctcacctt
    13201 cacagcgggg ccgcaactga ccttggccaa cgcaaccaat tcgctgtgcc aggacggcaa
    13261 cggcgcgttg agttttaccg ctgcgggcgg aaccggcgct ctgagctatc agtggtcgca
    13321 tcagggcggt ttgaacgcag gttcggccac cggtctctct gccggcagct acaccctcac
    13381 cgtcaccgac gccaatggct gcaccgccac ccaaacggcg cagatcagcc tcatcgccgg
    13441 gccgcaattg agcatagcct cccaaacaga cgcctattgt ggcaacgacg ccggcgctct
    13501 gagtatcgcc gtgacaggcg gaaccgggcc tttccaatat ctctggagtc acgatgcaca
    13561 gttaccatcg gcttccgcca ataatctcag cgccggaact tactccctga ctgccactga
    13621 tgccaatgga tgtgtggccg aaatcagcgc gaccgtagcc gatgtgccgg gattcacttt
    13681 ggaaaatccg accgtgcagg ccaccacttg cggcaacgac aatggccaga tacaggtcac
    13741 gcttctcggc gggcaggctc ctttccaata ccaatggagc cacagcgcga cgctgaacgc
    13801 ccccaccgcc acaggtctgc cggtgggtgt gtatggagta acggtaagcg acgccacggg
    13861 ctgcacccgc gacaccgcga ttgccgtgca aagtgcggac ggcccggttt tgagcatttc
    13921 tcagatacaa aatgccacct gcggcctggc caacggcagc atggccacca ccatcagcgg
    13981 cgggcaagcg ccttttgact acgcatggag caacggcagc acccagcctt cgatctccgg
    14041 tttggcgggc ggagcatacc agctcactgt caccgatgcc aacggatgtc agggggtggc
    14101 tttcgccgaa ttgacggcag gcaccgcgcc ggtactccaa ctgctgaata ccattgaaac
    14161 cggttgcacc aatgccgtag gcgcgctgca attcgtcgca acaggcggac aggcgcccta
    14221 ctcttatgca tggagccaca acgccggcct gaacagtgcc tcggcctccg ggctggcggc
    14281 gggcaactac accctcagcc tcactgatgc ggcgggttgt acggccacgg ctgctgccga
    14341 tgtacaggcg cagagcgatc tggggctgaa tttggtacaa acccaaaacg cgatatgtac
    14401 atcggccacc ggctcggcgc aagtgagcag tacgggcggg caggggccgt acagttttgc
    14461 atggagccac gatgccgctc tgacgacggg gctggcgtcc aacctcgttg ccggcagcta
    14521 ttcggtcact gccacagatg cggagggatg tacggcccag ttgagcctga cagtcggcac
    14581 acaaaacgag cctccgcaat tggctttggt gcaaaccacc gacagcgatt gcaacggctc
    14641 cacaggcagc atagaagtga gcgctaccgg agcccaaggt ccctacactt tcgtctggag
    14701 ccatgatgca ggcttgaaca gcaacatcgc cggcaacctc tcggcggcag gatacacagt
    14761 gactgccacc gacgccaacg gctgcacccg aacggaggtg gctcaagggg gcatctttgc
    14821 cgaacccgac ctcggcacca cgcttgcgca cgtttcctgt gccaatgcac aggacggcag
    14881 cattgccgtg gggcttttcg gcggtac
//
LOCUS       DAORLF010000050        14760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2184_length_14760_cov_1.966270, whole genome shotgun sequence.
ACCESSION   DAORLF010000050 DAORLF010000000
VERSION     DAORLF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14760
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2184_length_14760_cov_1.966270"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PK198_06390"
     CDS             complement(<1..519)
                     /locus_tag="PK198_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF38395.1"
                     /translation="MKKTFAPDKRIAVWDVQTSVSGKTLAVSGVTDQPEAKGALLQRL
                     QQADIMAQTNLQILPDTALGAYIFGIVKVSVANIRSEPKHSAELATQALLGAPIKVLR
                     QQGDWYQIQTPDRYIGWLENGAFARKTEAEMQRWRRSERLIFLGDHALCYAGPEQSVP
                     LSDLSTGAIVEWN"
     gene            750..1307
                     /gene="rpsF"
                     /locus_tag="PK198_06395"
     CDS             750..1307
                     /gene="rpsF"
                     /locus_tag="PK198_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRF38396.1"
                     /translation="MKNYEVTFIVDPVLSGDEIEATAQTYVNYLEKVGCHIVHVDKMG
                     LRTLAYEIKRRSSGVYFCIEFQSPNGEFMDDFELALRRDERLLRFLTIALDKFGVKYN
                     DDKRNGLIGKKKRAGAETKEEAPAPVAAAPAPVETAAPVVTAEEFAAAAPAVVEEEVV
                     EPVVEVAAEAVEEAAEAATESEEQA"
     assembly_gap    1332..1426
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1427..1623
                     /gene="rpsR"
                     /locus_tag="PK198_06400"
     CDS             <1427..1623
                     /gene="rpsR"
                     /locus_tag="PK198_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRF38397.1"
                     /translation="FRKFGIKHIDYKDADFLLQFVNDQGKLLPRRITGNSLKFQRKIS
                     TAVKRARHLALLPFVTDMLK"
     gene            1653..2102
                     /gene="rplI"
                     /locus_tag="PK198_06405"
     CDS             1653..2102
                     /gene="rplI"
                     /locus_tag="PK198_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRF38398.1"
                     /translation="MDIILLQDIEKVGEKHTVVKVRDGYGRNYLIPQGMAIVANKQNM
                     NRLEGLKRREGAVEAQMITEYQEIAARLEGKTIQIGAKAGTSGKIFGSVTNVQIAAAL
                     KDQLNIDVDRRKISLPSEDVKMVGAYTAKLMLHKEVQPEFSFEVVAE"
     gene            complement(2188..2772)
                     /locus_tag="PK198_06410"
     CDS             complement(2188..2772)
                     /locus_tag="PK198_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38399.1"
                     /translation="MKYLCCLLLLLAAQFQIGKAQTQMMKSYLHGDWVLSHDYFSYDG
                     CDLGETPLALEAESFTLVSFSAFNDSFLIRRGKEILRKGLYTLTFAPLYEHIPHCTEV
                     VFIDLREDKNEKLAHSHHGDNWEYLILTPDMRIASKYYAPGMCGSWGYFSKIGWGFVN
                     ITCTTGHLRYMSNLIISRQQVHWVEKKKKQTEKR"
     gene            complement(2826..>3332)
                     /locus_tag="PK198_06415"
     CDS             complement(2826..>3332)
                     /locus_tag="PK198_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRF38400.1"
                     /translation="VFHPDRMAQRILGMGDIVSFVEKAQAQFDEKEIERLEKKIRKNQ
                     LDFNDFYSQIQQLRKMGDLKSLMGMIPGMGKALRDVDIDNSAFNRTEAIILSMTPTER
                     TNPDLINMSRKKRIADGSGNSLDQVNAFIRQFEQMRKMMHKMSKMPGMGMPAQMPGGA
                     AGAKKLRR"
     assembly_gap    3333..3426
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3427..4191)
                     /locus_tag="PK198_06420"
     CDS             complement(<3427..4191)
                     /locus_tag="PK198_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRF38401.1"
                     /translation="MFNNLTDRLDSAFKTLKGEHKLTELNVAESIKEIRRALVDADVN
                     YKIAKEFTDKVKEQALGTQNVLKAVKPGELMVKIVMDELVNLMGGEAAEFNVKGKPTV
                     ILIAGLQGSGKTTFSGKLANYLKSQLKKKPLLVACDVYRPAAIDQITVLGEQINVPVY
                     KEVENKNPVEIALKSIEHAKAHNLDVVIVDTAGRLAVDEEMMKEIAAVKGAIMPHETL
                     FVVDSMTGQDAVNTAKAFNEVIDYDGVVLTKLDGDTR"
     gene            4401..5249
                     /locus_tag="PK198_06425"
     CDS             4401..5249
                     /locus_tag="PK198_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF38402.1"
                     /translation="MSTPIKILLLLLAWLLYTVFAYRGCKEELCLHCGDGTAGVVAPP
                     AGPEESITRYPIDFQWDNAEAFKNDGADAELKALAAGLQNNNNVLEVTGYYYEGEKAP
                     PGFDNMGFARAARLKDMLAAAGVPADRIQTRARALQGGEDAQKGYFVGYEGNWKVVEQ
                     AAEVKTVEELDDRIIIRFPSGSTQKDYDKNVDDYLAKLVARIKETGETVTLSGHTDNV
                     GNADGNLKLGQGRADAVKRLLVKKGAPADQITTESKGQTQPTASNETPEGRHENRRVE
                     VRLNKK"
     gene            5340..6308
                     /locus_tag="PK198_06430"
     CDS             5340..6308
                     /locus_tag="PK198_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF38403.1"
                     /translation="MFTGNLLLHIETNCWLWWLLGSLLPLLLGIAIGYLLFSKYRRLA
                     AELEAERNQLKGEVAEWEKNYMSLKYQLDECQKERDRMKISLQSCEADKAVLQFKLDH
                     SHDHDHDAHGIVAGSVAALAPKGAAGLGALFTSDNLQIIEGIGPKIEELLHAAGINTW
                     ADLAKADFDRLRQILDEAGPSYRIHNPGTWGQQAALAAAGKWDELITLQKALDAGEDT
                     DGGESDSKLEKMAVKLLGFSMNPSDLKVVEGIGPKIEELLKADGIHNWSDLAAASADR
                     IQAVLDAAGDNFRLANPGTWPRQAAMAAAGEWVELKAYQNELQGGK"
     assembly_gap    6563..6585
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6660..>8749
                     /locus_tag="PK198_06435"
     CDS             6660..>8749
                     /locus_tag="PK198_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRF38404.1"
                     /translation="MYQTFALDINALRSRLTHAPMERTEAAKSRAPMVEIPLPDGEII
                     RFSVWESPIMEQALAKRYPGIRTYAGRAVHDPAMTLRFDLTPQGFHATLFTQQFGAVF
                     IDPLYQGQDQLYQIYAKKDFVPAAHKTFSCAFNHSHANEPDGNEPQRGKRFGDCQLRV
                     YRLALACTGEYAQFHGGTVPLVLGAMVTTMNRVNGVYEREFAVRMNLVANNDTLIFLN
                     AETDPFTNNDVGAMLGENQAIIDSLITPEKYDIGHAFGTGGGGVAGYGVVCNPNSKAI
                     GVTGGGAPVGDPFDIDYVAHEIGHQFAGSHTFRGCGNDNANDPTALEPGSGSTIMAYA
                     GICGDNVQSNSNDYFHGYNLEEMSNFITLGGGNTCGEQIPLDNLPPEVTSSGQSYVIP
                     IGTPFFLTAQATDPDDDPLSYCWEQFDDELSPQPPQPTNTGGPNFRTLTPSPSPTRYF
                     PALPALVNGGPFIWEVLPTVARSMNFRVSVRDNAPGGGCTDHGSVAITVSDVAGPFVV
                     LNPDTAGIRWTAGGQEIVRWDVANTQLAPVSCAEVDILLSTDGGLSYPTVLAAGVPNN
                     GLHAVDVPHLLSSSARVMVVCAGSIFFDISNNNFSIVEPAAGFVLHTEPNAINSCGLD
                     EMSISIGLDTTDGFEGRIGLEVSGLPEGVLAAFDQDTLDGGGQATLTISGLSAATAGI
                     YTIVVTGTGNGGSQS"
     assembly_gap    8750..8822
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8823..10234
                     /locus_tag="PK198_06440"
     CDS             <8823..10234
                     /locus_tag="PK198_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38405.1"
                     /translation="RLMPLLAWNDMPRADTYTLEIADNPEFVPVLLAQAGLTDIQYQI
                     RNNLPDGAVLYWRIRAANACGSGEYGPVYQFTTAAVACTTLVSSDVPLAISPDDTVTV
                     FATVLFPVPGIVTDVNIPVLKGTHDWINDLQFSLIGPSGTTSALLGPVCWDEDHFDIR
                     FDDAATTPYNTIPCPPTDGGLYRPRSPLSVFNGQDAEGAWKLQVFDSWAADGGELTDW
                     SLEVCYLPPPNAGCSLSVAAEVIATHCTPCTSNLALMVTGASGSTAFLWNDGSRDASR
                     QAVCEGSYTVMVVDSASCEVTLQVEVPPATEFLSIGATATPAQGGNNGSATATATGGI
                     APLTYLWNTGDTTATVQQLAAGIYTVTVTDANGCTATTEVVVEFVSGLGEVIGLNEFR
                     LSPNPTGGKVQAFIAFEQDEEVWVEIYSIDGRRLQRNTQHGQRIELLFDLTDQNDGVY
                     FISVRTARGSVTKPVVLAK"
     gene            complement(10318..10842)
                     /locus_tag="PK198_06445"
     CDS             complement(10318..10842)
                     /locus_tag="PK198_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF38406.1"
                     /translation="MATTKWVLDPAHSEIQFKVKHMMIATVTGSFDRFHVEVETEGDD
                     FSTASIQFSAETASVNTGSEQRDGHLRSADFFDVENHAALVFKAKSYSPANNQLQGDL
                     TIKGITHPVTMEAEFGGIGQDPWGNTKAGFTLSGKINRKDWDLNWNAALEAGGVLVSD
                     EVRLLGEIQLAKSN"
     gene            complement(10936..11532)
                     /locus_tag="PK198_06450"
     CDS             complement(10936..11532)
                     /locus_tag="PK198_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF38407.1"
                     /translation="MSLEDQHALLMSNIRRHIDPTAEEEAYFLSLLQTAQVKKRRFLL
                     QEGDVCRHSAFVTNGCLRSYTVDANGFEHILQFAPENWWIADMYSFISGQPGQLYIDA
                     LEDTELLLLSKNRQEQLYLEVPKFERFFRIIVENSLVAGRQRILDNMSQSAAQRYDQF
                     CRQYPSLIHRLPQKQIAAYIGVTPEFLSKLRGEKARRK"
     gene            complement(11716..>13924)
                     /locus_tag="PK198_06455"
     CDS             complement(11716..>13924)
                     /locus_tag="PK198_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF38408.1"
                     /translation="KFERLSQGLHSVVIRYIGFQNLEKSVSIKAGQNRLDYALQPEAL
                     ALGEVTVTAKRPLITQEEDKMIIDPEPIANTSTSTLEVLEKTPGIFVDQEGGIFLNSA
                     TPAVIYINGREQRMSARDIATILQSLPPGSVQKIEVLRTPSTKYDAASSGGIVNVVLK
                     KGVRIGRTGSLNMGMNQGIYGNQFIGVNLNDSGGKGSYYINTNYSRNDAVEDLKSSRE
                     LSAESILSQNARSRFPSDFGYVGYGFNLDPNERWGFNYDGRVNLGLRRSSAYNLNTIN
                     TLDNILLSENDNSTDNRTGFFSLQQDLGLIHKLDTIGSEWDTKFSYNYNNNNNEQDYS
                     IQSLRPLSGTLAGNGDNRQNRHFILLQSDLTYRFPKQGIKLETGFKSTWQNYQSRADY
                     FFNVNGGASQPDALRTNAFNYRENINAAYLQASKSLPGNFLLKTGVRMENTRMDGRQT
                     VPADTSFVINRTDWFPYAYLSRPIMKIASFSLQSYLIYRRTINRPGYQNLNPYIKIVD
                     PYLYETGNPALQPQFTDNIEANISFNDMPIFAIGRNYTRNIFSNVVYQDPQFPEVAVR
                     TFDNVGKNRETYFRIVGAIPPGGKYFFVVSAQYNLNDYNGLYENQPLSFSRGSWQLFT
                     FHSLNLTPNTKLTMNGFVMFKGQQNFYELDTFGQLNFGITQTMWKKKLSITLSARDVL
                     RTMVTRFNINQGSINVFGERYNDNQRIGLNLRYNFGMKKREDQQNMFSPGDGE"
     assembly_gap    13925..14022
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14240..14653)
                     /locus_tag="PK198_06460"
     CDS             complement(14240..14653)
                     /locus_tag="PK198_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRF38409.1"
                     /translation="MELSIQTPALLFPAISLLMLSYTNRFLAISALIRKLSADFESTE
                     HLGLLPQIKNLRRRVYLIRWAQFFAIISMLGCTLTMFLVYERLQSPATIVFAGSLLLM
                     MISLLLCLWEIFVSADALRLVLNDLEEKERALKKH"
BASE COUNT         3342 a         3652 c         3927 g         3456 t
ORIGIN      
        1 attccactcc acaatggcgc ccgtactcag gtcgctgaga ggaaccgact gctcagggcc
       61 tgcataacac aaggcatgat cgccgaggaa aatcaggcgt tccgaccgcc tccagcgctg
      121 catttccgct tcggttttac gggcaaatgc accgttttcc aaccagccta tatagcggtc
      181 gggggtttgt atctggtacc agtcgccttg ttggcgcagc actttgatcg gcgcacccag
      241 cagggcttgt gtggccaatt cggcgctgtg tttgggttcg gagcggatgt tggccaccga
      301 cacctttacg atgccgaaga tgtatgcgcc cagagccgta tccggcagta tttgcaggtt
      361 tgtctgcgcc atgatgtcgg cctgttggag cctttgcagt agcgcgccct tagcttccgg
      421 ctgatctgtg acaccagaaa cagcaagggt tttgccgctc acggaggttt gtacatccca
      481 cacggcgata cgcttatcgg gggcgaatgt ttttttcaag tcggcgagga tggaatctgc
      541 gacggcttgt tttgagattt gccctgttgc gctgccgtac aaacaaaaga ggagacaggt
      601 aagcatgatg gttcgcatat cgaagagatt gttggaaagc aaagatagca gcccggaata
      661 aaattttgtt tacagtatgt aaaaaatcga aagagataat tacctttgca cccgcttttt
      721 gattttttaa ccattatgtc tgataatcaa tgaaaaacta cgaagttact ttcatcgtgg
      781 acccggtgct gtcgggcgat gaaattgaag cgacagctca gacatatgtc aattacctcg
      841 agaaggtagg ttgtcatatt gtgcatgttg acaagatggg gctgcgtacg ctggcctatg
      901 aaatcaagcg ccgttcttcc ggcgtttatt tctgcatcga attccagtcg cccaacggcg
      961 aattcatgga cgactttgag ctggccctgc gccgcgacga gcgcttgctg cgcttcctca
     1021 ccattgctct ggacaagttc ggagtaaagt acaacgacga caagcgcaac ggcttgatcg
     1081 gcaagaagaa acgtgccggc gctgaaacca aagaagaggc tcctgcacct gtcgcagctg
     1141 cgcctgctcc tgtggaaact gccgctccgg tagtgactgc ggaagaattt gccgctgccg
     1201 cacctgctgt tgttgaagaa gaggttgttg aaccggtcgt tgaagtcgct gcggaagcag
     1261 tggaagaagc agccgaagca gctaccgaat cggaagagca agcctaacga aaccattctt
     1321 gtctaaccgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttt ccgcaagttc
     1441 gggatcaaac acattgacta caaagacgca gacttcctgc ttcaattcgt caacgatcag
     1501 ggcaaactgc tgccgcgccg catcacgggc aactcgctca agttccagcg caaaatttct
     1561 actgccgtca agcgcgcacg tcacctggct ttgctgcctt tcgtgaccga catgctcaag
     1621 taatgttttt ttggctctaa aatctcaaag aaatggacat cattcttttg caagatattg
     1681 aaaaagtcgg cgaaaagcac accgttgtca aagtacgcga tggctatggc cgcaattatt
     1741 tgatccctca gggtatggct attgtggcca acaagcaaaa catgaaccgc ttggagggac
     1801 tcaaacgccg cgaaggcgct gtggaggctc aaatgatcac tgaatatcag gagatcgctg
     1861 cccgtttgga aggaaaaacc atccaaatcg gcgctaaggc aggtaccagc ggcaagattt
     1921 tcggcagcgt gaccaacgta cagattgctg ctgcgctgaa agaccaactc aacattgacg
     1981 tggatcgccg caagatttca ttgccgtccg aagacgtgaa aatggtgggg gcttacaccg
     2041 ccaaactgat gttgcacaaa gaagttcagc ccgaattcag ttttgaagta gtggccgaat
     2101 aatcatctgc actttctaat cttctttcgc aggagccttt cccggtgtgg aacaggctcc
     2161 tgtgttgttt ttggggtaga gctggggtta tcgcttctca gtttgctttt tcttcttttc
     2221 aacccaatgt acttgttgtc ggctgatgat gagattggac atatagcgca gatgtccggt
     2281 agtacaggtg atgtttacaa agccccagcc tatttttgaa aaataccccc aggaaccaca
     2341 catccctggc gcataatact tagatgcgat tctcatatcc ggggttagaa tgaggtattc
     2401 ccaattatcg ccatgatgag aatgtgctaa tttttcgttt ttatcctctc tgagatcaat
     2461 aaaaacgacc tctgtacaat gtggtatgtg ctcgtacagc ggcgcgaatg tcaaagtgta
     2521 taaacctttg cgaagaattt ccttaccccg acgaatcaaa aaagaatcgt tgaaagcact
     2581 gaaagagacg agcgtgaaac tttccgcttc gagagcaagc ggggtctctc ccagatcaca
     2641 accatcataa gaaaagtaat cgtgactcaa aacccagtct ccatgaagat aactcttcat
     2701 catttgagtc tgagccttac cgatctgaaa ttgagcggcc agaagtagca aaaggcagca
     2761 taaatatttc atttagaata ttttagattg cagccattaa tctttgcact acgagtacgc
     2821 gccttctacc tcctcaattt cttagctccc gcagccccgc ccggcatctg tgcaggcata
     2881 cccatgcccg gcattttgct cattttgtgc atcattttgc gcatttgctc gaactggcgg
     2941 atgaaggcgt tgacctgatc gaggctgttg ccggagccgt cggcgatgcg ttttttgcgg
     3001 ctcatgttga tgagatcggg gttggtgcgc tcggtgggcg tcatggagag gatgatggcc
     3061 tcggtgcgat tgaaggcgga gttgtcaatg tccacatcgc ggagggcttt gcccatgccg
     3121 gggatcatgc ccatgagact tttgaggtcg cccattttgc ggagttgctg aatctggctg
     3181 tagaaatcgt tgaaatcgag ttgatttttg cggattttct tttcgaggcg ctcgatttct
     3241 ttttcatcaa actgagcctg cgctttttcc acgaaggaga cgatgtcgcc catgccgagg
     3301 atgcgttgcg ccatgcggtc ggggtggaac acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnngcgt gtatcgccgt cgagtttggt gagtacaacg ccgtcgtaat caatgacttc
     3481 attgaaggct ttggcggtgt ttaccgcatc ctggccggtc atggagtcca ccacaaaaag
     3541 ggtttcgtgc ggcatgatgg cgcctttgac ggcggcaatt tctttcatca tttcctcgtc
     3601 cacggcaagg cggccggcgg tgtccacgat aacgacatcg aggttgtggg ccttggcgtg
     3661 ttcgatggat ttgagggcga tttctacggg atttttgttt tctacctctt tataaacggg
     3721 tacgttgatc tgctcgccga gcacggtgat ctggtcaatg gcggcagggc gatacacgtc
     3781 gcaggccacg aggagcggct tctttttgag ctggcttttg aggtaattgg ccaatttgcc
     3841 ggagaaggtg gttttacccg aaccctgcaa acctgcgatg aggatgacgg tgggtttgcc
     3901 tttgacgttg aactcggcag cctctccgcc catgaggttg actaattcgt ccatgacgat
     3961 tttgaccatc aactcgccgg gcttcaccgc tttgagcacg ttttgagtgc cgagcgcctg
     4021 ttctttcacc ttgtcggtaa attccttggc aattttatag ttaacgtcgg cgtccaccaa
     4081 tgcacggcgg atttctttga tagattctgc aacgttgagt tcggtcagtt tgtgttcgcc
     4141 tttgagggtt ttaaaggcgg aatccaaacg gtcggtcaga ttattaaaca tgcgatttta
     4201 acattttttt gaaagcgatg gcaaaagtat gaaaagctct gcttattttt ggcctgttct
     4261 cctttcacgt cctgtgaaca tctttccgaa tcagcttgcc tgctttcgga ttatctgaaa
     4321 atgagtgcgt tatacgttcc cctattctat ttattgctga ttttcaggta aacattttta
     4381 actactcaaa acggaaatct atgtcaacac caattaaaat tctattgttg ctgttggcct
     4441 ggttgctcta caccgtcttc gcgtaccggg gatgcaaaga ggaactatgc ctgcactgcg
     4501 gcgacggaac tgccggcgtg gtggcgcctc ctgccgggcc ggaggagagc atcacgcgct
     4561 atcccattga ttttcagtgg gacaatgccg aagccttcaa aaacgatggc gctgacgccg
     4621 aactgaaagc actggccgcc gggctgcaaa acaacaacaa tgtgctggaa gtcaccgggt
     4681 attattacga aggcgaaaaa gcgccgccgg gcttcgacaa catgggcttc gcacgggctg
     4741 cccgcctgaa ggacatgctg gcagcagccg gcgtgcctgc cgaccgcatt cagacccgcg
     4801 cccgcgccct gcaaggcggc gaggatgcgc aaaaagggta ctttgtgggc tacgagggca
     4861 attggaaagt cgtcgagcag gctgccgagg tgaaaacggt ggaagaattg gacgaccgca
     4921 tcatcattcg tttcccatcg ggttctaccc aaaaagatta cgacaaaaac gtggacgact
     4981 atttggccaa attagtcgcc cgcatcaaag aaaccggcga aaccgtaacg ctctccggcc
     5041 ataccgacaa tgtgggcaac gccgacggca acttgaagct gggccagggc cgggcagatg
     5101 cggtgaaacg cctgttggtc aaaaaaggcg ctcctgccga tcaaatcacc accgaatcga
     5161 aaggacaaac acaacctacg gcctcgaacg aaacgccgga gggtcgccac gaaaatcgcc
     5221 gcgtggaggt aaggttgaat aagaagtaac tatgtgtatt cggctgcggg cctcaagctg
     5281 caagttgtgg gctgcaaaat caaaacaatc attgatccat cttaaaaaag aaaataacca
     5341 tgtttacagg aaatcttctc ttgcacatcg aaaccaattg ctggctgtgg tggctgctcg
     5401 gctccctgct gcccttgctg ctcggcatag ccatcgggta cctgcttttc agcaaatacc
     5461 gccgcctggc tgccgaactg gaagctgagc gcaatcaact caaaggcgaa gtagccgaat
     5521 gggagaaaaa ctacatgagc ctgaaatatc agctcgacga gtgccaaaaa gagcgcgacc
     5581 gcatgaaaat ttcgctgcaa agctgcgaag ccgacaaagc ggtgctgcaa ttcaaattgg
     5641 atcacagcca cgatcacgat cacgacgccc atggcatagt agccggttct gtggctgctc
     5701 tggctccgaa aggcgctgcc ggattgggcg ctttgtttac cagcgataac ctgcaaatta
     5761 ttgagggcat tggcccgaaa attgaggaat tgctgcacgc tgccggcatc aatacctggg
     5821 cagatttggc gaaagccgac ttcgaccgtc tgcgccaaat attggacgaa gccggtccga
     5881 gctaccgcat ccacaatccc ggcacctggg gccagcaagc tgctctggcg gctgccggca
     5941 agtgggacga actgattacg ctgcaaaagg cattggatgc cggcgaggat accgacggag
     6001 gagaatcgga ttccaagctg gaaaaaatgg ccgtaaaact gctgggcttt tccatgaatc
     6061 cgagcgacct gaaggtagtg gaaggcatcg gcccgaaaat tgaggaattg ctcaaggccg
     6121 acggcattca caactggtct gacctggccg cagcttccgc agatcgcata caagccgttc
     6181 tggatgctgc cggtgataac ttccgattgg ccaatccggg tacctggccg cgtcaggcgg
     6241 cgatggcggc tgccggcgaa tgggtggagc tgaaggctta ccaaaatgag ctgcaaggcg
     6301 ggaaataagt cttgctgaaa atgataaaaa ggcgtttcgt tcaagtgggc ggagcgcctt
     6361 tttttgttac ggcagaaaga taaaaatgca attgtctttc tcattgcaaa aactcaattc
     6421 cagcctgata agcctcacat ctttcttcta cttttagccc ctgaaaatca gcttacatca
     6481 tccaaacagc acatctacac atgcaccgtt tcctattact ctgcacactg cttttcccac
     6541 tcctcgcatc tgcccaattc ggnnnnnnnn nnnnnnnnnn nnnnnccgcc acactctgga
     6601 cgcccgcatc agcgtcagag acccggcagc cggcaggcga aagacgcatc caaccacaag
     6661 tgtatcaaac ctttgcctta gacatcaacg ctctgcgaag ccgcctgacc catgccccga
     6721 tggagcgcac ggaagccgcc aaaagccggg cgccgatggt ggaaataccg ctccccgacg
     6781 gcgaaatcat tcgcttcagc gtttgggaat cgcccattat ggagcaggct ctggcgaagc
     6841 gctatcccgg catccgcact tatgcgggcc gcgcagtaca cgatccggcc atgacactgc
     6901 gcttcgatct cacgccgcag ggctttcacg ccacgctgtt cacgcagcag ttcggcgcgg
     6961 tttttattga tccgctgtat caaggacaag accagttgta tcagatttat gccaaaaaag
     7021 acttcgttcc ggcggcgcac aaaaccttct cctgcgcctt taatcattca catgccaatg
     7081 agcccgacgg caacgagcct cagcgcggca agcgtttcgg cgactgtcag ttgcgcgttt
     7141 accggctggc cttggcctgc accggcgagt atgcacagtt tcacggcggc acggttcctt
     7201 tggtactggg tgctatggtt acgaccatga accgggtgaa cggcgtgtat gaacgggagt
     7261 ttgccgtgcg catgaactta gtggccaaca acgataccct catttttctg aatgccgaaa
     7321 ccgacccctt caccaacaac gatgtgggcg ccatgctggg cgagaaccaa gccatcatag
     7381 acagcctgat cactccggaa aagtacgaca tcgggcacgc attcggcacc ggcggcggcg
     7441 gcgtggcagg atacggcgtg gtgtgcaatc ccaactccaa ggccatcggc gtcacaggcg
     7501 gcggcgcgcc cgtcggcgac ccgttcgata tagattatgt ggcccacgaa atcgggcacc
     7561 aatttgctgg aagtcatacc tttagaggct gcggcaatga taatgccaac gaccccaccg
     7621 cattggaacc gggcagcggc tctaccatca tggcgtatgc gggcatttgc ggtgacaatg
     7681 tgcagtcgaa ctccaacgac tattttcacg gatacaattt ggaggaaatg tccaatttca
     7741 tcaccctggg cggaggtaac acctgcggcg aacaaatccc gttagacaac ctgccgcccg
     7801 aagtgacgag cagcgggcaa tcgtatgtta tccccatcgg aacgcccttt tttctcacgg
     7861 ctcaggcgac cgaccccgac gatgatccgc tcagctattg ctgggaacag tttgacgatg
     7921 agctaagtcc gcagccgccg caacccacca acaccggcgg ccccaatttc agaaccctca
     7981 cgccctcgcc gagtcctact cgctattttc cggccttgcc tgcattggtc aacggcgggc
     8041 cttttatctg ggaagtgctg ccgaccgtag cgcgcagcat gaatttccgc gtttcggtgc
     8101 gcgacaatgc ccccggcgga ggctgtaccg atcatggcag cgtagccatc accgtcagcg
     8161 atgtggccgg cccttttgtt gtactcaacc ccgatacagc cggcattcgc tggacggcag
     8221 gcgggcagga aattgtgcgc tgggacgtgg ccaacaccca actggcgccc gtgagttgcg
     8281 cggaagtgga catcctgctc tctaccgacg ggggcctctc ctaccccacc gtgctggctg
     8341 ccggcgtgcc gaataatggc ctccatgccg tggacgtgcc ccatctgctc agctcatcgg
     8401 ccagggtgat ggtagtttgt gccggaagca tctttttcga tatttccaac aacaatttca
     8461 gcatcgttga accggcggca ggatttgtgc tgcacaccga acccaacgcg attaattcct
     8521 gtggattgga tgagatgagc atttccatcg gcttagatac gacggatgga tttgagggtc
     8581 ggataggatt ggaagtttcc ggtttgccgg aaggcgtgct tgctgccttc gatcaggata
     8641 cccttgacgg cggcgggcag gccacattga ccatcagcgg attatctgcc gccacagcgg
     8701 gaatttacac cattgtagtg accggaaccg gcaacggagg aagtcagtcn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nncccggttg atgcccctgc tggcctggaa cgacatgcct cgcgccgata cttataccct
     8881 tgagatagcc gacaacccgg agtttgtacc ggtgcttttg gcgcaagccg gactgaccga
     8941 catccagtat caaataagaa acaacctgcc ggacggagca gtgctttact ggcgaatccg
     9001 ggccgccaac gcttgcggca gcggcgagta tgggcctgta tatcagttca ctacggcggc
     9061 ggtagcttgt acgaccttgg tgagcagcga tgtgccttta gccatcagtc ccgatgatac
     9121 cgtgacggtt tttgctaccg tcctgtttcc tgtaccgggt attgtgaccg atgtgaacat
     9181 acctgtactg aaagggacgc acgactggat caacgatctt cagttttcac tcatcggccc
     9241 atcggggact acctcggcac tcttggggcc tgtttgctgg gatgaggatc atttcgacat
     9301 ccgtttcgac gatgccgcca caacgcctta taataccata ccctgcccac ccacagacgg
     9361 cgggctttac cggccccggt cgccactgtc ggttttcaac gggcaagacg cggagggcgc
     9421 ctggaagttg caggtttttg actcttgggc tgccgacggc ggagaactga ccgactggtc
     9481 tttggaggtt tgctacctgc cgccacccaa tgccggctgc tcgctgagcg tcgccgctga
     9541 agtgatagcc acccattgca caccctgcac ctccaacctt gcgctgatgg taacgggcgc
     9601 aagcggctca actgcctttt tgtggaacga cggctcgcgg gatgcctcgc ggcaggctgt
     9661 ttgtgagggc agttacacgg taatggtggt ggactcggca agttgtgaag tgactttgca
     9721 ggtagaagta cctccagcaa ctgagtttct gagcatcggc gcaacggcca cacctgctca
     9781 gggtggcaac aacggcagcg ccacggcaac cgcaacggga ggcattgcgc cgcttactta
     9841 cctctggaat accggcgaca ctacggccac cgtgcaacag ttggcagccg gcatttacac
     9901 cgtcaccgtg acggatgcca atgggtgtac ggctacgacg gaagtggtgg tggaatttgt
     9961 ttcggggctg ggagaagtca tcgggctgaa cgaattccgg ctgtcgccca atcctaccgg
    10021 cgggaaagtt caggctttca tcgcctttga gcaagacgag gaggtatggg tggagattta
    10081 ctccattgac ggccgccgtt tacaacgcaa tacgcagcac gggcaacgca ttgaactgtt
    10141 gtttgacctg acggatcaga acgacggcgt atattttatt tcagtgcgaa cagcgcgggg
    10201 cagtgtgacg aagccggtgg tgctggccaa gtgaggctga tgtgacaatg tgttcaataa
    10261 aaaaaccggc aggacttcaa tgagaagatc tgccggtttc attttttata aaacggctca
    10321 attgcttttc gccaactgaa tctcgcccaa cagacgcact tcgtcgctga ccagcacgcc
    10381 gcctgcttcc agcgctgcgt tccagttgag gtcccagtct ttgcgattga ttttgcccga
    10441 aagggtgaag ccggccttgg tgttgcccca ggggtcctgg ccgatgccgc caaactccgc
    10501 ttccattgta acgggatgcg tgatgccttt gatggtcaga tcgccctgaa gttggttgtt
    10561 cgcaggcgaa taagatttgg ctttaaatac caatgctgcg tggttttcca catcgaagaa
    10621 atcggcgctg cgcaggtggc cgtcgcgctg ttcgctgcct gtgttgacag aggccgtttc
    10681 ggcagagaac tggatggagg ccgtagagaa gtcatctccc tcggtttcta cctctacatg
    10741 gaagcggtcg aagctgccgg tgacagtggc gatcatcatg tgcttgacct tgaattgaat
    10801 ttcagagtgt gcgggatcga gtacccattt tgttgttgcc atgttggttt tgattttact
    10861 ttattcaaaa gatggcacaa agatgggggc ggaatggggt gaaaaacatt gaagtagatt
    10921 aagaaatgca aaaagtcatt tccggcgggc tttttcgccg cgcaatttgc tcaaaaactc
    10981 aggcgtaacg ccgatataag cggcaatttg cttttgagga agccggtgaa tgagcgaagg
    11041 gtactgccgg caaaactgat cgtagcgctg tgcggcgctc tgactcatgt tgtctaaaat
    11101 gcgctgccga ccggccacca aagagttttc cacgatgatt ctgaaaaagc gctcaaattt
    11161 cggcacttca aggtacaatt gctcctgccg gtttttcgag agcagcaaca gttccgtgtc
    11221 ttccagcgca tcaatgtaca attgtcccgg ttgcccggaa ataaagctgt acatatcggc
    11281 gatccaccag ttttcggggg caaattgcag gatgtgttca aagccgttgg cgtccacggt
    11341 gtaactgcgc agacagccat tggtaacgaa ggccgaatga cggcacacat cgccttcctg
    11401 aagcagaaac cggcgttttt tcacttgcgc cgtttgcaac aaagataaaa aataagcctc
    11461 ctcttcggca gtggggtcaa tgtgccggcg gatgttgctc atcaggagcg cgtgttgatc
    11521 ttcgagactc atggatatat ctttttaatg tcatctgctg cgcagtttat caaaagtcat
    11581 ctgctgcgca gtttatcaaa agtcatctgc ttcgcagttt atcgaaagtc atctgcttca
    11641 cagtttatcg aaagtcatct gctgcgcagt tcatcgctgg atggaacccg aaactcggaa
    11701 cccgaaactc tcccctcact ccccatcgcc cggcgagaac atattttgtt gatcctctcg
    11761 ctttttcatg ccgaaattgt agcgcaaatt tagcccaatg cgctggttgt cgttgtagcg
    11821 ctcgccaaaa acattgatcg aaccctgatt gatgttgaag cgcgtgacca tcgtgcgcaa
    11881 cacatcgcgg gcactgagcg tgatactcag ttttttcttc cacatcgttt gagtgatgcc
    11941 aaaattgagc tgcccgaagg tgtctaattc atagaaattc tgctggcctt taaacattac
    12001 aaaaccattc atcgtgagct tggtgttcgg cgtgagatta agggagtgga aggtgaacaa
    12061 ctgccaactg ccccgcgaga agctgagcgg ctgattttca tacaatccat tgtaatcgtt
    12121 gaggttgtat tgggcgctga ccacaaaaaa gtacttgccg ccgggtggaa tagcaccgac
    12181 gatgcgaaag tatgtttctc tgtttttgcc cacattgtcg aaagtgcgca cggctacttc
    12241 ggggaattgc gggtcttgat acaccacatt ggaaaaaata ttgcgggtgt aattgcggcc
    12301 aatcgcgaag atgggcatgt cgttgaaact gatattggcc tctatgttgt cggtgaattg
    12361 aggttgcagg gccggattgc cggtttcgta caaatacggg tccacgattt taatgtaagg
    12421 gttcaagttt tgatagccgg gacgattgat ggtgcggcgg tagatgagat aactttgaag
    12481 agaaaaactt gcaattttca tgatggggcg actgagataa gcataaggaa accagtcggt
    12541 tctgttgata acaaagctcg tatcggcggg cacggtctgg cggccgtcca tgcgggtatt
    12601 ttccatacgc acgccggttt tgagcaggaa gttgccgggc agcgatttgg aggcttgcaa
    12661 atacgccgcg ttgatgtttt cgcggtaatt aaaggcattg gtgcgcaagg cgtcgggttg
    12721 agaagcgccg ccgttgacat tgaaaaaata atccgcccgg ctttggtagt tctgccaggt
    12781 gcttttgaaa cccgtttcca gcttgatacc ctgtttggga aagcgataag tgaggtcgga
    12841 ttgcaacaga atgaagtggc gattctggcg gttgtcgcca ttgccggcca aggtaccgga
    12901 gagcggccgc agcgattgaa tgctgtagtc ctgctcgttg ttgttgttgt tgtaattgta
    12961 actgaacttc gtatcccatt cagaaccaat ggtatcgagt ttatgaatca gtcccaggtc
    13021 ttgctgaaga ctgaaaaatc cggtgcgatt gtcggtgctg ttatcgtttt ccgaaaggag
    13081 aatattgtct aatgtgttga ttgtattgag attgtaggca gaggagcgcc gcaaccccag
    13141 attcaccctg ccgtcgtagt tgaaacccca gcgttcattg gggtcgagat tgaagccgta
    13201 gcccacataa ccgaaatcgg aaggaaaacg cgaacgggca ttttgagata aaatgctctc
    13261 cgcagacaat tcccggctcg atttcaggtc ttccaccgcg tcgttgcggc tgtaattggt
    13321 attgatataa tacgagcctt tgccgccgct gtcgttgagg ttgacgccga tgaactgatt
    13381 gccgtaaatg ccctggttca tacccatatt gagcgagccg gtgcgcccga tgcgaacgcc
    13441 ttttttgagc accacattga cgataccgcc cgaactggct gcgtcgtatt tggtagaagg
    13501 cgtgcgcaaa acctcaattt tttgtacgct tcccggcggc aggctttgca ggatggtggc
    13561 aatgtcgcgg gcgctcatac gctgttctct gccgttgatg taaatgaccg ccggcgtagc
    13621 gctgttgagg aaaatgccgc cttcctgatc tacaaaaatg ccgggcgttt tttccaatac
    13681 ttccaaagtg ctggtgctgg tattggcaat gggttcggga tcaataatca ttttgtcctc
    13741 ttcctgcgtg atgagcgggc gtttggccgt cacggtcact tctccgagcg cgagggcttc
    13801 gggttgcagg gcatagtcga ggcggttttg gccggctttg atggagacgg atttctccag
    13861 attctgaaaa ccgatgtacc ggatgacgac gctgtgcagg ccctgcgaga gtcgctcgaa
    13921 tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtttttt cgttgtcttt
    14041 gacgttgata aggagtgtgg attgggcctg agctgccgta atcaataaaa aagagagcgc
    14101 aaatgcgagt gttaacagag tagtggttgc tttcattcca aattttggtt ggtgttgaca
    14161 atgcagacaa agacacttga aatcaacaaa agatgtacgg gcaggcgatt ttgagttttt
    14221 gttaacagag gtcggccgtt caatgctttt tcaaggcgcg ttctttctct tccaagtcgt
    14281 tgagtaccaa ccgcaaagca tccgcggaaa cgaatatttc ccataagcaa agcagcaacg
    14341 aaatcatcat caacaacaag ctgcctgcaa atacgatggt agccgggctt tgcaggcgct
    14401 catacaccag aaacatagtc agcgtgcagc ccaacatgct gatgatggca aaaaactgcg
    14461 cccaacgtat gagatagacc cttcggcgaa ggtttttgat ttgaggcagc agccccaaat
    14521 gttctgtgct ttcaaagtct gccgatagtt tgcgaatcag ggccgagatg gccaaaaagc
    14581 gattggtgta agacaacatc agcagggaaa tggccggaaa cagcagagcg ggtgtttgta
    14641 tcgaaagttc catgcggcac acaacagcgg tcggcgggtc ttggttgagc gggtttgggg
    14701 ttcaaggttt cgggtttcat ccgccgtagc cttggcgaag gcgggttcaa ggtttcgggt
//
LOCUS       DAORLF010000051        14688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2195_length_14688_cov_2.106062, whole genome shotgun sequence.
ACCESSION   DAORLF010000051 DAORLF010000000
VERSION     DAORLF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14688
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2195_length_14688_cov_2.106062"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK198_06465"
     CDS             complement(<1..1234)
                     /locus_tag="PK198_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRF38410.1"
                     /translation="MTTKLISSTLLIIIGLSLCASLPASERWGFFGHRRINRLAVFTL
                     PLEMIGFYKKNIEYVTEHAVDPDKRRYATKHEAVRHYIDIDKWGKYPFHEVPRDWMDA
                     LAKYTDIYVVNSRGDTLRIAGEGCTEIAEDRWQYKAGQKVNATPPTVKAYRDFFFQSV
                     QPRYYDDEWPMPCDTLTALFGLPPGTCASAFAVDRFSEHGILPYHLLVMQRRLTEAFR
                     QGSTSAVLRLSAEIGHYIGDATVPLHTTENYNGQLTNQTGIHAFWESRLPELFADKEY
                     DFLVGKADYIRNPKDFYWNLVLESHAMLDSVLAVEKDLSRLFPQDRQYCYEERLDVLV
                     RTQCEEYAAAYHTRLDGQVEKRMRDAVHAIGSAWFTAWVDAGQPDLSKFGTDRLAQKE
                     KEEKEAIEAGLQKKEKKGR"
     gene            complement(1231..>1469)
                     /locus_tag="PK198_06470"
     CDS             complement(1231..>1469)
                     /locus_tag="PK198_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38411.1"
                     /translation="LVPFAVVKVTDDQKQVSEVRFHPIFNSGVYQDPKTGAYTSHSGA
                     ERYYANAVSGGDFFLVQHRVFQKIFWAYDSFFEP"
     assembly_gap    1470..1486
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1487..2253)
                     /locus_tag="PK198_06475"
     CDS             complement(<1487..2253)
                     /locus_tag="PK198_06475"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRF38412.1"
                     /translation="MNRTSILLILVLLLGAATFLYFQRGDDKTTLAGADRRFAVKNTD
                     DVYKIFIADRQGNKTTLERKDGYWLYNGRWRTRPTAIGNLLQTIGGVEMKFKPPHAAV
                     KNMVSSLAAEGIKVEIYDRKNKLLKSYYVGGATPDETGTYMILEGADQPYVTYLPGRQ
                     SNLRYRYSMKEDDWRDRSVFAYDPERIQAVSIEYPQQQNMSFRLERTGNKWNVKPFYD
                     FTPASARAYRERSVDTYVIGFESLVAEAFENQLPERDS"
     assembly_gap    2709..2725
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2726..3218
                     /locus_tag="PK198_06480"
     CDS             <2726..3218
                     /locus_tag="PK198_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRF38413.1"
                     /translation="KEAKGKRKLPVVIGGFEAQAIALAIENIQPSRPMTHDLFKNTME
                     SAGAELHEIIISDLSQGIFFATMVWELSTGALVEVDARTSDALAMAVRYNCPIFVNAE
                     ILYEAGIEMENPFFSSPGQAGGPQESSIEELQEKLDEALAQENYELAASIRDEIKRKL
                     EQQ"
     gene            3436..4833
                     /locus_tag="PK198_06485"
     CDS             3436..4833
                     /locus_tag="PK198_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRF38414.1"
                     /translation="MFQAATYQSRRSRLTQQLQGRGIVLLMGNEESPINYAGNVYRFR
                     QDSNFLYFIGLDQAHLAAVIDLDEGVTTLFGNDLSIEDVVWEGPQPTMLQLAARSGIE
                     KTAPYAALEEVLVKAKTAGRAIHFTPPYRAENKIRMSQWLGIAIDELKSAASEALIKA
                     IVEQRAHKTEEEVAEMELALDITFDMHMSVMLQAEPGLMESDLAGIAEAIAISRGGRP
                     AYGTILSMNGQTLHNPYYTNVLESGRMILGDFGAETGRHYAGDITRSFPVDKTFTPRQ
                     RDIYNIVLEAEMKAIEACGPGIRYADVHLLAARVIVEGMKAIGLMKGNTDDAVAQGAH
                     ALFFPHGLGHMIGLDVHDMEDLGEQYVGYGDMQRSTQFGTKYLRLARALEPGFVFTVE
                     PGIYFIPELIDLWRSEGKFTDFVNYDKLDDYRQFGGIRIEDNVLITDSGHRVLGPVVP
                     KTVEEIEEIRSSEPE"
     gene            4867..8118
                     /locus_tag="PK198_06490"
     CDS             4867..8118
                     /locus_tag="PK198_06490"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HRF38415.1"
                     /translation="MKALNPKEQIKDLLSRGDIEAAIDLFKEVLPGGSPLINDLILLE
                     GRYHTLQNQYLAAVIAPDAYLLQSNQITAGLLHLLDRWQNDAAELIQLALKSKAKFLD
                     LGNCSLSAIPDEIEQLTELEELNLGSVYWDDTRNQFAGSRNEGAANDLSSERFKGLLK
                     LKKLRKLSVRACNIRTLEGLDQLKQLHTLSVGQNDIKDIAPIAAMTNLKVLSLFGNNI
                     IDIKPLAALKKVEMLSINDNAVVDLSPLSQLHDLEELYASGNRIESIGEISALPKLKT
                     LNLDANLVKYVEPLAYMASLKTINLNSNPIIDCPPEIAFSGDVHLIQTYFQAKERSPQ
                     SGAAPDSELLDIEVELEEDQPVLEVKLILVGNSSAGKTSVSKLLRGLAFNAVESTTHG
                     INVTEWMVRAEEIADWRSTDMPPYRIKVNLWDFGGQEYYHGTHRVFLDSNAVYVLVWS
                     NDKNSNLVEMTRLYNEEGQIIEMPLEHFHYHYWLDNIRFHAPGNVTGDKPPIVLLENK
                     IDKSDGREHWYDRKILEQYNVNLTAAISAYYAQSDENNKRRYRYAFDIFREDLLRILV
                     KKAEANAKIANLPAEWVTVRDQIRNIRSATAQAGLVNPFSVMAKENAWLSFSDFTAAC
                     RKVRAELTDNEIEKLADFLNQTGVVVWMAHIDRQKIYIDPGWLTEKMYRVLNERVRLK
                     NGRFGFGDILPALNSDTAETTQMLNLMQEWRIIFRESNNTQDWIAPQYLPDEHPMENL
                     FTIALAGLQENFYMFRAPLFHVRRILRHLVLHFGKHDQVEAREYWKYGVLFVVRPPLN
                     DTGAEHERLRIFIKSLPDEKDSALGKLMVCIEPAKKLTEVWRENIFRELLATFNESLL
                     RSMATGSISGIPLREVAKQRDELFLSLNGQDFASIQRIVGDAEAGFSAVVSEERRRIP
                     MPEFSALLRAVEITPPAPSLFISYSHNDTELRKAMEIHLGALRRLGKVISWSDGQIIP
                     GQDWNEAILRNLRSADIVLLLVSPDSLDSDFIWKVEIAEAMKRQEQGKCRVIPVLLRP
                     CDWKEMAFSKLEVSPKDPRNARPKPISLWQDRDEAFSIVVQGIKRALKELAVV"
     assembly_gap    8675..8691
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8692..10209
                     /gene="ade"
                     /locus_tag="PK198_06495"
     CDS             <8692..10209
                     /gene="ade"
                     /locus_tag="PK198_06495"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRF38416.1"
                     /translation="EGQPWIMPGFVDAHIHIESSMLVPSEFARMAVVHGTVATVSDPH
                     EIANVLGMEGVQYMIDNGQKVPFKFFFGAPSCVPATAFETAGASLNAEEVARLLAMPE
                     IRYLSEMMNYPGVLFSDPEVMAKIAAAKALGKPVDGHAPGLRGEQARNYFAAGISTDH
                     ECFTYEEGLEKAQLGVNIIIREGSAAKNFEALIPLLPQFPGQVFFCSDDKHPDDLILG
                     HINLLVLRALQKGCDLFDVLQAACINPVRHYGLNVGLLQPGDPADFIVVEDLQSFRVL
                     ATYIEGQITAEEGRSLIERIEAPVINRFNTSPKRPADFRLQALSNQVRYIEAVNGEIV
                     TRSGTAPIPVAEGWAVAYAQEDLLKITVVNRYEDAPPAVAFIRGFGLQKGAIASCVGH
                     DSHNIIAVGTDDESLCAAVNAVIASTGGISAVGAGREMVLPLPVAGIMTQADGYETAR
                     AYAEIDAWVKSELGCRLTAPFMTLSFMALLVIPELKLSDKGLFDGAAFAFAEVFA"
     gene            10264..>11469
                     /locus_tag="PK198_06500"
     CDS             10264..>11469
                     /locus_tag="PK198_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38417.1"
                     /translation="MALQKLPIGIQDFRTIIEDGYKYVDKTRFIHQLVTNGKFFFLSR
                     PRRFGKSITIATLQELYSGSRELFKGLWIEDKWDWNKRHPVIRISFTSIGHYTLGLPA
                     ALEQELRTQAQKMGFTLSKVGIGPMFEELIELTAREAKAVILIDEYDAPIIHYLGKEI
                     ETAYQNREILKEFYTVLKDKDALVEFVFLTGVSKFSKVGIFSGLNNLQDISLHPAFAN
                     MLGYTQQELEGNFAEEITAAAEQMRLSREELLAKMKYWYNGYRFEECADSVYNPVSCH
                     SFFSQNKFENFWFATGTPTFLINLLKQQGLYDFKLTEQSQLEFDSFDLEDLRPYGLLY
                     QTGYLTIIDRDEAGIYQLGYPNYEVENSMLAYLLDAFGGVPKGSGLVVALRLERAFES
                     GDLEQVMRIL"
     assembly_gap    11470..11703
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11881..13116
                     /locus_tag="PK198_06505"
     CDS             11881..13116
                     /locus_tag="PK198_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF38418.1"
                     /translation="MTQDTRFTSYQIFIIGTLALLQFAIVLDFMVMSPLSAQLMPSLK
                     INPTQFGLVVSAYAFSAGASGLLAAGFADKFDRKRLLLFFMAGFLIGTLLCGLAPNYP
                     FLLGARIVTGLFGGVIGSVSFAIITDLFEVQQRGRVMGFVQMAFAVSQVLGLPVGLYL
                     SNIWDWHAPFLMIVVVATPLWFVIAMRMRPVNAHLALQKKQSAAGHLLATVSKPFYLR
                     AFAATTLLATGGFMLMPFGAAFAVNNLGIGMDDLPVIYLATGVVSMIAGPLIGKLTDR
                     YSPMPLFLAGTILSIVLVLVYTHLGITPLWALIVLNAVLFVGITARMISSSTLVTSVP
                     TTADRGAFMGVNSSVAQISGGIASATAGLIIHQTESGMLENYPLLGWVVATSMVICAL
                     LFWGITKAVRKRDRALAKG"
     gene            13262..>14688
                     /locus_tag="PK198_06510"
     CDS             13262..>14688
                     /locus_tag="PK198_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38419.1"
                     /translation="MRKFTHLISLIFCCLCFVYVAQGQPVSVSNPSNCGLQILLSDNN
                     CPENNPNIYQPDEIGIQVVGQPGTALGIDVYLKEVRIILEHTWMSDMNLSLVSPGGRE
                     VLLIANQGGSGDHIGNPVDVSCMQFATFSAGACTPISAGAPPYTDQAYLPIQSLLGFN
                     DSITAPNGTWLLRICDDVPNDVGSLQYVELVFEPVSCLPIQQKQVLSVDSTTVVLDWS
                     PVGDCNNFIIEYGAPGFVPGTGADGGEGQTEAVIICPPYPLMDLLPDTEYDIYIRRFC
                     VGGGFSGNGCPIRVRTGCQPPPTTIVETFDAATPCFPNCGAVCNINGFWRNAGSDDFD
                     WLVYQGATPTPNTGPLAGFNDAGRYVYIETSGVLCTNGKRAILESNCVRLRKFNTDTC
                     HLSFAYHMWGVNINTLQLQVSSDGGFSWNTLWQRTGSQGNGWKKAYIGLGTYPEGAVL
                     KFRFIATGGNGSLGDIALDQIVFHGS"
BASE COUNT         3322 a         3917 c         3858 g         3306 t
ORIGIN      
        1 cccgcccctt cttttctttt ttctgcaagc cggcttctat ggcttctttt tcctcttttt
       61 ctttttgcgc cagccgatct gtgccaaact tcgataaatc aggctgcccg gcgtccaccc
      121 aggccgtaaa ccaggcgctt ccgatggcgt gtacggcatc ccgcattcgc ttctctacct
      181 gcccatccaa tcgggtatga tatgcggcgg cgtattcctc gcattgcgtg cgcaccagca
      241 catccaatcg ctcctcatag caatattgac gatcctgcgg gaataaccgg ctcaggtctt
      301 tttctactgc cagcacgctg tcgagcatgg cgtggctttc caaaacgaga ttccaataga
      361 agtctttcgg gttgcgaatg taatcggctt ttcctaccag aaaatcgtac tccttatcgg
      421 caaacaattc cggcaaacgg ctttcccaaa aagcgtgaat gcccgtttgg ttggtcagtt
      481 gcccgttgta gttttctgta gtgtgcagtg gcacggtagc atcgcctatg tagtggccta
      541 tttctgccga aaggcgcaac accgcggatg tactgccttg ccggaaagcc tcggtgagcc
      601 gccgttgcat caccagcaaa tgatagggta aaatgccgtg ctctgaaaag cgatccactg
      661 caaaagccga agcgcaagtt ccgggaggca acccaaacaa agccgtcagg gtgtcgcagg
      721 gcatgggcca ctcgtcgtcg taatagcgcg gctgcaccga ttggaagaaa aaatcgcggt
      781 aggctttgac cgtgggcggc gtggcgttta ctttttggcc ggccttgtat tgccagcggt
      841 cttccgcaat ttccgtgcag ccctcgcccg caatgcgcag agtatcgccc cggctgttca
      901 ccacataaat gtcggtgtat ttggccagcg catccatcca gtcgcggggc acttcatgga
      961 aggggtattt gccccatttg tcaatatcaa tgtagtggcg aacggcctcg tgtttggtgg
     1021 catagcgtcg cttatcgggg tcaacggcat gctcggtgac gtactcaata ttttttttat
     1081 aaaaaccgat catttccaac ggcaaagtga acaccgccag ccggttgatg cgccggtgtc
     1141 cgaagaagcc ccaacgttcg gaagccggca gggatgcgca caagctcaat ccgatgataa
     1201 tgagcaaagt agaagagata agtttggttg tcatggttca aaaaatgaat cgtatgccca
     1261 gaatattttt tgaaaaaccc tgtgttgtac taaaaagaaa tcgcctcccg acacagcgtt
     1321 ggcatagtag cgttcggcgc cggaatggga tgtgtaagct ccggttttgg ggtcctgata
     1381 caccccggaa ttgaaaatgg gatggaacct tacctcggaa acctgctttt ggtcgtcggt
     1441 cactttcacg acggcaaagg gaaccagggn nnnnnnnnnn nnnnnngaat cgcgttcggg
     1501 gagttgattt tcaaatgcct ctgcaacaag actttcaaaa ccaatgacat aagtgtcgac
     1561 gcttcgttcc cggtaggcgc gcgctgaggc gggcgtaaag tcgtaaaagg gcttgacgtt
     1621 ccatttatta cctgttcgtt ccagccggaa actcatgttc tgctgttgcg gatactcgat
     1681 actcacggcc tgtatgcgct ccgggtcata ggcaaaaacc gagcggtcgc gccagtcgtc
     1741 ttctttcatg ctgtagcgat agcggagatt gctttggcgg ccgggcaggt aggtgacata
     1801 aggttggtcg gcgccctcca gaatcatgta agtgcctgtt tcatcgggag ttgcaccgcc
     1861 gacgtaatac gatttgagca gcttgttttt gcggtcgtaa atttctactt tgataccttc
     1921 agcggccaag ctggaaacca tgttttttac ggcggcatgt ggcggtttga atttcatttc
     1981 cacgccgccg atggtttgca ggaggttgcc gatggcggtg ggccgggtgc gccagcggcc
     2041 attgtagagc cagtagccgt ctttgcgctc cagcgtggtt ttgttgccct ggcggtcggc
     2101 gatgaaaatc ttatagacat cgtcggtgtt tttcactgcg aaacggcggt cggcgccggc
     2161 aagtgtggtt ttgtcgtctc cgcgctgaaa atacaggaaa gtggcagcgc caagcaagag
     2221 tacgaggatc aataaaatgg atgttctgtt cataatatgc aatgtcggac ggcttttaaa
     2281 agcttggttg ttaaggagaa tcaggccgtc gcggggcgaa aatagcgatt tttcatcaga
     2341 cgcgaacaac gcgttgcaac ggaacagcat acccttgttg ggcaaagcgt gttccgagcc
     2401 tcaaaacacc gaaaaataaa atcatccatc tcattctgca aagctcgcca ttgaacttga
     2461 gaaacggctg atttcgttta tttttgctca gctgttattg gcaggtattt atgggagcat
     2521 tgtcgttcat tgtccaaaaa ttccgatatt gcgcccgctt tcttccgttt cacaacctga
     2581 aaggcggtta tcggaaatga aagctaagcc gcagcatcat ggcggcgcga tccaaaaaca
     2641 ttgtcatgca aaaaatagag cttcacattc tggccattgc caacagtgaa aaccaaccgc
     2701 agagctttnn nnnnnnnnnn nnnnncaaag aagcgaaagg caaaaggaaa ctgcccgtag
     2761 tcatcggagg ctttgaggct caggccattg cgttggctat agagaacata cagcccagcc
     2821 gcccgatgac ccacgatctg ttcaaaaaca cgatggaaag cgccggggcc gagctgcacg
     2881 aaatcatcat atccgatctt tcgcagggta tttttttcgc tacgatggtg tgggagcttt
     2941 ctaccggcgc gctggtcgaa gtggatgccc gtacctccga cgcgctggcc atggcggtgc
     3001 gctacaattg cccgattttc gtcaatgctg aaatcctcta cgaggccggc atcgaaatgg
     3061 aaaatccttt tttctcttcg cccggacagg caggcggccc acaggaatcc agtatcgaag
     3121 aattgcagga aaaattggac gaggcgctcg ctcaggaaaa ctacgaactg gcggcttcta
     3181 tccgcgatga aatcaaacgg aaattggagc agcagtaggg ccagtcaacg agccgcttgg
     3241 cttttagccc ttagctattt gctgctggcc ggatattcct caatagaggc agttttttca
     3301 tgtggggaaa aatatcaaag ctcgattttg aaagaaaaac atctgaatat ttgaaagcaa
     3361 agctaatagc cagcagccaa cagctaatgg cctacttctc gctccattga ggaataacca
     3421 aaataaaacc aactcatgtt tcaagcagcc acttaccaat cccgccgcag ccgactgaca
     3481 caacagcttc agggccgggg catcgtgctt ttgatgggca atgaagaatc gccgatcaac
     3541 tacgccggca atgtatatcg ctttcggcaa gacagcaact tcctctattt cataggttta
     3601 gaccaggcgc atctggccgc agtcattgat ctggacgaag gtgtgaccac gcttttcggc
     3661 aatgacctca gcattgaaga cgtcgtttgg gaagggccgc aacccaccat gttgcaattg
     3721 gccgctcgct ccggcatcga aaaaacagcc ccttatgcag ccttggagga ggtattggtc
     3781 aaagccaaaa ctgccggtcg cgccatccat tttacgccgc cataccgggc cgaaaacaaa
     3841 atacgcatga gccaatggct cggcattgcc atagacgaac tgaaatctgc cgcctccgaa
     3901 gcgctcatca aagccattgt ggagcaacgc gcccacaaaa ccgaggagga agtagccgaa
     3961 atggaactgg cgctcgacat cactttcgat atgcacatgt cggtcatgtt acaagccgag
     4021 ccgggcctta tggagtccga tctggccggc attgcagaag ccatcgccat atcgcgcggc
     4081 ggccggccgg cctacggaac catcctttcg atgaacggcc aaacgcttca caatccctac
     4141 tataccaatg tattggaaag cggtcgtatg attttgggcg atttcggggc ggaaaccggt
     4201 cgtcattatg ccggcgacat tacccgctct tttccggtgg ataaaacctt cacaccccgg
     4261 cagcgcgaca tttacaacat tgtgctggaa gccgaaatga aggccatcga agcctgcggc
     4321 cccggcattc gctatgccga tgtgcatctg ctggcggccc gcgtcattgt agagggcatg
     4381 aaagccatcg ggctgatgaa aggcaatacc gacgacgccg tagcacaggg cgcccatgcg
     4441 ttgttttttc cgcacggcct gggccacatg atcggcctcg acgtacacga tatggaagac
     4501 ctcggcgagc aatacgtcgg ctacggcgat atgcagcgca gcacgcagtt cggcaccaaa
     4561 tacctccgct tggcgcgcgc attggagccg ggttttgtct ttaccgtaga gcctggcatt
     4621 tatttcatac ccgaattgat tgacctgtgg cgcagcgagg gcaagtttac cgactttgtc
     4681 aattacgata aattggacga ctaccgtcaa ttcggcggca tccgcatcga agacaacgta
     4741 ctcatcaccg actccggcca tcgcgtactg gggcctgtcg ttccgaaaac ggtggaagaa
     4801 atagaggaaa taaggtcgtc tgaaccggaa tagaccttgc ggtttcgctc aaaaacctaa
     4861 atagccatga aagcactcaa tccgaaagag cagatcaaag acctcctctc gcgtggagac
     4921 attgaggcgg caatagattt gttcaaggaa gtgttgcccg gcgggtcgcc gctcatcaac
     4981 gatcttatct tgttggaagg tcgctatcat acgcttcaaa atcaatacct tgcagccgtc
     5041 attgcgcccg acgcatatct cctgcaatcc aaccaaatca ccgcgggcct gcttcacctt
     5101 ctggatcgct ggcaaaacga cgccgccgag ctgatccaat tggctctcaa gtccaaagcc
     5161 aaattcttgg atctcggcaa ctgctccctc agcgccattc ccgatgaaat tgaacagttg
     5221 acggagttgg aagaactcaa tctcggcagc gtgtattggg acgacacccg caatcagttt
     5281 gccggctccc gcaatgaagg cgccgccaac gacctctctt ccgagcgctt caaaggattg
     5341 ctgaaactca aaaaactgcg caaactctcc gtccgggcct gcaacatccg aacgcttgag
     5401 ggattggacc aactcaagca actccacacg ctttccgtcg gtcaaaacga catcaaagac
     5461 attgcgccca ttgccgcgat gaccaacctg aaggtgctgt ctttgtttgg caacaacatc
     5521 atagacatca agccgctggc tgccctgaaa aaagtagaga tgctctccat caacgacaat
     5581 gcggtggttg atctgtctcc tttgagccaa ctgcacgatc tggaagaact ctacgcgtcg
     5641 ggcaaccgca tcgaatccat aggcgaaatc tcggcgctgc ccaaactaaa aaccctcaac
     5701 ttagacgcca acctcgtcaa atatgtggag ccgctggcct acatggcctc tctgaaaacg
     5761 atcaatctca actccaatcc cattattgac tgccctccgg aaattgcctt ctccggcgat
     5821 gttcatctga tccaaaccta ctttcaggcc aaagaacgct cgccccaaag cggcgctgcg
     5881 cccgacagtg aactcttgga cattgaggtg gaactggaag aagatcagcc ggttctggag
     5941 gtaaaactca tcctcgtggg caacagcagc gcgggcaaga cctccgtttc caaactcctg
     6001 cggggcctgg cattcaacgc ggtggaaagc accacgcacg gcatcaacgt gacggaatgg
     6061 atggtgcgcg ctgaggaaat tgccgactgg cgctctaccg atatgccgcc ctatcgcatc
     6121 aaagtcaacc tctgggactt cggcgggcag gagtattacc acggcacgca ccgggtcttt
     6181 ttggacagca acgcggtgta tgtactcgtg tggagcaacg acaaaaactc caatctggtg
     6241 gaaatgacgc gtttgtacaa cgaagaaggc cagattatag aaatgccgct cgaacatttt
     6301 cattaccact actggttaga caacattcgt ttccacgcgc cgggcaacgt caccggagac
     6361 aagccgccca tcgttttgct ggagaataaa attgacaaaa gcgacggccg cgaacactgg
     6421 tacgaccgca agattctgga gcaatacaac gtgaacctca cggctgccat cagtgcctac
     6481 tacgcccaat cggacgagaa caacaagcgc cgttatcgct atgctttcga tattttccgc
     6541 gaagacctgc tgcgtatttt ggtaaaaaaa gccgaggcca atgccaaaat cgccaacctg
     6601 ccggcagaat gggtcactgt cagagatcaa atcagaaaca tacgctcggc aactgctcag
     6661 gcgggactgg tcaacccgtt ttcggtcatg gccaaagaaa atgcctggct ctcgttcagc
     6721 gattttacgg cggcctgccg gaaagtacgc gccgaactga cggataatga aatagaaaaa
     6781 cttgctgatt ttttaaatca aaccggcgtc gtggtttgga tggcccatat agaccggcag
     6841 aaaatttaca ttgatccggg ttggctcacc gaaaaaatgt accgggtgct gaacgagcgg
     6901 gtgcgcctga aaaatggtcg cttcggattt ggagacatcc tgcccgctct gaacagcgat
     6961 accgcagaaa ccacgcagat gctgaatctg atgcaggaat ggcgaatcat tttcagggaa
     7021 agcaacaaca cgcaagactg gatagcgccc caatacctgc ccgacgagca cccgatggaa
     7081 aacctcttca ccattgcgct ggcgggtttg caggagaatt tttacatgtt ccgggcgccg
     7141 ctgtttcatg tccgccgcat attgaggcat ttggtactgc attttggcaa acacgatcag
     7201 gtagaggccc gcgaatactg gaagtacggc gtcctgtttg tagtgcgccc gccgctgaac
     7261 gatacgggcg cggagcacga gcggcttcgc atttttatca aaagcctgcc cgatgaaaaa
     7321 gattctgcgc tcggtaagct catggtgtgc attgagccgg cgaaaaaact gaccgaggtg
     7381 tggcgggaaa acattttccg cgagctattg gccaccttca acgagtcttt gctcagaagc
     7441 atggctaccg gctccatttc gggcatcccc ttgcgggaag tggccaagca aagagacgag
     7501 ttgtttctct cgctcaacgg gcaggacttt gcctccatac agcgcattgt gggcgatgcg
     7561 gaggctggtt tttctgctgt ggtgagcgaa gagcgccgcc gcattcccat gccggagttt
     7621 tcggcgttgc tgcgcgcggt ggaaattacg ccgcccgcgc cctctctttt catttcctat
     7681 tcgcacaacg atacggaact gcgcaaggcg atggaaatcc acctcggagc gctccggcgt
     7741 ctgggcaagg tcatttcctg gtcggacggg cagatcattc ccggtcagga ctggaacgag
     7801 gccatcctgc ggaacctgcg cagcgcagac attgtgctgt tgctcgtcag cccggattcg
     7861 ttggactccg acttcatctg gaaagtggaa attgcggagg ccatgaaacg gcaggagcag
     7921 ggcaaatgcc gcgtcatacc ggtgctgctg cgtccctgcg actggaagga aatggctttt
     7981 tccaaattgg aagtctcgcc caaagacccg cgcaatgccc ggcccaaacc catctcgctg
     8041 tggcaagacc gcgacgaggc gttcagcatt gtggttcaag gcattaagag ggcgctgaag
     8101 gaactggcgg tggtgtgagt ttttcggtag gcggtgggca gtgggcggta ggcggcgctg
     8161 agcgcgaggg ttcggtaggc ggtttttcgg tgggcggttt ttcggtaggc ggtggccgcg
     8221 cctgtggcgc ggggtgcttg gcgcgagtga acgcggaact ttgaacgcga aacccggaac
     8281 ttgagggctg gccgcggcgg tatcaggcga catgaatgta aacacaagcg aggtgacatc
     8341 taacgccctg ctgcatcaac cggagggcgg caggtgtcat ctcgcggcta cgaaggaaac
     8401 cctcctctgt gttttcatgc gacgccttcc cgcttcgcca aagctcgcgg ttagatgtca
     8461 cctgaaaacg ccggcggcta cgaagaagaa tctccctccg ttttcatgcc gcaccttccc
     8521 gcttcgcaat ccgaaattag aaatccgaaa ttagaaatcc gatacaacca tgacactcac
     8581 cgtcaacatc ctccacatcc aggaacgcag cagcagcttc ggcgccgtcc acattgaaaa
     8641 cggccacatc acgcacattg agcacctcgg cccgnnnnnn nnnnnnnnnn ngaagggcag
     8701 ccctggatca tgcccggctt cgtggacgcg cacatccaca tcgaaagctc catgctggtg
     8761 ccttccgaat ttgcccgcat ggcagtggta cacggcacag tggccaccgt gtccgatccg
     8821 cacgaaatcg ccaatgtgct cggcatggaa ggcgtgcaat acatgataga caacggccaa
     8881 aaagtgccct tcaaattctt cttcggagcg ccctcctgcg tgcccgccac cgcgtttgaa
     8941 accgccgggg catcgctcaa cgcggaagag gtggcccggc ttctcgccat gcccgaaatc
     9001 cgctacctct ccgaaatgat gaactacccc ggcgtcctct tctccgaccc ggaagtgatg
     9061 gccaaaatag ccgccgccaa agccttgggc aaacccgtgg acggccatgc gcccggtctg
     9121 cgcggcgagc aggcccgcaa ctacttcgct gccggcatca gcaccgacca cgagtgtttt
     9181 acctacgagg agggtttgga aaaagcgcaa ctcggcgtca acatcatcat tcgcgagggc
     9241 agcgccgcca aaaacttcga agcgctcatt ccactgctgc cgcaattccc cggtcaggtc
     9301 tttttctgct ccgacgacaa acaccccgac gatctcattt tggggcatat caacctactc
     9361 gtgctcaggg ccttgcaaaa aggctgcgac ctgttcgacg tgttgcaggc ggcctgcatc
     9421 aatccggtgc ggcactacgg cctcaacgtc ggcctgcttc agcccggcga cccggccgat
     9481 ttcatcgtgg tagaagactt gcagtcgttt cgggtgctgg ctacctacat cgaagggcaa
     9541 ataactgccg aggaaggccg ctcgctcatc gaacgcattg aggcgcccgt catcaaccgg
     9601 ttcaatacct cgcccaagcg cccggccgac ttccgtttgc aggcgctcag caaccaggtt
     9661 cgctacatcg aggccgtcaa cggagagatc gtcacccgca gcggcacagc gcccattccg
     9721 gtggcagagg gctgggctgt ggcctatgca caggaagacc tgctcaaaat caccgttgtc
     9781 aatcgctacg aggatgcgcc gccggcagtg gccttcattc gcggtttcgg attgcaaaaa
     9841 ggggccattg cctcctgtgt cggccatgat tctcacaaca tcatcgccgt tgggacagac
     9901 gacgaatcct tgtgtgccgc cgtcaatgcc gtgatcgctt ccaccggagg catttcggca
     9961 gtgggagcgg gccgcgagat ggtgctgccc ttgccggtag cgggcattat gacccaggcc
    10021 gacggctacg aaaccgcccg ggcttacgcc gaaatagacg cctgggtgaa atcggagttg
    10081 ggttgccggc tcaccgcgcc gtttatgacg ctgtcgttca tggccttgtt ggtcataccc
    10141 gaattgaagt tgagcgataa gggcctgttt gacggagcgg cgtttgcgtt cgcggaggtg
    10201 tttgcgtaga gcgccgccgt tttgttgcag agtttcagta tcttcgcccg taaattgttg
    10261 taaatggcac ttcagaagct acccatcggg atacaggatt ttcgcaccat catagaagac
    10321 ggctacaagt atgtggacaa gacccgcttt atccatcagc ttgtcactaa tggtaaattt
    10381 ttcttcctct cccgcccgcg ccgtttcggc aaatccatca ccattgccac tttgcaggag
    10441 ttgtattccg gcagccggga gctgttcaaa ggcttgtgga tagaagataa atgggattgg
    10501 aacaagcgtc atccggtcat tcgcatttct tttacctcca taggacacta cacgctgggg
    10561 ctgccggcgg cattggagca ggaacttcgc acacaggccc aaaaaatggg gtttacgctc
    10621 tccaaagtcg gcatcgggcc tatgtttgaa gagttgattg aactcactgc ccgagaagcc
    10681 aaagccgtca tcctcataga cgaatacgac gcgcccatca ttcattatct tggcaaggaa
    10741 atagaaacag cttaccaaaa ccgagaaatt ttaaaggagt tttacaccgt tttgaaagac
    10801 aaggatgcac tcgtagaatt tgtcttcctc accggcgtca gcaaattttc caaagtgggc
    10861 attttctccg gcctcaacaa tctgcaagac atcagcctgc acccggcttt cgccaatatg
    10921 ctgggctaca cgcaacagga attggaggga aattttgcag aagaaatcac tgccgccgca
    10981 gaacagatgc gcctcagccg ggaagaattg ttggccaaaa tgaagtactg gtacaatggt
    11041 taccggtttg aggaatgtgc agattcggtg tacaacccgg tttcctgcca ctcttttttt
    11101 tctcaaaata aatttgaaaa cttctggttt gctaccggca cgcctacctt tctcatcaac
    11161 ctgcttaaac aacaggggct gtacgatttc aaactcaccg agcaatcgca gttggagttc
    11221 gacagcttcg atctggaaga cctgcgcccc tacggcctcc tctaccaaac cggctacctg
    11281 acgatcattg accgcgacga ggccggcatt tatcaactcg gctaccccaa ctacgaagtc
    11341 gaaaactcta tgctggccta ccttttagat gccttcggcg gcgtgcccaa aggttccggc
    11401 ttggtagtag ctttgcgttt ggagcgtgcc tttgaaagcg gcgatctgga gcaggtgatg
    11461 cgcattttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnaccatca ggccttctgg aatctgggca aaccggtggt gggcatcggc gtgaatttca
    11761 gcagcacgac ccgaaatatg gaggagtgga aggcggagga gatggcgatg acatagagcg
    11821 aagaaccgat ttccggcccc gaacgtatca tattccaaca ttcagagcac ccgatttttt
    11881 atgacacaag atacccgttt cacctcttac cagatattca tcattggtac gctggcgctg
    11941 ttacaatttg ctattgtgct ggatttcatg gtgatgtcgc cgctgagcgc tcagttgatg
    12001 cccagcctga agattaatcc gacccagttt ggcctcgtcg tttcggcgta tgcgttcagc
    12061 gcgggagcat cgggattgct ggcggctggt tttgccgaca aatttgaccg caaaaggctg
    12121 cttttgtttt ttatggctgg ttttctcatc ggtaccctgc tgtgcggttt ggcccccaac
    12181 tatccgtttt tgttgggtgc gcgcatcgtc acgggcttgt ttggcggcgt tatcgggtct
    12241 gtcagttttg ccatcatcac cgatttgttt gaggtgcagc agcgtggccg cgtgatggga
    12301 tttgtacaaa tggccttcgc cgtcagccag gtattggggc tgccggtggg cttgtacttg
    12361 tcgaatattt gggactggca cgcgccgttt ttgatgattg tagtggtggc tacgcctttg
    12421 tggttcgtca tcgccatgcg catgaggccc gtcaacgcgc acttggcttt gcaaaaaaag
    12481 cagtcggctg ccgggcacct gttggccact gtatccaaac ccttctatct cagggcattt
    12541 gctgctacca ccttgctggc caccggcggt tttatgctca tgcccttcgg agcggccttt
    12601 gccgtcaata acctcggcat cggcatggac gaccttcctg ttatctacct cgccaccggc
    12661 gttgtttcca tgatcgccgg gccgctgatc ggcaagctca cagatcgcta cagccccatg
    12721 ccgctttttc tggcgggcac cattttgagt atagtgctgg tgcttgttta tacccacctg
    12781 ggaatcacgc cgttgtgggc gctcattgtg ctcaatgctg ttttgtttgt gggaataacg
    12841 gcgcgtatga tttcgtcatc tacgcttgtt acctctgtgc cgacgactgc cgacagaggc
    12901 gcatttatgg gggtcaactc atcggtggcg caaatatcgg gcggtattgc ctctgccacc
    12961 gccgggctga ttatacatca gacggagagc gggatgctgg aaaactaccc cttactcggc
    13021 tgggtggtag ccacttcgat ggtgatctgc gcgctgctgt tttggggcat tactaaagcg
    13081 gtgcggaaac gggacagagc cttggcaaag gggtgagcgt gcagtaaaac gaacgttaag
    13141 atggtacttc gtttgaggca ggttgctatc tttgccagac gaaaagcctt tgcctcgggg
    13201 gcatccgggg actgacatgt tgaatgagat atacccaaaa acctgagaaa acactttgac
    13261 gatgagaaaa tttacacacc tgatatcatt gattttctgt tgtttgtgtt ttgtgtatgt
    13321 ggcacaaggg cagcctgttt ccgtttccaa tccgtccaat tgcggcctgc aaattttact
    13381 ttccgacaat aactgccccg aaaacaaccc caacatttac caacccgatg agataggcat
    13441 tcaggtcgtc ggccaaccag gcaccgcgct cggcattgac gtatatctca aagaagtgcg
    13501 catcattctg gagcatacct ggatgagcga catgaatttg tcgttggttt ctcccggagg
    13561 ccgcgaagtg ttgctcatcg ccaatcaggg cggcagcggc gatcacatag gcaaccccgt
    13621 ggatgtgtcg tgtatgcaat ttgccacgtt cagcgccggc gcttgtacgc ccatttctgc
    13681 aggagcgcct ccatatacag accaagccta tcttcccatt caaagcctgt tgggcttcaa
    13741 cgacagcatc accgcgccca atggtacctg gctcttgcgc atctgcgacg atgtgcccaa
    13801 cgacgtaggc tctttgcagt atgtggagtt ggtttttgag ccggtttcct gcctgcctat
    13861 tcaacaaaaa caggtattga gcgtggactc cacaaccgta gtgctggatt ggtcgcccgt
    13921 tggagattgc aataatttca tcatcgaata tggagcgccg ggatttgtgc cgggtacggg
    13981 cgccgatggc ggagaaggcc aaacggaggc cgtcatcatc tgtccgcctt acccgctgat
    14041 ggacctcctc cctgacacgg agtacgatat ttacatccgc aggttttgcg ttggcggtgg
    14101 attttcgggc aacggctgcc ccatcagagt acgaaccggt tgccaaccgc ctcctaccac
    14161 catcgttgaa acattcgacg cggcgacgcc ttgttttcct aattgtggag cagtgtgcaa
    14221 cataaacggt ttttggcgca atgccggtag cgacgacttc gactggttag tgtatcaagg
    14281 agcaacgccg acgcccaata ccggcccgct tgcaggtttc aacgacgcag gcaggtacgt
    14341 ttacatcgaa acatcggggg tgttatgcac caatggcaag cgcgccattc tggaatccaa
    14401 ttgcgttcgg ctccgcaagt tcaatacaga tacctgccat ctgtcttttg cctatcacat
    14461 gtggggcgtc aacatcaata ccctgcaatt gcaagtgtcg agcgatggcg gcttttcatg
    14521 gaacacgctg tggcaacgca ctggcagtca gggtaatggt tggaaaaaag catacatcgg
    14581 gctggggact tacccggaag gggcggtgct caaattccgc ttcatagcca ccggcggcaa
    14641 cggttcgctg ggcgatattg ctttggatca aatcgtgttt cacggctc
//
LOCUS       DAORLF010000052        14579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2216_length_14579_cov_2.024167, whole genome shotgun sequence.
ACCESSION   DAORLF010000052 DAORLF010000000
VERSION     DAORLF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14579
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_14579_cov_2.024167"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK198_06515"
     CDS             <1..343
                     /locus_tag="PK198_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38420.1"
                     /translation="WILQLMAVLGIGDVDQTDPVLRATARYLVKIGADKQNSFEYAML
                     NHAKKLSQALPAEEKKLLSEWRGYLTELRTGGAGKVSMGLDELALYWVNSRLEKTPII
                     QQIRQQRAGKK"
     gene            524..2290
                     /locus_tag="PK198_06520"
     CDS             524..2290
                     /locus_tag="PK198_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF38421.1"
                     /translation="MTQFLKTAATIAFFALTSLSAFATGTLYVYFDGACMDRMEYSDA
                     AGGKFIAYQINTSTSEKIGLVVGTENPNPEALLPPQTLHCGNTIFDQRFVQAVNQRLS
                     EVYMVRPSGDGRYHITKVAYATHFGNSPDLMVYQSPKAQFSFQTNAGIIGENISLNKA
                     VNAVLFEGRIENACTGEYIFSVTSTSGSGAFSSLVIAPEIGLVEERSGPNIEEAMRNV
                     LRLDKVNDRPVSEYLRIVCRGERPVPVTSGSPVASNQQQLTPRSGDTPVPADNTVPPS
                     YDQPSTQPIAVQPAPASPCAEASGNGFHVVQKGDNLYRISQAYGISVAQLREWNGVSA
                     TNTIYPCQKLRVAAGTAQQAAPPSYSAEQLTPKTVKVLPAWQASTGTHTVQPGETVAA
                     IAMKYGYTEYRFRHFNGLSDNEPARVGQALKTTDCEAQTIGTVTPQQPKQSAYENVLT
                     PRTPAVPAENTGAGKIRSVSTVAASATETELTSSSWKTAASTTATYTPPANMPAPYET
                     SGGRLSSGLSARMQGNETAVPSSYDANSTPAGKRATHLVGKDETLFDIARRYNTTTEN
                     LRRLNNLQPGEAIIPGQRLFVN"
     gene            complement(2356..2928)
                     /locus_tag="PK198_06525"
     CDS             complement(2356..2928)
                     /locus_tag="PK198_06525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRF38422.1"
                     /translation="MTEKEKMLNGQYYRSRDPELLEMYHRARKLLNTYNLLDSKNLQD
                     REHILTELLGSKGLGVWIEAPFFCDYGVNIFIGDHTFINTNCMFLDDNKITIGKNALI
                     APYVQIYTATHPLSASERIVENEAEVYYLTRSEPVTIGDCVWIGGNSVIFPGVTIGNN
                     VTIGAGSVVTKDVPNDVLAYGNPCKVIKHL"
     gene            complement(2945..3697)
                     /locus_tag="PK198_06530"
     CDS             complement(2945..3697)
                     /locus_tag="PK198_06530"
                     /inference="COORDINATES: protein motif:HMM:NF018749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HRF38423.1"
                     /translation="MNLIIADQSDNRHFIMEKSLTILLTSLLIWSCNTRQSDTSADSS
                     ETSISVPPTNFTNDYLERLNWLSQPRSATVKEGILAVTVDKSTDFFNNPEDGAISTTA
                     PLLYEKIEGDFVVKALVRPDFEEVWNAVSLMVYMDSLHWIKFAFENSDATGPSVVTVV
                     TKGVSDDANGAIMSGQKEVWLKLIRKNNNYSMLWSLDDKEYKMARLTSMPAADAVKVG
                     IEAQCPVGESATHEILYYHSERKTVQDLRKGE"
     gene            complement(3694..3873)
                     /locus_tag="PK198_06535"
     CDS             complement(3694..3873)
                     /locus_tag="PK198_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38424.1"
                     /translation="MQKSEHLPKINDEDLKEIKTLLAQYFMDKASDLADEIWEQKGLS
                     EEAILGEHRRTQMRK"
     gene            complement(3980..4180)
                     /gene="rpsU"
                     /locus_tag="PK198_06540"
     CDS             complement(3980..4180)
                     /gene="rpsU"
                     /locus_tag="PK198_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRF38425.1"
                     /translation="MLIVDVKSGDNIDQALKKLKRKFQNAGVLRELRRRREFVKPSVK
                     RRNEIIKAVYIQQKKRDEGEIG"
     gene            complement(4270..5898)
                     /gene="groL"
                     /locus_tag="PK198_06545"
     CDS             complement(4270..5898)
                     /gene="groL"
                     /locus_tag="PK198_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRF38426.1"
                     /translation="MMAKEIHFDLGAREQLKAGIDKLANAVKITLGPKGRNVILQKSF
                     GSPHVTKDGVTVAKDIQLQDPVENLGAQIIREVASKTSDLAGDGTTTATVLAQYMVEH
                     GLKNVTAGANPMDLKRGMDKAVKALVAHIKSQSEDISHDFDRIRQVATISANNDRSIG
                     DMIADAMQRVTKDGVITVEEAKGMETYVEEVSGMQFDRGYLSPYFITNTERLITEYDS
                     PLILLTEGKISNIREFLPILEKAASTGRALLVIAEDVDSQPLGVLVVNRLRGSLRIVA
                     VKAPGFGDRRKAMLEDIAALTGATVISEEKGYKLENTELEHLGSAEKIIVDKDNTTIV
                     NGSGDPEMVKARIKQIKQQIESTTSSYDKEKLQERLAKLSGGVAVLHVGAATETEMKE
                     KKDRVDDALHATRAALEEGIVTGGGVALIRAGEALNDLKGLNEDETTGIEIIRKAIEY
                     PLRIIVTNAGMEGSVVVHKVREGQGAFGFNALTDVYEDLKAAGVVDPAKVVRVALENA
                     SSIAGMVLTTEVVIHEKPKKEAASGHDHGMEDYG"
     gene            complement(6014..6481)
                     /gene="smpB"
                     /locus_tag="PK198_06550"
     CDS             complement(6014..6481)
                     /gene="smpB"
                     /locus_tag="PK198_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRF38427.1"
                     /translation="MAEKKPKTIEIVNRRARYEYEFLDVFEAGIVLQGTEIKSIRKGN
                     ANLSDAYCCFQPDGLYVRSMFIAEYENGTYANHETRRVRKLLLRKPELRKLERRVKER
                     GLTIVPYKLYLSDRGLAKLEIALAQGKKTHDKRESIKEKDNKRELDRMKKIPK"
     gene            6677..>6952
                     /locus_tag="PK198_06555"
     CDS             6677..>6952
                     /locus_tag="PK198_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRF38428.1"
                     /translation="MLESESRQALLLLADGTLFRGKAAGAVGTTTGEICFNTAMTGYQ
                     EIFTDPSYFGQLMVTTNAHIGNYGIKATETESATIKIAGLICKNFSEP"
     assembly_gap    6953..6976
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6977..7778
                     /gene="carA"
                     /locus_tag="PK198_06560"
     CDS             <6977..7778
                     /gene="carA"
                     /locus_tag="PK198_06560"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRF38429.1"
                     /translation="IQKYFEAQGLVAISDVDTRAIVRHIRSKGAMNAIVSSEITDVEE
                     LRRRLAAVPNMDGLELASAVSTPEPYFVGNPDARFRVAVYDYGVKRNILQCFAERDCY
                     LQVFPAKTPLGQVKDWKPNGYFLSNGPGDPAPMDYAVQTAKSILAEDLPLFGICLGHQ
                     ILALAMDIPTHKMHHGHRGINHPVKNLITGRSEITSQNHGFGVSKDAIAAAADRVEVT
                     HVNLNDDTIEGMRVKGKRAFSVQYHPEASPGPHDARYLFDDFIRFMQI"
     assembly_gap    7949..8112
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8113..9128
                     /gene="eno"
                     /locus_tag="PK198_06565"
     CDS             <8113..9128
                     /gene="eno"
                     /locus_tag="PK198_06565"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRF38430.1"
                     /translation="IDNTMLDLDGTPNKAKLGANAILGVSLAVAKAAAEEAGMSLYRY
                     VGGVNAHVLPVPMMNILNGGSHADNGIDFQEFMIMPIGAESFSQGLRMGVEVFHNLKS
                     VLKGKGYSTNVGDEGGFAPNIKSNEEAIQIVLQAIEAAGYRPGEDIMIALDPAASEFY
                     NEEEQVYHLRKSSGEKLTGDQMVAYWKDWCSRYPIFSLEDGLAEDDWASWGKLTAEIG
                     SRVQLVGDDLFVTNTKRLQRGIEANVANSILVKVNQIGSLTETIETVSMATRNAYTSV
                     MSHRSGETEDTTIADLAVGLNTGQIKTGSASRSDRIAKYNQLLRIEEELGDDAVYPGK
                     SLL"
     gene            9159..9464
                     /locus_tag="PK198_06570"
     CDS             9159..9464
                     /locus_tag="PK198_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38431.1"
                     /translation="MFSNLFKPLTDLLPHQLRNKYFLILAVFFGLLIFVDKHDLITQY
                     KLQRTVNKLEQDKAFYREKIDEAEANRASFNENKERYAREHYKMKRADEDVFIIVEE"
     gene            9615..10487
                     /gene="sucD"
                     /locus_tag="PK198_06575"
     CDS             9615..10487
                     /gene="sucD"
                     /locus_tag="PK198_06575"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRF38432.1"
                     /translation="MSVLVNKNSKIIVQGFTGKEGTFHASQMIEYGTNVVGGVTPGKG
                     GETHLDKPVFNSVYDAVQQAGADTSVIFVPPAFAADAIMEAAEAGIKVIICITEGIPV
                     QDMVKVKAYISGKDCRLIGPNCPGVITPDEAKCGIMPGFIHRKGNIGIVSRSGTLTYE
                     AVDQVTKAGMGQSTCIGIGGDPIVGTTTKEAVELLMNDPETEGIIMIGEIGGSMEADA
                     ARYIRDFGTKPVVGFIAGQTAPKGRTMGHAGAIIGGADDTAEAKMKIMEECGIHVVRT
                     PAEIGETMRRALGK"
     gene            10772..11497
                     /locus_tag="PK198_06580"
     CDS             10772..11497
                     /locus_tag="PK198_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF38433.1"
                     /translation="MPLNNTTPPGALYLIPAPLGESGLDSIPAAAIAVLHRLEYFIVE
                     RAKTARHYIKATHPPRALQELHIVELAQNSATEDWEALLAPLRAGHDVGLLSEAGCPG
                     IADPGAEVVKRAHLHGIQVVPLAGPSSIVLALMASGMNGQSFCFHGYLSAKRPELERD
                     LKRLEQSALRLHQTQLFIEAPYRNGQVIETALKTLSAGTLFGIACDLTLPEQYVRRLP
                     VSEWRETPPPDLHKRPAVFLIGS"
     gene            11509..12141
                     /locus_tag="PK198_06585"
     CDS             11509..12141
                     /locus_tag="PK198_06585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF38434.1"
                     /translation="MQSFWNERYAAPEYAYGKAPNVFLASRLPDFPAGRILFPAEGEG
                     RNAVFAARLGWEAHAFDYSESARMKALQLAQEQGVQLQYAVADVRELDYEPAAFDAIA
                     IIFAHFPSSFRAHCHQVWDRLLKPGGVLLLEAFSKDHLSYNADGKAGGPREADLLYDA
                     AELEAAFGHYDILESRQVETELSEGLYHNGLSSVVQFVARKPVNGPAFGQ"
     gene            complement(12116..12556)
                     /locus_tag="PK198_06590"
     CDS             complement(12116..12556)
                     /locus_tag="PK198_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRF38435.1"
                     /translation="MHPPTEETEEPEYFKDRRLMISVFMSPVNVHVNRSPIPGQVRYS
                     QYHPGKYLVAWHPKSSTENERHTSVIGNEHGDILLRQIAGALARRICNYLTPGQTIEQ
                     GAEMGFIKFGSRVDIFLPLKTEIKVKIGDVVRANRSVIAQMPGR"
     gene            complement(12513..12755)
                     /locus_tag="PK198_06595"
     CDS             complement(12513..12755)
                     /locus_tag="PK198_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38436.1"
                     /translation="MKIHKEGYASLAVVFAVGLALSGLSYWMIPSLFGVAIIVSILLF
                     IIVLQFFRHPERSICEADKCCVYAPADGRNGGTGIF"
     gene            12839..13924
                     /locus_tag="PK198_06600"
     CDS             12839..13924
                     /locus_tag="PK198_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38437.1"
                     /translation="MMKRIYMAATSQHVGKTTCTLGLTAALRRRGLNVGYCKPVGQEF
                     VDLGDLKVDKDALLFSTIMGFILDPALHSPVIVGSGLTKSFIENPGEYQFAERVLHAA
                     NSLDSVHDIVVMEGTGHPGVGSVIDLSNADVAALTGAGVVMVVEGGIGSTIDELNVNL
                     ALFREQKLPILGVIVNKVLPDKMEQVRHYVGKKLDRYGIPLLGVLPFDKTLTFPIMET
                     ILQAVKGKVVFHEERLDNRIEHIISGSLVERREFGGFHNLCRLDHTIEIIEEVARQKG
                     MEGSPLAGIIVNGEGDFVPEFLDDFTCKDYVEAHRIPVIATTLDTYGAALKIGKIEVK
                     INTRTLWKAERAVELFQQHVELDRLLG"
     gene            complement(13971..>14209)
                     /locus_tag="PK198_06605"
     CDS             complement(13971..>14209)
                     /locus_tag="PK198_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38438.1"
                     /translation="LRRDDTFRSNCFIKMLLVIPAQGFHKSAVLRHAEKYHKMLLSMP
                     IDMANQSHDVEIIPYEDLWEMALESLQNKTHKKK"
     assembly_gap    14210..14219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14220..>14579)
                     /locus_tag="PK198_06610"
     CDS             complement(<14220..>14579)
                     /locus_tag="PK198_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38439.1"
                     /translation="YQELLIFLSLHTRQYQESYKIFKQTTGHRRFGALPRAIGETWRI
                     MEAYLFYLVELGKIIPAEDDTVFSKFRMARFINEMPLFSRDKRGMNIPILIFQILSLI
                     YNKRYDAAIDRMEAIEKY"
BASE COUNT         3270 a         3918 c         3784 g         3409 t
ORIGIN      
        1 ctggattttg cagctcatgg cggttttggg catcggcgat gtggatcaga cagaccccgt
       61 gttgcgcgcc accgcacgct accttgtcaa aataggggcg gataagcaaa atagctttga
      121 atacgccatg ctcaaccacg ccaaaaaatt gtcgcaggcg cttcccgctg aggagaaaaa
      181 attgctctcg gaatggcgcg ggtacctcac cgagcttcgc accggcggcg caggcaaggt
      241 ctctatgggt ttggacgaac tcgcccttta ctgggtcaac agtcgcttgg aaaaaacgcc
      301 cattatacag caaatcaggc agcaacgcgc gggaaagaag taagacctcg cccgactttt
      361 aagtgcctcc ggagttcgct ttttcgtaat ttttttgttg gaaatacgtt tgttgcaaaa
      421 caaatgcaat tgattttcag ttatttgcga aaaacagcca aaaaacaact tgcgtaaaat
      481 gctgattatt cgtaccttac gctttcaaat caacaataaa accatgacac aattcctaaa
      541 aactgctgca acgatcgctt tttttgcgct gacttccctg tcggccttcg ctaccggcac
      601 gctgtacgtt tacttcgacg gggcctgcat ggaccgcatg gaatactccg acgccgccgg
      661 tgggaaattc attgcatacc agatcaacac ctccacctct gaaaaaatcg gcttggtggt
      721 cggcaccgaa aaccccaatc cggaggctct gctgccgccc cagaccctgc attgcggcaa
      781 caccattttt gatcagcgct ttgtgcaggc cgtcaatcag cgcctcagtg aagtgtatat
      841 ggtgcgccct tccggcgacg gccgctatca cattaccaaa gtggcttacg ctacgcattt
      901 cggcaactcg cccgatctga tggtctatca gtcgcccaaa gcgcaatttt cctttcaaac
      961 caatgccggc atcatcggtg aaaacatttc gctgaacaaa gccgtcaacg ccgtgctctt
     1021 tgaaggccgc atcgaaaatg cctgcaccgg cgaatatatt ttcagcgtaa cgagtacttc
     1081 tggctcaggt gctttcagca gcctcgtcat cgctcctgaa ataggtctgg tggaagaacg
     1141 cagcggtccc aatatcgaag aggccatgcg caatgtactg cgtttggata aagtaaacga
     1201 ccgcccggtg tcggaatacc tgcgcatcgt atgccgcggt gagcgcccgg taccggtgac
     1261 gagcggatcg cctgtcgctt ccaaccaaca acaactgacg ccccgcagcg gcgacacgcc
     1321 cgtacccgcc gacaataccg tgccgccttc ctacgatcag ccttccacac agccaattgc
     1381 cgttcagccc gcacctgctt cgccctgcgc agaggcctcc ggaaatggct tccacgttgt
     1441 acaaaaaggc gacaacctct accgcatttc tcaggcctac ggcatcagcg tggctcaact
     1501 gcgcgagtgg aacggcgtga gcgctaccaa taccatttat ccttgtcaga aattgcgggt
     1561 ggcagcaggt actgctcaac aggctgcgcc gccgagttat tctgcggagc aactcacgcc
     1621 caaaaccgtc aaagtcttgc cggcatggca ggcatctacc ggcacgcaca cggtgcaacc
     1681 cggcgaaacc gtcgcggcca ttgccatgaa atacggctac acggagtatc gcttccggca
     1741 tttcaatggt ttgagcgaca acgagccggc cagagtgggt caggcgctca aaacaacaga
     1801 ctgtgaagcg caaaccatcg gcactgtaac tccgcagcaa cccaaacaat cggcttatga
     1861 aaacgtgctg acgccccgaa ctcctgccgt gcctgccgaa aataccggcg caggcaaaat
     1921 tcgctcggtg agtaccgtcg ccgcttctgc cacggaaaca gaactgacct cctcctcctg
     1981 gaaaacggct gcttccacaa cggctacata cactccgccg gccaacatgc ccgcgcccta
     2041 cgaaacttca ggaggccgcc tcagttcagg actttccgct cgcatgcaag gcaatgaaac
     2101 cgccgtgccg agcagctacg atgccaacag cacgcccgcc ggaaaacgcg ctacgcacct
     2161 ggtaggcaaa gacgaaacct tgttcgacat tgcccgccgc tacaatacga cgacagagaa
     2221 tctgcgccgc ctcaacaacc tccaaccggg tgaggcgatc attccggggc agcgtttgtt
     2281 tgtgaattga gcagcgccgc gaggctccat ttcgtgtcaa tgagcgaagt ggagcctccg
     2341 gtttcgccgg cagtgctaaa ggtgcttgat aactttacat ggatttccgt aagccagcac
     2401 gtcgtttgga acatctttgg tgacgacgct tcctgcacct attgttacat tgtttcctat
     2461 ggtcactccg ggaaatatga ctgaattccc tcctatccaa acacaatctc cgatggttac
     2521 aggttctgag cgcgtcaggt aatacacttc ggcctcgttt tcaacaattc tttcggaagc
     2581 actcaatggg tgtgttgctg tatagatttg tacatacggc gctatcagcg cattttttcc
     2641 tatggtgatt ttgttgtcgt ccaaaaacat acagttggtg ttgataaaag tatgatcgcc
     2701 aatgaaaata ttgacgccat aatcgcagaa aaagggcgcc tcaatccata cgcccaggcc
     2761 cttgctgccc aatagttctg taagaatatg ctctctgtct tgtaggtttt tggagtccag
     2821 tagattatat gtgttcaata gttttctggc tctgtggtac atttccaaca gttcggggtc
     2881 tctcgaacgg tagtattgac cattcaacat tttctccttc tctgtcatgt gcgaggattt
     2941 ttactcattc accttttctc aaatcctgca cggttttcct ctctgagtgg tagtatagaa
     3001 tttcatgagt ggcagattct ccaaccgggc attgtgcttc tatcccaact tttacagcat
     3061 ccgcagcagg catggaggtt aatcgtgcca ttttgtattc tttatcatcc agagaccaaa
     3121 gcatggagta attgttgttt ttgcgaatca gtttgagcca aacctctttt tgcccgctca
     3181 tgatggcgcc gttggcatcg tccgataccc cttttgttac aacagtgacg acgctggggc
     3241 cggtagcatc cgagttttca aatgcaaact ttatccaatg gaggctgtcc atgtaaacca
     3301 tcaacgacac ggcgttccac acctcttcaa aatcaggtct taccaacgct ttgaccacaa
     3361 aatcaccttc aatcttttcg tacaacaatg gagctgttgt ggatatggcg ccgtcttccg
     3421 gattgttgaa aaagtcggtg cttttatcaa cggtaacagc cagaatacct tctttgacag
     3481 ttgccgaccg aggttgggac aaccagttca gccgttctaa ataatcattg gtaaaattgg
     3541 tgggaggcac acttatactc gtttcggagg aatccgccga agtgtctgat tgtctggtat
     3601 tgcaactcca aatcagcagc gaagtcaaaa gaatggtcaa tgatttttcc atgataaagt
     3661 ggcggttatc tgattgatca gcaatgatta ggttcattta cgcatctgag tacggcggtg
     3721 ttcgccgaga atcgcctcct cgctgagacc tttttgttcc cagatttcat cggccaggtc
     3781 ggaagccttg tccatgaaat attgagcgag taatgtttta atctctttca agtcttcgtc
     3841 attgattttc ggcagatgct ctgatttttg catggaggct gaatgtgttt tctacaatac
     3901 tcgaaacaac aaaggcttgg cgcaatcctg atagatgcgc caagcctgat aaaaacaaag
     3961 tatgatgtaa gcgaacggat tatccgattt cgccttcgtc gcgttttttc tgctggatat
     4021 aaacggcctt gatgatttcg ttgcggcgct tcacagaagg cttcacgaat tcacggcgac
     4081 ggcgcagttc gcgcagaaca cctgcatttt gaaattttct tttcagtttc ttcagcgcct
     4141 gatcaatgtt gtcaccgctt tttacatcaa caataagcat aaagtttctc cttttttgtt
     4201 tcggactgca aaggtaaagc gtcagatcaa atttccaacg ccttaccgtt tattttttga
     4261 tattttttct cagccgtagt cttccatgcc gtgatcgtgg ccgctggcgg cttctttctt
     4321 cggcttttcg tggatgacga cctccgtggt gagcaccatg cctgcaatag aggacgcgtt
     4381 ttcgagggct acgcgcacta ccttggcggg gtccactacg ccggcagcct tgaggtcttc
     4441 atacacatcg gtgagcgcat tgaacccgaa agcaccctgg ccttcgcgta ctttatgcac
     4501 caccacagag ccttccatgc cggcattggt aacgatgatg cgcagcggat actcaatggc
     4561 tttgcgaatg atctcgatgc ctgtggtttc gtcctcgttg agacctttga gatcgttgag
     4621 ggcttcacct gcgcgaatga gggctacgcc gccgccggtg acgatgcctt cttcgagggc
     4681 agcgcgggtg gcatgcagcg cgtcgtccac gcggtctttt ttctccttca tttcggtttc
     4741 ggtagcagcg ccgacatgca gcacggccac gccgccggag agtttggcca agcgttcctg
     4801 caatttttcc ttgtcgtagg acgaagtggt gctttcaatt tgctgtttga tctgcttgat
     4861 gcgggctttc accatttcgg gatcgccgct gccgttcacg atggtggtgt tgtctttgtc
     4921 cacgatgatt ttttcagcgc tgccgaggtg ctccaactct gtattttcga gtttgtaacc
     4981 tttttcttcg ctgatgacgg tggcgccggt gagggctgcg atgtcttcga gcatggcttt
     5041 gcggcgatct ccgaagcccg gggctttaac ggccacaatg cggagcgaac cgcgcaaacg
     5101 gttgactacc aatacgccca gcggctgact gtccacgtct tcggcgatga ccaacaaggc
     5161 ccggccggta gaggctgctt tttccaaaat gggcaggaac tcgcggatgt tggaaatttt
     5221 gccctcggtg agcaaaatga gcgggctgtc gtactcggtg atgaggcgct cggtattggt
     5281 aataaaatat ggagagaggt agccgcggtc gaactgcatc ccgctcactt cttccacata
     5341 cgtttccatg cctttggcct cctctacggt gatgacgccg tctttggtca cgcgctgcat
     5401 ggcgtcggca atcatgtcgc cgatgctgcg gtcgttgttg gccgagatgg tggctacctg
     5461 ccggatgcgg tcgaagtcgt ggctgatgtc ttccgactgc gatttgatgt gtgccacgag
     5521 ggctttgacg gctttatcca tgccgcgctt gaggtccatc ggattggcgc cggcggtcac
     5581 gtttttgagg ccgtgttcta ccatgtattg agccagtacg gtggctgtgg tggtgccgtc
     5641 gccggcaagg tcggaagttt tggaagccac ttcgcggatg atctgcgcgc cgaggttttc
     5701 caccgggtct tgcagttgga tatccttggc tacggtgacg ccgtctttgg tgacgtgcgg
     5761 cgatccgaag cttttttgga ggatgacgtt gcggcctttc ggtcccaggg tgattttgac
     5821 ggcgtttgcc aatttgtcaa tacccgcctt gagttgttcg cgggcgccga ggtcgaaatg
     5881 gatttctttt gccataatat gcagatgaat ggttggttaa acgaatcgct ctccaacggg
     5941 caatctgcgt gccaattcat tgatacggca aaaccggggt gtcgttgtgt caatttggca
     6001 gggtagggga gtgttatttg ggtattttct tcatgcggtc caattctcgc ttgttgtctt
     6061 tttctttgat ggattcgcgt ttgtcgtggg tctttttgcc ttgtgccagc gcgatttcga
     6121 gcttggccaa gccgcgatct gagagataga gcttgtaggg cacaatggtg aggccgcgct
     6181 ctttgacgcg ccgttccaat ttgcgcagtt cgggttttcg cagcagcagc ttgcgaaccc
     6241 ggcgggtttc gtgattggca taagtgccgt tttcgtactc ggcaatgaac atgctgcgca
     6301 catacaggcc gtcgggttga aaacagcagt aagcatccga gaggttggcg ttgcccttgc
     6361 gaatggattt gatctcggtg ccctgcaata cgatgcccgc ctcgaatacg tcgaggaatt
     6421 catattcgta acgcgccctg cgattcacga tttctatggt ttttggcttc ttctctgcca
     6481 tgacgttgtt tgatttctga ttccgggttc caaattgcac tgcctactgc ccactacaca
     6541 ccgcctaccg ctccgtcttt acaagccccc tccgaacatc gcacagcaaa attaagtttc
     6601 gtttgcggat tttattcccc taatgactgc ttttcgctat ctttgccgcg tcaattttga
     6661 aacacaccgc acgtccatgc tggaatcaga atcaaggcag gccctgttgt tgttggcaga
     6721 cggcacgctc tttcggggca aagccgccgg cgctgtcggc accaccacag gcgaaatctg
     6781 cttcaatacc gcgatgaccg gctatcagga aatttttacc gacccctcct acttcggcca
     6841 gttgatggtc acgaccaatg cccacatcgg caattacggc atcaaagcaa cggaaacgga
     6901 gtcggcaacg attaaaattg ccgggctgat ctgcaaaaat ttcagcgagc cgnnnnnnnn
     6961 nnnnnnnnnn nnnnnncatc cagaagtatt ttgaagcgca gggtttggtg gccatttccg
     7021 acgtggatac ccgcgccatc gtgcggcata ttcgcagcaa aggcgccatg aatgccatcg
     7081 tttcctccga aatcaccgat gtggaggaac tgcgccgccg cttggccgcc gtgccgaata
     7141 tggacggact ggaattggcc tctgccgtga gtacgcccga accgtatttc gtgggcaacc
     7201 ccgatgcgcg tttccgggtg gctgtatatg attatggcgt caagcgcaac attttgcaat
     7261 gcttcgccga gcgcgactgc tacctgcaag tgtttcccgc caagacgccg ttgggtcagg
     7321 taaaggattg gaaaccaaat ggttattttc tgtccaatgg tccgggcgac cccgctccga
     7381 tggattacgc cgtgcaaact gccaaatcca tcctggccga agacctgccc ttgtttggta
     7441 tatgtctggg gcatcagata ctggcgctgg ctatggacat tcccacgcac aaaatgcacc
     7501 acggccaccg gggcatcaat catccggtga aaaatctgat taccggcagg agtgaaatca
     7561 ccagccaaaa tcatggtttc ggcgttagca aagatgctat tgccgctgcc gccgaccgcg
     7621 tggaggtaac gcacgtcaac ctgaacgacg acaccatcga aggcatgcgc gtgaagggca
     7681 aacgagcttt ttcggtgcaa taccaccccg aagcctcgcc cggcccgcac gacgcccgct
     7741 acctgttcga tgattttatc cgtttcatgc agatttaata tggccgcagg ggctttcctt
     7801 ctatttttgc caccacaatc attagccaac tgaaaaaata aataacatga gtgcaattgc
     7861 agacatcatc gcccgcgaaa tcctcgactc ccgcggcaac cccaccatcg aagtggaagt
     7921 attgaccgag gacggtttct ttggccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnacattgac aataccatgc tcgacctcga cggcaccccg aataaagcca
     8161 aactcggcgc caatgccatc ctcggcgttt cattggccgt agccaaagcc gccgccgaag
     8221 aggccggcat gtcgctgtat cgctatgtgg gcggcgtcaa tgcgcacgtt ttgcccgtac
     8281 cgatgatgaa catcctcaat ggaggttctc atgccgataa tggtattgac tttcaggagt
     8341 ttatgattat gcccatcggc gccgagagct tctcgcaggg cctgcgcatg ggcgtggagg
     8401 tgtttcataa cctcaagagc gtgctgaaag gcaaaggcta ctctaccaac gtaggcgacg
     8461 agggaggatt tgctcccaac atcaaatcga acgaagaggc catccagatc gtgctccagg
     8521 ctattgaagc agcaggctac cgtccgggcg aggacatcat gatcgcgctg gaccccgccg
     8581 cttccgagtt ttacaacgaa gaagaacagg tgtatcacct ccgcaagtcc agcggcgaaa
     8641 aactcaccgg cgaccaaatg gtggcttatt ggaaagactg gtgcagccgc tacccgatct
     8701 tctcgctcga agacggcctc gccgaagacg actgggcttc ctggggcaaa ctgaccgccg
     8761 aaatcggcag tcgcgtgcag ctcgtaggcg acgacctctt tgtgaccaac accaagcgcc
     8821 tgcagcgcgg catcgaagcc aatgtggcca actccatcct cgtgaaagtc aaccagatcg
     8881 gctcgctcac cgaaaccatc gaaaccgtga gcatggctac ccgcaacgct tacaccagcg
     8941 tgatgagcca ccgttcgggc gaaaccgaag acaccaccat cgccgatttg gctgtgggtc
     9001 tcaacaccgg ccagatcaaa accggctcgg cttcccgctc agatcggatt gccaagtaca
     9061 accagttgct ccgcatagag gaagaattgg gtgacgacgc ggtgtatccg ggcaaatctt
     9121 tattgtaatt cgtatgtttg tcaaataaaa cgctgcttat gttttccaat ctgttcaaac
     9181 cgctcaccga cttgctgccg catcagcttc gcaacaagta cttcctgatc ctcgccgtct
     9241 ttttcggcct gctcattttc gtggacaaac acgacctgat cacgcagtat aagctacagc
     9301 gcacggtgaa caaactcgaa caggacaaag cgttttatcg cgaaaaaatt gacgaggcgg
     9361 aggccaaccg ggcttcgttc aacgaaaata aggagcgcta cgcccgcgaa cactacaaaa
     9421 tgaaacgcgc cgacgaggat gtgttcatca ttgtagagga atagaatagg gcgtgccggc
     9481 ccctcaagcg cctccccgac cctccaaagg agggagccat cgggcagggc cgtcgggtgt
     9541 tccggtcgtc gcctcccttc accccctcac gcggcagacc aacgacaacg aaaaactgct
     9601 ttaaaataac aaccatgtct gtattagtca ataaaaattc aaaaatcatc gtccagggat
     9661 tcaccggcaa agaaggtact ttccacgcat cccaaatgat tgaatacggt accaacgtag
     9721 taggcggcgt cacgcccggc aaaggcggcg aaacccactt ggacaagccg gtattcaact
     9781 ccgtttacga tgccgttcag caggccggcg ccgacacatc ggtcatcttc gtgccgccgg
     9841 cattcgctgc cgacgccatc atggaggccg ccgaggcagg catcaaagtc atcatctgca
     9901 tcaccgaggg catccccgtg caggacatgg tgaaggtgaa agcctacatc tccggcaaag
     9961 actgccgcct catcggaccc aactgccccg gcgtcatcac gcccgacgag gccaaatgcg
    10021 gcatcatgcc gggcttcatt caccgcaagg gcaacatcgg catcgtttcg cgttccggca
    10081 ccctcactta cgaggcagtg gaccaagtga cgaaagccgg catgggccag tccacctgca
    10141 tcggcatcgg cggcgacccc atcgtgggca ccaccaccaa agaagcggtg gaactgctga
    10201 tgaatgaccc cgaaacggag ggcatcatca tgatcggcga gatcggcggc agcatggaag
    10261 ccgacgccgc ccgctacatc cgcgacttcg gcaccaaacc ggtggtaggt tttattgccg
    10321 gccaaacggc gcccaaaggc cgcaccatgg gccacgccgg cgccatcatc ggcggcgcag
    10381 acgacaccgc cgaagctaaa atgaagatca tggaggagtg tggcatccat gtggtgcgta
    10441 ctcccgctga aatcggcgaa accatgcgcc gggctttggg gaaatgagtt tttaggtttt
    10501 gacagtgcgg gtagaacccg ccgaatattg gcacgagatg gaatctcgcg ccagcgaagg
    10561 aagggcgttg ccggagggtg gcgtcctttt ttcgttgtgc cgcgatgggc aaaaagagca
    10621 aaaatagccc gcgcgaggtt ctacctcgtg tgaactatcg tgcgggttcc acccgcttgt
    10681 aatcagctca atttcagtat gaacctcgtg ccggcgtagg aaaacacatc cctcgcgcaa
    10741 aaaatccatc tcacccgcct atctttgcgc catgccgctg aacaacacca cgccgcccgg
    10801 cgccctctat ctcatccccg cgccgctggg cgagtccggg ctggattcca tacccgcagc
    10861 ggccatagcc gtgttgcaca ggttggaata cttcattgtg gagcgggcca aaacagcgcg
    10921 ccactacatc aaagccaccc atccgccgcg cgccttgcag gaattgcaca tcgtagaatt
    10981 agcgcaaaac tccgccaccg aagactggga agcattgctg gcgcccctgc gcgcaggcca
    11041 cgatgtgggc ttgttgtcgg aagcgggctg ccccggcatc gccgaccctg gagcggaggt
    11101 ggtaaaacgg gcgcacttgc acggcataca ggtggtgccc ttggcggggc cttcgagcat
    11161 cgtactggcg ttgatggcat cgggtatgaa cgggcagtcg ttttgctttc acggctacct
    11221 gtcggccaag cgcccggagc tggaacgcga cctcaaaaga ttggagcaga gcgccttgcg
    11281 cttgcatcaa acccaactgt tcatagaagc gccctaccgc aacgggcagg tcattgaaac
    11341 agccttgaaa accctgagcg cgggaacctt attcggcatt gcctgcgatc tgacgctgcc
    11401 ggaacaatat gtgcgtcgcc tgccggtgtc ggaatggcgc gaaacgccgc cgcccgattt
    11461 gcacaaacgt cctgcggtgt ttttgatagg ttcgtaaaat accacatcat gcaatcattc
    11521 tggaacgagc gctacgcagc gcccgagtac gcctacggaa aggcgcccaa tgtttttctc
    11581 gcttcccggc tgcctgattt tccggcgggc cgcatccttt ttcccgccga gggagaaggg
    11641 cgcaacgccg tttttgcggc gcgattgggt tgggaggcgc acgcttttga ttacagtgaa
    11701 tctgcccgga tgaaggcatt gcagttggcc caggaacaag gggtgcagtt gcaatacgcc
    11761 gttgctgatg tgcgggagtt ggattacgaa ccggctgctt ttgatgccat cgccatcatt
    11821 tttgcacatt tcccctcttc attccgcgcg cattgccatc aggtatggga ccggctgctg
    11881 aagcccggcg gcgtgttgtt gctggaggcg ttcagcaaag accatttgag ctacaacgca
    11941 gacggaaaag caggcggccc gcgcgaggct gatttgctgt acgatgcggc agaattggaa
    12001 gcagcctttg ggcattatga tatactggaa tcccgacaag tggaaaccga gctttccgaa
    12061 ggattgtacc acaatggctt gtcttctgtg gtgcagtttg tggcacgcaa accggttaac
    12121 ggcccggcat ttgggcaatg acgctgcgat tggccctcac cacgtctccg attttgacct
    12181 tgatttcggt tttcaacggc aggaaaatgt ccacccgcga gccgaactta atgaagccca
    12241 tttcagcgcc ctgttcgatg gtttggccgg gcgtgaggta gttgcatatc cgccgggcca
    12301 atgcgccggc tatttgtcgc agcaaaatat cgccatgttc attgccgatg accgaagtgt
    12361 ggcgttcgtt ctcggtggag gatttgggat gccaggccac caaatatttg ccgggatgat
    12421 attggctgta tcgcacctgt ccgggtatag ggctgcggtt gacgtgcaca ttgacgggcg
    12481 acatgaagac ggagatcatg agccggcgat ctttaaaata ttccggttcc tccgtttctt
    12541 ccgtcggcgg gtgcataaac gcaacatttg tcggcttcac aaatactgcg ttcaggatgt
    12601 ctgaagaatt gtaaaacaat gataaacagg agtatagaga caataattgc aactccgaac
    12661 agcgagggaa tcatccagta cgacagcccc gacaacgcca atcccacagc aaaaacaaca
    12721 gccaacgagg cataaccttc cttatgtatt ttcatcaaat ctgcattttt gccgcaaatt
    12781 tcgtttatca ttcccgaaat gcctaatttt agcgcttctc tcagcacaat gctgcactat
    12841 gatgaaacgc atttatatgg ccgccaccag tcagcatgta ggaaaaacca cctgcacgct
    12901 ggggcttacg gctgcgctgc gccgccgggg cctgaacgtg ggctactgca aaccggtagg
    12961 ccaggagttt gttgacctcg gcgacctgaa ggtggacaaa gacgccttgc tgttttccac
    13021 cattatgggt tttattctcg atccggccct gcacagtccg gtcatcgtgg gcagcggtct
    13081 caccaagtct tttatagaaa accccggcga gtaccaattt gccgagcgcg tcctgcatgc
    13141 cgccaacagc ttagattctg tacacgatat cgtagtcatg gagggcaccg gccatccggg
    13201 agtcggcagc gttattgacc tgagcaatgc cgacgtggct gcgctcaccg gcgccggtgt
    13261 ggtgatggta gtagagggcg gcataggcag caccattgac gagctgaatg tcaatctggc
    13321 tttgtttcgg gagcaaaaac tgcccattct cggcgtcatc gtcaataaag tattgcccga
    13381 caaaatggag caggtgcggc actatgtggg taaaaaatta gaccgttacg gcatccctct
    13441 gcttggcgtg ttgccctttg ataaaaccct tacctttccc atcatggaaa ccattttgca
    13501 agccgtcaaa ggcaaggtgg tttttcacga agagcgcttg gacaaccgca tagagcacat
    13561 catttccggg tctttggtag aacgccggga gtttggcggg tttcacaacc tctgccgcct
    13621 cgatcacacc atagaaatca tagaagaagt ggcccggcaa aagggcatgg aaggtagtcc
    13681 gctggcgggt attattgtca acggagaggg cgattttgta cccgaatttt tagacgactt
    13741 tacctgtaaa gattatgtgg aggcgcatcg cattcccgtc attgccacca cattggatac
    13801 ttatggggcg gcgctcaaga ttggaaaaat tgaggtcaag atcaataccc gcaccttgtg
    13861 gaaagctgag cgggcggtgg agttgtttca gcaacatgtg gaattggaca ggctgctggg
    13921 ctgaggagga tgcgcgagcc aagcccattg agcgcgaacc aaagccggca tcactttttc
    13981 ttgtgcgttt tattttgcag agactccagg gccatttccc agaggtcttc gtaggggatg
    14041 atttctacat cgtgcgattg gttggccata tctataggca tagagagcag cattttgtgg
    14101 tacttttcgg catggcgcag cacagcggat ttgtggaagc cctgcgccgg aatgacgagc
    14161 agcattttga tgaaacagtt gctgcggaaa gtatcgtctc gccttaaatn nnnnnnnnng
    14221 tatttttcga tggcctccat gcggtcaatg gcagcatcat agcgcttgtt gtagatcaga
    14281 gataaaattt ggaagatcaa aatggggatg ttcatgccac gcttgtctct tgaaaaaaga
    14341 ggcatttcat tgataaaacg ggccattctg aactttgaaa aaacagtatc atcctctgcc
    14401 gggatgattt ttcccaactc gactaagtaa aacagatatg cctccatgat gcgccaggtc
    14461 tctccgatcg ctctgggaag ggcgccgaag cggcggtggc cggtagtctg cttgaaaatc
    14521 ttataagatt cctgatattg acgggtatgc aagctgagaa aaatgagcaa ctcctgata
//
LOCUS       DAORLF010000053        14071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2312_length_14071_cov_1.840825, whole genome shotgun sequence.
ACCESSION   DAORLF010000053 DAORLF010000000
VERSION     DAORLF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_14071_cov_1.840825"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PK198_06615"
     CDS             complement(<1..>1217)
                     /locus_tag="PK198_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38440.1"
                     /translation="ANTGSAGFFVFADGAISGPHSYSNAGVTVGPFPGNGTNIVDILI
                     LDISNPACYGYIEVGPLNCDDLDCHITELTAVASDCNDADEFFVELDFVPTGNHSDGF
                     TVRGNGVFYGTFDYDDLPIRLGPFGLNTPPVLEFSVRDQESPETCRAVITVQRPVCNP
                     IIVWPGDTDNNNIANHFDLLNIGLAFGTQGPPRSANSADWSGMAATPWQQMFNTNDLN
                     FAYADCNGNGIVNTGDVEVLIENYGLTHGPVAPYTPLPATPNNPRIIVDMPAGPIAPG
                     TQISAPILLGGSVVSVEAIYGIAFTIEFNPDVFVPNSMSVEALNTSWMAAAIPGTSPA
                     LISINRSFAEDGFIEVAISRINRQNTGGQGVIAHLRGIIDDIAGITQSEIRITNIRAI
                     RADETPLAVYNPV"
     assembly_gap    1218..1317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1318..>1636)
                     /locus_tag="PK198_06620"
     CDS             complement(<1318..>1636)
                     /locus_tag="PK198_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38441.1"
                     /translation="DCCTDVVFDYSCPPCEILSLNVIPQPCNDDGTFDIVVGFDYLPA
                     NTLDSFLLTLSTGLEVEFALHNLPFTLPSVSADAGEEVIVRLRSLFNACDASATFTAP
                     DCDG"
     assembly_gap    1637..1649
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1650..>3130)
                     /locus_tag="PK198_06625"
     CDS             complement(<1650..>3130)
                     /locus_tag="PK198_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38442.1"
                     /translation="IPATLAPGVTFTITPGTPGGTVNSTGIMCLTGNIESLRIGGQNF
                     YMDEICHSPGLSQPCPAMNLQVEALSCTPNGLFYVAISPGVVASNTATGYRLLVNGEL
                     RGNYSYAQNTINLGPFTGDGVTPRTFIIEDNLNPACRDTFVLAPVTCTNNCGVADPQV
                     QIVDCSEGGYTLRINFQLVNPSAVGGFTVAVAGQIYGPFSMQQLPLILPNVQVPTDAL
                     EFAVQICPITIPAVVCCRTVMVTATPCPTDQCIGFENMTGSAYGASQGNQPGDLIYTE
                     NNVSVRLLPFQDSDWVTVFGELRVETAPGTPAFPAASGKFLSLRQIGLAFNFTQYPGP
                     VDTVTVQFFNQGPVNFAANGGPLFSLPLLTPGTYNIGSGVNMRVLLTGSSPQFGTLIF
                     SGNISSLRIGGANLRIDEMCVPEEPVCELGDATVIGTPACETPGDDRYVANIRIEGAQ
                     PGEMLIVRSVLTGQTDTIPYGNNALRVRFFHTDNNFDHLVICS"
     assembly_gap    3131..3367
                     /estimated_length=237
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3368..3570)
                     /locus_tag="PK198_06630"
     CDS             complement(<3368..3570)
                     /locus_tag="PK198_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38443.1"
                     /translation="MNARFIYVFLLAVWPVFAALFAQPLPTCVGFDDIPAGTYYNANY
                     GYEPGDVFFEGQGVTGRLARYIGS"
     assembly_gap    3782..3969
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3970..>4176
                     /locus_tag="PK198_06635"
     CDS             <3970..>4176
                     /locus_tag="PK198_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38444.1"
                     /translation="RIFPQFNERNCRRETIWNAVAPDRPYDFAQLALLFTYTRRLLDD
                     FLMQEQFWRQPDQQNLLLLSELRR"
     assembly_gap    4177..4220
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4221..>5244
                     /locus_tag="PK198_06640"
     CDS             <4221..>5244
                     /locus_tag="PK198_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38445.1"
                     /translation="RQTGSPTPDDGTAALSRYLLAAEADAYFTRAERRRNDESLQQKA
                     NLLDAFYLAEKLRDACEMQVRRHILKVDYSSRLLDAVLREVGDNITEYAHDPAIFLYY
                     HIYRMLSRDEESQRYFEALEALRQRQEVFDKTRLSELYNYFQNYCIRRINLGDERFLE
                     EIFKLYLILIEQELLYEDGLLSEWHYKNIITTGIRLRRLPWVRAFIESQKERLPPDAR
                     DNAYRFNLAAYHHAAGEYDAVLQLLLQVEYSDVRYSLGAKALLLRTYYELHEWEALDS
                     LVGSMRQYLQRNRLLADFNRNGYHNLFRFTRRAAAIRSQSAYLSDEKYHSELERLRRE
                     LSLAGEI"
     assembly_gap    5245..5359
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5412..6557
                     /locus_tag="PK198_06645"
     CDS             5412..6557
                     /locus_tag="PK198_06645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF38446.1"
                     /translation="MQYELSHWEYDSFFRTIDVAVLGSGIVGLSTAVRLKELQPSLNV
                     ALIERGALPLGASTRNAGFACFGSMTELLDDLRLRSEEEVWQLVEMRWKGLLRLRERI
                     GDAALAYEPLGGYEIFRASETSTFQECADHLSMFNQKMQSITGKADTYQIASEEKARS
                     FGFNDVPHLIWNSLEGQIHTGSMMAAWMRLAQQHNVLLLNGINIESIHPESDAVVLKT
                     SAGWDMRVPSLVVTVNGFAQRLLPELDVQPARNQVLVTKPIAGLSMRGAFHYDRGYYY
                     FRNVGPDRILLGGGRHLDLRGEMTDHFGTTSLIREKLLSLLDEMIAPGLHAEPERWWS
                     GIMGVGETKRPIVQKLHPRLTVAVRMGGMGVAIGTQVGEEAANLALG"
     gene            complement(6642..7211)
                     /locus_tag="PK198_06650"
     CDS             complement(6642..7211)
                     /locus_tag="PK198_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF38447.1"
                     /translation="MKSFITNFAAFMLVCAVFLLPSCGGETASGNASGESSAAPSAGQ
                     SAVQDDVSQKNIVGVAGNSPDHTTLVKAVVAAEYLDVLSNAGPFTVFAPTNDAFAKLP
                     AGTLEDLLKPENKDKLRRILEYHVTTSSLRADFLKDGMKLGMADGNSAQITVKDGKTQ
                     INGANIVGSVPASNGMIHVIDAVILAPAK"
     gene            7394..8065
                     /locus_tag="PK198_06655"
     CDS             7394..8065
                     /locus_tag="PK198_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38448.1"
                     /translation="MKFNLYLLLGVLLFAAACSSDQQPKIYGSFFVRYMEEGRQIKAH
                     ANFYEGDTLAAARPKIWTGGVSFLGSSMDVRDLQGSGMRYTAERQMDFMSEYMFRFTD
                     EKGKMQEIKMNAGSMAKAGFKEPVSKQAGLVFTYSGEKLKAGESIVILLTDEQNQTQT
                     IKFQGPQSSNDIILSNDTAASIPPGKWQMYAVRTGKTEMKQGRFDYLLESEYYTRTVV
                     VEVNP"
     gene            8130..8846
                     /locus_tag="PK198_06660"
     CDS             8130..8846
                     /locus_tag="PK198_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRF38449.1"
                     /translation="MKYLLNILLPLFVLTACEGPPTPRLARYSVHGIDVSHYQSRINW
                     AELDSLTVHFAFIKATEGISYADSLFERNWEALGATDIRRGAYHFFRPEAPPGEQARH
                     FVRTVTLETGDLPPVLDIELIGSLPKYELVRRLRVWLDIVENNMGVKPIIYTNLYFYN
                     RHLAGHFTGYPLWVARYHDREPILADGNDWAFWQYGKQGRLPGIRGHVDFNVFNGTLE
                     QLDSLRIPNPKVMELSERLR"
     gene            complement(8924..>9290)
                     /locus_tag="PK198_06665"
     CDS             complement(8924..>9290)
                     /locus_tag="PK198_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38450.1"
                     /translation="RRFSHASKDDLLPNAEQTLAPDGSLWQEIIYAYGNDGADSHVEV
                     YGRAGRLLEAMSLSFDAGRPMFHQNIPWILREWICIKCQSPPVELLQTHYKEDGSEEQ
                     EVICISQTTTAEGIPNASR"
     assembly_gap    9291..9304
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9305..9694)
                     /locus_tag="PK198_06670"
     CDS             complement(<9305..9694)
                     /locus_tag="PK198_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38451.1"
                     /translation="MPTTRKLIAPIFLLLLSFSGLALQSQSTRRIQSVSVDGKPAAWF
                     YYDKFGRLSRFYSSVEDERYEAPIFLKTHISYDRSGQLMSMSRFADMKGNTSFLFERR
                     DYRLDIYNRVVEEKVVVSDTITGRKMIP"
     gene            9788..>11125
                     /locus_tag="PK198_06675"
     CDS             9788..>11125
                     /locus_tag="PK198_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF38452.1"
                     /translation="MRQLLILFLLSCHAALAQAPLLDSALTYLHDRALFNGVVLLAEN
                     GKVVYQKNFGITDIRNPQPLQASSAFNLASVSKQFVCAMICLLQENGRLGFDDKVQKH
                     LPEFPYPDVSIRQLMNHTSGVPEYFDLVTRYSGSLDTIDNHDILRMLAEVQPPLDFVP
                     GERWQYCNTGYVMLGLLIEKLSGKSIADYFTEKITRPLGLKNTYIFHQKMKTSPANRV
                     YGFMREDGQNQLDDLMRVDGIVGDGNVYSSAEDLLKWSQALHGGRLLKSATMAEMMKP
                     ALLNNGNTSPYGFGLALNGCAQCAHHTGGWVGFLTLLWTDLEKKRTLVVLSNGGSSAG
                     IRAGQQFMKGQKMALPKTQLIKNVSLVDGTGAPARIASLRIKDDRIWETGELLPFKNE
                     PVFDGKGLTLAPGFIDTHSHHFSAADEDPTLLPALSQGITTIVIGQDGGSLAMD"
     assembly_gap    11126..11142
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11143..12291
                     /locus_tag="PK198_06680"
     CDS             <11143..12291
                     /locus_tag="PK198_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF38453.1"
                     /translation="RMERNPVAINAASYTGHTTLRLDAMGMGSFRRAATAAELEKMKS
                     QLNAELEKGSLGLCTGLEYERAFYSNRNEVMELAKVTAAAGGRYMSHIRSEDIHIEDA
                     IDEIISIGREAKLPVQVSHIKVAMKSKWNSSRLIIANMQAARSEGIDISADCYPYDFW
                     LSTLKVLFPNRDYDNLVSAEFACRELFDPAQSRLPAFLPQPAYKGLTVAEVARLRNET
                     PAQTLVWLIAEADRYDALHPDAEGRTEGIMGKSMLETDISHFLAWPHTNICSDGDPYS
                     HPRGHGAFPRVLAKYVREEKLLSLETAIHKMTALNAEHTGIQQRGLIAPGFFADLVLF
                     DPASIRDNATADQPTALSSGIGAVWVNGQLVFENGKATGMLPGRLVRR"
     gene            12360..12659
                     /locus_tag="PK198_06685"
     CDS             12360..12659
                     /locus_tag="PK198_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRF38454.1"
                     /translation="MSFSSILADIQGRADKASPIGSTIKFDFGDNQIIYLDGTGDTNT
                     VSQEDKWSDCLVNIKAEDMESLLAGKLNPMMAFMSGKIKVKGDMGVAMKLQSFLG"
     gene            complement(12685..13113)
                     /locus_tag="PK198_06690"
     CDS             complement(12685..13113)
                     /locus_tag="PK198_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF38455.1"
                     /translation="MLHGREAVAEEGGEDTFGFQADLTATYEFSEKFKLGLNVSDKTS
                     RTAELTTGGFFGSALYAGIGLGESFDLGLRGEYFLPKGVEKQDGIAAFTLSGNIRLGS
                     LTLIPEVRMDTSSADIFENVGNNAMTNQNTALLLAAVYAF"
     gene            complement(13044..13763)
                     /locus_tag="PK198_06695"
     CDS             complement(13044..13763)
                     /locus_tag="PK198_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF38456.1"
                     /translation="MRISVLNTCGVVVLLFSMYSYSFAQEAAPGGDIPAPEAAAEAEN
                     KISISGFIDGYYLHGFNQTGFGTSFTQRHSSFNLGMANVVFAKEGKVGFVADLAVGPR
                     AEEANGYAGSILSAIKQLYITYSPFEALKFTFGNFGTFIGYEVIDAPANTNYSMSYLF
                     SNGPFYHTGLKADLELGANFGVMIGLFDDTDRKFDEVPGKHIGGSALRWAGCRLTPMC
                     CMAARLWPKKAGRILSVFRPT"
BASE COUNT         3207 a         3595 c         3600 g         2941 t
ORIGIN      
        1 tctaccgggt tataaacagc cagcggcgtt tcatcggcgc gaatggcgcg gatgttggta
       61 atgcggattt cgctctgcgt aatgccggca atatcgtcta tgatgcctcg gagatgtgca
      121 atgacacctt gtcctcccgt attttggcga ttgatgcggc tgatggccac ttcaataaac
      181 ccgtcttccg cgaaggaacg gttgatagaa atcaaagcag gagaagtgcc cggaatcgcc
      241 gccgccatcc aggatgtgtt gagggcttcc acgctcatag aattgggtac aaagacatct
      301 ggattgaact caatggtaaa tgcgatgccg taaatagcct ccaccgacac cacggagcct
      361 cccagcaaaa tgggcgcaga gatttgcgtg ccgggggcaa tcgggccggc gggcatgtcc
      421 acaataatgc gcggattgtt gggcgtggcg ggcagcgggg tgtagggcgc taccgggccg
      481 tgcgtaagac catagttttc aatgagaacc tctacatcgc ccgtattgac gataccgttg
      541 ccgttgcagt ctgcataagc aaagttgaga tcattggtat tgaacatctg ctgccagggc
      601 gtcgcagcca ttcccgacca gtcggcggaa ttggcgctgc gcggcgggcc ttgcgtaccg
      661 aatgcaagac cgatgttgag caggtcgaag tggttggcga tgttgttgtt atcggtatcg
      721 ccgggccaaa cgatgatggg attgcaaacc gggcgctgaa cggtgataac ggctctgcat
      781 gtttcgggcg actcctgatc gcgcaccgaa aattcgagca caggcggcgt attcagtcca
      841 aacggtccca gtctgatggg caggtcatcg taatcaaatg tgccgtagaa gacgccgttg
      901 ccgcgcacgg taaaaccgtc ggagtggttg cctgtaggaa cgaagtcgag ttctacaaaa
      961 aactcatcgg catcgttgca atcggatgct accgcagtaa gctcggtaat gtgacaatcc
     1021 agatcgtcgc agttcaaggg gccgacctca atgtagccat agcaggccgg attgctgata
     1081 tcaaggatga ggatgtccac gatgttggtc ccgttgccgg ggaatggacc taccgtgacg
     1141 cccgcattgg agtagctgtg cggaccgctg atggcgccgt cggcaaagac gaaaaaccct
     1201 gcgctgccgg tattggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     1321 atcgcaatcg ggggcggtga aagtagcgct tgcgtcacaa gcattgaaca aagaccgcag
     1381 ccgaacaatg acctcttcgc ctgcatcggc agagacggaa ggaagggtga agggcaggtt
     1441 gtgcagtgca aattcgacct ccaaaccggt ggacaaggtg agcagaaagg aatcaagggt
     1501 attggcaggc aggtagtcga aacccactac gatatcgaaa gtaccgtcgt cgttgcaggg
     1561 ctgaggtatt acattcagac tgagaatctc gcaggggggg caagagtagt caaatactac
     1621 atctgtgcaa caatcgnnnn nnnnnnnnnt actacaaatg accaagtgat caaagttgtt
     1681 gtcggtatgg aaaaagcgaa cccgtaaagc gttattaccg taaggaattg tatccgtttg
     1741 tcccgtcaaa actgagcgca cgatgagcat ttcgccgggc tgtgcgcctt cgatccggat
     1801 gttggccaca tagcggtcat ctccgggcgt ttcacacgca ggagttccta tgaccgtcgc
     1861 atcgcccagt tcgcacaccg gttcttccgg cacgcacatt tcatctatac ggagattggc
     1921 gccgccaatt cgcaacgaag aaatatttcc tgaaaaaatc aaggtgccga attgaggcga
     1981 agaccctgtg agcaataccc tcatgttcac accagaacca atgttgtagg ttccgggcgt
     2041 cagcagcggc aaggaaaaca atggcccgcc gttggcggca aagttgaccg gcccctgatt
     2101 gaaaaactgc accgtaaccg tgtccaccgg gccgggatat tgtgtaaaat taaaagccaa
     2161 cccaatctga cggagagaca gaaacttgcc cgatgcagca ggaaaagcag gcgtgccggg
     2221 agccgtttct accctcagct cgccgaaaac cgtcacccaa tcggaatcct ggaaaggcag
     2281 caatctcacc gacacgttgt tttcggtgta gatcagatcg ccgggttggt tgccctgaga
     2341 ggcgccgtaa gccgatccgg tcatgttttc aaaacctata cattggtctg taggacaagg
     2401 cgttgccgtc accatcacgg tacggcagca caccacggcc ggaatggtga tcgggcaaat
     2461 ttgtacggca aattccaacg catcggtagg cacctgcaca ttgggcagga tgagcggcaa
     2521 ttgctgcatg gagaacggcc cgtaaatctg gccagccacc gcgacggtga atccgccgac
     2581 agcactgggg ttgaccaact gaaaattgat gcgcagtgtg tagccgcctt cagagcaatc
     2641 tacaatctgc acctgtgggt cggccacacc gcagttattg gtacaagtca ccggcgccag
     2701 tacgaaggtg tcgcggcagg ccgggttgag gttgtcttct atgatgaagg tgcggggggt
     2761 aacaccatca ccggtgaaag gtcccaaatt gatggtattc tgggcgtaag agtaattgcc
     2821 ccgaagctcg ccgtttacca acagccggta gccggtagcc gtattggagg caaccacacc
     2881 cggcgaaatt gccacataga acaagccatt gggcgtgcaa gacagtgctt ccacctgcaa
     2941 attcatggct gggcaaggtt gcgacaagcc cggcgagtgg caaatttcat ccatataaaa
     3001 attctgtccg ccgatgcgca aagactcaat gttgcccgtc aggcacatga tcccggtgct
     3061 gttcaccgtg ccgccgggcg tgccgggcgt gatggtaaag gtgacgccgg gcgccagcgt
     3121 ggcaggaatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnngag ccgatgtagc gggccagccg cccggtaacg ccctgtcctt caaagaagac
     3421 atcgccgggt tcgtagccgt agttggcatt gtaataggta cctgccggga tatcgtcaaa
     3481 ccctacgcag gtgggcaatg gttgtgcaaa aagcgctgcg aaaacaggcc acacagccag
     3541 aagaaaaacg tagataaatc ttgcattcat ggttatcggc ttttttaatt tgaattgcat
     3601 gatacaaaga ttcagcgaaa acatcttctt cgcaaaaact ttataccgcc gtttttgatt
     3661 tttttcaccc tgcaaacata gaaatatttt tttataaaaa attggtttac aattattaaa
     3721 aacagaatac cttggcactc tgatttgatt ttttttaccc aaatggagca tgataaacag
     3781 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnng ccgaatcttc ccgcagttca acgagcgcaa ttgccgccgg gaaaccatct
     4021 ggaacgccgt tgcccccgac cgcccctacg actttgcaca gctcgcactc cttttcacct
     4081 acacccgccg cctgctcgac gactttctga tgcaggagca gttttggcga cagccggacc
     4141 aacaaaatct actgttgctc agcgaattgc gccgccnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn tcgccaaacc gggtcgccca ctcccgacga cggcacggct
     4261 gcgttgtcca ggtacttgct tgccgccgaa gccgacgcct atttcacccg tgccgagcgc
     4321 cgccgaaacg acgaaagtct ccagcaaaaa gccaacctct tagacgcgtt ttacctcgcc
     4381 gaaaaactac gcgacgcctg cgaaatgcaa gtgcgccgcc acatcctcaa ggtggactac
     4441 tcctcgcgcc tgctcgatgc ggtgctgcgc gaggtgggcg acaacataac cgagtacgcg
     4501 cacgatccgg ccatttttct gtactaccat atttacagga tgctcagtcg ggacgaggaa
     4561 tcgcagcgct atttcgaggc attggaagca ctgcgacaac ggcaggaagt ttttgataaa
     4621 acccggctgt cggaactgta taattatttt caaaactact gcatccggcg catcaacctc
     4681 ggcgatgagc gttttttgga ggaaattttc aaactgtacc tcatcctcat cgaacaggaa
     4741 ctgctgtacg aagacgggct gctctccgaa tggcattaca aaaacatcat taccaccggt
     4801 attcgcttgc ggcggttgcc ctgggtacgc gctttcatcg aatctcaaaa agaacgcctg
     4861 ccccccgatg cccgcgacaa tgcctaccgt ttcaatcttg ccgcctacca ccacgctgcc
     4921 ggcgagtacg acgcggtgtt gcaactcctt ttgcaggtag aatacagcga tgtgcggtac
     4981 agcctcgggg ccaaagcgct gctgttgcgc acctattacg aattgcacga gtgggaggcc
     5041 ctcgactcgt tggtaggatc tatgcggcag tatttgcagc gcaatcgcct gctggccgat
     5101 ttcaatcgaa acggctacca caatttgttc agattcacgc gccgcgcggc tgccatccgc
     5161 tcccaaagcg cctacctctc agatgaaaaa taccactcgg aactggagcg cctgcgccgg
     5221 gaattgagtc tcgccggaga aattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnng agtgcgtgag gggtgagtga cttatattgg caggctaaat
     5401 attttctcat tatgcaatac gaactcagtc actgggagta cgactccttt tttcgcacca
     5461 tagacgtcgc cgtgctcggc agcggcatcg tgggtttgag cactgcggtg cgcctcaaag
     5521 aactgcaacc ttcgctgaac gtggccctca tagaacgggg cgcgctgccc ctcggcgcca
     5581 gtacgcgcaa tgccggcttt gcctgcttcg gcagtatgac cgaactcttg gacgatctcc
     5641 ggctgaggtc ggaagaggag gtctggcaat tggtggaaat gcgctggaaa ggactgctgc
     5701 gcctgcggga acgcatcggc gacgcggcgc tggcgtatga gccgctgggc gggtacgaga
     5761 ttttcagagc ctccgaaacc agcacttttc aggagtgcgc cgaccacctc agcatgttta
     5821 atcaaaaaat gcagagcatc accggaaagg ccgatactta ccagattgcc tcggaagaaa
     5881 aggcccggtc tttcgggttc aacgacgtgc cgcacctgat ctggaattcg ttggaaggtc
     5941 agattcatac cggcagtatg atggcggcct ggatgcggct ggcacagcaa cacaatgtgc
     6001 tgttgctcaa cggtatcaac atagaatcca tccaccccga atcagatgcc gttgtgctca
     6061 aaaccagcgc cggctgggat atgcgcgtac cttctttggt ggtgacggtc aatgggtttg
     6121 cccaacgttt gttgccggag ttggacgtgc agcccgcccg caatcaggtg ctcgttacaa
     6181 agcccattgc cggtttgtcc atgcgcggcg cctttcacta tgaccggggt tactattatt
     6241 tcagaaacgt agggccagat cgcattttgc tcggcggagg gcggcatttg gacctccggg
     6301 gtgaaatgac cgatcacttc ggcactacct cgctcattcg ggaaaaactg ctcagcctgc
     6361 tcgacgaaat gattgcgccc ggcctgcacg ccgaaccgga acgctggtgg agcggcatta
     6421 tgggcgtagg agaaacgaag cggcccattg ttcagaagtt acatccccgc ctgacggtgg
     6481 ccgtcagaat gggcggcatg ggcgtggcca tcggcacgca ggtgggggaa gaggcggcaa
     6541 acttggcact aggttgagtc aggcttagtg aagggatgac tacggataaa aatctgacgg
     6601 agtcgcccct tcactatgca agcaggtact ggctgtacga tctatttggc cggagccaaa
     6661 atcacggcat caatgacgtg aatcataccg ttcgaggccg gtacgctgcc cacgatattg
     6721 gccccgttga tctgcgtttt tccatctttt acggtgattt gagccgagtt gccgtcggcc
     6781 atgccgagtt tcatgccatc tttcaggaaa tctgccctca gcgaggaagt tgtgacgtga
     6841 tactccagaa tgcgacgcaa tttgtctttg ttttcaggct tcagcaggtc ttccaaagta
     6901 ccggcaggca gtttggcaaa ggcatcattt gtgggtgcaa aaaccgtaaa cggccccgca
     6961 ttggacagca catccaagta ctcggcagct actacggctt tgaccaaagt agtatgatcg
     7021 ggcgagttgc cggctacgcc cactatgttt ttttgtgaaa catcgtcctg cacggcagac
     7081 tgaccggcag acggagcagc gctgctttcg cccgacgcgt tacccgatgc tgtttcgcct
     7141 ccgcaagagg gcaacaaaaa aacggcacag accagcataa aagctgcaaa attggtgatg
     7201 aaggatttca tgatgatagt tatttggttt gaaaatgaat aacttgtgcc tacacacaat
     7261 taagacctaa aggtatttta ttcaaaacta acagccaaat tttttatgtc atttttttga
     7321 cattctgctc aaagccggcg ctggtcggat ttcaaactgc gattcctgtt atctttgccg
     7381 gaaattccac gcaatgaaat tcaatcttta ccttttgctt ggcgtccttt tgttcgcagc
     7441 agcctgctcg tccgatcagc agccaaagat ttacggcagt ttttttgtca gatacatgga
     7501 agaggggcgg caaataaaag cccacgccaa tttttatgaa ggcgacacct tagccgctgc
     7561 ccgccccaaa atctggaccg gcggcgtctc ttttttggga agttcgatgg atgtgcgcga
     7621 tttgcaaggc tccggcatgc gctatacggc tgagcgtcag atggatttta tgtctgaata
     7681 tatgtttcgg tttaccgacg aaaaaggcaa aatgcaggag atcaaaatga atgccggcag
     7741 catggcgaaa gccggattca aagagccggt atccaaacag gcgggcttgg tatttaccta
     7801 ctccggcgaa aaactgaaag ccggcgagag catcgtcatc ctgctgacag acgaacaaaa
     7861 ccagacccaa acaattaaat ttcaggggcc tcagtcttcc aacgacatca ttctgagcaa
     7921 cgacacagcc gcttcgatac ctcccggaaa atggcagatg tatgcggtga gaaccggcaa
     7981 aacggagatg aaacaaggcc gtttcgacta cttattagag tcggagtatt atacccggac
     8041 ggtggttgtt gaagtgaacc cataaaaccc acggtaattt ccggcacgga ctttgacggt
     8101 agaaaatttt tgtgcaaatt gcagacggaa tgaagtatct cctgaacata ttgctgcccc
     8161 tattcgtgct gaccgcctgc gaagggccgc ctacgccgcg cttggcgaga tattccgtgc
     8221 atggcataga tgtatctcat tatcaatccc gtatcaactg ggccgaactg gactccctca
     8281 ccgttcattt tgcattcatc aaagctaccg aaggcatcag ctatgcagac tcgctgtttg
     8341 agcgcaactg ggaagctctg ggcgctaccg acatccgccg gggcgcttac catttcttcc
     8401 gtcccgaagc gccgcccggt gagcaggccc ggcattttgt gcgcaccgtc accttggaaa
     8461 ccggggattt gccgcccgtg ttggacattg aactgatcgg cagcctcccc aaatacgaat
     8521 tggtgcggcg gctccgggtg tggttggaca tcgtggaaaa caacatgggg gtgaagccca
     8581 tcatttatac caatctgtat ttttacaacc gccacctggc cggccatttc accggctacc
     8641 cgctctgggt tgcccgctac cacgaccgcg aacccattct ggccgacggc aacgactggg
     8701 ctttctggca atacggcaaa caggggcggc tgcccggcat tcgcgggcat gtggatttta
     8761 atgtgttcaa cggcactttg gagcagttgg attcgctgcg cattcccaac ccgaaagtga
     8821 tggagttgag cgagcggttg cggtgagggc agggcttctg tccgtactca atcctccaca
     8881 taataaaaat caaaaacgac gctgctgccc ttcgtctcat tgatcaccgt gatgcgtttg
     8941 ggataccctc tgccgtcgta gtctgtgaga tacaaataac ctcctgttct tcgctgccgt
     9001 cctctttata atgagtttga agcaattcga caggcggcga ctggcatttg atgcaaatcc
     9061 attcgcgcaa aatccaggga atgttttgat gaaacatggg gcgccccgca tcgaagctca
     9121 acgacatggc ctccaacaag cgcccggccc ggccatacac ttcgacgtga gagtcggctc
     9181 catcgttgcc gtaagcgtag ataatttcct gccaaagact tccgtcaggg gctaatgttt
     9241 gctcggcatt cggcagcaaa tcgtctttgg aagcatgcga aaaccggcgg nnnnnnnnnn
     9301 nnnnaggaat cattttgcgg ccggtgatcg tgtcggatac cacgactttt tcctcaacga
     9361 cccggttgta gatgtccaaa cggtagtcgc ggcgttcaaa caaaaacgag gtattcccct
     9421 tcatatcggc aaagcggctc atggacatca gttgcccgct gcggtcgtag ctgatatgag
     9481 ttttgagaaa tatcggggct tcgtagcgtt catcctccac cgaactgtaa aaccggctga
     9541 gcctgccgaa tttgtcgtaa taaaaccaag ctgccggctt tccgtccacg gacacagact
     9601 gaatgcggcg ggttgattga gattgcaggg caaggccgct gaaactgagc agcagcagga
     9661 agataggggc aatgagcttg cgggttgttg gcatggattt cgtttgtgtg gcaaagatag
     9721 gctgtttttt gcaacacagc gcctcagaaa aaatccgttt cttgggccaa attttccatc
     9781 catgaaaatg cgtcagcttc tcattctgtt cctgctctcc tgccatgccg cgctcgcaca
     9841 ggcgcctctg ttggacagcg ccctgaccta ccttcacgac cgcgccctgt tcaacggcgt
     9901 agtgctgctg gccgaaaacg gcaaggtggt ttaccaaaaa aacttcggca tcaccgacat
     9961 ccgaaaccct caaccattgc aggcatcttc ggccttcaac ctcgcctctg tttccaaaca
    10021 gtttgtctgt gccatgatct gccttttgca ggaaaacggc cggctcggct tcgacgacaa
    10081 ggtgcaaaaa cacctaccgg agtttcctta ccccgatgtg agcatccgcc aattgatgaa
    10141 ccacaccagc ggcgtgcccg agtatttcga cctcgtcacc cgctactccg gctctttgga
    10201 caccattgat aatcacgata ttttgcgcat gttggcagaa gtgcaaccgc cgctcgattt
    10261 tgtgcccggc gagcgctggc agtactgcaa cacgggctat gtgatgctcg gtttgctcat
    10321 cgagaaattg tcgggcaaaa gcattgctga ttattttacc gaaaaaatca cccgcccgct
    10381 gggattgaaa aacacctaca tttttcatca gaaaatgaag acctcgcccg ccaacagggt
    10441 ttatggtttc atgcgggaag acggccaaaa tcaactcgac gacctgatgc gggtggacgg
    10501 cattgtgggc gacggcaatg tttattcgtc tgccgaagat ttgttgaaat ggtcgcaggc
    10561 gctgcacggc ggtcgtttgc tgaaatccgc cacgatggcc gaaatgatga aaccggcgct
    10621 gctgaacaac ggcaacacct cgccctacgg gtttggtctg gcgctcaacg gctgcgcaca
    10681 gtgcgcccat cacaccggcg gctgggttgg gtttttgact ttgctttgga ccgatttgga
    10741 aaagaaacga actttggtgg tgctgtccaa cggcgggtct tctgccggaa tccgggcggg
    10801 acagcaattt atgaaaggac aaaaaatggc gcttccaaaa acacaactca ttaaaaatgt
    10861 gagccttgtg gatggtaccg gcgcacctgc ccgcatcgct tccctgcgca tcaaagacga
    10921 ccggatttgg gaaacaggcg aactgctgcc tttcaaaaac gaacctgttt tcgacggcaa
    10981 gggcctcacg ctggcgccgg gctttataga cacccacagc caccatttca gcgccgccga
    11041 cgaagacccc acgcttttgc cggcgcttag tcaaggcatt accaccatcg tcatcggaca
    11101 agacgggggc agcctggcga tggacnnnnn nnnnnnnnnn nnagaatgga aagaaatccg
    11161 gtagccatca acgcggcctc ttacacgggg cataccacgc tccgcctcga cgccatgggc
    11221 atgggtagtt tccggcgtgc tgcaacggct gccgaattgg aaaaaatgaa atcgcaactg
    11281 aacgccgaac tcgaaaaagg ctcgctcgga ctgtgtacgg gcctggagta cgaacgggcc
    11341 ttttattcca accgaaacga ggtgatggaa ttggccaaag tgactgccgc agccggcggg
    11401 cgctacatga gccacattcg cagcgaggac atccacatcg aagacgccat tgacgagatc
    11461 atcagcattg gccgggaagc gaaattgccc gtgcaggtct cccacatcaa agtggccatg
    11521 aaaagcaagt ggaactcgag ccggctcatc attgccaata tgcaggcggc ccgcagcgag
    11581 ggcatagaca tcagcgccga ttgctacccc tacgatttct ggctttcgac gctcaaagtg
    11641 ctgtttccca atcgggacta cgacaatctg gtgagcgccg aatttgcctg ccgggaactc
    11701 ttcgatccgg ctcaatccag gctgccggct tttttgccac agcccgctta taagggtctg
    11761 accgtggcgg aagtagctcg cctgcgaaac gaaacgcccg cccaaaccct cgtctggctc
    11821 attgccgaag ccgaccgcta cgacgcactg caccccgatg cggagggccg aacagagggc
    11881 atcatgggca aatccatgct ggaaaccgac atttcgcatt tcctggcctg gccgcacacc
    11941 aatatctgct ccgacggcga tccgtacagt caccctagag ggcacggggc ttttcctcgg
    12001 gtattggcga agtatgtgcg ggaagaaaaa ctgctctcgc tggaaacggc catccacaaa
    12061 atgacggctc tgaacgccga acacaccggc attcagcagc gcggactgat agcgccgggc
    12121 ttcttcgccg atctggtgct gttcgaccct gcctccattc gcgacaatgc caccgccgat
    12181 cagccaacgg ccctgtcgag cggcatcggc gccgtgtggg taaatggtca attggtgttt
    12241 gaaaacggca aagccaccgg gatgctgccg gggcgcttgg tgaggaggtg aaggcagaca
    12301 aacaatgtgt ctttcccact cgttttgtgt atcttttatg aattcaatcc attccaacca
    12361 tgagtttttc atccattctg gccgatattc aaggccgcgc cgacaaggct tctcccattg
    12421 gttctaccat caaatttgat ttcggcgaca accagatcat ctacttggac ggcaccggcg
    12481 ataccaatac cgtctcgcag gaagacaagt ggtcggactg cttagtgaac atcaaagctg
    12541 aagatatgga gtctttgctg gcgggcaagc tcaaccccat gatggccttc atgagcggca
    12601 aaatcaaagt aaaaggggac atgggcgtgg ccatgaaact acaaagtttt ttgggatgag
    12661 ccacgcccga tgtccgggaa tcctttagaa agcataaaca gcggccaaga gcagcgctgt
    12721 attttggttg gtcatggcat tattccctac attttcaaaa atgtcggcag aggaggtgtc
    12781 catgcgcacc tcaggtatca gcgtgagact gcccaaacgg atattgcccg aaagggtgaa
    12841 agcagcgata ccatcctgtt tttcaacacc tttggggagg aagtactcgc cccgcaaccc
    12901 cagatcaaag gactcgccca gaccgatgcc tgcgtacagc gcactgccga aaaagccgcc
    12961 ggtagtgagc tcggccgttc gggaggtttt gtcagacacg ttcaaaccta atttgaactt
    13021 ttcagaaaac tcgtaagtgg cggtcaggtc ggcctgaaaa ccgaaagtat cctccccgcc
    13081 ttcttcggcc acagcctcgc ggccatgcag cacattggcg taagccgaca gcccgcccaa
    13141 cgaagcgcgg aaccgccgat atgtttgccc ggaacctcat cgaacttccg gtcggtatcg
    13201 tcaaaaaggc cgatcatcac gccgaagttg gcgcccagct cgagatcggc tttgaggccc
    13261 gtatgataaa aaggcccatt actaaacagg tatgacatgg aataattcgt attcgccggt
    13321 gcgtctatca cctcgtatcc tataaaagtg ccgaagttgc cgaacgtgaa cttcagcgcc
    13381 tcgaaaggcg aataggtgat gtacaattgc ttgatggccg acaaaataga gccggcgtat
    13441 ccattggctt cctcagcgcg gggacctact gccaggtcgg ccacgaaccc cacttttccc
    13501 tctttggcaa agaccacatt ggccatgccg agattgaagc tgctgtgcct ttgggtaaaa
    13561 ctcgtcccga agcccgtctg attgaagccg tgcaggtagt agccgtcaat gaagccggaa
    13621 atactgattt tgttctctgc ctctgcagcc gcttcgggcg ccggtatgtc tccgccgggc
    13681 gctgcttcct gcgcaaaaga gtaggaatac atggagaaca ggagcactac tactccacag
    13741 gtgttgagta ccgaaatacg catggttgaa atttttgagt atgaaaaaat cgtttcaact
    13801 tgcgataacg gcttctcata caatacctta gccaacttca tctgggcgaa agcccggaat
    13861 aaacgggccg tcgcagatga acaatcagcg ttgatattgt atttttgctt ttacaacaga
    13921 gaagcgggaa accgttatct cgtgctcccg tcagcgttgc cgcgctgttc gggagttttt
    13981 tttgcataga gcacagggca gagagcatag tgaaactgcc ccatgcttta ttatttcacc
    14041 aattccagca tcgggtccac cgtgtcggag t
//
LOCUS       DAORLF010000054        14009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2322_length_14009_cov_1.983876, whole genome shotgun sequence.
ACCESSION   DAORLF010000054 DAORLF010000000
VERSION     DAORLF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_14009_cov_1.983876"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>216)
                     /locus_tag="PK198_06700"
     CDS             complement(<1..>216)
                     /locus_tag="PK198_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38457.1"
                     /translation="NTTMGAPPTGVLLDGITQDSIFVIRLEASNLCGVRIAEAPVLVR
                     PYPIVRFGVFPNEGCSPLQVALSNATV"
     assembly_gap    217..361
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<362..1069)
                     /locus_tag="PK198_06705"
     CDS             complement(<362..1069)
                     /locus_tag="PK198_06705"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF38458.1"
                     /translation="MRDTIMVVVTQPQVLSLNPDTAVCISDQFLQLQTNLSGGQWSGP
                     GTDPAGRIDLSVAGGNQTHTYRYEFALGTNCEQTGTVQVQIIDLGALVNAGPDVSLCQ
                     GPATYALSGNTPQPGYWVGQGVNPSSGVINLAQLNLDTTYIFQYCIESQSVAGCMACD
                     SRSLILYSNPVAAFSLDGTACIGETFQVQNTGTGADAYSWDFGDGTPVLNTASPEHQY
                     GMQGNYTIQHIAVNTATT"
     assembly_gap    1092..1192
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1193..2006)
                     /locus_tag="PK198_06710"
     CDS             complement(<1193..2006)
                     /locus_tag="PK198_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38459.1"
                     /translation="MQILPAVTASIAAVPPACDSLNYTPMVSYTGAINTYNWSFPGGN
                     PASSTQANPVGIRYSVPGTYTAILEASGACGFVRDTVQIVVQTNVPLSITPPPGPLCS
                     GSSPLTLTANEEGGTWSGNGITDAVQGIFNPAIVTPDQPVNIQYSITNGACSASAGIV
                     LTVVASTSVSFPADTFCIDSAPRMLLVNPSGGTFTGAGITDTTTGIFAPMNAGINLHE
                     IGYAYTDANGCRVNAISQVLVEGLPQINLPDTTLLCLSSISIDLPATVGFAPA"
     gene            complement(2090..>2621)
                     /locus_tag="PK198_06715"
     CDS             complement(2090..>2621)
                     /locus_tag="PK198_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38460.1"
                     /translation="ADTTRWRFADTTMNLNTNNIVVNFLRPGTYTVRQRAFNACGEDF
                     ETVTINIYQPPSISLTAPTPSCDQITLSSANLGFSHSGEITNFQWTFTNGTPASATGP
                     SFSGVTFTQSGSITLQITSPCGRPKPYLWWWPVRSLLFSIRPIRHRCVPMASPLSCEL
                     LRPAESGRGLVCPTIL"
     assembly_gap    2622..2636
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2637..3389)
                     /locus_tag="PK198_06720"
     CDS             complement(<2637..3389)
                     /locus_tag="PK198_06720"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF38461.1"
                     /translation="MRALLILLFLALFSHFGWSQCLPQPVLCNQNQCAFPNVNLTLSV
                     DGNNEFCDGDTIHLEINESQSLDFDYLIYYWCDGNVDTITFQDQPGTHVYIVPDSLLC
                     QSAESNYTIWVIGIKSCANGITCRSAATTLTVKNRPVANFNVQGEVCVNTSVPFSNAS
                     CNATTYLWNFGDGTTSTLQNPSHTYATPGNYTVSLTVTNTCGSDTQTRPVRVVAPPEA
                     AFTHNPAFGCGPTTISFTDQANQWSNTVWSITP"
     gene            complement(3401..4576)
                     /locus_tag="PK198_06725"
     CDS             complement(3401..4576)
                     /locus_tag="PK198_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF38462.1"
                     /translation="MLPKILLLPLLLCATSLVAQDPFFTHFFNNRSLFNPALTGETGA
                     LSLRALYKSQWHLGGDIPYRTMAATLEESMPCSWFDLGLNVVQDVEGDGRLSTFSGGF
                     MLAGTPTLEVRDAIHNLRIGVGFNWAQRRIDFSRLTFSDQLDPKYGFFDVNGNPNPTG
                     FVPPDFTGSFWYFMPSVGVAYKALFNKTNRHLGVFSIGYSMHNLLGLGDANVFGHQES
                     VLRIGTTTVPRHTAFAEWEFVPYYSAQTYLSVKPTVLWQQQGGLTYLEAGIQAGISRM
                     VAAGAYYHFGRRPPSGVNTNWLSFNVETSVLTTTGNRNTSRFDLGLSFSPNISGLQNT
                     VGVGSIVEVSVAVHFAASPGCKFMGREDEVSYGKLPCPTAAQSRRSKIYENIWYGKK"
     gene            complement(4799..>6022)
                     /locus_tag="PK198_06730"
     CDS             complement(4799..>6022)
                     /locus_tag="PK198_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38463.1"
                     /translation="QLPPPTETPPVPWWKKYRGAMIWFAAAFVMMILLYKWLDKNPEE
                     VIQATQTTLPRSPSDPSNETVQPAEDTTKYPPQRAEQNTANGRKAAPIEVQRVSDPET
                     EPRNSSDSTSVPAHTAWADTVENKNNDILIPDRTVPNPATPKKTFDRTVVAMPPVRYA
                     SNDFSETAGKVDFKLRTQMFNSNRVTLLSEPLANADLEQGLLGLKEYNQDAILRQHNV
                     EYVVSAALRGRSIDFIKFPTTGTSSYFCKLTVELSILDAKTYDIKATYVLKNPEYRNI
                     DSNRGQTFMSPQAAENAAVEAIGKELQEVLKTFFPLHGWVIEVREQDNREAKTLLVDI
                     GNKQSVQKGDVFNVVYERRLAAGTGGIDAISEIGEVVIKEVYGVTAIAIVRRGGAEIL
                     RLMKEDATLFVRSKN"
     assembly_gap    6023..6186
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6244..6825)
                     /locus_tag="PK198_06735"
     CDS             complement(6244..6825)
                     /locus_tag="PK198_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF38464.1"
                     /translation="MKNIFNFTRCMQTWTDDQIKAALRKGGYHRERALEYMFKAWFSA
                     LRDEAKRTGLGQEDAEEIAADAIWALMKQILNTQRGEINYLKTYAVKIAKRMAWRKKT
                     AIAYDVIDLDEIKELTGGEPSGGIDKILRWKKVENELEKMGEPCLSILLMRAWGYRTD
                     EIASHTDLAESTVKNKTRPCREELARRIEENLS"
     gene            complement(6838..7062)
                     /locus_tag="PK198_06740"
     CDS             complement(6838..7062)
                     /locus_tag="PK198_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38465.1"
                     /translation="MPGIIIEKALELCKDFSAFSPAEKAEIAAICEFREYKAGQNVFS
                     IEHQETYCYQHFPDWAGVGRLIEHNDAANA"
     gene            complement(7172..>7396)
                     /locus_tag="PK198_06745"
     CDS             complement(7172..>7396)
                     /locus_tag="PK198_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38466.1"
                     /translation="EFIAFIKEKYSYYQNLPAILEINNRAFDSASKEVFRGLSSCIET
                     IKQNSQTWDRDATPVLSKTYIAMERLLQTF"
     assembly_gap    7397..7464
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7465..9872)
                     /locus_tag="PK198_06750"
     CDS             complement(<7465..9872)
                     /locus_tag="PK198_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF38467.1"
                     /translation="MKDFFLWFILFLFINTYCHGQQFGTTVITHGYQLTGVAPMRGEW
                     MYNMAKAILEKTGGRGCICTYDKISGLFKTEEGSCLNGEVILLFDWAAESNINNAGFT
                     EAAGDLLFASLVRGLLRQQFTLHQIHFIGHSRGAVVNTEAIERLITLKNTYPSFRNII
                     SVDHVTNIDPHDWGILGDYTDFDNHPNLNITCPSNDKPNNGVISWSGSGFNDTYFQTN
                     INSILNGRAVDGTYNCDWSQHLQGHTEIQQSYITSIRTFSTGVSTPCGASGYRFSRIG
                     GETRPLTSLLCDPNMHKYSTEFDFFDPMYIISNQLRRVRGIMNGSFDRQFEKIDINSR
                     TGSINTIRHLPGWQIVNANPVKDILSNSGIVIEAVSGRSSSVIHNWLFVPPDVKALRI
                     NLKKSSSNTGSIILKAGAEMEVSFRDTLDTSQDLFTDVYFDISRFQGRIIKFSIEVAA
                     GSSANTFSIMLKEVAFSKQIPQNLPTLFLFDLSGSMNERMSSGVSRIEEAKAAAKATL
                     RTMGNATNQGVSQEIGIRGFSGACVSDPTFPITNGFTTDFSAVENAVNRIPIPNGGTP
                     LFEAIQSSRQKLLQRLDQTGAKQAKLIILSDGQATCTPIRPADVYAFGQYGQTTQNIS
                     TSGNPLLSSNLRYYAVGFGIDPGSPAERDLQYLAQISGGKYLNAQNQFELTRAFQKFN
                     KIFVPKPAPALVDVPAASRDVFDRGVSTIYAEEYETALERCKTYVQQHPEDCHGIYNF
                     ALMQEANEYYKSAIASFEKYLQLCPGADDGIHVRKQIADLKQDYEAFLIFNRKVIMSD
                     MEYL"
     gene            complement(9982..11541)
                     /locus_tag="PK198_06755"
     CDS             complement(9982..11541)
                     /locus_tag="PK198_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF38468.1"
                     /translation="MKKIIYFYSALVIVAVFLSFETAWGQKALKGIFKDALVLDFSDP
                     YSHITIDYEEDEIIVPVSASNKALSKQKKNINLKRIIPGSIVDIEFEVVNNERVATVI
                     KTDLEDDGVVKLEGLLELIEDDIAYVDGQKVKLASNVSITGKTNADCDCKGKVFTSFK
                     DPLLKTGKYFLEIKGKLNDDDGVIIAEEVRACKNLLLETDAKLRNAVEQSYNTSGLAM
                     VSTPTDIGLSVGLPLHQGNIRIGQYSYKLHNDIRLQGYINSVGERVIPEHQKNLPDND
                     PNKLNFRFYVIDNPIPNAFAFPNGMIFVHTGILDVMENEAELAIVLGHEVSHVTHEHG
                     RERYESSVLTGIMANIGEGLLMKFTRNSNLSPLLANAVKQTYAALRPEALANIFGAQP
                     AKESQADRVGLQYALLAGYDIREAVGFWGKMAKITNDGNFKSRLNNDFRSMLSSADFS
                     YGNPLNTLGEAGFGMLAKMFLDTVYTSHPKSKARARALNQLILTSYKNEDFEHLDKGA
                     AMFEKYVKGVR"
     assembly_gap    11592..11616
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11679..12641)
                     /locus_tag="PK198_06760"
     CDS             complement(11679..12641)
                     /locus_tag="PK198_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38469.1"
                     /translation="MKKIVFFILLAASFYSNFVLAQETLGTVSDQARISLTPVVPDAS
                     DNLPLEARPMLLGRLRQLANTQGFSGDAVFPQFILAANPILIDKKVTGTAPTKILIEL
                     EVNLYIADRNTRTIFSTTSAAVKGVGSTDIESYQSAFRSLRLDNSAMKNFVSEGRNEI
                     IKFYNTRCDLLMNRVDMLAKANQADEALEMLLQISDVSQPCAEKATAKTEQVFEAAAA
                     QRCQALLQAAKTAWAAEPTPAGAQKAVTFLSILPPGSKCNTEGTALLSDIRAQMKENV
                     SWERTQYKDRIDLMRRYIQAIRDIGVAYGNGQPDTQIIVKGWLW"
     gene            complement(12692..13666)
                     /locus_tag="PK198_06765"
     CDS             complement(12692..13666)
                     /locus_tag="PK198_06765"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HRF38470.1"
                     /translation="MKISPTYLLIAMFMMINVAAYAQAKKPSLMVVPSDIYCNKNGFM
                     TVIDDQGRKIKVPDYQRLFLEDRNMRAIISAINNFMNDQGFPLIDMEATLKNLDIEDA
                     EESVMYDKSGEELTESLLDKIKRRAKADIILDVDFAFTQTGPIKTMTFNLKGIDAYTN
                     RQIAGVEGAGKPSANANIEIMLREDNYTRMTEFSATLNDYFEKLFKEGREVSIVIRLS
                     KGASIDFDTEYGDEEFSEILDNWFNKNTFREAGNQNGRYSIGDMSDVRMPVIAKIPVY
                     DAKGKAIDAREFAQQLRKFLKTAPYHLDATVRTRGLGQAHVIIGANKG"
     gene            complement(13701..>14009)
                     /locus_tag="PK198_06770"
     CDS             complement(13701..>14009)
                     /locus_tag="PK198_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38471.1"
                     /translation="IAAKKNAVQELLFKGIKASTNPGCPSRPIIDDISKRNSDYFNDF
                     FKNNGAYLQFVNLSGDQTPTRVKVRNLIKVSIDVIVDHKRLQKQMVDDKQTRGLNDGF
                     "
BASE COUNT         3391 a         3145 c         3114 g         3841 t
ORIGIN      
        1 caacggtggc attggacagg gctacttgca gcggcgaaca accttcattg ggaaacacgc
       61 caaaacgaac aatgggatat gggcgcacca aaaccggcgc ttctgctatg cgcactccgc
      121 aaaggttgct ggcttccagt cgaattacaa aaatggaatc ctgtgtaatg ccgtccaaga
      181 gtacgcccgt cggcggcgcg cccatagtgg tatttcnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nggtggtggc cgtattgacg gcaatatgct gtatggtata gtttccttgc attccatact
      421 gatgttcagg ggaggcggtg ttcaacaccg gggtaccgtc gccaaaatcc cacgagtatg
      481 catctgcccc cgtgccggta ttctgcacct gaaatgtttc gccgatgcag gcagtaccgt
      541 caagagagaa cgcggctacc gggttggaat acagtatcag gctgcggctg tcgcaggcca
      601 tacaccccgc taccgactgg ctctcaatac aatactggaa aatataggta gtatcaaggt
      661 tcagttgcgc aaggttgatt accccgctgg aaggattgac accctgtccc acccaataac
      721 ccggctgcgg agtattgccg ctcaaagcat atgtggccgg cccctgacac aacgacacgt
      781 ccggaccggc attcaccaat gcgcctaggt caatgatttg cacctgtact gtgcctgttt
      841 gttcgcagtt ggttcccaat gcaaattcat aacgatatgt atgcgtctga ttgccgcccg
      901 caacggaaag atcaatacgc ccggcagggt ctgtgcccgg ccccgaccat tgcccgccgg
      961 agaggttggt ttgcaattgt aagaactgat cgctgataca aaccgcggta tccgggttga
     1021 gcgataagac ctgcggttgt gtaactacca ccatgatcgt atcccgcacg gtgcagtcat
     1081 ttcttcgata tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagcggga
     1201 gcgaaaccaa ccgttgccgg caagtcaata ctgatggaag acagacagag cagcgttgtg
     1261 tcgggcaggt tgatctgagg aagcccctct accaatactt gtgaaatggc attcaccctg
     1321 caaccgttgg catcagtata agcatagccg atctcgtgca ggttgattcc tgcattcatg
     1381 ggagcaaaaa tgccggtggt cgtatccgtt attccagccc cggtaaaagt gccgcccgac
     1441 ggattaacaa gcaacatgcg cggggcgctg tcaatacaga aggtatctgc cggaaaggaa
     1501 acggaagtcg aagccactac cgtgaggaca ataccggccg aggcgctgca agctccattg
     1561 gtgatgctgt attgaatatt gacgggttga tcgggggtga cgattgcagg attaaaaatg
     1621 ccctgaacag catccgtaat gccgttgccc gaccaagtgc caccttcttc gttggccgtc
     1681 agcgtcagag gagaggagcc ggagcacagt ggccccggcg gaggggtaat gctcaggggc
     1741 acattggtct gcacgacgat ttgtacggta tctctgacga agccgcaagc gcccgatgct
     1801 tcgagtattg cggtataggt tccgggcacg ctatacctga ttccgacagg attggcctgc
     1861 gtagaggagg ctggattgcc gccgggaaaa gaccagttat aggtgttgat ggcgccggta
     1921 taactcacca tcggcgtata gttcaggctg tcgcaggcag gaggtacagc tgcgatggag
     1981 gccgttacag cgggtaaaat ttgcacagtt gttgtgctgg aattggggca ttgtgcggaa
     2041 cccgtagagt atgtgtaggt gtatgtattc ggtgccggcc ccatcggatt caaagtatcg
     2101 ttggacatac caggccccgt ccagattccg ccgggcggag tagctcgcag gacaacgggc
     2161 tggccattgg cacacaccga tgccggattg gccggattga aaacaatagg ctccgtactg
     2221 gccaccacca caggtacggt ttgggtctgc cgcaggggct ggtaatttga agggtaatag
     2281 aaccggactg cgtaaaagtg accccgctga aggaggggcc ggtagcggag gccggcgtgc
     2341 cattggtaaa cgtccattga aaattggtga tctctccgct gtggctgaaa ccgaggtttg
     2401 ctgaagaaag ggtgatctga tcgcaggacg gcgttggggc agtgaggctg atgcttggcg
     2461 gctgataaat attgatggta accgtttcaa aatcctctcc gcaggcgttg aaggcgcgtt
     2521 gtcgtacggt gtaggtgcct ggtcgcagga agttcaccac aatgttgttt gtattgagat
     2581 tcatggtggt gtcggcaaag cgccagcggg tagtatcggc gnnnnnnnnn nnnnnngggc
     2641 gtgatgctcc acaccgtgtt actccactgg ttggcttggt cggtgaagga gatggtagtg
     2701 gggccgcagc caaaggcggg attgtgtgta aatgcagcct ccggcggggc caccacccgc
     2761 accggtcggg tttgcgtatc cgagccgcag gtattggtta cggtgaggga tacggtgtag
     2821 ttgccggggg tggcgtaggt atgcgaagga ttttggagtg tggaggtggt accgtcgccg
     2881 aagttccaga ggtaggtggt tgcattgcaa ctcgcattgg aaaacggaac ggacgtattg
     2941 acacatacct caccttgcac gttaaaatta gcgacaggcc tattttttac ggtcagcgtt
     3001 gttgctgcac ttcgacaggt aattccgttg gcacatgatt ttatcccgat aacccaaatg
     3061 gtataattac tttctgccga ttgacacagc agggaatctg gtacgatgta cacatgagtg
     3121 cccggctgat cctgaaaggt aatggtatct acattgccat cgcaccaata gtaaatcagg
     3181 tagtcgaaat caagcgattg gctttcattg atctccaggt gaatggtatc accatcgcaa
     3241 aattcgttat tgccatctac ggatagcgta agattcacgt tgggaaaagc gcattgattc
     3301 tgattgcaca acacgggttg ggggaggcat tggctccagc caaaatggga gaataacgcc
     3361 aaaaacagga gtattaataa agctctcatg gatggtgttt ttattttttt ccgtaccaga
     3421 tattttcata aatcttggaa cgtcggcttt gtgcagcggt ggggcagggc agtttaccat
     3481 aggacacctc gtcttcccgg cccatgaact tacatcccgg actggccgca aagtgtaccg
     3541 ccaccgatac ctctacaatg ctgcccacgc ccaccgtatt ttgtaaccca ctgatattgg
     3601 gcgagaaaga caagccgaga tcgaagcggc tggtgttgcg gttgccggta gtggtcagta
     3661 cgctggtctc cacattgaac gacaaccaat tggtgttcac cccgctcggc ggccgtcgcc
     3721 cgaagtgata atacgcgccg gctgccacca tgcggctaat accggcctgg atgcctgcct
     3781 ccagatatgt cagcccgcct tgctgctgcc acagcaccgt gggctttacg ctgagataag
     3841 tctgagcgct ataatacggc acaaattccc actcggcaaa agccgtatgt cggggcactg
     3901 tagtagtgcc gatgcgcaac accgattcct gatgcccaaa cacattggca tcgcccagcc
     3961 ctaacaaatt gtgcatgctg tacccaatgc tgaaaacgcc gagatgccgg ttggttttgt
     4021 taaacagtgc cttatacgcc acgccaacag acggcatgaa ataccaaaag gaaccggtaa
     4081 aatcgggcgg tacaaagccg gtaggattcg ggtttccgtt gacgtcgaag aagccgtact
     4141 tggggtccaa ctggtcggaa aacgtgaggc ggctgaaatc aatgcggcgt tgcgcccagt
     4201 tgaaccctac accgatgcgc agattgtgaa tagcatctct cacttccaaa gtcggcgtac
     4261 cggccagcat aaatccgccg ctgaaagtac tcagccggcc gtcgccttcc acatcctgta
     4321 cgacgttgag acccaagtcg aaccaactgc agggcatact ttcttcgagc gtagccgcca
     4381 tggtgcggta gggaatgtct ccgcccaaat gccattggga tttgtacaaa gcccgcagtg
     4441 ataaagcgcc ggtttcgccc gtcagcgccg gattaaacag cgagcggttg ttgaagaaat
     4501 gcgtaaagaa agggtcttgg gcaacgaggc tggttgcgca caagagcaat ggaagaagaa
     4561 ggatttttgg cagcatgacg ggaggttttt tttagtagta taagacgatt aatgatcggt
     4621 ttatcagcga atacagtttg cgtactcaaa acgtcggcca agttatcggt ttatcaacat
     4681 cccaatgata gtaccgtact actacccgat tttcatgtgc cgcgaccata atagtagggg
     4741 cgaagtattt gaatgagcgg agggcgctat gcaatacttt tgttgttgtc actccccatc
     4801 aatttttact cctgacaaac aaagtggcat cctccttcat cagccggagg atttccgcgc
     4861 cgccccttcg aacgatagct attgcagtga ctccatatac ctccttgatg acgacttcgc
     4921 ctatttcact gattgcgtct atacctccag tgcctgcggc caatcttctt tcataaacca
     4981 cattgaagac gtcgcctttc tgaacgcttt gtttattgcc aatatccacc aagagcgttt
     5041 tagcctcccg attgtcctgt tctcgtacct ctatcaccca gccatgcaaa ggaaaaaacg
     5101 ttttcaatac ttcttgcagt tctttaccga ttgcttcaac ggcagcattt tcagcagctt
     5161 gcggtgacat aaaagtttgc ccacggttgg aatcaatatt tctgtattca gggtttttca
     5221 acacataagt tgctttaata tcgtatgttt ttgcatccag aatactcaat tcaaccgtca
     5281 gtttgcaaaa ataagatgag gtgcctgtag tcggaaattt aataaaatct atggatcggc
     5341 cccttaatgc ggccgagact acatattcga cattgtgttg tcgaagaatc gcatcctgat
     5401 tatattcttt caaaccaagt aagccctgct caaggtccgc atttgccaga ggctccgaca
     5461 acagcgttac cctgttgctg ttaaacatct gagtgcgcag tttgaaatct accttaccgg
     5521 cagtttctga aaaatcattg cttgcatatc gcacgggagg cattgctacg actgtacggt
     5581 cgaatgtttt tttaggcgtc gcagggtttg gtaccgtcct gtcggggatc aatatatcgt
     5641 tgtttttatt ttctacggta tccgcccatg cggtgtgagc cggcaccgag gtcgaatcag
     5701 aagaatttcg cggttctgtc tcagggtcag aaactctttg cacctctatt ggggcggcct
     5761 tgcggccatt tgcagtattc tgttctgctc tttggggcgg gtattttgtg gtatcctccg
     5821 cgggttggac agtttcgttg gatggatcag agggagatcg gggcagggtt gtttgggttg
     5881 cttgaatcac ttcttcgggg tttttatcca gccatttgta tagcaaaatc atcataacga
     5941 acgcggcagc gaaccatatc atagccccgc gatatttttt ccaccaaggc acaggtggcg
     6001 tttctgtcgg cggtggcaat tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnataa ggccgtcgag ccattgttct attttttcgt ttagcgcctt attattcatc
     6241 acatcacgat aggttctctt ctattcttct tgcaagctcc tcccgacagg gccgtgtttt
     6301 gtttttgacg gtactctcgg ccaaatctgt atgagaagct atttcgtcgg tgcggtaccc
     6361 ccaggctctc atcaacaaga tgctcaggca aggttctccc attttttcca attcattttc
     6421 tacctttttc caacgcagta tcttatcgat accgccactt ggttcgccgc cggtgagttc
     6481 cttaatttca tccaaatcta tcacatcata ggcaatagct gtttttttac gccaagccat
     6541 tcttttcgca atttttacag catacgtttt caagtaattt atctcgcctc gttgcgtatt
     6601 caaaatctgt ttcatcagcg cccatatcgc atcagcagca atttcctctg catcttcctg
     6661 ccccagcccg gtccgctttg cctcatcgcg taaagcactg aaccacgcct tgaacatata
     6721 ttccagtgcg cgttcccggt ggtagccccc tttgcggagg gcggctttta tctggtcgtc
     6781 ggtccaggtc tgcatacagc gtgtaaaatt aaaaatgttt ttcaaaaatg cgccatatca
     6841 ggcatttgca gcgtcgttgt gctctataag acgcccgacg cccgcccagt cgggaaagtg
     6901 ttggtagcaa tacgtctcct gatgctcgat ggagaagaca ttctgcccgg ccttatactc
     6961 tctgaattca cagatggcag ctatctctgc tttttcagcg ggactgaacg cgctgaagtc
     7021 cttgcaaagc tctaaggctt tttcgatgat gatgccgggc atactattga tggttttgag
     7081 tgggcgagtg ttactgctaa gaataaaata aagccccgaa gcgtttcgct gtgtagtatg
     7141 taggagaata cggtagtgat tcgataggat tttagaaagt ttgaagcagt ctttccatgg
     7201 cgatataggt ttttgacaag acaggagtgg cgtctcggtc ccatgtttgt gaattttgtt
     7261 ttatggtttc gatgcaactg ctcaaccctc tgaaaacttc tttagaggca ctatcaaaag
     7321 ctctgttatt gatttctaat atcgcaggaa ggttttggta gtaggaatat ttttctttga
     7381 taaatgcaat aaattcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnntccaga tactccatat cgctcatgat aacttttcga
     7501 ttaaaaataa ggaatgcttc atagtcttgc tttaaatccg ctatttgctt tctcacatga
     7561 atcccatcat ctgcccccgg acacaactgt aagtattttt caaaactcgc aatggctgat
     7621 ttgtaatact cattagcttc ttgcatcaga gcaaaattat aaatgccatg gcaatcttca
     7681 ggatgttgct gcacataagt tttacaacgt tccaaggctg tctcatactc ctccgcataa
     7741 atagtgctta ctcctctgtc aaaaacatcg cgtgaagcag caggaacatc tactaatgca
     7801 ggtgcgggct ttggcacaaa tattttgttg aatttctgaa aagcgcgagt caattcaaac
     7861 tgattttgtg cattcagata tttacccccc gaaatttgag ccagatattg caagtctctt
     7921 tctgcgggcg atccgggatc aataccaaac cccactgcat aatatcttag attagaagaa
     7981 agcaaaggat tgcccgatgt tgaaatattt tgtgtggtct gcccatactg gccaaaagca
     8041 tagacatccg cagggcgaat gggagtacaa gtagcctgtc catcgctgag aataatcaat
     8101 ttggcttgtt tggcgccggt ctgatcaagc cgctgtaata atttctgtcg cgaagactga
     8161 atggcctcaa ataagggcgt tcctccattg gggattggta tgcggttgac ggcgttctct
     8221 actgctgaaa aatctgtggt aaacccattt gtaatcggga aagtgggatc ggatacacat
     8281 gcgccggaaa atcctctgat accaatttct tgtgaaacac cctgatttgt agcattaccc
     8341 atagttcgca gcgtagcttt ggcagcggct ttggcttctt ctattcggga aaccccgctt
     8401 gacatacgct cgttcatact accggaaaga tcgaaaagga atagggtggg gagattctgg
     8461 ggtatctgct tactaaaagc aacttctttt aacatgattg agaatgtatt ggcagagctg
     8521 cctgcagcca cttcaattga aaatttgatt atccgtccct gaaaacggga tatgtcaaaa
     8581 taaacatctg tgaataaatc ctgtgaagtg tctaaagtat ctctaaagga tacttccatt
     8641 tcagcaccgg ctttaagaat aatcgagccc gtatttgagg atgacttttt gagattaatt
     8701 ctaagtgctt ttacatcagg cggcacaaac agccaattat gtatcactga tgatgaccta
     8761 ccggatacag cctcaataac tatgcctgaa ttggatagta tgtccttgac aggattcgca
     8821 ttgacaatct gccaacccgg taaatggcgt attgtattta tgctccctgt tctggaattt
     8881 atatctatct tttcaaattg tctatcaaag ctaccattca taatgcctcg aactcgacgt
     8941 aattgattcg atataatata cattggatcg aagaagtcaa attctgtaga gtatttgtgc
     9001 atattcgggt cgcatagcaa acttgtcaaa ggtcttgttt cacctccaat tctcgaaaat
     9061 ctatagccac ttgctccaca aggagtagaa acacccgtag aaaaagtcct gattgatgtt
     9121 atgtaagact gctgaatttc ggtatgccct tgtaaatgct gagaccaatc acagttgtac
     9181 gttccgtcaa cggcccgtcc attcaaaatc gaattaatat tagtttgaaa gtaggtgtcg
     9241 ttgaatccgc ttccactcca tgaaattacc ccattattag gtttgtcgtt tgaaggacat
     9301 gtaatattta aattaggatg attatcgaag tctgtgtaat cccctaatat tccccagtcg
     9361 tgtgggtcta tattagtaac atggtcaaca gatattatat tcctaaaaga ggggtaggta
     9421 tttttaagcg taataagtcg ctcaatagcc tccgtattta caactgcccc acggctatga
     9481 cctatgaaat gtatctgatg gagcgtaaat tgctgcctaa gcaaacctcg aactaatgag
     9541 gcaaagagca gatcacccgc cgcctctgta aaccctgcgt tatttatatt gctctcagcc
     9601 gcccagtcaa aaagcagtat tacttcgcca tttaagcaag agccttcttc tgttttaaac
     9661 aatccggata tcttatcgta tgtacaaata catcctctgc ccccagtttt ttctaaaata
     9721 gcttttgcca tattatacat ccactcacct cgcattggag caacaccggt taattggtat
     9781 ccatgtgtaa ttacagtagt tccgaactgc tgcccgtgac aataagtatt aatgaataaa
     9841 aaaagaataa accataaaaa aaaatctttc atcgtatgaa attgtaatta ttaaaagatg
     9901 cgtcatataa gaatcagcac ttattttgct cacggaaata cttttgattc ccgacaaaca
     9961 gcaatccccc tcttttgccc ctcacctcac tccctttaca tacttttcaa acattgctgc
    10021 tcctttgtct aagtgttcaa agtcttcgtt tttatagctg gtcaaaatga gttgattgag
    10081 cgctctggca cgcgcttttg atttagggtg cgaagtatat actgtatcga gaaacatctt
    10141 agccaacatg ccgaaacctg cctctccgag tgtattaagc ggatttccat agctaaagtc
    10201 tgcagaagat aacatgctgc gaaaatcgtt atttagtctt gatttaaaat tcccgtcatt
    10261 ggttattttt gccattttgc cccaaaaacc aactgcctcg cgaatgtcat aacctgccaa
    10321 cagagcatat tgcagcccta cacggtcggc ttggctctct tttgcaggtt gagcgccgaa
    10381 aatattggcc aatgcttcgg ggcgcaaagc ggcataagtt tgttttactg cattggccaa
    10441 tagaggagac aggttgctgt tgcgcgtaaa tttcatcaat agcccctctc caatatttgc
    10501 cataattccg gtcaatactg aactttcata tcgctctcgt ccgtgttcat gtgtgacatg
    10561 tgacacctcg tgtccgagta caatggccag ctctgcttcg ttttccatga cgtccagaat
    10621 gcctgtatga acaaatatca tcccattggg gaatgcaaaa gcatttggta tagggttgtc
    10681 aattacataa aaacggaagt tcaatttatt gggatcattg tcgggcaggt ttttctggtg
    10741 ctccggaatc acgcgctctc ccacgctgtt aatataacct tgcaatcgaa tatcattgtg
    10801 taatttatat gagtattgcc caatgcgaat gtttccctgg tgcaggggca atccaacact
    10861 aagccctata tctgtgggcg tgcttaccat cgcaagacct gatgtgttgt agctctgctc
    10921 cacagcattg cgcagtttag catcagtctc aagaagtaag ttcttacaag cccttacttc
    10981 ttcggctata attactccat catcatcatt cagcttccct tttatttcta aaaaatactt
    11041 cccggttttc aataaagggt ctttaaatga agtaaaaacc ttgcctttgc agtcgcaatc
    11101 ggcatttgtt tttccagtaa tgctaacatt ggaggccaat ttgacttttt gcccatccac
    11161 ataggctata tcatcttcta tgagttccag aagcccttct aatttaacga ctccgtcatc
    11221 ttccaaatct gtcttaatta ctgtcgctac ccgctcatta ttaacaactt caaactctat
    11281 atcaacaata gaccctggta tgattctttt tagattgatg ttttttttct gcttgcttaa
    11341 tgctttattt gatgcagaaa caggcactat gatctcatct tcttcgtagt ctatcgtaat
    11401 atggctgtag gggtctgaaa agtctaagac cagagcatct ttgaaaatgc ctttcagtgc
    11461 tttttgaccc caagccgtct caaatgagag aaaaacagca actattacca aagcggaata
    11521 aaaatagatt atctttttca tttttcataa catttatgag tgaataaaat gcttgaaaag
    11581 acggcactgc cnnnnnnnnn nnnnnnnnnn nnnnnncggc aaaggcgctt tgttgcaccg
    11641 tctgaaaaac tactgatctg gcgaattttt taacgctgct accacaacca cccctttaca
    11701 ataatctggg tatcaggctg accattgcca taagctacgc caatgtcgcg aatggcttgt
    11761 atgtatcggc gcatcaaatc aattctgtct ttatactgtg tgcgctccca cgatacattt
    11821 tccttcattt gtgctcttat atctgacagt aatgcggtgc cttctgtgtt gcattttgag
    11881 ccgggaggca gaatggatag gaaggttacc gctttctgag cgccggcagg ggtaggttcg
    11941 gcagcccacg ccgtctttgc tgcttgtaat agggcttggc aacgttgtgc tgctgctgcc
    12001 tcgaacacct gttctgtttt tgctgtggct ttttccgcgc aaggttggct tacatcggaa
    12061 atctggagta acatctccag tgcttcatca gcttgattag ccttggcaag catatctact
    12121 ctgttcatta acagatcaca tcgcgtattg taaaatttga taatctcgtt tcgtccctcg
    12181 gaaacaaaat ttttcatcgc agaattatcc agcctcagac ttctaaaagc gctttggtaa
    12241 gactcgatat cagtagagcc tactccttta accgcggcac tcgttgttga aaaaatggtg
    12301 cgggtattgc gatctgctat gtacaaattg acttctaatt ctattagaat tttagttggc
    12361 gcagttccgg ttactttttt gtctatcaat attggattgg cggccaaaat aaactgcgga
    12421 aagacggcat ctcctgaaaa cccttgcgtg ttggccaact gccttaatcg gcctaacagc
    12481 ataggacgag cctccaaggg caaattatca gaggcgtcag gaactacggg ggtgaggctg
    12541 atgcgagctt gatcgctcac agttcccaaa gtttcctgtg ccaaaacaaa gtttgaataa
    12601 aaagatgctg ccaataatat gaaaaaaacg atctttttca tggcttttat tttgattttg
    12661 gtgattaact taaaagtagt ccgatatgtg attacccttt gtttgctcct ataattacat
    12721 gagcttgccc cagtcctcta gttcttacgg tagcatcaag atggtagggg gcagttttta
    12781 aaaactttcg aagttgctgt gcaaattccc tggcatcaat ggctttaccc tttgcatcgt
    12841 acaccgggat ttttgcaata acgggcatcc ttacatcact catatcaccg atagaatacc
    12901 ggccgttctg gttgccggct tcacgaaagg tattcttatt aaaccaatta tcaagtattt
    12961 cactgaactc ctcatctcca tattctgtgt caaaatcaat cgaagccccc tttgaaagcc
    13021 ttatgacaat tgagacttct ctgccttctt taaataattt ttcaaaataa tcatttagcg
    13081 ttgcactaaa ttccgtcata cgggtataat tgtcttctct aagcatgatc tctatatttg
    13141 cgttggcaga gggttttcca gcgccttcaa ctcctgctat ttggcgattt gtatatgcgt
    13201 caattccctt tagattaaag gtcatcgttt ttataggtcc ggtttgagta aatgcgaaat
    13261 caacatccag aataatatca gctttggctc gtcttttgat tttatcgagt agagactctg
    13321 tcaactcttc tccggattta tcatacatta cggattcctc ggcgtcttca atatccagat
    13381 ttttcaaggt ggcttccata tcaatcaatg gaaatccctg atcgttcata aaattattga
    13441 tcgccgaaat aattgcccgc atgtttctgt cttccagaaa aaggcgttga tagtcaggta
    13501 ctttaatttt tcgcccctga tcgtcaatta cggtcataaa cccgtttttg ttgcagtaaa
    13561 tatcgcttgg caccaccatc agcgagggct ttttggcctg tgcgtaagcc gccacgttta
    13621 tcatcataaa catggcaatc agcagatatg tgggtgatat tttcatgatg tgtatgattt
    13681 aatttgattt ttgatgaatt ttaaaatccg tcattgagac ctctggtttg cttgtcatca
    13741 accatctgtt tctgcagtcg cttgtggtca acaattacgt caatagatac ttttatcagg
    13801 tttcgcacct ttaccctcgt cggcgtctgg tctcctgata ggttcacaaa ttgcaggtag
    13861 gcgccattgt ttttaaaaaa gtcgttgaag tagtccgaat ttcttttgct aatatcgtca
    13921 attattggcc gagacgggca cccagggttt gttgaggctt taatgccttt gaaaagtaat
    13981 tcctgaacag catttttttt cgctgcaat
//
LOCUS       DAORLF010000055        13948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2340_length_13948_cov_2.053264, whole genome shotgun sequence.
ACCESSION   DAORLF010000055 DAORLF010000000
VERSION     DAORLF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2340_length_13948_cov_2.053264"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..2482)
                     /locus_tag="PK198_06775"
     CDS             complement(170..2482)
                     /locus_tag="PK198_06775"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF38472.1"
                     /translation="MLEYAVLTTLYWAAFYGLYMLALRRHTFFRLNRSYLLAALAAGL
                     ILPLLDGIQWPRPETAEAQAFYVAPVVAGFDAINTWEAVITAAPVEKAFPWKTVLLII
                     YLMGAVLGTIRLTTGLGKLWKLWQSGERTRIGGRRVVLTRSPHLPFSFAHTIFLWRNE
                     QWTPEEEADILRHEQAHISDGHTVDVLLLELLGIIFWFSPPLYLYRKALRLVHEYIAD
                     AEVLRTRHRSEYGRLLIRQAVPGLQIAIAHSFHSSLKQRISMMTKTKTKAAARWQYLA
                     ALPLALGLMLLFSQRSAIAAALPELPAITMEIGDTTDVYKVVEEMPRFSGCEEEATVE
                     ARKACADKQMLEFIYKNLDYPTQARDKKIEGTVVVSFIIEKDGSVSGAEIKKEIGGGC
                     GDEVLRVVGLMPRWIPGKQKGETVRVQFMLPVRFKLETAADQKEVIADHSIVYDFEKK
                     ELKTSVKGTPLVIALRDEKSDNKPLVYVNGERLSNGETDLNQINPDNIKEVSVLKGES
                     AIAFAGQEGKNGVILITLYKPGEKPVKVPVKEGDKQLGALSPLSAQNTGEVELFKMVE
                     EMPRFPGCEDNNKSCADKKMLEFIYQNLRYPALARENGVEGTVVVSFIVEKNGKITEP
                     KIVREIGGGCGDEVMRVVNLMPTWIPGKQRGEVVRVQYNLPVRFQLESGEKAGNAANA
                     APKVLPVENFKIFPNPSKGRFTMSFSAPQKTTEIQVLNTQGSAVFSRVLNDFPGTFRE
                     EIDLGKAPKGNYVIVISQGELVHTANFVKQ"
     gene            complement(2627..2995)
                     /locus_tag="PK198_06780"
     CDS             complement(2627..2995)
                     /locus_tag="PK198_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRF38473.1"
                     /translation="MTTLTKAEEEIMQIIWRLERCTVSDVRDSIAQTEGSKPPHSTIS
                     TIVRILEEKGFLGHKAYGRTYEYFPLVQKADYTKRSINKLVSDYFDGSVNRLVSFLVE
                     EEKLNAEELNQLLKQLDDNQ"
     gene            complement(3002..4159)
                     /locus_tag="PK198_06785"
     CDS             complement(3002..4159)
                     /locus_tag="PK198_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38474.1"
                     /translation="MKRWIVGTAMIWMSAALFAQEAGVSQKYAQAQQAMQQFQYEKAL
                     ELLSFCYHAEPTQADYLLQIGYCNTQLGRFKDAKMFYQQVLRLDSAHIGAISALAGIS
                     ERESNYRQALDWYSRLIALDSLNSYYHKKAGLARLRLGDGMSAVFSLREAYLLNDSDL
                     ELMDHLSSLYMELNADSSAEIILRRGLHIDPGNGRLLHNSARLYSKRRNHAAVVGAVE
                     RSRTEGDSSHYYQMVLAVAYLQLDSLEAGIALLEDLEAQNKGTEHVYAYLGTAWRKKG
                     HAERAEFWYNEAITAAISPKTGSYYADMAAIRAEQKDYKKAAELYEKAYSYSNLPKDL
                     FFWAQYNDLYFKDKTAALRLYQRYLSTNDKEYRERVEIRVQQIREQQHFNK"
     gene            4546..5487
                     /locus_tag="PK198_06790"
     CDS             4546..5487
                     /locus_tag="PK198_06790"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRF38475.1"
                     /translation="MAIFEIDKGVVKRVKPIDFKVEKDLQRLVENNLITIFNCKLIAS
                     EFSTGNIHSGRIDTLAISEDSNPVIIEYKKVASSELINQSLYYLHWIKDHKGDFQIAA
                     NKAFGKEVEVDWSDIRVICLAPEYKKYDLHAVQVMGANIELWQYKIYENGILSIDEVY
                     RRTKTVTQQDITESTLKNPVMVEASRKAAITRKTATYTIEQHTEGLSTTMLELFNEIR
                     DFIIELDNSIEETPKKHYIAYKTSQNFVCLQTYKNKITLYLKLSPAEITLMPKQGRDM
                     TNIGHFGTGDFELNIKDSQDFEETKYLVIEALKNIGG"
     gene            5659..6114
                     /locus_tag="PK198_06795"
     CDS             5659..6114
                     /locus_tag="PK198_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF38476.1"
                     /translation="MEQLADITKPSKEEQRVAMESYNALAEILEGLRKDNPEIEIEET
                     REKIRIPLNALKLLAKILKVTSQGKPISIVPIATEMTTQAAADLLGCSRPHLIKLLEE
                     GEIEYTKIGKHRRVKFEDVMAYKKKMKARQQELLIKIMNADEESGLYDT"
     gene            6104..6667
                     /locus_tag="PK198_06800"
     CDS             6104..6667
                     /locus_tag="PK198_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF38477.1"
                     /translation="MIHSIRFTCVLDTNVIIPIEIRDLLFWFAHDDLYTPKWSKHIFD
                     EWEEVMRRKNVSEEEIKKRIGWVKLAFPDAMVENYEVLIEGLTLPDEKDRHVLAAAIK
                     TNANIIVTNNLKDFPEDYLSTFGLSAKNADDFLTETIDLNPKVAVNSFRKLVLNRRNP
                     DLDEYEVLDCLRRNGLNDTANYLHTLI"
     assembly_gap    6768..6786
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6815..>7143)
                     /locus_tag="PK198_06805"
     CDS             complement(6815..>7143)
                     /locus_tag="PK198_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRF38478.1"
                     /translation="VEYERLADHIPPEPHSFVVIMSFGYRSDEAALRALLHLDFGYIG
                     MMGSKAKVKAMFDQLLAEGIAPERLQRIFAPIGLDIKSETAEEIAVSVAAQLIAAKNR
                     PVFRDS"
     assembly_gap    7144..7153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7154..7752)
                     /locus_tag="PK198_06810"
     CDS             complement(<7154..7752)
                     /locus_tag="PK198_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRF38479.1"
                     /translation="MVLAFYTSLTEALRREGRAVLMVVTESTGSSPGRRGFKMWVSPT
                     GMNGTIGGGIMEHKWVEYARSLLSGPAFAPFSKLQIHDKSPGTDRSGLICSGRQTIAF
                     YDVGAGQIPLLESAGQPPGLALTYTEAGIFAEAPFASGAGWQYTDAPAPAHRVYIIGG
                     GHVSRALCEVLSRLDFEIHVFDNREQLNTLMDNPFAHSRT"
     gene            complement(7743..8234)
                     /locus_tag="PK198_06815"
     CDS             complement(7743..8234)
                     /locus_tag="PK198_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38480.1"
                     /translation="MSERWKIILKNHLGGGLIYMCGDAVAALITGEFGMLRALGVFVI
                     GAGLYGFEIPRYFRWIESKMAGVPDKRRAALKTGLALVYFNPLWIARHLCLVYLVSGK
                     TGEIGWQLLRISGLSFLINIPVSIVANYLIQNKVPLRYRFWVSALFSGLMAIYYSMSS
                     AWF"
     gene            complement(8231..>8666)
                     /locus_tag="PK198_06820"
     CDS             complement(8231..>8666)
                     /locus_tag="PK198_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF38481.1"
                     /translation="RGTYTFDSVDVVHDFGNSMNMAVDLGQIEGGLVQGIGWMTLEEV
                     AYNAQGRLLSNALSTYKIPDIYSVPKSIRIASLETEGHDLAILKSKAVGEPPLMYGLG
                     AYFAIREAIRAFNPGADLKVDAPYTPEKVLLDLYRSPSSQGT"
     assembly_gap    8667..8676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8677..>10321)
                     /locus_tag="PK198_06825"
     CDS             complement(<8677..>10321)
                     /locus_tag="PK198_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF38482.1"
                     /translation="DIPGENQIGGILPDEPLFAEGEVHFWGQPVGLIVADSDHICRKA
                     ARLIEAEYEALPVVTDPREAQQLGRLICPPRTFVIGDTAGAWSQCAHIFEGQTAVNGQ
                     EHLYIEAHGAYARMKEDGSVIISSSTQGPTAVQRTVAKVLDLPMHKVEVDTVRLGGGF
                     GGKEDQATGWAVMAALAAQHLQGPVKLVLHRLDDMRMTGKRHPYEADFKIGLDEHLRI
                     IAWEVTHYQNAGAAADLSTAVMERTLFHSTNAYHIPNVRATAYSCKTNLPPNTAFRGF
                     GGPQGKFVIEAAIALAAERIGVSAAEIQQANLLRDGDEMPYGQIMQQTQARSTWAACM
                     ERYNYTGQRERVQAFNEQNTRYKKGMAVQPICFGISFTNTMMNHARALVHIYQDGSVV
                     VSTGAVEMGQGVNTKLVQVAAQSLGISPGRVRIESTNTLRVANTSPTAASAGADLNGK
                     ALQTACAALMERLKKVAEAALGHSDVAIQNEVVMAGGKPSELGWEQLVLMAHQQRVSL
                     SENGHYATPLIHFDKSKEKGHPFAYHVYGTAITIVTVDCL"
     assembly_gap    10322..10393
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10507..>11655)
                     /locus_tag="PK198_06830"
     CDS             complement(10507..>11655)
                     /locus_tag="PK198_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRF38483.1"
                     /translation="KRLNRAQEAMVQHSATQCGFCTPGFVVSLCGYALNVQKADAAGA
                     VGAIDGNICRCTGYKSIERAAGDLALHLEKKDAAHALPWLVSERFLPDYFTGIPERLS
                     QLNNKDRKLHSGHTPIGGGTDLYVQKHHEIPDMELQFMARQKPADAILFDENSCTLTA
                     QATATDLMEAAPLSEAVPGWRQFLKLVSSTPIRNIGTVAGNIANASPIGDLSIMLLAV
                     GARLTLVDESLSTERGIALDGLYLDYKKLDKTPAEIIRRIRFDIPRKGQFFNFEKVCK
                     RQYLDIASVNTALLVTVENEKITTARCSIGGVAPIPKYLRETSRFLEGRSLSAETVRQ
                     ALAVLQTEISPISDVRGTAEYKHLLAKQLFCAHFVELFPQTIKMEDLL"
     assembly_gap    11656..11665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11666..11980)
                     /locus_tag="PK198_06835"
     CDS             complement(<11666..11980)
                     /locus_tag="PK198_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF38484.1"
                     /translation="MHIFVPLRHANRRWFSDMIQFILNQTLIETNKPPGLTLLDFVRY
                     EAHLCGTKIGCREGDCGACTVLIGALHGGEMQYQSATSCLTPLANVHGRHVVTVEGLN
                     LPG"
     gene            complement(12077..>12669)
                     /locus_tag="PK198_06840"
     CDS             complement(12077..>12669)
                     /locus_tag="PK198_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF38485.1"
                     /translation="CVNSKSIDNRVSVFILIEALRALQGKQLPYDVYAVFTVQEEVGI
                     RGAISAAHLIDPDFGLGLDVTIAFDVPGAQPHEMVTRLGKGAAIKILDGSVICDYRMV
                     NFLKKLASDHDIPWQLEILPAGGTDTAGVQRSGKKGAIAGAISIPLRHIHQTIELAHK
                     RDIRNCIDLLTLALEKMDQHHWGFGPEGAGGSEDWM"
     assembly_gap    12670..12686
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12687..12995)
                     /locus_tag="PK198_06845"
     CDS             complement(<12687..12995)
                     /locus_tag="PK198_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HRF38486.1"
                     /translation="MVAAHMDEISFIITHIDEDGFLRFHPLGGFDPKTLTSQRVIVHG
                     RRDIIGVMGSKPIHLMKPEERTKSVPINEYFIDTGMSKAEVAEIVSVGDPVTRERELI
                     E"
     assembly_gap    13005..13131
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13394..13573
                     /locus_tag="PK198_06850"
     CDS             13394..13573
                     /locus_tag="PK198_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38487.1"
                     /translation="MKKVPIPFWEIKLVPRLWRGRGSWLLGKATEYSQYSIYAIYETS
                     ELAINVLAPRIISTL"
     gene            13575..>13948
                     /locus_tag="PK198_06855"
     CDS             13575..>13948
                     /locus_tag="PK198_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRF38488.1"
                     /translation="MSVLKKLAGETAIYGLSSILTRLVNWILLTPYFTRRAFADAPGE
                     YGQINDLYFWIALLLVFTTYRMETAFFRFGRQKEDLHRAFSTASVSLLFTTAAFAVLA
                     SLLLQPIAGALHYEGRPDYILW"
BASE COUNT         3210 a         3541 c         3608 g         3324 t
ORIGIN      
        1 atccaagcct gaaaaccgtg ttggccgacc ctgaaatcag cgtgatcttg ttgaacaaaa
       61 tgtagagcac tcgccctttt ttttgatgag acccttcaat caaccccaac aaaaagagcc
      121 tgccggtgat gtaccaacag gctcctttta ggtcagaaca aaatcctcgt tactgtttca
      181 caaaattggc ggtatgcacc aactcgccct gcgatatcac gatcacatag ttgcctttgg
      241 gcgctttgcc caagtctatc tcctcgcgga aagtgccggg gaaatcattc agaactcgtg
      301 aaaaaacagc gctgccctgt gtgttgagca cctgaatttc agtggtcttc tgcggcgcgc
      361 tgaagctcat ggtaaaacgg cctttgctcg gattggggaa aattttgaaa ttttccaccg
      421 ggagcacttt gggagcagca ttggcagcat tgccggcctt ttccccactt tcgagttgga
      481 agcgcacggg caggttatac tgcacgcgca ccacctcgcc tctttgtttg ccgggaatcc
      541 aggtaggcat gaggtttacc acgcgcatta cttcgtctcc gcagccgccg ccgatttcgc
      601 gcacgatttt cggctcggtg attttaccgt ttttctctac gataaaactc accaccacgg
      661 tgccttccac gccgttttcg cgggccagtg ccgggtagcg caggttttgg tagatgaact
      721 ccagcatttt tttgtcggcg cagcttttgt tgttgtcttc gcagccgggg aagcgcggca
      781 tttcctcaac cattttgaaa agctccactt cgccggtatt ttgtgcagac aggggtgaaa
      841 gggcgcccaa ttgcttgtca ccttctttaa ccggcacttt caccggtttt tcgcccggct
      901 tgtacagcgt gatgaggatg acgccgtttt tgccttcctg tccggcaaaa gcaatggcag
      961 attcgccttt gagcacactg acttctttga tgttgtcggg gttgatttga tttaaatcgg
     1021 tttccccatt ggaaagccgt tcgccattga catagactaa gggcttgttg tcggattttt
     1081 catcccgcaa ggcaatgacc aacggtgtgc ccttcacgga tgttttcaac tcttttttct
     1141 caaaatcata aacgatgctg tgatcggcta tgacttcttt ttggtcggcg gcagtttcca
     1201 gtttgaaccg caccggcagc atgaactgca cccgcaccgt ttcgcctttt tgtttgccgg
     1261 gaatccagcg gggcatcagg cccactaccc gcaatacttc gtcgccgcag cctccgccga
     1321 tttctttctt aatctctgca ccagaaacgg agccgtcttt ctcaataata aaggaaacga
     1381 ccactgtacc ctctattttt ttgtcgcgag cttgggtcgg gtagtctagg tttttataga
     1441 tgaactccag catttgtttg tcggcgcagg ctttgcgggc ttcaaccgtg gcttcctcct
     1501 cgcatcctga gaagcgtggc atttcctcca ccactttgta aacatcggtg gtgtcgccga
     1561 tctccatcgt gatggccggc agttccggca atgccgccgc aatggcgctg cgctgcgaga
     1621 acaggagcat cagccccagt gccagaggca gggctgcgag gtactgccaa cgggcggcag
     1681 ctttggtttt tgttttagtc atcatagaaa ttcgctgttt taaagatgaa tgaaatgaat
     1741 gtgcaatagc aatttgcaag cccggaacgg cctgccgaat gagcagccgc ccgtattcgc
     1801 tgcggtgacg ggtgcgcagg acttccgcgt cggcaatgta ttcgtgtacc aggcggaggg
     1861 ccttgcgata cagatacagc ggagggctga accaaaaaat aatgcccagc agttcgagca
     1921 gcaacacatc caccgtatgc ccgtcgctga tgtgtgcctg ttcgtgccgc aggatgtcgg
     1981 cctcttcttc gggcgtccat tgctcgttgc gccacaggaa gatggtgtgt gcaaaagaaa
     2041 agggcaggtg cgggctacgg gtgagcacca cgcgccgtcc gccgatgcgg gtgcgttcgc
     2101 ccgattgcca aagtttccat agcttgccca atcctgtcgt caggcgtatt gttcctaaga
     2161 cggcgcccat cagatagatg atgagcagta ccgttttcca gggaaatgct ttctccacag
     2221 gcgctgcggt gatgacggcc tcccaggtgt tgatggcgtc gaaaccggcc acgaccggcg
     2281 ccacataaaa tgcctgtgct tctgctgttt ccggcctcgg ccactgtatg ccgtcgagca
     2341 ggggcagtat caaaccggcg gccaaagcag cgaggaggta gctccggttg agccgaaaaa
     2401 aggtgtggcg tctgagggcc aacatgtaca gaccataaaa cgcggcccag tacagggtgg
     2461 tgagaacggc gtattccagc atggcgagtt gtttttgctt ttgtaatcag cctcaagcta
     2521 taagtatagt attcgtagtg aaggggtgac tgcgggctgt gatcttggct gagtcacccc
     2581 ttcactaacc tgtcgaatca acttttacac tgtatcaagc ctcaagctat tgattgtcat
     2641 ccaattgttt cagcagttgg ttcaattcct ctgcgttgag cttttcttct tccacgagga
     2701 aggagacgag ccggttcaca gagccatcga agtagtcgct gaccaatttg ttgatactgc
     2761 gcttggtgta atcggccttc tgcaccaacg gaaaatactc gtaggtgcgg ccatacgctt
     2821 tgtgccccag aaaccccttc tcctctaaga tgcgcacaat ggtagaaatg gtgctatgcg
     2881 gcggcttgct gccctcggtt tgggctatgc tgtcgcgtac atcgctcacg gtgcatcgtt
     2941 ccaatcgcca gatgatctgc atgatttcct cttcggcttt ggtcagtgtt gtcatcgttg
     3001 gttatttgtt gaaatgttgt tgttcccgaa tttgctgcac gcgaatttcc acgcgctcgc
     3061 ggtattcctt gtcgttggtg ctcaggtagc gttggtacaa gcgcagcgct gccgttttgt
     3121 ctttgaaata cagatcgttg tattgcgccc agaagaacag gtctttgggc agattggagt
     3181 aggaatatgc tttctcatac agctcggcgg cttttttgta atccttctgc tcggctcgga
     3241 tggcggccat atcggcgtaa taactgcccg ttttgggcga tatggcggca gtgatggctt
     3301 cgttgtacca aaattcagcc cgttcggcat ggcccttctt tcgccaggcc gttcccagat
     3361 aggcgtacac atgttcagtg cctttattct gggcttccaa atcttccagc agggcaatac
     3421 cagcctccaa actatccaat tgcaaatacg ccacagccag caccatctgg taataatggc
     3481 tgctgtcgcc ttcggttcgg ctgcgttcta cggctcctac gacggctgca tgattgcggc
     3541 gcttgctgta caatcgggcg ctgttgtgca gcaggcgccc attgccgggg tctatgtgca
     3601 agccccggcg caggatgatc tccgcgctgc tgtcggcgtt gagttccatg tacaggctgc
     3661 tcaggtggtc catcagttcc aggtcggaat cattgagcag gtatgcttcg cgcaggctga
     3721 acactgcgct cataccatcg ccgaggcgca ggcgggcgag gccggctttt ttgtggtaat
     3781 aactattgag cgagtccaat gcgatgagcc gggagtacca gtccaatgcc tgccggtaat
     3841 tgctctcgcg ctcgctgatg ccggccaatg cactgatggc gccgatgtgc gctgaatcta
     3901 agcgcaagac ctgctgatag aacattttgg cgtctttgaa gcggcccaat tgcgtattgc
     3961 aataccctat ctggagcagg tagtcggcct gtgtcggttc ggcatggtag caaaaactca
     4021 gcagttcgag cgctttctcg tactggaact gctgcatggc ctgctgtgcc tgcgcatatt
     4081 tttgagatac gccggcctcc tgggcaaaga gtgccgcgct catccatatc attgccgtgc
     4141 cgacgatcca tcgcttcatg tggcaaaggt ataactaaaa aatacgttat acaactaatt
     4201 ttttaatttt ttctcgtttt ttgattttcg caaggagcag agggctgtaa acggtgctgg
     4261 tttgtacccg tccagacctt acaccagcag gaacatatac gctcattacc cgaatcgggg
     4321 ctgaccgggc gccaagccct tttgacaggc tcaacctgtg aaaaagtttt tgtatagcgc
     4381 agttgacttt ctctccaatt tccattagtt ttgcacagcc gaatccggtg tggagtcttg
     4441 tgaaaaaaac tggatgcggg gggcggttgc ggttggagag aaggtagagg ggcggaaggg
     4501 gcgcctacgg caaatggggg tacaacaaat tataaaaagt aaaatatggc aatctttgaa
     4561 attgacaaag gcgtagtcaa gcgggtaaaa ccgattgatt ttaaagtgga gaaggacttg
     4621 caaagacttg tcgaaaataa tctcataact atatttaatt gcaaattgat agcctctgag
     4681 ttttcgacag gaaatatcca ttctggaaga attgatacat tagcaatatc cgaggactcc
     4741 aatccagtaa ttatagaata taaaaaagta gcatcttcag aactcataaa ccaaagtctg
     4801 tattacttgc attggattaa agaccataaa ggagattttc aaattgcagc taacaaagca
     4861 tttggtaaag aagtcgaagt tgattggtca gacattcgtg ttatctgtct cgcccctgaa
     4921 tataaaaaat atgatctgca tgctgtacaa gtaatggggg ccaatattga actttggcaa
     4981 tataaaatat atgaaaatgg tatcctaagt attgatgaag tttaccgtcg gacaaagaca
     5041 gtaacgcaac aagacataac agaaagcacc ctgaaaaacc cagtaatggt tgaggcgagt
     5101 agaaaggctg cgataacaag aaagacagca acctatacaa ttgagcaaca cacagaaggt
     5161 ttaagtacaa cgatgctgga actctttaat gaaattcgag atttcattat cgaattggac
     5221 aattcgattg aagagacacc aaagaaacat tatattgcct ataaaacaag tcagaacttc
     5281 gtgtgcctcc aaacgtacaa aaataaaata accctatatt tgaaactaag cccggcagag
     5341 ataacgctaa tgccaaagca aggaagagat atgacaaaca tcggtcactt tggcacagga
     5401 gattttgaat tgaacataaa ggacagccaa gactttgagg aaactaaata tctggttatt
     5461 gaagccttga aaaatattgg aggataaaaa tgatataaca acggccttgc tgtccatgcc
     5521 gccggacagt cagcccacaa acccaacgca gggcagcaag ccaaccgttc tcatcaatta
     5581 gaaaaaaaaa cgtttcgatt attttgataa tttcgtttat ttcgattatt tttgcagttg
     5641 taatcaatca ttcaagtcat ggaacaatta gcagatatta ccaaaccttc gaaagaagaa
     5701 cagagggtgg caatggaatc ttacaacgcc ctcgcagaaa tccttgaagg ccttcgaaaa
     5761 gacaatcctg aaatagaaat agaagaaaca cgagagaaga ttcggatacc tcttaatgcg
     5821 ttgaaattgc ttgccaaaat cctgaaagtc acaagtcagg gaaagcctat ttcaatagtc
     5881 ccgatagcga ccgaaatgac tacccaagct gctgcggact tgcttgggtg ttcccggcct
     5941 catttgataa agttgcttga agaaggagaa atcgagtaca caaaaattgg aaagcacaga
     6001 agggtaaagt ttgaggatgt aatggcatat aagaaaaaga tgaaagcacg gcagcaggaa
     6061 ctccttatca aaataatgaa cgcggacgaa gaatctggtt tgtatgatac atagcattcg
     6121 atttacatgt gtcctcgaca cgaatgtaat tatccccatc gaaatccgtg atttgctttt
     6181 ttggtttgca cacgatgatt tgtacactcc aaaatggagc aaacacattt ttgatgaatg
     6241 ggaagaagta atgagaagaa aaaatgttag cgaagaagag ataaagaaaa ggataggttg
     6301 ggtaaagttg gcttttccag acgcgatggt tgaaaattat gaagttctga ttgaggggtt
     6361 gacattgccc gatgaaaaag accgtcatgt tcttgcagcg gcaatcaaga ccaatgcgaa
     6421 cattatcgtc actaataatt tgaaagattt cccagaagat tatctctcaa cctttggact
     6481 gtcagctaaa aatgcagatg attttctgac tgaaacaata gacctgaacc caaaagtagc
     6541 agttaactct ttcagaaaac tggtcttaaa cagacggaat cctgatttag acgaatacga
     6601 agttttagat tgcctgagaa gaaacgggct gaacgacacg gcaaattatt tacacacact
     6661 aatttagaaa aaggaaaacc gaagagaatc tcttcattgc ccatcaaata aagaaaacaa
     6721 tcgccgcttc cctgcgcctg ccatctcgaa aaacagccgg catcggcnnn nnnnnnnnnn
     6781 nnnnnnatag cctgatggtg ttgcagtaag aggatcaact gtctcggaaa accggcctgt
     6841 ttttcgccgc tatcaactgc gccgccacgc tgacggctat ctcttctgcc gtttcgcttt
     6901 tgatgtccaa tccgatgggc gcaaaaatgc gttgcaggcg ctccggcgcg atgccctctg
     6961 ccaacaactg atcgaacatg gctttcacct tggccttgct gcccatcatg ccgatgtagc
     7021 cgaaatccag atgcaacaaa gcccgcagtg cggcctcgtc gctgcgatag ccaaacgaca
     7081 tgatgactac aaaactgtgc ggctccggcg gtatgtgatc ggccaatcgc tcgtattcca
     7141 ctannnnnnn nnngtgcggc tatgcgcaaa agggttgtcc atcagggtgt tgagttgctc
     7201 ccggttgtcg aaaacgtgga tttcaaaatc gagccgcgac aacacctcgc acagcgcccg
     7261 actcacatgc cccccgccga tgatgtacac ccggtgtgcc ggagccggcg cgtcggtgta
     7321 ttgccacccg gcgcccgaag caaaaggcgc ttccgcaaaa atgccggcct ccgtataagt
     7381 cagcgccagg cccggcggct gcccggctga ttccaacaac ggaatctggc ccgcgccgac
     7441 atcgtaaaaa gcaatggttt gccgcccgga acagatcaaa ccggagcggt cggtgccggg
     7501 cgatttatcg tgtatctgca acttggaaaa aggcgcaaac gccggccccg acaacagact
     7561 gcgagcgtat tccacccact tgtgttccat gatgccgccg ccgatggtgc cgttcatgcc
     7621 cgttggggaa acccacattt tgaagccgcg cctgcccgga ctgctgccgg tgctctccgt
     7681 cacgaccatc aatacagccc ggccttcgcg gcgcagcgct tccgtcagag aggtgtaaaa
     7741 tgctaaaacc atgcgctgct catcgaataa taaatggcca tcaaaccgga aaacagggcg
     7801 ctgacccaga agcggtatcg caggggtact ttgttctgaa tgaggtagtt ggctacaata
     7861 gacaccggaa tattgatcaa aaacgacagg ccgctgatgc gcaatagctg ccacccgatt
     7921 tcacccgttt tgcccgaaac caaatacact aaacacagat ggcgcgcaat ccacaggggg
     7981 ttgaaataca ccaatgccag acccgttttg agtgccgccc tgcgcttgtc gggcacgccg
     8041 gccatttttg attctatcca gcgaaaatag cggggtatct cgaagccata cagcccggcg
     8101 ccgatgacaa acacgcccaa cgcccgcagc ataccgaact ccccggtaat gagcgccgcc
     8161 accgcatcgc cgcacatata gatgaggccg ccgccgaggt ggtttttcaa aatgatcttc
     8221 cagcgttcgc tcatgtgcct tgcgagcttg gggaacgata caaatccaac agcacttttt
     8281 cgggcgtgta gggcgcgtcc accttcaggt ctgctcccgg attgaaggcg cgaatggcct
     8341 cccgaatggc gaaatatgcg cccaaaccgt acatcagcgg cggctcgccc acagctttcg
     8401 atttgagaat ggccaagtcg tgcccctcgg tttccaagga cgcaatgcgg atggattttg
     8461 gaacagaata gatgtcgggg attttgtagg tagaaagcgc gttcgacaac aggcggccct
     8521 gcgcattgta ggccacctct tccagcgtca tccagccgat gccctgcacc aagccgccct
     8581 caatctgccc cagatcaacc gccatattca tgctgttccc gaaatcgtgt acaacgtcca
     8641 cactgtcaaa ggtataagtg ccgcgcnnnn nnnnnnaggc agtccaccgt gacgatggtg
     8701 atggccgtgc cgtaaacgtg ataggcgaag ggatggccct tttccttgct tttatcaaaa
     8761 tgaataagcg gcgtggcata gtgcccgttt tcgctcagcg ataccctttg ctggtgcgcc
     8821 atcagtacca attgctccca gccaagttcc gagggctttc cgccggccat gaccacctcg
     8881 ttttggatgg caacatcgct gtggccgaga gcggcttcgg ctactttttt cagtctttcc
     8941 atcaaagcgg cgcaggcagt ttggagcgct ttgccgttga ggtcggcgcc cgcgctggct
     9001 gccgtaggcg aggtattggc cacgcgcagg gtattggtgg attcaatgcg cactctgccc
     9061 ggcgagatgc ccagcgattg cgcggccacc tgcaccaact tggtgttcac gccctgcccc
     9121 atttccaccg cgccggtact gaccaccacg ctgccgtcct gatagatgtg taccaatgcg
     9181 cgggcgtggt tcatcatggt gttggtaaac gaaatgccga agcagatggg ctgcacggcc
     9241 atgccttttt tgtaacgggt gttttgttcg ttaaaagcct gcacgcgctc ccgttgtccg
     9301 gtgtaattat agcgttccat acaggctgcc caagtgctgc gggcctgtgt ttgctgcatg
     9361 atttggccgt aaggcatttc gtcgccgtcg cgcagcagat tggcctgttg aatctccgcc
     9421 gcgctgacgc ctatccgttc cgcagccagc gctatggccg cctcgatgac gaacttgccc
     9481 tgcggcccgc cgaacccgcg aaaagccgtg ttgggcggca ggttggtttt gcaactgtag
     9541 gccgtggcgc gcacattggg tatgtggtag gcattggtgg agtgaaacag tgtacgttcc
     9601 atcaccgcgg tggagaggtc ggcggcagcc ccggcatttt gataatgcgt cacttcccag
     9661 gcgatgatgc gcaaatgctc gtccaaacct attttgaaat cggcctcgta gggatgccgt
     9721 ttgccggtca tgcgcatgtc gtccaagcgg tgcagcacca atttgacggg gccttgcagg
     9781 tgttgggcag ccaatgcggc catcaccgcc cagcccgttg cctgatcttc cttgccccca
     9841 aagccgccgc cgagccgcac ggtgtccact tccactttgt gcatgggcag gtctaatact
     9901 ttggccaccg ttcgctgcac cgccgttggt ccctgcgtgg aggaagaaat gatgaccgag
     9961 ccgtcttcct tcatgcgggc atacgcacca tgcgcctcga tgtagaggtg ttcctgcccg
    10021 ttcacagcgg tttgaccttc aaaaatgtgc gcgcattggc tccaggcccc ggcagtatcg
    10081 ccgatgacaa aagtgcgcgg cggacagatg aggcgcccca gttgctgtgc ctctctgggg
    10141 tcggtcacca caggcaatgc ctcgtattcg gcctcgatga gccgggcggc ctttcggcaa
    10201 atatgatcgg agtctgccac gatcaaaccg accggttggc cccaaaaatg tacctcgcct
    10261 tctgcgaaaa gcggctcgtc gggaaggatg ccgccgatct ggttttcgcc gggaatatcg
    10321 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnggcgtgc gcggtcggca ggtcgaaagg cagcgcgtac aaagtgcctt
    10441 gcagttcggg aatgtcgtcg agatagacag agcgaccccg cacatggttt tcagcatcta
    10501 tattgatcat agcaggtctt ccattttgat ggtttgcggg aacaactcca cgaaatgcgc
    10561 acagaataac tgtttggcca acaggtgttt atactcggct gtgccgcgca cgtcgctgat
    10621 gggtgagatt tcggtttgca acacggcaag ggcctgccgg acggtttcgg cagacagtga
    10681 acggccttcc agaaaacggc tcgtctcgcg caggtatttg ggaatcgggg ccacgccgcc
    10741 tatggagcaa cgggcggtgg tgattttttc attttcaacc gtcaccaaga gcgccgtatt
    10801 caccgaggcg atgtccaaat attggcgttt gcacactttt tcaaagttga aaaactgccc
    10861 ctttcgggga atgtcaaacc ggatacggcg gatgatttcg gcaggcgttt tgtccaattt
    10921 tttgtaatcg agatacaagc cgtccaaggc gattccccgc tccgttgaca aactttcatc
    10981 caccaaagtc aggcgcgcgc cgacggccag cagcatgatg ctgaggtcgc cgatggggga
    11041 ggcgttggca atattaccgg ccacggtgcc gatgtttcgg atgggcgtgg aggagactaa
    11101 tttcagaaac tgcctccagc cgggaaccgc ttccgacagt ggcgcggctt ccatcagatc
    11161 ggtggcggtg gcctgtgccg tcagcgtgca gctattttca tcgaacagga tggcatcggc
    11221 aggcttttgg cgcgccatga actgcaattc catgtcggga atttcatggt gcttttgcac
    11281 atacaggtcg gtgccgccgc cgatgggcgt gtggccggaa tgcagtttcc ggtccttgtt
    11341 gttcaattgg ctcaagcgtt cgggaatgcc tgtaaagtaa tcgggcagaa acctttcgga
    11401 aaccaaccag ggcagcgcat gggcggcgtc ttttttttct aagtgcaggg ccagatcacc
    11461 ggcggcccgc tcaatggatt tgtaacccgt gcaacgacag atattgccgt ctatggcgcc
    11521 gacagcgcct gctgcatcgg ccttttggac gttcagcgcg tagccgcaca aagacaccac
    11581 aaagcccggc gtgcaaaatc cgcattgcgt ggccgagtgt tgcaccatcg cttcctgcgc
    11641 ccggttgagc cgcttnnnnn nnnnngccgg gcaggttcag tccctccacg gtcactacat
    11701 gccggccgtg tacattggcc agcggggtga ggcaggaagt ggcgctttgg tactgcattt
    11761 caccgccgtg cagagcgccg atcagcaccg tacacgcgcc gcaatcgccc tcccggcagc
    11821 cgattttggt gccgcagagg tgggcctcgt accgcacaaa gtcgagtaga gtgagtccgg
    11881 gcggcttatt ggtttcaatg agtgtctggt tgaggatgaa ttgtatcata tcagaaaacc
    11941 aacgcctgtt ggcatggcgc aggggaacaa agatatgcaa tgacgggata tttttgtcag
    12001 gatgaaaaag ggatgggcag acgttttatc atacacacat tggtcactgc caaataagtc
    12061 gtgttctacc ttttgatcac atccaatctt cgctgccgcc ggcgccctcc ggcccgaaac
    12121 cccagtggtg ctgatccatt ttttccaaag ccagcgtcag cagatcaata caattgcgga
    12181 tgtcgcgttt gtgggccagt tcgatggttt ggtggatatg gcgcagcggg atggaaatgg
    12241 cccctgcaat ggcgcccttt ttgcccgagc gctgcacccc cgccgtgtcg gtgccgcctg
    12301 cgggcagaat ttccaattgc caaggaatgt cgtggtcgct tgctaatttt ttcaggaaat
    12361 tgaccatgcg ataatcacaa atcaccgagc cgtccaatat tttgatggcg gcgcctttgc
    12421 ctaagcgggt caccatttcg tgcggctgcg cgcccggcac atcaaaggcg atggtgacgt
    12481 cgaggccgag gccgaaatcg ggatcaatga ggtgagccgc cgaaatagca ccccggatgc
    12541 cgacctcctc ctgtacggta aacacggcat acacatcgta aggcaactgc ttgccctgca
    12601 aggcccgcaa cgcttcgatg aggataaaaa ccgacacccg attgtcaata gatttgctgt
    12661 tgacgcactn nnnnnnnnnn nnnnnnttcg atgagttcgc gctcgcgggt caccggatcg
    12721 cctacggaga caatttccgc cacctcggct ttgctcatgc cggtatcaat aaaatattcg
    12781 ttgatgggca ccgacttggt acgttcctcc ggcttcatca ggtgtatggg cttggacccc
    12841 atgacgccga tgatgtcccg gcggccatgc acgatgacgc gctgtgaagt gagtgttttg
    12901 ggatcgaatc cgccgagggg gtgaaagcgc aggaagccgt cttcgtcaat gtgcgtgatg
    12961 atgaacgaaa tttcgtccat gtgggcggcc accataattt ttttnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggggtttc
    13141 gcatatcttt ttcaggagtg cggtgtcaat catagtacga tagcgtgtaa ttttatctca
    13201 taaaaattcg agcggtgaaa ataccactat ttcctatgcc cgtgccgctt tcttcttaaa
    13261 attgcagctt cgtagatact tcggtactat tcagcggcta tctaaaagct acgagctatg
    13321 agtcacgagc tatgagtttg ttttctcgtt caggaattag gttttccatt tggggaaatt
    13381 tcaaaatctc atcttgaaaa aagtccctat tccattttgg gaaatcaagc tcgtaccacg
    13441 cctgtggcgt ggtcgcggct catggctttt ggggaaagcg actgaatatt cacaatactc
    13501 aatttacgcg atttacgaaa catcggaatt ggcaatcaat gtattagcac ctcgaatcat
    13561 cagcaccttg taacatgtct gtactgaaaa aattagccgg agaaaccgcc atttacgggt
    13621 tgagcagcat cctgacgcgc ttggtcaact ggattctgct gacgccttat ttcacccgcc
    13681 gggctttcgc cgatgcgccg ggcgagtacg gacagatcaa cgatctctac ttttggattg
    13741 cgctgttgct ggtgtttacc acctaccgca tggaaacggc attttttcgg tttggccgcc
    13801 aaaaggaaga cctgcatcgg gctttttcca cggcctcggt atcgctgctg ttcactacgg
    13861 cagcctttgc ggtgcttgcc tcgctgttgc tgcaacccat tgccggcgcg ctgcattacg
    13921 aggggcgccc cgactatatt ctgtggtt
//
LOCUS       DAORLF010000056        13899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2353_length_13899_cov_2.122508, whole genome shotgun sequence.
ACCESSION   DAORLF010000056 DAORLF010000000
VERSION     DAORLF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_13899_cov_2.122508"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>371
                     /locus_tag="PK198_06860"
     CDS             59..>371
                     /locus_tag="PK198_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRF38489.1"
                     /translation="MSKDHPKPTDAELAILQVLWQSGPSSVRQVHEYLSDDRDTGYTT
                     TLKLMQIMSEKGLVRRNTDARSHIYEAAIAETDVQQQLLRRFVDTAFRGSAMSLVMQA
                     LG"
     assembly_gap    372..524
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <525..>1486
                     /locus_tag="PK198_06865"
     CDS             <525..>1486
                     /locus_tag="PK198_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRF38490.1"
                     /translation="MLHSLWQAPAIAFTLWLALKSRPAYSARRRYALAYAALALVLLA
                     GIVTFGWYYTPETEGIAQVLANEAPVPAAIAEMAAPDVWERFSFWIDAHLHQMVGLWG
                     LGLAFFLTRLSAGWLYAQNLRLRHAKPASEAVQQWALHIAAHLGLRRKVKVLETALLQ
                     IPATIGWLKPVILLPLGMANQLTPQAVEAILAHELAHIRRHDYLLNLLQNIIESVFYF
                     NPAVWYISAIIRREREHSCDDLAVQVCGSSLIYAKALVQIQELNRPGPRLAMALFRKK
                     GALLLRIQRILQPTIQPTSDIMEKITVTGLLLAAVLAFTVRTTS"
     assembly_gap    1487..1678
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1679..2470
                     /locus_tag="PK198_06870"
     CDS             <1679..2470
                     /locus_tag="PK198_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38491.1"
                     /translation="KYEGMVEKKMSETPPPPPPPPAPGMTPPPPPAPPVPGMKQKSIT
                     IEMDGNGPGKMVERQKNVTVTVGDDGKTIISTEEGNGEKKITRTRITQPEMTIIEGND
                     TIVIFSPDGKEMLRPKIEMDGRSMERQEIQMRVLERNMERQARDMERQAHEMDKNAQE
                     MERSFEFRIDPSKPEWRELREAGGEVIRLLGGGKGTIRSVFERELMNDGLISEPGGYK
                     LELTANAMKVNGKKVPSGMADKYRDIYEEHTGISWGKGGKIVIER"
     gene            complement(2578..4128)
                     /locus_tag="PK198_06875"
     CDS             complement(2578..4128)
                     /locus_tag="PK198_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF38492.1"
                     /translation="MAIRMEKDPVQGPGRPDRGNNNSGSGAAGMLIKALPLLLIFIAK
                     RPKLLIPVLLIGAVWYFFLGGSEMLSGGAGGSDSYSFGATLSEEEYYKAEVFEPLVLG
                     QGNKLPLSVSLAKYAPRRMQQGRQGSCIGWASAYGARTILQAQATGQDPNSLAFSPAY
                     LYNQIALEGCQGAYMQDAMQAMVQNGALPFRQFPYDERTCASEPRSNQIQAGQQYRIR
                     GYNRLTYGERDYRPDIQGIKQHLAQGAPVVIGMMVGGTFMSRMLGQKVWYPSSADYSM
                     QGYSGHAMCVIGYDDNLEGGAFQIMNSWGEEWGDRGIAWVRYRDFERFTKEAYGLHPM
                     GKAGQTTDDARMAVEFGLLDMATQKTIPLKQREDIIFQSASPIRKGDKFKVLIANTVE
                     CYVYVLAEEADGSSSVLFPYTDKHSPYCGITGTRLFPKDYSMTADAVGNKDRIAIVVS
                     KQQLDFKKLNAAINSSRQRSYAEKLKEVLGNQRITNIGFQAGATVAFEAQTQGKNAVG
                     MVVELDKR"
     gene            complement(4204..>5039)
                     /locus_tag="PK198_06880"
     CDS             complement(4204..>5039)
                     /locus_tag="PK198_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF38493.1"
                     /translation="TIVGQALGMAIRGLRPIAEIQYLDYLIYGLAPLTDDVATLRYRS
                     NGQQQAPLIIRSRGHRLEGIWHSGSPMSMIINSLRGMYVIVPRNMTQAAGFYNTMLQS
                     DDPAIIIECLNGYRLKERQPDNLTTFTVPLGVPEVLQEGADITLVTYGSCVRIAQAAI
                     SMLEQQGISVELIDVQTLLPFDLEHRIVESLKKTNRILFLDEDVPGGASAYMMQQVLE
                     VQGGYRYLDSAPATLAAHAHRPPYGTDGDYFSKPNPEDVFETIFRMMHEAEPGRFRGA
                     L"
     assembly_gap    5040..5074
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5075..6179)
                     /locus_tag="PK198_06885"
     CDS             complement(<5075..6179)
                     /locus_tag="PK198_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF38494.1"
                     /translation="MARALGLALASKKYRESDALRSGTGFSNEGNEVCFCTIGDASTS
                     EGVFWETMNAAGVMQVPLAVFVWDDGYGISVPVDFQTTKGNISEVLEGFRYSSEKQQG
                     FDIYTVKGWDYPAMVSQFQRAVARMRETHIPALFHVQELTQPQGHSTSGSHERYKTPD
                     RLQWEREHDCILKMEHWMIESGIAGIEECAAIRTKARKYAQECRRRAWSAYMDPVEVK
                     RRELALLYNQFPQEQAHGILEELRGIVNPSLSELLQNARRLLYATIGEPSEVRAQLQD
                     WIKAQESLGDARYHTQLYSHTPYSALKVPVVPPVFSEQSHVLNGFEILNNYFDLTFAS
                     NPAAFAFGEDVGQIGDVNQGFAGLQKKYGEQRIF"
     assembly_gap    6190..6281
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6282..6638)
                     /locus_tag="PK198_06890"
     CDS             complement(<6282..6638)
                     /locus_tag="PK198_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38495.1"
                     /translation="MTENFLTETASPTEIFNYLSLSKEDVLRDFELCCISREASLLGR
                     KEVLSGKAKFGILGDGKEVPQIALAKAFQKGDFRAGYYRDQTLMFALGVSTVEDFFAQ
                     IYADPDNDPFSGGRQMN"
     assembly_gap    6784..6819
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7044..>7358
                     /locus_tag="PK198_06895"
     CDS             7044..>7358
                     /locus_tag="PK198_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF38496.1"
                     /translation="MKKLLSVLVFVFAVAAVAVAQNAPTAQKAAPVSGPAIKFEKYTV
                     DYGTVEYNSDPFRTFKFTNVGNEPLVIKSARSSCGCTVPDWPKEPIAPGASAELKVRY
                     ATD"
     assembly_gap    7359..7374
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7712..7732
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7733..7953)
                     /locus_tag="PK198_06900"
     CDS             complement(<7733..7953)
                     /locus_tag="PK198_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38497.1"
                     /translation="MEISWLHRAFCFASNSYPSRQKSLHIYSIADTLPAKKSWEDLIV
                     FAKAAVCPWKWIQPAAAPMQMAHSMSDTVA"
     gene            complement(7983..>9520)
                     /locus_tag="PK198_06905"
     CDS             complement(7983..>9520)
                     /locus_tag="PK198_06905"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRF38498.1"
                     /translation="KWVPYLMQGVDDWKTAYEKAGFKNAIMAKRAPTPEEDPEWSLLD
                     ARYSAIIYKPSTIPNASGPSTADPRSGEIVESRINWYHNVMLLLRNWYFVQCAPLDPG
                     AQMMTFDDELMGQLIRFVSSHEVGHTLGLRHNHGSSSTVPVDSLRSKAWVEANGHTPS
                     IMDYARFNYVAQPEDNVGRVGLFPRIGDYDDWAIEWGYRLFPEFSSADAERGHLNKWT
                     IEKLKNKRLWFGTESNPDDPRSQAEQVGDDAMKASEYGIKNLKRIVPNLMTWTKEPNE
                     SYGNLKIMYDQVTGQFNRYNNHVAKYVGGIMETPKMVEESGPVYELVSKAEQRRAVDY
                     LNRNLFATPTWLLDKNILNKTGDKALSVTAGLQNGVLQRLLSARTLGKLVDAEAEAGS
                     NAYRINDLLNDLRNGIWSEVPLNQPISVYRRQLQKTYVEALNAVLNPAPPAAASASGF
                     TFSFGPPVDTDKTDVKAAVRAHIVNLRTQVLTAAGTATDAMTKAHLREMAARMDKALD
                     PKA"
     assembly_gap    9521..9547
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9548..10530)
                     /locus_tag="PK198_06910"
     CDS             complement(<9548..10530)
                     /locus_tag="PK198_06910"
                     /inference="COORDINATES: protein motif:HMM:NF028457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HRF38499.1"
                     /translation="MQSISKTLMLAGIFLLASFSLHAQDDGPPAGGPPAAAKPAAGKG
                     NKPKPYAELITDKAVTKSGMFKVHFLDDKYYFEIPNEMLGREILVVNHIAKAPADMRS
                     GFFGYAGDQIGQNVIRFDKGPNDKIFLRKLSFSEYTPDSTSSMFAAVNNSNIQPIAEA
                     FDVKAYAPESKGSVIDVTDFIGGDNDILFFRSAAKRVSQIGNLQKEKSYIVDVNAFPM
                     NLVINTVKTHARTSSSTPGGPPTPGSSTETFTMEMSNSLVLLPEKPMQPRYYDPRIGY
                     FAVGYTDFDLNPQGIEAVRMIKRWRLEPKDEDMEKYKRGELVEPKKPIVFY"
     gene            complement(10669..>10920)
                     /locus_tag="PK198_06915"
     CDS             complement(10669..>10920)
                     /locus_tag="PK198_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38500.1"
                     /translation="TVLNYVNANFPDHNIEAIAITSLKSNSSIPITPCGFCRQVIMEM
                     EYSQQQKIRIILHKIGGPTYVFQSAPALLPLAFIGEMLQ"
     assembly_gap    10921..10930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10931..11158)
                     /locus_tag="PK198_06920"
     CDS             complement(<10931..11158)
                     /locus_tag="PK198_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRF38501.1"
                     /translation="MKQNSIQIDYIEYESLDAMPADDVALIEKAIEIAQKSYSPYSKF
                     PVGAAVRLDNGEMYAGNNQENVAYPSGTCAER"
     gene            complement(11191..11883)
                     /gene="deoD"
                     /locus_tag="PK198_06925"
     CDS             complement(11191..11883)
                     /gene="deoD"
                     /locus_tag="PK198_06925"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRF38502.1"
                     /translation="MASLHLEAEIGEIAETVLMPGDPLRAKHVAETLLTDAVCYNRVR
                     GMFGFTGYYEGKRVSIQGSGMGMGSAGIYVNELITTYQVKKIIRVGTCGAIQRDLDLG
                     RVVIAMSASGDSDANSHHFKGMHYAATADFDLLYAAVEAAKKLGIPTVQGPIFSTNTF
                     YDTESNRWERHGILGVEMESQILFTLAKRFNVKALSILTVSDNILTGAASGQKEREQS
                     YNDMMRIALEIA"
     gene            complement(12030..>13899)
                     /locus_tag="PK198_06930"
     CDS             complement(12030..>13899)
                     /locus_tag="PK198_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF38503.1"
                     /translation="LMLPWKIAPALAMGNTVVLKPAEFTSLTSLLFAEICSDIGLPAG
                     VVNVITGAGATGAALVKHESVQKIAFTGSTEVGKIIRKATAGSGKKLSLELGGKSPFI
                     VFDDADLDSAVEGLVDAIWFNQGQVCCAGSRLLVQESVAETMYAKIKARMENLIVGNP
                     LDKCIDIGAVVDKVQLETITGLVEQGVAEGNQIWQSTQACPSEGYFFPPTMFTNVSPA
                     AKIAQEEIFGPVLVAMSFRSHKEAVALANNTRYGLAASIWTENINLALDIAPQIKAGS
                     VWINCTNMFDAASGFGGYRESGFGREGGKEGLYEYVKPKHEAQLSDQPVLPAIAKEKK
                     AAGPALPGIDRTAKMYIGGKQARPDGGYSQRILDRSGAYIGEVSAGNRKDIRNAVEAA
                     HAVSSWAGMTGHARAQVLYYIAENLSARKEEFARRLAQMLQHSMAEALAEVEQSIERI
                     YTYAAWADKYDGQAHSTIQRNVTLAMPEALGVMAIVCPDEAPLLGFVSTVLPAIAMGN
                     SVVVIPSETAPLSATDFYQILDTSDVPGGTVNIVTGNKDELAAELAKHYNVDGIWYFG
                     SKEGSRKVEELSTDSMKRSWVSYGKFRNWADPAQGEGEVFLRQATQVKNIWIPYGA"
BASE COUNT         2885 a         3544 c         3758 g         3130 t
ORIGIN      
        1 aatcctttgg cctaaaaaaa atgaacactt tatttttgcc ccacgactaa ggaacgatat
       61 gagtaaagac catcccaagc ctaccgacgc agagttggcc atcctgcaag tgctgtggca
      121 aagcggcccc agttcggtac gacaggtaca tgaatacctg agcgacgacc gcgacacggg
      181 ctataccacc accttgaaac tcatgcagat catgtctgaa aaaggcttgg tgcgccgcaa
      241 taccgatgct cgttctcaca tctacgaagc tgctattgcc gaaaccgatg tgcaacaaca
      301 actgctccgt cgctttgtgg acacggcgtt ccgaggctcg gccatgagct tggtcatgca
      361 ggccttgggg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgctg cactctctgt
      541 ggcaagcgcc ggcgatagcc tttacgctct ggctcgcgct caagtcccga cccgcgtaca
      601 gcgcccgccg ccgatacgcc ctggcctacg cggcgctggc attggtgctg ctggcgggta
      661 tcgtcacatt cgggtggtat tatactcctg aaacagaggg cattgctcag gtattggcaa
      721 atgaagcccc cgttccggca gcaatagcgg agatggctgc acccgacgtt tgggagcgtt
      781 tttctttttg gattgatgcc caccttcacc agatggtagg cttgtggggg ctggggctgg
      841 ctttttttct gacacgactc agcgccggct ggctgtacgc ccaaaacctc agattgcgcc
      901 atgcaaagcc cgcttcggag gccgttcagc agtgggcgtt gcacattgct gctcatctcg
      961 gattgcgccg taaagtaaaa gtacttgaaa ccgcgctgtt gcaaattccc gctaccatcg
     1021 gctggctcaa gcccgtcatt ttactacctc tgggcatggc caaccaactc acgccacagg
     1081 cggtagaagc cattctggcg cacgagctgg cccacatccg ccgccacgac tacctgctga
     1141 acctgctgca aaacatcatc gaatcggtgt tttatttcaa cccggcggtg tggtacatct
     1201 cggccatcat ccgccgcgag cgcgaacaca gttgcgatga cctcgccgtg caggtttgcg
     1261 gcagttcgtt gatatatgcc aaagcattgg tacaaataca ggaattgaac cgcccggggc
     1321 cgcgcctcgc tatggccctg ttcagaaaaa aaggagcgct gctgctccgt attcaacgca
     1381 ttcttcaacc aaccattcaa ccaacatccg acattatgga aaaaatcacc gtcaccggcc
     1441 tgctactggc cgccgtactg gctttcacgg tccgtaccac gagcatnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1681 atacgagggc atggtagaga aaaaaatgag cgaaacacca ccgccccctc ctccaccgcc
     1741 tgcacccggc atgacgccgc cgccgccgcc cgcaccgccc gttcccggca tgaaacaaaa
     1801 atccattacc attgaaatgg acgggaatgg accaggcaag atggtggaaa gacaaaaaaa
     1861 tgtgaccgtc acagtaggcg acgacggcaa aaccatcatc agcaccgagg aaggaaacgg
     1921 ggagaaaaaa atcacccgca ctcgcatcac ccagccagaa atgaccatca ttgaaggaaa
     1981 cgataccatc gtcatcttca gccctgacgg caaagagatg ctgaggccca aaattgaaat
     2041 ggacggccgc agcatggaac ggcaggaaat tcagatgagg gtattggagc gcaacatgga
     2101 acgccaggcc cgtgatatgg aacgccaggc gcatgagatg gacaaaaatg cgcaagaaat
     2161 ggagcgcagt tttgagttca gaatagaccc gtccaaaccg gaatggcgcg aattgaggga
     2221 ggccggcggc gaagtcattc ggcttttggg cggcggcaag ggcaccatcc gcagcgtttt
     2281 tgagcgggaa ttgatgaacg acggcctgat ctccgagccg ggcggctaca agctggaact
     2341 cacggccaat gccatgaaag tgaacggcaa aaaagtaccc tccggcatgg ccgataaata
     2401 ccgcgacatc tacgaggagc acacaggtat ttcctggggt aaaggaggga agattgtgat
     2461 tgaaaggtaa agtagagttt cttttaatct gacgcgaaag gcgggtgggg gagactcatc
     2521 cgcctttcgc atttttcttt aaaaacttcc ccagattaca acccctcagt tcgcccccta
     2581 ccgcttatcc aactccacca ccatgcccac cgcgtttttg ccctgtgttt gcgcctcgaa
     2641 agcgacggta gcgccggcct gaaaaccgat gttggtgatg cgttggttgc ccagcacttc
     2701 cttgagcttt tcggcgtagc tgcgttggcg gctgctgttg atggcggcat tgagcttttt
     2761 gaaatcgagc tgctgcttgc taaccacaat ggcgatgcgg tctttgttgc ccaccgcgtc
     2821 ggcggtcatg gagtaatctt tggggaagag gcgcgtgccg gtgatgccgc agtagggcga
     2881 gtgcttgtcg gtgtagggaa acagcacaga actggagccg tcggcttctt cggccagtac
     2941 atacacatag cactctactg tattggcaat gaggacttta aatttgtccc ccttgcggat
     3001 aggcgatgcg gactggaaga tgatgtcttc gcgttgtttc agcggaatgg ttttctgagt
     3061 ggccatgtcc aacaggccga actctacggc catgcgcgcg tcgtcggtgg tttggccggc
     3121 cttgcccatc gggtgcaggc cgtaggcttc tttggtgaag cgttcaaaat cgcggtagcg
     3181 cacccaggca atgccgcggt cgccccattc ttcgccccaa ctgttcataa tctgaaaagc
     3241 gccgccttcg aggttgtcgt cgtagccgat cacgcacatg gcatggccgc tgtacccctg
     3301 catggagtag tcggctgagg atggatacca gactttctgg cccagcatac ggctcataaa
     3361 agtgccgccc accatcatgc cgatgacgac gggcgcgccc tgcgccaggt gctgtttgat
     3421 gccctgtata tcggggcgat agtcgcgttc gccataagtg aggcggttgt aacctctgat
     3481 gcgatactgt tggcccgcct gaatctgatt gctgcggggt tcggaggcgc aggtgcgttc
     3541 atcgtaggga aattgccgga aaggcagcgc cccattttgg accatggcct gcatggcgtc
     3601 ctgcatgtag gcgccctgac agccttcgag ggcaatctga ttataaaggt atgccggact
     3661 gaatgccagc gagttagggt cttgtccggt ggcctgtgcc tgcaaaatgg tgcgggcgcc
     3721 ataagcgctg gcccatccga tgcaggaacc ctgccgacct tgttgcatcc gacggggagc
     3781 atatttagct aaggaaacgg agaggggcag tttgttgccc tgacccaaaa ccaaaggttc
     3841 aaacacctcg gctttgtagt attcttcttc gctgagagtg gcgccgaaag agtaggaatc
     3901 ggagccgcct gcgccaccgg agagcatctc gctgccgccg aggaaaaagt accacaccgc
     3961 gccgatgagc agtaccggta tgagcagttt tgggcgtttg gctataaaaa taaggagcaa
     4021 gggcaatgcc ttgatgagca tacctgctgc gccgctgccg gaattgttgt tgcctctgtc
     4081 gggccggccc gggccttgca ccgggtcttt ttccattctg atggccatag tacgtttttt
     4141 atgcgcaaag atagcacata gcaacgattt ttggtcacgg ggactaagta caagtgtgcc
     4201 tcgctacaaa gcccctctga atcgtcccgg ttccgcctcg tgcatcatgc ggaaaatggt
     4261 ttcaaaaaca tcctccggat tgggtttgga gaagtaatcg ccgtcggtgc cataaggcgg
     4321 gcgatgggcg tgtgccgcca aagtagcggg cgcagaatcc aggtagcgat atccgccctg
     4381 cacttcgagc acttgctgca tcatgtaggc agaggcgccc cccggaacat cttcgtcgag
     4441 gaagagaatg cggttggttt ttttgagcga ctccacaatg cggtgttcga gatcgaaagg
     4501 cagcagcgtc tgaacgtcaa tgagttctac cgaaatgccc tgctgttcga gcatggaaat
     4561 ggcagcctgc gcaatgcgca cacaagaacc ataagtgacg agggtgatgt cggcgccttc
     4621 ctgtaagact tccggcacgc cgagcggcac ggtgaacgtg gtcaagttgt cgggttggcg
     4681 ttctttgagc cggtagccgt tgaggcattc gatgatgatg gccggatcgt cggactggag
     4741 catcgtattg taaaaaccgg cggcctgggt catgttgcgc ggcacaatga cgtacatgcc
     4801 tctcaatgag ttgataatca tactcatcgg agagccggag tgccatatac cctccaagcg
     4861 gtggcccctg gagcggatga tgagcggcgc ctgctgctga ccattggagc ggtagcgcag
     4921 ggtggctacg tcatcggtga ggggggccag tccatatatg aggtagtcta aatactgaat
     4981 ttcggcaatg gggcgcagac ctcttatggc catgcccagc gcctgcccca caatggtccn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgaaaa ttctctgctc tccgtacttc
     5101 ttttgcaggc cggcaaagcc ctgatttacg tcgccgatct gccctacgtc ctcgccaaat
     5161 gcaaaggccg ccggattgga agcgaaagtg agatcgaagt aattgttgag aatttcaaat
     5221 ccgttgagga catgcgattg ctctgaaaaa acaggcggca ccaccggcac tttgagggcc
     5281 gagtagggcg tatggctgta caactgcgtg tggtagcggg cgtcgccgag gctttcctgc
     5341 gctttgatcc aatcttgcag ttgcgcgcgt acttcagatg gctcgccgat ggtagcgtac
     5401 aggagccggc gggcgttttg caataattca gaaagcgagg ggttgacaat gccgcgcagt
     5461 tcctccaaaa tgccgtgcgc ctgctcttgc ggaaactgat tgtacaaaag ggcgagttcg
     5521 cggcgcttga cctccacggg gtccatatag gcgctccagg cgcggcgacg acattcctgc
     5581 gcgtattttc tggctttggt acggatggcc gcacactctt cgatgccggc aatgccggat
     5641 tcaatcatcc agtgttccat tttcaggata caatcgtgtt cgcgttccca ttgcaggcgg
     5701 tcgggggttt tgtagcgttc gtgcgagccg gaagtggagt gcccctgcgg ctgggttagt
     5761 tcctgaacgt ggaacagcgc gggtatatgt gtttcgcgca ttctggcaac ggccctctga
     5821 aactggctta ccatagcggg atagtcccag cccttgacgg tgtaaatatc gaagccttgt
     5881 tgtttctcgg agctgtaccg aaatccttcg agtacttcgg aaatattgcc tttagttgtt
     5941 tgaaaatcaa cgggtacgga gattccatag ccgtcgtccc agacaaaaac agccaatggc
     6001 acctgcatca cgcctgctgc gttcatggtt tcccaaaaga cgccctcgga ggtacttgca
     6061 tcgccgatgg tgcaaaaaca aacctcgttg ccctcattgg aaaaacctgt gccgctgcgg
     6121 agggcatccg attcgcggta ttttttggaa gctaaagcca ggccgagcgc tctggccatt
     6181 tggccgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttcatttg gcggccgccg
     6301 gagaaggggt cgttgtcggg gtcggcatag atttgtgcaa aaaagtcttc cacggtagaa
     6361 acgccgaggg caaacatgag ggtctgatcg cggtagtagc cggctctgaa atctcctttt
     6421 tggaaggctt tggccaatgc gatttgtggt acttccttcc cgtcgccgag gatgccgaat
     6481 ttggctttgc cgctcagaac ttctttccgg cccagcaaac tggcctctct gctgatgcag
     6541 cacaactcga aatcgcgcag cacatcctct ttagagaggg agaggtaatt aaaaatttcg
     6601 gttggagaag ccgtttcggt taggaaattt tcagtcataa tttcggcttt attcaatgga
     6661 taatacttaa aacatccaaa ttgcaggatg gttttaagaa tataatttca aagactacac
     6721 aaatcgggag cgaaattacg aaataagatt gagaaacgag ccaatacgtt tctgaacctt
     6781 tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tattactgcc tttgaagtcg
     6841 cttgtattcc gcgtcggggc ttcttctttt gaaatacatt gttcgtaagt aattgaaagt
     6901 caatttttta aacagagtgt agccaattgt aaatttggat taaccaaacg ttaacctgcc
     6961 ataccgggta gccaggatgc tacggtgcaa tttttggctt aattttgcat ttagattttg
     7021 aaaccataaa aaaatcaaaa ctgatgaaaa aattactttc tgttctcgtt ttcgtgtttg
     7081 ccgtagcggc tgttgctgtg gctcagaatg cacccaccgc tcaaaaagct gctcctgtaa
     7141 gcggaccggc catcaaattt gaaaagtaca ccgttgatta tggtacggta gaatacaact
     7201 ctgatccgtt ccgtactttc aaatttacca acgtaggcaa cgagcctttg gtcatcaaga
     7261 gcgctcgcag ctcttgcggt tgcaccgttc ctgattggcc gaaagaaccg attgctccgg
     7321 gcgcttctgc cgaactcaaa gttcgctatg ctaccgacnn nnnnnnnnnn nnnncagcaa
     7381 gtccatcacg gtaacaacca acgccgaacc tgccgacgtg atcgtcaaca tcaccggcac
     7441 cgtgaagccg aagcctgctg aagctcccgc cgtaccggaa ggccagaaca atatgttcaa
     7501 aagcaacaac taatctctcc tgaatcgaga ggaaaacccg gggcacgcat cggcgccccg
     7561 ggttttttta tgtcgttttc caacgatcca attgcctcag atgcgaggtg taaaaccagc
     7621 gcttgaacca tccgtatccc tgtcttttca gttcgttgcg aacataggag atcatgtgct
     7681 ctacgctggt aaatttatct acaaatgctc cnnnnnnnnn nnnnnnnnnn nngctacagt
     7741 atctgacatt gagtgtgcca tctgcattgg tgccgccgcc ggctgtatcc atttccatgg
     7801 gcataccgca gctttggcaa aaacgatcaa atcttcccat gacttttttg caggcaatgt
     7861 atccgctatg ctgtaaatat gcaatgattt ttgtctgctc ggatagctgt tggaagcgaa
     7921 acaaaaagcc cgatggagcc aagatatctc catcgggctt tttttgttca ggcagaatca
     7981 gatcaagcct tcggatccag cgccttgtcc atacgagccg ccatttcgcg gaggtgggct
     8041 ttggtcatgg catcggtggc ggtacccgct gcggtaagga cttgcgtgcg caggttcacg
     8101 atgtgcgccc tcacggcagc cttcacgtcg gttttgtcgg tgtccaccgg cgggccaaaa
     8161 ctgaaggtga atcctgaagc ggaagcagcg gcaggcggcg ccgggttgag gacggcattg
     8221 agcgcctcca catacgtttt ttgcaactgt cggcgataca cgctgatggg ttgattgagc
     8281 ggcacttccg accagatgcc gttgcgcagg tcgttcagca ggtcgttgat gcggtaggcg
     8341 ttcgagccgg cttctgcttc ggcgtccacg agtttgccga gggtacgggc gctgaggagg
     8401 cgttgcagca cgccattttg gaggccggcg gtgacgctga gcgctttgtc tccggttttg
     8461 ttgaggatgt ttttgtccaa cagccaggtg ggggtggcaa agaggttgcg attgaggtag
     8521 tccacggctc tgcgctgctc ggctttgctg accaactcat acaccggacc gctttcctcc
     8581 accattttgg gcgtttccat aatgccgccc acatacttgg caacgtggtt gttgtagcgg
     8641 ttgaactgcc cggtgacctg atcgtacatg attttcaggt tgccataaga ttcgttcggt
     8701 tctttggtcc aggtcatcag gttgggaacg atgcgcttga ggtttttgat gccgtattca
     8761 ctggctttca tggcatcgtc gcccacttgt tcggcttgtg aacgcgggtc gtcgggattg
     8821 ctttctgtgc cgaaccacag gcgcttgttc ttgagttttt cgatggtcca tttgttgaga
     8881 tggcctcttt cggcgtcggc gctgctgaac tccgggaaca agcggtagcc ccactctatg
     8941 gcccagtcgt cgtagtcgcc gatgcgcggg aagagaccta cgcggcccac attgtcttcg
     9001 ggctgtgcca catagttgaa ccgggcgtag tccatgatgg agggcgtgtg gccgttggct
     9061 tctacccagg ctttgctgcg gaggctgtcc accggcaccg tagaactgga gccgtgattg
     9121 tggcgcaggc cgagcgtgtg tcccacctca tgcgaggaaa cgaagcggat gagttgcccc
     9181 atgagttcgt cgtcaaacgt catcatctga gcgccgggat cgagcggagc acactgcacg
     9241 aagtaccagt tgcgcagcag gagcatgacg ttgtgatacc agttgatgcg gctttccacg
     9301 atttcgccgc tgcgagggtc ggcagtagat gggccggatg cgttgggaat ggtagaaggc
     9361 ttgtaaataa tggccgaata ccgtgcatca agtaggctcc attcagggtc ttcttcgggc
     9421 gtgggggcgc gtttggccat gatggcgttt ttgaagccgg ctttttcgta tgccgttttc
     9481 cagtcgtcaa cgccctgcat caaataggga acccattttt nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnatg taaaacacga tgggcttttt cggctccacc aattcgcccc gcttgtactt
     9601 ttccatgtct tcatccttgg gttccaagcg ccagcgtttg atcatgcgaa cggcctctat
     9661 gccttgcgga ttgaggtcga aatcggtata acccacagcg aagtacccga tgcgcgggtc
     9721 gtagtagcgc ggctgcatgg gcttttcggg caagagtacg agcgagttgc tcatttccat
     9781 ggtgaaagtt tcggtggaac tgcccggagt aggaggcccg ccgggagtgg aagagctggt
     9841 gcgggcgtgg gttttgacgg tgttgatgac caaattcata ggaaaagcat tcacatccac
     9901 gatgtaagac ttttcttttt gaaggttgcc gatctgactg acgcgcttgg ccgccgagcg
     9961 aaagaagaga atgtcgttgt cgccgccgat gaagtccgtc acgtcaatga cgctgccttt
    10021 gctctccgga gcgtacgcct tcacgtcgaa tgcctcggca atgggctgga tgttcgagtt
    10081 gttcacagcc gcgaacatgg aggaggtgga atcgggcgtg tattccgaaa aggagagctt
    10141 gcgcaggaaa attttatcat tgggaccttt gtcaaaacgg atgacatttt ggcctatctg
    10201 atcgccggca taaccgaaaa aaccactgcg catatcggca ggagctttgg ctatgtggtt
    10261 gactaccagg atttcgcggc ccagcatctc gttgggaatt tcaaagtagt acttgtcgtc
    10321 taagaaatgc accttgaaca tgccgctttt ggtcacggcc ttgtcggtga tcaattctgc
    10381 gtaaggcttg ggtttgttgc ccttgccggc ggcaggcttg gcagcagcag gaggaccgcc
    10441 cgcaggtggc ccgtcgtctt gagcatgaag ggagaaggag gccagcagga agatgccggc
    10501 gagcatgagc gttttggaaa ttgactgcat tgttagtatg atattcgggt gaaaaaaatt
    10561 gttgccgagc aaaagtaggc atttggttgc aaaaggttgc atcaagaata gtgaaggggc
    10621 gacttggctt gcagaatgcc acgatggtca ccccttcact aagccctgtc actgcaacat
    10681 ctcgccgata aaagccagcg gcagcagtgc cggcgccgat tggaagacat acgtcggccc
    10741 tccgattttg tgcaggatga tgcggatttt ctgttgttgg ctgtactcca tttccataat
    10801 gacctggcga caaaatccgc agggcgtgat gggaatggag gaattagatt tcagagatgt
    10861 gatggcgatg gcctcgatgt tgtggtcggg gaaattggcg ttcacatagt tcaaaacagt
    10921 nnnnnnnnnn gcgctcggca caggtccccg acgggtaagc cacgttttcc tgattgttgc
    10981 cggcgtacat ctcgccattg tccaaccgga cggcagcgcc taccgggaat ttggaataag
    11041 gcgagtatga tttttgcgct atctcaatgg ctttttcaat gagcgccaca tcgtcggcgg
    11101 gcatggcgtc caatgattcg tactcgatat agtcaatttg tatgctgttc tgcttcatgt
    11161 tttctgcgaa tgaaaaggtg tgtgaaatgc tcaggctatt tccaatgcga tgcgcatcat
    11221 atcgttgtac gactgctctc gttctttctg gccggatgcc gctcccgtga ggatgttatc
    11281 gctgacggtg agaatggaca atgccttgac attgaatcgc ttggcgagcg tgaacaggat
    11341 ttgcgactcc atttccacgc ccaaaatccc gtggcgttcc cagcggttcg attcggtgtc
    11401 gtaaaacgta ttggtgctga aaatcggccc ctgcacggta ggaataccga gttttttggc
    11461 ggcttccacg gcggcgtaca gcagatcaaa atcggcggta gcggcgtagt gcatcccttt
    11521 gaaatggtga gaattggcgt cgctgtcgcc cgatgcactc attgcaatga ccacccgccc
    11581 caagtcgaga tcgcgctgta tggcgccgca ggttcctacc cggatgattt ttttaacctg
    11641 ataagtggta atgagttcgt tgacgtaaat gccggcgctg cccatcccca tgccggagcc
    11701 ttgaatggaa acgcgcttgc cctcataata ccccgtgaac ccaaacatgc cgcgcacccg
    11761 gttgtaacac acagcgtcgg tgagcaatgt ctctgccaca tgcttggctc gcagcggatc
    11821 gccgggcatc agtaccgttt ctgctatctc gcctatttct gcttctaaat gtaaacttgc
    11881 catgatggtg gtatgaatga tgaatgtctg aatgatgaat tataaatggc gaagggtgca
    11941 agcgggcggg aacggctact ggcctcaagc tgctggctga acgctctacg cttcgcaccg
    12001 cctaccgcct accgcctacc gaagaccgcc taagccccat acggaatcca aatattcttc
    12061 acctgcgtag cctgccgcaa aaacacctcg ccctcgccct gcgcgggatc ggcccagttt
    12121 ctgaatttgc cgtagctcac ccaactgcgc ttcatactgt cggtagaaag ctcctctacc
    12181 ttgcgactac cctctttgct gccaaagtac cagatgccgt ccacgttgta atgcttggcc
    12241 aattcggcgg ccagttcgtc tttgttgccg gtgacgatgt tgaccgtgcc gccgggaacg
    12301 tcggaggtat ctaaaatctg ataaaaatcg gtggcagaca agggcgcagt ctccgacgga
    12361 atcaccacca cgctgttgcc catcgctatg gcgggcagca cggtggagac aaagcccagc
    12421 agcggcgcct catcggggca cacaatggcc atcacgccaa gcgcttcggg catggccagc
    12481 gtcacgttgc gctgtatggt ggagtgcgcc tgcccgtcgt atttgtcggc ccaggcggcg
    12541 taagtataaa tgcgctcgat ggattgctcc acctcagcca gcgcctcggc catgctgtgc
    12601 tgaagcatct gcgccaatcg gcgggcgaac tcctccttgc gggccgatag attctctgcg
    12661 atgtaataca agacctgtgc gcgggcgtgt ccggtcatac cggcccagga actgactgca
    12721 tgggcggcct ccacagcgtt gcggatgtct ttgcgattgc ccgccgacac ttcgccgatg
    12781 taagcgccgc tgcggtcgag gatgcgttgg ctgtagccgc cgtcggggcg ggcctgcttg
    12841 ccgccgatgt acattttggc ggtgcggtca atgccgggaa gcgccgggcc tgctgccttt
    12901 ttttctttgg caatggccgg caatacgggc tgatcgctca gttgagcctc gtgtttgggc
    12961 ttcacatatt cgtacaaacc ctctttgccg ccctctctgc cgaagcccga ctcgcggtag
    13021 ccgccgaagc ccgacgccgc atcgaacatg ttggtgcagt tgatccacac cgagccggct
    13081 ttgatttgcg gcgcgatgtc caaggcgaga ttgatgtttt cggtccaaat gctggcagcg
    13141 aggccatagc gggtgttgtt ggccaaagcc accgcttctt tatggcttct gaaactcatg
    13201 gccacgagta ccggcccgaa gatttcttcc tgtgcaattt tggcggccgg cgacacattg
    13261 gtaaacatcg tcggcgggaa gaagtagccc tccgaggggc aggcctgcgt gctttgccaa
    13321 atctggttgc cctcggccac gccctgctcc accaatccgg tgatggtttc caactgcact
    13381 ttgtccacca ccgcgccgat gtctatacac ttgtccaagg gattgcccac gatgagattt
    13441 tccatgcggg ccttgatctt ggcatacatg gtttcggcca cgctttcctg caccaacaag
    13501 cgcgaaccgg cgcagcacac ctgtccctga ttgaaccaga tggcatccac caaaccctct
    13561 acggcgctgt ccaaatcggc gtcgtcgaaa acgatgaagg gcgactttcc acccagttcc
    13621 aacgagagtt ttttgccgct gcctgccgtt gccttgcgaa tgattttgcc cacctcggta
    13681 gaacctgtaa aggcgatttt ttgcacgctt tcatgtttta ccaatgccgc gccggtagct
    13741 cccgctccgg tgatgacgtt gaccacgccc gccggcagcc cgatgtcgga acaaatttcg
    13801 gcaaagagca gcgaggtcaa agacgtaaat tcggcaggtt tgagtacaac cgtgttgccc
    13861 atagccaatg cgggcgctat tttccagggc agcatgagc
//
LOCUS       DAORLF010000057        13827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2368_length_13827_cov_2.148998, whole genome shotgun sequence.
ACCESSION   DAORLF010000057 DAORLF010000000
VERSION     DAORLF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_13827_cov_2.148998"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..>921)
                     /locus_tag="PK198_06935"
     CDS             complement(342..>921)
                     /locus_tag="PK198_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRF38504.1"
                     /translation="GIPVWARIRSVDEKLILQFLDAGISGVMMPGIRTAEDVRRLVAA
                     VKYPPLGQRGLGPVRAADYLMGRLPQGQYVQWANDQIVILPQIETLKCIENLDEILQV
                     EGVDGFIIGPRDLAMSMGYYDGPANDEVKHTINGIMAKVRAAGKWVGTVAGTAEQANA
                     LTENGANIILNSVQGLIQSAGKAFLNEVRKGK"
     assembly_gap    922..955
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1582..2997
                     /locus_tag="PK198_06940"
     CDS             1582..2997
                     /locus_tag="PK198_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRF38505.1"
                     /translation="MISRFAFSRLTLVLVFALSSFIASAQEADIEAGKTMFRNLCAAC
                     HNKDMKSDLTGPALGGLEDRWAAFPKEDLYSWIRNSQALVKAGHPRAVEVYNQWKQVP
                     MTPFTSLTDPEITNIIAYINCTFTNSCPGAVDMNKTVVEVAPKKKDYTFQLLITALIL
                     GILAIVFAGIIANLNYMSKLQEGQEARRKSLVEILTSRGVVGFVIFALIVLGGYTTVN
                     NAVALGRQQGYAPEQPIKFSHATHAGLHKIDCQYCHDGARRSKHSVIPAANTCMNCHA
                     AVDVGSQYGTAEITKIYASIGWNPSEKQYMENYDKMSEADIEKVYRKWIADVYTRDMK
                     DKDPKMDRLEKTVNEQWRAIKASLTNENKSKIQGPIEWVRIHSLPDHAYFNHAQHVSV
                     GKVACQTCHGPVEAMEVVQQHSPLSMGWCVNCHRQTEVKFADNEYYKSYQRYHEEIKK
                     GERKAVTVEEIGGLECQKCHY"
     gene            3042..>5128
                     /locus_tag="PK198_06945"
     CDS             3042..>5128
                     /locus_tag="PK198_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRF38506.1"
                     /translation="MKKKQQEVWIGVEQLTNDAAYQELVEKEFNDMPIVEQLAQENAP
                     ALQTNRRDFLKYLGFGLGAATVAAGCDIPVRRAIPYVVKPDEIVPGIAVYYASSYVYG
                     GDYCSILVKSREGRPIKIEGNTLSKVTKGATSARAQAAVLSLYDTSRINGPHRIQNGK
                     MQKPLKKSDAGLSWADMDKEVMGKLKPESRVRIVAHTILSPTTKKAIADFTAKFPNTE
                     VVMYDPVSSSAMLQANEKSFGQAVIPSYHFDKAQVIVSFDADFLGTWISPIEFAGQYA
                     QNRKVDPKNPKLSRHIQVENHMTLTGSNADNRIMVKPSEQGAAIVALYNELATLSGKA
                     RVSGPALDPAKVSKIKEAAKELAAARGASLVVSGSNNTGEQVLVNAINDMLGNYGATI
                     SFEAASLQRQGNDAKMQALIKEMGAGAVDALFVMDGANPAYDLPNSDKFVEGMGKVKV
                     KVSLSGVPNETLLACDYAAPANHFLESWGDAEPKRGHYSLIQPVIAPLFNTRQAEISL
                     LTWAASENLNAAAEQPAFEYLKEHWQQAMFPKQNNFSTFQAFWDSTLHDGIFEVAGAG
                     AETKPFSADVNEAAAKVRKPGSSELEISFFETVNMGGGQFASNPWLLEMPDPITRTAW
                     GNYLAIPVSWDGSDFKSFKNLNNREIYGQADIVEVDVKGKKQPITAVRQFGQMQNTLG
                     LAIGYGREVTGEVG"
     assembly_gap    5129..5151
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5152..6332
                     /locus_tag="PK198_06950"
     CDS             <5152..6332
                     /locus_tag="PK198_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF38507.1"
                     /translation="INVYPWLSIDADGNTQYYAVNPTVSDKIGTENDFACVQYHHTMG
                     VTEKKGKLTYKDKRTGEQKDVNLDEKVSSRLKDPFNEHGFQGGITERTIIYKGNLKEL
                     PELTEKIHHKREEAQHLNEQTIYPTQKYVKDIYSQGHWWAMHVDLNACIGCGACQVAC
                     VAENNVPVVGKTEVARHHEMTWLRIDRYYYGDYENPNVVYQPMMCQHCDNAPCENVCP
                     VAATNHSSEGLNQMTYNRCIGTRYCANNCPYKVRRFNWLDYTTADIFVANEYRVNGEE
                     IPFYADNLVRMVLNPDVTVRSRGVIEKCSFCVQRIQEGKLTAKKEGRALRDHDVKTAC
                     QTACPTGAITFGDRNNKESLVAQKMDSPLNYIALEEINVLSSVNYAARINNRNENLDA
                     "
     gene            6381..7805
                     /gene="nrfD"
                     /locus_tag="PK198_06955"
     CDS             6381..7805
                     /gene="nrfD"
                     /locus_tag="PK198_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF38508.1"
                     /translation="MSVVVSPIREPLIEGGKTYHQITEDICSPTEKAPNLAWMIAFGL
                     SSAFLGLYIFTVAWTIWFGIGTWNLNRTIGWGWDITNFVWWVGIGHAGTLISAILLLF
                     RQRWRTGVNRAAEAMTIFAVMCAGQFPLIHMGRLWLGIFIFPYPNTRGPLWVNFNSPL
                     LWDVFAISTYLTVSLLFWYSGLVPDFATLRDRSQGLRRKIYNAVSFGWTGSAKHWQRF
                     ESLALILAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQTL
                     MLIARKVLKLEEYITLQHIDSMNKVILVTGTIVGVAYLTELFISWYSGYIYEQYAFYN
                     RVLGPYWWSYVGMMACNVLSPQVFWSRKIRRSIWWTFVMSIFVNIGMWFERFVIIATT
                     LARDYIPSSWSYYVPTWVEIGIFLGTLGLFFTLFLTFTRVAPVVAVAEVKAILKAGGD
                     QYVGPNAKPHHHHGHDEVSTEAHH"
     gene            7840..8367
                     /locus_tag="PK198_06960"
     CDS             7840..8367
                     /locus_tag="PK198_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRF38509.1"
                     /translation="MKHQKEVIYGLYDDEEILLRAVKKAQADHIEIMDVFTPFPVHGL
                     DKAMGLEESRLHIAGFAYGLTGTLTAFLFMTWVFTRDWPIIFGGKPYWSVPSFIPITF
                     EVTVLFAAIGMVVSFFTVCGMAPGRVNPTLDDRITDDKFCIAFNAAGAEEEERDRLRS
                     FLSGTGASEVNMKTI"
     gene            8391..9269
                     /locus_tag="PK198_06965"
     CDS             8391..9269
                     /locus_tag="PK198_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF38510.1"
                     /translation="MKTIKQIVFVLVIAAVMQACQPAGGEFPGSEYMPDMAHPISYEA
                     NAYDAYKYHTWDEQSVMTRKELTGRMHPVKGTIPRGYAGVYFASGDEAQQAVMDNMHG
                     VGSNNAQAIPVNGKVPYYYGNTPEERLRATAEIIANPFPITKSGLESGKELYNIYCGL
                     CHGEKGDGLGYLVRDADPAKGDPGGKYPAAPANLVNEDFSVASNGRFYHAIMYGLNVM
                     GSYADKLSYEERWQVVHYIRSLQAVKNGAKYDESANTLNPAFGTPMKLVAKKVTAAAA
                     PAPVTDSAAPAGPASH"
     gene            9311..>10287
                     /locus_tag="PK198_06970"
     CDS             9311..>10287
                     /locus_tag="PK198_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38511.1"
                     /translation="MDQFVFDAKKRWVLGGGMILGLLCLIITFFVDDQFHTRFWTNYL
                     HNSVFFTGIAFISLFIIAAFTTALAGWSTLIKRIWEAYSLFMIPGLILMLVIVAGVWG
                     HWHHLYHWADTAAVKEDQVLFGKSGFLNKYWYTFGTLIIVGAWIYFAMQIRKTSVAED
                     SNGPGEDFSYHRRIRTLSVIFLPIAGFSSAAMIWQWVMSLDAHWYSTLFAWYSAASWF
                     LAAMALTILTVIYLKSQGYLPKVTADHLHDLGKYLFGISVFWTYLWFSQYMLIWYANV
                     GEETIYFRERLDNYKPLFFLNLIINFVLPFFVLLRNDTKRKIGTMVFVSV"
     assembly_gap    10288..10319
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10320..10577
                     /locus_tag="PK198_06975"
     CDS             <10320..10577
                     /locus_tag="PK198_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38512.1"
                     /translation="FLMIKPGARITAMEAAAHHAGEAAHHVEHATPFAIGFSFPGILE
                     LGTMIGFLCLFLYIVFTRLTQASLTPKNDPYLEESLHHEVV"
     gene            10658..>11525
                     /locus_tag="PK198_06980"
     CDS             10658..>11525
                     /locus_tag="PK198_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRF38513.1"
                     /translation="MTVLLGLLIVVLLAVVVVQIGKVSELATKIRGEEEMQDIVNKRQ
                     GFWSLIFMVVFLLAVTVSGIYYKDWYLGFGPHAAASEHGGSIDSLFKWTLFATGFVFI
                     ITQILLFVFAYRFSGSRHRKAAFVSHDNKLEMVWTAIPAIVMAFLVIGGLDTWNEVMA
                     DIKPGDDYMEIEAVAEQFGWTIRYPGQDNALGERNYKLISGDNPVGQNWKDQKNIDDF
                     HASEIVLPVGKRIRVRVTSKDVLHNFYLPHFRVKMDAVPGMPTYFVFTPKKTTEEYRN
                     ELSKYAIYQAPAD"
     assembly_gap    11526..11551
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11552..12162
                     /locus_tag="PK198_06985"
     CDS             <11552..12162
                     /locus_tag="PK198_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF38514.1"
                     /translation="QRWETFNYELACAELCGKGHFSMKKIVKIVPEEEYKAWAEKQQS
                     FYFSQVRGTSNDPNSDMWFPQEITERKATFEANLDSARIASSADKKILRFEYVNFETG
                     SAKLTPASQYELGFLVDAMQKYPNMSIEVAGHTDNVGDPAGNQTLSEGRAKSVFDFLV
                     EKGIPASRMRAAGYGDTRPVGDNDTEDGRAKNRRTEFRIITQ"
     gene            12228..>13827
                     /locus_tag="PK198_06990"
     CDS             12228..>13827
                     /locus_tag="PK198_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRF38515.1"
                     /translation="MHEEDVLIQELGYDDHFHDHHHGDKFKSNFITTYIFSMDHKMIA
                     KQFLITGMFWAVVGSLMSLVFRLQLGFPEESLLWLKPLLGKWIVVNNGVGSLAPEFYY
                     ALVTMHGTILVFFVLTAGLSGTFSNLLIPLQIGARDMASPFLNMLSYWFFFTAGVVMF
                     LSLFLSTGPFSGGWTAYPPLSILPQASTGSGAGMTLWALSLVLFVVSVLLGGVNYITT
                     VLNMRTKGMSLWRMPLTIWAFFITAIIGLLSFPVLVSAFLLVIFDRTFGTSFFLSEIY
                     IGGQALEHVGGSPILFQHLFWFLGHPEVYIIILPAMGLVSEVLSVHSRKPIFGYKAMV
                     FSIIAIAFLSFIVWAHHMFMSGVNPFISNFFVVFTLIIAVPSAVKVFNWISTLYKGNV
                     RFNAASLFAIGFVSMFISGGLTGIFLGNSAIDIQLHDTYFVVAHFHIVMGVAAFFGMF
                     AGIYHWFPKMYSRFMNETMGRIHFWGTMVGAYAIFWPMHYTGMAGVPRRYYAFDTFET
                     FRHFTELNQFITIAAIITFLFQVVF"
BASE COUNT         3235 a         3559 c         3512 g         3406 t
ORIGIN      
        1 tttttgccgg aaaaaagtac cagaaaaaaa tttacataat ccatttaatc aactgtaaat
       61 ctttatttta tgcaatcgta tcatcgttct ttttctagat aaaaagaacc aaaaatcacg
      121 ccgcgccgtg gcgcggttct ttccggtttt tctgtcgccc atctcctaaa atctgtaatg
      181 ccggtcccgc gttgctcttg gcctgatcgg ctgcttgaga acgagcttag agggcaacat
      241 tagggaaaaa agatgtgtac aaacagtagc tgcctgccgg cagggaacga caaacgtaga
      301 gggcaaacgt agtgtgaccg gcgtgaggcg ctgacctccg gtcatttccc cttccttacc
      361 tcgttcaaaa acgcttttcc tgcactttga ataagccctt gcacggagtt gaggatgata
      421 tttgcaccat tttcagtgag ggcattggcc tgttctgccg tacctgccac ggttcccacc
      481 cacttgccgg cggcgcgcac cttggccatg atcccgttga tggtatgctt cacttcgtca
      541 ttggcaggac catcgtagta tcccatggac atggccagat cgcgcgggcc gatgatgaac
      601 ccgtccacgc cctccacctg aaggatttcg tccaagttct ctatacattt cagggtttcg
      661 atctgcggca ggatgacgat ctggtcattc gcccattgca catactggcc ttgagggagc
      721 cgccccatga ggtaatcggc agcacgcaca ggacccaggc cgcgctggcc taatggcgga
      781 tacttcacag cagctaccaa gcgcctcaca tcctcggccg tgcggatacc gggcatcatt
      841 accccgctga tgcccgcatc gaggaattgc aggatgagtt tctcgtccac gctgcggatg
      901 cgcgcccata cagggatgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctcg
      961 gtaagcgtga tggccccgtg ttcggcatcc atcatataca gatcgaagcc ggtatagccg
     1021 atgtactcac agacgaccgg atcggtagtg ggcgatagaa tgccatacac tacggcgcct
     1081 tgttgaagtt tttcttttgc tgtgttgttt tgcacgatga tgtaggttta agattcattc
     1141 gttcgttggt ataagcgttg ggcagggtgc attacctcaa cccacaccga tgcgctgccg
     1201 gttgggcaaa tatcaacaat ttccctacaa gtaacgagaa gcagactgga atttatcact
     1261 cgcgccgaga ttccatcttc atgtttaccc cgcagagagg gatgcggact atcatgggtt
     1321 ccacctaagt ttaccacgca tggcgtggct tgtaaaacgc tcatttttag ggacctcttc
     1381 cgtctcaaac atgaaaacat gaacctgttt tcaccactgc gcacatcccg tttttagtcc
     1441 gcccctgttt agaagtgatc taaataattt tcgtttttgt ataaattttg ttaaagcatt
     1501 taacagatta tatatttgcg ccacttttca ggctggagcg gatttcagcc aaaaacgaaa
     1561 gacttacaaa caaccgaaac gatgatatcc agatttgcat tcagccgtct gactttggtt
     1621 ctcgtctttg cgctatcttc ttttatcgct tctgcacaag aagctgatat tgaggcaggt
     1681 aaaacgatgt tcaggaactt gtgtgccgct tgccacaaca aagacatgaa aagcgatctc
     1741 actggtccgg ccctcggcgg cttagaagac cgctgggcag ccttcccgaa agaagacctc
     1801 tacagttgga ttcgcaactc gcaggctttg gtcaaagcag gccatccccg cgccgtagaa
     1861 gtgtataacc agtggaagca ggtgccgatg acgcccttca ccagcctcac cgacccggaa
     1921 attacgaata tcattgcgta catcaactgt acgtttacca attcctgccc gggcgcggta
     1981 gatatgaata agacggtggt agaagttgct cccaagaaga aggactacac cttccagttg
     2041 ctcatcacgg cgctgatact gggcattctg gccatcgtgt ttgccggcat cattgccaac
     2101 ctcaactaca tgtcgaagtt gcaggaaggc caggaagccc gccggaagtc cttggtagaa
     2161 atcctgacga gccgcggcgt agtaggattt gtgatctttg cactcatcgt actcggcgga
     2221 tatacaacgg tgaacaacgc cgtggcgctg ggccgtcagc aaggctacgc gccggagcag
     2281 cccattaagt tctcgcacgc tacgcacgct ggtttgcaca aaattgactg ccagtattgc
     2341 cacgacggcg ctcgccgctc gaagcactcc gttattccgg ctgccaatac ttgtatgaac
     2401 tgccatgccg ctgtggatgt ggggtctcag tacggcacgg ccgagatcac caaaatatac
     2461 gcttccatcg gctggaatcc cagcgaaaag cagtacatgg agaattacga caaaatgagc
     2521 gaggccgata tcgaaaaagt ctatcgcaag tggattgccg atgtttatac ccgcgacatg
     2581 aaagacaagg accccaaaat ggaccgcctg gaaaaaacgg tgaacgagca gtggagagcc
     2641 atcaaggcat cgcttaccaa cgaaaacaag agcaaaattc aaggccccat cgaatgggtg
     2701 cgcatccaca gcctgcccga ccacgcttac ttcaaccatg cacagcacgt ttccgtaggc
     2761 aaagtagcct gccaaacctg ccacggcccg gtagaagcga tggaagtggt gcagcagcat
     2821 tctccgcttt cgatgggctg gtgcgtaaac tgccaccgcc agacggaagt gaaatttgcc
     2881 gacaatgagt attacaaatc ttaccaacgc taccacgagg agatcaagaa aggcgaacgc
     2941 aaggcggtga cggtggaaga aatcggcggt ttggagtgcc aaaagtgcca ctattgatcc
     3001 tgaaatcctt tttctcaagt atctaagaat ctcatcatac aatgaagaaa aaacagcaag
     3061 aggtctggat aggagttgaa caactcacca atgacgctgc ttatcaggaa ctcgtagaga
     3121 aggagttcaa cgatatgccc atcgtagagc agttggctca ggaaaatgct ccggcactgc
     3181 aaaccaatcg ccgcgatttt ctcaagtatc tcggttttgg tttgggcgcc gctacggtag
     3241 ctgccggctg cgatattccg gtacgccgcg ccattccgta tgtcgtcaaa ccggacgaaa
     3301 ttgtgccggg catcgccgtt tattacgcat cttcttatgt gtatggcggc gactactgtt
     3361 ccatcttagt aaaaagccgc gaagggcgtc ctatcaaaat tgaaggcaac accctttcca
     3421 aagtaaccaa aggcgccacc agcgcccgcg ctcaggccgc tgtgctcagt ctgtacgaca
     3481 ccagccgcat caacgggccg caccgcatcc agaacggcaa aatgcagaag ccgctcaaaa
     3541 aatcggatgc cggtctttcc tgggcggata tggacaagga ggtcatgggc aaactcaagc
     3601 ccgaatcccg cgtccgcatc gtagcgcata ccatcctcag cccgactaca aaaaaggcca
     3661 ttgccgactt cacggccaaa tttccgaaca ccgaggtagt catgtacgat ccggtgtctt
     3721 cttcggccat gttgcaggcc aacgaaaaga gcttcggcca ggcggtcatt ccgtcttatc
     3781 acttcgataa ggcacaggtg attgtctctt ttgacgccga cttcctgggt acctggattt
     3841 ctccgataga atttgcaggc caatacgccc agaatcgcaa ggtggacccg aaaaatccga
     3901 agctttcccg ccacattcag gtggaaaacc acatgacgct cacgggttcc aatgcagaca
     3961 accgcatcat ggtgaaaccc tccgagcagg gcgcggccat cgtagcattg tacaatgaac
     4021 tcgccacgct cagcggcaag gcgcgcgtca gcggtccggc tcttgatccg gccaaagttt
     4081 ccaaaatcaa agaagccgcg aaagaactcg ctgccgcccg cggtgcgagt ttggtagtga
     4141 gcggctccaa caataccggc gaacaggtgc tcgtcaacgc catcaacgat atgttgggca
     4201 actacggcgc taccatcagc tttgaagccg cttctcttca gcgccagggc aacgacgcca
     4261 aaatgcaggc actgattaag gagatgggcg ccggagcagt agatgccctc tttgttatgg
     4321 acggcgccaa tcccgcttat gacctgccca actccgacaa gtttgtggaa ggtatgggca
     4381 aagtgaaagt caaagtttcg ctttcaggcg tacccaatga aacgctgttg gcttgcgact
     4441 atgcggctcc cgccaaccac ttcctcgaaa gctggggcga tgcagaaccc aagcgcggcc
     4501 attacagcct gattcagccg gtcatcgcgc cgctgttcaa cacccgtcag gcggaaatca
     4561 gcctgctgac ctgggccgct tccgaaaacc tgaacgccgc cgccgaacaa cccgcctttg
     4621 aatacctcaa ggagcactgg cagcaggcca tgttcccgaa acaaaacaac ttctctacct
     4681 tccaggcatt ctgggacagc accctgcacg acggcatctt cgaagtagcc ggcgccggcg
     4741 ctgaaaccaa gcctttctct gccgatgtga acgaggctgc cgccaaagtg cgcaagccgg
     4801 gttcatcgga attggagata tccttctttg aaaccgtcaa catgggtggc ggacagtttg
     4861 ccagcaatcc ctggctactg gaaatgcccg atcccatcac gcgtaccgca tggggcaact
     4921 atctggccat tccggtaagc tgggacggca gtgatttcaa gtcgttcaaa aacctgaaca
     4981 accgcgagat ttacggtcag gccgacattg tggaagtgga tgtgaaaggc aaaaagcagc
     5041 ccatcaccgc cgttcgccag tttggtcaga tgcaaaatac cttgggcttg gccatcggct
     5101 acggccgcga agtgaccggc gaggtaggnn nnnnnnnnnn nnnnnnnnnn nggatcaacg
     5161 tttacccctg gctcagcatt gatgccgacg gcaatacgca atactatgct gtgaatccaa
     5221 cggtgtctga taagatcggt accgaaaacg actttgcctg cgtgcagtac caccacacga
     5281 tgggggtaac tgagaaaaaa ggcaagttga cctataaaga caagcgcacc ggcgagcaga
     5341 aggatgtgaa tttggatgag aaggtatctt cccgcctgaa ggaccccttc aacgagcacg
     5401 gtttccaggg cggcatcacc gagcgtacca ttatttataa aggcaatctg aaagagcttc
     5461 ccgaactgac ggagaaaatc caccacaagc gggaggaagc ccagcacctc aacgagcaga
     5521 ccatctatcc tacacagaaa tatgtgaagg acatctactc gcaggggcac tggtgggcca
     5581 tgcacgttga cctgaatgcc tgcattggtt gcggtgcttg tcaggtagcc tgcgtagctg
     5641 agaacaacgt gccggtggta ggcaaaacag aagtagcccg tcaccacgaa atgacctggc
     5701 tccgcataga ccgctactac tatggcgatt atgaaaatcc caatgtggtt tatcagccga
     5761 tgatgtgcca gcattgcgac aacgcgcctt gcgaaaacgt gtgtccggtg gccgctacca
     5821 accacagcag cgaaggtttg aaccagatga cctacaaccg ctgtatcggt acgcgctact
     5881 gcgccaacaa ctgcccgtac aaagtgcgcc gtttcaactg gttagattac actacggcag
     5941 atatttttgt ggccaacgaa tatcgcgtca atggggaaga aattccattc tatgcggaca
     6001 acctcgtgcg catggtgctc aatccggatg tgacggttcg ctctcgtggc gtgatcgaaa
     6061 agtgcagctt ctgtgtgcag cgcatccagg agggtaagct caccgccaag aaagaaggcc
     6121 gcgccctccg agaccacgat gtgaaaacgg cttgccagac cgcctgcccg acgggcgcca
     6181 ttaccttcgg cgaccgcaac aacaaagaga gtctggtagc gcaaaagatg gacagcccgt
     6241 tgaattacat cgcgcttgaa gaaatcaacg tgttgtcttc cgtcaactat gcggcgcgca
     6301 tcaacaaccg caatgaaaat ttggacgcct aattgatcaa tccggtacgc ccggcgtact
     6361 tctttaaaat agagaacaaa atgagcgttg tagtatctcc aattcgggaa cctctgatag
     6421 aaggcggtaa aacctaccat cagatcaccg aagatatttg ctctcccacc gagaaagcac
     6481 ctaatctggc ctggatgatt gccttcggcc tgagttcagc ttttctgggg ctgtacattt
     6541 ttaccgtagc ctggacgatc tggttcggta tcggcacctg gaacctcaac cgcaccatcg
     6601 gctggggctg ggacatcacc aacttcgtgt ggtgggttgg tatcggtcac gccggtacgc
     6661 ttatctcggc catcttgttg ctgttccggc agcgttggcg cacaggggtg aaccgggctg
     6721 ctgaagccat gaccatcttt gcggtgatgt gtgcgggtca gtttccgctc attcacatgg
     6781 gccgtctttg gctgggcatc ttcatctttc cgtatcccaa cacgcgcggc cctttgtggg
     6841 taaacttcaa ctctccgctg ttgtgggacg tgttcgccat ctccacctat cttacggtat
     6901 ccttgctgtt ctggtattcg ggcttagtac ctgacttcgc tactttgcgc gaccgttcgc
     6961 agggcttgcg ccgtaagatt tacaatgcag tctctttcgg ctggaccggt tctgccaagc
     7021 actggcagcg ttttgaaagc ctcgcgttga ttctcgccgg tttgtccacg ccgctcgtat
     7081 tgtcggtaca caccatcgta tccttcgact ttgccacctc ggtgattccc ggttggcaca
     7141 ccaccatctt ccctccgtat ttcgtagcgg gcgccatctt ctccggcttt gcgatggtac
     7201 aaacgctgat gctcattgcg cgcaaggtgc tgaaattgga agagtacatc actttgcagc
     7261 atatagacag tatgaacaag gtgatcttgg tgacgggtac cattgtgggc gtagcttacc
     7321 tgacggagct ttttatctcc tggtactccg gctatattta cgagcaatac gctttttaca
     7381 atcgcgtact cggcccttac tggtggtcgt atgtaggcat gatggcgtgt aacgtgttgt
     7441 ctcctcaggt gttctggagc cgcaaaatcc gccgcagtat ctggtggacg ttcgtgatgt
     7501 ctatattcgt caatatcggt atgtggtttg agcgctttgt gattatcgcc accacgctgg
     7561 cccgcgatta catcccctct tcctggagct actatgtgcc tacttgggta gaaatcggta
     7621 ttttcctcgg cactttgggc ttgttcttca ccctgttcct cacgttcacc cgcgtagcgc
     7681 cggtggtagc agtagcagaa gtaaaagcca ttctcaaagc cggtggcgac caatatgtgg
     7741 ggccgaatgc caagccgcac caccaccacg gccacgatga agtaagcacc gaagcacatc
     7801 actaatatca actttgaaca agctacaacc gagcatacaa tgaaacatca aaaagaggta
     7861 atttacggtt tgtacgatga tgaggaaatc ctcctccgcg ccgtgaaaaa agcacaggcc
     7921 gaccacatcg aaatcatgga tgtgttcacc ccgtttccgg tacacggctt agacaaagct
     7981 atgggattgg aggagagccg tttgcacatc gctggttttg cttatggcct gacgggcacg
     8041 ctcacggcgt tcttgtttat gacctgggtg ttcacccgcg actggcccat cattttcggc
     8101 ggtaaaccgt attggtcggt gccttccttt attcccatca cttttgaggt gacggtattg
     8161 ttcgccgcta tcggcatggt ggtctctttc ttcaccgtgt gcggtatggc gcccggccgg
     8221 gtcaatccca ctttggacga ccgcattacc gacgataagt tttgcattgc tttcaatgcg
     8281 gcaggagcgg aagaggaaga gcgcgaccgc ctgcgcagtt tcctgagtgg aaccggagct
     8341 tctgaagtaa acatgaagac gatttaattc gccataaaaa gagagcttag atgaaaacca
     8401 ttaagcaaat agtttttgtg ctggtcattg ccgctgtgat gcaggcctgc caacctgccg
     8461 gcggcgagtt tcccggcagc gaatacatgc ccgacatggc gcacccgata tcttatgagg
     8521 ccaatgctta cgatgcctat aagtatcata catgggacga gcaaagtgtg atgacccgca
     8581 aggaactcac aggcaggatg caccctgtga aaggaacgat tccgcgcggt tatgccggcg
     8641 tctatttcgc ttccggcgat gaagctcaac aagcggtgat ggacaacatg cacggcgtag
     8701 ggagcaacaa cgcccaagct attccggtaa acggcaaagt gccctactac tacggcaata
     8761 cgccggagga acgcctgcgc gctacggcag agatcattgc caatcctttc ccgatcacga
     8821 aatcgggttt ggagagcggc aaagagttgt acaatatata ctgtggtctt tgccacggag
     8881 agaagggcga cggcttgggc tatctggtgc gcgatgccga ccctgccaaa ggtgatccgg
     8941 gaggaaagta tccggctgct cctgccaact tggtgaacga agacttttcg gtggcatcca
     9001 acggccgttt ttaccacgcc attatgtacg gtttgaatgt gatgggatcg tatgccgata
     9061 aactctctta tgaagagcgc tggcaggtgg ttcactacat tcgctctttg caggctgtga
     9121 aaaacggcgc caagtatgat gaaagcgcca acacgctgaa tccggctttc ggtactccga
     9181 tgaaattggt tgccaaaaaa gttacggctg ctgctgcgcc ggctcctgta acagatagcg
     9241 ctgcaccggc aggtccggcg agccattgat gtaaatttta ttcaaaagac actttaatac
     9301 gattcgaaca atggatcaat tcgtattcga cgcaaaaaaa cgatgggtat tgggcggtgg
     9361 tatgatactg ggcctgctgt gcctcatcat caccttcttt gtggatgacc agtttcacac
     9421 ccgtttctgg accaactacc tgcacaattc ggtattcttc acggggatcg cattcatatc
     9481 cttgtttatc attgctgctt ttactacggc tttggccggg tggagtacac tgatcaagcg
     9541 gatttgggaa gcctactcgc tgtttatgat tcccggcctc atccttatgc tggtcattgt
     9601 ggccggcgtg tgggggcact ggcatcattt gtatcattgg gccgacacgg cagccgtgaa
     9661 ggaagatcag gttttgtttg gaaaatcggg cttcctcaat aagtattggt acaccttcgg
     9721 cacgctcatc attgtgggcg cctggattta ctttgcgatg caaattcgca agacctctgt
     9781 ggccgaagac agcaatggcc ccggcgaaga tttcagctat caccgccgga ttcgcaccct
     9841 ttcggtcatt ttccttccga tagcgggctt ttccagcgcg gccatgatct ggcagtgggt
     9901 gatgtccttg gatgcacact ggtacagcac gttgtttgca tggtactctg cggccagttg
     9961 gtttttggca gctatggccc tgacgattct cacggtcatt tatctaaaaa gccagggcta
    10021 cctgcccaaa gtcactgccg atcacctgca cgatttgggc aagtacctct ttggtatcag
    10081 cgtattctgg acctacctgt ggttctctca gtatatgttg atctggtatg ccaacgtggg
    10141 tgaagaaacg atttatttcc gcgagcgttt ggacaactac aagccgttgt tcttcctcaa
    10201 cctgatcatc aattttgtgc tgcccttctt tgtgcttttg cgcaacgata ccaagcgcaa
    10261 aatcggtacg atggtctttg tttctgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    10321 tcctgatgat taaaccgggc gcccgcatca cggcaatgga agctgctgca caccacgccg
    10381 gcgaggccgc acatcatgtt gagcatgcca cgccttttgc catcgggttt tcattccccg
    10441 gcatcttgga attgggtacc atgatcggct ttttgtgctt gttcctgtac atcgttttca
    10501 cgcgattgac acaggcttcg ctcacgccga aaaatgatcc ttatctggaa gaaagtctgc
    10561 atcacgaagt ggtgtaaagt ataaacaatt tgcctgttgc ggtatttaag ccacaggaag
    10621 atttcaatta gttttacaac attcaatttt caaaataatg acggtcttac tcggtcttct
    10681 catcgtagtt ctccttgccg tagtagtggt acagatcggt aaagtttccg aattggctac
    10741 caaaatccgg ggcgaagaag aaatgcagga tattgtgaat aagcgtcagg gcttctggtc
    10801 gctcattttc atggttgtgt ttctgttggc ggttacggtt tccggcatct attacaaaga
    10861 ttggtatctg ggctttgggc cgcatgcggc agcatctgag catggcggtt caatagacag
    10921 cctgtttaaa tggacgctat ttgctacagg cttcgtgttc atcatcactc agattttgct
    10981 gttcgtgttc gcttatcgct tcagcggcag ccgccatcgg aaagcagcct ttgtttctca
    11041 cgacaataag ctggagatgg tatggacggc gatcccggct attgtaatgg ccttcttggt
    11101 gattggcggc ttggatactt ggaatgaagt gatggccgac atcaagccag gcgacgacta
    11161 catggaaatt gaggcggtgg ctgagcagtt tggctggacc atccgctatc cggggcaaga
    11221 caacgcactg ggcgagcgca attacaaatt gatttccggc gacaacccgg tgggccaaaa
    11281 ttggaaagac cagaaaaata ttgacgactt ccacgccagc gaaatcgtac tgccggtagg
    11341 caaaagaatc cgggtgcgcg ttacttctaa ggacgtattg cacaatttct acctgcctca
    11401 cttccgtgta aaaatggatg ctgtgccggg tatgcctact tattttgtat tcacgcccaa
    11461 gaaaacgacg gaagaataca gaaacgaact cagcaaatac gctatttatc aggctcctgc
    11521 cgatcnnnnn nnnnnnnnnn nnnnnnnnnn nagcaacgct gggaaacgtt taactacgaa
    11581 ttggcttgcg ccgaactttg cggcaaggga cacttcagta tgaagaaaat tgtgaaaatc
    11641 gttccggaag aggaatacaa agcttgggca gaaaagcagc aatcgttcta cttcagtcag
    11701 gttcggggaa cttccaatga cccgaactcc gatatgtggt tcccgcagga aatcacagag
    11761 cgcaaggcta ctttcgaggc caatctggat tcggcccgca ttgcctcgag cgcagacaaa
    11821 aagatattgc gctttgaata tgtgaacttc gagaccggtt cggccaaatt gacgccggct
    11881 tcccaatatg aattgggctt cttggtggat gccatgcaga agtaccccaa tatgagcatc
    11941 gaagtggcag gtcacacaga caacgtgggt gatccggctg gtaaccagac cttgtctgaa
    12001 ggccgtgcta aatccgtttt tgactttttg gtagaaaaag gaattcctgc ttccagaatg
    12061 cgtgctgccg gttatggcga tactcgcccg gtaggcgaca acgacacaga agacggtcgc
    12121 gctaaaaacc gccgcacgga attccgaatc attacgcaat aatttaacat ccagaccaat
    12181 acataaatct ttagcaaatg gctacaagta ccgtatcaaa ctcaagcatg cacgaagaag
    12241 atgtgctgat tcaggaattg ggctacgatg accacttcca tgatcaccat cacggagata
    12301 agttcaagtc gaatttcatt acgacttaca tcttcagcat ggatcacaag atgattgcca
    12361 agcagttcct gatcacaggt atgttttggg cggttgtcgg cagcttgatg tctttggtgt
    12421 ttcgtctcca gttgggtttc cctgaagaga gcctgctgtg gttgaagcct ttactaggca
    12481 aatggattgt ggtaaacaat ggcgtcggct cattggctcc tgagttttac tatgcgctgg
    12541 ttaccatgca cggcaccata ttggtgttct ttgtattgac ggcaggtttg agcggaactt
    12601 tcagtaactt gctgattccg ttgcagatag gtgcacgcga tatggcttcg ccgtttctca
    12661 atatgttgtc gtattggttc ttttttacgg caggtgtagt gatgtttttg tcgctgtttc
    12721 tcagcaccgg tcctttctcc ggcggctgga cggcctatcc gcccttgagt attctgccgc
    12781 aggcatctac cggttccggc gcgggtatga ctttgtgggc attgagcttg gtactttttg
    12841 tggtgtcggt gttgttgggc ggggtgaatt atatcaccac tgtactcaat atgcgtacta
    12901 agggaatgag cttgtggcgt atgccgctca ctatttgggc gtttttcatc accgccatta
    12961 ttggcttgct ttctttcccg gtattggttt ctgctttctt gttggtcatt tttgaccgca
    13021 ctttcggtac gagcttcttc ctgagtgaaa tctacattgg cggtcaggca ttggaacatg
    13081 tgggtggcag cccgatcttg ttccagcact tgttctggtt cctggggcac ccggaggtat
    13141 atatcatcat tttgcctgcg atgggcttgg tatcggaagt actttcggtg cattcgcgca
    13201 agccgatatt tggttacaaa gcgatggtgt tttctattat cgccattgcc ttcctttcgt
    13261 tcattgtatg ggcgcaccac atgtttatgt caggcgttaa cccattcatt tccaacttct
    13321 tcgtggtgtt tacgctcatc attgccgtac cttcggcagt gaaggtgttc aactggattt
    13381 ccactttgta caaaggcaat gtccgcttca atgcggccag tttgtttgcc atcggctttg
    13441 tttcgatgtt catatccggc ggtttgacgg gtatctttct cggcaactcc gctattgaca
    13501 tccagttgca cgacacatac tttgtagttg cccacttcca tattgtgatg ggggtagccg
    13561 cattcttcgg catgttcgca ggtatttatc attggttccc caaaatgtac tcccgtttta
    13621 tgaatgaaac gatggggcgc atccacttct ggggcacgat ggtaggcgcg tatgctattt
    13681 tctggcccat gcactacaca ggtatggccg gcgtaccgcg tcgctactat gcgttcgata
    13741 cctttgaaac attccgccac ttcactgagc tcaaccagtt cattaccatt gcggctatta
    13801 ttaccttctt gtttcaggta gttttta
//
LOCUS       DAORLF010000058        13799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2372_length_13799_cov_1.969805, whole genome shotgun sequence.
ACCESSION   DAORLF010000058 DAORLF010000000
VERSION     DAORLF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_13799_cov_1.969805"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK198_06995"
     CDS             complement(<1..218)
                     /locus_tag="PK198_06995"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38516.1"
                     /translation="MPKLSPLGLSDFKQVIERGTYYVDKTAMVRAVVEGAGVQLHCRP
                     RRFGKTLAVDQLNLQIPVGSIFALLGENG"
     gene            391..>1614
                     /locus_tag="PK198_07000"
     CDS             391..>1614
                     /locus_tag="PK198_07000"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRF38517.1"
                     /translation="MTKYIFVTGGVTSSLGQGIISASLAKLLQARGLSVTIQKLDPYI
                     NVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQTVIEKE
                     RRGDYLGKTVQVIPHITDEIKRRIQLLGSLNKFDIVITEIGGTVGDIESLPYLEALRQ
                     LRWELGSDNCMVIHLTLVPYLSAAKELKTKPTQHSVKELLTAGIQPDVLVCRTEHPIN
                     MDIRRKLALFCNVDVGSVIESIDADSIYDVPLLMLKERLDVVVLTKLRLPDRKEPDMR
                     VWKRFLDKLKAPKHKIRIGLVGKYNELPDAYKSIHESFVHAGTVNDCKVKVIPIHSEQ
                     LEADYESVAEKLQGLDGVLVAPGFGERGIEGKIMAIRYVREQGIPFFGICLGMQCAVV
                     EFARHVLQLNGAASTE"
     assembly_gap    1615..1644
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1645..2022
                     /locus_tag="PK198_07005"
     CDS             <1645..2022
                     /locus_tag="PK198_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRF38518.1"
                     /translation="VIDLMPDQQNVTNKGGTMRLGAYACSLTKKSKAAEAYGKNEISE
                     RHRHRFEFNNEYKAALENAGLWASGINPDTGLVEIVELREHPWFVGVQFHPELKSRVE
                     APHPLFSAFVKAALKYHQSKTAE"
     gene            2026..3522
                     /locus_tag="PK198_07010"
     CDS             2026..3522
                     /locus_tag="PK198_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38519.1"
                     /translation="MRLRDSGPLIGCFLLVVGAVWLAQQHTFFWDTVQLGAKHADFFY
                     TTDFSQLLLPQELDSGHPPLFGLYLAGCWRLFGATLPVSHWAMLPFLWGIVALLFALA
                     RHFGSEKTAWWLPLLVAADPVMAAQSVLVSPDVVLVFFFLLTLWSVLKQKPVIQAIAL
                     AGLALISMRGMMTAAMLFVWQFLFFSCEPRAASCELGSSKPPLTQWMRLLLKQALVFL
                     PGALLAGGWLLWHYRQTGWIGYHPDSPWAPSFERVGEGGVARNGVILIWRLLDFGRIF
                     MWAGLLFLLWQHRPLRAVFQNKIQQQAAGLLLLALLFLTPTLLLYKALSAHRYLLPVF
                     LALTVWLYALAAQSPKYGLKVVVVSALLLFGGNFWVYPQHIAQGWDATLAHWPYYELR
                     RQALDFIEQEDLPLDSIGTAFPEIGPLHYRDLGGFMDAARDGISRRQRQLQHGFKTKD
                     LDADPYILWSNVMNDFSDAERAALQQDWQPLRRWKKGAVEMVLYGRDL"
     gene            complement(3681..>3936)
                     /locus_tag="PK198_07015"
     CDS             complement(3681..>3936)
                     /locus_tag="PK198_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38520.1"
                     /translation="IRIMTVSGRIVHELTQDDLGPLRIGTHTTEQAWDGTDMYGDRLA
                     NGVYLYQVFAKDREGKDLIRHDTGTDAFFKGGIGKLVILR"
     assembly_gap    3937..3955
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3956..>4460)
                     /locus_tag="PK198_07020"
     CDS             complement(<3956..>4460)
                     /locus_tag="PK198_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38521.1"
                     /translation="QHLFNNNGLKDFFVGKDRRNPLLDVTFDGQRIMDGDIVSAKPLI
                     VIALRDENRWLRLDDTSLFKVLLRAPDAPDVRSVAIGSDEMRFLPAAGAGDNRATLEW
                     SPQFSESGLYTLIVQGRDATGNASGQVDYRINFEVITEARISNVLNYPNPFTTSTRFV
                     YTLTGEEA"
     assembly_gap    4461..4470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4471..>4819)
                     /locus_tag="PK198_07025"
     CDS             complement(<4471..>4819)
                     /locus_tag="PK198_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38522.1"
                     /translation="TFRTAPQIPYWRVLFDELPESVLNPALAFSFHKDSLQQGEPLSM
                     QVAVNNITAIGMDSLLLRFTVTDQAGNRQVVLRKTTPLPANDTIMAALQLDTRQMAGP
                     QQLRIEVNPDRAQP"
     assembly_gap    4820..4949
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4950..>6592)
                     /locus_tag="PK198_07030"
     CDS             complement(<4950..>6592)
                     /locus_tag="PK198_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38523.1"
                     /translation="YSQSYLNLLNSSHYGRGLGDVLRKVTEQYAAANGLPILALVHQF
                     TLHGDPAVVISPPDKPDLLLRTSDASVAPRSISVQTDSLELSLSVRNIGRVVGDSCVL
                     EIVRELPGGVQVSVLRQRISVPKLADRYVFRLPVWGDRGVVGLNRLLVKIDADNEIEE
                     GPGSGAEQNNNLADAQGREGIDFVVFANGAIPLYPPDMGIAGGDTLTLHASSADAFAP
                     ELQYFIEVDTTMYFDSPDKRSFTTTQTGGLISIRPDMAWRDSTVYYWRISPDSLPGIG
                     YTWRSSSFLYLAGSTGGWNQSHFFQWAGNRYINTELSESNRRLRFLDDAKFIRIKNGV
                     SPGFDNIGVDVNSYPHSFYDPAWDGSVSRRVYIFSLGSITAEPWTNPAPGRYGSHQPV
                     SWAPEHGNFPYWTTTPEWRQKAINFLRDTIPSGNYVILYTAQHNNQSYLPEAWAADSV
                     AFGTNLFQVLEQQGAQLIRSTAEIGSRPYFIMYKKDDPSFPVIERISLDNQPIFENII
                     LNGRWDRGEVRTNRIGPARNWRSLHWKIAENTAEDEWSL"
     assembly_gap    6593..6614
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6615..8687)
                     /locus_tag="PK198_07035"
     CDS             complement(<6615..8687)
                     /locus_tag="PK198_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRF38524.1"
                     /translation="MQKTTAIVLALLCSLCALSLRAQMWNGADTIYGHEWIRYDRPYF
                     KILLADDGMYRIPAATLTAQGVPLETLTGAQFQMFYMGQEEPLFVSTDGTFGANDFIE
                     FYGRRNRGELDRHLYRNPDAEMLNPLYSISTDTSAYFLTWVNPGTPTLRYQTLNNDLS
                     NPPPKEEWFWDERRQVFFNAVLQKADSEGVSESRFDEGEGYASNFSTTGNISFVPKFI
                     HPAPVNSTFRMRMFSNNRLHDLRISVNGDTLYRETFNGYRLRDYTVEKPTANLSANEQ
                     LRFNGIASGNDRHAVAWASLRYPRSYNFDNESSFAFQLPAASQDRYLEISNFNAGTAP
                     VLYDLTRRTRTVANVNARLIIPASNTPVNLLLVNASAGVRTVSNIRPLTFVNYQNADA
                     EYLIISHPRLMDDGNGGNPVQAYADYRASQAGGGFRTVVLDVTQLYDQFAYGVHRHPL
                     AVRNAVQYLARRWPEAKYAFIIGKGRQYNVGRTAAAVAGDYYVPTFGVPGSDNLLIAG
                     NWTNVPVLPIGRIAASTPAEVRWYLDKVISHEAGRNSTAEADREWKKEVIHLGGGGTG
                     EQQFIKNALGQMENIVRNGAMGANVQSFFKTNSDPIQQAVSDALLRRINDGASIITFF
                     GHSSSVGFDFAIDDPATYQNAGRFPAMISLGCHSGQIHEGLRSVGEDFIFQSNKGAIA
                     FISTTGYGY"
     gene            8886..9251
                     /locus_tag="PK198_07040"
     CDS             8886..9251
                     /locus_tag="PK198_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF38525.1"
                     /translation="MRTLAILLLESEPVVALDLKGELTRMGFAVHQAFRLSEAIEICQ
                     NLLPDIAFINFRQNQLTDGMSIARLLRVQYQMKVLLVTGARTRDLEAAGDFYAGQEVL
                     NKPFTRRQFRQAMTTLLKK"
     gene            9434..>10403
                     /locus_tag="PK198_07045"
     CDS             9434..>10403
                     /locus_tag="PK198_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38526.1"
                     /translation="MFRKPILVFATVLFSWGMQAQPSAVDSLSALLPSLPDGAQKIEV
                     LNQLLKLYLNSDLEKSLQYAHQVAGLSKKTGNRLALATVYKDLGVIHLIGSAYDSSQY
                     YSRLGLREYDVLLTKSSGEELEKVREGYAGTISNLGNWHYYQSALDSAVVYHRQAVEL
                     GRKWGADKPAANSLSTLAYIYLDQSQYEQAIEMHLEALRIFEKLDNQDGISRSWQGIG
                     EINCEYLNKCKTALDYFRKALKIKQKTGSERGMGYVFRLIGGAYDKLEMIDSAYFYYQ
                     KTIATAEKLNDKRLLIDGYSAIVSIAEAVGMPEQEQLEINLKYIALA"
     assembly_gap    10404..10423
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10424..>10778
                     /locus_tag="PK198_07050"
     CDS             <10424..>10778
                     /locus_tag="PK198_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38527.1"
                     /translation="GREDGLFVGYSNLGNMYRNKGAYDKAVEYYQKAALSAESQKNYG
                     FLEKLYLKQYEIFKTKLRDESKALAALEAYLVNHDSVSNAAKFQAVEDISTRYETEKK
                     EALIAEQQEAMHRDRI"
     assembly_gap    10779..10844
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10845..11494
                     /locus_tag="PK198_07055"
     CDS             <10845..11494
                     /locus_tag="PK198_07055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF38528.1"
                     /translation="GLTRQLRKRNREKEFLIKEIHHRVKNNLQVLSSLLHLQSRHIQD
                     EAALDAVREGQNRVDAMSLIHQKLYMGDNLAAVDMRDYLHNLGDTLLDAFGLEDDRVR
                     IVCHVEALRLDVDTAIPLGLIVNELVTNSLKYAFPGGRQGVVEISLWKNEADKLCLKV
                     ADDGVGQADAPALKNSTSFGTGLVEILSKKLKGKAQVLHENGYATLIEMEQFRLG"
     gene            11749..>11935
                     /locus_tag="PK198_07060"
     CDS             11749..>11935
                     /locus_tag="PK198_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38529.1"
                     /translation="MKTFDLVQHACSVSRTFTRFCIPKEKRRSISVVKFTASISIQLL
                     VLVIAIWPAQFIQAQCVA"
     assembly_gap    11936..11956
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11957..>13183
                     /locus_tag="PK198_07065"
     CDS             <11957..>13183
                     /locus_tag="PK198_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRF38530.1"
                     /translation="DDDCDSFVDEYAFSNTTAVPFTSTASSDIAVSGLAGTVTGVRIS
                     ELVINHTWIGDLSLVLRAPTGQTITLLDRPGIPASTFGCSADNIAVSFSSNASIPAEN
                     QCNLNPPGISGNFLPVGSFSTFNGLNPNGNWTLLITDNFVAGDNGTLIRWSLELITNV
                     AQSCCPAAGNIWYVKETAAAGGTGTSWQCAFKDLQLALAAASSGDQIWVAAGTYKPTS
                     GTDRNISFSMKNGVGIYGGFNGTETQLSQRNWRTNVTTLSGDIGISGNNSDNSYSVIV
                     NEGINSAIFDGFTVTQANTSPPTGNLNGGGIYNAGSSPTIRNCIFSNNTGSYGNAGIF
                     NFNNGTPTIINCLFINNVGTVYSSGVFNFSGNANIINCTFYGNHTAISNQGGNLISVS
                     NCILWGNNNSLNIISPP"
     assembly_gap    13184..13204
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13205..>13799
                     /locus_tag="PK198_07070"
     CDS             <13205..>13799
                     /locus_tag="PK198_07070"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRF38531.1"
                     /translation="ANSIVQGGWSGTGNLDTDPLFVNAAGGDFRLQACSPAINAGSNG
                     DVPGGITTDLDGNARFYNNGTVDMGAYEFQSTPPTFVTCYQDSDNDSFGNPAMSQVFC
                     NTCGTGYVSNSADCNDANQAINPLAAEVCDGFDNNCNSQTDEGNVCCPSGNVIYVKAN
                     ATGANNGTSWNNAYTNLQSALNSTCLGITEIWVAAGTY"
BASE COUNT         3080 a         3513 c         3807 g         3060 t
ORIGIN      
        1 ccattctctc ccagcagcgc gaagatggag cccaccggga tctgcagatt gagttgatcg
       61 accgccagcg tcttgccgaa gcggcgcgga cggcagtgga gttgtacgcc tgcgccctcc
      121 accaccgcgc gcaccatagc ggttttgtcc acatagtaag tgcctctttc tatgacttgt
      181 ttgaaatctg acaggccgag tggcgatagc ttgggcatgc tgaatgaatt ttacgacaaa
      241 gatacgaaat cttgcaggac acgcagaaag gggctatcca cataggagcc tgtctgattt
      301 tttgataaaa atgtaaatct gtttttttat cgccccctac cccaaaaaat aaattacctt
      361 tacggcccgt aacgtcacta ccacatcact atgaccaaat acattttcgt tacgggaggc
      421 gtcacctctt ctctcggcca aggcattatt tccgcgtcgc tggccaagct cctgcaagcc
      481 agaggtttgt cggttaccat ccaaaaatta gacccgtaca tcaacgtgga ccccggcacc
      541 ctcaatccct acgagcatgg cgagtgctat gtgaccgacg acggcgccga aaccgacctc
      601 gatctgggcc actacgagcg tttcctcaat gtgccgacct cgcaggccaa caacgtgacc
      661 acaggccgca tttaccaaac cgtcatcgaa aaagaacgcc gcggcgacta cctcggcaaa
      721 accgtgcagg tcatcccgca catcaccgac gagatcaaac gccgcatcca actgctcggc
      781 tcgctcaata aattcgacat cgtcatcacc gaaatcggtg gcactgtcgg tgatatcgaa
      841 tcgctgccgt acttagaagc gctccggcaa ttgcgctggg aactcggctc cgacaattgc
      901 atggtcattc acctcacttt ggtgccctat ctgagcgccg ctaaagagtt gaaaaccaag
      961 cctacacaac actctgtaaa agaactcctc accgccggca tacagcccga cgtattggtg
     1021 tgccgtaccg agcatcccat caatatggac attcggcgca agctggcgct gttttgcaac
     1081 gtggacgtcg gttccgtcat cgaatccatt gacgccgaca gcatctacga cgtgccgctg
     1141 ctcatgctca aagagcgctt ggatgtggtg gtgctgacca agctccgcct ccccgaccgc
     1201 aaagaacccg atatgcgcgt gtggaagcgt tttttggaca aactcaaagc gcccaaacac
     1261 aaaatccgca tcggcctggt aggcaaatac aacgaacttc ccgacgcgta taagtctatc
     1321 catgagtcat ttgtacacgc cggcacggtg aatgactgca aggtgaaagt cattcccatt
     1381 cattccgaac aattggaggc cgactacgaa tcggtggctg aaaaattgca gggtttggat
     1441 ggcgttttgg tagcgcccgg ctttggcgaa cgcggcatcg aaggcaagat catggccatt
     1501 cggtatgtgc gcgagcaggg cattcctttc tttgggatat gtctgggaat gcagtgcgcg
     1561 gtggtggaat ttgcccgcca cgttttgcaa ctcaacggcg ccgcttccac agaannnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnngtcatt gatctgatgc ccgaccagca aaatgtgacc
     1681 aacaaaggcg gcaccatgcg cctcggcgcg tatgcctgct ccttgaccaa aaagagcaaa
     1741 gctgccgagg cttacggcaa aaatgagatc agcgagcgcc accgccaccg cttcgagttc
     1801 aataacgagt acaaagcagc gctggaaaac gccggtcttt gggcatcagg catcaacccg
     1861 gacaccggct tggtggagat tgtggaactg cgcgagcacc cttggtttgt gggcgtgcag
     1921 tttcaccccg aactcaaaag ccgggtagag gcgccgcacc ccttgttctc cgcttttgta
     1981 aaggccgcgc tcaaatatca ccagagcaaa accgccgaat aagccttgcg cctgcgcgat
     2041 tccggcccgt tgattggatg cttcctcctc gtcgttgggg cggtgtggct ggctcagcaa
     2101 cacactttct tctgggatac ggtgcaattg ggcgccaagc acgccgactt tttctatacc
     2161 actgattttt ctcaactatt gctgccacag gaattggaca gcgggcatcc gcctttgttc
     2221 ggactgtatc tggcaggatg ctggcggctg ttcggggcta ccttgccggt gagccattgg
     2281 gccatgctgc cttttttgtg gggcatcgtt gccttgctgt ttgcgctggc ccggcatttc
     2341 ggcagcgaga aaacagcgtg gtggctgccg ttgctggtag ctgccgatcc ggtgatggcg
     2401 gcgcagtccg ttttggtcag tcccgatgtg gtgttggtgt tttttttcct cctgacattg
     2461 tggagtgtcc taaagcaaaa gcctgttata caagccattg ccctcgccgg gctggcgctc
     2521 atcagtatgc gcggcatgat gacggcggcc atgttgtttg tgtggcaatt tttgttcttt
     2581 agctgcgagc cgcgagccgc gagctgcgag ttgggttcat ctaagccgcc attgactcaa
     2641 tggatgagac tgctgttaaa acaagcgctc gttttcctgc ccggcgcgct gttggcgggc
     2701 ggctggttgc tgtggcatta ccgacaaacc ggctggatcg gttatcaccc cgactcgccc
     2761 tgggcgccga gcttcgagcg ggtaggtgag ggcggcgttg cgcgcaacgg cgtcattttg
     2821 atttggcggc tgctcgattt cgggcggatt tttatgtggg cgggcttgct gtttttgttg
     2881 tggcaacacc gaccgctcag ggctgttttt caaaataaaa tacagcaaca ggccgccggg
     2941 ttattgcttc ttgccttgtt gtttctcacg cctactttgc tgttgtacaa ggctttgagc
     3001 gcgcaccggt atttgttgcc ggtgtttttg gcgctcaccg tatggctgta cgcgctggct
     3061 gcacagtcgc ccaaatacgg cctcaaagtc gtggtggttt ctgcgctgct gcttttcggc
     3121 ggcaattttt gggtctatcc gcagcacatt gcgcaaggtt gggacgctac cctggcgcat
     3181 tggccgtact atgaactccg ccggcaagcc ctcgacttta tcgaacagga agacctgccg
     3241 cttgactcca tcggcacggc cttcccggaa atcgggccgc tgcattatcg ggacctgggc
     3301 ggttttatgg atgcggcacg agatggaatc tcgcgccggc aacggcaact acaacatggc
     3361 ttcaaaacaa aagacttgga tgctgacccc tacattttgt ggtcgaacgt gatgaacgac
     3421 ttcagcgatg ccgagcgggc ggctttgcag caggactggc agcccttgcg ccgctggaaa
     3481 aagggcgcgg tggagatggt gctgtatggg agggaccttt gattttttag caaaagccta
     3541 catcggagct gcagcgagtc cgaaagaaat tacgatcaaa aacaaggacc gatagtattc
     3601 attttcaaag tcttgcgcag tgaaatgcct ggctgcttct tcggcaataa cctgcgcaga
     3661 gatgtcgccg gcgcgttcct ctatctcaaa atcaccaatt tcccaattcc tcctttaaaa
     3721 aacgcatccg tgccggtatc gtggcgaatg aggtctttac cctcgcggtc tttggcaaac
     3781 acctgataca aatacacgcc gttggccaag cggtcgccgt acatatcggt gccgtcccag
     3841 gcctgttcgg tggtatgcgt accgatgcgc agcggcccca gatcatcttg cgtcagttcg
     3901 tgtacaatgc gccccgaaac cgtcatgatg cgaatgnnnn nnnnnnnnnn nnnnngcttc
     3961 ctcgccggtg agggtataaa caaagcgggt ggaggtagta aatggattgg gataattgag
     4021 tacgttggaa atacgcgcct cggtgatgac ctcaaaattg atccggtagt ccacctgccc
     4081 ggaggcgttg ccggtcgcgt cgcggccctg cacgatgagg gtgtaaaggc cgctttcgct
     4141 gaattgcggc gaccattcca gtgtagcgcg gttgtcgccg gcgccggcag caggcaggaa
     4201 gcgcatctcg tccgaaccaa tggcaacgga gcgcacatcg ggggcatcgg gggcgcggag
     4261 cagcactttg aacaggctgg tgtcgtccaa tcggagccag cggttttcat cgcgcaaagc
     4321 aatgacaatg agcggcttgg ccgacacaat gtcgccgtcc atgatgcgtt gcccgtcgaa
     4381 ggtgacatcg agcagggggt tgcggcggtc ttttcctaca aaaaagtctt tgaggccgtt
     4441 gttgttgaac agatgctgtt nnnnnnnnnn gggctgtgcg cggtcggggt tcacttcgat
     4501 gcggagttgc tgcgggccgg ccatttggcg ggtgtccaat tggagggcgg ccatgatggt
     4561 gtcgttggcc ggcagggggg ttgtttttct gagaacgacc tgccggttgc ccgcctgatc
     4621 ggtgacggtg aaacgcagca acaggctgtc catgccgatg gcggtgatgt tgttgacggc
     4681 cacctgcatc gaaagcggtt cgccctgttg gaggctgtct ttgtggaaac tgaaagccag
     4741 cgcggggttg agtacagatt cagggagttc gtcaaagagg acgcgccagt acggtatctg
     4801 cggcgcggtt cggaaggtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aatgaccact cgtcttcggc tgtattttcg
     4981 gctattttcc aatgcaggga gcgccagttg cgtgccggcc cgatgcggtt ggtgcgcacc
     5041 tcgccgcggt cccagcgccc gttgagaatg atattttcaa agatgggctg attgtcaaga
     5101 gaaatccgct caatgaccgg gaagctcgga tcgtcttttt tgtacataat gaaataaggc
     5161 cgcgaaccga tctcggcggt gctgcgaatg agctgtgcgc cctgttgctc caatacctga
     5221 aaaagattgg taccgaaggc cacggagtcg gcggcccagg cttcgggcag gtagctttgg
     5281 ttgttgtgtt gtgcagtgta gaggatgacg tagttgcccg aggggatggt atcgcggagg
     5341 aagttgatgg ccttttggcg ccattcgggg gtggttgtcc aataggggaa attgccgtgc
     5401 tcgggcgccc aactcaccgg ctgatggctg ccgtagcgcc cgggagcggg gttggtccag
     5461 ggttctgcag taatggagcc aagtgaaaaa atatatacgc ggcgcgacac agagccgtcc
     5521 catgccggat cgtaaaaact gtgaggatag ctgttgacat ccacgccgat gttgtcaaaa
     5581 ccaggagaga cgccattttt gatgcgaatg aatttggcat cgtccaaaaa gcgaagtctg
     5641 cggttgcttt ccgacagttc cgtgttgatg tacctgttgc cggcccattg aaaaaagtgc
     5701 gattgattcc agcctccggt actgccggcg aggtacaaaa acgagctgct gcgccaagtg
     5761 tacccaatgc ctggcaagct gtcggggctg atgcgccagt aatacacagt gctgtcgcgc
     5821 caggccatgt cgggccggat gctgatgagg ccgccggttt gcgtcgtggt aaaactgcgt
     5881 ttgtccggac tgtcgaaata catcgtggta tctacctcga tgaagtattg caattcgggg
     5941 gcaaaagcat ctgcgctgga ggcgtgcaaa gtaagggtat cgcctccggc aatgcccata
     6001 tcgggtgggt aaagagggat agcgccattg gcaaacacca caaaatcaat gccttcccgg
     6061 ccctgagcat ctgccaggtt attgttttgt tcggcgcccg agccagggcc ttcctctatt
     6121 tcattgtcgg cgtcaatttt taccaacaaa cggttcaatc ctactactcc gcgatcaccc
     6181 catacgggca gacggaacac ataccggtcg gccaatttgg gtacggagat gcgctgcctg
     6241 agtaccgaca cctgcactcc gcccgggagc tcgcgcacaa tttccagcac acaactgtcg
     6301 cccaccaccc ggcctatatt tcgcactgaa agactgagtt ctaagctgtc ggtttgtacg
     6361 ctgatggaac gcggcgccac ggaggcatcg cttgtgcgca acaagagatc gggcttgtcg
     6421 ggcggactga tgacgacggc agggtctccg tgcagggtaa actggtgtac cagcgctaag
     6481 attggcagac cgttggcggc ggcgtattgc tccgtcactt tgcgcagcac atcgcctagt
     6541 ccgcgcccgt aatgactgct gttgaggagg ttgagatagc tttgagaata agnnnnnnnn
     6601 nnnnnnnnnn nnnnatagcc gtagcccgta gtggagataa aagcaatggc gcctttgttg
     6661 ctttgaaaaa taaaatcctc gcccacactg cgcagccctt catgaatctg gccagaatgg
     6721 cagcccagtg agatcatggc cggaaagcgc ccggcatttt ggtaggtggc aggatcgtct
     6781 atagcaaagt caaaacctac gctgctggaa tgtccgaaaa aggtaatgat ggaggcgccg
     6841 tcgttgatgc gtcgcaacag cgcatccgac acggcctgct gaatggggtc agagttggtt
     6901 ttgaagaacg actgcacgtt ggcgcccatg gccccgttgc gcacgatgtt ttccatctgg
     6961 cccagcgcat ttttgatgaa ctgctgctcg ccagtgccgc cgccgcccag atgaatgacc
     7021 tcttttttcc actcgcggtc ggcctcggcg gtagagtttc ggccggcttc gtgggagatg
     7081 actttatcca aataccaacg cacttcggcg ggtgtagaag cggctatcct gccaatgggc
     7141 aataccggca cgttggtcca gttgcccgca atgagcagat tgtccgaccc cggcacgccg
     7201 aacgtaggca cataataatc gccggccaca gcagcagccg tgcgccccac attgtactgc
     7261 cgccctttgc ctatgatgaa ggcgtatttg gcttccggcc acctgcgcgc caggtattgc
     7321 acggcattgc gcacagccaa cgggtgccgg tggacgccgt aggcaaactg atcgtacagt
     7381 tgcgtcacat cgaggacaac cgtgcggaaa ccgccgcctg cctgcgatgc gcggtagtcg
     7441 gcgtaagcct gcacgggatt gccgccgttg ccgtcgtcca tcaggcgggg atgcgaaatg
     7501 atgaggtact cggcatcggc attttggtaa ttgacaaaag tgaggggccg gatattgctc
     7561 accgtgcgca caccggcaga ggcattcacc aacagcaggt tgaccggggt attggaggcg
     7621 ggtatgatga gccgcgcatt cacgttggct acggtgcggg tgcggcgcgt gaggtcgtac
     7681 aaaacgggcg ctgtgccggc attgaagttg ctgatttcca agtatctgtc ttgtgaggcg
     7741 gcgggcagtt ggaaggcgaa ggagctttcg ttatcaaagt tgtacgacct cggataacgc
     7801 aggcttgccc aggctacggc gtggcggtcg ttgccggaag cgatgccgtt gaagcggagt
     7861 tgttcgttgg cactgaggtt ggcggtgggt ttttctacgg tgtaatcccg cagcctgtat
     7921 ccgttgaaag tttctctgta aagtgtgtcg ccgttgacgg aaatgcgtag atcgtgaaga
     7981 cggttgttgg aaaacatgcg catccggaaa gtgctattta ccggcgccgg atggatgaat
     8041 ttggggacaa aactgatatt cccagtggtg gaaaagttcg aagcatagcc ctctccttca
     8101 tcaaagcggg actcagaaac gccctcgctg tcagcttttt gcaatactgc attgaagaac
     8161 acctgcctcc gctcatccca aaaccattct tctttgggcg gaggattgct caggtcgttg
     8221 ttcagggttt ggtagcggag ggtgggcgtg ccaggattca cccaggtgag gaagtatgcc
     8281 gaggtgtcgg tactgatgct gtacagtggg ttcagcattt cggcgtcggg attgcggtag
     8341 aggtggcggt ccaattcgcc gcggttgcgc ctgccgtaaa actcgatgaa gtcattggcg
     8401 ccgaaagtgc catcggtgct cacgaagagc ggttcctcct gccccatgta aaacatctga
     8461 aactgagcgc cggtaagggt ttccaaaggt actccctgcg cagtgagggt agccgccggg
     8521 atgcggtaca tgccgtcgtc ggccaatagt attttgaaat aaggccggtc gtagcgtatc
     8581 cactcgtggc cgtagatggt gtcggcgccg ttccacattt gggcgcgcag gctcagagcg
     8641 caaagactgc acaacagagc gaggacaatg gcggtagttt tctgcatcaa aggcataagg
     8701 ctgttgggtt gaatcggacg gaaaaacaga gcgataacac acagagtagg gtaggggttt
     8761 tgtgtgccga agaagtatgg gcgcaatgga gcgaaactgt ccaaaacggc actttcactg
     8821 gccaataccg tccattcact ggaaaaaatg tattagctac tctggcatct cagtaccttg
     8881 tcgggatgag aacacttgcc atcctgctgc tggagtctga accggttgtt gcccttgatt
     8941 tgaaagggga actgaccagg atgggctttg ccgtgcatca ggcttttcgg ttatcggagg
     9001 ccatagagat ttgccaaaac ctgctgccgg atatagcgtt catcaatttt cgacagaacc
     9061 aactcaccga cggcatgagc atagcccgcc tcctgcgcgt gcagtatcaa atgaaagtat
     9121 tgcttgtcac cggcgcccgg acccgtgatt tggaggctgc cggagatttt tatgccggcc
     9181 aggaagtttt gaacaaaccc tttacccggc ggcaattccg gcaggctatg acaacactgt
     9241 taaaaaagta gcggactctt atctgcctac gctaaagctt cggcggatga aaaagaataa
     9301 gtattggagg atttttagga agcagtcggg gcggtggttc cggctgcctt ttgtattagg
     9361 gataggaaaa atctttatct tcgtggcggg ctattgcgcc gaatagaaga gttccaaaaa
     9421 aaccatattt gtcatgttta ggaaacccat attggtgttt gcaaccgtgc ttttttcatg
     9481 gggaatgcaa gcgcagcctt cagccgtgga cagcctgagt gccttgctgc caagcctgcc
     9541 cgatggcgcg cagaagatcg aagtactgaa tcaattgctg aaactatacc tgaattccga
     9601 cctggagaag tccctgcaat atgcccatca ggtggcgggg ctgagcaaaa aaacgggcaa
     9661 tcgcttggcg ttggctacgg tttacaagga tttgggggtg atacatctca ttggttcggc
     9721 ctacgattca tcgcaatatt acagccggct gggcttgcgg gaatacgatg tcttgttgac
     9781 aaaaagcagc ggtgaagaac tcgaaaaagt gcgcgaaggc tacgccggaa cgatcagcaa
     9841 cctcggcaac tggcactact accaatcggc gctcgactcc gccgtagtgt atcatcggca
     9901 ggcggtggaa ctgggccgaa aatggggcgc tgataaaccc gccgccaact ccctgagcac
     9961 gttggcctac atctacctcg atcagtcgca atacgagcag gccatcgaaa tgcacctcga
    10021 agcgctgcgc atttttgaaa aattagacaa tcaggacggc atttccagaa gctggcaggg
    10081 catcggggag atcaactgcg agtatctgaa caaatgtaag actgctttag attactttcg
    10141 caaggcgctg aaaatcaaac agaaaaccgg cagcgaacgg ggcatgggct atgtttttcg
    10201 gctgatcggg ggggcttatg acaagttgga aatgattgac tcagcctatt tttattacca
    10261 aaaaacgata gcaaccgccg aaaaactgaa cgacaaaagg ctgctcatag acggctattc
    10321 cgccatcgta tccatagcgg aggcagtggg tatgccggag caagagcagc tcgaaatcaa
    10381 cctgaaatac attgctctgg cggnnnnnnn nnnnnnnnnn nnncgggcgg gaagacgggc
    10441 tgtttgtggg gtattccaac ctcggcaata tgtaccgcaa taaaggcgcc tacgacaagg
    10501 ccgtggagta ctatcagaaa gcggccctgt cggcggaaag ccagaaaaat tatggctttt
    10561 tggaaaagct atacttgaag cagtatgaaa ttttcaagac aaagctccgg gacgaatcca
    10621 aagcactggc ggccctcgaa gcgtatttgg tcaaccatga cagcgtatcg aatgccgcca
    10681 aatttcaggc agtggaagac atcagcaccc gctacgaaac ggagaaaaaa gaagctctca
    10741 ttgccgagca acaggaggcc atgcaccgcg accgcattnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggggcc tgacccgcca
    10861 attgcggaaa cgcaaccggg aaaaggaatt tctgatcaaa gaaatccacc accgggtgaa
    10921 gaacaacctg caagtgctgt cgagcctgct gcacctgcaa tcgcggcaca tacaggacga
    10981 agccgcctta gatgccgtgc gcgagggtca aaaccgcgtg gacgccatga gcctcattca
    11041 ccaaaagctg tacatgggcg acaacctcgc cgcagtggac atgcgcgact acctgcacaa
    11101 cctcggagat accctgctgg acgctttcgg gctggaggat gaccgcgttc gtatcgtctg
    11161 ccatgtggaa gccctgcgtt tggatgtgga caccgcgatt cccttggggt tgattgtcaa
    11221 tgaattggtg accaattcgc tgaaatacgc cttcccgggc gggcggcagg gcgtggtaga
    11281 aatttcgctg tggaaaaacg aagcagataa actttgcctg aaagtggccg acgacggcgt
    11341 ggggcaagcc gatgcgcccg cactgaaaaa cagcacttct ttcggcaccg gcttagttga
    11401 aattctgagc aaaaaactaa agggcaaggc gcaggtattg cacgaaaacg gctacgccac
    11461 cctcatcgaa atggagcagt ttcggctggg ctgaaaccag cttccctgtg agattccggt
    11521 cattcactgg ctattcggct actttcactg aataaatttt cattcctcct ctctctggtt
    11581 gcaatttcac ctttgaaatc tgtgccgatg ccgttttttc tgttgcctga aagtggaaaa
    11641 aacggcacaa cgttgcatcg cagccccgct cctctgcgca ggggggcgga ggcgggcggt
    11701 gtgggttgga ggctcatttt acaatttacc ttttaaaacc gttgtgccat gaagacattc
    11761 gatttagttc aacacgcttg ttctgtatcc agaacattca ctcgcttctg tattccaaag
    11821 gaaaaacgca gaagcatctc cgtggtaaag tttacagcct caatatccat acagcttctc
    11881 gtcttggtga tcgctatctg gcccgctcaa ttcatacagg cgcaatgcgt tgctgnnnnn
    11941 nnnnnnnnnn nnnnnngacg atgactgtga cagttttgta gatgagtacg cattcagcaa
    12001 cacgacggca gtacccttta ccagtaccgc ctcctcagac atagcggtaa gcggactggc
    12061 cggcacggtt accggcgttc ggatatccga gttagtgatt aatcatactt ggataggcga
    12121 cttgagctta gtcttaaggg cgcccaccgg acaaacgata accctacttg accgccccgg
    12181 aattccagcg tccacgtttg gttgcagcgc cgataatata gctgtgtcat tcagcagcaa
    12241 tgccagcatt ccagccgaaa atcaatgtaa cttgaaccct cctggcatat ccggcaactt
    12301 cctgcccgtc ggtagttttt ccacctttaa cggcctcaat cccaatggca actggacgct
    12361 gcttattacc gataactttg ttgcaggcga caatggtacg ctcatccgtt ggtctttgga
    12421 gctgataacc aatgtggccc agagctgctg cccggcggcg ggtaatatat ggtacgtcaa
    12481 agaaacagcc gccgccggcg gcacaggtac ttcctggcag tgcgccttca aggatttgca
    12541 gctcgcgctg gcagcggcaa gtagcggcga tcagatatgg gtagctgccg gcacttacaa
    12601 gcccacctcc ggcacagacc gcaatatttc cttctccatg aaaaacggcg tgggcatcta
    12661 cggcggcttc aacggcacgg agacgcagct ttcacaacga aactggcgga cgaacgtgac
    12721 cacgctgagc ggggatattg gaatttcggg gaataattca gataacagct atagtgtaat
    12781 cgttaatgaa ggaatcaatt ctgccatatt tgatggattt acggtaacgc aggcaaatac
    12841 ttctcctcca acgggaaacc tcaacggcgg aggaatttac aatgctgggt catctcccac
    12901 tattagaaat tgtatttttt ccaataacac gggcagttat ggaaatgcag ggatatttaa
    12961 tttcaataat gggactccaa cgattatcaa ttgccttttt atcaataatg tagggactgt
    13021 gtattcctct ggagtgttta actttagtgg caatgcaaac ataataaatt gtacatttta
    13081 tggcaatcat acggctattt caaatcaggg ggggaatcta atatccgtta gcaattgcat
    13141 cctctgggga aataataact cacttaatat tatatccccc cctnnnnnnn nnnnnnnnnn
    13201 nnnntgctaa ttctattgtc cagggcggct ggagtggcac gggcaacctc gataccgatc
    13261 cgctgttcgt caacgccgct ggcggcgatt tccgcctgca agcctgctcg cctgccatca
    13321 acgcgggtag caatggcgat gtgccaggtg gtattaccac cgatttggac ggcaatgctc
    13381 gcttctacaa taacggaacg gtggacatgg gggcgtatga attccagagc acacccccta
    13441 ccttcgtcac ctgctaccag gacagcgata atgacagctt cggcaatccg gccatgagcc
    13501 aggtgttttg caatacctgc ggcacggggt atgtttcaaa tagcgccgac tgcaacgacg
    13561 ccaaccaagc catcaatccc ctcgctgccg aagtctgcga tggcttcgac aacaattgca
    13621 acagccaaac cgacgagggc aacgtttgct gcccatcggg taatgtgatt tatgtaaaag
    13681 ccaacgccac cggcgccaac aacggtacgt cctggaacaa tgcctacact aatctgcaaa
    13741 gcgcactgaa cagtacctgc ctgggcatta ccgaaatctg ggtggccgcc ggtacttac
//
LOCUS       DAORLF010000059        13572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2420_length_13572_cov_1.752608, whole genome shotgun sequence.
ACCESSION   DAORLF010000059 DAORLF010000000
VERSION     DAORLF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13572
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_13572_cov_1.752608"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>936
                     /locus_tag="PK198_07075"
     CDS             <1..>936
                     /locus_tag="PK198_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF38532.1"
                     /translation="SKDAGQTWEKINTGLPTVDIGRIGLAMAPSDPEVIYAIVEAAQG
                     KGGFYRSTNRGASWEKRGGHSTSGNYYSEIVVHPHNPDVVYSMDVWIQISNDGGKSFQ
                     MMGEEFKHVDNHSMWIDPKDPNYYLVGCDGGIYESFDAAKTWVFKANLPVTQFYKVAV
                     DNSSPFYYIYGGTQDNFSLGGPSRTRSAHGIVNEDWFVTNGGDGFESAIDPKNPNIVY
                     AQAQHGVLVRYDRESGEATGIQPKPGKGEPELRWNWDSPLMTSAHVDKRLYFAANKVF
                     RSDDQGNSWKAISPDLTRQLDRNTLPVMGRIQSMDA"
     assembly_gap    937..983
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <984..>1336
                     /locus_tag="PK198_07080"
     CDS             <984..>1336
                     /locus_tag="PK198_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF38533.1"
                     /translation="FHESPMDANLLYVGSDDGLIHISEDGGANWTKVDCNAIPGVPER
                     TYVNYLLASQHDRNVVYAAFNHHKYGDFKPYFYKSSDRGRTWKAMSNNLPERGSVYSI
                     AEDHVDKDLLFVGTE"
     assembly_gap    1337..1536
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1537..>1862
                     /locus_tag="PK198_07085"
     CDS             <1537..>1862
                     /locus_tag="PK198_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38534.1"
                     /translation="PIKDGLVYHESYPLGLRGKAFQGHAYFTAPNPPVGAAFTYFVKD
                     EVKTLKQKRQEDEKEKIKKGEPIRYPTYEELEAETREEEPYLLFTIRDLSGSIVRELK
                     TGISKG"
     assembly_gap    1863..2074
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2075..>2462
                     /locus_tag="PK198_07090"
     CDS             <2075..>2462
                     /locus_tag="PK198_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38535.1"
                     /translation="PAADRAELVAFQRQAQDLQRVVSGAGSMLGEINNRMQHIRKAIF
                     SVGAPKSELVNDFKAIEAKLYAIRKTMYGDNIAATLDKDDPFTVASRIGWLTGEMWGS
                     TSAPTQTQRDALRIADEEFKPLLEQIR"
     assembly_gap    2463..2477
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2767..3138)
                     /locus_tag="PK198_07095"
     CDS             complement(2767..3138)
                     /locus_tag="PK198_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRF38536.1"
                     /translation="MKAWYTIALLVLSNAFMTLAWYGHLRFKGIKWFEELHIFWIILI
                     SWGIAFFEYMLQVPANRIGFKEYGGPFSLIELKVIQEVLTLLIFMAFTLIFFKTETFK
                     WNHLVGFVFLVLAVFFVFKKW"
     gene            3182..3667
                     /locus_tag="PK198_07100"
     CDS             3182..3667
                     /locus_tag="PK198_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38537.1"
                     /translation="MEQIIEVFEPLKSPWREMALPLLSAVLLVIAQVLQLRNKNIERN
                     RRIVFSMLLFFGAVIALLSAGGIAINAYKFQRVTVSTSAVSIGKKEIPYSNLRGYLIR
                     EDREQSWVNSDIQKKAVRSLVLEEKNGKTYLLPEEYYELSHLIATLDAAFSQWKEVGR
                     Q"
     gene            3828..>4113
                     /locus_tag="PK198_07105"
     CDS             3828..>4113
                     /locus_tag="PK198_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38538.1"
                     /translation="MILLQIAAGLIFILVLFTLLATAIQELIANYISLRGVMLMKTIQ
                     HILEPNLKGIRGEVYNSFIKSLSFKQLCRPSSSSAYGRFLDWLEEKTRYIF"
     assembly_gap    4114..4175
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4198..>4428
                     /locus_tag="PK198_07110"
     CDS             4198..>4428
                     /locus_tag="PK198_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38539.1"
                     /translation="MLKRLEWKPEYLESIPPGPLRDYLDNLGEQAEDKVQARLSDLKR
                     WYDEVMNATTNWYRRQAQFILIFIGLALAASFN"
     assembly_gap    4429..4459
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4460..>4793
                     /locus_tag="PK198_07115"
     CDS             <4460..>4793
                     /locus_tag="PK198_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38540.1"
                     /translation="GKTLSKDEILRTQLYSDMEKWMAERADTYFDENGQVRQGVLVTQ
                     NDKNIIVVDPNLKDDLKQSDAVQQLIGKKASFATVGDWLYNIANWLLTAIFISFGAPF
                     WFDLLRKLV"
     assembly_gap    4794..4803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4951..>5174
                     /locus_tag="PK198_07120"
     CDS             4951..>5174
                     /locus_tag="PK198_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF38541.1"
                     /translation="MNNEKILIIGANGQIGTVLTQALRDLYGQDQVIASDLAAVPTMP
                     AGPFETLDVLNAVRLGEIVRQYGIGQVYHLA"
     assembly_gap    5175..5189
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5190..>5409
                     /locus_tag="PK198_07125"
     CDS             <5190..>5409
                     /locus_tag="PK198_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF38542.1"
                     /translation="SAKGEQHPKWAWEINMGGVFNVLEAAREYRLKVFFPSSIASFGP
                     FAERQNTPQHGPLEPTTVYGISKVAGELW"
     assembly_gap    5410..5624
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5625..5900
                     /locus_tag="PK198_07130"
     CDS             <5625..5900
                     /locus_tag="PK198_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRF38543.1"
                     /translation="MEAPAEQVKQRTSYNLAGMSFTPAEIAAEIQKHYPEFRVSYQPD
                     FRQAIADSWPESIDDADARNDWGWAPQFDLASMTAEMLSRLKEKYAR"
     gene            5903..>8290
                     /locus_tag="PK198_07135"
     CDS             5903..>8290
                     /locus_tag="PK198_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38544.1"
                     /translation="MSYAFSLPFHIFRLKFPNKHGDHVLRPVADIGSVSLGESKHLLT
                     ERFAASFQQEVLNKGRAGELLDMSLYQDLKHTAIATPFAGSSDGVSYPAFEMESDAFI
                     GARNGTWWGIVPALGVEAAADSEELLLQSLADNVRVYFVRTGRLRAVQGIVSALWYEE
                     VSLESSDISLLSLAPSEVENQETNATRQLLSDAATPLPATRKELFGMEYELEQVLRAF
                     QNNFGRCVLLTGPGGCGKSAIVAEAARQWKTKHRAGQFWETTASRLIKELTRDTGWQY
                     NLSHLCRELNANGDWLYVRSLMELFEVGRYVGNEVSMGEFLRNYLSRGEIALIAECTP
                     EELQRIELISPNFSSFFSKVEVIPPKEKVLEGIIQNSVQQLSAERKIEFDKTAVREAI
                     ALHARFNPYSGMPGKVIRFLEGIIREPLRTGSQTSQRRITGKDIVERFCEESGIPALL
                     LNDDLPLEPDQILERFNSNVFGQPHAAAAVTDMLVKLKTRISKERKPIASILFAGPTG
                     VGKTELAKVLAELVFGDRNRMIRFDMSEYSGPDAVFRLTGAGDADGLLTSAVRKEPFC
                     VLFDEIQKASPDFYDMLLQILDEGRLSDNSGKLVNFCSAIIIMTSNIGAAGMKSKPIG
                     LKPGRSASNVREHYQDAVKKYFRPELVNRFDDIIPFNALDEAAIRFVVERELKLLLQR
                     EGIRYRRMNLHIDEGVADYLGRRGFDPQYGARYLQRAMREMLLLPLSKSLAEQDPTDH
                     LEVSVYMDAQGYRPAVKVESDPLSLELLIEELHHANITNHTSTLRRTIYAVEDGFL"
     assembly_gap    8291..8326
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8327..9168
                     /locus_tag="PK198_07140"
     CDS             <8327..9168
                     /locus_tag="PK198_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38545.1"
                     /translation="DRRSHPTGFWKDHERVREYNALSKFTERMNGLKTEIEMLEMNAS
                     VSFLGQASDDVRLPTQLTNWTSEAQVFKKDLVAHVNREYNSCVLGLYGVDLEKIYNMY
                     RVIVRRLGIQMSGVWPVWHRNSYYEELVSDPENPEVKVKREEYYYTEEEWFTGTSGDY
                     RQPTAEKSGDKLVGLEFKIDGPVAYLLMSGESGFQHWLLPGHTEPTPYWVQVMPFEQH
                     ATPKRVHRLDFFTAKPRRIMAPGTYSDKNYNIEAATESEFLDAVAQHCQAYMIQEVNL
                     AVM"
     gene            9205..>9710
                     /gene="nth"
                     /locus_tag="PK198_07145"
     CDS             9205..>9710
                     /gene="nth"
                     /locus_tag="PK198_07145"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRF38546.1"
                     /translation="MTRKAKAAAITAILERLYPETPIPLQHEDAYTLLVAVLLSAQCT
                     DERVNKVTPHLFALADNPQEMSQLPVQTIHDIIRPCGLAPRKSQAISDLSKILIEQHN
                     GRVPDNFEALEALPGVGHKTASVVMSQAFGVPAFPVDTHIHRLAWRWGLSSGKNVEQT
                     EADLKKLFP"
     assembly_gap    9711..9720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9862..>10267
                     /locus_tag="PK198_07150"
     CDS             9862..>10267
                     /locus_tag="PK198_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRF38547.1"
                     /translation="MFTGIIETAGRVVAMQQEGSNVHFTIASDISSQLKVDQSLAHNG
                     VCLTVTALGEGTHTVTAIEETLQRSNLGRLRPGDALNLERAMRSDGRLDGHIVQGHVD
                     TTGICTGVEELEGSWYFHFEYAPNPDHLLVDKG"
     assembly_gap    10268..10277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10278..10457
                     /locus_tag="PK198_07155"
     CDS             <10278..10457
                     /locus_tag="PK198_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRF38548.1"
                     /translation="VNGVSLTVVNPTEKTFSVAIIPYTYEHTNFKAIRSGDTVNLEFD
                     VIGKYIARYVRLYMG"
     gene            complement(10522..>10915)
                     /locus_tag="PK198_07160"
     CDS             complement(10522..>10915)
                     /locus_tag="PK198_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38549.1"
                     /translation="VTEPSMDRFQTIELNPRIGFYLTPDFSLQTGAYVSHILRAYNNM
                     GDGVWNKVPDWAAEFLYNDVDFGLTGGLKYAVRRFSLHGNVQFGLMTDHKFVPDPQFT
                     DVNGEPLETTGRNLAFQLGLGYTLIGKK"
     assembly_gap    10916..11017
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11018..11207)
                     /locus_tag="PK198_07165"
     CDS             complement(<11018..11207)
                     /locus_tag="PK198_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38550.1"
                     /translation="MKKPLLILAFATMFSGSLSAQFSLALQGGIQQSRYILSSNAPFL
                     FDPSQEPTPRIFQGGYHIG"
     gene            complement(11492..>12391)
                     /locus_tag="PK198_07170"
     CDS             complement(11492..>12391)
                     /locus_tag="PK198_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRF38551.1"
                     /translation="SFHVEQSSRFQNIFTILKLALMVFLALACFVHSAPINGLAWSGP
                     WLFELGTVPYAVALVFVVYAYSGWNAAAYITGEVRQPARNLPRALVGGALLVSVLYVL
                     LQMGFLYQAGREALAGKIEVAQIAAVSVFGASGGQTVSLLIGLLLVSSISAMVWVGPR
                     VSRAMAEDYPVWYFLARDNRHGVPVNAIWFQAAVSIFLLLSGSFEQVLTYSGFVLHLS
                     TTAAVAGLFFLRRKDKGRRPPFASPFYPWAQIIFLLFSVWMLAFLLYDKPRESLLGLL
                     NLAIGSVSYWWNKRYALPPVKGK"
     assembly_gap    12392..12434
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12435..12804)
                     /locus_tag="PK198_07175"
     CDS             complement(<12435..12804)
                     /locus_tag="PK198_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF38552.1"
                     /translation="MHPTTHRKIGWVSAGSIVVANMVGTGAFTTLGLQLQHIHSTWAI
                     LSLWVFGGVIALLGAVSYAELGARMPRSGGEYYYLSQIYHPLAGYLSGWISLTVGFTA
                     SIALSAMAMGAYLSEVVGTDA"
     assembly_gap    12817..12828
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12910..>13132)
                     /locus_tag="PK198_07180"
     CDS             complement(12910..>13132)
                     /locus_tag="PK198_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF38553.1"
                     /translation="AFLILIGFMLIAEGAHLAHLSIAGSEVGAVPKGYLYFAISFSLF
                     VEFLNMRLRKQHTPVQLHGAQNAEEPEKR"
     assembly_gap    13133..13241
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13242..>13572)
                     /locus_tag="PK198_07185"
     CDS             complement(<13242..>13572)
                     /locus_tag="PK198_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF38554.1"
                     /translation="IGLLLAMVMRIALLFGVSWLIAMEEPWIHFDQNWIKAGFSGQSL
                     ILIAGGLFLLYKSTTEIRHKLEGPDTVQGQSEDGKLKKTLSNVIVQITVINIVFSFDS
                     ILTAVGLT"
BASE COUNT         3085 a         3487 c         3316 g         2555 t
ORIGIN      
        1 tcgaaagatg ccggccaaac ctgggaaaag atcaacaccg gactgcctac ggtggacatc
       61 ggtcgcatcg gcctcgccat ggccccttcc gacccggaag tcatctacgc catcgtggag
      121 gcggcacagg gcaaaggcgg tttttatcgc agcaccaatc gcggcgcaag ctgggaaaaa
      181 cgcggcgggc acagcacttc gggcaattac tactccgaaa tcgtggtgca tccgcacaac
      241 cccgacgtgg tttactctat ggacgtttgg attcaaatca gcaacgatgg cggcaagagt
      301 ttccaaatga tgggcgaaga gttcaagcac gttgacaatc actcaatgtg gattgatccc
      361 aaagacccca actactacct cgtgggctgc gacggcggca tttacgagtc ttttgacgcg
      421 gccaaaacct gggtattcaa agccaacctg cccgtcacgc aattttataa agtagccgtt
      481 gacaactcct cgccattcta ctacatctat ggcggcacgc aagacaactt cagtctcggc
      541 gggccttccc gcacccgcag cgcacacggc atcgtcaacg aagactggtt tgtaaccaac
      601 ggcggcgacg gcttcgaatc ggccattgac cccaaaaatc ccaacatcgt gtatgcgcag
      661 gcacagcacg gcgtactagt gcgctatgac cgcgaaagcg gagaggccac cggcatacaa
      721 cccaagcccg gcaaaggcga acccgaactc cgctggaact gggattcacc tctgatgacc
      781 tccgcgcatg tggacaagcg gctctatttt gcggccaaca aggtgttccg cagcgacgat
      841 cagggcaatt cgtggaaagc catcagcccc gacctcaccc gacaattgga ccgcaacacc
      901 ctgcccgtca tgggccgtat ccaaagcatg gacgccnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnttccacg aaagcccgat ggatgccaat ctgctatacg
     1021 tcggctccga cgatggcctc atccacatct ccgaagacgg cggcgccaac tggacaaaag
     1081 tggactgcaa tgccattccc ggtgtgcccg aacgcaccta tgtcaactac ctgctggcct
     1141 ctcagcacga ccgcaacgtg gtgtatgccg ccttcaatca ccacaaatac ggcgatttca
     1201 aaccctattt ttacaaaagt tccgaccgcg gacgcacctg gaaggccatg agcaacaacc
     1261 tgcccgaacg cggctccgtt tattccatcg ccgaagacca cgttgacaaa gatctgctgt
     1321 ttgtgggtac cgagttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccc catcaaagac ggcttggtgt
     1561 atcacgagag ctacccgctg ggcctgcgcg gcaaggcatt ccaggggcac gcctacttca
     1621 cggcgcccaa cccgccggtg ggtgcagcct tcacctattt cgttaaagat gaggtgaaaa
     1681 cgctcaaaca aaagcgtcag gaggacgaaa aagaaaagat caaaaaaggc gaaccgatcc
     1741 gctatcctac ctacgaagaa ctcgaagcag agacccgcga agaagagcct tacctgctct
     1801 tcaccatccg cgacctgtcg ggcagcatcg tgcgcgaact caaaaccggc atcagcaagg
     1861 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccgc agccgatcgc gccgaactcg
     2101 tggccttcca gcgccaggct caagacctgc aacgcgtcgt ttccggcgca ggcagtatgc
     2161 tcggcgaaat caacaaccgc atgcagcaca tccgcaaagc catcttctcg gtgggtgcgc
     2221 ccaagtccga gttggtcaat gacttcaaag ccatcgaagc caagctctat gccatccgca
     2281 agaccatgta cggcgacaat attgccgcta ctttggacaa agacgatcct ttcacggtag
     2341 cctcccgcat aggctggctc accggcgaaa tgtggggcag cacttcggcg cccacgcaaa
     2401 cccaacgcga tgccttgcgc attgccgacg aggagtttaa accgctgttg gagcaaattc
     2461 gcnnnnnnnn nnnnnnngcg caacaacgac ctgcggatat tggaagaaaa attggaaaaa
     2521 gccggcgcac cctacacgcc cggccgcgat gtgaaatggg attgatttac aatgacttaa
     2581 tcatttaggg cggctgcccg ctcccgtatg gcctgcctct gctctctaaa aacgggcgga
     2641 ggcaggccgc caatgcgggc accgggtctt ccgctttcat gcagcatagc cacctctgcc
     2701 ctctctatgc cccatgctcc atgccctatg ctgcataccg ctacagcccc tgccgcaacg
     2761 cgcgcgctac cacttcttaa aaacaaaaaa caccgccagc accaaaaaca caaaacccac
     2821 caagtgattc catttgaaag tctccgtctt gaaaaaaatg agcgtgaagg ccatgaaaat
     2881 cagcagcgtc agcacctcct gaatcacctt caactcgatg agcgaaaacg gcccgccata
     2941 ctccttaaaa ccaatgcgat tggccggcac ctgaagcatg tattcaaaaa acgcaatgcc
     3001 ccaactgatg agaatgatcc agaagatgtg caattcttca aaccatttta tacctttgaa
     3061 tcggagatga ccataccagg ccaaggtcat aaatgcgttg gacaaaacga gcagcgcaat
     3121 ggtgtaccag gctttcatga tgagtggtta tttttaaaaa aatataagcg cgaagatacg
     3181 catggaacag ataatcgaag tttttgaacc tttgaaaagc ccctggcggg aaatggcact
     3241 gcccttgttg agcgcagttt tgctcgtcat tgcacaggta ctgcaactcc gaaacaaaaa
     3301 catagagcgc aaccgacgca tcgttttctc catgctgctg tttttcgggg cagtcattgc
     3361 gttgttaagc gccggcggta tcgccatcaa tgcgtacaag ttccagcgcg tcaccgtttc
     3421 cacttctgcc gtctccatcg gcaaaaaaga aattccgtac agcaatctgc ggggctacct
     3481 catccgcgaa gaccgcgagc agtcctgggt caattccgac attcagaaaa aagccgtgcg
     3541 cagtctggtg ctggaagaaa aaaacggcaa aacataccta ttgccggaag agtattacga
     3601 actttcgcac ctcattgcca ccctcgatgc cgcattctct caatggaagg aagtcggtag
     3661 gcagtaggcg gtgtgcggtg cgaagcgtag agcgttcatt cggtaggcgg tagccgcgcc
     3721 tgtggcgcgg ggtgctaagc gcgacttgca gcttgtagcc agcagcttgc agccagcagc
     3781 cgttccaacc cgaaacttaa attcttgaaa cgtcaaacca taaaaccatg attctattac
     3841 aaattgcagc aggcctcatc ttcatcctcg tgctgttcac gctattggcc acagccatac
     3901 aggagttgat tgccaattac atctccctgc ggggcgtcat gctgatgaaa accatacagc
     3961 acattttgga acccaacctc aaaggcattc gtggagaggt ttataattcc tttatcaaaa
     4021 gtttgtcttt caaacaatta tgccgccctt cgagcagctc ggcgtatggc cgtttcttag
     4081 actggctgga agaaaaaacc cgctatattt tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctctg ccgaaaattt tgccgaggtg
     4201 ctcaaaagat tggagtggaa acccgaatat ttagaaagca ttccgcccgg ccccctgcgc
     4261 gattatttag acaatctggg cgagcaggcc gaagacaaag tgcaggctcg cttgtctgat
     4321 ctcaaacgtt ggtacgacga ggtaatgaat gccactacca actggtaccg caggcaggct
     4381 caatttattc ttatttttat cggactggcc ttggcagcaa gttttaacnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnt cggcaaaacc ctctccaaag atgaaatcct gcgcacccaa
     4501 ttgtacagcg atatggaaaa atggatggcc gaacgcgccg acacctattt tgacgaaaac
     4561 gggcaggtgc gtcagggcgt tctggtgacc caaaatgata aaaacatcat tgtggtggac
     4621 cccaacctga aggacgacct gaaacaaagc gatgccgtac aacaacttat aggtaagaag
     4681 gcttcctttg ctaccgtggg agactggctc tacaacatcg ccaattggtt gctcaccgcc
     4741 attttcattt cattcggcgc gcctttctgg tttgacctgc tgagaaaatt ggtnnnnnnn
     4801 nnncgcgtgg ccaagccagt gtaacgtact gatttacaat agaaaatgga gtacattttg
     4861 gattgggcac tgcacggtct ctgctctccc gatttcgttc cttatcggga attcgtttcg
     4921 cttaaatgcc ctatgcttta aaaccaactt atgaacaacg aaaaaattct catcatcggc
     4981 gccaacgggc aaatcggaac cgtgctgaca caagccttgc gcgacttgta cggacaggat
     5041 caggtcatcg cttccgacct cgccgccgta ccaacgatgc ccgccggacc ttttgaaacc
     5101 ttggacgtgc tgaatgccgt gcggctgggc gagatcgttc ggcaatacgg catcgggcag
     5161 gtgtatcatc tggcnnnnnn nnnnnnnnna tcggccaaag gcgaacagca ccccaaatgg
     5221 gcctgggaaa tcaacatggg cggggtattc aatgtgctgg aagcggcgcg ggaatacaga
     5281 cttaaagtgt tctttcccag ctccatagca tcatttggcc cttttgccga aaggcagaat
     5341 accccccaac acggcccgct ggaacctacc accgtgtatg gcatcagcaa agtggccggc
     5401 gaactgtggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggaa gccccagcgg
     5641 agcaagtgaa gcaacgtacc agctacaacc tggccggcat gagttttaca cccgccgaaa
     5701 ttgccgccga aattcaaaag cactatcctg agtttcgcgt cagttatcag cccgatttcc
     5761 ggcaagccat agccgattcc tggcccgaaa gcattgacga tgccgatgcc cgcaacgact
     5821 ggggttgggc gccgcaattc gatctcgcct ccatgactgc cgagatgctg tctcgcctca
     5881 aagaaaaata tgcccgctaa ccatgtccta cgccttttcg ctccccttcc acattttccg
     5941 gctcaagttt cccaataaac acggcgatca tgttttgcgc cccgttgcag acattggttc
     6001 tgtgagcctc ggagaatcaa aacacctttt gacagagcgg tttgccgcct cttttcagca
     6061 ggaagtattg aacaaaggcc gggccggcga attgctcgac atgagtttgt atcaggattt
     6121 gaagcatacc gccattgcca cgccatttgc aggctcgtcc gacggcgtgt cttatcctgc
     6181 ttttgagatg gagtcggatg cgttcatcgg cgcccgaaac ggcacctggt ggggcatcgt
     6241 tccggcattg ggggtagagg ccgccgccga cagcgaagag ctgctcctgc aaagtctggc
     6301 cgacaacgtg cgcgtttatt ttgtgcgtac cggcaggctg cgcgctgtgc agggcatcgt
     6361 ttcggcgctg tggtatgagg aggtttcgtt ggaatcttcc gacatttcgc tgctctcgct
     6421 ggccccctcc gaagtggaaa atcaggaaac caatgccacc cggcaactgc tgagcgacgc
     6481 cgccacgccc ttgcccgcca cgcgcaaaga gttgttcggt atggagtacg aattggagca
     6541 ggttttgcgg gcttttcaaa acaatttcgg gcgctgtgtg ctattgacag gacctggcgg
     6601 ttgcggcaaa agcgccattg tagccgaagc cgcccggcaa tggaaaacca agcatcgggc
     6661 cggccagttt tgggaaacca ccgcttcgag gctcatcaaa gaactcaccc gcgataccgg
     6721 ctggcaatac aacctctctc acctctgccg cgaactgaat gccaacggcg actggctgta
     6781 tgtgcgctcc ctgatggaac tgtttgaggt ggggcgctat gtgggcaacg aggtgagcat
     6841 gggcgagttc ctgcgcaact acctgagccg cggcgaaata gcgctcattg cagaatgtac
     6901 cccggaggag ttgcagcgca tcgaactcat cagccccaat ttttcctctt ttttcagcaa
     6961 ggtggaagtc attcctccca aagaaaaagt gctggaaggc atcattcaga acagcgtgca
     7021 acaattgtcc gctgagcgca aaattgaatt cgacaaaacc gctgtgcgcg aggccattgc
     7081 cctgcacgcc cgcttcaacc cgtactccgg tatgcccggc aaagtgattc gttttttgga
     7141 aggcatcatc cgcgaaccct tgcgaaccgg cagtcagacc agccaacgcc gcatcaccgg
     7201 caaggatatt gtagagcggt tttgtgaaga aagcggcata cctgccctgc tgctgaatga
     7261 cgatctgcca ctggaacccg atcaaatact ggaacgcttc aacagcaatg ttttcgggca
     7321 accgcacgcc gccgctgccg tcacggatat gttggtcaaa ctaaaaacgc gcatctctaa
     7381 agagcgcaag cccatcgcat ccattttgtt tgcagggcct accggcgttg gaaaaacgga
     7441 attggccaaa gtgctggccg agcttgtttt cggcgaccgc aaccgcatga ttcgttttga
     7501 catgagcgag tactccggcc cggatgcggt gttccggctc accggcgccg gcgatgccga
     7561 cggattgctc acttccgccg tgcgcaagga gcctttctgc gtgctcttcg acgagattca
     7621 aaaggcatcc cccgacttct acgacatgct tttgcaaata ttggacgaag gccgtttgag
     7681 cgacaacagc ggcaaattgg tcaatttttg cagcgccatc atcattatga cctccaacat
     7741 cggggcggca ggaatgaagt cgaagcccat cggactgaag ccgggccggt cggcatccaa
     7801 cgtgcgcgag cattatcagg acgccgtgaa aaagtatttc aggccggagc ttgtcaatcg
     7861 cttcgacgac atcatccctt tcaacgcatt ggacgaggcc gccattcgat tcgtcgtgga
     7921 gcgggagttg aaactgttgc tgcaacgaga gggcatccga taccggcgca tgaacctgca
     7981 tattgacgag ggtgtcgccg attacttagg ccgccgcggg ttcgaccccc aatacggagc
     8041 gcgttacctg caacgggcca tgcgcgaaat gctgctgttg cccttgtcga aatcactggc
     8101 ggagcaagac cccacagacc acctcgaagt atctgtctat atggatgctc agggctaccg
     8161 accggcggta aaggttgaat cggacccgct ttcgctggaa ttgctcattg aggaattgca
     8221 ccacgccaac atcaccaacc acacttctac cctgcgaaga acgatttacg cggtagagga
     8281 cggctttttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatga tcgccggagc
     8341 cacccaaccg gattttggaa agaccatgag cgcgtgcggg aatacaacgc tttgtcgaag
     8401 tttacagagc gcatgaacgg ccttaaaacc gaaatcgaaa tgctggaaat gaacgcctcc
     8461 gtcagctttc tggggcaggc ttccgacgat gtcaggctcc ccacccagct caccaactgg
     8521 acttcagaag cgcaggtgtt taaaaaagac ctcgtagccc acgtcaaccg cgagtacaac
     8581 tcctgtgtgc tgggtttgta cggtgttgat ctggaaaaaa tttacaacat gtaccgcgtc
     8641 attgtccgca ggctcggcat tcagatgagc ggagtatggc ccgtctggca cagaaattcc
     8701 tactatgaag aattggtgtc cgaccccgaa aacccggagg taaaagtaaa acgcgaggaa
     8761 tattattata cagaagaaga gtggtttaca ggcacttccg gcgactaccg ccagcctaca
     8821 gccgaaaaaa gcggcgacaa attggtgggt ctggagttca aaattgacgg ccctgtggcc
     8881 tacctgctga tgtcgggcga aagcggtttt caacattggc tgctccccgg ccataccgaa
     8941 ccgacgccgt actgggtgca ggtcatgccc tttgaacagc acgcaacccc gaagcgcgtg
     9001 catcgtttgg acttcttcac tgccaaaccg aggagaatca tggctcccgg aacgtactcc
     9061 gacaaaaact acaatattga agcggctaca gaaagtgagt ttttggatgc cgtcgctcaa
     9121 cactgtcagg catacatgat acaggaagtg aacctggccg tgatgtaaca aatccctacc
     9181 tttgcggctc aatcattttt tcagatgaca cgcaaagcca aagccgccgc cataacagca
     9241 atattggaac gtctctaccc ggaaacgccc attccactgc aacacgaaga cgcctacacc
     9301 ctgctcgtgg ccgtactgct gtctgcccaa tgcaccgacg agcgcgtcaa taaagtcacg
     9361 ccgcatctct tcgcacttgc cgacaatccg caggaaatga gccagttacc ggtgcaaacc
     9421 atccacgaca tcatacggcc ctgcgggctg gcgccgcgca aatcgcaggc catttccgac
     9481 ctgtcgaaaa tcctgataga acagcacaat ggccgggtgc ccgacaactt cgaggcctta
     9541 gaggcgctgc cgggcgtggg acacaaaacc gcctcggtgg tgatgtcgca ggcattcggc
     9601 gtgccggctt ttccggtgga cacgcacata catcgcttag cctggcgctg gggactgagc
     9661 agcggcaaaa atgtggagca aaccgaggcc gatctcaaga agctttttcc nnnnnnnnnn
     9721 atcaagctgg aacaaactgc acctgcaaat cattttcttc ggcagggaat actgcccggc
     9781 gcgcggccac gatccgaatg tctgccctgt ttgcagcctt tatggaatca aggaactctt
     9841 caagccaaat ctataataac catgttcaca ggcatcatag aaaccgccgg gcgggtagtc
     9901 gccatgcaac aagaaggaag caacgtacat ttcaccattg cttccgatat ttcttcacaa
     9961 ctcaaagtag atcaaagcct tgcgcacaac ggcgtgtgcc tcaccgtcac cgctttgggc
    10021 gagggcacgc acaccgtcac cgccatagaa gaaaccctgc aacgcagcaa cctcggccgg
    10081 ctccgccccg gcgacgccct caacctcgaa cgggccatgc gctccgacgg gcgtttggac
    10141 ggccacattg tgcagggcca tgtggacacg accggcatct gcaccggcgt ggaggaactg
    10201 gagggcagtt ggtacttcca tttcgagtat gcgcccaatc ccgatcattt gttggtggac
    10261 aaaggctnnn nnnnnnngtc aacggcgtga gcctcacggt ggtgaatcct accgaaaaaa
    10321 ccttctcggt ggccatcatt ccctacactt atgagcacac caatttcaag gccatccgct
    10381 ccggcgatac ggtcaactta gagttcgatg ttatcggcaa gtacatcgcc aggtatgtgc
    10441 ggctgtatat ggggtaataa ggtacaactc agagattgga tttgcgaggc tgcccgcatc
    10501 agtccggatg accttttata atcacttttt accaatgagg gtgtagccca gccccaactg
    10561 gaaagccaag ttgcggcctg tagtttccaa tggctcgccg ttgacgtcgg tgaattgcgg
    10621 atcgggaacg aatttgtggt cggtcatcag gccgaactgc acattgccgt gcaatgagaa
    10681 gcgacgaaca gcatacttta agccgccggt gaggccgaaa tcaacatcat tgtataagaa
    10741 ttctgcggcc caatcaggca ccttattcca gacgccatcc cccatattat tatacgctcg
    10801 gagaatatga ctcacataag cgccggtttg aagggaaaaa tcaggcgtga gataaaagcc
    10861 tattctcgga ttcaactcta tggtttgaaa gcgatccata gatggttctg taacgnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc caatgtgata gccgccctga
    11041 aatattcgag gtgttggctc ctgtgagggg tcgaataaaa aaggggcatt agaggacaga
    11101 atatacctgc tttgttgtat gccgccctgc agggcaaggc tgaattgtgc agataggctg
    11161 ccggaaaaca tggttgcaaa ggcaaggatc aggagcggct ttttcattga gaagtttgtt
    11221 ttggttccct ccgagaacga gccttgtaat gtatagtatg caggaagata gttaattaac
    11281 aaggggtcat tgcccccgtt gcgccaggga tagaaacgaa atggcggcgg tcatcgcaga
    11341 gctaacagcc tgataacatg ggcagagctg ttagctctct cctcgagccg ccgccatgca
    11401 gtggatagcc cggccacgcc cgcccgatgg ctccctcctt tggagggtcg gggaggcagg
    11461 tagaggcgtg gggcgccctg aaaataccgc ctcacttccc cttcaccgga ggcagcgcat
    11521 atcgtttgtt ccaccaatag gagacggaac cgatggcgag gttgagcagg ccgaggaggc
    11581 tttcgcgggg cttgtcgtag agcaggaagg ccagcatcca gacgctgaaa aggaggaaga
    11641 tgatttgcgc ccaggggtag aacggggagg caaaaggcgg gcgacgacct ttgtctttgc
    11701 gacgcaggaa gaacaacccg gccactgctg cggtggtgga gaggtgcagc acgaagccgc
    11761 tgtaagtgag tacttgctcg aaggagccgc tgagcagcag gaaaatgctg acggcggcct
    11821 gaaaccagat ggcattgacg ggtacgccgt ggcggttgtc gcgggccaaa aaataccata
    11881 ccggatagtc ttcggccatg gcgcggctca cgcgaggacc cacccatacc atagcgctga
    11941 tgctggacac caagagcagc ccgatgagca ggctgacggt ttggccgccc gatgcaccga
    12001 acaccgaaac ggcggctatc tgggccactt ctattttgcc ggccaatgcc tcgcggcccg
    12061 cctgatagag gaagcccatt tgcaacagca catacaacac gctgaccaac aatgcgccac
    12121 ccaccaaagc gcggggcaga ttgcgggcgg gttggcgtac ctcgccggtg atgtaggccg
    12181 ccgcattcca gccggaatag gcgtacacca caaatactaa tgcgacggcg tagggcactg
    12241 tgccgagttc gaacagccag gggccgctcc atgccaggcc gttgatcggc gccgagtgga
    12301 cgaaacaagc cagcgccaga aagaccatca aggccagttt gaggatggtg aagatgtttt
    12361 gaaaacggct gctctgctcg acatggaacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnngggcat ctgtgccgac cacttcggag aggtaggcgc ccatggccat
    12481 ggccgacagc gcaatggagg ccgtaaaacc tacggtgagg gatatccagc cggagagata
    12541 gccggccagc gggtggtaaa tttgggaaag gtaatagtat tcgccgcccg aacggggcat
    12601 gcgcgcgccc aactcggcgt agctgacggc gcccagcagc gcaataacgc cgccgaaaac
    12661 ccagagcgac aaaatagccc aggtgctgtg gatgtgctgc aattgcaagc ccagcgtggt
    12721 aaacgcgccg gtgcctacca tgttggctac tacgatagag ccggcgctga cccagcctat
    12781 tttgcgatgt gttgtcgggt gcatgtggta cggcggnnnn nnnnnnnncc cccggagagc
    12841 ataaacggca gcttggtgat cagatcgggt gcaagcacaa ggcttttaac gcctgattaa
    12901 cgttgcggtt tatctctttt ccggttcctc cgcattttgt gctccatgca attgcaccgg
    12961 agtgtgctgt ttgcggagcc gcatgttgag gaattccaca aacaaagaga aggaaatggc
    13021 aaaatacagg tagcctttgg gtacggcgcc tacctcggaa cccgcgatgg acaaatgagc
    13081 taggtgcgcg ccctcggcaa tgagcatgaa gccgatgaga atgagaaatg ccnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgttaatc caactgcggt
    13261 gaggatggag tcgaatgaaa agacgatatt gatgacggtg atctgcacaa tgacgttgct
    13321 cagcgtcttt ttcaatttgc cgtcttcgct ctgtccctgc acggtatccg ggccttcgag
    13381 tttgtggcga atttcggtgg tgcttttgta caggaggaac aggccgccgg cgatgaggat
    13441 gaggctttgg cccgaaaacc ctgctttgat ccagttttga tcgaagtgaa tccagggttc
    13501 ttccatagcg atgagccagg aaaccccgaa cagcaaagcg atgcgcatca ccatggccaa
    13561 cagcaaaccg at
//
LOCUS       DAORLF010000060        13490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2443_length_13490_cov_1.744622, whole genome shotgun sequence.
ACCESSION   DAORLF010000060 DAORLF010000000
VERSION     DAORLF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2443_length_13490_cov_1.744622"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK198_07190"
     CDS             complement(<1..>214)
                     /locus_tag="PK198_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38555.1"
                     /translation="FYLAQLAPDGKIYINSNNGVRYLHVINQPDSLGLACDVCQHCVE
                     LPSINALSLPNFPNYRLHHLEGSPCDT"
     assembly_gap    215..336
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<337..1377)
                     /locus_tag="PK198_07195"
     CDS             complement(<337..1377)
                     /locus_tag="PK198_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38556.1"
                     /translation="MKTALLLSPSKIFGAFFTAPFAAQTDGTIAIYRGIFCGSMPQEI
                     LLFLIPRIMRILLVFHCMFFLILTAQGQKHDYVWLLGQNSQTNLIYSGTVVDFNTTPP
                     DIYYEFRSMFFSQANASMCDTAGNLLFYTNGIYVANALNEPMENGMGLSPGTHSQQNQ
                     NYGYILTQGAMILPTPESGGLYHLFHMDRELNSQQTSTESRNFYFTLIDMDMNNYLGG
                     VVLKNQKIIEGLFDIGRITASKHANGRDWWLLTRKYDTNEYYRILFDIEGLVVQGVQA
                     VGSPIPSNTIGQSVFSPGGTKYACVHVYGLANPIYISIYDFDRCNGTLSNPIQFTYTD
                     TASCAGVAISPNN"
     gene            complement(1648..>1835)
                     /locus_tag="PK198_07200"
     CDS             complement(1648..>1835)
                     /locus_tag="PK198_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF38557.1"
                     /translation="NNELIIFNRWNDQVFRAAPYLNNWRGVNQSGQELPQGTYYYILR
                     LNIAEGEIIMGDITIVK"
     assembly_gap    1836..1869
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1870..>2346)
                     /locus_tag="PK198_07205"
     CDS             complement(<1870..>2346)
                     /locus_tag="PK198_07205"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF38558.1"
                     /translation="VVVSPNTAQTNVTNAQPGDNRFVWTLSTAACQSYSADTITVNVQ
                     RGPVAGNDMLALASGVFSGSVNIAANDQLFGAPGFTITVLTQPEFGTVDSIVNGTLYY
                     SVPRGAFGQTQVRYQICNTQCPDLCAEAFVQIDVERTTIVYNQPNGITPNGDGLNEE"
     assembly_gap    2347..2356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2357..>3479)
                     /locus_tag="PK198_07210"
     CDS             complement(<2357..>3479)
                     /locus_tag="PK198_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38559.1"
                     /translation="GPSIERFITCDSTTITAHIAYTPFNPDFNIVWSITSPAGNIVSI
                     SPDSLTIVVSEQGTYTVRVTNPANGCVGENEVVVGLDRDFPIATISSPDTVVSCITTV
                     VSLNGNGSSAGPEFSYQWNAVAGGPLNQIAPLQVTTTAAGTYELVVRDSSNGCVSRDT
                     IGVTVNQAAPTATIADPQALTCTLTQVSLNGNGSSNGPNFTITWNGLDGGTVTPTGNP
                     LQVNATTGGRYELFIRNNQNGCEARDTVTVTANNTPPVANAGADQTINCNGTDVTLDG
                     SGSTPAGSLGYLWSAISGGGPINGTTIATPVVNTAGTYQLIVTDNTNGCRDTATVMVT
                     IQNNLPSAMAGEDFGVCGGDIMLDAMLPSGVTGMWRSLGS"
     assembly_gap    3480..3507
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3508..>4988)
                     /locus_tag="PK198_07215"
     CDS             complement(<3508..>4988)
                     /locus_tag="PK198_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38560.1"
                     /translation="DLAFTCNDDEVVINATVTNSTSFEVLWTPLDGGSLFFGSEATLN
                     AIATTPGTFQVFVRDLFNLCTAIDTVVIADSTALPNFVLAMPDSLSCDSTQIRIFAEA
                     PSGGNYDFRWYSNGVELSEQTSTLTVTTPGFYALEVLNVFTGCSRIDSVEVFIDTLAP
                     IVGVVPPPTWTCLFNTIPMTGNIVTGSVPGMLFNWTNSSGDTTGIIGHLLQNPVMTLP
                     GDYTFRATNPNNGCFSEATVTIQADSIPPTANAGMDQTIGCAGESALLRGTGVSGVNG
                     VSYAWFGPDGMQVATTDTVTVSVLGNYVLRVTDQVNGCSATDTLSVGASNDVPTVAIA
                     SDTLRLDCNTNRVTIPGSVTFNGAFNFQWHSAQLVGGTEMSQTPQTETAGTYILTATN
                     PANQCTASDSVVVIVDREPPVAVSGDDFTLTCVAVAYSITSTGSTPGANLRWYLGSVG
                     SPVLGTDPALIVNAPGTYILEVVNPVNGCTATDTTIISQDGTPP"
     assembly_gap    4989..5015
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5016..>8123)
                     /locus_tag="PK198_07220"
     CDS             complement(<5016..>8123)
                     /locus_tag="PK198_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38561.1"
                     /translation="FSNITGTSYQIRWDPTILQYVNLTMPTLPGFTVGTNVDASAAAS
                     GVLTISWSVANCNTPGATGVTLDDCFGQCRPSIFKLCFTMLSDTYGESTNIGVGPNPY
                     VTKDNSSCLNVGLINNDGIVTNCVRPVELIASQEQGNSGDLICVDYRLTGFDDLNSMQ
                     FTINYDPAVLSFQNVVIPGNLPNFSQANMGLPPNLPEGTITVSWSYTPPNNAGITLLD
                     STLLFQVCYRIVGPCESASMISFGETPTPIEFGNTIVQNFDIMVLPRNGRVTASDCDP
                     TGMPINISCGPARNLGETFCVQVTSQNFFNVAAFNYLIEWNPALLEFVNVSNVTTNGI
                     ANFNVANSFSTANVQAGVLGVNWVRTGNNNAFIPNGTVHYEICFRVIGVGGNSPVTAG
                     GPLPLVQVNNLNAPNIGINPSNCEVVINQPTGITLTVSDASAQLNQQVCVDITAANFT
                     NITRLRYSLNWDPLHLQFVSINALNLPGVVPLVNFGLQGQSSGTVTFDWSTMTPVTRP
                     DGTALFRVCFVVVGQPGDCEAVQINDDPLTREAVTSFSNGNNIGITSQDGEVCSLFPE
                     GFFMTVGNINTAWRDTVCVPFRVASFDNITEAHFEVSFNPSNLQFTGVNNLASLPGLS
                     VSSFTTTSAGLGIIEFDWVNFAGSMLPDSTILFELCFAAMGEADGACHKIDIIRDPAP
                     TVLTTAGIGSLVWRNGEVCVADKLIIVNATIIPVSCPGGRDGRIELEVIGGKPPYGNT
                     WETEPLQFMPLLGRNLAEGQIIVTTRDNNIPATVRVDTFYVPLAADAPTVDAGPDRPF
                     PCDGSPVLGIQGQASQGANYGYKWTTLGGMLAQPDTNTLALALQQGIYILSVTNRASG
                     CVVSDTVNISQPNLPIADAGEIQLTTCTSDTVTLNGSMSSTGDTVTYLWAALNGGSIV
                     AGDQALSSPRVVGAGTYALEVRYQLSGCIARDTVVVEDGAIAPLAVGGSDPVIGCSGS
                     IILDGSSSSGTNAIDIRWTTLDNTVLSPTATYETSTPGSYLLVVTDLVNGCSATDTVT
                     VSLTAD"
     assembly_gap    8124..8211
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8296..9246)
                     /locus_tag="PK198_07225"
     CDS             complement(8296..9246)
                     /locus_tag="PK198_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF38562.1"
                     /translation="MNVLRTLLPVALLLCSIASHGQQLSLFTQYRENAVIINPAAMES
                     DFLTFGNNMTFGASYRAQWTGIKSAPRTQTLRMSFVNKRYAGVTPMFGGHIVNDQTGP
                     TGFTGIYGRIGGVITGDAEYGGLALALTAGAVQYRVKASELVLRDDGDVLGSADQTRI
                     FPDVGLGLYYYQMVGDGNFFYTGLSMPQALGLDLTFKNENGQFFTKRVQHIYGSIGFY
                     KFVGDDGFIEPSVLFKYAPNAPINVDVNLRYQIPGSLWVGAGASSAKAVHLETGFLLG
                     QNLGFDNIFRIGYGFDYSFSSFGPSIGATHEINLTLSLDR"
     gene            complement(9496..9843)
                     /locus_tag="PK198_07230"
     CDS             complement(9496..9843)
                     /locus_tag="PK198_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRF38563.1"
                     /translation="MSKKNKNRDGVVFSTDPDFAYRYEEQEEAELLPAQQQKLRVSLD
                     RKQRGGKEVTLVTGFVGPQSELEALGKMLKTKCGVGGAAKDGEIMVQGDHRERVLQIL
                     LQAGYSGSKRSGG"
     gene            complement(9937..10869)
                     /locus_tag="PK198_07235"
     CDS             complement(9937..10869)
                     /locus_tag="PK198_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF38564.1"
                     /translation="MKIIHTESIAACCICLAVVVSACMNEAGFSSADKAVSRKDGMVL
                     VPSGMLQMGGDNEQADANEFPKHDVEIHAFWMDEHEVTNAQFAEFVKATGYVTVAERP
                     IVWEEMQKELPPGTPKPADSLLQPGALVFRASKHPVPLDDPGRWWAWTLGAHWKQPEG
                     PGSSIEDRMNHPVVQVAWEDATAYCKWAGKRLPTEAEWEWAARGGLSNMVYPWGNEPV
                     EAGQAKANFWQGMFPYLNEMGYSLRTQRYRYTAWYSCKYTEGQSPLDGRLLAVELYDY
                     LSEPQETVNLAGKLEVEAELREKLRTYFQNNSNK"
     gene            complement(11011..11157)
                     /locus_tag="PK198_07240"
     CDS             complement(11011..11157)
                     /locus_tag="PK198_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38565.1"
                     /translation="MSERNHTRIKELTAAGDMERLAAHLKVHQRLLEIRNELALELGT
                     VVLK"
     gene            complement(11094..>11539)
                     /locus_tag="PK198_07245"
     CDS             complement(11094..>11539)
                     /locus_tag="PK198_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38566.1"
                     /translation="DIARILIAFSHEEFDKDDHLSVAEYIISNIEDVLDEFDNPLYAK
                     VAREVLACLANRRSQLNTNYFLHHSDPEISLLAVNLLSEPYEYSENWANRWEIYLRMQ
                     KMPDDNFQEDSLQALKRFSCAKSCACRSAITPASKNSPLPETWSA"
     assembly_gap    11540..11579
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11580..12275)
                     /locus_tag="PK198_07250"
     CDS             complement(<11580..12275)
                     /locus_tag="PK198_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRF38567.1"
                     /translation="MLYGAYQARKAIREKDECILVEGYTDVISLHQAGIENVVASSGT
                     ALTVEQIGLIKRNTNNIKILYDGDPAGIKAALRGLDLVLEQDLNVRIVLLPDREDPDS
                     YLARVGATAFTEYIARQAKDFILFKADLLMQEAGSDPVKRSGVVRDIVTSIAKIPDPV
                     KRSLFVQECARVAQVDEAILMSETNKLVLAQMQKRHAPGREGERSPADVSSDTDAVPV
                     PAGQAPEQEAARKA"
     gene            complement(12284..13015)
                     /locus_tag="PK198_07255"
     CDS             complement(12284..13015)
                     /locus_tag="PK198_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRF38568.1"
                     /translation="MITQKSVQEILDTVKIEDVVQDFVSLRRRGTNLIGLCPFHHEKT
                     PSFNVSPARNIFKCFGCGKGGNAVQFLMELERFTFPEALKYIAKKYGIQIEEIESSRE
                     AAEERRVADSLYIINQYALEFFQNQLVNTDRGRSVGLSYFRERGFREETIQKFGLGYA
                     PEKRDQFLTTATAAGYDQQMLFNLGLCSPPDASRNLPPRDFFRDRVIFPIHGMSGKVI
                     AFAGRIMAKDVKAPKYINSPEPTST"
     gene            complement(13083..>13490)
                     /locus_tag="PK198_07260"
     CDS             complement(13083..>13490)
                     /locus_tag="PK198_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HRF38569.1"
                     /translation="VRWVRRREVVSSVTLTGPESQLAGVGEWPTDSVVLEQLKKTVDQ
                     TVDLRPPPKGVYISADKVKVKISVEQLSEKTFWVPVTVLHDADSVRVFPKNIQITCTL
                     GLSRYDSTIAEDFEITADLRGAALPSATPHCCN"
BASE COUNT         3047 a         3420 c         3548 g         3126 t
ORIGIN      
        1 gggtgtcgca gggactgccc tccaagtggt ggaggcggta gttggggaag ttgggaaggg
       61 agagtgcgtt tatagacggc aactcgacac aatgctggca tacatcacag gccaaaccta
      121 aagagtccgg ctgattaatg acatgcaggt accttacccc gttgttgctg ttgatataaa
      181 tcttgccgtc cggagccaat tgggcgaggt agaannnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtta ttgggggaga tcgcaacacc
      361 tgcacaactg gccgtatccg tataagtgaa ctgtatgggg ttactcaacg tcccgttgca
      421 acggtcgaag tcgtagatac tgatatatat ggggttggca agaccatata catgaacaca
      481 ggcgtatttt gtgccgccgg gcgaaaaaac agattgacca attgtatttg aggggatagg
      541 gcttcctact gcctgtactc cctgcacaac caacccttct atatcaaaga gtattctata
      601 atattcattg gtatcatatt tcctcgttag cagccaccag tcgcggccat tggcgtgctt
      661 agaagctgtt attcttccaa tatcaaataa gccttctatt attttttgat ttttcagaac
      721 gactcctcct aaataattat tcatgtccat atcaattaat gtgaagtaaa aattgcgcga
      781 ctctgtactt gtttgttgcg aattcagttc tcgatccatg tgaaacaggt gatacagacc
      841 tccactttca ggagtaggga gtatcatagc tccttgagtt aagatatatc catagttttg
      901 attttgttga gaatgagtac ccggacttaa ccccatccca ttttccatag gctcattcaa
      961 cgcattggcg acataaatac cattggtgta aaaaagcaaa ttgccggcag tatcgcacat
     1021 actcgcattg gcttggctaa aaaacatact tctgaattca taataaatat cgggaggcgt
     1081 cgtattgaaa tcaactacgg ttccagagta tataagattg gtctgactgt tctgtcccag
     1141 taaccaaaca tagtcgtgct tttgtccttg agcagtaagt attaaaaaaa acatgcaatg
     1201 aaatacgagt agaattctca ttatccgagg tattaaaaac aggagtatct cctgcggcat
     1261 gctgccacag aaaatacccc tgtagattgc aattgttcca tccgtttggg ctgcaaaggg
     1321 cgcagtgaaa aacgctccaa aaatcttgga aggggataga aggagggcag ttttcatggc
     1381 aaattatttt atggtaaaaa gtaacgaaaa tacaggggaa cgcggctttt ccgcagggta
     1441 aaacttaata gcacaggcaa atgtaatgac cagagtggga aaagcgaaag aaatgggggt
     1501 gtttttttta caaaaatgtc agggtatagt aaaggggcga cttggcaagt ttcagcccgg
     1561 cagtcacccc ttcactaagc cgaaaaaagg ccctcccgat gatttcgaga gagccttttt
     1621 cttgttatat atctgaaact gaatcgctta tttcactatc gtgatatcgc ccataatgat
     1681 ctcgccctca gcgatgttca ggcgcaggat gtaatagtat gtaccctgcg gcaattcctg
     1741 ccccgactgg ttgacgccgc gccagttgtt gagataggga gcagcgcgga atacctgatc
     1801 gttccaacgg ttgaaaataa tcaactcgtt gttggnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnc tcctcattga ggccgtctcc gttgggcgtg atgccgttgg gctggttgta
     1921 aacgatggtg gtgcgctcca cgtctatttg cacaaaagct tcggcgcaca aatccgggca
     1981 ctgcgtattg caaatctgat aacgaacctg cgtctgcccg aatgctccgc gcggcacgct
     2041 gtaatacaag gtgccgttga cgatgctgtc caccgtacca aattccggtt gggtcagaac
     2101 ggtgatagta aagccgggag cgccgaaaag ctggtcgttg gctgcaatgt tcacagagcc
     2161 gctgaacacg cccgaagcca gcgccaacat gtcgttgcct gctaccggac cccgctgcac
     2221 atttacagta atcgtgtctg cgctgtagct ctggcaagct gccgtagaga gcgtccacac
     2281 aaagcggttg tcgccgggtt gggcattggt cacgttggtt tgcgccgtat tggggctgac
     2341 cactacnnnn nnnnnngaac ccaagctgcg ccacataccc gtcaccccgc ttggcaacat
     2401 ggcatccagc atgatgtcgc cgccgcacac gccgaagtct tctccggcca tggcgctggg
     2461 caggttgttt tgtatcgtta ccattacggt agcggtatcg cggcaaccat tggtattgtc
     2521 ggtcacaatc aactgataag tgccggccgt attcactacc ggcgtggcaa tggtggtacc
     2581 gttgatgggg ccgccgccgc tgatcgcgct ccacaaataa cctaagctgc ccgccggcgt
     2641 tgaaccggat ccatccagcg tcacatcggt gccgttgcag ttgatcgtct ggtcggcgcc
     2701 ggcattggct acgggaggtg tattgttggc cgttaccgtt acggtatctc tggcttcgca
     2761 gccgttttgg ttgtttctga tgaataattc gtagcgaccg cccgtagtgg cgttcacttg
     2821 cagcgggttg cctgtcggcg tcaccgtgcc tccatcaagg ccgttccagg tgatggtgaa
     2881 attggggccg ttggaagagc cgttgccatt cagggaaacc tgggtgagcg tacaggtcag
     2941 cgcttgcgga tcggcgatgg tggccgtagg agcagcctga ttcaccgtca cgccaatggt
     3001 atcgcggctg acgcaaccgt tggaactgtc tctcaccacc aattcatagg tacccgcagc
     3061 ggtggtcgtc acctgcaagg gcgcaatttg attgagtggg ccgcctgcaa cagcattcca
     3121 ttggtagcta aactccggcc cggccgatga gccgtttcca ttgaggctca ctaccgtagt
     3181 aatacaactc acaaccgtat cggggctgga aatcgtggcg atgggaaaat cgcgatccaa
     3241 accaaccacc acttcatttt cacccacaca tccgttggcc ggattggtga cacgcaccgt
     3301 gtaagtgccc tgctcgctga ccacaatggt aaggctgtcg ggagaaatgg aaacgatatt
     3361 gccggcagga ctggtgatgc tccaaacgat gttgaaatca ggattgaagg gcgtgtaagc
     3421 aatgtgcgcc gtgatggttg tactgtcgca agtgatgaag cgttcgatgg acgggccgan
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnngga ggagtgccgt cctgtgaaat gatcgttgta
     3541 tcggtagccg tacagccatt gactggattg acgacctcca agatgtaagt gccaggcgca
     3601 ttcacgatga gcgcagggtc cgttccgagt accggcgatc ccacactacc gaggtaccat
     3661 ctgaggttgg cgccgggcgt agaaccggtt gacgtaatgg aatacgcaac agccacgcag
     3721 gtgagcgtaa agtcatcgcc ggaaacggca accggcggct ctctgtccac aatcacaact
     3781 acagaatctg aggcggtaca ttggttggcc gggttggtgg ccgtgaggat gtaagtgccg
     3841 gccgtttccg tttgcggagt ctgactcatt tcagtgcctc ccaccaactg agcactgtgc
     3901 cattgaaagt tgaaagcgcc gttgaaagtt accgatcccg gaatggtaac ccggttggta
     3961 ttacaatcta agcgcaaggt atccgatgca atggccacag tgggcacgtc attactggcg
     4021 ccaaccgaca aagtatccgt agcagaacat ccgttgacct gatcggtcac tcgcagtaca
     4081 taattaccta aaacagaaac cgttactgtg tcggtggttg ccacttgcat accgtcaggg
     4141 ccgaaccatg cgtacgaaac tccattgact cccgacaccc cggttcctct cagcaaggct
     4201 gattctccgg cacagccaat ggtctgatcc atgcctgcat tggcggttgg cggtatggag
     4261 tcggcttgta tcgtcacggt tgcctcgctg aagcaaccat tgttcggatt ggtagcgcgg
     4321 aaggtatagt cgcccggcag cgtcatcacc ggattttgca gcaaatgccc gatgatgccg
     4381 gtagtgtcgc cggagctgtt ggtccagttg aacaacatac ccggtacgct tcccgtaaca
     4441 atattacccg tcatggggat ggtattgaaa aggcaagtcc aggttggagg cgggacaaca
     4501 cccacgatgg gcgccagtgt gtctatgaat acttcgaccg aatcaatgcg ggagcagccg
     4561 gtgaacacat tcaatacttc caatgcgtag aatccgggcg tcgttacggt cagcgtcgag
     4621 gtttgttcgc tcagttctac cccgttggag taccagcgaa aatcgtaatt accgccggag
     4681 ggcgcttctg caaaaatccg aatctgcgta gaatcgcaac tcagagaatc cggcatagcc
     4741 aacacgaagt tgggcagcgc cgtactgtct gcaatcacaa cggtatctat tgccgtacaa
     4801 aggttaaaca ggtctctgac aaaaacctga aaagtgcccg gcgtggtggc tatggcattg
     4861 agcgtcgctt ccgacccaaa aaacaaagag ccgccgtcta aaggcgtcca caatacttca
     4921 aaagaggtgg agttggtcac tgtggcgttg atgactactt cgtcgtcatt acaggtgaaa
     4981 gccagatcnn nnnnnnnnnn nnnnnnnnnn nnnnnatccg ccgtcaggct taccgtaacg
     5041 gtatctgtgg cagaacaacc attgacaagg tcggtaacga ccagcaaata gctgcccggt
     5101 gtgctcgttt cgtaggtggc tgtcggcgaa agcacggtat tgtctaatgt cgtccatcga
     5161 atatcaatcg cattggtacc cgaagaagag gagccgtcca atatgatgct cccgctacac
     5221 ccaataacag ggtctgaacc accgactgcc aaaggcgcaa tggcgccgtc ttctaccaca
     5281 accgtatctc tggcaataca tccactcagt tgatagcgca cttcaagcgc gtaagttccg
     5341 gcgcccacta cgcgcggaga tgagagcgcc tgatctccgg caacgatgct gcctccgttg
     5401 agggccgccc agaggtaagt tacagtgtcg ccggtgctgc tcatgctgcc gttgagagta
     5461 acggtatccg aagtacaggt tgtcaattgt atttctccgg catcggcaat gggcaggttg
     5521 ggctggctga tgttgaccgt gtcgctcact acgcaaccgc tggctctgtt ggtgacggac
     5581 aagatgtaga tgccctgttg cagcgccaga gcaagcgtat tggtatccgg ctgagccaac
     5641 atgcccccca gtgtggtcca tttgtatcca taattggctc cctggctggc ctggccttgt
     5701 atgcccaaca ccggcgatcc gtcgcagggg aaggggcgat ccgggccagc atcaaccgtc
     5761 ggtgcatcgg cagccagcgg cacatagaac gtatctaccc gcaccgtggc cggaatattg
     5821 ttgtcgcgtg tggtcacaat gatctgtcct tcagcgagat ttcggccaag cagcggcata
     5881 aattgcagcg gctcggtctc ccaggtattg ccgtaaggcg gctttccgcc aatgacctcc
     5941 agttcaatac gtccgtcacg gccgccggga cagctcaccg ggatgatggt agcattcacg
     6001 atgatgagct tatcggccac acaaacttcc ccgttgcgcc acaccaaact gccgattcca
     6061 gcggtggtaa gtacagtcgg cgcaggatcg cgtatgatgt caattttgtg acaggcgccg
     6121 tcggcttctc ccatggccgc aaaacacagt tcaaacaaaa tcgtgctgtc agggagcata
     6181 ctgcctgcaa agttgaccca atcaaattct atgataccca ggccggccga cgtggtggtg
     6241 aaggaggaaa cgctgaggcc cggcaatgaa gctaagttgt tgactcctgt aaattgcaga
     6301 ttgcttggat tgaaactcac ttcaaaatgc gcctccgtga tgttgtcgaa actggccacc
     6361 cggaaaggca cgcaaacggt atcgcgccaa gctgtattga tgtttccaac cgtcataaaa
     6421 aatccttccg ggaacaggga gcacacttcg ccatcctgag aggtgattcc gatgttgttg
     6481 ccgttggaga aagacgtaac agcctcccgc gtgagcggat cgtcgttgat ttgcacggct
     6541 tcgcaatcgc cgggctggcc cactaccaca aaacaaaccc tgaacaaagc cgtgccgtcg
     6601 gggcgcgtca cgggcgtcat ggtactccag tcaaatgtaa cggtaccgga agactgcccc
     6661 tgcaaaccaa agttgaccaa aggcactacg cccggcaggt tgagggcatt gatggagaca
     6721 aattgaagat ggaggggatc ccaattgagg gaatatctca gtcgggtgat gttggtaaaa
     6781 ttggcggcgg tgatgtccac gcatacctgc tgattgagtt gcgcggaggc gtcgctcacc
     6841 gtgagtgtaa tgccggtggg ctggttgatg accacttcgc agttgctggg attgatgccg
     6901 atgttgggcg cattgaggtt gttcacctgc actaacggca gcggtccgcc ggcggtcacc
     6961 gggctattgc cgcccacgcc tatcacccgg aagcatattt cgtaatgcac ggtgccgttg
     7021 ggaataaagg cgttgttatt accggtgcgt acccaattga cgcccaaaac gccggcctgc
     7081 acattggccg tagaaaagct attggccacg ttgaagttgg caatgccgtt ggtggtcacg
     7141 ttggatacgt tgacaaactc aagcagagcg ggattccatt ctatcaggta gttgaaggcc
     7201 gccacgttga agaaattctg agaggtgacc tgcacacaaa aagtttcgcc gaggttgcgc
     7261 gccgggccgc aactgatgtt gatgggcatg ccggtaggat cgcaatcaga ggccgtaacg
     7321 cgaccattgc gaggcaaaac catgatgtcg aaattttgca caatggtatt gccgaactct
     7381 atgggagttg gagtttctcc aaatgaaatc atcgaagccg actcgcaagg accgacgatt
     7441 cggtaacaca cctgaaaaag caaggtgctg tccaacagcg tgataccggc attattcggc
     7501 ggcgtataag accacgaaac cgtgatggtg ccttcgggca ggttgggcgg aagccccatg
     7561 ttggcttgtg agaagttggg caggttgccg ggaataacta cgttttggaa actcaacact
     7621 gccgggtcgt aattaattgt aaactgcatg ctgttgagat cgtcgaagcc ggtaagacgg
     7681 tagtccacgc aaatcaaatc gcccgaattg ccctgttcct gcgaagcaat cagttctacc
     7741 ggacgcacac aattggttac aattccgtca ttgttgataa gaccgacatt gaggcaggag
     7801 ctgttgtctt tggtcacata aggattgggg cctacgccaa tgtttgtgct ttctccgtaa
     7861 gtatcggaaa gcatggtaaa gcacaacttg aatatcgaag gtcggcattg cccgaagcag
     7921 tcgtctaatg taacgcccgt tgcacccgga gtgttgcagt tggccacgct ccaactgatc
     7981 gtcagaacgc cgctcgcagc agcactggca tctacgttgg tacccaccgt aaagcccggc
     8041 aaagtaggca tggtgaggtt gacgtattgg agtatcgtcg ggtcccagcg aatttggtag
     8101 ctggttcctg tgatattgga aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctcaatgtt
     8221 atccataaaa ttatcttttt catgcgcaga ttggatttgg acaaaaaaag ttgtcaagtg
     8281 tcatgggaaa gcggatcagc gatccaaaga gagcgtcagg ttgatttcat gtgtagcgcc
     8341 tatgctcggc ccgaaggaac tgaacgaata gtcaaaacca tacccgatgc ggaatatatt
     8401 gtcaaagccg aggttctgcc ccagcaggaa gccggtttcc aaatgtacgg ccttggccga
     8461 agacgcgccg gcgcccaccc agaggctccc cggaatctgg tagcgcagat tcacgtccac
     8521 gttgatcggg gcgttgggcg catacttaaa caggacggag ggttcgatga aaccatcgtc
     8581 gcccacaaac ttgtaaaagc ctatggagcc gtagatgtgc tgcacgcgtt tggtgaaaaa
     8641 ctggccgttt tcgtttttaa acgtgaggtc caatcccaat gcctgcggca tagacaagcc
     8701 ggtatagaag aagttgccgt ctcccaccat ttggtaataa tacagaccca ggcccacatc
     8761 agggaagatg cgcgtctggt cggcgctgcc caatacgtcg ccgtcgtcgc gcagtaccaa
     8821 ctcggaggct ttcacccgat attgtaccgc gccggcagta agagccagag ccagaccgcc
     8881 atactcggcg tcgccggtaa tgacgccgcc gattcgaccg taaatgccgg tgaatccggt
     8941 aggcccggtc tggtcattga cgatgtgccc gccaaacatg ggcgtaacac cggcataacg
     9001 tttgttgaca aacgacatgc gcaaagtctg cgtacggggc gcagatttga tgccggtcca
     9061 ctgcgctctg taagaggcgc cgaaggtcat gttgttgcca aaggtgagaa aatcgctttc
     9121 catagcggcc ggattaataa tcacggcgtt ttcacggtac tgcgtgaaaa gagaaagctg
     9181 ttggccatgc gatgcaatac tacatagcag taacgccaca ggcagaagcg tgcgtaatac
     9241 gttcatggga cgagtgcgtt tttgagattc ccaaaatgat tgattcaaat attctgtgcc
     9301 acccacgaca ggaggctgtt gttaatgctt cgtgtgtcgg atattcgtgg gctttctcac
     9361 agtagctttt gaagagattg aaaagcggac gcaaatatcg ctgcctttct caaaaattct
     9421 atacctgagc gagaataatt tataaatcgg ctgtttttta actttttttc agtcggcagg
     9481 cttacgaagc gcgcgctatc caccggagcg cttggagccg gaatagcccg cctgcaataa
     9541 aatttgcaac acccgctcgc ggtgatcgcc ctgcaccatg atctcgccgt ctttggccgc
     9601 gccgcccacg ccgcacttgg ttttgagcat tttgcctaag gcttccaatt cggattgcgg
     9661 cccgacgaat ccggttacca aagtgacttc tttgccgccg cgctgcttgc gatccaaaga
     9721 tacgcgcagc ttttgctgtt gggcgggcaa caactcggct tcctcctgct cttcgtagcg
     9781 ataggcaaaa tcagggtcgg tggagaagac gacgccgtcg cggtttttgt tctttttact
     9841 cattttttgc tcggttggac tgcgggcaaa ggtacatatt cctgcacaag cagaaagcaa
     9901 cacagaattg tagtgtgatt gtgattattg ttgagcctac ttattcgaat tgttttgaaa
     9961 atatgttctc aacttttctc tcagttctgc ctctacttcc aattttccgg ccagattcac
    10021 ggtttcctga ggttcggaaa ggtaatcata cagttctacg gccagcaggc gtccatccaa
    10081 cggagattgg ccttccgtat atttgcaaga gtaccaggcc gtatatcggt agcgctgcgt
    10141 acgcaaagaa taacccattt cattcagata aggaaacatg ccctgccaga aatttgcctt
    10201 ggcttggccg gcttccacag gctcatttcc ccatggataa accatattgc tcaatccgcc
    10261 acgagcggcc cattcccatt cggcctctgt gggcaaacgc ttgcccgccc acttgcaata
    10321 agccgtcgca tcttcccagg ccacttgtac caccggatgg ttcatgcggt cttcaatgct
    10381 gctgccaggg ccttccggtt gtttccaatg tgcgcccaaa gtccaggccc accagcgtcc
    10441 gggatcgtct aaaggcacgg gatgcttgga ggcccgaaat accaatgcgc ccggctgcag
    10501 gaggctgtct gccggcttgg gagtgccggg aggcaattct ttttgcattt cctcccaaac
    10561 aatggggcgt tctgccacgg tcacataacc ggtagctttc acaaactcag caaactgagc
    10621 attggtaact tcatgctcgt ccatccaaaa agcatggatt tctacatcgt gtttgggaaa
    10681 ctcgttggcg tcggcctgct cattgtcgcc gcccatttgc aacatgccgg acggaacgag
    10741 taccatgccg tcttttcggg acacggcttt gtcggccgat gaaaaaccgg cctcgttcat
    10801 acatgcgctc acaacgactg ctaagcatat acaacatgct gcaatgctct cggtatggat
    10861 gattttcatg tcgctttttg agaggcaagt taattacacc aatgcaggaa atggttgttg
    10921 aaaaaccgga taagttgatt tcccaaccat tcatgacaat ttttcaacct tttttcttca
    10981 acattaccca gccttccggg cctgtacgca tcatttcaac accacggtac ccaattccag
    11041 cgccagctcg tttctgattt ccagcaggcg ttggtgtact ttgaggtggg ccgctaagcg
    11101 ctccatgtct ccggcagcgg tgagttcttt gatgcgggtg tgattgcgct ccgacatgcg
    11161 catgattttg cgcaactgaa ccgtttcagc gcttgcaggc tgtcttcctg aaaattgtcg
    11221 tcgggcatct tttgcatgcg caggtatatc tcccagcgat tggcccagtt ttcactgtat
    11281 tcgtaaggct cgctcaacag gtttacggcc agcagactga tctccgggtc ggaatggtgc
    11341 aggaaataat tggtgttcag ttgcgagcgg cggtttgcca gacaggccag cacctcgcgg
    11401 gccactttgg cgtacaaggg gttgtcaaac tcgtccaata catcctctat attgctgata
    11461 atgtactcag ccacagatag atggtcgtct ttgtcaaact cctcgtgact gaaagcaatg
    11521 agtatgcggg ctatgtcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    11581 gccttcctcg ctgcctcctg ctccggcgct tgccccgcag gtacgggtac ggcatctgta
    11641 tcgctgctga cgtcggcagg cgagcgctct ccttctctgc ccggtgcgtg tcttttttgc
    11701 atttgggcaa gtaccaattt gttggtctcg ctcatcagaa tggcctcgtc cacctgcgcc
    11761 acgcgggcac actcctgtac gaacagcgag cgcttcaccg gatcgggtat tttggcaatg
    11821 ctcgtcacaa tatcgcgcac cacgccgctg cgtttcaccg ggtcggagcc ggcctcctgc
    11881 atcagcaagt ctgctttgaa cagaatgaag tctttggcct gccgggcaat gtattctgta
    11941 aatgccgtgg cgcccactct ggcgaggtag gaatcggggt cttcgcggtc gggcagcagc
    12001 acgatgcgca cattgaggtc ctgctccagc accaaatcca atccccgcag cgccgccttg
    12061 atacccgccg ggtcgccgtc gtacaggatt ttaatgttgt tggtattgcg tttgatgagg
    12121 ccgatctgct ctaccgtgag ggcggtgccg gaagaggcca ccacattttc gatgccggcc
    12181 tgatgcagcg aaatgacgtc ggtataaccc tccacgagga tgcactcgtc tttttcgcgg
    12241 atggctttcc gggcctggta ggcgccgtac aacaccttgc ttttcaggta gatgtcggtt
    12301 ccggcgaatt gatgtatttg ggcgctttga cgtctttggc catgatgcgc ccggcaaagg
    12361 cgatgacctt gccgctcatg ccgtgaatgg gaaagatgac ccggtcgcga aagaaatcgc
    12421 ggggcggcag gttgcggctt gcatcggggg ggctgcacag gccgagattg aacaacatct
    12481 gctgatcgta gccggcggca gtggcggtgg tgaggaactg atcgcgcttt tcgggcgcat
    12541 agcccagtcc gaatttctga atggtttctt cccggaagcc gcgttcacgg aagtagctca
    12601 aacccacgct gcggcctcgg tcggtattga caagctgatt ttgaaagaat tccagcgcat
    12661 actgattgat gatgtacagg ctgtcggcta cccggcgctc ctcggcagcc tcgcgggaac
    12721 tctcaatttc ctctatctgg atgccgtatt ttttggcaat gtatttgagc gcctccggga
    12781 aggtgaagcg ctccaactcc atcaaaaact gcacggcatt gccgcctttg ccacagccga
    12841 aacacttgaa aatgttgcgc gccggcgaga cgttgaagga gggcgttttt tcgtgatgaa
    12901 aagggcacag gccgatcagg ttcgtacccc tgcgccgcag cgaaacgaag tcttgcacca
    12961 catcctcgat tttgacggta tcgaggattt cctgaacgct tttttgagtt atcatcggat
    13021 caaaaagaac tcaacggatt tcggcgagaa tcgaatattg tctgcatgat cgggccggcg
    13081 ggtcaattgc agcagtgcgg tgttgcgctt ggcaaagcag cgcccctcag atcggcagtt
    13141 atttcaaagt cctcagcaat cgtgctgtcg tatcggctca gccccagcgt gcaggtgatc
    13201 tgaatgtttt tggggaaaac ccgaacactg tcggcatcgt gcaataccgt gacgggcacc
    13261 caaaatgttt tttccgacaa ttgctctacc gaaattttca ctttcacctt gtcggcgctg
    13321 atatataccc ccttgggagg cgggcggaga tcaaccgttt gatccaccgt ctttttcagt
    13381 tgttccagca ccaccgaatc ggtaggccac tcccctaccc cggccaattg agactccggg
    13441 ccggtcaagg tcacgctgga cacaacctcg cgcctcctca cccaccgcac
//
LOCUS       DAORLF010000061        13374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2475_length_13374_cov_2.297920, whole genome shotgun sequence.
ACCESSION   DAORLF010000061 DAORLF010000000
VERSION     DAORLF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_13374_cov_2.297920"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>354
                     /locus_tag="PK198_07265"
     CDS             <1..>354
                     /locus_tag="PK198_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38570.1"
                     /translation="TSGDYQLCLTAANACGTAPQTCRTITVPAVPTTQINANICFGDC
                     VTIADTTICQPGTFQRRLTAASGCDSIVRIVLTQEQPVVTNLHLFICEGDTFFVANRP
                     FTQSGVFQEVMTAAN"
     assembly_gap    355..384
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..1667
                     /locus_tag="PK198_07270"
     CDS             <385..1667
                     /locus_tag="PK198_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF38571.1"
                     /translation="ICEIRGSITPTSVRCRGSSDGRLEFSVLNGTPPFTYTWQRIGQG
                     APAGQGSILGLNQAQILPNLPPGQYVVTINDMFGNDVILLADITEPPALSLSLLASDY
                     NGFGIDCAGAATGTLTTSSGGGIPPYNLIWSNGASGQSISMLTAGSYGVTLTDANGCT
                     QTAEALLTQPAPLMLNAAFTNPDCEGPLTGSVLAGATSGGLAPYEYALQNAPFGPDTL
                     FAGLAEGAYQLSLRDANGCISSVSGTLTAAAIPQIELGEDRTLELGESLQLNAVVNIP
                     VTQMQWSPATALSCTDCYDPLATPLRSITYTFSGVSADGCIGVDSVQILVLPFRDVFV
                     PNAFSPNDDGVNDHLAVFGGKEVLKIRTFRVFSRWGELIFEGRDFEPNVPPAGWDGRF
                     KGRPMGQGLYAWVAEIEFIDGVVAVYEGEVTLLR"
     gene            1853..2533
                     /locus_tag="PK198_07275"
     CDS             1853..2533
                     /locus_tag="PK198_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF38572.1"
                     /translation="MRVLLIEDEIAISSFIAEGLQEEGFAVDVAHNGREGLILALENL
                     AEYDLVLLDWMLPGVSGIEICRAIRKENTTLPVIFLTAKDTVDDVVFGLETGANDYLR
                     KPFAFEELLARMRVLLRGSTGEQNVFNCRDIELNVATHRVLKAGRPVELTQKEFALLE
                     LLLRNKGKACRRTRIIEKVWDINFDHDTSVIDVYVNFLRKKLDTPGQASFIQTLRGIG
                     YRINDDET"
     gene            2530..3906
                     /locus_tag="PK198_07280"
     CDS             2530..3906
                     /locus_tag="PK198_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF38573.1"
                     /translation="MTLSFKNRIALFTALAAALTIGFVFLMVYGVVYFSAYNHLDEDI
                     YQEKEEVLNNLRLKGDSIIIESLPEWDEKEHQQTEVNPVFLQVVDKNGKLLFRSSNLR
                     ESVLLFNPATDQDAFFNSHIENQRVRQGQFPILNEQNFLIGHLSVGLSSQESSIVLHN
                     LRNTLWAAFPALLLVLFWATSFAASKGITPVQQLIKAASVIDENHISNKLPLPETKDE
                     IHQLAAAFNELLHRIDNSMQREKQFTADASHEIRTPLTAIRGTLEVLIRKQREPAQYQ
                     EKISQVIREVDRLHAMLDQLLQLARLEGGRTSVHEIPLQINALLASAADKWQPRLAEK
                     NMALHFDIPPSEVVTTDPDLLGVVVDNIIGNAIKYGRNGGRIDCLWETGSRSLIIRDD
                     GPGIPPEQLPYVFDRFYRTDDSRSSSVPGTGLGLAIAKKMSDLLNIRLTANNAKEAGA
                     EFILRFPF"
     gene            3988..4779
                     /locus_tag="PK198_07285"
     CDS             3988..4779
                     /locus_tag="PK198_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRF38574.1"
                     /translation="MKKNILIVVLMAVAGILMMPSCNSNADKNNSLQAPETTQSETNQ
                     ATAAANGEQDDDDDDDALENQGQAPAKTNPAGSTDAKTAIINNLMTAFTGETTASAKY
                     AAYAKKAQEEGFVQIALLFKATSRSEMIHADNHRAVLEDMEKTAPSVSPQFSVKTTRE
                     NLADAIAGEAYEISTMYPDFMATANTAVNQQALTSLNYAYRTEQRHKPLYEKALAALQ
                     NNQTGTLPGQYLVCPTCGNTYDAAPPKRCRVSMTPSERFIKINSL"
     gene            4806..5303
                     /locus_tag="PK198_07290"
     CDS             4806..5303
                     /locus_tag="PK198_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38575.1"
                     /translation="MNQAHLHLLFNHLPILGTLFGLLILVSGYFVKSNAVKRTALGIF
                     MLSALCTIPAFLTGEGAEEMVESLPGVTENLIETHADMADIFLWVVGALGLFSLATFY
                     ADLKAQKIAPVLYGITLAVAIGTMALAQQVGATGGKIRHTEIRNGVATAGAGNMENNN
                     EEDDD"
     assembly_gap    5390..5402
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5440..5850)
                     /locus_tag="PK198_07295"
     CDS             complement(5440..5850)
                     /locus_tag="PK198_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38576.1"
                     /translation="MKAGYRYVPYSSMEAIIEQEKQSYYLSLRQTQTTLNLAQTNWQS
                     WLLFFLKSLKRQKDNLEIKVSREHILLTQMPELAQNILEIVRSRGRINMGELELLTNA
                     NRSTLRKSLENLVKNKQLQQNGVGKGTWYSLFTS"
     gene            complement(6057..7325)
                     /locus_tag="PK198_07300"
     CDS             complement(6057..7325)
                     /locus_tag="PK198_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRF38577.1"
                     /translation="MKEDFLHYLWRAKRFDLADLRTTQGEPVEILQWGEYNRHAGPDF
                     QQTRLRIGETLWAGSVEMHLRASEWLRHRHQDDDAYRNVVLHVVLEEDVPVHRPDGDR
                     LPCLELRHRIAPRIQGAYLQLLHNQHWIPCQKQFHTVPEITRSLWLDRLAVERIQQRT
                     GAVQLVLEQTNNNWEETFYRLLARSFGVRVNTDPFEQLARSTPLLLLSKYKNNLFRLE
                     ALLFGQAGLLQQEFRDEYPKKLQREYRFLQQKHELRPMSPAQWKFLRMRPASFPSIRI
                     AQLAALIYQSEHLFSKVLAATNVAEVENMFAVKISHYWQDHYVFDKASAPKAKSLGRD
                     AVHLLLINTIVPILFYFGKQRGEIRYQDKALELLDALEPEANSIIENWRQLGVQPTSA
                     CETQALLQLKQQYCDQHRCLECAVGAAILK"
     gene            complement(7408..7500)
                     /locus_tag="PK198_07305"
                     /pseudo
     CDS             complement(7408..7500)
                     /locus_tag="PK198_07305"
                     /inference="COORDINATES: protein motif:HMM:NF020444.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="element excision factor XisI family protein"
     gene            7614..9716
                     /locus_tag="PK198_07310"
     CDS             7614..9716
                     /locus_tag="PK198_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38578.1"
                     /translation="MSNLTFQYPAWYLLLCVLLGAGYALTLYFRDKTFQEQASWFTVL
                     LGVLRAVAVTAIAALLLSPLLRSLLQDVKKPVILLAQDASESVGLALSGDEARQQYKT
                     AFEQLTAELGKDYEVKSYSIGETAREGIDFEYKDKITNISQFLTGAYDLYSNQNLGAI
                     VLATDGLYNEGSNPAYLSNQLSVPIYSIALGDTSSRRDVSVKRAFHNKIAYLGDKFTV
                     QIDLAARNAAGASSNLSIFKVEGGGSRKLQEIPISIDRNDFFTTKEIVLDADRAGVQR
                     FRITAGAVSGESVSANNSKDIFIEVLDARQKILVLAASPHPDLSAIKQSLSSNKNYEV
                     TVSVISDFKQNVADFDFVILHQLPSRTNDATGILNTLQTRRIPRFFIAGMQTNFARLN
                     QVQSLVNIKTDGRNTNEVQGRISPEFSLFTTDERAARELPAFAPVIAPFGEFSVSPEA
                     QTLLYQRIGKVDTKYPLLVLGEQSGVRTAVFCGEGLWKWRLFDFLQNENHQLFDALLG
                     KAVQYVGIKEDKRKFRVNQPKTIFNENEPVVFDAELYNDNYELVNEPDAALVITNNKG
                     EEFKYTFNKSSRAYNLNPGIFPVGDYSFLASVSFSGQTLKYDGRFSVEPIQLELYETT
                     ADHGLLRLLSDKFGGEVVYPGDMASIAEKIRAKESVKPVIYNTTQTRPVIHLKWLFFL
                     LLGLLSLEWFLRRYFGGY"
     gene            9809..>10677
                     /locus_tag="PK198_07315"
     CDS             9809..>10677
                     /locus_tag="PK198_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38579.1"
                     /translation="MEIANPIYDVVFKYLMEDNKVAKLFLSAVTGLDIRSLEFLPQEL
                     VSNKKKSQKKTFLTALNLSIYRLDFSTKVREADGSEKVIIIEIQKSKFTHENMRFRKY
                     LGKQYMNETFFQWIVETTGRRYKSGLPILPIYILGEKVEGYDDMPVINVNRCIRDRYT
                     REVVEVRNHFIESLFHEGIIINVPALSQKRRDELEMLLSVFDQKNRSENHHIMNVKEA
                     DFPERFRPIIRRLQAAAQEKEVRDIMTVEDDFVAELNDYEHRIEEADKQKEEERRLKE
                     EAVRKQEEAVRMLL"
     assembly_gap    10678..10687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10861..>12261)
                     /locus_tag="PK198_07320"
     CDS             complement(10861..>12261)
                     /locus_tag="PK198_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF38580.1"
                     /translation="AQSLVINVISAANAALPACPVNLPAPVQVQVEALREAGTPIMGA
                     PVCAFTDTLVNLNQLLMGADWGGQWSLFPGSAQPFAGSFQAGAGSFRVTPNDAGSFRF
                     RYIVAAGAACPADTAVASLELLPLPPANAGPDQTLDCLHRVVSIGRAGNAAFRYLWTS
                     ADGRLIDDDMPIVEADAPGLYRLEVLNPLTGCRAFDEMRVISEIAVLEPQTGVGQISC
                     YGADDGWIQVQAVGQATPPLQFSLNGAPPVPQGLFPRLSPGLYELLIRDAAGCETTLR
                     FDLQQPSELQAVIDAPGLAGDPPSIQLGDSLRLELLTNVPPAEVASIVWSPAPCPECM
                     SVSLRPSFTDTYAVTVTNQNGCSASAELTVLVDRRPAVFIPNAFSPNNDGFNDRLLVF
                     AGKQVSHVRSFLIFDRWGEAVYEVYQFPPNDPAYGWDGSYRGQPLQSAVFVYVAEVET
                     IDGEVHLLKGDVTLVR"
     assembly_gap    12262..12293
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12294..13362)
                     /locus_tag="PK198_07325"
     CDS             complement(<12294..13362)
                     /locus_tag="PK198_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38581.1"
                     /translation="MLNLSGNGGAMQGDTAFVVTGLMPGQTVNIELTAHSANACPDAT
                     AAATCTTLPCPDVQIDIAPVPAVCWDGQADTIALSAQLSGAPATGVLRWEGEGVADTA
                     LGTWVSAANMAGRSITVYAVFTDDVCVFRDSASIAVWPVPTSDISADSVVCVADMATV
                     QYLGNAGAGAVFNWNFGGATAVPGNGAGPQSLSFASAGNYMITLSVEENGCAGAPDTA
                     LIQVDALLQMPVVQCETTYTSVVFSWPAIVGATAYQVILPAGIIPVWTSDTSIIISNL
                     APGTTLPISVEAVSETACPNTSAQSSCTTLPCPPVSLTMTATPVVCIGDSAQVQFSLA
                     GPAGTLLDLVLSDGVQTWPLAG"
BASE COUNT         3253 a         3613 c         3476 g         2947 t
ORIGIN      
        1 ccacgtcagg agattaccag ctctgcctca ccgccgccaa tgcgtgcggc actgctccac
       61 aaacttgccg aaccatcacg gtgccggccg tgccgaccac ccagatcaac gccaatatct
      121 gcttcggcga ctgcgtcacc atcgccgata ccacgatatg ccaacccggc actttccaac
      181 gccggctcac ggcagcctcc ggctgcgaca gcattgtgcg catcgtcctc acgcaggagc
      241 agcccgtcgt taccaacctc catctcttca tctgcgaggg ggatacattt tttgtcgcca
      301 accggccctt cacacaaagc ggtgtttttc aggaagtaat gaccgccgcc aatannnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnttattt gcgaaatacg gggcagtatc acgcctactt
      421 cggtgcggtg ccgaggcagt tccgacgggc gattagaatt cagcgtcctc aacggaactc
      481 cgccatttac ttacacctgg cagcgcatcg ggcagggcgc accggcaggg caaggcagta
      541 ttttagggct gaatcaggcg caaatcctgc ccaatctgcc accgggacaa tacgtcgtca
      601 ccatcaatga tatgttcggc aacgacgtca tcctcctggc cgatatcacc gaaccgccgg
      661 cgctgagcct ctccctgttg gcaagcgatt acaacggctt cggcatagac tgcgccgggg
      721 ccgccaccgg aacgctgaca acctcctccg gcggcggcat acctccatac aacttaattt
      781 ggagcaacgg cgcatcgggc caatccatct ccatgctcac tgcgggcagc tacggcgtca
      841 cgctcaccga tgccaacggc tgcacacaaa cagcggaagc gctgctgacc caacccgcgc
      901 ccctgatgct gaacgctgca tttaccaacc ccgattgcga aggcccgctc accggttccg
      961 tcttggccgg ggcgacttcg ggaggcctag cgccttatga gtatgctttg caaaacgccc
     1021 catttggccc cgacaccctg ttcgcgggct tggccgaggg cgcttaccaa cttagcctcc
     1081 gcgacgccaa tggctgcatc tcctccgtat ccggcacact gacggcggct gccatcccgc
     1141 agattgaact gggcgaagac cgaacactgg aattgggtga aagcctgcaa ctgaatgccg
     1201 ttgtcaatat accggtgacg caaatgcaat ggtcgccggc cacggcattg tcctgcaccg
     1261 attgctacga ccccttggcc acgcctttgc gcagcatcac ctatacgttc agcggcgttt
     1321 cagcagacgg ctgtatcggc gttgactcag tgcagatatt ggtgctccct ttccgagatg
     1381 tatttgtacc caatgcgttc agtcccaacg acgacggggt gaacgaccat ctggctgtct
     1441 tcggtggtaa ggaagtactg aaaatcagaa ccttccgcgt gttctcgcgc tggggcgaac
     1501 tcattttcga gggccgcgat tttgagccga atgtcccgcc cgccggctgg gacggcaggt
     1561 tcaaaggccg cccgatgggc cagggtttgt atgcctgggt ggcggagatt gagttcatag
     1621 acggcgtcgt ggcagtgtac gagggcgagg tgacgttgtt gaggtgagat tggagtaccg
     1681 aattggtcaa attatgtcaa acatcggctt taggaacggg gcctattgca agatagtcct
     1741 gccgcggcag atattttaat ccatttttaa ggaaaattca agcatcattt aaggtcgtac
     1801 catgcagatt tgcaaaacat tatggaatat tacgtaacaa aacagcagcg gcatgcgagt
     1861 tttactgatt gaggatgaga ttgcaatttc cagttttatt gcagagggct tacaggaaga
     1921 aggttttgcc gtcgatgtcg cgcacaacgg tcgggaaggc ttgattttgg ctttagaaaa
     1981 tctggctgaa tacgatctgg tactattgga ctggatgttg ccaggggtga gtggcatcga
     2041 aatatgccgg gctatacgca aggagaacac gacattgccg gtcatatttc tgacggcaaa
     2101 ggatactgtg gacgatgtcg tgttcggact tgaaacaggc gctaatgact atttgcgaaa
     2161 gccttttgcc tttgaggaat tgctggcacg aatgcgggta ttgcttcgag gaagtacggg
     2221 agagcaaaat gtattcaact gccgagacat cgaactcaat gtcgcaacac atcgggtgct
     2281 gaaggctggg cggcctgtgg aactgacaca aaaagagttt gcattgttgg agctactgct
     2341 tcggaacaag ggcaaggcct gccgccgtac tcgtattatc gaaaaagtgt gggacattaa
     2401 ctttgatcac gatacctctg tgattgatgt ttatgtcaat tttttgcgca aaaaattaga
     2461 cacacccggc caggcatctt ttattcaaac tttgcgcggc attggatacc gaatcaatga
     2521 cgacgagaca tgacattgag cttcaaaaac cgaattgcac tgttcacggc actggcggcg
     2581 gcccttacca tcggctttgt cttcctgatg gtgtatggcg tggtgtattt ttccgcttac
     2641 aaccatttgg atgaggatat ttatcaagaa aaagaggaag tactgaacaa cctgcgctta
     2701 aagggagatt ccatcattat tgagtcatta cccgaatggg acgaaaaaga gcatcagcaa
     2761 actgaggtca acccggtttt tttacaagta gtggacaaaa acggcaaatt gcttttcagg
     2821 tcttccaatt taagagaaag cgtattgttg tttaatcccg ctacagatca agatgccttc
     2881 tttaacagtc atattgaaaa tcaacgcgtc cggcaggggc aatttccgat tctcaatgag
     2941 caaaatttcc tcatcggtca tctttccgtt gggctttcat cgcaagaatc gtctattgtt
     3001 ttgcacaatc tacgcaacac gctgtgggcg gcattccctg cgctgttgtt ggtattgttt
     3061 tgggccacct cctttgctgc gtccaaagga atcacccccg tacaacaact cataaaagca
     3121 gcttctgtaa tagacgaaaa ccacataagc aataaactcc cgctaccgga aaccaaagac
     3181 gaaattcatc agttggctgc cgctttcaat gagttgcttc atcgcataga caacagtatg
     3241 caacgcgaaa aacaatttac ggctgatgcc tcgcatgaga tacgcacccc gctcaccgcc
     3301 atacgcggca ccttagaggt tcttatcaga aagcaacgcg aaccggctca ataccaagaa
     3361 aaaatcagcc aggtcatcag ggaggtggat cgcctgcacg ccatgcttga tcagttactg
     3421 caactggcgc gcttagaggg gggacgcacc tctgttcacg agataccgtt acaaatcaat
     3481 gctttgttgg cctccgcagc cgataaatgg caacccagac ttgcggaaaa aaacatggct
     3541 cttcattttg atattccccc ttcggaggtg gttaccaccg accctgacct actgggggta
     3601 gtcgtggaca atataatcgg caatgccatt aagtatggcc ggaatggcgg tcggattgac
     3661 tgtctttggg agaccggcag ccgttcgctc atcattcgcg atgacggccc cggaatcccc
     3721 cctgagcaat tgccttatgt attcgaccgc ttctatcgca ccgacgactc gcgcagttca
     3781 tccgtgccgg gaaccggatt aggtttggct attgccaaaa aaatgtcgga tttgctcaac
     3841 atccgcttaa cagcaaacaa cgcaaaagag gcgggcgctg aatttattct ccggtttccg
     3901 ttttaagcca attttgagaa tttctcaagg ttgtttaaag atgcccccct gcacctttgt
     3961 cttatcaaac caaaaaatga taagacaatg aaaaagaaca ttttgatcgt tgtactgatg
     4021 gccgttgccg gcattttgat gatgccctcc tgcaactcca atgccgacaa aaacaactca
     4081 ctgcaagcgc ccgaaactac ccagtcggaa acaaatcaag ctacggctgc cgccaatggg
     4141 gaacaagacg atgacgatga cgacgatgcc ctcgaaaacc aagggcaagc accggcaaag
     4201 accaatcccg caggttcaac ggatgccaaa acggcaatca tcaacaacct gatgacggcg
     4261 tttaccgggg agacaaccgc aagcgccaaa tacgctgcat acgccaaaaa agcgcaagaa
     4321 gaagggtttg tgcaaatagc cctgctgttc aaggccacct cccgatcgga aatgatccat
     4381 gccgacaacc accgtgctgt actggaagac atggaaaaaa cggctccgtc cgtctcaccg
     4441 cagttttctg taaagacgac gcgggagaac ctcgcagatg ccatagccgg tgaggcttat
     4501 gaaatctcaa cgatgtaccc ggattttatg gcaacggcca atactgccgt caatcaacag
     4561 gcgctcacca gcctgaatta tgcttaccgc acagaacaaa gacacaagcc gctgtatgaa
     4621 aaggcattgg ccgcgctgca aaacaatcag accggcacgc tgcccgggca atatctcgta
     4681 tgccctacct gcggcaatac ttatgatgct gcgcctccca agcgttgccg tgtctcaatg
     4741 acgcccagtg agcggtttat caaaatcaat tctctttaat ctgttcatac ttaaaaaaca
     4801 acaacatgaa tcaggcacat ttgcatctgc ttttcaacca cctgcctatt ttgggtacgc
     4861 ttttcgggct tcttatactt gtcagtggct attttgtgaa aagtaacgct gtcaaacgca
     4921 ctgctctggg catatttatg ctttctgcgt tatgtaccat acctgctttt ctcaccggcg
     4981 agggggcaga agagatggtc gagagccttc ccggagtaac agaaaaccta atagagacac
     5041 acgctgatat ggccgacatc tttctgtggg tggtgggcgc attagggttg ttctcattag
     5101 ccacatttta tgccgacctg aaagcgcaga aaatagcgcc tgttctctat ggaattacac
     5161 ttgcagtcgc aatcggcacc atggcattag cccagcaggt gggcgcaaca ggaggtaaaa
     5221 tccggcatac cgaaatcaga aacggcgttg cgacagcggg cgccggaaat atggagaaca
     5281 acaacgagga agacgacgat tagtggtcgg ttattgaata agccccgcaa gttacgttca
     5341 gtggggagtg gtggctacgc atgggagggg agaagttgtt gaggtgatgn nnnnnnnnnn
     5401 nntgggccgt cgccaggaga gtattcaaaa acgttgactt catgaagtaa acagggaata
     5461 ccaggttcct ttaccgacgc cgttttgttg caactgtttg tttttaacaa gattttcaag
     5521 gctttttcga agcgtgcttc gattcgcatt tgtcagcaat tccaattctc ccatgtttat
     5581 ccgccctctc gaacgaacga tttcaaggat attttgtgcc aattccggca tttgcgtcaa
     5641 caaaatatgc tctctactaa cttttatctc caagttgtct ttctgtcttt tcagtgattt
     5701 caggaaaaaa agtagccatg actgccaatt ggtttgagcg agattcagcg ttgtctgtgt
     5761 ttgtcgcaaa gacagatagt agctttgttt ttcttgctca ataatggctt ccatcgaact
     5821 gtacggcaca tagcgataac cggctttcaa caacagatat gtcgtcagtg ctctcgacag
     5881 gcggccgttg ccgtcttgaa aagggtgaat ggctaaaaag cacagaataa aagaatttca
     5941 ggtgtaattt ctaaagatcg tacagattgc atagcgcata tttttacgcc aaagtacgca
     6001 taaatgcact ttacttcata aaagtacgca taaaagcctg ccttccggca gatcagctac
     6061 ttcaaaatcg ctgcccctac ggcacactcc aagcaccggt gttggtcgca gtactgttgt
     6121 ttgagttgca gcaatgcctg ggtttcgcag gccgaggtcg gttgaacgcc caactgacgc
     6181 cagttttcga tgatgctgtt ggcttcgggt tcgagcgcat ccagcagttc cagcgcttta
     6241 tcctgatagc ggatttcgcc gcgttgtttg ccgaaataaa aaagaatggg cacgatggtg
     6301 ttgatgagca acagatgtac ggcatcgcgg cccagcgact tggccttggg cgccgaggct
     6361 ttgtcgaaca cataatgatc ttgccaatag tgcgagattt tgacggcaaa catgttttcc
     6421 acctcggcca cattggtcgc ggccagcact ttactaaaca ggtgttccga ctgatatatc
     6481 agcgcggcaa gctgtgcaat gcgaatactg gggaatgagg ccggacgcat gcgcaggaac
     6541 ttccactgcg ccggcgacat gggccgaagc tcgtgctttt gttgcagaaa ccggtactcg
     6601 cgctggagtt ttttggggta ctcgtcgcgg aactcctgtt gtagcaaacc cgcctgacca
     6661 aacagcaggg cttcgagccg aaagaggttg tttttgtatt tggagagcag cagcaacggc
     6721 gtggagcggg ccaattgctc gaaaggatcg gtattgaccc tgacgccgaa actgcgggcc
     6781 agcaggcggt agaaggtttc ctcccagttg ttgttggttt gttccagcac gagttgtacg
     6841 gcgccggtgc gctgctgaat gcgctctacg gcgaggcggt cgagccagag gcttcgcgtg
     6901 atttccggca cggtgtgaaa ctgtttttgg cagggaatcc agtgctgatt gtgaagcagt
     6961 tgcaggtagg cgccttgaat gcgcggcgca atgcggtgcc tgagttcgag gcagggcagg
     7021 cggtcgccgt cggggcgatg cacgggtacg tcttcttcga gaacgacgtg gaggacgaca
     7081 ttgcgataag catcatcgtc ctgatggcgg tggcggagcc attctgaggc gcggaggtgc
     7141 atctcgacgc tgccggccca gagggtttcg ccgatgcgga ggcgggtttg ttgaaaatcg
     7201 ggaccggcgt ggcgattgta ttctccccat tgcagtattt caacgggttc accctgggtg
     7261 gtgcgcaggt cggcgaggtc gaagcgtttg gcacgccaga ggtagtgcag aaaatcctcc
     7321 ttcatggttg gttggcgggt tgttgtatgg tatagtcgcc gaaggagggg aaattccaca
     7381 aacaggaagg gacttttttt tcaaaaacta cccgaccgca aagccggaat aggggcggac
     7441 aaaggcgggt tcaaaaccga gggcaatgtc ttcacgcgga actccacgtt ttacgagttc
     7501 atctgcgacg agccaaaaag tgaacgcaaa gctctacctt tgcgttgtag ttcggtttgc
     7561 gccgcccata caggaggcgc gccacagttc tgagtagttg aaaaaaaaag tagatgtcca
     7621 atctgacctt ccaatatccc gcctggtatc ttttgttgtg cgttttgctc ggagcgggct
     7681 atgcactcac gctgtatttc agagacaaaa cctttcagga acaagcctcc tggttcacgg
     7741 tgctgttggg cgttttgcgc gcggtagccg tcacagccat tgccgctcta ttgctgtctc
     7801 cgctgctgcg ctcgttgttg caggatgtga aaaaaccggt tattttgctg gcacaggacg
     7861 catcggaatc agtaggcttg gcgctgtccg gcgatgaagc ccgccaacag tataaaaccg
     7921 catttgagca actgactgcc gaattgggca aggactacga ggtaaaatct tacagcattg
     7981 gcgaaacggc ccgcgaaggc attgattttg aatacaaaga taagattacc aacatttcgc
     8041 agtttctcac cggcgcctac gatctgtatt ccaaccaaaa cctcggcgcc atcgtgctgg
     8101 ccaccgacgg cctctacaat gaaggttcca accccgccta ccttagcaat cagctctccg
     8161 tgcccattta cagcatcgca ttgggcgata ccagttctcg ccgcgacgtg tcggtcaaac
     8221 gggcttttca caacaaaata gcctacctcg gcgacaaatt cacagtacaa attgaccttg
     8281 ccgcccgcaa tgccgccggg gcttcttcca atctgtctat tttcaaggtg gaaggcggcg
     8341 gcagccggaa attgcaggaa attcccattt ccatagatcg caacgacttt tttactacca
     8401 aagaaatcgt gctcgacgcc gaccgtgcag gcgtgcagcg cttccgcatc acggcagggg
     8461 ccgtttccgg cgaatcggtg agcgccaaca acagcaaaga catcttcatt gaagtgctcg
     8521 acgcccgcca gaaaatcctc gtgctggctg cctcgccaca ccccgacctc tcagccatca
     8581 agcaatcgct gagcagcaat aaaaactacg aggtcacggt gtccgtcatc agcgatttca
     8641 agcagaatgt agccgatttt gatttcgtca ttctgcacca gttgccctcg cgcaccaacg
     8701 acgccaccgg catcctcaac accctgcaaa cgcggcgcat tccacgtttc ttcatcgccg
     8761 gtatgcaaac caattttgcg cgcctgaacc aagtgcaatc tttggtcaac atcaaaaccg
     8821 acggcagaaa taccaacgaa gtgcagggcc gtatctcgcc ggagttcagc ctgtttacca
     8881 ccgacgaacg cgcagcccgc gagttgccgg ccttcgcccc ggtgatagcg cctttcggcg
     8941 aatttagcgt ctcacccgaa gcccaaacac ttctttatca gcgcattgga aaagtggaca
     9001 ccaaatatcc gctgctggta ctgggcgagc agagcggcgt gcgcacggct gttttctgcg
     9061 gtgaaggttt gtggaaatgg aggctttttg actttttgca aaatgaaaat caccagcttt
     9121 tcgacgcctt gctcggtaag gcagtgcaat acgtcggcat caaggaagac aagcgcaagt
     9181 tccgcgtgaa ccagcccaaa accatcttca acgaaaacga acccgttgtt tttgatgccg
     9241 agttgtacaa cgacaactac gaactcgtga acgaacccga cgcggcattg gtcatcacca
     9301 acaacaaagg agaggagttt aaatatacgt tcaacaaatc gagcagggcg tacaacctca
     9361 atcccggcat cttcccggtg ggcgattaca gctttttggc atcggtgagc ttttcgggcc
     9421 agacgctcaa atacgacggg cgtttcagcg tagaacccat ccagttggaa ttgtacgaaa
     9481 ccaccgccga ccacgggctg ctgcgcctgc tcagcgataa gttcggcggc gaggtggtct
     9541 atccgggtga catggcctcc atcgcagaga aaatccgggc caaagaatcc gtcaaacctg
     9601 tcatttataa taccactcaa acccggccgg tcattcatct caaatggctg tttttcctgc
     9661 tcttgggcct gctctcgctg gagtggttcc ttcggaggta ttttgggggg tattaaatat
     9721 ctgcacatat tctaaaagtc aggctatcat tcgcgttttc gcaattgtat caaaaacccg
     9781 tatctttgca caaaatccgg ttctaattgt ggaaatagcc aatcccatat acgatgtggt
     9841 gttcaagtac ctgatggagg acaacaaggt cgccaaactc ttcctttcgg ccgttaccgg
     9901 attggacatc aggtcattgg agtttttgcc gcaggaattg gtctccaaca aaaagaaatc
     9961 tcaaaagaaa accttcctga cggcgctcaa tctgtccatt tatcgcttag atttttctac
    10021 aaaagttcgg gaagctgacg gcagcgaaaa agtgatcatc atcgaaatcc agaagtcgaa
    10081 gtttacccac gaaaacatgc gcttccgcaa gtatctgggc aagcagtata tgaacgaaac
    10141 cttctttcag tggatcgttg aaactactgg cagaaggtac aaaagcggcc tgccgatact
    10201 ccccatttac atacttggcg aaaaggtgga gggctacgac gatatgcccg tcatcaacgt
    10261 caaccgctgc atccgcgacc gctacacccg cgaggtagtg gaggtgcgca accatttcat
    10321 agaatcctta tttcacgagg gtatcatcat caatgtgccg gcactgagcc agaagcgccg
    10381 cgatgagttg gagatgctac tcagtgtatt cgatcagaag aacaggtctg aaaatcatca
    10441 tattatgaac gtcaaagaag ccgattttcc ggagcgattc cggcccatca ttcgtcgttt
    10501 gcaggctgcg gcacaggaaa aagaggtgag agacattatg acggtggagg atgattttgt
    10561 tgccgaactc aatgattacg agcaccgtat tgaggaggct gataaacaaa aagaagaaga
    10621 gcgcagatta aaagaagaag ctgttcgcaa gcaggaagaa gctgtcagaa tgttgctnnn
    10681 nnnnnnncac actgggggcg ccggcagaag acattgccaa aaagttgggg ctgaccctgg
    10741 agcacgtctt atcattggca aagcccacgc agaactgata gctccactcg gcctccgtca
    10801 tctgtccggt atgcggtgct gtttgcaact gcctgccaca tacgcatacc cacggcccgg
    10861 ctaccgcacc aaggtcacat cgcctttcag caaatgtact tccccgtcaa tcgtctctac
    10921 ctcagccaca tacacaaaaa cagcagattg cagaggctgc ccccggtagc tgccgtccca
    10981 gccatacgca ggatcattgg gtggaaactg atacacttca tacacggcct cgccccagcg
    11041 atcaaaaatc agaaaagaac gcacatggct cacctgcttc ccggcaaaaa ccaacaggcg
    11101 gtcgttgaag ccgtcgttgt tgggcgaaaa cgcattgggg ataaacaccg ccggcctgcg
    11161 gtccaccagc accgtcagtt ctgccgatgc gctgcaaccg ttttgattcg ttaccgttac
    11221 agcataagta tctgtaaatg aaggccgcag cgataccgac atacactccg gacatggcgc
    11281 cggagaccac acaatagagg caacttctgc cggaggcacg ttggtcagca attccaatcg
    11341 caacgaatcg cccaattgga tagacggcgg atcgcccgcc aagcccggcg catcaatgac
    11401 tgcctgaagt tctgatggtt gttgcaaatc aaatctcaaa gtcgtttcac agcctgccgc
    11461 atcccgaatc aacaactcat acaagcccgg cgacaggcgc gggaacaaac cctgcggcac
    11521 aggcggcgcg ccattgagtg aaaactgcaa gggtggcgtg gcctgcccga ctgcttgcac
    11581 ctgaatccag ccgtcgtcgg cgccgtagca actgatttgg cctacccccg tttgaggttc
    11641 caaaacggca atttcggaga tgacgcgcat ctcgtcgaag gcccggcagc cggtgagcgg
    11701 attcagcact tccaagcggt acaagcccgg cgcatcggcc tctacaatgg gcatatcgtc
    11761 atctatgagt cgtccgtcgg cactcgtcca caggtatcgg aaagcggcgt tcccagcccg
    11821 gccaatgctg accacccgat gcaggcagtc caacgtttgg tcaggcccgg cattggcagg
    11881 cggcagcggc aacaattcga gcgatgccac agccgtatca gccggacagg cggctccggc
    11941 ggccactatg taccgaaaac ggaagctgcc cgcatcgttt ggggtaaccc ggaagctgcc
    12001 cgcgcccgcc tgaaacgacc cggcaaacgg ctgcgcactg cccggaaaca aagaccactg
    12061 ccctccccaa tcggcgccca tgagcaattg attcaggttg accaaggtgt cggtgaaggc
    12121 acaaacagga gcgcccatga tgggtgtacc ggcctcgcgc aaggcttcta cctgcacttg
    12181 taccggcgcg ggcaggttca ccggacacgc aggcagggcg gcgttcgcag ctgatataac
    12241 attgataacc agggattgag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccccggc
    12301 gagcggccag gtttgtaccc cgtcgctcaa cacgaggtcc aacagcgttc ctgccggccc
    12361 ggccagcgaa aactgcacct gggccgaatc accgatacaa accaccggcg tggccgtcat
    12421 ggtcagcgat accggcggac atggaagcgt ggtacagctc gactgcgccg atgtgttcgg
    12481 gcaggcggtt tcgctcacgg cctccaccga tatggggagc gtggttccgg gggctaagtt
    12541 gctgataatg atactcgtgt cggaagtcca aacgggtatg atgcctgccg gtagaatcac
    12601 ctgataggcc gtggcgccga caatggcagg ccaactaaaa acaacgctgg tataagtcgt
    12661 ttcgcattgt accacaggca tttgcaacaa agcatccacc tgaatcagcg cggtatcggg
    12721 cgctccggcg caaccgtttt cctccacgct cagggttatc atataattgc ccgccgaggc
    12781 aaaactcagg ctctgcggac cagccccgtt gccgggaacg gccgtcgcgc cgccgaaatt
    12841 ccagttaaaa accgcgcccg ctccggcatt gccgaggtac tgcaccgttg ccatgtcggc
    12901 cacacacacc accgaatccg cgctgatgtc ggaagttgga accggccaca cggcgatgct
    12961 cgcgctgtcg cggaatacgc acacgtcgtc cgtaaaaaca gcgtaaacgg tgatgctccg
    13021 acccgccatg ttcgccgccg aaacccaagt tcccaacgcc gtatctgcca cgccctcgcc
    13081 ctcccaacgc aggacgcccg tagccggcgc gccgctgagt tgtgccgaaa gcgcgatcgt
    13141 atcggcctgc ccgtcccaac acacagcagg cacaggggca atgtctattt gcacatcggg
    13201 gcagggcagg gttgtgcagg tcgccgctgc cgtcgcgtcg gggcaggcat tggcgctgtg
    13261 ggccgtcagt tctatgttca ccgtttggcc cggcatcagg cccgttacca caaaagccgt
    13321 atcgccctgc atggcgccgc cgttgccgct cagattcagc acgctgtgtt gggc
//
LOCUS       DAORLF010000062        13255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2501_length_13255_cov_2.080455, whole genome shotgun sequence.
ACCESSION   DAORLF010000062 DAORLF010000000
VERSION     DAORLF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_13255_cov_2.080455"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK198_07330"
     CDS             complement(<1..823)
                     /locus_tag="PK198_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38582.1"
                     /translation="MTHTILSTITVIFGLTACMHSNHPETAENPIAASGMISPVTSTQ
                     SDSIIYDDFIFPVDSIFTAKVLTVGTFHKDEVFKNASRFKWYGLFKSQNGYYLKQTKI
                     KATNVYDPVLDEDEDEKTGWEIQAINEDTCLILIEPLSFLTDRIVQDAKLPEHYIYPG
                     DTIVWSYSGINYTIFATGKKKKVQEDPDWFDVWNYKLYLTATINGQQRKSLLVAQPGF
                     DDQMIYLIFAGDIDGDGILDLIIDTSAHYNATIPTIYLSKPAQAGELVKPVGGHRS"
     gene            complement(933..3356)
                     /locus_tag="PK198_07335"
     CDS             complement(933..3356)
                     /locus_tag="PK198_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRF38583.1"
                     /translation="MQHPILTKIAAARKQIASSIHSFTAPFLKPWHQFSARWPRTARW
                     TKRLFLLGFSMLTLLWVVYFSISLTVPTAKELKSIETQVASDIYSSDSILLGRYFKQY
                     RTVVRYEDLPTHVGQALVATEDARFFQHSGVDYVAWARVLFRTILRGDKTGGGGSTIS
                     QQLAKNLLPRRDFKYFSLIINKLREVAIARRLERAYTKEGILGLYLNTVPFSQNVYGI
                     DVAARRFFSKSATQLRPEESALLIGTLKATSSYDPVKAPQRAIERRNVVLSQMQKSSY
                     ISQKRRDSLQALPLQLSYNPIMRNEGIAPYFREFVRQEVERLLKDKRKPNGEAYDLDT
                     DGLKVYTTLHTDMQRIAEEAVEEHMSATQKTFDQHWKGMTVPWSDVQTIQLGVRNSLR
                     YKQLKLQGKSDQEIDAIFNTKVKMTVFSWDGPQEVEMSPLDSVRYHLGMLQVGFMAIE
                     PYTGYIRAWVGGINHEFFQFDHVKSRRQAGSVFKPVVYMAALRDSVQPCEQIRNQLLV
                     YHEYAKGDWAIKDWRRDDPEPHFAPDGKDLDDWIPQNSDGIYGGSYSMEGALTNSVNT
                     VSVSLIMRLGVQKVMQMAKNLGITGTIPAEPSIALGSAEVSLLEMIQPFTVFASEGRH
                     VPPVFITRIENYKGEVLADFTRQQPRQAISPEEAAIMTKMLQSVAIYGTASRIRWKYG
                     HHDIPLAGKTGTSQNHADGWFIGYTPKIVAGVWVGGDSPLVRFRNFENGQGAASALPV
                     WAYFMTRLKKDPAFAQWQGGEFPALSPDLIRRTACTFRIPSPDEMLSDSLDLELPEDE
                     IIEMPDPGR"
     gene            complement(3449..>3659)
                     /locus_tag="PK198_07340"
     CDS             complement(3449..>3659)
                     /locus_tag="PK198_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRF38584.1"
                     /translation="VLDGENLFTTPLPSSGLLIVGNESKGIRPATQQLLSHRIAIPKA
                     PGGGAESLNAAVATGIITACWINRV"
     assembly_gap    3660..3674
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3675..4212)
                     /locus_tag="PK198_07345"
     CDS             complement(<3675..4212)
                     /locus_tag="PK198_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRF38585.1"
                     /translation="MSVSKNDIKFVQSLRLKKFRQKYNNFIAEGEKIAGEALLSSRCK
                     VVRVFALPEWLHENAAQLSPIQASAITEAGENDLAKLSGLSTPNKVLMVLEVPQAGEL
                     PLIHTALYLDDVQDPGNLGAILRIADWFAVPQVFCSPGSVDVYNPKVVQASMGAFLRV
                     DSQEIELETLLRRQPELPV"
     gene            4227..>4584
                     /locus_tag="PK198_07350"
     CDS             4227..>4584
                     /locus_tag="PK198_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38586.1"
                     /translation="MLRKFFHRWFFCTFAGALLLLSAGCNTTRYLKDDELLLVKNEIV
                     LTDAKDVKNKGKLSYELTTLYKQRPNTKFFFVPRQWFYYRSNAPEDTTGFKKWQRRRL
                     AEAPAILNDTLTRRTEE"
     assembly_gap    4585..4671
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4672..6716
                     /locus_tag="PK198_07355"
     CDS             <4672..6716
                     /locus_tag="PK198_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF38587.1"
                     /translation="GRHKAEVRYYVAPGEVVRIDTVHFSSADTAIHRELQAIHSETVF
                     QKGAPLEGRLYDLEKERIAKHLRSNGYAYFYSNSFAELDADTTESQKKTTLYLEVIPP
                     PGESSHQRYTLGNITIYSQFDATLPDSIYRDTVVNGFLFKLREPVFLVRPQTILNAVH
                     LQTGDIYSQDSYDRSLQHLSSLGVFRFVRIRLDADKDDASVLHVRIELTQNKKYELGF
                     DLDANYIDGYTLTGRGSLMGLAFSPTAQNRNLFKGAELNITSLNAGVEVNFSKLNTNA
                     FWNSVELGLQNELYFPKFVDYLYLWRGMNKLPFGKKKLVSDALYQVLQDKAASRLSAR
                     YNYVFNFGWYETSLLNASFGYDVPIDNNTRIVLDHVGIDYLRPVTQPAFDTILMANPF
                     LARSFGKQLFVSLFFREFNLSKQRRPNRYGESGFNNLNIEMAGAEIWAGNALYNAFAL
                     SQDTLRISGTDFSQYVRIEWDTRYFKEYTPNLGFAARFNVGLARAFGFSTDVPYVKQF
                     FVGGPNSIRGWAARGLGPGGYRDTLSEGLTNRLLFYQTGDLKLEFNAELRFHVVSRLK
                     GAIFLDAGNVWTLRPDADRCGSQFLLSRRMSENCPVNLPFNDPFYKQIAVGTGAGFRL
                     DFKYFIFRLDLGVPLRYPRPVRNLMDTSPSEADYWHDFSNWRLRDVNFNFGLGYPF"
     gene            6948..8486
                     /locus_tag="PK198_07360"
     CDS             6948..8486
                     /locus_tag="PK198_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRF38588.1"
                     /translation="MLRAGILLLFSLWAVLSVQAQHRHHGYCGNGEGGEWDAMLTERL
                     LRNKQALEDQPVQFRSTIYVPLRIHMVANSSGFGRVTHAQVLDQLCKLNEDFADMDVQ
                     FYIKGLNDNINNNGIYTTQYSAGLVMNNLRDATALNIWIVDVAAGSQPSPGDLGVTLG
                     YYSPTRDWVVIRRDEVNSSSVTISHEIGHFFSLKHTHNGWDSTPWSPTIGNPAPVNSP
                     GGVPTERQDGSNCNTAGDYICDTPPDYNGFGFNGCNYNIAQDPTGAFINPDEQLFMSY
                     FLNCVRNDYYFSPTQQNLMIADYNSTARNYLRTSAVTPNLTQITSAATPVSPINNDFT
                     PGYNIVNFQWAAVTGAAFYLLETDVSAAFNSNPTRVVVSGATSHTMTTLLANRTYYWR
                     VRPYNAYRTCAPFSATAQFRTNNMTVSADNIEELNQWSVFPNPLAEDGRIQVQLQAAQ
                     GFEGRFLLYDVAGRMVRSMGSHRISGGYTQLELDAGGLLPGVYTLTLEHENGRELRRI
                     TVAK"
     gene            8519..>9060
                     /locus_tag="PK198_07365"
     CDS             8519..>9060
                     /locus_tag="PK198_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38589.1"
                     /translation="MKKLILSAVFMIFGMAVLQAQTNTGPCGNGGGEWKEMIIERLLR
                     NKQLLAEDPVQFRDVIYVPVRFHLVANSSGTGRVVFGRVLEQLCKLNEDYADFDMQFY
                     IKGVNDNINNTAIYNAQYNAGLVMNQLKDPNALNIWIVDVAAPAAPSPGDEGVILGYY
                     NPGRDWVVVRRDQVGASSVT"
     assembly_gap    9061..9070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9071..10054
                     /locus_tag="PK198_07370"
     CDS             <9071..10054
                     /locus_tag="PK198_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38590.1"
                     /translation="EVGHFFSLDHTHNGWDSTPWSSSIGNPAPLFSPGGVPTERQDGS
                     NCENSGDFICDTPPDYNGFGFNGCNYNIAQDPAGVFINPDEQLFMSYFLNCTRNNYYF
                     SPTQINLMMTDYNHSSRNYLRTGAVTPNLTEITEVPTLTFPINNETTPGYNIVNFQWT
                     AVAGADFYLLEIDRVATFTLNPIRIIVAGANSRSVTTLEANRTYFWRVRPYSAYRTCA
                     LSTPVATFKTNSTVVATQEIEALNEWTVSPNPIISQGALQISLQAEKSFDGDFSLFNA
                     AGQLVRSLGRHRVQSGATYLEFSMEGLSSGVYMLALSHEEGREIRRVVLAK"
     gene            10255..11043
                     /locus_tag="PK198_07375"
     CDS             10255..11043
                     /locus_tag="PK198_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HRF38591.1"
                     /translation="MSYHPSELRRVNIIRYVTPLREGGSLPAIVEADDDFLYVAKFRG
                     AGQGTKALIADLIGAEMARVAGLQVPELVLADLDESFGRTEPDEEIQDLLKFSTGTNL
                     GVHYLSGAISYDPVVNKAEPLTASKIVWLDALLTNVDRTARNTNMLVWNRELWMIDFG
                     AGLYFHHSWDNWEQQSQSAFPHIKTHVLLSQAAQLDEADELLRQAITSEIIAAILAAI
                     PDDWLHGHGSPDEMRAVYHDFLTRRIAASSTFLNTAKDARAGII"
     gene            11021..11404
                     /locus_tag="PK198_07380"
     CDS             11021..11404
                     /locus_tag="PK198_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HRF38592.1"
                     /translation="MQERELFEYAVIRWVPRVEREEFLNVGVILFCKKRNFLHVLYEV
                     KEDRLKALFPECELEEVKCHLDAFARIAAADTSAGSIAQLDAAGRFRWLTAQRSTVIQ
                     ASKVHPGLCTDPMEMLERLFGQMVG"
     gene            complement(11496..>12313)
                     /locus_tag="PK198_07385"
     CDS             complement(11496..>12313)
                     /locus_tag="PK198_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRF38593.1"
                     /translation="VGELVLIYTGDTYRQKIQLRTWNERIAEFQEKGSEMEQIPECLL
                     KLSLTEDTLIYKMVKIQEKGEGYEAEFSYPQFYESTGKKSQLNKEIELMVFDKKSDSK
                     TYFSTNEKAYQTYFKKTPEDEKHSWTDWEEVILIINSPHIISAIHSDFEHKSGGAIGY
                     GNNVGFNYDFEKGKKISFEELFDSNKLDTLQAKLTNQYKQHFHITSLKENGINGEYIP
                     ISTNFYIAKEGIVFLYQRFEHNGIGGGGMRIEIPYCQLIDVLREESVIKKHLK"
     assembly_gap    12314..12440
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12463..13227)
                     /locus_tag="PK198_07390"
     CDS             complement(12463..13227)
                     /locus_tag="PK198_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRF38594.1"
                     /translation="MLHRNAPLSVEGRRRLVERCQTRPLSHVAAEMGISRACASKWMA
                     RYREHGELGLLDRPSVPHHQPTATPAETVTRIETLRRSRKWSAARIAHELGADGIVSG
                     ALLPDGNIDLARIRLMLEAAEGLPFTFHRAFDMCRDPLRAIDELAELGVQRILSSGQQ
                     PNAALGFEHLRRFAQYAAGRLSIMACGSLMPDNVGPLTEVPEIQEFHAAVRGVVRSEM
                     QYRGAINMGDESADEEFNWMETDVSLVAGLKRGIAG"
BASE COUNT         2978 a         3340 c         3417 g         3281 t
ORIGIN      
        1 cacttctgtg cccgcctacc ggttttacca attctccagc ttgggccggt ttagacaaat
       61 aaattgtcgg aatggttgca ttgtaatgag cggaggtgtc aatgattaag tctaaaattc
      121 catctccgtc tatgtcgccg gcaaaaatca aatagatcat ttggtcatca aaacctggtt
      181 gggccacgag aaggcttttg cgttgttgcc cgttaatcgt agcggtcaaa taaagtttgt
      241 aattccaaac atcaaaccaa tccgggtcct cctgaacttt cttttttttg cctgttgcaa
      301 atatcgtgta gttaatgcct gagtacgacc atacaattgt atcgcccgga taaatatagt
      361 gttcaggcag ctttgcatct tgaacgattc tgtctgtcaa aaaggaaagc ggttctatta
      421 gaataaggca agtatcctcg ttgattgctt gaatttccca tcctgttttt tcatcttcat
      481 cctcatcaag aaccgggtcg taaacgtttg tcgccttgat ttttgtttgt tttaaataat
      541 acccgttttg actcttgaat aatccatacc atttgaatcg gcttgcattt ttgaatactt
      601 cgtctttgtg aaaagtcccg actgtgagca ctttggctgt aaatatgctg tcaacaggaa
      661 agataaaatc gtcgtaaata atactgtcac tctgcgttga agttacaggt gaaatcatac
      721 cactggctgc tattggattt tcggcagttt cgggatgatt ggagtgcatg caagccgtga
      781 gtccgaaaat gacagtgatt gttgataata tggtatgcgt catttctttt tcttcttttt
      841 ctcaggttgt ttttcagtat gtatccaagt ccaggtattg tttgattgta cagggggact
      901 tatgctttct gaggtatcaa tgattagtag gattacctcc ccggatccgg catttcaata
      961 atttcatctt ccggtaattc cagatcgagc gaatcggaga gcatttcatc gggcgagggg
     1021 atgcggaagg tgcaggccgt gcggcgaatg agatcgggcg acaaagccgg aaattcaccg
     1081 ccctgccatt gggcgaaagc cgggtctttt ttcagtcggg tcataaagta ggcccacacc
     1141 ggcagtgccg aagccgcgcc ctggccgttt tcaaaattgc ggaaacgcac caaaggcgaa
     1201 tctccgccca cccacacgcc ggccacgatt ttgggcgtat agccgatgaa ccagccgtcg
     1261 gcatggtttt gcgaggtgcc ggttttgcct gccaggggga tgtcgtggtg cccgtatttc
     1321 cagcgaatgc gactggccgt gccgtaaatg gccaccgact ggagcatctt ggtcataatg
     1381 gcggcttctt cggggctgat ggcctgccgg ggttgctgcc gggtaaaatc ggccagcact
     1441 tcgcctttgt agttttcaat gcgggtgata aaaacaggcg gaacatgcct gccttccgac
     1501 gcaaacacgg tgaaaggctg tatcatttcg agcaaagaca cctctgccga acccagcgca
     1561 atagacggct cggcaggtat cgtgccggtg atgccgaggt ttttggccat ttgcatcact
     1621 ttttgaacgc caagccgcat gatgagcgaa acggaaacgg tgttgacgga gttggtcaac
     1681 gcgccctcca tagagtacga gccgccgtag atgccgtccg agttttgagg tatccagtcg
     1741 tcgaggtctt tgccgtcggg ggcgaaatgc ggttcggggt cgtcgcggcg ccagtctttg
     1801 atggcccagt cgcctttggc atattcgtgg taaacaagca attggttgcg gatttgctcg
     1861 cagggctgaa cgctgtctct gagggccgcc atatacacca ccggtttgaa gacagaacct
     1921 gcctgacgcc gggattttac atgatcgaac tgaaaaaact cgtgattgat gccgcccacc
     1981 catgcccgga tgtagcccgt atatggctcg atggccataa agcccacctg caacatgccg
     2041 aggtggtagc gcaccgaatc gagcgggctc atttccactt cctgggggcc gtcccaggaa
     2101 aaaacggtca tttttacttt ggtgttgaag atggcgtcaa tttcctgatc ggatttgcct
     2161 tgtagtttca gttgtttgta cctcaatgag ttgcgcacgc cgagttggat ggtttgcaca
     2221 tcgctccagg gtacggtcat gcctttccaa tgctggtcga atgttttttg cgtggccgac
     2281 atgtgctcct cgacagcctc ttcggctatg cgctgcatgt cggtgtgcag ggtggtataa
     2341 accttcagtc cgtcggtgtc aaggtcgtat gcttctccgt tgggtttgcg tttgtctttg
     2401 agcaggcgtt ctacctcctg gcgtacaaac tcgcggaaat acggcgctat gccctcgttg
     2461 cgcatgatgg gattgtaact caattgcagg ggcaaggcct gtaaggagtc tcttcttttt
     2521 tgagaaatgt agctgctttt ctgcatttgc gaaagcacca cattgcggcg ttctatggcg
     2581 cgttgaggcg cttttacagg atcgtaagaa gaggtagctt tgagggtgcc gatgagcagg
     2641 gcgctttctt cggggcgcag ttgtgtggcc gatttgctga aaaaacgccg ggcggccaca
     2701 tctatgccgt aaacattttg ggaaaaaggc acggtattga gatacaaccc cagaataccc
     2761 tctttggtgt aggcgcgctc taatcggcgg gcaatggcta cttctctcaa tttattgatt
     2821 atcaaagaaa agtatttgaa atcccttctg ggcaggagat ttttggccaa ttgctgactg
     2881 atggtactcc cgccgccgcc cgttttgtcg ccccgcaaaa tagtgcggaa aagcacgcgc
     2941 gcccaggcca catagtccac gccgctgtgt tgaaaaaagc gggcatcttc ggtggctacg
     3001 agggcttggc ctacatgtgt ggggaggtct tcgtagcgta caacggtgcg gtattgtttg
     3061 aaataacggc ccagcaggat gctgtcggaa gagtaaatat cagaagcgac ctgcgtctca
     3121 atgcttttga gttctttagc cgtcggtacg gtgagggaaa tggaaaaata gactacccaa
     3181 agcagcgtca acatggaaaa gcccagcagg aaaagccgtt tggtccaacg ggcggtgcgg
     3241 ggccacctgg cgctgaattg atgccagggc ttgaggaaag gtgctgtaaa tgagtgaatt
     3301 gaggaagcga tttgcttccg ggcggctgct attttcgtga gtatgggatg ctgcatttta
     3361 gatgcgttgc tgttgcggcg aaaggtagaa gttttttttg ctaccctgta caaaatgaat
     3421 aaggccgact gataaaaaaa gttcagactt atacccggtt gatccagcag gcggtgatga
     3481 tgccggtagc caccgctgca ttgagcgatt cggcgccgcc gccgggcgct ttgggtatgg
     3541 caatgcgatg gctgagaagc tgctgcgtgg ccgggcggat gcctttgctt tcgttgccca
     3601 cgatcaatag cccggaagag ggtaggggag ttgtgaaaag attttctccg tctaatacan
     3661 nnnnnnnnnn nnnnatacgg ggagttcggg ctgtcggcgc aacaaggttt ccaactcaat
     3721 ttcctgagaa tcaactctta aaaatgcccc catcgaagcc tgtacgactt tgggattgta
     3781 aacgtccacg ctgccgggcg agcaaaaaac ctgcggaacg gcaaaccagt cggcaatgcg
     3841 gagaatggcg ccgagattgc cggggtcttg cacgtcgtcc aaataaaggg cagtgtgaat
     3901 gagcggcaac tcgccggcct gcggaacttc gagcaccatg aggactttgt tgggcgtact
     3961 gaggccggaa agtttggcca agtcgttttc gcctgcttcc gtgatggcgg aggcttgtat
     4021 tggagacaat tgcgctgcat tttcatgcaa ccattccggc agtgcaaaaa cgcgaacgac
     4081 cttgcatctg gaagacaaca gggcctcgcc ggcaattttt tcgccctcgg caatgaaatt
     4141 gttatacttt tggcgaaact tcttcaagcg caaagactga acgaacttga tatcattttt
     4201 ggaaacagac attgttggat agttgtatgc ttcgtaagtt ttttcaccga tggtttttct
     4261 gcacgtttgc cggcgccctg ctgcttttgt cggcaggctg caataccacc cgttatttga
     4321 aagatgacga acttttgttg gtgaaaaacg agattgtcct caccgacgca aaggacgtga
     4381 aaaacaaggg aaaactgagc tatgaattga cgactttgta caaacagcgc cccaatacca
     4441 agttcttctt tgtgccgagg cagtggtttt attaccgcag caatgccccg gaggacacta
     4501 ccggctttaa aaaatggcag cgccggcgtc tggccgaagc tcccgccatt ctgaacgata
     4561 ccctgacccg ccgcaccgaa gagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaggacggc
     4681 acaaggcgga ggtgcggtat tatgtggcgc ccggcgaggt agttcggata gatacggtgc
     4741 atttcagttc tgccgatacg gccatccatc gggagttgca ggccatccat tccgaaacgg
     4801 tttttcaaaa aggtgctcca ttggaaggaa ggttgtacga ccttgaaaaa gaacgcattg
     4861 cgaagcatct tcgcagcaat ggctatgctt atttttattc caacagcttt gcggaattgg
     4921 acgccgacac taccgaatcc cagaaaaaaa ccaccctcta tctggaggtc attcctccgc
     4981 ccggcgagag cagccatcag cgctacacac ttggaaatat taccatttat tcgcagtttg
     5041 acgccaccct gcccgacagc atttaccggg acaccgtagt gaatggattt ttgttcaaat
     5101 tgcgcgaacc ggtgtttttg gtgcggcctc aaaccatcct caatgccgtg catctgcaaa
     5161 caggcgacat ttatagtcag gatagttacg accggagttt gcagcatttg tcttcgctgg
     5221 gagtgtttcg ttttgtgaga attcgcttag atgccgacaa agacgatgcc tcggttttgc
     5281 atgtgcgcat agagctgacg cagaataaaa agtacgaatt aggtttcgac ctcgacgcca
     5341 attacataga cggctacacc ctcaccggcc ggggcagcct catgggcctg gctttttcgc
     5401 cgacggccca gaaccgcaac ttgtttaaag gcgccgaact gaacatcacc agcctcaatg
     5461 ccggggtgga ggtcaatttt tccaaactca ataccaacgc gttttggaac tcggtagaac
     5521 tggggctgca aaacgagttg tatttcccca aatttgtgga ctacctctac ctctggcggg
     5581 gcatgaacaa gctcccgttc ggtaagaaaa aattggtgag cgacgctttg tatcaggttt
     5641 tacaagacaa ggcggcgtca aggctgtcgg cgcgatacaa ctatgtgttc aacttcgggt
     5701 ggtacgagac cagcctgctc aatgcctcct ttggctacga tgtgcccata gacaacaaca
     5761 cccgcatcgt gctggatcat gtgggaattg actatctgcg ccccgtgacg cagcctgcat
     5821 ttgataccat tttgatggcc aatccgtttt tggcgcgcag tttcggcaag cagttgtttg
     5881 tgagtttgtt cttccgggag ttcaatctgt ctaaacagcg gcgccccaat cgctatggag
     5941 aatcgggctt caacaacctc aacatagaaa tggccggggc cgagatttgg gccggcaatg
     6001 cactgtacaa cgctttcgcc ctcagtcagg acacccttcg gatttccggc acagattttt
     6061 cgcaatacgt tcgcattgag tgggatacgc gctattttaa agaatacacc cccaatctgg
     6121 gctttgccgc ccgcttcaat gtgggcttgg cgcgggcctt tggcttttct accgacgtgc
     6181 cttatgtgaa gcaatttttt gtgggtggcc ccaacagcat tcggggctgg gcagcgcgtg
     6241 gactggggcc tggcggctat cgggatactt tgtcggaggg gcttaccaat cgcctgctgt
     6301 tttatcagac cggagatttg aaattggaat tcaatgcaga gctgaggttt catgtggttt
     6361 cgaggttaaa aggcgctatt tttctcgatg ccggcaatgt gtggacgctc cgccccgatg
     6421 ccgaccgctg cggatctcag ttcctgctca gccgccgcat gtctgaaaac tgcccggtca
     6481 acctgccttt taatgatcca ttttacaaac aaattgccgt aggcacgggg gcgggctttc
     6541 gtttggattt caaatatttc attttccgtt tagatttggg ggtgccgctc aggtacccgc
     6601 gtccggtgag gaatctgatg gatacatcgc cttcggaggc agattattgg cacgatttca
     6661 gcaactggcg gctgcgcgat gtgaatttta atttcgggtt ggggtatccg ttttagaaga
     6721 aaagagcgct caatcacggc tgcaccgaag ctccgcattt cgacttctgt ggcagactgt
     6781 cgtacaaatt gcacaaggat tgaaacgaat accggcagag cttgttaact ttgcggatag
     6841 ttttgtcggc cccggcagca ggaaatctag tccaatctct ttcttcctct tccgttccgt
     6901 caaaatgccg gtgttttcca acgcttcatt taaatatcat tcaaactatg ttgagagcag
     6961 gaattttact tctcttttcc ctctgggctg tgctgtctgt gcaagctcaa catcgccatc
     7021 acggctactg cggcaatggc gaaggcggcg aatgggacgc catgctgacc gaacgattgc
     7081 tgcgcaacaa gcaagcgctt gaagatcagc cggtgcaatt cagaagcacc atttatgtgc
     7141 ctttgcgcat tcacatggtg gccaacagca gcggttttgg gcgcgttacg cacgcccagg
     7201 tgctggacca attgtgcaaa ctgaacgaag attttgccga catggacgtg cagttttaca
     7261 tcaagggcct caacgacaac atcaacaaca acggcattta taccacccag tacagcgccg
     7321 gtttggtgat gaacaacctc cgggatgcca ctgccctcaa catctggata gtggacgtgg
     7381 cagcaggctc tcagccttcg cccggcgacc tcggcgttac gctcggctat tactcgccga
     7441 cacgcgactg ggtcgtcatt cgtcgcgatg aggtgaacag tagttccgtt accatttctc
     7501 acgaaatagg tcatttcttc agcctcaaac acacacacaa cggctgggac agtacgccct
     7561 ggtcgcccac catcggcaac cccgcgcctg tcaattcgcc cggcggcgtg cccaccgaac
     7621 gacaggacgg cagcaattgc aatacggccg gggattatat ctgcgatacc ccgccggatt
     7681 acaacggctt tggattcaat ggctgcaatt acaacattgc tcaagacccg accggcgcct
     7741 tcatcaatcc tgatgagcaa ctttttatga gttattttct caactgcgtc cgcaacgact
     7801 actatttttc gcctacgcag caaaacctga tgattgccga ctacaacagt acggcccgca
     7861 actacctgcg cacttccgcc gtgacgccca acctgacgca gatcacctcg gctgcaacgc
     7921 ccgtttcgcc cattaataat gattttacgc ccggttacaa catcgtcaat tttcaatggg
     7981 cggcagtgac cggcgctgct ttttaccttc tggaaacaga cgtttctgcg gccttcaaca
     8041 gcaacccgac ccgcgttgtc gtcagcggcg ccacctcgca taccatgaca acgctgctgg
     8101 ccaatcgtac ctactactgg cgggtgcgcc cttacaacgc ttaccgcacc tgcgcgcctt
     8161 tctctgcaac agcgcagttc agaaccaaca atatgaccgt gagtgccgat aacattgagg
     8221 aactgaacca atggtcggta tttcccaatc cattggcgga agatggccgc attcaggtgc
     8281 aattgcaggc tgcgcaaggc ttcgaaggtc ggtttttgct gtacgatgtg gccggacgta
     8341 tggttcgcag catgggcagt catcgcatat cgggcggcta tactcaattg gagttggacg
     8401 cgggcggctt gctccccggt gtttacacgc tgacgctgga acacgaaaat ggccgcgaac
     8461 tgaggcgcat tacggtggca aagtgattca attcaacctt ttcaaactac ttttttcaat
     8521 gaagaaatta attttatcgg ctgtttttat gatttttgga atggccgtat tgcaagccca
     8581 gaccaacacc ggcccttgcg gcaacggcgg cggtgagtgg aaagaaatga tcatagagcg
     8641 gctactcaga aacaagcaat tgctggccga agaccctgtg cagttcaggg atgtgattta
     8701 tgtgcccgtg cgatttcact tggtggccaa ttccagcggt accggccggg tcgtattcgg
     8761 gcgggtattg gaacagcttt gcaaactcaa tgaggattat gccgattttg atatgcagtt
     8821 ttatatcaaa ggagtcaacg acaacatcaa caacacggcc atttacaatg cgcaatacaa
     8881 cgccgggttg gtgatgaacc agttgaaaga ccccaatgcg ctcaatatct ggattgtgga
     8941 tgtggctgcc ccggctgcac cttctccggg cgacgagggg gtcatattgg gttactataa
     9001 ccctggccgt gactgggtag tggtacgtcg cgatcaggta ggcgcttctt ctgtgacgtt
     9061 nnnnnnnnnn gaagtaggcc acttcttcag cctcgaccat acgcacaacg gttgggacag
     9121 cactccctgg tcttcttcta tcggcaatcc ggccccgctg ttttcccccg gcggcgtacc
     9181 tacagagcgt caggacggca gcaactgcga aaattcaggc gactttatct gcgatacgcc
     9241 gcccgactac aacggctttg ggttcaatgg ctgcaactac aacattgccc aagaccctgc
     9301 cggcgttttt atcaatccgg acgagcaatt gtttatgagc tacttcctga attgcacccg
     9361 caataactac tacttctcgc ctacgcagat caacctcatg atgacggact acaaccactc
     9421 ctcccgcaat tatctgcgca ccggagcagt tacgcccaat ctcaccgaaa ttaccgaggt
     9481 gccgacgctt actttcccca tcaataacga aaccacgccg ggttacaaca tcgtcaattt
     9541 ccagtggacg gctgtggccg gcgccgattt ttaccttttg gaaatagacc gtgtggccac
     9601 ttttacgctc aatccgatcc gaatcattgt agccggagcc aactcccgca gcgtcaccac
     9661 tttagaggcc aatcgcacct acttttggcg ggtgcgcccc tatagcgcat accgcacctg
     9721 cgcgttgtcc acgcccgtag ccactttcaa aaccaactct accgtggtag ccacccagga
     9781 aatcgaagca ttgaacgagt ggacggtatc gcccaatccg attatatcac aaggggcgct
     9841 tcaaatcagc ctccaggccg agaagtcatt tgatggagat ttcagtctgt tcaacgcagc
     9901 cggacaactg gtgcgcagcc tcggacgtca ccgcgttcag tcgggcgcca catatttgga
     9961 attcagcatg gaagggctgt cttccggcgt atatatgctg gcgctctcac acgaggaagg
    10021 ccgggaaata cggcgggtgg tgctggcaaa gtgatatttt ctcgcccctt attgctgaaa
    10081 aaggcgcttc atcgaggtat gaggcgcctt tttttgtgtg attgcttgct gattaaaatc
    10141 atagcgatag atggaaaacc aatctatact gcgggactgt ccattgaccc gcatcgcact
    10201 cctcacgcgc ctacttcctg gaaatctcgt accttcgtcc cgtaaatatt tccaatgagt
    10261 taccatccgt cagaactgcg ccgagtgaac atcatccgct atgtgacgcc cttgagggag
    10321 ggcggttcgc tgcctgccat tgtggaggcg gacgatgatt tcctgtatgt agcgaagttc
    10381 aggggtgccg gacagggcac aaaagcgctc attgccgacc tcatcggtgc agagatggcc
    10441 cgcgttgccg gccttcaggt acccgaactc gtgctggccg acctcgacga gtcatttggc
    10501 cgcacagagc cggacgagga aatccaggac ttgctgaaat tcagtaccgg caccaatctt
    10561 ggcgtccatt acctttccgg ggccatcagt tatgaccctg tggtgaataa agcagaaccg
    10621 ctgacggcct cgaaaatcgt gtggttggat gccttgctca ccaacgtgga ccgcacggcc
    10681 agaaacacca atatgctcgt ctggaaccgg gagctttgga tgattgattt tggggccggc
    10741 ctttattttc atcatagttg ggacaattgg gagcagcaat cacagtcggc attcccccat
    10801 atcaaaacac atgtactcct gtcgcaggcc gctcaattgg acgaagccga cgaactgctg
    10861 cgccaggcca tcacgtccga aatcatcgcc gccatactgg ccgccatccc cgatgattgg
    10921 ctgcacggac atggttcgcc cgacgaaatg cgcgcggttt atcatgattt cctgacccgc
    10981 cgcatcgccg catcttccac ctttttaaac acagccaaag atgcaagagc gggaattatt
    11041 tgaatacgca gtcatccgtt gggtgccgcg ggtagagcgg gaggagttcc tgaacgtggg
    11101 cgtgattttg ttttgcaaaa agcgcaactt cctgcatgtg ctgtacgaag tgaaggaaga
    11161 tcgcctgaaa gcccttttcc cggaatgtga gttggaagag gtcaaatgcc acctggatgc
    11221 ttttgcccgc atcgccgccg ccgatacatc cgccggatcc atcgcccaat tggatgctgc
    11281 cggtcgtttc cgctggctga cggcacagcg cagcacggtc atccaggcct cgaaagtgca
    11341 tccgggctta tgcacagatc cgatggagat gttggaaagg ttgtttgggc agatggtagg
    11401 ctaatctgga gagaaccggg cacgagcggc aaaggccgga gacagggaaa tagggtacct
    11461 gaatttggcg aggtgaggaa aaggctggat aattttcact ttaagtgctt ctttataacc
    11521 gattcttcac ggagtacatc tattaactgg caatatggaa tttcaatcct cattccgcct
    11581 cctccaatgc cattgtgttc aaatcgctga taaagaaaaa ctatcccttc tttagctata
    11641 taaaaattgg tcgaaatagg aatatattct ccgttgatgc cattctcttt gagggaggtt
    11701 atgtgaaaat gttgtttgta ttgattggtt aatttggctt gaagcgtatc caatttgttg
    11761 ctgtcaaata attcttcgaa tgaaatcttt tttccttttt caaaatcata gttaaatccc
    11821 acattgttgc catatcctat tgcccctccc gatttgtgtt caaagtcgct atgtattgca
    11881 gaaatgatat gagggctatt gattatcaat attacttctt cccaatcagt ccagctatgt
    11941 ttttcatcct caggtgtctt tttaaaatac gtttgatatg ctttttcatt agtagaaaaa
    12001 taagtttttg aatccgattt tttgtcaaaa accataagct ctatttcttt gttgagctga
    12061 cttttttttc cggtgctttc ataaaattgt ggataagaaa actcagcttc gtatccttca
    12121 cctttttctt gaatttttac cattttataa atcagggtat cttcggttaa tgacaatttt
    12181 aaaaggcatt cgggaatttg ttccatttca ctgccttttt cctgaaattc agcgatccgt
    12241 tcgttccagg ttctcaactg aattttctgc ctgtaagtat caccggtgta aatcaaaacc
    12301 aattcaccga cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn acaccagcac gccaaaaaca tttcaccccg caatcccccg
    12481 cttcagcccc gccaccaaac tcacatccgt ttccatccag ttgaactcct catcggcgga
    12541 ctcgtcgccc atgttgatgg cgccccggta ctgcatttcg ctgcgaacga cgccgcgcac
    12601 cgctgcatga aactcttgta tttcaggcac ttccgtcagc gggccaacat tatcgggcat
    12661 cagactgccg caggccatga tggagaggcg accggcagcg tattgcgcaa agcggcgcag
    12721 atgctcgaag cccagcgcgg cattgggttg ctgccccgaa ctcaggatgc gctgcacgcc
    12781 caattccgcc aactcgtcta tggcgcgcag cgggtcgcgg cacatatcga aagcacggtg
    12841 aaaagtgaag ggcagaccct cggcggcctc caacatgagg cggatgcggg ccaggtctat
    12901 gttgccgtcg ggcaggagcg cgcccgaaac gatgccgtcg gcgcccagtt cgtgggcgat
    12961 acgagcggcg gaccacttgc ggctccgccg cagtgtctcg atccgcgtta cggtctcggc
    13021 cggagtcgcg gtgggctgat ggtgcggaac gctgggacga tcgagcagac cgagttcgcc
    13081 gtgttcgcgg tagcgggcca tccatttgct ggcgcaggcc cgggagatcc ccatctcggc
    13141 ggcgacatgg gagagcgggc gggtctggca gcgctcgacg agacggcggc gaccttcgac
    13201 ggacagcggg gcgttacggt ggagcacgga cgggttcttt cgttcggcgg atgag
//
LOCUS       DAORLF010000063        13234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2508_length_13234_cov_1.942560, whole genome shotgun sequence.
ACCESSION   DAORLF010000063 DAORLF010000000
VERSION     DAORLF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_13234_cov_1.942560"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    155..171
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<172..367)
                     /locus_tag="PK198_07395"
     CDS             complement(<172..367)
                     /locus_tag="PK198_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38595.1"
                     /translation="MLKPIQTLALSTLSLLMCLLPAGAAAEYLVFPVERYISLNTAQL
                     VAYEYWFDYTGQPVFVPISPT"
     gene            complement(436..1437)
                     /locus_tag="PK198_07400"
     CDS             complement(436..1437)
                     /locus_tag="PK198_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38596.1"
                     /translation="MRIITMFLGICLTVLTFSSLQAQKLIALHHEGAVSYFTELSAAY
                     NSSVSGDTLYLPGGSFGGLSLQKSLTLIGVGHHPDSTAATGATILSYLHLYSSASNSL
                     ITGIYFISSIESYESLSNITISRCYLNQGIYLGNGEKSNWIISENWIGVYPYPNGSYS
                     IWLQSGSASNFFVSNNVIRRIIAVSYSAISNNIFLDNAFVSTTNSIISNNIFSTNYQN
                     QAGSSQWFNNVNSGINGGPNISGNNGSSNYLDNLALESIFVNYSPSNTFYQNDFHVVN
                     SAYLGNDGMPVGIYGGAFPWKDGSLPFTPHIRSKTIGATTNPDGTLNINITVKAQGN"
     assembly_gap    1984..1999
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2000..2529)
                     /locus_tag="PK198_07405"
     CDS             complement(<2000..2529)
                     /locus_tag="PK198_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38597.1"
                     /translation="METNHEVINAWAEMIGAIAWPLVVLVLFPFLLKLLHSWVVGYGK
                     EIAFSSGKISLVVKPTEASRPPANATPEEKALRAGEVQNIEASPGKALPADYFFLNHT
                     SFLRPDKQEEFQKRTGIARKHYDIQLIVDSYYRDALQRIEYVEYLLHRAYPHPVQYRR
                     SAKDHFMLKELANGEYV"
     gene            complement(2605..3309)
                     /locus_tag="PK198_07410"
     CDS             complement(2605..3309)
                     /locus_tag="PK198_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HRF38598.1"
                     /translation="METSEKTIVKEVPAIKTFEGYLFAKLNAIGSKSEGPSYYLQQHD
                     GKEIPVVKRVAPWMKYPLLHACVGQKVALLGKMTKVAVAEKSGIHCGYVLNLVEPLVM
                     GLDIDLKEDTLWINKMPGPAMEYPPQMKSIKIKLSVTWPFRSIWHGECPSSQLFDFGI
                     ENPEGKTIWRWGQCMMFKPEPTKVQIPGASPKSVTAPWQYFEDAITVEGLYVAKAEFI
                     ASGQVIRKPFWVKFPQ"
     assembly_gap    3481..3500
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3501..3929)
                     /locus_tag="PK198_07415"
     CDS             complement(<3501..3929)
                     /locus_tag="PK198_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38599.1"
                     /translation="MHFAWYLEPDSKKFDSAASYLWVCAGPSTALGAKRNWYPSVLPR
                     EARRDNLLFITSNNKAHFAWYQKGDALWVGKGSSDNLAKEKAVRCSLPPDAKVGFILF
                     MSTNDQHHFAFLKNGTYLVGSSEKLAHSPQGVYDLEELKDI"
     assembly_gap    3957..3974
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4257..4826)
                     /locus_tag="PK198_07420"
     CDS             complement(4257..4826)
                     /locus_tag="PK198_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38600.1"
                     /translation="MLGDAICASVCTNATSQEVSTPNFENQKIKAMAKYGQILNPDRF
                     RSQHWTHTRTGDGKELITITGSVVIDFKMTPPYGGWTKETLELELMLPIQFPAGKVLH
                     IDQWAPFFTINGFYGDTHATGAGGPTSANHGVIIEDFWGPGRVKTGGSFSVFANIGVS
                     GRQTKFHRIGYHITLVGSFVKADPVRPVM"
     gene            complement(4926..6413)
                     /locus_tag="PK198_07425"
     CDS             complement(4926..6413)
                     /locus_tag="PK198_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38601.1"
                     /translation="MKTIPFALQFDVALCAFLLVCGHILQAQTFENTIGQAQSREIAW
                     DGKPIPGIQKYVVISNSFSGGDTSEGMMLFSRFQNNGKWELSKIYATDREWIKMLNGR
                     AIERGVFTGRKNIVYIAGERKESANKLAFLMIADEDGTPIHAQHHVVTGAKESMTSSL
                     ETINPIWNAIKPGVGSVIATGNILGDFHDKGPKEQFFVAYFKSSLRGGLVWSFRYFDP
                     SSARIGLHVNKTCLGELSEGTKVVVATGYQIMRETRQLFVSCIDLKSGTELWRQVVLP
                     EMPAMTEGFDVVQDPQTRNFVAVGYYHRPNGYKQMYVAVWDQKGSFLGATHYSLAGNI
                     PGDIVARDVICSIDKKSAVVSGYLNSAELSNRPKTFALELPFTPLAANPLWAKYYERS
                     KPDPMATESIQLGISTGRQAGYFITSQANKLSIQPETYGIHIIHTDQTGNTLMSDPHC
                     AVLPFQLNHARGDKAMPLERKQEETRWKSFQVFYKEVTLQEQPCR"
     assembly_gap    6502..6511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6644..>8158)
                     /locus_tag="PK198_07430"
     CDS             complement(6644..>8158)
                     /locus_tag="PK198_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRF38602.1"
                     /translation="FLEYIRQAPGKQPVAERLQHYGEFTKMYDTVELHHQSARCMDCG
                     VPFCHNGCPLGNIIPEFNDAVYRGRWQEAYEILASTNNFPEFTGRICPAPCESACVLG
                     INQPPVAIEEIEKHIVEIAFERGLVKARRIFHRTGKKVAVVGSGPAGLAAAAQLNAAG
                     HSVTVFERDDAPGGLLRYGIPDFKLEKWVVDRRIALMEAEGVEFRCSTEVGSAALPAG
                     DLFREYHAVVLCIGSTIPRDLPAPGRDVVQGVHFAMDFLKQQNKRVRAGQPFDLEKTA
                     ISAKGKHVVVIGGGDTGSDCVGTSNRQGAASVTQFELLPEPPKDRAAYMPWPTYPMLY
                     KVTTSHEEGCERRFAVQTKAFLGDENGQLRALRIVDLEWAAGLDGRPSSFVEVPGSER
                     EIPCELALLAMGFLHPQQEGLLAQLELATDARKNISAPEHSYQTNIPRILFRRFASRA
                     VSGCMGHTGRAGMCAECGCVFEEMREGVKFLKSGKCAIQDEPDFDNSLLRFLFK"
     assembly_gap    8159..8175
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8183..>8499)
                     /locus_tag="PK198_07435"
     CDS             complement(8183..>8499)
                     /locus_tag="PK198_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38603.1"
                     /translation="RTGRNFGAGMSGGIAYVWDVKGDFAERCNLEMIDLDPLDEADVA
                     QVRAMLERHYRFTQSSVARFLLSDLEQQAGRFVKVFPKDYKQALARRAAHSITQNTER
                     HG"
     assembly_gap    8500..8601
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8602..>8919)
                     /locus_tag="PK198_07440"
     CDS             complement(<8602..>8919)
                     /locus_tag="PK198_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38604.1"
                     /translation="VNTDRAIGTMLSNEITKRYRAAGLPPNTIRFKFRGTAGQSFGAF
                     SAAGLSLELEGDANDYFGKGLSGARLVVYPDREARFVPEENSIVGNVAFYGATSGEAY
                     IRGK"
     assembly_gap    8920..9134
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9135..>9470)
                     /locus_tag="PK198_07445"
     CDS             complement(<9135..>9470)
                     /locus_tag="PK198_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRF38605.1"
                     /translation="HQTLVRNKLRSRVVVQADGQLKTGRDIAIAALLGAEEWGVATAA
                     LVVEGCILMRKCHLNTCPVGVATQDPELRKRFSGDPDHVVHFFKFLTQELREIMAQLG
                     FRTVEAMIG"
     assembly_gap    9471..9602
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9603..>9921)
                     /locus_tag="PK198_07450"
     CDS             complement(<9603..>9921)
                     /locus_tag="PK198_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRF38606.1"
                     /translation="IKQVASGLFGVTSLYLSEAEELQIKMAQGAKPGEGGQLPGHKVD
                     EWIGKVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSKAGVGT
                     IAAG"
     assembly_gap    9922..9949
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9950..>11168)
                     /locus_tag="PK198_07455"
     CDS             complement(<9950..>11168)
                     /locus_tag="PK198_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRF38607.1"
                     /translation="QLFAQVTNPPIDPIRERTVMSLATFMGNIGNILEEDPEVCHTVA
                     LPHPVMTNHELEKIRSIDTGIFQAKTLQMYFRADGKPGSLKKALDRVCRYAVDAVEDG
                     FEVLVLSDRAIDSEHAAIPSLLFTSAIHHHLIRKGYRGKVGIVVEAGDVWEVHHFACL
                     LGFGATAINPYMALAGIRDLHQDGRINPELSAAKLEQNYIKAVKEGLLKVFSKMGIST
                     LQSYMGAQIFEIIGINRKVVDQYFTGAVSRIEGMGLDEIARETLMKHQWAFGAGTEEG
                     SRLPEGGIFQWKRRGEVHLFNPQSIHLLQYATRQNDYATFKKYSKVIDDQSEKAVTLR
                     SLFEFNSQRPPVPIEEVEPAENIYRRFATGAMSFGSISWEAHTTLAIAMNRLGGKSNT
                     GEGGEDESRYEPLP"
     assembly_gap    11169..11188
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11189..12747)
                     /locus_tag="PK198_07460"
     CDS             complement(<11189..12747)
                     /locus_tag="PK198_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRF38608.1"
                     /translation="MSRQHGQGLYCADMERDACGIGFIANLRNNKNHQTVSDALTILE
                     NMEHRGACGCEENTGDGAGIAVQIPHAFLFDECLRLGVRLPEPERYGVGMAFFPKDLR
                     RREACREVIVRSAERLGLEVLAWRKVPVNPAGIGETALSVEPEIEQVFIARPDTISNA
                     EEFERKLYVLRNHASHMAAAGIPSDDPVGLYFASMSCRTLVYKGQLTSLQLRYYYPDL
                     QDARFVSAFGLVHSRFATNTFPSWKLAQPFRFIAHNGEINTIQGNLNWLKTSEKSLQS
                     ASFSREEMEMLLPVVSGGQSDSAYIDNMIELLTLSGRSLPQVMMMLIPEAWDGNPHMA
                     AYKKAFYEYHASFMEPWDGPAAICFTNGQLIGATLDRNGLRPARYLITHDERVILASE
                     TGVLPLPPDIVREKGRLQPGKMFLVDLVQGRVIGDEEIKQEICTARPYAEWLEKYKIR
                     IEELPDPRVTFAPLEPAQVFKYQQAFGYTSEDLEDILLPMALDGKEPIGSMGTDIPLA
                     ILSDRPQHLSY"
     assembly_gap    12996..13019
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2853 a         3283 c         3292 g         3187 t
ORIGIN      
        1 cataagctac aatctgtcgt tccccggctt gctcaggagg cgttttgacg aagaactggc
       61 tcagtacact gctccaggcc ccttgatcgt ccagaaagcg gatgtgcagc gtatgcaagc
      121 ccgctgtaag gtcggttgta ggtaagtcca taccnnnnnn nnnnnnnnnn ncggtcggac
      181 tgattggaac aaagaccggc tgcccggtgt agtcgaacca gtattcgtag gcgaccaact
      241 gggcagtatt caaagaaata tatcgctcaa ccggaaagac caagtattcc gcagcggcgc
      301 ctgccggtag caggcacatc agcaggctga gggtggagag cgctaaggtt tggattggct
      361 tcaacatggc aaaggagttt aaaatcagga tgcagcgttg cgatcatcag atcatgttgt
      421 tcatgtcttg ctgctctaat tgccctgtgc ctttaccgtg atgttgatgt tcagtgtgcc
      481 gtccggattg gtggtagcgc ctatggtttt tgaccggata tgaggcgtga acggcaggct
      541 gccgtccttc caggggaaag cccctccgta gattcccaca ggcatgccgt catttcccag
      601 ataggcggaa tttactacgt ggaaatcatt ttggtaaaaa gtattgctgg gcgaatagtt
      661 gacaaatatt gattccaacg ctaagttatc gaggtagttg ctggagccgt tattaccgct
      721 gatgtttgga ccgccgttga tacctgagtt cacgttgttg aaccattggc tgcttccggc
      781 ttgattttgg tagtttgtgg agaaaatatt gttgctgatg atagaattgg ttgtagaaac
      841 aaatgcatta tcaagaaaaa tattattgct aattgccgaa tatgaaacag caattatacg
      901 tcggattacg ttatttgaca caaagaagtt acttgcactt ccactttgga gccaaattga
      961 ataactacca ttgggatagg ggtaaactcc tatccagttc tcagaaatta tccagttaga
     1021 tttttcacca tttccaaggt atattccttg atttaaatag catctggata ttgtaatatt
     1081 tgacaaactc tcatatgatt caatgctgct tatgaaataa atccctgtaa tcaaactatt
     1141 actagcacta gaatacaaat gcaaatatga aagaatcgtc gctcccgtcg ccgcagtgct
     1201 atccggatga tgccctacgc ctattaaggt caatgatttt tgcaggctta atcccccaaa
     1261 gctccccccc ggcaaataga gcgtatcacc gctcactgag gaattgtatg cggcgcttag
     1321 ttcggtaaag tacgaaaccg ccccctcgtg gtgcagggcg atgagttttt gggcttgtaa
     1381 ggacgaaaac gtcaggactg tcaaacaaat gccgaggaac atagtaatga tacgcataag
     1441 actattggtt ttaaaagtga ataaaacgca tgttatgatc ttatcaccaa atgtactcca
     1501 catacctgaa ccttcgccgg aacgcataca aatgctacgc cacagcacac atggtgaacg
     1561 gttagtgagg tgttctttcg cagaatggtc ggttgaaaaa tgtattgcca gggtcaatat
     1621 ggcaacatga gactgcaaga taagatgatt ttgggatgtg ggcgccaacg atcgttcttt
     1681 tgacaaaaaa atgcctgaat atcgcttatt tatggggagc tgctctatta caggcgctat
     1741 cattccgggc ttggcgatca ggtcatcaaa tatacacgta tgtgacaacc actctccggc
     1801 tcattgcccg atgtcctcga ctcaacaaag cggaccatag cacctgccgc ttcctgtctt
     1861 cccttactac taagtgtgtt agacaaactc cctcaatgct cgaccttatt atccaaacga
     1921 ggaccacttt cccatagcga aatataccgt tgcagcacca gcggctcttt ggcgtctttc
     1981 aggnnnnnnn nnnnnnnnna catattctcc attagccaac tctttgagca taaagtggtc
     2041 tttggcgctg cgacggtatt gcaccggatg tgggtaagct ctgtgcagca gatattccac
     2101 atactctatg cgctgcaagg catcgcgata gtaggagtca actatgagct gaatgtcata
     2161 gtgcttgcgt gcaatgcccg tccttttttg aaattcctcc tgtttatccg gtcgcagaaa
     2221 agaggtatgg ttcaggaaaa agtagtctgc aggaagcgct ttgccgggag aagcctcgat
     2281 gttttgaact tcacctgccc gtagggcttt ctcctccggt gttgcattgg ccggcgggcg
     2341 tgaagcctct gtgggtttta caaccagcga tatttttccg ctgctgaagg cgatttcttt
     2401 cccatagcca acgacccagc tatggagcag cttgagcaaa aacggaaaca gcacgaggac
     2461 aaccaaaggc caggctattg cgccgatcat ttctgcccat gcgttgatta cttcatgatt
     2521 tgtttccatg atgatggttt ttgtttgatc agattgctgg tggcatcgca ggctcctccc
     2581 gactcaggta ccgatggcgc attatcactg cggaaacttc acccaaaacg gcttccggat
     2641 cacttgtccc gaagcgataa actccgcttt agcgacgtaa agcccttcca cggtgatggc
     2701 gtcttcaaag tattgccagg gtgcggttac cgacttcgga gaagctccag ggatctgcac
     2761 tttggtaggc tccggcttaa acatcataca ttgaccccat cgccagatcg tctttccctc
     2821 tgggttttcg ataccaaagt cgaatagttg cgagctggga cattctccat gccaaatgct
     2881 acggaatggc catgtgaccg acagtttaat tttgatgctc ttcatttggg gcgggtactc
     2941 catggctggg ccgggcatct tgtttatcca aagcgtgtct tccttcaagt caatatccag
     3001 tcccatcacg agaggttcga cgaggttgag cacataccca caatggatgc cacttttttc
     3061 ggctacggcc actttagtca tcttacccaa caaggccact ttttgtccta cacaggcgtg
     3121 cagcaaaggg tacttcatcc aaggagctac tcttttcaca acaggaattt ctttaccatc
     3181 atgttgttgt aagtaatagc tcggtccttc ggattttgaa cctatggcat tcagttttgc
     3241 aaacaggtag ccctcgaatg tttttatagc agggacttcc ttaacgattg ttttttcaga
     3301 tgtttccatg gtatttgatt atttggtgat tgtcaaactt cttgtttctt ctcagcaagc
     3361 caagtctcgt cactcccaaa tgaattcatt caggctcttt ccctcatcaa gttgccaagt
     3421 gcatgcgaat gcccttgaaa aaatatagct acatgagtgg aggctaagat tcttacactt
     3481 nnnnnnnnnn nnnnnnnnnn tatatccttc agttcttcca aatcgtacac tccctgaggg
     3541 ctgtgcgcta atttttcact gctccccacg aggtaggtcc catttttcag gaaagcaaaa
     3601 tggtgctgat cgttggtgga cataaagagg ataaagccca cctttgcatc tggcggcagc
     3661 gagcagcgaa cggctttttc ttttgccaga ttgtcgctgc tgccttttcc tacccaaagt
     3721 gcatcgccct tttgatacca ggcaaaatgc gccttgttat tgctggtgat gaacagcagg
     3781 ttgtcccggc gtgcctccct aggcagcaca gatggatacc agtttcgctt ggcgcccaat
     3841 gctgtagatg gaccggcaca tacccacagg tagctggctg cactatcaaa cttctttgaa
     3901 tccggttcga gataccaggc aaagtgcata gtgtcattgg aggaaatgct aaataannnn
     3961 nnnnnnnnnn nnnngtgctt tcccttcaaa aggcactatg gtaaaaggct gacgcggtct
     4021 attggcatcg tgattccggg tcgtaccggc actcacttcc tgggtatccc accaggcgaa
     4081 gtggtacttg ctgttggcgg agagaaattt ggctttcatg atgaatgatt gttggagtga
     4141 tgtaattgat tgaatatggt tatgaacccc tcggaaaata ttatccaatt ccaagattgc
     4201 acactgataa tcaggcaaaa agaaaatcag cagtcctgtt ggttgcattt tcttaactac
     4261 atcacaggtc ttacaggatc agccttcaca aaggatccta cgagtgtaat gtggtatcca
     4321 atccggtgaa acttggtttg tcggccggac actccaatat tggcaaaaac agaaaaacta
     4381 ccacctgtct tcactcttcc cggtccccag aaatcctcaa taattacccc atgattagcg
     4441 gaggtcggcc ctccggcacc tgtggcatgg gtgtctccat agaatccgtt tatggtaaaa
     4501 aatggcgccc actgatcaat atgtaaaacc ttaccggctg gaaattgaat gggcagcatc
     4561 aattccagtt cgagtgtttc ctttgtccac cctccatatg ggggggtcat tttaaaatca
     4621 atgaccacgc ttcctgtaat ggtaatcagt tcttttccat ctccggtacg cgtatgcgtc
     4681 cagtgctgag accggaacct gtccggattc agtatttgtc cgtattttgc catggctttt
     4741 attttttggt tttcaaaatt tggtgtgctt acttcttgtg aagtagcatt tgtgcataca
     4801 cttgcacaga tggcatcccc caacattttt tttcggtcat acatgcacct tgttggaggc
     4861 tttttcagct tatctctcgg ttatggtcaa cgctttgccc tgaaaaagct gaacctgaaa
     4921 agacttcacc tgcaaggctg ttcttgcaag gtcacttctt tataaaagac ttgaaatgat
     4981 ttccacctcg tttcttcctg ttttctctca agcggcatgg ctttgtcgcc tcgagcatga
     5041 ttcaattgaa agggtaacac agcacaatgt ggatcactca ttagagtgtt tcctgtttga
     5101 tcggtatgta tgatatgaat gccataggtt tccggctgaa tactcagctt atttgcctga
     5161 gaagtaatga aatagcctgc ttgccgccct gtgctgatgc caagttggat gctttctgtt
     5221 gccataggat caggcttgga tcgttcatag tactttgccc ataaagggtt ggcggccaag
     5281 ggagtgaacg gcagttcaag tgcgaacgtt ttcggtcggt tgctcaactc agcactgttg
     5341 agataaccgc tgacaacagc cgactttttg tcaatactgc aaatgacatc ccgggcgaca
     5401 atatcccccg gaatattgcc ggccaacgaa tagtgtgtgg ccccaaggaa actgcctttc
     5461 tgatcccaaa ctgctacata catttgttta tagccgttgg ggcgatggta atagcccaca
     5521 gccacaaagt tccttgtctg cgggtcttgt acaacatcaa aaccttcagt catagccggc
     5581 atttccggca gtactacctg ccgccaaagc tccgtgccgc ttttcaagtc aatacaagag
     5641 acaaaaagct gacgggtttc ccgcattatc tgatatcccg ttgctactac cactttcgtg
     5701 ccttcggaga gttcgccaag gcaagtttta tttacatgca aaccgattct tgcagaggag
     5761 ggatcgaaat aacgaaaaga ccacaccagt cctcctctca aacttgattt gaaatatgcg
     5821 acgaaaaact gttccttcgg ccctttatca tgaaaatcac caaggatatt tccggtcgct
     5881 atgacacttc caaccccagg tttaatggcg ttccagatag gattaatagt ttccagagag
     5941 cttgtcatag attctttggc gcctgtcact acatggtgct gtgcatggat aggcgttcca
     6001 tcctcatcag caatcatcaa aaacgccagt ttgttagcgc tctctttgcg ttcgccggca
     6061 atatatacta tgtttttccg tcctgtgaat acacctcttt caatagctct gccattaagc
     6121 atttttatcc attcccgatc cgtagcatat attttggaaa gctcccactt accgttgttc
     6181 tgaaaacggg aaaacagcat cattccctca ctggtatccc cgccggaaaa agagttcgat
     6241 ataacaacgt acttctgaat accgggaatg ggctttccat cccaagcgat ttcccggctt
     6301 tgagcctggc cgatagtgtt ttcaaatgtt tgagcttgta aaatatgccc gcacaccaaa
     6361 aggaaggcgc ataatgccac atcaaattga agggcgaaag gaattgtttt catgagaaat
     6421 attttgggat tgatcataac ctgacttcag gctttgcttc tatgcccaaa ttgtactttt
     6481 cccatgagag cagcaagagc cnnnnnnnnn nggatcatcg gcggttgata caggatgacc
     6541 ggtaaataca agcgtaatac ggtaactcat tcgcgactgc tcacgccgca gtattcgtca
     6601 tacgaatatg cgtatttcgg aatcgaaaaa taatcctggt tttctattta aacaggaatc
     6661 gtaataagct gttgtcgaaa tcaggttcgt cctgaatagc gcatttgcct gattttagaa
     6721 atttaacacc ttccctcatt tcctcaaata cgcatccaca ctccgcgcac attcccgccc
     6781 ttcctgtatg gcccatacaa ccagagactg ccctcgacgc gaatcgccgg aacaaaatac
     6841 gcgggatgtt ggtctggtac gagtgctccg gcgccgaaat gttcttgcgc gcatcggtgg
     6901 ccaattccaa ctgagccaac aggccctctt gttgagggtg caaaaagccc atcgccagca
     6961 aggctaattc gcaggggatt tcgcgctccg agccgggcac ttccacaaac gaagaaggcc
     7021 gcccgtccag ccctgccgcc cattccaaat ccacgatgcg gagggcgcgc aattgcccgt
     7081 tctcgtctcc taagaaagcc ttggtttgca cggcaaagcg gcgctcgcag ccttcctcgt
     7141 gcgaagtcgt cactttgtac agcatcgggt aagtaggcca gggcatgtag gccgcgcggt
     7201 ctttgggagg ttcgggcagc aactcgaact gcgtcaccga tgcagcgccc tgccggttgg
     7261 aagtgcccac gcaatcgctg ccggtatcgc cgccgccaat gacgacaacg tgtttgccct
     7321 tggcggaaat cgctgttttt tccaaatcaa aaggctgccc tgcccgcacg cgcttgtttt
     7381 gctgtttcag aaaatccatc gcaaaatgca cgccctgcac cacgtctctg ccgggagcgg
     7441 gcaagtctcg gggaatggta gaaccaatgc acaacacaac tgcgtggtac tcgcggaaca
     7501 ggtcgccggc gggcaaggcc gcagaaccca cctctgtact gcacctgaac tcgacgccct
     7561 ccgcttccat cagcgcaatg cggcgatcca ccacccattt ttccaatttg aaatcgggga
     7621 tgccgtagcg caagaggcct ccgggcgcgt cgtcgcgttc aaaaaccgtg acggaatgcc
     7681 cggctgcgtt cagttgggcg gctgccgcca aacctgccgg accggaaccc accacggcta
     7741 cttttttgcc cgtgcgatgg aagatgcgcc gggctttcac caagccgcgc tcgaaggcga
     7801 tttctacgat gtgtttttct atttcctcaa tggctaccgg cggctgattg atgcccagca
     7861 cgcaggcgct ctcgcagggc gcagggcaga tgcggccggt gaactccggg aagttgttgg
     7921 tggaggccaa tatttcgtag gcttcctgcc agcggccccg atacacggca tcgttgaatt
     7981 cgggaatgat attgcccaat gggcagccgt tgtgacaaaa aggcaccccg cagtccatgc
     8041 acctggccga ctgatggtgc agttccaccg tgtcgtacat tttggtgaac tctccgtagt
     8101 gttgcaggcg ctccgctacg ggttgtttgc cgggcgcttg ccggatatat tctaaaaann
     8161 nnnnnnnnnn nnnnngcttg gattagccat gacgctctgt gttttgagtg atggaatgtg
     8221 cggcccggcg ggcgagggcc tgtttgtaat ctttggggaa taccttgacg aagcggccgg
     8281 cctgttgctc cagatcgctg agcaggaagc gcgccaccga actttgggta aaccggtaat
     8341 gccgctccag catggcccgc acctgcgcca catctgcctc gtcgagtggg tcgaggtcaa
     8401 tcatttcgag gttgcaacgc tctgcaaaat cgcccttgac atcccacaca taagcaatgc
     8461 cgccgctcat gcccgctccg aaattgcggc ccgtgcgccn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn ncttgccccg gatgtacgcc tcgccggagg tggcgccgta
     8641 aaaggccaca ttgcccacga tgctgttttc ttcgggtaca aaccgcgctt cccggtcggg
     8701 atagaccacc aaacgcgcac cgctcagtcc cttgccgaag tagtcgttgg cgtcgccttc
     8761 caactccaat gagaggcccg ccgcagaaaa tgcgccgaaa ctctggccgg ccgttccccg
     8821 gaatttgaat cggatggtgt tgggcggcaa accggctgca cggtatcgct tggtaatttc
     8881 attggacaac atggtaccga tggcgcggtc ggtgttgacn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnntggccg atcattgctt ccaccgtgcg gaagccgagc tgcgccatga
     9181 tttcgcggag ttcctgcgtg aggaatttga aaaaatggac gacgtggtca gggtcgccgc
     9241 tgaagcgctt gcgcaattca gggtcttgag tagccacgcc caccgggcag gtgttgaggt
     9301 ggcatttgcg catgaggatg cagccctcca ccaccaatgc agcagtggcc acgccccatt
     9361 cttcggcgcc cagcaaggcg gcgatggcta tgtcgcggcc ggttttgagc tgcccgtcgg
     9421 cctgcaccac taccctactg cgaagcttgt tgcgcaccag cgtttggtgg nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nngcccgcag caatggtgcc tacgcctgct ttcgacacga gtttcacgtt gatgcgggcc
     9661 tcgcggttgg cgtttttcaa atcaaaaatg agttgcgcca ggtcttcaat ggaataaata
     9721 tcgtggtggg gcggcggcga aatcagcccg acccccggcg tggagtgccg cactttaccg
     9781 atccactcgt ccactttgtg gcccggcaat tgcccgccct cgccgggttt ggccccctgt
     9841 gccattttga tttgcaactc ctcggcctcg ctgaggtaca aactcgtgac gccgaaaagc
     9901 cccgatgcca cctgcttgat gnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggcagcggtt
     9961 cgtagcggct ttcgtcttct ccgccctcgc cggtgttgct ttttccgccc aagcggttca
    10021 tggcaatggc cagtgtggtg tgcgcttccc aggaaatgga cccaaacgac atggcgcctg
    10081 tggcaaagcg gcgatagatg ttttcagccg gttccacttc ttctatcggt accggcgggc
    10141 gctgggagtt gaactcgaac aggctgcgga gcgtgacggc cttttccgac tgatcgtcaa
    10201 tgactttgga gtattttttg aaggtggcgt aatcgttctg ccgggtggcg tattgcaaca
    10261 aatgaatgct ctgcggattg aacaaatgca cctctccgcg ccgcttccat tgaaaaatgc
    10321 cgccttcggg caaacggctg ccctcctccg ttccggcccc gaacgcccac tggtgcttca
    10381 tcagcgtttc gcgggctatc tcgtccaagc ccatgccttc gatgcgcgaa acggccccgg
    10441 tgaagtactg gtccaccact ttccggttga tgccgatgat ctcgaaaatc tgagcgccca
    10501 tgtacgattg cagggtggaa atgcccattt tggaaaacac cttcaagagg ccttccttca
    10561 cggctttgat gtaattctgt tccaattttg ccgccgacaa ttcggggttg atgcgcccgt
    10621 cttggtgcaa atcgcggatg ccggccaaag ccatgtacgg gttgatggcc gtagccccga
    10681 agcccagcaa acaggcgaag tgatgcactt cccacacgtc gccggcttct accacgatgc
    10741 ccaccttgcc ccggtagccc ttgcggatga ggtggtggtg aatggccgag gtgaagagca
    10801 gcgagggaat ggccgcgtgt tcggagtcaa tggcccggtc ggagagcacg agcacttcga
    10861 agccgtcttc caccgcgtcc acggcatagc ggcacacgcg gtcgagggct tttttgagcg
    10921 aacccggttt gccgtcggcg cggaaataca tttgcagggt tttggcctga aaaatgccgg
    10981 tgtctatgct gcggattttt tccaattcgt ggttggtcat caccggatgc ggcagggcta
    11041 cggtgtggca cacttcgggg tcttcctcca aaatgttgcc gatattgccc ataaaagtgg
    11101 ccagcgacat gacggtgcgc tcgcggatgg ggtcaatggg cgggttggtg acctgcgcaa
    11161 agagttgtnn nnnnnnnnnn nnnnnnnnta atacgacaag tgctgcgggc ggtcgctgag
    11221 gatggccaac ggaatgtcgg tgcccatact gccgatgggt tctttgccgt ccaaggccat
    11281 cggcaggagg atgtcttcga ggtcttcgga agtatagccg aaggcctgct ggtatttgaa
    11341 cacctgcgcg ggttcgagcg gagcgaaggt gacgcgggga tcgggcagtt cctcaatgcg
    11401 gattttgtat ttttccagcc actcggcata aggccgggca gtgcaaattt cctgtttgat
    11461 ctcctcgtcg ccgatgacgc ggccctgcac caaatccacc agaaacatct tgccgggttg
    11521 cagtcggcct ttttctctca caatgtccgg cggcaggggc agcacgcccg tttcggaagc
    11581 caatatgacg cgctcgtcgt gggtgatgag gtagcgggcg gggcgcaggc cgttgcgatc
    11641 taaggtagcg ccgatgagtt ggccgttggt gaaacagatg gctgcggggc cgtcccaggg
    11701 ttccatgaag gaggcgtggt attcataaaa agcctttttg taggccgcca tgtgggggtt
    11761 gccgtcccag gcttcgggaa tgagcatcat catgacctgc ggcagggagc gcccgctgag
    11821 ggtgagcagc tcgatcatgt tgtctatgta cgccgagtcg gactgcccgc cggacaccac
    11881 cggcaacagc atttccattt cttcccggct gaacgacgcc gattgtaggc ttttttcgct
    11941 ggttttcagc cagttgagat tgccttgtat ggtgttgatt tcgccgttgt gggcgatgaa
    12001 ccggaagggc tgggccagct tccaagaggg gaaggtattg gtggcaaacc gcgaatgtac
    12061 caatccgaaa gcactcacaa agcgcgcgtc ctgtaggtcg gggtagtaat agcgcagttg
    12121 caggctggtg agctggcctt tgtacaccag cgtccggcac gacatggagg cgaagtacag
    12181 gcccaccgga tcatcggacg gaatgccggc ggcggccatg tgcgaagcgt gattgcgcag
    12241 cacatacagt ttgcgttcaa actcctccgc gttggaaatg gtatcggggc gggcgatgaa
    12301 cacctgctcg atttcaggct ctaccgagag cgccgtctcg ccgatgccgg cagggttgac
    12361 gggcactttg cgccaggcca gcacctccaa gcccagccgt tcggcactgc gcacaatgac
    12421 ttcccgacag gcttcgcggc ggcgcaggtc tttagggaaa aatgccatgc ccacgccgta
    12481 gcgctccggt tcgggcagtc gcacgccgag tcggaggcac tcatcgaaca aaaacgcgtg
    12541 tggaatctgt acggcaatgc ctgcgccgtc gccggtattt tcctcgcagc cacaggcgcc
    12601 ccggtgctcc atgttctcca ggatggtgag cgcgtccgag acggtttggt ggtttttgtt
    12661 gtttctcaga ttggcaataa aaccaatacc gcaggcgtcg cgctccatgt cggcgcagta
    12721 cagaccttgt ccatgttgtc ggctcatatt ttttcaaaaa aaattagtga gggccgtatt
    12781 tggcagggac aagcacaggc aagacatttg gcctcgctgt gtatttattt ggttttattg
    12841 aaaatccgac aaaaaatcca aataatcatt gatgattttg ccgggaaagg gcaaatttac
    12901 gataaaaaat gaaatatggg gcgttagggg tgaggtgcgg tatcttctac gtttgtcgct
    12961 ctccgcctcc tctccgtttg tcgctcttcc gtttgnnnnn nnnnnnnnnn nnnnnnnnnc
    13021 tccacttcgt tgcggattga caaaacgtag agcagcgtag aggggaccgt agagcgcgac
    13081 ccggcctcgc cggaaggcat atcgtagagc gagactgcgc tgaaatgtag cattttacag
    13141 gcgggtggaa cctgcgtaat actcacgccc ctccctgcgg ggtaaacatg aagatggaat
    13201 ctcggcgcga gcgggcaggc ggggggtacg ggta
//
LOCUS       DAORLF010000064        13093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2541_length_13093_cov_2.144654, whole genome shotgun sequence.
ACCESSION   DAORLF010000064 DAORLF010000000
VERSION     DAORLF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_13093_cov_2.144654"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1591
                     /locus_tag="PK198_07465"
     CDS             146..1591
                     /locus_tag="PK198_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRF38609.1"
                     /translation="MKSAILVFMLGCAVVLYSQNEVKPPAPVFPIPSAAQMAWHQMEM
                     NAFVHFTTNTFTGKEWGFGDENPGIFNPAQANVQQWINIFQKAGFRGVILTAKHHDGF
                     CLWPSTYTHHSVVNSPYKGGKGDIVMEVAEGCKRYGLKMGVYLSPWDRNHARYGTPEY
                     VDYYRNQLKELIADYGPFFEIWFDGANGGDGYYGGAKDTRKIDGRTYYDWPGTINMVN
                     KINPNVIFFSDAGPGCRWVGNEKGVAGETNWNTISVDTLYAGKPNIEQLLNTGSEDGT
                     SWVPAEVDVSIRPGWFYHQEEDSQVKTPEKLFEIYLSSVGRGAVLLLNVPPDRRGLIH
                     ENDIQSLKGFRRILDRTFKINLAQKASVRASSHRGNHPLFAPKNLLDDDFDSYWATED
                     NETTANVEITLKSERTLQYIELQEYIKLGQRVKSFSVEAWLNNQWQTVATGTTIGYKC
                     ILPLNSIKTAQLKITFRDSKACLAISKISIY"
     gene            1747..3504
                     /gene="aspS"
                     /locus_tag="PK198_07470"
     CDS             1747..3504
                     /gene="aspS"
                     /locus_tag="PK198_07470"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRF38610.1"
                     /translation="MSYRSHNCGELRLSDAGKTVKLSGWVQIGRDFGGLTFIDLRDRY
                     GITQLVFNMEDDAALCEQARKLGREFVVSVEGLVRERTSKNPNRATGDIEIEAKSLDI
                     LNPAKTPPFTIEDNSDGGDDLRMRYRYLDLRRSPVQHNIIFRSRLALETRKFLSDKNF
                     LEIETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQILMVAGYDRYFQIVRC
                     FRDEDLRADRQPEFTQIDCEMAFVTREDVLNTFEGLTKHLFRSMLGLELSNFPRMSYD
                     DALRLYGSDKPDLRFDMQFCELNDLAQGKGFSVFDDAELVVGICAKGIAESYTNKDLN
                     DLTDWVKRPQIGAKGLVYAKFNTDGSIKTTVGKFFSDDQIREWGQKMGAQTGDILFIL
                     SGEKEKTRKQLGELRLELGRRLGLMKPGDFKPLWVLDFPLLEWDEDSQRFFAMHHPFT
                     SPKTEDLARMMTDDFETLKSLRADAYDLVLNGGGSIRIHDRELQSKNFKLLGFTPEQA
                     EAQFGFLLGAFEFGAPPHGGIAFGFDRLCAVMNGQSSIRDFIAFPKNNQGRDMMIDAP
                     SPVADEQLDELCLRVELEE"
     gene            3865..4080
                     /locus_tag="PK198_07475"
     CDS             3865..4080
                     /locus_tag="PK198_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38611.1"
                     /translation="MVDIKKRQFSNLQLTLLELYDKEVSEDDLRNIHDLIGKYFAERL
                     TENATRVWDEQKWSEETMYNWLNDPNQ"
     gene            4322..5407
                     /gene="recA"
                     /locus_tag="PK198_07480"
     CDS             4322..5407
                     /gene="recA"
                     /locus_tag="PK198_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRF38612.1"
                     /translation="MEPINSKDREAKLKSLQMAVDRLEKTYGKGTVMRMGDSKVMDVE
                     VIPSGSLGVDIALGINGFPKGRVVEIYGPESSGKTTLAIHAIAECQKQGGIAAFIDAE
                     HAFDRHYAANLGVDTDNLLISQPDNGEQALEIAENLIRSGAIDIIVIDSVAALVPRSE
                     IEGEMGDSKVGLQARLMSQAMRKLTGSIGRTGCCCIFINQLREKIGVMFGNPETTTGG
                     NALKFYASIRVDIRKTGAGIKDKDGNIVGNNVKVRIVKNKLAPPFRIAMFDIMFGEGI
                     SKTGEIVDMGVDQEIIQKSGAWYSYNGAKIAQGREGAKQFMADNPEVAIDIEKKIKEK
                     LNSGAAATSPLKGIVAADDAEPGADDF"
     gene            5788..6210
                     /locus_tag="PK198_07485"
     CDS             5788..6210
                     /locus_tag="PK198_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38613.1"
                     /translation="MNLNNQLTLSILSGEALTLHFGDVLQPVKEHLARPALVPVNQWD
                     DDEEELEDDDDMDFDVEEEDADDEDGWEEEEFDIDEEDYDLEEDEEDMDFDSFGDDDE
                     EDGYYDDEPEDMLGHQVEDRNPSPCNPAYAVEARMELL"
     gene            6210..6956
                     /locus_tag="PK198_07490"
     CDS             6210..6956
                     /locus_tag="PK198_07490"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HRF38614.1"
                     /translation="MPAVTIDEVIRQLESIVEEASAAGDPLGIFAVVYLGVTRDVKSG
                     IDSGKFEDGARMARLDVIFANRYLDALALYRNSEPCTRSWEIAFEAAQNQDFLILQHL
                     LLGMNAHINLDLGIAAAESVEPNRISELERDFMSINRVLSEKIDEVQDRLSRVSPLLF
                     LLDWAGQRKDEQFAEFSLIQARNQAWQSALNLAKGDAGQQASAIQDLDALVAALGRLI
                     SHPGKWIGWLLKLIRLFEKKDVRQVMALLR"
     gene            complement(6961..8208)
                     /gene="ilvA"
                     /locus_tag="PK198_07495"
     CDS             complement(6961..8208)
                     /gene="ilvA"
                     /locus_tag="PK198_07495"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF006390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRF38615.1"
                     /translation="MNLQDTAHAIHEAARRLQQVVRRTPLMRHEGLSQRYNCSVWLKR
                     EDLQVVRSYKLRGAYNLMSTLSPEQIQRGVVCASAGNHAQGFAWSCRKLGLHGIVFMP
                     VITPRQKVSQTQMFGQDNIEVRLVGDTFDSCAAAAQEFAATQNKTFIPPFDHQRIIEG
                     QGTVALEILDDLPQIDYLFVPVGGGGLSAGMGSYFKTFSPATQIIGVEPLGAPAMYEA
                     LRAGQPITLETIDRFVDGAAVKRAGDLTFEHCQQVLDDLILAHEGLVCSTILKLYAED
                     AIVVEPAGALSIAALEQYEDQLAGKNVVCVVSGGNNDIDRMPEIKERALQYEGLKHYF
                     IIRFPQRPGALREFVVEVLGPDDDIVHFEYMRKNYREAGPALVGIEFKQTGQLDRLQE
                     SMRSHHFSFDYLNGNSDLLRFLL"
     gene            complement(8205..9275)
                     /gene="leuB"
                     /locus_tag="PK198_07500"
     CDS             complement(8205..9275)
                     /gene="leuB"
                     /locus_tag="PK198_07500"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRF38616.1"
                     /translation="MNKHIALLPGDGIGPEVTQQAVKVLDTIALRFGHQFTYTSADVG
                     AIAIERHGDPLPDATLETCLAADAILFGAIGHPRYDNDPTAKIRPEQGLLRLRKSLGL
                     FANIRPVSVYPTLAHLSPLKTEKVTGVDFVIFRELTGGIYFGERQRTASDAFDVCSYS
                     APEIERIARQAFHYARNRRGKLTLVDKANVLDTSRLWRSVVTEMAASEFPDVRFDWMY
                     VDNAAMQIIQFPADFDVILTENMFGDILSDEASVLPGSLGLLPSASTGEHTALFEPIH
                     GSYPQAAGQDIANPVAAILSAAMLLDHFSFHTEADTVRQAVRSVLEAGIGTPDLKAEK
                     TVGCKEMGQKVADLLAPGNALT"
     gene            complement(9315..9914)
                     /gene="leuD"
                     /locus_tag="PK198_07505"
     CDS             complement(9315..9914)
                     /gene="leuD"
                     /locus_tag="PK198_07505"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRF38617.1"
                     /translation="MQKFTRLTSSAVPLPIEDVDTDQIIPARFLKATTREGFGENLFR
                     DWRFHPDGSPKPDFVLNNSIYQGAILVAGRNFGCGSSREHAAWALYDYGFRVVVSSFF
                     ADIFRGNALNNGLLPLQVSPEFLHLIFSAIEADPQTGFSVDLEQQTIRIGADGPQAHF
                     DIDPFRKVCMLNGYDDIDYLLSIKNDIEQFEQQLVARGS"
     gene            complement(10093..11487)
                     /gene="leuC"
                     /locus_tag="PK198_07510"
     CDS             complement(10093..11487)
                     /gene="leuC"
                     /locus_tag="PK198_07510"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRF38618.1"
                     /translation="MAGLTLFDKIWNAHVVKSVEGGQDVLYIDRHLIHEVTSPQAFDG
                     LRKRGIPVFRPAQTIATADHNVPTLNQHLPISEPLSRFQVDKLTENCEAFGVELYGLG
                     HQRQGIVHVIGPELGITQPGMTLVCGDSHTSTHGAFGSIAFGIGTSQVEQVLATQCLL
                     QKRPKRMRITVDGTLSEGVTSKDIILHIIARLSAAGATGFFVEYAGSAIRSLSMEGRM
                     TICNMSIEMGARGGLIAPDETTFEYVRGREFAPQGEAWEKSLKYWQTLFTDADAAFDE
                     EHHFNAADIAPILTFGTNPGMGIPVDGSIPQAPSESSASFDKSLEYMGFQAGESLLGK
                     PIDYVFIGSCTNSRIEDLRLAARIVQGKKVPAHVTAWVVPGSKQVEQQAKAEGLDRIF
                     TAAGFEFREPGCSACLGMNEDKIPTGKYCISTSNRNFEGRQGPGARTILAGPAMAAAA
                     AVEGKIIDVRSLST"
     assembly_gap    11664..11862
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11863..>12241)
                     /locus_tag="PK198_07515"
     CDS             complement(<11863..>12241)
                     /locus_tag="PK198_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRF38619.1"
                     /translation="GRADNEFLARVLEAAIQAGATVLNIPDTTGYCLPEQYGEKIRYL
                     AENVRGIEKAILSTHCHNDLGLATANSIYGVINGARQIECTINGTGERAGNTSLEEVV
                     MILRQHAELGFSTGINTRLLNPVS"
     assembly_gap    12242..12271
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12272..12681)
                     /locus_tag="PK198_07520"
     CDS             complement(<12272..12681)
                     /locus_tag="PK198_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRF38620.1"
                     /translation="MAIPVHIFDTTLRDGEQVPGCKLNTAQKIEIAHALEALGVDIIE
                     AGFPISSPGDFESVQQVARTVKHATVCGLSRAVEKDIKVAAESLVGAKRPRIHTGIGV
                     SEMHIRYKLRTTPEDIIHRAAAAVKYAKSFVEDV"
     gene            complement(12878..>13093)
                     /locus_tag="PK198_07525"
     CDS             complement(12878..>13093)
                     /locus_tag="PK198_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38621.1"
                     /translation="HLLKISCIICMLTFYYTLYLYGSGDWGLLAIMQKVSLISSILLI
                     LGLEYFTQKEDFKQGDQNRQNARPLQQ"
BASE COUNT         3003 a         3197 c         3627 g         3037 t
ORIGIN      
        1 caacacccga aagtcaggca tagttgactg atttattgat aatcatttac tgcaccttct
       61 gatcttatgc tgaatatgcc ccggcaatta taactgatgc tgtttgactt tcactatctt
      121 tgtagtgagg caaaaaaaga aatatatgaa atcagctatt cttgtattca tgctcggctg
      181 tgctgtcgtg ttatattccc agaacgaggt caaaccgccc gcaccagtgt ttccgattcc
      241 atcggcggcg caaatggcct ggcatcaaat ggaaatgaat gcctttgtgc attttacaac
      301 caataccttt accggaaaag aatggggctt tggcgatgaa aaccctggta ttttcaatcc
      361 tgcccaagcc aatgtgcaac aatggatcaa catctttcaa aaagccggtt tcaggggcgt
      421 tattctcact gcaaagcatc acgacggttt ttgcttgtgg ccttctacat acacacatca
      481 ttctgtcgtc aacagtccat acaaaggcgg taaaggagat attgtaatgg aggtagcaga
      541 aggatgtaaa agatatggtc tgaaaatggg agtatattta tcgccctggg acagaaatca
      601 cgcccggtat ggaactccgg agtatgtgga ttattacaga aatcaactga aagaactgat
      661 tgctgattat ggccctttct ttgaaatatg gtttgacggc gcaaatggcg gagacgggta
      721 ttatggaggt gcaaaggaca caagaaaaat tgatgggcga acttattacg attggccggg
      781 aaccatcaat atggttaata aaataaaccc caatgtgata tttttcagcg atgccggccc
      841 gggctgtcgt tgggttggca acgaaaaagg ggttgccggt gaaacaaact ggaacaccat
      901 ttctgtggat acgctgtatg ccggaaaacc caatattgaa caactgctca acacgggatc
      961 agaagatggt acaagttggg tgcctgctga ggtggatgta tctattcgac cggggtggtt
     1021 ttatcatcaa gaagaagatt cgcaggtgaa aacgccggaa aaattgtttg aaatctacct
     1081 ttcgtcggtg ggaagagggg cggttctttt acttaatgtt cctcccgacc gccggggctt
     1141 gattcacgaa aatgatattc aatcgctaaa gggcttccgc aggattttag accgcacatt
     1201 taaaatcaat cttgcccaaa aggcatcagt gcgggcaagc agccatcggg gaaatcatcc
     1261 attatttgcg ccgaaaaacc tgctggacga cgactttgat tcctattggg ccactgagga
     1321 taacgaaacc actgcaaatg ttgaaattac cctgaaatct gaaaggaccc tccaatacat
     1381 cgagttgcag gaatacataa aacttggcca gcgtgtgaaa tcattttcgg ttgaggcttg
     1441 gcttaataat caatggcaaa cggtagcaac aggcacaacg attggataca agtgcatttt
     1501 accattgaat tccataaaaa ctgctcaatt gaaaatcact tttagggatt caaaggcatg
     1561 ccttgccatt tcgaagattt caatttactg agcaatggcg caatcgccgt tcccacctgc
     1621 agcttgcagc ctgtagctca cggcttgcag ctattgacaa aacctaaaat atataaaaca
     1681 aaaaccccct cattccttac ctttgcgccg ctattcaaaa aatcactttt gcaaaagtaa
     1741 cacaccatgt cataccgttc tcacaattgc ggcgaactgc gcctcagcga tgcaggaaaa
     1801 acagtaaaac tcagcggttg ggttcaaatc gggcgcgatt tcggcggcct cacttttatt
     1861 gacctccgcg accgctacgg cattactcag ttggttttca atatggaaga cgatgcggcc
     1921 ctctgcgagc aggcccgcaa actcggacgc gaattcgtcg tctccgtcga ggggcttgtg
     1981 cgcgaacgca cgagcaaaaa ccccaatcgc gctaccggcg acattgagat cgaagccaaa
     2041 tcacttgaca tcctcaatcc ggccaaaacg cctcccttca ccatcgaaga caacagcgac
     2101 ggcggcgacg acctccgcat gagatatcgc tacctcgacc tccgccgcag ccccgtacag
     2161 cacaacatca tcttccgcag ccgtctggcg ctggaaacgc gcaagttcct ctccgacaag
     2221 aacttcctcg aaatcgaaac gcccttcctc atcaaaagta cgcccgaagg cgcccgcgat
     2281 ttcgtcgtgc cttcccgcat gaatccgggc cagttttacg ccctgccgca gtcgccgcag
     2341 acgttcaaac aaatcctcat ggtggccggc tacgaccgct attttcagat tgtgcgctgc
     2401 ttccgcgacg aagacctccg cgccgaccga cagccggaat tcacgcaaat agactgcgag
     2461 atggccttcg ttacccgcga agacgtgctc aacaccttcg agggcctcac caaacacctc
     2521 ttccgcagca tgttgggcct cgaactaagc aatttccccc gcatgtctta cgacgacgcc
     2581 ctgcgcctct acggctccga caagcccgac ctccgcttcg atatgcagtt ttgtgaactc
     2641 aacgacctcg ctcaaggcaa aggcttctcc gttttcgatg acgccgaact cgtggtcggc
     2701 atctgcgcca aaggcatcgc cgaaagctac accaacaaag acctcaatga cctcaccgac
     2761 tgggtaaaac gcccccaaat cggcgccaag ggcttggtgt atgccaaatt caacaccgac
     2821 ggcagcatca aaaccacggt gggcaagttc ttcagcgacg accaaatccg cgagtggggc
     2881 caaaaaatgg gcgcccaaac cggtgatatt ctcttcattc tcagcggtga aaaggaaaag
     2941 acacgcaaac aactaggcga actccgcctc gaactgggcc gtcgcctcgg cctcatgaaa
     3001 cccggcgatt tcaaaccgct ctgggtactc gatttccctc ttctcgaatg ggacgaagat
     3061 tcccagcgct tcttcgccat gcaccacccc ttcacctcgc ccaaaaccga agacctcgcc
     3121 cgcatgatga ccgacgactt cgaaacgctc aaaagcctcc gcgccgatgc ttacgacctc
     3181 gtcctcaacg gcggcggttc catccgtatc cacgaccgcg aactacagtc caaaaacttc
     3241 aaactgctcg gcttcacgcc cgaacaggcc gaggcgcagt tcggcttcct cctcggcgct
     3301 ttcgagttcg gcgcgcctcc gcacggcggc attgccttcg gcttcgaccg cctctgcgcc
     3361 gtcatgaacg gccaaagctc catccgcgac ttcatcgcat tccccaaaaa caaccagggc
     3421 cgcgacatga tgatagacgc gccctcgccc gtcgccgacg agcaattgga cgagctttgc
     3481 ctgcgcgtgg agctggagga atgagaaggt agtttttcgt gagtttggtt ttgggcgtgc
     3541 cggccccgct gcttatctcc tgaaaacaga taaactggtt gggccgtcgg gtgcttcggt
     3601 cgctcccaat aggcgatgca aaaataaagg ttcattttta ccctgaaaaa tgaacctttt
     3661 gcccactacc aaggtcacta ccctcagccc cctcacgcgt gtcgggcgag agctgttagc
     3721 tttcatagca acagccggtt aaagctaaca gctcttcaaa ccaaagccgg catctctgca
     3781 ccgacagggt ttgtgttata atgtacaaaa cactcaactt ttcaaagaat aaagtagttt
     3841 tgtctgaaaa tactcttcat taagatggtt gatattaaga aacggcagtt ttccaatctc
     3901 caacttacac tgcttgagtt gtacgataag gaagtaagcg aagacgacct tcgcaatatc
     3961 cacgacctta ttggtaaata ttttgctgaa cgcctgactg aaaatgctac ccgggtttgg
     4021 gatgagcaaa agtggtctga ggaaactatg tataattggc tgaatgaccc caatcaatga
     4081 gaattgtatt agacaccaat gtattgttag ttgcacttcc tgctcagtcc ccgttctttc
     4141 ctatctttaa ggcatttctc gattattatc aaggccgaag cgttcctgaa cgtgctacct
     4201 tcgctttatc acgatcacag ttagtcgccc gcctcccgaa atttttttca aaaaaagttt
     4261 gcataaataa acaaaacatt tatttttgca tcatcaaaaa acaaaaaagt taatcaagct
     4321 catggagcca atcaacagca aggaccggga ggctaaactc aagtccctcc aaatggcggt
     4381 agaccgcctc gaaaaaactt atggcaaagg taccgtcatg cgtatgggcg actccaaagt
     4441 catggatgtg gaagtcattc cctccggctc actcggtgtg gacattgcct tgggtatcaa
     4501 tggtttcccc aaaggtcgcg tcgtagaaat ctacggcccg gaatcctccg gtaaaaccac
     4561 gctcgccatt catgccattg ccgaatgcca aaaacagggc ggcatcgctg cgttcattga
     4621 cgcagagcac gccttcgaca ggcactacgc cgccaacctt ggcgtggata ccgacaacct
     4681 gctcatctcc cagcccgaca acggcgagca ggcactcgaa atcgccgaaa acctcatccg
     4741 ttccggcgcc attgacatca ttgttataga ctctgtggcc gccctcgtgc cccgcagtga
     4801 aattgaaggc gaaatgggcg actccaaagt gggccttcag gcgcgtctca tgtcgcaggc
     4861 catgcgcaaa ctcacgggtt ccattggccg cacgggctgc tgctgcatct tcatcaacca
     4921 gttgcgcgaa aaaatcgggg tcatgttcgg caatcccgaa accaccaccg gcggtaatgc
     4981 cctcaagttt tatgcctcta tccgcgtgga cattcgcaaa accggcgcag gtatcaaaga
     5041 taaagacggc aacatcgtcg gcaacaacgt caaagtgcgt atagtaaaaa ataagcttgc
     5101 gccgcctttc cgcattgcca tgttcgatat catgttcggt gagggtatct ccaaaaccgg
     5161 cgaaattgtg gacatgggag tagatcagga gattatccag aaaagcggtg cctggtatag
     5221 ctacaacgga gctaaaattg cccagggccg cgaaggcgcc aaacagttca tggccgacaa
     5281 cccggaggtg gcaattgaca ttgagaaaaa aatcaaggag aagttgaact ccggagctgc
     5341 tgccacgagt ccgctcaaag ggatcgtcgc tgccgatgac gccgagccgg gcgctgatga
     5401 tttttaaagt caatttttca accacatagt cacagatgga tggccccata ttggagtcca
     5461 tgtaccctat cgtgactatg tggttgtatc tctgtcgaaa attaaaaccc agaagaattt
     5521 cccaagtggt gacctcacca agtctgtcgg tatgagtgac gccgcccagt tgagtaccct
     5581 ttgttcgggg ggctatagta tggaactgaa ccccggatgc acttgtcggt atcgttagaa
     5641 actacagggg ggcggcacgc cctgaaaaaa agtaaaaaaa attcggtgag gggcatacga
     5701 gcaatttagg cataatgttt actttagccc tgcgattgaa aggtcgcgtt gcccattcac
     5761 atttttgttt taccagatca actgattatg aacctgaaca atcaactcac cttatccatt
     5821 ttgagcggcg aagctctgac gctgcatttc ggagatgtgc ttcagcccgt aaaggaacat
     5881 ttggcacggc ctgccttagt gcctgtgaat caatgggatg atgatgaaga ggaattggaa
     5941 gatgacgatg atatggactt tgatgtagag gaggaggatg cggatgatga ggatggttgg
     6001 gaagaagagg agtttgatat agatgaggag gattatgacc tcgaagaaga tgaggaggac
     6061 atggattttg attcttttgg agacgacgat gaggaggatg gctactacga cgatgaaccg
     6121 gaggatatgc tgggacatca ggtagaagac cgcaatccgt cgccgtgcaa cccggcgtat
     6181 gctgtggagg cgcgcatgga gttgctctga tgccggccgt tacgattgac gaggtcatac
     6241 ggcagttgga atccatcgtg gaagaggcat ctgctgcggg ggatccgctg ggaattttcg
     6301 cagtggtgta tctgggagtt acgcgcgatg tgaaatcggg tattgactct ggaaaatttg
     6361 aggatggcgc ccgcatggcg cgcttggatg tcatatttgc caacagatac cttgatgcgc
     6421 tggccttgta tagaaattcg gaaccctgca cccggtcgtg ggaaattgca tttgaagctg
     6481 ctcagaatca ggactttctg atactccagc atttactgct gggaatgaat gcgcatatca
     6541 atttagactt gggaattgct gcggctgagt cggtggaacc gaaccgaatc tcggaattgg
     6601 aacgcgattt tatgtctatc aaccgggtat tatcggagaa aattgatgag gtgcaagacc
     6661 gtctgagcag ggtttcgccc ttgttgttcc tgctggattg ggctgggcag cgaaaagatg
     6721 agcaatttgc ggagttcagt ttgatacaag ccagaaatca ggcatggcaa tcggctttga
     6781 atctggcgaa aggggacgcc ggacagcaag cttctgcgat tcaggatttg gatgctcttg
     6841 tagctgcttt gggaagactt atctcgcatc ccggcaagtg gataggctgg ttgttgaagt
     6901 tgattcgatt gttcgagaaa aaagacgttc ggcaggtgat ggccttgctt cggtgaatac
     6961 ttacaacaaa aacctcaaca aatcactgtt tccattgaga taatcgaagc tgaaatgatg
     7021 gcttctcatg ctttcctgta agcggtcgag ctggccggtt tgtttaaatt ctatgcccac
     7081 caatgcaggc ccggcttcgc ggtagttttt gcgcatgtac tcgaagtgta cgatgtcgtc
     7141 gtcggggcca agtacctcca ccacgaattc gcgcagagcg ccggggcgct gagggaatct
     7201 gatgatgaag tagtgcttga ggccctcgta ttgcagtgcg cgctctttga tttcgggcat
     7261 gcggtctatg tcgttgttgc cgccggagac aacgcaaaca acattttttc cggccaattg
     7321 atcttcatat tgctccaacg cagcaatact gagcgcgccg gcaggttcta ccacgatggc
     7381 atcttctgcg tagagcttga ggatggtgct gcacaccaat ccttcgtgcg ccaaaatcag
     7441 gtcgtccaac acctgctggc aatgctcgaa ggtgagatcg ccggcgcgtt tgacggcggc
     7501 gccgtccaca aaccggtcaa ttgtttcgag cgtgatgggt tggccggcgc gtaaggcttc
     7561 gtacatggcc ggtgcgccaa gtggctctac tccaatgatt tgtgtggcgg gagaaaaggt
     7621 tttaaaatag gaacccatac ccgcgctgag tcctccgcca cccaccggca cgaagaggta
     7681 atctatttgc gggaggtcgt ccaggatttc caaagccacg gtgccttgcc cttcgatgat
     7741 gcgttggtgg tcgaaaggag ggatgaatgt tttgttttgc gtggccgcaa actcttgagc
     7801 ggcggcggcg caactgtcga aggtgtcgcc cacgagtcgc acttcgatgt tgtcctgccc
     7861 gaacatctgg gtttgagaca ctttctgccg gggtgtgatg acgggcatga acacgatgcc
     7921 gtgcaggccg agtttgcggc aagaccatgc aaagccttgc gcgtgattgc ccgcgctggc
     7981 gcataccacg ccgcgctgta tttgctcggg gctgagggta ctcatgaggt tgtaagcgcc
     8041 ccgaagtttg taagagcgca ccacctgaag gtcttcgcgc ttgagccaaa ctgagcagtt
     8101 gtagcgctgt gagaggccct cgtggcgcat gagcggggtg cggcgcacta cctgctggag
     8161 gcgtcgggcg gcttcgtgga tggcgtgtgc ggtgtcttgc aaattcatgt taaagcattg
     8221 cctggtgcca ataaatccgc tactttctgt cccatttcct tgcagcccac tgttttttct
     8281 gcttttagat cgggggtgcc gatgccggct tcgaggacgg agcggacggc ttggcgcacg
     8341 gtatcggctt cggtatggaa gctgaaatga tcgagcagca tggcggctga gagtatggcg
     8401 gctaccgggt tggcaatgtc ctgaccggcg gcctgcgggt aagaaccatg tatcggctcg
     8461 aaaagtgcgg tatgttcgcc agtggacgcg gagggcagca gtccgagcga accgggcaac
     8521 acgctggcct cgtcggagag gatgtcgcca aacatatttt cggtgaggat gacgtcgaaa
     8581 tcggcgggaa actgaatgat ttgcatggcg gcattgtcca catacatcca atcgaaccgg
     8641 acatcgggaa attcggaggc ggccatttcg gtgacgacgg aacgccacag acggctggta
     8701 tccaatacat tggctttgtc cacgagggtg agtttgccac ggcgattgcg tgcatagtga
     8761 aatgcctgcc gggcaatgcg ttcgatttcg ggggcggaat aggagcacac gtcgaaggcg
     8821 tcggaggcgg tgcgttggcg ctcgccgaaa tagatgccgc cggtgagttc tcggaaaatg
     8881 acgaagtcca cgccggtcac tttttcggtt ttgagcggcg agaggtgggc cagcgtggga
     8941 tagaccgaga ccgggcggat attggcaaac aaaccgaggc ttttgcgaag gcggagcagc
     9001 ccttgttcgg ggcggatttt ggccgttggg tcgttgtcgt agcgcgggtg gccgatggcg
     9061 ccgaaaagga tggcgtcggc ggcgaggcag gtttcaaggg ttgcgtccgg cagcgggtcg
     9121 ccgtgtcgtt cgatggcaat ggcgcccaca tcggcggagg tgtaggtgaa ttggtggccg
     9181 aaacgcaaag ctatggtatc cagtactttg acggcttgtt gagtgacttc cgggccgatg
     9241 ccgtcgccgg gcaggagggc gatgtgtttg ttcattggtt ttgcaatcat ttatttaatt
     9301 tcaaaagctg cgagctacga gccgcgagct acgagttgtt gttcaaattg ttcgatgtcg
     9361 tttttgatgc tgaggaggta atcaatgtcg tcgtagccgt tgagcataca gactttgcgg
     9421 aaggggtcaa tatcgaaatg tgcctgcggg ccgtcggcgc cgatgcgaat ggtttgctgt
     9481 tcgagatcaa cggagaaccc ggtttgggga tcggcctcta tggcgctgaa aatgaggtgt
     9541 aaaaactccg gcgagacttg caggggcagc agtccgttgt tgagggcatt gccccggaaa
     9601 atatcggcga agaaactgga cacgaccacc ctgaaaccgt agtcgtacaa ggcccaggcg
     9661 gcgtgttctc ggctgctgcc gcaaccgaaa ttgcgccctg ccacgaggat ggcgccctgg
     9721 taaatggaat tattgagtac gaaatctggt ttggggctgc cgtcggggtg gaagcgccaa
     9781 tcgcggaaga ggttttcgcc gaatccttcc cgcgtggtgg ccttgaggaa tcgggcgggg
     9841 atgatctggt cggtgtccac gtcttcgatg ggaagcggga cggcggagga ggtgaggcgg
     9901 gtgaattttt gcatggaaaa acaattgatt tttggaaagt actcgcttgt gcgcaaggga
     9961 taggagcggt accggcggcg gttggcatag aggtgtgccg ccggtaagag cggatagccc
    10021 ggccccgccc gcctgccttt gcagagcgaa tgtggggagg cggtttgagg cggggtgcgc
    10081 cccgaaaatg atttatgttg aaagcgatct tacgtcaata atttttcctt ccactgccgc
    10141 tgccgccgcc atagccgggc cggccagaat ggtacgggcg ccggggcctt gccgcccttc
    10201 aaaattgcgg ttggaagtgc tgatgcaata cttgccggtg ggtatcttgt cttcgttcat
    10261 gccgaggcag gcggagcagc ccggctcgcg gaactcgaaa cccgccgctg taaaaatgcg
    10321 atccaagccc tctgctttgg cctgttgctc cacttgtttg gaacccggca ctacccaggc
    10381 cgtgacgtgg gcgggcactt ttttgccctg tacgatgcgg gccgccagcc ggaggtcttc
    10441 gatgcgggaa ttggtgcagg aaccgataaa aacgtagtca atgggtttgc ctaaaaggga
    10501 ttcgcctgct tggaagccca tgtattccag cgacttatcg aaagaagcgc tgctttcgga
    10561 aggagcctgt gggatactgc cgtccacggg gatgcccatg ccgggattgg tgccgaaagt
    10621 gagtatgggg gctatgtcgg cggcgttgaa gtggtgctct tcgtcgaagg cggcatcggc
    10681 gtcggtgaaa agtgtttgcc aatacttcag cgatttttcc catgcctcgc cctgtggcgc
    10741 aaactccctg ccgcgcacat attcgaaggt cgtttcatcg ggggcgatga ggccgccgcg
    10801 cgcgcccatt tcgatgctca tgttgcagat ggtcatgcgg ccctccatgc tcaggctgcg
    10861 gatggcactg ccggcgtatt ctacaaaaaa accggtggct ccggcggcgc tcaggcgggc
    10921 gatgatgtgc aggatgatgt ctttggatgt gacgccttcc gagagcgttc cgtccacggt
    10981 gatgcgcatg cgtttgggcc gtttttgcag caaacactgg gtggccagca cttgctctac
    11041 ctggctggtg ccgatgccga acgcaatgct gccgaatgct ccgtgggtag aagtgtggct
    11101 gtcgccgcac acgagggtca tgccgggctg ggtgatgccg agttcggggc cgatgacgtg
    11161 tacgatgccc tgccgctgat ggccgaggcc gtagagttcg acgccgaaag cctcgcaatt
    11221 ttcggtgagt ttgtccacct ggaagcgcga caaaggctcg ctgatgggca ggtgttggtt
    11281 cagggtgggc acgttgtgat cggcggtggc gatggtttgc gcgggtcgga acaccgggat
    11341 accgcgtttt ctcagcccgt cgaaggcctg cgggctggtg acttcgtgga tgaggtggcg
    11401 atctatgtag agcacatcct gtccgccctc gacggacttg acgacatgtg cgttccagat
    11461 tttgtcaaaa agcgtgagtc cggccattat gctgctgcga ttttatctga gtgttgggaa
    11521 tttctgagtt cggaaacgat gaactggagg tcgtcgtcct gtacttcctt tttgcggtcg
    11581 gccacttcga ggaaggaggc gtaaatttcg tccaattcgt ctttagtgag atcgaagccg
    11641 aggcatttgt agcggtatgc gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggctcacg ggattgagca
    11881 gacgggtatt gatgccggtg ctgaagccga gttcggcgtg ctggcgcagt atcatcacca
    11941 cttcttcgag cgaggtattt cctgcgcgtt cgccggtgcc gttgatggta cattctattt
    12001 gtctggcccc gttgatgacg ccgtagatgg aattggcggt ggccagcccc aggtcgttgt
    12061 ggcagtgggt gctgaggatg gctttttcta taccgcgcac attttcagcg agatatcgga
    12121 ttttttcgcc gtattgttcg ggcaggcagt agccggtggt gtcgggaata ttgagcacgg
    12181 tggcgccggc ctgaatggca gcttccaata cgcgggcgag gaattcattg tcggcacgac
    12241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccacgtcc tccacaaagg atttggcgta
    12301 cttcacggcg gcggcggcgc gatgaatgat gtcttcgggc gtggtgcgga gtttgtagcg
    12361 gatgtgcatt tccgacacgc ctatgccggt gtggatgcgg gggcgtttgg ctccgacgag
    12421 cgactcggcg gctactttga tgtctttttc aacggcccgg ctgaggccgc aaacggtggc
    12481 gtgttttacg gtgcgggcca cttgctgcac cgactcaaaa tcgccggggc tggaaatggg
    12541 aaaacctgcc tcgatgatgt ccacgccgag ggcttcgagg gcgtgcgcga tttcaatttt
    12601 ttgcgcggtg ttgagtttac aaccgggcac ctgctctccg tcgcggaggg tagtgtcgaa
    12661 aatgtgaacg ggtatggcca tgatcttgct gttgtttgtc tcaggatgag tggccaaagg
    12721 taagcgagcg ccaggctgcc gggcaaacca taattgtcgg ctactgatat cgtctttttt
    12781 ggggtgtaac gaactggttt acagtttttt attgaaggtt tttatacgat gcccgaccat
    12841 tgtaaaggga aagaaacaaa ggtttgtttt ttgcttttta ttgctgcaag ggccgggcat
    12901 tttgtcgatt ttggtcacct tgtttaaagt cctctttttg ggtgaaatac tcaagtccta
    12961 aaattaataa aattgaagag attaacgata ctttctgcat aatagccaat aagccccaat
    13021 cgccggaacc gtataaatat aatgtatagt agaatgtcaa catacaaatg atgcaggaga
    13081 tttttaataa atg
//
LOCUS       DAORLF010000065        13074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2545_length_13074_cov_2.189569, whole genome shotgun sequence.
ACCESSION   DAORLF010000065 DAORLF010000000
VERSION     DAORLF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_13074_cov_2.189569"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK198_07530"
     CDS             <1..542
                     /locus_tag="PK198_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38622.1"
                     /translation="IAGIELPDNCIPGAAPARDTILLDGLSGTHFLFEVNLQNTISNE
                     GTLRITNEAYTLDLGNEKGLVLPFKQLRRIPDYTIWGYAGHSSPSAAGIAGQFVNELF
                     ARSTAANLQAGNYGYFSVAPGSGSITAANSPADLTLRPFAFRYTGNLDDLRSLTESYR
                     NQYGALLQIKLVAVTGEVF"
     gene            complement(548..>3055)
                     /locus_tag="PK198_07535"
     CDS             complement(548..>3055)
                     /locus_tag="PK198_07535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF38623.1"
                     /translation="GWHAMPYLDMGEFYSEFGSFEVEITVPENYVVAATGVLATETEQ
                     AFLKEKTKETETLLADWPERRQEAPFPASSERAKTIRFTADSVHDFAWFADKRFMVQH
                     RNLALPSGKTADAWAFFTPFEAHLWKRGAEYVAEAVQFYSTALGEYPWPQATAVEGAL
                     SAGGGMEYPMITIIGAAGNALSLDEVIAHEVGHNWLYGILASNERDDAWLDEGLNSYY
                     EHRYMKQRYGEGSSDVGIWPALKKASGMRTNEAAYLLQARRRMDQAPATTSNEFGGLN
                     YFIGAYNKPAWALQLLERYIGQEKMDAAMQQYYQIWQFRHPQPKDFRNVLEQTSGLDL
                     HWLFDGLIGSNRPLDYRISKGRKTGGEWRLSIQNKGRIAAPLHIAAMLGDSIVAGQWV
                     EGFEGVKELTFPAGDYDRFSIDPLRLSPDMNRRNNHLRSSGIFRRVEPLRLRLLPWLE
                     DDRHTVLHIAPALAANEYDGNMFGLALYNSTLPQRPFEFAAAPMYATRTGTLAGTGSV
                     QYHLYPAGKLLHGIHFGVGGRMFHFEYNERHDYHLQYARLTPFVKLELHRPAASSLSQ
                     TLQWRGIRQWAQSAEFAPDGTFSGLQRQAAWIQEWSYAGEQRRVVNPQSWRVAIERQK
                     DEDQFIPLNYWKASAEWKGAFTYEKGRHLHLRVFAGGFLSNSRRTRGAIFPEAFNLTA
                     QGFNDYRFDDYYFGRTEREGIWSQQISLREGGMKALTGAGFAAGRSNNFIIAMNLAAD
                     LPRQFLLPLKPYFDIGYFDNAMPTGQNDRLVDQILWSGGLALELGEGVFGIYLPLINS
                     KNLSARLAERGNFLQRIAFTFDLNRLNPWQLPDLFVY"
     assembly_gap    3056..3065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3066..3675)
                     /locus_tag="PK198_07540"
     CDS             complement(<3066..3675)
                     /locus_tag="PK198_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38624.1"
                     /translation="MIAYHKTAFLGSARTWFAALLAFCAGSTLAQPAYFQQETHYRIA
                     ARLDDRQHSLQCNMTLQYVNHSPDTLREIWWHLWPNAYSMRSTAFAKQALRLRKADLH
                     FAPDSSLGGISGLDFRADGRPALLNPDAKNPDLARLELPAPLPPGGSVSIETPFFLKI
                     PASFSRLGHVGQSYQLTQWYPKPAVYDRDGWHAARRGASGPSP"
     gene            complement(3672..4115)
                     /gene="folB"
                     /locus_tag="PK198_07545"
     CDS             complement(3672..4115)
                     /gene="folB"
                     /locus_tag="PK198_07545"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRF38625.1"
                     /translation="MAIIALEGIRLHGRHGYYPEEEILGTEFIIDVYVETNIKEAADS
                     DELMHTVNYETIYLICQREMKNTSSLIEKTAQNIADEINDFFPQVGGVKVRLRKMHPP
                     LGGSVHSAFVEIETGSFGWPKLRTLKKLREMLKNWSDLVKKFDQL"
     gene            complement(4145..6070)
                     /gene="dnaK"
                     /locus_tag="PK198_07550"
     CDS             complement(4145..6070)
                     /gene="dnaK"
                     /locus_tag="PK198_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRF38626.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNDEGRRTTPSVVAFMD
                     NGERRVGDPAKRQAITNPQRTVFSIKRFMGTRYSERVGEVSHVPYKVAKGDNDTVRVD
                     IDGRHYTPQEVSAMVLQKMKKVAEDYLGQPVTEAVVTVPAYFNDSQRQATKEAGEIAG
                     LTIKRIINEPTAAALAYGMDKRHKDMTIAVYDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHIIIDWLADEFKSQENMDLRKDPIALQRLKDAAEKAKIELSASSETEIN
                     LPYITAVDGVPKHLVIKLSRAKFEQLVDHLVERTLKPCEAALRDAGISKEQVDEIILV
                     GGSTRIPRIQQAVENFFGKKPNKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETLGGVYDVVIESNTTIPAKKSKVYSTAADNQPSVEIHILQGERQMARDNRTVGR
                     FILDGIPPAPRGLPQVEVSFDIDANGILSVTAKDKGTGKEQNIRIEASTGLSKDEVER
                     MKAEAAANADTDRQARERAEKINMADTLVFQTEKQLREYGDKIPANKKEAIESAAAEL
                     KEAHKMQDLDRIDSASKTLESAWQAASQEMYQATQEQQQQQQDYASDNGAYQQGGSNG
                     AAEDVTDVEFEEVKDNK"
     gene            complement(6352..6849)
                     /locus_tag="PK198_07555"
     CDS             complement(6352..6849)
                     /locus_tag="PK198_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38627.1"
                     /translation="MTNNNLFLICPFSSVEPFIRSKYGNENYFLTAPAGYFKFEEEPY
                     LQEVRNFIARNNPDNIYIVHDTSCRFLRAVVSEQEEQPEVTVCKPILEVFQRNRHAIM
                     EAPDTEAKVEKLAEYSMVQQTTHWVQSQVLGREIKKHGIQIKGLITNRQEQACKEVEI
                     NLMMD"
     gene            complement(6881..7111)
                     /locus_tag="PK198_07560"
     CDS             complement(6881..7111)
                     /locus_tag="PK198_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38628.1"
                     /translation="MKAKAILCSIACIACLALAFSSGSLIESRLFISLSVISGLLFFR
                     FASMPAPVARKREEKRQSAPAYKVQEAHIEHI"
     gene            complement(7141..>7959)
                     /locus_tag="PK198_07565"
     CDS             complement(7141..>7959)
                     /locus_tag="PK198_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF38629.1"
                     /translation="GAAMLADTPEVFDVVVTANLYGDVLSDVAAQLTGSVGLGGSANI
                     GEECSMFEAIHGSAPKIAGQNIANPSGLLQAAVMMLHHIGQTEVAETIQNAWLKTIED
                     GVHTADVFKEGVSKVKVGTRGFAQAVISNLGQKPAALKAASFEKNARVNLPKYQRRSP
                     ARKELVGVDMFVHWNGFDPDELASILQKIEKPNVKLNMITNRGIKVFPEGFKETFCTD
                     HWRCRFKPSEEGLISKNDIIELLSKADSNGIDVIKTENLYAFDGTPAFSLGQGQ"
     assembly_gap    7960..7977
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7978..8581)
                     /locus_tag="PK198_07570"
     CDS             complement(<7978..8581)
                     /locus_tag="PK198_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRF38630.1"
                     /translation="MTKITIAKGDGIGPEIMDATLDILMAAGADIETEEIEVGEKVYL
                     SGNTSGIAKASWDIIRRNKIFLKAPITTPQGGGYKSLNVSTRKFLGLFANIRPCKSLH
                     PFVATKHPNMDVVIVRENEEDLYAGIEHQQTDEVVQCLKLISRPGCEKIIRYAFEYAR
                     QQGRKKVTCFTKDNIMKQTDGLFHKVFNEIAAEYPDIENEH"
     gene            8675..9598
                     /locus_tag="PK198_07575"
     CDS             8675..9598
                     /locus_tag="PK198_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRF38631.1"
                     /translation="MNYTLNQLQVFLKIAETGSITKAAAELHLTQPAVSIQLKNFQDQ
                     FDIPLTELVGRRIYITDFGREIAQAAENILHQVQAINHKTLAYKGQLAGKLKLAVVST
                     GKYVMPYFLSGFIQQHQGVELRMDVTNRARVIESLVHNEVDFALVSIVPPDLKLESLD
                     LFQNKLFLIGNAEAKFKKSRSDKAVFNDLPLILREKGSGTRQTMEKFIEHNGITALRR
                     LELVSNEAVKQAVLAGLGYSIMSLVGIKNELRNKDLQIIPVEGLPIRTMWSLVWLQDK
                     RFSPVANAFLEYMRKEKSAIIESKFHWHEQY"
     gene            complement(9630..>9830)
                     /locus_tag="PK198_07580"
     CDS             complement(9630..>9830)
                     /locus_tag="PK198_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRF38632.1"
                     /translation="DYHIAIDMLNAMEIKSVRLLTNNPDKIEVFDNSGIELLDRVPLI
                     IAPHAENLNYLKTKQEEMGHLF"
     assembly_gap    9831..9851
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9852..10213)
                     /gene="ribA"
                     /locus_tag="PK198_07585"
     CDS             complement(<9852..10213)
                     /gene="ribA"
                     /locus_tag="PK198_07585"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HRF38633.1"
                     /translation="MTLQAEALIPTKWGNFRMLAFSDHPTNWMPHLALVHEKTDLTGP
                     VLTRIHSECITGDLFGSRRCDCGEQLYGAMEMIARDGGIIIYLRQEGRGIGIINKLKA
                     YNLQDLGLSTVDANIHLGL"
     gene            complement(10348..10674)
                     /gene="rplT"
                     /locus_tag="PK198_07590"
     CDS             complement(10348..10674)
                     /gene="rplT"
                     /locus_tag="PK198_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRF38634.1"
                     /translation="MPRSVNAVASRHRRKKILKAAKGYFGARSKVYTVAKNAVDKAMG
                     YAYRDRRSKKRAFRRLWIARINGVSYSKLMHLLATNGIDLNRKVLADLAMHHPQAFSA
                     VVKQVL"
     gene            complement(10753..10947)
                     /gene="rpmI"
                     /locus_tag="PK198_07595"
     CDS             complement(10753..10947)
                     /gene="rpmI"
                     /locus_tag="PK198_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRF38635.1"
                     /translation="MPKVKTNSSAKKRFSVTGSGKVKRFGANTSHRLTSKSRRAKLRN
                     RDSSIVNAGDMSLVRRMLWI"
     gene            complement(11017..11598)
                     /gene="infC"
                     /locus_tag="PK198_07600"
     CDS             complement(11017..11598)
                     /gene="infC"
                     /locus_tag="PK198_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRF38636.1"
                     /translation="MAKRFNDPDPNRAQYRINEQIRIPEVRLVGDSFEKISEVVGENV
                     EAGVYPTRKAREWAERMELDLVEISPNAVPPVVRITDFNKFLYDKKKKEKEIKAKAAK
                     TVIKEIRFGPNTDDHDFEFKVRHAKGFLEEGAKVKAYVQFKGRAIVFKDRGELILLRF
                     LKELEELGAAESLPRLEGRRMHVIITPKKAAKK"
     gene            complement(11714..12103)
                     /locus_tag="PK198_07605"
     CDS             complement(11714..12103)
                     /locus_tag="PK198_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HRF38637.1"
                     /translation="MLHKYIKSPHSSLGLDAWGMLASGLCAVHCLAIPVLLALGASAG
                     VAWLETPWLITAIGLAAQSLVGSYRREHGRPLALWVAGAGFAVLVASRLAPENLEHYL
                     TAVGGFAIATAHWINWKLRRQACLACR"
     gene            complement(12235..>13074)
                     /locus_tag="PK198_07610"
     CDS             complement(12235..>13074)
                     /locus_tag="PK198_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRF38638.1"
                     /translation="IWYRQGFPVKPPFLETNTQYFGSQVQALDFRNPDAKNVINGWVN
                     DKTRGLIPTIIDEIPDNAIMYLINAMYFKGSWTRKFDKDETTQMHFIKSNQTTTPVDM
                     MGYSGTVKFPLYTEDAFYAVDLPYGDSVYSMTLLVPRGNADVETLIADLNMQKWQQIT
                     TGMSVQEMNLRMPKFKMKYEKTLNRALQELGMQTAFSRAADFTNIADAELSVDEVKHK
                     SFIEVNEEGSEAAAVTSIGIIVTSVPSYPDILLNRPFLFVIRENQAGNVLFLGKMMNP
                     NEE"
BASE COUNT         2904 a         3291 c         3538 g         3292 t
ORIGIN      
        1 atattgcagg tatagaactg ccggacaatt gtattcccgg cgctgctccg gcccgcgaca
       61 ctattctctt ggatggcctg tcgggcactc acttcctgtt tgaggtcaat cttcaaaaca
      121 ccatttccaa tgagggtaca ctgcgcatca ccaacgaggc atacaccttg gatttgggca
      181 atgaaaaagg attggttctt ccttttaaac aactccgccg catcccggac tacaccatct
      241 ggggatatgc gggccatagc tctccatcgg cggcaggtat tgccggccaa ttcgtcaatg
      301 aactgtttgc ccgcagcacc gccgccaatc ttcaggcagg caattatggc tatttttccg
      361 tggcgccagg ctccggcagc atcaccgccg ccaacagccc cgccgacctg acactgcgtc
      421 cgttcgcatt tcgttacacg ggcaatctgg acgacttgcg cagcctcaca gaatcttatc
      481 gcaatcaata cggcgcgctt ttgcaaatca aattggttgc cgttacaggg gaggtttttt
      541 aggggtatca ataaacgaat aaatccggca actgccaggg attcagccgg ttgaggtcaa
      601 aagtaaaagc aatgcgttgc aggaaattgc cgcgttcggc caaacgggcg ctcagattct
      661 tggaattgat cagaggcaaa tagatgccga aaacaccctc tccaagctcc agcgccaacc
      721 cgccgctcca caaaatctgg tcaaccaaac ggtcattctg gcccgtaggc atggcattgt
      781 cgaaataccc gatatcgaaa tagggcttca gcggcaacaa aaactgccgg ggcaaatcgg
      841 cggccagatt cattgctatg ataaaattgt tacttctgcc cgcagcaaat cctgcaccgg
      901 tgagcgcttt catgccgccc tcccggaggc tgatttgttg agaccaaatg ccctcgcgtt
      961 cagtgcgccc gaagtagtag tcgtcaaaac ggtagtcgtt aaagccctgt gccgtgaggt
     1021 tgaacgcctc cggaaagatg gccccgcgtg tgcgcctgct gttgctcaaa aatccgccgg
     1081 caaaaacccg taaatgcaaa tgccgccctt tttcgtaagt aaaggcgcct ttccactctg
     1141 ccgatgcctt ccaatagttc aacggaatga actggtcttc atccttttga cgctctatgg
     1201 ccacgcgcca gctttggggg ttgacaaccc gccgctgctc gcccgcatag ctccattcct
     1261 gtatccaggc tgcctggcgc tgaagcccgc tgaatgtgcc gtcgggagca aattccgccg
     1321 actgtgccca ctgccggatg ccgcgccatt gcagtgtttg cgacagcgag gaggctgcgg
     1381 gccgatggag ttccaacttg acaaaaggcg tcaggcgggc gtattgcaga tggtaatcgt
     1441 ggcgctcgtt gtattcaaaa tgaaacatcc ggccgcccac gccgaagtga atgccatgca
     1501 atagctttcc cgcagggtac agatggtatt gtacagaacc tgtacctgcc aatgtgcctg
     1561 ttcgggtagc gtacatgggc gccgcggcaa attcaaaggg gcgctgcggc aaagtgctgt
     1621 tgtacaaggc gaggccgaac atattgccgt cgtactcatt ggcggctaat gccggggcaa
     1681 tgtgcaacac cgtgtggcgg tcgtcttcca accaaggcag caggcgcagg cgaagcggct
     1741 cgacgcgcct gaaaatgccg ctgctgcgca ggtggttgtt gcggcggttc atgtccggcg
     1801 aaagccgaag cgggtctatg ctgaaccggt cgtaatctcc ggcaggaaaa gtcagttctt
     1861 ttacgccttc aaaaccctcc acccattgcc cggctacgat ggagtctccc aacatagcgg
     1921 caatgtgcag cggcgcggcg atgcggcctt tattttggat gctcagacgc cattcgccgc
     1981 ctgttttgcg ccctttagaa atgcggtaat ccaatggccg gttggagccg atcagcccat
     2041 cgaagagcca gtgcaaatcc agcccgcttg tttgttccaa tacattgcgg aagtccttgg
     2101 gttgcggatg ccggaactgc cagatttgat aatattgctg catggcggca tccattttct
     2161 cctgcccgat gtacctttcg aggagttgca atgcccaggc gggtttgttg tacgcgccga
     2221 tgaagtagtt gaggccgcca aattcgttgg aagtggtggc tggggcctga tccatgcgtc
     2281 ggcgggcttg caggaggtag gccgcctcgt ttgtgcgcat ccctgatgct ttttttaatg
     2341 ccggccaaat gcccacatcg gaagaaccct cgccatagcg ctgcttcatg tagcgatgct
     2401 cgtagtaaga gttgaggccc tcgtcgagcc aggcatcgtc gcgttcatta ctggctaaga
     2461 tgccgtagag ccagttgtgc cccacctcat gcgcgatcac ttcatccagt gaaagcgcat
     2521 ttcccgctgc tccgatgatc gtaatcatgg gatactccat gccgccgccc gcactgagcg
     2581 caccttccac ggcagtagcc tgtggccagg gatactcgcc gagtgcggtg gaataaaact
     2641 gaaccgcttc ggccacatat tcggcgccgc gtttccacag atgcgcttca aaaggcgtaa
     2701 aaaatgccca ggcatccgcc gtttttccag atggaagcgc cagatttcgg tgctgcacca
     2761 tgaaacgctt gtcagcaaac caggcaaaat catgtacact gtcggcagta aagcggatgg
     2821 ttttggcgcg ctcggaggag gcggggaatg gcgcttcctg ccggcgttcg ggccagtcgg
     2881 cgagcaatgt ctccgtctct ttggtttttt ctttcaaaaa tgcctgctcg gtttcggtag
     2941 ccagtacgcc ggtggcagct accacataat tttcgggcac ggtaatttcc acctcaaaag
     3001 agccgaattc tgaataaaac tcgcccatat ccagataggg catggcgtgc caaccnnnnn
     3061 nnnnngaggc gaaggtcctg aagcgccacg tcgagcggcg tgccaaccgt cgcgatcata
     3121 cacggcgggc ttggggtacc attgggtgag ttggtaagat tgccctacat gtcccagccg
     3181 ggaaaaggag gccggaattt tgagaaaaaa cggcgtttcg atgctcacag aaccgccggg
     3241 cggcagggga gcgggcagtt cgaggcgggc caagtcggga ttttttgcat cggggtttaa
     3301 aagtgcgggg cgtccgtcgg cgcgaaaatc cagaccggag atgccgccca gagaggaatc
     3361 gggggcaaaa tgcagatcgg ctttgcgcaa tcgcagggct tgtttggcaa acgccgtgct
     3421 gcgcatgctg tatgcgttgg gccagaggtg ccaccagatt tcgcggagcg tatcgggcga
     3481 gtgattgacg tattgcagcg tcatgttgca ttgcaggctg tgctgccggt cgtccaatcg
     3541 ggccgctatg cgatagtgcg tttcttgctg gaaataagcg ggttgtgcaa gggtgctgcc
     3601 ggcacaaaaa gcaagcagcg ccgcgaacca ggttcgggcg ctgcccaaaa atgcagtctt
     3661 atggtatgcg atcatagttg atcgaatttt ttgaccaaat cactccagtt tttcaacatt
     3721 tcccggagct ttttgagtgt tcttagcttg ggccacccga aggagccggt ctcgatttct
     3781 acaaaagccg agtgtaccga ccctccgagt ggcgggtgca tttttctgag cctgaccttg
     3841 acgcccccca cttgcggaaa gaaatcattg atttcatcgg caatattttg ggcggttttt
     3901 tcaatcaggc tgctcgtatt tttcatctct cgctggcaga tcaggtaaat cgtttcgtag
     3961 ttgaccgtat gcatcaactc gtcggaatcg gcagcttctt tgatgttggt ttcaacataa
     4021 acatctatga tgaactccgt acccagaatt tcctcttccg gatagtaccc gtgcctgccg
     4081 tgcaggcgaa tgccttcgag tgcgatgatg gccatatcaa atcaagtgca gcaggatgac
     4141 agagttactt gttgtccttc acttcctcga actccacgtc ggtcacatct tctgccgcgc
     4201 cattgctgcc accttgctga tacgcgccgt tatccgatgc gtagtcctgc tgttgctgct
     4261 gctgttcctg cgtggcttgg tacatttcct gactggccgc ctgccaggcc gattccaatg
     4321 ttttggaagc gctgtcaatg cggtcgaggt cttgcatctt gtgcgcctct ttcaactctg
     4381 cggcagccga ttcgatggct tcttttttgt tggccggaat cttgtcgccg tactcgcgga
     4441 gttgcttttc ggtttggaac accagcgtgt cggccatatt gattttttct gcgcgttcgc
     4501 gggcctgacg gtcggtgtcg gcattggcgg cagcttcggc cttcatgcgt tctacctcgt
     4561 ctttcgacaa gccggtggag gcttcaatgc ggatgttctg ctctttgccg gtacctttgt
     4621 ctttggcggt cacgctgaga atgccgttgg cgtcaatatc gaaagagact tctacttgcg
     4681 gcaagccacg cggcgccggc ggaataccgt cgaggatgaa ccggcctacg gtgcggttgt
     4741 cgcgggccat ctggcgctcg ccttgcagga tgtggatttc taccgagggc tggttgtcgg
     4801 cagcagtaga atacaccttc gactttttgg ccggaatggt tgtgttggat tcaatcacca
     4861 cgtcatacac gccgcccagc gtttcgatac cgagggaaag cggcgtcacg tcgagcaaca
     4921 acacgtcttt cacttcgccg gtgagtacgc cgccctgaat ggccgcgccc acggctacta
     4981 cttcatcggg attcacgcct ttgttgggct ttttgccgaa gaaattttcc actgcctgct
     5041 gaatgcgcgg gatgcgggta gagccgccca cgagaatgat ctcgtccacc tgctctttgg
     5101 aaataccggc atcgcggagc gctgcttcgc agggcttgag ggtgcgctcc accaagtggt
     5161 ccaccaattg ctcaaacttg gcgcgagaca gcttgatgac caagtgcttg ggcacgccgt
     5221 ccactgcggt gatgtaaggc aggttgatct cagtctcgga agaggcagac agctcaattt
     5281 tggctttttc ggcagcatct ttgaggcgtt gcagtgcgat ggggtcttta cgcaggtcca
     5341 tattctcctg gcttttgaac tcatcggcca accagtcaat gatgatgtgg tcgaagtcgt
     5401 cgccgccgag gtgcgtgtcg ccgttggtgg atttcacctc aaacacgccg tcgcccaatt
     5461 cgaggatgga aatgtcaaag gtacctccac cgaggtcata cacggcaatg gtcatgtctt
     5521 tgtggcgctt gtccatgccg taagccaggg cggcagccgt cggctcgttg atgatgcgct
     5581 tgatggtaag gcccgcaatt tcgccggctt ctttggtggc ctggcgttgc gagtcgttga
     5641 agtatgcagg cactgtgact acggcctccg ttacaggctg tccgaggtag tcttcagcca
     5701 cttttttcat tttctggagt accatagccg atacttcctg cggcgtatag tggcgtccgt
     5761 caatgtccac gcgcaccgtg tcgttatcac ctttggctac tttataagga acgtgcgaaa
     5821 cctcgcccac gcgctcagag taacgggtac ccatgaagcg tttgatggaa aaaacggtgc
     5881 gttgggggtt ggtaatggcc tggcgtttgg ccggatcgcc tactctgcgt tctccgttgt
     5941 ccatgaaagc caccaccgag ggtgtagttc tgcggccttc atcgttcgga ataaccaccg
     6001 gctcgttacc ttccattaca gctacgcaag agttggtggt acctaagtca atcccgataa
     6061 ttttacccat gatatacagt ttgttgattt ggatgattca aaatgtcatc ctctccttac
     6121 aagctgtgtg ccaaaacaaa aaaatgcaga agccgtggga aatggcctgc caaatcaggt
     6181 aattattgtt ggacgatttt aataccgggg aatgtgggcg cggcagtgtg acaaaaaggc
     6241 agccgatttc gcaggaggga tgtatggggt gtcaagttgt acgatgaccg gcagagcaat
     6301 ggcgtctgtt gatgagacca ttaccctgcc ggatatgccg gggccgagca cttaatccat
     6361 catcaggttg atctcaacct ccttgcaagc ctgctcttgc ctgttggtaa tcaggccttt
     6421 gatctggatg ccgtgcttct tgatttcacg gcccagtacc tgggattgta cccaatgcgt
     6481 cgtctgttgt accatgctgt attcggccag cttttccact ttcgcttcgg tatccggcgc
     6541 ctccatgatg gcgtggcgat tgcgctgaaa aacttccaga ataggcttgc agacagttac
     6601 ttccggctgt tcctcctgtt cgctcacaac cgctcgaaga aaacggcacg atgtatcatg
     6661 cacgatgtaa atattatcgg ggttgttcct ggcaatgaaa ttcctgactt cctgcaagta
     6721 tggctcttcc tcaaatttga agtagccggc cggcgcggtc agaaagtaat tttcgttgcc
     6781 gtatttgctt ctgatgaatg gttcgacaga tgaaaacggg caaatcagga ataggttatt
     6841 gttggtcatt gtgtggatgg ttttatcgtt gaaaaataac tcaaatatgt tctatatgag
     6901 cctcttgcac cttgtacgca ggagcagact gccgcttctc ttcccgcttg cgggcaacag
     6961 gcgccggcat ggaggcgaac ctgaaaaaaa gcaggccgga tatgacagac aggctgatga
     7021 acaggcggct ttcaatgaga gagccagaag aaaaagcaag cgcgaggcag gctatgcacg
     7081 ctattgagca gagtatggct ttggctttca tgattaggaa atttgatgcg gtgatttgaa
     7141 ttattggcct tgccccagtg aaaaagccgg ggttccgtca aaagcgtaca ggttttccgt
     7201 tttgataaca tctatcccat tggagtcggc cttcgacaaa agttcgatga tatcgttttt
     7261 ggaaatgaga ccttcttcgg agggcttgaa ccggcagcgc cagtgatcgg tgcaaaaggt
     7321 ctctttgaag ccttcgggaa aaactttgat acctcggttg gtgatcatat tgagcttcac
     7381 gttgggcttt tcgatttttt ggaggatgga ggccagttcg tccgggtcga atccattcca
     7441 gtgcacaaac atgtccacgc ctacaagctc tttgcgggcc ggcgatctgc gctggtattt
     7501 gggcaggttg actctggcgt ttttctcaaa agaggcggct ttcagagcag ccggcttttg
     7561 gcccaggttg gaaatgaccg cctgcgcaaa acctctggtg cctactttca ctttgctgac
     7621 gccttctttg aaaacatcgg cggtgtgaac gccgtcttca atcgtcttga gccaggcgtt
     7681 ttggatggtt tctgctactt cggtttggcc aatatgatgg agcatcatca cggcagcctg
     7741 caacagtccc gatgggttgg ctatgttttg gccggcaatt ttgggagcag agccgtgtat
     7801 ggcttcaaac atcgaacact cttcaccaat gttggcagaa ccgcccaacc cgacggagcc
     7861 ggtgagctga gcggccacgt cggagagcac atcgccatac aaattggccg tcaccactac
     7921 gtcgaagact tcgggcgtat ctgccagcat ggccgctccn nnnnnnnnnn nnnnnnnagt
     7981 gctcgttttc aatatcggga tactccgccg caatttcatt gaacaccttg tgaaacaagc
     8041 cgtcggtctg tttcatgatg ttgtctttgg taaagcaggt taccttttta cgaccttgtt
     8101 gacgagcgta ctcgaatgcg taacgaatga ttttttcaca accggggcga ctgatgagtt
     8161 tgaggcattg cactacctca tcggtttgct gatgctcaat gcctgcgtac aggtcttctt
     8221 cattttcgcg cacaatcacc acgtccatat tggggtgttt ggtggccaca aaaggatgca
     8281 ggcttttgca ggggcggata ttggcaaaca gacctaaaaa cttgcgcgta ctcacattga
     8341 ggcttttgta tcctcctccc tgcggcgtag tgatcggggc tttcagaaaa attttattcc
     8401 tcctgataat atcccaggat gctttggcaa tccccgaagt gttgcccgaa aggtaaacct
     8461 tttcgcccac ctcaatttcc tcagtttcaa tatcagcgcc ggcggccatg aggatatcaa
     8521 gggtggcatc catgatctcc ggaccgattc cgtctccttt ggcaatggtg attttggtca
     8581 tgattgagta ttttttggtt caacgacaca aagttgcggg gggaagttca tttgtcttca
     8641 tttatatttg caatgaaatt cataacatat ttttatgaac tatacattga accagcttca
     8701 ggtcttcctg aaaattgccg aaaccggcag tattaccaag gctgcagcgg aattgcacct
     8761 cacccagcct gcagtgtcta ttcagttgaa aaattttcag gatcagtttg acattcctct
     8821 tacggagttg gtaggaagaa gaatctacat cacagatttc gggcgggaaa tcgcacaagc
     8881 cgctgaaaac attctacatc aggtgcaggc aatcaaccac aaaacattgg cttacaaagg
     8941 ccagttggcc ggcaaactga agctggccgt ggtttccaca ggaaaatatg tgatgcctta
     9001 ttttttatcc ggctttatac agcagcatca gggggtagaa ctgcgaatgg acgtgaccaa
     9061 ccgggcaagg gtgattgaaa gcctcgtaca caatgaggtg gattttgcat tggtatccat
     9121 cgtacctccc gatctgaaat tagaaagctt ggacttgttt caaaacaaac tatttttgat
     9181 aggaaatgcg gaggcgaagt tcaaaaaaag ccggagcgac aaggctgtat ttaatgattt
     9241 accgctcatc ttgagagaga aaggatcggg gacaaggcaa accatggaaa agtttattga
     9301 acacaacggt attacagcac tgaggaggtt ggaactcgtg tctaacgagg ccgtcaagca
     9361 ggcggtgctg gcaggcttgg gctattccat tatgtctctg gtcgggatca agaacgaact
     9421 gcgcaataag gatttacaaa taatcccggt agaagggctt cccatccgga ctatgtggag
     9481 cttggtgtgg ttgcaagaca aaaggttctc gcccgtagca aatgcgtttc tggagtatat
     9541 gagaaaggaa aaatcggcca tcattgaaag caagtttcac tggcacgaac agtattagcc
     9601 tgtttcggca ataaccgatg gtggacagat tagaaaagat gccccatttc ttcctgtttg
     9661 gttttgaggt agttgaggtt ttcggcatgc ggcgcaatga tgagcggcac ccggtccaat
     9721 agctctatgc cggagttgtc gaatacctcg atcttatcgg ggttattggt cagcaatctt
     9781 accgacttga tttccatagc attcagcatg tctatggcga tgtgataatc nnnnnnnnnn
     9841 nnnnnnnnnn naggcccagg tgaatgttgg cgtcaacagt gctgaggccg aggtcttgca
     9901 ggttgtacgc cttgagtttg ttgatgatgc cgatgcccct gccttcctgc cggagataaa
     9961 tgatgatgcc gccgtcgcgg gctatcattt ccatagcgcc atacagttgt tcgccacagt
    10021 cgcagcgccg cgagccgaag aggtcgccgg tgatgcactc cgaatggatg cgggtgagga
    10081 cagggccggt caggtctgtt ttttcgtgaa caagtgcgag gtgcggcatc cagttcgtcg
    10141 gatggtcgga gaaggccagc atccggaaat tgccccattt ggtggggata agcgcttcgg
    10201 cttgtagcgt cataacgatg gattcaaaat acaatgcaaa gaaaacgcaa aagcggtcaa
    10261 atcgtttgcc gtacgacttt gcgccaatca aaaacggcta agaggagtac cccttagccg
    10321 tttcgatcac aaaaataaag cgtactatca gagaacttgc ttcaccacgg cagagaaagc
    10381 ctgcgggtgg tgcatggcca agtcggcaag tactttgcgg ttgaggtcta tgccgttggt
    10441 agcgagcaag tgcatgagct tggagtagga tacgccgttg atgcgggcaa tccaaaggcg
    10501 gcggaaagcg cgttttttgc tgcggcggtc gcggtaggca tagcccattg ctttatccac
    10561 agcgtttttg gctacggtgt aaactttact gcgcgcaccg aaatagccct tggcagcttt
    10621 gaggattttt ttgcgccgat gccgggaggc aacagcattc actgaacgtg gcatattgag
    10681 ttgtttttag tgcaatgcag ggttccggtt gtatcagaaa cttcggcctg cattctcgtt
    10741 aaaaaataaa aattagatcc agagcatacg tcttaccaac gacatatcgc cggcattgac
    10801 gatgctggag tcgcggttgc gcaatttagc tctacggctt ttgctggtga ggcggtgcga
    10861 ggtgttggcg ccaaaacgct ttaccttgcc ggagccggtc accgaaaagc gctttttagc
    10921 gctcgaattg gtttttactt tcggcataat tgtggttttt taaaacggaa cgcaaaggta
    10981 agtcgctttg caggaaaaaa caaaagccga tgcagattat tttttagcag ctttcttggg
    11041 cgtaatgatg acgtgcatcc tgcggccttc caaacggggt aaagactcgg cagcgcccaa
    11101 ctcttccaac tctttgagga agcgaagcaa aatcaactcg ccgcggtctt tgaacacaat
    11161 ggcgcgccct ttgaactgca catacgcctt tactttggcg ccctcttcga ggaagccctt
    11221 ggcatggcgc actttgaact caaagtcgtg atcgtcggtg ttggggccaa acctgatttc
    11281 tttgatcaca gtcttggcgg ccttggcctt gatctccttc tccttcttct ttttgtcgta
    11341 caaaaacttg ttgaagtccg tgatgcgcac cacgggcggc acggcattag gggaaatttc
    11401 cacaaggtca agttccattc tttctgccca ctccctcgct ttcctggtag gatatacgcc
    11461 tgcttcaacg ttttcgccta caacctcgct tattttttcg aagctgtcgc ccaccaaacg
    11521 gacttcgggg atgcggattt gttcgttgat ccggtactgt gccctgttgg gatccggatc
    11581 gttgaatctc tttgccaaat gttgttgttt taatgaaata aaaatctgct ttcagcccgc
    11641 gaagttcaca actttttcgc aaactaaaca agtaagagga gataaaatat taagaacccg
    11701 gagttttgaa cgattaccgg cacgctaagc aggcttgccg gcgcaatttc cagttgatcc
    11761 aatgggccgt agcaatggca aatccaccta ctgcggtcag gtagtgttcg aggttttcag
    11821 gggccaggcg gctcgccacc aataccgcaa aacctgcccc ggccacccac agcgccaaag
    11881 gccggccatg ttcgcgccgg taagacccga caagagactg cgccgccaaa ccgatggcgg
    11941 ttatcaacca cggcgtttcc aaccaggcta cgcccgctga tgccccgagt gccagcaaaa
    12001 caggaatggc cagacaatgc acggcgcaaa ggccggatgc caacatgccc caggcgtcta
    12061 atccgaggga tgaatgaggt gattttatat atttgtgcaa cattgttgca aatgtagcgg
    12121 gttttgtctt atctgtacaa gtaaaaggag ggattttttt taaatggggt aaaaaaggcc
    12181 cgctgccaca ctttgacaac gagccttttc ttctttattt aaggaataaa tgacctactc
    12241 ctcgttcggg ttcatcatct tgccgaggaa cagcacattg cctgcctgat tctcgcggat
    12301 gacaaacagg aaggggcgat tgagcagaat gtcgggataa gaaggaacgg aagttacaat
    12361 gatgccgatg gatgtgacag cggcggcctc cgagccttct tcgttcactt cgatgaagga
    12421 tttgtgtttt acctcatcca ccgagagttc tgcatcggca atattggtaa agtcggcagc
    12481 gcggctgaaa gcggtttgca tgcccaattc ctgcagcgcc cggttcagcg ttttttcgta
    12541 cttcatcttg aatttaggca tccgaaggtt catttcctgc acgctcatgc cggtggtgat
    12601 ttgctgccat ttttgcatgt tcaggtcggc gatgagcgtc tccacatcgg cattgccgcg
    12661 aggtacgagc agggtcatgg aatagacgga atcgccgtaa ggcaaatcaa ccgcataaaa
    12721 cgcatcctcg gtgtagagcg ggaattttac ggtgccgctg taccccatca tgtccaccgg
    12781 cgtagtggtc tgattgctct tgatgaaatg catttgcgtg gtctcgtctt tgtcgaattt
    12841 gcgcgtccag gagcctttga aatacatggc attgatgagg tacatgatgg cattgtcggg
    12901 aatttcgtca atgatggtag gaatcagccc gcgggttttg tcgttcaccc agccgttgat
    12961 gacgtttttg gcgtcgggat tgcgaaaatc gagggcctgt acctgactgc cgaagtattg
    13021 ggtattggtt tccaaaaacg gcggtttgac cggaaagccc tggcgatacc atat
//
LOCUS       DAORLF010000066        13050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2555_length_13050_cov_1.905810, whole genome shotgun sequence.
ACCESSION   DAORLF010000066 DAORLF010000000
VERSION     DAORLF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_13050_cov_1.905810"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>240
                     /locus_tag="PK198_07615"
     CDS             <1..>240
                     /locus_tag="PK198_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38639.1"
                     /translation="KMNRQISQLRGQMHKLQEIIHNNKKQIESNLQLASKARETNKQN
                     VMILKSRQAGRLSESNLKLEDLYRKMEILYRVLTKM"
     assembly_gap    241..250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <251..699
                     /locus_tag="PK198_07620"
     CDS             <251..699
                     /locus_tag="PK198_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38640.1"
                     /translation="NSQVLMEDIQDQVMVKEQERKAIHASYGAMKSAMSIMSGDPDKR
                     AMFDMAMESIAEDVAGKVGEMEQFMEMSSSFMNSIDLQNGIFEEEGLKMLEKWESEGV
                     SLILGKEKDSLLLQANNPNEVLDLNVPIKQAVREPGHRNQYDSLFD"
     gene            721..2289
                     /locus_tag="PK198_07625"
     CDS             721..2289
                     /locus_tag="PK198_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HRF38641.1"
                     /translation="MAQKLTTFSKFLIVALVVGGAAFAVKYLQSKGIIGNESSSDTGK
                     KTERNETNSKYKNDDVVKVGVVTWGGYAGGQYFNEGFEAGEKSRFTKEYGIPVEFQVL
                     DDFEASRNAFRSDEVNLLWTTADAFPTEAGGLSDYDPVIVFQADWSRGGDACVVRRGI
                     TKVSDLKGKKIAVAEMTPSHSFLLWLLDAGGISAKDVEIVPQASAIDAAEVFKSQKVD
                     AAVVWSPDDILCTKAVPGSRILESTRSATNIIADVFIAKRAWAEANRDKLQKLYEGWM
                     IGAAEITSDPAAKRKAAEILAREFTDRGTPLSNEESMQMLDNVRLCTHGDNRNFFGLN
                     SDYKGVNGNALYSHMSGTYSSLGYMEASSVPNWRRVAFPSLVTSANLSGTEHNAEGQK
                     QFTKVTTDEGKDREAFASKQVSISFRSGEFQLDENAKYIIDKEFVEIAKAFGSARIRI
                     EGNTDNVGSRESNVALSQKRAQSVANYLVREHGMSSNRFIVVGNGPDKPVAPNTTDDG
                     RSKNRRTDFELVKD"
     gene            complement(2279..3364)
                     /gene="wecB"
                     /locus_tag="PK198_07630"
     CDS             complement(2279..3364)
                     /gene="wecB"
                     /locus_tag="PK198_07630"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRF38642.1"
                     /translation="MQIKKILLLIGTRPEAIKMAPLIRELRSRPQHCEVWAAGSGQHK
                     DTVREALALFGIHLDAEIPWQASAHLIELHTRLLSACHALIGQFQPDMVLVQGDTSTA
                     FAGAWAAFYSGIPVGHVEAGLRSGSLESPFPEEMHRIQIARLAAWHFAPTESARQNLL
                     SEGISPDRIWVCGNTGIDALHIALEKIRKGGTASAVDSNKIKITATLHRRENQPFLEK
                     ICRDLLELLQNERFELDFVLHPNPAVQARVRPILEGHACIRLHPPLDYLRFLQLLDGS
                     SLIITDSGGIQEEAPTLGKPVVVLRSHTERTELIESGGGILCDPLTGNLQEAVQQAEN
                     NIAKETPENPFGDGRAARRIAEVVINL"
     gene            3374..4276
                     /locus_tag="PK198_07635"
     CDS             3374..4276
                     /locus_tag="PK198_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38643.1"
                     /translation="MKKIAIVHKGKAYIPEARVYQDLLSQYGFQVAYCRTNEELAAEN
                     YDVEWHIMGADLPSFSKKDRFVIHEYLSLSVPPMARLKNQMKKLLNRRPNLRIFNGAA
                     IQSELDFQDEVRHIIRNPGLSKDFFVDGQSEKAHDFVYLGAMDRRRNIPVFFDWVCRQ
                     LPGSSILAIGTPPPVADLSKRYPNIHFVGQVSHNEIPALLRTAECGLNLMPDLYPYHL
                     QPSYKLIEYCAAGLKVITTDYLWANQFEEQHQARFFKLNRRFDNLHLEALSRFPFVTP
                     DVSQLTWEHSFRQSGLLDLLLSIH"
     gene            complement(4262..5554)
                     /locus_tag="PK198_07640"
     CDS             complement(4262..5554)
                     /locus_tag="PK198_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38644.1"
                     /translation="MRILLLTTQYHPAANPNVFRWAAIAGHWVAAGHEVHLLCAEHSQ
                     RPREETIQGVQVHRAGQATLMDWTYNLLKTKHRRSEPGTGAGRAGWGRSVLETLLNLT
                     WRKLYWPDGSCLWYLPARRRAVQLLQQHDFDALISVGTPFTCHWVALACKKRFPKLRW
                     LMDVEDPFSIAEEFPNNNFALYRRRNHRAEALAFRSADAVAVTNPAVLRRYAAVFQED
                     AHKMSVIPPLYDPAVPSDYSLPPRLPGEIRLGYFGSFYHKIRTPQRFLEMLEIAVGQS
                     GGALSQSLSVHFFGYLEPEFTAMFERFHSLSPHIRLHGLLSREDAAAAMQQMDALLHI
                     GNSTDYHLPSKAPEYLFSGLPVVNIAACVSDTFTEFAGVRAALLHIPPDVELPEAVSR
                     LSGFLLHLPKGDLPGDEEVESVGVAAVAGRYEAALMDR"
     gene            complement(5562..6413)
                     /locus_tag="PK198_07645"
     CDS             complement(5562..6413)
                     /locus_tag="PK198_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38645.1"
                     /translation="MIRVLPTIVFAICCQSTALAGIISGDTTLCTSFDMSEGYAGWQF
                     SGMLQRQLVPAEGENSNFIRITDRLGPVSAGIAPPVFHGDWSLWNERGFVQFDIKIEP
                     SNPADTLLSGYPVLLLSGPGGSAQINAWPSDLLSAIGQWQRLSFALNEADWTMLSGDW
                     AALMQHVTRVQVTLEFIYGSEIVWFDNFCLHRPALELLTGKPTEDDIRVFPNPAFERK
                     ITISSTAPSLAGSLSVWDIHGRRIVYVADQELNGYEWDGGSLPAGTYFLLIQQSGRQV
                     PVKVIFQ"
     gene            6527..>6721
                     /locus_tag="PK198_07650"
     CDS             6527..>6721
                     /locus_tag="PK198_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38646.1"
                     /translation="MRTLSAIIFFWIGLVGATAQDGARNKGNAALFHISYAFQLPAGD
                     LSERYGSNTNVGIAAEWMTDK"
     assembly_gap    6722..6771
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6772..7290
                     /locus_tag="PK198_07655"
     CDS             <6772..7290
                     /locus_tag="PK198_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38647.1"
                     /translation="GNQVKTDVLAGLRTAEGFIVGNDRGIADIPLKQRGYYIGGMAGK
                     LFPMSAKETRSGLRLTLGAGLLQHKIRIQDDPERGVAALAGDYKKGYDRLSNGLAFNA
                     FAGYQMLSNDGRINFFIGLEACYGMVQNRRSFNYDTRTQDTRRYNDALIGLRAGWTLP
                     FYVGKASGEIFY"
     gene            7297..>7591
                     /locus_tag="PK198_07660"
     CDS             7297..>7591
                     /locus_tag="PK198_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRF38648.1"
                     /translation="MLLLWLYDAGIALYVAALRIAAVFHPKARKWVQGRTAWEARLKS
                     EIAEKFSPGKPRLWMHCASLGEFEQGRPVLEAWRKEHSDTQILLTFFSPSGYEI"
     assembly_gap    7592..7607
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7608..8567
                     /locus_tag="PK198_07665"
     CDS             <7608..8567
                     /locus_tag="PK198_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRF38649.1"
                     /translation="EIRKDYPGADIVAYLPTDTPRAARRFVELVQPDWVIFVKYEFWL
                     RTLFALFRRNIPVLLISAVFRPGQIFFKGYGGLWKKALSEFRHIFVQDAASMELLQRH
                     GIQRCSIAGDTRVDRVVSIAEEGREIEIVKHFTAGHKVFIAGSTWPPDEELLAVLLRE
                     NLLPGWKFIIAPHQISESNLRRMEALLPAESVRFSAINPAQPLPQAVLLIDNIGMLNA
                     LYRYGDLAYIGGGFGAGIHNTLEPIAFGLSVIFGPKYKKFREAVEMVQTGGAVSVNNA
                     EDFCRAIVHFAEENHRRQARQAALQYLRDNAGATQKILGRPGL"
     assembly_gap    8692..8701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8702..>9447)
                     /locus_tag="PK198_07670"
     CDS             complement(<8702..>9447)
                     /locus_tag="PK198_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF38650.1"
                     /translation="FNTLQMGLVASERVFALLDRKEFIANTGKLKPERFRGDLTFEDV
                     SFAYNDEDFVLKDVNFHISAGQTLAIVGSTGSGKTTTMSLLTRFYDIQQGHIKVDGHD
                     IRDYELHAYRRRIALVLQDVFLFSGTVMENITLRDASISRERVIEAAQMIGAHEFIEK
                     MPGGYEYQVMERGATLSMGQRQLISFVRALVFDPDILILDEATSSIDPETESVIQFAI
                     ETLIAKRTSIIIAHRLSTIRRADRILVITK"
     assembly_gap    9448..9580
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9581..>9940)
                     /locus_tag="PK198_07675"
     CDS             complement(<9581..>9940)
                     /locus_tag="PK198_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF38651.1"
                     /translation="EGIITIIADLLSLIAVLGVMFYTSWKLTLVCMITLPFLVAAAYV
                     FKEKVKASFQVVRNQISKMNAFMQERITGMRIVQIFNAEEQEAQRFRAINREYTKANL
                     DAILYYAVFFPVVEIIAA"
     assembly_gap    9941..9974
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9975..10363)
                     /locus_tag="PK198_07680"
     CDS             complement(<9975..10363)
                     /locus_tag="PK198_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF38652.1"
                     /translation="MADNTAPTRKMPLLGRVLALAKPYAALFAATTLLALLLAPLSVL
                     RPYLIKVMVDEYIAPDKVGQLTQIAIFLIVLLIVEAGLQYVFQYGTSWLGQGVVRDLR
                     VRVFQHITNLRLSYFDRTPIGTSTTRT"
     gene            10531..10923
                     /locus_tag="PK198_07685"
     CDS             10531..10923
                     /locus_tag="PK198_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF38653.1"
                     /translation="MNTQNILSWLLRVAAAAILLQTLYFKFTAHPQSVELFSLLGVEP
                     WGRIGTGIIELITGILLLIPTTAFVGAFLGLMSGAILSHLVFIGIESHGDGGLLFGLA
                     IAVFVMCAAIMWLHRGQGAALYLWVRNK"
     gene            11036..>13050
                     /locus_tag="PK198_07690"
     CDS             11036..>13050
                     /locus_tag="PK198_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF38654.1"
                     /translation="MSSLSQNIRCLVFLFLMAACLIPAMTSAQQKVLIIGIDGCRHDA
                     LRAAYTPQLDSLIAESVYSYDALTEEPTWSGVGWSAMLTGVWRNKHGVTDNSFTGNHF
                     ADYPHFMKRVEAFDPSLFTVSISHWAPINTQIVGGGGIDLILSPPTDSLVSVAAVDVL
                     RNQNPDVIFLQFDDVDHAGHASGFSMNVPAYMAAIEAVDRFVGYIMAALKSRPNYAGE
                     DWLIIGSTDHGGIGTSHGGASFVERNIFYFASKAGRTATEMAKTAVLTNSNCMPDTLG
                     LRMAGATDYASVPNNALFQFGTSQDFTIELRIKTAGWTGDPSFVGNKNWNSGSNKGFV
                     ISSPLNNQNIWKVNIGDGRNRADVNGSAIADNRWHHLAAVFDRDGLLRLFKDGMPDGS
                     TNMAHVSGNINNTLALGIGQDGTLGYGSAMNGIISEVRIWKTAVNEATLAAWQCTPIH
                     SSHPNYADLAGYWRINEGNGQQLADSGPNGWHALYHGNAADWEPVNLPDTTYDFSNTP
                     RIVDVATTALAHLCVPVQEAWELDGRVVGVEIPQPHISGSPLACEHTQEMYSLTTNGL
                     QPIRWSVTNGVILSGADSSAVLVQWNTAGAGSLTAEQCNVQAVFPVTVDVCSASSEMQ
                     PPAPYSLYPNPTQGLFTVSAAEAASIRRVGVRDALGRELLVAQPVSG"
BASE COUNT         2999 a         3634 c         3410 g         2754 t
ORIGIN      
        1 aaaatgaacc gccagatcag ccaattgcgc ggccagatgc acaagttgca ggaaatcatc
       61 cacaacaaca aaaagcagat cgagagcaac ctccaattgg ccagcaaagc ccgcgagacc
      121 aacaagcaaa acgtgatgat cctcaagtcg cgccaggccg gccggctcag cgaatccaac
      181 ctcaaattgg aagacctcta tcgcaagatg gaaatcctct accgggtact caccaaaatg
      241 nnnnnnnnnn aaaactcgca ggtgctgatg gaagacattc aggatcaggt gatggtgaag
      301 gaacaggagc gcaaagccat tcacgccagc tacggcgcca tgaaatcggc gatgagcatc
      361 atgtccggcg accccgacaa acgcgccatg ttcgatatgg cgatggaatc cattgcagaa
      421 gacgtggccg gcaaagtggg cgaaatggag cagtttatgg aaatgtcgtc cagctttatg
      481 aactccattg acctgcaaaa cggcattttt gaagaagaag gcttgaaaat gctggaaaag
      541 tgggaatcgg aaggcgtctc gctcatcctc ggcaaagaaa aagactcgct gctgttgcaa
      601 gccaacaacc ccaacgaagt gctcgatctc aacgtgccca tcaagcaggc ggtgcgcgaa
      661 ccgggccaca gaaatcaata cgattcactt tttgattaac ctcaaacaac tcgcatcatc
      721 atggcacaga aactcaccac tttttccaaa ttcctcatcg tcgcgctggt cgtcggaggc
      781 gctgctttcg ctgtgaaata cctccagagc aaaggcatca tcggcaatga atcgagtagc
      841 gataccggta aaaaaaccga gcgcaacgaa accaattcca aatacaaaaa cgacgatgtt
      901 gtcaaagtcg gcgtcgttac ctggggcggt tatgccggcg gtcagtattt caacgaaggt
      961 tttgaggccg gcgaaaaatc ccgcttcacc aaagagtacg gcatccctgt ggaatttcag
     1021 gttttagacg actttgaagc ctcccgcaat gctttccgct ccgatgaagt caacctgcta
     1081 tggactaccg ctgacgcatt tcccaccgag gcgggcgggc tttccgacta cgatccggtc
     1141 attgtttttc aggccgactg gtcgcggggc ggcgatgcat gcgtcgttcg gcgcggcatt
     1201 accaaagtgt ctgatctgaa aggcaaaaaa attgccgtag cggaaatgac gccctctcac
     1261 tcgttcctcc tttggttgct cgatgcgggc ggtatttctg ccaaagatgt ggaaatcgtt
     1321 ccgcaggcca gcgccattga cgccgccgag gtgttcaaaa gccaaaaagt ggacgctgcc
     1381 gttgtgtgga gtcccgacga tattttgtgt accaaagccg tgccgggttc cagaattttg
     1441 gaaagcaccc gctcggccac caacatcatc gccgacgtgt tcatcgccaa gcgcgcctgg
     1501 gccgaagcca atcgcgacaa gctgcaaaag ctttacgaag gctggatgat cggcgcggcg
     1561 gaaatcacct ccgacccggc cgccaagcgc aaagccgccg aaatcctggc gcgtgaattt
     1621 accgaccggg gcaccccgct ctcgaacgaa gaatccatgc aaatgctcga caacgtgcgc
     1681 ctgtgtacgc atggcgacaa ccgcaatttc tttggtctga acagcgatta caaaggcgtg
     1741 aacggcaacg cgctctactc gcacatgtcc ggcacctatt ccagcttggg ctatatggag
     1801 gcttcctcgg ttcccaactg gcgcagagtg gcgttcccgt ctttggtgac gtctgccaac
     1861 ctgagcggta cggaacacaa tgccgaaggc caaaaacagt tcaccaaagt gaccaccgat
     1921 gagggcaagg accgcgaggc atttgccagc aagcaggtgt ccatcagttt ccgcagcggc
     1981 gagtttcaat tggatgaaaa cgccaaatac atcattgaca aggagtttgt ggaaattgcc
     2041 aaggcgttcg gcagtgcgcg catccgcatc gaaggcaaca cggacaatgt gggcagccgc
     2101 gagagcaatg tggcgctttc gcaaaagcgg gcgcagtcgg tggccaacta tttggtgcgc
     2161 gagcatggca tgtcgtccaa ccgcttcatt gtggtgggca acggccccga caaaccggta
     2221 gcccccaata ccaccgacga cggtcgctcg aagaaccgcc gcacggattt tgagttggtt
     2281 aaagattaat caccacctcc gctatccgcc gcgcagcccg tccatcgcca aagggattct
     2341 caggagtctc tttggcgatg ttgttttcgg cctgttggac ggcctcttgc aggttgccgg
     2401 tcagtgggtc gcagaggatg ccgccgcccg attcgatcag ttccgtgcgt tcggtatggc
     2461 tgcgcaacac caccaccggt ttacccagcg tgggcgcttc ttcctgtatg ccgccactgt
     2521 cggtgatgat gagggaagag ccgtccaaga gttgtaaaaa gcgcagataa tcgagcggcg
     2581 ggtgcaggcg aatgcaggca tggccttcca aaatgggccg cacccgggcc tgtacggcgg
     2641 ggttgggatg cagcacaaaa tccaattcaa agcgctcatt ttgaagcaat tcaagcaaat
     2701 cccggcagat tttttctaaa aaaggttgat tttcccgccg gtgcaacgtc gccgttattt
     2761 tgattttatt ggaatcaact gccgaggccg taccgccttt tctgatcttt tccagcgcaa
     2821 tatgcagcgc gtcaatgccc gtattgccgc agacccaaat ccggtcgggt gagatgccct
     2881 ccgacaacag gttctgccgg gccgattcgg tgggcgcaaa atgccatgcc gccaaacggg
     2941 cgatctgtat gcggtgcatc tcttccggaa acgggctttc caaagaaccg ctgcgcaaac
     3001 cggcctccac atgtcccacc ggaatgccgc tgtaaaacgc cgcccatgcg cccgcaaaag
     3061 ccgtgcttgt atcgccttgc accagcacca tatcgggttg gaactgcccg atcagcgcgt
     3121 gacaggccga cagcaggcgc gtgtgcagtt cgatcagatg agcagaggct tgccagggga
     3181 tttctgcgtc taagtgaatc ccaaacagcg caagcgcttc ccgcaccgta tctttgtgct
     3241 gcccgctgcc cgccgcccat acttcacagt gctgtgggcg gctgcgcagt tcccgtatca
     3301 gcggcgccat tttgatggcc tccgggcggg tgccgatgag gaggaggatt tttttaatct
     3361 gcatacatct tgaatgaaaa aaattgccat tgttcacaaa ggtaaggcgt acatcccgga
     3421 ggcaagggtt tatcaggact tattgtcgca gtatgggttt caagttgcat attgccgaac
     3481 aaatgaggaa ttggccgccg aaaattatga tgtcgaatgg catatcatgg gcgccgatct
     3541 tccatctttt tcaaagaaag acaggtttgt gattcacgaa tatctatcgc tttcggttcc
     3601 cccaatggcg cgcttgaaaa accagatgaa aaagctgctc aacagacgcc ccaacctcag
     3661 aattttcaac ggggctgcca ttcagtccga acttgatttt caggatgaag tacggcatat
     3721 tatcagaaat ccggggctga gcaaagactt cttcgtagat gggcaaagtg aaaaagcaca
     3781 cgattttgtt tatctcggcg ccatggaccg ccgcagaaac attcctgtat tcttcgattg
     3841 ggtttgcagg caattgcccg gcagttccat ccttgccatc ggcacgccgc cgcccgtcgc
     3901 ggatttgtca aaaagatacc ccaatatcca ttttgtcgga caagtatctc ataatgaaat
     3961 acctgcactc ctccggactg ccgaatgcgg cctcaatctg atgccggatt tgtatccgta
     4021 tcacctacaa ccctcttaca aactgatcga atactgcgcc gcaggattga aggtaattac
     4081 aacggattat ctctgggcga atcagtttga agaacaacat caggcgcgat ttttcaaact
     4141 caatcgcagg tttgacaatc ttcatttaga agcgttatct cgtttccctt ttgtcacccc
     4201 ggatgtttct cagctaacct gggagcatag ttttcggcaa tccgggctgt tggatttact
     4261 cttatctatc cattaatgcc gcctcatacc tccctgccac ggccgctacc cccacgcttt
     4321 ctacctcctc atcgcccgga agatcgcctt tcggcagatg cagcaaaaaa ccgctcaacc
     4381 gactaaccgc ttccggcaac tccacatccg gcggaatgtg cagcaaggca gcgcgcacgc
     4441 cggcaaattc tgtaaaagta tctgacacac aggcagctat gttcacaaca ggcaggccgc
     4501 tgaagaggta ttccggggct ttgctcggca ggtggtaatc ggtcgaattg ccgatgtgca
     4561 gcagcgcgtc catctgttgc atggcggcgg cggcatcttc ccggctcagc aaaccgtgca
     4621 gccggatatg gggcgacagg ctgtgaaagc gttcaaacat ggccgtaaat tccggttcca
     4681 gatacccgaa aaaatgcacg ctcaagcttt gcgaaagcgc ccctcctgac tgaccgaccg
     4741 caatttccag catttccaaa aagcgctgcg gcgttcggat tttgtgataa aacgagccaa
     4801 agtagcccaa cctgatctct cccggcaggc gcggcggcaa actgtaatcg gacggaaccg
     4861 ccggatcata cagcggggga ataacgctca ttttgtgcgc atcctcctga aatacagcgg
     4921 catacctccg cagtactgcc ggattggtga cggccaccgc atccgctgag cgaaacgcaa
     4981 gagcctccgc ccggtggttc cgtcggcggt acagtgcaaa attgttgttg ggaaactcct
     5041 ccgcgatgct gaacgggtct tcgacgtcca tcagccagcg cagtttcgga aaacgttttt
     5101 tgcaggccag cgccacccaa tggcaggtga acggcgtgcc cacactgatg agggcgtcaa
     5161 aatcatgttg ctgtaacaat tgcacggctc tgcgccgcgc cggcaggtac cacaggcaac
     5221 ttccatcggg ccagtagagc tttcgccaag tcaggttcag cagcgtttcc agcacgcttc
     5281 tcccccaccc tgctctaccg gcgcctgtgc ccggttcgct gcgccgatgt ttggttttca
     5341 acagattata agtccaatcc atgagcgtag cctgcccggc gcgatgcact tgtacgccct
     5401 gaatcgtttc ttcgcgaggt cgttggctgt gttcggcaca aagcaaatgc acctcatggc
     5461 cggcagcaac ccaatgacct gcaatggccg cccagcggaa gacgttggga ttggccgccg
     5521 ggtggtattg ggtggtgagg aggaggatgc gcatggcttc cttactggaa aatcaccttt
     5581 acaggaacct gccgaccgct ttgctgaatc aataaaaagt aggttcccgc aggcaaactg
     5641 ccaccatccc attcatatcc attcagctct tgatcagcaa catacacgat tcttcttccg
     5701 tgaatatccc acaccgacaa agaacctgcg agcgacggcg ccgtagaact gatcgtgatt
     5761 tttcgctcaa aagcaggatt gggaaatacc cgaatgtcat cctccgttgg ctttccagtc
     5821 aataattcaa gtgcaggccg atgcaggcaa aaattatcga accagacgat ttccgatcca
     5881 taaatgaact ccaaagtaac ctgaacacgc gttacatgtt gcatcaaagc ggcccaatct
     5941 ccgctgagca tcgtccaatc ggcttcgttc aaagcgaaac tgagtcgctg ccattggcct
     6001 atcgccgata gcaaatccga cggccaggcg ttgatctgcg cgctcccgcc cggaccgctc
     6061 aacaacagta cagggtaacc gctcaacaag gtgtcggcgg ggttcgacgg ctcaattttg
     6121 atgtcaaatt ggacaaaacc ccgctcgttc cacagactcc aatcgccatg aaaaaccggc
     6181 ggggcaatgc ccgcagaaac cggccccaaa cgatctgtga tgcgaatgaa attggaattt
     6241 tcgccctcgg caggaaccaa ctgtcgctgc aacatgccgc tgaactgcca gccggcgtat
     6301 ccctcgctca tatcaaaaga ggtacacagt gtggtgtcac ccgaaataat gccggccaga
     6361 gcagtgcttt gacaacatat tgcaaaaaca atggtgggca ggactcgaat catagcgatg
     6421 tgtttttttg ataacagtat cgtttgggaa cagttgcatt ggtcagataa cgaaacaggg
     6481 ctgaatttca tctttgaatc cctacctttg cccgcaaaac cacgctatgc gcacactatc
     6541 ggcaatcata tttttctgga tcggcctcgt tggcgccacc gcccaggacg gcgcccgcaa
     6601 caaaggcaat gccgcgcttt tccacatttc gtacgcattt cagttgcccg ccggcgacct
     6661 ctccgaacgc tacggctcga acaccaacgt gggcattgcc gccgaatgga tgaccgacaa
     6721 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcaatcag
     6781 gtaaaaacag acgtattggc cggactgcgc actgccgaag gtttcatcgt gggcaacgac
     6841 cggggcatcg ccgacatccc gcttaaacaa cgcggctact acatcggagg catggccggc
     6901 aaactgtttc ccatgtccgc caaagaaacg cgctccggcc tccggctcac gctgggcgca
     6961 ggtttgttgc aacataaaat ccgcatacag gacgaccccg aacgcggcgt ggccgccttg
     7021 gccggcgatt ataaaaaagg atacgaccgg ctcagcaacg ggctggcttt caacgctttt
     7081 gcaggctatc aaatgctgag caacgacggg cgcatcaact ttttcatcgg actggaagcc
     7141 tgctacggca tggtgcaaaa ccgccgcagt ttcaactacg acacccgcac gcaagatacc
     7201 cgccgctaca acgatgcgct catcggactg cgcgccggct ggacgctccc tttttacgtc
     7261 ggcaaggctt ccggcgaaat attttattga cccgccatgt tgctgctttg gctatacgat
     7321 gccggcattg ccctgtatgt ggccgccctg cgcatcgctg ctgtatttca ccccaaagcc
     7381 cgcaaatggg tgcaggggcg aaccgcctgg gaagctcgtc tcaaaagtga aattgctgaa
     7441 aaattcagtc ccggcaagcc ccggctctgg atgcactgcg cgtctctggg tgagttcgag
     7501 cagggccgcc cggtgctgga agcctggcga aaagaacact cggacactca gattttgctc
     7561 acttttttct ctccctccgg ctacgaaatc cnnnnnnnnn nnnnnnngaa atccgcaaag
     7621 actaccccgg cgccgacatc gtggcttacc tgcccaccga tacgccccgc gccgcccgtc
     7681 gttttgtgga gttggtacaa cccgattggg tcatttttgt caaatacgag ttttggctgc
     7741 gcactttgtt tgccctcttc cggcgcaaca ttcccgtgct gctcatctct gccgttttcc
     7801 gacccggaca gatttttttc aaagggtacg gcggcctctg gaaaaaggcc ttgtcggaat
     7861 ttcggcacat cttcgtgcag gatgccgctt ccatggaatt gttacaacga catggcattc
     7921 agcggtgtag catcgcagga gatacccgcg tggaccgggt tgtgagcatt gccgaagaag
     7981 gccgggagat agaaatcgtg aagcatttta ccgccggaca taaggtcttc attgccggca
     8041 gcacctggcc acccgatgag gaattgctgg ccgttttgtt gcgggaaaat ctcttgcccg
     8101 gctggaaatt catcatcgcg ccgcaccaga tttcggagtc caatctccgg cgcatggagg
     8161 cgttgctgcc cgcagaatcg gtgcggtttt cagccatcaa ccctgcgcaa ccccttccac
     8221 aggcggtttt actcattgac aacatcggga tgctcaacgc cctgtatcgc tacggcgacc
     8281 tcgcctacat cggcggcgga ttcggcgcgg gcattcacaa cacgctggaa cccatcgctt
     8341 tcggcttgtc ggtaatcttt ggtccgaaat acaaaaaatt cagggaagcc gtagaaatgg
     8401 tacaaaccgg cggcgctgtg tcggtgaaca acgcggagga cttttgccgg gccatcgttc
     8461 attttgccga ggaaaaccat cgcaggcagg cacgccaggc agccttgcaa tatctgcgcg
     8521 acaatgccgg cgctacgcag aagattttag gccggccggg attgtagcat tcacgccggc
     8581 tccaaaaact gcatctcgta cagcctgcgg taagcgccgt cctcgttttg gagtaactca
     8641 tcgtgcgagc cgaactcctc cacctgccct ttgtccaaca ccagcacctt gnnnnnnnnn
     8701 ntttcgtgat gacgaggatg cggtcggctc ggcggatggt gctgaggcgg tgcgcaatga
     8761 tgatggacgt gcgtttggca atcagcgttt cgatggcaaa ctgaatgacc gactccgttt
     8821 cggggtcaat ggaagaagtg gcctcgtcga ggatgagaat gtcgggatcg aaaaccaaag
     8881 cgcgcacaaa ggagatgagt tggcgctgcc ccatgctcag cgtagccccg cgctccatga
     8941 cctgatactc gtagccgccg ggcatttttt caataaactc atgagcgccg atcatttgtg
     9001 ccgcctcgat gacccgttcg cgcgagatgg acgcatcgcg cagggtaata ttttccatca
     9061 ccgtgccgga aaagaggaac acgtcttgca gcaccagcgc aatgcggcgg cggtaggcgt
     9121 gcaactcgta gtcgcggatg tcgtggccgt ccaccttgat atggccctgc tgaatgtcgt
     9181 aaaacctcgt gagcaagctc atggtagtgg ttttgccgga gccggtgctg cccacaatgg
     9241 cgagggtttg gcctgcagag atgtggaaat tgacatcctt cagtacaaaa tcctcgtcgt
     9301 tgtaagcaaa agacacatcc tcgaaagtca gatcacccct gaaacgttcg ggtttcagct
     9361 tgcccgtatt ggcaataaac tccttgcggt cgagcagggc aaacacgcgc tcggaagcca
     9421 ccagccccat ttgcagcgta ttgaactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcggcaatga tttccaccac
     9601 cggaaagaag accgcatagt acagaatggc gtcgagattg gccttggtat attcgcgatt
     9661 gatggcgcga aaacgttggg cttcctgctc ttctgcattg aaaatctgca cgattcgcat
     9721 gcccgtaatg cgttcctgca tgaaggcgtt cattttggaa atctgattgc gcaccacctg
     9781 aaaggaagcc tttacttttt ctttaaaaac ataagccgcc gccaccagaa acggcagcgt
     9841 aatcatacac accaaggtga gtttccaact ggtgtaaaac atgacgccca gcaccgcgat
     9901 gagcgagagc agatcggcga tgatggtgat gatgccttct nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnatggtg cgggtggtag aagtgccgat gggcgtgcgg tcgaagtagc
    10021 tgagccggag gttggtaatg tgttgaaaaa cgcgcacccg cagatcgcgc acaacgccct
    10081 gccccagcca cgaagtaccg tattggaaca catattgcag ccccgcctcc acaatgagca
    10141 acacgatgag gaagatggca atttgagtga gttggcctac tttgtccggg gcgatgtact
    10201 cgtccaccat caccttgatg aggtaaggcc gcagtaccga caggggcgcc agtaggaggg
    10261 ccagcagcgt ggtggcggca aaaagcgcgg cataaggctt agccaatgcc aatacgcgac
    10321 ccagcagggg cattttcctt gtgggcgctg tgttgtctgc catagttttc ggaaagtttt
    10381 ttggggaaac acccgatggc ggcattagtt taatgcggat gttgcgggcg cgacagatgg
    10441 cacgaatgga ggccgcactg ttgaaaagat gtatttcctt tgtaccgtca ggcaataatg
    10501 ccgggcaaaa cacccaaaac caaatttgta atgaacaccc aaaacatcct ttcctggctg
    10561 ctccgcgtgg ccgccgccgc cattttgttg caaaccctgt acttcaaatt caccgcgcac
    10621 ccgcaatcgg tagaactgtt cagcctgctc ggcgtagaac cctggggcag aatcggcacc
    10681 ggaatcattg agctgataac cggcattttg ttgctcatac cgactaccgc atttgtgggg
    10741 gcattcctcg gactgatgag cggcgccatt ttgtctcacc tcgtcttcat cggcatcgaa
    10801 tcgcatggcg acggagggct gttgttcggc ctcgccatag ccgtatttgt gatgtgcgcg
    10861 gccattatgt ggctgcatcg cgggcagggc gcggcgctgt acctatgggt acggaacaag
    10921 tgatggagaa gcttttttga gctgatgttt ttagaaaaaa gtagctttgt ccgacttttt
    10981 gcggcccgta ccggcagtga aaatgacatg ctcacatttc ttaaaaatac tcgttatgag
    11041 cagcttatca caaaatatcc gttgcctggt gttccttttc ctgatggcgg cctgcctgat
    11101 tccggccatg acatcggcgc agcaaaaagt actcatcatc ggcattgacg gctgccggca
    11161 cgacgcgctc cgggccgctt acaccccgca gctcgattcg ctcatcgccg agtctgttta
    11221 cagctacgat gcccttaccg aagaacccac ctggagcggc gtgggatggt cggctatgct
    11281 cacgggcgtg tggcgcaaca aacacggcgt gacggacaac agctttactg gtaaccattt
    11341 tgctgattat ccgcatttta tgaaacgggt agaagcgttc gatcccagtc tgtttacggt
    11401 ttccatcagc cattgggcgc ccatcaatac ccaaattgtg ggcggcggcg gaattgacct
    11461 catcctgagt cctcccaccg attccttggt gagcgttgca gcagtggatg tattgcgcaa
    11521 ccaaaatccg gatgtcattt ttctgcaatt tgacgacgtg gaccacgccg gccacgcaag
    11581 cggtttttcc atgaatgtgc ccgcctacat ggccgccatt gaagccgtgg accgttttgt
    11641 gggctacatc atggctgctt tgaaatcgcg gccgaattat gccggggaag attggctcat
    11701 catcgggagt accgatcacg gcggtatcgg cacttcgcat ggaggggctt cttttgtgga
    11761 acgcaacatc ttttacttcg cctccaaggc aggcagaaca gcaacagaaa tggccaaaac
    11821 tgccgtcctt accaattcca actgcatgcc cgacaccctg gggctgcgca tggcgggcgc
    11881 tacggattat gcaagtgtgc ccaataacgc cttgtttcaa tttggaacct ctcaggattt
    11941 taccatcgag ctccgcatca aaaccgccgg ctggacaggc gatccttctt ttgtcggcaa
    12001 caaaaactgg aacagcggca gcaataaagg tttcgtcatt tcctccccgc tcaacaacca
    12061 aaacatctgg aaagtcaaca tcggcgacgg ccgcaatcgg gccgatgtga acggcagcgc
    12121 cattgccgac aaccgctggc accatctggc cgccgtgttt gaccgcgacg gactgctccg
    12181 cctttttaaa gacggcatgc ccgacggcag caccaatatg gctcatgttt caggcaatat
    12241 caacaacacc ctggccctcg gcatagggca agatggcacc ctcggctacg gatcggccat
    12301 gaacggcatc atcagcgagg tgcgcatctg gaaaaccgcc gtcaacgagg ctaccctggc
    12361 cgcctggcaa tgtacgccca tccatagctc gcatcccaac tacgccgatt tggcaggcta
    12421 ctggcgcatc aacgagggca acggccaaca acttgccgac agcggcccca acggatggca
    12481 cgctttgtac cacggaaacg ctgccgactg ggagccggtg aatctgcccg acacgaccta
    12541 tgatttcagc aacacgcctc gaattgtaga tgtggcaacc accgcactgg cccatctgtg
    12601 cgtgccggta caggaggcct gggaattgga cggccgcgtt gtcggggtag aaattccaca
    12661 gccacacatc agcggcagcc ccctcgcctg cgagcataca caggaaatgt attcattgac
    12721 aaccaacggt ttacaaccca ttcgctggtc ggtaaccaat ggcgtcattt tgagcggcgc
    12781 agactcctct gccgtactcg tgcaatggaa caccgctggc gccggaagtc ttaccgcaga
    12841 gcaatgcaat gtgcaggcgg tatttcccgt cacggtggat gtgtgttcgg caagcagcga
    12901 aatgcagccg ccggctccct attctttgta tcccaatcct acgcagggcc tcttcacagt
    12961 gagcgctgcc gaagcggcct ccatccggcg cgtgggcgta cgcgatgcgc tgggccggga
    13021 gttgcttgtg gcgcagcctg tgtcggggca
//
LOCUS       DAORLF010000067        12939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2579_length_12939_cov_2.155542, whole genome shotgun sequence.
ACCESSION   DAORLF010000067 DAORLF010000000
VERSION     DAORLF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_12939_cov_2.155542"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>343
                     /locus_tag="PK198_07695"
     CDS             <1..>343
                     /locus_tag="PK198_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRF38655.1"
                     /translation="HSQNTSNFYDPKTVQEIKITFAQQNWRYLLDSLRYNGNELLNCA
                     VEVNGTKLKDAGLRYLHLVDLDGARSSRIVNYKTLYNISTHTNLQVDFGGGLKTDDDL
                     SIALENGAFQVT"
     assembly_gap    344..358
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <359..767
                     /locus_tag="PK198_07700"
     CDS             <359..767
                     /locus_tag="PK198_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRF38656.1"
                     /translation="GSIAAENPALFLDWLLACGTDRVILGADSRNRKITARGWTEDSD
                     LDVLDFIQKFENQGVELVTCTDISKDGMLQGPAFDLYGEILDNTHVRLIASGGVTSIE
                     DVKRLCDMGCEGAVIGKAIYEGRITLKELAELC"
     gene            761..>1397
                     /gene="hisF"
                     /locus_tag="PK198_07705"
     CDS             761..>1397
                     /gene="hisF"
                     /locus_tag="PK198_07705"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRF38657.1"
                     /translation="MLKKRIIPCLDIRDGRTVKGVEFVNLRDAGDAVELARQYALQGA
                     DELVFLDITATVENRSTLLHLVEKVAAEINIPFTVGGGVNSVGDVSALIKAGADKVSV
                     NSSAVKNPALLREMAEEFGSQCVVLAIDARRETEGWKVYVHGGRTPTPLLATDWAREA
                     EQLGAGEILLTSMNNDGAKTGFALDITQQIASSVQIPVIASGGAGKVEHFAE"
     assembly_gap    1398..1464
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1514..>1868
                     /gene="hisI"
                     /locus_tag="PK198_07710"
     CDS             1514..>1868
                     /gene="hisI"
                     /locus_tag="PK198_07710"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRF38658.1"
                     /translation="MTLDFEKGDGLVPVVIQHADSLHVLMLGYMNAEALEQTRREGKV
                     TFFSRSKNRLWTKGESSGNFLWVSEIREDCDSDALLVKVRPEGPVCHTGAASCFREES
                     PKGFLYELEQTIAQRI"
     assembly_gap    1869..1962
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2284..2302
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2303..>3058)
                     /gene="proB"
                     /locus_tag="PK198_07715"
     CDS             complement(<2303..>3058)
                     /gene="proB"
                     /locus_tag="PK198_07715"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRF38659.1"
                     /translation="YKGALSERKAAAAVGNPLLLNLYARHFAQYGIPIAQSLCERRHF
                     ADRRQFLQLKETYQELWKNGIIPIANENDVVSNLELKFSDNDELATLMAAGFGAEGLL
                     ISTGTGGLLDAEGKIIPEVKAVDNEILALVRDEKSALGLGGMLSKLTYTRMATRMGIR
                     VAFFTIQTPDGIGLALRGETGTSFLPQKATLNARQKWLGSGSLISGRLVLDAGAVQAI
                     RARKSLLAVGVRSVKGDFDKHDVIELLDGHHHVV"
     assembly_gap    3059..3090
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3091..3289)
                     /locus_tag="PK198_07720"
     CDS             complement(<3091..3289)
                     /locus_tag="PK198_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38660.1"
                     /translation="MNQTLFTFAEKQNDMASLPVLVIKFGTASITRSNGEPDEAVIAA
                     IARQVADLQAQYHIVLVSSGAV"
     gene            3471..4787
                     /locus_tag="PK198_07725"
     CDS             3471..4787
                     /locus_tag="PK198_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF38661.1"
                     /translation="MLNHLFSTDRLRALYPLVQEFLREEIYPYELEWLRMPFSQVEPI
                     LRAKKDKVKALGAWNPYHAESHGGAGLNLMEVAQLSELLGTSPFGHFTFNCQAPDAGN
                     IELLLQYAAADIRRRYLHPLLEGEIRSCFSMTEPDFAGSNPVLMGTTALRDGDEYVIN
                     GRKWFTTSADGAAFAIVMAVTAPNDPNPYRRASMIIVPTDTPGFRLLRNIPVMGHAGD
                     GWHSHGEIEYDNVRVPVSNLLGGEGEGFQLAQERLGPGRIHHCMRWIGICERAFDLMC
                     RRAASRDIAPGMKLGQQQMVQVFIAESRAEIDAARLMVLHAAHSMETRGQKAAALEIS
                     TIKFFAAGVLQRVVDRAVQVHGALGVTDDTLLSYYYREERGARIYDGPDEAHKAGEED
                     FEGVYRVICFAVHLTSSVICCAVYLPLAETTRGQELLPALLTNEFR"
     gene            complement(4802..5431)
                     /locus_tag="PK198_07730"
     CDS             complement(4802..5431)
                     /locus_tag="PK198_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF38662.1"
                     /translation="MKNKFLLMLSAVVGIALFVAAALPLATNTGLKVGDKAPDFQLKN
                     VDGRMVSMADFKDVKGYIVTFTCNTCPYAVMYEDRIIALHNKFAPMGYPVIAIQPNDP
                     EAQPGDAFDKMQARAKEKGFPFVYLFDEGQKVYPQYGATRTPEIYLLDKDLTVRYTGA
                     IDDNAQDAAAVTVKYVENAVAALEKGGNPEPSFVKAVGCSIKVKKKATN"
     gene            complement(5445..5981)
                     /locus_tag="PK198_07735"
     CDS             complement(5445..5981)
                     /locus_tag="PK198_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HRF38663.1"
                     /translation="MRTFLFFAFALFAAFLQAQDSTSTLTAKVLPAMRRVGHIAVYEK
                     YDALAPVLQQNNDTTYVINFWATWCKPCIEEMPYFEQLHQNYANKKVKVILVSLDFSR
                     QLESKLLPFIADRKLQPEVIALTDSKYNEWIDKISPEWSGAIPITIVRKGNERRLVLE
                     EMPDYESLESIVKHFINL"
     gene            complement(5997..>6205)
                     /locus_tag="PK198_07740"
     CDS             complement(5997..>6205)
                     /locus_tag="PK198_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38664.1"
                     /translation="RFTEIHCPTPILIILKNTAGSHEAILSEEQDAEYPPPPNQNPFS
                     LFLFAVQACRQWQFATVCRRLRGE"
     assembly_gap    6206..6219
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6317..7369)
                     /locus_tag="PK198_07745"
     CDS             complement(6317..7369)
                     /locus_tag="PK198_07745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRF38665.1"
                     /translation="MNLKATSISVSSLSQLLLGTAIGDAYGAGVEFQDRKRIRAEVDF
                     SRFFNWRDQIPADEKSRVVFTVNYHAWDYTDDTEMTIGLIRALMSGLPFTEDLLVAEW
                     KAEYERGIRQKGYGRNGHGSMSWYYSGEKTIEEIRGFQRHRSNPGNAPATRATVLGLL
                     PEHLIQEYAAINANATHPHPYAVMSSRCIALAARWMLVLRGIPSEVIRYCVAQVALDE
                     DFAAYLQRVDALGRYESLDHGDWETLCGPQPIVAPWFLPGIEGMPSDSKFTTGCALYV
                     LKHSATAMDALRMSVHTGGDVDSLASITTGIMAGREGLNSLPDWMMEQVEGRAYLENL
                     AEELGEYLCKLWGREN"
     gene            complement(7409..8833)
                     /locus_tag="PK198_07750"
     CDS             complement(7409..8833)
                     /locus_tag="PK198_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRF38666.1"
                     /translation="MTHTEFRQRAHELADWMADYFENVAQYPVKPAVSPGDIKAQLPE
                     HPPIASEPFEALMDDFQKIILPGMTHWSHPQFFAYFPTGNSAPSVLAEMLTATIGAQC
                     MIWQTSPAAEELEERVMEWLRDMLGLPSDWVGVIQDTASTATLAALITAREQATEWAV
                     NQRGFYQSARLRVYASAQAHSSILKDVRIAGYGEDNLVYIPTDDAYAMIPEALEQAIL
                     ADLEQGYLPACAIAAVGTTSSTAIDPLRPIGEICRRHGVFLHVDAAYAGAALLLPEMR
                     GLIDGIEQADSLVFNPHKWMFTNFDCTAYFVRNKSSLLRSFSILPEYLKTPEDQLVNN
                     YRDWGIPLGRRFRALKLWFVIRTYGVEGLQGKIRSHIEYGERLAAVIAAAPAFELMAP
                     VSLNLVCFRYRPQAALSPEDLDRLNEQLLLRLNASGRILLTQTRLSGRYVLRMVAGQT
                     DATWEEMEKGWQLVAEMARGMEVF"
     gene            complement(9310..>10681)
                     /locus_tag="PK198_07755"
     CDS             complement(9310..>10681)
                     /locus_tag="PK198_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38667.1"
                     /translation="SVAILPFKDLSPGGDQEWFGEGIAEEILFALSRVEGLKVAGRSS
                     TFAFSKEETDPREIGRRLNVGAVLEGSIRKVGNRIRITAQLVNTRDGFQIWSESQEQE
                     LSDIFAVQEDIARSVAGKLTGALLGDQSSPLVQRGTGNTEAYQAWLQGRYQLSQRVDG
                     AEQAVGYFQKAIELDPEFAAAHAGLGNAYIWLGYSNSVPSHEAFPQARTHARRALELD
                     PRSAYAWSILGSVHLWYDWDWAAAKSALDTAIAINPAEARAMLDLGWYHALRRDFAAA
                     TEYMLKAVATDSVDLEYNIDLADMYRMWGQYERSAAISEKMRALYPENSDTYWMLGMN
                     DYSRGRYREATAHFEKTVEYSGKEAWAMLHWAMGLAKSGRQEQARRVIREALAAEPLA
                     ENAPVELAMAWLSLGEKDKALGLLESAARSHANWLISISMDPVWDELRQEPRFKKVMQ
                     QMDFPK"
     assembly_gap    10682..10691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10692..11369)
                     /locus_tag="PK198_07760"
     CDS             complement(<10692..11369)
                     /locus_tag="PK198_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF38668.1"
                     /translation="MPPMRRLAAIMFTDIVGYTAMMQRDEADALLRVRRYRELLALHS
                     ERHHGELLHHYGDGSLTIFSSAVEAVICARDLQLDLQKSPATPLRVGIHIGDIERDGD
                     DLYGDGVNIASRVQNLAPVGAVLFTDRVLEDIRSHPELPYKALGKYSLKNIEQPVQVY
                     ALSGPGFADLKSSDLQSPKGKALSGAPQSAAWRIGMGALALGLTALALSAVFLWNQFR
                     PKEQPLAL"
     gene            11461..>12535
                     /locus_tag="PK198_07765"
     CDS             11461..>12535
                     /locus_tag="PK198_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF38669.1"
                     /translation="MQEQQKHGDKHLVRAFGLPAAVILFVSSIIGSGVYKKVAPMSLE
                     LQSPKLVLLAWVLAGVVTLLGVLTTTEIGSMITESGGPYAYFKKIYGKIFAYYYGWSS
                     FSVIQSASIASIAYVFSQSVNAVVELPRLGAAWEEWSILGIFFPFANIGVKVVAVALI
                     VTLVAINYRGVEHGGWVSKVITVLVVSSLALIVLLGIAFGSGSIENVTHAATGFPPAA
                     FTGRFGIVGPMFAAMLAAFWAYEGWLNIGFIGDEVKNPQRTIPLALTIGILIIIGTYL
                     AVNFTYLYVFPIDEMIALAQNTNTIAAVAVMQKLFGDPGLLFISLLIIITTLGCTNAT
                     ILTAARIYFAMARDGFFSQRAARI"
     assembly_gap    12536..12559
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12560..12895
                     /locus_tag="PK198_07770"
     CDS             <12560..12895
                     /locus_tag="PK198_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF38670.1"
                     /translation="ALLMQMVWSCTLVFSGSFDQLTDMLIFASFIFYGSIAFGVFVLR
                     RKMPHAERPYKAFGYPVVPALFVAFCAALVIITIFQRPREAMMGLSLIALGTLPLLYW
                     RRKYGDVKS"
BASE COUNT         2665 a         3786 c         3457 g         2756 t
ORIGIN      
        1 gtcacagtca aaatacgtcc aacttctacg atccaaagac cgttcaggaa atcaaaatca
       61 cctttgccca gcaaaactgg cgctacctgc tggattcgct gcgctacaat ggcaatgaat
      121 tactgaattg cgcggtagaa gtgaacggta cgaaactgaa agacgccggc ctgcgctacc
      181 tgcatctggt ggacttagac ggcgcgcggt cgagccgcat cgtcaattac aaaacgctgt
      241 acaacatctc cacacacacc aaccttcagg tggactttgg tggcggcctc aaaaccgacg
      301 acgacttgtc catcgctctc gaaaacggcg cttttcaggt gacnnnnnnn nnnnnnnncg
      361 gaagcattgc cgccgaaaat ccggctctgt ttttggattg gctcctcgcc tgcggcacag
      421 atcgcgtcat cctcggcgcc gacagccgca accgcaaaat cacagcccgc ggctggaccg
      481 aagactccga cctcgatgtg ttggatttta ttcaaaaatt tgaaaaccag ggagttgaat
      541 tggtcacttg caccgatatt tccaaagacg gcatgttgca aggacctgcc ttcgacctct
      601 acggcgaaat tttagacaac acccatgtgc gccttattgc cagcggcggc gtcacttcca
      661 tagaagacgt aaaacgcctg tgcgacatgg gctgcgaggg agccgtcatc ggcaaggcca
      721 tttacgaggg cagaattacg ctcaaagaat tggccgaatt atgctgaaaa agcgcatcat
      781 tccctgcctc gacatccgcg acggccgcac cgtaaagggc gtggagttcg tcaacctccg
      841 cgatgccggc gacgccgtag aactggcacg gcagtatgcc ttgcagggcg ccgacgagtt
      901 ggttttttta gacatcacgg ccacggtgga aaaccgctcc acgctcctcc atttggtgga
      961 aaaagtggcc gccgaaatca atattccctt cacggtaggc ggcggtgtca actccgtcgg
     1021 cgacgtgtcg gccctcatca aagcgggcgc cgacaaagtg agcgtcaact cctccgctgt
     1081 taaaaatccc gccctcctcc gcgaaatggc cgaggaattc ggcagtcagt gcgtcgtatt
     1141 ggccatagat gcccgccgcg aaaccgaagg ctggaaagtg tatgtacacg gcggcagaac
     1201 gcccacgccg ctgttggcca cagactgggc gcgggaggcc gagcaactcg gcgcgggcga
     1261 aatcctcctc accagcatga acaacgacgg cgctaaaacc ggttttgccc tcgacattac
     1321 gcaacaaatc gcatcctccg tgcagattcc cgtcattgct tcgggcggcg cgggcaaggt
     1381 ggagcatttc gcagaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnncgatac ccgaagtgaa agtttatttg aaaagcaaaa
     1501 acatacccgt aagatgacat tggattttga aaaaggcgac ggtttggtgc cggttgtcat
     1561 tcagcacgcc gattcgctgc acgtcctcat gctgggctac atgaacgccg aagcgttgga
     1621 gcaaacccgc cgcgagggca aggtcacgtt tttcagtcgc agcaaaaacc gcctctggac
     1681 caaaggcgaa agctccggca acttcctgtg ggtgagtgaa atacgcgaag attgcgacag
     1741 cgacgccctg ctcgtcaagg tgcgccccga aggcccggtg tgccataccg gcgcggcatc
     1801 ctgcttccgc gaagagtcgc ccaaaggctt tttgtacgaa ttggaacaaa ccattgcaca
     1861 gcgcattgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcgaagc caaagacaac
     1981 gaccccgacc tcttcaaagg cgaagctgcc gacctgctct atcacctgct cattctgctg
     2041 aaagccaagg gtttttcttt ggaagacatc gaggccgtgc tgcgggcgcg ggcgaagaag
     2101 tgagcatggg ccggcgtagt gaaggggtga ctcgccctgc caaaactgtg agagttaccc
     2161 cttcactaag ccaataagcg cccccctcac aacaccacaa tatcatccgc gtgggcaatc
     2221 tcaaaattct gcgcagccaa gcccgtcggt atatcggctg ccgagtgctt gctgcgcgct
     2281 atgnnnnnnn nnnnnnnnnn nncaccacat ggtgatgccc gtccaacagc tctatcacgt
     2341 cgtgtttgtc aaaatcgccc ttcacgctgc gcacccccac cgccagcagg ctcttgcgcg
     2401 cccggatggc ctgcaccgcg cccgcatcca gcaccaaacg acccgaaata agactgccgc
     2461 tgcccaacca tttttgccgc gcattgagcg tcgctttttg cggcaggaaa ctcgttcccg
     2521 tttccccgcg cagggccaga ccaatgccgt cgggcgtttg tatcgtaaaa aacgccaccc
     2581 gaatgcccat gcgcgtagcc atgcgcgtgt aggtcagttt cgacagcata ccgcctaatc
     2641 ccagcgccga tttttcgtcg cgcaccaatg caagtatttc attatcaacg gcctttacct
     2701 ccgggatgat cttcccttcc gcatcgagca ggccgccagt ccccgtactg atgagcaggc
     2761 cctcagcccc gaaacccgcc gccatgagcg tagccaattc gtcgttgtcc gaaaacttca
     2821 actccagatt gctcactaca tcgttctcgt tggcaatggg aatgatgccg tttttccaca
     2881 attcctgata ggtctctttc agttgcaaaa actgccgccg gtcggcaaag tggcgtcgct
     2941 cgcacaggct ctgcgcgatc ggaatgccat attgtgcaaa atggcgggcg tacaaattca
     3001 acagcagcgg attgcccaca gcggcggcgg ccttgcgctc gctcagcgcg cctttgtann
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaccgctcc cgacgacacc agtacaatat
     3121 ggtattgagc ctgtaaatca gccacttgtc gggctattgc agcgatgacg gcctcgtcgg
     3181 gttcgccgtt gctgcgggtg atggatgccg tcccgaattt gatcacaagt acaggcaatg
     3241 aggccatgtc gttctgtttt tcggcaaagg taaaaagggt ttggttcatg ctctgctgaa
     3301 ttatcagggc ggccacccgc ctcataccga cgttgcatac aaggtaccgg ctgcgggtgc
     3361 cgctcgcctg ccggcaaggc aggggccgtc cactcttacc gccggctccg gcttttgccc
     3421 cctgccgtcg gtaccgtttc cgtccctgct gcaaccaacc gcacaccaat atgctcaatc
     3481 acctcttctc caccgaccgc ctgcgggcac tctatcccct cgttcaggaa ttcctccgcg
     3541 aggaaattta tccctacgaa ttggaatggc ttcgcatgcc cttctcacag gtcgaaccca
     3601 tcctgcgcgc caaaaaagac aaggtcaagg ccctcggcgc ctggaatccc taccacgccg
     3661 aatcgcacgg cggcgcaggc cttaacctca tggaggtggc ccaactcagc gaactgctcg
     3721 gcacctcgcc tttcgggcat ttcaccttca actgccaggc tcccgatgcc ggcaacatcg
     3781 aactgctgct ccagtacgcc gccgccgaca tccgccgccg atacctgcac ccgctgctgg
     3841 agggcgagat ccgttcctgc ttctctatga ccgaacccga ttttgccggc tccaaccccg
     3901 tactcatggg caccaccgcc ctccgcgacg gcgacgaata cgtcatcaac ggccgcaaat
     3961 ggttcaccac ctctgccgac ggggccgcct tcgccatcgt catggccgta accgcgccca
     4021 atgacccgaa tccctaccgc cgggccagca tgattattgt acccaccgac acgccgggct
     4081 tccggctgct gcgcaacatc cccgtcatgg gacacgcagg cgatggttgg cacagccacg
     4141 gcgaaattga atacgacaac gtccgcgtac cggtttccaa cctcctcggc ggcgagggtg
     4201 aggggttcca attggcgcag gaacgcctgg ggcccgggcg tatccaccac tgcatgcgct
     4261 ggatcggcat ctgcgagcgc gccttcgatc tgatgtgccg ccgcgccgct tcccgcgaca
     4321 ttgcgccggg catgaaattg ggccaacaac agatggtgca ggtcttcatc gctgaaagtc
     4381 gcgccgaaat tgacgccgcc cgtctcatgg tgctgcacgc cgcgcacagc atggaaaccc
     4441 gcggtcaaaa agccgctgcc ctggagattt ctaccatcaa gttttttgca gccggggtgt
     4501 tgcagcgcgt ggtggaccgt gcagtgcagg tacacggcgc gctgggcgtc accgacgata
     4561 cgctgctgtc gtattactac cgcgaggaac ggggcgcccg catttacgac gggccggacg
     4621 aggcgcacaa ggctggcgaa gaagattttg aaggggtata tcgggtaatc tgcttcgcag
     4681 ttcatctgac ttcgtcagta atctgctgcg cagtttatct gcctttggcg gaaacgacaa
     4741 gagggcagga attgcttccc gccctcttga caaatgaatt tcgttgacta aacagcaaag
     4801 attagttcgt agctttcttt ttcactttga tggaacaacc aaccgccttg acgaaagaag
     4861 gctccgggtt gccgcctttt tcgagggctg cgactgcgtt ttctacatat tttactgtaa
     4921 cggcagcagc atcttgagca ttgtcgtcaa tggcgccggt gtagcgcacc gtgaggtctt
     4981 tatcgagcag ataaatttcg ggggtgcggg tagcgccata ctgcggatac accttctggc
     5041 cttcatcgaa aagatagaca aacgggaagc ccttttcttt ggcacgcgcc tgcattttgt
     5101 caaacgcatc gccgggctgc gcttccgggt cattgggctg tatggcgatg accgggtagc
     5161 ccatcggagc aaatttgttg tgcaatgcaa tgatgcggtc ttcgtacatc acggcgtagg
     5221 ggcaggtatt gcaggtaaaa gtgacgatgt agcccttcac gtctttaaaa tcggccatgg
     5281 aaaccatgcg gccatccacg tttttgagct gaaaatcagg cgctttgtcg cctactttca
     5341 gtccggtgtt cgtggcaagc ggcaaggcgg cggccacaaa caaagcaatg ccgacaacgg
     5401 cggagagcat gagtaagaac ttgttcttca tgttgataac agatttacag gttgataaaa
     5461 tgtttgacga ttgattccaa agattcgtag tcgggcattt cctccagcac caaccggcgt
     5521 tcgttgcctt tgcgcacaat ggtgatggga atggcgccgc tccattcggg gcttattttg
     5581 tcaatccact cgttgtattt gctgtcggtg agggcaatga cctccggttg cagtttccgg
     5641 tcggcaataa agggcaacaa cttggactcc aactgccgcg aaaaatcgag gctgacgagg
     5701 atgactttta cctttttgtt ggcgtagttc tggtgtaact gctcaaaata aggcatttct
     5761 tcgatgcagg gtttgcacca ggtggcccag aaattgatga cgtaagtggt atcgttgttt
     5821 tgttgaagca ccggcgccag cgcgtcgtat ttttcataca cggcaatgtg gcccacgcgg
     5881 cgcatggccg gcagcacttt ggccgtgagc gtggaggtgc tgtcttgcgc ctgaaggaag
     5941 gcggcaaaaa gagcaaacgc gaaaaaaagg aaagttctca tagtcggatg tttctattat
     6001 tcgcccctta aacgacggca aacggtagca aattgccatt gtcggcacgc ctgcaccgcg
     6061 aataaaaaca gcgaaaacgg gttttggttg ggcgggggtg ggtactcagc gtcttgttcc
     6121 tccgatagga ttgcctcgtg gcttcctgcc gtatttttta aaatgatgag gattggggtt
     6181 ggacaatgaa tttcagtgaa tctccnnnnn nnnnnnnnna attaaagagt aatcgtgtcg
     6241 gccccccagc cattggggtt gaagccggtt tcaaacaaat agcatggggt atgatctaaa
     6301 tgacgaccgg tttttctcaa ttctctcggc cccacaactt acacagatac tcccccaatt
     6361 cctccgccag gttttccaaa tacgcccggc cctccacctg ctccatcatc caatccggca
     6421 agctgttcaa accctcccgt ccagccatga tgccggtggt aatggatgcc agcgagtcca
     6481 cgtctccgcc ggtatgcacc gacatacgga gcgcatccat agccgtagcg ctgtgtttga
     6541 gaacgtacag ggcgcatccg gtggtgaatt ttgaatcaga aggcatccct tcaatgccgg
     6601 gtagaaacca gggcgccaca atgggttgcg ggccacaaag cgtttcccaa tctccatgat
     6661 ccaaactttc ataacggccc agcgcatcca ctcgttgaag ataggcggca aaatcctcgt
     6721 ccaacgctac ttgagccacg caataccgaa tcacctctga gggaatccct cgcaaaacca
     6781 acatccaccg ggcggccaga gcgatgcacc ggctcgacat caccgcgtac gggtgcgggt
     6841 gggtggcatt ggcattgatg gcggcgtact cctgtatgag gtgttcgggc agcaaaccga
     6901 gcacagtggc tctcgtggcg ggagcgttgc cggggttgct ccgatggcgt tggaaacctc
     6961 ggatttcttc tatcgttttt tcgccggagt agtaccagct catcgaaccg tggccgtttc
     7021 tgccgtatcc tttttggcga atgccgcgct cgtactcggc tttccattcc gctaccaaca
     7081 agtcttctgt aaacggcagt cccgacatga gcgcccgaat gaggcctatg gtcatctccg
     7141 tatcgtcggt gtaatcccag gcgtgataat tgacggtaaa tacaacccgc gacttttcgt
     7201 cggcgggaat ctgatcacgc cagttgaaaa agcgagaaaa gtccacctct gcacggatgc
     7261 gcttccggtc ctgaaattcc actcccgcgc catacgcatc accgatggcc gtaccgagaa
     7321 ggagttggct gagcgaggaa accgagatgc tggtagcctt gagattcatg gtgaaaagga
     7381 ttaaaggatt ttcatacccc ccgaactact aaaaaacctc cattccccgc gccatttccg
     7441 ccaccaattg ccagcccttc tccatctcct cccaggtagc gtctgtctga ccggccacca
     7501 tgcgcaggac gtagcggccc gacaggcggg tttgcgtcag caggatgcgg cccgaagcgt
     7561 tgaggcgcag caggagttgc tcgttgaggc ggtctaaatc ttcgggactg agggctgcct
     7621 gcgggcggta ccggaagcaa acgaggttga gcgatacggg cgccatgagt tcaaaagcgg
     7681 gcgcagcggc aatgacggcg gccaagcgct ccccgtactc gatgtgcgaa cggattttgc
     7741 cctgaagccc ctctacgcca taagtgcgaa tgacgaacca gagcttgagg gcgcgaaacc
     7801 ggcggcccag cggtatgccc cagtcgcggt agttgttcac gagctggtct tcgggggttt
     7861 tcaggtattc cggcaggatg ctgaaactgc gcagaagcga gcttttgttg cgcacaaaat
     7921 aggccgtgca gtcgaaattg gtgaacatcc atttgtgggg attgaacacc aaactgtcgg
     7981 cctgctcaat gccgtcaatg aggccgcgca tttcgggcag cagcagagcg gccccggcgt
     8041 atgcggcgtc cacatgcagg aaaaccccgt ggcggcggca gatttcgccg atgggccgca
     8101 gcgggtcaat ggcagtagaa ctcgtggtgc ccacggcggc tattgcgcag gcgggcaggt
     8161 agccttgttc cagatcggct aagatggcct gctccagcgc ttcgggaatc atggcgtagg
     8221 catcgtcagt gggaatgtaa actaagttgt cttcgccgta gccggcaatg cgcacgtctt
     8281 tgaggatgga cgaatgtgcc tgcgccgagg catacacccg caggcgggcc gactgataaa
     8341 acccgcgctg gttgacggcc cattcggtgg cctgttcgcg ggcggtgatg agcgcggcga
     8401 gggtggcggt agaggccgtg tcctgtatga cgcccaccca gtcggaaggc aggcccagca
     8461 tatcgcgcag ccattccatc acgcgctctt cgagttcttc ggcggcgggc gaggtctgcc
     8521 agatcatgca ctgcgccccg atggtggcgg tcaacatttc ggccagcacg gagggcgcgc
     8581 tgtttccggt ggggaagtag gcgaaaaact gcggatggct ccaatgtgtc attcccggca
     8641 ggatgatttt ttgaaaatcg tccatcagcg cctcgaacgg ctccgaagca atcggcgggt
     8701 gttcaggcag ttgcgccttg atgtcgccgg gtgatacggc gggtttcacc ggatattggg
     8761 ccacgttttc gaagtagtcg gccatccagt cggcaagttc gtgggcgcgt tggcggaatt
     8821 cggtatgtgt cattggatgc ttgtgtttat tgaatactta ccaatagctg ctggccgtta
     8881 gctgctggct gttagcttgt ttattattag gaaattcgat tttccattgg ggaaaatttc
     8941 aaaactccaa ttttgaaaac gagtccctta tttttagtaa ctacttagtt tagcttattt
     9001 ttcaaagcta caagctgctg ccacgccccg gcgtggtgtt agcttatttt ttctttagga
     9061 agaagatttt cctcatggga aatttctaaa cgtacttttg aaaatgaact cttctccttt
     9121 tggaaaatca accacgcagg gcgtggttgc ggctaatagc taacggccaa tgctaaataa
     9181 atgctatttt tattaaaaaa aagccccgcg accagcggtt tgcagcttgc agccgcccac
     9241 tgcctaccgc cctctgaaca ctctacgcag gtaccgccga ccgcccaccg cataccgcta
     9301 accggcaact cacttcggaa aatccatctg ttgcatcact tttttgaacc tcggttcctg
     9361 acgcaattca tcccatacag ggtccatact gatggaaatg agccagttgg cgtgcgagcg
     9421 ggcagcggat tccagcagac ccagcgcttt gtctttttcg cccagactca gccaggccat
     9481 agccagttcc accggcgcat tttcggccag cggctcggcg gccaatgcct cccgaatgac
     9541 gcgacgggct tgttcctgac ggccagattt ggccaatccc attgcccagt gcagcatggc
     9601 ccaggcttcc ttgccggagt actccaccgt tttttcaaaa tgagccgtcg cctcccggta
     9661 cctgccccgc gagtagtcgt tcatccccag catccagtag gtatcgctgt tttcgggata
     9721 gagcgcgcgc attttttccg agatggcggc gctgcgctcg tattggcccc acatgcggta
     9781 catgtcggct aagtctatgt tgtattccaa atccacgctg tcggtggcca ccgccttgag
     9841 catgtactcg gtagcggcgg caaaatcgcg gcgcaaggcg tggtaccagc ccagatcgag
     9901 catggccctg gcttcggcgg gattgatggc aatagcggtg tccaatgcgc ttttggcggc
     9961 agcccaatcc cagtcgtacc acaggtgtac cgatcccaaa atgctccagg cataagccga
    10021 acgcggatcg agttcgaggg cgcggcgggc atgggtgcgg gcctgcggaa atgcctcgtg
    10081 cgagggcacg gaattgctgt atcccaacca gatataggca ttgcccaaac cggcatgagc
    10141 ggctgcaaat tcggggtcca actcaatggc tttttggaaa tatcccaccg cctgctcggc
    10201 gccgtccacc cgctgactga gctggtaacg cccctgcaac caggcctgat acgcttcggt
    10261 attgccggtg ccgcgctgca ccaagggcga ggactgatcg cctaaaagcg ccccggtgag
    10321 tttgccggct accgagcggg caatgtcttc ctgtacggca aaaatatcgc tcagctcctg
    10381 ctcctgcgat tccgaccaga tttggaaacc gtcgcgggtg ttcaccaatt gcgccgtgat
    10441 gcggatgcgg ttgcccacct tgcggatgct gccttccagc accgcgccca cattgagccg
    10501 ccgcccgatt tcgcgggggt cggtttcttc cttgctgaac gcgaacgtgg agctgcggcc
    10561 cgccactttc agcccctcta ccctactgag ggcgaagaga atttcttcgg caatgccctc
    10621 gccgaaccat tcctgatcgc cgcccggact gaggtctttg aacggcagga tggccaccga
    10681 gnnnnnnnnn ncaatgccaa aggctgttct ttgggtcgga actgattcca caaaaacacc
    10741 gccgacaggg ccagagccgt cagtcccaat gccagcgcgc ccatgccgat gcgccaagcg
    10801 gcagattgcg gagcgcccga caacgccttg cctttcgggc tttgcaggtc gctgcttttc
    10861 agatcggcga aacccggccc gctgagggca tatacctgca ccggctgctc tatgtttttc
    10921 agggagtact tgcctaatgc cttgtacggc agttccgggt ggctgcggat gtcttccagc
    10981 acgcggtcgg tgaagagcac ggcgcccacc ggagctaagt tttgcaccct ggaggcaatg
    11041 ttcacgccat cgccgtagag gtcgtcgccg tcgcgctcaa tgtcgccgat gtggatgccc
    11101 acgcggaggg gcgtggcggg cgatttttgg aggtccaatt gcagatcgcg ggcacaaata
    11161 acggcctcta ccgcgctgga aaaaatggtg agactgccgt cgccgtagtg gtgcagcaac
    11221 tccccgtgat gccgctccga atgcaaggcc agcaactccc ggtagcggcg cacccgcaac
    11281 agcgcatcgg cttcgtcgcg ttgcatcatc gcagtgtagc ctacgatatc ggtaaacatg
    11341 atggctgcca atcggcgcat gggcggcata gagcgatggt tgtacggggc caaaaataca
    11401 atgaatcggt tgtctgtatc atgtatttct ctacttttgt tgaatacatc aaaattgacc
    11461 atgcaagaac aacaaaaaca cggagacaaa cacttggtca gagccttcgg cctgcccgct
    11521 gccgtcattc tttttgtcag ctccatcatc ggatcgggcg tttacaaaaa agtggcgccc
    11581 atgtcgctcg aactgcaatc gcccaagctg gtactgctgg cctgggtgct ggcgggcgtc
    11641 gtcacgctgc tgggcgtgct caccaccacc gaaatcggct ccatgatcac cgagagcggc
    11701 ggcccgtatg cgtatttcaa aaaaatatac ggcaaaattt tcgcctacta ctacggctgg
    11761 agcagctttt ccgtcataca gtccgcctcc attgcgtcca tcgcgtatgt attttcgcag
    11821 tcggtcaacg ccgttgtcga actgccccgc ctcggcgccg cttgggagga gtggtccatt
    11881 ctgggcatct ttttcccttt tgccaacatc ggcgtcaaag tagtagccgt agcgctcatt
    11941 gtcacgctgg tagccatcaa ttaccgcggc gtcgagcacg gcggctgggt cagcaaagtc
    12001 atcaccgtgc tggtggtttc cagcctcgcg ctcatcgtat tgctcggcat cgccttcggc
    12061 agcggcagca tcgaaaacgt cacccacgcc gctaccggct tcccgcccgc cgccttcacc
    12121 gggcgcttcg gcatcgtcgg ccccatgttt gcagccatgc tggccgcctt ctgggcctac
    12181 gagggctggc tcaacatcgg cttcatcggc gacgaggtca aaaacccgca acgcaccatt
    12241 ccattggccc tcaccatcgg catcctcatc atcatcggca cctatctggc cgtcaatttc
    12301 acctacctct acgtcttccc cattgacgaa atgatcgcgc tggcccaaaa caccaacacc
    12361 attgccgccg tagccgtcat gcaaaaactc ttcggcgatc cgggcctgct tttcatctcg
    12421 ctgctcatca tcatcaccac cctgggctgt accaacgcca ccatcctcac cgccgcccgc
    12481 atctatttcg ccatggcccg cgacggcttc ttctcgcagc gcgccgcccg tatccnnnnn
    12541 nnnnnnnnnn nnnnnnnnng ccttgctcat gcagatggtg tggtcctgta ccctggtctt
    12601 ctccggctcc ttcgaccaac tcaccgacat gctcatcttc gcgtccttca ttttctacgg
    12661 ctccatcgcc ttcggggtgt tcgtgctccg ccgcaaaatg ccccacgccg agcgcccgta
    12721 caaagcattc ggctacccgg tcgtgccggc cctttttgtc gcgttttgcg ccgcattggt
    12781 catcatcacc attttccagc gcccccgcga agccatgatg ggcctctcgc tcatcgcatt
    12841 gggtaccctg cccctgctgt attggcggag gaagtacggc gatgtaaaaa gctgattttt
    12901 ggggggcgcc accccgcact gccagcctcc cccagcccc
//
LOCUS       DAORLF010000068        12933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2580_length_12933_cov_1.558161, whole genome shotgun sequence.
ACCESSION   DAORLF010000068 DAORLF010000000
VERSION     DAORLF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2580_length_12933_cov_1.558161"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PK198_07775"
     CDS             complement(<1..422)
                     /locus_tag="PK198_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6371 domain-containing protein"
                     /protein_id="HRF38671.1"
                     /translation="MSDSNDRRPVAVVESAKTCLIMTGFEPSAVWLAVGSLSYLNAER
                     LAPAKNRTIILYPDKGCFQAWELKASELRAAGFVVAVSDFLERHTDARAKDDIADLYI
                     RATLAPNAPQSAQERPTASEPPQHTTEPPQPDTAPGNGL"
     gene            complement(476..>883)
                     /locus_tag="PK198_07780"
     CDS             complement(476..>883)
                     /locus_tag="PK198_07780"
                     /inference="COORDINATES: protein motif:HMM:NF039741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6371 domain-containing protein"
                     /protein_id="HRF38672.1"
                     /translation="PAALQSVQHTQAQVKPESARRPIEIPAEYFQPSLKGYGANNFVK
                     FLCRLFGEARALELAALYHLGTSNAIWPGACIFWLVDRTGRTFAGQVFDFDPATGKTI
                     RETGADGRERRRSNWVHYAIDRSLKAQGRPPPG"
     assembly_gap    884..981
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1429..>2122)
                     /locus_tag="PK198_07785"
     CDS             complement(1429..>2122)
                     /locus_tag="PK198_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HRF38673.1"
                     /translation="VLANYFTEHIDVNDKDGLFCLAENLIINLDELAQLSKFEINKIK
                     AILTRHVVKERPPYGTKKIPFPRRATFFASTNDDEFLTDTTGNVRWLVFEVDEILHDN
                     GGPNGYSQQVDINLVWAQALALLRSGYNYILSPDEIAKSERNNRTHQLLSIEAALIQE
                     CLAPANKSDASAQFMTAAQILTYLENETNTKQKLFHNNIGKAMSFLGFTKDQGYIREK
                     GFQVKGYYVQKI"
     assembly_gap    2123..2270
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2271..>2478)
                     /locus_tag="PK198_07790"
     CDS             complement(<2271..>2478)
                     /locus_tag="PK198_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38674.1"
                     /translation="EWRELNSDNLLIELYEVGFNGIEKMLSALLRSDFVEKFNPFKAF
                     FESLPEWKPGDPDYIEKMAGFVRA"
     assembly_gap    2479..2709
                     /estimated_length=231
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2979..3005
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3006..3199)
                     /locus_tag="PK198_07795"
     CDS             complement(<3006..3199)
                     /locus_tag="PK198_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38675.1"
                     /translation="MRANELILLSRDQVEEIVIDAVKTVLSLHAKENAVPGPAMPVVM
                     TLEQVCRYTGLSKDAIYRKTG"
     gene            complement(3294..>3688)
                     /locus_tag="PK198_07800"
     CDS             complement(3294..>3688)
                     /locus_tag="PK198_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38676.1"
                     /translation="AAELLKFNAAFTRRRSNVGRGKAAQERTEGPTPAHFGPEFMKIV
                     SEALHKMELLNDAGQYIGPLKEKAELAALYRALYRRNYLRRGLSWADAFGKWLGDTFG
                     ASVSGKTLEKLPTPEGEAIEKDFLYYID"
     assembly_gap    3689..3728
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3729..>4063)
                     /locus_tag="PK198_07805"
     CDS             complement(<3729..>4063)
                     /locus_tag="PK198_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38677.1"
                     /translation="SIEIITGLLFSMPARQEDQQRRESLEQVRADGKRLIAICCERCR
                     ATLLQVLEIGATETEFFALVDFFRGHSYTSFGAPRHKGRPDIPAIVSEVFALADALPD
                     ALRAYFLEA"
     assembly_gap    4064..4172
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4173..4462)
                     /locus_tag="PK198_07810"
     CDS             complement(<4173..4462)
                     /locus_tag="PK198_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38678.1"
                     /translation="MKNYNIKTIQFDEEIGIGAACQADNNLLHFVLSCVAKMAAGEVG
                     DVSEADAFRWSGIPIPDYHETEHVTSSWKLPAPFFTFYNERVDFVFIFLYRNT"
     gene            complement(4499..5899)
                     /locus_tag="PK198_07815"
     CDS             complement(4499..5899)
                     /locus_tag="PK198_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38679.1"
                     /translation="MRFRLTLPQNEAFFQRYAGLVPTLSKLSYVAQVVSALTEAGILY
                     ALIRNAVADIAPPMLAGVVGIAGAQLATAFIEIGLRKFLPFAARQILNKRFAGLDLVM
                     SIAIFAASLLLLTASGYLSFAGSKETVAAVVPPPKTEGTGAIDTTVNSHRESNLTSYR
                     RDSAAIAGRYASQIAALEMAHKAATDANNTKLKGIESRERNTGQSFATQKATTKQTIA
                     DATAAHALKLAALTEAGAKEMAALQDEHSALNADLNRERSEWLAALKERNAQAEAKRQ
                     KQVSAWGGSVAWFTVLCLAVLLLAIALQEVHAHGSEIQEEALPTEYFFRQSPVAAFMA
                     ALSERFHAKIFALIKRIEDGTPEPPEPGKPPILYQYQQKAERREIGFTANRANQQPPP
                     PTNAPNGSNALRYNASGYRVADCPQCGQTFEQRTTWQKYCCEACRIAYHAGQHAGQPF
                     DPGKQRKGRKAKTGKQ"
     assembly_gap    6017..6149
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6488..6514
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6515..7169)
                     /locus_tag="PK198_07820"
     CDS             complement(<6515..7169)
                     /locus_tag="PK198_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HRF38680.1"
                     /translation="MEQRANKKNQKTRGTWWSTLKILRQYWPNDVALAAIEKRDVIGF
                     RDHLAALKGLADSSKSLYLTALKTTLKDAYKLDYLSKDFTAYIEPIKSNPAQKEYLTL
                     EELEHLAVTPCERLDLRNAFLFSAMSGLRYSDIFKLMWGEVQGDEETGFALRYRQQKT
                     QKNETLPISKGARALLGPAGLPNDKVFPTLPEKIFANNMILLQRWFLAAGISKKITFH
                     "
     gene            7227..7571
                     /locus_tag="PK198_07825"
     CDS             7227..7571
                     /locus_tag="PK198_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38681.1"
                     /translation="MYVQLTLGADVVGQSAGFFIVAGFVWGFGFVVSEKPQELTAGFF
                     SRRGVKYGRPKIQKKTLPAFCQRLAPKMNAGRLAIAHICTLFDFRYKYKAIVSIIGTK
                     KPKKLNGHSSGN"
     gene            complement(7705..8535)
                     /locus_tag="PK198_07830"
     CDS             complement(7705..8535)
                     /locus_tag="PK198_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRF38682.1"
                     /translation="MNQNSNRQALRDRLFTIIFEADTPAGKIFDVSLLVAILLSVLVV
                     MLESVKTFPDEVHHLFVVLEWVFTIFFTIEYLLRLYTVGNRLKYAASFFGIIDLVSIL
                     PTYLSFILGGAQSLMVIRILRLLRVFRIFKLTGLMDHGNHIAGALKASRYKISIFLYV
                     VVLVTFVFGSVMYFVEGGVNESFDSIPRSVYWCIVTITTVGYGDISPVTPLGQFLASV
                     LMIIGYSIIAVPTGIITSEIMRKPPEKITTQVCPHCLSEGHDADAVYCKFCGGHLGNH
                     "
     gene            complement(8545..9111)
                     /locus_tag="PK198_07835"
     CDS             complement(8545..9111)
                     /locus_tag="PK198_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38683.1"
                     /translation="MNVLKFNCLDKMENLEKLLGVGSRVRHPAYGDGVVIRLHKAAYE
                     LSFMLYGIKHVGKEYDKWEIIEALPAEEAVTFTEAERSLLKILRNYNAVQEEVELGDR
                     WEGGMMILKPADNTLKEKEIPIESFFHKIVMTRDRLRVMEQRINASKLTDEDKVNLQQ
                     YITKIYGSLTTFNVLFKDKEDYFVGEKS"
     gene            complement(9170..10591)
                     /locus_tag="PK198_07840"
     CDS             complement(9170..10591)
                     /locus_tag="PK198_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRF38684.1"
                     /translation="MKKRNIPLHWRIIMGLIAGIIYSFVSIHFGWNKFTLDWIDPFGV
                     IFIRLLKFIAVPLVLFSIISGVSTLPDPAKLGRMGAKTLGMYLFTTITAVGLGLLLAN
                     LFQPGEFIAKEQRIKNRIKYEIWAEQTPGVGVLDGQNYRAKPEYAAFAGEAAIQMEKE
                     QGNETVESRKAAAESFKNAGPLQFFVDMVPDNIFASLSDSKSMLQVIFFALFFGIVTM
                     FLPKESMKPVLDVINGANEVFLKMVEWIMEAAPFFVFALMSGVLAKMAKTPAEAVEIF
                     KGLLTYSLTLLAGLALLVAVFYPGLILLLVRKISYSSFFKRISPAQFLAFSTSSSAAT
                     LPVSMECVRDNLGVSKNVTNFVLPIGATVNMDGTALYQAVAVVFLAQLHLIDLTLSQQ
                     LTIVLTATLASIGSAAVPSAGLIMMIIVLQSVGLNPAWIAIIFPVDRILDMCRTVANV
                     TSDITVATIIAHSEGELEYREQG"
     gene            10741..>11018
                     /locus_tag="PK198_07845"
     CDS             10741..>11018
                     /locus_tag="PK198_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HRF38685.1"
                     /translation="MGWFKRLKEGIQTATRNKKEAPEGLWYQCKECRATSTRRELKDN
                     FSKCPSCNYHVRISSQEYFEILFDGQADELFGDLRSKDMLNFVDLKPYS"
     assembly_gap    11019..11134
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11135..11646
                     /locus_tag="PK198_07850"
     CDS             <11135..11646
                     /locus_tag="PK198_07850"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HRF38686.1"
                     /translation="NFIGGSMGSVMGEKISRAVDHCLEHKTPLLIISKSGGARMMESA
                     FSLMQMAKSSAKLSLLVKAGIPYFSLMTDPTTGGVTASFAMLGDINFAEPKALIGFAG
                     PRVIKETIKRELPEEFQTSESLLENGFLDFIVDRQDLKDRMADLLAFFDAAPVANGSA
                     DAALEASAE"
     assembly_gap    11691..11768
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11932..12669)
                     /locus_tag="PK198_07855"
     CDS             complement(11932..12669)
                     /locus_tag="PK198_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF38687.1"
                     /translation="MLSRLSYLYRAWRYRFRVDPAEIHYLLSTLRPGDTAVDIGCHKG
                     GYLYWMRRRVGLHGRVYAFEPQPRLFEYLTVIQKQLNFNNVQLENMGLSSQTGSMTLH
                     IPASSSGTSPGATLNAVTESNPTDFHHHTVQVSTLDAYFHAQGIRPALLKIDVEGHEW
                     ELLKGGQALLEACRPRILMECENRHLGGGDVQDVFRFLLDLGYAGRFIHKGKLRPLAE
                     FDAAIHQHQGPGRFWEAPDYVNNFVFE"
BASE COUNT         2797 a         2946 c         3103 g         3080 t
ORIGIN      
        1 aggccgtttc ccggcgctgt gtcgggctgt ggtggctcgg tggtgtgttg gggcggctcg
       61 gaggctgtgg ggcgctcctg ggcggattgt ggggcgttgg gcgcaagggt ggcacggatg
      121 tataaatcgg caatgtcgtc ttttgcgcgg gcgtctgtgt gcctctcaag aaagtcggaa
      181 acagcaacga caaagccggc ggctcgcagt tcagaggctt tcaactccca agcctggaaa
      241 catcctttgt cagggtaaag aatgattgtt cgatttttgg ccggcgccaa ccgttcggca
      301 tttaagtagc tcaatgaacc aacggccagc catacggcgg acggctcaaa gcccgtcatt
      361 atcaaacagg ttttggcgga ttccacaacg gcaacgggcc gccggtcgtt gctgtcgctc
      421 aattgatgca agccaaagca gaacgggaaa cgggcggctt gctcgatgta ggtttttagc
      481 caggcggggg ccggccctgg gctttcaaac tccgatcaat ggcgtaatgt acccaattgg
      541 aacggcggcg ctctctccca tcggcgccgg tttcccttat cgttttgccg gtggccgggt
      601 caaaatcaaa aacttgccct gcaaaggtgc ggcccgttcg atctaccaac caaaagatgc
      661 aagcccctgg ccaaatggcg ttggacgttc ccaagtgata aagcgcggcc aattccaggg
      721 cgcgggcttc gccgaacaaa cgacaaagga attttacaaa gttattggcg ccgtacccct
      781 tcaaactggg ttggaaatat tccgccggta tttcgatggg ccggcgggcc gattctggct
      841 ttacttgcgc ttgagtatgt tgaacagatt gaagcgcggc gggnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn ngaataggcc ggtttggcgg tcaatatagc gggaaaattt
     1021 tccggccttt ttgcattgcg ggcaagtgtg gcgggtttta tggccccggt aaggctccaa
     1081 gcgataccgg cctatgttgt atgctttgtt tgacattttt caaaagtttt aaaaaatgcg
     1141 ttttttacct acttacttac ttattagcat ctaatatact gaatatcaat ttgaaagact
     1201 aagtaagtag gtattgatct aagtaagtac ccttacttac ttagcctaca ttttcgtctt
     1261 ttttgccttt tccggccttt gccgtctcta cggaaactgt aaatgttcaa gctaatttac
     1321 taagtaacta agtaaggact aagtaagtac atttcttacc tacttactta gtctaaaatt
     1381 ctgatttaca aactattaaa ttaaactaag taactaagta agtagaaatt agattttttg
     1441 aacgtaatac ccttttacct gaaagccttt ttctcttatg tagccctggt ctttggtaaa
     1501 acctaaaaaa ctcattgctt tcccaatgtt gttatgaaag agcttttgtt tagtgttggt
     1561 ttcgttttca aggtaggtta gtatttgcgc ggcggtcata aactgagcgc tcgcgtcgct
     1621 cttattggcg ggggctaaac attcttgaat aagggcggcc tctatgctca aaagttgatg
     1681 cgttcgatta ttccgttcgg actttgcaat ctcatcaggt gacaaaatgt aattgtatcc
     1741 actacgcaac agtgccagcg cttgcgccca caccaaattt atgtcaacct gttgcgagta
     1801 accgtttggc ccgccgttgt cgtgcaaaat ttcgtcaact tcaaacacca accaacgaac
     1861 gtttccggta gtgtctgtta aaaattcgtc gtcgttggta ctcgcaaaaa atgtggctcg
     1921 gcgtggaaat ggtatctttt ttgtgccgta tggcgggcgc tcttttacaa cgtggcgggt
     1981 aagtattgcc tttattttat tgatctcaaa tttgctcagt tgcgccaatt cgtcaaggtt
     2041 aataattagg ttctccgcca ggcaaaaaag cccgtcttta tcatttacat ctatatgttc
     2101 tgtaaagtaa ttggccaaga ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttggcacgca
     2281 caaaaccggc cattttttca atgtaatcag ggtcgcctgg cttccattca ggcaatgatt
     2341 caaagaaggc tttaaacggg ttaaattttt caacaaaatc actccttaat aaggcagaca
     2401 acattttttc tattccgtta aaaccaacct catacaattc aattaaaagg ttgtcgctgt
     2461 ttagctcccg ccattctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnt tttcgcgttc tttgcaatgt catcagccaa agatattttt ggcacattgc
     2761 cttgactatc attgagtttc ataaatgtaa ttgtttttgc gggggcgctc acatgggcgc
     2821 ccccgctttt taaaaacaga aactaacgac acacattatt tgcggtaagc ggccgcccgt
     2881 tctcggatgc gggccgggtc aacagagttt cccaacaacc aggcgtcaat tttgccccgg
     2941 tcgaaatata agcgcttgcc ccgtttgctg tgcggaatnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnccggt ctttcgataa atggcgtctt tgctaagtcc agtatatcgg caaacttgct
     3061 caagggtcat cacaacaggc attgccgggc cgggtacggc attctcttta gcgtgcaggc
     3121 tcaagaccgt tttcacggcg tcaattacaa tttcttctac ctggtcacgg cttaacaaaa
     3181 tcagttcatt tgctcgcata ctttattgtt ttttgatgct gcaaatctca gagaatgacg
     3241 tttccgacgt ttccgacgtt tccagaaaat tacggaaacg tcgtttttgc ctgttaatca
     3301 atgtaataca ggaagtcttt ttctattgct tccccttcag gcgttggcaa tttctccaac
     3361 gttttaccgc tcacacttgc cccaaatgta tcgcccaacc acttaccaaa ggcatcggcc
     3421 caagacaaac cccggcgcaa gtagttgcgg cgatacaatg cgcggtaaag tgcggccagt
     3481 tctgcctttt cctttagcgg cccgatgtat tggccggcat cattgagcag ttccattttg
     3541 tgcaacgcct cggaaacgat cttcataaac tccggcccaa agtgcgccgg cgttggtcct
     3601 tctgtgcgct cctgggcggc cttgcccctt ccaacgttgg accgtcggcg ggtaaatgct
     3661 gcgttgaact tgagaagttc ggcggctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnncg gcctctaaga agtaggcgcg caaggcatcg ggcaaggcat cggccagcgc
     3781 gaacacctcc gaaacaatgg ccggaatatc ggggcggcct ttgtgcctgg gcgctccaaa
     3841 acttgtatag ctgtggcccc ggaaaaagtc aaccagggcg aaaaactccg tttcggtggc
     3901 gccgatctca aggacttgca acaaagtggc gcggcatcgt tcgcaacaaa tagcaataag
     3961 ccgtttacca tcggcccgca cttgttccaa tgattcgcgg cgctgttgat cctcttgcct
     4021 tgcgggcatg gaaaacagca ggcccgttat tatctcgatg gatnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtattcct gtaaagaaat ataaacacaa
     4201 aatctaccct ttcattatag aaagtgaaga agggcgccgg cagcttccaa gatgatgtta
     4261 catgttcggt ttcgtggtaa tcgggtattg gtatgcccga ccatcgaaac gcgtcggctt
     4321 cgcttacgtc gcccacttcg ccggcggcca ttttggcaac gcaactcaag acaaaatgaa
     4381 gtaagttatt atcggcctgg caagcggcgc caatgccgat ttcctcgtca aattggatgg
     4441 ttttgatatt gtaatttttc atcttaattg aattttgaag tgaagatgtg gcagcgcttt
     4501 attgcttgcc tgtttttgct ttgcgcccct tgcgctgttt gcctgggtcg aacggttggc
     4561 cggcatgttg gccggcatgg taggcaatcc ggcaagcctc gcagcaatac ttttgccagg
     4621 tggtacgctg ctcaaatgtc tggccacatt gcggacaatc ggcaacgcgg tagcccgatg
     4681 cgttataacg caatgcgtta ctgccgttgg gggcattggt tggcggtggc ggctgttggt
     4741 tggcgcggtt ggctgtaaaa ccgatttccc ggcgttctgc tttctgttgg tattggtata
     4801 agatgggcgg ttttcccggc tccggcggct ccggtgtgcc gtcctcaatg cgttttatca
     4861 atgcaaagat tttggcgtgg aaacgttcgg agagcgcggc cataaatgcg gcaaccggtg
     4921 actgccgaaa aaaatactcg gtgggtaggg cttcctcttg tatctcgctc ccgtgtgcgt
     4981 gtacctcttg caaggcaatg gccagcagca aaacggcaag gcacaacacg gtaaaccagg
     5041 cgacacttcc cccccaagcc gatacttgtt tttgccgttt ggcttcggcc tgggcgttgc
     5101 gctccttcag ggcggccagc cattcggagc gctcccggtt caggtcggca tttagggcgc
     5161 tgtgttcgtc ctggagcgcg gccatttcct ttgccccggc ctctgtgagc gcggccagct
     5221 tgagggcgtg ggcggcggtt gcgtcggcaa tggtttgttt ggtagtggct ttctgtgtgg
     5281 cgaaactttg gccggtattg cgctcccggc tttcaatgcc tttcagcttg gtattattgg
     5341 cgtcggtggc ggctttgtgg gccatttcaa gagctgcgat ctgtgaggca taccggccag
     5401 ctatggcggc gctatcgcgt cggtagcttg tcaggttgct ttcccggtgg ctgttgacgg
     5461 tcgtatctat tgccccggtt ccctctgttt tggggggcgg cacaacggcg gctactgttt
     5521 ctttactccc tgcaaatgac aggtagcccg atgcggtaag cagcaagagc gaagcggcaa
     5581 aaatggcaat gctcattacc aaatcaaggc cggcgaagcg cttgtttaaa atctgccttg
     5641 cggcgaaggg taaaaacttt ctcaaaccga tctcgataaa ggcggtggcc agttgggcgc
     5701 ccgcaatacc gacaacgccg gccaacatcg ggggggcaat gtcggcaacg gcgtttctaa
     5761 tgagggcata caaaatgcct gcttcggtaa gggcgcttac cacttgcgcc acataggaaa
     5821 gtttggagag cgtcggcaca agtccggcgt atcgttggaa aaaagcctca ttttgaggca
     5881 aggttaagcg aaaacgcata gttttgtaaa aattttctgt ggaaaattcc cggtcaattg
     5941 cttccaggcg tggccgggtt tttttatttc agtcagtgcg gtacgttggg gggggcggta
     6001 agtgcgggcg gctcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtggccttag cgtatgcggc ggcaatggcg
     6181 gcggcaaagt cgttttctct atacttgtcg aaaatcgtgt attgctcagg gtcatcgaac
     6241 ggcggttcga tctcgtaaaa ctcagcctct ttttccggct caggttgtag aatgatgtgc
     6301 agcgtcataa ctgattattt gatattgatt ttgtcgaaag tatcggcagc ggcgcggcgc
     6361 cgttcgtcaa cgatgcgggc gtaaacctga gtcgttttta cgtcgctatg gcccaacatc
     6421 ttagaaacca cctcaagcga aacgccttga gtaagtagta aagtggcgta tgtgtggcgg
     6481 gcggaatnnn nnnnnnnnnn nnnnnnnnnn nnnnaatgga aggtgatttt tttggagatg
     6541 ccggcggcta aaaaccaacg ttgaagcaaa atcatattgt tggcaaatat cttttccggc
     6601 agcgtgggaa atactttatc attgggcaag ccggccggcc ccaacaatgc gcgggcgcct
     6661 ttggatatgg gtaacgtttc gtttttttgg gttttttgtt gccgatagcg aagtgcaaag
     6721 ccggtttcct catctccctg gacttcgccc cacattagtt tgaaaatgtc gctgtatcgc
     6781 aggccggaca ttgccgaaaa taaaaacgcg ttcctcaagt ccaggcgctc gcaaggggta
     6841 acggccaggt gctccagttc ttccagtgtt aaatattctt tttgtgccgg gttcgattta
     6901 atcggctcta tgtaggccgt aaaatcttta ctcaaatagt ccaatttgta agcgtctttc
     6961 aaagtggttt tcaaagcggt aaggtaaagc gatttagagc tatcggcaag ccctttgagc
     7021 gctgccaaat ggtcacgaaa gcctataaca tctcgtttct caatggcggc caatgccaca
     7081 tcattgggcc aatattggcg taatattttg agcgtactcc accatgtgcc ccgcgttttt
     7141 tggttctttt tattggctct ttgctccata aactcaatga ggccggtctt aacccttgag
     7201 gcttttgaga agtcaaaagc ggcgttttgt acgtccaatt gacgttgggc gcggatgttg
     7261 tcggccaaag cgcgggtttc tttattgtgg cgggtttcgt ctggggtttt gggtttgtcg
     7321 taagtgaaaa gccccaagaa ctcacggcgg gtttcttttc ccgtcgaggt gtcaagtatg
     7381 ggcggccaaa aatccagaaa aagactttgc cggccttttg tcagcggctt gctccgaaga
     7441 tgaacgcggg gcgccttgcg attgctcata tttgtacgct gtttgatttc aggtacaaat
     7501 ataaagcgat tgtgtctata ataggtacaa aaaagccgaa aaaattaaac ggacatagct
     7561 caggtaacta acaatactaa tttcttaata tgttgatttt cagtattttt atatattttt
     7621 gtatgtttgt tgtttgtgcc ttgcttgtat cttattattt cggacgcaaa ggtagtgatt
     7681 gctgtttgtg aaaagaaagg aatatcaatg attccccaaa tgcccgccgc aaaatttgca
     7741 atacaccgca tctgcatcgt ggccttcgct caaacaatgc gggcacacct gcgtggtgat
     7801 tttttcgggt ggctttcgca tgatttccga agtaatgata ccggttggaa cggcaatgat
     7861 ggaataaccg atgatcatta acacactggc taaaaattgc cccagaggcg tgacggggga
     7921 aatgtcgccg tagcctaccg ttgtaatggt gacaatgcac caatacacac ttcgcggaat
     7981 actgtcgaag ctttcattga cgccgccctc cacgaaatac atcaccgagc cgaacacaaa
     8041 agtgaccaaa accaccacat acaaaaagat ggaaatcttg tagcggctgg ccttgagcgc
     8101 cccggctatg tgattgccgt gatccatcag accggtgagc ttgaaaatgc gaaacacgcg
     8161 caacagacgc agtatcctga tgaccatcag actctgtgcg ccccccaaaa tgaagctcaa
     8221 ataggtaggc aaaatagata ccagatcaat gatgccgaag aagctggcgg catatttcaa
     8281 acggttacca acggtatata atcgcagcaa atactcaatc gtgaagaaga tggtgaaaac
     8341 ccattctaat accacaaaaa gatggtgtac ctcatcggga aaagttttga cactctccag
     8401 catcaccacc aacacactca acagaatagc gactaacagg gaaacatcga atattttccc
     8461 tgccggcgta tccgcttcaa aaatgatggt aaaaagacgg tcccggagtg cttgccgatt
     8521 actgttttgg ttcataacaa aaggctatga cttttcgccc acgaagtagt cttctttgtc
     8581 cttgaacagc acattgaagg tggtgaggct gccgtatatt ttggtgatgt actgctggag
     8641 gttgactttg tcttcatccg tcagtttgct ggcattgatg cgttgctcca tcacgcggag
     8701 gcggtcgcgg gtcatcacaa ttttgtgaaa gaaactctca atgggtatct ctttttcttt
     8761 gagtgtattg tcggcgggct tcagaatcat catgccgcct tcccagcggt cgcccaattc
     8821 tacttcttcc tgcacggcat tgtagttgcg caggattttg agcagcgaac gctcggcctc
     8881 cgtaaaagtg acggcctcct cggcgggaag ggcttcaatg atttcccatt tgtcgtattc
     8941 cttgcccacg tgtttgatgc cgtagagcat aaaactcagt tcgtaagcgg ccttgtgcag
     9001 acgaatcact acgccgtcgc cataagccgg gtgccggacg cgggagccta ctcccaatag
     9061 tttttccaga ttttccattt tatctaaaca gttgaatttt aagacattca aggttcaagg
     9121 tttcgggttg ctcacgcctc acatactcac ctctcaccca ctcaccaact cacccctgct
     9181 cccgatactc caactccccc tccgaatgcg caataatcgt tgccaccgtt atgtcgctcg
     9241 ttacattggc cactgtgcga cacatgtcca gaatacggtc caccgggaag atgatggcaa
     9301 tccaagcggg gttgaggccc accgattgca gcacgatgat catcataata aggcccgcgc
     9361 tgggtaccgc cgccgagccg atactggcca gcgtagccgt cagcacgatg gtgagctgtt
     9421 gtgaaagcgt caggtctatg aggtgcaact gagccagaaa cacgaccgcc actgcctggt
     9481 acaaggctgt gccgtccata ttgacagtag cgccgatggg tagcacgaag ttggttacgt
     9541 ttttggaaac acccaaatta tcgcgcacac actccatgct caccggcagg gtggccgcgc
     9601 tggaactggt actgaatgcc agaaactgtg ccggactgat gcgtttgaaa aaggaagaat
     9661 aggagatttt tcttaccaac agcaaaatca ggccgggata aaacactgcc accaagagcg
     9721 ccaaaccggc cagcaaggtg agcgagtaag taagcaggcc tttgaatatt tccaccgcct
     9781 ctgccggtgt tttcgccatt ttggccaata cgcccgacat gagcgcgaac acaaaaaacg
     9841 gcgcagcttc catgatccat tccaccattt tgaggaatac ctcgttggcg ccgttgatga
     9901 catccagcac cggcttcatg gattcctttg gcaggaacat ggtgacgata ccgaaaaaca
     9961 acgcaaagaa aatgacctga agcatgcttt tggaatcgga cagggaggca aaaatattgt
    10021 cgggcaccat gtccacgaag aattggagcg ggccggcatt tttgaaagac tctgccgccg
    10081 ctttacggct ctccaccgtt tcgttgccct gttctttttc catttgaatg gcggcttctc
    10141 cggcaaaagc ggcatactcc ggttttgccc ggtaattctg gccatccaac acgccaacgc
    10201 cgggtgtttg ttcggcccag atttcgtatt tgatgcggtt tttgatgcgc tgttctttgg
    10261 caataaactc gccgggttgg aataaattgg ccaacaaaag ccccaaaccg accgctgtaa
    10321 tcgttgtaaa caggtacatc cccagggttt tggcgcccat gcggcccagc ttcgcaggat
    10381 cgggcagggt agaaacgccg ctgatgatgg aaaagagcac caaaggcacg gcgatgaact
    10441 tgagcaggcg gatgaaaatg acgccgaagg gatcaatcca atccaacgta aatttattcc
    10501 aaccgaaatg aatggaaacg aaagagtaga tgatccctgc tatgaggccc atgatgatgc
    10561 gccagtgcaa ggggatgttg cgttttttca tgtacctgtg ttttgatttg gagcgccaaa
    10621 atagttatca tctttcaaac ttctcaaatt ttctcacagc aggctttttt ccggcttttg
    10681 cttacctttg cgcttcctct tatgtgatct ttctcgcaaa gagaagaaaa tcaaaaaatt
    10741 atgggttggt tcaagcgact caaggaaggc atacagaccg caacgcgcaa caaaaaagag
    10801 gctcccgagg gcctttggta ccaatgcaaa gagtgcagag ctaccagcac gcgccgcgaa
    10861 ttgaaggaca atttttccaa atgtcccagt tgcaactatc acgttcgcat cagttctcag
    10921 gagtattttg aaatactctt cgacgggcag gcagatgagc ttttcggcga tctgcgctcc
    10981 aaagacatgc tcaatttcgt ggatttgaag ccttattcnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcaatt tcatcggtgg ttcgatgggt
    11161 tcggtgatgg gcgaaaaaat ttcccgcgcc gtagatcact gcctcgaaca caaaaccccg
    11221 ctgctcatca tctccaaatc gggcggcgcg cgcatgatgg aatcggcctt ttcgctgatg
    11281 caaatggcca aatcatctgc caaactctcc ctcttggtca aggccggcat tccttatttt
    11341 tcattgatga ccgaccctac cacgggcggc gtcacggctt cttttgccat gctgggcgac
    11401 attaattttg cagaacccaa agcgctcatc ggttttgccg ggccgcgcgt catcaaagaa
    11461 actatcaaac gcgagctgcc cgaagagttc caaacttcgg aatcgctgct cgaaaacggc
    11521 ttccttgatt tcatcgtaga tcgccaagac ctcaaagatc gcatggcaga tttgctggcg
    11581 ttttttgatg ccgctcctgt tgccaatggc agcgcagatg cagcgctgga ggcttctgcg
    11641 gagtgagggt gtgattcggt agccgcgccg tggcgcggta agcgtgaggg nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnga aaccttgagc ccgaaacctt gaacaaagcc cgttcctgcc ggaacgccaa
    11821 aattttgaac gcgaatctta gcgaagggcg cgaatttccg cgaatgcgag cgaaacgcgg
    11881 cttgcagctt gtagccagta ggcgttccaa cccggaaccc caaacccgct cctattcaaa
    11941 cacaaaatta ttgacgtaat ccggcgcttc ccagaagcgg cccggcccct gatgctgatg
    12001 tatggcagcg tcaaattcgg ccagcggcct caattttcct ttgtgaataa agcgccccgc
    12061 gtagcccaga tcgagcaaaa agcgaaacac atcctgtaca tcgccgccgc cgagatgccg
    12121 gttttcgcac tccatgagga tgcgcgggcg gcaggcttcc aagagggctt gcccaccttt
    12181 gagcagctcc cattcgtggc cttccacgtc tattttcagc agggccggcc ggatgccctg
    12241 agcgtggaaa tacgcatcca aagtactcac ttgcactgta tgatggtgaa aatcggtggg
    12301 gttgctctcg gtcacggcgt tcagggtagc gcccggcgag gtacccgacg aggaggccgg
    12361 aatgtgcagt gtcatgctgc cggtttgcga ggacaggccc atgttttcca attgtacatt
    12421 gttgaaattc aattgttttt gaataactgt gaggtattca aacaagcggg gttgcggctc
    12481 gaaagcatac acgcgcccat gcagccctac ccggcggcgc atccagtaga ggtagccgcc
    12541 tttgtggcag ccgatgtcca ccgccgtatc gccgggccgg agggtggaaa ggaggtaatg
    12601 gatttcggcg gggtccaccc ggaagcgata gcgccaggcc cggtacagat aagaaagtcg
    12661 tgacagcatg gtgatgaatt gagggggcaa gatacaggac gccggctgga aaatgcgtgc
    12721 aaccgggagg atttttgtga atttgtgggc ctgctgcggc agggacggca gcggatgcca
    12781 ggctgctccg tagcgtggtg gtttcgaacc ccgcgctatg gagcagccgg catggagcgt
    12841 acggcccggc gcccgccgca tagcaagagc tgttagcttt aaccggttta tgaagtgatg
    12901 aagctaacag ctctttgccg gtagcgggcg gcc
//
LOCUS       DAORLF010000069        12915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2585_length_12915_cov_2.032893, whole genome shotgun sequence.
ACCESSION   DAORLF010000069 DAORLF010000000
VERSION     DAORLF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_12915_cov_2.032893"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK198_07860"
     CDS             <1..1168
                     /locus_tag="PK198_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38688.1"
                     /translation="KKTKTTRYPHHRQKVMKGNYAAAEAAKLCRVDFVPAYPITPQTS
                     IVERIAEMCASGEMDATYINMESEHSVFAAAKGAATAGARVFTATAGQGLFYAHEVLH
                     AIAHLRLPIVACNVGRPSFPWNIWSDQSDSLSQRDTGWVQFYGESSQEVLDTVIQAYR
                     LAEQLNLPVLVVLDAFYQSHTSENVWVPDQDLTDAFLPAKQPGITAVDMEHPKAIGGL
                     VPPEHYGKFNHNFWQDACRVETAAEEIAAEFAQTFGREYHNVEAVNIGPQTKLVLVTV
                     SSITTTARGVIRSHSEVGLLKLRLFKPFPRQAVLEALRPLSDGARLAVIERNFMGDRE
                     GALYQEMQRALYGRPLSLLDFYAGVGGKDVPPTTVERIIQMALREDAEVVNWVE"
     gene            complement(1120..1518)
                     /locus_tag="PK198_07865"
     CDS             complement(1120..1518)
                     /locus_tag="PK198_07865"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF38689.1"
                     /translation="MRNFKKLLVWQKAMKIVELTYQMTDKFPDYEKFGLTSQCQRSAV
                     SIASNIAEGSSRRSEKDKYRFSEIALGSAFELETQVLVAQLRQYASPELIQALRLKAV
                     ATCQEPVACSRTNQPYSTQFTTSASSLNAI"
     assembly_gap    1626..1733
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1755..2651
                     /locus_tag="PK198_07870"
     CDS             1755..2651
                     /locus_tag="PK198_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF38690.1"
                     /translation="MEPSMKFLIQEEQVYSGALSCRGCGWSLLVRHLTDILGPETVYV
                     MPASCLSIISGPYPLNDLKANVVHTLFAASPATATGVRAALDRQGKQHVTVAVLAGDG
                     GTFDIGLQALSGAAARDENILYVVNNNGAYMNTGVQTSTATPYGAVTTTNPGAGYKRS
                     WPKDLMGIVAAHNLAYAATCSLAFLEDFRNKVQKAKSVHGFRLLMVDGPCPPGHKTEP
                     AQSIHVARMAVETRLFPLFEVERQHYAISYKPERFRPAVECLRLQGRFRPLFQPGNEQ
                     ALDDVQAHVEEYWARLEKLEES"
     gene            2715..>3211
                     /locus_tag="PK198_07875"
     CDS             2715..>3211
                     /locus_tag="PK198_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38691.1"
                     /translation="MRAFFLVVVALHALIHILGFVKAFGIREIEELTLPISKPLGAIW
                     LLAAALLLVYGVLYQLHSKPAWIVGLVGVEISQILVLYFWKDAQLGTLPNLLILVVLI
                     FSYANSMFDGLVREETLEVLVPAEAPAQTVLSLEETAYLPTPVQRWLVQCGAVGKPKA
                     TNGRV"
     assembly_gap    3212..3242
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3243..3811
                     /locus_tag="PK198_07880"
     CDS             <3243..3811
                     /locus_tag="PK198_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38692.1"
                     /translation="KDWYPATAIQHTNVSKPAFIWSVDLKMNPLMWFRGRDKLQNGEG
                     EMLIKMNALLTIVNECGEKLDEGSIQRLLGEMVWFPSLALSPYVKWEPIDDLSAKATI
                     AYQGTEGSGSFDFDEKGNFTRFVALRYQGNAPDSERKEWILTVDDYAVFEGIKVPSKM
                     KATWKLDSGDWTWLHLEILDIQYSILKI"
     gene            complement(3891..>5409)
                     /gene="katG"
                     /locus_tag="PK198_07885"
     CDS             complement(3891..>5409)
                     /gene="katG"
                     /locus_tag="PK198_07885"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRF38693.1"
                     /translation="NPDPIAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAADP
                     GKYVGREPAAGSIEEQSTGWKNTFGTGNAGDTITSGLEGAWTTTPTKWSNNFFWNLFG
                     YEWELTKSPAGAHQWIPKHGMGAGTVPDAHDPNKRHTPVMLTTDLALRFDPAYEKISR
                     RFHENPDEFADAFARAWFKLTHRDMGPRSRYLGPEVPAEELIWQDPVPAVNHPLIDDS
                     DIADLKSKILASGLTVGQLAATAWASASTFRGSDKRGGANGARIRLAPQKYWEANNPA
                     QLSKVLDTLEGIQAAFNTANAGGKQVSVADLIVLGGCAAVEQAAKNAGHDVKVPFAPG
                     RTDATQEQTDVESFAVLEPLADGFRNYAKASVAGVAENWLIDKAQLLTLTAPEMTALV
                     GGLRAMNANYDGSQHGVFTHRPEALSNDFFVNLLDFSTTWNATSDAQTAFEGRDRATG
                     KVKWTGTRADLIFGSNTELRAVAEVYACEDAQEKFVHDFVAAWTKVMNLDRFDLA"
     assembly_gap    5410..5425
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5426..6079)
                     /locus_tag="PK198_07890"
     CDS             complement(<5426..6079)
                     /locus_tag="PK198_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767456.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRF38694.1"
                     /translation="MSQQPTGKCPFHSAGGGTTNRDWWPNQLKLNILRQNSSLVNPME
                     ETFNYAEEFKTLDLAAVKQDIFNLMTTSQDWWPADYGHYGPFFIRMAWHSAGTYRIHD
                     GRGGAGSGTQRFAPLNSWPDNANLDKARLLLWPVKKKYGRKISWADLMILAGNCALES
                     MGFPTFGFAGGREDVWQPEEDIYWGSEAEWLGDKRYTGDRDLENPLAAVQMGLIYVNP
                     "
     gene            6493..7080
                     /locus_tag="PK198_07895"
     CDS             6493..7080
                     /locus_tag="PK198_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF38695.1"
                     /translation="MSNACNKTMQDMEVVNTYLETQASVCFSVLYNRYSGKIFGKCLA
                     LLKDEALAEDATQEVFTKIFLNLSKFGEKAKFSTWVYSITYNFCIDYLRRKKKMQNLF
                     ADDKEVEKLGDQVADEVPDEELMEMEVAQLRSVLDYIPVDDKAVLLMKYQDDMSIKDI
                     SELLNKTESAVKMKLKRAKEKAFRIKHEMFTEATL"
     gene            7077..7343
                     /locus_tag="PK198_07900"
     CDS             7077..7343
                     /locus_tag="PK198_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38696.1"
                     /translation="MKINNFKKILEEDEQKFLGARQDSCRKSIWGALGVYKFVGQLMD
                     VYVPKAVDVLISATNANSSKAAPSDGPEQNAGPGGPSSPGNGRI"
     gene            7388..8275
                     /locus_tag="PK198_07905"
     CDS             7388..8275
                     /locus_tag="PK198_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRF38697.1"
                     /translation="MEQQPDNIGGGGKAPDTPEAASSLLTKLQSEAEGLVSILPNIVG
                     AILVFLIGWLVAKTIARVIKKVLSKIGIDKLAERLNEIEIVYKSNIKIVPSILLSKIV
                     YYMLMLIVIMAATDVLKMKAVSDLVNNIINYIPSLLTAMMVLAIGILVADTVKNAVQT
                     ACTSLAIPAGKVISSVVFYFLFINVLMMALSQAQLETGFIESNISIILAGVVGAFAIG
                     YGFASRNIVASLLTSFYNKERVKIGDIIRIAGTEGRVMAIDSTSLTLQTHTGGEVVVP
                     LHKLSSEMFEIVGRRQDQG"
     gene            8294..9307
                     /locus_tag="PK198_07910"
     CDS             8294..9307
                     /locus_tag="PK198_07910"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRF38698.1"
                     /translation="MKYLLTATWLLLFSIGVFAQDAAPAPAEQEPAWKRTAGLGLDFA
                     QLFQLNPRQGAGQNRLGIGGAVNIAAAYKKNRISWDNKALWQFGVQRLGAGVIAQGNV
                     DKKIPFQKAIDELRINSKVGYAVSKDSKLSYAADVSFLSQLTPTYAGNAAFPGNFLTD
                     IVGNGANSKLFSPATLTLSAGFDYKPSPKLSIYYSPIGGKFIFVGDDAIAQLGVHGNP
                     VTRDAAGNVIDFENTFAGLGSLVRVNYANKFLKDRLAYGSGLVLFSNYLDGPQNIDVD
                     WTNQLDFSIIKGLQATMLLNVFYDHDVLVQITDNTAPNGVNGLGRRVSLTQQFLLKYS
                     LVF"
     gene            complement(9502..>11230)
                     /gene="yidC"
                     /locus_tag="PK198_07915"
     CDS             complement(9502..>11230)
                     /gene="yidC"
                     /locus_tag="PK198_07915"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRF38699.1"
                     /translation="AQAQPPVAVPDSVKAAQQASQLGVFGPAASGKAKEYTLENDVIK
                     IVFSSKGGRIQYAEMKQHFKVDEDDKKKEVKTPLRLLEDTKNKFEYLLPAAGSAKGVV
                     SSNDLFFTVEEAPNRLVFRADAGEGRFFEQSYTLSPDNYTLDYDIRFEGFSAIAPGGT
                     SEIKLNWVNYLDKLEKNHRFERNYSTVYFRPADDSPEYCSCTGDDSKDKNSVPLKWVS
                     HANQFFNTALVADNSFKGAVMETKMLDETDENLKKVRSEIRIPIKGDGAEAFGMTMYL
                     GPNEFNRLRAIGYNLEDVIPFGRSILGSINRWVVRPVFNFLLGLIGTKGIVILILTLI
                     VKMAVYPLTYRMLYSQSKMAALKPQLAHLKERYKDDAQQQQVETMKIYREYGVNPLGG
                     CLPSLLQMPIWIALYRFFPAAIEFRQAGFLWATDLSSYDVALRLPMELPMGFGSHLSA
                     FTFLWAISTLVYTYYSTRDMDFTANPAMKYMQYVMPLFFLGFFHSYASGLTLYLLFSN
                     LITIAQTIITRNYIIDKDKILAMMEEHRKKPKKKSGWQERLDQAMKEQQRIQAEREKG
                     KQAPKGGKK"
     assembly_gap    11231..11352
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11353..>11587)
                     /locus_tag="PK198_07920"
     CDS             complement(<11353..>11587)
                     /locus_tag="PK198_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38700.1"
                     /translation="QLVAKGFDLSACGGFCREKLNYRRWVLGKPDKNVPLRRFFQPKQ
                     SENSLLMDRNTIIGLLLIFALLMAWQQYMKPKA"
     assembly_gap    11588..11656
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11768..>11959
                     /locus_tag="PK198_07925"
     CDS             11768..>11959
                     /locus_tag="PK198_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38701.1"
                     /translation="MLSIFRTNQLLYSILLLMYAALLHLSVFVLREAWTPVAPGVFSA
                     WVYQLLGGASGMSAEITAIL"
     assembly_gap    11960..12031
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12032..12756
                     /locus_tag="PK198_07930"
     CDS             <12032..12756
                     /locus_tag="PK198_07930"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HRF38702.1"
                     /translation="FPGLFYILISSMLPGMMHLSPALMGNTFLIIAMSELFRTYKNPQ
                     ASGGIFNVGFWTAVASLFVPAYLVFLILGFVGLNSLRGLDLKERLMLLSGAVVPYGLA
                     GVVMFWNGQLLSGIHTQFQEAFAVASFVTITGFSFWAAASGFSLLVLLALTQFGGLVY
                     RKTIQVQKKVSLLYWMLLTGGLSTLVIKGIQIENWLSVAIPLGIVLGFVFSNLPRRWS
                     ESLHLMLLATVLILHYKQFLLP"
BASE COUNT         2932 a         3191 c         3308 g         3066 t
ORIGIN      
        1 aaagaaaacc aaaacgaccc gatacccgca ccatcggcag aaggtaatga aaggcaatta
       61 tgctgccgcc gaagctgcca aactgtgccg agtggacttt gtgccggcct atcccatcac
      121 gccacaaacc tccattgtgg agcgcattgc cgaaatgtgc gcatcgggcg agatggatgc
      181 cacctatatt aatatggaga gcgagcactc ggtatttgcg gcagccaaag gcgcagcgac
      241 ggccggggcg cgggtgttca cggctacggc cgggcagggc ttgttttatg cccacgaggt
      301 gctgcacgcc attgcccacc tgcggctgcc gatagtggcc tgcaacgtgg ggcggccttc
      361 atttccgtgg aatatctggt cggatcagtc cgattcgctt tcgcagcgcg ataccggctg
      421 ggtacagttt tacggcgaga gttcgcagga ggttttggat acggtgattc aggcatacag
      481 gttggccgag cagctcaatt tgccggtgct ggtggtactc gatgccttct atcaaagcca
      541 taccagtgaa aacgtgtggg tacccgatca ggatttgacc gacgctttcc tgcctgccaa
      601 acagcccggc atcaccgccg ttgacatgga acacccgaag gccatcggag gactggtgcc
      661 gccggaacac tacggtaagt tcaatcacaa tttctggcag gacgcctgcc gggtagaaac
      721 tgcagccgag gaaatcgccg ctgaatttgc ccaaacattc ggccgggagt accacaatgt
      781 ggaggccgtc aacatcgggc cgcaaacgaa actggtcttg gtcacggtgt ccagcatcac
      841 cactacggcg cggggcgtca tccggagtca ttctgaagta ggtctgctga agctgcggct
      901 gttcaaacct ttcccacggc aggcagtact ggaagccttg cgaccactgt cggacggtgc
      961 gcgccttgcg gtgatagagc gcaattttat gggagaccgg gagggcgctt tgtatcagga
     1021 aatgcagcgg gcactgtacg gccggcccct gagtttgctg gatttttacg ccggtgtggg
     1081 cggcaaggac gtgcctccga ctacggtgga acgcatcatt caaatggcgt tgagggaaga
     1141 tgcagaggtg gtgaattggg tagagtaggg ctggttggtg cggctacaag ctacaggctc
     1201 ttggcaggtt gcaacagctt tgagccgcaa ggcttgaatg agttcaggag aagcatattg
     1261 gcgaagttgg gctaccaaga cttgtgtttc taattcaaat gctgatccta atgcaatttc
     1321 agaaaatcgg tatttgtctt tttcactgcg tctgctactg ccttccgcga tattggaggc
     1381 tatacttact gctgatcttt gacactgaga agtcaagccg aatttttcat aatcaggaaa
     1441 tttatctgtc atctggtagg taagttctac gattttcatg gccttttgcc aaactaatag
     1501 ttttttgaaa ttcctcattg acttctattt tgaacaaaaa aaattcagga ccgatacgaa
     1561 tttgacaaaa gtacctgtaa atgccaaaac aacaacacac atatctcact tgcggcttac
     1621 ggcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaacaac
     1741 aaaaaacaac caccatggaa ccgagcatga aatttttgat tcaggaagaa caggtttact
     1801 ccggcgcact cagttgcaga ggctgcggct ggtcgctgtt ggtacgtcac ctcaccgaca
     1861 tactggggcc ggaaaccgtg tatgtcatgc ccgcttcctg cctgtccatc atatccgggc
     1921 catacccgct caatgatctg aaagccaacg tagtacatac cctttttgcc gcatctccgg
     1981 ccaccgctac cggtgtgcgc gccgcattag accggcaggg caaacaacac gtcacagtag
     2041 ctgtactcgc cggcgacggg ggcaccttcg acatcggctt gcaggcactg tcgggggccg
     2101 ccgcacgcga cgaaaacata ctttatgtgg tgaacaacaa cggcgcctac atgaacaccg
     2161 gcgtgcaaac cagcaccgcc acaccatacg gtgcagtcac taccacgaat cccggcgcag
     2221 ggtacaagcg ctcctggccc aaagacctca tgggcattgt ggcagcgcac aatctggcct
     2281 acgcagctac ctgctcgctc gcttttttgg aagacttcag gaataaagtg cagaaagcca
     2341 agtcggtaca cggcttccgg ctgctcatgg tggacggccc ctgcccgccg ggccacaaaa
     2401 ccgaacccgc ccagagcatc catgtagcgc gcatggcggt agaaacgcgg ctcttcccgc
     2461 tgttcgaggt ggaacggcag cactacgcca tcagctacaa acccgaacgc ttccggccgg
     2521 cggtggaatg tctgcgcttg caggggcgtt tcagaccgct gtttcagccc ggcaatgaac
     2581 aggcattgga cgacgtgcag gcgcatgtgg aggaatattg ggcaaggttg gaaaaattgg
     2641 aggaatcgta aaatcgggta cgtcgtcagc acgccgcggt ggataaagct tgtcttcatc
     2701 tcaaactacc cattatgaga gctttttttc tggtcgtcgt tgcgctacat gcgctgattc
     2761 atatactggg ctttgtcaaa gcattcggta tcagagaaat agaagaactg acgctaccta
     2821 tttccaagcc gctcggcgcc atctggctct tggctgcggc attgctgctc gtttacggcg
     2881 tgctttacca gttacattcc aaaccagcct ggatcgtagg gcttgttgga gttgaaatct
     2941 cacaaatact ggttctatac ttttggaaag acgcacaatt gggtacgctg cccaatttgc
     3001 tgattctggt agtgttgata ttttcttatg caaactcgat gtttgatgga ttggtacgtg
     3061 aggaaacatt agaggttctc gttcctgcgg aagccccggc tcagacggtt ttgtcgcttg
     3121 aagaaactgc ctatttgcct actccggttc aacgttggct ggttcaatgc ggcgctgtag
     3181 gcaagcccaa agcgacgaat ggaagggtga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnaaaaagac tggtatcccg ccacagccat acaacacacc aacgtcagca aaccggcatt
     3301 tatctggtcg gttgatctga aaatgaaccc tctgatgtgg ttcagaggcc gggataagtt
     3361 gcagaacggc gagggcgaaa tgttgatcaa aatgaacgct ctgctgacta ttgtcaacga
     3421 gtgcggcgaa aaattggacg aaggctccat acaaagactc ctcggcgaga tggtgtggtt
     3481 tccttcgctg gctctcagcc cgtatgtcaa atgggaaccg atagacgatc tcagcgccaa
     3541 ggcaaccatt gcataccaag gcacagaagg gagcggtagt tttgattttg atgaaaaagg
     3601 caatttcacc cgattcgtcg ctctgcgata tcagggcaat gcgcccgatt cagaaagaaa
     3661 agaatggatt ctgaccgtag atgattatgc cgtcttcgag ggtattaaag taccctccaa
     3721 aatgaaagcc acttggaaat tagacagcgg agactggact tggctgcatt tggaaatact
     3781 cgatatccag tatagcattc taaaaatata aataaaaagg cagcccgttg ttacaggctg
     3841 cctctggaaa tgaacaaatt gttatataag acaggataaa aaatctaaaa tcaagccaga
     3901 tcgaaacggt ccaagttcat cactttcgtc caggctgcta caaagtcgtg tacgaacttc
     3961 tcctgagcat cttcgcaggc atacacctct gccactgcgc gcagttcggt gttggagcca
     4021 aagatgagat cggcgcgggt acccgtccac tttaccttgc cggtggcgcg gtcgcggccc
     4081 tcaaatgccg tctgagcatc ggaagtagcg ttccaggttg tgctgaaatc caagaggttg
     4141 acaaagaaat cgttgctcag cgcttccggg cgatgggtaa aaacaccgtg ctgcgaaccg
     4201 tcgtaattgg cattcatggc gcgcaatccg cccactaatg ccgtcatttc aggagccgtc
     4261 agcgtgagga gttgggcctt gtcaatcagc cagttttccg ctacgccggc cacggatgct
     4321 ttggcgtaat tgcggaagcc gtcggccagc ggttcgagta ctgcgaagga ttccacgtcg
     4381 gtttgttcct gcgttgcatc ggtacgaccc ggcgcaaagg gtactttcac atcgtggcct
     4441 gcatttttag ctgcctgctc tacggctgcg caaccgccaa gtacgatcaa atcggccaca
     4501 gatacctgct tgccgccggc gtttgccgta ttgaaagcag cttgtatgcc ctccagcgta
     4561 tctaatactt tggacaattg cgccgggttg ttggcctccc agtacttttg cggagccagc
     4621 cggatgcgtg cgccgttggc gcctccgcgc ttgtcggagc cgcggaaagt agaagccgaa
     4681 gcccatgcgg tagcagccaa ttggcctacc gtcagccccg aagcaagaat tttggacttg
     4741 agatcggcga tgtcgctatc gtcaatgagc gggtggttga ccgccggaac cgggtcttgc
     4801 cagatcagct cttcggcggg tacttccggg ccgaggtagc gcgagcgcgg ccccatgtcg
     4861 cgatgcgtca gtttgaacca ggcgcgcgcg aaggcgtcgg caaactcatc agggttttca
     4921 tggaagcggc gcgaaatctt ctcataagca ggatcgaaac gcagcgccag gtcggtggtc
     4981 agcatcaccg gcgtatggcg cttgttggga tcatgggcat cgggtacggt gcctgcgccc
     5041 atgccgtgct tcggaatcca ttggtgcgct ccggccgggc ttttggtcag ttcccattca
     5101 tagccaaaga gattccagaa aaagttgttg ctccatttgg tgggcgtagt cgtccaggcg
     5161 ccttcgaggc cgctggtgat ggtgtcgccg gcgttgcctg tgccgaatgt atttttccaa
     5221 ccggtactct gctcctcgat agagcctgct gcgggttcgc ggcccacata cttgccggga
     5281 tcggcggcgc cgtgggtttt gccgaaggtg tgaccgcctg caatgagcgc tacggtttcc
     5341 tcgtcgttca tggccatgcg ggcgaaggtc tcgcggatgt cgcgggcggc cgctatggga
     5401 tcgggattgn nnnnnnnnnn nnnnncgggt tgacgtagat aagccccatt tgtacggcgg
     5461 ccagcgggtt ttccaggtcg cggtcgccgg tgtaacgctt gtcgcccagc cattcggcct
     5521 ccgaacccca gtagatgtct tcttcgggct gccatacgtc ttcacgaccg cctgcgaaac
     5581 cgaatgtcgg gaaacccata gattccaggg cgcagttgcc ggcaaggatc atgaggtcgg
     5641 cccaggagat tttgcggcca tattttttct tcaccggcca gagcaggagg cgggctttgt
     5701 ccaagttggc attgtcgggc cagctattga gcggagcaaa gcgctgagta ccggagcctg
     5761 ccccgccgcg tccgtcgtga atgcggtagg tgccggcgct gtgccaggcc atgcgaatga
     5821 aaaaggggcc gtaatggccg tagtctgcgg gccaccagtc ttgggaggtg gtcatcaaat
     5881 tgaagatgtc ttgtttgacg gctgcgaggt ccaaagtttt gaactcctct gcatagttga
     5941 aagtctcctc cattgggttg acgagagagg agttttggcg tagaatgttc aatttcaatt
     6001 gattgggcca ccagtctctg ttggtggtgc cgccgcctgc actgtggaag gggcatttac
     6061 ccgtgggttg ctgactcatg attttgattt tattttgatt atagatatgg ttcgagcgcc
     6121 ggatgcagca tggctgtttc tgtgaaagcg catgcaaatt taccacgccg gaaagtatcg
     6181 tcaaaatgaa tagtttcaat aaactgattg aggttgtcaa tatattacga cgcaagaccc
     6241 ggtttgcccg aaaggcaagt cggtagtttt aaaaaaatgt acctttgtgg ggcgattaaa
     6301 gtgtctttct tcgggcacga acgtctcata ggggcgataa aaaataaagt ggtcattttc
     6361 gccaaaggat tagataagta aacgtttcat tttgagtatc ttatctaatc ctttggccta
     6421 aagaaaatga acatttattt ttgcctccct acttaggtgc ttttttagat aaacagacaa
     6481 attgggttcc ccttgagcaa cgcatgtaac aaaaccatgc aagacatgga ggtggtgaac
     6541 acctacttag aaacacaggc ttccgtgtgc ttcagtgtat tgtacaaccg gtattcgggc
     6601 aagattttcg gcaaatgtct ggctttgctc aaagatgagg cattggctga agatgctacg
     6661 caggaggtgt ttacgaaaat tttcctcaac ctctccaagt ttggggaaaa ggccaaattt
     6721 tctacctggg tgtactctat tacttacaac ttctgtattg actacctgcg tcgtaaaaag
     6781 aaaatgcaaa atctttttgc cgacgacaaa gaggtggaaa aactgggcga tcaggtagcc
     6841 gacgaggtac ccgatgagga attgatggaa atggaagtgg cgcaactgcg ctctgtgctg
     6901 gattacattc cggtggacga caaagcggtg ctgctcatga agtatcagga cgacatgtct
     6961 atcaaagaca tttcggaact gctcaacaaa accgaaagcg ctgtgaagat gaagctcaaa
     7021 cgcgcgaagg aaaaagcctt ccgcatcaaa catgaaatgt tcaccgaagc gaccttatga
     7081 aaatcaacaa tttcaaaaaa atcctggagg aagacgaaca gaaattcctc ggcgccagac
     7141 aggacagttg ccgcaaaagc atctggggcg cgctgggcgt gtataaattt gtgggccaat
     7201 tgatggatgt gtatgtacct aaagccgtgg atgtactcat ctccgctacc aatgcaaact
     7261 catctaaggc agcgcccagc gacggccccg aacaaaacgc cggaccgggc gggccttctt
     7321 ctcccggcaa tggccgcatc tgacaataac gaaaacaaat atttatcaac tacaagcaag
     7381 caaactcatg gaacaacaac ccgataacat tggaggcgga ggcaaagcgc cggatactcc
     7441 cgaggcggct tcgtcattgt tgactaaatt acagtcggag gcggaaggtc tcgtttccat
     7501 ccttcccaat atcgtgggcg ccattttggt gtttctcatc ggctggttgg tagccaaaac
     7561 cattgcccgc gtcatcaaaa aggtgctgag taaaataggt attgacaaat tggcggaaag
     7621 gctcaacgag atcgagattg tttacaaatc caacatcaag attgtaccca gtatcctgct
     7681 atctaagatc gtttactaca tgttgatgct catcgtcatc atggcggcta ccgacgtgct
     7741 gaaaatgaag gcggtttccg atttggtcaa caacatcatc aactatattc ccagtctgct
     7801 cacggccatg atggtgctcg ccatcggtat tttggtggcc gacacggtga aaaatgccgt
     7861 tcagaccgca tgcacttctc tggccattcc tgcgggcaaa gtcatctcca gcgtggtatt
     7921 ttacttcctg tttatcaatg tgttgatgat ggctttgtcg caggcgcagt tggaaacggg
     7981 cttcatcgaa agcaatattt ccatcattct ggccggcgtt gtgggagcat tcgccattgg
     8041 ctacggcttt gcttcgcgca acatcgtggc cagcctgctc acttcttttt acaataaaga
     8101 acgcgtcaaa atcggcgaca tcatccgcat tgccggtacg gaaggccgcg tcatggccat
     8161 agacagcaca tcgctgaccc tgcaaactca taccggcgga gaggtggtag tgcccctgca
     8221 caagctcagt tcggagatgt ttgaaattgt gggccgccga caggatcaag gttaaccaaa
     8281 acatcacaat aacatgaagt atttactcac tgcaacctgg ttgctcctct tctccattgg
     8341 cgtctttgcg caagatgccg ccccggctcc tgccgaacag gaacccgcct ggaaacgcac
     8401 cgcaggcctc ggtctcgact tcgctcagtt gttccaactc aacccgcgtc agggcgccgg
     8461 tcaaaatcgc ctcggtatcg gcggcgctgt caatatagcc gcagcttata aaaagaaccg
     8521 catatcctgg gacaacaaag cgctctggca attcggcgta cagcgtctcg gcgccggcgt
     8581 cattgcgcaa ggcaacgtgg acaaaaaaat tccgttccaa aaagccatag acgaattgcg
     8641 catcaactct aaagtcggtt atgccgtcag caaagactcc aaactgtctt atgccgccga
     8701 cgtctccttc ctgagccaac tgacgcctac ctacgcaggc aacgctgcct ttcccggcaa
     8761 cttcctcacc gacatcgtgg gcaacggcgc caactccaaa ctgttctcac cggctacact
     8821 caccctttcg gcgggtttcg actacaaacc atcgccaaag ctttcgattt actactctcc
     8881 gataggcggc aagttcattt ttgttggaga cgatgccatc gcccaacttg gcgtacacgg
     8941 caaccccgtt acccgcgatg cagccggcaa cgtcattgat tttgagaata cttttgctgg
     9001 tttgggttct ttggtgcgtg tcaattacgc caacaaattc ctcaaagacc gcctcgctta
     9061 tggttccggc ctcgttctgt tttccaatta tttagacggc ccgcaaaaca tagacgtgga
     9121 ctggaccaac caattagact tctccatcat caagggcttg caggcgacta tgctgctcaa
     9181 cgtcttttac gatcacgacg tgctggtgca aatcaccgac aacaccgcac ccaacggcgt
     9241 caacggcttg ggccgccggg tgagcctcac acaacagttt ttgctgaagt attctttggt
     9301 gttttgataa aaacggggct attgaagggg tgactggcag tcacctcttc aatacaaaat
     9361 acactgaggt agccaacaaa aaagctcgaa cgggagagat tctcgttcaa gctttttttt
     9421 attgcaatat gctgaatgaa gggctgactc cggctgattc aactcagtag tcgccccttc
     9481 aatgacgatc tgttacaatc attacttctt ccctcccttg ggcgcttgct tgcccttttc
     9541 tctttctgcc tgaatgcgtt gctgctcctt catggcctgg tcgaggcgtt cctgccagcc
     9601 gctctttttc ttgggctttt tgcggtgctc ctccatcata gctaagattt tgtctttgtc
     9661 aatgatgtaa ttgcgggtaa tgatggtttg cgcaatggtg atgaggttgc tgaaaagcag
     9721 gtacagcgtc aggccggaag catagctgtg gaagaaaccg aggaagaaca agggcatcac
     9781 atactgcatg tatttcatgg ccggattggc ggtgaaatcc atgtcgcgcg tgctgtaata
     9841 agtataaact aatgtggaaa tggcccacag gaaggtgaat gcactgaggt gcgagccaaa
     9901 tcccatcggc aattccatcg gcaacctgag cgccacgtca taacttgaca aatcggtggc
     9961 ccacaagaag ccggcctgcc tgaactcaat ggcggcgggg aagaagcgat acaaggcaat
    10021 ccagatgggc atttgcaaca agctcggcag gcagcccccc aatggattga cgccgtactc
    10081 gcggtagatc ttcatggttt ctacctgctg ctgctgtgcg tcgtctttgt agcgctcttt
    10141 gaggtgcgcc aactgaggct tcagggcggc cattttcgac tgagagtaca acatgcggta
    10201 ggtgagcgga tacacggcca tcttgacgat gagcgtaagt atcagaatca caatgccttt
    10261 ggtgccgatg aggccgagca aaaagttaaa taccggacgc accacccagc ggttgatgct
    10321 gcccaaaatg ctccgtccga aaggaatcac atcttccagg ttgtacccaa tggcccgcag
    10381 gcgattgaac tcgttaggcc ccagatacat ggtcattccg aaagcctcag cgccgtcgcc
    10441 tttgatggga atgcggattt cggagcgtac ttttttcagg ttttcgtcgg tttcgtccaa
    10501 cattttggtt tccatcacgg cgcctttgaa actgttgtcg gccacgagcg cggtgttgaa
    10561 aaactggttg gcgtgcgata cccacttgag cggcacgctg tttttgtctt tggagtcgtc
    10621 gccggtgcaa gagcagtatt cgggggagtc atcggcaggc cggaaataga cggtagagta
    10681 attgcgctcg aagcggtggt tcttctccaa cttgtctaaa taattgaccc agttgagttt
    10741 aatttccgat gtgccgcccg gcgcgatggc cgaaaagccc tcgaaacgaa tgtcataatc
    10801 gagcgtgtag ttgtcgggac tgagggtgta gctttgttca aagaagcggc cttcgcctgc
    10861 atctgctctg aaaaccaagc gattgggagc ttcctcaacg gtaaaaaata aatcattgga
    10921 actgaccacg cctttggccg aaccggctgc cggcagcagg tattcaaatt tatttttcgt
    10981 gtcttccaac aagcgcaagg gagtttttac ctcctttttt ttgtcgtctt cgtccacttt
    11041 gaaatgctgc ttcatctcgg cgtactgaat gcgaccgcct ttgctggaaa aaacaatttt
    11101 gatcacatcg ttttccaatg tatattcttt ggccttgccc gatgccgcag gtccgaacac
    11161 gcccaactga gaagcctgtt gcgctgcttt tacagaatcg ggaaccgcta caggcggctg
    11221 agcctgagct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nntttgcttt gggcttcatg tattgctgcc aggccatcag cagggcaaaa
    11401 atcaggagca aacctataat tgtattccta tccattaata atgagttttc agattgtttt
    11461 ggctgaaaaa agcggcgcaa aggtacgttt ttatcaggct tgcccaatac ccatctacga
    11521 taattcagct tttctcgaca aaagcccccg caggcactta aatcaaagcc tttagccaca
    11581 agctgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnggat tgcaactgtt ggccttcagc cgaaggcata ccaaccccaa
    11701 gctcgcggct cgcagttcgc agcctacagc ttttttccta tcttcgcccc atcaaatcga
    11761 acaacaagtg ctatccattt tcagaaccaa ccaactcctg tacagcatct tattgctgat
    11821 gtatgccgcc ctgctgcacc tgtcggtgtt tgtgctccgg gaagcctgga cgccggtagc
    11881 gccgggtgtt ttcagtgcct gggtttatca actgctcggt ggcgcttcgg gcatgagcgc
    11941 cgaaataact gctattttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctttccag ggctttttta catcctcatc
    12061 agcagcatgt tgccgggtat gatgcacctc tcgccggcgc tgatgggcaa taccttcctc
    12121 attatcgcca tgagcgaatt gtttcggaca tataaaaatc cgcaggcatc gggaggcatt
    12181 ttcaatgtgg gattctggac ggcggtggca tcgttgtttg ttccggccta tttggttttc
    12241 ctgatattgg gcttcgtagg actgaacagt ctgcgcgggt tggacctcaa agaacgcctc
    12301 atgctgctgt ctggagcagt agttccttac gggctggccg gtgttgttat gttttggaac
    12361 gggcagttgc tcagcggcat acacacccag tttcaggagg cgtttgcagt ggcatctttc
    12421 gtcacgatta caggattttc cttctgggct gctgcgagcg gcttttcact gttggtgttg
    12481 ttggccttga cgcagtttgg cggcttggtg tatcgcaaaa cgattcaggt acaaaaaaag
    12541 gtcagcctgt tgtactggat gttgctcacc ggcgggcttt ccaccttggt catcaaggga
    12601 atacagatag aaaactggct ttctgttgcc attccattag gcatcgtttt gggatttgta
    12661 ttttccaatc tgccccgtcg ttggtcggaa tcccttcacc tcatgctgtt ggcaacagtg
    12721 ctcattttgc attacaaaca gtttttactg ccctgatttc agtagtccaa tcctgaaaaa
    12781 catgacgcaa ccaaccatag ataccttccc tttaagggct ttcgataagg tgagatatgc
    12841 cgatacagat cggcaggggc atgttaacaa cgccgttttt cctacttttt tggaaacggg
    12901 gcgggtagaa tttct
//
LOCUS       DAORLF010000070        12880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2595_length_12880_cov_2.198285, whole genome shotgun sequence.
ACCESSION   DAORLF010000070 DAORLF010000000
VERSION     DAORLF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2595_length_12880_cov_2.198285"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="PK198_07935"
     CDS             complement(<1..1590)
                     /locus_tag="PK198_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38703.1"
                     /translation="MSTVLVTAFYLRPGHGIGDGPIISVDDSRRFPAVFYDPARVMAS
                     MPGVAVANDQANGISLRGHSPNAVSWRLEGAEIVNPNHTPNAGTFSDRVSAYGGGVNI
                     LSSQLLSNSQLYNGPFPPAFGNALSGILDMQLRPGNNERREYTVQAGLIGLDVAAEGP
                     FSQKSKASWLINYRYSTIGLLAALGVPLGDEKINFQDLSFHLVFPGKNGARWTVFGLG
                     GNSVNIFEASRDSTTWEFQKDRYDIRFRSRMGAAGVTHSMPVGKRGQWSTVLSLSALE
                     STRSGDRLDDELRLLRLENDQYDQSKHSLNTVFRYRIGKMHRLQTGLLLTRQFFNIAS
                     VASDTGAYGQGGGWLCQPYVNWSSELSEKWRLDAGLHSAFFGFNGSRSLEPRLSLEWR
                     PSYQHRVTIAYGLHSQLQQPQLYFAGNGAHLNSQLGFTRSHHYRLGWSYSVARSLAIQ
                     VETYYQRIFDVPVETARPSSFSALNMEEGFVQTALANSGTGRNYGVELGLRKYLDRNY
                     WWLANATWYHARYTGSDGIERS"
     assembly_gap    1613..1777
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1925..2491)
                     /locus_tag="PK198_07940"
     CDS             complement(1925..2491)
                     /locus_tag="PK198_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38704.1"
                     /translation="MFGEIIGVDANSNADSPTAQMVMNDAGKMIEREWLNLKNRFQNI
                     ELHEYVVMPNHFHGILQIVEATLVVAQNEMTAAGAAGDTGDTGAGQPQGIAPTTAPTT
                     APTTAPTTENTPKNKTVGDMMDAFKSITTVEYIRGVTNLGWPRFDGKLWQRNYYEHII
                     RDERSYLNISHYIIKNPSKWWSDKFNSK"
     gene            2666..>3002
                     /locus_tag="PK198_07945"
     CDS             2666..>3002
                     /locus_tag="PK198_07945"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF38705.1"
                     /translation="MSQSTATYATVILPLALPKPFTYEVPAELRERVQFGVRVEVQFG
                     RTTAAGQGGKLYSALVIEVHQRAPEAYQPKPVLDVLDDEPLIHSAQVQLWQWMARYYC
                     CTLGEVMHAA"
     assembly_gap    3003..3016
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3017..>4266
                     /gene="priA"
                     /locus_tag="PK198_07950"
     CDS             <3017..>4266
                     /gene="priA"
                     /locus_tag="PK198_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF38706.1"
                     /translation="LSGETRLSLHPLHSGDLTGLSDREYLVAEALTIQESITIEDVRD
                     ILERKTVYPVIKSLLEKKIVLLQEDLQEKFKPKTVICVELAEPYASDPTKLNQAFELT
                     SRSSKQTTALMAFIQLSKQQSFVLRQEVCELADVENPSVKAIADKGIFRLYEREVSRL
                     GSYEEDTIDADALSPQQEKALGEIRGHFEQKNTVLLHGVTGSGKTKIYMELMSEVIAR
                     GGQALYLLPEIALTTQLTARLQRYLGDQLAVYHSKLNNNERVELWNAVLAGKPVVMGA
                     RSALLLPFSRLELIIVDEEHDPSFKQNDPAPRYNGRDSAIYLAQLHGAKVLLGTATPS
                     VESYHNALSGKYGLVEMTERFGGVQMPEIVLADARQEMKQRQMQSHFTTQLLNELKAA
                     IERGEQAILFQNRRGYAPTYRCFS"
     assembly_gap    4267..4276
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4277..5152
                     /gene="priA"
                     /locus_tag="PK198_07955"
     CDS             <4277..5152
                     /gene="priA"
                     /locus_tag="PK198_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF38707.1"
                     /translation="GWHSECIHCDVSLTYHKFSHLLKCHYCGYQSKLPPECPACGNRQ
                     LTLYGFGTEKIEDELKIYLPDARIARLDLDTARTRNAHAAIINDFEERRFDILVGTQM
                     VTKGLDFENVGIVGVLSADQLLQFPDFRSAERGFQLMVQVAGRAGRKNKRGKVIIQAM
                     NTAHPVLQEVIANDYKGFFQREIAERRAFRYPPFTRLIRITLKHKAPEVLNAGAEIFT
                     KALHQSLGDWVTGPAAPYISRVRGLYLLDYLIKMERDPRKMEFAKSSIDTATHILQST
                     EGFSGVRVVVDVDPG"
     gene            5200..>6423
                     /locus_tag="PK198_07960"
     CDS             5200..>6423
                     /locus_tag="PK198_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF38708.1"
                     /translation="MTADTQRAIAYERGALSNDQLIQLYERLLFPRMVEEKMLILLRQ
                     GKISKWFSGIGQEAIAVGVTAALLPDEMLFTLHRNLGVFTVRDIPLSRLFCQWQGKDS
                     GFTKGRDRSFHFGALEYGIVGMISHLGPQLSLAGGVALAHKLSGEGRVSVAFSGEGGT
                     SEGEFHEALNTAAVWQLPVIFVVENNGYGLSTPTTEQYRCENIADRAIGYGMRSCIID
                     GNNILEVYNTVSQLAAELREHPEPVLLECRTFRMRGHEEASGTKYVPKELMEHWALKD
                     PLANYEQFLLAENVLSEADVKAVRKRFKDHIEAELNIAYAEGDVTSTEADELADVYAP
                     WEQELTQPVGTAEDKRLVDAISDGLREAMRRHDKLVLMGQDIADYGGVFKITDGFVKE
                     FGKDRVRNTPICESAI"
     assembly_gap    6424..6433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6434..7202
                     /locus_tag="PK198_07965"
     CDS             <6434..7202
                     /locus_tag="PK198_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF38709.1"
                     /translation="AAIGLSLKGYKAMVEMQFADFVSCAITQIMNNAAKLHYRWGQPA
                     DIVIRMPTGAGTGAGPFHSQSNEAWFAHTPGLKVLYPSNPYDAKGMLLAAFEDPNPVM
                     YFEHRALYRSISGPVPQGYYTVEIGKAATVREGRELSVITYGMGVHWALKAVEALGID
                     AEIIDLRTLLPLDFSAIDATVKKTNKVLVLHEDTMFGGLGGEISAYITEHLFEHLDAP
                     VLRVASLDTPIPFSPAIERIFLPEERLRKQLERLRGY"
     gene            7295..7615
                     /locus_tag="PK198_07970"
     CDS             7295..7615
                     /locus_tag="PK198_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF38710.1"
                     /translation="MELIHKYQKALYKACSAHNVEELYTFGSILTDKFNAESDIDFIV
                     LIRSEDPIEYAENYFELKFELENIFNRKIDLLEQKAIRNKRFEALINRQKVLVYARGN
                     QGLA"
     gene            7587..7925
                     /locus_tag="PK198_07975"
     CDS             7587..7925
                     /locus_tag="PK198_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRF38711.1"
                     /translation="MQEEIRVWLEDIEKAVGEINGFLPEPRNFIDFVKDLKTKRAVER
                     NIEIIGEAMSRILNMQPDIPISNSRKIVDTRNRIIHGYDSVSDDVIWLIVSRYLPILE
                     AEIKDIQRGN"
     gene            8010..8966
                     /gene="metF"
                     /locus_tag="PK198_07980"
     CDS             8010..8966
                     /gene="metF"
                     /locus_tag="PK198_07980"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRF38712.1"
                     /translation="MKVTDYFDKATGQTLISFEVLPPPKGGGMQAIFSVLDPLMEFKP
                     PFIDVTYHREDFVYVERPGGYFEKISIRKRPGTVGICAAIVHRYGVDAVPHLICGGFT
                     KEDTENALIDLNFLNINNVLALRGDARKFDGRFVPEEQGHAYALDLVKQIADMNAGRY
                     LDENIENGERTDFCIGIAGYPEKHFEAPNMELDLEHTKAKVDAGANYIVTQMFFDNQK
                     YFEYVQRCRDIGIQVPIVPGLKPITKEGQLNAIPRNFFIDLPHELVSSVQQAKNAEAK
                     RQAGIEWCIAQSKELKAAGVPCLHYYTMGDAKTTRQIAEAVF"
     gene            9018..>10217
                     /gene="der"
                     /locus_tag="PK198_07985"
     CDS             9018..>10217
                     /gene="der"
                     /locus_tag="PK198_07985"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRF38713.1"
                     /translation="MSNIVAIVGRPNVGKSTFFNRLIGERQAIIDDMPGVTRDRQYGA
                     SYWNGKNFTVIDTGGFVEHSDDVFEAAIRSQVRIAIEEASVIVFMVDVTTGITDLDED
                     VAGLLRRTDKPVYLAVNKVDNAERFMEANEFWSLGIENTFFLSSLTGSGSGELLDAVV
                     DHFEEEERTESALPRIAIMGQPNVGKSSLTNALLGEDRNIVTEVAGTTRDSIHTHYSK
                     FGKEFLLIDTAGIRKKAKVHENIEFYSVMRAIRAMEESDVCILMIDAQTGMEMQDISI
                     FRLAQRRNKGIVILVNKWDLVEDKETNTARDFERAIHQRIAPFTDVPIIFVSATEKVR
                     IFKAMEAALEVFENRSRKIKTSELNEVMLEAIQHYPPPAYRGRLIKIKYVTQLPPAYP
                     AFAFFCNH"
     assembly_gap    10218..10235
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10270..10329
                     /locus_tag="PK198_07990"
     CDS             10270..10329
                     /locus_tag="PK198_07990"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38714.1"
                     /translation="MRKHFKFTGSPISIFFREK"
     gene            10339..>10575
                     /locus_tag="PK198_07995"
     CDS             10339..>10575
                     /locus_tag="PK198_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRF38715.1"
                     /translation="MPFLSTPIKGLIIFEPQVWEDHRGYFFESYNRRTFEAAGISADF
                     VQDNQARSTYGVLRGLHFQKPPFEQAKLVRVTEGA"
     assembly_gap    10576..10639
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10640..10871
                     /gene="rfbC"
                     /locus_tag="PK198_08000"
     CDS             <10640..10871
                     /gene="rfbC"
                     /locus_tag="PK198_08000"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRF38716.1"
                     /translation="ELSAENKRQLFVPRGFAHGYVVLSPTAEFCYKCDNFYSKDHDAG
                     IRFDDPALQIEWPIKLQDAVLSEKDLALPFYL"
     gene            10971..11261
                     /locus_tag="PK198_08005"
     CDS             10971..11261
                     /locus_tag="PK198_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38717.1"
                     /translation="MDYLFLVLTIPAYILAVVLWKARIRLKRIGEYKSFLFVAINAFL
                     GILGAQIGSFQALFLEKKFQIENSYGLSLLIMTGVVALLYFSFVYSKREVEA"
     gene            complement(11432..>12880)
                     /locus_tag="PK198_08010"
     CDS             complement(11432..>12880)
                     /locus_tag="PK198_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38718.1"
                     /translation="EIINSTFYLSDTIIAPFINKRNSQVISINYPDTLLLRNSIFAYS
                     DTVDRPYIFGRNTWASIQNCLFWADSCTQIFETFPVEIIIDPNTPPIYQYLGGLECSD
                     NIFASGHPFQNVESGDFSLRPCSPPVNAGSNAWLGSSDTLDAAGGLRVINGIVDMGAL
                     ELPALPGISISEQHIIPVNCATGTLGSIAILPDTARGPLEFQWSNGAMEPILQQLPPG
                     MYDLTITDADGCDTTLVFDMPIAGSLPVAVTVSPIICRGDANGRVVASPVYTALPQSF
                     VWNTGAQTPDINQLGAGQYSVEITNGLGCTGQAAILMEEPDSLSTTAEITPAGNSSGG
                     SIVITSVQGGRAPYELRWSNGATNDRLENIPAGAYALSVTDAGGCTRVFNFVVGMVNA
                     ATAVQDISIKIKLFPNPASEWLHFELEHEWIGIFYLEIYNSQGQSLLRFPLEKNNQRI
                     SFQADIAALPSGVYTARLRGAAISAGLKFVKM"
BASE COUNT         3006 a         3451 c         3349 g         2793 t
ORIGIN      
        1 gctgcgctct atgccgtccg accctgtgta gcgggcatgg taccaggtgg cattggccaa
       61 ccaccagtaa ttccggtcca agtacttccg caggccgagt tctacgccgt aattgcggcc
      121 cgttccactg ttggccaaag ccgtttgtac aaaaccctcc tccatattga gcgcagagaa
      181 agaacttgga cgcgcagtct ctaccggcac gtcgaaaatg cgctggtagt acgtttcgac
      241 ttgtattgcc agcgagcggg caacggagta gctccaacca aggcggtagt gatgcgaacg
      301 ggtaaaaccg agttggctgt tgagatgggc gccgtttccg gcaaagtaca attgcggctg
      361 ctgcaactgg ctgtgcaaac cgtaggcaat ggtcacgcga tgttggtaag atgggcgcca
      421 ttccaatgaa agccggggtt cgaggctgcg gctgccgttg aaaccgaaaa aagcgctgtg
      481 cagtccggca tccaacctcc atttttccga caactccgac gaccagttga cataaggctg
      541 gcagagccaa ccgccgccct gcccgtaagc gcccgtatcg gaagccacag aggcgatgtt
      601 gaaaaattgg cgcgtgagca acaggccggt ttgcaggcgg tgcattttgc cgattctgta
      661 ccgaaagacg gtgttcagcg agtgtttgct ttgatcgtac tggtcgtttt cgaggcggag
      721 gagccggagt tcgtcgtcta agcgatcccc gctgcgcgta ctttcgaggg ccgacagcga
      781 cagcaccgta ctccattgcc cacgcttgcc cacgggcata gagtgggtga cgccggccgc
      841 gcccatgcgg gaacggaagc ggatgtcata gcggtctttt tggaactccc aggtcgtgga
      901 atcgcgggaa gcctcgaaaa tattgacgct gttgccaccg aggccgaaaa cggtccatct
      961 ggcgccgttt ttacccggaa acacgaggtg gaaagacagg tcctgaaaat tgattttttc
     1021 atcgcccagc ggcacgccca atgccgccag cagcccaatg gtggagtagc ggtaattgat
     1081 caaccaggat gcttttgatt tttgggaaaa cggcccttct gccgccacat ctaagccgat
     1141 gaggcccgcc tgcacggtgt attcccggcg ctcattgttg ccggggcgca gttgcatgtc
     1201 gagtatgccg gagagggcgt tgccgaaagc cggcgggaac ggcccgttgt agagttggga
     1261 gttggaaagc agttgcgaac tgaggatatt gaccccgccg ccgtaggcgc tcacgcggtc
     1321 gctgaaggta cctgcattgg gcgtgtggtt ggggtttaca atttctgcac cctccaatcg
     1381 ccaagagacg gcgttgggcg aatggccgcg cagggagatg ccgttggcct ggtcgttggc
     1441 cactgccacg ccgggcatgg aggccatcac gcgggcggga tcgtaaaaaa ctgcggggaa
     1501 ccgccgcgaa tcgtccacgc tgatgatggg gccgtctcca atgccgtgac caggtcggag
     1561 gtagaacgcc gtcaccagta cggtactcat agagtctgct ttcgggcgca gcnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa tggtggcgcc ggtgaggtgc
     1801 ctgcccgtat ctgcgtcggt gatgtggccg cgcagggttt gagtgggcgt ttgggcggct
     1861 gcgaggaagg gcagaaggga gaggaggagg aagatgtatt tgattgtatt gagcatgtgg
     1921 agattcattt gctattgaat ttgtcggacc accatttgga ggggtttttg atgatgtaat
     1981 gtgaaatgtt gaggtaagat cgttcgtctc gaatgatgtg ttcgtaataa ttgcgctgcc
     2041 acaatttgcc gtcgaatcgg ggccagccca agttggtgac tccgcgaata tattctactg
     2101 tcgtgatgga tttgaatgcg tccatcatat caccgacggt tttattcttt ggggtgtttt
     2161 cggtcgttgg ggcggtcgtt ggggcggtcg ttggggcggt cgttggggca atcccttgtg
     2221 gttgccccgc gcccgtatcg cccgtatcgc ctgcggcgcc tgcggcggtc atctcgtttt
     2281 gggcgaccac aagggtcgcc tctacgattt gtaaaatgcc gtgaaaatga ttgggcatga
     2341 cgacgtattc atgcaattcg atgttttgaa aacggttttt caaattcaac cattcccgtt
     2401 caatcatttt cccggcatca ttcataacca tttgtgctgt cggactatcg gcattgctgt
     2461 tggcatccac tccgataatt tctccgaaca aacattcccg attttgcacg catattgtaa
     2521 taaaatacaa acccgcccgc gaataatcat accccttgag gcgtatggat ttcctgtgga
     2581 atttcttgcg atcagacgcc ataattctaa tttcttgcgg ccaaagttag tatttttgca
     2641 cctactcttc actcgtcctt accgtatgtc ccaatccact gccacctacg ccaccgtcat
     2701 tctgcccctc gctttgccca agccttttac ctacgaggtg ccggctgaat tgcgcgaacg
     2761 cgtacagttc ggcgtgcggg tagaggtgca attcgggcgc actacggctg ccgggcaggg
     2821 cggaaaactc tactcggcat tggtcatcga ggtgcatcag cgcgcgccgg aggcctacca
     2881 gcccaagccc gttctggacg tattggacga cgagccgctc atccacagcg cacaggtgca
     2941 actctggcaa tggatggccc gctactattg ctgcaccctc ggcgaagtga tgcacgccgc
     3001 gcnnnnnnnn nnnnnnactc agcggcgaaa cccgcctcag cctgcacccc ctccacagcg
     3061 gcgacctgac cggcctctcc gaccgcgagt acctcgtagc cgaagccctc accattcagg
     3121 aaagcattac catcgaggac gtacgcgata ttctggagcg caaaaccgtc tatcctgtca
     3181 tcaaaagcct gttggaaaaa aagatcgtcc tcctgcagga agacttgcag gaaaaattca
     3241 aacccaaaac cgtcatatgc gtggaattgg ccgaacccta cgcctccgat cccacgaagt
     3301 tgaaccaggc ttttgaactg acgagccgct cctccaaaca gaccaccgcc ctcatggctt
     3361 ttattcaact gtctaaacaa cagtcttttg tgttgaggca ggaggtttgt gaattagccg
     3421 acgtggaaaa tcccagcgtc aaagccattg ccgacaaagg tattttccgg ctctacgagc
     3481 gcgaggtgag ccgcttgggc agctacgagg aagacaccat agacgccgat gccctctcgc
     3541 cgcagcagga aaaagcgctg ggtgaaattc gcggccattt tgaacaaaaa aacaccgttt
     3601 tgctgcacgg cgtcacaggt tcgggcaaga ccaaaattta tatggaactg atgagcgagg
     3661 tcatcgcgcg cggcggtcag gcactctacc tgctgcccga aattgccctc accacgcagc
     3721 tcaccgcccg ccttcagcgc tacctcggcg accaattggc cgtgtatcac tccaaactca
     3781 acaacaacga gcgcgtagaa ctctggaacg ccgtactcgc cggcaagccc gtcgtcatgg
     3841 gtgcccgctc ggcgctgctg ctgcccttca gtcggctgga actcatcatt gtggacgaag
     3901 agcacgatcc ctccttcaaa caaaacgacc ccgcgccgcg ctacaacggc cgcgattccg
     3961 ccatttatct ggcccaactg cacggcgcca aagtgctgct cggcaccgcc acgccctcgg
     4021 tggagtctta ccacaatgcc ctgagcggca aatacggctt ggtggaaatg accgaacgct
     4081 tcggcggggt acaaatgccc gaaattgtac tggctgatgc ccgccaggaa atgaagcagc
     4141 gccagatgca gtcgcatttc accacacagt tgctcaacga actcaaagcc gccatcgagc
     4201 gcggcgagca ggccatcctc ttccaaaacc gccggggcta cgcgcccacc taccgctgct
     4261 tctcctnnnn nnnnnnggct ggcactccga gtgcatccac tgcgacgtga gccttactta
     4321 ccacaaattc agccacttac tcaagtgtca ctactgcggc taccagagca aactgccgcc
     4381 cgaatgcccg gcctgcggca atcgccaact cacgctctac ggcttcggca ccgaaaaaat
     4441 agaggacgaa ctcaaaattt acctccccga tgcccgcatt gcccgtctgg atttagacac
     4501 cgcccgcacc cgcaatgccc acgccgccat catcaacgac ttcgaggagc gccgcttcga
     4561 catcctcgtg ggcacacaaa tggtgaccaa gggcctcgat tttgaaaacg taggcatcgt
     4621 gggtgtcctc agcgccgacc agttgttgca gttccccgac ttccgctccg ccgaacgcgg
     4681 cttccaactc atggtgcagg tggcgggccg ggccgggcgc aaaaacaagc gcggcaaagt
     4741 catcatacag gccatgaaca cggcgcaccc ggtattgcag gaagtcattg ccaacgatta
     4801 caaaggcttt tttcagcgcg aaatcgccga gcgccgcgcc ttccgctacc cgcccttcac
     4861 gcgcctcatc cgcatcactc tcaagcacaa agcccccgaa gtgctcaacg ccggcgctga
     4921 aattttcacc aaagcgctcc accaaagcct cggcgactgg gtgaccggcc cggcagcgcc
     4981 ctacatctcc agagtgcgcg gcctgtacct gctcgactac ctcatcaaaa tggagcgcga
     5041 cccgcgcaaa atggaatttg ccaaatccag catagacacc gccacgcata ttttgcagtc
     5101 cacagagggt ttcagcggag tccgcgtggt agtggatgtg gacccggggt aaaaaccgaa
     5161 aaccaatccc tatctttgcc ccccaaaatc tttccgtaca tgacagccga tacgcagcgc
     5221 gccatagcct acgagcgggg cgccctctcc aacgaccaat tgatacaact ctacgagcgc
     5281 ctcctgtttc cgcgcatggt agaagaaaaa atgctcattc tcctgcgcca gggcaaaatc
     5341 agcaagtggt tttcgggcat cgggcaggaa gccatagccg tgggcgtcac tgccgccttg
     5401 ctgcccgacg aaatgctctt cacgctgcac cgcaacctcg gcgtgttcac cgtgcgcgac
     5461 atcccgctga gccgcctgtt ttgtcagtgg cagggcaagg attccggttt taccaaaggc
     5521 cgcgaccgct ccttccactt cggcgcctta gagtatggca ttgtgggcat gatctcgcac
     5581 ctcggaccgc aactctcgct ggcgggcggc gtggcgctgg cccacaaact gtcgggcgaa
     5641 ggccgcgtat ccgtagcgtt ttcaggcgag ggcggtacca gcgagggcga gttccacgag
     5701 gcgctcaata ccgctgccgt gtggcaactg cccgtcatct ttgtggtgga aaacaacggc
     5761 tacggcctct ctacgcccac taccgagcaa taccgctgcg aaaacatagc cgaccgcgcc
     5821 ataggctacg gcatgcgctc ctgcatcatt gacggcaata atattctgga ggtgtataac
     5881 accgtgtcgc aattggccgc cgaactacgc gagcaccccg aacccgtgct gctcgaatgt
     5941 cgcaccttcc ggatgcgcgg gcacgaggag gcctccggta ccaaatatgt acccaaagaa
     6001 ctgatggaac actgggcgct gaaagacccg ctggcgaact acgagcagtt tttgctggcc
     6061 gaaaatgtac tctccgaagc cgacgtgaag gccgttcgca agcgtttcaa agaccacatc
     6121 gaagcggaat tgaacattgc ttatgccgaa ggcgacgtga cctctaccga ggccgatgaa
     6181 ctggccgatg tgtatgcgcc ctgggagcag gaactgacgc agcccgtggg gacagcagag
     6241 gacaagcgct tggtggacgc catttcggac ggcctgcgcg aggccatgcg ccgccacgac
     6301 aagctcgtcc tcatgggcca ggacatagcc gactacggcg gggtgtttaa aataacggac
     6361 ggctttgtga aggaattcgg caaagaccgc gtgcgaaata cgcccatctg cgagagcgcc
     6421 attnnnnnnn nnnggcggcc attggcctga gcctcaaggg ctacaaggca atggtggaaa
     6481 tgcagttcgc cgattttgtg agctgcgcca tcacccagat catgaacaac gcagccaaac
     6541 tgcactaccg ctgggggcag cccgccgaca tcgtcatccg tatgccgacg ggcgcgggta
     6601 ccggcgcggg gccgtttcac tcgcagagca acgaagcttg gtttgcgcac acgcccggcc
     6661 tcaaagtgct gtacccctcc aatccgtacg atgcgaaggg catgttgctg gccgcttttg
     6721 aagaccccaa tccggtgatg tacttcgagc acagagcctt gtaccgcagc atttcggggc
     6781 cggtgccaca aggctactac accgtggaga taggcaaagc cgccaccgtg cgcgagggcc
     6841 gcgagttatc ggtcatcacc tacggcatgg gcgtgcattg ggcgctcaag gcagtggaag
     6901 cgctgggcat agacgccgag atcatagacc tgcgcaccct gttgcccctc gatttttcgg
     6961 ccatagacgc cacggtgaaa aagaccaaca aagtgctggt actgcacgaa gacacgatgt
     7021 ttggcggcct tggcggcgaa atatcggcct acatcaccga gcacttgttc gagcatttgg
     7081 atgcgccggt gctgcgggtc gcgtcgttgg atacgccgat acctttttcg ccggccatcg
     7141 agcgcatatt cctgcccgaa gagcggctga gaaagcagtt ggagcggctg aggggatatt
     7201 gagagtcaaa tagtggggcg tttttattat gccattttga aacaatgtaa ccggaaaacc
     7261 ttgttgtgaa agctggaaat aaattagcat tgaaatggaa ttaatccaca agtatcagaa
     7321 ggcactgtat aaagcatgtt ctgcgcacaa cgtggaagag ctatatacat tcggctctat
     7381 cttgacggac aagttcaatg ctgaaagcga tattgatttc attgttttga ttcgatcaga
     7441 agacccgatt gagtatgctg aaaattattt tgaactgaaa tttgaactgg aaaatatttt
     7501 caatagaaaa attgacctgt tggagcaaaa ggcgatcagg aacaagaggt tcgaagcatt
     7561 gatcaaccgg caaaaagtac tcgtttatgc aagaggaaat cagggtctgg cttgaggata
     7621 ttgaaaaggc ggtcggtgaa atcaatgggt ttctgcctga gccgagaaac ttcattgact
     7681 ttgtgaaaga ccttaaaacc aaacgggctg tggagcgaaa tatcgagata attggcgaag
     7741 caatgagccg gattttgaat atgcaacccg acattcccat cagcaattcc aggaaaattg
     7801 tagatactcg gaacagaatc attcacgggt acgactctgt ctcggatgat gtgatttggt
     7861 tgatcgtatc gagatatttg ccaattcttg aagccgagat taaagatatc caaagaggaa
     7921 attgaaaaga caagccgaca ataattccat gacgatagca agttcagagt tgcgcaagcc
     7981 ctgatactac cagcatcata ccaccaccaa tgaaagtaac cgactatttc gacaaggcca
     8041 ccggccaaac actcatctcc ttcgaggtgc tgccacctcc taaaggcgga ggtatgcagg
     8101 ccattttcag cgtattagac ccgctgatgg agttcaaacc gcccttcatt gatgtgacct
     8161 accaccgcga agacttcgtg tatgtggagc gtcccggggg gtattttgaa aaaatttcca
     8221 tccgaaagcg gcccggcacc gtaggtattt gcgccgccat tgtgcatcgc tacggggtgg
     8281 acgccgtgcc gcacctcatt tgcggaggct ttaccaaaga agataccgag aacgcgctca
     8341 ttgatctcaa ttttctgaat atcaacaacg tattggcttt gcggggcgat gcgcgcaagt
     8401 tcgacggcag gtttgtgccc gaagagcagg gccatgctta tgcccttgat ttggtgaaac
     8461 aaattgccga catgaacgcc ggcaggtatt tggacgaaaa catcgaaaac ggcgaacgca
     8521 ccgatttttg catcggcatt gcgggctatc ccgaaaagca tttcgaggcg cccaacatgg
     8581 aactcgatct ggagcacacc aaggccaaag tggacgccgg cgccaactat atcgtcactc
     8641 agatgttctt cgacaaccaa aaatatttcg agtatgtgca gcgctgccgg gacatcggca
     8701 tccaagtacc catcgtgccg ggcctcaagc ccatcaccaa agaaggccag ctcaacgcca
     8761 tacctcgcaa ttttttcata gatttgccgc acgaattggt ttcgtccgtt cagcaggcaa
     8821 aaaatgccga agccaaacgg caggccggca tcgaatggtg catcgctcaa agcaaagaac
     8881 tcaaagccgc cggagtacct tgcctgcatt actacaccat gggcgatgcc aaaaccaccc
     8941 ggcaaattgc cgaggctgta ttttaagcgc gcaatagagc gacttttttc gctgatttgc
     9001 cgcaaaacat tctgacaatg agtaacatag tagccatcgt aggccgccct aacgtgggca
     9061 aatccacttt tttcaatcgc ctcatcggcg aacgccaggc catcattgac gacatgcccg
     9121 gcgttacccg cgaccgccaa tacggcgcct cttactggaa cggcaaaaac tttaccgtaa
     9181 tagataccgg cggttttgta gaacattccg acgacgtgtt cgaggccgcc atccgctcgc
     9241 aggtgcgcat cgccatcgaa gaagcatccg tcatcgtctt tatggtggac gtcaccaccg
     9301 gcatcactga tttggacgaa gacgtggccg gcctcctgcg ccgcaccgac aaaccggtgt
     9361 atctggccgt caacaaagtg gacaatgccg aacggttcat ggaagccaac gagttttgga
     9421 gcctcggcat cgaaaatacc ttcttcctct cctcgctcac cggcagcggc agcggcgaac
     9481 ttttggacgc agtggtagat cactttgagg aggaagaacg cacggaaagc gcgctgcccc
     9541 gcattgccat catgggccag cccaacgtgg gcaaaagctc gctgaccaat gccctgctcg
     9601 gcgaagaccg caacatcgtc accgaagtgg ccggcaccac ccgcgattct atccacaccc
     9661 attattccaa gttcggcaaa gagtttttgc tcatagacac tgccggcatt cgcaaaaaag
     9721 ccaaggtaca cgaaaacata gagttctact ccgtcatgcg tgccattcgg gctatggagg
     9781 aatcggatgt ttgtatctta atgattgatg ctcagacagg tatggaaatg caggacatca
     9841 gcattttcag gctggcgcag cgccgcaaca aaggcatcgt cattttggtc aacaaatggg
     9901 acctggtgga agacaaagaa accaacactg cccgcgattt cgagcgcgcc atacaccagc
     9961 gcattgcgcc cttcaccgac gtgccgatca tcttcgtatc cgccaccgaa aaagtgcgca
    10021 ttttcaaagc aatggaggcc gctctggagg tgtttgagaa ccgcagccgc aagatcaaaa
    10081 cctccgaact caacgaggtg atgctcgaag ccatccagca ttatccgccg ccggcgtatc
    10141 ggggccgcct catcaagatc aaatatgtga cgcagttgcc gcccgcttat ccggcctttg
    10201 ccttcttctg caatcacnnn nnnnnnnnnn nnnnnccggg aaccgtaccg caactatctg
    10261 gaaaaccagt tgcgcaagca cttcaagttc accggttcgc ccatcagcat ctttttcagg
    10321 gagaagtaat cagcagccat gccgtttttg tcaacgccca ttaaaggctt aatcatcttc
    10381 gaaccgcaag tgtgggaaga tcatcgcggc tattttttcg agagctacaa tcgccgaacc
    10441 ttcgaggctg ccggcatctc tgccgatttt gtgcaagaca atcaggcgcg ttccacttat
    10501 ggcgtcctgc gcggtctgca cttccagaaa ccgccttttg agcaggccaa attggtgcgc
    10561 gtcaccgagg gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnna gaactgagcg ccgaaaacaa acgccaactc tttgtgccgc
    10681 gcggttttgc acatggttat gtggtactct cgccgacggc tgagttttgt tacaaatgcg
    10741 ataacttcta ctccaaagac cacgatgcag gcattcgttt tgacgacccc gccctgcaaa
    10801 tcgaatggcc gatcaagctc caggacgctg tgctttccga gaaggacctg gcgttgccgt
    10861 tttatttata atttggcatt tgaaaattat taacttgctt ctgtggtgta tatcctctat
    10921 ctttgcggat aagatattaa ataatcgctt ttacttcaaa atatttgaca atggactacc
    10981 tgtttcttgt tctaacaatt cctgcctata tacttgctgt tgttttgtgg aaagcaagaa
    11041 tccgattgaa aaggattggt gagtataagt cctttttgtt tgtggcaata aatgcattcc
    11101 tgggaatttt gggagcacag ataggctctt ttcaagcact ttttcttgaa aagaagtttc
    11161 aaattgaaaa cagttacggc ctttctttgc tcatcatgac aggtgtggtg gccttgcttt
    11221 atttttcatt tgtatattca aaaagagaag tcgaggcatg aggcattccc ttcgtccggt
    11281 agattctgtt tttgcccggc gtattttgag agatgctccg ttatccccgc tccgagctat
    11341 gacgacaggc ttggtttcgg atggtaaagg gttcgattcc tgattccggt aggaacagga
    11401 ctttattgca ggtttttcta cgcattcgta ctcacatctt tacaaatttc aatccggcac
    11461 tgatggcggc gcctcgtaag cgcgccgtgt aaacgcctga aggcaaagct gctatatctg
    11521 cctgaaaact gatgcgttgg ttatttttct ccaaaggaaa acgaagcaat gactgcccct
    11581 gcgaattgta gatttcgagg taaaatatgc cgatccattc gtgttccagt tcaaaatgta
    11641 accattccga tgcagggtta gggaagagtt ttattttgat actgatgtcc tgaacagcag
    11701 tagcagcatt caccattcct accacgaaat tgaaaacacg cgtacaacct ccggcatccg
    11761 ttacagaaag agcgtatgct ccggcgggaa tgttttcaag cctgtcgttc gtagctccat
    11821 tgctccatcg aagctcataa ggggcgcgac ctccctgaac ggacgtaata actatgctgc
    11881 caccggagct attgccggcc ggtgttattt ccgcagttgt tgaaagactg tcgggttcct
    11941 ccatgagtat ggcagcctgt cctgtacagc ccaaaccgtt ggtgatttca accgaatatt
    12001 gcccggctcc caattggttt atatcgggtg tttgcgctcc ggtattccac acaaaagatt
    12061 gaggcagggc tgtataaaca gggcttgcaa caacacggcc attagcatct ccccggcaga
    12121 tgatgggcga tacagtgacg gctacgggca gcgatccggc tatgggcata tcaaaaacta
    12181 aagtcgtatc gcagccatct gcatctgtta tcgtgaggtc atacatgccc ggaggcagtt
    12241 gttgcaggat tggttccatt gcgccattcg accattgaaa ctccagagga cctcgtgctg
    12301 tatcgggcag aatggcaatg ctgcccaatg tacccgtagc gcagttgacg ggaatgatat
    12361 gctgctcgga aatgctgatg ccggggaggg ccggcaattc gagtgcgccc atgtccacta
    12421 taccgtttat aacgcgaaga cctccggccg catccagcgt gtcgctgctg ccgagccagg
    12481 cattgctgcc cgcattgacg ggagggctgc aggggcgcaa tgaaaaatcg ccggactcta
    12541 cattctgaaa gggatgaccc gatgcaaaaa tattgtcgct gcattctaag ccgccgaggt
    12601 attgatagat tggcggggta ttggggtcaa taatgatttc aacgggaaat gtctcaaaaa
    12661 tctgagtaca cgaatccgcc caaaataaac aattttgaat actcgcccaa gtattacgtc
    12721 caaatatgta tggtcggtca acagtatcag aataagcgaa aatagagttt cttaatagta
    12781 gtgtatccgg gtagtttatt gatattactt gagagtttcg tttgttgatg aatggagcaa
    12841 tgatcgtatc tgacaggtaa aaagtagaat tgataatttc
//
LOCUS       DAORLF010000071        12785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2618_length_12785_cov_1.868185, whole genome shotgun sequence.
ACCESSION   DAORLF010000071 DAORLF010000000
VERSION     DAORLF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12785
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_12785_cov_1.868185"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK198_08015"
     CDS             <1..484
                     /locus_tag="PK198_08015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF38719.1"
                     /translation="MSLTWQEETPELPVLSGSVTKIVLVRHAEKVLDGSEDPSLTEAG
                     QQRAERLKFFLTDMKIDKFYSTKYKRNVLTLMPLAKSRGLNIDIYEAWDKDFPAWVLK
                     ENAGKNIVISAHSNTAPILVNTWLGKEQFAQLPDEEYGKIWVLTFQNNQLTDCSVFNY
                     "
     gene            489..3653
                     /locus_tag="PK198_08020"
     CDS             489..3653
                     /locus_tag="PK198_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF38720.1"
                     /translation="MTKHFFLLILLGLSFNTFAQTPKPATPPTDLNQFFHPAKWRNIG
                     PFRGGRSVAVSGVVGDPATYYMGTTGGGLWKTDDMGIIWSNISDGFFQTGSVGAVAVS
                     ESDPNVVYVGMGEHAVRGVMTHHGDGIYKSTDAGKTWKKLGLDQSQHIARIIIHPSNP
                     DIAWAAVQGALYSSSKQRGIYKTTDGGATWKSVLFVDEKTGCGEISIDHNNPRILYAA
                     MWEYGRLPWKVISGGPGSGLYKSADGGESWQKMETGLPKEMGKMSVAVCRSNSEKVYA
                     LIESDSEKEAGGLFVSGNGGKNWSRVSDDHRLVQRAWYYIEMFPDPKNENTVYVLSAP
                     MLRSEDGGKTWEVMSGPHGDYHDMWINPRNSKNFVIADDGGAGITFNYGKSWSSQANM
                     PTAQLYRINVDNQFPYRIYAGQQDNTSVVIAHRELGGYGISTASWEASAGGESAFLAF
                     DPDNPRYVLGGSYLGTIEVLDTKAKAGTNIMAAPIQYLARDAKDMKYRYNWNAPIIWS
                     RHEPNTYYHAAQVLLKTQDMGKTWKEVSPDLTRNEKEKQGKGGGPYTNEAVGAENYGT
                     ISYVVESPHEKGVIWTGSDDGLVQLTTDGGQNWKNVTPAGLQECLINAIEVSPHDKAT
                     AYIATTRYKFNDHAPGIYKTTDYGKTWTKITNGLPANAFTRVVREDEVRRDLLIAGTE
                     IGMFLSWDGGKNWQPFQLNLPITPITDLKIHKGDLIAATSGRSFWIFDDMNLLRSFKK
                     DQSGFFAIAPEPAYLANGGSPLNSSDENFKGMGSTEGVNPANGVVLYYHLPEEIKDGE
                     LKMEIRDSEGNLVRTFSSKPDASFQEWDGGPSAEPVLSAKKGLNRFVWNMRYPTMAGI
                     PNVYYEASFSGHKASPGKYVVKLMQGDKTVETTAEILSNPLYSVSADDYQNYHRFMQA
                     AEAELHQMHLMVNELYAKKTQLEELLAALPVGDAFKMLKTDGEALAKKITAWDEEMVQ
                     RKSKAYDDVENFPNKFTAEYIFLINMTESDLPRVNQPSLDRKAELDAQWAGLKMRGRE
                     LLDKDLPALNEKLWKAGIGGVWKK"
     assembly_gap    3822..3836
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3837..>4672)
                     /locus_tag="PK198_08025"
     CDS             complement(<3837..>4672)
                     /locus_tag="PK198_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38721.1"
                     /translation="DESGLQDMNDPQTYVSLILNNDISPTTCNDLNADEDISVYDAAL
                     LSSCLNYGRNHPHVGQGLHDHCTFPSGADNTLDTVALSIIAANFDEQYIDIGIRNPSS
                     YVTAYQFKMSGITIQQVENLVNPAVYPIIPRNNISEGMVIGISYQDSAVDKSNVVQPL
                     CRIRYSGIEPGLVCIDRIIDIVDRAPHRVANRIEDGCVEYTVSSVNGPVANNMAVSVQ
                     PNPFDQSTLLRFANPEGGAYRLELMDAHGRLVRRYTDISGGEHRIERGDLSSGLYFFR
                     LY"
     assembly_gap    4673..4712
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4713..6189)
                     /locus_tag="PK198_08030"
     CDS             complement(<4713..6189)
                     /locus_tag="PK198_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRF38722.1"
                     /translation="MHKIYLMLAALSCLTALNAQPPCPAATPVSIVVTIRTDAYGYET
                     GWDVTGANGTVYHSVGFNTYANHTVYQTQVCVPQDECIKIRIRDSYGDGIFSPGYYVV
                     QANGDTIASGRVFRYEVPIHFNCPAGLICSTAIPAEAGGVYQAPFDDTWFSFTPDSTG
                     MYLISTCDSNTCDTKIWVYDACSGITIGEDNPGTIFYDDDEKGCGNQAAIRGHFAAGT
                     TYYIRIGDDMDGCTDTIIWMLSYLGPVVGCTDPTSCTFNPLATVDDGSCLPQGHPDCP
                     PGPDLVMRGDIMRSTLNLTTINSTDPCLIQEGCLKGFGQRDIIRFTTRIDNIGPKDYF
                     IGVPSQTNNQFTWDNCHNHFHYAGYAEYLLFDQNGAVTPAGFKNGFCVLDLGCTTGTA
                     KYGCGNMGISAGCHDTYSAGLSCQWIDVTDIPDGRYTFVTRVNWDNAPDALGQVESDT
                     LNNWAQVCIVISRASGQLTMTVDNNCPPFVDCAGTPYGSAQP"
     gene            complement(6276..7784)
                     /locus_tag="PK198_08035"
     CDS             complement(6276..7784)
                     /locus_tag="PK198_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF38723.1"
                     /translation="MASKDDFIKAAQPALDRLLKAYPNPKEDLCISDVVDAEGHQYVN
                     LVQEGGGVLGVALLGYTYVLEQMGIRFMKMAGTSAGAINTMMLAALGEKHEMKSLKIL
                     ELLTNKNLFDFVDGGKLVRTLIRNLITSTGFFKRIMVLLVVVAALFITSLFATLFLAG
                     RMVLDAPLIVTATVLMICIALIAYIWFWVSRRQKDFSQAEFGLNPGRDFENWVTGILK
                     DNGIHHLGDLRRHIEKTPPGGFRLRRSDNPEDLSDLNSPSMEKFLVLVTSDITNQIKV
                     EFPRMWNLYWAKEEDVNPAGFVRASMSVPVFFRPFASPEIEPEKFEGRWNQLVGPESA
                     SRISPKAKFVDGGIISNFPINVFYNPSVDAPRMPTFGIMLEDADPPQPAQGFKNFFGY
                     AGAMVSTLRYHYDKEFLIKHADFKKTIGEIDVRGVNWLNFNLTDDEKIDLFRRGAMAA
                     ADFFLGKESAKTPAEMPTIKYDDTTKSEVPQGGFDWEYYKRYRKVRKRRLGY"
     gene            complement(7930..>8845)
                     /locus_tag="PK198_08040"
     CDS             complement(7930..>8845)
                     /locus_tag="PK198_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF38724.1"
                     /translation="GDLGTVFATGARARKGNARDKDAEVLPQLTMSAEHYNRIFRLLR
                     RGMPVKLAMELSAEYTNPNGMSRNIIAEIPGTDLKDEVVIFGAHIDSWHLATGATDNA
                     AGSAVMIEAARILFETIKESGVQPRRTLRLALWTGEEQGLLGSRAYVRDHYAELDSMG
                     SVISYKPAQEKVAGYYNLDNGTGKVRGIYLQGNPAVQQIFRAWLEPFKDLDANTITLS
                     NTGGTDHQSFDRVGIPGFQFIQETIAYSTRTHHSNMDNFDHLIADDLKQAATVIASMV
                     WHTSQRTEKLPRKPKEEEAPRSRAQGGQ"
     assembly_gap    8846..8867
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8868..9515)
                     /locus_tag="PK198_08045"
     CDS             complement(<8868..9515)
                     /locus_tag="PK198_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HRF38725.1"
                     /translation="MKTLRILFLLAITGLSFTAFAQDKADENVNKILRKHGLEQSQVM
                     DIAAMLTDVHGPRLTGSPKLDDATRWTVDELKKWGLSNVHTEDWGPFGRGWELKGFHL
                     SCEAPTYYPIIAYPKAWSPSVKGRITGEVVYLEAGSEEDLAAYKGKLKGKFVLLDTLR
                     AIEAPFDAEARRYNSEDLLKMANAPMPTPFGFRNWGSGQRFTTQLWDFLYEEKPAA"
     gene            9764..>10493
                     /locus_tag="PK198_08050"
     CDS             9764..>10493
                     /locus_tag="PK198_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRF38726.1"
                     /translation="MPRMSDTMTEGNIVGWLKKEGDKVEPGDTLAEVETDKATMELDS
                     PAEGVILHIAVKEGAVPINAVIAVIGKQGEDWQTAIAAAGGASNGSVAAAPAPEAPAA
                     QAPATPAAPAPAPSAEESRTKASPLAKSMAKETGVDLAQVPGSGDQGRIVKRDVEAFI
                     ESQKSAPAPAPAAAPAAPATQAPAVNPFYFGGGEGNFEERPVSMMRKTIARRLGESKF
                     TAPHFYLTMEINMDKTVQMRERLNE"
     assembly_gap    10494..10503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10504..11033
                     /locus_tag="PK198_08055"
     CDS             <10504..11033
                     /locus_tag="PK198_08055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRF38727.1"
                     /translation="PVKISFNDFVIKACTAALRQHPAINSSWLGDKIRTNHDIHIGVA
                     VAVDEGLLVPVIRHADMKTMSQINTEVKAMALKAKDRKLQPEEMTGNTFTISNLGMFD
                     IEEFTAIINPPDSCILAVGSIIQKPIVKNGEIVVGNMMRVTLSCDHRVVDGATGAKFL
                     QTLKGVLEDPIRLLV"
     gene            11142..>11676
                     /locus_tag="PK198_08060"
     CDS             11142..>11676
                     /locus_tag="PK198_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRF38728.1"
                     /translation="MKKTLVLGASPDPSRYSYAAVHQLRDKGHEVVAVGKRPGAVADV
                     TIVQEFPQLEDIHTVTLYLAPAHQAPMYDYILQLKPQRIIFNPGTENRELAQLAKDRN
                     GAGLHAGDVVGGVVLRENLPRKFISHVHAHTCLHPPRTRTAFRFVETTAFFLPTKRVH
                     QAQASLERKRGSVFQRGH"
     assembly_gap    11677..11788
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11789..12350
                     /locus_tag="PK198_08065"
     CDS             <11789..12350
                     /locus_tag="PK198_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRF38729.1"
                     /translation="KTLVFSKRLARLSAQLMGASGARLYHDQALFKEPGGGITPWHAD
                     QHYWPLNTDKTITAWMPLQATPLEMGPLEFSPGSQRILAGRELSIGDESEKLIGEKLK
                     INDFPHVVEGFECGEISFHSGWVFHRAGANQSREMRKVMTVIYMDKETTLMEPKNSGQ
                     LNDWKTWCPGAEVGQIINTPLNPVLY"
     gene            complement(12363..>12785)
                     /locus_tag="PK198_08070"
     CDS             complement(12363..>12785)
                     /locus_tag="PK198_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38730.1"
                     /translation="TLFAFIFIGSFFSAIGQPTEVRQRRDKITIYYRNTTEASIDVPV
                     LKFRTDLEGKYMYKEYYQINNDTLHLLISQEVSSNLIPIINREGGGTSSARLLYNDFE
                     LQPGNKYKSSIRIRTLPPVSIVFMNNTFIPMRGKRRKQ"
BASE COUNT         3068 a         3346 c         3360 g         2812 t
ORIGIN      
        1 catgagcctc acctggcagg aagaaacgcc cgaattgcct gtactttccg gcagcgtcac
       61 caaaattgtg ctggtacgac atgccgaaaa agtgctcgac ggctccgagg atccatccct
      121 caccgaagcc ggccaacaac gcgccgaacg cctgaagttc tttttgacgg atatgaaaat
      181 tgacaaattt tactccacca aatacaagcg caatgtattg acattaatgc ctttagcaaa
      241 atcaagaggg ctgaatattg atatttacga ggcttgggac aaagattttc ccgcctgggt
      301 tttgaaagaa aacgccggta aaaacatcgt catcagcgcc cattccaata cggcgcccat
      361 cttagtgaat acctggctgg gaaaagagca gttcgctcag ctccccgatg aggaatacgg
      421 caaaatctgg gtgctcacgt tccaaaacaa ccaactgacc gattgctcgg tgtttaatta
      481 ttgaaaccat gaccaagcac ttctttctcc tcatcctctt gggcttatcg tttaacactt
      541 ttgcccaaac acccaaaccg gccacaccgc cgaccgacct caaccaattc ttccatcctg
      601 ctaaatggcg caatatcggg ccgttccggg gcgggcgttc ggtagcggtt tccggcgtgg
      661 tgggcgatcc tgccacctat tacatgggca ccaccggcgg cggtctctgg aaaaccgatg
      721 atatgggcat catctggagc aatatctccg acgggttttt ccaaacgggt tcggtaggcg
      781 cagtagccgt atctgaaagc gaccccaacg tagtgtatgt gggcatgggc gaacatgcag
      841 tgcgcggcgt gatgacccat catggcgacg gtatttacaa gtcaaccgat gccggcaaaa
      901 cctggaaaaa attgggtttg gaccaaagtc agcacattgc acgcatcatc atccatccgt
      961 ccaaccccga cattgcctgg gcggcagtgc agggcgcttt gtacagttcg tcgaagcagc
     1021 gcggtattta caaaaccacc gacggcggcg ccacttggaa aagcgtcctt tttgtggatg
     1081 agaaaaccgg ttgcggcgaa atttctattg accacaacaa cccgcgcatc ctctatgccg
     1141 cgatgtggga atatggccgc ctgccctgga aagtcatcag cggcgggccg ggcagcggtt
     1201 tgtacaaatc ggcggacggc ggcgaaagct ggcagaaaat ggaaacagga ttgcccaaag
     1261 aaatgggtaa gatgtcggtg gcagtatgcc gctcaaattc tgaaaaagta tatgccctca
     1321 tcgagagcga ttctgaaaaa gaggccggcg gcttgttcgt ttccggcaac ggcggtaaaa
     1381 actggtcgcg ggtgagcgac gaccaccgtc tcgtgcagcg cgcctggtat tacatcgaaa
     1441 tgtttcccga ccccaagaat gagaacaccg tgtatgtgct cagcgcgccc atgttgcgct
     1501 ccgaagacgg cggcaaaacc tgggaagtga tgtccggccc gcatggagac taccacgata
     1561 tgtggatcaa tccgcgcaat tccaaaaact tcgtcattgc cgatgacggc ggcgcgggca
     1621 ttaccttcaa ttacggcaaa agctggagtt cgcaggccaa catgcccacc gcgcagttgt
     1681 accgcatcaa cgtggacaac cagtttccgt accgcattta cgccggtcag caagacaata
     1741 cctcggtcgt cattgcgcac cgggaattgg gcggctatgg catctccacg gcctcttggg
     1801 aagcctctgc cggcggagaa agcgctttcc tcgccttcga tcccgacaac ccgcgctatg
     1861 tcctcggcgg cagctacctc ggcaccatcg aagtgctcga taccaaggcc aaagccggta
     1921 ccaacatcat ggcggcgccc attcaatacc tcgcccgcga cgccaaagac atgaaatacc
     1981 gctacaactg gaacgcgccc atcatctggt cccggcacga acccaacacc tactaccacg
     2041 cggctcaggt gctgctgaaa acgcaagaca tgggcaaaac ttggaaggaa gtgtcgcccg
     2101 atctgacccg caatgaaaag gaaaagcagg gcaaaggcgg cggcccttac accaacgaag
     2161 ccgtgggcgc tgaaaactac ggcaccatca gttatgtggt ggagtcgccg cacgaaaaag
     2221 gcgtgatctg gacgggcagc gacgacggct tggtacaact caccaccgac ggcggccaaa
     2281 actggaaaaa cgtaacgccg gcgggccttc aggaatgtct catcaatgcc attgaggtgt
     2341 cgccgcacga taaagcaacg gcttacattg ccaccacgcg ctacaaattc aatgaccatg
     2401 cgccaggtat ttacaaaacc accgactacg gcaaaacctg gaccaaaatc accaacggct
     2461 tgcctgccaa tgccttcacg cgggtggtgc gcgaggacga agtgcgccgg gacctgctca
     2521 tcgccggcac cgagatcggc atgtttcttt cctgggatgg gggtaaaaac tggcagccgt
     2581 tccaactcaa tctgcccatc acgcccatca ccgacctcaa aattcacaaa ggagacctca
     2641 tagccgccac ttccggtcgc tcgttctgga ttttcgatga tatgaacctc ctgcgttcat
     2701 ttaaaaaaga ccagagcgga ttttttgcca tcgcccccga accggcttac ttggccaatg
     2761 gcggcagccc gctcaacagc agcgacgaga atttcaaagg catgggcagc accgagggag
     2821 tcaatccggc caacggcgtg gtgctgtatt accacctgcc ggaggagatc aaagacggcg
     2881 agttgaaaat ggaaatccga gattcggaag gcaatttagt gagaacattc agttcaaaac
     2941 cggacgcctc gtttcaggag tgggacggcg ggccttcggc agaaccggtg ttgagcgcca
     3001 aaaaaggtct gaaccgcttt gtctggaaca tgcgctatcc tacgatggcg ggcatcccga
     3061 atgtttatta cgaagcgagt ttttcggggc acaaagcctc tccgggcaaa tacgttgtga
     3121 aactgatgca gggtgataaa acggtggaaa caacggctga aatcctgtcc aatccgctgt
     3181 attccgtcag tgccgacgat taccaaaact accatcgctt catgcaggcg gcggaggcag
     3241 aattgcacca aatgcacctg atggtcaatg aattgtatgc caaaaaaacg caattggaag
     3301 aactgttggc cgctctgccg gtcggggatg ctttcaaaat gctgaaaacg gatggtgaag
     3361 cgttggccaa aaaaatcact gcctgggatg aagaaatggt gcaacgcaaa tcgaaagctt
     3421 acgacgacgt ggagaatttt cctaacaaat tcaccgccga atacatcttc ctcatcaaca
     3481 tgacggaaag cgatctgccc cgcgtgaacc agccttcatt ggatcgcaaa gcggagttgg
     3541 atgctcagtg ggccgggctg aaaatgcgtg gccgcgagtt gttggacaaa gacttgccgg
     3601 cgttgaatga aaaactgtgg aaggcgggca tcggcggggt ttggaaaaag tgatttgagt
     3661 gctaagttag ccctttaccg gcctaaaatg ttctactccg gcagcttgaa ccgcacgggc
     3721 aggttgaatc gttgaagggg cgactacgga cgatggccgg tcgccccttc actaaaccga
     3781 tggagatgcg cctacctcgc caccaatttc cccgtagcct gnnnnnnnnn nnnnnncgta
     3841 caatcggaag aaatacaatc cgctgctcag gtcgccgcgc tctatgcgat gctcgccgcc
     3901 gctgatgtct gtgtagcgcc gcaccaaacg accatgcgca tccatcagtt cgaggcgata
     3961 agcgccgcct tcgggattgg caaaacgcaa cagggtggac tgatcgaacg gattgggctg
     4021 cacagaaacc gccatattgt tggcaacagg cccgttgacg ctgctcaccg tgtattccac
     4081 gcagccgtct tcgatgcgat ttgccacgcg atgcggcgcg cgatccacga tgtctatgat
     4141 gcggtcaata caaaccaagc ccggttcgat gccggagtag cgaatgcggc acaatggctg
     4201 caccacatta ctcttgtcca ccgccgaatc ctgataagaa ataccgatga ccatgccctc
     4261 gctgatgttg ttgcgcggga tgatggggta aaccgccgga ttgactaagt tttctacctg
     4321 ctgaatcgtg atgccgctca ttttgaactg ataagccgtc acatagctgg aggggttacg
     4381 gatgccgatg tcaatgtatt gctcgtcaaa attggccgca atgatgctga gcgccaccgt
     4441 atccaatgtg ttgtcggcgc cgctggggaa ggtgcagtga tcgtgcagac cctgcccgac
     4501 gtgcgggtga ttgcgcccgt agttgagaca actggagagc agtgccgcat cataaaccga
     4561 tatgtcttca tcggcattga ggtcgttgca ggtggttggg ctgatgtcgt tgttcaaaat
     4621 gagcgagacg taggtttgcg ggtcgttcat gtcctgcaag ccgctttcgt ccnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgggctga gccgaaccgt agggcgtacc
     4741 tgcgcaatcc acaaaaggcg ggcagttgtt gtcaactgtc atggtgagct gcccggaagc
     4801 gcggctgatg acgatgcaga cctgcgccca gttgttgagc gtgtcagatt ctacctgccc
     4861 gagtgcatcg ggagcattgt cccagttgac gcgagttaca aaagtgtatc gcccgtcggg
     4921 gatgtcggtc acgtctatcc attggcagga aaggccggcg ctgtaagtgt cgtggcagcc
     4981 ggcagagatg cccatgttgc cgcaaccata ttttgcagtt ccggtagtac agcccagatc
     5041 gagtacacaa aagccgtttt tgaagccggc gggcgtcacg gcgccgtttt gatcaaacaa
     5101 cagatactcg gcataaccgg cgtagtgaaa gtggttgtgg cagttgtccc aggtaaactg
     5161 gttgttcgtt tgcgagggca cgccgatgaa gtagtctttg ggaccgatat tatcaatgcg
     5221 cgtggtgaag cgaatgatgt ctcgttggcc aaaacctttg aggcagcctt cctggataag
     5281 acaggggtcg gtggaattga tggtcgtcag gtttaaggta gagcgcataa tatctccacg
     5341 catcacgagg tctggtccgg gtgggcagtc gggatgcccc tgcggcaggc aagagccgtc
     5401 gtccacggtg gcgaggggat tgaatgtaca agatgttgga tcggtacatc ccaccacagg
     5461 gccgaggtaa gacagcatcc agatgatggt gtcggtacag ccgtccatat catcaccaat
     5521 gcgaatgtag taggtggtac cggcagcaaa atgccctcga atggccgcct gattgccgca
     5581 gcctttttca tcatcgtcat agaaaatagt acccggattg tcttcgccaa tagtgatgcc
     5641 gctgcaagcg tcatataccc atattttggt gtcgcaggta ttggagtcgc aggttgaaat
     5701 gaggtacatg ccggtagagt cgggtgtaaa agaaaaccaa gtatcgtcga aaggcgcctg
     5761 ataaacgccc ccagcttcgg caggaatggc cgtagagcaa atcaatcctg ccggacagtt
     5821 gaagtgtata ggcacttcat agcggaaaac tctgcccgat gcaatcgtat cgccattggc
     5881 ctgcaccaca taatagcccg gcgaaaaaat cccgtcgccg tagctgtcgc ggatgcgaat
     5941 tttgatgcac tcgtcctggg gtacgcaaac ctgtgtctga tatacggtat gattggcgta
     6001 ggtattgaac ccgacactgt ggtaaaccgt gccgttggcg ccggtcacat cccaaccggt
     6061 ttcgtagcca tacgcatccg tacggatggt cactacaatg gagacgggtg ttgccgcagg
     6121 gcaggggggt tgcgcattca gcgcggtcag gcagctcaat gccgccagca taaggtaaat
     6181 tttgtgcatg atagatagtt taaaattccg tgtttcggat tccatgtttc gggttgctca
     6241 ctcttcacac actcacccct cacccctgaa aaccgttaat atcccaatct acgctttctc
     6301 accttccggt aacgcttgta atattcccag tcgaagccgc cttgaggcac ttccgacttg
     6361 gttgtgtcat catactttat agttggcatc tcggcaggag ttttagcgct ttctttaccc
     6421 aaaaagaaat cggcagcagc cattgcgccc cgtcggaaga gatcaatttt ttcgtcgtcc
     6481 gtcagattga aattgagcca gttgacaccg cgcacgtcaa tttctccgat ggtttttttg
     6541 aagtcggcgt gtttgatcaa aaactcctta tcgtagtgat agcgcaaagt gctgaccatg
     6601 gcgccggcat atccaaaaaa atttttgaaa ccctgcgcag gctggggtgg gtcggcatct
     6661 tcgagcatga taccaaaggt aggcatacgc ggcgcgtcca cgctcggatt gtaaaatacg
     6721 ttgatcggga aattggaaat gatgccgccg tccacaaact tggctttggg cgaaatgcgg
     6781 gaggcgctct ccgggccgac caattgattc cagcggccct caaatttttc aggctctatt
     6841 tccggcgatg caaagggcct gaagaaaacc ggcaccgaca ttgaagcccg cacaaagcct
     6901 gccggattca cgtcttcttc tttggcccag tacaagttcc acattctcgg aaactccact
     6961 ttgatctggt tggtgatgtc ggatgtgacc aataccagaa atttttccat actcgggctg
     7021 ttgagatcgc taagatcctc cgggttatcg ctgcgccgga gccggaagcc gcccggaggc
     7081 gttttttcaa tgtggcggcg caggtcgccc aagtgatgga tgccgttgtc tttcaaaatt
     7141 ccggttaccc agttttcaaa atcgcggccc ggattgagac caaactcagc ctgcgaaaaa
     7201 tctttctgtc tgcggctgac ccaaaaccat atatatgcaa tcaaggcgat acaaatcatt
     7261 agtaccgtcg cagtcacaat aagcggtgca tctaaaacca tgcgaccggc cagaaataag
     7321 gtggcaaaaa gcgaggtgat aaacaatgca gcaacgacga caagcaagac catgatgcgc
     7381 ttgaaaaaac cggtggaggt gatgaggttc cttattaaag tgcgcactaa tttgccgccg
     7441 tccacaaaat cgaagaggtt tttattggtt aataactcca atattttcag cgatttcatc
     7501 tcgtgtttct cgcctaaagc cgccagcatc atggtgttga tggcgcccgc gctggtgccg
     7561 gccattttca taaaccggat gcccatttgt tccagcacat aggtataacc cagcaaggca
     7621 acccccaaca cgccgccgcc ctcctgcacc aggttgacat actgatgccc ctccgcatct
     7681 accacgtcgg aaatgcagag gtcttctttc ggattcgggt aagctttgag caatctgtct
     7741 aatgccggct gtgccgcttt gatgaaatca tctttggagg ccatgatgtg aaggtgtttt
     7801 ggttgcgtat tcaaatcaaa tagaaaatag ccatgaatta cgagcgatga actgccgaag
     7861 gcaacttacg gcaaagccga taaactgcga agcagcttac ggcaaagccg ataaactgcc
     7921 gaaggcaact tactgccctc cctgtgccct cgaacgcggc gcctcttcct ccttcggttt
     7981 gcgcggcagc ttctctgtgc gctgcgaggt atgccaaacc atagatgcaa tgacggtagc
     8041 ggcctgcttg aggtcatcgg cgatgaggtg gtcgaagttg tccatgttgg aatggtgcgt
     8101 acgggtagaa tatgcaatcg tctcctgaat gaactggaaa ccgggaatgc ccacgcggtc
     8161 gaacgactgg tggtcggtgc cgccggtatt ggacagtgtt atcgtattgg catccaagtc
     8221 tttaaacggt tcaagccatg cgcggaaaat ctgctgtact gccgggttgc cctgcaagta
     8281 gatgccgcgc actttgcctg tgccgttgtc caaattataa taaccggcta ctttttcctg
     8341 tgccggtttg tagctgatga cgctgcccat agaatcgagt tcggcgtagt gatcgcgcac
     8401 ataagcccgc gagccgagca agccttgttc ttcgccggtc caaagagcca gacggagtgt
     8461 gcggcgcggt tgtacgccgc tttctttgat ggtttcaaac aaaatgcggg cggcctctat
     8521 catcacggca gaaccggcgg cgttgtcggt ggcgccggta gccaagtgcc aggaatcaat
     8581 atgcgctccg aaaataacta cctcgtcttt gaggtcggtg ccaggaattt cggcaatgat
     8641 gttgcgcgac atcccattgg gattggtgta ttcggcgctc agttccatcg ccaatttgac
     8701 gggcattccc cggcgcagga gcctgaaaat gcggttgtaa tgttcggcgc tcatggtgag
     8761 ttggggcagt acttccgcgt ctttgtcgcg ggcgttacct ttacgtgcgc gggcgccggt
     8821 ggcaaacaca gtgccgaggt cgcctnnnnn nnnnnnnnnn nnnnnnnagc ggcgggtttt
     8881 tcttcgtaca ggaagtccca cagttgcgta gtaaagcgtt ggccgctgcc ccagttgcga
     8941 aaaccaaagg gcgtaggcat gggcgcattg gccattttca acaggtcctc ggaattgtaa
     9001 cggcgggcct cggcgtcaaa aggagcttcg atggcgcgga gggtgtccaa cagcacaaac
     9061 ttgcctttga gtttgccttt gtaggcggcc aggtcctctt ctgaacctgc ctctaaatac
     9121 accacctcgc cggtgatgcg cccttttacc gacggcgacc aggctttggg gtaggcgatg
     9181 atggggtaat atgtgggcgc ttcgcaactc aggtggaagc ctttcagttc ccagcccctg
     9241 ccgaacggcc cccagtcttc ggtatggacg ttgctcagtc cccatttttt gagttcgtcc
     9301 acagtccagc gcgtggcgtc gtccaatttg ggcgaacccg tgaggcgcgg gccgtgtacg
     9361 tcggtgagca tggcggcaat atccatcacc tggctttgct ccaagccatg tttgcggagg
     9421 attttattga cgttttcgtc ggccttgtct tgggcgaaag ccgtgaagct gaggccggta
     9481 atggccagaa ggaagagaat tcgtaaagtt ttcatggaca gttgagtgat gtttgaatgt
     9541 tgtaatcgca attcgaggcg caattttaca ccaataaaat tacttttcag gatttaaaaa
     9601 tcaaagaaat ggcaataacg catttcaata ttgcaaaaaa tggaattgtc ctgaaaaata
     9661 ctccgcgacg ctcccacaat ccgcaaaatg tcgcattttt gcgaaccgaa attggaacac
     9721 cttgttccaa aacccgtcat tgactgaaaa acaactgttt attatgccgc gaatgagcga
     9781 caccatgacc gaaggaaaca tcgtgggatg gctcaaaaaa gaaggcgaca aagtagagcc
     9841 gggcgatacc ctggctgaag tggaaaccga caaagcgacc atggaattgg acagccccgc
     9901 cgagggcgtc attcttcata ttgctgtcaa agaaggcgct gtgcccatca atgccgtcat
     9961 tgctgttatt ggtaaacaag gcgaagactg gcagaccgcc attgccgctg ccggcggtgc
    10021 ttccaatggc tcggttgccg ctgctcctgc acctgaagct cctgccgccc aggctccggc
    10081 aactcccgca gcacctgcac ctgctccctc cgcagaagaa agccgcacga aagcttctcc
    10141 tttggctaaa agtatggcca aagaaaccgg cgttgacctg gcccaagtgc ccggttccgg
    10201 cgatcagggc cgcatcgtga agcgcgatgt ggaggctttc atcgaatctc aaaaaagcgc
    10261 tccggcccct gcacccgctg ctgctccggc cgcgcccgcc acacaggcgc ccgccgtgaa
    10321 tccgttctat ttcggcggcg gcgaaggcaa tttcgaggag cgcccggtga gcatgatgcg
    10381 caaaaccatt gcccgccgtt tgggcgaaag caagttcacg gcgccgcact tctacctgac
    10441 catggaaatc aacatggaca aaacggtgca aatgcgcgaa cgcctcaacg aggnnnnnnn
    10501 nnncgccggt caaaatttct ttcaacgatt ttgtcatcaa agcctgcacg gcggccctgc
    10561 ggcagcatcc ggccatcaat tcttcctggt tgggcgacaa aatccgcacc aaccacgata
    10621 tccacatcgg ggtggcagta gctgttgacg aaggcctgct ggtgcccgtc atccgccatg
    10681 ccgacatgaa aaccatgtcg caaatcaata ccgaagtgaa ggcaatggcc ctcaaagcca
    10741 aagaccgcaa actccaaccg gaggaaatga cgggcaatac cttcactatt tccaacctcg
    10801 gcatgttcga catcgaagaa tttaccgcca tcatcaatcc gcccgattcc tgcatcctcg
    10861 ccgtgggttc catcatacag aaacctattg tgaaaaacgg cgaaattgtg gtgggcaaca
    10921 tgatgcgcgt caccctctcc tgcgatcacc gcgtagtgga cggcgctacc ggagccaaat
    10981 tcctgcaaac gctcaagggc gtgctggaag atcccatccg actgttggtg tagtaggtct
    11041 ttctttagct gcgagcttcg agccacgagt ttgctcccgt agagcgctcg tagctcgaag
    11101 ctcatagctc gcggctattc taaaaaatac caccttacaa aatgaaaaag acccttgtgc
    11161 tgggcgcatc gcccgatcct tcccgctatt cctacgccgc cgtacatcag ctccgcgaca
    11221 aggggcatga ggtcgtggca gtcggcaagc gtcccggcgc cgtggctgat gtgaccatcg
    11281 tgcaagaatt tcctcaactt gaagatattc ataccgttac gctgtatttg gccccggccc
    11341 atcaagcgcc tatgtacgac tacatcctgc aattgaaacc tcaacgcatc atcttcaatc
    11401 cgggcaccga aaacagggaa ctggcacaat tggccaaaga tcgaaacggt gcaggcctgc
    11461 acgctggtga tgttgtcggt ggggttgtat tgagggaaaa tttgccgcgt aaatttatca
    11521 gccatgtcca cgctcatacc tgcctccatc ctccacgaac tcgaacagcc tttcgatttg
    11581 tcgaaacaac agctttcttt ttaccaacaa aacgggtaca tcaagctcag gcaagtcttg
    11641 agcgaaagcg cggttcagtt tttcaacgag gccatcnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aaacgcttgt cttttccaag cggcttgccc
    11821 ggctttccgc ccaactgatg ggcgcttcgg gcgcaagatt gtaccacgat caagccttgt
    11881 tcaaagagcc gggcggaggc atcactccct ggcatgcaga ccagcactac tggccattga
    11941 ataccgacaa aaccatcacc gcctggatgc cgcttcaggc tactcctttg gagatggggc
    12001 cgcttgagtt cagccccggc agccagcgca ttctggccgg cagagaatta agcattggtg
    12061 acgagagtga aaaactaatt ggcgaaaagc tgaaaatcaa tgacttccca cacgttgtgg
    12121 aaggcttcga gtgcggagaa atcagctttc actccggctg ggtgtttcat cgggcgggcg
    12181 ccaatcagag ccgcgaaatg cgcaaagtga tgactgtcat ttatatggac aaagagacga
    12241 ctttaatgga accgaaaaat tcgggccaac tcaatgactg gaagacctgg tgtcccggtg
    12301 cggaagtcgg gcagattatc aacacgccgc ttaatccggt tttgtattga ataaatttaa
    12361 agttactgtt tgcgtctttt tcctcgcatg gggataaatg tgttattcat aaagacaata
    12421 gatacaggcg ggagggttct tattctaatg gagcttttgt atttgtttcc tggttgcagc
    12481 tcgaagtcgt tataaagcaa tcgagcactt gatgttccac cgccttccct gttgattata
    12541 ggaattagat tactactcac ctcttgggaa atcagcaagt gtaatgtatc attgttgatc
    12601 tgatagtact ctttatacat gtatttccct tctaaatcag ttctgaattt tagaacagga
    12661 acatcaatgg aagcctcagt ggtatttcta taataaatgg tgattttatc tctcctctgt
    12721 cgtacttccg ttggctgtcc aattgctgaa aaaaaactgc caatgaagat gaaagcaaag
    12781 agagt
//
LOCUS       DAORLF010000072        12780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2620_length_12780_cov_1.980354, whole genome shotgun sequence.
ACCESSION   DAORLF010000072 DAORLF010000000
VERSION     DAORLF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_12780_cov_1.980354"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1385)
                     /locus_tag="PK198_08075"
     CDS             complement(<1..>1385)
                     /locus_tag="PK198_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRF38731.1"
                     /translation="PCLCSASRRPETAASFYQAVQNEAPALAVITEATMEEMAFQSYS
                     DFQIVHSDAAGEASLLLTPDVALCADCRAELHAPSNRRFGYPFITCTLCGPRYSIIQK
                     LPYDRPFTTMQPFPMCPDCAAEYENPLDRRYYSQTNSCADCGISLQWQPGHGIPLTGA
                     PDELIGLAAEAILEGKILAVQGIGGFLLCCDAGNETAIQTLRRRKHRPSKPFALMYPS
                     LESLREDAQLGPEQAEELLGIAAPIVLVDLLPNGHKKLAVKDIAPGLNQVGVMLPYAP
                     LLELLLRRICRPIVATSGNPTQHPIVFDNETALCELPSIADVLLLHNRDIATPQDDSV
                     VRFSPFLHERIVLRRSRGLAPTFILPGRKAAPGTVLAMGAMLKSAFGLRHQGNYYLSQ
                     YLGDLQNFDTERSFQWALRHLLGLLSARPEAVLTDLHPDYYSTRLGEILAGEWQVPVF
                     RYQHHEGHFAA"
     assembly_gap    1386..1395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1614..2114
                     /locus_tag="PK198_08080"
     CDS             1614..2114
                     /locus_tag="PK198_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRF38732.1"
                     /translation="MDYNLAYLFVAALIPLVIGAVWYHPKVFGNAWAKAADMSEAKIN
                     SGNMLVIFGLTYVLSVMAAFIISMLVVHQGHIYSTLMNEPSLMEEGSELNLYVKDFMD
                     KYGSNFRTFKHGAFHGFFTGLFFAMPILAVNAMFERKGFKYIAINAGYWMVSFALMGG
                     LLCALL"
     gene            complement(2129..3160)
                     /locus_tag="PK198_08085"
     CDS             complement(2129..3160)
                     /locus_tag="PK198_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF38733.1"
                     /translation="MLSYWETFLKSYKGYANYFWHEITQPGWNNYFYWLILVSLFFLI
                     LEWLSPWRKEQPRFRKDFWLDAFYMFFNFFLFSLIIYNAASDVVVRFFNESILHLTGF
                     NLKASNPMQYWPAWAVLLTGFVVRDFVQWWVHRLLHRSDRLWEYHKVHHSVEQMGFAA
                     HLRYHWMETLVYRSIEYIPLALLGIGLHDFFVIHIFTLAIGHYNHANISVPGYVTGAV
                     LGLLIGIVAANGLYDVNLFGPLSVLGKTGVMAGFALGGALLLGPLMKRIFNHPEMHIW
                     HHAHDLPEERRYGVNFGISLALWDYLFGTAYIPKDGRDARLGFPGLARFPHNFWGQIT
                     HGFRKKKEA"
     gene            complement(3177..3572)
                     /locus_tag="PK198_08090"
     CDS             complement(3177..3572)
                     /locus_tag="PK198_08090"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRF38734.1"
                     /translation="MAFAYGNLEQQPLKMMKQAKIPRITSINHIDGFILICTFNTGEQ
                     RKLDMLTFFLRNHYSEDSFRSAALDVNKFNTVSLRDGTLCWPGIVRTTRLSNGMEFEE
                     YFDLDPLVLYDISTPVELTADHIAVLTVS"
     gene            complement(3585..3794)
                     /locus_tag="PK198_08095"
     CDS             complement(3585..3794)
                     /locus_tag="PK198_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38735.1"
                     /translation="MKDNKISTLIKDWRTAAVLCLTLGLAPFFPEPHIQGSLDCRRRG
                     RHGTHRLVGFLYARVALGVFALFVE"
     gene            3827..4804
                     /locus_tag="PK198_08100"
     CDS             3827..4804
                     /locus_tag="PK198_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRF38736.1"
                     /translation="MSTNTKNTLPKIVVLDGYNLNPGDLSWAALQALGEVMIYDFTTP
                     EGIVERSEGAAILLVNKAVIHRQIMQLLPDLQCIVVTATGYNNIDVAAARERGITVCN
                     AVGYSSEAVVQHVFALLLELSNAVGRHHDSVQAGDWAAQRSFCYTLQPIHEIAGKTMG
                     IYGFGNIGRRVGEGALAFGMRVLATHKHPERDARPGVRFVPIEQLFEESDVISLHAPL
                     SAANTGIVNAALLSRMKPSALLINTGRGGLVNEEDLLEALQKGNLAGAGLDVLSIEPP
                     AADHPLIGAPNCIVTPHVAWASVQARQRLMDICVQNVRAFLDGAPQNVV"
     gene            complement(4898..6115)
                     /locus_tag="PK198_08105"
     CDS             complement(4898..6115)
                     /locus_tag="PK198_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRF38737.1"
                     /translation="MKRAFQLEDFISRHVTQRDKSLTYYDMVANDPVLRSRIEGRRVL
                     VIGGAGSIGSAFIKAMLPYRPAALVVVDINENGLTELTRDLRSTHELPVPPVFLTYPM
                     SFEAPVFERMFRQEGPFQIVANFAAHKHVRSEKDRFAIEAMLENNVLQAKTLLDLLVQ
                     FPPERFFCVSTDKAANPANVMGAGKKLMENLLLAYADRLPVTTARFANVAFSNGSLPD
                     GFLQRMAKNQPLSAPKDIRRYFVSPREAGEICLLACIAGEPGNIFFPKLDESQLTAFT
                     DIAKALLHELGYKIDYCASEDDAREKAARYLPGADYYPVYFFDSDTSGEKPFEEFVTE
                     GEAVRMDLFQSLGVIAATAPHSLPEMEAQLQRLREVFRSEDTDKGDLVTLMRELIPTF
                     SHIETGKGLDQKM"
     gene            6238..6900
                     /locus_tag="PK198_08110"
     CDS             6238..6900
                     /locus_tag="PK198_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRF38738.1"
                     /translation="MLTENALRFLSDLSLNNDRDWFHANKARYEKDVKKPFEAFVAAL
                     IERAQALDPAILITPRQAIFRIHRDTRFSANKEPYKTHVSAIISPFGTRHKEYPGFYV
                     QVSADKLWLGGGAYFLEKESLLKIRETIAENGEELTDIVQHPDFVQHYGEVLGEKNKR
                     LTPEFQTIATQMPLIANKQFYFMAELEPEAALGAQALDRAMTYYLAGKPLCDFLAQAM
                     GD"
     assembly_gap    7098..7143
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7144..>7447
                     /locus_tag="PK198_08115"
     CDS             <7144..>7447
                     /locus_tag="PK198_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38739.1"
                     /translation="DSYKTALARIFFARGEYSKCNVLLEALLNSNYDDMDARLLHAKA
                     LLREQQPEAAREAYRKVLELDPGFTDEELDQALRLPAASDDLLDEILGDDNLIFKPE"
     assembly_gap    7448..7573
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7574..>7850
                     /locus_tag="PK198_08120"
     CDS             <7574..>7850
                     /locus_tag="PK198_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38740.1"
                     /translation="GGILLYGPPGCGKTFIARATAGQINNLFLSVGLEDILDMWVGSS
                     EKNLHHIFETARDNKPCVLFIDEIDALGASRNDMRHSAGRQLINQFLM"
     assembly_gap    7851..7951
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7952..8314
                     /locus_tag="PK198_08125"
     CDS             <7952..8314
                     /locus_tag="PK198_08125"
                     /inference="COORDINATES: protein motif:HMM:NF037139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HRF38741.1"
                     /translation="RFDRIIFVPPPDESARQSIFRLKLKDKPVENIDFQQLAKKTEHY
                     SGADIEAVIDLAVEEKLTDAFQTGQIQALRTKDLLTAITKHKPSTSEWLATAKNYALF
                     ANNSGLYDDVLSYLKIKK"
     gene            8311..9567
                     /locus_tag="PK198_08130"
     CDS             8311..9567
                     /locus_tag="PK198_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38742.1"
                     /translation="MMNRDVLQKVEILIQQEKYREARRILEDLLPQSPDDGFIYMALA
                     EVYQNLKEYDKALELINTAIGLEPESGDAYQRKANILLSMDRFKEADDVLDRAIELSP
                     WDADNYGLRAFIMAANLQFEGALYWADKTLEADPKNLLGLNIRSTSLMKLNRQEESFQ
                     TIENALEEDPDNPYTHANHGWNLLERGDHQKALIHFREALRLDPDMEYARDGMADALQ
                     SKYWPYRMFMKYAFWMGNLTEKYKWGVIIGLYLALNALNNLAERNETLRPYLYPVVVA
                     ITIWAFSTWITRPLSNLTLRFHPFGKYLLNDRGKMATNFVGLSLLLCLVGGLGYLISG
                     HFGWAALGGFGLTMMLPLGSMLTRQSNYILPVYTGVLAILGLWAVVIAFSVNEMWNTV
                     ALIYIGGVFVYQWVANFVIMRSNRYL"
     gene            9698..11137
                     /locus_tag="PK198_08135"
     CDS             9698..11137
                     /locus_tag="PK198_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF38743.1"
                     /translation="MMKSPIHLYGTLLLAFFISTAAVAQSTLWSLEKCVNYALQNSLR
                     IKQAQATLRDGELSVKQGQMSRLPSVNASSGLGAQFGRTIDPFTNTFATSSITFNSFG
                     LNAGILLYNGGSVNAGINRSKLELEARQYDMENTSNTLALEVARAYLTILLAQEQLDN
                     AQRQLTLSRQQLDQTSKLINAGALPPNDRLDVEAQIARNEQVVVEVRNAVTGAYLTLR
                     QLMELPAEETLVIERPQINVPEVVDEATFAFPTVYSTALGNQPQIKAADIRLRAASHD
                     ITIARSGMLPTITFGGNLNTAFSSVAQRIAGINTVRQSQIVFINGQTVTIETDVPSAE
                     FEKIPYFSQLSNNFGQSVGLNISIPIYNNHRNSIAVERAQLGVLTQELQNRQQRQFLQ
                     TAVQQAINDTRAAREAWLAGRRAVEAAQAAFDNAQKRFDLGAINALQYSTARITLDSA
                     QVDLIRAKYQYLFNLKNVEFYMGKPLQLN"
     assembly_gap    11233..11268
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11365..11664
                     /locus_tag="PK198_08140"
     CDS             11365..11664
                     /locus_tag="PK198_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRF38744.1"
                     /translation="MAKRKNNNWILFGLLGVVALLVVVAVVKQKSKPKGEQVMVEKAE
                     TRTIKEKVAASGKIFPVTEIKISSDVSGEVVELYVEEGDSVVAGQLLTQMPISRR"
     gene            11682..12770
                     /locus_tag="PK198_08145"
     CDS             11682..12770
                     /locus_tag="PK198_08145"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF38745.1"
                     /translation="MRSAQAQAANARSQIETLRAQKEQIEAQLTNAREIHNRNEKLKK
                     EGVISDADFDNSLSNLRALEANLRSSDASIKASQESVRAAEFQIQSAEAGLRELQTSL
                     RRTTQYAPKGGIISKLNIEKGERVVGTIQMAGTEMMRIADLSTMEVRVDVSENDIPRV
                     SIGDLVDIEVDAYIGRKFKGRVTQIANSANSAGGLGAALTTDQVTNFEVRILIEPASY
                     ADLVTPKKPYPFRPGMSASVDINTQTLKDVLSVPIQAVTTREKEGLKKKTTTNEDGEE
                     VEAEKTLDDLIEVVFVAKGDTAHLVEVKTGIQDDSYIYIISGLKAGDEVISGPYTAVS
                     RKLKQGMVIRRATEDELNPAKKKKKEED"
BASE COUNT         3135 a         3355 c         3276 g         2695 t
ORIGIN      
        1 ccgccgcgaa atgcccctcg tgatgctgat acctgaacac cggaacctgc cattccccgg
       61 ccaatatctc gcccagccgg gtggaataat aatccgggtg caggtcggtc agcaccgcct
      121 ccggccgggc cgaaagcagg ccgagcagat gccgcagcgc ccattggaaa ctgcgttctg
      181 tatcgaaatt ctgaagatcg ccgaggtact gactgaggta ataattgccc tgatggcgca
      241 atccgaaagc gcttttcagc atggcgccca tagccagcac agtacccgga gcagccttcc
      301 gtcccggcag gatgaaggtt ggggccagtc cacgagagcg ccgcaaaacg atgcgctcat
      361 gtaaaaacgg actgaaccga actacggaat cgtcttgcgg agtggcaata tcccggttgt
      421 gcagcagcag cacgtcggcg atggagggca gttcgcacag ggcggtttcg ttgtcgaata
      481 cgatgggatg ctgcgtggga ttgccgctcg tagccacgat ggggcgacat attcggcgca
      541 acagcaattc gagcagcgga gcgtagggca acatgacacc cacctgattc aaacccggcg
      601 caatgtcttt gacagccagc tttttatggc cgtttggcaa caaatccacc agcacgatag
      661 gcgccgcaat gccgagcaat tcctctgcct gttccgggcc gagctgcgca tcttcccgga
      721 gcgattccag cgaggggtac atcagcgcga agggcttcga ggggcgatgt ttgcggcggc
      781 gcagggtttg aatggccgtt tcgttgccgg catcgcagca caacaaaaag ccgcctatgc
      841 cttggacggc caaaatttta ccttccaaaa tcgcttctgc cgcgagaccg atcagttcat
      901 ccggcgcgcc tgtcagaggt attccgtgtc ccggctgcca ttgcagggaa atgccgcagt
      961 cggcacagga attggtttga gaatagtagc gccggtccaa tggattttcg tactccgccg
     1021 cgcaatccgg gcacatcggg aagggctgca tggtggtgaa ggggcggtcg taaggcagtt
     1081 tttggatgat ggaatagcgc ggcccgcaga gggtgcaggt gatgaacggg tagccgaaac
     1141 gccggttgga aggtgcgtgc aactccgcgc ggcaatcggc gcaaagcgct acatcgggcg
     1201 tgagcaatag cgaggcttct ccggctgcat cgctgtgaac gatctggaaa tcgctgtaag
     1261 actgaaaagc catctcctcc atcgttgcct ccgtgatgac ggcgagcgca ggcgcctcat
     1321 tttgaacagc ttgataaaaa gaagccgccg tctccggcct gcgcgaagcc gagcaaaggc
     1381 aaggcnnnnn nnnnnattga actcaatgtg tacgccgtcc aatgtgttgt tcacccagcc
     1441 gcacaatcca aaccggttcg ccaattgata cacgaacggc cgaaagccga cgccttgtac
     1501 ctgaccttgc aggtggatgt ggtatgtgcg gtgtggaggc aactttaaaa cttttttttt
     1561 aattttttta taaaaacaaa aaaaccgatt acatttgcac aaaactacgg attatggatt
     1621 acaatctcgc ctacttattt gtggctgcct tgattcccct cgtgatcggc gccgtatggt
     1681 atcatcccaa agtattcgga aatgcctggg ccaaagctgc cgacatgtcc gaagcaaaaa
     1741 tcaacagcgg taacatgctg gtcattttcg gcctcactta tgtactgagc gtgatggcgg
     1801 cttttatcat cagcatgttg gtcgttcatc agggccatat ttattcaaca ttgatgaatg
     1861 aacccagcct gatggaagaa ggaagcgaac tgaatttgta tgtcaaagac ttcatggaca
     1921 agtacggcag caatttccgc accttcaagc acggcgcatt ccacggcttt ttcacgggtt
     1981 tgtttttcgc catgccgata ttagccgtca acgcaatgtt tgaaagaaaa ggcttcaaat
     2041 acattgccat caacgctggc tactggatgg tgtcttttgc gctgatgggc gggctgctgt
     2101 gcgctttact gtaagcgttt tcccaacctt acgcctcttt tttcttgcgg aaaccatgcg
     2161 ttatctgccc ccaaaagttg tgcggaaagc gggctaaacc ggggaagcca agacgcgcgt
     2221 cgcggccgtc tttgggaatg tatgcggtgc cgaaaaggta gtcccacagc gccagactga
     2281 tgccgaaatt gactccatag cgacgctctt ccggcaggtc atgggcgtgg tgccaaatgt
     2341 gcatttccgg gtggttgaag atgcgtttca tcagcggtcc caacagcagc gcgcctccca
     2401 gtgcgaaacc ggccatgact ccggttttgc ccaatacact cagggggcca aagaggttga
     2461 catcatacaa gccgttcgcc gctacaatgc cgatcaacaa accgagcaca gccccggtga
     2521 catagccggg cacgctgatg ttggcgtggt tgtaatgacc aatggccaag gtaaagatgt
     2581 gaatgacaaa gaaatcgtgc aggccgatgc cgagcagcgc cagcggaatg tactcaatgc
     2641 tgcgatatac cagcgtttcc atccagtggt agcggaggtg cgcagcgaag cccatttgct
     2701 ctaccgagtg gtgtactttg tggtattccc atagccggtc ggaacggtgc agcaggcggt
     2761 gaacccacca ttgtacaaaa tcgcgcacca cgaagccggt gagcagcacc gcccaggcag
     2821 gccagtattg catgggattg gaggctttga ggttgaagcc cgtgaggtgc aggatgcttt
     2881 cgttgaaaaa gcgcaccacg acatcggaag ccgcattgta aatgatgagt gaaaagagga
     2941 aaaaattgaa aaacatgtaa aaggcatcta accagaagtc tttgcgaaag cgcggctgct
     3001 ctttgcgcca ggggctgagc cattcaagga tgaggaaaaa caaggacact aaaatgagcc
     3061 agtagaagta gttgttccag cccggctgcg tgatttcgtg ccagaagtaa ttggcgtagc
     3121 ctttgtagct tttgaggaag gtttcccagt aagagagcat ggaaaaagtt tgaaattcaa
     3181 gaaactgtaa ggactgcgat atggtctgcc gtcaattcta caggagtgct gatgtcgtat
     3241 agaaccagag gatccagatc aaaatattct tcaaactcca tgccatttga aagccgtgta
     3301 gtacgaacga tgcctggcca gcagagcgtg ccgtcacgaa gagataccgt attaaacttg
     3361 tttacatcca acgctgccga cctaaatgaa tcctccgaat aatggttcct taaaaaaaag
     3421 gtcagcatat cgagtttcct ttgctctcct gtgttgaagg tgcaaatcag aatgaaacca
     3481 tcaatgtggt tgatggaagt gatacgtggt attttagctt gcttcatcat tttgagtggt
     3541 tgttgttcca gatttccata agcgaaagcc atcagaataa tttcttattc aacaaataaa
     3601 gcaaatacac ccagggcaac ccgtgcataa agaaatccaa ccagtctatg agtcccatgc
     3661 ctaccgcgcc gccggcaatc cagcgaacct tgaatgtgcg gttcggggaa aaaaggcgcc
     3721 aggcccaacg tgagacacaa tacggctgcc gtgcgccagt cttttatcag agtagatatt
     3781 ttattgtctt tcattgctgt atttttgagg cttcaaaagt agtgaaatga gcacgaatac
     3841 caagaatacc ctgccgaaaa ttgtggtttt ggacggctac aacctcaatc ccggcgacct
     3901 gagttgggcc gcgttgcaag cccttggcga ggtgatgatt tatgatttta ccaccccgga
     3961 ggggatcgtt gaacgttcgg aaggagcagc gattttgttg gtcaacaaag ccgtcatcca
     4021 tcggcaaatt atgcaattat tgcctgattt gcagtgcatt gtagtcactg ccaccggata
     4081 caacaacata gacgtcgccg ctgcccgcga acggggcatt accgtgtgca atgccgtggg
     4141 gtacagttcg gaggcagtgg tgcagcacgt ttttgcgttg ctgttggaac tgagcaacgc
     4201 cgtaggtcgc caccatgatt cggtgcaggc aggcgactgg gccgcgcaac ggagtttttg
     4261 ctacaccttg cagcccatcc acgaaattgc cggcaaaacg atgggcattt acggcttcgg
     4321 caacatcggc cgccgggtag gagaaggggc gctggctttc ggaatgcggg tattggccac
     4381 acacaaacat cccgaacgcg acgcccggcc cggtgtgcgc tttgtaccca tcgaacagct
     4441 ttttgaagaa agcgacgtca tttcgctgca cgcgccgctc agcgcggcca atacaggcat
     4501 cgtcaacgct gccttgctgt cgcgcatgaa accctcggcc ctgctcatca acacggggcg
     4561 cggcggtttg gtaaacgagg aagacctgtt ggaagcattg caaaaaggca atttggcggg
     4621 cgccggtttg gacgtactat ccatagagcc gccggccgca gatcacccgc tcatcggggc
     4681 gcccaactgc attgtgacgc cgcatgtagc ttgggcttcg gtgcaggccc ggcagcgatt
     4741 gatggatatt tgtgtgcaga atgtgcgggc ttttctggat ggagcgccgc agaatgtggt
     4801 gtaaaagctt acatcttctg atccaatccg ctcgcgcaag gttctacctt gtgcggatta
     4861 tcacgcgggt tctacccgcc tgccataccc gcccttttta catcttctga tccaaccctt
     4921 ttccagtttc gatgtggctg aaagtaggaa tcaactcccg catcagcgtc accaaatcac
     4981 ctttgtcggt atcctcagag cggaagacct cccgcaggcg ttgcaattgc gcctccattt
     5041 ccggcagcga atgtggcgcc gtggcggcaa tgacccccag cgattgaaac aaatccatgc
     5101 ggactgcctc gccttccgtc acaaactcct cgaacggctt ttcgcccgat gtatcggaat
     5161 cgaaaaaata cacggggtaa taatcggcgc cgggcaggta gcgagccgcc ttttcgcggg
     5221 catcgtcttc agaggcgcaa tagtcaattt tgtagcccaa ttcgtgcaag agtgctttcg
     5281 cgatgtcggt aaaagcggtg agttgacttt cgtccaattt cgggaagaag atattgccgg
     5341 gttcgccggc tatgcaggcc agcaggcaga tttcaccggc ttcgcggggc gatacgaaat
     5401 agcggcggat gtctttgggc gccgacagcg gctggttttt ggccatccgt tgcagaaatc
     5461 cgtcgggcag gctgccgttg gaaaacgcca cattggcaaa gcgggcagtc gtcaccggca
     5521 gtcggtcggc gtaagccagc aacaggtttt ccatcagttt tttacccgcg cccatcacat
     5581 tggcagggtt ggcggctttg tcggtactga cgcaaaagaa gcgctccggc ggaaattgaa
     5641 ccagtaaatc gagcagggtt ttggcctgca acacattgtt ttccagcata gcttctatgg
     5701 cgaagcgatc tttttcggaa cggacgtgtt tgtgcgccgc gaaattggcc acgatctgaa
     5761 acggcccttc ctgccggaac atgcgctcaa ataccggcgc ctcaaacgac atggggtaag
     5821 tgagaaaaac aggcggcacg ggcagctcgt gggtgctgcg caggtcgcgg gtcagttcgg
     5881 taaggccgtt ttcgttgatg tccacgacca ccaaagcggc gggtcggtat ggcaacatgg
     5941 ctttgatgaa cgcgctgccg atgctgcctg cgcctccgat gaccaatacg cggcggcctt
     6001 caatgcggct ccgcaacacg gggtcgttgg ctaccatgtc gtagtaagtc aggcttttat
     6061 ctcgctgagt gacgtgccgg gagatgaaat cttccaactg aaatgctcgc ttcatgttcg
     6121 ctttggcttg gtttgtcggg cgaaggtatg gaacgcggcc ggaataaatg ggtgtaaaat
     6181 gttggtatct gtcttatctt gcaacgcctt ttctacacaa taaaaaaacc atgcgtcatg
     6241 ctgaccgaaa atgccctccg atttctttcc gacctgtcgc tcaacaatga ccgcgactgg
     6301 tttcatgcca acaaggcccg ctatgaaaaa gacgtcaaaa agccgtttga agcctttgtg
     6361 gcggcgctca tcgaacgggc gcaggcactc gacccggcca tattgattac gcctcggcag
     6421 gccattttcc gcatccatcg ggatacgcgc ttttcggcca acaaagagcc gtataaaacg
     6481 catgtatcgg ccatcatttc gcccttcggt acgcggcaca aggagtatcc ggggttttat
     6541 gtgcaggtat cggcagataa gttgtggctg ggtggcggcg cttatttttt agaaaaagag
     6601 agtttgctaa aaatcaggga aaccattgct gaaaacggcg aagaactgac cgacatcgtt
     6661 caacaccctg attttgtgca acactacggc gaagtgctgg gcgaaaaaaa caaacgcctc
     6721 acacctgaat ttcagacgat tgcaacccaa atgccgctca ttgccaacaa gcaattttac
     6781 ttcatggccg aattggaacc cgaagcagca ttgggcgcac aggcattgga cagagctatg
     6841 acttactatc tggccggcaa gcctttgtgc gattttctgg cgcaagcgat gggagactga
     6901 aattacattt cttacctttg tcgcctgtct aaggggcaat aaaaaataaa tttgccccac
     6961 tacaaaactg taaaaattcc cacatggacc accaagcaat cgaaaacctc agagtagcgc
     7021 tgcgcgagtc gccgcacaat attcccttga aacaaatgct ggccgaaacc cttctgcggg
     7081 caggcctctg ggacgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnggacagt tacaaaaccg cattggcccg catatttttt gcccgcggcg aatactccaa
     7201 atgcaacgtg ctgctcgaag cactgttgaa cagcaactac gacgatatgg atgcccggct
     7261 gctgcacgcc aaagccctcc tgcgcgaaca acaaccggag gccgcccgcg aagcataccg
     7321 caaagtgctg gaactcgacc ccggatttac agatgaagaa ttagatcagg cattgaggct
     7381 cccggcagct tccgacgacc tgctggacga aatactggga gacgacaatc ttatttttaa
     7441 acccgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnggcggta tcctgctgta cgggcctccg ggctgcggca aaaccttcat
     7621 tgccagggct acggccggcc agatcaacaa cctgtttctc agcgtggggc tggaagatat
     7681 tttagatatg tgggtcggga gcagcgaaaa aaacctgcac catatttttg aaaccgcccg
     7741 cgacaacaaa ccctgcgtgc tttttattga tgaaatagac gctctgggcg ccagccgaaa
     7801 cgacatgcga cactctgccg gacggcaact catcaatcag tttttgatgg nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggtttgat cgcatcattt ttgttcctcc
     7981 accggatgag tcggcccggc agtcaatatt ccgtttaaaa ctcaaagaca aacccgtcga
     8041 aaatattgat tttcaacaac tcgcaaaaaa aaccgagcat tattcaggcg ccgacatcga
     8101 agccgtcatt gacttggccg tagaagaaaa actgacggat gcttttcaga caggacaaat
     8161 acaagccctt cgtaccaaag acctcctgac ggccattacc aaacacaaac ccagtacttc
     8221 cgagtggttg gccaccgcca aaaattacgc cttgtttgcc aataactccg gcctttacga
     8281 cgacgtactg agctacctca aaatcaaaaa atgatgaatc gagacgtatt gcaaaaagta
     8341 gagatactga tacagcagga aaagtacaga gaagcccgcc gcatcctgga agacttgctc
     8401 ccacagtcgc ccgacgacgg gttcatttat atggcattag ccgaagtgta tcaaaatctg
     8461 aaggagtacg acaaggcact ggaattgatc aatacagcca ttggcttaga gccggaatcg
     8521 ggagacgcgt accagcggaa ggctaatatt ctactttcta tggatcgctt caaggaggcc
     8581 gacgatgtat tggaccgggc cattgaactg tcgccctggg atgccgacaa ttatgggttg
     8641 cgggcattca taatggcagc aaatttacag tttgaaggcg cgctctactg ggccgacaaa
     8701 acattggaag ccgatccgaa aaacttgctg gggcttaaca tcagaagcac ctctctgatg
     8761 aaacttaaca ggcaggaaga gtcttttcaa accatagaaa acgcgcttga agaagacccc
     8821 gacaaccctt acacccatgc caatcatggt tggaatttat tggaaagagg cgatcatcaa
     8881 aaagcgctta tccacttcag ggaggccctc cgccttgatc ccgatatgga atacgcccgt
     8941 gacggcatgg ccgacgcact gcaaagcaag tactggccct acaggatgtt tatgaaatat
     9001 gccttctgga tgggcaacct caccgagaag tacaaatggg gggtgatcat tggcttgtac
     9061 ctggcattga acgccctcaa caatctggcc gaacgcaatg agacactgcg tccgtacctg
     9121 tatcccgtgg tagtggccat aaccatttgg gctttttcta cctggatcac gcgcccattg
     9181 agcaacctga cgctgagatt ccaccccttc ggaaaatacc tgctgaacga ccggggcaaa
     9241 atggctacca attttgtggg tctgagcctg ctgctttgtt tggtcggggg gctgggatac
     9301 ctcatttcag gccatttcgg ctgggcggct ctggggggat tcggacttac catgatgctt
     9361 cccttaggtt cgatgctgac ccggcaatcc aattatatat tgcctgttta tacaggcgtt
     9421 ttggcaatac tgggactgtg ggcggttgtc attgcttttt ccgtcaatga aatgtggaac
     9481 accgtagcgc ttatttacat cggcggggta tttgtttacc aatgggtagc caattttgtc
     9541 atcatgcgct ctaatcggta tttgtagaaa tctttaccgg ccacgcctca caaaacacgc
     9601 cctttagcgg gctttcatcc aaataccctg ctcattgata gaataatttg gcctgtgccg
     9661 ccatgcaggc gtactattgt gggacaaaaa tcattgcatg atgaagtctc cgattcacct
     9721 gtacggaacc ctgttgcttg cttttttcat cagcacagcg gccgtagcac aatccaccct
     9781 ctggtctttg gaaaaatgcg tcaactacgc gttgcagaac tcccttcgca tcaagcaggc
     9841 gcaagccacc ctgcgcgacg gcgagctttc tgtaaaacaa ggccaaatga gccgcctgcc
     9901 ttccgtgaac gccagcagcg gtctgggggc gcagtttggt cgcaccattg accccttcac
     9961 caacaccttt gccaccagct ccattacctt caacagcttt ggcctcaacg caggcatatt
    10021 gctgtacaac ggcggcagcg tcaatgccgg catcaacagg agcaagttgg aactggaagc
    10081 gcgccaatac gacatggaaa acacctccaa taccctcgca ctggaggtgg cccgcgctta
    10141 tcttaccatt ctgcttgcgc aggaacaatt ggacaatgcc cagcgccaac ttaccctctc
    10201 gcggcagcaa ttggatcaaa cttccaagct catcaatgcc ggcgccctgc cgcccaacga
    10261 ccgcttagat gtggaggcgc aaatagcccg caatgagcag gtggtcgtcg aagtgcgcaa
    10321 tgccgttacc ggcgcttatc tcaccctgcg ccaattgatg gaattgcccg ccgaagaaac
    10381 attggtcatc gagcggcctc aaatcaatgt gcccgaagta gtggacgaag ctactttcgc
    10441 ctttcctacg gtgtattcca ccgcattggg caaccaacct caaatcaaag ccgccgacat
    10501 ccgcctgcgc gctgcttctc acgacatcac catcgctcgc tccggtatgt tgcctaccat
    10561 taccttcggc ggcaacctca ataccgcatt ttcctctgtg gcacaacgaa ttgcagggat
    10621 caatacggtg cggcaatcgc agattgtctt tatcaatggc caaacggtta ccatcgaaac
    10681 ggacgtaccc tcggcggaat ttgaaaaaat tccgtacttt tctcagttga gcaacaactt
    10741 cgggcagtcg gtcggcctca atatttccat tccgatctat aataaccacc gcaacagcat
    10801 agccgtagag cgcgctcagt tgggcgtact cacccaggaa ttgcaaaatc gccaacagcg
    10861 gcaattcctc caaaccgccg tacagcaggc catcaacgat accagagccg cccgtgaagc
    10921 ctggttggcg gggcgccgtg cagtagaagc ggctcaggct gctttcgaca atgcgcagaa
    10981 acgcttcgat ctgggcgcca tcaacgcgtt gcaatacagc accgcccgca ttaccctcga
    11041 ttcggcgcag gtggacctca tccgggccaa gtatcaatac ctcttcaacc tgaaaaacgt
    11101 agagttttac atgggtaaac ctttgcagtt gaattgataa ccggaaggag cggtcagctt
    11161 tagtgcggca aatggcctga taaaagctga gcgctctgcc ggcgcgtgag ggggctgaag
    11221 gaagcgacct tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ggggccacag
    11281 gcttctacca gcgccccggc acgccaccca aaactgtacg catcattctt tcattccgag
    11341 caacttaaat aaaagacgaa agtcatggca aaacgcaaaa ataacaactg gattctcttc
    11401 ggccttctgg gcgtagtggc gctgctcgta gtagtggccg tagtgaaaca aaagtctaag
    11461 cccaagggcg agcaagtgat ggtggaaaag gcggaaacgc gcaccatcaa agaaaaagta
    11521 gccgccagcg gcaagatttt cccggtcacg gaaatcaaaa tcagctccga cgtatcaggc
    11581 gaggtggtcg aattatatgt ggaagaaggc gactcggtag tggccggtca actgctgacc
    11641 cagatgccta tcagtcgcag gtagagcgcg gccaggcagg cgtgcgcagc gcacaggcac
    11701 aagccgccaa cgcccgcagc caaattgaga cgctgcgcgc acaaaaagag cagattgagg
    11761 cgcaactgac caacgcccgc gaaattcaca atcgcaacga gaagctgaaa aaagaaggcg
    11821 ttatctccga tgccgatttc gacaattcgc tgtccaacct ccgtgccctc gaagccaatc
    11881 ttcgctcttc cgacgccagt atcaaggcct cgcaggaaag cgtgcgcgcc gccgagtttc
    11941 agattcagag cgccgaagca ggattgcgcg aactgcaaac cagcctccgc cgcaccacgc
    12001 aatacgcacc caaaggcggc atcatctcca agctcaacat cgaaaaaggc gagcgcgtag
    12061 taggtaccat ccagatggcc ggcaccgaaa tgatgcgcat tgccgacctc agcaccatgg
    12121 aagtgcgcgt ggacgtgagt gaaaacgata tcccgcgcgt ctccatcggc gatttggtgg
    12181 acatcgaagt ggacgcctac attggccgca agttcaaggg ccgcgtcaca caaattgcca
    12241 actctgccaa ctccgccggc ggcttaggcg ccgccctcac taccgaccag gtgaccaact
    12301 ttgaagtgcg catcctcatc gaaccggcct cctatgccga cctcgtgaca cccaaaaagc
    12361 cctatccttt ccggccaggt atgtccgcct ccgttgacat caacacccag acgctcaagg
    12421 atgtactcag cgtgcctatc caggccgtca ccacccgcga aaaagaaggg ctgaagaaaa
    12481 aaaccactac caacgaagat ggagaagaag tagaagcgga aaaaactctc gacgacctca
    12541 tcgaggtagt cttcgttgcc aaaggcgata ctgcccatct cgtggaagta aaaaccggca
    12601 tccaggacga ctcttacatc tacatcattt ccggtctcaa agccggcgac gaagtcatca
    12661 gcggccccta caccgccgta tcccgcaaac tcaagcaggg catggttatc cgtcgcgcca
    12721 cagaagacga actcaatccg gccaagaaga aaaagaaaga agaggattaa caattgtttt
//
LOCUS       DAORLF010000073        12750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2623_length_12750_cov_2.247499, whole genome shotgun sequence.
ACCESSION   DAORLF010000073 DAORLF010000000
VERSION     DAORLF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_12750_cov_2.247499"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    243..281
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <282..>979
                     /locus_tag="PK198_08150"
     CDS             <282..>979
                     /locus_tag="PK198_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38746.1"
                     /translation="YLRQILTDDRLLADALRAHGLRVARKDWADPDFDWTQTRAAVFR
                     TTWDYFHRFGEFSAWLERVTTQTQLLNAPETIRWNMDKHYLLDLEAQGIRIPPTRMVH
                     KGEVTTLEALHRETGWQGQETILKPTISGGARHTYRLNPDSWAAHEEVFQQLIAQEDM
                     MLQPLQHRVLSEGEMSLMVMGGRFTHGVLKIAKPGDFRVQDDFGGTAHPHTPAPEAIA
                     LAERAAAACSPLPL"
     assembly_gap    980..1068
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1186..>1429)
                     /locus_tag="PK198_08155"
     CDS             complement(1186..>1429)
                     /locus_tag="PK198_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38747.1"
                     /translation="DYQLPAGVIANQLVVELREETDQDAWLRNYAKQDMKLLKKVWPT
                     NNKIIVSYSTKVINPPAMLELVRQDTEVLSAEYNRK"
     assembly_gap    1430..1501
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1502..1902)
                     /locus_tag="PK198_08160"
     CDS             complement(<1502..1902)
                     /locus_tag="PK198_08160"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HRF38748.1"
                     /translation="MKNPVLLWPVLVLAAAITSCNPPLSLVATDKLAPVIEMSKGACF
                     GRCPVFTLTIYSNGVAAYKGERHTDKMGLYLKRLPKSELESLLLAFKKADLWQYPDVY
                     RSQFPDLPAVNITYHEAERSKSILGKETRPEP"
     gene            complement(1937..3187)
                     /locus_tag="PK198_08165"
     CDS             complement(1937..3187)
                     /locus_tag="PK198_08165"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF38749.1"
                     /translation="MALRLILIGCLVGLLSSSELWAQRCVSQERLAAYLQQHPDAEAA
                     RADLENFIQLQQAVQTRSTIDIPVVFHVVWNTAEENISDEQILSQLEVLNRDFRRTNN
                     SAGLIPSLFQSLAADMELNFCLARRSPDGASTNGILRRQTSFPFMGDRIANGRKSVCY
                     TAEGGSDAWDTGRYVNVWVARRQFFPAEASFPGMGAAAEDGIIISPPFVGTTGSAASN
                     QPYHLGRTLTHELGHYFNLYHLWGPGQTGSCTQSDEVADTPLQSRSYLGECPTHPQIS
                     CGSADMFMNFMNYTDDACMAMFSNGQKNRVWAAIAAARPGLMLAAQACSPVSVVQAPQ
                     QEALFELLQNPTGESLLLRTMAPAGQRFHWQLVSMWGAPLRQGTLLIHAGLQSVDVSG
                     LSAGMYVLRIGGEGVLWSGKVVIE"
     gene            complement(3191..4159)
                     /locus_tag="PK198_08170"
     CDS             complement(3191..4159)
                     /locus_tag="PK198_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF38750.1"
                     /translation="MRLLPLIAVLALLSGCISKASYEELTESRNYYKQESKAADSLRL
                     ANNTLYERNRNLEAELNKAEMAINDASITNAALHRNYNELLTDYNRVVTSNRDVLSSS
                     AYEKQSLTERLAAQQAELDRKQREAAAMEYAIQSKEERLGAVQTYDQMTAKGTTSASQ
                     QELNNLLDAQQRQMNQLQQNLGRILAPYGASAVQINRTDNNRLNVSLQHSLLFTDGGI
                     QPGTQGQQVIQQIASALASFPDVEVLVVGRGDSYGASDVNWEYGIQRASSVGRFLAAG
                     GVQPGKILAAGQGPQFSTAFGIKAVVNQTDIILSPNMTRVMQLLGQ"
     gene            4279..4863
                     /locus_tag="PK198_08175"
     CDS             4279..4863
                     /locus_tag="PK198_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRF38751.1"
                     /translation="MNKMIIVWMTALLLSACAGEGGNSKAVRQQAKEAIEAGTATPAA
                     PAEEASPQITGNIVMTLPKGTLAAGATQCFEVKVSGFKDILATQYTLNWDSKVMNFKE
                     IKEFKLPFMTKENFGIALTPNGKLSSVWIENSLKGATIPDGGAIYQLCFEATGKAGQS
                     SELWVSGTPTAIEVVTKGDQVWGINSVKGKITVE"
     gene            complement(4928..7414)
                     /locus_tag="PK198_08180"
     CDS             complement(4928..7414)
                     /locus_tag="PK198_08180"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF38752.1"
                     /translation="MKRNAFLLIAMLLAGVAAFAQSSAPQKRRMTALRTEERITIDGV
                     LNEQVWANAAIAADFVQTSPVPGAASAFTTQIKVIYDNSGIYIGAQMLDPRPDSILQE
                     LTQRDRLGNTDVFGVFIDAYQDGLNGLGFLVTPAGVQVDLKIAPVSGGDNDGNQIVTQ
                     GEDINWDAVWYNKSIINDKGWTAEIHIPFAALRFPKTPVQSWHINFGRSVKRLNENSF
                     WNYIDPQVNGFLIQSGIIEGFENIKAPLRLQATPFVAVYGQHYHDKNSDPKNSLGYSF
                     NGGMDVKYGISDAFTLDMTLIPDFGEAQSDNQILNLSPFEVQFNENRQFFTEGTELFN
                     KGGLFYSRRIGGTPIKYWDVEDQLQEGETIVSNPQQAKLINASKISGRTAKGLGLGLF
                     NATSARSHAVIRNAEGLEREVETDPLTNYTVMVADQNLKNNSFVSLVNTTAWRSGADY
                     DANVSAAVFDFRNKGNSWGLRGRGVLSQLYHPGQVDLGHSWNIGIGKISGKWQGGLSY
                     VEESDTYNPNDLGFLYNNNERSVGAEINYQQYQPFGKFNSAGGGAYVNYNRLYKPNVF
                     TDFGINFWAWAQTKTFWNFNVFTYHEPVRTFDYFEARTPGRFYHFPTNNNVGFWFGTD
                     NRKQIRISMNSNFRTFADEGRYRWNTSISPRYRVNDKLTLSLSTYYGHWYNDVGFADK
                     IENPATGVTDVIFGRRLIQTVENVFNTSYTFNPNTALTLRARHYWTNVEYSRYHLLTE
                     DGNLGETSHNPERDANFNAFNVDMIFRWRFAPGSDLFVIWKNSVLHADALAGEDYFSN
                     LDNLFDAPQNNSLSIKLIYFLDYQRLVRRG"
     gene            7785..>8645
                     /locus_tag="PK198_08185"
     CDS             7785..>8645
                     /locus_tag="PK198_08185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF38753.1"
                     /translation="MKNLIPHFIQDQSLAGKTYGRMQAYTMFVDMSGFTALTETLMAQ
                     GARGAEQLSQALNDIFSPLVRLVYSRGGFIPYFAGDAFTAIFPANSPRVTATGFIQTA
                     IIARDMFAGKELHLGAFSISIKIGLSFGNVEWGIVGGNRQAYYFRGLPVDHSAQGQSM
                     AGKLEIVVDAALCKMLDGQGFSLSELSGGFKKLNDTPPVRLHSGKAAELPELNEAVGL
                     RFLPREVLDFNQSGEFRTVIAVFLSFDGVDNHELLDRFAGIALEQADNFSGYFKEIDF
                     GDKGGVMVVFF"
     assembly_gap    8646..8807
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8808..11812
                     /locus_tag="PK198_08190"
     CDS             <8808..11812
                     /locus_tag="PK198_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38754.1"
                     /translation="CQYAAVGNRVNLAARLMSHADWGEALVDNELRKNRQYRFYHKGD
                     IKYKGIKGNVPTYLLLGRNLEYQHSFSGPLVGRTREMEQLLRFSEPLTKGVSPGTVVV
                     YGEAGSGKSRLCFELRNSLQKSDLAEWYTCAADQILQKPLNAFLFFLRNYFDQSLEAA
                     PRENRRRFEAGFDALIAELEQKKAPGYEFMQRELWRIRSVLAGLLGIHYPDSLWEQLD
                     AKGRYENTTQAILHLLNVEAALQPFVLEIEDAHWLDDQSAELLHEIVRRLHKWPILIL
                     VTSRYRDDGKEPEILPEAAQAEQLVIRLSPLEAESVRRFAELHLQGKISPGFYEQLLR
                     HTNSNPFYVEQVLEYCVERNLLRQENGVWHVTAEDIDLSGSIQSILTARIDRLSTLAR
                     ETVKAAAVIGREFEVPVLNEVMIHQEDFLRAEGNSQTLLQEQIKTGERGQIWHALTEL
                     RYIFRHSLLREAAYNMQLRSRLSQLHALIAQAIEQLHTADLQERYADLAFHYEQAGIV
                     GKTCEYLLKAADYARRNYQNKQALHYYDKLIALLPRKAEPEQVIKAHLRRGKILEQTG
                     RWEECRQSYARALRTAQRTHQEAWLGQAHNHLGQVLLLLGDYNGAQKHFEKASELAEV
                     QNDVIGQARVYGNLGNLFFRLGQYNRAKDYYTRSIAAARRSDPAAVGAQTVAHFALAC
                     MNQGDFDNAVNIIQEQIPHSRILGDKPGMTSLYTNLGIIYFEKGDYDAALDSYRQGIA
                     LSEELGNKMFTAINTGCIGSVYERRGEYSKAMQHFVRDLELVEEMGDKQGIAIALTLI
                     GELLSYMGEFGQAIDYLQKSIMICEELRYQKGLAKAINTLGDVFFYTGQHQRAIEYYD
                     RAIEVSREIDAKAVLGISLVEKGAVLVETGQTEDLENIVSESLQLALSLGHSELEFEA
                     RLLAAQLLNLQNRREAAIQSLQDLAQSPLPPEQQAALYYQLHRMSPDQDNLRWRALEL
                     YQGLCDQTPKYLFKARLSALGSKDDGEG"
     gene            12060..>12750
                     /locus_tag="PK198_08195"
     CDS             12060..>12750
                     /locus_tag="PK198_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38755.1"
                     /translation="MKALHILSNLSGKTLFLPVLMVMLALTTSLAQAPANDLCSGATP
                     IACGQTLTGSTLSATIDQAPACGLSLPKYGVWYSIAGTGGDITLSTCGGANWDTQIGV
                     YSGSCAALTCVAGNNNFCGRQSTVTFSSVSGTTYYIWVTGVIDARGNFSLSATCANVA
                     PANDLCANAQALKKFGERYLTMKSLDFAQQDLYANVKDPDAIIHALMSSLAQRGSGQI
                     ENEGIGADAFFD"
BASE COUNT         2818 a         3266 c         3477 g         2827 t
ORIGIN      
        1 cgggctgcaa gcctcaagct acaagcggca agctacaagc ggcaagccgc gggttgcgat
       61 ctgcgggttg cgtgaggggg cggagggagg tgacgaccaa agcacccgac ggtggcgcct
      121 tttgcctgcc cgttcctcca ggataaaaga gacagcgcca ccggcacgcc caaataatca
      181 ggcacatcat tacatcatgg aatacgacat cattttactg acggaaacgc gcttcactgc
      241 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtacctgc gccagatatt
      301 gaccgacgac cgcctgctgg ccgatgccct gcgcgcgcat gggctgcggg tggcccgcaa
      361 agactgggcc gatcccgatt tcgactggac gcagacgcgc gccgctgtgt ttcgcactac
      421 ctgggattat tttcaccgct tcggcgagtt ctcggcctgg ttggaacggg tgacgacgca
      481 gacgcaattg ctgaatgcac cggagaccat tcgctggaac atggacaagc actacctgct
      541 cgatctggag gcgcagggca ttcgcatacc gcctacgcgc atggtgcata agggcgaggt
      601 cacgacgctg gaggctttgc accgcgagac cggttggcag gggcaggaaa cgatcctgaa
      661 acccaccata tcgggcgggg cgcggcatac ttaccggctg aatccagact cttgggcggc
      721 gcatgaggag gtgtttcagc agttgatcgc gcaggaggac atgatgctgc agccgctcca
      781 gcaccgggtg ctcagcgagg gcgaaatgtc gctgatggtg atgggcgggc gtttcacgca
      841 cggggtgttg aaaatagcga aaccgggcga tttccgcgtg caggacgatt ttggcggcac
      901 ggcgcatccg catacgcctg cgcccgaagc catagcgctg gcggaacgtg cggcggcggc
      961 ttgcagtccc ttgcccttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ggagaaaatg
     1081 cttcgggcga agtctgtagc ggaggcgatt gcgggggtgc tggcataggg tggagagcag
     1141 agagcctcta ctcgcagcca gatgagggag tgcctgcgcc tgccctcact tccggttgta
     1201 ctctgccgag agcacctctg tatcctgccg taccaattcg agcatagcgg gcgggttgat
     1261 gaccttggtg ctgtagctca cgatgatctt gttgttggtg ggccacactt ttttcaggag
     1321 tttcatgtcc tgtttggcgt agttgcgcag ccaggcatcc tgatcggttt cttcgcgcaa
     1381 ctccaccacc aactggtttg caatgacgcc cgccggcaat tggtagtcgn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nggttcgggg cgggtttctt tgccgagtat agacttggag cgctctgctt cgtggtaggt
     1561 gatgttcacg gcgggcaggt cgggaaactg gctgcgatat acgtccggat attgccacag
     1621 atctgccttt ttgaaagcca gtaagagcga ctctaattcc gatttgggca ggcgcttcag
     1681 gtagaggccc attttgtcgg tgtgtcgttc gcctttgtaa gcggccacgc cattactata
     1741 tatggtgagc gtaaacaccg ggcagcgccc gaaacaagcg cctttgctca tttcgatgac
     1801 gggagccagt ttgtcggtgg ctaccagcga aagcggcgga ttgcaggacg tgatcgcggc
     1861 ggccaatacc agcacaggcc agagtaaaac agggtttttc ataatgattg tgttgttggt
     1921 gaataagtgt tgttttttac tcaatgacta ccttcccact ccaaagcact ccctcccccc
     1981 cgatgcgcag cacatacatc cccgcggaca atcccgacac atcaacagat tgaaggccgg
     2041 cgtggatcag taaggttccc tggcgcagcg gcgcgcccca catcgaaacg agctgccaat
     2101 ggaagcgctg tccggccggc gccatggtgc gcagcagcag gctttctcct gttggatttt
     2161 gcaacagctc aaaaagggcc tcctgttgag gcgcctgcac caccgaaacc gggctgcaag
     2221 cctgcgccgc cagcatcagt ccgggccggg ccgcagcgat ggccgcccaa acccggtttt
     2281 tttgtccgtt gctgaacata gccatgcagg cgtcgtcggt gtagttcata aaattcataa
     2341 acatatcggc gctgccgcaa ctgatctgag gatgcgtggg gcactcgccg aggtagcttc
     2401 tcgattgcag gggcgtatcg gccacctcat cgctttgggt gcaggagccg gtttgacccg
     2461 gtccccacaa gtggtacagg ttgaaataat gccccagttc gtgcgtcagg gtgcggccca
     2521 ggtggtaagg ctggttggag gccgcgctgc ccgtcgttcc tacaaacggc ggactgatga
     2581 tgatgccgtc ttccgctgct gcgcccatgc cgggaaaact cgcctcggca ggaaaaaact
     2641 gccggcgcgc cacccacacg ttgacatacc tgccggtatc ccaggcatcg ctgccgccct
     2701 cggcggtgta gcacacgctt ttgcgcccgt tggcgatgcg gtcgcccata aacgggaagg
     2761 aggtttgccg gcgcaggatg ccgttggtgg aagctccgtc ggggctgcgg cgggcgagac
     2821 aaaaattcaa ttccatgtcg gcagccagcg actgaaaaag ggacggaatg aggcccgcgc
     2881 tgttgttggt tctgcgaaag tcccggttca aaacctccaa ctgagagagg atttgctcat
     2941 cggaaatatt ctcctcggcg gtattccaga caacgtgaaa caccaccgga atatctatcg
     3001 tgctgcgggt ttgaacggcc tgctgaagct gaatgaaatt ttccagatcg gcccgggctg
     3061 cctccgcgtc aggatgctgt tgcaaatagg ccgccagccg ctcctgcgaa acgcatcgct
     3121 gcgcccacag ttccgacgaa gacaacaacc cgacaaggca tccgatgagt ataagccgca
     3181 gtgccatgcg tcattgaccg aggagttgca tcactcgtgt catgttggga gagaggatga
     3241 tatccgtttg attcacaacg gctttgatgc caaaggctgt actgaattga gggccttgcc
     3301 ctgccgccag gattttgccg ggctgtacgc cgcctgccgc cagaaaacgc cccactgacg
     3361 atgcccgctg tatgccatat tcccagttca cgtcggaagc gccgtagctg tcgccgcgcc
     3421 ccaccaccag cacttccaca tcgggaaagg aagccaatgc ggaggctatt tgctgaatga
     3481 cttgttggcc ctgcgttccg ggctgtattc cgccgtcggt aaagagcagg ctgtgctgga
     3541 gcgagacgtt caatcggttg ttgtcggtgc gattgatttg cacggctgat gctccgtaag
     3601 gcgccagtat gcggccgagg ttttgctgca gctggttcat ttgtctttgt tgggcatcca
     3661 gcaggttgtt cagttcctgt tgagaggccg aggtcgtgcc tttggccgtc atttgatcgt
     3721 aagtttgcac ggcgccgagg cgttcttctt tcgactgaat ggcgtactcc atggccgccg
     3781 cttcgcgttg tttgcggtcg agttcggcct gctgggcggc gaggcgctcg gtgaggcttt
     3841 gcttctcgta ggcgctgctg gagagtacgt cgcggttgga ggtcactacg cggttgtaat
     3901 ccgtcagcaa ttcgttgtaa tttctatgca gggcggcatt ggtgatgctt gcgtcgttga
     3961 tggccatctc ggctttattg agttcggctt cgaggttgcg gttgcgctcg tacagggtat
     4021 tgttggctaa acgaagcgag tcggcggctt tggattcctg tttgtaataa ttgcgggatt
     4081 cggtcagctc ttcgtaggat gctttggaaa tacagcctga aagcagggcc aatacggcaa
     4141 tcagtggtaa taatctcatg gttgaaatgt tttggaacaa acaaaagtaa ggctttcctg
     4201 caatgcaaac aacattaacg ttccgattga aaaaatactg ccggagatat taataatttc
     4261 gccccctaaa aaaacaacat gaacaagatg ataatagtat ggatgacggc gctgctgctg
     4321 agcgcttgtg ccggtgaggg aggcaattcc aaggccgttc gccagcaagc caaagaagcc
     4381 attgaggcag gaacggcaac gcctgccgcg cccgctgaag aagcaagccc gcaaataacc
     4441 ggcaacattg tgatgacgtt gcccaaaggc acgctggcag cgggcgctac gcaatgtttc
     4501 gaggtaaaag tatcgggttt taaagacatt ctggccacgc aatacacgct caactgggac
     4561 tctaaagtta tgaatttcaa agaaatcaaa gaattcaagt tgccgtttat gaccaaagaa
     4621 aacttcggca tcgcgctgac acccaacgga aaactaagct ctgtgtggat cgaaaactcg
     4681 ctcaaaggag ctaccatccc cgacggcggc gctatttacc aactgtgttt cgaggcgacg
     4741 ggcaaggccg ggcagtcttc ggaactatgg gtatcgggca cacccactgc cattgaggta
     4801 gtgaccaaag gcgatcaggt gtggggcatt aattcggtga aagggaaaat tacggtagag
     4861 tagatcgggc ctgcggccgt ggggcggttt gtgagagagt aggtcgtata attttccctc
     4921 ccgactttta cccccgtcgc accaatcgct gataatccaa aaagtagata agcttgatgg
     4981 agaggctgtt gttttgcggc gcgtcgaaga gattgtccaa gttggaaaaa taatcctcgc
     5041 cggccagcgc atcggcgtgc aacaccgagt ttttccaaat cacaaacaaa tcgctgccgg
     5101 gcgcaaatcg ccagcggaaa atcatgtcca cattgaaggc attaaaattg gcatcgcgct
     5161 cggggttgtg gctcgtttcg cccaagttac cgtcctccgt cagcaggtgg taccggctgt
     5221 actctacgtt ggtccagtag tgccgggcgc gcagggtgag ggctgtattg ggattgaagg
     5281 tgtagctggt attgaacaca ttttccaccg tttgaatgag acggcgacca aaaatgacgt
     5341 cggtgacgcc ggtggccggg ttctctattt tgtcggcaaa gcccacatcg ttgtaccagt
     5401 gtccgtagta tgtactgagg ctgagcgtga gtttgtcgtt gacgcgatag cggggcgata
     5461 tgctcgtatt ccagcggtag cggccctcgt cggcaaaggt gcggaagttg ctgttcatgg
     5521 aaatgcggat ctgcttccta ttgtcggtgc cgaaccagaa tccgacgttg ttgttggtgg
     5581 gaaaatgata aaatctgccc ggcgtgcgcg cttcaaaata gtcaaaggtt ctgacaggtt
     5641 cgtgataggt aaagacatta aaattccaaa aggttttcgt ttgcgcccat gcccagaaat
     5701 tgatgccgaa atctgtaaac acattgggct tatacaggcg gttgtagttg acataagctc
     5761 cgccgcccgc cgagttgaat ttgccgaaag gctggtattg ttggtagttg atttccgcgc
     5821 caacactgcg ttcgttgttg ttgtacaaaa agcccagatc gttgggatta taggtgtcgc
     5881 tttcttccac atacgacagc ccgccctgcc acttgccgct gattttgccg atgccgatgt
     5941 tccaggaatg ccccagatca acctggccgg ggtggtacaa ctggctcaat accccgcgac
     6001 cgcgcagccc ccaggagttg cctttgttgc gaaaatcgaa caccgccgcg ctcacattgg
     6061 cgtcataatc ggcgcccgaa cgccaggccg tagtattgac cagcgacaca aaggagttgt
     6121 ttttcaggtt ttgatcggct accatgacgg tgtagttcgt caaggggtcg gtttccactt
     6181 cgcgctctaa gccctcggca ttgcgaatga cggcatggct gcgcgccgag gtggcattga
     6241 acaggcccaa acccagccct ttggccgtgc ggcccgatat tttgctggcg ttgatgagct
     6301 tggcctgttg cggattggag actatggttt cgccttcctg taattgatct tctacgtccc
     6361 aatatttgat gggcgtgccc ccgatgcggc gcgagtaaaa caggccgcct ttgttgaaca
     6421 gctcggtgcc ttctgtgaaa aactgccggt tttcgttgaa ttgtacctcg aacggcgaaa
     6481 gattgagtat ctgattgtcc gactgcgcct ccccgaagtc gggaatgagc gtcatatcaa
     6541 gggtaaaggc gtcgctgatg ccgtacttca catccatgcc gccgttgaac gagtagccga
     6601 ggctgttttt ggggtcgctg tttttatcgt ggtaatgctg gccgtacacc gcaacaaacg
     6661 gagtcgcctg aaggcgcagc ggcgctttga tgttttcaaa cccctcaatg atgccggact
     6721 gaatgaggaa accgttgacc tgcgggtcta tgtagttcca gaagctgttc tcattgaggc
     6781 gcttaacact tctcccgaaa ttgatgtgcc agctttggac cggcgttttg ggaaagcgca
     6841 aggccgcaaa aggaatgtga atttcggccg tccagccctt gtcgttgatg atggatttgt
     6901 tgtaccacac ggcatcccag ttgatgtctt cgccttgggt gacgatctgg ttgccgtcgt
     6961 tgtcgccgcc tgaaacggga gcaattttca agtccacctg aacgccggcg ggcgtcacca
     7021 aaaagcccag cccgttgagg ccgtcctgat aggcgtcaat aaaaacgccg aacacgtccg
     7081 tattgccgag gcggtcgcgt tgggtgagtt cctgcaggat gctgtcgggg cgcgggtcca
     7141 acatttgtgc gccgatataa atgcctgagt tatcgtaaat tactttgatc tgagtggtaa
     7201 aagccgaagc tgcgccggga acaggagaag tttggacaaa gtccgcagct atggcggcat
     7261 ttgcccaaac ctgctcgttc aatacgccgt ctatcgttat gcgttcctct gttcggaggg
     7321 cagtcatgcg gcgtttttga ggcgcggacg actgagcaaa agcagctact ccggcgagga
     7381 gcatagcgat aaggagaaag gcgttgcgtt tcattgggtg acttcttaat ggtaaccggc
     7441 taaaaaattc gagttttccg ataaagggaa atcccaaata ccggcaaccg gaacgaacag
     7501 actgtgcttt tttgtaaagg ttgcacaaaa ggggcatcaa aaaaactgtt tttgcccgct
     7561 acaaaagggc atcaaaaaaa cagattttca ttttcgtcaa aggattagat aagtaaacgc
     7621 ttcattttaa gtgccttatc tgatcctttg gccttaataa aatgaaaaac tgtttttgcc
     7681 cactacaaaa gtagcgagaa aaaggcaggg cttgttattt ttgcccggat atggggcaat
     7741 gaaaaagcag attttcattt tcgccccgct accatgtcat gccggtgaaa aatcttattc
     7801 cacatttcat tcaggatcaa agtcttgcag gcaaaaccta cgggcgcatg caggcttata
     7861 ccatgtttgt ggacatgtcg ggcttcacgg ccctcaccga aaccctcatg gcgcaggggg
     7921 cgcgcggcgc cgaacaactc tcgcaggcgc tgaacgacat ttttagtcca ttggtgcgcc
     7981 tggtgtatag ccggggcggc ttcattccgt actttgccgg agacgccttc acggccattt
     8041 ttcctgccaa cagcccccgc gttactgcta ccggattcat acaaacggcc atcatagccc
     8101 gcgatatgtt tgccggtaaa gaacttcatc ttggcgcctt ttctatctct atcaaaatcg
     8161 gtttgtcgtt cggcaatgtg gagtggggca ttgtaggcgg caaccggcaa gcgtattatt
     8221 ttcggggctt gccggtagat cacagtgcgc aggggcagtc catggccggg aaattggaaa
     8281 ttgtggtaga tgccgctttg tgcaagatgc tggatgggca gggtttttct ttgtcggaac
     8341 tcagcggcgg gttcaaaaaa ctcaacgata cgcctcccgt cagacttcat tctggcaaag
     8401 ctgccgaact gcccgaactg aacgaggcgg tcggcttgcg gtttttgccc cgcgaagtgc
     8461 tcgatttcaa ccaaagcgga gaattccgca cggtcattgc ggttttcctt tcttttgacg
     8521 gcgtggacaa ccacgaactg ctcgaccggt ttgcaggcat cgcattggaa caggccgaca
     8581 atttttcggg ctattttaaa gaaattgact tcggcgataa aggcggggtc atggttgtct
     8641 ttttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct gccaatatgc
     8821 ggcggtgggc aatcgggtga atctggctgc gcggctgatg agccatgccg attggggcga
     8881 ggctttggtg gacaatgaac tccgcaaaaa ccggcagtat cgtttctacc acaaaggcga
     8941 tattaaatat aaaggtataa aaggcaacgt gcctacttat ttgctgttgg gtcgcaacct
     9001 tgagtatcaa catagttttt cggggccttt ggtggggcgt acacgcgaaa tggagcaact
     9061 cctccgcttt tccgagcctt tgaccaaagg ggtaagtccc ggcacggtcg tcgtttatgg
     9121 cgaggccggc tccggcaaaa gcaggctttg tttcgaactg cgcaattctt tacaaaaaag
     9181 cgatctggca gagtggtaca cctgcgccgc cgatcagata cttcaaaagc cgctcaacgc
     9241 tttcctcttc tttctgagaa attacttcga ccagtcgctc gaagcggcgc cccgcgaaaa
     9301 tcgccggaga tttgaagccg gttttgacgc gctgatagca gagctggagc aaaaaaaagc
     9361 ccccggatac gagttcatgc aacgcgaact gtggcgcatc cgatctgtgc tggccggcct
     9421 gctgggcatt cactaccccg attctctgtg ggaacaattg gatgccaaag gccgctatga
     9481 aaacaccacc caggccatcc tgcacctgct caacgtggag gcagccctcc agccctttgt
     9541 attggaaata gaagacgcac actggctcga cgatcaatcc gccgaactgc tgcacgagat
     9601 cgtccgccgt ttgcacaaat ggcccatttt gatattggtc acctcccgct accgcgacga
     9661 cggtaaagaa ccggaaatcc tgcccgaagc tgcgcaggcg gaacaattag tgattcggct
     9721 ttctccgttg gaagccgagt cggtgcggcg ttttgccgag cttcacctgc aagggaaaat
     9781 aagcccggga ttctacgagc agctattgcg ccacaccaac agcaatcctt tttatgtaga
     9841 gcaggttttg gagtattgcg tcgagcggaa tctgctccgg caggaaaacg gtgtgtggca
     9901 tgtcaccgcc gaagacattg acctctccgg ctccatacaa tctattctga ctgcccgtat
     9961 tgaccggctt tctacgctgg cgcgcgaaac ggtgaaagcc gctgcggtga tcggcagaga
    10021 atttgaagtg cccgtgctca atgaggtcat gattcatcag gaagacttcc tgcgggccga
    10081 aggcaacagt caaacgctgc ttcaggagca gatcaaaacc ggcgaacggg ggcaaatctg
    10141 gcatgcgctc accgaattga ggtacatttt caggcactcg ctgcttagag aagccgccta
    10201 caacatgcaa ttgcgttcgc gcctcagcca attgcacgcc ctcattgctc aggccataga
    10261 acaactgcac accgccgact tgcaggaacg ctatgccgac ctcgcttttc actacgaaca
    10321 agccggcatt gtgggaaaaa cctgcgaata cctcctcaaa gccgccgatt atgcccgccg
    10381 gaattaccaa aacaaacagg ccctgcacta ttacgacaag ctgattgccc tgttgcctcg
    10441 caaagccgag ccggagcaag tcattaaggc gcatctgagg cggggcaaaa ttctggagca
    10501 aaccggccgc tgggaagagt gtcggcaaag ttatgcccgc gcgctccgca ctgctcaacg
    10561 cacgcatcag gaggcatggc tggggcaggc acacaatcat ttggggcagg tcttgctgct
    10621 gctgggagat tacaacggcg cgcaaaaaca ctttgaaaaa gcatccgaac tggccgaggt
    10681 tcaaaacgac gtcatcgggc aggcccgcgt gtacggcaat ctgggcaatc tttttttcag
    10741 gcttggccaa tacaatcgtg ccaaagacta ctacacccgc agcattgcgg ctgcccgccg
    10801 ttccgatccc gccgccgtgg gcgcgcaaac agtggcgcat tttgcattgg cctgtatgaa
    10861 ccagggagat ttcgacaatg ccgtcaacat cattcaggag caaattccgc actcgcgcat
    10921 actgggcgac aaacccggca tgacctcgct ctacaccaat ctgggcatca tctattttga
    10981 aaaaggcgat tatgatgccg ccttagacag ctaccggcag ggaatagccc tgagcgaaga
    11041 attgggcaac aagatgttta ccgccatcaa taccgggtgc atcggcagcg tatatgaacg
    11101 gcgcggcgag tactccaaag ccatgcagca ttttgtgcgc gatctggaac tggtggaaga
    11161 aatgggcgac aaacaaggca ttgccatcgc cctgacgctg atcggcgaac tgctcagcta
    11221 catgggagag tttggacaag cgatagatta tttgcaaaaa agcatcatga tttgcgaaga
    11281 actccgctac cagaaaggcc tggccaaagc cataaataca ttgggggacg tattttttta
    11341 cacagggcaa caccaacgcg ccatagaata ctacgatcgg gccattgagg tcagccgcga
    11401 gattgacgcc aaagcagtat taggtatttc attggtggaa aaaggagccg tactggtaga
    11461 aacgggccaa acggaagacc tggaaaacat tgtatcggag tccttgcagc ttgccttgtc
    11521 tctggggcac agcgagcttg aatttgaagc gcgcctgctc gctgcgcagt tgctgaatct
    11581 ccaaaaccgg agagaggccg ccattcagtc gcttcaagac cttgcccaaa gccccctgcc
    11641 tcccgaacag caagcggcgt tgtattatca actacaccgc atgtctcccg atcaggacaa
    11701 cctgcgctgg cgcgccctgg aactatacca aggcctctgc gaccaaacgc ctaagtactt
    11761 atttaaagcg cggctgagcg cacttggatc gaaagatgat ggggaaggat aaaaaaaata
    11821 ttttttttgc gacaataatt gcacatttta aaaaaagcta tatctttacc ccgcaaatta
    11881 atctcgtgac ttccgacggc cttaccaaca tccccctccg gtaatcgtcg agaagcattt
    11941 tcaataagag gcatacctga cagctttgag attgctatcg cgcatccgcc gtgatagagc
    12001 ataattattt tgctatagaa tcatctttat ttgttcacac ttaaattcgg cttagacgaa
    12061 tgaaagcact tcacattctc tccaacctga gcggaaagac gttgttcttg cccgttctca
    12121 tggtcatgct cgctctgact accagcttag ctcaagcgcc tgccaatgac ctctgttccg
    12181 gggcaactcc cattgcctgc ggccaaactc ttaccggcag caccctcagt gctaccattg
    12241 accaggctcc tgcctgcgga ctttcccttc ccaagtatgg cgtgtggtac agcatcgccg
    12301 gtacgggcgg cgacattaca ctgtccacat gcggcggcgc caactgggac acgcagatcg
    12361 gcgtttactc cggttcttgc gcagcgctta cctgcgtagc cggcaacaac aacttctgcg
    12421 gtcgccagtc aaccgtgacg ttcagttctg tatctggtac cacctactac atttgggtaa
    12481 ccggtgtgat tgacgctcgc ggcaacttca gcctgtcggc aacttgcgcc aacgtggctc
    12541 cggccaacga cctgtgcgcc aatgcacaag ccctcaaaaa atttggtgaa cggtatctga
    12601 cgatgaaatc gctggatttt gcccaacaag atttatatgc caatgtcaaa gacccagatg
    12661 cgattattca cgcgctcatg tcatcattgg cacaacgcgg gagtgggcaa attgaaaatg
    12721 agggcattgg tgcagatgcg ttctttgacc
//
LOCUS       DAORLF010000074        12706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2634_length_12706_cov_2.573947, whole genome shotgun sequence.
ACCESSION   DAORLF010000074 DAORLF010000000
VERSION     DAORLF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2634_length_12706_cov_2.573947"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1045)
                     /locus_tag="PK198_08200"
     CDS             complement(134..1045)
                     /locus_tag="PK198_08200"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRF38756.1"
                     /translation="MSRQPIWTGVYPAVTTKFTAEGALDIPAFMHNIRAQVEAGAQGI
                     IIGGSLGESSTITHEERLILLAAVLDEYGEQVDVILNVAEGSTARAIELTQKAEAAGA
                     HGIMLLPPMMYKPTDDEVAQFFLDVARATQLSILVYNNPVDYKIEITLDIFEQLAHTP
                     NIAAVKESTRLTSNVIRMRNRFGDRFKILCGVDTIALEELALGADGWVAGLVCAFPKE
                     TVALYRYVKAGRYAEARALYQWFMPLLELDVSPQLVQNIKLAEAFTGLGNENVRPPRR
                     PLQGAERERVIAIIEAALQQRPGIGEL"
     gene            complement(1270..2055)
                     /locus_tag="PK198_08205"
     CDS             complement(1270..2055)
                     /locus_tag="PK198_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF38757.1"
                     /translation="MNLNLTGKNALVGGSSKGIGKAIAMELAELGANVTLVSRSPELM
                     TTITRQMKRRPGQDHDFLAADFTNRADLRTKVAALVSAKPIHILVNNTGGPPGGPILD
                     AAPEEFEVAFHNHLIINQMLAQLVVPGMLRENYGRIINIISTSVKAPLDDLGVSNTVR
                     AAVANWAKTLANELGRHGITVNNVLPGLTDTERLHDLIQKKAVATRSSYETVANAMRS
                     SVPAQRFAEPHEVAAAAAFLATPAAAYINGTNITVDGGRTRNL"
     gene            2116..2634
                     /locus_tag="PK198_08210"
     CDS             2116..2634
                     /locus_tag="PK198_08210"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRF38758.1"
                     /translation="MPIAQPFNLYQWIDDNRALLQPPVGNHQIYKANDDYIVMVVGGP
                     NGRKDYHYEDGEELFFQLKGDMSVKIIDENGQPQTIPIREGEMFLLPPRVPHSPQRPA
                     GTVGLVIERYRKPGEIDKLMWFCENCHEKLYEAGFELQDIGTQIKAAIQEFMASEDLR
                     TCKHCGTVMEKM"
     gene            2646..3422
                     /locus_tag="PK198_08215"
     CDS             2646..3422
                     /locus_tag="PK198_08215"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRF38759.1"
                     /translation="MKKTYFASDFHLGVPGRLSSAEREKQIVRWLDQCSRDADAIYLV
                     GDVFDFWFEYRTVMPKGHVRLLGKLAELRDGGLPIYFFTGNHDMWVFGFFENELGIPT
                     FRSPIVREIQGSTFFIGHGDGLGPDDYGYKRIKKVFASRLNQWIFERIHPNFGIGLAQ
                     FWSGKSRSLYPEAPAFLGEDKEWLVAYANRKLDTVPADFFVFGHRHIPIDFTLKNGRS
                     RYINTGEWLYACSYAAFDGERMELCFFENEKGYQGRGVSV"
     gene            3674..4189
                     /gene="ribH"
                     /locus_tag="PK198_08220"
     CDS             3674..4189
                     /gene="ribH"
                     /locus_tag="PK198_08220"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRF38760.1"
                     /translation="MASALKNLSAYNESDMPSAAEMSFGIAVADWNAHITHALYEGCV
                     EALLKHGALPENIHTMQVPGSFELPAACRLLLSSKKMDAVIALGCVIQGETNHNEYIN
                     HAVANGLVQLSLATGIPCIFGVLTPNNEQQALDRAGGVHGNKGVEAAATAIRMAALKK
                     ESGTPKKRIGF"
     gene            complement(4197..4883)
                     /gene="rpe"
                     /locus_tag="PK198_08225"
     CDS             complement(4197..4883)
                     /gene="rpe"
                     /locus_tag="PK198_08225"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRF38761.1"
                     /translation="MIRNHLIAPSLLAADFTRLERDVAMVEQSRADWLHLDVMDGRFV
                     PNITFGMFIVQAIRKMSAKPLDVHLMIVEPERYIEPFRAAGADVITVHYEACPHLHRT
                     LQQIRDTGAKAGVAINPHTSVELLDDVLEDLDLVCIMSVNPGFGGQKFIYRSIPKIAR
                     LRQMADERNLYPLIEVDGGVGLQNAEKILQAGADVLVAGSSVFGADNPTEAIARMKDI
                     GQEARQPFLV"
     assembly_gap    5105..5119
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5255..6106
                     /locus_tag="PK198_08230"
     CDS             5255..6106
                     /locus_tag="PK198_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRF38762.1"
                     /translation="MKKSFQLHKTSNMMNEFLRACLLFTALLLQLSLSAQWTWQETGN
                     ASYYADKFHGRSTASGERYNKNELTAAHRSLPMGSIVRVTNARSGQSVEVRINDCGPH
                     RSDRVIDVSKAAAKKLHLIADGVAPVIVELVEPGEGHCACDRKKQWDPTPWRAKETVI
                     TKETEVTATTRPEQTPPAPRKEKTQPGSVAETALESPVVQEPPSTGIVLQLGAFGSKD
                     NANNFAQKINKQGFSDVFVKEFPQGDKTLYRVYRGAFSSRTEAETAQKELKGKAGIDG
                     VITELKL"
     gene            6177..6932
                     /locus_tag="PK198_08235"
     CDS             6177..6932
                     /locus_tag="PK198_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRF38763.1"
                     /translation="MWNKQKLFSAIIALFSLTAVHAQWSETGIAGYYADNLHGKKTVG
                     GELYDKYALTAAHHNLPIGSRILITRLDNQQSVEVRVNDCCLAVKGRIVSLSRAAAEK
                     IGLIQAGTAQVRIDLVSRGDGKTCAGDKPVAAYSKEAEKVTPKGGSAPAVEKGPVAPG
                     TYRADALKPIQSGFGVQVGAYSDKANAEARVEEMRKKGFKDLLVTYKGADQKIPYKVI
                     IGPFDTAASAAAYNKNLAAKHKVKGHVVSLKEG"
     gene            7153..8142
                     /locus_tag="PK198_08240"
     CDS             7153..8142
                     /locus_tag="PK198_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HRF38764.1"
                     /translation="MLSAIENPQIQQILTLPSDSDAGWRRDLERLRNGEVNLSRKNIG
                     ENSIKAIQRLFIFLGYSTASSGAFSIDGDFGRGTNRAVAQFRFENNLSTTITRATLCY
                     PCTWQTARQAIVAIPDTLLDVPSLEKLLSNAVEAIANGQVNCGNFDVAVEHLNGLHQN
                     RLMTCKQIWERYGAQAKTAAAQIETELRVAIRPEWILSIIRQETGGVVRPRFEQHLLT
                     KMNAQMPNTDLAELRYRSMSFGLGQILGTNFRTVGAASARAMFTSPLDEQLLFVGRFL
                     AKNREMAKVVVKKQPVSEDFHTIGKYYNGPGYAKHHYHESIERWFKEFRILIG"
     gene            complement(8174..>8649)
                     /locus_tag="PK198_08245"
     CDS             complement(8174..>8649)
                     /locus_tag="PK198_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38765.1"
                     /translation="SFCAGISLHRLVFTETLTTRWGSDWPFNGEFTTYKTTSKLYDAG
                     CTVMWRRSISQRLHRIAPAVGFTASRQWGPVTTLRISGRGDDLTLDHYDLPRFNLSAL
                     LGIQCTLAQWERNSIHIMPLYAYALSPVKSTSEVGATATYYPHYFSLQVAFQHKL"
     assembly_gap    8650..8725
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9015..9082
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9083..>9581)
                     /locus_tag="PK198_08250"
     CDS             complement(<9083..>9581)
                     /locus_tag="PK198_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRF38766.1"
                     /translation="KVVPGQGYLTDHYNPMTKTVSLSEPVYHERSIAAAAVAAHECGH
                     AVQHAEAYAMLQVRSAIVPVVQISSSLQQFILIAAFVTINTMPQIMLAAIVVFAVTAL
                     FSVVTLPVEFDASRRALVWLENSRTTQGAEYDGAKDALKWAAMTYVSAALSALVMLAY
                     LILRYM"
     assembly_gap    9582..9594
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9595..9744)
                     /locus_tag="PK198_08255"
     CDS             complement(<9595..9744)
                     /locus_tag="PK198_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRF38767.1"
                     /translation="MIIAGVLSVLGFVVQQKLQSKFGKYSQIPLRSGMSGAEVAEAML
                     RYYNIT"
     gene            complement(9790..11067)
                     /gene="serS"
                     /locus_tag="PK198_08260"
     CDS             complement(9790..11067)
                     /gene="serS"
                     /locus_tag="PK198_08260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRF38768.1"
                     /translation="MLQLNFIRQNKEVVIKGLQKRNFSDDQLRIVDNILELDDNRKNI
                     QSESDALLAQRNALSKEIGDLMKQGRRDEAEALKAQVAAAKEKADQLGITLADTKNQL
                     EELLYQVPNIPHASVPEGKTADDNEVFKAWPQELPVLPESALPHWELAKKYNLFDLEL
                     GVKLTGAGFPVYRGKGARLERALINLFLDRATTAGYEEIAPPLMVNADTARATGQLPD
                     KEAQMYYVERDDLYLIPTAEVPVTNIYRDVILNESDLPVKMTGYTPCFRREAGSYGAH
                     VRGLNRVHQFDKIEIVRLEHPDRSYQALDEMLEHVSGLLNELELPYRVLRLCGGDMGF
                     TSALTFDFEVYSAAQQRWLEVSSVSNFETFQSNRMMLRFRDSGQNRLLHTLNGSALAL
                     ARIVAALLENNQTEAGIVIPAVLRPYTGFDIIN"
     gene            11166..>11540
                     /locus_tag="PK198_08265"
     CDS             11166..>11540
                     /locus_tag="PK198_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRF38769.1"
                     /translation="MSQRKWPIIAKIGTSALIYHLFYHLIFAPLLPAEGRAPCSKASS
                     QVPLLDNFNYLHHMYEISQLSDMLVPELREIADGLNIKGYRKLNKPDLIQSILDHQTP
                     AVAEVVAEPPAPEPEKPKRAMPS"
     assembly_gap    11541..11602
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11836..>12326
                     /locus_tag="PK198_08270"
     CDS             11836..>12326
                     /locus_tag="PK198_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF38770.1"
                     /translation="MPKYRQPEPELEPEEEEFEEYVELKGQHKKPQQPPRPVPAPAPA
                     PEPETPKMVQPIQTGPKFDIELDGVVEGLGVLEMMSDGYGFLRSSDYNYLSSPDDIYV
                     SPSQVKLFGLRTGDTVRGAIRPPKEGEKYFALLRVSKINGLDPVDVRERVPFDYLTPL
                     FPN"
     assembly_gap    12327..12475
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12476..>12706
                     /locus_tag="PK198_08275"
     CDS             <12476..>12706
                     /locus_tag="PK198_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF38771.1"
                     /translation="VANAIAKNHPECFLIVLLVDERPEEVTDMKRSVNAEVVASTFDE
                     PADNHVRVAGVVLEKAKRLVECGHDVVILLDSI"
BASE COUNT         2894 a         3311 c         3364 g         2754 t
ORIGIN      
        1 tgcgtgaggg ggccgagggt agtgacgacg caggaggagc gaccgaggca cccgaccccg
       61 ccgcctgata ctccctcctt tggagggtcg gggaggcggt agagcggggg cacgccccca
      121 aatatcttga cgattaaagc tcgccgatac ccggccgttg ctgcaatgcc gcctcaatga
      181 tggcgatgac ccgctcgcgc tcggcgccct gaagcggacg gcgcggcggt cgcacgtttt
      241 cgttgcccaa gccggtgaaa gcctccgcca gcttgatgtt ttgtaccaat tgcggactta
      301 cgtccaactc cagcagcggc atgaaccact ggtacagcgc ccgcgcttcg gcgtagcggc
      361 ccgctttgac gtagcggtac agcgccactg tttccttcgg gaaggcgcag accaagccgg
      421 ctacccagcc atcggcgccc agcgccagtt cttccagagc gatggtatcc accccgcaga
      481 ggattttgaa gcggtcgccg aagcggtttc tcatgcgtat cacgttggaa gtgaggcgcg
      541 tactttcctt cacggcggcg atgttgggcg tatgggccaa ctgttcgaaa atgtcgagcg
      601 tgatctcgat tttgtagtcc accgggttgt tgtacaccaa aatggagagt tgggtggcgc
      661 gcgctacgtc gaggaaaaac tgggctacct cgtcgtcggt gggtttgtac atcatgggcg
      721 gcaggagcat gatgccgtgt gcgccggcgg cctcggcttt ttgtgtcagc tcgatggcgc
      781 gggcggtgct gccttcggcc acgttgagga tgacgtccac ctgctcgccg tactcgtcga
      841 gtaccgctgc cagcaggatg aggcgttcct cgtgggtgat cgtgctgctc tcgcccagcg
      901 agccgccgat gatgatgccc tgcgctccgg cttctacctg tgcacggatg ttgtgcataa
      961 aggcggggat gtcgagtgcg ccttcggcag tgaatttggt ggtgacggcg ggatagacgc
     1021 ccgtccagat gggttgtctg ctcatgttat ttagttgtaa ctatttacca agccttccca
     1081 aagctacgag ctacgagttg cgagctgcga gtttgaggtt tgaaaaataa aaagcactca
     1141 ttttcaatat tagaatctga aatctcctcc aatagtaaat cttatttctg aacgaaaaac
     1201 aaactcatag ctcatagctc atagctcgcg gctcgcagct tgcagagtag ccaaatagtt
     1261 atattgaatt tacaaattcc tcgtccttcc cccgtccacc gtaatattag tgccattgat
     1321 gtaagcggcg gcgggtgtag ccaagaaagc ggcggcggcg gccacttcgt gcggctcggc
     1381 aaagcgctgt gccggcacgc tgctgcgcat ggcattggct acggtttcgt aagagctgcg
     1441 ggtggcgacg gcctttttct gtatcagatc gtgtaggcgc tcggtgtcgg tgaggccggg
     1501 cagcacgttg ttcacggtga tgccgtggcg gcccagttcg ttggccaatg ttttggccca
     1561 attggctacg gcggcgcgca cggtattgga cacaccgagg tcgtccaaag gtgctttcac
     1621 cgaggtagaa atgatgttga tgatgcgccc gtagttttcg cggagcatac ccggcacgac
     1681 gagctgcgcc aacatttgat tgatgatgag gtgattgtga aacgccacct caaactcctc
     1741 cggcgctgca tccaaaatgg ggccacccgg agggccgccc gtgttgttga cgaggatgtg
     1801 gatgggcttg gccgaaacca aggcggctac tttggtgcgc aagtcggcgc ggttggtgaa
     1861 gtcggcggcc agaaagtcgt ggtcttgccc aggccggcgc ttcatctgcc gggtgatggt
     1921 cgtcatcagt tcgggcgaac gcgatacgag tgtgacattg gcccccaact ccgccaactc
     1981 catggcaatg gccttgccga tgcccttgct gcttcccccc acgagggcat tttttccggt
     2041 gagattcaaa ttcatgtggt caaatggctt tgttcgggca agattagcat atttttacca
     2101 aaaaatttca gccttatgcc aattgcccaa ccattcaacc tataccagtg gattgacgac
     2161 aaccgcgcac tgctgcagcc accggtaggt aatcatcaga tttacaaggc caacgacgac
     2221 tacatcgtga tggtggtggg cggccccaat ggccgcaaag actaccacta cgaagacggc
     2281 gaggagcttt tttttcaact caaaggggat atgagcgtca aaatcattga cgaaaacggc
     2341 caaccgcaaa ccatccccat ccgagaaggc gagatgttcc tgctgccgcc ccgcgtgccg
     2401 cattcgccgc agcgcccggc cggcaccgtg ggcttagtca tagagcgcta ccgaaagccc
     2461 ggcgaaatag acaagctgat gtggttttgc gaaaactgcc acgaaaaact gtacgaggca
     2521 ggcttcgaac ttcaggacat cggcacgcaa atcaaagcag ccattcagga atttatggcc
     2581 tccgaagacc tccgcacctg caagcactgc ggcacagtca tggaaaaaat gtagctcaac
     2641 gaggcgtgaa aaaaacctac ttcgcttccg attttcacct cggtgtgccg ggccgcctca
     2701 gcagcgccga gcgcgaaaag caaatcgttc gctggctcga tcagtgcagc cgcgacgccg
     2761 acgccatcta cctcgtgggc gatgtgttcg acttttggtt cgagtaccgc accgtcatgc
     2821 ccaaaggcca tgtgcgactg ctgggcaaac tggccgaact gcgcgacggc ggtctgccca
     2881 tttacttctt caccggcaac cacgacatgt gggtgttcgg ctttttcgag aacgaactcg
     2941 gcatacccac cttccgcagc cccatcgtaa gagaaattca gggcagcacc ttcttcatag
     3001 ggcacggcga cggcctcggc cccgacgact acggctacaa acgcatcaaa aaagtattcg
     3061 ccagccgcct caaccagtgg atcttcgagc gcatccaccc caatttcggc atcgggctgg
     3121 cgcagttctg gtcgggcaaa agccgctccc tctacccgga ggcgccggca ttcctcggcg
     3181 aagacaaaga gtggctcgtg gcttacgcca accgcaaact cgacaccgtg cccgccgatt
     3241 ttttcgtctt cgggcatcgc cacatcccca ttgattttac cctcaaaaac ggccgctccc
     3301 gctacatcaa taccggcgag tggctctacg cctgctccta cgcggccttc gacggcgaac
     3361 gcatggagct ttgttttttt gaaaatgaaa aggggtatca ggggcgaggg gtgagtgtgt
     3421 gaggggtgag tgtgtgaggg gtgagtgggg cgtgccggtg gcgctgtctc cctcctcttg
     3481 gagggtcggg gcggcaaaag cgccaccgtc gggtgcctca gtcgctcctc ctgcgtcgtc
     3541 actaccttcg gccccctcac gcaacaggca agagctgttg gctttagccc ggcaactaca
     3601 agccaaagcc aacagctcca cagcccgtac tcgcggctca tcattaaaat cagaataaaa
     3661 aaataccacc actatggcca gcgcattaaa aaatctttct gcctacaacg aatccgacat
     3721 gccctccgcc gccgaaatgt ctttcggtat tgcggtcgcc gactggaacg cccacatcac
     3781 ccacgccctc tacgaaggct gtgtggaagc gctgctcaaa cacggcgctt tgcccgaaaa
     3841 catacacacc atgcaggtac ccggctcctt cgagttgcct gccgcctgcc gcctgctgct
     3901 cagcagcaaa aaaatggacg ccgtcattgc cctcggctgc gtcattcagg gagaaactaa
     3961 ccacaacgaa tacatcaacc acgccgtagc caacggcttg gtacaattgt cgctggccac
     4021 cggcataccc tgcattttcg gcgtactgac gcccaacaac gagcagcagg ccctcgaccg
     4081 cgcgggtggc gtacatggca acaaaggcgt agaggccgcc gcaacggcca tacgcatggc
     4141 agcattaaaa aaggaatccg gcacgccgaa aaagcgcatc gggttttgag gcaggcttat
     4201 accaaaaacg gctgccttgc ttcctgcccg atatccttca tccgggcaat ggcttcggtg
     4261 ggattgtcgg cgccgaaaac actgctgccg gcgacgagta catcagcgcc ggcctgaagt
     4321 attttttcag cattctggag gcccacgccg ccatccacct caataagcgg gtaaagattc
     4381 cgctcatcgg ccatttggcg cagccgcgct attttgggta tgctgcggta gatgaacttc
     4441 tgaccgccga aacccggatt gaccgacata atacacacca aatcaaggtc ttcgagcaca
     4501 tcgtccaaca actctaccga ggtatgcggg ttgatggcca ctccggcttt ggcgccggta
     4561 tctctgatct gttgcagggt gcggtgcaag tgcgggcaag cctcgtaatg cacggtaatg
     4621 acatcggcgc cggcagcccg aaacggctcg atgtagcgtt ccggctccac gatcatcagg
     4681 tgaacatcca aaggtttggc cgacattttg cgaatggcct gcacaatgaa catgccgaaa
     4741 gtgatattgg gaacgaagcg gccatccatg acatcaaggt gcagccagtc ggcgcggctt
     4801 tgttcaacca tagccacatc ccgctccaag cgagtgaagt cggcggccaa taaagaggga
     4861 gcgatgaggt gattgcgtat catgggggca aaaataaaaa aaccggctgg gttttccagc
     4921 cggtcgaaaa gagctttgag aggctatcta cataccaaat gcgagtatcc tttgatactc
     4981 tttagtcttt cacgagatta taatttgtta ttcgcggatc ataatgttag cagtaagtcg
     5041 tcgtcgtctt atttcgtttg acaatgcaaa gatgcagccg cccggcaccc gaaaatagta
     5101 cattnnnnnn nnnnnnnnna ctggacttga attgtgaaag tttttttaaa ttaataaatt
     5161 gattttcaat attttaaaat ttaagtaaag aaattatttt gaattagcgt tctgattttt
     5221 tgatttcttt atgacttcct gcaaatgaaa aaaaatgaaa aaatcgtttc aactccacaa
     5281 aaccagcaac atgatgaatg aatttttgcg ggcttgcctg cttttcaccg cactgcttct
     5341 gcaactctcc ttgtctgcac aatggacctg gcaggaaacc ggcaacgcct cctactatgc
     5401 cgacaaattt catggcagat caactgccag cggagaacga tacaataaaa acgaactgac
     5461 agcagcacac cgcagccttc cgatggggtc cattgtacgc gtgacaaatg ctcgaagcgg
     5521 ccaatcggta gaagtgcgca tcaacgattg cggcccgcat cgatccgatc gggtcattga
     5581 tgtgtccaaa gcagcggcca aaaaacttca cctcattgcc gacggggtag ccccggtcat
     5641 tgtagaattg gtggaaccag gcgaggggca ctgcgcctgc gatcgcaaaa aacagtggga
     5701 ccccactccc tggagagcta aagaaacggt catcaccaaa gaaacggagg tgacggccac
     5761 aacgagaccg gagcaaacgc ctcccgcgcc tcgtaaagaa aaaacacagc ccggctctgt
     5821 tgccgaaaca gccctcgaat cgccggtcgt gcaggagcca ccttccacgg gtattgtctt
     5881 gcaattaggt gctttcggta gcaaggacaa tgcgaataat tttgcacaaa aaattaacaa
     5941 acaaggattt agcgatgttt ttgtcaaaga atttccgcag ggagacaaaa ccttgtaccg
     6001 cgtttatcgg ggtgcatttt catccagaac agaggctgaa acggcacaaa aagagctgaa
     6061 aggaaaggcc ggaatagatg gcgtcataac agagttgaaa ttgtaataaa atttttcggg
     6121 cttgcattgt tggcagtttc gtcaaatggt tatacattcg tcaaaaaaca gcatttatgt
     6181 ggaacaagca aaaacttttc tcggcgatca tcgccctgtt tagcctcacg gcagtacacg
     6241 ctcaatggtc tgaaaccggt atcgccgggt attacgccga caacctgcac ggcaaaaaaa
     6301 ccgtcggtgg agagctgtac gataagtatg cgctcacggc cgcccaccac aacctgccta
     6361 ttggctcgcg catcctcatc acccgcttgg ataaccaaca gtcggttgaa gttcgcgtca
     6421 atgactgctg ccttgccgtc aaagggcgca tcgtttcgct gtcgcgcgct gccgccgaaa
     6481 aaattggttt gattcaggcc ggcacggctc aggtcagaat tgatttagtg agcagaggcg
     6541 acggcaaaac ctgcgccggc gataagcccg tagcggccta ttccaaagag gcggaaaaag
     6601 tgacgcccaa aggaggtagc gctcctgccg ttgaaaaagg tccggtagcg cctggcactt
     6661 accgcgccga cgccctcaag cctatccagt ccgggtttgg cgttcaggta ggcgcttaca
     6721 gcgacaaagc caatgccgag gcgcgcgtgg aagaaatgcg caaaaaggga ttcaaagatt
     6781 tgttggtcac ttacaaaggc gccgaccaga aaatccctta taaagtcatc atcggcccat
     6841 tcgatacagc cgcttcagcc gcagcgtaca ataagaatct cgctgccaaa cacaaggtca
     6901 aaggccatgt ggtgtctttg aaagaaggct gatcaacaat tcatcgtact aatttgtgaa
     6961 aggatttgtc gccggagttt cggggcaaat cctttgtagt ggggcaaaaa taaatgctca
     7021 ttttctttag gccaaaggat tagataagac acttaaaatg aaacgtttac ttatctaatc
     7081 ctttggcgaa aatgatcact ttattttttt atcgcccctt tcatgtttca catcatcaaa
     7141 tccttaccca tcatgctctc ggccatcgaa aaccctcaaa tccagcaaat tctcaccctt
     7201 ccttccgatt cggatgccgg ttggcgccgc gatctggaac gcctccgcaa cggcgaagtg
     7261 aatttgagcc gcaaaaacat cggcgaaaac tccatcaagg ccattcagcg cctgttcatt
     7321 tttctggggt attctactgc atcgtccggt gcattttcta ttgacggcga cttcggcagg
     7381 ggtaccaacc gggccgtggc gcagttccgt tttgaaaata atctcagcac caccatcacc
     7441 cgcgctacat tgtgctaccc ttgcacctgg caaacagcgc ggcaagccat cgtagccata
     7501 ccggatacgc tgctggatgt tcccagtttg gaaaaattgc tgtccaatgc cgtcgaagcc
     7561 attgccaacg ggcaggtcaa ttgtggcaat ttcgatgtcg ccgtcgaaca cctcaatggt
     7621 ttacaccaaa accgcctgat gacctgcaaa caaatatggg aacgatacgg cgcacaggca
     7681 aaaaccgccg ccgctcaaat tgaaacagag ttgcgcgtag ccatccgccc ggagtggata
     7741 ctctccatca tccggcagga aaccggcggc gtggtgcggc cccgtttcga gcagcatttg
     7801 ctcactaaaa tgaacgccca aatgccgaat accgacctgg cagagttgcg ctaccgcagc
     7861 atgagctttg gtttgggaca gattttgggt accaacttcc gtaccgtagg cgccgcctcc
     7921 gcgcgggcca tgttcaccag tccgctcgat gagcaattgc tatttgtggg gcgttttctg
     7981 gccaaaaacc gggaaatggc caaagtggtg gtgaaaaaac aacccgtttc agaggatttc
     8041 cacaccatcg gcaaatacta caacggcccc ggctatgcca agcaccacta ccacgaaagc
     8101 attgagcgct ggttcaaaga gtttcggatt ttgataggct gagaagaata tggcctctac
     8161 cgagactatt tcatcagagc ttgtgttgaa aggccacttg cagcgaaaaa tagtgcgggt
     8221 aataagtggc ggtggcgccg acctcgctgg tgctttttac aggcgacaaa gcatacgcat
     8281 acaaaggcat gatatggatg ctgttgcgtt cccactgggc caatgtacac tggatgccca
     8341 acagtgcaga cagattgaat ctgggcagat cgtaatgatc cagcgtaaga tcatctcctc
     8401 tgccgcttat cctcaaggtg gtaacgggcc cccattggcg ggaagcagtg aacccgacgg
     8461 caggagcaat ccggtggagc ctttgggaaa tgcttctgcg ccacatcacc gtgcatcctg
     8521 catcatacaa tttgctggtc gttttataag tagtaaattc gccattgaag ggccaatccg
     8581 aaccccacct tgtagtcagg gtttctgtaa agaccaaacg atggaggctg atgcctgcgc
     8641 aaaaagaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnccttc ggcaggtcgg gttacattca tgccggcccg
     8761 catttcgatg ccgtcaaatt gggcgtgggc gattgaaatg cttgcaaaaa cgaaaagcgc
     8821 cgccatcagt ttgcgaagca gtccgttgtt gaaataactg tttttcatgt gcgggaagtt
     8881 ttacgagtta tttatcaaca acgttcttct cgtcattttc gcgggttgag gggtggaaat
     8941 attattgtta ggttgcaacc tggggcgggt tcaataatat ataaggtaca aaagagcaaa
     9001 catggaacgt gaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nncatgtagc gcaggatcag gtacgccagc atgaccaatg
     9121 cggagagcgc agccgaaacg taggtcatgg cagcccattt gagcgcatct tttgcgccgt
     9181 cgtattcagc gccctgcgtg gtgcgcgagt tttccaacca aactaaagcg cggcggctgg
     9241 cgtcgaactc taccggcagc gtgacaacgc tgaacaaggc ggtgacggca aaaaccacaa
     9301 tggctgccag catgatttgc ggcatggtat tgatggtcac aaatgcagcg atgaggatga
     9361 attgctgcaa ggaagatgaa atctgcacca ccggcacgat ggccgagcgc acctgcaaca
     9421 tagcataagc ctcggcgtgc tgcactgcat ggccgcattc gtgggcggct acggcagcgg
     9481 cagcaatgct ccgctcgtga tagaccggct cgctgagact cacggttttg gtcatcgggt
     9541 tgtagtgatc ggtgaggtag ccctggcccg gcaccacttt cnnnnnnnnn nnnnggtgat
     9601 gttgtaatag cgcagcatgg cttcggccac ttcggcgccg ctcatgccgg agcgcagcgg
     9661 aatctggctg tatttgccga atttactttg cagcttctgc tggacgacga aacccagcac
     9721 cgacaaaacg ccggcgatga tcatgtaaaa cggatcgaaa atcatggttg tgtcaatgtt
     9781 tggttagaat cagttgatga tatcaaatcc tgtgtagggt ctgagtacag caggaataac
     9841 aatgccggct tcggtttggt tgttttccag cagggccgcc acgatgcggg ccaatgccaa
     9901 cgcgctgccg ttgagcgtgt gcaggaggcg gttttggccg ctgtcgcgga aacggagcat
     9961 catgcggttg ctctggaaag tttcaaaatt ggaaacagag ctgacctcca gccaacgctg
    10021 ctgtgctgcg gagtaaacct cgaagtcgaa agtaagggca gaggtgaagc ccatatcgcc
    10081 gccgcacagg cgcaggacgc gataagggag ttccaactcg ttgagcaggc cggaaacgtg
    10141 ctccagcatt tcgtcgaggg cttggtagga gcggtcgggg tgttccaagc gcacaatttc
    10201 tattttgtcg aactggtgaa cgcgattgag gccgcgaaca tgggcgccgt aagagcctgc
    10261 ctcgcggcgg aagcaaggcg tgtagcccgt cattttgacc ggcagatcgc tctcattgag
    10321 aatcacgtcg cggtagatgt tggttaccgg cacctcggcg gtaggaatga ggtagaggtc
    10381 gtcgcgctcc acataataca tttgcgcttc tttgtcgggc aattggccgg tggcgcgggc
    10441 cgtatcggca ttgaccatca gcggcggggc aatttcttcg tagccggcag tcgtggcgcg
    10501 gtcgaggaag aggttgatga gcgcgcgttc gagccgggcg cctttgccgc gataaacggg
    10561 gaagcctgcg ccggtgagtt tgacgccgag ttcgaggtcg aaaaggttgt attttttggc
    10621 caattcccag tgcggcaggg ccgattcggg cagtaccggc agttcctgcg gccaggcttt
    10681 gaacacctcg ttgtcatcgg ctgttttgcc ttccggcaca gatgcgtgcg gaatgttggg
    10741 cacctgatag aggagttctt cgagttgatt tttggtgtcg gccagcgtga tgcccaattg
    10801 gtctgctttt tctttggcgg cagccacctg cgctttgagg gcttcggctt cgtcgcggcg
    10861 gccctgtttc atcaagtcgc cgatttcttt ggaaagagcg ttgcgctggg cgagcagggc
    10921 atcggattcg gactggatat ttttgcgatt gtcgtcgagt tcgaggatgt tgtccacgat
    10981 gcgaagttgg tcgtcagaga agttgcgttt ttgcaagcct ttgataacga cctctttgtt
    11041 ttgccggatg aaattgagtt gcagcataag tgattcttga cacgcggctt tcccgcgggg
    11101 gatgttgaaa acttttgtta tttttgtgag gcaaagatac tacttggttg gaaaagtagt
    11161 tatctttgtc gcagcgtaag tggccaatca tcgccaaaat cggtacctcc gcgctcattt
    11221 atcacctttt ttatcacctt atttttgcac ctctgcttcc agccgaaggt cgtgcgcctt
    11281 gctcgaaggc ttcttcacaa gttcccttgc tggataattt taattactta caccacatgt
    11341 acgaaatttc gcaattgagc gatatgctcg ttcccgaatt gcgggaaatt gctgatggtc
    11401 tgaacattaa aggctatcgc aaactgaata aacctgatct tattcaaagt atcctggatc
    11461 atcagacgcc cgccgttgcg gaagttgtcg cagaaccgcc ggcgccggaa cccgaaaagc
    11521 cgaaaagagc catgccgtct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnacgatgac gacgacgatt ttgccccccc gcctcatgct
    11641 gccattttgc ccgctttcaa cccggaagat gtttatctgc gccgaagcca tagttacacc
    11701 cgtgaattgg aaggtaaact ttccgactcc ggggatgaca acccggaagc ccgccgccgc
    11761 cgtgcgcgca aagtagtaga gcgtggcccc gatcgccccc agccccccat gcccgaccgt
    11821 tcgtcccgcc atgaaatgcc caaatatcgc cagcctgagc ctgaattgga acccgaagaa
    11881 gaggaatttg aggagtatgt agaattgaaa ggccaacaca aaaaacctca acaaccgccc
    11941 agacctgtac cagcacctgc gcctgccccc gaaccggaaa ctccgaaaat ggtgcagccc
    12001 atacaaaccg gacccaaatt cgacatagaa ttagacggcg ttgtagaagg cttgggcgtg
    12061 ttggaaatga tgtccgacgg gtatggcttc ctgcgttctt ccgattataa ctacctttct
    12121 tcccccgacg atatttatgt gtctccatcg caggtgaagc tgttcggctt gcgtaccggc
    12181 gacaccgtgc ggggcgccat tcggccgccc aaagaaggtg aaaagtattt cgctttgttg
    12241 cgtgtttcca aaatcaacgg cctcgatccg gtggatgtac gcgagcgagt gccttttgat
    12301 tacctcacgc ctttgtttcc caacgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtagc
    12481 caatgccatt gccaaaaacc acccggagtg cttcctcatt gttttgctgg tggacgaacg
    12541 cccggaagag gtgacggaca tgaagcgcag tgtgaatgca gaggtcgtgg cttctacttt
    12601 tgacgaaccc gccgacaacc atgtgcgcgt ggccggcgtg gtgctcgaaa aggccaagcg
    12661 tttggtagaa tgtggccacg atgtggttat tctgctcgat tccatc
//
LOCUS       DAORLF010000075        12684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2641_length_12684_cov_2.214427, whole genome shotgun sequence.
ACCESSION   DAORLF010000075 DAORLF010000000
VERSION     DAORLF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_12684_cov_2.214427"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..916
                     /locus_tag="PK198_08280"
     CDS             158..916
                     /locus_tag="PK198_08280"
                     /inference="COORDINATES: protein motif:HMM:NF020448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HRF38772.1"
                     /translation="MTTYHILNGDCLADQLRQTKINQDFIVCRECLIDGNLSADNIPD
                     FWIIRANFIADTYNVSSKEYFSKTVREFEKLNNLPNNSEVCLWFENDLFCQTNMWFVI
                     SMLANHPTLKIFRVFPTIENIADTWKGFGIATAEKLEQAYHSKIQFSSQDIELGKNLW
                     TAYQDGDFDKLKKLSKNQSDCFEYLKEVCQAHIDRFPVDKTLGRPDKVVKEIIDTNST
                     EFEVVFSEFSDREGIYGFGDLQIKNIYDRLMHNH"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1074..1589
                     /locus_tag="PK198_08285"
     CDS             1074..1589
                     /locus_tag="PK198_08285"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF38773.1"
                     /translation="MKHDKMYSIFICIGMFWIFTVPVFSQDAAMDAQLLFDQLKERDS
                     LIFKLGFNNCDTAQLRKLLSDDFEFYHDQNGLMESKELFIQNIPNLCKMNYKPIRVLV
                     DSSLQVFPLFSSGKIYGAIQTGVHEFYGEEKDKPRYLTSTARFTHVWIMEGEDWKLKR
                     ILSYDHIIPKK"
     gene            1789..>2777
                     /locus_tag="PK198_08290"
     CDS             1789..>2777
                     /locus_tag="PK198_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38774.1"
                     /translation="MSSTIKNTLIAIGIPTIYAVVLRLFFGIATWYDLFSVMSFTFLF
                     VLPIIVGALTVYLSNPEKARRIAYRIFAPWIPILLFLVITLALAIEGWACWLMILPVF
                     LIAASIGGLIGGYLKTRKAHNRLNISLLVLLPFFIGPIESLIETIPGTYKAYTYIDID
                     APAEKIWDNVTRVKEIPIEQDKGYLTRILGIPRPVKAELDFEGVGAYREAVFTKGLVF
                     HETVTEYKDNEKMVFNIKAYPHEIPATTLDEHVLIGGDYFDVLNGTYELEKLPNGLNR
                     LHLYSHFKMNTTFNFYAGWWGKWIMKDIQNNILQIEKIRSEEKSSPATMSPVH"
     assembly_gap    2778..2800
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2815..>3252
                     /locus_tag="PK198_08295"
     CDS             2815..>3252
                     /locus_tag="PK198_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38775.1"
                     /translation="MTITDIENLCFVSDTIKKDFSSLIGTVESCENDLPAMTCMNFSD
                     GSKNLFELDDSTVIIPFRLTEYDLEMMIFTKWIKKTDTTFTFLGRLYPNMLSKYASYE
                     ILERAKINNAEYLIGAIYGGEGGKMSQRIWTAKFDGITLTDFVN"
     assembly_gap    3253..3281
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3782..4576
                     /locus_tag="PK198_08300"
                     /pseudo
     CDS             3782..4576
                     /locus_tag="PK198_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
     gene            4573..5055
                     /gene="paaJ"
                     /locus_tag="PK198_08305"
     CDS             4573..5055
                     /gene="paaJ"
                     /locus_tag="PK198_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRF38776.1"
                     /translation="MKTEDTIWAVLESVTDPEIPVLSVTDMGIIREVHAGEEVEVVIT
                     PTYSGCPAMNTIEVNIRAALQDAGYERVRVRTVLSPAWTTDWLTEAGRRKLKEYGIAP
                     PQDAVADKNALFGTPRVVPCPQCDSENTELMSQFGSTACKALYRCKDCLEPFDYFKCH
                     "
     gene            5069..5776
                     /locus_tag="PK198_08310"
     CDS             5069..5776
                     /locus_tag="PK198_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRF38777.1"
                     /translation="MTTKLSLLRWLHDFSGLFYPRLCLACAERQPPQNSLICQTCQAG
                     LPRTDLHLHQDNAFTQRFWGRVPVYSGGAMFHFVKGGKTQKLLHRLKYEGKPQIGVEL
                     GKWYGRELKQSPWFADVQAIVPVPLHPRKERLRGYNQSDTIARGLSEGMKTPWYKEGL
                     RRREFTETQTQKSRMERFGNVEEVFEVGNPAALAGKHILLVDDVMTTGATLEACARVL
                     LTLPDLRISMATLAMAT"
     gene            5825..7126
                     /locus_tag="PK198_08315"
     CDS             5825..7126
                     /locus_tag="PK198_08315"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRF38778.1"
                     /translation="MKKTLKVFKFGGASIKDADSIKNVASILERFREEKLIIIVSAMG
                     KTTNALEKVVSAHNYGNTELARQLLDDLKKAHYQIMDALFEPGDEVYSIVNDTFVESE
                     WVLDEPPHENYDFMYDQIICVGELAASRIVSSYLNRRGLRTQWLDARDVILTDDIYRE
                     GWVQWEETEERYAHKVPPMLESPGFVLTQGFIATTSENATTTLGREGSDYTAAIFSHL
                     SDAESMTIWKDVPGVLTADPRIFDNVSKLDRLSYKEAIEMTYYGATVIHPKTIKPLQN
                     KSIPLYVKSFVNPDGEGTVISGDVEEVYPPIVTIERNQALLQISTRDFSFVAEHHISQ
                     LFTLIADQRLQVNMMQNSAISFSVCVNDTDDKVLRFIGHIEKNFKVILDRELQLITVR
                     HYTEEVLQNLLGGKIVMMEERSRGTVQVVVKDVPVISWKAS"
     gene            7159..8121
                     /locus_tag="PK198_08320"
     CDS             7159..8121
                     /locus_tag="PK198_08320"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38779.1"
                     /translation="MVQTLDIGACVASLLYDNNSVSLPGLGSITAQYQAAAIDHVQGR
                     ISSPGKALTFDTNLVLDDGLLAQTAAQRFGVSLHIATEAVSDYVRSIQNTLDRREMFA
                     LPGVGRLYRDFEQKIQFISDEHNFNPESYGLPSVQYYPVVRRAAQEAAAATPAPTRAV
                     ASWWKRNLVWLSAAAMLVLAAGIYWAVSGGKPSDPTQSTADIPEEMHNVPPGGVPSEP
                     AEEPETTPPAAAEEEIADESESPTPSPDQRVCTIRLGRFGNADNVKRLVKKAQQLGLS
                     PYTEKVGNLTEVGVTFEYSEESEIKETLRLARRSLAKDAVVESQ"
     gene            8135..8974
                     /locus_tag="PK198_08325"
     CDS             8135..8974
                     /locus_tag="PK198_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF38780.1"
                     /translation="MSKPIKIGLCLSGGGAKGIAHIGVLKAFRETGIFPDVVAGTSAG
                     SVVGALYAAGKTPEEMIDFVRDGSLFKIYKVIMPSDGLTKLTYLKDKLRGAIPEDSFE
                     GLARPLHVAVTNLITGNVEIKNSGPLFDVIMASSSIPLVFSPVEIDGQIYVDGGMLAN
                     FPVQPLIGQSDVIIGVNLMPQEPISEKSVQNIIGIATRCFSLSVWSNTQPQLRHCDLV
                     ISPPNIEQYNIFQFSRYKELVELGYEAALDRIPELLEIIDTARKEKKKAAEKKKGKKG
                     KGK"
     gene            complement(8975..9694)
                     /gene="dapB"
                     /locus_tag="PK198_08330"
     CDS             complement(8975..9694)
                     /gene="dapB"
                     /locus_tag="PK198_08330"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRF38781.1"
                     /translation="MLGYGKMGRAIERIATEAGHEIVLRIQNSSQWQENEAVIRRADV
                     AIEFSRPEAAFQNVMNCIAAGIPVVCGTTGWAERLPEARQFCMERQGAFFYASNFSIG
                     VYIFMETNRRLAQLMGRRPQYETDIEEIHHVHKLDAPSGTAVTLAETVLPYLEHKKDW
                     ALENPQSPAPETLPIRSIRSGEVPGTHIITYRSAIDTIVLIHEAHSREGFAAGAVEAA
                     QWIVGKHGCFGMEDMMQAEAI"
     gene            complement(9926..10378)
                     /locus_tag="PK198_08335"
     CDS             complement(9926..10378)
                     /locus_tag="PK198_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38782.1"
                     /translation="MKKAILFFAFVLGVSFVAQAQCSGSKTETGTAAASCCSKAKAST
                     ASADAVKAVGSDVEQRTNAAGEVSYVRKMVNAETGAVSYKAVEYCTKSNQFVDAAAAS
                     GAAPAAAAGSASGGSKASCSSGEAKAGCCSKAGAAEAPASKKGKKAKA"
     gene            10503..10976
                     /gene="coaD"
                     /locus_tag="PK198_08340"
     CDS             10503..10976
                     /gene="coaD"
                     /locus_tag="PK198_08340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRF38783.1"
                     /translation="MPKIAVFPGSFDPITTGHVEIVRRALPLFDEVVVAIGVNSQKKY
                     LFSLEQRLQWLRDVFAGEPKVRVDFFEGLTAHYCMRIGARFLVRGLRNASDFDYEKTI
                     SQLNDIVGHGVDTIFLISRPNFSHISSTIVREIIRGGGDAGPFLPPEVVVEHGAD"
     gene            complement(10973..>11200)
                     /locus_tag="PK198_08345"
     CDS             complement(10973..>11200)
                     /locus_tag="PK198_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38784.1"
                     /translation="HCEVPVLRSNISSMPEVAGEAGWPVNPHSEQSIAEGLLRLWSDD
                     SLRRQLTGQAAAQRAKFSWERAADVVAGVLR"
     assembly_gap    11201..11210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11211..>11736)
                     /locus_tag="PK198_08350"
     CDS             complement(<11211..>11736)
                     /locus_tag="PK198_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38785.1"
                     /translation="VRRYYQYFVPKFLERAERIVAVSEYTAHDIELQYGIPRPKISVA
                     CNGCDPAFRPLSEAEKQRVREQYARGLPYFFYIGAMHPRKNIPRLIAAFDQFKTNSGS
                     PALLLLAGRMAWQTGEIRSAFDAAAHRNDIIFLGYVPDEALPSLTGAALAAFGMLRAQ
                     RRLLRRLGPLTPGNG"
     assembly_gap    11737..11820
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11821..12113)
                     /locus_tag="PK198_08355"
     CDS             complement(<11821..12113)
                     /locus_tag="PK198_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF38786.1"
                     /translation="MRIAVNARFLLPGRMEGIGRFTWEVARRLVDRFPDDEFIFLFDR
                     PFDSAWLPSGNVKPVVAAPPARHPLLWYAWFEWAVPMALQRHRADVFFSPDGD"
     gene            complement(12113..12469)
                     /locus_tag="PK198_08360"
     CDS             complement(12113..12469)
                     /locus_tag="PK198_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRF38787.1"
                     /translation="MKNLLFLALLFIAAGAAQAQTDQFTVYVEGLTCPICSGGLETKF
                     KAVKGIAQVKADFKTGKMTFTMPPRYKMQMSEIKERVAKAGYAAKGILVRRSDGKTEK
                     WGELKSIAPPVTGPGS"
BASE COUNT         3249 a         3173 c         3187 g         2919 t
ORIGIN      
        1 tagccgccga ctggtcttct tcgatgcgca gccgaggtat gcaccattta tggtcacaca
       61 gggcggcgaa aagccaaacc gcaaagccgg cgctcggcgg cacggcagca agccaagcgt
      121 tattgacaat gcgacaaccg acaacaaaga caaataaatg acaacttatc acatactaaa
      181 cggagactgt ttggcggacc aattaagaca aacaaaaatt aatcaggact ttattgtttg
      241 tagagaatgc ttaattgacg gtaatttatc cgctgacaat atccctgact tctggataat
      301 ccgagcaaat tttattgctg acacttacaa tgtatcgagc aaagaatatt ttagcaaaac
      361 tgtacgtgaa tttgaaaaac taaacaatct acctaacaat tccgaagttt gtctttggtt
      421 tgaaaacgat ttgttttgcc agacaaatat gtggtttgta atctcaatgc ttgcaaatca
      481 cccgacactt aaaatcttta gagtttttcc gacaattgag aatattgcag acacttggaa
      541 aggctttgga attgctactg ctgaaaaact cgaacaggct tatcattcaa aaattcaatt
      601 ttcatcccaa gacattgagt taggaaaaaa tttatggaca gcatatcaag atggcgactt
      661 cgataaattg aaaaaattat ctaaaaatca atctgactgc tttgaatatc taaaagaagt
      721 ttgtcaagcc cacattgaca gatttcccgt agacaaaact ttgggcagac ctgacaaagt
      781 agtaaaagaa ataattgaca caaactcgac agaatttgaa gtggtttttt cggagttttc
      841 ggacagagaa ggaatttacg gatttggaga tttgcaaata aaaaatatat acgacagact
      901 gatgcacaac cactaacacg ggttttgcgt cagacggggt gacgtgcctc tattgacaat
      961 tttgtgtnnn nnnnnnnaat tagaagtgcg gctcttcgta tcaagtctat tgctaaaaag
     1021 cccgcccgaa cgccaagcca aaaaccgttg actctcatca aaaacgcaac aaaatgaaac
     1081 atgataaaat gtacagcatt tttatttgca tagggatgtt ctggattttt acagtacccg
     1141 ttttcagcca ggatgcagca atggatgctc agttattatt tgatcaatta aaagaaagag
     1201 atagtttaat ttttaaactt ggatttaata attgcgacac cgcacaattg agaaaattgt
     1261 tgagtgatga ctttgaattt taccatgacc agaacggtct tatggagtca aaagaacttt
     1321 tcatccagaa tatccctaac ctatgcaaaa tgaattacaa acccatcagg gtgttggtgg
     1381 atagcagcct tcaggttttt cctttgtttt caagtggaaa gatatatggc gccattcaga
     1441 ccggcgtaca tgaattctat ggagaagaaa aggataagcc gagatacctg accagtacag
     1501 cgagattcac tcatgtctgg ataatggaag gggaggattg gaaattgaaa agaatactga
     1561 gttatgacca cataataccg aaaaaataaa aaagacgaaa agcgaatcaa gtagttggaa
     1621 gcgaccagaa tttttgaatt ttgcccccta agaccgcaac aataagggat aaaaatggat
     1681 tatgtatctt tccttcccga caaaacgaac actccggcaa gagcatacac aatatgtatg
     1741 tgtcgtttgc ttacgtccaa acaaactttg accaataaat tgaaaaaaat gagcagtacg
     1801 attaaaaaca ccttgattgc gattggaatt ccgacaatat acgcggtagt ccttcggctt
     1861 tttttcggaa tcgctacctg gtacgactta ttctcggtca tgtcctttac ttttttgttt
     1921 gtgctgccga taatcgtcgg agcgctaact gtttatttgt caaaccctga aaaagcaagg
     1981 cgtatagcgt atagaatttt tgccccgtgg atacctattt tgctgttttt agtaataaca
     2041 cttgctctgg caattgaagg atgggcttgt tggcttatga tattgcctgt gtttttaata
     2101 gccgcctcca tcggcggact gattggcggt tacctgaaaa ctcgaaaagc tcataacagg
     2161 ctcaatattt cacttttagt tcttttgcct ttttttattg gtccgattga aagtttaatt
     2221 gaaaccattc ccggcaccta caaagcatac acctacatag atattgatgc cccggctgaa
     2281 aaaatctggg ataatgtaac tcgcgtaaag gaaattccta ttgaacagga caaaggatat
     2341 ttaacaagaa tattgggcat tccccgcccg gtaaaggcag aattggattt tgagggagtc
     2401 ggcgcataca gagaggctgt ttttacgaaa gggcttgttt ttcacgaaac cgttacggag
     2461 tacaaagaca atgaaaaaat ggtcttcaat attaaagctt atccccatga aataccggca
     2521 actacattag acgaacatgt gttaattggc ggagactatt ttgacgtact caatggaact
     2581 tatgaacttg aaaaattgcc aaatgggctg aatcgcctgc atttatacag ccattttaaa
     2641 atgaatacga cgttcaattt ttatgccggc tggtggggaa aatggatcat gaaggacatc
     2701 caaaacaata tcttgcaaat tgagaaaata aggtcggaag aaaaatcaag tcctgccacc
     2761 atgtcgccgg tgcatatnnn nnnnnnnnnn nnnnnnnnnn cattaaaatc ggaaatgacc
     2821 atcacggata ttgagaatct ttgttttgtg tcggatacaa taaaaaaaga tttcagcagc
     2881 cttattggca cggttgaaag ctgcgaaaac gacttgcctg caatgacgtg tatgaacttt
     2941 agcgacggaa gcaagaactt gtttgaactt gatgattcga ccgttattat tccgttccgg
     3001 ttaacggaat atgaccttga aatgatgatt tttactaagt ggataaaaaa gacagatact
     3061 acctttactt ttttaggacg gctttatccg aatatgcttt ccaagtacgc ttcttatgag
     3121 atattggagc gggccaagat caataacgca gagtatttaa tcggggcgat ttatggtgga
     3181 gaaggcggaa aaatgagtca acgtatctgg acagcaaaat ttgatggcat caccttgacc
     3241 gactttgtca atnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatggcgag gagcatagaa
     3301 aaacacttag ttataaacgg aaaaaaaata ttctttcgat aagaatgcac acagattcaa
     3361 ccgtatatga gagaggcata gaaacgtata ttcctcttgg tagtaaaatt gtaaaaacgg
     3421 ttgagttaaa atgactactt ttgagacggt tccgatactc ttgaaatcgg catcctcgcc
     3481 ggattggcca accacaaaat caacttttta taacaccatg tggagagact tcaaatacct
     3541 catcgcctac atcctccccg gctcggcctt tgtggcgctg tatgcccagg gcgcgtggtc
     3601 gttcagtacg gtggtagttg cctttgtgtg cattccagtg ctggagcaat tgctgcctca
     3661 gtcggtggcc aattggagtg aagaagagga gtcccggcag tcggcaagaa catttttcga
     3721 cctgctgctg tacctcaacg tacccattct gtacgccatg ctgtacgact atttcaattt
     3781 gatggcgcag ggcggtctgg caacgtatga gcttgtcggc ttgacgctca gcgtcggcat
     3841 cacggtggga gccatcggca tcaatgtggg ccacgaactg gggcaccgcg cccggcgagg
     3901 cgaccaattg ttggcgcaag ccctcctgct gccggccttg taccagcatt tcaccataga
     3961 gcacaaccgg gggcaccaca aaaacgtagc caccgatctg gaccccgcct cggcccgccg
     4021 gggacaaacc acttatgcct tttggtggca atccgtgacg ggcggctacg ggaatgcctg
     4081 ggcgctggag cgggagcggc tggagcgcgc cgggcggccg gttttgtagt ggaacaacca
     4141 gatgatccgc ttcacgctca tgcaggcgct ctggttggca gccttgggcc ggcttttcgg
     4201 atggaatatt ttgccctggg ccatagcggt ggccgtcata ggttttttgc tgctggaaac
     4261 cgtcaattac attgaacatt acgggctgcg gcggcgcttg ttgccatcgg gcatatacga
     4321 aatcgtgcag ccgcatcact cctggaactc cgatcacgaa ctgggccgca tcatgttgta
     4381 cgaactcacg cggcattccg accaccacta caaagccaat cgcaagtatc agatactgag
     4441 gcattttgac caaagtccgc aattgccttt cggctatccg gcctctattt tgctggcgct
     4501 ggcgccgccc ctgtggttcc ggctgatgca cccgctgttg gaccggctcg aatctcaaaa
     4561 cactgcacaa gtatgaaaac cgaggataca atatgggccg tattagagtc tgtgaccgac
     4621 cctgaaatcc cggtgctgtc cgtcaccgat atgggcatca ttcgggaggt acatgccggc
     4681 gaggaggtag aagtcgtcat tacgcccacc tattcgggct gcccggccat gaacaccatt
     4741 gaagtgaaca tcagggcggc attgcaggat gccggttacg agcgggtgcg cgtccgaacc
     4801 gtgctcagtc cggcctggac gaccgactgg ctcaccgagg cgggccgccg caagctgaaa
     4861 gagtacggaa ttgcgcctcc gcaagatgcc gtagccgata aaaatgcgct gttcggaacg
     4921 ccgcgcgtgg taccctgtcc gcagtgcgat tctgaaaata cggagctgat gagccagttt
     4981 ggttccacgg cctgcaaggc gctctatcgc tgcaaggatt gcttagagcc gtttgactat
     5041 ttcaaatgcc attgaaagag tgtgtattat gaccacaaaa ctctccctcc tccgctggct
     5101 ccacgatttt tcgggcttat tttatccccg gctttgtctg gcctgcgccg agcggcaacc
     5161 acctcaaaac agcctgatct gccagacctg tcaggcaggt ttgccgcgca ccgatctgca
     5221 cctgcatcag gataacgcct tcacccagcg cttttggggc cgcgtgcccg tttacagcgg
     5281 cggcgccatg tttcattttg taaaaggcgg caaaacgcaa aaactcctcc accggctgaa
     5341 gtacgaggga aagcctcaaa tcggcgtgga attgggcaaa tggtacgggc gggaactgaa
     5401 acagtcgccc tggtttgccg acgtgcaagc catcgtcccc gtgccgctgc acccgcgcaa
     5461 agagcgattg cggggctaca accaaagcga caccatagcc cgcgggctgt cggagggcat
     5521 gaagacgccc tggtacaaag agggtctgcg cagacgggaa tttacggaaa ctcagaccca
     5581 aaaaagccgc atggagcgct tcggcaatgt ggaggaagtg tttgaggtgg ggaatccggc
     5641 cgcgctcgcc ggcaaacaca ttttattggt ggacgacgtg atgactaccg gcgccaccct
     5701 cgaagcctgc gcccgcgtgc tgctcaccct gcccgacctg aggatcagca tggccaccct
     5761 tgctatggcg acgtaaaatt ccttaccttt gcgccctgat tcacaacaag tacggtttta
     5821 ccaaatgaaa aaaacgctca aagttttcaa attcggcggg gcctccatca aagacgccga
     5881 ttccattaaa aatgtggcct ccattctgga gcgattccgg gaggaaaaac tcatcatcat
     5941 cgtttcggcg atgggcaaaa ccaccaacgc attggagaag gtggtgagcg cccataatta
     6001 tggcaatacg gaactggccc gccaactgct cgacgatctc aaaaaggcgc attatcagat
     6061 catggatgcc ctgtttgaac ccggcgatga agtgtattcc attgtaaatg acacctttgt
     6121 agaaagcgag tgggtattgg acgagccgcc gcacgagaac tacgacttca tgtacgacca
     6181 gatcatctgc gtgggcgaac tggctgcctc ccgcatcgtc tcgtcatacc tcaatcgccg
     6241 cggactgcgc acccaatggc ttgatgcccg cgatgtcatt ctcaccgacg atatttaccg
     6301 cgaaggctgg gtgcaatggg aagaaaccga ggaacgctac gcccacaaag tgccgcccat
     6361 gctggaatcg ccgggctttg tactcacgca gggcttcatt gccaccacca gcgaaaatgc
     6421 caccactacg ctgggccggg aaggctccga ctacacggcg gccatttttt cgcacctcag
     6481 cgatgcggaa agcatgacga tatggaaaga cgtacccggc gtattgactg ccgatccgcg
     6541 catcttcgac aacgtgtcga aactcgaccg gctttcttac aaagaagcca ttgaaatgac
     6601 ctattacggc gccacggtca tccacccgaa aaccatcaaa ccgctccaaa acaaaagcat
     6661 accgctgtat gtaaaatcat tcgtcaatcc cgacggcgag ggcacggtta tttcgggcga
     6721 cgtagaggag gtctatccgc ccatcgtgac gatagagcgc aatcaggcat tgctgcaaat
     6781 ttctacccgc gacttctctt tcgtggccga acaccatatc agccagttgt ttacgctcat
     6841 cgccgatcag cgcctgcaag tgaacatgat gcaaaattcg gccatcagtt tcagcgtttg
     6901 tgtgaatgat accgacgata aagtgctgcg tttcatcggc catatcgaga aaaatttcaa
     6961 agtcatcctc gaccgcgaac tgcaactcat cacggtgcgc cactatacag aagaggtgct
     7021 ccaaaatctc ctcggcggca aaattgtgat gatggaagaa cgcagccgcg gcacggtgca
     7081 ggtggtggtg aaagacgtgc cggtcatcag ttggaaggca agttgatggt tttccttcag
     7141 ttttaataca tttgcaacat ggtacaaacc ttagacatcg gcgcctgtgt ggcatctttg
     7201 ctgtacgaca acaacagtgt gagcctgccc ggcctgggaa gcattaccgc tcaataccag
     7261 gcggctgcca ttgaccatgt gcagggcaga atatcatcgc ccggcaaagc gctgactttc
     7321 gataccaatc tggtactcga cgatggcctg ctggcccaaa ctgccgccca acgtttcggc
     7381 gtatcgctgc acattgctac ggaagccgtg agcgattatg tgcgcagcat acaaaacacc
     7441 ctcgaccgcc gcgagatgtt tgcactgccc ggcgtagggc gcctgtaccg cgattttgaa
     7501 caaaaaatcc agttcatctc cgacgagcac aatttcaatc ccgaatcgta tggactgcct
     7561 tctgtgcagt actacccggt cgtgcgccgc gctgctcagg aggctgctgc tgcaacacct
     7621 gctcctaccc gtgccgttgc aagctggtgg aagcggaatt tagtttggtt gagcgctgcg
     7681 gccatgctgg tgctggctgc cggcatttat tgggcggtgt cgggcggcaa gccatccgat
     7741 ccaacgcaaa gcacggccga tattcccgaa gagatgcaca atgtacctcc cggcggggtt
     7801 ccatctgagc ctgctgaaga acctgagacg actccgcctg ctgccgcaga agaggaaata
     7861 gcggatgaat ccgaatcgcc tacgccgtcg cccgaccaaa gggtttgcac catacgtctg
     7921 ggccgttttg gcaatgccga caacgtgaaa cgcttggtaa aaaaagcaca gcaactcgga
     7981 ctcagtcctt acaccgaaaa agtcggcaac cttacggaag tgggcgtcac cttcgagtat
     8041 tcagaagaaa gcgaaatcaa ggaaaccctg cggctggccc gcagatcgct ggccaaagac
     8101 gccgtggtgg aaagccaata aacgcagaca tttcatgtcc aaacccatca aaatcggcct
     8161 ctgcctttcg ggcggcggcg ccaagggcat cgcgcacatc ggcgtactca aggcttttcg
     8221 cgaaacaggc attttccccg atgtggtggc aggcaccagc gccggctcgg tggtaggcgc
     8281 cttgtatgcg gccggcaaaa ccccggaaga gatgattgat tttgtaagag acggcagcct
     8341 gttcaagatt tacaaagtca tcatgcccag cgacggcctc acaaagctga cctacctcaa
     8401 ggacaaactg cgaggcgcca ttccagaaga cagtttcgag ggcttggcgc gcccgctgca
     8461 cgtcgccgtc accaatctca tcaccggcaa tgtggagatc aaaaactccg gcccgctgtt
     8521 cgacgtcatc atggcatctt cttccattcc gttggttttc agcccggtag aaattgatgg
     8581 ccaaatttat gtggacggcg gcatgttggc caattttccc gtgcagccgc ttatcggcca
     8641 atcggatgtc atcataggcg tcaatctgat gccgcaggaa ccgatttccg aaaaatccgt
     8701 acaaaatatc attggcattg ccacgcgctg tttttccctc tcggtatggt ccaataccca
     8761 gccccaactc aggcactgcg acttggtgat ctcgccgccc aacatagagc agtacaatat
     8821 ttttcagttc agccgataca aagaactggt tgagttgggt tacgaagctg ccttggaccg
     8881 gataccggaa ctgttggaaa tcatagacac ggcccgcaaa gagaagaaga aggcggcaga
     8941 aaagaaaaaa gggaagaaag gaaaaggaaa gtaatcaaat ggcctccgcc tgcatcatat
     9001 cctccatacc gaagcatcca tgtttgccca ctatccactg cgcggcttct actgcgcccg
     9061 cggcaaagcc ctcgcgcgag tgcgcttcat ggatcaaaac aatggtatca atggccgatc
     9121 ggtaggtgat gatgtgcgtg ccgggaacct cgccgctgcg aatggaacga atgggcagcg
     9181 tttcgggggc cggcgactga gggttttcca aagcccagtc ttttttgtgt tccaaatagg
     9241 ggagtacggt ttctgccagg gtcacggcgg taccgctggg ggcgtctaat ttgtgaacat
     9301 gatgtatttc ttcaatgtcc gtctcgtatt gcgggcggcg tcccatcagt tgggccagcc
     9361 tgcgattggt ttccataaaa atatacacgc cgatgctgaa attactggcg taaaagaacg
     9421 cgccctgccg ctccatacag aactgccggg cttcgggcag gcgctcggcc caaccggtag
     9481 tgccgcacac aaccggaata ccggcggcga tgcaattcat tacattttgg aaagctgctt
     9541 cggggcggct gaactctatc gccacatcgg cccggcggat aacagcctcg ttttcttgcc
     9601 attgagagct gttttgaatc cgcaacacga tctcgtgccc ggcttctgtg gcaatccgtt
     9661 caatggcgcg gcccattttt ccgtagccca gcaaaattat ttttaaaaag cgttgtgact
     9721 tcatcatcgt acctccgtca gaatggcatg ttttaagtaa gggagaacaa ataaacacat
     9781 ttcgcaagac ctttcgatgg cgtaatcatc gaaaggtttt tttgttttag agaatggtga
     9841 agatgtggcc actactcata cagaaacaac acaggccacc ggaacgaatc ccgatggcct
     9901 gcggtatgcc tgaaaaaatt tcagattaag ccttggcttt tttgcctttt ttggaagccg
     9961 gagcttcggc agcgcctgct ttagagcagc agcctgcttt ggcctcgcct gaagagcagc
    10021 ttgctttaga gccgccggaa gcactgcctg cagcagcagc cggagcagcg ccggaagcgg
    10081 cggcagcatc aacaaattga ttagatttgg tgcaatattc aacagccttg tagcttactg
    10141 cgcccgtttc ggcattgacc attttgcgaa cataagaaac ctcgccggca gcattggtgc
    10201 gctgttcaac gtcggaacct actgctttta cagcatcggc agaagcggtg gaagctttgg
    10261 ctttggagca gcaagaagcg gcggcagtac ccgtttcggt cttagagccg ctgcactggg
    10321 cctgggctac gaaagatacg cccaagacga acgcaaagaa caagatcgct tttttcataa
    10381 tctgattttt gtttggtgat aaaaattgtg ctgcaaaatt acggactttc caagtataat
    10441 tgtgttactg tttttttaac attttcttaa aattgccgcc gcatccaaaa tttgaccaaa
    10501 ttatgccaaa aattgccgtt tttcctggct ctttcgaccc catcaccacc ggccatgtgg
    10561 aaattgttcg ccgggcgctg cctttgtttg acgaggtggt ggtggccatc ggcgtcaatt
    10621 ctcagaaaaa atacctgttc agtctcgaac agcgattgca atggctccgg gatgtttttg
    10681 ccggcgagcc taaagtgcgc gtggactttt ttgaggggct gacggcgcac tactgtatgc
    10741 gcatcggggc gcgttttctt gtacgagggc tgcgcaatgc ctccgatttt gactacgaaa
    10801 agaccatttc ccaactcaac gacattgtag ggcacggcgt ggatacgatc tttctcatca
    10861 gccgccccaa tttttctcac atcagctcca ccatcgtccg ggaaatcatt cgcggcggcg
    10921 gcgatgccgg gccgtttttg ccgccggagg tggtggtaga gcacggggct gattagcgca
    10981 ataccccggc cactacatcc gcagcgcgtt cccaggaaaa tttcgcgcgt tgcgccgcgg
    11041 cctgccctgt caattgccgg cgcagggagt catcgctcca caagcgcaac aagccttccg
    11101 caatagactg ctctgaatgg ggattgaccg gccacccggc ctcgccggct acctccggca
    11161 tggatgaaat gttggagcgc aataccggca cttcgcagtg nnnnnnnnnn agccgttacc
    11221 gggtgtcagc ggcccgaggc gacgcagcag gcggcgctgc gcgcggagca ttccgaacgc
    11281 ggccagcgcc gcgccggtga gcgaggggag cgcctcgtcg ggcacatagc ccaagaaaat
    11341 aatgtcgttg cgatgtgccg ccgcatcgaa agcgctgcgt atttcgcctg tttgccaggc
    11401 cattcgcccg gccagcaaga gcagcgccgg cgagccgctg ttggttttga attgatcaaa
    11461 agcggcgatg agtcggggta tgtttttgcg ggggtgcatg gcgccgatgt agaaaaaata
    11521 aggcaatccg cgggcgtatt gctcccgaac gcgctgtttt tctgcttccg aaagcggcct
    11581 gaacgcaggg tcgcagccat tgcaggcaac cgatattttg gggcggggaa tcccgtactg
    11641 aagctcaatg tcgtgggccg tatattccga aacggcaacg atccgttcgg cgcgctccaa
    11701 aaacttagga acgaaatatt ggtaataccg ccgcacnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 caatccccat ccggcgagaa aaaaacatcg gcccggtggc gttgcaaagc cataggaacc
    11881 gcccattcaa accaggcgta ccacaacaaa gggtgccggg ccggcggcgc tgctaccacg
    11941 ggcttcacgt tgccggaagg cagccaggcg gaatcgaagg ggcggtcgaa taagaagata
    12001 aactcgtcat cgggaaaacg atccaccaag cggcgggcga cttcccaggt aaaccgcccg
    12061 ataccttcca tgcggccggg cagcaaaaaa cgagcattaa cggcaatgcg cattacgagc
    12121 cgggacctgt gacgggcggg gcaatggatt tgagttcgcc ccatttttcg gttttaccgt
    12181 cggagcggcg caccaaaata cctttggcag catagccggc tttggccacg cgctctttta
    12241 tttcgctcat ttgcattttg taacggggcg gcatggtaaa agtcatttta ccggttttga
    12301 aatcggcttt tacctgagca atgcctttga cggctttgaa tttggtttcc aaaccgccgg
    12361 agcatatcgg gcaggtaagc ccctccacat acaccgtaaa ctgatcggtt tgggcctgag
    12421 cggctcccgc agctatgaaa agcagggcca aaaagagaag atttttcatg tcggacaaat
    12481 ataagtgcgc ggtttagtga aggggtgacg gccagtcgcc ccttcaccaa gctaaacgcc
    12541 ataaaaacac ctcgtatccg gcgccggttg catccaacga caaagggcga gaggaatcac
    12601 tccgcccgcc cttcgcgcat tcttgaatta caaaaaaact tagccgatta cagcattgtc
    12661 cttcgccatg gtcttggcat acat
//
LOCUS       DAORLF010000076        12675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2642_length_12675_cov_2.220444, whole genome shotgun sequence.
ACCESSION   DAORLF010000076 DAORLF010000000
VERSION     DAORLF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_12675_cov_2.220444"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PK198_08365"
     CDS             <1..319
                     /locus_tag="PK198_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38788.1"
                     /translation="NPKSGIRNQESEIRNPKSLLPPWAFFAAFAFLVFFAALLLSAFF
                     FQTDRTAGAIAGAGFQQRCVVLIGLHESFICGARYSAGHSVNMTNLTRCNDFLQYRLK
                     IGQ"
     gene            547..>2173
                     /locus_tag="PK198_08370"
     CDS             547..>2173
                     /locus_tag="PK198_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF38789.1"
                     /translation="MKKTLLLLLLMLGLGAAYAQRATVSGKVTDAQTKEAVIGANVYL
                     EGTTSGTVTDEDGMYSFTAAPGDYTLIISYVGYGNQSQKISLMAGKDITVDVMLETTN
                     LVGQEVVVSASRSAEKITNAPATISVINARSIAELPSFNVAELLGRQRGLDYVRSGVL
                     GIGVNARGFNSAFNPKNLQVNDGRFSTLIATGLPLGALSTTVKEDVERIEVVLGPSSA
                     LYGPNAHNGLINTITKNPRTSQGTTVAMGFTSSLQDGLFGETPHAMSYRLRHAMKLGE
                     KLSFKVSGEYTQGQEFNFIDSVYIGVVGFNEFGSDFDNEGLQRPDFGFNSLRGEAQVY
                     YAVTPKSDIIASYGGSKSNNIGNTNAGRNQIKDWLVNWAQLRYVSPRFFAQVYNTWSS
                     TDSTFAMNQRTVNYWTFRNNGFSAEDARVKSFTKQWFPIAGFPGGGLELNRGSIFQDR
                     SQRFNAEAQYNNRLGNVLSYIVGVQYQRDAANSNGTYLLDKNADGEIEDIVIGQLGGY
                     LQADLKFGTNFKAVLAARADQHDLYGFNFIPKAA"
     assembly_gap    2174..2242
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2243..3108
                     /locus_tag="PK198_08375"
     CDS             <2243..3108
                     /locus_tag="PK198_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF38790.1"
                     /translation="NLEANIFGGVLLGNGAGFTLSDGTKINPLKVETISTLEAGYKGQ
                     LSNKLFVDANAYYNWSKDFLSPALNIATNGRTVTHRGDVPMSQVVPGTPATGSTLVLT
                     YLNFGDVNTYGFDASVSYSLSSSLLATLNYSYFGYAIDTTDLANDGNRNGKVDENDLP
                     INTPTHKLGLGLNYTSGKFFGSLFGRWVDQYDFFSGINVAAATNTSLIYSGSPVVENA
                     RVGRSFNNGPLGGFFNLDLSAGYRISDIFTVSGQVTNLLNAEVREFVASPAIAPLYSL
                     ELKVNLPAIGK"
     assembly_gap    3223..3297
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3298..4221
                     /locus_tag="PK198_08380"
     CDS             <3298..4221
                     /locus_tag="PK198_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRF38791.1"
                     /translation="NELLGEDVDAWLRTNVEIYERRWCRPDESTADLCEKAALQALER
                     AGITADQLQLLIIATDTPEYVSPSTASVVQDRIGAVNAGTFDVNTACAGFVTALDIAS
                     KYILSDEQYQHVLVIGAYAMSKYLNMADKKTVTLFADGAGAVVMAAEGGEQKRGFLSS
                     QLISRGQYCEWMGIYGGGTKYPVSEQVLADKTHLLQFVKKFPKELNPEIWSAMTRTLC
                     ERIGATPDDIDHFFITQINVHSLRETMDLLGQPHEKAHTAMHYLGYTGSACIPMAFNL
                     AMEAGKVKPGQLGFFIGSGGGLAFAAAAVRF"
     gene            complement(4222..4812)
                     /locus_tag="PK198_08385"
     CDS             complement(4222..4812)
                     /locus_tag="PK198_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF38792.1"
                     /translation="MNYHAAKAFIVEKLERELSADLTYHGLHHTLDVLHITEELCLME
                     SIGVYESMLLKTAALFHDSGFIKNNKDHETLGCAIAREHLPRYDYTPLEIERICSMIM
                     ATKIPQSPANFLEAILCDADLDYLGREDFFDIGSTLFQELKTYQVLQREEDWNRLQVS
                     FLEKHAFFTPTNQRRRAPRKQVYLEQLKQVVAGYDL"
     gene            complement(4947..5441)
                     /locus_tag="PK198_08390"
     CDS             complement(4947..5441)
                     /locus_tag="PK198_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRF38793.1"
                     /translation="MLKYLLFALMLLPVAVVAQSGAKAAKGNPQGSVFGEAFKAKGAM
                     PYDQMLNRMAGKEKLPVKVRGTVDNVCQVKGCWMNIVSTQDGKEKMMVKFKDYGFFMP
                     KDIAGREVILDGYAFYEVTSVDELRHYAEDAGKTKEEIALITEPSKKLMFMASGVLLL
                     DKAK"
     gene            5734..7236
                     /locus_tag="PK198_08395"
     CDS             5734..7236
                     /locus_tag="PK198_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF38794.1"
                     /translation="MMRAMLSLLAALACLTPSAAQFKCLSGNCFNGFSECVYPSGARF
                     AGYFEDGRIHGQGTILFSNGDRYTGEWKNHQREGKGEFVFADGRKYTGDFLRNRQHGT
                     GTLLYPNGDRYEGQFADGVRQGRGILYHADGSTLSGMWEMDQYRPEWGKTVVLGDTAN
                     LRNCNAVPCPEGVGKYSYRDGSRYYGQFIKGNPEGNGSVYYPGGDRYEGEWKNHAPNG
                     QGVMYYKSGKVLGAVWNAGKPARELFYESEHFDRGGIKIERSSEVKIWAIVVGVAQYN
                     HMPTLRYTDDDAYQLYAFLKSPEGGALPDQQIQILVDEDATRVNILNAMKAVLLRADE
                     NDVVLFYFSGHGLQGCFLPADYDGHNNKLYHQEVREVLLASRAKHKLVLADACHAGSL
                     MAMRNGVVGSALQTYYEAFDKSEGGLALLLSSKGEEYSLEDGGLRSGVFSHFLIRGLK
                     GEADVDRNKIVTVQELHDFTYERVRRYTAGVQSPVLSGLFDKHMPVAIVR"
     gene            complement(7244..8203)
                     /locus_tag="PK198_08400"
     CDS             complement(7244..8203)
                     /locus_tag="PK198_08400"
                     /inference="COORDINATES: protein motif:HMM:NF018339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HRF38795.1"
                     /translation="MSKIAEARQILEALGLPKEQQNERSALTLLALCHLKEEDAWSSA
                     KAVSMSVVGNKENARYEGIMRFIARHYHKQYAENSRETIRRQTLHQFVQAGIVLHNPD
                     NPELPTNSKDNHYKLSPVALKVVKTYGGENWSKEVARFRKNVGLLRDKYNRERVLQQI
                     PVKLRDGLELNFSPGKHNQLQVAILEDFAPRFAPGSSLLYVGDTARKYLFIDESGLKN
                     LCIPIDEHGKLPDVILYDQKRNWLFLIEAVTSHGPVSPKRIIELEALFQHCPAGKVYV
                     TAFPDFKEFKKHSADIAWDTEVWLSEMPGHLIHFNGDRFLGPR"
     gene            complement(8200..9777)
                     /locus_tag="PK198_08405"
     CDS             complement(8200..9777)
                     /locus_tag="PK198_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRF38796.1"
                     /translation="MDNSIAIPNIASELPSRVAERLGLAYASSVSSQHKKENGQFFTP
                     IGIAEFMASCSDYRSSKARVLDPGCGTAVLSCAWVERMVLAGTLRQVELTAYETDTDL
                     TPYTRQSLDYLEKWCAERGVRISTTLYTDDFVMRNADALEAAPGLFSPQPELFDVVLS
                     NPPYFKLPIDDKRAVAAKIIVNGHPNIYAIFMALSAKLLQEEGELIFITPRSYAAGSY
                     FKTFREYFFRLVDLERAHLFVSRKETFSRDKVLQETVIIKAVRRNISGPEVVISSSCG
                     VKDLAYPSVKVFPQKDIIDINSKEKILYLPVSDKEEAILEVFKYWTGSLSKYHIRIST
                     GPVVAFRAVQYISGAAAPETVRLAPLFWLHNVRPMQLEWPVTKPNKGQYIHIEGVPAS
                     LLIPNKNYVLLRRFSSKDDKSRLIAAPYFANDVQSDWIGVENKVNYIYRAEGELEANE
                     VMGFCALLNSRLFDAYFRIFNGNVNVSATELREMPLPPISMIRAIGDALLQSDDISVE
                     HADRIVAQYFEPAEYSL"
     gene            complement(9817..11601)
                     /locus_tag="PK198_08410"
     CDS             complement(9817..11601)
                     /locus_tag="PK198_08410"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HRF38797.1"
                     /translation="MLYQNPISKPPLYIVRQGNAAILEYFRQKEKTGAELLLEAKLIL
                     LGDGRAGKTSLANRLLGKNLPAEADRTQGVDIIIGEYEFPVAGGGTFQLNIWDFAGQD
                     KYKPLHQFFYTESSLYVMVAESGNSGTDYDDWFQTAELFGEGSPLLLALNEFRDGTGL
                     GSFDVERWKKRFPSLLKEVFSVNLGTQRGMEELRQHIHLLAQTLPHTRYEFPKNWAAV
                     RRELESRRSEHFISLQEYLRICRENQLPERDSALILSSVLHKIGVCLHYQKSELLRQY
                     VILNNEWATGAVYQILEDPVVAEEKRGFFDWNDLRRIWHDAAYCDMQPQLLELMQQFK
                     MAYPLPNGKEYVAPPLLSPAPPANWECPAGESLAMYVEYEFMPKLLMTQFIVSRYMDI
                     AEGRTQVWRNGVALRWPDALAEVVKSKSQGRDAFLIRVQGKGGKGLLTAIRNTLRDLH
                     DEYRGIRPHEIIPCPCSGCRSGKNRQHYFDYENLQNRLEKGRRVVECDKSLEELNLLE
                     LLGALFVFDSLEVGRPLRMREVASPGALERQGLEESLHLMIQKLNRIREAFAIENDPS
                     AKFKYEKQIEALEQEIAEVRRKLRRMNG"
     assembly_gap    12161..12170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12171..12353)
                     /locus_tag="PK198_08415"
     CDS             complement(<12171..12353)
                     /locus_tag="PK198_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38798.1"
                     /translation="MSELAQQLIEREKELRTGYLDLGNCGLTELPDLSELEWLETLVV
                     SNGWVDEENGEWVKSQN"
     gene            complement(12438..>12675)
                     /locus_tag="PK198_08420"
     CDS             complement(12438..>12675)
                     /locus_tag="PK198_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRF38799.1"
                     /translation="PGSTLSAAEAISVVTNGQALAVHFGDGVIKAADVAAGLTGAIIK
                     DPTQDAIVWKEYLEVVVKERSEWKDLYRACKDLL"
BASE COUNT         2885 a         3391 c         3185 g         3060 t
ORIGIN      
        1 gaatccgaaa tcaggaatcc gaaatcagga atccgaaatc aggaatccga aatcactcct
       61 acctccctgg gcattttttg cagcgtttgc tttccttgta ttttttgcag cacttcttct
      121 ttctgccttc tttttccaaa cggacagaac agcgggcgca atcgccggcg caggttttca
      181 gcaaaggtgt gtggtcctta tcgggttgca tgaatcgttt atttgcggcg caaggtatag
      241 cgccgggcac tcggtcaata tgacaaatct cacgcggtgc aatgattttt tgcaatatcg
      301 tctaaaaatt ggtcaatagg gtaattttat aaattaaaaa actgataatc atttatttaa
      361 cacaaacaat tattattttt taaaaaagcg tcaaccgcca gattgtacat cccggcccat
      421 caaaaaacaa gccgcttgct aatttcgtcc tgccgaataa atttaagcgt tatttagccc
      481 gtggcatgaa aaagcgccaa aatttaattg cagcccaata tttattccaa ttaaaacagt
      541 tttgacatga aaaaaacact tctacttctt ctgctgatgc tgggcctcgg agctgcctat
      601 gcacagcgcg caacggtgtc gggcaaagtg accgacgccc aaaccaaaga agccgttatc
      661 ggcgccaacg tttacttgga gggtactacc tccggcaccg tcaccgatga ggatggcatg
      721 tacagcttca cggctgcacc gggcgattat accctcatca tttcttacgt cggctatggc
      781 aaccaatctc aaaaaatctc cctcatggcc ggcaaagaca tcactgtaga tgtgatgctg
      841 gaaacgacca acctcgtagg tcaggaggtg gtggtgtcgg cttcgcgctc ggcggagaaa
      901 atcaccaacg cgcctgctac catctcggtc atcaatgctc gttccattgc ggagttgccc
      961 tccttcaacg tggcagaatt gctgggccgt cagcgcggtt tggactatgt tcgctcaggc
     1021 gttcttggca tcggcgtcaa tgcgcgcggt ttcaacagcg cctttaatcc caaaaactta
     1081 caggtgaacg acggccgctt ctctacgctc atcgccaccg gcctgccgct gggcgcgctc
     1141 agcaccaccg tcaaagaaga tgttgaacgc attgaagtag tgcttggccc ctcttcggcc
     1201 ctgtatggcc ccaacgcgca caacggtctt atcaacacca tcactaagaa tccgcgcacc
     1261 agccagggta ctaccgtagc catgggcttc accagctcgc tacaggacgg cctctttggc
     1321 gaaacgccgc acgccatgag ctaccgcctg cgccacgcca tgaaacttgg cgaaaagctc
     1381 tctttcaaag tgagtggaga atatacgcag gggcaggagt tcaattttat tgattccgtg
     1441 tatatcggcg ttgtcgggtt caacgaattc ggctctgatt tcgacaatga aggcttgcag
     1501 cgacctgact tcggcttcaa ctcgcttcgt ggcgaggctc aggtttacta tgccgtaacg
     1561 cccaaatctg acatcatcgc ttcttatggc ggcagcaaga gcaacaacat cggcaacacc
     1621 aacgcaggtc gcaatcaaat caaagactgg ctcgtcaact gggcgcaact tcgctatgtg
     1681 tcgccccgct tcttcgcaca ggtttacaac acctggagca gcaccgactc taccttcgct
     1741 atgaaccagc gtacggtgaa ttactggact ttccgcaaca acggcttcag cgccgaagat
     1801 gcccgcgtca aatccttcac caagcagtgg tttccgattg ccggtttccc cggcggcggt
     1861 ttggaactca atcgaggttc cattttccag gacagatcgc agcgcttcaa tgcagaagca
     1921 cagtacaaca accgtctcgg caacgtcctg agctacatcg taggcgttca gtatcagcgc
     1981 gatgcagcca acagcaacgg tacttacttg ttggataaaa acgcggatgg tgaaattgaa
     2041 gacatcgtca ttggtcagtt gggcggttat ttgcaggccg atctcaaatt tggcaccaac
     2101 ttcaaagccg tgctcgctgc ccgcgccgat cagcacgatc tgtatggatt caacttcatt
     2161 cccaaagcag cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nntcaacctc gaagccaaca tcttcggcgg cgtactgttg
     2281 ggcaacggcg caggctttac cctctctgac ggaacgaaaa taaacccgct caaagtggaa
     2341 accatcagca cattggaggc cggctacaaa ggccagcttt ccaataagtt gtttgtggat
     2401 gccaatgctt actacaactg gtcgaaagac ttcctcagcc cggcgctcaa catcgctacc
     2461 aacggacgca ccgttaccca tcgcggcgat gtgccgatgt cgcaagtagt tccgggtaca
     2521 cccgctacgg gttcgacatt agtgcttact tacctcaatt ttggtgatgt aaacacttac
     2581 ggcttcgatg catcggtgag ctacagcctt tcttccagct tgcttgccac actcaactac
     2641 tcttatttcg gctacgccat tgataccacc gacctggcca acgacggcaa ccgcaacggc
     2701 aaagtggacg aaaacgacct gcccatcaac acgccgaccc acaaactcgg cttgggcctg
     2761 aactatacga gcggcaagtt cttcggctcg ctgtttggcc gttgggtaga tcagtacgac
     2821 ttcttctccg gcatcaacgt ggcggcagct accaatacca gcctgattta cagtggctcg
     2881 ccggtcgtag aaaatgcccg cgtaggtcgc tccttcaaca acggcccgct gggcggcttt
     2941 ttcaacctcg acctctccgc cggttaccgc atcagcgaca tcttcaccgt gtcgggccag
     3001 gtgaccaacc tgctcaacgc cgaggtgcgt gagttcgtag cttctccggc catcgcgccg
     3061 ctgtactcgc tggaactcaa agtgaatttg cccgcaatag gcaagtaatt tttatcatct
     3121 gagagctaaa atgagcctgc cgacaaggta ggcagggctg tgagccgcga gctgagactc
     3181 gtggctcgca gctcatagct cgcagctacc aaactcatct tcnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac
     3301 gaactattgg gcgaagacgt ggacgcctgg cttcgtacca acgtagaaat ttacgaacgc
     3361 cgctggtgcc gccccgacga aagcaccgcc gacctctgcg aaaaggcagc cctgcaagcc
     3421 ctcgaacgcg ccggcatcac cgccgaccaa ctccaactgc tcatcatcgc caccgatacg
     3481 ccggagtatg tttcgccctc aaccgcttcg gtggtgcaag accgcatcgg cgccgtcaac
     3541 gcaggtactt ttgatgtaaa taccgcttgc gcgggatttg tcaccgcctt ggacattgcc
     3601 tccaaataca tcctttctga tgaacagtac cagcatgtgc tcgtcatcgg cgcgtatgcc
     3661 atgagcaagt atctgaacat ggccgacaaa aaaaccgtga cgctttttgc cgacggcgcg
     3721 ggcgcggtgg tgatggcggc agaaggaggg gagcaaaaac ggggtttcct cagcagtcag
     3781 ctcatttcgc gggggcaata ctgcgagtgg atggggattt atggcggcgg taccaagtat
     3841 cccgtcagcg agcaggtgct ggccgacaaa acgcatttgc tgcaatttgt caaaaaattt
     3901 cccaaagaac tgaatcctga aatctggtcg gccatgacgc gcaccctctg cgagcgcatc
     3961 ggtgctacac ccgacgacat tgaccatttt ttcatcaccc agatcaatgt acatagtctg
     4021 cgcgaaacga tggacctgct ggggcagccg cacgaaaaag cgcacacggc catgcactac
     4081 ctcggctaca ccggctcggc ctgcataccg atggccttca atctggctat ggaagccggc
     4141 aaagtgaaac cgggccaatt gggctttttc attggttcgg gaggcggact ggcgtttgcg
     4201 gcggcggcgg tgaggtttta gttataagtc ataccccgcc actacctgct tcagttgttc
     4261 taaatacact tgcttgcgag gtgcgcggcg gcgttggttg gtgggggtaa aaaaggcatg
     4321 tttttccaaa aaagatactt ggaggcggtt ccagtcttct tcgcgctgaa gcacttggta
     4381 ggttttcagt tcttgaaaca aagtagagcc gatgtcgaaa aaatcttccc ggccgaggta
     4441 gtccagatcc gcatcgcaga ggatggcttc gaggaagttg gcggggcttt gcggaatttt
     4501 ggtggccata atcatgctgc aaatgcgctc tatttccaat ggcgtgtaat cgtagcgcgg
     4561 caggtgttcg cgggcaatgg cacagcccag cgtttcgtgg tctttgttgt tcttaataaa
     4621 accggaatcg tgaaacaagg cggccgtttt gagcaacatg gactcataga ctccaatgga
     4681 ctccatcagg cacaactcct ctgtgatgtg cagcacatcc aatgtatggt gcagcccgtg
     4741 gtaggtcaga tcggctgaaa gctcgcgttc cagcttctca acgatgaatg ctttggcagc
     4801 gtggtaattc atggttcgcg tagtttctca tagtatatct gcgctcataa cgctggggaa
     4861 atctgagata gtacgttaac gggttggggc aggaggtaac ttgtgattta cctcctgact
     4921 ttgcccgccg gagactgtct ctctccttac ttagccttgt ccaagagcaa tacgcccgag
     4981 gccataaaca tgagtttttt gctcggctcg gtgatgaggg caatttcctc tttggttttg
     5041 cccgcatctt cagcatagtg gcgcagttcg tccacagagg tcacttcata aaaagcgtag
     5101 ccgtcgagaa tgacttcccg gccggcaatg tccttaggca taaagaaacc atagtcttta
     5161 aacttgacca tcattttttc cttgccgtcc tgtgtagaaa cgatgttcat ccagcagcct
     5221 tttacctggc aaacattgtc cacggtgccg cgcactttca cgggcagttt ttccttgccc
     5281 gccatgcggt tcaacatttg gtcataaggc atagcgcctt tggctttgaa agcctctcca
     5341 aaaacggagc cttgagggtt gcctttggcg gcttttgcgc ccgattgcgc cactactgcc
     5401 acaggcagca gcatgagtgc aaacaacaga tactttaaca tggtactaac gtatgttaag
     5461 tgaaagaata aagcaacagg aacgcccgtt tagatcagca tcttggtttt ttcctgccct
     5521 gcaaaggtat ggattttttt aaatctcgcc gaaagcgcaa caataggccg caacggcagt
     5581 tattacattc aaataaaaaa aaaatctata taaccaaagc cgatttctat tcgatcaacc
     5641 gctacaaatt catgagcagt ctccctcgct gaacgttaac accgaatggc cttccgccgc
     5701 gggtgctgca cgctatctac agtcaaacac cttatgatga gagcgatgtt gagtttgttg
     5761 gcggctttgg cctgcctcac gccctccgcg gcacaattca aatgcctgag cggcaattgt
     5821 ttcaatggtt tcagcgaatg cgtttacccc agcggcgcgc gcttcgccgg ttattttgaa
     5881 gacggccgta ttcatggtca gggcaccatt cttttcagta atggcgaccg ttataccggc
     5941 gaatggaaaa accaccaacg cgagggcaaa ggcgagtttg tgtttgcaga cgggcgcaaa
     6001 tacacgggcg attttctccg caatcgccaa cacggtaccg gcacattgct gtatcccaac
     6061 ggcgaccgct acgaggggca gtttgccgat ggcgtgcgtc agggcagagg tattttgtac
     6121 cacgccgacg gcagtacctt gagcggtatg tgggaaatgg atcaataccg cccggaatgg
     6181 ggcaaaacgg tggtactggg cgatacggcc aacctccgca attgcaatgc agtgccctgc
     6241 ccggaaggcg tgggcaaata tagctaccgc gatggctcgc gctattacgg ccagttcatc
     6301 aaaggcaatc ccgaaggcaa cggctcagtc tattatcccg gtggcgaccg atacgaaggc
     6361 gaatggaaaa accacgctcc caatggacaa ggcgtgatgt attacaaaag cggcaaagtg
     6421 ctgggcgctg tgtggaacgc aggaaaaccc gctcgagagc tgttttatga aagtgaacat
     6481 tttgaccggg gcggcatcaa aatagaacgc agctctgagg taaaaatatg ggccattgta
     6541 gtgggcgtgg cgcaatacaa ccacatgcct accctacgct acaccgatga cgatgcctac
     6601 caactctacg cttttctgaa aagcccggaa ggcggcgctc tccccgacca gcaaatccaa
     6661 atcttagtgg acgaagacgc cacccgcgtc aacatcctca atgccatgaa ggccgtgctg
     6721 ctgcgcgccg acgagaatga tgtggtgctg ttttatttct ccggtcacgg cttgcaaggc
     6781 tgctttctcc ccgctgatta tgacggccac aacaacaagc tttaccacca ggaagtgcgc
     6841 gaggttttgc tggccagccg cgccaaacac aaattggtac tggcggatgc ctgccatgca
     6901 gggtctttga tggccatgcg caacggcgtg gtaggctctg cgctgcaaac ctactatgaa
     6961 gcgttcgaca agtcggaagg cggcctggca ctgctcctct catccaaagg cgaagagtac
     7021 tcgctggaag acggcggcct gcgttcgggg gtgttcagcc attttctcat tcggggcctc
     7081 aagggcgaag cggatgtgga tcgcaacaaa attgtgacgg tgcaggaatt gcacgatttc
     7141 acctacgaac gcgtacgccg ttataccgcc ggggtgcaat cgccggtgct cagcggactg
     7201 tttgacaaac acatgccggt ggctattgtg cgctgataat ctcttaccgg ggtcctaaaa
     7261 atctgtctcc attgaaatgt atcaagtggc cgggcatttc gctgagccaa acctctgtgt
     7321 cccaggcaat gtcggcagag tgctttttga attctttaaa atctggaaat gctgtcacat
     7381 acacctttcc agcggggcag tgttgaaaca gcgcttctaa ttcaataatt cgtttgggag
     7441 acacaggccc gtgagaagta actgcttcta tcaaaaacag ccaatttctc ttttggtcgt
     7501 aaagtataac atccggtagt ttgccatgtt cgtctatagg aatgcagagg ttttttagtc
     7561 cactttcgtc aataaaaagg tacttcctgg ctgtatcgcc cacataaagc aacgaactgc
     7621 cgggcgcaaa acgaggagcg aagtcctcca aaatggcgac ctgtagctgg ttgtgtttcc
     7681 ccggcgagaa attcagttcc aatccgtctc tcagctttac cggtatttgt tgcagtaccc
     7741 gctcccggtt gtacttatcc cgtaacaagc cgacattttt tctaaaccgc gccacctctt
     7801 tactccaatt ttccccgccg taagtcttta ccactttcag agctaccggt gacagcttgt
     7861 agtggttgtc tttgctgttg gtcggcagtt ccgggttatc cgggttgtga agtacgatgc
     7921 cggcttgtac aaattggtgg agtgtttgtc gtcgaatggt ctccctcgaa ttttctgcat
     7981 attgcttatg gtaatgccgg gcgatgaaac gcatgatgcc ttcgtaccgc gcattttctt
     8041 tattgcccac caccgacatg ctcacagctt tggcgctgct ccatgcgtct tcttctttca
     8101 aatgacacaa agcgagcagc gttaatgccg agcgttcgtt ttgctgttct tttggaagtc
     8161 ccaacgcttc caatatttgt ctggcttcgg ctattttgct cataagctat attcggcggg
     8221 ttcaaaatat tgcgctacga tccggtcggc atgttccaca gaaatatcat cggactgcaa
     8281 caaagcgtct ccaatggctc gaatcattga aatgggaggc aggggcattt cccgcaactc
     8341 cgtggcactg acattcacat tgccgttgaa aatgcgaaag taggcatcaa acagccggct
     8401 gttgaggagc gcgcaaaaac ccatcacttc attggcttcc agttccccct ctgcccggta
     8461 gatataattt actttattct caacgcctat ccaatccgac tgtacatcat tggcaaaata
     8521 cggcgctgct atcaggcggc ttttgtcgtc tttactgctg aaacgcctga gcagcacata
     8581 gtttttattg ggaatgagca acgaagccgg cactccctct atatggatgt attgtccttt
     8641 gttgggtttg gtcacaggcc actccaattg catggggcga acattgtgga gccagaataa
     8701 cggcgccaag cgtacggttt caggcgctgc tgccccgctg atgtattgta cagctcggaa
     8761 ggccactacc ggtcccgttg aaattcggat atggtatttg gacaagctgc cagtccagta
     8821 cttgaagact tccaatatcg cctcctcctt gtcggaaacg ggcaggtaca gaattttctc
     8881 tttggagttt atgtctataa tgtctttttg gggaaatact ttcaccgatg gatatgccag
     8941 gtctttcaca ccgcaggatg aagaaatgac cacctccggc cccgaaatat tccgcctcac
     9001 agccttgatg atgacggttt cctgaagcac tttgtcgcga ctgaacgttt cttttcttga
     9061 tacaaaaagg tgcgcccgct ctaagtccac caatcgaaaa aaatactccc tgaacgtttt
     9121 gaagtagctg ccggcggcat aactgcgcgg ggtaataaaa atcaactcgc cttcttcctg
     9181 gagcaatttt gccgacaagg ccatgaaaat ggcatagata ttgggatggc cgttgacgat
     9241 gatttttgca gctacggctc ttttgtcatc aatgggaagt ttgaaatacg gcggattgga
     9301 cagcaccaca tcaaataatt ccggctgcgg tgaaaacaac ccgggcgcag cttcgagcgc
     9361 atcggcatta cgcatcacga aatcgtcggt gtataaggtg gttgagattc ggacgcctct
     9421 ttctgcacac catttttcaa gataatcgag ggattggcgg gtgtagggtg tcaggtcggt
     9481 atcggtttcg taggcggtca gttccacttg gcgcaaggtt ccggccagaa ccatgcgctc
     9541 tacccaggca cacgacaaca ccgcggtgcc gcagccggga tcgagaacgc gggccttgga
     9601 tgaccggtaa tccgaacacg aagccatgaa ctccgctatg ccgatgggcg taaaaaactg
     9661 cccattttct tttttgtgct gcgaagatac agacgaagca tacgcaagcc ccagccgttc
     9721 ggcgacacgg ctcggcagtt ccgacgcgat gttgggtatg gcaatgctgt tgtccatgtt
     9781 gcagttgggt aaaggaatag cgcaaagtta agttttttat ccattcatcc tgcgaagctt
     9841 ccgccgcact tcggctatct cctgttcgag ggcttctatt tgtttttcgt atttaaactt
     9901 tgctgaggga tcgttctcaa tagcgaatgc ctcccggatg cgattgagtt tttgaatcat
     9961 aagatgtagc gactcctcca acccctgccg ctccagcgcc cccggcgagg ccacctccct
    10021 catgcgcagc ggccggccca cttccaggct gtcgaacaca aacaaagctc ccagcaattc
    10081 cagcagattc agctcctcca gcgatttgtc gcactccacc acgcggcggc ctttttcgag
    10141 gcggttttgc aggttttcgt agtcaaaata atgctgccgg ttcttgcccg acctacaccc
    10201 ggaacaagga caagggatga tctcgtgcgg ccggatgccc cggtactcat cgtgcaggtc
    10261 gcgcagggtg ttccggatgg ccgtgagcag gcccttgccg cctttgccct gcacccgaat
    10321 gaggaaggcg tcgcggccct gcgacttgga cttcaccact tcggccagcg catcgggcca
    10381 gcgcagcgcc acgccgttgc gccatacctg cgtgcgcccc tcggcaatgt ccatgtagcg
    10441 gctcacgatg aactgcgtca tcagcagttt gggcatgaac tcgtactcca catacattgc
    10501 aagcgattcg ccggcagggc attcccagtt ggcgggcggc gcgggcgaca acaacggtgg
    10561 ggccacatac tccttgccgt tgggcagcgg gtacgccatt ttgaactgtt gcatcagttc
    10621 caatagttgg ggctgcatgt cgcagtaagc cgcgtcgtgc cagatgcggc gcaggtcgtt
    10681 ccagtcgaag aagccgcgtt tttcctcggc cactacaggg tcttcgagga tttggtacac
    10741 cgcgccggtg gcccactcgt tgttgaggat gacgtattgt cgcagcagtt cgctcttttg
    10801 gtagtgcagg cacacgccta ttttgtgcaa taccgaactg aggatgagcg cgctgtcgcg
    10861 ttccggcagt tggttctccc ggcagatgcg gaggtactct tgcagcgata tgaagtgctc
    10921 gctgcggcga ctttcgagtt cgcggcgcac ggcggcccag tttttgggaa actcatagcg
    10981 cgtgtgcggc aacgtctggg ccagcaggtg aatgtgttgc cgcagttcct ccatgccgcg
    11041 ctgcgtgccg aggttgacgg agaacacctc tttgagcagg ctgggaaagc gctttttcca
    11101 gcgttccaca tcaaaagagc cgaggcccgt gccgtcgcgg aactcattga gcgccagcag
    11161 cagcggactg ccctcgccaa acagttccgc cgtttggaac cagtcgtcgt agtcggtgcc
    11221 gctgttgccg ctttcggcca ccatcacata caggctgctt tcggtgtaga aaaactgatg
    11281 caggggcttg tacttgtcct gcccggcgaa gtcccagata ttgagttgga aggtaccgcc
    11341 cccggccacc ggaaactcat actccccaat gatgatgtcc accccttgcg tgcggtcggc
    11401 ctcggcgggc aagttcttgc ctaagaggcg attggccaag gaggttttgc cggcccgccc
    11461 gtcgccgagg aggatgagct tggcctctaa gagcagctcc gcgccggttt tttctttttg
    11521 gcggaagtat tcgaggatgg cggcgttgcc ttggcggacg atgtagagag gaggtttgga
    11581 gatgggattc tggtaaagca tgacctcccc gtcgctttgt ccttttacgg ccaatcttaa
    11641 gcctctcttt attaatcgat gtaatggtgt aatacactca atattgttat ttcccaaatg
    11701 cagccatttt aaactggtga gcatttccaa gggcttaatg tctcttattc tgttttttcg
    11761 caaatcaaga taagctagtt gtaccaattg ttctaaaggt ttaatgtcgg taatatcatt
    11821 gcgacttata tccacaaagg tcaaactatt aatgtgtctg agagagctaa tatcattaac
    11881 ttgattgccc tccaaatcaa gctgctccaa attcgtcaac tgacccagcg cttcaatatt
    11941 gacaatttta ttgttcttga ggttgagttc ggtcagcttc gccaagctgt taataggtga
    12001 tatatcttta atctgattaa attgcaaatc aagcactgtt aaattatcga gacctactaa
    12061 aaattgcaaa tcatcagtag gccagggatc acgccattgg ccatcccatt ttaaccgctt
    12121 gagtcccata ggtaaatacc cggttggcgg agatccaagt nnnnnnnnnn attttggctc
    12181 ttcacccatt ccccattttc ttcatccacc cacccattcg acaccaccag cgtctccaac
    12241 cactccaact ccgacaagtc cggcagttcc gtcaatccgc aattgccgag gtccaaatac
    12301 ccggtgcgga gttctttttc gcgttcgatg agttgttggg caagttccga catggcgttt
    12361 gtttaaggtg tgcaagatag ggagtttttt tagtagtaca gaccttgcaa ggtttgccaa
    12421 accttgcaag gtctgtacta cagcaagtct ttgcaagctc tgtacaaatc cttccactcg
    12481 ctgcgctcct tcactaccac ctccaaatat tccttccaaa caatagcatc ctgcgtgggg
    12541 tctttgatga tggcgcccgt tagaccggca gccacatcgg ccgctttgat gacgccgtcg
    12601 ccgaaatgca cggccagcgc ctgtccgttg gtgaccaccg aaatggcctc tgccgcgctc
    12661 agggtgctgc ccgga
//
LOCUS       DAORLF010000077        12607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2658_length_12607_cov_2.113129, whole genome shotgun sequence.
ACCESSION   DAORLF010000077 DAORLF010000000
VERSION     DAORLF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_12607_cov_2.113129"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   45..1641
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=833..860
                     /rpt_unit_seq="cttttaatcgcaccttttggaattgaaa"
     assembly_gap    1661..1685
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     repeat_region   1686..1840
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1686..1712
                     /rpt_unit_seq="tttaatcgcaccttttggaattgaaac"
     gene            complement(1870..>6040)
                     /locus_tag="PK198_08425"
     CDS             complement(1870..>6040)
                     /locus_tag="PK198_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRF38800.1"
                     /translation="TTDDTNDGVCNATHCSLREAIRAANQNPGPDTIVFNITGPGPHV
                     IAPITSLPTITGAGTVIDGSTQPGYFPGAIVLVGNGSATPVDGLEIAGANCAIYGLHI
                     RRFPSGIIVFGGSNARIGAAGKGNIIGGNTLGGITVMGNSPGMVIEGNLIGMFPNGTG
                     ADPNEHGIFLDGQLFGAVPNTTVGGANPGERNIISGNRSEGIFARGVSGLSVFGNIIG
                     LNAAETAALPNGRNGVAVTNSSSCTIGNLAGGHNTISGNGINGINLTGIASGGARVQG
                     NRIGADRSGQIARPNAGNGIFLSDAPGTFIHENILSSNVLSGLEAVQSGGLVIRQNLI
                     GLLVSAGLTGGNGRDGISLSNCDNFVIEDNSISTNLQHGIWVQMLPDRASANGVISHN
                     YIGNYSVVPSQPLGNARYGLALVNCANVIVDGLNVIASNGSGGVLISNSADIILEDNA
                     IGTDQAGLFALGNGGPGITVNAMSEGILIGNLGAGNVIAHNQQGGVVVSENSTSCTIS
                     GNSIYCNQIAGIDLTAGANDDMMPPMEICVAPSRITGMAGPFDLIEVFRHNHADCSTA
                     PSCQGRTFLAGTIAGVDGSWSVSGFFSIGDTITATATNLAGSTSEFSLCSIVRPLPQA
                     VADNSGTACPGEEAQLIGTSIPADTGIAFEWSGPGGFTSDSARISGVLSPGEYMLTVQ
                     QGFCRSEPDTTTVTYYPQATGSFQTTLCPGASITVNGTVYNEMNLSGTEVLADAAANG
                     CDSILSVQVNYYPQATGSFQTTLCPGASITVNGTIYNEMNRTGTEILTGAAANGCDSI
                     LSVQVNFYSPATGSFQTTLCPGASITVNGTIYNEMNRTGTEILTGAALNGCDSILSVQ
                     VNFYPLATGSFQTTLCPGASITVNGTIYNEMNRTGTEVLVGAAAHGCDSILSVQVNFY
                     PLATGSFQITLCPGTSITINGTVYNEMNRTGTEILAGAAANGCDSILSVQVNFFPPAT
                     GSFQTTICPGESITVNGTVYSQSNPGGVELLPGASVNGCDSTFTVSISFFSPAGSTLR
                     HGICPGESVIVNGVMYGPNRLSGTEILRGVSVNGCDSTVVIDLFVLPVPETTFEATIC
                     PGTEIVINGRVYGENKTTGTEVFRGAAANGCDSIVHVRLTIDSLILTIRLRAANCSPQ
                     ERGQITFFSIEGGTAPYAVALDGASFAAINDFPYIINNLAAGPYQLTFRDHFGCLYRE
                     GVTIPDNPLFNISRDTVVEIRRGQTVQLGYGFSFVPDWIRWSPPAGLSCDDCTQPETT
                     PLYSTTYEMDAEFFGCTARQTVRILVDTRVPVYAPTGFTPNGDAVNDRFTLFATPGDL
                     TRIISLKVFNRWGGMMYDGKDLIPGDITSGWDGTFDGRDCPSGMYVFMAEVETAYGEV
                     IALKGELSLVR"
     assembly_gap    6041..6053
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6388..7299
                     /gene="gldA"
                     /locus_tag="PK198_08430"
     CDS             6388..7299
                     /gene="gldA"
                     /locus_tag="PK198_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRF38801.1"
                     /translation="MSVSVQQLTKIYGEQHAVHEVSFEAHSGQVLGFLGPNGAGKSTT
                     MKIITGFLPPSSGTASVCGYNVETAPMEARRRIGYLPENNPLYKEMYVREYLHFTGKL
                     HKVPRLKQRVEEMIEATGLGRECHKLIGALSKGYRQRVGLAQAMLHDPEVLILDEPTS
                     GLDPNQLVEIRALIRQLGQQKTVIFSTHIMQEVQAVCDRVIIINRGKIVADDPIERLQ
                     ERIAGESVLTIEFRQAVKKEALLAIPGVKQAKNTGGNRWQLSAAADADVRPQVADFVK
                     RHELDIIEMHREAISVEEVFKKLTSHA"
     gene            7292..>7880
                     /locus_tag="PK198_08435"
     CDS             7292..>7880
                     /locus_tag="PK198_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38802.1"
                     /translation="MLKYYFIVFQLFAVCLNAQTDSVLVTKNFKFNDGVYLSFADWRN
                     NRPNWTWDSLDVVSATNPNTFLTQVESIRFADGRPMNLDSIWGLVISGIPYVRLPKGA
                     VPKTLTSFAGLRVRGRIAYYNYEVTEEEMVEIRAYNPVTGVPFRKGKVKKQSKALREF
                     MIHFERGETAAFTRENLMAWIADDERLVEAVAALPE"
     assembly_gap    7881..7890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7972..8703
                     /gene="gldF"
                     /locus_tag="PK198_08440"
     CDS             7972..8703
                     /gene="gldF"
                     /locus_tag="PK198_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRF38803.1"
                     /translation="MWSIFVKEIAGFFSSLIGYVVVGLFLLFLGLGLFVWPNSSILDN
                     NYATLNEFFQTVPTVFAFLIPAITMRSLAEENQSGTIELLATRPIRDGEIVLGKFLAA
                     LALTCIALLPTLLYYFTVYQLGDPKGNLDAGAVAGSYIGLLLLAAAFTAVGLLASALN
                     NNQIVAFLMAVLLCGLLFWGFDLISSLPVFAGRGDDVVQQIGMSAHYASISQGVIDSR
                     DVVYFSSIILLFLAFTAAAVNSRKW"
     assembly_gap    8939..9054
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9055..>9323
                     /locus_tag="PK198_08445"
     CDS             <9055..>9323
                     /locus_tag="PK198_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38804.1"
                     /translation="ENSLVEYEFEDPAAGTNDEVSDRRKQLAEDGIRPINLRLKDRQG
                     TSERLIYPYAVFYYKGRNIVVNLLENEVPGVPPEVILNNAVELLE"
     assembly_gap    9324..9403
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9404..10481
                     /locus_tag="PK198_08450"
     CDS             <9404..10481
                     /locus_tag="PK198_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRF38805.1"
                     /translation="PLETADLEKSLRPYYDTGRLNLDSMSSIPQEVAALVIAKPRYPF
                     SEKSKFKLDQYVMNGGKVLWLIDALRIDMDSLRTRERYLATEYTLNLDDLLFQYGARL
                     KTNLVLDYRSTGIQLVTGIVGNAPQFDYFKYPYHVVALPSVNHPIVKSLDGVNLQYVG
                     AIDTIKTKTPVKKTVLLESSQRSMLQYSPVTMDFEFLRYDLDSSKFNKQNLPMALLLE
                     GTFSSLYENRMEQDMLNMLQQIGKPYRAESLPTRMIVVADGDVAKNKINIEKQSYSPL
                     GYNEVDRYLFANKGFVINSLEYLLDEQGVAEARGREVKLRMLDTVQAQEEKTKWRLIN
                     IALPLALLALFGWVFQWWRKRRWS"
     assembly_gap    10759..10788
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10953..12203)
                     /locus_tag="PK198_08455"
     CDS             complement(10953..12203)
                     /locus_tag="PK198_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRF38806.1"
                     /translation="MNTYQTDICIVGAGPGGVAAALQLSYLGIPCIVADKARFPRDKI
                     CGDAISGKIPTLLRRLDPDILARFDALSNRHVGVWGIRFVGPNRKILDIPFYNSERND
                     QATSPGYVCRRMDFDQFLIEELKRRDNIVFLEGTEITHFEKTDSGFTLSDAGGSLRID
                     CRLLLAANGAHSAFSRKYAGLEKDPAHHAAAVRAYYRGVTGFREGNFIELHFLKSVLP
                     GYLWIFPLPDGGANVGLGIRSDIVSRKRINLRKELLRLIEEEPDIRGRFEHAAIEGDI
                     EGYGLPLGSKERRLSGDGYMLLGDAGHLIDPFTGEGIGNAFYSGIIAGQLAQKCLEAN
                     DFSAEFMKAYDVRVARVLGSEMKLSYKLQRMLTYPVVATLLANIITGNAPLIQSLSKM
                     YTDFELRLKLANPLFWLRMLVKKR"
     gene            12293..>12607
                     /locus_tag="PK198_08460"
     CDS             12293..>12607
                     /locus_tag="PK198_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38807.1"
                     /translation="MIRSLLPAAFFWMMFTAATCERPVDLEILLPEPELVIISTFSPQ
                     QPFRATVSSTRPALPPTPTTYIANARVELFQSDNFLQNLQPAPGTLAPYYEALEAVPS
                     EEQ"
BASE COUNT         2942 a         3273 c         3226 g         2892 t
ORIGIN      
        1 aattgaaaca ttgacaaaaa aaaaagagac gcgcaggccg aaagctttta atcgcaccta
       61 tttggaattg aaacagcagt acgccgccgg gtatttttac tggggcctgc ttttaatcgc
      121 acctatttgg aattgaaaca actctcgatg tccgcttcgt cggtgtcgtc ggccttttaa
      181 tcgcacctat ttggaattga aacgcccgtc gcccgtcgtc tgtcggccaa gtacgatctc
      241 ttttaatcgc acctatttgg aattgaaact actttgagat tagtatctcg attgggtctg
      301 cattctttta atcgcaccta tttggaattg aaacgcatgg agggtggggg aagtgacgac
      361 gcgcctgcat cttttaatcg cacctatttg gaattgaaac acgtagaagg agaccgggaa
      421 cagaaacgcg acacaccttt taatcgcacc tatttggaat tgaaacattt ctgcccactg
      481 cattgctcca atccattccg gcttttaatc gcacctattt ggaattgaaa ccctgatcgc
      541 cgccgatata gatgtacgga acttcagctt ttaatcgcac ctatttggaa ttgaaacttt
      601 taaaaaggtg ccgaaaatga aattccttac cttcttttaa tcgcacctat ttggaattga
      661 aactcttcca aatgcactgc cactaacaaa gcattcccgc ttttaatcgc acctatttgg
      721 aattgaaaca actttgcctt taccccatta cacaggtgat tgcccctttt aatcgcacct
      781 atttggaatt gaaacaagac ggtcacgcaa aattcggcct tgacagagaa gccttttaat
      841 cgcacctttt ggaattgaaa cgtctaaggt ttgaccggcg atttcggcgt agatcacttt
      901 taatcgcacc ttttggaatt gaaactgcac agtaaaatat tcgtatgcac ttgcgaacag
      961 tgcttttaat cgcacctttt ggaattgaaa cgtaccttgc gcgaagactt acggaggcaa
     1021 tttctacttt taatcgcacc ttttggaatt gaaacatcgc gttatgacaa gccccgatgg
     1081 tattacatgg acttttaatc gcaccttttg gaattgaaac taaaagtgat ggacaacaac
     1141 ggcaccgggc aaatacccct tttaatcgca ccttttggaa ttgaaactac aattacaata
     1201 ctattgatga acttctcact aatcctttta atcgcacctt ttggaattga aactgtggta
     1261 cgcttcacat caaaccaagc ctcgcggcct tttaatcgca ccttttggaa ttgaaacatg
     1321 tcatgctttc ctgcctgccg gtgggcaaaa gaacttttaa tcgcaccttt tggaattgaa
     1381 acccataagc cactgaagtc catccatctg attctactgc ttttaatcgc accttttgga
     1441 attgaaacaa agctcgtctg aaacagtgtc taagttgtga taaactttta atcgcacctt
     1501 ttggaattga aactttacgc agcgatacac gaatgaattt attcttcact tttaatcgca
     1561 ccttttggaa ttgaaacgcg ttaccctgac gtggaacctg agcgcttacg ctccttttaa
     1621 tcgcaccttt tggaattgaa actccttgat ccagttgcga nnnnnnnnnn nnnnnnnnnn
     1681 nnnnntttaa tcgcaccttt tggaattgaa acttttaggc gtgaccttga aatcctcgga
     1741 taccggcttt taatcgcacc ttttggaatt gaaacaaaaa aactggtaag ccatgaaaaa
     1801 gagatttgga gcttttaatc gcaccttttg gaattgaaac gtacagactt cggcccgcac
     1861 attaccgcct cacctcacca aactcagttc ccccttcagc gcaatcacct ctccgtaagc
     1921 cgtttccact tccgccataa agacgtacat gccggatgga caatctcttc cgtcaaacgt
     1981 gccgtcccaa ccggaggtaa tatcgccggg tatcaggtct ttgccgtcgt acatcatgcc
     2041 gccccagcgg ttgaagactt ttaaagaaat gatgcgcgtc agatcgcccg gcgtggcaaa
     2101 caaggtaaag cggtcattca cggcgtcgcc attgggcgta aaacccgtgg gagcatacac
     2161 cggcactctg gtatctacta aaatcctgac ggtttggcgt gctgtgcagc cgaagaactc
     2221 cgcgtccatt tcgtaagtgg tagagtacag cggcgtggtt tccggctggg tacaatcgtc
     2281 gcaactcaga ccggccggcg gcgaccagcg aatccaatcg ggcacaaaac tgaagccata
     2341 gcccaactgc accgtctgcc cgcgcctgat ttccactacc gtatccctgc tgatattgaa
     2401 aagcggattg tcgggaatcg tgacgccttc tctgtacaag catccgaaat ggtctcggaa
     2461 ggtaagctgg tacgggcctg ccgccaggtt gttgatgatg tacggaaaat cgttgatggc
     2521 cgcaaacgaa gcaccgtcta aggcaaccgc ataaggcgcc gtgcctcctt cgatggagaa
     2581 aaaggtgatc tgcccccgtt cctgcggaga gcaattggcc gcccgcagcc gtatggtgag
     2641 aatgagcgaa tcaatggtga ggcgaacgtg tacgatggag tcgcagccat tggccgcagc
     2701 ccccctgaac acctccgtgc cggtggtttt gttctcgccg taaaccctgc cgttgatgac
     2761 gatttcggta ccggggcaaa tcgttgcctc aaaggtagtt tcgggcaccg gtagtacaaa
     2821 aaggtcaatg accaccgtgc tgtcgcaacc attcaccgag acgccgcgca atatttcggt
     2881 accgctcagg cggttggggc catacatcac gccgttgaca atcaccgact cgccggggca
     2941 aatgccgtgc ctgagcgttg agccggcagg gctgaaaaag ctgatgctca cggtgaacgt
     3001 actgtcgcaa ccgttgaccg aagcacccgg cagcaactct acgccgcccg gattggactg
     3061 actgtaaacc gtgccgttga cggtgatgga ttcgccgggg cagatcgtgg tttggaaact
     3121 gcccgttgcc ggcgggaaga aattgacctg cacgctcaga atgctgtcgc agccattggc
     3181 cgccgcgcct gccagaattt ccgtgccggt tcgattcatt tcgttgtaaa ccgtgccgtt
     3241 gatcgtgatg gatgtgccgg gacaaagcgt gatttggaaa ctgcccgttg ccaatggata
     3301 aaaattgacc tgcacactca gaatactgtc gcaaccatgc gccgccgctc ccacaaggac
     3361 ttccgtacct gtgcggttca tttcattgta aatcgtgccg ttgacagtga tggatgcacc
     3421 gggacaaagc gtggtttgga aactgcccgt cgccaatgga taaaaattga cctgcacgct
     3481 cagaatactg tcgcaaccat tgagcgccgc acctgtcaga atttccgtac ctgtgcggtt
     3541 catttcgttg taaatcgtgc cgttgacggt gatggacgcg ccgggacaaa gcgtagtttg
     3601 gaaactgccc gtcgccggag aataaaaatt gacctgcacg ctcaaaatgc tgtcgcaacc
     3661 attggccgcc gcgcctgtca gaatttccgt gccggttcga ttcatttcgt tgtaaatcgt
     3721 gccattgacg gtaatggacg cgccggggca aagcgtggtt tggaaactgc ccgtcgcctg
     3781 tgggtagtaa ttgacctgca cgctcagaat actgtcgcag ccattggccg ccgcgtctgc
     3841 caggacttct gtgccactga gattcatttc gttgtaaacc gtgccattga cggtgatgga
     3901 cgcgccgggg cagagcgtgg tttggaaact gccggttgcc tgtgggtaat aagttacagt
     3961 cgtggtatcg ggttcagaac ggcaaaagcc ctgttgcacg gtgagcatgt attcgccggg
     4021 ggacaaaacg ccgcttatcc gggcgctgtc ggatgtaaat ccacccggcc cgctccattc
     4081 aaaagcaatg cctgtatcgg cgggaatgga cgtgccgatg agctgcgcct cctcgcctgg
     4141 acaggccgtg ccgctgttgt cggctacagc ctgaggcagg ggccgaacga tggaacaaag
     4201 cgaaaactcg gaagtgctgc cggccagatt ggtagccgta gcggtaatgg tatcgccgat
     4261 gctgaaaaag ccggaaacac tccaggagcc gtccacgccg gcgatggttc cggccagaaa
     4321 agttcgtccc tgacaggagg gcgccgtgct gcaatccgca tggttgtgcc ggaacacttc
     4381 tatcagatca aaaggtccgg ccatgcccgt gatgcgcgag ggtgcaacgc agatttccat
     4441 cggcggcatc atatcgtcgt tggcgcccgc agtgaggtct atgccggcta tctgattgca
     4501 ataaatgctg tttccgctga tggtgcagga ggtagaattt tcgctcacca ctaccccacc
     4561 ctgctgattg tgcgcgatga cattgccggc gcccagattg ccgatgagaa tgccttccga
     4621 catggcgttg acggtgatgc cggggccgcc gttgcccaat gcaaacagcc cggcctgatc
     4681 ggtgcctatc gcattgtctt ccaaaataat atctgccgag ttgctgatga gcacaccgcc
     4741 gctgccgttg gaggctatga cgtttaaacc atccacgatg acgttggcgc aatttacaag
     4801 cgccaggcca tagcgggcat tgccgagcgg ctgtgaggga acaacactgt aattgccgat
     4861 ataattgtgg ctgatgactc cattggcgga ggctcggtcg ggcagcattt gcacccagat
     4921 gccatgttga agattggtag aaatactgtt gtcttctatc acaaagttgt cgcaattgct
     4981 gagcgagatg ccgtcgcggc cattgcctcc cgttaggccg gcagaaacca gcagaccgat
     5041 gaggttttgg cggatgacca gcccccctga ctggacggcc tccaatccac tcaacacatt
     5101 ggaagacaat atattttcat gaataaaagt gcctggcgca tccgacaaaa agatgccgtt
     5161 tcctgcattg gggcgggcta tttgccccga acggtcggcc cctatccgat tgccttgcac
     5221 cctggcgccg ccggaagcga tgcccgtgag attgatgccg ttgatgccgt tgccggaaat
     5281 ggtattgtgc ccgccggcga ggttgccgat ggtgcaggag gaggagttgg taaccgccac
     5341 cccgttgcgc ccgttgggca gggccgcagt ttcagcagca ttcagtccga tgatgttgcc
     5401 gaacacagac agcccggaaa cgccgcgcgc aaagatacct tccgatctgt tgcccgaaat
     5461 gatgtttctt tcaccggggt tggcgccacc gaccgtagta tttggcaccg cgccgaacaa
     5521 ctgtccgtcg aggaaaatgc cgtgttcatt ggggtcggcg ccggttccgt tgggaaacat
     5581 gccgatcaga ttgccctcaa taaccatgcc cggcgaattg cccatgaccg taatgccgcc
     5641 caatgtattc cccccgatga tatttccttt tcctgccgcc ccgatacggg cattggagcc
     5701 cccaaaaaca ataatgccac ttggaaaccg gcggatatga agtccataaa tcgcacaatt
     5761 cgcgcctgca atttccaagc cgtcaacagg agtagcgctg ccgtttccga ccagcacaat
     5821 cgctcccgga aagtagcccg gttgagtact gccgtctatg acggtacctg cgccggtgat
     5881 ggtgggcagg ctggtaatgg gtgcaatgac atgcgggccg gggccggtaa tattgaaaac
     5941 gatggtgtcg gggccggggt tttggttggc tgctctgatg gcctcccgca gactgcaatg
     6001 ggttgcattg cagacgccgt cgttggtgtc gtctgtggta nnnnnnnnnn nnntctcaac
     6061 tgcgccgcag ccgttgtcag ggtcaacaga cctgtgaaaa gcgtagcaat gaagtgtaaa
     6121 atgtgtgctc tgttcataca atgactctat tctttctgag tgcgattcag acgcagcaag
     6181 aagctgtgag ttgcaaaacc cagcgcaaaa atacagctat ctgatgggag gaaagaataa
     6241 tacttgttgc gcaggccgcg ttgataacgc cgggccgcaa aaattcatac ttttgcggcg
     6301 gaaaaagcaa acttttccgg ctaagtcagg agctcgcttc aaagttggtt cctgacttgc
     6361 caagacgatt tatcacaact ctgtttcatg tcggtatccg tacagcaatt gacaaaaatt
     6421 tacggcgagc agcacgccgt acacgaggtg agttttgagg cgcacagcgg ccaggtactc
     6481 ggttttctgg ggccgaacgg cgcgggcaaa tccaccacca tgaagatcat tacgggcttc
     6541 ctgccgccca gttcgggtac ggccagcgta tgcggctaca acgtggaaac cgccccaatg
     6601 gaggcccgcc gccgcatagg ctacctgcct gaaaacaacc ccctgtacaa agaaatgtat
     6661 gtgcgggaat acctccactt caccggcaag ctgcacaaag tgccccggct gaagcagcgc
     6721 gtagaggaaa tgatagaggc tacgggctta ggccgtgagt gtcacaagct catcggcgcc
     6781 ctctcgaaag gctaccggca gcgcgtagga ctggcgcagg ccatgctgca cgaccccgaa
     6841 gtgctcattt tagacgaacc gacttcgggc ttagatccca accaattggt agaaatcaga
     6901 gcgctgatac gccaattggg ccaacaaaaa acggtcattt tttctacgca catcatgcag
     6961 gaggtgcaag cggtgtgcga ccgcgtcatc atcatcaatc gggggaagat agtggccgac
     7021 gacccgatag aacgcctgca ggagcgcatc gccggagaat cggtgctgac cattgagttc
     7081 cggcaggcgg tgaaaaagga agccctgctg gccattcccg gtgtgaaaca ggccaaaaat
     7141 acaggcggca accgttggca actgagcgct gccgccgatg cagacgttcg cccgcaggtg
     7201 gccgattttg taaagcgcca cgagttggac atcattgaga tgcaccgcga ggccatcagt
     7261 gtggaagagg tgtttaaaaa actgaccagc catgcttaaa tattatttca ttgtcttcca
     7321 gttgtttgca gtttgtctca acgcccaaac cgacagcgta ttggtgacga aaaacttcaa
     7381 gttcaacgac ggggtgtatc tcagttttgc cgactggcgc aacaaccggc ccaactggac
     7441 ctgggacagc ctcgacgtgg tgtcggctac caatccgaac accttcctta cgcaggtaga
     7501 atccattcgt tttgccgatg ggcgcccgat gaatttggac agcatttggg gtttggtcat
     7561 cagcggtatt ccgtatgtgc gtttgccgaa aggcgcggtg cccaaaacgc tcacttcctt
     7621 tgccgggctg cgggtgcgcg ggcgcattgc gtactacaac tacgaggtga ccgaggagga
     7681 aatggtggaa atacgcgcct acaatccggt gacgggcgtg ccgttcagga agggcaaggt
     7741 gaagaagcag tcgaaggcgc tgcgggagtt tatgattcat tttgagcgtg gcgaaacggc
     7801 ggcctttact cgcgaaaacc tgatggcctg gatcgccgac gacgaacgtt tggtagaagc
     7861 cgtggcggcc ctgccggagg nnnnnnnnnn agatttggaa gaaaaattgt tcaaagcatt
     7921 gctgatctat gtggatcggt atcctgttta cactcaaaaa taagacccga tatgtggagc
     7981 attttcgtga aagaaattgc aggttttttc agttcgctca tcggttatgt ggtcgtgggg
     8041 ctgtttttgt tgtttctggg cttggggctt tttgtgtggc ccaacagcag cattttagac
     8101 aacaactacg ccacgctgaa tgagtttttt caaaccgtgc ctactgtgtt tgcctttttg
     8161 attccggcca ttacgatgcg ctctttggcg gaggaaaacc aaagcggcac catagaactg
     8221 ctggctaccc gccccattcg cgatggagaa attgtgctgg gcaagttttt ggccgcgctg
     8281 gcgctcacct gtattgccct gctgcccacg ctcttgtact actttacggt gtatcagttg
     8341 ggcgacccca aaggcaatct ggacgctggc gcggtggcgg gttcgtacat cgggctgttg
     8401 ctgctggcgg cggcctttac ggcggtggga ctgctggcct cggcgctgaa caacaaccaa
     8461 atcgtggcat ttctcatggc ggtattgttg tgcggactct tgttttgggg gttcgatctc
     8521 atcagcagcc tgcctgtgtt tgccggccgg ggcgacgacg tagtgcagca aatcggcatg
     8581 agcgcgcact atgcgtccat cagtcagggc gtgatagaca gccgcgatgt ggtttatttt
     8641 tcatccatca tccttttgtt tctggcgttt acggcagcgg ctgtgaacag cagaaagtgg
     8701 tagtttagtg aaggggtgac tacctggtat tgcctcgcaa gtcacccctt cactaaaccc
     8761 taaacaattc atgctatgtc caagatattt cgcaataaag ttcaaacgct cctgcaactc
     8821 ggcctcctca ccgtagttgt catcttagtc aatattttgg ccaatgcccg cttcggcgat
     8881 aaggccatgt acggacacat agacctcaca gaggagaagc gcttcaccct cacatcggnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgaaa
     9061 acagcctcgt ggagtacgag tttgaagacc ccgccgccgg caccaacgac gaggtcagcg
     9121 atcgccgcaa gcaattggcc gaagatggca tccgccccat caatctgcgc ctcaaagacc
     9181 gacagggcac ttccgaaagg ctcatttacc cgtatgccgt tttttattac aaagggcgca
     9241 acattgtggt gaatctgctg gaaaacgagg taccgggcgt gccgccggaa gtcattctca
     9301 acaatgcggt ggagctgttg gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacccttg gaaaccgccg
     9421 atctggaaaa atcgctgcgg ccttattacg ataccggtcg cctcaacttg gacagcatgt
     9481 cgtccattcc ccaggaggtg gcagctttgg tgatcgccaa gccgcgctat ccgttttctg
     9541 aaaaaagcaa gttcaaactc gatcagtacg tgatgaacgg cggcaaggtg ctttggctca
     9601 ttgacgccct gaggattgac atggacagcc tgcgcacccg cgagcgctat ttggccactg
     9661 aatatacgct caacttagac gacctgctct ttcaatacgg cgcccgcctg aaaacgaatc
     9721 tggtactcga ttatcggtcc acaggcatac agttggtaac cggcatcgtg ggcaatgcgc
     9781 cgcaattcga ttatttcaaa tatccttatc atgtggtggc gctgcccagt gtcaaccatc
     9841 ctattgtgaa gagcctcgac ggcgtgaacc tgcaatatgt gggcgccata gataccatca
     9901 aaaccaaaac gccggtgaaa aaaacggtac tgttggagtc ctcgcagcgc agtatgttgc
     9961 agtattcgcc cgtgacgatg gattttgagt ttttgcgtta cgatctggat tccagcaagt
    10021 tcaacaaaca aaacctgccc atggcgctgc tgctggaagg tacattctct tcgctgtacg
    10081 aaaaccgcat ggagcaggac atgctgaata tgttgcagca gatcggcaag ccataccgcg
    10141 ccgagagcct tccgacccgc atgatcgtgg tagccgacgg cgacgtggcc aaaaacaaaa
    10201 tcaacatcga gaagcagtct tacagcccgc tgggatacaa tgaggtggac cgctatctgt
    10261 ttgccaataa aggattcgtt atcaattcat tggaatacct cttggacgag cagggcgtgg
    10321 ccgaggcgcg cggccgtgag gtcaaactcc gcatgttgga cacggtgcag gcgcaggagg
    10381 aaaaaacgaa atggcgtctg atcaacattg cgttgccttt ggcgctgctg gcgctgttcg
    10441 ggtgggtgtt tcagtggtgg aggaagcgga ggtggagctg agtcggctgg cgggaacggc
    10501 tacaggctgc aagctacaag ccgtatagga agcgtataaa aagcgctatg aagctgtagc
    10561 gcagggttca ttcatcacat tagaagattt gaaaaaagaa tctcagacat ggctaaaagg
    10621 cacccaggct actcagtagt aatctgaagg aagtccgggt aatagacatt atgagaagcg
    10681 gcagaggccc tgaatacatg aaaaaactta aaaaccgcat gtaaaatgaa tcctaaaata
    10741 actttaaacc ctgttgttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt cgacattaac
    10801 tataggttta atggccatca taagtttggg cctgtatgcc cgaacggagc aagacttatt
    10861 catctatgtc ggcggaggat tatttttgtt atccatgttt atagcccact ctcatgtaaa
    10921 gaagaacctc tgagtttgcc gagggcaccc cttcaccgct tcttcaccaa catcctcagc
    10981 caaaacagcg gattggccaa tttcaggcgc agttcaaaat cggtgtacat ttttgataaa
    11041 gactgaatga gcggggcatt gcccgtgatg atgttggcca gcagggtagc aactaccggg
    11101 taggtcaaca tccgttgcag tttgtaactc agcttcatct ccgagccgag cacccgcgcc
    11161 acccgcacat cgtaggcttt cataaattcg gcagaaaagt cgttggcttc caagcatttc
    11221 tgtgccaact gcccggcgat gatgccgctg taaaacgcat tgccgatgcc ctcgccggtg
    11281 aagggatcaa tcaaatgccc cgcatcgccc agcaacatgt acccatcgcc ggaaaggcgc
    11341 cgctctttcg accccagcgg cagcccgtaa ccttctatgt ctccttctat tgcagcatgt
    11401 tcaaaccgcc cccttatgtc cggctcttcc tcaatcagcc gcagcaattc cttgcgcaag
    11461 ttgatgcgct tccggcttac aatatcgctg cgaatgccca gtcccacgtt cgcgcctccg
    11521 tcgggcagtg ggaaaatcca aaggtagccc ggcaacacag atttcaagaa atgcagttcg
    11581 atgaaattgc cctcccggaa gcccgtcacg ccccggtagt aggccctcac ggcggcggca
    11641 tggtgggccg ggtctttttc caaacccgcg tatttgcgac tgaaggcaga gtgcgcgccg
    11701 ttggccgcca gcaacaaacg gcaatcaatg cgcaggcttc cccccgcgtc cgaaagggtg
    11761 aaaccgctgt ccgttttttc aaaatgagtg atttccgtac cttccaaaaa aacgatgttg
    11821 tcgcggcgtt tcaactcttc gatcaaaaac tgatcaaaat ccatcctccg gcacacatag
    11881 cccggcgaag tggcttggtc gttgcgctcg ctgttgtaaa aagggatgtc caaaattttt
    11941 cggttcggcc ccacaaaacg gatgccccag actcctacat gccggttgct cagcgcatca
    12001 aaccgggcca gaatatcggg atcgaggcgg cgcaacaagg tgggaatttt gccgctgatg
    12061 gcatccccgc agattttgtc tcgcggaaag cgcgctttgt cggccacgat acacgggatt
    12121 cctaagtaac tgagttgcaa ggccgccgcc acgcctccgg ggccggcccc gacgatgcag
    12181 atgtcggttt ggtatgtgtt cattcgattt ttgcccccat gaaaaataac ggagatgcca
    12241 caaaaatact acttttgaca accctaacag gccgtttttc attcttacaa taatgatcag
    12301 atcactacta ccggcagcct tcttttggat gatgtttaca gccgccacct gtgagcgccc
    12361 tgttgatttg gaaatactcc tgcccgaacc tgaattggtc atcatcagca cgttttcacc
    12421 gcagcaaccc ttccgcgcca ccgtcagcag tactcgcccg gctttgcccc ccacgccgac
    12481 tacctacatc gccaatgccc gggtagaact tttccaaagc gacaactttc ttcaaaatct
    12541 acagcccgct cccggcactc tggcgcctta ttacgaagcc ttggaagccg taccttcgga
    12601 ggaacaa
//
LOCUS       DAORLF010000078        12533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2677_length_12533_cov_2.144655, whole genome shotgun sequence.
ACCESSION   DAORLF010000078 DAORLF010000000
VERSION     DAORLF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_12533_cov_2.144655"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK198_08465"
     CDS             <1..265
                     /locus_tag="PK198_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38808.1"
                     /translation="YFLIITPPGRREYITNHCDNIANNINIANLRNQFRADIVVVFTN
                     QNYVAGDRRIFGAVNNIGLNPASAFTIVEAPFISALGSPWRTK"
     gene            302..1177
                     /locus_tag="PK198_08470"
     CDS             302..1177
                     /locus_tag="PK198_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38809.1"
                     /translation="MEVMIMKQNCAHGWRFIDGNGIERRTIMALSGAIDINNPTQDVR
                     QRSPLFSNPNIEIQGVAAGTLNDNNAQVMRNNGCFVSQYRTQPEWSAYLIAPDIVCRN
                     QLFFQACANVIPPGTGFSGIGPYLYEIYLSIDFQQEQLMSNLSCVNINQIEGGFLYLR
                     IVVRSADNRVITLSKVIEVRDGTSPPCFIPLRDANSSAAQASGYKHSLAMETGLFPNP
                     TSGIIRLLFNTDAEYYAPVSILVFDQCGKLLKQERITPTHTQHDLDLIQYSSGVYFVR
                     ISWDDDSVTHKIIKL"
     gene            1174..1719
                     /locus_tag="PK198_08475"
     CDS             1174..1719
                     /locus_tag="PK198_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38810.1"
                     /translation="MRLTIINILILFIIVTGCRKEEVLEMPVYFAGDISKGWVDMQKN
                     GIEMKASASSIPCFRDSEQITISLSTVDEFDVLSEYFVLSCFPLTIGKYILCSRNSIS
                     CDYVANYLTIIEDQPEDHYLLNERKPNFLQITDIDMDTRWIGGVVNMHFNHDSSRKPK
                     ENLANPDKVHFYGTFRVKLPD"
     gene            1906..>2963
                     /locus_tag="PK198_08480"
     CDS             1906..>2963
                     /locus_tag="PK198_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38811.1"
                     /translation="MRQILPWFAVVFCLSTGILQAQVLDSLFGVPYSFTLVHFTPGAT
                     SSDFINRDDRCYAALHLPGGKIILAGHTSSADGTDMALLHLLSDGTYDEGVGPGGRIR
                     TNFGHYADSCVAAVLDAEGRILTAGATHEQGQIMFNILVARTDTLGRPDSLFGNNGSV
                     VIDLPPTREIITKIIPLPDGRILIAGDAYYGDDYYFPDSTRILVGRLWPDGSVDTSFG
                     NNGFFYLQFDSGCNSSMLGDIAVDSEGRVLVSGASYFIYPLYFFPTTTCWHVVDLFRY
                     TSGGLPDNSFGTGGKLTLPHTQGHASVLHIGNNDEIVPAGSVTNFEDLHTPVHIYLGR
                     VLAGGTMDSTFAYYGRFI"
     assembly_gap    2964..2987
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2988..3584
                     /locus_tag="PK198_08485"
     CDS             <2988..3584
                     /locus_tag="PK198_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38812.1"
                     /translation="NGGIGPAAPSDLLELADHYYMGFEDDAQGTGLYFGLLRFSKTGA
                     WDMSFGNNGVFNTEGLLPYIGGEVTQISTVDSNYIFISGYHQTTALQKNNMFIAKVKL
                     NNVVSAAEAVKEESKLRIFPNPVSGGVLNVDYSGFADEGLVQLRLSDMQGRLLWQRQV
                     PAPGGIGQADVSQLPPGMYVLEVSNGVQRAVRRVVVAR"
     gene            3648..4985
                     /locus_tag="PK198_08490"
     CDS             3648..4985
                     /locus_tag="PK198_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF38813.1"
                     /translation="MSASILIYADFDSPRLRYALDLIFVVTGQTAYRLTTDWSSYRAC
                     AGPRMAYSQKLIEPDELRIPPCGLLQETSIRPIQADVQERQGLPAFFFCADTQNADTD
                     FDLLALVFFLCSRYEEYLPGPRDAHGRFPAQQSWAQQHDFLHQPLAQQWALHLLERLA
                     QRFPAFRFERPAFRFQPTFDVDMAWAYRYKSPWRQAAAVLRDVLAGDRQALKLRQDVL
                     SNKVEDPFFTFADIEEWHRDPASDPLFFFHLGDYGRFDKNISHRHPMLRTLVQRLSAA
                     HHTGLHPSWKAANDHTVLEKEARRYEEITGHRPTRSRQHFLRMELPRTYQELLDAGIR
                     EDYTMGYAEAPGFRAGMALPFPWYDLEAERCTELMLHPFTVMDVTLKEYQQLSPEEGL
                     HSALRLLETVRETGGVFCTLWHNSSFSDIGQWKPWRAVYREIIAEAARNGNHI"
     gene            5062..7041
                     /locus_tag="PK198_08495"
     CDS             5062..7041
                     /locus_tag="PK198_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF38814.1"
                     /translation="MNRLRLIFTLGWQLAAITLSAQCLSGNCANGSGVYEFPGGSRYT
                     GQFKNWKPTGVGSCAFSDGRKYQGEWLDGYPHGNGLMIYDDGSRKRGIWSKGVLVRET
                     EVRGAQGEFRPRSQPVGCVSGDCQNGDGIYIFPSGAVYIGQFQNGEVHGVGVCDYSDG
                     SRYQGEWKNRFPDGTGTKTYPDGSRRTGRWLQGQPVDNQGNWSSALANTQSDTADPTD
                     IQSGCISGNCQDGTGVMAYPDGSRYDGSFVAGKPHGQGVFRYANHSNYSGQLAAGLPH
                     GRGELQRPDGATLSGKWENGEFIGPDEVRLPQRAECLEGDCRTGTGVYVFKNGARYSG
                     SFLNYMPHGTGTIWYPNGEKYTGDVVEGALHGRGTLYLSDGSPVSGYWQNGVWLGEQA
                     PAVETPPIQVPAPTPSVTQAPTAGKTARPKVWALVVGVASYNHMPTLRYTDDDAYRLY
                     AFLKSPGGGAVPDEQLRILIDESATRDQILAAMETLFSNAGPNDMVVLYFSGHGLKGS
                     FLPFDFDGFGNKLKHEEITEALRNSKARYKLCIVDACHSGSLLSMRGAAQQQQLISTF
                     YEQLAQAEGGTALFLSSKSEETSLESSGLRQGVFSHFLIRGLKGEADADNDRLVTIQE
                     LYEYVYTNVRSYTGMLQSPMITGDYDRKMVLAVKP"
     gene            complement(7051..8127)
                     /locus_tag="PK198_08500"
     CDS             complement(7051..8127)
                     /locus_tag="PK198_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38815.1"
                     /translation="MNVFLKISIQTILLSWLGCTPVAETGPAPVISEADHTTPTFRGK
                     VMQSADMGQTWTDISEGLPEDARPSAVSIADNEAYLGYEKDRLFSLNGAPVQSWKKEQ
                     WGAALSQHTAKADNLILGIYSTKPALYVFVPNDGLYRMQRNARFWAAVKMPVGVFGTN
                     DVEEDESGRIYLADQTGVYVSSDGGQNWDLRMRAGWINSLEMSDNAVLAGGKNGVYRS
                     VDGGTTWAALPIHQQTNFMFAKEGPPIYKVMRSGNVLTATRSEGPSEKGAPGKFQIST
                     DGGLNWQIHPADNFLKNLEDIGSILLHEGKIFCSYKGFVICSEDNGNTWKTLLKSENT
                     EPGAVLVLYISGGVLYCTEGISGC"
     gene            8269..9120
                     /locus_tag="PK198_08505"
     CDS             8269..9120
                     /locus_tag="PK198_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRF38816.1"
                     /translation="MKYIVLLSFCSLVLSILAAACSSDAHDEKYRQVVMRDIGNKILW
                     SLGDSVSRVMPVASKGGAYTISFERPASVSYDSVVAVVSQELIKNGITRFVAEMKDCR
                     TNEVLLAFAFRSPSDSLTPCTGRHTEPGCFTIEITPGREHRTLRAALPFIISGAVGGL
                     LFLYFKSRSGRKHKTAPAKGQGLPVGKYTFDPTEKTLTIDDLILPLTDKEAKLLSLLL
                     SNMNQTLSRETLMAEIWGEDGLMVIARNIDVLVSKLRKKLMHDDSLAITNVHGVGYKL
                     VDVTAKG"
     assembly_gap    9275..9299
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9300..>10079)
                     /locus_tag="PK198_08510"
     CDS             complement(<9300..>10079)
                     /locus_tag="PK198_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRF38817.1"
                     /translation="ILDVRKMLSIQAHPTIEKAREGFRRENEQGIPFTAAHRVYKDPN
                     HKPEVMAALSDFWLLHGFKSPQDIESELARVPELRPLLEHFSHRDTAALYRHLMLMPQ
                     SEVDALLLPLRERLEPLFVAGELPKSSPDYWAALAFRDFELPGGHCDRGIFSIYLLHL
                     AHIPQGQGIFQAAGVPHAYLEGQNVELMANSDNVFRGGLTPKYIAVEELLDNLVFEPT
                     PPRLIEGSSLNEVETVYPTPAPDFELSRLELPAGTDYSFTAG"
     assembly_gap    10080..10123
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10124..10337)
                     /locus_tag="PK198_08515"
     CDS             complement(<10124..10337)
                     /locus_tag="PK198_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38818.1"
                     /translation="MIYRLLPRLQHYDWGGFRFLPELLGQSAPSERPCAELWMGAHPG
                     APAAILLDDREAPLPELIAADPQRWLG"
     gene            complement(10498..>11725)
                     /locus_tag="PK198_08520"
     CDS             complement(10498..>11725)
                     /locus_tag="PK198_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38819.1"
                     /translation="ATAAKLTAAWLTQKTFRFSSDERRLIFGLSNAQAAATLAAVLVG
                     YNIILGQTDTGEPIRLLSESVLNGTILMILVTCTHASFVAQKGAQNIALAETADDDTG
                     SSSYNNEERILISLHNPDTIDELIQLSVTLKSKRSNSHLYALNIIADDNPSASADKKA
                     RQLLQKAAVSAAATDNHLRELLRYDANIPNGISYAVKEHQVTDIIMGLHQKAGLSDSF
                     LGHLTEGILARCNTTTVIYKCAQPLATVRRHFIMVPEHAEHEIGFLFWLIRVWNLGRN
                     TGAKLVFLASDATLRYLTEVHARHPVEAEFRELEDWDDFLDHQNELKTDDGLIIVMSR
                     KGHRSYNTDMARVPEYLNRSYQRNNFILMYPMQAGVDESHSPILKNLSVLEPNAGKPD
                     VIEEFVKSVSRLFQKK"
     assembly_gap    11726..11945
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11946..>12533)
                     /locus_tag="PK198_08525"
     CDS             complement(<11946..>12533)
                     /locus_tag="PK198_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRF38820.1"
                     /translation="AGAIIGPNGLNLMLRDSSIVLFGTVGLLYIMFLAGLEIDLAEFK
                     KNSFKSFTFGMYTFLIPMSIGTAVGLYVLNFSMLSSVLLASMFASHTLIAYPIISKLG
                     VSKNRAVNVAIGGTMITDTLALLVLAIIVGMTQGEVNAAFWTRLSVSVLLFGLAVMFL
                     FPILGRWFFKRYEDSISQYIFVLGMVFLSAFLAEAA"
BASE COUNT         2863 a         3152 c         3358 g         2847 t
ORIGIN      
        1 ttattttcta attattacgc cgcctgggcg gcgtgagtat atcacgaacc attgtgataa
       61 tatagctaat aatatcaata ttgctaatct tagaaatcaa tttcgtgcag atattgtagt
      121 cgtttttaca aatcagaatt atgtcgctgg ggatagacga atttttggtg ccgtaaataa
      181 cattggcctt aatcccgcct cagcttttac catagttgag gcgccattta ttagtgccct
      241 aggttcacct tggcgcacga aatagctcat ttattcggcg cacgccacaa caggtctgca
      301 aatggaggta atgataatga agcaaaattg cgcgcatggt tggcgtttca ttgatggaaa
      361 tgggatagag cgcagaacaa taatggcttt atcaggagca atagatatca ataaccccac
      421 gcaagatgtg cggcagagaa gccctctgtt ttcaaatcct aatatcgaga tacaaggtgt
      481 tgcagcggga acgctaaacg acaacaatgc ccaagtgatg agaaacaatg ggtgttttgt
      541 ttcgcagtac aggacacaac cggaatggag cgcatattta attgccccgg atattgtttg
      601 tcggaatcag ctattttttc aggcgtgtgc caatgtaatt cctcccggaa caggcttttc
      661 tggtattggg ccgtatctgt atgagatata tctttctatt gattttcaac aggagcagtt
      721 gatgagtaac ttatcatgcg ttaatatcaa tcaaatagaa ggaggctttt tatacttgag
      781 gatagttgtc agatcggcag ataatcgggt gataacatta tccaaagtga tagaagtcag
      841 agacggaact tcaccaccgt gctttatccc gttgcgtgac gccaactcgt ctgctgctca
      901 ggcaagcggc tataagcatt ctttggcaat ggagacaggt ctgtttccca atccgacatc
      961 aggaattatt agacttttat ttaacacaga cgctgaatat tatgctcctg tttctatact
     1021 tgttttcgat cagtgcggca aactactaaa gcaagaaaga attactccaa cgcatactca
     1081 gcatgatctg gatttaatcc aatattcatc cggcgtatat ttcgtaagaa tttcatggga
     1141 tgatgattca gtcactcata aaatcatcaa gctatgagac tcacgataat caatatcctc
     1201 atccttttca ttattgttac aggatgtaga aaagaggaag tccttgaaat gcctgtttat
     1261 tttgccggag atattagtaa agggtgggtt gatatgcaaa aaaatgggat tgaaatgaag
     1321 gcgtcggcgt caagtatacc atgctttaga gactcagaac agataacgat ctctttatca
     1381 actgtggacg aattcgatgt tctctctgag tattttgtac ttagttgttt cccattaaca
     1441 attggcaaat atattttgtg ttcaagaaat tcaatttctt gtgattatgt ggcaaattac
     1501 ttaacgatca ttgaagatca acctgaagat cattatttac ttaatgaaag gaagcccaat
     1561 tttttacaga taaccgacat agatatggat acccgatgga taggaggcgt agtgaatatg
     1621 cattttaatc acgatagcag ccggaagcca aaggaaaatc tggccaatcc agataaagta
     1681 cacttctatg gtacgttccg ggtgaagttg ccggattgat gacgactacc aactctgtgc
     1741 tgccgaaaat agcccgcaaa acgcggctac ttcggcagca cctacgaagt aagcctcgac
     1801 gggaagttca ttgcagggca tagaattgtc aaaattcaat aaacatcgtt tcacaagcag
     1861 gctatggcgc gttccggcca tagcctgctc ttaaagctat aagacatgag acagatattg
     1921 ccatggtttg ctgttgtatt ttgcctgtca acaggtattc tgcaagctca ggttttggac
     1981 tctcttttcg gcgttccata ttctttcacc ttagttcatt tcactcccgg agccacctcc
     2041 tctgacttta ttaaccgaga cgaccgctgc tatgccgcac tgcacctgcc gggcgggaaa
     2101 attattctgg ccgggcatac cagcagcgcc gacggcacag atatggcact gctgcacctg
     2161 ctgtctgacg gcacatacga tgagggtgtt gggccgggcg ggcgcatcag aaccaatttt
     2221 gggcactatg ccgacagttg tgtagccgcc gttctggatg cagagggccg gattctgacg
     2281 gcgggcgcaa ctcatgaaca gggacaaatt atgttcaaca tattggtagc ccgcaccgat
     2341 acgctgggcc ggccggactc gcttttcggc aacaacggca gtgtggtcat agacctgccc
     2401 cccacgcggg aaattattac caaaatcatt ccactgcccg atgggcgcat actcatagcc
     2461 ggcgatgctt actatggcga tgattattac ttccccgatt ctacccgaat cctcgtcggc
     2521 aggctttggc ccgacggcag cgtggatacc tctttcggca acaacggatt cttttacctc
     2581 caattcgatt ccggctgcaa ctcctccatg ttgggagaca tagcagtgga cagtgagggg
     2641 cgcgtactcg tttcgggcgc ttcttacttt atatatcctt tgtatttttt tcccacaaca
     2701 acatgctggc atgtcgttga cctgtttcga tatacctccg ggggcctgcc cgacaacagt
     2761 ttcggtacgg gcggcaagtt gacgctccct cacacacaag gccacgcaag tgtattgcat
     2821 ataggaaaca atgatgaaat agtgccggcg ggcagcgtga ccaatttcga agatttgcat
     2881 actccggttc atatttatct tggtagggtg cttgccgggg gaacgatgga ctctacattc
     2941 gcgtactacg gccgctttat cagnnnnnnn nnnnnnnnnn nnnnnnnaat ggagggattg
     3001 gccccgccgc acccagtgat ttgctggagc tggcagatca ctactacatg ggctttgagg
     3061 atgatgctca gggcaccggt ttgtactttg gcctgctccg ctttagcaag acgggtgcat
     3121 gggatatgtc ttttggcaac aacggtgtgt ttaatacaga gggtttactc ccctacattg
     3181 gcggtgaagt aacacaaatt agtactgttg actccaacta tatttttata tccggttatc
     3241 atcagacaac agcactacaa aagaataata tgttcatagc caaggtaaag ctgaacaatg
     3301 tggtatctgc tgcggaggca gtgaaagagg aatcgaagtt gcgcattttc cccaatcccg
     3361 tatcgggcgg cgtactgaat gtggactact ccggtttcgc ggacgaaggg cttgttcagt
     3421 tgcgcctgag cgatatgcag gggcggctac tgtggcagcg gcaggttccg gctccggggg
     3481 gcatcgggca ggcagatgta tcacagcttc ctcccggcat gtatgtgctg gaagtgagca
     3541 acggcgtgca acgtgcagtg cggcgggtgg tggtggcgcg gtagcatcag atagaagatg
     3601 gtggaaaatg caagaggcct ccgtattctt gtgtaatttt gtctgccatg tctgcctcca
     3661 tcctcatcta cgccgacttc gactcgccgc gactgcgcta cgcgctcgat ctgattttcg
     3721 ttgtgacagg tcagacagcg taccggctca cgaccgattg gtcgagctac cgcgcctgcg
     3781 ccggcccgcg catggcatat tcccaaaaac taattgaacc agacgaactg cgcataccgc
     3841 cctgcggcct gcttcaggaa accagcatcc gcccgataca ggccgatgtg caggaacgcc
     3901 aggggctgcc ggccttcttt ttttgtgccg acacccaaaa tgcggataca gatttcgacc
     3961 ttttagcgct cgtttttttc ctttgttcca gatatgagga gtacctgccg ggaccaagag
     4021 acgcgcacgg caggtttccc gcgcagcaga gttgggcgca gcaacatgac tttttgcacc
     4081 aacccttagc tcaacaatgg gcattgcact tgctcgaacg attggcgcag cgctttccgg
     4141 cattccgttt cgagcggccg gcatttcgtt ttcagcctac tttcgatgtg gacatggcct
     4201 gggcttaccg ctacaaatcg ccctggcggc aggcggcggc ggtgctgcgt gatgtactcg
     4261 ccggcgacag gcaggctttg aagctacgac aggacgtgtt gtcgaacaaa gtggaagacc
     4321 cgttttttac tttcgccgac atagaagagt ggcaccgcga cccggcctcc gatccgctgt
     4381 ttttctttca tctgggcgat tacggccgtt ttgacaaaaa tatttctcac cgtcatccca
     4441 tgttgcgcac actcgtgcag cggctctcgg cggcgcatca cacgggcttg catccttcct
     4501 ggaaagcggc aaacgatcac actgttttag agaaagaagc ccggcgatac gaggaaatta
     4561 ccgggcatcg ccccacgcgc agccggcagc attttttgcg catggaactg ccgcgcactt
     4621 accaggaact gctcgatgcg ggtattcggg aagactatac gatgggctac gccgaggcgc
     4681 cggggtttcg ggcgggtatg gctttgcctt ttccctggta cgatctggag gccgagcgct
     4741 gcaccgaact gatgctgcat cctttcaccg tgatggacgt gacactcaaa gagtaccagc
     4801 aactcagccc ggaggaaggg ctgcacagcg cactccggct gctcgaaacc gtgcgcgaaa
     4861 ccggcggcgt cttctgcacc ctgtggcaca acagttcttt ctccgacatc ggccaatgga
     4921 agccctggcg ggcagtatat cgcgagatca tagcggaggc ggctcgaaac ggcaatcata
     4981 tttgatttta tcacatcttt tttgcgcttg aactcatctt catacgagct ttcagcgttt
     5041 gacattacca aaaccgatag catgaatagg ctccgactta tcttcacact cggctggcag
     5101 ttggccgcca tcaccttatc tgcacaatgt ttgtcgggca attgcgccaa tggcagcgga
     5161 gtttatgaat tccccggcgg ctcgcgctac accggccagt tcaaaaactg gaagccgacg
     5221 ggcgtgggaa gctgcgcttt ttccgacggg cgaaaatacc agggcgagtg gttggacggc
     5281 tacccgcacg gcaacgggct gatgatctac gacgacggca gccggaaacg aggcatctgg
     5341 agcaaaggcg tgttggtgcg cgaaacggag gtgcgcggcg ctcagggcga gtttaggcca
     5401 cgctctcagc ccgtgggatg cgtttccggc gactgccaaa acggcgacgg catctacatt
     5461 ttccccagcg gcgcggtgta tatcgggcag tttcaaaacg gagaagtaca cggcgtgggc
     5521 gtttgcgatt acagcgacgg cagccggtat cagggcgaat ggaaaaacag attccccgac
     5581 ggcaccggca ccaaaaccta ccccgacggc agccgcagaa ccggacgttg gttgcagggt
     5641 caacccgttg ataatcaggg aaattggtcg agcgctttgg caaatacaca aagcgacacc
     5701 gccgacccca ccgatataca atcgggctgt atttcaggca attgtcagga cggcacgggc
     5761 gtgatggcct atcccgacgg cagccggtac gacggcagtt ttgtagcggg gaaaccgcac
     5821 gggcagggcg ttttccgata tgccaatcac tccaactact ccgggcaatt ggctgcgggg
     5881 ttgccccacg ggcgcggcga actgcaacgc cccgacggcg ccaccctctc cggcaaatgg
     5941 gaaaacggcg agttcatcgg ccccgatgaa gtgaggcttc cgcagcgcgc cgaatgcttg
     6001 gagggcgact gccgaaccgg taccggcgtg tatgtgttca aaaacggcgc ccggtattcc
     6061 ggcagctttc tcaactacat gccgcacgga acgggcacaa tttggtatcc caacggcgaa
     6121 aaatataccg gcgatgtggt cgaaggcgca ctgcacgggc gcggcacttt gtacttgagc
     6181 gacggatcgc ccgtgagcgg ctactggcaa aacggcgtgt ggctcggcga acaggcgccg
     6241 gctgtagaaa ccccgccgat acaagtgccc gcgccaacac cgtcagtaac gcaagcaccc
     6301 acagcgggca aaaccgcccg gcccaaagta tgggcattgg tggtgggcgt ggcttcttac
     6361 aaccacatgc ccaccctgcg ctacaccgac gacgacgcct accggctcta cgctttcctc
     6421 aaaagtccgg gcggcggcgc tgtccccgat gagcaactcc gcattttgat tgacgaatcg
     6481 gccacccgcg atcagattct ggcggccatg gaaacgcttt tttccaatgc cggtcccaac
     6541 gacatggtag tgctgtattt ttcagggcac gggctgaaag gctccttcct gccctttgat
     6601 ttcgacgggt tcggcaacaa actgaagcac gaagaaatca ccgaagcgct gcgcaacagc
     6661 aaggctcggt acaaactctg catcgtggat gcctgccatt cgggcagcct gctgagcatg
     6721 cgcggggcag cccagcaaca gcagctcatc agcacttttt atgaacaatt ggcgcaggcc
     6781 gagggcggca cggctttgtt cctgtcgtcc aaatcggagg aaacttcgtt ggaatccagc
     6841 gggttacggc agggcgtatt cagccacttc ctcatacgcg gcctcaaggg cgaagccgat
     6901 gccgacaacg accgtttggt caccattcag gaattgtatg aatacgttta caccaacgtg
     6961 cgcagctata ccggcatgct ccagtcgcct atgattaccg gcgattacga ccgcaaaatg
     7021 gtactggcgg tgaagccgta aagaggaaaa tcaacagccg gaaatgccct cggtgcagta
     7081 caacacgccg ccggaaatat acagcaccag cacggcgccc ggctccgtgt tttcgctctt
     7141 caggagggtt ttccaggtgt tgccgttgtc ttctgaacaa atgacaaaac ccttgtaact
     7201 gcaaaagatt ttgccctcgt gcaggagtat ggaaccgatg tcttccagat ttttgaggaa
     7261 gttgtcggcg ggatgtattt gccagttcag gccgccgtct gtggaaattt gaaacttacc
     7321 gggcgcgcct ttttcagatg gtccctcgct tcgggtggcc gtcaagacgt tgcctgagcg
     7381 cattaccttg taaataggcg ggccttcttt ggcaaacata aaattggttt gttgatgaat
     7441 cggcagcgct gcccaggttg tgccaccgtc aacggaacgg taaaccccgt tttttccgcc
     7501 ggcgagaacg gcattgtcgc tcatttccag gctgttgatc caaccggccc tcatgcgcaa
     7561 atcccaattt tggcccccat cggaggagac ataaacgccg gtttgatctg ccagatagat
     7621 acgtccggac tcgtcttcct ccacgtcatt ggtaccgaag accccgacgg gcattttcac
     7681 cgccgcccag aagcgcgcat tgcgttgcat tctgtagagt ccgtcattcg gcacaaaaac
     7741 gtagagcgcc ggctttgtgg agtaaattcc caaaatgaga ttgtccgctt tggccgtgtg
     7801 ttgagacagg gccgctcccc attgttcttt tttccaggat tgaacaggag cgccattgag
     7861 tgaaaatagg cggtcttttt cataacccag atatgcttcg ttatcggcga tgctcactgc
     7921 ggaagggcgg gcgtcttcgg gcagcccctc gctgatgtct gtccaggttt gacccatgtc
     7981 ggcagattgc atgactttgc cccgaaaagt cggcgtagta tggtcagctt ccgaaatgac
     8041 cggagcgggg ccggtttcag ccacaggggt gcatccgagc caggagagca ggatggtttg
     8101 gatggaaatt ttcaaaaaca cgttcatgat gcaatgattt tagttgggtg aaaaatgccc
     8161 gacaaaacta ccctcaaaaa cacccggcac ctttgatttg cctgtaaaat atgtgtagag
     8221 aagtgtaaat gtggcgtttt acactttttt gcgaggtatc tttgccgcat gaaatacatt
     8281 gtattgctga gtttttgcag cctcgtactt tccattttgg cggcagcctg tagctccgat
     8341 gcgcacgacg aaaaataccg gcaggtggtc atgcgcgaca tcgggaacaa gattctgtgg
     8401 tcgctgggcg attccgtttc ccgcgtgatg cccgttgcat cgaagggcgg cgcctatacc
     8461 atctcgtttg agcggcccgc ttccgtttcg tacgattcag tcgttgctgt tgtatcgcag
     8521 gaattgatca aaaacggcat cacccggttt gtggcagaaa tgaaagactg ccgcaccaat
     8581 gaggtattac tggcttttgc ctttagaagt ccttccgact cgctgacgcc ctgcaccggc
     8641 cgacataccg agccggggtg ttttaccatc gaaataacgc cgggtcgcga gcatcggacg
     8701 cttcgtgccg ccctgccttt tatcatatcg ggagcagtcg gcggactgtt gtttttatac
     8761 ttcaaaagca ggtcgggccg aaaacacaaa accgcgcctg ccaagggaca agggctgcct
     8821 gtcggcaaat acacgtttga tcccacagaa aaaacgctga cgattgacga cctcattttg
     8881 ccgctcacgg acaaagaagc aaagctgctg agcttgctgc tgagcaatat gaaccaaacc
     8941 ctgtcgagag agacgctcat ggccgaaatc tggggcgaag acgggctgat ggtcattgcc
     9001 agaaacattg acgtactggt gtccaaactc cgcaaaaaac tgatgcacga cgacagcctc
     9061 gccattacca atgtgcatgg cgtagggtat aaattggtgg atgtgacggc caaagggtaa
     9121 gggctgtaga agtctatccg ccccaaggca ccgcggcctt gaacagcacc gaatgtgcgg
     9181 ccgcctgcaa gcgacattcc gtttcgggca gcaggtacat gacctcaccc tgatgaaaga
     9241 cctcgccggt atcggcagtc acttcgccct gcatnnnnnn nnnnnnnnnn nnnnnnnnng
     9301 ccggcggtaa acgaatagtc tgtccccgca ggcagttcta agcggctcag ctcgaaatcg
     9361 ggagcgggcg tgggatacac agtttccacc tcgttgaggg agctgccttc gatgagtcgg
     9421 ggcggcgtag gctcaaacac cagattgtcc aacaattcct ccacggcgat gtatttgggc
     9481 gtaagcccgc ctctgaaaac attgtcggaa ttggccatca gttctacatt ttggccttcg
     9541 aggtaggcgt gcggcacccc cgccgcctga aagatgccct gcccctgcgg gatgtgtgcc
     9601 aagtggagca gatagatgga aaaaatgccc cggtcgcaat gcccgcccgg cagttcaaaa
     9661 tcgcggaaag cgagggcggc ccaataatcg ggcgaagatt tgggcagctc gccggccaca
     9721 aacaacggct ccaagcgctc ccgaagcggg agcaacaagg cgtccacttc gctttggggc
     9781 atcagcatca ggtggcgata cagcgcggcg gtgtcgcggt gggaaaagtg ttcgagcaga
     9841 gggcgcaatt cgggtacgcg cgccaattca gactcaatat cctgcggaga tttaaacccg
     9901 tgcaggagcc aaaaatccga gagggccgcc atcacttccg gtttgtgatt ggggtctttg
     9961 taaacccggt gcgccgctgt gaacggaatg ccctgctcgt tttcgcggcg aaagccctcg
    10021 cgcgcctttt cgatggtggg atgcgcctgt atggaaagca ttttgcgcac atccagaatn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacccag ccatcgttgt
    10141 gggtcggcgg cgatgagttc gggcagcggc gcctcccggt cgtcgaggag aatggcggcg
    10201 ggtgcgccgg ggtgagcgcc catccagagt tcggcgcagg ggcgttcgga gggcgcggat
    10261 tggcccagca gttcgggcag gaagcggaat ccgccccaat cgtagtgttg caggcgaggc
    10321 agcaggcgat atatcatatg aaatcaatta tttgtttatc tgatgagcta tgagccgcga
    10381 gctacgagcc gcgagcttgg ttttccaaaa gtgcatttaa aaatctcctc ggatgaaaaa
    10441 tctaattacc tgacgagaaa cgtgcttgtg gcttacagct tgtggcagta acggaagtta
    10501 ttttttctga aacaatcgcg aaacagactt gacaaactcc tcaatcacat ccggtttgcc
    10561 ggcgttaggc tccagcaccg agagattttt caaaatagga gaatggctct cgtccacacc
    10621 ggcctgcata ggatacatca ggatgaaatt gttgcgctga taagacctgt taagatattc
    10681 cggcacgcgg gccatgtctg tgttgtagct gcgatggccc tttcggctca taacgatgat
    10741 gaggccgtcg tcggttttga gttcgttttg gtgatccaaa aagtcgtccc agtcttccaa
    10801 ttctctgaac tccgcctcca ctgggtgacg ggcatgtact tctgttaggt agcggagggt
    10861 ggcgtcggag gccagaaaaa cgagtttggc gccggtgttg cgccccaggt tccacacccg
    10921 gatgagccag aaaagaaacc cgatttcgtg ctcggcgtgt tcgggcacca taatgaaatg
    10981 gcggcgcact gtggccaagg gttgggcgca tttgtagatg accgttgtgg tgttgcaacg
    11041 cgccagaatg ccctcggtga ggtggccgag gaaggagtcc gacaggccgg ctttttggtg
    11101 caggcccatg atgatgtcgg tgacctgatg ctctttgacg gcatacgaaa taccattcgg
    11161 gatattggca tcgtagcgca acagttctcg caggtggttg tcggtggcgg cggcggaaac
    11221 ggcggctttt tggagcagtt gccgtgcctt tttgtccgcg gaagcggagg ggttgtcgtc
    11281 ggctataata ttgagcgcat acaggtggct gttgctcctt ttcgatttga gcgtcacgct
    11341 gagctgaatg agttcgtcaa tggtgtccgg attgtgcagc gaaatgagga tgcgctcttc
    11401 attgttgtag ctgctgctgc cggtatcgtc gtcggcggtt tcggccagcg caatgttttg
    11461 agcgcctttc tgtgccacga aggaggcatg ggtgcaagtg acgaggatca tcagaatggt
    11521 gccgttgagc acgctttcgc tcaataagcg gatgggttcg ccggtgtcgg tctggcccag
    11581 aatgatgttg tatcctacca gcacggcggc caacgtggcg gccgcctgcg cattgctcaa
    11641 accaaagatg agcctgcgtt cgtcggaaga gaatcggaac gttttttggg tcaaccatgc
    11701 tgcggtcaat ttagctgccg tcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnngccgc ttcggccaga aatgcagaca gaaaaaccat gccgagcaca aagatgtatt
    12001 gcgaaatgct gtcttcgtag cgcttgaaaa accaacgccc cagtatgggg aacagaaaca
    12061 tcaccgcgag gccgaacagc aacacggaaa cgctgagcct cgtccaaaat gcagcattca
    12121 cctccccctg cgtcatgcct actataatgg ccagcaccaa cagggccaat gtatcggtaa
    12181 tcatggtgcc gcctatggcc acattgacgg cgcggttttt ggaaacgccc aacttgctga
    12241 tgatggggta tgcaatcaac gtgtgcgagg caaacatact ggccagcaac acagaggaca
    12301 gcatggaaaa attcaacaca tacagcccta ccgccgtgcc gatactcata ggaatgagaa
    12361 aggtgtacat gccaaaggtg aaactcttga agctgttttt tttgaactcg gcgaggtcta
    12421 tttccaaacc ggccaaaaac atgatgtaca acaaacccac cgtaccgaaa aggacgatgc
    12481 tgctgtcgcg caacatgagg ttgaggccgt tgggcccgat gatggcgccg gcg
//
LOCUS       DAORLF010000079        12511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2683_length_12511_cov_2.491811, whole genome shotgun sequence.
ACCESSION   DAORLF010000079 DAORLF010000000
VERSION     DAORLF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_12511_cov_2.491811"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>319)
                     /locus_tag="PK198_08530"
     CDS             complement(<1..>319)
                     /locus_tag="PK198_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRF38821.1"
                     /translation="LITTFNAEYAAALGISVTFWHYLLMSAVSVTTVVSFEVVGAILV
                     VAFLIVPPATAYLLTQSIRPMLILTTLLGFGASLGGYYLAVAINGSIAGAMSVVAGVF
                     FLVA"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<330..815)
                     /locus_tag="PK198_08535"
     CDS             complement(<330..815)
                     /locus_tag="PK198_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRF38822.1"
                     /translation="MDAFWIILVGTLTAVCGGLLGAFLLLRNMSMIGDAISHAVLPGI
                     VIAYLLSGSREALPMLLGAAAVGVLTTVIIEALYRKARLQSDAAIGITFTGMFALGIL
                     LISAFAGQIDLDQDCVLFGEIAYVPIDVWVTSGGLNMGPRAVWILGGLLLLILLLLTV
                     GY"
     gene            complement(815..2107)
                     /locus_tag="PK198_08540"
     CDS             complement(815..2107)
                     /locus_tag="PK198_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRF38823.1"
                     /translation="MSAFWSDPNVQMVVLGVMLLGAGAGVTGVFTFVRKRALVGDAIA
                     HSVLPGVCLAFLISGQKHPLWLLIGAVATGWLSLLTMDYITRNSKIKADAAIGVVLSV
                     FFGLGILLLTAIQASGSGHQAGLDKFLFGKAASLTRQDVLVFGAAAGLILSVSALFFR
                     AFSTMSFDREFAMSAGLPVKTLEFILATITVLAVATGIQAVGVVLMAALLITPAAAAR
                     FWTNNLVKMTLLAAIFGMVSGLFGAWISYQAPAMPTGPWIVILLTLIALFSVLAAPER
                     GILARWQLQRNNAHKMLTENILKTFFQMEELHGKQLFSWEAILQKGHFREQELKKGLG
                     QLRRRGLLAAPDVNGLWRLSSEGETESRRVVRLHRLWELYLSHKLGMAADHVHDSAEA
                     MEHLLTPDMEKRLLEELEYPALDPHDSKIPYDHQPNGK"
     gene            complement(2104..2895)
                     /locus_tag="PK198_08545"
     CDS             complement(2104..2895)
                     /locus_tag="PK198_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRF38824.1"
                     /translation="MSTAIETHNLTVSYHRKPVLWDIDFELPEGQLIGIIGPNGSGKT
                     TLLKSIMGLIPPAGGYVKIFDQPLDEVRSRISYVPQRESVDWDFPVSVRDVVLMGRYR
                     PGRLLRRLSAEDHAIAMESLRKTGMEEFAGRQISQLSGGQQQRVFIARALAQQADLYL
                     MDEPFAGVDAASEQAILQLLADMKSEGKTVVMVHHDLQTVRQYFNWIVLLNTRLIACG
                     PVESVLTEDNLRKAYGGQLTILSNVAHLAQQRDFPMREQAKKQKP"
     gene            complement(2970..>3476)
                     /locus_tag="PK198_08550"
     CDS             complement(2970..>3476)
                     /locus_tag="PK198_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRF38825.1"
                     /translation="LWARAVREVGKQLAQADTANASVYRSHTATYAAQLDSLHRRVLE
                     RMASIPADQRVLVTAHDAFGYFGRAYQIEVHGLQGISTASEYGLRDISDMVNFLTERK
                     IKAVFVESSVPAKSLEAVLEGCARRGHQIRIGGHLYSDAMGEKGSPEGTYIGMVEANV
                     RGIVGGLR"
     assembly_gap    3477..3511
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3512..3959)
                     /locus_tag="PK198_08555"
     CDS             complement(<3512..3959)
                     /locus_tag="PK198_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRF38826.1"
                     /translation="MFLLKITNKMRLSFPSFLRDIQLLVAIPIFLAAVSTACSTDEQQ
                     GAEKLNIVCTTGMIGDALTHIAGDAAQVTALMGPGVDPHLYKPTQGDIGLLSEADIVV
                     YNGLHLEGKMSEVLKKLSRTKKVIALGALLPPEWLRHPEGDSQAHDP"
     gene            4066..4677
                     /locus_tag="PK198_08560"
     CDS             4066..4677
                     /locus_tag="PK198_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38827.1"
                     /translation="MKKTFTLFVFMLLFSFTGWSQAYMDEIAKKCCDCAAKVNPDLKE
                     NEFVMAIGVCMIEVAMPYRKKLKKDFGVDMTRIDLEGEKLGAVIGAHMVAFCPDLLMR
                     LAGEVNDENAVEEQSAVLSAKGIVTRVEKDFLVVFHIKDETGKTIKCYWMAEIDSDLD
                     LVMEYESLSGKKVEVHYVEEKLFDPRISEYRPHFVLTGLYRAD"
     gene            4727..>5913
                     /gene="sucC"
                     /locus_tag="PK198_08565"
     CDS             4727..>5913
                     /gene="sucC"
                     /locus_tag="PK198_08565"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRF38828.1"
                     /translation="MNLHEYQGKELLKKYGVAVQEGILANNVEEAVAAYGQIKARTGS
                     DFALVKAQIHAGGRGKGGGIKLVKNLDDLKDKAGQIIGMMLKTPQTPGGMDGAGKLVR
                     KVLIAEDAYAPDFDACKEYYVSILMDRERKCNVIVYSTEGGMDIEAVAEHTPHLVHKE
                     YIHPTLGLQAFQARKIAFNLGLSGKAFKEMTAFIHSLYAAFLGADASLFEINPCLKTG
                     DDRIIAVDCKVTLDDNALYRHSDLADQRDTDEEDPTEVEAKSYGLNFVNLDGNVGCMV
                     NGAGLAMATMDIIKLSGGDPANFLDVGGTADAKRVENAFRIILRDERVKAILINIFGG
                     IVRCDRVAQGVVDAYRNIGDIKVPIIVRLQGTNADLAKQILDESGLAVRSAIQLQEAA
                     DLV"
     assembly_gap    5914..5977
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6021..8048
                     /locus_tag="PK198_08570"
     CDS             6021..8048
                     /locus_tag="PK198_08570"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF38829.1"
                     /translation="MTNQSVSLRAADNIRILAAAMVEKAKSGHPGGPMGGADFVHVLY
                     SEFLNYDPSDMSWPLRDRFFLDAGHMSAMLYAQQTLLGNYSMDDIRNFRQWGSPTPGH
                     PELDVARGIENTSGPLGLGHAFGIGAALAERFLAARFGEEVAHKTYIYISDGGVQEEI
                     SQSAGRIAGFLGLGNIIMFYDANDIQLSTPVEAVTGEDTAMKYQAWGWHVLTIDGNDH
                     DQIRQALQAGIAETARPTLIIGKTLMGKGTRTADGALFEGEVELHGQPLSGAGASFEK
                     TIAGLGGHPDQPFEIFSDVAEYHQTVLDNKKKAAKKRKSAFKTWAKSNPEQAAKFNFF
                     LSGQLPDIGWSNISLKPNDATRNASGAVLARLAERVENMIVASADLANSDKTENFLKK
                     TKAFAPGDFSGAFLQAGVSELTMAALSIGMSLHGGVIPACATFFAFSDYMKPAIRVAA
                     LMEQPIIFIWTHDAFRVGEDGPTHQPVEQEAQIRLMEQLKNHSGRNSFLALRPADGAE
                     TLESWKLALENRHGPSGLILSRQGIADLPAETTVKAAGTAKGAYIVEDCEGKPDVILL
                     ANGSEVATLIAGAARLRNEKNLKVRIVSVPSEGLFRSQTLKYQRSVLPGNVPVFGLTA
                     GLPVTLQGLAGSRGRVFGLSHFGYSAPFKVLDEKFGFTGDNVFDQVLRLLK"
     gene            8179..9564
                     /locus_tag="PK198_08575"
     CDS             8179..9564
                     /locus_tag="PK198_08575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRF38830.1"
                     /translation="MRKFLIASDSFKDALPAAEVCRAIARGIRRAVPDTDIIELPMGD
                     GGEGTARIITQHVGGQMLDVEAKDPLFRTIHTQYGISSDGNTAYIDMAAASGLQLLRP
                     EERNPLYTTSYGTGEIILDAVKRGARHIVLCIGGSATHDCGAGMAEALGYTFLDAGGQ
                     PVSPTGNNLIKIAYIDAAGLKVNLDWLRVDVLCDVVNPLYGPNGAAHIFARQKGADDK
                     AVELLEEGTKHFSILLQEHLDKKTASISGAGAAGGMGAGAWAFLNAHIIPGTEYVSEV
                     AGLRNAMAEADIVFTGEGRLDEQTINGKLISGIVRAAAEYQIPVIALCGSLGIHPGQM
                     ERLGITAAFAINNRPCTLAEALAETAVNLEHLAFSLACTLAASERYIAVAGMGSGKEL
                     NRRAADILSKRETEVFQLIAKGFTNPQIASTLFISSQTVAVHRKNIMRKLGVNKVTAL
                     LKKGFDSGIFF"
     assembly_gap    9713..9741
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10235..>12511
                     /locus_tag="PK198_08580"
     CDS             10235..>12511
                     /locus_tag="PK198_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF38831.1"
                     /translation="MERTSFTTNRSWILGILTLVLGLAGFQSANAQCPTPTGLTALNI
                     GQNSATLRWTSNSTPTDNCWTVTVGGMGVASCNNAGQATIQTTVCYINNVVSFSAPVT
                     GMTVVGSQINVTVGGLQPGTDYEYYVAETCDGIGAPLNVSACAGPAAFITLDAQYTVT
                     ATTVRPSCPFISPGYVANGSFTVTVTDGTTCAGTYTVNATPVAGSGPAGSTPPSTTVV
                     TYIGFPQGAFLFANAGAGQYTVTVTETGSCNPATDPVVITVTVPDGIDNTAPVYYVTD
                     VLGNILADNDGATAAGTTRNFGTVAVPEGECGRQDEYYVYGFDNCDGFITATNAVSAS
                     AVTVPGTIQPGTQVSTTPDGFGFTLVDVHWSTGTSTVTILGRDAAGNIANGAAGLQLI
                     MNVPDNTDPVVSILGNSQFTIPVCATSVTGILTFQVDDLCDQNNVNFANLIVNFGGAT
                     GVLNFTGNNYREYLVTFPAAGNYLVSASYTDANGNQGFIDQVIQVQLAAANQPATIQA
                     NAETVTLASCQTSAQIVYSFTISDDCEPINIANVQFNGGGSGLPNLNGAGFFFTDPVG
                     PNTVYFEVQGNVGPAGSYFPLITYNGVTANPTITVIQNANQPADIVLPAINVTIPQCA
                     SSVDVVIPITITDDCDNPIVPARASFTLGGVAITPNFVNAAAGYFEFARTLTAANSGQ
                     LLAATYTDAQGAVRTVDALITVNAQPDTWAPIIVYPAQDIIVNLDCCGPSTAIVNFEA
                     TATDNCGIAAFTASFAPGV"
BASE COUNT         2969 a         3493 c         3149 g         2762 t
ORIGIN      
        1 agctaccaga aaaaacaccc ccgccaccac cgacatcgcc cccgcaatgg agccgttgat
       61 ggccacagcc agatagtacc cgcccagcga tgcgccgaac cccaacagtg tagtcaaaat
      121 cagcataggg cgaatgcttt gtgtcagcaa ataggctgta gccggtggca caatcagaaa
      181 cgccaccacc aaaatggcgc ccaccacctc aaacgaaacc accgtagtga ccgacaccgc
      241 gctcatcagc aggtaatgcc agaacgtgac cgaaatgccc agcgccgccg cgtattctgc
      301 attgaaagta gtgatgagcn nnnnnnnnna tatcccacgg tgagcagcag caaaatcagc
      361 agcagcaggc cgcccagaat ccacaccgcc ctcggcccca tgttgagtcc gccggacgta
      421 acccacacat caatcggcac atacgcaatc tcgccgaaga gcacgcaatc ctgatccaga
      481 tcaatttgcc cggcaaaggc cgatatgagc aaaatgccca gtgcaaacat gcccgtaaaa
      541 gtgatgccga tggccgcatc cgattgcagg cgggcttttc ggtacagcgc ttcgatgata
      601 acggtggtca aaacgcccac agccgcagca cccaacaaca tgggcagcgc ttctctggaa
      661 ccggaaagca ggtaggcaat gacaatgccc ggcaacacgg cgtgagaaat ggcatcgccg
      721 atcatagaca tattgcgcag cagcaggaat gcgcccagca aaccgccgca cacggctgtc
      781 agggttccga caagtatgat ccaaaatgcg tccattattt tccgttgggt tggtgatcgt
      841 aagggatttt gctgtcgtgc gggtctaagg ccgggtactc caactcctcc agcagccgct
      901 tttccatgtc gggcgtgagc aggtgttcca tggcctcggc gctatcgtgt acatggtcgg
      961 cggccatgcc caatttatgg ctcagataca actcccacag gcggtgcagg cgaaccaccc
     1021 gccggctttc ggtctcgcct tcagagctga gtcgccaaag gccgttgaca tcgggagccg
     1081 ccaacagccc gcgtcggcgc aattggccca gccctttttt caattcttgt tctctgaaat
     1141 ggcctttttg caggatggct tcccatgaaa aaagctgctt tccatgcagt tcttccattt
     1201 gaaaaaaagt ttttaaaata ttctctgtca gcattttgtg tgcgttgttg cgctgtaatt
     1261 gccaacgggc caaaatgccc cgctccggcg ctgccaacac agagaacaat gctatgaggg
     1321 tgagtaaaat gacgatccag gggccggtag gcatggccgg cgcctgatac gaaatccagg
     1381 ccccgaacaa accggatacc atgccgaaaa tcgccgccaa tagcgtcatt ttgaccaaat
     1441 tattggtcca aaatctggcc gctgccgccg gggtgatgag caatgccgcc atcaatacca
     1501 cgcctactgc ctgaatgccg gtagctactg ccaatacggt gatggtggcc agaataaact
     1561 ctaaagtctt taccggcaaa ccggccgaca tggcaaactc ccggtcgaac gacatcgtgc
     1621 tgaacgccct gaaaaacaag gcgcttacac tcaaaataag gcccgctgcc gccccgaata
     1681 cgagcacgtc ttgccgggtg agcgaggccg ctttgccgaa gaggaatttg tctaagcccg
     1741 cctgatgccc gctgccggaa gcctgtatgg cggtgagcaa aagaatgccc aatccgaaaa
     1801 acacgctgag taccaccccg atagccgcat ccgccttgat cttgctgttg cgggtgatgt
     1861 agtccattgt cagcagcgag agccaacccg tcgccaccgc cccgatgagc agccacagcg
     1921 ggtgtttctg gccgctgatg aggaaggcca gacatacgcc cggcagcacg gaatgtgcta
     1981 tggcatcgcc caccaatgcc cgcttgcgca cgaaggtgaa tacgcccgtg acgcccgcgc
     2041 ctgcgcccag caacatcacg ccgagcacga ccatttgtac gttgggatcg ctccaaaatg
     2101 cgctcatggc ttttgttttt tagcttgttc ccgcatcgga aaatcgcgtt gttgggccag
     2161 atgcgccaca tttgataaaa tagtgagctg ccctccgtag gctttgcgca agttgtcttc
     2221 ggtgagtacc gattcgactg gcccgcaggc aatcaggcgg gtattgagca acacaatcca
     2281 gttgaaatat tgccgcaccg tttgcaaatc gtggtgtacc atgaccaccg ttttgccttc
     2341 ggatttcatg tcggccaaga gctgcaaaat ggcctgttcg gaagcagcgt ccacaccggc
     2401 aaacggctcg tccatcaggt acagatcggc ctgctgtgcc agtgcgcggg cgatgaaaac
     2461 gcgttgttgc tgcccgccgg agagttggga aatctgcctt ccggcaaact cctccatgcc
     2521 ggttttgcgc agcgattcca tggcaatggc atggtcttcg gcgctcagcc ggcgcaacaa
     2581 ccttccgggg cgataccggc ccatgagcac tacgtcgcgc acgctcacgg ggaaatccca
     2641 gtccaccgac tcgcgctgcg gcacatagct gatgcggctt ctcacctcgt ctaagggctg
     2701 gtcgaatatt ttgacatagc cgccggccgg tggtatcagc cccatgatgc ttttgagcag
     2761 ggtggttttg cccgatccgt tgggaccgat gatgccgatg agttgtcctt ccgggagttc
     2821 aaagtctata tcccacaaca ccggcttcct atggtaagat acggtcagat tatgtgtttc
     2881 tattgctgtt gacatgatgc cgtctatttt attcctgatt cctgattcct gattcctgat
     2941 tcctgattcc tgattgcact gcaaaccgcc taccgcaaac cgcctactat tcccctgaca
     3001 ttcgcctcca ccatgccgat ataagtaccc tccggacttc ccttttcgcc catagcatcc
     3061 gagtacaaat gccctccgat gcgaatctga tgtccccgtc gggcgcaacc ctccaataca
     3121 gcctccaaag acttcgcagg cacagagctt tctacaaaaa cagctttgat ttttcgctcc
     3181 gtcaggaagt tcaccatgtc gctgatgtcg cgcagcccgt attccgaggc agtggaaatg
     3241 ccctgcaaac catgcacttc tatctgatac gcccggccaa aatacccgaa ggcatcgtgt
     3301 gccgtcacca atacgcgttg atcggcggga atggaggcca tgcgctccag caccctgcga
     3361 tgcaggctgt ctaattgcgc ggcgtatgtt gccgtatggc tcctgtaaac gcttgcattg
     3421 gctgtgtcgg cttgcgccaa ttgcttaccg acttcccgca cggcccttgc ccaaagnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgggtcgt gcgcctggga atccccttcc
     3541 ggatgtcgca gccattcagg aggcaacaac gctcccaatg caatcacctt tttggtacgc
     3601 gacagttttt tgagcacttc gctcatttta ccctccagat gcaaaccgtt gtaaactact
     3661 atgtcagcct cggaaagcag tcctatatca ccctgagtag gcttgtacaa gtgcgggtcc
     3721 acgcccggcc ccatcagagc agtcacctgt gcggcatccc ccgctatgtg cgtcagcgcg
     3781 tcgccgatca tgccggtggt acacacaatg ttcagttttt ctgccccttg ctgctcgtcc
     3841 gtcgagcaag cggttgatac agcagccaag aagatgggaa tggccacgag tagttggatg
     3901 tcccgtaaaa atgatggaaa agacaagcgc attttgttcg ttattttcaa caggaacatc
     3961 gagattaggt ttttggttta aaaaaaatta gattagacta actttttatt caatcaatta
     4021 tttgcttacc tttggggcat tattacttaa ctacctctca ttgttatgaa aaaaaccttc
     4081 acactctttg tattcatgtt gctgttctct ttcacgggct ggtcgcaagc ctatatggat
     4141 gaaattgcca aaaaatgctg cgattgtgcc gcaaaggtca atcctgattt aaaagaaaat
     4201 gaattcgtta tggcaatcgg cgtctgtatg attgaagttg ccatgcctta cagaaaaaag
     4261 ttgaaaaaag atttcggagt ggacatgact cgtattgacc tggaaggaga aaaattgggg
     4321 gcagtcattg gcgctcacat ggttgctttt tgtccggatt tgcttatgag gctggccggt
     4381 gaagtgaatg acgaaaatgc cgtagaagaa caatcggccg tcctatctgc aaagggcatc
     4441 gtcaccagag tagagaaaga ttttcttgtt gtcttccaca ttaaagacga aacaggcaaa
     4501 accatcaagt gttattggat ggctgaaata gattccgacc tcgatctcgt tatggaatat
     4561 gagtctctgt cgggaaaaaa agtagaagtc cattatgtgg aagaaaaact cttcgatcct
     4621 cgtatatcag agtaccgccc gcattttgtt cttaccggct tgtatcgggc tgattgaaaa
     4681 gtgttagctt tgcccccgtt tcgaaaaacc tgcaaaacca ctcggtatga acctgcatga
     4741 atatcaaggc aaagaattat tgaaaaagta tggcgtagct gtacaggaag gcatcttagc
     4801 caacaacgta gaagaagcag tcgctgccta tggccagatc aaagcccgca ccggctccga
     4861 tttcgcattg gtgaaagccc aaatccacgc cggcgggcgc ggtaaaggcg gcggcatcaa
     4921 attggtgaaa aacttggacg acctcaaaga caaggccggc cagatcatcg gcatgatgct
     4981 caaaacgcct caaactcccg gcggcatgga cggcgccggc aaattggtac gcaaagtact
     5041 tattgccgag gatgcctacg cgcccgactt cgacgcctgc aaggaatact acgtctccat
     5101 ccttatggac cgcgaacgca aatgcaacgt catcgtctat tctaccgaag gtggtatgga
     5161 cattgaggct gtagccgagc atacgccgca cctcgttcac aaagagtaca tccatcccac
     5221 actgggcctc caggcttttc aggcgcgcaa aatcgccttc aacctcggcc tcagcggcaa
     5281 ggctttcaaa gaaatgacgg ccttcattca ctcgctttac gcagctttcc tcggcgccga
     5341 tgcatctttg tttgaaatca atccctgcct caaaacaggc gatgaccgca tcatcgcagt
     5401 ggactgcaaa gtgaccctcg acgacaacgc cctctaccgc cattccgatc tcgccgatca
     5461 acgcgacacc gacgaagaag accccaccga agtggaagcc aaaagctacg gtctcaattt
     5521 tgtcaatctc gacggcaatg tgggctgcat ggtcaacggc gcaggtctcg ctatggccac
     5581 catggacatc atcaagctct ccggcggcga cccggccaac ttcctcgacg tgggcggcac
     5641 tgccgatgcc aaacgagtgg aaaatgcttt ccgcatcatc ctccgtgacg agcgcgtcaa
     5701 ggccatcctc atcaatatat tcggcggcat cgtgcgttgc gaccgcgtag ctcagggcgt
     5761 agtggatgct tatcgcaaca tcggcgacat taaagtgccc atcatcgtgc gcctgcaagg
     5821 caccaatgcc gaccttgcca agcaaatcct cgacgagagc ggcctggcag ttcgttccgc
     5881 cattcaactt caggaagccg ccgatctggt gaannnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag gttgagtaaa cctgcctaaa
     6001 tgccaaccca aacacaaacc atgaccaacc aatccgtttc gcttcgcgct gccgacaaca
     6061 tccgcatcct cgctgccgct atggtggaaa aggccaagtc cggccacccc ggcgggccta
     6121 tgggcggcgc cgactttgtt catgtcctct attctgaatt tctgaattac gatccttccg
     6181 atatgagctg gccgctccgg gatcgcttct ttttggatgc agggcacatg tcggctatgt
     6241 tgtatgcaca gcaaaccctg ctcggaaatt acagcatgga cgatatccgc aatttccgtc
     6301 agtggggcag tcctacgccc ggccaccccg aacttgacgt agcccgcggc atcgaaaata
     6361 cttccggacc attgggattg ggccatgctt ttggtatcgg cgccgcgctg gccgagcgtt
     6421 ttctggcggc tcgtttcgga gaggaagttg cgcacaagac gtatatatat atatccgacg
     6481 gcggcgtgca ggaggagatt tcccaatcgg caggtcgcat tgccggcttc ctcggactgg
     6541 gcaacatcat catgttttac gatgccaacg acatacagct ctccactccg gtagaggccg
     6601 ttaccggcga agataccgcc atgaagtatc aggcttgggg ctggcatgta ctcaccattg
     6661 acggcaacga tcacgaccaa atccgccagg cacttcaggc aggcatcgct gaaacggcgc
     6721 gccctaccct catcatagga aaaaccctga tgggcaaagg cacccgcaca gccgacggcg
     6781 ctctgtttga aggcgaagta gaactgcacg gccaaccgct gagcggcgcc ggtgcttctt
     6841 ttgaaaaaac catcgccgga ctgggcggtc atccagacca acccttcgaa attttttccg
     6901 atgtagccga gtaccatcaa acggtattgg ataataaaaa gaaagcagcc aagaagcgaa
     6961 agtctgcttt caaaacctgg gctaagtcca atccggagca ggctgccaaa ttcaacttct
     7021 tcctctccgg gcagcttccc gacatcggct ggagcaacat ttcccttaaa cccaacgacg
     7081 ccacccgcaa tgcttccggc gcggtattgg ctcgcctggc cgaaagggta gaaaatatga
     7141 ttgtcgcttc tgcggatttg gccaacagcg acaaaacaga aaactttctt aaaaaaacaa
     7201 aggcttttgc acccggcgac ttctctggag cttttcttca ggccggcgtt tccgagttga
     7261 ctatggccgc cctttctatc ggcatgtctt tgcatggcgg cgtcatccct gcctgcgcta
     7321 ccttcttcgc gttttccgac tacatgaaac cggcgatccg ggtggcagcg ctcatggagc
     7381 aacctattat atttatatgg acgcatgatg ctttcagagt aggggaggac ggccccacgc
     7441 accagcccgt agaacaggag gcccagattc gcctcatgga gcaactgaaa aatcactccg
     7501 gacgcaacag tttcctcgcc ctccggcccg ccgacggcgc agaaacactt gaatcctgga
     7561 agctggcatt ggaaaatcgc cacggccctt ccggcctgat tctttcccgt cagggcattg
     7621 ccgacttgcc ggctgagact acggtaaaag cggcaggcac agccaagggc gcctacatcg
     7681 ttgaagactg cgagggcaag cccgacgtca ttctacttgc caatggttcg gaagtagcga
     7741 ctttaatagc aggcgccgcc cgtttgcgca acgagaaaaa tctcaaagtt cgcattgttt
     7801 ccgttccgtc tgaaggcttg ttcagaagcc agacgctcaa gtatcagcgc agcgtattgc
     7861 ccggcaatgt gcccgtgttc ggtcttaccg ccggcctacc cgtgactttg caagggctgg
     7921 caggcagcag gggccgcgta tttggcctga gccatttcgg ctactccgca ccgtttaaag
     7981 tattggatga aaaattcggc ttcaccggtg acaatgtctt cgatcaggtg ctccggttgc
     8041 tgaagtaagg tactgtctgt ggaggcagat ttatgattcg ataatccgaa ttctgcattg
     8101 tcccaccgcc taccgcccac cgcccactgc ctactgccta ccgcctactg ttttattctt
     8161 cccaaaaacc acaccacaat gcgcaagttc ctcattgcgt ccgattcctt taaggatgcc
     8221 ctgcctgccg cggaggtctg ccgggccatt gcacggggta ttcgccgagc tgttcctgat
     8281 acggatatta ttgaattgcc catgggtgac ggcggggaag gaaccgcccg cattattacg
     8341 cagcatgtag gcgggcaaat gttggatgta gaagccaaag accccttgtt cagaacaata
     8401 catacacagt atggcatttc atctgatgga aacactgcct atatagatat ggcggcagct
     8461 tcgggcttgc aactgctgag acctgaagag cgaaatccgc tttacactac ttcttacgga
     8521 accggagaaa tcattttaga tgcggtaaaa cggggcgccc gccatatagt gctctgcatc
     8581 ggcgggagtg cgacgcatga ctgtggcgcc ggaatggcgg aagcattggg ttatactttt
     8641 ctggatgccg gaggacagcc ggtaagcccc acagggaaca acctcattaa aatagcatat
     8701 atagacgctg ccggactgaa agtcaacctc gactggcttc gggtggatgt gttgtgcgat
     8761 gttgtcaatc cattatatgg ccccaacggc gctgcgcata tcttcgcccg ccagaaaggc
     8821 gccgatgata aagcggtgga attgttggag gaaggaacga agcatttttc cattctcctg
     8881 caagaacatt tggacaaaaa aacagcctcc atatccgggg ccggcgcagc aggaggcatg
     8941 ggggcaggag cttgggcttt tctgaacgcc catattatac ccggcactga atatgtttca
     9001 gaagtggcag gattgagaaa tgccatggca gaagccgata ttgtttttac cggagagggg
     9061 cgcttggatg aacaaaccat aaatggaaaa ctgatatccg gcattgttcg cgctgccgcc
     9121 gaatatcaga ttcctgtcat tgctttatgc ggtagcttgg gaatccaccc gggccaaatg
     9181 gaaagattgg gtattacggc agcttttgcc atcaacaacc gtccctgtac gctggccgaa
     9241 gcattagccg aaacagccgt caatctggaa cacctggcat tctctctggc atgtaccctg
     9301 gcggcaagtg aaagatacat tgccgtggca ggtatgggga gcggtaaaga attgaatcgc
     9361 cgggcagccg acatcctttc caagagagaa acggaggtgt tccaactgat agcgaaaggt
     9421 tttaccaacc cccaaattgc atccacgctg tttatcagta gccagaccgt tgcggtacac
     9481 cgaaaaaata taatgcgcaa attgggggtc aataaagtga ctgcactgct gaaaaaaggc
     9541 ttcgattcgg ggattttttt ctaaaaaagt gccggttaat gaaaaaatac cctgaacagg
     9601 gtataaaatc caatgccttt gatactatct ttgccccgtt tgaatttgtt cagcacctta
     9661 tctcattcat caaaagacat cttagtgagc tttctcgcgc aggcaacttc tannnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nctacttacc aaaaaaaaaa tcgctccttc tcttgcatta
     9781 ctggagcgaa ctaccctatc tttgccctcg caagcaaaat gcatcccatg aacataaatg
     9841 ttacgcctgc catctgtgca tcattatccc tttttacttg ggattcatac ccgtccttca
     9901 gcacacgtca cgctcatatt attttgatct gattcgatag cgttccattg gacgcttttc
     9961 gcatattgtt tagtctgcaa aaagttgcag gttcattttt tgaaagtctg ttttcttgtt
    10021 ctactgaaat aatacgacct acacacattt ttttaccgag tagcgcaaaa aacgcgttcg
    10081 cctacgacct ggctataacg catggcaact gcatactgcc aaccgcatac tgcaaactgc
    10141 ataccgcaca ctgcacaccg cacactgcct actccaccct catttggctt ttagcaactt
    10201 ttatatttcc taatctttaa caaattgtga tcttatggaa agaacgagtt ttacaacgaa
    10261 ccggtcttgg atcctgggca ttttgaccct ggtattggga ctggcgggat tccagagcgc
    10321 aaatgctcag tgtccgacgc ctacgggctt gacggcattg aacatcgggc agaattctgc
    10381 cacactgcgc tggacttcca acagcacgcc gaccgacaac tgctggacag taacggtggg
    10441 cggcatgggc gtggcaagct gcaacaacgc aggccaggcc acgattcaaa ccacagtgtg
    10501 ctacatcaac aacgtggtga gcttttctgc acctgtaacc ggcatgacgg ttgtgggtag
    10561 ccagatcaac gttacagttg gcggcttgca gcctggcacg gactatgagt actatgtagc
    10621 cgagacctgc gacggtatcg gtgcaccgct caacgtgtct gcatgcgccg gtcctgctgc
    10681 attcatcact ttggatgcac agtacacggt aacggctacg acggttcgtc cgagctgtcc
    10741 gttcatcagc ccgggctatg tagccaacgg ctcgtttacg gtaacggtga ccgacggcac
    10801 gacctgcgcc ggcacctaca cggtgaacgc tacgccggta gccggctccg gccctgccgg
    10861 ttctacgcct ccgagcacga cggtagtgac ctacatcggc ttcccgcagg gcgcattcct
    10921 gtttgccaat gcaggcgcgg gccagtacac agtaacggtt accgaaaccg gctcctgcaa
    10981 cccggctact gatccggtgg tgatcacggt gacagtaccg gacggtattg acaacacggc
    11041 tccggtttat tatgtaacgg acgtattggg caacattctg gccgacaacg acggcgctac
    11101 agcagccggc acgacccgca acttcggcac ggtagcagta ccggaaggcg aatgcggccg
    11161 tcaggatgag tactatgtgt atggtttcga caactgcgac ggtttcatca cggcgacgaa
    11221 cgcagtaagc gcatcagcag tgacagtacc gggcacgatc cagcctggca cgcaggtaag
    11281 cacgacgccc gacggcttcg gctttacgct ggtggacgta cactggagca caggtacgag
    11341 cacggtgacg attctgggcc gcgacgcagc aggcaacatt gccaacggcg cagcaggtct
    11401 tcagttgatc atgaacgtac cggacaacac ggatccggtg gtgagcattt tgggcaacag
    11461 ccagttcacg attccggtat gcgccacgag cgtaacaggc attttgacgt tccaggtgga
    11521 cgacctgtgc gatcagaaca acgtaaactt tgccaacctg attgttaact ttggcggcgc
    11581 tacgggcgtg ctgaacttca caggcaacaa ctaccgcgaa tacttggtaa cgttcccggc
    11641 agcaggcaat tatttggttt ctgcttctta cacggatgcc aacggcaacc aaggcttcat
    11701 tgatcaggtg attcaggtac agttggcagc agccaaccag ccggcaacga tccaagccaa
    11761 cgctgagacg gtaacgttgg catcttgcca gacttctgcc cagattgtat atagcttcac
    11821 gatctctgac gactgcgaac cgatcaacat cgccaacgta cagttcaacg gcggcggctc
    11881 cggccttccg aacctgaatg gcgctggttt cttctttact gatccggtag gcccgaacac
    11941 agtttacttt gaagtacagg gcaacgtagg cccggcgggt tcttatttcc cgctgatcac
    12001 ctacaacggc gtaactgcca acccgacgat cacggtgatt cagaacgcca accaaccggc
    12061 agacatcgta ttgccggcga tcaacgtaac gatcccgcag tgcgcttctt cagtggatgt
    12121 ggtcattccg atcacgatca cggacgattg cgacaatccg atcgtaccgg ctcgcgccag
    12181 cttcactttg ggtggtgtgg ccattactcc taacttcgtc aatgcagcag ccggctactt
    12241 cgagtttgct cgcacgctga cggcagccaa cagcggccag ttgctggcag ctacctacac
    12301 cgatgcacag ggtgctgttc gcacggtgga cgcactgatc acggtgaatg cacagcccga
    12361 cacctgggcg ccgatcatcg tgtatcctgc acaggacatc atcgtgaacc ttgattgttg
    12421 cggtcccagc acagcgatcg tcaattttga agctacggct acggacaact gcggcattgc
    12481 tgccttcact gccagctttg ctccgggcgt t
//
LOCUS       DAORLF010000080        12511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2684_length_12511_cov_1.987315, whole genome shotgun sequence.
ACCESSION   DAORLF010000080 DAORLF010000000
VERSION     DAORLF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_12511_cov_1.987315"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>362)
                     /locus_tag="PK198_08585"
     CDS             complement(<1..>362)
                     /locus_tag="PK198_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRF38832.1"
                     /translation="DSGHLSIIVFSMLIGGMVALVSRNGGMAGIVQSLSRLARSPRSA
                     QFTTWLLGVAIFFDDYANTLIVGNTMRPVTDKFRISREKLSYLVDSTAAPVAAVAFIT
                     TWIGAQLGYIDSGIANIE"
     assembly_gap    363..380
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..918)
                     /locus_tag="PK198_08590"
     CDS             complement(<381..918)
                     /locus_tag="PK198_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38833.1"
                     /translation="MKNRLILLIGGLFLSVVNLIAQSPDMFTNGIELKSYRLELNQGK
                     AVFTSPDSHGMAEIEINGEMQAVEFTQGIAPLPVSVDAKGTLLLLRSPQGKLSLHHVA
                     QLKNGNHRLRHIPLWLSVLPPLVAILLALIFREVISSLFAGVWTGAFIAGGMRIESPY
                     YFLMSFAEVVQKYVVAALN"
     gene            1166..3475
                     /locus_tag="PK198_08595"
     CDS             1166..3475
                     /locus_tag="PK198_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38834.1"
                     /translation="MKKVVLLFALAMTATVAMFAQTVLWSEPFNGGSNGWTVRTVQCG
                     NITGAHVGRWTLTSATVNGTAVSNLQAEFQVNTIEDYSVRFTDGTNRGEMQARYTLAG
                     NVLTSNLNSITIPLNSTIFNYNATNRQTLYFSNMSIAQDQYTAWGSTLIGVGSPSFTV
                     NGSTLTIVNTAGTVQLVFSKAADCGAMWWWSPNGSVRSGALFNAATGEVVINSPTAAN
                     GAMVLNADYLTTRGLSANVPSGPPPYPQYTSELISPRIDLSAVTANVSLSFYQLVRFL
                     NPATSAPATTLRTSYAISTDDGATWGDAINANTDLAVNTAQQSQRLFPLPGVQGSANV
                     RIKFIWSSDFYYWAIDDIALLEQPRHDMRVNTNFFAITPNFATPASQVEPWRFLADIE
                     NVGASTSTGVNLNLAITNSTSMAVAYDGNVVYGDVAAGYVDENRIFPTATNPANFPTG
                     NYAGRYQVTADSTDAFPNDNIINWSFVVTDSLFTKEGTRTRGIAPAAEPSFTYGNIYY
                     VPNGAGLYARYLSFGVTNANELTGRSVTTLLYKWQNANDNTTAEPAEYGGAPIAFNSY
                     TFTGTEGTNFITIPFDLDGNAIPLEDGYHYIATVQYFTEDNQSLFLGGNGVLNYGAMV
                     FGTDSLNQNRYHTALVVGQNSSPSFSTLAFGYGICPQIRMSIGDNPDLSSSAFITSVN
                     DITKENLLEVFPNPASEVVYLKLLLEETQDVQISIFDVQGHLLNTRKFDNVSRETIEV
                     PVRDLPAGTYLVRLATPNGINVRQIVVGR"
     gene            complement(3602..>4390)
                     /locus_tag="PK198_08600"
     CDS             complement(3602..>4390)
                     /locus_tag="PK198_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF38835.1"
                     /translation="GERCDVATLEWSQQLLGKPVIDHWWQTESGWPMLANSVGLELLP
                     VKPGSVSKPVCGFDIQVLDATGRQMPAGEEGIVVVKLPLPPGNLPDLWNDTVRFKQSY
                     LDPYPGYFFSGDGGYIDADGYVYITGRIDDIINVAGHRLSTAAMEEVVAAHPAVAECA
                     VIGVHDNMKGEVPMGFVVLKAGINIGEEQLEKELVALVREKVGPVAFFKQAIVVKRLP
                     KTRSGKILRKIMRNMADGKEFTMPSTIEDASVLSELEDTMREHQ"
     assembly_gap    4391..4405
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4406..>4656)
                     /locus_tag="PK198_08605"
     CDS             complement(<4406..>4656)
                     /locus_tag="PK198_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF38836.1"
                     /translation="SDVGWVVGHSYIIYGPLLHGCTTILYEGKPVRTPDAGAFWRVIE
                     EYKVNVLFTAPTAIRAIKKEDPEGSLRHQYDLNSLRYLF"
     assembly_gap    4657..4798
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4799..5500)
                     /locus_tag="PK198_08610"
     CDS             complement(<4799..5500)
                     /locus_tag="PK198_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF38837.1"
                     /translation="MSYQSTFDRSIRQPEAFWEEQAKAVSWFRFPEKILFKDEKGLDR
                     WYGGGRLNTAWLCLDYHVEAGRGEQVAIHYDSPTTGVKRSITYRELLDEVSRFAGVLK
                     SFGVEKGDRVVIYMPMVPQTVVAMLACARLGAVHSVVFGGFAPRELAIRIDDAKPKLL
                     ITSGGGIEISKVIEYMPIVNLAWEEATHKPDACIVLQRDFVPVPLVPGRDHDWNELMA
                     KAEPAAHVEVEATDPL"
     gene            complement(5823..7721)
                     /gene="acs"
                     /locus_tag="PK198_08615"
     CDS             complement(5823..7721)
                     /gene="acs"
                     /locus_tag="PK198_08615"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRF38838.1"
                     /translation="MTKVIKSFDEYKEVYQRSVEDPEGFWAEQAETFTWQKKWDKVVE
                     WDFHNLDVKWFVNGKMNITENCLDRHLADRGDQTAILWEANDPKAPNKTYTYRQLYEA
                     VNRTANALKANGIQKGDRVCFYMPMVPELAIAVLACARIGAVHSVVFAGFSASALADR
                     IKDSECKMVICSDFNDRGAKHIPVKAVVDEAIALGCDSVKTVLVHQSTGDPLAFNSAI
                     DKWWHEEVDHQSAECPAEQMDAEDMLFILYTSGSTGKPKGVVHTCGGYMVYTAYSFKN
                     VFQYDDGDIYWCTADIGWITGHSYIVYGPLLAGATTVMFEGVPTYPDAARFWQTIEKL
                     KVTHFYTAPTSIRALMAMGDQYNEGHDFSSLKVLGSVGEPINEEAWHWYQDKIGGGKL
                     PIADTWWQTETGGILITPLAGVTPTIPSYATFPMPGVQPCLVDTNGVELEGDNVEGML
                     CIKYPWPSIIRTTYGDHERCKQTYFSTFPGKYFTGDGARRDHDGMYRIIGRVDDVINV
                     SGHRIGTAEVENAINQHPQVVESAVVGYPHDIKGQGIYAYVIATGVISDEQQFIKEVL
                     AVVTKVIGPIVKPDKIQVVTGLPKTRSGKIMRRILRKVAAGETDSLGDTSTLLNPEIV
                     EEIKTGAK"
     gene            complement(7795..8178)
                     /locus_tag="PK198_08620"
     CDS             complement(7795..8178)
                     /locus_tag="PK198_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF38839.1"
                     /translation="MTDKNTVKILLVDDEPGILAALEFLVKNEGYQVFTATNGEQALA
                     LAQELVPNLIVLDVMMPGLDGFQTALRIRAIPALEDCRIIFLTAKGTMQDRRQGYDSG
                     AEIYLSKPFDNDQLIDAINELIEYG"
     assembly_gap    8352..8521
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8522..>8738
                     /locus_tag="PK198_08625"
     CDS             <8522..>8738
                     /locus_tag="PK198_08625"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRF38840.1"
                     /translation="GTLVSALARNASTEQGAAALSNALDRDHDGSILSNLAGLLGGQA
                     PAQDNRALNGAGILNHILGDRQSGAVDM"
     assembly_gap    8739..8919
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9109..9267)
                     /locus_tag="PK198_08630"
     CDS             complement(9109..9267)
                     /locus_tag="PK198_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38841.1"
                     /translation="MTSESSSSTMMDWVIFLVSLVIMILMLMFMNEWFWVALPFVLTY
                     LARALKVI"
     gene            9334..10101
                     /locus_tag="PK198_08635"
     CDS             9334..10101
                     /locus_tag="PK198_08635"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRF38842.1"
                     /translation="MSIFDKFQLAGKTAVITGASKGIGEGIARALGQAGAKVVVSSRK
                     QEAVEAIAAELRSEGIDALAVAANTGSADDLQRLAEKSLAHFGGVDILVNNAATNPVF
                     GPVEQTDAAAFDKIMEVNVKGPFELSKLLLPSMKARGGGSIINISSIGGISPEPMLGI
                     YSVSKSALMMLTKVQAKEWGAYGIRANVICPGLIKTKFSQALWSNDAILNHVLGQLPL
                     ARVGSVEEISGLALFLASDAAAYCTGAVFTADGGHTI"
     gene            10283..>10421
                     /locus_tag="PK198_08640"
     CDS             10283..>10421
                     /locus_tag="PK198_08640"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRF38843.1"
                     /translation="MASDIEIARSIQLKDIGAIAAQLGIGEDQIERYGRYKAKLPLHL
                     ID"
     assembly_gap    10422..10431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10432..11959
                     /locus_tag="PK198_08645"
     CDS             <10432..11959
                     /locus_tag="PK198_08645"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRF38844.1"
                     /translation="KAAKNKLILVSAISPTPAGEGKTTVSIGLAQGLNRIGKRTTAVL
                     REPSLGPVFGIKGGATGGGYSQVLPMEDINLHFTGDFAAIEKAHNLLAALIDNNLQNK
                     KNNLGIDPRTVAWKRVMDMNDRALRKLVIGLGGTTGGVPRETGFDITAASEIMAILCL
                     SDDLIDLKRRFGNIFVGFTYDKQPVFARDLHAPGAMAALMKDAIKPNLVQTIEGQPAI
                     LHGGPFANIAQGTNSIIATRMGLSLSDYVVTEAGFGFDLGAEKFLDIKCRTAGLSPNA
                     IVIVATIRALKYHGGAGLKNLTEPNPDAVAKGLPNLEKHLENAKLFNVPCVVAINKFT
                     SDSAEEIQVIQERCAELGVPVNVADVWGQGGAGTTDLASTVAALADGNTTPFTPLYDW
                     EWSVEEKIRTIATKYYGAGSVEFQAKAKTDLKRISTLGMDKLPVCMAKTQKSLSDNPD
                     LIGRPQGFTLTVREIELAAGAGFLVPITGEMMRMPGLPEVPAANGIDIDDEGNISGLF
                     "
     gene            12206..>12511
                     /locus_tag="PK198_08650"
     CDS             12206..>12511
                     /locus_tag="PK198_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRF38845.1"
                     /translation="MRIFFALMLTAALWACNSAEKTPAENTAPVVQDTSATVETPPAE
                     VQDTTPPAPYENIEWKLYTLAGQRPFKDAQMTLKMEKGKMTGSGGCNQYSGAYTVSEG
                     "
BASE COUNT         2777 a         3298 c         3137 g         2763 t
ORIGIN      
        1 ctcaatgttg gcaatgccgc tgtctatgta gcccaattgc gcgccgatcc aggtggtaat
       61 aaaggcaacg gcggcaaccg gcgcagcggt gctgtccacc aagtaggata gtttttctct
      121 ggatattcgg aatttatcgg tcaccgggcg catggtgttg cccacaatga gcgtgttggc
      181 ataatcgtca aaaaaaatag ccacgcccag cagccaggta gtaaactgcg cgctgcgcgg
      241 cgagcgggcc aaacgactca gcgactgcac gatacccgcc atgccgccat tgcgggatac
      301 caaggccacc atacccccga tgagcatgga aaaaacgatg atagacaaat gcccgctgtc
      361 gtnnnnnnnn nnnnnnnnnn cgtttaatgc agcaacaacg tacttctgca ccacttctgc
      421 aaagctcatc aggaaataat acggcgactc gatgcgcata ccgcctgcta tgaaagctcc
      481 ggtccacacg cctgcaaaca aagaggagat cacctcccga aaaataagcg ccagcaaaat
      541 agctaccaag ggaggcaata ccgatagcca aagtgggata tgacgcagcc ggtggttccc
      601 gtttttaagc tgcgcaacgt ggtgcaggct cagtttaccc tgcgggctgc gcaacagcaa
      661 gagtgttcct tttgcgtcca cggaaaccgg caacggcgct ataccctgtg tgaactctac
      721 tgcctgcatc tcgccattga tctcgatttc agccatgccg tgcgagtcgg ggctggtaaa
      781 gacagctttt ccttggttca gttccaaccg gtagcttttt agctcaatgc cattggtgaa
      841 catgtcaggc gattgagcaa tcaggttgac gacagacaaa aagaggccgc cgatgagcaa
      901 aataagtcgg tttttcatgt tttgaagtgt tccgtgaagg ttggtgttgc cgcagcaggc
      961 ccaagaaatg aagatttttc caaacaggca ataatgaaat ttatttttta tcgccggctt
     1021 gatgaatccg ggccgagtac atggaaatca acagatatgc agtgcccctg acataaagcg
     1081 tagaattttt attcaaacaa tcgccaaaga cttaagcctt tctttgtttc tttgcgacgc
     1141 taacaatcaa aaaccttcat agcctatgaa aaaagtagta ctcttgtttg ccctcgccat
     1201 gacggcaaca gttgccatgt tcgcacaaac cgttttgtgg tcggaacctt tcaacggcgg
     1261 ctccaatggc tggaccgtgc gcaccgttca atgcggcaac atcaccggcg ctcatgtggg
     1321 ccgctggaca ctcacttctg ccaccgtcaa tggaacggca gtatctaacc tgcaagcgga
     1381 atttcaggtc aataccattg aggattactc tgttcgtttc actgacggaa ccaaccgcgg
     1441 cgaaatgcag gctcgttaca cgctggccgg taacgtgctg acttccaacc tcaacagcat
     1501 taccattccg ctgaacagta ccattttcaa ctacaatgcc accaaccgcc aaacgctcta
     1561 tttcagcaac atgagcatag cgcaagatca gtacacggcc tggggttcta cgctcatcgg
     1621 cgtgggttcg ccttccttta cggtgaacgg cagtacgctg accatagtca atactgccgg
     1681 cacggtgcaa ttggtgttca gcaaggctgc cgactgcggc gctatgtggt ggtggtcgcc
     1741 caacggaagt gtgcgctccg gcgccctgtt caatgccgct acaggagaag tagttatcaa
     1801 ctcgcctacc gctgccaacg gcgctatggt gctcaatgcc gactacctca ccactcgcgg
     1861 cctcagcgcc aatgtgcctt ccggtccgcc gccataccca cagtacacca gcgaacttat
     1921 ttcgccgaga attgacctct ctgcggttac ggccaacgtt tcgctctctt tctatcagtt
     1981 ggtgcgcttc ctcaacccgg ctacctccgc acctgccacc acattgcgca cttcttacgc
     2041 catcagcaca gacgacggcg ctacttgggg cgatgcgatc aacgccaata ctgacttggc
     2101 agtcaatacg gcgcagcagt ctcagcgttt gttcccgctg cccggcgtgc aaggctcggc
     2161 caatgtgcgc atcaagttca tctggtcttc tgatttttac tactgggcta ttgacgacat
     2221 tgccctgctg gagcagcctc gccacgacat gcgtgtgaat accaacttct tcgccattac
     2281 gcccaacttt gccactccgg caagtcaggt agaaccctgg cgcttcttgg cagatattga
     2341 aaatgtaggc gcttcaactt ccacaggcgt caacctcaat ctcgccatca ccaactccac
     2401 ttctatggcc gtagcttacg acggcaatgt ggtatatggc gatgtggctg ccgggtatgt
     2461 ggatgaaaac cgcatcttcc ctacggctac taatcctgcc aacttcccca caggaaacta
     2521 cgcaggccgc tatcaggtca cggcagattc caccgatgcc ttcccgaatg ataatataat
     2581 caactggagc ttcgtggtga cggattctct ctttactaaa gaaggtaccc gtacgcgcgg
     2641 cattgcaccg gcagccgaac cgagtttcac ttacggcaac atttattatg ttcccaacgg
     2701 tgcaggttta tacgcccgct acctgtcgtt cggcgtcacc aatgcaaacg aactcaccgg
     2761 tcgctctgtg accaccttgc tctacaaatg gcaaaatgcc aacgacaata ccactgccga
     2821 acctgccgaa tatggcggcg cccccattgc gttcaactct tataccttta caggaacgga
     2881 gggcaccaac ttcattacca tacccttcga ccttgacggc aacgccatcc ctctcgaaga
     2941 cggctaccac tatattgcaa cagtgcaata ctttacggaa gacaatcaat ctctgttcct
     3001 cggcggaaac ggggtactca actacggcgc aatggttttt ggaaccgact ctctgaacca
     3061 gaaccgctat cacacagcgc tggtggtggg gcagaattcc agcccctctt tcagcacctt
     3121 agctttcggc tatggcatct gcccgcaaat tcgcatgtct atcggcgaca acccagacct
     3181 cagcagctcc gctttcatta ccagtgttaa tgatataacc aaggaaaacc tgttggaggt
     3241 attccccaac ccggcttccg aagttgttta tctgaagctg ttgctggaag aaacgcaaga
     3301 cgtgcaaatc agcattttcg atgtgcaggg gcacttgctc aatacccgca aattcgacaa
     3361 cgtgagccgc gaaaccatcg aggtgcccgt acgcgatttg ccggcaggca cttatttagt
     3421 gcgcttagca acacctaatg gcatcaacgt tcgccaaatt gtggtaggac ggtaagatac
     3481 tactaagtgt tttagcttat aagcaaacga gttgcgggat acatgttctc gcaactcgtt
     3541 ttttttgtaa ggggcgacaa aaaaataaag tggtagcccg cctgttttag ttacagaaca
     3601 actattggtg ctcccgcatc gtatcctcca gttccgacaa caccgaggca tcctctatcg
     3661 tcgaaggcat ggtaaactcc ttgccgtcgg ccatgttgcg catgatcttg cgcaggattt
     3721 tgcccgatct cgttttcggt agtcgtttta ctacaatggc ctgtttgaaa aaagccaccg
     3781 gccctacctt ctcgcgtacc aaagccacca actctttttc caattgctcc tctccgatgt
     3841 tgatgcccgc tttgagcacc acaaacccca tcggtacttc gcccttcata ttgtcgtgta
     3901 cgccgatgac ggcacactcg gccaccgccg gatgcgcagc caccacttcc tccatcgccg
     3961 ccgtcgagag ccggtgcccg gccacgttga tgatgtcgtc tatgcggcca gtgatgtaca
     4021 catagccgtc ggcatctatg tagccgccgt cgccggagaa gaaatagccg ggatacggat
     4081 cgaggtaaga ttgtttaaat cggacggtat cgttccacaa atcgggcagg ttgccgggcg
     4141 gcagcggcag ttttaccacc acaatgccct cctcgccggc aggcatttgc cggccggtcg
     4201 cgtcaagcac ctgaatgtcg aagccgcaca cgggtttgct caccgagccg ggcttcacag
     4261 gcagcaattc cagccccacc gagttggcca gcataggcca tcccgattcg gtttgccacc
     4321 agtggtcgat caccggtttg cccaatagtt gctgcgacca ctccaaggtg gctacatcgc
     4381 agcgctcgcc nnnnnnnnnn nnnnnaaaaa cagatagcgc aggctgttca agtcgtattg
     4441 atgccgcaga ctgccctccg ggtcttcttt tttaatggcc cgaatggccg tcggcgccgt
     4501 gaaaagtacg ttcactttgt actcctcaat aacgcgccaa aacgcgccgg catcgggtgt
     4561 ccgcacaggt ttgccctcat ataaaatggt ggtacagcca tgcagcagcg gcccgtaaat
     4621 gatgtacgag tggcccacca cccaacctac gtcggannnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     4801 gcggatcggt agcttccacc tctacatgag cagccggttc ggccttggcc atcagttcat
     4861 tccagtcatg gtcgcggccg ggcaccagag gcacgggtac aaaatcgcgt tgcaacacaa
     4921 tgcaggcatc gggtttgtgc gtggcttcct cccaagccag gttcacaata ggcatgtact
     4981 caatcacctt gctgatctca ataccgccgc ccgatgtgat gagcagtttc ggcttggcat
     5041 cgtctatgcg aatggccagc tcgcgcggcg caaaaccgcc gaacaccacc gaatgtacgg
     5101 cccccaaccg ggcgcaggcc agcatggcca ccaccgtttg cggcaccatc ggcatataaa
     5161 tcaccacgcg gtcgcctttt tctacgccga aacttttcag taccccggca aagcggctca
     5221 cctcgtccaa cagttcgcgg taggtgatgc tgcgttttac cccggtggta ggcgagtcgt
     5281 aatggatggc cacttgctcg ccccggccgg cttcaacatg gtagtccaaa cacagccagg
     5341 ccgtattcag ccgcccgccg ccataccagc ggtctagccc tttttcgtct ttaaaaagaa
     5401 ttttttcggg aaaccgaaac caggaaacgg ctttggcttg ctcttcccaa aaagcctccg
     5461 gctgccggat gcttcggtca aatgtggatt ggtaggacat gggttgtttg tttacagtat
     5521 ttttatggca gccgcccgct ctaccgcctc cccgaccctc caaaggaggg agaaagcagg
     5581 ctgcgggcgc cgggtgttcc gctcttatgc ggccactatg ctctatcccg ccggccgcat
     5641 atccgcttca ccccctgctg cggccaaacg cgatgggcca gcaagtcagg aggcaacccg
     5701 aagttgccgc ctgacctgct cccgcttgca caccctactt cgcgccggtt ttgatctgtc
     5761 ttcgccggcg cgaggttcta cctcgtgccg actatcctgc gggttctacc cgcttacaaa
     5821 ctctacttcg cgccggtttt gatctcttct acgatctccg gattgagcag cgtagaggta
     5881 tcgcccagac tgtcggtttc gccggcggcc actttgcgca aaatgcggcg catgatctta
     5941 ccggagcgcg ttttgggcaa accggtcact acttgtatct tgtcgggctt gacaatcggg
     6001 ccgatcacct tggtcactac cgccagcacc tccttaatga attgttgctc atcggaaatc
     6061 actccggtgg cgatgacata ggcataaatg ccctgccctt tgatgtcgtg cggatagccc
     6121 accactgccg attccaccac ctgtgggtgc tgattgatgg cgttttctac ctccgccgtg
     6181 ccgatgcggt gtcccgatac gttgatcacg tcatccaccc ggccaatgat gcggtacata
     6241 ccgtcatggt cgcggcgggc gccgtcgccg gtgaaatatt tgcccgggaa ggtgctgaaa
     6301 tacgtttgct tgcagcgctc gtgatcgcca taagtggtgc ggatgatcga aggccaggga
     6361 tatttgatgc aaagcatacc ctccacatta tcgccctcca attctacgcc attggtatcc
     6421 accagacaag gctgtacgcc cggcatcggg aaagtggcat aagaaggaat ggtgggcgtc
     6481 acacctgcca gcggcgtgat gaggatgccg ccggtttcgg tttgccacca ggtgtcggcg
     6541 atgggcagtt tgcctccgcc gattttgtcc tgataccagt gccaggcctc ttcgttgatc
     6601 ggctctccta cgctgcccaa tactttcagg ctgctgaaat cgtgcccttc gttgtattga
     6661 tcgcccatcg ccatgagtgc gcgtatggac gtaggcgccg tgtagaagtg tgtaactttc
     6721 agtttttcga tggtttgcca gaacctcgct gcatccgggt aagtcggcac accttcaaac
     6781 attactgtgg tggcgccggc cagcagcggg ccataaacga tgtaagagtg gccggtgatc
     6841 cagccaatgt cggccgtaca ccagtatatg tcaccgtcgt cgtattggaa tacattcttg
     6901 aaactgtaag cggtataaac catgtatccg ccgcaggtgt gtaccacgcc tttgggtttg
     6961 cccgtcgagc cggaagtata gaggatgaag agcatgtctt ctgcatccat ttgttcggcg
     7021 gggcattcgg cgctctgatg gtccacttct tcgtgccacc atttgtcaat ggcagagttg
     7081 aaagcgagcg ggtcgccggt agattggtga accaaaacgg tttttacgct gtcgcagccc
     7141 agagcgatgg cttcgtccac cacagctttt acgggaatgt gcttggcgcc ccggtcgttg
     7201 aagtcggagc agatcaccat tttacactcg gagtccttga tgcggtcggc cagtgcggag
     7261 gctgagaagc ctgcaaatac cacggagtga acggcgccga tgcgggcgca ggccaatacg
     7321 gcaatggcca gttcgggcac cataggcata taaaaacaaa cgcggtctcc tttctgaatt
     7381 ccattggcct tcagtgcatt ggcagttcta tttactgctt cgtacaactg gcgataggta
     7441 taagttttgt tcggagcctt agggtcattg gcctcccaca gaatagctgt ctgatctccc
     7501 cggtcggcca agtggcggtc taagcagttt tcagtgatgt tcattttacc atttacaaac
     7561 catttgacat ccagattgtg gaaatcccat tccacaactt tatcccactt tttttgccag
     7621 gtaaatgttt ctgcctgttc tgcccagaag ccttcgggat cttctacgct gcgttggtac
     7681 acttctttgt actcgtcgaa agatttgatc acttttgtca ttgttggtca ggttttgatg
     7741 gagcacaaat atatatagat atttatattt gtgcatataa tttgcaaata ttttttatcc
     7801 gtattcaatc agttcattga tagcatctat gagctggtcg ttatcaaaag gtttggagag
     7861 ataaatttcc gcgccgctgt cgtatccttg ccgccggtct tgcatagtgc ctttggcagt
     7921 gagaaagatg atccggcagt cttccaaagc aggaatggcc cgtatacgca gtgcggtttg
     7981 gaagccgtct aagccaggca tcatcacatc taatacgatg agattgggca caagctcttg
     8041 tgcgagtgcc agcgcctgct ctccgttggt ggcggtaaag acctggtatc cttcattttt
     8101 taccagaaac tccagtgcgg caaggattcc cggttcatcg tccaccaaca ggatttttac
     8161 agtgtttttg tcggtcatgt ctggtattta ttggtcaatc gtttggtttg gagcggcaat
     8221 ataggcgata tgatttttga ttgcaaaaga cttttgtcaa tataaaaaat gataaacctc
     8281 atttgttatc tttgccatga aattgatacg aaattcattc gttcggcaag ccaaaaggaa
     8341 tgtgactttg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nggtactttg gtcagtgctt tggctcgcaa tgcttcgact gaacaaggag ccgctgcttt
     8581 gtcgaatgcc ttggacaggg accatgacgg cagcatactc agcaatctgg caggtttgct
     8641 cggcggtcaa gcgcctgctc aagacaaccg cgcactcaat ggcgccggca tcctcaacca
     8701 catcctcggc gaccgccaaa gtggcgccgt tgacatgann nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cggccatgag catggtttcc
     8941 cgactactcg accgcgatgg cgacggcagc gctatggacg acatcgcccg tatcggcatg
     9001 aatgtgctgg ggaatttgtt caaaaagtaa tcggagtaca aaacaacgat cataaaaaag
     9061 ccgggcaact gtacgtcgtc cggcttttgt tttttggcgt ccctttgctc agatcacttt
     9121 gagcgctctg gccaaatagg tgagcacaaa gggtagcgct acccagaacc attcgttcat
     9181 gaacatgagc atcaatatca taatgacaag agagactaag aagattaccc agtccatcat
     9241 ggtggaagaa gaagattcgg aagtcataac tcattttgtt ttggtacggc aaaagtaaaa
     9301 aatccttttc tttacggcgc aaaaaaaacg aacatgagca tttttgacaa atttcaactc
     9361 gcaggcaaaa ccgccgtcat caccggcgcc agcaaaggca tcggtgaagg cattgcccgc
     9421 gccctggggc aggcgggcgc aaaagtggtg gtgagcagtc gcaaacagga ggcagtagaa
     9481 gctattgccg ctgagttgcg ctcagaaggc attgatgctc tggctgttgc agccaataca
     9541 ggaagcgccg acgatctgca acgcctcgca gaaaagagct tggcgcattt cggcggcgtg
     9601 gacattttgg tcaataatgc agctaccaat cctgtttttg ggccggtgga acaaaccgat
     9661 gcggccgctt ttgacaaaat catggaagtg aatgtcaaag gcccatttga actgagtaaa
     9721 ctcttattgc cttctatgaa agcgcgcggc ggcggctcca tcatcaacat cagcagcatc
     9781 ggcggtattt cgcccgaacc catgctgggc atttacagcg tgagcaaatc tgctctgatg
     9841 atgctcacca aagtgcaggc taaggagtgg ggagcttacg gcatccgggc caacgtgatt
     9901 tgtccggggc taattaaaac caagtttagt caggcgcttt ggagcaatga tgccatcctg
     9961 aatcatgtgc tggggcaatt gccgctggca cgggtaggct cggtggagga aatatcgggg
    10021 ctggcgctgt ttctggcatc cgatgcggca gcgtattgta cgggtgcggt gtttacggca
    10081 gatggggggc atactattta aagtttgcgg tagacggtag gcggcgccat cgtggagcgt
    10141 tcttctccag cttagtgaag gggtgactgc cccactggaa cttgctgagt caccccttca
    10201 ctacccccga agcccggaac ctgaaacctt gggatcgaaa cacgaaacat ttttttgaac
    10261 caaataaaaa ccaaccaaga tcatggcttc agacatcgaa attgccagaa gtattcaact
    10321 caaagacatt ggcgcgatag ccgctcagct cggcatcggg gaagatcaga tagagcgcta
    10381 cgggcgctac aaagcgaaac tgccactgca tctcattgac cnnnnnnnnn ngaaagcagc
    10441 aaaaaacaag ctgatactcg tgtcggccat atcgcctacg ccggcgggcg agggcaaaac
    10501 caccgtttcg ataggcttgg cgcagggcct caaccgcatc gggaagcgca ctacggctgt
    10561 gttgcgggag ccttcgctgg ggccggtttt cggtatcaaa ggcggggcca ccggcggagg
    10621 atattcgcag gtgttgccga tggaagacat caacctgcac ttcaccggcg attttgcagc
    10681 catagaaaaa gcgcacaacc tgctggccgc cctgattgac aacaacctgc aaaacaaaaa
    10741 gaacaacctc ggcattgacc cccgcaccgt ggcctggaaa cgggtgatgg acatgaacga
    10801 ccgcgctttg cgcaaactcg tcatcgggtt gggcggcact acgggcggcg tgccccgcga
    10861 aacggggttc gacatcacgg cggcctctga aatcatggcg atactttgtc tctcagacga
    10921 cctgattgac ctcaaacgtc gcttcggaaa tatttttgta ggattcacct acgataagca
    10981 gcccgttttt gcccgcgacc tgcacgctcc cggcgccatg gctgcgctga tgaaggacgc
    11041 catcaagccc aacctggtgc aaaccattga agggcagccg gctattttac acggtggccc
    11101 gtttgccaat attgcgcaag gcaccaattc catcattgcc acccggatgg ggctgagtct
    11161 gtcggattac gttgtaacgg aagcgggctt cggtttcgat ctcggcgccg agaaattttt
    11221 ggatatcaag tgccgcacgg cggggctttc gcccaatgcc atcgtgatag tggccaccat
    11281 ccgggcattg aaatatcatg gcggcgcagg gctgaaaaac ttgaccgaac cgaatccgga
    11341 tgcggtagca aagggcttgc ccaacctcga aaagcatttg gaaaatgcca agcttttcaa
    11401 tgtgccctgc gtagtggcca tcaataagtt tacttccgac agcgccgaag agattcaagt
    11461 gattcaggag cgttgcgccg aattgggcgt gccggtgaac gtggccgatg tatggggtca
    11521 gggcggcgcg ggtactaccg atctggcctc gacggtggcg gcattggcag acggcaacac
    11581 cacgccgttt acaccattat atgattggga atggagcgtg gaggaaaaaa tacgcaccat
    11641 tgccactaaa tactatggcg ccggctcggt ggagtttcag gcgaaagcca aaaccgacct
    11701 caagcggatc agtacgctgg gcatggacaa gctgccggtg tgtatggcca aaacgcaaaa
    11761 gtcgctttcc gacaatcccg atctcatcgg tcgtccgcag ggtttcacgc tcaccgtgcg
    11821 cgaaatagag ctggccgccg gggcgggctt tttggtgccc atcaccggtg agatgatgcg
    11881 catgccggga ttgccggaag tgcctgctgc caacggtatt gatattgacg acgagggcaa
    11941 tatcagcggc ttgttctgag ggtgattgcc ttgcgcgcca gggaccgcag cggatacctg
    12001 cctgctgcac aaccccgttt ttttttgggg gggcagcagg caggtatgga gcgaagggcc
    12061 cgcccgccgg tttgtatgct tggatatggt ggagatgggg agaggcgggg gcgcccaaaa
    12121 aatcatatct caaaataggg gattcgttta cctttgcgcc ccgaaacccg gaccatcggg
    12181 aaggaatcaa aaaaacatga acacaatgag aatctttttt gctttgatgt tgacggcagc
    12241 cctgtgggct tgcaacagcg ctgaaaaaac gcctgcggaa aataccgctc ctgttgtgca
    12301 agataccagc gccacagtgg agacgccacc tgcggaggtt caagacacta cgccgccggc
    12361 gccttatgaa aacattgaat ggaagctgta cacactggcg ggccaaaggc cgttcaagga
    12421 tgcgcagatg accctgaaaa tggaaaaggg taagatgacc ggatcggggg gctgcaacca
    12481 gtacagcggc gcctatacgg tgagtgaagg g
//
LOCUS       DAORLF010000081        12500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2687_length_12500_cov_2.115950, whole genome shotgun sequence.
ACCESSION   DAORLF010000081 DAORLF010000000
VERSION     DAORLF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_12500_cov_2.115950"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>831)
                     /locus_tag="PK198_08655"
     CDS             complement(<1..>831)
                     /locus_tag="PK198_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF38846.1"
                     /translation="RFRYLLVDEFQDTNHLQYAIVRKLVNYDHSPRNVCVVGDDAQSI
                     YAFRGATIQNILDFEQDFKPFGIKVFKLEQNYRSTEHIVQSANEVIMHNSRQIQKKIW
                     SDKGQGQRVKIIKARTDTEEGHRVVDTIIEQKNRYHLSNNEIAVLYRTNAQSRIFEEY
                     LRRYNVPYRIFGGQSFYQRKEVKDLISYLRLAVNPGDEEALRRVINFPRRGIGETSVD
                     KISELAGQTNRRMWDCIPEVEMATRTRSAVADFLEHERRAVAREQDALDQFTPFKKGE
                     "
     assembly_gap    832..844
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<845..1489)
                     /locus_tag="PK198_08660"
     CDS             complement(<845..1489)
                     /locus_tag="PK198_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF38847.1"
                     /translation="MSHYLNELNEVQRNAVTNINGPVLVVAGPGSGKTRVLTYRIAHI
                     IEQGAAPWEVLALTFTNKAAREMKELIAGVVGPQASQVWAGTFHSIFARILRSEAEKI
                     GYPSDFTIYDTEDTKNVISAIVKEMNLDRETYNVNTLRSRISAAKSSLITPKLYQDNE
                     DLREEDRRANRPLTYSVYEKYTARCKRSGAMDFDDLLYRLYELVQRNPDNILEKY"
     gene            2052..3707
                     /locus_tag="PK198_08665"
     CDS             2052..3707
                     /locus_tag="PK198_08665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF38848.1"
                     /translation="MHKYFFSILLALTALTACAQDDFYKSYSFNRADTLRGMLRPERT
                     CYDVTFYDLNIRVDPKSQSISGYVDIAFRTVEDFSVLQIDLFENMKLSRIEFQGRELS
                     FRREHAAVFVTFPPQSKGGSGAFRVYYEGAPQVARNAPWDGGFVWNKDRLGNHWIGVA
                     CEGDGASLWWPNKDHLSDEPDSMSIRITVPNGLMAVANGNLRQTVRDGDQTCYEWFVS
                     YPINNYNVTLNIAKYAQFSDTYIAKDGEKLALDYYVLEYNLKKAKEHFKQVHSVLACY
                     EHFFDKYPYWRDGFALVETPYLGMEHQSAIAYGNKYMRGYLGGMIPAGMDWDYIIVHE
                     TGHEYFGNSISCNDLSEMWIHESFTTYMEALYVEYMYSYKEAVQYLLSQRRNIRNREP
                     ILGPAHVNWENWEGSDHYYKGAWILHTLRSTLRDDAKWFDLLKGFYQKHAHSNIHSEA
                     FFQYVNTATGRNYDAFFRQYLRYPGIPTFEYELEPKGNGLRIRYRWKADVSGFNMPVA
                     LGKKDAYEWLQPTAEWQTTTLKNITEDEFRVATELLLINEKRK"
     gene            3837..4385
                     /locus_tag="PK198_08670"
     CDS             3837..4385
                     /locus_tag="PK198_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38849.1"
                     /translation="MKHLLFISLFALSSMSLRAQSNDQPPFNFGLELDALPYLTGGYF
                     GAAWAGRQHWRLRVLTAGVNKPDWSTPDGFTNHRITAYATVADYFLKPDWTGWWIGGG
                     PVFWSSTIQTDAKLQTAQFKNLLLNGSIGYNIRLPHHFYLSPWAGLSIRVAGDTDVPV
                     DNTTFTLPLFNPEASLKLGWYF"
     gene            complement(4544..5824)
                     /gene="hisD"
                     /locus_tag="PK198_08675"
     CDS             complement(4544..5824)
                     /gene="hisD"
                     /locus_tag="PK198_08675"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRF38850.1"
                     /translation="MKITRFPQCSDWPALAARPAENAADLDELVRRVFDDIRTEGDAA
                     LRRYTAQFDRCELDTLLVGQEEVSGSAELVSPELREAIALAAGNIERFHAAQREPVQV
                     IETSPGVACRRESRPIERVGIYIPGGSAPLFSTVLMLGIPARLAGCKEVVLCSPPGQD
                     GRIHPAILYAASVVGVTSIVSVGGIQAIGAMALGTETVPRVDKIFGPGNQYVTAAKQF
                     AQRNGVAIDMPAGPSEVLVIADAGAPPAFVAADLLSQAEHGPDSQVVLLSDNEDLILA
                     VAEETARQLELLPRRDIARRALENSRAILLPSLRECMEFSNLYAPEHLILAVADAEAL
                     ASQVSNAGSVFLGYYSCESAGDYASGTNHTLPTNGYARNYSGVSLDSFVKKITFQKLS
                     AEGIRNIGPAIELMAEAEALTAHKNAVTVRLAAL"
     gene            complement(6054..6269)
                     /locus_tag="PK198_08680"
     CDS             complement(6054..6269)
                     /locus_tag="PK198_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38851.1"
                     /translation="MSAIHYVTDDAGSPVAVQVPIADWNLFQKEFDEMKRALEVLHGI
                     KNALEEVEQVKQGKRKLQTLSDFLHEC"
     gene            complement(6467..7063)
                     /gene="yihA"
                     /locus_tag="PK198_08685"
     CDS             complement(6467..7063)
                     /gene="yihA"
                     /locus_tag="PK198_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRF38852.1"
                     /translation="MTIHSAEYTGSYPSELTCPKDNRREFAFIGRSNVGKSSLINLIT
                     GRRDLALTSGKPGKTQTINFFLINKDWYLVDLPGYGYARTSQTNRRKWEIMIQGYLSR
                     RANLVCTFVLLDSNIPPQNIDIEFINWLGEKGLPFVIVYTKTDRLTDAQREQNITRLR
                     EALLEHWNVLPQEFVTSAAKHLGGGAILDFIESIPEEV"
     gene            complement(7244..>8552)
                     /gene="lnt"
                     /locus_tag="PK198_08690"
     CDS             complement(7244..>8552)
                     /gene="lnt"
                     /locus_tag="PK198_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRF38853.1"
                     /translation="TAFHHTQRRMPRIAGAAFMAYWMAFEYLHLNWEITWPWLTLGNA
                     FAQFPALVQWYEFTGVFGGGLWILVGNWLIFNAWKQRNDRAALRSSVVKTALWILVPT
                     GISLWQYFTYQEEGDKTVQVALVQPNYEPHYEKFDIPEDVQMQRFLELSASVTDSKTD
                     YLVWPETSFGYVETRSVESYPAVVQLRGFMEKHPQLKVIAGIDAYHIFLPGEPHSKAV
                     RSSNRSGGGTMYFEVLNAAAQFSNESEEVQLYRKSKLVPGPEILPYRRFFGVFEPVIK
                     QVQGTVEGVGTQKERMAMSSDKARLAPVICYESIFGEFFTGYIHKGAQAAVIMTNDGW
                     WDNSPGHRQHLWLGTLRAVETRRSIARAANTGISAFINQRGDILQATRYNEAIAIKGQ
                     VRLNDRVTFYVRWGDLIARIAVFTTLLLLLNTIAKGIQARVGR"
     assembly_gap    8553..8567
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8568..8938)
                     /locus_tag="PK198_08695"
     CDS             complement(<8568..8938)
                     /locus_tag="PK198_08695"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38854.1"
                     /translation="MLWGHRPLLLFIALWILAQALLCGRKNGAAGWRRAGLSSLSGLL
                     LGLGFPGIVPAPLLMFVGFVPLLLLESEISGSDAKGKGKQVLLHSYNAFLIWNILSTW
                     WVANTALAAGIVAMALNSLFMA"
     gene            complement(9028..10353)
                     /locus_tag="PK198_08700"
     CDS             complement(9028..10353)
                     /locus_tag="PK198_08700"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HRF38855.1"
                     /translation="MDPLKQLFHEKATAQRNAIRAMLKEHGELVVDQVTLGQIYGGAR
                     SIKMMVWETSDLDAEEGIKFRGYSIPELREILPKGQDKKEPRPEGLFWLMLTGEIPTE
                     EQVEWLTLQWTHRSNVPEHVFHTLDALPISTHPMTQFIIAVTAMQSESCFARRHEEVL
                     HKEEYWDATYEDVMNLIARLPRVAAYIYRRSFHGGDHIAPDISKDWGGNFAHMLGFDQ
                     PDFRSLMRLYLTIHADHEGGNASAHTCHLVGSTLSDAYLAFAGAMTSLAGPLHGLANQ
                     EVVGWIYKMLDALGTKTPTKDQIAAYVKATIDAGQVIPGYGHAVLRKPDPRFMAQKSF
                     AERYIKHDDMVQVVWDVFETVPPILESLGKVKNPWPNVDAHSGALLIHYGLTEFNFYT
                     VLFGVSRALGVLAQLAWSRALGFPLERPKSVTTQWAEKQVAKHLQANHA"
     gene            complement(10564..10986)
                     /locus_tag="PK198_08705"
     CDS             complement(10564..10986)
                     /locus_tag="PK198_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38856.1"
                     /translation="MKKFLIGMIAFLSVCSAFAQSAADQQSARKATEELTAVYSLSEA
                     QVQKMYVIQERRFRNLSEIESLKTTDESLYRQKLKSIRTLTESSIANMLTPEQKPILD
                     RERAERRRSEAARIEALKKSGASKDAIHEALLEMESDH"
     gene            11098..>11973
                     /locus_tag="PK198_08710"
     CDS             11098..>11973
                     /locus_tag="PK198_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38857.1"
                     /translation="MNILPKLLLTIVLLLSVAPALLAQKAAYRHYYRGAMLYAEGQFR
                     QAEGHFRQAAEIIPDNFYFTSAYALALSRSGQYESGRLLLQKAGGFIALKDPERVSKL
                     ASWHFFQGMSHLYGGRAGYAAAPIRKAIELMQAKPDGRQLSILYNALGYATLLHQGQG
                     SHVKAGIAPHFHVQRRSMERAAQYFEEALRHDATNAVALQNYRTLADTLGLPPGQFSA
                     QADSLNQRRLLDPIFSSMPANIDRTLQLGQFEEVLFLVDISGSMVMEKVICLNQDRFD
                     VMKEMALYLTDKMPET"
     assembly_gap    11974..12147
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12222..>12500
                     /locus_tag="PK198_08715"
     CDS             12222..>12500
                     /locus_tag="PK198_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38858.1"
                     /translation="MGDLDICEWAAGLAGKNITIHVLTFLESGLHNTGAFAEYTCLAE
                     NTGGKVVYIDNYRCTTQLFDFNLLETCNLAIPPLQRSQCWGVAVKDLWA"
BASE COUNT         2690 a         3443 c         3358 g         2807 t
ORIGIN      
        1 tctcaccctt tttgaagggc gtgaactggt ctagtgcatc ttgttcacgg gcaaccgccc
       61 gacgctcatg ctcaagaaaa tcggcgacgg cgctgcgggt tcgggtggcc atttccactt
      121 cggggatgca gtcccacatg cggcggttgg tttgtccggc caactcgctg attttgtcca
      181 cgctggtctc gccgatgccg cggcggggga aattgatgac gcggcggagg gcttcttcgt
      241 cgccgggatt gacggcgagg cggaggtagg agatgaggtc tttaacttcc ttgcgctggt
      301 agaacgactg cccgccgaag atgcggtagg gcacgttgta gcggcggagg tattcttcaa
      361 aaatgcgcga ctgtgcgttg gtgcggtaca gcacggcgat ctcgttgttg ctgaggtggt
      421 agcggttttt ttgctctatg atggtgtcca ccacgcgatg gccttcttcg gtgtcggtgc
      481 gcgctttgat gattttgacg cgctgtccct ggcctttgtc cgaccagatt tttttctgaa
      541 tctggcggct gttgtgcatg atgacctcgt tggccgattg cacgatgtgc tcggtggagc
      601 ggtagttttg ttcgagcttg aacaccttga taccgaaggg tttgaagtcc tgctcgaagt
      661 cgaggatgtt ctgaatggtg gcgccccgga aggcgtagat gctctgcgcg tcgtcgccta
      721 ccacgcatac attgcgggga ctgtggtcgt agttgacgag cttgcgcaca atggcgtact
      781 ggaggtggtt ggtgtcctga aactcatcta cgaggaggta ccggaagcgc tnnnnnnnnn
      841 nnnngtattt ttcgaggatg ttgtcgggat tgcgctggac gagttcgtag aggcggtaga
      901 gcaggtcgtc gaagtccatg gcgccggagc gtttgcagcg ggcggtgtat ttttcgtaaa
      961 ccgagtaggt gagcgggcgg ttggcgcggc ggtcttcctc gcggaggtct tcgttgtcct
     1021 gatagagttt gggagtaatg aggctgcttt tggccgccga aatgcgcgag cggagggtat
     1081 tgacattgta cgtctcgcgg tccaagttca tttctttgac gatggcgctg atgacgtttt
     1141 tggtatcctc tgtgtcgtag atggtgaaat cggaggggta gcctattttt tcggcttcgc
     1201 tgcgaaggat gcgggcaaag atggagtgaa aagtacccgc ccatacctgc gatgcctgcg
     1261 gccctaccac gccggcaatg agttctttca tttcgcgggc ggctttgttg gtaaaggtga
     1321 gggccagcac ttcccagggc gcggcgcctt gctcgatgat gtgggcgatg cggtaggtga
     1381 gcacgcgggt tttgcccgat ccggggccgg caacgaccag cacggggccg ttgatgttgg
     1441 tgacggcgtt gcgttgtact tcgttcagtt cgttcagata gtgactcatg tatggagttc
     1501 tttttggaat gtattggtaa tctgcttccc gcgccttgcg cggttcgcgg taatctactt
     1561 cttagtttat ctgcttcgca gtttatctac tgcgtagtgt atctactgcg tagtgtatct
     1621 gctgcgcagt ttatctgctg tgcggaaccc gaaacctgga accttgaacc acgacttcgt
     1681 cgtggcgagg cccggaacgc gaatttttaa cgcgaatctt ggcgaagggc gcgaatttcc
     1741 gcgaaccacg cctgtcgtcg tggcgcggtg cgagagggaa cacagcttgc agcttgaagc
     1801 cagtagccag cagccgttcc aacccgaaac ctcaaacccg aaatcagaaa tccgaaatcc
     1861 gaaatccgaa atcgggaatc cgaaatccga aatcaacccc ttcccaccag caacccgcgc
     1921 tttgcaactc gtagctcgaa gctcgcggct cgcagctttc ccaaagaggc aaaggtgcat
     1981 aaaaatcgcc acccccggca tacaaaaccg caagaaatgc gttacttggt gcgacaaaaa
     2041 aaatacacca aatgcacaag tatttctttt ccattttgtt agcccttacg gctctcaccg
     2101 cctgcgctca ggacgatttt tacaaatcct actccttcaa ccgggccgac accctgcgcg
     2161 gcatgctccg tcccgagcgc acctgctacg acgtcacttt ttatgacctc aacatccgcg
     2221 tggaccccaa atcccaatcc atctccggat atgtggacat cgccttccgc actgtcgagg
     2281 atttcagcgt gctgcaaata gacctctttg aaaacatgaa actctcgcgc atcgagtttc
     2341 aaggccgcga actcagcttc cgccgcgaac acgccgccgt ttttgtcacc ttcccgccac
     2401 aatccaaagg cggcagcggc gctttccgcg tctattacga gggcgccccg caagtagccc
     2461 gcaatgcccc ctgggacggc ggcttcgtgt ggaacaaaga ccgcctcggc aatcactgga
     2521 tcggcgtggc ctgcgagggc gacggcgcca gcctctggtg gcccaacaaa gaccacctca
     2581 gcgacgaacc cgacagcatg tccatccgca tcaccgtgcc caacggactc atggctgtgg
     2641 ccaacggcaa cctccgccaa accgtccgcg acggcgacca aacctgctac gagtggtttg
     2701 tttcctaccc catcaacaac tacaacgtca ccctcaacat cgccaaatac gctcagtttt
     2761 cagacaccta catcgcaaag gacggcgaaa aactcgccct cgattattat gtgctggagt
     2821 acaatctcaa aaaagccaaa gaacacttca aacaggtaca ctccgtgctg gcctgctacg
     2881 agcacttctt cgacaagtac ccctactggc gcgacggctt cgccctcgtc gaaacgcctt
     2941 acctcggcat ggagcaccaa agcgccatcg cctacggcaa caaatacatg cgcggctacc
     3001 tcggcggcat gatccccgcc ggcatggact gggactacat catcgtacac gaaaccggtc
     3061 acgagtactt cggcaacagc atcagttgca acgacctctc cgaaatgtgg atccacgaat
     3121 ccttcaccac ctacatggag gccctctatg tggagtacat gtacagctac aaagaggccg
     3181 ttcaatacct cctcagccag cgccgcaaca tccgcaaccg cgaacccatc ctcggtccgg
     3241 cgcatgtcaa ctgggaaaac tgggagggca gtgatcacta ctacaaaggc gcctggattc
     3301 tgcacaccct gcgcagcacc ctccgcgacg acgccaaatg gttcgatctg ctcaagggtt
     3361 tttaccaaaa acacgcgcac tccaacatcc actcggaggc gtttttccag tacgtcaata
     3421 ccgccaccgg ccgcaattac gacgccttct tccgccagta cctccgctat cccggcattc
     3481 ctacattcga gtacgaactc gaacccaagg gcaacggact gcgcatccga taccgctgga
     3541 aagccgatgt atcgggcttc aacatgcccg tagcgctggg caaaaaagac gcctacgaat
     3601 ggctccaacc caccgccgaa tggcaaacga ctaccctcaa aaacataacg gaagacgagt
     3661 ttcgcgtggc tacggagctg ttgctgatca acgaaaagcg caagtagcag cgactttagt
     3721 cgccgaaaac acgccaagcc aaagtacttg tttacagaca gctatacctt tcaatgctgc
     3781 cgtattttaa tacatttgcc ttgagatact ttccgatcag cctcaacctt caagtcatga
     3841 aacatcttct gttcatttcc cttttcgccc tgagcagcat gtccttgcgc gctcaatcca
     3901 acgaccagcc tcctttcaac tttggattgg aattagacgc cctgccctac ctcacaggag
     3961 gttatttcgg agcagcatgg gcagggcggc aacattggcg gctcagagta ctcaccgccg
     4021 gcgtcaacaa gcccgactgg tccacgcccg atggctttac caaccaccgc atcacggcct
     4081 acgcgacagt tgccgactat tttctgaagc ccgattggac cggctggtgg ataggcggcg
     4141 ggcctgtttt ctggagcagt acgattcaaa ccgatgccaa actacaaacg gctcaattca
     4201 aaaatctgct cctcaacggc agcataggct acaacattcg cttgccgcac cacttctacc
     4261 tctcgccctg ggccggcctc agcatccggg tggcaggaga cacagatgtg ccagtggaca
     4321 atactacttt tacgctgccg ctgttcaacc cggaggcatc gctcaaattg gggtggtatt
     4381 tttaatgcaa tatcttcaaa agtcattttg ggggcgtgcc cccgcaccac caccgcctgc
     4441 cctgcactcc aaaggaaccc gccggcaggc gacaagcagc ggggtcgggt gcctcagtcg
     4501 ctcctcctaa ggtcgtcact accttcggcc ccctcacgcg caactacagc gccgccaacc
     4561 tcaccgtcac cgcatttttg tgcgccgtca gtgcctccgc ctccgccatc agttctatgg
     4621 ccgggccaat attgcgaatg ccctcggcgc tgagtttctg aaaagtgatt tttttaacga
     4681 aactgtccaa tgatacgccg ctgtagttgc gcgcatagcc gttggtaggc aaggtgtggt
     4741 tggtgccgga agcataatcc ccggcgcttt cacaactgta atatcccaaa aacacagaac
     4801 cggcattgga tacctgcgag gccagagcct ccgcatcggc caccgccaga atgaggtgct
     4861 ccggggcgta taaattgctg aattccatgc actcgcgcaa agaaggcagc agtatggcgc
     4921 ggctgttttc gagcgcccgt cgggcgatgt cgcggcgagg cagcagttcc aattggcggg
     4981 ccgtctcttc cgccacagca agtatgaggt cttcgttgtc cgacaacaaa accacctggc
     5041 tgtccggccc atgctcggct tgcgaaagca ggtcggcagc cacgaaagca ggcggcgctc
     5101 cggcgtcggc tatcacgagc acctcgctgg ggccggcggg catgtctatg gctacgccgt
     5161 tgcgctgggc aaactgtttg gccgcagtaa cgtactgatt tccaggccca aaaattttgt
     5221 ccactctcgg cacggtctcc gtgccgaggg ccattgcccc gatggcctga atgccgccca
     5281 cgctgacaat cgaagtgacg cccaccaccg aggcagcata caaaatggcc gggtgaatgc
     5341 gcccgtcctg gcccggcggc gaacaaagca ccacctcttt gcagccggca aggcgcgccg
     5401 gaatgcccag catcagcacc gtggaaaaca agggcgccga gcctcccggt atgtaaatgc
     5461 ctacccgctc aatggggcgg ctctcgcggc ggcaggccac gccaggactg gtttctatta
     5521 cctgcacggg ttcgcgctgg gcagcatgga agcgctctat gtttccggcg gccaaggcga
     5581 tggcctcgcg cagttcgggc gatacaagct ctgcgctgcc cgatacttct tcctgcccca
     5641 ctaagagcgt atccaattca cagcggtcga actgagcggt gtagcggcgc aaagcggcat
     5701 cgccctctgt gcggatatca tcgaatacac ggcgaaccaa ttcgtcgagg tcggcggcat
     5761 tttcggcagg ccgggcggcc agagcgggcc agtcgctgca ctgcggaaag cgagtgattt
     5821 tcatggttga taggattgac gtggggggat aacaaaaaag gagggttttt gatgcgggaa
     5881 gaacccgcaa aaagtttcat atctcacggg gatgaaaaac aattggaagc ctgtcaatca
     5941 tgcaattatg agtcttagga ttcgtcattg tcctcggttt gttcgggtat ctgcacagct
     6001 acattttgct cttgatgata actgtcaatg agatgagcga tatggtaaat acctcaacat
     6061 tcatgcagga agtcgcttaa tgtttggagc tttcgtttgc cctgtttcac ttgttctacc
     6121 tcctctaagg cattttttat gccgtgaaga acttcaagtg cgcgtttcat ttcatcaaac
     6181 tctttttgaa acaagttcca atccgcaatc ggcacctgca ctgcaacagg gcttccggcg
     6241 tcatcggtta cataatgaat agctgacatt gtcataatat tgaggtgtat tagagatata
     6301 cagtaaaata aacgattgga gctttatttg gtttcgtagc gcctgctttg tatatacctt
     6361 gcgccgccgg gttttgtgca tagcctttgg cacaagttat tgattagcag cgcccggtat
     6421 ctcacctgac ggtgcgaggt ctatatactc ggttcagggc aggatgttac acttcctccg
     6481 gaatgctctc aataaaatcc agaatagcgc cgccgccgag gtgtttggcc gccgaagtaa
     6541 cgaactcctg cggcagtaca ttccagtgtt cgagcagggc ttcgcgaagg cgggtaatgt
     6601 tttgctcgcg ttgagcatcc gtcaggcggt cggttttggt ataaacaatg acgaagggca
     6661 accctttttc gcccagccag ttaataaatt ctatatcaat gttttgtggc gggatattgg
     6721 aatcgagcaa tacgaaagta cacaccagat tggcacggcg actgaggtag ccctgaatca
     6781 taatttccca cttgcggcga ttggtttgcg aggtgcgcgc atagccgtag ccgggcaggt
     6841 ccaccagata ccagtctttg ttgatgagaa aaaaattgat ggtttgggtt ttgccgggct
     6901 tgccggaggt gagggccagg tctctgcgcc ccgtgatgag attgatgagc gaagacttgc
     6961 ccacgttgga gcgcccgatg aaagcaaatt cccgacggtt gtctttcggg caggtcagtt
     7021 cagatgggta gctgccggtg tattctgcgg agtggatggt catgttgaat cttgtttttc
     7081 tggctgcaag ccgttagcgg caagctgcaa gccttaattt tacttcgtaa aaggttttga
     7141 gtcgtgtttt gttttccaaa cgaaaaacct cctcccacag ttcacccagc ttgaggcttg
     7201 tggcctgtag cttgcggcca tgcacaatct ccgaatagaa gtttcacctc ccaaccctcg
     7261 cctgaatgcc tttcgcaatc gtattcaaca acaacaacaa ggtagtaaac acagcgatgc
     7321 gcgcaatgag atcgccccaa cgcacataaa acgtgacgcg gtcattgagg cgcacctgac
     7381 ctttgatggc aatggcctcg ttgtagcggg tggcttgcag gatgtcgccg cgctgattga
     7441 tgaaggccga gatgcccgtg ttggcagcgc gggctatgct gcggcgggtc tcgacggcgc
     7501 gcaaagtgcc cagccagagg tgctgccggt ggccgggcga gttgtcccac cagccgtcat
     7561 tggtcataat gacggcagcc tgcgcccctt tgtggatgta gcccgtgaag aactcgccaa
     7621 aaatggattc gtaacaaatc accggcgcta agcgggcctt atcgctgctc atggccatgc
     7681 gttctttttg cgtgccgacg ccctctacag tgccctgcac ctgtttgatg acgggttcaa
     7741 aaacgccgaa aaaccgacga tacggcagga tttcaggccc cggcacgagc ttcgacttgc
     7801 ggtacaattg tacctcctcg gattcgttgg aaaactgcgc cgccgcgttc agcacctcaa
     7861 aatacatggt gccgccgccg ctgcgattcg aactgcgaac agccttggaa tgcggctcgc
     7921 cgggcagaaa aatatggtac gcatctatac cggcaatgac tttgagttgc gggtgctttt
     7981 ccatgaagcc gcgcaactgc accactgccg ggtagctctc cactgagcgg gtttctacat
     8041 aaccgaaact cgtttccggc cacaccaggt agtcggtttt gctgtcggtg acggaggccg
     8101 acaattccag aaaacgctgc atctgcacgt cttccgggat gtcgaatttt tcgtagtgcg
     8161 gctcgtaatt tggttgtacc aatgctacct gcaccgtttt atcgccctct tcctgataag
     8221 tgaaatattg ccaaagcgaa atgccggtgg gaacgagtat ccaaagcgcc gttttcacca
     8281 cagacgaacg aagcgccgcc cggtcatttc tctgtttcca ggcattgaaa atcaaccagt
     8341 taccgactaa aatccaaagt cctccaccga aaacgccggt aaattcatac cattgtacca
     8401 atgccggaaa ttgcgcgaaa gcgttgccca atgtgagcca gggccaggtg atttcccagt
     8461 tgagatgcag gtattcaaat gccatccagt aggccatgaa ggcagctccg gcaatgcgcg
     8521 gcatcctgcg ttgggtatga tgaaaagcgg tgnnnnnnnn nnnnnnngcc atgaacaggc
     8581 tgttgagcgc catggccaca atgcccgccg ccagcgccgt gttggccacc caccaggtgc
     8641 tgaggatgtt ccagatcaaa aaagcattgt agctgtgcag caaaacctgc ttgcctttgc
     8701 cttttgcatc gctgccgctg atctcagatt cgagcagcag caaaggcaca aagcccacga
     8761 acatcaacaa aggcgcaggt acgatgcccg gaaaacccag tcccaacagc agtccgctga
     8821 gacttgaaag tcccgccctt cgccaaccgg cagctccgtt tttcctgccg cacagcagag
     8881 cctgcgccag tatccacaaa gcgatgaaaa gcagcagcgg gcgatgcccc cacagcaatc
     8941 gccggtgcgt tggtacatgg agtatcccgc ccaggcagcg ccgagaatgg aaataataat
     9001 ggtgagtatc cggaggcggc cactcattta cgcgtggttg gcttgcaggt gtttggccac
     9061 ctgcttttcg gcccattggg tggtgacgga tttggggcgc tccagcggga agcccaatgc
     9121 ccgcgaccag gccaattgag ccagtacgcc caatgccctc gacacgccaa acagcaccgt
     9181 atagaagttg aattccgtga gaccgtagtg aatgagcagc gcgccggagt gagcgtccac
     9241 gttgggccag gggtttttca ccttgccgag gctttccaaa atgggcggca cggtttcaaa
     9301 cacatcccaa accacctgca ccatgtcgtc gtgcttgatg tagcgttccg cgaagctctt
     9361 ttgcgccatg aagcgcgggt cgggcttgcg caacaccgca tggccatagc ccggaatgac
     9421 ctgaccggcg tcaatggtgg cctttacata ggctgcaatt tgatctttgg taggcgtttt
     9481 ggtgcccagc gcatccagca ttttgtaaat ccaacccact acttcctgat tggccaaacc
     9541 gtgcaacggc cctgccagcg aagtcatggc gccggcaaag gccagatacg catcgctcag
     9601 ggtggagcct acgaggtggc aggtatgtgc cgaagcgtta ccgccttcgt ggtcggcgtg
     9661 gatggtgagg tacaaacgca tgaggctgcg aaaatcgggc tgatcgaagc cgagcatgtg
     9721 ggcaaaattg ccgccccagt ctttgctgat atcgggtgca atatgatcgc caccgtggaa
     9781 agaacgccga tagatgtagg cggccacgcg cggcagacga gcgatgaggt tcatcacgtc
     9841 ttcgtaggtg gcgtcccagt attcttcttt gtgcaggacc tcttcgtgcc ttctggcaaa
     9901 acaactctcg gattgcatgg ccgtcaccgc aatgatgaac tgcgtcatgg ggtgcgtgct
     9961 gatgggcagg gcatccagcg tgtggaaaac atgttccggc acattgctgc ggtgcgtcca
    10021 ttgcagcgtg agccattcca cctgttcttc ggtaggaatt tcgccggtga gcatgagcca
    10081 gaacagccct tcggggcgcg gttccttttt gtcttgccct ttgggtagta tttctctgag
    10141 ttcgggtatg gaataacctc tgaatttgat gccttcttcg gcgtcgaggt cggaggtttc
    10201 ccaaaccatc atttttatgc tgcgggcgcc gccgtaaatc tggccgagcg ttacctgatc
    10261 taccacaagt tcgccgtgtt ctttgagcat ggctctgatg gcattgcgtt gagcagtggc
    10321 tttttcgtgg aataattgct tcaagggatc cattgaaaaa tgctttattt ggtggtaaga
    10381 tgaatgttaa aaaacgctcc gggcgacagc cggcggcagc cccgaaaaac gggcagcaat
    10441 attacattac taccttagaa ttgtttggct aaacagcaaa aatcggcaaa aaaaagcctc
    10501 cccgaccgta agcgccggtt gttgggtaat gactgccgaa ctcaggtttc agcaacatat
    10561 tgactaatgg tcggattcca tttccaaaag agcctcatgg atggcatctt tggatgcgcc
    10621 gctctttttg agggcttcta ttcgggcggc ttcgctgcgt cggcgctcgg cccgctcccg
    10681 gtctaagatg ggcttctgtt cgggcgtgag catgttggca atcgaggatt cggtgagggt
    10741 gcgaatgctc ttcagctttt ggcgatagag cgattcgtcg gtggttttca gcgattcgat
    10801 ttcggacagg ttgcggaagc ggcgttcctg aatcacatac attttttgca cctgcgcctc
    10861 actgaggctg taaacggcgg tcaattcttc cgtagctttg cgggccgatt gctggtcggc
    10921 ggcagattgt gcgaaagcgc tgcaaacgct caaaaaggca atcataccaa tcaaaaattt
    10981 cttcatcttg agttgttttt tacattgccg gccaaaatac ggctttttga agtatgtaca
    11041 agtaatgtgc aggattttcc gttgaaattg cctgcctgaa cacaaaccga ccgaccattg
    11101 aacatcctcc caaaactact gctcaccatt gttttgctgc tttccgttgc cccggcgctc
    11161 ctcgcccaaa aagcggcgta ccggcactat taccggggtg ctatgttgta tgccgagggg
    11221 cagttccggc aagcggaggg ccacttccgg caggccgccg agatcatacc cgacaatttt
    11281 tactttacct cggcctatgc cctcgcactg agccgcagcg ggcagtacga atcgggcagg
    11341 ctgctgctgc aaaaggccgg cggattcatt gcgctcaaag accccgagcg cgtttccaaa
    11401 ttagcgtcct ggcacttttt tcagggcatg tctcatttgt acggaggccg ggcgggctat
    11461 gcggcggcgc ccatccgcaa ggccatcgag ttgatgcagg ccaagcccga tggccgtcaa
    11521 ctcagcattt tgtacaacgc cctgggctat gccacgctcc tgcatcaggg gcaaggctcg
    11581 catgtgaagg cgggcattgc gcctcatttt catgtgcaac gccgcagtat ggagcgggcg
    11641 gctcaatatt ttgaagaagc tttgcgccac gacgcaacca atgccgtagc cctgcaaaac
    11701 tatcgcacac tggccgatac gctgggcctg ccgccggggc agttctccgc tcaggccgac
    11761 tctctgaacc aacgccgcct gctcgacccc attttcagtt ccatgcctgc caacattgac
    11821 cgaacgctgc aattggggca atttgaggag gtgctgtttc tggtggatat ttccggttcg
    11881 atggtcatgg aaaaggtgat ttgcctcaac caggaccgct ttgatgtgat gaaagaaatg
    11941 gccctttatc tcaccgacaa aatgcccgaa accnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnncgg agctactgag cgcgcaggat tcggtttcaa
    12181 aatccatttt tctcatcagc gacggggaaa acgtatgcaa aatgggcgac ctcgacatct
    12241 gcgaatgggc tgccggtctg gccggaaaaa acatcaccat tcatgtgctc actttcctgg
    12301 agtcggggct gcacaacacg ggcgctttcg cagaatacac ctgcctggcg gaaaacaccg
    12361 gcggcaaagt ggtctatatt gacaactacc gctgcaccac ccaactcttc gacttcaacc
    12421 tgttggaaac ctgtaactta gccattccgc cgctgcaacg ctcgcaatgc tggggggtgg
    12481 cggtgaagga tttgtgggct
//
LOCUS       DAORLF010000082        12471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2696_length_12471_cov_1.683151, whole genome shotgun sequence.
ACCESSION   DAORLF010000082 DAORLF010000000
VERSION     DAORLF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_12471_cov_1.683151"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>267
                     /locus_tag="PK198_08720"
     CDS             <1..>267
                     /locus_tag="PK198_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38859.1"
                     /translation="GNVAARKARRIALVSNSSWYTCNFRLGLLRRLRDMGAEVYVISP
                     HDHSSTRLIAEGFHFVQLPIAVYGTNPLREWRGILRLISIYRKYR"
     assembly_gap    268..295
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <296..>897
                     /locus_tag="PK198_08725"
     CDS             <296..>897
                     /locus_tag="PK198_08725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38860.1"
                     /translation="NIYGSMATWWCRIPSIAITTGLGLMREEGWSFSKWVVIQLYRLA
                     GLFSRQVWFLNRDDQQFFEQLRIVPADKSFLLPSEGVDVQWYKPGHERREHKPLHFLY
                     AGRIVWSKGMKEFYEAALHFHRKGAPCSFHLVGFIVPDHPDAVSFDLVQEWQQQGIVR
                     FHGETDDIRPFLAEADCVLLPSFFGEGVPRILLEAAAMAK"
     assembly_gap    898..992
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <993..1175
                     /locus_tag="PK198_08730"
     CDS             <993..1175
                     /locus_tag="PK198_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38861.1"
                     /translation="QTIETFLELSPSERRQMGWEGRKKVLREFEEEIVIRHYLHALTR
                     FLGWPAKRKKHKSVRG"
     assembly_gap    1266..1369
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1370..2110)
                     /locus_tag="PK198_08735"
     CDS             complement(<1370..2110)
                     /locus_tag="PK198_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38862.1"
                     /translation="MRRIAVIDLGTNTFHLLIVERTEDAPFRVVFRKQVYAKLGIGGV
                     EYIIPESFERGLETLEAFQMAIVEYGAELVQAFGTAALRTASNAPEFIEQALRRTGIP
                     ITLISGREEARLIHRGVSWAVPIEDDEPVLIMDIGGGSVEFIIADKNGPLWYQSFMVG
                     VAVLFRNFHHSDPITEDETEAVRSFLKQQLQPLLTALQQHPVRRLIGAAGAFDVVEGM
                     TLPEIPTSPNAEIPLERFPEVYQRIIAAT"
     gene            complement(2126..2599)
                     /locus_tag="PK198_08740"
     CDS             complement(2126..2599)
                     /locus_tag="PK198_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38863.1"
                     /translation="MKYSTIPFLLLVLFSACSSQSGSDAAASSKQAAPAPAFPTLPIE
                     TIRTLVAECDYIDIIMYQPQFSMSQNERSAVMATLSHISEVPAVHNAANPATGHIFYQ
                     AKGQTLLEADLYLRNEGSYLVFYQDGKPAFANAMTQQGVAFFANILSQLQGAPQQ"
     gene            complement(2633..3241)
                     /locus_tag="PK198_08745"
     CDS             complement(2633..3241)
                     /locus_tag="PK198_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF38864.1"
                     /translation="MRYSLILFVLMTVFCLAAFSVQAQRFKTAVVAGLNMAQIDGDDL
                     AGYRQPGINAGLRTSAQLSDKWQLGLELLFSQQGSDRGPDDPFLTNYDRIRFNMVEVP
                     AMIQFSEWKIQLAAGLSYSRLINYRIRGIGGSNIASNFDIDRNNVSVLLGGTYFPSEK
                     WGLDVRWTRAMFPLQVYSSLSGAETDQFLSYFVSFRLLFLLN"
     gene            3281..3619
                     /locus_tag="PK198_08750"
     CDS             3281..3619
                     /locus_tag="PK198_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38865.1"
                     /translation="MHYLCARQNTISGMLKYTAHNLKKIETLLEELGYTVRYEKGNFQ
                     SGYCVVEDRKMAVVNKFFNTEGRITCLLDIIAGIEAGDAGMSEKSAKLYKEILQSSSS
                     EPAQSETPSN"
     gene            3733..4383
                     /locus_tag="PK198_08755"
     CDS             3733..4383
                     /locus_tag="PK198_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF38866.1"
                     /translation="MSIDNQNIAIDCGPDFRQQMLRADVSSLDAILITHEHNDHVAGM
                     DDVRPFNFFQRRDMPVYATVRVAAELRRRFAYAFGPDAYPGAPRLLLQEIQKDTPIQI
                     GSTSIVPIEVMHGSLPVLGFRVGSFTYLTDVKTISEAELEKVMGTEILIINALYHTPH
                     PTHLHLEGALELIERIGPRKAYLTHIAHSMGRRCDVSPALPPGVELGWDGLEIVVD"
     gene            complement(4603..>5268)
                     /locus_tag="PK198_08760"
     CDS             complement(4603..>5268)
                     /locus_tag="PK198_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38867.1"
                     /translation="SLSSNFIRVFNAVADGGTVSTTDGDTQVELDMQDGEADVLEFEN
                     TGATNLPYLYVLTDENNIVLGYLNGNSFDFEGRTEPKLRVWGLSYHGAAPTATDVPAS
                     ELIGAGDCAAFSQNFVSIAAIVDPTLQDNATSLRATAQPNPATDRVTLRVWADEKVKS
                     ASATVLIMDASGRVRDQYRLPEWSGFQQAELDISRLEEGVYTVQIRSAENVAVLRVVK
                     SRM"
     assembly_gap    5269..5280
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5281..>5653)
                     /locus_tag="PK198_08765"
     CDS             complement(<5281..>5653)
                     /locus_tag="PK198_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38868.1"
                     /translation="NILSVDLGSSCLELSGNFITVIVTDIIAGGVLSANGDLTTVYFC
                     PNDGQEDLVEVSTTSPTPSDNYRFVVANQNGMVFIPDLMGNAIDFDGAGIGEYRIYGI
                     AFTGNYLVGLGTNLNTADLSDG"
     assembly_gap    5654..5670
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5671..>7016)
                     /locus_tag="PK198_08770"
     CDS             complement(<5671..>7016)
                     /locus_tag="PK198_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38869.1"
                     /translation="ALLVQVGDNAAEVDLASECSELSNFLTVLRQKPEAASISLSDGN
                     TFISACADGLTGPVSFINTSDFAGNYIYVITSTSNVILDVAAGSSYDFSDLTNDTVRV
                     WGLAYTGNILAQIGDNAATTTITDDCYDLSDNFITVVRVNAVGGSLITPDGQTQYLRC
                     AGDGTPDPISFTVTGASNAPYLFLITDENDYLIGISTQPGFDFENAIQGVFRIRGISY
                     TGVFQLFPGDSIFGDIPAASGCFDFSDNFIEINNVRVNGGNLFSTGNQTVFYICPADG
                     VADVVHFNNTSNAATANYAYLFTNENNVIIGLTFQDSFNFDIAGIGITRIWGVSYTGN
                     FIANFGDNAATTPLSDQCYDLSNNYITVVRDVPAGGTVATSQGETALTICLGIDPSVV
                     TAQSTSASLVAYTFALTTQFNDILAFSDGGNFNFAQYGAGTYRIWGVSYTGTLDLT"
     assembly_gap    7017..7069
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7070..>7947)
                     /locus_tag="PK198_08775"
     CDS             complement(<7070..>7947)
                     /locus_tag="PK198_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38870.1"
                     /translation="VDGGTVSLIDGGGNAYLACANDGSADVALVQSTANEPGANYTWL
                     LTDEGNQILAISSTADFNFDNYDPGTYRIWGLGYTGSLSAAPGQFAGTVSLASECFGL
                     SLNYIEVSVETALGGEISVADGSHFISICAGNDIVSEPITFNTTGSSTGTYVYVITDE
                     ENNIVRVYNDDVISFDDGLGEGRYRVWGLAYTGDLTAQIGDNAGDVSLSDGCFQLSSN
                     FIPLWSGFPEAGELSFEGSGDTRLICPDNGIADEISFNAAGLSQNLPSVFLLTDADNV
                     VLDIFTDTTYDFDQTD"
     assembly_gap    7948..8009
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8010..>9897)
                     /locus_tag="PK198_08780"
     CDS             complement(<8010..>9897)
                     /locus_tag="PK198_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38871.1"
                     /translation="ACVNNSGSNAISFATTNTGSNYSYVVTNANNIIVGAATGNMFDF
                     GTVPAGNYRVWGVAHAGPLNAPIGANITTSVLSNQCYGLSSNFVTVQRGNADGGTVST
                     VSGASEIKFCRAADAVAVALTVQSPAPASYAYVLTNTANRIIAILPGNSFQPAGLPLG
                     NYRIWGLSYSGNILAQAGDDAAAVALSDACFDLSDNFIPVALQDVNGGEISIAGGGNQ
                     ALCVGDGDADVVSFSAAGGSGASYIWVVTDADGEQVLAVSTSNDIDFEGFASGSVLVW
                     GLAYSGTLNAQPGQSVAEALQGLECFDLSENAVALHLTRTNGGEVTIAGGATSINVCT
                     GDGVSDELSFTNTSDASENYAYVVTDGNNNIIALSASGVVDFEGGGVGVVRVWGLSYS
                     GNLLAQVGDNAASTTLSDGCYDLSDNFVTVDLRRTNGGNVSITGGAVFATVCANDGAA
                     DVLSFNNNSGFAPNYAYIVTNENNVVQSISTTGMIDFEGSGSGIVRVWGVAYTGNLLV
                     APGDNAAAVALSDECFDLSDNYVVVDRETVDGGMIMTPFGQTQLEFCGGDGFPDQAVY
                     FSTMQAFGNFTFLITDANNTLLVQTTSNFTDFESFGAGTFRVWGLSYTGNATLQPGDN
                     VSSAT"
     assembly_gap    9898..10041
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10042..10348)
                     /locus_tag="PK198_08785"
     CDS             complement(<10042..10348)
                     /locus_tag="PK198_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38872.1"
                     /translation="MILKKVLPLVWLLLAAWSNSTAQSGSGCIEGAIVTTRAGATSVD
                     ICQGDGLPDIIRFKTSPAAMPFAYLITDVNNVILGVSASNIINLEGYPVGTLRVWAFA
                     "
     assembly_gap    10756..10782
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10783..>12077
                     /locus_tag="PK198_08790"
     CDS             <10783..>12077
                     /locus_tag="PK198_08790"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF38873.1"
                     /translation="PLAVSGQYSANQLVRDIFARGICNSISNISTIGPATGIGYFENG
                     LSSIGLDKGIILSTGPITNAPGPNNLTDKSGDFNYNGGDPDLNLLANTGVRDRAGLEF
                     DFVPLDSFVTFRYVFASEEYCEFVGSDYNDVFGFFISGPGIQGAFSNNSRNIALIPGS
                     NDFVAINTVNHLQNAGYFIRNELPADALQCGITPQSSPFTPLIQYDGFTRKLTASLKL
                     IPCETYHIRMVVADVGDNFYDSAVFLEAESFNIGAAVAIGGQSQSNASGIAYEGCNDG
                     HFRFQRADPSSRDLPVTVRYSIIAAASTATEGVDFAPLPGIATIPAGQSGVNVPVSFF
                     NDQILEGIEALIELNIKNPNSMGFSIYPSEFDIIFSGMNLGKAKLKKRVHIDANCEKA
                     YTFELNSSFKEFNFMDITKMLSGSKLGTMQVKGDLKAGK"
     assembly_gap    12078..12104
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12238..>12471
                     /locus_tag="PK198_08795"
     CDS             12238..>12471
                     /locus_tag="PK198_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRF38874.1"
                     /translation="MTDSELISAISKNDVSAKKQFYETYYPVLSGISIRYAKNKAQSN
                     ELLQNGMAHLLSHISNFKSNTNLILSDWVRNEFI"
BASE COUNT         2866 a         3070 c         3215 g         2751 t
ORIGIN      
        1 aggaaatgtc gccgcccgca aagcccggcg catcgcactg gtttccaatt catcctggta
       61 cacctgtaat ttccgtttgg gcctgctccg ccgcctccgc gacatgggcg ccgaggtgta
      121 tgtcatcagc ccgcacgacc actccagtac ccggctcatc gccgaagggt ttcacttcgt
      181 ccagcttccc attgccgtgt atggcaccaa ccccctgcgc gagtggcgcg gcatccttcg
      241 cctcatttcc atctaccgca aataccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaat
      301 atttacggct ctatggcgac ctggtggtgc cgcattccgt ccatcgccat caccactggg
      361 ctggggctga tgcgcgaaga gggctggagt ttttccaagt gggtcgtcat acaactgtac
      421 cgtttggcgg gtctgttcag tcgtcaggtt tggtttctca accgggacga ccagcaattt
      481 tttgaacaac ttcggatcgt gcctgccgat aagtccttcc tgttgcccag cgaaggcgtg
      541 gacgtgcaat ggtacaaacc cggccacgaa cgccgcgaac acaagccctt gcactttctc
      601 tatgcgggca ggattgtatg gagcaaaggg atgaaagagt tttacgaagc ggcactgcat
      661 tttcatcgca aaggcgcgcc ctgtagcttt cacctcgtgg ggttcatcgt tcccgatcat
      721 cccgacgccg tctctttcga tctggtgcag gagtggcagc agcagggcat cgtgcgtttt
      781 catggcgaaa cggatgacat acggccattt ttggcggaag ccgattgtgt gcttttacct
      841 tccttttttg gagagggggt tccgcgcatt ttgctcgaag ctgcggccat ggccaaannn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaaaccat cgagactttt ctggaactgt
     1021 cgccttccga acgtcgccaa atgggctggg agggccgtaa aaaagtactg cgcgagttcg
     1081 aagaagaaat cgtcatacgt cattatctgc acgccctcac gcgttttctg ggctggccgg
     1141 ccaagcggaa aaaacataaa tctgtgcggg ggtaactttt ttaataccat tacctactcc
     1201 ccaaaacact cccgaatcat accctccttg agggaaaaat gcgaaaccgt catgcgttga
     1261 ataccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtggcggcga
     1381 tgatgcgttg gtaaacttcc gggaagcgct ccagcgggat ttcggcattg ggcgaggtcg
     1441 gtatttcggg cagggtcatg ccttctacca cgtcaaaagc gccggcagcg ccaatgagcc
     1501 ttcgaaccgg atgctgttgc aaagcggtca ggagcggttg caattgttgt tttaaaaaag
     1561 acctgacagc ctccgtttca tcctcggtga tgggatcgct gtggtggaaa ttgcgaaaga
     1621 gtacggccac gcccaccata aaactttggt accaaagcgg cccgtttttg tcggcaatga
     1681 tgaattctac gctgccgccg ccgatgtcca tgatgagtac cggctcgtcg tcttcgatgg
     1741 ggacggccca actgacgccg cgatggatga ggcgcgcttc ttcccggccg ctgatgaggg
     1801 tgatgggaat gcccgtgcgc cggagtgcct gctctatgaa ttccggggcg ttggaggcgg
     1861 tgcggagcgc agcagtgcca aatgcctgca ccaactcggc gccatattca acaatggcca
     1921 tttgaaaagc ctctaaggtt tccaatccgc gctcaaacga ttcgggtatg atgtactcca
     1981 cgccgccgat gccgagcttg gcatacacct gttttcggaa aaccacgcgg aacggcgcat
     2041 cctctgtccg ctcgacaatg agcaggtgga aagtattggt tcccaggtca ataacagcaa
     2101 tgcgtctcat cagaaaagtg cggttttatt gctgcggagc gccctgcaat tggctgagaa
     2161 tgttggcaaa aaaagctacg ccctgttgtg tcatggcatt ggcgaaggcg ggtttgccgt
     2221 cctgatagaa gaccaaatag ctgccttcat tgcgcaggta gaggtcggct tcgaggagtg
     2281 tctgaccttt ggcctgataa aagatgtggc cggtggcggg attggcggca ttgtgtactg
     2341 cgggcacttc agagatgtgc gaaagagtag ccatgacggc actgcgctcg ttttggctca
     2401 tgctgaattg cggctggtac atgatgatgt caatgtaatc gcactcggct accagcgtcc
     2461 tgatggtttc gatgggcagg gtagggaagg cgggggcggg cgctgcctgt ttggaacttg
     2521 cggcggcgtc ggagcctgat tgggaggagc aggccgaaaa cagcacgagg agcaggaaag
     2581 ggatggttga atatttcata agtggttgat ttttattgta caacgaaaat ggtcaattca
     2641 gtaagaacag caggcgaaag gagacgaaat aactgaggaa ttgatctgtt tcggcgcctg
     2701 aaagtgagga atacacctga agaggaaaca tagcgcgggt ccagcgcaca tccaagcccc
     2761 atttttcgga ggggaaataa gtgccgccca gcaacacgct gacgttgttg cgatctatgt
     2821 cgaaattgga agcaatgttg ctcccgccga tgccccggat gcgataattg atcaaacgac
     2881 tgtaagaaag gcccgctgct aattgaattt tccattccga aaactgaatc atggcaggca
     2941 cttctaccat gttgaatcga attctgtcat agttggtcag aaaaggatca tcaggcccac
     3001 ggtctgagcc ttgttgtgaa aacaatagct caagccccaa ctgccattta tccgacaatt
     3061 gtgcagaggt tcgcagacct gcattgatgc ccggctggcg gtacccggcc aggtcgtcgc
     3121 cgtcaatttg agccatgttc agccctgcca ccacagcagt tttgaagcgt tgggcctgta
     3181 cggaaaaggc ggccaggcag aagaccgtca ttaagacaaa aaggatcaat gaatatcgca
     3241 tagcagggta atttgcgcaa aaatagaaaa atcgcggccc atgcactacc tttgtgcaag
     3301 acaaaacacc atttccggca tgttgaaata taccgcgcac aatctcaaaa agatagaaac
     3361 gctgctcgaa gagctgggct acacggtgcg ttatgaaaaa ggcaatttcc aatcgggcta
     3421 ctgtgtggtg gaagaccgca agatggccgt tgtgaacaag tttttcaata ccgaaggccg
     3481 catcacctgc ctgctcgaca tcattgccgg aatagaggcc ggcgatgcgg gcatgtcgga
     3541 aaaatcggct aagctgtaca aagaaattct ccagagttct tcctccgaac ccgctcaatc
     3601 cgagacgcca tccaactgaa agttacactc ctcggcaccg gcacctctca gggtacgccg
     3661 gtcattggtt gcggctgcga ggtgtgccgg tcttccgatc cgcgcgatca ccggctgcgc
     3721 acatcggcgc ttttgagtat tgataatcaa aacattgcga tagactgcgg ccctgatttt
     3781 cggcagcaaa tgctccgggc agatgtaagc tcattggatg ccattctcat cacccacgaa
     3841 cacaacgacc atgtggcggg tatggacgat gtgcggccgt tcaatttttt tcagcgccgc
     3901 gatatgcccg tgtatgccac ggtgcgcgtg gctgccgaac tgcgccgccg ctttgcctac
     3961 gcctttggcc ccgatgccta tcccggagcg ccgcgcttgc tattgcagga aattcaaaaa
     4021 gatacgccca ttcaaatcgg cagcacttcc atcgtaccca tcgaagtaat gcacggcagt
     4081 ctgccggtac tgggttttcg cgtaggctcg tttacctacc tgaccgatgt gaaaaccatc
     4141 agcgaggcgg aattggagaa agtgatggga acggaaatac tgattattaa tgccttgtat
     4201 catacgccac atcccacgca cctgcatttg gagggcgcgc tggaattgat tgagcgcata
     4261 gggccgcgca aggcttacct gacgcatata gcccacagca tggggcggcg ctgcgatgtg
     4321 tcgcctgctt taccgccggg ggtagaattg ggctgggacg ggctggaaat tgtggtagat
     4381 taggatgagg gtagaacctc gcgcgggcag gactatactt ctcccgtgat gaatgtgggc
     4441 gggtagaacc cgcatgatag tccgcacaag gtagaacctt gcgcgggcgg agaaattgtg
     4501 gtagattagg aaagcacaaa gggcgccgaa cacttccgca ttcgacgccc tttgaattga
     4561 atcattttag cgactcaggg aaagacccca agccattaac cactacatac ggctcttcac
     4621 cacccgcagt acagccacgt tttcggcgct gcgaatctgc acggtataaa caccttcctc
     4681 caagcggctg atgtccaact cagcttgttg gaaaccgctc cattccggca ggcgatactg
     4741 gtcgcgtacg cggcccgaag catccataat cagtacggta gcagaggccg acttgacttt
     4801 ttcatcagcc catacgcgca gggtcacgcg gtcggtagcc gggttgggct gggccgtagc
     4861 gcgcagagaa gtagcgttgt cttgcagcgt cggatctacg atggccgcga tggacacaaa
     4921 gttctgagag aatgctgcac aatcacctgc gccgatcaat tctgaagccg gcacatcggt
     4981 ggcagtgggc gctgcgccgt ggtaggaaag tccccacacg cgcagtttcg gctcggtacg
     5041 gccttcaaaa tcgaagctat tgccgttgag atagcccaaa acgatgttgt tctcatcggt
     5101 cagaacgtac aggtaaggca ggttggtcgc gccggtattt tcaaattcca acacatctgc
     5161 ctcaccgtcc tgcatgtcca attctacttg cgtatcgcca tcggtcgtac tcactgtgcc
     5221 gccgtcggct actgcgttga acacgcggat gaagttggac gacaaggann nnnnnnnnnn
     5281 ccatccgaaa gatcggcagt attgagattg gtgcccagcc ccaccaaata gttgccggtg
     5341 aacgcgatgc cgtagatgcg gtattctcca atgcctgcgc cgtcaaaatc aatagcattg
     5401 cccatcaggt cgggaatgaa aaccatgccg ttctgattgg ccaccacaaa gcgatagttg
     5461 tcggaaggcg tggggctggt ggtactcact tctaccaagt cttcctgtcc gtcgttgggg
     5521 cagaaataaa ccgtcgtcag gtcgccgttg gcagaaagta cgccgcctgc aatgatgtcg
     5581 gtaacgatga cggtgatgaa attgccagac agttcgaggc aactactgcc caaatccacg
     5641 ctgaggatat tttnnnnnnn nnnnnnnnnn ccgtaaggtc gagcgtgccc gtgtaagaaa
     5701 cgccccatat ccgataagtg ccggcgccgt attgcgcaaa gttgaagttg ccgccatccg
     5761 agaatgccag aatgtcgttg aactgcgtcg taagcgcaaa ggtataggcc accaaagacg
     5821 cgctggtgct ctgagccgtc actaccgaag gatcaatgcc gaggcaaatc gtgagtgctg
     5881 tttcgccctg cgaagtggct actgtgccgc cggcgggtac gtcgcgcacc acggtgatgt
     5941 agttgttcga cagatcatag cactgatcgg aaagcggcgt tgtagcggcg ttgtctccaa
     6001 aattggcaat gaaattaccg gtgtaagaaa caccccaaat ccgggtaatg ccgatgccgg
     6061 caatatcgaa gttgaacgaa tcctggaagg tgaggccaat gatgacgttg ttttcattgg
     6121 taaacaagta ggcgtagttg gcagtagctg cgttggacgt attgttgaaa tgcaccacgt
     6181 cggctacgcc gtcggccgga caaatgtaga acaccgtttg gttgccggtg ctgaacaggt
     6241 tgcctccatt gacgcgcaca ttgttgattt ctatgaagtt atccgagaaa tcgaaacaac
     6301 cgctggcagc cggaatatct ccgaaaatgc tgtcgccggg gaacaactgg aacacgccgg
     6361 tgtaagaaat cccgcgaatg cggaacactc cctgaatggc gttctcaaaa tcgaaaccag
     6421 gctgggtaga aatgccgatc aggtagtcgt tttcgtctgt aatgaggaac agatagggcg
     6481 cattggatgc tccggtgact gtgaaactga tcggatcggg cgtgccgtcg ccggcgcagc
     6541 gcaggtattg cgtttggcca tcgggagtaa tcaatgaacc gccgaccgca ttgacgcgta
     6601 ccaccgtgat gaaattgtcg gacagatcgt agcagtcgtc tgtaatggtc gtggtagctg
     6661 cgttgtcgcc aatctgggcc aggatattgc cggtgtaagc caatccccac acgcgcacag
     6721 tgtcgttggt caggtctgag aaatcgtagc tactgccggc ggctacatcc agaattacat
     6781 tgcttgtgct ggtaatgaca tagatgtagt tgcctgcaaa atcagaggta ttgatgaagg
     6841 aaacaggtcc cgtcagccca tcagcgcaag ctgaaataaa ggtatttcca tcagaaagac
     6901 tgatgcttgc ggcttccggc ttttggcgca acacggtgag gaagttgctc agttcggaac
     6961 attcagaagc caggtccacc tcggctgcgt tgtcgcccac ttgtaccagc aaagcgnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtctgtctgg
     7081 tcaaaatcgt aggtggtatc tgtaaagatg tcgagcacca cgttatcggc atcagtgagc
     7141 aggaaaacag agggcaggtt ttgagatagg cccgctgcat tgaagctgat ttcgtcggct
     7201 atgccgttgt cggggcagat aaggcgggta tcgcccgatc cttcaaaaga caactcgcct
     7261 gcttccggga aaccggacca aagcggaatg aagttggagg aaagctggaa acagccgtct
     7321 gaaagcgata catcgccggc attgtcgccg atttgtgcgg tcagatcgcc ggtgtaagcc
     7381 aagccccaaa cgcggtagcg cccttcgccc aagccgtcgt caaagctgat gacatcgtca
     7441 ttgtaaaccc gcacgatgtt gttttcttca tccgtgatga catacacata agtgcctgtg
     7501 cttgagcctg tagtgttgaa cgtgatgggt tccgacacga tgtcgttgcc ggcgcagatg
     7561 gagatgaaat ggctgccgtc tgcaaccgaa atttctcctc cgagcgctgt ttccacactg
     7621 acttctatat aattgagcga caagccgaaa cactcggatg ccaaagaaac ggtgccggca
     7681 aactgccccg gcgccgccga taagctaccc gtgtaaccca aaccccagat gcggtaggta
     7741 cccggatcat agttgtcgaa gttgaaatca gccgtagaag aaatggcgag aatttggttg
     7801 ccctcatcgg tgagcagcca ggtgtagttc gctccgggtt cgttggcagt gctttgtacc
     7861 agcgctacat ctgccgagcc gtcattggca caggccagat aagcattgcc gccgccgtca
     7921 ataagagaaa ccgttcctcc atccactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgtagcggaa gaaacgttgt cgccgggttg
     8041 cagcgttgcg ttgccggtgt aggaaagccc ccacacgcgg aaagtacccg ctccgaaact
     8101 ctcaaaatcg gtaaagttgg aggtggtttg taccaaaagg gtgttattgg catccgtgat
     8161 gaggaacgta aaattgccga atgcctgcat ggtagagaaa tacaccgctt gatcggggaa
     8221 gccgtcgccg ccgcagaatt ccaattgtgt ttggccgaaa ggcgtcataa tcatgcctcc
     8281 gtcaacggtt tctctgtcca ccactacata gttgtccgac aggtcgaagc attcgtccga
     8341 aagtgccaca gcggctgcgt tgtcaccggg agcaacgagg aggttgccgg tataagccac
     8401 tccccacacg cggacaatgc cgctgccgga gccttcaaaa tcaatcattc cggtagtgga
     8461 aatgctttgt actacattgt tttcattggt aacaatgtag gcgtagttgg gtgcaaagcc
     8521 ggagttgttg ttgaaactca gcacatcggc tgctccatca ttggcgcaca ccgtagcaaa
     8581 cactgcgcca cctgtaatgg acacattgcc gccattggtg cggcgcaggt ccacggtgac
     8641 gaaattgtcg gaaaggtcgt agcagccgtc cgacagcgtg gttgaagctg cgttgtctcc
     8701 cacttgagcc agcaggttgc cgctgtaaga cagaccccaa acgcgcacca cgcctactcc
     8761 gccgccttca aaatccacta cgccggaagc tgaaagcgca atgatattgt tgttgccgtc
     8821 cgtcactaca taggcgtagt tttcggaggc gtcggaagta ttggtgaagc tcaactcgtc
     8881 ggatacgccg tcgccggtgc aaacgttgat ggaagttgct ccgcctgcaa tggtcacctc
     8941 gccgccgttg gtacgggtaa ggtgcaaggc tacggcgttt tcagacaagt caaaacattc
     9001 cagaccttgc aatgcttccg caacgctctg tcccggctgg gcattcaagg ttcccgaata
     9061 agcgaggccc cataccaata cagagcctga tgcaaagcct tcaaaatcaa tgtcgttgct
     9121 ggtggaaacg gccaacactt gctcgccgtc tgcatccgtc actacccaaa tataggacgc
     9181 tccggaaccg cccgcagccg agaaagacac tacatcggca tcgccgtcgc ctacacaaag
     9241 cgcctgattg ccgcctccgg caatagaaat ttcaccgccg ttgacatctt gcaaggcgac
     9301 gggaatgaag ttgtcggaaa ggtcgaaaca ggcatcgctg agcgcaacgg cagcagcatc
     9361 gtcaccggct tgggccaaaa tattgccgct gtaagaaagc ccccaaatcc ggtaattgcc
     9421 caaaggcagg ccggcgggct ggaaactgtt gcccggcaga atcgcaatga tccggttggc
     9481 tgtattggtg agtacatacg cataacttgc cggggccggc gactgtacgg tgagcgccac
     9541 agcaaccgcg tcggcagcac ggcagaactt gatttcggaa gctccgctga cggtacttac
     9601 ggttccgccg tcggcatttc ctctttgcac agttacaaag ttgctcgaca agccatagca
     9661 ttggttggag agcacagagg tggtaatgtt agcgcctatg ggggcattca gcggccctgc
     9721 atgagccaca ccccacacgc ggtaattgcc ggcaggtacg gttccgaaat caaacatatt
     9781 accggttgcc gctcccacga tgatgttatt ggcattggta acgacgtagg aatagttgga
     9841 gcctgtattc gtagtagcaa aggaaatggc gttgctgcct gagttgttca cgcaggcnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn naagcaaaag cccatacacg caatgtgccc accggatagc
    10081 cttccaaatt gataatgttg gaagcgctga ctcccaaaat cacgttgttg acgtcggtga
    10141 tgagataggc aaaaggcatg gccgccgggc tggttttgaa tcggataatg tcgggcaggc
    10201 catcgccctg acatatatca acagaggtag cgccggcacg cgtggtgact attgcgcctt
    10261 cgatgcagcc ggaaccggac tgcgcggttg agttgctcca tgcggctaat aacagccaca
    10321 ccagcggaag tacttttttc aggatcatgg ataaaacgtt ttaaggttgg attgatgtgt
    10381 ggacataccc atcaagtgga gcgttggatg gttttcggga agaacggggg gaccgtcctg
    10441 tgtttacacc tgacatatat gctcatgtaa attgcagcag cgaaattaat attaattttc
    10501 tctatgtggc gctatctgcc ggtttttttt tattaagctc cattcatact gaaacggtat
    10561 gactgaattt gcctaccttt gaaccgaaac ctaaaggaag ccttagcagc attctgccat
    10621 tctctttcaa ccaattacaa catgatcgtg agttggcgta ttttggcatg gatttttttc
    10681 agcgctgtgg gcagttttca gcttacagac tactttttta tatccctgtc tcagcagtct
    10741 gcgccccacg aggagnnnnn nnnnnnnnnn nnnnnnnnnn nnccgcttgc cgtttccggg
    10801 caatacagcg ccaatcagtt ggtgcgcgat atctttgctc gcggcatctg caacagcatc
    10861 agcaatattt ctaccattgg ccctgcaacg ggcatcggct attttgaaaa tgggctgagt
    10921 tcaattggct tagacaaagg cattatttta tctaccggcc ccatcacaaa cgctccaggt
    10981 cccaacaacc tcaccgacaa aagcggcgac tttaattaca atggcggcga ccccgacctc
    11041 aacctgctgg ccaacacggg cgtgagagat cgggcgggct tggagttcga ctttgtgccg
    11101 ctggattcat tcgtcacatt ccggtatgtg tttgcctcgg aagagtattg tgaattcgtc
    11161 ggcagcgact acaacgacgt gttcggtttt ttcatcagtg gaccaggcat tcagggcgcg
    11221 ttttccaaca attcccgcaa catagccctg atccccggca gcaatgattt tgtggccatc
    11281 aataccgtca accatcttca aaatgcaggt tattttatcc gcaacgaact gcctgccgat
    11341 gcccttcagt gcggtatcac tcctcaaagc tcgcccttta ctcctctcat tcaatacgat
    11401 ggcttcaccc gcaaactcac ggcgagtctg aaactcattc cctgcgaaac ctaccacatt
    11461 cgtatggtgg tggccgatgt aggcgataat ttttacgatt cggcggtgtt tctcgaagcc
    11521 gaaagtttca atattggagc agcggtggcc atcggcgggc aatcgcagag caacgcttcg
    11581 ggcatcgcct acgaaggctg caatgatggg cattttcgtt ttcaacgggc cgatccttcc
    11641 tctcgcgatc tgcccgtcac ggtgcgttat tccatcatcg ccgctgcctc taccgctact
    11701 gaaggagttg attttgcgcc gctgcccggc attgccacca ttccggcggg gcagtcgggc
    11761 gtcaatgtgc ccgtttcttt tttcaacgat caaatactgg aaggcatcga agcattaatc
    11821 gagcttaaca ttaaaaaccc aaatagcatg ggtttctcca tttatccatc tgagtttgat
    11881 ataattttca gcgggatgaa tctggggaag gctaaattaa aaaagagggt acatatagat
    11941 gccaattgcg aaaaggccta cacatttgag cttaactcct cttttaagga gtttaacttc
    12001 atggatatta caaaaatgct aagtggttca aaattaggca ctatgcaggt taaaggcgac
    12061 ctaaaagccg ggaaaatnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaag aagttcccga
    12121 ttgattataa aacctgtgat tacaagatga gtagataatc taaaatctaa aattattcta
    12181 acaattccgt aacctttgtt aataagcccc cgtacaagta gctaaatccc ttgtactgtg
    12241 actgattctg aactcatatc ggctatctct aaaaatgatg tctctgccaa gaagcagttc
    12301 tatgaaacct attatccggt tttatcgggg atttcgattc gttacgcaaa aaataaagcg
    12361 cagagtaatg agcttttgca aaacggaatg gcacatttgc tctctcacat tagtaatttt
    12421 aaatcgaaca ctaatttaat tttaagtgat tgggttcgca atgagtttat t
//
LOCUS       DAORLF010000083        12446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2706_length_12446_cov_1.783230, whole genome shotgun sequence.
ACCESSION   DAORLF010000083 DAORLF010000000
VERSION     DAORLF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_12446_cov_1.783230"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PK198_08800"
     CDS             complement(<1..1331)
                     /locus_tag="PK198_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38875.1"
                     /translation="MRIFTIFLHCLPWWPVVGFLLLSGLLHGQNDIKRIRIDNNAMVY
                     IPQQDKIFIATPSTHANNGNSMCVIDPYFGTIDTCYFIGSEPTVMAASDDGAFIYVGL
                     RGAPMVIKFNVLTRQVDAEIYLGASLSSPFYAEDIEVLPGNPNSIVVALQNQWNSQHE
                     GVVVFDGTVQRPNTSQNHTGSNSIAFAANGRLFGYNNFTSEFGFRELVIDADGVSELA
                     VYTGLINRYFVTIEGHQNRIYATDGRVINVNAATPSLAGNMALDLGTEAVLEVSPDSN
                     IIYFIVSSTGTSYKFERFNKTTFSKISSENLTGITGKVKSLINWGTNGKLAFNTDKAV
                     VIMRNCTSSLTNPPTFLPSITGVCFGDLATLRASSGFEHYFWSNGATGQTMTTSQAGQ
                     YFFQVADSTGCLGSRSNNILVNIDYPPSTPWISGNSIVGICRGGSTTLNAGG"
     assembly_gap    1437..1593
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1594..1866)
                     /locus_tag="PK198_08805"
     CDS             complement(<1594..1866)
                     /locus_tag="PK198_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38876.1"
                     /translation="MSGNQWFLNGEPIPGATGRFYSASLSGFYSVQTTINGCSSPMSN
                     LYNHFVVSTSSLPENEPMTAFPNPAQDVVYLKMNDEANTTCEVFVYN"
     assembly_gap    1976..1989
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1990..>3119)
                     /locus_tag="PK198_08810"
     CDS             complement(<1990..>3119)
                     /locus_tag="PK198_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38877.1"
                     /translation="TTPGHTGSNSITFASNGQLFGYNNETTEYGFRRLIISGNGVSEL
                     AVYPGLVNNFSTEIESQGNRIYSSRGHVVDVSTPTPNLVGTFSNLGGFPVMEVSPDSN
                     YVYFITFSLSEPQYKFEKYNKTTFNKISSEPLTGISGGLKGLINWGIDGKLAFNTDKL
                     VVIMRNCTSLLTSPLQMPSNAGGCTGTEVALSAPEGFNSYFWSNGAVGQTIFVGQHGQ
                     YSVRASDSLGCLGPPSNIVSVQFDWPPSAPWISGSSLVRICQGGSVTLSTFGSGNDSY
                     MWSNGATGTSITVNQPGQYRVRAISPNGCVGNYSQPVTVELLPETVPPQPVIEVLGST
                     TFCSGDSVILSAPEGYHSYTWSNSNGARLITVKNTGTFTVRVG"
     assembly_gap    3120..3132
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3133..3644)
                     /locus_tag="PK198_08815"
     CDS             complement(<3133..3644)
                     /locus_tag="PK198_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38878.1"
                     /translation="MKRRRQTRNNSDVMQYIALLAWSLLFVPNGVFGQNDMKRVPIDN
                     NAMIYVPQQDRLFVTTPSGQATGNSLCVIDPYFGTIDTCYFIGSEPGVMAVSEDGTYM
                     YIGLSGAPRIVRFNLLTKAVDAQINLGSSGVTGSFYAEDIEVLPGSSTSFVVSRRNQG
                     FSPRHEGLAV"
     gene            complement(3883..>4080)
                     /locus_tag="PK198_08820"
     CDS             complement(3883..>4080)
                     /locus_tag="PK198_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38879.1"
                     /translation="VKKLLPVSLELTRQGTSVKAVVYTGLEQIYCRGDDNEYADYCGL
                     FRQVQRKEIVLKWNKEKGKLE"
     assembly_gap    4081..4253
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4254..4616)
                     /locus_tag="PK198_08825"
     CDS             complement(<4254..4616)
                     /locus_tag="PK198_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38880.1"
                     /translation="MKKSLLLLSIFACLGCNKAPATTSAAPAATEEAPLAAPTVEEDV
                     PQERRDVLYYYRRLKEPYRSGYELKENKGQWTSMSPDTEEPIAAVVDLKNGFIEIVDE
                     GTGGGEWTYRMALFRMADG"
     gene            complement(4639..>5270)
                     /locus_tag="PK198_08830"
     CDS             complement(4639..>5270)
                     /locus_tag="PK198_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF38881.1"
                     /translation="TNITSGLAIAAYERTILANQSPWQLWLRGQSNAMSETEKAGAIL
                     FFGKAQCANCHTGPALNNMNFHALGMKGLNECPEPVFKTPGNAPEHLGRGGFTGRAED
                     MHKFKTPQLYNMSDSPFFGHGASMRSIRQVVEYKNRALPENPNVPASQLDPYFVPLSL
                     SEQEIADITAFLTKSLHDPNLRRYQPIRVLSGQCFPFNDPLARAQLGCN"
     assembly_gap    5271..5438
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5439..5784)
                     /locus_tag="PK198_08835"
     CDS             complement(<5439..5784)
                     /locus_tag="PK198_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF38882.1"
                     /translation="MELGRLLFHETGFGLEPAQAIGQGRFSCASCHFASAGFQAGRFQ
                     GIGEGGIGFGLNGEGRERDINYIVNNMDVQPIRSPAALNTAYQELMLWNGQFGGVGMN
                     IGTEHAWTAGTPK"
     gene            complement(5756..5989)
                     /locus_tag="PK198_08840"
     CDS             complement(5756..5989)
                     /locus_tag="PK198_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38883.1"
                     /translation="MKRHYFVLVAALVLLMGACRPDDDTSALDQELDEVLQTASNGAG
                     RAFFRMPASDDYAAIPQKSHHQSQSGTGPPALP"
     gene            6106..6858
                     /locus_tag="PK198_08845"
     CDS             6106..6858
                     /locus_tag="PK198_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRF38884.1"
                     /translation="MERLFGLIGYPLSHSFSKRYFTEKFEREGIAGCRYELFPLERIE
                     DLLTILEENPSLCGLNVTIPYKQAVIPYLHQLDAGAQAIGAVNAIRIADGKLTGYNTD
                     AYGFMESLQRFLQQSGHQPPGALVFGTGGAAKAVVWALQQLGIPHLSVSRRAEAGSIL
                     YESIDKEHFTTHPLLINTTPLGMSPHTDACPPLPYDLLDRQNLLYDLVYNPEETLFLQ
                     KGKEAGCHTTNGLEMLYLQAEKAWSVWNDPAL"
     gene            6885..8138
                     /locus_tag="PK198_08850"
     CDS             6885..8138
                     /locus_tag="PK198_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRF38885.1"
                     /translation="MESSGKPVPKNVEKMLEAWQQSGDEASLPLPNFADTATAFAVKT
                     DQELEKAARLFGLMNHHWLVGMGSMLGLAAIRLRMPFVESVVKNTIFEQFCAGTTLLD
                     SAPNIQKLHGAGVYSALDYGVEAKETEEDFNHTMNEIIRAIEFSEQCPGIRLISVKIT
                     GLARFGLLEAVHNKGNLNPRERNEYKNVLKRIDAICHTADQKGISVFIDAEESWIQDA
                     IDHLATLMMARYNHERAVVYNTFQMYRTDRLQFLIRSYNRAAADGYFLGAKLVRGAYM
                     EKERDRAERLGYESPIHPNKEATDHAFNTGVRFCMDHYEKIAFCNASHNADSAMLQAE
                     LIRERQLPRQHPHLLFCQLYGMSDNLTFNLAKAGFNAGKYVVYGPVREVVPFLIRRAQ
                     ENTTITGDMSREHQLVTKELKRRGG"
     assembly_gap    8245..8308
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8309..8617)
                     /gene="queD"
                     /locus_tag="PK198_08855"
     CDS             complement(<8309..8617)
                     /gene="queD"
                     /locus_tag="PK198_08855"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRF38886.1"
                     /translation="MEIFKEFTFDSAHWLPYVPEDHKCRHLHGHTYIVKLYLRGELHP
                     VLGWVTDFADIKDAWRPLEKQLDHKLLNEIPGLENPTAENLAVWIWNHIQPTLPQLSK
                     V"
     assembly_gap    8857..8898
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8972..>9179)
                     /locus_tag="PK198_08860"
     CDS             complement(8972..>9179)
                     /locus_tag="PK198_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38887.1"
                     /translation="DADMRRPVAALRADGSFYDTPETDWLWEYEIRKHLKDKNIEYIS
                     VWSAAWWRDAKEEARKLAGAVLKA"
     assembly_gap    9180..9215
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9216..>11696)
                     /locus_tag="PK198_08865"
     CDS             complement(<9216..>11696)
                     /locus_tag="PK198_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38888.1"
                     /translation="IRNVQSDQAAVLGLLRSAVSAPPIVEAFPADRYKLLLEKALRLK
                     DKLDAGYHATRRPVFGTHNWTETVGLFLRSQSRAGKELLGQQLYNQDFAFTFEEYEVL
                     RRGIAQSQVLFPSINTLKHPLSALKGELFTQKDKDGGLAYIRQQIAAFSERSARLHHR
                     YISTLNGYSDKLNDLYQGSRQQLNEAIFRLKGMLTDYGNQWGADFENSSEGGLKLSRI
                     VSARAQQILTAREQVREAFAELERIWTARPLFDFAVLPEKDRKSMAKIRANVLEMEAS
                     FGLWNEQLPAQIQEEVQRLSSKSVHADLPYTTIVTELEQGLDSLLNDVNLAGILEQPL
                     EHQALTLTKRLKYLEEVMESFDNIGLYLKDYDTFYEWQRHWIGMPENGRKIVRALAKA
                     KPNDWTAALDSWYFNNCLTMQFSPDLPVSDEAVEELAETMAGLQPLLLSRLRTLWDER
                     REAVAKELRRGGRKTSQALSGTADAGVDLPGLFRESGRHITDIFPIVLAPPAVAQALF
                     NADTGFDVVIVDEAQAVPAAEAAPLLHLGRRALAVGDPAQQPERDDASLLGLFRAAGY
                     RILPLGIIHQLKPGNLLQSAWKVAVADEAPRGFELQLTHTHGFYYEHSGINEKEAEAA
                     LQLLNRIQPTPQRTMPSVGIVCATPQQRDLISYYLLRIKQRNEPGADVVQQLERNGLG
                     VFHLGELPGLHFDEMILSATFGPSAPNGKVSGHIEMLNGPDQTAQLAALMSRASTKVH
                     ALCSIDDIDLQRMAGDTSRRGECLLANYLLFLSAFRQSKAKEQRSIIERVAALVRKGE
                     HAQEVSVFHQEVAAVLTSYIGKDRMSPG"
     assembly_gap    11697..11711
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11712..11942)
                     /locus_tag="PK198_08870"
     CDS             complement(<11712..11942)
                     /locus_tag="PK198_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF38889.1"
                     /translation="MEGHPFSLLPLDPWQATAMQQVERSRITVAAGISGTGKTNLIAN
                     VIINALANGRKCLMVCSRLSELKEAQAVLGRCG"
BASE COUNT         2731 a         3249 c         3059 g         2725 t
ORIGIN      
        1 ccccccgcat ttaatgtagt gctgcctccc ctgcaaatac ccactattga attacccgaa
       61 atccaagggg tggacggcgg gtagtcaatg tttacaagaa tgttattaga acgagaccca
      121 agacagcctg ttgaatcagc tacctgaaaa aagtattgcc ccgcctggct ggtagtcata
      181 gtttggccag tagctccatt tgaccaaaaa tagtgttcga agccgctgga agccctcaat
      241 gtagccaaat ctccaaagca tacgccggta atactcggca gaaatgttgg cgggttggta
      301 agcgatgatg tacaatttct cataatgacc accgccttat cggtattgaa agccaatttg
      361 ccattggtcc cccaattaat caatgacttc acttttcccg ttattcctgt caggttttca
      421 gaactgattt tgctaaacgt ggtcttatta aatcgctcga atttgtaaga ggtcccggtt
      481 gatgaaacga tgaagtaaat aatattagag tccggagaga cttccagaac ggcttcagtg
      541 cctaaatcca gtgccatgtt acccgctaaa gaaggcgtag cggcatttac attgattacc
      601 ctgccatcgg tagcatatat tcgattctga tgtccttcta tagtaacgaa atatctattt
      661 atcaagcctg tatatacggc gagttcagaa accccgtctg catcaataac caattcccga
      721 aagccaaact cagaagtgaa attattgtac ccaaataagc ggccgttggc ggcaaatgct
      781 atcgaattac tgcctgtatg attttgagat gtattggggc gttgtacagt gccatcaaag
      841 actaccacgc cttcatgctg gctattccat tgattctgta gagctacaac aatcgaattg
      901 ggattacccg gcaacacttc aatatcctct gcataaaaag ggctggataa acttgccccc
      961 aagtaaatct cggcatctac ctgcctcgtt agaacattaa acttaatcac catcggagca
     1021 cctcgcaacc ccacataaat aaaggcgcca tcatcagacg cagccatcac tgttggttca
     1081 cttccaataa aatagcaggt atctattgtt ccaaaatatg gatcaatgac gcacatgctg
     1141 ttgccgttat tggcatgcgt actcggcgtt gctatgaata ttttatcctg ctgtggtata
     1201 taaaccatcg cattattatc tatacgaatg cgctttatat cattttgccc atgcaacaag
     1261 cccgataaca ataaaaaacc aacaacgggc caccacggca ggcagtgcaa aaagatcgta
     1321 aatattctca tggttttcag attgacaaaa tgaatcctgt acatcactta ttgaaccggc
     1381 tccggcacag tgccggctcc gggcagcagg ccattcgggg ggaacaatta tattttnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattatag acaaacacct cgcatgttgt
     1621 attagcttcg tcattcatct tcaaatagac tacatcctga gccgggttgg gaaacgccgt
     1681 cataggctcg ttttcaggca acgaagaagt acttactaca aaatggttgt acaaattcga
     1741 catcggggag gagcagccgt tgatcgttgt ctgcacagag taaaagccgc tgagacttgc
     1801 cgaataaaaa cggcctgttg ccccaggtat cggctctcca ttcagaaacc attggtttcc
     1861 agacacggca gaagatgcaa gaagatttcc gttggccaga attgccggtt gcgcaggaac
     1921 cgggaataca gtgaccgata cgggaactga agtttcgctc atgcacccta atggcnnnnn
     1981 nnnnnnnnnc ccacccgaac agtaaatgtg ccggtatttt taacagtaat taaacgcgca
     2041 ccgttgctat ttgaccatgt ataggaatga tacccctcag gggcggataa aatcacacta
     2101 tcgcccgaac aaaatgtagt gctgcccaat acttcgatta caggttgagg cggcactgtt
     2161 tcaggtaaca attccactgt tacgggttgg gaataattcc ccacgcatcc attgggtgat
     2221 attgcccgga cacgatattg ccccggttga ttcaccgtta tgcttgtacc cgttgctcca
     2281 ttcgaccaca tgtacgaatc attaccagac ccaaacgtac tcaaagtaac gctgccgccc
     2341 tggcatatcc gaactaaaga actcccggaa atccacggcg cggaaggagg ccaatcaaat
     2401 tgcacagaaa ctatattgga tggtggtccg aggcatccta aagaatccga ggcccgaact
     2461 gaatattgcc catgctgacc aacaaaaatt gtttggccta cggctccatt cgaccaaaaa
     2521 tacgagttaa atccttcggg ggcgctcaat gcaacctcag ttcccgtgca tccgcctgca
     2581 ttggagggca tttgcaaggg tgacgttaat aatgaggtac aatttctcat aatgaccacc
     2641 agcttatctg tattaaaagc caatttgcca tctatacccc aattgatcaa tccttttaac
     2701 ccaccgctga ttccggtcaa tggctcggag ctgattttat taaaagtagt tttgttgtac
     2761 ttttcaaatt tgtactgagg ttcgctcaaa gagaatgtta taaaatatac atagttagaa
     2821 tctggtgaaa cttccatcac aggaaaaccg ccaagatttg aaaatgtgcc taccagattt
     2881 ggtgtgggcg tactcacatc cactacatgc ccccgactgg aataaattct attgccctgg
     2941 ctttcaattt cagtcgaaaa gttgtttacc aagccgggat atacagctaa ctctgaaacc
     3001 ccgttcccac tgattatcaa tcttctgaaa ccgtactcag tggtctcgtt gttataacca
     3061 aacaactggc cgttggatgc aaatgttatg ctattgctgc cagtatgacc aggcgtagtn
     3121 nnnnnnnnnn nntagaccgc aagtccttca tgacgagggc taaatccttg attacgcctg
     3181 gacactacaa aagaagtgga agaccccggc aatacttcaa tatcctccgc ataaaaagaa
     3241 cccgtcactc ctgaagaacc caaattgatc tgagcatcca ctgcttttgt caacaggttg
     3301 aatctcacta tcctgggcgc cccgctgaga cctatataca tatacgttcc atcctctgat
     3361 acggccatta ctccgggttc gcttcctata aaataacaag tatctatcgt tccgaaatat
     3421 ggatcaataa cacataagct atttcctgtc gcttgtccgc tcggcgtcgt aacgaacaac
     3481 ctgtcttgtt gaggcacata aatcattgcg ttattatcta tgggaacgcg tttcatatca
     3541 ttttgcccaa agactccatt cggtacaaat aacaagctcc acgcaagtag cgcaatatac
     3601 tgcatgacat cagaattgtt acgagtttgt cttcttctct tcatggcatt taggtttaca
     3661 ggatttgagt tgcctcaata aaacaacaga cgttgagtct gcacttactt cactgcaaag
     3721 gtaaaaaaat caaaaatata aagagaaaaa actatgagca ctttgcgact ttaatgactt
     3781 cccatccgtc cacttcctgc acgccgatgg cggtttactc tgccggcgcg aggttcatac
     3841 ctggtgcccg ctatcaggcg ggttttatcc gcaaaaaaga cctcactcca actttccctt
     3901 ctccttattc catttcagca caatttcctt gcgctgcacc tgccggaaca gcccgcagta
     3961 gtcggcgtat tcgttatcgt cgccgcggca ataaatctgc tccaaaccgg tataaaccac
     4021 cgccttcacc gaggtgccct gtcgcgtcag ttcgagcgaa accggcagca gttttttcac
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtcg
     4261 gccatgcgaa acaaggccat gcggtaggtc cactcgccgc cgccggtgcc ctcgtccacg
     4321 atctcgatga agccgttttt gaggtccacc accgcggcaa tgggttcttc tgtgtccggc
     4381 gacatgctcg tccattggcc cttgttttct ttcagttcgt agcccgaccg gtacggctct
     4441 ttcaggcggc ggtagtagta gagcacgtcg cggcgctcct gcggcacgtc ttcctccacc
     4501 gttggcgcgg cgaggggagc ttcctccgtt gctgccggag cggcagaggt ggtggccggg
     4561 gccttgttgc agccgaggca ggcgaagatg gataagagca gaagtgattt tttcatcatt
     4621 ggtgtattta ttcccccatc aattacaccc caactgcgcc ctcgccagcg gatcattgaa
     4681 cgggaaacac tgccccgaca aaaccctgat gggctggtag cgcctgagat tgggatcgtg
     4741 gagcgacttg gtgaggaagg ccgtgatgtc ggcgatttcc tgctcgctca agctcagtgg
     4801 cacaaaatac ggatcgagct gagaggccgg tacgttggga ttttccggca aggcccggtt
     4861 tttgtactcc accacctgcc ggatgctgcg catggaagca ccgtgaccaa agaaaggcga
     4921 atcgctcatg ttgtagagct gcggcgtttt gaacttgtgc atgtcttcgg ctcgaccagt
     4981 gaagccgccg cgccccaggt gttcgggagc attgcccggc gttttgaata ccggttcggg
     5041 gcattcattc aggcccttca tgcccagcgc gtggaagttc atgttgttca atgcggggcc
     5101 ggtgtggcag ttggcgcatt gggccttgcc gaaaaacaga atggcgccgg ctttttcggt
     5161 ttcgctcatg gcattggatt gaccgcgcaa ccaaagctgc caaggcgact gattggccaa
     5221 aatggtgcgt tcgtaagcgg caatggccaa gccggaggtg atgttggtgt nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ttgggcgtgc cggccgtcca
     5461 ggcgtgttcg gtgccgatgt tcatgcccac gccgccgaac tgtccgttcc acagcatcag
     5521 ctcctgataa gccgtgttga gcgctgccgg agagcggatg ggctgcacgt ccatgttgtt
     5581 cacgatgtag ttgatgtctc gctcgcggcc ctcgccgttg aggccaaaac cgatgccgcc
     5641 ctcgccgatg ccctgaaagc gcccggcctg aaatccggcg gaagcgaaat ggcaggaagc
     5701 acaggaaaag cggccctgtc cgatggcttg ggcaggttcg aggccgaatc cggtttcatg
     5761 gaagagcagg cggcccagtt ccactttggc tttggtgatg ggatttttgc gggatggcgg
     5821 cgtaatcatc ggaggcgggc atgcggaaga aagcgcggcc tgcgccgttg gaggcggttt
     5881 gcagtacttc atccaattcc tgatccaatg cagaggtgtc gtcgtccgga cggcaggcgc
     5941 ccatgagcaa aacgagtgct gcaacgagca caaagtagtg acgtttcatg ttggaaagat
     6001 ttataagtaa tggaaaatgg tagcggcaga gcgcctctct tgctcgtttc gatgcaaagg
     6061 taaggcaatt ttctcatctt tgtcaattat gaaaaagtta aatacatgga acgacttttc
     6121 ggactgatcg gctacccgct atcacattcg ttttcaaagc gttattttac agaaaagttt
     6181 gaacgcgaag gcattgccgg ctgccgttac gagctatttc cgctggagcg gatcgaggat
     6241 ttgctcacca ttctggagga aaatccctcg ctgtgcggcc tcaacgtcac catcccttac
     6301 aaacaggcgg tcattcctta tttgcaccag ttagatgccg gcgcgcaggc catcggagcg
     6361 gtgaacgcca tccgcattgc cgacggaaaa ctgaccggct acaacaccga cgcgtatgga
     6421 tttatggagt ccttgcagcg gtttttacaa caatcgggcc atcagcctcc gggcgcgctc
     6481 gtgttcggaa ccggcggcgc ggccaaagcc gtcgtgtggg ctttgcagca attgggtatc
     6541 ccgcatcttt cggtttcccg gcgggcagaa gcaggcagca ttctttacga aagtattgat
     6601 aaagaacatt ttacaacgca tccgctcctc atcaacacca cgccgctggg catgtcgccg
     6661 cacaccgacg cctgccctcc cctaccctac gacttgctgg atcggcagaa tcttttgtat
     6721 gatttggtat ataacccgga ggaaacgctt ttcttgcaaa aaggcaaaga agccggctgc
     6781 catactacca atggcttgga gatgttatat ttgcaggccg aaaaagcctg gtcggtttgg
     6841 aacgacccgg cgctctgacc aaatagctta ttcaaaccaa cactatggaa tcatccggca
     6901 agcccgtgcc caaaaacgta gaaaaaatgc tcgaagcctg gcagcaatcc ggcgacgagg
     6961 cctccctgcc gctccccaat tttgcagata ccgctaccgc gttcgccgtc aaaaccgatc
     7021 aggaattgga aaaagccgcg cgcctcttcg gactgatgaa tcaccattgg ttggtgggca
     7081 tgggttcgat gttgggcctg gcagccatcc gactgagaat gccgtttgtc gaaagcgtgg
     7141 taaaaaatac catttttgag cagttttgtg ccggcactac cctcttggac agtgcgccga
     7201 acatccaaaa actgcacggc gccggggtgt attccgccct cgattacggg gtggaagcca
     7261 aagaaacgga ggaggatttt aatcatacga tgaacgagat catccgcgcc attgagttct
     7321 ccgagcagtg tccgggcatt cggctcatca gtgtaaaaat aacggggctg gcgcgtttcg
     7381 ggctgctgga ggctgttcac aacaaaggca atctcaatcc ccgcgaacgc aacgagtaca
     7441 aaaacgtgct gaagcgcata gatgccatct gccataccgc cgatcagaag ggcatcagcg
     7501 tttttataga cgccgaagaa tcctggattc aggatgccat agaccatttg gccaccctga
     7561 tgatggcgcg ctacaaccac gaacgggcag tggtgtataa tactttccaa atgtaccgca
     7621 ccgaccgcct gcaatttctc atccggtctt acaatcgcgc cgctgccgac ggatatttcc
     7681 tcggcgcaaa attggtgcgg ggcgcgtaca tggaaaaaga acgcgaccgc gctgaacgcc
     7741 tcggctatga atcgcccatt catcccaaca aagaagctac cgaccatgct ttcaatacgg
     7801 gggtgcgctt ctgcatggat cactacgaga aaattgcgtt ttgcaatgct tcccacaacg
     7861 ccgacagcgc catgctgcag gccgaactca tccgcgaacg ccaattgccg cgccaacatc
     7921 cgcacctgct cttctgccaa ctctacggca tgagcgacaa cctgactttc aacttggcca
     7981 aagcgggttt caatgccggc aaatatgtgg tatatggccc cgtgcgcgag gtggtgcctt
     8041 tcctcattcg ccgcgcacag gaaaacacaa ccatcaccgg cgatatgagc cgcgaacatc
     8101 aattggtaac gaaggaactg aaacgcagag gcgggtaggg ttcaaggttt cgggttggaa
     8161 cggctgctgg cctcaagctg ctggctgctg gctacaagct gctggctgtg aacactctac
     8221 gcttcgcacc ggctaccgcc caccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ccttgctgag ctgcggcagc gtcggctgaa
     8341 tgtgattcca aatccaaacg gcgaggtttt cggcagtggg attctccagt cccggaatct
     8401 cgttgagcaa cttgtggtcc aactgttttt ccaaaggccg ccacgcatct ttgatgtcgg
     8461 caaaatccgt cacccagccc aagaccggat gcaattcgcc gcgcaaatac agcttcacaa
     8521 tataagtatg cccgtgcaaa tgacggcatt tgtggtcttc cggtacatac ggcagccaat
     8581 gcgccgagtc aaaagtgaat tctttgaata tttccatcat ggtataaaat gcaatgcttt
     8641 tttgttcaaa gttcaagatt tcgggtttca tccgccgaag ccttggcaaa ggcgggttca
     8701 aggtttcgag ttcaagattt cggattcctg attcctgatt tcggattcca cccgacgata
     8761 aactgtgaaa cagattacca accagatgct gccccgacga tgaactgcgc agcagattac
     8821 caacccgttc ctaccccgac gataaactgc aaagcannnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnca ctctacgctt cgcaccccgc gccacaggcg cggctaccgc
     8941 ctaccgatcc actgcctacc gatccaccga tctacgcctt caacaccgcc cctgccagct
     9001 tcctagcctc ctccttcgca tcccgccacc aggccgccga ccacaccgaa atatactcga
     9061 tgttcttgtc tttcaaatgc ttgcgaattt cgtattccca cagccagtcg gtttccggcg
     9121 tgtcgtaaaa actgccgtcg gcccgcaacg cagccaccgg acgccgcata tcggcatcgn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgcc gggcgacatg cggtctttgc
     9241 cgatgtacga ggtcagcacc gccgccactt cctgatggaa taccgagacc tcctgtgcgt
     9301 gctcgccttt gcgcaccagc gccgccaccc gctctatgat gctgcgctgc tctttggctt
     9361 tcgattgccg gaaggcagag aggaagagca ggtaattggc caacaggcac tcgccgcggc
     9421 gggaagtatc gccggccatg cgttgcaggt ctatgtcgtc tatcgagcac agggcgtgta
     9481 ctttcgtcga agcccggctc atcagcgccg ccaattgcgc ggtctggtcc ggtccgttca
     9541 gcatttctat gtggccgctc accttgccgt tgggcgccga ggggccgaag gtggctgaca
     9601 aaatcatctc atcgaagtgc aggccgggca actcgccgag gtggaaaacg cccagtccgt
     9661 tgcgctccaa ttgttgcacc acatcggcgc cgggttcgtt gcgctgcttg attctcagca
     9721 ggtagtagct gatgaggtcg cgttgttgcg gggtggcgca cacgatcccc acgctgggca
     9781 tggtgcgctg cggcgtgggc tgtatgcgat tgagcaattg cagcgccgcc tcggcttctt
     9841 tttcgttgat gccgctgtgt tcgtaataaa aaccgtgcgt gtgcgtcagt tgcaactcaa
     9901 aaccgcgggg cgcctcgtcg gccacagcta ctttccaggc cgactggagg agattgccgg
     9961 gcttgagctg gtgtatgatg cccaagggca aaatccggta gcccgccgcg cggaacaagc
    10021 ccaacaacga agcgtcgtcg cgttcgggct gctgggccgg atcgccgacg gccaatgcgc
    10081 gccgccccaa atgcaacaag ggcgcggcct ccgctgccgg tacggcctgc gcttcgtcca
    10141 cgatcaccac atcgaacccc gtatcggcgt tgaacaaagc ctgcgccacc gccggcggag
    10201 ccaatacgat ggggaatata tcggtgatgt gccgcccgct ttcgcggaac aggcccggca
    10261 agtccacgcc tgcatcagcc gtgccgctca gggcctgcga ggttttgcgc ccgccgcgtc
    10321 gcagttcttt ggcgacagcc tcccggcgtt cgtcccacag cgtgcgcaga cgcgacagaa
    10381 gcaggggttg caggcccgcc atcgtttcgg ctaattcttc cactgcctca tccgacaccg
    10441 gcagatcggg cgaaaattgc atcgtcaggc agttgttgaa gtaccaactg tctaaggcgg
    10501 ccgtccagtc gttgggtttg gctttggcca atgcgcgcac tattttccgg ccgttttcgg
    10561 gcataccgat ccagtgtcgc tgccattcgt aaaaagtgtc gtagtctttg aggtacaggc
    10621 cgatgttgtc gaaactttcc atcacctctt ccaggtactt gaggcgtttg gtgagcgtga
    10681 gcgcctgatg ttcaagtggt tgttccaaaa tccccgccag attgacgtcg ttgagcaatg
    10741 aatccagacc ctgttccaac tccgtcacga tggtggtgta aggcagatct gcatgtacgc
    10801 ttttgctgct gaggcgctgc acttcttcct gaatctgagc gggcaattgt tcgttccaga
    10861 ggccgaacga agcctccatt tccaagacat tggctctgat tttggccatt gacttgcggt
    10921 ccttttcggg caatacggca aagtcgaaca gaggccgggc cgtccagatg cgctccaatt
    10981 cggcgaaagc ctcccgcacc tgctcgcggg ccgtcagaat ctgctgtgcg cgcgccgaca
    11041 cgatgcgaga cagtttgagg ccgccttcgg agctgttttc aaagtcggcg ccccattggt
    11101 tgccatagtc cgtcaacata cctttgaggc gaaaaatggc ctcattgagt tgttggcggc
    11161 tgccctgata gaggtcgttg agtttgtcgg aatagccgtt gagcgtgctg atgtagcggt
    11221 ggtgaaggcg agcgctgcgt tcggaaaacg cggctatttg ctgccggatg taggccagtc
    11281 cgccgtcttt gtctttttgt gtaaacaact cgcctttgag cgccgacagc gggtgtttga
    11341 gcgtattgat acttggaaac agcacctggc tttgcgcaat gccccggcgc agcacctcgt
    11401 attcttcaaa agtaaaagca aagtcctgat tgtaaagctg ttgccccagc agttccttgc
    11461 cggctctgct ttggctgcgc aaaaacaggc ccacggtttc ggtccagttg tgcgtgccga
    11521 aaaccgggcg gcgcgtggcg tggtagccgg catcgagttt gtctttgagg cgcaaagctt
    11581 tctctaagag gagtttgtag cggtcggccg ggaaagcctc cacgatgggc ggggctgaga
    11641 cggcgctgcg taacagaccc agtaccgccg cctgatcgga ctgcacattg cggatgnnnn
    11701 nnnnnnnnnn ntccgcagcg gcccagcacg gcctgcgctt ctttgagttc cgacaggcgc
    11761 gagcacacca tgaggcattt gcggccattg gcgagcgcgt tgatgatgac gttggcaatg
    11821 aggttggttt tgccggtgcc cgaaatgccg gcggccaccg tgatgcgcga gcgctctacc
    11881 tgctgcatgg ccgtagcctg ccagggatcg agcgggagca ggctgaacgg atgtccctcc
    11941 acgggaagcc tgttgtgcgg gcagcaccgc ttcgccggag ttgtacgccg cttcgggcag
    12001 caaacccagc aaaccggacc aaatcaggct gccgttgtcg aggcaggcgg cggcttccaa
    12061 ttccgcatcg ccggggcagg gcatgatggt gagattgtca acgggaacgt gcaggccggt
    12121 ttcgcgggcc agttccaagg ccagcaagac catttcgctg ccgctgggtt tgcgctgcgc
    12181 cgtgatgcct gtgtaacgag cgctcaacgc cgggctgcat tggcgttcca gccaggccgc
    12241 cagcaccggg ttgagcgggg acagcaccga ccaaggccaa aaaaccccca tgagtccgta
    12301 ggccagcggg gcggccggca gtaatccacg caccgtgctt cgcaaatcgc gcgccgccgc
    12361 tcgccagcct tgccgtcttg cggtcgccat caagtagacc gcgacagctc cgaaaaaata
    12421 gaagatcacg aagaaggcgc caaccc
//
LOCUS       DAORLF010000084        12351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2736_length_12351_cov_2.093445, whole genome shotgun sequence.
ACCESSION   DAORLF010000084 DAORLF010000000
VERSION     DAORLF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_12351_cov_2.093445"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK198_08875"
     CDS             complement(<1..256)
                     /locus_tag="PK198_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HRF38890.1"
                     /translation="MHSTNYFNTFIEIAEDCPVAAAEIPPLKGDNKSVANLQFEMILE
                     HPYGYTSDDVVFGVYALKNGIDSGLEDARAAFFSKGQACLR"
     gene            complement(249..674)
                     /locus_tag="PK198_08880"
     CDS             complement(249..674)
                     /locus_tag="PK198_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF38891.1"
                     /translation="MAFSLKTQLTINAPVERLWAALTQPELVKQYFFGTDLVTTWEPQ
                     TPIYFRGEWEGKPYEDKGRVLSFVPLQSLAYNYFSSWSDVEDKPENYQIIRYRVKPLG
                     AKTVLFIEQHNIDTIDKKVHSLQNWKGLMVEIKKLLEHA"
     gene            complement(731..1456)
                     /locus_tag="PK198_08885"
     CDS             complement(731..1456)
                     /locus_tag="PK198_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRF38892.1"
                     /translation="MNRIDRLMGIVTHLQAKKYLSLEQISTHFGVSGRTVFRDLKALS
                     EIGIPVCFEPEKGYTLVQGFFLPPVSLTVEEANALTLAEPLVLRFADKSVQQHYGTAL
                     AKIKMVLGRMQREKMETVQAGTAHFVPDHYAHLMPATEYLIPLQNAVSNKNIVRIEYL
                     NQRDELSRREVEPIGLTFYSLNWHLIAWCHSKNDYRDFRISRIRQLSPTMQPFRKTNH
                     LALHEYLQKMQQEIFQNSQHPLT"
     assembly_gap    1733..1766
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1808..3229)
                     /locus_tag="PK198_08890"
     CDS             complement(1808..3229)
                     /locus_tag="PK198_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38893.1"
                     /translation="MKNARIIQSLLLVLFCWTANTAVIHAQAPAGSGTEAGKAIEEIF
                     EKAKPDKSRKDDVSVKKRAALRELERAVKKAAKDNPNNADAVKDVVKAKLEELSGEKK
                     NKVIEDFLKDLKDKILKSKKGKKFTFRNDTGRNTNDLHLVLSGETRPINVSEDPTTGV
                     KKVGGVYENYPGEKSKKHDYKTGGIVTPGSSVEIQMEEETEVISWYWTFDGEKIGDGE
                     YGYEGTTVISVVPLPDPFRPDRATVSVGIGASLPFQNPEGIYISPVQLHDNFTRSIAT
                     QPTVIEQIMERMEGVFVPGVNTHPSQHTLPDATLQASTQIMPGLQVGFSLSRNLEFSL
                     GAHYFSSTFTGRLPITVFPFENPALRIEYGAINASSNGLLIDISLRYLLPSKKVRPYL
                     EAGARRLSVLQNETHLEIAGLQLPFDEIKTASGFSAYAGAGVRAYFGANVYLQAGAML
                     SKWPGSGYAPGGHVSVGWTFGRR"
     gene            complement(3443..4519)
                     /locus_tag="PK198_08895"
     CDS             complement(3443..4519)
                     /locus_tag="PK198_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38894.1"
                     /translation="MKYIFPFLLLPLFLCAQTPPGCGSSDLRYLSWDTELSSARLHFA
                     ISPPLPGVQTYFLNYTLNAHSNPAPGMLEVSFPGVPQSFGPAPLGGLHYFTAQNHCAD
                     GSIHTGATIGIDMRNADPECPAIRDFMLLDLQPVFIAFAWGAAPQADSFRVRYQADAL
                     PIQVGFAPEPVWEQPLAAATVHTFRIAALCTVPGLVQQAVVQGPEFIFSIIIIDDIKA
                     IAVPVDTAAIHTALCNGYRIQCDHGDYGTDKERFISDHPQLGIPYPCPAVVAAQEIAP
                     EIAGLRLSPNPTGENLQADFLLRHSGEVQWEVFDPTGRILHRQKQWLAAHRIRYEAQA
                     LPAGMYLWRLQSGGQFFSARWVKY"
     gene            4578..6050
                     /locus_tag="PK198_08900"
     CDS             4578..6050
                     /locus_tag="PK198_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38895.1"
                     /translation="MHVRLLELMRRLTAGELAELSLFIQSPFFNRSEKTAALFQYLML
                     YAPHFEAAQLRAEGAFAAVWGAGKPFDKAALNRLGSQLFKLTEQFLAHAALSEDSQRQ
                     ALYLMQQYDQLELESCLSAVLQRLQSPELQGEASLNRLALQLETELMLADRQHRNDDR
                     TGEANLQALNDSLDLYFVTFKLKLLNAMLSRGAVSKTDFRMAWKESVVEALQQSPEWR
                     TNPLTELQYLMWQVLEQPGDVPAYNALHEFLLGQRQALPASDLRDGFACLQNAAKRVW
                     PVQEYYLRLFDLYRDQYEGGILHENGKLHHAIFRNIVNSALLTGQTDWAAQFIQNQRH
                     CISPEAYRDAAYYQNSALLYFHQKQFDKALDSLRQSKPADVYYKLADRSLLARLYYEQ
                     RESEALDNLLNTFSKFVFDQQKKIAAPKVKSYRLFINSLRQWHQLLHAQPALYRKWLR
                     HQAASDANEKTAVQAFLDETAAAPVFYGQKWLMEKAEEML"
     gene            complement(6053..6598)
                     /locus_tag="PK198_08905"
     CDS             complement(6053..6598)
                     /locus_tag="PK198_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF38896.1"
                     /translation="MYSELRFLAAMLPVILFSLAVAPLSGQSDSPASVIIGPVFKGCE
                     NAPAEQVDICCQRKMLESIYRNIRYPRQAREEGDQGTLVVQFTITKTGETANIEVIKS
                     ISHSCKDEVIRVVKELPRWIPATVDGEPVDAVVSLPVEYRLDGVPLKKRQQIAEASFD
                     WNSLQGIVLQRVIVTAYAATR"
     gene            complement(6676..6969)
                     /gene="raiA"
                     /locus_tag="PK198_08910"
     CDS             complement(6676..6969)
                     /gene="raiA"
                     /locus_tag="PK198_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRF38897.1"
                     /translation="MKVHTETVAFKADQKLIEYIERKLSKMDQFFDRIIDAQVVLKLE
                     NAGQVRDKIAEVRLNVPGEILVAKESRKTFEASVSSAVEALRRQLIRYKEKAA"
     gene            complement(6993..7898)
                     /locus_tag="PK198_08915"
     CDS             complement(6993..7898)
                     /locus_tag="PK198_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF38898.1"
                     /translation="MILNAFMQYLEFEKRFSPHTLLAYESDLRQLERFLAATFEISDP
                     KDITPMHLRAWMVDLLGNEASPRSVRRKISSVKSWFRFLRKRTEVSHNPAQNLILPKT
                     GKRLPVVVEQKDMQQLFEQVAFGTGFAAERDRTLLELLYQAGLRRSELMLLSVSDIDF
                     SRRALRVSGKGNKERLVPFGTALEERLRQYVRARQAEFPATAETALLLGDGGKRMYPK
                     QIYNIVNQYLSAVTTVEQRSPHVLRHSFATHLSDNGADLNAVKELLGHSSLAATQIYM
                     HNSIERLKQVYLQAHPKAGRRPDNK"
     gene            complement(7968..>9798)
                     /locus_tag="PK198_08920"
     CDS             complement(7968..>9798)
                     /locus_tag="PK198_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRF38899.1"
                     /translation="HHFAMMAGLHWHWLENAWVQLALCLPVYVLGAWHFGRSALGSLR
                     SGVPNMDVLIFIGSTAAFIYSLIGAVLGEPKYIFFETAATIITLVLAGNWLEKRAVAQ
                     TTTIGELSRLQVEKARKIMPSGVVVLIDKDDIAKGELLQVNEGDKIPADGIVEAGNAW
                     VDESMLTGESLPVEKRPGDTVIGASLLQSGNMRMRVQAAGRDTVLSQMIELVRTAQQD
                     KPAIQRLADRISAVFVPVVVSIAALTFLIGYFGFQISPQQALMNAIAVLVISCPCAMG
                     LATPTAVMVGVGRLARNGILVKGGQTVETLAQIRNIVFDKTGTLTTGAFRIKNIALHT
                     PDAHKIYALIHHLEQHSSHPVAQALVQEMNGCMNGTVVELQDIEEQKGVGVSARDAQG
                     NVYKIGSARILPDGVASGPAAVFLTQNDQLLAAIELDDEMKPDAAETVSFLKKQHIHP
                     VILSGDKAEKTGAVAASLGIQDYYAEQLPAQKLERIAALSAQAPTAMVGDGINDAPAL
                     SRATLGISLSNASQAAIQSAQIILLNGRLDRLRDALHISKHTLLTIRQNLFWAFAYNI
                     VAIPLAAAGYLSPMWGALFMAFSDVVVIGNSIRLKTKKLNLPS"
     assembly_gap    9799..9930
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10322..10355
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10356..>10856
                     /locus_tag="PK198_08925"
     CDS             <10356..>10856
                     /locus_tag="PK198_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRF38900.1"
                     /translation="VIGYLSIVFEHPLKLDKSVPALLMGSLIWAVISIGGVEIVGHDH
                     HTTTMENGLLHHLGKTAEILVFLLGAMAIVELVDLHKGFSVITDFIKTRNKRRLLLVL
                     SILAFFLSAVLDNLTCTIVFISLLRKIIPEREDRLWFIPFIVIAANAGGAWSPIGDVT
                     TTMLWI"
     assembly_gap    10857..10866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10924..>11253
                     /locus_tag="PK198_08930"
     CDS             10924..>11253
                     /locus_tag="PK198_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRF38901.1"
                     /translation="MAVPVLIGFLSKRFAGELPPPTEKQLALAGQQKKLLSSKAMLIA
                     GIGGLLFVPIFKTVTHLPPYMGMMLSLGIVWLISEYIHPEENFDEERKEMYSARKALS
                     RIEMTSII"
     assembly_gap    11254..11337
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11338..11678
                     /locus_tag="PK198_08935"
     CDS             <11338..11678
                     /locus_tag="PK198_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRF38902.1"
                     /translation="AEKLRHVIPNQDIVVVILGFLSAVIDNVPLVAASMGMYPMDLYP
                     MDDHIWQFIAYCAGTGGSMLIIGSAAGVAAMGMERIDFIWYLKHFAWIAMLGFLAGAA
                     AYLLMLPIFE"
     gene            11692..12078
                     /locus_tag="PK198_08940"
     CDS             11692..12078
                     /locus_tag="PK198_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38903.1"
                     /translation="MFPSIKKILPAALLLLAVGCRQDPHRLMAGHWQVVDISSKEELP
                     EYRLDDFRFFFTEDGRYQYFGNLYYEEAGVYTVEGSYLHTTDTLRQDTGEKTVQILKL
                     TKDSLFLRMADETTMKLAKDKIETPK"
BASE COUNT         2714 a         3125 c         3250 g         2968 t
ORIGIN      
        1 cccgcaggca agcctggcct ttggaaaaaa acgctgctct ggcatcttcc agtccgctgt
       61 caataccgtt tttgagtgcg taaacgccaa aaaccacatc atcggaagtg tagccgtacg
      121 ggtgctccag aatcatttca aactggagat tggccaccga tttattatcg cctttgaggg
      181 gcggaatctc ggcggcggct accgggcagt cttctgctat ttctataaag gtgttgaaat
      241 agttggtact atgcatgttc cagcaatttt ttgatttcca ccatcaggcc cttccagttt
      301 tggagcgagt gtactttttt gtcaatggtg tctatgttgt gttgttcaat aaacaggacg
      361 gttttcgctc caagcggctt cacccggtag cgaatgatct ggtagttttc gggcttatcc
      421 tccacgtcgg accaggaact gaagtagttg tatgccaatg attggagcgg tacgaaagac
      481 aacacccttc ctttgtcttc ataaggtttt ccttcccact cgccccggaa ataaatgggc
      541 gtttgaggtt cccaggtcgt taccagatcg gtgccgaaga aatactgttt gaccaattcg
      601 ggttgggtga gcgctgccca gaggcgctcg acaggtgcgt tgatggtcaa ttgagttttt
      661 aatgaaaatg ccatttgatt gggttgtatg atgaaagttg ccacaaagaa acggcccgcc
      721 actgacaacc ctatgtcagc gggtgttgag aattttgaaa aatttcctgt tgcatttttt
      781 ggaggtactc gtgcagggcg aggtggtttg tttttctgaa aggctgcatg gtgggcgaga
      841 gctgccggat gcgggagatg cgaaaatcgc ggtagtcgtt tttgctgtgg caccaggcga
      901 tgagatgcca gttgagggag taaaaagtaa gcccgatcgg ttctacctcg cggcggctga
      961 gttcgtcgcg ctgattcagg tattcgatgc ggacgatgtt tttgttgctg acggcatttt
     1021 gcaaagggat cagatattcc gtagcgggca tgaggtgggc gtaatgatcg gggacaaaat
     1081 gcgccgtgcc ggcctgtacc gtctccattt tttcccgttg catccggccc agcaccattt
     1141 tgatctttgc caatgccgtg ccgtaatgtt gttgcaccga tttgtcggca aaacgcagca
     1201 ccaatggctc ggcgagcgtg agggcattgg cttcctctac ggtgagcgac acgggcggca
     1261 gaaaaaaacc ttgtaccaat gtgtagcctt tttccggctc gaaacaaacc gggatgccga
     1321 tttcgctcag cgccttcaaa tcccggaaaa cggtgcgccc actaacgccg aagtgcgtcg
     1381 aaatttgttc cagcgacagg tatttcttcg cttgcaggtg ggtcacaatc cccatgaggc
     1441 ggtctatacg attcataatg tattgattgt aaggtacatt tatgaaatgg acatttctga
     1501 gagcagtgtg taattaagta agtttgcttc agcagtttag tgaaggggtg actcagccaa
     1561 gacacagccc ggtagtcacc ccttcactac gcattgcaat ttaatttaca cactacgaag
     1621 ggcactatgc ctctccgcta cttacatggt gcaagttttg aaaaaaatcc catccgtttt
     1681 gaattttcaa cacaaagaaa aaaacctgcc gaggtgccgc cccgacaggt tgnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnagaa tttttcaacg ggcaaaattc aggccgcatc
     1801 atattttcta cctcctcccg aaagtccagc ccacgctcac atggccaccc ggagcatacc
     1861 cgctgcccgg ccacttgctc agcatggcgc cggcctgcag atagacgttg gccccaaaat
     1921 aagcacgcac acccgctccg gcataagcgg aaaagccgga agccgttttt atctcatcaa
     1981 aaggcagttg caagccggct atttccagat gcgtctcgtt ttgcaacacg ctcagccgcc
     2041 gagcgccggc ctccaaataa ggccgcacct ttttgctcgg cagcaagtat ctcaaactga
     2101 tatctatcag cagcccattt gaagaagcgt tgatggcgcc atattcgatg cgcagcgcag
     2161 gattttcaaa cgggaacacc gtaatgggca aacggccggt aaaggtgctg ctgaaatagt
     2221 gcgcgccgag gctgaactcc agattgcgcg aaagcgaaaa ccccacttga aggccgggca
     2281 tgatctgagt ggaggcctgg agggtagcgt cgggcagggt gtgttggctg ggatgggtgt
     2341 tgacgcccgg cacgaacacg ccttccattc tttccataat ttgctcaatt actgtgggtt
     2401 gtgtggcgat gctgcgcgtg aaattgtcgt gcagttgaac aggggagatg taaatgcctt
     2461 ccgggttttg gaacggcagg gaagctccta ttcccacaga tacggtagcg cggtcggggc
     2521 ggaacgggtc gggcaagggt acaacggaga tgacggtagt gccttcgtag ccatattctc
     2581 catcgccgat tttttcgccg tcgaaggtcc agtaccagga aatcacctct gtttcctctt
     2641 ccatttggat ttccacagag ctaccgggcg tcacaatacc gccggttttg taatcgtgct
     2701 ttttgctctt ttcgccgggg taattttcgt acacgccgcc cacttttttg acgcccgttg
     2761 tcgggtcttc gctcacgttg atggggcggg tttctcccga caagacgagg tggaggtcgt
     2821 tggtattgcg gccggtatcg ttgcggaagg tgaatttttt gcctttttta ctttttaaaa
     2881 tcttgtcttt caaatctttg agaaaatcct caatcacctt gttctttttc tcaccagaca
     2941 attcttccaa ttttgcttta acgacgtctt tcacggcatc ggcattattc gggttatctt
     3001 ttgctgcttt tttcacggcg cgctcaagct cccggagtgc cgctcttttc ttgacggaaa
     3061 catcatcttt tcggcttttg tcgggttttg ccttttcaaa tatttcctct atggctttgc
     3121 ctgcttctgt gcctgatcct gccggcgcct gagcatggat tacggcggtg tttgccgtcc
     3181 agcagaagag gacgagcagt aaactttgaa tgattcttgc atttttcatg gctgatgaat
     3241 tttgagttaa aaaatgttga caccgcaaaa atgcccccac tcgctaccca aaagccatta
     3301 cattttcagt cgtttacgcc ggtacaaaat ccgtcgcaaa acacagtcct ttgctgcgta
     3361 tcaatgatgc aaacatttaa taatcaacat tttatttaca aatttttcat tacctgaaaa
     3421 ggcgataatc ttttcaaacg cctcaatatt tcacccaacg agcagaaaaa aactgcccgc
     3481 cggactgcaa tcgccacaga tacatacccg ccggcagggc ctgcgcctcg taccggatgc
     3541 gatgcgctgc cagccattgc ttttgacggt ggaggatgcg cccggtcggg tcaaaaacct
     3601 cccactgcac ttcgccggaa tgacggagta aaaaatcggc ctgaaggttt tcgcccgttg
     3661 ggttgggact gagcctcaga cccgctattt cgggcgcaat ttcctgcgct gccaccaccg
     3721 ccggacaggg atatgggatg cccaactgag gatggtctga aataaatcgc tctttatcag
     3781 tgccgtagtc gccgtgatcg cattgtatcc ggtagccgtt gcacagcgct gtatggatgg
     3841 ccgcagtgtc caccggcacg gcgatggctt tgatgtcgtc tatgatgatg atggagaata
     3901 tgaactccgg cccctgcact actgcctgct gcacgaggcc gggcacggtg cagagcgccg
     3961 ctatgcgaaa agtgtgtacc gtagctgctg ccaatggctg ctcccaaacc ggctccggcg
     4021 cgaaccccac ctgtatgggc agggcgtcgg cctgatacct caccctgaaa ctgtcggctt
     4081 gtggcgcagc gccccaggca aaggcgatga agacgggctg gaggtcaagc agcataaaat
     4141 cccggatggc cgggcattcc gggtcggcgt tcctcatgtc tatgccgatg gtggcgccgg
     4201 tgtgaatgga gccgtcggcg cagtgatttt gggcggtaaa ataatgcagc ccgccgaggg
     4261 gcgcaggtcc gaatgactgc ggaacgccgg gaaaagacac ctccaacatg cccggcgccg
     4321 gattggaatg cgcgttgagc gtgtaattca ggaaatacgt ctgcacgccg ggcaatggcg
     4381 gagatatggc aaaatgaagc ctggcgctgc tcaattcggt atcccaactc aggtagcgca
     4441 ggtcggaact gccgcatccg ggaggcgtct gggcgcaaag gaagagggga aggaggagga
     4501 agggaaaaat gtatttcatg gcgaatcgga tgtttggttg agcaatttag tttatttttg
     4561 gctgatagtc aagcattatg catgtacgcc tgttggaatt gatgcgacgg ttgaccgccg
     4621 gagaactggc cgaactgtct ctttttatcc aatcgccttt tttcaatcgc agtgaaaaga
     4681 cggcggcgct tttccaatat ctcatgctgt atgctcccca ttttgaggcc gcacaactgc
     4741 gcgccgaggg ggcttttgct gcggtttggg gcgccggtaa gccctttgac aaagccgcgc
     4801 tgaatcggtt gggatcgcaa cttttcaaat taacggagca attcctggcc catgcagcgc
     4861 tgtcggagga ttctcaaaga caggctttgt acctgatgca acagtacgat cagttggaat
     4921 tggaaagctg cctctcggct gtgctgcaac gcctccaatc gccggaacta caaggcgagg
     4981 caagcctgaa ccgcttggct ttgcaattgg aaaccgaact gatgctggcc gaccggcagc
     5041 accgcaacga cgaccgcacc ggcgaggcca atttgcaggc gctcaatgac agtttggacc
     5101 tttatttcgt cacattcaaa ctgaaattgc tcaacgccat gctcagcagg ggcgccgtca
     5161 gcaaaaccga ctttaggatg gcttggaaag agtccgttgt ggaggcgctg caacaatcgc
     5221 cggagtggcg cacaaaccct ttgacggaac tgcaatacct gatgtggcag gtattggagc
     5281 agcccggcga tgtgcctgca tacaatgctt tgcacgagtt tttactgggg caacgacaag
     5341 ccctgcctgc ctccgacctg cgcgatggct ttgcctgcct gcaaaatgcc gccaaacggg
     5401 tgtggccggt gcaggagtac tatcttcggc tgttcgattt gtacagagat cagtacgagg
     5461 gcggtatttt gcacgaaaac ggcaagctgc accacgccat cttccgaaac atcgtcaatt
     5521 cggcgctcct cacggggcag acggattggg cagcgcaatt cattcaaaat caacgtcatt
     5581 gcatttcgcc ggaagcgtac agagatgccg cgtattacca aaattcagcg ttgctgtatt
     5641 ttcatcagaa acaattcgac aaggcattgg atagcctgcg ccaatcgaaa ccggcagatg
     5701 tgtattacaa gctcgccgac cgaagcctgc tggcccgcct ttattacgaa cagcgggaat
     5761 cggaggcatt ggacaacctg ctgaatacct tctccaaatt tgtctttgac caacaaaaga
     5821 aaattgccgc gcccaaggta aagtcttatc gcttatttat caattctttg cgacaatggc
     5881 atcaactctt gcacgctcag ccggccttgt accggaaatg gctgcgacac caggctgcaa
     5941 gcgatgcgaa tgagaaaaca gccgtccaag cctttctgga tgaaacggcg gcggcgccgg
     6001 tgttttatgg ccaaaaatgg ctgatggaaa aggccgagga gatgttatag cctcacctcg
     6061 tcgcggcgta agccgtaaca ataaccctct gtaaaacaat tccttgaagg ctgttccagt
     6121 caaatgaggc ctcagctatt tgctggcgtt ttttcaaggg tacgccgtcc aaacgatatt
     6181 caacaggcag agacaccacc gcgtccacgg gttctccgtc cacagtagcg ggtatccagc
     6241 gcggcaattc tttcactacc ctgatgactt cgtctttaca actgtggctg atgctcttta
     6301 ttacctcaat attggctgtc tctccggttt tggtgatggt aaactgaacg accagcgtac
     6361 cttgatcgcc ctcttcccgc gcctgccggg gatagcgaat gttgcgataa atggattcca
     6421 gcattttcct ttggcagcaa atgtccactt gttcagcggg cgcattttca cagcctttaa
     6481 aaacaggccc tataatcacc gatgccggtg aatcggattg ccctgaaaga ggagcaacag
     6541 ccaatgagaa aaggatgacc ggcagcatgg ccgcgagaaa acggagttcg gaatacatga
     6601 tgaatggagt tttgaaaagg ccggtcccgt ttacacaaga ccggcctttg ttgggtttaa
     6661 caataactat tctggtcagg cggctttttc tttgtagcgg atcaactggc ggcgcagcgc
     6721 ctccactgcg gaagacactg aagcctcgaa cgtcttcctc gattctttcg ctaccaatat
     6781 ttcacccggc acattgaggc gtacttcggc gattttatcg cgcacctggc cggcgttttc
     6841 caatttcaat actacctgtg cgtcaatgat gcggtcaaaa aactgatcca tcttcgacaa
     6901 cttccgctcg atgtattcga tcaacttctg gtcggccttg aaggccactg tttcggtgtg
     6961 cactttcatg ttccacatga atgaataaaa tattacttgt tatctggtcg gcgccccgct
     7021 ttcgggtgcg cctgcaaata aacctgcttg agccgctcga tggaattgtg catataaatc
     7081 tgggtggcgg ccaagctgct gtgccccagc aattccttca cggcgttgag gtcggcgccg
     7141 ttgtccgaca gatgggtggc aaacgaatgc ctcagtacgt gcgggctgcg ttgttccacc
     7201 gttgtgaccg ccgacaggta ctgattaaca atgttgtata tctgcttggg atacatgcgc
     7261 ttgccgccgt cgccgagcaa gagggcggtt tctgccgtgg ccggaaattc agcctgccgt
     7321 gcgcgtacat actgccggag ccgctcctct aaggccgtcc cgaaaggcac gaggcgctct
     7381 ttgttgccct tgcccgaaac gcgaagggcg cgccggctga agtcaatatc agaaacggag
     7441 agcagcatga gttcggaacg ccgcagtccg gcctgataga gcagttcgag gagcgtgcgg
     7501 tcgcgttcgg cggcaaaacc ggtaccaaaa gctacctgct cgaaaagctg ctgcatgtct
     7561 ttttgttcta ccaccacggg caggcgtttg ccggttttgg gcaggatgag attttgcgcc
     7621 ggattgtgcg agacctcggt gcgcttgcgc aaaaaccgga accaagactt gacagaggaa
     7681 atttttctgc gcacactgcg gggcgaagcc tcgttgccca gcaagtccac catccaggcg
     7741 cggaggtgca tcggcgtgat gtccttcggg tcggaaatct caaaagtagc ggctaaaaat
     7801 cgctccaact ggcgcagatc gctctcataa gccagaagcg tgtggggcga gaagcgcttc
     7861 tcaaattcca gatactgcat aaaggcgttc aaaatcatgc gcttcggtga ggtgtgtgcg
     7921 aaaaggttgc tttacgaggc tcaaggtaag accttctttg caaaaagtca agagggcagg
     7981 tttaattttt ttgttttgag ccggatggaa ttgccgatca ccaccacatc tgaaaaagcc
     8041 atgaacagcg ccccccacat cgggctgagg tagccggcgg cggccagcgg aatggcgacg
     8101 atgttgtagg caaaggccca aaacaggttt tgccggatgg tgagtaaggt gtgcttggaa
     8161 atatggagcg catcgcgcag acggtccaaa cggccattga gcaggataat ctgagccgac
     8221 tgtatggcgg cctgcgaggc gttgctcagc gagatgccca gcgtggcccg gctgagggcg
     8281 ggcgcgtcgt tgatgccgtc gccgaccata gcggtggggg cttgtgccga aagcgcggcg
     8341 atgcgttcga gtttctgggc gggcagttgt tctgcgtaat aatcctgaat gcccagcgat
     8401 gcggccaccg cgccggtttt ttctgccttg tcgccgctga gaataacggg gtggatgtgc
     8461 tgttttttca aaaaagacac cgtttcggcg gcatcgggct tcatctcgtc gtctaattcg
     8521 atggcggcca gcaattggtc gttttgagtg agaaacactg ctgccggacc agatgcaaca
     8581 ccatccggca aaatgcgcgc cgagcctatt ttatacacat tgccctgcgc atcgcgggca
     8641 gaaacgccga cgcctttttg ttcctcaata tcttgtagtt caacgacagt gccgttcatg
     8701 cacccgttca tttcctgcac cagggcctgc gccaccgggt gcgaggagtg ctgttcgaga
     8761 tggtggatga gggcgtatat tttgtgcgcg tcgggggtgt gcagcgctat gtttttgatg
     8821 cgaaacgctc cggtggtgag ggtgccggtt ttgtcgaaaa caatgttgcg aatctgggcc
     8881 agggtttcca cggtttggcc gccttttacc aaaatgccgt tgcgggccag ccttcccacg
     8941 cccaccatca cggcagtggg cgtggctaag cccatcgcgc agggacagga aatgaccaac
     9001 acggcaatgg cgttcatcag cgcctgctgg ggactgattt gaaacccaaa atacccgatg
     9061 agaaaagtaa gcgcggcaat gctcaccacc accggcacaa atacggcgct gatgcggtcg
     9121 gccaagcgct gtatggcggg cttgtcctgc tgggcggttc ttaccaattc aatcatttgc
     9181 gacagcacgg tatcgcggcc ggcggcctgc accctcatgc gcatgttgcc cgactgaagc
     9241 agcgatgcgc cgatgacggt atcgccgggg cgtttttcta cgggcaggct ctcgccggtg
     9301 agcattgatt cgtccaccca ggcattgccg gcttccacga tgccgtcggc gggtatttta
     9361 tcgccttcgt tcacttgcag gagctcgcct ttggcaatgt cgtctttatc aataaggact
     9421 accacgccgg aaggcatgat cttgcgggcc ttttccactt gcaggcggct cagttctccg
     9481 atggtggtgg tttgggccac ggcgcgtttt tcgagccagt tgccggccaa taccaaggtg
     9541 atgatggtgg cggcggtttc aaaaaagatg tatttgggtt cgcccaaaac agcgccgatg
     9601 agactgtata taaaggccgc cgtgctgccg atgaagatga gcacgtccat attgggcacg
     9661 ccgctgcgca aggagccgag cgcgctgcgt ccgaagtgcc aggcgcccag cacatatacc
     9721 ggcaaacaga gcgccaattg cacccaggcg ttttccagcc aatgccagtg caggccggcc
     9781 atcatggcga aatggtgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgaaagcg cacatctttg gtagaaaagc
     9961 tgacaaacac atcctgcatg cctttgcgct ccagaaaccg cgagataccg gcggcgcagt
    10021 tgttgcagtc cattccatct actttccatt ctatgatgcg ggcgtccatg atccttctgt
    10081 tttaaaaaat tgagaacaaa tataaaaggc aaaggattta ttgctcccct acccgatatg
    10141 atattttgat acaagctgaa tgtttttgca ccttgaatca ggggacgatt ctgcgtcttt
    10201 gacttcagcg aaccacacgc gccaatattt gtcatacact tattgctttc aagtatcctt
    10261 acactcgcag gaaagtttat ttttgccggg attttcatca aacaaaacgc aaccgacatg
    10321 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgtc attggctatc tttccatcgt
    10381 ttttgagcat ccgctgaagt tggacaagtc tgttccggcc ctgctcatgg gttcgctcat
    10441 ctgggccgtc atttccatcg gcggggtgga aatagtaggg cacgatcacc atacgacgac
    10501 tatggaaaac gggctgttgc accatttggg caaaaccgct gaaatcttgg tgttcctgct
    10561 gggggctatg gccattgtgg agttggtgga cctgcacaaa ggcttctccg tcatcaccga
    10621 ttttatcaaa acccgcaaca aacgcaggtt gctgctggta ctctccattc tggcgttttt
    10681 cctctccgcc gtgttagaca acctcacttg taccattgta tttatttcgc tgctgcgcaa
    10741 aatcatcccg gagcgcgaag accggctgtg gttcattcct ttcatcgtca ttgccgccaa
    10801 tgcaggcggg gcctggtcgc ccatcggcga cgtgactacc accatgctct ggattgnnnn
    10861 nnnnnnaaaa aagtgagtac cgagcagttg attctacgtc tatttattcc atccgtaata
    10921 tgtttggcgg ttccggttct gatcggtttt ttgtccaagc gttttgccgg cgaacttccg
    10981 cctcccaccg agaaacaatt ggcattggcc ggccaacaaa aaaaactgct cagtagcaaa
    11041 gccatgctca ttgccggtat cggcggcttg ctgtttgtgc ccattttcaa aaccgtcacg
    11101 cacctgccgc cctacatggg catgatgttg tcattgggca ttgtgtggct gatttctgaa
    11161 tacatccatc ctgaagagaa ttttgatgaa gaacgcaaag agatgtattc ggcccgcaag
    11221 gccctgtccc gcattgagat gaccagcatt atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg
    11341 cggaaaaact acggcacgtc atccccaatc aggacattgt agttgtcatt ttgggcttcc
    11401 tttccgccgt catagacaac gtgcctttgg tggccgcttc catggggatg tatccgatgg
    11461 atttataccc aatggatgat catatatggc aattcatcgc ctactgcgcg ggcacgggcg
    11521 gcagtatgct catcatcggt tcggctgccg gcgtagcggc tatgggcatg gagcgcatag
    11581 attttatctg gtatctcaag cattttgcct ggattgccat gctgggcttt ctggccggcg
    11641 cggctgccta tctcttgatg cttcctattt ttgaataaaa aaccacgact gttgtttcct
    11701 tctattaaaa aaatcctgcc ggcggcttta ttgctgctgg ccgtcggttg caggcaagac
    11761 ccccatcgcc tcatggcggg gcattggcag gttgttgaca tcagctcgaa ggaggaactg
    11821 ccggagtatc gcctcgatga tttccgtttt ttcttcaccg aggacgggcg ctatcagtat
    11881 ttcggcaatc tgtactatga agaggccgga gtatatacgg ttgaaggctc ctacctgcac
    11941 acaaccgata ccctgcgaca agacacaggc gaaaagacgg tgcagatact caaactcacc
    12001 aaagactcgc tcttcctgcg catggccgat gaaacgacga tgaaattggc aaaagacaaa
    12061 atcgagacgc caaaatagaa gccacgttca aacgttcgtc caaggtttac ccgccgcccg
    12121 ctaaacaatg ccggcggatg cgacgttttt gctgcgtgtt tttatcattt ttaccatctt
    12181 tgccgcctaa attgaaaatc gaatggccca gagcgtattc aaatcagatg tagaagccgt
    12241 tgatgccttg gcgcaagcct atcgcgatct ctccaaagaa atttccaaag tcattgtagg
    12301 tcaggatgat gtggtcaagg ccgtcatcat ttccctgttc agcaacgggc a
//
LOCUS       DAORLF010000085        12325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2747_length_12325_cov_1.842380, whole genome shotgun sequence.
ACCESSION   DAORLF010000085 DAORLF010000000
VERSION     DAORLF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_12325_cov_1.842380"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PK198_08945"
     CDS             complement(<1..995)
                     /locus_tag="PK198_08945"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF38904.1"
                     /translation="MRISALFMLAAIVAAGFASCNKKATTETGDLKIVPYRNGDLWGF
                     SDAKGNMLVEPKYSNAYLLDDGFGRIYQGESTGLVSPDGKVLAEPEYMYISMFKNGLA
                     AFRNNNDTLSGYLNEQGQVAIPAQFEEANDFEDGYAIVKKAGQYQLIQPDGAVIKTIG
                     KWMPLPGGDMFLSNPGFEADKGYIQVQDIGNESYSMGLIDTKGNTVLEPAYQFLSLPV
                     EGIMVAQKGEKYGLIRTDGSTVVEMKYDLIYQIGAGRYLVQQTESSYAVMDQTGKMLI
                     PAEYSSISTGPGDTYIAYKGEGVGLLDNAGKVLVPFDYNYLFASKNYLVASKGEG"
     gene            complement(1022..1603)
                     /locus_tag="PK198_08950"
     CDS             complement(1022..1603)
                     /locus_tag="PK198_08950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRF38905.1"
                     /translation="MILQNYYAHETAVIDPGAIIGEGSKIWHFCHIMSGAVIGADCNL
                     GQNVFVANEVVLGNNVKVQNNVSIYAGVVCEADVFLGPSMVFTNVYNPRSAVNRKGEY
                     LRTVVRQGATIGANATIVCGVEIGAFAFIGAGALVRKDVPPYALIVGNPGRHIGWVSE
                     HGERLYFDTQGFATCPGSGEQYQLQDGNVEKTS"
     gene            complement(1753..2322)
                     /locus_tag="PK198_08955"
     CDS             complement(1753..2322)
                     /locus_tag="PK198_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRF38906.1"
                     /translation="MEAGVKQKAEKKVQEPVNQLDEQTPRWFALYTMAKREKMVAGRL
                     TQKEITCYLPLLHLTRYYTRKIKKVELPLIRGYVFVRITKKEYVPVLETPDVVNFVKF
                     NKNLIAIPEEEIDIIRRVVGDAVQAEAEPISFEPGDEVEIIGGQLTGIRGKLVSRENK
                     REVLLELTTIGLALRVTVKEEYLRRIIKA"
     gene            complement(2372..>3069)
                     /locus_tag="PK198_08960"
     CDS             complement(2372..>3069)
                     /locus_tag="PK198_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF38907.1"
                     /translation="GSIKVAEAAKVIENTQRDLNISFMNELSIIFDRMGIDTREVLEA
                     AGTKWNFLKFYPGLVGGHCIGVDPYYLVHKSLELGYNPQVILSGRRINDGMPAFVAQR
                     LVQILIQQGKNPGACKVLVMGITFKEDVADIRNSKVADLVRELMKFSVSVHITDPHAS
                     PNEVAHEYGLSLVDQPSGDYDAVLVAVGHKDYLSHDIRWFKSVMNDKPVLVDLKGLYR
                     KPEPEEGLVYWRL"
     assembly_gap    3070..3088
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3089..>3451)
                     /locus_tag="PK198_08965"
     CDS             complement(<3089..>3451)
                     /locus_tag="PK198_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF38908.1"
                     /translation="QAHFHIVAVPTPVDEHKVPDLKPVFSASKTVASILKPGDFVVYE
                     STVYPGCTEEDCLPILEQGSGLKLGADFKIGYSPERINPGDKEHTITKILKIVSGNDA
                     EALEVVAGVYSAIIEAGI"
     assembly_gap    3452..3539
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3540..3682)
                     /locus_tag="PK198_08970"
     CDS             complement(<3540..3682)
                     /locus_tag="PK198_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF38909.1"
                     /translation="MKPEDFLTGLGRREKKISVIGLGYVGLPLALAFAKKFSVIGFDI
                     NEER"
     gene            complement(3693..4838)
                     /locus_tag="PK198_08975"
     CDS             complement(3693..4838)
                     /locus_tag="PK198_08975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRF38910.1"
                     /translation="MQTKFERPIQMVDLKGQYQKIKEDVDAGIREVMDNTAFIGGPAV
                     TRFQQNLETYLGARHVITCANGTDALQIALMALNLEPGDEVIVPAFTYVATAEVIALL
                     RLRPVMVDVDPHTFNVTAEIVEAAITPRTKAIVPVHLFGQSCDMEPIMEVAKRHKLWV
                     VEDNAQAIGADYTFGDGSRQKTGVLGHIGCTSFYPSKNLGCYGDGGAISTNDDELAAR
                     MRMIANHGQNKRYYHAMVGVNSRLDGIQAAILDVKLRHLDSYAGARQEAADMYDAAFR
                     NIPGLIVPARQHNSTHVFHQYTLIVENERREALQEHLQALGIPTMIYYPVPLYAQEAF
                     KNTGDETTFLPVTDYLCRGVLSLPMHTELDQKQLEYITGGVASFFGA"
     assembly_gap    4872..4926
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4927..>5273)
                     /locus_tag="PK198_08980"
     CDS             complement(4927..>5273)
                     /locus_tag="PK198_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF38911.1"
                     /translation="DVNVPELLEMLAFPGLHRIAEKYWRIELEELRRSFFKSAFVKAL
                     QRLIPEIRDEDLVRARSGVRAMACQKDGQLVDDFLIRRSEGVVHVLNAPSPAATASLA
                     VGDWIADVVLTG"
     assembly_gap    5274..5324
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5325..6126)
                     /gene="lhgO"
                     /locus_tag="PK198_08985"
     CDS             complement(<5325..6126)
                     /gene="lhgO"
                     /locus_tag="PK198_08985"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRF38912.1"
                     /translation="MLYDIVVIGAGIVGTATAYRLKERQPDLRLCIIDKEEGPARHQS
                     GRNSGVIHSGIYYQPGSHKALHCRKGYEALLRFCDQHEVRYDICGKIIAAVNDEEAAR
                     LSGIMEKGIANGLTGLRRLSREEALEIEPHVHCTSAILVPQTGIISYREVAEKQLELA
                     CNTGAETRFNTALTGLQRVGNETIVQTTAGDIRCKTAINCTGLYADKTAAMTHQSEGL
                     KVLPFRGEFYELVPERQYLVRNLIYPVPNPAFPFLGVHFTRMVEGGIEA"
     gene            6273..6827
                     /locus_tag="PK198_08990"
     CDS             6273..6827
                     /locus_tag="PK198_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38913.1"
                     /translation="MQKILKNIFGSHHGLDEKSVEFLISALDKNNLPGFDYIEFKQSL
                     SALNEQMTLDEATAFKSAFATASVLGLTKEKLLKTADHYRSVLNNEKQQFDSALQKQI
                     EQRVVSKQQEVQKLHKQIEEHRRAIAELEKKIAAAEDVISKADGEIAGTRERIEATRD
                     AFERTLQSVVNQVQMDIENIQRYL"
     gene            6890..7906
                     /locus_tag="PK198_08995"
     CDS             6890..7906
                     /locus_tag="PK198_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38914.1"
                     /translation="MSDMVQFNQPDMKPKSFWKRPEGITGLLFMGAMAAGAYFLITAL
                     PWKIILANTLYLAGTLVVAGALLYMVLDPKVRNLVWYMYKSVMRSITGWFVQIDPIGI
                     LKSYIESLESNLANMRKQIGNLRGQMRKIQTLMEDNSKEIAQNMKLAAAAKDKGNEQQ
                     LLLSSRKAARLKETNEKYQVLYQKMEILYRILTKMHSNSEILLEDTRGQVELKEQERK
                     AIRTSHSAMRSAMSVISGDPDQRAMFDMAMESIADDVANKVGEMERFMEVSSSFLASV
                     DLQQGVFQEEGLKMLEKWEKESTLMLMDGSTGAMPDATLNLKQLDGSKQSSGNKSYDN
                     LFES"
     assembly_gap    8078..8183
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8258..>8484
                     /locus_tag="PK198_09000"
     CDS             8258..>8484
                     /locus_tag="PK198_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38915.1"
                     /translation="MAQISIGGKPYSFALRTHTMSPLPVLMPGINIGKLEAEDLLDEQ
                     AGLAPRFGDLIEVSLNTNNSGVWETLPDGGRL"
     assembly_gap    8485..8570
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8571..>9581
                     /locus_tag="PK198_09005"
     CDS             <8571..>9581
                     /locus_tag="PK198_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF38916.1"
                     /translation="RKHVIGGFTSANNKADRIFATGLVYSDHIVLEYYEPAEVIAKRA
                     ANNVAEEASISINYVVHGYRFIEFNPNDLVKAFGSSGSCNVNTICSQGDNWRDQIKSV
                     VMLLSGGSRFCTGYLVKNTAQDCRPLLLTANHCLGTADAISSPSVNTWTFMWRYESPN
                     CTPNTDGPTNMTTSGGTVLANPGSPGSIFSSDFALIELAENPKTAGYDVYFAGFDATT
                     TAPSSATGIHHPSGDVKKISMENAALTGTSYSSTGGTATHWRVADWDSGTTEPGSSGS
                     PIFSDATKRAMGFLSGGGAACGNDLSDWYGSLGYSWNNNGASDSRRRLRDHLDPGNLA
                     TFV"
     assembly_gap    9582..9636
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9637..>10175
                     /locus_tag="PK198_09010"
     CDS             <9637..>10175
                     /locus_tag="PK198_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRF38917.1"
                     /translation="TYSSAANLNLPIPDNNPTGVSSTITVATVATVTELKCVTVNLTH
                     TWTGDLVATLTHVSSGTSVQLFRRLNATTPTAVGESSNLSGAYTFCATGASFATAGNG
                     GLTDYVIPPGTYAQSSASLQLTPAQNTNTFANFVGLALNGQWRLTVSDYSADDIGTLS
                     NWSFEACSDAPTCPTFSGAP"
     assembly_gap    10176..10292
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10328..>10642)
                     /locus_tag="PK198_09015"
     CDS             complement(10328..>10642)
                     /locus_tag="PK198_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38918.1"
                     /translation="VSKLAAEQVLSVTLVPSPVKVYHTPAVVCTTPHGAKEPGSAVAL
                     MLELEMVIPQLIGVALAHKSLAGAQEPGMVVTAVDSALTVLASLSQAQRVLMLCAGPV
                     WL"
     assembly_gap    10643..10845
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10846..11523
                     /locus_tag="PK198_09020"
     CDS             <10846..11523
                     /locus_tag="PK198_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38919.1"
                     /translation="PANVSISNSTCGSGCTLTGGSITAPAGTPCPAVSTLQYNVNNSG
                     WTTSLPSYNQSGPAQTIRTRCACNNDGNNVSAESNPVSTIPGTLANPVVPANGSAIVA
                     CPALATQPTPPTVMGCDGSIITPTGPVISNSPNPVTCEGTRTYTWTYSCGGTSSIWSF
                     VYTIERNPFTVRSSHSQLSVVGHPAHAAYCDEQLRRGADAERPRSNQRSGSDCLCRHP
                     DVCLDLH"
     gene            11435..>12325
                     /locus_tag="PK198_09025"
     CDS             11435..>12325
                     /locus_tag="PK198_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38920.1"
                     /translation="MLTPSGPAVTNVPDPIVCAGTRTYAWTYTDCEGNTAVWSFVYTI
                     IDNTPPTATCHNQTITFNGQESITLNANDLVTVSDNCGTPSITLSPSFITCQQVGQIV
                     PVVITVKDASNNTVTCTANVTVGGLPCGWSQNPNGVNCANGNSIAYNAATGVYTATST
                     GCFYGPPFTGDATAFAQRTLCGNGSITALVTGINPLAGGWAGIVMRENNTAGAKKAQL
                     MTNLGSDHRREFRIATNGSAQVQQFPSQNRYWLRITRVGNQFTMFVSSNGVNWFPAGA
                     QNIVMPSCIQMGLVATNYTAN"
BASE COUNT         2689 a         3224 c         2978 g         2654 t
ORIGIN      
        1 ttgccttccc ccttcgaggc gacaaggtag tttttggatg cgaacaggta gttatagtca
       61 aaaggtacca ataccttgcc ggcattgtcc aaaagcccca cgccttcacc tttgtaggca
      121 atgtaagtat cgccaggccc cgtactgatg ctgctgtatt cggcaggaat gagcattttg
      181 ccggtctgat ccataacagc ataagaactc tctgtttgct gaaccaaata ccggcctgcg
      241 ccgatctgat aaattaaatc gtacttcatc tccaccaccg tactgccgtc ggtgcggatg
      301 aggccgtatt tctcgccctt ttgcgctacc ataattcctt ccaccgggag cgataaaaat
      361 tgataggccg gctccagtac ggtattgcct ttggtgtcta tcagccccat ggaataagac
      421 tcgttcccga tgtcctgaac ctgtatgtaa cctttatcag cctcaaagcc ggggttgctc
      481 aggaacatat cgccaccggg aagcggcatc cacttgccaa tggttttgat gaccgcgccg
      541 tcgggttgga tgagttgata ttggccggct ttttttacaa tggcataccc atcttcaaaa
      601 tcgttggctt cttcaaattg ggccggaatg gccacctgcc cctgctcatt gaggtagccg
      661 gagagtgtgt cgttgttatt gcgaaaagca gccaatccat ttttgaacat ggaaatgtac
      721 atgtattccg gctctgccag cactttgccg tcgggcgata ccagcccggt ggattccccc
      781 tgataaattc ggccgaaacc gtcgtccagc aggtaagcat tgctgtattt aggctccacc
      841 aacatgttgc ctttggcatc cgaaaaaccc cagaggtcgc cgttgcgata agggactatt
      901 ttcagatcgc cggtttcggt agtcgctttt ttgttgcagg aagcaaaacc tgcggccacg
      961 atggcggcca gcatgaaaag tgctgaaatt ctcatagagt atggtgcttt tgaatagatg
     1021 actatgatgt tttttctacg ttgccgtcct gtagctggta ctgctcgccg ctgccggggc
     1081 atgttgcaaa gccttgtgta tcaaagtaca atcgttcgcc gtgttcactc acccagccga
     1141 tgtggcggcc cggattgcct acgatgagcg cgtaaggcgg cacgtctttg cgcaccagcg
     1201 ctcccgcccc gatgaaggca aaggcgccga tttctacccc gcaaacgatg gtggcattgg
     1261 ccccgatggt agctccctgc cggacgacgg tgcgcaggta ttcgcccttg cggtttacgg
     1321 cgctgcgcgg gttgtaaaca ttggtgaaaa ccatcgaagg ccccaggaaa acatcggcct
     1381 cgcaaacgac gccggcataa atggagacgt tgttttgcac tttcacgttg ttgcccaata
     1441 cgacctcatt ggctacaaat acattttgcc ccagattgca atctgctccg atgactgctc
     1501 ccgacataat gtgacagaaa tgccagattt tgctgccctc gccgatgatg gcgccggggt
     1561 ctatcacggc agtttcgtgc gcgtagtagt tttgtaagat catgtaaggg tgactcagat
     1621 ggctgtgtgt cgcgagagga gactcgaagc tcgaagctcg taactcgcag ctataaaaaa
     1681 cggcttcgcc ctcgaagagt cgcagggact ttcttgcttg aagcaacaga tgtgaagaaa
     1741 atcagccaat aatcaggctt ttataatcct gcgaagatac tcttctttga ccgttacccg
     1801 cagcgccagg ccgatggtcg tcaactcaag caatacctcg cgtttgtttt ctcttgatac
     1861 caatttgccc ctaatgccgg tgagttgtcc gccgatgatt tccacctcgt cgccgggttc
     1921 aaacgaaatc ggctctgctt ccgcctgcac cgcatcgccc accacccgcc ggatgatgtc
     1981 aatttcttct tcgggtatgg caatcaggtt tttattaaac ttgacaaagt taaccacgtc
     2041 cggcgtttcc agaacgggta catattcctt ttttgtaatg cgcacaaaga catagccccg
     2101 gatgagcggc agctctacct tcttgatttt gcgcgtgtag tatctcgtca ggtgcagcaa
     2161 gggcaggtaa caggtgatct ctttttgcgt caggcgaccg gccaccatct tctcgcgttt
     2221 ggccatggtg tataaggcaa accatctcgg cgtctgttca tccaattgat tgacaggctc
     2281 ctgcactttt ttttccgcct tctgttttac acctgcttcc atccgaaacc catttttcac
     2341 tcactcactc acccctcaca cactcacccc tttacaacct ccaatacacc aatccctcct
     2401 caggctccgg tttcctgtac agccccttca aatcaaccag aaccggcttg tcgttcatca
     2461 ccgatttgaa ccaccgaata tcgtgagaca ggtagtcttt atgcccgacg gccaccaaaa
     2521 cggcgtcgta atcgccggag ggctgatcta cgagcgaaag gccgtactcg tgcgccacct
     2581 cattgggcga tgcgtgcgga tcggtgatgt gtacagagac ggaaaatttc atcagttcgc
     2641 gcaccagatc ggctactttg gagttgcgga tgtcggccac atcttcttta aaagtaatgc
     2701 ccatgaccag cactttgcag gcgcctggat ttttgccctg ttggatgagt atttgtacca
     2761 aacgctgcgc cacaaaagcc ggcattccgt cgttgatgcg ccgtccgctg aggatgacct
     2821 gcgggttgta gccgagttct aagctcttgt gtaccaagta gtaagggtcc acgccgatgc
     2881 aatgtccacc taccaaacct gggtaaaatt tgagaaaatt ccatttggta ccggcggctt
     2941 ccaacacctc tctggtatca atgcccatgc ggtcgaagat gatggaaagc tcgttcataa
     3001 acgagatatt gaggtcgcgc tgggtatttt cgatgacctt ggcggcctcg gccaccttga
     3061 tgctgcctgn nnnnnnnnnn nnnnnnnnaa aatccccgcc tcaatgatgg cgctatacac
     3121 gccggctacc acttcgagcg cctcagcgtc gttgccggaa acaattttga gaatcttggt
     3181 aatggtgtgc tctttgtcgc cgggattgat gcgttccggc gagtaaccga ttttgaaatc
     3241 ggcgccgagt ttcagccccg aaccctgctc cagaataggc aggcaatcct cttccgtaca
     3301 accagggtaa accgttgact catacaccac aaagtcgccg ggcttgagaa tggaggccac
     3361 tgttttagag gcggaaaaca ctggtttcag atcgggaact ttgtgctcgt caacgggagt
     3421 aggaacggca acgatgtgaa aatgagcttg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3541 gctcctcgtt gatgtcgaag ccgatgacgc tgaatttttt ggcgaaagcc aaggccaatg
     3601 gaagccccac atatcccagc ccgatgacgg aaattttctt ttctctgcgg ccaagcccgg
     3661 tcaaaaagtc ttctggtttc atggaattca tttcaagcac caaaaaaaga agctactccg
     3721 ccggtaatgt attccaattg tttttgatcc aattcggtat gcatgggcaa cgaaagcact
     3781 ccgcgacaaa ggtagtcagt cacgggtaaa aaagtagttt catcgccggt atttttaaac
     3841 gcttcctgag catacagcgg cacaggatag tagatcatgg tggggattcc gagtgcctgc
     3901 aagtgttcct gaagggcttc gcgccgctca ttttcaacga tcaatgtgta ttgatgaaat
     3961 acatgtgtgg agttgtgctg ccgcgccggc acgatgagtc caggaatgtt tcggaaagcg
     4021 gcgtcataca tgtcggcggc ctcctgcctg gctccggcgt agctgtccag atgcctcaat
     4081 tttacatcca gaatggcggc ctgaatgccg tcgagccgcg agttgacgcc caccatagcg
     4141 tggtagtagc gtttgttttg gccatgattg gcaatcatgc gcatcctggc ggccagttcg
     4201 tcgtcgttgg tgctgatggc gccgccgtcg ccatagcaac ctaagttttt ggatgggtaa
     4261 aatgaggtac agccgatatg gcccaatacg cctgtttttt ggcggctccc atcgccgaaa
     4321 gtgtagtctg ctccaatggc ctgggcgttg tcttccacca cccaaagttt atgacgcttc
     4381 gccacttcca tgatcggctc catgtcgcag ctttggccaa agaggtgtac cggcacaatg
     4441 gctttggtgc gcggagtgat ggccgcttct acgatttcgg cagtcacatt gaaggtgtgc
     4501 gggtccacgt ccaccatcac cgggcgaagg cgcagcaatg caatgacctc agccgtagcc
     4561 acataggtga aagcgggcac gatgacttcg tcgccgggtt cgaggttcaa agccatgagg
     4621 gctatctgga gggcgtcggt gccgttggcg caggtgatga cgtgccgggc gccgaggtag
     4681 gtttccaaat tttgctgaaa gcgggtcacg gccgggcctc ctataaaggc ggtgttgtcc
     4741 atcacttcgc ggatgccggc gtccacgtct tctttgattt tttggtattg gcccttcaaa
     4801 tccaccatct ggatggggcg ctcaaatttg gtttgcatat tttgacaagt tgattttttg
     4861 attttgcaat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnctac cctgtcagaa ccacatccgc aatccaatcg cctactgcga gcgatgcggt
     4981 ggctgccggc gagggcgcgt tgagtacatg cactacgcct tcggagcgcc ggatgaggaa
     5041 atcgtctacc aattggccgt ccttttgaca ggccattgcc cgcacgcccg atctggcccg
     5101 cacgaggtct tcatcgcgta tttccggtat gaggcgctgc aaggctttga caaatgcgct
     5161 tttgaaaaaa ctccgccgca gttcttccaa ctcaatgcgc caatactttt cagcaatgcg
     5221 atgcaagccc ggaaaggcca gcatttccaa tagttccggc acatttacat cccnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggctt cgatgccgcc
     5341 ctcgaccatg cgggtaaaat gcacgcccaa aaaggggaag gccggattgg gtacgggata
     5401 aatgagattt cttaccaaat attgccgctc cggcacgagc tcgtaaaatt cgccccggaa
     5461 gggcagcact ttcagcccct cgctttggtg cgtcatggca gcggttttat ctgcgtagag
     5521 gccggtgcag ttgatggcgg ttttacagcg tatatcaccg gcagtggttt gtacaatggt
     5581 ttcgttacca actctttgca agccggtcag cgcggtgttg aatctcgttt ctgcacccgt
     5641 attgcaggcc aattccaatt gtttttcggc cacttcgcgg tagctgatga tgccggtttg
     5701 cggtacgagt atggcggaag tgcaatgaac atggggttct atttccagcg cttcttcccg
     5761 gctcaatctg cgcaggccgg tgaggccgtt ggcgatgcct ttttccataa tgcccgacaa
     5821 acgcgccgcc tcttcatcgt tcaccgcagc gatgattttg ccgcagatgt cgtaccgaac
     5881 ctcatgttga tcacaaaaac ggaggagggc ttcgtagcct ttgcggcaat ggagggcttt
     5941 gtggctgccg ggttggtaat aaatgccgga gtgaatgacg ccgctgttgc gccctgattg
     6001 gtggcgggca ggtccttctt ctttgtcaat aatgcaaagc cgcaggtctg gctgccgttc
     6061 ttttagcctg tatgcagtgg ccgttcccac aattcccgct ccgatgacaa caatatcgta
     6121 tagcaagcct ctttgagttt gacggggcga agttacacgg ttcgggcgaa caagcaaaaa
     6181 gaaaactctg gtcggctgaa tgtgaaaaac catcgaaaat tgcccgcctt atgcgccggt
     6241 ttgcgttaat ttgcaccgtc acaatactta ttatgcaaaa gattctgaaa aacattttcg
     6301 gcagccatca cggtttggat gaaaaaagcg tggagtttct gatttccgct ttggacaaaa
     6361 acaacttgcc cggcttcgac tacatcgagt tcaagcagtc gctctcggcg ctcaacgaac
     6421 aaatgacatt agacgaagcc acggctttca agtcggcttt cgcaacagct tctgtattgg
     6481 gcctcaccaa agaaaaactg ctcaaaaccg ccgatcatta tcgcagcgtg ctgaacaacg
     6541 aaaagcaaca gttcgattcg gccctgcaaa aacagatcga acagcgggtg gtgagcaagc
     6601 aacaggaggt gcagaaattg cacaaacaaa ttgaggagca ccgcagggcc attgccgaat
     6661 tggagaaaaa aattgccgcc gccgaggatg ttatttccaa agccgacggc gaaattgccg
     6721 gcaccagaga acgtatcgaa gccacccgcg acgccttcga acgcacactg caaagcgttg
     6781 tcaatcaggt gcaaatggac attgaaaaca tccagcgtta tttgtaatta caaagagctg
     6841 ccaagccttt gatcatttct ttttcaccca ataaaaaaat tatcgagcta tgtctgatat
     6901 ggttcaattc aatcaacccg acatgaaacc caagtctttt tggaagcgac cggaaggtat
     6961 taccgggctt ctcttcatgg gcgctatggc tgccggggct tattttctga ttacggcctt
     7021 gccttggaag attattctcg ccaatacgct ctacttagcc ggcacattgg tagtagcggg
     7081 cgccttgctc tacatggtac tcgatcccaa agtgcggaac ttggtgtggt acatgtacaa
     7141 aagcgtaatg cgctccatca ccggctggtt tgtacagata gaccccatcg gcatactgaa
     7201 aagctatatt gaaagcttgg aaagcaatct ggccaacatg cgcaaacaga tcggcaacct
     7261 gcggggccaa atgcgcaaaa tccagacact gatggaggac aacagcaaag agatagcgca
     7321 aaacatgaag ttggccgctg ccgccaaaga caagggcaac gaacaacaat tgctgctctc
     7381 atcccgcaaa gcagcccgcc tcaaagagac caacgagaag tatcaggtgc tgtatcaaaa
     7441 aatggagata ctgtaccgga ttctgaccaa aatgcactcc aattccgaga ttttgctcga
     7501 agacacccgc ggtcaggtag agctgaaaga gcaggaacgc aaggccatcc gcacctcgca
     7561 ctcagccatg cgctcggcca tgagcgtcat ttccggcgac cccgatcagc gcgccatgtt
     7621 cgatatggca atggaatcca ttgccgacga tgtggccaac aaagtgggcg aaatggagcg
     7681 cttcatggag gtatcctctt cgttcctggc atccgttgac ctgcaacagg gcgtttttca
     7741 ggaggaagga ctcaagatgc tggaaaaatg ggaaaaagaa agcaccctca tgctcatgga
     7801 cggctcaacc ggcgccatgc ccgatgctac tttgaacctc aagcagttgg acggctccaa
     7861 acagtcgtcg ggcaataaga gctacgacaa cctgtttgaa tcctgataag gggcgataaa
     7921 ataccaagga ctgcgaaagc aggccaacaa gaggaatcat cccgaatttt gaagagattc
     7981 aaagttcggg attttttttg cctgataaaa atgaaatcaa tgaaggggaa aggttcgacg
     8041 catatatttt tgaccgcttg aggtgcgcga aaaagaannn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnaacttat gacgagtaaa tcactaaccc atctcgtgtt
     8221 cggggtcttg tttggctgtt tgttcctgca aacggccatg gcccaaatca gcatcggcgg
     8281 caagccgtac agctttgccc tccgcaccca taccatgtca ccgctgcctg tgctgatgcc
     8341 gggcatcaat atcggcaagt tggaggcgga agacctgctg gacgaacaag ccggtctggc
     8401 gccccgtttc ggcgatctta ttgaggtcag tctcaacaca aacaattccg gcgtatggga
     8461 aacactgccc gacggcggac gcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgcaaacac
     8581 gtcatcggcg gctttacatc ggccaacaac aaagccgacc ggattttcgc cacgggcctg
     8641 gtgtacagcg accacattgt acttgaatat tacgaacccg cggaggtgat tgccaaaaga
     8701 gcagccaaca acgtagctga ggaagcctcc atcagcatca attatgtagt gcatggctat
     8761 cgttttatag agttcaatcc gaatgactta gtgaaagcgt tcggcagttc cggctcctgc
     8821 aatgtcaaca cgatatgttc gcaaggcgac aactggcgcg atcagatcaa gtcggtagtg
     8881 atgctgcttt ccggaggatc caggttttgc acaggctatt tggtcaaaaa tacagcgcag
     8941 gattgccgcc ccttgttgtt gacggccaac cactgtcttg gcactgccga cgccatcagc
     9001 agtcccagcg taaacacctg gacgtttatg tggcgctacg aaagccccaa ttgtacgccc
     9061 aacaccgatg gccccaccaa catgaccacc agcggcggta cggtactggc caaccccggc
     9121 agcccgggca gcattttcag tagcgacttt gctttgattg aactggccga aaaccccaaa
     9181 actgcgggct atgatgtcta tttcgcaggc ttcgacgcca ccactacggc gccaagcagc
     9241 gctacgggca tccaccaccc cagtggcgac gtaaagaaaa tttcgatgga aaacgccgcg
     9301 cttaccggca cttcctacag cagcaccggc ggcacagcca cccactggcg agtggccgac
     9361 tgggacagcg gcacgaccga acccggctct tccggctcgc ctatctttag cgacgctacc
     9421 aaaagagcca tgggcttttt gtcgggcggc ggcgctgcct gcgggaacga cttgtcggat
     9481 tggtacggca gcctgggcta tagctggaac aacaacggcg ccagcgacag ccgccgcagg
     9541 ttgcgcgacc atctcgaccc cggcaactta gctacctttg tnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct actcctcggc agccaacctc
     9661 aatctcccaa tcccggacaa taacccgacg ggcgtcagct ctaccattac ggttgctacc
     9721 gtagccacgg ttactgagtt gaagtgcgtg acggtcaatt tgacccacac ttggacaggc
     9781 gacctcgtag ctaccctgac acacgtcagc agcggcacct cggtgcagtt gttccgtcgt
     9841 ctgaatgcca ctactcctac cgctgtcggc gaatcttcca acttgtcggg tgcttatact
     9901 ttttgcgcta cgggcgccag ctttgcaacg gccggaaatg gcggcttaac agactatgtt
     9961 attcctccgg gcacatacgc acaatccagc gccagcctgc aactcactcc ggcccaaaac
    10021 accaacacct ttgccaattt cgtagggctt gccttaaatg gccaatggcg tttgaccgtt
    10081 tctgactaca gcgctgatga tataggcaca ttgtctaatt ggagctttga ggcttgctca
    10141 gatgcgccca cttgccccac cttcagtggc gcgccnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttgataa taccggctgg acgactacct
    10321 tgcccagtta caaccaaacc ggcccggcgc aaagcatcaa aacccgctgc gcctgcgaca
    10381 gtgatgccaa taccgtgagc gcagaatcca ccgctgtgac gaccatacct ggctcttgtg
    10441 cgccggccaa tgatttgtgc gccaatgcga cgcctatcag ctgcggtatt accatttcaa
    10501 gctctaacat caatgcgact gcagaaccag gctctttcgc accatgcggt gtcgtgcaaa
    10561 ccaccgccgg cgtatggtac accttcacgg gcgatggaac taacgtaacg ctcagtacct
    10621 gctctgcggc taatttcgat acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc caatgtgagc
    10861 atcagcaaca gcacttgcgg cagcggttgt acgctcacgg gcggcagcat cacagcgcct
    10921 gcaggcacgc cttgtccggc agtctctacg ctgcaataca atgtcaacaa cagcggctgg
    10981 acgacgagct tgccgagcta caaccagtca ggcccggctc aaacgattcg cacacgctgc
    11041 gcctgcaata acgacggcaa caacgtcagc gcggagtcca atccggtgag tactattccc
    11101 ggcacattgg ccaacccggt agtgcctgcc aatggttcgg ctatcgtagc ctgcccggcc
    11161 ttggccacac agcccacgcc gcctaccgta atgggttgcg acggctcgat catcacgccc
    11221 accggcccgg tcatcagcaa cagtcccaac cccgttacct gcgagggtac gcggacatac
    11281 acctggacat actcctgcgg cggcacttca tctatctgga gctttgtcta taccatcgag
    11341 cgcaatccgt ttaccgttcg gagcagccac agtcaactct ccgtcgttgg ccacccagcc
    11401 cacgccgcct actgtgacga gcaactgcgg cgaggtgctg acgccgagcg gccccgcagt
    11461 aaccaacgtt ccggatccga ttgtctgtgc aggcacccgg acgtatgcct ggacttacac
    11521 tgattgcgag ggcaataccg ctgtatggag ctttgtttat accatcatag acaacacgcc
    11581 tccgacggcc acttgccaca accagacgat cactttcaac ggacaggaga gcatcacatt
    11641 gaatgccaat gatctggtaa cagtctccga caactgcggc acgccgagca ttacactgag
    11701 tccgtcgttc atcacctgcc agcaggtggg gcagatcgtg ccggtcgtca ttaccgtgaa
    11761 ggatgcgagc aacaatacgg tcacttgcac tgccaacgtg acggtagggg gcctgccctg
    11821 cggctggagc caaaacccga acggcgtgaa ctgcgccaat ggcaacagca ttgcctataa
    11881 tgcagccaca ggcgtttaca cagctaccag tacaggctgc ttttacgggc cgcccttcac
    11941 cggcgatgcc acggctttcg ctcagcgtac gctctgcggc aacggcagca tcacggcatt
    12001 ggtgacgggc atcaaccccc tggccggcgg ctgggcgggt atcgtgatgc gcgaaaacaa
    12061 cacagcgggc gcgaagaaag cgcagttgat gacgaacttg ggcagcgatc accggcggga
    12121 gttccgcatt gctaccaatg gctctgcgca ggtgcagcag ttcccgagcc agaatcgcta
    12181 ttggctgcgc atcacgcgcg tgggcaatca gttcaccatg tttgtgtctt ccaatggagt
    12241 caactggttt ccggcaggcg cgcaaaacat agtgatgccg tcttgtattc agatgggctt
    12301 ggtagcgacc aactacaccg ccaac
//
LOCUS       DAORLF010000086        12321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2748_length_12321_cov_2.132562, whole genome shotgun sequence.
ACCESSION   DAORLF010000086 DAORLF010000000
VERSION     DAORLF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_12321_cov_2.132562"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK198_09030"
     CDS             complement(<1..208)
                     /locus_tag="PK198_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38921.1"
                     /translation="MNLLDFLDRFRKTPAFSVREVEQLYPGFERENLLNWQKKGHIIR
                     VRNGWYSVKGRIATEEQLYWVANKI"
     assembly_gap    291..314
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(488..802)
                     /locus_tag="PK198_09035"
     CDS             complement(488..802)
                     /locus_tag="PK198_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38922.1"
                     /translation="MSPTFWKGYSNRERHAEISNISSIISQFGDIVDFKLFSDVSISI
                     TMEVSAQNAGALLEALQDCIQIVEVTPLPAASAREIIIYLNLTFSVATGNLKIEVPQV
                     PG"
     gene            complement(831..>1386)
                     /locus_tag="PK198_09040"
     CDS             complement(831..>1386)
                     /locus_tag="PK198_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF38923.1"
                     /translation="RLKQWRDEMMEILDMEAYEVMSVKALIDIAEILPANLTALKKVK
                     GIGEGKAKRFGSQILEIVNRYCVEKGLAGAQLALPDPEPPKPKVNTKQVSYDLFRAGK
                     SIADIAQERGFTAGTIEGHLAHYIGTGELDISELIEPAKLKVLSAYLKEHYQGELASI
                     KAHFGDEVSFGELRMVAEYLKREG"
     assembly_gap    1387..1424
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1425..>2688)
                     /locus_tag="PK198_09045"
     CDS             complement(<1425..>2688)
                     /locus_tag="PK198_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRF38924.1"
                     /translation="ISLLNKVRDNQLTAEVLDQLNSRYQPGFEAPEGQAYITLTSHNA
                     SAQAINAEKLAGIKGELHRFKAEITGEFPEHAYPTDEVLELKTGAQVMFVKNDPNREK
                     RFYNGKIGRITRIGKDEIIVQCPDDEEPITVTTTDWNNVKYALDEKTKEVSETVIGSF
                     VQFPLRLAWAITIHKSQGLTFERAIIDAQAAFAHGQVYVALSRCKSFEGIVLRTPIAY
                     SSVKTDSTVRNFSAESEKNAPDEEHLRQSKREYQASLLMELFDLSGIKSKMEHANRTF
                     LSHEGSLAKDLVQPFQQLYMAAETDLFPVAAKFRGQMRGYFAQPEMPEDNAALQERVG
                     KACVWFLEKIGTGLLETVKKMPVITDNKAVKKVADEAMENLQRELFVKKSCLDACLNS
                     FSARTYLRAKADASMDFKPVSPMSAGFAG"
     assembly_gap    2689..2726
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2727..3288)
                     /locus_tag="PK198_09050"
     CDS             complement(<2727..3288)
                     /locus_tag="PK198_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF38925.1"
                     /translation="MNYQSNPQLELAFDYVGSTNKNIFLTGKAGTGKTTFLQNLKKDS
                     IKRMAVAAPTGVAAINAGGMTLHSLFQLPFGLFLPEHSRDASRQRRFTREKIRLIQSL
                     DLLVIDEISMVRADLLDAVDDVLRRYKDRSLPFGGVQLLMIGDLHQLPPVVKEDEWEM
                     MRPYYETPYFFSSRALALTETVAIELK"
     gene            3551..>5890
                     /locus_tag="PK198_09055"
     CDS             3551..>5890
                     /locus_tag="PK198_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38926.1"
                     /translation="MMKKALLLFATALWCSFSASAQQAGTLDVSFGQQGIVETGFGAG
                     LQAMAMEILADGKIMVVGSHAENDVRSLIVLQYLPDGAPDILFGNAGKRIVALPPLGY
                     LMFVKINPADGSLLLGSGGVNSNLAITRYSPAGEWDTFFGNGGIVTEPFNYVNTYADA
                     LFLPDGKILVGAYGYAFSPSHLKGYLIRFNADGTLDTSFGEEGYAPIFMPGNQILQVL
                     ALQADGRILAAGHTHPGTTSSLPWRTFVARLHPDGNIDEDFGTGGSILLGIGVPASIL
                     LRPDGKIIVGGGLESLDNPGLLYIEQLLANGIPDISFGTSGRVSLTTQRPIKARPKLE
                     RTVSGQLVLYTILQYQLGLWRFHDNGSPDTSFGNQGLLTADFDNGQMSSNIVILHQQD
                     RLLITGTLHRDDILLGRLLHNGSLDTNFGEGGFSITDLGSHRIYAGPIAAFPDGRIQT
                     SALVGNRFVSMRYLPNGNLDHAYGQSGLASVRLPHHDYVYDISFSIDAQGGIAALISI
                     EEENKSIYIARLRPDGTPEQGFGTDGLLRVVFGLGDSYGGSVSILENGKILIAGTFAN
                     TNGIHFFAMSQFLPDGQADQSFGNGGVAISQTANNAFCSNMAVLPDGKILLTGNQEPV
                     PSRLISARYFSNGFIDASFGSFGRVETAIGQQLVSSQNISLQSDGKILIAGSVEGKAI
                     LVRYLPDGTLDSEFGNAGVFISDNLTGWKTQVRVQLDGTLFLVGGQRTNNIYTLTVMQ
                     LNAQGMPEPNFGTDGFAVLPTARDMYLNGSELLSDHKLVL"
     assembly_gap    5891..6005
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6006..6239
                     /locus_tag="PK198_09060"
     CDS             <6006..6239
                     /locus_tag="PK198_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38927.1"
                     /translation="PADEMLYVEYTLPRAGRVHIRLFDVYGRPLQTLLQPDQRAEGPQ
                     HEQFRLPDALPTGVYWLSLEADQKSAAVKVVRR"
     gene            complement(6353..6964)
                     /locus_tag="PK198_09065"
     CDS             complement(6353..6964)
                     /locus_tag="PK198_09065"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRF38928.1"
                     /translation="MLIYGSKATQIASKSLSDKCTNCGAENSVQMAVFQKYAHVFWIP
                     FFPIGKTGVTQCAHCKQVLEKKEFSSSLLSTYETLISDSKTPRWTFSGLAVLTLLIIL
                     VSISNAQNDKKNAQLILAPKKGDIYEIRNDYEHYTLYKIDRVAGDTVFILMNEYESNK
                     VTGLNTLKGKGDAAFVDTPIPLFKSDLKSMFDAGKIMNIDRKQ"
     assembly_gap    7036..7053
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7099..8352)
                     /locus_tag="PK198_09070"
     CDS             complement(7099..8352)
                     /locus_tag="PK198_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRF38929.1"
                     /translation="MSGFSEIKKGLSGLETLERELNLKQLQINRLLGITQAINNNVSA
                     DGLYDMYKSFLGWELGVKKMALYVHEDDEDWRCAGAIGISDELLKTDIRPLLPRYTRL
                     KNVEDNDNPLIREFDVVIPVQHKELPIAYVFIGGFKEDDDMYGKVQFITAITNVIAVA
                     IENKRLFKRQLEQERLKHEMELAGEMQRMLIPKSLPTNHHYDLAGVYRPQLGVGGDYF
                     DYMEFDDGKLVFCVGDISGKGVAAALLMANFQANFHTLIAKRESLDDFVRDLNTTVNR
                     ITGGDRFITFFIAEYDPATRSLQYVNAGHNPPLLVSDDQFRFLDKGCTILGSFTALPK
                     VEIGRLTVEGNAVIMAFTDGLTDLRDPSGQFLDEAMLHHFARANYLLPPMEFNRLLLE
                     QIERFKGNQSYPDDFTLLTCRVFGG"
     gene            8568..10130
                     /locus_tag="PK198_09075"
     CDS             8568..10130
                     /locus_tag="PK198_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRF38930.1"
                     /translation="MKNYTLLATLLWLFFSVQTMAQPVPPKREFRGVWVATVANIDYP
                     KAPDQRSVALKEQWIQLVEMYKNMGLNAVIVQVRPAGDAIYPTEMAPWSKYLTGRQGL
                     PPADTTFDPLAFMIEEAHRNGMEFHAWFNPYRATMDLDTLSLSSMHLFNKRRDWMLRY
                     GNRFLLNPALPEVREHIAQVVGEVVQKYDVDAVHFDDYFYPYKVAEEVFPDSLEYLVL
                     GKSFANIDDWRRSNVDALVQLVSEKIRDTKPHVQFGISPFGVWRNRDKDPVMGSDTRA
                     GLTSYDDLYADVLKWSRLGWIDYVAPQLYWHIGFTPADHYTLLKWWERNAYGRLLHIG
                     HGAYKVANNQEVAWYDPAEMSRQIDLNRRNLTASGSIYFSSKSLVNNPLGVRDSISHY
                     YARPSLLPIPPDKAQKAFEAPQLLQVKSKNIQPLLRWRPNRLDRKNPPLYYVVYRFEG
                     NTFGDFENARYIMTITPRGLTQKTFEFHDATVLPDKTYTYTVTAFNRTHVESKAAPGR
                     TVYIGQFKVQRK"
     gene            10266..10853
                     /locus_tag="PK198_09080"
     CDS             10266..10853
                     /locus_tag="PK198_09080"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRF38931.1"
                     /translation="MFLEPHFKGQRSGWIEVICGSMFSGKTEELIRRLKRAKIANQHT
                     EIFKPRIDTRYDDTRVVSHDANSVLSMPVDHSSKLLQLADGFEVVGIDEAQFFDMGLT
                     EVCQELALRGIRVIVAGLDMDYRGKPFGPMPNLLAVAEYITKVHAICPHCGNLATHSY
                     RLSQDNADIVLGEKDLYEPRCRTCYSMGNILKLHT"
     gene            complement(10896..>11103)
                     /locus_tag="PK198_09085"
     CDS             complement(10896..>11103)
                     /locus_tag="PK198_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38932.1"
                     /translation="PDRPPPTRKKSLSVPSEPAGFLFAPLYLAGKCYISRKISSAFIT
                     KPSTTGSALQSLAPLHSSTVSGAR"
     assembly_gap    11104..11146
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11154..>11566)
                     /locus_tag="PK198_09090"
     CDS             complement(11154..>11566)
                     /locus_tag="PK198_09090"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38933.1"
                     /translation="IATNYTANSTVTATFAGVSFTGSNSGFSTPSTPHALTPHLSPLT
                     PEFDVYPNPTSGDLNVDLTQYVGRSVRLELYSLEGKLLQFSEIEEVQTTVEHLSLSQY
                     ANGMYFVKVSDVTRDGISRERLPAVTKRVVLQRL"
     assembly_gap    11567..11673
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11674..>11903)
                     /locus_tag="PK198_09095"
     CDS             complement(<11674..>11903)
                     /locus_tag="PK198_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38934.1"
                     /translation="DATSFAQRTLCGNGSITAQVTSISGALGWAGLVMRESNAAGAKK
                     AQLLTNLSSLHRREFRTTTNGAAALQQSASNG"
     assembly_gap    11904..12023
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12024..>12321)
                     /locus_tag="PK198_09100"
     CDS             complement(<12024..>12321)
                     /locus_tag="PK198_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF38935.1"
                     /translation="DINGLTNTCTFTVTKVDNTAPTVQCFNQTLNFNGEESISLQSNA
                     LASASDNCGVQSIGLSPSTISSAQVGQIVPVTVTATDINGNTSTCTAEVTVSGLP"
BASE COUNT         2667 a         3010 c         3170 g         2971 t
ORIGIN      
        1 atattttatt ggccacccaa tacagttgct cttcggtggc aatgcggcct tttacactgt
       61 accagccatt ccgaacacga ataatatgac cttttttttg ccagttgagg aggttttccc
      121 gctcaaaacc ggggtaaagc tgttctacct cccggacaga aaatgcgggc gtttttctaa
      181 atctatccaa aaagtcaagt aagttcattt ctatttatcc taaaaattta ggttttcagg
      241 aaacaaaaat acgattttta tgaatagcag gcgcagccgg gaaagaaaat nnnnnnnnnn
      301 nnnnnnnnnn nnnncattct gaagccagtg ttatcgcgag gcacgatact tcacaaaagc
      361 ctcttcatcg ggcaagccat ccaaaattat ggctactgca ccagaaatgg cgtgttccaa
      421 agctccaatg tgcggacttg cccctggctg atcaaatgat ttcttttatt cattggtcag
      481 acattgttta ccccggcact tgcggtactt ctatttttag atttccagta gctacagaga
      541 acgttaagtt gagataaatg atgatttccc gcgccgatgc agccggcaag ggagttacct
      601 cgactatctg gatacagtct tgcaaggctt ccagcaatgc gccggcattt tgtgccgaaa
      661 cttccatcgt tattgaaatg gagacatctg aaaacaactt gaaatcaacg atgtctccaa
      721 actgcgatat gatgctgctg atgttgctga tttcagcgtg gcgttcccga ttgctgtatc
      781 ctttccagaa tgttggactc acgatttgat attttatgct gagtacatcg ctacccctcc
      841 cgcttcaaat actctgccac catgcgcagt tcgccgaagc tgacttcgtc gccgaaatgg
      901 gctttgatgg aagccagctc gccctgataa tgctccttca aataagccga taaaaccttc
      961 aatttggccg gttcgatcag ttcggagatg tcgagttccc ccgtgccgat gtagtgtgca
     1021 agatgcccct cgatggtacc cgcagtgaag ccgcgctcct gtgcaatatc ggcgatgctt
     1081 ttgcctgccc ggaacaggtc gtaactcacc tgtttggtat tcactttcgg tttgggcggt
     1141 tcaggatcgg gcagggcgag ctgcgcgccg gccaggcctt tttccacaca atagcgattc
     1201 acgatttcca atatttgcga gccgaaacgt ttggctttac cctcgccgat gcccttcacc
     1261 tttttcaggg cggtgaggtt ggccggcagt atttccgcaa tgtctatcag cgctttgacc
     1321 gacatgacct cgtaggcctc catatccaga atttccatca tttcgtcgcg ccattgtttg
     1381 aggcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaccgg caaatccggc
     1441 cgacatgggc gataccggct tgaagtccat cgaagcgtcg gctttggccc gcaggtatgt
     1501 gcgcgcagag aacgagttga ggcaggcgtc gaggcaggat ttttttacaa acaactcgcg
     1561 ttgaaggttt tccatcgcct catcggccac ttttttcacc gctttattgt cggtgatgac
     1621 gggcattttt ttgacggttt cgagcagccc ggtgcctatt ttttccaaaa accagacaca
     1681 ggctttgccc acgcgctctt gcagggcggc gttgtcttcg ggcatttcgg gttgtgcgaa
     1741 atagccgcgc atctgccccc taaacttggc cgctaccgga aacaggtcgg tctcggcggc
     1801 catgtacaac tgctggaagg gctgcacgag gtctttggcg agcgagcctt cgtgcgagag
     1861 gaaagtgcgg ttggcgtgct ccatcttgct tttgatgcca ctgaggtcga acagttccat
     1921 gagcaggctg gcctgatatt cgcgcttcga ctgacgcagg tgttcttcat ctggggcgtt
     1981 tttttcgctc tcagcactga agttgcgcac cgtcgaatct gtttttacgc tcgaataggc
     2041 gatgggggtt cgcagtacga tgccctcgaa actcttgcaa cgactgagcg ccacatacac
     2101 ctgcccatgc gcgaaggcgg cctgcgcgtc tatgatggcc cgctcgaagg tgaggccctg
     2161 gcttttgtgt atggtgatgg cccaggctaa gcgcagcgga aattgcacaa aactgccgat
     2221 gaccgtctcc gacacctctt ttgttttttc gtcgagggcg tatttaacgt tgttccagtc
     2281 ggtggtcgtt acggtgatag gctcttcatc atcaggacat tgcacgatga tttcgtcttt
     2341 gccgatgcgg gtgatgcgcc cgattttgcc gttgtaaaag cgtttttcgc gattggggtc
     2401 gttttttaca aacatgacct gcgcgcccgt tttcagttcc agcacttcgt cggtgggata
     2461 cgcatgttcc gggaactcgc cggtgatttc agccttgaat cggtggagtt cgcccttgat
     2521 gccggctaat ttttcggcgt tgatggcctg tgcggaggcg ttgtgcgagg tgagggtgat
     2581 gtaagcctgg ccttcgggcg cctcaaagcc gggctggtag cggctgttga gttggtcgag
     2641 gacttcggcg gtgagttggt tgtcgcgcac tttattgagc agcgagatnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnngttt gagttcgatg gccaccgttt cggtgagggc
     2761 gagggcgcgg ctgctgaaga agtagggcgt ttcgtagtag gggcgcatca tttcccactc
     2821 gtcttctttg acgacgggcg ggagctggtg caagtcgccg atcatgagca attgcacgcc
     2881 tccgaagggc aggctgcggt ctttgtagcg gcgcagtacg tcgtccacgg cgtccaagag
     2941 gtcggcgcgc accatgctga tttcgtcaat gacgagcaga tcaagacttt ggatgaggcg
     3001 aattttttcg cgggtaaagc gccgttgtcg ggaggcgtcg cggctgtgtt cgggcagaaa
     3061 gaggccgaag ggcaattgga acagcgagtg cagggtcatg ccgcccgcgt tgatggccgc
     3121 cacgccggtg ggcgctgcaa cggccatgcg tttgatggaa tcttttttga ggttttgcaa
     3181 aaaagtagtt tttcctgtgc cggccttgcc ggtgaggaag atgtttttat tggtgctgcc
     3241 gacatagtcg aaagcaagct cgagttgtgg gttggactgg tagttcattt ttgaaaaatt
     3301 agtgagtgaa tcatcttctc gattagtgaa aggcgcgacc atcgttgtat ttgcagggca
     3361 agctacccct tcactatccg ggagaagtgc aaaaataagc ggcggccggc aggtgtgcaa
     3421 gggtggcggg gaaaaatgtt gtaacacttg ccatgcccaa acagcagcgc gccgacaaaa
     3481 cataatttat caaaaaccgg cttgaggttt attcctaatt ttgagggcga accaaaaaca
     3541 caccctcaac atgatgaaaa aagctcttct gctcttcgcg actgcgctat ggtgcagttt
     3601 ctccgcatcc gcccagcagg cgggtactct ggatgtttcg ttcggccaac agggcattgt
     3661 ggaaacgggg tttggggcgg gattgcaggc tatggctatg gaaatccttg cagatggaaa
     3721 gattatggtg gtaggttcgc acgctgaaaa cgatgtcagg agcctgattg tgttgcaata
     3781 tttaccggat ggtgcgccgg atatcctttt tggcaatgca ggaaaaagaa tcgttgcgct
     3841 gcctccgttg gggtatttga tgtttgtaaa aataaatcct gccgacggta gcctgctcct
     3901 cggaagcgga ggcgtcaata gtaatttggc gattacccgc tattcgcctg ccggggaatg
     3961 ggatactttc tttggaaatg gaggcattgt aacagagccg ttcaactacg tcaacaccta
     4021 cgctgatgcc ttatttttgc cagacggaaa aatattagtc ggcgcctatg gttacgcatt
     4081 ttcgccctct cacttaaaag gatatctcat acgattcaac gccgacggaa cattagacac
     4141 atcttttggt gaggagggtt acgcccccat atttatgccg ggcaaccaga ttttgcaggt
     4201 tttggccttg caggcagatg ggagaatact ggccgcaggg catacacatc ccggcacaac
     4261 ttcttcattg ccctggagaa cttttgttgc gcgcttgcat ccggacggca acatagatga
     4321 agattttgga acgggtggaa gcatcttgct tggcattggt gtgccggctt caatcttact
     4381 tcggcccgac ggaaaaatca ttgtcggagg aggacttgaa tcattggata atccgggcct
     4441 gttgtacata gagcaactgc tcgcaaatgg cattcccgat atttcattcg gtacttcggg
     4501 acgagtcagt ctgacaacac aaaggccaat taaagcaagg ccgaaattag aacgcactgt
     4561 cagcggccaa ctggttttgt acacaattct acaataccaa ttggggcttt ggcggttcca
     4621 tgacaacggc agccccgaca cgagtttcgg caatcagggg cttctgacag ccgattttga
     4681 caacggccaa atgagcagca atatcgtgat attgcatcaa caggatcgcc tgctgataac
     4741 cggcaccctt catcgagacg acatcctgtt ggggcgtctt cttcacaacg gcagtttaga
     4801 taccaacttc ggtgaagggg gattttccat cactgatttg ggaagccacc gaatttatgc
     4861 cgggccaatt gcggcctttc cggatggtcg catccagaca tctgcgttgg tgggaaatcg
     4921 tttcgtctcc atgcgctact tacccaatgg caacctcgac cacgcctatg ggcaaagcgg
     4981 gttagcctct gttcgtttgc cccatcatga ctatgtttac gacatttcat tttcgattga
     5041 cgcacaaggt ggtatcgcgg cattaattag tatagaagag gaaaacaaga gtatttacat
     5101 cgcgcggctt cgacccgacg gcactcctga acaaggcttc ggcaccgatg ggcttttgcg
     5161 ggtagttttt ggcttgggag attcttatgg cggctcggtc tctatattgg agaatggaaa
     5221 aattcttatt gccggcactt ttgccaatac aaatggaatt cattttttcg ccatgtccca
     5281 gtttctcccc gacggccagg cagatcaatc attcggaaat ggaggcgtgg ccatatctca
     5341 gaccgccaac aatgcctttt gcagtaatat ggccgtcctt ccggatggta aaattcttct
     5401 gacaggaaat caggaacctg tgccttccag attgatcagc gcccgatatt tctccaatgg
     5461 ttttattgat gcttcttttg gtagctttgg ccgcgttgaa acggcaattg gtcagcagtt
     5521 ggtctcctcc caaaatattt cattgcaatc tgacggaaag attctgattg ccggttctgt
     5581 cgaaggcaaa gcaatactgg tgcgatattt gccggatggc actctcgatt ccgaatttgg
     5641 caatgccggt gtctttatct ctgataacct gacaggttgg aagactcaag taagggttca
     5701 gttagatgga accttatttc tggttggagg gcaacgtacg aacaacattt atacactcac
     5761 ggtaatgcaa ctaaatgcgc agggaatgcc ggaacccaac tttggaacag acggctttgc
     5821 cgttttaccg accgcacggg acatgtatct aaacggctca gaactgctat cagaccataa
     5881 actcgttttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnncctgc cgatgaaatg ctgtacgtcg aatatacgct gccccgtgcc gggcgcgtgc
     6061 atatccgcct gttcgatgtt tacggacgcc cgctgcaaac gctgctccag cccgatcaac
     6121 gcgcagaagg gcctcagcac gagcagtttc gcctgcccga tgcactgccg acgggtgtct
     6181 attggttgag tctggaggcc gaccagaaaa gtgcggccgt gaaagtagta aggcggtaga
     6241 aagtgaggtc tcccggcaac gacacctctc agcccgcaca catgagggga taccctctct
     6301 aagggcagat gtattaaaaa tgtaatttgc agaaagcgct caaaatgcag gactactgtt
     6361 ttctgtcaat gttcattatt ttcccagcgt cgaacatgct tttcaagtcg cttttaaaca
     6421 atggtatagg agtatcaaca aacgctgcat cgcctttgcc tttcaatgta ttcaacccgg
     6481 taactttgtt ggattcatat tcgttcatca aaataaaaac cgtgtctcca gctacgcgat
     6541 caattttgta caaggtataa tgctcatagt cgttcctgat ttcatatatg tcgccttttt
     6601 taggggcgag aatcagttgt gcattttttt tatcgttctg cgcattactt atggatacca
     6661 aaattatcaa cagagtcaac actgcgaggc cggaaaacgt ccatcggggt gtcttactat
     6721 ccgatattaa tgtctcgtag gtactcaaca agctacttga aaattctttc ttctcaagta
     6781 cttgtttaca atgagcacat tgggttacac ctgtctttcc aataggaaaa aacggtatcc
     6841 aaaatacatg agcatatttt tgaaaaacag ccatttgaac actgttctct gccccgcaat
     6901 ttgtacactt gtcgctgagg cttttagatg caatctgtgt tgctttgctt ccgtaaatta
     6961 gcatatagaa gtgattttag ttactttcca atttgcaagg cagtcagcat ttttcattgc
     7021 ctgccttcca cggctnnnnn nnnnnnnnnn nnnaaaatta caattgttac aaataaaacg
     7081 atcacaaatc gcacgcccct accctccaaa caccctgcaa gtgagcagcg taaagtcatc
     7141 ggggtagctt tggttgcctt taaaacgctc aatttgttcc agcagcagac ggttgaactc
     7201 catgggcggc agcaagtagt tggcgcgggc gaagtggtgc agcatggcct catccagaaa
     7261 ctggccggaa ggatcgcgga ggtcggtcag gccatcggtg aaagccatga tgacggcgtt
     7321 gccctccacc gtgaggcggc cgatttctac ctttggcagc gcggtgaagg agccgagaat
     7381 ggtgcagcct ttgtctaaga aacgaaactg gtcgtcggaa acaagcagcg gcggattgtg
     7441 gcctgcattg acgtattgca gactgcgcgt ggcggggtcg tattcggcga tgaagaacgt
     7501 gatgaagcgg tcgcctccgg tgatgcgatt gacggtggta ttgaggtcgc gcacgaagtc
     7561 gtccagagat tcgcgtttgg caatgagggt gtggaaattg gcctgaaaat tggccatgag
     7621 cagggccgca gccacgcctt tgccgctgat gtcgcccacg cagaagacga gcttgccgtc
     7681 gtcgaactcc atgtagtcga agtagtcgcc gcccacgccg agttgcgggc gatacacccc
     7741 ggccagatcg taatggtgat tggtgggcag tgatttgggg atgagcatgc gctgcatttc
     7801 gccggctaat tccatctcgt gtttgaggcg ctcctgctcc aattgccttt tgaaaagcct
     7861 tttattttca atggctacgg caatgacatt ggtgatggcg gtgatgaatt gcaccttgcc
     7921 gtacatgtcg tcgtcttctt taaacccgcc gatgaagaca tacgcgatgg gcagttcttt
     7981 gtgctgcacc ggaatgacga cgtcgaactc gcggatgagc ggattgtcat tgtcctctac
     8041 gtttttgagg cgggtgtagc ggggcagcag cgggcggata tcggttttca gcagttcgtc
     8101 ggagatgccg atggctccgg cacagcgcca gtcttcgtcg tcttcatgca catacagggc
     8161 catttttttg acgccgagtt cccatcccag aaacgatttg tacatgtcgt acagcccgtc
     8221 tgccgacacg ttgttgttga tggcctgggt gatgccgagc agccggttga tctgcaattg
     8281 cttgagattc aactcgcgct ccagcgtttc caagccggag agtccttttt ttatttctga
     8341 aaaaccggac atcccagtgt tttttaaaaa aaacggttat tttctgtgag ctatgagcct
     8401 cgagctgcga gctacgagat gctatcccca ttgacgaaat aaaggtaaat caattccggg
     8461 ataccggggt tatttttcta tttcctattt tcgctgccgg attgcatact tcggcgtgca
     8521 agccacgcaa agaggaaact atctaatgac tgaaaacaaa cttccttatg aaaaactaca
     8581 cgttactcgc cacccttttg tggctctttt tttctgtgca aactatggcg cagcccgtgc
     8641 cgcccaaacg cgaatttcga ggggtgtggg tggccactgt ggccaatatt gactacccca
     8701 aagcgcccga ccaacgctcg gtagccctca aagagcagtg gattcaattg gtggaaatgt
     8761 acaaaaacat gggcctcaat gccgtcatcg tgcaggtgcg gcccgccggc gatgccattt
     8821 atcctaccga aatggcgccc tggtcgaagt acctcacagg caggcagggc ttgccgccgg
     8881 ccgataccac tttcgatccg ctggccttta tgattgagga ggcacaccgc aacggcatgg
     8941 aattccacgc gtggttcaac ccctaccgcg ccactatgga tttggacacg ctgagccttt
     9001 cttccatgca ccttttcaac aaacgccgcg actggatgct gcgctacggc aaccgcttcc
     9061 tgctcaaccc ggcgctgccc gaagtgcgtg aacacatcgc gcaggtggtg ggcgaagtgg
     9121 tgcaaaaata tgacgtggac gctgtgcatt tcgacgacta tttctacccg tacaaagtgg
     9181 ccgaagaggt cttccccgac agcctggaat atttggtact gggcaagtcg tttgccaata
     9241 tagacgactg gcgacgcagc aacgtggacg cgctcgttca gttggtatct gaaaaaatac
     9301 gcgataccaa accacacgta cagttcggaa tcagcccctt cggcgtgtgg cgcaaccgag
     9361 acaaagaccc cgtcatgggt tccgataccc gcgccggact caccagctac gacgacctct
     9421 acgccgacgt gctgaaatgg agccgtctgg gctggattga ctatgtggcg ccgcagttgt
     9481 actggcacat tggtttcacg ccggcagatc attacacgct gctcaaatgg tgggagcgca
     9541 atgcctacgg gcgcttgctg cacatcgggc atggcgctta caaagtggcc aataatcagg
     9601 aagtagcctg gtatgaccct gctgaaatgt cgcggcaaat tgatctcaac cggcgcaacc
     9661 tcaccgcatc gggcagtatt tattttagtt ccaagtcatt ggtcaacaat cctttgggcg
     9721 ttcgggactc catatcgcac tactacgccc ggccttcgct gctgccaatt ccgcctgaca
     9781 aggcgcagaa ggctttcgag gcgccgcaac tgcttcaggt caaaagcaaa aacatacagc
     9841 cgctcctgcg ctggcggccc aatcgcttag atcgcaagaa tccgccgttg tactacgtcg
     9901 tatatcgttt tgaaggcaat actttcggcg attttgaaaa tgcgcgctac atcatgacca
     9961 ttacgccgcg tggtcttact caaaagactt tcgagtttca cgacgccacc gtactccccg
    10021 acaaaactta cacctacacg gtcactgctt tcaaccgcac acatgtggag agcaaagccg
    10081 cgccgggccg gacggtgtac atagggcagt ttaaggtgca acggaaatga tggggcagtc
    10141 tgcagtaggc ggttttcagg ggtgaggggt gagtgtgtga ggggtgagca acccgaaacc
    10201 ttgaacccgg aaccttgaac ctacttgcgg cttcacaaaa atcaactgaa taatttcaat
    10261 aagtaatgtt tctcgaacca catttcaaag ggcaacgcag cggctggata gaggtgatct
    10321 gcggctccat gttttcgggc aaaaccgaag agctgattcg ccggctcaaa cgggccaaaa
    10381 ttgccaatca gcacaccgaa attttcaagc cccgcattga cacccgctac gacgacaccc
    10441 gcgtggtttc gcacgacgcc aattcggtac tctctatgcc ggtggatcac tcctccaagc
    10501 tcctgcaatt ggccgatggc ttcgaggtgg taggtattga cgaggcgcaa tttttcgaca
    10561 tgggcctcac cgaagtatgc caggaattgg ccctgcgcgg cattcgcgtc attgtggccg
    10621 gtttggacat ggactaccgg ggcaagcctt tcggccccat gcccaatctg ctggccgtgg
    10681 ccgagtacat caccaaagta cacgccatct gcccccactg cggcaacttg gccacgcatt
    10741 cctaccgctt atcgcaagac aatgccgaca ttgtgctcgg cgaaaaagac ctgtacgaac
    10801 cccgctgccg cacttgttac agcatgggca atattttgaa attgcatacg taaggttctg
    10861 tggcaaatct tccccgaaca caaaatggtg gaagtctatc gcgccccgga aaccgtagag
    10921 ctatgcagcg gcgccaggct ttgcagcgcc gaacccgttg tggagggctt ggtgatgaaa
    10981 gcagaggata tttttcgaga gatatagcac ttgcctgcca gatatagcgg ggcaaacaaa
    11041 aagcctgccg gctccgacgg aaccgacagg ctttttttgc gagtgggggg gggcctgtcg
    11101 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg ttactacaac
    11161 cgctgcaata ccacccgctt cgtcaccgca ggcagtcgct cgcgcgagat tccatctcgt
    11221 gtgacatcac taacttttac aaaatacatc ccattggcat actgcgacaa gctcagatgc
    11281 tccaccgtcg tttgcacctc ctctatctca ctgaactgca ataacttgcc ttctaagctg
    11341 tacagctcca agcgcacgct gcgaccgaca tactgtgtca aatccacatt gagatcgccg
    11401 ctcgtcgggt tcgggtacac gtcgaactca ggggtgagag gagagaggtg aggggtgagg
    11461 gcgtgaggag tcgacggagt gctgaacccg ctgttgctac ccgtaaaact cactcccgca
    11521 aacgtcgccg tcacggtgct gttggccgtg taattagtgg ctatcannnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccatt gctcgccgat tgctggagcg
    11701 ctgccgcccc gttggtcgtt gtgcggaatt cccggcgatg cagactgctc aggttggtca
    11761 gcaactgcgc ctttttcgcg ccggcggcat tgctctcgcg catgacgagg cccgcccagc
    11821 cgagcgcgcc gctaatgctc gtcacttgtg cggtgatgct gccgttgccg cagagcgtac
    11881 gctgcgcgaa gctggtagca tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnncgggcaa gccggagact gtcacttctg ccgtacaggt
    12061 gctcgtattt ccattgatgt cggtggcggt cacggtcaca ggtacgattt ggcctacctg
    12121 tgcggaagag atggtactgg gcgaaaggcc gatgctttgt acgccgcagt tatcggaagc
    12181 actcgccaga gcgttggact ggagggaaat gctctcctcg ccgttgaaat tcagggtctg
    12241 attgaagcat tgcaccgtcg gagcggtgtt gtccaccttc gttaccgtga aggtgcaggt
    12301 gttggtcagg ccgttgatgt c
//
LOCUS       DAORLF010000087        12229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2773_length_12229_cov_1.762855, whole genome shotgun sequence.
ACCESSION   DAORLF010000087 DAORLF010000000
VERSION     DAORLF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_12229_cov_1.762855"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>271
                     /locus_tag="PK198_09105"
     CDS             <1..>271
                     /locus_tag="PK198_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38936.1"
                     /translation="ETPRSSRIPYFSLISEEALQENVLLRVALKINEQLIQDKPSAIV
                     FHEIHALIQCNENKIAPQVLQNFYAYLRNLCAFLIQMEDDSFLSVL"
     assembly_gap    272..338
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <339..848
                     /locus_tag="PK198_09110"
     CDS             <339..848
                     /locus_tag="PK198_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38937.1"
                     /translation="SPSAYLSIAKTAIKVGNTGWALSFVEDYKDLLFDEDEKEAYYRL
                     NKAECLFSMGQFEEALDVLPAIFSDALYLTHCRRLELKLLYKLNSDLLSYKVDAYKMF
                     LSRASKKLLSDMRREVEGNFINLLVQLINCPPGNKAKAAKIIQRIHEKRVVADKEWLM
                     AQAEDIMAY"
     gene            1064..>1412
                     /locus_tag="PK198_09115"
     CDS             1064..>1412
                     /locus_tag="PK198_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38938.1"
                     /translation="MKTVTFFITQIGRQVRFLLAVLSMVCLGSFAAQGQVVIDEGFET
                     QCGNPPFAFFEGCFPNWISTHGSPDNESNFPGVTAAAGQRYMHGYVKHYYGCSSGPSL
                     TTRGEGVALNFSFQ"
     assembly_gap    1413..1440
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1714..1784
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1785..>1995
                     /locus_tag="PK198_09120"
     CDS             <1785..>1995
                     /locus_tag="PK198_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38939.1"
                     /translation="DAAGNHKDRFCYGEDVYIDGSASAGEDRYYIDLWRRPIGSPNNF
                     NWVSGLGWTVNQQVGVINLSDAFANI"
     assembly_gap    1996..2014
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2015..>2980
                     /locus_tag="PK198_09125"
     CDS             <2015..>2980
                     /locus_tag="PK198_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38940.1"
                     /translation="FQPGYEYRVKLAIANPPFCIPWTEVTHEFVLECCDGFFSADFGL
                     RYDGQPGSPAYLQVHSFPTYSSLHVPVTHTWTVLASPNEYGGPYTLVNTMTTTGAGPY
                     TVLNDVQEGIYYTVIHTVETACGKVCYGKQRYGVDGGMLLGEAVVGNRDADCELCGPI
                     DCGLLGRICLAPIVSVRFDQELDSWVLSWDRVTGVDYFVVEINYGCEPGEPSQFVSVS
                     TFKTQYVLGGGYDPHGYTCLTYRVGYRCPGEEYMVWSDWGLLTPHFTSEESDVSSAMR
                     SENTEDTKLYPNPSKGDVNLLFSAPYTGQVQVMDVTGRVVMTQRI"
     assembly_gap    2981..3130
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3201..>3480)
                     /locus_tag="PK198_09130"
     CDS             complement(3201..>3480)
                     /locus_tag="PK198_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38941.1"
                     /translation="IWSAAREVCGAKPPVNEDQISEAEDRNWEILEAARSHIFRERVG
                     VFFENAPQEDLLAFAEDALLDDEDDPLVSKEGREALFVTLKSVVDLLT"
     assembly_gap    3481..3615
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3698..>5100)
                     /locus_tag="PK198_09135"
     CDS             complement(3698..>5100)
                     /locus_tag="PK198_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF38942.1"
                     /translation="RAKALSLKVQIAAPAFPSNSQPAQLLRQWTQLELQLLSTPENIA
                     LVKRLNDVQKAYRESLTGISPGNRNGAGFSVSSVQAQLDDATAMISYYIGAEQYYTFA
                     LTRNDFKALAIPFNYTTPITRSANSQQPDNTPFPTLRQSVAGLFDALRNTPSGNFAPH
                     ASDLYDKLIQPVRQTIAGKKNLVIIPHDILNHLPFEALLTSPVQTADKKTHHKLPYLI
                     KTHTIQYHFTAAAWPMPGRSSENALAYTAWAPVFDSPETQSALADAMRRSELPLPHLS
                     VYGVPDIPTLSATAAEVQALAQIWSRLPGGARTWLRQDATESSARDMAGQTQIAHFAT
                     YSIAHPLSPRWAGLILTPAGNDDGILHAAEIQTLHWTGALCVLNISYDLTGQSPYALL
                     PLTYAFSQAGAGGVVSALWTGHDQVRIRMLTDFFSRRSAGLSTADALSRAKLALIKDK
                     TTADPSLWSGLIYFGN"
     assembly_gap    5101..5179
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5180..6313)
                     /locus_tag="PK198_09140"
     CDS             complement(<5180..6313)
                     /locus_tag="PK198_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38943.1"
                     /translation="MSQAKFILSLRSLRFLIFLWGTLGHFYAGAQSLREAEALSKSGL
                     FLESIEKYEQFIAQNPRRTYDHSVAWFGIGYNYLQLRDYAAALDANQRSKTIREQLPV
                     DDVAENYMRFGDIYLHIGDYEKALSNLRQAKSLPVVEPYMFAQVEGLMADAYLALGNY
                     REAEAHYQLALESFAVESGEFSPDVVTVLLQLGKMYQIQGRKPDAKETFLRALRVEET
                     LSEHAARLGLLFNGLGESALSESRSPAARSYFLRARAAIGQEGSAHYDVAASIALNLS
                     KAALADGDRGLAAAEVMEAQRILFPGFLATDFLQNPDTTLPCLHRVLAAEVCLQKANC
                     LQGAPAALPSQGTAQYYSEAFRLLEAEALTFPSDERRRHIMDFL"
     gene            complement(6456..6851)
                     /locus_tag="PK198_09145"
     CDS             complement(6456..6851)
                     /locus_tag="PK198_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38944.1"
                     /translation="MKLFNGLAVCLALSVALLGFGCDKEKNEVTINIVKPVANQAVAN
                     KAAVEILVQFVASEENEEVEVHIYQHGDSSNPVFEWHEHEHDPLITLAETIDLSSFPS
                     GTKFHLEAEACLDHKCKEKVSKEIEFTIP"
     gene            complement(7317..7556)
                     /locus_tag="PK198_09150"
     CDS             complement(7317..7556)
                     /locus_tag="PK198_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38945.1"
                     /translation="MKIYESDKTFYGSRLTRVLSCTLYFSVAEGNPAEAAASAEAAAC
                     TVLPQQEKVFVFASRLFSNSNQTVPRINFSVVVRK"
     gene            7710..8438
                     /locus_tag="PK198_09155"
     CDS             7710..8438
                     /locus_tag="PK198_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38946.1"
                     /translation="MKNALMCAVGLLAFALAAVVSPVYGQAGPSVEKGSLFPEKGIQL
                     IYQATSPGYFDSYSGSMSWVSDENGGQITHAVWREETPTPFNFTFNYSLVKDADRGYL
                     MDMKGIMSPFHFMINESVKVSYEGDRLFFPNSFQSGQTLPDAKGAFNLEIGKGIPDLR
                     YEVSVSDRKVATGEPMLIEGQSYETFIHTCRVATSKVLGGNRLEESDVEWMKDWFVPG
                     LGIVKRETGKFTATVQSIKQNSDK"
     gene            8836..>10776
                     /locus_tag="PK198_09160"
     CDS             8836..>10776
                     /locus_tag="PK198_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38947.1"
                     /translation="MNPGFENGLWEWGPWGTVAATATAYSGVGAAQLTGSNSGVSQIF
                     FGSAGTTYTATVRAQAQNTNWGYIQIEFFNSSWQIIGTSPQGHFGTPSTYTQFSTTGT
                     APSGTAYYQVVIGSSSNSGGYVRVDDVCVTTSGSSSCAPCTGNLVTNGHFNVNTTGWN
                     SWNGNFAKSTSWPQCSSAGNGEIQHTTGWAGFSQDISGIAPGASIALTFWAGVHDPSY
                     TATFGVQFYNASGGALGQLVENIDRVLGGTPSMQFYTINTTVPAGTATVKLVGSLNGG
                     WLKVDEICLTSSTSSCNPDNPNAPPYCAPTPNCTNGTGTLLWSQSIDSNTGNPSTVRM
                     YCGSTTSYTIPGPYPAAFNSAVTVNVTDAVGWDGYSSRGSVSQPNERFRIVFKKNGVT
                     VASTPYSGDVPDNVIQGYWRGALGGSITLPNGADAIIIEHWSVANDCNSPNSLVPSSI
                     CISYANCNLSLSSHPSNQTICQGGTASFTVAATGASSPTYQWQYYNGSSWVNVANGTP
                     AGATYTGATAATLQVAGVSATGAYQYRAAVSASGCTTVNSNPATLTVTPDPTISITPS
                     SSTVCVGGTVTLTATPGNGTGTCTIQWQSGTSSTGPWTNISGATSTTYSVPTSTAGTR
                     YYRATYTCTGSGCDAANSGASTV"
     assembly_gap    10777..10866
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11253..>11955
                     /locus_tag="PK198_09165"
     CDS             11253..>11955
                     /locus_tag="PK198_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38948.1"
                     /translation="MQMQAVNKANSSLVFNITVILRNRTYSPPAGSPKENTQCVGDIN
                     NSNWYYYTAMEGVLEGANALAGGIVTFTRMGESFQIGTGANLNNGSVFGASGWLNQVV
                     KKQPSSGTLFTANPSHGDFNVNLSGATLPNATAPCLNICSGESVTLTANGLAGQSPYT
                     FQWSTGATSSTITVSPTSTTTYSLTVTDANGCSNTDQVQVNVNSVTPGTVGSNQTICV
                     GGDPAAFTVTQAASGG"
     assembly_gap    11956..12050
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2898 a         2684 c         2937 g         2976 t
ORIGIN      
        1 ttgagacccc aaggtcatcc aggatacctt actttagcct gatttccgaa gaagccttac
       61 aagaaaatgt attgctcagg gtagctttga aaataaacga gcagcttatt caggataagc
      121 cctcagccat agtattccat gaaattcatg cgctgattca gtgtaatgaa aataaaattg
      181 ctcctcaggt acttcagaat ttttatgcct acctcagaaa cttatgtgct tttctgatac
      241 agatggaaga tgattctttt ttgtctgtac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gccgagcgct tacctcagca
      361 ttgcaaaaac agcgataaag gtaggcaata cagggtgggc tttgagtttt gtggaggatt
      421 acaaagattt gctgtttgat gaggatgaaa aagaagcata ttaccgcttg aataaagccg
      481 aatgtttgtt ttctatgggg cagttcgagg aggcattgga tgtgttgccc gcgatcttca
      541 gcgacgccct ttatcttaca cattgccggc ggctggagct caagttgctt tataaactca
      601 attccgatct tctctcatac aaagtagatg cctataagat gtttctcagc agagcatcga
      661 aaaaactact atctgatatg agaagagagg tggaaggcaa ctttatcaat cttttggtac
      721 aacttatcaa ttgccccccc ggcaacaaag ctaaggctgc aaaaatcata cagcgaatac
      781 atgaaaaaag agtggtggcc gataaagagt ggttgatggc acaagcggaa gatataatgg
      841 cttattgacc agtttataga aaaaaaagta tttttcgtta cggatatctg agacttaagt
      901 aattgtattt caatccttta aggtgatatt gtactcggaa ttgctcagcg aaccgccaga
      961 tgccccggtt tttattgaaa taagtgttat tgtaaccagc gaatctccga ccacaaattt
     1021 gagcccgaaa tcattattcg ttcactcaat aaatttgatg gtcatgaaga cagttacatt
     1081 ttttattaca caaatcggcc ggcaggtgcg cttcctttta gctgtactga gtatggtttg
     1141 tctcggctct tttgcggcgc aagggcaggt ggttattgat gaggggtttg aaacgcaatg
     1201 cgggaatcca ccgtttgcgt tttttgaagg atgtttccca aactggattt ctacacacgg
     1261 cagcccggac aatgaatcga atttcccagg ggtgacggct gctgccggac aaaggtacat
     1321 gcacggctat gtaaagcatt attacggatg ttcaagcggt ccaagcctga ctacacgcgg
     1381 agagggcgtt gcgctgaatt tttcctttca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 tgagatacgc ccatcaagcg agaagaagcg ccggaacata tactggccgg tggacgctta
     1501 caaacggcct tcccaatcag aatggcttat tttgctcacc tgcggaagta gttcctgcgg
     1561 taccacctgg ttctcaggtt gttggaagcg cttctttaag cccaaactgg gtgttttata
     1621 ctcatgattt tacccccaat gcaaattttc agcaactttg gttcagggca acccttatac
     1681 acccaacaat caatgctgaa gaaaatgggg actnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatgct gccggcaacc
     1801 acaaagaccg gttctgttat ggcgaagatg tgtatataga tggctcagcc tcagcgggcg
     1861 aagaccgcta ctacattgat ttgtggaggc gccctattgg aagcccaaac aattttaatt
     1921 gggtttcggg cttgggctgg acggtaaacc aacaggtggg agttatcaat ttatcggacg
     1981 cttttgccaa tatcgnnnnn nnnnnnnnnn nnnncttcca gcctgggtac gagtacaggg
     2041 tcaagctggc cattgccaat cctccatttt gcattccatg gacggaggtg acgcatgagt
     2101 ttgttttaga atgttgtgac ggattctttt ctgccgattt cggcttgcgt tacgatggac
     2161 agcccggcag cccggcatat ttacaagtac atagtttccc aacctattca tcgcttcacg
     2221 ttccggttac gcatacctgg acggttttgg caagccccaa tgaatacgga gggccatata
     2281 ctctggtcaa tacgatgaca acgacaggtg cagggccata caccgttctt aacgatgtgc
     2341 aggagggaat atattatacg gtaatccata cggtagaaac tgcttgtgga aaagtgtgtt
     2401 atgggaaaca gcgttacggt gttgatggag gaatgctgct tggagaagca gtggtgggga
     2461 atcgtgatgc agactgtgag ttatgcggcc cgatagactg tggtttatta ggcagaattt
     2521 gtcttgctcc cattgtaagt gtgaggtttg atcaggaatt ggattcatgg gttttgtcat
     2581 gggatcgagt gactggggta gattattttg tggtagagat caactatgga tgcgagccag
     2641 gagaaccctc tcagtttgtg tctgtcagta ctttcaaaac tcaatatgta cttggcggtg
     2701 gctatgaccc gcacggctat acctgcctta catacagggt agggtacaga tgccccggag
     2761 aagagtacat ggtatggtcg gattgggggt tgttgacgcc tcattttact tcggaagaat
     2821 cggacgtatc atcagcgatg aggtctgaaa atacagaaga cacgaagttg tacccgaacc
     2881 catctaaagg ggatgtgaat ctcctgttta gtgcgccata taccggccaa gtgcaggtga
     2941 tggatgtgac aggcagagtt gttatgacac aacgcattga nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn aggccggcag aagaaattct gtcggctttt tgatttttat atctccttcc
     3181 aacgacaacg ttaatattca ttacgtcaac aaatccacca cagacttgag cgtgacgaac
     3241 aacgcctcgc gtccttcttt gcttacaagc gggtcatctt cgtcgtcgag gagggcatct
     3301 tcggcaaagg cgagtaaatc ttcctgtggg gcgttctcga aaaacacgcc tacgcgttcg
     3361 cggaaaatgt gtgaacgagc ggcctccagg atttcccagt tgcggtcttc tgcttcgctg
     3421 atttggtctt cgttgacggg aggtttggcg ccgcacactt ctctggcggc actccagatg
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnncataa gcgccgggcg tgttttcgag gcggtctatg tggtggtcaa
     3661 tgacggtttc ggatacaaaa gccatattcg tttcagatta attgccaaaa taaatgaggc
     3721 cgctccatag gctggggtcg gcagttgttt tgtctttgat aagcgccagt ttggcccggc
     3781 ttagggcatc ggcggtagag aggccggccg atcgccggga aaagaaatca gtcaacatac
     3841 ggatgcgtac ctgatcgtgc ccggtccata atgccgaaac taccccgccc gcgccggcct
     3901 gggagaaggc atacgttaag gggagcaacg cataaggact ttgccccgtc aggtcatagc
     3961 tgatattgag cacacagagg gcgcccgtcc aatggagggt ttgaatttct gccgcgtgca
     4021 agattccatc atcatttccg gcgggtgtga ggatgagtcc ggcccaacga ggcgataagg
     4081 gatgagcaat gctatatgta gcaaaatggg cgatttgggt ctgcccggcc atatctcgcg
     4141 cggaagattc agtagcatct tgccgaagcc atgttcgggc gccgccgggg agtcggctcc
     4201 atatctgagc aagcgcttgt acctccgctg ctgttgccga gagcgtgggg atgtctggta
     4261 cgccatatac ggaaagatgg ggaagaggaa gctctgaccg acgcatagca tctgcgagcg
     4321 ccgactgggt ttcggggctg tcgaaaaccg gcgcccaagc ggtatatgcc aatgcgtttt
     4381 cggaactgcg gcccggcatt ggccaggcag cggcggtgaa atgatattgt atggtgtgtg
     4441 ttttgatgag gtaggggagt ttgtgatgtg tttttttatc ggcagtctgg acgggcgaag
     4501 taagaagtgc ctcaaaaggc agatgattca gtatatcgtg cggaatgatg acgagatttt
     4561 ttttgcctgc gattgtctgg cgtaccggct gaatcaattt gtcgtacaag tcggaggcgt
     4621 gtggcgcgaa attgccggag ggggtgtttc ggagcgcgtc gaagaggccg gcgacagatt
     4681 gccggagggt ggggaagggt gtattgtccg gctgctgtga attggccgac cgagtaatgg
     4741 gagtggtgta attgaaaggt atggccagtg ctttaaaatc gtttcgcgtc agcgcaaaag
     4801 tataatactg ctctgctccg atgtaataag aaatcattgc ggtggcgtcg tcaagttggg
     4861 cctgaacgga ggaaacagaa aagcccgcac cattacggtt gcctggggat atgccggtaa
     4921 ggctttcccg atatgctttt tggacatcgt tgaggcgttt taccaaagct atattctccg
     4981 gggtgctcag tagttgaagt tctaattgtg tccactgccg aagcaattgc gcaggttggc
     5041 tgttggaggg aaatgcggga gcggcgattt gtaccttgag cgacaaggct ttggcgcgtt
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnc agaaaatcca taatgtgccg gcgtcgttcg tcggaaggga
     5221 atgtcaatgc ctccgcttcg agcaggcgga atgcctccga atagtactgt gctgtgcctt
     5281 gcgaaggtaa agcggcagga gcgccctgga ggcaattcgc tttttgcagg cagacctcgg
     5341 ccgcgagtac gcggtgcagg caaggcagcg tagtatctgg attttgaagg aagtcggtgg
     5401 ccaaaaatcc agggaaaagt atccgttggg cttccatcac ttccgcagca gccagtcccc
     5461 ggtctccatc ggccaaagct gctttgctga ggttgagcgc aatgctggcc gctacatcat
     5521 aatgagcgct tccttcctgc ccgatggcgg cacgtgcgcg caggaagtag ctgcgggcgg
     5581 cgggcgacct ggattcggac agggcagatt ctcccaaacc attaaagagc aagcctaagc
     5641 gcgcggcatg ttccgacagg gtttcttcga cgcgcagggc gcgaaggaag gtttctttag
     5701 cgtcaggttt ccgcccctgt atttgataca ttttgccaag ctgaagaagt acggtcacta
     5761 catccgggct gaattcgccg ctttccacgg caaagctttc gagggctaat tggtaatgcg
     5821 cctccgcttc ccggtaattt cccaaggcca aatacgcatc agccatcagc ccttccactt
     5881 gtgcaaacat gtaaggctct accaccggaa gcgatttggc ctggcgtaaa ttgctcaagg
     5941 ctttttcgta atcgccaata tgaagataga tgtcgccaaa gcgcatataa ttttcagcca
     6001 cgtcatccac gggtaattgc tcccggatcg ttttagaacg ctggttggcg tcgagcgcag
     6061 cggcgtagtc cctcagttgc aggtagttgt acccaatccc aaaccaggct acggaatgat
     6121 cgtaggtgcg ccgggggttt tgggcgatga actgctcgta cttttcaata gattctaaaa
     6181 aaaggccgct tttactgagc gcttcggctt cccgcaaaga ttgggctccg gcgtaaaaat
     6241 gccccaaagt tccccacaaa aaaatgagga aacgcaggga tcgcagtgac agaataaatt
     6301 ttgcctggct caataggtct gatgttgttg gcatgtagaa atgatatgct gatgcgcgca
     6361 caaagatacc cgattgtata taatgggcac aaaaaaggcc ggcagaattg cttcgccggc
     6421 cccgaattgc gattacttga actttacatt ctttatcagg ggatggtgaa ctcaatttct
     6481 ttgctcacct tttccttgca tttgtgatcg agacaggctt ccgcttcgag gtggaatttg
     6541 gtacccgacg ggaacgaaga caagtcaatg gtttccgcca aggtgatgag tggatcgtgc
     6601 tcatgctcat gccattcaaa caccggattg gaggagtccc catgttggta aatatgcacc
     6661 tctacctctt cattctcctc agaagcaacg aactgtacca gaatttccac agcggctttg
     6721 ttggctactg cctgattggc tacgggcttt acgatgttga tcgtgacctc atttttttct
     6781 ttatcgcagc cgaaacccag tagggcgaca cttaacgcaa ggcatactgc cagaccattg
     6841 aatagtttca tacgaatgat tttttagtga acgtttttgc atcaacgctg caaaaatgag
     6901 atttgttttt ggtatttgca atggtgttgc aaaaaaaaac actgcacctt tcttttatag
     6961 acggggatta acagaatttg atcggattat gtaaagctgc tttggaaaat ggaggcaggc
     7021 atacagctag ccgcagtttc gtcatttttt tttcaagatg tttcgacctt ccaatgtttc
     7081 aattttttta aaaattatta gtgtgatttt ataattttta taattacgtc ttaaatattt
     7141 atattttata tcttttaatt gcattgtatt ttgcattttg ttaaaaatta ttaaaattat
     7201 aatgtaaata tttttataat atcatgtcac aaaaatataa attaagcaat gtgaccgtct
     7261 tatttaggta gtacccggta gtaactttgc gcgtcgaatg tgacatacgt cctcccctac
     7321 ttacgaacaa caacactaaa attgattctc ggcactgttt gatttgagtt agaaaaaagc
     7381 cgggaagcaa aaacaaacac tttttcttgt tgcggcaaca ccgtacaagc ggcagcctct
     7441 gcggaggccg ccgcttccgc cggattccct tcagcaacag aaaagtacaa agtacagctc
     7501 aaaactcttg tcagccttga gccatagaat gttttgtctg attcgtaaat tttcacataa
     7561 cgtttttaca tatcaagtgt cctatgagaa ctttttacac taccggctgc actgtgccgg
     7621 gaaattttct tcccaaaacc gacgcatctc acatgccttg tggacctaat acaggtcatg
     7681 cgcttccatt caaccaacat actgcaaaaa tgaaaaacgc actcatgtgt gccgtcgggc
     7741 tactggcatt tgccttggcg gctgtggttt cgcccgttta tgggcaagcc ggcccttctg
     7801 ttgaaaaagg aagcttattc ccggaaaaag gaattcagtt gatataccag gcgacttcgc
     7861 ccgggtattt cgatagctac tcgggtagta tgtcttgggt gagcgacgaa aacggcggcc
     7921 aaataaccca tgcagtatgg agagaagaaa cgcccacgcc tttcaatttt actttcaact
     7981 attccttggt gaaggacgcc gacaggggct acctcatgga tatgaagggc attatgagtc
     8041 cttttcattt tatgatcaat gagtcagtga aagtctctta tgagggagat cgcttgtttt
     8101 ttccgaatag cttccaatcg ggccaaacgc ttcccgatgc aaaaggcgct tttaatctgg
     8161 agatcggcaa gggtattccc gatttacgat atgaagtcag tgtatcagac aggaaagtgg
     8221 ctacaggaga gccgatgctc atcgaaggac aaagctatga gacattcata catacatgcc
     8281 gggtagcaac ttccaaggtt ctcggcggaa acaggttgga ggaatcagac gtcgaatgga
     8341 tgaaagactg gtttgttccc ggcctcggca ttgtgaaacg ggaaaccggc aagtttacgg
     8401 ccacagttca atctatcaaa cagaactccg acaaataatc atttcgtgaa agcaagtatc
     8461 tcaacatact caaaaaatca acacatgaac ggcacattca cccaaaagat aacacaaagc
     8521 ctggcagtag cagccatcag cctgctcagt ctggcttatt ctacggaggc ctcttcggct
     8581 acctgccttt ggtacacttg caccggcacc agtggcagta acaccattta cctgaattgc
     8641 aatcaatgta cgggtagtac agttaccagc acatgtaata gccctaatgc cgggttaccc
     8701 gaagtggggg atcaatactt ttgggacggc tcgcaatttg tgaatatgac caattcgttc
     8761 aggcagaatt caactggcac gcctacgagc tgttccggta cacaatcttc gacatcctgt
     8821 accagtgcat tcctagtgaa ccccggcttt gagaacgggc tttgggagtg ggggccttgg
     8881 gggacggttg ctgccaccgc tactgcatat agtggtgtag gcgccgcgca attaaccggc
     8941 agcaattccg gggtgtctca gatttttttc ggctcggcag gaacaactta cacggcaacc
     9001 gtcagggcac aggcccaaaa caccaattgg gggtatatcc agattgagtt ttttaattcc
     9061 agttggcaga ttataggtac ttctccccaa ggacatttcg gcactccaag cacctacact
     9121 caattctcaa ctaccggcac ggcgccttca ggtactgcat actatcaggt ggttatcggg
     9181 tcaagttcca actccggcgg ttatgtcaga gtggatgatg tgtgtgtgac gaccagtggt
     9241 agctcatcct gcgcaccctg taccggcaac ttggttacca atggccattt caatgtcaat
     9301 acgactggtt ggaattcatg gaacggcaat tttgccaaaa gcacctcctg gcctcagtgc
     9361 agcagtgccg gaaatggtga aattcagcat acaactggtt gggcagggtt ttcgcaggat
     9421 atttcgggta tagcaccggg ggcctcaatt gccctgacat tttgggccgg ggtacatgat
     9481 ccgtcttata ctgcgacctt cggagtgcaa ttttataacg catcaggggg tgctctgggg
     9541 caattggtgg agaacattga cagagtgctt ggcggtacgc cctccatgca attttatacc
     9601 attaacacca cagtgcctgc gggtactgcg actgtcaaac tggtagggtc attgaatggt
     9661 ggctggttaa aggtagatga aatttgtttg acttcttcca cttcgagctg caatcccgac
     9721 aaccccaacg ctccgccgta ttgcgcaccc actcccaatt gtacgaatgg aaccggcacc
     9781 ctgttgtggt ctcagtctat tgactcgaat acaggcaatc cttcgacggt aagaatgtat
     9841 tgtggcagta ccacatcata taccatacct ggtccatatc ccgctgcatt caatagtgca
     9901 gtaacggtaa atgtaacgga tgcagtagga tgggatgggt atagcagcag aggcagcgta
     9961 tctcaaccca acgaacgttt tcgaatcgtt tttaagaaaa acggagtaac tgttgcctct
    10021 acaccctact ccggagatgt gccggataat gtgattcaag gatattggag aggcgccttg
    10081 ggaggcagca tcacgctgcc gaatggcgcg gatgccatta tcatcgaaca ctggagcgta
    10141 gccaatgatt gcaatagccc caactctttg gtaccgtcca gtatttgtat cagttatgcc
    10201 aattgtaatc tttcattatc ttcccatcct tccaatcaaa ctatttgcca gggcggtacg
    10261 gcttccttta cagtagcagc taccggggca tcttccccca cttatcagtg gcaatattat
    10321 aatggttcct cttgggtaaa cgtagccaac ggtacacccg caggcgctac ttatacaggc
    10381 gccacagcgg ctacattgca agtggcaggt gtctccgcta cgggtgctta tcaatatcgc
    10441 gcagcggtat cggcttcggg gtgtaccacg gtgaactcta acccggcgac gttgacagta
    10501 acgcctgatc ccacgatcag cattacacct tcttcttcaa cggtttgtgt aggcggcacg
    10561 gtgactttga cggctactcc gggcaacggc acgggtacct gcacgataca gtggcagagc
    10621 ggaacgtctt caaccggccc ctggacgaac atcagcggtg caaccagcac gacctacagc
    10681 gttcctacat caacagcagg caccagatat tatcgtgcaa catatacttg tacggggagt
    10741 ggctgtgatg cggcgaattc gggcgcatca accgtannnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnngcac cccttccctt acataccaat ggcagtcaag ctccaacgga tcatcttggt
    10921 ccaatataag cggcgccacc tcggcctctt atacaacgcc cactttaacg gccaccactt
    10981 attatcgagt catcgtaagt gcgggtggtt cagggtgtgg ttctgcaacg tccaacaccg
    11041 ctaccgtaac catttacggc aagccctcgg cagtcatcac ttgccagagt gaccctcttt
    11101 tggtacgaac ggtgtcaaac agtaccaaga cctgcgatga tttggtatcg tatggctatt
    11161 ggtcgggtga tttggtaaac aattggacga gccaacagca ctggaatgta agcaacgcca
    11221 cttttgagga gtatgccaat ggaactgcca aaatgcaaat gcaggcggtc aataaagcca
    11281 attcaagcct tgttttcaat atcacagtca ttcttcgcaa tcgtacttac agcccaccgg
    11341 caggcagtcc caaggaaaat acacagtgtg tcggtgatat caataattcc aattggtatt
    11401 attacacagc aatggaaggt gtgttggaag gcgccaatgc tttggcagga ggtattgtca
    11461 cttttacccg aatgggagaa tccttccaaa taggaaccgg cgctaatttg aacaatggct
    11521 ctgtatttgg cgcttcgggg tggctgaatc aggttgtaaa aaaacagccc agttcaggca
    11581 cgctctttac cgccaatcct tctcatggcg acttcaacgt aaacctgtct ggcgctacat
    11641 tgcctaatgc aacggcacca tgtcttaata tctgctccgg cgaatcggtt acacttacag
    11701 ccaacggcct tgcaggtcaa agtccttaca ccttccagtg gagtacaggt gccacctctt
    11761 ctacaattac tgtatcgcca acgtccacca ctacttactc gcttacggtg accgatgcca
    11821 atggctgctc taataccgat caggtacaag tgaacgtgaa cagcgtcacc ccgggtacag
    11881 tcggctccaa ccaaacgatc tgtgtcggcg gcgatccggc tgcatttacc gttacacaag
    11941 cggccagcgg cggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacttatgac
    12061 gcacctgcgg gcctcacgca gacaacatat tatcgtcgtg tggctactgc aacccttaat
    12121 ggagtggttt gtagtgcaaa cagcaattgc attacagtaa cagtaacgcc cgaccccacg
    12181 atcagcatta caccttcttc ttcaacggtt tgtgtaggcg gcacagtga
//
LOCUS       DAORLF010000088        12208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2782_length_12208_cov_2.029622, whole genome shotgun sequence.
ACCESSION   DAORLF010000088 DAORLF010000000
VERSION     DAORLF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_12208_cov_2.029622"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>433)
                     /locus_tag="PK198_09170"
     CDS             complement(<1..>433)
                     /locus_tag="PK198_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38949.1"
                     /translation="SGAVISPNPTCAGTKTYTWTYTDCAGNPYNWVYTYTITGGTGGP
                     MPPNGASTVACPSGANVPTPPVVNDNCGNPMQISGPVISPDPACAGTKTYTWTYTDCQ
                     NNTYPWTYTYTILGPTVSMPAPGAGTVSCASAAVQPTPPNVA"
     assembly_gap    434..462
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<463..3212)
                     /locus_tag="PK198_09175"
     CDS             complement(<463..3212)
                     /locus_tag="PK198_09175"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38950.1"
                     /translation="MASNSTRPKCAFYILLLTSLWMFGGHIGKLNAQCTGPTLNLPDA
                     SSQPVLNPPNPYCTAINIDPAVTGHPIGLQMMLEHDWQGDLSIRINACGGTLMLLTRP
                     GGGSCTGGCACGSSADIGTVGNPALVSFSDGGGPNPDLGIPITGGNFGLSTDPCGANS
                     MISSFASLASLCGGAPYTIQVCITDHVGANDGIATNIRPMFPGIVCGCNDPAALNYNP
                     AATVNDGTCTYPPPPLTCGSVFYDSGGPSGNYSNNENATTTICPTTPGDIVTVTFTAF
                     NTESSYDKLYVYNGPNASSPQISSGNGSGSGICSTPGGFWGTSIPGPFTSTHPGGCLT
                     FVFCSDGSLTYSGWAANVTCSTPCTPPPAPTASGQTIPPNTSCTLTASGCAGTLRWYN
                     APSGGTQVGTGSPFVTPLLSATTTYYVACDTGSGCASARTPVTVTVQNPPAMPSCGSN
                     TWNVSVYADNTYTTFMGFYNTPGHSISPGDGGNDPLDIEFNVGKDGVPVNGFPSQANG
                     YTGLPLTNEAFSIRAERCGFPCGAYYYINVRAFDDHVRVRVDQTGDGTYEFDYSNGPP
                     RSTTPTDIWNGFLGPNSRVIIEGYNIGPGHPLYVSPNEFFLHVAFYNDQNHPLLTANA
                     GADANFCSPGSVGIGGAPAAAQGVPGYTYSWSPAGTLSNSGIANPTASPATPTTYTLT
                     VTDARGCTATDNVIVTPMLCCAANAGAVAINPNPQCPGEMFTASATGHQTTSGYTQTY
                     ILTNSSGSVLYIIPSGNFTTPVACGTYSVYSYNYLTAGTAITNPATIGQIVCQPTHCC
                     DLSAPLAFTTSATVTMPTDAGSTVACVSAATAPTPPDVMYSCGGLLARSGPVISPDPA
                     CVGVKTYVWTYTDACNSQTYNWTYTYTISAPTVSMPAPGASTVGCASAAVQPTPPNVT
                     DN"
     assembly_gap    3477..3522
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3582..5711
                     /locus_tag="PK198_09180"
     CDS             3582..5711
                     /locus_tag="PK198_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRF38951.1"
                     /translation="MNFDTRNIRNVVLLGHSGSGKTTLAEAMLFEGKAISRRGKVEDQ
                     STVSDYTDLEHERGNSIFGTLLHVLWKDSKINIVDTPGSDDFVGEVISSLKVADTGVV
                     ALNARSGVEVGTELIWEYIEKFKTPALFAINHIDHEKADFDTTLEQARQRFGNHVIPV
                     QYPLNPGAGFNKIVDALRMVMYVFPEGGGKPDKQPIPASEQERAMEMHNALVEAAAEN
                     DEGLMERFFEQGTLSEEELTEGLRIAIAHQQIFPVFCCSALQDMGSGRIMGFINDVCP
                     SPADRPAAELEGGGTLACDSKDSATVFVFKTVSEPRVGNLSYFKVYSGVLHSGDELVN
                     VENRSAERFGQIYLANGKDREGVNALMAGDIGVTVKLKNTHTNNTLNIKGVDRKIDPI
                     HFPTPRIDVGVHQPNKNDMEKLMKALHTIAEEDPTLIIEQSKALKQTLLYGQGQLHLD
                     LIKHRIEMVYGVSMEYVRPRIPYRETITRVANTMYRHKKQTGGAGQFAEVHMRIEPYH
                     EGMPAPTDLSVKNVEVDELPWGGKLVYQWCIVGGTIDARFSNAIKKGIMNKMEEGPLT
                     GSSCQDIRVSIFDGKMHPVDSNDMAFMVASVQAFRQAFAEAAPQLLEPIHDVEILCSD
                     EVMGDVMGDLQTRRAIIMGMDSDGHYQKILARVPLAELYLYSSSLRSFTQGRAKFTRA
                     FAEYAQVPGDIQQRLIEAHKAETEHEA"
     gene            5916..>6488
                     /locus_tag="PK198_09185"
     CDS             5916..>6488
                     /locus_tag="PK198_09185"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF38952.1"
                     /translation="MKAIKIAGILLLALIVLVLVLNALAPKTFEMERSIVIDAPRETV
                     FPYVQYLEQQSRWSPWSRLDPNMKTTLTGIDGTVGAASAWDGNKDAGKGSQTLSLVQP
                     NERVETQLAFIKPFESTAQAWFQLKDAEGNSTEVTWGFRSPMPFPFNVFGLIMGMEKE
                     MDAQYTQGLADLKALVEKEAAAAPALKVMES"
     assembly_gap    6489..6498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6499..6943
                     /locus_tag="PK198_09190"
     CDS             <6499..6943
                     /locus_tag="PK198_09190"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRF38953.1"
                     /translation="AATPRYYVGIRETVAIPQLTERMALHFPKIAQALQAAGIELDGM
                     PSGLYFTWDEAAQTTDFAAAFPVKSKVEIKGFTTVEMPAGTRLSVDYYGPYEKIAPAH
                     EAIDAYIQRNKWQHGAMAVEEYVTDPGMEPNPAKWLTKVTYPVSK"
     gene            7110..9047
                     /locus_tag="PK198_09195"
     CDS             7110..9047
                     /locus_tag="PK198_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF38954.1"
                     /translation="MLRKHLYSFLFLLTGLSAAAQNESVATLKARLESAKDPAKVIQL
                     LGVLADSTQKSDVQALRPLAQQALRLAGGNLSDTLKARTYLIAGKALAQMVSYDSAAM
                     FLDRSLALLDPSRQAAETAEANHWKGFAKANQREFAAASEHYYQAVEIWERIGRRRAL
                     AETYNQLSDMHAMQEDYEKSIHYAQQAIAQFESMDAPELLADALNNLSYVHVLKGDYA
                     GALQYATEALQIWERIAPDGLQVARTSNSRGNAHKFLGNYEEALLDFERCRSIAEKLG
                     FVRGVIAGTANKGHVLLMQKKYAEALPYTLRAIELMKESGDTRNLWENYMHLSNIYAG
                     MGDYPNAHRYQQLHQEEKEREYEQKIAALQGGLAEKYEAGQRAATIAIQKERIEKQRN
                     FQRLLWGVAGLLLLTSVLIYMSLRSRQKANRLLEAANEQLAKKNRENELLLREIHHRV
                     KNNLQTISSLLNLQSAGIEDAAALAAVQESRNRVRSMSLIHQKLYQGEQLASVEMKDY
                     FETMGQAMLQSFGAAGEQIALKVDMPTIDLDVDTAIPIGLIVNELMTNSLKYAFPEHR
                     GGAIEISLRAEDEHTLALRIADNGVGGNSEGGSEWRESSTGFGGKLVKLLAQQLGARM
                     EQSETGGYTTILRFPNPAKAA"
     gene            9051..9803
                     /locus_tag="PK198_09200"
     CDS             9051..9803
                     /locus_tag="PK198_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRF38955.1"
                     /translation="MSAVKILIVEDEMIIAADISMMLESIGYEITGIMPRGEDALKSI
                     ESTPPDLVLMDISLKGVLDGVATSQFIYERHQIPVIFLTANADDATFARAKATRPYAF
                     ISKPFQPADLQRAIELALARLAEEHSGQSLLHVNDKAEDAYILADRIFVRYKGKMTKI
                     LLQDILYAEADRSYCKIYTPQAEYLMSISLGALEEKLRTDNFLRIHRSYIVNLHKIDT
                     ISDDWGYLGIGAQTLSVSRSFKDELVRRFHII"
     gene            complement(9777..10049)
                     /locus_tag="PK198_09205"
     CDS             complement(9777..10049)
                     /locus_tag="PK198_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38956.1"
                     /translation="MQQQAERQAIWVLDVYKKIVIDTSSFVLRRMCGVYLGCFRMVTP
                     IDAIKLQHAVLCSKGEFPNDGFEFPKDGFGVPNEMLRCSNNMKPAH"
     gene            10264..>11112
                     /locus_tag="PK198_09210"
     CDS             10264..>11112
                     /locus_tag="PK198_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF38957.1"
                     /translation="MKKLSATIGEAFESIRARLFHTLLSVLGIVIGVAALVTILSLID
                     GMEKHARRQLSDTTSVKMIQISSVTTKTVQGMNISKDSFPYLNYEAFRALQHEVFQNQ
                     ADATLSVRKTGELTMPEVAEPFAAVITGVAPDVWGRMELAEGRRFSDADFEGAGDVAV
                     INDLLAGQIKKSAPEKSLLGKNIQLGETSFRIVGVLKTQQEGRPEIFVPIVHWTDKEL
                     QKSPPSCLADVHLVEDVQDVKKQIEDWLTKHYPGHSDGFDVATNEYRVNQITEGFMLF
                     RLIMGMI"
     assembly_gap    11113..11127
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11128..11494
                     /locus_tag="PK198_09215"
     CDS             <11128..11494
                     /locus_tag="PK198_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF38958.1"
                     /translation="LVGGIGIMNVLLISVSERTAEIGIRKALGAKRRDILRLFLSESV
                     AISLLGSFLGLLLGMLGAAVAVAIVGMVLKGPQFEAAYTFQTLLIIVAVALLVGIVFG
                     TYPAIKASRLDPVEAMRRE"
     gene            complement(11506..>11826)
                     /locus_tag="PK198_09220"
     CDS             complement(11506..>11826)
                     /locus_tag="PK198_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HRF38959.1"
                     /translation="EADSLKPWEAYYQTVIYLALHLTGSQVACEVQTNRGYIDAVAET
                     KDHVYVMEFKVGKAAEAMQQIKGKGYPEKYLHRGKPVTLLAVGFDPATRNIGDWEEEA
                     VSKH"
     assembly_gap    11827..11836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11837..>12208)
                     /locus_tag="PK198_09225"
     CDS             complement(<11837..>12208)
                     /locus_tag="PK198_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38960.1"
                     /translation="TGWLARKLRETALNVSELEGMVVDESFFDKFEVRNLDPAVLLYQ
                     TGYLSIKKVQGEGIKRYTLDYPNFEVRHSLMSNLFTEYSLRPVSESASLVWALEGALA
                     AKDLPKFISLLRGIFADISNRL"
BASE COUNT         2922 a         3316 c         3242 g         2618 t
ORIGIN      
        1 cggctacatt gggcggcgtg ggctgtaccg ctgccgaggc gcaactcacc gtaccggcgc
       61 ccggcgcagg catactcacc gtcgggccga gaatggtgta ggtgtaagtc caggggtagg
      121 tgttgttctg acagtcggtg taagtccagg tgtaagtttt cgtacctgca caggcagggt
      181 cggggctgat gaccgggccg ctgatctgca tcgggttgcc gcagttgtca tttactacag
      241 gaggcgtcgg cacgttcgct ccgcttgggc aggctacggt ggaggctcca ttgggcggca
      301 tcgggccgcc ggtaccgccg gtgatggtgt aagtgtaaac ccagttgtaa ggatttccgg
      361 cgcaatcggt gtaagtccag gtataggtct ttgtacccgc acaggtcggg ttggggctga
      421 tgaccgcgcc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagttgtc cgtcacgttg
      481 ggtggcgtgg gctgtaccgc tgccgaggcg caacctaccg tgctcgcacc cggcgcaggc
      541 atactcactg tcggggcgga aatggtgtag gtgtaagtcc agttgtaagt ttgactgttg
      601 caggcatctg tgtaagtcca gacataagtt ttaactccca cgcaagccgg gtcaggactg
      661 ataaccgggc cgctccttgc caagaggccg ccgcaactat acatcacgtc gggcggagtg
      721 ggcgccgttg ctgccgatac acacgctacg gtgctgcctg cgtcggtggg catggtaacg
      781 gtggcggagg tggtaaaagc aagcggcgcc gacaaatcgc agcaatgcgt aggttggcac
      841 acaatctggc caatggtggc ggggttggta atggcggttc cggcggtgag ataattgtaa
      901 ctatatactg aatatgtgcc gcaggctacc ggcgtggtga aattgccgga agggatgatg
      961 tacagcactg agcctgagct attggtcaaa atataagttt gcgtatatcc cgaagtcgtc
     1021 tgatgaccgg tagcggaggc ggtgaacatt tcacccgggc attgcggatt cgggttgatg
     1081 gctaccgcgc ctgcattggc agcgcaacag agcatcggcg tcacgatcac attgtccgtc
     1141 gcggtgcaac ctctggcgtc ggtcaccgtg agggtatagg tggtcggcgt ggcaggggag
     1201 gcggtcggat tggcaatacc ggaattgctc aatgttcccg ccggcgacca actgtaagta
     1261 taacccggca ctccctgcgc ggccgccgga gcacccccga tgcccaccga acccggcgag
     1321 cagaaattgg catccgcacc ggcgttggcc gtcagcagcg ggtggttttg gtcgttgtaa
     1381 aaagcaacat gcaggaaaaa ctcgttgggc gacacataca gcggatgccc cggcccgatg
     1441 ttgtaaccct caataatgac tctggaattg gggcccaaaa aaccgttcca aatatcagta
     1501 ggcgtggtgc tcctgggcgg gccgttgctg taatcgaact cgtaggtgcc gtcgccggtc
     1561 tggtccaccc gcaccctgac atgatcgtca aaagcgcgca cattgatata ataataggcg
     1621 ccgcagggga agccgcatcg ctcggcgcga atactgaaag cttcgtttgt caggggcagg
     1681 ccggtataac cgttggcctg gctgggaaag ccgttgaccg gaacgccgtc tttgccaaca
     1741 ttgaactcaa tgtctaaagg atcgttaccg ccgtcgccgg ggctgatgct gtggccgggc
     1801 gtgttgtaaa atcccataaa agtcgtgtag gtattatcgg catacacgct cacattccat
     1861 gtattggagc cgcaagatgg catggccggc gggttctgca ccgtcacagt caccggcgtg
     1921 cgggctgatg cgcagccact gcccgtatca catgctacat aataagtggt agtggcactc
     1981 agcagcggcg ttacgaatgg cgagcctgtt cccacctgtg ttccgcccga tggggcattg
     2041 taccagcgca gggtgccggc acagcctgat gcggtcaggg tacaggaggt attaggaggt
     2101 atcgtttgcc ctgatgcggt aggcgcgggg ggcggcgtgc agggcgtgct gcaagtaaca
     2161 ttggcggccc atccggagta agtcaggctg ccgtcggaac aaaaaacaaa agtgaggcag
     2221 cctccgggat gcgtggaggt aaacgggcct ggaatggacg tgccccaaaa cccgccggga
     2281 gtagagcaaa tgccactgcc ggacccgttg cccgaactga tctgcggcga ggaagcattg
     2341 gggccgttgt acacatacaa tttatcgtag ctgctctctg tgttgaaggc tgtgaatgtc
     2401 accgtcacaa tatcgccggg agtggtgggg caaatggtgg tggtggcatt ttcgttattg
     2461 gagtagttgc cggaaggccc gccggagtcg taaaaaaccg agccgcaggt caaaggcggc
     2521 ggcggatagg tacaggtacc atcgttgacg gttgctgcag ggttgtaatt gagcgcggcg
     2581 gggtcattgc agccgcaaac gatgccggga aacatggggc gaatattggt ggcgataccg
     2641 tcatttgcac caacatggtc ggtaatgcat acttgtattg tatatggagc ccctccgcac
     2701 aatgatgcca gacttgcaaa cgagcttatc atactgttgg cgccacaggg gtctgtcgat
     2761 aatccaaaat tgccgccggt tatcgggatg ccaagatcgg ggttcgggcc gccgccgtcg
     2821 gaaaatgaaa ccaacgcagg gttcccgaca gtgcctatat ctgcgctgct gccgcaagca
     2881 caacctccgg tacaactacc gccgccgggc cttgtgagca acatcagggt gccgccgcac
     2941 gcattgatgc gaatgctcag gtcgccctgc cagtcgtgtt caagcatcat ttgcaaaccg
     3001 atgggatgtc cggtcaccgc gggatctata ttgatggcgg tacaataagg attgggaggg
     3061 ttgagtaccg gttggctgga ggcgtcgggc aggttcagtg taggcccggt acactgtgcg
     3121 ttcagtttcc cgatatgccc cccaaacatc cacaagctgg tcaggaggag tatatagaat
     3181 gcgcatttgg gccgtgtaga attgctcgcc atagtacaca atttgaatgt aataatattt
     3241 tgttgtgatg atgggtattg ggcggagaga tgcagtgccg gaaaattgca aaaaaaatca
     3301 aaagtcggct tgtatatttt ttatgcctct taaaaaatcg aacaaatatc ttccaaatcc
     3361 cataaaatca atttgtatca agcttaaatt ttcacattct cctctgccga agattcattc
     3421 caccgtcact tttcgcagtt gtgttaaaaa atataggccc gaatgttttt tatttcnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgggcagg tatgttggct
     3541 cgcagtgtat tttttcaaca caccaaataa ctaccgtact tatgaacttc gataccagaa
     3601 acattcgcaa cgtggtactc ctgggccatt ccggctccgg caagacaaca ttggccgaag
     3661 ccatgctttt tgaaggcaaa gccatctctc gccggggcaa agtagaagat caatccactg
     3721 tgtccgatta caccgacctg gaacacgagc gcggcaactc catcttcggc accttgctgc
     3781 acgttctttg gaaagactcc aaaatcaata tcgttgatac tcccggctcc gacgactttg
     3841 tgggcgaggt catttcctcc ctaaaagtgg ccgacacagg cgttgtcgcc ctcaatgccc
     3901 gcagcggcgt agaagtaggc actgagctga tctgggaata catcgaaaag ttcaaaacac
     3961 ccgccctgtt tgccatcaat cacatagacc acgaaaaggc cgatttcgat actaccctcg
     4021 aacaggcccg tcaacgcttc ggcaatcatg tcattccggt gcaatatccg ctcaatcccg
     4081 gcgccggttt taataaaata gtggacgccc tccgcatggt catgtatgtc ttcccggaag
     4141 gcggcggcaa acccgacaaa caacctatcc ccgcttccga acaggaacgc gccatggaaa
     4201 tgcacaacgc attggtggag gctgccgccg aaaacgacga gggcctcatg gagcgcttct
     4261 tcgagcaggg cacgctctcc gaagaagaac tcacagaagg cctccgcatc gccattgcac
     4321 accagcaaat tttccccgtg ttctgctgct cggccctcca ggatatgggc agcggccgca
     4381 tcatgggctt catcaatgac gtgtgcccct cgcctgccga ccgccccgct gccgaactcg
     4441 aaggcggtgg aactctcgcc tgcgacagca aagacagcgc tacggttttt gtttttaaaa
     4501 ccgtttccga gccgcgcgtg ggcaaccttt cctactttaa agtttactcc ggcgtactcc
     4561 attccggcga cgaactcgtc aatgtggaaa accgcagtgc agagcgcttc ggccagatat
     4621 acttggccaa cggcaaagac cgcgagggcg tcaatgcact catggccggc gacatcggcg
     4681 tcaccgtcaa actcaaaaat acgcacacca acaatacact caatatcaaa ggagtagatc
     4741 gaaaaataga cccgatccac ttccccaccc cgcgcataga cgtgggcgtg catcagccca
     4801 acaaaaacga catggaaaag ctgatgaaag ccctccatac catcgccgag gaagacccga
     4861 cgctcatcat tgagcaatcc aaagccctca aacaaacgct cctttacgga cagggccaac
     4921 tccacctcga cctcatcaaa caccgcatcg aaatggtgta tggcgtcagt atggagtatg
     4981 tgcgcccgcg cattccttac cgcgaaacca tcacccgggt ggccaacacc atgtatcgcc
     5041 acaagaagca aaccggcggc gcagggcagt ttgcagaggt tcacatgcgc atcgaacctt
     5101 atcatgaagg catgcccgct cccaccgacc tatctgtgaa aaacgtagag gtggacgaac
     5161 tgccctgggg cggcaaactc gtctatcagt ggtgcatcgt aggcggcacc attgacgccc
     5221 gtttctccaa cgccatcaaa aaaggcatca tgaacaaaat ggaagaaggc ccgctcaccg
     5281 gctcttcctg ccaggacatc cgcgtcagca ttttcgacgg caaaatgcac cccgtggatt
     5341 ccaacgatat ggccttcatg gtagcctccg tacaggcatt ccgacaggct ttcgccgaag
     5401 cggcgccaca actgctcgaa cccatccacg acgtagaaat tctctgctcg gacgaggtca
     5461 tgggcgatgt catgggcgat ctccaaaccc gccgcgccat catcatgggc atggattccg
     5521 acgggcacta ccaaaaaatt ctggcgcgcg tgccactggc cgagctgtac ttgtactctt
     5581 catcgctgcg ctcgttcacg cagggccgcg ccaagtttac ccgcgctttc gcagagtacg
     5641 ctcaggtacc cggcgatatt cagcaaaggc tcatcgaggc acacaaagcc gagacggagc
     5701 acgaggcata aaccagccaa aatcagtttt tctctcagcc ctgtcggagc acttccggca
     5761 gggctgattt ttttataaaa acaaaacatt ttgttttatt tttataaaaa aacaattaaa
     5821 tttgccatct aattcggcat ggttgttcaa aacagcccgt tgcacacagc tctcaaaaca
     5881 tcatcttatc attttcaacc aaaactctct ttatcatgaa agcaatcaaa attgcgggca
     5941 ttttgctcct cgcactcatc gtactggtac tcgtactcaa tgcactagca ccgaaaacct
     6001 ttgaaatgga gcgcagcatc gtcattgatg cgccccgcga aacggtattc ccttatgtgc
     6061 agtatctgga acaacagagc cgatggtcgc cctggagtcg gttggacccc aacatgaaaa
     6121 ccacgctcac gggcattgac ggaaccgtag gcgctgcctc tgcctgggac ggcaacaaag
     6181 acgccggaaa aggctctcaa acgctcagcc tggtgcagcc aaacgagcgg gtagaaacgc
     6241 agttggcttt catcaagcca tttgaatcca ccgcccaagc ctggtttcaa ctcaaagatg
     6301 ccgaaggcaa ttccacggag gtaacctggg gatttcgttc gccaatgcct ttcccgttca
     6361 atgttttcgg actgattatg ggcatggaaa aagaaatgga tgcccaatat acgcaagggc
     6421 tggccgatct taaagcattg gtagaaaaag aggctgccgc cgctcctgcg ctcaaagtga
     6481 tggaaagtnn nnnnnnnngg ccgctacgcc ccgatactat gtgggcatcc gcgaaacagt
     6541 ggccattccg caattgacag aacgtatggc cctgcatttc cccaaaattg ctcaagcctt
     6601 gcaagcggcc ggcatcgaat tggacggaat gccctccggc ctgtatttca cctgggatga
     6661 ggcagcgcaa accaccgact ttgccgctgc atttcctgtc aaatcgaaag tggagatcaa
     6721 ggggttcact actgttgaaa tgcccgccgg tacgcgcttg tcggtggact actacgggcc
     6781 gtacgaaaaa atagctccgg cgcatgaggc cattgacgcg tatattcagc gcaacaaatg
     6841 gcaacacggc gctatggctg tggaggaata cgtcaccgat ccgggcatgg aacccaaccc
     6901 ggccaaatgg ctcactaaag taacgtatcc ggtaagtaaa taaagctttc ctgccggaac
     6961 aggaagggct tttcgatgtt tgaagcattg gaaagccctt tttgttttta agcgcctgtt
     7021 tccggtaacc ttttttatta tttgtaaata aacaactacc acaaaacatg tattttgtgc
     7081 tgcgatattg atcactgctc ccaaacacta tgctgagaaa acacttatat tcgttcctgt
     7141 tcctgttgac aggactatcg gcagccgctc aaaacgaatc tgttgccacg ctgaaagccc
     7201 ggctggaaag tgccaaagac ccggccaagg tcatacagtt actgggtgtt ctggctgatt
     7261 ctacccaaaa atcagacgta caagccttgc gtccgctggc gcagcaagca ctccggctgg
     7321 ccggcggcaa cctctccgac actcttaaag cccggactta cctcattgcg ggcaaggctt
     7381 tggcgcagat ggtaagttat gattccgcag ccatgtttct ggatcgttct ctggccttgc
     7441 ttgatccctc cagacaagcc gcagaaaccg ccgaagccaa tcactggaaa ggatttgcca
     7501 aagccaatca gcgggagttc gctgcggcat cagagcatta ttatcaggca gtagaaattt
     7561 gggagcgcat cggtcgccgc agagcattag cagaaactta caatcaattg tcggatatgc
     7621 acgccatgca ggaagactac gagaagtcta tccattatgc ccaacaagcc attgcccagt
     7681 ttgaatccat ggacgcgccc gaactactgg ccgatgcgct caataacctg tcttacgtcc
     7741 atgtcctcaa aggcgactac gcaggcgcgc tgcaatacgc tacggaagcc ctgcaaatat
     7801 gggagcgcat tgcgcccgac gggctgcaag tggccagaac atccaacagc aggggcaacg
     7861 cacacaaatt tttgggcaat tacgaagaag cccttctcga ctttgagcgc tgccggagca
     7921 ttgccgaaaa gttgggtttt gtgagaggcg tcattgccgg cacggccaac aagggccatg
     7981 tactgttgat gcaaaagaag tatgcagagg cattgcccta tactttgcgg gccatagagt
     8041 tgatgaagga atcgggggat acccgcaatc tgtgggaaaa ctacatgcac ctttccaata
     8101 tttacgccgg catgggcgac tacccaaacg cgcaccgcta ccaacagctt caccaagagg
     8161 aaaaggagcg cgaatacgag caaaaaatag ctgccttgca gggagggtta gccgaaaaat
     8221 acgaggccgg gcaacgggca gccaccattg ccatacagaa ggagcgcatc gaaaaacagc
     8281 gcaatttcca gcggctcctt tggggggttg cgggcttgct gctgctcact tctgtcttga
     8341 tttacatgag tttgcgctcc cgacagaagg ccaatcgcct gttggaagct gccaatgaac
     8401 aattggcaaa aaagaaccgg gaaaatgagt tgctcctgcg cgaaatacac caccgcgtaa
     8461 aaaacaacct ccaaaccatc tccagcctgc tcaacctgca aagtgccggc atagaagacg
     8521 ccgccgcctt agccgccgta caggaaagcc gcaaccgcgt gcgatccatg tcgctcattc
     8581 atcaaaaact ctatcagggc gagcaattgg cctcggtgga aatgaaagac tattttgaga
     8641 ccatgggcca ggccatgctc caaagcttcg gggcagcagg cgagcagata gcgctcaaag
     8701 tagatatgcc gaccatagac ctcgatgtgg ataccgccat ccccatcggc ctcattgtca
     8761 acgaattgat gaccaactcc ctcaaatacg ctttcccgga acaccgcggg ggcgccatcg
     8821 agatcagcct gcgcgccgaa gacgagcata cgctggccct gcgcatagcc gacaacggcg
     8881 tgggtggcaa ctcggagggc ggctcggaat ggcgcgaatc aagtacaggt tttggaggaa
     8941 aacttgtcaa attgctcgca caacaactcg gcgcccggat ggagcaaagc gagaccgggg
     9001 gctacaccac catcctccga tttcccaatc ctgcaaaagc cgcctgacgc atgagcgctg
     9061 ttaaaatcct catcgtagaa gatgaaatga tcatcgccgc cgacatctcc atgatgctgg
     9121 aaagcatcgg atatgagatc accggcatca tgccgcgcgg cgaggatgcc ctcaaaagta
     9181 tagagagtac gccccccgac ctcgtgctga tggacatttc gctcaaaggc gtgctcgacg
     9241 gcgtagccac ctcgcagttc atttacgaac gccatcagat tcccgttatc ttcctcactg
     9301 ccaacgccga cgacgccacc tttgcccgcg ccaaagccac acgcccttac gccttcattt
     9361 ccaaaccatt ccagcccgcc gacctccagc gtgccatcga attggcactg gcccgattgg
     9421 ccgaagagca cagtggccaa agtctgctgc atgtaaacga caaggccgaa gatgcctaca
     9481 ttctggccga ccgcatcttt gtgcgctaca aaggcaaaat gaccaaaatc ctgcttcagg
     9541 acatcctcta cgccgaagcc gaccgcagct attgcaaaat ctatacgcca caggccgagt
     9601 atctgatgag catctcgctg ggtgctttgg aggaaaaact tcgcaccgac aacttcctcc
     9661 gcattcaccg ttcgtacatt gtcaatctgc acaaaataga taccatcagc gacgactggg
     9721 gctacctcgg catcggcgcg cagaccctct ccgtgagccg gtctttcaaa gacgaattag
     9781 tgcgccggtt tcatattatt tgagcagcgc agcatttcat tcggaacccc aaacccatcc
     9841 ttcggaaact caaacccgtc attcggaaac tcacccttgc tgcaaagaac agcgtgttgc
     9901 aattttattg cgtcaatcgg ggtaaccatt cggaagcatc ccaaatagac accacacatc
     9961 cggcgcagca cgaatgaact tgtgtcaata acaatctttt tataaacatc caaaacccat
    10021 attgcctgcc gttcagcttg ttgttgcatg gctgacggca ggttcttttg gcctgtagga
    10081 tcaaaattca gcaaacctcg gcagcagcct ccgctcggaa gggctgaaca ttaccagcct
    10141 gcccctaaac ttcacagaac ccgacgccat ccatagcctc tatctttatt tgccgtgctt
    10201 acatctaaaa cattgcccct tcccactact tttcttactt tctttgcagg caaaccatct
    10261 gccatgaaaa agttgtcggc caccatcggc gaggcattcg agagcattcg cgcccgcctt
    10321 ttccacacct tgctttccgt attgggcatt gtcatcggcg ttgctgcctt agtgaccatc
    10381 ctctccctca ttgacggcat ggaaaagcac gcccgccgac aactgagcga tacgacctcc
    10441 gtgaaaatga ttcaaatcag ctcagtgacg accaaaaccg tgcagggtat gaacatttcc
    10501 aaagattcct tcccatatct caattatgag gcgttccggg ctttgcagca cgaagtgttt
    10561 caaaatcagg ctgatgcaac gctcagtgtc agaaaaacag gcgagttgac tatgccggaa
    10621 gttgcagaac ccttcgccgc agtcatcacg ggcgtagcgc cagatgtgtg gggccggatg
    10681 gagttggcgg aaggccggag attttcggat gccgactttg agggcgccgg agacgtggcg
    10741 gttattaacg acttgctggc cggccaaatc aaaaaaagtg cgccggaaaa aagtctgttg
    10801 ggtaaaaaca tccagttggg tgaaaccagc ttccgcatcg tgggcgtgtt gaaaacccaa
    10861 caggaggggc gccccgaaat ctttgtcccc attgtgcatt ggaccgacaa ggaacttcaa
    10921 aaatcgccgc cgtcctgttt ggcggatgtt catttggtag aggatgttca ggatgtaaaa
    10981 aaacagatag aggactggct cacaaagcat tacccaggcc acagcgatgg atttgacgta
    11041 gctaccaacg agtaccgggt caatcagatc acagagggct tcatgctctt tcggctcatc
    11101 atgggcatga ttnnnnnnnn nnnnnnngtt ggtgggcggt atcgggatta tgaacgtgct
    11161 gctcatttcg gtatcggagc gcacggcgga aatcggcatt cgcaaagcat tgggcgccaa
    11221 gcggcgcgac atcctgcggc tgtttttgtc ggaatcggtg gccatatcgc tgttgggcag
    11281 ttttctgggg ctgttattgg gtatgctggg ggccgcagta gcggtggcga tagtaggaat
    11341 ggtgctgaag gggccgcaat ttgaagccgc ttataccttt caaaccctgc tcattattgt
    11401 ggcggtagca ttattggtgg gcattgtatt cggcacatat ccggccatca aagcatcacg
    11461 gcttgatccg gtggaggcta tgcggaggga atgaagtatt ggtaatcagt gctttgacac
    11521 cgcttcctcc tcccagtccc cgatattgcg cgttgcagga tcgaagccca cggccagcag
    11581 cgtcacgggt ttgccccggt gcaggtactt ttcggggtag cctttgcctt tgatctgttg
    11641 catggcctcg gcggctttgc ccactttgaa ctccataacg taaacatggt ctttcgtttc
    11701 ggccaccgcg tctatgtagc cccggttggt ttgtacctcg caggccacct gactgcccgt
    11761 gagatggagc gccaggtaga tcaccgtttg gtaatacgct tcccagggtt tgaggctgtc
    11821 ggcttcnnnn nnnnnnaggc gattggaaat atcggcaaag atgccccgca gcaggctgat
    11881 gaatttgggc aggtcttttg ctgccaaagc tccctccaat gcccatacca aactcgctga
    11941 ttcgctcaca ggtctcaggg agtactccgt gaaaaggttg ctcatcagcg agtgccgcac
    12001 ctcgaagttg ggatagtcta aggtgtagcg ttttatgcct tctccctgta cttttttgat
    12061 gctgaggtag ccggtctggt acagcagtac ggcggggtct aaattgcgca cttcgaactt
    12121 atcgaagaaa gattcatcca ctaccatgcc ttcaagttcg gaaacattga gggcggtttc
    12181 gcgcagtttg cgggccagcc atccggtg
//
LOCUS       DAORLF010000089        12090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2812_length_12090_cov_2.061072, whole genome shotgun sequence.
ACCESSION   DAORLF010000089 DAORLF010000000
VERSION     DAORLF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_12090_cov_2.061072"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2942
                     /gene="sprA"
                     /locus_tag="PK198_09230"
     CDS             <1..2942
                     /gene="sprA"
                     /locus_tag="PK198_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF38961.1"
                     /translation="IKVRGNPNFGLAKQIMVGVRNVPDGNAGPFDFETWINELRLNGL
                     DERAAGAAVARADLQIADLASVTVAGNFSDIGFGALNQKVAERSREAIYGYDFSTNVQ
                     LGKFLPNKWGINLPLYAQVSNNTRRPEYDPYDLDVPLKVKLANAADQATRDSIRNLTL
                     DVTQLRTVNFTNVRKERAPGNAQPKPWDIANFSANYSHTVTDRRDPLIEFDTEERSTG
                     GIDYTYSRPVKYLEPFKNLIKKDKYLKFITALNFNPIPNSVTFSTILNRQFAETRYRF
                     TGLDDRFNTFYNKRFNWDRNFNLQWDMAKSLKFNFASRTNAVVDEPDETWMQENIDIS
                     EIPRFRRDSILNSLRDFGRTKNYNHNFSLNYTLPTKTIPFMDWIQVRAQYQAEYNWTA
                     IALNVDSLGNVAQNKQRRQLNADFNFDNLYAKIGYLKRIERGVPPAKTKADEKEKGTR
                     TKSEEPADKNATADKNATADKKPKDDVPSALERALIRPLLMIRKVRLSLAEDFSTSLP
                     GFMQQAGLLGMGKGFDGPGWDFIAGLQPRIRTLNENQYYTSSDWLHEVGNNRRWISNS
                     VFQNREVLQDYSRRFDGRLTLEPYRDFRIEVELNSAFAEGHSEFFKDTLVDGNFNLIH
                     AVPKKSGSMTVSYFALGTPMSTDSTVIRRLFRQFDHNRVIVSQRLGTGQHEIDSLAAR
                     GYTRGFGNIQNEVLLPAFIAAYTGQDARTIGLDVFKTLPLPTWRLTYNGLTKLPGFRD
                     IFQNFSLTHGYKSTLSVNRFDTNPLWLDTRNLNGTDPFSGNFYARLLIPEVVIQEAFS
                     PLIAIDATLKNGMSFNIDYKKSRTLGMSFISYQLAETQSKEISIGFGYLLRDVDIPFL
                     TGSNKKGAKKPDPKKPDPNQQGQQQQRQQQGGRGGQLRSRDLDFNFNFSLRDDVTFNH
                     LLDQGVVEPVRGNYALSISPSAEYQINTRLGLRLFFDYRQNIPRTSAGFPRTDTSGGL
                     VVRFKLQ"
     gene            3138..5093
                     /locus_tag="PK198_09235"
     CDS             3138..5093
                     /locus_tag="PK198_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRF38962.1"
                     /translation="MQTDRRNRVIIQNVQPEIDDGRHFIKRVVNEWTQVTADIFSDGH
                     DAVRASLHYRHESNKNWTEAYMEAQPNDVWTAAFQVTQKGFYHYKLEAWVDHLLNWHN
                     GFLKKQADRQDMAVELLIGADLLKRTAKGYPKAKAAALNKAAELLEKKGAYDEATAAV
                     LSQEFADMVQAHPMKQFQTWYDKDLRVRIGRDRAVFSAWYELFPRSAAAKPGDHGTFK
                     DVERILPRLQELGFDVLYLPPIHPIGTSNRKGKNNSLDTGPHEPGSPWAIGNTDGGHT
                     AIHKQLGTLKDYQHLIAEAQKHGLEIAFDLALQCSPDHPWVKEHPEWFLWRPDGTIAY
                     AENPPKKYQDIVPINFETEDWRNLWEELKKVILFWVDAGVLIFRVDNPHTKPFAFWEW
                     VINEVHSQHPDVIFLAEAFTRPKIMGALGKLGFSQSYTYFTWRNNRREMEEYLTELTQ
                     TEWRDYYRPNFWPNTPDILPYHLMDAGANVFVLRLLMAATLSSNYGVYGPSYEFMENK
                     GNTNGKEEYLDSEKYELRTYDWNARNRITEAMTAINRIRREQPALQRTFNLTFTRADN
                     ESLMSFVKVSDDGSNVIWCVVNFDAQYTQSGYVEVPKKLLGIEGRVNLKVTDLLTEET
                     YHWFNDWNYVEINPNKFPMHLFWVSMR"
     gene            5272..7380
                     /locus_tag="PK198_09240"
     CDS             5272..7380
                     /locus_tag="PK198_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38963.1"
                     /translation="MKLASTLGLLLLFLLGFNTATQAQATTCSYTLEMFDSFGDGWNG
                     ATLIVNIGGVITPYTVTEADNDGFSRTVTLTVTNGAPISFLYTPGIFEFEVTYRLRDS
                     DGNIIFSDGPNPDLGVVFTTTAVCPTCPALSAAAVGNLDIRAFTARIGWVPSDTLGVY
                     RIEYDTTGFTLGTGNVKLAPGRMTTLTGLRENTDYQYYITVLCANGDTSTTVGPFTFR
                     TLWANNVGVTELISPISGCALSATEEMRVRIRNFGGNPQSLIPIKFSVNGQVAPVNIP
                     LDGFYTGVLGKDSTDTFLYDLTWDFSEPGEYIIRIWTDLQSDSLRANDTLTVVINSLP
                     VIDNLPYLEDFETWNGGWRPSVAGTNSSWEFGVPAGALLNAAASGQNAWVTNLQGEYN
                     NSELSYLESPCIDFSGLTQDPRLLFSLWFDSEACCDEAWVEQSINGGQTWTKVGASGT
                     GLNWYNDNLNQWWNGTGGFNGWVTAFNTLTGTAGQSDVRIRFVFSSDGSVVREGMGVD
                     NIFISQPVARDLLNQGATYTAAAACGTETGTVRINVVNLGTQAQTGFNVAYRINGGAP
                     VVENVGALNIPPNQQAAYTFTTPLNFTQNGTYNIQLWTELAGDLQLSNDTSSLIVNVE
                     LVPVIVGLPYFTNYENGRDGWRVGIGSAASSWQFGPPAAPMLNAAASGQNAWVTNLAG
                     QYNNNELSYLVSPCYDFSSA"
     gene            7356..>8226
                     /locus_tag="PK198_09245"
     CDS             7356..>8226
                     /locus_tag="PK198_09245"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRF38964.1"
                     /translation="MLRLLQRLSFSMWFDSEACCDEAWVERSIDGGQTWTKVGASGTG
                     LNWYNDAGSQWWDGTGGFNGWVTAFNTLAGVAGQANVRIRFVFSTDGSVTREGMGVDD
                     VRISIPAAADISNAVTAYTAASSCGVENGTVRLTIVNFGTQAQTGFNVAYSVNGGTPV
                     VENVGALNVPANQQATYTFTTPVQFTQNGSYNITAWTELAGDEALSNDTTGITVNVEL
                     VPVIPDFPYTNNFESGNGGWKVGAGSNSSSWQFGQPVGALLNSAASGINAWVTNLAGA
                     YNTNELSYLVSPCF"
     assembly_gap    8227..8241
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8242..8840
                     /locus_tag="PK198_09250"
     CDS             <8242..8840
                     /locus_tag="PK198_09250"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF38965.1"
                     /translation="MDPFLSFSLWFDSEACCDEAWVERSIDGGQTWAKVGTSGTGQNW
                     YNDDGNQWWDGTGGFTGWVTAYNTLTGVAGQSNVRIRFVFSSDGSIQGEGIGVDDINI
                     DATPTALVEPIDRLTGISLAPNPTGGVSILSVSFAEPVDAIISIHDMLGRQVSVIKDQ
                     RVSNGAYPIDLSNQPAGIYLVRVMVDGKVNTLKLVRGQ"
     gene            8953..9585
                     /gene="bshA"
                     /locus_tag="PK198_09255"
     CDS             8953..9585
                     /gene="bshA"
                     /locus_tag="PK198_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRF38966.1"
                     /translation="MKIGIVCYPTYGGSGVMATELGLGLARHGHQVHFITSRRPPRLS
                     TFHENVYFHEVSGEDYPLFEYPPYDTALASKMVDVVKFENLDLLHVHYAIPHATVAYI
                     AKKILLTQGRYVPVVTTLHGTDITLVGSNKAFAPVVEFSINKSDGVTAVSESLKQETC
                     EYFDIQRDIRVIYNFIDFARFSKSDKDHFKKAIAPNGERIIVHTSENANA"
     gene            9600..10088
                     /locus_tag="PK198_09260"
     CDS             9600..10088
                     /locus_tag="PK198_09260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF38967.1"
                     /translation="MFRRIHEHTPSKLLLIGDGPERYHLEELCRRMDLCHEVRFLGKQ
                     DAIEELLAVSDLFVMPSESESFGLAALEAMACEVPVISSNAGGLPEVNIHGQTGYLSE
                     VGDVESMARNALQILNDDDTLRRFRDAALEQARRFDISIILPQYEQYYEEVLAASALR
                     VE"
     gene            complement(10169..>10953)
                     /gene="nqrF"
                     /locus_tag="PK198_09265"
     CDS             complement(10169..>10953)
                     /gene="nqrF"
                     /locus_tag="PK198_09265"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRF38968.1"
                     /translation="IECDYSTIDITSHPRMGKAPEEFRPEWDKFKLWGLKMKNEEPIF
                     RAYSMANHPAEGNIVMLNIRVATPPFDRATNGWMNVNPGICSSYVFTRKPGDKVVVSG
                     PYGEFFIKPTKKEMVYIGGGAGMAPLRSHLFHLFHTLKTTDRKVSFWYGGRTRRELFY
                     VEDFRAIEREFPNFKFFIALDNPLPEDNWVAKKDIHDTEGEGFVGFIMPVLYEKYLKD
                     HPEPEEVEYYFCGPPMMNQSVIKTLDELGVPQENISFDDFGG"
     assembly_gap    10954..10988
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10989..11246)
                     /locus_tag="PK198_09270"
     CDS             complement(<10989..11246)
                     /locus_tag="PK198_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRF38969.1"
                     /translation="MCKCHVDAGGGDVLPTEMNHFTRKDVAENLRLACQVKVRQDMRI
                     RVPEEIFGIKKWECEVVSNYNVATFIKEFVVKLPEGEHLHFE"
     gene            complement(11197..>11578)
                     /locus_tag="PK198_09275"
     CDS             complement(11197..>11578)
                     /locus_tag="PK198_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRF38970.1"
                     /translation="VASLATTPFAAYHFDRFQLYGIVANLIAVPVTSFWIMPCAVPAM
                     LLMPLGADGPLLQALAWGVSVVVWIAGVVAGWPGAVVLLPPMPAASLALITLGGLWLC
                     LWRRRYLCHVQMPCGCRRRRCIAH"
     assembly_gap    11579..11588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11589..>12090)
                     /locus_tag="PK198_09280"
     CDS             complement(<11589..>12090)
                     /locus_tag="PK198_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRF38971.1"
                     /translation="GIPPATMDAIRASGLAHLLSISGLHIGLVAGIVFVTARSLLALI
                     PWLALRWPTKKLAAILSVIAAAFYTLLAGAPVPAQRSFLMVAIALLAVLVDRQGISMR
                     LIAWAALAVLLTQPDSLTGPSFQMSFAAVVALVATYEAVRARRERRPGERRTVYRILL
                     YPAGIIL"
BASE COUNT         2893 a         3543 c         3081 g         2513 t
ORIGIN      
        1 gcatcaaagt acgaggcaac cccaacttcg gattggctaa gcagatcatg gtgggcgtgc
       61 gcaacgtacc cgacggcaat gccgggccgt ttgattttga aacctggatc aatgaattgc
      121 gcctcaacgg tttggatgaa cgagccgccg gcgctgccgt agcgcgtgcc gacctgcaaa
      181 ttgcagacct ggcctcggtt accgtggccg gcaatttcag tgatattggc tttggcgcgc
      241 tcaaccagaa agtggccgag cgcagccggg aagccatcta tggctacgat ttctctacca
      301 atgtgcaact tggcaaattc ctgcccaaca aatggggcat caacctgccg ctctacgcac
      361 aggtttccaa caatacccgc cggccggaat acgatcccta cgacttggac gtgccgctga
      421 aggtaaaatt ggccaatgcc gccgatcagg caacccgcga ctccatccgc aatttgacct
      481 tggatgtgac ccaattgcgc accgtcaatt ttaccaacgt acgcaaagag cgcgcgcccg
      541 gcaatgccca acccaaaccc tgggacatcg ccaacttcag cgccaactac agccacaccg
      601 tcaccgaccg ccgcgatccg ctcatcgaat tcgacacaga agagcgcagt accgggggga
      661 tagactacac ctactcgcgt ccggtgaagt atctggagcc gttcaagaac ctgatcaaaa
      721 aggacaagta tctgaagttt attaccgccc tgaacttcaa cccgattccc aattccgtca
      781 cgttcagcac cattctgaac cggcagtttg cagaaaccag atacagattt accggcttag
      841 acgaccgctt taataccttc tacaacaagc gtttcaactg ggaccgcaac ttcaatctgc
      901 aatgggacat ggccaagtcg ctcaaattca actttgcctc gcgtaccaat gccgtagtgg
      961 acgaacccga tgaaacctgg atgcaggaaa acatagacat cagcgaaata ccccgtttcc
     1021 gccgcgacag cattttgaac agcctgcggg attttgggcg taccaaaaat tacaaccaca
     1081 atttcagcct caactacacg ctgcccacca aaaccattcc ctttatggac tggattcagg
     1141 tgcgcgcaca gtatcaggcc gagtacaact ggacggccat tgccctcaac gtggacagcc
     1201 tcggcaacgt ggctcaaaac aaacagcgcc gccaactcaa cgccgatttc aacttcgaca
     1261 acctgtacgc caaaatcggc tacctcaaac gcatagagcg aggcgtgccg ccggccaaga
     1321 ccaaagcaga cgaaaaagaa aaaggaacgc gaacgaaaag tgaagagcct gccgataaaa
     1381 acgccaccgc cgacaaaaac gccaccgccg acaaaaagcc caaagacgat gtgcccagcg
     1441 cattagagcg cgcactcatt cggccgctgc tgatgatacg caaggtgagg ctctcgctgg
     1501 cagaagattt ctctacctca ttgcctggat ttatgcaaca ggcgggactg ctgggtatgg
     1561 gcaaaggctt cgacgggcca ggctgggatt tcattgccgg tcttcagccc cgcatacgca
     1621 ctttgaacga aaatcagtat tatacctcct ccgactggct gcacgaggtg ggcaacaacc
     1681 gccgctggat cagcaattcc gtgttccaaa accgcgaagt actgcaagat tacagccgcc
     1741 gtttcgatgg ccgcctcacg ctggaaccct accgcgattt cagaattgag gtagaattga
     1801 acagcgcttt tgcagaaggt cacagtgagt ttttcaaaga cactctggtg gacggcaact
     1861 tcaacctcat acacgccgtg cccaaaaaat cgggttccat gacggtgagc tactttgcat
     1921 tgggaacgcc catgagcacc gattctaccg tcattcgacg cctgttccgg caatttgacc
     1981 ataaccgggt catcgtttcc cagcgcctcg gcaccggcca gcatgaaatt gattcattgg
     2041 cagcgagagg ctacacacgc ggtttcggca acatccaaaa cgaggtactg ctgcccgctt
     2101 tcatcgccgc ttacaccggc caggatgccc gcaccattgg cttggacgta tttaaaacac
     2161 tgcctttgcc cacttggcga ctgacttaca acggcctgac gaaattgccg ggtttcagag
     2221 acattttcca aaacttcagc ctcacgcatg gctacaaatc cacgctgagc gtcaatcgtt
     2281 tcgataccaa cccactgtgg ttggacaccc gcaacctcaa cggcaccgat ccgttcagcg
     2341 gcaacttcta tgcccgcctg ctcattccgg aggtggtcat tcaagaagca ttttcgccgc
     2401 tcattgccat tgatgccacg ctcaaaaacg gcatgtcgtt caatattgac tacaaaaaat
     2461 cgcgcacgct gggcatgagc ttcatcagct accagttggc cgagacgcag tcaaaagaaa
     2521 tctccattgg ctttggttac ctcctgcgcg atgtggacat ccccttcctc accggcagca
     2581 ataaaaaagg cgccaaaaag cctgatccga aaaagcctga ccccaaccaa cagggccagc
     2641 aacaacaacg ccaacagcaa ggcgggcgcg gcggccaatt gcgcagccgc gacctcgact
     2701 tcaacttcaa tttctctctg cgcgacgacg tgaccttcaa tcacctcctc gatcagggcg
     2761 tagtggagcc ggtgcggggc aattatgcgc tcagcatttc gccttcggct gaatatcaga
     2821 tcaatacgcg attaggtttg cgcctgttct tcgactatcg ccaaaacata ccgcgcacat
     2881 cagccggctt cccgcgtacc gatacttcgg gcggccttgt ggtgcgattc aagttgcagt
     2941 aaatagaaac cgagtgaagg agccgtcgta acgtcgctcc ttcactccgc caaaagatca
     3001 gagctaaaaa gggggctgtc tgctgtcgct caagagcaat ctgccctgcc aatcgccact
     3061 ataggacttg tatttcccca aaaatcccta ttattgctgc cgatcttttg aacgcattca
     3121 acaaaccttt gaaaatcatg cagacagatc gccgcaaccg agtcattata caaaacgtgc
     3181 agcccgaaat tgacgatggg cgccatttca tcaagcgggt agtcaacgaa tggacgcagg
     3241 tcaccgcaga tattttttca gacggccacg acgccgttcg cgcctcacta cactatcgcc
     3301 acgagagcaa taaaaactgg acggaggcat acatggaggc ccagcccaac gacgtgtgga
     3361 cggctgcttt tcaggtcacg caaaagggat tttaccacta caaactggaa gcctgggtgg
     3421 accacctgct caactggcac aatggctttt tgaaaaaaca agccgacagg caggacatgg
     3481 cggtagaact cctcatcggc gccgacctgc tgaaacgcac ggccaaaggg tacccgaaag
     3541 ccaaagccgc cgccctgaac aaagccgccg aactattgga aaaaaaaggc gcttacgacg
     3601 aggctacagc cgctgtcctc agtcaggaat ttgccgatat ggtgcaggcc caccccatga
     3661 agcagttcca aacctggtac gataaagacc tccgcgtgcg catcgggcgc gaccgggccg
     3721 tgttcagcgc ctggtacgaa ctgtttccac gctcggcagc cgccaaaccc ggcgatcacg
     3781 gcaccttcaa agacgtggag cgcatcttgc cccgcttgca ggagttgggt tttgatgtgc
     3841 tgtacctgcc gcccattcat cccatcggaa cctccaaccg gaagggcaaa aacaacagcc
     3901 ttgataccgg cccgcacgaa cccggctcgc cctgggccat cggcaataca gacggcggcc
     3961 acacggcgat tcataaacaa ttgggtacgc tcaaagacta ccagcacctc atcgccgagg
     4021 cacaaaagca tggtttggaa atagctttcg acctggccct gcaatgctcg cccgatcatc
     4081 cctgggtgaa agagcacccg gagtggttcc tctggcggcc cgacggcacc attgcttacg
     4141 cagaaaatcc gccgaaaaaa tatcaggaca ttgtgcccat caactttgaa accgaagact
     4201 ggcgcaacct ttgggaagaa ctcaaaaagg tgatcctgtt ttgggtggat gccggcgtcc
     4261 tgattttcag agtggacaac ccgcatacca agcccttcgc tttttgggaa tgggtcatca
     4321 acgaggtaca ctctcaacat cccgacgtca tctttctggc ggaggccttc acccggccca
     4381 agatcatggg cgcgctgggc aaactgggct tttcgcaatc ttacacctat ttcacctggc
     4441 gcaacaaccg ccgcgaaatg gaggaatacc tcaccgaact gacgcaaacc gagtggcgcg
     4501 attactaccg gcccaatttc tggcccaata cgcccgacat tctgccttac cacctcatgg
     4561 atgccggcgc caatgtattc gttctgcgcc tgctcatggc ggccacgctc tcgtccaact
     4621 atggcgttta cggcccgtct tacgagttta tggaaaacaa aggcaacacc aacggcaaag
     4681 aggaatattt agactctgag aagtacgaat tgcgcaccta cgactggaac gcccgcaacc
     4741 gcatcacaga ggccatgact gccatcaacc gcatccgccg cgagcagccc gccctgcaac
     4801 gcaccttcaa cctcaccttc acgcgcgccg acaacgaatc gttgatgagc tttgtaaaag
     4861 tgagcgacga cggcagcaat gtgatctggt gtgtcgtcaa ttttgatgcg caatacacgc
     4921 agtcggggta tgtggaagtg cccaaaaaat tgctcggtat agaagggcgc gtcaacctga
     4981 aagtgacgga tttgctgacg gaggaaacct accattggtt caacgactgg aactatgtgg
     5041 agatcaatcc caacaagttt ccgatgcacc tcttttgggt ttccatgcgt tgatgcggcg
     5101 cgtttctgcg gccgcttttg agcgtgatgc ccattgggga aaccctgtgg tgcgtcgtcg
     5161 tttgcccgac aaaaagcgcc ctgttttgaa aaacaggctt gctgttttga tttttttcta
     5221 attttagcct ctcatctgaa tcgaaaatct catcggaaaa gcacactatc tatgaaatta
     5281 gcttctacac tgggtctttt actcctgttt ttgcttggat ttaatacagc aacacaggca
     5341 caagctacca cttgtagcta cacgctggaa atgttcgact cctttggcga cggttggaac
     5401 ggggccacgc tcatcgtcaa tatcggcggc gtcatcactc cttacaccgt gacagaggcc
     5461 gacaacgacg ggttctcgcg caccgtgacg ctcaccgtga ccaacggcgc tcccatttca
     5521 tttttatata cgcccggcat cttcgagttt gaagtcacct atcgcctgcg cgacagcgac
     5581 ggcaacatca ttttcagcga cggcccgaat cctgatctcg gcgttgtttt taccactacg
     5641 gcggtttgtc ctacctgccc ggcgctttct gctgctgcgg tcggtaattt ggacatacgc
     5701 gccttcacag cccgcatcgg ctgggtacct tccgatacgc tgggcgtgta tcgcatcgag
     5761 tacgacacca ccggctttac gctcggcaca ggcaatgtca agttggcgcc gggccgaatg
     5821 accacactca ccggccttag ggaaaataca gattatcagt actatatcac tgtattgtgc
     5881 gccaacggcg acaccagcac taccgtgggg ccatttacct tcagaacgct gtgggccaac
     5941 aacgtaggcg tcacagaatt gatctcgccc atttccggtt gtgcgctttc ggccaccgaa
     6001 gaaatgcgtg ttcgcatccg caatttcggc ggtaatccgc aatcgcttat tcccatcaaa
     6061 ttcagcgtca atggtcaggt agcgcccgtc aacattccat tggatggctt ctacaccggc
     6121 gttttgggaa aagacagtac tgacacgttc ctgtacgatc tcacctggga cttttccgaa
     6181 cccggcgagt acatcatccg catttggacc gatttgcagt cggacagcct acgcgccaac
     6241 gacacactga ccgttgtcat caacagcctg ccggtgatag acaacctgcc ctatctcgaa
     6301 gattttgaaa cctggaacgg cggctggcgc ccaagtgtgg ccggcaccaa ttccagttgg
     6361 gaattcggcg ttcctgccgg cgctctgctc aatgcggcag cttcgggcca gaacgcctgg
     6421 gtgacgaatt tacaaggcga gtacaacaac agcgagttgt cctatctgga atcgccgtgt
     6481 attgacttct ccgggcttac ccaagacccg cgtctgctct tttcgctctg gtttgattca
     6541 gaagcctgct gcgacgaggc atgggtagag cagagcatca acggcggtca aacctggacc
     6601 aaagtgggcg cctccggtac gggcctcaac tggtacaatg acaacctgaa tcaatggtgg
     6661 aacggcacgg gcggattcaa tggctgggtc actgcattca acacactgac cggcacagcg
     6721 ggtcagtcgg atgtgcgcat tcgtttcgta ttctcttccg atggttcggt ggtgcgcgaa
     6781 ggcatgggcg ttgacaatat cttcatttcg cagcccgtgg ccagagacct cctcaatcaa
     6841 ggcgctacct acactgccgc agccgcttgc ggaacagaaa caggcactgt acgcatcaat
     6901 gtcgtcaacc tcggcaccca ggctcaaacc ggcttcaacg tagcgtaccg catcaatggc
     6961 ggcgctccgg tagtggaaaa tgtaggcgca ctcaacatcc cgcccaatca gcaggctgct
     7021 tataccttca ccacgccgct caattttacc caaaacggaa catacaatat tcaattatgg
     7081 acagagcttg cgggcgatct gcaactcagc aatgatacct cttcgctcat cgtcaatgtg
     7141 gaattagtgc ctgtcattgt aggcctgccc tactttacca actacgaaaa cggccgggac
     7201 ggctggcgcg taggcatcgg cagcgctgct tcatcctggc aattcgggcc acccgcagcc
     7261 cctatgctca atgctgctgc atcgggccag aatgcctggg taaccaacct ggccggccag
     7321 tacaacaaca atgagctgtc ttatctcgta tcgccttgct acgacttctc cagcgcctga
     7381 gcttttcgat gtggttcgac tcagaagcct gctgtgatga ggcttgggta gaacgcagta
     7441 ttgacggcgg ccaaacctgg accaaagtgg gcgcatccgg caccggcctc aactggtaca
     7501 atgatgcagg cagtcaatgg tgggacggca caggcggctt caacggctgg gtaacggcct
     7561 tcaacaccct cgccggcgtg gcgggccagg ccaatgtgcg cattcgcttt gtgttctcca
     7621 cggatggttc tgtaacgcgc gaaggcatgg gtgtggatga tgtgcgcatc tccattcctg
     7681 ccgcggcaga tatttccaat gcagtaacag catacacggc cgcctcttcc tgcggcgttg
     7741 aaaacggtac ggttcgctta accatcgtca acttcggtac acaggcgcaa accggtttca
     7801 acgtggcgta cagcgtcaat ggtggtacgc cggtggtgga gaacgtaggc gcgctcaatg
     7861 tgccggccaa ccagcaggct acctatacat tcactacgcc ggtgcaattc actcaaaacg
     7921 gcagctacaa catcaccgca tggacggaac tggcaggcga tgaagccctg agcaatgaca
     7981 caacaggcat caccgtcaat gtggaattgg tgcccgttat tcccgacttc ccttatacca
     8041 ataactttga gagcggcaat ggcggctgga aagtcggagc cggcagcaac tcatcttcct
     8101 ggcagtttgg tcaacccgta ggcgccctcc tcaactccgc ggcaagcggc atcaatgcct
     8161 gggtaaccaa tctcgcagga gcatacaata ccaatgaatt atcttactta gtctctcctt
     8221 gcttcannnn nnnnnnnnnn ngcatggatc cgttcctgag tttctccctc tggttcgact
     8281 ctgaagcctg ctgtgatgag gcctgggtag aacgcagtat tgacggcggc caaacttggg
     8341 ccaaagtggg tacttcgggt accggccaaa actggtacaa tgatgatgga aaccaatggt
     8401 gggacggcac cggcggcttc actggctggg taacggcata caacaccctc accggcgtgg
     8461 ctggtcagtc caatgtccgc atccgatttg tattctcttc cgacggctcg attcagggag
     8521 aaggtatcgg tgtggacgac atcaatattg acgctacgcc gacggccctt gtggagccga
     8581 ttgatcgcct cacgggcatc agcctggcgc ccaaccccac gggcggggtt tctatattgt
     8641 cggtatcgtt cgccgagcct gtggatgcca ttatcagcat acacgatatg ctgggccgtc
     8701 aggtatcggt catcaaagac cagcgcgttt ccaacggggc ttatccaatt gacctgagta
     8761 accaacctgc gggcatttat ttggtgcgcg tgatggtgga cggcaaagtg aacacgctga
     8821 aattagtacg cggccaataa ctcgaattag taaataaaac tgtcgcaagg gcgtctctgt
     8881 cattagagcg ccattgtgtt tttaaacctt ttcggtttaa aaactgtatc gaaggcaaga
     8941 tcaactcgca ccatgaaaat aggcattgtt tgttatccca cttacggcgg cagcggcgtc
     9001 atggccaccg aactggggct gggcctggcc cgccacgggc atcaggtaca cttcatcact
     9061 tcccggcgcc cgccgagact cagcacgttt cacgaaaacg tgtattttca cgaagtttca
     9121 ggcgaagatt atccactgtt tgaatatccg ccttacgaca cggccttagc cagcaaaatg
     9181 gtggacgtgg tgaagtttga aaacttagac ttgctgcacg ttcattatgc catccctcat
     9241 gctaccgtgg cttatattgc caaaaaaatt ctcctcacac aggggcgcta tgttccggtg
     9301 gtgactaccc tgcacggcac cgacattaca ttagtgggca gtaacaaagc ctttgctcct
     9361 gtggtggaat tttccattaa caaatcggac ggcgttacgg cagtttccga aagcctcaaa
     9421 caagaaacct gcgaatactt tgacattcag cgagatatcc gggtaattta caatttcatt
     9481 gattttgcca ggttttccaa atccgacaaa gaccatttca aaaaagccat tgcccccaac
     9541 ggcgagcgca tcattgtaca cacttccgaa aacgcaaacg cgtagaggat gtcatcgaag
     9601 tgtttcggcg catacacgag cataccccgt ccaagctctt gctcatcggc gacggcccgg
     9661 aacgctacca tttggaggag ctttgccgcc gcatggacct ctgccatgag gtgcgttttt
     9721 tgggaaaaca ggatgccata gaggaattgc tggcagtgtc cgatctcttc gtcatgccct
     9781 ccgaaagcga gagcttcggc ctggcagcat tagaggcgat ggcctgcgaa gtgcctgtca
     9841 tttcttccaa cgccggcggc ctcccggagg taaacattca cggccaaacc ggctatctca
     9901 gcgaagtggg cgatgtggaa agtatggcgc gcaatgcgct acaaatattg aacgacgacg
     9961 acaccctgcg ccgcttccgc gatgccgctc tggagcaagc ccgccgcttc gacatcagca
    10021 tcatactgcc gcagtacgag cagtattatg aggaagtact ggcagcctcc gcgttgcggg
    10081 tagaataaaa tggaattgtt tcataacgaa aaagcccgcc gaacaacatt cgacgggctt
    10141 tttttgcctc ctgactttgc agcagcgatt atccaccgaa gtcgtcaaag gagatattct
    10201 cttgcggaac acccaattcg tccaatgttt tgatgaccga ctggttcatc atcggcgggc
    10261 cgcagaagta gtattccact tcttccggtt ccgggtgatc tttcagatac ttttcataca
    10321 acaccggcat aatgaagcct acaaagccct cgccttctgt atcgtggatg tcttttttgg
    10381 ctacccagtt gtcttccggc agtggattgt ccaatgcgat gaagaactta aaattgggga
    10441 actcccgctc aatagcccgg aagtcttcca cataaaacaa ctcccggcgg gtacgacccc
    10501 cgtaccagaa agatacttta cggtcggtgg tttttagcgt atggaacaag tggaaaaggt
    10561 gcgaacgcaa aggcgccata ccagcaccgc cgccgatgta aaccatttcc ttcttggtcg
    10621 gcttgatgaa gaactcgccg taaggccccg aaactaccac tttatcgccc ggcttgcggg
    10681 tgaacacata agaagagcat atcccaggat tgacattcat ccagccgttg gtagcacgat
    10741 caaaaggcgg cgtagccacg cggatgttga gcatcacgat gttgccctcg gcggggtggt
    10801 tggccataga gtaagcgcgg aaaatcggct cctcattttt cattttgaga ccccacaatt
    10861 tgaacttgtc ccactcgggg cggaactctt ccggagcctt gcccatgcgc gggtgcgaag
    10921 taatgtcaat ggtagagtag tcgcactcaa tggnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnct cgaagtgcag gtgttcgcct tccggcaatt tcaccacaaa ctctttgata
    11041 aaagtagcca cattgtagtt ggaaaccact tcgcactccc acttcttgat gccgaaaatc
    11101 tcttccggca cgcgaatgcg catgtcttgg cgaactttga cctgacaggc aagacgcaga
    11161 ttttcagcca cgtctttgcg cgtgaagtgg ttcatttcag tgggcaatac atcgccgccg
    11221 ccggcatcca catggcattt gcacatggca caggtaccgc cgccgccaca ggcagagcca
    11281 cagcccgccc aaggtgatca gcgcgaggct cgccgccggc atcggtggca gcagcacgac
    11341 agcaccgggc cagcccgcca ccacgccagc aatccagacg acgaccgata ctccccaggc
    11401 cagcgcctgc agcagcggtc cgtcggcgcc cagcggcatc agcagcatcg ccggcaccgc
    11461 gcagggcatg atccagaagc tcgtcaccgg caccgcgatc aggttggcaa cgatgccgta
    11521 cagctgaaac cggtcgaagt gataggcggc gaacggcgtc gtggccaggc tcgccaccnn
    11581 nnnnnnnnag gatgatcccg gcaggataga gcaggattcg gtacaccgtt cgccgctcac
    11641 ccggccggcg ctcgcgtcgc gcgcgcaccg cttcgtaggt ggcgaccagc gccaccaccg
    11701 cggcgaagct catctggaag ctgggcccgg tcaacgagtc cggctgggtg agcaacacgg
    11761 ccagcgccgc ccaggcgatc agccgcatgg aaatgccctg ccggtcgacc agcaccgcca
    11821 gcaacgcaat cgccaccatc aggaacgagc gctgcgccgg caccggcgcg ccggcgagca
    11881 gcgtgtagaa cgcggcggcg atcaccgaca ggatcgccgc cagcttcttt gttggccagc
    11941 gcaatgccaa ccacggaatc agcgccagaa gcgaacgcgc ggtgacgaag acgatgccgg
    12001 cgacgaggcc gatatgcagg ccggagatcg acagtaggtg ggccaggccc gaggcgcgga
    12061 tcgcgtccat cgtcgccggc ggaatcccct
//
LOCUS       DAORLF010000090        12077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2820_length_12077_cov_2.028864, whole genome shotgun sequence.
ACCESSION   DAORLF010000090 DAORLF010000000
VERSION     DAORLF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_12077_cov_2.028864"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..788)
                     /gene="arr"
                     /locus_tag="PK198_09285"
     CDS             complement(339..788)
                     /gene="arr"
                     /locus_tag="PK198_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HRF38972.1"
                     /translation="MKNDNKNELKPAAGGATPFAQTYFHGTKADLKIGDFVETGFNSN
                     YGQQKNAKYIFLSATLDAAIWGAELATGDGRERIYLVEPTGAVEDDPDLTDKKFPGNP
                     TKSYRSTEPFRVVGEVSAWQGHPAEQVRAMKDALERLKGQGVNSLND"
     gene            1129..2238
                     /locus_tag="PK198_09290"
     CDS             1129..2238
                     /locus_tag="PK198_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HRF38973.1"
                     /translation="MNTDVFFNISKIIERDSKTTTYKFALLRGVIDIIQDNSPFISRS
                     DDRVYFPTGLLIDKWLLYYYPILESLTVIPQINGQAQLAFESQFKKIIDAYSETGGFS
                     AFNNDLRNKGIPQKVHSIFFDLSKKLHHTITKMPMKYIGRSISDDYYSIFNFENDKRK
                     GGKIVDIEYLINNFGSFSIPVEYYEAFQILGSFINGQDSIFFKWAEFSVNASGKNISI
                     EKVLTEVLKSPITERELQESKKIYKSILNREGKVFCVWTGKSITSYDVDHLIPFSVWK
                     NNDLWNLLPSQASINNKKRDKIPSVELIEEQKDLILHFWELINESQPQRFQKEIQVAL
                     LGKKTFHDWQKTAIKQLQQSCEYLISNRGYEEWKI"
     gene            2226..3092
                     /locus_tag="PK198_09295"
     CDS             2226..3092
                     /locus_tag="PK198_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRF38974.1"
                     /translation="MENLKSNPNSHLTAKERDSLSFPAKFLLQNNFLVGDILDFGCGF
                     GKDVEILKSKKINITGYDKYYFPEYPTSTFDTIMCLYVLNVLLPEEQAMVLMELSRLV
                     KPSGKVFIAVRRDLQFEGFRTHKLHQKKTYQCVVNLNSKSLFKNDFCEIYEYCHFNQL
                     QRKENPTCPFCNPDSGRELIVESATAYSIFDKFPVSNGHALIIPKRHCEDYFNLTFKE
                     QSACIFMLNTVKQIIQKTFNPDGFNVGINIKEAAGQTIPHVHVHLIPRYEGDVEHPRG
                     GIRGVIPNRKDY"
     gene            complement(3132..>4023)
                     /locus_tag="PK198_09300"
     CDS             complement(3132..>4023)
                     /locus_tag="PK198_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRF38975.1"
                     /translation="TEFEELVNQVVFVSATPADYELEQTGGLIVEQLIRPTGLLDPPI
                     EVRPSINQMDDLLEEADERIKKGERVLVTTLTKRMAEELTKYLSKLGIKVRYVHSEVD
                     TMERVEILRDLRLGEFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRNERSLTQTA
                     GRAARNVNGLVIFYADHITDSMQKTIDETNRRRAIQMAYNEANGIVPRTVSKSVEDIM
                     GQKSILDVRGPKAAKAYIEPEQPSIAADPLISYLSRDQIENLILETEKKMKQAAKDLD
                     FVTAAMHRDEIFALKGRLRD"
     assembly_gap    4024..4038
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4039..5166)
                     /locus_tag="PK198_09305"
     CDS             complement(<4039..5166)
                     /locus_tag="PK198_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRF38976.1"
                     /translation="MDFKLTSPFVPTGDQPQAIEQLVAGIQAGERAQVLLGVTGSGKT
                     FTMANVIAQLNRPTLVLTHNKTLTAQLYGEFREFFPENAVEYFVSYYDYYQPEAYLSV
                     TDTFIEKDLLINEEVDKLRLRATSSLLSGRRDIILVASVSCIYGMGNPEDYKTGIIRI
                     HKGQVISRNSFLYQLVDSLYSRTETDFKRATFRVRGDTVDINLPYVDYGYRVTFFGDE
                     IESIERLEVDSGKRIEGMDNAAIFPANLYIAPRERLHQIIKSIEDELYEQEKYFERER
                     KYMEAKRIRERTNFDLEMIKELGYCSGVENYSRFFDGRKEGTRPFCLLDYFPDDYLMV
                     IDESHVTLPQVRGMYGGDRSRKLNLVNFGFRLPSAMDNRPLN"
     gene            complement(5215..>6298)
                     /locus_tag="PK198_09310"
     CDS             complement(5215..>6298)
                     /locus_tag="PK198_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRF38977.1"
                     /translation="AIIIGRVNCDEFAMGSSNENSCYGPTRNAADPERVPGGSSGASA
                     VAVQAGTCLAALGSDTGGSVRQPAAFCGVTGFKPSYGRISRHGLIAYGSSLDQIGTLA
                     HSVEDTALLLEIMAGPDAYDSTASVEPVPAYSRLAPPDRPMRVAYFRTAIEHPHLDEG
                     IRALTLRFADRIRQAGHTVEAIDFEYLDYIIPTYYVLATAEASSNLSRYDGVRFGHRS
                     QAAHDLNSTYKRSRTEGFGAEVKRRIMLGTFVLSSGYYDAYYTKAQQVRRLIHDRTKE
                     IFQQYDFILMPAAPTTAWRLGEKISDPVEMYLADVFTVQANLAGIPAIALPIGQHPED
                     GLPVGVQLMADAFQEEALLGFAHSLF"
     assembly_gap    6299..6368
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6369..6621)
                     /locus_tag="PK198_09315"
     CDS             complement(<6369..6621)
                     /locus_tag="PK198_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRF38978.1"
                     /translation="MPAFRTLAEVQAALRSGALTMESLVASYLERIEATRHLNIYVEV
                     FEQEALEQARRQDAAKPAGRLLGMVLAIKDVICYKDHEVT"
     gene            complement(6682..7854)
                     /locus_tag="PK198_09320"
     CDS             complement(6682..7854)
                     /locus_tag="PK198_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF38979.1"
                     /translation="MIYRIAHDQIIDDIQFFPALAIIGPRQVGKTTLARNLRIPSGKP
                     LLYLDLEWEEDRAKLSDAGAYLLQHEDKCVIIDEVQIMPSLFQILRSLVDRKREPARF
                     ILLGSASPELLRNSAESLAGRIAYHELCPFSLLEIWEENILRRHWFCGGFPNAFLAPS
                     EQVAGKWLQQFYTTYIERDITHVIGRDVNSAKMLRFVRMLAHIHGNILNLSELSNSMN
                     IATQTLSSYLDLLEGSFLIRRLEPFYVNVGKRLTKSPKFYFRDSGFYHAVARFRDMED
                     LYASPAIGASWEGYVIEEIFRIAGNHCEYYFYRTQNGAEIDLLLITPRSEKVCIEIKA
                     ANAPKISRGFHHSVADLQPEHQYVITPESERYQRSDGIVMIGLYHFLKYDLPLLIS"
     gene            8085..8660
                     /locus_tag="PK198_09325"
     CDS             8085..8660
                     /locus_tag="PK198_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRF38980.1"
                     /translation="MNDKDQLQEQEMAQPEMPDMEPNTASESMEKSDNEVIVEINEGN
                     YEALRAENAELKDKYLRIYAEFDTFRRRMMKEKLDLIKTAAQDTMTALLPVLDDFDRA
                     KKSAEANNSPEQFSEGVALVYQKLHNTLKNIGLETMETHQQPFDPEWHEAMAEIPAPA
                     DELKGKIIDTIEKGYVLGGKIIRHAKVVIGK"
     gene            8670..>9181
                     /locus_tag="PK198_09330"
     CDS             8670..>9181
                     /locus_tag="PK198_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRF38981.1"
                     /translation="MAKRDYYEVLGVDRNADADTIKKAYRKQALQYHPDRNPDNKEAE
                     NRFKEAAEAYEVLSDADKKAAYDRYGHAGVDQMSGGRPQGGMSYEDIMAHFGDIFGDA
                     GGGNPFAEFFGGGRGGGRTQQGQRGSNIRVKIALSLEEIATGVTKKIKVRKQTTCDTC
                     NGSGARDHNSV"
     assembly_gap    9182..9241
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9242..>9516
                     /locus_tag="PK198_09335"
     CDS             <9242..>9516
                     /locus_tag="PK198_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRF38982.1"
                     /translation="MQTTAACPSCNGSGKTITATCNKCRGEGTMEGEETIEIKIPGGV
                     EEGMQLTLRGKGNAGRRGGPAGDLLVTIEEKSHEILHRDGMNVVYEL"
     assembly_gap    9517..9642
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9643..9834
                     /locus_tag="PK198_09340"
     CDS             <9643..9834
                     /locus_tag="PK198_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535991.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRF38983.1"
                     /translation="KGLPSVQSYERGDQLIHVNIWTPKNLTDEERSILEKMREMKNFQ
                     PNPGKSERSFFDKMREMFS"
     assembly_gap    9899..10026
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10027..10400
                     /locus_tag="PK198_09345"
     CDS             <10027..10400
                     /locus_tag="PK198_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRF38984.1"
                     /translation="LRFEAEIPDTSTVYDLWLSLDHSPDFEWQNLYVQVHTLFPDGRR
                     LSSPLSLELADQGGVWQSDCNSKRCRFRVPIQEGAWFQQTGKYVFTIEQYMRVNPLPG
                     VQSVAFQIVDTGKTKEEAMKK"
     assembly_gap    10443..10483
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10540..>11030
                     /locus_tag="PK198_09350"
     CDS             10540..>11030
                     /locus_tag="PK198_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRF38985.1"
                     /translation="MIVCTSCRRSMPADARFCPYCGAARPVELRYNIHPEGEHADQEI
                     AALFFPALKDRLRDLLGVSVDNQPFLERVYSSGFRDMVQRRAAQTAQSIKHQPRSNDQ
                     LNHLIENLLEDLLDFFLVRHCADLLPFPLPEDILSWQMAAPARDDMQEMILDYLDLAS
                     EELP"
     assembly_gap    11031..11050
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11051..11386
                     /locus_tag="PK198_09355"
     CDS             <11051..11386
                     /locus_tag="PK198_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38986.1"
                     /translation="LPTHTDMLKMPVEQLRNAAKYFLFADRDERIFLIGDLSVLGSGS
                     EGFALTENALYWKAPLHTARRIMYPELESIRMEPDWMLVNGHFFNAGKTLNVKLFLLL
                     KKLGRMGGM"
     gene            11400..>11603
                     /locus_tag="PK198_09360"
     CDS             11400..>11603
                     /locus_tag="PK198_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38987.1"
                     /translation="MQVKNRKALYDEVMKKGQKLWTREELILAINLYCKTPFGKLHSR
                     NPDVIQLSKLLGRTPSSIAYKLVN"
     assembly_gap    11604..11622
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11674..>12077
                     /locus_tag="PK198_09365"
     CDS             11674..>12077
                     /locus_tag="PK198_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRF38988.1"
                     /translation="MDKSVWNEFVNNWDALSYESEILLAKHENQTIEEIYYTPELDHL
                     KEGRVREQIVKVRVNQSFFRKTILASYSFTCCITGLQQPEFLVAGHIKPWSQDEKNRL
                     NPRNGILMNALHDKAFENGLITIDTNYKIRISS"
BASE COUNT         3108 a         2777 c         2889 g         2824 t
ORIGIN      
        1 ctgcatcata tcaaaccaat aaggataatc ggcagtatcg cggcctgtgg aaaaagatgg
       61 ttgtgagaag agaaaaagag gccctgctgt cagggcaacg accgtcgcta tttttcggat
      121 gtccatgtta tcaagcctgt ttcgtgtgat gaagtaagga gaacacatct gcaaaagtat
      181 aaatcaacgt gccggagcgg caaaatcgaa gggggacaaa aagggggaca aatttttcat
      241 cgcgccatta tgagccaata gcgacgaccc cgccgtagct gacagcttgc ggttttgggg
      301 atggtcttaa atagttgtct tttttaaatt aaagcttgtc agtcgttcaa ggaattaacc
      361 ccttgccctt tgagccgttc caaggcgtct ttcatagccc tcacctgctc ggcagggtgt
      421 ccttgccagg cggaaacctc tcctaccact ctgaaaggct ctgtggaacg atacgatttt
      481 gttggatttc ccggaaattt tttgtctgtc agatcaggat cgtcttcaac cgctccggtg
      541 ggttctacca gataaatcct ctcgcgcccg tcgcccgttg caagttcggc gccccaaata
      601 gccgcgtcca gggttgcgga cagaaagatg tatttggcgt ttttctgttg cccatagttg
      661 gagttgaaac cggtttcaac gaagtcgccg attttcagat cagccttagt tccgtggaaa
      721 taagtctgag cgaaaggcgt cgcgccgccc gccgccggtt taagctcgtt tttgttgtcg
      781 tttttcatga agcagtgtgc atttttgttg gtgttggagg gagggtggcg tcatcatcgt
      841 tccggttcat taagcgacat ttcgcttttt gctcaactga tttgttcgtt tgttccgtag
      901 tagctacctt tgtcatagat caaagttgca cgattgactt gcgatttcaa aagtacaact
      961 ttagacctac ttttccctaa ttgacgcaac aatgtccaca tcaactatca ttacagaagt
     1021 tattatgctc gcaacaaccc gccctgtttt ttctggccat gagacttttc agtgccggca
     1081 tttgtggctg aaaaaaggtt atgaaagccc taaaaaactg ccaaaaaaat gaacaccgac
     1141 gtttttttca atattagcaa aatcattgag cgagatagca aaactacaac gtacaaattt
     1201 gctctcctac ggggagtgat tgatattata caggataatt ccccttttat atctcgttcc
     1261 gatgacaggg tttatttccc tactggattg ctgattgaca agtggctact ttactattat
     1321 cctattttgg agtccttaac agttatccct caaataaatg gccaagcaca acttgctttt
     1381 gaatctcagt tcaagaaaat cattgatgcc tatagtgaga ctggaggatt ctccgctttc
     1441 aataacgacc tgagaaataa aggaatccct caaaaagtac actcgatttt ttttgatcta
     1501 tcgaaaaaat tgcatcacac gattacgaag atgccaatga aatatattgg caggtcgatc
     1561 agtgacgatt attattcaat atttaacttt gaaaatgaca aaaggaaagg tggaaaaatt
     1621 gttgatattg aatacctgat aaacaatttt ggttctttct caatacctgt ggaatactat
     1681 gaagcatttc agatactcgg cagtttcatt aatggccaag attcgatatt tttcaaatgg
     1741 gcagagtttt cagtaaatgc ttcaggaaag aatatatcaa ttgaaaaagt tctaactgaa
     1801 gtattgaaaa gccccataac tgaaagagaa cttcaggaat caaaaaagat atacaaatca
     1861 attttaaata gagagggcaa agtattttgc gtttggactg gaaagtctat caccagttac
     1921 gatgttgatc acttgatccc gttttcagtt tggaaaaaca atgatttatg gaatttacta
     1981 ccctcacagg ccagcataaa caacaagaaa agggacaaga ttccttctgt agaattgatt
     2041 gaagaacaaa aggatttaat tcttcacttc tgggaattga taaatgaaag ccagcctcag
     2101 agattccaaa aggaaattca agttgcgcta ttgggaaaaa aaacattcca tgactggcag
     2161 aaaaccgcga ttaagcaact tcaacaaagt tgtgagtatt tgatctcaaa tagaggttac
     2221 gaagaatgga aaatttgaaa tctaacccga acagccatct aacagctaag gaaagagact
     2281 ccctctcttt tcccgctaag tttttattgc aaaataattt tttagttggc gatattttgg
     2341 atttcggctg tggcttcggt aaagatgttg agatactaaa gtcaaaaaaa atcaacatca
     2401 ctggatacga taaatactat ttcccggaat acccaacaag cacgtttgat accattatgt
     2461 gtttgtatgt acttaatgtt ttgctcccgg aagaacaggc aatggtactc atggaacttt
     2521 ctcggctggt caaaccaagc ggaaaagttt ttattgcggt tagacgagat ttacagtttg
     2581 aaggtttcag aactcataaa ctacatcaga agaaaactta tcagtgtgtg gttaacttaa
     2641 attcaaagtc tcttttcaaa aatgattttt gtgaaattta tgaatactgt catttcaatc
     2701 aactgcaacg aaaggaaaat ccaacgtgcc cattttgtaa tccagactca ggaagagaac
     2761 tgatcgttga atctgctact gcatattcaa tattcgataa atttcctgta agtaatggcc
     2821 atgccttgat tattcctaaa agacattgcg aagattattt caatctaact ttcaaagagc
     2881 agtcagcatg tatcttcatg ttaaacacag ttaagcaaat catacagaaa acgtttaatc
     2941 ctgatggatt taatgttggt attaatatca aagaagcggc aggacaaacg atccctcatg
     3001 ttcatgttca tttgatccca cgatatgaag gtgatgttga acacccaaga ggtggaataa
     3061 gaggtgtcat tccaaataga aaggactact agtttctgaa atatctactt gatagcgatt
     3121 gtccccccct ctcaatccct caacctcccc ttcagtgcaa aaatctcatc gcggtgcatg
     3181 gcggccgtta cgaaatcgag gtctttggcg gcctgtttca ttttcttttc cgtttccaga
     3241 atgaggtttt cgatctgatc gcggctcagg tagctgatga gcgggtcggc tgctatgctc
     3301 ggctgttccg gttcgatgta agccttggca gctttggggc cgcgtacatc gaggatggat
     3361 ttctggccca tgatgtcttc cacgcttttg ctcaccgtgc ggggcacaat gccgttggct
     3421 tcgttgtacg ccatttggat ggcccggcgg cgattggtct cgtctatggt tttttgcatc
     3481 gaatcggtga tgtggtcggc gtagaagatg accaagccgt tcacgttgcg ggcagcgcgg
     3541 ccggccgtct gggtcagcga gcgctcattg cgcaggaagc cctccttgtc tgcatcgagg
     3601 atggccacca gcgatacttc gggcaggtcg aggccctcgc gcaggaggtt cacgcccacc
     3661 aagacatcga actcgccgag ccgcaggtcg cgcaggattt ccacacgctc catcgtgtcc
     3721 acttcggagt gaacatagcg cactttgatg ccgagtttcg acaggtattt ggtcagttct
     3781 tcggccatgc gcttggtgag ggtcgttacc agcacgcgct cgcctttttt gatgcgctcg
     3841 tcggcctcct ccagcaggtc gtccatctga ttgatgctgg gccgcacttc gatgggcggg
     3901 tctaaaaggc cggtgggccg gatgagttgc tccacgatga gcccgccggt ttgttccagt
     3961 tcgtaatcgg cgggcgtggc gctgacaaac acgacctgat tgaccaattc ctcaaactcc
     4021 gtannnnnnn nnnnnnnnat tgagcggtcg gttgtccatg gcgctgggta gccggaagcc
     4081 gaagttgacc aaattgagct tgcgcgagcg gtcgccgccg tacatgccgc gcacctgcgg
     4141 gagggtgacg tggctctcgt caatgaccat caggtagtcg tcggggaagt aatcgagcag
     4201 gcaaaacggg cgggtgcctt cctttctgcc gtcgaaaaag cgcgagtaat tttccacccc
     4261 gctgcaatag cccaattctt tgatcatctc caggtcgaaa ttggtgcgct cgcgtatgcg
     4321 tttggcttcc atatatttgc gctcccgttc aaagtacttt tcttgttcgt acagttcgtc
     4381 ctctatggat ttgatgatct gatgcaggcg ctcgcggggc gcaatgtaga ggttggccgg
     4441 aaagatggcc gcgttgtcca tgccctcaat gcgtttgccg ctgtccacct ccaaacgttc
     4501 gatgctttcg atctcatcgc cgaaaaaagt gacgcggtag ccgtagtcca cataaggcag
     4561 gttgatgtcc accgtatcgc cgcgcacgcg gaaagtggcg cgtttgaagt cggtttcggt
     4621 gcgggaatag aggctgtcca ccaattggta caaaaaggaa ttgcgggaaa taacctgccc
     4681 tttgtgaatg cgaatgatgc cggttttgta atcctccggg ttgcccatac cgtaaatgca
     4741 ggacaccgag gccaccaaaa tgatgtcgcg gcgcccggac agcagcgagg aggtggcgcg
     4801 caggcggagc ttgtccactt cttcgttgat gagcaggtct ttttcaataa aagtatcggt
     4861 gacggatagg taggcctccg gttggtaata atcgtaataa gagacgaaat actccaccgc
     4921 attttcggga aagaactccc gaaactcgcc gtacagttgg gcggtcaggg ttttgttgtg
     4981 cgtcaacacc agcgtgggcc ggttgagttg cgcaatgaca ttggccatcg tgaaggtctt
     5041 gcccgaaccc gtgacgccga gcaaaacctg cgcgcgctcg ccggcctgta tgcctgccac
     5101 caattgttcg atggcctgcg gttggtcgcc ggtgggcaca aaaggcgatg tgagtttgaa
     5161 gtccatttat tcaatgtgct gatgtggcaa tgtgccgatg tgctgaaaag aaccctaaaa
     5221 caaactatga gcaaatccca gcagggcctc ctcctgaaag gcatcggcca tgagctgcac
     5281 gcctacgggc agcccgtctt ccgggtgttg tccgatgggc agggcgatgg cgggaatccc
     5341 ggccaagttg gcctgcacgg taaacacatc ggcgaggtac atctctaccg ggtcggaaat
     5401 tttctcgccc aagcgccagg ccgtagtggg cgcagccggc atgagaatga aatcgtattg
     5461 ctgaaaaatt tctttggtgc ggtcgtgaat gaggcggcgc acctgttggg ctttggtata
     5521 ataagcgtcg taatagccgg aactgagcac aaaggttccc agcatgatgc ggcgtttcac
     5581 ctcggcgccg aagccctccg tgcgcgagcg tttgtaggtg ctgttcagat cgtgggccgc
     5641 ctgactgcga tgcccgaatc gcacgccgtc gtaccggctc aggttggacg atgcctcggc
     5701 tgtggccaat acataatacg tcgggatgat gtagtcaagg tattcaaaat caatggcttc
     5761 cactgtgtgt ccagcctgtc tgatccggtc ggcaaagcgc agggtcaaag cccggatgcc
     5821 ttcgtccaaa tgcgggtgtt ctatggcggt gcggaaatac gccacgcgca tgggccggtc
     5881 gggcggagcg aggcgggagt aagccggcac gggttccacc gaggccgtac tgtcgtaagc
     5941 atcggggccg gccatgattt cgagcaagag cgccgtgtct tccacactgt gggccagagt
     6001 gccgatctga tcgagcgaag aaccatacgc gatgagtcca tgccgggaaa tgcgcccgta
     6061 gctgggtttg aacccggtga cgccgcaaaa cgctgccggt tgccgcaccg atccgcccgt
     6121 atcgctgccc agggccgcca gacaagtacc cgcctgcacc gccactgccg aggcgcccga
     6181 tgaaccgccc ggcacgcgtt cggggtcggc agcattgcgc gtggggccgt agcaggagtt
     6241 ttcgttgctg gaacccatcg caaattcatc gcagttgacc cgcccgatga tgatggcgnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnncg gtcacctcgt ggtctttgta acaaatcacg tctttgatgg ccaacaccat
     6421 acccaatagc ctcccggcgg gcttggcagc gtcttgtcgg cgggcctgct ccagcgcttc
     6481 ctgctcaaaa acctctacat aaatattgag atggcgggta gcttcaatgc gttccagata
     6541 ggatgcgacc aatgattcca tggtgagcgc accggagcgc agtgcggcct gaacctcggc
     6601 caatgttcta aaagcaggca tgtcggcggg tgattgttga tggggcaaag gtaaggaaaa
     6661 tgcagatgct ttatatgtcg gctaagatat aagaagaggt aaatcatatt tcaaaaaatg
     6721 gtataaaccg atcatcacaa taccatcgga gcgttggtat ctctcacttt caggggtaat
     6781 gacgtattgg tgttcaggct gtaaatcagc tacgctgtgg tgaaatccgc gggagatttt
     6841 gggtgcgttg gccgctttta tttcgataca caccttctca gaacgcgggg taatgagcag
     6901 tagatctatt tctgcaccat tctgcgttct gtaaaagtaa tattcacaat ggttgcccgc
     6961 tatccgaaaa atttcctcga tcacatatcc ctcccaagat gcgccaatgg ccgggctggc
     7021 atataaatcc tccatatcgc gaaaacgcgc cacggcatga tagaaaccag aatcccgaaa
     7081 ataaaatttg ggcgacttcg tgagcctttt gcctacgttg acataaaaag gctccaagcg
     7141 tcgaatcagg aaagaacctt ccaaaaggtc taaataggaa gaaagggttt gtgtcgcgat
     7201 attcatagag ttgctcagtt cagacagatt cagaatgttg ccgtgaatat gcgccagcat
     7261 acgcacaaaa cggagcattt tggcactgtt cacgtcccgc ccgataacat gagtgatatc
     7321 tcgttctatg taggttgtgt aaaactgttg taaccatttt ccggctactt gctcgctggg
     7381 ggcaagaaaa gcattcggaa agccgccgca aaaccagtgc cgcctgagaa tattttcttc
     7441 ccaaatctcg agcaaagaaa aagggcatag ttcatggtat gcaatacgcc cggccaatga
     7501 ttcagcagaa tttctcagta actccgggga ggctgagcct aaaagaatga atcttgccgg
     7561 ttctcttttt cgatctacca acgatcgtaa aatctgaaac aaagaaggca tgatctgtac
     7621 ttcgtcaatg atgacgcatt tgtcttcgtg ttgcaacaga taagcccccg catccgacag
     7681 cttggcgcgg tcttcctccc attccagatc gagatataac aagggcttcc ctgacggaat
     7741 acgaaggtta cgtgcaagag ttgtctttcc tacttgcctc ggaccaatga tggccaatgc
     7801 ggggaaaaac tgaatatcgt ctataatttg gtcgtgagca atacggtaaa tcatgccgct
     7861 aagatatatt taaccaagca aataacaaaa tacatttatc aatttacttg gataaaaaat
     7921 gcaaaccaaa taaaacctac gctttcagag ttctggcagc gtcaataaac gctcctggct
     7981 acaacttgcc atgccaactg acaaaatggc ttgagtttcc tttcggcaat attattgcta
     8041 tatcttcgca gcgttacaaa acagaagttt taacaaagtt gacaatgaac gataaagacc
     8101 aattgcaaga gcaggaaatg gcgcaacctg aaatgcccga tatggagccg aatacagctt
     8161 ctgaaagtat ggaaaaatct gataatgaag ttattgtgga aataaatgaa ggcaattacg
     8221 aggcgttgag agcagaaaat gcagagttga aagacaagta tcttcgcatt tatgctgagt
     8281 tcgatacctt ccgccggcgc atgatgaagg aaaaattgga cctgataaaa acagctgccc
     8341 aggacactat gacagcgctg ctgccggttt tggacgattt tgacagggcc aaaaagagcg
     8401 ccgaagccaa taacagtccc gaacaattca gcgaaggcgt ggcattggtt tatcaaaaac
     8461 tccacaacac gctcaaaaat ataggcttgg aaaccatgga aacccatcag cagcctttcg
     8521 atcccgaatg gcacgaagcg atggccgaaa tccctgcccc cgctgacgaa ctgaaaggca
     8581 aaatcataga taccattgaa aaaggctatg tgctgggcgg caaaatcatc cgccacgcca
     8641 aagtcgtcat cggtaaataa ttcattatca tggccaaaag agattattac gaagtgctcg
     8701 gcgtggaccg caatgccgat gccgacacca taaaaaaagc ctaccgcaag caggccctcc
     8761 aataccaccc ggaccgcaat cctgacaaca aggaggcgga aaaccgattc aaagaggcag
     8821 ccgaagccta cgaggtgctg agcgatgccg acaaaaaagc tgcctacgac cggtatggcc
     8881 atgccggtgt ggatcagatg agcggcggca ggcctcaagg gggcatgtcg tatgaagata
     8941 ttatggcgca cttcggagac atctttggcg atgcgggcgg cggcaatccc tttgccgagt
     9001 ttttcggcgg cggacgcggc ggtgggcgca cgcaacaggg ccagcgcggt tccaatatcc
     9061 gggtcaaaat agcgctgtcg ctggaagaaa ttgccaccgg cgttaccaaa aaaattaagg
     9121 tcaggaaaca gactacctgc gatacctgca acggcagcgg cgcccgcgac cacaactccg
     9181 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 ngatgcaaac caccgcagcc tgcccttcct gcaacggatc gggcaaaacc atcaccgcca
     9301 cctgcaataa gtgccggggt gaaggcacca tggagggcga ggaaaccatc gaaatcaaaa
     9361 tacccggcgg cgtagaggaa ggtatgcaac tgaccctccg cggcaaaggc aatgccggaa
     9421 gacgcggcgg cccggcgggt gatctgctcg tgaccatcga ggaaaaatcg cacgaaatcc
     9481 tccaccgcga cggcatgaac gtggtgtacg aactgtnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagggatt gccctccgtg
     9661 caaagttacg aacgcggcga tcaactcatc cacgtcaaca tctggacgcc caagaacctg
     9721 accgacgagg agcgcagcat cctggaaaaa atgcgcgaaa tgaaaaattt ccagcccaat
     9781 ccgggcaagt cggagcgcag ctttttcgat aagatgcggg agatgtttag ttgacccaat
     9841 cgaacttcgc ggctcgaagc tgcctgattc atcccttata tcgaaacgaa gtcaatatnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnncact gcgttttgag gccgaaatac ccgacacttc cacggtgtat gatctgtggt
    10081 tgagcctcga tcactcgcct gattttgaat ggcaaaacct ctatgtgcag gtacacacgc
    10141 tcttccccga tggccgtcgc ctcagcagcc cgctctccct cgaactcgcc gatcagggcg
    10201 gcgtatggca atcggattgc aattctaaac gctgccggtt tcgggtgccc attcaggaag
    10261 gcgcctggtt tcagcaaacc ggcaaatatg tcttcaccat agagcaatac atgcgcgtca
    10321 atccgctgcc gggcgtgcaa agtgttgctt ttcagattgt tgatacagga aaaacgaagg
    10381 aagaggcaat gaagaagtga tggcggaaat tgattaaacc accatatctg accagttcgg
    10441 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgatttt gacaccttag
    10501 cagcaaaaac agcgccgata ccggccaccg ccgacactta tgattgtatg cacttcctgc
    10561 cgccgttcca tgcctgccga tgcgcggttt tgcccgtatt gcggggccgc ccggcctgtg
    10621 gaactgcgct acaacatcca ccccgaaggg gaacacgccg atcaggaaat tgccgccttg
    10681 ttttttccgg cactgaagga ccgcctccgc gacctgttgg gcgtatcggt tgataatcag
    10741 ccatttttgg agcgcgtgta cagttccggc ttccgcgata tggtgcagcg ccgggccgcg
    10801 caaactgccc aaagcatcaa gcatcaaccc cgctcgaacg accaactgaa ccatctcatc
    10861 gaaaacctgc tggaagacct gctcgacttt ttcttagtgc gccactgtgc cgatctgctc
    10921 ccgtttcctc tgcccgaaga catcctctcc tggcaaatgg ccgcccccgc ccgcgacgac
    10981 atgcaggaaa tgatcttgga ttatcttgac cttgcctccg aggaattgcc nnnnnnnnnn
    11041 nnnnnnnnnn ttgcccaccc ataccgacat gctcaaaatg ccggtagagc agttgcgcaa
    11101 tgccgccaaa tacttcctct ttgccgatcg cgacgagcgc attttcctca tcggcgacct
    11161 gagcgtgctg ggttccggca gcgagggttt tgccctcacc gaaaacgcgc tgtactggaa
    11221 agcgcccctg cataccgccc gccgcatcat gtaccccgaa ttggagagca tccgcatgga
    11281 acccgactgg atgctcgtca acggacactt tttcaacgcc ggcaaaacgc tgaatgtgaa
    11341 gctgtttttg ttgttgaaaa aattgggaag gatgggggga atgtgaattg ttgcaaaatt
    11401 tgcaagtaaa aaataggaaa gctctttatg atgaagttat gaaaaaagga caaaaacttt
    11461 ggacaagaga ggagttaatc ctcgccataa acttgtattg taaaacacct ttcgggaaat
    11521 tacatagtcg caatcctgat gtaatccaat tatcaaaatt attaggaaga acgcccagtt
    11581 caattgcata taaacttgtc aatnnnnnnn nnnnnnnnnn nngatatcag tctaaaggca
    11641 agaggtatta aaggagcaca aaatgtcagc aatttggata aaagcgtttg gaatgagttt
    11701 gttaataatt gggatgcttt atcttatgaa agcgaaatat tattagcaaa acatgaaaac
    11761 caaacgattg aagaaattta ttatactcca gaactcgatc atttaaaaga gggcagagta
    11821 agagagcaaa tagtaaaagt gagagtgaat cagtcttttt ttagaaaaac tattttggca
    11881 tcttacagtt ttacctgctg tataacgggg cttcaacaac ctgaatttct tgttgcagga
    11941 catataaaac cgtggagcca ggatgagaaa aaccgcctca atccaagaaa tggaatattg
    12001 atgaatgcgt tacacgataa agcatttgaa aatggattaa taacaataga tacgaattat
    12061 aaaatcagaa tttcttc
//
LOCUS       DAORLF010000091        12049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2824_length_12049_cov_1.971069, whole genome shotgun sequence.
ACCESSION   DAORLF010000091 DAORLF010000000
VERSION     DAORLF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_12049_cov_1.971069"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>324
                     /locus_tag="PK198_09370"
     CDS             <1..>324
                     /locus_tag="PK198_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF38989.1"
                     /translation="GVLAETLLYFGAAVLLMALLMGVFMYFMRQTIIVMSRLVEYDLR
                     KSLFEHYERLHTGFFKKNNTGDLMARVTEDVSKVRMYMGPAVMYGVNLISLFIMVIYS
                     MVAVS"
     assembly_gap    325..337
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <338..696
                     /locus_tag="PK198_09375"
     CDS             <338..696
                     /locus_tag="PK198_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF38990.1"
                     /translation="TLYCLAPLPILSVSIYYVSQIINKKSERIQQQLAVLNSTAQESF
                     SGIRVVKAYVQERAMGRFFSEQSEDFRQKSLSLGKVNALFYPLMLLLIGASTIITVYV
                     GGLQAVRGAITTGNIA"
     gene            715..1599
                     /locus_tag="PK198_09380"
     CDS             715..1599
                     /locus_tag="PK198_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF38991.1"
                     /translation="MLTWPVTAIGWIASIMQQAAASQKRIDEFLKTEPAIANPAHAPG
                     PLKGHIRFDQVNFVYPDTGVQALKNVSFELKPGQKMAIVGRTGSGKTSVADLLVRMYD
                     VSGGQVLIDGQDVRAHDLDNLRRRTGYVPQDGFLFSDSVSGNIAFGKPEAEQSEIERF
                     TKYAAIHDEIMGLPEGYGTVVGERGVTLSGGQKQRISIARALIKQPDILLLDDCLSAV
                     DTNTEKQILACFNEIMADKTAIVITHRIYSYLQFDKIIVLDGGRIVEEGTHDELLAAK
                     GYYYEMFERQAVEPEATL"
     gene            1756..1835
                     /locus_tag="PK198_09385"
     tRNA            1756..1835
                     /locus_tag="PK198_09385"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1790..1792,aa:His,seq:gtg)
     gene            1982..2683
                     /locus_tag="PK198_09390"
     CDS             1982..2683
                     /locus_tag="PK198_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF38992.1"
                     /translation="MLENIILLFQSTDIVREPEVVQQGSQSLFDIIRSGGPIGMTIVA
                     MQLGMSFLAFYIFIERFLTIKKASQIDESFMNNIRINVQSGNIQGAKALCQSTDSPVA
                     RMVEKGLMRIGKPLRDIDAAIENVGNLEIFKLEKNLSSLATIAGASPMIGFLGTVTGM
                     IIAFYQMASQQNVTPGVLAGGIYQALITTAFGLVVGIFALVAYNILVANVEKVVFKME
                     RSTTEFMDLLQEPTL"
     gene            2749..3147
                     /locus_tag="PK198_09395"
     CDS             2749..3147
                     /locus_tag="PK198_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF38993.1"
                     /translation="MGLKKRSGVTAEFSLASATDVIFLLLLFFMLTSTLVKIQPFDLP
                     QSDAKTVAPVTVVVTIRKDGSHTLNNNDIDPKAIERALGTALADAGSEAEATVTIVAE
                     TGTPFSHVVNVMEAASKNKARAIIATQPKS"
     gene            3167..3568
                     /locus_tag="PK198_09400"
     CDS             3167..3568
                     /locus_tag="PK198_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF38994.1"
                     /translation="MSMKKRARVTADFNMSSMTDIIFLLLIFFMLTSSVVAPNALNLK
                     LPGTSRAKVVKQKQVDDVRIRTSGAFALNGRSLDLGSLEQELARKASGSSEFTITISP
                     DDDTPTEHVVAVMDIALRYSINAILTTEKQR"
     gene            3680..4564
                     /locus_tag="PK198_09405"
     CDS             3680..4564
                     /locus_tag="PK198_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38995.1"
                     /translation="MAFDTLVSREEPKNRQRSFVISVSIHVVLLFLLFASFLTFPDPP
                     PGQEGILVNLGLPDVGQGDENAAAGTPDVAEPTPQPKEEAAPPKTEPAAKPEPVKEKE
                     VRVTEDPEAIALKRKQEQEKKAQAEADAKRKAEADAKLKAEAEAKRKADEKAAADKAA
                     ADKLKGELGGLLGSGKGKGNTGTPGNQGDPNGDPNADKLTGVSTGIGKVGGGLGGRKV
                     ASAPKITDDSQDRGTVVLRVCVDADGNVTSATFTQAGSTATSTKLKNLAIDNAKRWKF
                     GPGEDGQCGTITYNFTVQ"
     gene            4639..5265
                     /locus_tag="PK198_09410"
     CDS             4639..5265
                     /locus_tag="PK198_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF38996.1"
                     /translation="MQFAHKALGALRGQTFYRLMGSGRGEGFNPWPDWSVYALLQVWD
                     NEAEAAAFFAGSDLMGQYRRHTTQIWTIYMRPIMARGEWSGSNPFRPSAELDPGNPCI
                     AAITRATIRWNKLIPFWRYVPTSQKSLAGNGGLLFTKGIGEAPIVQMATFSIWKNLES
                     LNEFAYRSREHIGAIQRTRDIRWYREELFSRFQPYRTEGSWDGVELKL"
     gene            5295..>5975
                     /locus_tag="PK198_09415"
     CDS             5295..>5975
                     /locus_tag="PK198_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF38997.1"
                     /translation="MSMNSSQEKQQYDEAFGQVLAQLDAWQRRAVDQIEGPVVVIAGP
                     GAGKTHIMAARIGRILRDTDAKANNILCLTFTDAGVLALRSRLLQLIGPEAHRVHIFT
                     FHSFCNSIIQDNLDLFGRRDLEPVSELERIEITRRLLESLAPDHPLRQGHTDPHLYEQ
                     QLRELFRLMKSEDWKPEFVSMSIDAWLADLPQRSEYQYARSGKGLKKGDPKKALLQTA
                     SERMARLRA"
     assembly_gap    5976..6021
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6022..>7827
                     /locus_tag="PK198_09420"
     CDS             <6022..>7827
                     /locus_tag="PK198_09420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRF38998.1"
                     /translation="AARRYDYDDMILWVLRAFETNETLLRNYQEQYLYFMVDEYQDTN
                     GAQNEVLLQLARYWEEPNLFIVGDDDQSVYEFQGARLQNLTEFLESYRNSVELIVLPN
                     NYRSSQHILDAAAGVIQHNQLRVVHQFPELRIEKKLVAANPAVAALPQLPEWVVYPNR
                     LHEIIDIARQIETWRDEGVPLSEIAVIYARHRQAEALQALLERRGIPCQTKRKTDILK
                     LPMLRHWLEWLRYIETELRDPGSGEYYLFRLLHFHFTELPAAEINRLHLYLAAQSPAV
                     PRRTALSDAALLQAAGIRETEPFLRLSRSIDELMHSLANDTLPAFLERALHRSGLLAW
                     VLRQPDAVQLLEVMRTFFDFAELETLRRPQLSLMGLLSILDSFETNGVPIELQHNAVA
                     GKGVQLVTAHSAKGLEFERVFVLDAVKEEWEPRKRSGGGRFALPDTLTRSAEEDALEA
                     RRRLFYVAMTRAKTWLQVSYSMAGNAGKPLQAAVFVDELLEHSGREARQQTLSAEQIA
                     PGMAAMLSEAPPQIAEAPPLDIIAPLLEGFAMSITALNRYLRCPLEFYYQHILRAPAP
                     ATARAAYGTAMHFAVQRFFERMLADREKVFPDLAT"
     assembly_gap    7828..7837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7838..8463
                     /locus_tag="PK198_09425"
     CDS             <7838..8463
                     /locus_tag="PK198_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRF38999.1"
                     /translation="FFETEMRRLQGRFTPSQWEHYTNTGHSRLEALHRQEDWRRHRDV
                     KVEYTVRRTHLEGVPMTGTIDKIEFYDQLLVSVVDYKTGKPDAAKLAPPSSKNPYGTP
                     YWRQLAFYKLLYEHFDQSSRLVREGAITFLDPDAEGRFVTLRTSFDSHDMAMLKELIE
                     NTYARIMQQDFYTGCGKSDCLWCNFLRHNAPTETFTAPEIEDLDDAR"
     gene            complement(8534..10744)
                     /locus_tag="PK198_09430"
     CDS             complement(8534..10744)
                     /locus_tag="PK198_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39000.1"
                     /translation="MKQSYPSHFSVFRPCFWGLALLFSGHWATAQTTETVLADTLKTY
                     DMPEVRVLGEKPRLMSDIPGSASFIRPATLKALQPINGNEVLRTVPGLHVVEEEGIGL
                     RANIGIRGLDPDRSSRVLILEDGVPVALNPFGEPQMYYTPAIDRMEAVEVLKGSGQVL
                     FGPQTIGGGINYITAAPPQSEEARIRLQAGQGGYFSSLLSYGNTVGNAGFRVDYLRKQ
                     ADNIGSTGFKINDLNSKIKIRFSDRSTLGIKVGFYDEASNATYIGLTQTMYDRGGQDF
                     VRLAPDDLLTVRRISGSATHEYRFRPAVQLKTTAFAYSTTRDWRRQDFGRSPNVSNRT
                     GVIWGDESIPGGAIYMRNQNGHRDRQFEVVGVEPRLAASFQTGSVTHKLDAGVRFLFE
                     RAFEQRVNGTRPWARAGALVEDEIRTGRALSAFVQNRISLGQRWNVTAGLRSETYFYE
                     RDIARLAQKDTTILSNTTISQLIPGLGLNFQAADEWTIFAGLHRGFSPPRVKDAISNE
                     GVAYNLDPELSWNSELGLRGRIREWLTAELTAFYMDFSNQIIPISESSGGSGTGLANG
                     GRSLHRGLEWAVNWDAGSLFLPKGHSIEAYFSGTYSDAFYNADRFIGAGDAVNIRGNR
                     LPYAPRWMYSAALQYAAPFGLQGRVTVNGQSAQFTDELNTAISSADGETGLIEGRFTM
                     DGSLSYTLPNGKWSFIAGVKNLTNERFIATRRPQGIRVNVPRFFSLGLEWRLNK"
     gene            complement(10841..>12049)
                     /locus_tag="PK198_09435"
     CDS             complement(10841..>12049)
                     /locus_tag="PK198_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39001.1"
                     /translation="IELGKAYDYKASYSKFIALREERREKMMSAFENQQRVIAQKERT
                     INRFMAKASKTKMAQSMQKQLDKIDRIEIDPEDVAGMNLRFAPSPRSGQIVIEGHKLS
                     KSYGDKKVLDKVDIKLDRGDRVSFVGQNGQGKTTLAKIIVRELGLTDGDLQYGHNTQI
                     GYYAQNQSDTLQTNLTLLETMENASPPEMRPKLRAILGAFMFSGEDVDKKVSVLSGGE
                     RARLALACMLLKPFNLLVLDEPTNHLDIISKDILKQAVMDYDGTLIVVSHDREFLGGL
                     TTRTLEFRDRQLFEYLGDVNFFLQKRALRDMREVEMSKKAAAEAAATNESNLSQEERR
                     RLARNVQNAERAVEKIEADVAVFEAKMADPGFYESPDAQRVIDQYNLKKKELDEAMAK
                     WEEAQMAMDT"
BASE COUNT         2674 a         3154 c         3370 g         2782 t
ORIGIN      
        1 acggtgtttt ggccgagacg ctgctgtact tcggcgctgc ggtgctgctg atggccctgt
       61 tgatgggcgt gttcatgtac ttcatgcggc agaccatcat tgtgatgtcg agattggtgg
      121 aatatgacct gcgcaagtcc ttgtttgagc attacgagcg gctgcacacg ggctttttta
      181 agaaaaacaa cacgggggat ctgatggcgc gggtaacgga ggatgtgtcg aaagtgagga
      241 tgtacatggg gccggcggtg atgtacggcg tgaatcttat ctccttgttt atcatggtga
      301 tatatagcat ggtggcggtg agttnnnnnn nnnnnnntga cgctgtactg cctggcgccg
      361 ctgcccatcc tgtcggtttc gatttattac gtcagtcaga tcatcaataa aaagagcgaa
      421 cggatacagc agcaattggc agtgttgaac agtacggcac aggagagttt ttcgggtatc
      481 cgggtggtga aggcgtatgt gcaggagcgg gcgatgggtc gttttttttc ggaacagagc
      541 gaggattttc ggcagaagtc gttgtcgctg ggcaaggtga atgcgctgtt ttacccgctc
      601 atgttgctgc tcatcggggc cagcaccatt attacggtgt atgtgggcgg cttacaggct
      661 gtgcggggcg ccattacgac gggcaacatc gcctaatttg tcatttatgt aaacatgctg
      721 acctggccgg tgactgccat cggctggatt gcctcgatca tgcagcaggc ggctgcctcg
      781 caaaagcgca ttgacgagtt tttgaaaaca gagccggcga ttgccaatcc tgctcatgcg
      841 cccggtccgc tgaaagggca tatccgtttc gatcaggtca attttgtcta tcctgatacg
      901 ggcgtgcagg cgcttaaaaa tgtgagtttt gaattgaagc ccggccaaaa aatggctatt
      961 gtgggccgaa ccggatcggg gaaaacctcg gtggccgatc tgctggtgcg catgtacgat
     1021 gtttcgggcg gtcaggtgtt gatagatggc caggatgtgc gggcgcatga tttggacaac
     1081 ctgcgcagac gcaccggcta tgtgccgcag gatggattcc tgttttccga ttcagtgtcg
     1141 gggaacattg cttttggaaa acccgaagcc gagcagtctg aaattgagcg atttacaaaa
     1201 tatgctgcta tccacgacga aatcatgggg ctgccggagg gctatggtac agtggtggga
     1261 gagcggggtg tgacactttc cggcgggcaa aagcagcgca tttcaatagc gcgggcgctc
     1321 atcaaacagc ccgatatttt gctgctcgat gattgtcttt cggcggtaga tacgaatact
     1381 gaaaagcaga tactggcttg tttcaacgag atcatggcag ataaaacggc cattgtgatt
     1441 acgcaccgga tttactcata tcttcagttt gataagatca ttgtgttgga tggaggacgc
     1501 attgtggagg agggcacgca cgatgaattg ctggcagcga aggggtatta ttatgagatg
     1561 tttgagcggc aggcggtaga gccggaagct actttgtagt ttatttcctt cggagtaatc
     1621 tgctgcgcag ttcatctcct tcggagtaat ctgctgcgca gtttattgct tttcaaaaaa
     1681 atcatctgct cagctattga cattttccct gaaacttttt tatctttgcg ccgctttaat
     1741 agaaagcatt acacggtggt tgtagctcag ccggttagag cgtcggattg tggttccgaa
     1801 ggtcgtgggt tcgagcccca tcatccaccc cgccaaagcc gcttcgatct gtttcgaggc
     1861 ggctttttca ttttcctcca ctgtttctcg tgttgtcgta atttttttat taacaatttt
     1921 cacttgtatg aagcgttgat tagctttgcg cttcaaaacg aaaacaacaa aatcatcacc
     1981 catgctggaa aacatcattc tgctgttcca atccaccgac atcgtacgcg agccggaagt
     2041 agtacaacaa ggctcacaaa gccttttcga catcatccgt tccggcggcc ctatcggcat
     2101 gaccatcgta gcgatgcaat taggtatgtc gtttttggct ttctacatct tcatcgagcg
     2161 ttttctcacc atcaaaaaag cctcgcagat tgatgagagt tttatgaaca acatccgcat
     2221 caacgttcag tcaggcaata ttcagggcgc caaagccctc tgccagagca ccgattcgcc
     2281 ggtggcccgc atggtggaaa aaggtctgat gcgcatcggc aaacccctgc gcgacataga
     2341 cgccgccatc gagaacgtag gcaacctcga aatttttaag ctcgaaaaaa atctctccag
     2401 cctcgccacc atcgccggcg catcgcctat gatcggcttc cttggcaccg taacgggtat
     2461 gatcatcgcc ttctaccaaa tggcatcgca gcaaaacgtg acgccaggtg ttctggccgg
     2521 cggtatttat caggctctga ttactacggc tttcggtctg gtggtgggta tttttgcatt
     2581 ggtagcgtac aacatactgg tagccaatgt agaaaaagtg gtgttcaaaa tggaacgcag
     2641 taccaccgag ttcatggatc tcttgcagga gcctacgctt tgatctcgct ttcggggctg
     2701 agaagcaccc gtatttatcc acattccaat cgttaaaaca actcgatcat gggattaaaa
     2761 aagcgaagcg gcgtaacggc ggaattcagt ctggcatcgg ctaccgacgt catctttctg
     2821 ttgttgctgt tttttatgct cacctctact ttggtaaaaa tacagccgtt tgatctgcct
     2881 caatccgacg ctaaaacagt ggcgccggtg acggtggttg tcaccattcg gaaggacggc
     2941 tcgcatacgc tcaacaacaa cgacattgac cctaaggcca tcgagcgcgc tttgggcact
     3001 gctttggcag atgcaggcag cgaagcagaa gccaccgtca ccatcgtggc cgaaaccggt
     3061 acgcccttca gccacgtcgt caatgtaatg gaggctgcct ccaaaaacaa ggcccgcgcc
     3121 atcatcgcca cccaacccaa aagttgaagc ctaaaactgt tttgccatgt caatgaaaaa
     3181 acgcgccaga gtcaccgccg acttcaacat gtcgtccatg accgatatca tattcctgtt
     3241 gctcatcttc ttcatgctca cttcctctgt ggtagcgccc aacgctctca acctcaagct
     3301 gccgggcact tcgcgtgcca aagtggtgaa gcaaaaacaa gtggacgatg tgcgcatccg
     3361 caccagcggg gctttcgcac tcaacggcag gagcctggac ctcggaagtc tggagcagga
     3421 attggcccgc aaagcttcgg gcagcagcga gtttaccatc actatttcgc ccgatgacga
     3481 tacgcccaca gaacatgtgg tggccgtgat ggacatagcc ctgcggtaca gcatcaacgc
     3541 cattttgact acggagaagc agcgataact tgttaaggtt ttgtcaagct gttctttaca
     3601 gaaaatgatt ttcaacttcg ggagcgttat aacgttacct gaacatattc ggacccaaat
     3661 ccggtaaaaa gcaatcatca tggcattcga cactttagtc agcagagaag aacctaaaaa
     3721 caggcaaagg agctttgtca tcagcgtttc cattcatgtg gtgctgttgt ttttgctgtt
     3781 tgcatctttt ctcacattcc ccgacccgcc tccgggtcag gaaggtattt tggtcaatct
     3841 cggcctgccc gacgtaggcc agggcgatga aaacgccgct gccggcacgc ccgatgtggc
     3901 cgagccgact ccgcagccta aagaagaggc cgcgccgcct aaaaccgaac ctgccgccaa
     3961 gccggaacct gtgaaagaaa aggaggtgcg cgtcacggaa gacccggaag ccatagcgct
     4021 gaagcgcaaa caggagcagg agaaaaaagc acaggcagaa gccgacgcca agcgcaaagc
     4081 tgaggccgac gccaaattaa aggcagaagc cgaagcaaaa cgcaaagctg atgaaaaagc
     4141 tgccgccgat aaagctgctg cggataagct caaaggcgaa ttaggcggct tgctgggcag
     4201 tggcaaaggc aagggcaaca ccggcactcc cggcaatcag ggcgatccca acggcgaccc
     4261 caatgccgat aaactcaccg gcgtgagtac gggcataggc aaagtcggcg gcggattggg
     4321 cggacggaaa gtagcctcgg cgccaaaaat caccgacgac tctcaagacc ggggcaccgt
     4381 agtgttgcgc gtttgtgtgg atgcagatgg caacgtgacc agcgcaacgt ttacgcaggc
     4441 gggttcaaca gcgacgtcca ccaagctcaa aaacctcgct attgataatg caaagcgctg
     4501 gaaattcggc ccgggtgaag atggccagtg cggcaccatc acttacaact ttacagtaca
     4561 ataagcgtgg gcagtattgc cactattact tttttccgct ttcgcggaat ccgacatcaa
     4621 ttctggggtt tcacccaaat gcagtttgcc cacaaagccc tcggcgcgct tcgtgggcaa
     4681 actttttatc gcctgatggg cagtgggaga ggggaggggt tcaatccctg gcccgactgg
     4741 tcggtgtatg cgctgctgca agtgtgggac aacgaagctg aggccgccgc tttttttgcc
     4801 ggttccgacc tcatggggca gtaccgtcgc cacactactc aaatatggac aatttacatg
     4861 cggcccatca tggcgcgggg cgagtggtcg ggcagcaatc ctttccgacc ttctgcggag
     4921 cttgatcccg gcaatccctg tatagcggcc atcacgcggg ccaccattcg ctggaacaag
     4981 ctgattcctt tttggagata tgtgcccacc tcgcaaaaat cgctggcagg caacggcggt
     5041 ttattgttca ccaaaggcat cggcgaggcg cccattgtgc aaatggccac tttcagcatc
     5101 tggaaaaatc tggaatccct caacgagttt gcctatcgca gcagagagca catcggcgcc
     5161 atacagcgca cccgcgacat acgctggtat cgggaggagt tgttttcaag atttcagcct
     5221 tatagaacgg agggtagctg ggatggtgtt gaattgaaat tgtaacgtta tcttgtcggc
     5281 tcaaaccggc aggcatgagc atgaattcgt cgcaggagaa gcagcaatac gatgaagcct
     5341 ttgggcaggt gctggcgcag ttggatgcct ggcaacggcg ggctgtggat cagatagaag
     5401 gtccggtggt ggtcattgcc gggccgggcg cgggcaaaac gcatatcatg gcggcccgca
     5461 tcgggcgcat cctgcgagat accgacgcca aggccaacaa catactttgc ctcacgttta
     5521 ccgatgccgg ggtgctggcg ctgcgcagcc gcttgttgca actcatcggc ccggaggcgc
     5581 atcgggtgca tatttttact ttccactctt tttgcaacag catcatacaa gacaatctcg
     5641 acctcttcgg caggcgcgat ctggagccgg tgtccgaatt ggaacgcatc gaaatcaccc
     5701 gcaggctgtt ggaatcgctg gcgcccgatc atccgctgcg gcaggggcat accgatccac
     5761 atttgtacga gcagcagttg cgggagttgt ttcggttgat gaaatcggaa gactggaaac
     5821 cggagtttgt ctcgatgtcc attgatgcct ggctggccga tttaccacaa cgatctgaat
     5881 atcaatacgc tcgttcgggc aagggtctca aaaaaggcga tcccaaaaaa gcgctgctgc
     5941 aaaccgcctc cgaacgcatg gcccgcctcc gcgccnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn ngggcagccc gccgatacga ctacgacgac atgattttgt
     6061 gggtgttgcg cgcttttgaa accaacgaaa cgctgctcag aaactatcag gaacagtacc
     6121 tctacttcat ggtggatgag taccaggaca ccaacggcgc gcaaaacgag gtactcctgc
     6181 aattggcccg gtattgggaa gaacccaacc tgttcatcgt aggcgacgac gaccaatctg
     6241 tgtatgagtt tcagggcgcc cggctgcaaa atctgaccga gtttctggag agctaccgca
     6301 acagcgtaga attgatcgtg ctgcccaaca actaccgctc ctcgcagcac atcctcgatg
     6361 cggcggcagg cgtgatacaa cacaaccaat tgcgggtggt gcatcagttt cccgaactgc
     6421 ggattgaaaa aaaactcgtg gctgccaatc cggctgtagc cgcgctgccg caattgcccg
     6481 aatgggtggt ttatcccaac cgtttgcacg aaatcataga cattgcccgg cagatagaga
     6541 cctggcgaga cgagggcgtg ccactctcgg aaatagccgt gatctatgcc cggcaccggc
     6601 aggcagaggc attgcaggcg ctgctggaac ggcgcggaat cccctgccag acgaagcgca
     6661 aaaccgacat tttgaaactg cccatgctgc gtcattggtt ggaatggctg cgttatatag
     6721 aaaccgagct gcgcgacccc ggcagcggcg agtactacct gttccgattg ctgcatttcc
     6781 attttacgga acttcccgcc gccgagatca accggctgca cttgtacctg gctgcccaat
     6841 caccagccgt gccgcgcaga accgccctga gcgatgcggc gctgttgcaa gccgccggta
     6901 ttcgggaaac cgagcctttt ttgcgcctgt cgcggagcat agatgagttg atgcacagtt
     6961 tggccaacga cacgctgcct gcatttttgg aacgcgccct ccatcgcagc ggcctcttgg
     7021 cttgggttct ccggcaaccc gatgcggtgc aactcctcga agtgatgcgc acttttttcg
     7081 attttgcgga actggaaacg ctgcgccgcc cgcaactcag tctgatgggt ttgttgtcta
     7141 tcttagacag ctttgaaacc aacggtgtac ccatagaact acaacacaac gccgtggccg
     7201 gcaagggcgt gcaactcgtg acggcccaca gcgccaaggg gctggagttt gagcgcgtat
     7261 ttgttttgga tgcggtgaaa gaagagtggg agcctcgcaa acgcagcggc ggcgggcgtt
     7321 ttgcattgcc cgacaccctc acccgctcag cggaagagga tgcccttgaa gcgcggcgac
     7381 gactgtttta tgtggccatg acccgtgcca aaacctggct tcaggtgtcg tacagcatgg
     7441 ccggcaatgc cggaaagccc ttgcaagctg ccgtttttgt ggacgaattg ttggagcaca
     7501 gcggtcggga agcccgccaa cagaccttga gcgccgaaca aatagcgccg ggcatggccg
     7561 ccatgttgtc ggaagcgccg ccgcaaatcg ccgaagcgcc gccattggac atcatcgcgc
     7621 ccttgctgga gggatttgcc atgagcatta cggcgctgaa caggtatttg cgctgcccgc
     7681 tggaatttta ttaccaacac atcctccgcg cacccgcgcc tgctactgcc cgcgctgctt
     7741 acggaacggc catgcacttt gcggtgcagc gtttttttga aaggatgctg gccgaccggg
     7801 agaaggtgtt tcccgatttg gccacgcnnn nnnnnnnatt tttttgaaac ggaaatgcgc
     7861 cgtttgcagg gccggtttac gccctcgcag tgggagcatt acacaaacac agggcacagt
     7921 cggctcgaag cgctgcaccg gcaggaggac tggcgccgtc accgggatgt aaaagtggaa
     7981 tataccgtgc gcagaaccca cttggagggc gtgccgatga cgggaacgat agataaaatt
     8041 gaattttacg atcaactctt agtctcggtg gtggattaca aaacgggcaa gccggatgct
     8101 gccaaattgg ctccgccgag ttccaaaaat ccttacggaa cgccgtattg gaggcagttg
     8161 gccttttaca agttgttgta cgagcatttt gaccaaagct cccgccttgt gcgcgaaggg
     8221 gccatcactt ttttagaccc cgatgcggag ggccggtttg tgaccctgcg cacgagcttc
     8281 gacagccacg acatggccat gctgaaggaa ttgatagaaa atacttatgc ccgcatcatg
     8341 cagcaggatt tttacaccgg ctgcggaaaa agcgattgtc tgtggtgcaa tttcctgaga
     8401 cacaacgcgc cgacagagac ttttactgcg ccggaaatag aggatttgga tgatgcgcgg
     8461 tgagtggcaa agagtgaagg agtaactaca ggcctatgct tggctgagtt gccccttcac
     8521 catcctccca attttattta ttcaacctcc actccagacc aaggctgaaa aatcgcggca
     8581 cattcacccg tatgccctgc gggcggcgcg tggcaatgaa acgctcgttc gtcaggtttt
     8641 ttacgccggc tatgaagctc cattttccgt tgggcaaagt ataactcagg ctgccgtcca
     8701 tcgtgaatcg cccttcgatc agaccggttt cgccgtcggc ggaggaaatg gcggtgttca
     8761 gttcgtctgt aaactgcgcg gattgcccgt ttacggtcac acgaccttgc aaaccaaaag
     8821 gagcagcata ttgcagcgcc gccgagtaca tccatcgcgg ggcgtaaggc agtcgattgc
     8881 cgcgaatatt gacggcatcc cctgcgccga taaagcggtc ggcattgtaa aatgcatccg
     8941 aataagtgcc tgaaaaataa gcctctatgc tgtgcccttt cggcaaaaac aggctgccgg
     9001 catcccaatt gacggcccac tcaagacccc ggtgcaagga gcgtcctcca ttcgccaatc
     9061 ccgtgccgct gccgcccgat gattcggaaa tggggatgat ctggttggaa aaatccatgt
     9121 aaaatgccgt cagttccgcc gtgagccact cccgtattct gccgcgcagc cccagttcgc
     9181 tgttccaact caattcgggg tctaagttgt atgccacgcc ttcgttcgag atcgcgtctt
     9241 tcactcttgg cggcgagaag cctctgtgca ggcccgcaaa aatcgtccac tcgtcggcgg
     9301 cttggaagtt cagccccagt ccgggaatga gctgagaaat ggtggtgttg gaaaggatgg
     9361 tcgtgtcttt ttgagctaag cgggcgatgt ctcgttcgta aaaataagtt tccgaacgca
     9421 atcccgccgt gacattccag cgctgcccca gcgaaatgcg gttctgaaca aaagcgctca
     9481 acgcccgccc cgtgcgaatc tcgtcttcta ccaaggcgcc ggcccgcgcc cagggtcttg
     9541 ttccgtttac ccgctgctcg aaagcccgct cgaaaagaaa gcgcactccg gcatcgagtt
     9601 tgtgtgttac cgaacccgtt tgaaacgaag ccgccaatcg gggttctacg cctacaactt
     9661 caaactgcct gtcgcggtgg ccgttttggt tgcgcatgta aatagcgccg ccgggaatgc
     9721 tttcatctcc ccagatcact ccggtgcggt tgctcacatt ggggctgcgg ccaaaatcct
     9781 gccgccgcca gtcgcgggtg gtgctgtagg caaaagcggt ggttttgagc tgcaccgcgg
     9841 ggcgaaaacg gtactcgtgg gtagcgctgc cggagatgcg gcgcacggtc agcaggtcgt
     9901 cgggggcgag gcgcacaaag tcctgtccgc cccggtcgta catggtttgc gttagcccga
     9961 tgtaggtggc attggaggct tcgtcgtaaa atccgacttt gatgccgagg gtagaacggt
    10021 cgctgaaacg aatttttatc ttgctattca agtcattgat tttgaatcca gtagaaccga
    10081 tgttgtcggc ctgcttgcgg aggtagtcca cccgaaaacc ggcgttgccc accgtattgc
    10141 cgtagctgag caggctgctg aaataaccgc cctgaccggc ctgcaggcga atgcgcgcct
    10201 cttccgattg gggcggcgct gccgtgatgt agttgatgcc cccgccgatg gtttgcggcc
    10261 cgaacagtac ctgcccgctg cccttgagta cctccacggc ttccatgcgg tctatggccg
    10321 gggtgtaata catttgcggt tcgccgaaag gattgagcgc tacgggtacc ccgtcttcca
    10381 gtatgagcac gcggctgctg cggtcagggt cgaggccgcg aatgccgatg ttggcgcgca
    10441 agccgatgcc ctcttcctcc actacatgca ggccgggtac cgtgcgcagc acttcgttgc
    10501 cgttgatggg ctgtagtgct ttcagcgttg ccgggcgtat gaaacttgcc gagccgggga
    10561 tgtcgctcat caggcggggt ttttcgccca agacccgcac ttcgggcatg tcgtaggttt
    10621 taagggtgtc ggcgagcact gtttcggtgg tttgcgcagt ggcccaatgg cccgaaaaca
    10681 ggagcgcgag tccccaaaaa caaggtcgaa acacagaaaa gtgagatgga taagactgct
    10741 tcatttttat ttagattaaa tttaaataat gaagcaaagg tacattggca gtactcacac
    10801 cggcaatacc tacgaatgag gaaattttgg gtagctaatg ttaggtatcc atcgccattt
    10861 gcgcttcctc ccatttggcc attgcctcat ccaactcctt ctttttcagg ttgtactggt
    10921 caatgacgcg ctgggcatcg gggctttcgt agaagccggg gtcggccatt ttagcttcaa
    10981 aaactgctac atcggcttcg attttttcta cggcacgctc ggcgttttgc acattgcggg
    11041 ccaggcgtct gcgctcttcc tgagataggt tggattcatt tgtggcagca gcttcggcag
    11101 ccgctttttt cgacatttcc acttcgcgca tatcgcggag ggcgcgtttt tggaggaaga
    11161 aattgacatc gccgaggtac tcaaaaagct ggcggtcgcg gaattcgagg gtgcgggtgg
    11221 taagacctcc gagaaattcc cggtcgtgag aaaccacgat gagggtgccg tcgtagtcca
    11281 tcacagcctg tttgaggatg tctttggaga tgatgtccaa gtggttggtg ggttcgtcaa
    11341 gtaccaacag attgaagggt ttgagcaaca tacaggctaa ggccaatctg gcgcgctcgc
    11401 caccggaaag cactgatact tttttatcca catcttcgcc gctgaacata aaagcgccga
    11461 ggatggcccg cagtttgggg cgcatttcgg gtggagaggc gttctccatg gtttccagca
    11521 gggtgaggtt ggtctggagt gtatccgatt gattttgagc gtagtatcct atttgtgtat
    11581 tgtgtccgta ttggaggtct ccgtcggtga ggcccagttc gcgcacgatg attttggcta
    11641 aggtggtttt gccctgcccg ttttggccca caaacgacac ccggtcgccg cggtcgagct
    11701 tgatgtccac cttgtcgagc acttttttgt cgccgtagct tttgctcaac ttatgccctt
    11761 cgatgacgat ctggccggag cggggcgagg gtgcaaatcg gaggttcatg cccgctacgt
    11821 cttcggggtc tatttcgatg cgatctattt tgtccaactg cttttgcatg gattgcgcca
    11881 tcttggtctt ggaggctttg gccataaagc ggttgatggt gcgctctttt tgtgcaatga
    11941 cgcgctgttg gttttcaaaa gccgacatca ttttttcgcg gcgttcttcg cgcagggcta
    12001 tgaatttgga gtaagaagcc ttgtagtcat aggctttgcc gagttctat
//
LOCUS       DAORLF010000092        11989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2845_length_11989_cov_2.319172, whole genome shotgun sequence.
ACCESSION   DAORLF010000092 DAORLF010000000
VERSION     DAORLF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_11989_cov_2.319172"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..>863
                     /locus_tag="PK198_09440"
     CDS             45..>863
                     /locus_tag="PK198_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39002.1"
                     /translation="MTIFQKYVSLPGFLLLLASVPLSAAAPFRYEITPKAQNAYELTT
                     SLRFTEAGVVVAQIRAKDPGNLIPYHLENYADCLAVYISEDENAYNRLKRKKSERLAA
                     VQQCDPHSPYFRFVQADMRLQWALAHLKFGDYFSAFSETGKAYKLLKKNQELFPSFLP
                     NQKDLALLHAIAGVVPDEFKWGMKFLTGLEGSIAQGRREMEAVLHKSRSTHFLFETET
                     RIMYAFLLLHLANDGQAAWQAVSKDQLQPAQNPVHCFIVANIAMRTGRNDKAIEL"
     assembly_gap    864..873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <874..1556
                     /locus_tag="PK198_09445"
     CDS             <874..1556
                     /locus_tag="PK198_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39003.1"
                     /translation="RCPRDNRHIPLPFIDQMLGVAKLRRLDPDADKYMIRFLQQHKGR
                     NFIKDAYQKLAWHSLVHGDKKGYRQNMEYCLQKGHATIGNDRSALREARSEHLPHIIL
                     LRARLLFDGGYFQKALEVMKNESATAFEQERHRIEYAYRMGRIHHSLGQMDTAMKFYQ
                     SALQQGRAQGSFFACNACLQIGLIYEQLRQPVQAKEYFQRCLSLESDEYETELHQKAK
                     AGLNRLKG"
     gene            1732..2961
                     /locus_tag="PK198_09450"
     CDS             1732..2961
                     /locus_tag="PK198_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39004.1"
                     /translation="MLFQRSLYPHVENHLPDKRHTIITGARQVGKTSLLRLIHQKLKA
                     EGHLVFQLSLEDGKVLSDINTHPDNVFLHIPALPTPAQNGQAEKRLYLLIDEVQYAAD
                     PTRFLKFLYDKYEGNLKVVATGSSAFYIDRKFTDSLAGRKRVFQLYTLSYEEYLVFIG
                     RNDLAEEVNMMRERPEYISSHSTALKAVFYQYLIFGGYPAVATEPDLLEKKLLLDELK
                     NAYVKRDIAESGVSMEAKFFLLFQLLADQTGNLVNRHELSKTVQVDFKTIENYLYVLE
                     KCCHIHLMKPFFRNIQKELTKMPKVYFNDLGLRNALLNRLDPIENRSDKGALLENYFY
                     CRLRQQYETDQLKFWRTADQHEIDFVAETSFGQGRAYEVKWNKRNFSAEKLKKFTAAY
                     PAFALECLDETDFVSLL"
     gene            3067..3534
                     /locus_tag="PK198_09455"
     CDS             3067..3534
                     /locus_tag="PK198_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39005.1"
                     /translation="MRIIATLLLMATLAAPIFAQETALPKVFILGEEEQVYEALTRDY
                     QETLLSACDGNMETAVDKWLGMMQEMENYAKKIRFELKGVKLWMHVFWDADGSVKHIG
                     FRLRPDSRNIRTEDLAAFLVSFMNRYKMPLSFDKKYAHYVGATFPTFVERLEK"
     gene            3651..4612
                     /locus_tag="PK198_09460"
                     /pseudo
     CDS             3651..4612
                     /locus_tag="PK198_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            4674..5255
                     /locus_tag="PK198_09465"
     CDS             4674..5255
                     /locus_tag="PK198_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF39006.1"
                     /translation="MKNNIIIAAIAALLVATAFLTSNCNQNQGGTQSAAQAKETGPKI
                     VFVNADTLLANYEYMNQKQEEFGQREAKADADLKARGRALEKEFMAAQEKIQKGLLAP
                     NQIAVEEQRLGQKQQALAAEQERLTRELMAEGQKLQEELNKTIKSLLADIQKEKGYDY
                     ILSYGPGTGVLMVNDSLDITQMVLERLNKKEEK"
     gene            5343..5675
                     /locus_tag="PK198_09470"
     CDS             5343..5675
                     /locus_tag="PK198_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF39007.1"
                     /translation="MMKNILLFTVVALVVIACGNANSGSAAMAAAEEQPSEGAKIYKQ
                     YCLTCHGLYGDMGASGAANLQESKLTLEQRVEVITNGRNVMTGFKALMSEDKIKLVAQ
                     YTFELKNN"
     gene            5694..6053
                     /locus_tag="PK198_09475"
     CDS             5694..6053
                     /locus_tag="PK198_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39008.1"
                     /translation="MKNTCVECGEEIKGRADKKFCSDQCRSAFNNRLNSDATNFIRNI
                     NNILRKNRRILAELNPDGKTKIHRDRLMERGFKFSYFTNEFVTRTGNVYRFCYDQGYL
                     ALDEGWFALVVRQEYVE"
     gene            complement(6063..>6837)
                     /locus_tag="PK198_09480"
     CDS             complement(6063..>6837)
                     /locus_tag="PK198_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39009.1"
                     /translation="ISEPYRMPVLLEELSDKRPFLLAVSASKLREDQNVSRLYGHLLY
                     GAKLMYEKDILQIYEVPLELFETRIRQRREALTAAAQSDSLYEIAPFKVDNPNFTYVY
                     EPFDDRPSKRSYLGGGAWSGEAGKENVLYDGTVPKQWPGGMYHFSAWAYVGDDLAART
                     SMQFEEYNPTTGEVYQKHTTWLFFIFGLVDPGGWTLIEYPFTPVAADSKLRFTFQNKM
                     MGRRPIYIDEVLVRATAAHTYRLNDKGLWYNNRWFPKGE"
     assembly_gap    6838..6929
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6930..>7279)
                     /locus_tag="PK198_09485"
     CDS             complement(<6930..>7279)
                     /locus_tag="PK198_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39010.1"
                     /translation="KQFRSIGRFAWVFFYVINIVAFLWLYSATKSKPWQPVVMGAAIA
                     LLSWEAWNFQKMFDLRLDEVEEFKTGQQFTDLKEIDFSRYQAILTVPYFNIGSDNLGA
                     FGNDGFILQKSLTL"
     assembly_gap    7280..7419
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7420..8388)
                     /locus_tag="PK198_09490"
     CDS             complement(<7420..8388)
                     /locus_tag="PK198_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39011.1"
                     /translation="MRNSAFFYLGLLLTTALMALLLYLRLPDFPQHAGTRVIEPYGDG
                     YKAYATYLYHIRHDSTNTWFQGMNYPYGEHVIPSDTQPFLSNGIRFVNRYITDISGAA
                     LGIFNWSMPIGVLLCGIFLYLIFARLGLPVVYSVPIAVALSFLSPQLWRMISHFGLAH
                     PEVLPILLYLLLRFDEKPSWKISALIAFSIAVFSQLHFYYFAIMGMTIGLYFFFRVLQ
                     RREWKQIPLWAAHVGLQVVVPGIVFFAWMADGITDRTSQPAGFLNYRSIWESVFTSFS
                     QPYWKWIDEHVIRIEQGDYEGLAYAGLAAGLAVLLMLYRMFVGKFRK"
     gene            complement(8398..10422)
                     /locus_tag="PK198_09495"
     CDS             complement(8398..10422)
                     /locus_tag="PK198_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRF39012.1"
                     /translation="MQFLFPTFLFALAALAIPIIIHLFYFRKFKKVYFTNVRFLREVK
                     EETSARSKLRNLLVLLSRILALTGLIFAFAQPFIPQDAEVKAGNKAVSVFIDNSFSMS
                     ALSQDVPLVEKAKQRAREIVEAYAATDRFQILSNDFEGRHQRLVSKEDALALIEEIKI
                     SPSVRNLTEVVTRQKQALASGSEEVETAYLISDFQKNVTDLKNWPDTLPEINLVPLQS
                     VQERNIAIDTAWFEAPVQMIGQTNTLIVRVRNYSNQAAENIRLAIKYDGQTKPAGTMS
                     IPAGAVATDTVSLTVLRTGWHEAELSVTDYPVQFDDKYFFSFHVAEQVNVLVINEESA
                     NRYLDAAFAGIPYFKAVNMSVRNLEFSQFPNYQLIVLNETREISSGLAAELNRYARAG
                     GNVLVFPSRNAGIAGYNSFLQGFGAASLQAYEDGVKNVGNINTSEFVFRDVFDNKSAN
                     LKLPSVKGSFRSERSGTGETLLAFRDGSAFLSKYQADQGHLYFCVAPLDETQSDLVRN
                     GEIFIPLLYKTAISSARNLRIAYTISRAETLESDHKVEGNQIVYKLRGPSGEFIPEQR
                     IVGSRVFITTANQIQQAGFYDLFLQSEAVINKFAFNFDRKESDLACYVPDELQTFVGD
                     NIKVLEINEEAILTAKIEERSQGISLWRWFLVMALVFLGVEVLLLKFWKV"
     gene            10557..11528
                     /locus_tag="PK198_09500"
     CDS             10557..11528
                     /locus_tag="PK198_09500"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRF39013.1"
                     /translation="MISADAIAAVNRLAEEGRPFCFAFNFEADEALIGADEDWARQGI
                     LFQMGDFSNFTPRETEAAPADWLPQPVSYETYRQAFEVVHGHLLAGNSYLVNLTFPTP
                     VQTGMSLETIFHRSRAPYKMLWPGRFAVFSPESFVRIRNGLISTFPMKGTIDADLPDA
                     ASKILADRKELAEHVTIVDLLRNDLNRVATDVRVQRFRYLDRIETNRKNLLQVSSEIS
                     GRLPGDYRPRLGNILAELLPAGSVSGAPKAKTLEIIQEAEGAPRGFYTGVMGRFDGQN
                     VESAVMIRFVEQTATGLVFRSGGGITVFSKPQDEHNELIDKVYVPIA"
     gene            11512..>11989
                     /locus_tag="PK198_09505"
     CDS             11512..>11989
                     /locus_tag="PK198_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF39014.1"
                     /translation="MCRLLETICFIDGAPPLLPYHQARFDAARRHFWGALPPLLLKEV
                     LVVPPDFRQSKVKCRLTYSAADFVVEWEHYTPRRIGSLQIVSGDHLDYAFKYADRSAL
                     HELWLQRGAADDILMVKNGLITDTSYSNVALFDGTRYFTPAQPLLAGVRRAALLDAG"
BASE COUNT         2949 a         3133 c         3089 g         2576 t
ORIGIN      
        1 aggattgacg ccagagatat attccaatat caaagaccgg gaagatgacc atattccaaa
       61 aatatgtttc gcttcccggt tttttgttgc tgctcgcgtc ggttcctttg tcggcggcag
      121 ccccttttcg ctacgaaatc acgcccaaag cccaaaacgc ctacgaactc actacgagcc
      181 tgcgcttcac ggaggctggt gtagtggtag cacagatcag ggccaaagac cccggtaatc
      241 tcattcctta ccatttggaa aattatgccg actgtctcgc cgtgtatatc agcgaagatg
      301 agaatgctta caacaggctc aaacgaaaga aaagcgaacg tttggccgcc gtgcaacaat
      361 gcgacccaca ctcgccatac ttccgttttg tgcaggccga catgcgtttg cagtgggctt
      421 tggcgcatct caaattcggc gattatttct ccgctttttc cgaaacgggc aaagcataca
      481 agctgctgaa aaaaaatcag gagttgtttc cctctttttt gcccaatcaa aaagacctgg
      541 cgctgctgca tgccatagca ggcgtcgttc ccgacgagtt caaatgggga atgaaatttc
      601 tcaccgggct ggaaggcagc attgcgcagg gccgccgcga aatggaggct gttttgcaca
      661 aatcccgctc cacgcacttt ctgtttgaaa ccgaaacccg catcatgtac gcctttttgc
      721 tgctgcactt ggccaacgac gggcaagcgg cctggcaggc agtgtctaaa gaccagttgc
      781 aacccgccca aaatccggtg cattgtttca ttgtagccaa cattgccatg cgtaccggca
      841 gaaacgacaa ggccatcgaa ctgnnnnnnn nnnaacgctg cccgcgcgac aaccggcaca
      901 ttccgctgcc gttcattgac caaatgctgg gagtggccaa actccgccgt ttagaccccg
      961 atgccgataa gtacatgatt cggtttttgc aacagcacaa aggccgcaat ttcatcaaag
     1021 atgcttacca gaaattggca tggcacagcc ttgttcatgg cgataaaaaa ggctaccggc
     1081 aaaacatgga atactgcctg caaaaaggtc acgccaccat cggcaacgac cgcagcgccc
     1141 tgcgggaagc gcgatcggag catttgccgc acatcatttt gctgcgcgca aggcttctgt
     1201 ttgacggcgg gtatttccaa aaagcgctgg aagtgatgaa aaacgaatcg gccacggcat
     1261 tcgagcagga acgccaccgc atagagtacg cctaccgcat ggggcgcatt caccacagtc
     1321 tgggccaaat ggatacggcc atgaaatttt atcaatcggc cctgcagcag gggcgggcgc
     1381 aaggttcgtt tttcgcctgc aatgcctgcc tgcaaatcgg attgatctac gaacaactcc
     1441 ggcagcctgt tcaggcgaaa gagtattttc agcgctgtct ttcattggaa tcggacgagt
     1501 acgagaccga actccaccag aaggccaaag ccggcttgaa caggctgaag gggtaggaga
     1561 gacggccata taaaacgtac cgcagcactg cgtcctctac gagatgagcg ctgcccttct
     1621 taggtaagac tgagcgcgat tcagcagaaa ttccaaaaat atctgataat tttggattct
     1681 gattccgtaa atatctgata attttgtatt tttaatccaa ttttatctgc catgcttttt
     1741 cagagaagcc tttatccgca tgtagaaaat catttgcccg acaaacgcca caccatcatt
     1801 accggggcaa ggcaagtagg taaaacctct ttgttgcgac tgattcacca aaaattgaaa
     1861 gcagaggggc acttggtttt tcaactatcc ttggaagacg gcaaagtgct ttccgacatc
     1921 aatacacacc ctgataacgt atttttgcac atcccggcac tgccgacacc cgcccaaaac
     1981 ggtcaggcag aaaaacgatt gtacctgctc atagacgagg tgcagtacgc ggccgaccct
     2041 actcgttttc taaagttttt gtacgacaaa tatgagggca atcttaaagt ggtagcaacg
     2101 gggagcagtg cattttatat agaccgaaag ttcaccgatt ctcttgcggg gcgcaaacgg
     2161 gtgtttcagc tttacacgct ctcttatgaa gagtacctcg tttttatcgg caggaatgat
     2221 ctggccgaag aagtaaacat gatgcgcgag cggcccgaat acatttcgtc gcacagcaca
     2281 gcactcaaag ctgtttttta ccaatacctc atttttggag gctaccctgc ggtggcgacg
     2341 gagccggatt tattggaaaa aaagctcctg cttgacgaac tcaaaaacgc gtatgtgaaa
     2401 agagacatcg ccgaatcggg agtgagcatg gaggccaaat ttttcttgtt gttccaactt
     2461 ttagccgacc aaaccggcaa cttagtcaat cggcacgagc tttccaaaac ggtgcaggtg
     2521 gacttcaaaa ccatagaaaa ctatctgtat gtactggaaa agtgctgcca cattcacctg
     2581 atgaagcctt tttttagaaa tatccaaaaa gaattgacca aaatgcccaa ggtctatttc
     2641 aatgatttgg ggcttcgaaa tgcactgctc aacaggcttg atcccataga aaaccggtcg
     2701 gacaaaggcg ccttgctcga aaattatttc tattgcaggc tgcgacaaca gtatgaaacc
     2761 gatcaactga aattctggcg aaccgccgac caacacgaaa tagacttcgt ggcagagacg
     2821 agcttcgggc agggccgggc gtatgaagtg aagtggaaca aacggaactt ctctgccgag
     2881 aaattgaaaa aatttacagc agcctaccct gcctttgcat tggagtgttt ggatgagaca
     2941 gatttcgtga gcctgctgtg aatcacttgc ttctgtaggc gggccgaccg gcaaccccaa
     3001 aatattgccc ccgctctgcc gtttttatac tatctttgaa attcaaaaat ccaatcgcac
     3061 caaattatga gaatcatcgc aacacttctt ttgatggcca cattggccgc cccgatcttc
     3121 gctcaggaaa ctgcgctgcc taaagttttt atcctcggcg aggaagagca ggtatatgaa
     3181 gccctgaccc gcgactatca ggagaccctg ctctcggcct gcgacggcaa catggaaact
     3241 gccgtggaca aatggctggg catgatgcag gaaatggaaa actacgccaa aaaaatacgc
     3301 tttgagctga aaggcgtcaa actgtggatg cacgtttttt gggatgccga cggctcggtg
     3361 aaacacatcg gttttcgcct gcgccccgac tcgcgcaaca tccgcaccga agacctggcc
     3421 gccttcttag tcagttttat gaaccgctac aaaatgcccc tcagcttcga caaaaagtac
     3481 gcgcattatg tgggcgctac tttccccact ttcgtggaaa ggttggagaa ataggtgacg
     3541 gctggtaatt attccgttac tttgcagcgc ttttcaaaac acggcatggc cctgcctgcc
     3601 gcaaacaacg aagagccgct gatgcttttt aaccaactta cacataagtc atgattaaga
     3661 ttcttatcaa cgacggcatc catcccgatg gacaaacgct gctcgaagaa gccggttatc
     3721 accttgatat agagcatgtg ccgcaagctg atctgcccaa agtgctgccc ggctacgacg
     3781 tcattttggt gcgcagtgcc accaaagttc gcaaagatct cattgacctc tgccccaatc
     3841 tgaaaatcat cggccgcgga ggcgtaggca tagacaacat agacgccgat tatgcccgca
     3901 gcaaaggcat cgaagtcatt accacgccgg cagcttcttc tcagtcggtg gccgaactgg
     3961 cattcgcgca catgttttcg ctggcccgct cgctgcaatt ggccaaccgt ttcatgccca
     4021 ccaaaggcga tcaggagttc aagcagatga aagaagccta ttccgacggc atacaactca
     4081 gaggtaaaac actgggtgtc attggttttg gccgcattgg tcaggaagcc gcccgcatcg
     4141 gtatcgctct gggaatgaaa gttatgccgg tggatttgtt tattgacgaa gccgacattg
     4201 acatctccat ccccgactcc gacaacgtga gcctttcggt caaaatgcgc acctacgaca
     4261 tggacgaagt actcaaacag gccgatttta tcacgctgca cgttcctttc ggcggcggta
     4321 cgtccatcat aggcgcgcct gaattggcca aaatgaaaac cggcgcctta tcaatacgtc
     4381 tcgcggcggt gtagtggacg aagaagccat gctggaagcg ctcaattcgg gcaaactcgg
     4441 cggcgcaggc ttagacgttt ttgacttcga accgaaaccg cgcaaagaaa tcctcgaaca
     4501 tccgcgcatc tccatgacgc cgcacatcgg cgcttccacg caggaggcgc agtcgctcat
     4561 cggcatggaa ctggcggata agattctggc atatttcgga gacgataagt aacattatcc
     4621 agagtgtccg gcaggctcaa tactgtcgga cgcttttcat aaccaacaga ttcatgaaaa
     4681 acaacatcat tattgccgcc atcgccgcat tgctcgtggc aactgccttt ctgaccagca
     4741 actgcaatca aaatcagggc ggaactcagt ctgctgccca agctaaagaa accgggccta
     4801 aaattgtatt cgtcaacgcc gacaccctgc tcgccaatta tgaatacatg aatcaaaagc
     4861 aggaggagtt tggccaacgc gaagccaagg ccgatgccga cctcaaagcg cggggccgtg
     4921 ctttggaaaa agagttcatg gccgctcagg aaaaaatcca gaaaggactg ctggcgccca
     4981 atcaaattgc cgtagaagag cagcgcttag gccaaaagca acaggcattg gccgctgaac
     5041 aggaacgcct gacccgcgag ttgatggccg agggccaaaa attgcaggag gaactgaaca
     5101 agaccatcaa aagtctgctt gctgatattc agaaagaaaa gggctacgat tacatcctca
     5161 gctatggccc gggcaccggc gtactgatgg tgaacgacag tctggatatt acccaaatgg
     5221 tactggaaag gctgaacaaa aaagaagaga aataatagag tttgagcgaa ggggtgaccg
     5281 aagttccccc ttcgttgagt accgataaat aaaaattacg ctgcactcat tttctcattg
     5341 ccatgatgaa gaacatctta ctttttacgg tggtagcttt ggtggtcatc gcctgtggaa
     5401 atgccaattc gggcagtgcc gctatggccg ctgccgagga acagccttcc gaaggtgcaa
     5461 agatttataa gcaatactgc ctgacctgtc acggattgta cggagatatg ggggccagcg
     5521 gcgccgccaa tttacaagaa tccaaactga cgctggagca acgcgtggag gtcatcacca
     5581 acgggcgcaa cgtcatgacg ggctttaagg cgctgatgag tgaagacaaa atcaaattag
     5641 tggctcaata cacattcgag ctgaaaaata actgattcac ccaccaaacc gccatgaaaa
     5701 acacttgcgt agaatgtggc gaagagatca agggccgggc agacaagaag ttctgttccg
     5761 atcagtgtcg cagcgccttc aacaaccggc tgaacagcga tgccaccaat ttcattcgca
     5821 acatcaataa catcttgcgc aaaaaccgcc gcatcttagc cgaactcaac cccgacggca
     5881 aaacgaaaat ccaccgcgac cgcctgatgg agcggggctt caagttcagc tatttcacca
     5941 atgaattcgt gacgcgcacg ggcaacgttt atcgcttttg ttacgatcaa ggctacttag
     6001 cgcttgatga gggctggttt gctttagtgg tgcggcagga gtatgtggag tgaggcgcgg
     6061 tactactctc ccttcggaaa ccaccggtta ttgtaccaca aacccttgtc attcaagcga
     6121 taagtatgcg cggcggtggc tcgcaccaat acttcgtcta tgtaaatcgg tcggcggccc
     6181 atcattttgt tttggaaagt gaagcgcagt ttgctgtcgg cagcaacggg cgtaaacgga
     6241 tattcaatca gcgtccagcc gccggggtcc accaatccaa agatgaaaaa cagccaggtc
     6301 gtgtgcttct gatacacctc gccggttgtg gggttgtact cctcaaattg catggaagta
     6361 cgggcagcca gatcgtcgcc cacatacgcc caggccgaga agtggtacat gccaccgggc
     6421 cattgcttgg gaactgtgcc gtcgtacaaa acattttctt tgccggcctc acccgaccat
     6481 gcgccgccgc cgaggtaaga gcgcttggag ggccggtcgt cgaagggttc atatacataa
     6541 gtgaagtttg gattgtccac cttaaacggt gcaatctcat acagcgaatc cgattgtgcg
     6601 gcagccgtga gcgcctctct gcgctgccgg atgcgggttt caaagagttc cagtggcact
     6661 tcgtaaatct ggaggatgtc tttttcgtac atcaatttgg cgccgtacag caggtggccg
     6721 tagaggcgtg atacattctg gtcttcgcgg agtttggagg cgctcactgc cagcaggaag
     6781 ggccgtttgt cggacagttc ctccaacaaa accggcattc gatacggctc gctgatgnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gagcgtcaat gatttttgaa ggataaaacc
     6961 atcgttgccg aacgcgccga ggttgtctga accgatgttg aaataaggca ccgtcagaat
     7021 ggcctggtag cgactgaagt caatttcttt caggtcggtg aattgctggc cggttttaaa
     7081 ctcttccacc tcatctaatc ggagatcgaa cattttttga aaattccagg cttcccaact
     7141 caaaagtgca atggccgcgc ccatcactac cggttgccag ggtttggact tggtggccga
     7201 atacagccaa agaaaagcaa cgatgttgat gacgtaaaag aacacccagg cgaagcgccc
     7261 gatggagcga aactgcttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttgcggaatt tgccgacgaa
     7441 catgcggtac aacatgagca gaacggccag ccccgccgcc aatccggcat aagccagtcc
     7501 ctcgtagtcg ccctgctcga tgcgaatgac gtgctcgtct atccatttcc agtagggctg
     7561 cgaaaaggag gtgaaaacgc tttcccagat ggagcggtag ttgagaaaac ccgccggttg
     7621 ggaagtgcgg tcggtgatgc cgtcggccat ccaggcaaaa aaaacaatgc cgggtaccac
     7681 tacctgcaaa cctacatgcg ccgcccacag cggtatttgt ttccactcgc ggcgctgcag
     7741 cacccggaaa aagaagtaca gcccgatggt catgcccatg atggcaaagt agtagaaatg
     7801 cagctgcgaa aacaccgcaa tgctgaaggc aatgagtgcg ctgattttcc acgacggctt
     7861 ttcatcaaaa cgcagcagga ggtacagcaa aatgggcagc acttcgggat gtgccagccc
     7921 gaaatgcgaa atcatgcgcc aaagttgcgg cgacaaaaag ctgagcgcca cggctatcgg
     7981 cacgctgtaa acgacgggca gtcccagccg ggcaaaaatg aggtataaaa aaatgccgca
     8041 gagcagcacg cctatgggca tagaccagtt gaaaatgccc agcgccgccc ctgaaatatc
     8101 agtgatgtag cgattgacga aacgaatgcc gttggagaga aaaggctgcg tatcggaggg
     8161 gatgacgtgc tcgccgtagg ggtagttcat gccctgaaac caggtgttgg tgctgtcgtg
     8221 ccggatgtga tacagatagg tggcgtaggc tttgtagccg tcgccgtaag gctcaatgac
     8281 gcgcgtgccg gcgtgctgcg ggaagtcggg caggcgcaag tacagcagca gcgccatgag
     8341 ggctgtggtg aggaggaggc cgaggtaaaa aaaagcagag ttacgcatat cgagagctta
     8401 cactttccaa aacttcaaca acaagacctc cacgcccaga aacaccagcg ccattaccaa
     8461 aaaccagcgc cacagactga taccctgact gcgctcttca attttggccg tcagaatggc
     8521 ttcttcattg atttccagta ctttgatgtt gtctcccaca aaggtttgca actcatcggg
     8581 cacatagcag gctaagtcgg actccttgcg gtcgaaattg aaagcgaatt tgttgatgac
     8641 cgcttcggac tgcaagaaca gatcgtaaaa accagcctgc tgaatctgat tggccgtggt
     8701 gatgaatacc cgcgaaccca caatgcgctg ttcgggaatg aactcgccgg aaggccctcg
     8761 cagcttatat acaatctggt tgccctccac tttgtggtcc gattcgagcg tttccgcccg
     8821 gctgatggtg taggcgatgc gcaggttgcg ggccgatgaa atggcggttt tgtacaacag
     8881 agggataaaa atctcgccgt tgcgcaccaa atcgctttgg gtttcatcca atggcgccac
     8941 gcagaagtac aggtgcccct gatcggcctg gtatttcgac aaaaaagcac tgccgtcgcg
     9001 gaaagctaag agcgtttcgc ccgtgccgct gcgctcgctg cggaaactgc cttttacgga
     9061 gggcaatttc aggttggcgc ttttgttgtc aaacacatcg cggaacacga attccgaggt
     9121 attgatgttg cccacgtttt tcacgccgtc ctcgtaagcc tgcaaagaag cggcgccgaa
     9181 gccctgcaaa aaactgttgt atccggctat gcctgcattg cgcgaaggga acaccagcac
     9241 attgccgccg gcgcgggcgt agcgattgag ttcggcagcc agaccgctcg aaatttcgcg
     9301 cgtttcgttg agtacgatga gttggtaatt gggaaactgc gagaactcca gattccgtac
     9361 cgacatgttc accgctttga aatacgggat gcccgcaaaa gccgcgtcta aatagcggtt
     9421 ggccgattct tcgttgataa ccagcacatt gacctgctcg gccacatgga aggagaagaa
     9481 atatttgtcg tcgaactgca ccgggtagtc ggtcaccgaa agctcggctt cgtgccagcc
     9541 cgtgcgcaat acggtgaggc tcacggtatc ggtagcaacg gccccggcgg ggatggacat
     9601 ggtaccggcg ggcttggttt ggccgtcgta tttgatggca aggcggatgt tttcggcggc
     9661 ctgattgctg taattgcgca cccgcacgat gagcgtattc gtttgcccga tcatttgcac
     9721 cggagcttca aaccaggccg tatctatggc aatgttgcgt tcctgcaccg attgcagcgg
     9781 cacgaggttg atctccggca gggtatcggg ccagtttttg aggtcggtca catttttttg
     9841 gaaatcggag atgagatagg ccgtttccac ttcttccgaa cccgaagcga gggcctgttt
     9901 ctggcgtgtg accacttccg tgaggttgcg caccgagggc gatattttga tttcttcgat
     9961 gagagccaga gcgtcttctt tgctgaccaa gcgctggtgg cggccttcaa agtcgttgct
    10021 cagaatctgg aagcggtcgg tggcggcata agcctccacg atctcgcggg cgcgttgctt
    10081 ggctttttcc accaatggta catcctgact cagggcgctc atactgaagg aattgtctat
    10141 aaaaacagat actgccttgt tgccggcttt tacctctgcg tcctgtggaa tgaagggttg
    10201 cgcgaaagcg aaaatgaggc ccgtcagcgc cagtattctc gacaacagca ccaggagatt
    10261 gcgcagtttg gagcgggcgc tggtttcttc cttcacctcg cgcaaaaagc gcacattggt
    10321 gaaatacacc ttcttgaact tgcgaaagta aaagaggtga atgatgatgg gaatggccag
    10381 cgcggcgagc gcgaacagaa atgtcgggaa taaaaactgc atagttcaga gcctgtcgtt
    10441 acacaatatc ctgccgccgt ccgggcagcc ttctccaaaa cggcaaaggt actgaaatag
    10501 ttggatgcct gtttcatcat acaaccaaaa ctacccctgc ctcgtttgct gtgctgatga
    10561 tttccgccga tgccatagcc gccgtcaatc gcctcgcaga ggaggggcgc cccttttgct
    10621 ttgccttcaa ttttgaagcc gacgaggccc ttatcggcgc cgatgaagac tgggcgcggc
    10681 agggcatttt gttccaaatg ggcgattttt ccaatttcac accgcgcgaa acggaagccg
    10741 cccccgccga ctggctgccg cagcctgtat cttacgagac ctaccgccaa gccttcgagg
    10801 tggtgcatgg gcatttgctg gcgggcaatt cctacttggt caatctcacg tttcccacac
    10861 ccgtacaaac cggcatgagc ctcgaaacca tcttccatcg cagccgggcg ccctacaaaa
    10921 tgctgtggcc gggccgcttc gccgtgttct cgcccgaatc ctttgtccgc atccgcaacg
    10981 gcctcatttc cacctttccc atgaaaggca ccattgacgc cgacctgccc gatgctgcct
    11041 ccaaaatcct ggccgaccga aaagaactgg ccgaacacgt caccatcgtg gacctgctgc
    11101 gcaatgacct caaccgcgtg gccacagacg tgcgcgtgca gcgctttcgc tacttggacc
    11161 gcatcgaaac caatcgcaaa aacctgctcc aggtcagctc cgaaatatcg gggcggctgc
    11221 ccggcgacta ccgcccgcga ctgggcaata tcttggccga actgctgccg gccggctcgg
    11281 tgagcggtgc gcccaaggcc aaaacgctgg aaatcataca ggaagcggaa ggcgctccac
    11341 ggggttttta caccggcgtc atggggcgct tcgacgggca aaacgtagaa agtgcggtga
    11401 tgattcgctt tgtagagcag actgccacgg gtttggtttt tcgcagcggc gggggcatta
    11461 cggtgttcag caagccgcag gatgagcata acgaactgat agacaaggtg tatgtgccga
    11521 ttgcttgaga cgatttgctt catagacggc gcgccgccgc tgttgcccta ccatcaggcg
    11581 cgcttcgatg cggcccgccg gcatttctgg ggggcattgc cgccgctgtt gttgaaagaa
    11641 gtgctcgtcg ttccgcccga tttccgacag agcaaggtca agtgccgcct gacttacagc
    11701 gccgccgatt ttgtggtgga atgggaacac tatacgcccc gccgcatcgg cagcctgcaa
    11761 attgtgtccg gcgaccatct ggactacgcc ttcaaatacg ccgaccgcag cgcgctccac
    11821 gaactgtggc tacaacgcgg cgccgcagac gacatcctca tggtgaaaaa cggcctcatt
    11881 accgatacct cttacagcaa tgtggcgctg tttgacggca cgcgctattt cactcccgca
    11941 caaccgctgt tggccggcgt gcgacgagct gctctgctcg atgccggcc
//
LOCUS       DAORLF010000093        11732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2927_length_11732_cov_2.155006, whole genome shotgun sequence.
ACCESSION   DAORLF010000093 DAORLF010000000
VERSION     DAORLF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_11732_cov_2.155006"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..2264)
                     /gene="pnp"
                     /locus_tag="PK198_09510"
     CDS             complement(18..2264)
                     /gene="pnp"
                     /locus_tag="PK198_09510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRF39015.1"
                     /translation="MQIPISTSFELPDGRQVTIETGKLASQADGSVVVRVGNTMIFAT
                     TVFEKKAREGQSFFPLSVDYQEKFASSGRIPGNFFRRESKLSDYEVLVSRLVDRAIRP
                     LFPDNYMNDTQVIINLISAEKHVKPDAYAALAASAALTLSGSPFEGPISEVRVARIDG
                     EFRINPDKPDLERADIDLIVAATIDNIMMVEGEANECQEADMVAALKFAHEAIKVQCR
                     AQLDLAQKVGGKAIEKAQVEPPVEDLELKARVDALTRQAILDVARGALDKQVRKSRFD
                     EIKEQMIATLTEEKGEEYMETAASMAKNYYDKLKKEVIRRMVMDEGIRLDGRKSTDIR
                     PIWCEVDYLPSAHGSSIFTRGETQSLTSLTLGTKQDEAMIDNSQELYYDKFILHYNFP
                     GFSVGEVKPQRGPGRREVGHANLAARSLRKVLPQENPYTIRIVSDILESNGSSSMATV
                     CAGALALMDGGIQIKAPVSGIAMGLITDGQKFAILSDILGDEDALGDMDFKVTGTENG
                     ITGCQMDIKVDGMPYEILENALLQARDGRLHILGEMKKTIATPREDFKPHAPRIVRIY
                     IEKSYIGAVIGPGGKVVQEIQAETGTVISIEEVEDKGVVSIASSNKDSIERALDRIRR
                     ITFTPSIGDVYDAKVKSVMPYGAFVEFHGKSGLLHVSEMSTQRIENVEDFIKEGDDIR
                     VKLIEIDQKTGKYRLSAKALMEGGESNEPRSGGEHRDGGDRRGGGHHGGGERRGGGGG
                     GHRGSPWE"
     gene            complement(2552..2827)
                     /gene="rpsO"
                     /locus_tag="PK198_09515"
     CDS             complement(2552..2827)
                     /gene="rpsO"
                     /locus_tag="PK198_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRF39016.1"
                     /translation="MAIYLTSEKKADIFREHAGSEKNTGSTEGQVALFTYRIQMLSEH
                     LKKNRQDHACRRSLVALVGKRKRLLKYLMKKDITKYRQLIEKLGIRK"
     gene            2986..>3897
                     /gene="ychF"
                     /locus_tag="PK198_09520"
     CDS             2986..>3897
                     /gene="ychF"
                     /locus_tag="PK198_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRF39017.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNALTSAKALAANYPFATKDPNLGVI
                     TVPDPRLDKLKSLVSPQKVIPTTIEILDIAGLIKGASKGEGLGNQFLANIREVDAIIH
                     VVRCFKDDNVVHVDGDVDPARDKDIIDTELILKDIETAEKRIDRVRKAARVGDKDAQH
                     ELVIGEKILAHLNAGKSARSLPLEDDEKPFLNDMFLLTAKPILYVCNVDEKSVLEGNA
                     YTQAFMQDALKDEPDAEVIFISAEIEAQIAELETMEERMMFLEDMGLHEPGVNRLIRA
                     AYHLLNLITYFTAGEKEVRAWTITKGMT"
     assembly_gap    3898..3980
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4032..4100
                     /locus_tag="PK198_09525"
     CDS             4032..4100
                     /locus_tag="PK198_09525"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRF39018.1"
                     /translation="MKVEGKEYVVEDGDVMHFRFNV"
     assembly_gap    4352..4484
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4623..4698)
                     /locus_tag="PK198_09530"
     tRNA            complement(4623..4698)
                     /locus_tag="PK198_09530"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4663..4665),aa:Gly,seq:gcc)
     assembly_gap    4729..4738
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4834..6672)
                     /locus_tag="PK198_09535"
     CDS             complement(4834..6672)
                     /locus_tag="PK198_09535"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF39019.1"
                     /translation="MNIRHFFICLLLGQALCISGQSTQKPILHGRHWMAITGKPLAAT
                     AGAGIFQQGGNAVDAACAMLAATCTMWDVLSWGGETQVLIYNPKTGKVIAINALGVAP
                     TGATPEFFKGKGMNYPPEFGPLSAVTPGTPGGLLLMLAEYGTMSLEQVLAPAMQLAEG
                     YPIEAQTANAIEKNKAKIKEWPYSKSVMLPHLGEEREAPHPGEIFKQADLLETLRKMV
                     EAEKQALKAKKSRKEAIYAAYDRFYKGDIAREFVRGCQEQGGLITMEDLAKWKVYQEE
                     PLMTTYKGIDVYKLDTWVQGPAMLQALNMLENFDLKGMGYNSSRYIHTLYQVMNMAFA
                     DRDFYYGDPYFPPAEPRRGLMSKEYAKARIRQMLSDKNDPNVGPGDPYPFQGEANPYL
                     DMLKNWNAQPDSASTGYLDGAFRESFLAGTTTVMTADKEGWIVSATPSGGWIPAVIAG
                     RTGVGMSQRLQSFVLDAKENPFNVLEPGKRPRATLTPSLAMKEGKPFLAFAVQGGDTQ
                     DQNLLQFFLNMVEFDMNVQEACEAPNINSYQMRNSFAKHDSRPGVMTVSEELPDWTRR
                     ELMKMGYQLMFERRTSGPINAIFFDRKHGAFQGGSSNHGEDYGIGW"
     gene            7082..8230
                     /gene="folK"
                     /locus_tag="PK198_09540"
     CDS             7082..8230
                     /gene="folK"
                     /locus_tag="PK198_09540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRF39020.1"
                     /translation="MSTTVHTAWLHTGSNMGDRAENLRAALAHIGQSAGRVLQSSSVY
                     QTQAWGLTEQPDFLNQVVQIATTLSPQTLLEAALLIEKSMGRERREKWGSRVIDIDIL
                     FYDDAVLHEENLHIPHPQLHLRNFVLAPLCEIAPDLVHPELNKNIRQLLLESPDSLTT
                     ETYTPARPRPLPYPFIVVEGNIGAGKTTLCQMLARDYGSRLILEQFAENPFLPYFYNN
                     PERYAFPVELFFMTERHKQLQEELQQQSLFEESIIADYFFLKTLLFAKNNLPEEEYRL
                     FQRLFSVLNAAFPKPDLLVFLHRPVESLIHNIKKRGRSYEQDISPDYLRQIQQVYFDF
                     FKSEPPFPIIIIDVENADFQSNHHHYQQILDIIHQEKPTGIVRRMVSL"
     gene            8343..9914
                     /locus_tag="PK198_09545"
     CDS             8343..9914
                     /locus_tag="PK198_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF39021.1"
                     /translation="MPLLLLSFTLQLPAQPAARKKKEAPAVAAQSLPQQIAALDAYIA
                     KARDDWKVPGLAVAIVQGDKVLLSKGYGVLEAGSSNPVDEHSLFAIASNSKAFTSAAL
                     AMLVDEGKLQWDDRVSQYLPWFRLRDPWASADIRVRDLLCHRSGLGTFSGDLLWWGTP
                     YSAREVLERAALLEPAVPFRAKYEYSNLMFIAAGEVIRAVSGLSWEQFTAQRIFSPLG
                     MNRSVTSTRELPAKGNVATPHKTYSDRSQPIEWMNWDVMGAAGGVISSVSDMSKWMQC
                     QLAQGANPPGAKALFTQARSREMWEAHTPIPVSEGSRRRFPSTHFRAYGLGWSLSDYL
                     GYKLVGHGGGYDGMYSQVLMAPEKGLGVVVLTNSMTGIGSAITYKVMDTFLGGAGRDW
                     SSEMLPGAMASQQAFERRIRETITPVATGTHPSQEPSAYTGLFRCPMYGDARVELENG
                     KLVLRLLPYPDLVADLAHLHYDTYEVRWRKDFAWFGSGTAQFIPDSKGRFVRIALDIP
                     NDDLWFYELKLERVE"
     gene            complement(9916..10569)
                     /locus_tag="PK198_09550"
     CDS             complement(9916..10569)
                     /locus_tag="PK198_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF39022.1"
                     /translation="MRLLHIHIAEDQPRLAQMLRDHLELVPDFRVTGVAGNGRVLLDQ
                     LAALPSLPDVVLMDIQMPVLDGIGATEALKAQHPQVKVVMATVFDDEENIFNAILAGA
                     DGYLLKDEAPEVLHRALQEVMSGGAPMSPAIARKSLAMLRRTPVQPQQTDPATELSER
                     ELEILEQLSKGLRYDQVAANLNISTGTVRKHIENIYRKLQVHNKTAAVEAGKKRGWI"
     gene            complement(10656..>11027)
                     /locus_tag="PK198_09555"
     CDS             complement(10656..>11027)
                     /locus_tag="PK198_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39023.1"
                     /translation="PKFMMDKGVYTGVMLGYAEKNFESMIVPVTNDLTGAVRNEVFQP
                     GVKQYSLLVQFGAMPLAKGFGADLYMGFGANYNIFDDGSDIDREAFTIANPLLEHRRD
                     KFFGATIRMGMTLGLNFGRGN"
     assembly_gap    11028..11081
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11082..>11378)
                     /locus_tag="PK198_09560"
     CDS             complement(<11082..>11378)
                     /locus_tag="PK198_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39024.1"
                     /translation="MYRVWLKPNLQAEYLKKAGQCEADRARAAKKAEQAQRRGGSGGF
                     NLFVGANVFPLINTNPKRDYGAVVNFAFPKTALEFSYMIVNQNKENIFDLWIRE"
     assembly_gap    11379..11467
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11468..>11732)
                     /locus_tag="PK198_09565"
     CDS             complement(<11468..>11732)
                     /locus_tag="PK198_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39025.1"
                     /translation="VEYNYPKVLRGMNLLLANVQQNANPTTVNRCAEAAPVAGKYEAK
                     STKVLQLYEAAYRNNYAGTDKSRHQAAAAYAQTTLVIDRQRSEF"
BASE COUNT         2514 a         3286 c         2881 g         2682 t
ORIGIN      
        1 cttcgagcgc cgccatctta ttcccaagga gacccgcgat gtccgccacc gccgcctcca
       61 cgacgctctc cgccgccatg gtgcccgccg ccgcgacgat ctccaccatc acgatgctcg
      121 ccgccgcttc tcggttcgtt gctttcgccg ccttccatca gcgctttggc cgacaggcgg
      181 tatttgcccg ttttctggtc tatttcaatg agtttcaccc ggatgtcatc gccttccttg
      241 atgaaatctt ctacgttttc aatgcgctga gtggacattt ctgaaacgtg caacaggccg
      301 ctcttgccgt ggaattccac aaatgcgcca taaggcatca ccgacttcac cttggcatca
      361 tacacatcgc cgatggacgg cgtgaacgtg atgcggcgaa tgcggtcgag agcgcgctcg
      421 atggagtctt tgttggagct ggcgatgctc actacgccct tgtcttccac ttcctcgatg
      481 ctgataaccg tgcctgtttc tgcctgaatt tcctgtacca ctttgccgcc cgggccgata
      541 acggcgccga tgtagctttt ctcaatgtaa atgcgaacga tgcgtggcgc atgcggcttg
      601 aagtcttcac gcggtgtggc aatcgtcttt ttcatctcgc ccaaaatgtg caagcgcccg
      661 tcgcgcgcct gaagcaacgc gttttcgagg atttcgtaag gcatgccgtc caccttgatg
      721 tccatctggc agccggtgat gccgttttcg gtaccggtca ctttgaagtc catatcgccc
      781 agcgcgtctt catcgccaag aatgtcggaa agaatggcaa atttttgtcc gtcggtaatc
      841 aaacccatag caataccaga caccggcgct ttgatctgaa taccgccgtc catcaaggcc
      901 aaagcgcccg cgcaaacggt agccatagag gaagaaccgt tggactccaa gatgtcggac
      961 acgatgcgga tggtataagg gttctcctgc ggcagtactt tgcgcaaaga gcgggcagcc
     1021 aggttggcgt gtcctacctc ccgacgaccg gggccgcgct gaggcttcac ttcgcccacc
     1081 gagaagccgg ggaagttgta gtgcaggata aacttatcgt agtacaattc ctgcgaattg
     1141 tcaatcatag cctcgtcctg cttggtaccc agcgtgagcg aggtcagcga ctgcgtttcg
     1201 ccgcgcgtaa agatagacga gccgtgtgca gagggcagat agtccacctc gcaccagatc
     1261 gggcggatgt cggtgctctt gcgaccgtcc aagcggatgc cttcgtccat aaccatgcgg
     1321 cgaatgacct ccttcttgag tttgtcgtaa taattcttgg ccatgcttgc ggccgtttcc
     1381 atgtattcct cgcccttttc ttcggtgagg gtggcaatca tttgctcctt gatttcgtca
     1441 aagcgggatt tgcgcacctg cttgtccaaa gcgccgcgag ccacatccaa aatggcctgg
     1501 cgggtgagcg catccacgcg cgctttgagt tcgaggtctt ccaccggcgg ttctacctgc
     1561 gctttttcga tggctttacc acctactttc tgagccagat cgagctgagc gcggcattgc
     1621 actttgatgg cttcgtgagc gaatttcaga gcagccacca tatcggcctc ctgacattcg
     1681 ttggcctcgc cttccaccat catgatgttg tcaatggtag cggctacgat gaggtcaata
     1741 tcggcgcgtt cgagatcggg cttatccggg ttgatgcgga attcgccgtc tatgcgggct
     1801 acgcgcacct ccgaaatcgg gccttcaaag gggctgccgg aaagggtgag tgctgccgac
     1861 gccgccaaag ctgcgtaagc atcgggtttg acgtgctttt cagccgagat caggttgata
     1921 atgacctgag tatcattcat ataattgtcg ggaaagagcg ggcgaatggc acggtccacc
     1981 aaacgggaga cgagtacttc gtaatcggag agtttgcttt cgcgacggaa gaagttgccc
     2041 ggaatgcggc cggaagaagc gaatttttcc tggtaatcca ccgaaagggg gaagaaggac
     2101 tgaccttcgc gggccttttt ttcaaaaaca gtggtggcaa aaatcatggt attgcctacg
     2161 cggacaacca cggagccgtc ggcctgcgag gccaacttgc ctgtttcgat ggtcacctgg
     2221 cggccatcgg gcaactcaaa tgaggtgctg attggaattt gcattcccat aaaaagctga
     2281 ttttaaatga tgtatgtttt aatccttgtt taaccttgac ttgaaatccg gggtaattca
     2341 gggggctgtg caaggttgca gatcagaagg tggaatggga aggggttgat tttcaaaaga
     2401 atggccgaat gtggcccgac taaaaatata acttttccgc ttcgctactg aacgcttttg
     2461 gccgaaaatt cagccttttt tgactcgcca ccgggaaccc ggcataaaac aaaggcagca
     2521 cctcctgtga agcgctgccc tgttttgtct tttacttacg aataccgagc ttctcgatga
     2581 gctggcggta cttggtaatg tcttttttca tcaggtattt cagcaatcgc ttgcgcttgc
     2641 ctaccaatgc caccaaagag cggcggcagg cgtggtcctg acggttcttc ttcaaatgct
     2701 cggaaagcat ctgaatgcgg taggtaaaga gagcaacctg gccctctgtg gagccggtat
     2761 tcttttcaga gccggcatgc tcgcggaaga tgtccgcctt tttttcactc gttaaataaa
     2821 ttgccattgt gaagttttta taccgttagt gatcacatga atcagttaca gcggcgcaaa
     2881 ggtaattttt ttccacatta gcttgtgaga aaaaatttaa gaatctgaca aatgaatacc
     2941 tttgcgccgc tttttaaaaa ccttgatcat caattcgaaa caaatatggg cctgaagtgc
     3001 ggtatcgtcg ggttgcccaa tgtaggcaaa tccacccttt ttaatgcgtt gacctcagct
     3061 aaagcattgg ctgccaacta tccttttgct accaaagacc ccaatctcgg cgtcatcacc
     3121 gttcccgacc cgcgattgga caaactcaaa agcttggtca gtccgcaaaa agtcattcct
     3181 accaccattg agatattgga cattgccggc ctcatcaaag gcgccagcaa aggagaaggt
     3241 ttgggcaatc agttcctcgc caatatccgc gaagtggacg ccatcattca cgttgtacgc
     3301 tgcttcaaag acgacaatgt ggtgcatgtg gatggcgacg tggaccccgc ccgcgataaa
     3361 gacatcattg acacagaact gattctcaaa gacatagaaa ctgccgagaa acgcattgac
     3421 cgcgttcgca aagccgcccg cgtgggcgac aaagacgcgc agcacgaatt ggtcatcggc
     3481 gaaaaaatac tggcacacct caacgccggc aagtcggccc gctcgctgcc tttagaagac
     3541 gacgagaagc ccttcctcaa tgatatgttc ctgctcacgg ccaaacccat cctctatgtg
     3601 tgcaatgtgg acgaaaagtc ggtgctggag ggcaatgcct acacgcaggc tttcatgcag
     3661 gacgccctca aggatgaacc cgatgcggaa gtcattttca tcagcgccga aattgaggcc
     3721 cagatagccg aattggagac gatggaagag cgcatgatgt ttttggagga tatgggtctg
     3781 cacgaaccgg gcgtcaatcg cctcatccgt gcggcttacc acctgctcaa cctcatcact
     3841 tatttcacgg caggcgaaaa agaagttcgt gcatggacta tcaccaaagg tatgacannn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn gccgattacg ttgcgctcgg ctccgaacac gccgtgaaag
     4021 aggccggcaa aatgaaagtg gagggcaaag agtatgtggt ggaagacggc gacgtgatgc
     4081 acttcaggtt taatgtgtag gcacaacgga ggccataaaa acgaaaaggt tcaacagagc
     4141 gactgttgaa cctttttttt gtgcccggag cgggaatcga acccgcactg ccattactgg
     4201 caacaggatt ttcttaccaa ctacaacttt cgttgcccac gcagcaggcg tggtttgtgg
     4261 tctggacttt ctcttcatca tattttcagc atggccgaaa acttagatgc ctcccgtcaa
     4321 gtctctacac ctttcccgcc gcaggcggga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacccgg gcgggacact
     4501 gcgctgcctg cctttgcagg gcaacgctta atgaatacaa acgcaaaaat aagctatttt
     4561 tagctcattc gcgtcacttc aacgaaaaaa gccacccttt ttcaggatgg cttttcttgt
     4621 gttggagcgg acaacgagat tcgaactcgc gacctcaacc ttggcaaggt tgcgctctac
     4681 caactgagct atgtccgcgt ggtttcttaa agcgagcgca aagataaann nnnnnnnnca
     4741 gacgagtttg acaagttttt cagcaacttt ttttcaaaaa aaaatcaccg tccattttaa
     4801 cctgctgaaa atgaaaatct tgcaacaaga tatttaccaa ccaattccat agtcttcccc
     4861 gtgattgctg gagccaccct gaaacgcccc gtgtttccgg tcgaaaaaga tggcgttgat
     4921 cggcccggaa gtgcggcgct caaacatcag ttggtagccc atcttcatca attcccggcg
     4981 cgtccagtcg ggcagctcct ccgaaacagt catcacaccg ggcctcgaat cgtgcttggc
     5041 aaaggaattg cgcatctggt agctattgat gttgggcgcc tcgcaggctt cctgcacatt
     5101 catatcaaac tccaccatgt tcaggaaaaa ctgcagcagg ttttggtctt gcgtatcgcc
     5161 gccctgcacc gcaaaggcca aaaacggctt cccttctttc atggccagac tgggcgtgag
     5221 cgtggccctc ggccgtttgc ccggctccaa tacgttgaag ggattctctt tggcatccag
     5281 cacaaaactt tgcagacgct ggctcatgcc tacgccggtg cggcccgcta tcactgccgg
     5341 aatccagccg ccgctgggcg tagccgatac gatccagccc tctttgtcgg cggtcattac
     5401 ggtggtggtg cccgccagaa atgactcgcg gaaagccccg tccaaatacc ccgtcgaggc
     5461 ggaatcgggc tgcgcattcc agtttttcag catatccaaa tacggattag cctcgccctg
     5521 aaacggatac gggtcgccgg gaccgacatt gggatcgttt ttgtccgaca acatttgccg
     5581 aatccgcgct ttggcatatt ccttcgacat cagcccccga cgcggctcgg caggcggaaa
     5641 atacgggtct ccgtagtaaa aatcgcggtc ggcaaaagcc atattcatca cctgatacaa
     5701 ggtgtggatg tagcgcgagg aattgtaacc catgcccttg aggtcgaagt tttcgagcat
     5761 gttcagtgcc tgcaacatgg ccggcccctg cacccaggta tcgagtttat acacatcaat
     5821 gcctttgtaa gtagtcatca gcggctcctc ctgatacact ttccatttgg ctaagtcttc
     5881 catcgtaatg aggccgccct gctcctgaca gccacgcaca aattcccgcg caatatcgcc
     5941 tttatagaag cggtcatagg ctgcataaat ggcctccttg cgcgattttt tcgctttaag
     6001 tgcctgtttt tcagcctcta ccattttgcg cagcgtttcc agcaaatcgg cctgcttaaa
     6061 aatctccccc ggatgcggcg cctcccgctc ctcgccgagg tggggcaaca tcaccgattt
     6121 cgagtagggc cactccttta ttttggcctt gtttttttca atggcattgg ccgtttgcgc
     6181 ctctatcggg tagccctcgg ccaactgcat cgccggcgcc agcacctgct ccaagctcat
     6241 cgtaccgtac tccgccaaca tcagcaagag gccgcctgga gtgcccggcg tcacggctga
     6301 aagcggtccg aactccggcg ggtagttcat gcctttgcct ttaaaaaact ccggcgtggc
     6361 ccccgtcggc gctacgccca aagcattgat cgcgatgact ttacccgttt tcggattgta
     6421 aatcaacacc tgcgtttcgc caccccaact cagcacatcc cacatggtac aagtggctgc
     6481 cagcatggcg caagccgcgt ccaccgcatt gccgccctgc tgaaaaatgc ccgctccggc
     6541 cgtagccgcc agcggttttc cggtgatggc catccaatgc cggccgtgca gtataggttt
     6601 ctgcgtgctc tgccccgata tgcacagcgc ctgccccagc aacaggcaga taaaaaagtg
     6661 tctgatgttc attttgctct tgtttgggca taaaattaca caaaaatctt gttcttgata
     6721 tgccttttca gggcgtgccg gcgctcatat agaaacatat agcgccgtcg ggtgcctcgg
     6781 tcgtcctcct aacgtcgttc ctcccttcgg ccccctcacg caactggcag agagctgtta
     6841 gctttggctt gtatttatca gggtaaagct aacagttcta caaggaacgg ggccgaaata
     6901 aagtacacat tttcagagag cagatgagtc gtctgaacga gcagaaaaaa gtagccgcag
     6961 tggtgctgcg gcgaggcttt tatgtgaagt gcaggcggct cagatgcctt tgaagcggtg
     7021 taggttattc ggaccccgtt cccaacttca atcccacaat aatttcccta cttttgccgc
     7081 catgtccact actgttcaca ccgcttggct gcacaccgga agcaatatgg gagaccgcgc
     7141 cgaaaacctg cgcgcagcct tggcccacat cggccaatca gcggggcgcg ttctgcaatc
     7201 ctcatcggtg tatcaaaccc aagcctgggg actgaccgag cagcccgatt tcctgaatca
     7261 agtggtgcaa attgccacaa cgctcagccc acagactttg ctggaggccg ccctgctcat
     7321 cgaaaaaagc atgggccgcg aacgccgcga aaaatggggc agccgcgtca ttgatattga
     7381 cattcttttt tacgacgacg cggtcttgca cgaggaaaat ctgcacattc cgcacccgca
     7441 actgcacctg cgcaatttcg tactggcgcc gctctgcgag atcgctcccg acttggtaca
     7501 tcccgaactc aacaaaaaca tccgccaatt gctgctcgaa agtcctgact ccttaaccac
     7561 ggagacatat acgcccgccc ggccccggcc gctcccctac ccttttatcg tagtagaagg
     7621 caatatcggc gccggcaaaa ccaccctctg ccaaatgctg gcccgcgact acggcagccg
     7681 cctcatcctg gagcagttcg cagagaatcc ttttttgcct tatttttata acaaccccga
     7741 gcgctacgcc ttcccggtag agttgttttt tatgaccgag cggcacaagc agttgcagga
     7801 agaactccaa caacaaagct tatttgaaga gtccattata gccgattatt ttttcctcaa
     7861 aacgctgctc tttgccaaaa acaacctgcc cgaagaggaa tacaggcttt ttcagcggct
     7921 gttctcggtg ctcaatgccg ccttccccaa accggatttg ctggtgttcc tgcatcgccc
     7981 ggtagagtcc ttgatacaca acatcaaaaa gcgcggcaga agctacgaac aggacatcag
     8041 ccccgactac ctccgccaga ttcagcaggt ctattttgac ttttttaaaa gcgaaccgcc
     8101 ttttcctatc atcatcatag atgtggaaaa tgctgatttt cagagcaatc atcatcatta
     8161 ccagcaaata ttagacatca tccatcagga aaaaccgacg ggcattgtgc ggcgcatggt
     8221 gagcttgtag atgctgaata gttgccgcca ttaaacacta aaataaacgc attatgatac
     8281 gcatccctct ttccaattgc acaaaggccc gtttcgtact agcctgcgca agatattgcc
     8341 tcttgccgct tcttctgctt tccttcaccc tccaactccc ggctcagcct gccgcccgaa
     8401 agaaaaaaga agcgcccgcc gttgcagcgc aaagcctgcc gcagcagatc gccgcgttgg
     8461 acgcttacat cgccaaagcc cgcgacgact ggaaagtacc cggcctcgcc gtcgccatcg
     8521 tgcagggcga taaagtgctg ctcagcaagg gctacggcgt gttggaagcc ggctcctcca
     8581 atcctgtgga cgagcacagc ctgtttgcca tagcctccaa ttccaaagcc ttcaccagcg
     8641 ctgcattggc catgctggtg gacgaaggca aactccaatg ggatgaccgc gtctcccaat
     8701 acctgccctg gtttcgcctc cgcgatccct gggcttccgc cgacatccgc gtgcgcgacc
     8761 tgctctgcca tcgcagcgga ctcggcacgt tcagcggcga tttgctctgg tggggcacgc
     8821 cctattccgc ccgcgaagtg ctggaacgcg ccgccctgct ggaacccgcc gtccccttcc
     8881 gcgccaaata cgagtactcc aacctcatgt tcatcgccgc cggagaggtc attcgcgccg
     8941 tgagcggcct cagttgggag cagttcacag cccagcgcat tttcagtccg ctgggcatga
     9001 accggagcgt cacctccaca cgggaattgc cggccaaagg caacgtggcc actccgcaca
     9061 aaacctattc cgaccgcagt cagcccattg aatggatgaa ctgggacgtg atgggcgctg
     9121 ccggcggcgt catttccagc gtgtccgaca tgtcgaagtg gatgcaatgc caattagcgc
     9181 agggcgccaa ccctcccggc gccaaagcat tgtttacgca ggcccgcagc cgcgagatgt
     9241 gggaagcgca cacgccgatt cccgtatcgg aaggttctcg gcggcggttt cccagcacac
     9301 attttcgggc gtatgggctt ggctggtcgc tgtccgacta tctgggctac aaattggtgg
     9361 ggcacggcgg cggctacgac ggcatgtact cgcaggtgct catggcgccc gaaaaaggac
     9421 ttggcgtggt ggtcctcacc aacagcatga caggtatagg atcggccatt acttataagg
     9481 tgatggatac ttttctgggg ggcgccgggc gcgactggag cagcgaaatg ttgccgggcg
     9541 ctatggccag tcaacaggcg tttgagcggc gcattcggga aaccataaca ccggttgcca
     9601 caggtacgca ccccagccaa gagccgtccg catataccgg cttgttccgc tgcccgatgt
     9661 acggcgatgc ccgcgtggag ttggaaaatg gaaaattggt gctgcgattg ttgccttacc
     9721 ccgatctcgt ggccgatctc gcgcatttgc actacgatac ctacgaagtg cgctggcgca
     9781 aggattttgc ctggtttgga agcggtaccg cacagtttat tcctgattcc aaaggccgtt
     9841 ttgtccgtat tgctctggac attcccaacg acgacctctg gttttatgaa ctcaagctgg
     9901 agcgcgtgga gtagatcaaa tccagccccg ttttttgccg gcttctacgg ctgcggtttt
     9961 gttgtgtacc tgcaatttgc ggtagatgtt ttctatgtgt ttgcgcaccg ttcccgtact
    10021 gatgttcaga ttggccgcca cctgatcgta gcgcagcccc ttgctgagtt gttccagaat
    10081 ttccaattcg cgttcgctca gttcggtcgc agggtccgtt tgttggggtt gcacaggagt
    10141 gcggcgcagc atggccaatg atttgcgcgc tatggccggc gacatgggcg cgccgccgct
    10201 catcacttcc tgcaaagccc ggtgcagcac ttcgggcgcc tcgtctttga gcagatagcc
    10261 gtcggcgccc gctaatatgg cattgaaaat attttcctcg tcgtcaaaaa ccgtagccat
    10321 caccaccttc acctgcgggt gctgcgcctt gagcgcctcc gtagcgccga tgccgtccag
    10381 cacgggcatt tgtatatcca tcagcaccac gtcgggcagg ctcggcaatg ccgccaattg
    10441 gtcgagcaat acccggccgt ttcccgccac gcccgtcacc cgaaaatcag gtacaagctc
    10501 cagatgatcc cgcagcattt gcgccaaacg cggctgatct tcagcaatat gtatatgcag
    10561 cagtcgcaat gctattgatt ttttattgaa tcgaactctt tcacccgcca aagccttggc
    10621 gtcggcggct cacccactca cccactcacc cctgctcaat tccctctccc aaaattcagt
    10681 cccagcgtca tccccatgcg aatggtagcc ccgaagaatt tgtcccttct gtgttccaaa
    10741 agcggattgg cgatggtaaa agcctcccgg tctatatcgc tgccgtcgtc aaaaatgttg
    10801 tagttggccc cgaatcccat gtagaggtcc gctccgaatc ccttggccaa aggcatggct
    10861 ccaaactgca ccaacaacga atactgtttt acaccgggct gaaatacctc gttgcgcacc
    10921 gcgccggtca gatcattggt caccggtacg atcatggatt caaaattctt ctccgcatac
    10981 cccagcatca cgcccgtgta aacacctttg tccatcataa atttgggnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttctcgta tccaaaggtc
    11101 aaaaatattt tctttattct gatttacaat catatacgaa aactccagcg ccgttttcgg
    11161 gaaggcaaaa ttcaccacag caccgtagtc tcttttcgga ttggtattga tgagcggaaa
    11221 cacattagcg cccacaaaga gattaaatcc gccgctgccg cctcggcgtt gcgcctgctc
    11281 cgccttcttg gctgcccgtg cgcgatctgc ctcacactgt ccggcctttt tcaggtattc
    11341 ggcctgcagg ttgggcttca accacacgcg gtacatttnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnncaa actccgagcg ctgacggtct atcaccagcg tagtctgggc ataagctgcc
    11521 gccgcctgat gccggctttt gtcggtaccg gcgtagttat tgcggtaagc agcctcgtac
    11581 aactggagca ctttggtact ttttgcttcg tatttgccgg ctaccggagc ggcttccgca
    11641 caccgattca ccgtcgtcgg attggcattt tgttgcacat tcgccagcag caggttcatg
    11701 ccgcgcagca ccttggggta gttgtattcg ac
//
LOCUS       DAORLF010000094        11696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2935_length_11696_cov_1.705609, whole genome shotgun sequence.
ACCESSION   DAORLF010000094 DAORLF010000000
VERSION     DAORLF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2935_length_11696_cov_1.705609"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>525
                     /locus_tag="PK198_09570"
     CDS             <1..>525
                     /locus_tag="PK198_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF39026.1"
                     /translation="ILIAAVVVVAGLGWMSSQNTYLDFAPQYEKTISHTRFDNLLEAT
                     AQGEDISTMERFYRWIAGFYMVGERPLTGFGPGNFLEQYKAYTVSSFRTYVSHNPEQS
                     GIHCYYLMISVEQGLPGLLFFLLMSFAALVYGEQLYHRVRDRQTRQAVMATMLSFISI
                     LILCVINDLIETDKI"
     assembly_gap    526..658
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <659..1499
                     /locus_tag="PK198_09575"
     CDS             <659..1499
                     /locus_tag="PK198_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF39027.1"
                     /translation="AESSKWARLLHRPFRYLSAIAFRHLLYPLLKRPWNRQCLTFFGV
                     PMGVQLPAGMDLFLLGCKTHDSELRLARFFIRFLQKGDYVADVGAHFGYFSLLAAHLV
                     GPEGRIWSFEPGSATHAALRNNAQVFRQIEAHRLLVGTIDGSCDFWEFPTLFSEYNTT
                     RPDSVPDHLQGRKTTLTSTRLDTFFAACQAIPRFIKIDVEGAEHEVLQGLKGMFLQGV
                     FPVVAMEFLGGPGHRQAVQWMLEHGYRVFVPDAAGELQACEHWDTWLADRKTDSDNFV
                     FVR"
     gene            complement(1672..>1883)
                     /locus_tag="PK198_09580"
     CDS             complement(1672..>1883)
                     /locus_tag="PK198_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39028.1"
                     /translation="ALLILLPFPRWQKAALIYMVLWGLLTTLARWAAYVHFDLPWWPQ
                     TFLAWTPEVLIRTPHFLIPLALLRR"
     assembly_gap    1884..2035
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2036..2520)
                     /locus_tag="PK198_09585"
     CDS             complement(<2036..2520)
                     /locus_tag="PK198_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39029.1"
                     /translation="MRTLSRQHSFLFLLQTAAFLTLAGYAWLSWTGQSPLSVLFWNEQ
                     WLRGAVGSLSGMSWEAYLASPATSRFWSGVEWGMGLALSLAAVSAWIAQAKPRLAAPF
                     LLFGFLLVLLFSILYGFDKIFFWVQMPEYALQWGAPLFLAVWLSAKIQTRVVWGLRIV
                     AA"
     assembly_gap    2661..2853
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2854..4211
                     /locus_tag="PK198_09590"
     CDS             <2854..4211
                     /locus_tag="PK198_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRF39030.1"
                     /translation="VLAMAVLMIVWWVTEAVPIPVTSLLPIVCLPLLGVGSVKESAAP
                     YSNPVVFLFMGGFMVALAMEKWNLHRRIALNIIRLTGTNANGILLGFMLATGFLSMWI
                     SNTATAVMMLPIAVSVIDLLTKNMGERNDPRIRNFALAMMLGIAYCASIGGMATIIGK
                     PPNVVLKGFVRDQYDYEIGFGQWMLAGFPFAMLLLFITYFILVKILYPNKLGRFEGGR
                     ELIRQELNELGRMSKGEVGALAVFAGTALLWMFGGLLNKAIPGLKLSDEGIALMGATT
                     LFLYPLDFGKGKFLLDWRDTQHLPWGILLLFGGGLSLADALAKTGIIGLIGHQFAGLE
                     SVGFLLILGLTAVSLYLTEVMSNVALVTIFLPVVGGVAAGAGIDPILVLMPVTLAASC
                     AFMLPMSTPPNAIVFASGHIKIAQMVRAGFWLNIIAILLIAALVEPMVQLIFSDWLGG
                     K"
     assembly_gap    4275..4307
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4585..5337
                     /locus_tag="PK198_09595"
     CDS             4585..5337
                     /locus_tag="PK198_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF39031.1"
                     /translation="MPTETTSISILIVEDDASFALELEMLIQDIGYQVAGVADSAASA
                     LEEIYSKRVDFILMDVDIKGKMTGLELGKKIRPIKIPILYITSFGTKEHYEEAQKSNI
                     VGYLVKPIDKYTLQTAISLAVGQLFAALKDGKQLSADEFLFEEYLFLKKDSVYHKVAE
                     RDIMLVEGSDDYVKIRLFDGRSFILRKTLQIMERALSEALFIRVHRSYLINVTAIEQL
                     DTQDNLLLVGDTAIPLSRKRRLELEKLIRKVE"
     gene            5465..>6557
                     /locus_tag="PK198_09600"
     CDS             5465..>6557
                     /locus_tag="PK198_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39032.1"
                     /translation="MEKLADKARTQQERLDAMKTLIDSSYVVDLARTATYVEQMRDMA
                     RRAGSDYHLGFVEEKQGMLSYRGSRLDSARVQYNRSYEYYQKAGRQEEALLAYTRIGI
                     MYSIEGRFADAARIYANVKARAGGMTKVAGFLYNQLGTLYTYKANPDSAGYYYTQAVE
                     QYKLLNDTVGYLRPMYNHAWMLATNSREKEAKTMAYTVLELQERMKAWEDASYTYGLL
                     VNLLVGEGDYENALIYAEKNYNYVKIEKNSRKMMGALAALAQIKSASDNPAASIPYLE
                     EALTYAEKSNEREAKEIALLELGQTYLAMRKFDKAKEYLTACLALTEDKRESYLAPTA
                     MAHLGQAYYQTDQLEEAKQWLYQALDLLEQ"
     assembly_gap    6558..6593
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6675..>7336
                     /locus_tag="PK198_09605"
     CDS             6675..>7336
                     /locus_tag="PK198_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39033.1"
                     /translation="MAQEMGDNKTWFLEMTSTLYQAHKDVGDYKAALQYHEQYKQLSD
                     TLYNVAKVKAEAKQLKDFEFKLEKETITLEQQAREDKLKAKARQNALIAGFIGLLALV
                     SFAFFANLRRQQARIRAQNEALTNLNATKDRIFAIIGHDMRKPALAFRGMTQKINYLL
                     QKQDYARLTALGNQLEESAYSLNTVTDNLLNWALLQKNIVGYQPQSVVLQQVAEELQS
                     IFA"
     assembly_gap    7337..7434
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7435..7743
                     /locus_tag="PK198_09610"
     CDS             <7435..7743
                     /locus_tag="PK198_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39034.1"
                     /translation="SIKYTPEGGVVNVTATSDGEWVNIGVQDSGIGIPEAEQKNLFML
                     RNDKSREGTAGEKGTGLGLHLAHELAKINKGALALNTRIAQGTSFELRLPRAAEAPAQ
                     "
     gene            8457..>11696
                     /locus_tag="PK198_09615"
     CDS             8457..>11696
                     /locus_tag="PK198_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39035.1"
                     /translation="MEKVLLLLRPNPSASADGRLPPKHQGRARHKVLSLVQSGGPKEA
                     LRPNGKVFNAYFLIQQFASNMKNFFSRPSKATTLAWFFLAFFAHFSVYLQAQSRTEYP
                     VPVNNVVPGGSITTAAGKPSPTSPVPPIQMNGDSRSTTLPAQNFSMKSTACSGTANIP
                     PNGNLVVNGINVSSVSTGDVNSYTFPFSSCGFATSPGSLWLGSGDASFSTTLTFSTPV
                     NSLVIIINGSNTGERFLFTSTNSLTVTDLGSCGYTVVGQEAICISDGIPTEGSGGAFQ
                     ISAAAPYTQLTISGIGSQNGSLLAACTTTIAPPTPTCTGVTGGTGPDDDFDGDGICNK
                     DDLDSDNDGILDTNECGTSAGSGATAGSITEIISQQQYVQVLDGLNITYTATAGTVSP
                     FTENVGGSNTLYATGTPISYLSPNPGSTIRMDFSTPTKIAFGITDIDQANENYMITVY
                     DENNNVVPNPGTYVSSMFAGTSGTYPNGVPANYNDLGTNLSIISNPTSVTLVSGSVND
                     ATFVRANSYVLDFTAAPFEVSRIDISHIGTTGSPAILIANIGGGGVGNCPDTDGDNFT
                     NRFDTDSDGDGCFDAIEGGGNFTSVNVNANGMLTGGVDANGVPIAATASGQSIGSSQN
                     NAVQGTDCCPSLANTPGNVVINNGICGPSCQPVGASISAPIIGCPGGSSIQYSVNNGA
                     WTTTLPAYADGLTIRTRCVCDSSPSTVSPVSSGATTNATNCLDNTPPTAICSNASVTF
                     NGQESIALNANTLVTANDNCGIQSIMLSTSSITCQQLGQTVPVTVTVRDLSNNTATCT
                     SNITVGGLPCGWSQNPDCVGCTNGNSVSYNPPSGVFTVTSVNCFYGPSFTHDSKAFAQ
                     RTLCGNGSITALVTDISSISTGWAGIVMRENKDPNAKKAQLMTNLSNLSRREFRTATN
                     GQAYPQQFPSQNRYWLRITRTGNQFVMYVSPNGTSWFVVGSQNIVMPSCILMGLVVTN
                     YTPVSTVTASFSNVSFTGGNSGLGSPFAPDVFTPLHSPLTPDFDVFPNPTSGDLNVNL
                     SQYIGRSVRIETYSLEGKLLQFTELDEVQHTPERLDMKGLQTGMYLVKVK"
BASE COUNT         2729 a         2795 c         2963 g         2564 t
ORIGIN      
        1 attttgattg cggcagtggt agtagtggcc ggcttgggtt ggatgagttc acaaaacacc
       61 tatttagact ttgccccgca atacgaaaaa accatcagcc atacccgctt cgacaacctg
      121 ttggaggcca ctgcgcaggg cgaagatatt tccactatgg agcgttttta ccgctggatt
      181 gcaggctttt acatggtggg cgaacggccg ctgacgggat tcgggccggg caattttctg
      241 gagcaataca aagcatatac cgtttcgagt tttcgcactt atgtgagcca caatcccgaa
      301 caatcgggca tacactgcta ctatctgatg atttctgtgg agcaagggct gcccggactg
      361 ctgtttttcc tgctgatgtc ttttgccgcg ctggtctatg gcgagcaact gtaccacagg
      421 gttcgggacc ggcaaacccg gcaggcggtg atggcaacca tgctgtcgtt tatctccatt
      481 cttatacttt gcgtcatcaa cgatttgatt gaaaccgaca aaatcnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
      661 cagaaagctc caagtgggcg cgcctgttgc accgcccgtt tcgctatctg agcgccattg
      721 cttttcgcca ccttctttat ccgctgctca aacggccctg gaaccggcaa tgcctcacct
      781 ttttcggagt cccgatgggg gtacaattgc ctgccgggat ggatttgttt ttgctgggct
      841 gcaaaaccca tgattccgaa ttgaggctgg ctcgtttttt tatacgcttc cttcaaaaag
      901 gcgattacgt tgcggatgtg ggggcgcatt ttggttattt ttctttgctg gcggctcatc
      961 tcgtcgggcc ggaggggcgc atttggtcgt ttgaacccgg cagcgctacc catgcggcac
     1021 tccgaaacaa tgcacaggtt ttcaggcaga tagaagcgca tcggctgttg gtgggcacca
     1081 ttgacggctc ctgcgatttc tgggaatttc ccacgctttt ttcggaatac aatacgaccc
     1141 gccccgattc agtgcccgat catttgcaag gtcgaaaaac tacattgacc tctacccgtt
     1201 tggacacgtt tttcgcagcg tgtcaagcca ttccccgttt tatcaaaata gacgtggaag
     1261 gcgcagagca cgaggtattg caggggctga aaggcatgtt tcttcaaggg gtttttcctg
     1321 tggttgcgat ggaatttctc ggcggtccgg ggcatcgtca ggcggtgcaa tggatgctgg
     1381 agcatggcta tcgggtgttc gtgcctgatg ctgccggcga gctacaagct tgtgaacatt
     1441 gggatacctg gcttgccgac cgcaaaacgg attctgataa ttttgtattt gtgaggtaaa
     1501 aattcctgcc cgctctcccc taccctgttc agctatcaag attcgccaag cgaggtctat
     1561 tccatatctg cctgggcagt ttttttgaaa caacaacact ttctggctgg aaaattttgt
     1621 ctgagtaagt gaacctgatg ctatgccgag aaaacaacct gcagggcgtt atcaacggcg
     1681 gagcaaagcc agcggaatga ggaaatgagg cgttcggatc agtacttcgg gggtccaggc
     1741 caagaaggtt tggggccacc agggcaggtc aaaatgtaca tacgccgccc atcgagcgag
     1801 cgtggtgagc aatccccaga gcaccatata gatgagcgca gctttttgcc atcggggaaa
     1861 tgggagcaaa atcaataagg ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggc
     2041 cactatgcgc aggccccaca caacacgcgt ttggattttc gccgacagcc agactgccaa
     2101 aaacagcggg gcgccccatt gcagggcata ttcgggcatc tgcacccaaa aaaatatttt
     2161 gtcgaaaccg tagaggatgg agaacaacaa cacaaggaga aagccgaaga gcagaaaagg
     2221 agccgccagt cggggcttgg cttgggcgat ccatgcggac acagccgcca gtgagagggc
     2281 caggcccatc ccccactcta ccccgctcca aaaacgggat gtggccggag atgcgagata
     2341 ggcttcccag gacataccgg acaagctgcc cactgcgcca cgcagccatt gttcgttcca
     2401 aaacagtacg gacagcggcg attggccggt ccaggatagc caggcgtatc cggctaaggt
     2461 gaggaaagcg gcggtttgca gcaggaacag gaagctgtgt tggcgtgata gcgtgcgcat
     2521 gatgtgcaaa gaacgcagga gaggaaaatg tggttgtgtt tagtgaggcc atgcgccagg
     2581 ggcagtagcg gtaccggctg cgggggagat agagggagag cagcagccgg taagagcgaa
     2641 tgacccgacg gcccgcctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagtgct ggcgatggcc gtgctgatga
     2881 ttgtatggtg ggtgacggag gcggtgccga ttccggtgac ctcgctgctg cccattgttt
     2941 gcctgccgtt gctgggagtg ggcagtgtga aggaatctgc ggcgccgtat tccaacccgg
     3001 tggtgttttt gttcatgggc ggttttatgg tggcgctggc gatggagaag tggaacctgc
     3061 accggcgcat tgccttgaat atcatacgat tgacgggaac gaatgccaat ggtattttgc
     3121 tgggttttat gctggccacc ggctttttga gcatgtggat cagcaatacg gctacggcgg
     3181 tgatgatgct gcccattgcg gtatcggtga ttgatttgct gacgaaaaat atgggggagc
     3241 gcaacgaccc gcgcattcgc aattttgcat tggccatgat gctgggcata gcttattgtg
     3301 ccagcatagg cgggatggct accatcatag gtaagccgcc caatgtggtg ctgaagggct
     3361 ttgtgcgcga ccagtacgat tacgaaatag ggtttgggca gtggatgctg gcgggctttc
     3421 cgtttgcgat gctgttgttg tttatcactt attttatatt ggtcaaaata ttgtacccca
     3481 ataaattagg caggtttgaa ggcgggcggg agctcatccg gcaggaactg aatgaattgg
     3541 gccggatgag caaaggcgaa gtgggcgcat tggcggtctt tgcgggtacg gcgctgctgt
     3601 ggatgttcgg cggcctgctg aacaaggcca ttcccggtct gaaactgtcg gacgaaggca
     3661 tcgccctgat gggggcaacg acgctgtttt tgtacccact tgatttcgga aaaggaaaat
     3721 ttttgttgga ctggcgcgat acgcagcacc tgccctgggg cattttgctg ttgttcggcg
     3781 gcgggctgag cttggcagat gcgctggcca aaacgggcat catcgggctg atcggccatc
     3841 agtttgctgg tttggaaagc gttggttttc tgctgattct ggggctgacg gcagtgtcgt
     3901 tgtacctcac ggaggtgatg tcgaatgtgg ctttggtgac gatctttttg ccggtggtgg
     3961 gcggcgtggc ggcgggcgcg ggcattgatc cgattttggt gctgatgccg gtgacgctgg
     4021 cggcgagttg tgcttttatg ctgccgatgt ccactccgcc gaatgctatt gtgtttgcga
     4081 gcggacatat caaaatagcg cagatggtgc gagctgggtt ttggctcaat atcattgcca
     4141 tattattgat tgcggccttg gtggagccga tggtgcagtt gattttttcg gattggctgg
     4201 gagggaagtg agcggtaggc ggtagccgcg ccgtggcgcg gtaagcgcga gggaacgcga
     4261 aatttttgaa cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga atgcgagggg
     4321 aaatccgaaa ccttgaatcc gaaacaactc tcctaccact caccgcagga ttcctaccat
     4381 tcaccgcatt atcttgtatg ttcaccgcaa aaaattttaa aaactacacg acacgagtat
     4441 gagcttgtag ctttgtagcg ttccgtcagc gcttgatttt ttaatatttt tgctcctaaa
     4501 tgatgaacaa actatccgat aagcgattac ttttggggca aagcaataaa atgcctccct
     4561 tactaacatg agtctttttc ctccatgcct acagaaacta catccatatc catacttatt
     4621 gtagaggacg atgcctcctt tgcattagag ctggaaatgt tgatacaaga catcggctat
     4681 caggtagccg gagtagcgga cagcgccgca tcagcattgg aagagattta ctccaagcgg
     4741 gtagatttca ttctgatgga tgttgacata aaaggtaaaa tgaccggatt agagctgggc
     4801 aagaagatac gtccgataaa aattcccatc ctgtacatca ccagttttgg cactaaggag
     4861 cactacgagg aggcccaaaa atcgaatatt gtgggttacc tcgtcaagcc tattgacaaa
     4921 tacaccttgc agacggccat ttctttggca gtcggacagt tgtttgccgc tctgaaggac
     4981 ggcaaacaac tgtccgccga tgagttcctg tttgaggaat acctgttttt aaaaaaagac
     5041 agtgtgtatc ataaagtagc cgagcgcgat attatgctgg tggaaggctc ggacgactat
     5101 gtaaaaatcc gcctcttcga cgggcgctcg ttcatactgc gcaagacatt gcagatcatg
     5161 gagcgcgccc tgtcggaggc cctctttatc cgcgtacacc gcagctacct gataaacgtg
     5221 accgccatag aacaattaga tacccaagac aacttgcttt tggtgggcga cacagcgata
     5281 ccgctgagca ggaaacgaag gctggaattg gaaaaactga tcagaaaagt agaataaccc
     5341 gcatccaatt ttgtacttca tcttcaatcc tataatcatg tctaacaatt atacccgccg
     5401 actttggctc cttggtaccg cttggctgct gtgtaacata ctgcacgctc aagacttaga
     5461 gcgcttggaa aaattggcgg acaaagcccg cacgcagcaa gagcgtttgg atgccatgaa
     5521 aacgctcatt gattccagtt atgtggtgga tttggcccgc accgccactt atgtggagca
     5581 gatgcgcgat atggcccgac gcgccggatc ggactatcac ctgggttttg tggaggaaaa
     5641 acaggggatg ctgtcttacc gaggctcccg tttggattcg gccagggtgc agtataaccg
     5701 ctcctatgag tactaccaaa aagcagggcg ccaggaggag gccttgttgg cctatacgcg
     5761 cattggcatc atgtactcga tagaggggcg ctttgctgat gcggccagaa tatacgccaa
     5821 cgtgaaggcg cgggctggag ggatgaccaa ggtggccggt tttttgtaca accagttggg
     5881 gacgctatat acatacaaag ccaatcccga ttcagcaggg tactactata cccaagcagt
     5941 tgagcaatat aagttgttaa atgacacggt ggggtacctg cgccctatgt ataaccatgc
     6001 atggatgtta gctaccaaca gcagggaaaa agaagccaaa acgatggctt ataccgtcct
     6061 agaactgcaa gaacgaatga aggcctggga ggatgccagc tatacctacg ggctgttggt
     6121 taatctgttg gtgggtgaag gggactatga aaacgcgctg atttatgcgg aaaaaaacta
     6181 caactacgtt aaaattgaaa aaaacagccg caaaatgatg ggggcgctgg cggcgttggc
     6241 gcaaataaaa agcgcaagtg acaacccggc tgcgagtatc ccttatctgg aagaagccct
     6301 aacttatgca gaaaagtcga acgagcggga agccaaggag atagctttgc tggaactggg
     6361 gcaaacctac ctcgccatga ggaaatttga caaagcaaaa gaatatttga ccgcctgtct
     6421 tgccctgacg gaagataagc gcgaaagcta tcttgcacca acagccatgg cgcatttagg
     6481 gcaagcctac taccagactg accagttgga agaagccaaa caatggctat atcaggcttt
     6541 agacctcttg gaacaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtttatg
     6601 gtattctcgc cagtgtccat ctgacggaaa agcagccaag gttggctatt caaatggcgg
     6661 aaaaagcata cggcatggcg caagaaatgg gcgacaacaa aacatggttc ctggagatga
     6721 ccagcaccct gtaccaagcc cacaaagatg ttggagacta caaagccgcc ctccaatacc
     6781 acgaacaata caagcaactg agcgacaccc tctacaatgt agcgaaagtg aaagcagagg
     6841 ccaaacaact caaagacttc gagtttaaat tggagaagga aaccatcacc ttagagcaac
     6901 aagccagaga agacaaactc aaagccaagg cccggcaaaa cgccctgata gcgggcttca
     6961 tcggcttgct ggctttggtc agttttgcct tttttgccaa cctgcgcagg cagcaagccc
     7021 gcatccgcgc ccaaaacgag gcgctgacga acctcaacgc caccaaagac cgcatctttg
     7081 ccatcatcgg ccacgatatg cgcaaacccg ccctggcttt ccggggcatg acccaaaaga
     7141 tcaactacct gctccagaaa caggactacg cccgcctcac cgctctgggc aatcaattgg
     7201 aagaaagcgc ctactcgctg aacaccgtca ccgacaacct cctcaactgg gcattgctgc
     7261 aaaaaaacat cgtgggctac cagcctcaat cggtggtatt gcaacaggtg gccgaggaac
     7321 tccagtccat ttttgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccatc
     7441 aaatacacac ccgaaggcgg cgtcgtcaat gttacagcta cctcggacgg cgagtgggtc
     7501 aacatcggcg ttcaggactc cggcatcggc attcccgaag cagagcagaa aaacctcttc
     7561 atgctacgga acgacaaaag ccgcgaaggc accgccggcg aaaaaggcac cggcctgggg
     7621 ctgcatctgg ctcacgagtt ggccaaaatc aacaagggcg ctctggccct gaacacgcgc
     7681 atagcacagg gtacgagctt tgagttgagg ttgccgcgag cggccgaggc gccggctcag
     7741 tgaaggcggg acgagtgggc ggtaggcggt gggcgcgccg tggcgcgata agcgcgagaa
     7801 agcccgaaac tttgaacccg ctttatttcc gacgctgatc tttatgattt ttatgattgg
     7861 cgctgctcgg tgagggacat aacgatcata ataagctgtg tcttccagcg tcgaaaaaac
     7921 cgcaagccgc ccgttcctgc cggaacgcca aaatctcttt aacgcgaatc aacgcgaaac
     7981 acgcgaaatc ccgcgaacgc ggaaccagta ggcggtcggc ggtgcgcggc ttgcagcttg
     8041 tagccagcag cttgaggccg ttcccacccg aaattcccat gcacttcacc gcaggatttt
     8101 gtcatctcac cgcgcttttc ctaccattca cctcaaagtt gccaaaaaat ttggcgatag
     8161 ggatgcggcg accataactt tacagggatt ttcagcggtt tttagggctg tctgtttcat
     8221 gttttatcac ctcagacgat tgggttttta ccgcagaacc gcctaaaaaa acggttccat
     8281 cacggtacac agcaaggcat ctttcaaagg tggcgcaggc atcgccgtgt ctttatgtaa
     8341 aggtggccaa aatgtgtcgg cggtatggtt ttatcggtct gaggcagcct tgaaagcgct
     8401 acaacaatac gcaacttaac aaatcattca tttattcacc aaacattaca tttctcatgg
     8461 aaaaagtatt gcttttatta cggcctaacc catcggcaag tgccgacggg cgtctgcccc
     8521 ccaaacatca gggccgtgcg cggcacaagg tcctgtcctt agtccaaagc ggcggcccga
     8581 aagaggccct acggcccaac ggcaaggttt tcaatgctta ttttttaatt caacaattcg
     8641 cttctaacat gaaaaacttc ttctctcggc cctcaaaggc caccacattg gcctggtttt
     8701 tcctggcatt ttttgctcat ttttctgtct atctgcaagc ccagtcccgt actgagtatc
     8761 ccgttccggt caacaatgtc gtaccgggcg gcagcataac gacggctgcg ggtaagcctt
     8821 ctccgacgag cccggtgccg cccattcaga tgaatggcga tagccggagt acaacgctgc
     8881 ctgcccaaaa cttcagcatg aaatctacag catgttccgg cacagcaaat attccgccta
     8941 atggaaatct tgtagtaaat ggtataaatg tgagctctgt atccaccggt gatgttaatt
     9001 cttatacttt tccgtttagt agttgtggtt tcgctaccag tccaggatct ttatggttgg
     9061 gtagcggtga tgcgtctttt tcaacaacgc taaccttttc tacgccggta aatagtttgg
     9121 ttattattat aaatggatca aatacaggtg agcgtttttt attcacaagt acaaattctt
     9181 taactgttac ggatttagga tcttgtggtt atactgtggt cggacaagaa gccatatgta
     9241 tttctgatgg tattcccact gaaggtagcg gtggggcatt ccaaatttcg gcagcagctc
     9301 cttacactca attaacaata tctggtattg gatctcaaaa tggatcattg cttgctgcat
     9361 gtacgacaac tattgcgcct cctacaccaa cctgcacagg cgtgacaggc ggtaccgggc
     9421 cggatgatga ctttgacggc gatggtattt gcaacaaaga tgatctggat agcgacaatg
     9481 acggcatctt ggatacaaat gagtgcggaa catctgcagg gtcgggagcg acggcgggaa
     9541 gtatcacaga aataatatcc cagcagcagt atgtccaggt acttgacgga ctcaatatta
     9601 cctacaccgc tacggccggg actgtgagtc cttttacgga aaatgtcggt ggttcaaata
     9661 cgctgtatgc aacaggaacc cctatctcct atctgtcgcc caatccgggt tcgacgataa
     9721 ggatggattt cagcacgcct accaaaatag catttggtat aacagatata gatcaggcca
     9781 acgagaacta tatgattacg gtatatgatg agaacaacaa cgtagtgcct aaccccggaa
     9841 cctatgtttc ttccatgttc gcgggaacat cgggtactta ccccaatgga gtacccgcaa
     9901 attacaatga tttgggtacc aatttgagca tcatttccaa tcctacgagc gtaacccttg
     9961 tatcgggttc agtcaatgat gcaacattcg ttcgggccaa ttcctatgta ctggatttca
    10021 ccgcagcccc ttttgaggtt tcaagaattg acatatcgca tattggaact acagggtctc
    10081 ctgcgatatt gatcgccaac attggcggcg gcggagtggg caactgccct gatacggatg
    10141 gagataactt cacgaaccga tttgacacag actccgacgg cgacggctgt ttcgatgcca
    10201 tcgaaggcgg cggcaacttt acatctgtca atgtaaatgc caacggcatg ttgaccggcg
    10261 gagtagatgc caacggcgtc cccatcgcag ccactgcaag cggccagtct attggaagca
    10321 gccaaaacaa tgcggtacag ggcaccgatt gctgccccag cctcgccaat acgccgggca
    10381 atgtagttat caacaacggc atctgtggcc caagctgcca gccggtaggc gcaagcatct
    10441 ctgcgccgat aattggctgc cccggcggta gctccataca gtactcggtg aacaatggcg
    10501 cctggacgac caccctgccg gcatatgccg acggcctgac catcaggact cgctgcgtat
    10561 gcgacagcag tccttcaacc gtcagcccgg tatcatcagg cgcgaccacc aatgcgacca
    10621 attgcttgga caatacgccg cccacggcca tctgctccaa cgcttcggtt accttcaatg
    10681 gccaagagag catcgcattg aatgccaata ctttagtgac tgctaacgat aactgcggta
    10741 ttcagagcat catgctgagt acttcgagca tcacctgcca gcagttgggc caaacggtac
    10801 ccgttaccgt taccgtaaga gacctgagca acaatacagc tacttgtacc tccaacatca
    10861 cagtaggcgg cctgccctgc ggctggagcc aaaaccctga ttgcgtgggc tgtaccaacg
    10921 gcaacagcgt ttcctacaat ccgcccagcg gcgttttcac ggttacgagc gtaaactgct
    10981 tctacgggcc aagcttcact cacgatagta aggcttttgc gcagcgtacc ctgtgcggca
    11041 acggcagcat cacagctttg gttaccgaca tcagttcgat ctccaccggc tgggcgggca
    11101 tcgtgatgcg cgaaaacaaa gaccccaacg ccaaaaaggc gcagttgatg accaatctga
    11161 gcaatttgag ccgccgcgag ttccgcactg ctaccaacgg gcaggcatat ccgcagcagt
    11221 tcccaagcca aaaccgctac tggctgcgca ttacgcgtac gggcaatcag tttgtgatgt
    11281 atgtttctcc caacggaaca agctggtttg ttgtaggctc gcaaaacatc gtgatgccgt
    11341 cttgcatcct catgggcttg gtcgtcacca actatacgcc tgtcagcacc gtgacggcaa
    11401 gcttctctaa tgtgagcttt acgggcggca acagcggcct tggctcgccc ttcgcccctg
    11461 acgtcttcac ccctctccac tcacccctca cccctgattt cgatgtgttt cccaacccga
    11521 cgagcggcga cctgaatgtg aatctgtcgc agtacatcgg tcgcagtgtg cgcattgaaa
    11581 cgtacagcct cgaaggcaag ctgctgcaat ttactgaatt ggacgaagtg caacatacgc
    11641 cggaacgcct tgatatgaaa ggcttgcaaa ccggtatgta cttggtgaaa gtgaaa
//
LOCUS       DAORLF010000095        11615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2956_length_11615_cov_2.402682, whole genome shotgun sequence.
ACCESSION   DAORLF010000095 DAORLF010000000
VERSION     DAORLF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2956_length_11615_cov_2.402682"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>886
                     /locus_tag="PK198_09620"
     CDS             <1..>886
                     /locus_tag="PK198_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39036.1"
                     /translation="MNSKNPYAYQGLSVQVKSGITTSEAGGTNPYNLLSARYAKAFGK
                     FAFKLNASYLAATDWTANDYQTGRETANNRNPAGVGAPNFDGLNTYGDETEIVVPMVA
                     VAGSLSQALAPLFAPQLGIPVAQAQALLAQTIPRLPSLDLRRTGFTEADLLESNEAKS
                     VKLDGSLHYRISDNLEASYSYRYGSGSTVYQGGERYVLRNFSQQFHKIELSNPNFWLR
                     GYMSQTDDGDSYNLSALGAFANERFKPSSSAWVPTYAGTYAGALLPIYLQGGTANAQQ
                     LAGAHAAARNAANNGIPAV"
     assembly_gap    887..1007
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1008..2203
                     /locus_tag="PK198_09625"
     CDS             <1008..2203
                     /locus_tag="PK198_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39037.1"
                     /translation="NFREIISPSVVELLIGGNFRQYDLYSDGTIFNEDPDGDGKFERI
                     AINEFGGYLQASKRLFNDALKLTGSIRYDKNENFDGQFSPRVSAVYTAGANRQHNIRA
                     SYQTGFRNPSTQNQFIFFPTGQGNLVGSTEANDARYGIHNGGAYTNASYNAFIGSVLA
                     GQPNPTLLVERNLAYIQPEQLKAIEFGYKGLVTPKFLVDLSVYHTIYNDFITQETVRA
                     KTSSTHRGVTLPGVQQLLAGQASSATAWRPYINAEEQVTSTGVGLGLAYQLPKGYSLY
                     GNYTYSTFGVDNPSPGFEAGFNMPEHKFVVGLGNRKAFKNFGFDVNYRWQDEFDWENS
                     FAFGTIPAYGLLNLQASYALPKLKTVVKLGGSNAFGNDYRTNAGGPWVGQMYYVSLTF
                     DEFMK"
     gene            complement(2290..>2921)
                     /gene="radC"
                     /locus_tag="PK198_09630"
     CDS             complement(2290..>2921)
                     /gene="radC"
                     /locus_tag="PK198_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRF39038.1"
                     /translation="HLSDAELLALLLGSGTRDESAVGLAKRILHAAGNNLHELGKYSI
                     SDLIKKFKGVGEAKAITIMAALELGRRRQLSDIRERPKVNSSRDAYDAIAPLIGELPH
                     EEFWILLLNRSNQIIGREQISVGGTYGTIVDPRVVFHRALEARAAFVILCHNHPSGAL
                     YPSHADVALTNRLAKAGKILDIPVLDHIIVTEKGYYSFLDEGKMETVSV"
     assembly_gap    2922..2937
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3200..3451)
                     /locus_tag="PK198_09635"
     CDS             complement(3200..3451)
                     /locus_tag="PK198_09635"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRF39039.1"
                     /translation="MENKAKYTETLKTKTRTYFLDLKESSKGTAYLSVAESRKNKEGA
                     FETVRLSVFQEDIPEFAAAMARLVEQFGTQQQSAEPATA"
     gene            3560..3946
                     /gene="rnpA"
                     /locus_tag="PK198_09640"
     CDS             3560..3946
                     /gene="rnpA"
                     /locus_tag="PK198_09640"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRF39040.1"
                     /translation="MPDNQFKKNEKLKSRKRIAGLFEGGQSFAKYPLRVVWAAVEQRE
                     GEAAVQFGVSVAKKKFPKAVHRNRIRRLVREAWRLHKYRLMEAAPGKQQALAVMMLYT
                     GTQELPFAEIEAAMKELIHRLLKKWH"
     gene            4039..4857
                     /locus_tag="PK198_09645"
     CDS             4039..4857
                     /locus_tag="PK198_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HRF39041.1"
                     /translation="MKNGKLVTTGVVAFVILLVILMFSNATFLTIESGERGVLFKRFS
                     GGLDKANIYQPGFHVIAPWNSMYVYDVREQQLEEEMEVLSSNGLTIRVDITVRVNPRY
                     DKIGSLHEKFGKDYVTSLVRAEVRSSVRKIIGRFQPEELYSSKREEVQQLIYKDLENA
                     LRNNFIELRAALIRDIKLPDKVRLAIEDKIEAEQKALKYEYILEQERKEAERRIIEAG
                     AKAKANQILNASLTANILKDKGIEATLQLANSPNSKVIVVGSGQDGLPLILGNQ"
     gene            4963..>5350
                     /locus_tag="PK198_09650"
     CDS             4963..>5350
                     /locus_tag="PK198_09650"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF39042.1"
                     /translation="MEAILEIPVISAAETLLPGAEEQRVEGSYDDFTALLKQCDYPIE
                     YENGNIIIMSIASDEHEKIVANLLGALFIAMRGHRHLGRYGSNRHIYIPALSKAYSPD
                     ASVVKGNPDVFTYAVGKDAYTNPWLVA"
     assembly_gap    5351..5360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5361..5595
                     /locus_tag="PK198_09655"
     CDS             <5361..5595
                     /locus_tag="PK198_09655"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39043.1"
                     /translation="SPSSRSRDFGEKLHGYKAIPELQYILYIEQERPFATLFERIEGT
                     LRWRSADYDALNQSFFISEFSVAMSDLYENLKF"
     assembly_gap    5790..5903
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5904..6532)
                     /gene="thyA"
                     /locus_tag="PK198_09660"
     CDS             complement(<5904..6532)
                     /gene="thyA"
                     /locus_tag="PK198_09660"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRF39044.1"
                     /translation="MHVYHDLLRHILTHGDRKDDRTGTGTLSVFGYQMRFDLAAGFPL
                     LTTKKIHVKSVVHELLWFIAGDTNIRYLALNGVNIWNDWPFQHWLGATGQAADYPKYS
                     QAWREKMKDFVEQIKADEEFAARWGELGPVYGRQWRNFEGVDQLSEVIESIKKNPDSR
                     RLIVSAWNPKDIPVMVKSGLPPCHTLFQFYVSGGRLSCQLYQRSADVFLG"
     gene            complement(6818..7990)
                     /locus_tag="PK198_09665"
     CDS             complement(6818..7990)
                     /locus_tag="PK198_09665"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRF39045.1"
                     /translation="MKQSSAFQQNTRVGILGAGQLGKMLAIAAADWHLPIHALDRSRD
                     FPAAPYCAQFTEGDFKDPEAVYAFGKTVDVLTIEIEHVALDALFRLESEGVVVHPRPS
                     ALAVIKDKGLQKQFWVQHGLPSSPFRLYDSGEALREALRSGREKYPIVQKARTEGYDG
                     RGVAVIRSEKYLPKILDAPCLAEDLVPIHKELAVIACRNASGETKAFPAVEMTFHSEA
                     NLVEFLLCPARIDPHVEKAAEELALHAIEALDVCGLLAVELFLTTSGELLINEVAPRP
                     HNSGHHTINNSYTSQFEQHLRGILNLPLGSVQVQSPAAMLNLLGEPGHSGPAHYQGME
                     ACLAMEGVKVHLYGKAMTQPHRKMGHVTVLAESLEAAIEKAEQVRDVLKVVAGQER"
     gene            8218..8694
                     /locus_tag="PK198_09670"
     CDS             8218..8694
                     /locus_tag="PK198_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF39046.1"
                     /translation="MSQLLIAMTNHTAEIDWKADTSGFVLERTARRMKQFVQEHLTQA
                     GAGITVDQWLILQELDRQDGQSQLDIAKATYKDPPTMTRILDILVEKGLVHRAHDPAD
                     RRRFNILLTPAGRSKIEEVLPLVRAARREAWGGLSEPEMAELTRMLDAVFQHLQGQ"
     gene            8940..10091
                     /locus_tag="PK198_09675"
     CDS             8940..10091
                     /locus_tag="PK198_09675"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRF39047.1"
                     /translation="MHYHSLGQIPHKRHIQFRQPDGSLYHEELFSTEGFSNAYSILYH
                     CNAPTQIVQTGEPYSVAPKTVHDKQLKHRSLKGFRIEPEDDYLAARKPVLVNDDCRII
                     LAAPRQSMTGYFFKNADADEMIFIHKGTGKLLTMYGELPFEYGDYVVVPRGTTYQLHF
                     DTPDNRLFIVESFGPIVPPKRYRNAIGQFEEHAPFCERDIRKPQNLATHNEQGEFLFY
                     IKKQDVIYPYYYLNHPFDVVGWDGYVYPYAFSIHQFEPITGRVHLPPPIHQTFDGRNF
                     VICSFVPRLYDYHPQAIPAPYNHSNIDSDEVLYYVDGDFMSRKHVEPGMITLHPGGIP
                     HGPHPGTVEKSIGAKETRELAVMVDTFRPLLMTEYAAQIEDGDYFKSWL"
     assembly_gap    10113..10129
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10182..11306
                     /gene="hppD"
                     /locus_tag="PK198_09680"
     CDS             10182..11306
                     /gene="hppD"
                     /locus_tag="PK198_09680"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRF39048.1"
                     /translation="MSTLTETITDLAQDTFPIKGTDHVEFYVGNAKQAAHYYQSAFGF
                     ELVAYAGPETGLRDRASYVLQQGKIRFVLSSALGPESEIAQHVHLHGDGVKVLALWVD
                     DAEQAYYTALERGAVSAAAPRTISDEHGSVTLASIHTYGDTWHTFVERNHYNGAFMPG
                     FQPRSSEFQSKPIGLKYVDHCVGNVELGQMNRWVQFYQDVMGFNLLVTFDDKDISTEY
                     TALMSKVVSNGNGFIKFPINEPAQGKKKSQIEEYLDFYRGAGVQHIAVATDDILHTVG
                     ELRSRGVEFLYVPDTYYETVTERVGTINEDIEDLKRLNILVDRDEEGYLLQIFTKPVQ
                     DRPTVFYEIIQREGARSFGKGNFKALFESIEREQALRGTL"
     gene            complement(11310..>11615)
                     /locus_tag="PK198_09685"
     CDS             complement(11310..>11615)
                     /locus_tag="PK198_09685"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HRF39049.1"
                     /translation="TRNARHGMLFTAEGIINIRNNKWKDDYSPIDENSPSTTSRYHTK
                     AYLRHLIYNHEILGAQIASLHNLSFFLWLVGEARAHIQAGDFAHWKTGMVERLKRRL"
BASE COUNT         2700 a         3216 c         2910 g         2511 t
ORIGIN      
        1 atgaacagca aaaacccgta tgcctaccag ggattgagcg tgcaggtcaa gtcgggtatc
       61 actacttccg aagcaggcgg caccaatccg tacaacctcc tttcggcgcg ttatgccaag
      121 gctttcggaa aatttgcctt caaactcaac gcatcctacc tcgctgctac cgactggacg
      181 gccaacgact accaaaccgg ccgcgaaaca gccaataacc gcaacccggc aggcgtaggc
      241 gctcccaatt ttgacggcct caatacctac ggcgatgaaa ccgaaattgt ggtgccgatg
      301 gtagccgtag caggcagcct ttctcaggct ttagcgccct tgtttgcgcc tcaattaggc
      361 atccctgtgg cacaggcaca ggcgctgctg gcacaaacca ttccaagatt accttctctc
      421 gaccttcgcc gtactggctt taccgaagcc gaccttttgg aaagcaacga ggccaagagt
      481 gttaaattag acggctcact ccactaccgc atctccgaca acctcgaagc atcttacagc
      541 taccgctacg gctcaggttc tacggtgtat cagggtggcg agcgctatgt gctgcgcaat
      601 ttctcgcagc agtttcacaa aattgagttg agcaatccca atttctggct gcgcggctac
      661 atgtcgcaaa ccgatgacgg cgactcttac aacctttctg ctctgggcgc atttgccaat
      721 gagcgcttca agccgtcctc ctcggcatgg gtacccactt acgcaggcac ttatgcagga
      781 gcattgttgc ccatttacct gcaaggcggc acggccaatg cccagcaatt ggcaggcgct
      841 catgctgctg ccagaaatgc ggccaacaac ggcattcctg ccgtcgnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca atttccgcga
     1021 aatcatctcg ccgagtgtgg tagaactgct catcggcggc aacttccgtc agtatgacct
     1081 ctactccgac ggcaccatct tcaacgaaga cccggacggc gacggcaaat tcgaacgcat
     1141 tgccatcaac gagtttggcg gttatttgca ggcttccaag cgcttgttca acgacgcgct
     1201 caagctcacc ggctccattc gttatgacaa aaacgaaaac ttcgacggcc agttttctcc
     1261 acgcgtttct gcggtttata ccgccggcgc caaccgccag cacaacatcc gcgcttcgta
     1321 ccagaccggc ttccgcaatc cgagtacgca aaaccagttc attttcttcc ccaccggcca
     1381 gggcaatctc gtcggcagca ccgaagccaa tgacgcccgc tacggcatcc acaacggcgg
     1441 ggcttatacc aatgcttcct acaatgcctt catcggcagc gtattggctg gccaacccaa
     1501 tccgactttg ctggtagaac gcaatctggc ttatattcag ccggaacaat tgaaagccat
     1561 cgaatttggc tataaaggct tggtaacgcc caaattctta gtggacctga gcgtctatca
     1621 taccatttac aacgacttca tcacgcagga aaccgtgcgc gccaagactt ccagcacgca
     1681 tcgcggtgta acactgccgg gcgttcagca attgttggcc ggtcaggcat cgtctgctac
     1741 ggcatggcgc ccgtacatca atgcggagga gcaggtgacc tctaccggcg tgggcttggg
     1801 cctggcatac cagcttccga aaggctactc gctgtacggc aactacacct actccacgtt
     1861 tggcgtagat aacccatcgc cgggcttcga ggccggtttc aacatgccgg agcacaagtt
     1921 tgtagtgggc ttgggcaacc gcaaagcatt caaaaatttc ggcttcgacg tcaactaccg
     1981 ctggcaggat gagttcgact gggaaaacag ctttgcattt ggtaccattc cggcatacgg
     2041 cctgctgaat ctgcaagcca gctatgcgtt gcccaaactc aaaactgtag taaaattggg
     2101 aggttccaac gcattcggca acgactaccg caccaatgca ggcggcccct gggtagggca
     2161 gatgtactac gtttccttaa cgtttgatga atttatgaag taaaaatgga ggcaggaaaa
     2221 gattttttcc tgtcctgttg attacaaaga ccgtttcggc cgtcgtgtcg gagcggtctt
     2281 tgtcgttttt tatacactca ctgtttccat cttaccctca tccaaaaagc tgtaatagcc
     2341 tttctcggtc acgatgatgt gatcaagcac aggtatgtca aggattttgc ccgctttggc
     2401 caagcggttg gtgagggcca cgtcggcatg gctgggatac aaggcgccgg agggatgatt
     2461 gtggcaaaga atgacaaaag ccgcacgcgc ctccaaagcc ctgtggaaca ccacacgcgg
     2521 gtcaacaata gtgccataag tgccgccgac gctgatctgt tcgcgcccga tgatctggtt
     2581 ggagcggttg agcagtaata tccaaaactc ttcgtggggc aactcgccga tgagcggcgc
     2641 aatggcatca taggcatcgc ggctgctgtt caccttgggt cgttcccgga tatcgctgag
     2701 ttggcgtcgg cggcccagtt ccaatgctgc cataatggta atggctttgg cttcgcctac
     2761 gcctttgaac tttttgatga gatcgctgat ggagtacttt cccagttcgt ggaggttgtt
     2821 tccggcagcg tgcaaaatgc gcttggccaa tccgacggcg ctctcgtcgc gggtgccgga
     2881 gccaagcaaa agcgccagca gttcggcgtc gctgaggtgg cnnnnnnnnn nnnnnnntgc
     2941 ccctgagcag cattttttcg cggggccggt cttcttcggc ccaggcacga atagggaggg
     3001 gcggaaccgt gtaagacgac ggcttctgtt ctttatcggc agaagcagtt ggggcggcta
     3061 aatcgggcga agtggacgtc attgggtagc ttgctgaatg gagtgttgaa aaaaatggat
     3121 ggcgccggga agccggtagt tgttcgtggg atcagacaac tgttatacag accgattcca
     3181 ggcgccattg tcacgggggt caagcagtgg caggctctgc cgattgttgc tgcgtgccga
     3241 attgctcgac gaggcgggcc atagcagcgg caaactcagg aatatcttcc tgaaaaacag
     3301 aaagacgaac agtttcgaag gctccttctt tatttttgcg gctttcggcg acggagaggt
     3361 aggccgtgcc tttggagctt tctttgaggt ctaagaaata cgtacgggtt tttgttttga
     3421 gagtttcggt gtactttgcc ttgttttcca tggcgattgt tacttttaga gtttaaaaaa
     3481 aattgctgtc actcatatag tcgttgggtc ggcaaaaatt ccacaacaga attttaaaaa
     3541 aatgaaaata attttttaaa tgcctgataa tcaatttaaa aaaaatgaaa aattaaaaag
     3601 tcgcaagcgc attgccggtc tgtttgaggg tgggcagtca tttgcaaagt accctttgcg
     3661 cgtggtttgg gcggcagtag agcagcggga aggcgaggct gccgtgcagt ttggagtaag
     3721 cgtagcgaag aagaaattcc ccaaagccgt acaccgcaac cgcatacgcc gcttggtgcg
     3781 cgaagcatgg cgattgcaca aatacaggct catggaagca gcccccggca agcagcaggc
     3841 attggcggtg atgatgttat ataccggcac acaggagttg ccttttgccg aaatagaagc
     3901 agccatgaaa gaattgattc accggctatt gaaaaaatgg cattaatgac ccttctgtgc
     3961 ggtcttatcg aaattttcag cctctcggga gcaaaaaaat attacttttg ggaagtataa
     4021 accaaaatac ttatcacaat gaaaaacgga aaacttgtaa ctaccggtgt ggtcgcgttt
     4081 gtcatcttat tggtgattct gatgttttcc aacgccactt tcctcaccat tgagtccggc
     4141 gaacggggcg tattgttcaa acgcttcagc ggtggcttgg acaaagccaa tatctatcag
     4201 cctggttttc atgtcattgc cccctggaac agcatgtatg tgtatgacgt gcgcgagcag
     4261 caattggagg aagaaatgga ggtcttgtcc agcaatggcc tcaccatacg ggtggacata
     4321 actgtgcgcg tcaatccgcg ctacgacaaa atcgggtcgc tgcacgaaaa attcggcaaa
     4381 gattacgtca cctctttagt gcgggccgaa gtgcggtctt ccgtgcgcaa gatcattggc
     4441 cggttccagc ccgaagagtt gtattcgtcc aaacgggaag aggtacaaca actgatctac
     4501 aaggatttgg agaatgcgct tagaaataac tttatagaac ttcgcgccgc cctcattcgc
     4561 gatatcaaac tgccggataa agtgcgctta gccattgagg acaaaattga ggccgagcaa
     4621 aaagccctca agtacgagta catcctcgaa caggagcgca aagaggccga gcgcagaatc
     4681 attgaggcag gcgccaaagc aaaggccaac cagattttga acgccagcct tacggccaat
     4741 atcctcaaag acaagggcat agaggctacg ctgcaattgg ccaactcgcc caattccaaa
     4801 gtcattgtgg tgggaagtgg tcaggatggt ttgccgctga ttttgggcaa ccagtaactc
     4861 ccgcttttct gaagagcaaa caagggtcgg cagcgcggtt gccggccctt gttttttttg
     4921 ccccccttgc gattccttca aaaaccgctt atctttgccc ttatggaagc gatactcgaa
     4981 atacccgtta tttccgcagc cgaaacgctg ctgcccggcg ccgaagaaca gcgcgtggaa
     5041 ggtagctacg acgattttac ggctctgcta aagcaatgcg actaccccat cgagtacgaa
     5101 aacggaaaca tcatcattat gagcatcgca tcagatgaac acgagaaaat cgtagccaat
     5161 ttattaggag cgctttttat tgccatgcgc ggccatcgcc atttggggcg ctacggcagc
     5221 aatcgccaca tctacatccc ggcgctcagc aaggcatatt ctcccgatgc ttccgtcgta
     5281 aaaggcaacc ccgatgtgtt tacttatgct gtcgggaagg atgcttatac caatccctgg
     5341 ttggtggccg nnnnnnnnnn ttcgccctcc agccgcagcc gcgattttgg cgaaaaactg
     5401 cacggataca aagctattcc cgaactgcaa tacatcctct acatagagca ggagcggcct
     5461 ttcgcaacac tttttgaacg catagaaggc acgctccgct ggcgcagcgc cgactatgac
     5521 gcgctcaacc agtcgttttt catcagcgag ttttcggtgg ccatgagcga cttgtacgag
     5581 aatttgaaat tctgaccctg ttctttcaaa acggccccat cacacagcca cctccgcctt
     5641 gatatgcgag tgcgggtcgt aatctctaag gtggaaatgt tcgtaccgaa attccaaaat
     5701 atcgttgatg cccgactcta tttccatgcg cggcaagggg cggggttccc ggctgagttg
     5761 caattgcgcc tgttcgatgt ggttgctgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnncccagaa atacatcggc cgaacgctgg tacaactggc
     5941 acgacaaacg cccgcccgac acataaaact ggaacagcgt atggcagggc ggcagacccg
     6001 atttcaccat caccggaatg tctttggggt tccaggctga aacgataagc cgccgcgagt
     6061 cggggttctt tttaatactt tcgatgacct ccgacaattg gtccactccc tcaaagttgc
     6121 gccactgccg gccatacacc gggccgagtt cgccccagcg ggcagcaaat tcttcgtcgg
     6181 ctttgatctg ctccacaaaa tccttcatct tttcgcgcca ggcctggctg tatttaggat
     6241 agtcggcggc ctgtccggta gctcctaacc agtgttgaaa aggccagtca ttccagatgt
     6301 tgacgccgtt cagcgccaga tatcgaatat tggtatcgcc ggcaatgaac caaagcagct
     6361 cgtgaaccac cgatttgacg tgtatttttt tggtggtcag caaagggaaa cctgctgcga
     6421 gatcgaatcg catctgataa ccgaaaacgc tgagcgtgcc ggtacccgtg cggtcgtctt
     6481 tgcggtcgcc gtgggtgaga atgtgccgga gcaggtcgtg gtatacgtgc atgaatggtc
     6541 tgtgtttggg tgataaaggt ataaaatttc aaaggatagg cgggatgcta cgctccgaca
     6601 atcgcattaa aaaggcaaaa tgcaaaaccg taaaatgcaa aaccgcaaag tgcgtagcgt
     6661 actctccgcg agggggcagt ctcgtttact tttggggtac tcctacggtt gatttttaca
     6721 ttttacattt gatattttgc ggttttgcgg tttcccgccc gcgcaaggtt ctaccttgtg
     6781 cggactattc ggcgggttct acccgccatg gtctgcctta tcgctcttgc cccgccacca
     6841 ctttcagcac gtcccgcacc tgctccgcct tttcgatggc cgcttcaagg ctttccgcca
     6901 aaacagtaac atgccccatt ttccgatgag gctgtgtcat ggctttgccg tacagatgca
     6961 ccttgacgcc ctccattgcc aaacaagcct ccatgccctg ataatgcgcc ggaccggaat
     7021 gccccggttc gcccagtaaa ttgagcatcg ccgctggaga ctgcacttgc acgctgccca
     7081 atggcaggtt gaggataccg cgcaggtgct gctcgaattg tgaggtataa ctattgttga
     7141 tggtgtgatg cccgctgttg tgcggtcgcg gcgccacttc gttgatgagc agttcgcccg
     7201 acgttgtcag aaacaattcc accgccagca aaccgcacac atccagcgcc tcaatggcat
     7261 gcagggccaa ttcctcggcg gctttttcta catgcgggtc aatgcgggcc gggcagagca
     7321 gaaactccac cagattcgct tccgaatgaa aggtcatttc caccgccgga aaggctttcg
     7381 tctcgcccga agcattgcgg caggcaatga cagccaactc tttgtgaatg ggcacgaggt
     7441 cttcggcgag gcagggcgca tccagaattt tcggcaaata tttttccgag cgaatcaccg
     7501 ccacgccgcg tccgtcgtag ccctcggtgc gggccttttg tacgatcgga tacttttccc
     7561 gccccgaccg gagtgcctcg cgcaaagcct ctccgctgtc gtacaggcgg aaaggggaac
     7621 tgggcaggcc atgttgcacc caaaactgct tttgcaaacc cttgtctttg atgactgcca
     7681 gcgctgacgg gcgaggatgc accaccacac cctccgattc gagccgaaac aaggcatcca
     7741 gggccacatg ctctatttcg atggtgagca cgtccaccgt tttgccaaac gcatacaccg
     7801 cttcggggtc tttaaaatcg ccctccgtaa actgcgcaca gtagggggcg gcaggaaaat
     7861 cgcgggagcg gtcgagcgca tgaatcggca aatgccagtc ggcggcggct atggccagca
     7921 tcttgcccaa ttggccggct ccaagtatgc cgacacgcgt attttgctga aaagcagagg
     7981 attgtttcat cgtaatgatt tctttcggca gtttgtttgc ctcattgaag gcgcaaagat
     8041 aaagtgccgg gccggtaaaa agtaaggtaa caattcagtc gcctgctgaa agctgcgagc
     8101 cgcgagtttc gagccgcgag gcagactcat aagctcgtga ctttggggaa ccggctatat
     8161 aggcacgaca gaaaaaatgc aaaaataatt tgcacataat tagcacaaca actatttttg
     8221 tcccaattat tgattgccat gaccaaccac accgccgaaa tagactggaa agcagatacc
     8281 tccggcttcg tgctggaacg caccgcccgg cgcatgaaac aattcgtgca ggaacacctc
     8341 acacaggccg gcgccggcat caccgtggac cagtggctca tcctacagga attggaccgg
     8401 caagacgggc aaagtcaatt ggacattgca aaagctacct acaaagaccc gcccaccatg
     8461 acgcgcatcc tcgatatttt ggtggagaaa ggtctggtcc atcgtgctca cgatcccgcc
     8521 gaccgacgca ggttcaatat cctgctcacg cccgccggcc gtagtaaaat tgaagaggta
     8581 ctgccgctcg ttcgcgccgc ccgccgggaa gcctggggcg gcctcagcga acctgaaatg
     8641 gccgaactca cgcgcatgct cgacgctgtt tttcaacacc tgcaagggca gtagcaaagc
     8701 gtttcgggtt ccgcatttcg ggttcaaggt tccgggttgc atctgccgga gccttggcga
     8761 aggtgggttc aatgttcctg gttgccattc tacgttcgcg gaatttcgcg cccttcgcta
     8821 aaattcgcgt taaaaatttt ggcgttccgg caggaacggg cgggtttggg gttcaaggtt
     8881 ccgcgttccc acacgaaatc agaaatcaga aatcagaaat cagaaatcag aaatcagata
     8941 tgcactacca cagccttggc caaataccac acaagcggca catccagttc cgccaacccg
     9001 acggcagtct ctaccacgaa gagctcttct ctaccgaggg cttcagcaat gcctactcca
     9061 ttttgtacca ttgcaacgcg cccacccaga tcgtgcaaac cggcgaaccc tactccgtag
     9121 cgcccaaaac cgtacacgac aaacaactca aacaccgcag tctcaagggc ttccgcatag
     9181 agccggaaga cgactacctc gccgcccgca agcccgtact cgtcaacgac gactgccgca
     9241 tcatcctcgc cgcgccgcgc caaagcatga cgggctactt cttcaaaaat gccgatgccg
     9301 acgaaatgat cttcatccac aaaggcactg gcaagctcct caccatgtac ggcgaactgc
     9361 ccttcgagta cggcgactac gtggtcgttc cgcgcggcac cacctaccaa ctgcattttg
     9421 acacacccga caaccgtctc ttcatcgtcg aatcgtttgg ccccatcgtg ccgcccaaac
     9481 gctaccgcaa cgccatcggg cagttcgagg agcacgcccc gttctgcgag cgcgacatcc
     9541 gcaaaccgca aaacctcgcc acgcacaacg agcagggcga attcctgttt tacatcaaaa
     9601 aacaggacgt catctaccct tattactacc tcaaccaccc tttcgatgtc gtcggctggg
     9661 acggctacgt ctatccctac gccttctcca tccatcagtt cgagcctatc accggccgcg
     9721 tacacctgcc gccgcccatt caccaaacct ttgacgggcg caattttgtg atctgttcct
     9781 tcgtcccgcg cctctacgat taccacccgc aggccattcc cgcgccctac aatcacagca
     9841 acatagacag cgatgaagtg ctgtactatg tggacggcga cttcatgagc cgcaaacacg
     9901 tcgagccggg tatgatcacc ctccaccccg gaggcatccc gcacggcccg catcccggta
     9961 cggtagaaaa gagcatcggc gccaaagaaa cccgcgaact cgccgtcatg gtggacacct
    10021 tccgccccct cctgatgacc gagtacgccg cacaaatcga agacggagat tatttcaaaa
    10081 gctggttgta ggagctgtta gctttagcat gannnnnnnn nnnnnnnnnc aagctaacag
    10141 ctctctgcct gcagatacca aaataaatat caaaaaaaac catgtcaacc ttaaccgaaa
    10201 ccatcaccga cctcgctcag gataccttcc cgatcaaagg taccgaccac gtcgaatttt
    10261 atgtaggcaa tgccaaacaa gccgcccact actaccagtc ggccttcggg tttgaactcg
    10321 tagcctacgc cggccccgaa accggcctcc gcgaccgcgc ctcttatgta ctgcaacagg
    10381 gcaaaatccg tttcgtcctc agctcggccc tcggcccgga aagcgaaatc gcacagcacg
    10441 ttcacctgca cggcgacggc gtcaaagtgc tggccctctg ggtggacgat gccgaacagg
    10501 cttattacac cgccctcgaa cgcggcgccg tttcagccgc agcaccccga accatctccg
    10561 acgaacacgg ctccgtcacc ctcgcctcca tccataccta cggcgatacc tggcacacct
    10621 tcgtggagcg caatcactac aatggcgcct tcatgccggg tttccagccc cgtagcagcg
    10681 agttccagtc caagcccatc ggcctgaaat atgtggacca ttgtgtgggc aatgtggaac
    10741 tgggccagat gaatcgctgg gtgcagtttt atcaggacgt catgggcttc aacctgctcg
    10801 tcaccttcga cgacaaagac atctccaccg aatacaccgc cctgatgagc aaggtggtga
    10861 gcaatggcaa cggcttcatc aaattcccca tcaacgagcc ggcgcagggc aagaaaaaaa
    10921 gccagattga ggaatatttg gacttctatc gaggggcggg cgtacagcac atcgctgtag
    10981 ctaccgacga tattttgcac acggtaggcg aattgcgcag ccgcggcgtg gagttcctct
    11041 atgtgcccga cacttactac gaaaccgtga cagagcgcgt gggcacgatc aacgaagaca
    11101 tcgaagacct caagcgcctc aacatcttag tggaccgcga cgaggagggc tacctgctcc
    11161 agattttcac caaaccggta caggatcgcc ccacggtgtt ctacgaaatc atccaacgcg
    11221 aaggcgcacg cagtttcggc aaaggcaatt tcaaggcttt gtttgaatcc atcgaacgcg
    11281 aacaggcgct gcgggggacg ttgtaatcct cacaacctcc gcttcagccg ctccaccatg
    11341 cccgttttcc aatgggcaaa atcgccggcc tggatgtgcg cgcgggcctc gcccaccaac
    11401 cacaggaaga agctgaggtt gtgcagcgag gctatttgtg cgcccaaaat ttcgtggttg
    11461 taaatcaggt ggcgcaggta tgccttggtg tggtagcgac tggtagtgct ggggctgttt
    11521 tcgtctatcg ggctataatc gtctttccat ttgttgtttc tgatgttgat gatgccctct
    11581 gcggtgaaga gcatcccgtg acgcgcattg cgggt
//
LOCUS       DAORLF010000096        11546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_2985_length_11546_cov_2.004525, whole genome shotgun sequence.
ACCESSION   DAORLF010000096 DAORLF010000000
VERSION     DAORLF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_11546_cov_2.004525"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK198_09690"
     CDS             <1..206
                     /locus_tag="PK198_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HRF39050.1"
                     /translation="STGLATGPHVCFRFWKNGQQVDHLRLDLPQAKPLPESEMPLFKP
                     VKDAFLEKLKAEKPVEAVKKSNP"
     assembly_gap    269..327
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1879..3381
                     /locus_tag="PK198_09695"
                     /pseudo
     CDS             1879..3381
                     /locus_tag="PK198_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
     gene            complement(3687..4385)
                     /gene="folE"
                     /locus_tag="PK198_09700"
     CDS             complement(3687..4385)
                     /gene="folE"
                     /locus_tag="PK198_09700"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRF39051.1"
                     /translation="MTKTTFNGNGHHAASKGKLNGHSNYYLLEEDPDFDTPMRPDAFL
                     KSEEEKMNIIEDHFKVIMETLGLDLSDDSLRGTPRRVAKMFVQEIFSGLDPENKPNIS
                     LFENKYRYNQMLVERNITVKSFCEHHFLPITGVAHVAYFSSGQVIGLSKLNRIVEYFS
                     RRPQVQERLTIQIAEELKKALNTEDVAVYLDARHMCVELRGAEHENCSTVTTEYSGKF
                     LQESVRAEFLQSIR"
     gene            4547..5056
                     /locus_tag="PK198_09705"
     CDS             4547..5056
                     /locus_tag="PK198_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39052.1"
                     /translation="MKPTQLLLFALLLVAFSQCSGGPKKLEDCPYGKPKAIFSEQMPG
                     VTRHSFRTDDLSATEELTLGDSLSVTLIQSGCEKPVQEFRFVIQNPPPQADAAYWADR
                     GVDLLYQLSSLSPELVPLSAWAQAVEQQIPGISLGEPFPVEQDTYVSIDRIESAGNVI
                     LMLILGAEP"
     assembly_gap    5083..5095
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5501..>5908
                     /locus_tag="PK198_09710"
     CDS             5501..>5908
                     /locus_tag="PK198_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF39053.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVAVVERESLGGICLNWG
                     CIPTKALLKSAQVFEYIQHASDYGIQVSGAQADFGAMVKRSRGVADGMSKGINFLFKK
                     NKITVINGNGRLVRGKKVEVTAAAGAKTEYSAEH"
     assembly_gap    5909..6150
                     /estimated_length=242
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6151..6910
                     /locus_tag="PK198_09715"
     CDS             <6151..6910
                     /locus_tag="PK198_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF39054.1"
                     /translation="DVSKELAKIYQKAGIEVLANTSVETVDTKGAGCKVTVKSRKDGS
                     VKQIDCDVVLSAAGVTPNTESIGLEELGVKTDRGFVLVDDYYRTNVPGIYAIGDIIPT
                     QALAHVASAEGITCVEKIAGHTPDPIDYNNIPSCTYCSPEVASVGLTEQAAKDAGYTL
                     KVGKFPFSASGKASAAGAKQGFVKVIFDAKYGEFLGAHFIGANVTEMIAEVVVARKLE
                     TTGHEIIKAIHPHPTMSEAVMEAAAAAYDEVIHL"
     gene            complement(6992..>7611)
                     /locus_tag="PK198_09720"
     CDS             complement(6992..>7611)
                     /locus_tag="PK198_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRF39055.1"
                     /translation="VAHNGGSISLRRFLQQNNMQQEYCGLVNISNMQNVYALYYGEDS
                     GYKGIFQKSNSDDISLSSIPKGAPQVAWMYFNKNGFSVYCREYREYWEWVENRNEARY
                     QSTLHHGKNYDAKNMMHTFRLLEMAIEIARHKKIVVRRPDADFLLSIKKGAYDYEQLI
                     EMAAAKYDEMETAYAASDLPDAPDSGYLNDLAYEIRAEFYGIKSD"
     assembly_gap    7612..7624
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7625..8058)
                     /locus_tag="PK198_09725"
     CDS             complement(<7625..8058)
                     /locus_tag="PK198_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF39056.1"
                     /translation="MELQYLRNNRLLILEALSGSKAYGLDTPESDMDFRGVFMLPRRQ
                     FYGLQYIPQINLDNNNHMLYEWRRFVELLSVNNPNILELLETPADALIYKHPLLHQVD
                     CSKIVSRQCKETFGRFALSQVKKARGLNKKIVNPMDRERKTVL"
     gene            complement(8040..>8492)
                     /locus_tag="PK198_09730"
     CDS             complement(8040..>8492)
                     /locus_tag="PK198_09730"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF39057.1"
                     /translation="LEQIRSVSQDFYSRIGAMHHYLNMARNTLDEIDESRPYKLKKLF
                     YALRSATACRWILEKNEPPPVSFNDMLSGLDLPETILSDIERLLDIKSKADESYLHRG
                     ESNLLDFVRHSLFTATENGAQLPSGKGRSDVCNELFINTLERIWNSNI"
     assembly_gap    8493..8502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8503..8824)
                     /locus_tag="PK198_09735"
     CDS             complement(<8503..8824)
                     /locus_tag="PK198_09735"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF39058.1"
                     /translation="MVSKINAYIKQVESERGVKILLASETGSRAWGFPSPDSDYDVRL
                     IYAHPVQWYLSLSEKQDSMDTMLENRELDVSGWDLRKSLRLLLRSNPPLLERIHSPVV
                     YACDE"
     gene            complement(8880..9602)
                     /locus_tag="PK198_09740"
     CDS             complement(8880..9602)
                     /locus_tag="PK198_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRF39059.1"
                     /translation="MKAMIFAAGLGTRLRPLTNDRPKALVEVNGVPLLQIAIEKLHLA
                     GITDIIVNIHHFGEQIAAFLARPPFSDMNIAISDERAKLLDTGGGLRKAAWFFDDGQP
                     FLVYNADILTTLNLNDLYAAHTRSQALATLAVRERPASRAFLFDKNNRLCGWRNSATG
                     AERIALANHPLHPLAFSGVHIIHPRIFELMPPPGEVFSIVETYLSLAQNEIIQGFRHD
                     QDLWLDVGKPEQLARAQELWTW"
     gene            9770..10921
                     /gene="ppk2"
                     /locus_tag="PK198_09745"
     CDS             9770..10921
                     /gene="ppk2"
                     /locus_tag="PK198_09745"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRF39060.1"
                     /translation="MAKRRPTINEEEALKIVTREVSEGDVTGGETPSEARVKSVNRRI
                     RAKINHEATALKDAMESKPDDKKSAAEQYLQSFPVEERVLLLKTLLEENLQGQGKKVH
                     PDHELARDWREGGYPYKNLLSRKAYEAQKYDLQVELLKLQAWTKKTGARVVVLFEGRD
                     AAGKGGTIKRVMEHLNPRGARVVALEKPTEEEKGQWYFQRYVRHLPTFGEIVLFDRSW
                     YNRAGVENVMGFCTKEEYTEFMRQVPDFERNLVRSGIILIKFWFSVSREEQKRRFKDR
                     ETHPLKQWKLSPIDRASLDKWEDYTDAKEKMFFHTDTSDSPWIVVKSNCKKRARLNAM
                     RYVLHRIAYDNRDLKMIGKLDPLIVGRSNVIYEKGENLFLNDTLANMLK"
     gene            complement(10931..>11546)
                     /locus_tag="PK198_09750"
     CDS             complement(10931..>11546)
                     /locus_tag="PK198_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF39061.1"
                     /translation="YGLKCKEEFAVQKIPTVLIFNAKGEVIDRREASIDAGALLTLLQ
                     RYSREEAPRTQSAPAQPESPAYPMSGKTNSAPLAPGQFSRPALIPDMPHSQDVVQEMI
                     EPSVLQAASYSSSPVADAQTHYGPATTYTIQAAVFGDYGNAVNEVNRLEGLLRRPVSL
                     AASVQNGKTLYKITIGNFSSRQTADEYLRYLQSKSINGFVRASE"
BASE COUNT         2712 a         2985 c         2898 g         2614 t
ORIGIN      
        1 gttctaccgg tttggctaca ggcccgcatg tttgtttccg cttctggaaa aacggccaac
       61 aggtggacca cctccgtttg gatctcccgc aggccaagcc gctgcccgaa agcgaaatgc
      121 ctttgttcaa gccggtgaaa gacgctttct tggagaaatt gaaggcggaa aagccggtgg
      181 aggccgtgaa gaagtccaat ccgtaactgc gctatctgag gagcctgcga aacatccgcc
      241 ccgaagaagt caaatcaaaa ttacaatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa aaaggcatcg aaaaaggcat cgaaaaaggc
      361 atcgaaaaag gcatcgaaaa aggtgcgcga gaggtcattg tctctctcat tcatcatttg
      421 cccgatgcta atgacgaaac catcgccaat ttgtcttcca aaccggtgga gtatgtggca
      481 caggtgcgcc gggaaatgaa gtagaaattt cccttcaatt ataaacaagc agacctacgg
      541 ctataataca ctgaaaatcc tcgtaccttt ccccacgaaa tgaagaggat attcaaatat
      601 tccagaaatc ggacggtcgt ctcaaaaaag actatccctg catggcccac agcactgttg
      661 ctattgctgc ccttgacggg cgctttcgcg caacgctcag attatcaggt caaagtattg
      721 gggtttgaag aaggcctcat ccaccgcaac gtcttcaaag tgcaacaaga ccggcgcggc
      781 tacctctggc tcggcgccat caacgggctg gatcgctacg acggataccg cttcattcac
      841 tacaacagaa ccgaccagca gcacaccttg ccgacggagt atgtttccga tttgcacata
      901 gacgttcaca accatatatg ggtagctcac ccccggcaga tgtcgctgct gttgccggaa
      961 actaaccgga tcatatcggt gccgctgcca gatggaacgg cgccttccaa cctcaccgag
     1021 gatgccaaag gacgcgtatg ggcggctttg tttgacgaaa aaagcggcgc cacccgtttg
     1081 cagcgtttgg atactttaaa cccaaaaaca ctttccattc ccgtacccgg attgtatgcg
     1141 cgccgtccga tggccgcatt gggcaaacat ctgtacgccg gcgcctggga aaacgaactt
     1201 tggaaaatag acgccgctgg taaagtggta caaaaaatgc gcctgcccgc cgccggtcgc
     1261 caactttcca gaatcgtgca tttgcaggca tcgggcgaca gcctgttcat cctttgctcc
     1321 gacagtcggg ttttcacctg gcagcccgct tcgggcatgt tttcgctgca tcccgtcagt
     1381 tcgtttattc aggacaaagg caaagcagcc accctgctgg ccgaaccggg cggcaatgtc
     1441 tgggtggccg gccaaaacgt cttctggcat tacaatgccg ccgccgggca ggttacggat
     1501 atggacgaac cgattcgtca gtcggtcaaa aactacctga attacaggca aatactgatg
     1561 gatagaagcg gcgttgtgtg ggtcacttcc gattttggac tcattaaact tacccgctcg
     1621 gtgcgcctat ttaccaatta cctcagcggc ggcagcgagt attgcagcga cgggttttgc
     1681 agcacgcggg gcatcgccga agacgaccgg ggcaatattt acatctccta ttacaactcc
     1741 attcacgttc tcaatccgcg tacggaatac ctgcggccgc tgtttcccaa caacgatttt
     1801 cacaacattc cctacggcct gctttggaaa gacaatgcac tgtggacggg caacggcatc
     1861 cgcatagacc tcaaaaccat gcgcaccgat acgatttttg cgcagggtga ggccaactta
     1921 gcctccgtgt gcgccgatga ggacggcaaa ctttggttcg ggtaccgtaa gcacttgtac
     1981 tggtacgacc ccgaaacgcg caaaaccacg gaactgcaag acctgctcga acccaatttt
     2041 cagggcgaga tcagctatgt gtatcccggc aggaatggcc ggtatctttg ggtaggaacc
     2101 ctgcaacacg gggtctatcg cttagatgtg cagaatggcc gatcagatcg ctggcacctc
     2161 aacagcacac cggcgagttt ggcctccaac gaggtgaatg ccatctacga agatagccgg
     2221 ggctacgtct ggctctctac catcaacggg ctgcaccgac tgaaacccgc caccaacgaa
     2281 gtgcgcatat tcactgatca ggacggattg cccaacaata ttttggccgg ttttctctct
     2341 gaaggcgata cttgcctgtg gatcagcacg tttcgggggc tttgccgctt caatattcaa
     2401 aagggcaact gcgtgaattt ttttgaaacg gacggcctct cgcacaacga attcaaccga
     2461 acttccttct ttcgatcccg cgatgggcgt atgtatttcg gcggcctcaa cggagtgaat
     2521 gccttttacc ccggccctgc cttggccgac ctacgcagcc gcagctacga tgccccgttg
     2581 ttgctcaccg gctttacccg cttcgacgga gacagcctgt tttttcagag cacgggtttg
     2641 gaaaacggcg ctcccatcat cctcacccac cgcgaccgaa tgttcagttt cagttttgca
     2701 ctggcagatt ttcggcagac ctccgacaaa caattcagct atcagttgga gcattacgag
     2761 tcaaactggt cgcaggcttc cgccatcaac gaagcccgtt acaacaacat tcctgccggg
     2821 aaatacacct tccgggtgcg cgcccgttcg ggcaacaatg actggagcga tcaggagttg
     2881 gccatacctg tgctcatccg gcaggcgtac taccggagca actggttttt gggactgtgc
     2941 gcgctgctgc tgataggcgg cgcttacggc tatgcgcgct accgggttta taccctgcgt
     3001 cgccgggagc gcgtgctgga gcaattggtc ggcgcccgaa cgcaacagtt ggaaaaggcc
     3061 cgccaggaat cggagtcgct gctgctcaac atcctgcctg cgcagatcgc cgatgaactg
     3121 aagcgcttcg gcaaagcccg cgcccgcatg cacgagcagg ctacggtcat gttttccgac
     3181 ttccgagatt tttccaacat ctctgaacgg atggagccag aagccctcgt agccgaaatt
     3241 gacgcctgtt tccgcgcttt cgacgaaatc accgaccgac atggcctcga aaaaattaaa
     3301 accatcggcg atgcctacat gtgcatggta atagacaatg gcaacgaggg cgctctgcgc
     3361 agcattcgcg ccgccttgga aatacaggac ttcctgcgcc aacatgcaga gaacaaccgc
     3421 gccttctttc gaggcgcgca tcggcattca cacggggccg attgtggcgg gggtggtggg
     3481 ttccaaaaaa tttgcctacg acatctgggg cgatacggtc aacattgctt cccgcatgga
     3541 aacgcatggc gaaaccggcc aggtaaacat ctccggcgct accaaagcat tagtggaaaa
     3601 cgatatagcc tgcaccttcc gcggccgttt ttccgctcgc cacgatcggg agatcgagat
     3661 gtattttgtg gagaggtagg gtatccttac cgaatactct gcaaaaactc cgcccgcacg
     3721 ctctcctgca agaacttgcc ggaatactcc gtcgtcaccg ttgagcagtt ttcgtgttcg
     3781 gcgccgcgca attctacgca catgtgccgg gcatcgaggt aaactgccac gtcttccgtg
     3841 ttcagcgctt ttttcagttc ttcggctatc tgtatcgtca ggcgttcctg tacctgcggg
     3901 cggcgcgaga agtactccac aatgcggttc agcttcgaca aaccaatgac ctgaccggaa
     3961 gaaaaataag ccacatgcgc caccccggtg atgggtagga agtgatgctc gcaaaacgac
     4021 ttgacggtga tgttgcgctc cacgagcatc tggttgtagc ggtatttatt ttcaaaaagc
     4081 gagatgttgg gcttgttctc ggggtctaat ccggagaaga tttcctgcac aaacatcttg
     4141 gccacccggc gcggcgtacc ccgcagcgag tcgtcgctga ggtctaagcc cagggtctcc
     4201 atgatgactt tgaaatgatc ctctatgatg ttcatttttt cctcctcact tttcagaaaa
     4261 gcgtcggggc gcatgggcgt atcgaagtcg ggatcctcct ccaaaaggta gtaattgctg
     4321 tggccgttta gttttccctt agaagctgca tggtggccat tgccgttaaa agtagtcttt
     4381 gtcattgtct ttggttttgt ggtcgtacac cggctaaacc aacagaagcg gggctatatt
     4441 gttgtaattt tttcgtgccg caagcaggtt gacgcccaaa ttgccgttca aattgtggta
     4501 ctttgtcgtt gaaaaaaatc tgccgtatca tccatctctg ttttttatga aaccgactca
     4561 attgctgctc ttcgccctgc tcctcgttgc cttctctcaa tgcagcggcg gccccaaaaa
     4621 actcgaagat tgtccctacg gcaagcccaa agccattttt tcggaacaaa tgccgggcgt
     4681 gacgcgccac agctttcgca ccgacgacct ctccgcgacc gaagaactga cgctgggcga
     4741 ttcgctctcc gtcacgctca tccaaagcgg ctgcgaaaaa ccggtgcagg agtttcgttt
     4801 cgtcatccaa aatccgccgc cacaagccga tgccgcctac tgggccgacc gcggggtgga
     4861 cctgctctac caattgagca gcctcagccc ggaactcgtg ccgctctctg cctgggcgca
     4921 ggccgtcgag cagcagatac ccggcatatc gctcggcgaa cctttccctg ttgaacagga
     4981 tacttacgtc agcatagacc gaatcgagag cgccggcaat gtcattctta tgcttattct
     5041 gggcgccgaa ccctgagtaa aaacgcttgt atcggggagc ttnnnnnnnn nnnnnaaaag
     5101 aggcctcagt gccttgattt tcaggaaaat gagatttttt atgtttttat aaaaaattga
     5161 ttttcaacta aataaccaaa tggttgacac attgctcgct aaaaaaaact caaaaaaaaa
     5221 ttgcccgatt ttttggtggc agcctgcctt cgggcgtatc tttgcccaca cagaacaatt
     5281 catcacagac gctcttttta cacgatgatg ttccggcaca aagctggaac agggtgattt
     5341 ttgcgccgcc agaaacggcg tctcaataag attttttaag tatcagtaag ttagggagct
     5401 ccggcgtgtt gtgcgtcgga gtttttttct tttatacagt tttcaccgaa ccgggctatc
     5461 tttgcggcgt ttttcaacga aacaataacg catatccatt atgaatttcg atctcatcgt
     5521 tatcggcagc ggccccggcg ggtacgtagc cgccattcgg gcatctcaat tgggcatgaa
     5581 agtagccgtc gtcgagcgcg aatcgctggg cggcatctgt ctcaactggg gctgcattcc
     5641 caccaaagcc ctcctgaaaa gcgctcaggt ttttgaatac atccaacatg cctccgacta
     5701 cggcattcag gtcagcggcg ctcaggccga tttcggcgct atggtcaagc gcagtcgcgg
     5761 cgtagcagac ggcatgagca agggcatcaa cttcctgttc aaaaaaaata aaatcaccgt
     5821 catcaacggc aatggccggt tggtgcgcgg caaaaaagtg gaagtgaccg ccgccgccgg
     5881 agctaaaacc gaatacagcg ccgagcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgatgtttct aaagaattgg ccaaaattta
     6181 ccaaaaagcg ggcatcgaag tactggccaa taccagcgtg gaaaccgtgg ataccaaagg
     6241 cgctggctgt aaagtgacgg tgaagagccg caaagatggt tcggtgaagc aaattgactg
     6301 cgacgtggtg ctctccgctg ccggcgtgac gcccaacaca gaaagcatcg gactggaaga
     6361 actcggcgta aaaaccgacc gcggctttgt gctcgtggac gactactatc gcaccaatgt
     6421 acccggcatt tatgccatcg gcgacatcat tcctacccag gccctggcgc acgttgccag
     6481 cgcggaaggc attacctgtg tggaaaaaat agccggccat acgcccgatc ccattgatta
     6541 caacaacatt ccgtcctgca cctactgttc gccggaagtg gcttctgtgg gcctcaccga
     6601 gcaggccgcc aaagacgccg gctacacgct caaagtgggc aagtttccgt tctccgcttc
     6661 gggcaaggcc agtgcggcag gcgccaagca gggtttcgtg aaggtcattt ttgatgccaa
     6721 atacggagag ttcctcggcg cgcacttcat tggcgccaat gtgacggaaa tgattgccga
     6781 ggtggtggtg gctcgcaagc tggaaaccac cggccacgaa atcatcaagg ccatacaccc
     6841 gcaccctacc atgagcgaag cggtgatgga agccgccgcc gccgcttacg atgaggtgat
     6901 acatttgtag aaaaaaaacc acgcttcaga ccttgaaagg ttttagaaaa ctttgcaggg
     6961 tctgaagcta ctgcttacga cacgctccgg ctcaatcact tttgatgcca taaaactcag
     7021 ctctgatctc ataagccaag tcattcaggt agccggaatc cggtgcatcc ggtaagtcgg
     7081 aagctgcata agcggtctcc atttcatcgt atttggcggc ggccatttct ataagctgct
     7141 cataatcgta agcccctttt ttgatgctca gcaaaaagtc ggcatcgggc cttctgacga
     7201 cgatcttttt gtgccgggca atttcaatgg ccatttccag aagcctgaat gtgtgcatca
     7261 tgtttttggc atcgtagttt tttccatgat gcagggtgct ttgataacgc gcttcatttc
     7321 ggttttctac ccattcccaa tactcgcggt attcgcgaca ataaacagag aatccatttt
     7381 tgttgaagta catccatgcg acttgcggcg ctcctttggg gatggaactg agtgaaatat
     7441 catcggaatt gctcttctga aaaattcctt tgtacccact gtcttcacca taataaagcg
     7501 cgtacacgtt ttgcatgttg gaaatattga ccaagccaca gtattcctgt tgcatgttgt
     7561 tctgttgcaa aaagcgcctc agggaaatcg agccgccatt gtgggcaaca tnnnnnnnnn
     7621 nnnnagtacg gttttgcgct cccgatccat agggtttaca atttttttgt tcaaacctct
     7681 ggccttcttg acctgcgaaa gcgcaaacct gccgaaagtc tctttgcact gccgggagac
     7741 aattttcgaa cagtccactt gatggagcag tggatgtttg tatatcaaag catcggcagg
     7801 agtttccagt aactccaata tattagggtt gttcacagaa agcaattcca caaacctcct
     7861 ccattcgtaa agcatgtggt tgttgttgtc caaattgatt tgcgggatgt attgcaatcc
     7921 ataaaattgc ctgcgaggca gcatgaatac gcccctgaag tccatgtcgg actccggagt
     7981 atccaaacca taagccttgc tgccgctcaa ggcttccagt atcaacagcc tgttgtttct
     8041 caaatattgg agttccatat ccgctcaagt gtgttgataa acaattcatt acatacgtct
     8101 gatcgccctt tgccggaggg cagttgcgct ccattttcgg tagctgtgaa taaggagtgg
     8161 cgaacaaaat cgagcaagtt gctctcccca cgatgcaggt aagactcgtc agccttcgac
     8221 ttgatatcca aaagtctctc tatatccgac aaaatggttt ccggcaaatc aagcccactc
     8281 agcatatcgt tgaatgagac aggcggcggc tcattttttt ccaatatcca cctgcaagcc
     8341 gttgccgacc gcaatgcgta aaacagcttt ttgagcttgt atggtctgct ttcgtctatc
     8401 tcgtccaggg tgttccgggc catgttcagg tagtggtgca tggcgcctat gcgagagtaa
     8461 aagtcttgag aaaccgatct gatctgctcc aannnnnnnn nncctcgtca caggcgtaca
     8521 ccaccggcga atgtattctt tccaaaagtg gcggattgga gcgcagcagc aatcgcaacg
     8581 acttccgtaa atcccaaccg gacacatcca attctctgtt ttccaacatg gtatccatgc
     8641 tgtcttgttt ttccgataag gacaaatacc attgcaccgg atgggcatat atgagacgaa
     8701 catcgtagtc gctgtcggga gagggaaaac cccaggcgcg cgaaccggtt tccgaggcga
     8761 gcagtatttt tacccccctt tcagattcga cctgcttgat gtatgcgttt atttttgata
     8821 ccatgacatt tgttggaatg aaacatgcga ggaacaagcg gaccgggcaa tgtagttcct
     8881 taccatgtcc acaactcctg tgcgcgggct aactgctccg gcttgccgac atccaaccat
     8941 aagtcctgat cgtggcgaaa gccttggatg atttcgtttt gtgccaggct gaggtaagtt
     9001 tccacaatgg aaaaaacctc tccgggcggg ggcatcagct caaaaatacg ggggtgaatg
     9061 atgtgtactc cgctgaaggc taaggggtgc aagggatggt tggccaaggc aatgcgttct
     9121 gcgccggtag ctgagtttcg ccagccacag agccggttgt ttttatcaaa aagaaaagcc
     9181 ctggaggccg gtcgttcgcg tactgccagc gtagccaatg cctgagaacg ggtgtgcgcg
     9241 gcgtacaaat cattcaggtt caatgtggta aggatgtctg cattatacac caggaacggt
     9301 tggccgtcat cgaaaaacca ggcagccttg cgcaaaccgc cgccggtgtc aagcagtttg
     9361 gcgcgctcat ccgagatggc gatgttcata tctgaaaacg gaggccgcgc cagaaatgcc
     9421 gctatctgct ctccgaaatg gtgtatgttc acgatgatgt cggttatgcc tgccaagtgg
     9481 agtttctcga tggcgatttg caacagcggc acgccgttga cttcgaccaa ggctttgggc
     9541 cggtcgttgg tgagtgggcg caggcgcgtg cccaatcctg ctgcgaaaat catggctttc
     9601 atctggcaaa gatagcagag tgcaggaagg cttccaaata tggcggtttc gtcaggcgca
     9661 gttgcgcggt gaaagtgccg gatatttctt ttagggttac cgcattttta agtgttgttt
     9721 gcacaaacaa tgttccttac ctttgtgaat caggcaaaac aagcaaaaca tggctaaaag
     9781 aagacctacc atcaacgaag aagaagcgct caaaatcgtg acccgcgaag tgagcgaagg
     9841 agatgtgacc ggcggagaaa cgccctcaga ggcgcgcgtc aagtcggtta accgccgcat
     9901 ccgggccaaa atcaaccatg aagccacggc actcaaagac gctatggagt cgaagccgga
     9961 tgataaaaaa tcagcggccg agcaatacct gcaaagcttt ccggtggaag agcgcgtgtt
    10021 gttgctcaaa acgctgctgg aggaaaactt gcaaggccag ggcaaaaaag ttcatcccga
    10081 tcacgaactc gcccgtgact ggcgcgaagg cggatacccg tataaaaacc tcctttcgcg
    10141 caaagcctac gaagcgcaaa agtatgatct tcaggtagaa ctgctcaaat tgcaggcctg
    10201 gaccaaaaag accggcgccc gcgtggtggt actctttgaa gggcgcgacg cagccggaaa
    10261 gggcggcacc atcaagcggg tgatggagca cctcaatcct cgcggcgccc gcgtggtagc
    10321 tctggaaaag cccaccgaag aggaaaaagg ccagtggtat tttcagcgtt atgtgcgcca
    10381 tttgcctact ttcggcgaga tcgtgctgtt cgaccgatct tggtacaacc gggccggcgt
    10441 ggaaaatgtg atgggttttt gcaccaaaga ggaatacacg gagttcatgc ggcaagtgcc
    10501 tgattttgaa cgtaatttgg tgcgcagcgg catcattttg atcaagttct ggttctcggt
    10561 gagccgggaa gaacaaaaac gccgattcaa agaccgcgaa acacacccgc tcaaacaatg
    10621 gaaactctcg ccgatagacc gggcttcttt agacaaatgg gaagactata ccgatgctaa
    10681 agaaaaaatg tttttccata ccgatacctc cgattcgccc tggatcgtag tgaaatcgaa
    10741 ttgcaaaaaa cgcgcccggc tcaatgccat gcggtacgtc ttgcaccgga ttgcttatga
    10801 caatcgggac ttgaaaatga ttggcaaatt ggacccgctc atcgtggggc gttccaacgt
    10861 gatctacgaa aagggcgaga acttgttttt gaacgacacc ttagccaata tgctgaaata
    10921 gaggtggttg tcactctgat gctctcacaa aaccattgat ggatttgctt tgcaggtagc
    10981 gcaggtattc gtcggccgtt tgtctgctgc tgaaattgcc tatggtgatt ttgtacagag
    11041 ttttgccgtt ttgcaccgac gccgccaagc tgaccggcct tcgcaacagc ccttccaacc
    11101 ggtttacttc gttcaccgca tttccataat cgccgaacac ggcagcctgt atggtataag
    11161 tggttgccgg cccgtaatgg gtttgcgcat cggcaaccgg agacgaactg tacgacgccg
    11221 cctgcaaaac ggaaggctct atcatttcct gaacgacgtc ctgcgagtgc ggcatatcgg
    11281 gtatgagcgc cgggcgggaa aattgtccgg gagcaagcgg cgccgagttt gtttttccgc
    11341 tcatcggata ggccgggctt tcgggctgtg caggcgcaga ctgtgtccgg ggcgcttctt
    11401 cccgactgta tcgttgcagc aaggtcagaa gggcgccggc gtctatggag gcttcccggc
    11461 ggtcaatgac ttcgccttta gcattgaaaa tcaaaaccgt aggtattttt tgcacggcaa
    11521 attcctcctt gcatttcaaa ccgtag
//
LOCUS       DAORLF010000097        11422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3029_length_11422_cov_1.987508, whole genome shotgun sequence.
ACCESSION   DAORLF010000097 DAORLF010000000
VERSION     DAORLF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_11422_cov_1.987508"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK198_09755"
     CDS             <1..571
                     /locus_tag="PK198_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39062.1"
                     /translation="TCVGCHLADYNNTTNPNHLAAQFSTNCVSCHSQNSWVPSFFDHN
                     DFYPLLGAHAAIANNCTACHLGGNYNNTPNTCFGCHQQEYNSANDPNHLSAGFPTDCQ
                     TCHSVNAWTPSTFNHDGMYFPIYSGKHDGEWNSCTDCHLTPGNFSAFSCIDCHEHNNP
                     AELANDHEDVNGYIYQSSACFACHPDGED"
     gene            581..>1886
                     /locus_tag="PK198_09760"
     CDS             581..>1886
                     /locus_tag="PK198_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39063.1"
                     /translation="MKSYWTYLLFLLGWVTNSSAQNEPARMQGTVSFVSSRNVYVKFA
                     TTEAISINDTLFLDTNGALRPALQVENKSSTSTVCKPLTEQTFSIGTPIWALAPIVTQ
                     PQIPTPTTPPVERPVSEQTPPVAGNVLTPSEDAGPASRTKSKISARLSVASYSNFADF
                     KDLHRMRYAWNLSGQNIGGSKFSIENYITYRHTLNEPGSPQNSLAQALKVYAFSVRYD
                     IDSTTSLSLGRRINPRMSNIGAIDGLQAEKRLGRMLLGAVVGSRPDFVNYGLNLNLLE
                     AGAYLGWGSKQGGRYHQSTLGFMEQLNGFRSDRRFLYLQHSSRPIGQLSIFTSMEADL
                     FENINEVKKNVLRLTNVFASLRYRFSKKLELPLSYDARRNIIYYETYKNILDQLVEDE
                     SRQGVRVGISSQPFKTLSVGFNAGYRFQQSNINSSANYNAYIN"
     assembly_gap    1887..1906
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1907..2223
                     /locus_tag="PK198_09765"
     CDS             <1907..2223
                     /locus_tag="PK198_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39064.1"
                     /translation="KIGSLPIRTSLTANYLKTKYLESRIVGARSSMRVLKNKAEIEVS
                     YRNAHLRLGTLATQRQQNILGLSISANLMQHLSLSLFYEGTLDEPSQTVHRFNTRFMY
                     RF"
     gene            2272..3045
                     /locus_tag="PK198_09770"
     CDS             2272..3045
                     /locus_tag="PK198_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GW dipeptide domain-containing protein"
                     /protein_id="HRF39065.1"
                     /translation="MKNLVSAVVVFVMLVTTGCNSGPKVIESEPAEETAEGQSAGGDF
                     SFQNAPTAQASDAGQMREVTVAEKLDTERYTYLRVKEGTSEFWIAILKREVEVGQTYV
                     FKGGLLKKNFYSQEFNRQFETLYLVSEFQKKGGDGSEAELDAGFAQMASPTVKSGEIT
                     HAAGAVKLAELFANKSKYAGKEILITGKCVKINPMIMNRNWVHIQDGTGQQTDLTITT
                     TENIQPGSVVTLKGIIALDKDFGAGYKYDIIMEGAVVVQ"
     gene            3057..>4406
                     /locus_tag="PK198_09775"
     CDS             3057..>4406
                     /locus_tag="PK198_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRF39066.1"
                     /translation="MKILKLISDRLFSTSAAGLYMLLFALAIGAATFIENDFGTSSAR
                     KLVFNAWWMELLMALFGGAILVNIFRFRMVQQKKWAVLSFHAAILVILAGAAVTRYFG
                     FEGMMHIREGGQADFFLSSETYLNFEAKVNDRTFRFVEPVYFATLGNNHFEGEYLLGG
                     KEIAVEVTDFMPNPEETLMADPQGGAVLQIVIGGANGREEYYLQEGQRSNIRGTIFNF
                     TPQEAPQAFNIRLQEGKPYFTAPTVFVQTQMATQQTDTLAPGMYHPLRLRSLYSSGNQ
                     SFVFAQFTDKARVDIQSSGVKMESSSMAGIKVRVSSGGQTSEEYGMPGVEGKQRVFTA
                     GNVQLAIAYGARRIKLPFSLRLRDFILEKYPGTNSASSYASEVTLQDPRSGLVRDQRI
                     YMNHILNYGGYRFFQSSFDQDELGTYLSVNHDWWGTWISYLGYAILTIGMALTFFHKK
                     "
     assembly_gap    4407..4432
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4449..>4784
                     /locus_tag="PK198_09780"
     CDS             4449..>4784
                     /locus_tag="PK198_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39067.1"
                     /translation="MKQLNKVLIMCIFAAASARPSAVRADSPAAIPSVSIEHAQRFGL
                     LVMQDHRGRMKPMNTYAGEIVRKLSRKESLFGLNAEQIILSMVAAPNDWYQRPLIKMG
                     KHEKTKALIP"
     assembly_gap    4785..4969
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4970..>5249
                     /locus_tag="PK198_09785"
     CDS             <4970..>5249
                     /locus_tag="PK198_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39068.1"
                     /translation="PVPGHSENLWEGPQQNHQHEQNEAATPAQQFFEVYRSALQNGMT
                     SGDWSKADLLVDELSRYQIKNGRAVMPSNVKIKAEIWLNQLNIFSRLGK"
     assembly_gap    5250..5322
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5323..>5657
                     /gene="ccsA"
                     /locus_tag="PK198_09790"
     CDS             <5323..>5657
                     /gene="ccsA"
                     /locus_tag="PK198_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF39069.1"
                     /translation="FQSVWPGKILTGIFFTAFAFHLLGLGLRWYVSGRAPWSNGYESM
                     IYIGFTTTLAGIIFGRKSVGSLAATNILAATILMVAGLSWLDPEITPLVPVLKSYWLT
                     IHVSLEAGS"
     assembly_gap    5658..5680
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5681..6180
                     /gene="ccsA"
                     /locus_tag="PK198_09795"
     CDS             <5681..6180
                     /gene="ccsA"
                     /locus_tag="PK198_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF39070.1"
                     /translation="IGAINLIFMVFRTRSNADNVNRIIREMTMISEMTLIGGLVMVSV
                     GTYLGGVWANESWGRYWGWDAKETWALVTILVYAFILHMRFIPGLKSEYAFNVASLFG
                     WASVIMTYFGVNYYLSGLHSYAAGDPAPIPPSIFYAIITLTIISLLAFWRNKQYKNNL
                     NKATT"
     assembly_gap    6273..6338
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6339..>6912)
                     /locus_tag="PK198_09800"
     CDS             complement(<6339..>6912)
                     /locus_tag="PK198_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39071.1"
                     /translation="IPANALERTDPDLQDASGYNAEIGWENFAVPGLRYSVTLFQLRY
                     NNRIGSVYQTDNQGSAYLLKTNIGNSVTRGAELYAEYQSALGNHGLWSVFSSTAYMKG
                     HYLNGTLSLNNENINIAGNALETTPVWTSRNGLRLGYRGFTALVQYSVVSGHYSDAFN
                     TVEPSANGARGWTPGYQLWDVNTSLRISPQV"
     assembly_gap    6913..6949
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6950..>7988)
                     /locus_tag="PK198_09805"
     CDS             complement(<6950..>7988)
                     /locus_tag="PK198_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39072.1"
                     /translation="HYSVPLEAIERVEIISGTAALGYGAQFGGLVNYVTKAPDTTRRI
                     SFQNIATAGSYGLLSNYTSLGGKSGALTYQAYYHRRKVDGFRQDAQSSSEAFLVKTGF
                     DVSKKLRAEASLGRSTYLYRMPGPLTDAMFQADPRQSTRSRNWYSPDIYLPALKLEWK
                     LNEETQVSWLSSAVLGTRNSVMIVGFANVPDAIDPALNQYKNRQVDIDRFNSYTHELR
                     LRHDYSLAGKWKSAFVGGVQIMDNDLHRRQLGKGTTGSDYDLTVIGNDWGRDMHFRTK
                     NLAVFAENLIYLFPKLSITFGARYESGASERSGYVRYLNPNDVPLTLQRNFVLAGASA
                     QYQLQPGHRLFG"
     assembly_gap    7989..8049
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8050..8400)
                     /locus_tag="PK198_09810"
     CDS             complement(<8050..8400)
                     /locus_tag="PK198_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39073.1"
                     /translation="MKYLYLSLAFGMLLFSSVGAQNDSVRIVDLKAVQVKSKPAASPL
                     IKSLPTLHGTSITSGRRNAILQVADMPLNYAQKNAQQVFAKVPGAMVYDMDGAGNQIN
                     IALRGLDPHRSWEMN"
     gene            complement(8500..9861)
                     /locus_tag="PK198_09815"
     CDS             complement(8500..9861)
                     /locus_tag="PK198_09815"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF39074.1"
                     /translation="MSRMLLRYKALGMMLAVLLVVSSCRKDDNNVVDTTNQLSKFQAS
                     SAWVWNEMFLEVERYADGYRPGPAPRALGYMGLSAFEACQPGMADDYNSIAELYPGLR
                     LPTFDASQEYHWPTVVNGSYAYLMKRFFPHVAADLKFKIHQLESRLDEEALNRISREV
                     FTRSKQHGEAVARAVYEWSGTDPIGHNAFLNARPISYVPPTGTGLWQPTYPDYGRGMF
                     PYWGNARTFAISESDKLCRPPVPISEAPGSALWNQAKQVHDLNTPTLAFEEQWIAEFW
                     SDDDLNLTFSPPSRWIAVADQVIDHEDASLDLALYTYLKVGLAINDAGVAAWHSKYHY
                     NIERPVSYIRRVMDPNWDTHHPYTPSFPAYPSGHATFGAAAAEVLSDIYGNNYAMIDR
                     CHEGRTDFLGMPRSFNSFKEMAEENGYSRLPLGVHFLMDATSGVELGYSVGAKVNQLR
                     IKK"
     gene            10202..>10437
                     /locus_tag="PK198_09820"
     CDS             10202..>10437
                     /locus_tag="PK198_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39075.1"
                     /translation="MRTVFVFFLLLALAFPGRADVEIEYAVVPNKQLPQVLHFLLPQR
                     ILVGINTRGDVVVRGEKPSEDAHSYNLYPVGKPY"
     assembly_gap    10438..10490
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10491..>11055
                     /locus_tag="PK198_09825"
     CDS             <10491..>11055
                     /locus_tag="PK198_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39076.1"
                     /translation="ATQVFYPDDPPVQIAGLPCRRAVSIIGKDTSDIYYTTSFGVDFC
                     PTAEIRGFAMLFTRVIHGIPVLYQAQRFQACTLPSEWFDLSKRNIERFRPGRPLDLDA
                     LEQKMSGRRAASISGTPVNRSPIKPKMLAGKVVVLSFWYSESMACLEQIPIMERMAAR
                     YAGRDNFVFLAVMPESDYLISRLLYRLK"
     assembly_gap    11056..11190
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2670 a         2699 c         2778 g         2596 t
ORIGIN      
        1 cacttgcgtg ggatgccatt tggcggatta caacaacacg accaatccca accacttagc
       61 agcacagttt tctaccaatt gtgtgagttg ccattcgcag aacagctggg ttccatcgtt
      121 cttcgatcac aatgattttt atcccttgct cggtgcgcat gccgccattg ccaataactg
      181 cacggcctgt catttagggg gcaattacaa caatacaccc aatacgtgtt tcggctgcca
      241 ccaacaggaa tacaacagcg ccaatgatcc caatcacctc agcgcaggct tcccgacgga
      301 ctgtcaaacc tgtcattcgg tgaatgcctg gacgccatct acatttaatc atgacggcat
      361 gtacttcccc atttacagtg gtaagcatga tggcgagtgg aacagttgca ccgactgcca
      421 tttgacgccg ggtaacttta gtgcattcag ttgtattgac tgccacgaac acaataatcc
      481 tgcagaactg gccaatgacc acgaagacgt aaacggatac atttatcaaa gcagtgcatg
      541 ttttgcctgc catcccgacg gagaggatta aacgaatgtg atgaagtcat actggacata
      601 tctgttgttc ctgctgggtt gggtgacgaa ctcctcagcg cagaatgagc ctgcccgcat
      661 gcagggtacc gtcagttttg tatcctcccg gaatgtatat gtcaaatttg ctaccaccga
      721 agcgatttcc ataaacgata ccttgttttt ggataccaat ggggctttgc gcccggcatt
      781 gcaggtagaa aataaatctt ccacttcgac ggtgtgcaaa ccgctgacgg agcagacctt
      841 ttcgatagga actccgattt gggcgctcgc tccgattgtt acacaaccgc agataccgac
      901 tccgactacc ccacccgtcg agcggcctgt ttccgagcag acacctcctg tggccggcaa
      961 cgtcctcacg cccagtgagg atgccgggcc ggcgtctcgt accaaaagta aaatttctgc
     1021 gcgcttgtct gtggccagtt acagcaattt cgccgacttc aaagacttgc accgcatgcg
     1081 ttacgcctgg aacctgagcg ggcaaaacat cggcggctct aagttttcca tagaaaatta
     1141 catcacctat cgccatacgc tcaatgaacc cggcagccct cagaacagtc tggctcaggc
     1201 actgaaagta tacgcctttt cggtgcgata cgacatagat tctacaacct cgctcagctt
     1261 gggccgtcgc atcaacccgc gcatgagcaa tatcggcgcc atagatggcc tacaggcaga
     1321 aaagcgcttg ggcaggatgc tgctgggagc cgtggtggga tccaggccgg attttgtaaa
     1381 ttatggcctg aatctcaact tgttggaagc aggcgcctac ctcggatggg gatcgaagca
     1441 gggaggaagg tatcaccaat ccaccctggg atttatggaa caactcaacg gattcaggtc
     1501 agaccgaagg tttttgtatt tgcaacacag cagccgtccc attgggcaac tgagcatatt
     1561 tacctctatg gaggctgacc tgtttgaaaa catcaacgaa gtgaaaaaaa acgtgctgcg
     1621 actgaccaac gtcttcgcat cattgaggta tcgtttttcg aaaaagctgg agttaccact
     1681 gtcttatgat gcccgccgca acatcattta ttatgaaacc tacaaaaaca tactggacca
     1741 attggtagag gacgaaagtc gccagggtgt tcgggtgggc atcagtagtc aacccttcaa
     1801 aacgctgagt gtgggcttta atgctgggta tcgtttccaa cagtcgaata tcaattccag
     1861 tgccaactac aacgcctata tcaaccnnnn nnnnnnnnnn nnnnnncaaa gataggttcg
     1921 ctgcccatca ggacttcact gacggccaac tatctgaaaa ccaaatatct ggaaagtcga
     1981 attgtaggtg cgcgatcatc catgcgggtg ttgaaaaaca aagctgagat tgaagtatcc
     2041 taccgcaatg cccatctccg cctcggcact ttggctacgc agcgccaaca gaacattctt
     2101 ggcctgagca tttcggccaa tttgatgcaa cacctcagct tgtccctgtt ttacgaaggc
     2161 acgctcgatg agccttcgca aaccgtacac cggttcaata cccgtttcat gtatcgtttt
     2221 tagttgctga gtatgtacct ttgttttcgt ttcaaaataa aaattcagat catgaaaaac
     2281 cttgtaagtg cagtagtagt gtttgtaatg ctggtgacta caggctgcaa ctcgggacca
     2341 aaagtaattg agagtgaacc ggcggaggag acggcggaag ggcagagtgc aggcggagat
     2401 ttttcatttc agaacgcccc gacagcacag gccagtgatg ccggacaaat gcgtgaagtc
     2461 acagtagcag aaaaacttga cacggagcgt tacacatacc tgagagtaaa agaaggcact
     2521 tccgagttct ggatcgccat tttaaaaaga gaggtggaag tagggcagac ttacgttttc
     2581 aagggcggct tgctgaagaa aaatttttac agccaggaat tcaaccgaca gtttgagaca
     2641 ctgtacctcg tttcggaatt ccagaaaaaa ggcggagatg gcagcgaagc cgaattggat
     2701 gcgggattcg ctcagatggc ctctccgact gttaagtccg gcgagatcac tcatgctgct
     2761 ggtgctgtca aattggccga attgtttgcc aacaaaagca aatatgcagg taaagaaatt
     2821 ttgataaccg gtaagtgtgt caaaatcaac cccatgatca tgaaccgcaa ctgggtgcat
     2881 attcaggacg gtaccggcca acaaacagat ttgaccataa cgaccactga aaacatacag
     2941 ccaggcagcg tggtcaccct caaaggcatc attgcattag acaaggactt cggcgccggc
     3001 tacaagtatg atatcatcat ggaaggagct gttgttgttc aataacctac tttgcaatga
     3061 aaattctcaa actcatttcc gaccgcctgt tttccacttc ggctgccggt ctgtacatgt
     3121 tgttgtttgc cttagccatt ggcgccgcca ctttcattga aaacgacttt ggcaccagtt
     3181 cggcccgaaa gctggttttt aatgcctggt ggatggaact gctcatggcc ttgtttggtg
     3241 gcgctatttt ggtcaatata ttcagattca ggatggtgca acaaaaaaaa tgggcagtgc
     3301 tgtcctttca tgcagccata ttggtcattt tggcgggggc agcagtgaca cggtattttg
     3361 ggtttgaagg catgatgcac attcgggagg gagggcaagc cgactttttt ctgtcttctg
     3421 aaacgtatct gaactttgag gcgaaagtga atgatcggac gttccgattt gttgaacccg
     3481 tatattttgc gacgctgggc aataatcatt tcgaaggaga gtatttgttg ggcggcaagg
     3541 aaattgctgt agaagtgacc gactttatgc ccaacccgga agaaacgctg atggcggatc
     3601 ctcagggcgg cgcagtgctt cagatcgtga tcggcggcgc gaacgggcgg gaggagtact
     3661 atcttcaaga gggccaacga agcaatatcc ggggtacaat tttcaacttc acaccacagg
     3721 aagcccccca ggctttcaac attcgtttgc aggaaggcaa accctatttt acagcgccca
     3781 ctgtttttgt tcagacacaa atggccaccc aacaaaccga tactctggcg ccgggcatgt
     3841 atcacccact gcgtttgaga agcctgtata gcagtggtaa tcagagtttt gtgtttgctc
     3901 aatttacaga caaagcacgc gtggacatac aatcatccgg cgtgaaaatg gaaagctcca
     3961 gcatggccgg cataaaagtc cgggtgagtt caggtggtca gacatcagaa gaatacggca
     4021 tgccgggcgt agaaggcaaa cagcgcgttt ttaccgccgg caatgtacaa ttagccatag
     4081 cttatggggc gcggcgcatc aaattaccgt tttcgctccg cctccgcgat ttcatcctcg
     4141 aaaagtaccc cggcaccaac agcgcctctt cctatgccag cgaagtgacc ctgcaagacc
     4201 cgcgctccgg gctggtaagg gatcagcgca tctatatgaa ccacatcctc aactacggag
     4261 gctaccgatt ttttcagtct tctttcgatc aggacgaact gggcacttac ctcagcgtca
     4321 accatgactg gtggggcacc tggatttcct atctcggcta tgccatcctc accatcggta
     4381 tggcgcttac tttttttcat aaaaaannnn nnnnnnnnnn nnnnnnnnnn nncgaaaatc
     4441 taaaagccat gaagcaactg aataaggtgc tgattatgtg tatattcgct gccgcatctg
     4501 ccaggccttc cgccgttcgc gcagacagcc cggccgccat tccttcggtt tccatcgagc
     4561 acgcccaacg cttcggcttg ctggtcatgc aagatcatcg cgggcgcatg aagcccatga
     4621 atacttacgc cggtgaaata gtgcgcaaac tatcccgcaa agaatcgctg tttggcctca
     4681 atgccgagca aatcatcctg agcatggtag cggctccaaa cgattggtac caacgcccgc
     4741 tcattaagat gggcaaacac gaaaagacga aagccctcat tccgnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccggttcccg
     4981 gccactctga aaacctgtgg gaaggccctc aacaaaacca ccagcacgaa caaaacgagg
     5041 cggccacacc tgcacagcaa tttttcgaag tatatcgctc ggcgctgcaa aacggtatga
     5101 cctccggcga ctggtcgaaa gccgatcttc tggtggacga attgagccgc tatcagatca
     5161 aaaacggtag agcggtaatg ccctccaatg tcaaaatcaa agcagagata tggctcaacc
     5221 aactcaatat ttttagtcgg ctggggaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctttcagt cggtttggcc
     5341 gggtaagatc ctgacgggta tattttttac agcttttgcc tttcatctgc tgggtttggg
     5401 gcttcgctgg tatgtttccg gtcgtgcgcc ctggagcaac ggctacgagt ctatgattta
     5461 cattggtttc accactacat tggcgggcat catattcgga cgcaaatcgg tgggaagttt
     5521 ggccgcaaca aacatcctgg cagccactat cctcatggta gcgggtttga gctggctcga
     5581 tcccgaaatc actccgctgg tgcccgtact gaaatcctac tggctcacca ttcacgtttc
     5641 gttggaggcc ggcagctnnn nnnnnnnnnn nnnnnnnnnn ttatcggtgc catcaatctg
     5701 atttttatgg ttttcagaac ccgatccaat gccgacaatg tgaaccgcat catccgcgaa
     5761 atgaccatga tcagcgaaat gacactcatc ggcgggctgg tgatggtcag tgtgggtact
     5821 tatctgggtg gggtttgggc caatgaatct tgggggcgct actggggctg ggatgccaaa
     5881 gaaacctggg cgcttgtcac tattctggtc tatgcattta tattgcacat gcgtttcatt
     5941 cccggcctca aaagcgaata cgcgttcaat gtagcctctc ttttcggatg ggcctctgtc
     6001 atcatgacct actttggcgt caattactat ttgtctggtt tgcattctta tgcagcaggc
     6061 gacccggcgc ccattccgcc atctattttt tatgcgatca ttaccctgac catcattagt
     6121 ttactggctt tttggagaaa caaacaatac aaaaataatc tgaacaaggc tacaacctga
     6181 tctcgctcgg atgctcaaaa cgtagtctcc aacgagataa aataattccg cccgtcgctg
     6241 ctccaaacac ccggccccgg atatccggcg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ttgagggctg atgcgcaacg
     6361 aagtattcac atcccacagt tgatagcctg gtgtccagcc gcgcgcgccg ttggcgctgg
     6421 gttccacggt attgaaggca tccgaataat gcccgctcac cacgctgtat tgtaccaatg
     6481 cggtaaaacc tctgtacccc agtcgaagac cgttgcggct ggtccatacc ggtgtggttt
     6541 ccagtgcatt gcctgcgatg ttgatgtttt cattgttcaa actcaaggtg ccgttcaggt
     6601 aatggccctt catgtatgcg gtggagctga aaaccgacca aagaccatga ttgccgaggg
     6661 cactctggta ctctgcgtac aactcggcgc cgcgcgtgac ggagttgccg atgttggttt
     6721 tcagtaggta cgcactgcct tgattgtcag tttgatacac gctgccgatg cgattgttgt
     6781 atctcagttg aaacaaagtg acggaatagc gcagccccgg cacggcaaag ttttcccaac
     6841 caatttcggc attgtagccc gatgcgtcct gtaggtcggg gtcggtgcgt tccagcgcat
     6901 tggcgggaat gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccaaataaac
     6961 gatgtcccgg ctggagttga tattgcgcgg aagcgcccgc caaaacgaaa ttgcgttgca
     7021 gtgtcaaagg cacgtcgttg gggttcaggt atctgacgta cccgctccgc tccgatgccc
     7081 ccgattcgta ccgggcgccg aatgtgatgg aaagtttggg aaacagataa atgagatttt
     7141 cggcaaaaac ggccagattc ttggtgcgga agtgcatatc ccggccccag tcgttgccta
     7201 ttacggtgag gtcgtagtcg cttccggtag tacccttgcc caattgccgc cggtgcagat
     7261 cgttgtccat gatttgcacg cctcccacga aagccgattt ccatttgccg gccagcgaat
     7321 aatcgtgccg cagccgcagt tcgtgggtat aactgttgaa gcgatcaatg tccacctgcc
     7381 tgtttttgta ctgattgagg gccgggtcaa tggcatcggg cacattggca aagccgacaa
     7441 tcataacgct gttgcgcgtg ccgagcaccg ccgaagacag ccaggatacc tgggtttcct
     7501 cattgagctt ccattccaat ttcagcgccg gcaggtaaat atcggggctg taccagttgc
     7561 gcgaacgggt ggactgccgg ggatcggcct ggaacatcgc atcggtaagc gggccgggca
     7621 tccggtacaa ataagtggag cgacccagag atgcttcggc acggagtttt ttgctgacgt
     7681 cgaagcctgt tttcactaaa aacgcttcag aagaagactg tgcatcctgc ctgaaaccgt
     7741 ccacttttct gcgatggtag taggcctgat aggtgagcgc gccggatttg ccgcccagcg
     7801 atgtgtagtt gctcaacagc ccgtaagaac ccgcagtggc aatgttttga aacgaaatcc
     7861 tgcgcgtggt atcgggggct ttggttacat aattcaccaa tccgccaaac tgcgctccgt
     7921 agcccagcgc cgccgttccg ctgatgatct ctacccgctc aatggcttcc aacggcacac
     7981 tataatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnna ttcatttccc aggaacggtg tggatcgagg ccgcgcagcg caatgttgat
     8101 ctgattgccg gcgccgtcca tatcatacac catggcgccc ggcactttgg caaacacctg
     8161 ttgggcgttt ttctgggcat agttgagcgg catgtcggcc acttgcaaaa tggcgttgcg
     8221 gcgtccggag gtaatgctcg tgccgtgaag cgtgggcagg ctttttatta aaggagaggc
     8281 ggccggcttg gatttgacct gtacagcttt cagatcaact atcctgacag aatcattttg
     8341 ggcgccgaca gaagagaaaa gaagcatgcc gaaagccaaa gatagataca agtatttcat
     8401 ggttttttga gttggttgta caaaaaaatg agccatcccc ggtgcaggaa tggctcataa
     8461 gaaggactaa aatatggccc cttccgggta aaaaaagaat tacttcttga ttctgagttg
     8521 gttgacttta gcacccacgc tgtagccgag ttccacaccc gatgtagcat ccatgaggaa
     8581 gtgtacgcca agcgggaggc ggctgtatcc gttttcctca gccatttctt tgaaagaatt
     8641 gaaactgcgc ggcataccga ggaagtcggt gcggccttcg tggcagcggt caatcatggc
     8701 gtaattgttg ccatagatgt cgctcagcac ttcagcagca gcagcaccaa aggtagcgtg
     8761 gccggaagga taggctggga agctgggcgt gtacgggtga tgtgtgtccc agttggggtc
     8821 catcacgcgg cggatataac tcaccggacg ctcaatgttg tagtgatact tggagtgcca
     8881 agctgccacg ccggcatcgt tgatggcaag acccaccttg agataagtgt aaagtgcaag
     8941 atcaaggctg gcatcttcgt ggtcaatgac ctgatcggca actgcaatcc agcggctcgg
     9001 cgggctgaat gtcaggttaa ggtcgtcgtc gctccagaac tcggcaatcc actgctcttc
     9061 aaacgccagt gtaggtgtgt tcaggtcgtg tacctgcttg gcctggttcc aaagagctga
     9121 acccggcgcc tcgctgatgg gtaccggcgg acggcaaagc ttgtcggact ccgaaatggc
     9181 aaatgtgcgc gcattgcccc aataagggaa catacctctg ccgtagtcag gataggtagg
     9241 ctgccacaaa ccggtgccgg tgggcggaac gtagctgatc gggcgggcat tgaggaaggc
     9301 attgtgcccg atgggatcgg tgccggacca ctcatataca gcgcgggcga cagcttcacc
     9361 atgctgcttg gagcgggtga atacctcgcg ggaaatgcgg ttgagcgctt cctcgtcgag
     9421 gcgagactcc aactgatgga ttttaaattt cagatcggcg gcaacgtgcg ggaaaaagcg
     9481 cttcatcagg taagcgtaag aaccattcac aaccgtcggc cagtggtatt cctgacttgc
     9541 atcaaaagtg ggaagacgca aacccggata caattcggca atggaattgt agtcatcggc
     9601 catgccgggc tggcaggctt cgaaggccga aagccccata tagcccaatg cgcgtggcgc
     9661 agggccggga cggtagccgt cggcgtagcg ctctacttcg aggaacattt cattccagac
     9721 ccaggcagag cttgcctgaa atttgctgag ttggttggtg gtgtcaacga cgttgttgtc
     9781 gtctttgcgg caagaggaaa cgacgagcaa aacagccaac atcattccca gcgctttgta
     9841 gcggagtaac atcctgctca tggttggtac aatttgttag tgaaaaaaag attggtttga
     9901 tggatacaaa tgtaatggtc tccaagttga gacgccgcca acagtaatca gacacaaaat
     9961 gggaatggga atcaaaaagg ctttgatcgc tcctaaaaga agtgcctatg ccgacaagca
    10021 acataaaacc ggaaacgaac acttggtata agtagtgtag cctttgtgcc ggcaaatcta
    10081 aatacatttg tgtaatggtg gcaacatttt aacatttgaa aattcaaaaa aaaatggctg
    10141 atatttataa aaaattgttt ttcaaatgaa tatgaatttt atttttgaaa aaaaaatcct
    10201 catgcgcact gtttttgtat tttttctctt gcttgcgctc gcattcccgg gccgcgccga
    10261 cgtagaaatt gaatacgccg ttgttccaaa caaacagctt ccgcaggtat tgcacttctt
    10321 attgccgcaa cgcattcttg tgggcatcaa cacccgcggg gacgtagtgg ttcgcggcga
    10381 aaagccatct gaggacgccc attcgtacaa tttgtatccg gtcggaaaac catattgnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccacacag
    10501 gtcttctacc ccgatgatcc gccagtgcaa attgcgggtt tgccttgccg gcgggcagta
    10561 tccatcatcg gaaaagatac cagcgatata tattatacca cttcttttgg tgtggacttc
    10621 tgccccactg ccgaaatccg gggctttgcc atgctgttta cccgcgtcat tcatggcatt
    10681 ccggtactgt atcaggcgca gcgatttcag gcctgtaccc tgccgtccga gtggtttgat
    10741 ctgtctaaac gaaatattga acggttcagg ccgggccgcc cgcttgatct ggatgccctc
    10801 gaacagaaaa tgtcgggacg aagggccgcc tccatttccg gtacgcccgt caatagatcg
    10861 cccatcaagc ccaaaatgct ggccggcaag gtggtggtgc tgagtttttg gtactccgaa
    10921 agtatggcct gtttggaaca aattccgatc atggaacgca tggccgcacg ttatgccggg
    10981 cgcgacaact ttgtatttct ggcggtgatg cctgaatcgg actacctgat ttcgagactt
    11041 ttatatagat tgaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggatcgct tagaggccgt tttgcaatgg
    11221 gccatgaagc aggagcggca ggcgtgagtg gggaggtagt gtataatttg aaatttgcat
    11281 gagctatttt tgaaatttac atgaaccatt tttgaaattt gcataagcgc tatttgaaat
    11341 ttacataaac ctttttttaa aacctccccc caatgggaaa cctgatttct ttacggaatc
    11401 aagccaacag ccagcagcta at
//
LOCUS       DAORLF010000098        11403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3037_length_11403_cov_2.034896, whole genome shotgun sequence.
ACCESSION   DAORLF010000098 DAORLF010000000
VERSION     DAORLF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_11403_cov_2.034896"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1108
                     /locus_tag="PK198_09830"
     CDS             233..1108
                     /locus_tag="PK198_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRF39077.1"
                     /translation="MLLSMTGYGRETQTYLDKNISVEIRSLNSKFTDIKMRLPQKYKD
                     RENDLRRLLAERAERGKIEFALEIKSLQGDDDFALNVPLFKRYVVELRKLADELQMPS
                     GDIIPAVLRLPNVVSTEAANLDEAEWAAVMEVTNKAVQQFDAFRLAEGQAMESDLRLR
                     VSNIMTALPLVDPHQEERVVRVRQRMYQNLEEYLGKEKIDENRFEQEIIFYLEKMDIT
                     EEKVRLEQHCNYFLEELNSPDTAKGRKLSFISQEMGREINTLGAKAYSSEIQRLVVNM
                     KDELEKIKELVANTI"
     gene            1105..>1645
                     /gene="gmk"
                     /locus_tag="PK198_09835"
     CDS             1105..>1645
                     /gene="gmk"
                     /locus_tag="PK198_09835"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRF39078.1"
                     /translation="MNKMLIFAAPSGSGKTTIVRHLLSVFPQLSFSVSAATRERRAHE
                     VHGRDYYFISQEEFRQRIDNKEFAEWEEVYPGQFYGTLHSELERLWSEGKDIIFDIDV
                     YGAVRLKKKYPEQALTVFVKPPSRDVLVERLKGRGTESEETFRKRIVKAEEELAFENK
                     FDVVLVNDVLEAAFARAEEI"
     assembly_gap    1646..1680
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1725..2111
                     /gene="apaG"
                     /locus_tag="PK198_09840"
     CDS             1725..2111
                     /gene="apaG"
                     /locus_tag="PK198_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRF39079.1"
                     /translation="MDTLITNDVRISVEAFYQPNYSKPDVGEFVFAYRITIENLNSFT
                     VQLLRRHWHIFDSAGERREVEGEGVIGKQPILQPGGVHQYVSWSNLSSEIGSMQGTYQ
                     FRRTDTDEMFDVDIPQFRLITPYRLN"
     gene            2133..3134
                     /locus_tag="PK198_09845"
     CDS             2133..3134
                     /locus_tag="PK198_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39080.1"
                     /translation="MHTHILPERLPNFAEKFGYGDFIHLVHHQPGRAQMMKGDTFFRE
                     IEANCWDPELRIREYAQHRTQVQVVCTIPVMFSYWAEPDDCLDLSRFLNDHIAQLVHD
                     YPKNYIGLGTVPMQDADTAIRELQRLKDMGIVGIQIGSNINDRNLNEPEFFPIFQACQ
                     DLSLAVMVHPWNMMGEADMRRYWLPWLVGMPAETSRAACSMIFGGVLERLPELRVCFS
                     HAGGSFLPTIGRIQHGYNCRPDLVAIDNPKPPTDYLGKFWVDSITHDPVMLRYVLDMV
                     GEDKVTLGSDYPFPLGDLEIGAFIEEMGLPETTVQKIFCENSLNWLGLQKNAQLYSV"
     gene            3185..4357
                     /locus_tag="PK198_09850"
     CDS             3185..4357
                     /locus_tag="PK198_09850"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF39081.1"
                     /translation="MEAYIVKAYRSAVGKAKKGGFRNYRSDDLAVDVVKYLFDHTPEL
                     DPAMVDDVIVGCANPEGEQGLQIGRQISLRALGKEVPGMTVNRYCASGLETISIAVAK
                     IRAGMGHVYVAGGTENMSMIPMTGYKLAPSYAVAANTPDYLVGMGLTAEAVANKYGIS
                     RQAADEFSYQSHLKAARAIEEGRFSEQIVPVKVKEVFVKDNKRVETESVVAMDEGVRR
                     DTTVEGLAQLRPAFAANGIITAGNASQTSDGAAFVLVVSEDIVKQYNLQPIARMVSCA
                     VAGVEALYMGIGPVAAIPKALAQGGLKLSDIQQIELNEAFAAQALAVIQEAGLDPEIV
                     NVNGGAIALGHPLGCTGAKLSTQLFHEMRRRGQKYGMVTACVGGGQGIAGIYELLN"
     gene            4374..5249
                     /locus_tag="PK198_09855"
     CDS             4374..5249
                     /locus_tag="PK198_09855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF39082.1"
                     /translation="MISVLIPVYNFDVRPLVESLAGQCLALESPWEILCLDDGSTAEF
                     RALNRALQAIPGVRYEELPQNVGRSAIRNRLAEMASFPYLLFMDCDSAVVRPDYMAQY
                     AAHCRPDAVLCGGRVYASDPPENAAFLLHWTVGKSREEWSAARRSVQPWHGFMTNNFL
                     IPQKILLSIRFDERLREYGHEDTLFGLELERRQIPVLHLDNPLEHIGLEPAAVFLQKS
                     EQAVQNLRRLRELGIVVPTRLVSISAAVERAGMRRLVLFLLRRLEARSRRNLLSERPV
                     PAYLDALKLRWMLGK"
     gene            5306..5662
                     /locus_tag="PK198_09860"
     CDS             5306..5662
                     /locus_tag="PK198_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39083.1"
                     /translation="MLSFKVLYESLMEERSMKKKQLKIEDFSPSLFWDVDKTTLDFEK
                     NAIHVVEKALSRGSWQDFKTALNYYGRGRMTELIQKIRYLDERTTQFVSVYFQIPLTE
                     LRCYTWSRSNPSHWDY"
     gene            5659..6228
                     /locus_tag="PK198_09865"
     CDS             5659..6228
                     /locus_tag="PK198_09865"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRF39084.1"
                     /translation="MKSLMSRKYLDQFVLVGGTALALQIGHRKSVDLDMFTVSDIDTD
                     ALLENLQQDYSVQPKVRTQGSLISDIEGIKVDFIRFKYAFTYPIEEIDGIRLLSVKDI
                     APMKLDAITGRGSKKDFYDLYFLLERFTLPEILELYLQKYPHSTLFHVIKSLTWFEDA
                     DPQALPDVLEKKNTWARVKKRIEKAVAKL"
     assembly_gap    6365..6382
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6735..6767
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6768..7490
                     /locus_tag="PK198_09870"
     CDS             <6768..7490
                     /locus_tag="PK198_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF39085.1"
                     /translation="GRDEIVESATGFILEDNSKFYLITNWHVIANRNPTDSTANKIKV
                     KNAVGNELEIDVSDPEKLLIYFHGKTLGEWIPITIDLYDKGRKKWLEHPFGKKVDVVA
                     LPINLTDEQKNKIHLYSLKFDEYNDEIVKYPSINLSIIGFPFGFSSYGRFPIWKTGQL
                     SSDFDLNFDDLPIFLIDASTRSGMSGSPVILRQVGAIYTKQVVGMGGYVQEFLGIYSG
                     RIGNDSDIGIVWKIECLKEIIK"
     assembly_gap    7695..7756
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7765..>8057
                     /locus_tag="PK198_09875"
     CDS             7765..>8057
                     /locus_tag="PK198_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39086.1"
                     /translation="MNISIFQNGLLLLALSIFLLSSCGNKSQSADAAAQPEVRISETS
                     AILTKFNFEHFGSYDLSTWKLMPKVHQGDVVLEVTTYTKDGSKMDVKESDMGEG"
     assembly_gap    8058..8085
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8086..8351
                     /locus_tag="PK198_09880"
     CDS             <8086..8351
                     /locus_tag="PK198_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39087.1"
                     /translation="YELTGPDGKVLKTRDLSLTTESDAESEKLPVTITETVRDYTVNP
                     PKQFERIQKTGKLHRELKPLPTAATGAWVESTAEAYFYPEDNN"
     gene            complement(8420..>9565)
                     /locus_tag="PK198_09885"
     CDS             complement(8420..>9565)
                     /locus_tag="PK198_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRF39088.1"
                     /translation="KFDVYTLQSNAWFEEGARRATADLIQYDQKADKTFLKGKAHLVD
                     ADQDIRSEEIIYDGKKKTYATRGRSRIADPPQILEADEVDFDEEQGLGIATGNVSWQD
                     TSANLNIVCERAAYNRETGFIKASGARANRPLLRSLLEGDTLYMTADTLLSLRLDTLQ
                     GDSSRALFAYNDVRILKNDLQVICDSLSYSSVDSIFRLYKAPFIWSDTTTQFSADTMH
                     LELVDNKINRIFLFGNGLIINSPDERFFNQIKGRNITARFEEKELRVMDVSGNAQSIY
                     YARDDAGAYIGANKTECSDMVLHFGNNQIKRIKFLAEPKSKMAPMQQADHQTLRLEGF
                     IWDDIKPCRPMVLDDLFTPVCKRALPAPSVPIAPRPTVNNRSSSARE"
     assembly_gap    9566..9595
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9596..10323)
                     /locus_tag="PK198_09890"
     CDS             complement(<9596..10323)
                     /locus_tag="PK198_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRF39089.1"
                     /translation="MIIKTRQRIVYRFLFIFIWAVVMPAMLFGQEKKSNKPVDIDFAR
                     QWSLLQQRGKVLQKLVGEVQLHQDSVYMYCDSATIEDRVRVIAVGKVSIQQGDTAVIF
                     ADSLVYLSELRQAELFGNVVLVNGEQKLFTDKLHYDLNTKVATYVDGALLTNGKSKLK
                     SRRGTYFVQQKEVYFKEKVEVNDPDFTLKSDTLKFNVETKTAFFLGPTIIATGESKVY
                     TESGFYNTQTNFAEFNQNAQFVKA"
     gene            10671..11144
                     /locus_tag="PK198_09895"
     CDS             10671..11144
                     /locus_tag="PK198_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRF39090.1"
                     /translation="MSDKLDKIDLKILRILQENSKITNLDLSKKIGLSPAPTLERVKK
                     LEQTDVIESYHAKVTPQSIGLNVKTFVLVSLAWKKENALNQFLEKIMEIDEVTECYII
                     TGEADFLMKVVCKDIPTYEQLLFKTLSQIDEIERLRTLMTLSTVKDSKILPYKYE"
BASE COUNT         2827 a         2836 c         2863 g         2671 t
ORIGIN      
        1 aaattccgcg aaccacgcct gtcgtcgtgg cgaggagcga gcgaaccgcg gtttgcaccg
       61 cgccatggcg cggttgcagc cgttccaacc cgaaacctta aacccgaaac cccgccttcg
      121 ccaaggctac ggcggatgaa acccgaaacc ttgaacccca aacaaaattc cccgcgcagg
      181 ttagcccgcg aaagcctata tttgccgctc gcatcatcaa aaacgcgccg ctatgctgct
      241 ttccatgacc ggttatggcc gggaaactca aacgtattta gacaaaaata tttcggtaga
      301 aatacgctcg ctcaacagca aattcaccga catcaaaatg agactgcccc agaaatacaa
      361 agaccgtgag aacgatctgc gccgtctgct ggccgagcgc gccgagcggg ggaaaattga
      421 atttgcgctg gaaatcaagt ctttacaagg cgacgacgac tttgcactga atgtgcccct
      481 gttcaaacgc tatgtagtgg aattgcgcaa attggccgac gaactgcaaa tgccttccgg
      541 cgacatcatt cccgccgtac tgcgcctgcc caatgtggtc tccaccgaag cagccaactt
      601 agacgaagcc gaatgggccg ccgtcatgga ggtgacgaac aaggccgtgc aacaattcga
      661 cgctttccgc ctcgccgaag ggcaggccat ggaaagcgac ctccgcctgc gcgtgagcaa
      721 catcatgacg gccctgcctc tggtggaccc gcatcaggaa gagcgcgtgg tgcgcgtgcg
      781 gcagcgcatg tatcagaatc tggaagagta cctcggcaaa gaaaaaattg acgagaatcg
      841 cttcgagcag gaaatcattt tttacctcga aaaaatggac atcaccgagg aaaaagtgag
      901 gctcgaacaa cattgcaact actttttgga agaactcaac tcgcccgata ccgccaaagg
      961 acgcaaactc agcttcatca gtcaggagat gggccgcgag atcaacacgc tgggcgccaa
     1021 ggcttattct tccgaaatcc agcgtttggt ggtcaatatg aaggacgaat tggagaaaat
     1081 taaagagctg gtagccaata cgatatgaat aagatgctca ttttcgcagc gccatcgggg
     1141 tcgggtaaaa ccaccatcgt gcggcatctg ctcagtgtgt ttccgcagct ttccttttcc
     1201 gtatcggcgg ctacccgcga gcgccgggca cacgaagttc atggacgcga ttattacttc
     1261 atctcgcagg aagagttcag gcaacgcatt gacaataaag aatttgcaga gtgggaggaa
     1321 gtgtatcccg gccagtttta cggcactttg cacagcgagt tggagcgact gtggagcgaa
     1381 ggcaaagaca tcattttcga tattgatgtg tatggagccg tgcgcctcaa aaagaaatac
     1441 ccggagcagg cgctcaccgt ttttgtgaag ccgccttcgc gggatgtact cgtcgaacgc
     1501 ctcaagggcc ggggtacgga aagcgaagaa accttccgga aacgcatcgt gaaagcggag
     1561 gaggaactgg cgtttgaaaa caaattcgat gtggttttag ttaacgatgt gctggaagcg
     1621 gcctttgccc gcgcagagga gattgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 ggaacaatag cggcgtgtgg ccgaaattat aaaaatactt cgcaatggac accctgatta
     1741 ccaacgatgt acgcatcagt gtagaggcgt tttaccaacc caactactcc aaacccgatg
     1801 tcggggagtt cgtctttgcc tatcgcatca ccatcgaaaa tctcaactcg tttaccgtgc
     1861 agctcctgcg ccgtcactgg catattttcg actctgccgg cgagcgccgc gaggtagaag
     1921 gcgagggcgt catcggcaag cagcccattt tgcagcccgg cggcgtgcat cagtatgtgt
     1981 cctggtcgaa tctcagttcc gaaatcggca gcatgcaggg tacttatcag ttccggcgca
     2041 ccgacaccga cgaaatgttc gacgtggaca ttccccaatt ccgactcatc actccttacc
     2101 gcctcaattg atccgccgtg ttgaaaattg acatgcacac ccatatcctg cccgaacggt
     2161 tgcccaattt tgcagaaaag ttcggctacg gagacttcat tcacctcgtt catcatcaac
     2221 ccggacgcgc ccaaatgatg aagggcgaca ccttcttccg cgaaattgag gccaactgct
     2281 gggaccccga actccgcatc cgcgaatacg cccaacaccg cacgcaggtg caggtagtgt
     2341 gtaccattcc ggtcatgttt tcctattggg ccgaacccga cgactgtctc gatctctcgc
     2401 gctttctcaa cgaccacatc gcccaactcg tacacgacta ccccaaaaac tacatcggcc
     2461 tcggcaccgt gcccatgcag gatgccgata ccgccatccg cgaattgcag cggctgaaag
     2521 acatgggtat tgtgggcatt cagattggtt ccaacatcaa cgaccgcaac ctgaacgaac
     2581 ccgaattctt ccccattttt caggcttgcc aagacctgag tctggccgtc atggtacacc
     2641 cctggaacat gatgggcgag gccgatatgc gccgctattg gttgccctgg ttggtaggaa
     2701 tgcccgccga aacctcccgc gccgcttgtt ccatgatctt cggcggcgtg ctggaacgcc
     2761 tgcccgaact gcgggtttgc ttctctcacg ccggcggctc gtttttgccc accatcgggc
     2821 gcatacaaca cggctacaac tgccgccccg acttggtggc cattgacaat cccaaaccgc
     2881 cgaccgacta cctcggcaag ttttgggtgg acagcatcac gcacgacccg gtcatgctgc
     2941 gctatgtcct cgacatggtg ggcgaagaca aagtgacgct gggttccgac tatcccttcc
     3001 ccctcggcga cctcgaaatc ggcgctttca tcgaagagat gggcctgccg gaaaccaccg
     3061 ttcaaaaaat attttgcgaa aattcgctaa attggctcgg tttgcaaaaa aatgcccaac
     3121 tttacagcgt ctgagcgagt aacgaacagg catttttatc caaagcaata ccatttatcc
     3181 catcatggaa gcatacattg taaaagccta tcgcagcgcc gtaggcaaag ccaaaaaagg
     3241 cggattccgc aactaccgct ccgacgacct cgccgtggac gtcgtcaaat acctcttcga
     3301 ccacacgccc gaactcgacc ccgctatggt ggacgacgtg atcgtgggtt gcgccaatcc
     3361 cgaaggtgaa caggggcttc agatcggacg ccagatttcg ctccgcgctc tgggcaaaga
     3421 agtacccggc atgacggtca accgctactg cgcttccggt ttggaaacca tttccattgc
     3481 cgtagccaaa atccgcgccg gcatgggcca tgtgtatgtg gccggcggca ccgaaaacat
     3541 gagtatgatt cccatgacgg gctacaaatt ggcgcccagc tatgccgtag ccgccaacac
     3601 gcccgactat ctggtaggca tgggcctcac tgccgaagcg gtggccaaca aatacggcat
     3661 cagccgccag gccgccgatg agttttcgta tcaatcgcac ctcaaagcgg cccgcgccat
     3721 tgaggaaggc cgtttcagcg agcaaattgt gccggtgaag gtcaaggagg tgtttgtaaa
     3781 agacaacaag cgcgtagaaa ccgagagcgt ggtggctatg gacgaaggcg ttcgccgcga
     3841 tacgacggtg gaagggctgg ctcaattgcg cccggctttt gccgccaacg gcatcatcac
     3901 agccggcaac gcctcccaaa cctccgacgg cgccgctttt gtgctggtgg tgagcgaaga
     3961 cattgtaaaa caatacaatc tgcaacccat cgcccgcatg gtttcctgcg ccgtggccgg
     4021 cgtggaggcg ctctacatgg gcatcggacc ggtggcggct attcccaaag cgctggctca
     4081 gggcggcctg aaactctccg atatccagca aatagaactc aacgaagcct ttgccgctca
     4141 ggcattggcc gtcatccagg aagccggcct tgatcccgag atcgtgaacg tcaacggcgg
     4201 ggccatcgca ttggggcatc cgctcggctg cacgggcgcc aagctctcca cgcagctctt
     4261 ccacgaaatg cgccgccggg gccagaaata cggcatggtc acggcctgcg taggcggcgg
     4321 acagggcatt gcgggcatct acgagttgct caactgaggg cgcggctgcg gcggtgattt
     4381 ccgttctcat tccggtttac aatttcgatg tgcggccttt ggtagaatct ttggccgggc
     4441 agtgccttgc gctggaatcg ccctgggaaa tcctttgtct ggacgacggc tctacggccg
     4501 aatttcgcgc attgaatcgc gctttgcagg ccattcccgg cgttcgctat gaggaactgc
     4561 cgcaaaacgt aggccgctcg gccattcgca accgcctggc cgaaatggct tcctttccat
     4621 atttgctgtt tatggactgc gattcggcgg tagtgcgccc cgactacatg gcgcagtacg
     4681 cggcccattg ccggcccgat gcggtgctgt gcggcggcag ggtatatgcg tccgatccgc
     4741 cggaaaacgc cgcttttttg ttgcattgga cggtgggcaa atcccgcgaa gaatggtcgg
     4801 cagcgcgccg gtcggtgcag ccctggcacg gttttatgac caataacttc ctcattcccc
     4861 aaaaaatact attgtccatc cgcttcgatg agcgactgcg cgagtacggc cacgaagaca
     4921 cgctgttcgg cctcgaattg gagcggcggc agattccggt actgcatttg gacaacccgc
     4981 tggagcatat cgggctggag ccggccgcag tgtttttgca aaaatcggag caggcggtac
     5041 aaaacctgcg ccgcttgcgc gaattgggca ttgttgtacc cacccgcttg gtgagcatca
     5101 gcgccgccgt cgagcgggcg ggcatgaggc ggcttgtgct gtttttgttg aggcgtctgg
     5161 aggcgcgctc ccgtcgaaac ctgctgtcgg agcgccctgt gccggcatat ttggatgcgc
     5221 tgaaactgag gtggatgctg gggaaatgag tgcctcggaa tatttttccc gccgatattg
     5281 ctttacccaa acaaagcccc tatctttgct gtcattcaag gttttatatg aatccctgat
     5341 ggaggaaaga agcatgaaaa agaaacaatt gaaaatagag gatttcagcc cttcgctgtt
     5401 ctgggacgtg gacaagacga cgctggactt tgagaaaaat gctattcatg tcgtagaaaa
     5461 ggctttgagt cgtggaagtt ggcaagattt taaaactgcc cttaattact acggccgggg
     5521 cagaatgaca gagttgattc aaaaaattcg ttatctggac gaaaggacta ctcaattcgt
     5581 tagcgtatat ttccaaattc ctttaactga attgcgatgc tacacctgga gtcggtcgaa
     5641 cccttcacat tgggattatt gaaatctttg atgtcccgaa aatatctgga tcaatttgtt
     5701 ttagtcggcg gcacggcatt ggcgctacaa atcgggcatc ggaaatctgt tgatctggat
     5761 atgtttacgg tgtcggacat agacacagac gctctgttgg aaaatttaca acaggactat
     5821 tccgttcagc ctaaagtgcg aacccaaggt agcctcatca gcgatataga aggcataaag
     5881 gtggatttca tccgattcaa gtatgccttt acatacccga tcgaggagat agatggtatt
     5941 cggctgttgt cagtaaaaga tattgctcct atgaaattgg atgccatcac tgggcgggga
     6001 agcaaaaaag acttttatga cctttatttt ctgttagaac ggttcacatt gccggaaatt
     6061 ctggaactat acctccaaaa gtatccacat tccacacttt ttcatgtaat taaaagcctc
     6121 acatggtttg aagatgcaga tccgcaggct cttcccgatg tgttggaaaa aaaaaatact
     6181 tgggcgcggg tgaaaaagcg tattgaaaaa gcagtagcaa aactttaatt taggagggtg
     6241 aaagttttca aattccttta cgagcaattg cgataaaaaa tacagccgga aaggcacatc
     6301 acgttatgat acgtgcggat agtggatttc ggtatcgggc agtgtcttaa tagaaatatt
     6361 tttcnnnnnn nnnnnnnnnn nncccgccga tattgcttta cccaaacaaa gcccctatct
     6421 ttgttgtcgt tcaaggtttt atatgaatcc ctgatggaga aaagacgagc gtctgaaaaa
     6481 gtcgttggag gtatgggtag ggtataaagc gggaatgagt gagattggtt gtagagggga
     6541 ttgcaggaaa tgggcgttaa tgccaaatat aataaaaaaa tagaaaatga ataacctaat
     6601 aaaaatcgtc atattaaacc tgatttcagt ttcactttcg gggcaaatta tatctgattt
     6661 caacctacct aaaacaatat catcgactgt acaagatacg atattaattg atccgatttc
     6721 ttgtattcct ctttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga agggatgaaa
     6781 ttgtagaaag cgcaacggga tttattttgg aggataattc taaattttat ctaataacta
     6841 actggcatgt catagcaaat agaaacccta ccgatagcac cgcaaataaa ataaaagtca
     6901 aaaatgcagt tggaaatgaa ttagaaattg atgtttctga ccctgaaaaa ttacttattt
     6961 attttcatgg caagacactt ggagaatgga taccgatcac aatcgacctt tacgataaag
     7021 gaagaaaaaa gtggttagaa catccttttg ggaaaaaagt tgatgtagtt gcactgccaa
     7081 taaatctgac tgatgaacaa aagaataaga ttcacttata ctcactaaaa tttgatgaat
     7141 acaacgatga aatagtaaaa tatccatcta taaatctcag cattattgga ttccctttcg
     7201 gtttttccag ttatggacga tttcctattt ggaaaacagg tcaattgtcg tcagactttg
     7261 atttaaactt tgacgacctt cccatttttt tgattgatgc ttccacaaga agtggtatgt
     7321 caggttctcc tgttattttg aggcaagtgg gcgcgattta tacaaaacaa gtggttggga
     7381 tgggaggtta tgtacaagag ttcttaggaa tttattctgg aagaataggc aatgactctg
     7441 atattggaat agtttggaaa atagagtgtt tgaaagaaat aataaaataa caatcgccac
     7501 tacctgccgc cccgcaccct cccctcctta cacaaatttc tcattatcag ctaaataatt
     7561 ttaaaaaaca cccatcatcg cctaccaccc tccggcctct tttcccacat ttttttacta
     7621 cccagcttgc cgggagtttg tatttcgcct cccgaatcag ggttggagta gcggcggatg
     7681 cgggggctgt tggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnngcac caaattgaac atttcaatct ttcaaaacgg cttgctcctc
     7801 ttggctttaa gcattttttt gctttcatcc tgcggcaata aaagccaatc cgccgacgca
     7861 gcagcacagc cggaggtaag aatcagcgaa acgtcggcca ttcttaccaa atttaacttt
     7921 gagcatttcg gcagctacga cctttctacc tggaagctga tgccaaaggt tcaccagggt
     7981 gatgttgtgc ttgaggttac gacttacaca aaggacggca gtaaaatgga tgttaaagaa
     8041 tcggacatgg gagaaggnnn nnnnnnnnnn nnnnnnnnnn nnnnncctac gagcttactg
     8101 gtcccgacgg caaggtcttg aagacgcgtg atttgtcatt aacaaccgaa tcggatgctg
     8161 aatcggaaaa actgcctgtt accataacag aaaccgtcag ggactacact gtaaatcctc
     8221 caaaacagtt tgaaaggata caaaaaaccg gcaagcttca ccgtgaactg aaaccgttgc
     8281 cgacggcggc aaccggagct tgggtggaaa gcactgctga ggcatatttt taccccgagg
     8341 ataataatta atcgccggca caccgtcgct cctcctgcgt cgtcactacc ctcggccccc
     8401 tcacgcaacc gggcagcacc tattcgcgcg ccgaagaaga acgattattg acagtgggcc
     8461 ggggcgctat gggcacagac ggcgcgggca gggcgcgttt gcaaacgggc gtgaacaagt
     8521 cgtccaaaac catcgggcgg cagggcttga tatcgtccca aataaaaccc tccaaacgca
     8581 gcgtctgatg atcggcctgt tgcatggggg ccattttcga tttgggttcg gctaagaatt
     8641 tgatgcgttt gatctggttg ttgccgaaat gcagcaccat gtcgctgcac tccgttttat
     8701 tggccccgat gtaggctccg gcatcgtcgc gggcataata aatagactgc gcgttgcccg
     8761 atacatccat cacgcggagt tccttttcct caaaacgggc ggtgatattg cgccctttga
     8821 tctgattgaa aaaacgctca tcgggcgagt tgataatcag tccgttgccg aacaggaaga
     8881 tgcggttgat cttattgtcc acgagttcca agtgcatggt atctgcgctg aactgcgtcg
     8941 tggtatccga ccaaataaaa ggcgctttgt agagcctgaa aatgctgtcg acgctactgt
     9001 aactgagtga atcgcaaatg acctggaggt cgtttttgag gatgcgcaca tcattgtagg
     9061 caaacagggc gcggctgctg tcgccttgca gcgtgtccaa gcgcagcgag agcagggtgt
     9121 cggcggtcat gtagagggtg tcgccttcga ggagagagcg gagcaggggg cgattggctc
     9181 tggcgccgga tgctttgatg aagccggttt cgcggttgta ggcggcgcgt tcgcaaacga
     9241 tgttcaggtt ggcgctggtg tcttgccacg acacgttgcc cgtagcgatg cccaaacctt
     9301 gctcctcatc gaaatccacc tcatcggctt ccagaatctg cggcggatcg gcaatgcggc
     9361 tgcgcccgcg ggtggcgtag gttttctttt tgccgtcgta gatgatttct tcggagcgga
     9421 tgtcctgatc ggcatccacg agatgggcct tgcctttgag gaacgttttg tcggcttttt
     9481 gatcgtattg aatgaggtcg gcggtagcgc ggcgggcgcc ttcttcaaac caggcattgc
     9541 tttgcagggt ataaacgtcg aatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcggc
     9601 tttgacaaac tgcgcgtttt ggttgaattc ggcgaagttg gtttgcgtat tgtaaaaacc
     9661 gctttcggta taaacctttg attcgccggt agctatgatg gtggggccga ggaaaaaggc
     9721 cgttttggtc tccacgttga atttgagcgt gtcggacttg agtgtaaaat cggggtcgtt
     9781 gacctctact ttttctttga aatagacctc tttttgttgc acaaagtagg ttcctcgccg
     9841 gcttttgagt ttgcttttgc cattggtgag cagggcgcca tccacataag tggccacttt
     9901 ggtattgagg tcgtagtgga gtttatcggt gaataatttc tgttcgccat tgaccaacac
     9961 cacgtttccg aagagttccg cttgccgcaa ctcgctgaga tacaccaaag aatcggcaaa
    10021 aataactgcc gtatcgccct gctgaatgct gactttgccg acagcaatga cgcgcaccct
    10081 gtcttcgatg gtggccgaat cgcaatacat atatacgctg tcctgatgga gttgcacctc
    10141 tccaacaagc ttctgcaaga ccttgccgcg ctgctgaagg aggctccact gacgcgcaaa
    10201 gtcaatgtcc actggtttgt tgcttttctt ttcctgtccg aaaagcatgg cgggcatcac
    10261 aacagcccag atgaaaatga ataaaaagcg atacacgatg cgttgtctgg ttttgataat
    10321 caattgtttc gggttccggg ttcaaggttc cgggttcaag gtttcgggtt gctcacccct
    10381 cacccactca cccactcacc cctgaaaatt gcaaacgacg gaggcctgcc tcatgtttct
    10441 tgcgcaaagg aaaacaatat tttgtcggct aactggttta atttatggcg attatgaacg
    10501 aaacagctcc aatgagcgtt gcgggaagta ggtcggattc tcgataaagc aagtgaagcg
    10561 gcattgaaaa gtgagttttt ccaaataaaa tttgaaaaaa tcttcccaat gtcgttaatg
    10621 tttcgttctt ttgcactcgg aaagtcattc ttcacaaatt ctcattcaaa atgtctgaca
    10681 aattagataa aattgacctg aaaatccttc gcattttgca ggaaaacagc aagatcacca
    10741 atcttgacct gtccaaaaaa atcggactct ctcctgcacc cacgctcgaa cgcgtgaaaa
    10801 aattggagca aaccgacgtg atcgaaagct atcacgccaa ggtgaccccc caatctatcg
    10861 gcctcaatgt gaagaccttt gtgctggttt ccttggcttg gaaaaaggaa aacgcactga
    10921 accagttcct ggaaaaaatc atggaaattg atgaagtgac cgagtgttac atcattaccg
    10981 gcgaagccga cttcctgatg aaagtggtgt gcaaagacat tcccacttac gagcaactgc
    11041 tgttcaaaac actgtctcaa attgacgaaa tcgagcgcct ccgcacgctg atgacgctct
    11101 ctaccgtcaa agattccaag attttgcctt ataagtacga ataatcggct gtaacttcgt
    11161 ttcggcatta ttcgcaaaaa accgcttccc gactttcgga gagcggtttt ttatttactt
    11221 cgcctttatg aaaacaactg ctctgctctg ggaatgccga cacgccgaag cgcccggcac
    11281 atcgtttttg ttcggctcca tgcacgttcg cgacagccgg gcattcgccg gtttgccgcc
    11341 cgtttatgtc gctatggagc agtgcgaggc gttcgcagcg gagttcaacc tcgatgaggc
    11401 gcc
//
LOCUS       DAORLF010000099        11402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3039_length_11402_cov_1.811756, whole genome shotgun sequence.
ACCESSION   DAORLF010000099 DAORLF010000000
VERSION     DAORLF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_11402_cov_1.811756"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>936
                     /locus_tag="PK198_09900"
     CDS             <1..>936
                     /locus_tag="PK198_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF39091.1"
                     /translation="PVSKSEWTARMEKELKGKPLADLNWHLDLDDHIITAPFYHPEDQ
                     AGAPQPILRNNPDNDWEIGEYIEVQQMDKANQEALEGLQGGVQAPLFQLGRSISDQDM
                     AQLLQGIDVSMISTHFGQYFPGKDPQRLLFQFHRAAQKQGAQVAELKGSVDCDPILDW
                     PEPPFDMQADMIRFTADVMPLFKVIQVNARRLHSGPENTAWELAYTIAKGSEYLAQMG
                     LRGLDAALVNRHLQFSLTVSASYFVEIAKLRALRILWSNILASYGANADAGCTVEVHL
                     APETQEKDPNTNLIKAATQVMSAAIGGADIIYVLP"
     assembly_gap    937..989
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1188..2885
                     /locus_tag="PK198_09905"
     CDS             1188..2885
                     /locus_tag="PK198_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF39092.1"
                     /translation="METYRNTDLNNLLSYFYQWESEKPDAVFMRQPYGNTWKTITWAE
                     AGAEARRIAAALAGIGMQAGDHIGIVSKNCYHWVIADLAIMMGGYVSVPFYPTLTAPQ
                     LEQVLQLSHCKALFVGKLDNWPDMREGVPAGIEIIRFPQYEGNSVVEEGWPWEGLLAA
                     YEPIAGNPERSLDELWSILYTSGTTGVPKGVMLDFFAPASLMENERRYDSLKLFSGTE
                     HRFFSYLPLCHIAERLIVEGAALLTGGEISFAESIESFAKNLQATRPTLFLGVPRIWT
                     KFQLAILERMPQKKLDLLLRIPIVNTLVRKKIRAGLGLDKARITVTGAAPMPDAVKNW
                     YARLGIHLQEVYGMTENCGGATLSPHDAIKPGTVGKPLPNARVGIVPETGEVTIQLPW
                     MMRGYYNEPEKTAETLRDGVLYTGDQGELDEEGYLKITGRISDTFKSAKGKFIVPGPI
                     EWSFAKNNYVEQVCVAGLGIPQPLALVVLSDIGKEAEVEKLRQSLQETLDHINSRLPN
                     YEQLKTVVVFQQPWSVENGLLTPTLKIKRHELNRRYQHLFAQWYEHNERLVLVRTEE"
     gene            complement(2995..>3939)
                     /gene="argE"
                     /locus_tag="PK198_09910"
     CDS             complement(2995..>3939)
                     /gene="argE"
                     /locus_tag="PK198_09910"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRF39093.1"
                     /translation="TDVVPVKGQAWDTDPFALTLKGDKLYGRGSCDMKGFLACCLASI
                     ELFQQADLKRPVYFAFSYDEEIGCLAAPALAAAINAHYTEKPKYAIIGEPSRMQPIVG
                     QKGICVLETTVHGSAGHSSRVKQEVSAIHVAARLITWLEQKMDALIAAGHTDDRFHPN
                     HSSLHSGIIGGGIAPNVIADKCSFHWDVRSIPADRIEDIIQDFEAYSRTLETALQQRF
                     AGARIETVVHHPPVPSLDTPEDSSVVPLIRRLSGVEQLATVAYAAEAGQFAEVGFEAV
                     ICGPGDIAQAHRANEFIEVAQLELGLEMMERLARELAE"
     assembly_gap    3940..3951
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3952..4170)
                     /locus_tag="PK198_09915"
     CDS             complement(<3952..4170)
                     /locus_tag="PK198_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRF39094.1"
                     /translation="MTRIETAVQILTDLVAFPVLGGESNLSIAAYICNLLEKNGIEYH
                     LVKNEAGDKTSIHCRIGPETDGGVILSGH"
     gene            complement(4205..>5191)
                     /locus_tag="PK198_09920"
     CDS             complement(4205..>5191)
                     /locus_tag="PK198_09920"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRF39095.1"
                     /translation="DMASMNRLRESPKLALQARWSLAAAYAAAGKQEIAKEVMKNLSR
                     KIEPYTELSYTYGSDLRDRAIILETMLQTGNKTGAAELVKYISEQLSSGRWYGTQTVS
                     WALMSVGKYVGQTGASNELKFSYQLGSQALVNAGSTSPLMQVKVPVDGAKKEVSVQNT
                     SKGVLFARLILRGQPAVGESQAAANDLRIAVQFKQMNGSPININNLAQGTDFYAEVTV
                     THPGNRPIPYKELALAQIFPSGWEIVNSRLDGIDGVASPSRADYIDIRDDRVNTFFDL
                     RERETKVYRVQLNAAYQGRYYLPAMLCEAMYDQSISATQPGQWVSVVGRDAI"
     assembly_gap    5192..5331
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5332..>5650)
                     /locus_tag="PK198_09925"
     CDS             complement(<5332..>5650)
                     /locus_tag="PK198_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39096.1"
                     /translation="LGERLDYLLQYPYGCLEQTLSGGFPQLYVGRLIELNENQKKTVP
                     NNIKATIERLKKFQTSNGGFAYWPGEQQPDQWSTSYAGHFLLEAKALGYAVPDNMLDN
                     WLKF"
     assembly_gap    5651..5862
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5863..>7733)
                     /locus_tag="PK198_09930"
     CDS             complement(<5863..>7733)
                     /locus_tag="PK198_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRF39097.1"
                     /translation="TGNWIAKVKVGGAVFDRVIKVETVKPNRLKINLETGKETLAAQD
                     NPLNVKLNSTWLHGAPAKSLKAVVEIQIKSAKTSFPKHGEFVFDDPGRRMETEPRTVF
                     EGNLSEDGQASFEARLLDGVTASGQMSVNFKTRVFEKGGDFSTDNKGFVYMPYDNLAG
                     VRIVPNKYKEKRFELKKAGKIQLLAVDSKGNPQPGRKLSAAIYKSSNDWWWDVDDSNN
                     ARFTNADNMQVELRSDFTTGNRGEAEWSVTMERWGRYFVRICDSESGHCTGDYFYAGY
                     SWDEDNGQGKREAAAMLTISADKPKYNTGDNVQLTIPAGEAGKALISIENGTRVLQSF
                     WVDTKAGDNKISFKATPEMAPTVYANISLLQPHAQTKNDLPIRMYGVVPISVEDPATH
                     LQPKIKMPDELKPESTVAVEVSEEKGRPMAYTIAVVDDGLLDLTRFKTPAPWESFYAR
                     EALGVTTWDVYDMVLGAYGGQLGRILSIGGDAALVPGANANAMRFKPVVVHLGPFYLK
                     KGEKKSHNIQIPNYVGSVRTMVVAADNGAYGHVEKTTPVKKPLMVLATVPRVLSPGET
                     LKVPVNVFAMDKKVRDVSVSISEKSGIVYAVNGSKQSLSFSKPGDQLVEFEVKVKEMT
                     "
     assembly_gap    7734..7743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7744..>9537)
                     /locus_tag="PK198_09935"
     CDS             complement(<7744..>9537)
                     /locus_tag="PK198_09935"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRF39098.1"
                     /translation="PEKTLPAQTPFVATVHLNKIFNGLPAEAETFQFDFRTRDQYFTV
                     EADGISAPDPDNLAQQEISGVVNTADVATEEEVQQLVTAKQEGKTLPVSWTQSGDQLT
                     HYFTVGKVNRDGKSSSVNLAWNGKPLDVDIKASKKVEVPALGNFKVVNVRVVQDQEQY
                     VLVHFSDPVSESQNLDGLIRLQNFDGTLRYVVNGNQVRVYPSRRLSGAYSLLVTTGVK
                     NAAQKGLSNQANWSVKFENPEPEVRLVGNGVIVPNSEGLMFPFEAISLRAVEVEVFKI
                     FNNNILQFLQTSTLDGNSYDLYRVGRIVVRKKIPLSDLASGMRTHEWARYSFDLGPLI
                     GKDPKAIYQVRIGFRPEDAAYGCEGQAKQDEEAALSVVNTFNEEEEQDENSGMMSYWY
                     GPDGWYEGYSWDDRNDPCKPAYYNSDRFVQRNVLASNVGLIAKAGKDNSVAVTVSDLR
                     NAGPVSGAKVDFYDFQQQLLTSVQTDAKGGATAQLPRKAFVAVATWQDQRGYLRMDDG
                     NSLSLSRFDVGGAEPQKGLKGFLYAERGVWRPGDSIYLNFLLEDRTAKLPADYPITLE
                     LYDPRGQLYHKRNTSNNTNRLYPLYFKTGSDA"
     assembly_gap    9538..9550
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9551..9867)
                     /locus_tag="PK198_09940"
     CDS             complement(<9551..9867)
                     /locus_tag="PK198_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39099.1"
                     /translation="MRKPFISMSVLLLSLAVFAVISSTVSCTKKPDNISRVIPESIGA
                     YVYAFTSGMISKASHIKVQFATAAVEAASVGKEAAAGLISFSPGIKGKAVWEDERTLV
                     FQP"
     gene            10001..10630
                     /locus_tag="PK198_09945"
     CDS             10001..10630
                     /locus_tag="PK198_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRF39100.1"
                     /translation="MLPLFPLQIVVYPGEKLNLHIFEPRYKQLMQACENENITFGVVP
                     FLDGRLTSFGTEVVLTEVQKRYDNGELDVRTQGIGLFHLDEFFKTAPGKLYPGGDVTR
                     VDIVYDGTRKLGGHLLSMARSLFSMLSIEKELPADPDRFLAYDIGHYVGLSQQQEYEL
                     LCIAEENERREYLIEHLEQLLPLVRETEELKRKVRMNGHFRNLTPPEFL"
     assembly_gap    10729..10788
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11000..11162
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11163..>11402
                     /locus_tag="PK198_09950"
     CDS             <11163..>11402
                     /locus_tag="PK198_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
                     /protein_id="HRF39101.1"
                     /translation="LTSKGDVSFTPVSAGIDLSFIGPMAPNCPLTADPVCGNNGVTYL
                     NACFAQAAGITQYASGVCFSTCINPAAIQLNAVCT"
BASE COUNT         2409 a         2953 c         2856 g         2521 t
ORIGIN      
        1 ctcccgtttc caaatcggaa tggacggcac gcatggaaaa agaactgaaa ggcaagcctt
       61 tagcagattt aaattggcat ctcgacttgg atgaccacat cattacagcg cctttttatc
      121 acccggaaga ccaggccggc gcgccgcagc ccattctcag aaacaacccc gacaacgact
      181 gggaaatcgg cgagtacatc gaagtccagc aaatggacaa agccaaccag gaagcgctcg
      241 aaggcctgca gggcggcgtg caggcgcctt tgttccaatt ggggcgctcc atcagcgatc
      301 aggatatggc gcagctcttg cagggcatag acgtttccat gatttccacc cattttgggc
      361 agtacttccc cggcaaagac cctcagcggc tcttgtttca gtttcacagg gcggcacaaa
      421 aacagggcgc gcaggtagcc gaactcaaag gctctgttga ttgcgacccc atcttagact
      481 ggcccgaacc gcctttcgat atgcaggccg acatgattcg ctttacagca gatgtgatgc
      541 cgctgttcaa agtcattcag gtgaacgccc gccgcctcca ttccggcccc gaaaatacgg
      601 cctgggaact ggcgtacacc atcgccaaag gcagcgagta tctggcgcag atggggttgc
      661 gcggtctgga tgccgctttg gtcaatcgtc atttgcagtt ttcgcttacg gtcagcgcca
      721 gctattttgt agaaattgcc aaactgcgcg ccctccgcat cctttggagc aatatcctcg
      781 cctcctatgg cgccaatgcc gatgccggct gcacggtcga ggtacacctg gcgccggaaa
      841 cacaggaaaa agaccccaac accaatctca tcaaagccgc cacgcaggtc atgtcggcgg
      901 ccatcggcgg agcagacatc atctatgtgc tgccctnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcgcatggcc cgcaatgtgc aacacattct
     1021 caaaatggaa agccacctag atcgcgtgca ggacccggcc gccggcagct attacatcga
     1081 atcgctgacc gatatgttgg tacaacgcgc ctgggctttg tttcaggatg tggaggccaa
     1141 aggcgggtat ttgatggcgg gatgatttga ttaaaaacac ttatatcatg gaaacataca
     1201 gaaacaccga tttgaacaac ctactttctt acttctacca atgggaaagc gaaaaacctg
     1261 atgccgtgtt tatgcggcag ccctacggca acacttggaa aaccatcacc tgggccgaag
     1321 ccggcgccga ggcgcgacgc atagcggcag cactggcggg catcgggatg caagccggcg
     1381 accacatcgg catcgtgtcg aaaaattgct atcactgggt catcgccgat ctggccatta
     1441 tgatgggagg ctatgtttca gtgcccttct accctaccct cacggcgccg caattggaac
     1501 aggtactcca actcagccat tgcaaagcgc tcttcgtggg caaactagac aactggcccg
     1561 acatgcgcga gggcgtaccg gcaggcatcg agatcattcg ctttccgcaa tacgagggca
     1621 acagcgtagt ggaagaaggc tggccctggg aaggcttgct cgctgcttac gaacccattg
     1681 ccggcaatcc cgaacgctcc ttggacgaac tctggagcat cctttacacc tccggcacca
     1741 ccggcgtacc caagggcgtc atgctcgact tttttgcccc agcctccctg atggaaaatg
     1801 agcgccgcta cgacagtctc aaactctttt cgggtaccga gcatcgcttt ttttcttacc
     1861 tgccgctctg ccacatcgct gagcgactta ttgtggaggg cgccgctttg ctcaccggcg
     1921 gcgagatttc ctttgcggaa tctatcgaat cctttgccaa aaacttacag gccacccgcc
     1981 ctaccctctt cctcggcgtg ccgcgcatct ggaccaaatt ccaattggcc attctggaac
     2041 gcatgccgca gaaaaaactc gacttgctgc tgcgaattcc catcgtcaac acactcgttc
     2101 gcaagaagat acgcgcagga ttaggtcttg ataaagcccg catcacggtc accggcgccg
     2161 cccccatgcc cgacgccgtc aaaaactggt acgcccggct cggtatccat ttacaggaag
     2221 tatatggcat gactgaaaac tgcggcgggg ccacgctttc gccccacgac gccatcaaac
     2281 ccggcaccgt gggcaaaccg ctgcccaatg cccgcgtcgg tattgtgccg gaaaccggcg
     2341 aagtgaccat ccaactgccc tggatgatgc gcggctacta caacgaacct gaaaaaactg
     2401 ccgaaaccct gcgcgacggc gtgctctaca ccggcgatca gggcgaattg gacgaggaag
     2461 gctatctcaa aatcacgggt agaatttcgg atacatttaa gtcggccaaa ggcaagttca
     2521 tcgtgcccgg ccccatcgag tggagttttg ccaaaaacaa ctatgtggag caggtttgcg
     2581 tagcgggtct cggtattccg cagccgctgg ccttggtggt gctgtccgac atcggtaagg
     2641 aggcagaagt ggaaaaacta cgccaaagct tgcaggaaac attagatcac atcaacagcc
     2701 ggctgcccaa ctacgagcaa ctcaaaacag tagtcgtttt tcaacagccc tggagcgtgg
     2761 aaaacggcct gctgacgccg acgctcaaaa tcaaacgcca cgaactgaac cggcgctacc
     2821 aacacttatt tgctcaatgg tacgagcaca acgaacgctt ggtgctggtg cggacggaag
     2881 agtgacgacg acgatgcgcg aggtgcagac cgctcgcgcg agattctacc gctcgcgcga
     2941 ggttctacct cgtgcggact atctgcgggg ttcaaacccc gcttacgatt ctttctattc
     3001 tgccaactcc cttgccagcc gctccatcat ctccagccca agctccaact gcgccacctc
     3061 aataaattca ttggcgcggt gtgcctgtgc tatatcgccc gggccgcaaa tgaccgcctc
     3121 gaagcccact tccgcaaact gtcccgcctc ggccgcatac gccaccgtag ccaactgctc
     3181 caccccgctc agcctgcgaa tgagcggcac cacgctgctg tcttcgggcg tgtctaatga
     3241 cggcaccggc gggtgatgca ccaccgtctc aatgcgggcg cctgcgaaac gctgctgcaa
     3301 tgctgtttcc aatgtccggc tgtatgcctc aaaatcctga atgatgtcct caatgcgatc
     3361 tgccggaata ctgcgcacat cccaatgaaa gctgcacttg tcggcaatga cattgggagc
     3421 tatgccgccg ccgatgatgc cggagtgcaa agaactgtga ttggggtgaa accggtcgtc
     3481 ggtatggccg gcagcaatca gcgcgtccat tttttgctcc aaccaggtaa tcaagcgggc
     3541 cgcaacgtgg atggcgctca cctcctgctt cacgcggctg ctgtgccccg ccgacccgtg
     3601 taccgttgtt tccaatacgc agataccttt ttgacctaca atgggttgca tgcgcgaagg
     3661 ctctccgatg atggcatatt tcggcttctc ggtataatgc gcattgatgg cagccgccag
     3721 cgcaggcgcc gccagacaac cgatttcctc gtcgtaagaa aaggcaaaat acaccgggcg
     3781 tttcaggtct gcctgctgaa ataactcaat ggaagcgagg caacaagcca aaaaaccctt
     3841 catatcgcaa gagccgcgcc cgtagagttt atcgcccttc agcgtcagcg caaaaggatc
     3901 ggtatcccag gcctgccctt tcaccggcac cacatccgtn nnnnnnnnnn natggccgga
     3961 gagaatgacg ccgccatccg tttccggccc gatgcgacag tgaatggagg ttttgtctcc
     4021 ggcttcattt tttaccaaat ggtactctat gccgttcttt tccaacaaat tgcaaatgta
     4081 tgcagcaatg gaaaggttac tctcgcctcc caaaacggga aaagcgacga gatcggtcag
     4141 gatttgaacg gctgtttcta ttcgcgtcat ttttgaaata atttttgcct caatactatc
     4201 tcgctcaaat cgcatcccgt cctaccaccg agacccactg tccgggctga gtagccgaaa
     4261 tagattgatc gtacatggcc tcgcaaagca tagccggcag gtagtaacgg ccctgataag
     4321 ccgcgttgag ctgcacccga tacactttgg tttcccgctc acggagatcg aagaacgtgt
     4381 tcacccggtc gtcgcggatg tcaatgtagt cggcccggct cggcgacgct actccatcaa
     4441 tgccgtccaa acgggaattg acgatctccc agccggaggg gaagatttgc gccagcgcca
     4501 gttctttgta cggaatgggc cggttgccgg ggtgcgtcac cgtcacctct gcataaaaat
     4561 cagtgccttg ggccagattg ttgatgttga tcggcgagcc gttcatttgt ttgaattgaa
     4621 ccgcaattct aaggtcgttg gcggcggcct gcgattcgcc cactgccggc tgaccgcgca
     4681 ggatgaggcg cgcaaagagg acgcctttgc tcgtattttg caccgacact tcttttttgg
     4741 cgccatccac cggcactttt acctgcatga gcggcgaggt agaaccggcg tttaccaatg
     4801 cctgactgcc caattgatag ctgaatttca gttcattgga cgcgccagtt tgtcccacat
     4861 atttgcccac cgacatcaaa gcccaggaga cggtttgggt gccgtaccag cgcccgctgc
     4921 tcaattgttc agagatgtat ttcaccaatt ccgccgcgcc ggttttgttg ccggtttgca
     4981 gcatggtttc caaaataatg gcccggtcgc ggaggtcgga accgtaagtg taagacaatt
     5041 cggtgtaagg ctcgattttg cgactgaggt ttttcatcac ctctttggca atttcctgct
     5101 tgccggcagc ggcataagcg gcagccagac tccaacgggc ttgcaaggct aatttgggcg
     5161 attcgcgcag gcggttcatg gaggccatgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaatttga
     5341 gccagttgtc gagcatattg tcgggtacgg catagcccag cgctttggct tccagcagga
     5401 agtgccctgc gtaggaagtg gaccactgat cgggctgctg ctcgccgggc cagtaggcaa
     5461 aaccgccgtt ggaagtctgg aatttcttca ggcgctcaat ggtggctttg atgttgttcg
     5521 gcactgtttt cttctgattt tcattgagtt ctatcaaacg cccgacgtac aattgcggga
     5581 aaccgcccga cagagtttgc tccaaacagc cataaggata ttgcagcagg tagtccaaac
     5641 gctcgcccaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcatttc ttttactttg
     5881 acttcaaact cgaccaactg atcgccgggt ttgctgaaag acaggctttg tttggagccg
     5941 tttaccgcat agacaatgcc gctcttttcg ctgatggaca cgctgacgtc gcgtactttt
     6001 ttgtccatag caaacacatt gaccggcact ttgagggttt cacccggact gagcacgcgc
     6061 ggcacggtag ccaaaaccat gagcggtttt ttcaccggcg tggttttttc cacatggcca
     6121 taagccccgt tgtcggcagc taccaccatc gtgcgcacag agcctacata gttgggaatc
     6181 tgtatgttgt gcgatttttt ttcacctttt ttcaaataaa acggccccag atgcaccacc
     6241 accggtttga aacgcatggc attcgcattg gcgcccggca ccaaagccgc gtcgccgccg
     6301 atgctgagaa tgcgcccgag ttggcctcca tacgccccca acaccatatc atatacatcc
     6361 caggtggtga cgcccagcgc ctcgcgggca tagaatgatt cccagggcgc cggcgttttg
     6421 aaccgggtga gatcgagcag gccgtcgtcc actacggcaa tggtgtaagc catcgggcgg
     6481 cctttttctt ccgatacttc taccgctacg gtgctttccg gtttcagttc atcgggcatt
     6541 tttattttcg gctggaggtg cgtggccggg tcttccaccg aaataggcac cacgccgtac
     6601 atgcgtatag gcaggtcgtt tttggtttga gcgtgcggtt gcagcagcga gatgttggca
     6661 tacacggtag gcgccatctc gggagtagcc ttgaagctga ttttattgtc gccggccttg
     6721 gtatccaccc agaaggattg cagcacgcgg gttccgtttt ctatggaaat cagcgcttta
     6781 cctgcctcgc cggcgggaat ggtgagctgc acgttgtcgc cggtgttgta tttgggtttg
     6841 tcggcagaga tggtcagcat ggcggcggct tcccgtttgc cctgcccgtt atcttcatcc
     6901 caggagtagc ccgcgtagaa ataatcgccg gtgcaatggc cgctttccga gtcgcagatg
     6961 cgcacaaaat aacgccccca gcgctccatc gtcacgctcc attccgcttc cccccgattg
     7021 ccggtagtga aatccgagcg cagttcaacc tgcatgttgt cggcgttggt gaaacgcgca
     7081 ttgttggagt cgtccacatc ccaccaccaa tcgttggagc ttttgtaaat ggctgccgag
     7141 agtttgcgcc cgggttgcgg gttgcctttt gagtccacgg cgagcagttg gattttgccg
     7201 gcttttttga gttcaaaacg cttctcctta tatttattag gcacaatgcg caccccggcc
     7261 agattgtcgt aaggcatata aacaaagccc ttgttgtcgg tgctgaagtc gccgcctttt
     7321 tcaaaaaccc tggttttgaa attgaccgac atctggcccg aagccgtaac gccgtctaat
     7381 aatctggcct caaaacttgc ctgtccgtct tcgctgaggt tgccttcaaa taccgtgcgc
     7441 ggctcggttt ccatgcggcg accgggatcg tcgaaaacaa attcgccgtg tttggggaaa
     7501 ctcgttttgg ccgatttgat ctgaatctct accacagctt tgaggctttt ggccggagcg
     7561 ccgtgcaacc aggtggaatt gagttttacg ttcagcggat tgtcttgtgc cgcgagcgtt
     7621 tcttttccgg tttccaagtt gattttcagt cggttgggct tcaccgtttc caccttgata
     7681 acgcgatcaa agacagcgcc gcctactttc acttttgcta tccaattgcc ggtnnnnnnn
     7741 nnncgcgtcg gagccggttt tgaaatacag cgggtagagg cggttggtgt tgttggaggt
     7801 attgcgtttg tggtacagtt gcccgcgagg atcatagagt tctaaggtga tgggatagtc
     7861 ggcaggcagt ttggccgtgc ggtcttcgag caggaagttg agataaatgg aatcgccggg
     7921 ccgccatacg ccgcgctcgg catacaagaa gcctttcagc cccttttgag gctcggcgcc
     7981 gcccacatcg aaacggctga gggagagcga attgccgtcg tccatgcgca ggtagccccg
     8041 ctgatcttgc caggtagcta ccgccacgaa ggctttgcga ggcaattgcg ccgtagcgcc
     8101 gcctttggca tctgtttgca ccgaagtgag gagttgctgt tggaaatcgt aaaaatccac
     8161 tttggcgccc gaaaccggcc cggcattgcg caggtcggaa acggtgacgg ccaccgagtt
     8221 gtctttgccc gctttggcaa taagccccac attggaagcc agcacattgc gctgcacgaa
     8281 gcggtcggag ttgtaataag ccggtttgca ggggtcgttg cggtcatccc aggagtagcc
     8341 ctcgtaccag ccgtcggggc cgtaccaata gctcatcatg ccggaatttt cgtcctgttc
     8401 ttcctcttcg ttgaacgtat taacgaccga cagagcagcc tcttcatcct gtttggcctg
     8461 tccttcgcaa ccatacgcgg catcttcggg ccggaagccg atgcgcacct gatagatggc
     8521 cttcgggtct ttgccgatga gcggccccag atcgaaagag tagcgcgccc attcgtgcgt
     8581 gcgcatgccg gaagccagat cgctgagggg tatttttttg cgcaccacaa tgcgccccac
     8641 gcggtacagg tcatagctgt tgccgtctaa ggtgctggtt tgcaggaatt gtaaaatgtt
     8701 gttgttgaat attttaaaaa cttccacctc tacggcgcgc aggctgatgg cttcaaaggg
     8761 gaacatcagc ccttcggaat tgggcacgat gacgccgttg cctaccaagc gcacttcggg
     8821 ttcggggttt tcaaatttga ccgaccagtt ggcctggttg gaaagccctt tctgcgccgc
     8881 gtttttcacg ccggtagtca ctaaaagcga ataagcgccg gaaagcctgc gagatggata
     8941 aacgcgcacc tgattgccgt tgacgacata acgaagcgtg ccatcaaaat tttgaagccg
     9001 gatgagacca tcgagattct ggctttcgga aacgggatcg gagaaatgca ccaacacata
     9061 ctgctcctga tcctgcacaa cgcgaacatt gaccactttg aaattgccca atgccggcac
     9121 ctccactttt ttggaggctt tgatgtccac gtccaaaggt ttgccgttcc acgcgaggtt
     9181 gacagaggat gatttcccgt cgcggttgac ttttccaacg gtaaagtaat gagtcagttg
     9241 gtcgccgctt tgcgtccagg aaacaggtag cgtcttgccc tcctgtttgg cggtgacgag
     9301 ctgttgaacc tcctcttcgg tagccacatc ggcggtgttc acgacgcccg aaatctcttg
     9361 ttgagcaaga ttgtcggggt cgggagcgga aatgccgtct gcttctacag taaagtactg
     9421 atcgcgggta cggaagtcga attgaaaagt ttctgcctcg gcgggcaatc cgttaaaaat
     9481 cttgttgagg tgaacggtgg ccacaaaagg cgtttgcgcc ggcaatgtct tttccggnnn
     9541 nnnnnnnnnn tccggctgaa acaccagtgt gcgctcgtct tcccaaacgg ctttgccttt
     9601 gatacctggc gaaaaactga tgagaccggc ggcggcctct ttgcctaccg aggcagcctc
     9661 gacagcggca gtggcaaatt gtaccttgat gtgcgaagcc ttggaaatca tacccgacgt
     9721 gaaggcatag acgtacgcgc cgatgctttc aggaatgacg cgggagatgt tgtcaggctt
     9781 tttggtgcag gaaactgtgg acgaaatcac agcgaaaacg gcgagcgaaa gcaagagtac
     9841 gctcattgaa atgaatggtt tgcgcatggc atgtttggtt ttgaatacgg ggaaaaaaag
     9901 tgaattcatt tttagattgt caacaggcga atccaacgga aaaggtaagc actttcttac
     9961 tttttatgtt actattgcaa cgaaaaaaaa aaatttggtc atgctgcctc tttttcctct
    10021 gcaaattgtg gtgtatcccg gcgaaaaact caacctgcac atcttcgaac cccggtacaa
    10081 acagcttatg caagcctgtg aaaatgaaaa cattacattc ggggtagtgc cctttctcga
    10141 cgggcggctt acgagcttcg gcaccgaggt tgtgctcacc gaagtacaaa agagatatga
    10201 caacggggag ttggatgtgc gaacacaagg catcgggctt tttcatttgg atgaattttt
    10261 caaaaccgct ccaggcaagt tgtatcccgg cggagatgtt acacgggtgg acattgtgta
    10321 tgacggcaca agaaagctgg gaggccacct tctgagcatg gcgcgctccc tgttcagcat
    10381 gttgtctatt gaaaaagaac tgcccgccga cccggatcgc tttttagctt atgacatcgg
    10441 gcactatgta ggattgtcgc aacaacagga atacgagttg ttgtgcatcg ccgaggaaaa
    10501 cgagcgccgc gagtacctca ttgagcattt ggaacagttg cttccattgg tacgcgaaac
    10561 agaggaactc aaacgcaagg tcagaatgaa cggacatttc agaaacctta caccgccgga
    10621 gtttttatag ccgtatcaat tgagtaaacg atgagatttt tttcagaaac gactgctttc
    10681 tggagagaag cggaaaaaaa atatgtgagt gaggtttggt tttttactnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cttctaaagt
    10801 tctaacaatt agcatgttaa ttgaaagagc tcgatcacac taagacaaat ttcctaccct
    10861 gaaaattgag ttgaccttca cctaattgct cgttttgcgg cagcaagtgg ttttttcgtg
    10921 taatcgaaat tgcgctgcat attgaggaag tagatggagg gctatgttac ctgaaacatt
    10981 ttttacgtcg aaatttcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nncttaacat ctaaaggcga cgtttcattc acacctgttt ccgccggaat tgacctatcc
    11221 ttcatcggtc cgatggcgcc taactgccca ttgaccgccg acccggtatg cggcaacaac
    11281 ggtgtaactt acctgaacgc ttgctttgca caggcagccg gtattacgca gtatgcttcg
    11341 ggggtttgct tcagcacctg tatcaacccg gcggccatcc agttgaacgc cgtttgtacg
    11401 ca
//
LOCUS       DAORLF010000100        11395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3041_length_11395_cov_1.936596, whole genome shotgun sequence.
ACCESSION   DAORLF010000100 DAORLF010000000
VERSION     DAORLF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_11395_cov_1.936596"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..835)
                     /locus_tag="PK198_09955"
     CDS             complement(35..835)
                     /locus_tag="PK198_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRF39102.1"
                     /translation="MAAEAKEREERHRKEEAQRQAKEKEEQKRREEAVQAFIEMVPVE
                     GGPFQFGGEHTVTVPDFEIGKYPVTQKQWREIMGNNPSRFKGDDLPVESVNWDDVKAF
                     IQKLNERFPGRHFRLPSEAEWEYAARGGRLSKGYEYAGSNKLDEVGWYWENSGDKPLS
                     GEWDRKKIEKNNCRTHPVGHIKANELGIHDMSGNVWEWCADDWHADYKGAPTDGSAWV
                     DSPQRGSFRVLRGGSWGDFAGDCRVSYRGSFSPGYRGDACGFRVALVP"
     assembly_gap    882..917
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<918..>1450)
                     /locus_tag="PK198_09960"
     CDS             complement(<918..>1450)
                     /locus_tag="PK198_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39103.1"
                     /translation="KKRAGSARFVPVLVRDCMWKLNPDIAEIQLLPRGDNGRIQGVAA
                     WLSRDSAWTNVCEELHKLIQEIHAHLEKEKAAAERARKAEEEDRQRQEAAEKAARALR
                     RRDEAFWKKISEDATRSDDPQHQIELYESYLQDDAFSLHRAEAEEAIETIQAEIEAAE
                     RLEVARRTAEEKRKAAE"
     assembly_gap    1451..1533
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1534..1716)
                     /locus_tag="PK198_09965"
     CDS             complement(<1534..1716)
                     /locus_tag="PK198_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39104.1"
                     /translation="MTATPLKTFIIYARADAAFKDELLNHLHLFVENRLIEKWVDSDL
                     LPGEDWEKRIEQELEAA"
     gene            complement(1890..2618)
                     /locus_tag="PK198_09970"
     CDS             complement(1890..2618)
                     /locus_tag="PK198_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRF39105.1"
                     /translation="MALPYIDIALPGIPPYRLMHVPGGEFLMGADEADTEAQDREKPA
                     HRVRLDDFYIGRFPVTQDLWEAVMRENPSRFKGPRRPVETVSWNDITEQFLPRLQETT
                     GGRYRLPTEAEWEYAARGGPFWKEEDFRYAGSEVLQQVGWYDENSGDATRDVGLLLPN
                     ALGLYDMSGNVWEWCADDWHSDYNGAPTDGSAWVDSPERGSRRVLRGGSWSNSAVRCR
                     VSYRIISDPVNRNGDFGFRVVLFP"
     gene            complement(2708..4213)
                     /locus_tag="PK198_09975"
     CDS             complement(2708..4213)
                     /locus_tag="PK198_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF39106.1"
                     /translation="MEIPTLTKKTFAPESLEKWFQPFRESIIGIHRQFETPYGEKKII
                     YADWTASGRMYGPIEDIMLHQVAPLVGNTHTETTVTGSSMTQAYHHAKHIIKHHVRAH
                     EKDVLIATSSGMTGVVNKFQRILGLKVHERFQHLLKLSEQERPVIFVTHMEHHSNQTS
                     WLETIADVVVVEPDADGLVDLARFEAVVQQYADRETKIAAVTSCSNVTGIATPYHDIA
                     ALMHRHGGLCFVDFACSAPYIDIDMRPEDEARHLDAIYFSPHKFLGGPGSSGVLIFDP
                     QLYKIRIPDSPGGGTVDWTNPWGEHKYIDEIEAREDGGTPAFLQTIRVAMAIQLKEQM
                     GVANMQRREEELLERLWAKFDRLQNLHVLADHVRRRLGVISFYVDGLHYNLGVKLLND
                     RFGIQVRGGCSCAGTYGHYLLHVSKQDSKSITEKINHGDLSAKPGWIRLSIHPTTSNE
                     EMDYILDAIEALCTHHQEWAKDYRYDVHTNEFQHLSGKDGIGEMVSGWFEW"
     assembly_gap    4335..4469
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4470..5153
                     /locus_tag="PK198_09980"
     CDS             <4470..5153
                     /locus_tag="PK198_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRF39107.1"
                     /translation="LSAAYRTLKTAYPLRPPKLLILSIMKPQKNTSHQYLTLYKPYGM
                     LSQFTAEAPGQSTLADWQPPLPKGVYPVGRLDKDSEGLLLLTDDARLNERLLHPRFGH
                     RRTYRVQVEGIPTTEALEGLRHGVSIKTDKGVYTTLPAEVALLTEAPKVPERVPPVRF
                     RAAIPTSWLDITLTEGKNRQVRKMCAAVGFPVLRLIRIRMEELELGGLLPGEQKSWER
                     EEIWRLLKM"
     gene            5209..5910
                     /locus_tag="PK198_09985"
     CDS             5209..5910
                     /locus_tag="PK198_09985"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRF39108.1"
                     /translation="MKKIVILSGAGMSAESGIRTFRDADGLWEQHDVMEVASIDGWRR
                     NPELVLRFYNQRRRQLKEVLPNAGHLALTRLEAAYDVHIVTQNVDDLHERAGSSNILH
                     LHGELNKVRSTGKTSLVYEWQDDLQMGDLCESGHQLRPHIVWFGEMVPLMDKAVEILT
                     KADIVIIIGTSMQVYPAAGLIAYARPSAAIYYIDPQPSINYELSDRRRLEVISDRAAA
                     AVPVLVERLLAEAGE"
     gene            6047..>6766
                     /locus_tag="PK198_09990"
     CDS             6047..>6766
                     /locus_tag="PK198_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39109.1"
                     /translation="MRKHVHHLIAIAAICLWASASYAQDAKIRRAEEAFKLLRYKEAI
                     QLYEEVLAKKEDLQVRLSLALAYRKLQDYERAAATFSKIKDWSNISPDYMLQYGCVLV
                     RSESCEDAQSWFDAYLARNPHDSRAPMFRNACAAMQQLMVCNEGLYEVFPMPFNTEQS
                     EMAPMIYGNQLVFASDRNAEQGPFLDLFSADRFDSTAQAQVFAPELNTRLHEDTAFFN
                     SSGNQVFFTRSRAGNKNLTDSR"
     assembly_gap    6767..6915
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6916..8224
                     /locus_tag="PK198_09995"
     CDS             <6916..8224
                     /locus_tag="PK198_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF39110.1"
                     /translation="DMPGGFGGKDLYYSEKTSIGWGMPINLGPEINTRGDEIFPFIGP
                     DNRLYFSSDGHPGLGGQDIMVSTLQPSGNWTNGENIGTPFNSQYDDFSFILAPDGLTG
                     YFASSRPGGAGRDDIYGFKLLYRQLSLAFQNSVTGEAIEEASVKTRCDMLPNHSLRVP
                     VYACCNLTVSAPGYDDAAVEICGEDTRVDYSRPYPVALQPERKYTLRGTATDGYTGAP
                     LSNAVVLIFEAGNYFNSVVSGADGAFMYELPKNKCYTFKVEKGDYFSRSLADTICTTG
                     KTFDFSLQSALQPFRANSSRTGSGPAKGVFIPGIPTGDDRAAPFVLQIYYDLHSTRFR
                     DDNLPEMERLLRLLQDNPNISVEISSHTDTRGEADFNLRLSQKRAELVVEWLIAKGID
                     GRRLMAKGYGEARPVNQCLDGVTCPEAEHQLNRRTEFRVLGNR"
     assembly_gap    8268..8277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8296..8721
                     /locus_tag="PK198_10000"
     CDS             8296..8721
                     /locus_tag="PK198_10000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRF39111.1"
                     /translation="MYSHDLQLRVRYGETDQMGYLYYGNYAQYYEIGRVEMLRASGLT
                     YREMEQERGVLMPVVSLQMRYVRPAYYDELLTIRTTLRRLPEKFITFEVEIFNENRKL
                     VNGGSVRLCFVDAKSGKTIAAPEFLLEKLRAHFEKAPTT"
     gene            8699..9694
                     /locus_tag="PK198_10005"
     CDS             8699..9694
                     /locus_tag="PK198_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRF39112.1"
                     /translation="MKKPQLPDIEQWKRRLMRARWMRKLLVWAKRRSLPGFSRVPIYD
                     VVVFTTNEIQRFDLFLRANAIAYSFFLALFPALISLFTLLPHLRKYFLHYLPNEGDHF
                     EDVLQHEIQQIVPGDVGLHISNFIEDLTTNPRVGLLSFGFFLALFFASNGMLALMQGF
                     EKSYARTFIKRNALSKRGIASMLTFLLAFLLLASVVLIILGNQIIHAAGAWTGWNRVT
                     EWSISILRWLTIVSVYYFGIAIIYRFGAAVRRKFRWFSPGATLATILCLLTSLIFSFY
                     VDNFGNYNKLYGSFGTIIVTMLWLQLNALGLLIGFELNAGIAINRDLKQAREDER"
     assembly_gap    9913..9951
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9952..10194
                     /locus_tag="PK198_10010"
     CDS             <9952..10194
                     /locus_tag="PK198_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39113.1"
                     /translation="SGQVHYRSHEARFSMYYELGGGETVAGIFIPDAESWETATSLPL
                     FRREEVLESIGRQVVTDKTGGRGHFKIEGYWLQIYH"
     gene            10208..11284
                     /locus_tag="PK198_10015"
     CDS             10208..11284
                     /locus_tag="PK198_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39114.1"
                     /translation="MLGNILLVFITLALIAFVQLTSSPAPGGDRGVGYAFAMILYGGA
                     LFLFSGLLAWYAGKNGLFNWAPLAPQNRNLLIFTGWLAFFFAVTLSGMFRSEWHPGEL
                     PQFLKWLADAQAAVWLPLLVLVPLAVFINSEKQVAAPELWVKIPFLAGMGICAIIGLT
                     MLFGWFRASARQNAEQAEYHQQREQSQHQHHLDKIAAHQPEDPLVNILSLTGRYHDAD
                     VRNAAVEKVKSRPDWEAELIRLLNDTEWQSYVYQFIDGNEVPHPELFVEPINRSIRRT
                     AAEIRSRITDANDLQDWHFEHFSIERLLRAIDEQFSKVPGADFRPAMLELLAALKTPK
                     PERFKKVEFTLTPIVEYWLRGNKK"
BASE COUNT         2324 a         3161 c         2919 g         2539 t
ORIGIN      
        1 tccagggcag caccggaaag gcaggccacc gaagctaagg gacgagggcc acgcggaagc
       61 cgcaggcgtc gccgcggtag cccgggctga agctgccccg gtacgacaca cgacagtccc
      121 cggcgaagtc gccccacgag ccgccgcgga gcacgcggaa cgagccccgc tgcgggctgt
      181 ccacccaggc gctgccgtcc gtcggagcgc ccttataatc ggcgtgccaa tcgtccgcgc
      241 accattccca aacattcccg ctcatgtcgt gaatacccaa ctcgttcgcc tttatatgac
      301 cgacaggatg tgtgcgacag ttgttctttt caattttttt ccggtcccat tcacccgaca
      361 aaggcttatc cccggaattc tcccaatacc accccacttc atccagctta ttgctaccgg
      421 catactcgta tcccttcgac agccgcccgc cccgggccgc gtactcccac tccgcctcac
      481 ttggcaaacg gaaatgccgg ccaggaaacc gttcattcag tttttgaata aatgctttta
      541 catcatccca attgacgctt tccacgggca ggtcgtcgcc tttgaaacga gagggattgt
      601 tccccatgat ctcccgccat tgtttttgcg tcacggggta tttgccgatc tcgaaatcgg
      661 gcaccgtgac cgtatgctcg ccgccgaact ggaaggggcc tccttccacg ggtaccattt
      721 ctataaacgc ctgaacagct tcttcccggc gcttttgttc ttccttttct ttggcttggc
      781 gctgagcctc ctctttcctg tggcgctcct cgcgttcttt ggcctcggca gcaatgcggg
      841 cggcttcggc ttcgcgctcg gccgcttcct tgcgtttttg tnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnttc ggcggctttg cgcttttctt cggcggtccg gcgggcgacc
      961 tccaagcgct cggcggcttc tatttcagcc tgtatggttt cgatggcctc ttcggcctcg
     1021 gcgcgatgca gagagaaagc atcgtcctgt aggtagcttt cgtacagttc tatttggtgc
     1081 tgcgggtcat cgcttcgtgt ggcgtcttcc gagatttttt tccaaaaggc ttcgtctcgg
     1141 cggcggaggg cgcgtgctgc cttttcggcg gcttcctgcc gttgccggtc ttcctcttct
     1201 gctttgcggg cgcgctcggc agcggctttt tccttttcga ggtgggcgtg gatttcctgg
     1261 atgagtttgt gcagttcttc acacacattg gtccaggcgc tgtcccggct caaccaggca
     1321 gctacgccct gaatccggcc attgtcgccg cgaggcaata attgaatttc agcaatatcg
     1381 gggttcaatt tccacataca atcgcgcacc aacaccggaa cgaagcgggc gctgccggcg
     1441 cgcttttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgccgct tccaactcct gctcaatgcg
     1561 cttttcccaa tcctcgccgg gcagcaggtc ggagtccacc cacttctcta tcagcctgtt
     1621 ttccacaaac aggtgcaggt ggttgagcaa ctcatccttg aacgctgcgt cggcgcgggc
     1681 atagatgatg aaggtcttaa gcggagtagc ggtcatagtc ctgcaaaagt agggtattgt
     1741 ggaaaatatg gaagaatttt gtttgagcga aaatatctgc tgggactccc ttccgcgacc
     1801 cagccggaac agtattccgg cagacattcc tgctcactcc ccggccggct ttagctccag
     1861 ggcagcaccg gataggtagg ccaccgaaac taagggaaga gaactacgcg gaaaccgaag
     1921 tcgccgttgc ggttgaccgg gtcgctgatg atccggtacg acacgcgaca gcgcacggcg
     1981 ctgttgctcc acgagccgcc gcggagcacg cggcgcgatc ctctctccgg gctatccacc
     2041 cacgcgctgc cgtcagtagg ggcaccatta taatcgctat gccaatcgtc cgcgcaccac
     2101 tcccacacat tcccgctcat atcgtacagg cccagcgcat tgggcagcag caggcccaca
     2161 tcgcgggttg cgtcgccact gttttcatcg taccagccca cctgctgcaa cacctcactc
     2221 cctgcgtagc ggaagtcttc ttccttccaa aacgggccgc cacgagcggc atattcccac
     2281 tccgcttcgg tgggcaggcg gtatcggcct ccggtagttt cctgcagccg gggcagaaat
     2341 tgttcagtga tgtcgttcca ggaaactgtt tccaccggcc gacgcgggcc tttgaaacgg
     2401 gaagggtttt ctctcatcac cgcctcccag aggtcttgcg tcacgggaaa gcggccgatg
     2461 taaaaatcgt ctaaacgcac gcggtgggcg ggcttttccc ggtcttgcgc ttccgtgtct
     2521 gcctcatcgg cgcccatgag gaattcgccg ccgggaacgt gcatcaggcg gtagggcggt
     2581 atgccgggca gggcaatgtc tatgtagggt aaggccatat aatcattttt ttcttttctc
     2641 ctgcgtagtg aaggggtgac tacggtagca ttgcagggac aagtcacccc ttcactcaac
     2701 tcccccctca ccactcaaac cacccgctca ccatctcccc gatgccgtcc ttgcccgaaa
     2761 ggtgctgaaa ctcattggta tgcacatcgt agcggtagtc tttggcccat tcctgatggt
     2821 gggtgcagag ggcttctatc gcgtccaaga tgtagtccat ttcctcgttg gaagtggtgg
     2881 ggtggatgga gaggcgaatc cagccgggct tggcagataa gtcgccgtgg ttgatttttt
     2941 ccgtgatgga cttggaatcc tgcttcgata catgcagcag gtagtgcccg taagtgccgg
     3001 cgcaggagca gccgccgcgc acctgtatgc cgaagcggtc gttgagcagt ttcacgccga
     3061 ggttgtaatg cagaccgtca acataaaaag agatgacgcc caatcggcgc cgcacatgat
     3121 cggccaaaac gtgcaggttt tgcaggcggt cgaacttggc ccagaggcgc tccagcagtt
     3181 cctcctcgcg gcgctgcatg ttggccacgc ccatctgttc tttcagttgg atggccattg
     3241 ccacccggat ggtttgtaaa aaagccggcg tgccgccgtc ctcgcgggcc tcgatttcgt
     3301 caatgtattt gtgctcgccc cagggattgg tccagtccac cgtgccgccg ccgggactgt
     3361 cgggaatgcg gattttgtac aattgggggt caaaaatgag tacgcccgat gagccgggac
     3421 cgccgagaaa cttatgcggc gaaaaataaa tggcgtccaa atgccgggcc tcgtcttcgg
     3481 gccgcatgtc aatgtctatg tacggcgccg agcaggcaaa atccacaaaa cacaggccgc
     3541 cgtgccggtg catgagcgcg gcaatatcgt ggtaaggcgt ggcaatgccc gtcacgttgg
     3601 aacaggaggt caccgccgcg attttggttt cccggtcggc gtattgttgt accaccgctt
     3661 caaagcgcgc caggtcaacg aggccatccg catcgggttc caccaccacc acatcggcga
     3721 tggtttcgag ccaggaagtc tgattggaat ggtgctccat gtgggtcaca aaaatgacgg
     3781 ggcgctcctg ttccgacagt ttcagcaggt gctgaaagcg ctcgtgtact ttgaggccca
     3841 gaatgcgctg gaatttattg accacgcccg tcatgcccga actcgtggcg atgagtacgt
     3901 ctttttcatg ggcgcgcaca tggtgtttga tgatgtgttt ggcgtggtgg taagcctggg
     3961 tcatgctgga gccggtcacg gtggtttcgg tatgcgtatt gcctaccaaa ggcgctacct
     4021 gatgcaacat gatgtcttcg atggggccgt acatgcgccc gctcgctgtc cagtcggcat
     4081 aaatgatctt tttttcgcca taaggcgttt caaactgccg gtggatgccg atgatgcttt
     4141 cgcgaaaggg ctgaaaccat ttttcgagcg attccggcgc aaaggtcttt ttggtaaggg
     4201 tgggtatttc catgtttgca aagatttcgg attcaagatt tcggattctc tggtaggagc
     4261 ggctgccggc cacaagcctc aagctgctgg ctgctggcct caagctgctg gctgcgttca
     4321 ccgcgctttc gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcagcgccgc ctaccgcaca ctgaaaaccg
     4501 cctaccccct acgaccgcca aagttactca ttctctccat tatgaaaccg caaaagaaca
     4561 caagccatca atacctgacg ctgtacaaac cctacggcat gctctcgcaa ttcactgccg
     4621 aagcgcccgg acaaagcacc ctggccgact ggcagccgcc tttgccgaaa ggcgtttacc
     4681 ccgttggccg tttggacaaa gacagcgagg gcctgctgct gctcaccgat gatgcacgcc
     4741 tcaacgagcg cctgctgcat ccccgtttcg ggcatcggcg tacctaccgc gtacaggtgg
     4801 agggcattcc tacaacggag gctttggaag gcttgcgaca cggcgtttcc atcaaaaccg
     4861 ataaaggcgt ttacaccacg ctgcctgccg aagttgcgtt gctgaccgaa gcgccgaaag
     4921 tgccggagcg cgttcctccg gtgaggttcc gggccgccat tcctacctcc tggctcgaca
     4981 tcacgctcac cgagggcaaa aaccggcagg tgcgcaaaat gtgtgccgcc gtgggatttc
     5041 cggtgctgcg cctcatccgc attcgcatgg aggagctgga attgggcggt ttgcttcccg
     5101 gagagcagaa aagttgggag cgggaggaga tttggaggct gttgaaaatg tagggtgtag
     5161 ctgcgagcga ttggggccta tttatttcaa accacttatc tttgccgcat gaaaaaaata
     5221 gtcatcttat cgggcgccgg catgagcgcc gaaagcggta tccgcacctt ccgcgatgcg
     5281 gacgggctgt gggaacaaca tgacgtgatg gaagtagcct ccattgacgg ctggcgacgc
     5341 aaccccgaac tcgtgctgcg attttacaac cagcgccgcc ggcaactcaa ggaagtattg
     5401 cccaacgccg ggcacctcgc tctcacccgc ctcgaagctg cttacgacgt gcatattgtg
     5461 acccaaaatg tggatgacct gcacgagcgc gccggcagca gcaacatcct gcacctgcac
     5521 ggcgaattga acaaagtacg cagcaccggg aagacttcat tggtgtacga gtggcaggat
     5581 gacctccaaa tgggcgacct gtgcgagagc ggacaccagc tccggccgca catcgtttgg
     5641 tttggcgaaa tggtgcccct gatggataag gccgtagaaa ttctgaccaa agccgacatc
     5701 gtcatcatca tcggtacttc catgcaggta tatcccgccg cgggtctcat tgcttatgcg
     5761 cgcccttcgg cagccattta ttatattgat cctcagccat ctatcaatta cgaactgagc
     5821 gaccgccggc gcctggaggt tatttccgac cgggccgctg ctgctgtgcc ggtattggtg
     5881 gagcgattat tggcggaagc cggagagtaa tttgacaaat gaaaaaacct ttgtattttt
     5941 gcgcttgcta ttgaacggca cacacataag aaccagcctt gggggatttt tttattaaga
     6001 caggctgaac gacatacaag atttactcac acagagccga tttgttatga gaaaacatgt
     6061 acaccacctt atcgccattg cggcgatatg cctttgggca tctgcctcct acgctcagga
     6121 tgcaaaaatc cgcagagcag aagaagcctt caaactcctt cgctacaaag aagccattca
     6181 gttgtacgag gaagtgctgg ccaaaaaaga agaccttcag gtgcggctca gcctggcctt
     6241 ggcttatcgc aaactgcagg actacgaacg cgctgccgcc actttttcca aaatcaaaga
     6301 ctggtcgaac atctcgccgg attacatgct gcaatacggc tgcgtgctgg tgcgctccga
     6361 gtcctgcgag gatgctcaat cctggttcga tgcctatttg gcgcgcaatc cgcacgactc
     6421 ccgcgcgccg atgtttcgca atgcctgcgc cgccatgcaa caattgatgg tatgcaatga
     6481 gggcttgtac gaggtcttcc ccatgccttt caacacggaa cagagcgaaa tggcgcccat
     6541 gatttacggc aaccaattgg tttttgcttc cgaccgcaac gccgagcagg ggccgttcct
     6601 cgatcttttt tcagccgacc ggttcgacag cactgcacag gcacaggtct ttgcgcccga
     6661 actcaatacc cgcctgcacg aggatacggc ttttttcaac agcagcggca atcaggtttt
     6721 tttcacccgc agccgcgccg gcaacaaaaa cctcaccgac agccgcnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnggata tgcccggagg atttggcggc aaagacctct attattctga
     6961 aaaaacgagc atcggctggg gaatgcccat caacctcggc ccggaaatca acacccgcgg
     7021 cgatgaaatt ttccccttta tcgggccgga taatcgcctg tatttcagct cagacgggca
     7081 tccggggctg ggcgggcaag acatcatggt atcaactttg caacccagcg gcaactggac
     7141 caacggagaa aatataggca ctcctttcaa ctcgcagtac gacgactttt ccttcattct
     7201 ggcccccgac ggactgacag gctacttcgc ttcctcgcgg cccggcggcg caggccggga
     7261 tgatatttac gggttcaaac tgctgtatcg ccaattgagt ctggcgttcc aaaacagcgt
     7321 caccggcgag gccatcgaag aggcaagcgt caaaacccgc tgcgacatgc tgcccaacca
     7381 ttccctgcgc gtgccggtgt atgcctgctg caacctcacg gtttcggcgc ccggctacga
     7441 tgatgctgcg gtggaaattt gcggagaaga tacccgtgtg gactactcgc gcccctaccc
     7501 tgttgccttg cagcctgagc gaaaatatac cctgcggggc acggctaccg acggctacac
     7561 gggcgcgcct ttgtcgaacg ccgtagtgct catttttgaa gccggcaact atttcaattc
     7621 cgtcgtttca ggcgcagatg gagcatttat gtacgaactg cccaaaaaca agtgctatac
     7681 cttcaaagtg gaaaaaggcg attatttctc ccgtagcctt gccgatacca tttgcaccac
     7741 aggaaaaact tttgatttca gcctgcaatc agcgctgcaa ccgttccggg ccaactcctc
     7801 gcggacgggt tccggccctg ccaaaggcgt gttcattccc ggcattccta ccggcgacga
     7861 ccgcgcagcg cccttcgtac ttcaaattta ttacgacctg cacagcaccc gtttccgcga
     7921 cgacaacctg cccgaaatgg agcgcttgct gcgtttgttg caagacaatc ccaatatttc
     7981 ggtggaaatc agttcgcata cagatacgcg cggagaagcc gacttcaacc tgcgcttatc
     8041 gcaaaaacgc gccgaattgg tggtggaatg gctgattgcc aaaggcatag acggtcgcag
     8101 gctgatggcc aaaggatacg gcgaggcccg tccggtgaat caatgcttag acggcgtgac
     8161 ctgcccggag gccgagcacc aactgaaccg gcgcacagag ttcagagtat tgggcaatcg
     8221 ttgataactt atctttttgt gtcggattct aagtatcttt gcgctccnnn nnnnnnnatc
     8281 attcatcatt aacacatgta cagccacgac cttcaactcc gggtgcgcta cggcgaaacc
     8341 gatcagatgg gctacctcta ctacggcaac tatgcccagt actacgagat aggccgcgta
     8401 gaaatgttgc gcgcatcggg gctgacctac cgcgaaatgg agcaggagcg cggcgtgctg
     8461 atgccggtag tatccttgca aatgcgctat gtgcgaccgg cttactacga cgaattgctg
     8521 acgatacgca ccacgctgcg ccggttgccg gaaaaattca tcactttcga ggtcgaaatt
     8581 ttcaacgaaa accgcaagtt ggtcaacggc ggctcggtgc ggctgtgttt tgtggatgca
     8641 aaaagcggca aaaccatcgc agcgcctgaa ttcctacttg aaaaactgag agcgcatttt
     8701 gaaaaagccc caactacctg atatcgagca atggaagcgc cgcctgatgc gtgccaggtg
     8761 gatgcgaaaa ttgttggtgt gggccaagcg ccgctcactg ccggggtttt cgcgtgtgcc
     8821 catttatgat gtggtggttt ttacaaccaa tgaaatacag cgtttcgact tgtttttgcg
     8881 ggccaatgcc attgcgtaca gcttctttct ggctttgttt ccggcgctca tttccctttt
     8941 tacattgctg cctcatctcc gcaagtactt cctgcactac ctgcccaacg agggcgatca
     9001 tttcgaggat gtgcttcagc atgaaattca gcagattgtg ccgggcgatg tggggctgca
     9061 catatcaaat tttatagaag acctgaccac caatcccagg gtaggtttgt tgtctttcgg
     9121 tttttttctg gccctgtttt ttgcctccaa tggtatgttg gctctcatgc agggttttga
     9181 aaaatcgtac gcccgcacct tcatcaaacg caatgcgctc agcaaacggg gcatcgcgtc
     9241 catgctcact tttctgttgg cgtttttgtt gctggcatcg gtggtgctca tcattctggg
     9301 caaccagatc atccacgcgg caggcgcctg gaccggctgg aaccgcgtga cggagtggag
     9361 catttccatc cttcgctggc tcactattgt gtcggtttat tatttcggca tcgccattat
     9421 ttacaggttc ggcgcggccg tgcgacgcaa attccggtgg ttctcgccgg gcgccacgct
     9481 ggccactatt ttgtgcctgc tcacctcgct gattttttct ttttatgtgg ataattttgg
     9541 caattacaac aagctctacg gcagtttcgg caccatcatt gtgacgatgc tgtggctcca
     9601 actcaatgca ctggggctgc tcatcgggtt tgaactcaac gcgggcattg ccatcaacag
     9661 agatttaaaa caagcccggg aagacgaacg atgaaaaaaa acgcgaactt tgcccctaca
     9721 acctaaaatt cattatctgc catgtgccaa ctattcttgt acaaattccg tgctcccatt
     9781 cccgaatctg tgcgaaattc gccctggctc tggattgggc tggcagcggt ggtcatagga
     9841 agtctggtat atcacaattt tttcagaaaa acacgagtct cgcccgctcc aacgcacccc
     9901 gcagcttccg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcgggcag
     9961 gtgcattacc gcagccatga ggctcgtttt tcgatgtact atgaactggg cggcggcgaa
    10021 acagtagcgg gcatttttat tcccgatgca gaaagctggg aaactgccac ttctcttcct
    10081 ttatttcgcc gggaagaagt gttagaaagc attggtcgcc aagtggttac agacaaaacc
    10141 ggcggacgcg gtcattttaa aatagaggga tactggctcc agatttatca ttaaaatcca
    10201 aaacaccatg ctcggaaaca tcctcctcgt cttcatcacc ctggccctca ttgccttcgt
    10261 tcagctcacc tcctcgcccg cgcccggcgg agacagaggc gtcggctacg cttttgccat
    10321 gatcctatac ggcggcgcat tgtttctgtt cagcggctta ttggcctggt atgccggcaa
    10381 aaacggcctg ttcaactggg cgcctttagc tcctcaaaat cgcaatctgc tcatttttac
    10441 gggctggttg gcgtttttct ttgccgttac actcagcggc atgtttcgca gcgaatggca
    10501 ccctggcgag ttgccgcaat ttctcaaatg gttagccgat gcacaggctg ctgtctggct
    10561 gcccctcttg gtactcgtgc cgctggcggt attcatcaac tcggaaaaac aggtggcagc
    10621 gcccgaactt tgggtcaaaa ttcctttcct cgccggcatg ggcatttgcg cgatcatcgg
    10681 gctgaccatg ctgttcgggt ggtttcgggc ttctgccaga caaaacgccg aacaggctga
    10741 gtatcatcaa caacgggagc aatctcagca tcaacaccat ttagacaaaa tagccgcgca
    10801 ccagccggaa gacccattgg taaacatcct ctcgctcacc gggcgctacc acgacgccga
    10861 tgtgcgcaat gccgccgtag aaaaagtaaa atcacgcccc gactgggaag ccgaactcat
    10921 tcgcctgctc aatgatactg agtggcaatc gtatgtgtat cagttcattg acggcaatga
    10981 ggtgccgcat cccgaacttt tcgtcgaacc catcaaccgc agtatccgcc ggacagccgc
    11041 cgagattcgc agccgcatta ccgacgccaa cgatcttcag gactggcatt tcgagcattt
    11101 cagcatcgag cgcttgttgc gcgccataga cgagcagttt tccaaagtgc ccggcgccga
    11161 tttccggccc gccatgctcg aactgcttgc agctttgaaa acacccaaac cggaacgttt
    11221 taaaaaagta gaattcaccc tcacgccgat tgtggaatac tggctaaggg gtaataaaaa
    11281 ataaagtgtt cattttcgtc aaaggtgact atacagtcgc cctgcaaaaa gccgctagcc
    11341 gcgagctttg agtcacgagt ttgatttccc aaaataaaaa aagggccttt tttca
//
LOCUS       DAORLF010000101        11363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3052_length_11363_cov_2.716926, whole genome shotgun sequence.
ACCESSION   DAORLF010000101 DAORLF010000000
VERSION     DAORLF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_11363_cov_2.716926"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..>691)
                     /locus_tag="PK198_10020"
     CDS             complement(296..>691)
                     /locus_tag="PK198_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRF39115.1"
                     /translation="NPFELHTSWHFPFFERLDMDLVQQAAALLPQYELFFPFCKSETD
                     AKTMRCALSRSEWVLDEARHRLVFHISADRFLRGMVRLIVGACLNAGLGQVSLDEIRK
                     ALEQQTRIAKSWSIPPDGLFLTEVKYPYG"
     assembly_gap    692..701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<702..1041)
                     /locus_tag="PK198_10025"
     CDS             complement(<702..1041)
                     /locus_tag="PK198_10025"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRF39116.1"
                     /translation="MRYFAELAYNGTQYCGWQRQPNGMSVQEKIETALSTLLREQVEV
                     TGCGRTDAGVHASQYVMHFDLENEFQPEFLGRLNRMLPPDIALYRVWPVAPEAHARFD
                     AVLRSYEYHIS"
     gene            1307..2494
                     /locus_tag="PK198_10030"
     CDS             1307..2494
                     /locus_tag="PK198_10030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRF39117.1"
                     /translation="MTIKTAILHKGKDAPLRRRHPWVFSGAIRRWEGRPQDGEVVEVR
                     SADGELLGTGHFQDGSIQVRVFAFEQVEVNTDFWTAKLNNAFELRRRLGLPQAGHTDC
                     YRLIHGEGDGLPGLIIDVYGRTAALQCHSIGMHLHRHELAEALHRTLGENLLAVYDKS
                     ADSLPSAYGAAVTPGYLSGHNEPAPVLENGHAFEVDWEGGQKTGFFLDQRDNRQLLAQ
                     YAAGQTVLNAFSYTGGFSVYALAAGARLVHSVDISGKAVEAAVRNAALTGVPADRHEG
                     IVSDVLKYLQTSEDGLYDIMVVDPPAFAKSTEKRHNAIQAYKRLNALAMRKIKPGGLL
                     FTFSCSQVVDRTMFYNTMVAAAIEAGRAVRVLHHLRQPADHPVSLFHPEGEYLKGLVL
                     EVE"
     gene            2597..3052
                     /locus_tag="PK198_10035"
     CDS             2597..3052
                     /locus_tag="PK198_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF39118.1"
                     /translation="MKKMMIATLALMSVAFTTLEAQKKTPGQEAVSSDVVESLPRFPG
                     CEDQADEKIKIACSNGKLAEFIAQNIAYPPKAFKKGIQGKPVVSFTVEKDGSLSNIKV
                     LREIGGGCGKEAARVIQLMNDRGLRWIPGMQNGTPVRVTYSLPIGFKLQ"
     gene            3110..>4372
                     /locus_tag="PK198_10040"
     CDS             3110..>4372
                     /locus_tag="PK198_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF39119.1"
                     /translation="MSTHTATLNNPRTINAWAFFDWANSAFALVITSAIFPAYFAAVT
                     NDQVRIFGIEMSNSSLYAYAVSASYLVIAICSPILSGIAYYGVRRMFFLKFFTIFGAL
                     GCLSLYLFRGMPSLAIGLTGFIVAMIGFAGGLVFYNSFLPQIVTEDRYDRVSARGFSF
                     GYVGSVLLLLVNLWMILNSEKIGFANEGVATRLAFVMVGLWWIGFAQIPFRRLPRDPK
                     DRPKQQLLTKGFKELAKVARIVAQSKDMTRFLGSFFCYSAGVQTAIFLAATFAEKELK
                     FESTELIIIILILQLVAIGGAYFFAKVSDWRGNKFSLMAMLVIWTGICFAANQVYTKT
                     QFYFLAAAVGLVVGGIQSLSRSTYSKLIPEGTKDTTSFFSFYDVLEKTAIIIGTFGFG
                     FIAQVTGSMRMSVLLLAAFFVLGMVILAS"
     assembly_gap    4373..4399
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4490..5535
                     /locus_tag="PK198_10045"
                     /pseudo
     CDS             4490..5535
                     /locus_tag="PK198_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            5555..6619
                     /locus_tag="PK198_10050"
     CDS             5555..6619
                     /locus_tag="PK198_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRF39120.1"
                     /translation="MSNPTTTVRPGEELPLDALSDYLLAHWPGFSQITALRQFPGGYS
                     NLTYLLETDAGAVVLRRPPFGAQIKSAHDMGREFRVLSLLQGHFDKIPQPVLICEDDS
                     IIGAPFYLMEQVQGVILRSKPPKEFQLSPDNMRRISEASVDTLAALHAIDLEASGLLQ
                     LGKPQGYIERQTTGWVERYRKAETDAIADMDAVAEWLTAHRPNDGAPAFLHNDFKYDN
                     IVLDPADPGRVVAVLDWEMATVGDPLMDLGTTLAYWTEAAEAEALPLAAANLTWLPGN
                     LRREQALERYAAQTGRNADDFLFFFVFGAFKIGVIVQQIYARYKKGYTQDARFAGLLD
                     AVRHFGRMGAGAIERGRMGG"
     gene            complement(6664..7089)
                     /locus_tag="PK198_10055"
     CDS             complement(6664..7089)
                     /locus_tag="PK198_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRF39121.1"
                     /translation="MLDTNVLLVSISSKSRYRPILEAFINEEFELCVTTDILLEYEEI
                     IKDHMGDRFAEYVLQLIENAPNVQWITKYYKWNLIETDADDNKFVDCAIACDAKYLVS
                     DDKHFNVLLKIPFPKVDVLTAQEFKERIEGKENAPQKDT"
     gene            complement(7104..7343)
                     /locus_tag="PK198_10060"
     CDS             complement(7104..7343)
                     /locus_tag="PK198_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39122.1"
                     /translation="MNAVPQLNSLNKHQLEILKLFSRELDDNDLIEIKRLIVKYLDQK
                     ITKMADDIWEKNNWTDEDMERILKTHKRTPYNPNN"
     gene            7818..>9655
                     /gene="mnmG"
                     /locus_tag="PK198_10065"
     CDS             7818..>9655
                     /gene="mnmG"
                     /locus_tag="PK198_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRF39123.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAVAAANLGSKVLLATMNMQTIAQMS
                     CNPAMGGVAKGQIVREVDALGGYSGIVTDETMVQFRMLNRSKGPAMWSPRAQSDRMLF
                     ARKWRQMLEAHPNIDFWQDMVNGLVVKDGRVCGVRTGMGLEIPCKAVVLTNGTFLNGI
                     IHIGEKQLGGGRAGESAARGITEQLVSLGFESGRMKTGTPPRLDGRSLDYSRMEVQPG
                     DEQPGRFSYTETTPLTAQMPCHITYTNDKVHEILRTGFDQSPMFTGRIQGLGPRYCPS
                     IEDKIDRFADKDRHQLFVEPEGRDTCEIYVNGFSSSLPEDVQFKALRLIPGFENARMF
                     RPGYAIEYDYFPPTQLKLTLETHLVKNLFFAGQINGTTGYEEAACQGLMAGINAHLNI
                     AEQEPFILKRSEAYIGVLIDDLINKGTQEPYRMFTSRAEYRILLRQDNADLRLTPLAY
                     QMGMMNMEARMERVRAKEEAAKAIERFLRDTSADPAELNGFLSELDSSPLRQKTKLHG
                     ILLRPNVSMAGLSRAVPALHHFLAGFDQESIELAEINLKYEGYIDKEREMADKLNRLE
                     EVKLPENFDYQPLVSLSKEAREKLSRIRPRTIGQAGRISGVTQADVS"
     assembly_gap    9656..9719
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9831..11159
                     /locus_tag="PK198_10070"
     CDS             9831..11159
                     /locus_tag="PK198_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF39124.1"
                     /translation="MEISVSLCCKDEKQYIGVNTVVFDAVFPARIRQIQGAYWSPALK
                     YWLLPYTSEHWNRLKYVFKDTDFIFQADFEPPQDSTLAATLPLPPEVVIEILLHPSSP
                     NLLRILLPKVLVPRWLASVRGIPGRKWNPETACWEVSYTRTSVAYIRQYLPPSLVHWH
                     FTPSEDLPDTPAPPVRRTPLPPASSQVVRPRYEAAATALEQTLLLKRYSYNTIKTYIL
                     YQIKTKNISESHQNMILSALKMFYAEVLGQHEKVRDMIRPKRPQKLPHTLTENEVSAL
                     LRAAANPKHKCILMLIYSAGLRLGELIRLRLKDMEPAAGRVFVYGGKGKKDRCTILSP
                     KVWEQLQTYIAVYRPVEWVFEGQTGGQYSARSVQELFTQAKIKSGINPYATVHTLRHS
                     FATHLLEAGVDLRYIQDLLGHESSKTTEIYTHITRVGWQRVQSPLDRLHL"
BASE COUNT         2766 a         3031 c         2883 g         2582 t
ORIGIN      
        1 cgcgcgcggg cgttgcgtca aaagcgcttg tgaagagaaa atggaggcga aaacgctcgc
       61 ctcagcgttg ctacttgtat gtataaatag cagagggact gttatctgat tttgtactct
      121 ataaatcggt gttttttaat ttttttctaa aacagcacca gccgcctgct aaaaactgcg
      181 gctacacaag gcgtggccgg gttcacccgt ctttcccgtt caggatatgg tttttcaaat
      241 actgaatggt gatgaccatg tcagctacta agggcaatcg ccggcccgaa aaaaatcatc
      301 catacggata cttcacctcg gtgagaaaca gcccgtcggg cgggatgctc cagcttttgg
      361 cgatgcgggt ttgttgttcc agggccttgc gtatttcgtc caaagagacc tggcccagac
      421 ccgcgttgag gcaagccccc acaataaggc gcaccatgcc gcgcaggaag cggtcggcag
      481 agatgtggaa taccaggcgg tgccgggcct catcgagcac ccactcggag cggctgagcg
      541 cgcagcgcat ggttttggcg tcggtttcag acttgcaaaa agggaagaac agttcgtact
      601 gaggcagcag ggcagcggct tgttggacga ggtccatatc caagcgctcg aagaagggga
      661 aatgccagct tgtatgcagt tcgaagggat tnnnnnnnnn ncagaaatat ggtactcgta
      721 gctgcgcagt acggcatcga agcgggcgtg cgcttcgggt gcaacgggcc atacccggta
      781 cagcgcaatg tcgggcggca acatgcgatt gaggcgaccg aggaactccg gttggaactc
      841 gttttcgagg tcgaagtgca tgacatattg cgaggcgtgc acccccgcgt cggtacggcc
      901 gcagcccgtc acttccacct gttcacggag gagggtcgag agggcggttt ctattttttc
      961 ctgcacggac atgccgttcg gttggcgttg ccagccgcag tattgagttc cattgtaggc
     1021 cagttcggcg aagtagcgca tgggatgatg agactatgag acggtgagat tgtgagactt
     1081 ccagacggtg agacggccgg tatcgcttca gttttgttat aaatactcag taaggtttaa
     1141 atgaaaaaag tttgtttcgg cggtttagtg aagggcgact gggccaagac caggccccgt
     1201 agtcacccct tcactacgca gagaaacttc atttacactg cactcagttg gaggcgtaaa
     1261 ggtacttttt tattagaaaa tcccgaaaac ccctaccttt cgccccatga ccatcaaaac
     1321 agccatacta cacaagggca aagacgcgcc cctgcgccgt cgccatccct gggttttttc
     1381 gggcgctatc cggcgttggg aaggccgccc gcaagacggc gaggtagtag aagtgcgcag
     1441 tgccgacggc gagttgctcg gcacggggca ttttcaggac ggcagcattc aggtgcgggt
     1501 attcgctttt gaacaagtag aagtcaatac cgatttctgg accgccaagc tgaacaacgc
     1561 cttcgaactg cgccgccgac tgggactgcc tcaagcgggc cataccgatt gctaccgact
     1621 gatacacggc gagggcgacg gtctgcccgg cctcatcatt gacgtatatg ggcgcacagc
     1681 ggccttgcaa tgccattcca tcggcatgca cctgcaccgc cacgaactgg ccgaggcact
     1741 gcaccgcacg ctcggcgaaa acctgctggc cgtgtatgac aaaagcgccg actcgctgcc
     1801 ctcggcttat ggcgctgccg tgacgccggg ctacctcagc ggccacaacg aacccgcgcc
     1861 ggtgctggaa aacggccacg ctttcgaggt ggactgggaa ggtggtcaaa aaaccggttt
     1921 ctttttggat cagagagaca accgccaact gttggcacag tacgctgccg gtcaaacggt
     1981 gctcaatgcc ttcagctaca cgggcgggtt ttcggtgtat gcactggctg ccggcgcccg
     2041 gctcgtgcac tcggtggaca tcagcggaaa agcagtagag gccgccgttc gcaatgccgc
     2101 gctgaccggc gtgcctgccg atcgccatga gggcatcgtg tcggatgtgc tcaaatattt
     2161 gcaaacttcc gaagacggct tgtacgacat catggtggtg gacccgccgg ctttcgccaa
     2221 gagcaccgag aaacgccaca acgccataca agcctacaaa cgcctcaatg cgctggccat
     2281 gcgcaaaatc aaaccgggcg gactgctgtt cacattctcc tgttcgcagg tggtggaccg
     2341 gacgatgttt tataacacca tggtggccgc ggccatagaa gccgggcggg cggtgcgggt
     2401 attgcatcat cttcggcaac cggcggatca cccggtgagc ttgttccatc cggagggaga
     2461 gtatttgaag ggccttgtat tggaggtaga atgaggtagt taagagctgt tggctttggc
     2521 tgagcaatgg caggccaatg ccaacgttcc gacaaggcat ttccagaaaa caaaagaaca
     2581 attcaattgc attgctatga aaaaaatgat gattgctacg ctggcgctga tgagcgttgc
     2641 cttcaccaca ttggaagcgc aaaaaaaaac gcccggccag gaggcggttt cttcggatgt
     2701 tgtagaaagc ctgcctcgtt tccctggatg cgaagaccaa gcagatgaaa aaatcaaaat
     2761 agcctgctcc aacgggaaat tggctgaatt tatagctcaa aacatcgcct atccgcccaa
     2821 agcatttaaa aaaggcattc aaggcaaacc ggtagtttca tttacggtgg aaaaagacgg
     2881 cagcctcagc aatatcaagg tattgaggga aataggaggc ggctgcggca aggaagcagc
     2941 acgtgtaatc cagttgatga acgaccgagg cttgcgatgg attccgggca tgcaaaatgg
     3001 tacaccggtg cgcgtcacat actcgctgcc gattggattc aaattgcaat aaatatcaat
     3061 agaacccacg agacgaaaac aggtaattta attcatacac catcctgcaa tgagcaccca
     3121 caccgccacc ctcaacaacc cgcgcaccat caatgcctgg gcgtttttcg actgggccaa
     3181 ttcagccttc gctctcgtta ttacatcggc tatatttccc gcttatttcg ctgccgtgac
     3241 caacgatcag gtgcgcattt tcggcatcga gatgtccaac agttcgctgt atgcgtatgc
     3301 ggtatcggcc tcgtatctgg tcattgccat ctgctcgccc attttgtcgg gcatagccta
     3361 ttatggcgtc cggcgtatgt ttttcctcaa atttttcacc atcttcggag ccttgggctg
     3421 cctgtcgctg tacttgtttc ggggcatgcc ttctttagcc atcggcctga cgggcttcat
     3481 cgtggccatg atcggttttg ccgggggctt ggttttttac aattcctttt tgccgcaaat
     3541 cgtgaccgaa gaccgctacg accgcgtgag cgcgcgcggc ttttccttcg gctatgtggg
     3601 cagcgttttg ttgttgttgg tcaatctttg gatgattctc aattcagaaa aaatcggctt
     3661 tgccaatgag ggcgtggcca cccgcttggc cttcgtcatg gtaggactgt ggtggatcgg
     3721 ctttgcgcaa atccccttcc gccgcctgcc ccgcgacccc aaagaccgcc ccaaacagca
     3781 attgctgacc aaaggcttca aagaattggc caaagtagcc cgcatagtag ctcaatcaaa
     3841 agacatgacg cgctttctcg gctcgttttt ctgttacagc gccggcgtgc aaacggccat
     3901 tttcctggcg gccacctttg ctgaaaagga attgaaattt gagagcaccg aactcatcat
     3961 catcattctc atccttcagt tggtggccat cggcggggcc tatttttttg ccaaagtatc
     4021 cgactggcgg ggcaacaaat tctcactcat ggccatgctc gtcatctgga cggggatatg
     4081 ttttgcagcc aatcaggttt acaccaaaac gcagttttac ttcctggccg ctgcggtggg
     4141 attggtcgta ggcggcatac aatcgctgtc gcgctccacc tattccaaac tcatcccgga
     4201 aggcaccaaa gacactacct cgtttttcag cttttacgat gtgctggaaa aaaccgccat
     4261 catcatcggt acattcggct tcggcttcat cgcacaggtc acgggcagca tgcgcatgag
     4321 cgtgttgttg ctggccgctt ttttcgtgct cggcatggtt attctggctt cgnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnng ccatacgagg gaagtttagt ttaaaggtgt cgaattagaa
     4441 ccctttaaaa aagaagccgg actgaatagt ttttatctta catcgaaaaa tggaaaataa
     4501 aaagcatcag ccaaagacct ccgaaggctt caatgaaatc ttactgtaca ctacgcccaa
     4561 cggcaatgtg aaagtggaaa tttatctgca aaacgaaacc atttggctta ctcagcaaag
     4621 aattgccgaa ctgtttgggg tggacaggag tgtggttacg aaacatttag ctaatatcta
     4681 catagaggga gaattgagta aagaggcaac ttgtgcaaaa attgcacagg ttcaaacgga
     4741 ggggaatcgg gaggtaaacc agctaattga attctacaac ttagacgcca tcatttcagt
     4801 aggctatcgg gtaaacagca gccgggcaac agctttcaga atttgggcta ccgagcggct
     4861 taaagaatac atcatcaagg gctttaccat ggatgatgag cgtttgaaga accccaacaa
     4921 catttttggt aaagactatt ttgaagaaca gcttgcccgc atacgcgaca tacgcagtag
     4981 tgaacgccgg ttttaccaaa aaatcaccga tatctatgcc caatgcagcg ctgattacaa
     5041 agcagatgca gaaattacca aaaccttttt tgccacagtt caaaataaat tacactgggc
     5101 tattacaggg caaacggctg ccgaaattat ccatgcgaga gccgacagca ataaggaaaa
     5161 catggggctg acaaactgga aaaatgctcc tgccggaaaa attcgtaaat cggatatggg
     5221 cattgccaaa aactacctga acgaacagga attagacggg ctgaaccgca tcgtatccat
     5281 gtatctcgac ttgccgaaat gcaagccaac agaggagttg tcatgtacat gaaagactgg
     5341 atagcgcggc tggacgcttt tttacaattt aatgaacagg ccattctgaa agacgccgga
     5401 aaagtaagtc atgaagtagc tttagctctt gcggaaactg aatttgacaa gtttaacaaa
     5461 aagcaagatg ccttgtatga aagcgatttt gataagttga ccaaaacact ttccagcaac
     5521 aaaaaagaaa aatgataaat aacttcatag ctctatgagc aacccaacca ccaccgttcg
     5581 tcccggcgaa gaactgccct tggacgcctt gagtgattac ctgctcgccc attggccggg
     5641 cttttcccaa atcaccgcac tgcggcagtt tcccggcggg tattccaacc tcacctacct
     5701 gttggaaacc gacgccggcg cggtcgtctt gcgcaggccg cccttcggcg cacagatcaa
     5761 atcggcccac gacatggggc gcgaatttcg ggtgctctct ctgctgcaag gccattttga
     5821 caaaataccg cagcccgtgc tcatctgcga agacgatagc atcatcggag cgccgtttta
     5881 cctcatggag caggtgcagg gcgtcatttt gcggtccaaa ccgccgaagg aatttcaact
     5941 cagtcccgac aacatgcgcc gcatatcgga agcctccgtg gacacgctgg ccgccctgca
     6001 cgccattgat ctggaggcga gcggactgct ccaattgggc aagccgcagg gctatatcga
     6061 acgccaaacc accggctggg tggagcgtta ccgcaaagcc gagaccgatg ccatagccga
     6121 catggacgcc gtggcggagt ggctgactgc gcatcgcccg aacgacggag cgcccgcttt
     6181 tttgcacaac gatttcaaat acgacaacat cgtactcgat cccgccgatc cagggcgcgt
     6241 agtggcggta ttggactggg aaatggccac cgtgggcgat ccgctgatgg acctcggcac
     6301 taccctcgcc tactggaccg aagccgccga ggccgaagcg ctgcccttag ctgccgccaa
     6361 cctgacctgg ctgccgggca acctccgccg cgagcaggca ttggagcgct acgccgccca
     6421 aaccggccga aatgcggatg attttctgtt cttcttcgtg ttcggcgctt tcaaaatcgg
     6481 agtcattgtg cagcagattt acgcccgcta caaaaaaggc tatacgcagg atgcccgctt
     6541 tgccggcctg ctggatgcgg tgcggcattt cggcagaatg ggcgccgggg ccatcgagcg
     6601 gggtcggatg ggcgggtaag agtcggcgcg acgaacagaa tggcggcgtt tagcgcgcga
     6661 ccgttatgtg tctttttgcg gggcgttttc ttttccttcg attctttctt tgaattcttg
     6721 tgccgtcaat acatccacct tgggaaaagg aatttttaga aggacgttga agtgcttgtc
     6781 atcgctcacc aaatattttg catcacaggc aattgcacaa tccacaaatt tgttgtcatc
     6841 agcatctgtc tcaattagat tccacttgta gtattttgtt atccactgca cattcggggc
     6901 attttctatc aattgcaaaa cgtattctgc aaatctatcc cccatatggt ctttgataat
     6961 ctcctcatat tccaataaaa tatctgtcgt cacacataac tcaaattcct cattgataaa
     7021 agcttcgagt atgggccggt atcttgactt acttgaaatg gaaacaagca agacattcgt
     7081 atcaagcact attttcaaaa tggtcagttg ttagggttgt aaggagtcct cttgtgggtt
     7141 ttcaagatcc tttccatgtc ctcatcggtc caattgtttt tctcccaaat atcatcggcc
     7201 attttagtta ttttttggtc taaatatttg actatcagtc tcttgatttc aatcaggtca
     7261 ttgtcatcca attctctcga aaacagcttc agtatttcta attgatgctt gttgaggcta
     7321 ttaagttgtg gtacagcgtt cattactcat ctgttttgtc atgcaaatct attaattttt
     7381 caacttcttt gcaagatact atttttgccc gctccttatg ggatcttttt ctgtaagggg
     7441 cgataaaaaa ataaagtgat cattttcgcc aaaggattag ataagtaaac gtttcatttt
     7501 aagtgtctta tctaatcctt tggcctaaag aaaatgaaca ctttgttttt gccccactac
     7561 tatgactgcc cattgcgttt ccagctcaac ctcgacacca ttttgggagc cggctcccga
     7621 cactgacggt acacaatttg acaaatcttg aagatttgtc aaattttcac gccgcgtcaa
     7681 aatcagtcgc ggttcacaga tttccatttt tggaattgct acacaggctt tgccctgttc
     7741 aatttgtgct acatacgatg ggctgcggaa catcttcctc tccggcggtg tttgctatct
     7801 ttgcaaaaaa ataagaaatg tttcccgaat acgatgtcat tgtggtaggc gcgggccatg
     7861 ccggttgtga agcggcggta gcggcggcca atctcggctc caaagtgctg ttggccacca
     7921 tgaatatgca aaccattgcc caaatgtcgt gcaacccggc catgggcggc gtggccaaag
     7981 gccagattgt gcgcgaagtg gacgctctgg gcggctactc cggcattgtg accgacgaaa
     8041 cgatggtgca gttccgcatg ctcaaccgct ccaaaggccc ggccatgtgg agtccgcgcg
     8101 cgcagagcga ccgcatgctc tttgcccgca agtggcgcca aatgctggaa gcccacccga
     8161 acattgactt ctggcaagac atggtcaacg gcctcgtcgt caaagacggg cgcgtatgcg
     8221 gcgtgcgcac cggcatgggc ctcgaaattc cctgcaaagc cgtcgtgctc accaacggca
     8281 ccttcctcaa cggcatcatc cacatcggcg aaaagcaatt gggcggcggt cgggccggag
     8341 aaagcgcagc ccgcggcatc accgaacaat tggtgagcct cggcttcgaa tcggggcgca
     8401 tgaaaaccgg cactccaccc cgattggacg gtcgcagcct cgactacagc cgcatggaag
     8461 tgcagcccgg cgacgaacag cccggcaggt tttcttacac cgaaaccacg cccctgacag
     8521 cgcaaatgcc ctgccacatc acctacacca acgacaaggt acacgagatt ttgcgcaccg
     8581 gtttcgacca atcgcccatg ttcacgggcc gcattcaggg gctggggcca agatattgcc
     8641 cgtccatcga agacaaaatt gaccgctttg ccgacaaaga ccggcaccag ctttttgtgg
     8701 aacctgaagg ccgcgacacc tgcgaaatct acgtcaacgg attctcctcc tcgctgccgg
     8761 aagacgtgca attcaaggcg ctccggctca ttccgggttt tgaaaacgcg cgcatgttcc
     8821 gccccggcta tgccattgag tacgactact tcccgcctac gcagttgaaa ctgacgctgg
     8881 aaacgcattt ggtcaaaaac ctcttttttg cgggccagat caacggcacc accggctacg
     8941 aagaggccgc ctgccagggc ttgatggccg gcatcaacgc gcatctcaac atcgcagagc
     9001 aggagccttt catcctcaag cgttcggagg cttacatcgg cgtgctgata gacgacctca
     9061 tcaacaaagg cacgcaggag ccgtaccgca tgttcacctc ccgcgccgaa taccgcatcc
     9121 tcttgcgcca ggacaatgcc gacctgcgcc tcacgcccct cgcctaccag atgggcatga
     9181 tgaacatgga ggcccgcatg gagcgcgtgc gcgccaaaga ggaagcggcc aaagccattg
     9241 agcgcttcct gcgcgatacc agcgccgatc ctgccgaatt gaacggcttt ttgtcggaat
     9301 tggacagctc gccgctgcgc caaaaaacca agctgcacgg catcctgctg cgccccaacg
     9361 tgagcatggc cggactgagc cgggcagtac ccgctttgca ccacttcctc gcaggtttcg
     9421 atcaggaatc catcgaattg gccgagatca atctgaaata cgagggctac attgacaagg
     9481 agcgcgaaat ggccgacaag ctcaaccgtt tagaggaggt aaaactgccc gaaaacttcg
     9541 attatcaacc cttggtatcg ctcagcaaag aagcccggga aaaactctcg cgcatccgcc
     9601 cgcgcaccat cgggcaagcc ggccgcatca gcggggtcac gcaggccgat gtgtcnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     9721 cttgcgtata tatccgatta caaacggctg taaacggcta caaacggcat taaacgttta
     9781 ttcaacggat aagacggcgc ccggccccca cctttgccga aaatcctgtc atggaaatct
     9841 ccgtttcttt gtgctgcaaa gacgaaaagc aatacatcgg cgtcaacacc gtcgttttcg
     9901 atgccgtctt ccccgcccgc atccggcaaa tacagggcgc atactggtcg ccggcgctga
     9961 aatattggct gctgccttat acatcggagc attggaaccg gctcaagtac gttttcaaag
    10021 ataccgactt catttttcaa gctgatttcg agccgccgca agattcaact cttgccgcca
    10081 ccttgccgct accacctgaa gtcgttatag aaatactgct acatccctct tctccgaatc
    10141 tgttgcgtat tttgctgcct aaagtcttgg tcccgcgctg gcttgcttcg gtcaggggca
    10201 taccgggccg caaatggaac cccgaaactg cttgttggga agtgtcatat acccgcactt
    10261 cagtggcata cattcgccaa tatctgcccc cctcgctcgt gcattggcat ttcaccccat
    10321 cggaagacct tcccgacaca ccggcgccgc ctgttcgtcg cacgcctttg ccccctgcct
    10381 catcacaagt tgtacgtccc cgatatgaag cagctgcaac agcgttggag caaaccctgc
    10441 tactgaaacg atacagctac aataccatta aaacatacat cctgtatcaa atcaagacca
    10501 aaaacatttc ggaatcgcat caaaatatga tactgagcgc cctgaagatg ttttatgcag
    10561 aagtattggg gcagcatgaa aaagtgagag atatgatacg acccaaacgt ccgcagaaac
    10621 tccctcacac gctgacagaa aatgaagtat ccgcgctgct gagagctgcc gctaacccca
    10681 agcataaatg cattctgatg ctgatttatt cagcaggtct gcgcctggga gaactgatcc
    10741 gcctgcgctt gaaagatatg gagccggcgg ccggccgtgt ctttgtttat ggcggcaaag
    10801 gcaaaaaaga ccgttgcacc atcctatcgc ctaaggtatg ggagcaatta cagacataca
    10861 tagcagtata tcggcccgta gaatgggttt ttgaaggcca aacaggcggg caatacagcg
    10921 cccgcagcgt tcaggagcta ttcacacagg ccaagataaa atcgggcatt aacccttatg
    10981 ccaccgtgca taccctgcgc cactctttcg ctacccacct gctggaagcg ggcgtggact
    11041 tacgctatat tcaagacttg ctggggcacg agagcagcaa gacgacggaa atctatacgc
    11101 acattacgcg cgtaggttgg cagcgggtgc aaagcccttt ggacaggctg cacctgtgat
    11161 ttttgatgag cgcctctttt tttctgcccg gcattgcatt ttccaaacaa agccgctatc
    11221 tttgcttcca ttcaacgttt ttttgaatcc ccgtggaaaa gaaaccgagt gcctacagaa
    11281 gccgttgaag agaagggggg taagacaaag cggaaataaa cgcaattggg cctataggag
    11341 cgattgcgtt aatgcaaaag ttc
//
LOCUS       DAORLF010000102        11319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3070_length_11319_cov_2.305487, whole genome shotgun sequence.
ACCESSION   DAORLF010000102 DAORLF010000000
VERSION     DAORLF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_11319_cov_2.305487"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="hisB"
                     /locus_tag="PK198_10075"
     CDS             complement(<1..1085)
                     /gene="hisB"
                     /locus_tag="PK198_10075"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRF39125.1"
                     /translation="MKKVLFIDRDGTLALEPHDYQLDSLEKLEFYPGVFYWLSRICRE
                     LDYELVMVTNQDGLGTDSFPEENFWPAHHKIMQAFANEGIHFSEVCIDRSFPHDKLPT
                     RKPGTAMLTHYMDGSYDLAGSFVIGNRLTDIQLAKNLGCRGIWFNGSPQLGATELPHG
                     AETLQEVIALATTDWADIWRLLHLPARSAQVRRTTNETDISIRLLLDGQGRSQIHTGL
                     GFFDHILEQLARHSGCDLDIEVKGDLHIDEHHTIEDTALALGEAFHSALGSKLGMERY
                     VFCLPMDDCLAQVAIDFGGRPWLVWEASFQREKIGDVPTEMFFHFFKSFSDAARCNLF
                     ISVQGDNEHHKIEAIFKALARSVKMALR"
     gene            complement(1082..>1615)
                     /locus_tag="PK198_10080"
     CDS             complement(1082..>1615)
                     /locus_tag="PK198_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39126.1"
                     /translation="GIVVVDEAYSDFSRQESILSRIKQHENLVVLQTFSKACGMAGIR
                     LGMAFAQAPMIGWFNKIKPPYNINILTQQAAMNALRQPEIWEEQLSTILRERDHLIHA
                     LSQMPITEHIFPTAANFVLVRVGDPDGLYRFLVQTGIIVRNRSSVILCEGCLRITIGT
                     PDENSQLIQSLQQYAAL"
     assembly_gap    1616..1662
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1663..2124)
                     /locus_tag="PK198_10085"
     CDS             complement(<1663..2124)
                     /locus_tag="PK198_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39127.1"
                     /translation="MSDFLTRLLRPNIARLRPYSSARSEFKGQASIFLDANENPFDNG
                     LNRYPDPLQWPLKQQIATWKDVLPEQIFLGNGSDEAIDLLVRMFCNPGQDRIITLPPT
                     YGMYEVSADIAGVEVLHIPLSRDFQPDVDAILNAADERTKLLFLCSPNNPTG"
     gene            complement(2230..2673)
                     /locus_tag="PK198_10090"
     CDS             complement(2230..2673)
                     /locus_tag="PK198_10090"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRF39128.1"
                     /translation="MLSVYSDDYFMQQAIKEAETALLKGEIPVGAVVVCDNKIIARGH
                     NQTEQLHDVTAHAEMIAVTAAANNMGAKYLPDCTLYVTLEPCAMCAGALFWSQIGRVV
                     YGAADDKRGFMRFGKALLHPKTTVEYGVREETCAELLTRFFKAKR"
     gene            2769..3392
                     /locus_tag="PK198_10095"
     CDS             2769..3392
                     /locus_tag="PK198_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRF39129.1"
                     /translation="MKIICIGRNYVEHAKELNNPIPERPVVFMKPPSALLVNDKPFYY
                     PEFTKDLHYEAEVVLRICKNGRHVQPEFASEYYDQIAFGIDFTARDLQDELKKKGHPW
                     EIAKGFDGSAVISSFFSIETAQREKGVEFKLRKNGEVVQHGNTSDTLFSFDSIIVHVS
                     KFFKLQVGDYIYTGTPAGVGPVQIGDKLEGFLFLQERFEHVLTCDVR"
     assembly_gap    3563..3671
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3672..4378)
                     /locus_tag="PK198_10100"
     CDS             complement(<3672..4378)
                     /locus_tag="PK198_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF39130.1"
                     /translation="MEVNSMQLGLAFLGMAAVVVGLILYFRNSLAQKSARGMGDKYKS
                     SMNAADLEVRNKYPEADVFRLSPTFFNLGLALSVGITVLALGWTQYEKTVDVSSYMDT
                     LEEEIEVEPPRTAEPPPPPPPPPPPVIQEVPNETVLEDDTPFTDPDIEEDNAPPPPKA
                     DENKPTPPPPPPPPPPAEDNDVEIFKVVEEMPRFPGCENEATTEAKKACADKKMLEFI
                     YKNIKYPAIARENGVEGT"
     gene            complement(4469..4861)
                     /gene="gcvH"
                     /locus_tag="PK198_10105"
     CDS             complement(4469..4861)
                     /gene="gcvH"
                     /locus_tag="PK198_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRF39131.1"
                     /translation="MVFPADLKYTEDHEWLRLSDGNTAYIGITDFAQRELDELVYIEV
                     DSVGESLNKGDVFGTVEAVKTTSELFMPVSGTILEFNPALDEKQGDDPTTVNRDPYGE
                     GWIVKIELSDPSEVEALMDAAAYEATVG"
     gene            5150..>7620
                     /locus_tag="PK198_10110"
     CDS             5150..>7620
                     /locus_tag="PK198_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39132.1"
                     /translation="MNFIIFSSSAMLRSAALFVFFCIMFLATAGAQTLLSGRVTDAAG
                     QALPGANVLVLERSLGDAADAAGNYRIAGLEPGTLRLRVSFVGYETLERKVVVAAGTP
                     ELRLDFRLQELSLSMEPLIVRATRATERTPMTFTELDKTAIARNNTGLDAPYLFRWTP
                     SAVETSDAGAGVGYTGIRIRGSDATRINVTINGIPLNDSESQSVYWVNMPDFLSSVEN
                     VQIQRGVGASTNGPGAFGASINLQTKPLSNEAFADLSLGAGSFNTWRRNVRFGSGRLG
                     NGFSIEGRYSHISSDGYVDRAASDLRSGFLTAGWTGDRSLLKFNVITGQERTYQAWYG
                     IPKDYLKDPVLRRFNPAGTERPGEPYENETDNYRQTHYHLIYNTQWHPRWNLNLALHY
                     TRGAGYYEQYKAQQAFAKYGLDNVTTDNDTIRRTDLVRQLWLDNHFYGGVYSLIWRSE
                     DERTEVTTGGAYSIYDGLHFGEIVWAQFAGSMTGRHRYYENEARKTDFNIYQKWNRRI
                     GSRWDAYLDVQFRRVGYDFLGYNRNLEQVGQNVALSFFNPKIGAQYQVNARSQAYASL
                     AVGSREPNRNDYTENTPDTRPLPERLYNVETGYRHSGQRGALSINGYYMFYHNQLVLN
                     GQINDVGEYMRVNVARSYRLGLEMMAEYRPVKSLRLAANYTLSRNRVAAFTEYVDEYD
                     ADFNWIGQLAVERRQTHLAFSPSHIANAEMEWNLWTDASEQRRLSATLMNKYVGRQFI
                     DNSSDVANALPAYFYSDLRLEFQVRPSFARNLGLSLLVQNLWNAQFASNAWSYRYRAE
                     GALSLDQGFFPQAGRNVLLGLTLGF"
     assembly_gap    7621..7709
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7710..8098)
                     /locus_tag="PK198_10115"
     CDS             complement(<7710..8098)
                     /locus_tag="PK198_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF39133.1"
                     /translation="MTKRSVVAVKGFPIGQVRHIGLDEKGLFIIRLELDKEPVIPADS
                     KFRIEQQSFLGASGIAVDLGIQSTPLSDGDTILLVPPPKSSIADSLSNTLLNIFESLT
                     GSKQRDSILMELRRLNENLEDLKKGQQ"
     gene            8479..>8846
                     /locus_tag="PK198_10120"
     CDS             8479..>8846
                     /locus_tag="PK198_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39134.1"
                     /translation="MKTAIQLLSAMCLLLWAPFASAQMSMGAKRGDYTFGINAGYAFQ
                     SSDVRSAGNGGGFGLTAGRTLLAGAYSPISVDLRGRLLYARTFGLDPLRSFQIQNNKA
                     LNGTQGLNYLSYPANLDEPQG"
     assembly_gap    8847..8879
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8880..>11319
                     /locus_tag="PK198_10125"
     CDS             <8880..>11319
                     /locus_tag="PK198_10125"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF39135.1"
                     /translation="QNYRTTIGEMSAELVFTLEELRRKTGIIASVYGGIGLDGFHSKI
                     DQADAQGNEYFSAYANLNDNRPIAAILRDLKGSVLDGTYETNADGFDDLPKFRAMPSL
                     GVELGFHLTPSLSLHAGHRLTFSGTDLLDGHQWSSSDKDMYHYTNFGITYRFRPPAKP
                     KLLPPVIDITSPSTRTHYTNDPNGLVRARITNVRSAGDVYCRVNGRETPFSFSGEQFT
                     LNFRLQEGRNEVVITAKNTAGSTNADVLIILEGVVLPPPPPPPVGYAPRVSISNPSGG
                     QATVSEAVFAFRATVSDVLSKNDIELTVDGVRRDFTYDSRTGAVSTQLNLREGVNRVR
                     VSARNNFGSDAADAEIRYEPRRFQPVVRITSPENRSETTVNSATVQAEVRYVEARDGI
                     SLYVNGRSISTFEFDAARETFRATVPLNEGSNTIELTARNSTGEARDGISVTYRRPVV
                     QSNPPTVRITSPASGSSTTEVTANVEATVREVTRREDVSFFVNGARSDNFTFNAGTGR
                     FSATVNLVVGNNDIAIRVANTDGSDQAAVSIRRTEKPIEAPQPPRVRITTPANQAVVA
                     RNTVEVRATIDNITDRNAISLLVNGSRFANFNFSTNTRELIATVTLIEGNNSIRVEAT
                     NRDGNANDEVNVRFQKAVPPVVTITAPDDNSTVSNPGATLRARLQNVNDRRQITVSNN
                     GATLSNFNYNTSTGELSAEVTLTEGNNTLRVRAATNDGQDEKSVNVTYRKVAPPTVNI
                     TAPDNNSTTTSATTTLRARVQNANNRNQITVSHNGNTLSNYTFNTSTGELSAEVTLTE
                     GNNTLRVRAATNDGQ"
BASE COUNT         2399 a         3037 c         3116 g         2489 t
ORIGIN      
        1 cgcagcgcca tttttacact ccgggccagc gctttgaaga tggcctctat tttatggtgt
       61 tcgttgtcgc cctgaacgct gatgaagagg ttgcagcggg ccgcatcact gaaggatttg
      121 aaaaaatgaa aaaacatctc ggtgggcaca tctcctattt tttcgcgttg gaacgaggcc
      181 tcccaaacca accagggccg cccgccgaaa tcaatggcca cctgcgccag acaatcgtcc
      241 atcggcaggc aaaagacata gcgctccatg cccaatttgc tgccgagtgc cgagtgaaaa
      301 gcctcgccga gcgccagggc cgtatcttcg atggtatgat gttcgtcaat gtggaggtcg
      361 ccttttacct ctatgtccag atcgcagccg gaatggcggg ccaattgctc gagtatatga
      421 tcgaaaaagc ccaggccggt gtgaatttgg ctgcggcctt gcccgtcgag cagcaggcgg
      481 atggaaatat cggtttcgtt ggtggtgcgg cgcacctgag cgcttcgggc cggcaggtgc
      541 aataagcgcc agatgtcggc ccagtcggta gtagccaatg caatgacctc ttgcagggtt
      601 tcggcgccgt ggggtagttc ggtagcgccg agttgtgggg atccgttgaa ccagatgccc
      661 cggcagccga ggtttttggc caattgtatg tcggtgaggc ggttgccgat gacgaaagag
      721 ccggccagat catagctgcc gtccatgtag tgagtcagca tggcggtacc cggtttgcgg
      781 gtgggcagct tgtcgtgggg gaagctgcgg tctatgcaca cctcgctgaa gtggatgcct
      841 tcattggcga aagcctgcat gattttgtgg tgtgccggcc agaaattttc ttccggaaag
      901 gaatctgtac cgagtccgtc ctgattggtg accatgacca attcgtagtc caattcccgg
      961 caaatgcggg agagccagta aaaaacgccg ggatagaatt ctaatttctc taagctatcc
     1021 aattgataat catgcggttc gagggccaat gtgccgtcgc gatcaatgaa gagtactttt
     1081 ttcacagtgc agcgtattgt tggagtgatt gtatgagttg gctgttttcg tcgggtgtgc
     1141 cgatggtgat gcgcaggcag ccttcgcaga ggatgacgct ggagcggttg cgcacgatga
     1201 tgccggtttg caccaaaaac cgatacagtc cgtcggggtc gccgacgcgg acgagcacaa
     1261 aattggcagc cgtgggaaaa atatgttcgg tgatgggcat ttgggaaagc gcgtgtatga
     1321 gatgatcgcg ctcccggaga atggtgctca gttgctcctc ccagatttcg ggttgccgga
     1381 gggcgttcat ggcagcctgc tgggtgagga tgttgatgtt gtagggcggt tttattttgt
     1441 tgaaccaccc gatcatgggc gcttgggcga aagccatgcc gagacggata ccggccatgc
     1501 cgcaggcttt ggagaaggtt tggagtacga cgaggttttc atgttgcttg atgcggctca
     1561 agatgctttc ttgccgggag aagtcggagt aggcttcgtc caccaccacg atgccnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccggtgg gattgttggg
     1681 gctgcacaaa aagagcagtt tggtgcgctc gtcggcggca ttgaggatgg cgtccacgtc
     1741 gggttggaaa tcccgcgaaa gcggaatgtg cagtacttct acgcctgcaa tgtcggcgct
     1801 cacctcgtac atgccgtagg tgggcggcag ggtgatgatg cggtcctggc cgggattgca
     1861 aaacatgcgc actaagaggt caatggcttc gtcgctgccg ttgccgagaa aaatctgttc
     1921 aggtagtaca tctttccatg ttgcaatttg ctgtttgaga ggccattgca gtgggtcggg
     1981 gtagcgattg aggccgttgt cgaaggggtt ttcattggca tccagaaaga tggaagcctg
     2041 tcccttgaac tcgctgcggg cggaggagta gggccgcagt cgggcgatgt tggggcggag
     2101 gaggcgggtg aggaagtcgg acattctttt tgagttttat gggcgggtag aacccgcggg
     2161 atagcccgca cggtggaacc tcgcatgggc gaaaggtaga acctcgcacg ggcgagcagg
     2221 ttcatgttct tatcgtttgg ctttaaaaaa acgcgtgagc aattccgcgc aggtctcctc
     2281 ccggacgccg tattccaccg tcgttttggg atgcagcagc gccttcccga aacgcatgaa
     2341 gccccgtttg tcgtcggcag cgccatatac caccctgccg atttgcgacc aaaacaaagc
     2401 gccggcgcac atcgcgcaag gctccagggt gacgtagagc gtgcagtccg gcaggtactt
     2461 ggcgcccata ttgttggcgg cggcggtgac ggcaatcatc tcggcatggg cggtgacgtc
     2521 gtgtagttgc tcggtttggt tgtggccccg cgctatgatt ttgttgtcgc acaccacgac
     2581 ggcgcctacc ggaatttcgc ctttcagcag ggcggtttcg gcctctttga tggcctgctg
     2641 catgaagtag tcgtcgctgt aaacggaaag cataatgccg taaattgatg tgttttgaaa
     2701 cggcccgaat cgcttcggaa ccgcgcaaaa gtactacatt tgcaggcgta ccgtaaaatt
     2761 tcactgttat gaaaatcatt tgcatcggcc gcaattatgt ggagcacgcc aaagaattga
     2821 acaaccccat accggagcgc ccggtggttt ttatgaagcc gccctctgcg ctgttggtca
     2881 acgataagcc tttttattac ccggaattca ccaaagacct ccactacgag gccgaggtgg
     2941 tgttgcggat ttgtaaaaac ggacggcacg tgcagccgga gtttgcttcc gagtattacg
     3001 atcagattgc tttcggcatt gatttcacag cccgcgattt gcaggacgaa ctgaaaaaga
     3061 aaggccaccc ctgggaaatt gccaaaggat ttgacggctc ggcggtcatc agttcgtttt
     3121 tttcgataga aacagctcag cgcgaaaagg gcgtagagtt caaactgcgc aaaaacggcg
     3181 aggtggtgca gcacggcaac acttccgaca ccttgttttc tttcgattcc atcatcgtgc
     3241 atgtctcgaa gtttttcaaa cttcaggtgg gcgattatat ttatacgggt actcctgcgg
     3301 gcgttgggcc ggtgcagata ggcgataaat tggagggttt cctgttttta caggagcgct
     3361 tcgagcatgt gctgacttgc gatgtgagat agagctataa agcaccacag cccccggaaa
     3421 ctgagtcccg gaggctgtgg aaaattttat tttttagaac agaaaacgat cactccaatt
     3481 tgaagcgcac cggcaggttg aactgcacgc gtacggagcg gccccgctgt ttgccgggag
     3541 tccacttaat gttgctttca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn ngtaccttct acgccgtttt cgcgggcgat ggccggatac ttgatgttct
     3721 tgtagatgaa ctcaagcatc tttttgtcgg cgcaggcctt tttggcttcg gtagtagctt
     3781 cattttcgca gccggggaag cgcggcattt cttctaccac tttgaagatt tccacgtcat
     3841 tgtcttcagc cggcggcggc ggtggcggtg gtggtggtgg cgtgggcttg ttttcatctg
     3901 ctttaggcgg tggtggagca ttatcctctt caatatccgg atccgtaaac ggtgtgtcat
     3961 cttccagtac cgtttcgttg ggtacttcct gaatcaccgg cggtggcggc ggcggtgggg
     4021 gtggcggcgg ctcggcggta cgcggcggct ctacctcgat ttcttcttcg agggtatcca
     4081 tataagagga tacgtccacg gtcttttcgt attgtgtcca accgagtgcc agcacggtaa
     4141 taccgacgga gagcgccagg ccgaggttga agaacgtcgg gctgagacgg aacacatccg
     4201 cttcggggta cttgttgcgc acttcgagat cggcggcatt catcgaagac ttatacttgt
     4261 ctcccattcc ccgagctgat ttctgagcta aagaattgcg gaagtaaaga ataagtccaa
     4321 caacgacggc agccataccg aggaacgcca agcctaattg catgctgttg acttccatac
     4381 ttttgactgt ttattttgtg agaaaaagta cttacaacgg gtaagatgag gctgcattcg
     4441 tttttggcgt atgctggccc aatttttttt atccaacggt agcttcgtag gcggcggcgt
     4501 ccatcagtgc ctccacttcg gaaggatcgc tgagttcgat ttttacaatc cagccttcgc
     4561 catacgggtc gcggttcacg gtggtggggt cgtcgccctg cttctcgtcc aaagccggat
     4621 tgaattccag aattgttccg gaaacgggca tgaacagttc ggaggtggtt tttacagcct
     4681 ctacggtgcc gaaaacatcg cccttgttga ggctttcgcc aacggaatcc acctcgatat
     4741 acaccaactc gtccaattcg cgctgagcaa aatcagtaat gccgatgtat gcggtgttgc
     4801 cgtcggagag ccggagccat tcgtgatctt cggtgtattt taaatctgcg ggaaatacca
     4861 tgatttttct tcgttttgta aagcagttcg gaaacggctc caaaaatagg gatgtttgta
     4921 aaacctgcaa gcacgcggcc tctttttttt ctatctttgc cgctcaatct cttcaggggt
     4981 gccgcgctat gggtgcggct gagatcatac cctttgtttc gcttcgcagg cgagacagga
     5041 acctgcccgg gtaatgccgg gaagggaaac tgcgcgacag ggggtcgttg caacgcgccg
     5101 ttttgcccct gaaacggtgg attctatcac cgttttgctt tgtttgcaca tgaattttat
     5161 cattttttct tcctccgcga tgctgcgcag cgcagcattg ttcgtcttct tttgtatcat
     5221 gtttttggcc acagccggcg ctcaaaccct gctcagtggc cgggtgaccg atgccgccgg
     5281 acaagccttg ccgggcgcca atgtgctggt attggagcgc tcgctgggcg atgccgccga
     5341 tgccgccgga aattatcgca ttgccggact ggaacccggc acattgcggc tgcgcgtttc
     5401 ttttgtgggt tatgaaacgc tggagcgcaa ggtggtcgta gcggcgggta ctcccgaact
     5461 gcgcctcgat tttcgcctgc aagaactcag cctgtctatg gagccgctga ttgtgcgggc
     5521 cacccgagcc accgaacgca cgcccatgac ttttacggaa ttggacaaaa ccgccatcgc
     5581 gcgaaacaac accggcttgg atgcgcccta ccttttccgc tggacgccct ctgccgtaga
     5641 aacctccgat gccggcgcag gcgtgggtta cacgggcatt cgcatcagag gcagcgacgc
     5701 cacccgcatc aacgtgacca tcaacggtat tccgctcaat gactcggaat cgcaaagtgt
     5761 gtattgggtg aacatgcccg atttcctcag ttccgtagaa aacgtgcaga ttcagcgcgg
     5821 cgtgggcgct tctaccaacg ggccgggcgc gttcggggcc agcatcaacc tgcaaaccaa
     5881 gcccttatcc aatgaagcct ttgcagacct gagcctcggc gcgggttcgt tcaacacctg
     5941 gcggcgcaat gtccggttcg gcagcggcag attgggcaac ggtttcagca tcgaggggcg
     6001 gtattcgcac atcagctccg acgggtatgt ggaccgcgcg gcctccgatt tgcgctccgg
     6061 gttcctcact gccggctgga cgggcgaccg cagcttgctc aaattcaacg tcatcaccgg
     6121 gcaggagcgc acctatcagg cttggtatgg cattcccaaa gactacctca aagacccggt
     6181 gctgcgccga ttcaaccccg ccggaaccga acgccccggc gagccttacg aaaatgaaac
     6241 cgataactac cgccaaacgc attaccacct gatttacaac actcaatggc atccgcgctg
     6301 gaatctcaac ctcgctttgc actacacgcg cggcgccggt tattacgagc agtataaggc
     6361 gcagcaggct ttcgccaaat acggcctcga caacgtgact acggataatg acaccattcg
     6421 tcgtaccgac cttgtgcgcc aactctggtt ggacaatcat ttttacggcg gcgtttacag
     6481 cctcatctgg cgcagcgaag acgaacgcac cgaggtgacg acgggcggcg cttacagcat
     6541 ttacgatggt ttgcacttcg gcgagatcgt gtgggcgcag tttgccggct ccatgacagg
     6601 ccgtcaccgc tattacgaaa acgaggcccg gaaaacggat ttcaatattt atcagaaatg
     6661 gaatcgccgc atcgggtcgc gctgggatgc ttatttggat gtgcagttcc gccgggtggg
     6721 ttatgatttt ctggggtaca acagaaactt ggagcaggtg ggccaaaatg tggcgctttc
     6781 gttttttaat cccaaaatcg gcgcgcaata ccaggtcaat gcccgcagcc aagcctacgc
     6841 ctccctcgcc gtgggcagcc gcgaacccaa tcgcaacgat tacaccgaaa atacgcccga
     6901 tactcgcccg ctgcccgaac gcctctacaa tgtagaaacc ggctaccggc acagcggtca
     6961 acgcggcgcg ctgagcatca acgggtacta tatgttttat cacaatcagt tggtgctcaa
     7021 tggtcaaatt aatgacgtgg gcgaatacat gcgcgtgaat gtggcccgta gctaccgtct
     7081 gggattggaa atgatggccg agtatcgccc tgtaaaatcg ctgcggttgg ccgccaatta
     7141 cacactgagc cgcaaccggg tggctgcctt caccgagtat gtggacgaat acgatgcaga
     7201 tttcaactgg atcgggcagc ttgccgtgga gcgccgccaa acacacctag ctttttcgcc
     7261 ttcgcacatt gccaacgcag aaatggagtg gaatctgtgg acagacgcct ccgagcagcg
     7321 ccgactgagc gccacgctga tgaataaata tgtgggccgt cagtttatag acaactcctc
     7381 ggatgtggcc aatgcgctgc cggcttattt ttacagcgat ctgaggttgg aatttcaggt
     7441 gcggccttcg tttgcgcgga atctggggct gagtttgttg gtgcagaacc tgtggaatgc
     7501 gcagtttgcc tccaatgcct ggtcgtaccg gtatcgggcc gagggcgcgc tgtcgctcga
     7561 tcagggtttc tttccgcagg cgggccggaa cgtgctgctg gggctgacgc tggggttttg
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc attgctgccc ttttttcaaa tcttccagat
     7741 tttcattcag tcgtcggagt tccatcagga tagaatcgcg ctgtttcgag ccagtcagcg
     7801 attcaaaaat gttcagcaat gtgttggaga gcgagtcggc gatggaggat tttggcgggg
     7861 gtactaataa tatggtgtcg ccatcagata ggggcgtact ttggatgccg aggtcaacgg
     7921 caatgccgga agcgcccaga aagctctgct gttctattcg gaattttgaa tccgccggga
     7981 tgaccggttc tttatccaat tccaaccgga tgatgaacaa ccctttttca tccaaaccaa
     8041 tatgcctcac ctgtccgatg gggaaacctt tgacggccac taccgaacgc ttcgtcatct
     8101 gcctgatctg tgcgcagata gacggtgcga tgctggtctg tgcaggaaaa aagcagcagg
     8161 aagaagagga gtatgggtcg gaattgcatg gcttttggat gagagtcggg gtcaaaaata
     8221 aagcgctgtc cgggtttctt gttattttcg ggttaaacat atttcctgcg ggcgaacact
     8281 attaacgccg cctgttcaaa agcgcggcct ttagcaatct tcatcattcg actgaaaagc
     8341 aaatgtttaa cacaaaagcc ttgagaaacc tacatgggcg ctcttgtagt catatctgtt
     8401 aaaactaaaa tatgacagat tttgcactgc tgcgtaaaat caataacttc gtcctgaatt
     8461 tatcacaaca gttaccttat gaaaaccgct attcaactac tcagtgcgat gtgcctgctt
     8521 ttgtgggcgc catttgcatc tgcccaaatg agcatgggcg ccaagcgcgg cgattacacc
     8581 ttcggcatca acgccggtta tgccttccaa tcgagcgacg tgcgcagtgc cggcaacggc
     8641 ggcggcttcg gcctcacggc aggtcgtacc ctgttagcag gggcctactc tccgatttct
     8701 gtggacttgc gcgggcgcct gctctatgcg cgcacgttcg gcttagaccc gctgcgttcc
     8761 tttcagattc agaacaacaa agcgctgaac ggcacgcagg gattgaacta cctgagctac
     8821 ccggccaatt tggacgaacc ccagggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8881 aaaactaccg caccaccatc ggcgagatgt cggctgaact cgtttttacc ctcgaagaac
     8941 tgcgccgcaa aacgggcatc atcgcctctg tgtacggcgg cattggcctc gacggctttc
     9001 acagcaaaat agaccaggcc gacgctcagg gcaacgagta cttcagcgct tatgccaacc
     9061 tgaacgacaa ccgccccatc gccgccatcc tccgcgatct caaaggcagc gtcttggacg
     9121 gcacttatga aaccaacgcc gatgggttcg acgatctccc caaatttcgc gccatgcctt
     9181 cacttggcgt agaactcggc tttcacctca cgccctctct gagtttgcac gccgggcacc
     9241 gcctcacgtt ttccggcacc gatttgttgg acgggcacca gtggtcttcc tccgacaaag
     9301 acatgtacca ctataccaac ttcggcatta cctaccgttt ccgtccgccg gccaagccca
     9361 aactgctgcc gcccgtcatt gacattactt ctccctccac ccgcacgcac tacaccaacg
     9421 accccaacgg cttggtgcgc gcccgcatta ccaatgtgcg cagcgccggc gatgtgtatt
     9481 gtcgcgtgaa cggccgcgag acgccattca gtttcagtgg ggagcagttt accctcaact
     9541 tccggttgca ggaaggccgc aacgaggtgg tcatcaccgc caaaaacacc gccggttcta
     9601 ccaatgccga tgtgctcatt attttggaag gcgtggtatt gccgcctccg cctccgccgc
     9661 cggtgggcta tgcgccgcgc gtgtccatca gcaatccgtc gggcgggcag gctaccgtga
     9721 gcgaagcggt attcgctttc cgcgcaacgg tgagcgacgt attgagcaaa aacgacatcg
     9781 aactgacggt ggacggagtg cgccgcgact tcacttacga ttcccgcacg ggcgccgttt
     9841 ctacccaact caatctgcgc gagggtgtta accgcgttcg ggtgagcgcc cgcaacaact
     9901 tcggctccga tgccgccgat gccgaaattc gctacgaacc gcgtcgtttc cagcccgttg
     9961 tgcgcattac ctcgcccgaa aaccgcagcg aaaccaccgt caactccgcc actgtgcagg
    10021 ccgaagtgcg ttatgtagaa gcccgcgacg gcatcagtct gtacgtcaat ggtcgcagca
    10081 tcagcacttt tgaatttgac gctgccagag aaacattccg ggccaccgtg ccgctcaacg
    10141 aaggctcgaa caccatagaa ctgaccgccc gcaacagcac cggcgaggcc cgcgacggca
    10201 tctccgtcac ttaccgtcgc ccggtggtgc aaagcaatcc gccgaccgtg cgcatcacct
    10261 cgccggcatc gggcagttct accaccgaag ttacggccaa tgttgaggcc accgtgcgcg
    10321 aagtgacgcg ccgcgaggat gtgtccttct ttgtgaacgg cgcccgcagc gacaatttca
    10381 ctttcaatgc aggtaccggc agattcagcg caacggtgaa tttggtggtg ggtaacaacg
    10441 acatcgccat tcgggtagcc aacaccgacg gcagcgatca ggccgccgtg agcattcggc
    10501 gcaccgaaaa accgatagaa gcgccacagc cgccccgagt gcgcatcacc acccctgcca
    10561 atcaggccgt tgtagctcgc aacacggtag aagtgcgcgc taccattgat aatattaccg
    10621 accgcaacgc catcagtctg ttggtcaatg gttcgaggtt tgccaatttc aattttagta
    10681 ccaatacccg cgaactgatt gccaccgtca cactcatcga gggcaacaac agcatccgcg
    10741 tggaagcgac caaccgcgac ggcaatgcca acgacgaggt aaatgtgcgt ttccaaaaag
    10801 ccgtgccgcc ggtcgtgacg atcaccgcgc ccgacgacaa cagcaccgtt agcaaccccg
    10861 gcgcgacatt gcgggccaga ttgcaaaacg tgaacgaccg tcggcagatc accgtgtcga
    10921 acaacggcgc cacactgagc aatttcaact acaacacctc caccggcgaa ctaagcgccg
    10981 aggtaacact cactgaaggc aacaacaccc tgcgcgtgcg ggccgccacc aacgatggtc
    11041 aggatgaaaa atcggtgaac gtgacgtatc gcaaagtcgc gccgcccacc gtgaacatca
    11101 ccgcgccgga caacaacagc accacgacct ccgccaccac cacgctgcgg gccagggtgc
    11161 aaaatgccaa caaccgcaat cagatcaccg tatcgcacaa cgggaatacg ttgagcaact
    11221 acaccttcaa cacctccacc ggcgaactca gcgccgaggt aacgctcact gaaggcaaca
    11281 acaccctgcg cgtgcgggcc gccaccaacg atggtcagg
//
LOCUS       DAORLF010000103        11319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3071_length_11319_cov_2.090199, whole genome shotgun sequence.
ACCESSION   DAORLF010000103 DAORLF010000000
VERSION     DAORLF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3071_length_11319_cov_2.090199"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    284..325
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<326..>1324)
                     /locus_tag="PK198_10130"
     CDS             complement(<326..>1324)
                     /locus_tag="PK198_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39136.1"
                     /translation="AVFADFIANEKPLYCRIEGQTYFPVLRHYLVVAGWARWDGALAG
                     GDWKNLSYERVVFPPIPYSPQALDKKNFNYVNPFGKQRVESWRFRHWMGTDQIGRDVA
                     AGMIWGARTAMLVGLVSMGVALLIGILLGAVAGFFGDRSLKVSIAGVVLNLIVGAAGL
                     FYATVPGPDGAFRGWGQAVLIFATILLIANALYWLLSKIPFLKRRVYLPADMLVLRLI
                     EVMNSIPALLLLLSVAAVLRKPSLAATMLIIGLIRWTGIARFIRAELLRIRSLGYIES
                     ARVLGLSEWRILWRHAAPNALGPVLIALAFGIAGAILLEAFLSFLGIGLSPEEVTW"
     assembly_gap    1325..1453
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1621..2724
                     /locus_tag="PK198_10135"
     CDS             1621..2724
                     /locus_tag="PK198_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF39137.1"
                     /translation="MSNKLRILVAPLDWGIGHATRCVPIIRELLRQGAEVYIASNGRP
                     LKVLQREFPELPTLTLPRYDVKYPFRSIILNMLLQLPRLLWAIFAEHRMVDRWVRRYG
                     FDGIITDNRLGCYSRRVPTVFITHQVHLKAPDENIARFTNRINHSFMRRYGRCWIPDW
                     DGSQSLSGTLSRPSAHPHTVYTGPLTRLRPVQSAIKYDVLVLLSGPEPQRTYVEKAIL
                     KQAAGLPYNMLLVQGKPETDHTIQGLDNVEIVPFLDAPELSRAMAESRMVVCRSGYST
                     VMDLAVLGKRALYIPTPGQPEQEYLAEYLGERGASLWQDQEEIRLAEGIAGVLGLPEP
                     DMSLVSRRLLETAVRDFLPAASPSEDQMSWATA"
     assembly_gap    2744..2832
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3047..4606
                     /locus_tag="PK198_10140"
     CDS             3047..4606
                     /locus_tag="PK198_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRF39138.1"
                     /translation="MSAGLITLIVGWVFVLVWVPFVVLSQRKMHPKELFTLFFAEMWE
                     RFSFYGMRAFLILYMASEIFKQFGQTDADTKSYGVYGAFNALLYAAPIVGGMLADRMI
                     GFRKAVMLGGIFMALGQFTLAANASMGSQSLLFYGGLGLLAVGNGFFKPNISSFLGTF
                     YDRNDNRKDGAFTIFYMGINVGAFLAPITCGYLAREVHYSLGFLAAGVGMLLGLVVFW
                     NNIPKDKGNAPDPVAAAKAVFMGLNRFWVIVAASLAFIPVFALLIGAEKVTNWILIIA
                     GLGVIGYLIFSALSSEDKKEGQRLLVFIVLFFFHMIFWTLFEQAGGSLNILTDRYVNK
                     MGIETSQFQSVNPLFIILLAPVFTWIWTQLGKRKQEPRTPMKFVFALLQMALGYFIIV
                     IGAKSVLGDVDNGGMIPLYFLLFMYLFHTTGELSLSPVGLSVVTKLSPAKTVGFVMGS
                     WFLSIAFAHKIGGYLGQLIATPQEGASKASELTAFINVYMNWGVYLVLGCALLLLLLT
                     PVLKKWMHGVH"
     gene            4901..6862
                     /locus_tag="PK198_10145"
     CDS             4901..6862
                     /locus_tag="PK198_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRF39139.1"
                     /translation="MENQQQDFKPYISAKDFIPEFTLKAILLGSFFGVLFGAATVYLG
                     LKVGLTVSASIPIAVLAISIFKKIGKATILESNIVQTIGSAGESVAAGVVFTLPALLF
                     LTGGRAYFQYFQIFVLALIGGILGVLFMVPLRRSLIVKEHGKLPYPEGTACADVLVAG
                     EKGGKLAQKVYYGLGVAFLYKLLMSIIGLWKDVPTLLFKRGSSLPNGSVSAEITPELL
                     GVGYIIGPRIAGIMAAGGVLSWLVLIPLITIFGDGLAGPLAPEMTQRIADMGPGDIWN
                     RYIRYIGAGAVTFGGLVTLIKTIPTIVSAFRDSFKDLKESRASKGVQRERTERDIPLV
                     YVLVGSAVLLVLMTILPNVPTNFLSAMLIIVFGFFFVTVSSRIVGLIGSSSNPISGMT
                     IATLMATSLIFLGVGWSGDAYQPIALVVGSIVCIAAANAGATSQDLKTGYIVGATPVK
                     QQLGLIIGVLVSVVVIGFTMLLLERTIGIGEATAEHPNPLPAPQATLMATVIKGLLSQ
                     NLPWGLVIAGMGIAAVVELCGVNSLAFAVGAYLPLSTSTPLLAGGFVKWIADKINKSD
                     KEESDIGPGALFSSGLIAGGAIMGIVIAILIGSTYGVDAEGKPRSLLSYLNIGLGESL
                     GSGGDLLGLIAFVLLGAVLLRFATQKEKA"
     gene            6997..9573
                     /locus_tag="PK198_10150"
     CDS             6997..9573
                     /locus_tag="PK198_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRF39140.1"
                     /translation="MKHTFTFLLLWAALAAFAQKQPLTYFLPDIPYDKNLPTPEAYLG
                     FQIGEWHISHDQLLGYMKTLAAASPRAKLVEHGRTFENRPLIHLIITSEKNHKSLDEI
                     KAQHVALSNPSKSAGVDLNKVPAVLYQGYTIHGNEPSGSNAAMLAAYYLLAGKSKDLD
                     RLLEDVIIIFDPCFNPDGMNRFASWVNTHRNKNLSSDPADREFNEPWPRGRSNHYWFD
                     LNRDWLPAQLPESQGRVRIFHEWKPNVLTDHHEMGSNTTFFFMPGVPSRVHPLTPKLN
                     QELTERIGNYHAEALDGIGSLYFTKEGYDDFYYGKGSTYPDANGCVGILFEQASSRGH
                     VQQTDNGDLTFAFTIRNQVATSLSTHKAVREMKKDLLSYQRDFYTSALEDARNDTRKA
                     YIFGDPADRARTAAFIEILRKHQIEVYTLKKDATAAGHQFTAGQAYVVPLDQPQYRLI
                     HGIFETRTAFEDSIFYDISGWTYPLAFNLPYAALEKRAFDDDMIGDKIAGPGIVVETP
                     APEFSEYAYLMEWTEYYAPKALNYLLQQGLRMKVASTGFSMDNRNYPPGTILLPVAGQ
                     SKTPEQIHGLITIASQMGGVRFYDMDTGLTPVGIDLGSDDFKLLKTPEVLLLVGEGVR
                     STDAGEIWHLLDQRYDMRVTKVEMTALGRVDLNKYTAVIMPGGNYGSISGGDRLKEWV
                     QQGGVIVAYQEAVQWLRSNGLAFVEFRQEKASDATPARRPYTKREEDRGALQTSGAIF
                     ENLLDNGHPIGYGYTRDKMPVFRSGNLYFEPAKNPYATPLLYSSNPLMSGYLHTRYAE
                     RVRSSAGAVVSAVGRGKVVAFADNTQFRAFWFGANKLLANAIFFGHTIHPDASERSLP
                     KE"
     gene            9579..>11064
                     /locus_tag="PK198_10155"
     CDS             9579..>11064
                     /locus_tag="PK198_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRF39141.1"
                     /translation="MHEHIENLLALLRLEKEEELEFYRKKIQRLSLEERKAEGYCWYP
                     VKLAQQGYTIGDRAFVVVERPAENRKEHFFREGKTVNFFTRKEGARRAEFAGVVHFVD
                     KNKMKIILNSQDLPDWSDEGLTGVDLLFDERSYAEMEKALRQLMEVKKGRLAELRAVL
                     AGSKEPEFGQTPDIQHIPGLNASQTAAIRETLAARDVAVIHGPPGTGKTTTIVQAVKL
                     LCQTEHTVLVCAPSNAAVDLLTERLAAQGLFVVRLGNISRVDEGILSHTLEALAASHP
                     ESKNVKKVRIQAAECRRQARRFRRQFGSEERSERRQLLEEASQLAAWADALEDRIVEQ
                     ILDGAQVVTATLVGAAQKVLDKRHFRTLIIDEASQALEPASWIPILKASRVILTGDPC
                     QLPPTVKSEAAARQGLSVTMMERCIERMPWSPMLEVQYRMHAAIMGFSNRRFYKNRLK
                     AHESVSGHRLDIGEHFPLEFIDTAGCGFDEQVHPDSMSRFNPDEY"
     assembly_gap    11065..11084
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11085..>11319
                     /locus_tag="PK198_10160"
     CDS             <11085..>11319
                     /locus_tag="PK198_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRF39142.1"
                     /translation="EHLYQLIEAHHDRPLPTVAIISPYREQADHIRREAEQDAALAML
                     SLSVSTIDGFQGQERDVVYISLVRSNTKGEIGFL"
BASE COUNT         2401 a         3207 c         3008 g         2423 t
ORIGIN      
        1 tctggggcgc ggtttatggg acggccttta cgctcattgc gctttcatgg gcgcttccgc
       61 atttcctttc tgattttctt cagcgacttt tggaagggcc gacatcgccg ttttaggtcc
      121 aattttttat ggcgccccac gccaccggcg ccattccgtt tctatccctg gcgcgaccgg
      181 cgttgggggt tcatggcttc gctgaggccc tcgcccaata aattgaaaat ggtgacggtg
      241 ataaaaatgg ccagccccgg ccccaatgcc agccaccagg cccnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnncccag gttacttcct ccggcgacaa gccgatgccg
      361 agaaacgaca aaaacgcctc cagcaaaata gcgcccgcta tgccgaaagc caacgcaatc
      421 agcaccggcc caagggcatt gggcgccgca tgacgccaca aaatccgcca ctcgctcagg
      481 cccagcaccc gcgccgattc tatataaccc agactgcgga tgcgcaacaa ctccgcccgg
      541 atgaaccgcg caatgcccgt ccagcgtatc agcccgatga tgagcatggt ggccgccagg
      601 gagggtttgc gcagcaccgc cgctaccgac agcaacagca acagcgccgg tatggaattc
      661 atcacttcta tgaggcgcag caccagcatg tcggcaggca gataaacgcg gcgtttcagg
      721 aagggaattt tggacaatag ccaatacaaa gcattggcga tcaatagtat agttgcaaaa
      781 ataagcacag cctgccccca tccgcgaaaa gcgccgtcgg ggccgggcac ggtagcataa
      841 aacagccctg ccgctcctac gatcaaattc aacaccacgc ccgcgatgga aaccttgagg
      901 ctccggtcgc cgaaaaaacc ggccaccgcc cccagcaaaa tgccgatgag cagcgccacc
      961 cccatcgaaa ccaaacccac cagcatggcc gtacgcgcgc cccagatcat gcccgccgcc
     1021 acatcgcgcc cgatctgatc ggtgcccatc cagtgccgga agcgccacga ttcgacgcgc
     1081 tgcttgccga aaggattgac gtagttgaag ttctttttgt ctaaggcttg gggggaataa
     1141 ggtattgggg gaaacacgac gcgctcgtag ctgaggttct tccagtcgcc gccggccaat
     1201 gcgccgtccc agcgcgccca acccgctact actaagtaat ggcgcaatac gggaaagtaa
     1261 gtttgaccct cgatgcggca gtaaagcggc ttttcattgg caataaaatc ggcaaaaaca
     1321 gcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnaatcgca tgggaagagg cgttttttta gtgcatgggg cagccgcagt
     1501 cgccggaacg acacgcagaa attacaaaga gtaaacacaa acttaacagc cagtaaatga
     1561 ttcgagactt catgtcgttg tacttttgtt ccatttattt caccgacgca aatgtaaaca
     1621 atgagcaaca aacttcgcat tcttgtagcg cctttagact ggggcattgg ccatgctacc
     1681 cgctgtgtgc ccattattcg tgaacttttg cgacagggcg ccgaggtcta tatcgcctcc
     1741 aatggccgcc cgctcaaggt attacagcgg gagtttcccg aattgcccac cctcacgctg
     1801 ccccgctacg atgtgaaata tcctttccgc agcatcattc tgaacatgct gttgcaactg
     1861 ccccgcctgc tgtgggccat ctttgccgag catcgtatgg tggaccgctg ggtgcgcagg
     1921 tatggttttg acggcatcat caccgacaac cgtctgggct gctattcccg ccgggtgcct
     1981 actgttttca tcacccatca ggtacacctc aaagcgcccg atgaaaacat agcccgcttc
     2041 accaatcgca tcaatcattc cttcatgcgc cgctatggcc gctgctggat tcccgactgg
     2101 gacggctcgc aaagtctttc cggcacgctt tcgcgcccgt cggcacaccc gcacaccgtt
     2161 tacactggcc cactgacgag gctgcgacct gtgcagtcag caataaaata cgatgtgctg
     2221 gtgctgttgt cgggccctga acctcagcgc acttatgtag aaaaagccat cctgaaacaa
     2281 gccgccgggc tgccgtacaa tatgctgttg gtgcagggca aacccgaaac cgaccatacc
     2341 atacaaggcc ttgacaatgt ggaaatcgta ccctttttgg atgcgcccga actgagccgg
     2401 gccatggccg aatcgcgcat ggtggtgtgc cgttcggggt acagcacggt gatggatctg
     2461 gcggtgctgg gcaaaagagc gctgtacatt cccacgccgg gccagcctga gcaggagtat
     2521 ttggcggaat acctcggcga aaggggcgcg tcgttgtggc aggatcagga agagattcga
     2581 ttggccgagg gcatagccgg ggtgctggga ctgccggagc cggatatgtc gttggtgagc
     2641 cggcgtttgc tggaaacggc ggtgcgcgat tttttgccgg cggcttcgcc ttcggaagat
     2701 cagatgagtt gggcgacggc gtgaggcagg cgagcgcgcc aggnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nncgcggtgg ccgccataat agcggatggc ccgccacgcc acctgtggca
     2881 ccctgcgttg actgtgcccg ggtggcctcc tgcggcgtgg ggcgccccca ataaatcctg
     2941 gacttttttc tgaaaaatta tttttcaagg ttgtgttttc aaaaaaaacc taattttaac
     3001 ggcgaattag taaccatcat tctcttacga aaacacagtc aaaagcatga gtgcaggatt
     3061 aattactctg atcgtgggtt gggtatttgt tttggtgtgg gtgccttttg tggtactttc
     3121 ccaaagaaaa atgcacccca aagaattgtt caccctcttc tttgccgaga tgtgggagcg
     3181 cttttccttt tatggtatgc gcgccttcct cattttgtac atggcatccg aaattttcaa
     3241 acaatttggc cagaccgacg ccgacacgaa atcgtatggt gtgtatggcg ccttcaacgc
     3301 tttgctgtac gccgccccca ttgtgggcgg gatgctggcc gaccggatga tcggcttccg
     3361 caaggcagtt atgttgggtg gtattttcat ggcgctgggg cagtttacgc tggcggccaa
     3421 cgcctccatg ggcagccagt cgctcttgtt ttatggcggt ttgggcctgc tggcggtggg
     3481 caacggcttt tttaagccca atatttccag cttcctcggc actttctacg accgcaacga
     3541 caaccgcaaa gacggtgctt tcaccatctt ttatatgggc atcaacgtgg gcgcgtttct
     3601 ggccccgatc acttgcggtt atctggctcg cgaggtacac tactcgctgg gctttttggc
     3661 ggcaggcgta ggtatgttgc ttggcttggt tgtattttgg aacaacatcc ccaaagacaa
     3721 aggcaatgcg cctgatcccg tggcggcggc caaggcggta ttcatgggtt tgaatcgctt
     3781 ttgggttatt gtggctgcgt cgctggcgtt cattcccgtt tttgcactct tgatcggcgc
     3841 tgaaaaagtg accaactgga ttctcattat cgcaggtttg ggcgttatcg gctatcttat
     3901 tttctcagcg ctttcctctg aggacaagaa agagggccaa cgtttgctgg tgttcattgt
     3961 gctgttcttc ttccacatga tcttctggac cttgtttgaa caggcgggcg gatcgctcaa
     4021 catcctcacc gaccggtatg tgaataaaat gggcattgaa acctcgcagt tccagtctgt
     4081 caacccgctg tttatcatcc ttttagcccc tgtcttcacc tggatatgga cgcaattggg
     4141 caagcgaaag caggagccgc gcacgccgat gaaattcgtt tttgccctgc tgcaaatggc
     4201 cttaggttat ttcatcattg tgatcggcgc caaatccgta ctgggcgatg tggacaacgg
     4261 cggcatgatt ccgctctact tcctgctgtt catgtatttg ttccatacca caggcgaatt
     4321 gagcctctct ccggtggggc tttcggtagt gaccaaactc tccccggcca aaacagtagg
     4381 ctttgtaatg gggtcctggt tcctctccat cgctttcgcg cataagatcg gcggctatct
     4441 cggccaattg atcgctacgc cacaggaagg cgcttccaaa gcgagcgaac tgactgcctt
     4501 catcaatgtg tatatgaact ggggtgtgta cttggtattg ggttgcgccc ttctgttgtt
     4561 gttgcttact cctgtgctca aaaagtggat gcacggcgtg cattaagcat tgagcacaac
     4621 gccaaccaac gccggaggga gcaattttgt accgaggaaa aaaagtcggg tttcaaaatt
     4681 tctgcctccg gcgtttttgt ttttatcatt gagtcagtca tttaaaatca ccatatagct
     4741 tcaagccata agtcgtaagc cgcaagccgg gtgattcaaa ggtgtaagtt ttttcatccc
     4801 gggaaagcga taggtggttc aatgcacagc ttgtagcttg tagcttaagg ccggcagccg
     4861 atgattccaa cctgaaagaa actcgaaaaa catcctaatc atggaaaacc aacaacaaga
     4921 cttcaaaccg tacatctcgg ccaaggactt cataccggag tttacgctca aggccatttt
     4981 gctcggttcc ttcttcggcg tgctgtttgg cgcagccacc gtttatctcg gcctcaaagt
     5041 gggcctcacc gtgagcgcct ccatacccat tgccgtattg gccatttcca ttttcaaaaa
     5101 aataggcaag gccaccattt tggagagcaa catcgtgcag accatcggct cggccggcga
     5161 atcggttgct gccggcgtgg tgttcacgct gcccgcgctg ctgttcctca ccggcggacg
     5221 cgcgtatttc caatacttcc agatatttgt gctggccctc atcggcggca tattgggcgt
     5281 gttgttcatg gtgccgctgc gccgttcgct catcgtgaaa gagcacggca aactccccta
     5341 ccccgaaggc acggcctgcg ccgacgtgct ggtagccggt gaaaagggcg gcaaattggc
     5401 ccaaaaagtg tattacggtt tgggcgtggc gtttttgtac aaactcctca tgtccatcat
     5461 cggcttgtgg aaagacgtgc ccacgctgct gttcaagcgc ggcagttcgc tacccaacgg
     5521 ctcggtgagc gccgaaatca cgcccgaact gctcggcgta ggctacatca tcgggccgcg
     5581 catagccggc atcatggcag ccggcggcgt attgtcgtgg ttggtgctca ttccgctcat
     5641 caccattttc ggcgacggcc tggccggccc cctggcgcct gaaatgaccc aacgcattgc
     5701 cgatatgggg ccgggcgaca tctggaaccg ctacatccgc tatatcggcg ccggcgcggt
     5761 caccttcggc ggcttggtca cgctcatcaa aaccattccc accatcgtca gcgccttccg
     5821 cgattctttc aaagacctca aagaaagcag agcctccaaa ggcgtgcagc gcgagcgcac
     5881 cgaacgcgac atccccttgg tgtatgtgct ggtgggtagc gccgtgttgc tggtgctgat
     5941 gaccatactg cccaatgtgc ccaccaattt cctctcggcc atgctcatca tcgtgttcgg
     6001 ctttttcttc gtcacggtgt cttcgcgcat tgtgggcctc atcggttcgt cttccaatcc
     6061 catttccggt atgaccattg ccaccctcat ggccacctcg ctcattttcc tcggcgtggg
     6121 ctggagcggc gacgcgtacc aacccattgc tttggtggta ggctccatcg tgtgcatcgc
     6181 tgcggccaac gcgggcgcca catcccaaga cctgaaaacc ggctacatcg tgggcgctac
     6241 gcccgtcaaa cagcagttgg gcctcatcat cggggtgctg gtttcggtgg tggtcatcgg
     6301 tttcaccatg ttgttgctgg agcgcaccat cggcatcggc gaggccacag cggagcatcc
     6361 caacccgctg cccgccccgc aagccacgct catggccacc gtcatcaagg gcttgttgag
     6421 ccaaaacctg ccctggggat tggtcatcgc gggtatgggc attgccgccg tggtagagtt
     6481 gtgcggcgtc aattcgctgg catttgccgt gggcgcttac ctgccgcttt ccacctccac
     6541 gcctttgctg gccggcggtt ttgtaaaatg gatagccgac aagatcaaca aatccgacaa
     6601 agaagaatcc gacatcgggc cgggcgcgct gttcagttcg ggcctcatcg ccggcggcgc
     6661 catcatgggc atcgtcatcg ccatcctcat cggcagcacc tacggagtgg acgcagaggg
     6721 caagccgcgc tccttgctca gctacctcaa cattggtctg ggcgaaagcc tcggcagcgg
     6781 cggcgatctg ctgggcctca tcgcctttgt gttgctgggc gcagtgttgt tgcgctttgc
     6841 cacgcagaag gagaaggcat aggtatgccc atcgtggcca tttttaccag agacagaaaa
     6901 aagtcgtaag ccatttccaa aaaaatttgt tcctttccgc agctttgggg ttcattcttc
     6961 aaagctgcgt ttttttagca aaacatacca gctaccatga agcacacttt tacttttctc
     7021 ttgctttggg ccgcccttgc cgctttcgcg caaaaacagc cgctcactta cttcctgccc
     7081 gacatcccgt acgacaaaaa cctgcccacg cccgaagctt acctcggctt tcagatcggc
     7141 gagtggcaca tctcgcacga tcaactgctg ggctatatga aaacattggc cgccgcctcg
     7201 cccagagcca aattggtaga gcatggccgc acttttgaaa accgaccgct catccacctc
     7261 atcattactt ctgaaaaaaa tcacaagtcg ttggacgaga tcaaagccca gcatgtggcc
     7321 ctgagcaacc cgtccaaatc ggccggtgtg gacctcaata aagtacccgc cgtactctac
     7381 cagggctaca ccatacacgg caacgaaccc agcggcagca atgccgccat gctggcagcc
     7441 tactacttgc tggccggcaa aagcaaagac ctcgaccgcc tgctcgaaga cgtcatcatc
     7501 atttttgatc cctgtttcaa tcccgacggc atgaaccgtt tcgcctcctg ggtgaacacg
     7561 caccgcaata aaaacctcag ttccgaccct gccgaccgcg agttcaacga accctggccc
     7621 cgcgggcgca gcaatcacta ctggtttgac ctcaaccgcg actggctgcc ggcgcaattg
     7681 cccgaaagcc agggccgcgt gcgcatcttc cacgaatgga aacccaacgt actcaccgat
     7741 caccatgaga tgggttccaa caccaccttc ttcttcatgc ccggcgtacc ctcccgcgta
     7801 caccccctga cacccaagct caatcaggaa ctcaccgagc gcatcggcaa ctaccacgcc
     7861 gaagctttgg acggcatagg ctcgctgtac ttcaccaaag agggctacga cgatttttac
     7921 tacggcaaag gctccactta tcccgacgcc aacggttgtg tgggcatcct ttttgagcag
     7981 gccagttcgc gcggacatgt gcaacaaacc gacaacggcg acctcacctt tgccttcacc
     8041 atccgcaatc aggtggctac ctcgctttcg acccacaaag cggtgcgcga gatgaaaaaa
     8101 gacctgctca gctatcagcg cgatttttac acctccgccc tcgaagatgc ccgcaacgac
     8161 acccgcaaag cctacatttt cggcgatccg gccgaccgcg cccgcaccgc tgcttttatt
     8221 gagattttgc gcaagcacca gatcgaagta tatacgttga aaaaagatgc tactgccgca
     8281 ggtcatcagt ttacggcagg ccaagcctat gttgtgccgc tcgaccagcc gcaataccgc
     8341 ctcattcatg gcatttttga aaccagaacc gcttttgaag acagcatttt ttacgacata
     8401 tcgggctgga cttacccgct ggcctttaac ctgccgtatg ccgcgctgga aaaacgcgcc
     8461 tttgacgacg acatgatagg cgacaaaatt gccggtccgg gcatcgttgt ggaaacaccc
     8521 gcgcccgaat tcagcgagta cgcttacctc atggaatgga ccgaatacta cgctcccaaa
     8581 gcgctcaact acctgctgca acagggtctt cgtatgaaag tggcctctac gggcttctct
     8641 atggacaacc ggaattatcc gcccggaacc atcctgctgc cggtagctgg ccagagcaaa
     8701 acgccggagc agattcacgg actcatcacc attgcctcgc agatgggcgg cgtgcggttt
     8761 tatgatatgg acaccggtct gacgcctgtg ggcattgacc tcggcagcga cgatttcaag
     8821 ctcctcaaaa ccccagaagt gttgctcctc gtgggcgagg gcgtgcgcag caccgatgcc
     8881 ggcgaaatct ggcacctgct cgatcagcgc tacgatatgc gcgtgaccaa ggtggaaatg
     8941 acggcgctgg gccgcgttga tctgaacaaa tacacggcag tcatcatgcc cggcggcaat
     9001 tacggcagca tttccggcgg cgaccggctc aaagagtggg tgcaacaggg cggcgtcatc
     9061 gtggcctacc aggaggcagt gcaatggctg cgcagcaatg gcctggcgtt cgtggaattc
     9121 aggcaggaga aggcttccga cgctactccg gcacgccgtc catatacaaa aagggaggaa
     9181 gaccggggcg cgctgcaaac ttcgggggct atttttgaga acctgctcga caacggtcac
     9241 cccatcggtt acggctacac gcgagacaag atgccggtgt tccgcagcgg caatctctat
     9301 tttgaaccgg ccaaaaatcc ctacgccaca cccctactct actcctccaa tccgctgatg
     9361 agcggctacc tgcatacccg ctatgcagag cgcgtgcgca gttcggcagg cgcggtggtg
     9421 agtgcggtgg gccgcggcaa agtcgtcgct tttgcagata atacgcagtt ccgggctttc
     9481 tggtttgggg ccaataaact gttggccaat gccattttct ttggtcacac catccacccg
     9541 gatgcatcgg agaggagttt gccgaaggaa tagcgattat gcacgaacat atagaaaacc
     9601 tgctcgccct gctccgattg gaaaaggagg aagaattgga gttttacagg aaaaaaattc
     9661 agcggctgag cttggaggag cgcaaagcgg agggctattg ctggtacccc gtgaagctcg
     9721 cgcagcaggg ttataccatc ggcgaccgcg cttttgtggt ggtggagcgg ccggctgaaa
     9781 accgcaagga gcattttttt cgggagggca aaaccgtcaa ttttttcacc cggaaggagg
     9841 gcgcgaggcg ggcggagttt gccggcgtcg tgcattttgt ggacaaaaac aagatgaaaa
     9901 ttattctcaa cagtcaggat ttgcccgact ggagcgacga gggcctcacc ggggtggacc
     9961 tgctgtttga cgagcgcagc tacgcggaaa tggaaaaggc cctgcggcaa ctgatggagg
    10021 taaaaaaagg ccgcctggcc gaactgcgcg ccgtgttggc cgggagcaaa gaacccgaat
    10081 ttgggcagac gcctgatatt cagcacattc cgggactcaa tgcctcgcag actgccgcca
    10141 tccgcgaaac gctggcagcg cgcgatgtgg ccgtcattca cggtccgccg ggcaccggaa
    10201 aaactaccac cattgtgcag gccgtgaagc tgctgtgcca aaccgaacac acggtattag
    10261 tatgtgcgcc cagcaatgct gcggtggatt tgctgaccga acgattggcg gcgcagggct
    10321 tgtttgtggt gcgcctcggc aatatttcgc gcgtggacga gggcattctc tcgcacaccc
    10381 tcgaagcgct ggcggcctcg cacccggaga gcaaaaacgt gaaaaaagtg cggatacagg
    10441 ccgcagaatg taggcggcag gcccggcgat tcaggcggca gtttggctct gaggagcgca
    10501 gcgagcgccg ccaactgctg gaagaagcct cacaattggc cgcctgggcc gatgcgctgg
    10561 aagaccgcat tgtggagcaa attctggacg gcgcacaggt ggtgacggct actttggtgg
    10621 gcgctgcgca aaaggtattg gacaagcggc attttcgcac gctcatcata gatgaagcct
    10681 cgcaggcgct cgaacccgcc tcctggatac cgatcctcaa agcctcgcgg gtgatcctga
    10741 cgggcgatcc ctgccaattg ccgcccaccg taaaatcaga agcggcggcg cgacagggcc
    10801 tctccgtgac catgatggag cgctgcatag agcgcatgcc ctggtcgccg atgctggaag
    10861 tgcaataccg gatgcacgcc gccatcatgg gcttttccaa tcgtcgtttt tacaaaaacc
    10921 ggctcaaggc gcacgaatct gtgagcggcc accgcttgga catcggggag cattttccgc
    10981 tggagtttat agataccgcc ggctgcggat ttgacgagca ggtacacccc gattcgatga
    11041 gccgtttcaa tccggatgag tatcnnnnnn nnnnnnnnnn nnnncgaaca cctttatcaa
    11101 ctgattgagg cgcatcacga ccgtcccttg cctactgtgg ccatcatttc gccttaccgg
    11161 gagcaggccg atcacatccg ccgggaggca gagcaggatg ccgcgctggc catgttgtcg
    11221 ctgagtgtca gcacgataga tggttttcag gggcaggagc gcgatgtggt gtatatttca
    11281 ttggtgaggt caaacacaaa aggtgaaatc ggcttcctc
//
LOCUS       DAORLF010000104        11301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3082_length_11301_cov_2.129290, whole genome shotgun sequence.
ACCESSION   DAORLF010000104 DAORLF010000000
VERSION     DAORLF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_11301_cov_2.129290"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK198_10165"
     CDS             <1..848
                     /locus_tag="PK198_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRF39143.1"
                     /translation="LKIIATGSSAFDLSRQLGEPLTGRKWTFYMFPPSELEFSYDEDY
                     LTQQSRMQERLVLGSYPELLHLPDRQDKVAYLTEMVRDYLLKDILELEGLRNTHKITD
                     LLRLLAFQTGSEVSLSELGRQLSMDKNTVERYLNLLAEVFIIFNVRGYSRNLRKEITK
                     NSKWYFVDNGIRNTLISNFNPIELRNDQGALWENFILSERIKYQHYTRLMSNNFFWRT
                     YDQQEIDWIEERGGQLYGYEMKWSKPNAKAPTGWANAYPDAHFKVVHPGNFREFVIKD
                     DSAAI"
     gene            complement(851..1243)
                     /locus_tag="PK198_10170"
     CDS             complement(851..1243)
                     /locus_tag="PK198_10170"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF39144.1"
                     /translation="MAGLNDDTTLFLDTLNHPLRADIEVLRHLILSADDRLAENTKWN
                     APNYSVGGEDRVTMRIQPPRHLQVIFHRGAKKLDQPSTRIIEDNARLLAWKENDRAVA
                     TFKTLADMEKSRLDFIKIIQDWIEATCV"
     gene            complement(1255..>1766)
                     /locus_tag="PK198_10175"
     CDS             complement(1255..>1766)
                     /locus_tag="PK198_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRF39145.1"
                     /translation="LLAFRNGSLPVVVATDVLSRGIDVKGIDMVINFDVPGDAEDYVH
                     RIGRTARAEAMGEAITFINRKDRMRFGRIEKLIGRVIEKERIGGESGPSGDAQRQERG
                     ERRQRPPRRSNSSQQGNAPRQQRPPQQGDAGAGQASSAEQQKKKRRNKRKNRSGGPRP
                     DNAPPRQDS"
     assembly_gap    1767..1776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1777..2658)
                     /locus_tag="PK198_10180"
     CDS             complement(<1777..2658)
                     /locus_tag="PK198_10180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF39146.1"
                     /translation="MKFSEFGLTEALMEGLEAMRFDNATPIQEQAIPAIMEGRDVLAC
                     AQTGTGKTAAFLLPVLHRITETPPTGIDTLIIVPTRELAMQIDQQLEGFSYFTPVSAV
                     AIYGGRDGHSMELERNALKKGAPIVIATPGRFIAHLDLGYVNLSTVRHLILDEADRML
                     DMGFAPDINKITSQVPRSRQTLMFSATMPPQIRKFVKDLMRNPLEITIAVSRPAEKIR
                     QLAFEVEDEAKYPLVERLLAERENLERVIIFAGSKIEVREVAQRLSKRGMKVAAIHSD
                     LEQAERELGPQHPTHGFI"
     gene            2789..3217
                     /locus_tag="PK198_10185"
     CDS             2789..3217
                     /locus_tag="PK198_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39147.1"
                     /translation="MNTVTKRFLDCFEQLKSTNKVRSGRQFAVSLGYLPQSFSEIVNG
                     RREVTVELLRKATEQYRLNPAYLLSGLEPMILTDPAPATHNGAPAHNGFHGAPAHNGT
                     SVPDEIRTIKEMMMQQAEAIGQIQSFMEQWAPHPSDNNQP"
     assembly_gap    3223..3373
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3458..3835
                     /locus_tag="PK198_10190"
     CDS             3458..3835
                     /locus_tag="PK198_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF39148.1"
                     /translation="MQKQTLALLVSGLLLLSAFAGDTARPAEWTGVKDYDFGLLTRGR
                     PAQHRFVLKNKSSRPLLIDNVRTPCGCTAVDWPEMPIPPGDTASVLVEYNAAQVGYFY
                     KTVKVFFHGVKGPDVLSVQGEVE"
     gene            complement(3851..5254)
                     /gene="lpdA"
                     /locus_tag="PK198_10195"
     CDS             complement(3851..5254)
                     /gene="lpdA"
                     /locus_tag="PK198_10195"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRF39149.1"
                     /translation="MKFDVTVIGSGPGGYVAAIRAAQLGLKTAIIERYNTLGGTCLNV
                     GCIPSKALLDSSEHYHNAHEKFSQHGILLSNLQVDMPQMIRRKNEVVDQTVKGLDFLM
                     KKNKIEVFRGHGAFLGPNRIQITAADGAVTEIETDKTIIATGSKPITPPAFNYDKKRV
                     ITSTEALNIEQVPSRMVIIGGGVIGLELGSVFGRLGAKIDVVEFLDRIIPTMDGDCSK
                     ELMRSLKKVNMNFHLRHAVTSVKATDTGVEVEVQKRDSEEKFTIEADYCLVAIGRRPY
                     TDGLGLDKAGVQLDDKGRVLVNDHLETNVPGIYAIGDVIRGAMLAHKAEEEGVLVAET
                     IAGQKPHIDYNLIPGVVYTWPEVAAVGRTEEQLKEEGIPYKSGKFPFKALGRARASTD
                     VDGMVKVLSHKDTDEILGVHIVGPRAADMIAEAVALMEFRASAEDAARMSHAHPTYTE
                     AFKEAALAATANRAIHV"
     gene            complement(5264..6787)
                     /gene="ricT"
                     /locus_tag="PK198_10200"
     CDS             complement(5264..6787)
                     /gene="ricT"
                     /locus_tag="PK198_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRF39150.1"
                     /translation="MGCAGCSISKNTGADAKGCGGGCSSNGCSSGGCNRLNTFDWLTY
                     LELDDAEPFQYAEVSFKNGARKSFFRNPETARVSTGDWVLVESLNGYDVGRISLSGDL
                     VRLQMKRKRCNIDHVQQSIIRRANERDLERMQEARSRERDTIVLARAIARSLKINMKI
                     GDVEYQGDGRKATFFYTADGRVDFRELIRQYAREFSVKIEMRQIGARQESARIGGIGA
                     CGRELCCSTWLSDFKSVNTAAARYQNLAINQSKLSGQCGRLKCCLNYELDTYMDALKG
                     FPDDAEKLQTKEGVAVLVKTDIFRQLMFYAYEHEGGRGKIYALDAKQVHAIVEMNKNR
                     ERPTSLGDVQVVQASTRGEDEPEVDYSDVTGAIELPDEERRRRKKKKKKPQGRGGEGG
                     PQQGNRPGNPNAPREPRDNNRNQQNRPRQNGGDAEPGKNDGNNSGQRRNENRPPRRDD
                     NRPPKQSDGNNEAGKNEGNNSRPPRNENRPPRRKDRPGNNDRRPPRDDNNNNDQPKQ"
     gene            complement(6848..7981)
                     /locus_tag="PK198_10205"
     CDS             complement(6848..7981)
                     /locus_tag="PK198_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39151.1"
                     /translation="MLFSQVIGQKTVQAQLLRMVQSDRLPHALLLLGPPGSGKRTLAL
                     ALAQYVLCDAPGPTDACGACRSCRSVEGMKHPDLHFSYPTAGANAISEHFLDAWRTRM
                     AESPYFDVFDWIKTIGKESNQGNITKDECVNIVRKVSLKAFMGRRKILLMWLPEFLGK
                     EGNRLLKLIEEPPDDTLFVLLAENPERILPTILSRCQIVRTGPIDDADVSEGLQRIKG
                     VSPEQADTTALLADGNFYLALQLAGNQENNYADLFVEWLRACHQNSGIGWVKIAERIA
                     EIGREPQKYLITYGLHFLREALIHSLTDQLPPRLGGQQLVTAQKLMAAGLDAERIGAM
                     SALLTDCHYHVERNANAKVLFLNTSIRLHRIMRQPAEVWAATR"
     gene            8368..10179
                     /locus_tag="PK198_10210"
     CDS             8368..10179
                     /locus_tag="PK198_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39152.1"
                     /translation="MPGFPVSTFTSQQLSRWNLLQNENPNDTLYVMNPIDFTRMDSLS
                     RFKIALPGGCPTVFCQATKRVSYPNGDYVVLANVMENDDPYGACSTGDLSVVRHNGTA
                     VGMIRVDDRAFEIRDLKDGKWCLRAVKNSALPEYCISSGGGGGTFHEEHDNEQVETRN
                     TFCVIQVLVLFDAPSNVMGDIRARAIEDINKTNEALRNSGIFNENIRFELLDALEFNL
                     PNPQQFNVLEDLVRLRNENQALRGNLGADIVVYYSNVDYLAVLGQDVFDIFGAALGLE
                     LPGRQVIPERDAFVVVEATDDAKRRLTFQHELAHLMQCRHQEQNDPNGEFQHGHRFRY
                     RGGFLGWGRPAGHTIMHTIGIDENGRDEVRFLHYSNPAIEYRGAATGIAGIRDNARQI
                     RNVGCDVANWRTGAELSVFIEGPVSGCPFQSANLKAYITGNAPTPYIYTWEISTTSFM
                     GPFSPIGVSNPNISIPLGDVGETVYVRLTITAGNGMTITEFHEIRPEYAGDCYEYFFR
                     ASAGQTAQNTAVSMHVWPNPARERINTALKVESPQEYMYADLLSSDGAILQSQQWKDV
                     QVGTIYTWFQSTTLPPGIYRLRIVTPHGVVCNPIVKH"
     gene            10451..10741
                     /locus_tag="PK198_10215"
     CDS             10451..10741
                     /locus_tag="PK198_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39153.1"
                     /translation="MKIGTYSIREYPEFLIDRGNYASYGTWLSDGDLVGEIYHSSSDT
                     AFHFFALDKYDPVKQEVEGRFQVELIKDPSRDFFDTPDTLRFTNGRFKLRVR"
     gene            complement(10802..>11301)
                     /locus_tag="PK198_10220"
     CDS             complement(10802..>11301)
                     /locus_tag="PK198_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39154.1"
                     /translation="AALPVIRTYPNPAVDYLTSNIGGFNATNNGFMFIALKPKSERDK
                     ADVVIARLRRATAEVPGMTAVLGGIWLFCCLILLLPLLAIGQAVTSGYLLTCGCWASA
                     ALFFLIKTLSDRYLLGEAASFFKRPDLLHRFLYSEVLHLLYMTAIGLGGLLKKRYEWK
                     GRQVK"
BASE COUNT         2450 a         2910 c         3051 g         2729 t
ORIGIN      
        1 gtttgaaaat cattgctacc gggtcttccg ctttcgatct cagccggcaa ttgggagaac
       61 ctttgaccgg acgaaaatgg acgttttata tgttcccgcc gtcagagctg gagttttctt
      121 atgacgaaga ctacctcact caacagagtc gtatgcagga gcgcttagta ctgggttctt
      181 atccggagtt attgcattta cctgacagac aagacaaagt ggcctatctc accgaaatgg
      241 ttcgagatta cctgctcaag gacattctgg aactggaagg attgcgcaat acacacaaaa
      301 taacagactt gctccgcctt ttagcattcc agacaggcag cgaagtttct ctcagcgagc
      361 ttggcaggca attgtccatg gacaaaaaca ccgttgaacg ctacctgaat ctgctggcag
      421 aggtatttat catttttaat gtacgagggt actcccgcaa cttgcgaaag gaaattacca
      481 aaaattccaa gtggtatttt gttgacaacg gcatccgcaa cacactcatt tccaatttca
      541 atcccatcga attgagaaac gatcaaggcg ccttgtggga gaatttcatt ctgagcgaac
      601 gcatcaaata ccagcattat acccgtctca tgtccaataa tttcttttgg cgcacttacg
      661 accagcagga gatagactgg atagaagagc gaggcgggca gttgtatggc tatgaaatga
      721 aatggagcaa accgaatgct aaggcgccga cagggtgggc caatgcctat cccgatgccc
      781 acttcaaagt cgttcaccct ggcaatttca gagaatttgt gataaaagac gacagtgccg
      841 ccatttgaaa tcacacgcag gtagcctcta tccagtcttg tatgattttg ataaaatcca
      901 gccggctttt ctccatgtcg gccagggttt taaaggtcgc aacggcccgg tcattttctt
      961 tccaggccag gagacgggcg ttgtcttcga tgattcttgt ggagggttgg tcaagctttt
     1021 tggcgccgcg atgaaaaatc acttgtaaat gcctcggtgg ctgaatccgc atggtgactc
     1081 ggtcttcgcc tccgacgctg taattgggcg cattccattt ggtgttctcc gccagcctgt
     1141 cgtctgcgct caaaatcaga tgcctcagca cctcgatatc cgcacgaagc gggtggttca
     1201 gagtgtccaa gaacagcgtt gtgtcatcgt tgagaccagc catagtttag aattttatga
     1261 atcctgcctc ggcggcgcat tgtccggtct tggcccgccg ctgcggtttt tccgcttgtt
     1321 tctgcgcttc ttcttttgct gctcggcaga tgatgcctga ccagctccgg catcgccctg
     1381 ttgggggggg cgttgctgcc ggggggcatt gccctgctgc gatgaattcg acctgcgggg
     1441 cggcctttga cggcgctcgc cgcgctcctg acgctgcgca tcgcctgatg ggccggattc
     1501 gccaccgatt ctttcctttt caatgacgcg gcctatcagt ttttcaatgc ggccaaaacg
     1561 catgcggtct ttgcgattga tgaacgtgat ggcttccccc atggcctcgg cgcgggcggt
     1621 gcgcccaatg cggtgtacat agtcttcggc atcgccgggc acatcaaaat tgatgaccat
     1681 atctatgccc ttcacatcta tacccctgga gagtacgtcg gtagctacta caacaggcag
     1741 cgaaccgttg cgaaaagcga ggagccnnnn nnnnnngatg aagccgtgcg tcgggtgctg
     1801 cggaccaagt tcgcgctcgg cctgttcgag gtctgagtgg atggccgcta ctttcatgcc
     1861 ccgtttggag aggcgctgtg cgacttcgcg tacttcgatt ttggaacctg caaaaatgat
     1921 gacgcgctcc aagttttcgc gctctgcgag caggcgttcc accaatgggt atttggcctc
     1981 gtcttccacc tcgaaggcca actggcgtat tttttcggca ggccgggaaa ctgcaatggt
     2041 gatttcgagc ggattgcgca tcaggtcttt gacaaacttg cgaatctgcg gcggcatggt
     2101 ggccgaaaac atgagcgttt ggcgggaacg cggcacctgg cttgtgatct tgttgatgtc
     2161 gggcgcaaaa cccatgtcca acatgcggtc ggcttcgtcc aaaatgaggt gccgcaccgt
     2221 agagaggttg acgtagccca gatcgaggtg ggcaatgaag cggccgggcg tggcaatcac
     2281 gatgggcgct ccttttttga gggcattgcg ctccaattcc atcgaatggc cgtcgcgacc
     2341 gccgtaaatg gctaccgcac tgacgggcgt gaagtaggaa aacccttcga gttgctgatc
     2401 tatctgcatg gcgagttcgc gggtgggcac aatgatgagc gtatcaatgc ctgtgggcgg
     2461 tgtctcggtg atgcggtgta aaacgggaag caaaaaagcg gcggttttgc cggtgccggt
     2521 ctgcgcgcag gccaacacat cgcgcccttc catgatggcc ggaatggctt gttcctgtat
     2581 gggggtcgca ttatcgaagc gcatggcttc aagcccttcc atgagggctt cggtaagtcc
     2641 gaattctgaa aatttcaaag tgtcaatatt aagttaggac gcaaagatac aggattttag
     2701 gcaaaaattt ttgcccgcag gcattgccgg gtgcaataaa gtactatttt tgtttgacgc
     2761 agcgcccgtt tagcctcgtc gcctatatat gaacaccgta accaagcgat ttttagactg
     2821 ctttgagcaa ctcaaaagca ccaacaaggt gcgttcgggg aggcagtttg ccgtgtcgct
     2881 gggttacctc ccgcaaagtt tttccgaaat cgtcaatggc cgtcgggagg tcacagtaga
     2941 gttgttgcgc aaagccacag agcagtaccg cctcaacccc gcttacctgc tgtcggggct
     3001 ggagccgatg attctgactg atcctgcccc ggccacgcac aacggcgccc ccgctcacaa
     3061 tggcttccac ggagcgcctg ctcacaacgg cacttctgtt cccgacgaaa tccgaaccat
     3121 caaggaaatg atgatgcaac aggcggaggc catcggacag attcagtctt ttatggaaca
     3181 atgggcgccc catccatcag acaacaacca accataacca atnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnagaagcg gatgagcagt tcgatatttt tatgtggggc actcctgtaa
     3421 aaatcgttgc ctttcacacg gtaaatcctt gattttcatg caaaaacaaa cccttgccct
     3481 gcttgtgtcc ggcctgctcc tgctgtctgc ctttgccggc gatacggccc ggccggcaga
     3541 atggacgggc gttaaagact acgatttcgg gttgctcacc cgcggccgcc cggcacagca
     3601 tcggttcgtt ttaaaaaaca aaagctctcg cccattgctc atagacaacg tacgcacgcc
     3661 ctgcggctgc accgccgttg actggcccga aatgcccata ccaccgggag atacggcctc
     3721 cgttttggtg gagtacaatg cggcgcaagt cggctatttt tacaaaaccg tcaaagtgtt
     3781 tttccacggc gtcaaaggcc cggacgtact cagcgtgcag ggcgaggtgg agtgagaaag
     3841 cacttccccc tcagacgtgt atagcccggt tggccgtagc cgccaatgca gcttccttaa
     3901 acgcctctgt ataagtagga tgcgcgtgac tcatgcgggc ggcgtcttcg gcggaagccc
     3961 gaaactccat cagcgccact gcttctgcaa tcatgtcggc agcgcggggg ccaacaatgt
     4021 gtacgcccaa tatctcgtcg gtatctttgt ggctgagtac cttcaccatg ccgtccacgt
     4081 cggtgctggc gcgggcgcgg cccaatgctt tgaacggaaa cttgcccgat ttgtaaggaa
     4141 tgccttcttc tttgagttgc tcctcggtgc gccccacggc ggccacctcc ggccaggtat
     4201 aaaccactcc cggaatgaga ttgtagtcaa tatgcggctt ctgaccggca atcgtctcag
     4261 caaccagcac gccttcttct tcggctttgt gcgccagcat ggccccgcga atcacatcgc
     4321 cgatggcata aataccgggt acattggttt ctaaatgatc gttgaccaag acgcggcctt
     4381 tgtcgtccaa ctgcacgccg gctttgtcca agcccagccc gtctgtataa gggcggcgac
     4441 caatggccac gaggcagtaa tcggcctcaa tcgtaaattt ttcctccgaa tcgcgctttt
     4501 gcacctccac ttccacgccg gtatctgtgg ctttcaccga agtgacggca tggcgcaggt
     4561 ggaaattcat gttgactttt ttgagcgaac gcatcagttc tttgctgcaa tcgccgtcca
     4621 tggtaggaat gatgcggtcg aggaactcca ccacgtcaat tttggcgcct aacctgccga
     4681 aaacagagcc gagttcgagg ccgatgacgc cgccgccgat gatgaccatg cgcgagggca
     4741 cttgctcaat gttgagcgct tcggtggagg tgatgacccg ctttttgtca taattgaaag
     4801 caggcggcgt gatgggcttt gaccccgtgg caatgatcgt tttgtcggtt tcgatttccg
     4861 tgacggcgcc gtcggcggca gtgatttgga tgcggttcgg gccgaggaag gcgccatgcc
     4921 cgcgaaaaac ttcgattttg tttttcttca tcaaaaaatc gaggcccttc acggtttggt
     4981 ccaccacctc gtttttgcgg cgaatcatct gcggcatgtc cacttgcaga ttgctgagca
     5041 gaatgccgtg ctgcgagaat ttttcgtgcg cgttgtggta gtgctcggag gaatcgagca
     5101 gggctttgga tgggatgcaa cccacgttga ggcaggtgcc gcccaatgta ttgtagcgct
     5161 ctatgatggc tgttttgagg ccgagctgtg cggcgcgaat ggcggccaca tacccgcccg
     5221 ggcctgatcc gattacggtt acgtcaaatt tcatgatgag aaactactgc tttggttggt
     5281 cattgttgtt gttatcatcg cgaggcgggc gccgatcgtt gttgccgggc cggtctttgc
     5341 ggcgcggcgg gcggttttcg ttgcgaggcg ggcgggaatt attgccctcg ttcttaccgg
     5401 cctcgttgtt cccgtcactt tgtttcggcg ggcggttgtc gtctcggcgc ggcgggcgat
     5461 tttcattgcg gcgctggccg gagttgttgc cgtcgttttt gcccggctcg gcatcgccgc
     5521 cgttttgtct cgggcggttt tgctgattcc ggttgttgtc tctcggttcg cgcggagcat
     5581 tcggattgcc ggggcggttg ccttgctgcg ggccgccttc gccgcctctg ccctgcggtt
     5641 ttttcttttt ctttttgcgg cggcggcgtt cttcgtcggg cagttcgatg gccccggtca
     5701 cgtcgctgta atccacttcc ggttcgtctt caccacgggt actggcctgc accacctgca
     5761 catcgccgag actggtgggg cgctcgcgat ttttgttcat ctctacgatg gcgtgtacct
     5821 gcttggcatc caatgcataa atcttgccgc gtccgccctc atgttcatac gcataaaaca
     5881 tgagctggcg gaagatgtcg gttttcacca aaacggccac cccttccttg gtttgcagtt
     5941 tttcggcgtc gtcggggaag cccttgagcg cgtccatgta ggtgtccaac tcgtagttga
     6001 ggcagcactt gaggcgaccg cattggccgg agagtttgga ctgattgatg gctaagtttt
     6061 gatagcgggc cgccgccgtg ttcaccgatt tgaaatcgct gagccaggtg gagcagcaca
     6121 attcgcggcc acaagcgccg atgccgccga tgcgggccga ttcctgccgg gcgccgattt
     6181 gccgcatttc tattttgacg gaaaactcgc gggcatactg gcgaatcagt tccctaaagt
     6241 ccacgcgacc atcagcagtg tagaaaaaag tggctttgcg accgtcgccc tgatattcca
     6301 cgtcgcctat tttcatattg attttgagcg aacgggcaat ggctctggcc aatacgatgg
     6361 tatcgcgttc gcgggaacgc gcctcctgca tacgctccag atcgcgctcg ttggcacgcc
     6421 ggatgatgct ttgttgcaca tggtctatat tgcagcgttt gcgcttcatt tgcaggcgca
     6481 ccaaatcgcc cgaaagggaa atgcgcccca catcgtagcc gttgagggat tccacgagca
     6541 cccagtcgcc ggtgctgacc cgcgccgttt cggggttgcg gaaaaaactc ttgcgggcgc
     6601 cgtttttgaa actgacctcc gcatactgaa agggttcggc gtcgtcgagt tcgaggtagg
     6661 tgagccagtc gaaagtattg aggcgattgc atccgccgct cgaacagccg ttggaggagc
     6721 atccgccgcc gcatcctttg gcatcggccc ccgtattttt agaaattgaa catcctgcac
     6781 atcccattat ttttcaacaa tttatgatgg atttatgata aaaaggcccg gcttttcggg
     6841 ccgtgattca gcgtgttgcc gcccatactt ccgccggctg ccgcatgatg cggtgcaagc
     6901 ggatggacgt attgagaaac aaaactttgg cgtttgcgtt gcgctccaca tggtagtggc
     6961 agtcggtgag cagcgccgac atagccccga tgcgctcggc atctaagccg gcggccatga
     7021 gcttttgagc cgttacgagt tgttgacctc cgagccgcgg cggcaattgg tcggtgaggc
     7081 tgtgaatgag tgcctcgcgc aaaaaatgca gcccgtaagt gatgaggtat ttttgcggct
     7141 cgcggccgat ttcggcgatg cgctctgcta tttttaccca accgatgccg gagttctggt
     7201 ggcaggcacg caaccattcc acaaacaaat cggcgtagtt attctcctga ttaccggcca
     7261 attgtaaagc cagataaaag ttgccgtcgg ccagcagggc ggtggtgtct gcctgttcgg
     7321 gagagacgcc tttgatgcgt tgaagccctt cggagacatc cgcatcgtca atgggacctg
     7381 tccgcacgat ctggcatcgc gacaaaatgg tgggcaaaat gcgctccgga ttttcggcca
     7441 gcagcacgaa cagcgtgtcg tcgggcggct cttcgatgag tttgagcagg cgattgcctt
     7501 ctttgcccaa aaattcgggc agccacatca gcaaaatttt ccggcggccc atgaaggctt
     7561 tgaggctcac cttgcgcacg atgttcacac attcgtcttt ggtgatgttg ccctgattgc
     7621 tctctttgcc gatggttttg atccagtcga acacatcaaa gtaaggcgat tcggccatgc
     7681 gcgtgcgcca ggcatccaga aaatgctcgc tgatggcgtt ggccccggca gtggggtagg
     7741 aaaaatgcag atcggggtgc ttcatccctt ctaccgaacg gcaggaacgg cacgctccgc
     7801 aggcgtcggt ggggccggga gcatcgcaaa gcacatattg cgccagggcc aaagccagtg
     7861 tgcgcttgcc cgatcccgga ggccccagca gcaagagggc atgaggcagc ctgtcggact
     7921 gcaccatgcg gagcaactgc gcctgcactg ttttctggcc tatgacctgc gagaagagca
     7981 ttttacttca aggtgttgat gttcgggcgg taaagataag tgttttggga agatttccgc
     8041 agtcttccga acgctgtaag ttttgatctg cgaaggagca tgtcccgcaa tggcatagcg
     8101 acgattttgg cgggaggtaa agccttcggg ataagcatat tgcttgcctt gtattttgta
     8161 gggtaaaaag ttgaattaaa acataggcac aaaatagtgt aatgaatttc aagcgatttc
     8221 ctacctttgt aactgaacta aaaaatctta ccgttatgat caacttatcc ctcacggctc
     8281 acggctcacg gctcacggct cacggccatt ctgatccttt ttcttgccgg cttcggccca
     8341 acgtacgctc aatcgctgat tgagattttg ccgggttttc ctgtttcgac gttcacatcg
     8401 caacaactca gccgatggaa cctgttgcag aacgaaaatc ccaacgacac gctctatgtg
     8461 atgaacccga tagatttcac ccgtatggat tcgttaagcc gattcaaaat agctctgccc
     8521 ggaggctgcc cgacagtatt ctgtcaggcc accaagcgtg tgtcgtaccc caacggcgac
     8581 tatgtagtgt tggccaatgt aatggaaaac gacgacccct acggcgcatg cagcactggc
     8641 gacctctcgg tggtgcggca taacggcacg gccgtaggca tgattcgggt ggatgatcgc
     8701 gcgtttgaaa ttcgggattt gaaagacggg aagtggtgtt tgcgggcagt aaaaaacagt
     8761 gctttgcctg aatactgcat ttcatccggc ggcggcggcg gaacgttcca cgaagagcat
     8821 gataacgaac aagtggaaac aagaaatacc ttttgcgtta ttcaggtgct tgttcttttc
     8881 gatgccccat ccaatgtgat gggcgatata cgagcgcggg caattgaaga cataaacaaa
     8941 accaacgagg ctctgcgcaa cagcggcatt tttaatgaaa atattcgctt cgagttattg
     9001 gatgcgcttg aattcaattt gcctaaccct caacaattta acgtattgga ggatttagtt
     9061 cgtcttagaa atgagaatca ggctttacgc gggaatttgg gggccgatat cgttgtctat
     9121 tactccaatg tggattactt agccgttttg ggtcaagatg tttttgatat ttttggcgca
     9181 gcgcttggtc tcgaactgcc cggccgccaa gtgatccccg aacgtgatgc ttttgtggtg
     9241 gtagaagcta cagatgacgc aaaaagacga ctgaccttcc aacatgaatt ggcacacttg
     9301 atgcaatgca gacatcaaga gcaaaacgac cccaatggcg agttccaaca tggacaccgt
     9361 ttccggtatc gaggtggatt cttaggatgg ggacgccctg caggccacac aatcatgcac
     9421 accattggta ttgatgaaaa cgggcgtgat gaagtgaggt ttcttcacta ttccaatccc
     9481 gctatcgaat acagaggagc agccacaggc atagccggca tccgtgacaa tgcccgccag
     9541 ataaggaacg tcggttgtga cgtagccaat tggagaacgg gggccgagct gtcggttttt
     9601 attgaaggcc ctgtttcagg ctgtccgttt caaagcgcta atctgaaagc atacattact
     9661 ggcaatgctc cgacgcccta tatttataca tgggaaatat ccactacatc attcatgggg
     9721 ccattcagcc ccatcggggt aagcaatccc aatatttcca ttccactggg agatgtgggt
     9781 gaaacagtgt atgtccggct caccattact gccggcaatg gcatgactat aacagaattt
     9841 cacgaaataa ggccggaata cgcgggagat tgctacgaat attttttcag agcatcggcg
     9901 ggccaaactg cacagaatac tgccgtctcc atgcacgtgt ggcccaaccc tgcccgagag
     9961 cgcatcaata ccgcgttgaa agtagagtcg ccgcaagagt atatgtatgc cgacctgctt
    10021 tcttctgacg gcgccatttt gcaaagccaa caatggaaag acgtacaggt gggtaccatt
    10081 tatacttggt ttcaaagtac gacactgccg ccgggaatat accgcctgcg cattgtgacg
    10141 cctcatggag tagtctgtaa ccccattgtc aaacactaaa aataaaacca ccatgagtag
    10201 aatattcatt gtatccctgt tctttgctgt tgcgatgctt ttttcttgta aaaaagatga
    10261 cgacgctagg ttgatacctg accttgatga atttaatagc catgtttctg cactgctcaa
    10321 tggagagccg tggtatgctt atatgaattg gaaacaggac ttgtacgttg acatagacac
    10381 taacgggttc tacttcgttg tgacaaagaa tggaattcct cgagaatcat taagttttga
    10441 ctggttgcct ttgaaaatag gtacttactc tatacgggaa tatccggagt ttttaataga
    10501 cagaggcaac tatgccagtt acggtacctg gctctcagac ggcgatctag taggcgagat
    10561 ataccattct tcctccgaca cggcctttca ctttttcgca ttagacaagt acgatcctgt
    10621 gaaacaggaa gtagagggcc gctttcaggt agaactgatc aaagacccat cccgcgattt
    10681 cttcgatacg ccggacacgc tgaggtttac caacgggcgc ttcaaactgc gtgtgcggta
    10741 ggtacgaggt gctgttgcat atatcaaaaa cggggaggca atgtcgtatt gcttccccgt
    10801 tctacttcac ctgccgcccc ttccattcat atcgcttttt caacagtcca cccagcccaa
    10861 tggctgtcat gtagaggagg tgaagcactt cggaataaag gaatctgtgc aggagatcgg
    10921 gccgtttgaa aaaactggcg gcttcgccca ggaggtagcg gtcggaaagg gttttgatga
    10981 gaaaaaacag ggctgctgaa gcccaacacc cgcaagtcag gaggtaacct gaagttaccg
    11041 cctgaccgat cgccagtaaa ggcaacagta aaatgagaca acaaaacagc cagatacctc
    11101 ccaacaccgc cgtcatgccg ggcacttcag cggtggcgcg gcgcaggcgc gcaatcacca
    11161 catcggcctt gtcgcgctct gatttgggct tcagcgcgat gaacatgaag ccattgttgg
    11221 tggcgttgaa gccgccgata ttcgaggtca ggtagtcgac cgccgggttt ggatacgtac
    11281 ggatgaccgg cagggctgca c
//
LOCUS       DAORLF010000105        11296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3085_length_11296_cov_2.130860, whole genome shotgun sequence.
ACCESSION   DAORLF010000105 DAORLF010000000
VERSION     DAORLF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_11296_cov_2.130860"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>374)
                     /locus_tag="PK198_10225"
     CDS             complement(165..>374)
                     /locus_tag="PK198_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRF39155.1"
                     /translation="GGSGDVLTGIITGLRAQGYAALDAAVLGVYLHGLAGDLAAKQKG
                     QEALIAGDLIEMMGAAFLHIQNTTT"
     assembly_gap    375..394
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<395..1695)
                     /locus_tag="PK198_10230"
     CDS             complement(<395..1695)
                     /locus_tag="PK198_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRF39156.1"
                     /translation="MKILSAEQTRALDAYTIANEPIASIDLMERASLTFVKWLVQPFP
                     DSDRKVLILVGPGNNGGDGLAVARLLHRRFYDVAVWRCHIGAAVSPDFQINWDRLPTE
                     GGAIAVHHLHAGDPLPDWPQGALLIDALFGSGLSRPVTGYWADLIRHCNEQSLTRVAI
                     DVPSGLFTDGPSEGAVLEAHYTFSFETPKLAFFLPDNQQRVGEWRFGSIGLDAGFLSE
                     ISTLFHFVEAASARNMLKTRRRFDHKGVFGHALICAGSYGKAGAAVLATRACLRSGAG
                     LVTVHTPRCAYPILQTAAPEAMTLADAHELHISEMPDLSPFSAIGAGCGWGQEELTAN
                     ALHQLLECAVQPLVLDADALNLLALHPEWMKLLPENTILTPHPKEFERMFGPSNNGFE
                     RLEILRKKAAELHVVIVLKGAFSCIASPDGHCYFNATGNPG"
     gene            complement(1711..>2619)
                     /locus_tag="PK198_10235"
     CDS             complement(1711..>2619)
                     /locus_tag="PK198_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF39157.1"
                     /translation="VADVRDCLGNPAGAAMSTRIGLAEAMTPGDLVINELLFNPRTGG
                     QDFIELYNTSAKILNLRGLQIQNTQRTTNFSTTFQSDFLLFPDEYAAISSDIANIQAY
                     YNVPNISALVQNALPGFDDASGNATLLFNGVLIDAFDYSEAWHSPLLDTKDGVSLERI
                     NSAGPSNSAGNWHSAAVAVGFATPGYRNSQFRISPSLADEMISLPNKTFSPDGDGYED
                     VLQIFYTADKPGFLLNLLIFDAAGRTVRTLLRSELLGSEGVFKWDGANDEGTKARTGI
                     YILWIELFHPDGQVERVKKTCVLAGY"
     assembly_gap    2620..2629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2630..>3234)
                     /locus_tag="PK198_10240"
     CDS             complement(<2630..>3234)
                     /locus_tag="PK198_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39158.1"
                     /translation="QLQDIRYSNGSSPQPLPNFLLLPGQYVIVCPQNNIAAFAPFGNV
                     AGMTSFPALTNSGDEIILSNAQGEELFYLEYDLSWYQDPAKTDGGWSIERIGNNLADN
                     CPANWRASVHPRGGTPGQLNSVQGTPNDNIGPRLLRAALSTPSSVIVTFDEPLSESSL
                     NAGWFSFSDGVGVISATLLTPATQVLLALSAPLMAGRSYTLT"
     assembly_gap    3235..3309
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3310..4319)
                     /locus_tag="PK198_10245"
     CDS             complement(<3310..4319)
                     /locus_tag="PK198_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF39159.1"
                     /translation="MQPAIFFLLLFFTAQLHAQSLIERFDDGDFTNNPTWTGQTSLFA
                     VTNGELQLRDLAPPANGTAYLAVAAPTGLSQATTWEFYVRLAFAPSTTNFARVYLSSN
                     SPQLDGSLNGYFIRIGGISGDGDAVELFRQTGTATTLLISGQAGAVGPEPAIARVRIT
                     RNTVGEWSMMADYTGGTNFISQGTPVTDSTHPAGSFAGVYCRYTATRAQAFFFDDIII
                     DPLFFDTTPPVLNSAVAESATSILLSFDEPLETAAAENAGNYLINNGIGQPASAQLMA
                     NSTQVSLQLAAPLVNLQTYQVTATGIRDAAGNTAGAQSAGFTFYNVQPAQPGDLIVTE
                     FMP"
     assembly_gap    4320..4385
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4515..>5377
                     /locus_tag="PK198_10250"
     CDS             4515..>5377
                     /locus_tag="PK198_10250"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRF39160.1"
                     /translation="MKVAVVGITGLVGGVMCKVLEERNFPITEFLAVASERSVGKEVE
                     FKGKTYTVISMEAAVEARPDIAIFSAGGSTSLEWAPKFAAVGTTVVDNSSAWRMHPDK
                     KLVVPEINAGVLTPADKIIANPNCSTIQMVMALAPIHQKYGIKRLVISTYQSVTGTGM
                     KAVKQYHLESKGFEPKPEEMAYFYPIFENCIPHCDVFLDNDYTREEMKLVHETRKILS
                     DDSIRITSTAVRVPVLGGHSESVNVELAKAFEVEDIKQLLREMPGIIVTDDVQNKQYP
                     MPLHAKDRDEV"
     assembly_gap    5378..5387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5652..>6680
                     /locus_tag="PK198_10255"
     CDS             5652..>6680
                     /locus_tag="PK198_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39161.1"
                     /translation="MKNKIVLWGTNDQDERVLIALELKPHANKVEIMTFPESIATEEF
                     SRKMMDEWRDNKPFEVPEGYTLLERELTVADSILPDHLKVERSDVIHRAQTEWHFAVL
                     SAKLHETYKSELNDIKERVEQLSNFDSAAWDHLKTFWDKVQEQVKERNLFRDQADSLR
                     DTANGLFTKLKEMRTRIDNDFKTKSKDNLENFQKLIEDVEARAAKGLRIQPLFDELKE
                     LQSKFKSLEFTRDDRNKVWNRIDEAFKNIKAKRSGGGGGGAQQGGGGGGDNAGERLER
                     RYNGLIGAIEKMQDSVRRDRQELDFQNKKVASSEGQLEAQIRQAKIKMIEERLISKEE
                     KLKEMLDTQ"
     assembly_gap    6681..6780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6781..7402
                     /locus_tag="PK198_10260"
     CDS             <6781..7402
                     /locus_tag="PK198_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39162.1"
                     /translation="KIREGMQVAAQAREEDSEKLEKAAEAIQKRKEKQPQSFFEAAGT
                     MLGDALMDALDTAKAVAEVAGEKIEEAISEIKEEAAEMKEKAAEKVAELKEEAAELKE
                     ELAEKAAEMKEKAAEKVAELKEEAAEKAAELKEDAAEIADAVKESAAELKAIVEEKID
                     AATAAVEVEAAHDDPASTDEPETGIEAQDTPPSAPEAETAPEEEEK"
     gene            complement(7475..8530)
                     /locus_tag="PK198_10265"
     CDS             complement(7475..8530)
                     /locus_tag="PK198_10265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF39163.1"
                     /translation="MSTTKEQIGRLEDKVIYRQYEKDEEIVRFVESVTWGVGETQYEH
                     YYALHRLEHLADGIYFSARQADTDELMGVVIFGRRRILGVRAYYIRFFAVSPKIRGQR
                     ITTPLAMHFFDYLRRTETEPCVFYASTDRTNPGVNRMVGKLGFEEVTFNRTMAFSRFS
                     PKPSRAVSALSEAEFAAFLPRLEAMYVGHGFWTADNVGKDDSYFVLRDDSGEIVAGLQ
                     AYKGFWRIEKMPGFMGKVVLPLVPYTPLRVIFNPKAFHFIAFEGIYMAPGHEHRLAEL
                     MEAVLHHFQMHSAMFWFNIQDPLADKILKNNNLGLLNLFVKNTLARFVASFHHVDPAI
                     EAKLRKRPVYNSSLDYI"
     gene            complement(8541..9077)
                     /gene="dcd"
                     /locus_tag="PK198_10270"
     CDS             complement(8541..9077)
                     /gene="dcd"
                     /locus_tag="PK198_10270"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRF39164.1"
                     /translation="MILSDKKIMEGIEAGQIVIEPFDRDCLGTNSYDVHLGKWLACYE
                     DEVLDARRHNKIRHFEIPEEGIVLQPGRLYLGVTQEYTETFNSVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNHWTLEISCTQPVRVYAGMPVGQLIYFAVEGDIENYYHRKRNAK
                     YNERTDKPVESMMWRNVF"
     gene            complement(9112..10029)
                     /locus_tag="PK198_10275"
     CDS             complement(9112..10029)
                     /locus_tag="PK198_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39165.1"
                     /translation="MEKATFTILKKAQLTGHNAAVFALAPGRQPESFFSAAGDGWIAE
                     WNLDAPENGRLVAQVESRIFSLLHLPEHGRLVAGNMDGGLHWIHLDDPDQTRNIAHHQ
                     RGVFDLKQVGDSVFSAGGEGVLTRWSAAEVRSVEGIHLSNRSLRSIDYCPARNELAVG
                     ASDFNIYLLDATSLQIRQTITRAHNNSVFAVRYSPDARFLLSGGRDAHLRVWDIGQGF
                     ALVSEQPAHWYTINSIAFHPEGRLFATASRDRTIKLWDASNFQLIKVLDTIRSGCHIN
                     SVNALLWLPHRNTLVSASDDRSMILWETE"
     gene            complement(10073..>10341)
                     /locus_tag="PK198_10280"
     CDS             complement(10073..>10341)
                     /locus_tag="PK198_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39166.1"
                     /translation="EVAQYNLASPNDGVQVQQDTAGLLSVQFLQTGNWWWRGGIGALP
                     YEKQGYRFEPGDGKYTLHLDSIPAGSVFLIQKGSSWEEVDVLLK"
     assembly_gap    10342..10351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10352..>11296)
                     /locus_tag="PK198_10285"
     CDS             complement(<10352..>11296)
                     /locus_tag="PK198_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39167.1"
                     /translation="RVCLNFLLSGFFVLTSLLFALRWFDSGERRHWWGAQLVLVAALF
                     TFELGLVIPALSTLLLWLLPGEKGSFFKRFGALAGVQWAVVGMYFLLNKMVLGAWVGH
                     YGEAVHLRFVPAELAANFWRYTAKLALFGRYLGLPEKQAVFGVLDKPVWFWSLSVLLA
                     GALVASWLLRDRLSARLRLTVFFFTAFGLALAPIINLYFNNLMRIDGDRYVYLASMFF
                     WAFAATALFSLPRKWAVVISASILLISVRLLFKTNQRWVESTRIYYALLDNYRWQNEP
                     HVFLLNLPDNYQGAPMFRDHSMKNRALADPLYYLAKKKP"
BASE COUNT         2812 a         2915 c         3030 g         2248 t
ORIGIN      
        1 cggctggtgc catacacttt gtatccgttt cgatggaggt attccgccag tgccttgccc
       61 agacccgatg aggcgccggt gacgagaact actttttcgg gcatttttgg attatttttt
      121 tggtaaatgg ttggatgtca gctacaagct gcaggacgca agcctcaagt cgttgtattt
      181 tgaatatgta gaaaagcagc gcccatcatt tcgatcaaat ctccggcaat gagcgcctcc
      241 tgtccttttt gcttcgccgc caaatcaccg gccaagccat gcaaatacac gcccaacacg
      301 gccgcatcaa gtgccgcata accctgtgcc cgcaagcccg tgatgatgcc ggtgagtacg
      361 tcgccgctgc cgccnnnnnn nnnnnnnnnn nnnnatgccg ggattgcccg ttgcattgaa
      421 ataacaatgg ccgtcgggcg aagcaatgca agaaaacgct cccttcaaaa cgatgacgac
      481 gtgcagttca gccgcttttt ttcgcaagat ttccaatcgc tcgaaaccgt tgttcgaggg
      541 gccaaacata cgctcaaatt cctttggatg cggcgtcagg atggtatttt cgggcaataa
      601 tttcatccat tcgggatgca gggccagcaa attgagcgcg tctgcatcga gtaccaaagg
      661 ctgaacggcg cattccaaaa gctgatgtaa agcattggct gtcaactctt cctgtcccca
      721 gccgcagcct gcgccaatgg ccgaaaacgg actcaaatcg ggcatttcgg aaatatgcag
      781 ttcgtgcgcg tccgccaggg tcatggcctc cggggccgcc gtttgcagga tgggatatgc
      841 gcaacgcgga gtatgcaccg tgaccaaacc cgcgccgctg cgcaaacagg ctcttgtggc
      901 taaaaccgcc gcccccgctt tgccataact gcccgcacaa atgagcgcat ggccgaacac
      961 gcccttgtgg tcgaatcggc ggcgggtttt cagcatattg cgcgccgacg ctgcctctac
     1021 aaaatgaaaa agggtgctta tttccgacaa aaaaccggca tcaaggccga tgctcccgaa
     1081 gcgccactcc cctactctct gttgattatc aggcaaaaag aaagccaatt tgggcgtttc
     1141 aaaactaaag gtgtaatgcg cctccagcac cgcgccctcc gaaggcccat cggtaaacaa
     1201 gccggaaggc acgtctatgg ccacccgcgt gagagactgt tcattgcaat ggcgaatcaa
     1261 atcggcccag tagcctgtta ccggcctgct gagtccggaa ccgaacaggg catctatgag
     1321 caaggcgcct tgtggccaat ccggcagcgg gtcgccggcg tgcagatgat gcactgcaat
     1381 ggcgcctccc tcggtgggca agcggtccca attgatctga aaatcaggcg aaaccgccgc
     1441 gccgatgtga caacgccaca cagctacatc gtaaaaacgg cgatgcaaaa ggcgcgctac
     1501 ggccaatccg tcacctccgt tgttgccggg tccgaccaaa atgagcacct ttctgtctga
     1561 gtcgggaaat ggctgcacca accatttgac aaaagtcaga gaggcgcgct ccatcaggtc
     1621 aatggatgca atcggctcat tggcaatggt gtaggcgtcg agtgcgcgag tttgttcggc
     1681 gctgagaatt ttcatggttg ctctttattt ctaatacccc gccagcacac acgtcttctt
     1741 cacccgttcc acctgcccgt cgggatgaaa caactctatc cacaggatgt aaatgccggt
     1801 gcgggctttt gtgccctcgt cgttggcgcc gtcccatttg aaaacgccct ccgaaccaag
     1861 cagttcgctg cgcaaaaggg tgcgtacggt gcgaccggcg gcgtcgaaaa tgagcaggtt
     1921 gagcagaaag ccgggtttat cggcggtgta aaaaatttgc aacacgtctt catatccgtc
     1981 gccgtcgggc gagaaggttt tgttgggcag agaaatcatc tcatcggcca gagaggggct
     2041 gatgcggaat tgcgaatttc ggtagccggg cgtggcaaaa ccgactgcca ctgctgcgga
     2101 atgccagttg ccggcgctgt tggaaggccc cgcagagttg atgcgctcca aagaaacgcc
     2161 atctttggta tccaaaagcg gggaatgcca ggcttcggaa taatcgaacg catctatcag
     2221 cacgccgtta aaaagcaggg ttgcattgcc ggacgcatca tcgaagccgg gcagtgcatt
     2281 ttgtaccaaa gcgctgatat tcggcacatt gtaataggct tgtatgttgg caatatcgct
     2341 ggaaatggct gcgtactcat cgggaaagag caggaagtca gactgaaaag tagtggaaaa
     2401 attagtcgtt cgctgtgtgt tttgaatttg caaacccctc agattcaaaa tcttggccga
     2461 agtgttgtac agttctataa aatcctgccc gccggtgcgc ggattgaaca acaactcatt
     2521 gataaccaga tcaccgggag tcatggcttc cgccaatccg atgcgcgtac tcatggctgc
     2581 tccggccgga tttccgaggc aatcccgaac atcggcgacn nnnnnnnnng tgagcgtgta
     2641 ggaacgcccg gccatgagcg gggccgacaa ggcgagcaaa acctgcgtag caggcgtgag
     2701 caaggtggcg gagatcaccc ccacgccgtc gctgaacgag aaccaaccgg cattgagcga
     2761 ggactcggaa agcggttcat cgaaagtgac gatgactgaa gaaggcgtgg acaaggcggc
     2821 ccggagcagt cttggcccga tgttgtcgtt gggcgtgcct tgcaccgaat tgagctgccc
     2881 cggtgtgccg ccgcgcgggt gtacggaagc gcgccagttg gccgggcagt tgtcggccag
     2941 attgttgccg atgcgctcta tgctccatcc accgtcggtt ttggcggggt cctgatacca
     3001 actcaggtca tattccaaat aaaacaactc ttcgccctgc gcattgctga ggataatttc
     3061 atcgccgctg ttggtcagtg cggggaagga agtcatgccg gccacattgc cgaaaggcgc
     3121 aaaagctgcg atgttgtttt gggggcacac aatgacgtat tggccgggca aaagtaaaaa
     3181 gttgggcaaa ggctgcggcg aactgccgtt ggagtatcta atatcttgta attgnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnng gcataaattc cgtcacaatc agatcgccgg gctgagcggg ctgaacattg
     3361 taaaaagtaa aacctgccga ttgcgcgcct gcggtattgc ccgctgcgtc gcggatgccg
     3421 gttgccgtta cctgataggt ttgcaaattg accaaaggag ccgccaattg caagctgact
     3481 tgcgtactat tggccatgag ttgcgcagaa gccggttgac ctattccatt gttgatgaga
     3541 tagttgcccg cattttcggc agcagcggtt tcaagcggtt cgtcaaaact gagtagtatg
     3601 gaggttgcac tttccgcaac ggcgctgttc aaaacaggcg gcgtggtatc gaaaaaaagt
     3661 gggtctatga tgatgtcgtc gaagaaaaaa gcctgcgccc gcgtggccgt atagcggcaa
     3721 tagacccctg caaaagagcc tgccggatgc gtagaatctg tgacgggagt accctgagag
     3781 atgaaatttg tgccgccggt gtaatcggcc atcatactcc actcgccgac cgtgttgcgg
     3841 gtgatgcgca cgcgtgcaat ggccggttcg gggcctaccg cgccggcctg cccgctgatg
     3901 agcaaggtgg tagcagttcc cgtttggcgg aacagttcca ccgcgtcgcc gtcgccgctg
     3961 atgccgccga tgcgaatgaa atagccgttg agcgaaccgt ccaattgggg cgagttggaa
     4021 ctgagataca ccctggcgaa attggtggtg gagggcgcaa aggccagccg gacatagaat
     4081 tcccaagtgg tagcctgaga caggccggtg ggagcggcta cggccagata ggcggttccg
     4141 ttggcaggcg gcgcgaggtc gcggagttgc agttcgccgt tggtcacggc aaacagagaa
     4201 gtttggccgg tccaggtcgg gttgttggta aaatcgccgt catcaaagcg ctcgatgagg
     4261 ctttgtgcat ggagttgcgc ggtgaagaag agcagcagaa agaaaattgc aggttgcatn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnatagg acacgcgagg tagaacctcg cgcgagcacg gtgaaaaaat gacaggtttc
     4441 atggcgacga aaatagcaac aaactcggat tgcgtatatc tttgcgcctc ttttgaaaac
     4501 aaaattaacc tgaaatgaaa gtagccgttg ttggcatcac cggtttggtc ggtggcgtga
     4561 tgtgcaaagt actggaagaa cgcaattttc ccatcaccga atttttggcc gtagcctccg
     4621 agcgttcggt gggcaaagag gtagaattta aaggcaaaac ctacacggtc atcagcatgg
     4681 aagccgccgt ggaagcccgc cccgacatag ccattttttc cgccggaggc agcacctccc
     4741 tcgaatgggc gcccaaattt gccgccgtcg gtaccacggt agtggacaac tcctcggcct
     4801 ggcgcatgca ccccgataaa aaactcgtgg tgcccgaaat caacgccggc gtactcactc
     4861 ctgccgataa aatcatcgcc aaccccaact gttctaccat ccagatggtg atggcgctgg
     4921 cgccgattca tcaaaaatac ggcattaagc gcttggttat cagtacctac caatctgtta
     4981 ccggcaccgg tatgaaagcc gtgaagcagt atcatttaga gagcaaaggt tttgaaccca
     5041 agccggagga aatggcctat ttctatccca tttttgagaa ctgcattccg cattgcgatg
     5101 tgtttttgga caacgactac acccgcgagg aaatgaagct ggtacacgaa acgcgcaaaa
     5161 tcctcagcga cgactccatt cgcatcactt ccactgctgt gcgcgtgccg gtgttgggcg
     5221 gccacagcga gtcagtcaat gtggaattag caaaggcgtt tgaagtggaa gacatcaaac
     5281 aactgctgcg cgaaatgccg ggcatcatcg tcaccgacga cgtccagaac aagcagtacc
     5341 ccatgccttt gcacgccaaa gaccgcgatg aggtgttnnn nnnnnnncat tcgccgcgat
     5401 gaatcggtgg acaacggcct caatatgtgg atcgtatctg acaatctgcg caaaggcgcg
     5461 gccaccaatg ccgtccaaat cgcagagtat ttggctgccc ataaattggt gggtggatag
     5521 cacgcatttt tgttttgttg tacaaaaaac agtaactttg gctgttcaat ggagcatacc
     5581 gacggacaac cgccgatact cattacagtg aatcgtaaat ttcgtaattg ctaactttag
     5641 tactgactga catgaagaac aagattgtac tttggggtac caacgatcag gatgaacgcg
     5701 tactcattgc gctggaactc aagccgcacg ccaacaaggt cgaaatcatg accttcccgg
     5761 aaagcatcgc caccgaagaa ttcagccgca aaatgatgga tgaatggcgc gacaacaagc
     5821 cattcgaagt gccggaaggc tacaccctcc tcgaacggga actgaccgta gccgacagca
     5881 tcctgcccga ccatctcaag gtggagcgct ccgatgtcat ccaccgcgcc caaacagagt
     5941 ggcacttcgc ggtgctttcg gccaaacttc acgaaactta caaatcggaa ctcaacgaca
     6001 taaaagagcg cgtggagcaa ctcagcaatt ttgactccgc cgcttgggac catctcaaaa
     6061 ccttctggga caaagtgcag gagcaggtca aagagcgcaa cctcttccgc gatcaggccg
     6121 atagcctccg cgacactgcc aacgggctgt tcaccaagct caaggagatg cgcacccgta
     6181 tagacaacga cttcaaaacc aagtcgaaag acaatctgga gaacttccaa aaactcatcg
     6241 aagacgtgga agcccgcgcc gccaaaggtc tgcgcattca gcctttgttc gacgaactga
     6301 aggaactgca aagcaagttc aagagcctcg aatttacccg cgacgaccgc aataaggtat
     6361 ggaatcgcat tgacgaggca ttcaaaaaca tcaaagccaa gcgctccggc ggtggcggcg
     6421 gtggcgctca acaaggcgga ggaggcggcg gagacaatgc cggcgaacgc ctcgaacgcc
     6481 gctacaatgg attgataggc gccatcgaaa aaatgcagga ctctgttcgc agggatcgtc
     6541 aggagttgga cttccaaaac aaaaaagtag cctcctctga gggacaatta gaagctcaaa
     6601 tccgtcaggc caagatcaaa atgatcgaag agcgcctcat ttccaaagag gaaaaactca
     6661 aagaaatgct ggatacgcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 gaaaatccgt gaaggcatgc aggtggctgc acaagcacgc gaggaggatt ctgaaaaatt
     6841 ggaaaaagcc gccgaagcca ttcagaaacg caaagaaaaa caaccgcaat ccttctttga
     6901 agctgccggc accatgctgg gcgatgctct gatggatgcg cttgatactg caaaagcagt
     6961 ggccgaagtg gctggagaaa aaatcgaaga ggccatcagc gagatcaaag aagaagccgc
     7021 cgaaatgaag gagaaagctg ccgaaaaagt ggctgaactg aaggaagagg ctgccgaact
     7081 caaagaggaa ttggccgaaa aggctgccga aatgaaggag aaagctgctg aaaaagtggc
     7141 cgaactgaag gaagaggctg ccgagaaagc cgccgaactg aaagaggatg ccgccgaaat
     7201 agcagatgct gtgaaggaaa gtgccgctga actgaaagcc atcgtggagg aaaaaataga
     7261 tgccgctacc gccgccgtgg aagtggaagc tgctcacgat gacccggcaa gcaccgatga
     7321 accggaaacg ggcatcgaag ctcaggacac gccgccctca gcgccggaag ctgaaacagc
     7381 gccggaggaa gaggaaaagt aattgtttag aatggacgga agtctcaaca gcccgcgctt
     7441 ggaacatcta attcttggtt cgggctgttt ttttctaaat ataatccaac gaagaattat
     7501 aaaccggccg cttccgcaat ttggcctcaa tagcgggatc aacatgatga aatgaggcca
     7561 caaaacgcgc cagcgtgttt tttacaaaaa gattgagcaa acccagattg ttgtttttca
     7621 atattttatc agcgagcgga tcctggatat tgaaccaaaa catcgccgag tgcatctgaa
     7681 aatgatgcaa caccgcctcc atcaactctg ctaaacgatg ctcatgcccc ggcgccatgt
     7741 aaatgccttc aaacgcaatg aaatgaaatg ccttggggtt aaagatgacg cgcaaaggtg
     7801 tgtagggtac taaaggcaga acgaccttgc ccatgaagcc cggcatcttc tcaatacgcc
     7861 aaaacccctt gtacgcctgc aatcccgcca cgatctcgcc cgaatcgtcg cgcaacacaa
     7921 aatagctgtc gtctttgccc acattatcgg ccgtccagaa gccatgtccg acatacatcg
     7981 cctccaaacg aggcaaaaac gccgcaaatt ccgcctcaga caaggccgac accgcccggc
     8041 tgggctttgg tgaaaaccga ctgaaggcca tggtgcggtt gaatgtgact tcttcaaaac
     8101 ccagcttgcc caccatccga ttgacgccgg ggttggtacg gtcggtagaa gcgtaaaaaa
     8161 cgcagggttc cgtttctgtg cgcctcagat aatcaaagaa gtgcatggcg agcggcgtag
     8221 tgatgcgctg ccctctgatc ttgggcgaaa cggcaaaaaa gcgaatgtaa tatgcccgga
     8281 cgccgagtat gcgccgcctg ccgaaaatga ccacgcccat caattcatcg gtgtcggcct
     8341 ggcgggcgct gaagtaaatg ccatcggcca aatgctccaa ccggtgcagg gcgtagtagt
     8401 gctcgtattg ggtttcgccg acgccccagg tcacgctttc cacaaagcgc acaatttcct
     8461 catctttctc gtactggcgg tagatgactt tatcttcgag ccggcctatt tgttctttgg
     8521 tggtggacat aggtcgttat ttaaaaaaca ttcctccaca tcatactttc cactggtttg
     8581 tcggtgcgct cattgtactt ggcattgcgt ttgcggtggt aataattttc aatatcgccc
     8641 tccacagcaa aataaatgag ctgaccgact ggcattccgg cataaacccg cacgggttga
     8701 gtacaggaaa tttcgagcgt ccagtggttg caaaaaccca cgtcaccctt gcccgcagtg
     8761 gcatgtatgt caataccgag gcgccccacg ctggatttgc cctctaaaaa aggcaccgaa
     8821 ttgaaggttt ccgtgtattc ctgcgtgacg ccgaggtaga gcctgccggg ttgcagcaca
     8881 atgccttctt cagggatttc aaaatggcgg attttgttgt gtcggcgggc atccagcacc
     8941 tcgtcttcgt agcaggccag ccatttgccg agatgtacgt cgtaggaatt ggtacccaga
     9001 caatcgcggt cgaagggttc gatgacgatc tgccccgcct caatgccttc cattattttt
     9061 ttatcgctca gaatcatgca ggaaaaattt gaacgggcaa aggtagcggt ttcattcggt
     9121 ttcccacaaa atcatactcc ggtcgtcgct ggccgacacg agggtgttgc gatgaggcag
     9181 ccagagcagc gcattgacgg agttgatgtg gcaaccgctg cggatggtgt ctaatacttt
     9241 gattagctga aaattggagg catcccaaag tttgatggtc cggtcgcggc tggcagtggc
     9301 aaagagcctg ccttcggggt gaaaagcaat gctgttgatg gtgtaccaat gggccggctg
     9361 ttcggaaacg agcgcaaaac cctgcccgat gtcccacacg cgcaggtggg cgtcgcggcc
     9421 gccggagagc agaaagcgcg catcggggct gtaccggacg gcaaaaacgg aattgttgtg
     9481 cgcccgggtt atggtttgcc gaatttgcag gctagtggca tccaacaaat agatattgaa
     9541 atcgctggcg ccgacggcca attcgttgcg ggccgggcaa tagtctatac tgcgcaaact
     9601 cctgttgctc aagtgaatac cctctacgga gcgcacctcg gcagccgacc agcgggtgag
     9661 tacgccctcg ccgcccgcgc tgaatacgga gtcgcccact tgtttgaggt caaaaacccc
     9721 gcgttgatga tgcgcgatgt tgcgggtttg atcggggtca tcgagatgaa tccagtgcaa
     9781 gcctccgtcc atgttcccgg caacaagtcg gccatgttcg ggcaagtgca ggagcgaaaa
     9841 aatacgcgat tctacctgtg ctacgaggcg gccgttttcg ggggcgtcca gattccattc
     9901 ggctatccag ccgtcgccgg cggcagagaa gaaggattcg ggttgccgtc cgggcgccag
     9961 ggcgaacaca gcagcattgt gtccggtgag ctgggccttt ttgagtatgg tgaaagttgc
    10021 tttttccatc tcgaacgaaa cggtcggcgc cgcaattggt tctgccgagc atctatttta
    10081 aaagcacgtc cacctcttcc caactgctgc ctttttgaat caaaaagacc gagcctgccg
    10141 gaatcgaatc taagtggagc gtgtatttcc catcgccggg ttcaaagcgg tagccctgtt
    10201 tttcgtaagg caaagccccg ataccgccgc gccaccacca gttgcctgtt tgcagaaact
    10261 gtacagacag caggccggca gtatcttgtt gtacctgcac gccgtcattg ggcgaagcga
    10321 ggttgtattg ggccacctca tnnnnnnnnn ncggtttttt ctttgccaaa taatacaaag
    10381 gatcggccaa tgcacggttt ttcatcgagt gatcgcggaa catgggagcg ccctggtagt
    10441 tgtcgggcag attgagcagg aaaacatgcg gctcgttttg ccaacgatag ttatccaaca
    10501 gagcgtagta aatacgggtg ctttctaccc agcgttggtt ggttttgaac agcagtctta
    10561 ccgaaatgag caagatgctc gctgaaatga cgactgccca tttgcgtggc agcgaaaaga
    10621 gcgccgttgc tgcaaaagcc cagaaaaaca tggaggcgag ataaacgtaa cggtcgccgt
    10681 caatgcgcat caggttgttg aaatacaggt tgatgatggg ggccaatgcc agtccgaatg
    10741 ccgtgaaaaa aaacaccgtg aggcgcaggc gggccgacag gcggtcgcgc agcagccagg
    10801 aagctacaag cgcgccggca agcaaaacgc tgagcgacca gaaccaaacc ggcttgtcca
    10861 atacaccgaa taccgcctgt ttttccggca ggcccaaata gcgaccaaac agagctaatt
    10921 tggccgtgta gcgccagaaa ttggcggcca gttcagccgg cacaaaacgc agatgcaccg
    10981 cctctccata gtgacctacc caggcgccca ataccatttt attgagcaaa aaatacatgc
    11041 cgacaaccgc ccactgtacg ccggccaatg ctccgaaacg cttgaaaaaa ctgccttttt
    11101 cgcccggcaa caaccacaat aacagcgtac tcagtgccgg aatgaccaag cccaactcga
    11161 acgtaaacag ggccgctacc aaaaccaact gagcgcccca ccagtgccgc cgctcgccac
    11221 tgtcgaacca ccgaagagcg aagagcagcg aagtcaacac aaaaaagccc gacaagagga
    11281 agttgagaca aacccg
//
LOCUS       DAORLF010000106        11189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3120_length_11189_cov_1.988953, whole genome shotgun sequence.
ACCESSION   DAORLF010000106 DAORLF010000000
VERSION     DAORLF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_11189_cov_1.988953"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>655
                     /locus_tag="PK198_10290"
     CDS             <1..>655
                     /locus_tag="PK198_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39168.1"
                     /translation="PVKWSEYKAALPEWFDYVILRQGKQADISEVNPYKDQVTMNLTR
                     DQSQRSEIITVPVTMREYRTAMKDVPAMKMEAFVTTEDDYLARIRFQLKTVTWPQSPP
                     QQILTNWPDVAKRLMEHESFGLQFSKKGNYSKVWESAQLLLNDAQSVKEKADKLYRFV
                     GGVMTWDEDFGIYTRNSLNQCFEQKKGSAAETNLMLLALFKEAGITAYPALTSTRDHG
                     "
     assembly_gap    656..677
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <678..1503
                     /locus_tag="PK198_10295"
     CDS             <678..1503
                     /locus_tag="PK198_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39169.1"
                     /translation="FELYPFISQFNHVMILADIEGQFKIFDVGSIYRPAGFPRISALN
                     GQAWVVIPGKPEWVGIQAPNASSTLVYKSTLDEDGGLSGRFRMKMDGYDAVPHREGLH
                     KEKTAEYWQKKLAEDFPDVSVESASITNLDTLSASLNADLQCSFSGAAQNNGDLMYLA
                     PVLMPPFKENPFKLAQRNYPVEIPYPISYQFVLQLDVPATYEVDELPENLNMELPERG
                     GSFVYQVSRNENTLNINYKLRLDQLEFDPEEYAGLKMFFDQLIAKQEEQIVLKKKD"
     gene            complement(1574..2029)
                     /locus_tag="PK198_10300"
     CDS             complement(1574..2029)
                     /locus_tag="PK198_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39170.1"
                     /translation="MKITFKQINVLYLALLAGQIMFAVTVLYVGGVPKSLGKLSFDDP
                     MLLMGIAVTFFSIFAAYGINEKRKTAGAQLSDFQEKIEHYRGVVIVRCALTEGANLMA
                     LVMGMLSKQGFFFVLFVVGLLAFLYFRPSVQEFSRSYHLRPEEERDLYE"
     gene            complement(2041..4857)
                     /locus_tag="PK198_10305"
     CDS             complement(2041..4857)
                     /locus_tag="PK198_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF39171.1"
                     /translation="MEYSPRDIEQKWKSIWEQKQTYRTENGGSRPKYYILDMFPYPSG
                     AGLHVGHPLGYIASDIFARYKRLKGFNVLHPMGYDSFGLPAEQYAIQTGIHPAIATHE
                     NIDRYRSQLENLGFSFDWSREVRTSSPEYYRWTQWIFQQLFKHYFDKEAGKALPIDTL
                     VQRLASEGNARIQAACEENTPLISASDWNAMSEREQQLFLLNYRLTFPEESYVNWCPA
                     MGTVLSNDEVKDGLSERGGHPVERKLMAQWSMRITAYADRLIDGLEEIDWPLSLKEQQ
                     RNWIGRSQGASVKFPLENGDKQIEVFTTRVDTIYGVTFMVLAPEHEWVDELTTDAHRE
                     EINAYRTWTASRSEVERMAEVKKVTGAFTGAYCVNPFNGAKVPIYIADYVLAGYGTGA
                     VMAVPSGDQRDWNFATHFQLPIIPILDAQQDMDKGADPTKEGRYINSGIINGMSYEEA
                     VPALIQWLEERGLGKGKVQYRLRNAVFSRQRYWGEPVPAYWKDDVPYLVDENELPLVL
                     PDVDKYLPTETGEPPLGRAENWKYQGKYEYELSTMPGWAGSSWYWYRYMDPHNEKGFV
                     SKEAVDYWQDVDLYVGGSEHAVGHLLYSRFWNHFLYDLGLVPKREYAKKLINQGMIQG
                     VIEFIYLIKPEDGDDSRTFVSADLLGNYPGKEFALIPVHVDFVKDYGRDESHLDAAGI
                     EQFKEWRPDFKAAAFVTNDKGQLLTKSEVGKMSKRYFNVVNPDDVVARYGADCFRIYE
                     MFLGPIEQAKPWNTNSISGVSRFLNNFWRLFFRDGKWLVNDETPTKEELKVLHTAIKK
                     VSEDVERFSFNTCVAAFMVAGNDLNKLQCHKRAILEPLLVMMAPFAVHLTEELWEHLG
                     HTGSIHHADWPELDEQWLVEDEITYPISVNGKLRLAVPFPASAQPAELEKAALEMEGV
                     QRWLEGKPVRKVIVVPGRMINIVV"
     gene            5022..5720
                     /locus_tag="PK198_10310"
     CDS             5022..5720
                     /locus_tag="PK198_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39172.1"
                     /translation="MATRKKSSAKADETLVDIVEVKEQAEDFFERYRFVILGAVGALV
                     LAVGGFFAYTNFYKIPKQQEAVEQMYQAQIQFERDSFTQALTNPGGGYQGFLDISENY
                     KGTDAANLALYYAGISYLNLGQYDAAIDYLKSYKPAGDVIPAMKFGALGDAYSEKNDF
                     GQAMSYYKKAASTGVNEAITPYYLKKIGMLHERNSEWDQAIKVYQEIKDKYPTSPDGR
                     DIDKYLIRAEAKKG"
     gene            5729..6082
                     /locus_tag="PK198_10315"
     CDS             5729..6082
                     /locus_tag="PK198_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRF39173.1"
                     /translation="MNDQVVKSSSSIHIRVGLNEENYPVQIEWHSDDAPDAIQPCKAM
                     LLSLFDEENKETVKIDLWTNEMQVMEMDRFFFQTLRGLADTYHKATRNARLASAMQQF
                     VQYFGEETEIIPREK"
     gene            complement(6105..7892)
                     /locus_tag="PK198_10320"
     CDS             complement(6105..7892)
                     /locus_tag="PK198_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF39174.1"
                     /translation="MNKCTTLHFLLTFLLLSSQAKAQTDSLQQALSGMTEREQVEYIG
                     KHFSQIASSNFSTAIQTGEKALALARKNRWPQWEASLLKNIGIAYYLKGDYETCLPLY
                     QQALDLYEQIGDQAGRGETLKELGNYFKRLKRYDKALQQLEQAAQLCSGARDTNCLTA
                     ALDIKAVVLIEQGRLDDAKEVLLYEQALLERNGNDRALSYTLSNLADVAIGQKRFDDA
                     AALLARSTDIRQRMGDAAGVAINTNNTGEMYLHSGQADKAKPYFEKTIEMSSAIGFTD
                     LQRHAMQMLAETCSALSLHQEAMDWLNRSYALKDSIFNLEHSRQIAEMAEKYEVEKKE
                     KELAQQQKQLQRRSFQLALAAAGLAILALVSGFVFHRQRLKQHQLRREAELKTELIKS
                     ESALRLQRERLRISRDLHDNLGAELTIIGSSLARNITQADNDIQKKSLETIRNNARQA
                     MSQLRETIWAIRYEAFSLSDLADKVSDFALRAGAPPLAVLVSAGADLTLSPAQTLNLF
                     RIAQEAINNSIKYSGATEMSLAFEVPTENQLLMRISDNGCGFDAGQRANSGNGLANIK
                     IRAEELGGGSEIISAAAVGTTVEVRIPVL"
     gene            complement(8199..9587)
                     /locus_tag="PK198_10325"
     CDS             complement(8199..9587)
                     /locus_tag="PK198_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39175.1"
                     /translation="MMSSFLSLRRLGWLALLALLALAAVFFRERALFTDMAFQTVLMS
                     TEGDFQIMVNRFGVVLVQALPLLAIKMGLPLQAVSLLYSISFPLLFLGVYALMVRVCK
                     QEELGLSLVTLFTLMVFDAFYWPSSEQQQGLAFLLLYFAFAQRDLRFKSISAILVALI
                     AVPVLAWYHPLIFIPFYFLWAFFLLRQAEVRNGRYLALGGYMASVLVFKSLFSGNWYD
                     DDKFRIFKENLAALFPDYFSMASHGQFAQWCLAHWYFLPLLWLAVSGFYLHRRQWKPL
                     LLMWLFGLGHLMLLHIASPEHPYRFYAEVNCLPLTLYAALPFWYDIAPTIRPKHWLTA
                     GFALFLILRIAAIGLHHKPWTQRLERLESILAEGRQNTGSSRYLVRETPALRDSLFIT
                     WSAPYETLLLSAMQHPDSAQTLLIRSDFGKFEKEIKEPGWLLSDFGAKPDSTLNKRYF
                     RLPEGRYEFVRE"
     gene            complement(9608..>10229)
                     /locus_tag="PK198_10330"
     CDS             complement(9608..>10229)
                     /locus_tag="PK198_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39176.1"
                     /translation="DEGYMRKAYELCRNNNVLLIADEVQTGIGRTGKLLACDHDGFRP
                     DILVLGKALGGGVFPVSAALARNEIMLTIKPGEHGSTFGGNPLACAVAMASLEVVKDE
                     QLAENAEYLGQIFRQRLSDNLMRRTELVTGVRGKGLLNAIIIADDEDGHTAWDICMQM
                     AENGLLAKPTHGNIIRFAPPLVMTEDQMHECCDIIEQTILHYQVPE"
     assembly_gap    10230..10318
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10319..10859)
                     /locus_tag="PK198_10335"
     CDS             complement(<10319..10859)
                     /locus_tag="PK198_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39177.1"
                     /translation="MLQDTIFSSQDLMALEDRYGAHNYHPLPVVLSRGQGVHVWDTEG
                     KQYYDFLSAYSAVNQGHCHPRIISALTEQAQKLTLTSRAFYNDMLGRFEQYITEYFGY
                     DRVLPANTGVEGGETAVKLARKWAYLVKGVPVNQAKIIFAANNFWGRTLAAISSSTDP
                     KSTKDFGPYMPGYVIIPYND"
BASE COUNT         2556 a         2756 c         3094 g         2672 t
ORIGIN      
        1 tcccgtcaaa tggagcgaat acaaggctgc tcttcccgaa tggtttgatt atgtcattct
       61 tcggcagggc aagcaggccg atatttcaga ggtgaatcct tacaaagatc aggtcaccat
      121 gaatttgacg agagatcagt cgcagcgcag cgagattatt acggtgcccg ttaccatgcg
      181 cgagtaccgc acggccatga aagatgtacc cgccatgaaa atggaggctt tcgtgaccac
      241 tgaagacgac tatctggctc ggatcagatt ccagttgaaa accgttacct ggccgcaaag
      301 cccgcctcaa caaatcctaa ccaactggcc ggacgtagcc aaacgcctga tggaacatga
      361 aagctttggt ttacagttca gcaaaaaagg caattactcc aaggtgtggg aaagcgcgca
      421 attgctgctc aacgatgcgc aatccgtgaa ggaaaaagcc gataagttgt accgttttgt
      481 aggaggagtt atgacctggg atgaagattt cggcatttac acccgcaatt ctcttaatca
      541 gtgtttcgag caaaagaaag gctcggccgc cgaaacgaat ctcatgttgc tggcgctttt
      601 taaagaagca ggcatcacgg catatccggc tctgaccagc acccgcgacc acggtnnnnn
      661 nnnnnnnnnn nnnnnnngtt tgaactgtac ccctttattt ctcaattcaa ccatgtgatg
      721 atattggccg acatcgaagg gcagttcaaa atattcgacg taggcagcat atacaggccg
      781 gcgggttttc cgcgcatttc ggcgctcaat gggcaggctt gggtcgtcat tcccggcaag
      841 cctgagtggg taggtattca ggcgcccaac gcctcctcta ccttagttta taaatctacc
      901 ttggatgaag acggcggtct gagcggcaga ttcagaatga aaatggacgg ctacgatgcg
      961 gtaccgcacc gggagggcct gcacaaagaa aaaaccgctg aatattggca gaaaaaattg
     1021 gcggaagact ttcctgatgt gtcggtagag tcggcttcca ttaccaatct cgatacgctt
     1081 tcggcgtcct tgaatgccga cttgcaatgc agtttcagcg gggcggccca aaataacggc
     1141 gatctgatgt atctggcgcc ggtactcatg ccgccgttta aagaaaatcc tttcaaactg
     1201 gcccagcgca actatccggt ggaaatacct tatcccattt cataccagtt tgtgttgcag
     1261 ttggacgtgc ctgccactta cgaagtggac gaacttcccg aaaatctcaa tatggagctg
     1321 ccggagcggg gcggtagttt tgtttatcag gtcagcagaa acgaaaacac gctgaatatc
     1381 aattataaat tgcggttaga ccagttggag ttcgaccctg aagaatatgc cggtctgaag
     1441 atgttcttcg atcaactcat cgcaaaacag gaagagcaga tcgtgctgaa aaagaaggat
     1501 tgagccggtt taggcagggt agggaagggg tggtcgtatg cttggccacc tcttccctac
     1561 catcctcaaa cgattactcg tacaaatccc tttcttcctc cgggcgcaaa tggtaactgc
     1621 gggaaaactc ctgaacggaa ggccggaaat acaaaaaggc cagcaaaccc acaacaaaca
     1681 gtacaaagaa gaagccctgt ttgctcagca tacccatcac caacgccatc agattggcgc
     1741 cttcggtgag cgcgcaacga acgatgacca cgccccggta atgctctatt ttttcttgaa
     1801 aatcagaaag ttgagcaccg gcggttttcc gcttttcatt gatgccgtaa gctgcaaaaa
     1861 tggaaaaaaa ggtaacggcg atgcccatca gcagcatggg gtcgtcaaaa ctgagcttgc
     1921 ccagcgattt gggaacaccg cccacataca gcaccgtaac ggcaaacatg atctgcccgg
     1981 ccaacagggc gaggtacaga acgttgattt gtttgaacgt gattttcatt ggcttcaatt
     2041 ttacaccaca atattaatca ttcggcccgg caccacaatg accttgcgca cgggtttgcc
     2101 ttcgagccag cgctgcacgc cttccatttc gagggcggct ttttccagtt cggcaggctg
     2161 tgcagaagcg gggaagggca cggcaagccg cagtttcccg ttgacgctga tggggtaggt
     2221 gatctcatct tctaccaacc attgctcatc caattccggc caatctgcgt ggtgaatgct
     2281 gccggtatgg cccaggtgtt cccacagttc ctcggtgagg tgtacggcaa agggggccat
     2341 catcaccaag agcggttcta atatggcacg cttgtggcat tgaagtttgt tgaggtcgtt
     2401 gccggccacc ataaatgccg ccacgcaggt gttgaaggaa aaacgctcta catcttccga
     2461 tacctttttg atggcggtgt gcagcacttt cagttcttct ttggtgggcg tttcgtcgtt
     2521 caccaaccac ttgccgtcgc ggaagaacag gcgccagaaa ttgttgagga aacggctcac
     2581 cccgctgatg ctgttggtgt tccagggctt ggcctgctca atggggccga ggaacatctc
     2641 gtaaatgcgg aagcagtcgg cgccgtagcg tgctaccaca tcgtcgggat tgacgacgtt
     2701 gaaatagcgc ttcgacatct tgcccacttc gcttttggta agcaattggc ctttgtcgtt
     2761 ggtaacgaag gcggcggctt tgaaatcggg gcgccattct ttgaactgct caatgccggc
     2821 tgcatccaag tgcgactcgt cgcggccata gtccttcaca aagtccacat gcacggggat
     2881 gagggcaaat tccttgccgg gataattgcc caacaggtcg gcagagacga aggtgcggga
     2941 gtcgtcgccg tcttctggtt tgatgagata aatgaactca atcacccctt gaatcatgcc
     3001 ctgattgatg agctttttgg cgtattcgcg cttgggtacc aatccgaggt cgtacaggaa
     3061 atgattccag aagcggctgt acagcaggtg tcctacggcg tgctcggaac cgcccacata
     3121 caagtccaca tcctgccagt agtccacggc ctctttgctc acgaatccct tctcgttgtg
     3181 ggggtccatg tagcgatacc agtaccagga agagccggcc caaccgggca tggtactcag
     3241 ttcatactcg tatttgccct gatatttcca gttttcagcc cggccgaggg gcggttcgcc
     3301 ggtttcggtg ggcaggtatt tgtccacgtc gggcagcacg agcggcagtt cgttttcgtc
     3361 cacgaggtag ggcacgtcgt ctttccagta ggcgggcacg ggttcgcccc aatagcgctg
     3421 gcgactgaac acggcgttgc ggaggcggta ttgcaccttg cccttgccaa ggccgcgttc
     3481 ttcgagccac tgaatcagag caggaacggc ttcctcgtag ctcatgccgt tgatgatacc
     3541 ggaattgatg taacggccct ctttggtggg atcggcgcct ttgtccatgt cctgttgcgc
     3601 atccagaatg gggatgatgg gcagttggaa atgtgtggca aagttccagt cgcgttggtc
     3661 gccggaaggt accgccatga ccgcgccggt gccgtagccc gccagcacat aatcggcgat
     3721 gtaaatgggt actttggcgc cattgaaagg attgacgcaa taagcgccgg tgaaagcgcc
     3781 ggtcactttt ttcacttcgg ccatgcgttc cacctccgag cgggaagccg tccaggtgcg
     3841 gtaggcgttg atttcctcgc ggtgcgcatc ggtggtcagt tcgtccaccc actcgtgttc
     3901 gggggcaagc accataaaag tgacgccata tatggtgtcc acacgggtag tgaatacttc
     3961 aatctgtttg tcgccatttt ctaatggaaa cttcacactg gctccctgcg agcgacctat
     4021 ccaattgcgt tgctgctctt tgaggctcaa cggccagtct atttcttcta agccatcaat
     4081 gaggcggtcg gcatacgcgg tgatgcgcat gctccactga gccatgagtt tgcgctctac
     4141 gggatggccg cctctttcac tcaagccgtc ttttacctcg tcgtttgaca gcaccgttcc
     4201 catcgcgggg caccagttga cgtagctttc ttcggggaag gtgaggcggt agttgagcag
     4261 gaacaattgc tgttctcgct cgctcatggc gttccagtcg ctggcgctga tgaggggcgt
     4321 attttcctcg caggcggcct gtatgcgggc attgccttcg gaggccaagc gctgcaccag
     4381 agtgtctatg ggcagggcct tgccggcttc tttatcgaaa tagtgtttga aaagttgctg
     4441 gaaaatccac tgtgtccagc gatagtattc cgggctgctg gtgcgtacct cgcggctcca
     4501 gtcgaaggaa aaaccgagat tttccaactg gctgcggtag cggtcaatgt tttcatgggt
     4561 agcaatggca ggatggatgc ccgtttggat ggcgtattgt tctgccggaa ggccaaagga
     4621 gtcgtagccc ataggatgca gtacattgaa tcctttgagt cgcttgtatc gggcgaagat
     4681 atcggaagcg atgtacccca gcgggtgacc tacgtgcagg ccggcgccgg aagggtaggg
     4741 gaacatgtcg aggatgtagt atttggggcg cgaacctccg ttttcagtgc gatacgtttg
     4801 tttttgctcc caaatggatt tccatttctg ttcgatgtcc cgaggggaat actccataat
     4861 atactgtttt tgacttgagt aacggtagcg cacaaggcgc ctgaaaagtt tcttttttcc
     4921 ttgcaggggg cgaaattaaa aaaagttcta tttttgccgg gctttttgaa agcggccttt
     4981 cgggggcgca ttttttttga acaaaattta aattcaactt catggctacc aggaagaaaa
     5041 gttccgcaaa ggctgacgaa accttagtgg atattgtgga agtaaaagag caggcggaag
     5101 atttttttga acgctatcgc tttgtcattt tgggcgctgt gggcgcattg gtactggctg
     5161 taggcggatt ttttgcatac accaattttt acaaaatccc gaagcagcag gaagctgtgg
     5221 agcagatgta tcaggctcag attcagtttg agcgcgactc tttcacgcag gcgctcacca
     5281 atcccggcgg cggctatcag ggtttccttg atatttcgga gaattacaaa ggcaccgatg
     5341 cagccaatct ggcgctgtat tatgcgggca tctcttacct caatctgggg caatacgatg
     5401 cggctattga ctacctgaaa agctacaaac cggcaggcga tgtgatcccg gccatgaagt
     5461 tcggcgctct gggcgatgct tattctgaaa agaatgattt cggccaagcc atgagctact
     5521 acaaaaaagc tgcttctacg ggtgtcaacg aggccatcac accttactat ctcaagaaaa
     5581 tcggcatgct gcatgagcgc aactccgaat gggaccaggc cattaaggtg tatcaagaga
     5641 ttaaagacaa gtatccgacc tcgcccgacg gccgggatat tgacaaatat ctcatccgcg
     5701 cagaggccaa gaaaggctga ttccggcaat gaacgatcaa gttgtcaaat catcttcctc
     5761 cattcacatc cgggtgggat tgaatgagga aaattatccg gtacagattg aatggcattc
     5821 ggacgacgct ccggatgcca ttcagccttg taaagcgatg ctcctttctt tgtttgatga
     5881 agaaaacaag gaaacggtaa aaatagacct ctggaccaac gaaatgcagg tgatggaaat
     5941 ggatcgcttc tttttccaaa ctttgcgcgg cttggccgat acctaccaca aggccacccg
     6001 gaacgcgcgc ttagcttctg ctatgcagca gtttgtgcag tatttcggtg aagaaacgga
     6061 gattattccg cgagagaagt aggggagaga acaaacctct cttactacaa aaccggaatt
     6121 ctcacttcca ctgtcgtccc tactgcggct gcgcttatta tttctgaacc gccgcccagt
     6181 tcctccgccc tgatttttat attggccaaa ccgttgcccg aattggctcg ttgcccggca
     6241 tcgaagccgc aaccattatc ggaaatgcgc atcaggagct gattttcagt cggaacttca
     6301 aaggccaggc tcatttcggt ggcgcccgaa tacttgatgc tgttgttgat cgcttcctga
     6361 gcgatgcgaa acaaattgag ggtttgcgcg ggcgacagcg tcaaatcggc gccggcggaa
     6421 acaaggacag caagcggcgg ggcgccggca cgcaaggcaa aatcgctcac tttatcggcg
     6481 agatcgctga gagagaatgc ttcataacga atggcccaga tggtttcccg cagttgagac
     6541 atggcctgcc gggcattgtt gcgaatggtt tccagcgatt tcttttggat gtcgttatcg
     6601 gcctgcgtga tgttgcgcgc cagcgaactg ccgatgatgg tgagttcggc gccgaggttg
     6661 tcgtgcaggt cgcgggaaat gcgcagtcgt tcgcgttgca ggcgcagcgc cgattctgat
     6721 ttgatcaatt cggtttttag ttctgcttcg cggcggagtt gatgttgttt caggcgttgc
     6781 cggtgaaata cgaagcccga taccaaggcc aaaattgcca gcccggcggc ggcgagcgcc
     6841 aattgaaaac tgcgacgttg caattgtttt tgctgttggg ccagttcttt ttccttcttt
     6901 tccacctcat atttttcggc catttccgct atttgccggc tgtgttccag gttgaaaatg
     6961 ctgtctttca gggcatagct ccggttcagc caatccattg cttcctgatg caaactcagg
     7021 gccgaacagg tttccgccag catttgcatg gcgtggcgtt ggaggtcggt aaacccgatg
     7081 gcggaactca tctcaatggt tttttcaaaa tacggtttcg ccttgtccgc ttgtcccgaa
     7141 tggaggtaca tctcgccggt gttgttggtg ttgatggcca cgccggctgc atcgcccatg
     7201 cgctgccgga tgtcggttga ccgggcgagg agcgcggcgg catcatcaaa ccgtttttgc
     7261 ccgatggcca cgtcggcgag gttgctcagg gtgtaactca gggcgcggtc gttgccgttg
     7321 cgttccagca gcgcctgttc gtacaggagc acttctttcg catcgtccag ccgcccctgc
     7381 tcgatgagca ccaccgcttt gatgtctaag gcggcggtga ggcaattggt atcgcgggcg
     7441 ccggaacaaa gctgggcggc ttgttccaat tgttggaggg ctttatcgta tcgcttcagg
     7501 cgtttgaaat aattgcctaa ttctttgagg gtttcgccgc gcccggcctg gtcgccgatt
     7561 tgttcataca ggtcgagggc ctgctgatac aagggcaggc aggtttcata atcgcctttg
     7621 aggtaatacg caatgccgat gtttttgagc agagaggcct cccattgcgg ccagcggttt
     7681 ttgcgagcca gggccagcgc tttttcgccg gtttggatgg ccgtgctaaa attggaggac
     7741 gcaatctgac taaaatgctt cccgatgtat tccacctgtt cccgctcggt catgccggaa
     7801 agcgcttgct gaaggctgtc ggtttgggct ttcgcctgac tgctcaggag gaggaacgtg
     7861 aggaggaaat ggagggtggt gcatttattc atgaaatgtg attgctttta tttttctcat
     7921 gtcattttgg tcaatgagta ggacagatac agccgtcttg gttccattcc atgattgatc
     7981 tacaaggtat tcttctgtga taaagtaaat gtgaataaca ggagattgga gtctattacc
     8041 tcttccgaat aaatatcaat aagaccatgc tgtgtaattg gcgtgaaatt cagttttatc
     8101 cgttcatttt cttcaggaaa ggtgcaattc cataggctta cagcaagcaa tatgatgatg
     8161 gggattttca tagcattgat tgattttgaa gaagtaactc attccctgac gaactcatac
     8221 ctcccttccg gcaaacgaaa atacctctta ttcagcgtac tgtccggttt ggcgccgaaa
     8281 tcggagagca gccagcccgg ctccttgatt tccttttcaa attttccgaa atcgctccga
     8341 atgagcagcg tttgtgcgga atcggggtgc tgcatggccg agagcagcag cgtttcataa
     8401 ggcgccgacc aggtgatgaa caggctgtcg cgcagggcgg gcgtttcgcg cactaaatag
     8461 cgactgctgc cggtgttttg gcggccttcg gcaaggatgc tttccaagcg ctccaatcgc
     8521 tgtgtccagg gcttatggtg cagcccgatg gcggcgatgc gcaagattaa aaacagcgca
     8581 aaacccgctg tgagccagtg ttttgggcgg atggttggtg ctatatcgta ccaaaagggc
     8641 agggcagcgt acagcgtgag gggcaggcaa ttgacctcgg cgtaaaaccg gtacgggtgc
     8701 tccggcgagg caatgtgcaa cagcatcagg tggccgaggc caaacagcca catcagcaac
     8761 agcggtttcc attgtcggcg atggaggtaa aacccgctga cagccagcca caacaatggt
     8821 aaaaaatacc aatgcgccag gcaccactgc gcaaactgcc cgtgcgaggc catgctgaaa
     8881 tagtcgggaa acagggcggc gagattttct ttaaaaatgc ggaatttatc gtcgtcgtac
     8941 cagttgcctg agaataatga tttgaaaacc agcacgctgg ccatgtatcc gcccaatgcg
     9001 agatagcgcc cgttgcgcac ctcggcctga cggagcagga agaaggccca cagaaaataa
     9061 aacggtataa atatcagcgg gtgataccag gccagcaccg gcacggcgat gagcgcaacc
     9121 aatatggcgc tgatgctttt gaaacgcagg tctcgttgcg cgaaagcgaa atacaagagc
     9181 aaaaacgcca gcccctgctg ctgctctgaa gagggccagt aaaaggcgtc aaataccatg
     9241 agggtgaaca gcgtgaccaa ggacaggccc aattcctcct gcttacacac gcgtaccatg
     9301 agcgcataca cgcccaaaaa caggagcgga aacgagatgg aataaagtaa agaaaccgcc
     9361 tgaagcggca gccccatttt gatggccaac agcggcaatg cctgcaccaa cactacgccg
     9421 aagcgattga ccatgatctg gaaatcgccc tcggtgctca tcaacaccgt ctgaaatgcc
     9481 atgtcggtaa agagcgcccg ctcgcggaaa aagacggcag ccagcgccag cagagccagc
     9541 aaggcgagcc atcccaagcg gcggagtgat agaaatgagg acatcatagg cagttagggg
     9601 cgatatttca ttccggcacc tggtagtgca aaatggtttg ctcgatgatg tcgcagcact
     9661 cgtgcatttg atcttcggtc atgaccagcg gcggcgcaaa tcggatgata ttgccatgtg
     9721 tgggtttggc cagcaggccg ttttcagcca tttgcataca aatatcccag gccgtatggc
     9781 cgtcttcatc gtcggcgatg atgatggcat tgaggaggcc cttaccacgc acgcctgtga
     9841 ccaattcggt gcgacgcatg aggttgtcgc tgaggcgttg gcggaaaatt tgtccgaggt
     9901 attcggcatt ttcggccaat tgttcatctt tcactacttc taacgaggcc atagctaccg
     9961 cgcaggcgag gggattgccg ccaaacgtgc tgccgtgttc gccgggcttg atggtaagca
    10021 tgatttcgtt gcgcgccaat gctgccgaaa ccgggaaaac gccgccgccc aaggctttgc
    10081 ccaataccag aatatcgggg cggaaaccat cgtgatcgca ggccagcagc ttgccggtgc
    10141 gcccgatgcc cgtttgcact tcatcggcga tgaggaggac gttgttgttg cggcatagtt
    10201 cgtacgcttt gcgcatgtaa ccctcgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
    10321 tcgttgtagg gaatgatgac gtagccgggc atgtacgggc cgaagtcttt ggtgcttttg
    10381 gggtcggtgc tggaggaaat ggcagccagt gtgcggcccc agaagttatt ggctgcaaag
    10441 atgattttgg cctgattgac gggtacgcct ttcacgaggt aggcccactt gcgggccaat
    10501 ttcaccgccg tttcgccgcc ttccacgccg gtattggcgg gtagtacgcg gtcgtagccg
    10561 aaatattcgg tgatgtattg ttcaaaacgg cccagcatat cgttgtaaaa tgccctcgaa
    10621 gtgagggtga gtttttgagc ctgttcggtg agggccgaga tgatgcgagg gtggcaatgc
    10681 ccctgattga cagcggaata ggcagaaagg aagtcgtagt attgcttgcc ttcggtatcc
    10741 cagacgtgaa cgccttgtcc gcgcgaaaga actaccggca gggggtggta attgtgtgcg
    10801 ccgtagcgat cttccagcgc catcaaatcc tgcgaactga atatggtgtc ttgcagcatg
    10861 tcgtttggtt ttattttcgg tgtcaaaggt cggaagaaat gttggaaagg ggaaggggtt
    10921 tatcggttct gatttcggat ttccgatttc ggatttccga ttcctgattt tttgatttct
    10981 gattggcaaa ccgcctaccg aaaaccagtt tcgagtttca ggtttcggga gtagtgaagg
    11041 ggtgactcag caagtttcag cggggtagtc accccttcac taagccggag aagcaaaaac
    11101 aaggctcggt tttcgacgct gaaagacgca ggttttatgg ttgttatgtt cctctccgag
    11161 cagcggaaat cttataaatc ataaagatc
//
LOCUS       DAORLF010000107        11061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3171_length_11061_cov_1.841359, whole genome shotgun sequence.
ACCESSION   DAORLF010000107 DAORLF010000000
VERSION     DAORLF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3171_length_11061_cov_1.841359"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>371
                     /locus_tag="PK198_10340"
     CDS             <1..>371
                     /locus_tag="PK198_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39178.1"
                     /translation="KIEEEDTPEKKEEVREEFLDQVRCLLSAHRNLGEDFESITILCP
                     QNVGLCANVEILPGTDAEKTYFEILRRIIDFISPTARFHSLQSLLEKGRPIEEIFAGR
                     PYSLKTILEKTIGEDGPPDFC"
     assembly_gap    372..407
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            444..>792
                     /locus_tag="PK198_10345"
     CDS             444..>792
                     /locus_tag="PK198_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39179.1"
                     /translation="MEGIERTKEIHLSDLYSVIHSVPGVRSVKNLCLNTDVTHVPLPH
                     LDCGDEKPWPQAEWRFRLLQNAAPIFLFDNTSLTNISLSTATGAIPVNKRKAHAELTR
                     YQKSYLSDSFLDML"
     assembly_gap    793..806
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <807..>1185
                     /locus_tag="PK198_10350"
     CDS             <807..>1185
                     /locus_tag="PK198_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39180.1"
                     /translation="GAFREDLPDYISIQNEFPRVYGIGETGLPESASLLRRVQARQFQ
                     GYLLFFDQLLADYLQQLSRLRDLFSLKQESQRPEDARHTLFSAVLNQDNTPGFQKLNR
                     FFAAKDQGIVEGSVLALPVRDDQA"
     assembly_gap    1186..1196
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1197..>2480
                     /locus_tag="PK198_10355"
     CDS             <1197..>2480
                     /locus_tag="PK198_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39181.1"
                     /translation="THALSELEATLNGHLVIKAPGACLEYEKGNCDEEVCSSTYGYVI
                     RRDSDLNLVRRSLEEKNYTIDLRRDRAGWFFILRFPSISGLVLVGVGRYERAQEAENA
                     AQLAAFLGMWENNLSTSGYDPAAGGDRRHTFSLVSREMDYVHILQEMLENQSAYIHRR
                     EGFLDHLLARFSEKFTDYAVMLAGMVQDEQMRRRTSVEDKSRFLSKYDDISRNRGRAF
                     DYLQPAWGTDNISGYERRAMLLSGFTDDRRQSLCNIEVFEKGSSYRVVLDDWHNSPLL
                     ASLDTITSDFAAESVAAALHETLLSDRPFQVGYDPLTQKYTLSIRAGYVSFAYPNPID
                     SEFEALEKAKQIKALFAVQSVSDNVQVSRYAYVQELHDAQGSLVHASERQFATNEDAQ
                     AQKQLKAFISDIPGDANGRSRKLSPMPVSAGRDLD"
     assembly_gap    2481..2490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2625..>2873
                     /locus_tag="PK198_10360"
     CDS             2625..>2873
                     /locus_tag="PK198_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39182.1"
                     /translation="MPEGGAVYAAGNGRTWEIAAEDGTVLQSERIFSDIARANTAWRQ
                     AKEWGQKTAYYIPVFSSDGKAFHIELRSDTDAVLASTQP"
     assembly_gap    2874..2885
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2886..>3185
                     /locus_tag="PK198_10365"
     CDS             <2886..>3185
                     /locus_tag="PK198_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39183.1"
                     /translation="NSKPEILIEAFAAIFGNKKAKPRTPAKPDCWGFRLNNDKGEPLL
                     LSYALYPSASEALRTGMETHKDATETGRYVKSGDETNLHYIYLLKDAHNQFIAESP"
     assembly_gap    3186..3299
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3300..>5431
                     /locus_tag="PK198_10370"
     CDS             <3300..>5431
                     /locus_tag="PK198_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39184.1"
                     /translation="PSWAFALADEKERLILQSVEEYESETATGKAFEDFLCDLADMSQ
                     VKAKGNLIQTETASARVHSGDATRAAKSLSDAVTAQVFAFDVRKTPAGYRFYYYWDGP
                     DGSYEPLFESMHEFTRHEEAEQAFGAFAKNLHLATLEKTGKGKTTALTLKMGEEPVAK
                     ATAAAGGDPARVESFLDYRVTHPAGLMESYSKWVQPPQGRPEYAYRLVKKGHPFAVWA
                     CNPHLNPPDDCYPDCKEDTLEGGCYEPKPVLQYDGYRPKRACWPDKAAAANEVSKICL
                     KKPHDFTWLDICFGAADVIQKRTGENCICKYHFIIKGAIGDGPELVLFQSITGYDTEA
                     LAVAAYQEKFLEILQAASCEDNYGQDRLICTQGCFELQPDSCGKIGNYIAAVADNYDN
                     ATPAQIEQLVKWAKSFPVRFFEVWENGIKRKKYYFRLFNIPSGSFEWQSWECYDSFNE
                     AWQGFKKLMQLLACGDHCRAGQMEPSCDHAGEGVKYQIEIREVLATGQWCYETEDEAW
                     GRDLCDQIAPFCPCEEPEQEDHDDGYDSIARSEQPGSALFAAAFINLEIPPKDVPHLP
                     DLPITDHCCKLAVHPCRHTEEELSHLPTWNNTSQTPQEKFLNAITAVLLGVLQSAYAM
                     ENGLYAPIVNYQPTQNLEFLRCDYFLKGELLYNTREEALSAAAQIIARIKSGNYHIKI
                     NFDPERFTYGFELYDACCHYGAERFLAAA"
     assembly_gap    5432..5527
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5528..>6274
                     /locus_tag="PK198_10375"
     CDS             <5528..>6274
                     /locus_tag="PK198_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39185.1"
                     /translation="RVAVHPCAYDSEAKAQAALDQLMAELNDLIATSEAKRFIINKTE
                     GRHFLKITRQRTRPDTIIPAVHTDKAAPPIAESPITILDPCDEDAAPPFECDTLLAGV
                     KGFKTESGALSHAHTLLRIHSMTGTPESRYQYIIVPETECGPWTIQLVDTCCVLAKSP
                     CLFNDKYTRDSSMAAVKACTAVEGMHLLEHILLRPCVEDCKDSYVPNQPCTDSDDNLL
                     KPCNPCLLQGCPDMQCTLCWVDNPFEKDPC"
     assembly_gap    6275..6297
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6298..7462
                     /locus_tag="PK198_10380"
     CDS             <6298..7462
                     /locus_tag="PK198_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39186.1"
                     /translation="PPLSYLPFADLYSFWATLVLPSWMKRFHDADARSFFQHVLYKEA
                     PAHIALNIVWLSPRQMCRFEDTYRLWLTWMRCPDNDMDCSELPDCCGDTVRLAQYEGQ
                     NPSRQKCNLRCCMVKCLTALIPYHWCEPGIGDKEPCDCAEKIKSKVQDDRIVKQSSSN
                     YLQAENGHFLHLFRCHPAPAKAQDKQPEITHRSILSSQLETITAIPLTEAPKSTTEAR
                     SIAVESSAPALEKKKEEPPAPANGAAAERRSFNSRIQERKAEVMHLESKISDPEIFHR
                     IADSFLNSEARFSEFKKHATYLLDQTDDLSSAELPETILLKNAVWHLLDKAVEQDKEG
                     APFAHEKEFKALLAQMKVKGFKLRELAQNWDQAGKVSGVFGKSAVNRYLKWLK"
     gene            7480..>8507
                     /locus_tag="PK198_10385"
     CDS             7480..>8507
                     /locus_tag="PK198_10385"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HRF39187.1"
                     /translation="MQHIIARQVFELSLRNSGEAFAVQQAVSRAFRDHAVPAMERLFD
                     QYSTEEEWITIDRLEIHLGRISEAEWASSAWLDRMIESIKDALKQMLKNGAAGVEKKP
                     LRVARFEQWLYFLRHGRLPRHVVPAPEEEWRRLILAALGTEMRILEQLARLLRSNPEA
                     LRRLVMQHPPQFLEHLVELFTGRKATGLFAVLSETGKAAQAIAEMLTTGKPRRKLPPG
                     VGAAFTELMEALIAYEETSADPGMHDKGAADLWPSRSTLDAVRSARLLEIHCRQAVFR
                     TLSQSRSPYTADDLLQLVFRSGSLRDMLPLLVMAANGKRNPYPELKAFFKRAGFLLPA
                     ASARKPLDV"
     assembly_gap    8508..8667
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8668..>8915
                     /locus_tag="PK198_10390"
     CDS             <8668..>8915
                     /locus_tag="PK198_10390"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HRF39188.1"
                     /translation="KLPEVSSGPATDPHSSRGTAGPADTAVESEALHTPVAGLVLLHP
                     FLAMFFETVGLLEKRRFKDEWRRQKAAALLYYLATGETG"
     assembly_gap    8916..9032
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9033..9248
                     /locus_tag="PK198_10395"
     CDS             <9033..9248
                     /locus_tag="PK198_10395"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HRF39189.1"
                     /translation="AIDNWGALGSTSPDGLRGNFLVRDGKLTRTDLGRLLQVEAQSYD
                     LLLDRLPWGINMVKLPWMEDMLFVEWR"
     assembly_gap    9292..9301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9302..9935
                     /locus_tag="PK198_10400"
     CDS             <9302..9935
                     /locus_tag="PK198_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39190.1"
                     /translation="QNNDCPPIREALRQQAKNSVEETILFRLFEQIRIPETNILMAGM
                     MLTAKDMLEYLVAKSYDDNQAIRTFRSAWIAAVDSDRFYWEAFFPGVFVSLDDANMPS
                     GMTQRVAGGETEEIGGYFEAPDLDREWSRYIGWSFFKAYSVALKDSVRAAFSLPGWQQ
                     ATDSDSKILFMARHLLESVGGKRLYWALLMLIVSILLVVTHFSTLPESEP"
     gene            10035..10874
                     /locus_tag="PK198_10405"
     CDS             10035..10874
                     /locus_tag="PK198_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRF39191.1"
                     /translation="MPERETFFTAGKNAPGNASRPLIQTKLTIGQPGDKYEQEADQVA
                     DQVVQRLAQPEAITPNPTAEKPIAPKLQLKAAPAPAASQSSATPSVQTKCAECEKEEK
                     EQLEGAEALPAVQKKPIFESEANNGALQMKCAACEAEEETLQPKSDGVEAPAASSSLE
                     SRLQSSKGGGSPLPENTRSQMEGAIGADFSGVRVHTNSSAVQMNKELGAQAFTHGSDV
                     YFNAGKYDTGSTEGQRLLGHELTHVVQQGGESRQKQISNEEKKEIPQLKTDKEYIQQY
                     SWS"
BASE COUNT         2643 a         2860 c         2746 g         2209 t
ORIGIN      
        1 aaaaatagag gaagaagata ctccagaaaa aaaggaagaa gtaagggagg aatttctcga
       61 ccaggttcgc tgcctgctgt cggcccatcg aaacctcggc gaagatttcg aaagcatcac
      121 catactgtgt cctcaaaacg tagggctttg tgccaacgtg gagatactcc ccggtactga
      181 cgccgaaaaa acctactttg aaattctccg caggattatt gacttcatct cgccgacggc
      241 tcgctttcac tccctgcaaa gcctgctgga gaagggccgg ccaatagagg aaatttttgc
      301 agggaggccc tactccctta agactattct ggaaaagacc attggagagg atgggccgcc
      361 tgatttttgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag ttcaggagtt
      421 tcgggttcct cgatacagaa gaattggaag gtattgagcg aaccaaagaa attcacctgt
      481 cagacctata ctctgtcatt catagcgtgc caggggtacg ttctgtaaaa aacctctgcc
      541 tgaacacgga tgtcactcat gtacctttgc cgcatctgga ttgcggagac gaaaaacctt
      601 ggcctcaggc cgaatggcgt ttcaggttgc ttcaaaatgc agcgccgata ttcctctttg
      661 acaatacttc cctgaccaat atttccctat ctactgctac cggcgccatc ccggtcaaca
      721 aaagaaaggc acacgcagag ctgacccggt accagaagag ctacctttcc gattcgtttc
      781 tggatatgct tgnnnnnnnn nnnnnnaggg cgccttcagg gaggatttgc cggattacat
      841 ctccatccag aacgaatttc ccagggtcta tggcataggc gaaacgggcc tgcccgaatc
      901 tgcatcgctg ctacggcgcg ttcaggctcg ccagttccag ggctatttgt tgtttttcga
      961 tcaactgctg gccgattatc tccaacagct aagccggttg agggatttgt tttcgctcaa
     1021 gcaggaatcc caaagaccgg aggatgcccg gcacaccctg ttctcggcag ttttgaacca
     1081 ggacaacacc cccggttttc agaaactcaa ccgcttcttt gcagcaaaag accaaggtat
     1141 tgtggaaggc tctgtgctgg cactgccggt gcgagacgat caggcnnnnn nnnnnngacc
     1201 cacgccctca gcgaactgga ggcaacattg aacggccatc tcgtcattaa agcccccggc
     1261 gcctgcttgg aatacgaaaa aggcaactgt gacgaggagg tttgttcttc cacttatggg
     1321 tatgtcattc gacgggattc agacctgaac ctcgttcgcc gctctttgga agaaaaaaac
     1381 tacaccattg acctccggag agaccgcgca ggctggtttt ttatacttcg ctttccatcc
     1441 atcagcggct tggtactggt cggcgtcggc cgttacgaac gggcgcagga ggctgaaaac
     1501 gccgctcaat tagccgcttt cctcggtatg tgggagaaca acctttccac atccggatac
     1561 gaccctgccg caggaggtga tcgccggcac accttctcct tggtatcccg tgagatggat
     1621 tatgtacaca tcttgcagga aatgctggaa aaccaaagcg catacataca caggcgagag
     1681 ggttttctgg atcatttgct ggccaggttt tcagaaaaat ttaccgatta tgcagtcatg
     1741 ttggccggca tggtgcagga cgagcaaatg cgtcggcgca caagcgtgga agacaagtcg
     1801 cggtttttat ccaagtatga cgacatcagt cggaacagag ggcgggcttt cgactacctg
     1861 caaccagcat ggggaaccga caatatctcc ggctatgagc ggcgggctat gctcctttcc
     1921 ggctttaccg acgaccggcg ccagtctttg tgcaatattg aggtttttga aaaaggctct
     1981 tcttacaggg tggtactcga cgattggcac aacagcccgc ttttagcatc gcttgacact
     2041 attacttccg atttcgctgc cgagagtgtg gccgcagcgc tccacgagac gcttttgtcc
     2101 gatcgccctt ttcaggttgg atacgacccc ctcacacaaa aatacacgct ttccattaga
     2161 gccgggtatg tctcgtttgc gtatcccaat cccattgatt ccgagtttga ggctttggaa
     2221 aaagccaagc agatcaaagc attatttgcc gttcagtctg tatccgacaa tgtgcaggta
     2281 agccggtatg cttatgtaca ggaactgcac gatgctcagg gcagccttgt tcatgcctct
     2341 gaacgccaat ttgcaaccaa cgaggatgca caggcgcaaa aacaattgaa ggcatttatc
     2401 tccgacatac ccggcgatgc gaatggccga agccggaaac tgtcgcccat gcccgtatct
     2461 gccggcaggg atctcgatat nnnnnnnnnn ccttcaaccg ccaaattttg ccatgcccga
     2521 cccgttggaa gtggcatcgc ttcgagcaga atacccgctc ctacctgagt tcggaggcag
     2581 cctttttgtc ggagagcgag gcatgggctg atattgcgaa aaatatgccg gagggcggcg
     2641 ccgtgtacgc tgccggcaat ggccgcacct gggaaatagc cgcagaagac ggtacagtct
     2701 tacaaagcga aaggatattt tccgacatcg caagggctaa taccgcatgg aggcaggcca
     2761 aagaatgggg gcaaaaaaca gcatattaca tcccagtctt ttcttccgac ggcaaagcgt
     2821 ttcacatcga attgcgcagc gacacggatg cagtcttggc ctccactcag cccnnnnnnn
     2881 nnnnnaatag taagccggag atacttattg aagccttcgc agctattttt ggcaataaaa
     2941 aagccaagcc cagaacgcct gcaaaacccg actgctgggg gttcagactg aataacgata
     3001 aaggcgagcc gctgctcttg agttatgcgc tatacccctc tgcatcagaa gcgctgcgca
     3061 ccggtatgga aacgcataag gatgcgaccg aaaccggccg ctatgtgaaa tccggcgatg
     3121 agaccaacct ccattacatc tacctactca aagacgccca taatcaattc attgcagagt
     3181 cgcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3301 cctcttgggc ttttgccctg gccgacgaaa aggaaaggct catcctgcaa tcggtagaag
     3361 agtacgaaag cgaaactgcc acaggtaagg catttgaaga ttttttgtgc gatctggcag
     3421 atatgagcca agtgaaagcc aaaggcaacc tgatacaaac cgaaacggca agtgcgcggg
     3481 ttcattccgg ggatgccacc cgcgcagcca agagcctttc agatgccgtt actgcccaag
     3541 tgtttgcttt tgatgtgcgg aaaactcctg ccggttaccg tttttactac tattgggacg
     3601 ggccggacgg cagctatgag ccgctttttg aaagtatgca cgagtttaca cgccatgaag
     3661 aggcggagca agccttcggc gcatttgcca aaaacctgca cctcgccact ttggagaaaa
     3721 ccggcaaggg caaaaccacc gccctcaccc tcaaaatggg ggaagagccg gtagccaagg
     3781 caacggccgc cgccggcggc gatcctgcac gagttgaatc cttcctcgat tatcgcgtta
     3841 cgcatcccgc cggcctgatg gagtcctatt ccaaatgggt gcagccaccc cagggtaggc
     3901 cggaatacgc ttaccgcttg gtgaaaaaag gccacccctt tgctgtgtgg gcttgcaatc
     3961 cccacttgaa tccgcctgat gattgctatc cggattgtaa agaagatacc cttgaaggcg
     4021 gctgctacga accgaagcca gttttgcaat acgacggcta caggccgaaa agagcttgct
     4081 ggccggataa ggcggcggcg gcaaatgaag tgtccaaaat ttgtctgaaa aaaccgcatg
     4141 atttcacctg gctcgatatt tgtttcggcg ctgcggatgt gatacagaaa cgcacaggcg
     4201 agaactgtat ctgcaaatac cacttcatca taaaaggcgc catcggcgac gggccggaat
     4261 tggtactgtt tcaaagtatt acaggatacg ataccgaggc tttggcggta gctgcctatc
     4321 aggaaaaatt cctcgaaatt ttgcaggccg cttcctgcga ggacaactat ggccaagacc
     4381 gcctgatatg tacgcagggc tgctttgagc tacaaccgga ttcttgcggg aagatcggca
     4441 attatattgc cgctgtcgcc gacaactacg acaacgcaac ccccgctcag atcgaacagt
     4501 tggtcaaatg ggctaagagc tttcctgtcc ggttctttga agtgtgggaa aacggcatca
     4561 aacgaaaaaa atactacttc cgcttattca acataccctc cggaagcttc gagtggcaaa
     4621 gctgggaatg ctacgatagc ttcaacgaag cttggcaggg attcaaaaag ctgatgcagt
     4681 tgctggcttg tggagatcac tgccgcgccg ggcaaatgga gccgagctgt gaccatgccg
     4741 gggaaggggt gaaataccag atagagatac gcgaggtatt ggccaccggc caatggtgtt
     4801 acgaaacaga agatgaagct tggggccgcg acctctgcga tcaaatagcg cctttttgcc
     4861 cttgcgaaga accggagcaa gaagaccacg atgatggtta tgactccata gcccgatccg
     4921 aacaacccgg cagtgcgctt ttcgctgcgg catttatcaa tctggagatt cctcccaaag
     4981 atgtccccca ccttcccgac ttgcccataa cggatcactg ctgcaaactg gcggtacatc
     5041 cttgccggca cactgaggag gagctatcgc acttgcccac gtggaataat acatcgcaaa
     5101 caccacagga gaaattccta aacgcgatta cggcggtact acttggggtg ctgcaaagtg
     5161 cctatgccat ggaaaatggg ttgtatgctc caatagtaaa ttaccagccg acgcaaaatt
     5221 tggaatttct tcgttgcgac tattttctga aaggcgaatt gctctataat acaagggagg
     5281 aggcgctctc tgccgctgcg caaatcatcg cccggataaa aagcggaaat tatcacatca
     5341 agataaactt tgatccggaa cgcttcactt acggctttga attgtacgat gcctgttgtc
     5401 attacggcgc cgaacgattc ctggcggctg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnacc gcgtagcagt tcatccttgc gcttatgact cggaggccaa agcccaagcc
     5581 gccttggacc aactgatggc tgaattgaat gatctgattg caacgtcgga agccaagcgt
     5641 ttcattatta acaaaacgga aggcaggcac ttcctgaaaa taacccgaca acgaacccgc
     5701 cctgatacca ttatcccggc tgttcatacg gataaagcag cgccccccat tgcggagtcc
     5761 cccattacca tcctcgatcc atgcgatgaa gatgcggctc ccccctttga atgtgatacg
     5821 ctcctggccg gtgtaaaagg attcaagacc gaatccggcg ctttgagcca cgcccatacg
     5881 ctcttgcgca tacacagcat gaccggcaca ccggaaagcc ggtaccagta tatcatcgtg
     5941 ccggagacgg agtgcggccc ttggaccatt caattggtgg acacctgctg cgtactggcc
     6001 aagtctccct gcctcttcaa cgacaaatac acgcgcgaca gcagtatggc ggcggtgaag
     6061 gcgtgtacgg cggtggaagg catgcacctg ctggaacaca tcctgctgag gccctgcgtt
     6121 gaagattgca aagacagcta tgtgcccaat cagccatgca ccgactccga tgataacttg
     6181 ttgaaaccct gcaacccctg cctgctgcaa ggatgccctg acatgcagtg tacattgtgc
     6241 tgggtggata atcctttcga aaaagacccc tgcgnnnnnn nnnnnnnnnn nnnnnnnccc
     6301 gccgctcagc tacctcccgt ttgccgattt gtactccttc tgggctaccc tggtgttgcc
     6361 gtcctggatg aagcgcttcc acgacgccga tgcgcgctcc tttttccagc atgtgctgta
     6421 taaggaagcg ccggcgcata tagccctcaa tatagtttgg ctcagccccc ggcaaatgtg
     6481 tcgtttcgag gatacctacc gactttggtt gacctggatg cgctgccccg acaacgacat
     6541 ggactgctcc gaactgcctg attgctgcgg agacaccgtc cgcttggctc aatatgaagg
     6601 tcagaaccca tccagacaaa aatgcaatct ccgctgctgc atggtaaaat gcctgactgc
     6661 cctgatacct taccactggt gcgaacccgg catcggcgac aaggagccat gtgattgcgc
     6721 agaaaaaatc aaaagcaaag ttcaggatga cagaatcgtg aagcagagca gttccaacta
     6781 cctgcaagcg gaaaacggac acttcctcca cctcttccgt tgccatcctg ctccggcgaa
     6841 ggcccaggat aagcagccgg aaatcacaca ccgctccata ctttccagcc agttagagac
     6901 gatcactgct atccctttaa ccgaggcgcc taaatccact actgaggcgc gcagcattgc
     6961 agtagagtct tcggctccgg cgctggaaaa gaaaaaagaa gaacctcctg cacctgccaa
     7021 tggcgcagcc gccgagcggc gcagtttcaa cagccggata caggagcgca aagcagaggt
     7081 gatgcacttg gaaagtaaga tatccgaccc cgaaatcttc caccgcatag ccgacagctt
     7141 cctgaacagc gaggcgcgtt tctccgaatt taaaaaacac gccacttacc tgctcgacca
     7201 aacggatgac ctttcctccg ccgaactgcc ggaaaccatc ctgctgaaaa acgcagtatg
     7261 gcacctcctg gacaaagccg tagaacagga caaagaaggc gcccccttcg ctcacgaaaa
     7321 agaattcaaa gccttgcttg cgcaaatgaa ggtcaaaggt ttcaagctca gggaactggc
     7381 ccaaaactgg gaccaggccg gcaaggtaag cggggttttc ggcaaaagcg ccgtcaaccg
     7441 gtatttaaag tggctgaaat gaccctgaaa taactcctca tgcagcacat cattgcacgg
     7501 caggtattcg aactcagctt gcgcaacagc ggggaggctt tcgccgtgca acaagccgtg
     7561 agccgcgctt ttcgcgatca tgccgttccc gctatggagc gcctgttcga tcaatacagc
     7621 acagaggaag agtggatcac catagaccgc ctggaaatcc acctggggcg gataagcgag
     7681 gctgaatggg cttcttcggc ctggttggac cgcatgatcg aaagcatcaa ggatgcgctg
     7741 aagcagatgc tgaagaatgg cgctgcaggt gtggagaaaa aaccgcttcg ggtagcccgg
     7801 tttgaacagt ggctctattt tttgcgccac ggaaggcttc cccggcacgt cgtccccgct
     7861 cctgaagagg aatggcgccg gctcatcctc gccgcattgg gtaccgaaat gcggattttg
     7921 gaacaattgg cccgtttgct gcgcagcaat cccgaagcgc tccgccgatt ggtcatgcaa
     7981 cacccaccgc agtttttgga gcacttggta gaactattca ccggtcgcaa agcaaccgga
     8041 ctttttgctg tgctctcaga aaccggcaaa gctgcacagg caattgccga aatgctgacg
     8101 accggcaagc cgcgcagaaa actgccgccc ggcgtcggcg ccgcattcac tgagttgatg
     8161 gaagcgctga ttgcttatga ggaaaccagt gctgatccgg gaatgcacga caagggcgct
     8221 gccgatttgt ggccttcccg gtccaccctg gatgcggtgc gatctgcccg gctgttagag
     8281 atacactgcc ggcaagcggt cttccgaacc ctgtcgcaat cgcgatctcc ttacactgcc
     8341 gacgatctgc ttcaattggt tttccgttcc gggtcgctcc gggatatgct cccccttttg
     8401 gtcatggcgg ccaatggaaa gaggaatccc tatcctgaac tcaaggcgtt tttcaaacgg
     8461 gcaggcttcc tgctgcccgc cgcatccgcc cgcaaaccat tggatgtnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa ttgccggagg tctcctccgg cccggcaacc
     8701 gatccacatt caagccgggg cactgccggc ccggcggata ctgctgtcga atcagaagcc
     8761 ctgcacactc ccgtggccgg tttggtattg ctccacccat tcctcgccat gttttttgag
     8821 accgtcggcc tcttggaaaa acgccggttc aaggatgaat ggcgccgcca aaaagcagcg
     8881 gctttactct actacttggc caccggcgag accggnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccattga caactggggc gcgctgggca
     9061 gcacttcgcc cgacggcctg cggggcaatt tcttagtgcg cgacggcaaa ctgacccgca
     9121 ccgatttggg ccggctgctc caggtcgaag cccaatccta cgacctcctg ctcgaccgcc
     9181 tgccctgggg catcaacatg gtgaaactgc cctggatgga ggacatgctg ttcgtggagt
     9241 ggaggtagtc tgtcccaaag aataaccatc gaaaacctaa gcattatgaa gnnnnnnnnn
     9301 nccaaaataa tgattgtccg cccatcaggg aagcgctgag gcaacaagca aaaaattccg
     9361 tagaggaaac aatcctgttt cggctgttcg aacaaatcag gatacccgaa acgaacatcc
     9421 tgatggccgg catgatgttg acggccaaag acatgctgga atatctggta gccaaaagct
     9481 atgacgacaa tcaggccatc cgcacatttc gctcagcatg gattgccgcc gtggattccg
     9541 accgtttcta ttgggaggcg tttttccccg gagtatttgt ctccttggat gacgccaata
     9601 tgccgagcgg aatgacacag cgggttgccg ggggagaaac cgaggaaata ggcggctact
     9661 ttgaagcgcc tgatttggac cgcgaatgga gccgttacat cggctggagt tttttcaaag
     9721 catactctgt tgcattgaag gattctgtaa gagcggcctt tagcctgccg ggctggcaac
     9781 aagccaccga ttcggattcc aaaatcctgt tcatggccag gcatctgctt gaaagcgtgg
     9841 gaggcaaacg cctgtattgg gcactgctca tgcttattgt ttctattctt ctggtcgtaa
     9901 cccatttttc caccctgccg gaaagcgaac cctaaacctt gaaaaccgta caaaaacaga
     9961 gcacggctac caccgccgcg caacccaaga cggggcgccc tttcttccag aagtcgggag
    10021 aagaagcgat ggccatgccc gaacgggaga cttttttcac cgcaggcaaa aatgcacccg
    10081 gcaacgcctc acgcccactt attcaaacca aactcaccat cggccaaccg ggagataagt
    10141 acgaacagga ggcggatcaa gtggcggatc aggtggtgca gcggctggca cagccagagg
    10201 caatcacccc aaatccaacg gcagaaaagc cgattgcacc caaactacaa ttaaaagcag
    10261 cccctgcccc ggcagcgagc caatcctccg caacccccag tgtgcaaacc aagtgcgccg
    10321 aatgtgagaa agaggaaaaa gagcaattgg aaggagctga ggctttgccg gccgtgcaga
    10381 agaagccgat attcgagagc gaggcgaata acggcgccct acaaatgaaa tgtgccgcct
    10441 gcgaagccga agaagaaacc ctgcaaccca agtcggacgg cgtcgaagcc cctgccgcct
    10501 cgtccagctt ggaatcccgc ctgcaatcct ccaaaggcgg cggctcgccc ctgccggaaa
    10561 acacccgctc gcagatggaa ggcgccatag gggcggattt ctccggggta cgggtgcata
    10621 cgaatagctc tgcggtgcag atgaataagg agctgggcgc gcaagccttc acccatggca
    10681 gcgatgtgta ttttaatgcg gggaaatatg atacggggag tacggagggg cagcggttgc
    10741 tggggcatga gttgacgcat gtggtgcagc aggggggaga gagccggcaa aaacaaattt
    10801 caaatgaaga aaagaaagag attccacagt taaaaactga taaagaatat attcaacaat
    10861 attcttggag ttaatttgtt gatgatgtta cttctcccgt tgaatctaca tgggatagag
    10921 ctacagaatt gggtagaagt gcatacgata ctgcgattga cgtggcagat actgcaattg
    10981 atacagcggt tggaatcggc gaaagagcga tctataccgc ccgtaatatt ggaggttcca
    11041 tttatgacac tgcgataagt g
//
LOCUS       DAORLF010000108        11023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3187_length_11023_cov_1.938457, whole genome shotgun sequence.
ACCESSION   DAORLF010000108 DAORLF010000000
VERSION     DAORLF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3187_length_11023_cov_1.938457"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>277)
                     /locus_tag="PK198_10410"
     CDS             complement(<1..>277)
                     /locus_tag="PK198_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39192.1"
                     /translation="VDSYFTQRPGHCELRAVRDSVVLSMSKKNLDVLLMSHPILRQFY
                     DNLVIQALVEENELKLKIISHNAEQLYQYLLDELPAVVQRVPSKYIAE"
     assembly_gap    278..375
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    644..844
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <845..2126
                     /locus_tag="PK198_10415"
     CDS             <845..2126
                     /locus_tag="PK198_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39193.1"
                     /translation="QNATTKKQLKNFAVHLRSAFGEISPKETPEDWLDAFQFLTECLD
                     KTLDPAVKSVVFLDELPWLAGRKSGFLDGLSYFWNSWAVKKNVVVVVCGSAASWMIRH
                     VVYNKGGLHNRITRRIHLMPFNLYETDAYLRSRNVALNPFQVVQLYMAMGGIPHYLKE
                     VDGSKSAIQNIEQICFSGTGLLHDEFLKLYPSLFDDATHHIEIIKALGGKWKGLTRQE
                     IIDATGLPDGGNFSRYLEELVHSGFISAFNTFGKKQKDALYRLTDEYSLFYLHFIQPL
                     HQNTYERDTWQNMSQTSHFKSWSGYAFENICLKHIEQIKKALGISGIFSTSGSYYHKG
                     NSDQKGVQIDLLIDRKDSVINLFEIKFSAEPYTLTQSYAHDLLQKIASFKALTGTRKQ
                     VFLNMLTTQGLQPNTHSIGVVHAAFSLDVLFEKA"
     gene            2189..3127
                     /locus_tag="PK198_10420"
     CDS             2189..3127
                     /locus_tag="PK198_10420"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRF39194.1"
                     /translation="MDSTARHWFFIVNPAAGHGWAGRHWPEIAAQLDQHLPGYQFAFT
                     THRGHAADLAENALQNGFRCIAAVGGDGTNNEAVNGLMRQNLVPLSDILYTLLPVGTG
                     NDWIRTHGIPRKLRLWLQMASGGTVIEQEVGIVDFMQDEGREQRRYFVNVAGLAYDAF
                     VVQYSEQNKSRVGGSLMYLALILFCLFKYRLSNMRVRFDGQERSGLYYTINAGVCRYS
                     GGGMQVVPHADPQDGLLALTLAGKLSKGGVLLNTWRFYNASIGRHPKVELHQVRHISV
                     ESGDGTPVLVEADGEFLGQGPVRISLDERRLRVLRP"
     gene            3156..>3910
                     /locus_tag="PK198_10425"
     CDS             3156..>3910
                     /locus_tag="PK198_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39195.1"
                     /translation="MKPTNVIFSFCAAVCLLLSCNRQSPPDFPASVLDARYASGRLEG
                     VFAHADSLKYARQYTGATHAFLALAADAALSEEDACYLHNQLADLYLLQYKLDSSEWH
                     LSVVQDIMSKMSQGVSAAVQADYWINKGRLAFRLRHPKEALDALTRSLDLYEAAYPGK
                     THLKKALAHTFLGLCHYAFEKDNRPFYQHVETAYDIVRSDSLLWPYSVETELAKCYVS
                     FWKRNFKALEAHAENVKLLSASQPYPDTVALARA"
     assembly_gap    3911..3933
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3934..5962
                     /locus_tag="PK198_10430"
     CDS             <3934..5962
                     /locus_tag="PK198_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF39196.1"
                     /translation="RYYLAKGNEENAAAAIRMLDTAQILAAAVEGPHQLDLISSYIFY
                     QISDTAQFNALLDTIQRMEQRMPEYRAYTDRILGRTALMNGNQAAAKQSYARFLQQHG
                     RDSIVSEEIVDEAYAILSFLHLQGLDGRRPQIDSALFWLGQIFVHDTPWEGQLLRAEQ
                     LLQPDKYMLSPYPFWGLNRLGGILLAEYELHRKERSLHQALRAFVLSDSLLFAQNPAY
                     DETVILAFHREISGDLYSGALKALYALDSLHPAETKYRHLAAHFIDRSKSDVLFRFLP
                     GNLSSDSLRVLKSRIDRLSAAPQRTPLDNRQLAYDLDAIENFTFNQKQAAYRFADVPA
                     LQNSLDAETAVLEYKICADETWLLYTDQQQSRLHRIGISANELRQLTDSLALFLNNGK
                     TFHIPDYERHATALYDFLLRPFESELRRHRRWAIVPDGVLYRLPFEALITRASGGPPA
                     FVVTEFKDLAVTYSPALKIRQMNLERKKGGKPAAGASFYNYGPDNNDGLVCSEKEWLA
                     LSGRDRRARKYMGSRCSKQAFIQDWQARRRHTVHLSLHGQADAADLLGAKIWFGPGAT
                     DVMYGFEIAHTPGYAEEVILSACETADGAWATGEGVYSMARSFFQAGACSVMATIWKV
                     ENCHNAHILGSFYAAPASLSGAQALADAKRAYLRSQKAYQPYYWAGMVCME"
     assembly_gap    5998..6041
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6129..>6354)
                     /locus_tag="PK198_10435"
     CDS             complement(6129..>6354)
                     /locus_tag="PK198_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39197.1"
                     /translation="SKDAPSIPKKQYTAEKKLPGAGSVGGGTLLVQTGGTFFVISDNC
                     PTGKMEWICFSKTSRSYYSDSRLSTIRGLF"
     assembly_gap    6355..6405
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6563..7147
                     /locus_tag="PK198_10440"
     CDS             6563..7147
                     /locus_tag="PK198_10440"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF39198.1"
                     /translation="MAKKNFLFGLFSLLVLLGCNHNPVASFTFEVKEDGEVVFVNSSE
                     NSKSFQWEFGDGTFDVASNPTHKFSANGKYDVKLTATNGKGSHSSVVTLNLDNVAAAN
                     YVFWTKHSNYLPVEIYIDGALQGNIRKSFSSALDCFAPGALTVSTTPGNHRFRARGRN
                     GSEWTEIELEFVKGDCKYLSLNRTGLSKFSRNFA"
     assembly_gap    7282..7301
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7302..8043
                     /locus_tag="PK198_10445"
     CDS             <7302..8043
                     /locus_tag="PK198_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF39199.1"
                     /translation="EGNKETEDDKFNRVDILVENNKGELVIVEIQNTREADYFQRMLY
                     GTAKVIVEHIAEGQPYSQVKKVYSVNIVYFDLGQGDDYVYHGTTTFIGIHSNHELELS
                     EKQKELFKKTSVSDLYPEHYVIKTNRFNEVAKDTLDEWIYFLKTAEIKDEFTAKGLKA
                     ASQKLDILKLEPADRKEYDRYMEAQRSDASFVETVKLEITSAVKSREEEIAESALTEG
                     ASADFVVKITGLSIEEVRSIADRLGLNK"
     assembly_gap    8223..8232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8244..>9099
                     /locus_tag="PK198_10450"
     CDS             8244..>9099
                     /locus_tag="PK198_10450"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HRF39200.1"
                     /translation="MITDNPYGSTEYMMGWVSTCFFGLGIPIGLFQTFDKRPQIVITE
                     NGIWDRTTHQDEIKWEQIIEAYPIDIHGQKFISLVTDNTFVFKKKTSRRAAAINQFIG
                     AQKLNLRLGQINIDEIGLTNFINQITKETIEERRRSIKNFKIKETRFTYSDLQKIFLY
                     IFLSVALLVLTLSSYVVFWIVMGIMGFSAITARWKPDNLTIRKYAWIGTWLGLVNMVL
                     VLGAIKIYDNVTENVGEQLAIEIEEFHSLNSTYPKEIKSLTRNLEFNFLERFFADQTH
                     QMEDRVVPL"
     assembly_gap    9100..9109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9368..10999
                     /locus_tag="PK198_10455"
     CDS             9368..10999
                     /locus_tag="PK198_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRF39201.1"
                     /translation="MFSKQQRKIKRISEFVAILSKYGFKDILARMNIGAPAAAEPLVA
                     DSEYSRIRKVLEELGPTFIKLGQTLSNRDDLLPKALIEELQRLQDRVEVLPMNIHEVL
                     EDNFGPDYKDSFQHIHTEALAAASIAQIYRAVLSGGEQVVLKVKHPSVEHTIETDLLL
                     MKDVARVLTQYFEFAEKINLMQAFSAFEKSLLAELSFVNERKNIERLAQNFKGDANAY
                     VPKVYPHLSNNEVLCMEFIDGAKITDNGYHDRHGLDVRQLAQNGLDIYLKQILEHGFF
                     HADPHAGNIMTLSDGRIAFIDLGAMGEIYQSDRVILEDLILHLAAQNPAKLVEILKRM
                     ALRMDVPDERKLHNDIADVLNMVNTSSLEDLKITVLLDKFKEILFENKIIMPDYFMLL
                     VRGIILIESVGRTLNPDINIVKSMEPYIAGVLQKRFSPDYLYRQGIAKLSELGDDLQN
                     VPVEVRQILKKLHDGNLSVQVKNDETRQLNATVEKGLANLVMAVVLSALLLSLTLLLV
                     SNTRLTLSAWSVPAVLVLCCSVVLSGLLAIRLLRR"
BASE COUNT         2797 a         2538 c         2630 g         2601 t
ORIGIN      
        1 actcggcgat gtatttggag ggtacccttt gcaccacggc gggcagttca tccagcaggt
       61 attggtagag ttgctcggcg ttgtgggaga tgatttttag tttcaattca ttttcctcta
      121 ccaatgcctg tatgactaag ttgtcgtaaa actgccggag gatggggtga gacataagca
      181 gcacatccag atttttctta gacatggaca gcaccaccga atcgcgtaca gcccgcagtt
      241 cgcaatggcc gggtcgctgg gtgaaatagc tgtccacnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnngacga tgaaatgaat atgggtgcag gtcttgcccg gcatcagcaa
      421 aacggtgttg gatttcagtt gccggacctc gatgttgtcg gctatgcgct gccgaacctc
      481 ctccgaaatg tggaggtgtt ccaacagggc aaaaagaggc gacaaaactt ctgatttttc
      541 gatttgtatc atggctgttc aaatgtggtg gttaggggtg gagcaaggta tgaaattttc
      601 aaaaataaac aagataaatg gctgaaacta tctatatttg catnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnngcaaaa tgcaacgacg aagaagcagt tgaaaaactt tgctgtacat cttcgcagcg
      901 cctttggcga aatcagcccc aaagaaacgc ccgaagactg gttggatgcc tttcaattcc
      961 tcacggagtg cttagacaaa acgctcgacc ctgctgtaaa atcggtggtt tttttagacg
     1021 agttgccgtg gctggcaggc cggaaatcgg gttttttgga tgggttgagt tacttctgga
     1081 acagttgggc cgtgaaaaaa aatgtggtgg tggtcgtttg cggttcggca gcttcctgga
     1141 tgattcggca tgtagtctat aacaaaggag ggctgcacaa tcgcataacg cggcgtattc
     1201 acctgatgcc cttcaacctg tatgagaccg acgcttatct ccgcagccgc aatgtggcgc
     1261 tcaacccctt tcaagtggtg cagttgtaca tggcaatggg tggcatcccg cattacctga
     1321 aggaggtgga cggcagcaaa agcgccattc aaaatattga gcagatttgt ttttccggca
     1381 caggactgct ccatgacgaa tttctaaaac tttacccctc cttgttcgac gatgccacac
     1441 accacattga gattattaag gcattgggcg ggaaatggaa ggggctgact cgtcaggaaa
     1501 ttattgatgc tacggggctg cccgacgggg gcaatttttc acgatatttg gaagagctgg
     1561 tacactcggg ttttatatcc gcattcaata cattcggcaa aaaacaaaag gatgccctgt
     1621 accgcctcac cgatgaatac tccttgtttt acctgcactt tatacagcca ctccatcaaa
     1681 atacctatga gcgggacacc tggcaaaaca tgagtcaaac ctcgcatttc aaaagttgga
     1741 gcggctatgc ttttgagaat atttgcctta aacacattga gcaaatcaag aaagcactgg
     1801 gcatttcggg cattttttcc acgtcgggca gttattacca caaaggcaat tctgatcaaa
     1861 aaggcgtaca aattgacctt ctgattgata gaaaagacag tgttatcaat ctttttgaaa
     1921 tcaaattttc tgccgaaccc tataccctga cccaatctta tgcgcacgat ttattgcaga
     1981 aaatagcctc tttcaaagcg ttgaccggca cccggaaaca ggtttttctc aatatgctga
     2041 ctacgcaggg cttgcaaccg aacacgcata gcatcggcgt ggtccatgcg gcgttttcgc
     2101 ttgacgtttt gtttgagaag gcctgaaagg tttttttaat gcacatttcc cccgcttgcc
     2161 gtatcattgc actcctgatg caatcaatat ggacagcacc gcccgccact ggtttttcat
     2221 cgtcaatcct gctgccggtc atggctgggc gggcaggcac tggcccgaaa tagccgcgca
     2281 actcgaccaa cacctccccg gataccaatt tgcattcacc acccaccggg gccatgccgc
     2341 cgatttggcc gaaaatgccc tccaaaacgg cttccgctgc attgctgccg tgggcggcga
     2401 cggcaccaac aacgaggccg tcaacggact gatgagacaa aacctcgttc cgctctccga
     2461 tatcctctac accctgctgc cggtcggtac cggcaacgac tggattcgca cgcacggcat
     2521 tccccgcaag ctccggcttt ggctgcaaat ggcttccggc ggcaccgtca tcgagcagga
     2581 ggtgggcatc gttgatttta tgcaggacga gggccgggag cagcgccgtt atttcgtcaa
     2641 tgtggccgga ttagcctatg acgcctttgt ggtgcagtat tccgaacaaa acaaaagcag
     2701 ggtaggaggc tcgctgatgt atctggcgct catcctgttt tgcctgttca aataccggct
     2761 ctcgaacatg cgcgtccgtt tcgacgggca ggaacgcagc ggcttgtatt acaccatcaa
     2821 tgcgggagtg tgcaggtatt cgggcggcgg tatgcaggtg gttccgcacg ccgacccgca
     2881 ggacgggctg ctggccctca cgctggccgg aaagctcagc aaagggggcg ttttgctaaa
     2941 cacctggcgt ttttacaatg cttccatcgg gcggcacccc aaagtggaac tgcatcaggt
     3001 gcggcacatc tcggtggaaa gcggcgacgg cacgccggtg ctggtagagg ccgacggaga
     3061 gtttttggga cagggccccg tgcgcatctc cctcgacgag cggaggctgc gggtgctgcg
     3121 gccttgagag gacatccctt caaaaacagc atattttgaa accaacaaac gtcatttttt
     3181 ctttttgcgc agcggtatgt ctgctgctct cctgcaaccg gcagtcgccg cctgactttc
     3241 ctgcctcagt gcttgatgcc aggtatgcat ccggccgatt ggagggcgtg tttgcccatg
     3301 cagacagcct gaaatacgcc cggcagtaca ctggggccac ccatgcgttt ttggccttgg
     3361 cagcagatgc agccttgtcg gaagaagatg cgtgttacct ccacaatcaa ttggccgatt
     3421 tgtacctgtt gcaatacaaa ttggatagtt cggaatggca tttgtctgtt gtacaggaca
     3481 ttatgtccaa aatgagtcag ggcgtttccg ctgccgtaca ggccgattac tggatcaaca
     3541 aagggcggct ggcgttcaga ttgcgacatc cgaaggaagc cttagatgca cttacccgtt
     3601 ctctggattt gtacgaagcg gcctatcccg gcaaaacaca cctgaaaaaa gccctcgctc
     3661 ataccttcct cggactttgt cattacgcct ttgaaaaaga caaccgacct ttttaccaac
     3721 acgtcgaaac tgcctacgac atcgttcgct ccgacagcct gttgtggccg tacagcgtgg
     3781 aaacggagtt ggccaaatgc tatgtcagct tttggaaacg caatttcaag gcattggaag
     3841 cacatgccga aaatgtcaaa ttactgagcg cctcccagcc ataccccgac acggtggcat
     3901 tagcaagagc nnnnnnnnnn nnnnnnnnnn nnngaggtat tatttagcta aaggcaacga
     3961 ggaaaacgcc gccgccgcga tccgcatgtt ggataccgcc cagattctgg ctgcagccgt
     4021 ggaaggcccg caccagttgg acctgatctc ctcctacatc ttctaccaga tatccgacac
     4081 ggcgcagttc aatgccttgc tcgataccat tcagcgcatg gagcaacgca tgccggaata
     4141 cagggcttac accgatagaa ttttgggcag gacagccctc atgaacggaa accaggctgc
     4201 cgcaaaacaa agctatgcgc gcttcttgca gcaacacggc cgcgactcca tcgtcagcga
     4261 ggaaatagtg gatgaagcct acgccatcct gagttttttg catctgcaag gcttggacgg
     4321 gcgaaggccg cagatagatt cggccttgtt ttggctgggg cagatttttg tacacgatac
     4381 gccctgggaa gggcaactgc tgcgcgccga acaactgctg cagcccgata aatacatgct
     4441 gagtccttat cctttttggg gcctgaacag gttgggtggt atcctcctcg ccgagtacga
     4501 actgcaccgc aaggaacgct ccctgcatca ggcgctccgg gcttttgtac tgagcgacag
     4561 cctgttgttt gcacaaaacc ctgcttatga cgaaactgtc atcctcgctt ttcaccggga
     4621 aatcagcggc gacctgtaca gcggcgcact caaagccctg tacgccttag acagcctcca
     4681 tcctgccgaa acaaaatacc gccaccttgc cgcccacttc atagaccgca gcaaaagcga
     4741 tgtgttgttc cgcttcctgc ccggcaatct ctcctccgac agcctgcgag tgctgaaaag
     4801 ccgcattgac cgcctctcgg ccgcgccaca gcgcacgccg ctcgacaatc gccagttggc
     4861 ctacgacctg gacgccattg aaaacttcac tttcaaccaa aaacaagctg cctatcggtt
     4921 tgccgatgtg cccgccctgc aaaacagctt agacgccgaa accgccgtgc tggaatacaa
     4981 aatctgcgcc gatgagacct ggctgctcta taccgaccaa caacagagcc ggctgcaccg
     5041 gatcggtatt tcggcaaacg agcttcggca actgacggat tcgctcgcgc tttttttaaa
     5101 caacgggaaa acattccata ttcctgatta tgagcgacat gcgacagctc tgtacgattt
     5161 cctgctccgg cccttcgagt cggaactccg gcgtcaccgg cgttgggcca ttgtccccga
     5221 tggcgttttg taccgtctgc cttttgaggc gctcatcacg cgggcttcgg gcgggccgcc
     5281 tgcatttgtc gtcaccgagt tcaaagattt ggccgtcaca tactctccgg cgctgaaaat
     5341 caggcagatg aatctggagc gaaaaaaggg cggcaagccc gccgccggcg cctcgtttta
     5401 caattacggc ccagacaaca acgacggcct ggtgtgttcc gaaaaagaat ggctggcctt
     5461 atccggtcgc gaccggcgcg cccgaaaata catgggcagc cgatgctcca agcaggcgtt
     5521 tattcaggac tggcaagccc ggcggcgcca taccgtgcat ttatcgctgc atggccaggc
     5581 cgatgccgcc gatcttctgg gcgctaagat atggtttggc ccgggcgcta ccgacgtcat
     5641 gtacggtttt gagatagccc acacgccggg ctatgccgag gaggtcattt tgtcggcctg
     5701 cgaaactgcc gacggcgcct gggctacagg agaaggcgtg tattctatgg cacggagttt
     5761 ttttcaggcg ggcgcgtgca gcgtaatggc cacgatctgg aaggtggaaa actgccacaa
     5821 tgcgcacatc ctcggctcgt tttatgcagc cccggcctcc ctgtctggcg cacaagcctt
     5881 ggccgatgcg aaacgcgcat acctccgctc gcaaaaggca taccagccgt attattgggc
     5941 gggtatggtg tgtatggagt gagagagcgg gatacttttt cagtgggtcg tatgaggnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttcaaaag ctacgagcta
     6061 tgagtcacga gccatgagcc tgcttgaccg gtagctactg tttgtacaca tcgtagagcc
     6121 tccatccctc aaaaaagtcc tcgaatcgtt gatagccttg aatcagagta ataactcctg
     6181 gaggtctttg agaagcatat ccactccatc ttcccagtcg ggcaattgtc gctaattacg
     6241 aaaaaggtac cacccgtttg caccaaaagt gtaccaccgc ccacgctgcc cgcgccgggc
     6301 agtttttttt cagccgtgta ttgctttttc gggatggagg gagcatcttt gctgnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgat gggcgcttta
     6421 agaataaggc cggatgataa agcggaaata cccacaccaa aggataagta aaaggcgagc
     6481 taaggttcat gtttttactt ggaggggggt gcggtaataa gagcgttggg cgaaatcatc
     6541 atcaaaaaat agataaatac acatggcaaa gaaaaatttc cttttcggtc tgttttctct
     6601 tttggttttg ttgggttgca atcataaccc cgtagcaagt tttacctttg aagtaaaaga
     6661 agatggcgaa gtagttttcg taaattcgtc agaaaactca aagtcttttc aatgggaatt
     6721 tggagatggg acatttgatg ttgcctctaa cccaactcac aaattcagtg cgaacggtaa
     6781 gtatgatgtt aaactaactg ccacgaacgg caaaggaagt cattcaagtg tggtaacctt
     6841 gaacctcgac aatgtcgccg ccgctaatta tgttttttgg accaagcatt caaattattt
     6901 accagttgaa atttatattg acggtgcact tcaaggcaat attcggaaat cattttcctc
     6961 tgcacttgat tgctttgccc cgggagcgct cacagtttca accacacccg ggaatcatag
     7021 atttcgtgca agaggcagaa atggaagcga gtggacagaa attgaactcg aattcgtaaa
     7081 aggagattgc aagtacctca gtctaaaccg aaccggcttg tcgaaatttt ctcgtaattt
     7141 tgcctgaaat tttaaaaaaa atggcaaaga aattagttcg gttcgattgg gcgataaagc
     7201 ggctgcttcg caataaggcg aacttcgaca tcctggaagg cttcctctcc gagttgcttc
     7261 acgaggattt taaaatcaag cnnnnnnnnn nnnnnnnnnn ncgaaggcaa caaggaaacc
     7321 gaggacgaca agttcaaccg ggtagatatt ctcgtggaaa ataacaaggg cgagttggtc
     7381 atcgttgaaa ttcagaacac ccgtgaagcg gactatttcc agcggatgtt atatggaacg
     7441 gccaaagtta ttgtcgaaca tatcgccgag ggacagccat attcccaagt caaaaaagtg
     7501 tactccgtga acatcgtgta ctttgacctc ggtcagggag acgactatgt ctatcacgga
     7561 acgactacat ttatcggcat ccatagcaac catgaactgg aactgtcgga aaaacagaag
     7621 gaacttttca aaaaaacatc ggtatcggac ttgtacccgg agcactatgt gataaaaacc
     7681 aatcggttca acgaagtcgc caaggacact ttggacgaat ggatatactt tctgaaaacg
     7741 gcagaaatca aggatgaatt cacagccaaa gggttgaaag cggccagcca aaaacttgac
     7801 atccttaaat tagagcctgc tgaccgcaag gaatatgata ggtacatgga ggcacagcgg
     7861 agcgatgcga gttttgtgga gactgtaaaa ctggaaatta catcggcggt gaagagcaga
     7921 gaagaagaaa ttgctgaaag tgccctaacc gaaggtgcat ctgccgattt tgttgttaaa
     7981 atcacaggac tttctatcga ggaagttagg agcattgctg ataggcttgg cttgaataaa
     8041 taaatcgccc aacaatcact tcccgaaaat cgctcctaag cgtcgtgcat tcgggaagcg
     8101 taacgttgca aaaaacatca ccacctgact gcaacaacaa aataacagtt aatggcagaa
     8161 ataaaactat ataaaacaac cataaaagga ctgaaaatta ttggtatgtg tttaccattc
     8221 gcnnnnnnnn nnttggtcta tggatgataa cggacaatcc ttatggaagt accgaatata
     8281 tgatgggctg ggttagtact tgtttttttg ggcttgggat tccaattgga ctttttcaga
     8341 catttgacaa aagacctcag attgttatca cggaaaacgg catttgggac aggacaacac
     8401 atcaggacga aataaaatgg gaacaaataa ttgaagccta tccgattgat attcacggac
     8461 aaaagtttat ttcattagta acagacaaca cctttgtttt caagaagaaa acttccaggc
     8521 gggctgcggc aatcaatcaa tttattggag cacaaaaatt aaaccttcgt ttaggacaaa
     8581 tcaatattga cgaaattgga ttgaccaatt tcatcaatca aatcaccaaa gagacaatag
     8641 aagagcgaag aaggtcaatt aagaatttta agattaaaga aacccgtttc acatattcag
     8701 acttacagaa aatctttctt tatatcttcc tctctgttgc gcttttagtt ttgacacttt
     8761 caagctatgt ggtattttgg attgtaatgg gcataatggg attttcagca attacggcac
     8821 gatggaaacc agataatttg actattagaa agtatgcgtg gattgggact tggcttggac
     8881 ttgtaaatat ggttttagta ttgggagcga ttaaaattta tgacaacgta actgaaaacg
     8941 tgggagaaca attagcgata gaaattgaag aatttcacag cctaaactca acttacccga
     9001 aagagataaa atcccttact cgaaacttgg aattcaattt tttagaacgt ttttttgctg
     9061 atcaaacgca ccagatggag gatcgcgttg tacctcttcn nnnnnnnnnt ttgctgatca
     9121 aattgaatat aaatctatcg gcaacgatta cgaattagaa gcaactatga tgtttgggaa
     9181 tctgagaaaa tacgataaat acaatggcca atggagataa atactacccg ctcgctccaa
     9241 gactccatct tcatgagcca ttccgccgcc gcccgctgaa ccgcctgttg ctctatctcg
     9301 ttaactaatc gtccccgctc ccaagcccgg cgcaccattt accaacccaa atcaatccaa
     9361 ttctgccatg ttcagcaaac aacagcgcaa gattaaaaga atcagcgagt tcgtagccat
     9421 tctttcaaag tacggattta aagacatttt ggcccggatg aatatcggcg cccctgctgc
     9481 cgctgagccg cttgtggccg attctgaata cagtagaatc cggaaagttt tggaagaatt
     9541 ggggcctact tttatcaagc tggggcaaac attgagcaac agagatgatt tattgccgaa
     9601 agcattgatt gaggaattgc aacgcctgca ggacagggtt gaggtcctcc ccatgaacat
     9661 acatgaggtg ctggaggata atttcggccc tgattataaa gactcgtttc agcacatcca
     9721 taccgaagcc ctggcggctg catctattgc ccaaatatac cgggctgttt tgtccggcgg
     9781 cgagcaagtg gttttaaagg tgaaacaccc aagcgtagag cataccattg agacggactt
     9841 gctgctgatg aaagatgtgg cacgggtatt gacccaatat tttgaatttg cagaaaaaat
     9901 aaatttgatg caggcgttca gcgcctttga aaaatcgctc cttgccgagt tgtcgtttgt
     9961 caatgagcgg aaaaatatag aacggttggc ccagaatttt aagggcgatg caaatgctta
    10021 tgtgccaaaa gtttatcctc atttatcgaa caacgaagtg ctgtgcatgg agtttattga
    10081 cggcgctaaa attacagaca acgggtatca cgaccggcat ggactggatg ttcgacagct
    10141 tgcccaaaat ggtttggaca tttatttaaa acaaattttg gagcacggct tttttcatgc
    10201 cgatcctcat gcgggcaata ttatgacgct gagcgacggg cgcattgctt ttatagacct
    10261 gggcgcaatg ggtgaaattt atcagtcgga tcgggtcatt ctggaagatt tgattttgca
    10321 tttggccgct caaaatccgg caaaattggt tgaaatatta aaaagaatgg ccctgcgcat
    10381 ggatgttcct gacgaaagaa aactgcacaa cgatattgcc gatgttttga acatggtcaa
    10441 tacaagctcg ctggaggact taaaaatcac ggtattgctg gataaattca aggaaattct
    10501 ttttgaaaat aaaatcatta tgcccgatta ttttatgctg ttggtgcggg gcatcatact
    10561 catagaaagc gttgggcgca cgttgaatcc cgatatcaat attgtaaaaa gtatggaacc
    10621 ctatatcgcg ggcgttcttc aaaagcgatt cagcccggat tatttgtacc ggcagggcat
    10681 tgcgaaactg agcgaattgg gcgacgattt gcaaaatgtg ccggttgaag tgcgacaaat
    10741 tttgaaaaaa ttacatgatg gaaatctgag tgttcaggtc aaaaacgatg aaacccgcca
    10801 actcaacgca acggtagaga aagggctggc caatctggtg atggccgttg ttttgtctgc
    10861 gctcctgctc tcgctgacac tcctcttagt gtcgaatacc cgcctcacat tgtcggcctg
    10921 gtcggttccg gcggttttgg tgttgtgctg ctccgtcgta ctatccgggt tgctggcaat
    10981 caggctattg cgtcggtagc gatgtttttt gtgactattc agc
//
LOCUS       DAORLF010000109        10980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3203_length_10980_cov_1.849611, whole genome shotgun sequence.
ACCESSION   DAORLF010000109 DAORLF010000000
VERSION     DAORLF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_10980_cov_1.849611"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PK198_10460"
     CDS             complement(<1..1712)
                     /locus_tag="PK198_10460"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF39202.1"
                     /translation="MRVLRYTLFLLLAPILTWSQYEGNFPPPDTPAVIRAVKAVGKIA
                     LDGVPDETDWLNAPPIKDFFRVEPKQGGAYQFTTEMRVLFDDKHLYVSVFCTDSLGKK
                     GIRVQDFRRDFRFGENDIFFFQLDPQNLRRYCVSFQTTPIGTQRDAQVFDDNIVDNDW
                     DALWAVKTHIAQNGWSAEFSIPFKSLRYELPPDGQTASWGFSASRLARRTYEQTVFPP
                     VPQSFSPYRMTYAARLEGLELPKPALNLRINPYALYNFEKRGDEPDAGTAKIGADLKW
                     AVNSHAVLDMTLNTDFAQADVDRAVNNLTRFNVFFPERRQFFLENSGIFPSDGSSIYP
                     FFSRTIGLANSQFNANPVPIDIGFRWNDRNEKRTLSALYVHQRATPNQGGANFSLARY
                     QKNLGQQNNIGLLFTHRYDEKSDLLSDNHNSTLTIDGLLRPNDTWTVKWMLSGSYDEN
                     SGQVGHAGNLFTGIQRNNMYAGWVTKWVSRHYQPGMGFVAANNFINHNPGGYFIWRPK
                     KEKSLIRRADPGLFVDYFQNASDGKLQQLELFIFPVYLWLRDNTFIRAEVVPTWQRID
                     FEFAP"
     gene            complement(1739..3163)
                     /locus_tag="PK198_10465"
     CDS             complement(1739..3163)
                     /locus_tag="PK198_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF39203.1"
                     /translation="MFLLQLQYEWKHLSRNGAAWLLLLFLGGAMVFGAYNGRQRAARQ
                     KQAVQALKTAQDEKFAALEAEADSIVKGLKKMEQWWLDPTNPLTVGLLRKGGGTLVIE
                     PAPGQALAVGMSDLQPEAYAMLFGGGAARGSSDYDNPARLALGAFDLAFVIVYLLPLF
                     VIALTYNLLSAEREQGTLTLLLSQPVQGRRIFVSKMLARFVVLSLMATLLFVPSAIWA
                     GIGAGLTAQATGATLLYGFFWFLMALAVNMLQQNSAFNALACIGAWLLLVVVMPALVN
                     MLAEKVHPVPSRAGYENALRTADIEFEKMKPAILDTFYDANPALERKPDDAKSPRDWW
                     LEELYLLDIRKAHVADIKALYEDKSQSHADFARKLTVLSPALVLHDYLTGLAGTNRDA
                     QLATQEVLESKAQTWLDWFAAKYAADAKLQAADYAAIRSMLEPVQPKQSEGSSNGLLW
                     SVIYLTLMALPLILGRRRQMMAAI"
     gene            complement(3166..>3649)
                     /locus_tag="PK198_10470"
     CDS             complement(3166..>3649)
                     /locus_tag="PK198_10470"
                     /inference="COORDINATES: protein motif:HMM:NF023464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3526 domain-containing protein"
                     /protein_id="HRF39204.1"
                     /translation="VSTVDSLPFNWDGFIMQKGEEHETYVFQKHKDALLAIYQQQQNL
                     LEASALLSPYLLVNLSSQRLAGTDVHTFHNFLAAAEKYRVALVGELNNDLMLNFKYGD
                     WDGKRDKAFFASNKKFEYRGPAFREVWQISRPAMALLFGWYLLSGLLGLFVFQRIKPI
                     "
     assembly_gap    3650..3786
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3787..>4058)
                     /locus_tag="PK198_10475"
     CDS             complement(<3787..>4058)
                     /locus_tag="PK198_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39205.1"
                     /translation="GVVVLSLALPFLLLGGLGLAAPGAGAEDWQRYGLMSAGYLIYLG
                     CFIHLTLIVSAFAQRANVALVALLGIWIAMCLISPKAVADFAAWSH"
     assembly_gap    4059..4203
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4204..4577)
                     /locus_tag="PK198_10480"
     CDS             complement(<4204..4577)
                     /locus_tag="PK198_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39206.1"
                     /translation="MKAIIIARQELRSIFRERRFQAAAAILSILFALSVFGAWDYYAS
                     NEKQHAEAARTARAHWDNQEDKNPHSAAHYGTFVFKPVYPLSFFDRGIDAFTGNTLFL
                     EGHVMHEAKHRAVADRSELSRLG"
     gene            complement(4671..4961)
                     /locus_tag="PK198_10485"
     CDS             complement(4671..4961)
                     /locus_tag="PK198_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39207.1"
                     /translation="MRQKIGIAIAMAKKAKALFLDEPTSGLDPKASNDFSTLLKELAK
                     EGVAVLMATHDIFRAREVATRIGIMREGKLVSVHQGDALTANELEKIYLETV"
     gene            complement(4934..5362)
                     /locus_tag="PK198_10490"
     CDS             complement(4934..5362)
                     /locus_tag="PK198_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39208.1"
                     /translation="MISARHLTKKYGDHTALDNLTLDIQPGEIFCLLGQNGAGKTTTI
                     NCFLGFTQPSSGQAWIHGEDVVQAPVTTKKHLAYLPEVVMLYPNLSGIENLEYFSRLA
                     GFRYERGELLAFLEAAGTNRSGNAGFLPIPKGCARKSALP"
     gene            5548..6234
                     /locus_tag="PK198_10495"
     CDS             5548..6234
                     /locus_tag="PK198_10495"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRF39209.1"
                     /translation="MQEFLSLYSKMENPTLEMVLFTFLLAFLCSVAIGMTYYFTTPTT
                     LKTANYIQALILSSVIATMILQSIGDNVASGLGIIGALTIINFRTAFRDPRDIIFMFA
                     ALGVGIACGSYVFVIAAAGTAMFCTLALVLKFTPFSLGNHQIVELRVRIKDQETAAPV
                     EEILTRYCQKFTLDGFRNNSTEGVAFQDRDYVIVFRNDQEHAEMVRAIEAAGGEVRKL
                     NNQNSEATEV"
     gene            6451..6678
                     /locus_tag="PK198_10500"
     CDS             6451..6678
                     /locus_tag="PK198_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39210.1"
                     /translation="MVRIELDVRNDQFESFVQLLKTLNYVSIVHMEAGDKESISPFYW
                     LELLASAGGIQSIENASEWQRLSRGDRERIA"
     gene            6730..>7076
                     /locus_tag="PK198_10505"
     CDS             6730..>7076
                     /locus_tag="PK198_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39211.1"
                     /translation="MKINLLYLAIPAAILACVYIVRDLQGQSEQTFFGSAETEPALLH
                     VEEDVLVQEVFTAVGSQVKQGDTLALLYRMELDRELAEIASDQRRISSDLVSSQELLR
                     QERATIQAKAAAQ"
     assembly_gap    7077..7124
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7125..7730
                     /locus_tag="PK198_10510"
     CDS             <7125..7730
                     /locus_tag="PK198_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF39212.1"
                     /translation="IDERVKSAVYAGLKTDRTASKVAIAGLRREIELVGEVMQQELNE
                     LAVEEKSSARLYQTESKNATQRIDIQDAERRRLVLIAPVDGYVDQVSISAGTPMPAFR
                     DMMRIYPLRPAKVIGFIHETADIPFRIGDSVSLISGVRPDKLVRGQITAAGPKLVELP
                     LRLRKFAEVRAWGREVIISLPPDNPYYIGERITVSLQTAEQ"
     gene            7727..8998
                     /locus_tag="PK198_10515"
     CDS             7727..8998
                     /locus_tag="PK198_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39213.1"
                     /translation="MRPGLVVLAGGLSAQPLRIAEVLAGVDKDLRVRHSQELADLAAS
                     LRYHIPLLKEVEARIGIRGSVLGDTIYGYLRNEDIYALQIETNSFKEIRAQKNWRQAQ
                     INTLLAEKELYREEALLARYEVCAAWYHASRRQVAQNRLLELLQQKQQLLRISAAEGL
                     DFSIKDAISVEEDLLDLQNKLAETAHVLEENQLKIRQFMGLTDNMELDTAHFITADEI
                     AAYLVQSGADASPTAAIQLRSSISDLSEAAYQNANAQNRQIFNSLRLNYDHPLYLERP
                     SRFNTFNNFSIRLGLRIPLTGNNNFRSSRAMLEWREDELAAQRTAEMLAAERDRQRLQ
                     LAQRIGAYRNYLDQEQQSLTARLLSDAALRPNLSAKELTDMEIIQQKLRLRHIAAAAE
                     LTQTYLQLLLLDGKMNAGERRNWLSRVGEEW"
     gene            9109..9534
                     /locus_tag="PK198_10520"
     CDS             9109..9534
                     /locus_tag="PK198_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39214.1"
                     /translation="MLTRLPLCCILAAFLCACATRSSEPAVPYYTMTDAENIADFEGR
                     KVRFLCTLAEIPMAHMMRPSLNHSGKEEHIYIDPLPKYGFGQIVAYFKAASFQRNMMK
                     PNRTYWVSGTVGSVSGAGMGGGTHTEYYLELEGAELVRE"
     gene            complement(9595..>10339)
                     /locus_tag="PK198_10525"
     CDS             complement(9595..>10339)
                     /locus_tag="PK198_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF39215.1"
                     /translation="NVLNPFNNQLSQLSFEKGNPFLRPEIVNNLELGYTWKYRYNFKL
                     GYSRTIDQITRLIGPDPVDPRASYISWDNLATQTILSFSASLPVQVSKKWNAYFSLSA
                     SHLDNQADYGGDAVVDVQAFTYNIYQQQTFDLPAGIKFEVSGWFSGPGVWGGVFEYET
                     SWSLDLGLQRKFLKDRLTLRLGASDIFFESGWDGVSKFNGMVSEGGGRWDSRRVSLSA
                     NYRFGNDNVKSRNRKTGLEEEGRRVGSGG"
     assembly_gap    10340..10370
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10371..10901)
                     /locus_tag="PK198_10530"
     CDS             complement(<10371..10901)
                     /locus_tag="PK198_10530"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39216.1"
                     /translation="MREYIDNRVAAGNYGNTSIRLQPNQYFNAGEDILLTEIVNSFDT
                     PTDIKIYTAKADYEDDMLGGKLGFGAKYSKVVSDNTFLFFDVVNGLNLRNDRLSNRFE
                     YNENVYAAYVNYSRSLGKKWKFSAGLRAEQTDAMGNLQAFLPELQAPPVELNYLSWFP
                     SAGLTWQVAQMHSLALN"
BASE COUNT         2463 a         2724 c         2852 g         2580 t
ORIGIN      
        1 ggtgcaaact caaagtcaat gcgctgccag gtaggaacca cttccgcgcg gatgaacgta
       61 ttgtcgcgca gccagaggta aacgggaaaa atgaaaagtt ccaattgttg caatttgccg
      121 tcggaggcat tttggaaata atcaacaaac aatcccggat cggcccgccg gatgagtgat
      181 ttttcctttt tgggcctcca gatgaaatag ccccccggat tgtggttgat gaaattgttg
      241 gcagcaacga atcccattcc gggctggtaa tggcgcgaaa cccacttggt gacccagcct
      301 gcgtacatat tgttgcgctg tattccagta aatagattac ctgcgtgccc cacttgccca
      361 ctattttcat cgtagctgcc gctcaacatc catttcacgg tccaggtgtc gttggggcgc
      421 aacaggccgt ctatggtcag tgtcgaattg tggttgtctg aaaggagatc gcttttttcg
      481 tcataacgat gggtgaacag caagccgatg ttgttttgtt ggcccagatt cttctgatat
      541 ctggccagcg agaaattagc gcctccttga ttgggcgtag cgcgctggtg tacatagagt
      601 gctgataatg tgcgtttttc attgcggtcg ttccatctga aaccaatatc aatgggtaca
      661 gggttggcat tgaactggct gttggccaag ccgatggttc tgctgaaaaa cggataaata
      721 cttgagccat cggaggggaa gatgccgctg ttttctaaaa aaaattgtcg cctttcggga
      781 aagaacacat tgaaacgggt gaggttgttc actgcgcggt ccacgtcggc ctgagcaaaa
      841 tcggtgttga gcgtcatgtc caaaacagcg tgggaattga cggcccactt caggtcggcg
      901 ccgattttgg ccgtgcctgc atctggttca tcgccgcgtt tttcaaaatt gtagagtgca
      961 taaggattga tgcgcaggtt gagcgcgggt ttgggcagtt ccaagccttc gaggcgggcg
     1021 gcataggtca ttcggtaagg agagaaagac tgcggcaccg gcggaaaaac agtttgctca
     1081 taggtgcgtc gtgccaatcg agaggccgag aacccccagg atgccgtttg gccatcgggc
     1141 ggcaattcgt acctgaggct tttgaacgga atgctgaact ctgccgacca gccgttttgt
     1201 gcaatatgcg ttttgacggc ccagagcgca tcccaatcat tgtcaacgat attgtcgtca
     1261 aacacctgcg cgtctctttg cgtaccgatg ggcgtggttt ggaaagatac acagtaacgg
     1321 cgtaggttct gcggatcgag ttgaaagaaa aaaatgtcgt tttctccaaa tctgaaatcg
     1381 cgccggaagt cttgcactct gatgcctttt ttacctaagc tgtcggtgca aaaaacgctc
     1441 acatacaagt gtttatcatc aaacaagacg cgcatttcag ttgtaaactg ataagctccg
     1501 ccttgcttgg gttcgactcg aaaaaagtct ttgatcggag gggcgttcag ccaatcggtt
     1561 tcatccggca cgccatctaa tgcaattttt ccgacggcct tcacagcacg aatgacggcc
     1621 ggcgtgtcag gtggcggaaa attcccctcg tactgactcc aggtgagaat aggcgccaac
     1681 agtaaaaaaa gggtgtatct cagtactcgc atgggtgatc gttgtacctt gctgttcatt
     1741 atattgccgc catcatctgc cgccgcctcc ccagtatcag aggcaatgcc atcagcgtca
     1801 gatatatgac agaccagagt aacccatttg aactcccttc actttgcttg ggttgcacag
     1861 gttcgagcat gctgcgaatg gcggcataat cggctgcctg aagttttgca tcggcagcgt
     1921 attttgcggc aaaccagtct agccaagtct gggctttgga ttccaacact tcctgcgttg
     1981 ccaactgcgc gtccctgttg gtgccggcca gtccggtcag atagtcgtgc agtaccaagg
     2041 ccggagacag caccgtgagt ttacgggcaa aatcggcgtg gctttgtgac ttgtcttcgt
     2101 acaatgcctt gatgtcggct acatgcgcct tgcgtatatc taaaaggtac agttcctcca
     2161 accaccagtc acggggcgat ttggcgtcgt cgggtttgcg ttccaatgca ggattagcgt
     2221 cgtagaaggt atccagaata gcaggtttca ttttttcaaa ttcgatatcg gctgtgcgca
     2281 gggcattttc gtaaccggct ctggagggaa cgggatgtac tttttctgcc aacatattga
     2341 ccaaagccgg catgaccacc accagcaaca accacgctcc gatgcaggcc aatgcgttga
     2401 aggcgctgtt ttgttgaagc atgttgacgg ctaaggccat tagaaaccaa aaaaatccat
     2461 acaataaggt agcgcccgta gcttgtgctg taagacccgc gccaatgccg gcccagattg
     2521 ccgacggtac aaaaagcaag gtggccatca aagacaacac gacgaaacga gccagcattt
     2581 tggatacaaa aatgcgcctg ccctgcacag gctgcgataa caataaagta agggtgccct
     2641 gctctcgctc ggcagataaa aggttgtatg tgagggcgat gacaaaaagc ggcaagagat
     2701 atacgatgac aaaagccaga tcgaaagcgc ccaatgccag ccgcgcagga ttgtcgtagt
     2761 cgctgctgcc gcgtgccgcg ccgccgccga acagcatggc gtatgcttcg ggttgcaagt
     2821 cgctcatgcc tacggcgagg gcctgtcccg gcgccggttc gatgacgagg gtgccgccgc
     2881 ctttgcgcag cagtcccacc gtcagcgggt tggtgggatc gagccaccat tgctccatct
     2941 ttttcagtcc ttttacaatg ctgtcggctt cggcttcgag ggctgcaaac ttctcatcct
     3001 gagccgtttt cagggcctga accgcctgct tttgccgggc tgcccgctgc cggccgttgt
     3061 acgcgccaaa caccatagcg cctccgagaa agagcagcaa caaccaggcc gcgccgttgc
     3121 gggataagtg tttccactcg tattgcaatt gaagtaaaaa catggttaaa tgggtttgat
     3181 gcgttggaaa acaaacagtc cgagcagccc gctgagcagg taccagccga acaacaaggc
     3241 cattgccggg cgactgattt gccagacctc cctgaaagcc gggcctcggt attcaaactt
     3301 cttgttggaa gcaaaaaatg ccttgtctcg cttgccgtcc caatcgccgt atttgaaatt
     3361 cagcatgagg tcattgttta gttcgcctac caatgctacc cggtattttt cggctgcggc
     3421 cagaaaatta tggaaagtat gcacgtcggt gccggccaag cgttgcgaac tgaggttgac
     3481 caacaaatac ggcgagagca gagcgcttgc ctccagaagg ttttgttgtt gctgataaat
     3541 ggccagaagg gcgtctttgt gcttttgaaa tacatacgtt tcgtgttctt ctcctttttg
     3601 catgatgaag ccgtcccagt tgaaaggcag gctgtccact gtactcactn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnatgg ctccaggctg caaaatcggc cactgctttg ggggatatga gacacattgc
     3841 tatccaaatg ccgagcagcg ctaccaatgc cacattggct ctttgggcga aagccgaaac
     3901 gatcaaagtc agatgaatga aacagcccag atagatcaaa tagcctgccg acatcaggcc
     3961 gtaacgctgc caatcctcag caccggcgcc cggcgctgcc agtcccaagc ctccgagtaa
     4021 caaaaacggc agtgcaagac tcagcaccac cacgcccann nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnccgaggc gggacagttc gctgcggtcg gctacggcgc gatgcttagc ctcgtgcatg
     4261 acgtggccct ccaagaacag cgtgttgccg gtgaaggcgt ctatgccccg gtcgaaaaag
     4321 gaaagcgggt aaacgggttt gaaaacaaaa gtgccgtagt gcgctgcgct gtgcgggttt
     4381 ttatcctcct gattgtccca atgcgcgcgg gcagtgcgtg cggcctcggc atgttgtttt
     4441 tcgttggagg cgtaataatc ccaggcgccg aacacagaaa gtgcaaagag tatgctgagt
     4501 atggcggcag ctgcctgaaa tcgccgttcg cggaagatgc tgcgcaactc ttgccgggca
     4561 atgatgatgg ctttcataac ttaagtgatt gggctaaatg aattcatgct attttgcttg
     4621 ctcttgattg gtaatcagca catttacaca ttaccaaatc agcatatttt ttatacggtt
     4681 tcaaggtaaa ttttttccaa ctcattggcc gtcaaggcat ccccctgatg caccgacacc
     4741 aattttcctt cccgcatgat cccgattcgg gtggctactt ctctggcacg gaaaatgtcg
     4801 tgggtagcca tcagcaccgc tacgccttct ttggccaatt ctttgagcag cgtagaaaag
     4861 tcgttgctgg ctttggggtc taaaccgctg gtcggctcgt ccaaaaatag ggctttcgcc
     4921 ttcttggcca tggctatggc aatgccgatt ttctggcgca tccctttgga ataggtagaa
     4981 agcctgcgtt gcccgatctg ttcgttccgg cggcctccaa aaaggcaagc aactcgccgc
     5041 gctcgtatct gaatcccgcc aggcgactga aatattcaag gttttctatg ccgctcaggt
     5101 tggggtagag catgaccact tcgggtagat aggctaagtg ttttttggtg gtaaccggag
     5161 cttgaaccac atcttcaccg tgaatccatg cctgcccgga actgggctgt gtgaatccca
     5221 gaaagcaatt aatggtagtg gtttttcctg cgccgttctg ccccagcagg caaaatattt
     5281 ctccgggttg gatgtccagc gtgaggttgt ccaaggcggt atgatcgccg tattttttgg
     5341 tgaggtgcct ggcagaaatc ataagttgcg gttttgcaaa tgaaacaatg ttgcaaatgt
     5401 actattctct ttgatatatg caacaatgat gcaaataact tttcaattca gccgcccatt
     5461 ttaccgttca ggcaactttt ccccacccct tttccccatt atgtttagtt ttggccgctt
     5521 tgaacaaatc agcgacttgt aatcaatatg caggaatttc tctctttgta ctccaaaatg
     5581 gaaaatccca cgctggagat ggtgttgttc acgttcctcc tggcgtttct gtgttccgtg
     5641 gccatcggca tgacctacta tttcactacg ccgaccacgc tcaaaacggc caattacatt
     5701 caggcgctga tactcagttc ggtcattgcc accatgattt tgcagagcat cggcgacaac
     5761 gtggcttccg gtctgggcat catcggagcg ctcaccatca tcaatttccg cacggctttc
     5821 cgcgatcccc gcgacatcat cttcatgttt gcggccctcg gcgtgggcat tgcctgcggt
     5881 tcgtatgtat ttgttattgc ggcggcgggc acggccatgt tctgcacgct ggctttggtg
     5941 ctcaagttca cgccattttc gttgggcaac caccagatcg tagagttgcg ggtgcgcatc
     6001 aaagatcagg aaactgcagc gcccgttgag gagattctga cgcgctactg ccagaaattt
     6061 accctcgacg gctttcgcaa caacagcacg gagggggtgg cgtttcagga tcgcgattat
     6121 gtcatcgttt ttcgcaacga tcaggaacac gccgaaatgg tgcgcgccat agaagctgcc
     6181 ggcggagagg tgcgcaagtt gaataatcag aattcggaag cgacggaggt ttgagggttg
     6241 acttattatt aggtatgtca cacaagcttc gagccgcgac cacgccttgc gtggttgctt
     6301 ttttgttaag gaattagatt tttcttatgg ggaaattcta aatgcaccat caaaaatgag
     6361 tacttttctt catttaggaa atcaggctcg tgctttgtcg ggcatatcac ctctttttcg
     6421 catgttgcct aataattaca tttgtctaat atggttagaa tagaacttga tgtcaggaac
     6481 gatcaatttg agtcgttcgt ccaacttttg aaaacgctga attatgtgag tatcgtccac
     6541 atggaggctg gggataaaga aagcatttct ccgttttatt ggttggaatt gctggcttcg
     6601 gcaggaggaa ttcaaagcat cgagaatgct tccgagtggc agcgtctgtc aaggggagat
     6661 agagaacgta ttgcgtaatg ttctgtatcc agtacgagtt aattttgata ttgaaccgca
     6721 tttaattaaa tgaaaatcaa cctcctatat ttagccatcc ccgcagccat tctcgcctgc
     6781 gtttacatcg tccgggactt gcaaggccag agcgagcaaa ctttttttgg ttctgccgag
     6841 accgaaccgg cgcttttgca tgtcgaagaa gacgtattgg tgcaggaggt gttcaccgct
     6901 gtgggcagtc aggtaaaaca aggcgacacc ctggcgctgc tgtaccggat ggaactcgac
     6961 cgagaactgg ccgaaatcgc ttcagatcaa cgcagaatat cttccgatct cgtatcctcg
     7021 caggaactgc tccggcagga acgcgccacc attcaggcga aagccgccgc ccagatnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatagac gagcgcgtca
     7141 aatcggctgt gtatgccggt ctgaaaacgg accgaacggc gtctaaagtc gctattgccg
     7201 ggcttcgcag agaaatcgaa ttagtcgggg aagtgatgca acaagagttg aacgaactcg
     7261 ccgtcgaaga aaaaagcagt gcgagactgt atcaaaccga aagtaaaaat gctacacaac
     7321 gtattgatat acaagatgct gaacgccgcc gcttagtact catcgctccg gtggacggct
     7381 atgtggatca ggtgagcatc agcgccggaa cacccatgcc tgccttccgc gatatgatgc
     7441 gcatctaccc gctgcgcccg gccaaagtca tcggttttat tcacgaaact gccgacatac
     7501 ccttccgcat cggcgattcg gtcagtttga tttccggggt gcgccccgac aaattggtgc
     7561 gcggacagat caccgccgcc ggcccgaaat tggtggaatt gccactcaga ctccgaaagt
     7621 ttgccgaggt gcgcgcctgg ggccgcgagg tcatcatcag cctgccgccc gacaacccgt
     7681 actacatcgg cgagcgcatc accgtctcac ttcaaacggc ggaacaatga ggccggggct
     7741 tgttgttttg gctggaggtc tgagcgcgca acccttgcgt atagccgagg tgctggcggg
     7801 cgtggacaaa gacctgcgcg ttcgccacag tcaggaattg gccgatctgg cggcctctct
     7861 gcgttatcac atccctttgc tcaaagaagt ggaggcccgc atcggcattc gcggcagtgt
     7921 gctgggcgat acgatttacg gctacctgcg caatgaagac atctacgcgc ttcagatcga
     7981 aaccaatagt tttaaagaaa tcagagcgca gaaaaactgg cgtcaggcgc aaatcaacac
     8041 cctgctggcc gagaaagaac tgtaccgcga agaagcgctg ctggcccgct acgaagtctg
     8101 tgcggcctgg tatcatgcga gccggcggca ggtggctcaa aaccggctcc tggaattgct
     8161 gcaacaaaaa cagcagttgc tgcgcatctc ggcggcggaa gggctggatt tcagcatcaa
     8221 agacgccatt tcggtagagg aagacctgct cgacctccaa aacaaactgg ctgaaacggc
     8281 ccatgtattg gaagaaaatc aattgaaaat caggcagttt atgggcctga cagacaatat
     8341 ggagttggat acggcacatt ttatcactgc cgatgaaata gccgcttatc tggtacaatc
     8401 aggcgccgat gcctcgccga cggccgccat tcagttgcgt tcctccatca gcgatctttc
     8461 ggaggcggct tatcaaaatg ccaatgccca gaaccgacag attttcaatt cgttgcgtct
     8521 caattacgac catcctctgt atttggagcg cccctcccgg ttcaatacct tcaacaactt
     8581 ttccatccgc ctgggactga gaattccact cacgggaaat aacaattttc gcagcagccg
     8641 ggccatgctc gaatggcggg aggacgaatt ggcggcccaa cgaaccgctg aaatgctggc
     8701 tgctgaacgc gaccggcagc gtcttcagtt ggcccaacgc atcggagcct accgcaacta
     8761 cctcgatcag gagcagcaaa gtctcaccgc ccgcctgctt tccgatgccg ctcttcgccc
     8821 caacctcagc gccaaagaac tcaccgatat ggaaatcatt cagcaaaaac tccggcttcg
     8881 acatatcgcc gctgcggcgg aattgacaca aacatactta caattgctgc tcttggatgg
     8941 caagatgaat gccggcgagc gcaggaactg gctgagtaga gtgggggagg agtggtgagt
     9001 tttttaaacg tatctatttt gtttttccaa cagccgcgag ctacgagttt gtttattgtt
     9061 aggaatttga ccttcctcta tgatttttat tcaaaaatac ctgttgccat gcttacaaga
     9121 ttaccccttt gctgcatact ggcggcgttt ttatgcgctt gtgccacacg cagttctgaa
     9181 ccggcagtgc cgtactacac catgacggac gctgaaaaca ttgccgactt tgaaggccga
     9241 aaggtcagat ttttgtgtac ccttgcggaa ataccgatgg cgcacatgat gcggccttct
     9301 ttgaatcact ccggcaaaga agagcatatc tatattgatc cgcttccgaa atatggtttt
     9361 ggacagattg tggcgtattt caaggcagca tcgttccaac gaaacatgat gaagcccaac
     9421 cggacttatt gggtgtcggg cacggtaggt tcagtttcag gagccggcat gggcggcgga
     9481 acgcacacgg agtattatct ggagttggag ggggcagagc ttgttcggga gtaatccgtt
     9541 gaaggttttg ccgctcccgt ttggaaaata tagaggcggc aaaaccttca ggcatcaccc
     9601 accgctgccc acccggcgac cttcttcctc caaccccgtc ttgcggtttc tcgatttcac
     9661 gttgtcgttg ccaaaccggt aattggcact gaggctcacc cggcggctgt cccagcggcc
     9721 accgccttcc gacaccatgc cgttgaattt ggaaacgccg tcccagcccg attcgaagaa
     9781 aatgtcggag gcgcctaagc gcagggtcag gcggtctttc aggaacttgc gttgcaaacc
     9841 caggtccaag ctccaactgg tctcgtactc gaacacacca ccccaaacgc cggggccgga
     9901 gaaccagccc gatacctcga acttgatgcc cgccggaagg tcgaatgttt gctgctgata
     9961 gatgttgtac gtgaatgcct gcacatccac cactgcgtcg ccgccgtagt cggcctgatt
    10021 gtcgaggtgc gatgcgctga ggctgaaata ggcattccat tttttgctga cctgcaccgg
    10081 caaactggcg ctgaagctca ggatggtttg cgtggcgagg ttgtcccaac tgatgtagct
    10141 ggctcgcgga tccaccgggt cggggccgat gagccgggtg atttggtcta tggtgcggct
    10201 gtagccgagc ttgaagttat accggtattt ccaggtgtag ccgagttcca ggttgttcac
    10261 gatttcgggc cggaggaagg ggttgccttt ctcgaaggaa agctggctga gttggttgtt
    10321 gaacggattg agcacgttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attgagggcc
    10381 aggctgtgca tctgcgctac ctgccaggta aggccggcac tgggaaacca gctcaagtag
    10441 ttgagttcca cgggcggcgc ttgcaattca ggcaaaaagg cttgcaggtt gcccatagca
    10501 tccgtttgct cggcgcgcag gccggcgctg aacttccatt tttttcccaa ggatcgggaa
    10561 taattcacat acgcggcata cacgttttca ttgtattcaa agcgattgga taggcggtcg
    10621 ttgcgcaggt tgaggccgtt gactacatcg aaaaacagga atgtattgtc agaaacgacc
    10681 ttgctgtact tggcgccgaa gcccaatttg ccacccagca tatcgtcttc gtaatctgct
    10741 ttggcggtat aaattttgat gtcggtaggc gtatcgaagc tgttgacgat ttcggtcagc
    10801 agtatgtctt ccccggcgtt aaagtactga ttgggttgca gtcgaatgct ggtgttgccg
    10861 tagttgccgg cagcgacgcg gttgtcgatg tactcgcgca tcgactcggg caacgcaaag
    10921 ctcatggttt ggcggctcat gcaggcatct tggtcggttt gacaacggtt gtcaacttcg
//
LOCUS       DAORLF010000110        10957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3213_length_10957_cov_2.074665, whole genome shotgun sequence.
ACCESSION   DAORLF010000110 DAORLF010000000
VERSION     DAORLF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_10957_cov_2.074665"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>246)
                     /locus_tag="PK198_10535"
     CDS             complement(<1..>246)
                     /locus_tag="PK198_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRF39217.1"
                     /translation="VENIRDVMAIEDTQQYRGLYHVLGGVISPIEGIGPEQLHIDDLI
                     QRVAGGEVKEVIMAISPTIEGDTTIYFISKKMQGMEVQ"
     assembly_gap    247..433
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(511..1833)
                     /locus_tag="PK198_10540"
     CDS             complement(511..1833)
                     /locus_tag="PK198_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF39218.1"
                     /translation="MKNTLPVLLFALFSVCFSDSVLSQTPYYPPLTGTTWQTVSPAEL
                     GWCENQLDSVYQYLAEKDTKAFIILKGGRIAAERYFGTFTRDSLWYWASAGKVLTAFM
                     VGQAQEDGLLDILQPTSTYLGTGWTSLPPDKEALITVRHQLTMSSGLDDGVPDDFCTL
                     PACLLYEADAGQRWAYHNAPYTLLTNVLNSATGQGLNVYFNAKIRNRIGMKGLWLPQG
                     YNQIYYSDARSMARFGLLILRHGVWASDTLMRDTAYFSAMVNTSQNLNPSYGYLWWLN
                     GKSSYKLPGLQFNFPGSIIQTAPADLFAGLGKNDQKLYVIPSLDMVVVRLGETAGGAN
                     PALSGFDNTLWAKLMEVFCNVSAQSEASVAPSFQMSPNPAGDLVRIQIPDPTQDFSIA
                     VRDAQGRLLLRQSNDPELRLEQFPSGVYFVTVRQGQRIQTQRLLHLRP"
     gene            2060..2569
                     /locus_tag="PK198_10545"
     CDS             2060..2569
                     /locus_tag="PK198_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39219.1"
                     /translation="MPFKFGLRGYIEPPQRHELSLEEFRRIFVLDFPESTTRPDLFQK
                     FMQYTDRFRSEITKDFIQWVGGSFTTRKLNPKDIDMVTFISPETFERKSELIDEEFRK
                     DCNLKYGIDAYFVVVYHEGHEKHSLYQGNVIYWDHQFSKTKKNRVGNRFSRGYVQITH
                     QKQSGHEQE"
     gene            2556..2783
                     /locus_tag="PK198_10550"
     CDS             2556..2783
                     /locus_tag="PK198_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39220.1"
                     /translation="MNKNKFAEVSERSARLSDLIEEIAQLDELIELHQSHHAHALEIQ
                     QYLDRRYEFVAELNVLLHPLHMKVVLEESPV"
     gene            2856..>3333
                     /locus_tag="PK198_10555"
     CDS             2856..>3333
                     /locus_tag="PK198_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39221.1"
                     /translation="MMYGTDIEALNRRIHEESAFVEQLSNETGRIIVGQERMMERLLI
                     GLLSRGHVLLEGLPGLAKTLAIKTLAQAIDARFSRIQFTPDLLPADIVGTMIYNPATH
                     EFSARKGPVFANFVLADEINRAPAKVQSALLEAMQERQVTIGHETFVLEEPFLVLAT"
     assembly_gap    3334..3360
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3361..3859
                     /locus_tag="PK198_10560"
     CDS             <3361..3859
                     /locus_tag="PK198_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF39222.1"
                     /translation="TYPLPEAQVDRFMLKVKVGYPGREEEREIIRRNVSGAIGEALRP
                     VVHPQAILRARESVRLVYMDEKIERYILDIVSATREPDKAGLARLKPLIQYGASPRAG
                     INLALAAKALAFLRRRGYVVPEDILELCPDVLRHRIGLSYEAEAENISTEDIIRDLLN
                     VVKMP"
     assembly_gap    4044..4069
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4070..4763)
                     /locus_tag="PK198_10565"
     CDS             complement(<4070..4763)
                     /locus_tag="PK198_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39223.1"
                     /translation="MSIEKMFLSVSVLLTMLLMYNFVPPCRDPNLFFWILIACIVPIV
                     FYCWAQRSKLSSLNKSAKKWFIAILLFLFTGVSIGYSFLYQTRVISDHINVEGYHEEI
                     FFIIPFNYPQNTQAKFPELEKVGWTKIAEGDIGYVQGWFADSEDNNYNYPLNALLVET
                     AFGVLLLIGFVSLLLLYILVYLKKTTNAKSLKNESGLSREVSELIAKDELIEACNMIL
                     QDPGIDVDSILHI"
     gene            complement(4766..>5117)
                     /locus_tag="PK198_10570"
     CDS             complement(4766..>5117)
                     /locus_tag="PK198_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39224.1"
                     /translation="GSSQSLGNEYLLEPVFLHTLEIKADAEQCYYIMVPIRDYEQGAE
                     EFKKLLKDYAASGKKPSKIPKYCDMNPVPYKITISKVIRYKFLCVRRAENEIREDVFT
                     FGEPLRDTIIFKFK"
     assembly_gap    5118..5139
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5140..5481)
                     /locus_tag="PK198_10575"
     CDS             complement(<5140..5481)
                     /locus_tag="PK198_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39225.1"
                     /translation="MKELISYALLFAMLYAVPVNAQTVINEISATPLRGDTIPTESEK
                     ELFHVTFHYKPKHRNENIIALNLLDANKKNLAVLEWHRNEFEYSIDSEVMTVRFKIDK
                     EWASKVAMVEPH"
     assembly_gap    5503..5523
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5716..>6808
                     /locus_tag="PK198_10580"
     CDS             5716..>6808
                     /locus_tag="PK198_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRF39226.1"
                     /translation="MRPFFIAAWLLFSLALSAQPTEKEALSTIRKVTVYLQGAQVSRT
                     AAVALPAGPTALKFTGLSPMLDPESIQVSALGNFTILSVQHQINYMKENEPDAEVKKL
                     QTEIQQLQIKTDREQAALSALEEEERFILANKTLGGEKGWTAEDLKAGADFYRSRLTE
                     LRLRKLDISLVVQKLNEDKKRFQNQINELNARQRNVATSEIVVNVSAPAAVSAEFALS
                     YLAPNASWTPSYDVRVQDITKPLALTYKAQVVQNTGENWSNVQLTLSTGNPSLNNTRP
                     ELQAWWLRFYEPVAYYGRRERMQTMDSPATMAKESVMSRDEDKAASGPAVEQVERTTT
                     QEFRIAQPYSIAGGTKPVTVVIAQEEVPAF"
     assembly_gap    6809..6818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6819..7321
                     /locus_tag="PK198_10585"
     CDS             <6819..7321
                     /locus_tag="PK198_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRF39227.1"
                     /translation="YCVPKLDKTAYLTAGIADWNSYRLLSGEALLFFEGSYVGKTYLN
                     TAETGDTLRLSLGRDESIVVERKREKEFADKQFLGGKITQTTGWNIELRNSKKQAVSI
                     VIEDQYPLSTSEEIEVKLEQSKGAVVNPATGQPLWKLNIAPGKTEKLNFRYSVRYPGK
                     RQLTLE"
     gene            7399..>7603
                     /locus_tag="PK198_10590"
     CDS             7399..>7603
                     /locus_tag="PK198_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF39228.1"
                     /translation="MRYFILPLFLAASMSLCAQQPAQYSLFMLNPMQWNPAYAGLDHS
                     LSISAGYRKQWTQLPGSPAGQYIA"
     assembly_gap    7604..7625
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7626..8352
                     /locus_tag="PK198_10595"
     CDS             <7626..8352
                     /locus_tag="PK198_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF39229.1"
                     /translation="LGLQVENDRLGAGRFSSAQLNYSFHRPLGNALLAVGFGAGITQR
                     SLDGSLIRTPDGVYEPGSGPNHNDPLLPVGLESGMTPTFHAGVYFQSERFEAGLGLRH
                     LTRPEVELPSFNFQLERSFFLQLGGHFDLGDKLSVHPALLVRSDMVETQTDVAALFRI
                     QQNLLAGIGFRGYSNDTGDAVIGMAGFQLSENLLLAYAYDATVSGLRNANNGSHEIML
                     QYNLNRPLVQARLPRIIYNPRTL"
     gene            complement(8357..>10575)
                     /locus_tag="PK198_10600"
     CDS             complement(8357..>10575)
                     /locus_tag="PK198_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF39230.1"
                     /translation="TAGNISAFDISPDGKKMAFVSRGELFVSDIKGKFVQQLPTRADG
                     RVLETYWLKDNKTILFNQTVNGYQNWFSIAADGSAPEKQHTSDLRNNRQLGLNSDRTQ
                     AVYLSGRDELRLMDLATFQSSLLLKDEFWAFQSDSPQFGPDDKHVLVSAYRNFERDLM
                     VVHTETKQVRNLTNTAVTESGGIWSPDGKAVYFTSNRTQPGYPYGLQRPDVFKMPLSL
                     MDEEPLRSDKFAEMFAEPDTSKAAKDKKSEKPVVAIADANLMEHIEATGPVFGAQFVV
                     HCSQKDSKTTLLLLSDHDEGRMALWKVLTEPFEKSKTEKIAGAEGFISSIVYVDGKYY
                     ALIGGNVHSLDMGGNKAEKIEINHTFRRNLRDEFQQMFYETWANMEENFYSEDFHGVN
                     WPAMRDRYAAWLPYVNTRADLRRLTNDLLGELNTSHFGFSSGGSEETVFYKNRTLSTG
                     IVFEESKPWQVQRVVANSVAARSQTAIRPGDVLTQVNGRDVDARANREMYFSRPSLDE
                     EMSLTFRRGTETVQVKLKPQSVFDVRDLMYDEWVADCQRRVDERSNKRIAYIHMKDMG
                     GDELERFLTEMVSEGAYRDGLILDLRWNTGGNVHDEVLRFLSQRPYLQWKYREGQLTQ
                     QSNFGPAVKPIVLLINEQSLSDAEMTAQGFKHLKLGKIVGTETYRWIIFTSGKGLVDG
                     SFYRLPSWGCYTLDGKNLEKTGVAPDIQIHNTFQDRLEGKDPQLDRAIEEVMKGMK"
     assembly_gap    10576..10587
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10588..>10957)
                     /locus_tag="PK198_10605"
     CDS             complement(<10588..>10957)
                     /locus_tag="PK198_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRF39231.1"
                     /translation="HRKGYKGAFNPDIKSYNPQTREFKVLTDYIGKDFNPVTDRQGNL
                     YFLSDEHNGEYNLYAFRQNKKTRLTNFKTSIRQLSAAADGSSLVFQRDYQIYRYDVKA
                     DKAAPVDIRVPRNLTLAQPQD"
BASE COUNT         2550 a         2747 c         2785 g         2548 t
ORIGIN      
        1 ttgcacctcc atgccctgca tttttttgga aataaaataa atcgtggtat cgccctcaat
       61 ggtagggctg atggccatga tgacctcttt gacctcgccg ccggctacgc gctgtatcag
      121 atcgtcaatg tgcagttgtt ccggcccgat gccctcaatg ggcgagatga caccgcccaa
      181 tacatgatac aatccccggt attgctgcgt gtcctcaatg gccatcacat cgcggatgtt
      241 ctccacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnncagggcc gttttgcgcc cgaggcccgg caggccggca aaagcctgca
      481 cggcttcttc taagagtttg gaagaaaaat tcatggacgc aaatgtagca atctttgcgt
      541 ttgtatcctt tgcccctgcc gaaccgtgac gaaatatacg cctgaaggaa actgctccaa
      601 ccggagttcg gggtcgttgg actgccggag caggagtcgc ccctgtgcgt cgcgaacggc
      661 tatgctgaaa tcttgcgtcg ggtcgggtat ctggatgcgc accaagtctc cggcggggtt
      721 gggcgacatt tggaaagagg gagcaaccga tgcttcgctc tgggcgctca cattgcaaaa
      781 gacctccatc agcttggccc acaaggtatt gtcaaaaccc gacaacgcag gattcgcccc
      841 tcctgccgtt tcgcccagtc gcaccaccac catatcgagc gagggaatga catagagttt
      901 ttggtcgttt ttgcctaagc cggcaaagag gtcggcgggg gctgtctgta tgatggaacc
      961 tggaaaattg aattgcaagc cgggcagttt atagctgctt ttgccgttga gccaccagag
     1021 atagccatac gacggattga ggttttgaga ggtgttgacc atggcgctga aataggcggt
     1081 gtcccgcatc agcgtgtcgg aggcccaaac tccgtgccgc aggatgagca ggccaaagcg
     1141 ggccatgctg cgcgcatcgc tgtaataaat ctgattgtag ccctggggca gccacaagcc
     1201 tttcatgccg atgcgattgc ggattttggc gttgaagtac acgttcaatc cctgcccggt
     1261 ggcgctgttg agcacattgg tgagcagcgt gtagggcgca ttgtgatagg cccagcgctg
     1321 tccggcatcg gcctcgtaga gcaggcaggc aggcaaggtg caaaaatcat ccggcacgcc
     1381 gtcatccagt ccgctgctca tggtcagttg gtggcgcacg gtgatgagcg cctctttatc
     1441 aggcggcagg cttgtccagc ccgtgccaag gtaggtggaa gtaggttgta gaatatccaa
     1501 cagcccgtct tcctgcgctt gtcccaccat aaacgcagtg agtacttttc ctgcggaggc
     1561 ccaataccaa aggctgtcgc gggtgaaggt gccgaagtag cgctccgcgg caatgcgccc
     1621 gcccttgagt atgataaagg ctttggtgtc tttttcagcg agatattgat aaacggaatc
     1681 cagctgattc tcgcaccagc ccagttcagc aggcgatacg gtttgccagg tagtgccggt
     1741 caggggtgga tagtaaggcg tttgagacag tacactatct gagaagcaga cagaaaacag
     1801 cgcaaacagg agaacaggca aggtattttt catatccggc ggattttgtg gccatatgac
     1861 tttccaatca agaaaaagtt tagccggagt tttgaaaacg cccgtattac caccctgatc
     1921 gcctattttt acgcccacaa ctgatggcca atgatgtacg gaaccgatat tgaagcgctc
     1981 aaccggctgg tgcgtaaaac cttcatagca ggcagagttg ctcctgaaga acagtatttc
     2041 aatcaaacaa tgaaatcaaa tgccctttaa atttggactg agaggatata tcgagcctcc
     2101 ccaaaggcat gagctgagct tagaggagtt tcgaaggata tttgtccttg actttccaga
     2161 aagcactacc agacctgatt tgtttcagaa gttcatgcaa tatacggata gattcaggtc
     2221 tgaaataacc aaggatttca ttcaatgggt tggcggaagt ttcactacgc gtaaactcaa
     2281 tcccaaagac atagatatgg tgactttcat ttcccctgaa acctttgagc ggaaatcaga
     2341 gttgattgat gaggaatttc gcaaagattg caacctaaaa tatggaattg acgcttactt
     2401 tgtcgtcgtt taccatgaag ggcatgaaaa gcactcctta taccagggaa acgtcatata
     2461 ttgggatcat caattctcaa aaacaaaaaa gaacagggtc gggaatagat tttcacgagg
     2521 ttatgttcag attactcatc agaaacagtc agggcatgaa caagaataaa ttcgcagaag
     2581 tgtcggaaag aagcgccaga ctcagcgatt tgattgagga aatcgcccaa ttggatgaac
     2641 ttattgaatt acatcaatcg caccatgctc atgcgcttga gattcaacag tacttagacc
     2701 ggcgatatga atttgtagct gagctaaatg tgctcctgca cccgttacac atgaaagtgg
     2761 tgctggagga aagccctgtt taatctctat tcctactcca tcttcatgtt taccacgatc
     2821 agatcgccta tttttacgcc cacaactgat ggccaatgat gtacggaacc gacattgaag
     2881 cgctcaaccg gcgcatacac gaggaaagcg cttttgtaga acaactgagc aacgaaaccg
     2941 gccgcatcat tgtggggcag gaacggatga tggagcgatt gctcatcggg ctgctcagcc
     3001 gggggcatgt gttgctggaa gggctgccgg gattggccaa aacattggcc atcaaaacat
     3061 tggcacaagc cattgacgcc cggttcagcc gcatacaatt cacgcccgac ctgctgcctg
     3121 ccgacatcgt gggcaccatg atttacaacc ccgccacgca cgagttcagc gcccgcaaag
     3181 gccccgtatt cgccaatttt gtgctcgccg acgagatcaa ccgcgccccg gccaaggtgc
     3241 aaagcgcgct gctggaggcc atgcaggagc ggcaggtgac gataggccac gagacttttg
     3301 tgctggagga gccttttctg gtgttggcca cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 cacctacccg ctgccggaag cacaggtgga ccgcttcatg ctgaaggtga aggtaggcta
     3421 tccgggccgc gaggaggagc gcgaaatcat acgccgaaat gtgtcgggcg ccataggcga
     3481 ggccctgcgg ccggtggtgc atccgcaggc cattttgcgg gcgcgcgaat cggtgcgatt
     3541 ggtgtacatg gatgaaaaaa tagaacgcta catattggac attgtgtcgg ctacccgcga
     3601 gccggataag gccggacttg cccggctcaa acccctgatt caatatggcg cctcgccccg
     3661 cgcgggcatc aatctggcgc tggcagccaa agccctggcc tttttgcgca ggcgcggcta
     3721 tgtggtgccc gaagacattc tcgaattgtg ccccgacgtg ttgcgccacc gcatcggtct
     3781 gagttatgag gccgaggccg aaaacatcag cacggaagac atcatccggg acttgctgaa
     3841 cgtggtgaag atgccgtgag gggggatggt cttgttgagg gaaaaataga ttttcaggtt
     3901 ctcagactga aggatttttc aaggtgctaa gtggtgtttt aactcttgat ttttaataag
     3961 gccattcttt gtttacttct atgatgagaa taatcgtcta aagtaatcaa acttcccaga
     4021 tatttttttt cagtatcacc gatnnnnnnn nnnnnnnnnn nnnnnnnnnc tatgtgtaaa
     4081 atcgaatcta cgtcaatacc gggatcctgt agaatcatat tgcaagcctc aataagttca
     4141 tccttagcga ttaactcgct cacctccctg ctcagaccgc tttcgttttt caaagactta
     4201 gcattggttg tctttttcaa atacaccaat atgtatagaa gcaataatga aacaaaaccg
     4261 atcagcagga gtacgccaaa agcagtttca acgagtaagg cattaagcgg atagttatag
     4321 ttattatcct cgctatcggc aaaccagcct tggacgtagc ctatatcacc ttcggcaatt
     4381 tttgtccacc ccactttttc caactcaggg aatttggctt gtgtgttttg aggataattg
     4441 aagggaataa taaaaaagat ttcctcatga tatccttcta catttatatg atcagatatg
     4501 acacgcgttt gatacaagaa agaatagcca atagataccc ccgtgaataa aaaaagcaaa
     4561 attgctataa accacttttt tgcggactta ttgagggatg ataatttact cctttgcgcc
     4621 caacagtaaa atactatcgg gacgatacaa gctataagaa tccagaaaaa tagattcggg
     4681 tctctacacg gaggaacaaa gttgtacatc aacaacattg taagtaatac cgatacggat
     4741 agaaacattt tttctatgct catattcatt taaatttaaa tattatcgta tctctcaagg
     4801 gttcgccaaa ggtgaaaacg tcttctctta tctcattttc cgcacgcctc acgcataaaa
     4861 atttatacct aatgacttta gatattgtta ttttataggg aaccggattc atgtcacagt
     4921 atttggggat tttactgggt tttttaccgg aagcggcata gtcctttaag agttttttga
     4981 actcctccgc tccctgttca taatccctga taggaaccat aatatagtaa cactgctctg
     5041 catcagcctt gatttcaagg gtatgcaaga atacgggttc aagtaaatat tcgtttccca
     5101 aggattgtga gctgcccnnn nnnnnnnnnn nnnnnnnnna tgaggctcga ccatagctac
     5161 ttttgatgcc cattctttat ctattttaaa tctgacagtc ataacttctg agtcaatact
     5221 atattcaaac tcgttgcgat gccattccaa tactgctagg ttcttcttgt ttgcatccaa
     5281 gaggttgagt gcaataatgt tctcattacg atgcttaggt ttgtaatgaa aagtaacgtg
     5341 aaataactct ttctcgcttt ccgtgggaat agtatctccc ctaagtgggg ttgccgatat
     5401 ctcattgata acggtttgag cgtttacagg caccgcgtaa agcatggcga acaacaaagc
     5461 gtatgagata agctctttca tgtgtaattc agtatttcgt ttnnnnnnnn nnnnnnnnnn
     5521 nnnaaactct aaaaaacttg ttaatataac aaataaaaag ccttgtctaa gtatatcagt
     5581 ataaatatat ttttttttac aatatttatt tttatgcctg ttaatgagaa cgctcttttt
     5641 aaagagcaat aatttttggg gatatcttta tgcaatttcc cccaccactg catctttcta
     5701 taaaaaaaca tgaacatgag accgtttttc atcgcagcat ggctgctctt ttctttggcg
     5761 ctgagcgcac aacccactga aaaagaagca ctttccacca ttcgcaaagt gaccgtttac
     5821 ctgcaaggcg cccaagtatc ccgaacggca gcggtggcat tgcccgccgg cccgacggca
     5881 ttaaaattca ccggcctctc tccgatgctc gatcccgaaa gcattcaggt gagcgctttg
     5941 gggaatttta ccatcctttc ggtgcaacac cagatcaact acatgaaaga aaacgaaccg
     6001 gatgcggagg tgaaaaaatt gcagacggaa attcagcaac ttcaaatcaa aaccgaccgc
     6061 gaacaggccg cgctctctgc attggaggaa gaggaacggt ttattttggc caacaaaacc
     6121 ctcggcggcg aaaaaggctg gacggctgaa gacctcaagg ccggcgctga tttttacaga
     6181 agccgcctca ccgaactgcg cctgcgcaaa ttggacatca gcttagtcgt tcaaaaactg
     6241 aacgaagaca aaaagcgctt ccaaaaccag atcaacgaac tcaatgcccg gcagcgcaat
     6301 gtggccacca gcgaaattgt ggtcaacgtc tccgctccgg cggcggtatc tgccgagttc
     6361 gcgctgagct atttagcgcc caatgcaagc tggacgccca gctacgacgt tcgtgttcag
     6421 gatattacca aacctctggc gctgacttac aaggcgcagg tcgtgcaaaa taccggcgaa
     6481 aactggtcca atgtgcagct cacgctctct accggcaatc cttcgctgaa caatacccgc
     6541 cccgaactgc aagcctggtg gctgcgcttc tacgagccgg tggcctacta cggtcgccgc
     6601 gagcgcatgc aaacgatgga cagcccggcg acgatggcaa aggaaagcgt catgtcaaga
     6661 gatgaggaca aggccgcgtc cggcccggcg gtggagcagg tagaacgcac caccacacag
     6721 gagtttcgca ttgcccagcc gtacagcatt gccggcggta ccaagccggt gacggtggtg
     6781 attgcacagg aagaagtgcc tgctttttnn nnnnnnnnac tactgcgtgc cgaaattgga
     6841 caaaaccgct tacctcactg cgggcatcgc cgactggaac agctaccgcc tgctgagtgg
     6901 agaggcactg ctgttttttg agggatcgta tgtgggcaaa acctacctga acacagccga
     6961 aaccggcgat accctgcgcc tgtcgctggg ccgcgatgag agcattgtgg tagaacgcaa
     7021 gcgcgaaaag gaatttgcag acaagcagtt tctgggaggc aaaatcaccc aaaccacggg
     7081 ctggaacatc gagttgcgca acagcaaaaa gcaggccgtc agcatcgtga tcgaagacca
     7141 gtacccgctt tcgacgagcg aagaaataga ggtgaaattg gagcaatcca aaggcgccgt
     7201 cgtcaatccg gccaccggcc aacccctgtg gaaactcaac atagcgccgg gcaaaaccga
     7261 aaaactcaac ttccgctatt cggtgaggta tcccggcaaa cggcagttga cgctggaatg
     7321 atttcccgcg gcggcctctc gtggaaaact cttgaggccg ccgcgtgttt cagaagagta
     7381 ccaacatcag gccggccaat gcgatatttt attcttccgt tgtttcttgc cgcaagcatg
     7441 tcgctctgtg cgcaacaacc cgcgcagtat tccctgttca tgctcaatcc gatgcaatgg
     7501 aacccggctt acgcgggcct cgatcattcg ctctccatca gcgccggtta tcgcaagcaa
     7561 tggacgcaac tgccgggaag ccctgccggg caatacatcg ccannnnnnn nnnnnnnnnn
     7621 nnnnnactgg gcctgcaagt cgaaaatgat cgcctcggcg ccgggcgatt cagttcggcc
     7681 cagctcaact acagcttcca tcgcccgctc ggaaatgccc tgctcgccgt tggcttcggc
     7741 gccggcatca cgcagcgcag cttagacggc tctctcattc gcacccccga cggcgtgtac
     7801 gaacccggct ccggccccaa tcacaacgac ccgctgctgc cggtcggcct cgaatcgggc
     7861 atgacgccta cttttcacgc cggcgtttat ttccagtctg agcgcttcga ggcaggtttg
     7921 gggctgcgcc atcttacccg cccggaagta gaactgcctt ctttcaattt tcagttggaa
     7981 cgcagctttt tcctccaact cggcggccat ttcgatctcg gcgacaagct atccgtgcat
     8041 ccggccctgc tcgtgcgcag cgatatggtg gaaacccaaa ccgacgtggc ggccctgttt
     8101 cgcattcaac aaaacctgct ggccggcatc ggattcagag gatacagcaa tgacaccggc
     8161 gatgcggtca tcggtatggc gggcttccaa ctgagtgaaa acctgctgct ggcctacgcc
     8221 tacgatgcca ctgtttccgg cctgcggaac gccaacaacg gctcgcacga aatcatgctt
     8281 caatacaacc tcaaccggcc cttggtgcaa gcccgactac cccgcattat ttataatccg
     8341 aggactttgt gaaatcctac ttcatcccct tcatcacctc ctcaatggcg cgatccaatt
     8401 gcgggtcttt gccttcgagc ctatcctgga aggtgttgtg aatctgaata tcgggagcaa
     8461 cgcctgtttt ttccaagttc ttgccgtcca aagtgtagca accccaggaa ggcaggcggt
     8521 aaaacgagcc gtccaccagc cccttgccgg aagtgaagat gatccagcgg taggtttccg
     8581 tgccgacaat cttgcccaat ttcaggtgtt tgaaaccctg cgccgtcatt tctgcatcgc
     8641 tgaggctttg ctcgttgatg agcagcacga tgggcttcac cgccggcccg aaattgctct
     8701 gctgcgtgag ttggccctcg cggtatttcc attgcagata aggccgctgc gagaggaaac
     8761 gcagcacctc atcgtgtacg ttgccgccgg tgttccagcg caggtcgaga atgagaccgt
     8821 cgcggtaggc gccctccgaa accatctcgg tgaggaagcg ctccaattca tcgccgccca
     8881 tgtctttcat gtgaatgtag gcaatgcgtt tgttgctacg ctcgtccacg cggcgctggc
     8941 aatcggccac ccactcgtcg tacatcagat cgcggacgtc aaaaaccgat tgcggtttca
     9001 gtttcacctg caccgtttcc gtgccgcggc ggaacgtgag gctcatttct tcatccaaag
     9061 acgggcgcga gaagtacatt tcccggttgg ccctggcgtc cacatccctg ccgttcactt
     9121 gcgtgaggac atcgccgggc cggatggcgg tttgtgagcg ggcggcgacg ctgttggcca
     9181 ctacgcgttg cacctgccag ggcttggatt cctcaaaaac gatgcccgta gagagcgtgc
     9241 ggtttttgta aaaaacagtt tcttcactgc cgccggaact gaagccgaag tgcgacgtat
     9301 tgagttcgcc gagcaaatcg ttggtgaggc ggcgcaggtc ggcgcgggtg ttcacatatg
     9361 gcagccaggc ggcgtagcgg tcgcgcatgg cgggccagtt cacgccgtgg aagtcttcgg
     9421 agtagaagtt ttcttccatg ttggcccagg tttcgtaaaa catctgctgg aactcgtcgc
     9481 ggagattgcg gcggaaggtg tggttgatct cgattttttc agctttgttg ccgcccatgt
     9541 ccaagctgtg tacattgccg ccgatgaggg cgtaatattt gccgtccaca tacacgatgc
     9601 tgctgatgaa gccctcagcg ccggcaattt tttccgtttt ggatttttca aatggctccg
     9661 tcagcacctt ccacagcgcc atgcgtcctt catcgtggtc ggacagcagg agcagggtgg
     9721 ttttactgtc cttttgcgag cagtgtacga caaattgggc gccaaagacc ggcccggtgg
     9781 cctcaatgtg ctccatcaaa ttggcgtcgg caatggcaac gaccggcttt tcagacttct
     9841 tgtctttggc ggctttgctg gtatccggct cggcaaacat ctcggcgaac ttgtcggaac
     9901 gcagcggctc ctcgtccatt aaactcaagg gcattttgaa cacatccggg cgctgaagtc
     9961 cgtaaggata gcctggctgg gtgcgattgg aggtaaaata aaccgccttg ccgtcgggcg
    10021 accagatgcc gccggattcc gtcactgcgg tgttggtgag gttgcgcacc tgtttggttt
    10081 ctgtatggac gaccatcaga tcgcgctcaa aattgcgata agcggaaacg aggacatgct
    10141 tgtcgtcggg gccaaactga ggactgtcgc tttggaatgc ccaaaattcg tctttcagca
    10201 acagcgaact ttggaatgtg gccaaatcca tcagccgcag ttcgtctctg ccactcagat
    10261 acacggcctg ggttcggtcg ctgttgaggc cgagctggcg gttgttgcgc aggtcgctgg
    10321 tgtgttgctt ttcgggagcg gaaccatcgg cggcgatgct gaaccagttt tggtagccgt
    10381 tcaccgtctg gttgaagagg atggttttgt tgtctttcag ccagtacgtt tccagcacgc
    10441 ggccatcggc gcgggtgggc aattgttgta caaatttgcc cttgatgtcg gacacaaaga
    10501 gttcgccgcg agacacgaag gccatttttt tcccgtcggg cgagatgtcg aacgccgaga
    10561 tgttgccggc agtttnnnnn nnnnnnnaat cctgcggctg tgccagcgtg aggttgcggg
    10621 gcacgcgaat gtccaccggc gcggccttat cggcttttac atcgtagcga taaatctggt
    10681 agtcgcgctg aaacacgagg ctcgacccgt cggcggcggc gctgagttgg cggatggagg
    10741 ttttgaaatt ggtgagtcgc gtctttttgt tttgccggaa ggcgtagaga ttgtactcgc
    10801 cgttgtgctc atcggagagg aagtagaggt tgccctgccg gtcggtgacg gggttgaaat
    10861 ccttgccgat gtagtcggtc agcaccttga actcgcgggt ttgagggttg taagacttga
    10921 tatcgggatt gaaagcgcct ttgtagcctt tgcgatg
//
LOCUS       DAORLF010000111        10943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3217_length_10943_cov_2.137583, whole genome shotgun sequence.
ACCESSION   DAORLF010000111 DAORLF010000000
VERSION     DAORLF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3217_length_10943_cov_2.137583"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>894)
                     /locus_tag="PK198_10610"
     CDS             complement(<1..>894)
                     /locus_tag="PK198_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRF39232.1"
                     /translation="LRSLNEDHPSIQEINERITALRGVVLSTLRNVRGELQQKANTIR
                     QKNKQLQGSLRSMPGIERELIEITRQKNIKENLYLYLLQKREEAAISMAVAASNSRVL
                     DAAQPATAPIEPKRQIAYALAILLGLGIPLGIAVLADLLNDTVQTADDIKNTTQAPLA
                     GTIGHSRSGQHLVVNKSTRSAVAEMFRLLRANLQFLHPGNDSQVWLVTSGSSGEGKSF
                     ISLNLGMTLAIGGKRVAILGMDLRKPKLLKYLGIEDDEGGRRLRGITHYLIGEAELSQ
                     ILHPTGLHDKLFFAPSGPIPP"
     assembly_gap    895..912
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<913..2037)
                     /locus_tag="PK198_10615"
     CDS             complement(<913..2037)
                     /locus_tag="PK198_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39233.1"
                     /translation="MTNTPDPFLDADKPIDLRLLLYKYLLRFWWLYILGILLAGAGAW
                     LYLRYTTPQFLVRSTMLIKTPESSTGDVSQEFILGELGLAPTGGNVENEMQILKSRSL
                     MREVVDRLKLHIIYEAEGRIKRGEQYLRSPVLLSYAALSDSLRGPAFEIDPQPDDSTQ
                     FILRRGESWSKTAAFGDTLRTDWGDFTFIRNPEAPKWSSEGQTIIRFSSPDAEAQKYS
                     ARIQMKKVGEWSSVLELTLKDPVSQKAADILNTLAIVYNEAAIQDKNRVASSTYDFIN
                     ERLKFLTAELSESEGELESFKTRKVIPGTIMDNVSSALDEFKINDAELSRLQLQQDVV
                     GNLEAILNAANGPGDLLPANFVVENADLNSQTERYNELILE"
     gene            complement(2101..3306)
                     /locus_tag="PK198_10620"
     CDS             complement(2101..3306)
                     /locus_tag="PK198_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39234.1"
                     /translation="MNKSAIQIWIEAGEGLQTEFKTSFGPEVIESLAAFTNTSGGRVF
                     IGVSDSGAVSGVTTQPESAQQWVNEIKTKTSPSIIPDIEWYEVDGKNIVVLSIQEHPV
                     KPVAVRGKYYKRVGNANHLISTSEAVNMHLRSFNTSWDYQISLQHNLDDISLDKVQKA
                     IDQANAVHKNITEDPITFLMKSDLLRNGELTYAAYLLFHKNEAVVANIELGRFQDDIT
                     IKDSDRSRSDLLTQTEEVMAFVKKHIHKEIVISGQIQHQEKWQYPPEALREIILNMII
                     HRDYRSSADSVVKIYDHKIEFYNPGKLPESISIQDLLSNHYTSSPRNKLIADFCKSIG
                     IIEKYGSGIRRILHATQTHGLPQPLFQQITDGFMVTIFDAQYSATQNDISKGGTGFFK
                     NIKNFFASF"
     gene            complement(3314..4081)
                     /locus_tag="PK198_10625"
     CDS             complement(3314..4081)
                     /locus_tag="PK198_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRF39235.1"
                     /translation="MSLKIASALLLGVLALSSCVKHRELVNFNQGPEFPAQDAIGALP
                     VLRIQPDDALYITVHTLEPADAAPFNISPGQSANAMAGANAANYLVDDQGAIEMPVIG
                     SVAVGGLSVAEARDTIRNRLLPYLKSPIVNIRFTQFRFTVLGEVKNPTTFTLAEERIS
                     ILEALGMAGDLTNYANRNNILVIREENGQRSFGRIDVQARDVFRSPYFYLRPNDVIYI
                     EPTKNKTGAVTDQFTKALPWIGAGTAFLNLVLILTRR"
     gene            complement(4078..4833)
                     /locus_tag="PK198_10630"
     CDS             complement(4078..4833)
                     /locus_tag="PK198_10630"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39236.1"
                     /translation="MFQLFSKKNIPSDFSFLHTDLHSHLIPGIDDGAKTLEDSLQLIA
                     RLSKLGYRHIITTPHVMADLYPNTPDDILSGLEKVRAAVANKKIDIEIDAAAEYFMDE
                     HFAPLVEHDQLLTLPGNRVLVEMSTLNSPPNLFNDLFRLQTKGYQPVLAHPERYVFLK
                     EDFRQYTRLKDYGCEFQLNLLSITGYYGKPVRDIALKLLKNKMVDLLGTDMHHERHAA
                     SLEAMAADKSMMKILADYEFANAALSEKVEEIL"
     assembly_gap    4983..5107
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5165..5341)
                     /locus_tag="PK198_10635"
     CDS             complement(5165..5341)
                     /locus_tag="PK198_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39237.1"
                     /translation="MEIMPADKILIINGHPDAESYNFALAEAYKKGALASVRFAPLPS
                     APFGFPKTATGPIG"
     gene            complement(5322..5693)
                     /locus_tag="PK198_10640"
     CDS             complement(5322..5693)
                     /locus_tag="PK198_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39238.1"
                     /translation="MAAAIFFCYRPLLSGLENIWADYNKGLVFLGLGVSFSTLQDTTK
                     TQNKLSKRIWENPRYARIFLIFLSFLIVVFIGMGLVGMFLTDIQALQELSFGFLIFSI
                     GMLGMLKAAVEMAENHGNHAR"
     gene            complement(5800..6339)
                     /locus_tag="PK198_10645"
     CDS             complement(5800..6339)
                     /locus_tag="PK198_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF39239.1"
                     /translation="MKYLLLQQESERLLFRPQHRDDFAEWLPAMEHPDTERFLGMTGI
                     STAEERCETWFQRVENRYQNDLGGTNVLVDKASGKIVGQCGLLVQEVDGVQELEIGYT
                     IISAYWNRGYATEAARKCKEFAFENNFADSIISIIHVENIRSEMVARKNGMQLDKTTT
                     FKGFPVNIFRIRRADFEAS"
     gene            complement(6336..7124)
                     /locus_tag="PK198_10650"
     CDS             complement(6336..7124)
                     /locus_tag="PK198_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRF39240.1"
                     /translation="MFYMPFIVLAALILLSGIRVAQEYQRSIIFKLGRYTGIKGPGIY
                     WLIPLIERQQKVDIRTRTVDLEQQETITRDSVTIKVNAVLWFKIIDPESAIIKVANYN
                     NAVYQFSVSALRNIIGQHTLDEVLRDREQINNTLKKIVDESTEPWGVKIEMVEMKDVE
                     IPEGMQRAMAREAEAIREKRARIVKAEAELDASIKLTQGAKEMEASPIALELRRMQML
                     SEIGIDNNTTTIVLVPSDFAQAARSFTDWTNQRLTQPSHKPDTQ"
     gene            complement(7136..8188)
                     /locus_tag="PK198_10655"
     CDS             complement(7136..8188)
                     /locus_tag="PK198_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRF39241.1"
                     /translation="MKILVTGAAGFIGFHLINRLIARGDEVVGLDVLNDYYDIQLKYN
                     RLAYAGIQKDEIAYGKLVQSALHPNYRFVQTGLEDSAALNELFAAERFDAACNLAAQA
                     GVRYSLVNPRAYIDSNIVGFANLLEACRHNEVKHLAYASSSSVYGLNEQMPFSTQDNV
                     DHPVSLYAASKKSNELMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTKAILAG
                     EPIQVFNHADMIRDFTYIDDIVEGVVRVLDHPPAGNSGWSGLQPDPSSSRAPYKVYNI
                     GNQNPVRLMDFVQAIEAALGRNAVIDFQPIQPGDVPATYADVSDLVKEMNYKPQTSVQ
                     DGIQAFIQWYRQYYHA"
     gene            complement(8479..9441)
                     /locus_tag="PK198_10660"
     CDS             complement(8479..9441)
                     /locus_tag="PK198_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF39242.1"
                     /translation="MNFALIGAAGFVAPRHMRAIKDSGHQLLAAYDPNDSVGVIDSHF
                     PQADFFTEFERFDRHLEKLKRSGQHIDYVVVCSPNYLHDAHIRFGLRLGADVICEKPL
                     VLNPWNVEALQDMERETGRKVYTILQLRLHPAIIELRERVQSSPTLLQHEVDLSYITS
                     RGKWYYTSWKGDLNKSGGIATNIGIHFYDMLQWVFGPVRQNVVHVHTHDRAAGFLELE
                     RARVRWFLSINADTLPAEVQAKGGRTFRSITVDGQELEFSEGFTDLHTLSYQDILSGK
                     GFGLEEAGIAIRTVHNIRKAVPVGLEGDFHPLAALPLSEHPFGR"
     gene            complement(9592..10443)
                     /locus_tag="PK198_10665"
     CDS             complement(9592..10443)
                     /locus_tag="PK198_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRF39243.1"
                     /translation="MTVNNAQALFDAIGNDVEITLEDGKYDLSTVNGGSEFADIFDFS
                     EYQMEDRTIRIKDVKNLHFVAKNKGKVEWVIPYAFIPVFKIEGGFNISFSGITMGHDV
                     QKGLCEGAVMTVLNANYLHIENCDLYGSGTMGIEAEGVSFLTMQRSVIRECTYGILSL
                     MSSNSFVFDDCTFKDNTGDNMVKLQFAYGLKFNNCQFINNQTPKEYGPYEFFRFQDDN
                     QTVSLNNCTFTNCTSDYFATYAEAIEEKNVNRKGLKTNKGLWRQKEAHITQWPEGETV
                     EDAAEEK"
     gene            complement(10430..>10943)
                     /locus_tag="PK198_10670"
     CDS             complement(10430..>10943)
                     /locus_tag="PK198_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39244.1"
                     /translation="VLLDNTGKVLVPFEYNYLLSSRNYLIASKGEGKTGIISTTNQVL
                     LPLEYDHVEVVAPGRFQVDKNGKRGLLDDKGKTLLPIEFDMPTHGEGGEYIEAMERPY
                     TAILFQKGGKGHLYNLNGKKISEKQWTFCGVPDAFGLTYATDINGRESYIGPDGAIYS
                     KDAPLKSDGQ"
BASE COUNT         2420 a         2618 c         2877 g         2885 t
ORIGIN      
        1 tgggcggaat ggggccggaa ggcgcgaaaa acagcttgtc gtgcagaccg gtaggatgca
       61 atatttgaga cagctcggct tcgccaatga ggtagtgggt gatgcctcgc aggcggcggc
      121 ccccctcgtc gtcttctatg ccgaggtatt tgagcagttt gggtttgcgc aagtccatgc
      181 cgagaatggc tacccgtttg ccgccaatag ccagggtcat gcccagattg agactgataa
      241 aactcttgcc ttcgccgctg gagccggaag tgacgagcca aacctgactg tcgttgccgg
      301 ggtgtaaaaa ttgcaggttg gcccgcaaca ggcggaacat ttcagctacg gcagatcggg
      361 ttgatttgtt gacgaccaag tgctgaccgc tgcggctgtg gccgatggtt ccggcaaggg
      421 gcgcttgggt cgtatttttg atgtcgtcgg cggtttgtac ggtgtcgttg agcaagtctg
      481 ccaacacggc aatgcccagc ggaatcccca atcccaataa aatggccaag gcgtaagcaa
      541 tctggcgctt aggttcaatg ggcgcggtag caggctgtgc ggcatccagc acgcggctgt
      601 tggaggcggc cacggccata gaaatggcgg cttcttcgcg tttttggagc aggtataagt
      661 agagattttc cttgatgttt ttttggcgcg tgatttcaat cagttcgcgc tctatacctg
      721 gcatggagcg cagggaaccc tgcagctgtt tgtttttttg acgaatggtg ttggcttttt
      781 gttgcagttc gccgcgcaca ttgcgcaggg tgctgagcac cacgccccgg agcgccgtga
      841 tgcgttcgtt gatttcctga atggaggggt ggtcttcgtt gaggctgcgc aggcnnnnnn
      901 nnnnnnnnnn nnttcgagga tgagttcgtt gtagcgctcg gtttggctgt tgaggtcggc
      961 attttctacc acaaaattgg cgggcagcaa gtcgccgggg ccgttggctg cgttgagtat
     1021 agcctccaga ttgccgacga catcctgctg aagttggagg cgggaaagtt cggcgtcgtt
     1081 gattttaaat tcatccaatg ccgagcttac gttgtccatg atggtgcctg gaatgacctt
     1141 gcgggttttg aaggattcca gttcgccttc tgattcagag agttcggcgg tgagaaattt
     1201 gaggcgctcg ttgatgaagt cataagtgct ggaggcgacg cggtttttat cctgaatggc
     1261 ggcttcatta tagacgatgg ccagggtatt gagaatatcg gcggcttttt gcgaaacggg
     1321 gtctttaagc gtcaattcca gcacgctgct ccactcgcct acttttttca tttgtattct
     1381 ggcggaatac ttttgagcct cggcatccgg agaactgaaa cgaatgatgg tttggccttc
     1441 gctgctccat ttgggcgcct ctggattgcg gatgaacgtg aagtcgcccc agtcggtgcg
     1501 caaagtatcc ccgaaagcgg cggttttaga ccagctttct ccccggcgca ggatgaattg
     1561 ggtgctgtca tcgggctgcg ggtcaatctc gaatgccggg ccgcgcaaac tgtcgctgag
     1621 cgctgcataa ctcagcaaaa ccgggctgcg gaggtattgc tcgccgcgct tgatgcgccc
     1681 ttccgcctca taaataatat gcagtttcag gcgatccacc acctcccgca tgaggctgcg
     1741 cgatttgagg atttgcattt cattttccac attgccgccg gtaggggcca ggcccagttc
     1801 gccgaggatg aattcctgcg aaacatcgcc ggtgctgctt tcgggcgttt tgatgagcat
     1861 ggtactcctc accaaaaact gcggcgtggt gtagcgcagg tagagccatg cgccggcgcc
     1921 ggccaataaa atgcccagta tgtacagcca ccagaaacgg agcaggtatt tgtacaacag
     1981 caagcggagg tcaatcggct tgtcggcgtc caaaaaagga tcgggggtgt tggtcataga
     2041 agagcattat gaatttcgga ttcctgattt cggattccgg aattttgaat ccggaatttc
     2101 tcagaacgat gcgaagaaat tttttatgtt tttgaaaaaa ccggtaccgc ctttggaaat
     2161 gtcgttttgg gtggcggaat attgcgcgtc aaaaatagtg accataaaac catcggtaat
     2221 ttgctgaaaa agcggctgtg gtaatccgtg cgtttgtgtt gcatgtaaaa ttcggcgaat
     2281 gccggagccg tatttctcaa taataccaat ggatttgcaa aagtctgcaa tgagtttatt
     2341 tctggggctg gaggtgtaat gattgcttaa taagtcttga atactaatgc tttcggggag
     2401 tttccccgga ttgtaaaatt caattttatg atcataaatt tttacaacgg agtctgccga
     2461 ggagcgataa tccctgtgaa taatcatgtt caggataatt tcccgaagag cttccggtgg
     2521 atattgccat ttttcctgat gttgaatctg accggaaatg acgatttctt tatggatgtg
     2581 tttttttaca aatgccatca cctcttccgt ctgagtcagc agatcggatc gacttctgtc
     2641 actgtcctta attgtaatat catcctgaaa ccggcctaat tcaatattgg cgacaaccgc
     2701 ctcattttta tgaaagagta aatacgcggc ataagtcagc tcaccattgc gtagtaaatc
     2761 gcttttcatt aaaaaagtga tggggtcttc cgtaatgttt ttatgtactg cattggcttg
     2821 gtcaatggct ttttgcactt tatccaacga aatgtcgtcg aggttgtgtt gcaggctgat
     2881 ttggtaatcc caactggtat taaaagaccg caagtgcata ttgaccgcct ccgaagtgct
     2941 gatcaggtga ttggcattgc ctactctttt gtaatacttg ccgcgcaccg ccacgggttt
     3001 taccggatgc tcctgaatgc tgagtacaac gatatttttt ccatccactt cgtaccattc
     3061 tatgtcgggt ataatggacg gggatgtttt tgtttttatt tcattgaccc attgttgggc
     3121 agattccggc tgggtagtta ctccgctgac ggcgcctgag tctgaaacgc cgataaaaac
     3181 ccttccgccg gaggtattgg taaacgccgc caaactctca atgacttccg ggccaaagga
     3241 ggttttaaac tcagtttgca ggccctctcc ggcttctatc cagatttgta tggcagattt
     3301 attcataagt atatcaacgc cgggtcaaaa tcaaaaccaa attcaaaaac gccgtccctg
     3361 ctccaatcca gggcaaggct ttggtaaatt gatcggtcac agcgccggtt ttatttttcg
     3421 taggttctat ataaataacg tcattggggc gcagataaaa atagggcgag cgaaaaacat
     3481 ctctggcctg cacatcaatt ctgccgaaag aacgctgtcc gttttcctcc cgaataacga
     3541 gaatattgtt gcgattggca tagttggtca ggtcgccggc catacccagc gcctccaaaa
     3601 tgctgattct ctcttctgca agggtgaagg tggtcggatt ttttacctct cccaacaccg
     3661 taaagcgaaa ttgcgtgaag cgaatattga caatgggcga tttgaggtag gggaggagcc
     3721 tgtttctgat ggtgtcgcgg gcttcggcta cgctcagccc tccgaccgcg accgagccta
     3781 tgaccggcat ttcgatggcg ccctgatcgt ccaccaaata attggcggca ttggcgccgg
     3841 ccatagcgtt ggccgattgc cccggcgaga tgttgaacgg cgccgcatcg gcaggctcca
     3901 gcgtgtgtac ggtgatgtag agcgcgtcat cgggctgaat gcgcagcacg ggtaaagcgc
     3961 cgatggcatc ttgcgccgga aattccgggc cttgattgaa attgaccaat tccctgtgct
     4021 tcacacaaga cgacagggcg agtacgccca acaacagggc ggaggcaatt tttaaactca
     4081 tagtatttct tctacttttt cagaaagggc ggcattggca aactcatagt cggccaatat
     4141 tttcatcatg cttttatcgg cggccatagc ttccagcgag gcggcgtggc gctcgtggtg
     4201 catatcggtg ccgagcagat caaccatctt gtttttcagc agtttcaggg cgatgtcgcg
     4261 cacgggcttg ccgtaatatc cggtaatgga aagcaggttg agttgaaatt cgcagccgta
     4321 atccttgagc cgcgtgtatt gccggaagtc ttctttgaga aaaacgtagc gttcgggatg
     4381 cgccagaacc ggctgatagc ctttggtttg caggcgaaac aggtcgttga acaaattggg
     4441 cggcgagttg agggttgaca tttccaccag cacccggttg ccgggcaagg tgaggagttg
     4501 gtcgtgctcc acaagggggg caaaatgctc gtccatgaag tattcggcgg cggcgtctat
     4561 ttcaatatct atttttttgt tggccacagc ggcgcgtact ttttccaatc cgctcaaaat
     4621 atcatccggc gtattgggat acaaatcggc cataacgtgc ggcgtggtga tgatgtgccg
     4681 gtagccaagc ttcgataagc gcgcgatcaa ttgcagcgaa tcctctaagg tctttgcgcc
     4741 gtcgtcaatg ccgggaatga gatggctgtg caggtcggtg tgcagaaacg agaagtcgga
     4801 agggatgttt ttcttgctaa aaagctgaaa cattggatgg tagatttaaa atttcgggtt
     4861 ttatccaccg aaccacgcca caggcgtggg aaggtgggtt caaggtttcg ggttccgggt
     4921 tccggtagga acggccgttg tttcgcgtta cgggttcaag gtttcgcgtt tccctcgcgc
     4981 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnncac acactcaccc ctcacacact cacccctgaa cgctccccaa gttctccact
     5161 ttcctcagcc aattggccct gtagctgtct ttggaaagcc gaatggggcc gatggtagtg
     5221 gtgcgaaccg gacggatgcc aaggcgcctt ttttataggc ttccgccaat gcgaagttgt
     5281 agctctccgc gtcggggtga ccattgatga tgaggatttt atcagcgggc atgatttcca
     5341 tgattttctg ccatttccac cgccgcttta agcatcccca gcataccaat gctgaaaatg
     5401 agaaagccga aagacaattc ctgaagcgcc tgaatgtccg ttaaaaacat acccaccagg
     5461 cccattccaa taaagacgac tatcagaaag ctcaaaaaaa tcagaaaaat ccgggcatag
     5521 cgcgggttct cccagatgcg tttggataac ttgttttggg ttttggtagt atcctgaagt
     5581 gtggaaaatg aaactcccaa tcccagaaaa accagccctt tgttataatc tgcccagata
     5641 ttttcaaggc ctgacaatag tgggcgataa cagaaaaata tcgctgctgc catgatggga
     5701 tattacagat agctgatttt gtggaaaaag tctttggcgc ttttcatggc atatcatcgc
     5761 ttttgagcaa ggagaatcgc gtagtctctt cttgtacgtt cacgatgctt cgaaatctgc
     5821 gcggcgtatt ctgaaaatat tcaccggaaa acctttaaaa gtggtagttt tatcaagctg
     5881 cataccattt ttgcgggcca ccatttccga gcggatattt tcgacgtgga tgatggaaat
     5941 aatggaatcg gcaaaattat tttcaaaagc aaactccttg catttccggg ccgcttcggt
     6001 ggcataaccc cgattccagt aagcggatat gatggtgtag ccgatttcca attcctgcac
     6061 gccgtccact tcctgcacga gcagcccgca ttgccccact attttccccg aagccttgtc
     6121 cactaataca ttggtgccgc ccagatcgtt ttggtagcgg ttttccaccc gttgaaacca
     6181 ggtttcgcag cgttcttcgg cagtggaaat gcctgtcata ccgaggaatc gctccgtgtc
     6241 gggatgttcc atagcgggga gccattctgc aaaatcatcg cggtgctgcg gcctgaaaag
     6301 gaggcgctcg gattcctgct ggaggaggag gtatttcatt gagtatctgg tttgtgagag
     6361 ggttgggtca aacgttggtt ggtccaatcc gtaaaacttc tggctgcctg agcaaaatct
     6421 gaaggtacca gaacgatggt cgtggtgttg ttgtcaatgc cgatttccga aagcatttgc
     6481 atgcgtctca attccaaggc aatagggctg gcttccattt ctttcgcgcc ctgcgtcagc
     6541 ttgatggagg catccaattc ggcttccgcc tttacaattc tggcccgttt ttctctgata
     6601 gcttcggctt cccgcgccat ggctctttgc atgccttcgg gtatttccac atctttcatt
     6661 tctaccattt caatttttac cccccaaggc tcggtagatt catccacgat ttttttcagt
     6721 gtattgttga tttgctcccg gtctcggagc acttcatcca gcgtatgttg gcctatgatg
     6781 tttctcaaag cggataccga aaattggtaa acggcattgt tgtaattggc cactttgatg
     6841 atggcgcttt ccgggtcaat aattttaaac caaagtactg cattcacttt gatggtaacg
     6901 ctgtctctgg taatcgtttc ctgttgctcc aaatctactg ttcgggtacg tatgtccact
     6961 ttttgctgtc tttcaatcag cgggatgagc caataaattc cggggccttt gatacctgta
     7021 taacgcccca gtttaaaaat aatggaacgc tgatattcct gcgcgaccct gatacccgac
     7081 aacaggatca aagcggctaa cacgatgaat ggcatgtaga acatagatga tgcatttagg
     7141 catgataata ctgccgatac cattggatga atgcctggat accgtcttgt acgcttgtct
     7201 gtggtttata attcatttct ttcaccaaat cgctcacatc ggcataagtg gcgggtacgt
     7261 cgcccggttg tatgggctga aaatcaatga cggcgttgcg ccccagcgcc gcttcgatgg
     7321 cctgcacaaa atccatgagc cgcacgggat tctggttgcc gatgttgtac actttatacg
     7381 gtgcgcggct gctcgatggg tcgggttgca gtccgctcca gccggaattg cccgccggcg
     7441 gatggtccaa aacccgcacc acgccttcca caatgtcgtc aatataagta aaatcgcgga
     7501 tcatatcggc gtgattgaac acctgaatcg gctctccggc gaggatggct ttggtaaaca
     7561 aaaacagcgc catatcgggc cgcccccaag ggccgtaaac ggtgaaaaat cgcagcccgg
     7621 tagtgggcaa tccgaacaaa tggctgtaag tgtgcgccat gagttcgttg ctttttttgg
     7681 aagcggcgta cagcgatacg ggatggtcca cattgtcctg agtggaaaac ggcatttgct
     7741 cgttcaggcc gtacacgctg gaactgctgg cataggctaa atgtttgacc tcgttgtgcc
     7801 ggcaagcctc cagcaaattg gcaaagccca cgatgttgct gtctatgtag gctctggggt
     7861 tgaccaaact gtatcgcacc ccggcctgtg cagccagatt gcaggcggca tcgaagcgct
     7921 cggcggcaaa gagttcgttg agcgcggcgc tgtcttccaa tccagtttgt acaaagcggt
     7981 aattagggtg cagcgcgctt tgaaccaact ttccataagc gatttcatcc ttttggatgc
     8041 cggcatacgc caacctgttg tatttcagtt ggatatcgta gtagtcgttg agtacgtcca
     8101 atcctactac ttcatccccg cgcgctatga gccggttgat caaatgaaag ccgatgaagc
     8161 cggctgcgcc tgtgacgagt attttcatgg gaagtcagta tgcggtatgc ggtttggtta
     8221 atttctgatt tctgattttt gatttcgggt ttccctcgcg ctgcgccacg acgataggca
     8281 tggttcgcgg gatttcgcgt gtttcgcgtt gattcgcgtt aaagattttg acgttccggc
     8341 aggaacgggt ttcgcgttca aggttccgcg ttcaagcggt cgttttatgt aacttaccct
     8401 acgcgagacg acggttcaag gttccgggtt ttcgctcaaa tccgcgttcc gattttcgcg
     8461 ttccgcgcca cgacgcggct aacgcccaaa aggatgctcc gagagcggca acgccgccaa
     8521 tggatgaaaa tccccctcaa gcccgacggg aactgccttg cgaatattat gtaccgtgcg
     8581 aatggcaatc cctgcttctt ccaggccgaa acccttaccg gagagaatat cctgataact
     8641 cagcgtatgc aaatcggtga agccctcgct gaattcgagt tcctgcccgt ccaccgtgat
     8701 agagcggaaa gtgcgtccgc ctttggcctg cacttcggcg ggcagcgtat cggcattgat
     8761 gctcagaaac cagcgcacgc gagcgcgttc caattccaaa aagccggcag cgcggtcgtg
     8821 ggtatgcaca tggactacat tttgccgtac cggcccgaag acccattgca gcatgtcgta
     8881 aaaatggatg ccgatgttgg tagcaatgcc gcccgacttg ttgaggtcgc ccttccagga
     8941 agtgtagtac cacttgccgc gcgaggtgat gtagctgagg tccacctcgt gttggagcag
     9001 ggtaggggag gactgcactc gctcccgcaa ctcaatgatg gccggatgca ggcgcaattg
     9061 caggatggta taaactttgc gcccggtttc gcgctccata tcctgaaggg cttccacgtt
     9121 ccagggattg agcaccagag gtttctcaca gatgacgtcg gcgccgaggc gcaaaccgaa
     9181 gcggatgtgc gcgtcgtgga ggtaattggg cgagcaaact accacataat caatgtgttg
     9241 tccgctgcgt ttcagttttt ccaggtgccg gtcgaagcgc tcgaattccg taaaaaaatc
     9301 ggcctgcggg aaatggctgt caatgacgcc taccgaatca ttgggatcat aggctgccaa
     9361 caattgatgg ccgctgtctt tgatggcgcg catgtgacgg ggcgctacaa agccggccgc
     9421 gccgatgagg gcaaaattca tggtgatgga ataatggata caataatttg ttaagcagaa
     9481 ggcttttagc cgttagctta tggccactgg ctggttttcc ctgatataaa tacaccatag
     9541 atcatggggc aacagctaac agctataagc taacggccag cagccaaagc cttacttctc
     9601 ctctgcggcg tcttccactg tttcaccctc cggccactgc gtgatgtgcg cctccttttg
     9661 ccgccagaga cctttattgg ttttcagtcc cttgcggttc acattcttct cctcaatggc
     9721 ttcggcatag gtggcaaagt agtcgcttgt gcagttggta aaggtgcagt tgttcagaga
     9781 gaccgtttgg ttgtcgtcct gaaatctgaa gaattcataa gggccgtatt ctttgggcgt
     9841 ctggttgttg atgaattggc agttattgaa cttcaaacca tacgcgaatt gcagtttgac
     9901 catattatcg ccggtattgt ctttgaaagt acaatcatca aaaacgaaac tattgctgct
     9961 catcaaggat aagatgccgt aagtacactc ccgaatgacc gagcgttgca tggtcagaaa
    10021 ggaaacgcct tccgcttcta ttcccatggt gccgctgccg tacagatcac agttttcaat
    10081 atggaggtaa ttggcgttga gtaccgtcat gacggcgcct tcgcacaatc ctttctgtac
    10141 atcgtgaccc atagtgatgc ctgagaagga gatattgaag ccgccctcta ttttaaacac
    10201 aggaatgaat gcgtaaggaa tcacccattc cactttgcct ttgtttttgg ctacgaaatg
    10261 caggtttttc acgtccttga tgcgtatggt gcggtcttcc atttggtatt ccgagaaatc
    10321 gaaaatatcg gcaaattcag agccgccgtt taccgtgctc aggtcgtact tcccatcttc
    10381 gagggtgatt tccacgtcgt tgccgatggc gtcgaacagt gcctgcgcgt tattgaccgt
    10441 cacttttcaa tggggcgtct ttggagtaga tggctccgtc ggggccgatg tagctctccc
    10501 gcccgttgat atcggtagca taagtgaggc cgaaagcgtc aggtacgcca cagaatgtcc
    10561 attgtttttc tgagattttt ttgccgttta aattgtaaag atgccctttg ccgccttttt
    10621 gaaacaggat ggcggtataa ggtctttcca tagcttctat gtactcgccg ccttcaccgt
    10681 gtgtgggcat atcaaattct atgggcaaca aagttttgcc cttgtcatcc agcagcccgc
    10741 gtttgccgtt tttatcaact tgaaaacgtc ctggcgccac tacctctaca tggtcgtatt
    10801 ccaggggcag caacacttgg ttggttgtgc tgatgatgcc cgttttgcct tcccctttcg
    10861 aggcgatgag gtagtttctg gatgataaca gatagttata ctcaaaaggc accaagactt
    10921 tgccggtatt gtccaaaagc acc
//
LOCUS       DAORLF010000112        10923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3228_length_10923_cov_2.127254, whole genome shotgun sequence.
ACCESSION   DAORLF010000112 DAORLF010000000
VERSION     DAORLF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_10923_cov_2.127254"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>214
                     /locus_tag="PK198_10675"
     CDS             <1..>214
                     /locus_tag="PK198_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39245.1"
                     /translation="YHGRETWRLKQLSEYFPQYPASLRRFVPEFDKIFISLFDVSPEH
                     IEQLTDAMLQAALMVQRNRYHSKELVR"
     assembly_gap    215..236
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <237..572
                     /locus_tag="PK198_10680"
     CDS             <237..572
                     /locus_tag="PK198_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39246.1"
                     /translation="TLLPQSEYSNFLTTISVYSISLIDLKPEELRAEILKHSRQKKST
                     AMSTYDQLIQKGEEQGIQKGKIEGKIEGKIEVALNAYNNGLSIQLIANITGMEEAEVS
                     RILKENGMI"
     gene            669..>1348
                     /locus_tag="PK198_10685"
     CDS             669..>1348
                     /locus_tag="PK198_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRF39247.1"
                     /translation="MTTNQKIQALRQAMQREGIAAYFVPSSDPHQSEYVADHWKCREW
                     LSGFTGSAGVLVVTEDHAGLWTDSRYFLQAEKDLAGTCVTLQKQVVPHAPEHVNWLAD
                     SLPPGSIVGIDGRLFSLAQVRHLAKHFYPKNIEIRTDLDLIAPIWINRPALPGDPVFE
                     LPPDYTGHSLAEKLARIRAALGAQNADALLLTTLDDIAWTLNIRAADVECNPVCISYL
                     LIEQEAPD"
     assembly_gap    1349..1358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1359..2460
                     /locus_tag="PK198_10690"
     CDS             <1359..2460
                     /locus_tag="PK198_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HRF39248.1"
                     /translation="SAEKVPLELQEKCWLHGIVLHPYEAIEDRLHTLPPDYKVLSDTA
                     TLNARLYQLIPEGQILHGDNPVAAMKGIKSEREIAQIRRAMRKDGAALVRLLRWLETE
                     VPKGGLTEYDVAVKMAELRAEQGEYFGESFAAIIGYNANGAIVHYRPMPGESAAIRPQ
                     GILLMDSGGQYLHGTTDITRTIALSPPTGEQKRAYTAVLKGHIALAALRFPEGTKGIQ
                     IDALARVPLWQLGLNYGHGTGHGVGYFLSVHEGPQGITPSPGNSKGHTPFEPGMLTSN
                     EPGYYETGQYGIRIENLILCVEAEQTASGRFFQFETLSLCPIDTQLIDISMLHPEERQ
                     WLNEYHAGVLRELSPLLHAEEVAWLEQQCRAV"
     gene            complement(2575..>2809)
                     /locus_tag="PK198_10695"
     CDS             complement(2575..>2809)
                     /locus_tag="PK198_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39249.1"
                     /translation="VLFGTGGGISNYDPKEKTTMKYISNYTLRIHNQADTTRTDKQIL
                     KEMLETIEIKSTAIDTLPESEKYQLLRTMFKGG"
     assembly_gap    2810..2819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2820..3941)
                     /locus_tag="PK198_10700"
     CDS             complement(<2820..3941)
                     /locus_tag="PK198_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39250.1"
                     /translation="MEEIDLKEWQWLLRKFCESRKLSRMEEIYISEKLKTLTNIQTNT
                     VDPNGYAYEGLVEVICTHIPNARDKDVLNGKRINELYHRSKNKDLGDGLKGWYRPELF
                     NYLYQFIAISENKEGIRTRRDYLEQAPEIILENYTEEELTDILKDKTGKNNGTEDEQT
                     TAEKRAKTEAEDKAKAFLRLLQLKMQQNAQMPAPEPPAVEVSLISKKPDAPLEALQAL
                     RHRLRQLGLLAVVFGLSTILLGALYFSERKSHQTQGIPHINYAAFKGDSIGQFNLQFR
                     LKDTLDGLWEYPTLTFYKLKNDPKNNDWFIVEMEGLYRSNDGGSGPNTTRHIGIARKH
                     DSFIQFELEPIDAKEMNDYGKWYATIYHAGASFPDVLPGK"
     gene            4105..4398
                     /locus_tag="PK198_10705"
     CDS             4105..4398
                     /locus_tag="PK198_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39251.1"
                     /translation="METLKKIDEFFEVIGEIFGFLTDHVFPIIGVIWLGYLFLKSLGA
                     YIEDWSWLGVLLCLLHWAAGPLLIYFGVEFGISILLGCGGLVLLSALATGAED"
     gene            4409..4915
                     /locus_tag="PK198_10710"
     CDS             4409..4915
                     /locus_tag="PK198_10710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF39252.1"
                     /translation="MNTQILAQRLAAWRQEPDYCHFTEAELIRIFYDALDDLPCSVHF
                     TIGEAAGIVASARLSLITPDNLTLLPYGNFQTVLSGFLPSFGLLGRLAVLPEYRGNGF
                     ATELTQRRISYARRHFPHLILLTLLRPCAISQYADLGFQDFLSYSKNDYPGLPWTETV
                     LAIFSPSK"
     gene            4973..>6193
                     /locus_tag="PK198_10715"
     CDS             4973..>6193
                     /locus_tag="PK198_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRF39253.1"
                     /translation="MQNVSESDRGEFQLPAFARAAAASQQQQAIQTWLELNDLAERSW
                     AEERTGVYVEARARKLGIPVTQLPGSHTRIVRIKGTGNGPYKGVLAYKADLDALPYKN
                     SEGKVYYKHSCFHSGHVAIALSVMETVWELRHRFNSEVIFIYQTAEETPDCGARELLN
                     SSLWKELQIDRMVALHASPELRFDQVAVREGYAQAGIDVLTFKVSVDTAKIKSSHVAR
                     PHAGANTIWAAMKLATQMKFALSETSNPVQPLLFNIVQIGSSNFGMDNVNITPDETTF
                     IVNLRVFDLNFRNELMQKIERMVQSVELEYNGVIQVEMQVERGPDPVFNNPALARKVF
                     QYAQEVLGEDHVQVAEQRAGGDDAGVYSQVIPLVMVRLGTGNPDKGIVTDLHNERFTV
                     DTDCFFTGIAAMTYV"
     assembly_gap    6194..6302
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6406..7737
                     /locus_tag="PK198_10720"
     CDS             6406..7737
                     /locus_tag="PK198_10720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRF39254.1"
                     /translation="MAQVELIMPKMGESIMEATILRWVKNPGDSVEIDEPILEIATDK
                     VDTEVPSPVSGVIAQVLFKENDVVPVGKVIAVISTEGESAQPLPTPSIAEAPASNGAL
                     SNGQHSPAPAEAVPATASSAPAQVRAAGEGARFYSPLVRNIAQKEMISTQELEAVPGS
                     GQQGRVTKKDILAYVEQRRHTPATPQPAAPAPATPASVPAAPAVSVSGNVEIVEMDRM
                     RKLIADHMVMSKHTSPHVTSFVEVDVTEIVNWRERVKKQFEARYGEKITFTPIFIEAV
                     TKALRDFPMVNISVDGTKLIVKKDINIGMAAALPSGNLIVPVIKNADLLSLPGLTKAV
                     NDLAGRARANQLKPEEIQGGTFTLTNVGTFGNVMGTPIINQPQVAILATGAIRKKPAV
                     VETPYGDLIAVRQMMFLSLSYDHRVVDGALGGSFLRRVGDYLEQFDANRGF"
     gene            7734..8699
                     /locus_tag="PK198_10725"
     CDS             7734..8699
                     /locus_tag="PK198_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF39255.1"
                     /translation="MTRPMPSPRILLIDRPHGFLIEALQNWGAELSLHYETPREEVLR
                     LLPEYDGVVMRSRLRADAEFLQQGRRLRFLAREGVGVEHIDLDCAAQLGIEVITSPEG
                     SRNTVAEHTLGLLLGLMNHLARADRQVRQGEWLREPNRGYEIFGRTVGILGYGNMGSA
                     FAQRLAGFGCRVIAYDKYKTDYGNEYAEAVDLPTLQRESDILSLHIPYMPANHYFVNE
                     VFLSAFSKNIFVVNTARGTVLETEALVRHLQSSHVLGAALDVLEYEETSFEFLNFPAL
                     PAAFQYLRQADNVVLSPHIAGWSFESKLGHAQVLAEKIQAFWKGR"
     assembly_gap    9110..9182
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9183..>9829)
                     /locus_tag="PK198_10730"
     CDS             complement(<9183..>9829)
                     /locus_tag="PK198_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRF39256.1"
                     /translation="IEQYGCCNEDAKTKNVLITDANRSDVNTSNKPDGSFRMNTFWSS
                     SQYEMNKVGSYIFVEDDKGFQWYARSQNPINDDIPLARCRCISREKGDIQLIPLITPD
                     KKDSQKGIMTDLRDGRIYSYKVMKDGKKWMTKNLNFEMADSYCYDNNEVYGEEFGRLY
                     SWKAALKACPKGWHLPSDEEWISLANSYGGGYRDFETEQYIGDATVTFNALMEGG"
     assembly_gap    9830..9839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9840..10142)
                     /locus_tag="PK198_10735"
     CDS             complement(<9840..10142)
                     /locus_tag="PK198_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRF39257.1"
                     /translation="MNLRLQINFIIFLACAYALVGCDAPPKKIYTDSRDGQTYSYTVL
                     ENGTKWMTQNLNYDAQNSICGENDKSCKHGRFYSWEDLKEACPTGWHVPNHLEWKYF"
     gene            complement(10446..10802)
                     /locus_tag="PK198_10740"
     CDS             complement(10446..10802)
                     /locus_tag="PK198_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF39258.1"
                     /translation="METTIWVLKAATALLFIFVGINKLMLPKAKLLDKGMKGLINLDE
                     KQIKTAGVLEILGAIGLILPSALNYFPALSSISALCLGLTMIVAGMINYKLKLSIIPN
                     IVIFAVCIFIAYWGIK"
BASE COUNT         2562 a         2966 c         2513 g         2648 t
ORIGIN      
        1 attatcacgg cagggagacg tggaggctca agcagctctc ggaatacttc ccccagtatc
       61 cggcatcatt gcgccgtttt gtgccggagt ttgataagat attcatcagt ctgttcgatg
      121 tatcgccgga acacatagag caattgaccg atgccatgct tcaagccgca ctcatggtac
      181 agcggaatcg ctaccattcg aaagaactgg tccgnnnnnn nnnnnnnnnn nnnnnnaccc
      241 tgttgccgca atctgagtac agcaactttc tgacaaccat ctctgtttat tccatcagtc
      301 ttattgactt aaaaccggaa gaactcagag cggaaatact caaacattca agacaaaaaa
      361 aatctacagc catgtcaacc tacgatcaac tcattcaaaa aggagaagag caaggcatcc
      421 aaaaaggtaa aatcgaaggc aaaatcgaag gcaaaatcga ggtggcgcta aatgcctata
      481 acaacggcct ctccatacaa ctcatcgcca atatcaccgg catggaagaa gcggaagtaa
      541 gccggattct gaaagaaaac ggcatgatat aagtctcctc tgttcttgcg cccgtctttt
      601 tgacccgacg catcttcaac ctgccgcttg ttacgcgccg tacaaattcc gtcactttcc
      661 caatcaaaat gactaccaac caaaaaatcc aggcgctccg ccaggccatg cagcgggaag
      721 gcatcgccgc atacttcgtc ccttcttccg acccgcatca aagcgaatat gtagccgacc
      781 actggaaatg ccgcgagtgg ctctccggct tcaccggttc cgccggcgtt ttggtcgtca
      841 ccgaagacca tgccggcctc tggaccgact cccgctattt cctccaggcc gaaaaagacc
      901 tcgccggcac ctgcgtcacg cttcaaaaac aggtggtgcc gcacgctccc gaacacgtca
      961 actggctggc cgacagcctc cctcccggca gcatcgtggg cattgacggg cgcttgtttt
     1021 cgctggctca ggtacgccat ctggctaagc atttttatcc caaaaatatt gaaattcgca
     1081 ccgatctcga tctcatagcg cccatctgga tcaaccggcc cgcactgccc ggcgacccgg
     1141 tattcgaact gccgcccgat tacaccggcc actcgctcgc cgaaaaatta gcccgcatcc
     1201 gcgccgctct gggtgctcaa aacgccgatg cgctgctcct cactaccctc gacgacatcg
     1261 cctggacgct caacatccgc gccgccgacg tggaatgcaa ccccgtctgc atctcttacc
     1321 tgctcatcga gcaagaggcc ccggactgnn nnnnnnnnat ccgccgaaaa agtgccgctg
     1381 gaattgcagg aaaaatgctg gttgcacggc atcgtcctgc acccttatga agccattgag
     1441 gacagactgc acaccttgcc tcccgattat aaagtgcttt ccgacaccgc cacgctcaac
     1501 gccagattgt accaactcat ccccgaaggc cagatattgc acggcgacaa ccccgtagcg
     1561 gccatgaaag gcatcaagtc agaacgcgaa atagcccaaa tccgtcgcgc catgcgcaaa
     1621 gacggcgccg cgctcgtgcg cctgctgcgt tggctcgaaa cagaggttcc caaaggcggc
     1681 ctcaccgaat acgacgtggc cgtcaaaatg gccgaactcc gcgccgaaca aggcgaatac
     1741 ttcggcgaaa gcttcgccgc catcatcggc tacaatgcca acggcgccat tgtacactac
     1801 cggcccatgc ccggcgaatc ggcggccatt cggccacagg gcatcctgct gatggactcc
     1861 ggcggccagt atctacacgg caccaccgac atcacccgca ccatagccct ctctccgccc
     1921 accggcgagc aaaaacgggc ctacaccgcc gtcctcaaag ggcacatcgc attggctgcc
     1981 ctccgcttcc ccgaaggcac caaaggcata caaatagacg ctctggcgcg cgtgcctctc
     2041 tggcaactcg gcctcaacta cggccacggc acagggcacg gcgtcggcta tttcctcagc
     2101 gtacacgagg ggccgcaggg catcacgcct tcgcccggca acagcaaagg ccatacgcct
     2161 ttcgagccgg gcatgctcac ttccaacgaa cccggctatt acgaaaccgg ccaatacggc
     2221 attcgcatcg aaaatctgat cctgtgcgta gaggccgaac agacggcttc agggcgcttt
     2281 tttcagttcg aaacgctctc cctttgtccc attgatactc aattgataga catctccatg
     2341 ctccatcccg aagagaggca atggctcaac gagtaccacg ccggcgtact gcgcgaactg
     2401 tcgccgttgc tccatgcaga agaagtggct tggttggagc agcagtgcag ggcggtgtga
     2461 tcgaggtttt attggggcgt gcccccacac tgataccatc ttcttgtggg gtcgggtgcc
     2521 ttggtcgtcc tcctgtcgtc gttcctccct tcggccccct cacgcaacgc gcgttcagcc
     2581 tcctttgaac atggttctca atagctgata tttctccgac tcaggcaaag tgtcaatagc
     2641 ggttgatttt atttcgatag tttccagcat ttccttgaga atttgcttgt ctgttcgggt
     2701 ggtatctgcc tggttgtgaa ttctgagcgt gtagttggag atgtatttca tcgtggtttt
     2761 ttcctttgga tcgtagttgg agattccacc gccggtacca aataaaaccn nnnnnnnnnt
     2821 ttccccggca acacatccgg aaacgacgct cccgcatgat aaatggtggc ataccatttt
     2881 ccataatcat tcatttcttt tgcatctatc ggctccaatt caaactgaat aaaagagtcg
     2941 tgttttcggg caatgccgat gtgcctcgtg gtattaggcc cgctgcctcc atcgttcgat
     3001 ctgtataagc cctccatttc cacaataaac caatcgttgt ttttgggatc gttttttagc
     3061 ttgtaaaaag tcagcgtcgg gtactcccac agaccgtcca gtgtgtcttt gagccgaaac
     3121 tgtaaattga attgcccaat cgaatcgcct ttgaacgcgg cgtaattgat gtgtggaatc
     3181 ccctgcgtct gatgcgattt gcgctcggaa aaataaagcg cccccagcaa aatggtactt
     3241 agcccgaata caaccgccaa taagccaagc tgccggagac ggtggcgaag ggcttgcaag
     3301 gcttcgagcg gtgcatccgg cttttttgat ataagagaaa cctcaacagc aggcggctca
     3361 ggcgcaggca tttgagcatt ctgctgcatt ttgagttgaa gcagccttag gaaggctttc
     3421 gctttatcct cggcttccgt tttggccctc ttttctgcag ttgtctgctc gtcctctgtt
     3481 ccattgtttt tgccggtctt atctttcaag atatccgtca gttcttcttc tgtatagttt
     3541 tccagtatga tctccggcgc ctgttcaaga taatctctgc gagttcggat gccttcttta
     3601 ttttcagaaa tggctataaa ttggtacaaa tagttgaaca gctcaggccg gtaccaacct
     3661 tttagaccat cccccaaatc cttattttta gaacgatgat acaactcgtt gattcttttc
     3721 ccattgagca cgtctttgtc gcgagcattg ggtatatgtg tacaaatgac ctcaaccaag
     3781 ccttcataag catagccgtt ggggtccacc gtattcgttt gtatgttcgt aagggtcttc
     3841 aatttttcgc tgatgtatat ctcttccatc ctgctcaatt tcctggattc gcagaacttc
     3901 ctcagcagcc attgccattc tttcaagtca atttcttcca tttttatgct tctttttact
     3961 tctgattaca tgaattttca ccccattgca tgaatttctt ccattgtagc agcaatttcc
     4021 ttccaatacc cacactggct gagcgccgcc tacacatttg cagcacgagg caatttcgct
     4081 tcgcgtcgca aaatcgctgt tttcatggag accttgaaaa aaattgatga attcttcgaa
     4141 gtcattgggg agatatttgg cttcctgacg gatcatgtgt tccccattat cggggtgatt
     4201 tggctcggct acctgtttct taaatccttg ggggcataca tcgaagactg gagctggctc
     4261 ggcgtgctct tgtgtctgct gcactgggca gccggccccc tcctgattta ttttggggtt
     4321 gaatttggca tttctattct attgggttgc ggcggccttg tcttattatc ggcccttgcc
     4381 acaggggccg aggattaaac gagccgccat gaatacacaa atcctcgccc aacgccttgc
     4441 cgcctggcgg caggagccgg actactgcca tttcacggag gccgaactga tacgcatttt
     4501 ctatgatgcg ctggacgacc tgccctgttc cgtccatttc accattggtg aagcagcggg
     4561 cattgtcgcc tcggcccggc tctcgctcat aacgcccgac aacctgacct tgctgcctta
     4621 tggcaatttc caaaccgtcc tctccggttt cctgccctca tttgggttgc tgggccgctt
     4681 agctgtattg ccggaataca gggggaatgg ttttgctacc gaactgacgc aacgccgcat
     4741 cagctatgcg cggcggcatt ttccgcacct tatactgctc accctgttgc gcccctgcgc
     4801 catatcgcaa tatgcagatt tgggttttca ggacttcctt tcgtattcca aaaacgacta
     4861 tcccggcttg ccctggacag agaccgtgct tgctattttt tcaccttcaa aataactgat
     4921 tatgaggtat ttattgcttt ccttttgttc actttttcta aattcttcca acatgcagaa
     4981 tgtatcagaa tccgatcgcg gggagttcca actgcctgcc tttgcccgcg ctgcggctgc
     5041 ctcgcaacag caacaggcca tccagacatg gcttgaactc aacgatctgg cagagaggtc
     5101 ttgggcggag gagcgaactg gggtctatgt cgaagcgcgc gcccgcaaac tgggcattcc
     5161 cgtgacgcaa ctgcccggct cgcacacgcg cattgtgcgc atcaaaggta ctggaaacgg
     5221 accctacaaa ggggtgctgg cctacaaagc cgaccttgat gctctgccct acaaaaactc
     5281 cgagggcaag gtttactaca aacattcttg ttttcatagc gggcacgtcg ccattgcctt
     5341 atcggtaatg gaaaccgtgt gggagcttag gcatcgcttc aattcggagg tcattttcat
     5401 ctatcagact gccgaggaga cccccgattg cggcgcacgg gaacttctga actcctcgct
     5461 ttggaaagaa ctgcaaatag atcgtatggt ggcgcttcat gccagcccgg aactgcgttt
     5521 cgatcaggtg gccgtcagag aaggctatgc acaggcaggc attgatgtgc tcactttcaa
     5581 agtatcggtg gacacggcaa agatcaagtc cagccatgtg gctcgtcctc acgcaggcgc
     5641 caacaccatt tgggctgcta tgaaattggc cactcaaatg aaattcgccc tttcggaaac
     5701 cagcaacccc gtgcagccgc tgctttttaa tatcgtgcaa atcggcagct cgaattttgg
     5761 gatggataat gtcaacatta cgcctgacga aacaactttc attgtcaatt tgcgcgtatt
     5821 cgatctgaat ttcagaaatg aactgatgca aaaaatcgaa agaatggtcc agtctgtgga
     5881 gttggaatac aacggcgtta ttcaggtgga aatgcaggtg gagcgcggcc ccgaccctgt
     5941 gttcaataac ccggctctgg cccgaaaagt gtttcaatac gcccaggagg tgctgggcga
     6001 agaccatgtg caggtggccg agcagagggc cggtggagat gatgcggggg tctatagtca
     6061 ggtaattccg ttggtgatgg ttcggttggg tacgggcaac ccggataaag gaatcgtcac
     6121 cgatctgcac aatgagcgtt tcacggtgga cacggattgc ttttttaccg ggatcgccgc
     6181 catgacttac gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nntcttcttt ttcagaccgt tgcaggtctg aacctgccta aatgaaaaat ccataccttt
     6361 gcccccgaat caaataacca aattgaaacc gaaataaaac gcatcatggc acaggttgaa
     6421 ctcatcatgc ccaaaatggg cgaaagcatc atggaagcca ccatcctgcg ctgggtcaaa
     6481 aatcccggcg acagcgttga aatagacgag cctatcctcg aaatagccac cgacaaagtg
     6541 gacaccgaag tgccctcgcc cgtcagcggg gtcattgcac aggtgctttt taaagaaaac
     6601 gacgtagtgc cggtgggcaa agtcatcgca gtcatcagca ccgaaggcga atcggcacaa
     6661 cccctgccca cgccgagtat tgcggaagcg cccgcatcca atggcgccct cagcaacggc
     6721 caacacagcc cagcgcccgc cgaagctgtg cctgctaccg cctcttccgc tccggctcaa
     6781 gtgcgcgctg ccggcgaagg cgcccgcttt tactcgcctc tcgtgcgcaa catcgcccaa
     6841 aaggaaatga tcagcactca ggaattggaa gcagtgcccg gcagcggaca gcagggcaga
     6901 gtgaccaaaa aagacatctt agcttatgta gaacaacgcc gccacacgcc cgccacgcca
     6961 cagcctgccg cgcctgcgcc cgccactccg gcatccgtac ccgccgcgcc cgccgtatcg
     7021 gtgagcggca atgtggagat tgtggaaatg gaccgcatgc gcaagctcat cgccgaccac
     7081 atggtgatgt cgaaacacac ctcgccgcac gttacttctt ttgtggaggt ggacgttacg
     7141 gaaattgtga actggcgcga gcgcgtgaag aaacagttcg aggcccgcta cggcgaaaaa
     7201 attaccttca cgcctatttt tattgaggcc gtgaccaaag ccctccgcga tttcccgatg
     7261 gtcaacatct ccgtggacgg caccaaactc attgttaaaa aagacatcaa cataggcatg
     7321 gcggcagcgc tgcccagcgg caacctcatt gtgccggtca tcaaaaacgc cgacctgctc
     7381 agtctgcccg gcctcaccaa agccgtcaac gatctggcgg gccgcgccag agccaaccaa
     7441 ctgaagcccg aagaaattca gggcggcact ttcaccctca ccaatgtggg gactttcggc
     7501 aatgtcatgg gtacgcccat catcaatcag ccgcaggtgg ccatcctcgc tacgggcgcc
     7561 attcgcaaaa aaccggccgt ggtagaaact ccctacggcg acctcattgc ggtgcggcag
     7621 atgatgttcc tctccctctc gtacgatcac cgcgtagtgg acggcgcgct cggtggctcc
     7681 ttcctgcgcc gggtaggcga ttatctggag cagtttgatg ccaatcgggg gttttgacgc
     7741 gccccatgcc atcacctcgc atcctcctca tagaccgccc gcacggcttc ctcatcgaag
     7801 cgctgcaaaa ctggggtgcg gaactgagcc tgcactacga aacgccacgc gaagaggtgc
     7861 tgcgcctgct gcccgaatat gacggcgtgg tcatgcgcag tcgcctgcgc gccgatgcgg
     7921 aatttttgca acaaggccgc cgactccgtt ttctggcccg cgaaggcgta ggcgtagagc
     7981 acattgacct ggactgcgcc gcccagctcg gcatagaggt catcacctcg cctgaaggca
     8041 gccgaaatac ggtggccgaa cacacactgg gcctgctcct cggcctgatg aaccacctgg
     8101 cgcgcgccga ccggcaagtg cggcagggcg agtggctgcg cgaacccaac cggggctacg
     8161 aaatctttgg cagaacggtg ggcattctcg gctacggcaa tatgggttcg gcgtttgcac
     8221 agcgtttggc agggttcggt tgccgcgtca ttgcctacga caaatacaaa accgactacg
     8281 gaaatgaata tgccgaagcg gttgacttgc ccaccctgca acgcgagagc gacatcctca
     8341 gcttgcacat tccatacatg ccggccaacc actattttgt caacgaggtc tttctgagtg
     8401 ccttttccaa aaatatcttt gttgtgaata ctgctcgcgg taccgtactc gaaaccgagg
     8461 cattggtgcg gcatctgcaa agcagccatg tactcggcgc ggccctcgac gtgctggaat
     8521 acgaagaaac ttcctttgaa tttctcaatt tcccggcttt gcccgccgct tttcagtatc
     8581 ttcgccaagc cgataatgtg gtgctctctc cgcatattgc cggctggagt tttgagtcga
     8641 aactggggca cgcacaggtg ctggcagaaa aaattcaagc cttctggaag gggcggtgaa
     8701 gatgctttta ttattcgtta ccattcagcg ccctgctaaa agccatgagc ttcgaccacg
     8761 cctcggcgtg gcgcgagctt gattttccaa aatgaaaaaa agcactcgtt ttcagaggga
     8821 tattttgaaa tttccccaaa tggaaaatct tatttttgaa caaaaaaaca accacgcagg
     8881 gcgtggtcgt ggcttgcagc ttgtagcttt tggagacccg ctgaatagtt accaaattta
     8941 aaatgaaaaa attaattttc tacacatctg caagaaagtc ccatcagtat agagctgtca
     9001 tttcttcgaa aagaggtgac gtcaccaagc tggaaattcc aggcttcatc tttattttgg
     9061 gttgagacac tactgctcca ataacttgca acccggaaaa tctgtttaan nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nntctcctcc ttccatcagg gcattgaacg tgaccgtagc atcaccaatg tactgctctg
     9241 tttcaaagtc tctatatccg ccaccatagg aattagccaa tgaaatccat tcttcatcag
     9301 agggcaaatg ccatcctttt ggacatgctt taagcgcagc tttccatgaa tagagccttc
     9361 caaattcttc gccatatact tcattattat cataacagta tgagtctgcc atttcaaaat
     9421 ttaaattctt tgtcatccat ttttttccat ctttcatcac tttataagag tatattcttc
     9481 catcccgtaa atctgtcata atcccttttt gcgaatcctt tttgtccggc gtaatgagag
     9541 gaataagttg aatatctccc ttttcgcggc ttatacatcg gcaccttgca agtggtatgt
     9601 cgtcgttgat tggattttgg ctacgggcat accattgaaa acccttgtca tcctctacaa
     9661 aaatataaga cccaacttta ttcatttcat attgtgaact actccaaaaa gtattcattc
     9721 tgaaagaacc atctggttta ttagaagtgt tcacatcgct tctattggca tctgtaataa
     9781 gaacgttttt cgtcttagca tcctcattgc aacagccata ttgctcaatn nnnnnnnnna
     9841 aaatacttcc attccaagtg gttaggaaca tgccagcctg ttgggcatgc ttcttttaag
     9901 tcttcccaag agtaaaaacg gccatgttta catgatttat cattttcgcc gcatatggag
     9961 ttttgcgcat cataattcaa gttttgagtc atccactttg taccattttc caaaacagta
    10021 taactatagg tttgtccatc cctgctatct gtatatattt ttttaggtgg tgcatcacag
    10081 ccaaccaaag cataagcaca agccaaaaaa atgataaaat taatttgtaa tcgtagattc
    10141 attcgtgttt tattttgtgg tgttgactaa tgtagtggac aaagtaagtt aaacggagtc
    10201 gctttacccg acacaactac gggcaaaaca atggtctatg gatttgtaac agtccttttg
    10261 attcggcaga caaaatacaa acttatggca caccaaccaa gccgggacgc gaaaaaaaat
    10321 gcgggggaaa gtgcatctgc tcataaaaac gacgggtata gtccttgcgc cggggcgcgc
    10381 tgcgcgcgtt tataccccca cccgcaggcg ggtttgctgt tgtgccctaa atgttagtag
    10441 ttgccttatt ttatgcccca atacgcaata aaaatacaga ctgcaaatat gacaatattg
    10501 ggaatgatag aaagtttcag tttgtagttt atcatccctg caacaatcat tgtcaagccc
    10561 aagcacaaag cagaaataga ggatagcgcg ggaaaatagt tgagtgcaga aggcaaaatc
    10621 aaacctatcg cccctaaaat ttccaatacc cctgctgttt taatttgctt ttcgtcaagg
    10681 ttaattaatc ctttcattcc tttgtcaagc agtttagcct ttgggagcat taatttgtta
    10741 atccccacga aaatgaacag caatgctgtg gccgctttca atacccaaat tgttgtttcc
    10801 attttagatt tgttatttat tcgttttatt cagttttgta tctgccaatg tcgttacttc
    10861 acttgccgct gacgttttgg cgcttggcgc agtggcggat ttcggagcac aaaactgtca
    10921 ata
//
LOCUS       DAORLF010000113        10841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3256_length_10841_cov_2.184220, whole genome shotgun sequence.
ACCESSION   DAORLF010000113 DAORLF010000000
VERSION     DAORLF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_10841_cov_2.184220"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PK198_10745"
     CDS             <1..1294
                     /locus_tag="PK198_10745"
                     /inference="COORDINATES: protein motif:HMM:NF026241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF39259.1"
                     /translation="LTDDAHKNMQFDYNFLNLALNITTTQGTLHFLYDATGRKWAKSG
                     PDGIRFYAGNIEYLDGKIQHIAFPDGRLVAEYNTSGTSVTGYRSEYFRTDHLGNTRLT
                     FSDFNQDGLISWKLPTAGPAAQESEITSENHYYPFGLSQKGPWYASVAPENKYRYNGK
                     EWNDEWGLGLYDFGNRWHDPTLGRFTTVDRFAEKYAFQSAYVMAANDPMKFVDVNGDS
                     IFIRTGKGKENIALYDNGVVTNRDGTKYTGKGVKERKDGSVKLQGFLKQTVNALDKIR
                     SGGPVGSNLVSTLEKDNDIFVITKGPVNDARGRAVHFNPASFEAGLDVNGSRNTSPFV
                     SLAHELAHALDWDNGSIDTRTWITYSDNKTTSTRAEIYASHVENQIRAENNLPLREFY
                     GIDQGKGVGRLLIPGTRMSAHYMQTQKVPTLIQIPFKY"
     gene            1299..1787
                     /locus_tag="PK198_10750"
     CDS             1299..1787
                     /locus_tag="PK198_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39260.1"
                     /translation="MKSVFLCVFLLMQGGIYSQIGSVIEFIDPSLFKGKGVIFVENLS
                     PPIQLPKNAKIINLTKEQVYISELIFLNQFSQNATLAFFNNKNIKKYFWYYNRQYLGF
                     KDENGRINVIINLLNFRNKKIAEKKFEGWGEEYFIGFGEYYEKNTLRISVNIDLCKVD
                     VW"
     gene            2099..>3200
                     /locus_tag="PK198_10755"
     CDS             2099..>3200
                     /locus_tag="PK198_10755"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRF39261.1"
                     /translation="MIEQLRATHAAAAQIRRLSDAAKTKLLLQLAQAVEAACDAILAA
                     NQLDLAKIDDADPKKDRLLLNPERIAALAGSIREVAALRDPCGAVLLDKILPNGLKLR
                     KISVPLGVVGIIYESRPNVTLDVSALCLRSGNAAVLRGGSDALQTNRALAAIIQDVLE
                     QAGLSRDVVALLPPDRELVKDMLTATRYIDVIIPRGSDALIRFVRENSLVPVIETGAG
                     VCHTYVHESADLEKAKAIVVNAKVSRPSVCNSLDTIVIDSKIAPAFLPLLRDDFEKWK
                     VEVFADPVAFDILQQAGFERLQPASESDFGREFLDYKCSVKTVNGLPEALDHIRTYSS
                     RHSEAIVAEDTAVCRTFLEETDAAAVFANASTR"
     assembly_gap    3201..3210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3457..>4239)
                     /locus_tag="PK198_10760"
     CDS             complement(3457..>4239)
                     /locus_tag="PK198_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39262.1"
                     /translation="LRFKLRHSMLPIEKLPEPQDLLDYKRESSAPTYDGFTAFRQERN
                     DAGNRIPRNETPFYKLREQLLREQKFVCAYCGQKDLQVETQHGKAQMKTEHFLPQNDE
                     AGNDLNYQNLLGCCLGNDNKKGKNHCDSMKGDKVLQQITNPAAMQCRDEDIIYKVNNA
                     SEEVQLDTSNDNKRKELIGDTKGCLNLNHDHLRKERFSIFKNVLKRRLGEDETQWNFQ
                     EVQRLISDYDDSSDGHHKAFKDFVTWYLRDWLVKNQSNTSRR"
     assembly_gap    4240..4253
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4254..>5791)
                     /locus_tag="PK198_10765"
     CDS             complement(<4254..>5791)
                     /locus_tag="PK198_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39263.1"
                     /translation="ETAAALGGFSKVGQKALWEKQYEGTDYIKNLELFLTEDRNRIDA
                     VLNIQRRSGYSLAFDHALNRLNETYFLEQLKVIQQYRQAASEALPEPPAPRIKTLNIQ
                     QLTLNNVGLFEQCSVQFGRRATCFIGENGGGKTTLLRAVALGLVGTGSPLIDTENELQ
                     NLPRIIGVDESYLFRYAGDGAIQVSYDLNGSIFENGKANLIPFKTLPDTGIVEFGGDR
                     IEPNGFGLPVGEDGTDGDGELPILVIGYPQRYGRKPDGTDIKKRSPNPNAYDIIPLIH
                     DAEDKRIDSLKLWISETWNQSDAHKLRVKALFRIISEVLTQTDGEAFVLELKNAVSPR
                     KIAVTTPFNPEGIPFDLLSTGLSNLFGWIGHLISRMYEAYRFSENPTQESAIVLVDEV
                     DNYLHPAVQARAIPVLLSEFPNVQFVFTSHSPVVLATLQNEGVKAYRVEKGEIFEIDH
                     YYGRTIQDLLLEEYGINKRPATEIQYKIDEMFRAIALNQKEAAKAIFEQLLPILGASD
                     AAIQ"
     assembly_gap    5792..5883
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5884..6084)
                     /locus_tag="PK198_10770"
     CDS             complement(<5884..6084)
                     /locus_tag="PK198_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39264.1"
                     /translation="MEDIRGIHERVLLFLLEWRSKYDNALVFTLRRRPIERLQSGMWF
                     LGNEYYLAFSFWTGTDWVNKTQN"
     gene            complement(6251..>7852)
                     /locus_tag="PK198_10775"
     CDS             complement(6251..>7852)
                     /locus_tag="PK198_10775"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF39265.1"
                     /translation="ESSTRMNGLFANGRVGLDYFISNRSTLTFSGMLMRGKFQPTDAL
                     TTTTDLLFPGNTLTSSYLRSSQQDRNFRNLGTSLQFKHLFPEKGAEWTADLNYNRVRF
                     EGGSEFMTTFDTGAATREKQEGMGQGSFITLQTDFINPLSPSTKIEGGIKATLRNNRN
                     DNANFVFSDTENNWQQIAQISDHFKFNDNVYAAYIQGSHQRGNWGFQAGLRAESSFYT
                     GALTDRDSSFTIAYPISLFPSLFLTRKLNDGQMQLAYTRRVNRPNFFQTMPFTDFSDS
                     LNLRRGNVQLLPEFTNSLELSYQKIFNKGHNLLVSLYYKQASNLITSYQFTEYNADLG
                     KEVVVTSFANGNNAAAYGAELTMKNSFSNWLNLTHNINVYQQQVDATNVESSLELSRL
                     SAFLKETVQIKLPAGFSLQFNGEYRTRASFTPVTNNDPFSGGGPRGQNTAQGYTKAYW
                     FVDASVRKDLMKNQASLTLSMQDVFASRRMGSYTATDFFTQDNFRLMNPQMVRLNFAY
                     RFGKMDASLFTRKNNRVNMQGSDMM"
     assembly_gap    7853..7930
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7931..>8179)
                     /locus_tag="PK198_10780"
     CDS             complement(<7931..>8179)
                     /locus_tag="PK198_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39266.1"
                     /translation="TLSLDQIPAADIESVEVITNPSSKFDAGGGAAGIVNIVLKKEKR
                     MGYNGNVRVGGDSRGGLNLGGDINARQGKLNFFASGMF"
     assembly_gap    8180..8274
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8275..8884)
                     /locus_tag="PK198_10785"
     CDS             complement(<8275..8884)
                     /locus_tag="PK198_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39267.1"
                     /translation="MKSTMQKSFFFTALLAISTLLPLAAQGPQGGNFDPSKMNIGRMY
                     GRVVDEAGKGVGYATVQLFGKKFDPQTQTLRDTTWAGQLSEDNGDFNLEKLPVVGEFE
                     LVISFLGYTEVRQKVDFGIKAPAASLGGGKPSGPSPGGGFPGAGMGSFEKDLGNIKLL
                     EGATVLSEVVVKGQVATTTLALDRKAYRVDKDLSAAGGTAQDA"
     gene            9034..10077
                     /locus_tag="PK198_10790"
     CDS             9034..10077
                     /locus_tag="PK198_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF39268.1"
                     /translation="MKKSLPILLQIAVWVAIWSVFAFTGNTSEHLPGYYMVITVRVLV
                     LALFYNLAYYLLLPLYFSDKKRLFYLLLPLFFVGYVGFSVGVDLTLAKPEKMFANAEK
                     RGHSSKNRSWSWLLIPPVFFSTAIFGAAAGFRGVLEFENKKMAEEEANRRRLEAEIAL
                     LKSQINPHFLLNTLNNLYGIAVTEPDKAPEALLKLSDMVRYILYDCAQPVVPISQELD
                     FIKNYISLQQLRLPPNVSLRVDIPERDPGGAIEPMILIPFVENAFKHGLTTRKPCEIV
                     VVVGAENNRLNLRVENEVFAHQENRQGNESGIGLANVMQRLEHAYAGQYKLNIAETNG
                     RHSVFLDLQLGAA"
     gene            10074..10775
                     /locus_tag="PK198_10795"
     CDS             10074..10775
                     /locus_tag="PK198_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF39269.1"
                     /translation="MTFIALDDEPIALAIIERYARNVPDLQLVATFTDAAAAAEFLRN
                     NTVDLLLSDINMPDVSGLQFVRYLPDERPMVIFVTAYKEHAHEGFDLNVIDYLVKPVS
                     PERFNTAIQKAAGLLELRRKAEAAEASALPDEFFFVFSEYQQVKITISDLLYVEAMGD
                     YVKLFLSTQPKPVLTLERMKILADRLQQHGFRRIHRSYLVNMKKVAALQKARLRVADV
                     WLPVGETYMEGLKDL"
BASE COUNT         2535 a         2579 c         2714 g         2724 t
ORIGIN      
        1 cctgaccgac gacgcgcaca agaacatgca gttcgactac aatttcctca atctggcgct
       61 gaatatcact acaacacagg gcacactgca ctttttgtac gatgccaccg gaagaaagtg
      121 ggccaagagt ggccccgacg gcatccgctt ctacgcagga aacatcgagt acttggatgg
      181 aaaaatacag cacatcgcct tcccggacgg cagacttgtg gcggaataca acacatccgg
      241 cacgagcgta acaggctata gatcagaata cttccgcacg gaccacctcg gcaatacccg
      301 cctgactttc tcggatttca atcaggacgg cctcatctcc tggaaactgc ccaccgcagg
      361 gcctgccgca caggagagcg agatcacatc cgaaaatcat tactacccgt ttgggctgag
      421 ccagaagggg ccttggtatg cgagcgtggc gccggagaat aagtaccgct acaacggcaa
      481 ggagtggaat gatgagtggg ggcttggcct gtatgacttc ggcaaccgct ggcatgaccc
      541 gacgctggga cggtttacta cggtggacag gtttgcggag aagtatgcct tccagtcggc
      601 ttatgtgatg gccgccaatg acccgatgaa atttgtggat gtgaatgggg atagtatttt
      661 tatacgaaca ggcaagggaa aagaaaatat tgcgctatat gacaatgggg tggttacaaa
      721 tagagatggt actaaatata caggaaaggg tgttaaagaa agaaaagacg gctctgtgaa
      781 attacaagga ttcttgaagc aaacagtcaa cgctttagat aaaatacgat ctggagggcc
      841 agtcggaagt aatttagttt caactttaga aaaagataat gacatattcg ttatcactaa
      901 aggacccgtt aacgatgcaa gagggagagc agttcacttc aatcctgcaa gctttgaggc
      961 gggtttagat gttaatggat cgagaaacac atctccattt gtctcgttag cacatgaatt
     1021 agcccacgct ctagattggg ataacggatc aattgatacc agaacatgga tcacttacag
     1081 tgataataag actacgtcaa ctcgtgcaga gatatatgcc agccatgtag agaaccaaat
     1141 tagagccgaa aacaatctcc cattaaggga attttatggt attgatcaag gcaaaggagt
     1201 tggtcggctg ttaatacccg gtactcgaat gagcgcccat tacatgcaaa cgcagaaggt
     1261 tccaacgctt attcaaatac cttttaaata ctaacaatat gaaatctgtt tttttatgtg
     1321 tattcttgct gatgcaggga ggtatttatt ctcaaatcgg ttctgttatt gaatttatcg
     1381 acccttcgct ttttaaaggt aaaggtgtga tatttgtaga gaatttatct cccccgatcc
     1441 aattgccgaa aaatgcaaaa ataataaatc tcactaaaga acaggtgtat atttcagaat
     1501 tgattttttt aaatcaattt tcccaaaatg caactctcgc attttttaac aacaagaata
     1561 taaagaaata tttttggtat tataataggc aatacttggg ttttaaagat gagaatgggc
     1621 gtattaatgt catcattaat ttacttaatt ttcgaaataa aaaaatagct gagaagaaat
     1681 ttgagggttg gggagaagaa tattttattg gcttcggtga atattatgag aaaaatacgc
     1741 taagaattag cgtcaacatt gatttgtgca aagttgacgt atggtaaccg ctggcacagg
     1801 gtcttctctc atcccgaaaa aatttaaaaa aaacaatcgc tggtagcggt ggacgcttta
     1861 attcgttgtc tctgacaaca tactgaaaaa caaattttta catcaaattt ggccaccgac
     1921 tggcgtagag tcttccgaaa aaaataaaaa aacacacaat cgctggtagc acggtctctg
     1981 cccggttatc cttatttcgt tgtctctccg acaacaccca ttactatctg cccttgccct
     2041 cctcatctat aaatccgctg aacatactac cttcgcagcc aaacagtgat ctcacgccat
     2101 gatcgaacaa cttcgcgcta cccacgccgc tgcggcacag attcgccgcc tgtcggatgc
     2161 cgccaaaacc aagctgctgc tgcaattggc ccaagccgta gaagccgcct gcgatgccat
     2221 tctggccgcc aatcagctcg atttggccaa aatagacgac gccgatccta aaaaagaccg
     2281 cctgctcctg aacccggaac gcatcgccgc gctggccggc agcatccggg aagtggccgc
     2341 cctgcgcgat ccctgcggcg cggtgctctt ggataaaatt ttgcccaatg gcctgaaatt
     2401 gagaaaaata agcgtgccgc tgggcgtggt gggcatcatt tacgaatcgc ggcccaacgt
     2461 cactttggat gtgagtgcgc tgtgcctgcg ttcgggcaat gccgctgtgt tacggggtgg
     2521 aagcgacgcc ctgcaaacca atcgcgccct cgcggccatc attcaggatg tgctggagca
     2581 ggccggtctc agccgcgacg tggtggccct cttgccgccc gaccgggaat tggtaaaaga
     2641 catgctgaca gccacgcgct acatagatgt gatcataccg agaggctcgg atgcgctgat
     2701 tcggtttgtg cgcgaaaact cattggtacc cgtcatcgaa accggggccg gcgtgtgcca
     2761 tacttatgta cacgaaagtg ccgatctgga gaaggccaaa gccattgtgg tgaatgccaa
     2821 agtgtcgcgg ccgtcggtgt gcaactcttt ggataccatt gtaatagact caaaaattgc
     2881 gcctgctttt ttgccgctgc tgcgggatga ttttgaaaaa tggaaggtgg aggtttttgc
     2941 cgatccggtg gcgttcgaca ttttacaaca agccggtttt gagcggttgc agccagcctc
     3001 ggaatcggat ttcgggcggg agtttttgga ttacaaatgc tcggtgaaaa cggtgaacgg
     3061 cctgccggaa gcgctggacc acattcgcac ctattcgtcg cggcactcgg aggccatcgt
     3121 ggcggaagac acggcggtgt gtcgcacctt tttggaagaa accgatgcgg cggcggtgtt
     3181 tgccaatgct tccacgcgtt nnnnnnnnnn accgacgtgg gcatgttcgg cctcggtgcg
     3241 gaaatcggta tttctaccca gaaactgcac gctcgcgggc cgtttgcatt ggagaaattg
     3301 gtgacggaaa agtgggtggt ggagggcgaa gggcaggtgc ggtggtgaga ggcagtgtga
     3361 tgtggaacgg tttcaaatgt tgcaggagag cggggggatt gtgttttggt gagtaaagac
     3421 ggaagggggt cggcttttta taccctgctt ggcactttag cggcgcgagg tattggattg
     3481 gtttttcacc agccaatccc tcaagtacca cgttacaaag tctttgaatg ccttgtgatg
     3541 gccatctgaa gaatcatcat aatccgaaat caggcgctga acttcctgaa aattccattg
     3601 ggtttcgtct tcccccagcc gccttttgag tacattttta aagattgaaa accgctcttt
     3661 tctcagatga tcatggttca gatttaaaca gcctttagta tcgccgatca actctttcct
     3721 tttgttgtca ttggaggtat ctaactgcac ttcttcggag gcattattta ccttataaat
     3781 gatgtcttca tctctgcatt gcatagcggc aggattggtg atttgctgca acaccttatc
     3841 ccctttcata gagtcgcaat gattttttcc tttcttgttg tcattgccaa ggcagcagcc
     3901 gagcaggttt tgatagttta aatcatttcc cgcttcgtcg ttttgtggca gaaaatgctc
     3961 agttttcatt tgggctttac cgtgctgagt ttcaacttgt aagtcttttt gcccgcaata
     4021 agcacagacg aatttttgtt cgcggagcaa ttgttctctc agtttgtaaa agggcgtttc
     4081 gttccgtgga attcgatttc ctgcatcatt gcgttcctgc ctgaatgccg tgaagccgtc
     4141 atacgtcggc gcactcgatt cacgcttata atcgagcagg tcttgcggtt ccggtagttt
     4201 ttcaatgggg agcatggagt gtcttaattt aaatcgtaan nnnnnnnnnn nnntcttgaa
     4261 tggcagcatc agaagctcca agtatgggga gcaactgctc aaaaattgct tttgcagctt
     4321 ctttttgatt caacgcgatg gcacgaaaca tttcatcaat tttgtattga atttcagtcg
     4381 ccggccgctt gttgatacca tattcttcca acaataaatc ctgaatagtg cgaccgtaat
     4441 aatgatctat ttcaaaaatt tcgcctttct cgacccgata agcctttact ccttcatttt
     4501 gaagtgttgc caagaccacc ggagaatgtg atgtaaaaac gaattggaca ttgggaaact
     4561 cggataaaag caccggaatt gcgcgcgcct gtactgccgg gtgcagatag ttatccactt
     4621 catccactaa aacgatggca gattcctgag tcggattttc tgaaaaacgg tatgcttcat
     4681 acattctgct gatcaagtgg ccaatccatc cgaaaaggtt actcaggccg gtacttagca
     4741 aatcaaacgg aattccttcc gggttgaaag gagttgttac ggcgatttta cgtggcgaaa
     4801 cagcattttt caattcaagc acaaatgcct ccccgtctgt ttgggtcaaa acctcagaaa
     4861 taattcggaa caatgctttt accctcaatt tgtgtgcatc actttggttc caggtttcac
     4921 tgatccaaag tttcagacta tcaattcgtt tgtcctcggc atcatgtatc aatggaataa
     4981 tatcgtaagc gttagggttg ggagaacgtt ttttaatgtc tgttccatcc ggttttctac
     5041 cgtaccgttg tgggtaacca attactaaaa tgggcagttc gccgtctccg tcagtaccat
     5101 cctccccaac aggcagcccg aagccgttcg gttctattct gtctccgcca aactccacaa
     5161 tgccggtatc aggtaaagtt ttaaacggaa tcaaattggc tttaccattt tcaaatatac
     5221 ttccgttgag atcgtatgac acctgaatgg ctccatcgcc tgcatatcgg aatagatagc
     5281 tctcatccac acctattatg cgcggaagat tttgcaactc attttcggta tcaatcaaag
     5341 ggcttccggt acctaccagc cctaatgcta cggcacgcag aagcgtggtc tttcctccgc
     5401 cgttttcacc tataaaacaa gtcgctctcc tgccaaattg cacactacat tgctcaaaca
     5461 atccgacatt attgagggtc agttgttgga tgtttaaagt ttttatacgc ggagcaggcg
     5521 gttcgggcag agcctctgag gctgcctggc gatattgttg aataacttta agctgctcaa
     5581 gaaaataagt ttcatttagt ctgttcaatg cgtggtcaaa tgccaaactg taaccgctgc
     5641 gcctttggat attcaaaacg gcatcaatcc tgtttctgtc ttccgtcaaa aacaattcca
     5701 gatttttgat ataatccgtt ccttcatatt gtttttccca caatgccttt tgtccaactt
     5761 ttgaaaaacc tcctaacgcg gcagcagttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnattttga gtcttgttga cccagtcggt tcctgtccag aatgaaaaag ccagataata
     5941 ctcatttccc agaaaccaca tgccggattg cagcctctca atgggacgcc ggcgaagcgt
     6001 aaataccaaa gcgttgtcat atttagaccg ccattctaat aaaaacagga gtacccgttc
     6061 atgtatccct ctaatgtctt ccatatcaat tcattttcgg tacaaagata atgatataat
     6121 gctgtacgct ctatacccgg ccccatcctc catatttgcc cctgaaaaag gacgttccta
     6181 actattacaa aaaaagccgc ccccaaaagc tgagagcgac tccttttaaa ccagtctaat
     6241 atattgattc ttacatcata tcgctgccct gcatattgac ccggttgttt ttgcgggtga
     6301 acagcgaggc gtccattttg ccgaaacggt aggcaaagtt gaggcgtacc atttgggggt
     6361 tcatcaggcg gaaattatcc tgcgtgaaga agtcggtagc ggtgtaagac cccattcttc
     6421 tcgatgcaaa cacgtcctgc atactgagtg tgagcgatgc ctgatttttc atcaaatctt
     6481 tgcgcaccga ggcatccaca aaccagtacg ctttggtgta accctgggcc gtgttttgac
     6541 cgcgggggcc gccgccgctg aacggatcgt tgttggtcac gggcgtaaac gaggcgcggg
     6601 tgcggtattc gccattgaac tggagtgaaa atccggcggg caatttgatc tgcacggttt
     6661 ctttcaggaa ggcgctcaaa cggctcagtt ccagcgagct ttccacgttg gtggcgtcca
     6721 cctgctgttg atacacgttg atgttgtgcg tcagattgag ccagttggag aagctgtttt
     6781 tcatagtgag ttcggcgcca taagcggcgg cgttgttgcc gttggcaaag ctcgtcacca
     6841 ccacctcttt gccgaggtcc gcgttgtact cggtaaactg gtaggaagta atcaggttgg
     6901 aggcctgttt gtaatacaag gacacgagga gattgtggcc tttattgaaa atcttttggt
     6961 acgaaagctc cagcgaattg gtgaactccg gcagcaattg cacgttgccg cgacgcaggt
     7021 tgagcgagtc ggagaagtcg gtgaacggca tggtctggaa gaagttgggg cggttcacac
     7081 ggcgggtgta ggcgagctgc atctggccat cgttgagttt gcgggtgagg aacaggctgg
     7141 gaaacaggct gatgggatac gcaatagtga aagacgagtc gcggtcggtg agtgcgccgg
     7201 tgtagaaact gctctctgcg cggaggccgg cctgaaagcc ccagttgccg cgctgatggc
     7261 tgccctgaat gtaagcggca taaacgttgt cgttgaattt gaagtggtcg gaaatctgag
     7321 caatttgctg ccagttgttt tcggtatcgc tgaatacgaa gttggcgttg tcgttgcgat
     7381 tgttgcgcag ggttgccttg atgccgcctt caattttggt gcttgggctg agcgggttga
     7441 tgaagtccgt ttgcagggta atgaagctgc cctggcccat gccttcctgc ttttcgcggg
     7501 tggcggcgcc ggtgtcgaag gtggtcataa actcgctgcc gccctcaaac ctgacgcgat
     7561 tgtagttcag gtcggcggtc cattcggcgc ctttttccgg aaagaggtgt ttgaactgca
     7621 aagaagtacc gagattgcgg aagttgcggt cttgttggct gctgcgcaag tagctgcttg
     7681 tgagggtgtt gccggggaag agcaggtcgg tggtggtggt aagggcgtcg gtgggttgga
     7741 atttgccgcg catcagcatg ccgctgaaag tgagggtgct gcggttgctg atgaaataat
     7801 ccaggcccac gcggccattg gcaaagaggc cgttcatgcg ggtgctcgat tcnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn ttgaacatgc cggaggcgaa gaaattgagt ttgccttgcc gggcattgat
     7981 gtcgccgccg aggttgaggc cgccgcgcga gtcgccgccc acgcgcacat tgccgttgta
     8041 tcccatgcgc ttttcttttt tcaaaacgat gttgacgatg cccgccgcgc cgccgccggc
     8101 gtcaaatttg gacgaggggt tggtgatgac ctctacgctt tcgatgtctg ccgccggtat
     8161 ttggtcgagc gagagtgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggcgt
     8281 cctgagccgt gccgcctgct gcgctcaggt ctttatccac gcggtaggct ttgcggtcca
     8341 atgcgagcgt ggtggtcgcc acttgccctt ttaccaccac ttcgctgagt acggtggcgc
     8401 cttccagcaa tttgatattg ccgaggtctt tttcaaaact gcccatacct gcgccgggga
     8461 aaccaccgcc gggcgaggga ccggagggct tgccgccgcc gagagacgct gcgggcgctt
     8521 tgatgccgaa gtccactttt tggcgcactt cggtgtagcc gaggaaggaa atgaccaact
     8581 caaactcgcc cacaacaggc agtttttcga ggttgaaatc gccgttgtcc tcgctgagct
     8641 gacctgccca ggtggtgtcg cggagggtct gggtctgagg atcgaatttt ttaccgaaca
     8701 actgcacagt ggcatagccg acgcccttgc cggcttcgtc aactacccgg ccgtacatgc
     8761 gtccgatgtt cattttggag ggatcgaaat tgccgccctg cggcccctgt gccgccaatg
     8821 gaagcagcgt ggatatggcc aataatgccg tgaagaaaaa tgatttttgc atggtgcttt
     8881 tcatgtttgt cgggtgattt cttttgaatg acggtgcaaa gttggcctca aaaaaaataa
     8941 aaaatggtgt gtgccgacga atcggccaaa tgtatggatg aaccgcccgg cttgctcagg
     9001 cgcggcatcg aaaagattgc gaactttgca ccgatgaaaa aatcgcttcc catcttacta
     9061 caaatcgccg tctgggtcgc catctggtcg gtcttcgcct tcaccggcaa taccagcgaa
     9121 cacctgccgg gatattacat ggtcatcacg gtgcgcgtgt tggtgcttgc gctgttttac
     9181 aacctcgcct actacctgct gctgccgctg tatttttcag acaaaaaacg cctgttttac
     9241 ctcctcttgc cgctgttctt tgtgggatat gtggggtttt cggtcggcgt tgatctgact
     9301 ttggccaagc cggaaaagat gtttgccaac gcagaaaaac gagggcattc ctccaaaaat
     9361 cgctcctggt cctggctgct cattccgcct gtttttttca gcacggctat tttcggggct
     9421 gctgccggat ttcggggggt actggaattt gaaaataaaa aaatggccga ggaggaagcc
     9481 aaccgccgcc gcctcgaagc ggaaatcgcg ctgctcaaat cgcagatcaa cccgcatttc
     9541 ctgctcaata cgctcaataa cctctacggc attgccgtca ccgagccgga caaggcgccg
     9601 gaagccctgc tcaaactctc ggacatggtg cgctatattc tctacgattg cgcccagcct
     9661 gtggtgccga tctcgcagga actcgatttt atcaaaaatt acatctcgct gcaacaactc
     9721 cggctgccgc ccaatgtctc ccttcgcgtg gacattcccg aacgcgaccc cggcggcgct
     9781 atcgaaccga tgatcctcat tccttttgtg gaaaatgcct tcaaacacgg tcttaccacc
     9841 cggaagccct gcgaaattgt ggtggttgta ggcgccgaaa ataaccggct gaatttgcgg
     9901 gtagagaatg aggtttttgc gcatcaggaa aaccggcagg gcaatgaatc gggcatcggg
     9961 ctggccaatg tgatgcaacg tctggagcac gcctacgccg gccaatacaa gctgaacatc
    10021 gccgaaacaa acggccggca cagcgttttt ctcgatctgc aactcggcgc cgcatgactt
    10081 tcatcgccct cgacgacgaa cccatcgcat tggccatcat cgagcggtat gctcgcaatg
    10141 tgcctgattt gcaattggtt gccactttta cagatgccgc cgctgccgct gaatttctgc
    10201 gcaacaacac cgttgacctg ctcctcagcg acatcaatat gcccgatgtg agcggcctcc
    10261 agtttgtgcg ctatctgccc gacgagcgcc cgatggtcat tttcgtcacg gcctacaaag
    10321 agcatgccca cgagggattc gacctcaatg tgatagatta tttggtgaag cccgtttcgc
    10381 cggagcggtt caatacggcc attcagaaag ccgccggcct gctcgaactg cgccgcaaag
    10441 ccgaagctgc cgaggcgagc gcgctccccg atgagttttt ctttgttttt tcggaatacc
    10501 agcaggttaa aatcaccatc tccgacctgc tttatgtaga ggccatgggc gactatgtga
    10561 agctgttcct cagtacgcag cccaagcctg tactcacgct ggaacgcatg aaaatattag
    10621 ccgaccgcct gcaacagcac ggttttcggc gcatacaccg ctcttatttg gtgaacatga
    10681 aaaaggtggc ggccctccaa aaagcaaggc tgcgggtggc cgatgtctgg ctgccggtgg
    10741 gcgaaactta catggagggg ctgaaggacc tgtaattcat cacacctcca cccattcaaa
    10801 cagccgctgc tccgacttct ttttgtgcag atattccgag g
//
LOCUS       DAORLF010000114        10766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3287_length_10766_cov_2.277472, whole genome shotgun sequence.
ACCESSION   DAORLF010000114 DAORLF010000000
VERSION     DAORLF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_10766_cov_2.277472"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1760
                     /gene="asnS"
                     /locus_tag="PK198_10800"
     CDS             312..1760
                     /gene="asnS"
                     /locus_tag="PK198_10800"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRF39270.1"
                     /translation="MKKRTEISAILQSPEMDSTVTVMGWVRAFRNNRFIALNDGSTPN
                     NLQIVIDAEQLPEDTVRKIGFHACISATGKLAPSLGGGQAVEVLAESVEILGECNLET
                     YPLQPKRQTMEFLRSKAHFRPRTNTFSSVFRIRHAVAFAIHKYYNDRGFYYLHSHIIT
                     GSDAEGAGEMFRVTTLDLEHLPRTESGDIDWKEDFFGRSTNLTVSGQLQAEIGALALG
                     KVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIYDNMDLAEDFCKYLIRYVLDNYY
                     DDVAALDARLAEEEKSLPADKRRPMGLIDQLRFVVDNDFERITYTDAVRILQNSNPYK
                     KGKFEFPVEWGKDLQAEHERYLVEKHFQKPVIVRNYPKAIKAFYMRANDQQPDEPGLT
                     VAAMDVLFPGIGEVIGGSQREERHDALLGRIAEVGIPEEELWWYLELRKFGGAPHAGF
                     GMGFERMVQFITGMSNIRDVIPFPRTPKSAEF"
     gene            1872..2258
                     /locus_tag="PK198_10805"
     CDS             1872..2258
                     /locus_tag="PK198_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRF39271.1"
                     /translation="MIERLNQFTKAFSENAFWKKITRYARQAGLKTVYTALLLYFAYK
                     RPETPTWAKNIALGALGYLISPLDWLPDLTPIFGYTDDIGVLGFGLIMIAGHVNDGVK
                     EKARQQLQRWFGEYEETDLKEVDERI"
     gene            complement(2519..3430)
                     /locus_tag="PK198_10810"
     CDS             complement(2519..3430)
                     /locus_tag="PK198_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HRF39272.1"
                     /translation="MYIHERNNWTNFVWDNDKVTPALSSVRHLQGRLLGRMESLGFDF
                     IERATLESLILDVVDTSRIEGEFLNPELVRSSIARKLGIENAEFVKTPRNIEGIVDVV
                     LDATQNFEEPLTKERLLGWHNSLFQTGYSGYQKIDVAQYRSGGMKVISGNFGREKIHF
                     EAPAAERVPTEMERFLDWLNHKNSLDLVLKSMIAHFWFVTIHPFDDGNGRIARAILDM
                     FLARSDNSKIRFYSLSNQIFKEHRRYNDIIEKTQKGTQDITLYLEWFLACFERALSAS
                     EQNLETILDKAHFWDKHKSTNINDRQR"
     gene            complement(3507..4586)
                     /locus_tag="PK198_10815"
     CDS             complement(3507..4586)
                     /locus_tag="PK198_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRF39273.1"
                     /translation="MSVITAQSETFLRKYLNNASPTGFEAPGQQLWLEYVKPYIHEWS
                     IDNYGTVYGVINPGKDFRVVIEGHADEISWFVNYISDDGFINVIRNGGSDHLIAPSKR
                     VNIHTPKGIVKGVFGWPAIHTRQGDDANLAPKIENIFIDVGAKDKEEVLGMGIHVGCV
                     ITYEDEMIVLNDRYYVGRALDNRMGGFCIAEVARLLHENKIELPYTLYVVNSVQEEVG
                     LHGAEMIAQTIKPNVAIVTDVTHDTHTPLLSAKKLGDVRAGAGPSVTYAPAVHNKLLQ
                     LIIAAAEEKNIPIQREAASRRTGTDTDAFAYSNGGVPSALISLPLRYMHTTVEMAHKD
                     DVNNVIQLIYESLLKIEAGHNFKYF"
     gene            4779..5576
                     /locus_tag="PK198_10820"
     CDS             4779..5576
                     /locus_tag="PK198_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39274.1"
                     /translation="MRYLLPLCFLMFAWTSAAQEPGVPKRAVRLQIGVGAGWETYRDL
                     AMSPLLYEGIQGAAGIGIEVEGARVLHRMEGLFFFGNTTAQRIGGTTENLAFMFSGSH
                     LRRLSPVDTKWQWHAGPALSSWGSLRYHRSLVNSNYFYDLFISLGATGSVERELRFLR
                     RNWRAGWQLSVPVLSWGLRPSYSGLVAATPDEEETPAPYLEEARVGAFGVLTRVNSRL
                     HLTYALPHANHLSLVYHWDMFRSDAGRHPISHAMQALQLNLSVKLSR"
     gene            5573..6577
                     /locus_tag="PK198_10825"
     CDS             5573..6577
                     /locus_tag="PK198_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF39275.1"
                     /translation="MKNKIILFGALLALTFSSCEKLLFDADPADDPVSNFDALWKEVD
                     EGYSFFAYKNINWDSVYSVYRPRVNANTKTLELFNVMSDMLYTLQDGHVNLETGFNRS
                     RNWEWYLAHPDNFDANLLERSYLKGEQWYTGPLIHTIIDTIGYLRYGSFAQRFSESQL
                     DLVIARFSSLKGVVIDVRSNGGGFGSMADLLASRFADRERTAYTFVVKNGPGRNDFSD
                     PVEVKIKPEGERRFTKPVIILTNRRSYSATTFFVAQMSALPHVRIMGDWTGGGAGTPV
                     GTELPNGWTVRYSSTQTFLPDGFNIEGGIPPDIRIDMNEADRQQGKDTILERALAELR
                     "
     gene            6707..7348
                     /locus_tag="PK198_10830"
     CDS             6707..7348
                     /locus_tag="PK198_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39276.1"
                     /translation="MLILPDYLQPMSHLWKYIQSLFKNNTDGERPRHVAQPSQPVHEL
                     IVRSPEELAAMEQWKQTLVCRKLCEWIGLQFALYGSLPNEIDETLDFLDTASSKGFVV
                     HLHKTNYTRQEAIAFFDMLKERVLAQGYRTQISDRRIYRVHSWIETTERHYLKPRPEF
                     GAPGKIRQRFGNITILLILRNDKPWQLKFQATTYRDHLFLEADNFADLMEELV"
     gene            complement(7421..8671)
                     /locus_tag="PK198_10835"
     CDS             complement(7421..8671)
                     /locus_tag="PK198_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39277.1"
                     /translation="MKDLFEKFKDNRGPLGQYSQVADGYFAFPALEGEIGSRMKFRGK
                     EVIVWSLNNYLGLANHPEVRKADAEGAKAWGLAHPMGARIMSGQTSLQIQLEKELAAF
                     VQKEAALLINFGFQGVVSIIDALLDRHDVVVYDKDSHACIYDGVRLHLGKRLPFEHND
                     IESFEKQIKKARLLAEQSGGGVLVITEGVFGMRGEQGILKEIAAYKEEYGFRLFVDDA
                     HGFGALGATGAGAGEEQGVQDQIDLYFSTFAKSMASIGAFVAGDADMMEYLRYNMRSQ
                     LFAKSLPTPLVVGNLKRLELLRSMPELREKLWSNVSKLRNGLRERGFDVGAANSCVTP
                     VFMHGSPFEAGNLVLDMRENYNIFCSVVMYPMIPKGMILLRLIPTAAHTDADIEETLH
                     AFGEVARKLKAGEYEHALDLHLIS"
     gene            complement(8726..9166)
                     /locus_tag="PK198_10840"
     CDS             complement(8726..9166)
                     /locus_tag="PK198_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF39278.1"
                     /translation="MNPEFDKKVSQTIQFSVPVMSVEELKSNTQKLRIFDARERAEFE
                     VSHILGAEWVGYRHFDIRAYAKLPKETPIVVYCSIGYRSEKIGEQLKANGFTEVYNLY
                     GSIFEWANKGYPLVDGKGREVREVHTYNKEWSRWVDETKAKKIW"
     gene            complement(9269..>9908)
                     /locus_tag="PK198_10845"
     CDS             complement(9269..>9908)
                     /locus_tag="PK198_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRF39279.1"
                     /translation="NNQEVFYQKASYIARLGSGSACRSVYPKLAVWGETGAIPEASNL
                     YAIPYTAAHSVFQSFHDDILLISQKEKSVSSRAGHGLMEGNIYADNRYTQAKQRTTEL
                     NNILQDGDIEAFGRIAESEAMTLHALMMASNPPYILLQPNTITAIEKVRDFRNTKKTP
                     LYFSLDAGPNLHLLYPDSAKDTVKKFIREELAPLCQNNQWIEDEAGEGPEPL"
     assembly_gap    9909..9920
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9921..10260)
                     /locus_tag="PK198_10850"
     CDS             complement(<9921..10260)
                     /locus_tag="PK198_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRF39280.1"
                     /translation="MAIIKYWGKYGTQLPRNPSISFTLKNAYTETWLEYKPKTGNYNQ
                     ISLNLFFQNQPNEAFRSKVLGFMESIIDVFPFLRQLEWTIQTRNSFPHSAGIASSASA
                     MSALALCLCSM"
     gene            complement(10376..>10766)
                     /locus_tag="PK198_10855"
     CDS             complement(10376..>10766)
                     /locus_tag="PK198_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRF39281.1"
                     /translation="FARMLDFFSKENCSTWNNMTASISGYAYNDSQTRKAVQEVYSKH
                     NYIIDPHGAVAYLALGEYQQKHPDTIGVLLETAHPAKFLNDMNDILNQSIDIPERLST
                     LANKPKEATLTDTNFDVFKKHLLNQYA"
BASE COUNT         2473 a         2666 c         2757 g         2858 t
ORIGIN      
        1 agaaaatata cctcgcaccg tcaggtgcga taccgggcgc tgctaattaa taacttcttg
       61 tgccgaaagc tatacacaaa acctggcggc gcaaggtaaa catcattgca tactgaggaa
      121 tccgggatgc aaaaaatgtt tgcttggcaa agatagggct ttgttttttt taaatgcaag
      181 aggcaggtgg aaaaaatatg cccaagtttg atgtcccgaa gtttagtaat cggtagttcc
      241 tctttttccg taattttgcg ccttcttttc agaacgcctc ctgcttggag cgcacacaac
      301 cattataaga catgaaaaaa cgcacagaaa tctccgccat cctgcaaagc cctgaaatgg
      361 acagcaccgt caccgtcatg ggctgggtgc gcgcttttcg caataaccga ttcatcgccc
      421 tcaacgatgg ttctacgccc aataacctcc agatcgttat agatgccgag caattgccgg
      481 aagacactgt gcgcaaaata ggctttcatg cctgtatttc agcaacgggc aaattggcgc
      541 cctcactcgg cggcgggcag gcagtggaag tactggccga atcggtggaa attcttggcg
      601 agtgcaacct cgaaacttac cccctgcaac ccaagcgcca aacgatggag ttcctgcgtt
      661 ccaaagcgca tttccgtccg cgcaccaata ccttcagttc tgtattccgc atccgccatg
      721 cggtggcctt tgccattcat aaatattaca acgaccgcgg attttactac ctccacagtc
      781 acatcatcac cggcagcgat gccgaaggcg ccggggagat gttccgcgtg acgaccctcg
      841 acctcgaaca cctgccccgc accgagtcgg gagatattga ctggaaggaa gacttcttcg
      901 gtcgctccac caacctcacg gtatccggtc agttgcaggc cgaaatcggg gcgctggcgc
      961 tgggcaaggt ttatacgttt ggccctacct tccgggccga gaactccaac acgccgcgcc
     1021 acttggccga gttttggatg atagagccgg aaatggcttt ttacgatatt tacgacaata
     1081 tggacctggc ggaagacttc tgcaagtacc tcatccgcta tgtgctcgac aactactacg
     1141 atgatgtggc cgctctggac gcccgcctcg cagaggaaga aaaatcgctg cctgccgaca
     1201 agcgtcgccc gatgggattg attgatcaac tgcgctttgt ggtggacaat gatttcgagc
     1261 gcatcactta cacggatgct gttcgcattt tgcaaaattc caatccttac aaaaaaggca
     1321 agttcgagtt cccggtagaa tggggcaaag accttcaggc cgaacacgaa cgctacttgg
     1381 tggagaagca cttccaaaag ccggtcattg tgcgcaacta ccccaaagcc atcaaagcat
     1441 tttacatgcg cgccaacgat cagcagcccg atgaacccgg cttgactgta gccgctatgg
     1501 atgtactctt tcccggcatc ggcgaggtca tcggcggttc gcagcgcgaa gagcggcatg
     1561 atgcattgct cggccgcatt gcagaagtgg gtattcccga agaggaactc tggtggtacc
     1621 tcgaattgcg caagttcggc ggcgcgccgc acgcaggctt cggcatgggc ttcgagcgca
     1681 tggtgcagtt catcaccggc atgagcaata tccgcgacgt gattcccttc ccccgcacgc
     1741 cgaagagcgc ggagttttga tgtgaggtga taggctgata ttcgcttttc gtcgagtaat
     1801 cgggcatagt ctcaaccttt tgccggcatc gcgccgttat tcgggcattg tttcatcatt
     1861 gagtatatcg catgatcgaa cggctgaatc aatttaccaa ggctttttcc gaaaacgcat
     1921 tttggaaaaa aatcacccgt tatgcccggc aggccggact aaaaacggtt tacactgcct
     1981 tgcttttgta ttttgcttac aagcgccccg aaacgccgac ctgggccaaa aacatagcct
     2041 tgggcgcgct ggggtacctc atatctccat tggattggct gcccgatctg acgcccattt
     2101 tcggctacac cgacgacatc ggcgtgctgg gcttcgggct gattatgatt gccggccatg
     2161 tcaatgacgg tgtcaaagag aaagcacgcc aacaattgca acgctggttt ggcgagtacg
     2221 aggagaccga tttgaaggag gtggatgagc ggatttgagg tgcttgtcgg ggtagtgttt
     2281 gtagtgtgga gctacggact tttcaaggtg caattttcgg tattctcaag cctttcggac
     2341 ttacgatgat gtaatttgtc ttcttaccgc tgtcttctgc tttcagcatt tcttttgaaa
     2401 ctaaatcctg taaatcccgc aatgcagtgt cagttgagca tttcgtcagt ttagcgtaag
     2461 tggatgttcg caaaaatcca gtttcttgag cgtaattgtc gtataaataa ttgattacct
     2521 atcgttgtct gtcattgata ttggttgact tatgcttgtc ccagaagtgt gctttatcca
     2581 aaatggtttc caaattttgc tcacttgcgg acagcgctct ttcaaagcaa gccaaaaacc
     2641 attccaaata cagcgtgatg tcttgtgtgc ctttttgtgt tttttcgatg atgtcattat
     2701 agcgcctgtg ttctttaaaa atttggttgg ataagctgta aaaacgaatt ttactgttat
     2761 cgctcctggc taaaaacata tccaaaatag cgcgggctat tcgtccgttt ccgtcatcaa
     2821 aagggtgaat ggtgacaaac caaaagtgtg cgatcataga cttcagcact aagtccaaac
     2881 tgtttttatg gttgagccag tctaaaaacc gttccatttc ggttggtacc cgctcggctg
     2941 ccggcgcttc aaaatgtatt ttttctctgc cgaaattccc ggaaataact ttcatgccgc
     3001 ccgaacgata ttgagccaca tcaattttct gatagccgct atacccggtt tggaacagtg
     3061 aattgtgcca gcccaaaagt ctttccttag tgagcggttc ttcaaaattt tgcgtagcat
     3121 ctaaaacgac atccacaata ccttcgatgt tgcgaggcgt ttttacaaat tcagcatttt
     3181 cgatgcccaa ttttctggca atagaggagc gaaccaattc gggatttaaa aactctcctt
     3241 caattcttga ggtatcaaca acgtctaata taagcgactc caaagtggct cgttctatga
     3301 aatcgaagcc taaactttcc atacgaccca gcaagcgccc ctgtaaatgc cttacagaag
     3361 acaaggcagg cgttacctta tcgttgtccc aaacaaagtt tgtccaattg tttctttcgt
     3421 gtatgtacat gtttaccgcc gcaaaagttg cggtgaaaat acgctttttt caccgcaaga
     3481 gggggtttgg gctaagtttt atttttttaa aaatacttaa aattatgccc cgcctcaatt
     3541 ttcagcagac tttcataaat caactgaatc acattattta catcgtcctt gtgggccatc
     3601 tccacggtag tgtgcatgta gcgcagcggc agtgaaatga gcgccgaggg tacgccgccg
     3661 ttggaatagg cgaaggcatc cgtatcggtg ccggtgcgac gcgaggccgc ttcccgttgt
     3721 atgggaatgt tcttttcttc ggcagccgct atgatgagtt gcagcagttt gttgtgtacc
     3781 gccggcgcgt acgtcacgct cggtccggcg cccgcccgga cgtcgcccaa ttttttggcg
     3841 ctcagcaagg gcgtgtgcgt gtcgtgcgtc acgtcggtga cgatggccac gttcggtttg
     3901 atggtttgtg caatcatctc ggcgccgtgc agccccactt cctcttgtac cgagttgacg
     3961 acatacaaag tataaggcag ttcgattttg ttttcgtgca gcaggcgggc cacctcggca
     4021 atacagaatc cacccatgcg gttgtccaaa gcgcggccca catagtagcg gtcgttgagt
     4081 acgatcatct cgtcttcata agtgatgacg cagccgacgt ggatgcccat gcccaatact
     4141 tcttccttgt ctttggcgcc cacgtcaatg aagatgtttt caattttcgg ggccagattc
     4201 gcgtcgtcgc cctgccgggt atgaatagca ggccagccga acacgccttt gacgatgcct
     4261 ttcggagtgt ggatgttcac tcgtttggag ggcgcgatga ggtggtcgct gccgccgttg
     4321 cgaatgacgt tgataaaacc gtcgtcggag atgtaattga caaaccagga aatttcatcg
     4381 gcatggcctt cgatgactac ccggaaatcc ttaccgggat tgatgacgcc atacacggtg
     4441 ccgtagttgt ctatgctcca ctcatggatg tagggcttga cgtattccaa ccacagttgc
     4501 tgtccggggg cttcgaagcc ggtgggagaa gcgttgttga gatatttacg gaggaatgtc
     4561 tccgactgag cagtaattac cgacatagta atagtatggt tgtatcaggc acggattggt
     4621 tttgtggtgt taaggcctct tcccccgtgc gtttattcgg gcaaaattag aaatttcctc
     4681 caaacggcgc atgtttttcc gcaagggctt gtatcacctt gttgtatggc agttttgaat
     4741 ccgttagctt tgtcgttatt atcaaaatat ggtatgaaat gcgatatctg cttcctcttt
     4801 gttttttgat gtttgcatgg acgtcggcgg cgcaagaacc cggcgtgcca aagcgcgctg
     4861 tccgtttgca gataggcgtg ggcgccggat gggaaactta ccgggacctc gccatgtcgc
     4921 ccttgttgta tgagggcatt caaggggctg ccggcatagg catcgaggta gagggcgcgc
     4981 gtgtattgca tcggatggag gggctgtttt tctttggcaa taccaccgct caacgcatag
     5041 gcggcactac tgaaaactta gctttcatgt tcagtggcag tcatctccgg cggctcagcc
     5101 ctgtggatac gaagtggcaa tggcatgccg ggccggcgct cagttcctgg ggcagcttgc
     5161 ggtatcaccg gagtttggtc aattccaatt atttctatga cctgttcatt tcgctggggg
     5221 ctaccggatc agtcgagcgg gaattgcggt tcttgcgtcg caactggcgg gctggctggc
     5281 aacttagcgt gccagtcttg agttgggggc ttcggccttc gtattccggt ttggtggcag
     5341 ccacgccgga tgaggaagag actccggcgc cctatttgga agaagcccgg gtgggagcgt
     5401 tcggcgtgct gacccgcgtg aattccagat tacacctgac ttacgccctg ccccatgcca
     5461 accatctgag cttggtttat cactgggata tgtttcgctc ggatgcgggg cgccacccga
     5521 tcagccacgc catgcaggca cttcaattga acctttcggt aaaactatcg agatgaaaaa
     5581 caagatcatt ttattcggcg ctctgctcgc tctcacattt tcttcgtgtg aaaaactctt
     5641 gttcgatgcc gatcctgccg atgatccggt gagtaacttc gatgcgcttt ggaaggaagt
     5701 ggatgaaggc tattcttttt ttgcatacaa aaacatcaat tgggacagcg tctattcggt
     5761 atatcggccg cgagtgaatg ccaataccaa aactttggag ctgttcaatg tgatgtccga
     5821 tatgctctac actctgcaag acgggcacgt caatctggaa acaggcttca atcggtctcg
     5881 caactgggaa tggtatctgg cgcatcccga caatttcgac gccaatctat tggaacgttc
     5941 ctacctcaaa ggcgaacaat ggtacaccgg gccgctcatc cataccatta ttgataccat
     6001 cgggtacttg aggtacggca gttttgcgca gcgtttcagc gagtcgcagc tcgatttggt
     6061 cattgccagg ttcagcagtc tcaaaggtgt cgtcattgat gtgcgcagca atggcggcgg
     6121 ctttggctca atggccgatc tgctggcttc ccgcttcgcc gaccgcgaac gaacggctta
     6181 tacttttgtg gtcaaaaacg ggccgggtcg caatgatttt tccgaccccg tggaagtgaa
     6241 aatcaaacca gaaggcgagc ggcgatttac caaaccagtc attattctca ccaacaggcg
     6301 gagctacagc gccactactt tcttcgttgc ccaaatgtcg gcgctacctc atgtccgcat
     6361 catgggcgac tggaccggcg gcggcgcagg aacgcccgta ggcaccgagc ttcccaatgg
     6421 ctggacggtg cgctactctt ccacgcaaac ttttctgccc gatggattca atatcgaagg
     6481 tggcattcca ccagatattc ggatagacat gaacgaggct gaccggcagc agggcaagga
     6541 tactattctg gagcgggctt tggctgagtt gaggtagagg aagtctggca aataatcaag
     6601 aatcatattc cagagctgga gggtaacctt accctgataa cgaccaacta accttacagg
     6661 ggggggccgg cgccagtctt tgagaacaaa catcttctat ctctgtttgt tgatattacc
     6721 tgattacttg cagcccatgt ctcatctttg gaaatacatt cagtctttgt tcaaaaacaa
     6781 taccgatgga gagcggcctc gccatgtagc gcagccttct caaccggtgc atgaactgat
     6841 tgtccgcagc ccggaggaat tggcagctat ggagcaatgg aagcagactt tggtttgccg
     6901 aaaactctgc gaatggatag gcctgcagtt tgccttgtat ggctcactgc cgaatgagat
     6961 tgacgagacg ctcgattttt tagataccgc ctcttctaag gggttcgtgg tgcatcttca
     7021 taaaaccaac tacacccggc aggaagccat cgcctttttc gatatgctca aagagcgggt
     7081 actggctcag ggctatcgaa cgcagatttc cgaccgccgt atctatcggg ttcattcctg
     7141 gatcgagact acggaacggc attacctgaa gccccgaccg gaattcggcg ctccgggcaa
     7201 aatccgtcag cgctttggaa acatcaccat cttgttgatc ttgcgcaacg acaaaccctg
     7261 gcaactcaaa tttcaggcca ctacttaccg cgatcacctc tttctggagg ccgataattt
     7321 cgcggatttg atggaagagt tagtctgagt ccttaggaca taaaaaaagc cgtctccgag
     7381 gatcgaaaac ggctttattt tattgaaaat caatttctta tcaagaaatc aagtgcaaat
     7441 ccagcgcgtg ttcatactcg ccggccttga gtttgcgggc tacctctccg aaggcgtgca
     7501 gcgtctcctc aatgtccgca tcggtatgtg cagcggtggg aatgaggcgc aataagatca
     7561 tacctttcgg gatcatggga tacatgacta ccgagcagaa gatgttgtag ttctcgcgca
     7621 tgtcgagtac gagattgcct gcctcgaacg gagagccgtg catgaacacc ggcgttacgc
     7681 aggagttcgc agcgcctaca tcgaagccgc gctcgcggag gccattgcgc agcttgctca
     7741 cattgctcca cagtttctcg cggagttccg gcatactgcg cagcaattct aagcgcttga
     7801 ggttgcccac taccaaagga gtcggcagcg atttagcaaa caactgcgag cgcatattgt
     7861 aacgcaggta ctccatcatg tcggcatcgc cggcaacgaa ggcgccaata ctggccattg
     7921 atttggcaaa ggtgctgaag tagaggtcta tctgatcttg tacgccctgc tcctcccctg
     7981 ctccggcacc cgtagcaccc aaagcgccga agccgtgcgc gtcgtccaca aacaggcgga
     8041 atccatactc ttctttgtaa gcggcaattt ccttgaggat accctgttcg ccgcgcatgc
     8101 cgaacacacc ttctgtgatg acgagtacgc cgccgccgga ttgctcggcc agcaggcgag
     8161 cctttttgat ctgcttttcg aagctctcaa tgtcgttgtg ttcaaaaggc aggcgcttac
     8221 cgaggtgcaa acgaaccccg tcgtaaatgc aggcatggct gtctttgtca tacactacca
     8281 catcgtggcg gtccaacaaa gcgtcaatga tggagactac gccctgaaaa ccgaagttga
     8341 tgagcaaggc ggcttccttc tgaacaaagg cggccaattc tttttccaac tgaatttgca
     8401 gcgatgtttg ccccgacata atgcgggcgc ccatcggatg agcaaggccc caggctttgg
     8461 cgccttcggc atcggctttg cgcacttcgg ggtgattggc gaggccaagg tagttgttga
     8521 ggctccatac gatcacctct ttgccgcgaa acttcattcg gcttccgatt tctccttcca
     8581 gcgctgggaa tgcaaagtac ccgtctgcta cctgggagta ttggcccagc gggccgcgat
     8641 tgtctttgaa tttttcaaaa agatccttca tgtcaacaat ttaaagtctg attgtgattg
     8701 aaaaaactgt ctgtatgata ctggcttacc agattttttt tgcttttgtc tcgtccaccc
     8761 agcggctcca ttccttgtta tatgtatgca cttcgcgtac ttcccggcct ttgccatcta
     8821 ccaatggata ccctttgttc gcccactcga agatgctgcc gtacaaatta tagacctcgg
     8881 taaacccatt tgcctttagc tgttcgccga tcttttcact cctgtatccg atagagcaat
     8941 aaacaacaat cggcgtttct ttgggtaact tagcataagc acggatgtca aaatgcctgt
     9001 atcctaccca ttcagcgcca agaatgtgac tcacttcaaa ctcggctcgc tcccgcgcgt
     9061 caaagatacg gagtttctgg gtattggatt tcaattcttc cacactcatc acaggtacgc
     9121 tgaactgaat ggtttggctc acctttttat caaattccgg attcatgcag gctggttttt
     9181 gaagaggttt ctgagccagg gcttccacgc taagcacgag gaagagtaag gtgaaatact
     9241 tggaaaacat cgtgcgatca gttgatgatt aaagcggctc cggcccctcc cccgcttcgt
     9301 cttcgatcca ttgattgttt tggcaaagcg gcgcaagttc ctcgcggatg aattttttta
     9361 cagtgtcttt tgcgctgtcg ggatacaata aatggaggtt agggcctgca tcaaggctga
     9421 agtacaacgg cgtttttttt gtgtttctaa agtcgcgtac cttctcaata gccgtgatgg
     9481 tgttgggttg gagtaggatg tagggcggat tggaggccat catcaaggcg tgcaaggtca
     9541 tggcttccga ctcggcaatg cgtccaaaag cctctatgtc gccatcttga aggatgttat
     9601 tcaattccgt cgttcgctgt ttggcctgtg tgtagcggtt atctgcataa atattacctt
     9661 ccattagccc atgccctgcc cggctggaaa cgcttttttc cttttggctg atgaggagta
     9721 tgtcgtcgtg gaaggattga aaaacagaat gagcggcagt gtatggtatc gcgtaaaggt
     9781 tggaggcttc gggaatggcg ccggtttcgc cccatacggc cagcttagga tataccgacc
     9841 gacaggcact gcctgatccc agacgagcga tgtaagatgc tttttgatag aatacctctt
     9901 ggttatttnn nnnnnnnnnn ccatactaca aaggcacaag gccagcgcgc tcatggctga
     9961 ggcagaagat gcgatacccg ccgagtgcgg gaacgaattg cgtgtttgaa tggtccattc
    10021 taactgccgg aggaaaggaa atacatcaat gatgctttcc atgaagccca atactttgct
    10081 tcggaacgct tcattgggct ggttttggaa gaaaaggttc aggcttattt gattgtaatt
    10141 accggtcttt ggcttgtatt ctaaccacgt ttccgtgtag gcgtttttta gcgtaaagct
    10201 gatggatgga tttctgggta gttgagtgcc atatttgccc cagtatttta tgatggccaa
    10261 attggacggg cttctccagc ctacactacc tggcttggta ttggcggaat ctatgaccaa
    10321 ttttgagttc ttgtagtctg tcatggtgcg tgtttaatgt tccacgtgga acatattagg
    10381 cgtattgatt gagtaggtgt tttttgaaga cgtcaaaatt agtgtctgtg agcgttgctt
    10441 ctttaggttt gttagctaag gtggataatc tttctggtat atctattgac tgattgagta
    10501 tgtcgttcat gtcgttgagg aattttgctg gatgtgctgt ctccaataaa acaccgattg
    10561 tgtctggatg tttttgctgg tactcgccta aagcaaggta ggctacggca ccgtgcgggt
    10621 caatgatgta gttgtgtttt gagtaaactt cttgaacggc ctttcttgtt tggctgtcat
    10681 tgtatgcgta gccagagata gaggcggtca tattgttcca cgtggaacaa ttctctttgg
    10741 aaaagaagtc cagcatacga gcgaaa
//
LOCUS       DAORLF010000115        10759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3291_length_10759_cov_2.335202, whole genome shotgun sequence.
ACCESSION   DAORLF010000115 DAORLF010000000
VERSION     DAORLF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_10759_cov_2.335202"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..>831
                     /locus_tag="PK198_10860"
     CDS             123..>831
                     /locus_tag="PK198_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF39282.1"
                     /translation="MKRGIPAIRLMSLICNRLIFLLLIIFFGSFSTATAQSFKVSGMV
                     VSQEDKQALIGATIVEKGTNNGTITDVDGSFALTLASGQATLLVSYVGFETQTIAVQN
                     RPFIEVALGPEYKLMQEVVVTGYKREIRSDVTSAIASVKSRDIGKLVVTGIDQALQGQ
                     APGVVVTQVTGSPGDDISVRIRGVGTLGNNNPLYVIDGIPTTSGLNMFSITDIESVEV
                     LKDAAAAAIYGARAANGV"
     assembly_gap    832..970
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <971..>2491
                     /locus_tag="PK198_10865"
     CDS             <971..>2491
                     /locus_tag="PK198_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39283.1"
                     /translation="IANANLLRNPANQLPSYDPSILDTLPDNNWLDMLFNPAPVQRYA
                     LSAKGGSEAGNYYISGEYFSQDGIFKGQGFDKYQVRFNGEIGKRLFRVGNQLAFSHTD
                     RKVINGSGDGFGPGNELSGIRYALIASPVFPARYPDGSYINVTSDLGDPVLFGDGNAN
                     PLAFIENTDWTIKRYRVFGSVFAELNPLDGLRLRSTIGGDFLFEREKLFKKRLSPAVY
                     DPTSLNEGRVFNQTLVWNNTIDFNRTFGGHRIAVLAGMEAIQNHTDYLGASANNFRRT
                     DPLFRYISASIPAELNNIGASGIATEWALLSWFGQVGYSYKSRYGLSAAVRRDGSSRF
                     GKNNRWGTFPSFSAAWNISNEPFFQNVRFISSLKIRGSWGQLGNQEIGIYPYSSLVNI
                     GEFVYTFGGSAATGATVVETGNSNIRWETSTQQNIGLDLSLKQDRLSLTLDAFRKRTD
                     DILVRVPVPQAGGATRPPFVNAASVENKGLEAALIYRNKIGGWNYNIGANITTVRN"
     assembly_gap    2492..2511
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2512..3273
                     /locus_tag="PK198_10870"
     CDS             <2512..3273
                     /locus_tag="PK198_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39284.1"
                     /translation="EPILGGFGLSDGPITRTEPGHPIGSFFLWQMTGLFQSQEEIAAA
                     PFQTQFTRPGDVRFADINGDNKIDDKDRLHVGNPFPDFTYGVSLGISNKIFDFSALVQ
                     GVQGNDVYFLYGNFAYETQLRGFNSYQDLLRRWTPDNPNTDIPKVSVDDRNGNRRVST
                     RFLLDGSYMRVRNVTMGCNLKDVLQWKGIGSLRLYVTVQNALTFTKYPGLDPEIQANA
                     NDTRGLGLSSDLSVGIDWGTVPAPRSWIAGIQVEF"
     gene            3288..4760
                     /locus_tag="PK198_10875"
     CDS             3288..4760
                     /locus_tag="PK198_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF39285.1"
                     /translation="MNQMKNFVNLLFTPASTLLTGLLASLLLLNACNGILDKEPIATL
                     DAGSFFQSEADAVQALNAAYRPLLFNNANGNFYWAFAVITADEAITGGDGSRPGLVEL
                     DAFTYTPRTEEFNTFWRLQYQGITQCNLVLDNIDKIDMAQARRGQIRGEALFLRSYYY
                     FLLAQVFGDVPLFTKVTPPDQLKVPRTPKAQVYAQIQRDCEEAAALLPAVQPPASTGR
                     ATRGAAYALAAKSYLYESKWDKVLENVAKVKDLGVYDLMADYRHNFEINTQNNAESVW
                     EIQHTGIGLGLGNFLNQWWMSRKLLGYGFAEVTPAFANGFETGDPRRRFTVAANNDPY
                     FGATYKNSFSSTFFSPRKYLQDTASVAQAPDGDINYTAIRFAEVLLWEAEALNELGRT
                     ADAQAPLERVRARARAQATTPATALPAVTTANQEEMRQAIRRERKAELGFELHRFFDL
                     VRWGIAKDVLPGFQAGKHEVFPIPQTEMDLNAQLVQNAGY"
     gene            4787..7906
                     /locus_tag="PK198_10880"
     CDS             4787..7906
                     /locus_tag="PK198_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF39286.1"
                     /translation="MKRFYNHIVICFLGCIQVAFGQAGQLAVPRVDMMPNQPTPYNVR
                     DWKQVAMRYDSVVYDVQKTGQYLPLTFINPAGVNYPQRPSFRMHTYVGTNSPFGNEAI
                     NVLPSLVGASLVGIDKSNQFGRNWVLMAQDFFNKNNGENLYLNAPSTTSGQDWWYDMM
                     PNVYFYQLYDLYPDMGGEAGFQFTNIADRFAQAVRAMGGNDAPWQPAYMNYRAWRFAT
                     MQPNATGVPEPEAAGAFAWVLYHAWKETGNAEYLKAAEWSLEFLNEWAFNPSYELQLP
                     YGTLIAAKMNAELGTGYNVGKMLNWSFDRGNLRGWGTITGTWGGFNVSGLVGEANNAG
                     NDYAFQLNGVQQAAALAPMVRYDKRYARAVGKWMLNLSNATRLFYPGFLPANLQDASA
                     WSAIHDPDQVIGYEALRQVWQGNSPYSTGDAVNGGWAATNLALYGSSSIGYLGAIVEK
                     TNVDRILKIDVLKTDFFREEAYPTFLFYNSYSTARSVQWAVGDAASDIYDALSGNFIL
                     QNVSGVVNLNIPANEAVLVSICPAGGVITYDKNKMLVNGVVVDYRQNAQPYTAAPRIK
                     SLASTANPLQIGAGATLYCTVEDADSEQFSYQWTTNAGAITGTEGEVSFTAPTQAGEV
                     RIEVIVADPQGNADTAVLLLEIVPEINLPPVIAGIQKSAPYAAPGETLQLSCQAADPN
                     NDAITYAWTASGGSFSGSGSSLNWTAPAAQGIYQISVTASDPAGLSASASTQMLVKIF
                     NPTPGNLIAHYPFTGNAQDVSGNQLHGQAMGAVLTQDMFGTAQRAYYFNGGSQHISVA
                     NSPLLNFQNGITVSCWFNPGALPDRESFLLSHGSWQNRWKLSITPSRLLRWTINSVSG
                     IADLDALIPLKTDTFYHLAATYDGSTMALYLGGELHSFKALSGLMRTAALPFLMGQML
                     PGDANYNFRGVIDEVKIFNYALVPESVNMLYQQEVTSATDGRAQQAGRLMVFPNPAAD
                     HIQAVLPDHGGLVQHVLCYDIAGRIVLEQRDLGGSQMVLDISALPPGAYTIHALSERR
                     RYTARFVKM"
     gene            7942..>8498
                     /locus_tag="PK198_10885"
     CDS             7942..>8498
                     /locus_tag="PK198_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRF39287.1"
                     /translation="MRQYLLLMFVWLLGLAGTAYGQTPAAHYAFTGHAKDGSGFANHA
                     AVNGANLTQDRFGIANHAFFFDGEQSGLTAPNAAHLNSANATISFWINPASFPAQGEA
                     YLLSFGGWQQRWKISLPGHGKPVFTTHSNGACCSDMDSNSPLPANVWTHVTMVHNGTQ
                     NIIYFNGVEVNQKSVNGALDPTTHP"
     assembly_gap    8499..8508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8509..>10759
                     /locus_tag="PK198_10890"
     CDS             <8509..>10759
                     /locus_tag="PK198_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39288.1"
                     /translation="IGYDPIDNANYFHGALDEVMLFDQALSAAEIAALYNQQNTPPTT
                     DDSTVASYAFKGNGADGTPFGNHLDLSTAALSTDRFGFGKSALLCNGQSSEAKAPNSN
                     QLNSPYATVSFWVKVNNLPGNGEAFLLSFGGWQERWKISLPPHGKPVWTTNHSNGISD
                     MDSGDGNALQPGVWTHVTMVHDGAKDKIFINGAQAAEKNVAGTLNSTTHILGLGYNPI
                     DGGNWFDGSLDDIQLYNYALSDAEIAALYAAQAAFPGTPSNLVAHYSLNGDGNDDTPL
                     SNHAALENGAAAVANRHDWASNALEGAALADNSPALQSDHTTISFWVRPNSYPGSGEV
                     YLMSNGGWQERWKISMPSHGKPVFTTHSGGACCADMDSGGNNSLPIGEWTHVVMVHDG
                     EKDKIFFNGVLMNERNSPGALDKTKHPLGIGFDPIDGGSYFDGAFDDVQIYNTALSNA
                     EIAALYDAQNQAPTVGGNLVAHYAFSGNSRDETAYSNHASASVPGKDRFGKANKAAAF
                     DGEFTEVKADNSPQLNSPQTTVAFWVRPNSLPGNGEAFLLSFGGWQERWKISLPPHGK
                     PVWTTNHTNGISDMDSGDGNALEVGEWTHVAMVHDGLKNLIYMDGALVAQKDVVGNLN
                     NTTRSFGMGYNPIDGGNWFDGLLDEVQVYNTALSAAEIAALYTAQSQDGGSGDDEAPC
                     SPLNLTAGVSFNNVSLDWLPATDNVGVVAYNVYIDGVTAISTAQTTAYFPGLTPLRQF
                     EFGVTAIDEA"
BASE COUNT         2407 a         3155 c         2804 g         2224 t
ORIGIN      
        1 ttgagcggcg tgatttcttt ataaatgttc attcacaaaa gtactattta tttgcaatat
       61 tataccataa tcgcttctct cggcggctta tctttgccta taccaataaa accggcgaca
      121 ccatgaaaag aggcatacct gcaatccgtt tgatgagtct gatctgcaat cggttgattt
      181 tcttgttgtt aatcatcttt tttggcagct tttcgacagc tacggcgcag tcgttcaaag
      241 tatccggcat ggtcgtttct caggaagaca agcaagctct gatcggcgcc accatagtag
      301 aaaaaggcac caacaacgga accatcaccg atgtggacgg gagctttgcc ctcaccctcg
      361 cttcggggca ggcaacgctg ttggtcagtt atgtggggtt tgaaacccag accattgccg
      421 tacaaaaccg cccattcata gaagtagcgc tggggccgga atacaagctc atgcaggagg
      481 tggtggtgac gggctacaaa cgcgagatac gcagcgacgt aacctcggcc attgcatcgg
      541 tcaaatccag agacatcggc aaattggtcg tcaccggcat agatcaggca ttgcaggggc
      601 aagcccccgg cgtagttgtc acacaggtga ccggctcgcc cggcgacgac atatcggtgc
      661 gcatacgcgg cgtgggtaca ttgggcaaca acaacccgct gtatgtgata gacggtatac
      721 ctactaccag cggcctcaac atgttttcca tcacagacat cgaatcggtg gaagtgctga
      781 aagacgcggc tgctgcagcc atttacggcg cgcgggctgc caacggcgtg gnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn ccattgccaa tgccaacctg ctgcgaaacc ctgccaacca actgccctct
     1021 tacgacccct ccatactcga tacgctgccc gacaacaact ggctcgacat gcttttcaac
     1081 cctgcccccg tgcagcgtta tgcgctttcg gccaaaggcg gcagcgaggc cggcaattac
     1141 tacatctccg gcgagtattt ttctcaggac ggcattttca aaggtcaggg cttcgacaaa
     1201 taccaggtgc gtttcaatgg cgaaatcggc aaacgcttgt tccgcgtggg caatcagttg
     1261 gcattttctc ataccgaccg caaggtcatc aacggctccg gcgacggatt cggccccggc
     1321 aacgaactga gcggcatccg gtacgcgctc atcgcatcgc ctgtgtttcc ggcgcgttac
     1381 ccagacggca gctacatcaa cgtcacttct gatctgggcg accccgtgct ttttggagac
     1441 ggcaacgcca atccgctggc attcatcgaa aacaccgact ggaccatcaa gcgctatcgc
     1501 gttttcggca gcgtgtttgc cgagttgaac ccgctggacg ggctgagact gcgcagtacc
     1561 atcggcggcg atttcctgtt tgagcgcgaa aaactgttca aaaaacggtt gtcgccggca
     1621 gtttacgacc ccacttcgct caacgagggc agggtgttca atcagacctt ggtgtggaac
     1681 aataccattg acttcaaccg cacgttcggg gggcatcgca ttgccgtgct ggccggtatg
     1741 gaggccatcc aaaatcatac ggattacctc ggcgcatcgg ccaacaactt ccggcgcacc
     1801 gacccgctgt tccgatacat cagcgccagc attccggcgg aactcaacaa catcggcgca
     1861 tcgggcattg ccaccgagtg ggcactcttg tcgtggttcg gtcaggtggg atattcctac
     1921 aaaagccgct atggactgag cgccgccgtt cgccgcgacg gctcttcccg cttcggcaaa
     1981 aacaaccgct ggggcacctt cccttctttc tcggctgcct ggaacatatc caacgagccg
     2041 ttttttcaaa atgtccggtt catttccagc ctgaaaatac gcggaagttg ggggcagttg
     2101 ggcaatcagg aaataggcat ttatccttac agttccctcg tcaatatcgg agagtttgtt
     2161 tacaccttcg gcggcagtgc ggccaccggc gccaccgtgg tggaaaccgg caacagcaac
     2221 atcaggtggg aaacgagcac acagcaaaac attggtttag acctcagtct gaaacaagac
     2281 cggctcagcc tcacccttga tgctttccgc aaacgcaccg atgatattct cgtgcgcgtg
     2341 cccgttccgc aggccggcgg cgctacccgg ccacctttcg tcaatgcagc ctcggtagaa
     2401 aacaaaggtt tggaggccgc tctcatttat cgcaataaaa taggcggatg gaactacaac
     2461 atcggcgcca acattaccac cgtgcgcaat cnnnnnnnnn nnnnnnnnnn ngagccgata
     2521 ctgggcggct tcggcctgtc cgacggcccc atcacccgca cagagccggg ccatcccatc
     2581 ggttcgtttt tcctgtggca aatgaccggg ctgtttcagt cgcaggagga aattgccgcc
     2641 gctccttttc agacgcaatt cacccggccg ggcgatgtgc gctttgccga catcaacggc
     2701 gacaacaaga ttgacgacaa agaccgcctc catgtgggca atccttttcc tgattttacc
     2761 tacggcgttt cgctgggcat cagcaacaag atattcgatt tttcagccct cgttcaaggg
     2821 gtgcagggca atgatgttta tttcctttat ggcaattttg cttatgaaac tcaactgcgc
     2881 ggcttcaatt cctaccagga cctgctccgg cgttggacgc ccgacaaccc caataccgac
     2941 atacccaaag tgagtgtgga cgaccgcaac ggcaaccgcc gggtttctac ccgtttcctg
     3001 ctcgacggct cgtacatgcg cgtcagaaac gtgacgatgg gctgtaatct caaggatgtg
     3061 ctccaatgga aaggcatcgg cagcctgcgc ctgtatgtga cggtgcaaaa tgccctcact
     3121 ttcaccaagt atcccggcct cgatcctgaa attcaggcca atgccaacga cacgcgcggt
     3181 ttggggcttt cttccgacct cagcgtgggc attgactggg gtacggtgcc cgcgccccga
     3241 tcatggattg ccggcattca ggtggaattt tgattaaaat cctgaaaatg aatcagatga
     3301 aaaacttcgt gaaccttctt tttactcccg caagcaccct tctgaccggg ctgctcgcat
     3361 cgttgctgct gctcaatgcc tgcaacggga ttttggacaa agagccgatt gccactttgg
     3421 acgcaggttc cttttttcaa tcggaggccg atgccgtgca ggcgctcaat gccgcctatc
     3481 gcccgctgct gttcaacaac gccaacggca atttttactg ggctttcgcc gtcatcactg
     3541 ccgatgaagc catcaccggc ggcgacggtt cgcgccccgg attggtagaa ctcgacgctt
     3601 ttacctacac tccgcgaacg gaggagttca acactttctg gaggttgcaa tatcagggca
     3661 tcacgcagtg taatctagta ttagacaaca ttgacaaaat agacatggcg caggctcggc
     3721 gcggccagat tcggggcgag gcgctgtttt tgcgctccta ctactatttt ctgttggcgc
     3781 aggtattcgg agatgtgcct ttgtttacca aagtgacgcc gcccgaccaa ctcaaagtgc
     3841 cgcgcacgcc taaagcgcag gtgtatgccc aaatacagcg cgactgtgag gaggccgccg
     3901 ctttgcttcc tgccgtgcag ccgcccgctt cgacaggcag agccacccga ggcgccgcgt
     3961 atgcgctggc cgccaagtcg tatttgtacg aaagcaaatg ggacaaagtg ctcgaaaatg
     4021 tggccaaagt gaaagacctg ggcgtgtatg atctgatggc cgactatcgc cacaattttg
     4081 aaatcaacac gcaaaacaac gcggagtcgg tgtgggaaat acagcacacc gggatagggc
     4141 tgggcttggg caatttcctc aaccagtggt ggatgtcgcg caaactcctc ggctacggct
     4201 ttgctgaagt gacgccggct ttcgccaacg gctttgaaac cggcgacccc cgtcgcagat
     4261 tcaccgtagc cgccaacaat gatccttatt ttggggccac ttacaaaaac tcgttttcct
     4321 ccactttctt cagcccgcgc aaatatcttc aggacaccgc ctccgtagcg caggcccccg
     4381 atggagacat caattacacg gccatccgtt ttgcagaagt gctgctatgg gaagccgaag
     4441 cgctgaacga attggggcga actgccgacg cgcaggcccc gctggaacgc gtgcgcgccc
     4501 gtgcccgcgc tcaggcaacc acccctgcta ctgccttgcc cgctgtgaca acggccaatc
     4561 aggaagaaat gcggcaggcc attcgccgcg agcggaaggc cgagctgggc ttcgagttgc
     4621 accgcttttt cgatctggtg cgatggggca ttgccaaaga tgtgttgccc ggtttccagg
     4681 caggcaaaca cgaggtattc cccatcccgc aaacagagat ggatttgaat gctcagttgg
     4741 tgcaaaatgc gggatactaa attgatttac agcgatatat cgtcttatga aaagatttta
     4801 taaccatatc gtaatatgct ttcttggctg catacaggtc gcattcgggc aggcaggcca
     4861 gctcgccgtt ccccgcgtgg acatgatgcc caaccaaccc acgccctaca atgtgcgcga
     4921 ctggaaacag gtggccatgc gctacgattc cgttgtgtat gatgtgcaaa aaaccgggca
     4981 atacctgcct ttgaccttca tcaatcccgc cggcgtcaac tatccgcaac gaccgtcttt
     5041 cagaatgcac acttatgtgg gtaccaactc gcctttcgga aacgaggcca tcaatgtact
     5101 gccctctttg gtgggtgcaa gtttggtcgg gattgacaaa agcaatcaat tcggcagaaa
     5161 ctgggtgctg atggcgcaag atttttttaa taaaaacaac ggcgagaacc tctatctcaa
     5221 cgcgccctcc accaccagcg gccaagactg gtggtacgat atgatgccca atgtgtattt
     5281 ttatcaactg tacgacctct atcccgatat ggggggcgag gccggatttc agtttaccaa
     5341 tattgccgac cgttttgcgc aggccgtgcg ggctatgggc ggaaacgacg cgccctggca
     5401 acccgcctac atgaactacc gcgcctggag gttcgccacc atgcagccca atgccaccgg
     5461 cgtaccggaa cctgaagctg ccggggcttt tgcctgggtg ctgtaccatg cctggaaaga
     5521 aacgggcaat gcagagtacc tcaaagcagc ggagtggtcg ctggagtttt tgaacgaatg
     5581 ggctttcaat ccatcctacg aattgcaatt gccctacggt acgctcattg ccgcgaaaat
     5641 gaacgccgaa ttgggtaccg gctacaacgt ggggaaaatg ctcaactggt cattcgaccg
     5701 gggcaacctg cgcggctggg gcaccatcac ggggacatgg ggcggcttca atgtatcggg
     5761 attggtgggc gaagccaata atgcaggcaa cgattacgcc ttccaactca acggcgtgca
     5821 gcaggcggcg gcactcgctc cgatggtgcg ctacgacaag cgctatgccc gagccgttgg
     5881 caaatggatg ctcaatctca gcaatgccac ccgcttgttt taccccggtt tcctgcccgc
     5941 caacttgcag gatgcctcgg catggtcggc cattcatgac cccgatcagg tgataggtta
     6001 cgaagcgttg cggcaggtgt ggcagggcaa ttccccgtac tctaccggcg atgcggtgaa
     6061 cggaggctgg gccgccacca atctggcttt gtacggttct tcttccatcg gttatctggg
     6121 ggcgattgtt gaaaaaacca acgtggacag aatcctgaaa atagacgtac tgaaaaccga
     6181 ttttttccgc gaggaggcct atcctacctt tttattttac aactcttaca gcaccgcccg
     6241 gtctgtgcaa tgggcagtgg gcgatgccgc atcggatatc tacgatgccc tttccgggaa
     6301 ttttattttg caaaatgtga gcggcgtcgt caacctcaac attccggcca acgaagccgt
     6361 attagtcagt atatgcccgg ccggcggcgt cattacttac gataaaaaca aaatgctggt
     6421 caacggcgtg gtggtggact atcgccaaaa cgcccagcca tacaccgctg ctccgcgcat
     6481 caaatccctc gcctcaacgg ccaatccgct gcaaatcgga gccggcgcca ccctgtactg
     6541 cacggtggag gatgccgact ctgaacaatt cagttaccaa tggacgacca atgccggcgc
     6601 cataacgggc accgagggcg aggtgagttt caccgcgccg actcaggcgg gcgaggtgcg
     6661 catcgaagtc attgtggccg atccgcaagg caatgcagat accgctgtgc tcttgttgga
     6721 aatcgtaccc gaaatcaatc tgccgccggt cattgccggt attcaaaaaa gcgcgcctta
     6781 tgccgctccg ggcgaaaccc tgcaactgag ctgtcaggcc gccgacccca acaacgacgc
     6841 cattacttac gcctggacgg cctccggcgg gagcttcagc ggatcgggca gcagcctgaa
     6901 ctggaccgcg cccgccgctc agggcattta tcaaatatcg gtgacggctt ccgatcctgc
     6961 cggtctgagc gcatcggcaa gcacgcagat gctcgtaaaa attttcaatc cgacgccggg
     7021 caacctcatc gctcactatc ccttcaccgg caatgcccag gatgtgagcg gcaatcagtt
     7081 gcacgggcag gcgatgggcg cggtactcac ccaagacatg ttcggcacgg cccaaagagc
     7141 ctactatttc aacggcggtt cgcagcatat ttcggtggcc aacagtcctt tgctcaattt
     7201 tcaaaacggc atcacggtga gttgctggtt caatcccggc gcattgcccg acagggaatc
     7261 cttcctgctc tcgcacggca gttggcaaaa ccgctggaag ctctccatca cccccagccg
     7321 gctgctccgc tggaccatca actcggtgag cggcattgcc gacttggacg ccctcatacc
     7381 gctcaaaacc gacacctttt accatctggc cgccacctac gacggcagca cgatggcgct
     7441 gtacctcggc ggagaactgc acagtttcaa agccctcagc ggattgatgc gcactgccgc
     7501 cctgcccttc ctgatggggc aaatgctgcc gggcgatgcc aattacaact tccggggcgt
     7561 gatagacgag gtgaaaattt tcaactacgc gctcgtcccg gaatccgtca acatgctgta
     7621 ccaacaggaa gtgacctccg cgacggacgg cagggcgcaa caagcgggcc gactgatggt
     7681 atttcccaat ccggctgccg accacattca ggctgtattg cccgatcatg ggggccttgt
     7741 tcagcatgtt ttgtgctatg acatcgccgg tcgtattgtg ttggaacaaa gggatttagg
     7801 cggaagccag atggtcttgg atatcagtgc tctgccgccg ggagcataca ccattcacgc
     7861 cctgagcgag cgccgccgct acaccgcccg ctttgtcaaa atgtaaccat ttattcacta
     7921 acaataaaat cggaaataac aatgaggcag tatctattac tcatgtttgt atggcttctt
     7981 ggcttagccg ggactgccta cgggcaaacg cctgccgcgc attacgcctt tacgggccat
     8041 gccaaagacg gttcgggttt tgccaaccat gctgctgtca acggagcgaa tctcacgcag
     8101 gaccgcttcg ggatagccaa ccacgccttc tttttcgacg gcgaacagag cgggctgacg
     8161 gcgcccaatg ccgcacatct caattcggcc aacgctacga tcagcttctg gatcaacccg
     8221 gcatcgtttc cggcgcaggg cgaagcctac ctgctctcct tcggcggctg gcaacagcgc
     8281 tggaaaattt cattgcccgg ccacggcaaa cccgttttta ccacccactc caacggtgct
     8341 tgttgcagcg acatggactc caactcgccg ctgcctgcca acgtgtggac gcatgtgacg
     8401 atggttcaca acggcacgca gaacatcatc tatttcaatg gtgtagaggt gaatcagaaa
     8461 agcgtgaacg gcgccctcga tcccaccacg caccccttnn nnnnnnnnat cggttatgat
     8521 ccgatagaca atgccaatta ttttcatggc gcgttggacg aggtgatgct cttcgatcag
     8581 gctttgagcg ctgccgaaat tgccgccttg tacaaccagc aaaacacgcc tcctacgacg
     8641 gacgacagca cagtggcctc gtacgccttc aaaggaaacg gcgccgacgg cacgcctttc
     8701 ggcaaccacc tcgacctgag taccgctgcc ctgagcaccg accggttcgg gttcggaaaa
     8761 agcgcactgc tgtgcaacgg ccaatcctcg gaggccaaag ctcccaacag caaccaactc
     8821 aactcgccgt acgcaacggt cagtttttgg gtgaaagtga acaacctgcc cggcaacgga
     8881 gaagccttcc tgctctcctt cggcggctgg caggaacgct ggaaaatctc gctgccgccc
     8941 cacggtaaac ccgtgtggac caccaatcac tccaacggca tctccgatat ggacagcggc
     9001 gacggcaacg ccctgcaacc cggcgtatgg acgcatgtaa cgatggtaca cgatggagcc
     9061 aaagacaaga tttttatcaa cggcgctcag gctgctgaaa aaaatgtggc aggcacgctc
     9121 aacagcacca cccatattct gggcctcggc tacaatccga tagacggcgg caactggttt
     9181 gacggctcgc ttgacgacat acaactctac aattacgccc tctccgacgc agaaatcgct
     9241 gccctgtatg ccgctcaggc tgctttcccc ggcacgcctt ccaacctcgt agcgcactac
     9301 agcctcaacg gagacggcaa cgacgatact ccattgagca accatgctgc cttggaaaac
     9361 ggcgctgctg ctgtggccaa tcgccacgac tgggcctcca atgccctgga aggcgcggct
     9421 ctggccgaca actcacccgc tttgcagtcc gatcatacca ccatcagttt ttgggtcagg
     9481 cccaacagct accctggcag cggcgaggtg tatctgatgt cgaacggcgg ctggcaggag
     9541 cgctggaaaa tctccatgcc ttcgcacggc aagcccgtat tcactaccca ctccggcggc
     9601 gcctgctgtg ccgatatgga ttcggggggc aacaactccc tgcccatcgg cgaatggacg
     9661 catgtcgtca tggtgcatga cggcgaaaaa gacaaaattt tcttcaacgg cgtgctgatg
     9721 aacgagcgca attcgcccgg cgctttggac aaaaccaaac atccgctggg catcggcttc
     9781 gatcccattg acgggggcag ctacttcgac ggcgcgttcg atgatgtgca gatttacaac
     9841 acagccctga gcaatgccga aattgcggcc ctgtacgacg ctcaaaatca agcacctacg
     9901 gtgggcggta atttggtggc gcattacgcc ttcagcggca acagccgcga tgaaaccgcg
     9961 tacagcaacc acgcctccgc ctccgtgccc ggcaaagacc gtttcggcaa ggccaacaaa
    10021 gccgccgctt ttgacggtga atttacggaa gtgaaggccg acaattcgcc acaactcaac
    10081 tcgccgcaaa ccaccgtagc tttttgggtc aggcccaaca gtctgcccgg caacggagaa
    10141 gccttcctgc tctctttcgg cggctggcag gaacgctgga aaatttcgct gccgccccac
    10201 ggcaaacccg tatggaccac caatcacacc aacggcatct ccgacatgga cagcggcgac
    10261 ggcaacgctt tggaagtggg cgaatggacg catgtagcaa tggtacatga cggcctcaaa
    10321 aatctcatct acatggatgg cgctttggtg gctcagaaag atgtagtcgg caacctgaac
    10381 aacaccaccc gttccttcgg catgggctac aaccccattg acggcggcaa ctggtttgac
    10441 ggcttattgg acgaagtgca ggtttacaac acggccctct cggcagcaga aatagccgca
    10501 ctttacaccg ctcaatcaca ggatggcggt tccggcgacg atgaggcgcc ctgctctccg
    10561 ctcaacctca cggcaggcgt cagtttcaat aatgtcagtt tagactggct gcccgctacc
    10621 gacaacgtgg gcgtagtggc ctacaatgtt tatatagacg gcgttacggc gatctctacc
    10681 gcacaaacca ccgcgtactt cccggggctg actcctctca ggcagtttga gttcggcgtg
    10741 accgccatag atgaggccg
//
LOCUS       DAORLF010000116        10677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3328_length_10677_cov_2.044436, whole genome shotgun sequence.
ACCESSION   DAORLF010000116 DAORLF010000000
VERSION     DAORLF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_10677_cov_2.044436"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK198_10895"
     CDS             complement(<1..514)
                     /locus_tag="PK198_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39289.1"
                     /translation="MRRQRCLLILDGTEPLQYPPGTPGGLGGKLKDLALAALLKELAY
                     GQPGLCLLSTRVPIENLAGIEAPKHLCRRLENFEAHDGALLLKNLGVRGPQRELEAAS
                     AEYKGHALALRLLGNYLVKVLGADVRRRNEIPHLTDEKKDGYHARRVMQAYVHWFEAE
                     NAKPQKLGFWK"
     gene            complement(409..>883)
                     /locus_tag="PK198_10900"
     CDS             complement(409..>883)
                     /locus_tag="PK198_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39290.1"
                     /translation="RKESVLTLFDFKEKLSEIGHFPSVYENEKDLVFQFLEQLEKSDN
                     AGFFPKNEAETRTDISHLPDLQPHFTAREQELALLDAAWADPQTNLLQFVAPGGTGKT
                     MLLTYWLHHYLPESKAERPAACAGSAACSSSTARSRCNTRPAPPADWAANSKTSH"
     assembly_gap    884..997
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<998..1247)
                     /locus_tag="PK198_10905"
     CDS             complement(<998..1247)
                     /locus_tag="PK198_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39291.1"
                     /translation="MQPIKIFLASSSELEADRKAFAECIRRKNQAWHERGIFLELLIW
                     EDFLDAVSKTRSQDEYNKAVRASDIFVMLYFSKVGKYTF"
     gene            1687..3483
                     /locus_tag="PK198_10910"
     CDS             1687..3483
                     /locus_tag="PK198_10910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF39292.1"
                     /translation="MKSLLTFVSLLLLSSPICCQNKYSAETLEKIKEVENNITGGIVV
                     NDARPNTIMEQMAKYKVNGMSIAVIHDYKIAWAKGYGWADVAEKRPMTTKTLFEPGSI
                     SKTLNAVGILKLAQDKKVDLYADINTYLSSWKFPYDSLSKGKKITLAQLLSHQAGLTV
                     HGFPGHDINGPIPTVYEVLDGKKPSFTPAVRSAFEPDLRFQYSGGGTTISQVLLSDVI
                     RQAYDVWMYKNVLKPIGMVNSTYAQPPAKSKQRWCSSAYNGDGSPIERKFHVYPEQAA
                     AGLWMTPTDLCRYIIDMQLAWKGQASRVLNPDMVKLHLTPYNNGPAGMGTFIDNIDGA
                     IYFQHGAGNDGFCGQFFGSLEDGYGVAIFLNSDNGRLLGEVINSVAKAYNWKNFYRAP
                     RQVKSIAVSNELLKTYEGIYLFDDTWAAIGKKDNEFHFFTSGTHAKMYFTTPTQFVNE
                     EFQAVKTMIKDEKGNVTGYTRSVNGREYPAAQKVVGIDTAQLANPILMGVGWYYFGNK
                     QYKESLTTFQRGMQLYPDDLNMYMNAAHLYLFNGDYGAALAIHKKHLNDMIRPGYSWV
                     DSLKDDYNYLKSTGEDLKMFDRLLEELRIDKP"
     gene            3691..4317
                     /locus_tag="PK198_10915"
     CDS             3691..4317
                     /locus_tag="PK198_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF39293.1"
                     /translation="MNLTDQHNEAKTSILRKAEMLFMRLGLKSISMDDIARELGISKK
                     TLYQFFQSKEDLVLQTISRHQCTEAEEVKRLCETSTDALKEMLDIARHVLVQFQQMSP
                     STVFDLKKYYPEAWRIVEQVQREYVYGVLKSNMERGIREGLYRDDFDANILARLYVGS
                     VRWLLDEELFPQTQFHWAQLYREFIHYHLRGIVSQKGLEKLKAYIQDL"
     gene            4380..5705
                     /locus_tag="PK198_10920"
     CDS             4380..5705
                     /locus_tag="PK198_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF39294.1"
                     /translation="MLWLVFVPMLCLSPLAAQQSLTMQNSVQFALQNNPQVQKAALEV
                     QKGDYQIKEYLAVRLPQLNGSGNFTFNAKLPTQLIPNFFGGNPDELLPVQFGTALSVS
                     AGLELSQLVYDHGVWLGVKATDKLATFNRLIEAKTQEDVAYNVVKIYMQAQILGKQRG
                     ILEANLLQVEGLLRATELQFQNGFAKKLDVDQLRVNRSTLRTQLDNLNLQYEQSLRAL
                     KFAMAMPLDAPITLADTLQDSSLPTLQTDATPGFGNKIDLAIVNQQRQLLDLQLAQTR
                     ANYLPSARFFANYAVQGQGNGFGDLGDGNRWFDYAIMGINLRVPIFDGFRKGHRLQQN
                     QIELLQNEKDRQLTQNSLEFQYQNAQLQLQSSFNSLRSQKENLAMAEEVYAVMQKRFR
                     EGIAPITEVLNAETSMREVQTNYLAALLQYKWASLDLEYANGKLLQLFK"
     gene            5783..>6124
                     /locus_tag="PK198_10925"
     CDS             5783..>6124
                     /locus_tag="PK198_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRF39295.1"
                     /translation="MKKVIRILITLLVAGAALAAIAWKLGENKKVINEQAQLAQERND
                     VIPVKTAALGRQDVRADFEASGVFQPFKELALISDVQGRITQLNVANGSFVNEGAVIL
                     AVDNDLLKNQLD"
     assembly_gap    6125..6144
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6145..6871
                     /locus_tag="PK198_10930"
     CDS             <6145..6871
                     /locus_tag="PK198_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF39296.1"
                     /translation="KVNLKKAESDLSRLKNLVGEGGVTQQQVNDAEIAVENLKAQIKG
                     FDKQLALTVVKAPISGVVSGKTIEKGAYIGPGMQILNIVNVGRLKFQVNLREEEVVQV
                     KTGQKVELSADLYPDRKFVGVVTYISVKSDNSKRFLVEIELDNPAGAPLKAGMNGHAF
                     LSSGQSVNVLALPREAIVGSVRDAQVFVVENNTAKLKKVELGNIYGDFASVRSGLQNG
                     DVVVISGQINLEDGMKVTVEGKQ"
     gene            7075..10242
                     /locus_tag="PK198_10935"
     CDS             7075..10242
                     /locus_tag="PK198_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF39297.1"
                     /translation="MSITEIAIKRPSLIIVAFTVLTILGLISYSKLSYELIPKFSAPI
                     VFISTVYPGASPGEVENNLTKVIEDAVSGIEKISGIRSTSFESLSLITIEFKQDAKID
                     VLLQETQRKINAIEATLPADAKAPVISKFASDEFPIMSIAGNADLPNTALYDLMKNRI
                     KPALSRVEGVGQITLVGGQEREIQVQVNRAKLEQNNLSLLQIVQAIQAANLEFPTGKI
                     KSDKEQILVRLAGKIQTLDDLRNLSLFTLPNGVSIKLSDVAEVQDVAKDADDLNRLNG
                     RGAIGILVQKQSDANAVAVSAGVKAEMEKLQKVYEAEKLSFKIGNDSTDFTNAAVDAV
                     VHDLGLAIILVALVMLVFLHSFRNSLIIMVAIPASLVSTFIGMYLFDFTLNLMTLLAM
                     SLVIGILVDDSIVVLENIYRHMEMGKNRRVAALDGRNEIGFTALSITLVDVVVFVPLA
                     MSGGIVGNIMGQFAWVVVFSTLMSLVVSFTVTPMLASRFSKIENLSSKSIFGAFFIAF
                     EKMMHGLTETYARLLKWSLRHKAIVLGVTTLLFFGSFALVGGGFIGTAFFGSSDRGEF
                     IIQLELPKSSTLRETNLAAQKAEQLLSQKPEITNLFTTVGRTTGLLGGQSTPYLAEIQ
                     VKMVDLEQRAGVRSDVYGSRVRNELERQLPGVKVTTNIVSFFGGADQAPIQVIVSAGR
                     LEDAMKYAKFLLEKAKKVPGTLEAEITVKEGNPEINVAINRERMAELGLDMQMVGATM
                     QTAFSGNTNAKYRDGANEYDINVRLDAFDRRNLADISRLTFINMRGELIRLDQFAAVT
                     QTTGTSQLERKDRTAAVTVQSKVLGRPSGTVGAEIQEIIAQNPPPAGVSIAYDGDLKA
                     QEEGFGSLGLAFLAAILFVYLIMVALYDNWVYPFVVLFSIPVAIVGALLAMALAMQTL
                     DIFSIVGIIMLVGLVGKNAILLVDFANQMKAEGFGTFDSLILAGRIRLRPILMTTIAM
                     VFGMMPIALAKGAGAEWKNGLAWALIGGLTSSMLLTLVVVPVIYYIMDWIKGDLKRQK
                     RNGVRPIEEYHGNDDHGTGVHFEGS"
     gene            10596..>10677
                     /locus_tag="PK198_10940"
     CDS             10596..>10677
                     /locus_tag="PK198_10940"
                     /inference="COORDINATES: protein motif:HMM:NF022438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2805 domain-containing protein"
                     /protein_id="HRF39298.1"
                     /translation="MTEQDIDRIIEMAWEDRTPFEAIELQF"
BASE COUNT         2621 a         2877 c         2739 g         2306 t
ORIGIN      
        1 ttttccaaaa ccccaatttt tgaggtttgg cattttcagc ctcaaaccaa tggacgtagg
       61 cttgcatcac acgacgggcg tgatagccgt cctttttttc gtcggtcagg tgcggtattt
      121 cattgcggcg acgcacatcg gcgcccagca ccttcactaa gtagttgccc agcaggcgca
      181 gcgccagggc atggcctttg tattctgcgg aggcggcttc gagttcgcgt tgcgggccgc
      241 gcacgccgag gtttttcagc agcagggcgc cgtcgtgtgc ttcaaagttt tccaatcggc
      301 ggcacaggtg cttgggggct tctatgcctg cgaggttttc tatgggcacg cgggtgctca
      361 gcaggcagag gccgggctgg ccataggcaa gttctttcaa caatgcagct aatgcgaggt
      421 ctttgagttt gccgcccagt ccgccggggg tgccgggcgg gtattgcagc ggctccgtgc
      481 cgtcgaggat gagcaggcag cgctgccggc gcaagcggcg ggccgttcgg ccttgctttc
      541 gggcaggtag tggtgcagcc agtaggtgag gagcatggtt ttgccggtgc cgcccggcgc
      601 tacaaattgc agcaggttgg tctgcgggtc ggcccaggcg gcgtccaaga gggcgagttc
      661 ttgttcgcgg gcggtgaagt ggggctggag gtcggggagg tgggagatgt cggtgcgggt
      721 ttcggcttca tttttaggaa aaaagcctgc attgtctgat ttttccaact gttctaaaaa
      781 ctgaaaaacg aggtcttttt cattttcata aaccgacggg aaatgcccga tttcgctcag
      841 tttttcttta aaatcaaaca aggtcagtac actttccttt cttnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa aggtatattt gcccactttt
     1021 gagaaataga gcatcacaaa gatgtcggag gcgcggacgg ctttgttgta ctcgtcctgc
     1081 gagcgcgttt ttgaaacggc gtcgaggaag tcttcccaaa tgaggagttc gagaaaaatg
     1141 cctcgttcat gccatgcctg attttttcgg cggatacatt cggcaaacgc tttccggtcg
     1201 gcttcgagtt cagaggagga ggcgagaaaa attttgatgg gctgcatgat gtgtcacggt
     1261 tcacttatgg gcataaaagt aataaagccc tgcctaaatc ttgtgcaatt tgacaaatct
     1321 cgaagatttg tcaaattttc actttcccga cgctgaaaga cgcagatttt tatgatcgtt
     1381 atgtcctcta cgtgcagcgg aatcatacag atcatacaag atcagcgtca aaaaaaacga
     1441 cgcggtttgc cccgtacaag tcaggaagta accggaaatt actgcctgac tcgtctccca
     1501 ccattttcac acggcgcctc ccgccgcgtt ggggtgcatc gttagaagct acctgaaacc
     1561 gcccgacagc atttacactt catttacaat cttttacagt aaaacaactg ccttcgccca
     1621 ggatcgcttt atatttgtga gcttgtcaag ctacttcttt catcatttcc tcacttacac
     1681 catcgtatga agtcattact cactttcgtc tccctcttgc tgctgagcag tcccatttgt
     1741 tgccaaaaca agtactccgc cgaaacgctc gaaaaaatca aagaggtgga gaacaacatc
     1801 accggaggca tcgtcgtcaa cgacgccagg ccaaacacca ttatggaaca aatggccaaa
     1861 tacaaggtga acggcatgag catcgcggtc attcatgatt acaaaatcgc gtgggcaaaa
     1921 ggatacggtt gggcagacgt ggcagagaaa cgaccaatga ctactaagac gctttttgag
     1981 ccggggtcca tcagcaaaac actgaatgcg gtgggcatac tgaaattagc gcaagacaaa
     2041 aaagtggacc tctacgcaga catcaacact tacctcagtt cctggaaatt cccctacgac
     2101 tccctttcaa aaggcaaaaa aataaccctc gcccaattgc tcagccatca ggcgggtttg
     2161 acggtgcatg gctttccggg ccacgacatc aacggaccca ttcccaccgt gtatgaggta
     2221 ttggacggca aaaaaccttc cttcacgccg gccgtgcgca gtgcgtttga gccggatttg
     2281 cggtttcagt actccggcgg cggcaccacc atctcgcagg tgttgctctc cgatgtcatc
     2341 cggcaagctt atgacgtctg gatgtacaaa aacgtgttga aaccgatcgg catggtcaac
     2401 agcacctacg cacagccgcc ggcaaaaagc aagcagcgtt ggtgctcctc agcctacaac
     2461 ggcgacggca gccccatcga acgcaaattt cacgtttacc ccgaacaggc ggcggcgggc
     2521 ctgtggatga cgccaaccga cttgtgccgc tacatcattg atatgcagtt ggcctggaaa
     2581 ggacaagcct ccagggtatt gaacccggac atggtaaaac ttcacctgac gccctacaac
     2641 aatggcccag ccggtatggg cacttttatt gacaacattg acggggctat ttattttcag
     2701 cacggggccg gcaacgacgg gttttgcggg caatttttcg gcagccttga agacggatat
     2761 ggagtggcca ttttcctgaa ttccgataac ggcagattgc tcggagaagt catcaacagt
     2821 gtggcaaaag catacaactg gaaaaacttt taccgcgcac cgagacaggt gaagagcatc
     2881 gccgtgtcga atgaattatt gaaaacgtat gaaggcattt atctttttga tgacacttgg
     2941 gcggccatcg gcaagaaaga caatgaattc cactttttta ccagcggaac acatgccaaa
     3001 atgtatttta ccacgcccac tcagtttgtc aacgaggaat ttcaagccgt aaaaaccatg
     3061 atcaaagatg aaaaaggcaa tgtcacgggc tatacccgct cggtaaacgg ccgggaatat
     3121 cccgctgccc aaaaagtcgt gggcattgat accgctcaat tggcgaaccc gattttaatg
     3181 ggcgtcggct ggtattattt tggcaacaag cagtacaagg aatcgctgac caccttccaa
     3241 cgaggcatgc aactataccc cgacgatctg aatatgtaca tgaatgcagc acatttgtat
     3301 ttgttcaacg gcgattatgg agccgccctt gcgatccaca aaaagcatct caacgatatg
     3361 atcaggcccg gttatagctg ggtggattcc ctgaaggacg actacaacta cctaaaaagc
     3421 accggggaag atttgaaaat gtttgaccgg ttgttggagg agttgaggat tgacaaacca
     3481 taaaaaattg taagaaatat agccggcatc aaagccacaa ataaaggagc aacctactcc
     3541 acttcagacc ttgtaagaat gggccactta caaggtctga accaacctgt tcgtcccccg
     3601 tttcatgccg ttcatctatg cttttaaaag aaactcaaaa aactttggaa acttttaaac
     3661 ctttcagagt ttccttcgtt tttggtcggt atgaatttga cagaccaaca taacgaagcg
     3721 aaaacgtcca ttctcagaaa ggccgaaatg ctgtttatgc gcctcggact gaagagcatc
     3781 agcatggacg acatagcccg cgaattgggc atttccaaaa aaacgctcta ccagtttttc
     3841 caaagcaaag aagacctcgt gctgcaaacc atcagccgcc accaatgcac cgaagcggag
     3901 gaagtgaagc gtttgtgcga gacctccacc gacgctctca aagagatgct cgatattgcc
     3961 cggcatgtat tggtgcagtt tcagcagatg tcgccttcta cggtgttcga tctcaaaaaa
     4021 tactacccgg aggcctggcg catcgtggag caggtgcagc gcgagtacgt ttacggcgtg
     4081 ctgaaatcca acatggagcg cggcatccgc gagggtttgt accgcgacga tttcgatgcc
     4141 aatatcctgg cgcgcctgta tgtaggcagc gtgcgctggc tgttggacga ggagctattt
     4201 ccgcagacgc agttccactg ggcgcagttg taccgcgagt tcatacacta tcacttgcgg
     4261 ggcattgtgt ctcaaaaagg gcttgaaaaa ttgaaagcct acattcagga tttgtaaaat
     4321 cactcattga ctttattatg ataccacccg acaccacctc actacgaaaa cggcccccat
     4381 tgttgtggct cgtttttgtg ccgatgctgt gcctgagtcc gctcgcggcg cagcaatcgc
     4441 tcaccatgca aaacagcgtg caatttgccc tgcaaaacaa tccgcaggta caaaaggcgg
     4501 cgctggaagt ccaaaaaggc gattaccaga tcaaggagta tctggccgtc cgattgcctc
     4561 agttgaacgg cagcggcaat ttcaccttca atgccaaatt gcctacgcag ttgattccca
     4621 acttcttcgg cggcaatccc gacgaactgc tgcccgtgca gtttggtacg gcgctcagcg
     4681 tttcggcggg gctggaactc agccagctcg tgtacgatca cggcgtttgg ctgggcgtaa
     4741 aagccaccga caaattggca acgttcaatc gcctcatcga agcgaaaacg caggaagacg
     4801 tggcctacaa cgtcgtcaaa atttacatgc aggcgcagat tctgggcaaa caacgcggca
     4861 tcctcgaagc caacctgctt caggtggagg gcctgttgcg cgccaccgaa ttgcagttcc
     4921 aaaacgggtt tgccaaaaaa ttggacgttg accagttgcg cgtcaaccgc agcaccctcc
     4981 gcacccaatt ggacaacctg aacctgcaat acgagcagtc tttgcgcgcg ctgaagttcg
     5041 ccatggccat gcctttggat gcgcccatca cgttggccga caccttgcag gacagcagtt
     5101 tgccgacttt gcaaacagac gcaacgccgg gcttcggcaa caaaattgac ctcgccatcg
     5161 tgaaccagca gcggcaactt ttggacctgc aattggccca aacccgcgcg aactacctgc
     5221 catcggcccg cttttttgcc aattacgccg tgcagggcca gggcaacgga ttcggcgatc
     5281 tgggcgacgg caatcgctgg ttcgactacg cgatcatggg catcaacctg cgcgtaccca
     5341 ttttcgacgg cttccgcaaa gggcatcgct tgcagcaaaa ccagatagag ctgctgcaaa
     5401 atgaaaaaga ccgccaactg acgcaaaaca gcctggagtt tcagtaccaa aacgcgcaat
     5461 tgcaattgca aagcagcttc aacagtctgc gctcgcagaa ggaaaacctc gctatggccg
     5521 aagaagtgta tgcggtgatg cagaagcgtt tccgcgaggg cattgccccc attaccgaag
     5581 tgctgaacgc cgaaacctcc atgcgcgagg tgcagaccaa ctacttagcc gcgctgctgc
     5641 aatacaaatg ggccagctta gacctggaat acgccaacgg aaaactcttg caacttttta
     5701 aataatcatc caacccgacg aagccggaac agaggtctta tcactcgttc cggtactccg
     5761 acaacaaccg caaacatcca ccatgaaaaa agttattcgc atcctcatca ccctcctcgt
     5821 ggccggtgcg gcattggctg ccattgcctg gaaattgggt gaaaacaaaa aagtcatcaa
     5881 tgagcaggcc caattggcac aagagcgcaa cgatgtcatt ccggtgaaaa ccgccgctct
     5941 cggtcgccag gacgtgcgcg ccgatttcga ggcatcgggc gtgttccagc cgttcaaaga
     6001 actggcgctc atctcagacg tacagggccg catcacgcag ctcaacgtgg ccaacggttc
     6061 tttcgtcaac gaaggcgccg tcatcctcgc cgttgacaac gacctgctca aaaatcagtt
     6121 ggacnnnnnn nnnnnnnnnn nnnngaaagt gaacctgaaa aaagccgaaa gcgacctcag
     6181 ccgcctcaag aatctggtgg gcgaaggcgg cgtcacgcag cagcaggtca acgacgccga
     6241 aattgccgta gaaaacctca aggcgcagat caagggcttc gacaagcaac tggcgctcac
     6301 cgtggtgaag gccccgattt cgggcgtcgt ttccggcaag acgatagaaa aaggcgctta
     6361 catcggtccg ggcatgcaaa tcctcaacat cgtgaacgta gggcggctca agtttcaggt
     6421 caacctccgc gaagaggaag tggtgcaggt caaaaccggc caaaaagtag aactctctgc
     6481 cgacctctac cccgaccgca agtttgtggg cgtggtcact tacatttctg taaaatccga
     6541 caactccaag cgcttcctcg tggaaattga gttggacaac cctgccggcg cgcccctcaa
     6601 ggcgggtatg aacgggcacg cttttctcag cagcggccag agtgtcaacg tgttggccct
     6661 gccgcgcgag gccattgtgg gcagcgtgcg cgatgcgcag gtctttgtgg tggaaaacaa
     6721 cacggccaag ctcaaaaagg tagaattagg caacatctac ggcgacttcg cctcggtgcg
     6781 cagcggcttg cagaatggag acgtggtggt gatttccggc cagatcaacc tggaagacgg
     6841 gatgaaggtg acggtggagg ggaagcagta ggcgcgccgt ggcgcggagt gggcggtggg
     6901 cagtaggcgg tgggcggttc caacccgaaa cctcgaaccc ggaacccgcc tttgccaagg
     6961 cttcggcgga tgaaacccga aaccttgaac tcggaacatc caatttaaaa aaataaccca
     7021 actcaaagct cgcggctctt ggcaacaagc tggttggtta gaaaaaatga aacaatgtcc
     7081 attacagaaa tagccataaa acgcccgtcg ctgatcatcg tagcgtttac ggtgctcacc
     7141 attttgggac tcatttccta ctcaaaattg agttacgaat tgatccccaa attttcggcg
     7201 cccatcgttt tcatctccac cgtttacccc ggcgcatcgc ccggcgaggt ggaaaacaac
     7261 ctcaccaagg tgatagaaga cgctgtgtcg ggtattgaaa aaatatccgg catccgctct
     7321 acctcgttcg agagcctgtc gctcatcacc atcgagttca agcaggacgc taagattgac
     7381 gtgctcttgc aggaaaccca gcgcaaaatc aacgccatag aggccaccct gccggcagac
     7441 gccaaagcgc ccgtgatctc gaaattcgcc tccgacgagt ttcccatcat gagcatcgcc
     7501 ggcaatgccg acctgccgaa tacagcgctg tacgacctga tgaaaaaccg catcaaacct
     7561 gccctctcgc gggtagaagg cgtggggcag atcaccctcg taggcggcca ggagcgcgaa
     7621 attcaggtac aggtcaaccg cgccaaattg gagcaaaaca acctgtcttt gttgcaaatt
     7681 gtgcaggcca ttcaggcggc caacctcgaa tttcccaccg gaaaaatcaa gagcgacaaa
     7741 gagcagattt tggtgcgcct tgccggcaaa atccaaacct tagacgacct ccggaacctc
     7801 tcgctcttca ccctgcccaa cggcgtttcc atcaagctct ccgacgtggc cgaggtgcaa
     7861 gacgtggcca aagacgccga cgacctcaac cgcctcaacg ggcgcggcgc catcggtatc
     7921 ctcgtgcaaa agcaaagcga cgccaatgcc gtagccgttt ctgccggcgt gaaggccgaa
     7981 atggagaagc tgcaaaaagt gtatgaggcc gaaaaactca gctttaaaat cggcaacgac
     8041 tccaccgact tcaccaatgc cgccgtggac gccgttgtac acgacctcgg tctggccatc
     8101 atactcgtgg ccttagtaat gctcgttttt ctgcacagct tccgcaactc gctcatcatc
     8161 atggtggcca ttccggcctc gttggtgtcc accttcatcg gcatgtacct gttcgatttc
     8221 accctcaacc tcatgacgct gctggccatg tctttggtca tcggtatctt agtggacgac
     8281 agcattgtgg tgctcgaaaa catctaccgc cacatggaaa tgggcaaaaa ccgccgggta
     8341 gccgctttgg acggtcgaaa cgaaatcggc ttcacggccc tgtccatcac tttggtggac
     8401 gtggtggtgt ttgtaccgct ggccatgtcg ggcggcattg tgggcaacat catggggcag
     8461 tttgcctggg tagtggtgtt ttccacgctc atgagtttgg tggtgtcgtt caccgtgacg
     8521 cccatgctgg cctcgcgttt ttctaaaatc gaaaacctga gcagcaagag cattttcggg
     8581 gcctttttca tcgcttttga aaaaatgatg cacggcctca ccgaaaccta cgcccgcctg
     8641 ctcaagtggt ccttgcgcca caaggccatc gtgctgggcg tcaccacctt gctgtttttc
     8701 ggttcgtttg ccttggtggg cggcggtttc atcggtacgg catttttcgg cagcagcgac
     8761 cgcggcgagt tcatcattca gttggaactg cccaaatcct ctaccttgcg ggagaccaac
     8821 ctcgccgcac aaaaggccga gcaactcctt tcacagaaac ccgaaatcac caaccttttc
     8881 accaccgtag gtcgcaccac cggtttgttg ggaggtcaaa gcacgcccta tctggctgaa
     8941 attcaggtaa aaatggtgga ccttgaacaa cgcgccggcg tgcgctccga cgtgtatggc
     9001 agtagggtgc gcaacgaatt agaacgccaa ttgccgggcg taaaagtgac caccaacatc
     9061 gtgagcttct ttggcggggc cgaccaggcg cctattcagg tcatcgtcag tgcgggacgt
     9121 ttggaagacg ctatgaaata tgccaaattc ctgctcgaaa aagccaaaaa agtgcccggc
     9181 accctcgaag cggaaatcac cgtgaaagaa ggcaacccgg aaatcaacgt ggccatcaat
     9241 cgcgaacgca tggccgaact gggcctcgat atgcagatgg tgggcgctac catgcaaacg
     9301 gccttttcag gcaataccaa cgccaaatac cgcgacggcg ccaacgagta cgacatcaat
     9361 gtgcgcttgg acgccttcga tcgccgcaac ctcgccgata tcagccgcct cactttcatc
     9421 aacatgcgcg gcgagctgat ccgcttggac caatttgccg ccgtgaccca aaccaccggc
     9481 acctcgcagt tggaacgcaa agaccgcacc gccgccgtca ccgtacaatc caaagtattg
     9541 ggccgcccat cgggtaccgt cggcgctgaa atacaggaaa ttattgccca aaacccgccg
     9601 cccgccggcg tttccatcgc ttacgacggc gacctcaaag cgcaggaaga aggcttcggg
     9661 agtttgggac tggctttctt agcggccatc ctctttgtgt atctcatcat ggtggcgctg
     9721 tacgacaact gggtgtatcc ttttgtagtg ttgttctcca ttccggtagc catcgtgggc
     9781 gccttgctgg ctatggcatt ggccatgcaa acgctcgaca ttttctccat cgtgggcatc
     9841 atcatgctgg tgggtttggt gggcaaaaac gccatcctct tagtggactt tgccaaccag
     9901 atgaaagcag aaggattcgg cacgttcgac tcgctgattc tggccggtcg catccgtttg
     9961 cgccccatcc tgatgaccac catcgctatg gtattcggta tgatgcccat tgcattggcc
    10021 aaaggcgccg gcgcggagtg gaaaaacggt ctggcctggg ctttgatcgg cggcctcacc
    10081 agttccatgt tgctcacact cgtggtggtg cccgtcattt attacatcat ggactggatc
    10141 aagggcgacc tgaaacgcca aaagcgcaac ggggtaaggc cgattgagga gtaccacggc
    10201 aacgacgatc atggcacggg ggtgcatttt gagggtagtt gatgtgacga tgtgctgatg
    10261 tgacaatgtg ctgattgcca actaagagcg ttcaaaaaag tatcgaatca gtttatttga
    10321 ttccccaagc gggaaatctg acatttaccg ataaccaagc tcacagctcg cggctcaaag
    10381 ctcgcagctc tgttccaaga gagataatat actcaactgc acataaatcg gatactgtct
    10441 cacgggttgt cgaaagacgc ttgtggggca gttttttttg aagttatgcg gccacttcta
    10501 agcgaaggtc catggctttc agtttaaaaa acatcccctc ttttttgcgt ttcacctcca
    10561 atttttgcat cgcacctgca caaaaccacc tcaatatgac ggagcaagac atagaccgca
    10621 tcatagaaat ggcatgggaa gaccgcaccc cttttgaggc cattgagttg cagttcg
//
LOCUS       DAORLF010000117        10628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3349_length_10628_cov_2.166462, whole genome shotgun sequence.
ACCESSION   DAORLF010000117 DAORLF010000000
VERSION     DAORLF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_10628_cov_2.166462"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..>754)
                     /locus_tag="PK198_10945"
     CDS             complement(437..>754)
                     /locus_tag="PK198_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39299.1"
                     /translation="EGQSLTIPITIPVLLAFYIMMAAVQAPNSSMAVWSSIFPLFSPI
                     VMPARLAFGPPLWEIALSMVLLAAASVFFVWLSGRIYRVGILMYGKKVTLKELGKWMF
                     YKE"
     assembly_gap    755..811
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<812..1761)
                     /locus_tag="PK198_10950"
     CDS             complement(<812..1761)
                     /locus_tag="PK198_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39300.1"
                     /translation="MKKLGIIIRREYLTRVTRRSFIIATFLTPLGFALLFVVVGVIFS
                     YKSDDVSKVAILDHDNILGGSIKDEQNLFFKFEDKSLDEMRNTLPDTDYDGILVLPPL
                     KDVLAGRYTGFYYSEKQPAPDIEMAIKNRLGEAIRDYKIKSLQLDPKQLAALDTRIEL
                     DPEPLKQGGQDASVMTSAIASGIGFLMGIIMYMAVFIYGMMIMRSVMEEKTTRIVEVM
                     ISSVRPFELMLGKIIGVGAVGLTQFAIWAILIPLISMGASLAFGMDASAMNASANMNA
                     AEINPDDMQAMIGLAMHEINSINWWFMIPAFVFYFLGGDF"
     gene            complement(1758..1859)
                     /locus_tag="PK198_10955"
     CDS             complement(1758..1859)
                     /locus_tag="PK198_10955"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39301.1"
                     /translation="MDKGLEIHAFNEILPSLNEIFIGRVGASNEAAQ"
     assembly_gap    1883..1902
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1903..2691)
                     /locus_tag="PK198_10960"
     CDS             complement(<1903..2691)
                     /locus_tag="PK198_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39302.1"
                     /translation="MAENILELDGVLKTYGNYTAVNRVSFEVPKGSIFGLLGPNGAGK
                     TSLIRIITTITRADAGSVRLDGVPLNSRHPDYIGYMPEERGLYKKMKVGEHLLYLGQL
                     KGLDKAAAQKELAYWFEKFEIRDWWNKKVEELSKGMQQKIQFIATVLHRPKLLILDEP
                     FSGLDPINTNLIKDEIEELRQKGVSILFSTHRMEQVEEICENIVLINKGSVILNGSVK
                     DIKNQFKENLFRIDFEGELPSDFASQAAVTDFKPGYVTVKMAEGQ"
     gene            complement(2816..>3519)
                     /locus_tag="PK198_10965"
     CDS             complement(2816..>3519)
                     /locus_tag="PK198_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HRF39303.1"
                     /translation="EQGIIYRLDPATGGILQRINFADDGDFEGVAAVGDTVYAIKSTG
                     TLHRITFAPDGTATALKFKSHIDKTYDVEGLTAVSQNRQLLMACKAAPPQLPQGEKHV
                     YAWNLGNMTMSEQPALVLRLSDMLDFLQRNPATPRQEKILEYLNRAEPPGSLRFSPSG
                     IAVHPSLEHWYILSGPGRLLAVYSPKGQLLALHRFDKDLLEQPEGICFDPQGNLYIAS
                     EGKEGPAVLCIWKMR"
     assembly_gap    3520..3544
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3545..3733)
                     /locus_tag="PK198_10970"
     CDS             complement(<3545..3733)
                     /locus_tag="PK198_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39304.1"
                     /translation="MQKLNASRLLLLCLALWCCSGCRTTAQNAALPYHLDKPERRTEL
                     APELLEISGLEWAPGEKYL"
     assembly_gap    3830..3845
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4056..4247
                     /locus_tag="PK198_10975"
     CDS             4056..4247
                     /locus_tag="PK198_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HRF39305.1"
                     /translation="MAYNWQQADWPHFSYDASKFEQAASVFIQKSAYAQGVLDGTEAA
                     GQEQSILDMLVREEAPGIR"
     gene            complement(4304..5779)
                     /locus_tag="PK198_10980"
     CDS             complement(4304..5779)
                     /locus_tag="PK198_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRF39306.1"
                     /translation="MNLTIENILLVGSILLLVSIIAGKTSYKFGVPTLILFLGIGMLA
                     GSDGIGGIQFNDPKIAQFIGVVSLNFILFSGGLDTNWNSVKPILREGVILSTLGVIMT
                     AVSLGMFVWFITDFTFYESMLLGSIVSSTDAAAVFSVLRSKSLALKHNIRPTLELESG
                     SNDPVAYVLTISFLSLVVHQDMSAWSIIPLFLRQMVIGTLLGFAFGHLSKIVINKIKL
                     DFEGLYPVLVIALMFITFSATDFIGGNGFLAIYICSVYLGNQNLIHKKSILKMFDGLA
                     WLMQIVLFLTLGLLVFPTQMTPYIGIGLLISLFLMLVARPLGVLLSLSPIRMKMRKRL
                     YISWVGLRGAVPIVFATYPMLAGIEKADMIFNIVFFVSVTSVLIQGTTLTAVAKWLHV
                     GLPEKVKPQSPTDAFLAEMPKAIMKEIRLSDTCTAVGKKIVELNFPKSAIIAMIQREG
                     KYITPNGATELETEDVLIVLSDTQEGISKVFDIFQPEAGSN"
     gene            complement(5914..6372)
                     /locus_tag="PK198_10985"
     CDS             complement(5914..6372)
                     /locus_tag="PK198_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39307.1"
                     /translation="MEMGTIITGVIIIILCILPFVLLGSGRRKRQKQLLQSLQAAAAQ
                     QGGKIARHDCMRDAAIGIDDEARVLYFIKNDAAQHIRLQSIKSCRSVNNARTVDNNRV
                     IDQLALALLPAGNGQPEIQLEFYNQETDVQLTGELQLLEKWAQIVGAALK"
     gene            complement(6495..6875)
                     /locus_tag="PK198_10990"
     CDS             complement(6495..6875)
                     /locus_tag="PK198_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39308.1"
                     /translation="MKSPIKQIASFSLALLYVFVALPASSAALRAEQPPLGDTGHTRG
                     MQFSVVSGDWLYSLPQSSTFTLPARQSDAPASTKLLLHGLSAVEQSEALAGAVLLACY
                     LRQTAGFPVHLRKSDLIFPFHYFW"
     gene            complement(6885..7457)
                     /locus_tag="PK198_10995"
     CDS             complement(6885..7457)
                     /locus_tag="PK198_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF39309.1"
                     /translation="MEITEVFYPAHRAEWRAWLEAHHTVKNEIWLRTFLKASGKPSLP
                     YDDMVEECLCFGWIDGIAKKYDEESAVRRITPRRKKSFLSELNRQRIWKLQDQGLMTP
                     AGVAPIADQLGSPDDPFEIPGWVEEQLRADPAVWHTFQNFPRLYRRLKIGWIAEVRGN
                     SRRAEAQKRLNYLIKMTAQGKMYGTVPIKE"
     gene            complement(7483..8166)
                     /locus_tag="PK198_11000"
     CDS             complement(7483..8166)
                     /locus_tag="PK198_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRF39310.1"
                     /translation="MKYIIVGLGNFGASLAEKLTLQGHEVIGIDHSMSKVDMYKEKVS
                     HTICLDAMDEFTVSGLPLKDTDVVIVAIGEDQGANVMTTAVFKNLQVKRLISRAINPL
                     HEKVLRAIGVDEIVHPEEETAERWAKKLCLSNVVDSFELSDDYSIIEVSVPPVFIGKT
                     IQEIGLRRKYNLLALTTIKNREVKSTVGRIQNKSVVQGVASPDTVLEKGDILVIYGSK
                     RDLQTFTNQ"
     gene            complement(8177..9928)
                     /locus_tag="PK198_11005"
     CDS             complement(8177..9928)
                     /locus_tag="PK198_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRF39311.1"
                     /translation="MRLKKHYLYSIALVASIIGMIAFILDFGFVQSEFEQQLIDGFYF
                     LVIAIGLLSTFARYFKKPDFSRKKVFIFDLLSVLYTLYIFYMYLFVGEAFTTDLILEN
                     PLWVIMAVILTFFREFFDRKINFSRAFLNPAQLFILSFFLVILFGSFLLMLPNAANEK
                     ISFLDALFTSTSAVCVTGLIVVDTATYFTTFGKVIILFLIQIGGLGILTFASYFSYFF
                     KGVSTYESQLTLSEMTNSGKIGEVFKTLKYIILITLTIEGISALMIFLTLRSAPFETF
                     TDRVFFSVFHAVSAFCNAGFSTLTNNLYEDGFRFNYALQLIVIITLVFGGLGFPIVAN
                     MLEFVKHKVRLILSNTPNTKTHRPWVLNINSRITLITTIVLIVTGFVLYFLFEYNHTL
                     SNHTSLWGKIVTALFGSTTPRTAGFNTVDMTALSFPTVMITFLLMWIGASPASTGGGI
                     KTSTFAIATLNILSLAKGKNRIEIYRREISDISVRRAFATIALSLMVIGAGITIITVS
                     DPEKGLLNIGFECFSAYSTVGLSLGITAGLSNTGKLIIIAIMFIGRVSMLSIVIAFFK
                     KVKHKNYRYPTEEITIN"
     gene            complement(10016..>10628)
                     /locus_tag="PK198_11010"
     CDS             complement(10016..>10628)
                     /locus_tag="PK198_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF39312.1"
                     /translation="VADKVLDDTYTTALAGQSKDFKESSSSLLFAFGLALILIFLVLA
                     AQFESFRDPFIILFTVPLAVAGAVTALWYFDATLNIFSQIGIIMLVGLVAKNGILIVE
                     FANQRKEQGLSRLDAIKDASVSRFRPILMTSLSTILGTLPIALALGSGSGSRISMGIV
                     VVAGMAFSTLLTLYVIPAIYSYLSSKEPNKIVIIEEEAEVMVA"
BASE COUNT         2738 a         2540 c         2638 g         2594 t
ORIGIN      
        1 attcctgatt tcggattcct gatttcggat tcccgatttc gaattcccga tttcgaattt
       61 cgggggtagt gaaaaggcga ctctgcatgt tttagccccg cagtcacccc ttccctaagc
      121 tggagaaaac cgcccccgca agtcagggcg caaccggaag ttacggcctg actccgacgc
      181 tgaaagacgc ggattcttat gatcgtttgt gccccctctc cgagcagcgg aaatcataaa
      241 aaccataaag atcagcgtca aaaaacaaag cgtggttttc gcgttccggt tttcggcttg
      301 cagataaaca actgcgaagc agattactac gaagtagatg aactacgaac cgcgcctcgc
      361 gcgggaagca gattactgcg cagcggataa actgcgcagc agcttactgc cgaaggcaga
      421 taaactgcgc agcagattac tccttataaa acatccactt gcccagttcc ttcagcgtca
      481 cctttttgcc atacatcagg atgcctacac ggtaaatgcg ccccgacaac cacacaaaga
      541 acaccgatgc ggcagccagc aataccatag acaaggcgat ttcccatagc ggcggcccga
      601 acgccaaacg cgccggcatg acgatgggcg aaaacagggg gaagatagac gaccacaccg
      661 ccatactcga attgggggcc tgcacggcag ccatcatgat gtaaaatgcc agcaataccg
      721 gtatggtgat gggaatagtg agcgattgcc cttcnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaagtcc ccgccgagga agtaaaatac
      841 aaatgcaggt atcataaacc accagttgat gctgttgatc tcgtgcatgg ccaacccgat
      901 catggcttgc atgtcgtcgg ggtttatttc agcggcgttc atgttggccg aggcgttcat
      961 agcagatgca tccatgccga atgcaaggct ggcgcccata ctgatgagcg gaatgagaat
     1021 ggcccagatg gcaaattgcg taagccccac cgcacccacg ccgatgatct tgcccagcat
     1081 gagttcaaag ggccgcacgg aggaaatcat cacctctaca atgcgggtgg ttttttcctc
     1141 catcacactg cgcataatca tcataccgta aataaataca gccatgtaca tgatgatgcc
     1201 catgagaaaa ccaatacccg atgcaatggc actggtcatc accgaggcgt cttgcccgcc
     1261 ctgcttcagc ggctcggggt cgagttcaat gcgggtatcc aaggctgcca attgtttggg
     1321 atccaattgc agacttttga ttttgtaatc gcgtatcgcc tcgccaagac ggtttttaat
     1381 ggccatttcg atgtcgggcg cgggctgttt ttctgaatag taaaacccgg tatagcggcc
     1441 tgcaagcacg tccttgagcg gcggcaatac gaggatgcca tcgtagtcgg tgtcgggcag
     1501 ggtgttgcgc atttcgtcga gggatttgtc ttcaaatttg aaaaacaagt tttgctcgtc
     1561 tttaatgctg ccgccgagga tgttgtcgtg gtccagaatg gccaccttgc tcacatcgtc
     1621 gcttttgtaa gaaaaaatga cgcccactac cacaaaaagc agggcgaaac caagtggcgt
     1681 gagaaaagta gcgatgatga aggagcggcg cgtaacgcgg gtaaggtatt cgcgccggat
     1741 gatgatgccg agttttttca ttgtgctgct tcgtttgagg cgcccactct gccgatgaag
     1801 atttcgttga gggagggcag gatttcgttg aaagcgtgaa tttcgaggcc tttgtccaaa
     1861 aagaatttga gcagggcatt ggnnnnnnnn nnnnnnnnnn nnctggccct ccgccatttt
     1921 tacggtcaca tagccgggct tgaagtcggt gacggcggcc tgcgatgcga aatcagaggg
     1981 cagctcgcct tcaaaatcaa tgcgaaagag attttctttg aactgatttt taatgtcttt
     2041 cacggagccg ttgagaatta ccgagccttt gttgatgagg acgatgtttt cacaaatttc
     2101 ctccacctgc tccatgcggt gcgtcgaaaa gaggatgctc acgccctttt ggcgcaattc
     2161 ctcaatttcg tctttgatga ggttggtatt gatggggtcg aggccggaaa atggttcatc
     2221 cagaatgagc aatttgggcc ggtgcaatac agtggcgata aactgtattt tttgttgcat
     2281 ccccttcgac agctcttcca cttttttatt ccaccagtcg cggatttcaa acttctcaaa
     2341 ccagtaagcc agttcctttt gagcggcggc tttgtccaag cctttcagtt gaccgaggta
     2401 gagcaaatgc tcgcccacct tcattttttt gtagaggccg cgctcttccg gcatgtagcc
     2461 gatgtagtcc gggtggcggc tgttgagtgg cactccgtct aatctcactg aaccggcatc
     2521 ggctctggta atggtggtga tgatgcggat gagcgaggtt ttgccggcgc cgttggggcc
     2581 taaaaggcca aaaatactgc ctttgggcac ctcgaaactg acgcgattga cggcagtgta
     2641 attgccgtag gttttcagca cgccgtctaa ttctagaatg ttttctgcca taatcgtccc
     2701 tgagttgtga ttttggttgc taaagtaaca aaagccatcg cctggccggc ccgattttct
     2761 gatggagggc tgttttttct atatatacga ctgccttcgg gcgaagaatg accacttacc
     2821 tcattttcca gatgcacaat acggcgggac cttctttgcc ctcgcttgcg atgtagagat
     2881 tgccctgtgg gtcgaaacaa atgccttcgg gctgctccag caggtcttta tcgaagcgat
     2941 gcagcgccag caattgccct ttcggggaat acacggccag caggcgaccc ggcccggaaa
     3001 ggatgtacca gtgctccagc gagggatgta cggcaatgcc cgatggactg aaacgcaggc
     3061 tgcccggagg ctcggcgcgg ttcaggtatt cgagtatttt ctcttggcgg ggcgtggctg
     3121 ggttgcgttg gaggaagtcg agcatatcgc tgagccggag aacgagggct ggctgttcgg
     3181 acatagtcat atttcccaaa ttccaggcat acacatgctt ttcgccttgc ggcaactgag
     3241 gcggcgcagc tttgcaggcc atgagcaatt gtcggttttg ggaaacggcg gtgaggccct
     3301 ccacgtcgta ggttttgtca atatggcttt tgaacttgag cgcagttgcc gtaccatcgg
     3361 gggcaaatgt gatgcgatgc aaagtgcccg tactcttgat ggcgtacacc gtgtcgccca
     3421 ctgcggccac tccttcaaag tcgccgtcgt cggcaaaatt tatgcgttgc agtatgccgc
     3481 cggttgcagg gtcaaggcgg tagatgatgc cctgctcatn nnnnnnnnnn nnnnnnnnnn
     3541 nnnngaggta tttctcgccg ggcgcccact ccaaaccgga gatttccagc agttcgggcg
     3601 cgagttccgt gcggcgttcg ggcttgtcca agtggtaagg caatgcagca ttttgggccg
     3661 tggtgcggca tccggagcag caccagaggg cgaggcagag gaggagcaga cgggaagcgt
     3721 tcaacttttg catcaggcaa aggtagggaa aaaaatcccc tccaacatat tccatggact
     3781 gaccgtagcg gcgctccgaa tccgggcatc ccggcctccg tagataagcn nnnnnnnnnn
     3841 nnnnnccggg cgtgaacggg tgtgccggcg ggcgcatgtt tttgaaaaaa gaggtttagt
     3901 gtacaaacac agctacgacc actcttatgc aaatataaaa ttcaattttc gatttgcata
     3961 aaagtagaaa aagttgaacc acctgagccg attactcatg ctaatcggct caaaaaagga
     4021 gtatatttga gccgattaat acaaaaaatc ggctcatggc ttacaattgg caacaggcag
     4081 actggccgca tttttcctac gatgcctcaa agttcgagca ggcagcatcc gtttttatcc
     4141 aaaaatccgc ttatgcacag ggcgttctgg atggaacgga ggcggccggg caagagcagt
     4201 ccatacttga tatgctggtg cgggaagaag caccgggtat caggtgaatc tggatgcctg
     4261 aggcgggtgg gcagttttta caaatgctga gttgtggaat attctaattt gaacccgctt
     4321 ccggctggaa gatgtcgaac accttgctga tcccttcctg cgtatcggaa agcacgatga
     4381 gtacatcttc ggtttccagt tcggtagccc cattgggcgt aatatacttc ccctctcttt
     4441 ggatcatggc aatgatcgca gattttggaa aattcaactc tacaattttt ttcccgacgg
     4501 cagtgcaagt atcactcagt ctgatttctt tcatgatggc tttgggcatt tctgccagaa
     4561 acgcatcggt tgggctttgc ggtttcacct tttcaggcag ccctacatgc agccactttg
     4621 ccaccgccgt cagggtagtg ccctgaatca atacggaagt gacggaaacg aaaaatacaa
     4681 tgttgaaaat catgtcggct ttttcgatgc ctgccaacat agggtacgtt gcaaaaacga
     4741 tgggaacggc gccccgcagg cctacccagg agatgtacaa tcgtttccgc attttcatcc
     4801 gtatgggcga caagctcaat agtactccta aaggccgcgc caccaacatt aaaaacagag
     4861 aaatgagtag ccctatgccg atgtatggcg tcatttgggt agggaatacc agcaatccca
     4921 aagtaaggaa caatacaatc tgcatgagcc aggccaaacc gtcgaacatt ttcagaatgg
     4981 attttttgtg tatcaggttt tgattgccca aatacacaga acagatgtaa atggccagga
     5041 atccattgcc tccgatgaaa tcggttgctg aaaaagtaat gaacatcaaa gcgattacca
     5101 aaacagggta caagccttca aaatcgagtt tgattttatt gatcacaatt ttactcaaat
     5161 gcccgaatgc aaaacccagt aaagtgccta tcaccatctg gcggagaaat aaaggaatga
     5221 tagaccaggc gctcatatcc tggtggacca cgaggctgag aaacgagatc gtgagcacat
     5281 acgccacggg gtcgttgctg ccgctttcga gttccaaggt ggggcgaatg ttgtgcttga
     5341 gtgccaggct tttagaacgc aatacggaaa aaaccgcagc ggcgtcggta gaagacacaa
     5401 tggagccgag taacatactt tcgtaaaaag tgaagtctgt tataaaccaa acaaacatac
     5461 ccagcgaaac cgccgtcata atcactccaa gcgtagaaag aatgacgccc tcccgcaata
     5521 tgggctttac ggagttccag ttggtatcta agccgcctga aaacaaaatg aaattcaatg
     5581 ataccacccc gatgaactgg gcaattttag ggtcattgaa ttgaatgccg ccaataccat
     5641 cagaaccggc caacatacct atgcccaaaa aaaggatcaa ggtaggcacc ccgaatttat
     5701 aggatgtctt gcccgctatg atgctcacca agagcagaat agaccccacc aataggatat
     5761 tctcaatcgt cagattcatc tcttttttgt attcatccgg cacaaaagaa tcaataatta
     5821 tctatgtagt ggccaaagat aaactataca catctgaata ccaaaaagcc ccgcaggtga
     5881 tctgcgaggc tcgtctttgg ttgtatttgg gagttatttc aaggctgccc ccacgatctg
     5941 cgcccatttc tccaagagtt gaagctctcc agtgagctgg acatcggttt cctgattata
     6001 aaattccagt tgtatttccg gttgtccgtt gccggcaggc aggagcgcca acgcaagctg
     6061 atctatgacc cggttgttgt ccacagtacg ggcattgttg acggagcggc aagatttgat
     6121 gctttgcagg cggatgtgct gagcagcatc attttttata aaatacaaaa cacgcgcttc
     6181 atcgtctatc ccgatggcgg catcgcgcat acaatcgtgg cgggcgatct tgccgccctg
     6241 ctgtgctgct gccgcctgga gcgattgcaa aagttgtttc tggcgtttgc gccggccgct
     6301 gcctaataat acaaaaggta ggatacaaag aatgatgatg ataaccccgg tgatgatggt
     6361 tcccatttcc atgatgatga ttttttttgg atggaatatg ctcaataatc agcatcggcc
     6421 cgacggaggc gccaggcggg tacgaacagc gagattttga ggatgctata caaaatcgct
     6481 gttgctgaag agcattacca aaaataatgg aaagggaaaa tgaggtcgga ttttcgcaaa
     6541 tgcaccggaa agccggcagt ctggcgaagg taacaggcca gtaaaactgc gccggccaat
     6601 gcttccgact gctccacggc agacaagcca tgcagcagca atttggtgct tgcaggagca
     6661 tcgctttgcc gggccggcag ggtgaaggtg cttgattgtg ggaggctgta cagccagtcg
     6721 cccgaaacca ccgaaaactg catccccctc gtgtggccgg tatcgcccaa cggcggctgc
     6781 tcggcgcgca aggctgcgct gcttgcgggc aatgccacaa agacgtagag caatgccaac
     6841 gaaaaggaag cgatttgttt gattggtgat ttcacggcgc aaagttattc tttgatcgga
     6901 acagtgccgt acatttttcc ctgcgccgtc attttgatga ggtagttcaa tcgtttttgc
     6961 gcctcggcgc ggcggctgtt gcctctgact tctgctatcc agcctatttt caggcggcgg
     7021 tacaggcgcg ggaagttttg gaaagtatgc catacggcag gatcggcccg gagttgttct
     7081 tccacccagc cggggatttc aaacgggtcg tcgggcgagc cgagttgatc ggcaatgggg
     7141 gctacgccgg cgggcgtcat caggccctga tcttgcagtt tccatattcg ctgccggttg
     7201 agttctgaca aaaaactctt ttttctgcgc ggtgtgatgc gccgtaccgc gctttcttcg
     7261 tcgtattttt tagcaatgcc gtcaatccag ccgaaacaaa ggcactcttc caccatatcg
     7321 tcatacggca gagatggctt tccgcttgcc ttgaggaagg tgcgcagcca tatctcgttt
     7381 ttgacggtat gatgcgcttc gagccaggca cgccattcgg cccggtgggc agggtaaaat
     7441 acttcggtga tttccatagc tgtattttgc ggtcccggcg gcttattggt tcgtaaatgt
     7501 ttgcaaatct ctcttggatc cgtaaatgac aagaatatct cccttctcaa gaacagtgtc
     7561 cggcgatgcg acgccttgaa caacagattt gttttgaatc ctgcccactg tgcttttcac
     7621 ttctctgttt ttaatggtag tgagcgccaa aaggttgtac tttcttcgga ggccgatctc
     7681 ctgaatcgtt ttccctataa aaaccggtgg tacgctgacc tcaataatac tgtaatcatc
     7741 gctgagttca aaggaatcca ccacattgga caaacacagt ttttttgccc agcgctcggc
     7801 tgtttcctct tcgggatgaa caatttcatc cacaccaata gcccgcagca ctttttcgtg
     7861 aagcgggttg atggctctgc tgatgaggcg ttttacctgc aagtttttga ataccgctgt
     7921 ggtcatcacg ttggcgcctt gatcttcacc aatagcaaca atgactacat ctgtatcttt
     7981 caaaggtaaa cctgatacag taaactcgtc catggcgtcc aagcaaatgg tatgtgatac
     8041 cttttctttg tacatatcca cttttgacat actgtgatca atgccaatca cttcatgacc
     8101 ttgcagcgtc agtttttcgg cgagggaagc gccaaaattt ccaagcccga caatgatgta
     8161 tttcatgttc ttctcattaa ttgatagtga tttcttcggt ggggtatctg taatttttgt
     8221 gtttcacttt tttgaaaaaa gcaataacaa tagataacat actgaccctg ccgataaaca
     8281 taatagcaat aataatcaat ttgcctgtat tgctcagacc tgccgtgatg cccaggctca
     8341 agcctaccgt gctgtaggcc gaaaagcatt caaaaccaat attcaaaagc cctttttcgg
     8401 gatcagagac cgtaataata gtaattcccg ctccgatcac catcaatgac agcgcaatgg
     8461 ttgcaaacgc ccttctgaca gatatgtcgg agatttcccg tctgtaaatc tctatgcggt
     8521 ttttcccttt ggccaaactt aatatattga gcgtggcaat agcaaatgta ctcgttttga
     8581 tacctccgcc tgtggatgcc ggagaggcgc ctatccacat gagcaaaaaa gtaatcatga
     8641 ccgtaggaaa actcaatgca gtcatatcca ccgtattgaa cccggcggtt cgcggcgtcg
     8701 ttgagccgaa aagtgcggtc actatcttac cccaaaggga cgtatgattg gaaagcgtat
     8761 ggttgtactc gaaaaggaag tataaaacga agcctgtcac aatcaatacg atggtggtga
     8821 tgagtgtgat tctgctgttg atattgagta cccaggggcg gtgggttttg gtattggggg
     8881 tgtttgaaag aattaaacga actttgtgtt taacaaactc caacatgttg gccactatcg
     8941 ggaaccccag gccgccgaaa accaaagtaa taatcacaat caactgtagt gcataattga
     9001 acctgaatcc gtcttcatac agattgttcg tcagcgtgga aaaaccggca ttgcaaaagg
     9061 cggaaacagc gtgaaaaacc gagaaaaaca ccctgtctgt aaatgtttca aaaggtgcgc
     9121 tcctgagggt gagaaaaatc atcaatgccg atattccctc aatggtcagg gtaatcagaa
     9181 tgatgtattt cagcgtttta aacacttccc ctatttttcc ggaattggtc atttcgctca
     9241 gcgtcaactg gctttcatag gtggaaactc ctttgaaaaa gtagctgaaa tagctggcaa
     9301 atgtcaaaat gcccaagccg cctatttgta tcaaaaacaa tatgataact ttgccgaaag
     9361 tggtaaagta ggtggctgta tccacgacga tcaaaccagt aacgcacacc gcactggtag
     9421 aagtaaacag ggcgtcaaga aaggatattt tttcattggc ggcattgggc agcatcaaca
     9481 gaaaactgcc gaacagaatg acaagaaaaa aactcaaaat gaacaactgg gccggattga
     9541 gaaatgccct gctgaaattg atttttcggt cgaaaaactc gcgaaaaaaa gtcaggatga
     9601 cggccataat tacccataga gggttttcga ggatcaggtc ggtcgtaaaa gcctctccta
     9661 caaataaata catgtaaaaa atatacagag tgtagagtac cgacagtaaa tcgaagataa
     9721 ataccttctt gcgggagaaa tccggtttct tgaagtacct cgcaaaggtt gacaacaggc
     9781 cgatggcgat aaccaaaaag taaaacccgt ctataagttg ctgctcgaat tctgattgaa
     9841 caaatccaaa atccaaaata aatgcgatca tccctatgat gctcgccacc aaagcgatgc
     9901 tgtagagata gtgttttttc agtctcattt tatgcaagta gctgatacct taatgttgat
     9961 acttctttgg aaattattga ctcattagta tcaaaatcct gtttcattct tgcaatcaag
    10021 ctaccatcac ctccgcctct tcctcaatga tcacgatctt atttggctcc ttgctggaca
    10081 gataagaata aatggccgga atcacataca aggtcagcag cgtagaaaat gccatacctg
    10141 ccaccaccac gatacccatc gaaatgcggc tgcccgagcc tgaacccagc gccagtgcaa
    10201 tgggcagcgt acccaaaatg gtagaaaggc tggtcatcaa aatgggccgg aaacgagaca
    10261 ccgatgcgtc tttgatagcg tctaaacggc tcagtccctg ctctttgcgc tggttggcaa
    10321 actctacgat gaggatgccg tttttggcca ccaaacccac cagcatgatg atgccgatct
    10381 ggctgaagat gttgagcgta gcgtcgaaat accacaacgc tgtcaccgcg ccggctacgg
    10441 ccaaaggtac cgtaaagagg atgatgaacg gatcgcgaaa actctcgaac tgcgccgcca
    10501 ataccaaaaa gatgaggatg agcgccagac caaaggcaaa caacaaactg gaagagcttt
    10561 ctttgaagtc tttcgactgc ccggccaatg cggtggtgta tgtgtcgtcc aatactttat
    10621 cggctaca
//
LOCUS       DAORLF010000118        10580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3364_length_10580_cov_2.061188, whole genome shotgun sequence.
ACCESSION   DAORLF010000118 DAORLF010000000
VERSION     DAORLF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_10580_cov_2.061188"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>261
                     /locus_tag="PK198_11015"
     CDS             <1..>261
                     /locus_tag="PK198_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRF39313.1"
                     /translation="SNELGSGLKMSSGPVIEKPGDLAGLLTNLDAGDVLFIDEIHRLN
                     TVVEEYLYSAMEDYRIDIMIDSGPNARSIQISLNPFTLIGAT"
     assembly_gap    262..282
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <283..813
                     /locus_tag="PK198_11020"
     CDS             <283..813
                     /locus_tag="PK198_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRF39314.1"
                     /translation="APMRARFGITCHLDYYDAATLLRIIKRSAGILGVEITEEGALEI
                     ARRSRGTPRIANALLRRIRDFAQIKGSGTIDVAIAQFGLAALNVDEGGLDEMDNRILS
                     AIIHKFKGGPVGLTTIATAVGEEPGTIEEVHEPFLIMEGYIQRTPRGREATEKAYRHL
                     GVVPPGRAGTLFELEM"
     gene            complement(1430..2773)
                     /locus_tag="PK198_11025"
     CDS             complement(1430..2773)
                     /locus_tag="PK198_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39315.1"
                     /translation="MHTKSIVLPSIIEDFENALAESMPFSGNSEDYLAIISHYRQSDF
                     QSLALEAAETAARQFPDHLQFFLIQAQLLIEMRRETQAIELLNVAYTVDHQHVSIELM
                     RAEALTCMGRYDAALELIEALKNQSPETQLADILVAEAMVYEQRQEHERAFLVLKTAI
                     ELHPGHQRVLERLGVCVENTRRYEESIILHGELIDAAPYAFQAWYNLANAFAGTGRYE
                     EALQAYEYAFLTNEDFEQAYYDYAELSLELGKPERALQCYEEAASRFELGADQFIQLG
                     RCHEALGNYQDAATAYIKALGYEPFDDELLYHIGCCQMAMCQFDKAARYFRRALSIDD
                     AGEEYHLALAEALSAGAKYAKAEFHFHEALCLSQYEERYLLAYARFLLIQGRALEALT
                     LLGEAFDETEASPRLVYAHIVCLKAVGRQAEASYRLAEAMERFPEEASFLTDWAA"
     gene            complement(2956..4503)
                     /locus_tag="PK198_11030"
     CDS             complement(2956..4503)
                     /locus_tag="PK198_11030"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39316.1"
                     /translation="MATPPLPIGIQDFRKIRENGFKYIDKTMYLYELCQSPGTYFLSR
                     PRRFGKSITLATLHELYSGSRELFKGLWIEDKWDWGKKHPVIRISFKDINFEQRGLES
                     PLAERMKEIAAQYGVELKTPTARDRFRELATVLAATAKVVVLIDEYDAPITHYLGKEL
                     PQAIENRDFLRGFYSVLKEADHLLEFVFLTGVSKFSKVGIFSGINNLQDLTIHPAYST
                     MLGYTQVELESNFSEEITKTAAQMGLGREALLEQMHLWYNGYRFHPDAERVYNPVSVN
                     LFFDVRDFQNFWFATGTPTFLVQLLKKEGLFDLHFPAMNLNGFESFDLERLKPVSILF
                     QTGYLTIQGKDEDGLVLLDYPNKEVRDSMLEVLIEGFVGVDAEFSSAFIIKIRNAFRA
                     NDLEALIRILQGVFKKLPYFLHEHYPEKFFHAAIHLLFSLLGVRIRSEVCTSDGRVDS
                     VVETDTHVYILEYKLDESAEAALAQIRRKQYYQAFWDLGKPVIGVGVNFSSASKNIDD
                     WKAEVMS"
     gene            complement(4546..5304)
                     /locus_tag="PK198_11035"
     CDS             complement(4546..5304)
                     /locus_tag="PK198_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39317.1"
                     /translation="MKARFLIPVLAVLLAASCGDNKTDDGSVSLQFKADFGGAPLSMF
                     TRSYAYPEGMNVKFQNFLFYVSDVTLIKEDGTEHALSEVELISFKNIQDDAAAAAGVV
                     TQAAKVPPGNYKAVKMGIGIAPDLNGTQPGDYKPGHPLTDNYWSWALGYIFTKIEGNA
                     DLNGDGNYDENAKLTFHIGANSLYQVKTFNTNFSVETGEEVKLPFTVDLRKVLVNDAG
                     NFLDFRVTRQDHTNDMNVAQFIIDNLNAAIRTAD"
     gene            complement(5317..>5895)
                     /locus_tag="PK198_11040"
     CDS             complement(5317..>5895)
                     /locus_tag="PK198_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39318.1"
                     /translation="VRLVKADGSETPLSNRIEVQVFTSGGSLQKDSITNDILLVSPAL
                     IGINSVGAFRKGGRFTALRFRIGLNDVAAKVDPESLPSGHPMRPAASGMDWNMNEGFV
                     FQQARFLPETAPNDTLRVAARLPAQRQELELPFDVSVPEGYHLELDIRIDYLSWLRDI
                     SPSTQTPETLLQKWTDRSAASFEVVGVRTVSQ"
     assembly_gap    5896..6030
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6265..6783)
                     /locus_tag="PK198_11045"
     CDS             complement(6265..6783)
                     /locus_tag="PK198_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39319.1"
                     /translation="MDANYFALQRKVQEYKTIIANTEKFREEWKSSLRDRIVKTLKDI
                     SKAVELDAFIEVQAPVLNLEAVVLTLGVEKSGLAQRIDSHIKRDFIKQRGSLIYQQLF
                     NGKIIVLINYPFIESYGQPREPKTIAIYRPEELQPDFFLRHLEEFMSEITQWEDYDDD
                     QPAKKIGFHPGT"
     assembly_gap    7167..7304
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7305..>9261
                     /locus_tag="PK198_11050"
     CDS             <7305..>9261
                     /locus_tag="PK198_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39320.1"
                     /translation="AFRKVLADPARRRTALPAYLLPLGIKALGQAITALLGLNPPAEL
                     QSYIPREWPLPYRPAERAAFHHKVRVVALSDDAEQCTLLARTEDRPEETARIAYNLAD
                     HNENFNPTIDTLRSVFRFPVTLHLIDAEVDQEGVYRPRAFVVEPDFLLDVSAVAGCFR
                     EQAAEPWLFLLDKFKPFSVSKHRIIGNIANFFLDELMTDPEAEFKTVFAKVFRLSPLA
                     FCLLPDAEVKEIHQSVQKHFVTLRTMVLRDFAARDIAPEHCFLEPSFYSETYGLQGRL
                     DVFCYQPDDESKAAIVELKSGKPFNTNGYRINHDHFVQTLLYDLMIQSAFDRRLNTAN
                     YILYSSQDEDPLRFAPRIRSQQYDSLQVRNQMAAIERLLQRLGNQQDVDLAEQGRRLF
                     GRISPGRLPALKGFARKDLEAFEAMYFGMTDLERRYFIAFAGFIAREHQLAKTGLIGT
                     DTVNGLAALWLNTTQEKRDGFESLSGLKIARMMARDEEPILELERPATGDVLANFRLG
                     DIAVLYPAQEKGGVLSHQIFKCTIIEISSQKVAVRLRSKQFNDSLFRRFEFWNIEHDL
                     LDTSFVAMYRALYEFAGAPAHRKKLLLGIAAPRQAQPEDIPLSAELTSEQQHILRQAI
                     AAPDYFLLWGPPGTGKTSMMLKHLVSYL"
     assembly_gap    9262..9293
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9294..>9649
                     /locus_tag="PK198_11055"
     CDS             <9294..>9649
                     /locus_tag="PK198_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRF39321.1"
                     /translation="LLLAYTNRAVDEMCEAVESIGPDVRRHYFRIGSRYATHPDFHPQ
                     LLDALIENVGTRRDLKALIDSRRIVLATVASIGGKPEILQLKSFARVIIDEASQILEP
                     MLAGLLPRFERFILIG"
     assembly_gap    9650..9661
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9662..10443
                     /locus_tag="PK198_11060"
     CDS             <9662..10443
                     /locus_tag="PK198_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRF39322.1"
                     /translation="QLPAVVGQSPDASRVEDSALQSIGLHNLRNSLFERLFRLCIQNN
                     YTWAFAQLSRQGRMHRDIMAFPNSFFYQNTLQILPDDTPGALRQTANLPFDAQAARYG
                     LESVVALRRVLFLSTPTDEGGVAQKTNRHEAVLIADLVSAFQRLYDGRLLSIGVITPY
                     RAQIARIRAELESRQIQPDELTIDTVERYQGGARDVILISLCTNSLSALSSLISLSDD
                     GVDRKLNVALTRAREHVVVVGNAELLRENETYAQFIAMYSV"
BASE COUNT         2374 a         2867 c         2575 g         2426 t
ORIGIN      
        1 tttccaatga gttggggtcc ggcctgaaaa tgtcctcagg tccggtcatc gaaaagccgg
       61 gcgacctggc cggcctgctg accaacttag acgccggcga tgtgctcttc attgatgaaa
      121 ttcaccggct caatacggtg gtggaagagt atctctactc tgccatggaa gactaccgca
      181 ttgatattat gattgacagc ggccccaatg cgcgcagcat tcagatttcg ctcaatcctt
      241 tcacgctcat cggcgctacc annnnnnnnn nnnnnnnnnn nngcccccat gcgtgcccgc
      301 ttcggaatta cctgtcactt agactattac gacgcagcca ccctcctgcg tatcatcaag
      361 cgttcggcgg gcatactcgg cgtggaaatc accgaagagg gcgctctgga aatagcccga
      421 cgcagccggg gcactccgcg tattgccaac gccctgttgc gccgcatccg cgactttgca
      481 cagatcaagg gttccggcac catagacgta gccattgctc agttcggcct tgccgcccta
      541 aacgttgacg aaggcggttt ggatgaaatg gacaaccgga ttctcagcgc catcatacac
      601 aaatttaaag gcggaccggt cggactcact accatcgcta ccgcagtggg agaagagccg
      661 ggcaccattg aagaagtaca cgagccgttt ctgattatgg aaggatatat acagcgcacg
      721 ccgcggggcc gcgaggctac tgaaaaagct tatcgccacc tgggagtagt gccgccgggc
      781 agggccggaa cgctgtttga actggaaatg tagttggggt accccctaaa aacagtaagt
      841 cgcaccactc aacgcggtac gacttacttc cgaaaatcat aatcccatga acaattaaaa
      901 ctaaacttac actcccgaag gaggtatatt ttgaggcccg aaggctgaag ttgtcaactt
      961 gataagtttg agaatagcaa gtcggctcgt ggaaaccgac ttgctattga caaacgaaaa
     1021 ttcctatatt ttttgtccaa acttgcttcg ccggctcttt tgcctcgcgt acatctcgtt
     1081 tgacaataca aacatcttac agttttggcc atgatacaag gacataataa agtatttgtg
     1141 aagttaagtt actgcaattt agtgcgagat atttatttta aatatttgtt tatcatggtt
     1201 ttaaaccttt gtaatatttt aaatcgtttc aaattttgtg aaaataactc gtcgtatatt
     1261 catctcatat tggtatcttt tttttgaaaa aaaaatgatt ccccggatca agccggccat
     1321 tttttttcaa aatcgtgctt ttcagcgccc tgttcctgaa acgaatacgg ggccgaccgg
     1381 caaatccgat cagccccgca taagcataga aaagggagaa atttacaact catgctgccc
     1441 aatccgtcaa aaacgacgcc tcttcaggga agcgttccat cgcctctgcc agacggtaag
     1501 aggcttcagc ctgacgtccg acagctttca ggcaaactat gtgcgcataa accaagcgcg
     1561 gagatgcttc cgtttcatca aacgcttcac ccaacaaggt caatgcctcc agggcacgac
     1621 cctgaatcaa taaaaagcgg gcatacgcca gcagataccg ctcttcgtac tgagacaaac
     1681 aaagcgcttc atggaagtga aactcagctt tggcgtattt ggctccggca gacaatgctt
     1741 ccgccaatgc caaatgatac tcttcaccgg catcgtctat actcaaagcc cgccgaaaat
     1801 acctggccgc tttatcaaat tgacacatag ccatctggca gcagcctata tgatacaaaa
     1861 gctcgtcgtc aaaaggctcg tatcccaacg cctttatata tgcagttgcc gcatcctgat
     1921 aatttcccaa tgcttcgtgg caccgcccta attggataaa ttggtccgcg cccaactcaa
     1981 accttgatgc ggcctcctca taacattgca gcgcccgctc tggctttccc aactccagac
     2041 tcaattctgc atagtcataa tatgcctgct caaagtcttc attggtcaga aaagcatatt
     2101 catacgcctg caatgcctcc tcataacggc ccgttccggc aaaggcattg gccagattat
     2161 accatgcctg gaaagcgtag ggggcggcat ctatcagttc gccatgcaaa atgatgctct
     2221 cttcgtaccg gcgggtattc tctacacaaa cacccaatcg ttccaagact cgctgatgac
     2281 cgggatgaag ttcaatcgcc gtttttaata ccaaaaatgc gcgctcatgc tcctgtcgct
     2341 gttcgtacac catagcctct gctacaagaa tatcggcgag ttgcgtttcg ggagattgat
     2401 tttttaatgc ctctatcaac tccaatgcag catcgtatcg tcccatgcag gtaagggctt
     2461 ccgctcgcat cagctctatg gatacatgtt gatggtcaac ggtataagca acattcagca
     2521 gttcgatggc ctgggtttcc cggcgcattt cgatgagcaa ttgagcctga atcagaaaaa
     2581 attgcaggtg gtccgggaat tgacgggcgg cagtttcggc ggcttcgagt gccagcgact
     2641 gaaagtcgga ctgacgataa tgactgataa tggccagata gtcttccgaa ttgcccgaaa
     2701 aaggcatgga ttcggcgagg gcattttcaa agtcctcaat gatggacggg agtacaattg
     2761 atttcgtgtg catagtgttt ccgtgaaatt acgcagtaaa attacaccaa gtcgcggcaa
     2821 ttgtacccat cttatacaga gaagtgtcca accattaagg gaacggtaaa aaaatggggt
     2881 tttttgaatg attcgaggga tgattttttt tgaaaccaac cccaatcact gcctgtttcg
     2941 ataaaaatgc tctcctcaac tcatcacctc cgccttccaa tcatcaatgt ttttggatgc
     3001 gctggagaag ttcaccccca cgccgatgac gggtttgccc aaatcccaaa atgcctgata
     3061 gtattgtttg cggcgtattt gagccaaggc agcctcagcg ctttcatcca atttgtattc
     3121 cagaatatag acgtgggtgt cggtttccac cacgctgtcc acccgcccgt cggaagtgca
     3181 tacctcgctg cggatgcgca cgcccaacag cgagaacaac aagtgtatgg cggcgtggaa
     3241 gaatttttcc gggtaatgct cgtgcaggaa gtagggcagc ttcttgaata cgccttgcag
     3301 aatgcgaata agtgcctcta agtcattggc acggaacgca ttgcgaattt ttatgatgaa
     3361 ggcggaggaa aactcggcgt ccactcccac aaagccttca atgaggactt ccagcatgga
     3421 gtcgcgcacc tctttgttgg ggtagtccag caacacgagg ccgtcttcgt ccttgccctg
     3481 tatagtgagg tagcccgttt ggaagaggat agacaccggt ttgaggcgtt ccagatcgaa
     3541 actctcgaag ccgttgaggt tcatagcagg aaaatgcagg tcgaacagcc cttccttttt
     3601 gagcagttgc accaagaagg taggcgtgcc ggtggcaaac cagaagttct ggaagtcgcg
     3661 cacatcgaag aatagattga ccgagacggg attataaacg cgctcggcat ccggatgaaa
     3721 ccggtagcca ttgtaccaca ggtgcatctg ctccagcagt gcctcgcggc cgagacccat
     3781 ttgtgctgct gttttcgtga tttcttctga aaaattggac tccaattcga cctgcgtata
     3841 gcccagcata gtggagtagg cagggtgtat agtcaggtcc tgaaggttgt tgataccgga
     3901 aaaaatgcct actttggaaa acttgctgac gcccgtgagg aagacgaact ccagcaggtg
     3961 atcggcttct ttgagtaccg agtagaaccc gcgcaggaag tcgcggtttt cgatggcctg
     4021 aggcagttcc ttgccgaggt aatgtgtgat aggcgcgtcg tattcatcaa tgaggacaac
     4081 gactttcgcg gtagcggcca gcaccgtcgc caattcccgg aagcggtcac gggcggttgg
     4141 cgtttttaac tccacgccat attgtgccgc aatttccttc atccgttctg ccagcggtga
     4201 ctccagccca cgctgctcaa aattgatgtc tttgaaagaa atccgaatga cggggtgttt
     4261 tttaccccaa tcccatttat cctcaatcca aagccccttg aacaactcgc ggctgccgga
     4321 atacaactcg tgcagcgtgg ctaatgtgat ggacttaccg aaccgccggg gacgggaaag
     4381 aaaataggtg ccggggcttt gacacagttc gtacaaatac atcgttttgt caatgtattt
     4441 gaagccgttt tcccgaattt tgcggaaatc ctgtatgccg ataggtaatg gcggtgtggc
     4501 catgcactga tgttttcctg caaaggtaca aaacccgtcc gtaactcagt ctgccgtccg
     4561 aatggcagca ttcagattat ctatgataaa ctgtgctacg ttcatgtcgt tggtatgatc
     4621 ctgccgggtt acgcggaagt cgaggaaatt tccggcgtcg ttgaccaaca ctttacggag
     4681 gtccactgta aacggcagct tgacttcctc cccggtttct acggaaaaat tggtgttgaa
     4741 ggttttaact tgatacaatg aattggcgcc gatgtggaag gtgagcttgg cattttcgtc
     4801 ataattgccg tcgccgttca ggtcggcatt gccctcaatt ttggtaaaaa tatagcccag
     4861 tgcccaggac cagtagttgt cggtcagcgg atggccgggc ttgtaatcgc cgggctgtgt
     4921 gccgttgagg tcgggggcta tgccgatgcc catttttaca gctttgtaat tgccgggcgg
     4981 cactttggcg gcttgggtaa caacaccggc agcggcggcc gcatcgtcct gtatgttttt
     5041 aaaagaaatc agttctacct ccgaaagcgc gtgttccgtg ccgtcttctt tgatgagcgt
     5101 gacgtcggag acgtaaaaca ggaagttctg aaacttgacg ttcatgcctt cgggataagc
     5161 atagctgcgg gtgaacatgg agagcggggc gccgccgaaa tcagctttga actggaggct
     5221 taccgagccg tcgtcggttt tattgtcgcc acaggaggct gccagcaaga cggccaaaac
     5281 ggggatcaaa aagcgagctt tcatttgctt tgatttttat tgtgaaacgg ttctgactcc
     5341 tactacttca aaggacgcgg cacttctgtc ggtccatttt tgcaacaaag tttcaggtgt
     5401 ttgggttgag ggcgaaatgt cgcgcaacca acttaagtag tcaattcgga tatccaattc
     5461 cagatggtag ccctccggca cgctcacatc aaagggcagt tccaattcct gcctttgcgc
     5521 cggcaatcgc gccgctactc tgagcgtatc attgggcgcc gtttccggca aaaaacgagc
     5581 ctgctgaaaa acaaaacctt cgttcatgtt ccagtccatg cccgaagcag cgggccgcat
     5641 aggatggcca gagggcagcg attcggggtc aaccttagcg gccacatcat tcaaaccaat
     5701 gcgaaagcgc aaggctgtga acctgccgcc tttgcgaaac gcgccgacgc tgttgatgcc
     5761 gatcaatgcg gggctgacga gcagtatatc gttggtgatg ctgtcttttt gcaggcttcc
     5821 gccggatgta aacacttgaa cttcaatgcg attgctcagc ggtgtttccg agccgtcggc
     5881 ctttaccaat cgcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcagcgagg ggtacacgca gcagtcgtcg
     6061 ggacaagcta cgtccgcatc gggtgcaaaa ttggcggcgg aaatatccag acaaccttct
     6121 ttcggctcat aacaaccggc gagcaggaac agtgcaaaaa tggggagaat gtatttcaaa
     6181 gtgtatgtgt gtttgagtga cgaaatgatt tccggccttg cgtcgcaaac tgcaaggggg
     6241 tgatttcata agctcttgcc ttgctcaagt acccggatgg aaaccgattt ttttagccgg
     6301 ctggtcgtcg tcgtagtctt cccactgggt gatttccgac atgaattctt ccagatgtcg
     6361 cagaaaaaaa tcgggttgca gctcttcggg gcgatagatg gcaatggttt tcggctcgcg
     6421 cggctggccg tagctttcga tgaaggggta attgatgagc acgatgattt tgccgttgaa
     6481 caattgctgg tagatgagcg atccgcgttg tttaataaaa tctcttttga tatggctgtc
     6541 aatgcgttgc gccaggccgc ttttttcaac gcccaaagtg agtactacgg cttccaaatt
     6601 gagtacaggc gcttgcacct cgatgaaagc gtccaattcc accgctttgg agatatcctt
     6661 gagcgttttt acgatcctgt cgcggaggct gcttttccat tcttcccgga acttctcggt
     6721 gttcgcaatg atggttttat actcctgcac cttgcgttgt aaggcaaaat aatttgcatc
     6781 catgtttgtt ttgtttttta aaatgcaaag ctacgttttt tggcctcaat ctgccccgac
     6841 ggacaggttt tagctaaggg cgcgaatccg ccccggcgga caggtttacg cgaatgcgag
     6901 ggggccagct tagtgaaggg gtgactacgg aactgaaaca tactgagtca ccccttcact
     6961 acccccgaaa ccccaaacct cgaacccgaa atcagaaacc cgaaatcagg aatccgaaat
     7021 ccgaaatcag cccgttttcc ctacctttgc ctcctcaaaa cacacttggc gcgcatgaac
     7081 cacgaagccc tggcacaatt gttttaccgc gaactgttga aagcagtttc cgtacccgac
     7141 ttggacgacg aggcgcgttg cgaacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctttt cgcaaggtcc
     7321 tcgccgatcc cgcccgacgc cgcaccgcgc ttcccgctta cctgctgccc ctgggcatca
     7381 aagccctcgg ccaggccatt accgccttgc tgggactcaa tccgcctgcc gagcttcaat
     7441 cgtacatccc acgcgagtgg ccgctgccct accggcctgc cgaacgcgcc gcatttcacc
     7501 acaaagtgcg ggtcgttgca ctttccgacg atgccgagca atgtaccttg ctggcacgca
     7561 ctgaagaccg cccggaagaa accgcccgca tagcctacaa tctggccgat cacaacgaga
     7621 atttcaatcc taccattgac accctgcgaa gtgtttttcg tttccccgtc accttgcatc
     7681 tcattgatgc tgaggtagat caagagggtg tttaccggcc cagagccttt gtggtcgaac
     7741 ctgatttcct gctcgatgtg tccgccgttg caggttgttt ccgggagcag gcagccgaac
     7801 cctggctttt tctgctcgac aaattcaagc ctttttcggt atccaaacac cgcatcatcg
     7861 gcaacattgc caactttttt ttggacgagt tgatgaccga ccccgaagcg gaattcaaaa
     7921 ctgtttttgc caaagttttc aggctcagcc ccctggcgtt ttgtttgctg cccgatgccg
     7981 aagtcaaaga aattcaccaa agcgtgcaga aacatttcgt gacgctgcgc accatggtac
     8041 tccgcgattt tgccgccaga gacatcgcgc ccgaacactg ctttctggag ccgtcctttt
     8101 attcggaaac gtatggtttg cagggccgct tggatgtgtt ttgctaccaa cccgatgatg
     8161 aatcaaaggc cgccatcgtg gaactgaaaa gcggcaaacc cttcaatacc aacggctacc
     8221 gcatcaatca cgaccatttt gtacaaaccc tgctctacga tctgatgatt cagtcggcct
     8281 tcgaccgccg cctcaacacc gccaactaca ttctgtacag cagccaggat gaagacccgt
     8341 tgcgttttgc gccccgcatt cgctcgcagc agtacgactc cctgcaggtg cgcaatcaga
     8401 tggccgctat cgaacgcctc ctgcaacgac ttggtaatca gcaggatgtt gatcttgccg
     8461 agcagggccg ccgcttgttc ggccgcatca gtccggggcg attgccggcg ctcaaggggt
     8521 ttgcccgaaa ggatttggaa gctttcgagg ccatgtattt cggcatgacc gatctggaac
     8581 ggcggtactt cattgccttt gccggcttca ttgcccgcga gcatcaattg gccaaaaccg
     8641 gcctcatcgg aaccgacacc gtgaacgggc tggcggccct gtggctcaat accacgcagg
     8701 aaaaacgcga tgggtttgaa agcctcagcg gactgaaaat agcccgcatg atggcccgcg
     8761 acgaggagcc gattctggag ttggaacgcc cggcaaccgg agacgtgctg gccaatttcc
     8821 gcctcggaga cattgcggtg ctgtacccgg ctcaggagaa aggcggcgtg ctctcgcacc
     8881 agattttcaa atgcaccatc atcgaaatca gttcgcaaaa agtggccgtg cgcctgcgct
     8941 ccaaacaatt caacgacagc ctctttcgcc gttttgagtt ttggaacatc gaacacgacc
     9001 tgctcgatac ctccttcgtg gccatgtacc gcgccctgta cgagtttgcc ggcgcgcccg
     9061 cccatcgaaa aaaactcctc ctcggcatcg cagcgccccg gcaggcacag cccgaagaca
     9121 tcccgctctc tgccgaactc acctccgaac agcaacacat cctccgccag gccattgccg
     9181 cgcccgatta ttttctactc tgggggccgc cgggcaccgg caaaaccagc atgatgctca
     9241 agcacttagt gagttatctc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccttctg
     9301 ctggcctaca ccaaccgcgc cgtggacgag atgtgcgagg ccgtcgaatc catcggcccc
     9361 gatgtgcggc ggcactattt ccgcatcggt tcgcgctatg ccacgcaccc cgatttccac
     9421 ccgcaactgc tcgacgcgct catcgaaaac gtcggcacgc gccgcgatct caaagcgctc
     9481 attgacagcc gccgcattgt gctcgccacg gtggcttcca tcggcggcaa gcctgaaata
     9541 ctgcaactca aatccttcgc ccgcgtcatc atagacgaag cctcccagat tctggaaccc
     9601 atgctggccg gcctcctgcc gcgattcgag cgcttcatcc tcatcggcgn nnnnnnnnnn
     9661 nagcaattgc ccgccgtcgt gggccaatcg cccgatgcct cccgcgtaga agactccgcc
     9721 ctgcaatcca tcggcctgca caacctgcgc aactcgcttt tcgagcgcct gttccgcctt
     9781 tgtatccaaa ataactacac ctgggctttc gctcaactca gccggcaggg ccggatgcac
     9841 cgcgacatca tggcctttcc caactcgttt ttttaccaaa acaccctcca aatcctcccc
     9901 gacgacacgc ccggcgccct ccgccaaacg gcaaacctcc ccttcgatgc ccaggccgcg
     9961 cgctacggtc tcgaaagcgt ggtggcgctc cgccgcgtat tgttcctctc cacgcccacc
    10021 gacgaaggag gcgtcgcaca aaaaaccaat cgccacgagg ccgtgcttat tgccgacctc
    10081 gtcagcgcat tccaacgcct gtacgatggc cgcctgctct ccatcggcgt catcacgccc
    10141 taccgcgcac agattgcccg catccgcgcc gaactcgaaa gccgccagat tcagcccgac
    10201 gaactcacca tagataccgt agagcgctac cagggcggcg cccgcgacgt gatcctcatc
    10261 tccttgtgta ccaatagttt gagtgcgctt tcatcgctca tttccctctc cgacgacggc
    10321 gtggaccgca aactcaacgt ggccctcacc cgcgcccgcg agcatgtggt ggtggtgggc
    10381 aatgccgaat tgctccgcga gaatgagacg tatgcgcaat ttatagccat gtacagtgtg
    10441 tgaggggtgg taggggtgag gggtgagtat gtgagtagtg agggctgctt gggcgtgccc
    10501 ccgctcctgt tgaatcttat gcggggtcgg gtgcctcggt cgtcctccta acgtcgttcc
    10561 tcccttcggc cccctcacgc
//
LOCUS       DAORLF010000119        10532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3374_length_10532_cov_1.961726, whole genome shotgun sequence.
ACCESSION   DAORLF010000119 DAORLF010000000
VERSION     DAORLF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_10532_cov_1.961726"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    219..428
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <429..1040
                     /locus_tag="PK198_11065"
     CDS             <429..1040
                     /locus_tag="PK198_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRF39323.1"
                     /translation="ALIVAFEPLLVVLLLWIMRGKKPAGRSIAGVGIGVAGMALLVLQ
                     DTLIADRNTALGVLVIFIGILSWGYAAIWVGRAKLPASSMQTAAIQMLGGGAALMLFS
                     LVVGEWTDFQWSQVSAKSWLAWLYLVGFGSILAFSAFNYLLARVSPEKVVTTNYVNPV
                     VALLLGWGLNGEVISLQSLAASILLLAGVFFIVSRKKDEAPAH"
     assembly_gap    1079..1119
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1120..1519)
                     /locus_tag="PK198_11070"
     CDS             complement(<1120..1519)
                     /locus_tag="PK198_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39324.1"
                     /translation="MSFRARFDLGKTNPVNLVIGILIMVAIFYGLFKLTQWLFTLLYY
                     VAPLLLIATLIIDINVVKEYIQTLGKLVKRNTPLGIAAIALSVVFAPVPIAYMFIKAI
                     MKRKVDKYIENQRRIEEGDLVDFEELERRPL"
     gene            1652..>1931
                     /locus_tag="PK198_11075"
     CDS             1652..>1931
                     /locus_tag="PK198_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRF39325.1"
                     /translation="MKAFILADKEKVEFREDMPKPVAQADEVLVQIRAAALNHRDVWI
                     SKGLYPGIVFPSILGSDGAGICEGREVVINPSIGWGANPAIQAKDYQIL"
     assembly_gap    1932..1970
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1971..2622
                     /locus_tag="PK198_11080"
     CDS             <1971..2622
                     /locus_tag="PK198_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRF39326.1"
                     /translation="AIPAAQIYDKPAHLSMEEATAIPLAGLTAYRAVFSKGQLTAGQK
                     VLVTGIGGGVALWAMQLALAGGAEVYVTSGHDAKLHKAKVLGAHGGVNYRESDWAKTL
                     RQQAGSFDLVIDGAGGEGLAQVLGCCAPGARVVVYGGGRGAVPQLSPQLLFWKQISIL
                     GTSMGTDAEFAALLEMVSRCQIKPVVDSVFALEDTAAAFARMAAGEQFGKIVIRTS"
     assembly_gap    2724..2736
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2737..>3070
                     /locus_tag="PK198_11085"
     CDS             <2737..>3070
                     /locus_tag="PK198_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRF39327.1"
                     /translation="GNVQVADELEAFRPFRAVYGNIDGAPLRARYPEDLRFTCEGVEV
                     FMTHIGGYPGKYHPRIRPLLKAATPRLFICGHSHILKVIPDKELGLLHINPGACGIEG
                     FHKVKTIVR"
     assembly_gap    3071..3141
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3142..3908)
                     /gene="trpA"
                     /locus_tag="PK198_11090"
     CDS             complement(<3142..3908)
                     /gene="trpA"
                     /locus_tag="PK198_11090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRF39328.1"
                     /translation="MNTNRITALFASGKKNLLNIYFTAGYPALYDTADIAQALAAAGA
                     DIIELGMPYSDPLADGTTIQQSSEQAIANGMTMELLFEQVEEIRRRTQVPLVWMGYLN
                     PVMQYGEDRFLKRCAETGIDGLIIPDLPLHEYEASFKSKAEALGLVFSFLITPQTPES
                     RIQDIARLSSGFVYMVSSASITGAKAGISESQVAYFERIAAMNLPQPRLIGFGISDKD
                     SFQTACRYAQGAIIGSAFIKALGEGRGAAEVARGFVEG"
     gene            complement(3956..>5765)
                     /locus_tag="PK198_11095"
     CDS             complement(3956..>5765)
                     /locus_tag="PK198_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRF39329.1"
                     /translation="VDQAKAELESVKGQYELLAGQYRKLMKRQSDITARIHQLSGGDM
                     HELSGHIAAHFERIMEYEERIAGLAKHTDASDYALSPGAALQQRLRDADQIIEQKKRN
                     RDQLLELGKLMDAAEAEKAAFAPRLEAKARELILAQEQLRAAEERLGERRDQFIALGE
                     ELRGLLRLYGIHFQEYPSKEDIEQLRRQQTRYSDTQKQLAEGITRKALLEQEIRQNNQ
                     LAQASAQRLMELQAAADRQGAALTQLKEQRGLIPVGQDPRRLRDEERERLQQAETAAR
                     EARERLSPAEAECAAAAATLAGRSSDVQLATQSAADLRDKLEAAARAAGFDHAAALRA
                     ALLPPEEALLIEQRRAELERRAAELQIAARDTEQTLTQEFDRFPDAPSEAELDVALAA
                     LRAQIETQQQQWGALQVILDQDAQRRAEAADLQHRLEAQAAEHRRWARLDELIGAADG
                     KKFRVFAQGLTLRKLVGLANEHLQRLSGRYILHRRSDDNLELEMVDTFQADNRRSVNT
                     LSGGESFLVSLALALGLSDLAGRNAAIRSLFIDEGFGTLDENSLDLAVSTLENLQAAG
                     KTIGVISHVKEMKERIGAQIQVRKSGNGFSTVHISG"
     assembly_gap    5766..5920
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5921..7619)
                     /locus_tag="PK198_11100"
     CDS             complement(<5921..7619)
                     /locus_tag="PK198_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39330.1"
                     /translation="MKIRKISIQNLNSLRIKTSIDLCAPPLSNVGLFAITGDTGAGKT
                     TILDAITLALYGRVHRNKDVKEVMSFGAVESLAEVEFEAGDGIYRSKWSIWRAHRKED
                     GNLLGPVRELSRYNPETGEFDILAQKKGDSDQRIEQITGLDYDRFCRSVLLSQGDFAA
                     FLRAGEQERSELLERITGTEVYSELSKAAFQRKKAEEERLRQLRLELDSLRVLPDEER
                     DELLARLEETRRLSADLKKNADQTNEQLQWRRQLKSLQQKLDAARQSLSQLDAARQAA
                     LPQLERLELHRRAAAQQSHIERADEAEARLAVLQGEEARFQQVLETKQALLEQIRALA
                     ATTDSDWNVQKKSFLEREPLFEQAIETDAQIARQTEALQKAGQEVHSENERLFNFQQS
                     LQKAEQELDGVRRALAEYEQWLADNAVAESLAEQLPLIEDKIEELRNAFKERSRSEKN
                     VDEARAARDGFLTENAHLETEEKTLNERLEQLRADFKALLPENTPTTRAELLQSASVE
                     IEHLQNSKSHLEQLSQLYEAYQKLLGELNAYEDQLNGLQYEELDINKEMMTLLDALDA
                     "
     gene            complement(7616..8839)
                     /locus_tag="PK198_11105"
     CDS             complement(7616..8839)
                     /locus_tag="PK198_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRF39331.1"
                     /translation="MKFLHTSDWHLGQKFLSNDREDEHRRALLRMVELVESEQADALI
                     IAGDVFDIGNPPHYARRLYYDFLTRMLKSSCCRHIVITGGNHDSPSMLNAPRDLLRAL
                     RVHVVGAATGNIEDEIVELRDDQDRLEAVVAAVPFLRDGDIRASVSGETAADRVEQIK
                     TAILQHYQQVGEYCERYRGQNIPIIATGHLYAAGASASEKQDNIYIGNTDNIDARHFP
                     GIFAYVALGHLHRLQSVGGLPHVWYSGSPIPLSFSETKDDKAALLVSFEGDKLLEVKP
                     LPLPVFRRLKTIEGSLEKVEADLLKFGSKPDRDLSSWVDIIVHAEQFIPQLDTRLHEL
                     AQGMDLEILKIRLHRPAAAADALSAAFDLDSLAPEEVFEQRCRAFAMNDEELNELKTT
                     FAELRSWMQQQEVLP"
     assembly_gap    8911..8920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8928..>9256
                     /locus_tag="PK198_11110"
     CDS             8928..>9256
                     /locus_tag="PK198_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39332.1"
                     /translation="MIARLEQAGKQYKVGDQTITALQPTSMDIHEGELLLIIGPSGSG
                     KTTLLSLLGCVIYPSQGRLWVDGMEINRLKDSQLAKLRLNTIGFVFQNFNLLAPLSAE
                     DNVMMPLR"
     assembly_gap    9257..9353
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9354..9647
                     /locus_tag="PK198_11115"
     CDS             <9354..9647
                     /locus_tag="PK198_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39333.1"
                     /translation="ADALSGGQQQRIAIARALVTNPKLLLCDEPTASLDKDSLSVVMD
                     ELRSLADSGKSVCVVTHDPRLKPYAHRIVEVNNGVVSAVREGAEGSDDQRRSD"
     gene            9724..>10532
                     /locus_tag="PK198_11120"
     CDS             9724..>10532
                     /locus_tag="PK198_11120"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF39334.1"
                     /translation="MRYLLPMLAIALFLQSCGPAEDQKAAAAEEAAVRPDTPLVVREI
                     VGIARIEPPEKIISINAQTAGYVREIRFGEGSRVQKGDVLLLLDNEVEQAQLRQAQSK
                     LQTQRAAITAAEASLESLRVKRNNARNTLERNQKLAAGNALTAQQLDDSRFAADELDK
                     QVSAQQAAVAQQQSRLKELEADLAYYQTLLQQRTLTAPMTGTFLSSGIKPGQYITNSS
                     VLGELAADGPYQAVTEVDELFAGRVQLGQRALVRLQGSTEPLATGKVVYVAP"
BASE COUNT         2037 a         2676 c         2898 g         2285 t
ORIGIN      
        1 atcggtcatc gggcggcaat cgcggcgcaa agatagcaat ttagattaaa aaaacgggat
       61 tgcttcctgt aaatccatca caatctattt tgataccttt gtgccctcaa atcaacaaaa
      121 tgcaatccaa cacaggctcc tcgcggctca tcattgctgc gtttgcagtg gtttatatcg
      181 tgtgggggtc cacttatttg gtcaattact ttgccattnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnngc gctgatagtg gctttcgagc cgctgttggt agtgctgttg ctctggatca
      481 tgcggggcaa aaaaccggcg ggccgcagca tagccggggt gggcatcgga gtggcgggca
      541 tggccttgct ggtgctgcaa gacacactca tcgccgacag gaatacggca ttgggcgtgc
      601 tggtcatttt tatcggcatc ctgtcctggg gctatgcggc catctgggtg ggacgcgcca
      661 aactgcctgc atcctccatg caaacggcgg ccatacaaat gcttggcggt ggcgccgcac
      721 tgatgttgtt cagcttagtc gtaggcgaat ggactgattt tcagtggagt caggtgagcg
      781 cgaaatcatg gctggcctgg ctgtacttgg tgggattcgg ctctattctg gccttttctg
      841 ccttcaatta tctcctggcc cgggtgtcgc cggaaaaagt ggtaactacc aattatgtca
      901 atccggtagt ggccctgcta ctgggctggg gcctgaacgg cgaagtcatt tcgctgcaat
      961 cattggcggc ttcgatactg ctgttggccg gggtgttttt tattgtttcc agaaaaaaag
     1021 acgaagcgcc ggctcattaa aacatctcta aaccggcacc ctcgaagctc attttaaann
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc caatgggcgt ctctccaatt
     1141 cttcaaaatc caccaaatcg ccttcctcta tacggcgctg gttttcaata tacttatcta
     1201 ctttgcgctt catgatcgct ttgatgaaca tgtaggcaat cggcactggc gcaaaaacca
     1261 ccgacagcgc aatggcggcg ataccgagcg gcgtgttgcg ctttaccaat ttgcctaaag
     1321 tctgtatgta ctctttcacc acgttgatat ctataatcag cgtagcaatc agcagcagcg
     1381 gggctacata gtatagcagc gtgaagagcc attgtgtcag tttgaacagc ccgtaaaaaa
     1441 tggcgaccat gataaggatg ccgatgacca aattgacggg attggttttg cccaaatcga
     1501 atcttgcccg aaaactcata attgcattga atttattaca ggcgaatata cagcctttct
     1561 tccaaaaaag ttggattcct ttcgaccaat ggtgtgcgcc tgctgccgag cgaaaggaaa
     1621 aaacataact ttgccgaaca agcatccttt tatgaaagca tttattctgg ccgataaaga
     1681 aaaagtcgag tttcgggaag acatgccgaa acctgttgct caggccgacg aggttttggt
     1741 gcaaatacgc gccgccgccc tcaatcatcg cgacgtatgg atttcaaagg gcctgtatcc
     1801 gggcattgtt tttccctcca ttttgggttc cgacggtgcc ggcatctgcg aaggccgcga
     1861 ggtcgtcatc aatccgagca tcggctgggg cgccaatcct gccatacaag ccaaagatta
     1921 tcaaatactt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgccattccg
     1981 gcagctcaga tttacgacaa accggcacac ttgagcatgg aggaagccac agccattccg
     2041 ctcgccggct tgacggccta ccgggctgtg ttcagcaagg gccaactcac agcaggccaa
     2101 aaagtgctcg tcaccggcat cggcggcggg gtggcgctgt gggccatgca gttggcgttg
     2161 gccggcggcg cagaggtata tgtcacttcg ggccacgatg caaaactgca caaagccaaa
     2221 gtattaggtg ctcatggcgg cgtcaattac cgggaatccg actgggccaa aacgctgcga
     2281 caacaggcag gcagcttcga tctcgtcatt gatggcgcgg gcggagaagg gcttgcacag
     2341 gtcttgggct gctgtgcgcc gggcgcccgg gtggtggtgt atggcggagg tcgcggcgct
     2401 gtgccgcagc tcagtccaca gttgttgttt tggaaacaaa tctccatact cggcacctcc
     2461 atgggaaccg atgccgagtt tgcggccctg ttggaaatgg tttcccgttg ccaaatcaaa
     2521 cctgtagtgg acagcgtgtt cgcattggaa gacacggctg cggctttcgc ccgaatggct
     2581 gccggcgagc agttcggcaa aatcgtcatc cgcacctcat gaagcgcatc ggcctccttt
     2641 cagatacaca ttcctggctc gatccgcttg tgtttcagta ttttgaatcc tgcgatgaaa
     2701 tctggcacgc cggcgacatc ggcnnnnnnn nnnnnnggca atgtacaagt ggccgacgaa
     2761 ttagaagcgt tcaggccatt cagagctgta tatggcaata ttgacggagc gcccctgcgc
     2821 gctcgctacc cggaagatct gcgttttacc tgtgaagggg tggaggtttt tatgacccac
     2881 atcggcggct accccggcaa gtaccacccg cgcatccggc ccttgctgaa ggccgctacg
     2941 ccgaggttgt tcatttgcgg acactctcat atccttaaag tcatacccga caaagagttg
     3001 gggctgctcc acatcaaccc cggcgcctgt ggcatagagg gctttcataa agtaaaaacc
     3061 attgtccgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nccctccaca aaccccctcg ccacctctgc cgctccccta
     3181 ccctcgccca atgccttgat gaacgcgctg ccgatgatgg cgccttgtgc atagcggcag
     3241 gccgtctgaa agctgtcctt gtcggaaatg ccgaagccga tgagtctggg ctgtggcagg
     3301 ttcatcgccg caatgcgttc aaaataagca acttgagact cgctgatgcc ggctttggca
     3361 cccgtaatgg acgcgctgga aaccatgtaa acgaagcccg aagacagccg cgcaatatcc
     3421 tgaatgcgag attcgggcgt ctgcggggta atcaaaaaac tgaacaccaa acccagcgct
     3481 tccgccttgc ttttgaaact cgcctcgtac tcatgcagcg gcaaatcggg gatgatgagg
     3541 ccgtctatac cagtttcggc gcagcgcttc aaaaagcggt cttcgccgta ctgcataacc
     3601 ggattgaggt agcccatcca caccaaagga acctgcgtgc ggcggcggat ttcctccact
     3661 tgttcaaaca gcaattccat ggtcatgccg ttggcgatgg cctgctcgct gctttgttgg
     3721 atggtggttc cgtcggccaa aggatcggag tatggcatcc ccagttcgat gatgtcggcg
     3781 ccggccgcgg ccaatgcctg agcgatatcg gcggtgtcgt acaatgcagg gtaccccgcc
     3841 gtgaaataga tattgagcag attttttttg ccggatgcga agagcgctgt gatgcgattg
     3901 gtgttcataa aattgattta caagacatta ggcaataaca aagtaaaaaa gaacatcagc
     3961 ccgaaatatg caccgtgctg aatccattgc cgcttttcct tacctgaatc tgagcgccga
     4021 tgcgttcttt catttctttg acgtgggaga taaccccaat ggttttgccg gcagcctgta
     4081 ggttttcgag ggtggaaacg gccagatcga ggctattttc atccagagtg ccgaagccct
     4141 cgtcaatgaa taaggagcgg atggcggcgt tgcggcccgc cagatcggac aggcccagcg
     4201 ccagtgccag actgaccaaa aagctctcgc cgccggaaag ggtattgacc gagcggcggt
     4261 tgtcggcctg aaaggtgtcc accatttcga gttcgagatt gtcgtcgctc ctgcggtgca
     4321 ggatgtacct gccgctgagg cgttggaggt gttcattggc cagcccgacc aatttgcgca
     4381 gggtaagccc ttgcgcgaaa acgcggaatt ttttgccgtc ggcagcgccg atgagttcgt
     4441 ccaaacgcgc ccaacggcgg tgctcggcgg cttgcgcttc gaggcgatgc tgtaaatcgg
     4501 cggcttcggc ccggcgctgc gcatcctgat cgaggatgac ttgcagagcg ccccattgct
     4561 gttgctgcgt ctcgatctgc gcccgcaggg cggccagcgc cacgtcgagt tctgcctcgg
     4621 agggcgcatc ggggaagcgg tcgaattcct gtgtcagcgt ttgctccgta tcgcgggcgg
     4681 cgatctgcaa ctctgcggcg cggcgttcca attcggccct gcgctgttcg atgaggaggg
     4741 cttcttcggg cggcaacaga gcggcgcgaa gggcggcggc gtggtcaaaa ccggcagcac
     4801 gggcggcggc ttcgagtttg tcgcggagat cggcggcgga ttgcgtggcc aattgcacat
     4861 cggagcttcg gccggcaagc gtggcggcgg cggcggcaca ttccgcttcg gcgggagaga
     4921 gccgttccct cgcctcacgg gcagcagttt cggcctgttg aaggcgctcg cgctcctcat
     4981 cgcggaggcg gcgcgggtct tgtcccacag ggataaggcc gcgctgttcc ttcagttggg
     5041 tcaatgctgc accctggcgg tcggcggcgg cctgtagttc catcaggcgc tgtgctgatg
     5101 cctgtgcgag ttggttgttt tgccgtattt cctgctccag cagggctttc cgggtaatcc
     5161 cttcggccag ttgcttttgg gtatcggagt agcgcgtttg ttgccggcgc agttgttcga
     5221 tgtcttcttt ggaaggatat tcctgaaaat ggataccata caaacgcaac agcccgcgca
     5281 attcttcgcc aagagcaatg aactgatcgc ggcgttcgcc gaggcgttct tctgcggcgc
     5341 ggagttgttc ctgtgccaaa atgagttcgc gggctttggc ctccaagcgc ggcgcaaagg
     5401 ctgctttttc ggcctcggcg gcatccatga gcttgcctaa ttccaacaac tgatcgcggt
     5461 tgcgcttttt ctgctcaatg atttgatcgg catcccggag gcgctgttgc agtgccgcac
     5521 cgggcgacag ggcatagtcg gaggcatcgg tgtgcttggc taagccggca atgcgttcct
     5581 cgtattccat gatccgttca aaatgcgcgg caatgtgccc gctgagttcg tgcatgtctc
     5641 cgccggaaag ctggtgaatc cgggcggtga tatccgattg gcgcttcatc agtttccggt
     5701 actgcccggc cagcaattcg tactgccctt tgacgctttc caactctgct ttggcctgat
     5761 ccacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcgtccaa agcatccagc
     5941 agggtcatca tttccttgtt gatgtccaac tcttcgtatt gcaagccatt gagttgatct
     6001 tcgtaagcat tcagttctcc caacagtttt tggtatgctt cgtagagttg cgaaagttgc
     6061 tccaaatggc tcttgctgtt ttgcaggtgt tcgatttcaa cggaggccga ctgcaacaac
     6121 tctgcgcggg tagtgggcgt attttcgggc aagagggctt taaaatcggc gcgcagttgc
     6181 tccaaacgtt cattgagcgt cttttcttct gtttccaaat gcgcattttc ggtcaggaat
     6241 ccgtcgcggg cggccctggc ctcgtccaca tttttttcag aacggctgcg ctctttgaag
     6301 gcattgcgca gttcttcaat tttgtcttcg atgaggggca gttgttcggc cagtgattct
     6361 gccacggcat tgtcggccag ccattgctcg tattcagcca atgccctccg cacgccgtcg
     6421 agttcctgtt cggccttctg gagcgattgc tgaaagttaa acaggcgttc attttcagaa
     6481 tgaacctcct gcccggcctt ttggagcgct tcggtttggc gggctatttg cgcatccgtt
     6541 tcgatggctt gttcaaagag cggctcgcgt tcgaggaaac tctttttttg gacgttccaa
     6601 tccgaatcgg ttgttgcagc cagcgcccgt atttgctcca gcagcgcttg ctttgtttcc
     6661 aaaacttgct gaaaacgagc ctcttctcct tgcaaaacgg ccaaccgggc ttcggcttcg
     6721 tcggcgcgct ctatgtgcga ttgctgtgct gcggcacggc ggtgcagttc taagcgttcc
     6781 aattgaggca gggcggcctg ccgggcggcg tccaactgag agagagactg ccgggcagca
     6841 tctaattttt gttgcaagga tttcaattgg cggcgccatt ggagttgctc gttggtctga
     6901 tcggcatttt ttttgagatc ggccgaaagg cggcgggttt cctccaagcg ggccagcagt
     6961 tcgtcgcgct cttcatcggg caacacgcgc aggctgtcca attcgagcct aagctggcgc
     7021 aggcgttctt cctctgcttt ttttcgttgg aaggccgctt tggagagttc ggaatacact
     7081 tctgtgcccg tgatgcgctc taataattcg ctgcgttcct gttcgccggc gcgcagaaag
     7141 gcggcaaaat cgccctgaga gagcaacacg gagcgacaaa aacggtcgta atccaagccg
     7201 gtgatctgct cgatgcgttg gtcggaatcg cctttttttt gagccagaat gtcgaactcg
     7261 ccggtttcgg gattgtaacg agatagttct cgcaccggcc ccagcaggtt gccgtcttct
     7321 ttgcgatgcg cccgccagat actccatttg gagcgataaa tgccgtcgcc ggcctcaaat
     7381 tccacctcgg cgaggctctc cactgcgcca aacgacatga cttctttgac gtctttgttg
     7441 cgatgcaccc ggccgtagag cgccagggtg atggcgtcga ggatggtggt tttgcccgcg
     7501 cccgtgtcgc cggtgatggc aaacaggccc acattggaca aaggcggcgc gcagaggtct
     7561 atgctggttt tgatgcgcag cgagttaagg ttttgtatgc tgatcttcct gattttcatg
     7621 gcaatacctc ctgttgttgc atccagcttc tcagttcggc gaaggtggtt ttcagttcgt
     7681 tcagttcttc gtcgttcatg gcaaaagccc ggcagcgttg ctcaaacacc tcctccggcg
     7741 cgagggaatc gaggtcaaac gcggcggaga gtgcatcggc ggcggcagca ggccgatgga
     7801 gcctgatttt gagaatttcc aaatccatgc cctgcgccag ttcgtgcaga cgggtgtcca
     7861 actgcggaat gaactgctcg gcgtgtacga tgatgtccac ccaggagctg aggtcgcggt
     7921 cgggtttgct gccgaatttc agcaggtcgg cctccacttt ttccaagctc ccctcaatgg
     7981 ttttaagtct tctgaaaacc ggcagcggca aaggcttcac ttccagcaac ttatcgccct
     8041 cgaacgaaac caacagggca gccttatcgt ctttggtttc gctgaaactg agcggaatgg
     8101 gcgagccgga gtaccacaca tgcggcagcc cgcccaccga ttgcaggcga tgcaggtggc
     8161 cgagggctac atacgcgaag atgccgggaa agtgccgggc gtctatgttg tcggtattgc
     8221 cgatgtagat attgtcttgt ttttcggagg cggaagcgcc ggcagcgtag aggtggccgg
     8281 tggcgatgat gggtatattt tgcccgcgat agcgctcgca atactctcca acctgttgat
     8341 aatgctggag aatggcggtt ttgatttgct ccacgcgatc tgcggcggtt tcgccggaaa
     8401 cggaggcgcg gatatcgccg tcgcggagga aaggcacagc agctactacg gcttcgaggc
     8461 ggtcctggtc gtcgcgcagt tctacgattt catcctctat gtttccggta gcggcgccga
     8521 caacgtgtac ccggagggcg cgcagcaggt cgcggggcgc attgagcatg gagggcgagt
     8581 cgtggttgcc gccggtgatg acgatgtggc ggcagcagga actcttgagc atccgggtga
     8641 ggaaatcgta gtacaaacgg cgggcataat gcggcggatt gccgatatcg aacacatctc
     8701 cggcgatgat gagggcatcg gcctgctcag attccactaa ttctaccatg cgcagcagag
     8761 cgcgtcggtg ctcatcttcg cgatcgttgg agaggaactt ttggcccagg tgccagtcgg
     8821 aggtgtggag gaacttcatg cagtttttag tagaaagtgc cgggcaaagg tagcaatatc
     8881 aaaaccaaag ccaaacaact ctgtttcact nnnnnnnnnn caaatgaatg atcgcacgtt
     8941 tggagcaggc gggcaaacag tataaggtag gcgaccaaac catcacggcg cttcaaccta
     9001 cctcgatgga catccatgag ggcgagttgc tgctcatcat aggcccgtcg ggatcgggaa
     9061 aaaccacgct gctgtctttg ctgggctgcg tcatctatcc gtcgcagggg cggctgtggg
     9121 tggacggtat ggagatcaat cggctcaaag acagccaact ggccaaactt cgcctcaaca
     9181 ccattggttt tgttttccaa aatttcaacc tgctggcgcc gctcagcgcg gaagacaatg
     9241 tgatgatgcc tttgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgacg
     9361 cgctctcggg cggacaacaa cagcgcatcg ccatagccag agctttggtg accaacccca
     9421 aactgttgct ctgcgacgag cctaccgctt cattggacaa agactctctg agcgtggtca
     9481 tggacgaact gcgaagtctg gccgattccg gcaaatcggt ctgtgtcgtc acccacgacc
     9541 cgcgcctcaa accttatgcg catcgcattg tggaggtgaa taacggggtg gtgagcgctg
     9601 tgcgtgaggg ggccgaaggt agtgacgacc aaaggaggag cgactgatgc acccgacggt
     9661 gcattggatg ctaccggagc accggcacgc ccaaaaaatc tataaaaacc gtaataacct
     9721 gacatgagat acctccttcc aatgctggcc atagcgctgt ttctgcaaag ttgcgggccg
     9781 gctgaagatc aaaaggccgc tgccgctgaa gaagccgccg tgcgcccgga tacgcccttg
     9841 gtagtgcgcg aaattgtagg catagcgcgc atagagccgc ctgaaaaaat tatttccatc
     9901 aatgctcaaa cggccggata tgtgcgcgaa atcaggtttg gggaaggctc gcgggtgcaa
     9961 aaaggcgatg tgctcctcct gttggacaat gaggtggagc aggcgcaact gcggcaagcg
    10021 cagagcaaac tacagaccca acgcgccgcc ataactgctg ccgaggcatc gctggaatcg
    10081 ctgcgcgtga agcggaataa cgcccgcaat acgctggagc gcaaccagaa attggccgcc
    10141 ggcaatgccc tgacggccca gcaattggac gacagccgct ttgctgccga tgagttggac
    10201 aagcaagtga gcgcccaaca ggccgccgtg gcacagcagc aaagccgcct caaagaattg
    10261 gaagccgacc tggcctacta ccaaacgctg ctgcaacaac gcacgctgac ggcccccatg
    10321 accggcacgt ttttatcgtc gggcatcaag ccggggcagt acatcaccaa cagcagcgtg
    10381 ctgggggaat tagccgccga cgggccgtat caggctgtga cagaggtgga cgagttgttt
    10441 gccgggcggg tgcagttggg gcagcgggct ttggtgcgtt tgcaaggctc gacggagcca
    10501 ttggctacgg gcaaggtggt gtatgtggcg cc
//
LOCUS       DAORLF010000120        10489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3389_length_10489_cov_1.983132, whole genome shotgun sequence.
ACCESSION   DAORLF010000120 DAORLF010000000
VERSION     DAORLF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_10489_cov_1.983132"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>890
                     /locus_tag="PK198_11125"
     CDS             <1..>890
                     /locus_tag="PK198_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39335.1"
                     /translation="NEAYVLQNLGAPPRVQPGKGWATTFSQLRSQLSTEWRGIYRSVA
                     FVLLALLGLLNCVPNMFYANESYGTHALPVTYTMVDIIRGSFYLFTIIIMVYFSGAVV
                     WKERIARMNDITDALPVKNWTMMLGKYLSVLGVLFVLQVLVIAAAVMAQSAMGYHRYE
                     LWVYIRELLVIDMLGFAVTLALSFLVHALSPNMYLGFFIVVILLIVNSFAWGALKIQS
                     NMVQFGATPSYTLSDFYGYQPFFKELFWFNSYWLLFGVLLLVGAVLLWPRGRFSAWRE
                     RWFNAGLEWKQYRVFGVSALA"
     assembly_gap    891..900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <901..2747
                     /locus_tag="PK198_11130"
     CDS             <901..2747
                     /locus_tag="PK198_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF39336.1"
                     /translation="LTAGWTFYNTQIVNRYESVKKEERAQYDYEIKYKKYSQRRQPRV
                     YAARYDIDLRPETRSLRLEGVFKTRNIYTQSIDTLWVNTPYNGDFELRNERLRPLLDD
                     SLLHWQMYKIEPPLAPGDSMELKFTTRFEPKGFENEVSWSRIVQNGTFFDNTDVIPMF
                     GYDESKEIGSKNRRKHFDLPEKSRRPELNRADTLHRMEAYIGINSDWVDVETIFRTAP
                     GQIAIAPGSLIKEWEENGRRCFHYRLDHPSFNFYSFLSADYEVTRRDWNGVSLEVYHH
                     KDHAYNVPRMLDALQKSLEYYTQNFGPYYHKQCRIIEFPRFSSFAQAFPGTMPYSEGI
                     GFIQDFKDPDKDIDMVFYVAAHEIGHQYWGHQTCGANMQGSEMLVETFAQWSALMVME
                     REYGRDQMRKFLAYEMDRYLRGRSTETEKELPLATCEGQGYIHYNKGSVQMYQLKEMI
                     GEDKVNAALRALLERYRYQNPPYPVSLDALDEFYAQTPDSLRYIIKDLFEDITLFENR
                     CREAKAKDLGDGRWEVTIKVECAKLKADDLGKETEVKLDDYIEIGAFAKPEKGKTYGK
                     TLHRQRVKVTQKTNTFTFVVNGKPDKAGVDPFSLLIDRNPGDNVKDIE"
     gene            complement(2892..3686)
                     /gene="argB"
                     /locus_tag="PK198_11135"
     CDS             complement(2892..3686)
                     /gene="argB"
                     /locus_tag="PK198_11135"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRF39337.1"
                     /translation="MTTLHIIKIGGNIIDDPAALDAFLRDFAAVEGPKILLHGGGKKA
                     TALSERLGIPTHKHQGRRITDAATLEVAVMVYAGWINKSIVAALSALQCPALGLCGAD
                     ADFITAVKRPVKEIDFGFVGDVVSEKTRGDFLQQLLQQGLTPVVAPITHDGAGQLLNT
                     NADTMAFAVAVAMAARFEVQLIYCFEKKGVLTNVTDDNSVLPHIAWSEYEVLKTAGIV
                     SEGMIPKLDNAFAAIREGVRSVVIGHAGELKEIVAGRAGTRLGGVQ"
     gene            complement(3698..4648)
                     /locus_tag="PK198_11140"
     CDS             complement(3698..4648)
                     /locus_tag="PK198_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HRF39338.1"
                     /translation="MQKFTSVADVSNVEELVTLARDLKSRPQAHHHLGQHKTLGLIFL
                     NPSLRTRLSTQKAGLNLGMNVIVMNMDKEGWALEFQDGAVMNGTTVEHIKDAAAVMGQ
                     YCDILGIRAFPTLKDREEDYGEHLLKKFIRHCKVPVVSLESATLHPLQSLADLITIRE
                     WQPDGRRPKVVLTWAPHIKALPQAVPNSFAEWMCRADLDFTIAHPKGLELAPQFTDGA
                     RILHDQHEALEDADFVYVKNWSAYEPYGQLYSGGEDWLLDNAKIAHTHQAKIMHCLPV
                     RRNVELSDELLDGPQSLILEQANNRTFAAQAVLLQMLAEL"
     gene            complement(4670..6151)
                     /gene="guaB"
                     /locus_tag="PK198_11145"
     CDS             complement(4670..6151)
                     /gene="guaB"
                     /locus_tag="PK198_11145"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRF39339.1"
                     /translation="MEREIAYPEQVLTEALTYDDVLLVPAYSEVLPREVDISSQLTRD
                     IRLNAPIVSAAMDTVTEYRLAIAMARQGGIGIIHKNMSIAAQADQVRSVKRAESGMII
                     DPVTLGSEASVGDALGLMRQFKIGGIPIIDSNNRLIGILTNRDLRFETSLDRPVREIM
                     TSENLVTAPVGTTLEQAREILQRYKIEKLPVVESDQTLVGLITYKDIMKVQDHPYSCK
                     DSLGRLVVGAAVGVTADMVDRVDALVKVGVDVICVDTAHGHSLGVLQAVRNIKQLHPA
                     LQIIGGNVATGEGAKALADAGADGVKVGVGPGSICTTRIVAGVGVPQLSAVMFAAKAL
                     RGTGVPVVADGGIRYAGDIVKALAAGAGTVMAGGLFAGVEEAPGETIIFEGRKFKVYR
                     GMGSLGAMAQGSKDRYFQDVEDDIKKLVPEGIEGRVPYKGTVAEAMLQYIGGLRAGMG
                     YCGALNMEQLQQAKFVKITAAGFAESHPHNVTITKEAPNYSRR"
     gene            complement(6283..>7294)
                     /locus_tag="PK198_11150"
     CDS             complement(6283..>7294)
                     /locus_tag="PK198_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39340.1"
                     /translation="LLGGDLELNKAPGQDARLVGGKITVTDNVPGDLIITGGEVLITD
                     AVTIGGDLIVAGGKVDMNGTVKGNVDAAAGELLMYGPVEGALNVKGGKVVMAARVAGE
                     SKIAAENLEIRSTARFENNVLYWNKNGSADFGAALAEGVKANFNEALKAKYSEWESKS
                     RLFARRITPAVVAYKLASGILLVGLLIAFFGGFFGRIGGSARTEMGKNFGIGLLYILG
                     IPAVIGVAAITIVGLPAAFAVGGVYGASMAVASALTAVVGAYELNTILNKDWSKGRIL
                     LVSVGIFIGLKALAAIPVLGSLGVFVLTAIAFGFLIQAMRGKVKPVEKTPQTDSMVED
                     MV"
     assembly_gap    7295..7442
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7615..8745)
                     /locus_tag="PK198_11155"
     CDS             complement(7615..8745)
                     /locus_tag="PK198_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39341.1"
                     /translation="MKPFDVYPIFDIEPVKAEGSYIWDAQGRQYLDLYGGHAVISIGH
                     SHPHYVRALEEQLRQIAFYSNSVKIPIQNKLADLLGRLSGYEDYALFLVNSGAEANEN
                     ALKLASFHTGRKKVISFHKSFHGRTSLAVAATDDPKIIAPVNSTENILFLPLNDEAAL
                     ERHFSRDVCAVIVEGIQGVGGVQVPTERFLQKIRTLCDQSGAVMILDEVQSGYGRSGQ
                     FFAHQFAGVRPDLITVAKGMGNGYPIGGVLISPRFEARHSMLGTTFGGHYLACAAGIA
                     VLEVIENENLIERAAEMGSYLMSEMTDFAHVREIRGKGLMIGVELDQPCAEVRRRLLV
                     KHRMFTGASSDKNTLRILPALNLGRADANAFLQAFKYVLAAV"
     gene            8824..>10489
                     /locus_tag="PK198_11160"
     CDS             8824..>10489
                     /locus_tag="PK198_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39342.1"
                     /translation="MVKPVRRNIIFALDPSTPSQNKYTIAMKKIFSICCFLLPFLLPA
                     QQPPCTLLKNIETGQDIPIMSYAHSLLQAENKLFFIGTTCTESPEVYILNIGQDSAYL
                     PHNAWLEARYFYWCNVPYPTVDHFPHRYLTAVGNRVFYILMGGQFGVELWTTDGTAEG
                     SGLVRDIFQGSESSSPEQLTAFRNELWFTADDGVNGRELWRSDGTPEGTQMVLDIAPG
                     AAGSRPLHLRTTGDELFFVAATTGDSPWLWRSDGTAEGTIPWLPLAAIPEGYMVESLQ
                     VAGGLLFLVAYNQSAKKANLYVANAQSNTLDLIIEEARASNSLDIPKEFTEWGGIVYF
                     AASTQAHGRELWRTDGTVAGTTLFKDIHPGANGSNPSDLQRFGNKIYFRTKIGDSALL
                     YSTDGTPESTGPVALSPLPTSGINRIKAGDGQLFFTSGDQRELWRTDGIETERIFGPH
                     YQNQWEFMPVAGGSVYFRVAQNGLWKWNGTPGTMAAPISTLGNNDGNSSPTAYFPMGN
                     DIYISTTNALGRAKIWRSNGTPEGTIQAAFVNTQDTLTLSTVFALAD"
BASE COUNT         2299 a         2860 c         2840 g         2332 t
ORIGIN      
        1 aatgaagctt acgtcctcca aaacctgggt gcgccgcccc gcgtacaacc cggcaaaggc
       61 tgggcgacca ccttctcgca actgcgcagc cagctttcta ccgaatggcg cggcatctat
      121 cgcagcgtgg cttttgtgtt gttggccttg ctgggcttgc tcaactgcgt acccaatatg
      181 ttttacgcca atgagagtta cggcacgcat gcactgccgg tgacctacac gatggtggac
      241 atcattcgcg gctcgtttta tctgttcacc attattatta tggtgtattt cagcggggcg
      301 gtggtgtgga aagagcgcat cgcccgtatg aatgacatca ccgatgcgct gccggtcaaa
      361 aactggacca tgatgctggg caagtacctg tcggtattgg gtgtgctgtt tgtgttgcag
      421 gtattggtca tcgccgcggc ggtaatggca cagagcgcca tgggatacca tcgctatgag
      481 ttgtgggtgt acatcaggga gttgctggtg atagatatgc tgggctttgc tgttaccctg
      541 gcgctgagtt ttctggtaca cgccctgtcg cccaatatgt acctcggatt tttcattgta
      601 gtcatattgc tgattgtcaa cagttttgca tggggtgcgc tgaaaattca gagcaatatg
      661 gtgcaatttg gcgccacgcc gtcttacacc ctgtcggatt tttacggtta tcaacccttc
      721 ttcaaagagt tgttttggtt caacagctac tggttgttgt ttggcgtgct gctgctggtc
      781 ggcgcagttt tgctgtggcc ccgcggccgg ttcagcgcct ggcgcgagcg ttggttcaat
      841 gccggattgg agtggaaaca gtaccgggtg ttcggcgtgt cggcattggc nnnnnnnnnn
      901 gtttaaccgc cggctggacc ttttacaaca cgcagatagt caacaggtac gaaagtgtca
      961 aaaaggaaga acgcgcccaa tacgattacg aaatcaaata caaaaaatac agccaacgcc
     1021 ggcagcctcg cgtgtatgcc gcccgctacg acatagacct gcgccccgaa acgcgctctt
     1081 tgcggttgga aggcgttttt aaaacccgca atatttacac acaaagcatt gatacactgt
     1141 gggtcaacac gccctacaac ggcgattttg aactgcgcaa cgaacgcctg cgccccctcc
     1201 tcgacgactc gctgctgcac tggcagatgt acaaaataga gccaccgctg gctcccggcg
     1261 attcgatgga attgaaattt accactcgtt ttgaacccaa aggctttgag aatgaggtga
     1321 gttggtcgcg cattgtgcaa aacggcacct tcttcgacaa caccgacgtc attcccatgt
     1381 tcggctacga tgaaagcaag gaaattggtt ccaaaaaccg ccgcaagcac tttgacctgc
     1441 ccgaaaaaag ccgccgcccc gaactcaacc gcgcggacac cctgcaccgc atggaggctt
     1501 acatcggcat caacagcgac tgggtggacg tggaaaccat cttccgcacc gcccccggtc
     1561 agatcgccat cgcgcccggc tcgctcatca aagagtggga ggaaaacggc cgccgttgtt
     1621 ttcactaccg cctcgatcat ccgtctttca atttctactc cttcctcagc gccgactacg
     1681 aagtgacccg ccgcgactgg aacggcgtca gcctcgaagt gtatcaccac aaagaccacg
     1741 cctacaacgt gccgcgcatg ctggacgccc tgcaaaaatc gctggaatat tacacccaaa
     1801 acttcgggcc gtactaccac aaacagtgcc gtatcattga gtttccgagg ttttcgtcgt
     1861 tcgcccaggc tttccccggc accatgccgt actcggaggg catcgggttt attcaggatt
     1921 ttaaagaccc ggacaaagac atagacatgg tgttttatgt ggccgcgcac gaaatcggac
     1981 accaatactg ggggcaccaa acctgtggcg ccaacatgca gggcagcgaa atgctggtgg
     2041 aaacttttgc ccaatggagt gccctcatgg tgatggaacg cgagtatggc cgcgaccaaa
     2101 tgcgcaagtt tttagcctac gaaatggacc gctacctgcg cggacgaagc accgaaaccg
     2161 aaaaggaact gccgctggcc acctgcgaag ggcagggcta catacactac aacaagggca
     2221 gcgtgcaaat gtaccaactc aaggaaatga tcggggaaga caaggtgaac gcggccttgc
     2281 gggccttgct ggaacgctat cgctaccaaa atccgcctta ccccgtttcg ttggatgcgc
     2341 tggatgagtt ctatgcccaa acgcccgact cgctccgcta catcatcaag gacttgtttg
     2401 aagacatcac cctgtttgaa aaccgctgcc gcgaagccaa agccaaagac ctcggcgatg
     2461 gccgctggga agtgaccatc aaggtggaat gtgccaaact gaaagcggac gatctgggca
     2521 aagaaaccga ggtaaaactg gacgactaca tcgaaatcgg ggctttcgcc aaaccggaaa
     2581 aagggaaaac ctacggcaaa accctgcacc gccaacgcgt gaaagtgacg cagaaaacga
     2641 acacttttac tttcgtggtg aacggaaaac ccgacaaagc cggcgttgat ccgttctcgt
     2701 tgctgataga ccgcaatccg ggcgataatg tgaaggatat agagtagggg agtgtttcgc
     2761 ctggttggaa cggctgctgg ctactggctg caagctacaa gcggcgttcc ccttgcgctt
     2821 agcaccgccc actgcatacc gcctaccgcc cgctctacgc ttagcaccgc ctaccgccga
     2881 cggcaaaccg cctactgcac accgcccaac cgcgtccctg cccgccccgc cacaatctcc
     2941 ttcagttcgc cggcatgccc gatgaccacg ctgcgcaccc cttcccgaat ggccgcgaag
     3001 gcattgtcca acttaggaat catgccctcc gaaacgatgc cggcggtttt gagcacctcg
     3061 tattccgacc acgcgatgtg aggcagcacg gagttgtcgt ctgttacatt ggtcagcacg
     3121 cccttttttt caaaacaata gatcagttgc acctcaaaac gagcggccat ggccaccgcc
     3181 acggcaaagg ccatcgtatc ggcattggtg ttcagcaact ggcccgcgcc atcgtgcgta
     3241 atgggcgcca ctaccggtgt aagcccctgc tggagaagtt gttgcaggaa atcgcctctc
     3301 gttttttccg aaaccacatc acccacgaag ccgaagtcta tttccttcac cgggcgtttt
     3361 accgccgtga tgaagtccgc atcggcgccg cacaaaccca gcgccgggca ttgcagtgcc
     3421 gacagcgccg ccacgatgct tttgttgatc cagccggcat acaccatgac tgctacttcg
     3481 agtgtagcgg cgtcggtgat gcggcgtccc tggtgtttgt gggtgggaat gccgagacgt
     3541 tcggagagcg ccgtggcctt tttgccgccg ccgtgcaaca gaattttggg gccttctacg
     3601 gctgcaaaat cgcggaggaa ggcatccaat gcagcgggat cgtcaatgat gttgccgccg
     3661 attttgatga tgtggagagt tgtcattact gataatttta caactccgcc agcatttgca
     3721 acaacacggc ctgcgcggcg aaagtgcggt tgttggcctg ttcgagaatg agtgattgcg
     3781 ggccatccaa gagttcgtcg ctgagttcca cattgcggcg cacgggcagg cagtgcatga
     3841 ttttggcctg atgggtatgc gctattttgg cattgtccaa gagccagtct tcgccgccgg
     3901 aatacagttg cccgtaaggt tcgtaggccg accagttttt aacatacaca aaatcagcgt
     3961 cttccagggc ctcgtgctga tcgtgcagga tgcgcgcccc gtccgtaaac tgaggcgcca
     4021 attccagccc tttcgggtgt gcgatggtaa aatccagatc ggcacggcac atccattcgg
     4081 cgaaggaatt gggtacagcc tgtggcaggg ctttgatatg cggcgcccag gtgagtacca
     4141 ctttgggccg gcgaccgtcc ggctgccact cgcggatggt gatgaggtcg gccagacttt
     4201 gcagcgggtg cagggtggcc gactcgaggc tgacaaccgg cactttgcaa tgccggatga
     4261 attttttcag caggtgttcg ccatagtctt cctcgcggtc tttgagtgtg gggaaggcgc
     4321 gtatgccgag gatgtcgcaa tattgtccca ttacggcggc ggcgtctttg atgtgttcca
     4381 cggtggttcc gttcatcacg gcgccgtcct gaaattccaa agcccagccc tctttgtcca
     4441 tgttcatcac aatgacgttc atgcccaggt tgaggccggc tttttgggtg ctgaggcggg
     4501 tgcgcaaact ggggttgaga aaaataagcc ccagtgtttt gtgttgcccc agatggtggt
     4561 gcgcctgtgg ccgcgatttg aggtcgcggg ccagtgtgac aagttcttct acgttgctca
     4621 catcggcaac gctggtgaat ttttgcatgg ttttttggat gttatggcat caacgccggc
     4681 tgtagttggg cgcttctttg gtgatggtga cgttgtgcgg gtggctttcg gcaaaaccgg
     4741 cagcggtgat ttttacaaat ttcgcttgct gcaactgctc catattgagc gcgccgcagt
     4801 agcccatgcc cgcccgaagc ccgccgatgt actgcaacat ggcctcggca acggtgcctt
     4861 tgtaaggaac ccgtccttct atgccttctg gaacgagctt tttgatgtcg tcttccacat
     4921 cctgaaaata gcggtctttg gagccttgcg ccatagcgcc caatgatccc atgccgcgat
     4981 ataccttaaa cttcctgcct tcaaaaatga tggtttcgcc cggcgcttct tccactccgg
     5041 caaacaaacc gcccgccatg acggtgcccg ctccggctgc caatgctttc acaatatcgc
     5101 ccgcatagcg gatgccgccg tctgctacga caggaacgcc cgtaccgcgc agtgccttgg
     5161 cggcaaacat caccgccgac aactgcggca cgcccacgcc ggctacaatg cgcgtggtgc
     5221 agatactgcc cggaccaacc cccactttca ccccgtcggc gccggcatcg gccagcgctt
     5281 tggccccctc gccggtagcc acgttgccgc cgatgatctg gagcgcgggg tgcagttgtt
     5341 tgatgttgcg cacggcttgc aacacgccca gcgagtgccc gtgcgccgta tcaacgcaga
     5401 tcacatccac gcccactttc acgagggcat ccacgcggtc caccatgtcg gcggtgacac
     5461 ccactgctgc gcctaccacc aaacggccga ggctgtcttt gcaggagtag gggtggtctt
     5521 gcaccttcat aatgtctttg taagtgatga ggcccacgag cgtctggtcg ctttccacta
     5581 caggcagctt ctcgattttg tagcgctgga ggatttcgcg ggcctgttcc agcgtagtgc
     5641 ctacgggcgc ggtgaccaaa ttttcggagg tcatgatctc gcgcacgggg cggtcaaggc
     5701 tggtttcaaa acgcaggtcg cggttggtga ggatgccgat gagtcggttg ttgctgtcta
     5761 tgatgggaat gccgccgatt ttgaactgcc gcatcaggcc gagcgcatcg cccacggaag
     5821 cctcgctgcc cagcgtaacc gggtcaataa tcataccgct ttcagcgcgc ttgacgctgc
     5881 gtacctgatc ggcctgggcc gcaatgctca tgtttttgtg aataatgccg atgcctccct
     5941 gccgcgccat agcaatagcc aagcggtact cggtgacggt atccatagcc gccgacacga
     6001 tgggcgcatt gagccggata tcgcgggtga gttggctgga gatgtccact tcgcggggaa
     6061 ggacttcgga atacgcaggg acgagcagaa cgtcgtcgta ggtgagcgcc tcggtgagga
     6121 cttgttcggg atatgcaatt tctctttcca tgcgcaaagg tatgcacggg cagggataac
     6181 gggcaaagga ttgggagttt ttttataaaa aaagcccgcc gacaatcggc aggcttcgtt
     6241 tcatttggtt ttttacaaaa tgctcttgtt gttgagcgcg ggttatacca tatcctccac
     6301 catcgagtcg gtttgaggtg ttttttcaac aggttttacc ttgccgcgca tggcctgaat
     6361 gaggaatccg aaagcaatgg ccgtgaggac aaataccccc aagctgccca atacgggaat
     6421 ggctgcgagc gctttgaggc cgatgaaaat gcccacagac accaacagta tgcggccttt
     6481 actccagtct ttattcaaaa tggtattgag ttcatacgcg ccgactaccg ccgtgagcgc
     6541 actcgctacc gccatcgaag cgccatacac gccgccaacg gcaaaggctg ccggaagtcc
     6601 gacaatcgtt atggctgcca ccccgataac tgccggaata ccaagtatgt acaacaagcc
     6661 tatgccgaag tttttgccca tttctgtacg ggcgctgccg cctatgcgtc cgaaaaatcc
     6721 gccgaagaag gcgatcaaca gaccaacgag caatatgccg gaagccaatt tgtaggccac
     6781 aacggcaggc gttatgcggc gggcaaacag gcgggatttg ctttcccatt cggaatattt
     6841 ggctttcagt gcttcgttga aattcgcttt gaccccttct gccagcgctg cgccgaaatc
     6901 ggcagaaccg tttttgttcc aatacaatac gttgttttca aagcgggctg tggagcggat
     6961 ttcgagattt tcggcagcta ttttcgattc gccggccacg cgggcagcca tcactacctt
     7021 gccgcctttc acgttgaggg cgccttctac ggggccatac atcagcagtt cgcccgcagc
     7081 ggcatccaca ttgcctttca ccgtgccgtt catatccact ttgccgccgg ccacgatgag
     7141 gtcgccgccg atggtaacgg catccgtgat gagtacttcg ccgccggtga tgatgaggtc
     7201 gccgggtaca ttgtcggtca cggtgatttt gccgcctaca aggcgcgcat cctggccggg
     7261 cgctttgttg agttccagat cgccacccag cagcnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nngttgagag ttttgtgcaa aagagaagcc cgcgaaggcg agcaggttca tcaagacgag
     7501 tgtccaggaa atgcgtttca tgacttttct gattttggag caaaagtgcg ctccaagctc
     7561 ggcgaaaagc aattttattc gatgaaaccg acggcaatgg cgttcaaacc gacctcaaac
     7621 ggcggccaaa acgtacttga aagcctgcaa aaacgcattg gcatcggcgc ggccgaggtt
     7681 gagcgcaggc aaaatgcgga gggtattttt gtcggaagaa gcgcccgtga acatgcgatg
     7741 tttgaccaac agacggcggc gtacctcggc gcagggctgg tctaattcca ccccgatcat
     7801 caggcccttg ccccgaattt cgcggacgtg cgcaaagtcg gtcatttcgc tcatcaggta
     7861 gctacccatt tcggcggccc gctcaataag gttttcattt tcaataactt ccaacacggc
     7921 aatgcccgca gcacaggcca agtaatgccc cccgaaagta gtgccgagca tgctgtgccg
     7981 cgcctcgaaa cggggcgaaa tgagtacgcc gcctatcggg tacccgttgc ccatgccttt
     8041 ggccaccgtg atgagatcgg gccgcacacc ggcgaattga tgcgcaaaga attgcccgga
     8101 gcgcccgtag cccgattgca cctcatcgag gatcatcacc gcgccggact gatcgcataa
     8161 ggttctgatt ttctggagga agcgctcggt aggaacctgc acgccgccca cgccctgtat
     8221 gccttctacg atgacggcgc acacatcgcg gctgaaatgg cgttcgagcg cggcttcgtc
     8281 gttgagcggc aggaagagga tgttttcggt ggagttgacc ggcgctatga tcttggggtc
     8341 atcggtggcg gcaacggcca gcgaggtgcg cccgtggaag gatttgtgaa acgaaatgac
     8401 ctttttcctc cccgtgtgaa aagaggccaa tttgagcgcg ttttcattgg cttctgcgcc
     8461 ggaattgacc aaaaacagtg cgtagtcttc gtatcccgac aggcggccca gcaagtcggc
     8521 cagcttgttt tggatgggaa ttttgactga gttggaataa aaagcgatct gacgcaactg
     8581 ctcctccaat gcgcgcacat aatgcgggtg gctgtgcccg atggaaatga cggcatggcc
     8641 gccgtagagg tccagatatt ggcgtccctg tgcgtcccag atatacgagc cttcggcctt
     8701 cacaggttct atgtcgaaga tgggatatac gtcgaatggt ttcataaagt gatccgtttt
     8761 gccggcgcat gaactccgcc gacggggcaa aggtaagccg aactgcccga aaaatatgcg
     8821 ggaatggtaa agccggtgcg aaggaatatt atctttgccc tcgacccttc tactccatca
     8881 caaaacaaat acaccattgc catgaaaaaa atcttttcca tctgttgttt tcttcttcct
     8941 ttcctgctgc cggcgcagca accaccttgc acgctactga aaaacattga aacagggcag
     9001 gatattccga taatgagtta tgcgcactca ctgctacagg ctgaaaataa gctttttttt
     9061 attggtacta cctgtaccga atcccctgaa gtatatatac tgaacattgg ccaggattca
     9121 gcctatctgc cccataacgc ctggctcgaa gctcgatatt tctattggtg caatgtccca
     9181 taccccactg ttgaccattt tccccatcgt tatcttaccg cggtaggaaa ccgggttttt
     9241 tacatcctaa tgggcggcca atttggcgtt gaactatgga caacggatgg tacagcggaa
     9301 ggcagcggcc tcgttcggga tatttttcaa ggttcagaat cctccagccc ggagcagtta
     9361 actgctttta gaaatgagct ttggtttacg gccgatgacg gcgtaaatgg ccgcgaacta
     9421 tggcgcagcg acggcacgcc ggaaggcacc caaatggtgc ttgatattgc gcccggtgca
     9481 gcaggctccc gccctttgca tcttcgcaca acaggggatg aattgttttt cgtagctgcc
     9541 acgacgggag acagcccgtg gctttggaga tcggacggca ctgcggaggg cacaattccc
     9601 tggttgccct tggctgccat ccccgaaggg tacatggtcg agagcctcca agtagccggc
     9661 gggctgcttt ttctcgtggc atacaatcaa agcgctaaaa aggccaatct gtatgtagcg
     9721 aatgctcaaa gcaataccct tgatctgata atagaggaag cccgtgcatc gaattcatta
     9781 gacattccca aagagtttac cgaatggggc ggcatagttt attttgcggc ttccacacag
     9841 gctcatgggc gagaactatg gagaacagac gggacggtgg ccggcactac gttgtttaaa
     9901 gacatccatc cgggggccaa cggaagcaat ccttctgatc tccaaagatt tgggaataaa
     9961 atttatttcc gtaccaaaat tggtgattcg gcgctcttat acagtacaga tggtaccccg
    10021 gaaagtaccg ggccggtcgc gctgtctccc ttaccgacaa gtggaataaa cagaatcaaa
    10081 gccggcgatg ggcaattgtt ttttaccagt ggcgaccagc gtgagctatg gcgaaccgat
    10141 ggaatcgaaa ccgaaaggat atttgggcca cattatcaaa atcagtggga gtttatgccg
    10201 gtagcgggcg gcagcgttta tttccgagta gcgcagaatg gtctgtggaa gtggaacggt
    10261 acgccgggta caatggctgc cccgatcagc actttgggaa ataacgacgg taactcctct
    10321 ccgacagcgt attttcctat gggaaatgac atatatatat cgacaactaa cgcgctcgga
    10381 cgtgcgaaaa tatggcgcag caacggtacg ccggaaggca ccatacaggc tgcctttgtc
    10441 aatacacaag ataccttaac tctgagtaca gtgttcgcac tggccgaca
//
LOCUS       DAORLF010000121        10455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3406_length_10455_cov_2.321827, whole genome shotgun sequence.
ACCESSION   DAORLF010000121 DAORLF010000000
VERSION     DAORLF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_10455_cov_2.321827"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1368
                     /locus_tag="PK198_11165"
     CDS             217..1368
                     /locus_tag="PK198_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRF39343.1"
                     /translation="MKLVAKTMAGLEPVLASELESIGAAEIRTAPRAIYFEGDHEVMY
                     RANFELRTPLRILLPIADFQVRNENDLYRHVFDIDWRQYMDVENTFAIDAVVWSETMR
                     HSHFAALKAKDAIADRFRKETGRRPDVNVHEPDLRVHLHISGQTANLSLDSSGESLHR
                     RGYRTRGGMAPLNETLAAGMVMLSGWNGDRPFIDPMCGSGTLLIEAALIAGRIPPQWQ
                     RRHFGFMRWKDFQPELWEKVRRRALLTPDVVWPLIFGSDRDFKAIRTSQDNIAEAHLR
                     GKIKLEKMPFEHLTPPEGPGTLIMNPPYDERIPVEDILPFYKSIGDQLKQRYSGYDVW
                     IISAHLEALKQIGLRPSRKITLFNGPLECKYQLFRMYEGSKRTNSEQSW"
     gene            1462..>1967
                     /locus_tag="PK198_11170"
     CDS             1462..>1967
                     /locus_tag="PK198_11170"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39344.1"
                     /translation="MKKLLLALTLVIASAATLVAQRAITGKVSDQGKEPLIGATVLVD
                     GTSVGTVTDVNGDFSLSVPTGASTLIVSYTGYATLRVTLTSASNYDLTLETDVVALSD
                     VVVVGYGTASRKELTGSVSKINSDAISRLPVTGLDQALQGQAAGVQVTSASGTPGASV
                     SVRVRGPSS"
     assembly_gap    1968..1977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1978..4558
                     /locus_tag="PK198_11175"
     CDS             <1978..4558
                     /locus_tag="PK198_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39345.1"
                     /translation="RGVGSINYSNAPLYVVDGVPINTGSYSQIGVGGQQTNALADLNP
                     ADIESMEILKDAAAAAIYGSRASNGVVLITTKRGKAQKTQINLNSYYGTQSVWRRVEP
                     VSGPEYVSYIQEAVRNRQTPTTLPSNVGLAGLNNDPSTYPTTNWFDEIFRDAPISQTD
                     LSFSGGNERTKFFLSGSYFNQDGTVIGSNFNRYSFRLNLDNLVTDKFKVGVSTGFSRS
                     HSNRIQNDNNIYGVVSTALLLGSHIPVRNADGTYGRDPNASIENPVANALEPSYDVYS
                     NRLLTNIFGELDIVEGLKFRTSFSVDYLNVRDDRFIPATHISAAGVNGRGEQGSSTDL
                     NLVNENYFTYRKSFGDLNFDALVGASFQNSKYEDFFGRGENYPGNTIRELAAASVKQT
                     VTSSKSEWGLNSYFSRFNLNWQQKYFISASVRADGSSRFGANNRWGVFPAVSGAWRIG
                     QESFLRDNPVISELKLKGSWGIRGNQNINNFASRALISPGANYLQRAGLAPSQLGNPN
                     LTWEEREDIDLGLELGLFRDRIVLTVDAYQGTTSELLLSRPLVGASGYTGITENIGAV
                     RNKGIDIGLNTTNVTTKNFEWTTNLNLSFFDNEILKLAGAPFAAGFASWVAEGEALGA
                     FRGYQVERLFQTQQEIDDLNAKAREMTGSASAVYQSTLTRPGDIMFRDINGDGRITAD
                     DQTILGDANPDFYGGITNNLSGFGFDLSFFFQFSVGNYVYNNTRSFSEGMNGVFGQAS
                     TILNRWTPDNPTTDTRYPRAVFQDPNNNRRVSDRFLEDASYLRLKNLSLGYTIPSDVM
                     KKIGMTRARIYVSGQNLLTFTKYSGFDPEVSTFAETNTAPGTDFLTFPQARTMLVGLQ
                     LGF"
     gene            4603..5958
                     /locus_tag="PK198_11180"
     CDS             4603..5958
                     /locus_tag="PK198_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF39346.1"
                     /translation="MKNIFKIFAFLAILGLIPACEGNLEIEPYNSLDASTGFKTKQDV
                     DAALLGGYSAIQNSNYYGLQMQVLPDLYADILSHTGTFPTYAQVANRQLLADNTSLTS
                     LWNQVYVAIDRTNNVIASAPGVSDPSFNVNAALGEARLLRAFHYFNLLRLYGGMPEGF
                     NKGNGLGLPLYTTPTLSAEDAAPKPRASETETWALIMEDINFAVANLLSTKVNGRVNK
                     FTAQALKARAHLYRGEWAEAEAAANDVITGGGYTLMPTATYANIWLSQNTTEAIWELQ
                     FDVNNTNSIAFWYYPTANGGRNEFTSLASFRDAHEDGDVRKVVNVSTAPANKTQKYTR
                     ISGTDNVMLIRLAEMYLTRAEARAQQNKLSDAIADLNVIRNRAGLPNANPATVAEAVD
                     AILKERRFELAHEGHRWFDMRRVGRWNAFGLTESFRSRWPLPQREVQTSGDIIAQNPG
                     Y"
     gene            6118..6528
                     /locus_tag="PK198_11185"
     CDS             6118..6528
                     /locus_tag="PK198_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39347.1"
                     /translation="MKYLLFSGTVFVVLLAACGTPKQGSQSNVVAEKSYNCVGTEPFW
                     NVKVEASGITFLLMGEEAVVYPYKAARKEGAAQVYETAVQGSRLKLSISEEGCSDGMS
                     DTNYPYKVTVEKDGTTYQGCAFLLGKNPMRGEER"
     gene            6660..6730
                     /locus_tag="PK198_11190"
     tRNA            6660..6730
                     /locus_tag="PK198_11190"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6692..6694,aa:Gln,seq:ttg)
     gene            complement(6792..>7000)
                     /locus_tag="PK198_11195"
     CDS             complement(6792..>7000)
                     /locus_tag="PK198_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRF39348.1"
                     /translation="ISWYDFAIAIKEVSGSDCIVKPIPTSQYPTPARRPAFSVLDTQK
                     VQDVYGVEMKNWKESLKVCLRNLL"
     assembly_gap    7001..7017
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7018..>7251)
                     /locus_tag="PK198_11200"
     CDS             complement(<7018..>7251)
                     /locus_tag="PK198_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRF39349.1"
                     /translation="KGEEEALRYNPDSIIIRTAWVYSEYGKNFVRTMLQLMKEKESIN
                     VVHDQVGAPTYAYDLAVLFLHIITSGKWQPGIY"
     assembly_gap    7252..7263
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7264..7644)
                     /locus_tag="PK198_11205"
     CDS             complement(<7264..7644)
                     /locus_tag="PK198_11205"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRF39350.1"
                     /translation="MMNKKPVILVTGANGQLGKELKKIAPTASALDFVFLSREDMPIH
                     HFEMVRHYFKVYQPAFLINCAAYTAVDRAEQEKDKAFQINAEAVGVLAAVCKENHCRF
                     IHISTDYVFDGKSQTPYQEDAKTNP"
     gene            complement(7641..8201)
                     /gene="rfbC"
                     /locus_tag="PK198_11210"
     CDS             complement(7641..8201)
                     /gene="rfbC"
                     /locus_tag="PK198_11210"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRF39351.1"
                     /translation="MPFTETEIPGLLIFEPDVFEDDRGYFFESYNESKFNRQGIFVQW
                     VQDNQSSSQSGVIRGLHFQRPPFAQTKLVRVLKGKILDVVVDIRKGSPTFGKSFSLVL
                     SGKNKKQLLIPKGFAHGFSVLSDKAEVLYKCDALYNKESEAGILYNDPQLNIDWGVPD
                     DMIIVSEKDKTLPLFKDCTAPFEYNG"
     gene            complement(8203..>8824)
                     /locus_tag="PK198_11215"
     CDS             complement(8203..>8824)
                     /locus_tag="PK198_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRF39352.1"
                     /translation="MNELSIIFNLIGINTYEVLEAAGTKWNFLKFQPGLVGGHCIGVD
                     PYYLTYKASELGYDAEVILAGRNINDNMAKYVAKSVIQHIIKNSSDVKNAKVLVMGAT
                     FKENVSDIRNSKVADVVNALKSYHLNVDVVDPHADSDELNHEYGFSLSKNTDTDYDAV
                     VVTVPHNEYKALGDDYFASITKAHAMIADLKGIYRNIIIKRNYWSL"
     assembly_gap    8825..8848
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8849..9500)
                     /locus_tag="PK198_11220"
     CDS             complement(<8849..9500)
                     /locus_tag="PK198_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF39353.1"
                     /translation="MYQDLLDKKAKLAVIGLGYVGLPIALEFAKKISVIGFDINEKRV
                     ELMKQGIDPSNELEKSDFDGCDIVFTNSLDVLREAKFFIVAVPTPVDEHNVPDLTPVK
                     KASETIGKVIKKGDYVVFESTVYPGCTEEDCLPVIEQLSGLKNVTDFKSGYSPERINP
                     GDKTHTLAKIIKVVSGCDAESLEEIAKTYELVVAAGVHKASSIKVAEAAKIIENTQR"
     gene            complement(9522..>9982)
                     /locus_tag="PK198_11225"
     CDS             complement(9522..>9982)
                     /locus_tag="PK198_11225"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF39354.1"
                     /translation="FNTYGPRMRLNDGRALPAFIGQALRGEDLTVFGDGSQTRSFCFV
                     DDLIDGIYKLLLSDYKMPVNIGNPNEISLKDFAEEILSLTGNKVKIIYQPLPVDDPKQ
                     RQPDISKAKQLLGWEPKVNRKEGLKITYDYFKSLPQEELYKQPKEFKSLK"
     assembly_gap    9983..10071
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10072..>10455)
                     /locus_tag="PK198_11230"
     CDS             complement(<10072..>10455)
                     /locus_tag="PK198_11230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF39355.1"
                     /translation="DKFIREGFAVIGMDNLITGDLKNIEHLFKLEQFEFYHHDVTKFI
                     HIPGELDYIMHFASPASPIDYLKIPIQTLKVGAMGTHNCLGLAKAKNARMLVASTSEV
                     YGDPLVHPQTEEYWGNVNPVGPRGV"
BASE COUNT         2603 a         2660 c         2302 g         2738 t
ORIGIN      
        1 cgaccacgcc acaggcgtgg ttcgagtcac gagtttgatt ttccaaaata aaaaagacct
       61 taattccaac aggagattgt gaaatttccc ctaatggaaa acctaatttt tcaactagta
      121 aacaaactca tagctcgtgc cacgcctcgg cgtggtcgta gcttttgggt accggctgtg
      181 tagttacgtt ttgatgaatc tgaatgtcat gcctgtatga aacttgtagc aaaaacgatg
      241 gccggcctcg aaccggtact tgcttccgaa ttagagagca tcggcgctgc cgaaatacgc
      301 accgcaccgc gcgcaatata ctttgagggc gaccatgagg tgatgtaccg cgccaacttt
      361 gagttgcgaa cgcccctgcg catcctgctg ccgattgccg attttcaggt gcgcaacgaa
      421 aacgacctgt accgccatgt ttttgatatt gactggcggc agtacatgga cgtagaaaac
      481 acctttgcca tagatgccgt ggtgtggtcg gaaacgatgc gccactcgca ctttgccgcc
      541 ctcaaagcca aagatgccat tgctgaccgc tttcgcaaag aaaccggccg ccgccccgat
      601 gtgaatgtac acgaacccga tttgcgcgtc cacttgcaca tcagcgggca aacggccaac
      661 ctctctttgg acagttccgg cgagtccttg caccgccggg gctaccgcac gcgtggaggc
      721 atggctccgc tcaacgaaac gctggccgcc ggcatggtca tgctttccgg ctggaacggc
      781 gaccgcccgt ttatagaccc catgtgcggc tccggtacgc tgctcattga ggctgcgctc
      841 attgcaggcc gcatcccgcc gcagtggcaa cgccggcatt tcgggttcat gcgttggaaa
      901 gattttcagc ccgaactctg ggaaaaagtg cgccgccggg ccttgctgac ccccgatgtg
      961 gtgtggccgc ttattttcgg ctccgaccgc gatttcaaag ccatccgcac ttcgcaggac
     1021 aacatcgcag aggcgcatct gagaggcaaa ataaagctgg aaaaaatgcc ttttgaacac
     1081 ctcacgccgc ccgaaggccc cggcaccctc attatgaacc cgccctacga cgagcgcatt
     1141 cccgtagagg acatactccc attttacaaa agcatcggcg accaactcaa gcagcgctac
     1201 tccggctacg acgtttggat catatccgcg catctggaag cactcaagca gatcgggctt
     1261 cggccttccc gaaaaatcac cttatttaat gggcctttag agtgcaaata ccagcttttt
     1321 cggatgtatg aggggagtaa gcgaacaaat tctgaacaga gttggtgatt tgatgttaac
     1381 tcccggttaa gttttttgca tagctttgca atggctttac attcatacca atttagtttt
     1441 actaacaaaa atgtgttcct tatgaaaaaa cttttgcttg cactcacgct ggtgatcgca
     1501 tctgctgcaa cgctcgttgc acagcgggcc atcaccggaa aagtgtctga tcagggcaag
     1561 gagccgctga tcggcgctac cgtattggtg gacggcacct ccgtaggtac cgtcaccgat
     1621 gtgaacgggg actttagcct gtctgtaccg acaggggcaa gtaccctcat cgtcagctac
     1681 acgggctacg ctacccttcg tgtgacgctg acttctgcct ccaactacga cctcacatta
     1741 gaaaccgatg tggtggcttt gtccgacgta gtggtggtag gttatggaac tgcctctcga
     1801 aaggaattga ctggttccgt ttcgaagatc aatagcgacg ctatttcgcg tctgccggta
     1861 acaggtctgg accaagcgtt gcagggccag gccgcaggtg tgcaggtgac ctctgcgtca
     1921 ggtacgccgg gcgcttctgt gtcggtgcgt gtgcgtgggc catcgtcnnn nnnnnnnacg
     1981 gggagtgggg tcgatcaact actccaatgc gccgctgtat gtagtggacg gcgtgcccat
     2041 caacaccggc agctactcgc aaatcggcgt aggcggtcag cagaccaacg cattggccga
     2101 cctgaaccct gccgacattg agtctatgga gattctgaaa gatgcagcag cagcagccat
     2161 ttacggctct cgtgccagca acggtgtggt actcatcact accaagcgcg gcaaagctca
     2221 aaaaacgcag atcaacctga actcctatta cggcacacaa agcgtatggc gccgagtgga
     2281 gccggtttcg gggccggagt atgtatctta cattcaggag gcagtgcgca accgccagac
     2341 cccgactacc ttgcccagca atgtcggtct ggcaggcttg aacaacgatc cgagtactta
     2401 tccgactacc aactggtttg acgagatttt tcgagatgcg cccatttctc aaaccgacct
     2461 gagcttctcc ggcggcaacg agcgcaccaa gttcttcctc tccggctcat atttcaatca
     2521 ggacggcacg gtgatcggtt ccaacttcaa ccgttacagc ttccgtttga atttggataa
     2581 tctggtaaca gacaagttca aagtcggcgt cagcacgggc ttcagccgct cgcacagcaa
     2641 tcgcatccaa aacgacaaca acatctacgg tgtagtgagt acggcccttt tgctgggttc
     2701 tcacattccg gtacgcaacg ccgacggcac ctatggccgc gaccccaacg catctatcga
     2761 aaacccggta gccaacgcgc tggaacccag ctacgacgtg tacagcaacc gcttgctgac
     2821 caatatattt ggtgaattgg acatcgtgga gggcttgaaa ttccgcactt ctttcagcgt
     2881 ggattacctt aatgtacgcg acgaccgctt cattccggcc acgcacattt cggcggcagg
     2941 tgtgaacggc cgcggcgagc aaggcagtag caccgacctc aacttggtca acgaaaacta
     3001 tttcacttat cgcaaatcct tcggcgacct caacttcgac gctttggtgg gcgcatcttt
     3061 ccaaaacagc aagtacgaag acttcttcgg tcgtggcgag aactatccgg gcaacacgat
     3121 tagagaattg gccgctgcat ctgtaaaaca aacggttact tccagcaaat cagagtgggg
     3181 actgaacagc tacttctccc gcttcaatct caactggcaa cagaagtact tcatctccgc
     3241 cagcgtgcgt gccgacggtt cttcccgttt cggcgccaac aaccgctggg gtgtattccc
     3301 ggctgtttcg ggtgcatggc gtattggtca ggagagcttc ctgcgcgaca accctgtcat
     3361 ctctgagttg aaacttaaag gtagttgggg cattcgcggc aaccaaaaca tcaacaactt
     3421 cgcctcccgc gccctcatca gccccggcgc caattacttg cagcgcgccg gcttggcccc
     3481 ctctcagttg ggcaacccga acctgacctg ggaagagcgc gaggacattg acctcggtct
     3541 tgaactgggc ttgttcagag accgcatcgt actcacagtg gatgcttacc agggtactac
     3601 gagtgagttg ttgctcagcc gcccgctcgt aggcgcttcc ggttataccg gcatcactga
     3661 aaatatcggc gctgtgcgaa acaaaggtat tgacatcggt ctcaatacga ccaacgtaac
     3721 gaccaaaaat tttgaatgga ctaccaactt gaatctttct ttctttgata atgaaatact
     3781 caaactcgcc ggcgctccat ttgcagcagg ttttgcttcc tgggtggcag aaggcgaggc
     3841 attgggtgct ttccgaggct atcaagtaga gcgtttgttc cagacacagc aggaaattga
     3901 cgacctgaat gccaaagcgc gcgaaatgac gggaagcgcg tcggctgttt atcagtccac
     3961 cttaactcgc cccggcgaca tcatgttccg tgatatcaac ggcgacggac gcatcaccgc
     4021 cgacgaccag accattttgg gtgatgccaa cccggatttc tacggcggta ttactaacaa
     4081 cctctctggt ttcggtttcg acctgagctt cttcttccag ttcagcgtag gcaactatgt
     4141 ttacaacaat actcgctcct tctcagaggg tatgaacggt gtgtttggtc aggcttccac
     4201 catcctcaac cgctggacgc cggacaatcc gacgaccgac acgcgttacc cgcgcgctgt
     4261 attccaggac ccgaacaaca accgccgcgt ttccgaccgc ttcctcgaag atgcttctta
     4321 cctgcgtctg aaaaacctgt ctttgggcta caccattccc tctgatgtga tgaaaaaaat
     4381 cggaatgacc agagcgcgta tctatgtgag cggccagaac ctgctcacct tcaccaagta
     4441 ctccggcttc gaccccgaag taagtacttt tgctgaaacc aatacggctc ccggcaccga
     4501 cttcctcacc ttcccgcagg cgcgtaccat gttggtaggc ttgcagcttg gattctaaag
     4561 gcagtgttcg gatttaattt ttgaactcaa atttcaattg caatgaaaaa catctttaaa
     4621 atatttgctt ttctggccat actgggcttg atcccggcct gcgaaggcaa tctcgaaatt
     4681 gagccgtata actcgctcga tgcttctacc ggcttcaaaa ccaaacaaga cgtggatgcc
     4741 gcattgttgg gaggttacag cgccatacaa aacagcaact actacggcct gcaaatgcag
     4801 gtgttgcctg atttatatgc cgacatcctc agccacaccg gtactttccc gacatacgcg
     4861 caggtggcca accgccagtt gctggccgac aacaccagcc tcaccagctt gtggaatcag
     4921 gtctatgtgg ccattgaccg caccaataat gtgatcgctt cggcgcctgg cgtttccgat
     4981 ccgagcttca atgtaaatgc tgcgttgggc gaggctcgct tgctgcgcgc attccactac
     5041 ttcaacctgc ttcgcctcta tggcggcatg cctgaagggt tcaacaaagg caatggcctt
     5101 ggcctgcctc tttacaccac gcctacgctg tcggcagaag atgcagctcc caaacctcgc
     5161 gccagcgaga ccgaaacctg ggcgctcatc atggaggaca tcaacttcgc ggtggccaat
     5221 ttactttcta ctaaagtgaa cggtcgtgta aacaaattca cggcacaggc gctcaaggcc
     5281 cgcgctcacc tctatcgcgg cgagtgggcc gaggccgagg cggcggccaa tgatgtcatc
     5341 accggtggcg gttatacgct catgccgacg gctacctacg ccaatatctg gctgagccaa
     5401 aacacgaccg aagccatttg ggagcttcag ttcgatgtga acaacaccaa ctccatcgct
     5461 ttctggtact atcctacggc caatggcggt cgcaacgagt ttacctctct ggcttccttc
     5521 cgcgacgcac atgaagacgg cgatgtgcgc aaagttgtaa atgtcagcac tgcgcctgcc
     5581 aacaaaacgc agaaatacac gcgcatcagc ggcactgaca atgtgatgct cattcgcctg
     5641 gcggaaatgt acctgacccg tgcagaagcg cgcgcgcagc aaaacaagct cagcgacgct
     5701 attgccgacc tcaatgtgat ccgcaaccgc gcaggtttgc ccaatgccaa cccggctacg
     5761 gtagccgagg cagtagatgc tatcctgaaa gagcgccgct tcgagctggc ccatgaagga
     5821 caccgctggt tcgatatgcg ccgtgtaggc cgctggaatg cttttggtct caccgagtca
     5881 ttccgctcgc gctggccctt gccgcagcgc gaagtgcaaa cttctggcga catcattgct
     5941 cagaatccag gctattaatt acttaggcca tttttagccg ccaccaaaac agagccgctt
     6001 cgggaaaccg gggcggcttt gttgttgttg aggagtgagc atgattccct ccattttttc
     6061 aaaatccgag atgttgcgta agcgcttctc ctttttaaat aaaaaccatt cagaccaatg
     6121 aaatacctac tgttttccgg cactgttttt gtcgttttac tcgcagcttg cggcacgcca
     6181 aaacaaggca gtcagtccaa tgtagtggcc gaaaaaagct ataactgtgt cggcaccgag
     6241 cctttctgga atgtaaaagt ggaggcttca ggcatcactt ttctgctcat gggggaagaa
     6301 gccgtagtgt atccctacaa agccgcacgc aaagaggggg ccgcacaggt atatgaaact
     6361 gccgtgcagg gcagccgcct gaaattgagc atctccgaag aaggctgttc cgacggcatg
     6421 tccgatacga actatcccta taaagtgacg gtggaaaaag acggcacgac atatcaggga
     6481 tgcgcttttt tgctgggaaa gaacccaatg aggggagagg aacgataatg ttacactgta
     6541 cttagcgggg cgcagccggg gcggataaaa aatttttttc gcagaaactt tttagaattt
     6601 caaagaggtt gtatctttgc acgcgtttcc gaaagagggg gcgttttttc cttccccata
     6661 gacccatggt gtaacggtag cactacagat tttggttctg tctgttaagg ttcgaatcct
     6721 tatgggtcaa cagataaatc ccggtaattc gggatttttt tatgccgcta catttcgctc
     6781 tgcaattttt attacaataa atttctaagg caaactttca ggctttcttt ccaatttttc
     6841 atctcaactc cataaacatc ttgtactttt tgtgtatcaa gaacagaaaa agcaggccgt
     6901 ctggccggag ttggatactg tgaagttgga atgggtttta caatgcaatc gcttcctgaa
     6961 acttctttta tagcaattgc aaaatcgtac cagcttattt nnnnnnnnnn nnnnnnngat
     7021 agattccggg ttgccatttg ccggaagtaa tgatatgcaa aaaaagaaca gccaaatcat
     7081 aagcataagt aggtgccccc acctgatcat gcactacatt aattgattct ttttctttca
     7141 tcagttgcag catcgttctt acgaaatttt ttccatactc gctataaacc caggcggtgc
     7201 gaatgatgat ggaatcagga ttatagcgta aagcctcctc ctctcctttc annnnnnnnn
     7261 nnntggattt gtttttgcat cctcttgata aggcgtctgg cttttaccat caaatacata
     7321 atcggtagag atatgaataa acctgcaatg attctctttg catactgccg caagcactcc
     7381 taccgcttca gcgtttattt gaaatgcttt atccttttcc tgttcggccc tgtccacagc
     7441 agtataggct gcacaattaa ttaaaaaagc aggttgataa actttgaaat aatgcctcac
     7501 catttcgaaa tgatgaatgg gcatatcttc tcttgacaaa aagacaaaat ctaatgccga
     7561 agctgtaggt gcaatttttt tcaactcttt tccaagttgt ccattggcac ctgtaacaag
     7621 tattacgggc tttttattca tcatccattg tattcaaatg gagcagtaca atccttaaac
     7681 aaaggaaggg ttttatcttt ttcagaaaca atgatcatat catcaggcac gccccagtca
     7741 atatttaact gcggatcatt ataaagaatt ccggcctcgc tttctttatt ataaagtgca
     7801 tcgcacttat acaatacttc ggccttatcg ctcagtaccg aaaaaccatg tgcaaaaccc
     7861 tttggaatca ataattgctt cttatttttt ccggaaagaa ccagactaaa agattttccg
     7921 aatgtgggtg atccttttct gatatcaaca accacatcca gaatttttcc tttcaataca
     7981 cgcaccaatt ttgtttgtgc aaatggtggt cgttgaaaat gcaaacctct gattacgccg
     8041 ctttgggatg atgattgatt atcttgtacc cattgtacaa aaatgccctg ccggttaaat
     8101 tttgattcat tgtatgactc aaaaaaatat cccctgtcat cttcaaacac atcaggttca
     8161 aaaatcaata agccgggtat ttctgtttca gtaaatggca tattataaac tccagtagtt
     8221 tcttttgata attatatttc tataaatccc tttcaaatcg gcaatcatag catgtgcttt
     8281 ggtgatggaa gcaaaataat catcgcccaa tgctttgtat tcattatgcg gcaccgtcac
     8341 cactactgca tcatagtcag tgtccgtatt tttggaaagc gaaaaaccat attcatgatt
     8401 cagttcatcg gaatcggcat gaggatctac cacatccaca ttcaggtgat atgattttaa
     8461 tgcattgacc acatctgcta cttttgaatt ccgaatatcg cttacattct ctttaaaggt
     8521 agcacccatc accagcactt ttgcgttctt tacatcactt gagtttttaa taatatgttg
     8581 aataactgac ttggcaacat attttgccat attatcattt atgtttctgc ctgccagtat
     8641 tacttcagca tcatagccca gctcgctggc tttataagta agataataag gatctacacc
     8701 gatgcaatgc ccgccaacca atcccggttg aaatttcaga aaattccatt tggtgcctgc
     8761 ggcttccaat acttcataag tatttattcc aatcaggtta aaaataattg acaactcatt
     8821 cattnnnnnn nnnnnnnnnn nnnnnnnnca cgttgtgtat tctcaataat ttttgccgct
     8881 tctgctactt taatggagga tgctttatgt acaccggcag ctactaccag ttcataggtt
     8941 ttggcaatct cttctagcga ttcggcatcg cagcctgaaa ccactttaat aatttttgca
     9001 agtgtatgtg ttttgtcacc cgggttaatt ctttccggcg aatagcctga tttaaaatca
     9061 gtaacatttt ttaagcctga aagttgctct ataacaggca agcaatcctc ttcggtacaa
     9121 ccgggataaa ccgtagattc aaatacaaca taatctcctt tcttgattac ttttcctatt
     9181 gtttctgatg cttttttaac cggcgtaaga tcgggcacgt tgtgctcatc taccggtgtg
     9241 ggtacggcta ctataaaaaa ttttgcttct cgcaatacat ccagtgaatt tgtaaacacg
     9301 atatcgcatc catcaaagtc gcttttctcc agttcattgc ttgggtctat accctgcttc
     9361 atgagttcta ctcttttttc atttatatca aatccaatta ctgaaatttt ctttgcaaac
     9421 tccaaagcaa tgggaagacc aacatacccc aatccgatta ccgctagttt cgctttctta
     9481 tctaataaat cctgatacat gatttttttt aatatttatt tttattttaa acttttaaat
     9541 tctttaggct gtttatacaa ctcttcttgt ggcagcgatt taaaatagtc gtaagtaatt
     9601 ttcagccctt cctttctgtt cacttttggc tcccagccca atagttgttt ggccttgctt
     9661 atgtcaggtt gcctttgttt tggatcatcc accggcaatg gctggtaaat gatttttact
     9721 ttattaccgg tgagtgataa aatttcttca gcaaaatctt tgagtgaaat ctcgttggga
     9781 tttccaatat tcaccggcat tttataatcg ctcaataata atttataaat accatcaatt
     9841 aaatcatcaa caaaacagaa acttcttgtt tgcgagccat cgccgaatac agttaaatct
     9901 tcgccgcgca atgcctgacc aataaatgca ggcaaagccc tgccgtcatt caatcgcata
     9961 cgaggaccgt atgtattaaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natacacccc
    10081 gagggcctac cggatttaca ttgccccagt attcctcagt ttgtggatgt actaatggat
    10141 ctccatatac ttcgctggtg ctggctacta acattcttgc attttttgct ttggccaatc
    10201 ccagacaatt atgcgtaccc atggctccta cttttaaagt ctgaatagga atttttaaat
    10261 agtcaatagg gctggcagga gatgcaaagt gcatgatgta atctaactcg ccgggtatat
    10321 ggataaactt agtaacatca tggtggtaaa actcaaattg ttctaattta aaaaggtgtt
    10381 caatattttt aagatcgccg gtgatcaggt tatccatacc tataacagcg aaaccttctc
    10441 tgataaattt atcac
//
LOCUS       DAORLF010000122        10455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3407_length_10455_cov_1.967788, whole genome shotgun sequence.
ACCESSION   DAORLF010000122 DAORLF010000000
VERSION     DAORLF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_10455_cov_1.967788"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK198_11235"
     CDS             <1..468
                     /locus_tag="PK198_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39356.1"
                     /translation="LDAGGQNEAVHWGQMVLRSMVGHAGQYVVQSPQSLFGTNQGVSK
                     IIESTGVILLDAILDDNSDQIVFKNALSPDTLDRLTRATLDVVAQHPAVLHGGRGIKE
                     IIAGVAGAVKDKSILERVSCPNSPALCWSNRRDISICCGEKRPPGPSTCWYRQ"
     gene            417..>1332
                     /locus_tag="PK198_11240"
     CDS             417..>1332
                     /locus_tag="PK198_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39357.1"
                     /translation="MWRETPTGAEHLLVSAVKQVMGTLAAQTTDGKPWKPALTNTQML
                     SIVEELVDEVVQNPAWILDEVQGKPVLSEMLDATFASMRSLPKDARLQGEVLRSILKQ
                     NVEITLLQRSVLDKIKWGTEQEEKAILSKALEMVFTFVFPKDARPEIGRLQLLAELLE
                     YVTQTLLRQHPDEKGLVLLDLILFKGGVDFSRGFDKKLTDEILNSVSAALAAHPELLV
                     KGRLLQSIISDLAQTVQDSGIRQPGLLPELIRITLENVASHLEAVADEHAGQPKHILV
                     LAIGQVLRAISQPSEDGRWVPKLSNDQIQ"
     assembly_gap    1333..1346
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1347..1821
                     /locus_tag="PK198_11245"
     CDS             <1347..1821
                     /locus_tag="PK198_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39358.1"
                     /translation="VVLENPQWVRGGDKLFQLIDAVICAMEKLPDFVAVTYTLLRDLI
                     EIALEAVERQGQLLNDIKTQTGETIIRLRFALEELFAILSEAETAEELAWYLSQRQIL
                     NILMEYFLTYIVTIQAKEDTLKTAVSQMKAAVATWRNDTSKTLEEIFGKLGQQSA"
     gene            1851..2711
                     /locus_tag="PK198_11250"
     CDS             1851..2711
                     /locus_tag="PK198_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39359.1"
                     /translation="MKKIARFTTGLAALVLLLALSSCSHLMYLDSAQQNFNRAAALDN
                     EMRFSPRSEVPASPSLYYSTAHAFAEKALQNKSALKKDDVLANAYSIKALSEWKLYRY
                     DEAKATSRLALLEYQALEEDKKLFMPRDKALMEALPSLTDLDQGKRFLYTFFAGTMPG
                     YEQAKDHYFSNVFHPDADKSPKLETAVSKIDMVRSQLEGNNDLALYFVSSQLAGLKTW
                     SDALDFLRQSANKDNSLSDSAKQEARAFVAEQYKLHIKPQKEKLLADLSRLAPKPEAE
                     RMKSYWDDLF"
     gene            2837..4150
                     /gene="ahcY"
                     /locus_tag="PK198_11255"
     CDS             2837..4150
                     /gene="ahcY"
                     /locus_tag="PK198_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRF39360.1"
                     /translation="MHAVANQLKYKVKDISLADWGRKEIELAEAEMPGLMALREQYGA
                     LKPLKGARIAGCLHMTIQTAVLIETLVELGAEVSWSSCNIFSTQDHAAAAIAAAGIPV
                     YAWKGQNEEEFWWCIEQTLYAFEGGEPLNMILDDGGDLTNLVLDQRPELIAGVRGISE
                     ETTTGVHRLYERVTNGTLPMPAINVNDSVTKSKFDNKYGCKESAVDAIRRATDIMMAG
                     KVAVVAGYGDVGKGTAASLRGAGCRVIVTEIDPICALQAAMDGFEVKRMVNAIPRADI
                     VITATGNRDIVTEEHFYLMKDKAIVCNIGHFDNEIDMSWLNQSFGKTKVTIKPQVDKY
                     TLNGKDIIVLAEGRLVNLGCATGHPSFVMSNSFTNQVLAQIELWKHSDRYENAVYTLP
                     KHLDEQVARLHLSKIGVELEELNEDQASYIGVKKEGPFKPEYYRY"
     gene            complement(4225..6696)
                     /gene="pbpC"
                     /locus_tag="PK198_11260"
     CDS             complement(4225..6696)
                     /gene="pbpC"
                     /locus_tag="PK198_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRF39361.1"
                     /translation="MKTAINISGRFFRQKIADFPKAVQLFASRFFTFSLRHKKWLFPL
                     YGLFLLWLFCLPSPLFQDPVSVVLEDRSGELLGAKIAADGQWRFPALDTLSSERYEKA
                     LIEFEDKRFYRHPGVDVLAFGRALRQNISGGRIVSGGSTITMQVIRMARKNKPRSLFN
                     KLTEAFMATRLELGKSKRSILALYASHAPFGGNVVGLDAASWRYFGKSPELLSWAEAA
                     TLAVLPNSPALIHPGRNRNALMEKRNRLLDRLFQRGIIDETSCRLAKEEPLPDKPHPL
                     PREAPHLLDRACAQYARPKSPGPARVHSTLRAELQKQVNEIAIRRHSILTANGIHNMA
                     ILVLDVSKGEVLAYLGNVPDAGAEHGEQVDIVTAPRSSGSILKPLLYTLALQEGRILP
                     GSLLSDVPVQLRDFKPENFGKQYEGVIPARRALSRSLNVPFVHLLQDYGVERFHFALN
                     KLQFSTITKPPTHYGLSLILGGAETTLWDVTNCYASMARMLEHFPEYNSRYNASDFRH
                     CTWLLAPSDNHSSGRLRREPTHLNAGAAWLALEAMRQLERPSEEGDWESFGSGRPVAW
                     KTGTSFGFRDAWAVGVTPHYAVGVWVGNADGEGRPGLIGVQAAAPVLFDVFQLLRTGR
                     WFYPPYDEMIRLPVCAQSGYQPLSICPRDSVWAPRSAVNTPACPYHETIHLDAGKKWR
                     VHADCESPTAIVHQPWFVLPPLEEFFYIAKHADYQPLPPLRPDCEVKDARLPMQLIYP
                     RRAARIYVPVNLDGSVTGTVFTVAHRNASKKIYWHLDEEFLGTTENYHNMTLQPAPGT
                     HQLTLVDEDGYRLVQTFEIVAGKKR"
     gene            complement(6718..>6916)
                     /locus_tag="PK198_11265"
     CDS             complement(6718..>6916)
                     /locus_tag="PK198_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39362.1"
                     /translation="NLLIINLNGEETTGYWKISGDQILMQVTKTSAPPYDILEMTPQK
                     IVLRPDSNDPFIFSRVMVPAK"
     assembly_gap    6917..6926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7247..7330
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7331..8104)
                     /locus_tag="PK198_11270"
     CDS             complement(<7331..8104)
                     /locus_tag="PK198_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF39363.1"
                     /translation="MKATLEKIEPVFGNSFTIRKFTDINYLGKPYWHFHPEYEIVYIS
                     NGRGKRHIGEHVSFYEHGDLIFLGPNMPHFGFSEELYDEHVEIVVQMREDFLGAEFLS
                     KPEMNAIRLLFERSHQGVTFHGDSKHRLGELLMQCVEHNGFERLIRLLSILQEMALAQ
                     DYTLLNAGGFAVEVQAQDHERMRIICEFVDEHFHRHISLDEVAAEASMTVPAFCRYFK
                     KLTNKTFTHFVNEFRIAHACRMLSDEHLSISAVSYESGFN"
     gene            8324..>8785
                     /locus_tag="PK198_11275"
     CDS             8324..>8785
                     /locus_tag="PK198_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39364.1"
                     /translation="MNTLLKKSWPHLASMMAFLLLSIAFFQPQLKGKLVQQSDIMQYL
                     GMAKEANDYEKKNGEPALWTNSMFGGMPTYQINTVSAGNSLKVLEKAGRLFIAEPIGQ
                     FLLAMLSFYILMIVIGASPLVAAIGAVAFGFTTNNFILYEAGHLTKLKSISF"
     assembly_gap    8786..8821
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8822..>9087
                     /locus_tag="PK198_11280"
     CDS             <8822..>9087
                     /locus_tag="PK198_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39365.1"
                     /translation="AAFRGRYLLGGALFAVGLGLNIYSNHIQMTYYLALTFLFFGVAQ
                     LVYDVKDGKILHFAKAAGVLLLGAGLALGSTASNLMTTYEYSKDT"
     assembly_gap    9088..9226
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9227..>9464
                     /locus_tag="PK198_11285"
     CDS             <9227..>9464
                     /locus_tag="PK198_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39366.1"
                     /translation="GFIPGVAGGGSQEKVGKSSNFYKELEPMYRQSGRAAPPTFKIPL
                     YWGKLPFTSGPIYFGAVAVFLFLLGLLTVKGPAKW"
     assembly_gap    9465..9484
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9485..>10077
                     /locus_tag="PK198_11290"
     CDS             <9485..>10077
                     /locus_tag="PK198_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39367.1"
                     /translation="LGTLLTILLSMGNNLEAFNRFFFDHVPLYNKFRTPNSVLSITSF
                     LIPALGFMALAGVVKNEKPNATDKLGLYIALGISGAMALFFAVAGPSFFDFVNPGDER
                     FLQKTFSQESLIADRKSLMRGDAFRSLLLVLVSAGLIWTYWSGKIGRNILLGGFAVLV
                     LFDMWSAGRRYLDSEDFTDRTNYEAQFKPTPADEMIF"
     assembly_gap    10078..10106
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10107..>10455
                     /locus_tag="PK198_11295"
     CDS             <10107..>10455
                     /locus_tag="PK198_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39368.1"
                     /translation="DRSDPQSNPFASSRASYFHKSIGGYHAAKLQRYQDLIDRHLTKG
                     NISVFNMLNTKYFIMSDEDGTPSLQPNVDACGHAWFVSNINMVKDANAEIAALETFVP
                     EEDAFVHQEFESYV"
BASE COUNT         2479 a         2561 c         2671 g         2412 t
ORIGIN      
        1 ttggatgccg gcggtcaaaa cgaagccgtg cattgggggc aaatggtact gcgcagcatg
       61 gtgggacatg ccgggcagta tgtcgtgcag tcgccgcagt ctttgttcgg caccaatcag
      121 ggcgtttcca aaatcattga atctaccggc gttattttgc tggatgccat tctggacgac
      181 aacagcgacc agattgtttt caaaaatgct ctcagccccg acacgctcga ccgcctcacc
      241 cgcgcaacgc tggatgtagt ggctcagcac cccgccgtgc tgcacggagg ccgaggcatc
      301 aaagaaatca ttgcaggcgt ggcgggtgcg gtgaaagaca aaagcatttt ggaacgggtt
      361 tcctgcccga actcacccgc gttgtgctgg agcaatcggc gggacatctc gatctgttgt
      421 ggagagaaac gcccaccggg gccgagcact tgctggtatc ggcagtgaag caggtcatgg
      481 ggacgctggc tgctcaaacc accgacggaa aaccctggaa accggcgctg accaacacac
      541 agatgctctc cattgtagaa gaattggtgg atgaagtggt gcagaatccg gcctggatat
      601 tggacgaagt gcagggtaaa cccgtgcttt ctgaaatgct ggatgctacc tttgcttcca
      661 tgcgctcgct gcccaaagac gctcgtttgc agggcgaggt attgcgcagt attttgaaac
      721 agaatgtgga aatcacgctg ctgcaacgca gtgtgctcga taaaatcaag tggggaaccg
      781 agcaggaaga aaaggccatt ttgagcaagg ctttggaaat ggtgttcact tttgttttcc
      841 ccaaagatgc ccgcccggag attggtcgcc tgcaattgct ggctgaactg ttggagtacg
      901 tcacccagac tttgctgcgg cagcaccccg acgaaaaagg attggtattg ctcgatctca
      961 tcttattcaa aggcggggtt gattttagcc ggggcttcga taaaaaactg acggatgaga
     1021 ttttgaactc ggtaagcgcc gcgctggcag cacaccccga attgttggta aaaggccggc
     1081 tattgcagag catcatttcc gacctggcgc agacggtgca ggattccggc atcagacagc
     1141 ccggcctgct gcccgaactc atacggatta cgctggaaaa cgtggcctca cacttggaag
     1201 ccgtagccga cgaacatgca gggcaaccca aacacattct cgtgctggcc atcgggcagg
     1261 tgttgcgggc catttcgcaa ccttccgaag atgggcgctg ggtaccgaag ctgtcgaacg
     1321 atcagattca ggnnnnnnnn nnnnnncgtt gttttggaaa accctcagtg ggtgcgcggc
     1381 ggcgacaagc tgttccaact cattgatgct gtgatttgcg ccatggagaa actgcctgat
     1441 tttgtggctg ttacatacac cttgctccgg gatttgatag aaattgcgct cgaagcagta
     1501 gagcggcagg ggcagttgct caacgacatc aagacacaaa ccggcgaaac cattatccgg
     1561 ctgcgttttg cactcgaaga actttttgcc atcctgtccg aggctgaaac cgcggaagag
     1621 ttagcctggt acttgtcgca acgccagata ttaaatattc tgatggaata cttcctcact
     1681 tatatcgtca ccattcaggc taaggaagac actctcaaaa cagcggtaag ccagatgaaa
     1741 gcggctgtgg caacgtggcg aaacgatacg agcaagacgc tggaagaaat tttcggaaaa
     1801 ttggggcaac agtctgcttg atttttgata atccaaacaa taccaaaacc atgaaaaaaa
     1861 tcgctcgttt taccaccggc cttgcagcgc tggttttgtt gctggcactg agttcctgca
     1921 gccatttgat gtacttggac agcgctcagc aaaacttcaa ccgggcagcc gcactcgaca
     1981 acgaaatgcg cttcagtccg cgctccgaag tgccggcctc gccttctttg tattacagta
     2041 ccgcccatgc ttttgccgaa aaggcgcttc agaataaatc ggcgctcaaa aaagacgacg
     2101 ttttggccaa tgcctacagt atcaaggctt tgagcgaatg gaaactctat cgctacgatg
     2161 aagcaaaggc tacatccaga ttagcgttgc tggaatacca ggctttggaa gaagataaaa
     2221 aactcttcat gccccgcgac aaagcgctga tggaagccct tccgtcactg acagatttgg
     2281 atcaggggaa acgtttcctc tataccttct ttgccggcac catgccgggt tacgagcaag
     2341 ccaaagatca ttatttctcc aatgtttttc accccgatgc agacaaatcg cccaagttgg
     2401 aaaccgctgt ttcaaagata gacatggtac gctcccaatt ggagggcaac aacgatttgg
     2461 cgctgtattt tgtctcctcg caattggccg gtctcaaaac ctggagcgac gcgctggact
     2521 ttctgcgcca aagcgccaat aaggacaact cattgtcgga cagcgccaaa caagaagcgc
     2581 gggctttcgt agccgagcaa tacaagctgc acattaagcc acaaaaagaa aaactgctgg
     2641 ccgatctgag tcgtctggct cccaagcccg aagctgaaag gatgaagtct tactgggatg
     2701 atttgttttg agatgttgta gcccgccgca tcgtaaaagg gcactttttt cgtaaacaat
     2761 cccgcggatt cgccgtttca ttcagccaca aagcatactt ttgcaccgtt tttcttgaat
     2821 catcaaattt ttaatcatgc acgcagtagc gaaccaattg aaatacaagg taaaagacat
     2881 ctcgctggcc gactggggcc gcaaagaaat agagttggcc gaggccgaaa tgcccggtct
     2941 gatggctctg cgcgagcaat atggcgcgct gaaaccgctc aagggcgccc gcattgccgg
     3001 ctgcctccac atgaccatcc aaactgccgt gctcatcgaa accctcgtgg aattgggcgc
     3061 cgaagtgagc tggtcttcct gcaacatctt ctctacgcaa gaccacgccg ccgctgccat
     3121 tgctgccgca ggtattccgg tgtatgcctg gaagggccag aatgaagagg aattctggtg
     3181 gtgcatcgag caaactttgt acgcatttga aggcggcgaa ccgctcaata tgatccttga
     3241 cgacggcggc gacctcacca acttagtgct ggaccagcgc cccgaactca ttgctggagt
     3301 caggggcatc agcgaagaaa ccaccaccgg cgttcaccgc ctgtacgagc gcgtcaccaa
     3361 cggtacgctg cccatgccgg ccatcaacgt gaacgactcg gtgaccaaat ccaaatttga
     3421 caacaaatac ggctgcaaag aatctgccgt ggatgccatt cgccgcgcta ccgacatcat
     3481 gatggcaggc aaagtggccg tcgtggctgg ttacggcgac gtgggcaagg gcacagccgc
     3541 ttctctgcgg ggcgccggtt gccgcgtgat cgttactgaa attgacccga tctgcgcact
     3601 ccaggctgcc atggacggtt ttgaagtgaa aagaatggtc aacgccatcc cgcgcgccga
     3661 catcgtcatc actgctacgg gcaaccgcga catcgtgacg gaggagcatt tctacttgat
     3721 gaaagacaaa gctatcgttt gtaatatcgg ccacttcgac aacgaaatag atatgtcctg
     3781 gctgaaccaa agcttcggca aaacgaaagt gaccatcaag ccgcaggtgg acaaatacac
     3841 cctcaacggc aaggacatca tcgtgctggc cgaaggacgt ttggtcaacc tcggttgcgc
     3901 tacggggcac ccttcgtttg taatgtccaa ctcattcacc aatcaggtac tggcccagat
     3961 cgaattgtgg aagcactccg accgctacga aaatgcggtt tacacgctgc ccaagcattt
     4021 ggacgagcag gtagcccgcc tccacctgag caaaatcggc gtggaactcg aagaactcaa
     4081 cgaggatcag gcttcttaca tcggcgtgaa gaaagaaggg ccgttcaagc cggaatacta
     4141 ccgctattga ttggcacaag cagttgaaaa tcatacccaa acgcgtcgga gaagagttgc
     4201 ttctccggcg cgttttttat tggctcaccg ttttttgccg gctacaatct cgaaggtttg
     4261 caccaaacga tagccgtctt cgtccaccaa agtgagttgg tgggttccgg gcgcaggctg
     4321 gagcgtcata ttgtggtagt tttcggttgt gccgagaaac tcctcatcca agtgccagta
     4381 tatttttttg gaagcattgc gatgagcaac ggtaaaaaca gttcccgtaa cgctgccgtc
     4441 taaattgaca ggcacataaa tgcgggccgc ccgacggggg taaatcaact gcatgggcaa
     4501 gcgggcgtcc ttcacctcgc aatcggggcg cagtggcgga agtggctgat aatctgcatg
     4561 tttggcgatg taaaaaaact cttcgagagg aggtagcaca aaccagggct gatgcacgat
     4621 ggctgtcggc gattcacaat cggcatgaac ccgccatttt ttgccggcat ccaagtgaat
     4681 ggtttcgtga taaggacagg cgggcgtgtt gactgccgaa cgcggcgccc acacactgtc
     4741 gcgagggcat atactcaaag gctggtaacc actttgggcg cacaccggca accgaatcat
     4801 ttcatcataa ggcgggtaaa accaacgacc ggtgcgcaat aattggaaaa catcaaacag
     4861 caccggggca gccgcttgta ccccgatgag gccgggcctg ccttctccgt cggcattgcc
     4921 tacccaaacg cctactgcat aatgcggcgt gacccccaca gcccaggcat ccctgaaacc
     4981 aaaactcgtt ccggttttcc aggctacggg tctgccggag ccgaacgatt cccagtcgcc
     5041 ctcttcgctt gggcgttcca attgccgcat ggcctccaat gcgagccagg cagcccctgc
     5101 gttcaaatgg gtaggctctc tgcgaagccg cccggagcta tggttgtcgg acggtgcaag
     5161 caaccaggtg caatgcctga agtcgcttgc gttatagcgg ctgttgtact ccgggaaatg
     5221 ctccaacatt ctggccatag aggcgtaaca attggttaca tcccaaagtg ttgtttcggc
     5281 gcctcccaat atgagcgaca ggccatagtg agtaggcggc tttgtaatgg ttgaaaattg
     5341 cagcttgttg agcgcaaaat gaaatcgttc tactccgtaa tcctgcaaca agtgtacaaa
     5401 aggcacattc agcgatctgc tcagggcgcg acgggcgggt ataactccct catactgttt
     5461 gccgaagttt tcgggtttga aatcccggag ctgtaccggc acatccgaca ggaggctgcc
     5521 gggcaatatc cggccttctt gcaatgccag ggtatagagc aaaggtttga gaatactccc
     5581 tgaacttctc ggcgctgtga caatgtccac ctgctcgcca tgttcggcgc cagcgtcggg
     5641 cacattgcct aaatacgcca gtacttcccc tttggacacg tcgagtacca aaatggccat
     5701 attgtgtatg ccgttggcgg tcaatatgct atgtcggcga atggctattt cattgacctg
     5761 tttttgaagt tcggcccgga gtgtggaatg tacccttgcc gggccaggag atttaggccg
     5821 ggcgtattgg gcgcaagctc ggtccaagag gtgcggagct tcgcggggca gcggatgggg
     5881 tttgtcgggc agcggttctt ccttggctaa tctgcaagaa gtttcatcta tgatgcctct
     5941 ctggaaaagc ctgtccaaga ggcggttgcg tttttccatc agggcattcc tgtttctgcc
     6001 gggatgaatg agcgccgggc tgttgggcaa tacggccaat gtggcggctt cggcccaaga
     6061 caagagttcg gggcttttgc cgaagtatct ccaggaggcc gcatccaaac ctactacgtt
     6121 gcctccgaaa ggcgcgtgtg aggcatagag cgcgagtatg gaacgtttac ttttgccaag
     6181 ctccaaacgg gtcgccatga acgcctccgt cagtttgttg aagagacttc ggggtttgtt
     6241 ttttcgggcc atacgaatga cctgcatggt aatggtactt ccgccgctga cgatgcgacc
     6301 accggaaata ttctggcgca gggcgcgccc aaatgccaga acatccacgc cgggatgtcg
     6361 atagaaacgc ttgtcttcaa actcaatgag cgctttttcg taccgctctg aggacaaagt
     6421 gtctaaagcc gggaagcgcc attggccgtc tgctgcgatt tttgcgccga gcaattcgcc
     6481 ggagcgatct tccagcacaa cgctgacagg gtcttgaaac aagggactcg gcaaacaaaa
     6541 gagccaaagt agaaacaggc cgtacagcgg aaagagccat tttttatggc gcagggaaaa
     6601 tgtaaaaaaa cggcttgcga aaagctgaac agccttcggg aaatcggcaa ttttttgtct
     6661 gaaaaaacgg cctgaaatat tgatagccgt tttcataaca ttgtggtata agccgtatta
     6721 cttggcagga accatcaccc gactgaaaat gaacgggtcg ttggagtccg ggcgaagtac
     6781 tattttttgc ggagtcattt ccagaatatc gtaaggcgga gccgaagtct tggtaacctg
     6841 catcaaaatc tgatccccac tgattttcca atagccggta gtttcctcgc catttagatt
     6901 gataatcaga agattgnnnn nnnnnntttt taaattcaac agtggcatct ttcaggcgat
     6961 tcatcacacc ctccatgaaa ttgagttggg catcactcac aggttctttg gtcatgagtt
     7021 cacgagtggc ctcaaggtcg tatttccacg cggttgccat gacgattttg tcacgattat
     7081 tgcatgaaaa catagagaag gccaaagtca gcagcaaaag gtatcgcatc ataattctgt
     7141 ttagaagttt tttttgcagc gccaaaaata gacctgttcc ggggaatttc agcaattttc
     7201 aaaaggaaaa ctttaggcgc gcaccatttt gagcagtgat cgcctgnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn gttgaatccg ctttcgtagc tcactgcgga aatactgaga tgctcgtcgg
     7381 acaacatgcg gcaggcgtgc gcaatgcgaa actcattgac aaagtgtgtg aaagttttgt
     7441 tggtcaactt tttgaaatac ctgcaaaaag cgggcaccgt catgctcgcc tcggctgcta
     7501 cctcatccaa cgaaatgtgg cgatgaaagt gttcgtccac aaattcgcat attatccgca
     7561 tgcgctcgtg gtcttgtgcc tgtacctcta cggcaaaacc tccggcattg agcagggtgt
     7621 agtcttgtgc cagcgccatt tcctgcaaaa tggacaaaag gcgtataagt cgttcaaaac
     7681 cattatgttc tacacattgc atcaggagtt ctcccagccg gtgtttggag tctccgtgaa
     7741 aagtaacgcc ctgatgcgaa cgctcgaaca atagccggat ggcattcatt tccggtttgg
     7801 acaaaaactc cgcaccgaga aagtcttccc tcatttgcac cacaatctca acgtgctcgt
     7861 cgtacaactc ttctgaaaag ccgaaatgag gcatgttggg gcctaaaaaa atcaggtcgc
     7921 catgttcata aaaagagacg tgctcgccaa tgtgacgttt gccgcgccca ttggaaatat
     7981 aaacaatctc atactccggg tggaagtgcc agtaaggttt acccagatag ttgatgtctg
     8041 taaatttgcg aatggtaaaa gagttgccga ataccggctc tattttttct aatgtagctt
     8101 tcatggcttg ggtattgcgt atagtgtttg aaactatttt ttgttacgac gcaaaattaa
     8161 cccgaaaggt tgtaatgaaa caagtaatgg tcaatatagc attagtattg gcaaatcgaa
     8221 gcaaggtttc agcaaggccg gggaggtcgc tttcttgcat attttgtata attgaaggga
     8281 ttttcgtacc tatgccgctc aaaaccaaag ggtcaaccat agtatgaata ctttgctcaa
     8341 aaaatcgtgg ccgcacctgg cctccatgat ggcttttttg ctcctgtcta ttgccttttt
     8401 tcaacctcaa ctgaagggga aattggtgca gcagagcgat attatgcagt acctcggcat
     8461 ggcgaaagaa gccaatgatt acgagaaaaa aaatggggag ccggccctgt ggaccaattc
     8521 catgtttggc gggatgccta cctaccagat caacacggtc agtgcgggca acagcctgaa
     8581 agtgttggaa aaagcaggcc gcctgtttat tgccgagccg attggtcagt ttttactggc
     8641 gatgctcagc ttttacattt taatgatcgt cattggcgcc agccccttgg tggcggctat
     8701 cggggcggtg gctttcgggt ttaccaccaa taatttcatc ctctacgagg cggggcacct
     8761 caccaagctc aaatccattt cctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 ngccgctttt cgggggcgct acctgctggg cggggcgctc tttgcggtgg gtttgggctt
     8881 gaacatctac tccaaccaca tccagatgac gtactatctg gcgctcactt ttctgttttt
     8941 cggcgtagcg cagttggtgt atgatgtcaa ggatggcaag atattgcatt ttgccaaagc
     9001 cgccggcgta ctcttgctgg gcgccgggct ggcgctgggt tctactgcct ccaacctgat
     9061 gacgacgtac gagtattcaa aagatacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggg tttattcccg
     9241 gtgtggccgg tggcggatcg caggaaaaag tagggaagag ttcaaacttt tataaagagt
     9301 tggagccgat gtaccgccag tcaggaagag cagcgccgcc aacgttcaaa attccgctgt
     9361 attggggtaa actgcccttc accagcgggc cgatctactt tggcgcagtg gcggtgttcc
     9421 tgtttttatt aggattgctc accgtaaaag gccctgccaa atggnnnnnn nnnnnnnnnn
     9481 nnnnattggg cacgctgctc accattttac tctctatggg caataatctg gaggcgttta
     9541 atcgcttctt tttcgatcat gtacccctgt acaacaaatt tagaacgccc aattcggtac
     9601 tcagcatcac ttcattcctc attcctgcac tgggcttcat ggccttggcc ggggtggtga
     9661 aaaacgaaaa accgaacgca accgataaac tcggactgta catcgccctg ggaatcagcg
     9721 gcgctatggc gctgtttttt gccgttgcag gcccgtcttt tttcgatttt gtcaaccccg
     9781 gagatgaaag attcctccaa aagacttttt cgcaggaatc gttgattgcc gaccgtaagt
     9841 cgctgatgcg cggcgacgct ttccgctctt tgctgctcgt gttggtcagt gcgggcctga
     9901 tctggacgta ttggtcgggc aaaatcgggc gcaatatttt attaggcggt tttgctgttc
     9961 tggtgttgtt cgatatgtgg tcggctggtc gcaggtactt ggacagcgag gactttacag
    10021 accgcaccaa ttacgaagcg cagttcaagc cgactcctgc cgatgaaatg attttcannn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnatga ccgctccgac ccacagagca atccattcgc
    10141 ttcatcgagg gcttcttatt tccacaagtc catcggcggc taccatgcgg ccaagttgca
    10201 gcgctatcag gatttgatag accggcattt gacgaaaggt aatatttctg tattcaatat
    10261 gctgaatacc aagtatttca ttatgtctga tgaagatggg accccctcgc tccagcccaa
    10321 tgtggatgct tgtggccatg cctggtttgt cagcaatatc aatatggtga aagacgccaa
    10381 cgcggagatt gcagcgctgg aaacctttgt tcctgaagaa gatgctttcg tacatcagga
    10441 gtttgaatct tatgt
//
LOCUS       DAORLF010000123        10429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3420_length_10429_cov_1.941681, whole genome shotgun sequence.
ACCESSION   DAORLF010000123 DAORLF010000000
VERSION     DAORLF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3420_length_10429_cov_1.941681"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>946
                     /locus_tag="PK198_11300"
     CDS             <1..>946
                     /locus_tag="PK198_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39369.1"
                     /translation="PLQLGTNVSGPGMTYQWRGPNFMSANQSPVVSPAAGPGTGGIYT
                     LRVTQNGCTSAPDTTTVTVLPKPATPILGSSGPACEGSTILLTASTPASQYRWIPPGG
                     GTPIVTTVNVLLLQNVTSAVAGPWRVFVTQFGCDSDVSAPLEVVVNLIPQVSVEASAM
                     SVCEGQPLQLFASPILANGQYAWSGPLNFSSNQRTPLLSPMTLDMAGTYSVTVTTAAG
                     CFASASVAVQVLPRPVITGLSNDGTACIASPFDVQFMATLSPPSGNYTFEWSGPCQVM
                     AAPSGGMIPGATTSCNGAYQLIVRNEFGCASLPATTFVN"
     assembly_gap    947..987
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <988..1526
                     /locus_tag="PK198_11305"
     CDS             <988..1526
                     /locus_tag="PK198_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39370.1"
                     /translation="GNAGAVCAGQQLILTTSSYTGNSVTYYWQTPAGLVPTSTPSLTI
                     AASTLADSGPYSVFVEVDGCPSSPSGLINVVVSPVPVIVASSNSPVCAGQTIQFNSNF
                     IPAGMYQWTGLNNGFTSAISNPSISNADSLAHSGAYVVTVSRNGCISNPDTVFVLVRN
                     RPMRPVALAVAPVCISEP"
     gene            1683..3080
                     /locus_tag="PK198_11310"
     CDS             1683..3080
                     /locus_tag="PK198_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF39371.1"
                     /translation="MRARKDGCVSALSEPVFVTFNTIPSQPPFAGEDATVCPNQDIFL
                     NAAAPPRGSGVWTLTAGGTGNEVIESPAQAGSQVTGLLMGQTYTFRWTLSNGACVNYG
                     FDEMDISVRQIETAQAGVDTTICPGQPFELYATSVAIDTGRWSQLDIQALFGVMIQDS
                     SAANTAVTGMTSGNQYLFVWTVSGACGILRDSVIVSLSDVQPRAGDDFRACNDEQFAV
                     LRAQSPGQGSMGLWSSPDTTLRFSNATAPDATVFGLSEGQNMLIWSIDAGLCGAASVD
                     TLIVTWSPNPVAENDTIEVNFGEQSLLYVWANDFIPSGTTIRIISPPQFGTTTIVGDT
                     AILYTPDLNFTGADQLNYEICSEGCECAIATVFIRVSAGSLCNPPTVITPNGDKVNDQ
                     LVIPCLFDTQAFPRSQVLVFNRWGDEVFRSSIPYRNNWEGTFNGEDLPPGTYFYIINY
                     GDGTVPKTGYLMILR"
     assembly_gap    3229..3437
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3438..4065
                     /locus_tag="PK198_11315"
     CDS             <3438..4065
                     /locus_tag="PK198_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF39372.1"
                     /translation="GRGVLGLGLQASVRLLRVDYAALEGTQSIDLDGAVPASMQSKYI
                     PNFGFGAYYSTDRYYVGLSAPRLLQNNINLSEVQNLVNRERRHYFFMGGLLLPMGEKV
                     QFQPQLLLKYVNDAPFDGDVNLNFVFNDKYTTGLSYRLGGSKSNNAGEAISLLMAAQI
                     TDNLLLGLAYDATLTELRNFSSGTVELALRYSIGGRSKGREYVNPRFF"
     assembly_gap    4226..4242
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4303..6324
                     /locus_tag="PK198_11320"
     CDS             4303..6324
                     /locus_tag="PK198_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF39373.1"
                     /translation="MLRISILFLLFLAPLAGIAQRKAKKVKAAAAPALEAQTTNGIEY
                     YNVKTLNTSSLDFSPVYYQNGIVYVSSRRKSGPVDRKIGETFFELFYSELGPEGKPGK
                     PVTFSVELNSRLHEGPVSFNREGDRIFFTRNNVQDGITRADAQGRVGLKIFEAVKGIY
                     DWESITELPFNSDDYSCMHPSLSPDGQRIFFASDKAGGYGGFDLYVVERRGAMWSEMI
                     NLGPEINTAGNEVFPFIHETGSLFFASDKGMGGLDIFSVDLSSRTWGAVNNLGEPFNS
                     VSDDFGLVFHPDSKGGFFTSSRAGGLGKDDLYGFRSPNGIRGVAQPVDIPLMVTVYDG
                     EKNRRLPRAELRLFQRGSDGLMANTALYNVEMTPVQGGSAEMVLRLVRKRDDELGLPL
                     AHTNRDGEAQILLPPGKDYILAVHAPGFASKEILLLAKNPGAIQPLDILLEPESCVQL
                     SGNVQSLNYATVVPNVLLRLVHEKSGREEYIRATIEGKFDYCFDPGSDYTIYFEKEGY
                     AVERYRLTAQQIQAGNVAPLDIKLLAGSESSVREPLKEGTVILLQNMYYDFGRSAVRT
                     GESRDLDALLRVMQLFPSMHIELASHTDSRGAADYNLQLSLKRAEAAKEFLVQRGVDA
                     ARIRTAGYGESRLRNGCKDGVPCSEAEHQQNRRTEVRVIRMDEQRREGN"
     gene            complement(6404..>6839)
                     /locus_tag="PK198_11325"
     CDS             complement(6404..>6839)
                     /locus_tag="PK198_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF39374.1"
                     /translation="KKSDTNHAPPPPPPPAEPEAEEIFKVVEEMPRFPGCENLATTDE
                     RKACADKKMLQFIYDNIQYPSIARENGIEGTVVVRFVVEADGSIKNAEVVRDIGGRCG
                     EEAMRVVSLMNGLPQKWTPGRQRGKPVRVWFNLPVKFILEYN"
     assembly_gap    6840..6886
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6887..7350)
                     /locus_tag="PK198_11330"
     CDS             complement(<6887..7350)
                     /locus_tag="PK198_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF39375.1"
                     /translation="MNTVFSFTNQEFLLLAGGVIVVIAMMIYLFRRHYGTLSATGSLS
                     DKHNAARNTGAWDVQKKYPEVDVFRWSGMFFRISLVAVLAILLGAFNLTTYERSGHIP
                     KGALDLGDLDIEVEVPRSAEATPPPPPPPPPVIQEVPNETILETDDVFVDNSV"
     gene            7624..8007
                     /locus_tag="PK198_11335"
     CDS             7624..8007
                     /locus_tag="PK198_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39376.1"
                     /translation="MVAEKTDVQSLHFEHRLWDNELKFFADEMVIFEHRLEEMVKKHE
                     EPEALMRLEQFQNKFIRQKAVLQELAHDIKIHEQTISGLLKAGFGTPEHKVTDHTMLR
                     ERMESFREIYAEVKEDFYRYLLVWK"
     gene            8081..9556
                     /locus_tag="PK198_11340"
     CDS             8081..9556
                     /locus_tag="PK198_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRF39377.1"
                     /translation="MTEVALVFPHQLFRSHPALHTSRPVYLAEEWLFFRQYRFHQQKL
                     LFHRASMQYYRDFLLQSGFEVRYIEAMQPQADVRLLMQHLHEAGVQRISLCEVTDNWL
                     HQRLIRSARHFGIELCFFPSPLFILQEAEAEGYFSGKMRFFQTDFYIQQRQRLQVLLD
                     ENNKPLGGKWSFDAENRLKYPPRQTPPSPMMPAENPWRREAAEYVSAYFSANYGSVQG
                     GISYPVTYEESEAWLHDFVRTRLAKFGDYEDAMVVKEGILHHSLLTPMLNVGLLTPRQ
                     ILEAVLEAQRRDGISLNSVEGFVRQIIGWREFIRGVYEAAGTKERTTNFWGFRRKIPA
                     TFWIGNTGIAPVDIVVRRVLATGYCHHIERLMVLGNFMLLCEFDPDEVYRWFMELFID
                     AYDWVMVPNVYGMSQFADGGLMATKPYISGSNYLMKMSDFPKGDWQQVWDALFWRFMH
                     THRSFFLSNPRLGMLVKTFDKMPAEKQQAHLERAEAWLAGV"
     assembly_gap    9718..9756
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9757..>10429)
                     /locus_tag="PK198_11345"
     CDS             complement(<9757..>10429)
                     /locus_tag="PK198_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRF39378.1"
                     /translation="GDDFAFAIERKNPAGSEVLETGYFSSARESGAGNDDIFRYEKRI
                     PPPIPPKADTVQKQQAFKMLLDVFVLEKIYENPADPNSKVLGRKPLAGAQAEMRNGAE
                     TKKWTVNEEGLFTIELADNTDYSFLAAKPEYLNNAAAFTTKGIGKDTTQAEMRFELEI
                     VLDRIFIDKEIRLENIYYDFDRWDIRTDAQPTLDKLARLLLINPGIRIQLGSHTDCRG
                     NDRYNQ"
BASE COUNT         2201 a         2691 c         2825 g         2359 t
ORIGIN      
        1 ccgctgcaac tgggcaccaa tgtcagcgga ccgggcatga cctaccaatg gcgcggcccc
       61 aattttatgt cggccaatca gtcgccggtg gtatcgccgg cagccgggcc gggtacggga
      121 ggcatttata ccttgcgcgt gacacaaaat ggctgtactt ctgcgcccga cacaacgact
      181 gtgaccgtgc tgccgaagcc cgctacaccg atattgggca gcagcggacc agcttgcgaa
      241 ggcagtacca tcctattgac ggccagcacg cctgcgtctc agtatcgctg gattccgccg
      301 ggcggcggca ccccgatagt gactacggtg aatgtgcttt tgttgcaaaa cgtgacttct
      361 gccgtagcag gtccctggcg agtgtttgtt acacaattcg gctgcgattc cgacgtgtcg
      421 gcgccgcttg aggtggtggt gaatctcatt ccgcaggtat cggtagaagc ctcggccatg
      481 tctgtttgtg aagggcagcc gctgcaattg tttgcctcac ccatattggc caacgggcag
      541 tacgcctgga gcggcccgct gaattttagt tccaaccagc gcacgccgct cctatcgccg
      601 atgactttag acatggccgg cacttactcc gtgacggtga cgacggcagc ggggtgtttc
      661 gcctcggctt cggtagcggt acaggtgctt ccccgaccgg tgattacagg tttgagcaat
      721 gacggaacag cctgtattgc atcgccgttc gatgtgcagt ttatggccac cctcagcccg
      781 ccttccggca attacacctt cgagtggagc ggcccctgtc aggtaatggc ggctccgtcg
      841 ggcggcatga tacccggcgc tacgacctcc tgcaacggcg cttaccagtt gattgtaaga
      901 aatgagttcg gctgcgcttc tttgccggcc actacttttg tcaaccnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnncgg gcaacgcggg tgcagtgtgc gcaggccaac
     1021 aactgatttt gacaacatct tcctataccg gcaactcggt gacatactat tggcaaaccc
     1081 cggcaggtct cgtaccgacg agcacgccct ctctgaccat tgctgcctct acccttgccg
     1141 acagcggccc ttactccgtt tttgtggagg tggacggctg cccgtcttcc ccatcggggc
     1201 ttatcaatgt ggtagtcagt ccggtgccgg tcatcgtcgc atcgagcaac agccctgtgt
     1261 gtgccgggca aaccatacag ttcaattcca atttcattcc tgccggcatg tatcaatgga
     1321 cggggctgaa caacggcttc acttcggcca tttccaaccc atccatcagc aatgccgact
     1381 cgctggcaca ctccggcgcg tatgtggtga cggtgagcag aaacggctgt atttccaatc
     1441 ccgacacggt ttttgtgttg gtgcgcaatc gcccgatgcg gcccgtagct ttggccgtag
     1501 cgcctgtttg tatcagcgag ccgtgagcgc gggttatttt ttccgtagct ccggcatctg
     1561 ctacgcctgg cgccagttac gactggtttg accaaaataa tacgcagatt ggcgtttcgg
     1621 cgggtctcga ttttcagttc aacaactttg ccccgtatgg cgaaggcacc ttccagtttt
     1681 ttgtgcgcgc cagaaaagac ggttgtgtgt cggccttgtc ggagcctgtt tttgtaacat
     1741 ttaataccat tccttcgcag ccgccttttg ccggtgaaga cgctacggtg tgccccaatc
     1801 aggatatttt cctgaatgct gcggcgccgc ccagaggttc aggggtgtgg actttgacag
     1861 ccggcggcac gggcaatgaa gtcattgaat cgccggcgca ggcaggttcg caggtcaccg
     1921 gcctgttgat ggggcagact tacactttcc gctggacact ctccaacggc gcctgtgtga
     1981 actacggatt tgacgagatg gacatcagtg ttcgccagat agaaacggcg caggccggcg
     2041 tggacaccac gatatgcccc gggcagccct ttgagttgta cgctacatcg gtagccatag
     2101 acacaggccg gtggtcgcag ctcgatattc aggccctgtt cggcgtgatg attcaggaca
     2161 gcagcgccgc caacactgcc gtgacgggga tgacttcggg caaccaatac ctgtttgttt
     2221 ggacggtgag cggcgcctgc ggaatattgc gcgacagcgt tattgtgtct ctttccgatg
     2281 tgcaaccaag ggcgggcgat gacttcagag cctgtaatga cgagcagttt gcagtattgc
     2341 gggcacaaag ccccggccaa ggtagtatgg gcttgtggtc gtcgccggat actacgctga
     2401 gattcagcaa tgcaacggcg cccgatgcta cggtgtttgg gctttcggaa gggcaaaata
     2461 tgctgatctg gagcatagac gcgggcttgt gcggcgcggc ctcggtggac acgctcattg
     2521 tgacctggtc gcccaatccg gtggcggaaa acgataccat agaggtgaat ttcggcgagc
     2581 agtctttact ctatgtttgg gccaacgatt ttattccatc cggtacaacg atccgcatca
     2641 tcagcccgcc gcagtttggc acaacgacca ttgtgggcga tacggctatt ctatacacgc
     2701 ctgatctcaa tttcaccggg gccgatcagc tcaactatga aatatgcagc gagggctgcg
     2761 agtgcgccat cgcaacggtc ttcattcggg tgagtgcggg cagtttgtgc aatccgccca
     2821 ccgtcattac gcccaacggc gacaaggtga acgaccagct cgttataccc tgtttgtttg
     2881 atacacaggc gtttccgcgc agccaggtgc tggtattcaa tcgctggggc gatgaggtgt
     2941 tccgctcgtc cattccatac cgcaacaact gggagggcac atttaacggc gaagacctgc
     3001 cgcccggcac ttatttctac atcatcaact acggcgacgg caccgtgccc aaaactggtt
     3061 atctgatgat tttgagataa aatcaatttt agaacgaaga aaaagaaaca tacatgaaaa
     3121 cgagattcat tttcccaata ctactgttga cggcacttcc gctgttcgtt tcggcccagc
     3181 aggaagagca atacacacag ttcatgtttt acaaattagg tttcaatcnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnggg gcgcggggtg ctggggctgg gcttgcaggc ttcggtgagg
     3481 ctgctgcggg tggactatgc cgctttggag ggcacgcaat ccattgattt agatggcgcg
     3541 gtgccggctt cgatgcagag caaatacatc cccaatttcg gttttggcgc gtattacagc
     3601 acagaccgct attatgtggg gctttccgcg ccgaggttgt tgcaaaacaa catcaatttg
     3661 tcggaagtac aaaacttggt caaccgcgag cggcggcact atttctttat gggcggcctg
     3721 ttgttaccta tgggcgagaa ggtgcagttt cagccgcaat tgttgctgaa atacgtcaac
     3781 gatgcccctt ttgacggcga tgtgaacctc aattttgttt ttaatgacaa atacaccacg
     3841 gggttgtcgt atcgcctggg cggttcaaaa tcgaacaatg ccggcgaggc catatccttg
     3901 ctcatggccg ctcaaatcac ggataacctc ctgttgggat tagcctacga tgctacgctg
     3961 acagagttgc gcaatttcag cagcggcaca gtggagttgg cgctgaggta ttctatcggc
     4021 ggcagatcga aggggagaga gtatgtgaat ccgaggtttt tttaatcagt ggggcggtag
     4081 gcggtgggcg gttttcaggg gtgagtgtgt gagaagtaag tgtgtgagga gtgagcaacc
     4141 cgaaaccttg aacacggaac ccgccttcgc caaggctacg gcggatgcaa accgaaacct
     4201 gaaaccttga acccggaacc cacctnnnnn nnnnnnnnnn nnaaggctac ggcggatgca
     4261 acccggaacc ttgaacccga aacacgaaga aaaccagaga atatgttgag aatatccatc
     4321 ctgtttttgc tgtttttggc gcctttggca ggcatcgcgc agcgcaaggc caagaaagtg
     4381 aaggccgccg ccgcaccggc tcttgaagcg cagacgacga acggcattga gtattataat
     4441 gtaaaaacat tgaatacatc ttcgctggat ttctcgccgg tgtattacca aaacggcatc
     4501 gtgtatgtgt cgtcgcggcg caaaagcggg ccggtagatc gcaaaatcgg ggagaccttc
     4561 ttcgagcttt tttactccga actggggccg gagggcaaac ccggtaagcc cgtcaccttt
     4621 tcggtagaac tcaattcccg cctgcacgaa ggccccgtga gtttcaaccg cgagggcgac
     4681 cgcatctttt ttacccgcaa caacgtgcaa gacggcatca cgcgcgcaga tgcgcagggc
     4741 cgggtaggtt tgaaaatttt tgaagccgtg aaaggcatct acgattggga aagcattacc
     4801 gaactcccct tcaacagcga cgactattcc tgcatgcacc cctcgctcag ccccgacggg
     4861 cagcgcatct ttttcgcgtc cgataaagcg ggcggctacg gcggattcga cctttatgtg
     4921 gtggaacgac gcggtgcaat gtggtcggaa atgatcaatc tggggccgga gatcaatact
     4981 gccggaaatg aagtatttcc gttcatccac gaaaccggct ccctgttttt tgcctccgac
     5041 aagggaatgg gcggtttgga tattttttca gtggatttga gctcgcgcac ctggggcgcc
     5101 gtcaacaacc tcggtgagcc tttcaattca gtttccgacg atttcggcct cgtttttcat
     5161 cccgacagca aaggcggctt tttcacctcc agccgggccg gcggtttggg caaagacgac
     5221 ctctatgggt tccgctcgcc caacggcatt cgcggcgttg ctcagccggt ggacatacct
     5281 ctgatggtca ctgtttacga tggcgaaaaa aaccgcagac tccccagggc ggagctgcgt
     5341 ctgtttcaac gcggcagcga cggtctgatg gccaataccg cgctctacaa tgtagaaatg
     5401 acgccggttc agggcggctc ggcagagatg gtgttgcgcc tggtgcgcaa acgcgacgac
     5461 gaactcggcc tcccgctggc acataccaac cgcgacggcg aggcgcaaat cctgttgccg
     5521 cccggcaaag attacattct ggcagtacac gcgccaggct ttgcttccaa agaaatactg
     5581 ctgctggcca aaaatccggg cgccatccaa cctttggaca tcctactcga acccgaaagc
     5641 tgcgtacaac tcagcggcaa cgtacaaagt ctgaactacg caacagtggt gcccaacgta
     5701 ttgctgcgat tggtacacga aaaaagcggc cgcgaggagt acatccgggc caccattgag
     5761 ggcaagtttg actattgttt cgatccgggg agcgactaca ccatctattt tgaaaaagag
     5821 ggctatgccg ttgagcgcta tcggttgaca gcgcagcaaa tacaggcagg caatgtcgcg
     5881 ccattggaca tcaaacttct ggccggttcg gaatcgtcgg tgcgggagcc gctgaaagag
     5941 ggtaccgtca ttttacttca aaacatgtac tacgatttcg gcaggtcggc ggtgcgcacc
     6001 ggcgaatcgc gcgatctgga cgccctgctc cgcgtgatgc agttgtttcc cagtatgcac
     6061 attgagttgg cttcacatac cgacagccgg ggcgccgccg attacaacct gcaattgtcg
     6121 ctcaagcgcg ccgaagctgc caaggagttt ttagtgcaac gcggcgtgga tgctgcccgc
     6181 attcgcaccg ccggttatgg cgaaagccgc ctgcgcaacg gctgcaaaga cggcgtacct
     6241 tgcagcgagg ccgaacatca gcaaaaccgc cgcaccgagg tacgcgtcat ccggatggac
     6301 gagcagcgga gggagggaaa ttgagggtat ataaaaacac acaggccaca gaactctcgc
     6361 tctgcggccc gtgattaaaa ttgtattaga ggatattttc ggatcagttg tactccagaa
     6421 taaatttcac cggaaggttg aaccataccc gtaccggctt gcctctttgg cgtcccggcg
     6481 tccatttctg cggcaggccg ttcatcagag atacgacgcg catggcttct tcgccgcagc
     6541 gaccgccgat gtcgcgtact acctcggcat tcttgataga accgtcggct tctaccacaa
     6601 agcgaaccac cacggtgcct tcgatgccgt tttcacgggc gatggaggga tactgaatgt
     6661 tatcgtaaat gaactgcaac attttcttgt cggcgcaggc tttgcgctcg tcggtagtgg
     6721 caaggttctc gcagccgggg aaacgcggca tttcttctac cactttgaaa atttcttcag
     6781 cttccggttc ggctggcggc ggcggcggcg gcggcgcatg gttggtgtcg gattttttgn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg agttgtccac
     6901 aaatacatcg tcggtttcga gaatggtttc attgggtact tcctgaatca ccggcggcgg
     6961 cggcgggggg ggaggaggag tggcttctgc actgcgcggc acttccactt caatatctaa
     7021 atcgcccaga tcaagggcgc ctttggggat gtgcccgctg cgctcgtaag tagtcaggtt
     7081 gaatgcgccc agcaaaatgg ccagaaccgc taccaaagaa atgcggaaaa acatgccgct
     7141 ccagcgaaaa acgtccactt cgggatattt tttttgtaca tcccatgcgc cggtattgcg
     7201 agcagcattg tgtttgtcgg aaagactgcc ggttgcagat aatgttccgt aatgtctgcg
     7261 aaacaaataa atcatcattg ctatgacgac aatcacgccg cctgccagca gtaaaaattc
     7321 ctgattggtg aaagaaaaca cggtgttcat acctcaggtg tttatgatga ataatttcgg
     7381 cagcttgagt tacatttctt attagtgagg ctcttactgc ttttgtcgtt gtaaaaatac
     7441 ctgcattcgg aatcccgccg gaatgatgtg tgtcagcccc cggaatgact tttgtcaaca
     7501 ggctatttcc ctgacataag tcaccgtctc ttgttgacaa gtctcatcta aaaagggcga
     7561 aaaccggggc acttttgttt ccgaacattc actacatttt tcacaccatt aaataataat
     7621 gccatggttg cagaaaaaac agatgtacaa agcctccact ttgaacatcg cctctgggac
     7681 aacgaactca aattcttcgc cgatgagatg gtcatttttg aacaccgcct cgaagaaatg
     7741 gtcaaaaaac acgaagaacc cgaagcgctg atgcggctcg aacagttcca gaataaattc
     7801 atccgccaaa aagctgtatt gcaagaactg gcccacgata ttaaaataca cgagcaaacg
     7861 atctccggcc tcctcaaagc cggtttcggc acgcccgaac acaaagtgac ggatcatacc
     7921 atgctgcggg aacgcatgga gagcttccgc gaaatttatg ccgaagtgaa agaggatttc
     7981 taccggtatc ttttggtttg gaaatagccg gcagtgagca agttcggctt ttttttgttg
     8041 tacggccttc atcctttgat tctttgattc gtacaaccac atgaccgaag tcgcgcttgt
     8101 ctttccgcac cagcttttca ggagccaccc ggcgctccac acatcccggc ccgtttattt
     8161 ggcagaggag tggctgtttt tccggcagta ccggtttcat caacagaaac tcctcttcca
     8221 ccgggcttcg atgcagtatt accgggactt tttactgcaa tcgggcttcg aggttcgcta
     8281 catagaagcc atgcagcctc aggccgatgt gcgcctgctg atgcagcacc tgcacgaagc
     8341 gggggtacaa cgcatcagcc tgtgtgaggt gacggacaac tggctgcacc aaagactgat
     8401 ccgcagtgcc cggcatttcg gcatcgaact ctgctttttt ccttccccgc tgtttattct
     8461 tcaggaagca gaggcggagg gctatttcag tggcaaaatg cgtttttttc agactgactt
     8521 ctacattcaa cagcggcagc gcctgcaagt gctgttggat gaaaacaaca aacctctggg
     8581 cggcaaatgg tcgttcgatg ccgaaaatcg cctcaaatac ccgccccggc aaacgccgcc
     8641 ttccccgatg atgcccgccg aaaatccctg gcgcagggaa gctgccgagt atgtaagcgc
     8701 ttatttttct gccaattacg gcagtgtgca aggagggatt tcctatccgg tgacatacga
     8761 ggaaagcgag gcctggcttc atgactttgt gcgcacgcgt ttagctaagt tcggcgatta
     8821 cgaagacgct atggtcgtga aagagggcat tttgcaccac agcctgctca cgccgatgct
     8881 caacgtgggc ctactcacgc cccggcagat attggaggct gtattggagg cgcaacgccg
     8941 cgatggcatt tcactcaact ccgtggaagg tttcgtgcgg caaatcatcg gttggcgcga
     9001 gttcatccgg ggggtgtatg aggccgccgg cacaaaggaa cgcactacta atttctgggg
     9061 ctttcgccga aaaatacccg ccacattctg gatcggcaac acgggcattg cacccgtgga
     9121 cattgtggta cgccgggttt tggccaccgg ctattgccat catattgagc gacttatggt
     9181 gctgggcaat ttcatgctgc tgtgcgagtt tgatcccgac gaggtatatc gctggtttat
     9241 ggaattgttt atagatgcct acgactgggt catggtgccc aatgtatatg gcatgagcca
     9301 gtttgccgac ggcgggctga tggccaccaa accctacatc agcggcagca attacctgat
     9361 gaaaatgagc gatttcccca aaggcgactg gcagcaggtg tgggacgcgc tgttctggcg
     9421 gtttatgcac acgcaccgca gctttttcct cagcaatccg cgcctcggca tgttggtgaa
     9481 aaccttcgat aaaatgcctg ccgaaaaaca gcaggcgcac ttggagcggg cagaggcgtg
     9541 gctggcgggg gtatgaaatc ctccactcac tccaaaatct taaacgtcgt gcggcggttg
     9601 cgctgatgct cctcctcggt gcagcgggtg cagaggcagg aagcttcggg agcgctttcg
     9661 ccgaaaccct gcgcgctgag gcggtcgggt tgtatgcctt tggcgatgag gtagtccnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctga ttgtagcggt cgttgccccg
     9781 gcaatcggtg tgcgagccga gttgaatgcg gatgccggga ttgatgagca gcaggcgggc
     9841 caatttgtcc aaagtaggtt gcgcgtcggt gcggatgtcc cagcggtcga agtcgtaata
     9901 aatgttctcc aacctgattt ctttgtcaat aaaaatgcgg tcgagcacga tttccaattc
     9961 aaagcgcatt tcggcctgcg tagtgtcttt tccgatgcct ttggtggtaa aagcggcggc
    10021 gttgttgagg tattccggtt tggcggcgag gaaagagtag tcggtattgt cggccaattc
    10081 aatggtaaaa aggccctctt cgttcaccgt ccattttttg gtttctgcgc cgttgcgcat
    10141 ttccgcctgc gcgcccgcga gtggttttcg gcccagcact ttgctgttgg ggtcggcggg
    10201 gttttcgtaa attttttcca gcacaaacac gtccagcagc atcttgaaag cctgctgttt
    10261 ctgcaccgtg tcggctttgg gcggaatggg cggcggtatg cgtttttcgt atctgaaaat
    10321 atcgtcgttg cctgcgcccg attcgcgggc cgaggagaag tagccggttt cgagcacttc
    10381 ggagccggcg ggattttttc gctcgatggc aaaagcaaaa tcgtcgcct
//
LOCUS       DAORLF010000124        10411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3427_length_10411_cov_1.864620, whole genome shotgun sequence.
ACCESSION   DAORLF010000124 DAORLF010000000
VERSION     DAORLF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3427_length_10411_cov_1.864620"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>440)
                     /locus_tag="PK198_11350"
     CDS             complement(<1..>440)
                     /locus_tag="PK198_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRF39379.1"
                     /translation="IGVFLLWFGWFGFNGGSVLSADPMAVSLVFVTTSLAAAAGAVGA
                     YIVSGLMFKNHDLSMVLNGILGGLVGITAGADLMTPLSAIAIGFIAGCIIPASVVFFD
                     KIKIDDPVGATSVHLVCGIWGTLAVGIFGSKAGLGQLWVQFYGV"
     assembly_gap    441..502
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<503..1083)
                     /locus_tag="PK198_11355"
     CDS             complement(<503..1083)
                     /locus_tag="PK198_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRF39380.1"
                     /translation="MQSLFTANNLWMLVATCLVFIMHLGFSTLEAGFVQRKNVVNILF
                     KNVMILAIGLLSYCVIGFNLMYPGADYAGGFFGFAGFGLSAPEGEAAAIEYASGKYTY
                     WTDFIFQAMFAATAATIVSGAVAERIKLNSFLIFSILLVSFVYPITGMWKWGGGWLNE
                     AGFYDFAGSTLVHAMGGFAALAAVLVVGPRRGK"
     gene            complement(1232..>1574)
                     /locus_tag="PK198_11360"
     CDS             complement(1232..>1574)
                     /locus_tag="PK198_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRF39381.1"
                     /translation="TMKKIEAIVRLSRFDRIRDALAQIDVNFFTLSEVKGFGLQKGEK
                     LMYRGSVYDEDFIARLKIDIFCTEEKVQPIIDAILSAGKTGEIGDGKIVVIDVENIIR
                     IRNGEAGAEAL"
     assembly_gap    1575..1619
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1776..3281
                     /locus_tag="PK198_11365"
     CDS             1776..3281
                     /locus_tag="PK198_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39382.1"
                     /translation="MPKQRDLVFKLIKSLTKAEKRHFRLFVARTKSGEEALFLQLFDL
                     LDRAREYDEKAIRTALPAGMTPTQLANLKRHLYQQIMVSLRLISTSKHIDLEIREQID
                     FARILYSKGHYMESLHTLERIKNVAEAHHQDVLHLEILEFQKLIEARHITRSRQVKNK
                     MDRLLQESTQRSLITNLAGEKANLNIQIHGYYIDHGFAVSELEKQQLQTAWRQMLPEP
                     STRRFMDTFFEKVNTYQANMWYRYILLNWAKARDHAHEWVTLFDLYPAMKFKDPDLYM
                     RSLYYLLTMLFLTGDADGYQLYFNQLESYLQEHSPAFHPNSQYVASVYHSLCRLNRYI
                     LLEDWQGGYREGLAVQQELKDYENLIDPHRVMLVEYKLAAFAFAQRDYRPALAHLEPL
                     LHHQDRILQYSMDLNARFMELMCLYSLKEWDFMEARLNSLARSMGKHKHISELQKKLY
                     ALLRELHRSPLRPPAQVAAPLLTNFERTEMTALDRYFLKYLHVEVWLRSLI"
     gene            complement(3274..>3690)
                     /locus_tag="PK198_11370"
     CDS             complement(3274..>3690)
                     /locus_tag="PK198_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39383.1"
                     /translation="HIAEMLSFPLPFRGFRYSDEEKAALVAFMHTLTDTVLLTHPKWS
                     DPFVPVTNTAHHDPLDFHWKVFPNPAAHEVFVSWPVAVSSDVEVYLKNLNGQIMRQLH
                     ATGGSYRIPLTGLPAGVYALSLQSGKRQSVKLVVIK"
     assembly_gap    3691..3709
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3710..4010)
                     /locus_tag="PK198_11375"
     CDS             complement(<3710..4010)
                     /locus_tag="PK198_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF39384.1"
                     /translation="MQENGNLNVFTPAEQQGWLLFQQTCVLCHIEGHFGTDSLFNIGL
                     EMEYPDPGLAGWTGNPAHHGAFKSPTLRNIAQAAPYMHDGRFATLEDVVEFYSNEI"
     gene            complement(4015..4686)
                     /locus_tag="PK198_11380"
     CDS             complement(4015..4686)
                     /locus_tag="PK198_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF39385.1"
                     /translation="MSRLKNRNKYLVFGLLVVAAVFIAAGNHSLPAARLSAPLVLDSI
                     APQLPDAPFDYTDIFIPGYIQQSTDSFNTPFLPLLDSNAGAALGRVLFYEKRLSANRQ
                     ISCGTCHEQALSFGAGTAGSPGFAGVVTPRNTMHINQLVFSPGTPIFWDGRVQTIQQQ
                     VIMPILHPDEMGLTEPEIIARVQEASYYPPLFEAAFGDSEVTVAGSFGLGAVCGVYGV
                     FSKQI"
     gene            4833..5507
                     /locus_tag="PK198_11385"
     CDS             4833..5507
                     /locus_tag="PK198_11385"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRF39386.1"
                     /translation="MQSYLEHVKRIFLEHGDPDISEGQMKYMRHQFAFYGLKAPVWMA
                     LAKDIFQTKGIPQGEELKTLVRLCFEEEYRELHYFATEMTQRALKKQAENFIDFLEEL
                     ITSKSWWDTVDWQAKLAGEHFLRYPQLIGPITERWMQSGNFWLQRCALIFQLRYKTRT
                     DAELMFGYVLRLADSKEFFIQKGAGWALREYSKTNPEAVRSFVESHSLAPLTRREALK
                     WLQNKS"
     gene            complement(5519..5905)
                     /locus_tag="PK198_11390"
     CDS             complement(5519..5905)
                     /locus_tag="PK198_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39387.1"
                     /translation="MRKLITLGMALATTLLLYGQSAALEVEGSPQICAGYTAASQIKV
                     RIKSSKVSTAPVSRYTLAYVWTAEHANGTRVWFSNKPYRWVPITWPGQYRVSVTLEYI
                     LPGRTRPFMVSPSSGVFLYGLDCNTP"
     gene            6104..>7143
                     /gene="glgP"
                     /locus_tag="PK198_11395"
     CDS             6104..>7143
                     /gene="glgP"
                     /locus_tag="PK198_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRF39388.1"
                     /translation="MSHVMTPVNNDMRLKRVFIEPRLPEALSPLRELAGNLWWSWNSE
                     ASALFKQIAGDQWEASQYNPVAILDEMSTEYAQTLTSDKDFMKRMKSAHSDFKNYMAA
                     KPAAAHPHIAYFCMEYGLHISMKLYSGGLGVLAGDYLKEASDSNVHLTAVGLMYRHGY
                     FQQEISMHGDQISHYPAQLFTKLPIQAVRNDKGEWVKIALEYPGRLVFAKLWLLEVGR
                     IKLYLLDADIDENSREDRQLTDALYGGDNEFRLRQEILLGMGGFKALQTLGIAPDVYH
                     LNEGHAAFVQLQRLSDLMSSKRVSLDEATEVVRGSSLFTTHTPVPAGHDNFPEDMMRP
                     YFEGFAQRLGLS"
     assembly_gap    7144..7205
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7206..>8151
                     /gene="glgP"
                     /locus_tag="PK198_11400"
     CDS             <7206..>8151
                     /gene="glgP"
                     /locus_tag="PK198_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRF39389.1"
                     /translation="ENFSMSHLAIRFCQEVNGVSRLHGDVSRRMFVDLFPGYHHEEIH
                     IGYVTNSVHYPTWIAPEWRAIHEKAFGSKFLSQQSDPAVWRRISGVSDEEIMNTRIEL
                     KKKLLRYVKSKLQTDLTRRGESPRFIFEALSNIRSSALVIGFARRFATYKRAHLLFTN
                     LERLINILQNQDRPVILLYAGKAHPADKGGQELIRHIVAISRRPDLAGKIVFLEDYNM
                     EMAKLLVQGVDIWLNTPMRPKEASGTSGMKAVMNGVLNFSVLDGWWAEGYVPGAGWAL
                     PLEATYQDQNLQNELDAETIYNLLEEEIVPAYFDRDEQG"
     assembly_gap    8152..8170
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8171..8704
                     /locus_tag="PK198_11405"
     CDS             <8171..8704
                     /locus_tag="PK198_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HRF39390.1"
                     /translation="PRQWVQHIRKTFSEVAPHFTMKRMLDDYYERFYNKLYARTHQMQ
                     LSNLADATRLTEWKHKVEHLWNGIEVRRSEIYDTANFALPMGSKFTAAIWLKLNGLQP
                     DELGLELVFFKRFGDNELNIKLRTELVFKKMEDGAALYECAVEPGMAGVYEYGVRMFP
                     RHELLPHRQDFALAKWV"
     gene            8719..9987
                     /locus_tag="PK198_11410"
     CDS             8719..9987
                     /locus_tag="PK198_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF39391.1"
                     /translation="MERYGQVLLIAIPFFLGLILIEKVYGLLKGEDKTPLMDMFSSLL
                     SGLTNTVKDVLGLSVSVLSYSWMVEHWALLHVESNWAVYVLVFVVLDFQGYWVHRWAH
                     EINFFWNKHLVHHSSEEFNLPCALRQSISSFVNLFTFFLLPAALLGVPDIVVATIAPL
                     HLFAQFWYHTRHIGKMGFWEHIIVTPSHHRVHHAINPEYLDKNYSQIFIIWDKLFGTF
                     QEELPDKPPVYGITRPVSTWNPVKINFQHLWLLAKDAWYAGSWRDKFRVWWMPTGWRP
                     ADVAARFPVAKIDDVYHFEKYNPESSAALKTWSLVHLGLLMLLSAYFFGHLGDIGSPG
                     IFVYGLFLMLSIYAMAELADRHPLAWVWELLKTGLGFFIIFRQGDWFGINSLWASGTY
                     FVAGYLIFSALMAVVLNRWGKVETATPTAV"
     gene            10045..>10411
                     /locus_tag="PK198_11415"
     CDS             10045..>10411
                     /locus_tag="PK198_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HRF39392.1"
                     /translation="MRKWYLLFVSLLAVISLHAADGHRIKVKVTGYDQKELYLAYYYG
                     DKQYVLDTATLANDGFFHFADEKPLNAGVYLVVLAPDNSYFQILVEPKEQFFSVFTQK
                     DNPSSNVKFEKSPANTQFYS"
BASE COUNT         2537 a         2714 c         2622 g         2331 t
ORIGIN      
        1 acacgccgta aaactgcacc cagagttggc ccagaccggc tttgctgccg aaaataccca
       61 cagccagcgt gccccagatg ccgcagacaa ggtgtacgga agtagcgcct accgggtcgt
      121 caattttgat tttatcaaag aaaaccacag atgcgggaat gatgcagccg gcaataaaac
      181 cgatggcaat ggccgagagc ggagtcatca ggtcggcgcc ggcggtgatg cctaccaagc
      241 cgcccagaat gccgttgagc accatagaga ggtcgtggtt tttgaacatc aggcccgaca
      301 caatgtaagc ccccactgcg cccgccgctg ccgccaatga agtggtcaca aagaccaatg
      361 aaaccgccat aggatcggcg ctgagcacag aacctccgtt gaagccgaac cagccgaacc
      421 acagcaaaaa caccccgatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnaacttgcc ccggcgcggt cccaccacca acactgccgc
      541 cagtgccgcg aaaccgccca tagcatgtac cagcgtagag ccggcaaaat cgtagaatcc
      601 ggcttcattg agccagccgc cgccccattt ccacatgccg gtaatgggat atacaaacga
      661 caccaacagg atggaaaaaa tgaggaatga attgagtttg atgcgctcgg ccactgctcc
      721 cgaaacaatg gtagctgccg tagcggcaaa catggcctga aaaatgaagt cggtccaata
      781 agtgtatttg cccgacgcat actcaatggc cgccgcctcg ccctccggag cagacagccc
      841 gaagcccgca aagccgaaaa atccgccggc ataatcagcg ccgggataca tgagattgaa
      901 gccgatgaca caataagaca gcagcccaat ggcgaggatc atcacgtttt tgaagaggat
      961 gttgactacg tttttgcgct gcacaaaacc agcctccaaa gtggagaatc ccaagtgcat
     1021 aatgaaaacc agacaggtgg ccaccaacat ccataagttg ttggccgtga aaagtgattg
     1081 catagcttgc ccttccatca caatagcttg tttaggttac agaatgaaaa ttagcgatga
     1141 ggcgaccgcg cgcaccggcg ggctgccctg ttgatgattt ggttgtgaaa aggttaggga
     1201 tgcacgttgt gaatcgtgcg ggaaaagaat gttataaggc ctccgcgccg gcctcgccgt
     1261 tgcggatgcg gatgatgttt tctacgtcta tgacaacgat tttgccgtcg cctatttcgc
     1321 cggttttgcc cgcgctgagg atggcgtcaa tgatgggttg cactttttct tcggtgcaaa
     1381 aaatgtctat tttgagacgt gcgatgaaat cttcatcata cacgctgccc cggtacatga
     1441 gtttttcgcc tttttgcagg ccgaatcctt tgacctcgct gagggtgaaa aaattcacgt
     1501 ctatttgcgc cagggcatcc cggatgcggt cgaaacgcga taagcgaaca atggcttcta
     1561 tttttttcat tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1621 ttgcctgcgc aggtcgcttg tactttgatg gcgacaaagg tagaggcgtc gggaaattct
     1681 tgaaaagcaa tgtttttgta catttgtgac gccgaaagcc agataaatcc aaacaagcaa
     1741 ctgattttca attgtatgga tttgaataag cacatatgcc caaacaaaga gacttagttt
     1801 ttaaattaat aaaatcgctc acaaaagccg aaaaaaggca tttcaggctc tttgtggcgc
     1861 gcaccaaaag cggcgaagaa gcgctctttt tgcaattgtt cgacctgctc gaccgcgctc
     1921 gcgaatacga cgaaaaagcc atccgaacag ccctgcctgc cggaatgacg cccacgcaat
     1981 tggccaacct gaaacggcat ttgtaccaac agatcatggt gagcctgcgc ctcatttcca
     2041 cctccaaaca catagacctc gaaatcaggg agcaaattga ttttgcgcgg attctgtaca
     2101 gcaagggcca ctatatggaa agcctgcaca cgctggaacg catcaaaaac gtggccgagg
     2161 cccaccatca ggatgtgttg catttggaaa ttctggagtt tcaaaaactg atagaagcgc
     2221 ggcacatcac ccgttcccgg caagtgaaaa acaaaatgga ccggctcttg caggaatcca
     2281 cgcagcgcag cctcatcacc aacttagccg gtgaaaaagc caacctcaat attcagattc
     2341 acggctacta catagatcac gggtttgccg tttccgaact cgaaaagcag caactacaga
     2401 cagcctggcg gcagatgttg cccgaaccca gcactcgccg attcatggac actttttttg
     2461 aaaaagtgaa cacctatcag gccaatatgt ggtatcgcta catcctcctc aattgggcga
     2521 aagcccgcga ccacgcccac gaatgggtca ctttgttcga tctgtatccg gccatgaaat
     2581 tcaaagaccc cgacctgtac atgcgcagcc tgtactacct gctcaccatg cttttcctca
     2641 ccggagatgc agacggatac caactgtatt tcaatcagtt agaatcatat ttgcaggaac
     2701 attcgcccgc ctttcacccc aacagccaat atgtggcctc ggtgtatcac agcttatgcc
     2761 ggctcaaccg ctacatttta ctcgaagact ggcagggcgg ctaccgggaa ggattggccg
     2821 tgcaacagga actgaaggat tatgaaaatc tcatagaccc gcaccgggtc atgctggtgg
     2881 agtataaatt ggccgctttc gcatttgcac agcgcgatta ccgcccggca ctggcacatc
     2941 tggaaccgct gctgcaccat caggatcgca ttttgcaata ttcaatggac ctgaatgcgc
     3001 gcttcatgga actgatgtgt ttgtactcgc tcaaagaatg ggattttatg gaggcgcgtt
     3061 tgaactcgct ggcacgcagc atgggtaaac acaagcatat cagcgaatta caaaaaaaac
     3121 tgtatgcact gctgcgcgaa ttgcaccgca gccccctgag acctcctgcg caagtagcgg
     3181 cgcctttact gacgaatttt gaacgcacag aaatgacggc tttggaccgc tattttctga
     3241 aatacctgca tgtagaagta tggctcagga gcctcatttg atcactacca gcttcaccga
     3301 ttgcctcttt ccggattgca aactcagcgc atacaccccg gccggcagcc ccgtcaacgg
     3361 gatgcgatac gaaccaccag tagcgtgcaa ctgtctcatt atctgtccat tcagattttt
     3421 taaataaact tccacgtcgg aggaaaccgc taccggccag gaaacgaaca cctcatgcgc
     3481 cgccggatta gggaagactt tccaatgaaa atcgagcggg tcgtggtggg ccgtattggt
     3541 cacgggcaca aagggatcag accatttggg atgcgtgagc aacacggtgt cggtgagtgt
     3601 gtgcataaag gctaccagag ccgctttttc ttcatcggaa tagcgaaagc cgcggaaagg
     3661 caatggaaag ctcagcattt ctgcgatgtg nnnnnnnnnn nnnnnnnnnc gatttcattg
     3721 ctgtaaaatt cgaccacgtc ctccagcgta gcgaagcgcc cgtcgtgcat gtagggagcg
     3781 gcctgagcga tgttgcgcag agtaggactt ttgaacgccc cgtgatgtgc cggattgccc
     3841 gtccagcctg ccaggccggg atcgggatac tccatttcca agccgatgtt gaacaaagaa
     3901 tcggtgccga aatgcccctc aatatggcac aaaacacagg tttgctgaaa cagcagccag
     3961 ccctgttgtt ccgccggcgt gaatacgttg aggttgccgt tttcctgcat ctcatcaaat
     4021 ttgctttgaa aagacaccat agacgccaca aactgcacca aggccgaacg aacccgctac
     4081 cgtcacttca gaatcgccga aagccgcttc aaacaagggc gggtaatagg acgcctcctg
     4141 cacgcgggcg atgatttccg gctcggttaa tcccatttca tcagggtgta agatgggcat
     4201 aatcacttgc tgttgaatag tttgcactct tccatcccag aaaatgggcg taccgggcga
     4261 gaacacaagt tggttgatgt gcatggtgtt gcgcggcgtg accaccccgg caaaacccgg
     4321 acttcccgcc gtacccgctc cgaacgacag cgcctgctca tggcaagtgc cgcaggaaat
     4381 ttgccgattg gccgacagcc gtttttcgta aaacagcacc cgccccagcg ccgcgccggc
     4441 attggagtcg agcaagggca ggaaaggcgt attgaaggag tcggtagatt gctgtatgta
     4501 gccgggaatg aaaatgtcgg tgtaatcgaa aggagcatcg ggcaattgcg gggcaatcga
     4561 gtccaacaca agcggagcgc tcaaccgggc ggcgggcagc gaatggttgc cggctgcgat
     4621 aaaaacggcg gccacgacca gcaggccaaa gacgagatac ttgtttctgt ttttcaaacg
     4681 gctcattccg gtgcattttg gttgacagat gagcaaagat aagtatttat cacatctgcg
     4741 gtttcttccc tatatttgct tcggttctga aacggctcat cgcccgccat gagcttcatt
     4801 ttttcaacag ataatcaaca cattacgcca ccatgcaatc atacctcgaa cacgtcaaac
     4861 gcatttttct ggaacacggc gatcccgata tttccgaggg gcagatgaaa tacatgcgcc
     4921 accaatttgc tttttacggc ctcaaagcgc cggtgtggat ggctttggcc aaagatattt
     4981 tccagacaaa aggcatcccg caaggtgaag aactcaaaac cctcgttcgg ctctgcttcg
     5041 aggaggaata ccgcgaactg cactattttg ctaccgaaat gacgcagcgg gccttgaaaa
     5101 aacaagctga aaactttatt gattttttgg aagaactcat cacttccaaa tcctggtggg
     5161 atacggtgga ctggcaggcc aagctggccg gggaacactt cctgcgctac ccgcaactca
     5221 tcggcccgat aaccgagcgc tggatgcagt cgggcaattt ctggctgcaa cgctgcgccc
     5281 tcatttttca gttgaggtac aaaacccgca ccgatgctga gctgatgttc ggctacgtcc
     5341 tgcgattagc cgattccaaa gagtttttta ttcaaaaagg cgccggctgg gcattgcggg
     5401 aatattccaa aaccaaccct gaagcggtgc ggtctttcgt ggaatcacac agtttggctc
     5461 cattgacgcg ccgggaagcg ctcaagtggc tccaaaacaa atcctgacgc ctttacaact
     5521 atggtgtgtt acaatccaag ccgtagagaa acactccgct ggaaggcgat accataaacg
     5581 gcctggtgcg gcccggcaaa atgtactcca gcgtcaccga aacgcgatat tggcccggcc
     5641 aggtaatagg cacccaacga tagggcttgt tggagaacca aaccctcgtg ccgttggcgt
     5701 gttcggcagt ccagacataa gcaagtgtgt agcggctgac aggggcagta gaaactttgg
     5761 aagacttgat gcgcactttg atctggctgg cagcagtgta acccgcacaa atctgaggag
     5821 agccttccac ttcgagagct gcggactggc catacagcaa caaggtggtt gcgagcgcca
     5881 tgcccaatgt gataagtttt ctcattttgt tatgttttgg gaagttcaaa aaaactgatt
     5941 ttcacccttt cttttcagga agcataatag cctgtttagg gcaaaccaaa gctgcccaaa
     6001 atcatattcc gcaggtgact tttgtcattt cttacactta gggggctgaa cattggctga
     6061 gaatcctaat tttgccgcct caaattcatc aacaactgct attatgagcc acgttatgac
     6121 acccgtaaac aacgatatgc gcctcaagcg cgtcttcata gaaccccgcc tcccagaagc
     6181 tttatccccg ctgcgggaat tggccggcaa cctctggtgg agctggaaca gcgaggcatc
     6241 cgctcttttt aaacaaattg ccggcgacca atgggaagcg tctcagtaca atcccgttgc
     6301 catcctcgac gaaatgagca ccgagtatgc ccagaccctc acttccgata aagactttat
     6361 gaagcgaatg aagtctgctc attcagattt caaaaattac atggccgcaa aaccggctgc
     6421 ggcgcatccg cacatcgcct acttttgtat ggagtacggc ctgcatattt ccatgaaact
     6481 ctattcgggc ggtctgggcg ttttggccgg cgactacctc aaagaagcct ccgactccaa
     6541 cgtacacctc acggcggtgg gcctcatgta tcgccacggt tattttcagc aggaaatctc
     6601 gatgcacggc gatcagatca gccattaccc cgctcagttg tttaccaaac tgcccattca
     6661 ggcggtgcgc aacgacaagg gcgagtgggt gaaaatagcg ctggaatacc ccggcagatt
     6721 ggtgtttgcc aaactctggc tcctcgaagt ggggcgcatc aagctctacc tgctcgatgc
     6781 cgatattgac gaaaacagcc gcgaagaccg ccaactgaca gatgcgctct atggcggcga
     6841 caacgagttc aggctccggc aggaaattct gctgggcatg ggcggcttca aggccctgca
     6901 aacactgggc attgcacccg atgtgtatca cctcaatgaa ggccatgccg cctttgtgca
     6961 attgcaacga ctgagcgatc tgatgtcgtc caaacgcgtc tcgcttgatg aagccaccga
     7021 ggtcgtgcgc ggttcttctc tgttcaccac gcatacacct gtgcctgcgg gccacgacaa
     7081 cttcccggaa gacatgatgc gcccgtattt tgagggtttt gcgcaacggc tgggcttgtc
     7141 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnacgag aatttttcca tgagccactt ggccattcgc ttctgccagg aggtgaacgg
     7261 cgtgagtcgc ctgcatggcg atgtgtcccg ccgcatgttt gtggatttgt tccccggcta
     7321 tcaccacgag gaaattcaca ttggctatgt gaccaacagc gtgcattatc ccacctggat
     7381 agcgccggag tggagagcca ttcacgaaaa ggcttttggc tcgaaatttt tgtctcaaca
     7441 atccgacccg gccgtatggc gtcgcatatc cggcgtttcc gacgaggaga tcatgaacac
     7501 gcgtatagag ttgaaaaaga agctcttgcg ttatgtgaaa tccaagttac aaaccgatct
     7561 cacccgtcgc ggcgagagtc cccggttcat ctttgaggct ttgagcaaca tccgttcttc
     7621 cgcattggtc atcggttttg cgcgccgttt tgccacctac aaacgcgccc atttgctgtt
     7681 taccaatctg gagcgcctca tcaacatcct ccaaaatcag gaccgaccgg tcatcctgct
     7741 gtatgcgggc aaggcacacc ctgccgacaa aggcgggcag gaactgattc gccacatcgt
     7801 tgccatttcg cgccgccccg atctggccgg caaaatcgtc tttctcgaag attacaacat
     7861 ggaaatggcc aagctattgg tacaaggtgt tgacatctgg ctcaatacgc ccatgcgccc
     7921 caaagaggca tcgggcacca gcggcatgaa agccgtgatg aacggagtac tcaatttcag
     7981 cgtacttgac ggctggtggg ccgaggggta tgtgccgggc gcgggttggg cattgccttt
     8041 ggaggccact tatcaggatc agaatttgca aaatgagttg gacgccgaaa cgatttacaa
     8101 cctgttggaa gaagaaatcg tgccggctta tttcgaccgc gacgagcagg gnnnnnnnnn
     8161 nnnnnnnnnn cctcgtcaat gggtgcagca catccgcaaa accttctcgg aagtagcgcc
     8221 gcatttcacc atgaagcgca tgctggacga ctactacgag cgtttttaca acaaactcta
     8281 cgccagaacc catcaaatgc agctttccaa tctggccgac gccacccgac tcaccgaatg
     8341 gaaacacaag gtggagcacc tctggaacgg catcgaagtg cggcgctctg agatttacga
     8401 caccgccaac tttgccctgc cgatgggcag caaatttacg gcggcgatct ggttgaagct
     8461 caacggcctg caacccgacg aactggggct ggagttggtg ttttttaagc gtttcggcga
     8521 caatgagttg aacatcaaat tgcgtaccga attggtattc aaaaagatgg aggacggcgc
     8581 cgctctgtat gaatgtgccg tagagccggg tatggccgga gtgtatgagt atggggtgcg
     8641 catgttcccc cgccatgaac tgctcccgca ccggcaggat ttcgcattgg ctaagtgggt
     8701 gtaaaaaaag caatcaacat ggaacgatac ggacaagtac tgctcattgc catccccttc
     8761 tttttggggt tgatccttat agaaaaggtg tacggcttgc tgaaaggcga agacaaaact
     8821 ccgctgatgg acatgttttc gagcctgctg tcggggttga ccaataccgt gaaagacgtg
     8881 ctggggctga gcgtgtcggt gttgtcttat agttggatgg tagagcattg ggcgctgctt
     8941 catgtagaga gcaactgggc cgtgtatgta ctggtgttcg tagtgcttga ttttcagggg
     9001 tattgggtgc atcgctgggc acacgaaatc aattttttct ggaacaaaca cttggtgcat
     9061 cacagcagcg aggagttcaa cctgccctgc gccttgcggc aaagcatttc ctcgtttgtc
     9121 aatttgttca cgtttttcct gttgccggcg gccttactgg gcgtgcccga cattgtcgtt
     9181 gccaccatag cgccgctgca tttgtttgcg cagttttggt accatacccg gcatattggg
     9241 aagatgggat tttgggaaca catcatcgtg acgccctcgc accaccgcgt gcatcatgcc
     9301 atcaatcccg aatatttgga caaaaactac agccagatat tcatcatctg ggacaagctg
     9361 ttcggcactt ttcaggaaga actgcccgac aagccgccgg tgtatggcat cacccgcccg
     9421 gtgagcacct ggaatccggt caaaatcaat tttcaacacc tgtggctgct ggccaaagac
     9481 gcctggtatg ccggcagttg gcgcgacaaa ttccgcgtgt ggtggatgcc caccggttgg
     9541 cgcccagccg atgtggccgc tcgttttccg gtagcgaaaa ttgacgacgt gtatcatttt
     9601 gaaaaataca atcctgaaag ttccgccgcg ctcaaaactt ggtcgctggt gcatttgggc
     9661 ctgctcatgc tgctgtcggc gtactttttc gggcatcttg gcgacatagg cagccccggc
     9721 atttttgtct atggcctgtt cctcatgctg agcatttacg ccatggccga attggccgac
     9781 cggcatcctt tggcctgggt atgggaattg ctcaaaacgg gcctgggctt ttttatcatt
     9841 ttccggcagg gcgattggtt cggaatcaac agcttatggg cttcgggtac ttattttgtg
     9901 gccggatacc tcatcttttc tgcgctgatg gctgttgttt tgaaccgttg gggaaaagtg
     9961 gaaacagcta cgcctactgc tgtctgaaca caataaacac ccgatatttt cgattattga
    10021 gccgaattca aaacttacat gaacatgaga aaatggtacc tgctcttcgt ttcactgctt
    10081 gccgtcatca gcctgcacgc cgccgacggg caccgcatca aagtgaaagt gacgggctac
    10141 gatcagaaag agctgtatct ggcctattac tacggcgaca aacagtatgt gctggatacc
    10201 gccaccttgg ccaatgacgg ctttttccac tttgccgatg agaagccgtt gaatgcggga
    10261 gtttatctcg tggtgctggc gcccgataac tcttacttcc agattttggt agaacctaaa
    10321 gagcaatttt tttcggtttt cacccaaaaa gacaacccat cgtcgaatgt gaagtttgaa
    10381 aagtcgccag ccaatacgca gttttacagc t
//
LOCUS       DAORLF010000125        10397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3435_length_10397_cov_2.791143, whole genome shotgun sequence.
ACCESSION   DAORLF010000125 DAORLF010000000
VERSION     DAORLF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3435_length_10397_cov_2.791143"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..1839)
                     /locus_tag="PK198_11420"
     CDS             complement(436..1839)
                     /locus_tag="PK198_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF39393.1"
                     /translation="MAQAVQIFIAYARKDSALLDEFRVHLRPLERADKLRIWYDGLIE
                     PGAEWEASIKKNLHAADIILLLVSADAIASDYFYDKEVADALDRHRKGSARVVPMIVR
                     PCAWRTTPLAELQALPKDGKPVTSWSDRDEAFTYAAEALWDMAHSIEQQREEKTEQAR
                     REKEATEETRHAAQQAKEEQARRAEQERRQRAEEEKRRKEEEKRREKEAAVGMVLVKG
                     GAFDMGGTPEQKDCRDDEKPVTRVTVDDFYMGKYAVTFDEYDAFCAATGREKPSDSGW
                     GRGQRPVINVSWYDAAAYCNWLSEQQNLTKVYTINGDQVSADWSATGYRLPTEAEWEY
                     AARGGGKAVLFGNGKNVADPQEINFDGSAAYKKAYSVAGEYRGKTVPVGSLNSPNALG
                     LHDMSGNVWEWCWDWKAAYPGGHENNYRGPASGSLRVLRGGSWYNYPRLVRVAYRGSV
                     TPDDRNYGIGFRLSRTP"
     gene            2180..2644
                     /locus_tag="PK198_11425"
     CDS             2180..2644
                     /locus_tag="PK198_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRF39394.1"
                     /translation="MVLDSNFKEFIQSLNANGVKYLVIGGFAVAYHGYPRYTKDIDFW
                     IWASPDNATRVLQTIRDFGFGSLGLTEDDFLDTTNVIQIGQAPNRIDLIMELEGMDFA
                     ECYEHRREVTFEGISLHFIDLDDLIESKLLAGRRKDLVDVDELKKQKKKKKR"
     assembly_gap    2808..2860
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2861..3216)
                     /locus_tag="PK198_11430"
     CDS             complement(<2861..3216)
                     /locus_tag="PK198_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39395.1"
                     /translation="MLLLLLLVACSGSDRHLVVVKNDKPVDLSRLEEVRKHLNGFWVP
                     EDARDEFWKENILWWSFHKDSNYALEYFLPYNDEIKRTETVPIHTDPIHIEPMRRNGQ
                     TYLARVGLGGSDTVQL"
     gene            3263..3466
                     /locus_tag="PK198_11435"
     CDS             3263..3466
                     /locus_tag="PK198_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39396.1"
                     /translation="MKQKKAIMVQVILDSQWFANFSFCKPLAISYPDEKWFIFFRKPF
                     FIGYKYYADVNPPHTVRWNFAQA"
     gene            complement(3480..>4082)
                     /locus_tag="PK198_11440"
     CDS             complement(3480..>4082)
                     /locus_tag="PK198_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF39397.1"
                     /translation="GLVVPAATAPGEIVVNGMSMSRRDSPYANSGVVVAVELEDLAVY
                     SRHGVFAGLEFQKEVEQAMFAWGDGSQRAPAQRLTDFAEGRLSKYLPAGSYIPGLTPA
                     PLHELLPAFIYQRLQQGVRDFGRKMRGYYTSEANVIGTESRTSSPVRIPRRPDTLMHD
                     QVAGLFPCGEGAGFAGGIISAAMDGQNVARAVRKYLEGRG"
     assembly_gap    4083..4095
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4096..5044)
                     /locus_tag="PK198_11445"
     CDS             complement(<4096..5044)
                     /locus_tag="PK198_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF39398.1"
                     /translation="MIRELEIKLLPAEAADPAVIRQRAIQKSGLNPSRIKDVRVLRRS
                     IDARGSRPIVILRVDVFADEPYTPEPALLGLLRPVDTAPAVIIVGTGPGGYFAALELI
                     EAGIKPILFDRGKDVRARRRDLRAIQQDGVVHPHSNYCFGEGGAGTYSDGKLYTRSHK
                     RGDIHKALTLLVEHGATSEILVEAHPHIGSNKLPQIVANIRETILHYGGEVHFDSWVT
                     DLILHQGRAVGVVVNDRDEHLAEAVILATGHSARDVYAMLHRRGVRLEAKPFALGVRI
                     EHSQAFVDEMQYRQSPREEHLPAASYKLITQVNGRGVFSF"
     gene            complement(5054..5497)
                     /locus_tag="PK198_11450"
     CDS             complement(5054..5497)
                     /locus_tag="PK198_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39399.1"
                     /translation="MTRQLFFLLFLLLSLSAFGQQRRVMHQTYDLNGIASVLFDIKDN
                     YEVELWAGTALLIETTVEMWEINAKGKVDAPESIFKHFVDKGRYQTAGALLNGNQLRV
                     QSKDMQRAAIRTKTGECSEEVLVKIMLPDSFQKTGDHIWTRSGKE"
     gene            complement(5507..7297)
                     /gene="lepA"
                     /locus_tag="PK198_11455"
     CDS             complement(5507..7297)
                     /gene="lepA"
                     /locus_tag="PK198_11455"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRF39400.1"
                     /translation="MKNIRNFCVIAHIDHGKSTLSDRLLETTKTLSHRDMKEQTLDDM
                     DLERERGITIKSHAIQMSYLHTDGERYTLNMIDTPGHVDFSYEVSRSIAACEGALLLV
                     DASQGIQAQTISNLYLAIEHDLEIIPVLNKIDMESAMVDEVSDQIIDLIGCKREEILL
                     ASGRTGQGVEDVLAAIVARIPAPKGDPEAPLQALIFDSIFNSFRGVIANIRIMNGTLR
                     HGDKIQFMNTGRNYQADEVGILQMDYVAGKELAAGNVGYVITGIKVSKEIKVGDTITH
                     LQAPAAEPIKGFEDVKPMVFAGIFPLDNDDFEDLRDSLEKLQLNDASLVFEPESSAAL
                     GFGFRCGFLGMLHLEIVQERLSREFNQDVITTVPNVSYNAFTRRGESLIVRNPMDMPD
                     PTVLDYAEEPFIQAQIITKPEYIGTIMTLCMDKRGILTKQNYLTETRVEMTFEMPLAE
                     IVFDFYDRLKSISRGYASFDYQPIDYRLTDLVKLDIKLNGENVDALSALVHRSKAESF
                     GRGICSRLKELLPRQQFVIAIQAAVGAKIIARETISAMRKDVTAKCYGGDISRKRKLL
                     EKQKEGKKKMRQFGKVEVPQKAFLEVLKLN"
     gene            7365..8123
                     /locus_tag="PK198_11460"
     CDS             7365..8123
                     /locus_tag="PK198_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-5-alpha-steroid 4-dehydrogenase"
                     /protein_id="HRF39401.1"
                     /translation="MEAKTFYLLCFIWTALALLLVPIQLFIAAPYGRHVKSTWGPLIN
                     NRLGWMIMEIVSPLAMLGWMWSGAPAASGMLYFLMSLWGLHYLNRSIIFPLRLHTAGK
                     RIPISIVLSAAFFNSVNGFLNGYWWGHFAPPYPPEWVTDPRFMIGAALFVIGAYINHR
                     ADALLIALRKPGDSGYYIPQGWLFRYISCPNHFGEILEWCGFALMAWNLPALSFAIWT
                     AANLIPRALAHHRWYRSHFDDYPSERKAVIPGIL"
     gene            complement(8287..9144)
                     /gene="hisG"
                     /locus_tag="PK198_11465"
     CDS             complement(8287..9144)
                     /gene="hisG"
                     /locus_tag="PK198_11465"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRF39402.1"
                     /translation="MSILKIAIQKSGRLSEKSLQLLKECGIHLPSGDRKLKTEASNFP
                     VEVLYLRDDDIPQYVEQGVVDVGILGENEVWEKGKNVVEVQKLGFAGCRLSLAIPKDE
                     HYAGPEYFQHKRVATSYPRILSRFFEERGISATVEEIGGSVEIAPGIGLADGIFDIVS
                     TGSTLLMNGLKEVEVVVRSQAVLISHPELSEDKRLILNKLLFRVHAVQNASANKYILL
                     NAPNSAIPDIARVLPGMKSPTVLPLVDEGWSSVHSVIPEEAFWEVIDELKRLGAQGIL
                     VIPIEKMVL"
     gene            complement(9320..>10397)
                     /locus_tag="PK198_11470"
     CDS             complement(9320..>10397)
                     /locus_tag="PK198_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF39403.1"
                     /translation="FTVTCKTTAETKRIWKQLAKDGAELMPLQKYPWSALYGWVQDRF
                     GLTWQVTMSSPDSARPGIVPSFLFTGPQRLRAEAAIQRYCEVFGDDSSIKMLSRYEAG
                     GAGPEDSLQYAEFSLAGQTFIAMDNPMPDQKYSFNEAFSFVVTCKNQREVDYFWEQLT
                     AGGGEESMCAWLKDPFGVSWQVVPTALTKALADPDPEKARRAAANMMQMKKIIIRQLR
                     RPLKKTKIEVRTTVKAPIDKVWKYWTSPANIMQWNHASDDWHCPAASNDLRSGGRFSY
                     TMAAKDGSAKFDFEGEYEKIVHNRSIEYVVADGRKVKITFKGKGKRTEIVEVFEAEGI
                     HPHDMQRAGWQAILDNFARHVEAM"
BASE COUNT         2140 a         2880 c         2802 g         2509 t
ORIGIN      
        1 gtattatgcg ggttctaccc gccttgcaac cattcgcaac gggcaagagc tgttagcttt
       61 tacctgctgt tgctccggta aagctaacag ttctccaagt accgctcgca cgagatggaa
      121 tctcgcgcga gtattatgcg ggttctaccc gccttgcaac cattcgcaat ggtcaatatc
      181 tgttagcttt cacccgctat tgccccgaaa agctaacagc tctacacctg caccaatgga
      241 aaccaccaca ctcatatata tacgcccccg atttcgtata tatacattca gcggcgacac
      301 ttggacgctt cgcgtgcata tataggccat atataggcgc tcttttttcc gatatacgga
      361 aatgaaaaag ggaacgatgc aactcgctcc cttttcaggt tttttcaaaa ggtaagaagg
      421 taaaaatgcc ataatctaag gggtcctgga aagacggaac cctatgccgt agttgcgatc
      481 gtcgggagtg acgctgccgc gatacgccac ccgaacgagc cgcgggtagt tgtaccacga
      541 cccgccgcgt agcacacgga gggagcccga agccggcccg cggtagttgt tttcgtggcc
      601 gccggggtag gccgccttcc agtcccagca ccattcccac acattgccgc tcatatcgtg
      661 cagccccagc gcattgggac tgttcaggct gcccacgggt acggttttgc cgcggtattc
      721 gccggctacg gagtaggctt ttttataagc tgcgcttccg tcgaaattga tctcttgggg
      781 gtcggccaca tttttcccat tgccgaacag cacggccttg ccgccgccgc gggccgcgta
      841 ttcccactcg gcctcggtgg gcaggcggta gcccgtagcg ctccagtcgg cgctcacttg
      901 gtcaccattg attgtataaa ctttggtgag gttctgctgt tcgctgagcc agttgcagta
      961 ggctgcggcg tcataccagc ttacgttgat gacaggtcgt tggccgcgcc cccagccgct
     1021 gtcggagggc ttttctctgc ccgtggctgc acaaaaggca tcgtattcgt caaacgtcac
     1081 agcatatttc cccatataaa aatcatccac cgtcacgcgg gtcacgggtt tttcgtcgtc
     1141 tcggcagtct ttttgctccg gagtgccgcc catgtcaaag gctccgcctt ttaccagcac
     1201 catgcctacg gcggcctctt tttcgcggcg tttttcttct tccttgcggc gtttttcttc
     1261 ttcggcacgt tgccgtcgct cctgctctgc gcggcgcgcc tgttcctctt tggcttgttg
     1321 ggcggcgtgg cgggtctctt cggtggcctc tttttcgcgg cgggcttgtt ccgtcttttc
     1381 ctcccgctgc tgctctatgc tgtgtgccat gtcccacaga gcttcggcgg cgtaggtaaa
     1441 agcctcgtcg cggtcgctcc aggaggtaac gggtttgccg tctttgggca gggcctgcag
     1501 ctcggccaat ggcgtcgttc tccaggcgca ggggcgcaca atcatgggca ctacgcgggc
     1561 gctgcctttg cggtggcggt ccaaggcatc ggccacttct ttgtcgtaga aataatcgga
     1621 ggcaatggcg tcggcgctca ccaagagcag gatgatgtcg gcggcgtgca ggtttttttt
     1681 gatgctcgcc tcccactcgg cgccgggttc tatgaggccg tcgtaccata tccgcagctt
     1741 gtcggcgcgc tccaaaggcc ggaggtgtac gcggaactcg tccagcaggg cgctgtcctt
     1801 gcgggcgtag gcgatgaata tttgtacggc ttgggccatg agtggtgcag gcttgcggtt
     1861 gtggggcgta aaggtaggag attttgggga taaaataaac cataaggcgc acaggcagcg
     1921 ggaatccgta ccgactccgc gggctgctca tgtcggcgag taaaatacaa catttcaaaa
     1981 tgctgaaaaa aagctatctt tgtcaagtaa attgatcagg atatggctgt aaatgaatcc
     2041 attcaacaga cggctccact cgtccgtatc ttcaaaaaag gcgaagaacc tgacgacatt
     2101 ttttattggc tgtctcgccc gcccattgaa cgcatctgcg ctttggagga aatacgcaaa
     2161 caatacaacg actggaaata tggtactgga ctcgaatttc aaagagttta tacaatcgtt
     2221 aaacgcaaac ggggttaagt atctcgttat tggcggtttt gcggttgctt atcatggcta
     2281 tccccggtac acaaaagaca ttgacttttg gatatgggcc tcgcctgata atgcgacccg
     2341 agtgttgcaa acgattcggg actttggctt cggctcactc ggcttgacag aggatgattt
     2401 tttagacact accaatgtga ttcaaatcgg ccaagcgccc aatcgaatag atttgatcat
     2461 ggaattggaa ggcatggact tcgccgaatg ttacgaacat cggagggaag ttacttttga
     2521 aggaatctcc cttcatttta ttgaccttga cgaccttatt gaaagcaaac ttcttgctgg
     2581 tcggcgaaaa gatttagttg atgtggatga attgaaaaag cagaagaaaa aaaagaaaag
     2641 atgactttat accgccttgc acgcccctgt ttttcaaaaa acgcatagcg ctcgcaccgg
     2701 catagccagc agccccgctc gcgcgagatt ccatctcgtg cgactatccg gcgggttcta
     2761 cccgccttgc acatccctca tttacttcgg ttgcaaaaac gcatagcnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccaactgca ccgtatccga
     2881 gccgcccagg ccgacgcgcg ccagatacgt ttggccgttc ctgcgcatcg gttctatatg
     2941 gatagggtcg gtgtggatcg gcaccgtttc agtccttttg atttcgtcgt tgtaaggaag
     3001 aaaatattcg agcgcgtaat tcgagtcctt gtgaaaggac caccagagga tgttttcttt
     3061 ccaaaactca tcccttgcgt cttccggcac ccagaacccg ttgagatgct tgcgcacctc
     3121 ctctaaccga ctcaaatcta ccggcttatc gtttttcacc actacgagat ggcgatcaga
     3181 gccggagcag gccacgagca gcagaagcaa gagcaaccat attggccgtg aggtgtagtg
     3241 cagccactta ttttttgtat gtatgaaaca gaaaaaagcg atcatggtgc aagttatact
     3301 cgatagccag tggtttgcga attttagttt ttgcaaacca ctggctatca gttatcccga
     3361 tgaaaaatgg tttatatttt ttcgcaaacc atttttcatc gggtataagt attatgcgga
     3421 cgtgaatccg ccacataccg tcagatggaa ttttgcacag gcatagtcag cagccccgtt
     3481 tagccccttc cttccaaata tttcctcact gcccgcgcca cattttgccc gtccatcgct
     3541 gccgagatga tgccgcccgc aaagcccgct ccctcgccgc agggaaacaa gcccgccacc
     3601 tgatcgtgca tcagcgtatc ggggcggcgc ggaatgcgca ccggcgaact ggtgcggctc
     3661 tctgtgccga ttacattggc ctcggaggtg tagtagccgc gcatttttcg cccgaaatcc
     3721 cgtacgccct gttgcaatct ttggtaaatg aacgcgggca gaagctcatg caggggcgca
     3781 ggcgtcaagc cgggaatgta cgaacccgcc ggcaggtatt tcgacaggcg gccctcagca
     3841 aagtcggtga gccgctgggc cggagcgcgc tggctgccgt cgccccaggc aaacatggcc
     3901 tgttctactt ctttttgaaa ttccagaccc gcaaacacgc catgccggct gtacacggct
     3961 aagtcttcca actctaccgc caccaccacg ccggaattgg catacggaga atcgcgccgc
     4021 gacatggaca tgccgttcac cacgatctcg ccgggggccg tagctgccgg caccaccaag
     4081 ccnnnnnnnn nnnnnaaaac gaaaacacgc cgcgcccgtt cacctgcgta atgagtttgt
     4141 aactggcggc aggcaggtgc tcctcgcggg gcgactgccg gtactgcatc tcatccacaa
     4201 aggcttgcga atgttcgatg cgaacgccca gtgcaaaggg cttggcctct aagcggacgc
     4261 cgcgccggtg cagcatggca tacacatcgc gcgcagagtg gccggtagcc agaatgacgg
     4321 cttccgccag atgctcgtcg cggtcgttga ccaccacgcc cacagcccgg ccctgatgca
     4381 ggatcagatc ggtgacccaa ctgtcgaaat gcacttcgcc gccgtagtgg aggatggttt
     4441 cgcggatatt ggccacaatc tggggcagct tgttggagcc gatgtgcgga tgcgcttcaa
     4501 cgagaatctc cgaagtagcg ccgtgttcta ccaacaatgt cagtgcttta tgaatatcgc
     4561 cgcgcttgtg cgagcgggtg tagagcttgc cgtcggaata ggtgccggcg ccgccctcgc
     4621 caaagcagta gttggaatgg ggatgcacca cgccgtcttg ctgaatggcc ctcaaatcgc
     4681 ggcggcgggc gcgcacgtcc ttgccacgat caaacaggat gggcttgatg cccgcctcga
     4741 tcaactccag cgcggcaaag tacccgcccg gtccggttcc tacaatgatg accgccgggg
     4801 ccgtatccac cgggcgcaaa aggcccagca gcgcgggttc gggcgtgtaa ggctcgtcgg
     4861 caaacacgtc cacgcgcaaa atgacgatgg ggcggctgcc gcgcgcatct atggagcggc
     4921 gcagtacgcg cacgtctttg attcgggagg ggttcagccc tgacttttgg atggcccgtt
     4981 ggcggatcac cgccggatcg gcagcttcgg cgggcagcag tttgatttcg agttcgcgga
     5041 tcatggcgcg cacttattct ttgccggagc gcgtccagat atgatcgccg gttttttgaa
     5101 aactgtcggg cagcatgatt ttgaccaaga cttcctcaga acattcgccg gttttggttc
     5161 tgatggcggc gcgctgcatg tctttagact gaacgcggag ttggttgccg ttgagcaggg
     5221 cgccggcagt ctggtaacgg cctttgtcca caaaatgctt gaagatggat tccggggcgt
     5281 ccactttgcc cttggcgttg atttcccaca tttccacggt ggtttcgatg agcagcgcag
     5341 tgcccgccca tagttccacc tcgtagttgt ctttgatgtc gaacagcacg gaggcaatgc
     5401 cgttcagatc gtaagtctgg tgcatcacgc gccgctgttg cccgaaggcg gaaaggctga
     5461 ggaggaggaa gaggaggaaa aacagttgtc ttgtcatagc aggaaattaa ttcaacttca
     5521 acacctccaa aaacgccttt tgcggcactt ctactttgcc gaactggcgc attttctttt
     5581 tgccctcttt ttgtttttct aacagtttgc gcttgcggct gatgtcgccg ccgtagcact
     5641 tggccgtcac gtctttgcgc atggccgaaa tggtttcgcg ggcaatgatc ttggcgccca
     5701 cggcagcctg tatggcgatg acaaactgct ggcggggcag caattccttg agccgggagc
     5761 aaatacccct gccgaaactc tccgctttgg agcggtgaac gagcgcagag agggcatcca
     5821 cattttcgcc gttgagcttg atgtcgagtt tgaccaagtc ggtgaggcgg tagtctatgg
     5881 gttggtagtc gaaggaagca tagccgcggg agatggactt gaggcggtcg taaaagtcaa
     5941 acacgatttc ggccagcggc atctcaaagg tcatttccac gcgggtttcc gtcaggtagt
     6001 tctgcttggt caggatgccg cgcttgtcca tgcacagcgt catgatggtg ccgatgtact
     6061 ccggtttggt gatgatttgt gcctgaatga acggctcctc ggcgtagtcc agtaccgtcg
     6121 gatcgggcat gtccatcggg ttgcgtacta taaggctttc gccacggcgg gtgaaggcat
     6181 tgtaggatac gttgggtacg gtggtgatca cgtcctgatt gaactcgcgc gagaggcgct
     6241 cctgcacgat ttcgaggtgc aacataccga ggaagccgca gcggaaacca aagcccagcg
     6301 ctgccgagga ttcgggttca aacaccaaag aagcgtcgtt gagttgcagt ttctccaagc
     6361 tgtcgcggag gtcttcaaaa tcgtcgttgt ccaaggggaa gatgcctgca aacaccatcg
     6421 gttttacatc ctcaaaacct ttgatcggct cggctgccgg cgcctggagg tgcgtgatgg
     6481 tgtcgcctac tttgatctct ttcgacacct tgatgccggt gatgacgtag cccacgttgc
     6541 cggcggctaa ttctttaccg gccacataat ccatttgcaa aatgcccacc tcatcggcct
     6601 gataattgcg gccggtgttc ataaactgaa tcttgtcgcc atggcgcagg gtgccgttca
     6661 tgatgcggat gttggcaatg acgccccgaa acgagttgaa aatggagtcg aaaatcaatg
     6721 cctgaagcgg cgcttcggga tcgcctttgg gcgccggaat gcgcgctaca atggctgcca
     6781 aaacatcttc cacgccctgc ccggtacgac cgctcgccag cagaatttcc tcccgcttgc
     6841 aaccaatgag gtctatgatc tggtccgaaa cctcgtccac catggcgctc tccatgtcaa
     6901 ttttgttgag caccggaatg atctccaaat cgtgttcgat ggcgagatac aggttggaaa
     6961 tggtctgagc ctgaatgcct tgcgacgcat ccaccagcaa caaagcgccc tcgcaggcag
     7021 caatggagcg cgacacttcg taggagaagt ccacgtggcc gggggtgtct atcatgttga
     7081 gcgtatagcg ctcgccgtcg gtgtgcaggt aagacatctg gatggcgtgg ctcttgatgg
     7141 tgatgccgcg ctcgcgttcc agatccatgt cgtccaaggt ttgttccttc atgtcgcggt
     7201 gcgagagcgt tttggttgtt tccaaaagcc tgtctgacag ggtgctcttc ccgtggtcaa
     7261 tgtgtgcgat gacgcaaaaa tttctgatat tcttcatagg gcgcaaagat acggcgaaat
     7321 gcaaatacat aatgaatgat aggaaaacat tttacctttc gccaatggaa gcaaaaacgt
     7381 tttacctgct gtgtttcatc tggacggccc tcgccctcct tttggtgccc attcaattat
     7441 tcatcgcggc gccgtacggg cggcacgtca aatctacctg ggggccgctc atcaacaacc
     7501 gcttaggctg gatgatcatg gaaatcgtgt ctcccttagc catgctgggc tggatgtgga
     7561 gcggcgcgcc ggcggcatcc ggcatgttgt actttctgat gagcctctgg gggctgcatt
     7621 acctcaaccg gagcatcatt tttccgctgc ggctgcacac cgcaggcaaa cgcatcccga
     7681 tttccatcgt gctgtcggcg gcatttttca actccgtcaa cggctttctc aacggctatt
     7741 ggtgggggca tttcgcgccg ccttaccctc ctgaatgggt aacagacccc cgatttatga
     7801 tcggcgcggc actgtttgtc atcggcgcat atatcaatca ccgggccgat gccctcctca
     7861 tcgccctccg aaaaccgggc gacagcggct actacatacc acagggttgg ctgtttcgct
     7921 acatttcatg ccccaaccac ttcggcgaga tattagaatg gtgcggcttt gcgctcatgg
     7981 cctggaatct gcccgcactc agtttcgcca tctggacggc ggccaacctc attccgcgcg
     8041 cattggccca ccaccgttgg tatcgttcgc atttcgacga ttatccgtca gaacgcaaag
     8101 cggtgattcc gggaatatta taattttttt tgactggggc gtgccggcgc tctgactgcc
     8161 tgcaatgtcc gggcagcatc aggtggcgcc gtcgggtgcc tcagtcgctc ctcgggtcgc
     8221 tcccttcggc cccctcacgc atgcaaaccg ataacgctca ttggatcgcg gagactccac
     8281 cctccctcac agcaccatct tctctatcgg gatcaccaaa ataccctgtg cgcccaatcg
     8341 cttcagttca tcaatcactt cccaaaatgc ctcttccggg atgaccgaat gcacgctgct
     8401 ccagccctca tccaccaatg gcagcacagt ggggcttttc atgcccggca gcacccgcgc
     8461 tatgtcgggg atggcgctgt tgggcgcatt gaggaggatg tacttattgg ccgaggcgtt
     8521 ttgtacggca tggactctga aaagtaattt gttcagaatg agccttttgt cttcgctcag
     8581 ttccgggtga ctgatgagca ccgcctggct gcgcaccacc acttccactt ctttgaggcc
     8641 gttcatcagc agagtgctgc ccgtactcac gatgtcgaaa atgccgtcgg ccagaccgat
     8701 acccggcgct atttccacac tgccgccaat ttcttccacc gttgccgaaa tgccccgttc
     8761 ttcaaaaaaa cgactcagaa tgcgcggata actggtcgcc actcttttgt gctgaaaata
     8821 ttccggcccg gcatagtgct cgtctttggg aatggccagc gacaggcggc atccggcaaa
     8881 accgagcttt tgtacttcta ccacattttt gcctttttcc cacacttcgt tttcgcccag
     8941 aatgcccaca tcgaccacac cctgttccac atactgcgga atgtcgtcat cgcggaggta
     9001 cagcacctcg acgggaaaat tggaagcctc ggttttgagt ttgcggtcgc ccgaaggcag
     9061 atgaataccg cactctttca gtagttgcag cgatttttca ctgagccgtc cgctcttttg
     9121 gatggcaatt ttcaggatac tcatgcttga ttttgttggg aaagcgcacc cgcggacgaa
     9181 atgacggaaa agattacata ggggcgagaa aaaaataaag tgatcatttt cgccaaagga
     9241 ttagataagt aaacgtttca ttttaagtgt cttatctaat cctttggcct aaagaaaatg
     9301 agcatttatt tttgccccac tacatagcct ctacatgccg ggcaaaattg tccaaaatgg
     9361 cctgccagcc ggcgcgctgc atgtcgtgcg gatgtatgcc ttccgcttca aaaacctcca
     9421 ctatttccgt ccgcttgccc tttcctttga aggtgatttt tacttttctg ccgtctgcaa
     9481 caacgtattc aatgcttctg ttgtggacaa ttttttcgta ttcgccttca aaatcaaact
     9541 tggcactgcc atctttggcg gccatcgtgt agctgaaccg gcctccggag cgcaagtcgt
     9601 tggaagctgc cgggcaatgc cagtcgtcgg aggcgtgatt ccattgcatg atgttggccg
     9661 gcgaggtcca gtatttccat accttgtcta tcggtgcttt tacagtcgtc ctcacctcta
     9721 ttttcgtctt tttcagcggt cgccgaagtt gccggatgat gatctttttc atttgcatca
     9781 tattggcggc agcgcggcgg gctttctcag gatcagggtc ggccagggct ttggtgagcg
     9841 cggtgggtac cacctgccac gacacgccaa aggggtcttt gagccaggcg cacatacttt
     9901 cttcgcctcc gccggccgtg agttgctccc agaaatagtc cacctcgcgt tggtttttgc
     9961 aagtcaccac gaacgaaaac gcctcgttga aactgtattt ctgatcgggc atggggttgt
    10021 ccatagcaat gaaagtctga ccggctaagc taaactcggc gtattgcagc gaatcttccg
    10081 gccccgcgcc gcccgcttcg taccgcgata acattttgat ggaggaatca tcgccgaaaa
    10141 cttcgcaata ccgttgaatg gcagcctcgg cccgtaggcg ttgcggcccc gtgaacaaaa
    10201 aggacggcac tataccaggc cgggcgctgt cggggctgct catcgtgact tgccaggtga
    10261 ggccgaaacg atcctgcacc cagccataca aggcgctcca ggggtatttt tgcagcggca
    10321 tcagttcggc gccgtctttg gcaagctgct tccagatgcg cttggtttct gctgttgttt
    10381 tgcaggtgac ggtgaac
//
LOCUS       DAORLF010000126        10364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3450_length_10364_cov_2.207295, whole genome shotgun sequence.
ACCESSION   DAORLF010000126 DAORLF010000000
VERSION     DAORLF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_10364_cov_2.207295"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..294
                     /locus_tag="PK198_11475"
     CDS             19..294
                     /locus_tag="PK198_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39404.1"
                     /translation="MSYLILWPKENERFTFAPLLNKRQIYFFRVAMGFLQNVVATLKK
                     NYTVKGAQQKKSAYHCSMELPENPEQSTERMPKSLESRQNPFYLNIL"
     gene            291..>509
                     /locus_tag="PK198_11480"
     CDS             291..>509
                     /locus_tag="PK198_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39405.1"
                     /translation="MKKSILLQFVLMLFVAGNIQAAANLALPEDVKNQPEISEIISET
                     GQSDVEAFLNLTPSKYKEMTGEKMGLKKT"
     assembly_gap    510..587
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <588..808
                     /locus_tag="PK198_11485"
     CDS             <588..808
                     /locus_tag="PK198_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRF39406.1"
                     /translation="SAAGAKSQLVALILVIFVGVLGIHRFYLGYTVIGIIQLLTAGGC
                     GIWALIDLIRIITGELKPADGSEYNPTL"
     gene            831..1109
                     /locus_tag="PK198_11490"
     CDS             831..1109
                     /locus_tag="PK198_11490"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRF39407.1"
                     /translation="MGVFIFVPLVLLLMPADQFDEGPPLCMSVILFKQECYACGLTRA
                     VQHLIHFQFEEALYYNPLVFVVFPLLAFLWAKWFITDVKKIHQIRQDS"
     gene            1267..2043
                     /locus_tag="PK198_11495"
     CDS             1267..2043
                     /locus_tag="PK198_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39408.1"
                     /translation="MTFARQNHLFFITIIPVAMRIHILTLLLVFGLYNLAGAQPDRLI
                     NDQNFTRLAFASPGSHGLITTNRPQLHIENTPFYNENWLPGRVKIIGHDTFSRWAGVI
                     LELDKGELYVQLEEDFISNFPLRWVNLLQIDESGKITTFMVRDLFSDHNAGPKGHKFY
                     EIIHLGEKYTLLKCHTKFLRKEDYVENLGMVRRPNKYMELYSYWVLAGNSLRPAGANK
                     TAMKKAIPEKANRIDSYVKSKKLQLKKKEGLAQLMQLLEK"
     assembly_gap    2119..2128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2129..4288
                     /locus_tag="PK198_11500"
     CDS             <2129..4288
                     /locus_tag="PK198_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HRF39409.1"
                     /translation="AMDCGPSCLRMVAEHYGRSYTLQYLRERSHIDREGVSLLGIAEA
                     AERIGLRTLAYKLPFKGESDEDPGLTDAVLPCIVHWKQQHFVVVYGISKNHVKVADPA
                     LGKLKLTHTEFRNGWLSDGAKGVALLVETTPEFFDQAGDEREKGFRLGFLMRYLQPYK
                     KLLTQLGLGLLMLSMFQLAFPFLTQSIVDIGIDNADLRFIYLILAAQLMLFLGLTSVN
                     ILQAWILLHISTRVNVALIADFLTKLMRLPIGFFDSKMTGDLLQRIGDQRRIESFLTG
                     STLNLLFSVINLLVFGSVLMFYHWPVFVLFLVSAALYFGWIALFLPRRRVVDQKRFAE
                     HSENQSALIELIQGMQEIKLQRSERRHRFHWAQIQAKLFRTNIRGLALSQTQDLGAAF
                     ISQLKDIFITFIAARAVISGEITLGMMLAMQFIIGQLNGPLFQLAGFIRSAQDARISL
                     ERLGEIQDMEDEYQPETVADEMRPEIGDIRLENVSFRYNELNDYVLQDITLDIPLGKV
                     TAIVGSSGSGKTTILKLLLGFYTPSKGSIYVGNAHLKHVSPRAWRGVCGAVMQDGFIF
                     SDTIARNIAQSDETPNKRRLWESVRIAQAQEFIEALPLGYNTIVGSRGNGLSQGQRQR
                     LLIARAVYKNPHFLFFDEATNALDAENESRIVHQLEEFYRGRTVMVVAHRLSTVRHAD
                     QIVVLEKGRIVERGTHEQLTASRGYYYRLIKNQLELG"
     gene            4449..5297
                     /locus_tag="PK198_11505"
     CDS             4449..5297
                     /locus_tag="PK198_11505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRF39410.1"
                     /translation="MAQPRKPKQERNTAKTAKKRPSTSFSARRRPELLKNMEPSEDQW
                     PMRLNKYIAHCGICSRRQAADYVKGGLVAVNGQVITEPGVEVQQTDTVTYKGQNIAPE
                     LRKVYILMNKPKNVITTSEDERGRKTVLNLLEGKIRERVYPVGRLDRDTTGLLLLTND
                     GDLAQKLSHPKHKVVKVYKVTLNKSLQIKDFEAIGAGLDLEDGKAEVDSIYYYEGKKS
                     EIGVEIHSGKNRIVRRIFEHLGYNVEALDRIYYAGLSKKDLPRGQFRRLSEREVIQLK
                     HFTETK"
     gene            complement(5303..5686)
                     /locus_tag="PK198_11510"
     CDS             complement(5303..5686)
                     /locus_tag="PK198_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRF39411.1"
                     /translation="MKYTVDKQERYTIFRLDETTLNSVLAPQLKSEFVILSNEGVNNL
                     ILDLSNVEFVDSSGLSAILTGDRLWKLIGSFILTGIEHPSVKKLIEISRLETILTIVP
                     TASESIDYIFMEEMERELGVEGEEA"
     gene            5955..>7899
                     /locus_tag="PK198_11515"
     CDS             5955..>7899
                     /locus_tag="PK198_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRF39412.1"
                     /translation="MNNQKFSPKVKQIIARSRQAALRLGHDFIGTEHILMSIIEDRNS
                     LAVRVLESLEIPADELRFKIRESIQQLPASPTMLNVGTLPLNKHAERVLKVTLLEAKS
                     LGSDEIAPEHLVLSILKHEDFPASKILHQFDIDYDMYKAELEYARQEQDFGNAGFIEP
                     FAQAPAEQDDPYDDDENQSRYQQRRSGGNPKSRTPVLDNFGRDVTKLAEEDRLDPIIG
                     RENEIERVSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRTLFNKRIVML
                     DMAAMVAGTKYRGQFEERMKAIMNELEKNRDVILFIDEIHTIVGAGGATGSLDASNIF
                     KPALSRGELQCIGASTLDEYRQYIEKDGALDRRFQKVLVEPPSAEEAVHILHNIKPKY
                     EEFHNVSFTDEAIAACVRFSDRYITDRFLPDKAIDVLDEVGARVHLKNIHVPKHIEEM
                     EKAIEEVKEQKNGAVKNQQYEKAADLRDRESKMARQLEFAKIQWEEESKTKRYPVSEE
                     DIAEVVSMMTGIPVKRVAQSESKKLVGMGKDMEGMIIGQDEAIAKVVKAIQRNRVGLK
                     DPSKPIGSFIFLGPTGVGKTEMAKALARYLFDSEDALIRIDMSEYMEKFTVSRLIGAP
                     PGYVGYEEGGQLTEKVRRKPYSVV"
     assembly_gap    7900..7927
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7928..8531
                     /locus_tag="PK198_11520"
     CDS             <7928..8531
                     /locus_tag="PK198_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39413.1"
                     /translation="AHPDVYNILLQVLDDGQLTDGLGRKVDFKNTIIIMTSNIGVRQL
                     KDFGQGVGFSTKAKQEAAEENSRGVIQNALKRTFSPEFLNRIDDVVIFNSLGQDEILS
                     IIDISLRDLYKRLQGLGYTLTLSEEAKKFVADKGFDPQFGARPLHRAIQKYVEDPLAE
                     FILNENLQEGAILRADLNTETQLLEILVDSSTGVESDVKE"
     gene            complement(8533..8829)
                     /locus_tag="PK198_11525"
     CDS             complement(8533..8829)
                     /locus_tag="PK198_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39414.1"
                     /translation="MSKLADEYARALALAPWLPQMPALLDAVVPVLHDGKYWLADRDG
                     KSCIIVTDDLGGRKLLAVSGGHPVGLFGVWDAGSFRVLSVVSSREDRPPRIVQW"
     gene            complement(8793..9920)
                     /locus_tag="PK198_11530"
     CDS             complement(8793..9920)
                     /locus_tag="PK198_11530"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRF39415.1"
                     /translation="MRTELPGKILFHRTFAPHSTHRMFIWTPDRIRSIAPDENTWERA
                     RRLAHPPVWRNQSGNEAMVWGECKGSGGSFYQTAIDWNGPAYRCSCPVKRMPCKHILA
                     LMLLLHNYSDHFRVTHDLPDWAEQWRAARLKRQAKSASPEQEANRSAQREKKRDQRLD
                     VMAAGIEELDKWLRDMVRQGIAEAENRPASFWEQTAARMTDAKLGSIARRLRMPAFPV
                     AGQSRHLQILNELGDLFLLVEAFRKLDQLPEGMQQDVLTACGVAQRKSDLDGQPGISD
                     RWVVMGQEEGEEEQLRFRRVCSGVKPAGVLRFCSILPGATPALKDTGPPALFLPARCA
                     IFLLNIHSGPWRAPCRMQIFQITTYSLTIPCRNWPTSMPGP"
     gene            10135..>10364
                     /locus_tag="PK198_11535"
     CDS             10135..>10364
                     /locus_tag="PK198_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39416.1"
                     /translation="MTTIGTYFHRAGAQPVNNLVRDVVLPQPVQIIAQLPITGPDQAL
                     TVVVTRRRPLLAMKFRYEPNGNIRELQWKVTR"
BASE COUNT         2590 a         2684 c         2604 g         2370 t
ORIGIN      
        1 gtaaacggtt cattttaagt gtcttatcta atcctttggc ctaaagaaaa tgaacgcttt
       61 acttttgccc cactactaaa taagcggcaa atatactttt ttcgcgttgc aatgggcttt
      121 ttgcaaaatg ttgttgcgac attaaagaaa aattatacgg taaaaggtgc acaacaaaaa
      181 aaatctgcat atcattgcag catggagtta ccggaaaatc ctgaacagag tacggagcgg
      241 atgccgaaaa gcttagagag taggcaaaac cctttttatc tcaatatttt atgaagaaaa
      301 gtattttact tcagttcgta ttgatgctct ttgtagcagg caacattcag gctgcagcca
      361 atctggcatt accggaagat gttaaaaatc aaccggaaat cagcgagatc atctctgaaa
      421 ccggacaatc ggatgtggag gcgttcctga acctgacgcc ctccaagtac aaagagatga
      481 cgggcgaaaa aatgggtctc aaaaagaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga gcgctgcggg
      601 ggctaagagc caattagtcg cgctgatact ggtgattttt gtcggagtac tcggtatcca
      661 ccgtttttat ctcggatata ccgtcattgg tatcatacag cttttaactg ccggcggctg
      721 cggaatttgg gcattaattg acctgattcg cattattacg ggagaactta aaccggcaga
      781 cggtagtgag tacaacccaa cgctctaaaa attatcggat atacatacgt ttgggggtct
      841 tcatcttcgt gccgctggtg ttgttgttaa tgccggccga ccagtttgat gaaggccctc
      901 cgctttgtat gtctgttatc ctgttcaagc aagaatgcta tgcctgtgga ttgacacggg
      961 ccgtacagca tttaatacac tttcaattcg aagaagcgct gtattacaat cctttggtat
     1021 ttgttgtgtt tcctttgctt gcttttctgt gggcaaaatg gttcataacc gatgtaaaaa
     1081 aaattcatca aattcgccaa gattcttaat cagggtattt ttcagcctgt cattcattct
     1141 cgcttaaagc tacccgcatc catcgtctgg atgcgggtat tttattacat tatgtctgct
     1201 gtctgtctcg gttttgtata tttgccttga tttgtactcg cgcagaagtc agcgataaaa
     1261 ctgtaagtga ctttcgcaag gcagaaccat ttatttttca tcacaattat ccctgtcgct
     1321 atgagaattc acatcttgac attactgttg gtgtttgggc tttacaactt ggccggcgcg
     1381 caacctgacc gcctcatcaa tgatcaaaac tttaccagac ttgcatttgc atctccggga
     1441 tctcatgggc tgataactac caacagacct caattgcata tagaaaacac tccattttat
     1501 aatgaaaact ggctgccggg cagggtcaaa attatcggcc atgatacatt cagtcgttgg
     1561 gccggtgtaa tacttgagtt agataaagga gagttatacg tccagttgga ggaagatttt
     1621 atcagcaatt tcccccttcg atgggtcaat ttgttgcaga tagatgaaag tggcaagata
     1681 accaccttta tggtgcgcga tctgttttcg gatcacaatg ccgggcctaa aggccataaa
     1741 ttttacgaga taatacactt gggtgaaaaa tatactttgc tcaagtgcca caccaaattt
     1801 ctgcgcaaag aggattatgt ggaaaacctg ggcatggtcc gccgccccaa taagtatatg
     1861 gaactgtata gctactgggt gctggcggga aattcattgc ggcccgccgg cgccaacaag
     1921 acggctatga aaaaagctat tcccgaaaaa gccaatcgaa ttgattcgta cgtcaaatca
     1981 aaaaaactac agcttaaaaa gaaagaaggt ttggcacaac tcatgcaact gctggaaaaa
     2041 taaggctcgt caatcggttt tagatgattg ctgactgaca ctttccgcga tggacagatt
     2101 tcccttcttc cgacaattnn nnnnnnnngc catggactgc gggccgtcct gcctgcgcat
     2161 ggtggccgag cattacgggc ggagctatac cctgcaatac ctccgcgaac gcagccatat
     2221 tgaccgggaa ggggtttcat tgctcggcat tgcagaggct gccgaacgca tcggcttgcg
     2281 cacgctggca tacaaactac cgtttaaagg tgagagtgac gaagaccccg gcctcaccga
     2341 cgctgtattg ccctgcatcg tgcattggaa acaacagcat tttgtggtgg tatatggcat
     2401 ctccaaaaac catgtgaagg tagccgaccc tgctttgggc aagctcaaac tgacgcacac
     2461 cgagtttcgc aacggctggc tcagcgatgg cgccaaaggg gtggcacttc tggtagaaac
     2521 caccccggag ttttttgacc aagccggcga tgaacgcgaa aagggcttca ggctgggctt
     2581 tctcatgagg tatttgcagc cttataaaaa actgctcacc caattgggct taggcttgct
     2641 catgctcagc atgtttcagt tggcctttcc gtttctcact caatccatcg ttgatattgg
     2701 tatagacaac gccgatctcc gcttcattta cctcattctg gccgcgcaac tgatgctgtt
     2761 tctggggctt accagcgtca acatcttgca ggcttggata cttctgcaca tcagcacccg
     2821 ggtgaatgtg gcattgatag ccgactttct gaccaagctc atgcgtctgc ctatcggctt
     2881 tttcgacagc aaaatgacgg gcgacctgct tcagcgcatc ggcgaccaaa gacgcataga
     2941 gtcgtttctc acaggctcta cgctcaatct cttgttttcg gtcatcaacc tgttggtctt
     3001 cggctccgta ctcatgtttt atcattggcc ggtatttgtg ttgttcctcg tttcggctgc
     3061 cctgtacttc ggctggatag ccctgttttt gcctcgccgc agggtggtag atcaaaagcg
     3121 atttgccgaa cattctgaaa atcaaagtgc tctgatcgaa ctgatacagg gtatgcagga
     3181 aataaagctt caacgcagcg aacgcagaca ccggtttcat tgggcgcaga ttcaggcaaa
     3241 gctgttccga accaacattc gtggcctggc actgagccaa actcaggacc tcggcgcggc
     3301 attcatcagc caactcaaag acatttttat tactttcatt gcggcccgcg ccgttatttc
     3361 cggcgagatc acgctgggga tgatgctggc catgcagttc atcatcggcc agctcaacgg
     3421 gcctttgttt cagttggcgg gcttcattcg ttccgcccag gacgcccgta tcagcctcga
     3481 gcgcctcggt gaaatacagg atatggaaga tgaataccag cccgaaacag tggctgatga
     3541 aatgcgcccc gaaataggcg atattcgttt ggaaaacgta tccttcaggt ataatgaact
     3601 gaatgactat gttttgcaag atattacact tgatattcca ttaggcaaag tgacggccat
     3661 agtaggctcc agcggcagcg gaaaaacaac cattctcaaa ttgttgctcg gattttacac
     3721 cccgtcgaaa ggctctattt atgtgggcaa tgcacacctc aaacacgtca gcccgcgtgc
     3781 ctggcgcggc gtgtgcggcg cggtgatgca ggacggattt attttttccg acaccattgc
     3841 ccgcaatatt gcccaaagcg atgaaacgcc caacaaacga cgcctctggg aatcggtccg
     3901 catcgcacag gctcaagagt ttatcgaagc cctgccctta ggctacaaca ccattgtcgg
     3961 aagccggggc aacggcctga gccaaggtca acgacaacgc ctgctcattg ccagagccgt
     4021 gtataaaaat ccgcactttt tgttttttga cgaagccacc aacgcattgg atgctgaaaa
     4081 tgaaagccgc atcgtacatc agttggagga gttctaccgg ggccgcaccg taatggtggt
     4141 cgcgcaccgc ctcagcaccg tacgacatgc cgaccagatt gtggtactcg aaaaagggcg
     4201 catcgtagag cgcggcaccc acgagcaact cactgcctcg aggggctatt attaccgcct
     4261 gattaagaat cagttggaac tgggataaac gcaacgtcag tccactacaa ctgcgtttat
     4321 tccgggggat catgccccgt gacccacagg ccattttgtg cctccctgaa aagttcatac
     4381 atttgtgcct cgaatgcgcc gcagcggagg gagttccttc gtttttcggg catgttttca
     4441 aaaatcagat ggcacaacct cgtaaaccta agcaggagcg caacacggcc aagaccgcca
     4501 aaaagcgccc ttccacctct ttcagcgccc gccgccgacc ggaattgctc aaaaacatgg
     4561 agccatccga agatcagtgg cccatgcgcc tcaataagta cattgcacac tgcggtatat
     4621 gctcgcgccg tcaggccgcc gattatgtaa agggtggctt ggtggccgtc aacgggcagg
     4681 tcattaccga gccgggggtg gaagtacagc aaacagatac ggtcacttac aaaggccaga
     4741 acatagcgcc cgaactccgg aaggtgtata ttttgatgaa taaacccaaa aatgtgatca
     4801 ccacttcaga ggacgagcgg ggtcgcaaaa cggtgctcaa tttgctggaa ggcaaaatca
     4861 gagagcgcgt ttaccccgtg ggccgcctcg accgcgacac caccggcctg ctcctgctga
     4921 ccaacgacgg cgacctggcc caaaaactct cgcatcctaa gcacaaggtc gtcaaggtgt
     4981 ataaagttac tttgaataaa tcccttcaaa tcaaggactt cgaggccatt ggcgcggggc
     5041 tggacctgga agacggcaag gccgaagtgg acagcatata ctactacgaa ggcaaaaaaa
     5101 gtgaaatcgg cgtggagatt cacagcggca aaaaccgcat tgtgcgccgt attttcgagc
     5161 atttaggata caacgtagaa gctttggatc gcatctacta cgcggggttg agcaagaaag
     5221 acctcccgcg cggccaattc cgtcggctca gcgaacggga ggtcattcag ttgaaacact
     5281 tcactgaaac gaaatagggt gctcaggctt cttcgccctc cacgcccaat tcgcgctcca
     5341 tttcttccat aaaaatgtaa tcaatagatt ccgacgcagt gggaacgatg gtgagtatcg
     5401 tttccaaacg ggaaatttcg atcagctttt tgacgctggg atgctcgatg cctgtcaaaa
     5461 taaacgagcc gatgagtttc cacagccggt cgcctgtcag aatggcacta aggccggaag
     5521 aatccacaaa ttctacgttg gacaaatcca gaatgagatt gttgacgcct tcgttggaaa
     5581 gaatgacaaa ctcggacttg agctggggcg ccaaaacaga attgagtgtt gtttcatcca
     5641 atcggaagat ggtgtaacgc tcttgtttat ctacggtgta cttcattatt ggaacgtttt
     5701 tcaaaaggtc ggcgcaaagt taaacaatct gcgacatttg accaacacat cgtttgttgt
     5761 aaaccccgta cctgattttt tgcaggatta aacttcaaga gtgcctccga tgtttatgca
     5821 acattgcaaa aaaacggcat cattttttct ggaggattca gcggtttatt ggccaaaatg
     5881 agcaaccttt gcatggtgct cccaaagtac ctccattatt catcccgaaa gcgtacgcgc
     5941 agcaatcttt tcttatgaat aatcaaaaat tctctcccaa agtcaagcag atcattgccc
     6001 gcagccgcca ggcagccctg cgtctgggcc atgatttcat tggcaccgaa catatcctga
     6061 tgagcatcat cgaagaccgc aacagccttg cggttcgggt gctcgaatcg ctcgaaatcc
     6121 cggcggatga gctgcgtttc aagatcaggg agtccatcca gcaattgccg gcttcgccga
     6181 cgatgctcaa tgtgggcacg ttgccgctca ataagcacgc cgagcgcgtg ctcaaagtaa
     6241 ccctgctgga agccaaatcg ctgggcagcg acgaaatagc ccccgagcat ttggtgttgt
     6301 ctattctcaa acatgaggat tttccggctt ccaaaattct ccatcagttc gacattgact
     6361 acgatatgta caaggccgaa ctggagtatg cccgtcagga gcaggatttc ggcaatgcag
     6421 gcttcataga gccgtttgct caggcgcccg ccgaacagga cgacccctac gatgacgatg
     6481 aaaaccagtc gcgctaccaa cagcggcgca gcgggggcaa tcccaaatcg cgtacgccgg
     6541 tactcgacaa tttcggcaga gatgtaacca aattggccga agaagaccgc ctcgatccca
     6601 tcatcgggcg cgaaaacgag atcgagcgcg tatcgcaaat tctcagccgt cgcaagaaaa
     6661 acaacccgat tctcatcggc gagccgggcg taggcaaaac tgccatcgta gagggcctgg
     6721 cactgcgcat cgtgcagcgc aaggtatcgc gcacgctgtt caacaagcgc atcgtcatgc
     6781 tcgacatggc ggctatggtg gcgggcacca agtatcgcgg gcagttcgaa gagcgcatga
     6841 aggcgattat gaacgagctg gagaaaaacc gcgacgtcat tctttttatt gatgaaatcc
     6901 ataccattgt aggcgcgggc ggcgctaccg gctcgctcga tgcttccaat attttcaagc
     6961 cggcgctctc ccgcggcgaa ttgcagtgca tcggagcatc cactttagac gagtaccgcc
     7021 agtacatcga aaaagacggc gctttggatc gtcgtttcca aaaggtactg gtagagccgc
     7081 catccgccga agaggccgtt cacatcctgc acaatatcaa gccgaaatac gaagaattcc
     7141 acaatgtcag ctttaccgat gaggccattg cggcttgtgt tcgtttcagc gaccgctaca
     7201 ttaccgaccg cttcctgccc gacaaagcca ttgacgtttt ggacgaagta ggcgcccgcg
     7261 ttcacctcaa aaatatacat gtgcccaaac acatcgaaga gatggaaaag gccatagagg
     7321 aggtgaaaga gcagaaaaac ggcgcggtga aaaaccagca gtatgaaaaa gccgccgacc
     7381 tgcgcgaccg ggagtcgaaa atggcccggc agttggagtt tgccaaaata cagtgggaag
     7441 aggagtctaa aaccaagcgc tacccggttt cagaagagga cattgccgag gtggtgtcta
     7501 tgatgacggg cattccggtg aagcgcgtgg cccaaagcga aagcaaaaag ctcgtgggca
     7561 tgggcaaaga catggagggc atgattattg gtcaggatga agccattgcc aaggtggtga
     7621 aagccattca gcgcaatcgc gtgggcctca aagacccttc caagcccatc ggttcgttca
     7681 tcttcctcgg acctaccggc gtgggcaaaa ccgagatggc caaagcgctg gctcgctacc
     7741 tgttcgactc agaagacgcg ctcatccgta tagacatgag cgaatacatg gaaaaattca
     7801 ccgtgagccg tctcatcgga gcgcctccgg gatatgtggg ctatgaagaa ggcggccaat
     7861 tgaccgaaaa agtgcgtcgc aagccgtact ccgttgtgcn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnagc gcatccggac gtgtataata ttctgcttca ggtacttgac gacggacaac
     7981 tcaccgacgg actgggccgc aaggtggatt ttaaaaatac catcatcatc atgacctcca
     8041 acatcggcgt acgccagttg aaggacttcg gtcagggggt cggtttttcc accaaagcca
     8101 agcaggaagc cgccgaggaa aattcccgcg gggtcattca gaacgctctc aagcgcacgt
     8161 tctcgcccga atttctcaac cgcattgacg atgtagtcat tttcaacagc ttgggccagg
     8221 atgagatttt gagcatcatt gatatttcgc tcagagacct ctacaaacgc ctccagggat
     8281 tgggttacac ccttacgctg agtgaagaag ccaaaaagtt cgttgccgac aaaggtttcg
     8341 atccgcagtt cggcgcgcgt ccgctgcacc gggccattca gaaatacgtt gaagacccgc
     8401 tggcggaatt catcctcaac gaaaacctgc aggaaggcgc catacttcgc gccgatctga
     8461 acacagaaac acaactgctt gaaattctgg tggacagcag caccggggtc gaaagtgatg
     8521 tgaaagagta atttaccact gaacgatgcg tggtgggcgg tcttcccggc tgctcaccac
     8581 gctcaatacc cgaaacgacc ccgcatccca cacgccaaac aggccgaccg gatgcccgcc
     8641 gctgacggcg aggagttttc tgccgccaag atcgtctgtt acaatgatac agcttttccc
     8701 atcccggtcg gccagccagt attttccatc atgcagcaca ggcacaacgg cgtccaacag
     8761 cgccggcatt tgcggcagcc agggggctaa ggctaaggcc cgggcatact cgtcggccaa
     8821 tttcgacaag gtatggtaag cgagtaagtt gtgatctgaa atatctgcat ccgacatggc
     8881 gcgcgccacg gcccgctgtg gatattgaga aggaaaatag cacatctcgc cggcaaaaac
     8941 agcgccggtg ggccagtatc cttcaaagcc ggtgtcgccc caggcaaaat cgagcaaaag
     9001 cgcaaaacgc cggctggttt caccccagag cagactcggc ggaacctcaa ttgctcctcc
     9061 tcgccctcct cttgccccat caccacccat cggtcggaga tgccgggctg cccgtccaga
     9121 tcggatttgc gttgcgcgac gccgcaggcc gtcagcacat cctgttgcat gccttccggc
     9181 aactgatcca atttcctgaa cgcctcaacg agcaaaaaca ggtctcccag ttcgttcaaa
     9241 atctgaagat gccgtgactg tcccgctacc ggaaaagccg gcattctcag ccggcgggcg
     9301 atgctgccga gtttggcgtc ggtcatgcgg gcggcggttt gttcccaaaa agatgccggt
     9361 ctgttttctg cttccgcaat gccctgccgc accatatcgc ggagccattt gtccaactct
     9421 tcgatgcccg cggccatgac gtccaatcgc tggtcgcgtt ttttttcgcg ttgagcgctg
     9481 cggttggcct cctgttcggg tgaggcggat ttagcctgtc gtttcagtcg ggcggcgcgc
     9541 cattgctcgg cccagtcggg caggtcgtgg gtgacgcgga agtggtcgga gtagttgtgc
     9601 aacagcagca tcagagccag gatgtgcttg cagggcatgc gtttgacggg gcaggaacag
     9661 cgataggccg ggccgttcca gtcaatggcc gtttggtaaa aagagcctcc gctgcctttg
     9721 cactcacccc agaccatggc ctcattgccc gactgattgc gccacacggg cggatgagcc
     9781 agccggcggg cgcgctccca agtgttttcg tcgggagcga tggagcggat gcggtcgggt
     9841 gtccagataa acatgcggtg agtggaatga ggtgcaaagg tacgatgaaa aaggattttg
     9901 ccgggcaact cggttcgcat gacgttgaaa ggcgcaggtt tttgtgatgg ttgtgccccc
     9961 caaatgttaa acccccaaaa aacttccccc accctcttga ctttttaaaa attcgcccct
    10021 actttcgcag cagttcttta actcgaaaaa tttttgacct catgtagaag attattttgc
    10081 cctccccctg tttggagtgc tttaaatttt aagcaaattc ttttactttg cctcttgact
    10141 accatcggca catatttcca tcgagcaggc gcgcagcccg tcaataattt ggtgagagat
    10201 gtggtgctgc cgcaacccgt gcagatcatc gcgcagctcc ccatcaccgg ccccgatcag
    10261 gccctgacgg tggtcgtcac ccggcgcagg cccctgttgg ccatgaagtt ccgttacgaa
    10321 cccaacggca acatccgcga actgcaatgg aaagtgaccc gcca
//
LOCUS       DAORLF010000127        10326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3478_length_10326_cov_1.668581, whole genome shotgun sequence.
ACCESSION   DAORLF010000127 DAORLF010000000
VERSION     DAORLF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_10326_cov_1.668581"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>283
                     /locus_tag="PK198_11540"
     CDS             <1..>283
                     /locus_tag="PK198_11540"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF39417.1"
                     /translation="NDCFAKLGDRAWRDDNANGIQDPGEVGVPNVKVVVTGMAESTPY
                     SDTTFTDHTGMYMFLLPPGEYKLTFMLPPGSDLVPTVQNAGSDDAIDSDP"
     assembly_gap    284..399
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <400..1502
                     /locus_tag="PK198_11545"
     CDS             <400..1502
                     /locus_tag="PK198_11545"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39418.1"
                     /translation="NIGPAYQFLCGPGNIPAPLTNVTLPSGGTVTNQIEYMWMRTYTN
                     SPFDSGFWEIMPNSNSPSYQPGPLYQSAYFARCARRVECGPFIESNVVFVEVGTVSVA
                     NITGPEVVCLGDPATFSASGTAPGAVIQWSFGPGVSPNTATGSPVTVNFSSFGSFLVT
                     LTVTQNGCTASNFRYITVTDNPTFCMGNIQLDAEVTAAREVALEWSLQQGVPYTGFRV
                     ERSADGRVFQSVATVNAPWHTGSGMLRFAYDDASPKYGRNYYRVIMIGTDGLERASNV
                     AEAIIYGDSKLVLLYPNPVTDHVVLELFETFNGAVQVDVVTASGMVVKSEILQPNTPR
                     LEMDFSSYPAGVYFLRVRYGELELKRLKVLKL"
     gene            1749..>3186
                     /locus_tag="PK198_11550"
     CDS             1749..>3186
                     /locus_tag="PK198_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF39419.1"
                     /translation="MKINLRAVLTAIFTTASLAMASAQFILSFSVTEPTCFGLATGAV
                     TVTPIGGIGPYSYQWSTGAATQTISDIPAGTYGVTVTGANGVVSQNITVDQPTQVLAN
                     ISGDTCRLPVTLFAVGSGGTGPYTYTWDGMILGQTLTVTTPGTYCVTVVDTEWCGIVE
                     CVKISAGSINLNVQATNLICPNVNTGQVQATATGGLPPYTFQWSNGLQGPTISNLAAG
                     TYTVTVTDTRGCSVTATATVTSPPPLIAAITPTNPTCTGLNNGSVTATATGGTPPYTY
                     QWNNGALGPTRNNLGPGTYTVTVRDLNNCIVTQSVTLAPLSNLMVGAVPVPPTCPGVN
                     NGSVTANPSGGVPPYTYSWSIGGGGQVRNNLAPGTYSVTVTDALNCTAIATAVVPAAP
                     VFTIAMQSTNATTCGANNGTASVVVTQGAPPFTYQWSNGVTGTSIISNLSPGTYSVTV
                     TGVGGCTANGSVTITQPPLVFVNITASPR"
     assembly_gap    3187..3229
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3230..>3464
                     /locus_tag="PK198_11555"
     CDS             <3230..>3464
                     /locus_tag="PK198_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HRF39420.1"
                     /translation="GTPPFNFVWSTTGTGPAITGLPAGQYSVTVTDANGCQAFASANI
                     QQAPNPVVGVNANPIVCGLGNTGTATAFANNGTP"
     assembly_gap    3465..3633
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3634..>3909
                     /locus_tag="PK198_11560"
     CDS             <3634..>3909
                     /locus_tag="PK198_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF39421.1"
                     /translation="LCFGGNTGSAIASGSGGMPPYTYAWSNGVQGPVNANLAAGFYMV
                     TVTDFNGCSATQTAIISQPPALAVTINSSALQLCPGAADATLTAQPV"
     assembly_gap    3910..4007
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4008..6887
                     /locus_tag="PK198_11565"
     CDS             <4008..6887
                     /locus_tag="PK198_11565"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF39422.1"
                     /translation="TDMNGCTATASFTITQLQALQVTITGAEIVCGESNAGTAGVIVT
                     GGTGPYTFLWNTGANSEAVGGLGTGVYSVTVTDLNGCTSSAEIGIRVVDDFTASITPR
                     DLLCFGDNSGSALILAEGGNTPYTYLWSNGTTGPEAANLSAGVYSAIVTDANGCVLNV
                     QTTIQQPPLLQVSASAGNISCAGINNGTAIATATGGTAPYEYLWSNNQAGPTLSALAA
                     GTYTVTVFDVNNCTATTTVQITQPASFTVAINGTGPACAGASTGTATATATGGVGPYS
                     YLWSNGMTGSVRNGLPAGTYSVTATDANGCTASVSIVLSQPQAIVPAIQVLQQPCTGA
                     ATGALTASATGGTPPYSFTWSNGVSGAGLLNVQAGTYTVTVTDSRGCTATISQTLTAL
                     NSPSCSVEIANYVVQGNDGGLSVLVSGGTGPYSFLWSNGSTSPNPANLAPGAYSVTVT
                     DANGCTSTCTRVLPGASGVGDFVWRDDNQNGIQDPGEPGIPGVTVIVSGTQENDPYAD
                     TTVTNGNGIYFFNLPPGEYKLTFILPGGSDLTPTVANAGNNDNRDSDMDPVMFMTPYF
                     IVPPGVVDLSWDAGFVPPCINITNAGTIGPAYQFLCGPGNVPAPFVNVTLPSGGTDVV
                     PFEYMWMRSYVGGAFDNGFWEIMPNSNSPFYQSGPLSRSAYFARCARRDDCGPFLESN
                     VVFVEVGNISVANINGPEVVCLGQSATFTATGTAPGALIQWTFGPGGVVPAVAVGPSA
                     TVNFTSFGTFIVTLTVTQNGCTATNSRTITVTNSPIYCGNGLVINAEINSAREVQIEW
                     ELPENEPEHLYVIERSADGVGYDVLGELRNYHRITAGQKLFRFVDTRPKAGRNYYRIK
                     YVSTLGDTGYSNVEELILLGDSKLVLLYPNPVSGEAMLELFETFNGDVTLDIIAANGM
                     QLQRMVLPREVKRQPLDFAAYPAGVYFIRLRYGDVELKRLKVVKM"
     assembly_gap    7097..7137
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7138..>7475
                     /locus_tag="PK198_11570"
     CDS             <7138..>7475
                     /locus_tag="PK198_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39423.1"
                     /translation="PPGKPGEGKPRLSAAERAQKQTDRMKESLGLDEKQYKKVLEINQ
                     EHAKKVETQQLEAQKERERRQAAMKEMEAAHEKRMKEVLTEEQYVKYLVQKEQMKGRA
                     KARMNENRPQR"
     assembly_gap    7476..7568
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7587..>7960)
                     /locus_tag="PK198_11575"
     CDS             complement(7587..>7960)
                     /locus_tag="PK198_11575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF39424.1"
                     /translation="GDTIPAEFVDSLVHIKGALAAARMGDQANPTKASSGSQFYIVQG
                     RATDENTLNRLEAQKNTRYSSEQRNAYTTQGGTPFLDRDYTVFGYLIDGFDVLDRIAA
                     TPTQPGDRPREDVWMKVRVIK"
     assembly_gap    7961..7975
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7976..>8337)
                     /locus_tag="PK198_11580"
     CDS             complement(<7976..>8337)
                     /locus_tag="PK198_11580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF39425.1"
                     /translation="AYEWSFGDGKTSTEAEPVHRYSASGTYSVILKASDGKKHRMMEQ
                     KINIEAPDGCLVEIETSFGTMLARLSDATPKHRDNFLKLAEQGYYDNLLFHRVIEGFM
                     IQGGDPESKGAAPNTALGR"
     assembly_gap    8338..8387
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8512..>8773)
                     /locus_tag="PK198_11585"
     CDS             complement(8512..>8773)
                     /locus_tag="PK198_11585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF39426.1"
                     /translation="FYIVQGQRVDDNVLNGLEQQKGIRYSPEQRQLYREIGGAPFLDG
                     DYTVFGEVVEGLDVLDKIAAVPTAPGDRPLQDVKMKVKLVRK"
     assembly_gap    8774..8809
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8810..9162)
                     /locus_tag="PK198_11590"
     CDS             complement(<8810..9162)
                     /locus_tag="PK198_11590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF39427.1"
                     /translation="MKYILFIISAFILASCGDGNRYALIETEYGNMKVMLYNTTPKHR
                     DNFVKLAKEGYYDDLLFHRIIPNFMIQGGDPDSRNAAPGAMLGMGGPGYQIDAEIGSP
                     HLKGALAAARDQNPE"
     gene            complement(9386..9769)
                     /locus_tag="PK198_11595"
     CDS             complement(9386..9769)
                     /locus_tag="PK198_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39428.1"
                     /translation="MNHTMESWEFEFEWLRVRTFVKDAFQRDTLPDLNGMLFIIGMQE
                     LGRWKKSFSKEEKQDLMHIAVCRLLSYDGHYEFIGRDADGWPHWKPLKPVPPRPLEEQ
                     EQLLKEHVIRYIREQESENGTDISG"
     gene            complement(9778..>10093)
                     /locus_tag="PK198_11600"
     CDS             complement(9778..>10093)
                     /locus_tag="PK198_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HRF39429.1"
                     /translation="RNFVIRKMLMNHVNVHELEHYDDNYKSQLLEWCQKNGQTVTYKL
                     LARYKFEKRDRFKVAVMVDGQKVSTADDFNKKSAEQMASERALVTLGLLSEEDIEHHN
                     DD"
     assembly_gap    10094..10109
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10110..>10326)
                     /locus_tag="PK198_11605"
     CDS             complement(<10110..>10326)
                     /locus_tag="PK198_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRF39430.1"
                     /translation="YLFKKYPGSDEGFLTKMRSKIVKRQSLNLIADKMGLDEILAEYN
                     NTRLSRSMLGNALEALVGAIYMERGYVA"
BASE COUNT         2066 a         2645 c         2690 g         2248 t
ORIGIN      
        1 gaacgactgt tttgccaaac tcggcgaccg cgcctggcgc gacgacaacg ccaatggcat
       61 tcaagacccc ggagaggtgg gcgttcccaa tgtaaaagta gtagtgaccg gtatggccga
      121 aagtaccccc tactccgaca ccaccttcac cgatcacacg gggatgtaca tgttcctgtt
      181 gccgccgggt gagtacaaac tgacgttcat gttgccacct ggcagcgacc tggtgcctac
      241 agtgcaaaat gcaggcagcg acgacgccat agacagcgac ccgnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng taacatcggg cctgcctacc
      421 agttcctgtg tgggccgggc aatatacctg cgcccctgac gaatgtaacc ctgccctccg
      481 gcggcacggt caccaaccag atagagtaca tgtggatgcg gacttacacc aactctccat
      541 tcgatagcgg tttctgggag atcatgccca acagcaattc gcccagctac cagccggggc
      601 cgctgtatca aagcgcctat tttgctcgtt gtgcgcgccg ggtagagtgc gggccgttta
      661 tagagtccaa cgtcgtgttt gtggaagtag gcacggtatc tgtagccaac atcacggggc
      721 cggaagtagt atgcctcggc gacccggcca ctttctctgc ttcgggtaca gctccggggg
      781 cggtgattca atggagcttt ggtccgggcg tatctccgaa cactgctacg ggttcgccgg
      841 taacggtcaa tttctcctcc ttcggctcgt ttttagtcac tttgacggtg acgcaaaacg
      901 gctgtacggc ctccaacttc cgctacatta ccgtcactga taacccgact ttctgcatgg
      961 gcaatattca gttggatgca gaggtgaccg ccgccagaga agttgcattg gaatggtcgc
     1021 tccaacaggg agtgccttac acgggtttcc gcgtggagcg ctctgccgac ggacgggtat
     1081 ttcagtctgt tgcgacagtg aacgctccct ggcataccgg ttcaggtatg ttgcgctttg
     1141 cttacgatga cgcctcgccg aagtacgggc gcaattacta ccgcgtcatt atgataggaa
     1201 cagacgggct ggagcgggca tccaacgtag cagaggccat catttacggc gattccaagt
     1261 tggtcttgtt gtatcccaac ccggttacgg atcatgtcgt attggaattg tttgaaacct
     1321 tcaacggcgc tgtgcaggtg gacgtagtaa cggcgagcgg catggtggtc aaatcagaga
     1381 tattgcaacc caatactccg cgattggaaa tggacttctc ttcgtatccg gcaggcgttt
     1441 acttcctgcg ggttcgatat ggtgagttgg agttgaaacg attgaaagtg ttgaagttat
     1501 aaaatctaat tggttggatg tcggctcaaa ttctcaaacg tcggcgtcca accctctttg
     1561 ttctctgccc ttcgcccctc tgaaaaatct cccttaaaaa aacaaccgca gcaggttttg
     1621 cgcctgccgc caattggtaa ttattaccct gatctgttgt attgacagca gcgcgaggta
     1681 tttgtatgaa agttttttca ctctgtaaat caaatctcat gcgaacgagt ttacaccaaa
     1741 cggccacaat gaaaataaac cttcgggctg tgttaacggc catttttacg acagcgagct
     1801 tagctatggc ttcggcccag tttattctta gcttttccgt tacggagcct acttgcttcg
     1861 ggcttgccac cggggccgtt accgtgacgc ccatcggcgg tatcgggccg tactcttacc
     1921 aatggagtac gggcgctgca actcagacca tctctgatat tccggcgggt acttacggtg
     1981 tcaccgtcac cggggccaac ggggtggtat cgcaaaacat caccgtggac cagcccacgc
     2041 aggtgctggc caacatttcg ggcgatacct gccgcctgcc ggtaacatta tttgctgtag
     2101 gatcgggtgg gaccggccct tatacctaca cctgggacgg catgattctc ggtcagacgc
     2161 tcacggtgac aacgccgggc acttattgcg ttacggtagt tgatacagag tggtgcggca
     2221 tagtggaatg tgtaaaaatc tcggcaggat ctatcaatct aaacgtgcag gccaccaatc
     2281 tgatttgccc caatgtgaat acggggcagg tacaggcaac tgctacgggc ggtttgccgc
     2341 cttatacttt tcagtggagc aatggtttgc aggggccgac catcagcaat ctagctgccg
     2401 gaacctatac tgtaacggtg accgatacgc ggggttgctc ggtgacggcc acagctaccg
     2461 tgacttcgcc gccgccactg atcgctgcca tcaccccaac gaaccctacc tgtaccggcc
     2521 tcaacaacgg cagcgtcacg gctacggcca ccggtggaac tcccccctat acttaccaat
     2581 ggaacaacgg tgcattaggc ccgacgcgca acaatctggg gccgggcact tacaccgtga
     2641 cggttcggga tttgaataac tgtattgtca cacaatcggt tacgctggcg cctttgtcca
     2701 acctgatggt gggcgcagta ccggttccgc ctacctgtcc gggagtgaac aacggcagcg
     2761 tgacggccaa tccatcgggt ggcgtgccgc catacaccta ctcgtggagc ataggcggcg
     2821 gcgggcaagt gcgcaacaac ctcgcgcccg gcacttacag cgtaacggta acagacgcgc
     2881 tgaactgtac ggctattgct acggctgttg ttccggcagc gccagtattc accattgcca
     2941 tgcagtccac taatgccact acctgcggag ccaacaacgg cacggcttcg gtagtagtca
     3001 cgcagggcgc gccgccgttt acctaccaat ggagcaacgg cgtgaccggc acttccatca
     3061 tttccaattt gtcgccgggt acttattctg taacggtgac gggcgtcggc ggatgtacgg
     3121 ccaatggttc ggtcacaatc acacagcctc cgctggtctt tgtcaacatt accgcttcgc
     3181 ctcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcacgcctcc
     3241 cttcaatttc gtgtggagta cgacggggac gggtcctgct attacaggtt tgcctgccgg
     3301 ccaatactcg gtgacggtaa cggatgccaa tggttgtcag gcatttgcaa gcgccaatat
     3361 ccaacaggct ccgaacccgg tggtgggcgt aaacgccaat cccattgtgt gtggcttggg
     3421 caataccggc acggcgacgg ctttcgccaa taacggaacc cctcnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgctgtg tttcggaggc aatactggta
     3661 gcgccattgc ttcgggcagc ggcggcatgc caccttacac ttatgcgtgg agcaatggcg
     3721 tgcaaggacc ggtgaatgcc aatctggcgg cgggttttta catggttacg gttacggatt
     3781 tcaacggctg ctcagccact caaactgcca tcatttcgca gccgccggca ttggcggtga
     3841 ccatcaactc gagcgctttg caactctgcc ccggagcagc ggatgcgacc cttacggcgc
     3901 agcccgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc gatatgaatg
     4021 gctgtacggc tacggcatct tttacgatca cacagttaca ggcattgcaa gtgaccatta
     4081 ccggcgcaga aatcgtttgt ggggagtcga atgcaggcac ggcgggggtc atcgttacgg
     4141 gcggtaccgg gccttacact ttcttgtgga ataccggcgc caactccgaa gcagtgggcg
     4201 gcttgggtac gggcgtttac tctgtgaccg taaccgacct gaatggatgc acatcaagcg
     4261 cggagatcgg cattcgtgtg gtggatgatt ttacggcaag tattaccccg cgcgatctgc
     4321 tctgctttgg cgacaatagc ggcagcgccc tcattttagc tgaaggcggc aacacgccct
     4381 atacctacct gtggagcaac ggcacaaccg gcccggaggc tgccaatctg agtgccggag
     4441 tatattcggc cattgtaacc gatgccaacg gctgtgtttt gaatgtacaa actaccatcc
     4501 aacaaccgcc gctcttgcag gtttccgcct cagcgggcaa tatatcctgc gccggcatca
     4561 acaacggcac ggccatcgcc acggctaccg gtggcaccgc gccttatgaa tatttgtgga
     4621 gcaacaatca ggcaggcccc accttgtcgg ctctggcagc ggggacatac acggtcactg
     4681 tttttgatgt aaataattgt accgctacta ccaccgtaca gattacgcag ccggcatcgt
     4741 tcacagtggc catcaacgga accggccctg cctgcgcggg tgcatctacc ggcacagcta
     4801 cggctacggc taccggtggg gtggggccgt attcttacct ttggagcaac ggcatgaccg
     4861 gctcggtacg aaacggactg cctgcgggta cttactccgt cactgccacg gatgccaatg
     4921 gctgcacggc ctcggtctct atcgtactgt ctcagccaca ggctattgtg cctgctattc
     4981 aggtcttgca acagccctgt accggtgcgg ctaccggcgc attgacagca tccgctactg
     5041 gcggaacgcc cccatacagt ttcacctgga gcaatggcgt atcgggcgcc ggtttgctca
     5101 atgttcaggc aggcacttac accgtgaccg tcaccgattc gagaggctgt accgctacca
     5161 tctcacaaac cctgaccgca ctgaacagcc cctcttgcag cgtggaaatt gccaactatg
     5221 tagtgcaggg caatgacggc ggactgagcg tattggtcag cggcggcacg gggccttata
     5281 gtttcctctg gagcaacggt tctacatcgc ctaacccggc caacctcgca ccgggcgctt
     5341 attcggtaac ggtgacggat gccaacggct gcacctccac ctgtacccgc gtgctgcccg
     5401 gagcttccgg cgtaggcgat tttgtgtggc gcgacgataa tcaaaacggc atacaagacc
     5461 ccggcgaacc cggcattccc ggcgtaacgg tgattgtttc cggtacgcag gaaaatgacc
     5521 cctacgccga taccacggtg accaatggca acggcatcta tttcttcaat ctgcctccgg
     5581 gcgagtacaa actcactttc atcctgccgg gcggaagcga cctgacgccc actgtggcca
     5641 acgccggtaa taacgacaac cgcgacagcg atatggaccc ggtgatgttt atgacgccat
     5701 atttcatagt gcctccgggg gtggtggatt tgagctggga cgcgggtttt gtgccgcctt
     5761 gcatcaatat taccaatgca ggtacgatag gcccggctta tcaattcctt tgcgggccgg
     5821 gcaatgtgcc tgcgccgttt gtgaacgtaa ccctgccttc cggcggcaca gatgtggtgc
     5881 cgttcgagta tatgtggatg cgcagctatg tcggcggggc gttcgataac ggtttttggg
     5941 agatcatgcc caacagcaac tcacccttct atcagtcggg gccgctttcc cgcagcgctt
     6001 actttgctcg ctgtgcccgc cgcgatgatt gcgggccgtt cttagaatcc aatgtggtgt
     6061 ttgtagaagt gggcaacatt tctgtggcca acatcaacgg tccggaggtc gtatgcctgg
     6121 ggcaatcggc tacatttacg gctaccggca cagcgccggg cgccttgatt cagtggacct
     6181 ttggacctgg gggcgtagtg cctgcggtgg ctgtggggcc gtcggctacg gtcaatttta
     6241 cttcattcgg cacctttatt gtcacgctta ccgttacaca aaacggctgc acggctacca
     6301 actcgcgtac cataaccgtc accaatagcc ccatctattg cggcaatggc ttggttatca
     6361 acgccgaaat caactcggcc cgcgaggtgc aaatcgaatg ggaactgccc gaaaacgagc
     6421 cggagcattt gtacgtcatt gagcgttcgg cagacggcgt tgggtatgat gtgctgggag
     6481 aactccgaaa ttaccacaga atcaccgccg ggcaaaagct gttccggttt gtggacacac
     6541 ggcccaaagc cggcagaaat tactatcgca tcaagtatgt ctccactttg ggcgataccg
     6601 gctactccaa tgtggaggag ctcattttgt tgggcgattc caaattggtg ctgctgtatc
     6661 ccaacccggt gagcggcgag gccatgctgg aattgtttga aaccttcaac ggagacgtga
     6721 ctttggacat cattgcggcc aacggtatgc aattgcaacg catggtactg ccccgcgagg
     6781 tgaaacggca accgcttgat tttgcagcgt atccggcggg tgtgtatttc atccgtttgc
     6841 gttacggcga tgtggagttg aagcgcctga aggtggtgaa gatgtaaggc gttaacatat
     6901 ctattgacgg ttttcatggg cttctggcca atagccaata gctgttagcg tgttattcaa
     6961 cagagttaac actaattaac gaaggcgggt taatcctgag tggaatgttt cggtcttatg
     7021 ctcgaatcaa ataactaatt gacatgagaa agatgaagca atccattcag gtcgccctgc
     7081 tggggatggc attattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     7141 cccggcaaac ccggcgaggg caagccacgc ctcagcgccg ccgaacgcgc acagaaacaa
     7201 accgaccgca tgaaggaatc tttgggtctc gatgaaaagc agtacaaaaa ggtgctggaa
     7261 atcaatcagg aacacgccaa aaaagtagaa actcagcagt tggaagccca gaaagagcgc
     7321 gagcgtcgcc aggcagccat gaaggagatg gaggctgcac acgaaaagcg gatgaaggaa
     7381 gtcctcaccg aagagcagta tgtgaaatac ctcgttcaga aggagcaaat gaaaggacgg
     7441 gccaaagcgc gcatgaatga aaatcgcccg cagcgnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnag ttgggatggc cccctcttac tttatcaccc gcaccttcat ccacacatcc
     7621 tcgcgggggc ggtcgccggg ctgcgtaggc gtagcggcga tgcggtcgag tacatcgaag
     7681 ccgtcaatga ggtagccgaa aacggtgtag tctctgtcta agaacggcgt gccgccctgc
     7741 gtcgtgtagg cattgcgttg ctcggaagag tagcgcgtat ttttttgtgc ctctaagcgg
     7801 ttgagggtat tttcatcggt ggcacggccc tgcacgatgt agaactgcga gccggaagaa
     7861 gccttggtcg ggttggcctg atctcccatg cgggcagccg ccagcgcgcc tttgatgtgt
     7921 accagcgagt ccacaaactc tgccggaatg gtatcgcccg nnnnnnnnnn nnnnncggcc
     7981 taatgctgta ttgggtgcag cacctttaga ttcggggtcg ccgccctgaa tcataaaacc
     8041 ctcaatgacc ctgtgaaaca acaagttgtc atagtagccc tgttcggcga gtttgagaaa
     8101 attatcgcgg tgcttgggcg tggcgtcgga gaggcgagcc agcatggtgc cgaaagaagt
     8161 ttcaatttct acgaggcagc cgtcgggcgc ttcaatgttg attttttgtt ccatcatgcg
     8221 atgttttttg ccgtcggagg ctttgagaat gactgaataa gtgccggaag cggagtagcg
     8281 gtgtacaggc tcggcctcgg tagaggtttt gccgtcgccg aagctccact cgtaggcnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg gaggaaggcg
     8401 ctttgccgcc tccgtcgtaa gaaaactgcg cgatgggccg cgagcaaccg gatgccagcg
     8461 cgatggccgc cactgccgcg aaaagcaatg ttttagaaga catgttgttt atcattttct
     8521 gaccaatttc actttcattt ttacatcctg caaggggcgg tcgccgggtg cggtgggcac
     8581 ggcggctatt ttatcgagca catccaagcc ttctaccacc tcgccaaaaa cagtgtaatc
     8641 gccatctaaa aaaggtgcgc cgccgatttc gcggtacagt tggcgttgtt cggggctgta
     8701 ccggatgcct ttttgctgct ccaatccatt gagcacgttg tcgtcaacgc gctgaccttg
     8761 tacaatgtaa aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tttcaggatt
     8821 ttggtcgcgg gcggcagcca gcgcgccttt caaatggggc gagccgatct ctgcatcaat
     8881 ctgatagccg gggccgccca tgcccaacat agcgcccgga gcggcattgc gggagtcggg
     8941 gtcgccgccc tgaatcataa agttgggaat gatgcgatgg aagagcaaat catcatagta
     9001 gccttctttg gccaatttga cgaagttgtc gcggtgcttg ggcgtcgtgt tgtacagcat
     9061 cactttcatg ttgccgtact cggtttcgat gagggcgtag cgattgccgt cgccgcaaga
     9121 cgccagtatg aatgcgctga taatgaagag tatgtatttc atggttttgt gatttaatac
     9181 ctaaaattga gaggctttta ttggggcgcc cccgccacaa gtactgtcat tcgggcgggc
     9241 gccgtgcctg ccggcagggg ccatccactg catggcggct acttcgcaga gctgttagct
     9301 ttatccggct attgcagtgc taaagctaac agctctatcc tcgacacgcc gccattccgt
     9361 ttctgtccct ggcgcaacat ttccctcagc ccgatatgtc tgttccattt tcagactctt
     9421 gctctctgat gtaccgaatg acgtgctctt tgagcaactg ctcctgctcc tccagtggtc
     9481 ttggcggcac cggtttcagc ggtttccaat gcggccagcc gtcggcatcc cgaccaatga
     9541 actcgtagtg cccgtcgtag ctgagcagtc ggcaaacggc aatgtgcatc aggtcttgtt
     9601 tttcctcttt ggaaaagctc tttttccagc ggcccaactc ctgcatacca atgatgaaca
     9661 acatgccgtt gaggtcggga agtgtgtcgc gttgaaaggc atccttgacg aaggtgcgca
     9721 cgcgcaacca ctcaaattca aattcccagc tttccatagt gtggttcatg cgcgaggtca
     9781 atcgtcgttg tggtgttcga tgtcttcttc gctgagcagc cccaaagtga ccaaggcgcg
     9841 ttcggatgcc atctgttcgg cgcttttttt gttaaaatcg tcggcagtag agaccttttg
     9901 cccgtccacc ataacggcca ctttgaagcg gtcgcgtttt tcaaatttgt atcgggccag
     9961 cagtttgtag gtcacggttt ggccgttttt ctggcaccat tccagcagtt ggcttttgta
    10021 attgtcgtca taatgctcca attcatgcac attcacatgg ttcataagca tttttcggat
    10081 cacaaaattt cggnnnnnnn nnnnnnnnng gccacatagc ctcgttccat gtaaatggcg
    10141 cccaccaatg cttcgagggc attgccgagc atggaacggc tgaggcgggt attgttgtac
    10201 tcggccagta tctcatccaa acccattttg tcggctatca gattgagcga ctggcgtttg
    10261 acgattttgg agcgcatctt ggtgaggaag ccctcatcgc tgccggggta ttttttaaac
    10321 agatat
//
LOCUS       DAORLF010000128        10285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3497_length_10285_cov_2.140078, whole genome shotgun sequence.
ACCESSION   DAORLF010000128 DAORLF010000000
VERSION     DAORLF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3497_length_10285_cov_2.140078"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..643
                     /locus_tag="PK198_11610"
     CDS             149..643
                     /locus_tag="PK198_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39431.1"
                     /translation="MSQTTVTFAQFLAKFPEIELPVVLNDEAHHTFSTENSPLAEAMI
                     AQYILPAEPEEADEMTEFVPCFKLPKTEGFHAVVYWRAGLMNYQYVLVTFAKKGQLID
                     RRVIAGTFVNGQMITQSVATINEENEIFVASGQSDAQDETYDPASSTAYELELLPDGL
                     IVNN"
     gene            665..2845
                     /gene="rnr"
                     /locus_tag="PK198_11615"
     CDS             665..2845
                     /gene="rnr"
                     /locus_tag="PK198_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRF39432.1"
                     /translation="MTKQRKPREKPAGRGKTSQGRKLSAAQLQQAVLQLFQNNPKKRF
                     NPKQVIQHLRIDNNKDSVQHALHTLVEARLLAPLEDFKFKLRSEGPPTSKIGSYEGYI
                     DMTRTGSAYVTCDKVEEDVHVAARYMNGAMHGDRVRLNVWKPRGRQRPEGEVLEVLER
                     ASTHFVGTIWLHHRYAVVSPEMKVPLDIYVELANTKGAKDGDKVVVKITKYDTGRFTT
                     MEGVVTTVLGQAGSHDIEMQAILINNGFDLEFSETALEEAAGISESIETEEIARRRDM
                     RDTFTITIDPFNAKDFDDALSLRYLDDGLCEVGVHIADVTHYLKPDGALDKDAYRRST
                     SVYLVDRVLPMLPEKLSNELCSLRPNEDKLTFSAVFVFDKNSKIIERWFGKTVIHSDR
                     RFTYEEVQEILDAGQGEHVKELKHLNKIAEKLRKDRFKKGSINFETPEVQFRLAPDGT
                     PIEAFVKERKDAHLLIEDFMLLANREVATFIHEKGKGSEIPFVYRIHDEPNPEKLEEF
                     ARFAREFGFDMDLGSPKGISSSLNRLVEAARHEPALKLLEQPAIRTMAKAVYSTGNIG
                     HYGLAFQYYSHFTSPIRRYSDVLAHRILEQNLLPDVYYRVKKEKLEEQCKHISMQERK
                     AMEAERESVKYKQAEYMEKHLGQEFDGVISGMSERGIFVTLTETLCEGMVSFDSMGEP
                     FRELNSKLRVKGMYSGRELKTGDAIRVKVVRTDLSRRVIDMELV"
     gene            2979..5219
                     /locus_tag="PK198_11620"
     CDS             2979..5219
                     /locus_tag="PK198_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRF39433.1"
                     /translation="MALKLGVNDERIPKLLGVICWFLAFYLFIAFFSYLFTWKTDQDE
                     VLRFSWSLLTADVSMSNWLGRLGAIISNMFFYWGLGLPSFGFVYLLMAGGRSLMKRLD
                     ASVWIDKLRITSVLMLLFAVFLEFIFGNAAFPWGGAIGESIATWLQNLLGVVGLGTLF
                     LLVIIGVITWVFNPNYNDMTFRSAVESVTEYIRDFFTGRLYRRQRAAEQAAANAAAEE
                     KAQPRPRPLPAGEEESQLSLDLDQRLRPTSPSLRPEGAELEIQTPIAPEPEPIATSAA
                     PPPMQPSDNIAGISQENVDHSEPYDPTLELSSYENPVLSLLEDYSDQKVEVDRAELEA
                     NKDQIIETLLNYKIEITKIRATIGPTVTLYEIVPAPGVRISRIKNLEDDIALSLAALG
                     IRIIAPIPGKGTIGIEVPNKNKQVVKEVLQHEKFRRAKMDLPIALGKTISNEIVVTDL
                     AKMPHLLMAGATGQGKSVGINAVLMSLLYKKHPSQVKLVLIDPKKVELSPYGKLENHY
                     LAILPGQEEPIVTETNKVIHTLNSLCIEMDTRYNLLKKAGARNIREYNEKFVQRQLNP
                     QKGHKFMPFIVLVIDEFADLIMTAGKEVELPLGRLAQLARAIGIHIIIATQRPSVNII
                     TGVIKANFPARVAFKVSAKVDSRTILDASGADQLIGRGDMLLSVGGDMIRVQCAFVDT
                     PEVERVVEFINQQPGYAEPYLLPEFHGEEGEGLAGSNGPLRYSDLDEMFEDAASSSKR
                     STALPP"
     gene            5216..5383
                     /locus_tag="PK198_11625"
     CDS             5216..5383
                     /locus_tag="PK198_11625"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRF39434.1"
                     /translation="MIQRRLKLGYNRAGRLMDQLESAGIVGAADGSKAREVLIYDEME
                     LNRFLEDLKRR"
     gene            5398..7074
                     /locus_tag="PK198_11630"
     CDS             5398..7074
                     /locus_tag="PK198_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRF39435.1"
                     /translation="MTRIIFTLTFSLFCGLLFSQTADELLRDLQQATTAKEKMLINFQ
                     IAETYMRGGDREKAIDFARTAHQSSIEQSNNGMAARSAYLIGDAYERLRNDRNAETWY
                     KTCLNFAKTAGDFDLIVQSVKKRSKIATKDGNYRRAYEINQEAFDFFSGKGTSISDLE
                     GKYENQKVILDREKRVLVEEKNRLEREIASMAAERDQLSTDKTHLESRQQELVQQKAK
                     VEEQISEKEQALVSVAEEKRKVEEVAQQKEAALKNLSREKLIQEAALNAAKKESAEAE
                     LAVQQEKSLREQEKSLREKTLLQALLGGAIGALILFFMTLLYLSSRRAKSSLQRKNKL
                     IEMERERSDELLFNILPKSIGEELKEFGKARARKFEQATVLFTDFKNFTKISETLSPE
                     DLVAEIDRCFKGFDFIISQYPDIEKIKTIGDAYMCASGLNEGGSSPDNLLRAALEMQE
                     FLDEQKVERQRLGKPYFEARIGLHTGPVVAGVVGVNKFAYDIWGDTVNIAARMEAQCE
                     EGRINISETTYNLVRYSFECTYRGKVEAKNKGAIDMYYVERELAGSGVYS"
     gene            7601..7674
                     /locus_tag="PK198_11635"
     tRNA            7601..7674
                     /locus_tag="PK198_11635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7635..7637,aa:Leu,seq:cag)
     gene            7684..7756
                     /locus_tag="PK198_11640"
     tRNA            7684..7756
                     /locus_tag="PK198_11640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7717..7719,aa:Asp,seq:gtc)
     gene            7765..7835
                     /locus_tag="PK198_11645"
     tRNA            7765..7835
                     /locus_tag="PK198_11645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Arg,seq:acg)
     gene            7843..7913
                     /locus_tag="PK198_11650"
     tRNA            7843..7913
                     /locus_tag="PK198_11650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7875..7877,aa:Gly,seq:gcc)
     gene            7922..7997
                     /locus_tag="PK198_11655"
     tRNA            7922..7997
                     /locus_tag="PK198_11655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7955..7957,aa:Met,seq:cat)
     gene            8001..8073
                     /locus_tag="PK198_11660"
     tRNA            8001..8073
                     /locus_tag="PK198_11660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8034..8036,aa:Lys,seq:ttt)
     gene            8083..8153
                     /locus_tag="PK198_11665"
     tRNA            8083..8153
                     /locus_tag="PK198_11665"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8115..8117,aa:Phe,seq:gaa)
     gene            8170..8242
                     /locus_tag="PK198_11670"
     tRNA            8170..8242
                     /locus_tag="PK198_11670"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8203..8205,aa:Cys,seq:gca)
     gene            8427..8502
                     /locus_tag="PK198_11675"
     tRNA            8427..8502
                     /locus_tag="PK198_11675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8460..8462,aa:Pro,seq:tgg)
     assembly_gap    8591..8651
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8677..>9638
                     /locus_tag="PK198_11680"
     CDS             8677..>9638
                     /locus_tag="PK198_11680"
                     /inference="COORDINATES: protein motif:HMM:NF017541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HRF39436.1"
                     /translation="MARFNFSVGKKNKTVNRAGGNAFVQSPEMQLASLLLTSFAQDQF
                     YRDTKQTFSDLSALLAKVNPEFAAKAAVYARTEFGMRSITHILAAELGMYASGKAWGK
                     AFYEKIVLRPDDMTEIASLMLAENGAKLPNALRKGFAKAFDKFDGYQLAKYRAEGKAV
                     KLVDLVNLVRPVPVARNAEALRQLTAGELRNTDTWEAKLTKAGATAETDAEKAELKAE
                     AWAELLRTRKIGYFALLRNLRNIAEQAPELVPTACELLTDERLIRKSLVLPFRFSTAL
                     EALETANIADKRSLVKALNRAVDLSLANVPVLPGRTLVVLDDSGS"
     assembly_gap    9639..9650
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9651..10116
                     /locus_tag="PK198_11685"
     CDS             <9651..10116
                     /locus_tag="PK198_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39437.1"
                     /translation="PIQVGSMFAAVLYRSNDADLMRFSDSASYLRQNPDNPVMTIAEA
                     LVKNAYMAGTNFNAIFEKAKRAYDRIIILSDMQGWVGGGAPTKAFADYKARYNADPFV
                     YSFDLQGYGSLQFPESKVFAIAGFSEKVLELMGQLERDPQALIRAIEAVELA"
BASE COUNT         2598 a         2840 c         2624 g         2150 t
ORIGIN      
        1 gtgcgcagga accggaactc tgggactatg ccgacggcgt ggatacgatg cagtttttgt
       61 tgttgctggg gtgaggaatc ttttccctcc tttgcgctgt tatgtaatct gaaaaaagca
      121 gccccttttg cattacgaac caaccaacat gagtcaaaca accgtaactt ttgcgcaatt
      181 cttagccaaa tttcccgaaa tcgaattgcc tgtcgtgctc aacgacgaag cgcaccacac
      241 cttcagcacc gaaaacagcc cgttggccga ggccatgatc gcgcagtaca tcctgcccgc
      301 tgaaccggaa gaagctgacg aaatgaccga gtttgtacct tgcttcaaac tgcccaaaac
      361 agaaggattt catgccgtcg tttactggcg ggccggcctg atgaactacc agtatgtgct
      421 ggtcaccttt gctaaaaaag gccaactgat agaccgccgg gtaatagccg gaacttttgt
      481 gaacggccaa atgattacgc agtcagtagc caccatcaat gaagaaaacg aaatttttgt
      541 ggcctccggg cagtccgatg ctcaagacga aacctacgac cccgcgtcca gtaccgctta
      601 tgaattggaa ttgctgcccg acggcctgat tgtcaataat taaatgtatt cacacaactt
      661 acccatgacc aaacaacgca aaccgcgcga aaaacctgcc ggacgaggca aaacctcaca
      721 gggcagaaaa ctcagcgcag cccaactaca acaagctgtt ttacaactgt ttcagaacaa
      781 tcctaaaaag cgattcaacc ctaaacaagt cattcagcac cttcggatag acaacaacaa
      841 agactctgtg cagcacgccc tccacacatt ggtggaagcc cgcctgctgg cgccgctcga
      901 agattttaag ttcaaactgc ggtctgaagg acccccgacc tccaaaatcg gctcttacga
      961 gggctacatt gacatgaccc gcaccggctc ggcctacgtc acctgcgaca aagtggaaga
     1021 ggacgttcac gttgctgcac ggtacatgaa cggcgccatg cacggcgacc gcgtgcgcct
     1081 caacgtgtgg aaaccccgcg gccgccaacg cccggaagga gaggtgctgg aagtactcga
     1141 acgcgcctcc acccattttg tgggcaccat ctggctgcac catcgctatg ctgtggtatc
     1201 gcccgaaatg aaggtgccgc tcgatattta tgtggaactc gccaacacca aaggcgccaa
     1261 agacggcgat aaagtggtgg taaaaataac gaagtacgac accggccgct tcaccacgat
     1321 ggaaggcgta gtgaccaccg tactcggcca ggcaggcagc cacgatattg aaatgcaggc
     1381 catcctcatc aacaatggtt ttgatctgga attttcggaa accgccctcg aagaagccgc
     1441 cggcatttca gaaagcattg aaacagaaga aattgcccgc cgccgggaca tgcgcgacac
     1501 cttcaccatc accattgacc ccttcaacgc caaagacttt gacgatgcac tctcgctgcg
     1561 ctacttagac gacggcctct gcgaagtggg cgtgcatatt gccgatgtga cgcactacct
     1621 caagcccgac ggcgccttgg ataaagacgc ctaccgccgt tctacctcgg tgtatttggt
     1681 ggatcgcgtg ctgcccatgc tgcccgaaaa gctctccaac gaactgtgct cgctgcggcc
     1741 caacgaagac aaactcacgt tttcggccgt ttttgtattt gataaaaaca gcaaaatcat
     1801 agagcgctgg tttggcaaaa ccgtcattca ttccgatcgc cgcttcactt atgaagaagt
     1861 gcaggaaatt ttggatgcag gccagggcga gcatgtaaag gagttgaaac acctgaacaa
     1921 aatagccgaa aagctgcgca aagaccgctt caaaaagggt tccatcaatt ttgaaacccc
     1981 ggaggtacag ttccgcctgg cccccgacgg cacgcccatc gaagcctttg taaaagagcg
     2041 caaagacgca cacctgctca tagaagattt catgttgctg gccaatcgcg aagtggccac
     2101 gttcattcat gaaaaaggca aaggcagcga aatccccttc gtgtaccgca tccacgacga
     2161 gccgaatcct gaaaagctgg aagaatttgc ccgtttcgcc cgcgagttcg gtttcgatat
     2221 ggatttgggt tcgcccaaag gtatttccag ttcgctcaat cgcttggtag aagcggctcg
     2281 tcacgaaccc gcgctcaagc tcctcgaaca gcccgctatc cgcaccatgg ccaaagccgt
     2341 gtactccacc ggcaacatag gccactacgg gctggcattc cagtattatt cgcacttcac
     2401 ctcgcccata cgccgctact ccgacgtgct ggcacacagg attttagagc aaaacctgct
     2461 gcccgatgtc tattaccgcg tcaaaaaaga gaagctcgaa gagcagtgca agcacatctc
     2521 catgcaggaa cgcaaggcca tggaggccga gcgggaatcg gtgaagtaca agcaggccga
     2581 atacatggaa aaacacctgg ggcaggagtt cgacggcgtc atttcgggca tgtcggagcg
     2641 gggcattttc gttaccctca ccgaaacgct gtgcgaaggc atggtgagtt tcgattcgat
     2701 gggcgagccg ttccgcgaac tcaatagtaa gttgagagtc aagggaatgt actctggtcg
     2761 cgagctgaaa accggcgatg cgatccgcgt aaaagtggta cgtaccgacc tcagccggcg
     2821 ggtgatagat atggaactgg tatagggcaa aaacacttcc ccgccaaaat actaccatac
     2881 ctccgcgata ttgtctattt ttgcccatcg tctgcaccaa caacatcgtg cttacgacct
     2941 tttagtacat ggcgacgaaa ccgcgaaata aaaaccagtt ggcgctcaaa cttggcgtaa
     3001 atgacgagcg catcccgaag ctgttaggtg taatatgctg gtttctggca ttttacctct
     3061 tcatagcctt cttttcctat ctcttcacct ggaagactga tcaggacgaa gtgctccgtt
     3121 tttcatggag tttgctgacc gcagacgtga gtatgtccaa ttggttgggc cgtttgggag
     3181 ccattatttc caacatgttt ttttattggg gactggggct tccgtccttt ggctttgtgt
     3241 atctgctcat ggccggcggc aggtctttga tgaagcgctt ggacgcttct gtgtggatag
     3301 ataaactacg catcacgtct gttttgatgt tgttgtttgc ggtattttta gagtttatct
     3361 tcggcaatgc agcctttccc tggggcggcg ccattggcga atccattgct acctggctgc
     3421 aaaatctgtt gggagtggta ggtctcggca cgctgtttct gctcgtcatc atcggcgtca
     3481 tcacctgggt attcaatccc aactacaacg acatgacctt ccgcagcgcg gtggagagcg
     3541 tgacggaata catccgcgat tttttcaccg gcaggctcta ccggcgtcaa agggcagccg
     3601 aacaagccgc cgccaatgct gccgctgaag aaaaagcaca gccacgcccg cggccattac
     3661 cggccgggga agaagaatcc caactctcgc tcgatcttga tcagcgtctg cgtcctacct
     3721 ccccctcgct tcgtccggaa ggcgccgagt tggaaatcca aacgcccata gcgcctgaac
     3781 ctgagccgat tgccaccagt gctgcgccgc cgccgatgca accatccgac aacatcgccg
     3841 gcatttcgca ggaaaatgtt gaccacagcg agccgtatga tcccacgctg gagttgtctt
     3901 cgtacgaaaa tccggtgttg tctttgctgg aagattattc cgatcaaaaa gtagaagtgg
     3961 accgcgccga attggaggcc aacaaagacc agatcatcga aaccctgctc aattacaaaa
     4021 tcgagatcac caaaatacgc gccaccatcg ggccaacagt cacgctgtac gaaattgtgc
     4081 cggcgcccgg cgtgcgcatc agtcgcatca aaaacctcga agacgacatc gcgctgagcc
     4141 tggccgcgtt gggcatccgc atcattgcgc ccatccccgg caagggcacc atcggtattg
     4201 aagtgcccaa caaaaacaag caggttgtga aggaggtgct gcagcacgaa aaattccgcc
     4261 gcgccaaaat ggacctgccc atcgccttgg gcaaaaccat ctccaacgaa atcgtagtga
     4321 ccgaccttgc caagatgcct cacctgctca tggccggtgc tacggggcag ggtaaatcgg
     4381 tgggtatcaa cgcagtactc atgtcgctgt tgtacaaaaa gcacccttcg caggtcaagc
     4441 tcgtactcat tgaccccaaa aaagtggagc tttcgcctta cggcaaactc gaaaaccact
     4501 atttggccat tctgcccggc caggaagaac ccattgtgac cgaaaccaac aaggtcatcc
     4561 atacgctcaa ctccctgtgc atcgaaatgg acacgcgcta caacctgctc aaaaaagccg
     4621 gcgcgcgcaa catccgcgag tacaatgaga agtttgtgca acgccagctc aacccgcaaa
     4681 agggccacaa attcatgcct ttcatcgtgc tggtgataga cgaatttgcc gacctcatca
     4741 tgaccgccgg caaggaagta gaactgcccc tgggccgcct tgctcaattg gcccgcgcca
     4801 tcggcatcca catcatcatt gctacacagc gcccctcggt caacatcatc acgggcgtta
     4861 tcaaggccaa cttcccggcc cgcgtcgcgt tcaaagtatc ggccaaagtg gactcccgca
     4921 ccatcctcga cgccagcggc gccgaccagc tcatcggtcg gggcgacatg ctgctgtccg
     4981 taggcggcga catgattcgc gtgcagtgcg cctttgtgga cacgcccgaa gtggagcgcg
     5041 tggtagagtt catcaatcag cagccgggct atgccgagcc gtacctcctg cccgaattcc
     5101 acggcgaaga gggcgaaggc ttagccggta gcaacggccc cttgcgctat tccgatctcg
     5161 acgaaatgtt tgaagatgcc gcctcatcat ccaaacgcag cacggctcta cctccatgat
     5221 tcagcgcagg ctcaaattgg gttataaccg cgcaggtcgc ctcatggacc aactcgaatc
     5281 ggccggcatc gtaggcgccg ccgacggcag caaggcccgc gaggtgctca tttacgacga
     5341 gatggagttg aaccggtttt tggaggattt gaagaggcgg taataaataa aattttcatg
     5401 acacgaatca ttttcacact gacattcagc ctgttctgtg gcttactgtt ttcacaaaca
     5461 gccgacgagc tgctccgcga cttgcaacag gccaccaccg ccaaagaaaa aatgctcatt
     5521 aattttcaga ttgctgagac ctacatgcgc ggcggagacc gggaaaaagc cattgatttt
     5581 gccagaaccg cccatcagtc ttccatagag caaagcaaca acggcatggc agcccgctcg
     5641 gcctacctca tcggcgatgc ttacgagcgc ctgcgcaacg accgaaacgc cgaaacctgg
     5701 tacaaaacct gtctcaattt tgccaaaact gccggcgact tcgacctcat cgtacagagt
     5761 gtgaaaaaac gcagcaaaat tgccaccaaa gacggcaact accgccgcgc ctacgaaatc
     5821 aatcaggaag ccttcgattt tttcagcggt aaaggcacca gcatttccga tctggaaggc
     5881 aagtacgaaa atcaaaaggt cattctggat cgcgaaaaac gggtgctcgt agaagagaaa
     5941 aaccggctgg agcgcgaaat tgcctctatg gccgcagaac gcgaccaact cagtaccgac
     6001 aaaacacatt tagagtcgcg gcagcaggaa ttggtacagc aaaaggccaa agtggaagag
     6061 cagatttctg aaaaggaaca ggcgctggtt tcagtagccg aagaaaagcg caaagtggaa
     6121 gaagtggcac agcaaaaaga agctgccctg aaaaatctct cccgcgagaa actcatacag
     6181 gaagctgccc tcaacgctgc caaaaaagaa agcgcagaag ccgaactagc tgtgcaacag
     6241 gagaaaagcc tccgcgagca ggagaaaagc ctccgcgaaa aaacgctcct ccaggcattg
     6301 ctgggtggcg caataggcgc tttgatcctg ttttttatga ccctgctgta cctgtcgagc
     6361 cgcagggcca agtccagtct acagcgcaaa aataaactca ttgagatgga acgcgagcgc
     6421 tccgatgaac tgttgttcaa catcttgccc aagtctatcg gcgaggaatt gaaggaattc
     6481 ggcaaggccc gcgcccgcaa gttcgagcag gctacggtgc tgttcaccga tttcaaaaac
     6541 ttcactaaaa tatcggaaac cctcagtccg gaagacctcg tggccgaaat tgaccgctgt
     6601 ttcaaaggtt tcgattttat catctcgcag tatcccgaca ttgaaaaaat aaagaccatc
     6661 ggcgacgctt atatgtgtgc ctcaggtttg aacgaagggg gcagttcgcc cgacaacctg
     6721 ctgcgcgcag ccttagaaat gcaagaattt ttggatgagc aaaaggtaga gcgccaacgc
     6781 ttgggcaaac cctacttcga ggcccgcatc ggcctgcata caggcccggt ggtggccggc
     6841 gtggtgggcg tcaataaatt tgcctacgac atctggggcg acacggtgaa cattgccgcc
     6901 cgcatggagg cccaatgcga agagggccgc atcaatattt cagaaactac ctacaacctc
     6961 gtgcgctact catttgaatg cacttaccgg ggtaaagtag aggccaaaaa taaaggcgcc
     7021 atagatatgt actatgtaga gcgggaattg gccggcagcg gtgtgtattc gtgaggcggg
     7081 cgagtaaaaa actgctcagg tagtggactc actttaatga acagtctctc gttttttttc
     7141 gctcctgaaa gtttttttga aaaaatttaa atgcccggaa actttcaggg catagcctcc
     7201 tgttattgcc ccgttcttgc atctgaaatg ccgcaggaac gaaacatcaa aaacactttt
     7261 tcggaaaaac aacaaaatct tccaaccatg ttgcgcaacc acatttccat caccacgcac
     7321 ttcgtccggc cgtgcgaccc ctcagattgc tgcgggttcc gaccgtttcg gaagtgttgc
     7381 ccccgcgacg ggcagcaggt ggatgggttg cgtaaaaagt tacgccccct cggagccggc
     7441 tgaaaacggc gcctcctcaa aaacttcttc tcttttttct tgcactatgc agcgcgaaca
     7501 gcactggtac tgatcgaagg cgttacttcg catccgaatc agaaaaacac gcctcgaatt
     7561 tttgtccttg ctgttcctgt tgttcagtga tttgaaaaac agccctttag cccaaccggc
     7621 agaggcaatg gtttcagaag ccattcagtt tcggttcgaa tccgaaaggg gctacctgta
     7681 tatggtgaca tggcgcaacg gcaagcgcac ccgactgtct atcggaaggt tgtgggttca
     7741 aatcccattg tcaccgcttg taatagcccg gtcgttcaat ggcaggacgg gggaatacga
     7801 atccccaaac gaaggttcga ctccttccct ggcttcgtag cagtgagagt ggtttaatgg
     7861 cagaacgccg gtatgccaag ccggagacag gagttcgatt ctcctctttc gctctgtatt
     7921 ttgcgcggtg gtgtaacaag caacacacgg ctctcataag gccgtagatg cgggtgtgaa
     7981 tcccgcctgc gcaaccactt gtaaaggtaa ctcaaccggc agagtaccgg tcttttaaac
     8041 cgggagttgt gggttcaaat ccctcccttt acacagttta caggaggtgt agctcaatgg
     8101 tagagcgccc cggtgaaatc ggggtagtgg tggttcaagt ccatccgtct ccacgaggca
     8161 atttttccag gctctgtgcc cgaccggtaa tgggggcagt ttgcaaaact gttgtatgtg
     8221 ggttcaaatc ccaccggggc ctctttgagt gatagtgagt tttcgggtta cttcggtcaa
     8281 cacaagggtt cgagtcccaa atcccttgaa caaaggggtt aaacaatgcc cgggacggtt
     8341 ttcttcactc aaaaaaatgt tttcgatagc gggtagaacc cgcaggatag tccgcacgag
     8401 gtagaacctc gcgcgggcat acacacaggg aagaggtgca ataggaagca tgagcgcttt
     8461 ggaagcgcgg ggctgtgggt tcgaatcccg ccttccctac cagtaagatc aggcgatact
     8521 gacgacaagt gatacttcgt agctcatcca ggttagagca tttgtttagg gtacaaatgg
     8581 tagcgggttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn naaaaccatt ttaaaaactt gcaatcatgg cacgtttcaa cttttctgtc
     8701 ggcaaaaaaa acaagacggt caaccgcgcc ggcggaaatg cctttgtgca atcgcccgaa
     8761 atgcaactgg cttctttgct gctcacttcc tttgcgcaag accagttcta ccgcgacacg
     8821 aagcaaacgt tcagcgatct gagcgctttg ctggccaagg tgaaccctga gttcgccgct
     8881 aaggccgccg tgtatgcccg caccgaattc ggtatgcgca gcatcacgca catactggct
     8941 gccgagctgg gcatgtacgc atcgggcaag gcctggggca aggcgttcta cgaaaaaata
     9001 gtactgcgcc ccgacgacat gaccgaaatc gcgtcgctga tgctggcgga aaatggcgct
     9061 aaactgccca acgccctgcg caagggtttt gccaaagctt tcgacaagtt cgacggctac
     9121 caattggcca aataccgcgc cgaaggcaag gccgtgaaat tggtggactt ggtcaacctc
     9181 gtgcgcccgg tgccggtggc ccgcaatgcc gaagcgctgc gtcaattgac ggcaggcgaa
     9241 ctgcgcaaca ccgatacctg ggaagccaag ctgacgaaag ctggcgcaac tgctgaaaca
     9301 gatgccgaaa aggcagaact gaaagccgag gcttgggcag aattgttgcg cacgcgcaaa
     9361 atcggctact tcgcgctcct gcgcaacctc cgcaacatcg ccgaacaagc gccggaactc
     9421 gttccgacgg cttgcgaatt gctgaccgac gaacgactca tccgcaaatc gctggtgttg
     9481 ccgttccgtt tcagcacggc attggaagcg ctggaaactg ccaacatcgc cgacaagcgt
     9541 tctttggtca aggccctgaa ccgcgccgtg gacctctcat tggccaacgt gccggtattg
     9601 ccgggccgca cactggtggt tttggatgac tccggctcnn nnnnnnnnnn tccgatccaa
     9661 gtcggatcta tgtttgcagc cgtgctgtac cgcagcaacg acgccgacct gatgcgtttc
     9721 tcagacagcg cctcctacct gcgtcaaaac ccggacaatc cggtaatgac catcgcagag
     9781 gcattggtaa aaaatgccta catggccggc acgaacttca acgccatctt cgagaaggcc
     9841 aagcgtgcgt acgaccgcat cattatcttg tccgacatgc aaggctgggt gggcggcggc
     9901 gcacctacga aggctttcgc cgactacaag gctcgctaca acgccgatcc tttcgtgtat
     9961 tcgttcgacc tgcaaggcta cggctcgctg cagttcccag aatcgaaagt gtttgccatc
    10021 gccggtttta gcgaaaaagt gctggagctg atgggccaat tggaacgcga tccgcaagcg
    10081 ctgatccgcg ccattgaggc agtagaactg gcctgatgta caacattcat gagcatgatt
    10141 ttgcacaagc aggcgacttt ccgttgggag gtcgcctttg ctgttttctt acaatcgtta
    10201 ctattcagtc gccctgcaaa aagctgcgac cacgccacag gcgtggtacg ggctgcgagt
    10261 ttgattttcc aaaatgaaat agggt
//
LOCUS       DAORLF010000129        10250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3513_length_10250_cov_1.949583, whole genome shotgun sequence.
ACCESSION   DAORLF010000129 DAORLF010000000
VERSION     DAORLF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_10250_cov_1.949583"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="tyrS"
                     /locus_tag="PK198_11690"
     CDS             complement(<1..630)
                     /gene="tyrS"
                     /locus_tag="PK198_11690"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRF39438.1"
                     /translation="MNFIEELTWRGLLKDATPGVEELLNNQMVTGYIGFDPTAPSLTI
                     GNYVPIMLLTLFQRSGHQPVVLMGGATGRIGDPSGRDTERELKSAEELDANLAHQAEQ
                     FKKLLNFEDGPNKAIMVNNFDFYQGMSVLDFLRDAGKTLTVNYMLSKDAVKNRMEGGI
                     SFTEFSYQLLQAYDFEVLFKKYNCVLQMGGSDQWGNIVSGIDLGRRMGTH"
     gene            complement(725..1372)
                     /locus_tag="PK198_11695"
     CDS             complement(725..1372)
                     /locus_tag="PK198_11695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF39439.1"
                     /translation="MSTDTPGHSKVKHVAIAGNIGAGKTTLCTQLGKQFGWEVHYEST
                     DDNPYLSDFYNDMHRWSFNLQIYFLNSRYRQILKILQGDRTVIQDRTIYEDAYIFAPN
                     LHDMGLMSSRDFNNYFSLFRTMSSQVQAPDLLIYLRAGIPTLVSHIQSRGRDYEGNMS
                     LDYLKHLNERYENWISGYQEGRLLIINADDIDFETNVEDLGKVVDMVNAELHGLF"
     assembly_gap    1410..1527
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1528..1785
                     /gene="gatC"
                     /locus_tag="PK198_11700"
     CDS             <1528..1785
                     /gene="gatC"
                     /locus_tag="PK198_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRF39440.1"
                     /translation="EQLAKLELDDEERKHLIQELNHILELVEQMNRLDTSAVEPLTYV
                     NTHVNRFREDEISGQVERSAALRSAPDSDGSFFKVPKVIDL"
     gene            1798..2511
                     /locus_tag="PK198_11705"
     CDS             1798..2511
                     /locus_tag="PK198_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF39441.1"
                     /translation="MSSVISVKDLKKTYIMGLTQVNALQSVSIDISKNEYVALMGPSG
                     SGKSTLMNLLGCLDTPTSGEYWLNGTNVSSMSDGELAEVRNKEIGFVFQTFNLLPRLS
                     ALENVALPLVYAGMSKSHRLERAEEVLKSVGLADRMDHKPNELSGGQRQRVAIARALV
                     NKPSIILADEPTGNLDTKTSIEIMGILEEIHNQGNTIILVTHEADIAEHAHRIVRLRD
                     GLVETDVLNQKIVSALAGA"
     gene            complement(2600..3082)
                     /locus_tag="PK198_11710"
     CDS             complement(2600..3082)
                     /locus_tag="PK198_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF39442.1"
                     /translation="MRKFKATLEIIGINPFVFVPEKILAHIFEQAGKDKGYIPICGTI
                     NGKAYIQTLLRFRGEWRLYINMAMLKDSPKRIGEEIEVTIQYDPVERTLTPHPELVKA
                     INENELAKSVFDTLAPSKQKEMIRYIAVLKTEKSITIGIEKIIGFLTGKNKFAGREKP
                     "
     gene            complement(3336..>4721)
                     /locus_tag="PK198_11715"
     CDS             complement(3336..>4721)
                     /locus_tag="PK198_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39443.1"
                     /translation="DKNADGFTDVAQQKRISIFNKWQWQRPGNRVAQLGARYVREDRW
                     GGQTNWTPAFRGSDEIYGESIYTNRLELFGQYQLPIEAPVFVQVSYNVHDQDSYYGDV
                     RYDARQEIGFVQSYWDKKLGKHRLLAGAALRHTRYDDNSPATEQAERTLLPGVFVQHE
                     WEVGEHDHLLGGLRLDWHPKHDAVWSPRLALHRHLSENQGIRAGLGSGFRVVSLFTED
                     HAALSGAREVVIAEALNPERSWSGNLNYELKIPAERYFLRFDVSAFYTLFLNRILPDY
                     DTDPQKIIYANLDGRATTRGFTLQAGYSDGLPLRIDAGISYMEVFQRDAEGEKTVQIR
                     APKWSGTFSANYTHAPSRLSFDLTGNWFGPQRLPIVPNDFRPEYSPWFALVNVQVSRK
                     FKNGWELYGGLKNLLNFVPQHPILRPFDPFDRTADDPLSNPFGYTFDPSYNFAPLQSI
                     RGFMGMRWVLR"
     assembly_gap    4722..4849
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4850..>5132)
                     /locus_tag="PK198_11720"
     CDS             complement(<4850..>5132)
                     /locus_tag="PK198_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF39444.1"
                     /translation="LSGVQPVINCNVCNTGDIQINGIEGVYTQILLDGMPIVSGLGTV
                     YGLMGIPNSLIERIEVVKGPAGTLYGSEAMAGQINIITKNSLSAPRFSLD"
     assembly_gap    5133..5204
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5205..>5551)
                     /locus_tag="PK198_11725"
     CDS             complement(<5205..>5551)
                     /locus_tag="PK198_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39445.1"
                     /translation="LFLLILATSPAFAQMAGARLLCRVETDDHALEFATVELSELRIG
                     STTDSLGRAFFEQVPPGTYEVVASFVGYFSQKKTIQLSPGQSLELRFHLTAQTLQEVV
                     VTGALREMSRSDS"
     assembly_gap    5552..5571
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5686..7149)
                     /locus_tag="PK198_11730"
     CDS             complement(5686..7149)
                     /locus_tag="PK198_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39446.1"
                     /translation="MKIQISIIALMLCSLWSTAQDSTIWSFRSTHLSYRIADMQYFKD
                     HAYVLRVNYDGFVYAKKNKSVIVQFDQRLHYIKEFKIALNYEGKRAEPRKMIAIKDKL
                     YLLFTGYKDNEELLLLYEIDPQTLTFPNPPIVLGTVRSSRSQIWMAQSKNGRFFSVLT
                     YWGEKDKSTAYLTCYDHNFQRQYEEKKDLELKYYYAGCKKLLVDNEGNTFSIMESPPN
                     YTNPPVLWQRYTEGESIVHTLAGKQKGLKIRNFTATALHEIVLLAGYYTEQDPNDDRV
                     KGITFLAYDKQNKGDHKQVQVELPRDLSKYTRLSSITRYREDENEIPALTAIDIAEWE
                     GHGYLVIGQKQYYKMVSTGRTGDRYPTYTDIITFVTLLDYSLSRVKWAVPVCKYHESV
                     YEDPGVFVGLKEDKVHVFFNSNDSNNAKHKDDPPLYGTGFIQMTINPEGKYSTKVLHN
                     DPDGLKIYPRLIKVIDEQSLFVVAGKTYNEKFGIIKW"
     assembly_gap    7213..7267
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7268..>7955)
                     /locus_tag="PK198_11735"
     CDS             complement(<7268..>7955)
                     /locus_tag="PK198_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF39447.1"
                     /translation="TTGIADPASIAQVFLGDRVVERYFDLRNVICLADAGYIEDWLEN
                     TDEARRQIAAADMVLLNKADKVNPTYLATLEQELRGINPYLHVYTGEQGVFPVNEIMD
                     IQQIAENGAVERTRQIDGDHRHLLHDISTFTLRFSEPFQLNTLGHEMLRLVNLYQHQI
                     YRIKGVIATEGNPCRLILQSVRNTLALSDGAPWDEGESRESKIVFIGKGVERHIIEGI
                     FKRHLVAPKAD"
     assembly_gap    7956..8078
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8299..8715)
                     /locus_tag="PK198_11740"
     CDS             complement(8299..8715)
                     /locus_tag="PK198_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39448.1"
                     /translation="MVKKYGEETDALRSDPALKLTWRTLLSIKYNLKYFEEIKMEMYA
                     PVFSDTLKKLDGKFVEVEGYVIPLSATEYVLSASPYASCFFCGKASPASVMNLRFEKP
                     GKKYNMDAFVKFQGVLQLNYDDPNEFYYALVNAKEP"
     gene            complement(8878..10107)
                     /locus_tag="PK198_11745"
     CDS             complement(8878..10107)
                     /locus_tag="PK198_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF39449.1"
                     /translation="MDETILTSHNHESFSKKTTGYGTQRLGAGKTTLLNHILHNKEGI
                     KAAVIVNDMSEVNIDSQLVDASGALVRSEEKLVEMSNGCICCTLREDLILEVERLAKE
                     ERFDYLFIESTGISEPAPVAQTFHFVSEESGVDLSRFAYIDAMVTVVDCCNFLKDYGS
                     NERLPDRNLNEAPADDRPIVNLLTDQIEFANILLLNKTDLVSEDDIQFLEGLMKKLNP
                     GARIIRSVMGQVPVSELVHTDLFDLEAAENAAGWLKELEGEHIPETETYGFNSFVFRA
                     RKPFHPERFYKFLNSDFPGNIFRSKGLFWLASRPAVALSWSQAGGSLRYDEIGTWWAS
                     KSVLERIHYPAFFEHQESIEKNWDTVWGDRKQELVFIGQQMDEEGITRALERCLLRDW
                     ELEMYHEGEAFQDPFPT"
BASE COUNT         2278 a         2369 c         2513 g         2574 t
ORIGIN      
        1 atgcgtgccc atgcggcggc cgaggtcgat gccggagacg atgttgcccc actgatcgga
       61 gccgcccatt tgcaacacgc agttgtattt tttgaacaat acttcaaaat cgtaagcctg
      121 caacaactgg taactgaact ctgtaaaaga aatgccgccc tccatccggt ttttaaccgc
      181 gtctttggag agcatatagt tgactgtcag tgttttacct gcgtctctca ggaagtcgag
      241 tacgctcata ccctgataaa aatcgaagtt gtttaccatg atggctttgt tggggccgtc
      301 ttcaaaattg agcagttttt tgaattgctc tgcctgatgt gcgaggttgg catccaattc
      361 ttcggccgat ttgagttcgc gctcggtatc ccgtccggag gggtcgccga tgcgcccggt
      421 agcgccgccc atgagcacta ccggttggtg gccgctgcgc tgaaacaggg tgagcagcat
      481 gatgggcaca taattgccga tggtgagcga cggcgccgta gggtcgaagc cgatgtagcc
      541 ggtcaccatt tggttgttga ggagttcttc tacgccgggg gttgcgtctt tgagcaggcc
      601 cctccaggtc aattcttcaa tgaagttcat gagtgttgta atgtgatgat gagtttttta
      661 tttgatagta ggcggtgggc ggtgcaaaat tggaaatccg gaatacgaaa tccggaatcc
      721 gaaatcaaaa caacccatgc aactccgcat tgaccatatc caccactttg ccgaggtctt
      781 ccacattggt ttcaaaatca atatcatcgg cattgatgat gagcaggcgg ccctcctgat
      841 agcccgaaat ccagttttcg tagcgctcgt tgaggtgctt gagataatcg aggctcatgt
      901 tgccttcgta gtcgcggccc cttgactgaa tgtgcgaaac caaggtagga atgcccgcgc
      961 gaaggtagat gagcaaatcg ggcgcctgta cttgtgaaga catggtgcgg aacaggctga
     1021 aataattgtt gaaatcgcgc gaggacatca gccccatatc gtggaggttg ggcgcaaaaa
     1081 tataagcatc ctcatagatg gtgcgatcct gaatgacggt gcggtcgccc tgaaggattt
     1141 tgagaatttg ccggtatcgg ctgttgagga agtaaatctg aagattgaac gaccagcggt
     1201 gcatgtcgtt gtaaaaatcg ctcagataag ggttgtcgtc ggtgctttcg taatgcactt
     1261 cccatccgaa ttgcttgccc aattgggtgc aaagcgtggt tttaccggcg ccgatgttgc
     1321 cggcaatggc gacgtgtttt actttagaat gtccgggtgt gtctgttgac ataatgatcg
     1381 ggttgaaaaa aaacgtatct tggcggcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa cagctcgcca agctggaatt ggacgacgaa
     1561 gaacgcaaac acctgattca ggaactcaat catatcctgg agttggtaga gcagatgaat
     1621 cgtttagaca cctccgcagt agagccgctg acttacgtca acacgcacgt caaccgtttt
     1681 agggaagacg aaatcagcgg acaggtggaa cgaagcgctg ctttgcgctc ggcgcccgat
     1741 tccgacggca gcttttttaa agtacccaaa gtcattgatc tttaatcttt tgcaactatg
     1801 tcgtctgtta tttccgtcaa agacctgaag aaaacctaca tcatgggcct cacacaggtc
     1861 aatgcacttc agtcggtcag cattgatatt tccaaaaacg aatatgtggc gctcatgggg
     1921 ccttcgggtt cgggcaagtc cacactgatg aacctgctgg gctgcctcga tacgcctacc
     1981 tccggcgaat actggctcaa tggcaccaac gtgagcagca tgagcgacgg cgagctggcg
     2041 gaagtgcgca acaaagaaat cggattcgtt ttccaaacct tcaacctgct gccccgcctt
     2101 tccgctttgg aaaatgtggc gctgcctttg gtgtatgccg gcatgtcgaa atctcatcgt
     2161 ttggaaagag ccgaggaagt gctcaaatcg gtaggattgg ccgaccgcat ggatcacaag
     2221 cccaacgaac tgtcgggcgg tcaaagacaa cgcgtggcca tcgcccgcgc attggtcaac
     2281 aaaccttcca tcattctggc cgacgaaccc accggcaact tggataccaa aacctccatc
     2341 gagatcatgg ggatattgga agagattcat aaccaaggga ataccattat tctcgttacc
     2401 cacgaagccg atattgccga acacgcgcac cgtatcgttc gcctccgcga cggcttggtg
     2461 gagacagatg ttttgaatca aaaaatcgtg tcggcgctgg ccggagcata aacgaaggtg
     2521 gtccttcatt ctgctatttc gctcatatag cagtctaata aatatcattt tgcagctttc
     2581 tgaatactgc gactgctgct tatggcttct ctcgcccggc aaacttgttt ttgccggtca
     2641 aaaagccaat gattttttcg ataccgatag tgatgctttt ttctgttttt aaaactgcaa
     2701 tatatcggat catttctttc tgtttagaag gcgccagcgt gtcaaatacg ctttttgcca
     2761 attcattttc attgattgct tttacaagtt ctggatgtgg cgtcaaggtt ctttcaacag
     2821 ggtcgtactg tattgtaact tctatttctt cgccgatacg tttaggtgaa tctttcagca
     2881 tggccatgtt gatgtacagc ctccattcgc ctctgaaccg taataaagtt tggatatatg
     2941 cttttccatt gatcgtcccg caaattggga tatagccttt gtccttacct gcttgctcaa
     3001 aaatatgtgc aagaattttt tccggcacaa acacaaaagg attgatgcct attatttcca
     3061 atgttgcttt gaactttcgc atttgttgtg ctggtttctt ttttagtaac tatacagccg
     3121 gtcccccaaa gctacgacca cgccggggcg tggcacgagc tatgagtttg tttactcgtc
     3181 gggaaattag attttccatt tggggaaatt tcaaaatctc cctctgaaaa cgagcgtttt
     3241 ttttcattct ggaaaatcaa actcgcggct cgtagctcgc ggctcgcagc tttttgcagg
     3301 gcgctgaata gtaaccaaaa taataattct cagctttatc tcagcaccca tcgcatcccc
     3361 ataaaacctc tgatgctctg caaaggcgca aagttgtacg aagggtcaaa cgtatagccg
     3421 aaaggattgc tcagcgggtc gtctgccgtt ctgtcgaatg gatcgaaagg gcgcaggatc
     3481 gggtgctgcg gcacgaaatt gagcaggttt ttgaggccgc cgtacaattc ccagccattt
     3541 ttaaatttcc gcgacacctg cacattcacc aaagcgaacc agggcgagta ctccggccgg
     3601 aagtcgttgg gtacaatggg caggcgctgc ggcccaaacc agttgccggt cagatcaaaa
     3661 ctcagccggc tgggtgcgtg cgtgtaattg gccgaaaaag tgcccgacca tttcggagcg
     3721 cgtatttgca cggttttttc accttccgca tcccgttgaa atacctccat atagctgatg
     3781 ccggcgtcta tgcgcagcgg cagcccatcg gaatagccgg cctgtaaggt gaagcctcgg
     3841 gtagtggccc gtccgtccaa attggcataa atgatctttt gcgggtcggt gtcgtagtcg
     3901 ggcaggatgc ggttgagaaa cagggtgtaa aacgccgaca catcgaagcg gaggaaatag
     3961 cgctcggcgg gtattttgag ttcgtaattc aggttgccgc tccaggaacg ctccgggttc
     4021 aacgcctcgg caatgactac ctcccgcgcc ccgctgaggg ccgcatggtc ttcggtgaac
     4081 aggctcacca cccgaaaccc gctgccaagt cccgctctga tgccctgatt ttcagacaaa
     4141 tgccggtgca atgccaatcg gggcgaccac accgcgtcgt gcttcgggtg ccagtccaaa
     4201 cgcaatccgc ccaagaggtg atcgtgctcg cctacttccc actcgtgttg cacaaacacg
     4261 cctggcaaca gggtgcgctc ggcctgttcg gtagcgggcg agttgtcgtc gtagcgggta
     4321 tgccgcagcg ccgcgccggc caacaggcgg tgtttgccta actttttgtc ccagtaagat
     4381 tgtacaaagc cgatttcctg ccgggcatcg tatcgcacat cgccgtagta ggaatcctga
     4441 tcgtgtacat tgtatgaaac ctgcacaaaa acaggcgctt ctatcggaag ctgatattgg
     4501 ccgaacaact ccaaccggtt ggtatagatg ctctcgccat agatttcatc gctgccgcga
     4561 aaagccggcg tccagttggt ttggccgccc cagcggtctt cgcgcacata gcgcgcaccc
     4621 aattgcgcca cccggttgcc gggccgctgc cactgccatt tgttgaagat ggaaatgcgt
     4681 ttttgctgcg ctacatcggt gaacccatct gcgtttttgt cnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcgagggaaa
     4861 aacgcggcgc ggagaggctg tttttggtga tgatgttgat ttggccggcc atggcttccg
     4921 aaccgtagag cgtgccggcg gggcctttca ccacctcgat gcgctcgatg aggctgttgg
     4981 gaatgcccat gaggccatac accgtgccga ggccgctgac gatgggcatg ccgtcgagga
     5041 ggatttgtgt gtaaacgccc tcaatgccgt tgatctggat gtcgccggtg ttgcacacat
     5101 tgcagttgat aaccggctgc acgccgctga gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggctgt cggagcggct
     5221 catttcgcgc agggcgccgg tgacgacgac ttcctgcagg gtttgggcgg tgagatgaaa
     5281 acggagttcc agactttggc ccggcgatag ttggatggtc tttttttgag aaaaatatcc
     5341 tacaaaagaa gccaccacct cgtaagtgcc gggcggaacc tgctcgaaaa atgccctgcc
     5401 gaggctatcg gtggtgctgc cgatgcggag ttcgctcagt tccacggtgg caaattcgag
     5461 ggcatggtcg tcggtttcca cgcggcagag gagtctcgcg ccggccattt gcgcgaaagc
     5521 ggggctggtg gcgaggatga ggaggaagag gnnnnnnnnn nnnnnnnnnn natgggcggg
     5581 tagaacccgc cggatagagg caccacgcct tgcgtggtaa acatgagatg gaatctcgcg
     5641 ccagcgtgag tagaaggtgt tttttcattt tgggtgctat caatttcacc atttaataat
     5701 gccgaacttt tcattgtaag ttttgccggc cactacaaaa agcgactgct catcaatcac
     5761 ttttattaat cgtggataaa tcttcagccc gtctggatca ttgtgcaaaa ctttggtgct
     5821 gtatttacct tcagggttta tagtcatctg gatgaaacca gttccgtaga gaggaggatc
     5881 atctttgtgt ttcgcattgt ttgaatcatt ggaattgaaa aaaacatgca ctttatcctc
     5941 ctttaatcct acaaaaacgc cggggtcctc atacacagat tcatggtatt tacatacagg
     6001 aactgcccat ttcactctgg acaaggagta atccaacaaa gtaacaaaag taattatatc
     6061 ggtataagta ggatagcggt ctccggttcg gcctgtgctt accattttat aatattgctt
     6121 ttggccgatt accaaatagc catgtccttc ccactctgct atgtctatgg cggttagcgc
     6181 ggggatttca ttttcatctt ctctgtatct ggtgatggat gaaagccgtg tgtattttga
     6241 caggtctctg ggaagctcta cctgcacttg cttgtgatcg cccttgtttt gcttgtcgta
     6301 tgccagaaaa gtgatgcctt ttaccctgtc gtcgtttggg tcttgctctg tgtaataacc
     6361 tgccagaagt acaatttcgt gtaatgctgt tgctgtgaag tttctgattt ttagcccttt
     6421 ctgttttcca gccagcgtgt gtacaatact ttcaccctca gtgtatcttt gccaaagcac
     6481 aggcgggttc gtatagttgg gcggactttc catgatggaa aatgtattgc cctcattgtc
     6541 caccaacaac tttttgcaac cagcgtagta gtactttagc tccaggtctt ttttttcctc
     6601 atattggcgt tggaaattat gatcataaca agtgagataa gcagtgcttt tgtctttttc
     6661 gccccaataa gtaagcacgg agaagaaccg gccgtttttc gattgtgcca tccatatttg
     6721 gctgcgggag cttcttacag tgccgagcac aataggggga ttgggaaaag tgagggtctg
     6781 cggatcaatt tcatacagca gaagtagttc ttcattgtct ttatagccgg taaaaagtaa
     6841 gtataacttg tccttaattg caatcatttt gcggggttcc gcccgtttgc cctcataatt
     6901 cagcgcgatt ttgaactctt ttatgtaatg gagacgctgg tcaaattgaa cgatgacgct
     6961 tttatttttt ttagcataaa caaacccgtc ataattgact ctcagcacat aagcgtggtc
     7021 tttaaaatat tgcatgtctg caattcggta tgaaaggtgt gtggaccgaa aagaccatat
     7081 cgtcgagtct tgcgctgtgc tccataaaga gcagagcatg agggcaatga tggagatttg
     7141 aattttcatg ttgagcatgt ttggggcaaa tgtaggtaaa aaaactgcct catgccggtt
     7201 ttattccgct gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnatc tgccttgggc gctacgaggt gccgtttgaa aatgccctcg atgatgtggc
     7321 gctctacgcc tttgccgata aacacgattt tgctttcgcg actttcgcct tcgtcccagg
     7381 gcgcgccgtc ggagagggct aaggtgttgc gcaccgattg caggatgagg cgacagggat
     7441 tgccctcggt agcaatgacg cccttgatgc ggtaaatctg gtgttggtac aaattgacta
     7501 agcgcagcat ttcgtggccc agggtgttga gttgaaaagg ctcggaaaaa cgcagcgtga
     7561 aggtgctgat gtcgtgcagc aggtgccggt gatcgccgtc tatctgccgg gtgcgttcta
     7621 cggcgccgtt ttctgctatt tgctgaatat ccatgatttc gttgaccgga aaaacgcctt
     7681 gctctccggt atagacgtgc agataggggt tgatgccgcg cagttcctgt tccagcgtgg
     7741 cgaggtaagt gggattcact ttgtcggcct tattgagcag caccatatcg gcggctgcga
     7801 tttgccggcg ggcctcgtcg gtgttttcga gccagtcttc gatgtagccc gcatcggcca
     7861 gacaaatcac attgcggagg tcgaaatagc gttctaccac gcggtcgcca aggaacacct
     7921 gcgcgatgga ggcgggatcg gcaatgccgg tggttnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gtctatgttg acgtcgccca
     8101 cttcgttttc gatgacaaaa atgcgctccg gcagccgctc ccggatgagg tggttgagga
     8161 aggtggtttt gcccgaaccg agaaaaccgc tgatgatgtg tacgggtatt ctgttgctca
     8221 tggatttatt attgttttgc acagaaacag tgcgaacgcg cattttgctc aagcaggcat
     8281 ccacaataca cctgattctt atggctcctt ggcatttacc aacgcgtagt aaaattcatt
     8341 ggggtcgtcg tagttcagtt gtaaaactcc ttggaatttg acaaatgcgt ccatgttgta
     8401 cttttttccg ggcttctcaa acctgagatt catcaccgag gccgggcttg ctttgccaca
     8461 aaaaaagcag gacgcatacg ggctggccga aagcacatat tcagtagcgg agagcggaat
     8521 cacataacct tctacttcta cgaatttgcc gtccaatttt tttaatgtat cgctgaaaac
     8581 cggcgcgtac atctccattt tgatctcctc gaagtacttg agattgtatt tgatagagag
     8641 caaagtgcgc caggtaagtt tcaaagcagg atcggagcgt aaggcatctg tttcttcccc
     8701 gtactttttt accattgcag gaacttgctc cggtctgcta ttgtcggcta aacttccggc
     8761 gctcaatagc gccaacatca aacatttcaa agttatcaac atgcatgtta ttttaatgcc
     8821 aaagatacct catctcaatt gagcgaattc aaaaaaaaaa tgcaaatccg ttgatcatca
     8881 agtcggaaac gggtcttgaa acgcctctcc ctcgtggtac atttctaatt cccaatcgcg
     8941 cagcaggcag cgttccagag cgcgggtgat gccctcctca tccatttgtt gcccaatgaa
     9001 taccaactcc tgcttgcggt cgccccaaac agtatcccaa ttcttttcaa tgctctcctg
     9061 atgctcaaaa aatgcagggt aatgaatgcg ctccagcacc gatttggatg cccaccaggt
     9121 accgatttcg tcatatcgaa gcgagccgcc ggcctgactc cacgacaaag ctacagcagg
     9181 ccgggatgcc aaccagaaca agcctttgga gcggaagata ttgccgggaa aatcgctgtt
     9241 caggaacttg taaaatcgct ccgggtggaa gggttttctg gcccggaaca caaatgaatt
     9301 gaagccataa gtttctgtct ccggaatgtg ctcgccttcg agttctttga gccagccggc
     9361 tgcattttct gctgcttcga gatcgaaaag atcggtgtgt accaactcgg aaacaggcac
     9421 ttggcccatc acagaccgga tgatgcgcgc tccgggattg agttttttca tcagcccctc
     9481 caaaaattgt atatcatctt cggaaaccag atcggtttta ttgagcaaaa ggatgttggc
     9541 aaactcgatc tgatcggtca gcaaattcac aatcgggcga tcatcggcgg gggcttcatt
     9601 caggttgcgg tcgggcaggc gctcattgct gccgtagtct ttcaggaaat tgcagcagtc
     9661 taccactgtt accatcgcat caatgtaggc aaaccgcgat aggtccacgc cgctttcttc
     9721 actgacaaag tgaaaagtct gggctacggg cgccggttcg ctgatgccgg tgctttcgat
     9781 aaaaaggtag tcgaagcgct cttctttagc taagcgctct acttccaaaa tcaggtcttc
     9841 gcgcagggtg caacaaatac aaccattcga catctccacg agtttttctt ccgaacgtac
     9901 caatgctccc gaagcgtcca ccaattggga gtcaatgttt acttccgaca tatcgttgac
     9961 aatgacggcc gctttgatgc cttctttgtt gtgcaaaatg tgattaagta aagttgtttt
    10021 tccggcacca agccgctgag taccgtaacc ggtagttttt ttggaaaatg attcatggtt
    10081 gtgtgaagtt aaaattgttt catccaattg atcttggcaa tgacgccggt attctgaaac
    10141 cggttcagcc ccagatcgct gcgcgtatca ttgaatacca aaaacaaaaa agacaaaggc
    10201 tggtactccc aactgaggcg ggcattccag cgaccggttt tgtccaaaga
//
LOCUS       DAORLF010000130        10209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3530_length_10209_cov_2.146937, whole genome shotgun sequence.
ACCESSION   DAORLF010000130 DAORLF010000000
VERSION     DAORLF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3530_length_10209_cov_2.146937"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>866)
                     /gene="atpG"
                     /locus_tag="PK198_11750"
     CDS             complement(47..>866)
                     /gene="atpG"
                     /locus_tag="PK198_11750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRF39450.1"
                     /translation="VVSAAKFRRAQQAIVQMRPYAEKLNKMLRNILSNMEGEADTVFG
                     QERPVHKACVVVVTSNRGLCGAFNSNVIKGAVRVINEELADVRRSGNLSILCIGKRGY
                     DFFRKRYTDCNMITDHVELFSNLNFDHVAPVARQLMDAFQAKEYDRVYVAFGHFRNAA
                     TQFPVIEQWLPVAKIKPEPGADKKRADYIFEPDMKGLLDYLVPSILQTTFHRYLLDTH
                     ASEHGARMTAMDKATENAEEMLKELLINYNKARQEAITKEILEIVGGAAALEGA"
     assembly_gap    867..876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<877..984)
                     /locus_tag="PK198_11755"
     CDS             complement(<877..984)
                     /locus_tag="PK198_11755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRF39451.1"
                     /translation="MSGSLKQVRERIRSVQNTQQITKAMKMVAAAKLKRA"
     gene            complement(1066..>1792)
                     /locus_tag="PK198_11760"
     CDS             complement(1066..>1792)
                     /locus_tag="PK198_11760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538444.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRF39452.1"
                     /translation="PGREAYPGDVFYLHSRLLERAAKVINNDDIARNMNDLPESLKKA
                     GIVKGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNAGVRPAINVGIS
                     VSRVGGNAQIKSMKKVAGTLKLDMAQYRELEAFSKFGSDLDPATVAILEKGKRLVEVL
                     KQPQYSPAPVEKQVAIIYLGTKGLLRSVPVDKIKEFEHVFLTEMERRHATILDNFRKG
                     KFDDADLKQVEALANELTMQYKK"
     assembly_gap    1793..1885
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1886..2658)
                     /locus_tag="PK198_11765"
     CDS             complement(<1886..2658)
                     /locus_tag="PK198_11765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692683.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRF39453.1"
                     /translation="MVDVKPDEISAILKQQLSGFSTSSELEEYGTVLQVGDGIARVYG
                     LNNAQAGELVVFEETGVEAIVLNLEEDNVGVVLMGSGTGIKEGSRVHRTGRIASISVG
                     DGFVGRVVNPLGLPIDGKGVITGAKYEMPLERKAPGVIYRQPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQKEFYDRGEPVYCIYVASGQKASTVANVAK
                     TLEEHGAMDYTVIVSASAADPAPLKFYAPFAGAAIGEFFR"
     gene            complement(2695..3264)
                     /gene="atpH"
                     /locus_tag="PK198_11770"
     CDS             complement(2695..3264)
                     /gene="atpH"
                     /locus_tag="PK198_11770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRF39454.1"
                     /translation="MSAYRIAVRYAKSLHDLAVEQRKLDRVLEDVQAFHTLMQNRDFQ
                     LFIKSPVIQHKRKSEVMEKMLADKFDPLTMAFLRILFNKGREKFMGEVAKEFITLFKK
                     LRGISTVKVTSAVKLEDNAIQAIEAKLKSEGITGAHTEFRTSVDADMIGGFILEVDDL
                     VYDASLQHKLETLRREFVHNPYVSQIISH"
     gene            complement(3313..3834)
                     /locus_tag="PK198_11775"
     CDS             complement(3313..3834)
                     /locus_tag="PK198_11775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538442.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRF39455.1"
                     /translation="MSQMLFLAKFSVIQPDPGQIFWTSVIFIGLWAILGPMAFKVIQK
                     ALKQRETDIQTSLDEAKRTRDEMSKLKADNEALLKLAQEERTKILREAAETKEAIINE
                     ARVKAKEEARLIVENAKELIENQKMAAIIEVKNQVGNMAIDLTERLLRANLKGNADQE
                     QLVNRLMDEMKLN"
     gene            complement(3927..4118)
                     /gene="atpE"
                     /locus_tag="PK198_11780"
     CDS             complement(3927..4118)
                     /gene="atpE"
                     /locus_tag="PK198_11780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRF39456.1"
                     /translation="MGAIGAGIAALGAGIGVGLIGSRAMDAIARQPEAVGDIRNNMIL
                     TAALVEGAALFAIVVGLLK"
     gene            complement(4241..5464)
                     /gene="atpB"
                     /locus_tag="PK198_11785"
     CDS             complement(4241..5464)
                     /gene="atpB"
                     /locus_tag="PK198_11785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRF39457.1"
                     /translation="MIYKRFILLFAAALFCVATPALAEEKHGAGHDEYDPVGAVMNHI
                     ADANEFHVWKGIHIPLPCFLYAPGQGWTVMSSGKFDHGHKAIDGYVLNHGRVNRVSDV
                     SFPTGEVHIEGIVSHKEAVTKAGKEQEEEVYYVMHEGKEYRLDSPSTLDGGILGGGIT
                     SFYDFSITKNVATMLLAAVLLVLLFSSVARGYKNNQGKAPKGIQGFIEPIFMFIRDEV
                     TRPMIGEKHYERFQPFIMTLFFFILFCNLMGLIPFFPGSANITGNLGVTLVLAVLAFL
                     VTNLNGNGHYWQHIFWMPGIPAWVKIILTPVEILGLFIKPFSLMIRLFANISAGHIII
                     LSLIGLIFIFGKNGTSIGGATVGAVIGGAFTAFMNLIELLVAFLQAFIFSILTASYIG
                     AAVDEGHHEHEEAHH"
     gene            5802..6185
                     /locus_tag="PK198_11790"
     CDS             5802..6185
                     /locus_tag="PK198_11790"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRF39458.1"
                     /translation="MRIFLWLMFLLPVSGFAQADFSGMAKALGSGNADALGQYFDQNV
                     EISIMDQEDVYAKTQAIGVVKQFFSQNAPRSFQQMHQGASKGSDAQYSIGNLTTASGT
                     FRVYLYAKSSGGKFLIQEVRFDKGS"
     gene            6212..7429
                     /locus_tag="PK198_11795"
     CDS             6212..7429
                     /locus_tag="PK198_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39459.1"
                     /translation="MALAWRIARRYLFSKRSTNAIHIITGIAVFGIAVGAAALLLVLS
                     VFNGFEDLITGMYNSFNPDLKITPAQGKTFPADSAFLSRIRAVPGVKDISCTLEEVAF
                     FEYKNSQDFGTLKGVDADYTKVSSVDSMVREGIFRLQQEDRSYAVLGLGMRNKLNVNV
                     DDVFSPLAVYMPKRKETAALEQAFRKRFIYPAGTFVIQQEFDNQYIITNLEAARDLLG
                     YEEEVSALEIKVNAGAAPQSVQGAVAALLGPEFVVKNRYQQEETFMRLMQVEKWMSFA
                     IVSLMMLMVSFNLIGALWMIVLEKQPDISIIKSMGGSDRLVRNIFLLEGLLLCVIGIA
                     TGILLAGGIYVAQKQLGLVSIPGNLVIDSYPISMRFGDLPVVAVTVLGIGLLASLPPA
                     LRAMRVPALMREE"
     gene            7487..8938
                     /locus_tag="PK198_11800"
     CDS             7487..8938
                     /locus_tag="PK198_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRF39460.1"
                     /translation="MVPVKIEHILADLFEKWAGESAYLVLPLAPSGSQRVYYRLQGPT
                     KTAIGAYNPNRKENQAFISFSRHFKARQLPVPEIYAEDLDQDVYLQEDLGSTTLYSYL
                     LQKGEYFPDYLVQIYRKAVEQLARLQIEGGKDLDYSVCYPRDAFDRQSMVWDFNTFKY
                     YFLRLAYVPFDEQALEADFQRLADFLLQADCNHFMFRDFQTRNIMIKSGEPYFIDYQG
                     GRRGALQYDLASLLYQAKAGMPEDIRQELLEHYIKTAGQFIHIDREQFIQYYHGYVLI
                     RSIQTFGTYGFRGLFERKEYFLESIPFALRNVRWILENAHLPVEMPELTRAMYHLIES
                     KRFEPFDKIKGASSLLTVTINSFSYKSGGIPEDPHGNGGGFVFDCRCINNPGRYEPYK
                     KLTGRDEPVINFLKHHSHMGDYLNSVFHLVNDAVEDYIERSFTHLSVNFGCTGGQHRS
                     VYAADALARHLKDKYGVRVELRHIEQERKNWQN"
     assembly_gap    9066..9093
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9094..>9330)
                     /locus_tag="PK198_11805"
     CDS             complement(<9094..>9330)
                     /locus_tag="PK198_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HRF39461.1"
                     /translation="YTKEPQEDFRHIMTGIRGQKKRIHFHAGTPLDAELDEIAAISGL
                     NHQIKALAQVLDRAIVANYRLWPSNYISHDLLSQT"
     assembly_gap    9331..9475
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9476..9943)
                     /locus_tag="PK198_11810"
     CDS             complement(<9476..9943)
                     /locus_tag="PK198_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF39462.1"
                     /translation="MDNIRATQDFHAQVIRPAVLRILAESSAGLTHSGFDQLLPDTPY
                     LYVSNHRDIVLDTALLNFILLEQGLTMTASAIGDNLVKLPFLLTFAKINRNFLVRRNL
                     PPRELLESSQELTAYIRHLMLEDRRSVWIAQREGRAKDGNDATNPGVLKMIGMA"
     assembly_gap    9975..10001
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2267 a         2551 c         2625 g         2463 t
ORIGIN      
        1 cctgttggtt ttttcgtata ggaacgatgt gttgatgcgc cggtttttat gcgccttcca
       61 atgcagcagc gccgcctacg atttcgagaa tttccttggt aatggcttcc tgacgagcct
      121 tgttgtaatt gataagcaac tctttgagca tctcctcggc attttctgtc gccttatcca
      181 tagcggtcat gcgggcgccg tgctccgaag cgtgggtgtc caacaggtag cgatggaagg
      241 tggtttgtaa gatgctgggc accaaataat cgagcagccc cttcatatcg ggttcaaaaa
      301 tatagtctgc gcgttttttg tctgcgcccg gttccggctt tattttcgct accggcaacc
      361 actgctctat cacagggaac tgagtagcgg cattgcggaa gtgaccaaag gccacataca
      421 cgcggtcgta ttctttggcc tggaaggcat ccatcaattg cctggcgacc ggcgccacat
      481 gatcgaaatt caggttgctg aacaactcca catgatcggt aatcatgttg caatcggtgt
      541 agcgcttgcg gaagaagtcg tagccgcgct tgccgataca aaggatgctg aggttgccgc
      601 tgcggcgcac atcggccagc tcctcgttga tgacgcgaac cgcgcctttg atgacgttgc
      661 tgttgaacgc gccgcacaag ccgcgattgg aggtaactac caccacgcaa gccttgtgta
      721 ccggacgctc ctgcccaaat accgtatcgg cctcgccttc catgttggag aggatattgc
      781 gcagcatttt gttcagcttt tcggcgtagg ggcgcatttg cacaatggcc tgttgcgccc
      841 tgcggaattt ggccgccgaa accaccnnnn nnnnnncgcg cgcttcagtt ttgcagcggc
      901 caccatcttc atggctttgg tgatctgctg cgtattctga accgagcgga tgcgctcgcg
      961 tacttgtttt aaactaccgg acatactgct cgcggttttg ttgtttccgg tggatgatgc
     1021 gaaaagctac gagtgcccgc acaccatcca ccgcagaatc aactattatt ttttatactg
     1081 catcgtcaac tcattggcaa gcgcctctac ctgcttgagg tcggcatcat cgaacttgcc
     1141 tttgcggaag ttgtcgagaa tagtggcgtg gcgacgttcc atctctgtga ggaagacatg
     1201 ctcgaactcc ttgattttgt ccaccggcac actgcgcaac aaacccttgg tgcccaaata
     1261 aataatggct acctgcttct ctacgggagc gggagagtac tgcggctgct tgaggacttc
     1321 caccaagcgc ttgccttttt cgaggatggc tacggtagcc gggtcaaggt cggaaccgaa
     1381 cttggagaag gcttccaatt cgcggtactg agccatgtcg agcttcaggg taccggccac
     1441 tttcttcata gacttgatct gagcgttacc ccccacacgc gataccgaga tacctacgtt
     1501 gatggccggg cgcacacctg cgttgaagag gttggcttcg aggaatatct gaccgtcggt
     1561 aatggaaatt acgttggtag gaatgtaggc agatacgtcg ccggcttgcg tttcgatgat
     1621 gggcaaagcc gtgagcgagc cgccgccttt cacgatgccg gcttttttca aagactccgg
     1681 caggtcgttc atgttgcggg caatgtcgtc attgttgatg actttggcag cacgctccag
     1741 caggcgggag tgcagataga acacgtcgcc gggataagcc tcgcgtccgg gcnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnncggaa gaactcgccg atggcagcgc cggcaaaagg
     1921 agcgtaaaac ttcagcggcg cgggatcggc agcagaagcc gaaacgatca cggtgtagtc
     1981 cattgcgccg tgctcttcca gcgttttggc tacgttggcg accgtagaag ccttttggcc
     2041 cgatgccaca tagatgcaat acaccggttc gccgcggtcg taaaactcct tctggttgat
     2101 gatggtgtca atggcgatgg ccgttttacc ggtctggcgg tcgccgatga tcaattcgcg
     2161 ctggccacga ccaatcggaa tcatcgcgtc aatggctttg atacccgttt ggagcggttc
     2221 gttcaccggc tggcggtaaa taacgccggg agctttgcgt tcgagcggca tttcatactt
     2281 ggcgcccgtg ataacgcctt tgccgtcaat gggaagcccc agcggattca ctacgcggcc
     2341 cacaaagccg tcgcctacgg aaatagaggc gatacgcccc gtgcggtgta cgcgcgagcc
     2401 ttctttgatg cccgtaccgg aacccatcaa caccacccct acgttgtctt cttcgaggtt
     2461 gaggacgatg gcttccacgc cggtttcttc aaaaactacc aattcgccgg cctgagcatt
     2521 gttgaggcca tacacgcggg cgataccgtc gcccacctgc aatacggtgc cgtattcttc
     2581 cagttcgctg gatgtgctga agccggagag ttgttgcttc agtatcgcgg agatttcatc
     2641 gggtttgaca tcaaccatga cgtatgttgt tttgaatatt tacgaatgaa attattaatg
     2701 agaaatgatc tgagaaacgt acgggttgtg aacgaattca cggcgcagcg tttccaattt
     2761 atgttgcaaa ctggcatcat acaccagatc gtccacttcc aaaatgaacc cgccgatcat
     2821 atcggcatcc accgaagtgc ggaactccgt gtgcgcgccg gtaatgcctt ccgacttgag
     2881 cttggcttcg atggcctgaa tggcgttgtc ctccaacttc acggcagaag tgaccttcac
     2941 cgtagagatg cctcgcagct ttttgaaaag cgtgatgaat tccttagcca cctcgcccat
     3001 gaacttctcg cggcctttat taaaaaggat gcgcaaaaac gccatggtga gtgggtcgaa
     3061 tttatcggcc agcatttttt ccatcacctc gcttttgcgt ttgtgctgaa tgacagggct
     3121 tttgataaaa agctgaaagt cgcgattttg catcagcgta tggaacgcct gcacatcttc
     3181 cagcacacga tctaatttcc tttgctctac ggccagatcg tggagagact tggcgtaacg
     3241 aacggctatc ctgtaagcgg acataaatgc ttgttataaa aatgaacggg caagccaaag
     3301 accggccctc aattaattca atttcatctc atccatcagg cggttcacga gctgctcctg
     3361 atcggcattg cctttcaggt tggcgcgcaa caggcgttcc gtgaggtcaa tggccatatt
     3421 gcccacttgg tttttcacct caatgatggc cgccattttc tgattttcaa tcagttcttt
     3481 ggcattttcc acaatcaaac gagcttcttc tttggctttt acacgcgctt cgttgatgat
     3541 ggcctctttc gtttcggcag cttcgcgcaa gattttcgtg cgttcctcct gagccaattt
     3601 gagcagggct tcattgtctg ccttcagttt ggacatctcg tcgcgggtgc gcttggcttc
     3661 atccaaagac gtttgaatgt cggtctcgcg ctgtttgagc gctttttgaa tgaccttgaa
     3721 cgccataggg ccgagaatgg cccaaagccc gatgaagatg accgaagtcc agaatatttg
     3781 tcccggatcg ggctggatga cggaaaactt agctaagaac aacatttgag acatagctat
     3841 aaaaaattgt aatgatggtt cttaaatagg gccggaccgg cagccaaccg ccccgatccg
     3901 accgtttttt cggttcgcag cgcgaattac ttcaacagac ctaccacgat ggcgaagagg
     3961 gcagcgcctt ctacgagggc cgccgtcagg atcatgttgt tacggatgtc gcctaccgct
     4021 tccggttgac gagcgatggc gtccatggcg cggctgccga taaggcctac gccgatgcct
     4081 gcacccaatg ctgcgattcc agctccaatt gctcccattg ttgagatatt taaaatggtt
     4141 aatatttcaa atttcggatt cctgatttcg gattcctgat ttccgattcc tgatttcatg
     4201 taggaatttt gaatcctgaa tcaggaatca ggaattcttc ctagtgatgc gcctcctcgt
     4261 gctcgtgatg tccttcatcc actgccgcgc cgatgtaaga agcggtcagg atagagaaga
     4321 taaatgcctg aaggaaagcc actaataatt caatcaggtt cataaaggca gtgaatgcac
     4381 cgccgatgac tgcacccact gttgcgccac cgatgctggt tccattttta ccaaagataa
     4441 aaatgaggcc gataaggctc aatatgatga tgtggcctgc cgaaatgttg gcaaacaaac
     4501 gaatcatcaa agagaagggc ttgatgaaca ggccgagaat ttctaccggc gtcaggatga
     4561 ttttcaccca ggcaggaata cccggcatcc agaagatgtg ttgccagtag tggccgttgc
     4621 cgttgaggtt ggtgaccaaa aaagccagca cagccagcac cagcgtcacg ccgaggttgc
     4681 ctgtgatgtt ggcgctgccg ggaaagaagg gaatcaggcc catcaggtta caaaacagga
     4741 tgaagaaaaa cagcgtcata atgaagggtt ggaagcgttc gtagtgcttc tcgccgatca
     4801 tcgggcgggt tacttcgtcg cggatgaaca tgaagatagg ctcgatgaag ccctgaatgc
     4861 ctttcggcgc tttgccttga ttgtttttat aacctcttgc tacgctcgaa aacagcagca
     4921 ccagcagtac cgctgccagc aacatggtag ccacgttctt ggtaatggaa aaatcgtaaa
     4981 aagacgtgat gccgccgccg agaataccac catcgagcgt gctgggcgaa tccaaacgat
     5041 attctttgcc ttcgtgcatt acataataca cttcctcttc ttgctccttg ccggctttcg
     5101 tcaccgcttc tttatggctt acaatgccct cgatatgtac ttcgcctgtg gggaaagaga
     5161 catccgatac cctgttgacg cggccgtgat tgagcacata gccgtcaata gctttgtgac
     5221 catgatcgaa cttgccggaa gacataaccg tccagccttg ccccggagcg tacaggaaac
     5281 aaggaagcgg aatgtgaata cctttccaaa cgtggaactc attggcatcg gcaatgtgat
     5341 tcattaccgc tcctacgggg tcgtattcgt cgtggccggc gccatgtttt tcttctgcga
     5401 gagccggagt ggcgacgcaa aaaagggcgg ctgcaaagag taagatgaaa cgcttgtata
     5461 tcattgcctg atgatcttct gtgatagctt gttgaaaacc ggcgcaaagg tagaaacttt
     5521 gtggaaaaaa aagaccaaat aaaatttttt atccgctttt gagtctcccg caacgcggtg
     5581 caaattgtta attccggctg ccgcacttga cagacagcct acaagcgcat acttttgtag
     5641 ggccttgagc gttattcagg cgcaactatc agaaaaagaa gccggtagag cggggacaat
     5701 cacgttctcg ttactttcgg caggtctcaa cgtctgaaca atagtttttt tcacccctgc
     5761 tgtcgggtac aaaacttcgg caccgggaaa aaatgcaaca aatgagaatt tttctttggt
     5821 tgatgtttct ccttcccgtt tcgggcttcg cacaagccga tttttcaggc atggccaaag
     5881 ctttgggcag cggcaatgca gatgcgctgg ggcaatactt cgatcaaaat gtcgaaatat
     5941 ccattatgga tcaggaggat gtatatgcca aaactcaggc catcggcgtg gtgaagcaat
     6001 tcttcagcca gaatgcgcct cgcagcttcc agcaaatgca tcagggcgcc tccaaaggca
     6061 gcgatgccca atattctatc ggcaacctga ccactgcatc cggcacattc cgcgtttatt
     6121 tgtacgctaa aagctccggc ggcaaattcc tcattcagga agtgcgcttc gataagggaa
     6181 gctgattatc tttgccggaa atcagcaata catggcgctc gcctggcgca tagcccgtag
     6241 atatttgttt tccaaacgca gcaccaatgc catccacatc atcacgggca ttgcagtgtt
     6301 cggtattgcc gtaggcgcag cggccctgct gctcgtgctt tccgtgttca acggtttcga
     6361 agacctcatc acgggcatgt acaacagctt caaccccgac ctcaaaatca cgcctgcaca
     6421 gggcaaaact tttcctgccg attcggcttt tctgtcgcgt atccgggcgg ttccgggtgt
     6481 gaaagacatt tcctgcacgc tcgaagaggt ggcttttttt gagtacaaaa acagccagga
     6541 tttcggcaca ttgaaagggg tggacgccga ttatactaag gtttccagcg tggacagcat
     6601 ggttcgggag ggtatttttc gtctccaaca ggaagatcgc tcttatgccg tactaggatt
     6661 gggtatgcgc aataagctca atgtcaacgt ggacgatgtc ttctcgcctc tggcggtcta
     6721 tatgcccaaa cgaaaggaaa ctgccgcatt ggagcaggct tttcgcaagc gattcattta
     6781 ccctgccggc acttttgtga ttcaacagga gtttgataat caatatatta tcaccaattt
     6841 ggaagctgcc cgcgacttgc tgggctacga ggaggaagtg agcgcgctgg aaatcaaagt
     6901 aaatgccggc gctgcacctc aaagtgttca gggggccgtt gctgcgctgt tgggaccgga
     6961 gtttgtggtc aaaaaccgct atcagcagga agagaccttc atgcgcctca tgcaggtgga
     7021 aaaatggatg agttttgcca ttgtgagcct gatgatgctg atggtgtcgt tcaacctcat
     7081 cggcgctctg tggatgatcg tcctggaaaa acaacccgat atttcgatca tcaaatctat
     7141 gggcggctcc gaccgcttgg tgcgcaatat cttcctgctg gaaggacttt tgctgtgcgt
     7201 tatcggtatc gcaaccggaa ttttgttggc cggaggcatc tatgtggctc aaaagcaatt
     7261 ggggcttgtt tccattcccg gcaatttggt catagattcc taccccatca gtatgcgctt
     7321 cggcgattta ccggtggtgg ccgttacagt actgggcatc ggcctgctgg cttccctgcc
     7381 gccggcactg cgcgccatgc gggtgcctgc gctgatgcgg gaggagtaaa ttttattcca
     7441 aatataattt gtctgacttc gccctctcaa ccttacacca ctatatatgg tacccgtaaa
     7501 gatagaacac atcctggccg acctgtttga aaaatgggcc ggcgagagcg cttatttggt
     7561 gttgcctttg gcgccctccg gctcgcaacg cgtgtattac cgcctgcaag ggccgacgaa
     7621 aaccgccatc ggggcctaca atcccaatcg caaagaaaat caggcattta tctccttcag
     7681 ccggcatttc aaagctcgcc aactgcccgt gccggagata tacgccgaag acctcgatca
     7741 ggacgtgtat cttcaggaag acctgggttc caccactttg tacagttatc tgctgcaaaa
     7801 gggcgaatat tttcccgatt atttggtgca gatttaccga aaggcggtgg agcaattggc
     7861 ccggctgcaa attgagggcg gcaaagactt ggactacagt gtgtgttacc cccgcgacgc
     7921 tttcgaccgg cagtctatgg tatgggattt caacaccttc aaatattact tcctccggct
     7981 ggcttatgtg ccgtttgacg aacaggcttt ggaggccgat tttcagcgct tggccgactt
     8041 cctcttgcag gccgactgca atcacttcat gttccgggat ttccagaccc gcaacatcat
     8101 gattaagagc ggagagcctt attttattga ttaccagggc ggtcgccggg gagctttgca
     8161 gtatgacctg gcctcgctgc tgtatcaggc caaagctggt atgcctgagg acatccggca
     8221 ggaattgttg gaacactaca tcaaaacagc cgggcaattc atccacattg accgcgaaca
     8281 attcatccaa tattatcacg gctatgtgct cattcggagc attcagacct tcggcactta
     8341 cgggtttcgg gggctgtttg aacggaagga gtactttttg gaaagcattc ccttcgcgct
     8401 ccgcaatgtg cgctggatat tggaaaacgc ccatctgccc gttgaaatgc ccgaactgac
     8461 ccgcgccatg taccacctca tcgaatcgaa gcgcttcgag ccgtttgata aaataaaggg
     8521 ggcttccagt ttactcaccg tgacgatcaa cagcttttct tacaaatcag gcggcatccc
     8581 cgaagacccg cacggaaatg gcggcggttt tgtgttcgat tgcaggtgca tcaacaatcc
     8641 ggggaggtac gaaccctaca aaaaactgac gggccgcgat gagccggtca tcaacttcct
     8701 caagcaccac agccacatgg gagattatct gaacagcgtt tttcacttgg tgaatgatgc
     8761 ggtggaagac tacatcgagc gcagttttac gcacctcagc gtcaatttcg gttgcaccgg
     8821 aggccagcac cgctctgtgt atgccgccga tgcgctggcc cggcatttga aagataaata
     8881 cggcgtacgg gtggagttgc gccatataga gcaggagcgg aagaactggc agaattagtg
     8941 ccccattccg gtttgattgc tcaccggcct ggcatacatg ctcaggaagc agcggagagc
     9001 ctcggcgtcc atgccggctg tacgttcctc catgcgatgc acaaaagcga ctttttcctc
     9061 cggggnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtttgc gaaagcagat cgtgtgagat
     9121 gtaattggat ggccaaagcc ggtaattggc aacaatggcc cggtccaaaa cctgcgccaa
     9181 cgctttgatt tgatgattga gtcccgaaat ggccgctatc tcatccaact cagcatccaa
     9241 aggcgtgccc gcatggaaat ggatgcgttt tttttgtccc cgtatgcccg tcatgatgtg
     9301 ccggaaatct tcctgtggct ctttggtata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcca
     9481 tgccgatcat tttcagaacg ccggggttcg tggcatcgtt gccgtctttg gcgcggcctt
     9541 cccgttgggc aatccatacc gaacggcggt cttccaacat cagatggcgg atgtatgcag
     9601 tcagttcctg cgagctttct aagagttcgc gcggcggcag gttgcggcgc actaaaaaat
     9661 tgcggttgat tttggcaaat gtgagcaaaa acggtagttt caccaagttg tcgccgatgg
     9721 ccgaagcggt catggtaagc ccctgctcaa gcaggataaa gttgagcagc gccgtgtcga
     9781 gcacgatgtc ccggtggttg gaaacatata aataaggcgt atcgggcagc agttggtcaa
     9841 agccggaatg tgtgaggccg gccgagcttt ctgcgaggat gcgaaggacg gccggtctga
     9901 taacctgtgc gtgaaagtct tgagtggcac gaatattatc caaaatttgg aggcgtcctt
     9961 cgggagccag ttcgnnnnnn nnnnnnnnnn nnnnnnnnnn nccgtctgca tcatggggtc
    10021 atgggcaata gagcgcaggg cggcgtgtac ttcgtcgtcc cggaaagcgc gaatagagtc
    10081 gaaagcttcg ggcattgtta caattattgg tgaacgctgc tgcgaaggta cgcaatcacc
    10141 acgtcaatcc cggcatggcg cggggcacct gttgcatttc ggccaggatg tagtccatgt
    10201 gttccgtat
//
LOCUS       DAORLF010000131        10171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3540_length_10171_cov_1.888296, whole genome shotgun sequence.
ACCESSION   DAORLF010000131 DAORLF010000000
VERSION     DAORLF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_10171_cov_1.888296"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK198_11815"
     CDS             complement(<1..411)
                     /locus_tag="PK198_11815"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRF39463.1"
                     /translation="MKNIIMLLAALSVAACSSGAKLGKAGAGEVHITGAMRDAMWKGE
                     IYGKINMDTISDKTHLFGLGPVEYLSGELLIADGRCYRATVVSAEEMKVEEVWTVKAP
                     FFVRAKVPAWKESALPDSIVSLPQLESYLNQITRK"
     gene            553..1776
                     /locus_tag="PK198_11820"
     CDS             553..1776
                     /locus_tag="PK198_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF39464.1"
                     /translation="MPDILIIGGGAAGFFAAIRCAELYPDAQVALLERGKELLGKVKI
                     SGGGRCNVTHACFVPKDLIKNYPRGSKELLGPFTRFACGDTMEWFESRGVALKIEDDG
                     RVFPESDSSQSIIDCLLDAARQAGVRILLHHRIDDILPPAQAGEPWQINTSEGPLSAD
                     RILIATGSNTRIWEQLAALGHSIVPPVPSLFTFNIKDPALAALPGLSVPQADVSIPGT
                     DLAASGPLLITHWGLSGPAILRLSAWGARILAGKQYRFNLRVNWTGLSIPDIEEELEW
                     LRQQYGKRQAAAHPQFDIPTRLWRALCLSAWGERQLDSKWGDLNKGMRDKLINRLAAS
                     EYSVNGKSTFKEEFVTAGGVDLREINFKTFESKLHPGLFFAGEVLDIDAITGGFNFQA
                     AWTGGWIAGEAMGAF"
     assembly_gap    1830..1852
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1950..2393
                     /locus_tag="PK198_11825"
     CDS             1950..2393
                     /locus_tag="PK198_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39465.1"
                     /translation="MKTILLLAGLLLVNISFAGIVPQHIQQAFEKDHPEAEAAFWEIT
                     KEGYAATFQAEEGLKKAIYSEEGGWLETRTRILLRDIPADVQREIRKEIGYTKTTYIG
                     KVLSPEGRFYRIESETAEAVVVRIFDENGRLISENEFAFSTNSKA"
     gene            2398..>2922
                     /locus_tag="PK198_11830"
     CDS             2398..>2922
                     /locus_tag="PK198_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF39466.1"
                     /translation="MEYRQFGKTDLKVSVVGFGAWAIGGPAQVGDMAIGWGPSNDAES
                     VGAIERAIELGINFFDTADFYGLGHSEVLLGRMIGNRQDVIVASKTGQKIGDNGNVAI
                     DYSKKHILRTCEESLSRLRRDAIDFHHLHVARMQHLEQGECIEAMEQLVQEGKVRYWG
                     ISLNTFAPEPEADFF"
     assembly_gap    2923..2939
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2940..3399
                     /locus_tag="PK198_11835"
     CDS             <2940..3399
                     /locus_tag="PK198_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF39467.1"
                     /translation="QIGHGFQLVFNVINQRAAGVIERARNAGYGVIARMPLQFGLLSG
                     KFAPQTTFHPEDHRAKRLRPEVLERALSDLQPFAEMAKSKGVTQAQLALSFAAHYPGI
                     TTIIPGIRTPYQAELNAEALLALNADDMAQLSLLYEEHLADLMSFLETAG"
     gene            complement(3503..>4436)
                     /locus_tag="PK198_11840"
     CDS             complement(3503..>4436)
                     /locus_tag="PK198_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39468.1"
                     /translation="CHSCPVASSITEFKLSGDSAQLRSSMPFTFTGTYRLQDRDLTIR
                     LDGGGTIQKRIAFFSPDSLRFEGDTSLFYRYDYEYRFPPVFSLIGIRTGLTVGNELDQ
                     RIERAGVRVHGGIALLKNEKGETVWSNGEYFKPLSDIREESCFDCWPTPYIGVMIGEG
                     IKLRDLAQLLGLLQRADNKHLVRLILYTGGPGHFEYFEDNIELWYEDAVIFMQNAERI
                     KNYPPPPPPPPPPYQKTREYYLSQGAELIQINTPEEIDELRNNLSIKGKYLIGIGLDL
                     SIEDYIRIKELTQVLRKYKGYRIRTELRPDEQYK"
     assembly_gap    4437..4527
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4736..>4894
                     /locus_tag="PK198_11845"
     CDS             4736..>4894
                     /locus_tag="PK198_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF39469.1"
                     /translation="MLNIRNVAIIAHVDHGKTTLVDKMLLAGRLFKEHETPGELILDN
                     NDLERERGI"
     assembly_gap    4895..4952
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4953..6560
                     /gene="typA"
                     /locus_tag="PK198_11850"
     CDS             <4953..6560
                     /gene="typA"
                     /locus_tag="PK198_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRF39470.1"
                     /translation="IDTPGHADFGGEVERVLNMADGVLLLVDAFEGPMPQTRFVLQKA
                     IELGLKPIVVINKVDKDNCTPDEAHEAVFDLMFSLGATEQQLDFPVLFGSAKQNWMGY
                     DWRNPSNDITPLLDTIVEHIPPPVIAEGSTQMLITSLDYSAYIGRIAIGRLYRGSLKD
                     GQQVALIKKDGAVVKSKIRGLYVFEGFEKIKAERVEAGEICAILGVDGFEIGDTIADP
                     ENPEGMTRIAVDEPTISMVFTINDSPFFGKEGKFVTSRHLKERLERELEKNLALRVQP
                     TAQAETFNVFGRGVLHLSVLIETMRREGYELQIGQPQVILKDVDGQVHEPIEEMTIDL
                     PDNMSGTAIDMVTRRRGVLLSMTPKNERVILQLEIPSRGIIGLRSQMLTATQGEAIMT
                     HRFKEFQPYKGDVPGRTNGSLICMETGTAIPYSIFNLQDRGSFFVDAGEEIYEGQVVG
                     ENTRAGDLIVNLTKTKKLTNMRASGSDEKVRIIPPIKFSLEEALEYIQEDEYVEVTPK
                     SIRLRKILLKEHERKRAKNSALSSQVG"
     assembly_gap    6693..6902
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6903..7112)
                     /locus_tag="PK198_11855"
     CDS             complement(<6903..7112)
                     /locus_tag="PK198_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF39471.1"
                     /translation="MPLPDRSAATQLLEHHVQDAYQRYHARMVATAMEGYARHFGEDE
                     DLWYLTGYLHDLDFEQFPEQHPAVSL"
     gene            complement(7136..>7711)
                     /locus_tag="PK198_11860"
     CDS             complement(7136..>7711)
                     /locus_tag="PK198_11860"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRF39472.1"
                     /translation="VDKQSGYLKIDRPQKFSNIVPALYGFIPQTYCKAQVAEFCMQAT
                     GRANIVGDDDPLDICVLTEREVTHGNIIVNATPIGGFRMIDGGEADDKIIAVLTKDEV
                     YGQWHDISELPASIINRLRHYFLTYKQLPGEPINCEITHVYGREEALEVIRRSQADYN
                     EAYGDLEGVLSATLSEAFHFSRRQQDILSGL"
     assembly_gap    7712..7753
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8090..8172
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8173..8869)
                     /locus_tag="PK198_11865"
     CDS             complement(<8173..8869)
                     /locus_tag="PK198_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF39473.1"
                     /translation="MKKMHLWFFAASCALLAISAQAQTPAVSAGRIERHEQFPSQFVS
                     ARNVDVWLPEGYSPRKKYAVLYMHDGQMLFDGAGTWNKQEWQVDEIAGKLLAEGKIRD
                     CIVVGIWNGGQGRHTEYFPQKPFEQLSAQEKELVFSAVRSNGQQVFNSDLLQSDKYLK
                     FLTTELKPFIDKQYATRKDRKNTFIAGSSMGGLISMYAICEYPKVFGGAACISTHWPG
                     IFAIENNPVPGAFL"
     gene            complement(8926..9603)
                     /locus_tag="PK198_11870"
     CDS             complement(8926..9603)
                     /locus_tag="PK198_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39474.1"
                     /translation="MPYLQPTYHSKLYRDFRSIDASAWRDIIHFFERRETDIRALDFD
                     EYFELLYAYVNALFEVGAYQKHLVMVDEVIEAAICHNIHTYKQEDIFKKSLFRKAASL
                     YNTSDYHHAEHILRELVRMDPDYRDAVSFLKKCFRSIHFRSLQVFRAAGILFFLTAAL
                     VISIEVLFVRPFYARYADWVETVRMLLFGAGLGALTLGELWHRWLAHRRIEAFVADNR
                     KRKTSMQ"
     gene            complement(9603..>10171)
                     /locus_tag="PK198_11875"
     CDS             complement(9603..>10171)
                     /locus_tag="PK198_11875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRF39475.1"
                     /translation="NVRLNPHGNGTHTECVGHIAPEHFSLNDCLTNFHFIAKLHSVLP
                     QMQPDGDRVVMREQLEDTLMPGEAEAYILRTLPNATDKCRRHYSGTNPPYLHPDAVRW
                     LREAGVKHLLLDLPSVDREEDGGQLAAHKTFWNFPQAPRIDATITELIFVNNFIPDGL
                     YLLNLQITSIQLDASPSKPVLYALELLN"
BASE COUNT         2264 a         2656 c         2557 g         2170 t
ORIGIN      
        1 cttccgggtg atctgattca gataggattc caactgcggc aggctgacga tgctgtcggg
       61 cagcgccgat tccttccacg cgggcacctt ggcgcgaaca aaaaaaggcg ctttcaccgt
      121 ccatacttcc tctactttca tctcctcagc cgacacgacg gtagctcggt agcagcgccc
      181 atcggcaatg agcaactcgc cggagagata ttccaccggc cccaatccga acaaatgcgt
      241 tttgtcggaa atggtgtcca tattgatttt gccatagatt tcgcccttcc acatagcatc
      301 gcgcatagcg ccggtgatat gtacttctcc ggccccggcc ttcccgagtt tggcccctga
      361 gctacaagct gctaccgaaa gcgcggccaa cagcatgatg atgtttttca tattgtccac
      421 ttttcaggcg gcaaacttat gacaaaaaaa cggctacctt tgcccgccgg agcgagaaat
      481 cacaagcccg tgcgaggttc tacctcgtgc ggattatccg gcgggttcta cccgcccacc
      541 aaaacataaa taatgcccga catcctcatc atcggcggcg gcgccgcagg ctttttcgca
      601 gccattcgct gcgccgaatt gtaccccgac gcacaagtcg cgctcctcga acgcggcaaa
      661 gaactcctcg gcaaagtcaa aatttccggc ggaggtcgct gcaacgtcac ccacgcctgc
      721 tttgtaccca aagacctcat caaaaactac ccgcgcggca gcaaagaact cctcggcccc
      781 ttcacccgct tcgcctgcgg cgatacgatg gagtggtttg aatcgcgcgg cgtagccctc
      841 aaaattgaag acgacggccg cgtcttcccg gagtccgatt cctctcaatc catcatagac
      901 tgcctgctcg acgccgcccg ccaggccggc gttcgcatcc tcctccacca tcgcattgac
      961 gacatcctgc cgcccgccca agccggcgaa ccctggcaga tcaacacctc cgaagggccg
     1021 ctttccgcag atcggatact catagccacc ggcagcaaca cccgcatctg ggagcaattg
     1081 gccgcattgg gccacagcat cgtgccaccc gtgccttcgc tttttacctt caacatcaaa
     1141 gaccccgccc tcgccgctct gcccggtctc tcggtaccgc aagccgacgt atccattccc
     1201 ggcaccgacc tcgccgcctc cggcccgctg ctcatcaccc attggggact cagcggtccg
     1261 gccatcctgc gcctctccgc ctggggcgcc cgcatcctcg caggcaagca ataccggttc
     1321 aacttgcgcg tcaactggac cggcctttcc attcccgaca tcgaagaaga actcgaatgg
     1381 ctgcgacagc aatacggcaa acgacaggcc gctgcgcacc cgcaattcga catccccacc
     1441 cgcttgtggc gcgcactttg cctctctgcc tggggcgaaa ggcagttgga cagcaaatgg
     1501 ggcgatctca ataaaggcat gcgcgacaag ctgatcaaca ggctcgccgc ttccgaatac
     1561 agcgtcaacg gcaaaagcac tttcaaagaa gaattcgtca ctgccggcgg cgttgacctc
     1621 cgcgaaatca atttcaaaac ctttgagagc aaactgcatc ccggcctgtt tttcgccggc
     1681 gaagtgctcg acattgacgc catcaccgga ggattcaact ttcaggcagc ctggaccggc
     1741 ggctggattg ccggagaggc gatgggagca ttctgaaatg gaagattgga tggctcttct
     1801 tcaggaaaaa gggccgataa atagtaatan nnnnnnnnnn nnnnnnnnnn nngcgacaac
     1861 gatttgggcc tgaaaatgtt actaccctgt ttctgcgccg gatgcgccgg agcctcgaca
     1921 cctgacattg atcaaaacaa acagaaatca tgaaaaccat tctgctgctg gccggcttgc
     1981 tgctcgtcaa tatctccttt gccggcattg tacctcaaca tattcaacag gcttttgaaa
     2041 aagatcatcc cgaagccgaa gccgcgtttt gggaaattac caaagaagga tatgccgcta
     2101 cttttcaggc ggaggaagga ctgaaaaagg cgatttattc ggaagagggc ggctggctgg
     2161 aaacccgtac acgcatttta ttgcgagaca ttcccgccga cgtgcagcgc gaaatccgca
     2221 aagaaatcgg ctacaccaaa actacttaca tcggcaaagt actgtcgccg gaaggtcgct
     2281 tttaccgcat cgaatccgaa acggcagagg ctgtggtcgt gcggattttt gacgaaaacg
     2341 gccggctgat ctctgaaaat gaatttgctt tcagtaccaa cagcaaagca taaaacaatg
     2401 gaatacaggc aatttggaaa aaccgacttg aaagtaagcg tcgtaggctt cggggcctgg
     2461 gccatcggtg gtccggcgca ggtgggcgat atggccatcg gatgggggcc gagcaacgat
     2521 gcggaatccg tcggggccat cgaacgcgct atcgaattgg gaatcaactt tttcgatacc
     2581 gccgattttt acggactggg gcattccgag gtattgctcg gcaggatgat cggcaaccgg
     2641 caggatgtca ttgtcgcttc caaaaccggc cagaaaatcg gcgacaacgg caacgtcgct
     2701 attgattatt ccaaaaaaca catcctccga acctgcgagg aaagcctgag ccgattgcgc
     2761 cgcgacgcca ttgattttca ccacctgcat gtggcgcgta tgcagcattt ggagcagggc
     2821 gagtgcatcg aggcgatgga gcagttggtg caagaaggca aggtgcgtta ctggggcatt
     2881 tcgctcaata ctttcgcgcc cgaaccagag gccgatttct ttnnnnnnnn nnnnnnnnnc
     2941 cagatcggac acgggtttca gcttgttttc aatgtcatca accaacgcgc cgcaggcgtc
     3001 atcgagcgcg cccgcaatgc cggctatggc gtcattgctc gcatgcccct gcaatttggc
     3061 ctgctcagcg gcaaattcgc gccccaaacc accttccacc cggaagacca ccgcgccaaa
     3121 cgcctccgcc cggaagtgct ggaacgtgct ttatccgacc tgcaaccttt tgcagaaatg
     3181 gctaaaagca agggtgttac gcaggcgcaa ctcgcgttga gctttgcggc tcattatccc
     3241 ggcatcacca ccatcatacc cggcattcgc acgccttacc aagccgaact caatgccgag
     3301 gccctccttg cgctcaacgc cgatgacatg gcacagttga gcttgctgta cgaggagcat
     3361 ctcgcggacc tgatgagttt tttggaaaca gcgggttagg gagcgtttga gactgtgaga
     3421 gggggaggct gtgagagggc ttttacataa aagctccgtg aaggctccgt gtcctccgtg
     3481 cctccgtggt gattcaaaaa attcacttgt actgttcatc cggcctcagt tcggtgcgaa
     3541 tgcggtatcc tttgtatttt cgtaagactt gcgttagttc cttgatgcga atatagtctt
     3601 caatagagag gtcgagacct atgccgatca ggtatttccc ttttatactc aaattattcc
     3661 tcaattcatc aatttcttca ggtgtattga tttggattag ctctgctccc tgtgataggt
     3721 agtattcgcg agttttctgg tatggcggcg gcggtggcgg cggtggagga ggatagtttt
     3781 tgattctttc tgcattttgc ataaatatca cagcatcctc ataccataac tctatgttgt
     3841 cttcgaagta ttcaaaatgc cccggcccgc cggtgtacaa aatcaggcgg acaagatgct
     3901 tattatctgc cctttgtagc aatcccaaca gttgagccaa gtcgcgcagt tttattccct
     3961 ccccgatcat caccccaatg taaggagtcg gccagcagtc aaaacacgat tcctcccgga
     4021 tatcgctcaa gggtttgaag tattccccgt tgctccagac agtttctcct ttttcattct
     4081 ttaacaaggc gataccgcca tgcacccgca cgccggcgcg ctcaatacgc tggtccaact
     4141 cattaccgac tgtcaaaccc gtgcgaatac caatcagaga aaagacaggc ggaaacctgt
     4201 attcataatc atacctgtaa aaaaggctcg tatctccttc aaagcgcagg ctgtccggtg
     4261 aaaaaaaggc gatccgtttt tgaatcgtac cgccgccatc caatcgaatg gtcaaatccc
     4321 gatcctgcag ccggtaagta cctgtaaaag taaagggcat tgatgacctc aattgcgcac
     4381 tatcccctga taacttgaat tctgttatag aagaggcaac agggcagcta tggcacnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnagc caggcaatag taggaaaagg tttttcatgc
     4561 ggtcgtttgt tgcttttcaa aatgaaagat gcaaagtgga gcgaaagagt tgggatgccc
     4621 ggcaattttt cagtaagttg aagcgcaggt ataagtttta caaaagaata aaatttggaa
     4681 tcccttcatc tgccgattta tccctacctt tgcgccctca tttaaaaaag catccatgct
     4741 gaacattaga aacgtagcca tcatcgcaca cgttgaccac ggaaaaacta ccttagtgga
     4801 taaaatgctg ctggccggcc ggctcttcaa agaacatgaa actcccggcg aacttatttt
     4861 ggacaacaac gacctcgaac gcgaacgggg catcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatagatac tccgggtcac gcagacttcg
     4981 gcggcgaagt agagcgcgta ctcaacatgg ccgacggcgt gcttctgctc gtggatgcct
     5041 ttgaaggtcc catgccgcaa acccgctttg tgttgcaaaa ggccatcgaa ctgggactca
     5101 agcccatcgt ggtcatcaac aaagtggata aggacaactg tacgcccgac gaagcccacg
     5161 aagccgtttt cgacctcatg ttcagcctcg gagctaccga acaacagctc gattttccgg
     5221 tgctgttcgg ctccgccaaa caaaattgga tgggctacga ctggcgcaat ccttccaacg
     5281 acatcacccc gctgttggac accatcgtgg agcacattcc accgccggtg atcgcagagg
     5341 gcagcaccca gatgctcatt acttctttgg actactcggc ctacatcggt cgcatcgcca
     5401 tcggccggct ctatcgcggc agtctcaaag acgggcaaca ggtagcgctc atcaaaaaag
     5461 acggcgctgt ggtaaagagc aaaattcgcg gcctgtatgt gtttgaaggt tttgaaaaaa
     5521 tcaaggcaga gcgtgtagaa gcgggcgaaa tttgcgccat cctcggcgtg gacggctttg
     5581 aaatcggcga caccatagcc gatcctgaaa acccggaagg catgacccgc atcgctgtgg
     5641 acgagcctac catcagtatg gtgtttacca tcaacgactc tccgtttttc ggcaaagagg
     5701 gcaagtttgt cacctcgcgc cacctcaaag aacgcttgga gcgcgagttg gaaaagaacc
     5761 tcgctctgcg tgtacaaccc accgctcagg ccgaaacttt caacgtgttc ggtcgtggcg
     5821 tattgcacct ttctgtgctc atcgagacca tgcgccgcga gggctatgag ttgcaaatcg
     5881 gtcagcctca ggtgattctg aaagatgtgg acggccaggt acacgaaccc atcgaagaaa
     5941 tgaccattga cctgccggac aacatgtcgg gtacggccat tgatatggtg acgcgccgca
     6001 ggggcgtgct gctgagcatg acgcccaaaa acgagcgcgt gattctccaa ttggaaatcc
     6061 cgtcgcgcgg catcatcggt ctgcgcagcc aaatgctcac cgccactcag ggcgaggcca
     6121 ttatgacgca ccgtttcaaa gaattccagc cgtacaaagg tgacgtaccg ggtcgtacca
     6181 atggctcgct catctgcatg gaaaccggta ctgctattcc ttacagtatt ttcaaccttc
     6241 aggatcgcgg cagttttttt gtggatgcag gcgaagaaat ttacgaaggt caggtggtag
     6301 gcgaaaatac gcgtgccggc gacctcatcg tgaacctgac gaagaccaaa aagctcacca
     6361 acatgcgggc ttccggctcc gacgagaagg tgcgcatcat tccgcccatc aagttttcgc
     6421 tggaagaagc gctggagtac attcaggaag atgaatacgt ggaagtgacg ccgaagtcca
     6481 tccgcctgcg gaagattttg ctgaaagaac acgagcgcaa gcgcgcgaag aactcggcct
     6541 tgtcgtcgca agtgggttaa tccagcttcc caaaaaactg tatcaagtgg acgacgtgct
     6601 cttcaatggg cacgcccaga tgctcgcatc ccaagaggat ggtgctgcgg tctattttgg
     6661 cggcgaaggc tttttccttc aggcggctca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nncagtgaaa cggcgggatg ctgttccgga aactgctcga aatccagatc gtgcaggtac
     6961 ccggtgaggt accacaaatc ttcgtcctct ccgaaatgcc gggcataacc ttccatcgcc
     7021 gtcgctacca tccgtgcgtg gtagcgctga taggcgtcct gaacgtgatg ttcaagaagt
     7081 tgtgtagctg ctgatctgtc gggaagtggc atggtgtatc aattttaatt tctactcaca
     7141 acccactcaa aatatcctgt tgccggcggc tgaagtgaaa cgcttcggac agggtagccg
     7201 acagcacgcc ttcgagatcg ccgtaagcct cgttgtaatc ggcctgactg cggcggatga
     7261 cttctaatgc ctcctcgcgg ccatacacat gcgtgatttc gcagttgatc ggttcgccgg
     7321 gcaattgctt gtacgtcagg aagtaatgtc gcaggcgatt gatgatgctg gccggcagtt
     7381 cgctgatgtc gtgccattgg ccatacacct cgtctttggt cagcacggcg atgattttat
     7441 cgtcggcctc accgccgtca atcattcgga agccgccgat gggcgtagca ttcacgatga
     7501 tgttgccgtg tgtgacctcg cgctcggtga gtacgcaaat atcgagcgga tcgtcgtcgc
     7561 ccacgatgtt ggctctgccg gtggcctgca tgcaaaattc ggctacctgc gccttacagt
     7621 aggtttgcgg aataaagccg tacaaagccg gcacgatgtt ggagaacttt tgcgggcggt
     7681 caattttcag gtagccgctt tgtttgtcca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnngtcactt ttcgcggcgc atccggcccg atggggatgc cgtgccaggg
     7801 gtgcgaggtg tatcgcagcc cgatcatttt ccacagtttg ctaaaggatt cctgactcat
     7861 tattcattgg ttgtttgagg cgacaaaggt aagggatttg gtcggctctg cgccgaattg
     7921 cgcatcgcat aaaaatttca cctccccagc aaaaacacca gtggaatatg cagccgcccg
     7981 gcccatgcgc gctccgagtg gtcttcgccg ggaaaagcct gcgacaacag atttttgacg
     8041 ccgtaacctc ctgctttcag gatcacatcg gcttgcgcct gatacggcgn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nngcaaaaat gcgcccggca ccggattgtt ttcgatggcg aaaatgcccg
     8221 gccaatgggt ggaaatacag gccgctccgc cgaacacttt cgggtattcg cagatagcgt
     8281 acatggagat gaggccgccc atgctgctgc cggcaatgaa ggtgtttttg cgatctttgc
     8341 gcgtggcata ttgcttgtca ataaagggtt ttagctctgt tgtcagaaat ttcaggtact
     8401 tgtcggattg cagcaggtcg ctgttgaaaa cctgctgacc gttgctcctg actgccgaaa
     8461 acaccagctc tttttcctga gcggagagtt gttcaaaagg cttctgcggg aaatactcgg
     8521 tatgccgtcc ctgcccgccg ttccagattc cgacgacgat gcaatcgcgt attttacctt
     8581 ccgcgaggag tttgcccgct atttcgtcca cctgccattc ctgtttgttc caggtgcccg
     8641 cgccgtcgaa gagcatctgc ccgtcgtgca tgtacagtac ggcgtacttt ttgcggggcg
     8701 agtagccttc cggcaaccag acgtccacat ttcgggcaga gacgaattgt gagggaaact
     8761 gctcgtggcg ctctatccgg ccggcagaga cggcgggcgt ttgggcttgc gccgaaatag
     8821 cgaggagggc gcatgatgct gcgaaaaacc aaaggtgcat ttttttcata cggagagcgc
     8881 ttgaagtgag ccgtaaagtt cgcacaaaat acggcaaatt catagctatt gcatgctcgt
     8941 tttgcgcttc cggttgtcgg ccacgaatgc ctctatgcgg cgatgcgcca gccagcgatg
     9001 ccaaagctcg cccaatgtga gcgcgccgag gccggccccg aacagcagca tacgcacggt
     9061 ttctacccag tcggcatatc gggcgtagaa aggccgtacg aacaacactt cgatacttat
     9121 gactaaggcc gccgtcagaa aaaacaatat gcccgctgcc cgaaacacct gcaaagagcg
     9181 gaagtgtatg cttcggaagc actttttgag gaaagatacg gcatcgcggt agtcggggtc
     9241 catgcgcacc aattcgcgca gaatatgttc ggcgtggtgg tagtcggagg tgttgtacag
     9301 agaagcggct ttgcggaaaa gggatttttt gaaaatgtct tcctgtttgt aggtgtggat
     9361 gttgtggcat atcgccgcct caatgacttc gtccaccatg accaagtgtt tttggtaggc
     9421 gcccacctca aacaaggcgt tgacataagc atacagcagc tcgaagtatt cgtcgaaatc
     9481 aagagcgcgg atgtccgttt cgcggcgctc aaaaaagtgg atgatgtctc gccaggcgga
     9541 ggcatcaatg ctcctgaaat cgcggtagag cttggaatga tatgtcggtt gtaagtacgg
     9601 catcaattca acaattctaa agcatacaag acaggtttgg acggtgaagc atccaattgg
     9661 atggaagtaa tttgcaagtt gagcaagtat aatccatccg ggatgaaatt gtttacaaaa
     9721 ataagctctg ttatcgtagc atctatgcgc ggcgcctgcg gaaaattcca aaaagttttg
     9781 tgcgcagcca attgcccccc gtcttcctct cggtccaccg aaggcaaatc gagcaaaagg
     9841 tgcttcacgc cggcctcgcg cagccaacgc accgcatcag gatgcaggta aggcgggttg
     9901 gttcctgagt aatgacggcg gcatttatcg gttgcgttgg gcagggtgcg caggatgtag
     9961 gcttccgcct cgccgggcat cagggtgtct tccaattgct cgcgcatgac cacgcggtcg
    10021 ccgtcgggct gcatttgcgg caagacgctg tgcagtttgg ctataaaatg gaaattggtg
    10081 aggcagtcat tgagcgaaaa atgttcgggc gctatgtggc ccacacattc ggtgtgcgtg
    10141 ccgttgccgt gcggattgag gcggacgttg a
//
LOCUS       DAORLF010000132        10051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3589_length_10051_cov_2.360344, whole genome shotgun sequence.
ACCESSION   DAORLF010000132 DAORLF010000000
VERSION     DAORLF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_10051_cov_2.360344"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK198_11880"
     CDS             complement(<1..1287)
                     /locus_tag="PK198_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF39476.1"
                     /translation="MKRKIILPAILAFMLVFSNSCNDWLDINFDPSNPQVAEGFALLP
                     PMLANMTRGETFDTRYVGQYIQNWNWIAANNVWDQHGYAPGSDAAGEKWRQHYWAIGK
                     NIELMIEQATLKSQWDYVGVGKSIRAWSWQTSTDVYGEMILTQMFEPNRYVFDYDTQD
                     KIYAEAERIALDAVADLAKTDGGVSQASLARGDLVYAGDRSKWTKFTYGVLARLANHV
                     SNKASYNPDKVIEYCDKALASNADNFDVPHAGTSSADGNFYGPTRNNLRSYRQSAFIL
                     SLLDGTVFSGVQDPRLPKMLSASPDGVYRGVTPTAGDPTNTTGNMARIPLLWDVSPTV
                     TVAAQIPGKYIFTDKAKHSLMTYSEIQFIKAEAAFKKGNKTLAYDAFRKGIIAHMDYA
                     GVTAANRDAYMASAAVPQDANALTLSQIMLQKYVSLW"
     gene            complement(1309..>2038)
                     /locus_tag="PK198_11885"
     CDS             complement(1309..>2038)
                     /locus_tag="PK198_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39477.1"
                     /translation="GNRYYPGLHDRGAGSATAIGGFSYLRNSKGQILINPTTGFPIAN
                     SNFLPIGDRNPDFTIGLVNDFRIMKNLTVSFLLDIRKGGDIFNGNELFLVRNGLSTRT
                     LNRDEAFIFAEGNSVLRDGNEETDNPTANNKLITPSANELFYTTALLPEDFVERDINW
                     LRLRDVTIGYNLTGKWIGPNQVIKELSVFVNGTDLLLITNYTGADPYVSTTNPATGGA
                     GGFGMDFGKISLPRTFSIGLSASF"
     assembly_gap    2039..2052
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2053..>3904)
                     /locus_tag="PK198_11890"
     CDS             complement(<2053..>3904)
                     /locus_tag="PK198_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39478.1"
                     /translation="LNQHNLYSDGVGINGNVNNNRDEAGSRLAELNPNDIESVTVLKG
                     PEAAALYGNEGANGVIVITTNKGKAGIGRLSYKTRFATSELYLFPEIQQVYGRGRNGA
                     VEVNDPDFFGPKYAEGTTFYDNIGDFFQRGSNMRHDLSFEGGAEKLTYRLSAGYLKTN
                     GVIPTNTYDQVNAALSSEAKLLPWLKAFTRFSFSKNENILPPGGAEGYLTAALRYPSD
                     QLMSDYLTKEGTRRLTLANATPATDNANPYFNVYKNARSERTDRTIGNITLDATITPW
                     WTVTGRFGADVYATDANRFFHPESNIGFPRAGWTENYTDIGRFLTTTMFTTLQKTQGN
                     LRGSLIVGMATNDRRNETSSLYGEKFYLPDFNSINNTDPTTQRNRTILTRTRLAGAFA
                     KGEINYQNWLIVNVTGRNDWSSTLPVENQSYFYPSAGLTYVFSDMPSLQGKLGFLDFG
                     KLRASYAQVGNPATPYRIRARLVPQTTTGGGFAYDFFGDNTNLKPEKVESFEIGTDLG
                     FFKGRVQLDVAWFSKTIKDQIVTQRLSYGTGFIFGLLNGGELNTKGIEIQLGLTPVKK
                     DNFTWRLNTNFTHYDTKVISLPAGVNEYYDSDTWAYDNARGSAFSPADILA"
     assembly_gap    3905..3928
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3929..4502)
                     /locus_tag="PK198_11895"
     CDS             complement(<3929..4502)
                     /locus_tag="PK198_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39479.1"
                     /translation="MIAHISLTVKALKRLALVVVLLGAGMISAYAQQKVTGVVKDDSG
                     APLAGVTVMVRGTTTGTVTDIDGTYSITANQGQDLEFSMVGMTAQRVLVGRQTTINVT
                     MGETAYNLGEAVVTALGIKEDRRKLSYSHQSVKGEELASTQRDNAFLSLQGRIAGLNL
                     TPTSGLAGGSVNINLRGVNSIGSSNQPLIVM"
     gene            4635..>6065
                     /locus_tag="PK198_11900"
     CDS             4635..>6065
                     /locus_tag="PK198_11900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF39480.1"
                     /translation="MNTRLRSVLLLLSACFCIGSASAQAPKRMNSAEIYESIQKLKFL
                     GSALYVAAHPDDENTRMISYLANEVKAHTAYLSLTRGDGGQNLIGPEISELLGIIRTQ
                     ELLAARRLDGGHQLFSRANDFGFSKDPDETFNIWGRQAILADVVWAIRQWQPDIIINR
                     FDHRTPGTTHGHHTASAVLAFDAFDLTANPKAFPEQLKHTSVWQPHRLFYNTSWFFFG
                     SREAFDKADKSHLYAMDLGVYYPLKGKSNPEIAAESRSMHRCQGFGSMGSRGSEMEYL
                     ELLKGDKPAVSNDPFAGINTTWSRVPAGESVAKILTEVEREFRFDNPAASLPKLMQAY
                     EHIQKLPDGYWKRVKLPEIETVIRACAGLFSEAVATDVSATPGQEVTLNIEVINRSAA
                     NIELRAIRLLPSGLDTAMQLALADNIKFTAQKKVRLPQNLSISNAYWLNESWEQGLFT
                     VQDQTLVGLPETPRQVKVQFEARIAGR"
     assembly_gap    6066..6081
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6082..7240
                     /locus_tag="PK198_11905"
     CDS             <6082..7240
                     /locus_tag="PK198_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HRF39481.1"
                     /translation="DVVFKKVDPDRGEVYQPFEITPPVFANMKDGVYVFAGSDPKTVE
                     LTVKAGMPELNGWVSWKLPKGWRIEPATQHFTMKNKSEEQTLRFQLFPPDGASEGRLT
                     PQIRVGDQTDGDETVYDKEIVTIQYEHIPTQTVLRTAGARIARLDLRKSGQQVGYIMG
                     AGDEVPAALRQIGYDVSELKDADITPENLRRFDAVILGVRAYNTLDRIKFHQLRLLEY
                     VKGGGNLIVQYNTNNGLLLPQNEIAPFPMKLSRERVTVEEAEMRILQPQHPVLNTPNK
                     IGAKDFEGWVQERGLYFPNEWDAAFSAIFSCNDPGQKPADGSLLIAKYGEGHYIYTGL
                     AFFRQLPAGVTGAYRLMANMISLGRSEKPAPVVPELPAPVVPAKGKKNKKG"
     gene            7244..8335
                     /locus_tag="PK198_11910"
     CDS             7244..8335
                     /locus_tag="PK198_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF39482.1"
                     /translation="MLKKLDIYILGKFLSTFFFTVLIFTLISVFIDFSEKVEKFITEP
                     ITRREILLIYYPGFIVFIAKILWPLYTLIAVIFFTSRMASNSEILSVFNAGVSFRRLM
                     RPYLVGAGVLMVIFLTGSHFFVPWISKLRLEVVHTHLDKNSDKGKTGNVHIFTAPGEK
                     VFVQQFRKADSTARNFRLERFDSSGLVFLLQADQAQYQGGPPHRWKVKNFIIRTFNGE
                     EEGIFMGRGMEADTTINLHPGDFIDYKEQQDMMTTPDLLRYIRQQKERGAGNTRKYES
                     ELHTRTAEAVTILILTVIGMAVAARKVRGGIGLHLALGISIGGLYVMLSRFAIVFATG
                     EAMPVLLGIWMPNLIFGAVALYLVSKAQK"
     gene            8399..9295
                     /locus_tag="PK198_11915"
     CDS             8399..9295
                     /locus_tag="PK198_11915"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRF39483.1"
                     /translation="MLSIDPKSIKPVDLHQYLLACVAPRPIAFVSTMDAEGRPNLAPY
                     SFFNAFSSNPPVLVFSSNRKASDNTTKDTLHNVRETGELVVNAVSFSMARQMALASVE
                     YPPEINEFEKAGFTPLASSLVRPYRVAESPAQLECRVREIITLGDQGGAGHLIICDVV
                     MLHINENVLDERGRVDPHKIDLVGRMGRFYYARASGEAVYNIEQRVDRFGMGFDQLPA
                     GIRHSRVLTGNHLAQLAALPQPPDADAIAAIGEDPRMAAIRNAAHPLDELHRLVQMEL
                     AKEDVATAACMAWWGEVALGNQ"
     gene            complement(9289..>10051)
                     /locus_tag="PK198_11920"
     CDS             complement(9289..>10051)
                     /locus_tag="PK198_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HRF39484.1"
                     /translation="GQRKGARYFFDAPVEEIRVQNGQANGIRVGGEWRDYDLVISNMD
                     AWFTYRKLLPGQPAPERSLHQEKSTSALIFYWGIRRSFPELDLHNIFFSNDYRREFEH
                     LAVGKISEDPTVYINITSKYTPADAPEGCENWFTMVNVPYNSGQDWDALIAETRQNVL
                     AKLSRILGTEVEPLIACESLLDPRSIESKTSSHLGALYGTSSNTRMAAFMRHPNFSHR
                     IQHLYFCGGSVHPGGGIPLALLSGKIVAEMIASDY"
BASE COUNT         2140 a         2728 c         2858 g         2271 t
ORIGIN      
        1 ccacagagag acatactttt gcagcatgat ctggctgagc gtgagggcat tggcatcctg
       61 cggcacggct gcgctggcca tgtaagcatc gcggttggcg gccgttactc cagcgtagtc
      121 catgtgagca atgatccctt ttctgaaagc atcgtaggcc agtgttttgt tgcctttttt
      181 gaaggcagcc tcggctttga tgaactgaat ttcagagtag gtcatcagcg agtgcttggc
      241 cttgtcggta aagatgtact tgccgggaat ttgggccgcc acggttacgg taggcgatac
      301 gtcccagagc agcggaatgc gagccatgtt gccggtggtg ttggtcgggt cgccggcagt
      361 aggcgttacg ccgcgatata cgccgtcggg agatgcgctg agcattttgg gcagacgcgg
      421 gtcttgcacg ccactgaaca ccgtaccatc gagcaggctc agaatgaagg cactctggcg
      481 gtaagagcgg aggttgttgc gggtaggccc gtaaaagttg ccgtcggcgc tgctggtacc
      541 tgcatgcggc acgtcaaagt tatcggcatt ggaagccaat gccttgtcgc aatactcaat
      601 caccttgtcg ggattgtagg aagccttgtt ggagacgtga ttggccaaac gagccaatac
      661 gccgtaggtg aacttcgtcc acttgctgcg gtcgccggca taaacgaggt cgccgcgggc
      721 gaggcttgcc tgactcacgc cgccgtcggt tttggccaga tcggcgacgg catctaatgc
      781 aatccgctct gcttctgcgt aaattttgtc ctgcgtatcg tagtcgaaca catagcggtt
      841 gggttcaaac atctgcgtga ggatcatttc gccatacacg tcggtggagg tttgccagct
      901 ccaggcgcgg atggatttgc ccacgcccac atagtcccat tgagatttga gcgtagcctg
      961 ctcaatcatc agctctatgt ttttgccgat ggcccaatag tgctggcgcc atttttcgcc
     1021 ggcggcatcg gagccgggcg cgtagccgtg ctgatcccac acgttattgg cggcaatcca
     1081 gttccagttt tggatgtact ggcccacata gcgggtatcg aatgtttcac cgcgcgtcat
     1141 attggccagc atgggcggaa gcagtgcgaa gccttcggcc acctgcgggt tgctggggtc
     1201 gaagttgatg tctaaccagt cgttgcaact gttggaaaac accaacatga aagcaaggat
     1261 ggccggcaga attattttac gtttcataat tatcatgttt ttgaatgatt agaaagaagc
     1321 cgacaaaccg atggagaagg tacgtggcag agaaatcttg ccgaagtcca taccgaaacc
     1381 gcctgcgccg ccggtagcgg ggttggtggt gctcacatac gggtctgcgc cggtgtagtt
     1441 ggtgatgagg agcaggtcgg tgccgttcac aaacacactc aactctttga tcacctgatt
     1501 cgggcctatc cactttccgg tcaggttgta gccgatggtt acgtcgcgga ggcgcagcca
     1561 gttgatgtcg cgctctacga agtcttcggg caacaaagct gttgtgtaga acaactcatt
     1621 ggcgctgggc gtgatgagct tgttgttggc cgtcggattg tcggtttcct cattgccgtc
     1681 gcggagtacg ctgttgccct ctgcaaagat gaaagcttcg tcgcggttga gggtgcgggt
     1741 gctcaagccg ttgcgtacga ggaacaactc gttgccgttg aagatgtcgc cgcctttgcg
     1801 aatgtcaagc aggaaggaga ccgtcagatt cttcataatg cggaaatcat tgaccaaacc
     1861 gatggtgaag tccgggttgc ggtcgccgat gggcaggaag ttggagttgg caatcgggaa
     1921 gcccgtcgtc gggttgatga gaatctgccc tttgctgttg cgcaggtagc tgaaaccacc
     1981 gatggccgta gccgatccgg cgccgcgatc atgcagaccg ggatagtagc ggttgccgnn
     2041 nnnnnnnnnn nnagcgagga tatcggcggg cgagaaagcg ctgccacgag cgttgtcgta
     2101 agcccaggtg tcggaatcgt agtattcgtt cacgccggcc ggcaggctga tgaccttggt
     2161 atcgtagtgc gtgaagttgg tgttgaggcg ccaggtgaaa ttgtcttttt tcaccggtgt
     2221 gaggccgagc tgaatttcga tgcccttggt gttgagttcg ccgccattga gcaggccgaa
     2281 aataaagccg gtgccgtagc tgaggcgctg cgtcacgatc tggtctttga tggttttgga
     2341 gaaccaagcc acatccaact gcacgcggcc tttgaagaag cccaggtcgg tgccgatctc
     2401 gaagctttcc accttttccg gtttcaggtt tgtgttgtcg ccgaagaagt cataagcaaa
     2461 acctccgccg gtagtcgttt gcggtacgag gcgggcacgg atgcgataag gcgttgccgg
     2521 gttgcctacc tgagcgtaag aggcgcggag cttaccaaaa tcgaggaagc cgagtttgcc
     2581 ttgcaagctg ggcatgtcgg agaatacata cgtcaaaccg gcagacggat agaagtagga
     2641 ctggttctca acgggcaacg tggaagacca gtcattccgg cctgttacgt tcacgatgag
     2701 ccaattctga tagttgattt cacctttggc aaacgcgccg gcgaggcggg tacgcgtcag
     2761 gatggtgcgg ttgcgctgtg tggtcgggtc ggtattgttg atactgttga aatccggcag
     2821 gtagaatttc tcgccataaa gcgaactggt ttcattgcgg cggtcattgg tggccatacc
     2881 cacgatgagc gagccgcgaa ggttgccctg cgttttttgc aaagtagtaa acatcgtcgt
     2941 cgtcaggaag cgcccgatat cggtgtagtt ttccgtccag ccggctcttg ggaaaccaat
     3001 gttggattcc gggtggaaaa agcggttggc atcggtggca tacacgtcgg cgccgaaacg
     3061 gccggtcact gtccaccagg gtgtgatggt agcatctaat gtaatgttac ctatggtgcg
     3121 gtcggtgcgc tccgagcgtg catttttata cacattaaaa taaggattcg cattgtctgt
     3181 agccggcgtt gcgttggcca gcgtcaatcg gcgagtgcct tctttggtga gataatcgct
     3241 catcaactga tcggaagggt aacggagtgc ggctgtcagg tagccttcgg cgccgccggg
     3301 aggcaatata ttttcgtttt tagaaaaact gaaacgcgta aatgccttga gccagggcaa
     3361 caatttggcc tccgaactaa gggccgcgtt gacctgatcg taagtattgg taggaattac
     3421 gccattggtt ttcaggtagc ctgccgacaa acgatacgtc agtttttcgg cgccgccctc
     3481 aaaagaaagg tcgtggcgca tgttactgcc gcgctggaag aaatcgccga tgttgtcata
     3541 aaaagtagtg ccttcagcat atttcgggcc gaagaaatcc ggatcgttca cttcgactgc
     3601 gccgttgcgg ccccggccat atacctgctg aatttcagga aacaggtaaa gctcggaggt
     3661 ggcgaaacgc gtcttgtacg acaggcggcc gatgccggct ttgcccttgt tggtcgtgat
     3721 gacgataacg ccgttggcgc cttcgttgcc gtacagggcg gcagcttccg ggcctttgag
     3781 caccgtcacg ctttcgatgt cgttcgggtt caactcggcc aaacggctgc ctgcttcgtc
     3841 gcggttgttg ttcacgttgc cgttgatgcc cacgccgtct gagtacaggt tgtgttggtt
     3901 gagcnnnnnn nnnnnnnnnn nnnnnnnncc ataacgatga gcggctggtt ggaactgccg
     3961 atggagttca ccccgcgcag gttgatgttg accgaaccgc cggccaaacc gctggtagga
     4021 gtcaggttca agcccgcaat acggccctgc aaagagagga aggcattgtc gcgttgggtg
     4081 ctggccagtt cttcaccttt gaccgactgg tgcgagtagc tcagcttgcg ccggtcctct
     4141 ttgataccga gggctgtaac gaccgcctcg ccaaggttgt aggcagtttc gcccatcgtt
     4201 acattgatgg tcgtttggcg gcccaccagc actcgttgtg cggtcatgcc caccatagag
     4261 aactctaggt cttgcccctg gttggcagta atgctgtaag tgccgtcaat gtcggtgacg
     4321 gtgccggtgg tggtgcctcg aaccataacg gtgacacctg ccagcggcgc acccgagtcg
     4381 tctttcacca caccagtgac tttctgttgt gcgtaggccg agatcatacc ggcccccaac
     4441 agcacaacaa ccaatgcaag gcgtttcaac gccttgacgg ttagggaaat gtgtgcaatc
     4501 ataatactac atttagttgt gaaataacct ctttcacacc aaagttagca ttgattttgc
     4561 aacaggcaat gttttgagaa ttttttttga cgggcagcgt atctttgccg tcttctttga
     4621 tacaacctta cgatatgaac acccgacttc gcagcgtgct gcttctgctt tcagcctgtt
     4681 tttgcatcgg ctctgcatcg gcgcaggcgc ccaaacgcat gaattccgcc gagatttatg
     4741 agtccatcca aaaactcaag tttctcggct cggctctgta cgtcgccgcg caccccgacg
     4801 acgaaaacac ccgcatgatc agctatttgg ccaatgaagt aaaggcccat actgcctatc
     4861 tttctctcac acgcggcgac ggcggccaga atctcatcgg gccggaaatc agcgagctgc
     4921 tgggcatcat tcgcacgcag gaactgctgg cggcccgacg gttggatggc ggtcatcagc
     4981 ttttcagccg cgccaatgac ttcggctttt ccaaagaccc cgacgaaacc ttcaacatct
     5041 ggggccgcca agccatactg gccgatgtgg tctgggccat tcgccaatgg cagcccgaca
     5101 tcatcatcaa ccgcttcgac caccgcacgc ccggcactac gcacggccac cacaccgcct
     5161 cggcagtgct cgccttcgat gcgtttgatc tcacggccaa tcccaaagcc ttccccgaac
     5221 aactcaagca cacctctgtc tggcagccgc accgcttgtt ttacaatacg tcctggttct
     5281 ttttcggctc ccgcgaggca tttgacaaag ccgacaaatc gcatctgtac gccatggatt
     5341 tgggcgttta ctacccactc aagggcaaat ccaaccccga aattgccgcc gaaagccgca
     5401 gtatgcaccg ctgtcagggc tttggttcta tggggtcgcg gggcagcgaa atggagtatc
     5461 tcgaactgct caaaggagac aaacccgctg tgagcaacga tccctttgcc ggcatcaata
     5521 ccacctggag ccgggtgccc gcaggagaat cggtggccaa aatactgacc gaggtggaac
     5581 gcgaattccg cttcgacaat cccgccgcct ccttgcccaa actcatgcag gcctacgagc
     5641 acatccaaaa attgcccgac ggctactgga agcgtgtaaa attacctgaa atagagactg
     5701 ttatccgcgc ctgtgcaggt cttttttcgg aagccgtagc caccgatgta tctgccacgc
     5761 ccggtcagga agtgacgctc aatatcgaag tcatcaaccg ctcggcggcc aatatagaac
     5821 tgcgggccat ccgtttgttg ccttcgggtt tggacactgc catgcagctt gcactggccg
     5881 acaacatcaa atttactgcc caaaagaaag tgcgcctgcc tcaaaacctc tccatttcca
     5941 atgcgtactg gctcaacgaa tcctgggagc aggggctgtt caccgtgcag gaccaaacat
     6001 tagtgggcct gcccgaaacg ccccggcagg tgaaggtgca gtttgaggcg cgcattgccg
     6061 gaaggnnnnn nnnnnnnnnn nggacgtggt gtttaaaaaa gtggaccccg accgcggcga
     6121 agtgtatcag ccatttgaaa tcacgccgcc ggtgtttgcc aacatgaaag atggtgtgta
     6181 tgtgtttgcc ggttccgacc ccaaaaccgt agaattgaca gtgaaagccg gtatgccgga
     6241 gctaaatggc tgggtgagct ggaagctgcc gaagggttgg cgcatcgaac cggccacaca
     6301 gcattttacc atgaaaaaca agtcggaaga acaaaccctc cgcttccagc tcttcccgcc
     6361 cgacggcgct tctgaaggcc gtttaactcc gcaaatacgc gtcggagatc agacggacgg
     6421 ggatgaaacg gtgtatgaca aagaaatcgt gacgatacaa tacgagcaca ttcccacgca
     6481 gaccgtactg cgcaccgccg gagcgcgcat tgcccgtttg gacttgcgca agtcgggcca
     6541 acaggtgggc tacatcatgg gcgccggaga tgaagtaccc gctgccctgc gccaaatcgg
     6601 ctacgatgtg agcgaactga aagacgccga catcacgccc gaaaatctgc gccgtttcga
     6661 tgccgtcatc ctcggcgtgc gcgcttacaa caccttggac cgcatcaaat ttcaccagct
     6721 ccgtctgttg gagtacgtca aaggcggcgg caatctgatt gtgcaataca ataccaacaa
     6781 cggcctgctc cttccgcaaa acgagatagc gcccttcccg atgaagcttt cgcgggaacg
     6841 cgtcacggta gaggaagccg aaatgcggat tttgcagccg cagcaccccg tgctcaatac
     6901 gcccaataag atcggcgcca aagattttga aggctgggtg caggagcgcg gcctgtattt
     6961 tccgaacgaa tgggacgcag ccttttcggc catattttcc tgcaacgacc ccggccaaaa
     7021 acctgccgac gggagccttt tgattgccaa atacggcgag gggcactaca tttatacagg
     7081 cttggcgttt ttcaggcagt tgccggcggg cgtcaccgga gcttaccggc tcatggccaa
     7141 tatgatttca ctggggcgtt cggaaaaacc cgcgcctgtg gtacctgaac tgccggcgcc
     7201 cgtcgttccg gcaaaaggca aaaaaaacaa gaaaggataa accatgctca agaagctgga
     7261 tatttacatt ttgggcaaat ttctcagcac ctttttcttt acggtgttga tcttcacgct
     7321 catttcggtc ttcatagatt tcagcgagaa ggtagagaag ttcatcaccg agccgatcac
     7381 ccggcgcgag atactgctca tttattaccc cggcttcatt gtttttattg ccaaaatcct
     7441 gtggccgctc tacacgctca tcgcggtgat ttttttcacc tcgcgcatgg cctccaattc
     7501 cgaaattctg tctgtgttta atgcgggtgt cagtttcagg cgtttgatgc ggccttatct
     7561 cgtgggcgcg ggcgtgctga tggtcatttt cctgaccggc tcgcacttct tcgtgccctg
     7621 gatcagcaag ttgcgtttgg aggtggtaca tacgcatttg gacaaaaaca gcgacaaggg
     7681 caaaaccggc aacgtacata ttttcactgc gccgggcgag aaggtcttcg tgcagcaatt
     7741 ccgcaaagcc gacagcaccg cgcgcaattt cagattggaa cgcttcgaca gcagcgggtt
     7801 ggtattcttg cttcaggccg atcaggcgca gtatcagggc gggccgccgc accgttggaa
     7861 ggtgaaaaac ttcatcatcc gcactttcaa cggcgaggag gagggcattt ttatggggcg
     7921 cggcatggag gccgatacca ccatcaacct ccaccccggc gattttattg actacaaaga
     7981 gcagcaggac atgatgacta cgcccgacct gttgcgctac atccgtcaac aaaaagagcg
     8041 cggcgcgggc aatacccgca agtacgaaag cgagctgcac acccgcactg ctgaggcggt
     8101 taccatcctc attctcacgg tgataggcat ggcggtagct gcccgaaagg tgcgcggcgg
     8161 cataggtctg cacttagcgc tgggcatttc catcggcggt ttgtatgtga tgctgtctcg
     8221 ttttgccatc gtttttgcca ccggagaggc catgcccgtg ctgctcggca tttggatgcc
     8281 caatctgatt tttggggcgg tggcgctgta tttggtgtcg aaggcgcaga agtagttttt
     8341 tatctttctt ttgtccatag gaaggcagac ctttgtggcg caataaaatc actttccgat
     8401 gctctctatt gatcccaaat ccatcaaacc ggttgatctg caccagtact tgctggcctg
     8461 cgttgcgcct cgccccatcg ctttcgtcag tactatggat gcagaagggc ggcccaatct
     8521 ggcgccgtac agctttttca atgccttcag ttccaatccg ccggtattgg tattttcctc
     8581 caaccgcaaa gcctccgaca ataccaccaa agacaccctc cacaatgtgc gcgaaacagg
     8641 cgaattggtc gtcaatgccg tgagtttttc catggcgcgc caaatggcgc tggccagtgt
     8701 ggagtatccg cccgaaatca acgagtttga aaaagccggc ttcacgccac tggcctcttc
     8761 cctcgtgcga ccgtaccgcg tggccgaatc gccggcccag ttggaatgcc gcgtgcgcga
     8821 aatcatcacg ctgggcgatc agggtggcgc gggccacctc atcatctgcg atgtggtgat
     8881 gctgcacatc aacgaaaatg tgctcgacga acgcggccgg gtggaccccc acaaaataga
     8941 cctcgtgggc cgcatgggcc gcttttacta cgctcgcgcc agcggggaag cggtttacaa
     9001 cattgagcag cgggtggacc gcttcggcat gggtttcgat cagttgcccg ccggtattcg
     9061 ccacagccgg gtgctcaccg gcaatcacct cgcacaattg gccgccctgc cgcagccgcc
     9121 cgacgccgac gccatcgccg ccatcggcga agacccgcgc atggccgcca tccgaaatgc
     9181 cgcgcatccc ttagacgaac tgcatcgctt ggtgcaaatg gaattggcca aagaggacgt
     9241 ggcaaccgcc gcctgcatgg cctggtgggg cgaggtggcg ctggggaatc agtaatccga
     9301 ggcaatcatc tccgccacaa tcttccccga caacaaagcc agcggaatgc cgccgccggg
     9361 atgcacgctg ccgccgcaga agtacaaatg ctgaatgcga tgcgaaaaat tggggtgccg
     9421 catgaatgcc gccatgcgtg tgttcgagct ggtgccgtac aacgcgccga ggtgcgaaga
     9481 ggttttgctt tcgatgctgc gcgggtcgag caggctttcg caggcaatga gcggttctac
     9541 ttccgtgccg aggatgcgac tgagtttggc caatacattt tggcgcgttt ccgcaatgag
     9601 ggcatcccaa tcctgaccgc tgttgtaagg cacattcacc atcgtaaacc agttttcgca
     9661 gccctccggc gcatctgccg gcgtgtattt ggaggtgatg ttgatgtaaa cggtgggatc
     9721 ctcactgatt tttccgacgg ccagatgctc gaattcccgg cggtagtcat tgctgaaaaa
     9781 gatgttgtgc aggtccaatt ccgggaacga acgccggatg ccccagtaaa aaatgagcgc
     9841 cgaggtggac ttctcctgat gcagcgaccg ctccggcgcg ggctgtccgg gcagcaattt
     9901 gcggtaagta aaccaagcat ccatgttgct gattaccaga tcgtaatcgc gccattcgcc
     9961 gcctacgcgt atgccattgg cctggccgtt ttgcacccgg atttcttcca ccggcgcatc
    10021 gaaaaaatag cgggcgcctt tgcgttgccc c
//
LOCUS       DAORLF010000133        10043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3597_length_10043_cov_2.269123, whole genome shotgun sequence.
ACCESSION   DAORLF010000133 DAORLF010000000
VERSION     DAORLF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3597_length_10043_cov_2.269123"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PK198_11925"
     CDS             <1..1086
                     /locus_tag="PK198_11925"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF39485.1"
                     /translation="MDSSLPLLKGKRVALVVNHTSMVGPTHLADTLRALGISIQAIFA
                     PEHGFRGAADAGEKITDGKDPSTGIPLLSLYGQKRKPSADDLRNVDWVIFDIQDVGLR
                     YYTYISTLHYVMESCAENNKPLMILDRPNPNGHFIDGPMREKGFESFVGMHPVPVVHG
                     MTVGEYGRMINGEGWLPGAAKCRLEVITCSGYDHNTLYQIPIKPSPNLPDMRSIYLYA
                     STCFFEGTVASEGRGTPTPFQVYGHPDSGTGDYYFTPASMPGAQSPKLLGKRCRGYNL
                     SATPIEELSALHRIDLSHLIRFYQAFPDKENFFLPSKFIDKLAGSSALREQITAGKTE
                     AEIRATWQEGLAQYRSMRAKYLLYKDF"
     gene            complement(1092..2513)
                     /locus_tag="PK198_11930"
     CDS             complement(1092..2513)
                     /locus_tag="PK198_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39486.1"
                     /translation="MENSNLIFILRTFSATENRKFKKWLLSPVHNQREDVVQLYDYLM
                     NGEHLQQDKYLSKERIFSKLFAGETFDDARIRQTTHFLLKCVEDFLTYEETQTDEVRR
                     RLDLARVYRQRQLDKLYQKAARQAEELQQAADYRNAYFLRNEYLLQREHYAFLSEKQK
                     QRAMPLNLTEMSEALDRSFVADKLRQACLMLAHQTVYKTEYDMRLLQEVLQYVEQKQW
                     LDEPAIGIYYYGYKSTTEREEPAHFEQLKALIVRFGHQFPTSEARDIYLMAINYCIAR
                     MNAGAEVFIREAFELYRRGFEQHILIENETISRWTFLNVIMIALTLSEFDWAESFIEQ
                     YQNFLEETHKDNFVHFSKALLLYEKRDYGPAMLLLNTADYDDMLINLRSKALLMKMFY
                     EQDMLDSLESLLEATRTYMTRKKVMAYHKAFFSNLVQLTRKLLRINPYDAEQKAKLRE
                     EILQANPLTTKERNWLLAQLDRK"
     gene            2728..5652
                     /locus_tag="PK198_11935"
     CDS             2728..5652
                     /locus_tag="PK198_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39487.1"
                     /translation="MKGKEAGKKGTTPGSIKLLILLLFVASFFRTEAQGWQVSFGGNG
                     EDQGQALIQAVDHGFVLVGFSESYGADNDLDVFVVRTDADGKLVWANVYDEGFIEHGY
                     DLLETADNGLIIVGDIYNSAQDPGNANVYLLRLDKRGRMLWSRQFGGSGFEQGLAICA
                     VPGGGYAIIGRTKDTSNGQDDILLVRVDENGNELWTKTYGGNGDDRGTAIVALEDGFA
                     FTGYATNIVGGIGNDIVLYRVDAEGAILWREVFGTSGNEEAHALIRTYEGNLALAGSI
                     NNYNDAFVAKYNLAGNLLWTRIVGYGGLGDEAFAIQELPDRSLVIAGRTETSASNTDI
                     LLAKLDANGNQIWLKALGDRDKLDTGEDFVATKAGGFAVIGYNALDGVFFNDLTLTVA
                     DGSGNIITNTISGKVFRDQGSCQLDPTDTPLEGWLVQATSANRTYFGVTDAQGNYSIL
                     TDLGTYQVSLVPRVAEYWQNCVPGGYLINLSNAYDTAQLNFPVFQAINCPFLEVDITA
                     PYLQYCSQVEYTVHYRNLGPGVGQSAAVEVTLGNTLNFVSSSIPGTVQGNKVTFQLGN
                     VASTSSGSFTFLTNVPCEGILVGSAALVSALITPNEPCTPPNPNWDGASIKVSAQCEA
                     DSIRFFIRNDGDGGMEDPGNYIIIEDQVLLIQEPFQLDAGMERQKTVPNTGATYRLVA
                     EQSAFHPGQSYPTVAVEGCTQTGAYTTGQVTQFPEDDQDSFIDIDVQEIVGSNVLPAD
                     LRGYPKGYGDSFLISPNTDITYILFFANTTGTDTIDRIVIRDTLSALLNVHSIVPGSS
                     SHPYRWEVYGNGILKITFDDVQLLPGGSAADSTRQGFVKFKVSQKPNNPEGAQITNRA
                     AVFFDNYAPWLTNATSHQIGTFPQFITVSTDEPEFVPGVKINVFPNPFVESAVFEVEG
                     MEVKEAELLMYDGNGRVVERKVFFGNRLEYHHKNRLMSGVYFYLLRSEGRLLNSGKIT
                     VR"
     assembly_gap    5709..5737
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5938..>6151)
                     /locus_tag="PK198_11940"
     CDS             complement(5938..>6151)
                     /locus_tag="PK198_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39488.1"
                     /translation="KSAENLELSDCIKATQKDQSGSFFLPLQNLRIPKQDTVRNNPPN
                     ENHHLFANRIIFYNHTTPCLTIIYTY"
     assembly_gap    6152..6227
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6228..6420
                     /locus_tag="PK198_11945"
     CDS             <6228..6420
                     /locus_tag="PK198_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39489.1"
                     /translation="AHQRIVKPMAKIISMEKTVFIVALLCAAKVRLNSHCFYDLHHPA
                     KGFLSDRPVSFIPVFADQS"
     gene            complement(6463..7194)
                     /gene="ric"
                     /locus_tag="PK198_11950"
     CDS             complement(6463..7194)
                     /gene="ric"
                     /locus_tag="PK198_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRF39490.1"
                     /translation="MEQLPITTQKTVGETVASDYRTAAVFKQFGIDFCCGGKRSIGQV
                     CEQKGIDPMLLQQALEQAQTAAGRSEMNFDDWKLGFLADYIENKHHVYVQERLPQLVE
                     YADKVARVHGEKFPPNLEVAALVNTLRDELMGHLLKEERILFPYIRAMEKALLEGQPK
                     PIPHFGTAAGPVNVMEAEHEVAGEIMHRIREITNDYALPEDACNTWRVLWSLLAEFEE
                     DLHQHVHLENNILFPKALRMEEEVR"
     gene            complement(7338..8120)
                     /locus_tag="PK198_11955"
     CDS             complement(7338..8120)
                     /locus_tag="PK198_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39491.1"
                     /translation="MLKIMKYTLSDLLRSRWGLIYLGFFLLLTLSLLWLSGDVSKVVI
                     SLMNVILILCPLIAVMFGVMYFYNSREFTELLLAQPIRRTDIFWGQYLGLALSLSLSL
                     LLGIGLPFLLYGLLASSEVLNFFVLVSTGVFLSFIFSGLAFYIALRNDNRIRGFGLAI
                     LVWLFFAVIYDGVFLLSLMLLKEYPLEHYSIVAAMFNPIDLSRILILLKLDISALMGY
                     TGAVFRKFLGTGLGSTLSFGVLVLWVLVPAWSIRRLALRKDF"
     assembly_gap    8158..8213
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8214..>8598)
                     /locus_tag="PK198_11960"
     CDS             complement(<8214..>8598)
                     /locus_tag="PK198_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39492.1"
                     /translation="GNLSGGTKQKVNIVLAFMYDCPTVILDEPTSGLDPVAMIHLREL
                     LNEAREQGKTILLSTHIMSFVEEMADEVVFLLEGKIRFRGSLQTLKEQYQEENVERAI
                     AHILQGKQAVEGNDGNSQTAGYKLKA"
     assembly_gap    8599..8633
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8634..8980)
                     /locus_tag="PK198_11965"
     CDS             complement(<8634..8980)
                     /locus_tag="PK198_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF39493.1"
                     /translation="MIEIRNLHKSYGKLEVLQGIDLGFEQKGRIAAILGPNGSGKTTL
                     IKSILGMVLPDKGEIRVDGQAIRQQWAYRERISYLPQIARFPENLSVRELIAMIKDLR
                     GGHAEDELLIRLFG"
     gene            complement(9082..>10043)
                     /gene="nosD"
                     /locus_tag="PK198_11970"
     CDS             complement(9082..>10043)
                     /gene="nosD"
                     /locus_tag="PK198_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRF39494.1"
                     /translation="NSGHNQLKDLAAIRVQRTKGYIIRSNILLNSYFGIYLEYAGAGE
                     VYGNTIIGQAVTEANAGNAIHAWYVQGVNISHNFLKGHRDGIYLEFVTHSFIHDNHST
                     GNVRYGLHYMFSNDDAYYCNTFDHNGAGVAVMFSKRIVMTDNYFEHNWGRASYGLLLK
                     EITDADIARNAFVSNTVGIMLEGASRIRYQNNQFKSNGWAVQVTGGCEDNNFIANNFL
                     SNALDLVINGRLNSNTFDGNYWSEYNGYDLDRDGRGDVPHRPVKLFSYIISQSPESII
                     LLRSFFVDLLNFSEKVSPALTPDNVLDNMPLMEEAQWAVGSRR"
BASE COUNT         2333 a         2626 c         2494 g         2394 t
ORIGIN      
        1 atggactctt ccctgccgct cctcaagggc aaaagagtgg cgctggtggt caatcatacc
       61 tctatggtag gcccaactca tctggctgat accctgcgcg cactcggcat ttccattcag
      121 gccatttttg cgcccgaaca cggttttcgc ggcgctgccg atgccggtga aaaaatcacc
      181 gacggcaaag acccttccac cggtattccg ctcctttcgc tctacggcca aaaacgcaaa
      241 ccctctgcgg acgacctccg caacgtggac tgggtcatct tcgatattca ggacgtgggg
      301 ctgcgctatt acacctacat ttctacgctg cattatgtga tggaatcctg cgcggagaac
      361 aacaaaccgc tcatgatcct ggaccggccc aatcccaacg gccatttcat agacggcccg
      421 atgcgggaaa agggcttcga gtcttttgtt ggcatgcacc ccgtgccggt cgttcatggc
      481 atgacggtgg gagaatacgg ccgcatgatc aacggcgaag gctggctgcc gggcgctgcc
      541 aaatgccgtc tggaggtcat cacctgctcc ggctatgatc acaacacgct ctatcagatt
      601 cccatcaaac cctcgcccaa cctgcccgat atgcgctcca tttacctcta tgcctccact
      661 tgtttttttg aaggcacggt ggccagcgaa ggccggggca cacccacgcc gtttcaggtg
      721 tatggccacc ccgacagcgg cacgggtgat tactacttta caccggcctc catgccgggc
      781 gcacaaagcc cgaaattgct gggcaaacgc tgccggggct acaatctctc tgccactccg
      841 atagaagaac tcagtgccct gcaccgcatt gacctctcgc acctcatccg cttttatcag
      901 gcattccccg ataaggaaaa cttcttcctg ccttcaaaat tcatagacaa attggccggc
      961 agcagcgccc tccgcgagca aatcacggca ggaaaaacgg aagccgaaat ccgcgctacc
     1021 tggcaggaag gattggcaca gtatcgcagc atgagagcca agtatttgct gtacaaggat
     1081 ttttagaagg tttacttcct gtccaattga gccagcagcc agttgcgttc tttggtggtc
     1141 aagggattgg cttgcagtat ttcctcgcgg agcttggctt tttgctcggc atcgtaagga
     1201 ttgatacgca gtagtttacg cgtgagttgc accagattgg agaaaaaggc tttgtggtaa
     1261 gccatcacct tttttcgggt catgtaggtg cgcgtagctt ccagaaggga ttccagcgag
     1321 tccagcatat cctgttcgta aaacatcttc atcaacagcg ctttagagcg cagattgatg
     1381 agcatgtcgt cgtagtcggc ggtgtttagc aggagcatgg ccgggccgta gtcgcgtttt
     1441 tcgtataaaa ggagcgcttt gctgaaatgc acgaagttgt ctttgtgggt ttcctccaaa
     1501 aagttctgat attgttctat gaacgattcc gcccagtcga actctgagag cgtgagggca
     1561 atcatgatca cgttgaggaa cgtccatcgg gagatggtct cgttttcgat gagaatgtgc
     1621 tgctcgaaac cccgcctgta cagctcaaat gcctcgcgaa taaagacctc cgcaccggcg
     1681 ttcatgcgcg ctatgcagta gttgatggcc atgagataaa tgtcgcgggc ttcggaagtg
     1741 ggaaattgat gcccgaatct gacgatgagc gctttgagtt gttcgaagtg cgccggttct
     1801 tcccgctcgg tggtggattt gtagccataa taataaatac cgatggccgg ctcgtccaac
     1861 cactgtttct gctctacata ttgcagcact tcctgaagca ggcgcatgtc gtactctgtt
     1921 ttgtaaaccg tttggtgggc cagcatcagg caggcttgcc gaagcttgtc ggccacaaaa
     1981 ctgcggtcca gcgcttccga catctccgtg agattgaggg gcatggcgcg ttgtttctgt
     2041 ttttcactca gaaaagcgta gtgttcgcgt tgcagcaggt actcgtttct cagaaagtag
     2101 gcattgcgat aatcggcggc ttgttgcagc tcctccgctt gccgggcggc tttttgatag
     2161 agcttatcca actgccgttg ccggtacact cttgccagat cgagccggcg gcgcacttcg
     2221 tcggtttggg tttcttcgta ggtcaggaag tcttccacgc acttgagcaa aaagtgcgtc
     2281 gtttgtcgga tacgggcatc atcgaaggtt tctccggcga agagcttgga aaaaatgcgt
     2341 tctttgctta ggtatttgtc ttgttgaagg tgttctccat tcatcagata atcgtacaac
     2401 tgcaccacat cttcgcgttg gttgtgtacc ggagacagca gccacttttt gaactttcgg
     2461 ttttcagtag cagaaaacgt cctgagaata aaaataagat tgctgttttc cataagattt
     2521 tggccttttt ttttcaacaa acgacctttt ttgattttta ttttaaatac ttgaaaacca
     2581 ataccaatga aaactattat gcacaatttt ttacgcaaaa aacatcattt ttttttaaat
     2641 ttagccagta atgtgttctt gtgtggcgcg aaaagtgact ccatctttgt atcggattca
     2701 gcaatgacca cgattaaaaa taaactcatg aaaggaaaag aggcaggcaa aaaaggcaca
     2761 acgccgggca gcataaagtt gctgatcctg ttgttgttcg tcgcctcctt cttccgcaca
     2821 gaggctcagg gctggcaggt ttccttcggc ggcaacggcg aagaccaggg ccaggcactg
     2881 attcaggctg tagatcacgg gtttgttttg gtcggattca gcgaatccta cggcgcagat
     2941 aacgatctcg acgtatttgt ggtacgaacc gatgcggacg gaaaactcgt ttgggccaac
     3001 gtttatgacg aaggcttcat cgaacacggt tacgacctcc ttgaaactgc ggataacgga
     3061 ttgatcatcg tcggagatat ttacaactct gcacaggatc caggtaacgc caatgtatat
     3121 cttcttcgtt tggataagcg gggcagaatg ctttggagtc gccagttcgg cggttccggc
     3181 ttcgaacaag ggcttgctat ttgcgctgtg cccggcggcg gctacgccat catcggtcgc
     3241 accaaagaca catccaacgg ccaggacgac atcctgctcg ttcgggtaga tgaaaacggc
     3301 aacgaacttt ggacgaagac ttacggcggc aacggcgacg acagaggcac ggccatcgtg
     3361 gcgctcgaag acggatttgc ctttaccggc tatgcaacca atatcgtagg cggcatcggc
     3421 aatgacatcg tactctaccg ggtggatgca gaaggcgcaa tcttgtggcg cgaagtgttc
     3481 ggcacctccg gtaacgaaga agcgcacgca cttattcgta cttacgaggg aaatctggcc
     3541 ttggcgggca gcatcaacaa ctacaacgat gcttttgtag ccaagtacaa tcttgccggc
     3601 aacctgctct ggacgcgtat cgtaggctac ggcggactag gcgatgaggc tttcgccatt
     3661 caggaattgc ccgaccggag cctcgtcatt gcaggtcgta cagaaaccag cgcttccaac
     3721 accgacatct tgctggctaa attggacgcc aacggcaacc aaatatggct caaagcttta
     3781 ggcgacaggg acaagctcga taccggtgaa gattttgtag ctaccaaggc cggcggtttt
     3841 gccgtcattg gctacaatgc gctggacgga gtgttcttca acgacctcac cctcacggta
     3901 gccgacggca gcggaaacat catcaccaat accatcagcg gtaaggtgtt ccgcgatcag
     3961 ggcagttgcc agttggaccc gaccgatacg ccgctcgaag gctggctcgt tcaggctact
     4021 tctgccaaca gaacctactt cggcgtgacg gacgcacaag gcaactacag catccttacc
     4081 gatctgggta cttatcaggt atctttagtg ccgcgtgttg ctgaatactg gcaaaactgc
     4141 gtgccgggag gctatctgat caacctgagc aacgcttacg atacggctca actcaacttt
     4201 cctgtctttc aggccattaa ttgccctttt ctggaagttg atattacagc gccctatctt
     4261 cagtactgct ctcaggtgga atacacggtt cactaccgca acctggggcc tggcgtagga
     4321 caaagcgcgg cagtggaggt tacattgggc aatacgctca actttgtctc ctccagcatt
     4381 cctggaacgg tgcagggcaa taaagtcacc ttccaattag gcaacgttgc ttctaccagt
     4441 tccggcagct ttaccttcct taccaatgtg ccttgcgaag gcattctggt gggatcggca
     4501 gccttggtga gtgcgctcat aacgcccaac gaaccttgta cgccgcccaa tcccaactgg
     4561 gacggcgcca gcatcaaggt gtcggcacag tgcgaagcag attctatccg tttctttatc
     4621 cgcaacgacg gcgacggcgg catggaagat cctggcaact acattatcat tgaagatcag
     4681 gtacttctca ttcaggagcc gttccaattg gatgccggca tggagcgcca gaaaacggtg
     4741 cccaacacag gagcaaccta ccgcttggta gccgaacagt cggctttcca cccaggacaa
     4801 agttatccga ccgtagcagt agaaggctgc acacaaacgg gagcatatac gacgggtcag
     4861 gtgacccagt ttcctgaaga cgatcaggat tcgttcattg acattgatgt acaagaaata
     4921 gttggttcca atgttttgcc ggccgatttg agaggctatc ccaaaggcta cggggattcg
     4981 ttcctgatct cgccaaatac cgatattaca tacatcctgt tctttgctaa tactacagga
     5041 accgacacca ttgatcgcat cgtcatccgc gacacgcttt ctgcactgct caatgttcac
     5101 agcatcgtgc cgggcagcag cagccacccc taccggtggg aggtttacgg aaatggaatt
     5161 ctcaaaatta catttgatga tgtgcaatta ttaccaggcg gcagcgctgc cgactcgacg
     5221 cgccaagggt ttgtaaaatt caaagtatcc caaaaaccga acaatcccga aggcgctcaa
     5281 atcaccaacc gcgctgccgt cttttttgac aactacgcgc catggctcac gaacgcgacg
     5341 agccaccaaa taggcacttt cccgcagttc atcaccgtat caaccgatga accggaattc
     5401 gtgccaggcg tgaaaataaa tgtgtttccc aatccgttcg tagaatcggc tgtttttgag
     5461 gtagaaggaa tggaagtgaa agaggccgaa ttgttgatgt acgatggaaa cggacgggta
     5521 gtggaacgca aagtgttctt tggaaaccga ttggagtacc atcataaaaa ccgactgatg
     5581 tccggggtat atttttacct gctgagatcg gaaggtcgct tactcaacag cggaaagatt
     5641 acggtacgat aatcataatc ccaactacta cggttccctg cctctgcgca gggaaccact
     5701 acacctctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag aaaaaaacgt ttgtttaaag
     5761 attaggtcgt cttatttttt tcatgagatt atgatttgtt attagcgaga ggccgtacca
     5821 tgcgtggtac ggcttttttt ttgccggtac cccactcgtt aacaaaacag tctcctttta
     5881 cggaggcgcc gaaaaaaggt tacagaagaa agtgattttt tttgagtgaa ttttttgtca
     5941 gtaagtgtaa ataattgtta gacagggtgt tgtgtggttg taaaaaatga tccggttggc
     6001 aaaaagatgg tggttttcgt tggggggatt atttcttaca gtgtcctgct taggtattcg
     6061 caggttttgt agaggtagaa aaaagcttcc ggattggtct ttttgagttg ctttaataca
     6121 atccgacaac tccaaatttt cggcactttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc ccatcaacgc
     6241 attgtaaagc cgatggccaa aatcatcagt atggaaaaaa cggttttcat cgttgcactt
     6301 ttatgcgctg ctaaggtgag actgaatagc cattgcttct atgatcttca tcacccggct
     6361 aagggatttt tatcagacag acctgttagc tttatccccg tatttgccga tcaaagctaa
     6421 cagctctcgc ccgcaaaccg cctaccccgc gccacagcgc ggctaccgaa cttcctcctc
     6481 catccgcaaa gccttcggaa acaggatatt attctccaaa tgcacatgct ggtgcaggtc
     6541 ttcctcaaac tccgccaaca gcgaccacag cacgcgccag gtattgcagg catcctccgg
     6601 cagcgcataa tcattcgtga tctcccggat gcggtgcatg atctctccgg ccacctcgtg
     6661 ctcggcttcc atcacgttca ccggaccggc ggcagtcccg aaatgcggta tcggcttggg
     6721 ttggccctcc agcaacgcct tttccatcgc ccggatgtat ggaaacagaa tgcgctcctc
     6781 tttgagcagg tgccccatca gttcgtcgcg gagggtgttc accaaggcgg ctacctccag
     6841 attgggcgga aacttttcgc catgcacgcg ggccactttg tcggcgtatt ccaccaattg
     6901 cggcaagcgc tcctgcacat acacatggtg cttgttttcg atgtagtcgg ccagaaagcc
     6961 taatttccag tcgtcgaaat tcatctcgct gcggccggcg gcagtttgcg cctgttccaa
     7021 agcctgttgc aacagcatcg ggtcaatgcc cttttgctcg cacacctgtc cgatactgcg
     7081 ctttccgcca cagcaaaaat ctatgccgaa ctgcttgaac accgcagccg tgcggtagtc
     7141 ggaggcaacg gtttctccta ccgttttttg cgtcgtaatt ggaagttgtt ccatgattta
     7201 ttacaatttt agaatgatca atttttttgt tccgggttca aggtttcagg tttcatccgc
     7261 cgaagccttg gcgcaggcgg gcttcgggtt caaggttgag gcttgcagtt cgtggccagc
     7321 agctaacagc ccgcccatca aaagtccttc ctcagcgcca gtcgccggat actccatgcc
     7381 ggaaccagca cccacagcac cagcacccca aacgacaaag tcgaacccag ccccgtgccc
     7441 aaaaacttgc gaaacaccgc ccctgtatag cccatcagcg ccgaaatgtc gagcttgagc
     7501 aggatgagaa tgcgcgacaa atcaatggga ttgaacatag ccgccacaat ggaataatgc
     7561 tccagcgggt attctttgag cagcatcagc gaaagcagaa aaacgccgtc gtaaatgacc
     7621 gcaaagaaca gccacaccag tatcgccaag ccaaaacccc gaatgcgatt gtcgttgcgc
     7681 aaagcaatgt aaaatgccaa tccgctgaaa atgaacgata aaaatactcc tgtggaaacg
     7741 agtacgaaga aattcaatac ctccgaggag gccagcagac cgtacaaaag aaaaggcagc
     7801 cctatgccca gcaataagct aagagacaaa gacaaggcca agcctaagta ctgcccccag
     7861 aaaatatcgg tgcgccgaat gggctgcgcc aacagcagtt cggtgaattc gcgcgagttg
     7921 taaaaataca tcaccccaaa catcacggcg atcagcgggc acaaaatgag gatcacgttc
     7981 atcagactga tgaccacttt cgatacgtcg ccgctgagcc acagcaaaga caaggtgagc
     8041 aagaggaaaa accccagata tatgagcccc caacggcttc gcagcaggtc ggagagcgtg
     8101 tatttcatta ttttcagcat ggtttattca gttgtgaggg gtgagtaaat gaggggtnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagctttt
     8221 agcttgtagc cggcagtctg agagttgccg tcgttcccct ccaccgcctg ttttccctgc
     8281 aaaatatgag caatagcgcg ttccacattc tcttcctggt actgttcttt cagcgtttgc
     8341 aacgaacccc ggaaacgtat tttcccctcc agcaaaaaca ccacctcgtc ggccatttct
     8401 tccacaaaac tcatgatgtg cgtagagagc agaatggttt tgccctgttc gcgggcctca
     8461 ttcagcagtt cgcgcaggtg aatcatggcc accggatcga ggccggaagt cggttcgtcc
     8521 aagatgacgg tcggacaatc gtacataaac gccagcacga tgttcacctt ctgtttggtg
     8581 ccgccggaga ggttgccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaaaca
     8641 gccggatcag tagctcgtct tcggcatggc cgccgcgcag gtctttgatc atggcgatga
     8701 gttcgcgcac gctcaggttt tccgggaaac gggctatctg cggcaggtag ctgatgcgct
     8761 cccggtacgc ccactgctgc cggatggcct gcccgtccac cctgatttcg cctttgtcgg
     8821 gcagcaccat gccgagtatg ctcttgatga gcgtggtctt gcccgaccca ttggggccga
     8881 ggatggcggc aatacggcct ttctgctcaa agccgaggtc tatgccctgc aatacttcga
     8941 gcttgccgta agatttgtga agatttcgta tttcgatcat ggtctcttca ttgtttaatg
     9001 ttcaaggttt tacggtttaa ggttccgggt tgtagccgcc cactgcctac tgcccacccc
     9061 gcgccacggc gcggccaccg cctaccgcct actgcccacc gcccactgag cctcctccat
     9121 cagcggcata ttatccaaca cattatccgg cgtgagtgcc gggctgactt tttcagaaaa
     9181 attgagcaga tcaacaaaaa aactgcggag caaaatgatg gactccggcg attgcgaaat
     9241 gatgtatgaa aatagcttca ccggccgatg cggcacatct ccgcgcccgt cgcggtcgag
     9301 gtcgtagcca ttgtattcgc tccagtagtt gccgtcaaag gtgttgctgt tcaggcggcc
     9361 attgatgacc aaatcgaggg cattgctgag aaagttgttg gcaatgaagt tgttatcttc
     9421 gcagccgccg gtcacctgca ccgcccagcc attcgatttg aactgattat tttggtaccg
     9481 gatgcgggaa gcgccttcga gcatgatgcc caccgtattg ctcacgaacg cattccgggc
     9541 aatgtctgca tcggttattt ccttcagcag cagcccgtaa gaagcgcgcc cccagttgtg
     9601 ttcaaagtaa ttgtccgtca tcacaatgcg cttcgaaaac ataaccgcca cgcccgctcc
     9661 gttgtggtca aacgtgttgc agtagtacgc gtcgtcgttc gagaacatgt agtgcagccc
     9721 atagcgcaca ttgcccgtgc tgtggttgtc gtgtatgaag ctgtgcgtca caaattccag
     9781 ataaatcccg tctctgtggc ctttcaaaaa attgtgactg atgttcactc cctgcacata
     9841 ccaggcatgt atggcgttgc ccgcattggc ctccgtcacg gcctgcccta tgatggtgtt
     9901 gccatacacc tcgcccgctc cggcgtattc gaggtaaatg ccgaagtagc tgttcagcag
     9961 gatgttgctt cgtatgatgt agcccttggt ccgttgcacc cgtatggcgg ctaagtcttt
    10021 gagttggttg tggccggagt tct
//
LOCUS       DAORLF010000134        10015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3618_length_10015_cov_2.083735, whole genome shotgun sequence.
ACCESSION   DAORLF010000134 DAORLF010000000
VERSION     DAORLF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_10015_cov_2.083735"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..574)
                     /locus_tag="PK198_11975"
     CDS             complement(56..574)
                     /locus_tag="PK198_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39495.1"
                     /translation="MKKLLFLSLLIAGAFAFYSCEKEVITPYVALTGTFTNTPDPSAG
                     FWQVPIPDGSTFMSPKKYIVDGTSPLFGEVDETKSFMELPSPVFEPKIGGFTGTCTIT
                     TVDKDGDQLIYVGEYYMFADFSNKTYLRIDGGTGKWEDAQGWFNTTGQVDPRTGVNTL
                     SGTGEVTQPQKK"
     gene            complement(767..>1780)
                     /locus_tag="PK198_11980"
     CDS             complement(767..>1780)
                     /locus_tag="PK198_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF39496.1"
                     /translation="QKHYEEGSGIGLTLVREYVKLMGGEITVQSKPGEGTEFTITLPV
                     SRQATPQDAAQSVQPASPVAEIHFDLLADEKMHVHEQTTTLPQLLLIEDNPELSQYLR
                     IILGPKFHLLTASNGEEGIRQAIEQVPDLILSDVMMPVKDGFELCRTLKKDFRTCHIP
                     IVLLTARADTASRITGLEQGADAYLTKPFNQRELFACLRNLFVQREKLRIKYAALPNV
                     SEPAPTPGLNEMFLSNVRSHLEKNYRNEAFNIENLYVALGISRVQLHRKLTALTGQST
                     SHFIRSFRLEKAKELLATTLKSVAEIAFETGFSDPNYFSRVFAQEYGMPPTDLRKGQE
                     RAA"
     assembly_gap    1781..1938
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1939..>2282)
                     /locus_tag="PK198_11985"
     CDS             complement(<1939..>2282)
                     /locus_tag="PK198_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF39497.1"
                     /translation="TLIDGMAELLQNDPQHQHLERTEAIQRNANKVLRLVDQMLNLAR
                     LEAGSMPLQPIQGDVTAFLRQVFQSFPGLAEYRKVRLHFLPDNQGFTMDFDPDKLEEL
                     VGNVLSNALKYT"
     assembly_gap    2283..2349
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2350..2982)
                     /locus_tag="PK198_11990"
     CDS             complement(<2350..2982)
                     /locus_tag="PK198_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39498.1"
                     /translation="MQHSKFLPMQVPFLRRLEGWIARLVSLPGDTEESLESKKAYLLA
                     LIAMTNMFLFLTLLGWWAGLPIIVQFGIGLLLFFIPQTILFIRLRKGLLWFVFISNTF
                     SLLLTTWTIIRLGGITHSCGLVFGGLTTVVYSMIYPNTRVLWSSFILYVLSLIVIALL
                     EPHLTPPPELTLEKNLLYFVLNAVWLSANILILIFYVYRKQADLATLKAQR"
     assembly_gap    3253..3380
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3381..4193)
                     /gene="hypE"
                     /locus_tag="PK198_11995"
     CDS             complement(<3381..4193)
                     /gene="hypE"
                     /locus_tag="PK198_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRF39499.1"
                     /translation="MSRHTSILSASCPMPKMDFDIITLGHGSGGLLTNKLLEAGVFDI
                     LKNELLDQKHDGVVLELNGKTAFSTDSFVVSPIFFPGGNIGELAINGTVNDIAMCGAR
                     AEYLSLAFVIEEGLPMTDFWDILLSIRQAADGAGVRIVTGDTKVVERGKGDQIFINTS
                     GVGRVHPRADISMKRVQAGDRIIVSGPIAAHGMAIMSVREGLEFESAIVSDTCHFNHI
                     VQQLLDEYGESVHLLRDATRGGVATGLNEVARDTGLGINIEEKRLAVSEEVGA"
     gene            complement(4203..>4713)
                     /gene="hypD"
                     /locus_tag="PK198_12000"
     CDS             complement(4203..>4713)
                     /gene="hypD"
                     /locus_tag="PK198_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRF39500.1"
                     /translation="MGIGEYYPLVERYHIPIVVTGFEPVDLLQGILMTVKQLEQGEAK
                     LENQYARVVRPEGNPDAVEVIEQVFETGDREWRGIGNIPRSGYQIRAAFAEYDAHLKF
                     SIDIEKAPENPDCIAGLVLKGIKKPHECPQFGKGCTPQMPLGAPMVSSEGACAAYYHF
                     SNAIEMQPS"
     assembly_gap    4714..4725
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4726..5309)
                     /gene="hypD"
                     /locus_tag="PK198_12005"
     CDS             complement(<4726..5309)
                     /gene="hypD"
                     /locus_tag="PK198_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRF39501.1"
                     /translation="MKFMSEYRDPALVEKYLNEIHKTVTRPWNIMEVCGGQTHSLVKN
                     GILRLLPEQVNMIHGAGCPVCVTPLHLIDKAVYLALEKGVMLCSYGDMLRVPGSQRSL
                     LEAKAQGADVRILYSPLEAVKLAQDHPDKEVVFFAVGFETTAPANALSVIHAQRLGIK
                     NYSILASHVLVPPAIEAVMEDEAAVIHGFLAAGHV"
     gene            5499..6242
                     /locus_tag="PK198_12010"
     CDS             5499..6242
                     /locus_tag="PK198_12010"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF39502.1"
                     /translation="MSSTSTDSLAAPVQGFQFQQFFIAQDRCAMKVGTDGVLLGAWVE
                     TEAAQSILDIGTGTGVIAVMLAQRNEHALVHGVEIDAEAAAQAAENILATLWADRLEA
                     RHYAIQDYARDTEQRYDLIVSNPPFFTGGVLSQSENKTGVRHTVKLPHGDLLRATQTL
                     LAPQGVFAVVLPLLEGLRFVEMARSYRLYCERMTEVRTKAGKPISRLLLQFTRHIPDA
                     VRKDELIIQEEETNNWTPAYRALTSGFYL"
     gene            6303..7319
                     /locus_tag="PK198_12015"
     CDS             6303..7319
                     /locus_tag="PK198_12015"
                     /inference="COORDINATES: protein motif:HMM:NF021059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HRF39503.1"
                     /translation="MDLNLLHRMIEEGYINVQKHPHYELFIYNYSAKAQYDYVWNEAT
                     KTCRGLIMDAEMRVAARPFAKFFNLEEADPTEIPNLPFDVFEKMDGSLGILYQVAGEC
                     FIATRGSFSSDQAHKANEILRSKYAAALPHIDPAKTYLFEIIYPGNRIVVDYGDREAL
                     VLIAVMDTATGQDLPLEDIGFPVVRQYHGIKDWRILKAMETDNHEGFVIRFQNGQRYK
                     VKFEEYLRLHRIVTQISSVNIWEHLAAGIPLDEYLERVPDEFYHWVKATSAELHNRFE
                     DIENQCKAAFKIFPTRKESAEYFARCAYPNVLFKMLDGRPYHDIIWKMVRPAFQKAFT
                     ASKE"
     gene            7343..8218
                     /locus_tag="PK198_12020"
     CDS             7343..8218
                     /locus_tag="PK198_12020"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39504.1"
                     /translation="MKKVIILRGLPGSGKSTFARQLLAETPGAYKRINRDDLRAMFDN
                     GHWSKGNEKFVKQVRDLLIVSALSEGKHVIVDDTNLAPHNETRIREVVREYNRTHNDS
                     VQVELKEMDTPLEECIAHDALRPQPVGAARIREMHRQFMAAAQASSMQQDESLPRAII
                     CDLDGTLALLNGRNPFDAAQCEKDVLNRPVAEVLKRMRDVGYKILLLSGRQSEHRQPT
                     LNWLARHEIPFDMLEMRATGDGRMDAVIKKELFDQHIRGRFYIEFVLDDRNQVVDLWR
                     RELGLSCFQVNYGDF"
     gene            complement(8269..>9658)
                     /locus_tag="PK198_12025"
     CDS             complement(8269..>9658)
                     /locus_tag="PK198_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39505.1"
                     /translation="LFGARNFTFGGEMAQAADGTVLLVGETMSYSDRTSFDGLILKTE
                     PNGNTIWLNAIDLGDQGRNTFVGVAPLSNGGCFVVGNYLPAGAEYILPNGHAYLARLD
                     TAGQLLWHRKIRPAGEQYGSWMSGIVPTTDGNFVMFGYTANADNRGSDQFYIKINDVG
                     EVIWQQYEEITGSAFKPYKAVALSGGELVFTGTNESTDINSQIYFQKIASDGNILWSV
                     EYDTYTLDFALAPTADGGIMFAGATVIPGIFSSIPFFIKLDENGDFQWIKTHVIGSFN
                     YATPYSIKPAPGGGYFVAGEILADTTDHFYDFMLLKIDENAEPLWVRNFGAKNTEWAE
                     DLLVAAPDQILLWGYNQLRPPVWNLHAVLARTDGEGNLSVGTKEPAPGENHRVVVFPN
                     PAGINTNIVLSPRPAKPVHWLLTDVNGRSVAQGITQTGILEMGLERYSAGMYYIVFPG
                     SSYPPARLVVAK"
     assembly_gap    9659..9706
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9707..>10015)
                     /locus_tag="PK198_12030"
     CDS             complement(<9707..>10015)
                     /locus_tag="PK198_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39506.1"
                     /translation="HLTFSPTLDVTSLRPIASNRPAVITRADNGLVHVVMDSLHLVPD
                     SAAEADAMFRFSIQPYADTPPEAGAGSIFKVDAKAYLDDAGPFPVASGFLNYSQSFPV
                     "
BASE COUNT         2204 a         2506 c         2656 g         2236 t
ORIGIN      
        1 cttcaaaata ctgccccgct gggggcaggg cagtacgaat tttgattgtg tctaactatt
       61 tcttttgggg ttgggtcact tcgcccgtac cgcttagtgt gttcactccg gtacgggggt
      121 caacttggcc agtcgtgttg aaccagcctt gtgcatcttc ccatttgcct gtgcctccgt
      181 caattcgcag gtaggttttg ttggaaaaat cggcgaacat gtagtactca cccacataaa
      241 tcaactggtc gccatccttg tccaccgtgg taatggtgca tgttccagtg aacccgccaa
      301 tttttggctc aaatacaggg cttggcagtt ccatgaagct cttggtttca tctacctccc
      361 cgaacagagg cgaagtgcca tctactatat attttttagg ggacataaaa gtggaaccat
      421 cagggatcgg tacttgccaa aaacctgcac ttgggtcggg ggtgttggtg aatgtgccgg
      481 tgagggcaac atagggcgtg atgacctctt tttcgcagga ataaaaagcg aaggcgcctg
      541 cgataagcag cgatagaaaa aggagctttt tcatggtgtt gaaatttaaa gtgaatgatc
      601 gagcatgtcc gccgaagact tcattcagaa ttttctcgtc ggcatatact cttttgacac
      661 ttcaaatatc agtatgtgag caagcaaaat tgcttactgc ctgtttcatc ttctggacag
      721 ccgggtacaa catctggatg cttgtttcaa aatctggagc aatgtttcag gctgcgcgtt
      781 cctggccttt tcgcaggtcg gtaggcggca ttccgtactc ctgcgcgaag acccggctga
      841 aatagttggg gtcgctgaat cccgtttcga aggcaatttc agcaacggat ttgagggtgg
      901 tggcgagcaa ttccttcgcc ttttcgagcc ggaagctgcg gatgaaatga gaagtggact
      961 gcccggtgag cgccgtcagt ttgcggtgca gttgcacccg gctgatgccg agggcaacgt
     1021 aaaggttttc gatattgaat gcctcgttgc ggtagttttt ctccaagtgg ctgcgcacat
     1081 tcgacaaaaa catttcgttc aggccgggcg tgggggcagg ctcgcttaca ttgggcaggg
     1141 cagcgtattt gatgcgcagt ttttcccgct gcacgaagag attgcgcagg caggcgaaga
     1201 gttcccgctg attgaaaggc ttggtcaggt aggcatcggc gccctgttcc agcccggtga
     1261 tgcgactggc ggtatcggcc ctggcggtga gcaggacgat gggaatatgg caggtacgga
     1321 agtctttttt caatgtgcgg cacagctcga agccgtcctt caccggcatc atcacatcgc
     1381 tgaggatgag gtcgggcacc tgctcgatgg cctgccggat accttcttcc ccattggagg
     1441 cggtgagtaa atggaatttc ggaccgagaa tgatgcgcag gtattggctg agttcggggt
     1501 tgtcttcgat gaggaggagt tggggcaggg ttgtagtttg ttcgtgaaca tgcatcttct
     1561 catccgcaag gaggtcgaag tggatttcgg cgacgggcga tgccggctgg acggactgag
     1621 ccgcatcctg cggggttgcc tgccggctga cgggtagcgt tatggtgaac tccgtgcctt
     1681 cgccgggttt gctctgtacg gtgatttcac cgcccatcag tttcacatat tcccgcacga
     1741 gggtgaggcc gatgccgctg ccttcttcgt agtgtttttg nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnngg gtgtatttca atgcattgga gagcacatta ccgaccaatt
     1981 cctccaattt gtcggggtca aaatccattg tgaacccttg gttatcaggc agaaaatgca
     2041 ggcgtacctt gcggtattcc gccaggccgg ggaaagactg gaacacctgc cgcaggaagg
     2101 ccgtcacgtc tccttgtatg ggttgcagag gcatggagcc ggcctccaaa cgggcgaggt
     2161 tgagcatttg gtccacaagg cggagtactt tgttggcgtt gcgctgaatg gcctctgtgc
     2221 gctccagatg ctggtgctgc gggtcgtttt ggagcagttc ggccataccg tcgatgagcg
     2281 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnc cgctgggctt tcagggtagc caaatctgcc tgctttcgat agacgtaaaa
     2401 aatcagaatg agaatattgg ccgatagcca aacggcattc aggacgaagt acagcaagtt
     2461 tttctctaag gtcagctccg gcggaggtgt cagatggggt tccagcaggg caatgacgat
     2521 cagggagaga acatacagga tgaagctcga ccataaaact cttgtattgg gataaatcat
     2581 ggagtaaacc accgtggtga ggccaccgaa taccaatcca caggagtggg taatgccgcc
     2641 caggcggatg atagtccatg tagttaacag gaggctgaaa gtattgctga taaaaacgaa
     2701 ccacagaagc ccttttctca gccggatgaa aagaatggtt tggggaataa aaaagagcaa
     2761 cagccctatg ccgaactgta caatgatggg gaggccggcc caccaaccca gcaaggtgag
     2821 gaataagaac atgttggtca tggcgatcag ggccaggagg taagctttct tggactccag
     2881 cgattcttct gtatcgccgg gcagggagac gaggcgggct atccatccct ccaagcgtcg
     2941 taaaaagggg acttgcatgg gcaggaattt actgtgttgc aagactcaaa tgctccgttt
     3001 tcaaagccga agttaggtaa ttatttgata aaacagaggc atgattttac gaaacgccgc
     3061 ttgccccgga attctttgtg tatgtacaaa agggcggttc atttctttga atccaaagtg
     3121 cggtttcatc agcaaattct cggcaattgt tcgcccggca gcatgttgac gacccgccgc
     3181 ccgccgatgc tgctttgaag aatcacctgt ttgggatgtt gtcccgtcac ctcgcccagc
     3241 atgaaggctt gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn ggcgcctact tcttccgaca ctgcgagccg tttttcctct
     3421 atgttgattc ccaggccggt atcgcgggcc acctcgttga gtcccgtagc cacgccgccc
     3481 cgggtagcgt cgcgcagcag gtgaaccgac tcgccgtatt cgtcgagcag ttgttgtacg
     3541 atgtggttga aatggcaggt atcgctgacg atggcagact caaactccag cccttcgcgc
     3601 acgctcataa tggccatgcc gtgcgctgca atggggccgc tgacgatgat gcggtcgccg
     3661 gcctgcaccc gcttcatgga gatgtcggcg cgcggatgca cccgccctac gccggaggta
     3721 ttgatgaaaa tctgatcgcc cttgccccgt tccaccacct tggtatcgcc ggtgacgatg
     3781 cgaacgcccg ccccatctgc tgcctgccga atgctgagca ggatgtccca aaaatcggtc
     3841 atgggcagcc cttcttcaat gacgaaagcc aaagacaagt actcggcccg tgccccgcac
     3901 atggctatgt cgttgaccgt gccgttgatg gccaattcgc cgatgttgcc gccggggaaa
     3961 aatatgggcg acaccacgaa gctgtcggtg ctgaaggcag tttttccgtt cagttccagc
     4021 accacgccat cgtgtttttg gtctaacagc tcatttttga gaatgtcgaa aacgccggct
     4081 tccaggagct tgttggtgag caggccgccg ctgccgtggc cgagggtgat gatgtcgaaa
     4141 tccattttcg gcatcgggca ggaggcagat aaaatgctgg tgtgtcggga catggcgttg
     4201 ggttatgatg gttgcatttc tatagcattc gaaaaatggt agtatgccgc acatgcgccc
     4261 tcgctgctca ccatgggcgc gccaaggggc atttgcgggg tgcagccttt gccgaactgc
     4321 gggcactcgt gcggtttttt gatgcctttg agcaccaaac cggcaataca atccgggttt
     4381 tcgggcgcct tttctatgtc aatgctgaac ttgaggtggg cgtcgtattc cgcgaaagcg
     4441 gccctgatct gatagccgct gcggggtatg ttgccgatgc cgcgccactc gcggtcgccg
     4501 gtttcaaaaa cctgctcaat gacttccact gcatcggggt tgccttcggg gcgcaccacg
     4561 cgggcgtatt ggttttccaa cttggcctcg ccctgttcca actgcttgac agtcattaaa
     4621 ataccttgca acaaatctac cggctcgaac cccgtgacga cgatgggaat gtggtagcgc
     4681 tccaccaaag ggtagtactc gccgatgccc atgnnnnnnn nnnnnacatg cccggcggcc
     4741 agaaacccgt gaatgacggc ggcttcgtct tccatcactg cctcaatggc gggcggcacc
     4801 aaaacgtgcg aagccagtat ggagtaattt ttgatgccca aacgctgggc gtgtatgact
     4861 gagagcgcgt tggccggagc ggtagtttca aaacccacgg caaagaacac gacctctttg
     4921 tcggggtggt cctgggccaa tttgacggcc tccaatggcg agtacaaaat gcgcacatcg
     4981 gcgccttgtg ccttggcttc cagcaaacta cgctgagaac cgggcacgcg cagcatgtcg
     5041 ccgtaagagc aaagcatgac gcctttttcg agggccagat acaccgcttt gtctatgaga
     5101 tgcagcggcg tgacgcatac cgggcagccg gcgccgtgaa tcatgttgac ctgttcgggc
     5161 agcaggcgca ggatgccgtt tttgaccaag ctgtgggttt ggccgccgca tacctccatg
     5221 atgttccagg ggcgcgtgac ggttttgtgt atttcgttga gatacttttc taccaaggcc
     5281 ggatcgcggt attcgctcat gaatttcata ctcgcttgtt tttagtgagg atgccagtgt
     5341 caaacttgtt gcgaaagtag gggttcagag ttgaaaagca aagtgatttt tgtcaatcgg
     5401 agtttttgta ggtttctgaa gagtaagcaa tgcagcctct ttttgtcatc aaccaaacca
     5461 tccatgtagc ggttaataat ggcttttaac gatcaaacat gagcagcact tcaaccgatt
     5521 ccctcgccgc gcccgttcag ggttttcagt tccaacagtt ttttattgcc caagaccgct
     5581 gcgccatgaa ggtgggcacc gacggcgtgc tgctgggcgc ctgggtagaa actgaggccg
     5641 cccaaagcat cctggatatt ggaaccggca cgggcgtcat tgccgtcatg ctggcccaac
     5701 gcaacgaaca cgcgctggtt cacggagtgg aaattgacgc cgaggccgct gctcaagctg
     5761 ccgaaaacat actggctacc ctctgggccg accgcctcga agcacgccac tacgccatac
     5821 aagactacgc ccgcgacact gaacaacgct acgacctcat tgtcagcaat ccgccttttt
     5881 ttaccggcgg ggtgctgtcg caaagcgaaa acaaaaccgg cgttcggcac accgtcaaac
     5941 tgccgcacgg cgatctgctc agagctacgc agaccttgct cgctccgcag ggcgtgtttg
     6001 cagtggtgct gcccctgctc gaagggttgc gttttgtaga aatggcccgc agttaccgcc
     6061 tctactgcga gcgcatgacc gaggtgcgca ccaaagccgg caagcctatc agccgtttgt
     6121 tgctgcaatt cacccgccac atccccgatg ccgtccgaaa agacgaactc atcattcagg
     6181 aagaggagac caataactgg acgccggcgt accgggcgct cacgagtgga ttttatctgt
     6241 aaagaatttt ttcccgtcct tcgtggttgt tccgccattc tataaccagc ccttttgctg
     6301 aaatggactt gaacttattg caccggatga tcgaagaagg gtatatcaac gtacagaaac
     6361 accctcatta cgagttgttt atctacaact actcggccaa agcccagtac gactatgttt
     6421 ggaacgaggc aaccaaaacc tgccggggcc tcatcatgga tgcagaaatg cgcgtggctg
     6481 cgcggccatt tgccaagttt ttcaacctcg aagaagccga tccgaccgag attcccaacc
     6541 tgcccttcga tgtgtttgaa aaaatggacg gatcattggg tattttgtac caagtggccg
     6601 gcgagtgctt tatcgctacc cgtggttcgt tttccagcga tcaggcacac aaggccaacg
     6661 aaatattgcg ctcgaaatat gccgctgcac tgcctcatat tgacccggcc aaaacctatc
     6721 tgttcgagat catctatccc ggcaaccgca tcgtggtgga ctacggcgac cgcgaagcat
     6781 tggtactcat cgccgtcatg gataccgcta ccggccaaga cctgcctctg gaagacatcg
     6841 ggtttccggt ggtgcggcag tatcacggca tcaaagactg gcgcatcctc aaggcgatgg
     6901 aaaccgacaa ccacgagggc tttgtcattc gttttcaaaa cgggcagcgg tacaaagtga
     6961 aatttgagga atacctgcgc ctgcatcgca tcgtgacgca gatttccagc gtgaacattt
     7021 gggagcatct ggcggcgggc attcctttgg acgagtatct ggagcgcgta cccgacgagt
     7081 tttaccactg ggtgaaagct acctctgccg agttgcacaa ccgatttgaa gacattgaaa
     7141 atcaatgcaa agcagcattt aaaatcttcc cgaccagaaa agaaagcgcc gagtattttg
     7201 cccgctgtgc ttacccgaat gtgcttttca aaatgctgga cgggcggcct taccacgaca
     7261 ttatctggaa aatggtgagg ccggcgtttc aaaaagcgtt tacggcatcg aaagaatgaa
     7321 acagcttaaa actactcaca ccatgaaaaa agtcatcatc ctgcgcgggc tgccgggcag
     7381 cggaaaatcc accttcgccc ggcaattact ggcagaaacg cccggcgctt acaaacgcat
     7441 caaccgagac gatctgcgcg ccatgttcga caacgggcac tggtcgaaag gcaatgaaaa
     7501 attcgtcaaa caagtgcgcg acctgctcat tgtcagcgct ttgagcgagg gcaaacacgt
     7561 cattgtggac gataccaacc tggcgccgca caacgaaacc cgcatccggg aagtagtgcg
     7621 cgaatacaac cgcacgcaca acgattcggt gcaggtggaa ctgaaagaaa tggacacgcc
     7681 gctcgaagag tgcattgccc acgatgccct gcgcccgcaa cccgtaggcg cggcccgcat
     7741 ccgcgaaatg caccggcagt ttatggccgc cgctcaggct tcgtctatgc aacaggacga
     7801 gtcgttgccg cgcgccatca tctgcgacct cgacggcacc ctggctctgc tcaacgggcg
     7861 caatcccttt gatgcagcgc agtgcgaaaa ggatgtcctc aaccggccgg tggcggaagt
     7921 gctgaaaaga atgcgcgacg tgggctacaa gattttgctg ttgtcgggca ggcagtcgga
     7981 gcatcggcag cccacgctga actggctggc ccggcacgaa attccgttcg atatgctgga
     8041 aatgcgcgcc acgggagacg gacgaatgga cgccgtgatc aaaaaagagc ttttcgatca
     8101 gcacatccgg gggcggtttt acatcgaatt cgtattggac gaccgcaatc aggtggtgga
     8161 cttgtggcgg cgcgagctgg ggctgtcttg ctttcaggtg aattatgggg atttttagag
     8221 tagcctcgtc ttggacaagg ctactctaaa ctctataatg catggcaatt attttgccac
     8281 caccagcctg gcaggagggt atgaactgcc gggaaacacg atgtaataca ttccggcgct
     8341 gtatcgctcc aaacccattt ccagtatgcc cgtttgtgta atgccctgcg ctaccgacct
     8401 gccgtttaca tcggtcagaa gccagtgtac aggcttcgcc ggacggggag acagcacaat
     8461 gtttgtgttg atgccggcgg gattgggaaa aacaacaacc cgatgatttt cgcccggagc
     8521 cggctctttc gtacccaccg acagattgcc ctccccatcg gtgcgggcga gcaccgcatg
     8581 caagttccat accggcgggc ggagctgatt gtagccccac agcaaaattt gatcgggcgc
     8641 ggcgacgagt aaatcttctg cccattctgt gtttttagcg ccaaaatttc tcacccacag
     8701 aggctcggca ttttcgtcta ttttcagaag cataaagtca taaaagtggt cagtcgtgtc
     8761 ggccaatatt tcaccagcca cgaaatagcc gccccccgga gcgggtttga tactgtacgg
     8821 cgttgcgtag ttgaatgagc cgattacatg agttttaatc cactgaaaat cgccgttttc
     8881 atccaatttg ataaaaaaag gtatgctgct gaagatgccg ggaattactg ttgcccctgc
     8941 aaacataatg cctccatctg ctgtcggcgc aagtgcaaaa tccaacgtat atgtatcgta
     9001 ctccacgctc cacaagatgt tcccgtctga agctattttt tggaaatata tttgtgaatt
     9061 aatgtcggta ctttcgttag tgcctgtaaa aacaagctcg ccgccggata acgcaacagc
     9121 cttatagggt ttaaaggccg atcctgtaat ttcttcatac tgctgccaga tcacctctcc
     9181 cacatcattt attttgatat aaaattgatc gctgcctcga ttatcggcat tggctgtata
     9241 gccaaacatc acaaaatttc catccgtagt tggaacgatg ccgctcatcc aggagccgta
     9301 ctgctcgccg gccggacgaa tttttctatg ccagaggagt tggccggctg tatctaagcg
     9361 ggcaaggtaa gcatggccgt ttggcaaaat gtactctgcg cccgccggca ggtaattgcc
     9421 gaccacaaaa catccaccgt tgctcagagg cgccacaccg acaaaagtat ttcgtccttg
     9481 atctcccaag tctatggcat tcagccagat ggtgttgccg ttcggttcag ttttgaggat
     9541 caatccgtca aaactcgtcc ggtcgctgta actcattgtt tctccaacca gcagaacagt
     9601 tccatcggca gcttgggcca tttcgccacc aaaagtgaaa ttgcgggcgc cgaaaagtnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga cgggaaaaga
     9721 ttgagaatag ttgagaaacc cggaagccac aggaaatggg ccggcatcgt ctaaatatgc
     9781 ttttgcgtcc actttaaaaa tactgccggc accggcttcg gggggcgtat cggcataggg
     9841 ctgtatagaa aaccggaaca tggcgtcggc ttccgcagca gaatcgggga cgagatggag
     9901 gctgtccatt accacatgta caagcccgtt gtctgcgcgg gtaatgacag caggtctatt
     9961 ggaagctatg gggcgcaaag atgtaacgtc cagcgttgga ctgaatgtca aatga
//
LOCUS       DAORLF010000135         9884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3677_length_9884_cov_2.194018, whole genome shotgun sequence.
ACCESSION   DAORLF010000135 DAORLF010000000
VERSION     DAORLF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_9884_cov_2.194018"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PK198_12035"
     CDS             complement(<1..640)
                     /locus_tag="PK198_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRF39507.1"
                     /translation="MIANLIGATGLTGSSLLRQLLDDERFTVVRAFVRRPLYVTHAKL
                     ETHVIDFKQPDTWRHLVQGDVLFSTLGTTIREAGSQAAQYRVDFEYQYEFARAAADNG
                     ISTYILVSSVGANARSSNFYTRMKGELDEAVQQLDFQRIVILRPGFLDGKRKENRTGE
                     KIGLMVMRWIGRLPFLRPWRPIHTDIVAKAMIRGAMSTVPGKTIYTLGEVFEL"
     gene            complement(653..>1293)
                     /locus_tag="PK198_12040"
     CDS             complement(653..>1293)
                     /locus_tag="PK198_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRF39508.1"
                     /translation="EDYRQHFAMSLELLQAASSNDHFLPIPLGAQLPIFDGIRFRLGE
                     REYAFSGQAITPGAGASSREMLVKLARRYLHTPFLWGGRSPMGIDAAGLVQMAFKLCG
                     FRLPRTADKQVFEGENIDFTEQAQPGDVAFFENNLGRVVHCGIILPDNKIMHAFGKVR
                     IDLFDHYGVYDEESKKYSHRLRVIKSLLPPLVETPAAWKEKSEPAVNQIALF"
     assembly_gap    1294..1331
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1332..1561)
                     /locus_tag="PK198_12045"
     CDS             complement(<1332..1561)
                     /locus_tag="PK198_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF39509.1"
                     /translation="MENLVSLDALFSHNQLCKMTFAVCPLSVVAVRSHPSQRSEMVSQ
                     LLLGELVEIMEEKGRAWAKVRCVWDDLVGWAAT"
     assembly_gap    1622..1675
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1685..>2146
                     /locus_tag="PK198_12050"
     CDS             1685..>2146
                     /locus_tag="PK198_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF39510.1"
                     /translation="MLLQSNLLLGQDQHFTQYYASPMTLNPALTGAFSGTYRMSLIYR
                     DQWRNALTQPFATYSAAIDYRAQLKVKKKVLKDNVGVGVLFYNDRVPQTGFYHNQINV
                     AAAYHKSLNYRKNEFLTFGLQWGLNQRNFDYENFSFDDQFNGTNGYTLPGRE"
     assembly_gap    2147..2198
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2199..2716
                     /locus_tag="PK198_12055"
     CDS             <2199..2716
                     /locus_tag="PK198_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF39511.1"
                     /translation="HYTSAPDQGIGLYAGVAMHHILEPEISFYQAEENFLGIKTNRLR
                     RKYTAHAGLKIPLGERVQMLPRVLAYSQGPHLAVNAGSNFRFLVSEINGAAVHLGAWV
                     RPAKDEQNIMRIDALIGMVGFEYQSFLVGFSYDATVSYWTTAGRRTGAFELSLAYLGA
                     FENETVLCPKF"
     gene            complement(2720..>3650)
                     /locus_tag="PK198_12060"
     CDS             complement(2720..>3650)
                     /locus_tag="PK198_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRF39512.1"
                     /translation="RAEELGLDLMVSNNTALHETAFTVSIDLIGHGCTTGISAYDRAT
                     GIRAMLDPNIKSSDFARPGHIFPLRAKTGGVLRRTGHTEAAIDLARLAGFYPAGVLVE
                     ILNEDGSMARLPQLCAIAQKFGLKIISIKDLVAYRMRTERIVRRETEIQVNTQYGPFT
                     VRAFRQETTGDLHLAFCAGQWKEDEPVLVRVHSSSETGDMLGILFGNYGVMLEKSMQK
                     IGEEGKGVLLYMRHSEKSDAVLAHLNTMQQISPEEIRTQTITPDMAQRDFGVGAQILR
                     DLGITKIRLMTQNPKRRVGLIGYGLEIVEHISM"
     assembly_gap    3651..3664
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3665..3877)
                     /locus_tag="PK198_12065"
     CDS             complement(<3665..3877)
                     /locus_tag="PK198_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRF39513.1"
                     /translation="MSGNPPNATEAICLHTIEEAVEDIRAGKVIIVVDDEDRENEGDF
                     ICAAECITPEIINFMATHGRGLICTPI"
     assembly_gap    4022..4047
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4048..>4354)
                     /locus_tag="PK198_12070"
     CDS             complement(<4048..>4354)
                     /locus_tag="PK198_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF39514.1"
                     /translation="LVDDFFITMRAFERGAQAINEPLAVCHEPVSHEWREEYRRKARI
                     SAGNFQNMLLFRRLWWPPFTALSFAFFSHKILRWLGPFFLLGMALGMALVVWQGNYFS
                     "
     assembly_gap    4355..4367
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4368..>4966)
                     /locus_tag="PK198_12075"
     CDS             complement(<4368..>4966)
                     /locus_tag="PK198_12075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF39515.1"
                     /translation="AQGKTNNTNVFAPDDSNLPFVTVIMSLYNEEKVIGPKLETLLRQ
                     RYPTDKYNIIIGSDGSGDRTNEIVAQRVAGSASVQFFPFEQRRGKPEVINHLVRLALE
                     RRPAGPDHVFLVTDASVMLREEVVFHLAKHFRNPQIAVVDAFMTHTGLEDAGISRSED
                     RYISGEVRLKHLESIVWRKMIGPFGGCYALRSDYFSPVPS"
     assembly_gap    4967..4976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5059..6282)
                     /locus_tag="PK198_12080"
     CDS             complement(5059..6282)
                     /locus_tag="PK198_12080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF39516.1"
                     /translation="MRILQLCKKFPFPLKDGESIAVTGLSRALSGAGAEITLLAMNTS
                     KHYVEFVGKPRELEHYKEIHTVGVDNRIKPWDAFLNLFSQDSYHVSRFISPAFEQQLI
                     YLLQEEEYDVVQLETLYLAPYIPIIRKHSKALIVMRSHNVEHEIWERIVHNTGFPLKK
                     AYLSLLTRKLRRFEVQHLQEYDLLAAISQRDLNCFRALGYKNAAVVVPIGMDIKEYKP
                     DFKSYRKELSVSFIGSLDWRPNEEGVRWFLDDVWGPMQKKFGHLQLYIAGRNAPDWIR
                     KLKKRNIHFMGEVRSAARFINQHSIMVVPLLSGSGMRAKILEAMALGKVVISTTIGLE
                     GIPAKHRREVLVADTPDEFVEAVRYCQQQNGKLEQMGRRAVDFVTRHFDSAEVGRQLM
                     ETYSALLEESVKVRV"
     gene            complement(6361..>6818)
                     /locus_tag="PK198_12085"
     CDS             complement(6361..>6818)
                     /locus_tag="PK198_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39517.1"
                     /translation="TRIHGDYHLGQVLFDGRDFYIIDFEGEPMLSISERRLKRTPFKD
                     VAGMIRSFHYAAYGKLLLNANQRREDMPWLEQWARQWYHYVSRIFLSAYLDRVAGQPF
                     VPSDPEAARLLLRTYLLEKAIYEVGYEMNARPEWLRIPLKGVLFEMEQA"
     assembly_gap    6819..6843
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6844..9685)
                     /gene="treS"
                     /locus_tag="PK198_12090"
     CDS             complement(<6844..9685)
                     /gene="treS"
                     /locus_tag="PK198_12090"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRF39518.1"
                     /translation="MTPSTASLWYKNSIIYSLHVRSFYDSDGDGIGDINGLIEKLDYL
                     EDLGVDCLALMPFYPSPLRDDGYDVADYCNVHPECGTLEQFRRLLSEAHARNIRVVID
                     VLLNHTSTAHEWFQRARTSPPGSPERDFYVWSDSPERFAGAEVLFSDYESSNWEWDNV
                     AKAYYWHRFYSHQPDLNYDNPLVRSEIRKVFDFWFQLGVDGMRLTSVAFLFQREGTTS
                     ENLPEVHQFLRELRTHVDEHYPGKILIADTNLWPEAAAEYLGQGDQCHLNYHYPLMPR
                     MYMALQTEDRYPVVDILEQTPAIPDTTQWALFLRNHDDLTLSMVTEEERDYLYKVLAQ
                     DHGARINEGIRRRLAPLMGNDRRKLEIMYLLLFSLPGTPVLYYGDEIGMGDNIYLGDR
                     KGVRTPMQWNGDRNAGFSAANPQKLFLPVISDPQYRYEAVNVDVQNRNPASLLWWMKN
                     LIAVRKRSKAFSQGSIRFLNARNSKVLAFVRAFENESVLVLANLSKFSQAVQLDLSGY
                     EGIEPVEVFSQSRFQPVGEGGYPITLGPYGYYWFQLEKSESGEQDALERAIPELEIQG
                     DWQQVLEQYNYRRQLERRILPGYIRSNRWFGGKSRTAVSFELNRFPMVETEAGKAFLA
                     LIDVRYAEGLPDTYFLPLTLVTNAERMVHFLKNEPRSVVCYLKVGDTQGMLIDAIYEA
                     SFRNELFRLIRDNRSIEMPGGVLRFEAGKILTEEPSIPDDQVQSDVLRAEQSNTSVIY
                     NDQYFFKIYRKLDTDINPDLELVRYLSERTSFRNAPRYGGGIQYEERAGQSFTILGLL
                     QNKIPNQGEAWTAMLDTLGRYYEQVTAQTERSTPLPHLVHAERLYFDQAPELLQQLIG
                     SVTAERVVLLARRTAEMHMALAAEQIDPAFKPEHFTPHYQRSVYSAHRKLVSEKLDAL
                     EDKLPSFAPHVAAEARAVLDLREQILSTFSRIH"
BASE COUNT         2131 a         2595 c         2746 g         2180 t
ORIGIN      
        1 ccagttcaaa cacctcgccc agcgtgtaaa tggttttgcc gggaacggta ctcatcgcgc
       61 cgcgaatcat cgctttggct acaatatctg tgtgtatggg ccgccagggg cgcaggaaag
      121 gcagccgccc aatccagcgc atcaccatca gtccaatctt ctctccggtt ctgttttctt
      181 ttctttttcc atccaaaaac cccggcctca agatgacgat gcgctgaaaa tcaagctgtt
      241 gcaccgcttc gtccaattcg cctttcatgc gggtgtaaaa attggaactg cgggcattgg
      301 cgcccaccga agacactaat atataggtgg aaatgccgtt gtcggcggcg gcgcgggcaa
      361 attcatactg atactcgaag tccacccggt attgagcggc ctggcttccg gcctccctga
      421 tggtagtgcc gagcgtcgaa aaaagcacat cgccctgcac gagatgccgc caggtgtcgg
      481 gttgcttaaa gtcaatgacg tgtgtttcca atttggcgtg tgtgacgtac aagggccttc
      541 tcacaaaagc tcttactacg gtaaagcgct cgtcatcaag cagttgtcgg agcagggagg
      601 agccggtgag accggtagcg cctatgagat tggcgatcat ggatgttgat ttttagaaca
      661 aggctatctg attgacagcc ggttcggatt tttctttcca ggcagcggga gtttcgacta
      721 aagggggcag cagcgacttg atgacgcgga ggcggtgcga gtactttttg ctttcctcgt
      781 catagacgcc gtagtgatcg aagagatcaa tgcgcacctt gccgaatgcg tgcatgattt
      841 tgttgtcggg caggatgatg ccgcagtgga ctacccggcc cagattgttt tcaaaaaaag
      901 ccacgtcgcc gggttgcgcc tgctcggtga agtctatgtt ttcaccttca aaaacttgtt
      961 tgtcagccgt tctgggcaat cggaatccgc agagtttgaa tgccatttgc accaatccgg
     1021 cagcgtcaat gcccatcggc gagcggcctc cccaaaggaa gggcgtgtgc aggtagcggc
     1081 gtgccagttt gacgagcatt tcgcgggatg aagcgccggc gccgggcgtg atggcctgtc
     1141 cgctgaaggc atattcgcgt tcgccgaggc ggaaacgaat gccgtcgaag atgggcaatt
     1201 gcgcgcccag cggaatgggc aggaagtggt cgttggagga ggcagcttgc aggagttcga
     1261 ggctcatggc aaagtgctgc cgataatcct cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn ngtggcggcc cagcctacca ggtcatccca gacgcagcgc actttggccc
     1381 aggcccggcc tttttcttcc atgatttcga ccaattcgcc caagagcaat tgagaaacca
     1441 tttcactgcg ctgcgacggg tgcgagcgaa cagcgacaac actcagagga caaacggcga
     1501 aagtcatttt acaaagttgg ttgtggctaa agagagcgtc caaagatact aagttttcca
     1561 aaaactccgt ttttttgggg cttgttacac catttaccaa atcgaaatgt cgaaaatcag
     1621 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacact
     1681 tgtactgctg ctgcaatcca atttattact cggtcaggat caacacttta cccagtatta
     1741 cgcctctcca atgaccctca atccggcgct gacgggcgct ttcagcggca cctatcgcat
     1801 gtcgctcatt tatcgcgacc agtggcgcaa cgctcttacc cagccctttg ccacctattc
     1861 tgcggccatt gactaccggg cgcaattgaa ggtcaagaaa aaagtcctga aagacaatgt
     1921 aggggtgggc gtactttttt acaacgaccg cgtgccgcaa acgggctttt accacaatca
     1981 gatcaatgtg gccgccgcat atcataagtc gttgaattac agaaagaacg aatttcttac
     2041 tttcggtttg caatggggtt tgaaccagcg caacttcgat tacgaaaatt tcagtttcga
     2101 cgatcagttc aacggcacca acggctacac gctgccggga agggaannnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cattatacct cagcgcccga
     2221 tcaaggcatc ggactgtatg caggcgtggc catgcaccat attctggagc ctgaaattag
     2281 tttttatcag gcggaggaga attttttggg aatcaaaacc aatcgcctgc gcagaaaata
     2341 tacggctcat gcggggctga aaatccctct gggcgagcgg gtacaaatgt tgccaagggt
     2401 gctggcttat agccaaggcc ctcatttggc ggtgaatgcc ggctcgaatt tccgtttttt
     2461 ggtaagcgaa atcaatggcg cggcggtgca tctgggcgct tgggtgcggc cggccaaaga
     2521 tgagcaaaac atcatgcgga tagatgccct catcggcatg gtaggctttg agtatcagag
     2581 ttttctggta ggtttcagct acgatgctac cgtgagttac tggacgaccg caggccggcg
     2641 tacgggcgcg tttgaactct cgctggccta cctcggcgcg tttgaaaacg aaacggtgct
     2701 gtgcccgaag ttttgagaat tacatagaaa tatgctccac aatttccaag ccatacccaa
     2761 tcaaacccac ccggcgcttg gggttttgcg tcatcagccg gattttggta atgccgagat
     2821 cgcgcaggat ttgtgcgccg acgccgaaat cgcgctgcgc catgtcgggt gtaatggttt
     2881 gagtgcgaat ctcttccggt gaaatttgtt gcatggtatt gagatgcgcc aacaccgcgt
     2941 cgctcttttc gctgtgccgc atgtacagga gcacgccttt gccctcttcc ccgattttct
     3001 gcatggattt ttcgagcatc acgccgtaat tgccaaacaa aatgcccagc atatcgcccg
     3061 tttccgagga agagtgtacc cgcactaaca ccggctcatc ctccttccac tgcccggcac
     3121 aaaaggccaa atgcagatcg ccggtcgttt cctgccggaa tgcccgcacg gtgaacggac
     3181 cgtattgagt attgacctga atctccgttt ctctgcgcac aatgcgttcg gtgcgcatcc
     3241 ggtaggctac caagtctttg atggagatga ttttgaggcc gaatttctga gcaatggcgc
     3301 acaattgcgg caaacgcgcc atactgccgt cttcgttgag aatttccacc aaaacgccgg
     3361 caggataaaa ccccgccaac cttgccaaat ctatcgcagc ttcggtgtgg ccggtacgac
     3421 gcagcacgcc gccggttttg gcccgcagcg ggaaaatgtg tccgggccgc gcaaaatcgg
     3481 aggatttgat attcggatcg agcatagcac ggatgcccgt agcgcggtcg taagccgaaa
     3541 tgccggtggt gcagccatgc ccgatgaggt caatggacac cgtaaaggct gtttcgtgca
     3601 gggcggtatt gttcgatacc atcaggtcga ggcccaattc ctcggcgcgt nnnnnnnnnn
     3661 nnnngatggg cgtacagatc aaaccccggc catgcgtggc catgaagttg atgatttcgg
     3721 gcgtgatgca ctcggcggcg caaatgaaat cgccctcatt ttcgcggtct tcgtcgtcca
     3781 caacgatgat gactttgccg gcgcgaatgt cctcgacggc ttcttcgatg gtgtgcaggc
     3841 agatggcctc agtggcgttt ggtgggttcc cagacattgg atcgaattcc ttttaaaaac
     3901 ttgaaaaatc cttgaaacaa ggcaatgttc atcaataaaa agtaccgcaa attcctcagc
     3961 aaaaaaacat gcacgcccat gcgtttgagg gccacatcgg ccaatggcgc caagagtgct
     4021 gnnnnnnnnn nnnnnnnnnn nnnnnnngct gaagtagttg ccctgccaca caacgagggc
     4081 catgcccagc gccatgccca gcaagaaaaa cggccccagc caacgcagga ttttgtgcga
     4141 aaaaaaggcg aaagacaggg cggtaaacgg tggccaccac aggcggcgaa acagcagcat
     4201 gttttgaaaa ttgcccgccg aaatacgcgc cttgcgccgg tactcctcgc gccactcgtg
     4261 cgatacgggt tcgtgacaga cggccagggg ttcattgatg gcctgtgcgc cccgctcgaa
     4321 agcccgcatg gtgatgaaaa agtcgtccac cagannnnnn nnnnnnngaa ggcaccggac
     4381 tgaaataatc cgagcgcagg gcataacaac cgccaaacgg gccgatcatc ttgcgccaca
     4441 cgatgctttc caagtgtttg agccgtacct cgccggaaat gtagcggtct tcggagcgcg
     4501 aaatgcccgc gtcttcgagg ccggtgtggg tcataaaggc gtccaccacg gcgatttgcg
     4561 gatttctaaa atgtttggcc aagtgaaaaa ccacctcctc gcgcagcatc acgctggcat
     4621 cggtgacgag aaaaacgtga tcgggaccgg cggggcggcg ctccaaggcc aggcgcacca
     4681 aatgattgat gacttcgggt ttgccgcgcc tttgttcaaa cggaaaaaac tgcaccgatg
     4741 cgctgccggc cacccgttgc gccacgatct cattggtgcg gtctccagaa ccgtcggaac
     4801 cgatgataat attgtattta tcagtgggat agcgctgccg gagcagcgtt tcgagcttgg
     4861 ggcctatgac tttttcctcg ttgtacaagg acatgatgac ggtgacgaag ggcaggtttg
     4921 agtcgtccgg ggcaaagacg ttcgtgttgt tggttttgcc ttgggcnnnn nnnnnnaacc
     4981 atttcaacag cagcggatag aacacataag aatgtgccaa cgccaaaaaa gaaagggaaa
     5041 aaatgatcca gccaaccatc atacccgcac cttaacagac tcttcgagta atgccgaata
     5101 cgtctccatc aactgccgcc ccacctcggc actgtcgaaa tgccgcgtca caaaatccac
     5161 agcgcggcgg cccatctgct ccaacttgcc gttttgctgc tgacagtacc gaacggcctc
     5221 cacaaactca tcgggcgtat cggccaccaa aacctcgcgg cggtgtttgg caggaatgcc
     5281 ctccaaacca atggtagtag aaatgacgac cttgcccaat gccatggctt ccaatatctt
     5341 agccctcatg ccgctgcccg acaagagggg caccaccatg atgctgtgct gattgatgaa
     5401 ccgcgccgcg ctgcgcactt cgcccatgaa atggatgttg cgctttttga gtttgcgaat
     5461 ccagtcgggg gcgtttctgc cggcaatgta gagttgcaaa tggccgaatt ttttttgcat
     5521 cggcccccac acatcgtcta agaaccagcg cacgccttcc tcgttgggac gccagtccaa
     5581 agaaccgata aagctgacgc tcaattcttt gcgatagctc ttgaaatccg gcttgtactc
     5641 tttgatgtcc ataccgatgg gcaccaccac cgctgcattc ttgtagccca aggccctgaa
     5701 acagttcaga tcgcgctgag aaatggccgc cagcagatcg tattcctgaa ggtgctgcac
     5761 ctcgaaacgt ctgagtttgc gggtcagcag cgagaggtag gcttttttga gcggaaatcc
     5821 ggtattgtgt acgatgcgct cccagatttc gtgctccaca ttgtgcgaac gcatgacaat
     5881 caaggctttg gaatgcttgc ggatgatggg gatgtacggc gccagataca gcgtttccaa
     5941 ctgcaccacg tcgtattctt cttcctgtaa cagatagatg agttgctgct caaaagccgg
     6001 cgagatgaag cgggaaacat ggtacgaatc ctgagaaaag agattgagaa aagcatccca
     6061 aggcttgatt cggttgtcca cgcccaccgt atgaatttct ttataatgtt ctaattctct
     6121 gggttttccg acaaactcta catagtgttt gctcgtattc atggccagca gcgtaatctc
     6181 cgcgcctgcc ccgctcaatg ccctgctaag ccctgttacc gcaatggatt cgccatcttt
     6241 taacggaaat ggaaattttt tgcacaattg tagtatcctc atgcgggttg tattgcaggg
     6301 aggcaaaaat aaacttttgt tccactattg taatacatac aggcaagttt tgtgttcgcg
     6361 tcaggcttgt tccatttcaa aaagaacgcc tttgagcgga atgcggagcc actccgggcg
     6421 ggcattcatt tcataaccaa cctcatagat ggctttttcc aacaggtagg ttcgcagcag
     6481 caatcgggcg gcttcggggt cggaaggcac aaaaggctgc ccggccacgc ggtctaaata
     6541 cgcgctcagg aaaatccggc tcacataatg ataccactgc ctcgcccatt gctccaacca
     6601 gggcatgtcc tcgcggcgtt ggttggcgtt gagcaacaac ttgccgtaag ccgcatagtg
     6661 aaatgagcga atcatgccgg ccacatcctt gaacggcgtg cgtttgagcc tgcgctcgct
     6721 gatggaaagc atgggttcgc cctcaaaatc aatgatgtag aaatcgcggc cgtcgaacag
     6781 cacctgcccg aggtggtaat cgccgtgtat gcgggtttnn nnnnnnnnnn nnnnnnnnnn
     6841 nnncgtggat gcggctaaac gtggagagga tttgctcccg gaggtccagc acggcgcggg
     6901 cctcggcggc tacatgcggc gcaaaagagg gcagtttgtc ttccaatgcg tccaattttt
     6961 cgctcaccaa tttccggtgc gccgaataca cagaccgctg gtaatgaggc gtaaagtgct
     7021 ccggtttgaa ggcaggatcg atctgctcgg cggccaatgc catgtgcatt tcggcggtgc
     7081 gccgggccag caacacgacg cgctcggcgg tgacgctgcc gatgagttgt tgcagcagtt
     7141 cgggcgcctg atcgaagtac aaacgctcgg catgaaccag atggggtagg ggagtgctgc
     7201 gctcggtttg ggccgtcacc tgttcgtagt agcggcccag ggtgtcgagc atggcggtcc
     7261 aggcttcgcc ctgattgggg attttgtttt gcaacaatcc cagaatcgtg aacgactgcc
     7321 cggcgcgctc ctcgtactgt atgccgccgc cgtatcgagg cgcgttgcgg aaggaggtgc
     7381 gctccgatag gtatctcacc aactccagat cggggttgat gtcggtgtcc aacttgcggt
     7441 atattttgaa aaaatactgg tcgttgtaaa tcaccgaagt gttgctctgc tcggccctca
     7501 gcacatccga ctgaacctga tcgtcgggga tggagggttc ctcggtcagg attttgcccg
     7561 cctcgaagcg cagcacgccg ccgggcattt ctatcgaacg gttgtcgcga atgagccgga
     7621 acaactcgtt tctgaacgat gcctcgtaaa tggcgtctat gagcatgccc tgcgtgtcgc
     7681 ccactttgag gtaacagacc acgctgcgcg gctcgttttt caaaaaatgc accatgcgct
     7741 cggcattcgt taccaaggtc agcggcagga agtaggtgtc gggcaggccc tcggcgtagc
     7801 gcacgtctat cagcgccaga aaggctttgc cggcctcggt ttccaccatc gggaagcggt
     7861 tgagttcgaa ggaaaccgcc gtgcgcgact tgcctccaaa ccagcgattg gaacgaatgt
     7921 aacccggcag aatgcgccgc tccaattgcc gccggtagtt gtactgctcc agcacctgct
     7981 gccagtcgcc ctgtatttcc aactccggta tggcgcgctc caaagcatcc tgctcgccgc
     8041 tctcgctttt ttccaactgg aaccaatagt aaccatacgg ccccagcgtg atggggtagc
     8101 cgccctcgcc cacgggctgg aaccggctct ggctgaacac ctccaccggc tctatgccct
     8161 cgtagccgct caggtccaac tgcaccgcct gcgagaattt cgagaggttg gccagcacca
     8221 gcacgctttc gttttcaaag gcccgcacaa aggccagcac cttgctgttg cgggcgttga
     8281 ggaatcggat gctgccctga ctgaaggctt tggagcgctt gcgcacggca atgaggtttt
     8341 tcatccacca cagcagcgag gccggattgc ggttttgcac gtccacgttg acggcctcgt
     8401 agcggtactg cgggtcgctg atgacgggca ggaacagctt ttgcgggttg gccgccgaga
     8461 agcccgcgtt gcggtcgccg ttccattgca tgggggtgcg cacgcctttg cggtcgccga
     8521 gatagatgtt gtcgcccatg ccgatttcgt cgccatagta cagcacgggc gtgccgggca
     8581 gcgaaaacag tagcaggtac atgatttcca acttgcggcg gtcgttgccc atcagcggcg
     8641 cgaggcgtcg gcggatgccc tcgttgatgc gggcgccgtg atcctgggcc agcaccttat
     8701 agaggtagtc gcgttcttcc tccgtcacca tcgagagggt gaggtcgtcg tggttgcgca
     8761 ggaagagcgc ccactgggtg gtgtcgggaa tggccggcgt ttgttccagt atgtccacca
     8821 ccgggtatcg gtcttcggtt tgcagtgcca tgtacatgcg cggcatcagc gggtagtggt
     8881 agttgaggtg gcattggtcg ccctgaccga ggtattcggc ggcggcttcg ggccagaggt
     8941 tggtgtcggc aatgaggatt ttgccggggt agtgctcgtc cacatgcgtg cgcagctcgc
     9001 gcaggaactg gtgaacttcg ggcaggtttt cgctggtagt gccctcgcgc tggaacaaaa
     9061 acgccaccga ggtgagccgc atcccgtcca cgccgagttg gaaccaaaaa tcaaacacct
     9121 tgcgtatttc gctgcgtacc aatggattgt cgtagttcaa atccggctga tggctgtaaa
     9181 accggtgcca gtagtaggct ttggccacat tgtcccattc ccagttggaa ctctcgtaat
     9241 cggaaaacaa gacctccgcg ccggcaaagc gttcgggact gtcgctccac acataaaaat
     9301 ccctttcggg cgatcccggc ggcgaggttc tggcccgctg aaaccactca tgtgccgtag
     9361 aagtgtggtt gagcagcaca tctatcacca cgcggatgtt gcgggcatgg gcctccgaca
     9421 gcaggcggcg aaactgctcc aaagtgccac attcgggatg cacattgcaa tagtcggcca
     9481 catcgtagcc gtcgtcgcgc aggggcgagg gataaaacgg catcagggcc agacagtcca
     9541 cgccgaggtc ttccagatag tccaattttt caataaggcc gttgatgtcg ccgatgccgt
     9601 cgccgtcgct gtcgtaaaac gagcggacat gcagcgaata gatgatgctg tttttgtacc
     9661 aaagcgaagc agtagaaggc gtcatggcgc tgtgttttgt gtgctgtctg atttcgggcg
     9721 taatgttacg aaaagtgtgg gtgtggtgca agtacggggt ctgaagcgcg tgcagattcc
     9781 ggcgaggagg gggagttttt tgggtagggg gtgttggagg tttggggaga gggtggtatc
     9841 tttgcaggag aggggggatg ctttacttta tatccgtctg gaga
//
LOCUS       DAORLF010000136         9875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3680_length_9875_cov_1.850305, whole genome shotgun sequence.
ACCESSION   DAORLF010000136 DAORLF010000000
VERSION     DAORLF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_9875_cov_1.850305"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>303
                     /locus_tag="PK198_12095"
     CDS             <1..>303
                     /locus_tag="PK198_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39519.1"
                     /translation="NERTAAVVVEPVQAEWGVRVPFNDYLLKLRRRCDETGALLILDE
                     VQTGFGRTGTFFAFEQYGIVPDILLLAKGMGGGMPIGAFIASRHIMQALSHQPILGH"
     assembly_gap    304..421
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <422..650
                     /locus_tag="PK198_12100"
     CDS             <422..650
                     /locus_tag="PK198_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF39520.1"
                     /translation="PAIRELRNAGLLMAVELADFETLQQVIRRCLDKGLLTDWFLFNN
                     RSLRIAPPLIINREEIEWACGIILEALEETV"
     gene            complement(667..1101)
                     /locus_tag="PK198_12105"
     CDS             complement(667..1101)
                     /locus_tag="PK198_12105"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRF39521.1"
                     /translation="MNKDSFIEAAIEEAQKGLAEGGIPIGSVLVHRGRIIGRGHNRRV
                     QQGSAILHGEMDALENAGRLPASVYKECVLYTTLSPCSMCSGAILLYGIPKVVIGENT
                     TFMGEEGLLSARGVDVEVVHDPTCVALMQQFIADKPALWRQS"
     gene            1257..2606
                     /locus_tag="PK198_12110"
     CDS             1257..2606
                     /locus_tag="PK198_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF39522.1"
                     /translation="MKHFSLLVALLLSSVAFSQKTVTLTCKVDACQAPLRLFKFDGLL
                     FKDAQISPVTSPDGSITFAVPGGKPQFYYLGFRSDQTLILILGAEKDVRVEGSCNDIR
                     TATISGSPLNDSYQWVKNRVNIFKNQTGQLIQQYQAAMGNQQQTQQVTAQMKALDDQK
                     LQLLDSLQRTQPYLAKIAALNTYLSWPNSQAGYSNEIEYFAKAFFKYADFKDEGYEGL
                     PWVFEAFQGYASTLVNVGLDDATQKVYFEQMLEKIPAGTMRSRMALAGIIGPLKQKNS
                     PNYVVFAERLLKELKAGDEAAIANLQQQVAGMRSFTIGAEAPDFAQATPEGKMLKLSE
                     LRGKVVLIDFWASWCGPCRRENPNVVRMYQQYKDKGFEILGVSLDRDRQRWLDAIQQD
                     GLTWLHVSDLQQWSNAVAQMYGVSSIPHTVLLDAEGRILARNLRGEALEHKLAEIFK"
     gene            2610..3278
                     /locus_tag="PK198_12115"
     CDS             2610..3278
                     /locus_tag="PK198_12115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF39523.1"
                     /translation="MPPIPRWKRLLSYFFEFHIESAPSDLNPHLYVSLNRGRYQLSTA
                     NAVYSFADLYDNFSKAFQRLQPAQLPINEVLLLGFGLGSIPLMLEKKFHCRFRYTGVE
                     ADESVIWLAGKYALPELESPIELHCADAYAYVFSTEERFDLICMDVFLDDQVPEEFTQ
                     TDFLEALRDLLLPGGVLLYNRLSATEEDRSLSESFWKNAFLKVFPQGDRWDFDGNWIL
                     KAQV"
     gene            complement(3461..5809)
                     /locus_tag="PK198_12120"
     CDS             complement(3461..5809)
                     /locus_tag="PK198_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF39524.1"
                     /translation="MKQKTYLILALALLMSTGLAFQSLAQGCSDLFFSEYVEGTSFEK
                     YIEIYNPTSGTVDLSDYQLRLYTNGSATPSQTMPLSGSLASGAVIVVRNGSATGFTGG
                     VVNSNVINHNGDDAFDLYKISAAAAVDIFGRIGDDPGTAWTATGLSTLDRTLRRKFTV
                     TGGVTVNPSGTGASAFTTLATEWDGYLVNDVSGLGSHTSNCISTACSISSIMLSNVSG
                     CNDNGTPLVSGDDYFTADVVVSYTGAPTTDSLVLMGSTFRGAVPVGSIGASSYTFTGV
                     QFAANGLPISLTAFFKADGACTLTNLSAGIAPASCSTPAPCTLPFFSEYIEGSGNNKC
                     LEIYNPTASPIDLTAGGYTIKMYFNGSGSAGLTINLTGIIPAGGTYVVCNSSSTAEFT
                     ALANQIGSGGWFNGDDAVSLENSSGVLDIIGQIGTDPGSAWTSAGVSTVDQTLRRYNY
                     ISKGDNNGTDAFDPSLEWASYPINTSAGLGYHISTCPTSVPAGWATGNPGCAAGSTSV
                     ASGTWTQTSNCYNAAPGADDLTYAFQEMCGDATIIAQYVGVTPFGFAGLMMRESLNPG
                     SKYVWMFMRANTNASWSIRSITGLTPTFDTKPHLNRTWMKLTRSGSLFRGYLSTNGTT
                     WQLVFQSSVSLNSCVLVGLATHSNVDGSNVTATFRNVSVSSGANLMALPGAAQAVDMV
                     SEAPAMQTSVIQSNNSDNRDAFGIWPNPAGNTLEVNIPVWNTTESVQLQVNDMQGRPL
                     MYRQIGDQQQRATLDLSSLAPGVYLLTLRSGEHLEIARFVKQ"
     gene            complement(6020..6841)
                     /gene="prmC"
                     /locus_tag="PK198_12125"
     CDS             complement(6020..6841)
                     /gene="prmC"
                     /locus_tag="PK198_12125"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRF39525.1"
                     /translation="MTAQLSARFGEGEARAMSRLIWEAIFQKRLLPAADLELSAEQSE
                     KLAGITTRILDGEPIQYVLEHTWFYGLPFRTDSRALIPRPETEELVHWILETNGPATR
                     LRVLDIGAGSGCIAVTLKKKRPLWDVTALDVSEAALALTLENARLHDCSIDTILQDML
                     DPTQWLQWGQWDIIVSNPPYIPQNERRLMPEQVLRHEPESALFVPDDKPLLFYEAISR
                     FAMSHLESNGALYLEINEFYAQQTQAYLRADDWRDVRLQPDLQGKMRMIRACKHC"
     gene            complement(6875..>8014)
                     /gene="nspC"
                     /locus_tag="PK198_12130"
     CDS             complement(6875..>8014)
                     /gene="nspC"
                     /locus_tag="PK198_12130"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRF39526.1"
                     /translation="NNEYASLRSAAYVLEERKLRENLELIQRVQSEAGVFIILALKGF
                     AMWSVFPMVKRYLSGATASSLHEARLIFEEMGCKAHTYAVAYMPDEFDEIMGYSSHIT
                     FNSIAQYKRYAAAVESYPEKISPGIRVNPEYSEVEVALYNPCAPGSRLGEVSDAFYAG
                     LPEGVEGLHFHTLCESSSYDLEKVLAAFEERFGRFLPQLKWVNFGGGHLMTRKGYDTE
                     HLIALLRAFRARHPHLEVILEPGSAIAWETGVLVAHVLDIVENKGIQTAILDVSFTAH
                     MPDTLEMPYRPRIRGAADRTPGKPTYRMGGVSCLSGDYMLEYSFENPLQPGDQIILED
                     MIHYTMVKTTTFNGVRHPDICILRETGEIETVKRFSYEDYRNRLS"
     assembly_gap    8015..8138
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8301..>8818)
                     /locus_tag="PK198_12135"
     CDS             complement(8301..>8818)
                     /locus_tag="PK198_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRF39527.1"
                     /translation="LLDTGVDVLKIEGRARAPEYVKAVTRAYREAINAVQNGDFSAEK
                     VDAWMQEVEKVYNRGFWGGYYLGKQLGEWADSYGSKATTKKIYLGRGVHYFPKVQVGE
                     FKIESHTLKKGDSLLVTGPATGVLEFEADEIRVDDQTVDSARQGVSCSLKVPAKIRPS
                     DKLYKIVDAEA"
     assembly_gap    8819..8828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8829..>9316)
                     /locus_tag="PK198_12140"
     CDS             complement(<8829..>9316)
                     /locus_tag="PK198_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRF39528.1"
                     /translation="MKRIVDEAVKHNISAVIASDQAVIGYAASKGLPVHISTQVNISN
                     IETLKFYAHFADVMVLARELSMVQMREITEAIQRDQITGPSGQLVRIEVFAHGALCMA
                     ISGKCYLSLHTHNSSANRGVCIQNCRRQYEVTDRDEGHAFHIENEYIMSPKDLNTLPF
                     LD"
     assembly_gap    9317..9343
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9344..9544)
                     /locus_tag="PK198_12145"
     CDS             complement(<9344..9544)
                     /locus_tag="PK198_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRF39529.1"
                     /translation="MVKKHIELMAPAGNFESMMAAIQGGADSVYFGIEQLNMRARATM
                     NFTLDDLEAVSKLCKEHGVKSYI"
     gene            9617..>9875
                     /locus_tag="PK198_12150"
     CDS             9617..>9875
                     /locus_tag="PK198_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRF39530.1"
                     /translation="MSAQRTLEICVESVTSALNAQTGGADRIELCDNLHQGGITPGAG
                     KIVLAKKLLHIPVFVLIRPRKGDFLYSDIEFDTLLEDIALAR"
BASE COUNT         2278 a         2611 c         2438 g         2269 t
ORIGIN      
        1 aacgagcgca ccgctgccgt cgtcgtagaa cccgtgcagg ccgagtgggg cgtgcgcgta
       61 cccttcaatg actacctgct gaaattgcgc cgtcgctgcg acgaaaccgg cgctctgctc
      121 atcctcgacg aggtgcaaac gggttttggg cgcacgggca ctttttttgc gttcgagcag
      181 tacggcatcg ttcccgatat tttgctgttg gccaaaggta tgggtggcgg tatgcctatc
      241 ggagctttca ttgcgtcccg gcacatcatg caggcgctca gtcaccagcc tatcttgggg
      301 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 ncccggccat tcgggaactg cgcaatgccg gcctgttgat ggctgtggaa ctggctgatt
      481 ttgagacgct tcagcaggtt attcgccgat gtttggacaa gggtttgctc accgactggt
      541 ttttattcaa caaccgttcg ctccgcatag cgccgcccct catcatcaac cgagaggaga
      601 ttgaatgggc ttgcggaatc attttagagg cattagaaga aacggtgtaa ttattcgccg
      661 atgtcttcat gattgtctcc aaagcgccgg cttgtcggcg ataaactgct gcatcagcgc
      721 aacgcaggta gggtcatgca ctacttcaac gtccacgccg cgcgccgaaa gcagtccttc
      781 ctcgcccata aaagtggtgt tttcacctat cacgactttc ggaattccat acagcaaaat
      841 agcgccgctg cacatgctgc aaggcgacaa cgtagtatat aaaacgcact ctttgtaaac
      901 cgaagccggc aatcgccctg cgttttctaa tgcgtccatt tcgccgtgca ggatggcgct
      961 gccctgttgc accctgcggt tgtgtccccg cccgatgatg cgcccccgat gcaccaacac
     1021 actgccgatg ggaatgccgc cttcggccaa tcctttttga gcctcttcaa tggcggcctc
     1081 tataaaactg tctttattca tgtctaatgc gtttaaacag gctctgaaca ctcccaaacc
     1141 gcgcatcgtt cagccggccg cattgagttg gccaaatact ttgttgcaaa actcctccgt
     1201 tgattatgcc gttaataatc ttacttttgc accgtcaaaa aaacaactca ttcgctatga
     1261 agcatttttc tctgcttgtc gcactcttgc tctcctctgt tgcattttct caaaagaccg
     1321 tcacgctcac ttgcaaagtg gatgcctgtc aggcgccatt gcggcttttc aagtttgatg
     1381 gcctgttgtt caaagatgcg caaatcagcc ccgtcacttc gcccgacgga agcatcacat
     1441 ttgcggtgcc cggcggaaaa cctcagtttt actacctcgg cttccggagc gatcagacgc
     1501 tgattctcat tttgggcgcc gaaaaagatg tgcgtgtgga aggttcctgc aacgatatcc
     1561 gaactgctac catctccggc tcaccgctca acgacagcta ccaatgggta aaaaatcgcg
     1621 tcaatatttt taaaaatcaa accggccagc ttatacaaca gtatcaggcg gctatgggca
     1681 atcaacagca aacacagcag gtgacggctc aaatgaaggc tttggacgat cagaagctgc
     1741 aactcttgga ttcgctccaa cgcacgcagc cgtatctggc aaaaattgcc gcactcaata
     1801 cctacctcag ttggcccaac agccaggctg gctactccaa tgaaatcgag tattttgcca
     1861 aagcattttt caaatatgcc gactttaaag atgaaggcta tgaagggctg ccctgggttt
     1921 ttgaagcatt tcaggggtac gcctctacct tggtcaatgt gggcttagac gacgccacac
     1981 agaaggtata ttttgaacaa atgctcgaaa aaatacctgc cggaaccatg cgctccagaa
     2041 tggctttggc gggcatcata gggccgctca aacaaaaaaa cagccccaat tatgtggtct
     2101 ttgccgagcg tttgctcaaa gaactcaaag ccggagacga agccgctatt gccaaccttc
     2161 agcagcaagt tgccggtatg cgcagtttca ccatcggagc tgaagcgccc gattttgcac
     2221 aagcaacccc ggaaggcaaa atgctcaaat tgagcgaatt gcgcggaaaa gtagtgctca
     2281 ttgacttctg ggcaagctgg tgcggcccct gccgccggga aaaccccaac gtggtgcgca
     2341 tgtatcagca gtacaaagac aaaggctttg aaatattagg cgtaagcctc gaccgcgacc
     2401 gccagcgctg gctcgatgcc atccaacaag acggacttac ctggctgcac gtcagcgatt
     2461 tgcagcagtg gagcaatgcc gtagcgcaga tgtacggtgt atcgtccatt ccgcataccg
     2521 tactgcttga cgcagaaggc cgcattctcg cccgcaactt gcgcggcgaa gcgctggaac
     2581 acaaattggc cgaaattttc aaataaacca tgccgcccat tccgcgctgg aaacgcttgt
     2641 tgagctactt ttttgaattc catattgaga gcgcgcccag cgatctcaat ccgcacctgt
     2701 atgtgagtct caatcggggc cggtatcagt tgagtactgc caatgccgta tattctttcg
     2761 ccgatttgta cgataatttc tcgaaggctt ttcagcggct tcaaccagca caacttccta
     2821 tcaatgaggt actcctgctg ggctttggct tgggcagtat tcccttgatg ctggaaaaaa
     2881 aattccactg ccgatttcga tataccggcg ttgaggccga tgagtctgtc atctggttgg
     2941 ccggcaaata cgcgctgccc gaattggaat cgcccataga actgcattgc gcagacgcct
     3001 acgcttatgt tttcagcacc gaagagcgct ttgatcttat ttgcatggac gtgtttttgg
     3061 atgatcaagt gccggaagaa ttcacacaaa ccgattttct ggaggctctg cgcgacctgc
     3121 ttttgcccgg cggcgtattg ctctacaacc ggctcagcgc taccgaagag gacaggtctc
     3181 tatcggaatc attttggaaa aacgcctttc taaaagtttt tccacagggc gatcgttggg
     3241 atttcgacgg caactggata ttaaaagctc aggtgtaata aaaaaagccc ttgattcgac
     3301 agaggccggc tcaagagctt agataaatga aaacactttg agaataaacg aacgaaagaa
     3361 gattttgttg atgctccata aaattgcatg aaactttgcc aaatcaaaaa agccgccccc
     3421 tgtatcggaa gcggctcttt tgattcatac tactcttttc ttattgcttg acaaacctcg
     3481 ctatttcgag gtgttcgccc gaacgcaaag tgagcaaata caccccaggc gccaaagacg
     3541 acaaatcgag tgtggcgcgc tgctgctgat cgccgatctg gcggtacatt agcggccttc
     3601 cctgcatgtc gtttacctga agctgcacag attcggtcgt attccagaca ggaatgttca
     3661 cttccagcgt gttgccggcc gggttgggcc agatgccgaa cgcatcgcgg ttatccgaat
     3721 tatttgactg aatcacagat gtttgcatcg ccggcgcctc cgaaaccatg tccacagcct
     3781 gagcagcgcc cggcagagcc atcaggttgg cgccggaaga cacgctcacg tttctgaacg
     3841 tagccgtcac attgcttccg tccacattgc tgtgggtagc caatccgacc aatacacagc
     3901 tattgaggct caccgagctt tggaacacca actgccaggt ggtgccgttc gtggagaggt
     3961 agcccctgaa taagctgccg gagcgggtca gtttcatcca ggtgcggttc agatgaggct
     4021 tcgtgtcgaa tgtgggcgtc aacccggtaa tagagcgaat ggaccaagac gcattggtat
     4081 tggcacgcat gaacatccaa acgtatttag aacccggatt cagcgattcg cgcatcatca
     4141 ggcccgcaaa gccaaaaggc gtaacaccga catactgcgc aataatggtg gcatcgccgc
     4201 acatttcctg aaaagcatac gtcagatcat cggcgccggg agcggcattg tagcagttgg
     4261 atgtttgcgt ccaagtaccg ctcgctaccg aggtggagcc tgctgcacag cccggattgc
     4321 ccgtcgccca gcctgcgggt acgctcgtgg ggcaagtgct gatatgatag cccagacctg
     4381 ctgaggtatt gatgggataa gatgcccatt cgaggctggg gtcaaacgca tccgttccgt
     4441 tgttgtctcc tttggaaatg taattgtaac gacgcagcgt ctggtccacc gtagaaacgc
     4501 cggcactcgt ccaggcgctt ccggggtcgg taccaatctg gcctataatg tcgagaactc
     4561 cggaagagtt ttccagcgat acggcatcgt cgccattgaa ccacccgcca ctgccgatct
     4621 gattggccaa tgccgtaaat tcagccgttg aactgctgtt gcaaaccaca taagtacctc
     4681 ctgcgggaat gatgcccgtg aggttgatgg tgagacctgc actgcccgat ccgttgaagt
     4741 acattttaat ggtatatcct ccggcagtca gatcaatcgg actcgccgtc gggttgtaaa
     4801 tttcaaggca tttgttgttg ccgctgccct cgatgtattc agagaagaaa ggcagagtgc
     4861 agggcgcagg cgtagaacaa gatgccgggg caatgcctgc actcaggttg gtaagcgtgc
     4921 aggccccgtc ggccttgaaa aatgcagtaa gggatatcgg caggccgttg gcggcaaatt
     4981 gaacaccggt aaacgtgtag gaagaagcgc cgatagagcc taccggaaca gcccctctga
     5041 acgtgctgcc catcaatacc aaagagtcgg tggtgggagc gccggtatag ctcaccacaa
     5101 catcggcagt gaagtagtcg tcaccactga ccagaggcgt gccgttatcg ttacaaccgg
     5161 acacattgct cagcatgata gaagaaatag agcaggccgt agaaatacag ttactggtat
     5221 gactgcccaa gccggatacg tcgttgacga gatagccgtc ccactccgta gccagggtgg
     5281 taaatgccga tgctcctgtt cctgaaggat ttacagtaac accccccgtg actgtgaact
     5341 tcctgcgcaa agtgcgatct aatgtggaca atcccgtagc ggtccacgca gtaccggggt
     5401 cgtctccaat tcttccaaaa atatctacgg cggcggcggc agaaattttg tacaaatcaa
     5461 acgcgtcgtc gccattgtga ttgatgacat tggaattgac tacaccgccg gtaaatcccg
     5521 tagcgctgcc attccgtacc acaatcaccg ccccgcttgc caagctgccg ctcaggggca
     5581 tggtttgtga tggcgtggcc gaaccattgg tataaagccg caattggtaa tccgacaagt
     5641 caaccgtacc ggatgtgggg ttataaatct cgatgtactt ctcgaaactc gttccttcta
     5701 catattccga aaagaaaaga tcggagcacc cctgcgccaa tgattgaaag gccaagcctg
     5761 tgctcatcag caaggcgagg gctaaaatca ggtaagtttt ctgtttcata atgaaagcgt
     5821 tattgatgaa atgaatggac aaaacataca catgatgttg gtatgcagca tctcaaaagc
     5881 tcggaataaa tgtaagagga aatgcaacga gggggaattg cattatttgt aaatagcggc
     5941 tcaaaaatac atacttacat caaactcgca agtttttttt gcaaaaaaag cctgccgaac
     6001 atcaaaattt aatcagcaat taacaatgct tgcaggcgcg gatcatgcgc atcttgcctt
     6061 gcaggtccgg ctgaagtcgc acgtcccgcc aatcgtcggc acggagatac gcttgggttt
     6121 gttgtgcata aaactcattg atttccagat acaaagcccc gttactctcc aagtgcgaca
     6181 tagcaaagcg ggaaatagct tcgtaaaaaa gcaagggctt gtcgtccggt acaaacaagg
     6241 cggattccgg ctcgtggcgc agcacttgct ccggcatcag gcggcgttca ttttggggaa
     6301 tgtacggcgg gttgctcaca atgatatccc attggcccca ttgcagccat tgcgtgggat
     6361 cgagcatgtc ttgcaaaatg gtatctatag agcaatcgtg caggcgggca ttttcaagcg
     6421 tcagggccaa tgcggcttcg gagacatcca atgccgtcac atcccaaagg ggtcgttttt
     6481 tcttcagcgt taccgcaatg cagccgctgc ccgcgccaat gtccaacacg cgaagccggg
     6541 tagcagggcc gtttgtttcc aaaatccaat gcaccaactc ctcggtttcg ggacggggaa
     6601 tcaaggctcg cgaatcggtt cggaatggca gcccgtaaaa ccaggtgtgc tccaacacat
     6661 attgaatcgg ttctccgtcg agaatgcgcg ttgtaatacc ggctaatttt tccgattgtt
     6721 cggcactgag ttcaaggtcg gcggcaggca acagccgttt ttggaatatc gcttcccaaa
     6781 tcagccggga catagcccgc gcctcgccct cgccgaaacg ggcactcagt tgtgccgtca
     6841 aatttgcctt agcctgcccg tattgcatag aaaatcagga gagcctgttg cgataatctt
     6901 cgtaagaaaa cctcttcacc gtttctatct cgccggtttc ccgcaggatg cagatgtcgg
     6961 gatgccgcac gccgttgaac gtggtggttt tgaccatcgt gtagtgaatc atgtcttcca
     7021 gaatgatctg atcgccgggt tgaagcggat tttcaaaact gtactccagc atgtaatctc
     7081 ctgacaaaca gctcactccg cccatacgat aagtgggttt tccgggcgtt cggtcggcag
     7141 cgccccggat gcgcgggcga tacggcattt ccagggtgtc gggcatgtgc gcagtaaaag
     7201 acacgtcgag gatggcggtt tggatgcctt tgttttcaac gatatccaaa acgtgggcca
     7261 ccaaaacgcc ggtttcccag gcaatggcgc tgcccggttc gaggatgacc tccagatgcg
     7321 gatgcctcgc ccgaaatgcc cgcaacaacg cgataaggtg ctcggtatcg tagcctttgc
     7381 gcgtcatcag gtgcccgccg ccgaaattca cccatttcaa ttgcggcaaa aaccgcccga
     7441 aacgctcctc aaaagcagcc agtacttttt ccaaatcata cgaactcgat tcgcacagcg
     7501 tatgaaaatg aagcccttcc acgccttccg gcaaaccggc ataaaaagcg tccgacactt
     7561 cgcccaaacg ggaacccggc gcgcagggat tgtacagcgc tacttctacc tccgagtatt
     7621 cagggttcac ccggatgccg ggcgatattt tttccgggta gctctccaca gcagcggcgt
     7681 agcgcttgta ttgggcaatg gaattgaagg taatatggct gctgtagccc atgatttcat
     7741 caaactcgtc gggcatgtag gccactgcat atgtgtgcgc tttgcagccc atttcttcaa
     7801 aaatgagtcg ggcttcgtgc aggctgctgg cagtggcgcc gctgaggtag cgtttcacca
     7861 tcggaaagac gctccacatg gcgaatccct tgagtgccag gatgatgaaa acgccggctt
     7921 ccgactgtac gcgctgaatc agttccagat tttcgcgcag tttgcgttcc tccagcacat
     7981 aagccgccga gcggagagat gcgtattcgt tgttnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aaagcctcct cctcaagccg
     8161 gagcgtataa aaaccgcgct tttcctgagc gcccagcagc acgcttttgc cgtctttttt
     8221 cgacatgcgc caatgctccg gggccaattc cacgcagtaa ttgcagccga tgcacttttt
     8281 gcggttttga gaaacgatga tcatgcttcg gcgtccacta ttttgtacag cttatcggaa
     8341 gggcgaattt tggccggcac tttcagcgag caggacactc cctgccgggc gctgtccacc
     8401 gtttggtcgt ccactctgat ttcatctgct tcaaactcca aaacgccggt agccggaccg
     8461 gtcacgagca gcgaatcgcc ttttttcagg gtatggcttt ctattttgaa ctcgcccacc
     8521 tgtacttttg gaaaataatg cacgccgcgt ccgaggtaga tttttttggt ggtggctttg
     8581 gatccgtagc tgtcggccca ctcacccaac tgcttaccga ggtaataacc gccccaaaag
     8641 ccccggttat atactttttc cacctcctgc atccaggcat ctactttttc cgccgagaaa
     8701 tcgccgtttt gcaccgcgtt gatggcttcc cggtaggcgc gggtcacggc cttgacatat
     8761 tccggggcgc gggcgcgacc ttcaattttg agcacatcca cgccagtgtc gagcaactnn
     8821 nnnnnnnnga tccaaaaacg gcagcgtatt gaggtctttg ggcgacataa tgtactcgtt
     8881 ttcaatatga aaagcgtgtc cttcgtcgcg gtcggtcacc tcgtactgcc ggcggcaatt
     8941 ctgaatgcac accccccggt tggccgagga attgtgcgtg tgcagactca ggtagcactt
     9001 gccggaaatg gccatgcaca gcgcgccgtg ggcaaacacc tctatgcgca ccaactgccc
     9061 cgatggaccg gtgatctgat cgcgctgaat agcctccgtg atttcgcgca tttgcaccat
     9121 gctcagttca cgcgccagca ccatgacatc ggcaaaatgg gcgtaaaatt tcagagtttc
     9181 gatgttggaa atattgacct gagtggaaat atgtaccggc aaacctttag atgccgcgta
     9241 gccgatgacc gcctgatcgg aggcgataac ggcggagata ttgtgcttaa ccgcctcgtc
     9301 cacaatgcgt ttcatcnnnn nnnnnnnnnn nnnnnnnnnn nnngatgtac gatttcaccc
     9361 catgctcctt gcacaatttc gataccgcct ccagatcgtc taaagtgaaa ttcatggtag
     9421 cgcgggcgcg catgttcaac tgctcgatgc cgaaataaac cgaatctgct ccgccttgta
     9481 tggcggccat catagactcg aaattgcccg ccggggccat caactctata tgcttcttga
     9541 ccatttactg ttttgtaatt gcgccgcaaa ggtatgaaaa tctctacctt tgtccctctc
     9601 atccaagcta ctgaatatgt ctgcccaacg tacactcgaa atctgcgtag aatctgttac
     9661 ctctgccctc aatgctcaaa ctggcggcgc cgaccgcatc gaactctgcg acaacctgca
     9721 tcagggcggc atcacgcccg gcgcgggcaa aattgtatta gccaaaaagc tgctgcacat
     9781 tcctgtgttt gtgctcatcc gaccgcgcaa aggcgacttt ttgtattccg atatcgaatt
     9841 cgataccctg ctggaagaca tcgcgctggc ccggc
//
LOCUS       DAORLF010000137         9866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3686_length_9866_cov_1.863622, whole genome shotgun sequence.
ACCESSION   DAORLF010000137 DAORLF010000000
VERSION     DAORLF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_9866_cov_1.863622"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    264..451
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            624..860
                     /locus_tag="PK198_12155"
     CDS             624..860
                     /locus_tag="PK198_12155"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRF39531.1"
                     /translation="MKNKNVAGVLAILTGIFGTHRFYLGQRFLGALYFFLFWAGLAVT
                     IEEGNPIIMLLPALISLVDSIVFFAMPWEDFDRR"
     assembly_gap    868..892
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <893..1422
                     /locus_tag="PK198_12160"
     CDS             <893..1422
                     /locus_tag="PK198_12160"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRF39532.1"
                     /translation="PPRPDRGGRAPPPPPAPHVSKFDALKKEGIRKFRATQYAAAIAD
                     FLEALRIRENDPAIAFNLACCYSMLGQHVPGMYYLEMAVNNGFDDEQKILTHQALLNL
                     RLQPEFEAFARRFQAPPAQAAPPTSPAEEFLDLNIQESVEIPEPLLKLEDLKNRGILT
                     EEEFELQKQKMFGFR"
     gene            1551..>2640
                     /locus_tag="PK198_12165"
     CDS             1551..>2640
                     /locus_tag="PK198_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF39533.1"
                     /translation="MLSSSRLKTNLPLFQTADGLPYIHRDISWLSFNYRVLQEAMDTN
                     VPLFERIKFLAIYSSNLDEFFRVRMANQRNLLRVGKKTKRELETPPKMVVREIQHIVN
                     KQQQEFSRILEKQIVPELRKHGIYLLRRLDLDERQKEFVEDYFKDHMLPFVQPVLLVK
                     NKIRPFLINRALYLAVLLRSKDEPAAPEEYAIVNIPSDHLPRFVRLPSESGRYNVIIL
                     DDIVRHSLSWMFPGYDILDTFSIKLTRDAELYIDDEYSGDLIQKIKDSLAKRHVGPAS
                     RFVYDRDMPSPLLELLQNVLELEKYDILQEGRYHNNFDFFRFPDFGMNQLKYSALEPL
                     PHPVLTPAKDMFRVIRQKDKLLHPPFQAY"
     assembly_gap    2641..2678
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2679..3689
                     /locus_tag="PK198_12170"
     CDS             <2679..3689
                     /locus_tag="PK198_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRF39534.1"
                     /translation="EAARDPKVTHIKITQYRVARKSRILEALINAVKAGKQVSVFIEV
                     KARFDEEANIEWGERLEKAGVTVHYSFPGLKVHAKLALVRRMEPKGPRLYSYLATGNF
                     HEETAKVYSDYGLFTADERLTGEVARVFSFLETGKEPLQPFEHLLVGQFNLRSRLIEL
                     IELETAQAKVGKKARIVLKMNSLQDREMIEKLYQASQAGVEVKLIIRGICCLAAGKPG
                     FSENIEAISIVDRFLEHGRIFLFHHGGKELLYLSSADWMVRNLSFRVETSFPVYDPVI
                     KKEVMDYLNIQLGDNVKARTLDAEMSNADRRSNGDIPIRSQMETYYYLKRKIERLSDE
                     DS"
     gene            complement(3670..>3926)
                     /locus_tag="PK198_12175"
     CDS             complement(3670..>3926)
                     /locus_tag="PK198_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF39535.1"
                     /translation="LAGGFEHASRLMQSLQLVSLSSNLGDTRSIITHPHSTTHSKLSA
                     QDKAALGITEGLLRISVGLEHIEDLQEDFRKALEISYPRR"
     assembly_gap    3927..3969
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3970..4418)
                     /locus_tag="PK198_12180"
     CDS             complement(<3970..4418)
                     /locus_tag="PK198_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF39536.1"
                     /translation="MIYLETPSNPGLELVDMEMLGRISAEHGLLFYVDNCFATPILQN
                     PADYGAQLVAHSATKFIDGQGRVLGGVLTGTAEVMDRIRFFARHTGPSLSPFNAWVLS
                     KSLETLDVRMERHCSNALALARTLTECEGVKKVNYPWLETHPQFDLA"
     assembly_gap    4435..4600
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4601..4857)
                     /locus_tag="PK198_12185"
     CDS             complement(<4601..4857)
                     /locus_tag="PK198_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF39537.1"
                     /translation="MHKSTKAIRSQTPRSPFREHSTPLFLTSSFVFESAEQGRALFDE
                     TEEGNIYSRFSNPTVQEFTDKMAMLENHDEGLAFATGMAAV"
     assembly_gap    4883..4959
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4974..>5192
                     /locus_tag="PK198_12190"
     CDS             4974..>5192
                     /locus_tag="PK198_12190"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF39538.1"
                     /translation="MKKNLLLLWAVLLTFGAPLRASDGMWLPLLLKQLNEAEMKGLGM
                     KMSAEDIYSVNKSSLKDAIVHFGGGCTGE"
     assembly_gap    5193..5276
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5277..7145
                     /locus_tag="PK198_12195"
     CDS             <5277..7145
                     /locus_tag="PK198_12195"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF39539.1"
                     /translation="GYWAKSMSEEMPSKGLFVTFIIRIEDVTKAVLENVSAEMPEPMR
                     QLQVERNIAAVREAAARESWQETQVKPFFHGNQYYLFVTETYRDIRFVGAPPASIGSF
                     GKDTDNWVWPRHTGDFSLFRIYADKNNRPAEYSADNVPFKPRHFLPISLDGVQEGDFT
                     MIFGFPGATNAYLPAIAVQQTIEIMNPARIDVRNKTLGVYDAAMRADAQVRLQYVSKQ
                     ARLANSWKKWIGEKQGLEAADAVNKKLAFEAEFQRRVMAKEEWKWKYGTLLDDLNAKY
                     KDLRVYLQSLEYGLEIGSRNIELFQLANSLQSYVNILDNNGAQELAKRGPALVSYLEG
                     FYKDYRPEIDQKVFAELMALYIGKVKADFMANTAVEQATSYGKNYDQWASELFSESIL
                     TKPGTALQMARVKPDDLINAVKNDPAYLLARAIVAANNDKLSKPVSSIRLDIDRLQRQ
                     YMEAMMLAFPEKRFFPDANSTLRIGYGQVRGSQPKDGILYQPITYLDGVVEKYKPGDY
                     EYDVPAKLIQLHKNKDFGPYAAANGKMPVAFTGNNHTTGGNSGSPAIDAHGNLIGLNF
                     DRQWEGTMSDIFYDPEICRNIMVDIRYVLFLIDKFGGAGHLVKEMKLVRPKTGSRK"
     gene            7199..7324
                     /locus_tag="PK198_12200"
     CDS             7199..7324
                     /locus_tag="PK198_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39540.1"
                     /translation="MSTQDIRWQQRFQNFLKAFSLLEEAVTKYQKEGISDWITAV"
     gene            7324..7518
                     /locus_tag="PK198_12205"
     CDS             7324..7518
                     /locus_tag="PK198_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39541.1"
                     /translation="MKLENQLDDLLLPYKIDLSLYRNISNPDLTDHIQRVGKVFYEKT
                     PHPDSPKKSARPKHGKTNTK"
     gene            7515..8390
                     /gene="tesB"
                     /locus_tag="PK198_12210"
     CDS             7515..8390
                     /gene="tesB"
                     /locus_tag="PK198_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HRF39542.1"
                     /translation="MKTINELLDLLQLEAIEENLFRGHSTNIGSGRVFGGQVLAQSLW
                     AAMQTAPPGRVLHSLHSYFLLAGDISQPIIFEVDRIRDGGSFTTRRVKAIQKGKAIFL
                     MSASFHAPEPGYDHHLNMPNVSPPEMLVSWDDLVSQYGKMLPDNIRRFLEIERPIEFK
                     PVEVVSLDLKGAAQPVRHVWMRSKGEMPQDLALHQTVLAYASDYNLLTTALLPHGNEA
                     SFGDYMLASLDHAMWFHRDFKMDDWLLYAIDSPSASGARGFTRGSVFTRDGALVASVV
                     QEGLMRRVTKTGGNS"
     gene            complement(8368..>9866)
                     /locus_tag="PK198_12215"
     CDS             complement(8368..>9866)
                     /locus_tag="PK198_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39543.1"
                     /translation="YALSPKNRLYLSFYSGHDAFENNGFQARTFTLRSNIPGETFLIR
                     YDRSYKDSYHWGNTIGSLRWNHVAGNKLFVNTTATFSRLDVNIGYENADSLLLLDPLI
                     LLGRNINVGRYQSGIEERGLRVDADYVPVPGQYWRFGVNWAHRKFRPGALVYDERTES
                     ENPGLANDPAEATELALYAENEFSIGKSLTLNLGAHLASWNTGKKNHSALQPRLSVQW
                     QFSPQWKWRAAHSQMAQFLHFLTNSDIGLPTDLWVPSTDQIAPERAGQTETGFEWPGK
                     GWLLEWDVYYKKMRNLLAFAEGASFLNDWEQNVTVGEGAAYGMETMIQYRTERTTAWV
                     AYALGKTERRFDRINLGRTFPFKYDRRHDLKIVLSHQLRPWLSLSGNWLLSSGFAYSL
                     PVMEFSFRVPGQTTAPVIVQDFGSKNRFRMPAYHRLDLSAKFSFRTSGLEHDINLGVY
                     NAYNRRNPLYYNLRTDIENVDNELRAKASFVQVWLLPVMPSFSYSLRF"
BASE COUNT         2240 a         2541 c         2436 g         2028 t
ORIGIN      
        1 ttttttggca gaatcggcct ggcagttgaa aaccgtacag gagaattttc cggagatgga
       61 gtttcatttt accagtgagt attgaggttt cggggaggaa cgggcggatt tcggattttc
      121 tgacaggaac aggttgattt cggatttcgg atttccgatt cctgattcct gatttcggat
      181 tggcaaaccg cctaccgaaa accagtttcg agtttcaggt ttcgggggta gtgaaggggt
      241 gactcagcaa gtttcagttc cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgtggga aggcgggttc aaggtttcgg
      481 gttgcctcac gcttaccgcg tcacggcccg cgcaaggcgc ggctaccgaa aaaccattcg
      541 tccataaaaa cagcgcgaaa gtatatggat tttggatact gaggcaaaag caaccttttt
      601 gcgctttttt tattcattaa ggtatgaaga acaagaacgt agccggtgta ttggccatcc
      661 tgaccggcat tttcggcaca caccgctttt atctggggca gcgttttctg ggcgcgcttt
      721 acttttttct cttctgggcc ggcttagccg ttaccattga agaaggtaat cccattatca
      781 tgttgctgcc ggcgctcatc agtttggtgg acagcatcgt gttttttgcc atgccctggg
      841 aggatttcga ccgcaggtaa aacaaggnnn nnnnnnnnnn nnnnnnnnnn nnggccaccg
      901 cgtcctgata gagggggacg ggcaccgccc ccgccgcctg cgccgcatgt ttccaaattc
      961 gatgcgctca aaaaagaggg catccgtaaa ttccgggcca cccaatacgc cgccgccatt
     1021 gccgacttcc tcgaagcgct gagaatcaga gagaacgacc ctgccattgc cttcaacctc
     1081 gcctgctgtt acagcatgtt gggccagcat gtgccgggga tgtactacct ggaaatggca
     1141 gtcaacaacg gctttgacga cgagcaaaaa atcctgacac accaagccct gctcaatctt
     1201 cgccttcagc cggagtttga ggcatttgcc cggcgttttc aggcgccgcc cgcacaggcc
     1261 gcgccgccaa catctcctgc cgaggagttt ttagacctca atattcagga atcggtagaa
     1321 atacccgaac ccctgctcaa actggaagac ctcaaaaacc ggggcatcct caccgaagaa
     1381 gaatttgaat tgcaaaagca gaagatgttt gggtttcggt aggtttttgg aagggggctg
     1441 tgggcggcat caaaaaataa cattcggcaa tcctaacact ttctaacttc gccctttgca
     1501 aacaatacgc cggcgcgttt gtcaattgat aaacaaaaca aaaagcaacg atgctgtctt
     1561 cttcacggct gaaaaccaat ttgcctctct tccaaactgc tgacggcctg ccttatattc
     1621 atcgggatat cagttggtta tcattcaatt accgcgtcct tcaggaggcg atggatacca
     1681 atgtgccgct gtttgaacgc atcaagtttc tggccattta ctcttccaac ctcgacgaat
     1741 ttttccgcgt gcgcatggcc aatcagcgca acctcctccg cgtaggcaaa aaaaccaaac
     1801 gcgaattgga aactccgccc aaaatggtgg tgcgcgaaat tcagcacatc gtcaataagc
     1861 aacagcagga attcagccgt attttggaga aacaaattgt gcccgaactg cgcaagcacg
     1921 gcatctacct gttgcgccgc cttgatctgg acgagcgcca gaaagaattt gtagaggact
     1981 attttaaaga tcacatgttg ccctttgtgc agcctgtttt attggtcaaa aacaaaatcc
     2041 ggccttttct catcaaccgc gctttgtacc tcgccgtgct cttgcgcagc aaagacgaac
     2101 ccgccgcgcc cgaagagtat gccatcgtca atattccttc cgatcacctg cctcgttttg
     2161 tgcgattgcc ctcggaatca ggtcgttaca atgtcattat cctcgacgac atcgtgcggc
     2221 acagcctctc ctggatgttt ccgggctacg atattctcga cactttttcc atcaaactca
     2281 cccgcgacgc cgagctgtac attgacgatg agtactccgg cgacctcatc caaaagatca
     2341 aagacagcct cgccaaacgc catgtggggc cggcttcccg ctttgtttat gaccgggaca
     2401 tgccttcgcc tttgttggaa ttgctccaaa acgtgctgga attagaaaaa tacgacatcc
     2461 tgcaagaggg ccgctaccac aacaatttcg attttttccg cttcccggat tttggcatga
     2521 accaactgaa atacagcgcg ttggagccgc tgcctcaccc cgttctcacc ccggcaaaag
     2581 atatgttccg cgtcattcgg cagaaagaca agcttttgca tccgccgttt caggcttatg
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agcggcccgc gatcctaaag
     2701 tcacgcacat caaaataacg cagtaccggg tggcccgcaa atcgcgcatc ctagaggcgc
     2761 tcatcaacgc cgtcaaggcg ggcaaacagg tgtcggtatt catagaggta aaagcccgtt
     2821 tcgacgaaga ggccaatatc gaatggggtg agcgcttgga aaaagccggc gtgacggtgc
     2881 attacagttt tcccggcctg aaagtacacg ccaaattggc cttagtgcgt cgtatggaac
     2941 ccaaagggcc gcgcctgtac agctatttgg ccaccggcaa ttttcatgaa gaaaccgcca
     3001 aagtctattc cgattacggc ctctttacgg ctgatgaacg gctcaccgga gaagtggcgc
     3061 gcgtgttttc ttttttggaa accggcaaag aaccgctgca accatttgaa catttgttgg
     3121 tggggcagtt caatctgagg agcaggctga tcgagttgat tgagctggaa acagcacagg
     3181 ccaaagtcgg taaaaaggcg cgtattgttc tgaaaatgaa cagcctgcaa gaccgggaaa
     3241 tgatcgaaaa actgtatcag gcttcgcagg cgggcgtgga ggtaaaactc atcatacgcg
     3301 gcatctgctg tctggcggca ggcaaacccg gtttcagcga aaacatcgag gccatcagca
     3361 ttgtggatcg ctttttggaa cacgggcgca tttttctgtt tcaccacggc ggcaaagaac
     3421 tgctttacct gtcgagtgcc gactggatgg tgcgcaacct ctctttccgc gtcgaaacct
     3481 cctttccggt ttacgacccg gtcatcaaaa aagaggtcat ggattatttg aatatccaac
     3541 tgggcgacaa tgtaaaagca cgcactctgg atgccgaaat gagcaacgct gaccgccgca
     3601 gcaatggaga cattcccatc cgctcgcaaa tggagaccta ttactacctc aaacgcaaaa
     3661 ttgagcgact cagcgacgag gatagctgat ttccaaagcc ttacgaaaat cttcctgcaa
     3721 atcttcaata tgctccaagc ccaccgaaat acgcagcagc ccctcggtga tgcccaatgc
     3781 agccttatct tgtgccgata atttggaatg agtggtgctg tgtggatgcg tgatgatgct
     3841 gcgcgtatcg cccagattgg aactcagcga taccaactgc aaggactgca tcaggcggct
     3901 cgcgtgctca aatccgccgg ccagttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnt tggccaaatc aaactgcgga tgcgtttcca accaggggta atttaccttt
     4021 ttcacgccct cgcattcggt cagggtgcgg gccaatgcca gcgcgttgga gcagtgccgt
     4081 tccatgcgga catccaatgt ttccaagctt ttcgacaata cccaggcatt gaacggcgac
     4141 agcgaaggcc ccgtgtgccg cgcaaaaaaa cggatgcgat ccatgacctc ggcagtaccg
     4201 gtgagcactc cgcccagcac gcgcccctgg ccgtctataa atttggtggc cgagtgcgcc
     4261 acgagttgcg cgccgtagtc ggccggattt tgcaaaatcg gggtggcgaa gcagttgtcc
     4321 acataaaaaa ggaggccatg ttcggcactg atgcgaccga gcatttccat gtccaccaat
     4381 tccaggcccg gattggaggg cgtttccaga tagatcattt ttgtttcggg gcgcnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaccgccg ccatgcccgt
     4621 agcaaaagcc aggccctcgt cgtggttttc cagcatggcc attttgtcgg taaactcctg
     4681 caccgtcgga ttggaaaaac ggctgtaaat attgccttcc tccgtctcgt caaatagtgc
     4741 gcggccctgc tctgcgcttt caaaaacaaa actggacgtg agaaacagcg gagtgctgtg
     4801 ctcgcggaaa gggctgcggg gcgtctggct gcggatggct ttggtggact tgtgcatgtg
     4861 ccggctgttg tttggtgcgg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aacatcatcc gacatgaaaa
     4981 agaacctcct gctcttgtgg gccgtccttc tcacttttgg cgccccattg cgcgcaagcg
     5041 acggtatgtg gctgccgctg ctgctcaaac aactcaacga ggccgaaatg aagggcttgg
     5101 gcatgaaaat gagtgccgaa gacatttaca gcgtcaacaa aagcagcctc aaagatgcta
     5161 ttgtacattt cgggggcggc tgcaccggcg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggct
     5281 actgggccaa aagcatgagc gaagaaatgc ccagcaaggg tctgttcgtc acctttatca
     5341 ttcgtataga ggacgttacg aaagccgtgc tggaaaatgt gagcgccgaa atgcccgaac
     5401 ccatgcggca attgcaggta gagcgcaaca tagccgccgt gcgcgaagcc gccgcccgcg
     5461 aaagctggca ggaaacgcag gtgaagccct tttttcatgg caaccaatac tacctctttg
     5521 tcaccgaaac ctaccgcgat attcgcttcg taggcgcgcc gccggcctcc atcggcagct
     5581 tcggcaaaga caccgacaac tgggtgtggc cccggcatac gggcgacttc tcgctcttcc
     5641 gcatctatgc cgacaaaaac aaccgccccg ccgagtactc cgccgacaat gtgccgttca
     5701 agccgcgcca cttcctgccc atctccttag acggcgtgca ggaaggcgat ttcaccatga
     5761 tctttggttt tccgggcgct accaatgcct acctgccggc cattgcagtg cagcaaacca
     5821 tcgaaatcat gaaccccgcc cgcatagatg tgcgcaacaa aaccctcggc gtgtatgatg
     5881 ccgccatgcg cgccgatgcg caggtgaggc tgcaatatgt atcgaaacaa gcccgcttag
     5941 ccaattcctg gaaaaaatgg atcggtgaaa aacagggcct cgaagccgcc gacgccgtga
     6001 ataaaaaatt ggcttttgaa gcggaatttc agcgccgcgt gatggccaaa gaagaatgga
     6061 aatggaaata cggcacgctc ttagacgatc tcaatgccaa atacaaagac ctgcgcgttt
     6121 atctccagag tttggaatac ggcctcgaaa taggtagtcg caacatcgaa ctgtttcagt
     6181 tggccaacag cctgcaatct tacgtcaata ttttagacaa caacggcgcg caggaattgg
     6241 ccaaacgcgg ccccgccttg gtgagctacc tcgaaggttt ttacaaagat taccgcccgg
     6301 aaattgacca gaaagtcttt gcagaactca tggcgctgta catcggcaag gtaaaggcag
     6361 actttatggc caacaccgcc gtagaacagg cgacttccta cggcaaaaat tacgatcaat
     6421 gggcctccga actcttctct gaaagcatcc tcaccaagcc cggcacagcc ctgcaaatgg
     6481 cccgtgtgaa accggacgac ctcatcaacg ccgtcaaaaa cgacccggcc taccttctgg
     6541 cccgcgccat tgtagccgcc aacaacgaca aactctccaa gcccgttagc tccattcgcc
     6601 tcgacattga ccgcctccag cgccaataca tggaggccat gatgctcgct ttccccgaaa
     6661 agcgcttctt ccccgacgcc aacagcaccc tccgcattgg ctacgggcag gtgcgcggtt
     6721 cgcagcccaa ggatggtatt ttgtaccaac ccatcactta tctggacggc gtggtagaaa
     6781 agtacaagcc cggcgactac gagtatgatg tgcccgccaa actcatacaa ctgcacaaaa
     6841 acaaggactt cggcccttat gcagccgcca acggcaaaat gcccgtcgcc ttcaccggca
     6901 acaaccacac caccggcggc aattcgggca gcccggccat agacgcgcac ggcaacctca
     6961 tcggcctcaa tttcgaccgc caatgggagg gcaccatgag cgatattttt tacgacccgg
     7021 aaatctgtcg caacatcatg gtggacattc gctatgtgtt gttcctgatt gacaaattcg
     7081 gcggcgccgg ccacttggtc aaagagatga aattggtgcg ccccaagacg gggtcgcgta
     7141 aatagggaga agggggaggc gtcagctttg ggttgccgcc tctaaaatca gcacgcttat
     7201 gagtacacaa gacatcagat ggcagcagcg gtttcagaat tttctaaaag ccttttcgct
     7261 tttggaagaa gcggtaacga aatatcaaaa ggaaggtatt tccgattgga ttacagcagt
     7321 ttgatgaaat tggaaaatca attggatgac ttgttattgc cttacaaaat agacctgtcg
     7381 ctttatcgca acatcagcaa tcccgacctg acagaccata ttcaacgagt gggaaaggtg
     7441 ttttatgaaa aaacgccaca tcccgattca cccaaaaaat ccgccaggcc caagcacggc
     7501 aaaaccaaca caaaatgaag accatcaacg aattactcga cctcctgcaa ttggaggcca
     7561 tcgaagaaaa cctgtttcgc ggccacagca ccaacatcgg cagcgggcgc gtattcggcg
     7621 ggcaggtact cgcacaatcc ctttgggcgg ccatgcaaac cgcgcctccc ggcagggtgc
     7681 tgcactcgct ccattcttat tttctgttgg ccggcgacat ttcccaaccc atcatttttg
     7741 aggtggaccg catccgcgac ggcggcagct tcaccacccg ccgcgtgaaa gccatccaga
     7801 aaggcaaggc tattttcctc atgtcggcct cttttcatgc gcccgaaccc ggctacgacc
     7861 accacctcaa catgcccaat gtgtcgccgc cggagatgtt ggtgagttgg gacgacttgg
     7921 tgagccaata cgggaaaatg ctgcccgaca acatccgccg gtttttggaa attgagcgcc
     7981 ccatcgagtt caaacctgtt gaggtggtca gcttagacct caaaggcgcg gcccagccgg
     8041 tgcgccacgt ttggatgcgc tccaaaggcg aaatgccgca agacctggcc ctgcatcaga
     8101 cggtattggc ctacgccagc gattacaacc tgctcaccac cgctttgctg ccgcacggca
     8161 acgaggcttc tttcggcgat tacatgctgg ccagtttgga ccatgccatg tggtttcacc
     8221 gcgatttcaa aatggatgac tggctgctct acgccataga cagtcccagt gcatcggggg
     8281 cgcgcggatt cacaagaggc agcgttttta cacgcgacgg cgcgctggta gcttcggtag
     8341 tgcaggaggg cctgatgcgg cgcgtgacta aaaccggagg gaatagctaa aggagggcat
     8401 gaccggcaac aaccatacct gcacaaagga ggctttggcg cgcagttcgt tgtccacgtt
     8461 ttcaatgtcg gtacggaggt tgtaatacaa ggggttgcgg cgattgtagg cgttgtacac
     8521 ccccaggttg atgtcatgtt cgaggcccga tgtgcggaag gagaatttgg ccgacaggtc
     8581 taaacgatgg taagccggca tgcggaagcg gtttttggaa ccaaaatcct gcacaatgac
     8641 cggcgcggtc gtctggcccg gcaccctgaa actgaactcc atcacgggca ggctgtatgc
     8701 aaaacctgaa ctcagcagcc aattgccgga cagcgacaac cagggccgca actgatgcga
     8761 caatacgatt ttgaggtcgt gccggcggtc gtatttgaac ggaaaagtcc tgccgaggtt
     8821 gatgcgatca aagcggcgtt cggttttgcc cagcgcgtaa gccacccagg ccgtggtgcg
     8881 ctccgtccgg tattgtatca tggtttccat gccgtaggcg gcaccctcgc cgacagtcac
     8941 gttttgctcc cagtcgttga gaaaagaggc cccttcggcg aaagccagca ggttgcgcat
     9001 ttttttgtaa tacacgtccc attccaacag ccagcctttt ccgggccatt caaagccggt
     9061 ttctgtttgc ccggcgcgtt cgggcgcaat ctgatcggtg gagggcaccc ataaatcggt
     9121 gggcaggcca atgtcggagt tggtcagaaa atgcaaaaac tgggccatct gggaatgtgc
     9181 cgcccgccat ttccactgag gcgaaaactg ccattgaacg gagaggcgcg gctgtagggc
     9241 gctgtgattt tttttgcctg tattccagga agccaaatgt gctccgaggt tgagtgtcaa
     9301 ggacttgccg atgctgaatt cattttcggc atacagggcc aattcagtgg cctcggcagg
     9361 gtcattggcc aatccgggat tttcactctc ggtgcgctcg tcatacacca aagcgccggg
     9421 cctgaatttc cggtgcgccc agttgactcc gaaacgccag tattggccgg gcacgggcac
     9481 atagtcggca tccacgcgca ggccgcgttc ttcaatgccc gattggtagc gccccacatt
     9541 gatgttgcgc cccagcagga tgagcggatc gagcaggagc agggagtcgg cgttttcata
     9601 accgatgttc acatccaatc ggctgaaagt ggcggtggta ttcacaaaga gtttattccc
     9661 ggcaacgtgg ttccagcgaa gcgagccgat ggtattgccc cagtggtagg agtctttgta
     9721 tgaacggtcg taacggatca ggaaagtttc gccaggaatg ttgctgcgca gcgtaaatgt
     9781 acgggcctga aacccattat tttcaaaggc atcgtggccc gaatagaagc tgaggtagag
     9841 ccggtttttg ggagaaagag cgtagt
//
LOCUS       DAORLF010000138         9864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3690_length_9864_cov_1.987257, whole genome shotgun sequence.
ACCESSION   DAORLF010000138 DAORLF010000000
VERSION     DAORLF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_9864_cov_1.987257"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>255)
                     /locus_tag="PK198_12220"
     CDS             complement(<1..>255)
                     /locus_tag="PK198_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39544.1"
                     /translation="NWVRTHATQGQLAGVSGLQDWNVWLKFKALQAGNWSLYAIGGAS
                     GPASNYLPDYVPLNLGLGSYEGSLRLLTEYDFGQGFFGRAF"
     assembly_gap    256..270
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<271..469)
                     /locus_tag="PK198_12225"
     CDS             complement(<271..469)
                     /locus_tag="PK198_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRF39545.1"
                     /translation="MKAQTPIDGIMMSKNSVCTALTFTQDNWDHYWEGTLKRDNGNIG
                     VAQRQTIAPMFSWGITDRINLL"
     assembly_gap    587..596
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    944..971
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<972..1295)
                     /locus_tag="PK198_12230"
     CDS             complement(<972..1295)
                     /locus_tag="PK198_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39546.1"
                     /translation="MRIFYSISLAALTLVSWMEGLHLCRQYIREVENQMEAISFVEQV
                     LAQRYASVLQQGSTVKEVDSSFSGRQGYIYPGDFVLQSEIEGDTAHFIIESSTDSQTD
                     YTFVQS"
     assembly_gap    1485..1502
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1503..2285)
                     /locus_tag="PK198_12235"
     CDS             complement(<1503..2285)
                     /locus_tag="PK198_12235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF39547.1"
                     /translation="MVPDSPDADIQPEQPQPIRRLPESWPPPLMVLALTLIAIIMAVI
                     LGGVITHVLGIVFHYDLVDIMNTIETENTQRHRDILRGSNLVSHLLAFTGASVAVMFF
                     VYRKEWATRLGLSRFPGWPAIAAAAGFMLFALPWVQFTYWLNKQIPLPAWLADMENQT
                     TGIIQAILVMESPWELAFSLLVIALAPAVGEELLFRGIVQPQISRLWNKPVAAVWVTA
                     ILFSIIHFQFAGFIPRMLLGAGLGYLLLWTRSLWAPIAGHFVI"
     gene            2536..3987
                     /locus_tag="PK198_12240"
     CDS             2536..3987
                     /locus_tag="PK198_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRF39548.1"
                     /translation="MEPGSLQKFLTVLFLSLFLPSLSFGSVYNGSIELELEYDENSVA
                     ERMLAMPDMTIRPRRDPVAMGYIRNYLTRSRRTAELIIGRSILYFPMFDEQLKRHNLP
                     KELKYLPVVESALNPKAVSPAGAGGLWQFMPGTGRLEGLQANKYIDERFDPIKSTEAA
                     MKHLTRLYEMFGDWELVLAAYNSGSGNVTRAIKRGRSQDFWRIQRYLPRETRNYVPAF
                     MAATYLCEHFMLHGIEPAAPEMDMQLTESIKVYNGFSFFELATLTGLSVETLESLNPA
                     YVGGYIPQNANGYYLTLPRRVMPAVLEFLAAKLPDDSDPEPIFVGGPVALYNTPGAES
                     YYNEHVYTVEEDDEFKSIDALADKLRCTPQHLRIWNRLNTPTLVPGQEWRYFGPKAYK
                     RMTLNKMPIVEAPIALPFRQVGTLEYTTSIEAAGIEPDNAERFLYISISGAERLPEIA
                     RRYEGVSVEDLRRLNRVMGNPVFRDRVVKIKEL"
     gene            4136..5041
                     /locus_tag="PK198_12245"
     CDS             4136..5041
                     /locus_tag="PK198_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRF39549.1"
                     /translation="MALISPGSYAPDDAVQKAYLNLEGLGLKVKPGKYLRAQRGFTAG
                     SDQQRLDDLHAAFSDTEASAVWCVRGGYGCTRLLPFIDYPLIRQNPKILIGYSDITAL
                     LQAIHIETGLVGFHGPVASATFTDYAKGQLVKVLMEPAAMHTIPVAESQKEKKDSEYQ
                     PVTINEGKAEGILSGGNLSLLAAMAGTPHAFDFTNKLVFIEEVGERPYRLDRMLTTLL
                     QASNLRKAAGVALGVFTDCQPKKDELSLSMMDTLRDRLEGLGIPVVYGLSFGHISDNC
                     TLPVGISASLDVQQRSITILETPVL"
     gene            complement(5044..6963)
                     /locus_tag="PK198_12250"
     CDS             complement(5044..6963)
                     /locus_tag="PK198_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF39550.1"
                     /translation="MQQRILVIEDNPADAAIIHIYFEETAFGHTIFQADSLKRGFGIM
                     REHSIDLVLLDLTLTDSIGFSTLEKFLTEFPNVPVIVMTGLNNEILGVQCVRAGAQDF
                     LVKGEFDSKALVKSIRHAQQRFQTQTKLQESAQKLSHQQKRLQEAQELANFANWEMDV
                     VTNAMKWGEEMYRIFGLPQTEAALSLGDYLQYVFFDDKEAVETFFTTALQAEEIQKIE
                     HRIVVGTRVKHLSLRAKVQLDQDSGQVLLIGSAQDVSEQMTPILSSGSSKGVSELWRK
                     EILAELKEQIQKPLFEALDLAHLLQKTATPSQQELLPEFKSGLNEISLAVNTLLMAQT
                     DIRNASAQEAAPFVFNSFFTHFQEVTEAQAAKSGCKVHLSLPADFPTTLIGNEHLLHT
                     LLCSFFKCALAPKGRPGTIVIQVNISKVSGDQHSMKIEYLPNDKQMPADMLKAVMDAA
                     DFSMVSTPDLRRKATTMAVSGQLLKALNGKYALRKDEKGLCESFELSIPVKIPETKAD
                     GGANLFLPVAPSILIVDDNRMHLLSIRNVVKNVMADATVELAGGGKAAVSIAGQHKFD
                     LILLDLQMPDMNGFDTLAALKKHNQAPVIATTSTPSPEEKDLCINSGFRGYVGKPIQP
                     DALSEEVKRIMQASK"
     gene            complement(7056..7478)
                     /locus_tag="PK198_12255"
     CDS             complement(7056..7478)
                     /locus_tag="PK198_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF39551.1"
                     /translation="MKHIHILLVEDNPGDIRLTQEAFHEAGVPATLDVAMDGQEAVAF
                     LEKNVRSLPDLILLDLNLPKWSGKEVLHRIKNHPEFKRIPVLILTTSKSEQDVLESYQ
                     LNANSYLLKPMDFDDFLEIVDRINSFWLQTAILPPQHN"
     gene            7588..8571
                     /locus_tag="PK198_12260"
     CDS             7588..8571
                     /locus_tag="PK198_12260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF39552.1"
                     /translation="MQKNQSQLEQFFTEYLENQVFDREPHTLYAPIDYIMALGGKRLR
                     PLLTLMGCQMFGRPAGDALPAALAVEVFHNFSLVHDDIMDEAPLRRGMPTVHQKYNAN
                     TAILSGDAMLIEAYRFLCQSPADASLPRLMHIFNHTALEVCEGQAADMDFEKRDDVSL
                     PEYLRMIEQKTAALIGGALALGAVIGGASEEDAAHLDAFGRNIGIAFQIQDDLLDTFG
                     DPEKFGKKTGGDIARNKKTFLLLKALESATPALRQELMALYSETSPALESAKIERVKA
                     VFSELQTAASAQAAKTDYQQRAFQHLAAIDAPEEGKTVVRDFANWLFSRDV"
     gene            complement(8742..>9042)
                     /locus_tag="PK198_12265"
     CDS             complement(8742..>9042)
                     /locus_tag="PK198_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF39553.1"
                     /translation="ILAEDAGQIVGIALYHFAYSTWKGRMMYLEDFVVTDSRRGQGIG
                     RILFDAFLEECRVQECRVVKWQVLDWNEPALNFYKKYDAAIETNWWNGKIYTMIK"
     assembly_gap    9043..9069
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9214..>9864)
                     /locus_tag="PK198_12270"
     CDS             complement(9214..>9864)
                     /locus_tag="PK198_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF39554.1"
                     /translation="LRKHPLPRQAHIVDMGAGKGYLTFALYDHLKNNLGMEPQVLGLE
                     IRPHLVEFANHLARQCGFEGLRFQTQDIRDFEGKSTDMLIALHACDTLTDVALAEGIR
                     AGAGIILAAPCCHKQIRQQIQPQGILKTMLRHGILEERQAELLTDSIRSLLLEAEGYQ
                     TKVFEFVSIEHTNKNLMIAATRAQPDADARAKVQEIKQMFGISSHFLEQLLEHRNL"
BASE COUNT         2404 a         2377 c         2516 g         2469 t
ORIGIN      
        1 gaaggcccgt ccaaaaaatc cttggccgaa atcgtactcc gtaagcaggc ggagggagcc
       61 ttcatagctt cccagaccca gattgagtgg aacgtaatcg ggcaggtagt tggaggccgg
      121 ccccgatgca ccgccgatgg cataaagcga ccaattgccg gcttgcagtg ctttgaattt
      181 gagccacaca ttccagtcct gcaaaccgct gacacccgcc aattgacctt gcgtggcgtg
      241 ggtgcgcacc cagttnnnnn nnnnnnnnnn ccagaaggtt gatgcggtcg gtgatgcccc
      301 aactaaacat aggcgcgatg gtttgccgtt gcgccacgcc gatgttgccg ttatcgcgct
      361 tgagggtacc ttcccagtaa tggtcccaat tatcctgtgt gaatgtcagc gcagtacaaa
      421 cactgttttt gctcatcata atgccgtcaa tgggcgtttg tgctttcaag ttgcacattg
      481 cgcaaatgaa taaaacaccg aagatcgttc ggctcaaaaa atcccttttc atggtgtaaa
      541 tggtctttaa aggagtgaat caaccgacga tctcattgct ccttttnnnn nnnnnnaaga
      601 ttgaaagctg cccggattgt gtgtgggcag agccggacta ctattgttgc cgataagttc
      661 atgcagcaaa cctgaaccag cggtctgatt tgacacacga agtgcctctt cggtggaaag
      721 aaagaaagaa tgagatcgaa aataaatcag aaaggaaaaa tcagcctatt tccggcggtt
      781 gaacggggca atcctcggct gacgaggcgg ggaaaaaacg gtagggcgga tagctttgag
      841 aaactaattc aaacgcggtt tttgaagtta tatgtgcatc ccaaatggct gtgtaccagg
      901 gaaaaaaatc ttccaatgcg gtatggggcg gatattcgtt ttgnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nagactgaac aaatgtgtaa tctgtctgag agtcggtaga ggattcaatg
     1021 ataaaatggg cggtatctcc ttcaatttcc gattgcaaca caaagtcgcc ggggtagatg
     1081 tagccctgcc gcccgctgaa agacgaatcc acttctttta cggtgctgcc ctgctgcaaa
     1141 acggaagcgt acctctgggc cagaacctgc tccacaaaac tgatcgcttc catctggttc
     1201 tctacttccc tgatatattg ccggcacaaa tgcagccctt ccatccagga aacgagcgtc
     1261 agtgcggcta atgatatgga gtagaaaatt cgcataaaaa cagatagtag gtgcaaaagt
     1321 aagatgactt acccggattc aggtaattta tttttttgaa ctaaatatct gcccagcaaa
     1381 aacatcaccg gcagtaccaa agcgcagggg atcagcaatt ggttgatgtt cagagacttt
     1441 tcagcctgtt ccaaatctgt tttggccaca tattgtgcta tgacnnnnnn nnnnnnnnnn
     1501 nngataacga aatgtcccgc tataggcgcc cacaggctgc gcgtccacag tagcaggtac
     1561 cccagaccgg cgccgagcaa catgcgcgga ataaatcctg caaactgaaa atgaatgatg
     1621 ctgaacaata tggccgtgac ccacaccgcc gcaacaggct tgttccaaag gcgcgaaatc
     1681 tgcggctgca caatgccccg aaacaacaac tcttcgccca ccgccggcgc cagtgcaatg
     1741 accaagagcg aaaaggccaa ttcccagggg ctttccatca ccaaaatggc ctgtatgata
     1801 ccagtggttt gattctccat atcggcgagc caggcgggca ggggaatttg tttgttgagc
     1861 cagtaggtaa attggaccca gggcagcgca aaaagcataa agccggcggc ggcggctata
     1921 gcaggccatc cggggaagcg actcaggccc aatcgggtgg cccattcctt tcggtaaaca
     1981 aaaaacataa cggccacaga cgctccggtg aacgccagca aatggctgac taaattggag
     2041 ccgcgcagaa tatcccggtg tcgctgtgtg ttttccgttt caatcgtgtt cataatatca
     2101 acgagatcgt aatggaaaac tatgcccaat acatgtgtga tgacgccgcc caaaatgacg
     2161 gccatgatga tggctatcag cgtcaaggcc agtaccatca ggggcggcgg ccagctttcg
     2221 ggcagcctgc gtataggttg cggctgttct ggttggatgt ctgcatcggg tgaatctggc
     2281 accataaaac gattgttttg ggggccgaaa gtagggaaaa gagaggcatc tacgcaggtt
     2341 ttatctcgac atttataaaa atgcgatttt tttagactga taatcagtta cttacaaata
     2401 atgtggaaaa ctttttccct tcaaattagt gcgttaaggc attccgtgtc tatgtttgca
     2461 atacggctcg gcattttttt gtcttcaaaa acagcataaa tcattgagcc acaattgtta
     2521 acaaaatcaa ttattatgga gccaggtagt ttacaaaaat tcctgaccgt tctttttctc
     2581 tccctttttc tcccctcctt gtcttttggg tctgtttaca acggtagcat tgagttggaa
     2641 ttggaatatg atgaaaactc ggtagcagaa cgcatgctcg ccatgcctga catgacgatc
     2701 cgcccgcgcc gcgatcctgt ggcaatgggg tacattcgaa attatctgac ccgcagtcgc
     2761 cgcacggccg aactcatcat aggccgcagc attttgtact tccccatgtt tgacgaacaa
     2821 ctcaagcgcc ataatctgcc caaagagttg aaatacctgc ccgtagtgga atcggcgctc
     2881 aatcccaaag ccgtttctcc ggcaggcgcg ggcggtttgt ggcaatttat gcccggcacc
     2941 ggcaggctgg aaggcttgca agccaacaaa tacattgacg aacgcttcga tcccatcaag
     3001 tccaccgaag cagctatgaa gcacctcacc cgcctgtatg aaatgttcgg cgattgggaa
     3061 ttggtgctgg ccgcctacaa cagcggctcc ggcaatgtca cccgcgccat caagcgcgga
     3121 cgcagccagg atttttggcg catacagagg tacctgcctc gcgaaacccg caattatgtg
     3181 ccggctttca tggcagccac ttatttgtgc gagcacttta tgctgcacgg catagagcct
     3241 gctgctcccg aaatggacat gcaactcacg gagagcatca aagtgtataa tgggttcagt
     3301 ttctttgagt tggctacgct cacaggtctt tcggttgaga ctctggagag cctcaacccg
     3361 gcttatgtgg gcggttatat tcctcaaaac gcgaatggtt actacctgac cctgccacgc
     3421 cgggtaatgc ctgccgtgtt ggagtttctg gcggccaagc tgcccgacga ttctgatccg
     3481 gagccgattt tcgtcggtgg ccctgtagca ctctacaata ctccgggcgc tgaatcttat
     3541 tacaacgaac acgtttatac ggtagaggaa gatgatgaat tcaaatccat agatgctttg
     3601 gctgataaac tccgttgcac gccgcaacac cttcgtatct ggaaccggct caatacgcca
     3661 acgctcgttc ccggtcagga gtggagatat tttggcccta aagcctacaa acgcatgacc
     3721 ctgaataaaa tgcccatcgt ggaggcgccg attgccttgc ctttccggca ggtaggaacg
     3781 ctggaataca ccacatccat agaggctgcc ggtattgaac ccgacaatgc ggagcgcttc
     3841 ctgtatattt ctatttcagg agccgagcgc ctgcccgaaa ttgcccgccg ttatgagggc
     3901 gtttctgtgg aagacctgcg ccgtctcaac cgggtgatgg gcaaccccgt gttccgcgac
     3961 cgggtagtga aaatcaaaga attatagcca caaggtgcga cgtcgccatt ttcatcgcat
     4021 attgggagcc ggagttgccg gcgcagcctt ggcgcccttt gcctgcgctg ctgttcggga
     4081 tgttcctgtt tctgagaatg ttatcaagcc tcgtcgcctg aagcagggag acactgtggc
     4141 actcatttct cccggcagct atgcccccga tgacgctgtt caaaaggcgt atctcaatct
     4201 cgaaggtttg ggtctcaaag tgaagccggg caaatacttg agagcacagc gcggcttcac
     4261 ggccgggtcc gatcagcaac ggttggacga tctgcacgca gcattttcag atacagaagc
     4321 gagcgcggtg tggtgcgttc ggggaggata tggctgcacg cgtttgctgc cgtttattga
     4381 ttacccgctc atccgtcaaa accctaaaat actgatcggc tacagtgata ttacggcctt
     4441 gcttcaggcc attcatatag aaaccggctt ggtgggtttt catggcccgg tggcttcggc
     4501 tactttcaca gactatgcca aagggcagtt ggtaaaagta ttgatggaac ctgctgctat
     4561 gcacaccatt cccgttgcag aaagtcaaaa agaaaaaaaa gattctgaat atcagcctgt
     4621 tactattaat gagggcaaag cggagggcat cttgtcgggt ggcaatttgt ctttgttggc
     4681 ggccatggcc ggcacgccgc acgcgtttga ttttaccaac aagctcgttt ttatagaaga
     4741 agtaggcgag cgtccttacc gcttggatcg catgttgacg accctcttac aagcatccaa
     4801 cctgcgaaag gcagccggtg tggccctggg ggtatttacg gattgccagc ctaaaaaaga
     4861 cgaactttcg ctttctatga tggacaccct gcgcgacagg ctcgaaggct tgggcattcc
     4921 tgtggtttat gggctttcgt ttgggcatat ttccgacaac tgcacgttgc ctgtaggcat
     4981 ttccgcctca ttggatgttc agcaacgcag cattacgatt ctggaaaccc cggttctctg
     5041 atgtcatttt gacgcctgca tgatgcgttt gacctcttcc gaaagtgcgt cgggctgtat
     5101 gggtttgcct acataacctc tgaatcccga attaatacac aagtcttttt cctccggtga
     5161 aggagtggag gtggtggcta tgacaggcgc ctgattgtgt tttttcagcg cagccagcgt
     5221 atcgaatccg ttcatgtcgg gcatttgcaa gtcgagcaaa atcagatcga atttatgctg
     5281 ccccgcgata gaaaccgctg ctttgccgcc accggccaat tctacggttg catcggccat
     5341 cacattcttg acgacgtttc tgatagacaa caggtgcatg cggttatcat ctacaatgag
     5401 aatggaaggc gcgaccggca aaaagagatt cgcgccgcca tccgcttttg tttcggggat
     5461 ttttactgga atggacagtt caaaagattc gcaaagcccc ttttcgtctt ttcggagggc
     5521 atatttcccg ttgagcgctt tgagtaattg tcccgatacg gccattgtag tggcttttcg
     5581 gcgaagatcg ggtgtagaga ccattgaaaa atctgcggca tccataacgg ctttgagcat
     5641 atcggcaggc atttgcttgt cgttgggaag atactcaatc ttcatagaat gctgatcacc
     5701 ggaaacttta gagatgttaa cctgaatgac aatggtgcca ggtcggcctt taggcgcgag
     5761 ggcgcatttg aaaaaactac acaggagtgt atgtagtaag tgctcattgc cgatgagtgt
     5821 cgtgggaaaa tcggctggga ggctgagatg cactttacac ccggattttg cggcctgagc
     5881 ttcagtaact tcttgaaaat gagtaaaaaa ggaattgaat acaaaaggcg cagcttcttg
     5941 ggcggatgcg ttgcgaatgt ctgtttgcgc catcagcagg gtattcacag ccaatgaaat
     6001 ttcattgaga ccacttttga actccggcaa caattcctgt tgagatgggg tagctgtttt
     6061 ttgtaataaa tgggccagat caagggcctc aaacagcggt ttttgaatct gctctttcag
     6121 ttcggcgagt atttcctttc gccatagctc ggagacgcct ttagaagaac ccgaagacag
     6181 gatgggcgtc atttgttcag acacatcctg tgcgcttccg atgagcaaaa cctgacctga
     6241 atcctgatct aattgaactt tggcccgcag agaaaggtgt ttcacccggg tgcctacaac
     6301 tattcgatgt tctatttttt gtatttcttc ggcctgtagt gctgttgtga aaaaggtttc
     6361 cacagcttct ttgtcgtcaa agaaaacgta ttgcaaataa tctcccagag aaagggctgc
     6421 ctcggtttgg ggcaggccga aaatacgata catttcctcg ccccatttca tggcattggt
     6481 aaccacatcc atctcccaat tggcaaaatt ggctaattct tgcgcttcct gaagtctttt
     6541 ttgctgatga gatagttttt gtgcagattc ctgtagtttg gtttgtgttt ggaaccgttg
     6601 ctgagcatgg cgaatacttt tgaccagcgc tttgctgtcg aattcgcctt tgaccaaaaa
     6661 atcctgagcg ccggccctga cacactgaac gccaagtatt tcgttgttca ggccagtcat
     6721 tacaatgacg ggcacattag gaaattcggt caggaatttt tctaaggtac tgaatccgat
     6781 gctatccgtg agtgtgaggt cgagtaagac gagatcaatg gagtgttcgc gcataattcc
     6841 aaagccgcgc ttcagagaat cggcctgaaa gatcgtatgt ccgaaagcag tttcttcaaa
     6901 ataaatatga atgatggccg catccgccgg gttgtcttct atgaccagaa tgcgctgctg
     6961 catacaaccg ttgtgtaaat tattgttgga atgtggtgaa tggcggcccg aaggcgtcgc
     7021 actatcgctg caaaattaga gaaaaaaatg gagattcaat tgtgttgagg cggcaggatg
     7081 gccgtttgaa gccaaaacga attgattctg tccactattt ccaaaaaatc gtcaaaatcc
     7141 atcggtttga gcaggtagga attggcattc aattggtagc tttccaaaac atcctgctct
     7201 gatttggaag tagtaaggat cagcacagga atgcgcttaa attcaggatg gtttttgatt
     7261 ctgtggagta cttctttgcc gctccatttg ggcaggttga ggtcgagcag aatgagatcg
     7321 ggcagcgacc ggacgttttt ttcaagaaaa gccacagctt cttgcccgtc catggctaca
     7381 tcaagtgtgg caggcactcc ggcctcgtga aaagcctcct gcgtcagtcg aatatctccc
     7441 ggattgtcct ctacaagtag tatgtgtatg tgcttcattt tgtttggcag tttggcccac
     7501 aaatagtgag cgtttggccg gcagggggag gccggattga aaacaaaaat cgaagataag
     7561 aagttttgct gcaaacaatg tacaaccatg cagaaaaatc agtctcaact tgaacagttt
     7621 tttacagagt accttgaaaa tcaggttttt gatcgggagc ctcatacttt atacgctcct
     7681 attgactata tcatggcttt aggaggaaaa cgcctgcggc cgcttctgac cctgatgggg
     7741 tgtcaaatgt ttggccgccc tgccggagat gctttgccgg ctgcccttgc cgtagaggta
     7801 tttcataatt tcagcttggt gcatgacgac atcatggacg aggccccgct gcgacgcgga
     7861 atgcctaccg tacaccaaaa atacaatgcc aacacggcca ttctttctgg tgatgccatg
     7921 ctcattgaag cctaccggtt tttgtgccaa agccctgccg atgcttccct gccccgcttg
     7981 atgcacatat tcaaccatac agcgctggag gtgtgtgaag gccaagccgc agacatggac
     8041 ttcgagaaac gcgatgatgt gtcgctgccg gaatacctgc gcatgattga acaaaaaacc
     8101 gcagcgctca ttggcggcgc tctggcgctc ggtgcggtaa tcggcggggc atcggaggaa
     8161 gacgcggcgc atttggatgc ctttggccgc aacatcggca ttgcgttcca gatacaagac
     8221 gacttgctgg atactttcgg cgacccggaa aaatttggta aaaaaaccgg cggcgacatt
     8281 gcccgcaata aaaaaacatt tctgctgctc aaggctctag aatcggctac gccggcattg
     8341 cgacaggaac tgatggcttt gtacagcgaa acctcgccgg cgctcgaatc cgccaaaatt
     8401 gaacgagtga aagctgtttt ctctgaattg caaacagcgg cttcggcaca ggctgccaag
     8461 accgattacc agcagcgggc cttccagcat ctggcggcca ttgatgcccc ggaggaaggc
     8521 aaaactgttg tcagagattt tgccaattgg ttgttttcca gagatgtata aagaaaaaga
     8581 gggggtataa aaaaggagcg atcgcaaaaa cgatcacccc tcacactatg aaacactata
     8641 ttgatctttg cccgataagt ggttcgggcg cgtatgcaat tcttttgaat gtgtaaaggt
     8701 atgggctttt tgagggtatt tcaaaatttt ttcatataaa tttattttat cattgtgtaa
     8761 atcttgccgt tccaccagtt ggtttctatc gcggcatcgt attttttgta aaaattcagg
     8821 gccggttcgt tccaatccaa tacctgccat ttgacaaccc ggcattcctg cacccggcat
     8881 tcttcgagaa acgcatcgaa caaaatgcgc cctataccct gtccccggcg gctgtcggtt
     8941 actacaaaat cttccagata catcatgcga cctttccaag tggaataggc aaaatgatac
     9001 agcgcaatgc ccacgatttg cccggcgtct tcggccagga tgnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnc cggcctcaaa gtcgcggcga tagtcttcca cgcttgcggt cagttcagtt
     9121 tcggcgtgtt catacacggc cagttctcgc accaaatcat gcacggcggg caggtcttgg
     9181 agggttgctt ttcgggtagt tatcatcatg gaattaaaga ttgcggtgtt ctaacaactg
     9241 ttctaaaaaa tgagatgaaa taccaaacat ctgcttgatt tcctgcactt tggcccttgc
     9301 atctgcgtca ggttgagcgc gggtagcggc aatcatcagg tttttgttgg tatgttctat
     9361 ggagacaaac tcaaacactt tggtctggta gccttccgcc tctaaaagca gagagcggat
     9421 gctgtcggtg agcagttcgg cctggcgctc ttccaaaata ccgtgccgga gcatggtttt
     9481 gaggatgccc tgcggctgaa tttgctgcct gatttgtttg tgacaacagg gtgctgcaag
     9541 aatgatgccc gctccggccc tgatgccctc ggccagcgcc acatcagtga gggtgtcgca
     9601 ggcgtgcagg gcgatgagca tatccgtgct tttgccttca aaatcgcgga tgtcctgagt
     9661 ttggaagcgc agcccttcga agccgcactg ccgggccagg tggttggcaa actccacgag
     9721 gtgcggcctg atttcgagtc ccagcacctg aggctccatt cccagattgt ttttcagatg
     9781 atcgtacagg gcaaaagtga ggtaaccttt cccggcgccc atgtccacta tatgggcttg
     9841 gcggggcagg ggatgtttgc gcaa
//
LOCUS       DAORLF010000139         9837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3699_length_9837_cov_2.114189, whole genome shotgun sequence.
ACCESSION   DAORLF010000139 DAORLF010000000
VERSION     DAORLF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_9837_cov_2.114189"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>378)
                     /locus_tag="PK198_12275"
     CDS             complement(<1..>378)
                     /locus_tag="PK198_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39555.1"
                     /translation="VPVILGNVHALPMRDDADVDAPFKLAAQLQQAGVMFCFSMDGFW
                     QQRNLAFQAGHAVGFGLPYEQAVAALTLNTARILGIDATVGSIEEGKDAILFLCEGDA
                     LDMRSSKVSLAFINGRQISLDHK"
     assembly_gap    379..400
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<401..1243)
                     /locus_tag="PK198_12280"
     CDS             complement(<401..1243)
                     /locus_tag="PK198_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39556.1"
                     /translation="MQRNFLFCILSVITFSVYAQTPVPAPPQEMPVYIVGATAHLGNG
                     LVVENSIVAFENGKITLVRTPQTLPQVNLSRYKVIEAKGKHLYPGFIAPNTQLGLVEI
                     EAVRATVDAGEIGELNPNARSIIAYDSDSEVPPTVRAQGILLAQITPQGGLLSGQSSI
                     VQLDAWNWEDAAYKMDIGLHLNWPAMFSFNWREGSLSKNANYDKQVEVIETYLTEALA
                     YCKNDKPAEKNLKLESACALFNGSRKLFLHADDAKAITAGVLLAKRFGITPVVVGGGD
                     AAIIA"
     gene            complement(1271..2368)
                     /locus_tag="PK198_12285"
     CDS             complement(1271..2368)
                     /locus_tag="PK198_12285"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRF39557.1"
                     /translation="MAVETTQDEAKRCTNCEHPMDEGAKFCSACGQKRVPGIPTVWHL
                     VKDFLASLLNLDSQLFQTLRDMMIPGKLTTEYFKGRHKRYINPARLFLVTALLHFAVL
                     GYIFSGGQEIMTELLEQQKRSGHRDLFLLELDSVKSEIAAGYKKYPLVGEALDSLRAH
                     MGDTVSLDSSTFGYYSVQKGEFVELTVSSQDMLDLRTQDLLDKYHVQGRMERFQAGQF
                     YRILREGDSFLSYLLGNLIWLVALMMPALALLLKLLYIRRRRYFVEHLVFSFHYHAFA
                     FVVFSIAFFFFGSDMLAAFQSEKDVNLGVHPAHSIALALVLIYLFVAMKRVYQQGFIK
                     TFLKFWVLNVSYLFIFTLCLTLTLVVGALLF"
     gene            complement(2521..3504)
                     /locus_tag="PK198_12290"
     CDS             complement(2521..3504)
                     /locus_tag="PK198_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF39558.1"
                     /translation="MRKEKFVYNTHTLRYEKVVEPWSAIILRIFGFVCAALVTGFIFT
                     LVSHRFFPSPKEKALLNEIQQQKALLGDASEELDRLRTQLGSLKDRDEYAYRMIFGME
                     PIDEAVWEGGVGGHDQYEQLKEFRYSGETMASVQEKIDKLKRQMVIQSKSLDTIMDMS
                     AAKEKMLASMPSIKPVRADKLNRGVSLLSGFGYRIHPIYKVPRMHTGIDFSAPKGTSI
                     QATGAGKVVKAENEGNGYGLCVTIDHGYGYQTIYAHMSKIDVKVGQKVVRGQSIGRVG
                     SSGASTAPHCHYEVVYKGNKVNPIHYVMDGLSPQEYQTLVKASETANQSFD"
     gene            3995..>4640
                     /locus_tag="PK198_12295"
     CDS             3995..>4640
                     /locus_tag="PK198_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39559.1"
                     /translation="MRFTTILLLLAGLFGASVAVAQNGIIKGKVTNKINREPISFANV
                     LVENTDKGAVTDENGAYEITGLAPGLYNVRVSFIGFADVAEFEVQVANTRPAEVNLEM
                     EENAAALTEVVVKSSPFRKTEESPVSLRTIGVAEIMRNPGGNRDISRVVQALPGVTTP
                     ASFRNDLIIRGGAPNENRFFLDDVEVPVINHFATQGASGGPVGIINVNFIQEVDF"
     assembly_gap    4641..4671
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4672..>4966
                     /locus_tag="PK198_12300"
     CDS             <4672..>4966
                     /locus_tag="PK198_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39560.1"
                     /translation="SSSVFNFRQRDGRNDRIGGTFQVSATDIGATLEGPIGDKTTFLV
                     SARRSYLQFLFKAIGLPFLPTFNGFQAKIKTKFDERNELYFIGLGAVDQFKLN"
     assembly_gap    4967..5022
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5023..6403
                     /locus_tag="PK198_12305"
     CDS             <5023..6403
                     /locus_tag="PK198_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39561.1"
                     /translation="QWNYTNGLVYKHYTDNGFWTVVLSRNMLNNESEKYFRNDDSTED
                     NLIFRYRSQEVENKLRIENTSRIKGFKLNYGAGYEFVRYNNRTFNRIFTAAGPQTVNY
                     ASDFNLNKYYAFAQLSRKFLDERLVLSGGIRVDGNDFSEAMSNPLDQFSPRFSASYNF
                     TDRLAFNFNTGIYYQLPPYTLLGYQEDGVFVNRDNGAKFVRNDHLTAGLEWNTASNTR
                     ITVEGYYKKWYDYPFLLRDSLTLANLGGDFGVLGNEPSVPRSDGRSYGLEILLQQRLY
                     KGFYGIISYTLGWSQFQDKNGQFVASSWDARHIVNTAVGKKFKRDWEVGINWRFQSGL
                     PFTPFDEASSLRANWDVNNRALVNYNLLNTLRRTAANTIDIRVDKKWFFKKWSLNLYL
                     DIENITGNSISSDQLILDRPLDENKRPIGPGVVVNPDAPYNEQRYLLKTISNAQGTVL
                     PSIGIMVDF"
     gene            6570..7487
                     /locus_tag="PK198_12310"
     CDS             6570..7487
                     /locus_tag="PK198_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF39562.1"
                     /translation="MAIYAIKDLEKLSGIKAHTLRIWEQRYGLITPKRTPTNIRYYDD
                     EDLKFILNIALLNRNGIKISKIAQMSKSEITGKVSELSDFRFEEGAQLDALTLSMIEM
                     DEYKFERIVVTNIQQIGFERTMLEVVYPFLDKLSLLWLTGSIHPVQESFISNLIRQKL
                     IVAIDRLPMPTGRSVQKLLLYLPEGERQELTILFMQYLAKARGYHVVYLGLDVSLTDL
                     RDAQRILQPHFVFTMITETFGKEPVQRYVDKMADVFPDSRVLLSGYQVIAQNVVSPSN
                     TIILNSLEHTLSYLESAVLPQPARQGSLV"
     gene            complement(7503..>8604)
                     /locus_tag="PK198_12315"
     CDS             complement(7503..>8604)
                     /locus_tag="PK198_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF39563.1"
                     /translation="PLSKYWQLQAGAKISTTRLDNKLLASLMQNGEWVNDALRTNTFL
                     YDEDIYAGYTNLTFNREKLQANIGLRYEDSQAVGFSETLDSTVNLRIKRFFPSLSVSA
                     PLGGSLGWSAAYSYRIKRPGYYDLNPFIQYLDPLTSDKGNPFLVPELTHSGQLSLTFD
                     KQPFFNLSYDVTRNALTEVIEQNDETGEAFKTNVNLDKYTRYGGSLFFPLDFIAKSIS
                     GYGGVMVYYHDYRAEYLNADFLQNQWSATGFLQVNATLPKGWKAELSGWIQSPGIDGI
                     MRHETMYGVSAGLQKELFEKRLRLQLNAEGIVQKFFNARVDYANLDFDILSQWEAPVV
                     NLRATWKFGNQHLKNRDNRDSSSSSEQRRLNN"
     assembly_gap    8605..8619
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8620..>9837)
                     /locus_tag="PK198_12320"
     CDS             complement(<8620..>9837)
                     /locus_tag="PK198_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39564.1"
                     /translation="VVQAYSQSAVFGQIISEKGEPLEFINVLLLNAADSAIVKMELSD
                     ALGKYAFRQVAPGEYRVKTSGLGYSDLLSASFSVAKGEDATVPVMRMAIAAEVLQTIE
                     VVARRPLLEQQAGKLVVNVEDNITGLGGSVTDVLRKVPGLLVINDRVQMAGKSGVTIL
                     IDGRPTKYMDVESLLRDMPADNIARIEVVSQPGAAFDAEGTGGVINIVLKKNALLGTN
                     GQIMLGMGRGELWKYRGNASINHRSGPWNLTAGAGYNHNTFIERLDLVRRLPNQHFVQ
                     SAYTPGNPVSKSLRLGADYDINDRHRVGITMNTVYSTNRRTGENTTYVTTPEGAELSR
                     FTTLNVQDRNWRSYSGDAFYRWKIDTSGQEFSMDYNYAYYRRRVQSDLLTTGADFEDR
                     RNVEPADTRIFAA"
BASE COUNT         2287 a         2617 c         2457 g         2352 t
ORIGIN      
        1 gcttgtggtc caggctgatc tgccgcccgt tgatgaaggc caatgaaacc ttgctgcttc
       61 gcatgtccaa cgcatcgcct tcgcagagaa acagtatggc gtctttacct tcctcaatag
      121 aaccgacggt ggcgtctatg cccaaaatac gggccgtgtt gagtgtcagc gccgccactg
      181 cctgctcgta cggaagcccg aagcctacag cgtgtccggc ctgaaacgcg aggttgcgct
      241 gctgccagaa accatccatg ctaaagcaaa acatgacgcc tgcctgctgc aattgggccg
      301 ccagtttgaa cggcgcatcc acgtcggcat cgtcccgcat gggcagcgcg tgtacgttgc
      361 ccaaaatgac cggcacatnn nnnnnnnnnn nnnnnnnnnn ggctataatg gccgcatcgc
      421 cgccgcccac tactaccggc gtaatgccga aacgcttggc caacagcacg cctgcggtga
      481 tggctttggc gtcgtcggca tgaagaaaca gcttgcgact gccgttgaac agggcgcagg
      541 ccgattccaa ttttaggttt ttctcggcgg gtttatcatt tttgcaatag gccagcgctt
      601 cagtcaggta agtttcgatg acttctacct gcttgtcgta gttggcattt ttgctcaggc
      661 tgccttcccg ccagttgaac gaaaacatcg ccggccagtt gaggtgcagg ccgatgtcca
      721 ttttgtaggc ggcatcttcc cagttccatg cgtccaactg cacgatagac gactgccctg
      781 aaagcagacc tccctgcggc gtgatctgtg ccagcaggat gccctgcgcg cgcaccgtag
      841 gcggcacttc agagtcggag tcatacgcaa tgatggagcg ggcattggga ttgagttcgc
      901 cgatttcgcc ggcgtccacg gtagcgcgca cggcttcaat ttctaccaat cccaattgcg
      961 tattcggcgc gatgaagccc ggatagaggt gtttgccttt ggcctcaatg actttgtagc
     1021 gactgaggtt gacttgcggc agagtttgcg gcgtgcgcac cagcgtgatc ttgccgtttt
     1081 caaaagccac aatgctgttt tccactacta acccgttgcc caaatgcgcc gtagcgccta
     1141 cgatgtaaac cggcatttcc tgtggcggcg caggtacggg cgtttgagca taaacgctga
     1201 atgtaatgac ggagaggatg caaaaaagaa agttgcgttg cataacggtc tgtgatgagt
     1261 ggatgagaaa ttaaaacagc agcgccccga cgaccaaagt cagggttaaa cacagcgtaa
     1321 aaataaataa ataggagaca ttgagtaccc aaaatttcag gaaggtcttg atgaagcctt
     1381 gttgatacac ccgcttcatg gctacaaaca gataaatcag taccaatgcc aacgcaatgc
     1441 tgtgagccgg atgcacgccc aagttgacgt ctttttctga ttggaaggcg gcaagcatat
     1501 ccgacccgaa aaagaaaaac gcaatagaaa acacgacaaa tgcaaaggca tggtagtgaa
     1561 aggaaaaaac cagatgctct acaaaatacc gccgcctgcg gatgtacaac agcttgagca
     1621 acagcgccaa agcgggcatc atgagcgcta caagccaaat gagattgccc agcaggtagc
     1681 tcaaaaaact atccccctct cggagtatgc gataaaactg cccggcctga aatcgttcca
     1741 tacggccctg cacatgatac ttgtcgagca ggtcttgagt gcgcaaatcc aacatatcct
     1801 gtgagctgac tgtcaattct acaaactctc ccttttgcac gctgtaatag ccaaaggtac
     1861 tggaatctaa agaaacagta tcgcccatgt gggcgcggag ggaatccaac gcctccccta
     1921 cgagggggta ttttttatac ccggcggcta tttccgattt tacgctgtcc aattccagca
     1981 aaaacagatc gcggtgcccg cttctctttt gctgctccaa taactccgtc ataatttcct
     2041 gcccgccgga aaatatgtat cccagcacgg caaaatgcag caacgcagta acaagaaaga
     2101 gccttgccgg attgatgtag cgtttgtgcc gccctttgaa atactcggtg gtcagctttc
     2161 ccggtatcat catgtcgcgc agcgtttgga aaagctggga gtccagattg agtaaagagg
     2221 ctaagaaatc cttcactaaa tgccagacag tcgggattcc gggcacgcgc ttctggccgc
     2281 aggcactgca aaacttagcc ccctcatcca tggggtgctc acagttggta caccgtttag
     2341 cttcgtcttg agtcgtttcc actgccattg gttctttttt gttcaaaagt gtgacaggag
     2401 caggtttggt gcgccgattt tttcgacgag cgcccggttt tgtttctctt tacgctgtca
     2461 acgaaaaaag gcaagaccac gaatccagcc ttgccttttt tgccgcgatg caacgcgtga
     2521 ttagtcgaac gactgattgg ccgtttcgga ggcttttacc aatgtttggt attcttgagg
     2581 agacaggccg tccatcacat agtgaatcgg gtttacctta ttgcctttat aaaccacttc
     2641 ataatggcaa tgcggcgcag tggaggcgcc cgaactacct acccgcccga tagactggcc
     2701 gcgaacaact ttctggccta cttttacatc tatcttgctc atgtgcgcat aaatggtttg
     2761 gtagccatat ccatgatcta tggttacgca caggccataa ccattgcctt cgttctcggc
     2821 ctttaccact ttgccggcgc cggtggcctg aatggaggta cctttgggcg ctgaaaaatc
     2881 aatacccgtg tgcatgcgcg gcactttgta gatcgggtgg atgcggtagc cgaaaccgga
     2941 caagagcgac accccacgat tcaacttgtc ggcgcgtacc ggcttgatgg aaggcatgga
     3001 ggccagcatt ttttctttag cggcagacat atccatgatg gtatccaaag acttggactg
     3061 tatcaccatt tgccgcttca gcttgtctat tttttcttgt accgaggcca tggtttcgcc
     3121 ggaatagcgg aactctttga gctgctcata ctgatcgtgg ccgcccacgc cgccttccca
     3181 aacggcttca tcaatgggtt ccatcccgaa aatcatgcgg tacgcatact catcccggtc
     3241 tttgaggctg cccaattggg tgcgtaaacg gtccaactcc tctgaggcat cgcccagcag
     3301 cgctttttgt tgttgaatct cattcagcaa tgccttttcc tttggcgaag gaaaaaagcg
     3361 gtgggaaacg agggtgaaaa tgaacccggt aaccaatgca gcgcaaacga agccgaaaat
     3421 tctgagaatg atcgcgctcc aaggttctac cactttttcg tacctgagcg tatgcgtatt
     3481 gtaaacaaac ttttcctttc tcatgagctt ttagaagtta acgaacgcgt tggttttcaa
     3541 tagtttttgg aatgtaatag aaggctttcc aaactttgaa attcgctttg ttgtctttgt
     3601 ccgtgttttc ttagcaggcc aaaagtaata caggaattcg atttcccaaa actttaacta
     3661 aaaaaagtaa attatattaa aattattgtt ttaaattata taaaacaatt tattttaaat
     3721 ttgtctcgca taggccctca gcaactgcca tgaccatcct ctctgcgcaa tccttacgcc
     3781 tggctgtttt tgtcagaaca gcatacagcg agcgcgagcc gaagaaaatt ttccaacatt
     3841 ttaccgcttg gtctgttcag catcctggca agagcaaaaa ccgctttcgc tgtgtctttt
     3901 taatcatcaa gtaattgaat caaatcctgt gccggacttg caaacacgtt cgctcagcaa
     3961 tcagcacaac agcacaccac tacatttact caccatgcgt ttcacaacca ttctactcct
     4021 cttagccggc ctgttcggcg cttccgttgc agttgctcaa aacggcatca tcaaaggaaa
     4081 agtaaccaac aaaatcaatc gcgagccaat ctccttcgcc aatgtgctgg tggaaaacac
     4141 tgacaaaggc gctgtgaccg acgaaaacgg cgcttacgaa atcaccggcc tggcgccggg
     4201 cctgtacaat gtgcgcgtga gctttatcgg ctttgccgac gtcgcagaat ttgaggtaca
     4261 ggtcgccaat acccgcccgg cagaagtgaa cctcgaaatg gaagaaaacg ccgccgccct
     4321 gaccgaggtg gtggtcaaat cttcgccttt ccgcaaaacc gaagaaagtc cggtgagcct
     4381 gcgcaccatc ggtgtagcgg aaatcatgcg caaccccggc ggcaaccgcg acatctcgcg
     4441 cgtggtgcaa gccctgcccg gcgtcactac gcccgcttct ttccgcaacg acctcatcat
     4501 tcgcggcggc gcgcccaacg aaaaccgctt ctttttggac gacgtggaag tgcccgtcat
     4561 caatcacttt gctacacagg gcgcatcggg cgggccggtg ggcatcatca acgtcaactt
     4621 cattcaggaa gtagattttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctccagct
     4681 cagtgttcaa tttccgccag cgcgatggcc gcaacgaccg catcggcggc acttttcagg
     4741 tgagcgctac cgacatcggc gccacgctgg aaggccccat cggcgacaaa acgacgtttt
     4801 tggtttcggc ccggcgctcg tatctccagt ttttgttcaa agccatcggg ttgccgtttt
     4861 tgcctacttt caacggcttt caagccaaga tcaaaaccaa atttgacgag cgcaatgaac
     4921 tgtatttcat cggtttgggt gcagtagatc aattcaaact caatttnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaatgga actacaccaa
     5041 tggcttggtg tacaaacact acaccgataa cgggttctgg acggtagtgt tgagccgcaa
     5101 catgctcaac aacgaatcgg agaagtattt ccgcaacgac gattctaccg aggacaacct
     5161 catttttcgc tatcgttcgc aggaagtgga aaacaaactg cgcatcgaaa atacttcccg
     5221 tatcaagggt ttcaaactca actatggcgc gggctacgaa tttgtgcgct acaacaaccg
     5281 tacgttcaac cgcattttca ctgccgccgg cccgcaaacc gtcaactacg catctgattt
     5341 caaccttaat aaatattacg ctttcgcgca attgagccgc aagttcctcg atgagcggtt
     5401 ggtactctcc ggcggcatcc gcgtggacgg caacgacttt tccgaggcca tgtccaatcc
     5461 gcttgatcag ttttcgccgc gcttttcggc ttcctacaac tttaccgacc ggctggcctt
     5521 caatttcaat accggcattt attaccaact gccgccttac acattgctcg gctatcagga
     5581 agacggcgtg tttgtgaacc gcgacaacgg cgccaaattt gttcgcaacg accatctcac
     5641 cgccggcctc gaatggaaca ccgcctccaa cacccgcatc acggtggagg gttattacaa
     5701 aaaatggtac gactacccat tcctcctgcg cgacagcctg accttggcca atcttggcgg
     5761 cgacttcggc gtgttgggca acgaaccctc cgtaccgcgt tccgacgggc gcagctatgg
     5821 tctggaaatc ctgcttcagc agcggttgta caaagggttc tatggcatca tttcctacac
     5881 tttgggctgg agccagtttc aagacaaaaa cggccaattc gtggcttcct cctgggatgc
     5941 gcgccatatt gtgaatacgg cggtgggcaa aaaattcaag cgcgactggg aagtgggtat
     6001 caattggcgt ttccagagcg ggctgccgtt tacccccttc gacgaggcat cctcattgcg
     6061 cgccaactgg gatgtgaaca atcgcgcact cgtcaattac aacctcctga acaccctgcg
     6121 ccgcacggct gccaacacca tagatattag agtggacaaa aagtggtttt tcaaaaagtg
     6181 gagcctcaat ctctatttgg acatcgaaaa catcaccggc aactccatca gttccgacca
     6241 actcatcctc gaccggccat tggacgaaaa caagcggccc atcggccccg gcgttgtcgt
     6301 caatcccgat gcgccctaca acgagcagcg ctacctcctc aaaaccatca gcaatgcgca
     6361 gggcaccgtg ctgccgagta ttggtattat ggtggatttt tagataaagg aggggtgagt
     6421 gattttgcca tccaactgca caattactca atttttaaat acactctctg ctctattggc
     6481 ttacggtacc ctgtgcctgt aaaccttttc agtgcttctc tgtttcgatc ttgtttattt
     6541 tcaagccttc accagaatta tcgtcaggcg tggccattta tgcaatcaaa gatttggaaa
     6601 aactgtcggg catcaaggcc cacacgctgc ggatatggga gcaacgctat ggcctcatta
     6661 cgcccaagcg cacgcctacc aatatccgtt attacgatga tgaagacctc aaatttattc
     6721 tcaacattgc actactcaat cgaaacggca taaagatttc aaagatcgcc cagatgagca
     6781 agtcagagat cacgggaaag gtatccgagc tgtccgattt tcgttttgaa gaaggcgcac
     6841 agttggatgc cctcacgctc tccatgatcg aaatggacga gtacaagttt gagcgcatcg
     6901 tggtaaccaa tattcaacag attggttttg agcgcactat gctcgaagtt gtctatcctt
     6961 ttttagataa gctgagtctg ctttggctca cgggatccat acatccggtg caggagagtt
     7021 tcatcagcaa cctcattcgc cagaaactca ttgtggccat agaccggctg ccgatgccga
     7081 cagggcggag cgtgcaaaaa ctgctgctgt atctgccgga aggcgagcgg caggaattga
     7141 ccatcctgtt catgcagtat ctggccaagg cccgcggcta ccacgttgtg tatttaggat
     7201 tagacgtgtc tcttaccgac ctgcgcgatg cacagcgcat cctccagccg cattttgttt
     7261 ttacgatgat tacggaaacc ttcggcaagg agcctgtgca acgatatgtg gataaaatgg
     7321 ccgatgtgtt tcccgacagc cgcgtattac tctctggtta tcaggtcatt gcccaaaatg
     7381 tagtttctcc ctccaatacg ataattctca atagcttaga acatacccta agctatctgg
     7441 aaagcgcggt actgccccag ccggcacgcc agggttcctt ggtctgacgt tttactcctc
     7501 ctttaattat tcaacctccg ctgctccgac gatgagctgc tgtcccggtt gtcccggttt
     7561 ttgaggtgtt ggttgccgaa tttccaggtg gcccgcaagt tgaccaccgg cgcctcccat
     7621 tgagacaaaa tatcgaaatc gaggttggca tagtccacgc gtgcgttgaa gaatttctgc
     7681 acaatgcctt ctgcgttgag ttgcaggcgc aggcgttttt caaaaagctc tttttgcagg
     7741 cccgcactca ccccgtacat cgtttcgtgg cgcatgatgc cgtctatacc cgggctttgt
     7801 atccagcccg acagctcggc tttccagccc ttgggcaggg tggcgtttac ttgcaggaag
     7861 ccggtagccg accattgatt ttgaaggaaa tctgcattca ggtactccgc tctgtaatcg
     7921 tggtaataca ccatgacgcc gccgtagccg gagatggatt tggcgatgaa atcgagcgga
     7981 aaaaacaggc tgcccccgta gcgggtgtat ttgtccaagt ttacattggt tttgaaagcc
     8041 tcgccggttt cgtcgttttg ctcgatgact tccgtgagcg cgtttctggt cacatcataa
     8101 gacaaattga aaaacggctg cttgtcgaac gtgagcgaaa gctggccgct gtgcgtcagt
     8161 tcgggtacca gaaacgggtt gcctttgtcg gaagtgagcg gatcgaggta ttggataaag
     8221 gggttgagat cgtaataacc gggccgtttg atgcggtagc tgtatgccgc gctccagccc
     8281 aaagaaccgc ccaatggcgc cgacacgctg aggctgggga aaaagcgctt gatgcgcagg
     8341 ttgacggtag agtctaatgt ttcgctaaag cccacagcct gcgaatcctc gtagcgcaaa
     8401 ccgatattgg cttgcagctt ttcgcggttg aaagtcaggt tggtatagcc cgcatagatg
     8461 tcttcatcgt acaaaaaagt attggtgcgc agcgcgtcgt tcacccattc tccattttgc
     8521 atcaggctgg ccaagagctt gttatccaaa cgcgtggtgc ttattttggc gccggcctgc
     8581 aactgccagt acttcgacaa aggcnnnnnn nnnnnnnnnt gcgctgcaaa aatgcgcgta
     8641 tcggcaggct ccacattgcg gcggtcttca aagtcggcgc cggtggtcag caggtcgctt
     8701 tgcacccggc ggcggtagta ggcgtagttg tagtccatgc tgaattcctg cccggaggtg
     8761 tcaattttcc aacggtaaaa agcatcgccg gaataagacc tccagttgcg atcctgcacg
     8821 ttgagggttg taaaacgaga gagttctgcc ccttccggcg tcgtgacgta ggtggtattt
     8881 tcgccggtgc ggcggttggt gctatatacc gtgttcatgg tgatgcctac gcggtgccgg
     8941 tcgttgatgt cgtagtcggc gccgagccgc agcgatttgg acacggggtt gccgggcgta
     9001 taagccgatt gcacgaaatg ttggttcggc aggcggcgca cgaggtccaa gcgttcaata
     9061 aaagtattgt gattgtagcc cgcgccggcc gtcaggttcc aggggccgga gcggtgattg
     9121 atggaggcgt tgccacgata tttccacagt tcgccgcgcc ccatgcccag catgatctgg
     9181 ccattggtgc ccagcagtgc attttttttc aaaacaatat tgatgacgcc gcccgtgcct
     9241 tcggcatcaa aagcagcgcc cggctgcgat accacctcta tgcgggcgat gttgtcggcg
     9301 ggcatatcgc gcagcagcga ctccacgtcc atgtatttgg tggggcgccc gtcaatgagg
     9361 atggtgacgc ccgatttgcc ggccatctgc acccggtcgt tgatgacgag gaggccaggc
     9421 accttgcgca gcacatcggt gacgctgccg cccaagccgg tgatgttgtc ttccacattc
     9481 accaccaatt taccggcttg ctgttcgagc agaggacgcc gggccaccac ctcaatagtt
     9541 tgcaatacct cggcggctat ggccatgcgc atgaccggca cggtggcatc ttcgcctttg
     9601 gcgacggaaa aagaagcaga gagcaaatcg gagtagccta agccggaagt ttttacccgg
     9661 tattcgccgg gcgctacctg ccggaatgcg tacttgccca gcgcatcgct gagttccatt
     9721 tttacaatag cggaatcggc ggcattcaac aacaagacgt tgataaattc caagggttct
     9781 cctttttcgg agatgatctg gccgaacacg gcactctggc tgtacgcctg aacgaca
//
LOCUS       DAORLF010000140         9826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3705_length_9826_cov_1.664415, whole genome shotgun sequence.
ACCESSION   DAORLF010000140 DAORLF010000000
VERSION     DAORLF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_9826_cov_1.664415"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>974
                     /locus_tag="PK198_12325"
     CDS             <1..>974
                     /locus_tag="PK198_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39565.1"
                     /translation="QRIDCQTEEVVLGGTATTTGPHIEYLWTTSGGAFSGPDNTAMTT
                     TRTSGVYLLEVHNLQNGCSDTSNVVVETDTLAPIADAGDVFVLDCQIREAVLNGAASS
                     VGAGIEYEWTGPCIVGPANAPTTIADCPGLYTLTVNNTLNGCSASDTVSILLALATAI
                     AVVPDTLYISCETGTVVLDGSSSVYGVFEWQFNGAPSSLSEISPVVSMPGEYVLLVNT
                     LALNCPDADTTIVLLDCQISAAIAALTDTLTCAQTTATLNGSASSAGPAITYSWTGPA
                     AGCIASGQGTSMVQVVCPGEYTLTVSNTAVGISEMATVMVQADTIRPLA"
     assembly_gap    975..1007
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1008..1258)
                     /locus_tag="PK198_12330"
     CDS             complement(<1008..1258)
                     /locus_tag="PK198_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39566.1"
                     /translation="MHGKTVFPKLTTGGSAVLEMVTKSTAWQPLTVLVTDRKYKPGTV
                     AVSEGKALTNAPPASVHSYIIPAPVDEALPFRRAKGRIQV"
     assembly_gap    1354..1387
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1388..>2425
                     /locus_tag="PK198_12335"
     CDS             <1388..>2425
                     /locus_tag="PK198_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39567.1"
                     /translation="YIIEVLNMATGCSIRDSVWVTQPECGPCIAVSPPDTVTCQVTSV
                     VLNAAFCEPCPGCTVQWSVIAGAILSGAGTLMPEVRAGTYTLSATDTAGVITTLTVEV
                     PADTLPPAADAGPDRIITCEDAVVMLGAGPVPSPEYRYRWLQSNVSPGQNQLLVTVQT
                     PGFYVLEVENMANGCVASDTVLVTIDTLRPVAEAGPDQVITCGMPFAIPDAAGSTLGN
                     NIAYAWSTTSPGIIAAGENTLNPIITAAGWYRLLVTNTLNGCTAEDSLQVTLSAQLPF
                     VPDVPDMTLTCSDTVLLLNPILPDTAGLSFQWCRMVGTMPTDCSVGRTVSINMPGTWR
                     FEVLNTSTGCR"
     assembly_gap    2426..2467
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2468..4501
                     /locus_tag="PK198_12340"
     CDS             <2468..4501
                     /locus_tag="PK198_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF39568.1"
                     /translation="PVAEAGASPLPLNCTRLSVPLAGMAGPAGVPVEIAWSTSGSGTA
                     MPDNVLNPTVTSPGIYTLTVTRTDNGCSASDSVEVLLNDNFPVANAGPDTSLTCRVTE
                     LRLQGSAQTIGAPAQWNWSSPDATILEGAQTPNPRINQAGLYILTITDPANGCTDTDT
                     VVVIANQLPPDIDIQAPEGWTLNCLHPTLVLDASGTTSSGPVRFQWFGTPGSIDGRVD
                     SSAIRANSVGFYRLVVEDVIRGCRDTAILQIGGDFAVPSVAVAAPLVITCARPEVTLT
                     AIPSPPGDFYTTRWLGPSGDTVAGAGLTAAVRLAGAWRVWVTDTRNGCFGIGNIMVAE
                     DLVPPVARIAAPDTLDCTTRSVLLNGSASVGRGALAFEWSGPSGGITTGNMAVSATAM
                     LPGVYFLEVTDALNGCRDTAEVLVVETSVPITGVEAEIIPPACPGTGDGSIHLLNIEG
                     GTGPYGITFNGSGPSLTRDFYYLSPGVYTVRIQDALGCSLDTSFNLLDPPLLTVDLGA
                     DLLIRLGDSVRLEAMANLPVTAYRWAPAEAFSDAGAAVQLVTPLFTTTYQVTVTNAEG
                     CTATDWITLTVNTLNLYFAPDAFSPNGDGNNDYYTIFAAPAIKEIQSLHIFDRWGNMV
                     FSRSNFPPNDPALGWDGNFEGRPMNAAVFVVMAEIVLPDGRLELYKGELTLLR"
     gene            complement(4540..>5763)
                     /locus_tag="PK198_12345"
     CDS             complement(4540..>5763)
                     /locus_tag="PK198_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39569.1"
                     /translation="KTSTALDNHRLSILCHLRMGELQMQTNTYADAEKSLRQAHLLCI
                     ESASNSILMEVLERLAQLQQLKGNYEASNAYLREYIMMKDSLINESSNNQIIKLNTLY
                     EVGKKENAIKVLQQEKELAEIRRKGNQRTMIGLSVFLAALAGLLIWVLILVRRQRESN
                     KVLQELNGQIESQNLTLQSYNEELRQYAYVASHDLQEPLRTIGSFVGIIKRKYTDQLD
                     EQAQQYMDFVTNGVARLQLLLKDLLAYTRIERENTDFAKVETTEVVNEVIGSLHQVIQ
                     DSGARIDLDVASMPAVMGNRSRLGQVFQNLISNGIKFRGEQAPVVEVGCMPDPDKKEY
                     LFYVKDNGIGIPEEFRVKMFEMFTRLHSRDAYEGSGIGLATCRKIVEKHGGKIWAESE
                     AGMGSAIFFTLPFIQ"
     assembly_gap    5764..5910
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5911..>6260)
                     /locus_tag="PK198_12350"
     CDS             complement(<5911..>6260)
                     /locus_tag="PK198_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39570.1"
                     /translation="NPLLPNNKPEEQAKVLSLLGAIYEALGNYDKALEYQLKALENRE
                     KAGNAEGTAESLYKIGSMYFSQEKYDKSLEYYKKALHLCEKDISIPRKNLLNCLSAVG
                     SAYEELGDQQESLR"
     assembly_gap    6261..6303
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6304..6606)
                     /locus_tag="PK198_12355"
     CDS             complement(<6304..6606)
                     /locus_tag="PK198_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39571.1"
                     /translation="MSVNICLFRICTSLVFGILTLVSGQNSYAQADAASPRPSDGVPY
                     LMAIGDSLLSIDENKKAQGYYAQALKESEKQRNESAALSAKIKLAQTLVKLSEYNAA"
     gene            complement(6708..6929)
                     /locus_tag="PK198_12360"
     CDS             complement(6708..6929)
                     /locus_tag="PK198_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39572.1"
                     /translation="MFNFQKRNKKQAEMKPLSKEEQQSIKGGAQGNPPDWDLHGNPPD
                     WDLHGNPPDWDLHGNPPDWDVKGNPPDWD"
     gene            7610..8326
                     /locus_tag="PK198_12365"
     CDS             7610..8326
                     /locus_tag="PK198_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF39573.1"
                     /translation="MSKTLSIIIPAYNEARTIRFILDKVRMATLPQEIGREVIIVNDC
                     STDDTREAIQQYMAEYPDMGIQYREHRVNQGKGAALHTGIREASGDYIIIQDADLEYD
                     PREYTLLLMPILEGMADVVYGSRFMGGNPHRILFFWHSIGNKFLTSLSNAMTNLNLTD
                     METCYKLFRADIVKSLVLKEKRFGFEPEVTAKVARVPGVRIYEVGISYYGRTYAEGKK
                     INWKDGFRAIYCILKYNIWG"
     gene            8456..>9441
                     /locus_tag="PK198_12370"
     CDS             8456..>9441
                     /locus_tag="PK198_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRF39574.1"
                     /translation="MQGKGVVRFFLIVVALVTLAQFLLIIPTRRVEKNADAYATAASA
                     NAPAESKTAVFKEKRIAYLDSLSSEPVFTVPLIKSYTYQDLKRQQIALGLDLKGGMSV
                     VLQVDLRKFVNALANESSDADFKAALDAALDAQKNSEADFITLFADNFRSISNGKPLA
                     PIFARNEALGLNFESSDGDVVQVLRARADETVGLTFDRLKERIDKLGVVQPNISLDAA
                     RDIIVVELPGIDNPERARRFLQAAAELEFWNVYRISDPGMMEAFAAANERLAKMKGTE
                     ASTQRQILKIDTLYKVDEAGNSVVDRLDTTYAPITGGPLFDVFTINSSGSYG"
     assembly_gap    9442..9575
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9576..>9826
                     /locus_tag="PK198_12375"
     CDS             <9576..>9826
                     /locus_tag="PK198_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39575.1"
                     /translation="TTQKATDNYELYAIRKERGGDNAPLTGEVVTNASAQPDENNQMA
                     VSLQMNASGAQIWGQMTTKAAQDNNREVAIMLDSAVVSAP"
BASE COUNT         2191 a         2413 c         2438 g         2351 t
ORIGIN      
        1 caacgcattg attgtcaaac agaagaggtg gttttaggag gcaccgccac cactacgggg
       61 cctcatatag agtacctgtg gacgacctca ggaggcgcat tttccggccc cgacaacacc
      121 gccatgacca ccacccgtac atccggcgtt tacctcctgg aagtacacaa tctgcaaaat
      181 ggctgttccg atacttccaa cgttgtagtg gaaacagaca cgctggcgcc catagccgat
      241 gcaggagatg tttttgtgct ggattgccaa atcagagaag ccgtgctcaa tggcgctgca
      301 tcctctgtcg gtgcgggtat tgaatatgaa tggaccggcc cctgcatagt cggcccggct
      361 aatgctccga ccacgattgc cgactgcccc ggcctttata ctcttacggt aaacaatacc
      421 ctcaacggct gctccgcctc cgataccgtc tctatattgc tggctttggc aacagccatc
      481 gcggttgtac ccgacacctt atatatatcg tgtgaaacag ggacggttgt gttggacggc
      541 agcagttcgg tgtatggcgt gtttgaatgg caattcaacg gagcgccctc ctccctttca
      601 gagatcagcc cggtggtgtc aatgcccggc gaatatgtgc ttttagtgaa taccctggca
      661 ctcaactgtc ccgatgcaga tacgaccatt gtgctcttgg attgccagat aagcgctgct
      721 attgctgcat taaccgacac gctgacctgc gcacaaacga cggccaccct caacggcagc
      781 gcgtcttccg caggccccgc tataacgtat agctggaccg gtccggctgc gggttgtatt
      841 gcatcggggc aaggtacctc tatggtgcag gtggtttgcc ccggcgaata caccctcacg
      901 gtgagcaaca cagcggtggg tatcagtgaa atggccactg tgatggtgca ggccgatacg
      961 ataagaccat tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc tgtattcggc
     1021 cttttgccct cctgaacggc agtgcttcat caaccggcgc cggaataatg tatgaatgga
     1081 ccgatgccgg aggagcattc gtcagcgcct tgccctcaga tacagcgacc gttccgggct
     1141 tgtattttct gtcggtcacc aatacggtca acggctgcca agcagtggat tttgttacca
     1201 tttccagaac cgcagaaccg ccggtcgtca atttcgggaa taccgttttt ccgtgcaacc
     1261 gggattcttt tttattgcgg gccttcccgg aacttgccgg attttcttat caaataacct
     1321 ggtccggccc cggcctgctg tccgacaacc atgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnngta catcatcgaa gtgctcaaca tggcaaccgg ctgcagcatt cgggattctg
     1441 tatgggtaac gcaacctgaa tgcggcccct gcatcgcagt atccccgccc gatacggtca
     1501 cttgtcaggt gacgtcggtt gtgttaaacg ctgcattttg cgaaccttgt ccgggctgta
     1561 cagtgcagtg gtcggtcatt gccggagcta ttttatcggg ggcgggtacg ctcatgccgg
     1621 aggtacgcgc aggcacatac acgttgtcgg ctactgatac cgctggagtg atcacaaccc
     1681 tgacggtgga agtacccgca gatacgctgc cgcctgctgc cgacgccggg ccggatcgaa
     1741 tcattacctg cgaggatgcg gtggttatgt tgggcgccgg ccctgttcct tcaccggaat
     1801 acaggtatcg ctggttgcaa agcaacgttt cacccggtca gaatcaattg ttggtgacgg
     1861 ttcaaacccc cggtttttac gttcttgagg tagaaaatat ggccaatggg tgtgttgcct
     1921 cggatacggt gttggtaaca atagacacct tgagacccgt tgcagaggcc ggcccggatc
     1981 aggtcatcac ctgcggcatg ccttttgcca ttcctgatgc agccggttcg acgctgggca
     2041 acaacattgc atacgcatgg tcaaccacat ctccgggtat catagctgcc ggggaaaaca
     2101 ccctgaaccc catcatcacc gcagcgggtt ggtatcgttt attagtaacc aatacgctca
     2161 atggctgcac tgccgaagac agccttcagg taacgctttc ggcgcaattg ccttttgtgc
     2221 ccgatgtgcc ggatatgacg ctcacctgct cggatacggt attgttgctc aacccgatat
     2281 tgccggatac agccggactg agttttcaat ggtgcagaat ggtgggtacg atgccaaccg
     2341 attgctccgt cgggcgtacc gtcagcatca acatgcccgg aacatggcga tttgaagtgt
     2401 tgaatacctc gacggggtgc cgaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnccc gttgcagagg ccggcgcttc gcctttaccg ctcaattgta cgcggctttc
     2521 cgttccgttg gcaggcatgg cggggcctgc aggagtgccg gtagaaattg catggagtac
     2581 ctccgggagc ggcacagcca tgcccgataa tgtgctgaat cctacggtga cttcgccggg
     2641 catttatacc ctcaccgtta ctcgtaccga caatggctgt tcggcctccg actcggtaga
     2701 ggtattgctc aacgataatt tcccggtggc caatgccggg ccggatacca gcctcacctg
     2761 ccgggttacg gagcttcggc ttcagggcag cgcccaaaca ataggcgccc ccgcgcaatg
     2821 gaactggagt agccccgatg ccacgattct ggaaggcgcc caaacgccca acccgcgcat
     2881 caaccaagcc gggctgtaca tactgaccat aacagacccg gccaatggct gtacggatac
     2941 cgacactgtt gtggtaatag ccaatcagct cccgcccgat attgacatcc aggcaccgga
     3001 aggctggacg ctcaattgtc tgcatccgac gctcgtattg gatgcgtcgg gcaccacctc
     3061 ttccggcccg gtgaggtttc aatggtttgg tacgccggga agtatagacg gccgggtgga
     3121 cagcagcgcc atccgcgcca acagcgtagg gttctatcgc ctcgttgtgg aagatgtgat
     3181 ccgcggttgc cgggatacgg ccattttgca aattggcggc gattttgcag tgccgtctgt
     3241 agctgttgcg gcccctcttg tcattacctg cgcccgcccg gaggtcacgc tgacagccat
     3301 accttctcct cccggcgatt tttataccac ccgctggctc ggcccttccg gcgataccgt
     3361 ggcaggagcg ggtttgactg cggctgtccg actcgctggc gcatggaggg tttgggtaac
     3421 cgataccagg aatggctgtt tcggcattgg caatatcatg gttgcagaag accttgtgcc
     3481 gcctgtggcg cgcatcgcgg cccctgatac gctggattgt accacgcggt cggtcttatt
     3541 aaatggctct gcctctgtag gtcggggtgc tttggcattt gagtggagtg gtccgtcggg
     3601 gggtattacc acgggaaata tggctgtgtc tgccaccgca atgctgccgg gcgtgtactt
     3661 tttggaagtc accgatgcac tgaatggctg tcgcgataca gcggaggtct tggtagtaga
     3721 aacttctgtc ccgatcacag gggtggaagc ggaaatcatt cccccggcct gccctggaac
     3781 gggagacggg agcatccact tgttgaatat tgagggaggc accggtcctt atggaattac
     3841 cttcaacggt agcggccctt ctcttactcg cgatttttac tatctcagcc ccggagttta
     3901 taccgtccga atacaggatg ccttgggatg tagtttagat acgagtttca acttgttgga
     3961 cccgccgtta ctgacagtgg atttgggcgc cgacctgttg attcggttgg gagacagcgt
     4021 caggttggaa gcgatggcca atttacctgt gacggcctat cgctgggcgc cggcagaagc
     4081 attttcagat gcaggtgctg ccgtacagtt ggtgacgcca ttgttcacaa caacctacca
     4141 ggtgacggta acgaatgccg aaggatgcac tgctacggat tggataacat tgacggtcaa
     4201 tactttgaat ctgtattttg caccagatgc cttctccccc aatggcgacg gcaacaatga
     4261 ctactatacc atatttgcag cgccggcgat taaggaaata cagtcgctcc atatttttga
     4321 ccgttgggga aacatggtgt tctcgcgcag caacttccct cccaacgacc cggcgctggg
     4381 ctgggatgga aactttgaag gcaggccgat gaatgcagcc gtgtttgtag tgatggccga
     4441 aattgtactg cccgatggaa ggctggagtt gtacaaaggc gagttgacgc tgcttcgtta
     4501 aggggaatag attaacagtt cgccccactc cttgtggcct tattgaataa acggcaaggt
     4561 aaagaaaatg gcgcttccca taccggcttc agactcggcc cagatttttc cgccgtgttt
     4621 ctccacgatt ttccggcaag tggccaatcc gatacccgaa ccttcgtagg catctcgcga
     4681 atgaagccgg gtaaacattt cgaacatttt cacccgaaac tcttccggga tgccgatgcc
     4741 attgtctttg acgtaaaaca gatactcttt tttatctggg tcgggcatgc agcccacctc
     4801 aacgacgggc gcttgttctc cccgaaactt gatgccgttg ctgatgaggt tttgaaacac
     4861 ctgccccagg cgcgagcggt tgcccataac ggccggcata gaagccacat ctaaatcaat
     4921 tcgggcgccg gaatcttgta tcacctgatg caggctgccg atgacttcat ttactacctc
     4981 ggttgtttct actttggcga agtcggtatt ttcccgttct atgcgggtgt atgccaacag
     5041 atcttttagc aacaactgaa gccgggctac gccattggtc acaaaatcca tgtattgctg
     5101 ggcctgctcg tccaattggt cggtatattt tcgtttgatg atgccgacga atgacccgat
     5161 ggtacgcagc ggttcctgaa ggtcgtggga tgctacataa gcgtattgcc tcaactcctc
     5221 attataagac tgtagcgtca ggttttggct ttcaatctgg ccgttgagtt cttgcagcac
     5281 cttattggat tcgcgttgcc ggcgtaccag tattaagacc caaatcaata gccctgctaa
     5341 ggctgccaaa aaaacagaca gacctatcat ggtgcgctgg ttgcctttcc gtctgatttc
     5401 ggccagttct ttttcctgct gcaacacctt tatggcgttt tcttttttac ccacttcgta
     5461 cagggtgttg agcttgataa tttgattgtt ggaagactca ttgatcagag agtctttcat
     5521 catgatgtac tctctcaggt aagcattgga ggcttcgtag ttgcctttca attgctgcaa
     5581 ctgagccagt cgctccagta cttccattaa aatagaattc gatgcggact ctatgcataa
     5641 taaatgagcc tggcgcaatg atttttccgc atcagcgtaa gtattggtct gcatttgtaa
     5701 ctcgcccatg cgcaaatgac ataaaataga aagccggtgg ttgtcgagcg ctgtactcgt
     5761 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcggagcga ttcttgttga tcgcccaact
     5941 cctcatacgc gctgccaacg gctgagaggc aattcagcaa atttttgcgg ggtatggaga
     6001 tgtctttttc acaaagatgg agggcttttt tatagtattc taatgactta tcgtactttt
     6061 cctgtgaaaa atacatagat ccgatcttat aaagggactc tgccgtacct tcggcattgc
     6121 cggctttttc gcgattttcc aaggctttga gttgatactc caaggctttg tcgtagttac
     6181 ccaaggcttc gtaaatcgct ccgagaaggc tcaatacttt agcttgttct tcgggtttat
     6241 tattgggcag aagcgggttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnncgcagca ttatattctg agagctttac cagcgtttga gccagtttaa ttttggcgct
     6361 gagggcggcg gattcattgc gctgtttttc agactctttg agcgcttgcg cataataacc
     6421 ttgcgctttt ttgttctcat caatgcttag taaactatcg ccgattgcca ttaggtatgg
     6481 aactccgtca ctgggccggg gagaggctgc atcggcttgc gcatacgagt tttggccaga
     6541 tacgagagtc agtatgccaa aaactaatga agtgcagatt ctgaatagac aaatattgac
     6601 ggacatacta attgagcttt gggagttaag tataaaaaaa tgccgctctg ccgggcataa
     6661 agaccaacag agcgacaaaa atactttttt cgaatgattc agggcaatta atcccagtca
     6721 ggcggattgc ctttgacgtc ccagtctggt gggttgccgt gcaaatccca atctggtggg
     6781 ttgccgtgca aatcccaatc aggcggattg ccgtgtaagt cccaatctgg tggattgcct
     6841 tgagcaccgc ccttaatgga ctgttgctcc tccttggaaa gcggcttcat ctcagcctgc
     6901 tttttgttgc gcttttgaaa gttgaacata atttggaggg ttaaaatatg agaaaaataa
     6961 aaaccaacat ccaattgact aagcctctca taagaaagac gacagctgca ttgaaatgtt
     7021 gttcgtagtg ttcgactttt gaaatagaat gtgcatcaaa aaaggggatg acgcagtatg
     7081 aatatcgttt tctcacgcat tgtcgccaca aagatataac aaaccctccg aaaaatcaag
     7141 caataaacaa aaaaaggggg aacaaaatgc tcccccctaa aaaacaccta aaacccatat
     7201 tctgtcatga aaacattatc attatatgcc tttattctcg ccattgtgac gaaaacgggc
     7261 gcaaatgtca caaaaaaaga ggaaaatcaa aaaattttcc tctaaaaaac acctaaaacc
     7321 catattctgt catgaaaata atctctttgt aaaagatgac tttgagacgg agcacccaga
     7381 cataggtcac aaaaaaagga gaaccgtaag attctcctct caaaaaacac ctaaaaccca
     7441 tattcatatc atgaaaacct tatgccatgt cggcctttca tcggttgatg tctcggccgt
     7501 ttgttggtgc aaagatacat gcgccggaat ctgccaaaaa gtgacaatca tcacaataag
     7561 gcctgttgtt ttgctttttt gcgcatcttt gcggccttaa actgcaagca tgtcgaaaac
     7621 cctttctatt atcatccctg cttacaatga ggcacgcacg attcgattca ttttggataa
     7681 agtgcgaatg gcgaccttgc cccaagaaat aggcagagaa gttatcattg tcaatgactg
     7741 ttctaccgat gatacccgtg aagccattca gcagtatatg gccgagtatc ccgacatggg
     7801 gattcagtac agagagcacc gcgtcaatca ggggaaaggc gccgctttgc acaccggcat
     7861 ccgggaggca tccggagatt acatcatcat acaggatgca gatttggagt acgacccgcg
     7921 cgaatatact ttactgttga tgccgatact ggaaggtatg gccgacgtgg tatatggatc
     7981 gcgcttcatg ggaggcaacc cgcaccgcat ccttttcttt tggcattcta tcggcaataa
     8041 gtttcttact tccttgtcca atgctatgac caacctcaat ctgaccgaca tggaaacctg
     8101 ctacaaactg tttcgggcag acatcgtcaa aagccttgtt ctgaaagaaa agagattcgg
     8161 cttcgagccg gaagtgacgg ctaaagtagc cagggtgccg ggcgtgcgca tttacgaggt
     8221 ggggatatcc tactacgggc gcacttatgc cgagggtaag aagatcaatt ggaaggacgg
     8281 attcagggcc atttactgta tcctgaaata taacatttgg ggttgaatga aatttgagtg
     8341 aaaaaataat ttcaaaacgc gtatatttgc gcggtttttc cggggcggca cattttgccc
     8401 tgaaaatcag gagcttcggc tcgcgtttta taatccttaa aatgaatcgt gtaccatgca
     8461 aggtaaagga gtagttcggt ttttcctcat tgttgtagcg ctggtgaccc tggcgcagtt
     8521 cctgctcatc attccgacga ggcgggtaga aaaaaatgca gacgcttatg caaccgctgc
     8581 gtcggccaat gcgccggcag aaagcaagac ggctgttttt aaagaaaagc gcatcgctta
     8641 tttagactcg ctctcctcag agccggtgtt tacggtgcct ttgatcaagt cttacaccta
     8701 tcaagacctc aagcgccagc aaattgcgct tggcctcgac ctcaaaggcg gtatgagcgt
     8761 ggtgttgcag gtggacttgc gcaagtttgt caacgccttg gccaacgaga gcagcgacgc
     8821 tgactttaaa gctgctttgg atgccgcttt ggatgctcag aaaaactctg aagcagactt
     8881 cattacgctg tttgccgata acttccgcag catcagtaac ggcaagccgc tggcgcccat
     8941 ttttgcccgc aacgaagcgc tggggctcaa tttcgagtct tccgacggcg atgtcgtgca
     9001 ggtacttcgt gcgcgcgccg acgaaacggt ggggcttacc ttcgaccgtc tcaaagagcg
     9061 cattgacaaa ctcggcgtag tgcagcccaa catttctttg gatgccgccc gcgatattat
     9121 cgtggtagaa ttgcctggta ttgacaaccc ggaacgcgcc cgtcgctttt tgcaggctgc
     9181 cgccgaactc gaattctgga atgtatatcg catcagcgat ccgggtatga tggaggcatt
     9241 tgctgccgcc aatgagcgcc tggccaaaat gaaaggcacg gaggcctcta cgcagcgcca
     9301 gattttgaaa atagacacct tatataaggt ggacgaggcc ggcaattcgg ttgtagatcg
     9361 tttggatact acctatgcgc ccatcaccgg cgggccgctg tttgatgtgt ttaccatcaa
     9421 cagttccggt tcttatggct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactac tcaaaaagct accgataatt
     9601 acgaactgta cgccatccgc aaggagcgcg gcggcgacaa tgcaccgctg accggtgaag
     9661 tcgttacgaa cgcatcggca cagcccgatg agaacaacca aatggcggta tcgctgcaaa
     9721 tgaatgccag cggcgcccaa atctggggtc agatgactac gaaggctgcg caggacaaca
     9781 accgcgaggt agccattatg ctcgacagcg cggtcgtttc cgctcc
//
LOCUS       DAORLF010000141         9817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3708_length_9817_cov_2.103258, whole genome shotgun sequence.
ACCESSION   DAORLF010000141 DAORLF010000000
VERSION     DAORLF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3708_length_9817_cov_2.103258"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK198_12380"
     CDS             complement(<1..262)
                     /locus_tag="PK198_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF39576.1"
                     /translation="MTEQTLIQACIRQERKAQKMLYEQYAPLVMGICRRYLKSEEDAE
                     DVLVETMYKVLSKLPEFKSEGSFEGWVRRIAVNECLMSLRKNQ"
     assembly_gap    335..353
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            388..>719
                     /locus_tag="PK198_12385"
     CDS             388..>719
                     /locus_tag="PK198_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRF39577.1"
                     /translation="MESPSNRLLLSGNIDPLDLVQQFGCPLYVYDTAVMERQYRRMAN
                     AFSVKRLRINYAVKALSNINVIKLFRSLGSGLDTVSIQEVMLGLKAGFAPRDITFTPN
                     CVSLDELL"
     assembly_gap    720..736
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <737..>1373
                     /locus_tag="PK198_12390"
     CDS             <737..>1373
                     /locus_tag="PK198_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRF39578.1"
                     /translation="GVRINIDNISILEQFGQARPDVPVCIRINPHIMAGGNLKTSVGH
                     IDSKFGISIHMIPMVHRVIEATGLRVEGIHMHTGSDILDPDVFVAGADVLLKYAGEFP
                     DLDYVDFGSGFKVPYKPGDIETDIEELGEKISERFNEFCAEYGRDLEMVFEPGKFLVS
                     AAGYFFVKVNVIKYTTSTVFAGVDSGLNHLLRPMFYNAYHHITNVSNPDAKP"
     assembly_gap    1374..1383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1384..1631
                     /locus_tag="PK198_12395"
     CDS             <1384..1631
                     /locus_tag="PK198_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRF39579.1"
                     /translation="YAIVGYICETDTFGINRRISEIMEGDILCIHNAGAYGFVMSSNY
                     NSRYRPAEVLIHEGKAHLVRRRETMEDILRNQIEVEI"
     gene            complement(1610..2776)
                     /locus_tag="PK198_12400"
     CDS             complement(1610..2776)
                     /locus_tag="PK198_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39580.1"
                     /translation="MEYLKRSIEPLIKEYLLPNKVLVLLGPRRVGKTVLLRQILSEIT
                     EPFLLLNGEDINTKELFNRRSLPHFLQLLDGKKLLIIDEAQKIPDIGHALKLMIDEIV
                     GLRILITGSSAFDVENFTGEPLTGRKFTFKLFALSEAEYDQRDSILIRKDKLRERLVY
                     GNYPELLQLQTTEQKNRYLNDLVSSYLLKDILTFESIKNSDKILALLRLLAFQTGSEV
                     STTEIGKQLSMSSNTVSRYLDLLSKVFIIHRVGGFSRNLRKEISKSSKWYFFDNGIRN
                     ILVANLNPIDLRNDAGQLWENYIISERIKFQQNTGMIVNNYFWRTYDQQEIDWIEERG
                     GDLHGYEFKWNEKKQPKAPGGWKEGYPDASYQVLTPENYADWVRNDLPYISTSI"
     gene            complement(2915..3277)
                     /locus_tag="PK198_12405"
     CDS             complement(2915..3277)
                     /locus_tag="PK198_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39581.1"
                     /translation="MSLLASRFDTLNYLAKSLAEGHSFEIDARAFADLVLQLRDDLYD
                     YTSSPAILDRVDQIPDLDLAPHQRSFLEQLLPSGARSMVGDYQVKQKILAQVNDITRA
                     FNRIRDMLPIGPSEEDFV"
     gene            3470..>4257
                     /locus_tag="PK198_12410"
     CDS             3470..>4257
                     /locus_tag="PK198_12410"
                     /inference="COORDINATES: protein motif:HMM:NF037184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39582.1"
                     /translation="MILFFGNPLLKIFTVQLDPKLQTALNADDLQKLSWLFGNEPLIK
                     AERVPGPFVGPRVAMISPWSTNAVEITQNMAIQGIVRMEEFFKIEESGHYDPMLSQKF
                     AELNQDIFTIHIEPAPILEIDNIAAFNEQEGLALSAEEIAYLDGLAQRIGRKLTDSEV
                     FGFSQVNSEHCRHKIFNGAFVIDGEEKPRSLFQLIKKTSAENPNDIVSAYKDNVAFLK
                     GPVLSQFAPKRPDRPDFYEKKRFESVLSLKAETHNFPTTVEPFNG"
     assembly_gap    4258..4334
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4335..>5752
                     /locus_tag="PK198_12415"
     CDS             <4335..>5752
                     /locus_tag="PK198_12415"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRF39583.1"
                     /translation="GTAVYMTAYSRLLDNRPWEKGMPERKWLYQTPLDILIKASNGAS
                     DFGNKFGQPLIAGSLFTFEHEEAGRQLGYDKVIMLAGGIGYGKAAQALKGTPETGDKI
                     VVMGGDNYRIGMGGAAVSSADTGEFNTAITLNAVQRSNPEMQKRVANAIRGVVESDEN
                     FIVSIHDHGAGGHLNCLSELVEATGGSIDLDQLPVGDPTLSAKEIIGNESQERMGLVV
                     LEKHLETLRHIAERERAPMYEVGDVTGDLRFSFRSASKGSRPMDLALSDMFGSSPKVV
                     MTDTHRRTQYAGVDSHASQLQTYIRDVLRLEAVASKDWLTNKVDRCVGGLVAKQQCAG
                     PLQVPLNNCGVMALDFDGKSGLATSIGHSPISGLIDPVAGSRNSVAEALTNIVWAPLE
                     KGLRSVSLSANWMWPCKNPGEDARLYEAVQAISDFAIALGINIPTGKDSLSMKQKYPD
                     GHEVISPGTVIVSAAGHCNDRY"
     assembly_gap    5753..5764
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5765..7158
                     /locus_tag="PK198_12420"
     CDS             <5765..7158
                     /locus_tag="PK198_12420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRF39584.1"
                     /translation="LQKTDGPIYYLNLSGDDFQLGGSSFAQTRNSLGDQAPTVNDAAY
                     FAKAFDALQNLIKSGLVLAGHDVSSGGLLTCLLEMCFADLDLGAELDLTPMGESDSVK
                     LLFAENAGVVIQAQNDSVEKLLNEAGISFFKIGKATSSAVLSVKNGDETHTFDVAELR
                     DVWYETSYLLDSHQTAGELAKTRFDNYKTQPLHYNFPQGFDGKRPVRDESKPRIKAAI
                     IREKGSNSEREMAHAMYLAGFDVKDVHTTDLISGRETLEDVRFIGAVGGFSNSDVLGS
                     AKGWAGALLYNEKARQALDNFFRRDDVLSVGICNGCQLFVELNLIHPEHATPPRMLHN
                     DSHKHESIFTSVVIPKNNSVMLRSLAGCRLGIWVSHGEGKFHLPLPESAYNIVAKYGY
                     DAYPANPNGSDYNAAMICSADGRHLATMPHFERSMFRWNWAHYPADRQSDDISPWVEA
                     FLNARRWLEQGEQ"
     gene            complement(7223..7681)
                     /locus_tag="PK198_12425"
     CDS             complement(7223..7681)
                     /locus_tag="PK198_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39585.1"
                     /translation="MKKLQFKKEINAIAQKVYETMLGLKDKSTYEHWTSVFNPTSTYE
                     GSWEKGSKIHFVGMDENGKKGGMVSEIEEHQPAQFVSIRHYGFLDGDIEITTGEQVEK
                     WAGGHENYSYHEDNGVTTVTVDLDTVDEYLDYFESTYPAALDKLKEMLER"
     gene            7972..>9406
                     /locus_tag="PK198_12430"
     CDS             7972..>9406
                     /locus_tag="PK198_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF39586.1"
                     /translation="MTLPEKNITEIYEKEITQELRINFKTYQTNPSDAFIQSKIRKDI
                     FKIMDMNMQAIQRKSELAKRTADSAVLGIFITGTLCFLIAFVLLVNLPANIADPIKEL
                     TQSIQQIATKNYAERVHFESHSEFGQLAKSFNAMAENLEEYNNSSLAKLMMEKKRIET
                     LIDKMHDPVIGLDENMLIIFANEEATNILGLPYSELIGFPSKDLALKNDLLRELIKDV
                     HPVDEAAVSIKPIKIYANDKESYFERDVQHITIIPTGELRPKTIGHVILLRNVTEYKE
                     LDFAKTNFIATISHEFKTPISSIKMSLQLLENEQIGKLNEEQKNLLESIKDDATRLLK
                     ITGELLNMTQVESGNIQLTILPVNPKDILQYAINATKTQAEQKQIIFEIDCPDSIPPI
                     LADSEKTAWVITNLISNAIRYSYDHSSVYLMIRETENQVAISVKDIGQGIAPQYKDKI
                     FDRYFRVPGTQKEGTGLGLAISKEVIEA"
     assembly_gap    9407..9525
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9591..>9817)
                     /locus_tag="PK198_12435"
     CDS             complement(9591..>9817)
                     /locus_tag="PK198_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39587.1"
                     /translation="PQHHCGYVFLLVQTTHLLPENVYFISTPFLAHTKLSQKPEKGRL
                     KFSGFVFGLVQNRPQTCGKACGKVIRGVGD"
BASE COUNT         2429 a         2546 c         2253 g         2335 t
ORIGIN      
        1 gttgattttt tcgcaggctc atcaggcatt cgttgacggc aatgcgccgc acccagccct
       61 cgaaactacc ttcgcttttg aactccggca acttcgacaa caccttatac atcgtttcta
      121 ccagcacgtc ctcggcgtct tcctcacttt tgaggtagcg tctgcaaata cccattacca
      181 aaggcgcgta ctgctcatac agcatcttct gggcctttcg ctcctgcctg atacaggctt
      241 gtatgagtgt ttgctcggtc acgcgtcttt tgtttccgac gtaaagatgc aagccgcgcc
      301 gaaattggcg tatgggcagg caggcttttt tttcnnnnnn nnnnnnnnnn nnngcatacc
      361 tttgcgcccc tgattaatca aaacaacatg gaatcaccct ccaaccgcct gctcctgtcg
      421 ggcaatattg atccactcga tttggtacaa caattcggct gcccgctgta tgtgtatgac
      481 accgccgtga tggagcgcca gtatcgccgc atggccaatg cgttcagtgt gaaacgactc
      541 cgcatcaact atgccgtcaa agcgctcagc aacatcaacg tgatcaagct attccgcagc
      601 cttggctccg gcttggacac tgtgtccatt caggaagtca tgctgggcct caaggccggt
      661 tttgcccccc gcgacatcac gtttacgccc aattgtgtga gcttggacga gctgctggan
      721 nnnnnnnnnn nnnnnncggc gtacgcatca atattgacaa tatttccatc ctcgaacaat
      781 ttggccaggc gcgccccgat gtgccggtgt gtatccgcat caacccgcac atcatggcgg
      841 gcggcaacct caaaaccagc gtggggcata tagattccaa gtttggcatt tccatccaca
      901 tgattcctat ggtgcatcgc gtcatcgagg ctacgggctt gcgcgtggaa ggcattcaca
      961 tgcacaccgg ttcggacatt ctcgaccccg atgtatttgt agccggtgcc gatgtgttgc
     1021 tcaaatatgc cggcgagttt cccgatctgg attatgtgga tttcgggagc gggttcaaag
     1081 tgccgtacaa acccggcgat atcgagaccg acatagagga gttgggcgaa aagatttcgg
     1141 agcgcttcaa tgaattttgc gccgagtatg gccgcgatct ggagatggtg ttcgagccgg
     1201 gcaagttttt ggtgagcgcc gcgggctact tttttgtcaa agtcaacgtc atcaaataca
     1261 cgacttcaac ggtgtttgcc ggcgtggatt cggggctgaa ccacctgctg cggcctatgt
     1321 tttacaatgc ctaccaccac atcaccaacg tatcgaaccc cgacgccaag ccgnnnnnnn
     1381 nnntttacgc tatcgtgggc tacatctgcg aaacggatac attcggtatc aaccgccgca
     1441 tttccgaaat catggagggc gatatcctct gcatacacaa tgcgggtgct tatggctttg
     1501 tcatgtcgtc caactacaat tcccgctacc gtcctgccga agtgctcatc catgagggaa
     1561 aagcgcattt ggttcgccgc cgggaaacga tggaggatat tttgagaaat cagattgagg
     1621 tggaaatata aggcaagtcg tttctcaccc aatcggcata attttccggc gtcaatactt
     1681 gatagcttgc gtcaggatag ccctccttcc agccgccagg cgctttgggt tgttttttct
     1741 cattccattt aaactcatac ccgtgcaagt cgccaccccg ttcttctatc caatcaattt
     1801 cctgttggtc gtaagtgcgc cagaagtaat tattgacgat catgcccgta ttttgctgaa
     1861 acttgattcg ctcgctgatg atgtagtttt cccatagttg gccggcatca tttctgagat
     1921 ctatcggatt tagattggcc actaaaatgt tcctgatgcc attgtcgaaa aaataccact
     1981 tgctgctttt ggaaatttct tttcgcaggt tcctgctgaa gccccccacc ctgtgtatga
     2041 tgaaaacttt ggataacagg tctaagtacc tgcttaccgt attgctgctc atactcagct
     2101 gtttgccgat ttcggtggta gaaacctcgc ttccggtctg aaaagccagg agacgcaaca
     2161 aggccagtat tttgtccgaa tttttgatgc tttcaaatgt caggatgtcc ttcagtaaat
     2221 atgaactcac cagatcgttc aggtatctgt ttttttgttc ggtagtttgt aattggagca
     2281 gttctggata attgccatat actaaacgtt ctctcaattt gtcttttctg atcagaatcg
     2341 aatctcgttg atcgtactct gcctccgaga gcgcaaagag tttgaatgta aatttcctgc
     2401 cggtaagagg ttcgccggta aagttttcca catcgaaggc ggaggagccg gtaatcaaaa
     2461 tcctaagtcc cactatttcg tcaatcatta gtttcagggc atgtccgatg tccggtatct
     2521 tctgcgcctc gtctatgatc aataattttt ttccatccaa cagttgtaaa aaatggggca
     2581 ggctccttct gttgaacaac tctttcgtgt tgatatcttc accattcaac agcaggaaag
     2641 gctctgttat ctctgacaaa atctggcgaa gcaggacggt tttacccact cgtctcggcc
     2701 ccagcaacac caacactttg ttgggcaaaa ggtattcttt tatcagcggc tcgatgcttc
     2761 tcttaagata ttccatcgtt cgtttttgtg caaaagtacg gaaatttaaa ccatcaactg
     2821 agctaattaa cgttaattaa ctctattcac tgagctaatt aacgttaatt ggctcagtat
     2881 aaggcgtgct ttcggaatat gaagtgaccc tctgctacac aaaatcctcc tccgaaggcc
     2941 ctatcggcag catgtcccgg atgcgattga aagcgcgggt gatgtcgttg acctgagcta
     3001 atattttctg ttttacctga taatcgccca ccatgctgcg cgcaccggaa ggcaggagtt
     3061 gttccagaaa cgagcgctga tggggcgcca gatcgaggtc gggaatctga tctacccggt
     3121 ctaagatggc cggcgacgag gtgtagtcgt agaggtcgtc gcgcagttgc agcacgagat
     3181 cggcaaaggc gcgggcatca atctcaaaac tgtggccctc ggccaggctt ttggccaaat
     3241 agttgagcgt atcgaaacgg gaagcgagca ggctcatggc gtggtgtttt tatctgtctg
     3301 caaacttagg cggattccgt caaatgcctt gcgattattt cgaccagaca tgtactgtgg
     3361 actaaacacg aagatagaat ctcagcacaa gccgaatcga aattggaatt tagtgccggt
     3421 atgcgctacc tttgccgcct caacctttca cagcaaacag cgcgccacca tgatcctgtt
     3481 tttcggcaac ccgctcctca aaatcttcac cgttcagctc gacccaaagt tgcagacagc
     3541 tttgaacgcc gacgacctcc aaaaactcag ctggctcttc ggcaacgaac cgctcataaa
     3601 agcggagcgc gtgcccggcc ctttcgtcgg acctcgcgtc gccatgatct cgccctggag
     3661 caccaacgca gtggaaatca cccaaaacat ggccatccag ggcatcgtgc gcatggagga
     3721 gtttttcaaa atagaggaaa gcggccatta cgaccccatg ctttcgcaga agtttgcgga
     3781 gctgaaccag gacattttca ccatccacat cgaaccggcg cccatcctcg aaatagacaa
     3841 catcgcggct ttcaacgagc aggaaggact ggccctgagc gccgaagaaa tcgcctacct
     3901 cgacggcctc gcgcaacgca tcgggcgcaa actcaccgac tcggaggtct tcggcttctc
     3961 gcaggtaaac agcgagcatt gccgccacaa gattttcaac ggcgcctttg tgattgacgg
     4021 cgaggaaaaa ccgcgctccc ttttccaact catcaaaaag acctccgcag aaaatcccaa
     4081 cgacatcgtt tctgcttaca aagacaatgt ggcatttttg aaaggtcccg tcctttcgca
     4141 gtttgctccc aagcgccccg acaggcctga tttttatgaa aaaaagcgct tcgaatcggt
     4201 gctctcgctg aaggccgaaa cgcacaattt tcccaccacc gtagagccgt tcaacggnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnngggcac ggcggtctat atgacggcct attcgcgact tttggacaat
     4381 cgcccctggg aaaagggcat gcccgaaaga aaatggctgt accaaacccc gctcgatatt
     4441 ttgatcaaag ccagcaacgg cgcctccgat ttcggcaata aattcgggca accgctcatc
     4501 gccggctcgc tgttcacctt cgaacacgag gaagcgggac ggcaactcgg ctacgacaaa
     4561 gtcatcatgc tggcgggcgg catcggctac ggcaaggcgg cgcaggcact caagggcacg
     4621 cccgaaaccg gcgataaaat cgtggtgatg ggcggcgaca actaccgcat cggtatgggc
     4681 ggagcggccg tttcctcagc agatacaggc gaattcaaca ccgccatcac gctcaatgca
     4741 gtacagcgct ccaaccccga aatgcaaaaa cgggtagcca acgccattcg gggcgttgtg
     4801 gaaagcgacg agaatttcat cgtctccatc cacgatcacg gggcgggcgg ccacctcaac
     4861 tgcctctccg aattggtgga agccaccggc ggcagcattg acctcgacca actgcccgtt
     4921 ggcgacccca cgctgtcggc caaagaaatc atcggcaacg aatcacagga gcgcatgggc
     4981 ttggtggttt tagaaaaaca cttggaaacg ctgcgccaca tcgccgagcg ggagcgcgcc
     5041 ccgatgtatg aagtcggcga tgtgacgggc gacctgcgct tcagcttccg atcggcctcc
     5101 aaaggcagcc gtccgatgga cctggcactg tccgatatgt tcggcagttc gcccaaggtc
     5161 gttatgacag atacccaccg gcgcacccaa tatgccggtg tggacagcca cgcctcgcaa
     5221 ctgcaaactt acatccggga cgtgctgcgc ctcgaagccg tggccagcaa agactggctg
     5281 accaacaagg tggaccgctg tgtcggcggc ctcgtggcca agcagcaatg cgccggtccg
     5341 ctccaagtcc ccctcaacaa ctgcggcgtg atggccctcg acttcgacgg caaaagcggc
     5401 ctcgccacct ccatcggcca cagccccatc agcggcctga ttgaccccgt agcgggcagt
     5461 cgcaactccg tagccgaagc gctgaccaac atcgtgtggg cgcctttgga aaaaggcctg
     5521 cgctccgttt cgctttccgc caactggatg tggccctgca aaaaccccgg cgaggatgcc
     5581 cgcctgtacg aagcagtgca ggccatttca gactttgcca ttgcgctggg catcaacatc
     5641 ccgacgggca aggactcgct ctcgatgaag caaaaatacc ccgacggcca cgaggtcatt
     5701 tcgcccggca cggtcatcgt gtcggcggcg ggccattgca acgaccgcta cgnnnnnnnn
     5761 nnnnttttgc aaaaaacgga cgggccgata tactacctca atctctccgg cgacgacttc
     5821 caactcggcg gctccagctt tgcccaaacc agaaacagct taggcgatca agctccgacc
     5881 gtcaatgatg cggcatattt tgcaaaggct tttgacgccc tgcaaaacct gatcaaaagc
     5941 ggtcttgtac tggccggaca cgatgtttcc tccggtgggc ttttgacctg tttgttggaa
     6001 atgtgttttg ccgacctcga tctgggagcg gagttggatt tgaccccgat gggcgagtct
     6061 gattcggtga aattgctgtt tgcagaaaat gccggcgtgg tgatacaggc ccaaaatgat
     6121 tccgtggaaa aactgctgaa cgaggctgga atttcctttt tcaaaatcgg gaaagccacg
     6181 tccagtgcag tgttatccgt gaaaaacggc gatgaaacgc acactttcga tgtcgccgaa
     6241 ttgcgggatg tctggtacga aacctcctac ctcctcgaca gccatcagac tgccggcgaa
     6301 ttagccaaaa ctcgttttga caactacaaa acacagccgc tgcactacaa tttccctcaa
     6361 ggttttgatg gaaaacgccc cgtacgagac gagtcgaagc cccgcatcaa agcagccatc
     6421 atccgcgaaa agggcagcaa ttcggagcgg gaaatggcgc acgccatgta cttggccggc
     6481 ttcgatgtga aggacgtgca taccaccgat ctcatttccg ggcgggaaac gctggaagat
     6541 gtgcgcttta tcggcgctgt gggtggtttc tccaattccg acgtgctcgg ctcggccaag
     6601 ggctgggcgg gcgcattgct ctataatgaa aaagcgcggc aggcgcttga caatttcttc
     6661 cggcgggacg acgtgctctc cgtcggcatt tgcaacggct gtcaactctt cgtagaactg
     6721 aatctcatcc atcccgaaca cgccacgccg ccccgaatgt tgcacaacga ttcgcacaaa
     6781 cacgagagca ttttcacctc tgtggtcatt cccaaaaaca attccgtgat gctgcgctcg
     6841 ctggcgggtt gccgcctcgg catctgggtg agccacggag agggcaaatt ccacctgccc
     6901 ctgccggaaa gcgcgtacaa cattgtggcc aaatacggct acgacgccta ccccgccaac
     6961 ccgaacggca gcgactacaa cgccgctatg atttgcagcg ccgacgggcg gcacctagcc
     7021 accatgccgc acttcgagcg ctcgatgttc cgctggaatt gggcgcacta ccccgccgac
     7081 cggcaaagtg acgatatttc gccctgggtg gaggcgtttt tgaatgcaag gaggtggttg
     7141 gagcaagggg aacaataaag ccgcctcctt tgaaaaatgg tttatatttt tcgcaaacca
     7201 tttttcatcg ggtatcaagt aactatcgct ccaacatttc ttttaattta tctaatgctg
     7261 ccggatacgt gctttcaaaa taatccagat attcatcaac cgtatccaaa tctacagtta
     7321 cagttgtcac accgttgtct tcatgatatg aataattttc gtgtccgccc gcccattttt
     7381 cgacttgttc gccggttgtt atttcgatat cgccatccaa aaagccgtaa tgccttatgg
     7441 atacaaattg ggcaggttgg tgttcttcga tttcagaaac cattccgccc tttttgccat
     7501 tttcgtccat gcccacaaaa tgaattttac ttcctttttc ccaacttcct tcgtaagtag
     7561 aagtaggatt gaacacggat gtccaatgct cataggtgct tttgtctttc aagcccagca
     7621 ttgtttcgta aactttttga gctattgcgt ttatctcttt tttgaattgt agttttttca
     7681 ttttattgtc gttatttaaa agtcaaaatt tcacttattt tctgtcagcg gtatcgcgct
     7741 aaacggacta ccactgatca gaaagcacaa aaagtgtacc accggaatca aagttattac
     7801 aagttattga aatggattaa aaaatcttta atgcggcaac catcgcctgt cgctgggagt
     7861 cctccgcaca acacctctgc ggataggacg tttatccccc acctgccgac gtgtttactg
     7921 ttgtgttctg aatgttgcat gtagttttat tttggtagtg tcactcgtta tatgacacta
     7981 ccagaaaaaa atataactga aatatatgaa aaagaaatca cccaggaact aagaatcaat
     8041 tttaaaacct atcaaaccaa tccatctgac gcgtttatcc aatcaaaaat ccggaaagat
     8101 atttttaaaa taatggatat gaatatgcag gccattcaac gcaagagtga actggcgaaa
     8161 agaacagccg atagcgccgt gttgggaatc ttcatcaccg gaacactttg tttcctcatt
     8221 gcattcgttt tattggttaa tctgcctgcc aatattgccg atccaattaa agagctgacc
     8281 caaagcattc aacagatcgc cacaaaaaat tatgcggaac gtgttcattt tgaaagtcac
     8341 agcgaatttg ggcaattggc aaaatccttt aacgccatgg ctgaaaacct cgaagaatac
     8401 aacaacagca gcttggccaa actgatgatg gaaaaaaaga gaatagagac actgattgat
     8461 aaaatgcacg atcccgtgat aggtctggat gaaaatatgt tgatcatttt tgccaacgaa
     8521 gaagctacca atattttagg gcttccatat tccgaactca ttgggttccc ttcaaaagat
     8581 ttagccttaa aaaatgattt attgcgtgaa ctgattaaag acgttcatcc agttgatgag
     8641 gctgctgtct ccataaagcc cataaaaata tacgcaaatg ataaagaaag ttactttgaa
     8701 agagatgttc agcacattac catcattccc acaggcgaat tgagacctaa gaccatcggg
     8761 cacgttattt tactccgcaa tgtaaccgaa tacaaagagt tggatttcgc aaaaacaaac
     8821 ttcatagcta ccatttctca cgaatttaaa accccgatct cctccatcaa aatgagctta
     8881 caactattgg aaaatgagca aataggcaag ctcaacgagg agcaaaaaaa tctgctcgaa
     8941 agcatcaagg acgatgcaac caggctgctg aaaatcacag gggaactact gaatatgaca
     9001 caggtagaaa gtggcaatat tcagcttacc attctacctg tcaatccgaa agatattttg
     9061 caatatgcca tcaatgccac aaaaactcag gccgaacaaa aacaaataat ttttgaaatt
     9121 gattgtcccg attccatacc cccaatactt gccgacagtg agaaaacagc ttgggttatc
     9181 accaatttaa tttccaatgc cattcgctac tcttacgatc attcttcggt ttatttgatg
     9241 atccgggaaa cagaaaatca agttgcgatc tccgttaaag atattgggca aggtattgcg
     9301 ccacaataca aagacaaaat tttcgaccgc tattttcgcg ttccgggaac ccagaaagaa
     9361 ggcacaggac tcggcttggc catcagtaaa gaggttattg aagcacnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatta ttctacttcc
     9541 cgctaccatt tcaaacctct tcgcaatgaa tcacttgagc cggcatgccc tcaatcccca
     9601 acaccccgta tcacttttcc acaggctttt ccacaggttt gcgggcggtt ttgcacaagg
     9661 ccgaaaacaa accccgaaaa tttcaaacgt cccttctcag gtttttggct tagctttgtg
     9721 tgagcaagga acggggtcga aataaagtac acattttcag ggagcagatg ggtcgtctga
     9781 acgagcagaa aaacgtagcc gcagtggtgc tgcggcg
//
LOCUS       DAORLF010000142         9803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3712_length_9803_cov_2.134694, whole genome shotgun sequence.
ACCESSION   DAORLF010000142 DAORLF010000000
VERSION     DAORLF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_9803_cov_2.134694"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1037)
                     /gene="ygiD"
                     /locus_tag="PK198_12440"
     CDS             complement(153..1037)
                     /gene="ygiD"
                     /locus_tag="PK198_12440"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRF39588.1"
                     /translation="MERKTFLKWLALLPATGILMKLRTFGDSVSELPSTGLMPVLFLG
                     HGSPMNAIETNEFSQNWRKIGESIPRPKAILCVSAHWETRGTWVTAMQQPRTIHDFGG
                     FPQALYDVQYPAPGSPEMALETQKTITSTQVGMDQQWGLDHGCWSVVKHLYPNADVPV
                     FQLSLDRFQTPQYHYELGRELAALRRKGVLIVGSGNMVHNLRMVAWDKLHLPEYGYDW
                     AIEANESMKKLIATGDHEALTAFRNRGKAFDLAIPTPDHYLPLLYVLAGKQGDEPVRI
                     FNDRVIGGSLNMTSVQIG"
     assembly_gap    1108..1154
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1238..2359
                     /locus_tag="PK198_12445"
     CDS             1238..2359
                     /locus_tag="PK198_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF39589.1"
                     /translation="MKRVHLFFLLLIFWAVWTASGCNPDDPDPPKDELISGEYAPQSV
                     TLTVPNWMPQPLIPADNPLTEEGIELGRRLFYDPILSSDSTQSCASCHRQEKAFTDGL
                     AVSTGVRGLPGRRSAMALDNLSFHAREFFWDGRSPSLEAQALVPVEDPLEMNETWPNV
                     LEKLRRHPDYPRRFRAAFGIEKKSEITKELAAKAIAQFERTLVSANSRYDRVIWLNDG
                     WYTDAEERGRQLFFFEEAQSLNHPGCSHCHFAPTFGNNAFTTYANNGLDNVSNLDAFA
                     DKGRGEVTGNRFDNGKFRVVSLRNIELTAPYMHDGRFQTLEQVLDNYSIGGHGVENED
                     VNILPFTLSAQDKQDLIAFLKTLTDPSFINNPKFSDPFK"
     gene            2404..3471
                     /locus_tag="PK198_12450"
     CDS             2404..3471
                     /locus_tag="PK198_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRF39590.1"
                     /translation="MNPNKVCAWLAVSLAAVFLFACKTEPKQQPDNYIVVEGNTMGTY
                     YRVTYADSAGRDFSADIDALLKQINLEISTYIDSSTISRFNKAEYMIDLGIDLEAGST
                     DNRHFWTCWWKGLEMYQKSGGAFDPTIMPLVNYWGFGYTEKRAVTKADTAAIDSLLQF
                     VGLNKVVLEKGPPAILRKSLPGVQLDFSALGQGYGVDAIAEFLEGREVKHYLVDIGGE
                     QRAKGNSSRGDAWRIGISVPQEGADKQDIITSFPLENLSVNTSGNYRNYYDVEGVKYG
                     HTISPFTGFPQKSNLLSATIFAKDCLTADVWATSCMVLGPEKAMQLVRQQPEMEALFV
                     LGTEDGGMKLEYTPGLKKFFK"
     gene            3490..4323
                     /locus_tag="PK198_12455"
     CDS             3490..4323
                     /locus_tag="PK198_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF39591.1"
                     /translation="MEYNLDIPLERDICFFDIEATGLNVIRDRILQIGIVKFFADSRP
                     PAEMYMLINPGVPISEEALSVHGISNKDVANKPTFQQVAEKIYDFIGDADLAGYNSNR
                     FDVPMLMEEFDRVGMHFDMEKRRSLDVQRIFYKMEPRTLKAALKYYCDQEMPDAHDAL
                     ADVRATIEVFKGQLNRYKGVDYIDEDGNVEKTPIKKSIPALHDFTNDLRFVDATQKMK
                     LDQNGVVVFTFGKYVGRPVGEMLSKDKQYYHWMLSKEFSSQVKQIIQKLVKEYEEGRK
                     F"
     gene            4423..5277
                     /locus_tag="PK198_12460"
     CDS             4423..5277
                     /locus_tag="PK198_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF39592.1"
                     /translation="MTTHHAILNSPSGRPFALDISFTADGQAKPVVIFLHGFKGFKDW
                     GCWNLMAGAFAEAGFVFVKFNASHNGVTIENPTEFADLEAFGHNTYSLELADLDAVLS
                     WLTEGRSPVPADEMDVSRLALLGHSRGGGVVLVQSAMDERVKAVITLAAVSNLAFMWE
                     GNPALELWKETGVTHILNARTGQQMPVYLDVYEDYQARQPQLNIADAMQRLTQPCLLI
                     HGDADASIPVQAAHQLQAWNPSAEVLVLPGADHVFGAQNPWTEAELPPHTQQATEFAI
                     AFLMDVLS"
     gene            5375..7033
                     /locus_tag="PK198_12465"
     CDS             5375..7033
                     /locus_tag="PK198_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF39593.1"
                     /translation="MELRSITIGAQLRQNAVAFPDRDAAIFNEPVARFTWKSLDAETD
                     RLAKGLIALGVQRGDHVAVWGTNVPVWLLTQLAVARIGGTLVTINPEWKEQELKYALS
                     QSDTRVLVMIPGWEKTIKDKTIRYDYLRMIQSVCPELPCPDGFPELRHIIVAGETPQA
                     AGFMSWEDCLQKGQPVSDATLAAMAEVVNPDDVVLLQYTSGTTGFPKGAMLTHYNVIN
                     NALAAADHMGVHEQDRICGPVPFYHCFGSILFVLGAVVSGAAMVVPAPMFHAHKTLET
                     VEREQCTALYGVPTMFIAEMEEIDFDAFNLRSLRTGIMAGAPVDKELFEAVTQRMGAA
                     EMTIAYGLTEASPVTHQTWIVDPVEKRITTVGRPVDHTEARIVDPGTFRTLGAGEVGE
                     IWVKGWHVMKGYYKKPEETAKAIVEYGWLRSGDLGVMDEKGYYRIVGRLKEMFIVGGH
                     NVYPAEVEQSLHTILDDKVEMLQVVGVPHPKLQEVAALVVKLLPGKNLTLEEIQQRCD
                     GKLEWPKIPRYLKIMDDFSVAMTVTGKIQKFKLTELLRQEWGLN"
     gene            7099..7443
                     /locus_tag="PK198_12470"
     CDS             7099..7443
                     /locus_tag="PK198_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39594.1"
                     /translation="MQQSPFSFDQFKRTAFQQKAPKAYSEVFFQIHFDEFGASLSVTD
                     GKGKPVSASYLNYGGAVRNVLRVLEQIEDKNSFVINWDRPSDKVYLADHPYLMEALRH
                     CGNVTKWGGRIQ"
     gene            7922..8428
                     /locus_tag="PK198_12475"
     CDS             7922..8428
                     /locus_tag="PK198_12475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF39595.1"
                     /translation="MTIDQNTVVTLHYKLQEDDANGELIEDTHGGEPLTFLFGVGQMI
                     PEFEANLEGKNAGDHFAFGIAHTEAYGPYEDDAVAALPLDIFMHEGEVDREMLQPGRM
                     IPLRDEEGNMLYGTVTEVRENDVLMDFNHPMAGIDLYFSGQVQSVRAATPEELDHGHV
                     HGEGGHHH"
     gene            8601..9482
                     /gene="cysM"
                     /locus_tag="PK198_12480"
     CDS             8601..9482
                     /gene="cysM"
                     /locus_tag="PK198_12480"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HRF39596.1"
                     /translation="MSAILDLVGNTPMVQIRRLIHKPGVQVFAKLEGHNPGGSVKDRA
                     AYGMIKGALDRGDIRPGMKLVEATSGNTGIALAMIARLFDLDITLVMPDNSTRERVQT
                     MQAFGAEVILTPAARSIEFSREYAEELAGTGRYFMLNQFANPDNWGMHYRSTGPEIWR
                     DTQGAVTHFVSSMGTTGTIMGTSRYLKEQNPSVQIVGVQPVEGSNIPGIRRWSPEYMP
                     AIYEPARVDRIIDVTQEEATHTMRRLAQEEAIFGGMSSGGAVAAAARLAQELDSGLIV
                     CIICDRGDRYLSSGIFG"
BASE COUNT         2298 a         2842 c         2585 g         2031 t
ORIGIN      
        1 cctgaaactg ccacaggagg tagtcggcgt aggtgtagtg gccttccggg tcgaggtcgg
       61 aaaactgcgt gacgattttg ggaagcgtgg cggtactcat tttgcaatgt tgttgtgccg
      121 tgcaaatata cgcagttgtg ccgataaaat catcacccga tttgcacgga ggtcatgttc
      181 agcgagccgc cgatgacgcg gtcgttgaaa atacggacgg gttcgtcgcc ctgcttgcct
      241 gccagcacat acagcagggg caggtagtga tcgggcgtgg ggatggccag gtcgaaggct
      301 ttgccccggt tgcggaaagc cgtcaatgcc tcgtggtctc ctgtggcgat gagctttttc
      361 atgctttcgt tggcctctat ggcccaatca tagccgtatt ccggcaggtg gagcttgtcc
      421 caggctacca tgcgcaggtt gtgaaccatg ttgccgctgc ccacaatgag cacgcccttg
      481 cgccggagcg ccgccagttc acgccccagt tcgtagtggt attgtggcgt ttgaaaacgg
      541 tccaaactca gttgaaaaac aggcacatcg gcattggggt acaaatgctt gaccacgctc
      601 caacagccat gatcgaggcc ccactgctgg tccatgccca cctgcgtaga tgtgatggtt
      661 ttttgggttt ccagagccat ttccgggctg ccgggcgccg ggtattgcac atcgtacagc
      721 gcttgcggga aaccgccgaa atcgtggatg gtgcgcggct gctgcatggc cgtgacccag
      781 gtgccccgcg tttcccaatg cgccgacacg cagagaatgg ccttggggcg cggtatggac
      841 tcgccgattt tgcgccagtt ttgagaaaac tcattggttt cgatggcgtt catcgggctg
      901 ccgtgcccca aaaagagcac aggcatcaag ccggtggatg gaagttcgga cacactgtct
      961 ccaaaagttc tgagtttcat aagaatacct gtcgccggca acaaggccag ccatttgaga
     1021 aaggtttttc tttccatgat gaaggaatgt gttggtcact cgcagctttc atacaatctc
     1081 ttaccctcgt aaaataaatg tcgctacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnntttagc cgacaagctc ttgaaaaatc gctcgctgtt caatacattt
     1201 tgcatcggcg ttgttctatg aactgaaaat ctacaacatg aagcgcgttc atttattctt
     1261 tctgctattg atattttggg ctgtctggac ggcatcgggc tgcaacccgg acgaccccga
     1321 cccacccaaa gacgaactca tcagcggcga atacgccccg caatctgtga cgctgaccgt
     1381 gcccaactgg atgcctcagc cgctcattcc cgccgacaac ccgctgacgg aggagggcat
     1441 agaactgggc cgccgattgt tttacgaccc cattctctct tccgacagca cgcaatcctg
     1501 tgcctcctgt caccggcagg aaaaggcatt caccgacggc ttagcagtga gtaccggtgt
     1561 gaggggtttg cccggccggc gcagcgctat ggctttggac aacctgagtt tccatgcccg
     1621 cgagttcttc tgggacggca ggtcgccctc gttggaggcg caggcgcttg tgcctgtgga
     1681 agacccgctg gaaatgaatg aaacctggcc caacgtattg gaaaaactgc gtcgccaccc
     1741 cgattacccc cggcgattcc gggccgcatt cggcattgaa aagaaaagcg aaatcaccaa
     1801 agagttagct gccaaggcca ttgcgcagtt tgagcgcacc ctcgtgagcg ccaactcccg
     1861 ctacgaccgc gtgatctggc tcaacgacgg ctggtacacc gatgccgaag agcgcgggcg
     1921 gcaattgttt ttctttgaag aggctcaaag cctgaatcac cccggctgct cgcactgcca
     1981 ttttgcgccc acgttcggca acaacgcctt caccacctat gccaacaacg gcttggataa
     2041 cgtgtccaac ctcgacgctt ttgccgacaa aggccgcggc gaagtgaccg gcaaccgatt
     2101 cgacaacggc aagtttcggg tggtctcttt gcgaaacatc gaactgacgg cgccctacat
     2161 gcacgacggc aggtttcaaa cgctggaaca ggtattggac aactacagca tcggcgggca
     2221 tggcgtggaa aatgaagatg tcaacatttt gcccttcacg ctcagcgcgc aggacaaaca
     2281 agacctcatc gccttcctga aaacgctgac ggatccgtca tttatcaaca atcctaaatt
     2341 cagcgacccg ttcaagtgag gaaaacggtt acatttgcgg cctcaacctc aaagaggact
     2401 tttatgaatc caaataaagt atgtgcatgg ctggctgtat ctctggcagc cgtcttcctg
     2461 ttcgcctgca aaaccgaacc caaacaacaa cccgacaact acatcgtggt ggaaggcaac
     2521 acgatgggta cttattaccg cgtcacttat gccgattctg ccggacgtga tttcagcgca
     2581 gacatagatg ccctgctcaa acagatcaac ctcgaaattt ccacttacat tgacagttcc
     2641 accatttccc ggttcaataa ggccgagtac atgattgatc tgggcattga tctggaagca
     2701 ggcagcaccg acaaccggca tttttggacc tgctggtgga agggcttgga aatgtatcaa
     2761 aaatcgggcg gcgctttcga tcctaccatc atgcctttgg tcaattattg gggctttggt
     2821 tatacagaaa agcgcgctgt tacaaaggcc gacaccgctg ctattgattc tttgctccaa
     2881 ttcgtgggcc tgaacaaagt ggtattggaa aaaggcccgc cggccatcct ccgaaaatca
     2941 ttgccgggcg ttcaactcga cttcagtgcg ctgggccagg gctacggcgt ggatgctatt
     3001 gccgagtttc tggaggggcg cgaggtgaag cattatctgg tggacatcgg cggcgaacaa
     3061 cgagccaaag gcaacagctc tcgcggcgat gcttggcgca taggcatcag cgtgccgcag
     3121 gagggcgccg acaaacaaga cattatcacg tcgtttccat tggaaaacct ttcggtcaat
     3181 acctccggca actaccgcaa ttactacgat gtggagggcg tgaaatatgg ccacaccatc
     3241 agcccgttta ccggttttcc gcaaaaatcc aacctgctga gtgccaccat ttttgccaaa
     3301 gactgcctca ccgccgacgt gtgggccact tcctgcatgg tgctcggccc cgaaaaagcg
     3361 atgcaactcg ttcggcagca gcccgaaatg gaagccctgt ttgtcctcgg tacggaagac
     3421 ggtggcatga aattggaata tacgcccgga ctgaaaaagt tttttaaata atcaactcac
     3481 acccaactga tggagtacaa cttagacatc cccttagagc gggatatttg ttttttcgat
     3541 attgaggcca cggggctcaa cgtgatccgc gaccgcattt tgcaaatcgg catcgtcaag
     3601 ttcttcgctg acagccgccc gcctgccgaa atgtacatgc tcatcaatcc gggcgtcccc
     3661 atttccgaag aggcgctctc ggtacacggc atttccaaca aagacgtggc caacaaacct
     3721 actttccagc aagtggctga aaaaatctat gatttcattg gcgatgccga tctggccggg
     3781 tacaattcca accgtttcga cgtgcccatg ctcatggagg aatttgaccg cgtgggcatg
     3841 cactttgaca tggaaaagcg ccgcagcttg gatgtacagc gcatttttta caaaatggaa
     3901 ccgcgcaccc tcaaggctgc tctgaaatac tattgcgatc aggaaatgcc cgacgcgcac
     3961 gatgcgttgg ccgatgtacg cgccaccatc gaggtgttca aaggccaact caaccgctac
     4021 aaaggcgtgg actacataga cgaagacggc aatgtggaaa aaacgcccat caaaaaaagc
     4081 ataccggcac tgcacgactt caccaacgac ctccgctttg tggatgccac tcaaaaaatg
     4141 aaactcgacc aaaacggcgt cgttgttttt actttcggga aatatgtagg ccgcccggta
     4201 ggcgaaatgc tctcgaaaga caagcagtac taccactgga tgttgagcaa ggagttctcc
     4261 tcgcaggtca aacagatcat ccagaaattg gtgaaagaat acgaagaagg ccgtaaattc
     4321 tgacgaaaaa agtttctatg cgtagtgaag ggctgactac ggtgccgaat caccggccaa
     4381 gtcagccctt cactgcccac tgtcaatcag cacatttatt taatgacaac gcatcacgcc
     4441 atactcaaca gcccatccgg ccggcccttc gcattggata tatcgtttac cgccgacggg
     4501 caggccaagc cggtagtcat ttttctgcat ggtttcaaag gcttcaaaga ctggggctgc
     4561 tggaatctga tggccggggc ttttgcggaa gcgggttttg tttttgtcaa attcaacgcc
     4621 tcgcacaacg gagtcaccat tgaaaacccc acggaatttg ccgatctgga agccttcgga
     4681 cacaacacct actcgctgga actggccgat ttagatgccg tcctttcctg gctgaccgag
     4741 gggcgctcgc ccgtgccggc tgatgaaatg gacgtttccc gattggcctt gctgggccac
     4801 agccggggtg ggggagtggt gctggtgcaa agcgcaatgg acgagcgcgt gaaagccgtg
     4861 ataacgctgg ccgccgtgtc caacctggcg tttatgtggg agggcaatcc cgcgctggag
     4921 ctttggaaag aaaccggcgt cacgcacatc ctcaatgccc gcaccggaca gcaaatgccg
     4981 gtgtatcttg atgtgtacga agattatcag gcccggcagc cacaactcaa catcgctgac
     5041 gccatgcaac ggctcaccca accctgcctc ctcatacacg gcgatgcaga tgcctccatt
     5101 ccggtgcagg ccgctcatca attacaagcc tggaatccct ccgccgaagt cttggtgctg
     5161 cccggcgcag accatgtttt cggcgcacaa aacccctgga cggaagccga gttgcctcct
     5221 cacacacagc aggccactga atttgccatt gcatttttaa tggacgtttt atcttgaccg
     5281 aaaaatgaca cagaacatta ttctttgtca ttgtacaata cctctgcaat tccgtatttt
     5341 tggcaagtaa tattagccaa ctaaagccaa aactatggaa ttgcgttcaa tcaccatcgg
     5401 cgcacaactg cgacaaaatg cggttgcctt ccctgaccgg gatgccgcca ttttcaacga
     5461 acctgttgcc cgtttcacct ggaaatcttt ggatgccgaa accgaccgcc tcgccaaagg
     5521 gctgattgcg ctcggcgttc agcgcggcga tcatgtggcc gtctggggta ccaacgtgcc
     5581 tgtgtggctg ctcacccaat tggccgtagc ccgcatcggc ggcactttgg tcaccatcaa
     5641 cccggaatgg aaagagcagg agttgaaata tgcgctctcg cagtccgaca cccgtgtgct
     5701 cgtcatgatc ccaggttggg aaaaaaccat caaagacaag acgattcgct acgattacct
     5761 gcgcatgatt cagtcagttt gccccgaatt accctgccct gacggcttcc ccgaactccg
     5821 gcacatcatc gtagccggtg aaaccccgca ggccgcaggt tttatgagtt gggaagactg
     5881 tctgcaaaag gggcagcccg tttccgatgc aaccctggcg gctatggcag aggtcgtaaa
     5941 ccccgacgac gtggtgttgc tgcaatacac atccggcact acgggtttcc ccaaaggcgc
     6001 tatgctgacg cactacaacg tcatcaacaa tgcgctggcc gccgccgatc acatgggcgt
     6061 tcacgagcag gatcgcatct gcgggccggt gccgttttat cattgtttcg gctccatttt
     6121 gttcgtgttg ggcgcagtgg tcagcggtgc ggccatggtt gttcccgccc ccatgtttca
     6181 tgcgcacaaa acattggaaa ccgttgaacg cgagcaatgt acggccttgt acggcgtgcc
     6241 caccatgttc atagccgaga tggaagagat agatttcgac gccttcaacc tgcgctcgct
     6301 gcgcaccggc atcatggccg gcgctccggt ggacaaagag ctgtttgagg cggtcaccca
     6361 gcgcatgggc gccgctgaaa tgaccattgc ttatggcctc accgaagcct cgccggtaac
     6421 gcaccaaacc tggatcgttg accccgtgga aaaacgcatc accaccgtgg ggcggcccgt
     6481 agatcacacc gaggcccgca tagtggaccc cggcactttc cgcacgctgg gcgccggcga
     6541 agtaggcgaa atatgggtga agggctggca tgtgatgaaa ggctactaca aaaaaccgga
     6601 agaaaccgcc aaggccattg tggagtatgg ctggctgcgc agcggcgacc tcggcgtgat
     6661 ggacgaaaaa ggctactacc gcatcgtggg ccgcctcaaa gaaatgttca tcgtgggcgg
     6721 gcacaatgtt tacccggccg aggtggagca atcgctgcac accatcttgg acgacaaggt
     6781 ggagatgctg caagtggtgg gcgtgccgca tccgaaattg caggaagtgg cggcactggt
     6841 ggtcaagctc ttgcccggca agaacctcac gctggaggaa atacagcagc gctgcgacgg
     6901 caagctggaa tggcccaaaa taccgcgtta cctcaaaatt atggacgact tttcggtagc
     6961 aatgaccgtg acgggcaaaa tccagaagtt caaactgacg gagctgctgc ggcaggaatg
     7021 gggtttgaat tgataactct tcacaaaatc aaatccaacc cgtaacttcg ccgcctcaac
     7081 ccacaacgag ccggcaccat gcagcaatcg ccattcagtt tcgatcagtt caaacgcact
     7141 gcttttcagc aaaaagcgcc caaagcctac tcggaggtct tttttcagat acattttgac
     7201 gagttcggcg catcgctttc cgtcaccgac ggcaagggga aacccgtttc tgccagctac
     7261 ctcaactatg gcggcgcggt gcgcaacgtg ctccgcgtgc tggagcagat agaagacaaa
     7321 aacagcttcg tcatcaactg ggaccgccct tccgacaagg tctatctggc agatcatccc
     7381 tatctgatgg aagcgctgcg gcactgcggc aatgtgacca aatggggggg gcgcatccag
     7441 tgaaggtagg attgcgacaa accgaaatac ccagcttcaa atgcttctga taaaatgggg
     7501 gggcgcatcc agtgaaggta ggattgcgac ccatactttt ttactcctaa gtcggcggcg
     7561 acgtatgggg gggcgcatcc agtgaaggta ggattgcgtc ccgcctgccg ccgaaagaag
     7621 gttctttgag gatgaaatgg ttttccatcg gcggcgcggg attcagcagc ggcgggcatc
     7681 gggttgagga cacaggcccg ccactgcttc aacacttcaa atgacagtga ccgggaatta
     7741 cctgtcttga gaaaacagtc atacttatag ctcgcagctt gagccgcgcc gtggcgcggt
     7801 tgaggctcgc agcccacagc ccccaacttg cagtttttcc tccccactcc ccactttttc
     7861 ctacctttgc gccgcccttc taccaaacgg gcattttctt catcaatctt acaaccacaa
     7921 catgactatt gatcaaaaca cggtggtcac gctccactac aaacttcagg aagacgacgc
     7981 caacggcgaa ctcatcgaag atacgcacgg cggcgaacca ctcactttcc ttttcggcgt
     8041 aggccagatg atccccgaat ttgaagccaa tctcgaagga aaaaatgccg gcgatcactt
     8101 cgctttcggc atcgcgcata ccgaagccta cggtccttat gaagacgacg ccgtagccgc
     8161 gctccccctc gacatcttca tgcacgaagg cgaggtggac cgcgaaatgc tccagcccgg
     8221 ccgcatgatc cccctccgcg acgaagaagg caacatgctc tacggcaccg ttaccgaagt
     8281 gcgcgaaaac gacgtactca tggacttcaa ccaccccatg gccggcattg acctctactt
     8341 cagcggtcag gtccaatccg tacgcgccgc tacgcccgaa gaactcgatc acggccatgt
     8401 gcatggcgaa ggcggccatc atcactgatc accgggtatc gtgaaggggc gactatttta
     8461 gtatttacaa gaaaagtcac cccttcacca cataggaaaa tcgcgattgg caaccttccc
     8521 aactggacaa tctaatttca caacgagtaa ccaagattat ggcttgcagc ttacggcttg
     8581 cagccgataa caaccagacc atgtccgcca tcctcgatct cgtaggcaac acacccatgg
     8641 tacaaatccg gcgcctcatc cacaagcccg gcgtgcaggt ctttgccaaa ctcgaagggc
     8701 acaaccccgg cggcagcgtc aaagaccgcg cagcttatgg catgatcaaa ggcgcgctcg
     8761 accgcggcga catccgcccc ggcatgaaac tcgtggaagc caccagcggc aacaccggca
     8821 tcgcactcgc catgatcgcc cgcctgttcg acctcgacat caccctcgtc atgcccgaca
     8881 actccacccg ggaacgcgtg caaaccatgc aggctttcgg agccgaagtc atcctcacgc
     8941 ccgccgcccg ctccatcgag ttctcccgcg agtacgccga agaattggcc ggcacaggcc
     9001 gctacttcat gctcaaccag tttgccaatc ccgacaactg gggcatgcac taccgcagca
     9061 ccggccccga aatctggcgc gacacacagg gcgccgtcac gcatttcgtc agctctatgg
     9121 gcaccaccgg caccatcatg ggcacctcgc gctacctcaa agagcaaaat ccgagcgttc
     9181 aaatcgttgg cgttcaacct gttgaaggct ctaatatccc cggcatccgc cgctggtcgc
     9241 ccgaatacat gcccgccatt tacgagccgg cgcgcgtaga tcgcatcata gacgtcacac
     9301 aggaagaagc cacccacacc atgcgccgct tagctcagga agaagccatt ttcggcggca
     9361 tgagcagcgg cggagcagta gccgctgcag cccggctggc ccaggaattg gacagcggcc
     9421 tcatcgtctg catcatctgc gaccgcggcg acaggtacct gtcttccggc attttcggct
     9481 gatgtttttt tggggcgtgc cggcgctccg gtagaaacca atgcgccgtc gggcgcctca
     9541 gtcgctccta aggtcactac cttcggcccc ctcacgcaac gggcgagtac tggtcttcgg
     9601 taggcggtag gcagtgggcg gtagccgcgc tgtggcgcgg ggtaggcggt actcagcgcg
     9661 gcttgcagct tgtagccggc agccgtcccc accccgcctt cccaccccac aggcgtggtt
     9721 cggcggatga aaccgaaatc ggaaatccga aatccgaaat caggaatccg aaatcaggaa
     9781 tcggaaatcc gaaatcagga atc
//
LOCUS       DAORLF010000143         9765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3731_length_9765_cov_1.960247, whole genome shotgun sequence.
ACCESSION   DAORLF010000143 DAORLF010000000
VERSION     DAORLF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_9765_cov_1.960247"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK198_12485"
     CDS             <1..287
                     /locus_tag="PK198_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF39597.1"
                     /translation="LPESKAELDRLQNLLKAEPALRLQINGHTDNVGSDAANQRLSEL
                     RAKAVCDYLILGGIAPERLRYKGFGETMPVAPNDTEEGRRRNRRTEFVAF"
     gene            506..>978
                     /locus_tag="PK198_12490"
     CDS             506..>978
                     /locus_tag="PK198_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39598.1"
                     /translation="MRHFIHIYLWALLMPAIAAAQNGYTHYGSIDPHAATSGLPFFDR
                     GMIILSDEDHIFLTSGYVKLEGGRSCHLIKVDKSDLDHVVNIVPLEGPQGDIAYVEGC
                     VSPDGGLVFTGEWRDYGIGRMRPFLAKYSTDLELQWVNYFYELDTTGIGYYPKDV"
     assembly_gap    979..988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <989..>1279
                     /locus_tag="PK198_12495"
     CDS             <989..>1279
                     /locus_tag="PK198_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39599.1"
                     /translation="HDGGFFIYYTETRVPGTPKTETAWVLKADEYGERLFHKMIPDTF
                     SSSRVWGNITPTEDGHYFVSGYGRGNWYHSQAYKIDEDAEIIWYKAGEQRQMF"
     assembly_gap    1280..1364
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1365..>1633
                     /locus_tag="PK198_12500"
     CDS             <1365..>1633
                     /locus_tag="PK198_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39600.1"
                     /translation="VYPQLDRQFNMLFAYDDQGNLAWRHGWYDRNIVTSAVYDLSPAA
                     NGDLLGVGYITLRRYPSPYFGPWVFRMDPQGHQRWERFYNDTLVRS"
     assembly_gap    1634..1738
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1739..>1981
                     /locus_tag="PK198_12505"
     CDS             <1739..>1981
                     /locus_tag="PK198_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39601.1"
                     /translation="DLPLGYQANVLLLVLDSMGCLVPGCEEGEQMITAVREPLVVAQY
                     PVSRLTVSPNPASDRVEVGLAQGFVPPSQDMVLECHD"
     assembly_gap    1982..2101
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2136..>2349
                     /locus_tag="PK198_12510"
     CDS             2136..>2349
                     /locus_tag="PK198_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39602.1"
                     /translation="MKKTILIALLSGALVSLLSAQGADFAPVGARWYVNQIVLEPIPA
                     DSFVIVEVTGEEMMAGQLCRVISNLSG"
     assembly_gap    2350..2359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2360..3041
                     /locus_tag="PK198_12515"
     CDS             <2360..3041
                     /locus_tag="PK198_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39603.1"
                     /translation="PNPAHVFTRNDSVFFYSAVTQQFELLYDFTAEVGSQWTVRGLSH
                     LSFDVQVDVLEVTYRHFGADSLKRWRVNTTGYFWGDMILEKVGSQWYPGPTSIDGCSA
                     GLEERLPYSVRCYEDDSGLYNLSVNNMPCDFFDDISSTHQDETPSGIFGVYPNPAQGR
                     VYFEMSPRLHHATLMVYDLTGRQAGTYELRGQQGSIDISAIPGHLCFWQLVHEGETIQ
                     TGRLAIQR"
     gene            3109..3519
                     /locus_tag="PK198_12520"
     CDS             3109..3519
                     /locus_tag="PK198_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRF39604.1"
                     /translation="MKDLRDKLWQLLRMKMRFATTGAIATGVDYVIYIALVDRVFSPV
                     VSNLISYPCGVIVNFTLHRKFVFQVRGPAWRAFLLSVLVSVGGLGLSTLLIHTLSQME
                     FFAAHQPITKLASASVVFFYNFYLKRFVFEGKFV"
     gene            complement(3799..4953)
                     /locus_tag="PK198_12525"
     CDS             complement(3799..4953)
                     /locus_tag="PK198_12525"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HRF39605.1"
                     /translation="MKRSQVISAIKKQISDLLSTRLDERYVYHNLQHTLAVRDACLLL
                     ADKTGLPDEDREVLELAALLHDTGFVEKYDGHEDVSCRIAEELLSQHNYPADKLSKVL
                     ACIATTQLSSQPETLLEMLMRDADLAHLASDDYFMWVAALREEWRIFQQQTYPDHEWN
                     ELNHKFLKGHSYYTNAARELWDEKKNANQKALKKLAKPADAGSDSGRITASRSAQMMF
                     KTSLRNHIDLSNLADNKANIMLSINAIIITIAMPLAATYVKANPYLLAPMAALLSTCL
                     ISMFFATMATRPIKMTGLTSDEAIQQGQANLFFFGNFFKMSFDEYRQGMQFIAADENR
                     LDSAILRDLFFLGKSLGRKYTQLRTCYTVFLSGIGITVLIFAVSYLIAGK"
     gene            complement(5089..5667)
                     /gene="nadD"
                     /locus_tag="PK198_12530"
     CDS             complement(5089..5667)
                     /gene="nadD"
                     /locus_tag="PK198_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRF39606.1"
                     /translation="MPEKTGLFFGSFNPVHIGHLIIANYMATQTELRQVWFVVSPHNP
                     LKAKQSLARDHDRLHLVRLAIGDNPRLRASDIEFKLPQPSYTIDTLAHLKEKYPDRNF
                     ALIMGSDNLATLHKWKNYELILRDYELYVYRRPDTPPTELEQHPNVHCFDAPMMHLSA
                     TYIRDCIRAGKSVQYLVPEPVFEYLESSGLYR"
     gene            complement(5670..>5965)
                     /locus_tag="PK198_12535"
     CDS             complement(5670..>5965)
                     /locus_tag="PK198_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39607.1"
                     /translation="PQEQLRLEADAELMAMLPADSTLTRTIFLKMDCKEAARDVAHCN
                     CLLEDQEGEYEAVFKLVKIEGIWRMDLPLGEEEEGVNSPALPGAKPMSLPQKL"
     assembly_gap    5966..6022
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6144..7259)
                     /gene="dnaN"
                     /locus_tag="PK198_12540"
     CDS             complement(6144..7259)
                     /gene="dnaN"
                     /locus_tag="PK198_12540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRF39608.1"
                     /translation="MRFSVSSSELLKQLQIAGGAIGSNPVLPILEDFLFSISNNKLTI
                     TASDLETSILTEMDVASDTDGKVAIPGKILMETLKALPHQPVTFSVNTENHGIEITSA
                     YGKYRLAGENGEDFPQMPEEDGVDNVSLPAAGLLDGISKTIFATSNDELRPAMTGVFF
                     QVDFNKLTMVATDAHKLVKFTFTEVASEVSTTFIVPKKALNLLKTAFSGQDQVTLSFN
                     KSNAFFTVGSTRLICRLIDARYPDYNAVIPVGNPNLLTVSRTDFQNSLKRIAIYANKT
                     TNQVILNINEGSLTVSAQDLDFSNEATEQMTCSYDGDSLTIGFNAKFLVEMLGVLESE
                     EVKMELAGPSRAGILLPTEEKPGEQILMLVMPVMLSN"
     gene            complement(7374..>7629)
                     /locus_tag="PK198_12545"
     CDS             complement(7374..>7629)
                     /locus_tag="PK198_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39609.1"
                     /translation="YWGPTWLLGKYDFTRLEDWAERILHWQSMGLKEIWFFTHEPDNL
                     RAPEAAAFLVSLLQDKKGIRIRGPKLNGLPEEQEGQLSLF"
     assembly_gap    7630..7639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7640..>7983)
                     /locus_tag="PK198_12550"
     CDS             complement(<7640..>7983)
                     /locus_tag="PK198_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRF39610.1"
                     /translation="SGTQIAQFCDAIELLQEKLGACFLQLPPYFGPDRLGQLDAFLQQ
                     FPRSIPLAVELRHEDWFNQEDNRQRLFDLLERRHTGAVITDVAGRRDVLHGRVTTYFA
                     MVRFVGNDLHPT"
     assembly_gap    7984..8021
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8022..8368)
                     /locus_tag="PK198_12555"
     CDS             complement(<8022..8368)
                     /locus_tag="PK198_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRF39611.1"
                     /translation="MDFGKLPSIAHVHFALPADPPDNARVLSAIPTQGGDALNVYVGC
                     TGWGMKEWVGSIYPPKTQAKDYLKHYARQFNTIEHNTTHYRIPDAATVQKWYADAAPD
                     FRFCPKIPQTISH"
     gene            8459..9055
                     /locus_tag="PK198_12560"
     CDS             8459..9055
                     /locus_tag="PK198_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRF39612.1"
                     /translation="MSENNKKSGASDAAHTKRQSQAKVLRVFRKIHRWTGAALFVFFF
                     VVALTGLLLGWKKHSGGLLLAPTYKGTTTEMTRWLPVDSLHGIACRALHREVDASLSL
                     KMDRIDIRPDKGMAKFVFADHFNGIQLDMATGEVLHIEKRRADFIEKIHDGSILDHYL
                     GTGGGQIKLFYTTVMGLALTVFTVTGFWLWYGPKRMRK"
     gene            9108..9578
                     /gene="bcp"
                     /locus_tag="PK198_12565"
     CDS             9108..9578
                     /gene="bcp"
                     /locus_tag="PK198_12565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRF39613.1"
                     /translation="MPKITLQAGDPAPDFKGIDENGQPVALSDFRGKKLILFFYPKDN
                     TPSCTTEACNLRDNYELLSRQGFEMLGVSPDSPASHQRFKTKHRLPFPLLADTDKAVL
                     KAYGAWGPKALYGRLFDGVLRTTVVIDEEGRIAKVFDKVKTGDHTRQILEGLQG"
BASE COUNT         2016 a         2303 c         2731 g         2280 t
ORIGIN      
        1 ttttgcccga atctaaagcc gaattggacc gcctgcaaaa cctgctcaaa gccgaacctg
       61 ctttgcggtt acaaatcaac ggtcacaccg acaatgtagg ctccgacgcg gccaatcagc
      121 gcctgtcgga actgcgcgcc aaggcggtgt gcgattatct catcctcggc ggcattgcgc
      181 ccgaacgcct gcgttacaaa ggctttggcg aaaccatgcc ggtagccccc aacgatacag
      241 aggagggcag aaggaggaat cgccgaacgg aatttgtggc tttttgagga gatactccta
      301 tcattgtatt ttttcttttg ttgttaaaat ttataaccga ccctgcattg ttgggtgaaa
      361 cagtttatta ttgcattcat aaagcagcct tgtcgtagtg aacccgcaaa cttagtaatg
      421 actgctccct gtattgcctg acattgattt gggcagatgt tttatgcaaa tttgatttgt
      481 atcagactct tcaaaatctt tgatcatgag acattttatt catatctact tgtgggcgct
      541 actgatgcct gccatcgccg ctgcacaaaa cggttacacc cactacggct ccattgatcc
      601 acatgcggcg accagcggat tgcctttttt tgacagggga atgattatac tttctgatga
      661 agatcacata ttcctgacaa gtgggtatgt aaagctggaa ggaggccggt cctgccactt
      721 gataaaagtg gacaaatctg atctggacca tgtagtaaac atagttccat tggaaggccc
      781 gcagggcgac attgcttatg tagagggctg cgtatctccc gacggcggct tggtttttac
      841 aggcgaatgg cgagattacg ggattggcag gatgcggccg tttttggcca aatacagcac
      901 cgatctggaa ctgcaatggg tcaattattt ttatgaatta gatacgacgg gtatcgggta
      961 ctatcccaaa gatgtgtgnn nnnnnnnnca tgacggcggc ttttttatct attatacaga
     1021 aaccagagta ccgggtacgc ccaagacgga aaccgcctgg gtgctgaaag ccgatgaata
     1081 cggcgaacgg ctgtttcata aaatgatacc ggacactttt tcctccagcc gagtgtgggg
     1141 caacattact cctacggaag acgggcacta ttttgtgagc ggatacggca ggggcaactg
     1201 gtatcattct caggcatata aaatagatga agacgctgag atcatctggt acaaagcggg
     1261 ggaacagagg caaatgttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttat ccgcaattgg
     1381 accgacagtt caatatgctc ttcgcctacg acgatcaggg caatctggca tggcgccatg
     1441 gctggtacga ccgcaacatc gttacctccg cagtgtatga tctttcgccc gcggccaacg
     1501 gcgacctttt aggggtaggg tatattacac ttcggcgtta tccgtctcct tactttggcc
     1561 cctgggtgtt tcgcatggac ccgcaggggc atcaacgatg ggagcgattt tacaacgaca
     1621 cgctggtgcg ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     1741 cctgcccctc ggctatcagg ccaatgtatt gctgttggtg ctggacagta tggggtgttt
     1801 ggtgccgggc tgcgaggaag gcgagcagat gataacggcc gtgcgggagc cattggtggt
     1861 agcccaatac cctgtgtctc gcctgacggt atcgcccaac cctgcatcgg acagggtgga
     1921 agtcggctta gcgcagggct ttgttccccc ttctcaggat atggtactgg aatgccacga
     1981 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 ncagggtgct tattgttcgt taaaaccatt gtcttatgaa aaaaacaatc cttattgcat
     2161 tgttgtccgg ggcgcttgta tcgctgctgt cggcacaagg cgccgatttt gcgcccgtag
     2221 gcgcccgctg gtacgtcaac cagatcgttt tggaacccat acctgccgat tcgtttgtca
     2281 ttgtagaggt gacgggcgag gaaatgatgg cggggcagtt gtgccgagtg atctccaacc
     2341 tgagcggctn nnnnnnnnng cccaatccgg cgcatgtttt cacccgaaat gattctgtgt
     2401 ttttctacag cgcggtaact caacagtttg agttattgta cgacttcaca gcagaggtcg
     2461 gcagtcagtg gacggtgagg gggctttcgc atctgagctt cgacgtgcag gtggacgtgc
     2521 tggaggtgac ttaccggcac tttggcgccg actcgctcaa acgctggcga gtaaatacca
     2581 ccggttattt ttggggagac atgattctgg aaaaagtcgg cagccagtgg taccccggcc
     2641 ctaccagcat tgatggttgt tcggcagggc tggaagaacg cctgccttat tccgtcagat
     2701 gttacgaaga cgattccggc ttgtacaatc tgagtgtcaa caatatgccc tgcgattttt
     2761 tcgacgatat atcttccact catcaggatg agactccttc aggtatcttc ggggtgtacc
     2821 ccaaccctgc acaagggcga gtgtattttg aaatgtcgcc ccgactgcac catgccactc
     2881 tgatggttta tgatttgaca ggacggcagg ccggcactta tgagttgcgc gggcagcagg
     2941 gcagtataga catttcggca ataccgggcc atctttgctt ctggcaattg gtgcatgaag
     3001 gcgagaccat ccaaacgggg cgattggcga tacagcgttg aagggggggg gtattgattg
     3061 aatttctttc catgcaggag cttcccgaat cattaccttt gcgctcaaat gaaagacctg
     3121 cgcgataaac tttggcagct cctgcgcatg aaaatgcgtt ttgccacgac gggggccatt
     3181 gctaccgggg tggactatgt catctacata gcgctggtgg accgggtatt ctcgccggta
     3241 gtcagcaatc tgatttcgta tccctgtggc gtcatcgtca attttaccct gcacaggaag
     3301 tttgtgtttc aggtgcgcgg cccggcctgg agggcgtttt tgctgtccgt tttggtgtcg
     3361 gtgggcgggc tgggattgag tactttgctc attcacacgc tttcgcaaat ggagtttttt
     3421 gcggcgcatc agcccattac gaaattggcc tccgcctcgg tcgtgttttt ttacaatttc
     3481 tacctcaagc gttttgtgtt tgagggaaag ttcgtttaat cggttggcag tatgcggtgt
     3541 gcagcgcctc acgagaagca accggctacc gagaaccgct gactgcctac tgaagaccgc
     3601 ctaccgattc cgggttcaat gtttcgggtt ttcgacgctg aaagacgcag gtttttatga
     3661 tcgttatgtc cctctccgag cagcgaaaat cttacaaatc ataaagatca gcgtcaaaaa
     3721 aaatcgcgtt caaggtttcg ggttcaaggt ttcgggttgc tcacccctca catactcacc
     3781 cactcacccc tgaaaacttc acttacctgc aatcaaataa ctcactgcaa aaatgagcac
     3841 agtaatgccg atgcccgaca ggaacaccgt gtagcaagtg cggagttggg tgtatttgcg
     3901 ccccagcgac ttgcccagaa agaacaaatc ccggaggatg gcgctgtcca aacggttttc
     3961 atcggcggcg atgaactgca tgccctggcg atattcgtcg aaagacattt tgaaaaaatt
     4021 gccgaagaaa aacaaattgg cctggccctg ctgtatcgct tcgtcggagg tgaggcccgt
     4081 cattttgata gggcgggtgg ccatcgtggc aaaaaacata gagatgaggc aggtgctgag
     4141 cagggctgcc atgggcgcca gcaggtaggg attggccttg acgtaagtgg cggccagcgg
     4201 catggcaatg gtgatgatga tggcattgat actgagcatg atgttggctt tgttgtcggc
     4261 cagattgctc aggtcaatat ggttgcgcag cgaggttttg aacatcatct gtgcgcttcg
     4321 ggaagcggtg atgcggccgc tgtcggagcc ggcgtcggcc ggttttgcca attttttcaa
     4381 tgccttttgg ttggcatttt ttttctcgtc ccacagttca cgggccgcat tggtgtagta
     4441 gctgtggcct ttgaggaact tgtggttcag ttcgttccac tcatggtcgg ggtaagtttg
     4501 ctgctgaaaa atgcgccact cttcgcgcag ggcggccacc cacataaagt agtcgtcgga
     4561 ggccaagtgc gccaggtcgg catcgcgcat gagcatttcc agcagggttt cgggctgcga
     4621 actgagttgg gtagtggcaa tgcaggccaa cactttgctc agcttatcgg cggggtagtt
     4681 gtgttgcgaa agcagttcct ctgcaattct gcaactgacg tcttcgtgcc cgtcgtattt
     4741 ttccacaaaa cccgtatcgt gcagcagggc ggccaattcc agcacctcgc ggtcttcatc
     4801 gggcagcccg gttttatcgg ccagcaacag gcaggcatcc cgcacggcga gggtgtgttg
     4861 gagattgtga tagacatacc gctcgtcgag ccgggtgctc aacaaatcgg atatttgttt
     4921 tttgatggcg gagatgacct gcgagcgctt cataatgtaa tcttcaagat gattccggtc
     4981 ggcaaacata cgcatccggc gcaagatttc agcctgcttt tgtcggtgcg gggtcgcctg
     5041 ctttagtttt cggtatccat tggggcaaaa atgaggccac gttcctgctc agcgatacaa
     5101 tccgctgctt tccaagtatt caaaaaccgg ctccggcacc aagtattgca ccgactttcc
     5161 ggcccggata cagtcgcgga tataggtggc cgagaggtgc atcatgggcg cgtcgaagca
     5221 atgcacattc gggtgctgct ccaattcggt gggcggcgta tcggggcggc gatagacgta
     5281 cagttcataa tcgcgcagga tgagttcgta gtttttccac ttgtgcaggg tggccagatt
     5341 gtcgctgccc atgatgaggg cgaagttgcg gtcggggtat ttttctttaa gatgggcgag
     5401 ggtgtcaatg gtgtaggacg gctgcggcag tttgaactcg atgtcggagg cgcgcaggcg
     5461 ggggttgtcg ccgatggcca agcgcaccaa atgcaggcgg tcgtgatcgc gggcgaggct
     5521 ttgcttggct ttgagcggat tgtgcggcga caccacaaac cacacctgcc gaagctcggt
     5581 ctgggtggcc atgtaattgg caatgatgag gtggccgatg tgtaccggat tgaaagagcc
     5641 gaaaaacagg ccggtttttt cgggcatgtt cagagttttt gaggcagtga catgggtttg
     5701 gcgccgggca gtgccgggct gttcacgccc tcctcctcct ctccgagggg caggtccatg
     5761 cgccagatgc cttctatttt taccaatttg aagaccgcct catactcgcc ctcctgatct
     5821 tccagcaggc agttgcaatg ggccacatcg cgggctgcct ctttgcagtc catttttagg
     5881 aaaatggtgc gggtgagggt cgaatcggcg ggcaacatgg ccatcagttc ggcatctgcc
     5941 tccaaacgga gttgctcctg tggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nngatactgc cgaatgactt cctccggcga ggcggcctga
     6061 tgatggtcgc gtttgcaaga ccaggctgca acaaccgcca tgcagccgaa caggagaaac
     6121 ggagttactt tcatatttgg gtgtcaatta ctgagcatca ccggcatgac cagcatcagt
     6181 atctgttcgc ccggtttttc ttcggtgggc aacagaatac cggcgcggga aggcccggcc
     6241 agttccattt ttacttcttc ggactccagc acgccgagca tttctaccaa aaatttggca
     6301 ttgaaaccga tggtcagcga gtcgccgtcg taagagcagg tcatttgctc ggtggcttcg
     6361 ttggagaaat cgaggtcttg cgccgaaacg gtcaggctgc cctcgttgat gttgaggatg
     6421 acctgattgg tggttttgtt ggcgtagatg gcgatgcgct tgagggagtt ttggaaatcg
     6481 gtgcgcgata cggtgagcag gttgggattg cccacaggaa tgacggcgtt gtagtcgggg
     6541 tagcgggcat ctatgaggcg gcagatgagg cgcgtgctgc ccacggtgaa aaaggcattg
     6601 gatttattga aggagagcgt cacctgatcc tggccgctga aagccgtttt gaggaggttg
     6661 agcgcctttt tgggtacgat gaaagtggta gagacttcgc tggctacttc cgtaaaggtg
     6721 aatttgacca atttgtgcgc atcggtggcc accatggtga gcttgttgaa gtccacctga
     6781 aagaacacgc cggtcatggc cgggcgaagt tcgtcgttgc tggtggcaaa aatggttttg
     6841 gagatgccgt caagcagtcc ggcagcgggc agcgacacat tgtccacgcc gtcttcttcg
     6901 ggcatttgcg gaaaatcctc gccgttttcg cccgccaagc ggtacttgcc gtaagcggaa
     6961 gtgatctcaa tgccgtgatt ttcagtgttt accgaaaagg tgacgggctg atgcggcaat
     7021 gctttgaggg tttccatgag gattttgccc ggtatggcca ctttcccgtc tgtgtcggag
     7081 gctacatcca tttcggtgag gatggaagtt tccagatcgc tggccgtgat ggtgagcttg
     7141 ttgttggaaa tgctgaagag gaagtcttcc aatataggca gcacagggtt ggagccgatg
     7201 gcgccgcctg cgatttgcaa ttgcttgagc agttctgaag aagatacgct gaatctcata
     7261 tcgggcatgt gtttttatgc tgtaaaccgc aaagatacag cgccgttcat aatatctgat
     7321 agctattttc accaacatcc tgtggaaaac gcaaaaaagt gagcggtgac ttgtcaaaac
     7381 aagctcaact gcccttcctg ctcttccggc agcccgttca actttggccc gcgaatgcgg
     7441 atgccttttt tatcctgcaa cagcgacact aaaaaggccg cagcttcggg cgcccgcagg
     7501 ttgtcgggtt cgtgggtgaa gaaccagatt tctttcaagc ccattgactg ccagtgcagt
     7561 atgcgttcgg cccagtcttc gaggcgcgtg aagtcgtact tgccgaggag ccaggtcggg
     7621 ccccaatagn nnnnnnnnnc ggtagggtgg aggtcgtttc ccacaaaccg caccatcgca
     7681 aaataggtcg tgacgcgccc atgcaacaca tcccggcggc cggccacatc ggtgatgacg
     7741 gcgccggtat gccttcgctc cagcagatcg aacaggcgtt ggcggttgtc ttcctgattg
     7801 aaccagtcct cgtgccggag ttccaccgcg agcggaatgg agcgcggaaa ttgttgcaaa
     7861 aaagcatcca attggcccag cctgtcaggc ccgaaatagg gaggcaattg taaaaaacag
     7921 gcgccgagtt tttcctgcaa caactcaatg gcgtcgcaaa actgggctat ttgggttccg
     7981 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgtggctg atggtttgcg
     8041 ggattttggg gcaaaaccga aaatcgggag cggcatcggc gtaccatttc tgcacggtag
     8101 cggcatcggg tatgcgatag tgggtggtgt tgtgttcgat ggtgttgaac tgtcgggcgt
     8161 agtgtttcag gtagtctttg gcctgtgttt tgggcgggta tatgctgccc acccactctt
     8221 tcatgcccca gccggtgcag cccacataca cgttgagcgc atcgccgccc tgcgttggta
     8281 tggccgacaa tacgcgggca ttgtcgggcg gatcggccgg caatgcgaaa tggacgtgag
     8341 cgatggaagg gagttttccg aaatccataa ggcccttaaa acacttttgc cggcaaacag
     8401 tttccccact ccaagtccgt tttttttgcg ttcttgcggg ctgatttcac tcgcaattat
     8461 gtcggaaaat aacaaaaaat cgggcgcctc cgatgccgcg cataccaaaa gacagagcca
     8521 ggctaaggtg ctgcgcgttt ttcgcaagat acaccgctgg acgggggcgg cgctgttcgt
     8581 cttctttttt gtggttgccc tcaccgggtt gttgctgggc tggaaaaagc acagcggcgg
     8641 cctcctgctg gcccccacct acaaaggtac gaccaccgaa atgacccgct ggctgccggt
     8701 ggacagcctg cacggcatcg cctgccgggc cttgcatcgg gaggtggacg cctcgctttc
     8761 gctgaagatg gaccgcattg acatccgccc cgacaaaggc atggccaagt tcgtttttgc
     8821 cgatcatttc aacggcatcc aactcgatat ggccaccggc gaggtgctgc acatcgaaaa
     8881 acgccgggcc gattttattg aaaaaataca cgacggctcc atcctcgatc actacctcgg
     8941 tacgggcggc ggccagatca aattgttcta caccaccgtc atggggctgg cgctcacggt
     9001 gttcaccgtc acggggttct ggctttggta cggccccaaa cgcatgagga aatgaaccgt
     9061 tcccatatag ccgcgttcgt tgggtattca tcaaaaacaa caatgccatg ccaaaaataa
     9121 cgcttcaagc gggcgatccg gctcccgatt tcaagggtat agacgaaaac ggccagccag
     9181 tagccctctc cgatttcagg ggtaaaaagc tcattctgtt tttttatccc aaagacaata
     9241 cgcccagttg cacaacggaa gcctgcaacc tccgcgacaa ctacgaactg ctgagtcggc
     9301 agggtttcga gatgctcggc gtgagtcccg acagcccggc ttcgcaccaa aggttcaaaa
     9361 ccaaacacag gctgcctttc ccgctgctgg ccgataccga taaagcagtg ttgaaagcct
     9421 acggcgcctg ggggcctaaa gcgctgtatg gcaggctctt cgacggcgtg ctgcgcacta
     9481 cggtggtgat agacgaggag gggcgcattg ccaaagtgtt cgacaaggtg aagacgggcg
     9541 atcatacgcg gcagattttg gaggggttgc aggggtgagt gggtgagggg tgagcaagcg
     9601 aggggtgagt gttggcgcat tggagggaat ggcgtagctt tgcacggatc gagtagcttg
     9661 atgtgatgga ctgagcgccg gcggctttga tcgggcgagg cggatttcgg ccacagattc
     9721 atggtgatac tcttgaggct gtggtgaata ttgcggggag gggga
//
LOCUS       DAORLF010000144         9757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3733_length_9757_cov_2.065141, whole genome shotgun sequence.
ACCESSION   DAORLF010000144 DAORLF010000000
VERSION     DAORLF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3733_length_9757_cov_2.065141"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2877)
                     /gene="mutS"
                     /locus_tag="PK198_12570"
     CDS             complement(211..2877)
                     /gene="mutS"
                     /locus_tag="PK198_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF39614.1"
                     /translation="MARKSASANTAAPAEGNVTPLMQQYFKMKGKYPDAIMLFRVGDF
                     YETFGEDAVKTSQTLGIVLTKRNNGGSDIELAGFPFHSLDLYLPRLVRAGHRVAICEQ
                     LEKPSPLKKIVKRGVTEVVTPGVAVDDKLLDHQTNNYLAALSFGKREDVGMAFLDIST
                     GEFLVCEGDMAYADKLLQSFQPSEVLLSKERRKDFEKKFGDKFYTYALEEWVFSPDFT
                     RQKLVDHFEVQNLKGFGIEEMELAPIAAGAILHYLETTENKNLRHINAISRILPEKYV
                     WLDRFTIRNLELLYSQHETGSPLIKVLDKTVSPMGSRLLKKWVVLPLRHKRPIEERLD
                     IVAHFLRHFDLGMELEHYIRQIGDLERLISKAPLGKINPREMVQLRRALAAIEPMKTL
                     LESAGLEPLVKIAEGLNLCKSLRDQISTQIVDEPPVNLSKGGVMADGVSPELDELRRL
                     INNSKDILLEIQQREIDRTGISSLKVGFNTVFGYYLEVTNKYKDQAPSDWIRKQTLTN
                     GERYITEELKELETKILGAEDKILELETQLFEQLVFAVADFVQPIQHNAQLIARLDCL
                     LAFARVAEKHKYCRPEINDGLVIDIKDGRHPVIEQQLPLGESYVPNDVLLDSEDQQVL
                     LITGPNMAGKSALLRQTALICLMAQMGCFVPATEARIGLLDKVFTRVGASDNISSGES
                     TFMVEMNETASIMNNISDRSLLLLDEIGRGTSTFDGVSIAWAIAEYLHNNGAARPKTL
                     FATHYHELNELAGKFPRIRNYNVSVREVGNKVIFLRKLAEGGSQHSFGIHVARMAGMP
                     RSIVERAGHILQQLELKSVDDAPPAGNGPQSKRAATQQIAAQPYQLSIFETVDPTAGK
                     IKEALLDLDLNRLTPIDCMLKLNELRKMLEGE"
     assembly_gap    2964..3065
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3290..3658)
                     /locus_tag="PK198_12575"
     CDS             complement(3290..3658)
                     /locus_tag="PK198_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39615.1"
                     /translation="MSVLVLLAMTWNLGRSANVRYFREWWYDENTKSVMLYLNDVGEA
                     RQKPVSVGVSWLFFPSGEFYRHTLALPFVEQLPNNAEIKPDGGYEYYYIEESQKPTLG
                     EAYEQVKYYQWGRVLMRKKG"
     gene            complement(3631..>3983)
                     /locus_tag="PK198_12580"
     CDS             complement(3631..>3983)
                     /locus_tag="PK198_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39616.1"
                     /translation="PLYGNAYLGEATVSVAAGLVYAAFVAVLVGSLWAWYRQPEKREY
                     SFVAALSAAFVLSIAGMIAQHYLLGSVYLVHRTAVPLMPLLNGAIAAGLLLWSGKVSR
                     QIIPAPCRCLFCWP"
     assembly_gap    3984..4124
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4125..4764)
                     /locus_tag="PK198_12585"
     CDS             complement(<4125..4764)
                     /locus_tag="PK198_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39617.1"
                     /translation="MEPFATHTNRNLRLPIALTAIAAVLWCFAVAKAHFASMTHDEAS
                     TYFHHIQSGFFKCFYSPDCWKSANNHLFNTLLWQQTIKWLGPTELGMRLPNLLGHLVF
                     LGSSAFLVLRMTTRFLPGLAAFAVLNFNPFLLDFFSLARGYGLCAGFVMAAIAFWWAW
                     QQGRTDRYLVLCCAALVLAILSNFVAFNVFLAVGGATFLLSLREGRAVWQRVL"
     gene            complement(4805..>6544)
                     /gene="paaZ"
                     /locus_tag="PK198_12590"
     CDS             complement(4805..>6544)
                     /gene="paaZ"
                     /locus_tag="PK198_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRF39618.1"
                     /translation="GIGTVFAYASLRRKLPDTPWYVDGEQAKLSREGTFIGHHLMTPK
                     PGVAIHINAFNFPIWGMLEKLSVNLLAGVPAIVKPSEITSFLAEAVVRDIIASGILPQ
                     GALQLICGTGVGILDPVGPQDVVTFTGSAETGRMLKTLPSIIEHSTPFNMEADSLNAA
                     ILGADAAPGTPEFDLFIKEVRREMTAKAGQKCTAIRRIIVPEPMVDAVQEALARELVK
                     TAIGDPRAEGVRMGALVNREQAGEVRRRVEMLSAHTPIVIGDLVEFEVIGADKNKGAF
                     FAPIVMLNEKPFHFQDSHSVEAFGPVSTIMPYRSIEEAVELANMGKGSLVSTIATFDE
                     NTAREYVFGTAPYHGRILILNRESGKESTGHGSPMPLLVHGGPGRAGGGEEMGGLRGI
                     RHYLQRTAIQGHPSMITAIGGVYQQGGRQTEAAVHPFSRHFEDLFIGETLTTARHTVT
                     EADIVNFANLSGDHFYAHTDVTALEGTPFETRVAHGYWVLSKAAGLFVQAKKGPVLLN
                     YGLEECRFTKPVYPGMTIGVRLTVKEKTAQEKRDETDVRKGIVKWLVDVYDETGETVA
                     IATILTMVKMREQ"
     assembly_gap    6545..6594
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6595..6846)
                     /locus_tag="PK198_12595"
     CDS             complement(<6595..6846)
                     /locus_tag="PK198_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535882.1"
                     /note="catalyzes the ring cleavage reaction in
                     phenylacetate degradation and the formation of
                     3-hydroxyacyl-CoA from crotonyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRF39619.1"
                     /translation="MNLHNYILGRWTPHEGAGIPQYDAVTGEVICTASSDGLDFAAIA
                     DFARQTGSPALRRMTFQERGRMLKALALHLNEIKEKYYPL"
     gene            complement(7074..7772)
                     /locus_tag="PK198_12600"
     CDS             complement(7074..7772)
                     /locus_tag="PK198_12600"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRF39620.1"
                     /translation="MCTVTYLPKGPDHEWLLSSNRDEAPHRAAHGIQRRPGLLFPQDM
                     GAGGTWIAAHEQGRAAVLLNGAFGLHQRRPPYRLSRGLMVLQYFEFADVAEFVQYFEF
                     HGLEPFTFIIREPAGLYEIRWDEQHLHHKLLPSDEPHIWASATLYGPEPQQKRRRWFA
                     EWLQKDALKDQQDVLRWHRHTGDGDAWNDLVMNRNGMVQTVSITSIAQRGNAMSMIFQ
                     DLLSGNEQQESMEM"
     gene            complement(7849..8214)
                     /locus_tag="PK198_12605"
     CDS             complement(7849..8214)
                     /locus_tag="PK198_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF39621.1"
                     /translation="MDFRDLHAYKLGFELAMEIFKVSKKFPKEETYSLIDQICRSSQS
                     VCANIAEAYRKRQYPAHYASKLSDSDAENADTLAWLEFALECEYIDKNTYEDLVANNQ
                     SVGNLIGYMMNNPQKFGVK"
     gene            complement(8299..>8562)
                     /locus_tag="PK198_12610"
     CDS             complement(8299..>8562)
                     /locus_tag="PK198_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HRF39622.1"
                     /translation="ARRKAGRIVLNGVPTGVAVAPAMTHGGPFPASTDSRFTAVGIHA
                     VRRWVRPVSFQNFSDELLPDELKNKNLRGIWRLVNNSWTRDDI"
     assembly_gap    8563..8654
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8655..>9374)
                     /locus_tag="PK198_12615"
     CDS             complement(<8655..>9374)
                     /locus_tag="PK198_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF39623.1"
                     /translation="GGDTASALAAGCPVVVKAHPAHPRTSMLVAGAIDRAADTCDVPT
                     GVFQHLEDEGFDTGKYLVMHPQTRAVGFTGSLSGGMALHGYAQQRPDPIPVFAEMGSV
                     NPMLVLTEAVSKRGEAIAQTCAGSITLGMGQFCTNPGLMMGIDCMGLRHFIEVLGQHI
                     AQAKPAPMLHEGISRNYVEKLAQTLGSKGVYLAGQSDAGAPSAAVAWVSGDDFLQNPH
                     LHEEVFGPFSLMVICKDAAQLA"
     assembly_gap    9375..9472
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9473..9739)
                     /locus_tag="PK198_12620"
     CDS             complement(<9473..9739)
                     /locus_tag="PK198_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF39624.1"
                     /translation="MTIQEKVNITMQRAGAAAEAWSQSAPELRARFLETIATEMEALD
                     SILIQTAMQETNLDEARLTGERMRTTGQLRMYAAMLREGSWVEAA"
BASE COUNT         1831 a         2663 c         2660 g         2120 t
ORIGIN      
        1 atctattttt ttctctattt tacccaacag ctttactttg cccacatcac ataaccgctc
       61 gcgccgagat tccatctcgc gtgagctatt atgcgggttc tacccgcttg taaagcacct
      121 gcaaaagcga gcatctggcg ggtatgaacc cgccgaaaag tggcacgaga tggaatctcg
      181 cgccagcatg acagcgcgct tgtttgcggt ctattctccc tccagcatct tcctcaactc
      241 attcagtttc agcatacaat caatcggcgt cagccggttc aggtcgaggt cgagcagggc
      301 ctctttgatt ttgccggcgg tggggtccac ggtttcgaag atgctgagtt ggtagggctg
      361 cgcggcgatt tgctgcgtgg cggcgcgttt gctttgcggg ccattgccgg ctgggggcgc
      421 atcgtccacc gatttgagtt ccaattgttg caaaatatgc ccggcgcgct ccacgatgct
      481 gcgcggcata cccgccatgc gcgccacatg gatgccgaag ctgtgctgac tgccgccctc
      541 ggcgagtttt ctcaaaaaaa tgaccttgtt gcccacctcg cgcaccgaga cgttgtagtt
      601 gcggatgcgg ggaaatttcc cggccaattc gttgagttcg tggtagtgag tggcgaagag
      661 cgttttgggc cgcgccgcgc cgttgttgtg caagtattcg gcgatggccc aggcaatgga
      721 caccccatcg aaagtgctgg tgccgcgccc gatctcgtcc aacaggagca ggctgcggtc
      781 ggagatattg ttcataatgc tggccgtttc gttcatttcc accatgaatg tggactcgcc
      841 ggaggagatg ttgtcggaag cgcccacgcg ggtgaatacc ttgtccaaca ggccgatgcg
      901 cgcttcggtg gcgggtacga aacagcccat ttgcgccatg aggcagatga gggcggtttg
      961 gcgcagcagc gccgatttgc cggccatgtt ggggcctgtg atgagcagta cttgctggtc
     1021 ttcgctgtcc aagagcacgt cgttgggtac atacgattcg ccgaggggca gttgctgctc
     1081 aatgaccggg tggcggccgt ctttgatgtc aatcaccaag ccgtcgttga tttccgggcg
     1141 gcagtatttg tgcttttcgg ccacgcgggc aaaggccagc agacagtcca agcgggcgat
     1201 gagctgggca ttgtgctgaa tgggctgcac gaagtcggct acggcaaaaa cgagctgttc
     1261 aaagagttgc gtttcgagtt cgaggatttt atcttcggcg cccaatatct tggtttccaa
     1321 ctctttgagt tcctcggtga tgtagcgttc tccgttggtg agggtttgtt ttctgatcca
     1381 gtcggagggc gcctggtctt tgtatttgtt ggtcacttcg aggtaataac cgaagacggt
     1441 attgaatccg accttgagac tggagatgcc ggtgcggtct atttcgcgct gctgaatttc
     1501 gagcaggatg tctttgctgt tgttgatgag gcggcgcagt tcgtccaatt cgggagacac
     1561 gccgtcggcc atgacgcccc ctttggagag gttgacgggc ggttcgtcca cgatctgggt
     1621 gctgatttgg tcgcgcagag atttgcagag attcagtcct tcggcaattt tcaccaaggg
     1681 ttccagaccg gcgctttcca gcagcgtttt catgggttcg atggcggcca gggcgcgacg
     1741 caactgcacc atttcgcggg gattgatctt gcccagaggc gctttggaga tgaggcgttc
     1801 gaggtcgccg atctgccgga tgtagtgttc cagttccatg ccgaggtcga aatggcgcag
     1861 gaaatgggcc acaatgtcca accgctcttc gatggggcgt ttgtggcgca ggggcagcac
     1921 tacccatttt ttgagcaggc gggaccccat gggcgacacg gttttgtcca acactttgat
     1981 gagcggcgag ccggtttcgt gctggctgta cagcagttcg aggttgcgga tggtgaagcg
     2041 gtcgagccag acgtattttt cgggcaggat gcggctgatg gcgttgatgt gccgcaggtt
     2101 tttgttctcc gtagtttcca agtagtgcag gatagcgccg gccgcgatgg gcgccaattc
     2161 catttcttcg atgccgaagc ctttgaggtt ttgcacctcg aagtgatcca cgagtttttg
     2221 gcgggtgaag tcggggctga acacccattc ttcgagggcg taggtgtaaa acttgtcgcc
     2281 gaattttttt tcaaaatcct tgcgccgttc tttggaaagc aacacctccg aaggctggaa
     2341 actctgcaaa agcttgtcgg cgtaagccat atcgccctcg cacaccaaga actcgccggt
     2401 ggagatgtcc aaaaaagcca tgcccacgtc ttcgcgtttg ccgaaagaga gcgcggctaa
     2461 gtaattgttg gtttggtgat cgaggagctt gtcgtccacc gccacgccgg gcgttacgac
     2521 ctcggtgacc ccgcgcttga cgattttttt gagcggactg ggtttttcca attgctcaca
     2581 gatggccacc cggtgaccgg cgcgtaccaa gcggggcagg tagagatcga gcgagtgaaa
     2641 ggggaagcca gccaattcga tgtcgctgcc gccgttgttg cgtttggtga gtacgatgcc
     2701 cagtgtttgc gaggttttta cggcgtcttc tccgaaggtt tcgtaaaaat cgcccacgcg
     2761 gaagagcatg atggcatcgg ggtacttgcc tttcattttg aagtactgct gcatcagcgg
     2821 cgtgacgttg ccctcggcgg gggcggcggt gttggcggag gcgctttttc tggccacagt
     2881 gttgaaattt tgaggggcaa atatagggaa tttcgggtta cggcgggttc aaggtttcgg
     2941 gttccgcgtt caaggtttcg agtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnttcgc taaaattcgc gttaaaaagc ttcgtgtttc gggttcaagg tttcgggttc
     3121 cgggttcaag gtttcgggtt ccgcgtttcg ggttccgccc gacgataaac tgcaaagcag
     3181 attaccaacc ctcacacact cacccctcac ctctgaaaac cgcctaccgc acactctacg
     3241 cttaccgcgc cacggcgcgg ccaccgccca ctccgcgcca tacccgcggc taccccttct
     3301 tccgcatcag caccctgccc cactgataat acttcacctg ctcataagcc tcgcccagcg
     3361 taggtttctg actttcttcg atgtagtaat attcgtatcc gccgtcgggc ttgatctctg
     3421 cattgttggg caattgctcc acaaaaggca aagccagcgt gtgccggtaa aactctcccg
     3481 aaggaaaaaa caaccaactc acacccacgc tcaccggctt ttggcgcgcc tcgcccacgt
     3541 cgttcaggta caacatcacc gacttcgtgt tttcgtcata ccaccactcg cggaaatagc
     3601 gcacattggc cgagcgcccc agattccagg tcatggccaa caaaacaagc accgacaagg
     3661 cgcaggaatt atctgccgag acactttacc gctccacagc aaaagccccg ccgcaatggc
     3721 cccgttcagc aaaggcatca ggggcactgc cgtgcgatgc accaaataca ccgaccccaa
     3781 gagataatgc tgcgctatca tgcccgctat gctgagtaca aacgccgccg acaaagctgc
     3841 cacaaagctg tattccctct tttcaggctg ccggtaccag gcccacaggc tgccaaccaa
     3901 taccgccaca aaagctgcat aaacaaggcc ggcggccacc gacaccgtcg cttcgcccaa
     3961 atacgcgttg ccgtacagcg ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccagca cgcgctgcca
     4141 cacggcccgc ccctcgcgca gcgacaacaa aaacgtggcg ccgcctacag ccagaaatac
     4201 gttgaacgct acaaaattgc tcagtatggc caacaccaat gccgcgcaac acagcaccaa
     4261 ataccggtcg gtgcgcccct gttgccacgc ccaccaaaaa gcaatggccg ccatcacgaa
     4321 accggcacac agcccgtagc cccgcgccaa cgaaaagaaa tcgagcaaaa aaggattgaa
     4381 attgagcact gcaaaggccg ccagcccagg caaaaagcgc gtcgtcatcc tcaaaacgag
     4441 aaacgcgctg ctgccgagga ataccaagtg ccccagcaaa ttgggcaggc gcattcccaa
     4501 ctccgtcggc cccagccatt ttatcgtctg ttgccacagc agggtattga acaggtggtt
     4561 gttggcgctt ttccaacaat cgggcgagta aaaacacttg aaaaatcccg actgtatatg
     4621 gtggaaataa gtggatgcct cgtcgtgggt catcgaggcg aaatgggctt tggcaaccgc
     4681 aaaacaccac aatacggcgg ctatggcagt gagcgctatg ggcaggcgaa ggtttcggtt
     4741 ggtatgggtg gcaaatggct ccatgtatga aagaatttgc ggccaaaggt ataggaaaat
     4801 gttcttactg ctcccgcatc ttcaccatcg tcaaaatcgt agcaatggcc accgtttcgc
     4861 ccgtctcgtc atacacatcc accaaccatt tcacaatgcc cttgcgcaca tccgtttcat
     4921 cgcgtttttc ctgcgccgtc ttttccttca ccgtcaggcg caccccgatg gtcatgccag
     4981 gatacaccgg tttcgtaaaa cggcattctt cgaggccgta attcaacaaa accggcccct
     5041 ttttggcttg cacaaacaag cccgccgcct tcgacaacac ccagtagcca tgcgccaccc
     5101 gcgtctcgaa aggcgtgcct tccagcgccg tcacatcggt atgcgcgtag aaatggtcac
     5161 cgctcagatt ggcaaaattc acaatatcgg cctccgtcac cgtgtggcgg gccgtggtga
     5221 gggtttcccc aatgaacaag tcctcgaaat gccggctgaa cggatgcacc gctgcttctg
     5281 tctgccgccc gccctgctga tacactccgc cgatggccgt tatcatgctg gggtggccct
     5341 gtatggccgt gcgttgcagg tagtgccgga tgccccgcag gccccccatt tcctcgccgc
     5401 cgcccgcccg gcccggccct ccatgtacca gcaaaggcat gggcgaaccg tgcccggtgc
     5461 tctccttgcc gctctctcgg ttcaagatga ggatgcggcc atgatagggc gccgtgccga
     5521 agacatactc gcgggctgtg ttttcgtcaa aagtggcaat ggtgctcacc aacgagcctt
     5581 tgcccatatt ggccaattcc accgcttcct ctatgctgcg atacggcatg atggtgctca
     5641 ccggcccgaa agcttccacc gagtggctgt cctgaaaatg aaaaggtttt tcgttgagca
     5701 tgacgatggg cgcaaaaaac gcccccttgt ttttgtcggc cccgatgacc tcaaactcca
     5761 ccaaatcgcc gatgacaatg ggcgtgtgtg cactgagcat ttctacgcgg cggcgcacct
     5821 cgccagcctg ctcgcgattc accaaggcgc ccatgcgtac cccttctgca cgcgggtcgc
     5881 cgatggcggt tttcaccaat tcgcgggcca gcgcctcctg cacggcatcc accattggct
     5941 ccggcacgat gatgcggcga atggcggtac atttctgtcc ggctttggcg gtcatttcgc
     6001 ggcgcacttc tttgatgaag aggtcaaatt cgggcgtacc gggcgcagcg tccgcaccga
     6061 ggatggcagc gttgaggctg tcggcctcca tgttgaacgg cgtcgaatgt tcgatgatgg
     6121 aaggcagcgt tttgagcatc cgcccggtct cggcagaacc ggtaaacgtg accacatcct
     6181 gcggccccac cggatcgagg atgcccacgc cagtaccgca aatcagttgc agcgcgccct
     6241 gcggcagtat gcccgatgcg atgatgtctc gcacgaccgc ctctgccaga aaggaagtga
     6301 tttcagaggg tttcacaatg gcaggcacgc cggctaagag gttgacagac agcttctcca
     6361 acatgcccca gatggggaag ttgaacgcat tgatgtggat ggccacgccg ggtttgggcg
     6421 tcatcaggtg gtggccgatg aaggtgccct cgcgcgagag tttggcctgc tcgccgtcca
     6481 cataccaggg cgtatcgggc agtttgcggc gcagactcgc gtacgcaaac acggtgccga
     6541 tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagcgg
     6601 gtagtatttt tctttgattt cgttgaggtg cagggccagg gccttgagca tacggccgcg
     6661 ctcctgaaag gtcatgcggc gcagggcggg gctgccggtt tggcgcgcga agtcggcaat
     6721 ggcggcaaaa tcgaggccat cgctgcttgc ggtgcagatc acctcgccgg tcacagcatc
     6781 gtattgcgga ataccggcgc cttcgtgggg cgtccatcgg ccgaggatgt aattgtgtag
     6841 attcatggtt ttgaatggtt tatggcaatt ttttttatac aatcagatgg gggcgccacc
     6901 cgcctctacc tgcctccccg accctccaca ggagggtcgg gctatccact gcatggcggc
     6961 tccccgcaga gctgttagct ttatcgggct attgccgtgc taaagctaac agctctatcc
     7021 ccgaccgccg ccatttcgtt gctatccctg gcgcgctcgc ccgccctcgt cctctacatc
     7081 tccatgcttt cctgctgttc gttaccgctc aacaaatcct gaaaaatcat gctcatggcg
     7141 ttcccccttt gggctatgct ggtgatgctc accgtttgca ccatgccgtt tcggttcatt
     7201 actaagtcgt tccaggcgtc gccgtcgccg gtgtggcggt gccagcggag aacatcctgc
     7261 tggtctttga gcgcatcttt ttggagccat tctgcgaacc accggcggcg tttctgctgc
     7321 ggttccggcc cgtacagggt ggccgatgcc cagatatgcg gttcatctga aggcaatagt
     7381 ttgtgatgca ggtgctgctc gtcccagcgg atttcgtaca gcccggctgg ctcccggatg
     7441 atgaaggtga acggctccaa tccgtggaat tcaaagtatt gcacaaactc cgccacatcg
     7501 gcaaactcaa aatattgcaa caccatgagt ccgcgactga gcctgtaagg cgggcggcgt
     7561 tgatgcagac cgaaagctcc gttgagcaat acggcggcgc ggccctgctc gtgggcggct
     7621 atccaggtgc ctccggcccc catatcctga ggaaacagga ggcccggcct gcgttggatg
     7681 ccgtgcgcgg cgcggtgggg cgcttcatcc cggttggaac tgagcagcca ttcgtggtct
     7741 gggcctttcg gcaggtaggt gacggtacac attttcagta ggcggtgtgc ggttggcggt
     7801 gtgcagtaag cggtatgcgg tgtgcagtat gcagtaggcg gtgtgcggtt atttgacgcc
     7861 aaatttttga ggattattca tcatatagcc tatgagattt ccaacggatt gattgttggc
     7921 aaccaagtct tcataggtgt ttttgtcaat atattcgcat tccagtgcaa actccaacca
     7981 cgccaaagtg tctgcgtttt cagcgtcgct atcggaaagt ttgctcgcat agtgcgccgg
     8041 atactgcctt tttctgtacg cttccgcaat gttggcacac acagattgcg aacttctaca
     8101 tatctgatct ataagggagt aggtctcttc tttaggaaat ttcttactca ccttgaaaat
     8161 ctccatggct aactcaaaac caagtttgta agcgtgcaaa tctctaaagt ccatcttcct
     8221 tgtatttttt ttagttaaaa attactgcct actgcccacc gcctaccgcc cactgcccac
     8281 tgcctaccgc ctaccgcttc aaatgtcatc ccgcgtccag gagttgttga ccaagcgcca
     8341 gatgccgcgc aggtttttgt ttttgagttc gtcgggcagc agttcgtcgc tgaagttttg
     8401 gaacgaaacc ggcctcaccc aacgacgaac ggcgtggatg cccactgcgg tgaagcggct
     8461 gtcggtgctg gccgggaaag ggccgccgtg cgtcatggca ggcgctacgg ccacgccggt
     8521 gggcacgccg ttgaggacga tgcggccggc tttgcggcgg gcnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnngccaat tgggcggcgt ctttgcagat caccatgagc gaaaacggcc
     8701 cgaagacctc ttcgtgcagg tgtggatttt gtaaaaagtc gtcgcccgac acccaggcta
     8761 cggctgccga gggcgcgcct gcgtcggact gcccggcgag atatacgcct ttgctgccga
     8821 gggtttgggc caatttttcc acatagtttc ggctgatgcc ctcgtgcagc atgggagccg
     8881 gtttggcttg cgcaatatgt tgtcccagca cctcgatgaa atgccgcagc cccatgcaat
     8941 ctatgcccat catcaggccc ggattggtac aaaactgccc catgcccagc gtgatggaac
     9001 ctgcgcaagt ctgcgcgatg gcctctcccc gcttcgatac ggcttcggtc aaaacgagca
     9061 tcggattgac actgcccatc tcggcaaaaa cggggatggg gtcggggcgt tgctgtgcat
     9121 agccgtgcag cgccatgccg cccgacagcg agccggtgaa gcccaccgcg cgggtttgcg
     9181 ggtgcatgac caaatatttt ccggtgtcaa atccctcatc ttcaaggtgt tgaaatacgc
     9241 cggtcggaac atcacaggta tcggctgccc gatcaatggc cccggccacc agcattgaag
     9301 tgcgcgggtg ggcaggatgc gccttcacta ctacggggca accggcagcc agcgcagagg
     9361 ccgtgtcgcc gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcggcct
     9481 ctacccagga gccttcgcgc agcatggccg cgtacatgcg cagttggccg gtggtgcgca
     9541 tgcgttcgcc ggtgaggcgg gcttcgtcca agttggtttc ctgcatggcc gtttgaatca
     9601 ggatactgtc cagcgcttcc atttcggtgg cgatggtctc gagaaagcgg gcgcgcagtt
     9661 cgggggccga ttggctccag gcttcggctg ccgcgccggc gcgttgcatg gttatgttga
     9721 ctttttcctg tatggtcatt ttttttttgg ttggttg
//
LOCUS       DAORLF010000145         9639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3805_length_9639_cov_2.248957, whole genome shotgun sequence.
ACCESSION   DAORLF010000145 DAORLF010000000
VERSION     DAORLF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_9639_cov_2.248957"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..623
                     /locus_tag="PK198_12625"
     CDS             153..623
                     /locus_tag="PK198_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF39625.1"
                     /translation="MSLKTGDAAPLFTLYSSDKQPVSLENYRGKNVVLLFFPMAFTGV
                     CTAELCSMRDSIADYAGLDAEILAISVDSPFTLDKFKQDQQLNFPLLSDFNKEVSAAY
                     GTIYEEFVFGLKGVSKRSAFVVDKEGVIRYAEVLESAGNLPDFEAVKAALAELN"
     gene            714..1010
                     /locus_tag="PK198_12630"
     CDS             714..1010
                     /locus_tag="PK198_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRF39626.1"
                     /translation="MRVFSGFEEELEDLLVEDEIADTEGPAFLVVYNDDHNSFDWVIR
                     CFMEVLDHSHEQSEQLAIMIHFKGKATVKTAPKKELKPKKDALVERGLSAVIEE"
     gene            complement(1207..>1803)
                     /locus_tag="PK198_12635"
     CDS             complement(1207..>1803)
                     /locus_tag="PK198_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF39627.1"
                     /translation="IRGGSEMGEQWYEDGKLMKKKNTFTDFIACAEELVKQKYTSPKR
                     LAINGGSAGGLLMGVVVNMRPDLFQAVVAQVPFVDVINTMLDPTLPLTTQEYEQWGNP
                     NEKEAYDYIRSYSPYDNIEKKDYPHILATGGLNDSQVLFHEPAKWVAKLRATKTDNNI
                     TLLRTNMKSGHGGATGRFDRLKEVAFNYAFMIDRLGVK"
     assembly_gap    1804..1837
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1838..3361)
                     /locus_tag="PK198_12640"
     CDS             complement(<1838..3361)
                     /locus_tag="PK198_12640"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766919.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HRF39628.1"
                     /translation="MQKMLLFALALAMAAGFSACNNQPTETTETTMRMQSDYPTPPAA
                     AVQPETFSEHGYERVDNYYWLKDKTNPEVIAYLNAENAYADTVMAHTKDLQETLFNEL
                     KGRIKEDDSSVPYFDNGYYYYSRTETGKQYRIYCRKKGSVDAPEEIILDENKEAEGQQ
                     AFVLAAFEVSPDNRILAYASNYTGSYVEFTLRFRDLTTGKDLPDVIENAGGGAWANDN
                     TTVFYTRPNAALRTDKVFKHKLGTKVKDQLVFEEKDELFSLDVSRSKSGDYIFLTSGS
                     FTSTEYHLLPSSQPDGKFQVFAPRAKDVEYYVSHHKQKFIIRLKDDNHKNYKIMEAPL
                     TGFSDKKNWKDLIPHDPKVMIEDMEVFDKFLAIEVRTNGLQELRVMDLQSGAMQSVTF
                     PEPVYTVFSSSNADYASTKLRYGYMSLNRPSSVYDYDMTTGKSELLKQQEIPSGFNPD
                     DYEVKRLWATAPDGVKVPMALVHKKGMKLDGNNPTLLYSYGSYGSSTDPYFNGNAFSL
                     "
     gene            3678..4256
                     /locus_tag="PK198_12645"
     CDS             3678..4256
                     /locus_tag="PK198_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39629.1"
                     /translation="MPHEVEIQDLKMLLAELIASQKDTEARFKETEARFKETADRFKD
                     TDKRINKAFELFESQWGKLIEALVEGDLVRVLNERGIAVAETATRRKGRRGGHNYEFD
                     IIAHDTTQIVVVEVKTTLRVKHVEHFLEKMSHAKEWMAEYKNYEVLGAVAFLLAEEAA
                     DTLAEKKGLFVICATGDSAAIVNADDFQPRRF"
     gene            4273..5040
                     /locus_tag="PK198_12650"
     CDS             4273..5040
                     /locus_tag="PK198_12650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF39630.1"
                     /translation="MKQTLTLFLLLHIWAVAAQPAFRFSYGAIVRGDTTSPRMAVVFT
                     GHEFADGGNFIANTLKKRNLKASFFLTGDFYRNPDFGKLIGQLRQNGHYLGAHSDAHL
                     LYCDWSRRDSLLVTQKQFVDDLTANYTAMFPFSIPPAEDELYFLPPYEWYNDTIAVWT
                     RQQGLQLINFSPGTRSAADYTWPQLPNYRSSRVILQSIRDFERASPAGLNGFILLLHI
                     GTDPRRTDKFYKKLPTLLRFLKARGYQPVRVDELLNR"
     gene            5130..>5366
                     /locus_tag="PK198_12655"
     CDS             5130..>5366
                     /locus_tag="PK198_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39631.1"
                     /translation="MFRLITAAIATLFFLSLQAQTFPRFSVPFRTADGQLLRNALAGG
                     LNNPQVSEADLNNDGITDLFVFDRKGNVILPFLHD"
     assembly_gap    5367..5376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5377..7402
                     /locus_tag="PK198_12660"
     CDS             <5377..7402
                     /locus_tag="PK198_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39632.1"
                     /translation="AGSVHYTYAPEYADNFPALNSWALLRDFNGDGIADLFAYSDAPV
                     DGIMVYRGYFDAQNRLSFQRLMFNNLHRLIYFPLPGGSTTQLYVSRIDIPEITDVDGD
                     GDLDILTFGVNGGKMEYYANRSLEMGMGRDTVVYRLVSNCWGGFFESGFSEHIDLAPN
                     FGQCYNPFHDGSGEGLELRHVGSTVLAFDADGDGDKDLILGDVSFNNLNFLTNGGTPQ
                     TAWMTAQDIDFPSYDVPVDLPLFPAAFLVDVDHDGKKDLLVAPNAAVGSEDYHALWWY
                     RNTQGIPHRFERQQTDFLIETMLDFGTGAYPAFADVNGDGLTDLVVGNNSYYREFGAK
                     ESRLHLFLNTGTPEVPAFQLADDNWLNMRAIAAATFNLAPAFGDLDGDGDTDLLVGTD
                     LGQLYYFKNLAGANQPMQFGAHQFPYMNIDIGTASVPFMADLNRDGLMDILVGERNGN
                     INFFPNTGSGTQPDFVGDPDLEPNVFFLGGVDTRITGAFPNGYSAPAVVWNGQEYLLF
                     SGTSRGRIEAYTVLPSSFRDSFPLISETWGEIHTGTQSRLAFVDLDNDGLLELAVGND
                     RGGLQFFRTPLPALAPNSVKPEPAAGPSVKVFPNPTHNTLFFSRTDAGTAELELSIYN
                     AEGRLMRQSRRSGAHWQEDLGNWPDGMYFWRLAGTDGVISAKVMKTAKGR"
     assembly_gap    7599..7672
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7673..>9639)
                     /locus_tag="PK198_12665"
     CDS             complement(<7673..>9639)
                     /locus_tag="PK198_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRF39633.1"
                     /translation="VSIGSMVDVGFAELIDYFGMDNNTSSILIYMETLKDARKFMSAA
                     RAFARSKPIIILKAGKSQEGGKATMSHTGSVAGNDAVFDAAFQRAGIIRVDTIAQLFH
                     CAQALAMQPRPQGNRLAIVTNAGGPGVLATDYLATRGGKLAELSEQTMQKLNEFLPAS
                     WSHGNPVDVLGDASAETYRKTLEICQADENVDGVLTILTTQAVTSPTDAAREVVEASK
                     NTRKPMLAVWMGEADVTEGREVLEAGKIPNYRYPESAVDVFLKMWNYSRNLDLLYETP
                     EEAPQEFTPRKDSAWRIIRRALSENRRQLTEIEAKELMSCYAIPVGENRLATRPEEAA
                     DMAEAVGFPVAMKIVSPDALHKTDVGGVKLNISDRAAATATYNEIVSSVLSHVPKANI
                     HGVLIERMTKKPFELLIGAKKDPIFGPAIAFGRGGVAVEVYKDTQLGLPPLNMRLARR
                     VIEGTQMYPLLKGYRNMPGVDLEDLAFVLTKFSHLLTDFPEIGEIDINPYTADAESGL
                     VLDARIILDEYKPRRKGHPYDHLVISPYPGKYTKRVLLKNGVEAVLRPIRPEDEPMET
                     QMIENLSSETLYFRFFGYVPKVNHDFLTRFTHIDYDREMAIVAEVEEGGNKKMVGVVR
                     IIADAWGESAEYAIVVADEWQGLELGGNMMD"
BASE COUNT         2037 a         2668 c         2467 g         2349 t
ORIGIN      
        1 ccgctaaatc tgttcccaca ctatttgtca aagaaggatt ttttttagga ctgcaaagca
       61 aagcaatatt tggcgcaggc agaaaacatt cctttgggcg gtgtgttctt tgggtgaaat
      121 ttttcctgtc actatacaaa aacgaaaata ccatgtcact gaaaaccggc gatgccgctc
      181 cgctttttac cctctattct tctgacaagc agcccgtttc gctcgaaaac taccgaggca
      241 aaaacgtggt gctcttattt tttccgatgg ctttcaccgg cgtttgtacg gccgaattgt
      301 gcagcatgcg cgacagcatt gccgactatg ccggcttaga cgctgagatt ttggccattt
      361 ccgtggattc gccgttcaca ttagacaagt tcaaacaaga tcaacagctc aactttccgc
      421 tgctgtccga cttcaacaag gaggtgtcgg ctgcttatgg cactatttat gaggagtttg
      481 tgttcggttt gaaaggcgtt tccaaacgct ctgcttttgt ggtggacaaa gagggcgtca
      541 ttcgttacgc cgaagtgttg gaatcggcgg gcaacctgcc ggacttcgag gcggtgaagg
      601 ctgcattggc tgaactgaat tgattgaaaa tatacttgat gaatctgtga gttgcgaaat
      661 tcaatcgcag ctcacagatt gctgcccata agtcaccgaa aaatagtcag gccatgcgcg
      721 ttttcagtgg attcgaggaa gaattggaag acctgttggt agaggatgaa attgccgata
      781 ccgaggggcc tgcttttttg gtggtgtata acgacgacca caattccttc gactgggtga
      841 ttcgctgctt catggaggtg cttgatcatt cgcacgagca gtcggaacag ttggccatta
      901 tgattcattt caaaggcaag gccaccgtaa aaacggcccc caaaaaagaa ctgaaaccta
      961 aaaaagacgc tctggtggaa cgcgggcttt cggcagtgat agaggagtga aaaccatttt
     1021 cagtctttcc gttccccggc tttttcctgt gcgtttggtt ttaaaacaca ataggcacat
     1081 agatcacata gcgttggtac tatgttttct atgtgcctat ctgctttata aacagctatt
     1141 tgtatctaag atttgctttg gacttcgcca aagtgcagcg atcaactgcc gcaggcagct
     1201 taccccttac ttcacgccta atcggtctat cataaacgca tagttgaagg ccacctcctt
     1261 cagccggtcg aaacggcctg tagcgccgcc gtgccccgac ttcatgttcg tgcgcagcag
     1321 cgtgatgttg ttgtccgttt tcgtggcgcg cagtttggcc acccatttgg ccggctcgtg
     1381 gaagagcacc tgcgagtcgt tgaggccgcc ggtagccaga atgtgcggat agtctttttt
     1441 ctcgatattg tcgtagggcg agtaggagcg aatgtagtca taagcctcct tttcattggg
     1501 attgccccac tgctcgtact cctgcgtggt gagcggcaag gtcggatcga gcatggtgtt
     1561 gatcacatcc acaaagggca cctgtgccac caccgcctga aacagatcgg ggcgcatgtt
     1621 taccaccacg cccatcagca ggccgcccgc gctgccgccg ttgatggcca agcgcttcgg
     1681 cgaggtatat ttttgcttca ccaactcctc tgcgcaggct atgaagtccg taaaagtatt
     1741 tttcttcttc atcagcttgc cgtcttcata ccactgctct cccatttcgc tgcccccgcg
     1801 tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa gctgaatgcg ttgccgttaa
     1861 agtaggggtc ggtactggaa ccatacgagc cataagaata cagcagcgtg gggttgttgc
     1921 cgtccaattt catgcccttc ttatgcacca gagccatcgg tactttcacg ccgtccggag
     1981 cggtggccca caggcgtttc acctcataat cgtcgggatt gaagccgctg ggtatttcct
     2041 gctgtttcag cagttcgctc ttgcccgtcg tcatgtcgta gtcatacacc gagctggggc
     2101 gattgagcga catgtagccg tagcgcagtt tggtagaagc atagtcggca ttagaactcg
     2161 aaaaaactgt gtaaaccggc tccgggaaag tcaccgactg catggcgccg ctttggaggt
     2221 ccatcacgcg gagttcctgc aggccgttgg tgcgcacctc tatcgccaga aacttgtcaa
     2281 acacctccat gtcttctatc atcaccttgg gatcgtgcgg tatcaggtct ttccagtttt
     2341 tcttgtccga gaagccggtg agcggcgcct ccatgatttt gtaattcttg tggttgtcgt
     2401 cctttagcct gatgatgaat ttctgcttgt ggtgcgacac atagtactcc acatctttgg
     2461 ctctgggcgc aaacacctga aacttcccgt ccggctggct gctgggcagc aggtggtatt
     2521 ctgtagaagt gaagctcccg gaagtcagga agatataatc gcccgatttg ctgcgcgaca
     2581 catccaacga aaacaattcg tccttttctt caaacaccaa ctggtctttt accttcgtgc
     2641 cgagcttgtg tttgaacact ttatcggtgc gtagcgcggc attggggcgc gtataaaaca
     2701 cagtggtatt gtcattggcc caggcgcctc cgcccgcatt ttcaatcaca tcgggcaggt
     2761 ctttgccggt tgtcagatcg cggaaacgca gcgtaaattc cacatacgag ccggtgtagt
     2821 tgctcgcgta ggccaaaatg cggttgtcgg gactgacctc aaaggcggcc agcacaaaag
     2881 cctgctggcc ctcagcttcc ttgttttcat ccaaaatgat ttcctccggc gcatccacgc
     2941 tgcctttttt gcggcaataa atgcggtact gcttacccgt ttcggtgcgc gagtagtagt
     3001 agtagccgtt gtcgaaataa ggcacgctgc tgtcgtcctc tttgatgcgg cctttcagtt
     3061 cgttgaacag cgtctcctgc aagtctttgg tatgcgccat cactgtatcg gcgtaggcat
     3121 tttccgcatt cagataggcg attacctccg ggttggtctt gtccttgagc cagtagtagt
     3181 tgtccacgcg ttcgtagcca tgttcgctga atgtttccgg ttgcacagcc gcggcgggcg
     3241 gagtcgggta gtcgctttgc atgcgcatag tcgtttcggt cgtttcggtt ggttgattgt
     3301 tgcaggcgct gaagccggcg gccattgcca aagcaagggc aaaaagaagc attttttgca
     3361 taggaaggtg atttttaagg tgcgaagtta agaataacct tgtcaaggtg atgtcttaca
     3421 taaaaaggtt gcatacttct ttgggcgcat ccccgccgct ctttatgctc ccgccatccg
     3481 ggctacaaaa aagggcgggg tcgctgcctt ccggggtttc gccccgctct tgcagaagcg
     3541 gggccgcctc ctcgcgtcgg cgccctccag gcagcttgcg cgggcgcctc cctccgcttg
     3601 acaatgcact gaaaatcatt taactttgct gccggatatt ttaccctgta tttcattcaa
     3661 aaaaatcatc cattacaatg ccccacgaag tagaaattca agacctgaaa atgctcctcg
     3721 ccgagctgat tgccagccag aaagacacgg aagcgaggtt taaggaaacg gaagcgaggt
     3781 tcaaagagac agccgaccga ttcaaagata ctgacaagcg catcaacaaa gccttcgaac
     3841 tattcgaatc gcagtggggc aagctcattg aggcattggt agaaggcgat ttggtgcgcg
     3901 tactcaatga gcgcggcatc gcagtagccg aaaccgccac ccgtcgcaaa ggcaggcgcg
     3961 gcggccacaa ttacgaattc gatatcattg ctcacgatac cacccaaatc gtggtagtag
     4021 aggtaaaaac aactctgcgc gtgaagcatg tggagcattt tctcgaaaaa atgtcgcacg
     4081 ccaaagagtg gatggccgag tacaaaaatt acgaagtact gggcgccgtg gccttcctcc
     4141 ttgccgaaga agccgccgac acccttgccg aaaaaaaggg cctcttcgtc atttgcgcta
     4201 ccggcgacag cgccgccatc gtcaatgccg acgacttcca gcctcgccgt ttctaataca
     4261 tcaacttacc ctatgaaaca aaccctcacg ctttttcttt tgctccatat ttgggccgtg
     4321 gccgcccagc ccgcttttcg ttttagctac ggcgccatcg ttcgcggcga tacgacttcg
     4381 ccccgcatgg ccgtcgtttt taccggccac gaatttgcag acggcggcaa ttttatcgct
     4441 aacactttga aaaagagaaa cctgaaggcc tccttttttc tcaccggaga tttttaccga
     4501 aaccccgatt ttggaaaact catcggccaa ctccgacaga acggccacta cctcggcgcc
     4561 cacagcgacg cgcacctgct gtactgcgat tggagccgcc gcgacagcct gctcgttaca
     4621 cagaagcaat tcgttgacga tttgaccgcc aattacacgg ccatgtttcc attcagtatc
     4681 cctccggcgg aggatgagct ttattttctg ccgccttacg aatggtacaa cgacaccatc
     4741 gccgtttgga cccgccaaca gggcctccaa ctcatcaatt tcagtccagg cacccgcagc
     4801 gccgccgatt acacctggcc gcaattgccc aactaccgca gcagccgcgt cattctgcaa
     4861 tctattcgcg attttgagcg cgcctcgcct gccggtctca atggctttat cctgctgctg
     4921 cacatcggta cagacccccg ccgcacagac aagttttaca aaaaactccc gactctcctg
     4981 cgctttctga aagcacgggg ctaccagcct gtccgcgtgg acgaactgtt gaacagatga
     5041 tttagccgcc tgccgtcgtt tttgcatttt cccggcatcc aagcccccca aaacagcgtt
     5101 ctttgtgcag tgtttttaat catactacca tgtttcgact cattactgcc gccatagcaa
     5161 cgctcttttt cctttcattg caggcgcaaa ccttcccgcg attttccgtc ccgtttcgca
     5221 ccgccgatgg ccaattattg cgcaacgccc tcgcaggcgg cctcaacaac ccacaagttt
     5281 cggaggccga tctgaacaac gacggcatca ccgatctgtt tgtatttgac cgaaaaggta
     5341 atgtcatcct gccgtttctg cacgacnnnn nnnnnnggca ggctccgtcc attataccta
     5401 cgccccggaa tacgccgaca attttcccgc tctcaacagt tgggcgctgc tgcgcgattt
     5461 caacggcgac ggtattgctg acctcttcgc ctattcagat gcgcctgttg acggcattat
     5521 ggtgtatcga ggctatttcg atgcgcaaaa ccgcctgtct ttccagcgcc tgatgttcaa
     5581 caatctccac cggctcattt attttcccct gcccggcggc tctactactc aactctatgt
     5641 atcccgaata gacatccccg aaatcacaga tgtggatggc gatggcgacc tcgatatcct
     5701 cactttcggc gtgaacggcg gcaaaatgga gtattatgcc aatcgctcct tagagatggg
     5761 catgggccgc gataccgtcg tgtatcgctt agtgagcaac tgctggggcg gtttttttga
     5821 aagcggcttc tccgaacaca ttgacttggc ccccaacttc gggcaatgct acaatccctt
     5881 ccatgacggc agcggtgaag gactcgaact ccgccatgta ggctctacgg tgcttgcatt
     5941 tgatgccgac ggcgacggcg ataaagacct catactgggc gatgtatctt tcaacaacct
     6001 caactttctc accaatggcg gtaccccgca aaccgcctgg atgaccgctc aggatattga
     6061 cttcccctcc tatgatgtgc cggttgatct gccgctgttt cccgcagcct tcctcgtgga
     6121 cgtggaccac gatggcaaaa aagacctgct cgtggcgccc aatgccgcag tcggttcgga
     6181 agactaccac gccctgtggt ggtaccgcaa tacgcagggc attccccacc gcttcgagcg
     6241 gcagcaaacc gatttcctca tagaaaccat gctcgatttc ggcactggcg cttatcccgc
     6301 tttcgcagat gtgaacggcg acggcctgac cgacctcgtg gtgggcaaca attcttacta
     6361 ccgggagttc ggggccaaag aatctcgcct ccatctcttc ctcaacaccg gcacgcccga
     6421 agtgcccgcc ttccaattgg ccgacgacaa ctggctcaat atgcgcgcca tagccgccgc
     6481 taccttcaac ctggcgcctg ccttcggcga tctggacggc gacggcgata ccgatttgct
     6541 ggtgggtacc gacctggggc aactctatta ttttaaaaac ctggccgggg caaaccagcc
     6601 catgcagttt ggcgcccatc agtttccgta catgaacatt gacatcggaa cagcctctgt
     6661 gccttttatg gccgatctga atagagatgg cctcatggac atcctcgtgg gcgaacgcaa
     6721 cggcaacatc aattttttcc ccaataccgg ctccggcact cagccggatt tcgtgggcga
     6781 ccccgatttg gagcctaatg tattttttct ggggggggtg gatacccgta ttaccggcgc
     6841 ttttcctaac ggttacagtg ctcccgcagt agtatggaat ggtcaagagt acttactttt
     6901 ctcgggaacc agccggggga ggatcgaggc ttataccgta ctcccgtcga gctttagaga
     6961 ttcttttcct cttatttcag aaacctgggg tgaaattcat acaggtactc aaagccgcct
     7021 cgctttcgtc gatttggaca acgacggcct gctggaactg gcggtgggca acgaccgggg
     7081 cgggctgcaa tttttccgaa cgccgctgcc tgctctcgca cccaactctg tgaaaccgga
     7141 acctgccgcc gggccaagcg taaaagtctt tcccaacccg actcacaata ccttgttttt
     7201 cagcagaacc gatgccggaa cagcagaact ggaactcagc atttataacg cagaaggccg
     7261 cctgatgcgg caatctcgca gatcgggcgc tcactggcag gaagacctcg gcaactggcc
     7321 cgacggtatg tacttctggc gcttggccgg caccgatggc gtgatttcag ccaaggtgat
     7381 gaaaacggca aaggggcgat aaaaaaataa agtggtcatt ttcgccccac tacaaagtga
     7441 tgatccgggc gacaagctga aaaatttgtc gcccggcatc tgctttctta caccggctgg
     7501 aaaggcagct cggcggcaaa cggaatggct gtttccaatt ccagttccgc gcggtaggtt
     7561 tcaaagtctt cccgactgat cttgaagccc cgcttttcnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagtccatc
     7681 atattacctc ccaattcgag gccctgccac tcgtcggcta ccacgatggc atactctgca
     7741 ctttctcccc aggcatcggc aatgatgcgc accacgccca ccattttctt gttgccgccc
     7801 tcttccactt ccgccacgat ggccatttcg cggtcgtagt caatatgtgt aaagcgcgtg
     7861 aggaaatcgt ggttcacctt cggcacatac ccaaagaagc ggaagtataa ggtttcgctc
     7921 gacaagttct caatcatctg ggtttccatc ggctcgtctt cggggcggat gggccgcagg
     7981 acagcctcta cgccgttttt cagcagcacg cgcttggtgt atttgccggg gtacggcgat
     8041 atgaccaagt gatcgtacgg gtggcccttg cggcggggtt tgtattcatc cagaatgatg
     8101 cgtgcatcga gtaccagccc cgattcggca tcggctgtgt aggggttgat atctatttct
     8161 ccaatctccg ggaagtcggt gagcaggtgc gaaaacttgg taagcacaaa ggccaggtct
     8221 tccagatcca cgcccggcat attgcgatag cctttcagga gcgggtacat ctgcgtaccc
     8281 tcaatgacgc gacgcgccag gcgcatgttc agcggaggca atcccaactg cgtatcttta
     8341 tacacctcga ccgccacgcc gccccgacca aatgcaatgg ccggcccgaa aatggggtct
     8401 tttttggcgc cgatgagcag ctcaaatggc ttcttcgtca tccgttcgat gagtacgccg
     8461 tgtatattcg cctttggcac atggcttagt accgagctga cgatctcgtt gtaggttgcg
     8521 gtggctgctg cccggtcgga gatgttgagt ttcacgccgc ccacatcggt tttgtggagc
     8581 gcatcggggg aaacgatttt catggccacc ggaaaaccaa cggcttcggc catgtcggcg
     8641 gcttcttctg ggcgggtggc cagccggttt tcgcctaccg gaatggcata acaggacatc
     8701 agttctttgg cctcgatttc ggtgagttgc ctgcgatttt ccgataaagc tctgcggata
     8761 atgcgccagg ctgagtcttt gcgcggggtg aactcctgcg gtgcttcttc gggcgtttcg
     8821 tagagcaaat ctaagttacg agagtagttc cacattttca ggaacacgtc cacggcgctt
     8881 tccgggtaac ggtagttggg aattttgcct gcttccagca cttcgcgtcc ctctgtcaca
     8941 tcggcctctc ccatccacac ggcaagcatt ggtttgcgcg tatttttgga ggcttccacc
     9001 acttcgcggg cggcatcggt gggactggtc acggcctggg tggtgaggat ggtgagcacg
     9061 ccgtccacat tttcatcggc ctgacagatt tcgagcgttt tgcgataggt ttctgcactg
     9121 gcgtcgccga gcacgtccac cgggttgccg tggctccaac tggcaggcag aaattcgttg
     9181 agtttttgca tggtttgctc cgacaactct gccaatttac ctccacgtgt agcgaggtag
     9241 tcggtagcca atacgccggg gccgccggca ttggtcacga tggccaagcg attgccctgg
     9301 ggccggggct gcatggccaa agcctgcgca cagtggaaga gctgggcgat ggtgtccacc
     9361 cggatgatgc cggcgcgttg gaacgcagca tcgaatacgg cgtcgttgcc ggccactgat
     9421 ccggtgtgcg acatggtagc cttgccgcct tcctggctct tgcccgcctt taggatgatg
     9481 atgggtttgg agcgggcaaa agcgcgggcg gcgctcataa atttgcgcgc atctttcagg
     9541 gtttccatat aaataaggat ggacgaggtg ttgttgtcca taccaaagta gtcaatcaac
     9601 tcggcaaaac ccacgtccac cattgaaccg atggaaacg
//
LOCUS       DAORLF010000146         9555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3849_length_9555_cov_2.309053, whole genome shotgun sequence.
ACCESSION   DAORLF010000146 DAORLF010000000
VERSION     DAORLF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_9555_cov_2.309053"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="nadE"
                     /locus_tag="PK198_12670"
     CDS             complement(<1..1198)
                     /gene="nadE"
                     /locus_tag="PK198_12670"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRF39634.1"
                     /translation="MKIALAQINAHIGHFEGNVARMLAAVEEAKLQGADIVVFPELST
                     CGYPPRDFLEFDDFVHLADAAVQRLAEAARGIAILVGSPTRNPVPEGKDLYNSAYFLA
                     EGAVRFVQHKTLLPTYDVFDEYRYFEPATQFRVLEFKGKRIAVTICEDIWNIGNENPL
                     YTVCPLDEMAPLQPDLIINLSASPFNYAHSHGRISVVKANVERYGIPMFYVNQVGAQT
                     ELIFDGGSMVARPGGKIFDELPYFEECVRTYDLNEVLTQPGPGEQPKEKYRLIHDALL
                     LGLRDYFGKLGLRQAILGLSGGIDSAVTAVLAARALGPENVRGLLLPSQFSSGHSVDD
                     ARQLTENLGMRYDVVPIGPAYDSFLQTLEPFFEGRPFDLAEENLQARIRAVLLMGFSN
                     KFGPILL"
     gene            complement(1198..1905)
                     /locus_tag="PK198_12675"
     CDS             complement(1198..1905)
                     /locus_tag="PK198_12675"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF39635.1"
                     /translation="MRKYFPASVAALLFFTLIILSGCRQESAPALPPPSADNTDQAAE
                     SPARKPGIAEDYLNTNRVIWQKPDLVLQQLGDLKDKTVADIGAGTGFFSLRMATVAQK
                     VVAIDIDPRFVSHLDSLKKLELPADIQSRLETRLAKPNDPNLRPGEADVIVIVNTYMY
                     IKDRVGYLRNLKNGLSDGGRLLIVDFKKKRMPLGPPQDIRVPLFQAEDELYEAGYRDI
                     ATNDTSLDYQYIITARK"
     gene            complement(2122..3477)
                     /locus_tag="PK198_12680"
     CDS             complement(2122..3477)
                     /locus_tag="PK198_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39636.1"
                     /translation="MLLRLSFITNILTVLGFLFAGSHALQAQALPGSPKPYRVVFQAN
                     NEWQGPLVNNRRLTHTYDAQQRLSQSVKEVWSESNQQFAVSERTEYDYADDGRLREIR
                     NGYWNATSAVWQNTSRERYYYHSGLLDSLWTLEYFNGQSWSIAQRRRTSYTTDLQILS
                     QREENRSGNAWIVRTEILNTYNTQGKRIENLRHFNSETGVPQTSYRYRYEYDTAGRLE
                     SEEYEAVVYSISNDWAKQHRDEYFYTDADERVDERRRFGWFDLQQQWGLVSVIAYTYT
                     PTETIELGDATASHPSYRRSTRYNAVGQLTAMTFENWNNALGSLNPINESTWAYRPDG
                     ALLEFRSFYAEINAPNLLLYPGTAELYEYDATTAVRESWKDWEAIVFPNPATDAVSVT
                     SRGAAIQQIVLLDAAGKALRTIPVATPIAHIERGTLPAGVYTLLLHSEAGVTARRVVW
                     R"
     gene            complement(3671..5665)
                     /locus_tag="PK198_12685"
     CDS             complement(3671..5665)
                     /locus_tag="PK198_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39637.1"
                     /translation="MKKIMLVFSFLLCLLANISAQSPQQFKYQAVARDASGQPYASTP
                     LRLRFFIAQSNANGPAVYAEEHTVTTTSLGVFDLNIGSGTPIGGGSLANVDWSAGPYF
                     LRVELNPNANGGAFIPMGTSQLLSVPYALYAESSGSDRDEQTLSVNGTQLSISGGNTV
                     SLPAGPQGPTGPAGPQGATGPMGPQGPTGPTGAAGPQGLPGIAGPQGATGPIGPQGPA
                     GPMGATGPQGPAGNYTAGSGISISNAVISAADNSPVNELQTLSLNGNVLSISGANGSG
                     THGATVTLPSGGGSNYLGGSGISIVPGFLGDFIINNAAPSKWTENGSNIYNTNGGTVT
                     IGTTTPSGHSQLTVVGDHPNTGYGITGSGTSTGVAGYGGAAGVAGGSENGAAVLGISN
                     FGYGGFFSSPFGGHALITEQGNVGIGTNSPQYKLDVWGTARIVDELTVNSDITCGNIF
                     PAADNAYDLGDFGFRWRSMHTGELRAIVTTAPQTAIIGMNTDPNGYAAEFFGDVEVIG
                     NLAKSAGTFKIDHPLAPADKYLYHSFVESPDMMNIYNGNATTDAAGMATVELPAYFEA
                     LNMEFRYQLTCIGQFAQAIVKEKVAGNRFVIQTDKPGVEVSWQVTGIRQDAYAKANRV
                     VPEVEKKADDKGKYLHPELFGAKPEDRIGSHRATGAASKE"
     gene            complement(5704..6192)
                     /locus_tag="PK198_12690"
     CDS             complement(5704..6192)
                     /locus_tag="PK198_12690"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39638.1"
                     /translation="MKSLRLCVAWLCLAFSAAASGQSLGPVLLGSAGAQAENSSAGSL
                     YWSVGEPATATYSQNAAMLTQGFHQVFVTVSTAVESPAELLTEVRAFPNPSTDRLYIH
                     SETPVQIRLLHLHGIEVFASAQPDHHHELKTAALPAGLYLLEARDPETRRFKTFKIVI
                     TH"
     gene            complement(6283..6999)
                     /locus_tag="PK198_12695"
     CDS             complement(6283..6999)
                     /locus_tag="PK198_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF39639.1"
                     /translation="MKTTLNFAFRAGIILALFFTMIASVVAQKTALPCHCPAPVNSQV
                     LYLSSVSYAITWTPPADVSAVNGYTVRTTLLPAGTVVSEDIVAGPPYVKTGLSPNSRY
                     RIEVFSRCRCGLSTSLSTIIDTPVGIIMEDIIVQRTSPNNLGPAKETSNLFIEWSGSG
                     TQEYVKVISGGSHIWIKKGADDVIALVNSAGLFGVIDNTPVYHSNIVVRSNTAGTIAT
                     LHLTTSGVLVESGNVILERY"
     gene            7160..8815
                     /locus_tag="PK198_12700"
     CDS             7160..8815
                     /locus_tag="PK198_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39640.1"
                     /translation="MKGSTLLLTLRLLTEEERAALELMAASPLFKLGNRHQDAVELLQ
                     YLRPFAPQYESLEMDRQVAAQALFGHRTQPEAELRKAMSNLNTLVRKLILLQQTLTDD
                     DGPDAQLTAVRTDLALMRFCNERLDLSAAKKKSSANVSGRKAKQTDDLVAQTYNSITA
                     KTQCLQTEAAQTFTARQYLEMRNIRLEAAHEWHVHHLFNGSTDVRQLLDIVLELEQLY
                     HYQRLDRQVSLRVSLLGMNTLQEEDSVRAALHYLEVVGSRKPVLPVLSDTPIELELYR
                     QALIMLQSFDAPESTAAFEYLDNQLKNKQLPLPAEQIKGLKAVLRFYCGIMANRRQDG
                     YFLQKRFELFQEHIAEEMLLNRGGVSAVQLCGLLSDALRLGDHNHAWAEDFLQQFENG
                     AQIAHTETPREVYKINKANLLFHQGRFRETANELIGYEWYGRVDNPQILLLAVRIDLK
                     ARYELGLYDDDHTLRTIDAAEKRILRLDDLDPQLARMTLFFLRTVRQLAGIRHRMSQP
                     LASGSRRFDAAGKLERLAGAIEEQPIAEKVWLKEQLAAAGSTG"
     gene            complement(8830..>9555)
                     /locus_tag="PK198_12705"
     CDS             complement(8830..>9555)
                     /locus_tag="PK198_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39641.1"
                     /translation="KLALLYRMKPHWSWRMLLAQAFRDPPVAERFVKFEQGGGLAFRP
                     NPDLRPERLVLSAELGTRIDIKKAGALDMALFYNRYKNLISFQQVSQPLEPLTYQVVN
                     LKESLMQGLEISWRRQWSEALSTSLSYTFLDARDISPERFNDALAYKVRHTLGGSATL
                     RHKGWMLHLDGRYRSRVEEVFIYPGSEPAAALIGNARLSRQIKERHCIYFSVNNFSNT
                     QYEELERYRMPGRSFAAGVELAF"
BASE COUNT         2137 a         2476 c         2785 g         2157 t
ORIGIN      
        1 tgagcaggat cggcccgaat ttattggaaa atcccatcag cagcaccgcc cggatgcgcg
       61 cctgaaggtt ttcttctgcc agatcgaaag gccgaccctc aaagaacggt tccagcgttt
      121 gtaaaaaact gtcataagcc gggccaatgg gcactacgtc atatcgcata cccagatttt
      181 cagtcaattg tcgggcgtcg tccaccgaat ggccggagga gaactgagaa ggcagcagca
      241 gtccgcgcac gttttcgggg ccgagcgcac gggcagccag tacagccgtc acagcagaat
      301 caatgccgcc ggagagaccg aggatggcct gtctgaggcc gagtttgcca aaataatcgc
      361 gcagacccag caacagtgca tcgtggatga ggcggtattt ttctttgggc tgttcgcctg
      421 ggccgggctg cgtgagcacc tcattcagat cataagtgcg cacacattcc tcaaaataag
      481 gcagttcgtc aaaaatcttt ccgccgggcc ttgctaccat cgagccgccg tcgaagataa
      541 gctcggtttg cgcacccacc tgattaacgt aaaacatggg gatgccgtag cgctccacat
      601 tggctttgac caccgaaatg cggccatgcg aatgtgcgta attgaacggc gaggccgaga
      661 ggttgatgat gagatcgggt tgcaggggcg ccatttcatc caaagggcaa accgtataaa
      721 gcgggttctc attgccgatg ttccagatgt cttcgcagat cgtgacggcg atgcgctttc
      781 ctttgaactc caacactctg aactgcgtag ccggctcgaa atagcggtat tcatcgaaca
      841 cgtcgtaggt gggcagcagg gttttgtgct gtacgaaacg cactgcaccc tcggccagaa
      901 aataagcgga attgtagagg tctttgccct ccggcaccgg attgcgcgtc ggcgatccca
      961 ccaaaatggc aatgcctcgc gcggcctcgg ccaggcgctg tacggcagca tcggccaagt
     1021 ggacgaagtc gtcgaattcg agaaaatccc ggggcggata cccgcaggta gagagttcag
     1081 gaaacaccac gatgtcggcg ccctgcaatt tagcctcctc tacggcggcg agcatacggg
     1141 ctacattgcc ctcaaaatgg ccgatgtggg cattgatttg cgccagtgcg attttcatta
     1201 ttttcgggcg gtgatgatgt actgataatc gagcgaagta tcattggtgg caatgtcgcg
     1261 gtagccggcc tcgtagagtt catcttcggc ctggaaaagc ggtacgcgaa tgtcctgcgg
     1321 cggccccagc ggcatacgtt ttttcttgaa gtccacgatg agcaggcggc ctccgtcgga
     1381 aagcccgttt ttgagattgc gcaggtagcc cacccggtct ttgatgtaca tgtaagtatt
     1441 gacgatgaca atcacatcgg cctcgccggg gcgaaggttg ggatcgttgg gcttggccaa
     1501 gcgggtttcg agtcgcgatt gaatatcggc gggcaattcc aattttttga ggctgtccaa
     1561 atgactgacg aagcgcgggt caatgtcaat ggccactacc ttttgggcaa cggtagccat
     1621 acggagcgag aaaaagccgg tgcctgcgcc gatgtcggcc acggttttgt ctttcagatc
     1681 gcccaattgc tgcaacacca gatcgggctt ttgccagatc acgcggttgg tattgaggta
     1741 gtcttctgca atgccgggct tgcgggccgg agattcagcg gcctgatcgg tgttatccgc
     1801 agagggcggg ggcagcgcgg gcgccgattc ttgccggcaa cccgacaaaa tgatgagagt
     1861 gaaaaagagc agcgcagcaa ccgacgccgg aaagtatttt cgcatacctg aaacctttta
     1921 aagcggatgt accaaagttg gtgtaaaagg gcgctaaaaa aatagatttt cattttcgcc
     1981 aaaggattcg ccaagtaaac gcttcatttc aagtgtcttg gcaaatcctt tggcctgaag
     2041 aaaatgaaaa tctatttttg cccactacaa aggtatgttg cccggcggaa tgttgggatg
     2101 tttagagggg atttttgtgt gttacctcca caccaccctt ctcgccgtca cgcctgcctc
     2161 gctgtgcagc aacagcgtat aaacaccggc gggcagtgtt ccccgctcga tgtgggcaat
     2221 tggggtggca acgggaatgg tgcgaagcgc cttgccggcg gcatcgagca acacaatttg
     2281 ctgaatcgct gcgccccgac tcgtcacgct gaccgcatct gtggccgggt tgggaaaaac
     2341 aattgcctcc caatctttcc agctttcgcg cacggcggta gttgcatcgt attcgtacaa
     2401 ctcggctgtg ccgggataga gcaggagatt cggagcgttg atttcagcat aaaaggagcg
     2461 aaattccagt agagcgccgt cggggcgata ggcccaggta ctctcgttga tgggattgag
     2521 gctgccgagg gcattgttcc agttttcaaa agtcatggcg gtcagttgtc cgacggcatt
     2581 gtagcgagtg ctgcggcggt agctggggtg cgaagcggtt gcatcgccca gttcgatggt
     2641 ttcagtgggc gtgtaggtgt aggctatgac cgaaacaagg ccccattgct gttgaagatc
     2701 gaaccatccg aatcggcggc gctcgtccac acgctcgtcg gcatcggtgt agaaatactc
     2761 gtcgcggtgc tgtttggccc agtcgttgct gatggaataa accaccgcct catactcttc
     2821 ggactccaag cggccggcgg tatcgtactc gtagcggtag cggtatgaag tttggggaac
     2881 gcctgtttcc gagttgaaat gccgcaggtt ttcgatgcgt ttaccctggg tattataagt
     2941 gttcaggatt tctgttctca caatccaggc gtttccgctg cggttttcct cgcgctgaga
     3001 aagtatctgg aggtctgttg tataggaggt tcggcggcgt tgggcgatgc tccagctctg
     3061 gccgttgaaa tattcgagcg tccacagcga gtcgagcagg ccggagtggt aatagtagcg
     3121 ctcccgcgat gtattttgcc agacggcgct ggttgcgttc cagtatccgt ttctgatctc
     3181 gcgcaggcgg ccatcgtcgg catagtcgta ttcagtgcgt tcggagacgg caaattgttg
     3241 attcgattcg ctccacacct ctttgacgga ttggctgagg cgttgctgcg cgtcataagt
     3301 gtgcgtcagg cggcggttgt tgaccaaagg tccctgccat tcgttgttgg cttgaaagac
     3361 aacccgatag ggcttgggcg agccgggcaa ggcttgagcc tgaagggcgt gcgagccggc
     3421 gaacaggaag ccgagcacgg tgaggatgtt ggtaataaaa gaaagacgta gtagcatgat
     3481 gtggatgttt tttttaagat ttaaggaagt aaaaccaaat gcccggcagc ccacaggtgg
     3541 agcgcgtaca ggagcaaaaa gaagattttt tttcgcggca tggctgtgta ttttttcaat
     3601 tttttcaaaa acggtcggag cgtttctgcc gccccgacct tcagaaaaat atcatccttt
     3661 cggagtcata ctactctttt gaggcggcgc ccgttgcgcg gtggctgccg atgcggtctt
     3721 cgggcttggc gccaaacagt tccgggtgca gatacttgcc tttatcgtcg gctttctttt
     3781 ccacttcagg aaccacccgg ttggccttcg cataggcgtc ctggcggatg cccgtcacct
     3841 gccagctcac ttccacgccg ggtttgtcgg tttggatgac gaagcgattg ccagccactt
     3901 tttccttcac aatggcttgc gcaaactgcc ctatgcaggt gagttggtag cggaattcca
     3961 tattgagcgc ctcgaaataa gccggcagtt ctaccgtagc cattccggcg gcatctgttg
     4021 tggcgttgcc gttgtagatg ttcatcatgt cggggctttc tacaaaactg tggtacaagt
     4081 acttatcagc cggagccagc gggtggtcaa tcttgaaagt gccggccgat ttggccagat
     4141 tgccgattac ttctacatcg ccgaaaaact cagccgcata gccgttgggg tcggtattca
     4201 taccgataat cgctgtttga ggcgctgttg tgacaatcgc ccttagttca cccgtgtgca
     4261 tggaacgcca gcggaagccg aaatcgccca gatcgtaagc attatctgcc gcgggaaaaa
     4321 tattgccgca agtgatgtcg ctgttcacag tcagttcgtc cacgatgcgc gctgtgcccc
     4381 atacgtcgag tttgtactgg ggcgaattgg tgccgatgcc cacattgccc tgctcagtta
     4441 tcaaagcatg gcccccaaaa ggggaggaga agaaaccgcc gtagccaaaa ttactgatgc
     4501 ccaaaacggc agcgccattt tcactgccgc ccgcaacacc cgcagcgcct ccataaccgg
     4561 caactcctgt tgatgtgccc gatccggtaa tgccgtaacc ggtattggga tgatccccca
     4621 ctacggtcaa ttggctgtgc ccgcttggcg tggtagtgcc gatggtgaca gtgcctccat
     4681 tggtgttata aatatttgac ccgttttccg tccatttgct gggcgctgca ttattgatga
     4741 taaaatctcc caaaaatccc ggcacgatac tgatgcccga accgcctagg tagttgctgc
     4801 ctccgccgga aggcaatgtc acggtagcgc cgtgtgtacc cgatccgttg gcgcccgata
     4861 tggagagtac gttgccgttg agcgagagcg tttgcaattc attgaccgga ctgttgtcgg
     4921 cggcgctgat gacggcattg ctgatgctga tgcccgagcc ggcagtgtag ttcccggcag
     4981 gcccctgcgg cccggtagcg cccattggcc cggcaggtcc ttgcggtcct attggtccgg
     5041 tagcgccttg cggccccgca atacccggca acccttgcgg ccctgcagca cccgttggcc
     5101 ccgtaggtcc ctgcggcccc attggcccgg tagcaccttg cggtccggcg ggtcccgtag
     5161 gcccttgcgg tccggcaggc aaagagacgg tattgccgcc cgaaatgctg agttgcgtac
     5221 cgttgacact gagggtttgt tcatcgcggt cggagccgga gctttctgca taaagcgcgt
     5281 agggtacgga gagcaattgg ctggtgccca tagggatgaa agcgccgccg tttgcgttgg
     5341 gattgagttc cacgcgcagg aaataggggc cggcgctcca gtcaacattg gccagactgc
     5401 cgccgccaat gggcgttccg ctgcctatat tgagatcaaa aacgcccagc gaagtagtcg
     5461 tcacggtatg ctcctcggca tatacggcgg ggccgttggc attgctttgg gctataaaaa
     5521 acctcagacg caaaggcgtg gaggcataag gctgcccgga ggcgtcgcgg gcaacggcct
     5581 gatacttgaa ttgttgaggc gattgagcag agatgtttgc cagaaggcag agcaggaaac
     5641 tgaaaacgag cattatcttt ttcatggttg ttgtgttttt tttggttttt aaaaaattga
     5701 gaatcagtga gtaatcacaa ttttaaaggt tttgaaacgg cgcgtttcag ggtcgcgggc
     5761 ctccagcagg tacaggccgg cagggagggc ggcggttttc aattcgtggt gatgatccgg
     5821 ctgggcggag gcaaatacct caatgccatg caaatgaagc agcctgatct gtacgggcgt
     5881 ttctgaatgg atgtagagcc ggtcggtact cggattggga aaggcccgta cttctgtcag
     5941 caactcggca ggcgactcaa cggcggtact caccgtgacg aacacctggt gaaaaccctg
     6001 ggtgagcatg gcggcatttt ggctgtaggt ggccgttgcg ggttctccta ccgaccagta
     6061 cagactgcct gccgaactgt tttctgcctg agcgccggca ctgcccagca ataccggtcc
     6121 aaggctctgg cccgatgctg ctgccgaaaa ggcaaggcac agccaggcca cgcataagcg
     6181 tagtgatttc ataatcaatg gattttaaaa gaagtgaaaa gaagaggagt atgcgggcag
     6241 cccggttttt acggcgccgc ccgccatttg ttcatggagg aattaatatc tttccaatat
     6301 cacattgccg gattctacga gtaccccgct ggtcgtgagg tgcaaagtag ctattgtgcc
     6361 tgctgtgttg gagcgcacca caatattgga gtgatatacg ggggtgttgt ctatgacgcc
     6421 aaacaaaccg gccgaattga caagggcaat gacatcatca gcgccttttt tgatccagat
     6481 atggcttccg cccgaaataa ccttgacgta ctcctgtgta ccgctgcctg accattcgat
     6541 gaacagattg ctggtttctt tggccgggcc gaggttgttg ggcgaggttc tttgcacaat
     6601 gatgtcttcc ataataatac ctacgggggt atcaatgatg gtagagagcg aggtgctgag
     6661 gccgcagcga cagcgcgaga atacctcaat ccggtagcgg gaattggggc tgaggccggt
     6721 tttgacgtaa ggcggcccgg ccactatatc ttcactcact accgtgccgg cgggcagcag
     6781 cgtggtgcgc actgtgtacc cgttgacggc agacacatcg gcgggcggcg tccaggtaat
     6841 ggcataactg acggagctga ggtaaagcac ctgcgagttg acaggcgcag ggcagtggca
     6901 gggtagcgcg gttttttgcg caacaactga ggcgatcatg gtaaaaaata gggccagaat
     6961 gatgccggca cgaaacgcga aattgagtgt ggttttcata atagtgttgt ttttagtatg
     7021 aactcgttga tgaatgatga cacaaagatg caaggcgggg gtggctatta ccaaagacaa
     7081 aaaagtgggt ttgttccatc ggggtaaata actgtaggaa aatgagcata tttgtcccgt
     7141 acttattcca cgaaacgcta tgaaaggcag tacgctattg ctaacgctca ggcttctgac
     7201 ggaagaggaa agggcagctt tagaactgat ggccgcatcg ccgctgttca aattgggcaa
     7261 ccggcatcag gatgcagtag agcttttgca atatctgcgc ccttttgccc cgcaatacga
     7321 gtctctggaa atggatcgcc aagtggcagc ccaggcgctt ttcgggcatc gcacacagcc
     7381 cgaagccgag ctgcgcaaag ccatgtccaa cctgaatact ttggtgcgaa agctcatttt
     7441 gttgcaacaa acccttacag acgacgacgg ccccgatgct caactgacgg ccgttcgcac
     7501 cgatctggca ctgatgcgct tttgcaacga acgcctcgac ttgagcgccg ccaaaaaaaa
     7561 atcgagcgcc aatgtatctg gtcgcaaagc caaacaaacc gatgatctgg tagctcaaac
     7621 ctacaacagc atcacagcaa aaacgcaatg cctccaaacc gaagcggccc aaaccttcac
     7681 cgcccggcaa tatttggaaa tgcgaaacat ccgcttagag gcggcacacg agtggcatgt
     7741 gcatcatctg ttcaacggtt caacagatgt caggcagttg ctggatatag tgttggagtt
     7801 ggagcaattg tatcattatc aacgccttga ccggcaagta tcgctgcgcg tgagtttgct
     7861 tggcatgaac acgcttcagg aggaagactc tgtgcgggcc gctctccatt atctggaggt
     7921 cgtgggcagt cggaagcccg ttttgcccgt tttatccgat acgcccatcg aactggaact
     7981 gtaccgacag gcgctcatca tgctccaaag ttttgacgcg ccggaaagca cggctgcttt
     8041 tgaatattta gataatcaat taaaaaacaa gcaattgccg ttaccggcag aacagataaa
     8101 aggactcaaa gcggtgctgc gattttattg cggcatcatg gccaaccgcc gacaggatgg
     8161 gtatttttta caaaagcggt tcgagttgtt tcaggagcat attgcagagg aaatgctgct
     8221 caatcggggt ggcgtctcgg cggttcagtt gtgcggattg ctgagcgatg ccctgcgact
     8281 gggcgatcac aaccatgcct gggcagagga ctttttgcaa cagtttgaaa acggggccca
     8341 aattgcccac acagaaacac cgcgggaggt ttataaaatc aacaaagcca atcttttatt
     8401 ccaccagggg cgcttccggg aaaccgccaa cgaactcatc ggctacgaat ggtatggccg
     8461 cgtggacaac ccccaaatcc tgctgctggc cgtgcgcata gacctcaaag cccgctatga
     8521 actcggcctg tacgacgacg atcacacact ccgcaccata gacgccgccg aaaaacgcat
     8581 ccttcgcctc gatgatttag acccccaact ggcgcgcatg acgctgttct ttttgcgtac
     8641 cgttcgccaa ttggcaggca tccggcacag aatgtcgcaa cccctcgcgt cgggcagccg
     8701 ccgttttgat gctgccggca aactcgaacg cctcgccggc gcaatagagg agcaaccgat
     8761 tgccgaaaaa gtatggctga aagagcaatt ggccgccgca ggaagcaccg gatgaagcga
     8821 actctgcgtt taaaacgcca gctccacgcc cgccgcaaaa ctccgtcccg gcatgcggta
     8881 gcgctccaat tcttcgtact gggtattgct gaagttgttg acagagaagt aaatacaatg
     8941 gcgctccttg atctggcggc tcaggcgggc attgccgatg agcgcagcgg cgggttcgct
     9001 tccggggtag atgaaaacct cctcgacgcg gctgcggtag cgcccgtcca aatgcagcat
     9061 ccagcccttg tggcgcagcg tggccgagcc gcccagagta tgccgcactt tgtaggcgag
     9121 ggcgtcgttg aagcgttcgg gcgagatgtc gcgggcatcc aaaaaagtat aggaaagact
     9181 cgtgctgagt gcctcgctcc attggcggcg ccacgaaatt tcgaggccct gcatcaacga
     9241 ttctttcagg ttgacgacct gataggtgag gggttcgagc ggctgtgaaa cctgctggaa
     9301 cgagatgaga tttttgtagc ggttgtaaaa cagcgccatg tccaaagcgc cggccttttt
     9361 tatgtctatg cgcgtaccca attcggcgga aagcaccaag cgttcgggcc gcagatcggg
     9421 attgggccgg aatgccagtc cgccgccctg ctcgaacttg acgaagcgct cggctaccgg
     9481 cggatcgcgg aaagcctggg ccagcaacat gcgccatgac cagtgcggtt tcatgcggta
     9541 gagtagggcg agttt
//
LOCUS       DAORLF010000147         9526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3866_length_9526_cov_2.195650, whole genome shotgun sequence.
ACCESSION   DAORLF010000147 DAORLF010000000
VERSION     DAORLF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_9526_cov_2.195650"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK198_12710"
     CDS             complement(<1..484)
                     /locus_tag="PK198_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39642.1"
                     /translation="MADEIKKLKFKENTDLQIEVVSLQNLTKSKKELLVIPHRTNFYH
                     IFLLENCQPTHLVDFEPIKIEPYSFLFINKDRVHQFDQLLNYEGKLIVFTEDFFCTTE
                     TDIKFLRSSILFNDLADQPTIKLDKSDFEKYISICENITEELLLPTDNAQHTLLKNLL
                     H"
     assembly_gap    1106..1192
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1485..3839
                     /locus_tag="PK198_12715"
     CDS             1485..3839
                     /locus_tag="PK198_12715"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF39643.1"
                     /translation="MTPRFFLTVFIAGVLFSGAFAQSLPSSMRISNDGRMLITGDQAS
                     TGLYDSATIRTIYLRFPQANYWTLMTQNYASQTDIPAEMEVDGVTYDSVGVRFKGQTS
                     YMGASASQKKSFNLSTHYIHEDQKIMGYKTLNLNNAFQDPSFLREVYYQNRIRRHVPA
                     AKSNFVRLNINGQDWGLYPNVQQLNKDYLEEWFLSNDGINWRADRPAGTGGGPGGPGG
                     GWGDGTAALNYLGADTTLYKQYYTLKSSDVDNPWSYLVAACQALNQTTIANLPSVLPN
                     YLDIDRTLWFLASEIAYSDDDSYVYKGKMDYYVYYEPETGRMTPLEYDGNSVMTTNAV
                     SWSPFYNANKVNYPLLNRMMQVPEWRQRYLAHLRTIITEQFEPAKANAIIDNYRAQID
                     ALVQADPKKLYTYNQFISELTVLKNFVNNRRNALLANTEVAQPAPTIHNAAYRNISGQ
                     EWTAPASGEQANVTAQVSFTTGLFQVRLYHAAGLTGNFTQVIMFDDGQHNDGAAGDGV
                     YGAAIPAYPAGSWVRFYIEAVANNPAKSVSYLPVGAEHDLFIYKVRSLALNGPVVINE
                     VMASNVSTVTDPAGQYDDWFELYNNSNEAVNLSGYFLSDKSDNPTKYRIPDGVSIEAN
                     GYLIFWADENGMQGPTHCNFKLSGDGEIVSLYRADTVLMDSVHFGAQIADVSYARVPN
                     GTGSFIQQAPTFNNNSTATEEPEAALPQMLIFPNPTADMAYIRLKNTAPNLQLPLMVF
                     DLRGQLLHQEIPAGSELELSTQNWPAGVYVVRYGTMAQRLVVRK"
     gene            complement(3927..>5479)
                     /locus_tag="PK198_12720"
     CDS             complement(3927..>5479)
                     /locus_tag="PK198_12720"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRF39644.1"
                     /translation="DPREMKPLLNGLFEGCMRGRTMYVVPYCMGPLGSPLSHIGVELS
                     DSEYVVVNMKIMTRMGKAVIDQLGEDGDFVECLHSVGAPLQPGQEDATWPCNPTVKYI
                     VHFPEERSIVSYGSGYGGNALLGKKCFALRIASAMAAEEDWLAEHMLILGVESPEGEK
                     TYVAAAFPSACGKTNFAMLIPPQAMDGWKVTTVGDDIAWIKPGSDGKLYAINPEAGYF
                     GVAPGTNYKSNPNCMETIKSNCIFTNVGVTADGDVWWEGMSKEVPAGVINWQGQVHDP
                     ASGKPVAHPNARFTAPASQCPSLDSDWENPNGVPVSAFIFGGRRSTTVPLVYQSFNWN
                     FGVYMAATMGSEMTAAAFGELGKVRRDPFAMLPFIGYNMAEYFNHWLEFGRNLPNAPR
                     IFGVNWFRKDEEGNFLWPGFGENMRVLKWVVDRVRGRAAAVECPIGWMPRYRDMEWSG
                     LKFDKEDFKKLMTIDREAWKKELLAHEELFSQLYDKLPKEFFFMRELLLSSLWRSPEY
                     WGQAGERE"
     assembly_gap    5480..5502
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5503..5801)
                     /locus_tag="PK198_12725"
     CDS             complement(<5503..5801)
                     /locus_tag="PK198_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39645.1"
                     /translation="MTKTMSSVTLPKDYSDTVRHERLIQWVQKMAAHCQPDQIYWCDG
                     SEEEYKLFCNMLVDKGTFIRLNEEKRPNSFACFSDPSDVARVEDRTFICSRLREDA"
     assembly_gap    5850..5862
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6027..>6863)
                     /locus_tag="PK198_12730"
     CDS             complement(6027..>6863)
                     /locus_tag="PK198_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRF39646.1"
                     /translation="KEARKIVEKVVLAATAREAARKAREMVQRKNVLTGSGLPGKLAD
                     CSSKDPAESELFLVEGDSAGGTAKQGRDRVFQAILPLRGKILNVEKALEHKIYENEEI
                     KNIFTALGVSITEDENGERRLNLEKLRYHKIVIMCDADVDGSHITTLILTFFFRYMHD
                     LVKQGYVYIASPPLYQVKGTKGQFRYCWNEIERKQAQEELARGGNPDSVKVQRYKGLG
                     EMNAEQLWETTMDPTRRFLRLVNIEDAREADRVFSMLMGDEVPPRRAFIEANAKYAKV
                     DA"
     assembly_gap    6864..6873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6874..8015)
                     /locus_tag="PK198_12735"
     CDS             complement(<6874..8015)
                     /locus_tag="PK198_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39647.1"
                     /translation="METTNENKDLQAKAGDYDASNIQALEGLEAVRKRPGMYIGSTDS
                     KGLHHLVWEVIDNSIDEHLAGYCDLIETTIHTDNSITVRDNGRGIPTGMHEKLQKSAL
                     EVVLTVLHAGGKFDKNTYKVSGGLHGVGVSCVNALSDYLRAEVHREGKVFEQEYSKGI
                     PQTTVSVIGESGRTGTTITFRPDATIFEATEYKYDILAARMREMAFLNKGIRMTLTDE
                     RETGPDGQYKTEEFHSEGGLVEFLEYIDNSKHRITESPIQVSASADDVVVDVAFQYNS
                     SYSENVYSFVNNINTREGGTHVNGFRMAVTRLLRQYGEEKDLFKKIKVKPSAEDFREG
                     LSAIVSVKVPEPQFKGQTKGELGNAEVTGIVSRIVGEAMGHFLEENP"
     gene            8129..9064
                     /locus_tag="PK198_12740"
     CDS             8129..9064
                     /locus_tag="PK198_12740"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRF39648.1"
                     /translation="MKFPHPVPVRELAARLNAEIIGDADALATGLNEVHHVAPGDITF
                     VDVAKYFEKALRSAATVVLLNERTAAPSGKTLLLCEKPFDAYNQLALEYRPLLPLTAN
                     ISPTAEIGEGTHIEPNAVIGPHVHIGRNCHIGANAVIAEHTIIGDRVLIQPGALIGTE
                     AFYYKKTAEGFIKWRSCGRVVIEDDVEIGAGCTVNKGVSSDTTIGEGTKLDSQVHVGH
                     DVRIGKRCLIAAQVGIGGNTTIGNEVILYGQAGVAQNICIGDKAIISAKAGVSKDLDG
                     GKLYFGIPATEARIAYRELAALRLLPEFFAEYYKS"
     gene            complement(9069..>9526)
                     /locus_tag="PK198_12745"
     CDS             complement(9069..>9526)
                     /locus_tag="PK198_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39649.1"
                     /translation="FSSFFADDPGEHYYGLRARAGMKYFFSLRPEASWYAGFEAKYND
                     IQHAYWERLLRQGGQFEQVFLVDRSVKSTGAALRIGAQFYFNADRRLLLDVYTGIGFM
                     ANHVAESNRPPDAEPITRGLFSADDFMYLNGRHTIADYLLGMHIGYSFW"
BASE COUNT         2125 a         2558 c         2423 g         2287 t
ORIGIN      
        1 tatgaagtag attttttaat aaagtatgtt gtgcattgtc agtaggcaaa agcaattctt
       61 ctgtgatgtt ttcgcaaatg ctgatgtact tttcaaaatc gcttttatcc aattttatgg
      121 tcggttggtc tgcaaggtca ttaaataaaa tactacttct taaaaattta atgtcggttt
      181 ccgtagtgca aaaaaaatct tctgtaaaca cgatgagttt tccttcataa ttcaataact
      241 ggtcaaactg atgcactcgg tctttattaa tgaaaaggaa agaataaggt tcaattttta
      301 tcggttcaaa atctactaaa tgtgttggtt ggcaattttc aagcagaaaa atatggtaga
      361 aattcgtgcg gtgcggaatt actaaaagtt cttttttcga cttggttaaa ttctgcaacg
      421 atactacttc aatttgaagg tcagtatttt ccttgaattt cagttttttt atttcgtccg
      481 ccattgatta tttgaatgca caaagataag tttttgtttg taggttgctg tttgaacggt
      541 cgctggtagc attacgccta actcccgctt gtcgaaatca tccctattgc gcctattgcg
      601 ccaacgcacc cgacgctggt tgtattgacg ccataaggac catacaaaac ccgcattgcg
      661 tgttttactt agtcgtcgtg gcgttaatat ccgctatgta cgcatccacc cctccgccta
      721 tccggcgcac ttctgcccgg ttaccttcat tccaacctca atagaaactt cctgcgttca
      781 gttttttcac aggagtccat tctggtttcg actgtgcaaa gctaatgtaa gtttgaaata
      841 aaatcaactg tgccataaaa aaactttttc acaggttgag tctatccaaa gaacttcacc
      901 cccgctgccg gcctacgcgt aatgccgcgc acacctccct gtcttgctgt tttcgtggcc
      961 tgtgcgggta cagcatattc gtctccccgg cgtcataaaa acaatacccc gggtacatct
     1021 tttaatgtta agcaattgtt aaagcctgtt gctgcggcaa cttgggaagc tgttttgtac
     1081 ccaagcattt ggagttccgg caggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtgtttc
     1201 gcgttgattc gcgttcagag ttccggggtt ccctctcgtt cgcggaattt cgcgtccttc
     1261 gcgttgattc gcgttcagag tttcgggttt caggttcaag gttccgggtt taaagtttcg
     1321 cgttcaaagt ttcgcgttcg aggttcccca cgcttagcac cgccgaccgc ataccgcaca
     1381 ctgtttccgg gttcaagatt ccggcgttct ctcgcgctta gcaccccgcg ccatggcgcg
     1441 acacaccgac acaattatca tcatactttc ataaaatcat gccgatgaca ccacgttttt
     1501 tcctcaccgt ttttattgcc ggcgtcctgt tttcaggcgc tttcgcgcaa tccctcccct
     1561 cctccatgcg catttccaac gacgggcgca tgctcatcac cggagaccag gcctccacag
     1621 ggctgtacga ttctgctacc atcagaacca tttatctccg gtttccgcaa gccaattact
     1681 ggaccctgat gactcaaaat tacgcctccc aaaccgatat tccggcggaa atggaagtgg
     1741 acggcgtcac ttacgacagc gttggcgtgc ggttcaaagg gcaaacttct tacatggggg
     1801 cctccgcttc tcagaaaaaa tcgttcaacc tctccacgca ttacatacat gaggatcaga
     1861 agattatggg ctacaaaacc ctcaacctga ataatgcctt ccaggacccc tccttcctgc
     1921 gcgaggtgta ttaccaaaac cgaattcgac gccatgtacc ggctgccaag tccaattttg
     1981 taaggctcaa tatcaatggc caggattggg gcctgtaccc caatgtgcag caactcaaca
     2041 aagactatct cgaagaatgg ttcctcagca acgacggcat caactggcgg gccgaccggc
     2101 ctgcgggaac cggcggtggc cctggcggac ctggcggcgg ttggggcgac ggcacggcgg
     2161 cactcaacta cctcggcgca gataccaccc tctacaaaca gtattatacc ctgaaaagca
     2221 gcgatgtgga caatccctgg agctatctgg tggccgcctg tcaggcactc aaccagacta
     2281 ccatcgccaa cctgccctcc gtgctgccca attatctcga cattgaccgc acgctgtggt
     2341 tcttagcttc cgaaatcgcc tattccgacg acgacagcta tgtgtataaa ggcaaaatgg
     2401 attactacgt ttattacgag ccggaaaccg gccgcatgac gcccctcgaa tacgacggca
     2461 acagcgtgat gacaaccaat gccgtgagct ggagtccgtt ttataatgcc aataaagtga
     2521 actaccccct acttaacaga atgatgcagg tgcccgaatg gcggcagcgc tatctggctc
     2581 atttgcgcac catcatcacc gagcagttcg aaccggccaa agccaatgcc atcattgaca
     2641 actaccgcgc tcagattgac gcgctggtgc aggccgatcc caaaaaactt tatacttaca
     2701 atcagttcat cagcgaactg accgtgctga aaaactttgt caacaaccgg cgcaatgcct
     2761 tgctggccaa taccgaagta gcacagcctg cgcctaccat tcacaatgcg gcttatcgca
     2821 acatttccgg gcaggagtgg acggcgcccg cttccggcga gcaggccaac gtgaccgctc
     2881 aggtgagctt taccaccggc ttatttcagg tgcgcctcta ccacgctgcc ggcctcacgg
     2941 gcaatttcac gcaggtcatc atgttcgacg acggccagca caacgacggc gctgcgggcg
     3001 acggcgttta cggcgctgcc atccccgcct accctgccgg ttcctgggtg cggttttaca
     3061 tcgaggcggt ggccaataac cctgccaaat cggtgagtta cctgcctgta ggcgccgagc
     3121 atgacctgtt tatttacaaa gttcgttccc tcgccctcaa tggcccggtg gtcatcaatg
     3181 aagtcatggc ctccaacgtt tctaccgtta ccgaccctgc cggccaatac gacgactggt
     3241 ttgaactgta caacaactcc aacgaggcgg tgaacctcag cggctatttc ctttccgata
     3301 aatccgacaa cccgaccaaa taccgcatcc ccgacggcgt gagcatagag gccaacggct
     3361 atctcatctt ctgggccgat gaaaacggca tgcaagggcc tacgcattgc aattttaagt
     3421 tgtcgggcga cggggaaatc gtctcgctgt acagggcaga caccgtgctg atggattcgg
     3481 tgcatttcgg cgctcagatt gccgacgtga gttacgccag agtacccaac ggcacgggca
     3541 gtttcattca gcaggcgccc acgttcaaca acaacagtac agctacggaa gaaccagaag
     3601 ccgcgttgcc gcaaatgctg atttttccca acccgactgc cgacatggcc tatatacgat
     3661 tgaaaaatac cgcacccaat ctccaactgc ctttgatggt gtttgacttg cgggggcagt
     3721 tgctgcatca ggaaataccc gccggcagcg aactggaatt gtccacccaa aactggcctg
     3781 ccggggtata tgtggtgagg tacggtacga tggctcagcg tttggttgtg aggaagtaat
     3841 ttcttgtatt cctcctaaaa aatgagcggc gcagtaccca aaagaggtgc tgcgccgcat
     3901 gtatttatca acgtaagtat aaacatttac tccctttctc ccgcctgtcc ccagtattcg
     3961 ggcgaacgcc acaaggaaga cagcaggagt tcgcgcatga agaagaactc cttgggcagt
     4021 ttgtcgtaca actgcgagaa aagctcttcg tgtgccaaca actctttttt ccatgcctcg
     4081 cggtctatcg tcatcagttt tttgaaatct tctttatcga atttcagacc gctccattcc
     4141 atgtctctat aacgcggcat ccaaccgatg gggcactcca cagcggcagc ccgaccgcgc
     4201 acgcgatcca ccacccactt gagtacgcgc atgttttcgc cgaagcccgg ccacaggaag
     4261 ttgccctctt cgtctttgcg gaaccagttg accccgaaaa tgcggggagc attgggcagg
     4321 ttgcgaccga attccagcca gtggttgaaa tattcggcca tgttgtagcc gatgaaaggc
     4381 agcatggcga aggggtcgcg acgcactttg cccaactcgc cgaaagcggc tgccgtcatt
     4441 tcagaaccca ttgtagcagc catatacacc ccgaaattcc agttgaacga ctgatacacc
     4501 aaaggcaccg tcgtactgcg gcgaccaccg aaaatgaacg cgcttacggg cacgccgttg
     4561 gggttttccc aatcggaatc aagggaaggg cactgcgagg ccggcgctgt aaagcgggcg
     4621 ttgggatgcg ccaccggttt gcccgatgcc ggatcgtgta cctggccctg ccagttgatg
     4681 acgccggcgg gaacctcctt gctcatgcct tcccaccaca catcgccgtc ggctgtgacg
     4741 cctacattgg taaaaatgca gttggatttg atggtttcca tgcagttggg gttgctcttg
     4801 tagttggtac ccggcgccac gccaaaatag cccgcttcgg ggttgatggc gtagagcttg
     4861 ccgtcggagc cgggtttgat ccaggcaatg tcgtcgccca cggtggtcac tttccagccg
     4921 tccatggcct gaggcggaat gagcatggcg aagttggttt tgccgcaggc gctggggaaa
     4981 gccgccgcca cataagtctt ttcaccttcc ggcgattcta cgccgaggat gagcatgtgt
     5041 tcggccagcc agtcttcttc agcagccatg gcgctggcaa tgcgcaatgc gaagcacttt
     5101 ttgcccaaaa gggcattgcc gccgtagccg ctgccataag aaacaataga gcgctcttcg
     5161 gggaagtgta cgatgtattt tacggtagga ttgcagggcc atgtggcgtc ttcctgaccg
     5221 ggctggagcg gggcgcccac cgagtggaga cattctacaa aatcaccgtc ttcgcccaat
     5281 tggtcaataa cggctttgcc catgcgggtc atgattttca tattgaccac cacatactcg
     5341 gaatcgctca gctccacgcc gatatgggag agcggcgaac cgagcggccc catgcagtaa
     5401 ggcaccacat acatggtacg gccgcgcata cagccctcga acaggccgtt gagcagcggt
     5461 ttcatttcgc gggggtccan nnnnnnnnnn nnnnnnnnnn nngcgtcttc gcgcaagcgg
     5521 ctacagataa aggtgcggtc ttctacgcgg gccacatcgc tgggatcgga aaagcaggcg
     5581 aagctgttgg ggcgcttttc ttcgttgagg cgaatgaaag tgcctttgtc caccagcata
     5641 ttgcaaaaca acttatattc ttcctcggag ccgtcgcacc agtagatttg gtctggttgg
     5701 cagtgtgcgg ccattttctg aacccattgg atgagccgtt cgtggcgaac ggtatccgaa
     5761 tagtcttttg ggagtgtaac ggatgacatg gttttggtca ttggttatga tgattatggg
     5821 tgaaaaaatc aaaaaaacaa gccccttctn nnnnnnnnnn nncttctgca atgcgaacat
     5881 tgcttcaaag tacatcaaca aaaggtagga aagtgccctg agaagcggct tttacgctgc
     5941 aaaattatgt agcccgatgc acaaagggcg ctgattttta tcacctgcct cggtgatctt
     6001 tccaatatcc cattacacct gcaacatcag gcatccacct tggcgtattt ggcgttggcc
     6061 tcaataaagg cgcggcgcgg cggtacttca tctcccatga gcatggagaa tacgcggtct
     6121 gcttcgcggg cgtcttcaat attgaccaaa cggaggaagc ggcgggtggg gtccatggtg
     6181 gtttcccaaa gctgctcggc attcatctcg cccaaaccct tgtatcgctg cactttaacg
     6241 gaatcggggt tgccgccgcg agccagttcc tcctgagcct gtttgcgttc aatttcgttc
     6301 cagcagtagc ggaattgccc tttggttcct tttacctgat acagtggcgg cgaggcgatg
     6361 taaacgtagc cctgcttcac gaggtcgtgc atgtagcgga agaagaaggt gagaatgaga
     6421 gtggtaatgt ggctgccgtc cacgtcggcg tcgcacatga tgacgatttt gtggtagcgc
     6481 agtttttcca gattaaggcg gcgctctccg ttttcgtctt cggtgatgct gacgcccaat
     6541 gcggtgaaga tatttttgat ttcttcgttt tcgtaaattt tgtgttccag cgctttttcc
     6601 acgttgagga ttttgccccg cagcggcaga atggcctgaa acacgcggtc gcggccctgc
     6661 ttggcggtac cgcctgccga atcgccctcc actaagaaga gttcagattc ggcggggtct
     6721 ttggaggaac agtcggccaa tttgccgggc aatccgctgc cagtgagcac gtttttgcgc
     6781 tgcaccattt cgcgggcctt gcgggcggct tcacgggcgg tggcggccag cactactttt
     6841 tctacgatct tgcgggcctc tttnnnnnnn nnnggatttt cttccaaaaa atgccccatg
     6901 gcctcgccta caatgcgcga cacgatgccc gtcacttcgg cattgcccaa ttcgcctttg
     6961 gtttgtcctt tgaactgagg ctcaggcact tttacggaca cgatggccga caatccttcg
     7021 cggaagtctt cggcggaggg ctttactttg atctttttga agaggtcctt ttcttcgccg
     7081 tattgacgca gcaaacgcgt gaccgccatg cggaagccgt tgacgtgcgt gccgccctcg
     7141 cgggtgttga tgttgttgac gaaggaatat acattctccg agtaggagct gttgtactgg
     7201 aaggccacat ccaccaccac gtcatcggca gaggcagaca cctgaatcgg gctttctgtg
     7261 atgcggtgtt tggagttgtc tatgtattcc aaaaactcca ccaaaccgcc ctccgaatga
     7321 aattcctcgg ttttgtattg gccgtcgggg ccggtttcgc gctcgtcggt cagggtcatg
     7381 cggatgcctt tattgaggaa ggccatttcg cgcatgcgag cggcgaggat atcgtatttg
     7441 tattccgttg cttcaaaaat ggtggcatcg ggacggaaag taatggtggt tcccgtgcgg
     7501 cccgattctc caatgacgct caccgttgtt tgcggaatac ctttggagta ctcctgctca
     7561 aagaccttac cttcgcggtg tacctcggcg cgcaggtagt cggacagcgc gttgacgcaa
     7621 gacacgccca cgccgtgcag accgccggat actttgtagg tatttttgtc aaacttgccc
     7681 ccggcgtgca gcaccgtgag taccacctcc agcgccgact tttgcagttt ctcgtgcata
     7741 ccggtgggaa taccccggcc attgtcccgc accgtgatgg agttgtcggt gtgtatggtg
     7801 gtttcgatga ggtcgcaata acctgccagg tgctcgtcta tggagttgtc aataacttcc
     7861 cataccaagt ggtgcagacc tttggagtcg gtgctgccga tgtacatccc tggtcttttg
     7921 cgcacggctt ccagaccttc gagggcctga atattactgg catcatagtc gcctgcttta
     7981 gcttgcaggt ctttgttttc gttcgttgtt tccatgccgc aaaggtacaa aaaagacctc
     8041 tactttgaag ttttgctttg attgatttta cagctataaa aagagcgatt cagcgccgat
     8101 acgagtacct ttgccgcaaa aaaaggacat gaagtttcca caccccgtgc cggtaagaga
     8161 gctggcagcc cgattgaacg cagaaatcat cggcgatgca gatgccttgg ctaccgggct
     8221 gaacgaagta caccatgtag cgcccggcga catcactttt gtggacgtgg ccaaatattt
     8281 tgaaaaggca ttgcgctcgg cggctaccgt tgttttgctc aacgaacgca ccgccgcgcc
     8341 ttccggcaaa acgctgctgc tgtgcgaaaa gccttttgat gcgtacaacc aattggcgct
     8401 ggagtaccgc cccttgctcc ccctgacggc caacatcagc cctacagccg aaatcggcga
     8461 gggtacgcac atcgagccga acgccgtcat aggaccgcat gtacacatag ggcgtaactg
     8521 ccacatcggg gccaatgccg tcattgccga gcataccatc atcggagacc gggtactcat
     8581 tcagccgggc gcgctcatcg gcacggaggc tttttattac aaaaaaactg ccgaaggttt
     8641 catcaaatgg cgctcttgcg gccgcgtggt cattgaagac gatgtggaaa ttggcgcggg
     8701 ttgcaccgtc aataaaggcg tatcaagcga tacgaccatc ggagagggca ccaaattgga
     8761 cagtcaggta cacgtgggcc acgatgtgcg catcggcaag cgttgcctca ttgctgcgca
     8821 ggtaggcatt ggaggcaata caacgattgg caatgaggtc attttgtacg gccaggcggg
     8881 ggtagcccag aacatctgca tcggagacaa ggccattatt tcggcgaaag ccggggtgtc
     8941 gaaagatttg gacggcggca aactgtactt cggtattcct gccacagagg cgcgtatagc
     9001 ttaccgcgag ttggcggcgc ttcgcctgct accggagttt tttgcggagt attataaaag
     9061 ttagaaacct accaaaacga atacccgatg tgcatgccca gcaagtagtc ggctatggta
     9121 tgccgcccgt tcagatacat gaaatcatcg gcagaaaaca gaccgcgcgt tataggctcc
     9181 gcatccggcg ggcggttgct ttcagccaca tgattggcca taaaaccaat gccggtgtaa
     9241 acatccagca ggaggcgcct gtctgcattg aaataaaatt gcgctccgat tcgcaaagcg
     9301 gccccagtgc tctttacact tctgtcaacg aggaaaacct gctcaaactg gccgccctgc
     9361 ctcagcagcc gttcccagta ggcgtgttgt atgtcgttgt atttggcttc gaaacctgca
     9421 taccaggagg cttcgggccg cagcgaaaaa aagtacttca tacccgcccg cgcccggagg
     9481 ccgtagtagt gctcgcccgg atcgtcggca aaaaaagagg aaaaaa
//
LOCUS       DAORLF010000148         9450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3908_length_9450_cov_2.310591, whole genome shotgun sequence.
ACCESSION   DAORLF010000148 DAORLF010000000
VERSION     DAORLF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_9450_cov_2.310591"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PK198_12750"
     CDS             complement(<1..1237)
                     /locus_tag="PK198_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF39650.1"
                     /translation="MKDLFCRYQTLKGKRVERKGGWDTHGLPIELNVEKELGITKEDI
                     GKTITVDEYNQKCRETVMRFKDEWDDITRKMGYWVDLEHPYITFENNYIESVWWLLQR
                     LYDKGLLYKGYTIQPYSPAAGTGLSSHELNLPGCYKEVSDTTVVAMFKVVKDEKSAFL
                     YDSEDEDVRILAWTTTPWTLPSNTALTIGKNIEYVKIKTANPYTQQPVSVVLAQDLTG
                     KWFGSKNQPEVQASSAPIFSDQLEDVRYEPLLDFGNPIEPLLGTNGYGTEAYRVLIGD
                     FVTTEDGTGVVHTAPSFGADDMRVAKSNGIGALTLVDKQGKFKDTVGEFSGRYVKNYT
                     NDPAWENVDVDIAVHLKQSGKAFNVQKYVHNYPHCWRTDKPVLYYPLDSWFIRASAAR
                     ERMYELNQTINWKPAATGDG"
     gene            2254..2904
                     /locus_tag="PK198_12755"
     CDS             2254..2904
                     /locus_tag="PK198_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRF39651.1"
                     /translation="MSNMVVRYSDADLAEFKEIIDKKLEDAQANLRSLQEQILEITEN
                     TSDEHGGDWMDDSNINNEMEMLNNMAIRQRMYIKDLENAQVRIRNKSYGICSISGELI
                     DKKRLLAVPTTTKSLSAKAEEAKNKDDKPMPKPLVKPIPAPDNSPKVITKVIRKSAGP
                     TGKPNPTLDDDDDFIFDREVFMDEEPDEEEEEDDSMEQNVSDMDDYADTRDDDSDY"
     gene            2950..>3268
                     /locus_tag="PK198_12760"
     CDS             2950..>3268
                     /locus_tag="PK198_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39652.1"
                     /translation="MHRRTFLRSTAFLGAAVVVNPVQFCTPSSSKLPGQKAVGLQVYT
                     LRDLMAKDPEGTLKAVADLGYNYIELFNYREGMYFGKTVKEMQEFLQGIQLPVFSSHI
                     PTGA"
     assembly_gap    3269..3290
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3291..>3661
                     /locus_tag="PK198_12765"
     CDS             <3291..>3661
                     /locus_tag="PK198_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRF39653.1"
                     /translation="TVLNDVERAVADAKMLGQEYLVCPWLADSERKTIDDYKRLSVAF
                     NKAGEVCKQYGIQFCYHNHDFEFFKLDGQIPMDVILAETDAKLVQIELDIYWITKAGF
                     SYADYFKRYPGRFPLWHVKDM"
     assembly_gap    3662..3743
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3854..>4784
                     /locus_tag="PK198_12770"
     CDS             3854..>4784
                     /locus_tag="PK198_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HRF39654.1"
                     /translation="MKTYYSFALALAAILMLVACQPTDNKSTATSPGSAETAASKPAN
                     APAYRHLKGQVGTLAVVMDLAYRPAGDDEFSTDMYHGSYYYSSKENPIDLYGSVDSTG
                     QLVLYEVTLNDDTSPTFTGSFDQEQGVFKGEWSSGDGKKKMPVELHENYDDAVRFTTY
                     ELEASQDLFEGKENSPYATISKIWLQPAAGTDATLASFLKKIIHQGLVGDSLATLYPI
                     QEKAFEELSKDYFASYRTDMGDVKEEELGPDDSFMFTYEQNASVEVLYNQNQLLTLGY
                     WDYWYTGGAHGNYGTWLASYDLAAQKEITLQDVF"
     assembly_gap    4785..4794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4795..5049
                     /locus_tag="PK198_12775"
     CDS             <4795..5049
                     /locus_tag="PK198_12775"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRF39655.1"
                     /translation="KITPALEKAVRSRFGISAKEPLSSVLFEDAIAATENFGLTGKGV
                     IFNYPPYEIAAYAAGEIRLFVPYSEIKNLLQPAFAAKYVK"
     gene            5190..5723
                     /locus_tag="PK198_12780"
     CDS             5190..5723
                     /locus_tag="PK198_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39656.1"
                     /translation="MPTWLSVILEIIKISVPALIVFFTVHTLLKQYLAGQERMKALEL
                     QQNRLGSTTPLRFQAYERLSLLCERIALPNLIMRMRPETANVAEYRLALMLAIQQEFE
                     HNITQQVYVSEQLWEIIKMARDHTVEVINISAEKLPPKSEAKELAQFILSLPPENTTG
                     ALDKALAAIKREAAAML"
     gene            complement(5811..7340)
                     /locus_tag="PK198_12785"
     CDS             complement(5811..7340)
                     /locus_tag="PK198_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39657.1"
                     /translation="MKLKAIFIPVLFIISTHLSAQKHDYNWLFIEEFYPNNGFGEAAR
                     MNFNTIPPTISAPDNVLITAWSSVAQMSDADGELIFFTNGCEIHDASFNLMENGHDIN
                     AGAVHDYVCYPPRITPGYTAGPQSCMAVPMPGSDRYYYLFHIANEYPPGSSYGVFCIL
                     HYSKIDMYGNGGLGAVIEKNVRIIDYSNDFTPIMNLTAVRHTNGQDWWIISPYLWGNG
                     YHKILLTDMGVVSLTEQYIGQDRTGAFFSQITFSPDGTLFAHCTNDLGVQLYDFDRST
                     GEFSNPRYFYIQPASGWLAGVGFSPSGRFVYASINLVLYQYDLWAPDVAASRVLVAEY
                     DGYADPIPTTFYMMQLGPDCKLYMFCSSCTTIHVIREPDLPGLACEVVQNDVQLPWGM
                     RGGGAPTFPNYRLGALGEPVAPCGPVSGTAPTLPVEAGLHLYPNPAAMAVTVEWEGAP
                     VAAGMITVRNSAGKEVLHTRAAFFSGSTELLVGMLPPGWYSLYVQVGAERRVARLVVQ
                     R"
     gene            complement(7845..>9450)
                     /locus_tag="PK198_12790"
     CDS             complement(7845..>9450)
                     /locus_tag="PK198_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc carboxypeptidase"
                     /protein_id="HRF39658.1"
                     /translation="EQGGIGAGRALIIENGDTLTLRDRVTHHRTAALSTVEVASQHVA
                     DLVKNFQAHYDRSRTNPPGRYKAFIIKASNPAGKLAAFAELLDRNGIRYGKAGKNASL
                     KGFDYIKGAAGTAFKIEENDLVISAYQPAGTMAQILMEPQTQLADSVTYDITAWSLPY
                     AHGLEAYALEERLEPQGAFALPAPAALPAGAAPYAYLVKWNSMNAARFLADVSQQGIG
                     VRFARSPFRIEGQDYAAGTLIITRADNRLAADFLNQTVSEAIKKHKVDAAIVRSGMVE
                     KGFDFGSDAVPMAARPRIAVLSGEGTSANSYGQVWYYLEKDLGYPFAALPANNLGSLD
                     LSNYDLLIMADGGYGLDEGAAGRLSQWISKGGRLIAVGYALNSLQGKSGFALTRYESK
                     DAENKAEREAEKAELDSRLLNFGDQERRAISGEIPGAIFKVKMDITHPLAFGMGEHYF
                     SLKTGAGAYPWLKGAWNVGSIGDNPNYYGFVGQYAQQQVKNSAVFAVEDKGRGAVIYM
                     VDNPLFRGFWENGKLLFSNAVFFTGQ"
BASE COUNT         2293 a         2409 c         2444 g         2190 t
ORIGIN      
        1 gcccgtcgcc ggtggcggca ggtttccagt tgatggtctg gttgagttcg tacatgcgtt
       61 cgcgggcggc ggaggcgcgt atgaaccaac tgtccagggg gtagtacagc acgggtttgt
      121 cggtgcgcca gcagtgcggg tagttgtgta catatttttg cacattgaag gccttgccgc
      181 tttgtttgag atgcaccgca atgtccacgt ccacattttc ccaggcgggg tcgttggtgt
      241 aattcttgac gtagcggccc gaaaactcgc ccaccgtgtc tttaaatttg ccctgcttgt
      301 ccaccaaagt gagtgcgccg atgccgttgg acttggccac gcgcatgtcg tcggcgccga
      361 aactgggagc cgtatgcacc acgccggtgc cgtcttcggt ggtgacgaaa tcgccaatca
      421 gcacgcgata agcctctgtg ccgtagccat ttgtgccgag cagcggctca atggggttgc
      481 cgaaatcgag gagcggctca tagcggacgt cttcaagttg atcggagaaa ataggcgcgg
      541 aggatgcctg tacttccggc tggttcttgc tgccgaacca tttgcccgtc aaatcctgcg
      601 ccagcactac gctgaccggc tgctgggtgt agggattggc ggttttgatc ttcacatatt
      661 cgatgttttt gccgatggtg agcgccgtat tggagggcag cgtccaaggg gtagtcgtcc
      721 aggcgaggat gcgtacatct tcgtcttcgg agtcgtacag aaaggccgat ttttcgtctt
      781 tcactacttt aaacatagcc acgacggtgg tatccgacac ctccttgtag cagcccggca
      841 ggttgagttc gtgcgaactg aggcccgtgc cggccgccgg cgagtagggc tgtatggtgt
      901 agcctttgta gagcagccct ttgtcataca ggcgttgcag cagccaccac acactttcta
      961 tataattatt ttcaaaagtg atgtacgggt gctccaggtc cacccagtag cccattttgc
     1021 gggtgatgtc gtcccattcg tctttaaaac gcatcacggt ttcgcggcac ttttggttgt
     1081 actcgtccac ggtgatggtc ttaccgatgt cttctttggt aatgcccagt tccttttcca
     1141 cgttgagttc gatgggcagg ccatgcgtat cccagccgcc tttgcgttct acgcgcttgc
     1201 ccttgagggt ctggtagcga cagaagaggt ctttgatggt gcgggcaatg acgtggtgaa
     1261 tgccgggctt tccgttggcc gatggcgggc cttcgggtga cgtggtcgtg gacggtcgtg
     1321 gcgagcgtcg gcgccgtcgc gctgttctat gctttgttta aaaacatcgt gttcttccca
     1381 gaatcggagg atgtctttat caatttcggg caggttgaga tttttgtacg tcgggtacat
     1441 ggtttgtgtg cttaaaaagt gaagtgccag gcattacggg ccgcaaagat agcgaaaaca
     1501 aaaaagctcc ggcaagtgca atctgccgga gctttttcga ttttcaaaac gcatcagctc
     1561 agcgattgca atgcaaggcc ggaataataa tgctggtgcg aatgatctgt tgcatactct
     1621 gtaaaatgct gaacgaccgc aaagatacgc agtcgttctg aaaaaaacaa gctgtgagag
     1681 aaggattcga acctccacga ggcggttagc aacagcgcaa atatttagag agagctactc
     1741 tcctccgaat ggtggtcaac cccagtcggt gacgcccggg cgtcatcggc gttacgctgt
     1801 gtttatccct gagtcctcac ccccgagaca agagggcatg gctgccaatt tcatcacctc
     1861 acaatgtagt ggccggtagc gcgttcatac tgctcgccgg ctgcctgtgc agcgctttcg
     1921 ttaccgacgg tgcaaagata aaggcattgt cttcttttgt gcaaattttt taagaattat
     1981 tttaaaaaaa tataaattat tcaatttttt gatgctttcg ttgttaatat cgcaattcat
     2041 gtatgacctt tggaggccaa aattcgcaca atgcacggat gcaggaaacg atgtcgcttc
     2101 cggaaagcag gatgcaagta taaaatgcgg cttggtaaag aaacataaaa ttaacatgca
     2161 accaagtgcc cgcatatttg tttttttcgg tttgtaaatt cgcatattcg tatatttgcg
     2221 cttcattttt caaaaccata aaaccagacg aagatgtcca acatggttgt aaggtactcg
     2281 gatgccgatt tggcagaatt taaagagatc attgacaaaa aattagagga cgcccaggcc
     2341 aatctcagaa gtttgcagga acagatactg gaaataactg aaaataccag cgacgaacat
     2401 ggcggcgact ggatggacga cagcaacatc aacaacgaaa tggaaatgct caacaacatg
     2461 gccattcgtc agcgcatgta catcaaagac ctcgaaaatg ctcaggtgcg cattcgcaac
     2521 aagtcgtacg ggatatgttc tatttccggc gagttgatag ataaaaaacg cctgctcgcg
     2581 gtacccacta ccaccaagag cctctcggca aaagcagaag aagccaaaaa caaagatgat
     2641 aagcccatgc ccaagccttt ggtaaaaccc attccggcgc cggacaattc gcccaaggtc
     2701 attaccaagg tcatccgcaa atcagcaggt ccaacaggca agcctaatcc tacgcttgac
     2761 gacgacgatg attttatctt tgatcgcgag gtgttcatgg atgaggaacc cgatgaagag
     2821 gaggaggaag acgattccat ggagcaaaat gtatcggaca tggatgatta tgccgatacc
     2881 cgcgacgacg attcggacta ttaggaagcg ccgaaaagtc attggtagcc atcacatttc
     2941 acatccatca tgcatagaag aacattcctg cgctctacgg cctttcttgg cgccgccgtg
     3001 gttgtaaatc cggtacaatt ttgtacgccc tcttcatcaa agctgcccgg ccaaaaggct
     3061 gtcgggttac aggtttacac cctgcgcgat ctgatggcca aagacccgga aggcacgctc
     3121 aaagcggtag cagatttggg ttacaactac attgagttgt ttaactatcg cgaaggtatg
     3181 tacttcggca agacggtgaa ggagatgcag gagtttcttc aaggcatcca attgcccgtc
     3241 ttcagcagcc acataccgac gggcgcgcnn nnnnnnnnnn nnnnnnnnnn caccgtcctc
     3301 aatgatgtgg aacgcgccgt agccgatgcc aaaatgctgg ggcaggaata cttggtttgc
     3361 ccctggctgg ccgactcgga gcgcaaaacc atagacgatt acaagcgtct gtcggtggca
     3421 ttcaacaaag ccggcgaagt gtgtaagcaa tatggcattc agttttgcta ccacaatcac
     3481 gattttgagt ttttcaagct ggacggccag attccaatgg atgtaatcct cgccgaaacc
     3541 gacgctaagc tggtacagat agagcttgat atttactgga tcaccaaggc gggcttcagc
     3601 tatgcggact acttcaagcg ctacccgggc cggttcccgc tgtggcacgt caaagacatg
     3661 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnngtagaac gagatgtgtg taagcccccg cctttggaaa
     3781 gcgtcaaaat gagccgtgag tacctgcgag ggatactttg agagcctttt cctcacaaat
     3841 aatcattcta ccaatgaaaa catactattc ctttgctctt gcattggccg ccatcctcat
     3901 gttggtcgcc tgccaaccca ccgacaacaa aagcaccgcg acatcccccg gcagtgctga
     3961 aacagcagcc tccaagcccg ccaacgctcc tgcctaccgg catctgaaag gccaggtagg
     4021 caccctcgcg gtagtgatgg acctcgcgta ccggcctgcg ggcgacgatg agttctccac
     4081 cgatatgtat cacgggagct attattacag cagcaaagaa aacccgatag acctctacgg
     4141 cagcgttgat tctaccggcc aattggtact gtacgaagtg acgctcaacg atgatacctc
     4201 gcccacgttt accggctcct tcgatcagga acagggcgtc ttcaaaggcg aatggagcag
     4261 cggcgacggc aagaaaaaaa tgcccgtgga actgcacgaa aactatgatg atgccgttcg
     4321 gttcacgact tatgagctgg aagcaagcca ggatttattt gaaggcaaag aaaactcgcc
     4381 ttatgccacc atttcaaaaa tatggctcca gcctgctgcc ggaacggatg caaccctggc
     4441 ctcctttttg aaaaaaatca tccatcaggg tttggtcggc gactcattgg caacgctgta
     4501 tccgatacag gaaaaagcct tcgaggagct cagtaaagac tactttgcat cttaccgaac
     4561 ggacatgggc gacgtgaaag aggaagaact tggtcccgac gattccttta tgtttaccta
     4621 cgaacaaaat gcttccgtag aggtgctgta caatcaaaac caactgctca cgctgggcta
     4681 ctgggattac tggtacaccg gcggagcgca cggcaactac ggtacctggc tcgcttctta
     4741 cgatctggcg gcccaaaaag agatcaccct ccaggatgta tttcnnnnnn nnnnaaaata
     4801 acgccggcat tggaaaaggc agtgcgcagt cggtttggta tctcggcaaa agaaccgctg
     4861 agttccgttt tgtttgaaga tgccattgcg gccaccgaaa acttcggcct caccggcaaa
     4921 ggcgtcattt tcaactaccc gccgtatgaa attgccgcgt atgcagcggg cgagattcgc
     4981 ctgtttgtgc cgtattcgga aatcaagaac ttgttgcagc cggcctttgc ggcaaagtat
     5041 gtgaagtaga gcctttatat ttctttccgt atttatttag cattggctgt taccacgtgc
     5101 gccacggcgc ggcagcagct tgtggctttg gaaaaggtgc taagaagtaa cttctttcct
     5161 acatttgtgc cctcaaaaca aaaaatacca tgcctacctg gttgtcagtc atattggaaa
     5221 tcatcaaaat cagcgtgcct gcgctcatcg tgttctttac ggtacacacc ctgctgaagc
     5281 aatacttggc cgggcaggag cgcatgaaag cgttggaact tcagcaaaat cgcctgggtt
     5341 ctaccacgcc cctgcgattt caggcgtatg agcggctgtc actgctttgc gagcgcattg
     5401 ccctgccgaa tctcatcatg cgtatgcgcc ccgaaaccgc caacgtggcc gagtaccgct
     5461 tggccctgat gctggccatc caacaggaat ttgagcacaa catcacccag caggtgtatg
     5521 tgtcggaaca attgtgggaa atcatcaaaa tggcccgcga ccacaccgtg gaagtcatca
     5581 acatcagcgc tgaaaagttg ccgcccaaat ccgaggcgaa agaactggcc cagtttatcc
     5641 tcagcctccc gccggaaaat actacgggcg ctttagacaa ggcactggcg gccatcaaac
     5701 gcgaggcggc tgcgatgttg taacagagtg cctccctgaa aaagtattaa tatatgtaaa
     5761 tcacctgttt ggacttcgat aagtaggctc tacaaacaaa acatctcatc ctatcgctgc
     5821 accaccaagc gggccaccct gcgctctgcc ccgacctgca catacagact gtaccagccg
     5881 ggcggcagca tgccgacgag caactcggta ctcccagaaa agaaagcggc ccgggtatgc
     5941 aacacctctt tgcccgcgct gttgcgcacc gttatcatac ccgcagctac gggcgcgcct
     6001 tcccactcta ccgtaacggc cattgctgcg gggttggggt acagatgcag cccggcctct
     6061 acgggcagcg ttggggcagt acccgatacc ggcccgcagg gcgctaccgg ctcgccgagt
     6121 gcgccgagac gataattggg aaaagtaggc gccccgccgc cccgcatccc ccagggcagt
     6181 tgtacatcgt tttgtaccac ctcgcaggcc aggcccggca ggtccggctc ccggatcaca
     6241 tggatggtgg tgcaactgga acaaaacatg tacagcttgc agtccgggcc taattgcatc
     6301 atataaaaag tagtcggtat cggatcggca tagccgtcgt attcggccac caatacccgg
     6361 cttgcggcaa cgtctggcgc ccacaggtcg tactggtaaa gtactagatt aatactggca
     6421 tacacgaaac gtccacttgg agaaaaacct acgcccgcaa gccaacctga agccggttga
     6481 atgtaaaaat agcgagggtt gctaaactcc cctgtgctgc ggtcaaaatc gtagagttgc
     6541 acgcccagat cattggtgca atgggcgaaa agtgtaccgt cgggcgaaaa agtaatttgg
     6601 gaaaaaaatg cgcccgttct atcctgacct atgtattgtt cagtaaggga cactacgccc
     6661 atatcggtaa gcaggatttt gtggtagccg ttgccccaga gatacgggga tattatccac
     6721 caatcctgcc cattggtgtg cctgaccgca gtaagattca taataggggt aaaatcattc
     6781 gagtaatcaa taatcctcac gtttttttct ataaccgcac ctagacctcc attcccgtac
     6841 atgtcaattt tagagtaatg caaaatgcaa aacaccccat aagagcttcc aggagggtat
     6901 tcatttgcaa tatgaaagag ataataatag cggtcactcc cgggcatggg taccgccata
     6961 caactctgtg ggcccgctgt atagccaggg gtaattcgcg gtggataaca aacataatca
     7021 tgaaccgcac cggcattaat atcatgcccg ttttccatca ggttgaaaga ggcatcgtgt
     7081 atttcacagc cattggtgaa gaaaatgagt tctccgtctg catcactcat ttgcgctacg
     7141 ctgctccatg cggtaatcag aacattgtcg ggagcgctga tggtaggagg aatggtattg
     7201 aaattcattc gggcagcttc tccaaatccg ttgttcggat agaattcttc aatgaatagc
     7261 cagttatagt cgtgtttttg ggcgctcaaa tgagtggaga tgataaaaag cacgggtatg
     7321 aaaatggctt tcaatttcat ggctgctttg ttttttatga taaagaataa gtgcttcctg
     7381 cgcggtcagg acttccggcg caggaagcag cataaaaaaa ctgataacgt tatcgttgaa
     7441 ttaccaagcg ggccactttt ctctcagcgc cggctcgcaa ctgcaaagtg taatatccgt
     7501 tgggcagttg ctgcgtttgt atttcacatc ttccgccgta cagtggtatg cggctctgcc
     7561 ataccggcgc gcccggctgg gaatagataa aggtacttca taacggtaag gatttaaagg
     7621 gttaagaaaa agagtctatt gatcgttaca aaagcacaaa aaaggattta agtaaaaaat
     7681 actatttctt gattttctga aaatttaaca ttaataatgc aaaatatggg cagtcttaaa
     7741 atataggcaa atcaaatatt gtgaggaagc ccgcccttgc cagaccgaga gagggcttct
     7801 cagccgcgcc atccggcaaa gagaagccct ctcttacaga tcactcactg ccccgtaaaa
     7861 aacaccgcat tgctgaacaa cagcttgccg ttttcccaga agccccgaaa cagcggattg
     7921 tccaccatat atattactgc gccgcgccct ttgtcttcta cggcaaagac ggcagagttt
     7981 ttcacctgct gctgagcata ttggcccacg aatccatagt agttcgggtt gtccccgata
     8041 gagcctacat tccaggcgcc tttgagccag ggataagcgc ccgctccggt tttgagtgaa
     8101 aaatagtgtt cgcccatgcc gaatgccagc gggtgcgtga tgtccatttt tactttaaaa
     8161 atagctccgg gaatctctcc gctgatggcc ctgcgctcct ggtctccgaa gttgagcagg
     8221 cggctgtcca attcagcttt ttccgcctcc ctttccgctt tgttttctgc gtctttgctc
     8281 tcgtagcgag tcagggcaaa accgctttta ccctggaggg agttcaatgc gtagccaacg
     8341 gcaatgaggc ggccgccttt ggaaatccat tggctcaagc ggccggcagc gccttcgtcc
     8401 aaaccgtaac cgccatcggc catgatgagg aggtcgtaat tgctcaggtc caacgagccg
     8461 agattattcg ccggcaaagc ggcaaacgga tagccgaggt ctttttccaa ataataccaa
     8521 acctggccat aagaattggc agaagtgccc tcgcccgaca gcacggcaat gcgtggccgg
     8581 gcagccatag gtacggcatc ggaaccaaaa tcgaagcctt tttctaccat gccgctgcgc
     8641 acgattgccg catccacctt gtgctttttg atggcctccg atacggtttg attcaaaaaa
     8701 tcagcagcca gacgattgtc ggcacgggtg atgatgagcg taccggcggc atagtcctgg
     8761 ccttctattc ggaacggaga acgcgcaaat cgaacgccga tgccctgttg cgacacatcg
     8821 gccagaaaac gggcggcgtt catggagttc cattttacca gatacgcata cggcgcagcg
     8881 cctgccggca aagcagccgg agccggcaat gcaaaagcgc cctgcggttc gaggcgttct
     8941 tcgagggcgt aggcttccag tccgtgcgcg tagggcagcg accaggcggt gatgtcgtaa
     9001 gtgacggaat cggccaattg cgtttgcggc tccatcagga tttgcgccat ggttccggca
     9061 ggctggtaag cgctgatgac gaggtcgttt tcctctattt taaaggcagt tccggcagcg
     9121 cctttgatat aatcgaaacc tttgagcgag gcgtttttgc ccgctttgcc gtagcgaatg
     9181 ccgttgcggt cgagcagttc ggcgaaagcc gccaatttac cggcagggtt ggaggctttg
     9241 ataatgaagg ctttgtaacg ccccggcggg ttggtgcggc tgcggtcgta gtgcgcctgg
     9301 aaatttttca ccagatcggc tacatgctgc gaggccactt cgacggtaga gagcgctgcc
     9361 gtgcgatggt gcgtcacgcg gtcgcgcagg gtgagggtgt ctccgttttc gatgatgagt
     9421 gcgcgcccgg ccccgatgcc gccctgctca
//
LOCUS       DAORLF010000149         9332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3981_length_9332_cov_1.956020, whole genome shotgun sequence.
ACCESSION   DAORLF010000149 DAORLF010000000
VERSION     DAORLF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3981_length_9332_cov_1.956020"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK198_12795"
     CDS             <1..282
                     /locus_tag="PK198_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39659.1"
                     /translation="KVISLRTPHLVEDSGALFQESEIINISFQQGPMEVTDAGRHRRY
                     LPALHRVRSSFEQAFGGTVRLFDAHSLAPIPADADSCASTRYVVMAQKK"
     gene            585..2132
                     /locus_tag="PK198_12800"
     CDS             585..2132
                     /locus_tag="PK198_12800"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39660.1"
                     /translation="MALQNIPIGLQDFRKIIENDFLYVDKTEYIYKMASKPGAYFLSR
                     PRRFGKSITIAVLQELYLGSRELFKGLWIEDKWDWGKKHPVIRISFKDINFEQRGLES
                     PLAERMKEIAAQYGVELKTPTARDRFRELATVLAATAKVVVLIDEYDAPITHYLGKEL
                     PQAIENRDFLRGFYSVLKEADHLLEFVFLTGVSKFSKVGIFSGLNNLLDITFHPEFAT
                     MLGYTQEELEYNFENHIAATAAKMSLSKEAFLAKLKEWYNGYRFHHLAETVYNPVSLN
                     SFFNVGDFENFWFETGTPTFLINILREQGLYKFDLEPKSQIEFGSFELDNLYSYGLLY
                     QTGYLTIQYRDEYGLYHLDYPNYEVRNSMLAYLLDAFGGVHRGGGIVLAIKMEQAFMA
                     HDIAKVMHILQGLFKGIPYQLYEKYPESFYHAATHLIFAYMGLRVHSEVCTSDDRADA
                     VVETPDKVYVLEFKLDESAEAALEQIRQKRYHEAFWNLGKPVVGIGINFSSKTRNVEE
                     WKVEEMA"
     gene            2205..>2504
                     /locus_tag="PK198_12805"
     CDS             2205..>2504
                     /locus_tag="PK198_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39661.1"
                     /translation="MRAFLLLLCVVGSVSAAAQALLHSRQGSASAYVYQISNAQAEKV
                     CRAKIWQADSTFFHTLRDSFPADSSLQRALPQGHYLIARIVKNQLKIDLLSVQPFD"
     assembly_gap    2505..2669
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2670..>6225
                     /locus_tag="PK198_12810"
     CDS             <2670..>6225
                     /locus_tag="PK198_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39662.1"
                     /translation="HGGLAVFYDLSRQRNNPWTRRLVNNTPLRYVYRPVEFVLRTPVD
                     ALLSLADGKRRYRPRRVGRFLADIGRCVACIFDDYQCRRDREERFSERWRGYMVFNKP
                     KFMPGDTVRLKAFVTNRKGRPLKDSVSVMMYPEWDRPVNLGRIAPYLPGSYDFEFALH
                     DSLKLQLDRKIRVELQKKPRFGYMESVFRYEDYELKSLQFSLRADGDYQYRGKPYRLH
                     LKGTDENELTLPDARTELLLTTVQVLEFKDSVVFVPDTLWRKDLALEPGGETAVEIPD
                     SIFPLCDVRYRLEAVLLSADNERVSKSQNVTFFHTHKELHLDLQKDSLRMEYREEGRQ
                     KPALATLTLLDALGQALEERLVQLPHTEAVNAYVSEYKLDMGLLKKTWRPASEQALLQ
                     CFASRSHDSLFIACDNPRRIPFRYFLYRHNREIERGYGTDWQLRRPAGERETYFIALQ
                     YLWGGQVRSENYSVDFARRQLNVQVEQPALAWPGMETDITVTVTDQRGRPVPNADLTA
                     WAVTQKFQPQAPALPWFEKQTPQRRLINTFNALRDQPQNNRTPLNFAYWRSKTGLDTA
                     EYYRFAYPGRNLYRFEYDAPDSIAQFAPFFVLNGAPQPVHVVYVDQKPAYFGWNTNRQ
                     PYSIRISPGYHRVELRCTDRLIHLDSVLISPGKKLILSLSDSIQDQHISTTSMPTSLT
                     QDEKNNLYRYIFPFRYKDNAWDYIEQKGAIQLLQPASGTFGRQLTGPIMPDVVQYRNL
                     RDNHKIDFAHEPMMEYDFAPGLLKMRSVNPEWHYPDKLSGAAREEWGDLVWKKEDLLP
                     KFKSKQWSEYWYINLAPYTITLSGRRGRLQLDGNFTNKYPDKAYVLLFKYENPDFVLI
                     QSAYYKLFHNLGPGNYRAIWLLQDGRYFVKDSIGIEANGINYLRIDSLDILPADDFSK
                     SCKRSIERDYIQRWSLLEVRENYIGPTQFTGPERLFIGSIKDDKGEPLIGAAIKIKGQ
                     ARGTVTDFDGQYSLLAPEDAVFLISYLGFESMEVRPSAQSDFVLHSADYMLSEVLVSA
                     YKSDLVQQDRVSTGYSVSLSNRKGRDFSSKLAGIQVRGSRSDATEYYIDGVRVSGELL
                     GLSSDQIQNIEVISDSAALAVFGPGAAEVVLITTKAGAKGMAFDAAFMEGAAEASSIR
                     SRFSDYAFWQPRLRTNAEGKATFRVSFPDDITQWRTFVP"
     assembly_gap    6226..6354
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6355..8013
                     /locus_tag="PK198_12815"
     CDS             <6355..8013
                     /locus_tag="PK198_12815"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39663.1"
                     /translation="ALNYASDTIDIEAAFEINGAAAGRSKHRLADAATDSVWLSPANT
                     DSLTVQFSIRKSDGYFDGERRSIPVFPKGIERSEGQFFVLEGDTSIIIQLPEKQGPVQ
                     LYARADLLDVLREDLSWLLRYEYDCNEQMASKLKALLADEQIAAWLEQPFRHKQELQK
                     LIHRIEKNRNERGLWGWWNKSATSGWISVHILETLAQARSKGYKVEALPRKAVEEIIW
                     ELENRQTGNERKLELLAMLSAFGEKIDYSAYLPFIQKDTLLNVYNHFRLIELQQRHGL
                     PWQRDTLLRYQRETMFGNVFFSDSASLEQPWRGHLPLTLMAARILHSDSTASPDLQRR
                     IRNYLLEQRQSGLWWNTFQRAQWLEFFMETWSKNAKALQAPRLELSGAVQDSATQFPY
                     ERVLPPGGRIELRKTGDFPVYFTAYQRYWDNDTRADSTYFKVRSFFDTLPGDTLQAGV
                     PAKLVTEVTVFRDAPYLMLEVPIPASCSYESKRTNYGKESHREYFKHKTAIFCERLGI
                     GTHRFEVELIPRYAGSYQLNPAKVELMYFPVFQANVAGIRVRVR"
     assembly_gap    8039..8070
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8215..8706
                     /locus_tag="PK198_12820"
     CDS             8215..8706
                     /locus_tag="PK198_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF39664.1"
                     /translation="MSKQATSDEQQFLELLETHQNIVHKVCRMYARSEEARRDLFQDI
                     AAQMWRAWPAFRQDCKVSTWMYRIALNVAISGVRKQELQTTELTEIQHFVSADEQETP
                     DAYARLYAALERLSPSEKSVALLYLEDHSYEEMAQITGLSADNLRVRMFRIREKLKTI
                     LQS"
     gene            8717..>9332
                     /locus_tag="PK198_12825"
     CDS             8717..>9332
                     /locus_tag="PK198_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39665.1"
                     /translation="MNNDALDNLKSDWQNAAGSPNPIDKEQLLLMLKKRNRSIFRSMY
                     RNLMAEVVLGGLALLVWVYFVSRIPSNNGEAYLAAIQLTILGVLPLVGFYVAGFRHLG
                     RGMASDSRLMPALQQTIAYWDQLLKLYFWGGATLMPAFLLSAAWLVNSLTYQSLFRIT
                     EYMSWQKILGWTVFISIFAMVFVWFSIKISYGKHVKQLKENLREL"
BASE COUNT         2161 a         2552 c         2322 g         1971 t
ORIGIN      
        1 aaggtcatct ccctccgcac gccccacctc gtcgaagaca gcggcgcact cttccaggaa
       61 agcgaaatca tcaacatcag cttccagcaa ggccccatgg aagtaaccga cgcaggtcgc
      121 caccgacgct atctgccggc cctgcaccgc gtgcgcagca gcttcgagca ggcattcggc
      181 ggcacagtac gcctcttcga cgcccactcc ctcgcaccta tccccgccga tgctgacagt
      241 tgtgcctcca cccgctatgt tgtcatggct cagaaaaaat aaatagctga tctctaagtg
      301 attatttggg cggctgcccg ctccggcacc acccccagcc cctccaaagg agggtagccg
      361 cagacagcgg gcaccgggcc gtccgctcca tgcgttgccg ctccgcagcg ccgggtaaaa
      421 accacggcgc tgcgcaacgg caacgcatcc gcttccgtcc ctgccgcaac cacccacaga
      481 gccatcttgt ggcaaggggg ctttgcctca ctacatcctt gcaaacacat caagcgatga
      541 cctttgcaaa atatacttat ctttgatctc aaatagcgca caaaatggca cttcaaaata
      601 tccctattgg tttacaggat ttcagaaaaa tcattgagaa tgatttcctg tatgtggaca
      661 agacggaata catatataaa atggcgtcta agccgggcgc atacttcctt tcccgcccgc
      721 gccgcttcgg caagtccatc accattgccg ttttgcagga attgtacctc ggtagccggg
      781 agttgttcaa agggttgtgg atcgaggata aatgggattg ggggaaaaag catccggtta
      841 ttcggatttc tttcaaagac atcaattttg agcagcgcgg gctggagtca ccgctggcag
      901 aacggatgaa ggaaattgcg gctcaatatg gcgtggagtt aaaaacgcca accgctcggg
      961 accgcttccg ggaattggcg acggtgctgg ccgccactgc caaagtcgtc gtcctcattg
     1021 acgaatacga tgcgcccatc acgcattacc tcggcaagga actgcctcag gccatcgaaa
     1081 accgcgactt cctgcgcggg ttctactccg tactcaaaga agccgaccac ctgctggagt
     1141 tcgttttcct caccggcgtc agcaagtttt ccaaagtggg catcttctcc ggcctcaaca
     1201 acctgttgga cattaccttc cacccggagt ttgccactat gctgggctat acgcaggaag
     1261 agttggaata caattttgaa aaccacatcg ccgccaccgc cgcaaagatg tctctgagca
     1321 aggaggcatt tctggcaaag ctgaaagagt ggtacaacgg gtaccggttt catcatctgg
     1381 ccgagaccgt ctataaccct gtttcactca actccttttt caacgtcggc gactttgaaa
     1441 acttctggtt tgaaaccggc acgcccactt ttctcatcaa tatcctgcgc gaacagggct
     1501 tgtataaatt cgacctggaa cccaaatccc agatcgagtt cggcagcttt gaattggata
     1561 acctctactc ctacggactg ctgtaccaaa cgggctatct caccatccag taccgcgatg
     1621 aatacggcct ctaccatctc gattatccca actacgaggt ccgaaactcc atgctggctt
     1681 acctgctcga cgctttcggc ggagtacacc ggggcggtgg cattgtgctg gccatcaaga
     1741 tggaacaggc attcatggcg cacgacattg ccaaagtcat gcacattttg caggggctgt
     1801 tcaaagggat tccttaccag ttgtacgaaa agtacccgga gagcttttac catgccgcca
     1861 cccacctcat cttcgcctac atgggtttgc gcgtccacag cgaggtatgc acctccgacg
     1921 atcgcgccga tgccgtagtg gaaacgcccg acaaggtgta tgtgctggag tttaaattgg
     1981 acgaaagtgc agaggctgcc ttggagcaaa tccgccaaaa acgctaccac gaagccttct
     2041 ggaacctcgg caaaccggtc gtcggcatcg gcatcaactt cagcagcaaa acgcggaatg
     2101 tggaggagtg gaaggtggaa gagatggcct aatggccgac aaaacgctac aaagccccgc
     2161 ctcgcttcgt tttaacagca tacattgcca taaaaacaag cgccatgaga gctttcctcc
     2221 tcctgttatg cgtcgtcggc agcgtgtcgg cggcagcaca ggccctcctc cacagccgtc
     2281 agggcagcgc ctcggcctat gtgtatcaga tcagcaatgc gcaggccgaa aaggtgtgca
     2341 gggccaaaat ctggcaggcc gattccactt tttttcacac cctgcgagac tcctttcccg
     2401 ccgacagcag cctgcaacgc gccttgccgc agggccatta tctcattgcc cgcatcgtca
     2461 aaaaccaact caaaattgac ctcctcagcg tgcagccctt cgacnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cacgggggct tggccgtttt ttacgacttg
     2701 agccgccaga gaaacaaccc ctggaccagg cgtttggtca acaacacgcc gttgcgctat
     2761 gtgtatcgcc cggtggagtt tgtgctgcgc acgccggtgg acgcgctgct ttcgctggcc
     2821 gatggaaaaa ggcggtaccg gcctcgtcgg gtggggcgtt ttttggcaga catcggccgc
     2881 tgcgtagctt gtatttttga tgactaccaa tgccggcgcg accgcgaaga gcgtttcagc
     2941 gagcgttggc gaggttatat ggtattcaac aagcccaagt ttatgccggg cgataccgtt
     3001 cggctaaaag cctttgtgac caatagaaaa ggccgccctc tgaaggattc cgtttctgtg
     3061 atgatgtatc ccgaatggga tcggcctgtg aatctgggcc gcattgctcc ctatttgccg
     3121 ggcagctatg attttgaatt tgccctgcac gacagcctga aactccagtt ggacagaaag
     3181 atcagggtgg aattgcaaaa aaaaccgcgt ttcggctata tggaatcagt ctttcgatac
     3241 gaagactacg aactcaaaag tctgcaattc agcctgcggg ccgacggcga ttaccaatac
     3301 cggggcaaac cctaccgcct gcacctgaaa ggaaccgacg aaaacgaact gaccctgccc
     3361 gatgcccgca cggaactgct gctgacgacg gtgcaagtgc ttgaatttaa agacagtgtg
     3421 gtttttgtac ccgacaccct ctggcgaaag gacctcgctc tcgaacccgg tggcgaaact
     3481 gctgtcgaaa tccccgattc cattttcccg ctctgcgatg tgaggtatcg cttagaggcc
     3541 gttttgctgt ctgccgataa cgagcgggta agcaaatccc aaaacgtcac cttttttcat
     3601 acccacaaag agcttcattt ggacctccaa aaagacagcc tccgcatgga gtaccgcgaa
     3661 gaaggccgcc agaaacccgc tctggccacc ctcacgctgt tggacgcgct ggggcaagcc
     3721 ttagaggagc gcttggtgca actaccccac acggaggccg tgaacgctta tgtatcggaa
     3781 tacaaattgg acatgggcct gctgaaaaaa acctggcggc ccgcctccga acaagcgctc
     3841 ctgcaatgct ttgcctcccg cagccacgac tcgctgttca ttgcctgcga caatccccgg
     3901 cgcattccct tccgctactt tttgtaccgc cacaaccgcg aaatagagcg cggctacggt
     3961 accgactggc aattgcgcag gcccgccggc gagcgggaaa cgtacttcat cgcgctgcaa
     4021 tacctctggg gcggacaggt gcgcagcgaa aactactccg tggacttcgc ccgccgtcaa
     4081 ctcaacgtgc aggtggagca gccggcattg gcttggcccg gcatggaaac cgacatcacc
     4141 gtgaccgtga ccgaccagcg cggcaggccc gttcccaatg ccgacctcac cgcctgggcc
     4201 gtgacgcaaa aatttcagcc tcaggcgcct gcattgcctt ggtttgaaaa acagacgccc
     4261 caacgcaggc tcatcaatac gttcaacgcc ctgcgagatc agcctcaaaa caatcgaacc
     4321 ccgctcaact ttgcctattg gcgcagcaaa accggcctgg acacagccga gtactaccgc
     4381 tttgcctacc ccggccgaaa cctctaccgc ttcgagtacg acgcccccga cagcatcgcg
     4441 cagttcgcgc cgtttttcgt actcaatggc gcgccgcagc ccgtccacgt cgtgtatgtg
     4501 gaccaaaaac ccgcgtattt cggatggaac accaaccggc agccctactc cattcgaatc
     4561 agtccgggct atcaccgggt agaactgcgc tgcaccgacc ggctcattca tttggacagc
     4621 gtactcatct cgccgggtaa gaagctgatt ctgagtcttt cagattccat tcaagaccaa
     4681 cacatcagca ctacttccat gcccacctcc ctgacgcagg atgaaaaaaa caacttgtat
     4741 cggtatattt ttccttttcg atacaaagac aatgcatggg attacattga gcaaaaagga
     4801 gcaatacaat tgttgcaacc agcttccggc actttcggca ggcagttgac cgggccgatt
     4861 atgcccgatg ttgtgcagta ccggaatcta cgggacaacc ataaaataga tttcgcccac
     4921 gagccgatga tggaatacga ttttgcgccc ggcctgttga aaatgcgcag cgtgaaccca
     4981 gagtggcatt atccagataa actgagtggc gcggcgagag aggaatgggg agacttggta
     5041 tggaaaaaag aggacttgtt gcctaagttc aaatctaagc aatggtctga atattggtac
     5101 atcaatttgg caccgtacac aattaccttg tcgggccgtc gtgggcgctt gcaattggat
     5161 ggaaatttca caaataaata ccccgataaa gcctatgtgc tgttgtttaa atatgaaaat
     5221 cccgactttg tactgataca gtctgcctat tataaacttt ttcacaacct cggtccaggc
     5281 aattaccggg caatatggct gcttcaggat gggcggtatt tcgtgaaaga ttccatcgga
     5341 atagaagcca acggcatcaa ttacctgcgt atagacagtt tggacatttt gcctgccgat
     5401 gattttagta aaagctgtaa gcgaagcatc gaaagagatt atatccaacg atggtcacta
     5461 cttgaggtaa gggaaaatta cattggcccc acccaattta ccggaccaga acgtttgttc
     5521 atcgggagta ttaaggacga caaaggtgag ccgcttattg gcgctgccat caaaataaaa
     5581 gggcaggcgc ggggtacagt aaccgatttc gacgggcaat acagtctttt agctccggaa
     5641 gatgccgtgt tcctcatttc ttatttgggt tttgaatcta tggaagtccg accttcggct
     5701 cagagtgatt ttgttttaca cagtgcagat tatatgctat ctgaagtgct tgtaagtgct
     5761 tataagtccg accttgttca acaagatcgc gtttcaaccg ggtattcagt aagtctttcc
     5821 aatcgcaaag gcagggattt ttctagcaag ttggccggta ttcaggttcg cggcagccgc
     5881 tccgacgcca ccgaatacta catagacggc gtgcgggtat cgggcgagtt gttgggcttg
     5941 agcagcgatc agatacaaaa catcgaagtc atttccgatt cggcggccct ggcagttttt
     6001 ggtccgggcg cggcggaggt cgtactcatc actaccaagg caggagccaa aggcatggct
     6061 ttcgatgcgg cgtttatgga aggcgctgcc gaggcaagtt ccattcgcag ccgtttttca
     6121 gattacgcct tttggcaacc ccgcctgcgc accaatgccg agggcaaggc cacttttcgc
     6181 gtcagcttcc ccgacgacat cacgcagtgg cgcaccttcg tacctnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgctc
     6361 aactacgcct ccgacaccat agatatagag gccgcttttg agatcaacgg cgcggcggcg
     6421 ggccggagca agcatcgctt ggccgatgct gccaccgatt ccgtgtggtt gtcgccggcc
     6481 aataccgatt cgctcaccgt gcagttttct atccgaaaat cagacggcta cttcgacggc
     6541 gagcggcgtt ccattcccgt ttttcccaaa ggcatcgagc gcagtgaagg gcagtttttt
     6601 gtcctcgaag gagacacctc catcatcatc caactgcctg aaaaacaagg ccccgtgcaa
     6661 ctctacgccc gcgccgacct gctcgatgtc ttgcgcgaag acctctcctg gctcctgcgc
     6721 tacgagtacg attgcaacga acaaatggcc tccaagctca aagctttgct ggccgacgag
     6781 caaatagccg cctggttgga acagccattc cgacacaaac aggagctgca aaaactcatc
     6841 catcgcattg agaaaaaccg caacgaacgc ggcctctggg gctggtggaa caaatcggcc
     6901 acctccggtt ggatcagcgt acacatattg gaaacgctgg cgcaggcccg cagcaagggg
     6961 tacaaagtgg aggcgcttcc gcgcaaagcc gtcgaggaaa tcatctggga attggaaaac
     7021 cgccaaacgg gcaacgaacg caaattggaa ctgctggcca tgctgagcgc tttcggagag
     7081 aaaattgatt acagcgccta tctgcccttt atccaaaaag acacgctgct gaatgtgtat
     7141 aatcacttcc ggctcatcga gttgcaacaa cggcacggcc tgccctggca acgcgatacg
     7201 ctgttgcgct accaacgcga aaccatgttc ggcaatgtgt ttttcagcga ttctgcttct
     7261 ctggagcaac cctggcgcgg gcatctgccc ctcacgctca tggccgcccg catcctgcac
     7321 agcgattcca ctgcctcgcc cgatctgcaa cgccgcatcc gaaactacct gctggaacaa
     7381 cgccaatccg gcctgtggtg gaataccttt cagcgggcgc agtggctgga gttttttatg
     7441 gaaacctgga gcaaaaatgc caaagccctg caagcccccc gtctggaact ctccggcgcc
     7501 gtgcaagaca gcgcaacgca attcccctac gaacgggtat tgccgccggg cggccggatc
     7561 gaactgcgca aaaccggtga tttcccggtg tatttcactg cttatcagcg ctattgggac
     7621 aacgacaccc gcgccgattc gacgtatttc aaagtgcgct cgtttttcga tacactgccg
     7681 ggcgatactt tgcaggccgg tgtgccggcg aaattggtaa cagaggtgac ggttttccga
     7741 gatgcgccat acctgatgct ggaggtgccg attcctgcca gctgttcgta tgaatccaaa
     7801 cgcaccaact acggcaaaga gtcgcatcgg gagtatttca aacacaaaac ggccattttt
     7861 tgcgagcggc tcggcatcgg cacgcatcgc ttcgaggtgg aattgattcc gcgctacgcg
     7921 ggcagttacc agctcaatcc ggccaaagta gagttgatgt attttcctgt ttttcaggcg
     7981 aatgtggcag ggataagggt gcgggtgcgg tgaaagggtg ggcagcaggc ggtgtgcann
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtaggcggt cctcggtagg cggtatgcag
     8101 tgctaagcgc gcaggtaccg cccaccgccc actgcctacc gcctactttt tttctcatcc
     8161 gtgtaacatt tcccttcgtt ccaacgacca atacggcaaa atcaatcaaa acaagtgtca
     8221 aaacaggcga catccgacga acaacaattt ctggaactgc tcgaaacgca tcagaacatc
     8281 gtacacaaag tgtgccgcat gtacgcccgc tcggaggaag cccgccgcga cttgtttcag
     8341 gacatcgcag cgcagatgtg gcgcgcctgg cccgcctttc ggcaagactg caaagtaagc
     8401 acctggatgt atcgcattgc attgaatgtg gccatatccg gcgtgcgaaa acaagaatta
     8461 caaactacgg aactgacaga aatacagcat tttgtatccg ccgacgagca ggagactccc
     8521 gatgcgtatg cccggctgta tgctgctttg gaacgcctgt cgccgagtga aaaatcggtg
     8581 gcgctgctgt atttggaaga ccattcttat gaagaaatgg ctcagatcac cggcctgagc
     8641 gccgacaacc tgcgggtgcg gatgtttcgc attcgcgaaa aattgaaaac tattttgcaa
     8701 tcctaaacaa aaagacatga acaatgacgc attggacaac ctgaaatcgg actggcaaaa
     8761 cgccgccggc tcgcccaatc cgatagacaa agagcaactg cttttgatgc tcaaaaaacg
     8821 caatcgctcc attttcagga gcatgtaccg caacctgatg gcggaggtcg ttttgggcgg
     8881 attggctttg ctggtctggg tatattttgt gagccgcatc ccgtcaaaca atggcgaggc
     8941 gtacttggcg gctattcaac tgaccatatt gggcgtgctg cctttggtcg ggttttacgt
     9001 cgccggtttc aggcatttgg ggcggggcat ggcctccgac tcccggctga tgcccgcgct
     9061 gcaacaaaca atcgcctatt gggaccaact gttgaagctc tatttttggg gcggagccac
     9121 gctgatgccg gctttcctgc tgtcggcagc ttggttggtc aacagtctga cataccaatc
     9181 cttgttcaga ataacggagt acatgtcttg gcagaaaata ctcggctgga cggtgttcat
     9241 ctctatattc gcgatggttt ttgtgtggtt ttcgatcaaa atcagctacg gcaaacatgt
     9301 aaaacaactg aaggaaaacc tgcgggaact gg
//
LOCUS       DAORLF010000150         9310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_3993_length_9310_cov_2.467963, whole genome shotgun sequence.
ACCESSION   DAORLF010000150 DAORLF010000000
VERSION     DAORLF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3993_length_9310_cov_2.467963"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="PK198_12830"
     CDS             complement(<1..1492)
                     /locus_tag="PK198_12830"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRF39666.1"
                     /translation="MKYFFFLLLFLISPAELPAQAPSPIRLVRNGRSPYAIVIPVQAS
                     PAELRAADFLQSHLKQIAGCTLTIRRSNVAPGKHSILIAQTPELATPDAYRIRAGQGR
                     IHIEGGSGKGCVYAISALLEEWGVRYYSPDYVVFPQKRDLFITTAPHSETPVNTYRNV
                     HGRFGENPDYRDFHKLHNIEDMFAEGYYVHTFHRLVPWQEYFGTHPEYFAEMNGKRII
                     DQLCPSHPEVLRLVAQKLRSEIPRQPDKKVWSVSQDDNFSYCQCEHCRRIIEREQSPA
                     GPIIQLVNAVADSFPDKIISTLAYQYSRQAPAHLRPRDNVQIMLCTIELNRSQPIAED
                     PRSASFLKDMEDWGRISQHIYLWDYTVDFAHSISPFPNIHTLQPNIQLFVKNNVREHF
                     QQSNTGSGHEFSELKSYLLAKLLWNPDADVEALITEFTDGYYGAAGKWMRQYLKHLES
                     EILKTGEWLDIYGPPTNHSHSFLSADNIAAYNRYFDEAEKAVADQPR"
     gene            complement(1567..3405)
                     /locus_tag="PK198_12835"
     CDS             complement(1567..3405)
                     /locus_tag="PK198_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF39667.1"
                     /translation="MKFHHLAILFVSLLLAACQQQKPAASAPSAAPDPHSFSRPDLAR
                     AQHLDLNLLIDFDTKTLSGYARWTIENPAGASEIIFDTDNLDIQKITMGKDEKPAKFS
                     LGARDSIFGSALRIQLEPCADVLTIYYATRPDAAALGWMEPSMTAGKKLPFLFTQGQA
                     ILTRSWIPCQDTPGNRITYNAEIQTPVGMMAVMSAENPQQKSPDGKYQFRMEQPVATY
                     LIALAAGDIAFKSVGPRTGVYAEPPMLEKSAWEFAEMEKMVEAAEALYGSYVWGRYDL
                     IVLPTGFPFGGMENPRLTFATPTVVTGDRSLVSLVAHELAHSWSGNLVTNSNWNDFWL
                     NEGFTVYFERRIMEAVYGKDYTEMLAQLEYWELEEEVMLLGEDSPDTHLKLDLTGRNP
                     DDGVGDIAYQKGGFFLTMLERQTDRAKWDAFLKSWFDEHKFTSQSTESFLSYLKTNYL
                     EKYNLKVNTDEWVYAPGIPANCPRPNSVKLAAAEKAAADIARQLPDTKNWTPHEWVHF
                     VRNLPAATDVATLKKLDAQFGLSSSANAEIRCAWYASAIPKGYAAEIMPGMESFLTEV
                     GRRRFLMPVYTELNKAGLSDDARRIFQKAAPGYHAVSRNSVEALLK"
     gene            complement(3500..>4132)
                     /locus_tag="PK198_12840"
     CDS             complement(3500..>4132)
                     /locus_tag="PK198_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39668.1"
                     /translation="SKELFRRIRALKSDDATVSIHNQETPHEDELFRSKTGGFQEFYE
                     MFQIPLTEFDATGRNSIHYAMENMRPQQRNLFVHNTMCSPDDIQAAQSWSDRVFWATC
                     PNANLYIENRLPNYRFFLDAEAQMTIGTDSLTSNWQLSVLEEMKTIARFQSYVSFETL
                     LRWATLNGARALGFEADLGSIETGKTPGLNLLDIGSDLKLQAGTGVRRLV"
     assembly_gap    4133..4155
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4156..4683)
                     /locus_tag="PK198_12845"
     CDS             complement(<4156..4683)
                     /locus_tag="PK198_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39669.1"
                     /translation="MHKFSADLLFPVSAPPIPNGVVVTDEQGKILAVESREQHDPASL
                     DIRRGAIVPGFVNTHCHLELSHMKGRVDTGTGLLPFLKSVVQFRNVPEEEIQEAIRRA
                     DADMYDAGIVAVGDISNKLDTAAVKDQSRIRYYTFVEMFDFLQDSRAEQTFNQYLPVW
                     EGQSTAHGNRKSAVPH"
     gene            complement(4736..5551)
                     /locus_tag="PK198_12850"
     CDS             complement(4736..5551)
                     /locus_tag="PK198_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF39670.1"
                     /translation="MNYIELNRDSWNKRTGVHVQSDFYGVKDFLAGQSTLNSIELDLL
                     GDLQGKNLLHLQCHFGMDTLSLARLGARVTGIDLSDAAIDQARQLAEQQDIPASFICC
                     NIYDLPEHLDEQFDVVFTSYGTIGWLPDLDAWGKIVARYLKPGGLFVFAEFHPAVWMF
                     DNDFTKIEYSYFQQDAIVETEKGTYTDRDADIETTCVSWNHSLDQVFRALLHSGLRIT
                     DFREFDYSPYNCFANTVETEPGRFHIRGMEGKLPMVYALQAVRNTVPVTSVAE"
     gene            complement(5623..6051)
                     /locus_tag="PK198_12855"
     CDS             complement(5623..6051)
                     /locus_tag="PK198_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39671.1"
                     /translation="MDLVVNEWLPEYFLSEASTEEKKSLETFLNRFLERNDRLVVKEP
                     SPFLDKIYRYAKDYQRMDEIVQPIRSFIKLILLDSNRCKFLSEEEIEELPTSVLEKLE
                     VGNYGSDQYLFESAAAINGEKIIVTTDEKTHRPFRRRRLL"
     gene            complement(6053..7276)
                     /locus_tag="PK198_12860"
     CDS             complement(6053..7276)
                     /locus_tag="PK198_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39672.1"
                     /translation="MISKIHIQNFRSIGQISPIELGKITVLTGANNSGKSSILYGLMI
                     LQDFLRNPNRSLEDLFALPFINLGGFSEVKSKFDALNEYIDLGITAEQESNLGAFGVS
                     YRLFLGAGKTFIKLSSDSGPFSFEARMSIGLPYVPVTETYQKAQFKRNDTEILFGWNG
                     FTASLKPRLDVFSELEESIISALQTPVNELFATDFAPISRNFTKPYYNMVPMRGIIQT
                     EEEVATMLKSEPATLEAVNQYLEKITGRTLELPGNGTPGLFSLLARNAQSGQTNFLVN
                     EGSGTNQVVTLLAKIFQPGKQFICIDEPEIHLHPGMVRSLAASFADMVSSDPKRQFLV
                     STHSEHFVQALMAKVAAGSLRPEDVKVYHLSMTETGTAVEHQSINSKGQLEGGLSHFF
                     AEELKSVKSFFKLTD"
     gene            7448..8032
                     /locus_tag="PK198_12865"
     CDS             7448..8032
                     /locus_tag="PK198_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF39673.1"
                     /translation="MAEDTTDSPKYAEILEAARRLYWKYGIKKVSVEEICREAQVSKM
                     TFYRFFPNKIEVAKVVLEQVFDTSLRDYRQIMAQDVPYEEKVRQQLRLKFEGTEAISA
                     DLIRDLYSNEEWGLTAYLEQRTEETIREIVADFAHAQQQGWVRADIQPAFLLYLFHQM
                     PAWVTNDQLLASYPHPRDLVMEVINFFFYGILEK"
     gene            8237..>9310
                     /locus_tag="PK198_12870"
     CDS             8237..>9310
                     /locus_tag="PK198_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39674.1"
                     /translation="MFRILIIGLFLLSSTHCLAQDSTALLRDSLVFKGQLITWLNANL
                     ANELPLAAGLRYLPQLNYRLPHTVTANRTPPTAHRQPHTAPRLFDLEASANIFAAANL
                     RPFDSLNATGLIKPYRLWVRYSGPQWEIRAGLQKINFGSATLLRPLMWFDQIDPRDPI
                     QFTDGVWGLLGRYYFLNNANLWLWGLYGNHQRRGWDLAPTAKYRPEWGARFQQPLPKG
                     EMALSLHHRHADTRSLEPLLPAHAAAPETRLGFDVKMDWAVGCWLEASWTANHRNLGA
                     YRHQEMLNLGIDYTWGIGNGLYIAAEQLLVATDTRAFRLARTNSLSLLSLSYPIGFFD
                     NLGAMIYFDWSNNALYRFVNWRKQ"
BASE COUNT         2097 a         2485 c         2522 g         2183 t
ORIGIN      
        1 ggcggggctg atcggctacg gccttttctg cctcgtcgaa atagcggttg tatgcggcga
       61 tgttgtcggc ggagaggaag ctgtggctgt ggttggtggg cggcccatag atatcgagcc
      121 actcgccggt tttgaggatt tcagactcca gatgcttcag gtattggcgc atccatttgc
      181 ccgcagcgcc gtagtatcca tctgtaaatt ctgtaatgag ggcctccaca tcggcatcgg
      241 gattccagag tagtttggcc agcaggtagg acttgagttc cgaaaattca tgtcctgagc
      301 cggtgttgga ttgttggaaa tgctccctga cattgttttt cacaaacaac tgaatgttag
      361 gctgtaaagt gtggatattg ggaaacgggc tgatgctgtg cgcaaaatca acggtgtaat
      421 cccagaggta aatatgctgg ctgatgcggc cccagtcttc catgtctttg aggaaggaag
      481 cgctgcgcgg gtcttcggcg atgggctgac tgcggttcaa ctcgatggta cacagcatga
      541 tctgcacgtt gtcgcggggg cgcaggtgcg cgggcgcctg ccggctgtac tgataggcca
      601 aggtagaaat gattttgtcg ggaaaactat ctgccactgc attgaccaac tgaataatcg
      661 gccctgccgg actctgttcc cgctcgatga tccgccggca atgttcgcat tggcagtagg
      721 aaaagttgtc gtcctggctc accgaccaca cttttttgtc gggctgccgg ggtatttcgg
      781 agcgcaactt ttgcgccacg aggcgcagca cttccgggtg cgaggggcag agctggtcaa
      841 tgattcgctt gccgttcatc tcggcaaagt attcggggtg cgtgccgaag tactcctgcc
      901 agggcactaa gcggtgaaaa gtatgcacat agtagccctc tgcaaacatg tcttcgatgt
      961 tgtgcagctt gtggaagtcg cgataatcgg gattttcgcc gaaacggcca tgcacattgc
     1021 ggtaagtatt gacgggcgtt tcgctgtgcg gcgctgtggt tataaacaag tcccgctttt
     1081 gcggaaacac cacataatca gggctgtagt accgtactcc ccattcttcc agcagtgcgc
     1141 tgatggcata cacacagcct ttgccgctgc cgccctctat gtggatgcgc ccctgccctg
     1201 cgcggatgcg gtatgcatcg ggagtggcga gttcgggcgt ttgggcaatg agaatgctgt
     1261 gcttgccggg cgccacattg ctgcgacgaa tggtcaaggt gcagcctgct atctgtttca
     1321 ggtggctttg caaaaagtca gccgcgcgca gctccgccgg cgatgcctgc acgggaatga
     1381 cgatagcata agggctgcgg ccattgcgca ccaatcgaat gggagacgga gcctgtgcgg
     1441 ggagttcggc aggcgaaatc aaaaacagga ggaggaaaaa gaagtatttc atggaagaag
     1501 gtttttaagt ggatgccgga aacagaatgc gccacggaac gacgcccatt gttgcggcgg
     1561 cagcccctac ttcaacaatg cctccaccga atttcggctc acggcatggt aacccggcgc
     1621 ggccttttgg aaaatacggc gggcgtcgtc gctcaggccg gctttgttga gttcggtgta
     1681 aaccggcatc agaaagcgac ggcggcccac ttcggtcaaa aaagattcca tgcccggcat
     1741 gatttcggcg gcgtagcctt tgggaatggc cgaggcatac caggcgcagc ggatttcggc
     1801 attggccgag ctgctgaggc caaattgagc gtccaatttt ttcagagtgg ccacatcggt
     1861 agcggcgggc aggttgcgca caaaatgcac ccactcgtgc ggtgtccagt ttttggtgtc
     1921 gggtagttgg cgggcgatgt cggcggcggc tttttcggcg gcggccaatt tgacggaatt
     1981 gggtcgaggg caattggcag gaatgccggg cgcataaacc cactcgtcgg tattgacttt
     2041 gaggttgtat ttttccaaat aattggtttt caaatagcta agaaaactct cggtgctttg
     2101 cgaggtgaat ttgtgctcgt caaaccagct tttcagaaag gcatcccact tggcgcggtc
     2161 ggtctgtcgc tccagcatgg tgaggaaaaa accgcccttt tggtaggcaa tatcgcccac
     2221 gccatcatcg ggattgcggc ccgtcaggtc gagtttcagg tgcgtatcgg ggctgtcttc
     2281 gcccagcagc atcacctctt cttccaattc ccagtactcc aactgagcca gcatttcggt
     2341 atagtctttg ccatacacgg cctccatgat gcggcgctca aaatagaccg tgaagccctc
     2401 gttgagccag aaatcgttcc agttgctgtt ggtcaccaag ttgcccgacc aggaatgcgc
     2461 cagttcgtgc gccaccagag agaccaaact gcggtcgcca gtgacaacgg tgggcgtggc
     2521 gaaagtgagg cgcgggtttt ccatgccgcc aaaggggaag cccgtgggca gcacgatgag
     2581 gtcatagcgg ccccatacat aagacccgta gagcgcttcg gcggcctcca ccattttttc
     2641 catttcggca aactcccatg cagatttttc gagcatcggc ggctcggcgt acacgcccgt
     2701 gcgcggcccg acagacttaa aggcaatgtc gcctgcggcc agcgcgatga ggtaggtggc
     2761 gaccggctgc tccatgcgga attggtactt gccgtcgggc gatttctgct gcgggttttc
     2821 ggcgctcatc acggccatca tgcccacagg tgtctgtatt tcagcgttgt aggtgatgcg
     2881 attgccgggc gtatcctggc agggaatcca ggagcgggtg aggatggcct ggccctgtgt
     2941 gaagaggaag ggcagttttt tgccggcggt catggagggt tccatccagc ccagcgcggc
     3001 ggcatcgggg cgggtggcgt aataaatggt gagcacatcg gcgcagggtt ctaattggat
     3061 gcgcagggcc gaaccgaaaa tggaatcgcg ggcgccgagc gagaatttgg cgggtttttc
     3121 gtctttgccc atcgttattt tttggatatc gagattatcg gtatcgaaaa taatttcgga
     3181 ggcgccggca ggattttcga tggtccagcg ggcgtaacca ctcagagttt tggtgtcgaa
     3241 atcaatgagg aggttcaggt ctaaatgttg agcgcgggcc agatcgggac gagagaagga
     3301 gtgcggatcg ggtgcagccg acggcgcaga agcagccggc ttttgctgtt gacaggccgc
     3361 caggagcagc gaaacaaaca ggatagcgag gtggtggaat ttcatgtata ttgttgattt
     3421 tgtggatgtt ttatgactgc cgagagctaa cagctctacg gcggcattca actgagagct
     3481 attagctctg cgggcggctt caaaccaacc gcctcacgcc cgtacccgcc tgcaatttca
     3541 ggtcgctgcc gatgtcgagc aaattgaggc ccggcgtttt gcccgtttcg atgctgccca
     3601 gatcggcctc gaaaccgagt gcgcgggcgc cgttgagcgt agcccagcgc agcagcgttt
     3661 caaaagacac atacgactga aaacgcgcaa tggttttcat ttcctccagc accgagagtt
     3721 gccagttaga cgtgaggctg tcggtgccga tggtcatttg ggcctcggcg tccaaaaaaa
     3781 agcggtagtt cggcaggcgg ttttcgatgt ataagttggc attgggacag gtagcccaga
     3841 acacccggtc gctccagctt tgggcggcct gtatgtcatc ggggctgcac attgtattgt
     3901 gaacgaagag gttgcgttgc tgaggccgca tgttttccat tgcgtaatgg atggagttgc
     3961 gccctgtggc atcaaactcc gtcagcggaa tctggaacat ttcataaaac tcctgaaacc
     4021 cgccggtttt gctgcgaaac agctcgtctt cgtgcggcgt ctcctgattg tgaatgctga
     4081 ccgtagcatc gtcggacttg agtgcgcgga tgcggcgaaa cagttctttc gannnnnnnn
     4141 nnnnnnnnnn nnnnngtgcg gcacggcgct tttgcggttg ccatgcgcgg tgctctggcc
     4201 ttcccacacg ggcagatatt ggttaaaagt ctgttcggcc cggctatctt gcagaaaatc
     4261 aaacatttcc acgaaagtgt aatatctgat tcggctctga tctttgacgg cggcggtgtc
     4321 caatttattg gaaatatcgc ccacggccac aatgcccgca tcgtacatgt cggcatcggc
     4381 gcggcgaatg gcctcctgaa tttcctcctc cggcacattg cgaaactgca ccacgctttt
     4441 caaaaacggc agtagcccgg tgccggtgtc cacccggcct ttcatgtgcg agagttcgag
     4501 gtggcaatgc gtgttgacga agcccggcac gatggccccg cggcgaatgt ccaaagaggc
     4561 cggatcgtgt tgttcgcggc tttctacggc taagattttg ccctgctcat cggtgacgac
     4621 cacgccgttc gggatgggcg gcgcagagac aggaaagagg aggtcggctg aaaatttgtg
     4681 catcgttgag ttattgtagc ggcggctcta ttcgccgaag ccgttgttca atttattatt
     4741 cggcgactga agtcaccggt accgtattcc gtaccgcctg caacgcatac accatcggca
     4801 gcttgccctc catgccgcga atgtggaacc ggcccggctc tgtctctacg gtattggcaa
     4861 agcaattgta cggcgagtag tcgaactcgc ggaaatctgt gatgcggagg ccgctgtgca
     4921 gcagcgccct gaagacctga tcgaggctgt gattccaact cacgcaggtg gtttcaatat
     4981 cggcgtcgcg gtcggtatag gtgccttttt cggtttccac aatggcgtcc tgctggaagt
     5041 aggaatactc gattttggta aaatcattgt cgaacatcca cacggcgggg tgaaattcag
     5101 caaacacaaa caggccgccc ggcttgaggt agcgcgccac gatcttgccc caggcatcga
     5161 ggtcgggcag ccagccgatg gtgccgtaag aagtgaacac cacgtcgaac tgctcgtcca
     5221 agtgttcggg cagatcatag atgttgcagc agatgaagct ggccggaata tcctgttgtt
     5281 cagccaattg ccgggcctga tctatggcgg catccgacaa atcaatgccg gtaacgcggg
     5341 cgccgagccg ggcgagggag agtgtgtcca tgccgaaatg gcattgcaaa tgaagcaggt
     5401 ttttgccttg caggtcgccc agcaagtcca attcgatgct attgagggtg gactgtccgg
     5461 ctaaaaaatc tttaacgccg taaaagtcag attggacatg cacgccggtc cgcttgttcc
     5521 aggagtcgcg attgagttcg atgtagttca ttggtagagt gagtgagtga tgagtgagta
     5581 agtggttagt aggattcgag aaaattgtcc agcaacgcca attcacagaa gtcttcgccg
     5641 gcgaaatgga cgatgagttt tttcatcggt tgtaactatg attttttcgc cgttgatggc
     5701 tgctgccgac tcaaataaat attgatcgct tccgtagttg ccgacctcca gtttttccaa
     5761 caccgatgtc ggcagttctt caatttcttc ttccgataag aatttacagc ggttgctgtc
     5821 cagcaaaatc agcttgatga aggagcgaat gggctgcaca atttcgtcca tacgctgata
     5881 gtctttggca tagcgatata ttttatccaa aaatggagaa ggttctttca caactaagcg
     5941 gtcattgcgc tcaaggaaac ggttgagaaa tgtttccaaa gacttttttt cctctgtact
     6001 cgcttcggac aggaaatact cgggcagcca ctcgttcact accaagtcca tatcagtccg
     6061 tcaatttgaa gaaagatttc actgatttga gttcttctgc aaaaaaatga gaaagccctc
     6121 cttccaactg acctttcgag ttgatggact ggtgttcgac agccgtgccg gtctcagtca
     6181 tagacaagtg atataccttc acatcttcgg ggcgcaggct gccggcagca acttttgcca
     6241 tcagggcctg cacaaaatgc tcgctgtgag tagatactaa aaattggcgt ttggggtcac
     6301 tcgacaccat atcggcaaat gaagccgcta aactgcgcac catgccggga tgcaggtgga
     6361 tttcgggttc gtctatacaa ataaactgct tgcccggctg gaatatcttg gccaacaaag
     6421 tgacaacctg attggtgccg gaaccttcgt ttaccaaaaa attggtctgc cccgactgcg
     6481 catttctggc caacagactg aacaaaccag gggtgccgtt gcccggcaat tcaagcgttc
     6541 tgcccgttat tttttccaaa tactgattga ctgcctccaa cgtagccggc tccgatttca
     6601 gcatggtggc tacttcttcc tccgtttgga tgatgccccg catgggcacc atgttgtaat
     6661 atggtttggt gaaatttcga gaaatgggag cgaaatcggt ggcaaatagt tcgttcacag
     6721 gagtttgcaa cgctgaaatg atactttctt caagttctga aaaaacatca agccgtggtt
     6781 tcaatgatgc agtgaatcca ttccatccga acaatatttc agtatcattt cttttaaatt
     6841 gcgctttttg atatgtttct gtcacaggaa cgtaaggcaa tcctatgctc atacgcgctt
     6901 caaaactgaa cggccctgaa tcggacgata atttaatgaa agtcttaccc gcccccagaa
     6961 aaaggcgata tgaaacgcca aaagcgccta aattgctttc ttgctctgcg gtgatcccta
     7021 aatcaatata ctcattgagc gcatcaaact tcgacttcac ctccgaaaac ccgcccagat
     7081 tgataaaagg cagcgcaaag aggtcttcca gactgcggtt ggggttgcgc aaaaaatcct
     7141 gcaggatcat caaaccatac agaatactgg acttcccgct gttgttggcg cccgtcagta
     7201 cggttatttt accaagctct atcggactga tttgcccgat ggaccggaag ttttgaatat
     7261 gtatttttga aatcatctcg acagcaattg gccataaaag tacagttttt tgtggtatga
     7321 gagtttcaca gcgccaaaaa ctcccgaaat gcagccacta caacttacga taaaaatatt
     7381 tatgttacaa atcttgactt ttatgaaaaa tatcgtaacc ttgcacagct tttagtaaca
     7441 tgagcctatg gccgaagata ccaccgacag tccgaaatac gcagaaatcc tcgaagctgc
     7501 ccgccgcctc tactggaaat acggcatcaa aaaggtgagc gtggaggaaa tctgccgcga
     7561 agcgcaagtg agcaaaatga ctttttatcg cttctttccc aacaaaattg aagtcgccaa
     7621 agtcgttttg gagcaagttt tcgacacctc cctgcgcgac taccgccaga tcatggcaca
     7681 agacgtacct tacgaagaga aggtccggca gcagcttcgc ctcaaatttg aaggcaccga
     7741 agccatcagc gccgacctca tccgcgactt gtactcgaac gaagaatggg gcctcacagc
     7801 ctacctcgaa caacgcaccg aagagacgat ccgggagatc gttgccgact tcgcgcacgc
     7861 acagcaacag ggctgggtcc gagccgacat ccaacctgcc ttcctgctct acctcttcca
     7921 ccaaatgccc gcctgggtga cgaacgacca attgctggct tcgtacccgc accctcgcga
     7981 tctggtgatg gaagtgatca attttttctt ctacggcatc ctcgaaaagt agtgcattca
     8041 agatggtaca ggctacaagt ctcaagccat aagcttcagg cttgatcttc catcagtaaa
     8101 tgagtcttaa atttcggaaa aatctccatg cgctctttcg aagaaaattc tacataatga
     8161 tcagataacc aaaacttgca gcttgaggct tacagcttgc agctaactaa ttaccatttc
     8221 cgctcactcc tccaaaatgt tccgcatcct catcatcggc ctttttctcc tctcctccac
     8281 gcactgcctc gcacaggaca gcaccgcatt gctgcgcgac agccttgttt ttaaaggtca
     8341 actcatcaca tggctcaatg ccaatctcgc caacgagcta cccctcgccg ccggactgcg
     8401 ctatctgccc caactcaact accgcctgcc gcacactgtc accgccaacc gcacaccgcc
     8461 aaccgctcac cgccaaccgc acactgcccc ccgcctcttc gacctcgaag cctccgccaa
     8521 catcttcgcc gctgccaatc tccgtccttt cgactcgctg aacgccacgg gtctcatcaa
     8581 gccctatcgc ctttgggtgc gctactccgg gccgcaatgg gaaatccgcg ccggactcca
     8641 aaaaatcaac ttcggctcgg ccacgctgct ccgcccgctc atgtggttcg atcagattga
     8701 cccccgcgac cctatccagt tcaccgacgg tgtgtggggg ctgctgggcc gctattattt
     8761 tctcaacaac gccaacctct ggctctgggg tttgtatggc aatcaccaac gccggggctg
     8821 ggacctcgcg cccaccgcaa agtaccgacc ggaatggggc gccagatttc aacaaccgct
     8881 gcccaaaggc gaaatggccc tctccctgca tcaccgccat gccgacacgc gcagcctcga
     8941 accgctttta cctgcccacg ccgccgctcc cgaaacccgc ctcggtttcg atgtaaaaat
     9001 ggactgggcc gtgggttgct ggctcgaagc ctcctggacg gccaaccacc gcaacctcgg
     9061 cgcctaccgc catcaggaaa tgctcaatct gggcatagac tacacctggg gcatcggcaa
     9121 cgggctgtac atcgccgccg aacaactgct cgtcgccacc gatacgcgag cattccgact
     9181 ggcgcgcacc aactcgctca gcctgctctc attgtcgtac ccgatcggct ttttcgacaa
     9241 cctcggcgct atgatctatt tcgactggag caacaacgca ctctatcgct tcgtcaactg
     9301 gcgaaaacaa
//
LOCUS       DAORLF010000151         9296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4002_length_9296_cov_2.380803, whole genome shotgun sequence.
ACCESSION   DAORLF010000151 DAORLF010000000
VERSION     DAORLF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_9296_cov_2.380803"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PK198_12875"
     CDS             complement(<1..766)
                     /locus_tag="PK198_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39675.1"
                     /translation="MGHEDGAHSDERPGQYRAIATNEWGCANESAPQTVLAGPDIARI
                     PSGCHSRCNPDTLCFPAVPGVNAYQWFFNGAAQGSASGSVPQIVITQSGAYYMRMTTT
                     DGCVLDSEPLTVDLFDGYGSLRGIVYWDRNDNGSYDPADSLLTGVPLILLSGNTPLDT
                     VLSQTGGQYGFANILSTGYGLQLDTAALPFGLVPDTVRIDTALVGCDVEVLVNWRVRC
                     VNTTEEVTLQACPGRTVSYNGNTLTAGEVRSFTLVNA"
     gene            complement(709..2517)
                     /locus_tag="PK198_12880"
     CDS             complement(709..2517)
                     /locus_tag="PK198_12880"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF39676.1"
                     /translation="MSELPAPYVSLTTPDNAGLCPGDPPSTLYATESEAGFSYVWLRD
                     GSPIAGANASTFSTDVLGVYQVEATNANGCRALSNPLTLFFYCAPGGGGGFPGGGFPG
                     GGGGNPSNCDPNTLIQFNIQATANCNVSNFTNTTPAFIPGTLSWNFGDPGSGADNVST
                     LENPTHTFSRAGFFRVALFGQVLNPTPENCYTIRVDTVPVAAGFYADIACAGAPAAFT
                     DASTFLPGESIASRAWDFGDPASGADNNSAATHPTHVFATPGIYTITLTVTAANGCTA
                     VISKTIEVVAPPTAAFPDPSVLCAATATPFIANVSADAIEFGWNFGDPASGDANASSL
                     EQPFHRYENPGSYSVRLEATNIYGCTADTLRSITILPNGLSGSITFTSPICTGDSTLL
                     TAPSGGVRWQWSTGDTTSTVWVRDAGIYTLLLTNADGCEYQPAAAVVNVLPPPVTAIR
                     GVRYNALGLPESYTYDSLSVCVGEDVFLEVIAQTGQTYQWSTGQPGTNIEFSENRGNQ
                     LPVGQQDIFVTIRETATNCTNVIGPFRIWVRPLPNVPLVSASPAGVVCEGTPVSFNIT
                     NSQAGVNYSWSSGANGNTWVMKTAPIAMNAPANTAP"
     gene            complement(3663..5402)
                     /locus_tag="PK198_12885"
     CDS             complement(3663..5402)
                     /locus_tag="PK198_12885"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF39677.1"
                     /translation="MNQFSQKRLWACLVFTLMLGTAQAQVSNITFFIQGPEVVFPGTC
                     DTFRVVSSMPNPGGSNVVVTNVTWTVSNGLTAQGNPALFCFDIPGATYLLFATGLASV
                     NGAPFTFQTQPYTVQVLETCGFNIPFQITSNASSLCAANLPPQPVSPPPTGFALLLSD
                     INGAVPGQQICMDIQAQNFFNMSGMSFSMQYDPARLLFASMTNVGLPGSTLFGLPSPP
                     NSGTTDPGAITVSWSSPDQSITLNNGTVLFRLCFFVLPGSGCTTDVVFTNEPAPIRIL
                     RGSGEIVSFNSRSGRVQISNSSMGSDPNSASCCEKVCEGAVVQYNVDIPTGAIHPVTW
                     SVQGAQSYTVNNFGYGVEVVWGTAGSGSVTAAVYDNTAQICVDILAIPEAGFTTNPPT
                     VNDTVRLCRGQSLAFTNTTVGAESHRWVFGDGTSSTELNPVHQFEDAGLYRVALIAYN
                     ACLCSDTAFVMVQVDPAIAPQVTCRGTVCEGTAVTYTTDADCGTFIWTVSANGTITNG
                     GGPADHFITIDWAGGQEGIITLQVSNCNNATYCPAPNELHVPVISESCCRKASPNQTT
                     RNSRMRSFTSSNP"
     gene            5612..6040
                     /locus_tag="PK198_12890"
     CDS             5612..6040
                     /locus_tag="PK198_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39678.1"
                     /translation="MKKFLSIAVFALFGLVGYAQTTQLTVYYEFRNIEDGYDHETKTQ
                     VVIDGKSVGESMPHKQSKKGILTVNVPTGAHKLRVMNWAKYEGTWEEHTVTNNYSIDC
                     MHEESHSFSKPEKLYLVFDLNSSGTIASWKKPVKMKKGKK"
     gene            6154..6735
                     /locus_tag="PK198_12895"
     CDS             6154..6735
                     /locus_tag="PK198_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HRF39679.1"
                     /translation="MPTQFLPAAIQQFEYYKLIGERAMAQLPDEALLFQPTPESNSIA
                     VIVKHLWGNMLSRWTDFLTTDGEKEWRQRDAEFESEHASRDEILARWNEGWACLFTAL
                     NGLSEADLGAVVYIRNEGHTVLEAISRQLAHYPYHIGQIVFIGKMVQDSDWTSLSIPK
                     RASDAYNAGKFAQPQSIRHFTEEWKETASTSKE"
     gene            7070..7765
                     /gene="trmB"
                     /locus_tag="PK198_12900"
     CDS             7070..7765
                     /gene="trmB"
                     /locus_tag="PK198_12900"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRF39680.1"
                     /translation="MSKRNKLMKFAEMAAFPNVYENFDVGRPVLHTAGKEVEMAGRWA
                     EGHFGNNNPITLELACGRGEYTLGLARMYPDRNFIGVDIKGARIWKGAHIALEEGLPN
                     AAFLRTRIELLEQFFQAGEIAEIWITFPDPFLRNSKSNRRLTSPKFLETYQRLLPSGG
                     KVHLKTDSPELYEFSLETLEAVPEIQILAQNSDIYAGDLPLPELALKTYYENMHLADG
                     KTIKYLCFRWAGE"
     assembly_gap    7841..7885
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7886..8348
                     /locus_tag="PK198_12905"
     CDS             <7886..8348
                     /locus_tag="PK198_12905"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF39681.1"
                     /translation="IEEMLEPVPFSSARSIVEMGAGDGRITDAIIRHMSPESQLLVFE
                     LNDVFFAGLARIQDARVQCLNQNAADLLQWVQPDSVDAVISALPLAFIPKAEKNRILR
                     AAAESLKPGGLFVQFQYSLTDYGLLRRHFGKVGVRFELLNIPPAFIYVCTK"
     gene            8384..>9296
                     /locus_tag="PK198_12910"
     CDS             8384..>9296
                     /locus_tag="PK198_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39682.1"
                     /translation="MIRWKHWEYWPSWAYYFPMLAYYPWLALKARHLCFFTAANPGIY
                     TGGMGMESKYETLMKIPAEWRPKTILALPGESTESLSLRLEQAGIGFPLIAKPDMGLR
                     GFLVSKIYDLAALQTLLEKHPVPFVLQEFISKKNEFGVFYHRFPDEEQGHITSLTFKE
                     FLHVVGNGQDTLRQLIAAHPRALLQWERLSKSHAEKMEIIPPAGEIVPLGEIGNHSKG
                     TRFINAAAHIDDTLRESFDRMVDEVAAMIEPFAHRAAIQGQIEHLHMLGTSGTVTTLA
                     GVHLGLPKYDRRRVDGAWLGANEVDVIL"
BASE COUNT         2189 a         2556 c         2603 g         1903 t
ORIGIN      
        1 ccgcattgac caacgtgaaa ctgcgcacct cacctgccgt cagcgtattt ccattataag
       61 acaccgttct gccgggacaa gcctggagcg tcacttcctc tgttgtgttc acgcagcgta
      121 cacgccaatt gaccaacacc tccacatcgc agcccaccaa tgccgtatca atgcgcaccg
      181 tgtccggcac gagaccgaaa ggcaaggcgg cggtatctaa ttgcagaccg tagccggtgg
      241 agaggatatt ggcaaacccg tattgcccgc cggtttgcga cagcacagta tccaacggcg
      301 tattgcccga caacaagatg agcggcacgc ccgtgagcag cgaatcggcc gggtcgtagc
      361 tcccgttgtc gtttctatcc caataaacaa tgccccgcaa actgccgtag ccgtcgaaca
      421 ggtccaccgt cagaggttcg gaatccaaaa cacagccgtc tgtggtggtc atgcgcatgt
      481 agtaggcacc gctttgggta atgacaatct gcggcacgct gcccgatgca gacccttgcg
      541 ccgcgccgtt gaaaaaccat tggtaagcat tgacgcccgg cacagcgggg aagcacaaag
      601 tatcgggatt gcagcggctg tggcaaccgg agggaatgcg tgcaatatcg gggccggcca
      661 gcacggtttg cggcgcgctt tcattggcgc agccccattc gttggttgct atggcgcggt
      721 attggccggg gcgttcatcg ctatgggcgc cgtcttcatg acccatgtat tgccattggc
      781 acccgacgac caggagtaat tcacgccggc ttgcgagttg gttatattaa aactcacggg
      841 cgtaccttcg cacaccacgc cggcgggtga ggcgctcacc aaaggcacat tgggcagggg
      901 gcgcacccaa attcggaacg gcccgatgac attggtacaa ttggtggctg tttcgcggat
      961 cgtcacaaaa atatcttgct gacccacagg cagttggttg cctctgtttt cagaaaattc
     1021 gatgttcgtg ccgggctgcc cggtgctcca ttgataagtc tgtccggttt gggcaatgac
     1081 ttccaaaaac acatcctcgc cgacgcagac cgaaagtgaa tcgtaggtat aactttccgg
     1141 caggcccagc gcattgtagc gcacgccgcg tatggccgtc actggcggag gcagcacgtt
     1201 cactacggca gcggcgggtt ggtactcgca tccgtcggca ttggtcagca gcagggtgta
     1261 aatgcccgcg tcgcgtaccc ataccgtaga ggtggtgtcg ccggtactcc attgccaacg
     1321 cacaccaccg gaaggcgccg tgagcaaggt gctgtcgccg gtgcagatgg gagaagtgaa
     1381 ggtgatgctg ccgctcagtc cattggggag gatggtaatg ctgcgcaagg tgtcggcggt
     1441 gcagccgtaa atattggtcg cttcgagtcg gacggagtaa ctgccggggt tttcgtagcg
     1501 atggaaaggc tgctccaaag aggaggcatt agcgtcgccg gaagcgggat cgccgaaatt
     1561 ccagccaaat tctattgcat cggcactcac attggcgatg aacggcgtgg cggtagcggc
     1621 gcacaaaacg cttggatcgg gaaaagctgc cgtcggcgga gctaccacct ctatggtttt
     1681 ggaaatgacc gccgtgcaac cattggctgc cgtcacggtg agggtgatgg tgtaaatgcc
     1741 gggcgtggca aaaacgtggg taggatgcgt ggccgcgctg ttgttgtcgg ccccggaagc
     1801 gggatcgcca aagtcccagg cccgcgatgc gatgctctct ccgggcagaa acgtggaggc
     1861 gtcggtaaag gcagccggcg cgccggcaca ggctatatct gcataaaaac cggcagctac
     1921 gggcaccgtg tccacgcgaa tggtgtaaca gttttcaggc gtcggattca aaacctgtcc
     1981 gaacaaagcc accctgaaaa atcctgcccg actgaaggtg tgcgtcggat tttccagagt
     2041 agagacgtta tcggccccgg aacccggatc gccgaaattc caactcaaag tgccgggaat
     2101 gaaggccggc gtggtgttgg taaaattgga aacgttgcag ttggccgtag cctgtatatt
     2161 gaattgaatc agcgtgttgg gatcgcaatt ggagggattg ccgccaccgc cgcccggaaa
     2221 tccgccgccg gggaatccac caccgccgcc gggagcgcag tagaaaaaga gcgtgagcgg
     2281 attggacaat gcccggcaac cgttcgcatt cgtggcctcc acctgataca cgcccagcac
     2341 atcggtggaa aaagtggagg cattggcgcc tgctatcggg ctgccgtcgc ggagccagac
     2401 gtacgaaaaa ccggcctccg attcggtggc gtacaaagtg gaaggcggat cgccggggca
     2461 cagacctgca ttgtcgggcg tggtgagcga aacatacggc gcgggcagtt ccgacatgtt
     2521 gaatcgggcc gagccggcgc agccgaaatt gtcggtcacg atgagttggt aataccccgc
     2581 cgggagattg gctgcggcca cattggaaac caactgatct gtcgcgttgc gccactcgta
     2641 agcagcataa gcgccgccca cgctcaccat cgccgttgtg ccctcgcaca ccgatgcagg
     2701 cgctgcgacc atcggaaccg ggcgggcgtg taccgtcacg gaccgggaaa tgttttggcc
     2761 gcacacgttg agttgcagcg ttgccgcacc gggccgatgc cacagaatct caaccgaggc
     2821 actgttgccc acctgataaa tgctgcccgc gccggccggt accacagacc attggtaatc
     2881 caattgcgga aaggacgtag ctgtgtaagt agcacgctca tcctcgcagg catctgtcgg
     2941 gccggaaaca gcaatggccg gcaacacgga agcgccaaaa cccagcggag gcgaaacgca
     3001 accctgcgga cttacctgcc tgaccgacaa ggtataagac ccggcagccg cccattgcac
     3061 cgtcacggga ttgccgctga gagtagccgg tccgacattg ccggcaaggc tccaattcag
     3121 cgtatgagcc ggcaatgcgc cgccaccgct gtattggtaa gcctggccgg ggcaaatcac
     3181 attttctccg ttaataatgg ccaatgcagg cggcaaatcg agtaccctga ccgttatctc
     3241 atacaagggg atacaaaaat ccgtcggaac cgccggccgg gcgatcaaac gatacgaacc
     3301 cagcccgaaa ttccaggtga gcgacggcgc agcagtagcg gccacggaag tccagaccac
     3361 agagccgtcg ccggctactg cctcccagtt acagggtacg ttggcgccgt tgtcggtgcg
     3421 tcgggcggta taattttggg tactgttcag gcaggcttcc accgggccgg agacagcaaa
     3481 ttcggggcgt atgttcacca tgatggtatc ccggccgccg cattccaaat agcaattgta
     3541 atattccacg gccacccact gcggctgtgt gctcagcaag cccgaccatt gcactactac
     3601 ttcgtgcgtg ccctgcccac tttggatatt gcccatgccc gcgaagtact gaacaaagac
     3661 cactatgggt tggaggatgt gaaagaccgc atcctcgagt tcctcgtcgt ctggttcggt
     3721 gacgccttgc ggcagcagct ttcgctgatg accggtacat gcagttcgtt gggcgccggg
     3781 cagtacgttg cattgttgca attggaaacc tgcaaggtga tgatgccttc ctggccgccc
     3841 gcccaatcaa tggtgatgaa atgatccgcg gggccgccgc cgttggtgat ggttccgttg
     3901 gcgctgaccg tccagatgaa agtgccgcaa tcggcgtcgg tggtgtaggt cacggctgtg
     3961 ccttcgcaaa cagtaccccg gcaggtgact tgcggcgcga tggcggggtc cacctgcacc
     4021 atcacaaagg ccgtatcgct gcaaaggcag gcattgtagg caatgagcgc cacgcggtac
     4081 agcccggcgt cttcaaactg atgaacgggg ttcaactcag tactcgaagt gccgtcgccg
     4141 aacacccagc gatggctttc cgctccgacg gtcgtgttgg taaacgccag agattgcccc
     4201 cggcacagac gaaccgtgtc gttcaccgtg ggcggattgg tggtaaaacc ggcctccgga
     4261 atggccagaa tgtcaacgca aatttgagca gtattgtcat aaacggctgc agtgaccgac
     4321 cccgatcccg cggtgcccca cactacctcc acgccgtagc cgaaattgtt gacggtgtag
     4381 ctttgagcgc cctgcacgga ccaggttaca ggatggatgg cgcccgtagg tatgtccaca
     4441 ttgtattgaa ctacagcgcc ttcgcaaact ttttcgcagc atgatgctga attgggatcg
     4501 ctccccattg aactattaga gatttgtacc cggcccgaac ggctattgaa agacacaatt
     4561 tcaccgctgc ctcttaatat tctgatgggc gccggctcat tcgtaaacac cacatcggtg
     4621 gtacagccac tgccgggcag cacaaaaaaa caaaggcgaa acagaacagt gccgttgttg
     4681 agtgtgatgg actgatcggg cgacgaccag cttacggtga tggcgccggg gtcggtagtt
     4741 cctgaattgg gcgggctggg cagcccgaac agagtgcttc ccggcagccc gacattggtc
     4801 atggaagcaa acagcaagcg ggcagggtcg tactgcatac tgaaagacat gccggacata
     4861 ttgaaaaaat tctgcgcctg aatgtccatg caaatttgtt ggccgggtac cgctccgtta
     4921 atgtcggaga ggagcaaggc aaaacctgtg ggcggcggcg ataccggctg cggaggcagg
     4981 ttggcggcgc acagagagga ggcgttggag gtaatttgga aagggatgtt gaatccgcag
     5041 gtttccagaa cttgcacagt atatggctgg gtttgaaatg taaagggcgc cccattgacg
     5101 ctggccaggc ccgtcgcgaa aaggagataa gtcgcaccag gaatatcgaa acaaaacaaa
     5161 gctggattgc cctgtgccgt cagcccattt gaaacagtcc aggtcacatt ggtaacgact
     5221 acattgctac cccctggatt gggcatggaa ctcaccaccc tgaaggtgtc gcaggtgccg
     5281 gggaatacaa cttccggtcc ttgaatgaaa aatgtaatat tgctgacctg ggcttgggcg
     5341 gttcccaaca taagtgtaaa gacgaggcag gcccacaggc gtttttggga gaactgattc
     5401 atggtgattg tattttgttt aagagatact cagaatgtgg ttgtccgctg cctgtcgggt
     5461 ggaaataatt cttatgttat gtggtaagct gttggccgca agcccttagc tgcaagccac
     5521 cagccgatcg tagttcgcag cttgaggctt gcagctaaat ctaaaaaatt acctttgcgc
     5581 cattactcac attttaatct caaatactac catgaaaaaa tttttgagca ttgccgtatt
     5641 tgcgttgttc ggcttagtcg gatacgccca aaccacccaa ctgacggtgt attacgagtt
     5701 ccgcaacatc gaagacgggt atgaccacga aaccaaaacg caggtcgtca ttgatggaaa
     5761 atcggttgga gaatccatgc cgcacaagca gagcaaaaaa ggtatcctga ccgtaaatgt
     5821 gcctacaggt gcgcacaaac tccgcgtgat gaactgggcc aagtacgaag gcacctggga
     5881 agagcatacc gtgaccaaca actacagcat tgactgtatg cacgaagaga gccattcctt
     5941 cagcaagccg gaaaaactgt atttggtgtt cgatctgaac agcagcggca ccatcgcttc
     6001 gtggaagaag ccggtgaaaa tgaagaaggg gaagaagtaa aaagccgaga gcaaagtgca
     6061 tcgtgtgctt agctattctg caagaatcct gatttcgacg cctgcataat ccgtgggcgt
     6121 cgaaatctgt ttcataaatc aacgatccga ccaatgccca cccaattcct tcccgccgcc
     6181 atccaacaat tcgagtacta caagctcatc ggcgaaaggg ctatggccca attgcccgac
     6241 gaggcactgc tgttccagcc cacgccggag agcaacagca ttgctgtgat tgtcaaacac
     6301 ttgtggggca atatgttgtc acgttggacg gacttcctca ccaccgatgg cgaaaaagaa
     6361 tggcggcaac gcgatgccga atttgaatcg gagcatgcct cccgcgatga aattctggcc
     6421 cgctggaacg aaggctgggc ctgcctgttc accgctctga acggcttgtc ggaggccgat
     6481 ttgggcgccg tcgtttacat ccgcaacgaa ggccataccg tactggaggc catcagccgg
     6541 caattggcgc actatcccta ccacatcggt cagatcgttt ttattggaaa aatggtgcag
     6601 gattccgatt ggacctcgct ctccattccc aagcgcgcct cggatgcgta caatgccggg
     6661 aaatttgcac agccccagag catccggcat tttacagaag agtggaagga aactgcaagc
     6721 acatccaaag aataaagtga ggcgcagttt tcggacagtg tccaatttga caaatcttcg
     6781 agatttgtca aattttcccc gcccggttct acctttagcg gactatacag aagagtggaa
     6841 ggaaaccgcc caaccgaaaa atatcaagtg ataaatgtaa aacccgctcg cgacgccctc
     6901 tccgagcagc gaaaatcata aaaaccataa aaaattagcg tcaaaaaaca agccgactct
     6961 attggcctga catgcgaaaa tcacaggcat ccaatccaaa atctactttt tccctaactt
     7021 gcacccctga aaaatggcgg ctactcgact gccgcatcag cacactctta tgagcaaaag
     7081 aaacaaattg atgaagtttg ccgaaatggc agcgtttccc aacgtatatg aaaatttcga
     7141 cgtggggcgg cccgtactgc acaccgccgg caaagaagtg gaaatggccg gccgctgggc
     7201 cgaagggcat tttggaaaca acaatcccat cacattggaa ttggcctgcg ggcgcggcga
     7261 atacacgctg ggactggcgc gcatgtatcc cgacagaaac ttcatcggcg tggacatcaa
     7321 aggcgcgcgc atctggaaag gcgcacacat cgcgctggag gaggggctgc ccaatgccgc
     7381 cttcctgcgc acccgcatcg aactgttgga acagtttttc caagccggcg aaattgctga
     7441 aatctggatc accttccccg atccgttttt gcgcaacagc aagtccaacc gacgcctcac
     7501 gagtccgaaa tttttagaaa cataccaacg cctcttgcct tcaggcggca aagtgcatct
     7561 caaaaccgac tcgccggagt tgtacgagtt ttcgctcgaa accctggagg cggtgccgga
     7621 aatacagatt ttggcacaaa acagcgatat ttacgccggc gatctgccgc tgcccgaact
     7681 ggcgctgaaa acgtactacg aaaacatgca cttagcagat gggaaaacca tcaaatatct
     7741 ctgttttcgt tgggccgggg agtaaagttg cctttaaatc accaatcatc taaaacatgg
     7801 ctgccaaatc tggtttttta aaaactgcta tcaaagactt nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnncattg aagaaatgct ggaacccgtg ccattcagct
     7921 cggcccgcag catcgtggaa atgggcgccg gcgacggccg catcaccgat gccatcatcc
     7981 ggcacatgtc gccggagagc caattattgg tatttgaact gaacgatgtt tttttcgccg
     8041 gtttggcccg tattcaggat gcccgtgtgc aatgcctgaa ccaaaatgcc gccgatcttt
     8101 tgcaatgggt gcagcccgat tcggtggacg ccgtcatctc tgcgctgccg ctggccttta
     8161 ttcccaaagc agaaaagaat cgcatcctcc gcgccgctgc cgaatcgttg aaacccggcg
     8221 gcttgtttgt acagtttcag tattccctca cagattatgg tttgctgcgc cggcatttcg
     8281 gcaaagtggg cgttcggttt gaattgctca acatccctcc ggctttcatt tatgtgtgta
     8341 ccaagtgatg cctacagatg cgcatttccg gcaacgcggc cgcatgatcc gttggaaaca
     8401 ctgggagtac tggccctctt gggcgtacta ttttcccatg ttggcgtatt acccctggct
     8461 ggcgctcaag gcccggcatc tctgcttttt cacggcggcc aatcccggca tttacaccgg
     8521 cggcatgggc atggaatcga aatacgaaac cctgatgaaa atacctgcgg aatggcggcc
     8581 caaaacgatc ttagccctgc ccggcgaatc tactgagtct ttgtctttgc ggctggaaca
     8641 ggccggcatc gggtttccgc tcattgccaa gccggatatg ggattgcggg ggtttttagt
     8701 ttccaaaatt tatgacctgg cagctttgca aacgctgctg gaaaagcacc cggttccttt
     8761 tgtattgcag gaatttattt ccaaaaaaaa tgaattcggc gtgttctatc accgcttccc
     8821 cgacgaagag caggggcaca ttacttccct cacattcaag gagttcctgc acgttgtcgg
     8881 caacgggcaa gatacgcttc ggcaattgat agcggcgcat ccgagggctt tgctgcaatg
     8941 ggaacgcctc tcgaaatcgc acgccgaaaa aatggaaatc attcccccgg caggcgaaat
     9001 cgtgccgctc ggcgaaatcg gcaatcactc caaaggcaca cgtttcatca atgctgccgc
     9061 gcacattgac gatactttgc gcgagagttt cgaccgcatg gtcgatgagg tggccgccat
     9121 gatcgagccc ttcgcccatc gcgcggcgat ccagggccag atcgagcacc tgcacatgct
     9181 cggcacgtcc ggcacggtaa cgacgctcgc gggcgtccat ctcggcctgc cgaaatacga
     9241 ccgccgccgc gtcgacggcg cctggctcgg cgcgaacgag gtggacgtga tcctcg
//
LOCUS       DAORLF010000152         9294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4004_length_9294_cov_1.928023, whole genome shotgun sequence.
ACCESSION   DAORLF010000152 DAORLF010000000
VERSION     DAORLF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_9294_cov_1.928023"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>272
                     /locus_tag="PK198_12915"
     CDS             <1..>272
                     /locus_tag="PK198_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39683.1"
                     /translation="SAMVWLPCNSATIARQYRLLLDRYAAETSTQPDFVDWQAHDFSM
                     RGMGGIEGALLSGAAHLLSFTGTDTIPAIDLLEQYYGADAERELIG"
     assembly_gap    273..450
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <451..1045
                     /locus_tag="PK198_12920"
     CDS             <451..1045
                     /locus_tag="PK198_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39684.1"
                     /translation="EDIAGRNGKVVIRPDSGDPVLILCGNPDGATEPERKGVVELLWD
                     TFGGQINSKGYKELASCIGTIYGDSITLERAEQICERLKIKGFASTNVVLGIGSFTYQ
                     YNTRDTFGFAMKATYGEVNGEGRAIFKDPVTDDGTKKSAKGLMQLRKENGRYHLLDEV
                     SREEEKEGELKEVFRDGKLLTEFSLAEIRKNIRHQGV"
     gene            complement(1196..>2834)
                     /locus_tag="PK198_12925"
     CDS             complement(1196..>2834)
                     /locus_tag="PK198_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39685.1"
                     /translation="KDDAAYTTPGVPVEFNVLENDAFGTAATMFTIVTSPANGTLVLG
                     PENGLVTYTPNPGYYGTDSFKYSSRRPFGASNLETATAKVFVSNFAPSAATYRMVTPK
                     ATPLVIGNNVPVPNFQFFVTDQPDNGSVLLLQGEVDTVILGRSIKGYNMVVYVPHATV
                     NAGEDEFEIAYCIPNSNGSAGCSFMRTVKIEIDILDIGAGNEPMCFGDCIWAGDANFD
                     GVVNMEDLLPIGLFMGEAVLPRPDNSPALWYGKHSENWDNPFVNYNVDLKHLDADGNG
                     YVSALDTAAIHNFYGRTHSITVSKAPLTDQTIHLSGNIFAGPGDVVELKMILGTSAKP
                     AVNVYGFTFPFLYNPNIFSPASVQTSFSHRGWLAYNSPILFMNHNNMEGRLESGFTRT
                     TARAASGHGEIGTVRFIIRDDIAGIRLSEEELSVEVGGGVSTVMGSDGQTYGVEIEKL
                     NIKISLQKPEAPLTEDLLKAWPNPTSDFLNIHLNGGKEFERIVLYNLMGQEVYNSGSM
                     KARNAQIGTSQLANGIYVLSVVTPEGRLNKKIEVIKE"
     assembly_gap    2835..2951
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2952..3688)
                     /locus_tag="PK198_12930"
     CDS             complement(<2952..3688)
                     /locus_tag="PK198_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF39686.1"
                     /translation="MNVFLQRILYQLLILSVPLILQGQTSILLETVENTPAEYVFNSS
                     PNAPGVVVQAQHGTAVLAPSGTLNGYRLTYTPQLNFIGQDFVRVSRWTCPIPTCFQYL
                     DFYITVVPARVDARQDNVSTSVNTPVTFNVLSNDFSSRGILILNSISSVNNGTATFVP
                     GTPNIQFTPAPGFEGVALLNYIVCDDENNCDNGNVSVHVVNGENTTASDTVLVFTRKA
                     TPQVILIPPGFQRISGPSSGVYSDSGP"
     gene            3775..>5195
                     /locus_tag="PK198_12935"
     CDS             3775..>5195
                     /locus_tag="PK198_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39687.1"
                     /translation="MRNTKLYSILEHFDKYEQNRLRKYLQSPYFNRNQTLIDLFDLLV
                     EDANSEKKKEIEKEDVWTLLRLKSPYDDVRFRKYYSDLLKLVEGFLAQQVYEADPVRQ
                     ATDLLKAVGKKKMEKLYNSSVKTARHLSQNQPWRHAQHYLQQYQIEVNFYDIMAFETK
                     RGARTNLEEIANNLDSFYLAEKLRIYASVLTQQTFVSQEYKVRLIDEIMAYLHAHKDH
                     EETPAVAVYFQVLRTVTDQDNLEHYYRLKELLDQYGLLFPKHEAMELYGSALNYCIRQ
                     INHGNQGFLEELFILYNQLIDKEIIFLNDEFSPWDFRNIVVIALRLGRYEWVEKFIHD
                     FNHKIPELNRDNTVTYTLAQLYFYQKKYDKVIEQLRNVEYDDVSYNLNSKTMLLMTYF
                     ETDEYEPLYSLFESFRTYLNRQKELSSDRKALYKNLIKFTKKLTKIMPGDKAEVKRLK
                     EEIAATGNVASLNWIQEKIEELV"
     assembly_gap    5196..5349
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5350..>5608
                     /locus_tag="PK198_12940"
     CDS             <5350..>5608
                     /locus_tag="PK198_12940"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF39688.1"
                     /translation="QMAIAQPVIQLQTFATGFTRPVDIASAGDERLFIAEQRGIIHIL
                     NAQGQRLPTAFLNIQSRVNSASNERGLLGLVFHPNYAQNGYF"
     assembly_gap    5609..5740
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5741..7419
                     /locus_tag="PK198_12945"
     CDS             <5741..7419
                     /locus_tag="PK198_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF39689.1"
                     /translation="PYSNHNAGDLNFGPDGYLYFGLGDGGSGGDPQNYSQNRQSYLGK
                     MMRIDVNSGSPYAIPATNPFVNDPSTLNEIWAIGLRNPWKFSFDRLTGDMWIGDVGQN
                     AWEEIDFQPAASIGGENYGWRCYEGNATYNTAGCQPATAYKFPIHVYVNGLNSGCSVT
                     GGFVYRGSEFPDLYGHYLYTDYCGGKIWSLTPDGNGGWTNRQLLQGAANQYVSFGENQ
                     NGELFLAAIGVGTILRIREICSTFQVAGTATDETCAGTANGSIQLNITNPGATQTIAW
                     SNGANTGTISNLAAGTYTVTVTSSNTCARTLTYTLTNGSPAAPNLSLAPVEICTGASA
                     SLEAPAAPAGLGYQWYRNGQPVPGATQRILLVTEAGSYTVAFTVTVGCASAQSNPVVV
                     TVSVFPTLPPITAEGNVLTAPDLDVFILYQWLLNGQPIANANGLIYTALESGEYSVQA
                     TTLNGCTYTWPAVTVVVSSTAEIRGLQRFVVSPNPVRDMLAIELQLESSAPMTLRLTN
                     AAGKVVWEQRERIGGLWRTELDMSRLPAGMYSLTLSRSRREQAVVQLVKQ"
     gene            7586..7804
                     /locus_tag="PK198_12950"
     CDS             7586..7804
                     /locus_tag="PK198_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39690.1"
                     /translation="MTDINAVKSRIIQRIQETDEYWVIRSIQKLLDVDDEDSEWHLQA
                     SQSLNRAYGEEEPDYESIVVREPNPEYR"
     gene            7945..>8196
                     /locus_tag="PK198_12955"
     CDS             7945..>8196
                     /locus_tag="PK198_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF39691.1"
                     /translation="MFAPNLFENQIALVTGGGSGIGYAIAEQLLRLGAKVYIASRKQD
                     RIDKAAEQLRAIGPCEGLVCDIRNTEDLQRAAETIRANEG"
     assembly_gap    8197..8217
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8218..8725
                     /locus_tag="PK198_12960"
     CDS             <8218..8725
                     /locus_tag="PK198_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF39692.1"
                     /translation="LINNAGGQFPSPAEDISEKGWLAVVNNNLNGTWNATHTMAKAFF
                     IPQQSGSIVNIIVNFYRGVPGMAHTGAARAGVDNLTKSLSVEWSKYNIRVNAVAPGII
                     QSSGLENYPPQLLQGIADKIPMKRLGSVQETAWPVVFLVSPMASYITGETLYVDGGSR
                     LWGDFFPL"
     gene            complement(8722..9165)
                     /gene="msrB"
                     /locus_tag="PK198_12965"
     CDS             complement(8722..9165)
                     /gene="msrB"
                     /locus_tag="PK198_12965"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRF39693.1"
                     /translation="MMTWKDVLNYASNGNPAPDKRVEKTKAEWQQQLTKEQYYVTREH
                     GTESPFSNELCYKHDPGLYACICCGSLLFDANVKFDSGTGWPSFTTPYQENSVKYIKD
                     SSFGMVRIETQCNVCDAHLGHVFPDGPEPSGLRYCINGVALKKVD"
BASE COUNT         2228 a         2150 c         2274 g         2040 t
ORIGIN      
        1 ttcggcgatg gtttggctgc cctgtaactc agctaccatt gcccggcaat accggctgct
       61 gctggatcgc tacgctgctg aaacttctac ccagcccgat tttgtggact ggcaggccca
      121 cgatttttcc atgcgcggca tgggcggtat agaaggcgcc ctgctttccg gcgcggcaca
      181 tttgctcagt ttcaccggca cagataccat tcccgccatt gatttgctgg agcaatacta
      241 cggcgccgat gcagaaagag agcttatcgg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaggacatt gccggccgca acggcaaggt
      481 cgtgatccgg cccgacagcg gcgatcccgt gctcattctg tgcggcaacc cggacggcgc
      541 taccgaaccc gaacgcaagg gcgtggtgga attgctgtgg gatactttcg gcgggcagat
      601 caacagcaag ggatacaaag agcttgcctc ctgcattggt actatttatg gagacagcat
      661 tacattggag cgtgcggaac aaatatgtga acgactgaaa atcaagggct ttgcatctac
      721 caatgtagtg ctgggtatcg gttcttttac ctatcagtac aatacgcgag acactttcgg
      781 ttttgccatg aaggccactt atggagaagt gaatggtgag ggaagggcta tttttaaaga
      841 ccccgtgacg gacgacggta ctaaaaaatc ggccaaaggg ctgatgcagc tacggaagga
      901 aaacggccgg tatcatctgt tggatgaagt gagccgggag gaggaaaagg aaggtgaatt
      961 gaaggaagtc tttcgtgacg ggaagctgct gacagaattc agcttagctg aaatcagaaa
     1021 gaacatacgg catcaggggg tataaaaaaa agaagtcgca caggttgtgt gcgacttgct
     1081 gcaataatta taattccacg aatatgaaaa aaaaagaaaa tccacaattt gaaagtctga
     1141 tctaagaacg taatcaaccg ctaatatagt gcgaaaactc gtatataaaa gctgattatt
     1201 ctttgatgac ttcaatcttt ttgttgagcc gcccttccgg agtcactacg ctgagtacat
     1261 agatgccgtt ggccaactgg cttgtgccga tttgtgcgtt gcgagccttc atgctgccgg
     1321 aattgtaaac ttcctgcccc atgaggttat acagcacgat gcgttcaaac tctttacctc
     1381 cgttgaggtg gatattcagg aagtcgctgg tcggattcgg ccatgcttta agcaggtctt
     1441 cggtaagggg cgcttccggt ttttgcaggc tgattttgat gttcaatttt tcaatttcca
     1501 caccataagt ttgcccgtcg ctgcccataa cggtggagac gccgccgccc acttctacgg
     1561 aaagttcttc ctcgctgagg cggatgccgg cgatgtcgtc gcggataatg aagcgtaccg
     1621 ttccaatctc gccatggccg ctggctgcac gagcagtggt gcgcgtaaag ccgctttcca
     1681 aacgcccttc catattatta tggttcataa acaaaatggg cgagttgtaa gcaagccaac
     1741 ctctgtgtga gaaggaagtt tgcacgcttg cgggcgagaa aatattagga ttgtacaaga
     1801 acgggaaggt aaatccatat acgttcacag ccggtttggc ggaggtgcct aatatcattt
     1861 tcaattcgac cacatcgccg gggccagcaa agatgttgcc gctcaggtga attgtctggt
     1921 cggtgagcgg ggctttcgaa acagtgatag agtgtgtacg gccataaaaa ttatggatgg
     1981 cggcagtatc taaggcagag acataaccat tgccgtcggc atccaaatgt ttgagatcca
     2041 cattataatt gacaaacggg ttgtcccagt tttcactgtg tttgccatac caaagcgccg
     2101 ggctgttatc ggggcgcggc aggacggctt cgcccatgaa caagccaata ggcaggaggt
     2161 cttccatgtt gacaacgccg tcgaagttgg catcgccggc ccagatacaa tcgccgaaac
     2221 acataggctc atttccagcg ccaatgtcga ggatgtcaat ttcaattttt acagtgcgca
     2281 tgaaagaaca gccggctgaa ccgttgctgt tgggaatgca ataggcaatt tcgaattcat
     2341 cttcgcctgc attcacagta gcgtggggga catacaccac catgttgtag cctttgatgg
     2401 aacgccccaa aatgaccgtg tccacttcgc cttgcaggag tagaacgctg ccgttgtccg
     2461 gctgatctgt gacgaaaaac tggaagttcg gaacaggaac attgttgcca atgaccaaag
     2521 gagtcgcttt gggcgtcacc attcgataag tggcggcaga aggagcaaaa ttgcttacaa
     2581 aaacttttgc agtggcagtc tccaaattgg atgcgccaaa aggacgacgg gaagaatatt
     2641 tgaaagaatc cgtaccgtaa tagccgggat tgggcgtgta agtaaccaag ccattttcgg
     2701 ggccgagtac gagcgtaccg ttggcaggag acgtcacgat ggtaaacata gtagctgccg
     2761 ttccaaaagc atcgttttcc aatacattga attctaccgg tacgccgggg gtggtatagg
     2821 ctgcatcgtc tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn ntcggggcca ctgtcggaat acacaccgga agagggacct gaaatgcgct
     3001 ggaaccccgg aggtatcaat atcacctggg gagtagcttt gcgtgtgaag acgaggacgg
     3061 tatcggatgc agtcgtattt tctccattga ccacatggac gcttacgttg ccgttgtcgc
     3121 aattgttttc gtcgtcgcaa acgatgtagt tgagcaaagc gacgccttcg aagcccggag
     3181 cgggtgtgaa ttgaatattg ggcgtacctg gcacgaaagt tgcagtaccg ttattgaccg
     3241 atgaaatgct gtttaggata agtatgccgc gactgctgaa atcgttgctt aaaacattga
     3301 aagtcacagg cgtattcaca ctcgtactta cgttgtcctg ccgggcatcc actcttgcag
     3361 gtactacggt tatgtagaaa tcaaggtatt gaaagcaggt tgggatgggg caggtccacc
     3421 gactgacgcg cacaaagtcc tgcccgataa aattgagttg aggagtgtac gtcaaacgat
     3481 aaccattgag cgtaccggag ggcgccaata cggctgtgcc gtgctgggct tgtaccacaa
     3541 cgcccggcgc attgggagaa gaattgaaaa catactccgc cggagtgttt tcaacggttt
     3601 ccagcaaaat actcgtctgc ccctgaagta tcaaagggac ggatagtata agtagctgat
     3661 aaagaatcct ttgtaaaaat acattcatgg ttcgtggatt ttcttcattg cagaattaaa
     3721 acagtttctc tagtatgcag taaatttggt gcaagttagc gaaaaaagat tcaaatgcgc
     3781 aacaccaaat tgtattcaat tttagaacat tttgacaaat atgagcagaa tcgcctgcgg
     3841 aaatatttgc aatctcccta ttttaataga aatcaaacac ttatagatct gttcgatctg
     3901 ttggtggagg atgccaattc tgaaaaaaag aaagagattg aaaaagaaga cgtgtggaca
     3961 ttgttgagac taaagtcgcc ttacgatgat gttcgtttcc gaaagtacta ttccgatttg
     4021 ttgaaactcg tggagggctt tcttgcgcag caggtatatg aggccgaccc ggtgcggcaa
     4081 gccaccgatt tgcttaaagc ggtgggcaag aaaaaaatgg aaaagctata caacagctcg
     4141 gtcaaaacgg ctcgccatct ttcccaaaac caaccctggc gccatgcaca gcactatctt
     4201 cagcagtatc agatagaggt caatttttac gacatcatgg cctttgaaac caagcggggc
     4261 gcgcgcacca atctggaaga aattgccaac aatttagact ccttctattt ggctgaaaaa
     4321 ctccgtattt acgcttccgt acttacacag caaacctttg tctctcagga atataaggtg
     4381 aggttgattg atgaaatcat ggcctacctg catgcccata aagaccatga ggagacgcct
     4441 gcagttgctg tttattttca ggtattgcgc accgtcaccg accaagacaa tctggagcat
     4501 tattacagac tgaaggagct gttggatcag tacggcctgt tgtttcccaa acatgaggcg
     4561 atggagttgt acgggtcggc tttgaactac tgcattcgtc agatcaacca cggtaatcaa
     4621 ggctttctgg aagaactctt tattttgtat aatcagttga tagacaagga gatcatcttc
     4681 ctgaacgacg agttttcgcc ctgggacttc cgcaatatcg tcgttatcgc gctgcgtttg
     4741 ggacgctacg aatgggtgga gaaatttatc catgacttca accacaaaat cccggaattg
     4801 aaccgcgaca acaccgtaac atatacgctg gctcagttgt atttctacca aaaaaaatac
     4861 gacaaagtca tcgagcagtt gcgcaacgtg gaatacgacg acgtttcata caacctcaac
     4921 tccaaaacca tgctcctgat gacctatttt gaaaccgatg agtacgaacc cctgtactct
     4981 ctgttcgaga gtttccgcac ttatctgaat cgtcaaaaag agttgtcttc cgacagaaaa
     5041 gcactgtata aaaaccttat caaattcacc aaaaaactca ccaaaatcat gccgggcgac
     5101 aaggcggagg tcaagcggct caaggaggaa atagccgcta ccggcaatgt ggccagcctc
     5161 aactggatac aagaaaaaat tgaggaattg gtatannnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnc aaatggctat tgcacagcct gttattcaat tgcaaacttt cgctaccggc
     5401 ttcacgcgcc cggtggatat tgcttccgcc ggagacgaac gcctctttat tgccgaacag
     5461 cgcggcatca tccacatcct caatgcgcag ggccaacgcc tgccgaccgc gtttttgaac
     5521 atccaaagcc gcgtcaactc cgcctccaat gagcgcggcc tgctgggctt ggtttttcat
     5581 cccaactacg cccaaaacgg ctacttctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccctattc caaccacaat
     5761 gccggcgacc tcaacttcgg ccccgacggc tacctgtact tcggcctcgg tgacggcggt
     5821 tcgggcggcg acccacagaa ctacagccaa aacagacagt cttacctcgg caagatgatg
     5881 cgcatagatg tcaacagcgg tagcccgtat gcgataccgg ccaccaatcc gtttgtgaac
     5941 gatccttcta cgctcaatga aatttgggct atcggcttgc gcaatccctg gaaattcagc
     6001 ttcgaccggc tgacgggcga catgtggatc ggcgatgtgg gccagaatgc ctgggaggaa
     6061 attgatttcc aaccggcggc gagcatcggc ggtgaaaatt atggttggcg ctgctacgag
     6121 ggcaatgcta cctacaacac ggcgggctgc cagcctgcta cggcttataa gtttcccatt
     6181 catgtttatg tcaacggatt aaactccggc tgctcggtta ccggtggctt tgtgtatcgg
     6241 ggcagcgagt tccccgactt gtacggacac tatttataca ccgattattg cggcggtaaa
     6301 atctggtcgt tgacgcccga tggaaacggc ggctggacga accgccaact gctgcaaggt
     6361 gctgccaatc aatatgtttc atttggtgaa aaccaaaacg gcgagttgtt tctggcggca
     6421 atcggggtgg gtactatttt gagaatcaga gaaatttgct ccacttttca ggtggcaggc
     6481 acggcgaccg acgaaacttg cgcaggtact gccaacggaa gcatccaact caatatcacc
     6541 aatccgggcg ctacgcaaac catcgcctgg tcgaacgggg ccaataccgg caccatcagc
     6601 aacctcgctg ccggaactta taccgtgacg gtgacgagtt ccaacacctg tgcgcgcacg
     6661 ctgacttata cgctgacaaa tggctcgcct gccgcgccga atttgtcgtt ggctcccgta
     6721 gaaatatgca ccggtgcatc tgcttcactc gaagcgcccg cagcgcctgc cgggctgggc
     6781 tatcagtggt atcgcaatgg ccaaccggtt cctggagcta cacagcgaat cctcttggtc
     6841 acagaagcgg gcagttacac ggtagcattt acggtcacgg taggttgcgc ttcggcacag
     6901 tccaatcccg ttgtcgtcac cgtgtcggtt tttccgacat tgccgcccat caccgcagaa
     6961 ggaaatgtat tgacggcgcc cgacctcgac gtatttattc tttaccaatg gctgctcaac
     7021 ggccaaccca tcgctaatgc caacggcctg atttacacgg cgcttgaatc gggtgagtac
     7081 tcggttcagg ctacgaccct gaacggctgc acctacacct ggcccgctgt gacggtggtg
     7141 gtgagcagca cagccgaaat tcggggcttg cagcgctttg tcgtatctcc caatccggtg
     7201 cgcgatatgc tggccataga gcttcagttg gaatcttccg ccccaatgac gctccggctc
     7261 accaatgcgg ctggcaaagt agtgtgggaa cagcgggaac gcataggcgg tctgtggcgt
     7321 actgagttgg atatgagccg tttacctgcc ggtatgtatt cactgacact gagccgcagc
     7381 cgccgcgagc aggccgtggt acagttggtg aaacagtaag gggcgataaa aaaataaagt
     7441 gatcattttc gccaaaggat tagataagta aacgtttcat tttaagtgtc ttatctaatt
     7501 ctttggccta aagaaaatga gcatttattt ttgccccact actaagaggg ggtgggtgtt
     7561 ttttttgtgt aaaaaataat tggctatgac tgacatcaat gctgttaaat ccagaatcat
     7621 ccagaggatt caagagacag atgaatactg ggtgatcaga agtattcaaa aattattgga
     7681 tgtggatgac gaggactccg aatggcactt gcaggcttcg cagtctctca atcgtgctta
     7741 tggcgaagaa gagcctgact atgaatctat tgtagtaagg gaacccaatc ctgaataccg
     7801 atgaaaggag gcgagatcgt atttgaacaa actcctgaga agactcgcgg attacatctc
     7861 aaaatcagca tactgaaata gaagtcaaaa gtgtggcctg ccgcctacaa caggcgagta
     7921 gtcatcaatt aatcaactca actcatgttt gcacccaatc tgtttgaaaa tcaaatcgcc
     7981 ctcgtcaccg gcgggggcag cggcatcgga tacgccattg cagagcaact actccgtttg
     8041 ggggccaagg tttacatcgc ttcccgcaag caggaccgca tagacaaagc cgccgagcaa
     8101 ttgcgcgcca tcggcccctg cgagggcttg gtctgcgata tccgcaacac cgaagacctg
     8161 caacgcgccg ccgaaaccat cagagccaat gagggcnnnn nnnnnnnnnn nnnnnnnact
     8221 catcaacaac gcgggcggtc agtttccatc ccctgcagaa gacatcagcg aaaagggctg
     8281 gttggctgtg gtaaacaaca acctcaacgg cacctggaac gctacccaca ccatggccaa
     8341 agcgtttttt ataccgcagc aatcggggag cattgtcaac atcatcgtca atttttacag
     8401 gggtgtgccg ggcatggcgc acaccggagc cgcccgcgcc ggcgtggaca acctcaccaa
     8461 atcactgtcg gtagagtgga gcaagtacaa catccgagtg aacgccgtgg cgccgggtat
     8521 catacaaagc agcggcctgg aaaactaccc gccgcaattg ttgcagggca tagccgataa
     8581 gattccgatg aagcgcctcg gcagcgtgca ggaaaccgcc tggccagtgg tattcctcgt
     8641 gtcgcccatg gcctcgtaca tcaccggcga aacgctgtat gtggacggcg gcagccgcct
     8701 gtggggagac ttttttccgc tttagtccac cttttttagc gccacgccat tgatgcaata
     8761 gcgcaggccg ctcggctcag gaccatcggg gaacacatgc cccaggtgag cgtcgcagac
     8821 attgcattgc gtttcaatgc gcaccatgcc gaaggagctg tctttgatgt acttcacgct
     8881 gttttcctga taaggcgtgg taaaactggg ccatccggtg ccggaatcga acttcacatt
     8941 ggcgtcaaac agcaggctgc cgcagcaaat acaggcgtac aaacccggat cgtgtttgta
     9001 acacaactca ttgctgaacg gcgattctgt gccgtgctcg cgggtgacgt aatactgctc
     9061 tttggtgagt tgttgctgcc actcggcctt ggttttttct acccgtttgt cgggcgcagg
     9121 attgccgttg gaggcgtaat tcaggacgtc tttccaggtc atcatggttt gttttgtttt
     9181 gtccgtcttt tcagaagatg tgctgtcggg ccgcgcagat gctgctgttc gggaattgca
     9241 ggcaggcaag gccgtcagcg agcaagtcaa aagaacaagc cccgcaggca ggcg
//
LOCUS       DAORLF010000153         9237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4034_length_9237_cov_1.918101, whole genome shotgun sequence.
ACCESSION   DAORLF010000153 DAORLF010000000
VERSION     DAORLF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4034_length_9237_cov_1.918101"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..844)
                     /locus_tag="PK198_12970"
     CDS             complement(56..844)
                     /locus_tag="PK198_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRF39694.1"
                     /translation="MQYQPQLDFLLRQARKAADIMRLRFRGDFQVYIKPDGSKVTDVD
                     LEISKMWQADRAAEFPESGLYSEESPDKTILPGKDYFVVDELDGTSYFADGQHGFSHQ
                     AAYFVPGEGLVIGVLAYPLDDILLYAVHGEGVYLEQNGQTERIEPVRPKGKDELFFGH
                     PARYKGEKYYDLLTALGIPPQRILQTNAMRTLQMARRELDVNIFLMRKIDAWDWAGEK
                     VIVEELGLNHSYLDGAPIHFGQPPREDNPGYLLCPKEYVEWLAR"
     gene            complement(943..>1484)
                     /locus_tag="PK198_12975"
     CDS             complement(943..>1484)
                     /locus_tag="PK198_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39695.1"
                     /translation="TARNIFEGGAKLLQYPFVAWSEQRIHFREFEDGSVASKSFNNQA
                     FTSDLYRLGVKIFMDVNSFGDSTNMLTATIFDPYESRYRLPQTPVALCFINIYFDLME
                     IERRDLILEIAASQKHISEIKQAYRQHQEHWSALSRRYFNEVQRGENLENLESWNQRV
                     VAHLGIDNMAILGLKPSEK"
     assembly_gap    1485..1583
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1584..>2362)
                     /locus_tag="PK198_12980"
     CDS             complement(<1584..>2362)
                     /locus_tag="PK198_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39696.1"
                     /translation="ADWFDLLNACRKRQSKIIKPSKAYFELHSTLDNAQIELVEGYFN
                     AEIQSYDLIGLEMKAGRLAVQASGERLFVSMESSRAVTMHRLFEISDVFPDSPLLLNR
                     KQLKIQYSISMSKRFVNENADTVIVLDFVPKKSQGRLFSGEIWINASRKNILKIEQHC
                     AAAQRYPFRPLFPHDSIRNIDISITKTFREQGSEAFFNHVDFSYTVDYQDRKSQKYQV
                     HTQAVLYAYAPDEVFDLPEFHFSSDSIGDYRKINAFPYNDF"
     assembly_gap    2363..2383
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2384..2750)
                     /locus_tag="PK198_12985"
     CDS             complement(<2384..2750)
                     /locus_tag="PK198_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39697.1"
                     /translation="MKRSILPRKSAVRNWLLFLIAAAGSWHTYGQTTIEGRILSDDSK
                     APLAYATIYNKTTGKGAISDELGHFQITVTSAQDEVVASYVGYLKQRIELQTGKVKYE
                     VKLRENPAMLGEVEVVGTWE"
     gene            2972..>5776
                     /locus_tag="PK198_12990"
     CDS             2972..>5776
                     /locus_tag="PK198_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF39698.1"
                     /translation="MKQTLLRVNLVFIGLICFSATLLAQMRVAGTVKDAAGEPLIGAS
                     LIVKGTTTGTITDVDGSFSLEVPGKSATIEVTYTGYRSKTVQLTADNTAMAIVLEDDV
                     ARLDEVLVTGLASGVKRANSGNAVTSITGDELVGATNPQTLDNALFGKIPGVNMSSNS
                     GAPGGGVNVQLRGVSTLGAGSSQPLYIIDGVYVDNSTIRNGRTQVNGASGGASVATQD
                     DAANRIADINPDDIEKIEVLKGPSAAAIYGTRANSGVIIITTKRGAAGKTKVSLSQDL
                     GMGQGQNFQNFDTWNEAKITNFFSGARQQLELQRFQAAKQAGRENTNWEEFFYGENAL
                     LSNTQLSISGGNDKTQFFASGGVLSEDGIIKNTGFERYTMRANLDQKLGNRLKISVSN
                     NYTRSNNDRGFTGNQNNTGGSIGYALAYTPSYADLLPDATGNYPNNPYFNDNPVAIRD
                     LGTNNQKVDRFISAGTVEWDILQNTSSFLKLRVNGGVDYLSSNTIVHFPEVLQHQRAQ
                     GNQAGDVMWGAQDNLNTNLQAFLIYNADLGSVNSNTAIGISRLDQQSNFLLTRGRGLN
                     GGQTNLTWARVQAIQGQVNSTVTDVGLVAQQDLNWQDRITASVGIRLDKSTLNAQQDK
                     YYAFPKASLALNLANFDFWNVGIVNQFKLRAAYGQTGGLPNFGVTFESLAPQIIGGLT
                     GGQVGTRGVDPNLTPETAEELEFGLDAAFLKNRITIEATYYNKGVKNLILDLQPAEST
                     GILAIATNAADLENKGIELGIGATPVRTSKFNWYTKLMYWQNRSVITRLDIPVFTTGG
                     FGASLGTYLISEGYSPTTIVGNPVVANSPQTSGRTVYGDRQPDFQMSWLNSITFLKNF
                     DLNWLFHYQKGGESINLSALLWDDGGTTNGWSNDSDGDGTPDGLRRLTAWSGGNTGVW
                     IEKTDYIKLREVALYYTLPKD"
     assembly_gap    5777..5881
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5993..>6832
                     /locus_tag="PK198_12995"
     CDS             5993..>6832
                     /locus_tag="PK198_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF39699.1"
                     /translation="MKSIFQKIISALSVVLLLTLGACEIDLVTDPNNPSLGSALNDAK
                     KPELQVLVTGLEARHRGYVQNAVEMFGREVWAYFGSDPRFANEWLGLSIAQTYPDFFS
                     SGGTYVNPYLAVKQANVLIRAAENSTALTDAERKGYIGFAKTIKGFQLLWPLMQQYEN
                     GIRIDVEDPLNPGPTLPFDQAIPAEHLAAQLGRQLLGRRLIGKGRRALGHGGHTDLLA
                     AGSTFSFNLTSGFAGFNTPDKMAQVNRAIAARAALYAKDYSGALNTLNGSFMDLNVTD
                     VTFE"
     assembly_gap    6833..7041
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7042..7432
                     /locus_tag="PK198_13000"
     CDS             <7042..7432
                     /locus_tag="PK198_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF39700.1"
                     /translation="GGNLLLGEYQDARWPSNTTAFKFIRNEELILIYAEASLLTNNTA
                     NAVSAINTIRNTWGVGNYTGATTQDALLNEILFQRRYSLWAEGGHRWIDLRRTNRLNN
                     TYVDLRDRGNIFTQVARRTSEINWDAR"
     gene            7760..8383
                     /locus_tag="PK198_13005"
     CDS             7760..8383
                     /locus_tag="PK198_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF39701.1"
                     /translation="MNKQSLQSLLRKRFPTLGDESLAAFLNICEYRAVKKKEIILPAG
                     STQRTAFYVLEGFVRGILTGENNKERNLMLRMEGSFNLSVNQLMEAAPGRYGFEAISA
                     CKLLVFSFEAFETLAQNHPDFGRLYVNILKENILILQSRVDEMATKQPEQRYLTLLEQ
                     NPAFFQSAFNKHIANFLGITPVSLSRIRKRIYDKYKNRADEPELPAM"
     gene            8502..>8850
                     /locus_tag="PK198_13010"
     CDS             8502..>8850
                     /locus_tag="PK198_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39702.1"
                     /translation="MDHPIIQFIADKHMFFVVGILVFFGLIETALGYYAATKRTKNDL
                     MVEVVNTFFLMVITKPAVVFIAMSALKWLAPAGEGALSGLSFWILLPMFLLVDDFLQY
                     WYHRSSHEYKWLWK"
     assembly_gap    8851..8998
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2131 a         2355 c         2091 g         2078 t
ORIGIN      
        1 caaggtttcg ggttgctcac ccctcacaca ctcacccctc acccctgaaa accgcttacc
       61 gagccaacca ttccacatac tccttggggc acagcagata gccgggatta tcctcgcggg
      121 gcggctgccc gaaatgaata ggagcgccat ccagatagct gtgattcagg cccaattctt
      181 ccacgatgac cttttcgccg gcccaatccc atgcatcaat ttttcgcatg agaaagatat
      241 tgacgtccaa ttcgcggcga gccatttgca gagttcgcat ggcattggtt tgcaggattc
      301 gttgtggcgg tatgcccaat gcagtcagca agtcgtaata tttttctcct ttgtagcgag
      361 ccgggtgccc aaaaaatagt tcgtctttgc ctttgggcct caccggttcg atgcgctccg
      421 tttggccgtt ttgttctaaa tagaccccct cgccgtgcac ggcatacagc aaaatatcat
      481 ccaaaggata cgccaagacc ccaataacca agccttcgcc cggtacgaaa taggccgcct
      541 gatgcgaaaa gccgtgttgg ccgtcggcga aatagctggt gccgtccaac tcatccacca
      601 caaaataatc tttgccggga aggatggtct tgtcgggcga ttcctcgctg tacaggccgg
      661 attcaggaaa ctcggcggcg cgatcagcct gccacatttt agatatttca aggtccacgt
      721 cggttacttt agatccgtcg ggtttgatat acacctgaaa atcgccgcga aagcggaggc
      781 gcatgatgtc ggcggctttt cgggcctggc ggaggaggaa gtcgagttgt ggctggtatt
      841 gcatggcgca ttgaagtttt gaaacagccg gcggtggcat gttattttga aatataattt
      901 cgctgcaaat atagcgggat ttttgaaggc gagaaagcct gcctacttct cggatggttt
      961 caatcccaaa attgccatgt tgtcaatgcc gagatgcgcg accacccgtt gattccacga
     1021 ttccagattt tcgaggtttt cacctcgttg tacttcattg aaataccgcc ggctcaaagc
     1081 agaccaatgc tcctgatgtt gccgataagc ctgttttatt tcggaaatat gcttctggga
     1141 ggccgcaatt tcaaggataa gatcgcgccg ttctatttcc atgagatcga agtaaatgtt
     1201 tataaaacac agggcaacag gtgtttgtgg caagcggtat ctgctttcat aaggatcaaa
     1261 aatagtagcc gtaagcatat tggtggaatc cccgaatgaa ttgacatcca tgaatatttt
     1321 tactcccagc cggtacaaat cggaagtaaa tgcctgatta ttaaaggatt ttgaagctac
     1381 ggagccatct tcaaattccc ggaaatgaat gcgttgttca gaccatgcca caaaaggata
     1441 ttgtaacaac ttggcgccgc cctcgaagat gtttctggcg gtgannnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnaaaaagt cgttgtaagg aaaagcattg attttgcgat
     1621 agtcgcctat gctgtcgctg ctgaaatgaa attccggcaa atcaaaaacc tcatccggag
     1681 cgtaagcata caaaacagcc tgtgtatgaa cctggtattt ctgcgatttg cgatcctgat
     1741 agtcaactgt gtaagaaaaa tctacatgat tgaaaaaagc ctccgagcct tgttctctga
     1801 aggttttagt gattgaaata tctatgttgc gaatagaatc gtgagggaac agcggcctga
     1861 agggatagcg ttgtgcagcg gcgcaatgtt gctcaatttt tagaatattt ttgcgggagg
     1921 cattgatcca gatttcgcct gaaaagagcc ggccttgaga ttttttgggt acaaaatcca
     1981 atacgatgac ggtatcggca ttttcgttta cgaaccgctt ggacatactt atggaatatt
     2041 gaattttcag ttgctttctg tttaggagca ggggactgtc ggggaataca tcgcttatct
     2101 caaaaagccg gtgcatcgtg acggcacgag agctttccat agacacaaaa agccgctctc
     2161 cggaggcttg aaccgccagc cggccggctt tcatctctaa tccaatgaga tcatagctct
     2221 gaatttctgc attaaaatag ccttccacca gctctatctg agcattatcc aatgtggaat
     2281 gcagctcgaa gtaagccttc gatggtttga ttattttaga ttggcgtttc cggcaagcgt
     2341 tgagcaaatc gaaccagtca gcnnnnnnnn nnnnnnnnnn nnncctccca tgttcctacc
     2401 acttctactt ctcccagcat ggctggattt tctctcagtt tcacttcgta ttttactttg
     2461 ccggtctgta gttcaatgcg ctgtttcagg taaccgacat aagacgccac cacttcatcc
     2521 tgcgctgatg ttaccgttat ctgaaaatgc cccagttcgt cgctgatagc gccttttccg
     2581 gtggttttat tataaatggt tgcataagcc agaggtgctt tggagtcatc gctcagtatg
     2641 cggccctcaa tggtcgtttg cccgtaagtg tgccaactgc cggcggcggc gatgaggaac
     2701 aagagccaat tgcgcactgc cgattttcga ggtaggatgc ttcttttcat aaagtattgc
     2761 cgaactggtt tcacaacaaa aatagctggt tctaaactgc ttctgcgcac cgaatcaatt
     2821 ttcgtcggca ggccgcgaat tttcgcctta ttcgttagga aatatacctc aaaattttgt
     2881 tgagaacttt gcattaactt gcgaatatta tcgccatttt tgagccgact ttagattcat
     2941 tatcatttcc tcactaaaaa ctcgtgattt tatgaagcaa accttactca gggtcaacct
     3001 cgtttttatc gggttgattt gcttttcagc aacccttttg gcacagatgc gcgtggcagg
     3061 caccgtcaag gatgcagccg gcgaaccact catcggcgct tccctcatag tgaagggcac
     3121 caccacaggt accatcactg atgtggatgg cagtttttca ttggaagtgc ccggcaaatc
     3181 cgccaccatt gaggtaacct acaccggcta tcgcagcaaa accgtgcagc tcactgccga
     3241 caacacagcc atggccattg tgttggaaga cgacgtggcc cgcctcgatg aggtactcgt
     3301 taccggtctg gcatcggggg tcaaacgagc caactcaggc aacgccgtga cctctatcac
     3361 cggcgatgaa cttgtgggcg ccaccaatcc acaaacgctt gacaatgcgc tgtttgggaa
     3421 aattcccggc gtcaacatgt cttccaacag cggtgcgccc ggcggcggcg tcaacgtaca
     3481 gttgcgcggt gtttccaccc tcggcgcagg ctcctcgcag ccgctgtaca tcattgacgg
     3541 cgtgtatgtg gacaactcca ccatccgcaa cggtcgtacg caggtcaatg gggccagcgg
     3601 cggcgccagt gtagccactc aggacgatgc agccaaccgc attgccgata ttaaccccga
     3661 cgatattgaa aaaatagagg ttttgaaagg cccttccgca gccgccattt atggtacgcg
     3721 ggccaattcg ggcgttatca tcatcactac caaacggggt gcggcaggca aaacaaaagt
     3781 cagcctttcc caagacctcg gtatggggca aggccagaat ttccaaaact tcgacacttg
     3841 gaatgaagcc aaaatcacca atttcttctc cggcgcccgc cagcaattgg aactgcaacg
     3901 ctttcaggca gccaaacaag ccggcaggga aaataccaac tgggaggagt tcttctatgg
     3961 cgagaacgcc ctgttgtcca acacgcagtt gagcatcagc ggcggcaatg acaaaacgca
     4021 attcttcgct tccggcggcg tactctcaga ggatggaatc atcaaaaaca caggcttcga
     4081 gcgctacacc atgcgcgcca atttggacca aaaattaggc aatcgcctca aaatttcagt
     4141 cagcaacaac tacacgcgct ccaacaatga ccgcggcttc accggtaacc aaaacaacac
     4201 cggcggcagt atcggttacg ccctggccta tacgcccagc tacgccgacc tcctgcctga
     4261 tgctaccggc aactatccca acaatccata cttcaacgac aacccggtgg ccatccgcga
     4321 cctcggtacc aacaaccaga aggtggatcg cttcatctct gcaggcaccg ttgaatggga
     4381 tattcttcaa aatacctcct cttttctcaa actgcgcgtc aacggcggcg tggactacct
     4441 gagttccaac accatcgtcc atttcccgga agtgctccag caccagcgcg cacagggcaa
     4501 ccaagccggc gacgtgatgt ggggcgcaca ggataatctc aatacgaacc tccaggcttt
     4561 ccttatttac aacgccgatc tgggttccgt caactccaat acggccatcg gcatcagccg
     4621 cttagaccag caatccaact tcctgctcac tcgcggccgg ggtctcaacg gcggccaaac
     4681 caatctcacc tgggccagag tacaagccat tcaggggcag gtgaattcaa ccgtaaccga
     4741 cgtgggcctc gtagcacaac aggacctcaa ctggcaagac cgcatcacgg cctctgtggg
     4801 cattcgcttg gacaaatcca cgctcaatgc gcagcaagac aagtattacg cctttccgaa
     4861 agcatctttg gcgctcaatc tggccaattt tgatttttgg aatgtgggta ttgtcaatca
     4921 attcaaattg cgcgcagctt acggccagac cggcggtttg cccaatttcg gcgtgacgtt
     4981 tgaatcgctg gcgccgcaaa tcatcggcgg cctcaccggc gggcaagtgg gcactcgcgg
     5041 tgtggacccc aaccttacgc cggagacagc agaggaactg gagtttggct tggatgctgc
     5101 ttttctcaaa aaccgcatca ccatcgaggc cacctattac aacaaaggtg tgaaaaacct
     5161 catcctcgac cttcaaccgg ctgaatctac gggtatcctc gccattgcca ccaatgccgc
     5221 cgaccttgag aacaaaggta tcgaattggg tatcggcgct acgcccgtgc gcacatctaa
     5281 gttcaactgg tacaccaaac tcatgtactg gcaaaaccgc tctgtcatca cccgcttaga
     5341 tattcctgtg ttcactaccg gcggtttcgg ggcgtctttg ggtacttacc tgatttcgga
     5401 agggtattcg cccacgacca tcgtaggcaa cccggtagtg gccaacagcc cgcaaaccag
     5461 cggacgcacc gtgtatggcg accgccagcc cgactttcag atgtcatggc tcaactccat
     5521 cactttcctc aaaaacttcg acctgaactg gcttttccat taccagaaag gcggcgagtc
     5581 catcaacctt tccgccctgc tgtgggatga cggcggcacc accaacggat ggagcaacga
     5641 cagcgacggc gatggaacac ccgacggcct ccggcgtctc accgcttggt cgggcggcaa
     5701 taccggtgtt tggattgaga agaccgatta catcaagttg cgggaggtag ccctgtatta
     5761 caccttgccc aaagacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nggttcacag cccgtttctt cggccatcga ggtgacgccg tatcccagct cgcgccgtct
     5941 gtttttccat ctcaaattcg atttctaatc tcaccaaaaa agcaatcagc acatgaagtc
     6001 aatatttcaa aaaatcatat ccgcgctctc cgtcgtccta ttgttgacgc tcggcgcctg
     6061 cgaaattgat ctggtcacag atcccaacaa cccgtctttg ggtagtgccc tcaatgatgc
     6121 caaaaaacct gaattgcagg tgttggtaac cggcctggaa gcccgccaca ggggttatgt
     6181 acaaaatgcc gtagagatgt tcggccgcga ggtgtgggcg tatttcggct ccgatccacg
     6241 ttttgccaac gaatggttag gcttgagcat cgcgcaaacg tatcccgatt tttttagttc
     6301 cggcggaacc tatgtcaatc catatctggc tgtaaaacag gctaatgtac tcatcagggc
     6361 cgctgaaaac agcacagccc tcaccgatgc agaacgcaaa gggtatatcg gttttgcaaa
     6421 aaccatcaaa ggattccaac tgctgtggcc cctgatgcaa cagtacgaaa acggcatccg
     6481 catagatgtg gaagacccgc tcaatccagg gccaaccctg ccgttcgacc aggccattcc
     6541 agccgaacac ctcgccgctc agctcggccg ccagttgttg ggccgccgcc tcatcggcaa
     6601 aggccgccgt gccctgggcc atggaggtca taccgacctc ctggctgccg gcagtacgtt
     6661 cagtttcaac ctgacgagtg gttttgcggg ctttaatacc cccgataaaa tggcgcaggt
     6721 caaccgggcc attgcagcca gagcggcttt gtatgccaag gactactccg gcgccctcaa
     6781 taccctcaac ggttccttta tggatttgaa cgttacggat gtaacctttg aannnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn naggcggcaa tctcctgctc ggcgagtatc aggatgcccg
     7081 ctggccaagc aatacaacgg ccttcaagtt tatccgcaat gaagagttga ttctgattta
     7141 tgctgaggcc agcctcttga ccaacaacac cgccaacgcc gtttctgcca tcaacaccat
     7201 ccgcaacacc tggggcgtgg gcaactacac aggcgccacc acacaggatg cgctgctgaa
     7261 cgagattttg ttccagcgtc gctactcgct gtgggcagag ggcggccacc gctggattga
     7321 cctacgccgc accaatcgcc tgaacaacac ttatgtggac ctgcgcgacc gcggcaatat
     7381 ctttacgcag gtagctcgcc gtacctccga aatcaactgg gacgcgaggt aagaactttc
     7441 atagcttttt ctttttcaga atgcccgtcg tctttggatg gcgggcattt tttgtatgcc
     7501 tgccggaggt tctcaacttc ctgcgcgtcc tgtaagattt cgtatctttg caataaaatt
     7561 ttttcatcca ttgtcgccgg ccgcactcag gtgtcagata aaaaatacga tacggaactc
     7621 accgctcacg gcgtgacgga catcctgcga ttggccattg cggtggaggg gaaggaggtg
     7681 gtggtggagc aagcggagta cgattgacgc ccgttcgcta tacaggaatg tcaaaagacc
     7741 tgcacaacaa taatactcaa tgaacaaaca gtcgctccaa tcattgttgc gcaagcgatt
     7801 tcccactctc ggcgatgaaa gcctggctgc ttttttgaac atttgcgaat acagggccgt
     7861 caaaaaaaag gagatcattc taccggcagg ctccacccaa cgcactgcct tttatgtgct
     7921 ggagggcttt gtacgaggga tattgaccgg agaaaacaac aaggaacgta acctgatgct
     7981 gcgcatggag ggcagtttta atctcagtgt aaaccaactc atggaagctg ctccaggcag
     8041 gtatggcttt gaagccatca gcgcctgtaa attactggtt ttctcctttg aggcttttga
     8101 aacattggca cagaaccacc cagattttgg ccgcctctac gtcaatatct tgaaagaaaa
     8161 catcctcatc ttgcaaagca gagtggatga gatggccacc aagcagcccg aacagcgata
     8221 tctgacgctg ctcgaacaga atcccgcatt ttttcaaagc gccttcaaca agcacattgc
     8281 caatttcttg ggcatcaccc cggtgtcctt gtctcgcata cggaaaagga tttacgataa
     8341 atacaaaaac agggcggacg agccggaact cccggctatg taaaaaatga tgccccaaaa
     8401 aattaacctt tgataacgat gcgcttgccg tgcccgcaat ggagatgtac cgcttcacga
     8461 acttgatatt tttgcccctc gtgaaataaa caacagcttt tatggatcat ccaatcattc
     8521 agttcatcgc cgacaagcac atgtttttcg tcgtcggcat cctcgtcttt ttcgggctta
     8581 ttgaaaccgc gctgggttat tacgcagcca ccaaacgcac taagaacgac ctgatggtag
     8641 aggtagtcaa caccttcttt ctgatggtca tcaccaagcc ggcggtagtt ttcattgcca
     8701 tgtctgctct gaaatggttg gcgcctgccg gggaaggcgc gctgagcggg ctgtcgtttt
     8761 ggatactttt gcccatgttt ttgctggtag atgatttcct tcagtattgg tatcatcgct
     8821 cttcgcacga gtacaaatgg ctgtggaagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     9001 tactcaagca ggccattgtc atttcttcgc acagcctggc cacttgggat tcattttttc
     9061 acaaaaacaa agtgttgatg ccggtactca ggggcttgga gcgcattttc atcacaccgg
     9121 catttcacca tcagcaccac gccgtatcga aagtggacgg cataggcaac cccaacggaa
     9181 actacggcaa tatgctctcc atctgggacc agctattcgg taccgctcaa ttcgtcc
//
LOCUS       DAORLF010000154         9235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4036_length_9235_cov_1.873638, whole genome shotgun sequence.
ACCESSION   DAORLF010000154 DAORLF010000000
VERSION     DAORLF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_9235_cov_1.873638"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PK198_13015"
     CDS             <1..1169
                     /locus_tag="PK198_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF39703.1"
                     /translation="FLKNAPELTRTVPFILPVYQVWKLIYFWIGLKLYDLLSGKLSVG
                     STRLLSARRVAELMPSVETQGLRGGVMYYDGQFDDSGMCIALAHTAATYGATVLNYVE
                     AQSFLYSSEGKISGLRLLDRHTGQRYEVSAGAVINATGVFADVLMGADNPEHKPVVKP
                     SRGTHLVVDTSFFEGNHALLIPRTTDGRVLFAVPWMGKVVIGTTDVPVEEATAEPLAG
                     EEEVDFILQNFNQYCRTQLRRSEVLSVFAGLRPLVRMQDLRSTAHLLRDHALIVSESS
                     LITITGGKWTTYRKMAQDAVNKAAAIAALPFKKCPTRRIAVSRPAKEPSPEYALRLHP
                     AYPYTIADVRHAIRHEMALTVEDVLARRLRLQFLDRAAAQEVEERVREELRGVS"
     gene            complement(1405..2736)
                     /locus_tag="PK198_13020"
     CDS             complement(1405..2736)
                     /locus_tag="PK198_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF39704.1"
                     /translation="MHPQLLLLSLQYLTQAILARLDQYFGADSAAGAMRFPPEPDWAQ
                     YDAAMTQFIQKERLDRDEVLLLLLALAPHIQANYYEAVLLERLPQSGDFPDFGGVKGK
                     QYRGMLPTGETALFVLAGNDLLQRMKVQEYFSAAHPFVRRGILWLEDPHDGEPRMSGR
                     IILSQEYVDLLTTGKVQKPRFSGSFPAELVEIKMDWNDLVLNAQTLSEVKGLLTWLKH
                     GQVLLDDHGMGKRLKPGFRALFHGPPGTGKTLTAGLLGKHTGRDVYKVDLSMVVSKFI
                     GETEKNLANLFDRAENKDWILFFDEADALFGKRTNVRDAHDKYANQEVSFLLQRVESY
                     AGLVILASNFRSNIDEAFSRRFQSIIHFPMPKSNERLILWEKSFPEKFNRNGISLEAL
                     AQKYELSGASILNIVQYASLMALERGDRNILPHDIREGVVREYAKDGKTAP"
     gene            complement(2755..4218)
                     /locus_tag="PK198_13025"
     CDS             complement(2755..4218)
                     /locus_tag="PK198_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRF39705.1"
                     /translation="MKSIFRSGRRRPHQHHEEAQQDNATPFFDKKGDRPRSSPFFSQG
                     GATVQAKLAIGQPGDRYEQEADAVADSVVQNQGRGAAPAIQGKGISRIQRVLTAADSD
                     AATADERMEEDKMVQEKPEVQREAAVAEAMAGEEEEPVQMQHDPLSPRLQRAMEEDVQ
                     MKAEAGGSTAGPQLSNRIKNSAGKGRPLPGKARAEMEQSFGTDFSGVNIHTDTEAMQM
                     NKELGAQAFTHGQDVYFNAGKFSPEHTEGKRLLAHELTHVVQQGGGIRKKSNETEKNA
                     SKPKYPNDFIFYTGGFNGFLGIVQNGQIVYQTTAISGHPGFSEHEKNVGPIPQGKYWV
                     SPNIKRPEVKRPQNGVGGVSGIGSGYQEISSFDLVECDIHSHYCTETCGSQSGTCFTP
                     VGVWGSKRLAIEGRIQVISPDGKKIYRDGFYFHGGHHSVNVTSGCIKVSDSAFFDQVR
                     IHFKSRTPLFVDSQLTSRAILHYFPKVINQFFGYTEY"
     gene            complement(4224..>5041)
                     /locus_tag="PK198_13030"
     CDS             complement(4224..>5041)
                     /locus_tag="PK198_13030"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRF39706.1"
                     /translation="RVGTHLPLPQRHDIGEMKIIAIAIQKGGSGKTTTALNLAAALRE
                     LGKRVLLLDLDPQANLTQALGVSEEIEPNIYHALRQEAFEQAASMEALLVTRHGLDLA
                     PASIELAAVEIELVSIYGREQILKPLLHRLKAPYDFVLIDCPPSMGMLTVNALVASDL
                     IIMPLQAEFLPLKGVRSFMLHLEKIKKLLNPGIDFLGFVLTKYDRRKTMNRNVLEKLK
                     EEYGEEKIFSTFIRTNIALVNAQEKGVDIFTFDKNANGAKDYLQLAQEMLAKM"
     assembly_gap    5042..5186
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5187..>6039)
                     /locus_tag="PK198_13035"
     CDS             complement(<5187..>6039)
                     /locus_tag="PK198_13035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF39707.1"
                     /translation="SQKPDNPAGLGNLSARLVLECREGDKSNQANRFEDWTGYQPINQ
                     PPPVRTKDINHWQPQLVAKKPQKFLTPHWCQVTPFALSKADQFRPPAPVTKEKFAEFK
                     ALGDTVSEYSAKLTETQKLVAEYWAGIHEDRHPGMEYDPSGGYWTTPSGQCCRIAGEI
                     AVKREYGANAVVPLFFALTNALLDASIAAWDAKRYYDYVRPVTMIAELRDDEHFPSWG
                     GPCRGAVEMQGEGWCPYLLGTPPFAEYVSGHSCFSAATAEILRCFTGSNEYGGSVTIP
                     TKGSRIEP"
     assembly_gap    6040..6057
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6058..6412)
                     /locus_tag="PK198_13040"
     CDS             complement(<6058..6412)
                     /locus_tag="PK198_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39708.1"
                     /translation="MSKPYAPPPALRWNQLACEALYLTNTGPTVAARALCIIHTAMYD
                     AWSIFSPGKERSTTTGDMYQRIGDPKWNTAANRRVAYSFAAYRTITCDAIYANPLPDA
                     FKEKVRNYMVKDLVLD"
     assembly_gap    6701..6732
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6781..>7073)
                     /locus_tag="PK198_13045"
     CDS             complement(6781..>7073)
                     /locus_tag="PK198_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39709.1"
                     /translation="GSIGEDNPLTHVETWQVAGNELTWALRNATVRYWAEVDAMGNIT
                     ITYRLNDRLDLSPSPGRREAYNNISRVLGFFYHDLAGGNINLQTRAEWVSRR"
     assembly_gap    7074..7098
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7099..>9235)
                     /locus_tag="PK198_13050"
     CDS             complement(<7099..>9235)
                     /locus_tag="PK198_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39710.1"
                     /translation="AFVDRFGGVISITSSGLVITLPRFCPIPALAHQVDIEPISGDKT
                     VPLFAIPIGPDLFISGEVGVTGTIAPEFQAQIGPICLEGVRMVINPFSHHYGISGSVS
                     ATVAGSLGAEARGGLIGQLSLMAIVPVGPVPVPISVPLTSIEGGVAVMLRGIGTTKWT
                     IGGGLEYGGGAINVGGFSTLELGLAGDAFLGAYGQISLLGENLCRIYWQPFEWHNQIA
                     ATLGIGSNLTIVPGGSSPGVGLSIGGYLGLSPFENFDLAISREGFKDDCPLIETICDI
                     LRVFHKLPSQNNGTWANYGAGNRLEGPLEVYERRPQRASGSMCRGACGPDCDTCKSSP
                     FFVYTDPLSGEVWRYTHFRICDTHEGCREHDAAFDWAADAHGETGPLAIIMPAHMAGN
                     IECACNFPALNCAGWIKGMRPHDGVMLFADMAERASSGISSQPGRGGQPPFGEQGEDF
                     YHYSKNPPAGDFRRGGERWTTYHTNCAAEASAATGVPLPLNYISSIGFGRAINYINVN
                     DFQTRWFNSIITGIYTPANHYRNHQSIPENDYTTQRLTIFSRDCDASSNGNFPTNNNQ
                     LPGQGGETPSQQNLPNQGGGTGDRFNSNSSGQGVTNPTGTNIDLDRVADFSRFQRYGE
                     VLPSSGWLFGEEGIQTTTASNLIELGRASLLNYDIVYLVGNLLSQIRRDPRMVATEEA
                     ILRRLRSDDRFGSQLFYATGTELVGFGGDR"
BASE COUNT         2016 a         2344 c         2334 g         2321 t
ORIGIN      
        1 ttttcttaaa aaacgccccc gaactgaccc gaaccgtgcc tttcattctg ccggtgtatc
       61 aggtttggaa actgatttat ttttggatag gactcaagct gtacgacctg ctgtcgggca
      121 aactcagcgt cggcagcacg cgcctgcttt cggcccggcg agtggcggaa ctgatgcctt
      181 ctgtggaaac gcaggggctg cgcggcgggg tgatgtatta cgacgggcag tttgacgaca
      241 gcggcatgtg cattgccttg gcgcatacgg ccgcgactta cggcgccacc gttttgaatt
      301 atgtggaggc ccaatctttt ctctactctt ccgaaggcaa gatcagcggc ttgcggctat
      361 tggaccgcca caccgggcaa cggtatgagg tctcggcagg ggccgtcatc aatgctaccg
      421 gcgtttttgc cgatgtgctc atgggtgccg acaacccgga gcacaagccc gtggtgaagc
      481 cgtcgagggg tactcatttg gtggtggata cctccttttt cgagggcaac cacgccctgc
      541 tcattccgcg caccaccgac gggcgggtgt tgtttgccgt tccctggatg ggcaaagtcg
      601 tcatcggcac caccgatgtg ccggtggagg aagcaacggc ggaacccctt gccggagaag
      661 aggaggtgga ctttattttg caaaatttca accaatactg tcgcacgcaa ctccgccgct
      721 ccgaagtgtt gtcggtcttc gccggactgc ggcctttggt gcgcatgcaa gacctccgct
      781 ctacggccca tctcctgcgc gatcacgccc tgatcgtttc cgaatcgagc ctcattacca
      841 tcaccggcgg caagtggacg acctaccgga aaatggccca agacgccgtc aacaaagccg
      901 ctgccatcgc cgctttaccc tttaaaaaat gccctacccg tcgcattgcg gtgagtcgcc
      961 cggccaaaga acccagtccg gaatatgcgc tgcggctgca tcccgcttat ccttacacca
     1021 tagcggatgt gcgccacgcc attcgccacg aaatggctct gacggtggag gatgtgctgg
     1081 ctcgccggct gcggctgcag tttttggaca gggcggctgc ccaggaggtg gaggagaggg
     1141 tgagggagga gttgagaggg gtgagttgag aggggtgagt gtgtgagtgg ggtggagtta
     1201 atttcaaaaa ggcaaatcgt tatcttccag gggtatatct gttgaggcca ttgtgatgtt
     1261 ctgataagcg aggccctgtt cttttacgaa ggtgaaaatg tcgggttggc ccggcgggca
     1321 gaggaagtgg ggtagatggt ggcggcaatg tcttggagga gttgtaggag gtagttttgc
     1381 atggggatca atttcgagat ttattcacgg cgccgttttt ccgtctttag catattccct
     1441 caccacaccc tcgcggatgt catgggggag gatattccgg tcgccccgtt ccaaggccat
     1501 gagggaggca tactgaacga tattgagaat ggaggctccg ctgagttcgt atttctgagc
     1561 gagcgcttca agcgaaatcc cattgcgatt gaatttttca gggaatgatt tttcccacag
     1621 gatgagccgc tcgttgcttt tgggcatggg gaagtgaatg atgctttgaa agcggcgact
     1681 gaatgcttcg tctatattgc tgcggaagtt ggaagccagg atgactaagc cggcatagga
     1741 ttcgactctt tgcagcagaa aggacacctc ctggttggcg tatttgtcgt gggcgtcgcg
     1801 cacgttggtg cgcttgccga agagggcgtc tgcttcgtcg aagaatagaa tccagtcttt
     1861 gttttcagcg cgatcgaaaa ggttggctaa gtttttttcg gtttcgccga tgaatttgga
     1921 aaccaccatg gaaagatcca ctttatatac atcgcgcccg gtatgcttac ccaacaagcc
     1981 ggcagtcagg gttttgccgg tacctggggg gccgtgaaac aaggcacgga agccgggctt
     2041 gagccgtttg cccatgccgt ggtcgtcgag cagtacctgt ccgtgtttca gccaggtgag
     2101 cagccccttg acttccgaaa gcgtttgcgc attgagcacg aggtcattcc aatccatttt
     2161 aatttctacc aactccgcgg ggaaggagcc gctgaaccgg ggtttttgca ctttaccggt
     2221 ggtgagcagg tccacatatt cttgtgaaag gatgatgcgc ccgctcatgc ggggttcgcc
     2281 atcatgcggg tcttccagcc atagaatacc tctcctgaca aacggatgcg cagcgctgaa
     2341 gtactcctgc actttcatgc gttggagcag atcattgccg gcaagtacga agagggcggt
     2401 ctcgccggtg ggcaacatgc cccggtactg tttgcctttg acgcctccga aatccgggaa
     2461 atcgccgctt tgcggcaagc gctccaacag cacggcctca taatagttgg cctggatgtg
     2521 cggcgccaaa gccagcaaca atagtaatac ctcgtctcgg tcgagccgct ccttttgtat
     2581 aaactgcgtc attgcagcat catactgcgc ccagtccggc tcaggcggaa agcgcatggc
     2641 gccggctgcc gaatcggccc cgaaatattg atccaagcgg gcgaggatgg cctgcgtcag
     2701 gtattggagt gagaggagga ggagttgggg gtgcatgaaa gagttaataa ttcgtcaata
     2761 ctcagtatat ccgaaaaatt gattaattac cttggggaaa taatggagta tagctctact
     2821 tgtcaactgg ctatcaacaa atagaggcgt tctggattta aagtgaatcc ttacctgatc
     2881 aaagaaagca gaatcggata ctttaataca tccactagtt acattgacag aatgatgccc
     2941 accgtgaaaa tagaatccgt ctctgtatat tttcttccca tcaggagaaa ttacctggat
     3001 ccgcccctca attgccaacc ttttcgatcc ccacacacca acaggagtga aacaagtacc
     3061 agattgagaa ccgcaagttt cggtacagta atggctatga atgtcacatt caacaagatc
     3121 gaaactagaa atctcttgat aaccacttcc aataccactg accccgccga ctccattttg
     3181 aggccgttta acttcaggcc gtttaatatt aggactgacc caatacttac cttgtggtat
     3241 tgggcctaca tttttttcat gttctgagaa tcctggatga cctgagatag ccgtggtttg
     3301 ataaacaatt tgtccatttt gcactatccc taaaaatccg ttgaaacctc cagtatagaa
     3361 aatgaaatca ttaggatatt tgggcttcga agcatttttt tctgtctcat tgctcttttt
     3421 ccgtattcct cccccctgct gcaccacatg cgtcagctca tgcgccagca acctcttccc
     3481 ctccgtatgc tccgggctga acttgcccgc attgaaatac acatcctgcc cgtgagtaaa
     3541 agcctgagcg ccgagttcct tgttcatctg catcgcttcg gtatcggtat ggatgttgac
     3601 cccgctgaaa tccgtgccga acgactgctc catttccgcg cgagccttgc cgggcagcgg
     3661 cctgccctta ccagcggagt tctttatgcg gttgctaagt tgcggccccg ccgtgctgcc
     3721 tccagcttca gctttcattt gaacatcttc ctccatcgcc cgctgtagcc ttggcgaaag
     3781 cgggtcatgt tgcatctgca caggctcttc ctcctcgccc gccatagctt cagcgacggc
     3841 ggcctcccgc tgtacttccg gtttttcctg caccatctta tcctcctcca tgcgctcatc
     3901 ggcggtagct gcgtcgctgt cggcggcggt caaaacccgt tggatacggc tgatgccttt
     3961 accctggatg gccggcgcag cgcctctgcc ctgattttgc accacgctgt cggctacggc
     4021 atcggcctcc tgctcatacc ggtcgcccgg ttggcctatg gcgagcttgg cttgtacagt
     4081 ggctcccccc tgcgaaaaaa acgggctgct tcggggcctg tcgccctttt tatcgaagaa
     4141 aggcgtcgca ttgtcctgct gggcctcttc gtggtgctgg tgcggccggc ggcgccccga
     4201 tcggaatatg cttttcatgg ctgttacatt ttggccaaca tttcttgcgc cagttgcagg
     4261 tagtctttgg cgccgttggc gtttttgtcg aatgtaaaaa tatctacccc cttttcctgg
     4321 gcattcacca aagctatatt ggtgcggata aaggtactga aaatcttctc ttccccgtat
     4381 tcctccttca gcttttccag tacattccgg ttcatggtct tgcgccggtc atacttggtc
     4441 aatacgaatc cgagaaaatc aatgcccgga ttcaacagct ttttgatctt ctccagatgt
     4501 aacatgaaac tgcgtacgcc tttgagcggc aaaaactcgg cttgcaacgg catgatgatc
     4561 agatcgcttg ccaccaaggc gttgaccgtg agcatgccca tggagggcgg gcagtctatc
     4621 agcacaaaat cgtaaggcgc cttcaggcgg tgcagcagcg gtttcaggat ttgttcccgc
     4681 ccatatatgc tcaccaattc gatctccacc gctgccagtt caatggatgc aggcgccaga
     4741 tcgaggccgt gacgggtgac caacaaagcc tccatggacg cggcctgttc gaatgcttcc
     4801 tggcgcagcg cgtggtaaat gttgggttcg atctcctccg acacacccag cgcctgggta
     4861 aggttggcct gtggatccag gtccaacagc aatacacgtt tgcccagttc gcgcagggca
     4921 gcggccagat tcagagcggt cgtggttttg ccggagccgc ccttctgtat cgcaatggcg
     4981 atgattttca tttcgcctat atcgtgccgt tgaggtagcg gcaggtgcgt tcccacacgc
     5041 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnggct ctatgcggga acctttggta ggaatggtca
     5221 cggagccgcc gtattcattg ctgccggtga aacaacgcaa aatctcggcc gtagcagccg
     5281 aaaagcaact atggccggac acatactccg caaatggcgg agtaccaagt aaataggggc
     5341 accagccctc gccctgcatt tccacggcac cgcgacatgg cccgccccag gatgggaaat
     5401 gctcgtcatc gcgcagttcg gcgatcatcg ttacggggcg tacatagtca tagtatcgtt
     5461 tggcatccca ggcggcgatg gaggcatcga gcaaagcatt ggtgagggcg aaaaacaggg
     5521 gcacaaccgc attggcgccg tattcccgct tcactgctat ttcgcctgct atgcggcagc
     5581 attgcccgga gggggtggtc cagtacccgc cggaggggtc atactccatg ccgggatggc
     5641 ggtcttcgtg gatgccggcc cagtattcgg ccacaagttt ttgcgtctcc gtgagtttgg
     5701 cgctgtactc ggacacggta tcaccgagcg ccttgaactc cgcaaatttc tccttcgtta
     5761 caggcgccgg cgggcgaaat tggtccgctt tgctcagggc gaacggcgtc acctgacacc
     5821 aatgcggagt gaggaatttt tgcggttttt tggccaccaa ttgaggttgc cagtggttga
     5881 tgtccttggt gcgcaccgga ggaggttgat tgatgggctg atagccggtc cagtcttcga
     5941 agcgattggc ctggttggac ttgtcgcctt cgcggcattc cagcaccaag cgggcggaca
     6001 ggttgccaag ccctgccgga ttatcgggct tttgagaggn nnnnnnnnnn nnnnnnnggt
     6061 cgaggaccaa gtctttgacc atataattcc gcaccttctc cttgaaggca tccggcagcg
     6121 gattggcgta gatggcatcg caggtgatgg tgcggtaggc agcaaagctg taggcgaccc
     6181 tgcggttggc cgccgtgttc cacttgggat cgccgatgcg ttggtacata tcgccggtag
     6241 tggtgctgcg ctccttgccg ggcgaaaaaa tagaccaggc atcgtacatg gccgtgtgga
     6301 tgatgcacag cgcacgggcg gctacggtcg gccccgtgtt ggtgaggtag agggcctcgc
     6361 aggccaattg gttccagcgc agggcgggag ggggtgcata tggtttggac atggtggtgg
     6421 aaggttttaa agatgagaaa atgggtatgg tatgttcagt tgatgttgtc gcgctcagag
     6481 gttggttgga gggttttctg gtagtggcgg tgcttggagt ggtggatttt acagcctgtg
     6541 gtgtttaaaa tactggctta tagtgcattg tatgtgctta ttggtgatgg aaaatgttgg
     6601 atatgcaggc gggactcgcg tgatagtttg gacgagatgg acttgatacg cgtgtcgggg
     6661 aaggcgcgag gggatgcaaa gtgtgaaatg tttgatatgc nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nncgcacgag atggaatctc ggcgcgagcg ggtatggtgg cggctattta
     6781 ttatctccga gacacccatt ctgctcttgt ttgtaagtta atgtttccac ctgctaaatc
     6841 gtggtaaaaa aagcccaaaa cacgagaaat attattatat gcttctcttc tgccaggcga
     6901 ggggcttaaa tccagcctgt catttaagcg ataagtgatg gtaatatttc ccattgcgtc
     6961 aacttctgcc caatatctta cagtagcatt tcttaaagcc caagtaagct cattaccagc
     7021 tacttgccac gtttcaacat gcgtcaacgg attatcctcg ccaatactcc cccnnnnnnn
     7081 nnnnnnnnnn nnnnnnnncc tatcacctcc gaagccaaca agttcagtac ctgttgcata
     7141 aaaaagttgt gagccaaatc tatcgtcact tctcaacctt ctcaatatcg cttcctctgt
     7201 tgcaaccata cgagggtctc ttcttatttg acttaataag ttcccaacaa gatatacgat
     7261 atcataatta agtaacgaag ctcttcctaa ttcaatcagg ttggatgctg ttgtggtttg
     7321 tatcccttcc tctccaaata gccagccact cgaaggaaga acctcgccat agcgttgaaa
     7381 tctcgaaaag tcagccactc tatccaagtc aatatttgtt ccagttggat ttgttactcc
     7441 ttgtccagat gaattggaat taaatctgtc accagttcct ccaccttgat ttggaaggtt
     7501 ttgttgactt ggtgtttccc ccccttgtcc tggcaattga ttgttattgg ttggaaaatt
     7561 accatttgaa gacgcatcgc aatctcttga aaaaatagtt aatctttgag tagtgtagtc
     7621 gttttctggt attgattgat gattgcgata atgattagct ggcgtataaa tacctgtgat
     7681 aattgaatta aaccatcttg tttggaaatc atttacatta atgtagttaa ttgcacgtcc
     7741 aaaaccaatt gatgaaatat aattcaatgg aagaggcacc cctgtggcag cacttgcctc
     7801 tgctgcacaa ttggtatgat acgttgtcca cctttcgcct cctcttctga aatctccagc
     7861 gggcggattt ttactgtaat gataaaaatc ttctccttgt tctccaaacg gaggttgccc
     7921 acccctgcct ggctgggaag atatcccgct actcgccctt tccgccatgt cggcaaatag
     7981 catcacccca tcgtggggtc gcattccttt gatccaacca gcacaattca aggcaggaaa
     8041 gttacatgcg cattcaatat ttcccgccat gtgtgctggc attatgatag ctaatggccc
     8101 agtttcacca tgcgcatcgg cggcccagtc aaatgcagca tcgtgttcac gacacccctc
     8161 atgtgtatca catattctga aatgggtgta tcgccaaacc tctccactaa gtggatctgt
     8221 gtaaacaaaa aatggggaag atttacaagt gtcacaatca gggccacatg ctcctctaca
     8281 cattgagcca ctagctcttt gaggtcttct ttcataaact tctaagggac cttccaagcg
     8341 attaccagca ccataattcg cccatgtccc attgttttgt gaaggcagtt tatgaaaaac
     8401 ccttagaatg tcgcagatgg tttcgattag cggacaatca tccttaaacc cttccctgct
     8461 gattgctaag tcaaagtttt cgaagggtga gagtcctaaa tatcccccaa tgctcaatcc
     8521 aacaccgggg ctacttcctc cgggcacaat ggttagattt gacccaatcc ctaaggtggc
     8581 agctatttga ttatgccatt caaatggctg ccaatatatt ctacacaaat tttctcctaa
     8641 aagactgatt tggccgtatg cccccaaaaa ggcatcacct gccaatccca attcaagcgt
     8701 gctgaatcca cctacattaa ttgcccctcc cccatattcc aaccctcctc caattgtcca
     8761 ttttgttgtg ccaattcccc tcagcattac cgctacacct ccctctatac ttgtgagcgg
     8821 caccgaaatt ggaacaggta ctggtcccac cggcacaata gccattaatg ataattgccc
     8881 aataagccct ccccttgctt ctgctcctaa agaaccagca acagtagcac ttacgcttcc
     8941 actaattcca tagtgatgag aaaatgggtt gatgaccatt ctcactccct ctaaacaaat
     9001 tggtcctatt tgcgcttgaa attctggagc tattgtccca gtaacaccca cttctcccga
     9061 tataaataaa tctggcccta tcggaatggc aaatagggga accgtcttgt caccagaaat
     9121 tggttcaata tcaacctggt gagctaatgc tggtatcggg caaaatcttg gaagggtaat
     9181 aacaagtcca cttgaagtaa ttgagataac tcctccaaac ctatccacga atgct
//
LOCUS       DAORLF010000155         9200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4056_length_9200_cov_2.207217, whole genome shotgun sequence.
ACCESSION   DAORLF010000155 DAORLF010000000
VERSION     DAORLF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_9200_cov_2.207217"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK198_13055"
     CDS             complement(<1..555)
                     /locus_tag="PK198_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39711.1"
                     /translation="MRGSCSGTLTSVGCYDPEVGTMTGLNSNTTYYLRIFSYESGMFS
                     AFNLCLRVTPPAINDECTSAIAFPNIPGDGSCATVTSTTDGAVDKDVWFVFEAPADGA
                     EMYYEISPVIQSVAHRLNLYRGDCDNLSFTGRNYSSIAGKLEQLEAGETYYVRVFATS
                     STSPFSFCLRRLLSPANDECEGAIA"
     assembly_gap    592..662
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<663..>982)
                     /locus_tag="PK198_13060"
     CDS             complement(<663..>982)
                     /locus_tag="PK198_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39712.1"
                     /translation="GNPGISFQLYPSSCGTTPIGCFSSPSGIITGLAGSSVYYVRLYT
                     RTSNARAVFNLCLRAIVPPPNDQCAGAIALPSIPIDGTCMMAYVSTYGATGMQSTTCV
                     GSED"
     assembly_gap    983..1137
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1291..>1882)
                     /locus_tag="PK198_13065"
     CDS             complement(1291..>1882)
                     /locus_tag="PK198_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39713.1"
                     /translation="VTATATGTTNLICNGNQPLHDVWYRFTTPPGITSLHYTISTISG
                     ISDRVFQIFSGSSCADMTMLNCYDPETGFITGLSGNTTYFMRVFTKTNLLIQGGAFEI
                     CLRRPRINDNCPNAIAFPAIPADGSCATVTDGTTASTDSGLTACQGTADDDVWYSFIA
                     PAGVSALWYSFTPNRYDHAGVQRFLLTTSACGLLRQ"
     assembly_gap    1883..1894
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1895..2459)
                     /locus_tag="PK198_13070"
     CDS             complement(<1895..2459)
                     /locus_tag="PK198_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39714.1"
                     /translation="MKNLHHFFLMNAFAAAFLLFSTAGQATTCSNAIPITSFPVVGQP
                     LVCGSSNDLNSISVPGICGTGFNLYKEGKEALYTFTPSVTSTYIIQYFRGIFESAIWV
                     YSGCPASGGMCVGAVGSSEGYKELRVTLTAGVTYFIWFDVTPSLTSDSPCDFYGAFSI
                     TTPITNDECSSAISFPAIPTDANCSSVI"
     gene            complement(2696..3409)
                     /locus_tag="PK198_13075"
     CDS             complement(2696..3409)
                     /locus_tag="PK198_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39715.1"
                     /translation="MNFRFFIFAMLLMSAPLLNAQDFLDGATMFSHSKDAHFTLKTGE
                     TITAFVENVKRKKGLISAIEIKDAAGKKTVLSPDKVKHMYLPPTGYDKLLRATDISNN
                     VTKWDRDQSAHAQYIKGGYVYFEHTDVMVKKKKMSMMMQVINPGFADGIKVYFNPLAG
                     DTGGLAVGGLQVTGGDDRSCYFKKGDKAAYLIDKKNYSKEVKNLYGDCPDVAKAFGNK
                     FKWSEAAKHVAFYSENCSK"
     gene            complement(3463..6630)
                     /locus_tag="PK198_13080"
     CDS             complement(3463..6630)
                     /locus_tag="PK198_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF39716.1"
                     /translation="MNSKHFYLPMALFAAFLLFFIFPQDAAAQKKRPAPSVGSGPIAS
                     APTFDEALYQSVQWRSIGPFRGGRSAAVAGVPGKPNLFYFGSVGGGVWRTKDGGQTWE
                     NISDGYFGGSIGAVAVSEYDHNVIYVGGGEKTVRGNVSFGYGMWKSEDAGKTWKNMGL
                     NNSRHIPRIRIHPRNPDLVYAAVLGDLFKPTEERGVYRSKDGGKNWERILFANADAGA
                     VDLILDPANPRVLYASTWRIRRTPYSLESGGEGSALWKSTDGGDTWMEISKNKGFPKG
                     TLGIIGVSPSPVQQDRVWAIVEANEGGVYRSDDGGQSWQKLNEDRALRQRAWYYTRIY
                     ADTKDADIVYVLNVDYHKSTDGGRTFKSYEAPHGDHHDLWIAPEDNQRMVIGDDGGAQ
                     VSFDGGENWSTYHNQPTAQFYRVVTDNHFPYRIYGAQQDNSTVRILHRTDGGSITEQD
                     WEPTAGGESGHIAVDPLDNDIVYGGSYGGFLTRTNHKTNKTQAINVWPDNPIGHGAEG
                     MKYRFQWNFPIFFSPHNPKKLYTASNHLHSTTNGGQSWDIISPDLTRNDPAKLGPSGG
                     PITKDNTGVEYYCTIFAAIESPYEEGLLWTGSDDGLIHVSRDGGRNWQNVTPPGMPEW
                     MMVNSIDADPFNKGGVYIAGTRYKVGDYTPYLYKTEDYGRTWKLITKGIPAEHFTRVL
                     RADPARKGLLYAGTETGMYISFNDGANWQPFQLNLPIVPITDLTVKGHHLIAATQGRS
                     FWMIDDLGPIRQAQMDMPQKQMVLFQPADAYRMGGGGWGRPSKTAGANHPAGAMIHFY
                     IKDLPADSIVTKLGFYDAAGKLIREYASNAKERNEKLELKKGANMFVWNMLYPDAKSF
                     EGMVLWAASLRGPRAVPGEYKVRLSIGNQTQEQPLRILKDPRSEVSQADIQAQFDFVT
                     EVQKKVSEAHEVIIEIRDIRKQLNAYQERFKGKDEWKALADKAKAIDKAMTEVEEALY
                     QTKNRSGQDPLNFPIRLTNKLAGVASLSSMGDYPPTAQSIAVKNELTPLVDAELAKFR
                     QIKATELPAFNQMVKEKGVDAVIMK"
     gene            6773..7663
                     /locus_tag="PK198_13085"
     CDS             6773..7663
                     /locus_tag="PK198_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRF39717.1"
                     /translation="MHPERRAYIELHIAVLLFGVTAILGDLIQLSALVLVWWRVLLTS
                     LSLLLLVRLGSLLRRLPRAAILRYMGIGVIVALHWLAFYGAIKLANASIALVCMALTS
                     FFTSLLEPLIMRQRLRWYQLALGLLMVPGMALIVNSTELSMHNGIWTGIAAAFLAALF
                     STLNKRFVSQANPMEITLLELGSAWLFLSLVLPFYHRMLPQGLDRFWPAPSDWLWLAL
                     LAFLCTTLAYVLALRALRYLSAFVSNLTINLEPVYGIVLAWLLLGDNKELSPTFYAGV
                     ALILAVVFVYPTVERWVTKK"
     gene            complement(7771..8397)
                     /locus_tag="PK198_13090"
     CDS             complement(7771..8397)
                     /locus_tag="PK198_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39718.1"
                     /translation="MLMKVAERHGFRINTFTHILEGFKVADKMAKHGVGGSSFSDWWA
                     YKYEVYDAIPQNGAIMHEQGVTVAFNSDDAEMARRLNQEAAKAVLFGGVSEEDALKFV
                     TLNPAKLLRIDNRTGSLKAGKDADVVLWSDHPLSVYAKADMTFVDGIRFFDRQEDLKL
                     RSQMQSERHRIIQKMIKEKQGGGRTQPVMGRQSFKLYHCDDEEDEMMD"
     gene            complement(8391..>9200)
                     /locus_tag="PK198_13095"
     CDS             complement(8391..>9200)
                     /locus_tag="PK198_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF39719.1"
                     /translation="AWSVSPSAGTEAAAETKKSDKPAAKPEIGAANYPFGPYGWSALP
                     QAGAWLITNATVWTNEKEGILQNADVLMRAGKIVQVGKGIKAPDATVIDGTGKHVTAG
                     IIDEHSHIAASRVINEGSQVSSAEVRIGDIINSEDINIYRQLSGGVTTAQILHGSANP
                     IGGQSAIIKLRWGYTPEKMLFEGAPGFIKFALGENVKQSGWTGSDRYPQTRMGVEEVF
                     DDYFTRAREYGALKASGKPYRKDLDLETVGRFCKASASSPAIRIARARSTC"
BASE COUNT         1827 a         2393 c         2560 g         2182 t
ORIGIN      
        1 tgcaatggcg ccctcgcatt cgtcattggc cggcgatagt aatctgcgca ggcaaaaaga
       61 aaaaggagaa gtgcttgagg ttgcaaacac acgcacataa tacgtttctc cagcttcgag
      121 ttgctccagt tttccggcta tagacgagta gtttcttcct gtgaagctaa ggttgtcaca
      181 atcgccccga tacaaattca aacgatgggc gacgctctga ataacagggc tgatctcgta
      241 atacatttca gccccgtctg cgggagcttc aaaaacaaac catacgtctt tgtcaactgc
      301 gccatcggtg gtggacgtaa ccgtggcgca actcccgtcg ccgggaatat tgggaaatgc
      361 tatagcgctc gtacactcgt cattgatggc cgggggcgtc acgcgcaaac agagattgaa
      421 tgctgaaaac ataccgctct cataactgaa tatcctcagg taatatgtag tgttgctgtt
      481 caatccggtc atggtaccga cttcgggatc gtagcagccc actgaagtaa gcgtacccga
      541 acaggaacct cgcattaatt gaatgacacc gtcgtaagag ccacttagac gnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nngtcttctg atcccacaca ggtggtgctt tgcattccgg tggcgccgta agtacttaca
      721 taggccatca tgcaggtgcc gtcaatcggt atagacggca aggctatggc tcctgcgcac
      781 tgatcgttgg gcggcggcac aatagcccgc aggcacaggt tgaacactgc gcgggcgttt
      841 gatgtacgcg tgtataaacg cacataatac acactgctgc cagccagccc cgtgatgatg
      901 ccggaaggac tgctgaagca accaatagga gtagtgccgc aactgctggg gtaaagctga
      961 aaagaaatac cgggattgcc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga
     1141 atggcaggaa aagaaaaagc ccccgaacaa ttgtcgttgg ccacaggcgc ccgcaggcaa
     1201 aggttgtatt ctgatgcgac attggagtaa taggtatata cgcgcaaata ataggtgagg
     1261 cccggcgtca ggttggaaat ggctccgctt tcattgccgt aacagcccgc aggcagaagt
     1321 tgtgagcaag aaccgctgta cacctgcatg gtcatatcgg ttgggcgtaa acgaatacca
     1381 cagcgccgaa acgcccgccg gagcgatgaa tgaataccac acatcgtcgt cggcagtacc
     1441 ctggcaagct gtaagccccg aatcggtgga cgcagtcgtg ccgtcggtca cagtggcgca
     1501 ggaaccgtcg gcgggtatgg cgggaaatgc aatggcgttg gggcagttgt cattgatgcg
     1561 gggtcttcgc aggcagattt caaaagcccc gccttgaatc aacaaattgg tcttggtgaa
     1621 aaccctcata aaataagtgg tatttccgct caagcctgta atgaagccgg tttcggggtc
     1681 gtagcagttg agcatagtca tatcggcaca agaggaaccg ctgaatattt ggaacactct
     1741 gtcgctaata ccggaaatgg tactgatggt ataatgcaat gatgtgatgc cgggaggcgt
     1801 agtaaaacgg taccacacat cgtgtaaagg ctgatttcca ttgcaaatta gatttgtagt
     1861 tccggtggcc gtagctgtca cannnnnnnn nnnncgatca ccgaagagca gttcgcatct
     1921 gtagggatgg ccggaaagga tatagcggag gagcactcgt cgttggttat gggagttgtg
     1981 atactgaatg ctccgtaaaa gtcacaaggt gaatcgctgg tcaaagaggg ggttacatca
     2041 aaccaaatga aataggtgac tccggcagta agggtaaccc ttagttcttt gtacccttcc
     2101 gaagacccga cagcgcccac gcacatgccg ccggatgcgg ggcagcccga atacacccaa
     2161 atggcggatt cgaatatccc tctaaaatat tgaatgatgt atgtgctggt aacggaaggc
     2221 gtgaacgtgt agagcgcttc cttaccttct ttatatagat taaaccccgt accgcaaatt
     2281 ccgggcacgg atatactgtt gaggtcattg ctgctgccgc acaccaatgg ctgccccacc
     2341 accggaaagg aggtaatggg gatggcgttg ctgcaggtgg tagcttggcc cgcagtgctg
     2401 aaaagcaaaa aagccgctgc aaaagcattc atcagaaaga agtggtggag gtttttcatg
     2461 ctggtaggat tttttacatg aggtaggaga gaagagtttc tgtaaacagt agttaaaaat
     2521 ggagtcacac tgcgttgcgt cgctctcgac acgcttgggc aaaagtaaag tttttccggc
     2581 gttattttgt ctctgggaaa gcgctctttt tgggaaatat cccctcaaaa caaaaacgac
     2641 cgacccgaaa ttcatcgggc cggtcgtcgt tcagccttac gctgatgctg cgaaattact
     2701 tgctgcagtt ttcagaatag aaagccacat gcttggccgc ctccgaccac ttgaatttat
     2761 tgccgaaggc tttggccaca tccgggcagt cgccgtacag gtttttcacc tctttgctgt
     2821 agtttttctt atcaatcaaa taagcggctt tatcgccttt tttgaaataa caggaacggt
     2881 catcgccgcc cgttacttgc agtccgccta ctgccagccc gcctgtgtca ccagctaaag
     2941 gattgaaata cactttgatg ccgtcggcaa aacctgggtt gatgacctgc atcatcatgg
     3001 acattttttt cttttttacc attacatcgg tgtgttcaaa atacacatag ccgcccttga
     3061 tgtactgtgc atgtgcgctt tgatcgcggt cccatttcgt tacattgttg gaaatgtccg
     3121 tcgctctcaa aagtttgtcg tagcctgttg gcggcaggta catgtgcttc accttgtcgg
     3181 gcgacaaaac cgtctttttg ccggcggcat ctttgatctc aatggcgctg ataagccctt
     3241 ttttgcgctt cacgttttca acaaaggcgg tgatggtctc ccctgttttg agcgtaaaat
     3301 gggcgtcttt gctgtgagaa aacatagtgg cgccgtccaa aaagtcctga gcgttcagca
     3361 gtggtgcgct catcaagagc atagcgaaaa tgaaaaatct gaagttcata aaataaagga
     3421 tttaatgtgg tgaattaatg gcctgcaagg tacgaacaaa aatcacttca ttatcaccgc
     3481 atccacgccc ttttctttca ccatctggtt gaaagcgggc agttcggtcg ctttgatctg
     3541 gcggaacttg gccaattcgg catctaccaa cggcgtcagc tcgtttttca cggcaataga
     3601 ctgcgccgtc ggcggatagt cgcccatact gctgagactg gccacgcccg cgagcttgtt
     3661 ggtcaaacga atggggaagt tgagcgggtc ttgtccgctg cggtttttgg tttggtacaa
     3721 tgcctcctcc acctcggtca tggctttgtc tatggctttg gccttgtctg ccaacgcttt
     3781 ccactcgtct ttacctttga agcgctcctg gtaggcgttg agctgcttcc gtatgtcgcg
     3841 gatttcaatg atgacttcat gcgcttcgga gaccttcttt tgcacctcgg tcacgaagtc
     3901 gaactgcgcc tgtatgtcgg cctgactgac ctccgaacgc gggtctttga ggatgcgcaa
     3961 gggctgttcc tgtgtttgat tcccgatgct gagccgcact ttgtattcgc cgggcacggc
     4021 tctggggcca cgcaatgaag ccgcccataa taccatgccc tcgaagctct tggcatcggg
     4081 gtacagcata ttccagacaa acatattggc accttttttt aactccaatt tttcgttgcg
     4141 ctccttggcg ttgcttgcat actcgcggat gagcttgccg gcagcatcgt aaaaaccgag
     4201 cttcgttacg atcgaatcag cgggcaggtc tttgatgtaa aaatgaatca tggccccggc
     4261 cggatggttg gcgcccgcag ttttggaggg ccgtccccat ccgccgccgc ccatgcggta
     4321 agcatcggca ggttggaaca gcaccatctg tttctgaggc atgtccatct gtgcctgccg
     4381 gataggcccc agatcgtcta tcatccaaaa actgcggccc tgcgtagcgg ctatgaggtg
     4441 gtgacccttc acggtgaggt cggtgatggg cacgatgggc agattgagtt ggaagggctg
     4501 ccagttggcg ccgtcgttga aagaaatgta catgccggtt tcagtacccg catacagcaa
     4561 acctttgcgc gcagggtcgg cgcggagtac gcgggtgaaa tgctccgccg gaatgccttt
     4621 ggtgatgagt ttccaggtgc ggccgtagtc ctcggttttg tagagataag gcgtatagtc
     4681 gccgactttg tagcgggtgc cggctatgta aacaccgcct ttattgaaag gatcggcatc
     4741 aatgctgttg accatcatcc attcgggcat accgggcggc gtcacatttt gccagttgcg
     4801 cccgccgtcg cggctgacat gaatgaggcc gtcgtcggag ccggtccaga gcagcccttc
     4861 ctcatacgga gattctatgg cggcaaagat ggtgcagtag tactccactc ctgtgttgtc
     4921 tttggtgatg ggaccgccgg aagggccgag tttggcgggg tcgttgcggg tcaggtcggg
     4981 gctgatgatg tcccagcttt ggccgccgtt ggtggtggag tggaggtgat tggaggcggt
     5041 gtagagtttt ttgggattgt ggggcgagaa gaaaatgggg aagttccact ggaagcggta
     5101 cttcatacct tctgcgccgt gtccgatggg gttgtcgggc cacacgttga tggcttgcgt
     5161 tttgttggtt ttgtggttgg tgcgggtcag gaatccgcca tagctgccgc catacacgat
     5221 gtcgttgtcg agcgggtcca cggcgatgtg gccgctttcg ccgccggcag tgggctccca
     5281 gtcttgctcg gtgatggagc cgccgtcggt gcggtgcagg atgcgcacag tggagttgtc
     5341 ttgctgcgcg ccatagatgc ggtaggggaa gtggttatcg gtcacgacgc ggtaaaactg
     5401 cgcggtgggc tggttgtggt aggtgctcca gttttcgccg ccgtcgaagg atacctgtgc
     5461 gccgccgtcg tcgcctatga ccatgcgctg gttatcttcg ggcgctatcc agaggtcgtg
     5521 gtggtcgccg tggggtgctt cataagattt gaaggtgcgc ccgccgtcgg tggacttgtg
     5581 ataatccaca ttgagcacat acacaatgtc ggcgtctttg gtatcggcat agatgcgggt
     5641 gtagtaccag gcgcgctggc gcagggcgcg gtcttcgttg agcttttgcc agctttggcc
     5701 gccgtcgtcg gagcgataca cgccgccctc attggcctcc acgatggccc acacgcggtc
     5761 ttgctgcacg ggcgagggcg atacgccgat gatgccgagc gtgcctttgg gaaagccttt
     5821 gtttttagaa atctccatcc aggtatcgcc gccgtcggtg cttttccaca gggccgaacc
     5881 ctcgccgccg ctttctaagc tgtaaggagt acgacggatg cgccaggtgg aggcatagag
     5941 tacgcgcggg ttggccgggt cgaggatgag atcaacggcg ccggcatcgg cattggcaaa
     6001 caggatgcgc tcccagtttt tgccgccgtc tttgctgcga tacacgccgc gctcttcagt
     6061 gggtttgaag aggtcgccca gcacggctgc atacaccaga tcggggttgc gcggatggat
     6121 gcggatgcgc ggaatgtggc gcgagttgtt caagcccatg tttttccagg tcttgccggc
     6181 gtcttccgat ttccacatac cgtagccgaa ggagacattg ccgcgcacgg ttttttcgcc
     6241 gccgcccaca tagatgacgt tgtggtcgta ctccgacacc gccaccgcgc cgatggagcc
     6301 gccgaagtaa ccgtcggaga tgttttccca ggtctggccg ccgtctttgg tgcgccacac
     6361 gccgccgccc acgctgccga aatagaagag gttgggcttg cccggcacgc cggctacggc
     6421 cgccgaacga ccgccccgaa acggcccgat gctgcgccat tgcaccgact ggtacagcgc
     6481 ttcatcaaaa gtgggtgccg acgctatagg gccgctgcct actgaggggg ccgggcgttt
     6541 tttctgtgcc gccgcgtcct gtgggaaaat gaagaaaagg agaaatgccg caaaaagcgc
     6601 catcgggagg tagaaatgct tgctattcat tgatctgcaa tttttatcct tctttgacaa
     6661 atagtgtggg aacagattta gcggaaggca ggacgactgt aaggaggcat gattttcgct
     6721 aaatctgttc ctttaagcac actatttgtc aaagaatcaa aaaatcatcg ccatgcaccc
     6781 tgaacgcaga gcctatatag agttgcacat tgccgtgctg ttgtttggcg tcacggctat
     6841 tctcggcgac ctgattcagc tctcggcatt ggtgctcgta tggtggcgcg tgctgctcac
     6901 cagcctcagc ctcctgcttt tagtacgcct cggcagcctg cttcgtcgct tgccccgcgc
     6961 cgccattctg cgctacatgg gcatcggcgt cattgtggcc ctgcactggc tggcttttta
     7021 cggcgccatc aaattggcca acgcctccat cgctttggtg tgcatggccc tcacatcgtt
     7081 cttcacctcc ctgctcgaac ccctcatcat gcggcaacgc ctgcgttggt accagttggc
     7141 gctggggctg ctcatggtac ccggcatggc actcatcgtc aacagcacag aactgagcat
     7201 gcacaacggc atctggaccg gcatcgcggc ggcatttctg gcggccctgt tttctacgct
     7261 caacaagcgc ttcgtctcgc aggccaatcc catggaaatc actctgttgg aattgggcag
     7321 cgcctggctt ttcctcagcc tcgtactgcc gttttaccat cgcatgttgc cccaaggctt
     7381 ggaccgtttc tggccggcgc cttccgactg gctctggctg gcgctgctcg ctttcctctg
     7441 caccacgctg gcttatgtac tggcgctgag ggcattgcgc tacctctcgg ctttcgtttc
     7501 caacctcacc atcaacttag agccggtata tggcatcgtg ctggcctggc tgctgctggg
     7561 cgacaacaaa gaactctcgc ctacatttta cgcgggcgta gcgctcattt tggcggtggt
     7621 atttgtgtat ccgacggtag agcggtgggt tacaaaaaaa tgagtggatt caaaaacagg
     7681 gcgtgccgcc actccggttg aatccggcgg tggcgtcggg tgctccggtc cacttgctat
     7741 cgctgcgttc ccccttcgcc ccctcatgcg tcaatccatc atctcatctt cctcgtcatc
     7801 gcagtggtag agcttgaagc tctggcgccc catcacaggt tgcgttctgc cgcccccctg
     7861 cttttccttg atcatttttt gaataatgcg gtggcgctcg ctctgcatct gactgcgaag
     7921 cttgaggtct tcctgccggt cgaagaaacg gatgccgtcc acgaaagtca tgtccgcttt
     7981 ggcatatacc gagagcgggt gatccgacca cagcaccacg tcggcgtctt taccggcttt
     8041 gaggctgccc gtgcggttgt caatgcgcag gagcttggcc ggattgaggg ttacaaattt
     8101 gagcgcatct tcttccgaaa cgccgccgaa cagcaccgcc ttggcagcct cctgattgag
     8161 acggcgcgcc atttcggcat cgtcggagtt gaaagccacc gtcacgccct gttcgtgcat
     8221 gatggcgccg ttttgcggaa tggcgtcata cacctcgtat ttgtaagccc accagtcgga
     8281 gaaactggag ccgcccacgc cgtgtttggc cattttgtcg gccactttga agccctccag
     8341 aatgtgcgtg aaagtattga tgcggaagcc gtgccgctcg gccactttca tcagcatgtt
     8401 gatctcgctc tggcgatacg aatggcaggt gatgaagcgc ttgctttgca aaatctccca
     8461 acggtctcca gatcaaggtc tttgcgatag ggtttgcccg aagccttcag cgctccgtac
     8521 tcgcgggcgc gggtgaagta gtcgtcaaac acctcctcta cgcccatgcg ggtttgcgga
     8581 tagcggtcgc tgcccgtcca gcccgattgt ttcacgtttt cgcccagggc aaatttgatg
     8641 aagcccggcg caccctcaaa cagcattttt tcgggcgtat aaccccagcg cagtttgatg
     8701 atggccgact gcccgccgat ggggttggcc gagccgtgca gaatttgagc ggtagtgacg
     8761 ccccccgaca actgcctgta aatgttgatg tcttcggagt tgatgatgtc gccgatgcgc
     8821 acttccgcgc tgctcacctg cgagccttcg ttgatgacgc gcgaggctgc tatgtgcgag
     8881 tgctcgtcta tgatgcccgc tgtgacgtgc ttgcccgtgc cgtcaatgac ggttgcgtcc
     8941 ggcgctttga tgcccttgcc tacctgcaca atcttgccgg cgcgcatcag cacgtctgca
     9001 ttttgcagga tgccttcttt ttcgttggtc cacaccgttg cgttggtgat gagccaggcg
     9061 ccggcctgcg gcaatgccga ccagccataa gggccaaacg gatagttggc agcgccgatt
     9121 tcgggcttgg cagcgggttt gtcgcttttt ttcgtctccg cggcagcttc agttccggca
     9181 gatggcgaga ccgaccaagc
//
LOCUS       DAORLF010000156         9186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4065_length_9186_cov_2.114117, whole genome shotgun sequence.
ACCESSION   DAORLF010000156 DAORLF010000000
VERSION     DAORLF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4065_length_9186_cov_2.114117"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK198_13100"
     CDS             complement(<1..296)
                     /locus_tag="PK198_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39720.1"
                     /translation="MRIFYTLFSLLLLSHIATAQLGSLHTYSFLQLSPSARVTGLGGN
                     LITVVDDDAALAYHNPSLLNAAMHQQLSFNHAFLSNGIQHGYASFAHHADAWKTT"
     gene            457..1134
                     /gene="ung"
                     /locus_tag="PK198_13105"
     CDS             457..1134
                     /gene="ung"
                     /locus_tag="PK198_13105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRF39721.1"
                     /translation="MSNVKIEAGWKAALAGEFEQPYFEAIASFLRSEKAAGKVVYPPG
                     GLIFNAFDSTPFDQVKVVILGQDPYHNPGEAMGLCFSVPRGVKVPPSLVNIYKELQAD
                     LGIPATGHGDLSSWTRQGVFLLNAMLTVERNRPQSHQNIGWQTFTDAVIRTLSERREG
                     LVFMLWGNFARKKKTLIDTERHLVLESAHPSPLAGNAFSGCRHFSKANAYLESKGVVP
                     VDWRVEG"
     gene            complement(1306..1566)
                     /locus_tag="PK198_13110"
     CDS             complement(1306..1566)
                     /locus_tag="PK198_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39722.1"
                     /translation="MRTALSRIIGSVLLIAALSVVASPTVSAQCPMCRMSAESNLENG
                     GTEGKGLNTGILYLLAMPYLLVGGIGYIWWRNRRRETVEAEI"
     gene            complement(1580..2161)
                     /locus_tag="PK198_13115"
     CDS             complement(1580..2161)
                     /locus_tag="PK198_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRF39723.1"
                     /translation="MESVNLSIARKLNTYAWIFTAVVLLLVGAMRRIKIPLPEGWDFS
                     FLPPFHAGVNALTAVVLLLALYFIKQKNVAAHRRAIYVAMSLSVLFLLSYVAYHFTTP
                     ETIYGDVDGNGRLDAGELAAVGSARTAYLVLLLSHIVLAAVSLPLILFTFIRGFTDQV
                     ERHRRMARWVFPLWLYVAVTGPVCYWMLKPFYP"
     gene            complement(2168..2773)
                     /locus_tag="PK198_13120"
     CDS             complement(2168..2773)
                     /locus_tag="PK198_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39724.1"
                     /translation="MNRKNITQTLVLLLLLAGLPLGSWYYLNKGYNYRKTRMDQLQNL
                     SSLPAVELPDHRDSLIRLDDFRGKALVLADIEATDAAALEALRNIGDQFAESGAVSIA
                     VLAPDADATAMQAPFQAATQERPGLFHVLKNGSEQAAQLRKNMSLSGTANVALADTSL
                     MVRQVYDLSNAQDIRRLVDHLVIVIPSKRSPKPVLKRPTEK"
     gene            complement(2845..>4102)
                     /locus_tag="PK198_13125"
     CDS             complement(2845..>4102)
                     /locus_tag="PK198_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRF39725.1"
                     /translation="RGEAFAEARPSQQKVWVGQQLILDYLIYLSDPDWTVSIAKESDY
                     DGMVPVPWGNTTQGKERIKGTEYNTLTERISLYPQQSGKHTIGPLHLDVMEWQRDQFS
                     PFGHSSKRMSIATEPAEIEVLPLPAGAPADFTGAVGRFVMQCEWPKTAGSTDETFVLK
                     MTIAGNGDMKRVEAPALRLPDGLQGYPPKTSEAPAPKSYEQQTAQRDIEYLITAKQPG
                     DYTIQPQFVYFDTDSARYVTLSAGPFSLKVSPGNNRADNEQEASSSENDAPRKRAAWL
                     LPTLAAAAIFVSAALVFFLLRRRKKMPAHEQSSPELHPRERLSLAAKYLHTGNTEAFY
                     TEISAAMYEFCGGQLGLPPSEWTRRQIRQAMSEHGYEAALMDGVERILQAAERARYAR
                     MDMSASAAETYKMAVELMEKMSIRSS"
     assembly_gap    4103..4114
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4115..4501)
                     /locus_tag="PK198_13130"
     CDS             complement(<4115..4501)
                     /locus_tag="PK198_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRF39726.1"
                     /translation="MRCFLLRSFVFTFCLGAALSVRAQSAPRFEASASVNKVNLNSSF
                     EVSFTLFDAEGTRFQAPDFSDFDIDYGPATRINASFVNGKASSESAYVYSLRPRRTGT
                     LTIGSASIQAGNTTLRTKPIQIQVQPG"
     gene            4634..5152
                     /locus_tag="PK198_13135"
     CDS             4634..5152
                     /locus_tag="PK198_13135"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRF39727.1"
                     /translation="MSTEAAEREMIVSAIVAVARNGVIGHNNQIPWYLPADLQYFKRT
                     TLNHHIIMGRRSFLSIGRALPHRTNIVITRDPYFAATGCLVAYSVEMALEMAFDNEET
                     EAFIIGGGEIYRESMPHWDRIYLTEVDVELPGDVFFPQPDWQDWQLRSSAQQQPDEKN
                     EYAYCFKVFEKI"
     gene            5162..6217
                     /locus_tag="PK198_13140"
     CDS             5162..6217
                     /locus_tag="PK198_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRF39728.1"
                     /translation="MPVRPYIRPIAFWMMLLAISAAFFGMIGPFLMALFWAAMLAMIF
                     SRPYRLLRLRLKGKENLAAFITTIFIVLLVVLPGFFIVLALVNESVGVYNKINSGEWD
                     LTGIVDYIQTETPRLESLLREVGMTPERLKADLANFAVNAARTVADRLLSYTQNAIAF
                     TVQFFLMLYILFFFLRDGRQIVHSVMNALPLGNRWERILIERFSGVARATLKGTLIVA
                     MVQGTIGGLAFAILGIEGAMFWGVIMTILSILPVGGSGLIWGPAAIMLATQGMWGKAV
                     VLALVGALGIGLIDNVLRPILVGRDTKMPDFLVLLATLGGLGWFGLSGFVLGPVIAAL
                     FLTFWEAAGREYGGAET"
     gene            complement(6242..>7356)
                     /locus_tag="PK198_13145"
     CDS             complement(6242..>7356)
                     /locus_tag="PK198_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRF39729.1"
                     /translation="RFLLDNNVAEEVSKPFKKGSGLYMVASRYLLPASLILLCLGIDF
                     KGIVRLGPKALIMFLTATAGVILGGPIALLAVTTVAPDLISVPREDLWKGLSTIAGSW
                     IGGGANQTAMREIFNVSQNLFGTMVVVDVVVANIWMAVLLYGAGIRERIDRAFKADTS
                     AIDDLVQRVRAFRAGVERIPNNTDMFKLLAAAFGGVALSHWGAQLITKALSPYQEQLV
                     AMRLESINSSFFWIVVISTTFGLLLSFTKARNLEGAGASRWGTVFLYVLVATIGMQMD
                     LGAVFKNLGLFAVGLVWMLTHAVTLLVVAKLIRAPFFFAAVGSQANIGGAASAPVVAT
                     AFDPVLAPVGVLLAVLGYALGTYGALVCAFLMEMAS"
     assembly_gap    7357..7366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7367..7633)
                     /locus_tag="PK198_13150"
     CDS             complement(<7367..7633)
                     /locus_tag="PK198_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39730.1"
                     /translation="MKPFFSNDAIVLGLLFTVLALVFITSGSKHKAWQGFYRIFPPLL
                     MCYFLPALLHWPLGIIANDWYEAGLWDELRKLGLNPPSAASFSEM"
     assembly_gap    7747..7782
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7783..8216
                     /gene="lspA"
                     /locus_tag="PK198_13155"
     CDS             <7783..8216
                     /gene="lspA"
                     /locus_tag="PK198_13155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRF39731.1"
                     /translation="WTKLLAIEHLKDQPMRDVAGRFFQLVFVENTGAFLSFGAGFPDA
                     VKLIVLTIMPSVLLGALLLYTLFSTQLNTWQVIAFSLISGGGISNLFDRITEGKVVDF
                     MIMGIGALRTGVFNTADVCIMIGLFMMLPYMLGYGQPKNTA"
     gene            8337..>9186
                     /locus_tag="PK198_13160"
     CDS             8337..>9186
                     /locus_tag="PK198_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF39732.1"
                     /translation="MKLTPPVILAIMLGYFLVLIAVSYFTSRNKSTNLNFFLAGRQAP
                     WPLVAFGMIGSTISGITFISVPGAVGGMGANEAFSYFQVVLGYLLGYVVIAFVLLPLY
                     YRLHLTSIYGYLEQRFGFFSYKTGAAYFLLSRSIGSALRLFLVAVVVYNFVLKPMEVP
                     FALSVFLILALIWTYTFKGGIKTIIVTDALQTAFLLGAVILTVIFISQSLDLGLGGMM
                     EMVSNSKYSKTFYFEGGWSDPNNFFKQFFSGALITITMTGLDQDMMQKNLSCRNLGDA
                     QKNMLSF"
BASE COUNT         2017 a         2390 c         2635 g         2086 t
ORIGIN      
        1 gtggttttcc aggcatcggc gtggtgggca aaggaggcgt agccgtgttg aataccattg
       61 ctgagaaagg cgtggttgaa cgacaattgc tggtgcatgg cggcattgag caggctggga
      121 ttgtggtagg ccagcgctgc gtcgtcgtcc actacggtga tgagattgcc gcccagcccg
      181 gtgacgcgcg ccgagggaga aagttgtaaa aatgagtaag tgtgcaagct gcccaactgg
      241 gcggtagcga tgtgtgagag cagcagcagg ctgaaaagtg tatagaatat tctcatacgg
      301 tcaacaatga ttgggagagt ggaaacattc aggatgcaaa caaagcgaat ggtttttaaa
      361 ttgttcggga aaggataaat aattcgcagt tgtgtcgggt gaaaggcggt ctattctttg
      421 ctacttttgc cgaaaggcac attaattcga gacaagatga gcaacgtaaa aatagaagcc
      481 ggctggaagg ccgcgctggc cggggagttt gaacagcctt attttgaggc cattgcgtcg
      541 tttttgcgca gcgagaaggc cgccggcaag gtcgtttatc ctccgggagg cttgattttc
      601 aacgcattcg actccacgcc tttcgatcaa gtgaaggtgg tgatactggg gcaagacccc
      661 taccacaatc ccggcgaggc gatggggctg tgcttctcgg tgccgcgcgg cgtgaaagtg
      721 ccgccttcgc tcgtcaatat ttacaaagaa ttgcaggccg atctgggcat tcccgcaacg
      781 gggcacggcg acctgagcag ttggacccgg cagggtgtgt ttttgctcaa cgccatgctg
      841 acggtagagc gcaaccggcc tcaaagccac caaaatatag gctggcaaac ctttaccgat
      901 gcggtgatcc gcactttgtc ggagcggcgc gaaggattgg tgtttatgct gtggggcaat
      961 tttgcgcgca agaagaaaac attgattgac acggaacggc acttggtgct ggaatcggcg
     1021 cacccctcgc cgttggcggg caatgccttt tcgggctgcc ggcatttctc gaaagcgaat
     1081 gcgtatttgg aatcaaaggg ggtggtgccg gtggattgga gggtggaggg ttgagcggcg
     1141 cccgccttgc gcgcgaggga tagcagcgga atggcggcgg cgtagatacg ttgcgcgcaa
     1201 cgtttccgaa gagaacgccg ccatgcagcg gatagcccgg ccccaccgcc tgtttcgatc
     1261 ggatggcaag agatgagact gagtggggcg cgccctgaaa caatgctata tctccgcctc
     1321 taccgtttcc cgacggcgat tgcgccacca gatgtacccg atgccgccca ccagcaggta
     1381 gggcatggcc agcagataga ggatgccggt attgagacct ttgccttcgg tgccgccgtt
     1441 ttcgagattg ctttcggccg acatgcggca catggggcat tgggctgaaa ccgtaggcga
     1501 ggcaacgacg ctgagtgcgg ctatgagcaa aacggaaccg atgatgcggg agagtgcggt
     1561 gcgcatattg tattgatttt taaggataaa acggcttcag catccaatag cataccgggc
     1621 cggtgacggc tacatacaac cacagtggga acacccagcg ggccatgcgg cggtggcgtt
     1681 cgacctgatc ggtgaatccg cggataaagg taaacaatat gagcggaagg ctgacggctg
     1741 ccaatacaat gtggctgagc aacaaaacga ggtacgccgt gcgcgctgaa ccaacggcgg
     1801 ccaattcgcc tgcatccaag cgaccgttgc cgtccacatc gccgtaaatg gtttcgggcg
     1861 tggtgaagtg gtaggccaca taagacagca ggaacagcac cgacaggctc atggccacat
     1921 agatggcgcg acgatgggcg gccacgtttt tctgtttgat gaagtacaag gccagcagca
     1981 gcacaacggc ggtgagggca ttgacgccgg cgtgaaacgg aggaaggaag gagaagtccc
     2041 agccttcggg cagggggatt ttgatgcgcc gcatggcgcc cacgagcagc agcaccacgg
     2101 cggtaaaaat ccaggcgtaa gtgttgagtt tgcgggcgat agagaggttg acggattcca
     2161 ttggatgtta tttttcggtg ggtcttttta atacgggttt gggactgcgc ttggacggaa
     2221 tgacaatgac caaatgatct acgaggcggc gaatgtcttg cgcgttgctc aggtcataga
     2281 cctgccgcac catgagcgag gtgtcggcga gtgcaacgtt ggcggtgccg ctcaaagaca
     2341 tgtttttgcg aagctgtgct gcctgttccg agccgttttt gagcacatga aaaaggccgg
     2401 gccgctcctg agttgccgcc tgaaacgggg cctgcatggc agtggcatca gcgtcggggg
     2461 ccagtacggc aatactgacg gcgccgcttt cggcaaattg gtcgccaatg ttgcggagcg
     2521 cttccaaagc ggcggcgtca gtggcttcaa tgtcggcaag cactaaagct ttgccacgaa
     2581 aatcatccag acgaatgagc gaatcgcggt gatcgggcag ttctacagca ggcaggctgc
     2641 tcaggttttg cagttggtcc atgcgtgtct tgcggtagtt gtaccctttg ttaaggtagt
     2701 accaggaccc cagcggaagc cccgccagca agagcagcag gacgagtgtt tgagtgatgt
     2761 ttttgcgatt caagtgtgca gtatttttta gctcgaacaa ccaacggcgg cacaataggt
     2821 tgtcgaaaaa gtgccatttg tgcgtcagga cgagcgaatt gacattttct ccatcagctc
     2881 caccgccatt ttgtacgttt cggcagccga ggccgacatg tccattcggg cgtatcgggc
     2941 gcgctcggcg gcctgtagga tgcgctctac cccatccatc aacgcagcct cgtatccgtg
     3001 ttccgacata gcctgccgga tctgccggcg tgtccattcc gaagggggta ggcccaattg
     3061 accgccgcaa aattcgtaca tcgccgccga tatttcggta taaaatgcct ccgtatttcc
     3121 ggtgtgcagg tatttggccg ccaaagacag gcgctctcgc gggtgcagtt ccggtgagga
     3181 ctgctcgtgg gcgggcattt ttttgcgccg gcgaagcaag aaaaaaacaa gcgcagcaga
     3241 aacgaaaatc gcggcagcgg ccaacgtcgg caagagccat gcagcccgtt ttcggggagc
     3301 atcattttca gatgaggagg cttcctgctc gttatccgcc cggttgttgc cgggacttac
     3361 cttcagcgaa aacgggcctg cactcagcgt gacataccgg gcgctgtcgg tatcgaaata
     3421 gacgaactga ggctgtatgg tataatcacc gggctgcttg gccgtgatga ggtactcgat
     3481 gtctcgctgc gcggtttgtt gttcgtaaga cttaggcgcg ggggcctcgc tcgttttggg
     3541 cggatacccc tgcaagccat cgggcaaacg gagagcgggc gcttccaccc gtttcatgtc
     3601 gccgtttccg gcaatggtca ttttcaaaac aaaagtctcg tccgtgctgc cggcggtttt
     3661 gggccattcg cattgcatca caaagcgccc cactgccccg gtaaagtcgg cgggcgcgcc
     3721 ggcgggcagc ggcaaaacct ctatttcggc gggttcggtg gcaatgctca tgcgcttgga
     3781 ggagtggcca aaggggctga attgatcgcg ctgccattcc atcacatcca agtggagcgg
     3841 cccgatggtg tgcttccccg attgctgcgg gtacaggctg atgcgctcgg tgagggtgtt
     3901 gtactccgtg cctttgatgc gctctttgcc ctgcgtggta ttgccccagg gaacaggtac
     3961 catgccgtcg tagtcggatt ccttcgcgat gctcaccgtc caatcggggt cggagagata
     4021 gatgagatag tcgagtatca attgctggcc cacccatact ttttgctgag aaggtcgggc
     4081 ttcagcaaag gcttctccgc ggnnnnnnnn nnnngccggg ctgcacctga atttggatcg
     4141 gcttggtgcg cagcgttgtg ttgcctgcct gaatagaggc gctgccaatg gtgagcgtgc
     4201 cggtgcgacg ggggcgcagc gaatacacat aggcgctttc gctggatgcc ttgccgttga
     4261 caaaagaggc attgatgcgg gtggcggggc cgtaatctat gtcaaaatcg gagaagtcgg
     4321 gcgcctgaaa acgcgtgcct tccgcgtcga acagagtgaa cgacacctca aaactgctgt
     4381 tcagattcac cttgttcacg cttgcagaag cctcaaagcg cggcgccgat tgtgcccgga
     4441 cactcagggc ggccccaagg caaaacgtaa aaacgaatga acgaagcaaa aaacagcgca
     4501 tggcatacct gatgtggaaa gctcgaaatc tgggcaaagt taggaggtaa cttcgaagtt
     4561 acctcctaac ttcttctgta gttttttatt cacttgggtt tgcgctacaa aatatcctct
     4621 ccatctttgc gcaatgagta cagaagcagc agaacgcgaa atgattgttt cggccattgt
     4681 ggcggtggcc cgcaacggcg tcatcgggca caacaaccaa atcccctggt acttgcccgc
     4741 cgatctgcaa tatttcaaac gcaccacgct caaccatcac atcatcatgg ggcggcgcag
     4801 ttttttgtcc atcggcaggg cgctgccgca ccgcaccaac atcgtcatca cccgcgatcc
     4861 gtactttgcc gccacgggct gcttggtagc ttacagcgta gaaatggcgc tggaaatggc
     4921 gttcgacaac gaagaaaccg aggcattcat catcggcggc ggcgaaatct atcgcgaaag
     4981 tatgccgcat tgggaccgca tttacctcac cgaggtggat gtggaactgc ccggagatgt
     5041 gttcttcccg caacccgact ggcaggactg gcagttgcgg tcttcagcgc agcagcagcc
     5101 ggatgagaaa aatgagtatg cgtattgttt caaggttttt gaaaagattt aacatcacca
     5161 catgcctgta cgtccctaca tccgacccat tgctttctgg atgatgctcc tggccatctc
     5221 cgctgccttt ttcggcatga tcggcccttt tctcatggcg ctcttttggg ccgctatgct
     5281 ggccatgatc ttcagtcggc cttatcgttt gttgcggctt cggctcaaag gcaaggagaa
     5341 tctggccgcc ttcatcacta ctatttttat agtattgctg gtggttttgc ccggcttttt
     5401 catcgtgctg gccttggtca acgagagtgt gggcgtttac aacaaaatca attccggcga
     5461 gtgggatctc accggcatag ttgattatat acaaaccgaa acgccccgcc tcgaaagcct
     5521 cctccgcgaa gtaggcatga cgccggaacg cttgaaagcc gacttggcca acttcgccgt
     5581 caatgccgcc cgcaccgtag ccgaccgcct gctgagctat acccaaaacg ccattgcgtt
     5641 cacggtgcag ttcttcctca tgctttatat tctgttcttt ttcctccgcg acggccgtca
     5701 gattgtacat tccgttatga acgccctgcc tttgggcaat cgctgggaac gcatcctcat
     5761 cgagcgtttt tcgggcgtag cgcgcgccac cctcaaaggg acgcttatcg tggctatggt
     5821 acagggaacc atcggcgggc tggcgtttgc catcctcggc atagagggcg ccatgttctg
     5881 gggcgttatt atgaccattc tttccatcct gcccgtagga ggcagcggcc tcatatgggg
     5941 gcctgccgcc atcatgctgg ccacgcaggg tatgtggggc aaagccgtcg tgctggcctt
     6001 ggtgggggct ttgggtattg gtttgataga caatgtattg cgccccattc tcgtagggcg
     6061 cgacaccaaa atgcccgact tcctggtact gttggccacc ttgggcggcc tgggttggtt
     6121 cggcctgagc ggctttgtac tcggcccggt catagcggcg ctgttcctca cgttttggga
     6181 ggctgcgggg agagaatacg gcggagcaga gacgtagtcc tgcattttgt ataaaaatcc
     6241 gtcaggaagc catttccatc aaaaacgcgc aaaccaaggc gccgtaagta cccaatgcat
     6301 agcccaatac ggccagcagt acgcccaccg gcgccagcac cggatcgaac gcggtggcta
     6361 ctaccggcgc agatgccgca ccacctatgt tggcctgact gcccactgcg gcgaaaaaga
     6421 agggtgcccg tatgagtttg gccactacga gcaaagtcac ggcgtgcgtg agcatccaaa
     6481 ccaaaccgac cgcgaaaagc cccaggtttt tgaacaccgc gcccaggtcc atttgcatgc
     6541 cgatggtagc taccagcaca tacaaaaata cggtgcccca acgcgaggcg cctgcgcctt
     6601 ccagattgcg cgccttggtg aaagacagca gcaaaccgaa tgtggtggaa atgaccacga
     6661 tccagaaaaa cgaagaattg atggactcca agcgcatggc caccaattgc tcctgatatg
     6721 gactcaaggc tttggtaatg agctgcgcgc cccaatgcga caaggctacg ccgccgaaag
     6781 ctgcggccaa cagcttgaac atatctgtat tgttggggat gcgctccacg ccggcccgga
     6841 atgcgcgcac ccgctgcacc aaatcgtcaa tagcgctggt atcggccttg aaagcccggt
     6901 ctatgcgctc ccgaatgccc gcgccgtaca gcagcactgc catccagata ttggccacca
     6961 ccacatccac caccaccata gtgccgaaga ggttttggct cacgttgaaa atttcccgca
     7021 tggccgtctg gttggcgccg ccgcctatcc aggaaccggc gatggtggaa agccctttcc
     7081 agaggtcttc gcggggcacg ctgatgagat cgggcgctac cgtggtgact gccagcaaag
     7141 caatggggcc gcccaaaatg acgcccgccg tagcggtgag gaacatgatg agggccttgg
     7201 ggcctaatcg cacaatgcct ttgaagtcaa tgccgagaca cagcaaaata aggctggccg
     7261 gcagcagata ccgggaagcg accatgtaca atccagagcc ttttttgaag ggtttgctca
     7321 cttcttcggc cacgttgttg tccaacagaa agcgctnnnn nnnnnncatt tctgaaaaag
     7381 aagcggcgga gggcgggttc aggcccaatt tgcgcaactc gtcccagaga ccggcttcat
     7441 accaatcgtt ggcaatgatg cccaggggcc aatgcagcag cgccggcaga aaatagcaca
     7501 tcagcagcgg cggaaaaatg cggtaaaacc cctgccaggc tttgtgtttg ctgcccgatg
     7561 tgatgaaaac caaagccaac actgtgaaca aaagtccgag tacaatggcg tcgttgctga
     7621 aaaaaggttt catgcttttg atggtgtttt ttgacagaat cagggctaaa gatacccgtt
     7681 tgccggaaat tgtataattt tccgccccat tcgcacccga tatgaaaaga tacctgttgg
     7741 tcattgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctggacg aaactcttag
     7801 ccatcgagca cctgaaagac cagcccatgc gggatgttgc cggtcgtttt tttcagttgg
     7861 tttttgtcga aaacaccggt gcgtttctca gcttcggcgc cggttttccc gatgcagtca
     7921 agctgattgt gctcaccatt atgccttccg tattgttggg cgctttgttg ttatacacct
     7981 tgttcagcac gcaactcaat acctggcagg tcatagcttt cagtctcatc agcggcggcg
     8041 gcatcagcaa tctgttcgac cgcattaccg agggcaaagt agtggatttt atgatcatgg
     8101 gcatcggggc gctgcgaacg ggcgttttca ataccgccga tgtgtgcatt atgatcggcc
     8161 tgtttatgat gctgccatac atgctgggct acggccaacc taaaaacact gcataatcgc
     8221 gccttattaa atgtgctaat ttgacaatat gctaatgtgc tgattgccaa tcgagagcga
     8281 ccaaatttgt gtcaattgat ggaagtcaag cacttaggca aataatacca accgatatga
     8341 aactcacgcc acctgtcatc ctcgccatta tgctcggcta cttccttgtg ttgatagccg
     8401 tgtcttattt taccagcagg aacaaaagca ccaacctgaa cttctttctc gcaggcaggc
     8461 aggcgccctg gcctttggtg gcttttggta tgataggctc caccatttcc ggcattacat
     8521 ttatatctgt gccgggcgcg gtgggcggca tgggggccaa tgaggcattt tcctactttc
     8581 aggtggtatt ggggtatttg ttggggtatg tggtcattgc ttttgtattg ttgccattgt
     8641 attatcgctt gcatcttact tccatctacg gttatttgga gcagcgattc ggtttttttt
     8701 cttacaaaac cggagccgcg tattttctgc tctcgcgcag catcggttcg gcactgaggt
     8761 tgtttttggt ggctgtggtg gtttacaatt tcgtactaaa acctatggag gtaccatttg
     8821 cgctctccgt tttcctcatt ttagccctca tctggactta tacgtttaaa ggcggcatca
     8881 aaaccatcat cgtcacggat gcgctgcaaa cggccttttt actcggcgcc gtcatactga
     8941 ccgtcatatt tatatctcag tccttagacc ttggcctcgg cgggatgatg gagatggtgt
     9001 cgaacagcaa gtattccaaa accttttatt ttgaaggcgg ctggagcgac cccaacaact
     9061 tcttcaagca gtttttcagc ggggcgctca tcaccatcac catgacgggc ttagaccagg
     9121 acatgatgca aaaaaacctg agctgccgca atttgggcga tgcgcaaaaa aatatgctct
     9181 cattca
//
LOCUS       DAORLF010000157         9185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4066_length_9185_cov_2.368018, whole genome shotgun sequence.
ACCESSION   DAORLF010000157 DAORLF010000000
VERSION     DAORLF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_9185_cov_2.368018"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>354)
                     /locus_tag="PK198_13165"
     CDS             complement(<1..>354)
                     /locus_tag="PK198_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRF39733.1"
                     /translation="VRVVSGPGNKDYGVISVLLQAFYEGKYLFSVDKSAFQPPPQVQS
                     GVIRLLRKPQPSLPCDEALFRSVVKITFGQRRKMLRNTLRPFFSQQPEALQDDFFLKR
                     PEQLSVQDFIELTLRV"
     assembly_gap    355..500
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<501..764)
                     /locus_tag="PK198_13170"
     CDS             complement(<501..764)
                     /locus_tag="PK198_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRF39734.1"
                     /translation="MKAKKSYGQHFLHRDDIAARIAASLTQPYERVLEVGPGKGMLTK
                     HLGAPGRAVVAVEADRDMVEYLDARPPLEGLRVISADFLQVDLS"
     gene            947..>1764
                     /locus_tag="PK198_13175"
     CDS             947..>1764
                     /locus_tag="PK198_13175"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRF39735.1"
                     /translation="MELPKIGITVGDINGIGLEVIIKTLSEKKILDYCVPVIYGSSKV
                     VSYHKNIVGDDFQFVPIRSAERITPERVNVVNCWNEVVNISLGKVVLEGGQYAFKALE
                     MAVGELKAGFIDALVTAPISKEAMQMAGFPFPGHTEYIAKELSAPESLMLMVSDALRV
                     GLATNHLPIREVAGAITKEVLLKKIQIFHETLQIDFDKDRPTIAVMGLNPHAGENGML
                     GEEEEKIIRPAIVECKKKGIVVNGPFSADGFFGSGQYHKYDGILAMYHDQGLIP"
     assembly_gap    1765..1918
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2035..2418
                     /locus_tag="PK198_13180"
     CDS             2035..2418
                     /locus_tag="PK198_13180"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRF39736.1"
                     /translation="MEDKRKLRLLAAVVMTVLLVDQSVKFWIKTNMSIGEEMYPLGVK
                     WAVIHFVENPGMAFGLTFGGEYGKLALSLFRIVAVVWFLFALAVSRARGGHNAGGLWP
                     HSGGRAGQYHRQRFLRPDFFGIHLS"
     gene            2309..2794
                     /locus_tag="PK198_13185"
     CDS             2309..2794
                     /locus_tag="PK198_13185"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRF39737.1"
                     /translation="MAGIMLAGFGLILAGALGNIIDSAFYDLIFSESTYHSDPAILFP
                     DEGGYAGFLHGKVVDMLYFPIAEGYYPDWFPFWSGEPYQFFRPVFNVADMAISCGVTL
                     VLILHVFFSKMIHPMPAPALVTHATNAETAVIEDTSTEENDPADSSDPDEASQDPVIP
                     A"
     gene            complement(2911..6039)
                     /locus_tag="PK198_13190"
     CDS             complement(2911..6039)
                     /locus_tag="PK198_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39738.1"
                     /translation="MNKKFRLFAFHFWAWICLLSAGVLQAQQQNTKILFPHPAGFSQS
                     SGQFVRATNDGGYILLGSATASSSNSYVQLPRAIRLDAGLQVVWDETYLPAPQPYIIA
                     EASSAPLELPDGSFVFAVQDSTSDFDLVRISGAGEFLWGIDLPGFSSATYPLGVLPNG
                     HIFVTHCRFQFPGNSCGTLEIDLDGHEVQYQPIISLPNLAHSDVFPNGDALTLHFSQG
                     KAVFSRIAQNNQVIWQSPLWNNHTTVMATAPDGSFGGVIREGNSTYRVQFYDGNGTPT
                     SLTPALALPIQWIHSLDFYEDGSFLISGTTLTQRGFMARIQRDGQVLWSAESPEDGQE
                     HIQISSGIATPDGWALGTGYSASPRFAALRVSENSGIVVNTLSGRVGHDQDEDCTLQS
                     DEPSMFYQRVEARQGNSVFYGFTTSTGEYEMLLPAGHFTMSVSSNEPFFELCPDATWE
                     VNFPAGQNNELTLDLPMQSADLVHHLSGYVKWDQNDNCTSETSDTPLENWWVRVNFGG
                     HSVSKRTNAQGYYQLFLPSGEYQVSVFPFNHLFETCAPEVQNVVFSGNDPQSATADFT
                     ATSDNDCAYMHVRIGAGSVRPCVNSTFPVYYKNEGVRSAENVSVEVTLPPSLEFVSAE
                     PAPTSVNGNVLRFEFDEVPASPGGYSSMILITVHPDCNLQIGDQVCLNAQITPAEMCG
                     DAPGWNGAVTSLSAECVGDSAVFKIKNIGTAPHSGQRNFRIVEDQIVLLQGTFQLDPL
                     AEDRYAIFIENDSIASFTMIADQEPGFPGDPEVVLTLGNCTTSGGNPSGFGGNPGPFS
                     ASGCLSVRNSYDPNDKQAFPLGFGEDKAIRPGTPIDYTIRFQNTGNDTAYVVVLRDTL
                     SEHLDYTKVELAGASHRFEFAQLGEGNVLHFRFPGINLPDSTTNPAGSQGFVQFRVYP
                     KADLPSGTVVDNSAAIYFDFNPPIITNTVRRKYQEFTLVQTLEPKQPGFLNVRVFPNP
                     FMERAVLSLPDEAPDGNYRLELFDAAGRLLQRKDFRERQVEINGEALPSGAMFWTVSS
                     EGRVVATGKVLR"
     gene            6382..7803
                     /locus_tag="PK198_13195"
     CDS             6382..7803
                     /locus_tag="PK198_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39739.1"
                     /translation="MQDSALFEAIQSLDAWEARRFRLRLQSPFFNHKPTLLQLFEYLW
                     QCRSSDSTPSQTGAMATVGADEVSSPARLRHEMSELLALLREYLTDVELEKTPWQRHL
                     AQVRALRKRGLEKNFRHAVREAQKTSPALETAAAERSLLDYYLQTELYEWEIRSKRYL
                     DFSFHEQAGHLNIWYAGQLLQMACSEMAQAALQHNSTAPHRLADWVEGVLAALPDAPQ
                     LRDPATALYYGGLQMLKAPENSALAEHFRDLLEQHIDRLSHSEARELLLLAINHGIRR
                     INAGEREALRRTLDFYLLGLRQKLLLDENGLLSKFTYNNVLMTFLALEAWEQALEFLE
                     QYRPQLPPKEQDNLFRYNMAVYRFRKGEFDQALELLRNVNFPDPMYNLESRKMLLKIY
                     YEQQAFDALESLLENLLTWLRRHSEIGYQREMYRNLARFTGQLLRLPPHDREAALRLA
                     QKIRDTPMLAERNWLLEKLENNR"
     gene            complement(7943..8473)
                     /locus_tag="PK198_13200"
     CDS             complement(7943..8473)
                     /locus_tag="PK198_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39740.1"
                     /translation="MSKKKFTDGWEGLFESASDHASGQTLLLFDLPESETAKPQRGAK
                     SPVAEAAPELPKHSGKSFASDLESFLQEAFEDSFAEQTRQRTDAEPEPNAKTRRRPAP
                     AARGLDSLIRNTMEETQAGTDHSTAGVRRLVLFLDEQKIARLKSIARTEKTVLRSIVD
                     QIVAEYIADYERRQGE"
     gene            complement(8470..>9185)
                     /locus_tag="PK198_13205"
     CDS             complement(8470..>9185)
                     /locus_tag="PK198_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRF39741.1"
                     /translation="GLAQLGKRTLLIDLDPQASLTISLGIPRQPVTIYEAFRGEAEPM
                     PYAAKTNLDVITSRLELSGAETELLNEAGREYILAELLEPLRDDYEFIVIDCPPSLGL
                     LTLNALTASDYVYIPMQTEFLALQGLAKIKQVIDKVRFRLNKTLEIGGVIATMYDSRK
                     ILNRDVLDMIKKYFGEKVFNTMIRDNVALAEAPSTRQDIFSYSPSSPGAEDYLNLCRE
                     MLERFEQPSAREISREQRV"
BASE COUNT         2075 a         2375 c         2446 g         1989 t
ORIGIN      
        1 cacgcggagg gtcagttcga tgaaatcctg tacggagagt tgctccggcc tttttagaaa
       61 aaaatcatct tgcagcgctt cgggctgctg tgaaaaaaac ggacgcagcg tattgcgcaa
      121 cattttgcgc cgttgcccga aggttatttt tacgaccgaa cgaaacaggg cctcatcgca
      181 aggcagagag ggctgcggtt ttcgcaacaa gcggatgacg cccgactgca cttgcggcgg
      241 cggctgaaac gcgctcttgt ccacactgaa caggtatttc ccttcgtaaa atgcctgaag
      301 caatacgctg atgacgccat agtccttgtt gcccggcccc gataccaccc gcacnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn agacaagtcc acctgcagaa aatcggctga aataacccgc
      541 aagccttcca atggcggacg cgcatcaagg tactccacca tgtcccggtc ggcttctacg
      601 gccaccactg ctctgccggg cgcgcccaaa tgtttggtga gcatgccttt gcccggccct
      661 acctccagta cccgttcgta aggctgcgtg agggaggcgg caatgcgggc ggcaatgtcg
      721 tcgcggtgca ggaaatgctg tccgtaagat tttttcgctt tcaaacgcaa tcgggttgaa
      781 tgttctatac tcgatgaaaa atggtttgca aaacaatata aaccattttt catcgggata
      841 actgatagcc agtggtttgc aaaatctaaa ttcgcaaacc actggccatc gagtataaat
      901 gtcgcgcaaa ataactacct ttgcccatct tgtgaaaaca aataacatgg aattacccaa
      961 aataggcatc accgtaggcg atatcaacgg catcgggctg gaagtcatca ttaagacctt
     1021 gagcgaaaag aagattttag actactgcgt gccggtcata tatggctcct cgaaggtggt
     1081 atcgtatcac aaaaacatcg taggcgacga ctttcagttt gtgcccatcc gcagcgccga
     1141 gcgcatcacg ccggagcggg tcaatgtagt caactgctgg aatgaagtgg tcaatatctc
     1201 tctgggcaaa gtagtgctcg aaggcggtca atacgctttc aaagcattgg aaatggcggt
     1261 gggcgaactc aaagcaggat tcattgacgc tttggtcacg gctcccatca gcaaagaagc
     1321 catgcaaatg gccggtttcc cgtttccggg ccataccgaa tacattgcca aagagctgag
     1381 cgctcccgaa agccttatgc tgatggtgag cgatgcgctg cgcgtgggtt tggccaccaa
     1441 tcacctgcct atcagagaag tagcgggcgc cattaccaaa gaagtattgc tgaaaaaaat
     1501 tcagatattt catgaaaccc tccagattga cttcgacaaa gaccgcccca ccattgctgt
     1561 gatgggtctc aatccgcacg ctggcgaaaa cggcatgttg ggcgaagagg aggaaaaaat
     1621 cattcgcccg gccattgtgg agtgcaaaaa gaaaggcatc gtagtgaacg gccccttctc
     1681 cgctgacggg tttttcggct ccggccaata ccacaaatac gacgggattc tggccatgta
     1741 ccacgatcag ggcctgattc ctttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     1921 gaaatcgccg caactacttc gaaatgcgcg ccaacccctt gcgcaaacgc gaaaaacccg
     1981 ccgaagtgga agaatctcag atcgtgcaag agtaaagcct gaatgcagca cagcatggaa
     2041 gacaaaagga aattgagatt actggctgcg gtcgtgatga ccgttttgtt agtggaccaa
     2101 agcgtcaagt tttggattaa aaccaacatg agcattggcg aagaaatgta tcccctgggc
     2161 gtgaaatggg ccgttattca cttcgttgaa aacccgggca tggcattcgg gcttaccttc
     2221 ggcggcgagt acggcaagct ggcgctcagt ttgtttcgca tcgtagcggt cgtttggttt
     2281 ttatttgcgt tggctgtatc aagagcgcgt ggcgggcata atgctggcgg gctttggcct
     2341 cattctggcg ggcgcgctgg gcaatatcat agacagcgct ttttacgacc tgattttttc
     2401 ggaatccacc tatcatagcg atccggccat cctgttcccg gatgaaggcg gctatgccgg
     2461 atttttgcat ggcaaggtag tggatatgct ctatttccct attgccgagg gctattatcc
     2521 cgactggttt ccgttctgga gcggcgagcc gtatcagttt ttccgcccgg tgttcaatgt
     2581 ggcagatatg gccatctcct gcggtgtaac attagtgctc attctgcatg ttttcttttc
     2641 caaaatgatt catcccatgc ctgctccggc tcttgttacg catgcaacaa atgcggaaac
     2701 cgctgtaatt gaagacactt cgacggaaga aaacgatccg gccgactcct ccgatcccga
     2761 tgaagcctcg caggaccccg tgattcctgc ctgataaagc cccccaaaca aagagcgtcg
     2821 cccggagata cctccgaacg acgctttgct gaattttcac aggccgttag tagtggggca
     2881 aaaagaacca ctttattttt ttatcgcccc ttacctcagc acttttcctg tggccacaac
     2941 ccttccttcg gaagaaaccg tccagaacat agccccggaa ggcagcgcct cgccgttgat
     3001 ttctacctgg cgctcgcgga agtctttccg ctgcaagagt cggcccgccg cgtcgaacag
     3061 ttccaatcgg tagttcccat caggggcctc atcgggcagg ctcaaaacgg cgcgttccat
     3121 gaagggattg ggaaatacgc gcacgttgag gaagcccggc tgtttcggtt cgagggtttg
     3181 taccaaagta aattcctgat atttacgacg gactgtattg gtgatgatcg gcggattgaa
     3241 atcgaaataa atggccgccg aattgtccac caccgtacct gaaggcaggt cggctttggg
     3301 gtaaaccctg aactgtacga agccctgcga tcctgcggga ttggtagtgc tgtcgggcaa
     3361 gttaatgccg gggaagcgga agtgcagtac attgccctcg cccaattgag caaactcgaa
     3421 gcgatggctg gcgccggcaa gttccacttt cgtgtaatcc aaatgctcag agagcgtatc
     3481 gcgcagcact accacatagg ccgtgtcgtt gcccgtattc tggaaacgga tggtgtaatc
     3541 tatgggcgtg ccgggacgaa tggccttgtc ttctccgaag ccgaggggga atgcctgctt
     3601 gtcgttgggg tcgtaagaat tgcgcaccga gaggcatcca cttgcagaga acggtccggg
     3661 attgccgcca aaaccggagg ggttgccgcc cgacgtcgtg caattgccga gcgtgagtac
     3721 cacttccggg tcgccgggga agccaggctc ctgatcggcg atcatggtga aggaggcgat
     3781 ggaatcattt tcgataaaaa tagcataccg gtcttctgcc agaggatcaa gttggaacgt
     3841 accctgtaag agcacaatct ggtcttccac gatgcggaag ttgcgctgtc cggagtgcgg
     3901 cgccgtaccg atgtttttga ttttaaacac ggcgctgtca cccacacatt cggcgctcag
     3961 gcttgtcacc gcgccgttcc agccaggtgc gtcgccacac atttcggcag gcgttatctg
     4021 ggcgttcagg catacctgat cgccgatctg aaggttgcaa tccgggtgta cggtgatgag
     4081 aatcatgctg gaatacccgc cgggagaggc cggcacctca tcgaattcaa agcgcagcac
     4141 attgccgttg actgaagtcg gggcaggctc ggcactcaca aattccagac tgggcggcag
     4201 tgttacttct acgcttacat tttcggcact gcggacgcct tcatttttgt aataaaccgg
     4261 aaacgtagag tttacgcaag gccgaacgct tcccgcaccg attcgcacat gcatgtacgc
     4321 acagtcgttg tcggaggtgg cggtgaaatc agcagtggca gattgtgggt cgttgccgct
     4381 gaaaacgacg ttttgtactt ccggcgcaca agtctcaaac aagtgattga aaggaaatac
     4441 gctcacctga tactcgcccg aaggcagaaa aagctgatag tacccctgcg cgttggtgcg
     4501 cttggaaaca gaatgcccgc caaaatttac ccgcacccac cagttttcaa ggggcgtatc
     4561 ggaggtctcg ctggtgcagt tgtcgttttg gtcccacttc acatagccgc tgaggtggtg
     4621 taccagatcg gcgctctgca tgggcaggtc gagcgtgagt tcgttgttct ggccggcggg
     4681 aaagttgacc tcccaggtcg catcggggca aagttcgaag aaaggctcat tggaacttac
     4741 gctcatcgtg aaatggccgg ccggcaacaa catttcatat tcgccggtgc tggtggtgaa
     4801 accataaaaa accgagttgc cctggcgggc ttccacgcgc tggtaaaaca tgctcggctc
     4861 gtcgctctgc aaggtgcaat cttcatcctg atcgtgcccg acgcgaccgc tgagggtatt
     4921 gaccacaatg cctgaatttt cggaaaccct cagcgcggca aacctcggcg aagcgctgta
     4981 ccccgtgccg agtgcccagc cgtcgggagt ggctatgccg gaactgattt gtatgtgttc
     5041 ctgcccgtct tccggtgatt ctgccgacca aagcacctgc ccgtcgcgct gaatgcgggc
     5101 cataaagccc cgttgtgtga gcgttgtgcc gctgatgaga aaagagccgt cttcataaaa
     5161 atccaaagaa tgtatccatt ggatgggcag cgccagggcc ggtgtcagtg aagtaggcgt
     5221 gccgttgccg tcataaaatt gtacgcggta tgtgctgttg ccctctctga tgaccccgcc
     5281 gaagctgccg tccggcgcgg tggccattac cgtggtatgg ttgttccaca aagggctttg
     5341 ccagatcacc tgattgttct gagcaattcg tgaaaatacg gccttgcctt gcgaaaaatg
     5401 caatgtgagc gcatcaccgt tgggaaacac atctgaatgt gcgagattgg gcagcgaaat
     5461 gatgggctgg tattgcactt catggccgtc cagatcaatt tcgagggtgc cgcaggagtt
     5521 gccgggaaac tggaaccggc agtgtgtgac aaaaatatgg ccattgggca acacccccag
     5581 cgggtaggtg gcagaggaaa agccgggcag atcaatgccc cacaaaaact cgccggcgcc
     5641 ggaaatgcgc accagatcga aatcagaggt agaatcctga acggcaaaca caaagcttcc
     5701 gtcgggcaat tccaaggggg cgctgctcgc ttctgcaatg atataaggct gaggagcagg
     5761 caaataagtc tcatcccaga ctacctgcaa cccggcatcg aggcgaatgg cgcgaggcaa
     5821 ttgaacgtaa gagttgctgg aagaggctgt ggccgacccc aacaggatgt agcctccgtc
     5881 gttggtggcc cgcacaaatt gacccgaaga ctggctgaag cccgccggat gtggaaaaag
     5941 tattttggtg ttttgctgct gtgcctgcaa aacgcccgca ctcaacaggc aaatccatgc
     6001 ccagaaatgg aaggcaaaaa gtcgaaattt cttattcatg gcgcgtattg gttttgatac
     6061 ggtacaaaag tgcgggcaaa acaggagccg ggcaaaatca ttttcgggaa tttgtcggta
     6121 agctttttta aaagatgcgg ataaaatctc tgtatttaag gcaatccatc acatagctat
     6181 caggaatttc caacaaaagt cagataattt ttcagaccgg gtttatcttt gatttttgaa
     6241 ttttcgcaat cctttgtaca catgattgcc accatcacct ccctgcatgg cctcaagcgg
     6301 cttctgttcg gtaaacaaaa ccgatggctt ctggcgtccg gcggcgtatc tgctggtaga
     6361 tcatgactaa aacagcgagc aatgcaggat tccgcacttt ttgaagccat acaatcgctg
     6421 gatgcctggg aagcgcggcg gttccgcttg cgcctgcaat ctccgttttt caatcacaaa
     6481 ccaacactgt tgcagttgtt cgagtatttg tggcaatgcc gctcatccga ctccacgccc
     6541 agtcagacag gcgcgatggc tactgtcggc gccgatgaag tcagctcgcc ggcccggctg
     6601 cgacacgaaa tgagcgagtt gctggccctg ttgcgcgaat acctcacgga tgttgaatta
     6661 gaaaaaacac cctggcagcg acatctcgcg caggtgcggg cgctgcgcaa acgcggcttg
     6721 gaaaaaaact tccgccatgc cgttcgggag gcgcaaaaga caagcccggc cctcgaaacc
     6781 gccgccgccg agcgcagcct gctggattat tacctgcaaa cggagttgta cgagtgggaa
     6841 atccgctcca agcgatattt agatttttcc ttccatgagc aagccgggca tctcaacatc
     6901 tggtacgccg ggcaattgct ccaaatggcc tgcagcgaga tggcacaggc tgccctgcaa
     6961 cacaacagca cagctccgca ccggctcgcc gattgggtag aaggcgtgct ggcggctttg
     7021 cccgatgccc cgcagctccg cgatccggca acagccttgt attatggcgg tctgcaaatg
     7081 ctgaaagccc ctgaaaacag cgccctggcc gagcatttcc gggatttatt ggagcaacat
     7141 attgaccgct tgtcgcactc cgaagcccgc gaactgctgc tgctggccat caaccacggc
     7201 atccggcgca tcaatgcggg ggaacgcgag gctctgcgac gcacgctgga tttctacctg
     7261 ctgggcctgc gccaaaaact gctgttggac gaaaatggcc tgctgtccaa atttacttac
     7321 aacaatgtgc tgatgacttt cctggccctc gaagcgtggg aacaggcgct ggaatttctg
     7381 gagcaatatc gcccgcagtt gcctcccaaa gaacaggaca acctcttcag gtacaacatg
     7441 gccgtgtatc gctttcgcaa aggcgagttc gatcaggcgc tggaactgct gcgcaatgtc
     7501 aatttccccg accccatgta caacctggaa agccgcaaga tgctgctcaa aatttattac
     7561 gaacaacagg ctttcgacgc gctcgaatcg ttgctcgaaa acctgctcac atggctgcgc
     7621 cgccacagtg aaatcggata ccaaagggaa atgtaccgca atctggcgcg cttcaccggc
     7681 caactgctgc gcctgcctcc gcacgaccgc gaagccgccc tgcgattggc tcaaaaaata
     7741 cgcgacacgc ccatgctggc tgaacgcaac tggcttctgg aaaaattaga aaacaataga
     7801 taaagggctt gtcttcggac tcgcattgtg aaatttcccc aagcggaaaa tctccatcct
     7861 gaacaaaaaa acagactcgt agctcatagc tcgcggctcg tagctttttt ggggatgcta
     7921 aatagttctg aggaccaacg acctactccc cctgccgccg ctcataatcg gcaatgtatt
     7981 cggccactat ttgatctacg atgctgcgga gtacggtttt ttcagtgcgg gcaatggatt
     8041 tgagtcgcgc gattttctgc tcgtccaaaa acagcaccaa gcgacgaacc cctgcggtgg
     8101 aatgatccgt gccggcctgc gtttcctcca tcgtattgcg gatgagcgaa tccaaaccac
     8161 gggcagcagg cgcggggcga cggcgggttt tggcattggg ttccggctcg gcgtcggtgc
     8221 gctgccgggt ttgctcggcg aaggagtctt caaacgcctc ttgcaggaag gattccagat
     8281 cggaagcgaa actcttgcca ctgtgtttgg gcagttcggg agcagcctcg gccactggag
     8341 atttggcgcc gcgttggggt ttggccgttt ccgactccgg gagatcaaac aaaagcagcg
     8401 tctggcccga agcatgatcc gaagcagact caaaaaggcc ctcccagccg tcggtgaatt
     8461 tttttttgct catacgcgct gttctctgct gatttctctg gcggaaggct gctcgaaacg
     8521 ctccagcatt tcccggcaca ggttgagata atcttccgcc cccggactgg acgggctgta
     8581 gctgaaaata tcctgtcggg tggagggcgc ttctgccaat gccacgttgt cgcgaatcat
     8641 ggtattgaag accttctcgc cgaaatattt tttgatcatg tccaacacat ctcggttgag
     8701 gattttgcga ctgtcgtaca tggtggcaat cacgccgccg atttccagcg ttttgttgag
     8761 ccggaagcgc accttgtcaa tcacctgttt gattttggcc aatccctgca aagccagaaa
     8821 ctcagtttgc atgggaatgt aaacgtaatc gctggcggtg agggcgttga gcgtcagcag
     8881 tcccagcgaa ggcgggcagt caataacgat gaactcgtag tcgtcgcgca gcggctctaa
     8941 tagctcggcc aggatgtact cgcggcccgc ttcgttgagc agttccgttt ctgcgcccga
     9001 caattcgagg cgggaggtga tgacgtccaa gttggttttg gccgcataag gcatcggttc
     9061 ggcctcgccg cggaatgcct cgtaaatggt gaccggttgc cgcgggatgc ccagcgaaat
     9121 ggtgaggctg gcctgcgggt cgaggtcaat gaggagcgtg cgtttgccca attgcgccaa
     9181 gcccg
//
LOCUS       DAORLF010000158         9159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4082_length_9159_cov_2.392465, whole genome shotgun sequence.
ACCESSION   DAORLF010000158 DAORLF010000000
VERSION     DAORLF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4082_length_9159_cov_2.392465"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>411)
                     /locus_tag="PK198_13210"
     CDS             complement(<1..>411)
                     /locus_tag="PK198_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39742.1"
                     /translation="GDITPITSQNVSICAGTSLQVGNSIYTQSGLYADTIQVFAGCDS
                     IVNTSLTVLPPITLLLSQNLPAFGAGSNNGVATASASGGTGGFTYQWCNGETGPQAVA
                     LPGNQTCSVTVTDGFGCSQVEDIFIDEILPLQPTA"
     assembly_gap    412..423
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<424..>1531)
                     /locus_tag="PK198_13215"
     CDS             complement(<424..>1531)
                     /locus_tag="PK198_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39743.1"
                     /translation="PADVPAQLNLCFRAFENDGLPFTCLITEECAETICQDFAIPASG
                     SANYTLALPAGLSSGGSVSFTITTTPSPLVAYDNICTPIHLGTLNFGQQLGDASLGGY
                     HNRCATNANEPNPLLFVPGWNNEYGVWFTFTTGPDPGSLIILEARDDPQNTGDNMDTQ
                     IAVYRSTNNTCNGAMLFTRAVADWNNPDSRLDLWCPLPNTTYFVLIDGDFIVPGGFQG
                     DFGFQVRSVAVPEAADAPCGAENLGAVPPGGSVETPGQRASFCATSVNDPFVPAFSVQ
                     ASVWFQFTAPPSGHVLIEGISDVTIAPWTIQLAVFASSNNTCSGQLLHVASRFDGSTN
                     DESIQVSCLYPGQPYWVMFDGFGAGGRGIFSLRVS"
     assembly_gap    1532..1545
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1546..1803)
                     /locus_tag="PK198_13220"
     CDS             complement(<1546..1803)
                     /locus_tag="PK198_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39744.1"
                     /translation="MRPFFTQTSSVFLTFLFLFAGISAARAQVTVSVVINSGNATTTC
                     TDLLSPPDPMWRVNIENQGWSTYPRIGNCWTALPNTQYTATY"
     assembly_gap    1872..1917
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1918..2182
                     /locus_tag="PK198_13225"
     CDS             <1918..2182
                     /locus_tag="PK198_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39745.1"
                     /translation="KRIPKQLPKHGDEKMRLLYPAGITMRAGGLVFISPISLPVRPIS
                     AGQGITKLEQKRLCRRHFSNNDRFRYYFSYPVNYIFPNLRLSG"
     assembly_gap    2319..2355
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2356..2627
                     /locus_tag="PK198_13230"
     CDS             <2356..2627
                     /locus_tag="PK198_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF39746.1"
                     /translation="ITFLSVGGLMILSGYRAKLGAFLLLLYWLPVTVIVHAFWKHPPG
                     DTYRWEAMKFMQNIAIAGGLLMVMANGTGAWAIKRIFATARVRGV"
     gene            2670..>2899
                     /locus_tag="PK198_13235"
     CDS             2670..>2899
                     /locus_tag="PK198_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HRF39747.1"
                     /translation="MIKAVIVEDEEKSLINLRNMLAKECPQVEIIGEARKVEEAVDLL
                     SDPAIKPDVAFLDIRLPDGLVFQLLNQVKPVD"
     assembly_gap    2900..2936
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2937..3437
                     /locus_tag="PK198_13240"
     CDS             <2937..3437
                     /locus_tag="PK198_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF39748.1"
                     /translation="QYAIRACEYSSIGYILKPIDPDRLKDAVARIRPRQNNQIESRLD
                     VFSHAYNNPNAFTKLSVFGLDGIYFVNIKDVIRLEAEDNYTHIFMHTGERITASKTIK
                     SYEDLLTPMNFFRVHKRHIINLNFMRKFVRTDGYIVMEDTAKIEVSRRRRPAFVEQMR
                     KLQQVY"
     gene            3568..3726
                     /locus_tag="PK198_13245"
     CDS             3568..3726
                     /locus_tag="PK198_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39749.1"
                     /translation="MGKDKKTLDELKDDLKTINKRDQEKVRGGSSDKEKDKDKRKWDS
                     GLGGIVPQ"
     gene            3829..5757
                     /locus_tag="PK198_13250"
     CDS             3829..5757
                     /locus_tag="PK198_13250"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF39750.1"
                     /translation="MTTATAKPIPAKQLEKKLAKEPHPLRRMELLDKITAHYIYTYTE
                     GALEYLAELEQILQEWPQLDLQLNYHYHRAVVENQLYHFQEAAQHCLDALILAEEQGD
                     TYRRVELYIDFAGIQMNLNRFADAADWLDKAQKMLRQWPDERLSSRYKCREGFLQLHY
                     GDLSAATELLLDADKSILRLSPLQLKDHFFLSLIYSGLGTVYERSGDMERSVEAYRKV
                     VKTCETTGIQARMDWHYLKLGAGLMALSEYEEAESFFEKVLHLGSQAGASARAGALGN
                     LGYCRFQRKRFDEALDLFQQAEDLFKTKPADAAAYNFSIIEGWRGRLYAETGQTRKAK
                     EHFAKAVEFARQNEDYKQISSICRDVAAFYAARRDYKNAYEFQLIYDQMAERYREQIN
                     QQRLMELEVRYEAEKKKQEAEMLKLQASKLQLKALRAQMNPHFMYNALNAIQHFITSN
                     ETAAASKYLARFAQLMRRSLEYSDLEFIALEKEIEFLEDYLFLNEKLRFEGRLQYRIS
                     VDDDIEEDISGVPAMIVQPYVENALEHGLRIKEHGTVEVRFSLFDEDTILCTVEDNGI
                     GREKARQMQKASGYFNDHRSRGTSITRKRLQLLHQSKQNKGIFVRIIDLRDESTGEAA
                     GTRVEIKIPIREVSGRGE"
     gene            complement(5822..6817)
                     /gene="trpD"
                     /locus_tag="PK198_13255"
     CDS             complement(5822..6817)
                     /gene="trpD"
                     /locus_tag="PK198_13255"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRF39751.1"
                     /translation="MKTTLARLFDHEYLSRDEAAAVLTEIAAGAHNEVQVAAFITVYQ
                     MRPPSVHELLGFRDALLHLAVPFHLDGLQSIDIVGTGGDHKNTFNISTLSAVVVAGAG
                     YKVTKHGSYGVSSAVGSSNVLSALGYQFTNDTNVLRRQLDEAGLCFLHAPLFHPALKE
                     VAPVRRQMGMKTIFNLLGPLVNPVQPTHNLFGTSGLEIARLYQYVMQETGRRFIIIYA
                     LDGYDEISLTGPFKVYRNTGEQTLAPEDIGAAGVAPEDLFGGDTEAEAAEIFLAVLDN
                     RATPAQTEVVCANAGMAIHCFHPERSVEDCMAEARESIVSGQAKKTLERLIVSGL"
     gene            6984..8426
                     /gene="gatB"
                     /locus_tag="PK198_13260"
     CDS             6984..8426
                     /gene="gatB"
                     /locus_tag="PK198_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRF39752.1"
                     /translation="MTAATASYETVIGLEVHVQLATHSKAFCGDDASFGGGPNTHVGA
                     ISLGHPGTLPMLNRRQVEFAVALGSRINPRNAFDRKNYFYADLPKGYQITQDRQPVCI
                     GGALEIRVGHQSKSVRIHHIHIEEDAGKSMHDQDAHNSLIDLNRAGVPLLEIVTEPDL
                     RSGEEVDAFMTAMRRLVRYLEISDGNMEQGSMRCDVNVSVRPQGETRFGTRCEVKNLN
                     SMKFARQAIDYETQRQVNLIESGGQVEQNTLHFDPATGITSPLRSKEDAHDYRYFPDP
                     DLPPVVLDEAYIEGVRAQMPPLPQQLFEQFTQQYNLSEYDAALLTEEKPVALYFIELC
                     RHTPHVKAAANLIINKIMPWRDEHKAGIEAYPVSQERLAGMLELVAGGQVSNTAAWQN
                     LLPAMMEKPESGAPELAQMLGLLQTNDESFLSEIIDQALAANPNKVAEYRKGKKGLQG
                     FFMGEIMRLSKGKADPKAATPLLLQKLDAS"
     gene            8423..8755
                     /locus_tag="PK198_13265"
     CDS             8423..8755
                     /locus_tag="PK198_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF39753.1"
                     /translation="MMFENQDDLKRYYSIGEVAELFDVSRSLIRYWEAEFDNIRPQKN
                     ARGERRYTKANIEQIRLVYELVKERGFTIAGARQELKERREYRRERDAVVGRLKELRG
                     FLEKLARQ"
     gene            8911..>9159
                     /locus_tag="PK198_13270"
     CDS             8911..>9159
                     /locus_tag="PK198_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF39754.1"
                     /translation="MKRPDFSQINCRRPESLQQPSASQEWPTAEHIPVKSAYTPEDRQ
                     GLSHLHFAAGLPPYLRGPYASMYTVRPWTVRQYAGFSTA"
BASE COUNT         2203 a         2385 c         2495 g         1930 t
ORIGIN      
        1 gcggtgggtt gcagcggcag tatttcatct ataaaaatgt cttccacctg cgagcagccg
       61 aagccgtcgg tcaccgttac cgagcaggtc tgattgccgg gtagagcaac tgcctgaggg
      121 ccggtttcgc cgttgcacca ttggtaggta aaaccgcccg ttccgcccga tgcgcttgcc
      181 gtagccacgc cgttgttgga acccgcgccg aaagcaggca ggttttggct cagtagcaaa
      241 gtgatgggcg gcaacacggt gagcgaggtg ttgacgatgc tgtcgcaacc ggcaaaaact
      301 tgtatggtat cggcatacag gcccgattgg gtataaatgg aattgcccac ctgaaggctg
      361 gtacccgcgc aaatactcac attttggctg gtgataggcg tgatgtcgcc tnnnnnnnnn
      421 nnngctgacg cgcaaggaaa aaatgccgcg cccgcctgct ccgaacccgt caaacatgac
      481 ccagtagggt tggccgggat agagacaact gacctgaatg ctctcgtcgt tggtgctgcc
      541 gtcaaaccgg ctggcaacgt gcagtaactg gcccgaacaa gtattattgg acgacgcaaa
      601 taccgccaac tggatggtcc agggtgcaat ggtcacatcg ctgatgcctt cgatgaggac
      661 gtggccggag ggcggcgcgg tgaactgaaa ccacacggag gcctgcaccg aaaatgccgg
      721 cacgaaagga tcattgacac tggtggcgca aaaactggcc cgctggccgg gcgtttccac
      781 cgaacctccg ggcggcaccg cgccgaggtt ttcagcgcca cagggagcgt cggcagcttc
      841 gggaacggct acgctgcgca cctgaaaccc gaaatcgccc tggaacccgc ccggcacgat
      901 gaagtcgccg tcaatgagta caaagtaggt ggtattgggc agtgggcacc agaggtccaa
      961 gcggctgtcg ggattgttcc agtcggctac ggcgcgggta aagagcatgg cgccgttgca
     1021 ggtattgttg gtggagcgat acacggcgat ctgggtgtcc atattgtcgc cggtattttg
     1081 ggggtcgtcg cgggcttcga ggatgatgag cgaaccgggg tctgggccgg tggtgaaggt
     1141 aaaccagacg ccgtactcgt tgttccagcc cggcacgaag agcagcggat tgggttcgtt
     1201 ggcattggta gcacagcggt tgtggtagcc gccgagggaa gcgtcgccca attgctgccc
     1261 gaaattgagc gtgccgagat ggatgggggt gcagatattg tcgtaagcca ccaaaggcga
     1321 gggcgtggtg gtgatggtaa aactgacgct gccgccggaa ctgaggcctg cgggcagggc
     1381 gagggtataa ttggcgctgc cggatgcggg tatggcgaag tcttggcaga tggtttcggc
     1441 acattcctcg gtgatgaggc aggtgaaagg caagccgtcg ttttcaaaag cgcggaaaca
     1501 gaggttcaac tgggccggca catcagcggg gnnnnnnnnn nnnnngtaag tggcggtata
     1561 ttgggtattg ggcagggccg tccagcagtt gccgatgcgc gggtaggtac tccagccctg
     1621 gttttcgatg ttgacgcgcc acatagggtc gggcggggag aggaggtcgg tgcaggtggt
     1681 agtagcgttt ccgctgttga tgaccacgct gacggtgact tgggcacggg ccgccgaaat
     1741 accggcaaac aggaacaaaa aagtaagaaa aactgagctt gtttgggtaa aaaatggacg
     1801 catgagcaag gatggttttt gtcaaaaata cttattggaa cgactgttgc ctgttggaat
     1861 ggctgctggc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     1921 aagaattcca aagcagttgc cgaaacatgg agatgagaag atgcggttgc tatacccggc
     1981 aggcataacg atgcgagcgg gcggtttagt ttttatctcc ccaatttccc tgcccgtacg
     2041 ccccatatcg gccgggcaag gcataaccaa gcttgaacaa aagaggcttt gccgacgcca
     2101 tttttcaaat aatgaccgtt ttagatacta ctttagctac cccgtcaact acatttttcc
     2161 taacttgcgc ctttcgggat agaaaccgtg tcggaatgaa aaaccttaca gacttattag
     2221 gcagaatgct gctttcgttc atattcctgt atgaagccta cgatatagcg agttatgtgc
     2281 atcataccaa aggcgtgatg atgcaatacg gcattgtgnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnccatc acttttttgt ctgtcggcgg cctgatgatc ctgagcggct
     2401 atcgggccaa gctgggcgct tttttgctgt tgctctattg gttgcccgtg acggtcatcg
     2461 ttcatgcctt ctggaaacac ccgcccggcg atacataccg ctgggaggcc atgaaattca
     2521 tgcaaaacat agccattgcc ggcggcctgc tcatggtgat ggccaatgga accggcgcct
     2581 gggccattaa aagaattttt gccactgcga gggtcagggg agtgtaagta gttatatatt
     2641 tgcatttttt taatcaatcc aatttcgtcg tgatcaaagc tgtcatcgta gaagatgagg
     2701 agaaaagcct catcaacctc cgcaacatgc tggccaagga gtgcccgcag gtagaaatca
     2761 tcggagaagc ccgcaaggta gaagaagctg tggatttgtt gagcgatccg gccatcaaac
     2821 ccgatgtggc gtttttagac atccgcctgc cggacggctt ggtttttcaa ctgctaaacc
     2881 aggtaaaacc cgttgatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaat
     2941 acgccattcg ggcctgcgag tacagctcca tcggctacat cctcaaaccg attgaccccg
     3001 accggctcaa ggatgccgta gcgcgcattc gcccgcgtca gaacaaccag atcgaatcgc
     3061 gtttggacgt tttttctcat gcctacaaca accccaatgc cttcaccaaa ctcagcgttt
     3121 tcggattgga cggcatctac ttcgtcaaca tcaaagatgt cattcgctta gaagcggaag
     3181 acaactacac gcatattttt atgcacacag gcgagcgcat cacggcctcc aaaaccatca
     3241 aatcttacga agacctgctc acgcctatga actttttcag ggtgcataaa cgccacatca
     3301 tcaacctcaa ctttatgcga aaatttgtgc gcaccgacgg ctatatcgtc atggaagata
     3361 ctgcaaaaat tgaagtatcg aggcgcagaa ggcctgcttt tgttgagcaa atgcgcaagc
     3421 tccagcaggt ctattgagct attatgaata aaaaaatgta ttgtactcag acctgcacgc
     3481 ttgcttgaac gcatctttgt gcgtaccttt ggcaacgaaa atttgaggag caggtaatca
     3541 tttacacaca agactcaatc ttttactatg ggaaaggata aaaaaacctt ggacgaactc
     3601 aaggacgacc tgaaaacgat caacaaacgc gatcaggaaa aggtacgagg cggcagttcc
     3661 gacaaggaaa aagataaaga taaaagaaaa tgggacagcg gtctgggagg tattgtgcct
     3721 cagtaaagac ccgctttttt gtccggccat ccaaagggac tgctccaaac acgggcagtc
     3781 cctttgttgt ttcatgtacc gagttcacac agcatcacac cgaaaacaat gacgacagca
     3841 acagccaaac cgatacctgc caagcagttg gaaaaaaaat tggccaagga accccatccc
     3901 ttgcgcagaa tggagttgtt ggataaaatc accgcacatt acatttatac ttatacagaa
     3961 ggagcattgg agtatcttgc cgagttggag cagattttgc aggaatggcc tcaactcgat
     4021 ttacaattga actaccacta tcatcgggcg gtggtggaaa accagttgta ccattttcag
     4081 gaggccgccc agcattgcct cgatgccttg attctggccg aagagcaggg cgacacttat
     4141 cgcagggtag agctgtacat tgattttgca ggcatacaaa tgaacctcaa tcgctttgca
     4201 gacgctgccg actggttgga caaagcgcaa aaaatgctgc gacagtggcc cgacgagcgc
     4261 ctctcctcgc gctacaaatg ccgggagggc ttcctgcaat tgcattacgg cgacctgagc
     4321 gcagctaccg aactgctctt ggatgccgac aaatctatcc tcagactttc gccgcttcaa
     4381 ctcaaggatc atttttttct ctccttgatt tacagtggat taggtaccgt gtatgagcgc
     4441 agcggcgaca tggagcgcag tgtggaggct taccggaagg tggtgaaaac ctgcgagact
     4501 accggcatcc aggcgcggat ggactggcac tacctcaagc tcggcgccgg gctgatggcc
     4561 ctgagcgagt acgaagaggc ggagtcattt tttgaaaaag tgctgcactt gggcagtcag
     4621 gccggagcat ctgcaagagc cggggcgctg ggcaatctgg ggtattgccg gtttcagcgc
     4681 aagcgttttg atgaagccct cgatctgttt cagcaggccg aagacctttt taaaaccaaa
     4741 cccgccgatg ccgccgcgta caatttttcc atcattgagg gctggcgggg ccgtttgtat
     4801 gccgaaaccg ggcaaacccg caaagccaaa gaacactttg ccaaagcggt cgaatttgcc
     4861 cgtcagaatg aagactacaa acagatttcg agcatctgtc gcgatgtggc cgcgttttat
     4921 gccgcccgcc gggattataa aaatgcctac gaatttcagt tgatttacga tcaaatggcc
     4981 gagcgctacc gggagcagat caaccagcaa cgcctcatgg agcttgaagt gcgctatgag
     5041 gccgaaaaga aaaaacagga ggctgaaatg ctcaaattgc aggcctccaa actacaactc
     5101 aaggccctgc gcgcccagat gaacccccac ttcatgtaca atgcgctcaa tgccattcag
     5161 cattttatca cctccaacga aaccgctgcg gcatccaaat atctggcccg ttttgcccag
     5221 ctcatgcgcc gcagtctgga gtattctgat ctggaattca ttgccttaga aaaagaaatt
     5281 gagtttttgg aagactacct cttcctcaac gaaaaactgc gcttcgaggg gcgtcttcag
     5341 tatcgcatca gtgtggacga cgacatcgaa gaggacatca gcggcgtgcc ggccatgatt
     5401 gtgcagcctt atgtggaaaa cgccctcgaa cacggcttgc gcatcaaaga gcacggcacg
     5461 gtggaggtgc gtttttcatt gtttgacgaa gacaccattc tttgcaccgt agaagacaac
     5521 ggcatcgggc gggaaaaggc ccggcaaatg cagaaggcca gcggctactt caacgatcac
     5581 cgctcgcggg gtaccagcat cactcgcaag aggctgcaac tactgcacca gtcgaagcaa
     5641 aacaagggca tttttgtgcg catcattgac cttcgggacg aaagcaccgg cgaagctgcc
     5701 ggcactcgtg tggagatcaa aatcccgatt cgggaggttt cggggcgggg cgagtgaggt
     5761 tgtggctaca gatgttccga acgctgcgga gctgcgctct tctttttcgc cttgagcctt
     5821 tttacagtcc ggatactata agccgctcta atgtcttttt agcctgcccg ctcacaatac
     5881 tctccctggc ctccgccata caatcctcta cggagcgttc ggggtgaaaa cagtgaatgg
     5941 ccatgccggc gttggcacac accacttctg tctgagccgg cgtggcccgg ttgtccaaca
     6001 ccgccagaaa aatttcagcc gcttccgctt cggtatcccc tccaaacaag tcttcgggcg
     6061 ctacgccggc agcgccgatg tcttcggggg ccaaggtttg ctcgcctgta ttgcgataca
     6121 ctttgaaggg gccggtgagc gagatttcgt cgtagccgtc caatgcgtaa atgatgatga
     6181 agcggcgccc ggtttcctgc atgacgtact gatacaggcg ggcaatttcc agccccgaag
     6241 tgccgaacaa gttgtgcgtg ggctgcaccg gattgaccaa tggccccagc agattgaaga
     6301 tggttttcat acccatttgc ctgcgcacgg gcgccacctc tttgagtgca ggatggaaca
     6361 agggcgcatg taaaaagcaa agccccgcct cgtccaattg ccgcctcagc acattggtgt
     6421 cattggtaaa ttggtagccc agcgcgctga gcacattgga agaacccacc gccgaggata
     6481 cgccgtagct gccgtgtttg gtcactttgt aacccgcgcc ggccactacc accgccgaaa
     6541 gcgtggagat gttgaacgtg tttttatggt cgccgccggt acccacaata tctatacttt
     6601 gcaagccgtc cagatgaaac ggaacggcca aatgcagcag cgcatcccgg aagccgagca
     6661 gttcatgcac tgatgggggg cgcatctgat acacggtgat gaaagcggct acctgcacct
     6721 cattgtgcgc gccggcagcg atttcggtga gtaccgcagc ggcttcatcc cggctcaggt
     6781 attcgtggtc aaacagtctg gcgagtgttg ttttcatata agtttcagag aatgtgtcgg
     6841 gccgcaaagg taagtacaag tttcggtttg cccgcgctgg agccgagtcg tgggatactc
     6901 ttgcgaaaag cgccctctcc attcggcagg tctcagtaat tattccgaca tttgcgggag
     6961 ttcctgattt tgatacacaa cgaatgaccg cagcaacagc atcttacgaa accgtcatcg
     7021 gcttggaggt acacgttcag ctcgccacgc acagcaaagc cttttgtggc gacgacgctt
     7081 cattcggggg aggccccaac acccacgtcg gagccatctc actggggcat ccgggtactt
     7141 tgcccatgct caaccggcgt caggtggaat ttgccgtggc gctgggcagc cgcatcaacc
     7201 cgcgcaatgc cttcgaccgc aaaaactact tctacgccga tttgccgaaa ggctaccaaa
     7261 ttacacaaga ccggcagccc gtttgcatcg gcggggcttt ggaaatacgc gtcggccatc
     7321 agtccaaatc ggtgcgcatc caccatatcc atatcgagga agacgccggg aaaagtatgc
     7381 acgatcagga cgcgcacaac tcgctcatag acctcaaccg ggccggtgtg ccgctgttgg
     7441 aaatcgtgac cgaacccgac ctccgttccg gcgaagaagt ggatgcgttt atgaccgcca
     7501 tgcgccgatt ggtgcggtat ttggaaatca gcgacggcaa tatggagcag ggttccatgc
     7561 gctgcgatgt caacgtttcg gtgcggccac agggcgaaac ccgcttcggc acccgctgcg
     7621 aggtcaagaa tctcaactcg atgaaatttg cccgtcaagc cattgattat gagacacaaa
     7681 ggcaggtaaa cctcatcgaa tcgggcggtc aggtggagca aaacaccctg cattttgacc
     7741 ccgctaccgg catcacttcg cccctgcgta gcaaagaaga cgctcacgat tatcgctatt
     7801 tccccgatcc cgacctgccg ccggtggttt tggatgaggc ttatatcgaa ggggtgcgcg
     7861 cccaaatgcc gcccctgccg cagcaacttt ttgagcaatt tacacagcaa tacaatctga
     7921 gcgagtacga tgccgccctg ctcaccgagg aaaaaccggt agctttgtat tttattgaat
     7981 tgtgtcgcca tacgccgcat gtcaaggccg ccgccaatct cattatcaat aaaataatgc
     8041 cctggcgcga cgaacataaa gcaggcatag aggcgtaccc ggtctcgcag gagcgcctcg
     8101 cagggatgtt ggagttggtg gcaggagggc aggtgagcaa cacggcagcc tggcaaaacc
     8161 tgttgcccgc catgatggaa aaaccggaat cgggcgcgcc ggaactggcg caaatgctgg
     8221 gcttgctgca aacgaacgac gaaagtttcc tttcggaaat catagatcag gcattggccg
     8281 ccaacccgaa caaagtggcc gaataccgca agggcaaaaa aggcttgcag ggatttttta
     8341 tgggcgaaat catgcggctc tcgaaaggca aagccgaccc gaaagccgct actccattgt
     8401 tgttgcaaaa actcgacgcg tcatgatgtt tgaaaaccag gacgatctga agcgctacta
     8461 ctccatcggg gaggtggccg aactgttcga tgtttcgcgc tcgctcatcc gctattggga
     8521 ggctgagttc gacaacatcc gtccgcaaaa aaatgcccgg ggcgaacggc gctacaccaa
     8581 ggccaatatc gagcaaatcc gattagtgta tgaattggtc aaagagcgag gcttcaccat
     8641 tgccggcgcc cggcaggaac tgaaagagcg ccgcgagtac cgccgggagc gcgacgccgt
     8701 tgtcggcagg ctcaaagaac tgcgcggttt tttggaaaaa ttggcgcggc aataaagtta
     8761 aatttctgtg cgtagtgaag aggtgactac gggttgcgac ccaggctgag tcaccccttc
     8821 actaaactac ccaaataaac atatttaatt tacactgcaa taaggccgaa tcgctaactt
     8881 tacgattcca ttttcaaaag caacatctat atgaaacgcc ctgatttcag ccagataaac
     8941 tgccgccggc cggagagcct ccaacaaccg tctgcatcgc aggaatggcc caccgccgag
     9001 cacattcccg tcaaatcggc ctatacgcct gaagaccggc aggggctttc ccatctgcac
     9061 tttgccgccg gactgccgcc ctacctgcgc ggcccgtatg cctccatgta caccgtgcgg
     9121 ccctggacgg tgcgccaata cgcggggttc tctactgcc
//
LOCUS       DAORLF010000159         9158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4083_length_9158_cov_2.269691, whole genome shotgun sequence.
ACCESSION   DAORLF010000159 DAORLF010000000
VERSION     DAORLF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_9158_cov_2.269691"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..718)
                     /locus_tag="PK198_13275"
     CDS             complement(179..718)
                     /locus_tag="PK198_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39755.1"
                     /translation="MSEYKKIYEHLSEQLAQEDIADGYLIPADLTEEERAGAEQEIRE
                     YRLKKLSTRTESERLLSELTRLRLSVHRYLESGVYSSDYSFDEVLKEMIAIMRKTKKE
                     VANDLDVHYTRLSRILNEREEPNLSLMYRLEKYSDRIIPAIYWWKLMIRKQEFLISED
                     QNQRLIEGRRVKNYLEFSG"
     gene            complement(720..1190)
                     /locus_tag="PK198_13280"
     CDS             complement(720..1190)
                     /locus_tag="PK198_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39756.1"
                     /translation="MKILHTATGEYKDAEVVPVAEKDYDYIKNCGRFVFDWRTENRLG
                     FEVYKIALKEYEDHILGLLSLRDIQQELRIHVNLVEVSSDNIGAGKIYDRIAGSLLGF
                     ASQMAFEKSYDGFVSLIPKTDLIDHYCQKYGFSRVGKSLAVEGAGSLAIIQNYL"
     gene            complement(1380..3182)
                     /gene="uvrC"
                     /locus_tag="PK198_13285"
     CDS             complement(1380..3182)
                     /gene="uvrC"
                     /locus_tag="PK198_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRF39757.1"
                     /translation="MTTEDFRAIQDTVPKDPGVYKFIDEEDRILYVGKAKNLRNRLSS
                     YFGERVDRAYRTRLMVKNALRIEFTIVETEADALLLENSLIKRYQPRYNVRLKDDKSY
                     SYICIKNERFPRVFLTRRVIRDGSTYFGPYTSGARLKIILDLIKQLFPLRTCNFLLSE
                     DNVAAGKFKVCLEYHIKNCFGPCEGLEDETAYNERVSQIKNMLKGHFTAVIAHFKNEM
                     QRHAEELEFEKAQQIKEKLAVFEDYQGKSTVVSATIRDVDVFAIAADDKEAYVNYLKV
                     VNGAIIHTHTQEMVKNLDEDEADLLAFAIPELRDRFQSIAPEIIVPFEVALPEPAPQI
                     TIPKIGDKRKLLDLSEKNVRYHLLQRRKEEASKTKRQTPAERILRTLQQDLHMKELPM
                     HLECFDNSNIQGSNPASACVVFKNARPSKKDYRHFQIKTVEGPNDFASMEEVVLRRYR
                     RLLDEGQPLPQLIIIDGGKGQLSAAMKSIETLGIAERVTVIGIAKKLEEIFFPGDSTP
                     LHINKKSESLKLIQQARNEAHRFSLAYHRNRRSRSFLQTELTTIPGIGTKTAEKLLSH
                     FGSVKKLREATVAQMAEVVGMAAAKKVSAGLGAV"
     gene            complement(3228..4577)
                     /locus_tag="PK198_13290"
     CDS             complement(3228..4577)
                     /locus_tag="PK198_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRF39758.1"
                     /translation="MKREDIIAQVLHYFNLEAEVDDYDKIHESITKDIVFKGTNLWIL
                     IFAIVVASVGLNMNSTAVIIGAMLISPLMGPINGMGYSIATYDFPLFRMALKNFGFAV
                     AASLFTSMMYFIISPISTAHSELLARTSPTIYDVLIAMFGGLAGIVAISSKQKGNVIP
                     GVAIATALMPPLCTAGYGLATGQFAYFFGAFYLFAINTVFIALSSVIISQILKFPIRA
                     SLEAAEKVRVNRAISAVIILMLIPSIYFGYNLVQKERFSENAAKYVQNVSVVEGNYLL
                     KHEINADRKTIDLTYGGASLSDEDKKYIQEKAGDFSLKDATIHFQQGLVIGEIASGKA
                     EVESLKSELSRLNRVIREKDEYIDSTFQVRFTGRQILEEIKPLYPQIVSCAFADTYIF
                     DVSDKPLEVSMVIFGITGDPLKEPDQLKIEAWLRSRLQNDRVRVYFEKKSPGTVPQR"
     gene            4724..7177
                     /gene="thrA"
                     /locus_tag="PK198_13295"
     CDS             4724..7177
                     /gene="thrA"
                     /locus_tag="PK198_13295"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRF39759.1"
                     /translation="MKVLKFGGSSVGTPERIRGLVEILKSYYQQGEKFAVVFSAFSGV
                     TDALIAMGTKAADGDASYLDLYETVRQRHLDAAAELLREGYLQQAQPELETNFEVLKS
                     LLYGIFLVREASPRSMDYVLSFGERNSSFLIAQVMRQAGINAAYLDARKVIKTDNRFG
                     SARVDFETTNEKIVEYFSQNPEIQSITGFVGSSKSGLTTTLGRGGSDYTAAIVGAALD
                     ATVIEIWTDVDGVLTADPRKVKKAFTLDSMTYAEAMEMSHFGAKVIYPPTIQPALAKK
                     IPLVIKNTFNPGFPGTLVSEKADPGTLPVKGLSSINEVALLTLQGSGMVGSVGTAGRL
                     FNALAGNGINIILITQGSSEHSITFAIPPQLAAKARKTVEKEFSAEIQRGWTEPVKVE
                     TGLSVIAVIGENMRYRPGIAGQLFQALGKNGINVVAIAQGSSELNISIVVNKEDEAKA
                     LNTLHEAFFLSDTQDVHLFIIGAGLIGSTLLEQIKQQSAYLREKRAMEIKIVGLANSR
                     KMLFNTDGIALDSWKETLAQSELSSDIGAFVQQMKTLNLPNTIFIDNTSGGQAAAHYE
                     NVLDSSISISTPNKIAASSSFIQYQRLKTIAAKRGVLFEYETNVGAGLPVISVLRDLA
                     DSGDRILRIEGVLSGTLSYIFNTFQKGVRFSQTVRQAMELGYTEPDPRDDLSCLDVRR
                     KLLILARETGMMLEAEDIAIHPLLPEACQKAATVEDFLAELENADDWFESLRTEAETA
                     GKKLRMIAKLDHGKASIGLDSVDIQHPFYGLSGSDNMIVFTTERYKERPLVVRGPGAG
                     AEVTAAGVFAEIIKIANFL"
     gene            7210..8142
                     /locus_tag="PK198_13300"
     CDS             7210..8142
                     /locus_tag="PK198_13300"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRF39760.1"
                     /translation="MKKEVRVFAPASVGNAIVGFDILGLALTKPGDEIVARRSDVPGL
                     RIVSITGDEGKLPYEAEKNTAGFAALSLLRHLGQPDWGIELEIHKRMPFGSGLGSSAA
                     SAVGGAYAVNLLLGSPLSKKELLPFAMHGEQLASGGFHADNVAPSLLGGIILICSNQN
                     LDVHSLPVPDELFAAVVYPEVEVLTKTARSILSPNISLRQHIEQSGFLGGFVTALFRS
                     DYELMARSLRDVIIEPQRAQLIPNFHQVQSAALSAGALGCSISGAGPSMIALCRGEAV
                     AQAAGQAMQAVFSETGIRNELFISAVNPHGAIEL"
     gene            8167..>9158
                     /gene="thrC"
                     /locus_tag="PK198_13305"
     CDS             8167..>9158
                     /gene="thrC"
                     /locus_tag="PK198_13305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRF39761.1"
                     /translation="MVSLKEAVFKGLPEDNGLYMPQHIPTLDQNFIRNLKSFSFQEIA
                     FQVADTLMQGAIPKNDLKKIIAEAVNFPAPVHRLDEQKYVLELFHGPSLAFKDFGARF
                     MAQLMSYFNKGENRQLVILVATSGDTGGAVAAGFYQTPGIEVVILYPSGKVSMLQEKQ
                     LTTLGHNVKALEVQGTFDDCQALVKQAFLDAELSSAIRLSSANSINIARLIPQSFYYF
                     EAYKQVSDGTTPIVFCVPSGNFGNLTAGLIAHRMGLPVHHFIAATNINDVVPQYLHTG
                     QYTPRPSTRTLSNAMDVGNPSNFARMLDFFSKENCSTWNNMTASISGYAYNDSQT"
BASE COUNT         2164 a         2323 c         2304 g         2367 t
ORIGIN      
        1 attcacccac aggcagcttg gtatcagcgt tttttagtac ggtcagtacg cgggtttcat
       61 gttccgctac attcggaaat tgggtaaaaa aggcttcgaa gtgcatcgct attgtttttt
      121 tgtttgatac tttgactgcc cgaatgagga agtcagcgcc tcccgcagcc ctgcacgctc
      181 agccgctgaa ttccaggtaa tttttcaccc gccgaccctc gatcagcctt tgattttgat
      241 cctcgctgat gaggaactct tgtttccgaa tcatcagttt ccaccaataa atagcaggta
      301 tgattcggtc ggagtatttt tccaaacgat acatgaggct taaattaggt tcttcccgtt
      361 cattcaaaat cctgctcaat cgcgtataat gcacgtccag atcatttgcc acctcttttt
      421 tggtcttccg catgatggca atcatttcct tcagcacttc gtcaaatgaa taatcgctgg
      481 aatagacgcc ggattctaaa taccggtgaa cagacagtct gagccgggta agttccgaca
      541 acaagcgctc tgattcagtt cgggtactta gttttttcaa acggtattcc cgaatttcct
      601 gctccgcgcc tgctctttct tcttccgtca aatccgcagg aatcagatag ccatcggcga
      661 tatcttcctg tgccagttgc tcagacaagt gctcatatat ttttttgtat tcactcattt
      721 cataaatagt tttgaatgat ggctaaagaa cccgcgcctt caacggccaa acttttgccc
      781 acccggctga atccatactt ttggcagtaa tgatcaatga gatcggtttt gggtatcaat
      841 gaaacgaagc cgtcataaga tttttcaaaa gccatttggg atgcaaaccc taataaactg
      901 ccggctattc gatcatatat tttacctgct ccaatgttgt ccgatgacac ttcaaccaga
      961 ttgacgtgta ttctcaattc ctgctggatg tctcttaaag acaacaaacc taatatgtga
     1021 tcttcatact ctttgagcgc aattttatac acttcaaacc caaggcgatt ttcggtgcgc
     1081 caatcgaaaa caaacctgcc acaattttta atataatcat agtctttttc ggctaccggc
     1141 acaacctcgg cgtctttata ctcgcctgtt gcagtatgca gaatcttcat aaaccatgat
     1201 tattttacaa aaatagtaaa tatttgtgac atatcccata ataagtcgaa atactgactg
     1261 ctggtaatta gcgcgccagc gcgagattcc atgcccctct gagtgagaag ctgaccggaa
     1321 gttatcctga cttcgctctg ccttccctcc aagtgaggag ctgaccggaa gtcagcgcct
     1381 cacacagccc ccagcccggc gctcaccttc ttcgccgcag ccatgcccac cacctcggcc
     1441 atttgggcta cggtggcttc gcggagtttt ttcaccgaac cgaagtgcga gagcagtttt
     1501 tcggcggttt tggttccgat gccaggaatg gtggtgagtt ctgtttgcag gaaactgcgc
     1561 gagcggcggt tgcggtgata ggcgaggctg aagcggtggg cttcgttgcg ggcctgctgt
     1621 atgagcttga gcgattccga ctttttgttg atgtgcagcg gcgtggagtc gccgggaaag
     1681 aagatttctt ccaatttttt ggctatgccg atcaccgtca cccgctcggc gatgcctaag
     1741 gtttcgatgc ttttcatggc ggcgctgagt tgccctttgc cgccgtctat gatgatgagt
     1801 tggggtaggg gttgcccttc gtcgagcagg cggcgatagc ggcgcagcac cacttcctcc
     1861 atgctggcaa agtcgttggg accttctacc gtcttgatct ggaaatgccg gtagtctttt
     1921 ttggagggcc tggcgttttt gaacaccacg caggccgatg ccggattgct gccctgtatg
     1981 ttggaattgt cgaagcattc gaggtgcatg ggcagttcct tcatgtgtaa gtcttgttgg
     2041 agcgtgcgaa gaatgcgttc ggcgggcgtt tgacgcttgg ttttggaggc ttcttccttg
     2101 cggcgctgca gtaggtggta gcgcacattt ttttccgaga ggtcgagcag tttgcgcttg
     2161 tcgccgattt tgggaatggt gatttgaggg gccggttcgg gcagggctac ctcaaacggc
     2221 acgatgatct ccggggcaat gctctggaat cggtcgcgca gttcggggat ggcaaaagcg
     2281 agcaaatcgg cctcgtcttc gtccaaattt ttcaccattt cctgggtatg ggtgtggatg
     2341 atggcgccat tcaccacctt gagatagttg acgtaggctt ccttgtcgtc ggcggcgatg
     2401 gcaaacacgt ccacgtcgcg gatggtggcg ctcactacgg tggacttgcc ctgatagtct
     2461 tcaaaaacgg ccaatttttc cttgatttgc tgggcttttt caaactccag ttcctcggcg
     2521 tggcgctgca tttcattttt aaaatgcgca atcaccgccg tgaaatgccc tttgagcatg
     2581 tttttgatct gcgatacgcg ctcgttgtag gcggtctcgt cttccaaacc ttcgcaggga
     2641 ccgaagcagt ttttgatgtg gtattccaga cagactttga acttgccggc ggccacgttg
     2701 tcctcgctga gcaggaaatt gcaggtacgc agcggaaaca actgcttgat gagatcgagg
     2761 atgattttga gccgcgcacc ggaggtgtat gggccgaagt aggtggagcc gtctcggatg
     2821 acgcggcggg tgaggaatac gcggggaaag cgttcgtttt tgatgcagat gtagctgtac
     2881 gacttgtcgt ctttgaggcg cacgttgtag cggggctggt agcgcttgat gagcgagttt
     2941 tccagcagca gggcatccgc ttccgtttcc acgatggtga actcgatgcg cagggcattt
     3001 ttcaccatca gccgggtgcg ataggcgcgg tccacgcgtt cgccgaagta cgaggagagc
     3061 cggttgcgca ggtttttggc cttgccgacg tacaaaatgc ggtcttcttc gtctatgaat
     3121 ttatagacgc cggggtcttt gggaaccgta tcctgtatag cgcgaaagtc ttcggtggtc
     3181 atcaatgcag atttttttct gataaaatag cggttccctg ctccaattca tctttgcggc
     3241 accgttcccg gagatttttt ttcaaaatac acccgcaccc ggtcgttttg caacctggaa
     3301 cgcagccagg cttcgatttt gagttgatcg ggttctttga ggggatcgcc cgttattcca
     3361 aaaataacca tgctcacctc taagggttta tccgagacat cgaaaatgta agtatcggca
     3421 aaggcacagg aaacgatttg aggatacagc ggcttgattt cctccaaaat ctgcctgccg
     3481 gtaaatctca cctgaaacgt actgtctatg tactcgtctt tttctctgat gaccctgttg
     3541 aggcggctca gttctgattt gagactttct acctccgctt taccggatgc aatctctccg
     3601 atcaccaatc cctgctggaa atggatggtt gcatccttga gagaaaagtc gcccgctttt
     3661 tcttggatgt attttttatc ttcatcactc aaagaagctc cgccgtaggt cagatcaatg
     3721 gtttttctgt cggcgtttat ttcgtgtttg agcaggtaat tgccctccac cacgctcaca
     3781 ttttgaacat acttggccgc attttctgaa aagcgttctt tttgtaccaa gttataacca
     3841 aaataaatac tgggaatgag catcaatatg atgacggcgg agatggcccg attcacccgc
     3901 actttttctg ccgcctccag cgaagcccgg atagggaatt tcaaaatctg agagatgatg
     3961 accgaagaca gcgcaatgaa caccgtgttg atggcaaaca gataaaacgc tccgaaaaag
     4021 taagcaaact gcccggtagc cagcccatag cccgccgtac acaagggcgg catcaaggcg
     4081 gtagcaatgg cgacgccggg aatgacattg cctttttgct tgctgctgat ggccacaatg
     4141 cctgccaaac cgccgaacat ggctatcaat acgtcgtaaa tggtaggact ggttcgggct
     4201 aagagttcag aatgtgccgt cgaaatggga ctgatgatga aatacatcat ggaggtaaac
     4261 aaactggccg ctacggcaaa accgaaattc ttgagggcca tccgaaacag cggaaaatca
     4321 taagtagcaa tgctgtaacc catgccgttg ataggcccca tgagcggaga aatcaacatg
     4381 gcgccgatga tgacggcggt ggaattcatg ttcagtccca ccgaagccac aacgatggca
     4441 aagatcagaa tccagaggtt ggtgccttta aatacgatgt ctttggtgat gctctcatgt
     4501 attttatcat aatcatctac ttccgcctcc agattgaagt agtgcagtac ctgagcaata
     4561 atgtcctctc ttttcatacg ctcggttttt cttgtctgca aaaatacatt catcctgcca
     4621 ataagccgca gttttcaccc gtagtacgat gaggtcgctc tttgtgcaaa attacgacag
     4681 acaattccct acctttgcgg cttcattttc aatcccatcg aacatgaaag tgttgaaatt
     4741 cggaggctca tctgtcggaa cgcccgaacg catccgcggc ctcgtcgaaa tcctgaaaag
     4801 ctattaccag caaggtgaga agtttgcggt cgttttttcc gcgttttccg gcgtcaccga
     4861 tgccctcatt gctatgggta cgaaggctgc cgacggagat gcttcctatc tggatttgta
     4921 cgaaaccgtg cgccaacgcc atttggacgc ggccgccgaa ctcctccgcg agggctatct
     4981 gcaacaggcg cagccggaat tggaaaccaa tttcgaggtg ctcaaaagcc tgctctacgg
     5041 catcttccta gtgcgggaag cctctccgcg cagcatggat tatgtgctga gtttcggcga
     5101 gcgcaattca tctttcctca tcgcgcaggt gatgcggcag gcaggtatca atgccgctta
     5161 tttggatgcc cggaaagtca tcaaaaccga caatcggttt ggcagcgccc gcgtggactt
     5221 tgagaccacc aacgaaaaaa ttgttgaata cttttctcaa aatcctgaaa ttcaatctat
     5281 tacaggattt gtcggctctt ctaaaagtgg cctcacgacc accctaggcc ggggcggttc
     5341 cgactacacc gcagccattg taggcgccgc tttggacgcc accgttatcg aaatctggac
     5401 ggatgtggac ggcgtgctca ccgccgatcc gcgcaaagtg aaaaaggctt ttaccttgga
     5461 cagcatgact tacgccgagg cgatggaaat gtcgcatttc ggggccaaag tcatttatcc
     5521 gcccaccata cagccggctt tggccaagaa gatacctttg gtcatcaaaa acaccttcaa
     5581 tcccggcttt cccggcacgc tcgtttcaga aaaagccgat ccgggtactt tgccggtcaa
     5641 ggggctttct tccatcaatg aggtagcact gcttacgctt cagggcagcg gcatggtcgg
     5701 ctcggtaggt actgccggca gacttttcaa cgcgctggcg ggcaacggca tcaacatcat
     5761 cctcattacg cagggttcgt ccgaacattc catcaccttc gccattccgc cgcaattggc
     5821 cgccaaggcg cgcaaaacgg tggaaaagga attcagcgcc gagattcaac gcggctggac
     5881 cgaaccggtg aaggttgaga ccgggctttc cgtcattgcc gttatcggcg aaaacatgcg
     5941 ctaccgaccg ggtattgccg ggcaactttt tcaggcgctg ggcaaaaacg gcatcaatgt
     6001 ggtggccata gcgcaaggct cttctgaact caatatctcc atcgtcgtca ataaagaaga
     6061 cgaagccaag gctctgaata ccttgcacga agccttcttt ctttccgata cgcaggatgt
     6121 gcatttgttt attatcggcg ccggattaat cggaagtaca ttgttagaac agataaaaca
     6181 gcaatctgcc tatctccgcg agaaacgggc gatggagatc aaaatagtag gattggccaa
     6241 cagccgcaaa atgcttttca ataccgacgg cattgcgttg gattcctgga aagagacgtt
     6301 ggcgcaatcc gagttgagta gtgatatcgg cgcttttgta cagcaaatga aaacgctgaa
     6361 tctgccgaac accattttta ttgacaatac ttcgggcgga caggcggctg ctcattacga
     6421 aaatgtgctc gactccagca tttccatttc tacgcccaat aaaatagccg cgtcgtcctc
     6481 ttttattcaa taccaacggc tcaaaaccat agcagcgaaa agaggcgtgt tgtttgagta
     6541 cgaaaccaat gtcggcgcgg gtttgcctgt tatttccgta ctccgggatt tggccgacag
     6601 cggcgaccgc attttgcgca ttgagggcgt gctttccggt acgctttcct atatttttaa
     6661 tacgtttcaa aaaggcgtgc gtttcagtca aaccgttcgg caggcgatgg aactcggcta
     6721 cacagaacct gatccacgcg acgacctgag ttgcttagat gtgcgccgaa aattgttgat
     6781 tctggcccgc gaaaccggca tgatgctcga agcagaagac attgccattc atccattact
     6841 tcccgaagcc tgtcaaaaag ctgctactgt ggaagatttt ctggcagaat tggaaaatgc
     6901 tgacgattgg tttgaatcgc tgcggacaga ggcagaaaca gccggcaaaa aattgcgcat
     6961 gattgccaag ttggatcatg gaaaagccag tattggtctc gattctgttg acatccagca
     7021 tccattctac ggactaagcg gcagcgacaa tatgatagta tttaccaccg agcgatacaa
     7081 agagcgcccc ttggtggtac gcgggccagg cgccggtgca gaagtaacgg cagcgggtgt
     7141 ttttgcagaa atcatcaaga ttgccaattt tctttaaaca cgcgcaccgt tttgcaatac
     7201 catgaaataa tgaaaaaaga agttcgcgtc tttgcgcctg catccgtagg caatgccatt
     7261 gtggggtttg atattctcgg cttggcgctg accaagcccg gcgacgagat cgttgcccgc
     7321 cgcagcgatg ttcccggact ccgcattgta agcatcaccg gcgacgaagg taaattgcct
     7381 tatgaagcag aaaaaaatac ggctggtttt gcagcgctca gtttgctgcg gcatctgggg
     7441 cagcccgatt ggggcatcga attggagata cacaagcgca tgccattcgg cagtggtttg
     7501 ggttccagtg cagccagtgc ggtaggaggg gcgtacgccg tcaatttgct attgggcagt
     7561 ccgctttcca aaaaggaatt actgcctttt gccatgcacg gcgagcaatt agcatccggc
     7621 ggttttcatg cagataatgt ggcgcccagc ctgctcggtg gcattatttt gatttgcagc
     7681 aaccaaaact tagacgttca ctcattacct gttcccgacg agctttttgc agccgtagtt
     7741 tatccggagg tagaggtact gaccaaaacc gcaaggagta ttttgtcccc gaatatttcg
     7801 ctccgccaac atattgaaca aagtggattt ctgggaggtt tcgtcacggc cttgtttcgc
     7861 agcgactacg aactgatggc ccgctctctg cgcgatgtca ttatcgagcc gcaacgggca
     7921 caactcattc ccaatttcca tcaggtgcaa tccgctgctt tatctgccgg cgcgctggga
     7981 tgcagtatct caggagccgg gccgtctatg attgccctgt gtaggggaga ggccgttgcc
     8041 caagcggcag gacaggccat gcaggcggta ttttccgaaa caggcatacg caacgaactt
     8101 ttcatctctg ccgtcaatcc gcacggcgcc atcgagcttt aatacagcac ccgcaacaaa
     8161 gaaaagttgg tctcactcaa agaagctgtt ttcaaaggtc tgcccgaaga caacgggctg
     8221 tacatgccgc agcacatccc aactttagat cagaacttca ttcgcaactt gaaatcgttc
     8281 agctttcagg aaatagcatt tcaagtggcc gacacgctga tgcaaggcgc cattcccaaa
     8341 aacgacctca aaaagatcat tgccgaggcc gtaaatttcc cagccccggt gcatcgcttg
     8401 gacgagcaaa aatatgtatt ggagcttttt catggcccgt ctttggcttt caaggatttc
     8461 ggtgcgcggt ttatggcgca attgatgagc tatttcaaca aaggcgaaaa tcgtcaattg
     8521 gtcattctcg tggctacttc cggcgatacg ggcggcgcag tggcggcagg tttttaccag
     8581 acgcccggca ttgaggtggt cattctttat ccttccggca aggtgagcat gttgcaggaa
     8641 aagcaactca ccacattggg acacaatgta aaggcattgg aagtacaggg tactttcgac
     8701 gattgccagg ctttggtcaa gcaggctttt ttagacgccg aactcagttc ggccatacgg
     8761 ctcagttctg ccaattccat caacattgcc agattgatac cacaaagctt ttactatttt
     8821 gaggcataca aacaggtcag cgatgggact acgcctattg ttttttgcgt accgagtggc
     8881 aacttcggca acctcacagc cggactgatt gcacatcgaa tgggcctccc ggtacatcat
     8941 ttcattgctg ccaccaatat caacgatgta gtacctcaat accttcatac cggacagtac
     9001 accccaagac cctccacacg cacgctctcc aatgcaatgg acgtgggcaa tccgtccaat
     9061 ttcgctcgta tgctggactt cttttccaaa gagaattgtt ccacgtggaa caacatgacc
     9121 gcctcgatct ctggctacgc atacaatgac agccaaac
//
LOCUS       DAORLF010000160         9139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4101_length_9139_cov_2.356561, whole genome shotgun sequence.
ACCESSION   DAORLF010000160 DAORLF010000000
VERSION     DAORLF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4101_length_9139_cov_2.356561"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /gene="glgP"
                     /locus_tag="PK198_13310"
     CDS             <1..1556
                     /gene="glgP"
                     /locus_tag="PK198_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRF39762.1"
                     /translation="KAYAGGLGILAGDYMKGAKDHGYPIVGIGIKWKQGYTDQMIDAN
                     GQPYDTYHNYKYDFLEDTGVTVTVEIRKREVLCKVWKLECFGNAPLYLLDTDLPENGE
                     DPWITGQLYGWFGEERIAQEMVLGVGGVRALRALGIEVDVYHFNEGHALFAGFELVRE
                     KVEKGAKYEAALAASKQEIVFTTHTPVVQGNESHPIDRLMYMGANMGMTEEQLEALGG
                     SPFNMTVGALRLSRRSNAVAQLHAVTANDMWKEVEGRSEIVGITNAIHLPTWVDAAMI
                     ETAENGGDLWAAHMKNKRDLVDFVKARTGVELIAENLIIGFSRRAVPYKRSDLIFSQP
                     EIIDPLLRDRKIQIVFSGRAHPLDDTGKGIAANLVAMSKKYPGAVVFLENYGMDIGAA
                     LTRGSDVWLNNPRRPKEASGTSGMKAAMNGVLNLSILDGWWPEACQHGVNGWQFGDGF
                     ESEDETILDNHDRAALYRVLTGEVLPTYYDHPEKWVEMMRNSILSTKDYFAVKRMLEE
                     YNELLYKQQ"
     gene            1686..3122
                     /locus_tag="PK198_13315"
     CDS             1686..3122
                     /locus_tag="PK198_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39763.1"
                     /translation="MKRLFTITFCFAFLAMAVHLNAQQLISSQYKGMVSQAQLALQYG
                     PLMQNGVRMYKITYTTPDVFGQQDTASGLLVVPIRPGFTLPLLCYQHGTLDGPNDAPS
                     ALAGGYQLALVFGGLGYVTAAPDLLGIGESRGFHPYVHAESTASASLDMLRATKQYAA
                     ENALSLNNQLFITGYSQGGYSAMALYKDIQENHAAEFTVTAAAPMSGPYSISGVMRDA
                     ILSDQVYLYPAYVPNTLLSYNYVYNLYSSTQQFLKEPYATMAQNYFDRQITLSQLNGM
                     LIQQLTTDFGASIPRNMLQDSIVAAVIANPNHPVNVALRDNDVHDWAPQAPTRLFYCR
                     ADDQVNYLNSVVADSMMQLNGAPNVQALDVNTNADHGGCVQPAVFNTAFFFGQYQSIT
                     VTTSTTATPVLENVRLSPNPAGSVFRVENAPIGAMLEIFDHSGRRLKSQSIQNTMQPV
                     ETNGISRGVYFVCIRSEQGNWYGKVLIQ"
     gene            3206..>3307
                     /locus_tag="PK198_13320"
     CDS             3206..>3307
                     /locus_tag="PK198_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRF39764.1"
                     /translation="MNSQIRGFEGCRHLELWRSRADENVFFTMSIWDS"
     assembly_gap    3308..3335
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3482..4558
                     /locus_tag="PK198_13325"
     CDS             3482..4558
                     /locus_tag="PK198_13325"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF39765.1"
                     /translation="MRISLLIIALSLFSIVSASAQTKADTTIYAVVDEMPRFPGCENP
                     DSSLEARQRCSQQRLLEFVYGNIEYPLEARQKGHEGTAVIAFVVEPDGSISNTTIVKN
                     VEGGCGDEAVRAVGLMNEAGLKWVPGKKAGKPVRVKFNLPVKFNLEEAKPYIMMGYDT
                     VYTELDTPPAFKAGDQALADYLGKAVKYPASGVDSCLLGEIDIQLLVYPNGDVRILNM
                     TDYNDMGYDFWFQAAFAAAGTMGKWEAASFDGRKVSSAYDMTITFAPAAASCQTRVAS
                     YAKAKKLAEEGEQLYNAGDQEGGMAKLNEAVALFPDNAAFLFMRGQAYLNQNKFSEAC
                     MDLFKGRQIAMVTWFDNILRVICK"
     gene            complement(4631..5869)
                     /locus_tag="PK198_13330"
     CDS             complement(4631..5869)
                     /locus_tag="PK198_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39766.1"
                     /translation="MTLTENVRLALRSVRSNMLRAVLTLLIIAFGIMALVGILTAIDS
                     AIYSLNNNLSYLGANTFEIEPGGDRARGNRGGRQDKMDEPVSYRQAMNFKDRFDFPAK
                     SSISMWCSNSAAIRFGEEKTNPNVSIFGVDENYLDGKGFELELGRNFSPKEALEGGYY
                     ALLGSDVVKAVFKGRAEKAIGQVISAGNIKLKVVGVLKSKGSSMNQSEDRRILIPLQT
                     AKRYYGTANTNYRILVAVDDPNQIDHAVSWATAMFRNVRGLKAYEDNDFEISKSDSLI
                     GIIKDNTRYFRYAAAGIGIITLLGAAIGLMNIMLVTVTERTREIGICKAIGATRQGIL
                     VQFLTEAVVISLLGGLIGIFLGVLVGNLVTLLMGGNFLLPWGWIFAAVVTCTIVGLVS
                     GLYPALKAARLDPIESLRYE"
     gene            complement(6036..6971)
                     /locus_tag="PK198_13335"
     CDS             complement(6036..6971)
                     /locus_tag="PK198_13335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRF39767.1"
                     /translation="MSDIKLFSGVSSRHLAERIAFYFGKELGNIQVNRFSDGEMQPVI
                     NESVRGDFVFFIQSTIPPSDNLMELLLMIDAARRASAGYITAVIPYFGYARQDRKDKP
                     RVPISAKLIATLLEAAGANRVMTMDLHADQIQGFFDIPVDHLRSEAIYMPYLKEQNLG
                     NVIFASPDVGGVKRARTYSKYFKTELVICDKYREKANQVAGMTVIGDVSGADVILVDD
                     LVDTAGTLCKAAQAIMDKGANSVRALCTHPVLSGNAYENIENSVLTELMVCDTIPMRQ
                     TSPKINVLSTAMLFSRAIRNMHEHRSISALFLEGL"
     gene            7178..7999
                     /locus_tag="PK198_13340"
     CDS             7178..7999
                     /locus_tag="PK198_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRF39768.1"
                     /translation="MSKPIPYVPGTFSDMLHKLAAMAKPTRKNLWGKIGLAVIIAYIF
                     LCKDISIDLEFTNDAAQSAQHIAASAGNAFSDAPRAVNTSLKEEPARKKAPKKQKTDS
                     NAANTFSNMPFDGELDSEAKKAEKIKKMKAYVKQYAKYAQAEMAKHGIPASITLAQGL
                     LESDAGESRLAVDNNNHFGIKCFSRTCKRGHCSNYTDDTHKDFFRKYKSPSESFKAHS
                     TLLQGSRYKFLYQYRDDDYVSWAKGLKTAGYATDPNYAGKLINLIRELDLHDYDR"
     gene            8037..>9139
                     /locus_tag="PK198_13345"
     CDS             8037..>9139
                     /locus_tag="PK198_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39769.1"
                     /translation="MAKSAHDYIYYGTEAHAGEVRRFVEHVIRANIENERSGGRRTPV
                     CIWGTHGIGKTELVRAIAEENKYDFAYIAPAQFEEMGDLIGMPAIVGDHTVFRAPDWA
                     PKGEGPGILLIDDVNRADDRILRGIMQLLQNFELVSWKLPPFWQIILTANPDGGDYSV
                     TPMDDAMLTRMMHVTMRFDVKVWALWAEQSGVDPRGINFVLTYPELASGRRSTPRSLV
                     QFFEAIKLIPNLKDELALVQMLADSCLDDETAVSFLTYVQHNLSELIAPEEILKADNF
                     KTEVQQRIERTVRQDIFRVDIMATLCTRLVNFLNVRNSKPSDAEVANIRSFIKMPFLP
                     NDLRLAMLQDIIQSPNPSLKTVLADPEISVILL"
BASE COUNT         2266 a         2407 c         2360 g         2078 t
ORIGIN      
        1 tcaaggcgta tgccggcggt ctgggcattt tggccggcga ctacatgaaa ggcgccaaag
       61 atcacggcta ccccatcgtg ggcatcggta tcaaatggaa acagggctac accgatcaga
      121 tgattgacgc caatggtcag ccttacgata cttaccataa ttataagtat gattttctgg
      181 aagataccgg cgtaaccgtg acggtggaaa tccgcaagcg cgaggtactc tgcaaggtct
      241 ggaaactcga atgtttcggc aatgcgcccc tctacctgtt agataccgac ctgcccgaaa
      301 acggcgaaga cccctggatc accgggcaac tctacggctg gttcggcgag gagcgcatcg
      361 cgcaggaaat ggtgctgggc gtaggcggcg tgcgtgcgct gcgggcatta ggcattgagg
      421 tggatgtgta tcacttcaac gaaggccatg ctttgtttgc cggctttgaa ttggtgcggg
      481 aaaaagtgga aaaaggcgca aaatatgagg ccgcattggc tgccagcaaa caggaaatcg
      541 tgttcaccac gcatacgccg gtggtgcagg gcaacgaatc gcaccccata gaccgcctca
      601 tgtacatggg tgccaacatg ggcatgacgg aggagcaatt ggaagcgctg ggcggctctc
      661 cgttcaacat gacggtgggc gcactgcgac tctcgcgccg ttccaatgcg gtggcacaac
      721 tgcacgccgt gaccgccaac gacatgtgga aagaggtaga aggccgctcc gaaatcgtgg
      781 gcatcaccaa cgccattcac ctgcccacct gggtagatgc ggccatgatc gaaacggccg
      841 aaaacggcgg cgacctctgg gctgcgcaca tgaaaaacaa gcgagatttg gtggactttg
      901 tcaaagcccg caccggcgtg gaactcattg ccgaaaacct catcatcgga ttctcgcgcc
      961 gggcagtgcc gtacaaacgc agcgacctca tcttcagtca gcccgaaatc attgacccgc
     1021 tgctgcgcga ccgcaaaatc cagatcgtct tctccggccg cgcccacccg ctcgacgaca
     1081 ccggaaaagg catcgccgcc aacctcgtgg ccatgtcgaa aaaatatccc ggcgccgtgg
     1141 tgttcctcga aaactacggc atggacatcg gcgcagcgct cacccgcggc tccgacgtgt
     1201 ggctcaacaa tccgcgccgc cccaaagaag ccagcggaac cagcggcatg aaggcggcca
     1261 tgaacggcgt gctcaacctc agcatcctcg acggttggtg gcccgaagcc tgccaacacg
     1321 gcgtcaacgg ctggcaattc ggcgatggtt ttgaaagcga agacgaaacg atcctcgaca
     1381 accacgatcg cgccgcgctg taccgggtac tcaccgggga agtgttgccc acctactacg
     1441 atcatcccga aaaatgggtg gaaatgatgc gcaacagcat cctgagcacc aaagactact
     1501 tcgcggtgaa gcgcatgttg gaggagtaca atgagttgct gtataagcag cagtgagata
     1561 aagactgatt gttttaaaaa aatgcggcct tgttgttttt ccaacatggc cgcatttttc
     1621 atttctgata aaccgtattg gaattatctc gttttctttg cattctttca cctaaccata
     1681 cgtccatgaa gcgacttttt accatcactt tttgctttgc atttttggca atggccgtgc
     1741 atctcaatgc ccagcaactc atcagcagcc agtacaaagg catggtttca caggcccaac
     1801 tggcgctcca atacggcccc ctgatgcaga acggggtcag aatgtacaaa atcacttaca
     1861 ccacgcccga tgttttcgga cagcaagaca cggcttccgg tttgctcgtt gttcccattc
     1921 ggcccggatt caccctgccc ctgttgtgtt atcagcacgg cactttggac ggccccaatg
     1981 acgcgccctc tgctctggcc ggcggctatc agttggcctt agtctttggc ggtttgggct
     2041 atgtcactgc cgcgcccgac ttgctgggca tcggcgagtc gcgcgggttt catccgtatg
     2101 tgcatgccga gtccaccgct tcggcttcgt tggatatgtt gcgcgcgacg aaacaatacg
     2161 ctgctgaaaa cgccctttcg ctcaacaacc agctcttcat caccggctat tcgcagggcg
     2221 gctactcggc tatggcttta tacaaagata ttcaggaaaa ccacgccgct gaattcaccg
     2281 tcaccgccgc cgcgcccatg tctgggccgt atagtatttc aggggtgatg cgagacgcca
     2341 ttctgtctga tcaggtgtat ttgtatcctg cctatgtgcc caacacgctg ctgtcgtaca
     2401 actatgtgta taatctttac agctctacgc agcaattcct caaagagccg tatgccacca
     2461 tggctcaaaa ctacttcgac cggcagatca cgctttcgca actgaacggc atgttgatac
     2521 agcaactcac cacagatttc ggtgcttcca ttccgcgcaa tatgttgcaa gacagcattg
     2581 tagcggcggt cattgccaat cccaaccatc cggtcaatgt ggccctgcgc gataatgacg
     2641 tacacgactg ggcgccgcaa gctcccaccc gcctgtttta ttgccgcgcc gacgatcagg
     2701 tgaattacct caacagcgtc gtggccgact ccatgatgca actcaacggc gctcccaatg
     2761 tgcaggcttt ggacgtgaat accaatgccg atcacggcgg ctgtgtgcaa cccgctgtat
     2821 tcaataccgc atttttcttt ggccaatatc agagcatcac cgtaacgacc tctacaactg
     2881 caacacccgt tttggaaaat gtcaggcttt cgcccaatcc ggcggggtcc gttttcagag
     2941 tggaaaatgc tcccatcggc gcaatgctgg aaattttcga tcacagcggt cgccgactga
     3001 aaagccaaag catccaaaac accatgcaac cagtggaaac caacggcatc tccagaggcg
     3061 tatattttgt gtgtatccgc tcggagcagg gcaactggta cgggaaagtg ttgattcaat
     3121 aagttaaact gtcgtgatca aacgcattgt aaaactaact tttgcacccg acaaagctgc
     3181 cgattttctg gtcatctttg aagaaatgaa cagccagatt cgcggctttg aggggtgccg
     3241 gcatctcgaa ctgtggcgca gccgggccga tgagaacgtg tttttcacca tgagcatctg
     3301 ggattcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnggacg agtaccgcca gtcggaggtc
     3361 tttgtacgca cctggcaacg caccaaagcg ctctttgccg cccgcgccga agcttggagc
     3421 gtggatgtta ttgcttaata tgctactttt gcgccctgaa tagaatcaat catccgtact
     3481 tatgagaatc tctcttttga ttattgccct cagcttgttt tccatcgtat ccgcttcggc
     3541 ccaaaccaaa gccgatacca ctatttatgc ggttgtagat gaaatgccgc gtttcccggg
     3601 ctgcgaaaat cccgacagca gcttagaggc ccgccagcgt tgttcgcagc agcgcttgct
     3661 ggagttcgtt tacggcaaca ttgagtaccc gctcgaagcc cgccaaaaag gccacgaagg
     3721 cacggccgtc attgcttttg tggtagaacc cgacggcagt atttccaaca caaccatcgt
     3781 gaaaaacgtg gaaggcggct gcggagacga ggctgttcgg gcagtaggac tcatgaacga
     3841 ggccgggctg aaatgggtgc cgggtaagaa ggccggaaaa ccggtacggg tgaaatttaa
     3901 cttgccggtc aagttcaatc tcgaagaagc caagccctac atcatgatgg gctacgatac
     3961 cgtttacacc gaactcgaca cgcccccggc cttcaaagcg ggcgatcagg cactcgcgga
     4021 ttatttgggc aaagctgtaa aataccccgc ctccggagtg gatagctgcc tgttgggcga
     4081 gatagacatt caactcttgg tgtatcccaa tggcgatgta cgcatcctca acatgacaga
     4141 ctacaacgac atgggctacg atttctggtt tcaagcagct tttgccgccg ccggcacaat
     4201 gggcaaatgg gaggccgctt ccttcgatgg ccgaaaggtg tcttccgctt acgatatgac
     4261 catcaccttt gctccggccg ctgcttcctg ccaaacgcgg gtagcatctt acgcgaaagc
     4321 gaaaaaattg gcagaggaag gcgagcaatt gtacaacgcc ggcgatcagg aaggaggcat
     4381 ggccaaattg aacgaagcgg tcgcgctatt tcccgacaat gctgcatttc tgttcatgcg
     4441 tggtcaggca tatctgaacc aaaacaaatt ctcagaagcc tgtatggatt tattcaaagg
     4501 ccgccaaatt gcgatggtta cctggtttga caatatactg agggtcattt gtaagtgagg
     4561 gcttaaagtt tagtgaaggg gtgattttca tggcattaac agaggaatca ccccttcact
     4621 atttaagtta ttactcgtac ctcaacgatt caatcgggtc taatcgtgca gctttgagtg
     4681 ccggatacaa gccggatacc aaaccgacaa tggtacaggt gactacggct gcgaatatcc
     4741 agccccaggg caggagaaaa ttgcccccca tgagcaacgt caccaaattg cccaccaaca
     4801 cgccgaggaa aatgccgatc aagccgccca gcagagagat aacaacggct tcggtgagaa
     4861 actgaaccaa aatgccttga cgagttgctc cgatggcttt acaaatgccg atctcgcggg
     4921 tacgctccgt caccgtcacc aacatgatgt tcatcaatcc gatggcggcg cccagcagcg
     4981 taatgatgcc gataccagcg gctgcatatc tgaaataacg ggtattgtct ttgatgatgc
     5041 cgatgaggct gtcgcttttg gaaatttcaa aatcgttgtc ctcataagcc ttgagtcctc
     5101 ttacatttct gaacatggcc gtagcccagg acacggcatg gtctatctga ttgggatcgt
     5161 ccaccgctac cagaatgcgg taattggtgt tggccgtgcc gtagtagcgt ttggcagttt
     5221 ggagcggaat gaggatgcgg cggtcttcgc tttggttcat actggagcct ttgcttttca
     5281 gcacgcccac cactttcagt tttatattgc ccgccgaaat cacctgcccg atggcttttt
     5341 cagcccggcc tttaaaaaca gctttcacca cgtcgctgcc cagcagcgca taatagccgc
     5401 cctccagcgc ttctttggga gagaaattcc ggcccagttc caactcgaat ccttttccgt
     5461 ccagatagtt ttcatccacc ccaaaaatgg acacattggg gttggttttt tcctctccga
     5521 atctgatggc ggcagagttg ctgcaccaca tggaaatgga acttttggca ggaaagtcga
     5581 agcgatcttt aaaattcatg gcctgccgat aggagaccgg ttcgtccatc ttatcctgac
     5641 gtccgccccg gttgccgcgt gcgcgatctc cgccgggttc aatttcaaat gtgttggcgc
     5701 cgaggtagga aagattgttg ttcagagaat aaatagcact gtcaatggcg gtcaaaattc
     5761 cgacaagggc catgatgccg aatgcaatga taagcagcgt gagcacggcc ctgagcatat
     5821 tggaacgcac agacctgagc gccaaacgaa cgttttccgt cagtgtcata acgaaatgtt
     5881 ttacctgtga aaagtcaagg aagctgaatc cttgacccaa ctgttgccct gcaggcaatg
     5941 atggatgcag tacagagcag gccgaaggta agtcaactac gcgctgccct gcttttttgt
     6001 tcgatcaaca cacagcgtcg gcagacagaa ccccgtcaaa gcccttccag aaaaagggca
     6061 gagatagacc tgtgttcgtg catgttgcga atggcccgcg aaaaaagcat ggccgtactg
     6121 agcacgttga ttttgggcga ggtttgtctc atggggatgg tgtcgcaaac catgagttcc
     6181 gtgaggacag aattttcgat attttcatag gcattgcccg acaaaaccgg gtgcgtgcag
     6241 agcgcccgca cgctgttggc gcctttgtcc atgatggcct gtgccgcctt gcacaaggtg
     6301 ccggctgtgt ccaccaaatc atccaccaaa atcacatccg cccccgacac atcgccaatg
     6361 accgtcatac ccgccacttg attggctttc tcgcggtatt tatcgcaaat gaccaactcg
     6421 gttttgaaat atttggagta agtacgggcg cgcttcactc cccccacatc cggagaggcg
     6481 aagatgacat tgcccagatt ttgctccttg aggtaaggca tataaatggc ctcgctgcgc
     6541 aggtggtcaa caggaatatc aaagaatccc tgtatttggt cggcgtgcag gtccatcgtc
     6601 atcacgcggt tggcgccggc ggcttccaaa agggtggcaa tgagtttggc ggaaatcggc
     6661 acgcggggct tgtcttttct gtcctgacgg gcatatccga aatagggaat caccgcggtg
     6721 atatagcccg ccgaagctct ccgggcagcg tcaatcatca gcagtaattc catcaggttg
     6781 tcggatggcg gaatggtgga ctggatgaaa aaaacgaaat cgcccctgac agattcattg
     6841 atcacaggct gcatctcgcc gtcgctgaaa cggttgacct gaatgtttcc caattctttg
     6901 ccaaaataaa aggcaatgcg ttcagccaaa tgtcgggaag aaacgccgga aaagagttta
     6961 atatcgctca tcagttgttg gaaggttgtt tctgatgggc cacaaaggta gcaaacttat
     7021 tgcggggagt tcttgtcgga ggttttttat tggaaaagaa aatcgagggg tgaggaatgt
     7081 gtatttttaa ataaaatttt tgttttaatt tgtttttaaa aaacaatttg ttttatattt
     7141 gtgccatcat tgtttgaacc aataaaaaac agaagtcatg agcaagccga taccttatgt
     7201 gcccggtact ttttcagaca tgttgcacaa actggcagca atggcaaagc cgacgaggaa
     7261 aaatctctgg ggaaaaatag gcctggcggt catcatcgcc tacatctttc tttgcaaaga
     7321 catcagcata gatttggaat tcaccaacga tgccgcacaa tcggcgcaac acatcgctgc
     7381 atctgccggc aatgcgtttt ccgatgctcc cagagccgtg aacacctcgc tgaaagaaga
     7441 acccgcccgt aaaaaagctc cgaaaaagca gaaaaccgat tctaacgcgg ccaacacttt
     7501 ttccaacatg ccttttgacg gcgaattgga ttcggaggct aagaaggcgg aaaagatcaa
     7561 aaaaatgaag gcttatgtga agcaatatgc caaatatgcg caagccgaaa tggccaaaca
     7621 cggcatcccg gccagtatca cgctggcgca gggtttactg gaaagcgacg ccggtgaaag
     7681 ccgcttagca gtggacaaca acaatcattt cggtatcaaa tgcttttccc ggacttgcaa
     7741 gagaggccat tgttccaact atacagacga cacgcacaag gattttttca gaaaatacaa
     7801 atcgccctca gagagcttca aggcgcacag tactttactg caaggttcca gatacaagtt
     7861 tttgtaccaa tacagagacg acgattatgt ttcctgggca aaggggctga aaactgccgg
     7921 ctatgccacc gaccccaact acgccggcaa actcatcaat ctgattcgcg aattggacct
     7981 gcacgactac gaccgttgat gtatctttgc aattgcaaca tcaacattca ggcagcatgg
     8041 cgaaatcagc gcacgattac atctactacg gcaccgaggc ccatgcaggg gaggtcagga
     8101 gatttgtaga acacgtcatt cgtgccaata tagaaaatga acgatcaggc gggcgccgaa
     8161 cacccgtttg catctggggg acgcacggaa tcgggaaaac ggagttggtc agagccatcg
     8221 ccgaggagaa caagtatgac tttgcctaca tcgcgccggc gcagtttgaa gaaatgggcg
     8281 acctcatcgg aatgcctgcc atagtgggcg accatacggt tttcagagcg ccggattggg
     8341 cgccgaaggg cgaggggccg ggcattttgc tcattgacga cgtgaaccgg gccgatgatc
     8401 gcattttgcg gggcatcatg cagttgttgc aaaacttcga gttggtgagt tggaaactgc
     8461 ccccgttctg gcaaatcata ttgactgcca acccggatgg cggcgactac tccgtgacgc
     8521 cgatggacga tgccatgctc acccgcatga tgcacgtcac catgaggttt gatgtaaagg
     8581 tctgggcgct ttgggccgaa caaagcggcg tggatccacg cggcatcaat tttgtgctca
     8641 cttatccgga gctggcttcc ggtcggcgca gtacgccgcg ttcattggtg cagttttttg
     8701 aggccatcaa gctgattccc aatctgaaag acgaattggc cttagtgcaa atgctggccg
     8761 attcgtgttt ggatgatgaa actgcggtct cttttttgac ttatgttcag cacaatctga
     8821 gcgaactgat tgcgccggag gaaatcctga aggccgataa ttttaaaacg gaagtgcaac
     8881 agcgcattga acgaaccgtc aggcaagaca tcttccgggt ggacattatg gcaaccttgt
     8941 gtacccgatt ggttaacttt ctcaacgttc gaaattctaa gccgagtgat gccgaggtgg
     9001 ccaatatccg ctcttttatc aaaatgccgt ttttgcccaa cgaccttcga ttggccatgc
     9061 tgcaagacat cattcaatct cccaatccaa gcctgaaaac cgtgttggcc gaccctgaaa
     9121 tcagcgtgat cttgttgaa
//
LOCUS       DAORLF010000161         9135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4103_length_9135_cov_2.309361, whole genome shotgun sequence.
ACCESSION   DAORLF010000161 DAORLF010000000
VERSION     DAORLF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4103_length_9135_cov_2.309361"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..626
                     /locus_tag="PK198_13350"
     CDS             72..626
                     /locus_tag="PK198_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRF39770.1"
                     /translation="MPSALVFTRTKHGADRVAKDLVRAGIKAEAIHGNKSQNARQRAL
                     SNFKSGATRILVATDIAARGIDVEKLSHVINYEIPNIPETYVHRIGRTGRAGADGVAF
                     SFCDTEERAYIRDIHKLTAIQIPVVHDHPFVSEVPVTAPAAAASRPQPQPAQRSSRPA
                     PQGQQPAQKNQQRRKWYGAGRKQA"
     gene            688..834
                     /locus_tag="PK198_13355"
     CDS             688..834
                     /locus_tag="PK198_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39771.1"
                     /translation="MLINSSLGFMEVSGRQNLDMSMANFVRVPLRLVTQVAWRKLFGG
                     KSKA"
     gene            complement(978..1526)
                     /locus_tag="PK198_13360"
     CDS             complement(978..1526)
                     /locus_tag="PK198_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39772.1"
                     /translation="MTRSQFLLLTGIILLSLQCRKNDGLVPECTDCNLTCIAEGEQDV
                     FSNTCRNNYTCTFKWQENAQLDYSANTSVQINPGDKLVFTVRIETDGQEGIADDEFTD
                     LFYFEIDPSMDSFSVESGELNLISARYRQVCFCGDTGLKIPEGCIQGQRIDADHWQIQ
                     ANLTIEYPSWTKALKIDAVFVE"
     gene            complement(1592..1744)
                     /locus_tag="PK198_13365"
     CDS             complement(1592..1744)
                     /locus_tag="PK198_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39773.1"
                     /translation="MTRHARRLGFEFAWQARFHDHIFRNDPEYQRINDYIESNPENWD
                     KDRFYK"
     gene            1905..>4452
                     /locus_tag="PK198_13370"
     CDS             1905..>4452
                     /locus_tag="PK198_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF39774.1"
                     /translation="MEKTIAYQPAHKIRIVTAASLFDGHDAAINIMRRIMQAGGAEII
                     HLGHNRSVEEIVNTAIQEDAQGIAITSYQGGHNEFFKYMYDLLRERGAGHIKIFGGGG
                     GTILPSEIEDLHRYGINRIYSPDDGRRMGLQGMIDDVLRQCDFVLGEHLNGEVKNFDA
                     ARPLSIARLITAVENFPDAASEWLRPVQEQAELATVPVLGITGTGGAGKSSLVDELVR
                     RFLLEFEDKRIAVLSVDPSKRKTGGALLGDRIRMNTASHPRVYMRSMATRQSNLALSR
                     HVQDALHILKAARFDLIILETSGIGQSDTEIVDHSDVSLYVMTPEYGAATQLEKIDML
                     DFADIIAINKFDKRGALDALRDVKKQYQRNHQLWEMPSEDMPVFGTIASQFNDPGMNA
                     LYRKVMQQLAHATGAPLRSATPESKELSEKIYIIPPARTRYLAEIAENNRRYDSWAEH
                     QAAIARRLFGIRQSIEAIESQAIDNKEVILAGLRKAYSDLEKDLDGNCLRGLEGWEDK
                     KAAYAADEYVFQVRGREIRSKTFTTSLSHTRIPRVALPKFKDWGEILRWTLQENVPGE
                     FPYTAGVFPFKRKEEDPTRMFAGEGGPERTNRRFHYVSLDMPANRLSTAFDSVTLYGE
                     DPDHRPDIYGKVGNSGVSICCLDDAKKLYSGFDLCDPRTSVSMTINGPAATICAFFMN
                     AAIDQQCERYIRENGLEHLVETRLKECYDDRGLSRPAYRGELPKGNDGLGLLLLGLTG
                     DQVLPPDVYEQCKARALQAVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDVQEYFIQ
                     HKVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEYYLSRGMKIDDF"
     assembly_gap    4453..4462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4463..5293
                     /locus_tag="PK198_13375"
     CDS             <4463..5293
                     /locus_tag="PK198_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF39775.1"
                     /translation="PNLSFFFSNGVDPEYAVIGRVARRLWAKAMKHKYGANERSQMLK
                     YHIQTSGRSLHAQEISFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAM
                     AIQLIINHELGLAKNENPLQGAFIIEELTDLVEEAVLAEFDRITERGGVLGAMETMYQ
                     RSKIQEESLYYETLKHNGQYPIIGVNTFLGKDGSPTILPGEVIRATDEEKEDQIATLR
                     ALHQAHADKKGSILRNLQQTAIQNGNTFEALMEAVKFCSLGQITNALYEVGGQYRRNM
                     "
     gene            5370..6617
                     /locus_tag="PK198_13380"
     CDS             5370..6617
                     /locus_tag="PK198_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF39776.1"
                     /translation="MKPLLFLLCLLPMFVSAQKNTLSKILDRSATIAPVLREQEKYRV
                     QIVYTQIDRDAANRPAFSTYTYGMAADRYFYPASTVKMPAALLALEKMNKVQVLGLDR
                     NCVMISGAGTPPQTPVTQDETSATGLPSMAHYIRKLFIVSDNDAFNRLYEYLGQEYLT
                     RRLWEKGYTDARILHRLSAPGFDAETNRYTNPVSFYEPPKSGQSWEQSKLLFHQGEVY
                     SRVKSTLKLRDEQQGKGYINSAGEQVNEPFDFSGRNYISLRHLHDIVQAVMFPEDVAA
                     ERRFRLSADDQKFVWKCMSELPRESDYPKYPEKDYPDGYVKFLMFGNSKERIPDHIRI
                     FNKVGNAYGYLTDAAYFVDFNNGVEFLLAATIHINENQIFNDGVYEYDQIGYPFLANL
                     GRAIYEHELKRKKKHAPDLTIYR"
     gene            6765..>7617
                     /locus_tag="PK198_13385"
     CDS             6765..>7617
                     /locus_tag="PK198_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRF39777.1"
                     /translation="MAEDIKKQEALREAALRYHEQGRKGKLEVTATKPFLTQHDLSLA
                     YTPGVAAPCMAIHEDPSAVRRYTAKGNLVAVVTNGTAVLGLGDIGPMAGKPVMEGKAI
                     LFKRFADVDVFDIELGTHDPDEIIRTVQLLEPTFGGINLEDIKAPECFYIEEKLKETM
                     GIPVFHDDQHGTAIISGAALLNAIELTGKKIENIRVVFSGAGAAGIACAKFYIALGVR
                     PENVLMTDSKGVLWKGRGDEGKNKYKDYFYRETEARTLADAMRDADAFCGVSLKDLLT
                     TEMVKTMA"
     assembly_gap    7618..7647
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7648..>8018
                     /locus_tag="PK198_13390"
     CDS             <7648..>8018
                     /locus_tag="PK198_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRF39778.1"
                     /translation="ANPDPEIAYPDAKEARPDAVIATGRSDYPNQVNNVLGFPFIFRG
                     ALDVEAVTINETMKMAAAQALARLAKEEVPEEVKKAYGDYDLTFGPDYIIPKPFDPRV
                     LVWVSSAVAEAAIKTGVARKT"
     assembly_gap    8019..8066
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8067..9068
                     /locus_tag="PK198_13395"
     CDS             <8067..9068
                     /locus_tag="PK198_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRF39779.1"
                     /translation="DWSRELMRKIYISARKDPRRIVFPEGNDHKIIWAASEAVREGIA
                     KPILLSRNKEETLALFEELRHDPSGIEIIVPRHYPHRQEFIEAYHHLRERKGITRISA
                     ENDLRNHFYFGSMMVQQGYADGMVAGATVNYPEVLRPALRVVGTRPGGKLVAGMYLIQ
                     YQNQLYFLSDCAVNITPNAEELAEIAIMTADQVQKMQFEPRIAMLSFSNFGSVRAPET
                     DKVAEAVRIINRLRPDLMADGPIQADTALDMDLLQEHFPFTQLQKRPNVLIFPNLDSA
                     NIGMKLLRKLSSAQNIGPIMVGLDKPIHLVTRDTELNNIVNITAIACVDAQVRNGKK"
BASE COUNT         2224 a         2689 c         2275 g         1859 t
ORIGIN      
        1 accagtcgct cttttatgta gagaaaaacg acaagcgctc gctgctcctg cacctcattc
       61 gcgagcacaa cattccctcc gcgctcgtct tcacgcgcac caaacacggc gcagatcgcg
      121 tggccaaaga cctcgttcgc gcaggcatca aggccgaagc catacacggc aacaagtcgc
      181 aaaacgcccg tcagcgcgcg ttgagcaatt tcaaatcggg cgccacccgc atcctcgtag
      241 ccaccgatat tgccgccaga ggtatagatg tggagaagct ctcgcacgtc atcaactacg
      301 agattcccaa catccccgaa acctacgtcc accgcatcgg gcgcacaggc cgggcaggcg
      361 ccgacggcgt cgcattctct ttctgcgata ccgaagagcg cgcctacatc cgcgacatcc
      421 acaagctcac agccatacag attcccgtcg ttcacgacca cccgtttgta tctgaagtac
      481 ccgtgacggc gcccgcagca gccgcctcgc gcccgcagcc tcaacctgca cagcgcagtt
      541 ccaggcccgc gcctcagggg caacagcccg ctcagaaaaa ccagcagaga agaaagtggt
      601 acggcgccgg cagaaagcag gcataaagta gagttagtta tcgggcaact tggagttgtt
      661 gtccgataac tatacccaaa ccccagtatg ctcatcaact cctccctcgg cttcatggag
      721 gtatcgggcc ggcaaaatct ggacatgtct atggccaatt tcgtgcgggt accgctccgc
      781 ttggtcacgc aggtggcatg gcgcaaactc ttcggcggca aaagcaaagc ataaacagac
      841 cccgaccaga tagacgaaat agatatcatc ggcgatcgtg ataaagtgcg ctacctccat
      901 atccgcatca cctgcccccc gacgactata aagaggggct gggctgggat aagaagtaga
      961 agtagcggct ctcaacctta ttcaacaaat accgcatcta tcttcagcgc tttggtccat
     1021 gagggatatt caatcgtcaa attggcctgt atttgccagt gatcggcatc tatgcgctgc
     1081 ccctgaatgc agccttcagg gattttcaat cccgtgtcgc cacaaaagca aacctggcga
     1141 taccgggcgc tgatcaggtt cagttctcca ctttccacag agaagctatc catggaagga
     1201 tcaatttcaa aatagaataa atcggtaaat tcatcatccg caatcccttc ttggccatcc
     1261 gtttctatcc gaaccgtaaa caccaattta tcacccggat tgatttgaac agacgtattg
     1321 gcgctgtaat ccaattgcgc gttttcctgc catttgaatg tacatgtgta attgtttcta
     1381 caggtattgg aaaaaacgtc ttgttcgcct tcagcgatgc aggtcaggtt gcagtccgtg
     1441 cattcgggca cgaggccgtc gtttttgcgg cattgcaggc tgagcaggat aatgccggtc
     1501 aggaggagga attgtgagcg tgtcataata taaagattgt ttcagccgca tagacgtacc
     1561 attagggatg cccggttgga actgacgggg attatttata aaacctgtcc ttatcccaat
     1621 tttccgggtt ggattctatg taatcgttga tgcgttgata ttcggggtca tttcggaaga
     1681 tatgatcatg gaaacgggct tgccaggcaa attcaaaccc caaacgccgg gcgtgtctgg
     1741 tcacggccga tttgtacgat tctataatgg acgaaacggt atttttacaa aaatacataa
     1801 tcatcccatc tcccccaaac tccctacctt tgtcccgcca tcgttgcgcc aggcccgggc
     1861 cggcgcctgc gtgttaacca agtttaaaac ctagtattac cgccatggaa aagaccattg
     1921 cctaccaacc agcccacaaa atccgcatcg tcaccgccgc ctctctcttc gacggccacg
     1981 atgccgccat caacatcatg cgccgcatca tgcaggccgg aggcgccgaa atcattcacc
     2041 tcggccacaa ccgctcggtg gaagaaatcg tcaacaccgc cattcaggaa gatgcgcaag
     2101 gcatcgccat cacttcctac cagggcggcc acaacgagtt tttcaaatac atgtacgacc
     2161 tgctgcgcga gcgcggcgcc ggccacatca aaatcttcgg cggcggcggc ggcaccatac
     2221 tgccctccga aatcgaagac ctgcaccgct acggcatcaa ccgcatttac tcgcccgacg
     2281 acggccgccg catgggcctt cagggcatga ttgacgacgt gctgcgccag tgcgattttg
     2341 tactcggcga acacctcaac ggcgaggtca aaaactttga cgccgcccgg ccgctctcca
     2401 tcgcgcgcct catcactgcc gtagagaact tccccgatgc cgcatccgag tggctccggc
     2461 ccgttcaaga gcaggccgaa ttggccactg tgcccgtgtt gggcatcacc ggcaccggcg
     2521 gcgcgggcaa gtcaagcttg gtggatgaat tggtgcggcg gttccttttg gaatttgaag
     2581 acaaacgcat cgccgtgctt tcggtggacc cctccaaacg caaaaccggc ggcgcattgc
     2641 tcggcgaccg catccgcatg aacactgcct cgcacccccg cgtatatatg cgcagcatgg
     2701 ccacccggca gtccaacctc gccctgtcgc gccatgtgca ggacgcgctc cacatcctca
     2761 aagccgcccg cttcgacctc atcatcctcg aaacctccgg catcggccag tccgacaccg
     2821 aaatcgtgga tcactccgat gtgtcgctct atgtgatgac gcccgaatac ggggccgcca
     2881 cgcagttgga aaaaattgac atgctcgatt ttgccgacat catcgccatc aacaagttcg
     2941 acaagcgcgg cgccctcgat gccctccgcg atgtgaaaaa gcagtaccag cgcaaccatc
     3001 agctttggga aatgccctcc gaggacatgc ccgtgttcgg cacaatcgcc tcgcagttca
     3061 acgatcccgg catgaacgcc ctctaccgca aggtgatgca gcagttggcc cacgctaccg
     3121 gcgcgccgct gcgttcggcc acgccggaga gcaaggaact gagcgaaaaa atttacatca
     3181 taccgccggc ccgcacccgc tatctggccg aaatagccga aaacaatcgc cgctacgaca
     3241 gttgggccga acatcaggcc gccattgcgc gcagactttt cggcatccga caatctatcg
     3301 aagcgattga aagccaagcg attgataaca aagaagtcat cttggcgggg cttcgaaaag
     3361 catattccga tctggaaaaa gacttggacg gcaattgcct ccggggcctc gaaggctggg
     3421 aggacaaaaa agccgcctac gctgccgatg agtacgtttt tcaggtgcgg ggccgagaaa
     3481 tccgcagcaa aaccttcacc acctcgctat cgcatacccg cattccccgc gtagccctgc
     3541 cgaagttcaa agactggggc gaaatcctgc gctggacttt gcaggaaaac gtgccgggcg
     3601 agtttcccta caccgcgggc gtgttcccct tcaagcgcaa ggaggaagac cccacgcgca
     3661 tgtttgccgg cgagggcggc cccgaacgca ccaaccgccg cttccactat gtatcactcg
     3721 acatgcccgc caaccgcctc agcacggcct tcgactcggt gacgctctac ggcgaagacc
     3781 ccgaccatcg gcccgacatc tatggcaagg tgggcaactc cggcgtgagc atttgctgtc
     3841 tcgacgatgc caaaaagctt tactccggct tcgatctctg cgatccgcgc acctcggtgt
     3901 ccatgaccat caacgggccg gcagctacca tctgcgcctt ttttatgaac gccgccattg
     3961 accagcagtg cgagcgctac atccgcgaaa acggcctcga acatttggtg gaaacccgcc
     4021 tgaaagaatg ctacgacgac cgcggtctgt cccggcctgc ctatcggggc gaactgccca
     4081 aaggcaacga cggcctcggt ctgctcctgc tgggcctcac cggcgatcag gtattgcctc
     4141 ccgatgtgta cgagcagtgc aaagcccgcg ctctccaggc ggtgcgcggc acggttcagg
     4201 ctgatattct caaagaagat caggctcaga acacctgtat tttcagcacc gagttctcgc
     4261 tgcgcctcat gggcgacgtg caggagtatt tcatccaaca caaagtccgc aatttttact
     4321 ccgtctccat ctccggttat cacattgccg aggcaggcgc caatcccatt tcgcaattgg
     4381 ccttcacctt agccaacggc ttcactttcg tggaatacta cctcagccgg ggcatgaaga
     4441 ttgatgattt tgnnnnnnnn nncccaatct ctccttcttt ttctccaacg gcgttgaccc
     4501 cgaatatgcc gtcatcggtc gggtggcgcg ccgcctctgg gccaaggcca tgaagcacaa
     4561 gtacggcgcc aacgagcgca gccaaatgct caagtaccac atccagacca gcggtcgctc
     4621 gctgcacgct caggagattt cgttcaacga catccgcacc accctgcagg cgctctacgc
     4681 catctacgac aactgcaaca gcctgcatac caacgcctac gacgaggcca tcaccacgcc
     4741 cacagaggaa agcgtgcgcc gtgccatggc catccagctc atcatcaacc acgaactggg
     4801 tttggctaaa aacgaaaacc cgctgcaggg cgctttcatc atcgaagagc tgaccgacct
     4861 ggtagaagaa gccgtgctgg ccgaattcga ccgcatcacc gagcgcggcg gcgtgctggg
     4921 cgctatggaa accatgtatc agcgcagcaa aatacaggag gaaagccttt attacgaaac
     4981 gctgaaacac aacggccaat accctatcat cggggtcaat actttcttag gcaaagacgg
     5041 ctcgcccacc atcctgcccg gagaggtcat ccgcgctacc gacgaagaaa aggaggacca
     5101 gatagccacc ctgcgcgcac tgcatcaggc tcatgcagac aaaaaaggct ccattctgcg
     5161 caatttgcaa caaaccgcca tccaaaacgg caataccttc gaggcgctca tggaggcggt
     5221 gaagttttgc tcattgggcc agatcacgaa tgcactgtac gaggtgggcg ggcagtatag
     5281 gaggaatatg tagtggaatt gcagctccat atcgtccgca ccgatgaaaa atatcagctc
     5341 tcttaaagaa atatagatcg tacctgccga tgaaaccact gctctttctg ctctgcctgc
     5401 tgccgatgtt tgtatcggcg caaaaaaata ccctttccaa aatcctcgac cgcagcgcca
     5461 ctatagcacc tgttttgcgc gagcaggaaa aataccgcgt gcaaatcgtt tatacacaaa
     5521 tagaccgcga cgctgccaat cgccctgcat tttctaccta tacctacggc atggccgccg
     5581 accggtattt ttacccggcc agcacggtca aaatgcctgc tgcgctgctg gccttagaaa
     5641 aaatgaacaa agtacaagtg ctgggattgg accgcaactg cgtgatgatt tccggcgccg
     5701 gcacgccgcc ccaaacgcca gtcacccaag atgagacttc ggctaccggg ttgccctcta
     5761 tggcgcatta tattcgtaaa ttgttcattg tcagtgataa tgatgcattc aatcgccttt
     5821 atgagtacct ggggcaggaa tatctcaccc gccggctttg ggaaaaaggc tacaccgatg
     5881 cccggatttt gcaccggctt tctgctcccg gtttcgatgc cgaaaccaat cgttatacca
     5941 atccggtcag tttttatgag ccgccgaagt cgggccaaag ctgggaacag agcaagctcc
     6001 tctttcatca gggcgaggtt tacagcaggg taaaatcaac gctgaaattg cgcgacgaac
     6061 agcaaggcaa aggctacatc aactccgccg gcgagcaggt gaatgagccg tttgatttca
     6121 gcgggcgcaa ttatatttcg ctgcgccatt tgcacgacat cgtgcaggcg gtaatgtttc
     6181 cagaggacgt ggctgccgag cgccggtttc gcctcagtgc cgatgatcaa aagtttgtgt
     6241 ggaagtgcat gtcggagctg cctcgcgaga gtgattatcc aaaatatccc gaaaaggatt
     6301 accccgacgg ctatgtcaaa ttcctgatgt tcggcaactc caaagagcgc attcccgatc
     6361 acattcgcat ttttaacaaa gtgggcaatg cttacggcta ccttaccgac gctgcttatt
     6421 ttgtagattt taataatggt gtggagtttt tactggccgc caccatacac atcaacgaaa
     6481 accagatttt caacgatggg gtatatgagt acgatcaaat cgggtatcct tttttagcca
     6541 atctggggcg ggccatttat gagcacgaat tgaagcgaaa gaaaaaacat gcgcctgact
     6601 tgacgatata tcgttgaaaa aataaaattt gccgcaggat agcgctaact ttgagcgacg
     6661 aaatttgccg catgtctatg gtcgattttt atagttgttc tcagcaatga caaccgaaaa
     6721 gaccacagcc aggcgcacta ccaaataaca accaaacaat caccatggca gaagacatca
     6781 aaaagcagga agccctgcgg gaagcagccc tgcgttacca cgaacaaggc cgcaaaggta
     6841 aattggaagt gacggccacc aagccctttc tcacccagca cgatctcagt ctggcttata
     6901 cgcccggagt agccgcgccc tgtatggcca ttcacgaaga cccctccgcc gtgcggcgct
     6961 ataccgccaa aggtaatttg gtggccgtag ttaccaatgg caccgccgta ctcggcctcg
     7021 gcgacatcgg ccctatggcc ggcaagcccg tgatggaagg caaagccatc ctgttcaagc
     7081 gttttgcaga tgtggacgtg tttgatatag aactcggcac ccacgatccc gacgagatca
     7141 ttcgcaccgt gcaactgctg gagcctactt tcggcggcat caatctggaa gacatcaaag
     7201 cgccggagtg cttctacatc gaagaaaaac tgaaggaaac gatgggcatt cccgtttttc
     7261 acgacgatca gcacggcacg gccatcattt caggagcagc gctgctgaat gccatagagt
     7321 tgacaggcaa aaaaattgaa aatatccgcg tggtcttttc cggcgccggg gcagcgggca
     7381 ttgcctgcgc caagttttat attgcattgg gtgtgcgccc cgaaaacgtg ctgatgaccg
     7441 acagcaaagg cgtactgtgg aaaggccgcg gcgacgaagg caaaaacaaa tacaaagatt
     7501 acttctaccg cgaaaccgag gcccgcacct tagccgatgc catgcgcgac gccgatgctt
     7561 tctgcggtgt gtcgcttaaa gacctgctca ctacggaaat ggtaaaaacc atggcgcnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc aatcccgatc cggaaattgc ctaccctgat
     7681 gccaaagaag cccgcccgga tgccgtcatc gccaccggtc gcagcgacta tcccaatcag
     7741 gtaaacaatg tgctgggctt cccctttatc tttcgcggcg cccttgatgt ggaggccgtc
     7801 accatcaacg aaaccatgaa aatggccgct gcccaggcat tggcccgcct tgctaaagaa
     7861 gaagtgccgg aagaggtgaa gaaggcttat ggagactacg acctcacttt cgggccggat
     7921 tacatcattc ccaaaccctt cgatccgcgt gtcttggttt gggtatcttc ggcagtagcc
     7981 gaggccgcca taaaaaccgg cgtggcccgc aaaaccatnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnngact ggtcgcgcga gctgatgcgc aaaatttaca
     8101 tctcggcccg caaagatccg cgccgcatcg tattccccga aggcaacgac cacaaaatca
     8161 tctgggccgc cagcgaggcc gtgcgcgagg gcattgccaa gcccattctc ctctcccgaa
     8221 acaaagaaga aacgctggct ttgtttgaag agctgcgcca tgatccaagc ggcattgaga
     8281 ttatcgtgcc gaggcattat cctcatcgtc aggaatttat cgaggcatat caccatttgc
     8341 gggagcgcaa aggcatcacc cgtatcagcg ctgaaaacga cctgcgcaac cacttctact
     8401 ttggctccat gatggtgcag caaggctacg ccgatggtat ggtggcaggg gctaccgtca
     8461 actacccgga agtgctgcgg cctgcgctgc gggtggtagg tacgcgtcca ggcggcaagt
     8521 tggtggcagg catgtatctg attcaatatc aaaatcaact gtatttcctc tctgactgcg
     8581 ccgtcaacat cacgcccaat gcagaagaac tggccgaaat tgccattatg actgccgatc
     8641 aggtgcagaa aatgcagttc gagccgcgca ttgccatgct ttcgttttcc aacttcggtt
     8701 cggtacgcgc cccggaaacg gataaagtgg ccgaagccgt cagaatcatc aaccgcctgc
     8761 gccccgacct catggccgac ggccccattc aggccgatac agccttagat atggacttat
     8821 tgcaagaaca ttttccattc acccaattgc agaagcgccc caatgtgctg attttcccta
     8881 acttagactc ggccaacatc ggcatgaagc tgctgcgcaa actcagctcg gcccaaaaca
     8941 tcggccccat tatggtgggc ttagacaagc cgattcactt ggtcacgcgc gatacagaac
     9001 tcaacaatat tgtcaatatt accgctattg cttgtgtgga tgcacaggtg agaaacggga
     9061 aaaagtaatt ttcagagtgt cggggccggg ggcaaccctg cgcggcggcg cttaaatttt
     9121 accttctttg acaat
//
LOCUS       DAORLF010000162         9118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4112_length_9118_cov_2.035750, whole genome shotgun sequence.
ACCESSION   DAORLF010000162 DAORLF010000000
VERSION     DAORLF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_9118_cov_2.035750"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK198_13400"
     CDS             complement(<1..322)
                     /locus_tag="PK198_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39780.1"
                     /translation="MIPPTDDLRPSPERTSSVSVGRSPATLSHIIHTLQNSGPLRPAE
                     LRRLVLEAQVKQEDLLPWADFEHPVADSYGRKLAYKEDHFEIMVMSWLPGDFSSLHDH
                     GFTQY"
     gene            complement(429..1616)
                     /locus_tag="PK198_13405"
     CDS             complement(429..1616)
                     /locus_tag="PK198_13405"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRF39781.1"
                     /translation="MQHPHSNFELIEKQSQLDHFYAVNRQCEWMGLDTEFVGEKRFVT
                     LLCLIQVSTPNGNYIIDPLQLNDLGPFLQLIQDPAILKITHAGENEYRIFYQQYDVVP
                     ANVFDTQLAAAFLGYKHPVSFRRLAENELGLKVDKTLTTADWEARPLNKSYLRYALDD
                     VAPLHALMQQMETKLRKHGRLEWAREEFALLENADYYAKDPNDEALNNSMMKSLNFKE
                     KIFMLRLYQWRREQAEAKNYSKEMILPAKYIAPIVKGISAGLDALRHNRRLPERTIQQ
                     FGRLFVQFYQQPATAEEKNILTRLDLSEEDNDDPMEEIMQEMLYLLVRYKCLESGITP
                     AFVMPKGVLRKLKSNPEDMPENLETGWRRQMLGEDIFLWMKHYERLEMSVASGRIELR
                     VRP"
     gene            1808..>2039
                     /locus_tag="PK198_13410"
     CDS             1808..>2039
                     /locus_tag="PK198_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39782.1"
                     /translation="MKIFKRLLLIAVVLLVLVLGAAAAFPFVFKDEILAAVKREINVN
                     LTAKVDFEDLNLSLFRNFPNVSVGLENYSVTGT"
     assembly_gap    2040..2067
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2068..5033
                     /locus_tag="PK198_13415"
     CDS             <2068..5033
                     /locus_tag="PK198_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRF39783.1"
                     /translation="TLAKGELLDVEVDLSSFWNKDKPMKINSVHLKKPNIKVYVLEDG
                     RANYNIVKTEETIIVDSITGEPIVVEPTPFEINLDGYSISEGKILYQDATNDLYLSIA
                     GLNHKGSGNFTQDVFDLETKTQMDTLTVRYGAIPYVKDAVLDLDAVFQIEMPVSKYTL
                     KDNKLRINDLELQADGFVQMLENDDIGMDIKLSTPQNDFKALLSLIPNAYIQNFEQVK
                     AGGRFEFGAQAKGIYNGDREEYPAFDVKLAVADGSVQYPGLPMGMEQIQAEMNVNSPG
                     GNLDALSVNVPRFGLLVGKRPFDLVFFLKTPLSDPDLDAKMKGVIHLAEWAKAFPMEG
                     VNGGIIDADARIKTRMSYIDNGQYDRVDMSGKVAIQNLKYKSEGLPPVHIQSLSTVFA
                     PQRVEINNFEGKLGESDLKARGSIKNILAYFSPKKTMYGDLAFSSQFFNINEWMPTQE
                     DAEPAPSVVDAAPAGSATPTTTESESASAIFDRFDFTVDGSIREVVYSDYRLLNTYAK
                     GNIKPNRMEIDDSGFLIGDSDMQASGLVLNMFDYLFNNGTLGGNLKINSKLMNLNQFM
                     GEAPAENAAPTPSSNTTTSSSDGSGYTPVMVPANVKMAIDANIGRLIYTDYELSNLDG
                     QLVVEDETVVVRDAVAGLLGGKVGLTGGYDTRDPENPAFNMKYDMQNMSFQRVFKALN
                     TFKMLTPLAGYIEGNFNTALIMEGKLGQDMMPRIASLNVKGFLETLNGVISNFKPFQE
                     LGSMLDVSYLKDNINLTNTKNWFEIQNGIVELKEFDYKYKDIDMKISGTHSLMQDMHY
                     KIKARVPRKLLEKNVAGATASKGYDLLRKEASKFGVNLQKSEFVNMLIDVTGTTTKPK
                     VALHLLGADGETPVDDAVKEELKAEVEKAKAEVKQQVEQKIEEGKQIVKETADKALDT
                     VKTVANQKLDELEKEVKDAAKEKLNEVVGDKIDSTALKKAQEIIDKNKEAERIKKELE
                     KFNPLKKKKEGGGRR"
     gene            5238..5774
                     /locus_tag="PK198_13420"
     CDS             5238..5774
                     /locus_tag="PK198_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF39784.1"
                     /translation="MTEEELARGCIREDSACQQELFRRYAGKMMAVCLRYARSHEEAE
                     DLLQDSFIKVFDGIADFQFKGSLEGWVRRITINTALRHYQRSSLRPEMNGAEWELETA
                     EEPDVYGYLEAESLMNLISKLPDGYRMVFNLYAIEGYNHKEIAEMLGIQESTSRSQLV
                     KARKMLQHWLEEFKIIAV"
     gene            5771..7111
                     /locus_tag="PK198_13425"
     CDS             5771..7111
                     /locus_tag="PK198_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39785.1"
                     /translation="MNRRHPVDDFFKDKLKNHSVEPSAGMWERIEEERRRSAVAVTPA
                     RLSGWHLLAVAFIAVLAPLTPRQTKQSVSNPSEIIMAQQAAPDSEAVAPTGPLAEAPR
                     LQAALQNEPTETGIAYAQDARDVEHHHSAPAFTLHAQRPDIVAHPISVSTHQLVYDAA
                     ATTEAASEESLYTPIAEFEPLPLRRHFALEQERNGSFPAASQCATFKNMGLRFYIEAH
                     ASPDLAFRQISPAGDSPEQAAFAQKRDQTERPFYNYSFGARLAASTRFGLVMRTGFDY
                     AQINERLRYVTETEERFTIINIIGPGGSVIGVDTVYETIVHEHTAQNSYRTLSIPVLA
                     GYELPVKRWLFSAQGGILLNLLFAQQGSMYLPGTDMPVRFSTMEQEGRPAFKSRLGLG
                     WYAGLGAAYKIRHNMYLTAEPHVRAFPGSVTRHDFDTKQSYISGGLALGLKVKL"
     gene            7187..7780
                     /locus_tag="PK198_13430"
     CDS             7187..7780
                     /locus_tag="PK198_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39786.1"
                     /translation="MKIKYRITSCLLFFSVICLHAQSPQDAALIDAASQMNFIDRYRN
                     ETNGTYVYGIPGHAGRNEIPESFFNGVPYFRLNDYSYIQGNYSGKTLNRLNNKYGDTW
                     LTLGNIDADAGTICVKAGWYNGLVGEVHLEAPMSSTNIFSGEGLLYYNGRAMYDIYMF
                     FFMLGDWKTMKGVCVLNPRTSDGVLQTSTFVLKSTSP"
     gene            7820..8092
                     /locus_tag="PK198_13435"
     CDS             7820..8092
                     /locus_tag="PK198_13435"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRF39787.1"
                     /translation="MNDIEMARFDARMTKEQKEFFEYASKLGGFRSLTEFVLSVVQSK
                     AHEIVEEDNKILASKRDQEIFFDLVTKETPPNDALKSAFQSYNSQF"
     gene            8252..>8474
                     /locus_tag="PK198_13440"
     CDS             8252..>8474
                     /locus_tag="PK198_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HRF39788.1"
                     /translation="MSVLKDKYINPLTDFGFKKLFGTEPNKILLIDFLNQILPERHRI
                     TDLSYSRNEHLGLNELDRKAIFDLYCIGKS"
     assembly_gap    8475..8529
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8530..>8869
                     /locus_tag="PK198_13445"
     CDS             <8530..>8869
                     /locus_tag="PK198_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HRF39789.1"
                     /translation="SVYYASFPIQEQAGKGEWDYRLEPVYSVGILDFIFDDHKSDEEL
                     LHTVELKNQRCEVFYDKLKFIYIELPKFKKQEDELETHFDKWLYVFRHLSNLQDRPKK
                     LQDRVFQKLFD"
     assembly_gap    8870..8889
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8890..9107
                     /locus_tag="PK198_13450"
     CDS             <8890..9107
                     /locus_tag="PK198_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39790.1"
                     /translation="AEIAKFSPEERDAYEDSLKYYRDLKNVVDTSREEGMIEGIEKIA
                     KKMKEKGMSNSEISELTGLNDDEINDL"
BASE COUNT         2432 a         2174 c         2315 g         2094 t
ORIGIN      
        1 cgtattgggt gaagccgtga tcgtggaggc tggagaagtc gccgggcagc caggacatga
       61 ccatgatctc gaagtggtct tctttatagg cgagtttgcg accgtagctg tcggctacgg
      121 ggtgctcgaa gtcggcccag ggcaggaggt cttcctgctt cacttgtgct tcaaggacaa
      181 ggcggcggag ttcggcgggg cggagggggc cgctgttttg cagtgtgtgg atgatgtggg
      241 agagcgtcgc cggactgcgt ccgacggaga cagatgacgt cctttcagga cttgggcgga
      301 gatcgtcggt tggtggtatc atgcttttat aaatgcggcg agatacatga ttgttttttc
      361 atgtgttgga ttttggcagt tgaaatctca atgagaatga agcaggtggc cggtttcgac
      421 gggtgctgtt acgggcgtac gcgcagttcg atgcggcccg aagcgacgga catttccaaa
      481 cgttcgtagt gtttcatcca caggaagatg tcttcgccca gcatttgcct gcgccagccg
      541 gtttccaagt tttcgggcat gtcttcgggg ttgcttttga gcttgcgcag cacgcctttc
      601 ggcataacga aggccggtgt gatgccgctt tcgagacatt tgtaacgtac cagcaggtag
      661 agcatttcct gcatgatttc ctccatcgga tcgtcgttgt cttcttcgga gaggtcgagg
      721 cgggtgagga tgtttttttc ttcggcggtg gcgggctgtt ggtaaaattg tacaaacagg
      781 cggccaaatt gctgaatggt gcgttcgggc aggcggcgat tatggcgcag cgcatccagt
      841 ccggctgaaa tacccttgac gatgggcgcg atgtatttgg ccggcagaat catttctttg
      901 gagtagtttt tggcttcggc ctgttcgcgg cgccattggt agaggcgcag catgaagatt
      961 ttttctttaa aattgaggga tttcatcatg ctgttgttga gggcctcgtc gttggggtct
     1021 ttggcgtagt agtcggcatt ttcgagcagg gcaaattcct ctctggccca ttccaagcgc
     1081 ccgtgtttgc gcagtttggt ctccatctgt tgcatgaggg cgtgcagagg cgctacgtcg
     1141 tcgagagcat agcgcaggta gcttttgttg aggggccgcg cttcccagtc ggcggtcgtg
     1201 agggttttgt ccaccttcag gcccagctcg ttttcagcta agcggcgaaa cgatacgggg
     1261 tgtttgtagc ccaaaaaagc ggcggccaat tgcgtgtcga atacattggc cggcaccacg
     1321 tcatattgtt ggtaaaaaat gcggtattca ttctcgccgg catgggtaat tttgagaatg
     1381 gcggggtctt ggatgagttg aagaaacggc cccaaatcat tcagttgcag cgggtctatt
     1441 atataatttc cgttgggcgt ggatacctga atgaggcaaa gaagtgtgac aaatcgtttt
     1501 tcgcccacga attccgtgtc aagccccatc cattcgcact gccggttgac ggcgtaaaaa
     1561 tgatctagtt ggctctgttt ttcaatgagt tcgaagttgg agtgaggatg ttgcatgatg
     1621 tgtttttgtg caaaaaaggc gcctgttcaa aaccaagtat tgcaggtgct gcgtttaaga
     1681 tgatgataaa taaaggttgg catacgagcg gctcttttta tatctttgag caccggcgtt
     1741 tgcaacctga caacaggacc gacgtctcaa ttgtcttaaa tttgatgcaa aactacgaaa
     1801 atcgcacatg aaaatcttta aacgcctact gctgattgca gttgttcttt tggtgctggt
     1861 actgggcgcc gctgccgctt ttccatttgt tttcaaagac gaaattttgg ctgccgtcaa
     1921 acgggaaatc aatgtaaatc tgacggccaa agtggacttt gaagacctca acctctcgct
     1981 ctttcggaat ttccccaacg tgtccgtagg tttggaaaac tactccgtca ccggaacggn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnntca ccttagccaa aggagagcta ttagatgtag
     2101 aggtggattt gtcttccttt tggaataaag acaaacccat gaaaatcaac tctgtccacc
     2161 tcaaaaagcc caacatcaag gtgtatgtgc tggaagacgg cagagccaac tacaacatcg
     2221 tcaaaaccga agaaacgatc atcgtggatt ccattaccgg cgaacccatc gtggtagaac
     2281 ccacgccttt tgaaatcaac ttagacggtt acagtatctc tgaaggcaaa atcctgtatc
     2341 aggatgccac caacgatctc tacctctcca ttgcaggcct caaccataaa ggcagcggca
     2401 atttcacgca ggacgtcttc gatctggaaa ccaaaaccca aatggatacg ctcacggttc
     2461 ggtatggcgc cattccttac gtcaaagacg cagttttaga cttagacgca gtgtttcaga
     2521 tagaaatgcc ggtgtcgaaa tatacgctca aagacaataa actccgcatc aacgacctcg
     2581 aactgcaagc agatggtttt gtacaaatgc ttgaaaacga tgacattgga atggatatta
     2641 aactgagcac cccgcaaaac gatttcaaag ccctgctctc gctcataccc aatgcctaca
     2701 tccaaaactt cgagcaggtg aaagccggcg gtcgcttcga gtttggcgcc caggccaaag
     2761 gcatttacaa cggcgaccga gaagaatacc ctgcttttga tgtcaaattg gcggtggcag
     2821 acggcagcgt tcaataccca ggtcttccca tgggtatgga gcaaattcag gccgagatga
     2881 acgtcaacag tcccggcggc aacctcgacg cactctctgt caatgtgccg cgctttggtt
     2941 tattggtagg caagcgtccg ttcgatcttg tgttttttct caaaacgccg ctttccgacc
     3001 ccgatctgga tgctaaaatg aaaggcgtca ttcatttggc cgagtgggcc aaggcgttcc
     3061 ccatggaggg tgtcaacggc ggtatcatag atgccgacgc ccgcataaaa acccgcatgt
     3121 cttacataga caacgggcaa tacgaccgcg tggacatgag cggcaaagtg gccattcaaa
     3181 acctgaaata caaatcggaa ggtctgccgc cggtgcatat ccaatcgctg agcaccgtgt
     3241 ttgcgcccca gcgtgtggaa atcaataact ttgaaggcaa attaggcgaa agcgacctca
     3301 aagcgcgcgg cagcatcaaa aacattctgg cctatttctc tccgaaaaaa accatgtacg
     3361 gcgacctggc cttcagttcg caattcttca acatcaacga atggatgccc actcaggaag
     3421 atgccgagcc cgcgccctcg gtggtagatg ctgccccggc cggctccgct acaccgacaa
     3481 caaccgaatc tgaatctgct tccgccatct tcgatcgctt cgattttacg gtggacggca
     3541 gcatccgcga ggtcgtctat tccgattatc gcctgctcaa cacctacgcc aagggcaata
     3601 tcaagcccaa ccgcatggaa attgatgact cgggctttct catcggcgac agcgacatgc
     3661 aggcttcggg cctcgtcctc aatatgtttg actacctgtt caacaacggc accttaggcg
     3721 gcaatctgaa aatcaattcc aaactgatga atctcaatca gttcatggga gaagcacccg
     3781 ccgaaaatgc cgcgcccacg cccagcagca ataccaccac aagctcttcc gacggatcgg
     3841 gctatacgcc ggtgatggtg ccggccaacg taaaaatggc catagacgcc aacatcggac
     3901 ggctgattta taccgattac gaactgagta atctggacgg tcaactggta gtagaagatg
     3961 aaacggtggt ggtgcgcgat gctgtagccg gcttactcgg cggcaaagtg ggcctcacag
     4021 gcggctacga tacccgcgac cctgaaaacc cggccttcaa tatgaagtac gatatgcaga
     4081 acatgagctt ccagcgggtt ttcaaggcgc tcaatacctt caaaatgctc acgcctttgg
     4141 caggctacat tgaaggcaac ttcaataccg cgctcatcat ggaaggcaaa ttggggcaag
     4201 atatgatgcc gcgaatagcc tccctgaacg taaaaggatt tctggaaacc ctcaacggcg
     4261 tcatttccaa cttcaaacct tttcaggaat tggggtccat gcttgacgtg tcttatctca
     4321 aagacaacat caacctgacc aacaccaaaa attggtttga aatacaaaac ggcatcgtgg
     4381 aattgaagga gtttgactac aaatacaaag acatagacat gaaaatcagc ggtacccact
     4441 ccctgatgca agacatgcac tataagataa aggcccgcgt gccccgcaaa ctcttagaaa
     4501 aaaatgtcgc cggagccacc gccagcaaag gttacgacct gctgagaaaa gaagcctcca
     4561 aattcggcgt gaatttgcaa aagagcgagt tcgtcaacat gttgatagac gtgaccggca
     4621 ctactaccaa acccaaagtg gccctgcatt tgctgggcgc cgacggcgaa actccggtgg
     4681 acgacgcggt gaaagaagaa ctcaaagctg aggtggaaaa agccaaagcc gaggtgaagc
     4741 agcaggtaga acaaaaaata gaggagggca aacaaatcgt aaaagagacg gccgacaaag
     4801 cccttgatac tgtaaaaaca gtggccaatc agaaattgga cgagttggaa aaagaggtca
     4861 aagatgctgc caaagagaag ctgaatgaag tagtgggcga taaaattgac agcacggcac
     4921 tgaaaaaagc gcaggaaatt attgacaaaa ataaagaagc cgagcgcatc aaaaaagagt
     4981 tggaaaaatt caatccgttg aaaaagaaga aagaaggagg cggtaggcgg taggcagtgg
     5041 gcggtagccg cgccttgcgc gggccgtggc gtggtaagcg cgagggaaac gcgtcttgca
     5101 gcttgaggct tgaagccagt agccacccgt tccaacccga aaccttgaac cttgaacgaa
     5161 taaaacctga cattaagcag cgaaaacagt tcgggaggta tctctgtaag catcacccgg
     5221 accaaaccac tcacaaagtg accgaagaag aactggcaag aggttgtatc cgggaagaca
     5281 gcgcctgtca gcaggagctt ttccgcaggt atgccggcaa aatgatggcg gtatgtcttc
     5341 ggtatgcgcg tagccatgaa gaagcggaag acctgttgca agactcgttc atcaaggtat
     5401 tcgacggtat tgccgatttt cagttcaaag gttctttaga aggctgggta cggcgcatca
     5461 ccatcaatac ggcgcttcgg cactatcagc gcagcagcct gcggccggaa atgaacggcg
     5521 ccgaatggga actggaaacc gccgaagaac ccgatgtata tggctatctg gaggctgaga
     5581 gtctgatgaa tctcatctcc aaattgccgg acgggtatcg catggtgttc aatttgtatg
     5641 ccatcgaagg ctacaatcat aaagaaatag cagaaatgct gggcattcag gaaagcacct
     5701 ccaggtcgca attggtaaag gcccgcaaga tgctgcagca ttggttggaa gaatttaaaa
     5761 taatagcagt atgaaccgtc gtcaccctgt tgatgatttt ttcaaagaca agctgaaaaa
     5821 ccacagcgtg gagccgtccg caggtatgtg ggaacgcatc gaggaagagc gcaggcgttc
     5881 cgccgtagcg gtcaccccgg cccgattgtc gggttggcat ttgttggcgg tcgctttcat
     5941 cgctgtattg gcgcccctca ccccacgcca aacaaagcaa agcgtgagca atccatcgga
     6001 aatcatcatg gcgcaacagg cagcgcccga ttcggaagct gtagcgccta caggccctct
     6061 cgccgaggcg ccccggttgc aggctgcttt acaaaatgaa ccgacagaaa ccggcatcgc
     6121 ttacgcacag gatgcccgcg acgtcgagca tcatcacagc gcaccggctt tcaccttgca
     6181 tgcacaacgc cccgatatag tggcccatcc tatttctgtt tccacgcacc aattggtgta
     6241 tgatgccgcc gcaaccacag aggctgcttc cgaagagtcg ctctacactc cgatagccga
     6301 gttcgaaccg cttccactcc gccggcattt tgcattggag caggagcgca acggctcctt
     6361 cccggctgcc tcgcagtgtg ccactttcaa aaacatgggc ctgcgatttt atatagaagc
     6421 acacgcctct cccgacctcg ccttccggca gataagtcct gccggcgata gccccgaaca
     6481 ggcggctttc gcccaaaaac gcgaccaaac cgagcgccct ttttacaact atagctttgg
     6541 tgcaaggtta gccgcctcta cccgtttcgg actggtcatg cgtaccgggt tcgattatgc
     6601 tcaaatcaat gagcgccttc gttatgtgac ggaaacagag gagcgtttta ccatcatcaa
     6661 catcatcggg ccggggggca gcgtcattgg agtggatact gtttatgaaa ccatcgtaca
     6721 cgaacataca gctcaaaaca gctaccgaac gctgagcatt cctgtgctgg ccggctacga
     6781 actgcccgtc aagcgctggc tgttctcggc gcagggcggc atacttttaa acttgctttt
     6841 tgctcagcag ggcagtatgt acctgccagg aaccgatatg ccggtgcgtt tttcaacgat
     6901 ggagcaggaa ggccgcccgg cgttcaagtc ccggctgggc ttaggctggt atgcgggcct
     6961 gggcgctgcc tacaagattc gccacaatat gtatctcact gccgaaccgc atgtacgggc
     7021 tttccccggc tctgtgaccc gccatgattt cgataccaaa caaagctata tcagcggtgg
     7081 tttggcattg gggctgaaag tgaagttgta gtagtccagc gcgtatagaa aaagtaaatt
     7141 tagatttgcg ccatcgcttt ctctacattt caaacgtact gcaaaaatga aaattaagta
     7201 ccgcatcact agttgtctgc tgtttttctc ggtcatttgc ctccatgcgc agtctcctca
     7261 agacgccgcc ctcattgacg ctgcctcgca aatgaacttc attgaccggt atcggaatga
     7321 aaccaacggc acctacgtct atggcattcc agggcatgcg ggccgaaacg aaattccgga
     7381 gtcgtttttt aacggcgtac cttacttccg gctgaatgac tactcttaca tacagggtaa
     7441 ttattccggc aaaacgctga accggctgaa caacaaatac ggcgatacct ggctgacgct
     7501 gggcaatatt gatgcggacg ccggcacaat atgtgtaaaa gccggctggt acaatggcct
     7561 cgtcggcgag gttcacttgg aagcacccat gagcagtacc aatattttct ctggagaagg
     7621 cttgctgtac tacaatggcc gggcgatgta cgatatttac atgttctttt ttatgctggg
     7681 cgattggaaa accatgaaag gcgtgtgcgt actgaacccg cgtacatctg acggggtgct
     7741 gcaaacttca acatttgtgt tgaaatccac ctcgccttga cttgtacaga aaatcactgt
     7801 acatttgcac aaaaaatgta tgaatgacat cgaaatggcc agatttgatg ccagaatgac
     7861 gaaagagcaa aaggaatttt ttgaatatgc ctctaagttg ggcgggttca gaagtctgac
     7921 cgaatttgta ctgagcgtcg ttcagtcaaa agcgcatgaa atcgttgaag aggacaacaa
     7981 aatccttgca tcaaagagag atcaggagat tttcttcgac cttgtcacaa aagagacccc
     8041 tcccaatgat gcattgaaat cggcttttca atcttataac agccaatttt gatatgccgg
     8101 agttcttgac gattcccctt gattcgagac acagaaagtc gggcaatcta ttcttgcagg
     8161 caagcaaaaa acaaaccgcc gccctatgac ggaaactcag aattgactat ttttgtaaaa
     8221 gcatgtaagc aaaattttca atctactgat tatgagtgtt ttaaaagata aatatatcaa
     8281 tcccttaact gacttcggtt tcaaaaagct ctttggcaca gagccgaaca agatattgtt
     8341 gatagacttc ctgaatcaaa tcctgccgga gagacatcgg atcacagatt tgagttactc
     8401 ccgaaatgaa catctgggac tgaacgaatt ggatcgcaaa gcgatatttg acctatactg
     8461 catagggaaa tccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnna agcgtctatt atgcctcttt cccgattcag gaacaagcag gtaaaggcga
     8581 atgggattac aggttagagc cggtttactc tgtaggcatc ctagacttca tcttcgatga
     8641 ccacaaatcg gacgaagaac tattgcatac tgtggagttg aaaaatcaac gatgtgaggt
     8701 tttttatgat aaattgaaat tcatttacat cgaattgcca aaatttaaga aacaagagga
     8761 cgaactggag acgcactttg acaaatggct atatgtattc aggcatctgt cgaatcttca
     8821 agacaggcca aaaaaattac aagacagggt atttcaaaaa ctattcgatn nnnnnnnnnn
     8881 nnnnnnnnnc ggcagaaatt gctaaatttt ctccagaaga aagagatgct tatgaagata
     8941 gcttaaaata ctaccgggat ttgaagaacg tggtagatac atcacgagaa gaaggaatga
     9001 tagaaggaat agaaaaaata gccaaaaaga tgaaggaaaa aggaatgtcg aattcagaaa
     9061 tttcagagtt gaccggcctg aatgatgatg aaattaatga tttgtaaaat aagccgac
//
LOCUS       DAORLF010000163         9115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4115_length_9115_cov_1.635982, whole genome shotgun sequence.
ACCESSION   DAORLF010000163 DAORLF010000000
VERSION     DAORLF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4115_length_9115_cov_1.635982"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>278
                     /locus_tag="PK198_13455"
     CDS             <1..>278
                     /locus_tag="PK198_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39791.1"
                     /translation="IVMSNCIEMGLVATNQTANSAVTATFSNVSFTQPNPIAGGQSGI
                     QPTLGQVEFDVFPNPTSGELNLNLEQYIGRSVRIETYSLEGKLLQFSE"
     assembly_gap    279..368
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            789..1343
                     /locus_tag="PK198_13460"
     CDS             789..1343
                     /locus_tag="PK198_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39792.1"
                     /translation="MRNLFTTTWAILLLACCTTAAWSQVDVTATAGTTGPTSYSTVNA
                     AFTAINAGTHQGVITIRITANTTEPATPVPLLRSNSPSNYTSVTIIPSGNVTVNSAAA
                     PTTSRGIIELVGADNVTIDGDDPGTSGTRNLTFQMATTTTTGTQCIRLASNSTTGADG
                     ADNNTVKNCNIIGGRSSATSTTTS"
     gene            1395..>2525
                     /locus_tag="PK198_13465"
     CDS             1395..>2525
                     /locus_tag="PK198_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39793.1"
                     /translation="MNTLIENNIITRCYTGIGAIGASATYLNSGTIIRNNVLGSATSA
                     DNIGSRGILISYSASTAGVGSATIRNNDIRVGDYGATGYSTTIAGIEVGTVNAGLIID
                     RNNIHDINQPSSGGYGAHGIYITGSTSTSGVTMTNNFIRDCKMVVYQTSTTSTFIPTG
                     VFFTAGATNVVLDHNTIVIGTQLGSGANFSSFCVNSSVSGVTFASVRNNILVNTHNST
                     SAYCFYTAATSNISGGTVNNNDYYVTGSASVGYYNSATRSTLSAWQTATGKDAASVSV
                     SPPFVSATDLHIQTSPAPPAVPLFQTGDAGTGVTVDYDNESRATPPCIGADEFVLSIC
                     SSAVAGNITGNSVACVASSNLLSLSGATSGLGVTQQWAYGPVG"
     assembly_gap    2526..2550
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2565..2942)
                     /locus_tag="PK198_13470"
     CDS             complement(2565..2942)
                     /locus_tag="PK198_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39794.1"
                     /translation="MADAVALTPGFTVTTVEDIVEQPDAVTVTLYVPAAAKVAEAMTG
                     FWSLDEKLFGPVQEKVVPMSVKTDRSSGEPTHTGLLLPAVAVGLALMVIAKGAVLLLQ
                     GPQYAQVTVHITSQFPAGRPLVF"
     gene            3016..>3543
                     /locus_tag="PK198_13475"
     CDS             3016..>3543
                     /locus_tag="PK198_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39795.1"
                     /translation="MATYNFAGSSGAYTTLTGTTNPAPTVLTGSTILGDDVGVGNLPI
                     GFTFNYNGSFETVFGISSNGFIQLGNTSATFSGFSSNALASTAKVIAPFWDDNNTTAT
                     TIQYRTAGTAPNRTLTVEWGGIKIGGGGSGTHTLNMQAILYEATGVVQFVYGSSTTGT
                     GTISATIGISGASGNY"
     assembly_gap    3544..3554
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3555..>3923
                     /locus_tag="PK198_13480"
     CDS             <3555..>3923
                     /locus_tag="PK198_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39796.1"
                     /translation="VTPLSPANTSTSSTSTENTGISAFTNIPSGTTYTFTPTGAPSFI
                     YAWSENPVSPTTLAATNIANPMANGITEDKTYTVIVTGNGGCTATSSVTVTAGAALTA
                     TPTATQSNICEGQSSTLAAGA"
     assembly_gap    3924..3933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3934..>5008
                     /locus_tag="PK198_13485"
     CDS             <3934..>5008
                     /locus_tag="PK198_13485"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF39797.1"
                     /translation="GGAPYTYLWDDPAGSTTATISVSPSSTTTYNVIITDDCNATTTG
                     SVTVIVHPVPTATAGSNSPVCVGSALNLTVSTDIGDTFTWSGPNSFSSTLQNPSISNA
                     TSAVAGTYNVTVALGICSATSSTIVTTNPSPVINSTTATPAAICSGGNSTLTVALPGP
                     SSYCTVAFTSSVEAISLVQFADINNSSACATGAGAGGALQDFTSIMGNVVAGSAYTMT
                     LSGTTAGNYTNYFTAFFDWNQDGVFETAVPIGSILNTVCIAQATNTVTVPLTALNGTT
                     RMRIVKNFNASPSNPCGSYSYGQAEDYTLNVTGGANAYTYLWDDPTAATTSSVVVLPV
                     ANTTYNVVVTNANGCSSIGQVAVT"
     assembly_gap    5009..5021
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5316..>5903
                     /locus_tag="PK198_13490"
     CDS             5316..>5903
                     /locus_tag="PK198_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39798.1"
                     /translation="MSTSSCTQPFGYVANNTDLCPADPDKQAPGACGCGIADTDTDGD
                     NTPDCNDGCPNDPNKTAPGACGCGVADVPATWYADTDNDGFGDPNNSVTGFACDQPAG
                     YVANNTDLCPADPNKQVPGACGCGVADVAATYYADADGDGFGAGPAIPGFTCVVPTGT
                     VTNNTDLCPTDPNKQAPGACGCGVADVATTWYADTD"
     assembly_gap    5904..6015
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6016..>7702
                     /locus_tag="PK198_13495"
     CDS             <6016..>7702
                     /locus_tag="PK198_13495"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRF39799.1"
                     /translation="NAINPGATEVVNGIDDNCNGMIDETGLTYYRDMDGDGFGDPNNS
                     MLAGSPPIGYVLDNTDCNDNNALEKPGQTWYKDTDGDNYAETGAVTIIQCTRPTGYKA
                     ASELTANTGDCNDNNAAINPGATEVCNGIDDDCDGSTDEGVLTTYYLDADGDGYYTGA
                     PVTACTSPGAGYVTSVTGGGDCNDGNDDINPGTNELCNGIDDNCNGSTDEGFDADNDG
                     IADCFDNCPNTPNSVIRPIAIDGNIGDFGAPIANGNAGVNYYFSADNTYFYIGVTGVN
                     LGNDNLHIAFSNADGSNNGTNWGVNWSNTPYTYLITIFGPDDICYYPFNDPNGCQQVG
                     NGGGQWQHFAGWSGNPTTEIRIPRAYLGSLNGGSGLVNLTIYSNNNSGTYVWTGYPTS
                     NPQGNAPVTWNSFGQQTYPTYLPQVDADNDGFGAACDCNDNNANINPNATEVCDGIDN
                     DCDSMIDEGLSFTTYYVDNDGDGFGTGNGQSLCSNPGAGYSTVAGDCNDNNADINPNA
                     AEVCDGIDNDCDSMIDEGLSFTTYYVDNDGDGFGTGNGQSLCSNPAAGYSTVAG"
     assembly_gap    7703..7906
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7907..>8219
                     /locus_tag="PK198_13500"
     CDS             <7907..>8219
                     /locus_tag="PK198_13500"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRF39800.1"
                     /translation="GDCNDNNADIKPNATEVCDGIDNDCDGLVDSADPNYVDNTPPTV
                     TCAGTATLNFNGQTGIQIDGPNSPVNLQIVANDNCGIASTMISPSTITASQVGQAVPV
                     TI"
     assembly_gap    8220..8235
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8236..>8649
                     /locus_tag="PK198_13505"
     CDS             <8236..>8649
                     /locus_tag="PK198_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39801.1"
                     /translation="GNTAQCVTTVNVSGLPAGWSSTPAGVGCAGGNNVGYNTSSGTWT
                     VTSTNCFYGPPYTGDSEAFAQRTLCGDGSITALVTGINPLAGGWAGIVMRESNAAGAK
                     KAQLMTNLGSDHRREFRTITNGAVTPQQFSSLSRYW"
     assembly_gap    8650..8714
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8715..>9115
                     /locus_tag="PK198_13510"
     CDS             <8715..>9115
                     /locus_tag="PK198_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39802.1"
                     /translation="FLIGAQTIAMNNCIEMGLIVTNYTANSTVTATFSNVSFTQVTPP
                     SVNTINVQPTLGQVEFDVFPNPTSGALNLNLEQYIGRSVRIETYSLEGKLLQFAELDE
                     VQNTLERLDLSRFQNGMYLVKVKSVGLPDVT"
BASE COUNT         2167 a         2411 c         2076 g         1915 t
ORIGIN      
        1 atcgtgatga gcaactgcat cgaaatggga ttggtagcga ccaatcagac tgccaacagc
       61 gcggtgacgg ctacgttctc caatgtgagc tttacgcagc caaacccgat agcaggaggc
      121 cagtccggca ttcagcctac gctcggacaa gtagagttcg atgtgttccc caacccgaca
      181 agcggcgagt tgaacctcaa cttggagcag tacatcggtc gaagcgtacg catcgaaacg
      241 tacagtctgg aaggcaagct gttgcagttc tctgaattnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnntc ggcctaccgg atgtgacaag gcgaatcgtc aagcaataaa tgatttgatt
      421 cttttttaag gaaacggctg gggtggcaat gcctcagccg tttttgttgg tccgtttttt
      481 acaaaacgcg gctcatcttt tattcctctt caaaaaaaat cttcctccag tgaaaacact
      541 ttgaaaccaa atttttacat ttgtctcgta ctaacgagca gtaaaagaat agcccgcagt
      601 actccccctg cttcttccac cggcataagt tccggtacaa cgattgcacc catatccacc
      661 tgattgaccc attgcattat cagtatggta tcctgctgtt aatgagtgta ttgtatggag
      721 aatatccgcc ggagagccgg tgtgcatata aagcaactat tgattacatt catcaaaatc
      781 ctctatgtat gagaaattta tttacgacaa cttgggccat attgctcctt gcctgctgta
      841 ctactgctgc ctggagccag gtagatgtaa cggccacggc aggcacaacc gggccgacaa
      901 gttattcaac ggtgaatgcg gcatttacag cgattaatgc cggcacacac caaggtgtta
      961 taaccatcag gattaccgcc aacactaccg agccggctac tcctgttcct ttgctgcgca
     1021 gtaactcacc ctctaattac accagtgtta caattatacc ttcgggcaat gtaaccgtaa
     1081 attctgctgc cgctcctaca acgagtcgtg gaattatcga attagtaggt gcagataacg
     1141 taaccattga cggagatgat ccgggtacgt ctggtacgcg aaatctaact ttccaaatgg
     1201 ctactacgac gactacaggt actcagtgca tccgcctcgc ttccaactct accacaggcg
     1261 cagacggtgc cgataacaac actgtaaaaa attgcaacat cattggtggt cgcagtagcg
     1321 ccacctcaac tactacttct tagggtattc tgatgtcgaa tagtacagcc gtaacaactg
     1381 gtgcgtattc gagcatgaat acgctgattg agaataacat catcacccgc tgctatacag
     1441 gcatcggtgc tattggagct agcgccactt atctgaacag cggcaccatt atccgcaaca
     1501 acgtgcttgg cagcgccacg agcgccgaca acataggctc tcgtggtatc ctcattagct
     1561 attccgcaag tactgccggc gtgggttctg ctacaatacg caacaacgac atccgcgttg
     1621 gtgattatgg cgctacgggg tatagtacaa ctattgctgg aattgaggtc ggaacggtga
     1681 acgccggcct gatcattgac cgaaacaaca ttcatgatat taaccaacca agtagtggcg
     1741 gctatggcgc acatggcatc tacatcaccg gatcaacaag cacctccggc gtcactatga
     1801 cgaacaattt catcagagat tgcaaaatgg tggtttacca aacctcgaca acatctacct
     1861 tcatcccaac cggtgtattc tttaccgccg gtgctacgaa cgtagtttta gaccataata
     1921 caatagtaat aggtacacag ctcggttccg gagcaaattt ttcttctttc tgtgtaaatt
     1981 ccagcgtttc tggtgtaacc tttgcctcgg tccgcaacaa cattctggta aatacacaca
     2041 atagcaccag tgcatactgt ttctacactg ctgccacctc aaacatatca ggcggcacgg
     2101 ttaacaacaa tgattactac gttacgggtt cggcaagtgt gggctactat aactccgcta
     2161 ctcgatccac cctctctgcc tggcaaactg ccaccggcaa agatgcagca tcggtaagcg
     2221 tctccccgcc ctttgtcagc gccaccgacc tccacatcca aaccagcccc gcccccccgg
     2281 cagttccact tttccaaaca ggggatgcag gcactggtgt gacagttgac tatgacaatg
     2341 aatcacgagc aacaccacct tgtattggcg ccgatgaatt cgtactttcc atttgttcca
     2401 gcgccgtagc tggtaatatt actggaaata gcgtcgcttg tgtggctagc tctaacttgc
     2461 ttagtctgag cggcgccacc tccggtttgg gcgtgacaca gcagtgggct tatggcccgg
     2521 taggcnnnnn nnnnnnnnnn nnnnnnnnnn ggcactgatg ccactcagaa caccaatggc
     2581 ctgccggcag gaaactggga ggtaatatgt acggttacct gtgcatactg cggcccctgt
     2641 agtaacagca ctgcaccctt tgcgatcacc attaacgcca accctacagc caccgcaggc
     2701 agcaacagcc ccgtatgtgt gggttctccg cttgatctgt ctgttttaac cgacataggc
     2761 actacctttt cctggacggg tccgaacagc ttttcatcaa gcgaccaaaa ccctgtcatt
     2821 gcttccgcta ctttggctgc tgccggtaca tacaatgtaa ctgtcaccgc atcaggttgt
     2881 tccactatgt cttctacggt tgtaaccgtg aatcccggtg tcagcgctac agcatccgcc
     2941 acgcccccta cggtttgtgc aggcggtaat acgcagttga atgtagctgc ggttcagtcg
     3001 tttactacac cggctgtggc aacttataat tttgcaggtt catcaggagc atatactact
     3061 ttaaccggca caacaaaccc agcgccaacg gtgcttaccg gcagcactat tttaggcgat
     3121 gatgtgggcg taggtaatct accgataggg tttactttta actataatgg atcttttgaa
     3181 actgtgtttg gaatatcttc taacggattt attcaattag gaaatacttc agccacattt
     3241 tcaggatttt caagtaatgc tctggcatca acggcaaagg ttatcgcgcc gttctgggat
     3301 gataacaata caacagcaac gactatacaa taccgcaccg caggtacagc acccaataga
     3361 acgctaaccg ttgaatgggg aggtataaag attggtggcg gcggtagcgg cactcataca
     3421 ttaaatatgc aggcaatact gtatgaagcg acgggcgtcg tacaatttgt gtatggttct
     3481 tcaacaacag gtactggaac cataagtgca acaattggta tatccggggc atcgggtaac
     3541 tacnnnnnnn nnnngttaca ccattatctc ctgcaaatac ttccacttca tcaacaagca
     3601 cagaaaacac aggcatatct gcatttacaa atattccaag cggcaccacc tatactttca
     3661 caccaacagg tgcgccatct ttcatctacg cttggtccga gaaccctgta agccctacta
     3721 cattggcagc taccaatatt gccaatccaa tggcaaatgg cattacagaa gataaaactt
     3781 atactgtaat agtaaccggc aatggtggct gtactgctac aagttcagta acagttacgg
     3841 caggtgcggc attaacagct actcctacgg ctacacagag caatatttgc gaaggccaaa
     3901 gctctacttt ggccgcgggt gctnnnnnnn nnnaggcggc gctccatata cttatctgtg
     3961 ggatgaccct gccggatcta caaccgctac catttcggta agcccaagca gcacaactac
     4021 ttataatgta atcattaccg atgactgtaa cgccacaaca acaggttcgg ttacggttat
     4081 tgtacatccg gtacccactg ctaccgcagg cagcaacagc cctgtttgcg ttggttcggc
     4141 actcaacctg acagtgagca ctgatatagg tgatactttt acatggtcag gacctaacag
     4201 ctttagttct acccttcaaa atccaagcat cagcaacgct accagcgcag tagcaggcac
     4261 ttacaatgtg accgtagctc tggggatttg ttcagctact tcctctacga ttgtaacgac
     4321 gaatcccagc cctgtcataa atagtaccac cgctacgccc gctgctatct gttcgggagg
     4381 caatagtact ttgacagtag cgctaccagg tccgtcgtct tactgtaccg ttgctttcac
     4441 gagtagtgtc gaagctatat cgctggtaca gtttgctgac attaataatt ccagcgcttg
     4501 tgcaacaggt gcaggagccg gcggcgcatt acaagacttc acttccatta tgggcaatgt
     4561 agtggctggc agcgcttaca ccatgacctt atccggaact accgccggca actatacaaa
     4621 ttactttacc gccttctttg attggaatca agacggagtc tttgaaactg cggtacccat
     4681 aggcagtatt cttaacactg tctgcatagc gcaagccaca aataccgtaa ccgttccgct
     4741 caccgcgtta aacggtacaa cgcgcatgag aatagtgaaa aatttcaatg cctctccctc
     4801 caatccctgt ggttcttata gttacggtca ggcagaagat tacacattga atgtcacggg
     4861 aggtgctaat gcctacacct acctttggga tgacccgacc gctgccacaa cgtccagtgt
     4921 tgtggtattg cccgtggcta atacgactta taatgttgtc gtaacgaacg ctaatggttg
     4981 ttcttcgatc ggccaggttg cagtaaccnn nnnnnnnnnn ngtctccggc ggcggcacgg
     5041 tatgctccgg cgacccgttg cccaacgtca gcttcgcact gacaggtacg ggaccttgga
     5101 atttgaccta caacaacggc gccaatgatg tgcccgtgac cggcatcaat accagtcctt
     5161 atgtcatcac caatgcgccg gcgggtactt atacagttgt cagcggcagc gatgtcaatt
     5221 gtgcgggtac cggcagcggc agcgtcatag tggccgtgaa cactccgaca acttggtacg
     5281 ccgacaccga caatgacggc ttcggcgacc cgaatatgag tacttcctcc tgtacccagc
     5341 ccttcggcta tgttgccaac aataccgacc tgtgtccggc tgatcccgac aaacaggctc
     5401 caggcgcttg cggctgcggc atagcggata ccgacaccga tggcgataat acccccgact
     5461 gcaacgacgg ctgtcccaac gaccccaaca aaacggcgcc cggcgcttgc ggctgcggcg
     5521 tagcggatgt acctgccact tggtatgcag atacggacaa tgacggtttc ggcgacccga
     5581 acaactctgt tacgggcttc gcctgcgatc aacccgccgg ctatgttgcc aacaataccg
     5641 acctgtgtcc ggctgacccg aacaaacagg tgcccggcgc ttgcggctgc ggcgtagcag
     5701 atgtagctgc cacctactat gctgatgctg atggcgacgg cttcggcgcc ggtcctgcca
     5761 taccgggatt cacctgtgtc gtaccgacag gcaccgtgac caacaatacc gacctgtgtc
     5821 ccaccgatcc taacaagcaa gcccccggcg cttgcggctg cggcgtagca gatgtagcta
     5881 cgacttggta tgcagatacg gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnncaacg ccatcaatcc aggcgctaca gaggtcgtca atggcattga
     6061 tgacaactgc aacggtatga ttgatgaaac cggcctcacc tactaccgcg atatggacgg
     6121 cgacggcttc ggcgacccga acaacagcat gttggcaggc tctccgccta ttggttatgt
     6181 actggacaac acggattgca acgacaacaa cgcattagaa aaaccgggcc agacatggta
     6241 taaagacacg gatggtgata actacgccga aaccggtgct gtgaccatca tccaatgcac
     6301 ccgcccaaca ggttataaag cagcttctga actgacggcc aacaccggag attgcaacga
     6361 caacaacgcg gctatcaacc cgggcgctac cgaagtttgc aacggcatag acgatgactg
     6421 cgacggctct accgacgagg gcgtactgac cacttactac ctcgatgccg acggcgacgg
     6481 ctattacacc ggtgctccgg tgacggcttg cacttcgccc ggcgcgggat atgttacttc
     6541 ggtgaccggc ggcggtgact gcaatgatgg taatgatgat atcaatcctg gcacaaacga
     6601 gctttgcaac ggcattgatg acaactgcaa cggttctacg gacgaaggtt tcgatgccga
     6661 caacgatggc atcgccgact gcttcgacaa ctgcccgaac acgcccaact ccgtcatccg
     6721 ccctattgcc attgatggca acatcggcga tttcggcgct ccgattgcca acggcaacgc
     6781 aggtgtgaac tactacttct ctgccgacaa cacctacttc tacatcggtg taacgggcgt
     6841 gaacctgggt aacgacaacc tacacatcgc cttcagcaac gccgacggca gcaataacgg
     6901 aaccaactgg ggcgtcaact ggagcaacac gccttatact tacctgatta ctatcttcgg
     6961 gccggatgat atttgttact atccgttcaa cgatcctaac ggttgtcagc aggtgggtaa
     7021 tggcggaggc cagtggcagc actttgcagg ctggagcggc aatccaacca cggaaatccg
     7081 cattccgcgc gcttacctcg gctcgctcaa cggcggctcg ggcttggtca atctgaccat
     7141 ttatagcaac aacaatagtg gcacatatgt ctggacaggc tacccgacca gcaatccgca
     7201 gggcaatgct cccgttacct ggaacagctt cggacagcag acatatccta cctacctgcc
     7261 gcaagtggat gcagacaacg acggcttcgg cgcagcctgc gactgcaacg acaacaacgc
     7321 caacatcaat ccgaatgcta ccgaggtctg cgacggcatt gacaatgatt gcgacagcat
     7381 gattgacgag ggtctcagct tcaccactta ttatgtggac aacgacggcg acggcttcgg
     7441 cacaggcaac gggcaatcgc tttgctccaa cccaggggcg ggctactcaa ccgtagccgg
     7501 cgactgcaac gacaacaatg ccgacatcaa tccgaacgct gcggaagttt gcgacggcat
     7561 tgacaatgat tgcgatagca tgattgacga gggcctcagc ttcaccactt attatgtgga
     7621 caatgacggc gacggtttcg gtacgggcaa cgggcaatcg ctctgctcca atccagcggc
     7681 gggctactca accgtagccg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc gactgcaacg
     7921 acaacaatgc cgacatcaaa ccgaacgcta ccgaggtgtg cgacggcatt gacaatgatt
     7981 gcgacggttt ggtggacagc gccgacccga actatgtgga caacacgccg cccaccgtca
     8041 cctgcgccgg cacggccact ttgaacttca atggccagac cggcattcag attgacggcc
     8101 ccaacagccc ggtgaacctg caaatcgttg ccaacgacaa ctgcggcatc gcaagcacca
     8161 tgatttcgcc aagtaccatc actgcctctc aggtgggaca ggccgtaccg gtgaccattn
     8221 nnnnnnnnnn nnnnnggcaa tacagcccaa tgcgtcacca ccgtaaacgt atcgggattg
     8281 ccggcaggct ggtcgtcaac ccccgctggg gtgggatgtg caggcggcaa caacgtaggt
     8341 tacaatacca gttccggcac ttggaccgtg accagcacca actgcttcta tggcccaccc
     8401 tacaccggcg acagtgaagc attcgcacaa cgcacactct gcggcgacgg cagcattaca
     8461 gctttggtga ccggcatcaa cccgcttgcc ggcggctggg caggcatcgt catgcgtgag
     8521 agcaatgcag ccggagcgaa gaaggcacaa ctgatgacga acttaggcag cgatcaccgc
     8581 cgggagttcc gcaccattac caacggcgca gtgaccccgc agcagttctc cagcttgagc
     8641 cgctactggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnngttcct cattggcgca cagaccatcg cgatgaacaa ctgcatcgaa
     8761 atgggcttga tcgtgaccaa ttacacggcc aacagcacag tgacggctac attctccaat
     8821 gtgagcttta cacaggtgac tccgccttcg gtgaacacaa taaatgtgca acccacgctg
     8881 ggtcaggtag aattcgatgt gttccccaac ccgacttcgg gcgcgttgaa cctcaacttg
     8941 gagcagtaca tcggtcgcag cgtacgcatc gaaacgtaca gtctggaagg caaactgttg
     9001 cagttcgctg aattggacga agtgcaaaac acgctggaac gcctcgactt gagcaggttc
     9061 caaaacggta tgtacttagt gaaagtgaag tctgtcggcc tgccggatgt gacaa
//
LOCUS       DAORLF010000164         9105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4121_length_9105_cov_1.962431, whole genome shotgun sequence.
ACCESSION   DAORLF010000164 DAORLF010000000
VERSION     DAORLF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_9105_cov_1.962431"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..361
                     /locus_tag="PK198_13515"
     CDS             209..361
                     /locus_tag="PK198_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39803.1"
                     /translation="MKSKMFRSVLIIAALCMTALSFSSCNRGYGCPTNFSMQSAAGAV
                     ISCIAD"
     gene            372..719
                     /gene="ytxJ"
                     /locus_tag="PK198_13520"
     CDS             372..719
                     /gene="ytxJ"
                     /locus_tag="PK198_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRF39804.1"
                     /translation="MIHWKALESEDQIEAIVARSHEAPCVIFKHSVRCNISYIAKHRL
                     EQKWDFAPDELEAYYLDLIAHRPVSASVAERFGVHHESPQLLLIRNGECTYDASHLDI
                     SVDELHECFHNVF"
     gene            747..1427
                     /gene="mnmD"
                     /locus_tag="PK198_13525"
     CDS             747..1427
                     /gene="mnmD"
                     /locus_tag="PK198_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRF39805.1"
                     /translation="MDHTSLIETQDGSHSVLSHRFGVTYHSKYGAIQESRHVFIEAGL
                     FPKLILQRELAVLELGFGTGLNAFLTLLEAERLERHIFYQTIEAFPLSQEQAAQLNYP
                     EQLELPASRPWLTEMHAAPSDKLLRPTPHFQFRKTIGRFEELDLQNAFDVAYFDAFAP
                     ETQPELWTEEVLGRVYRALRPQGILVTYCAKGAVKRTLKSLGFQIERIPGPPGKREMT
                     RAVKTEAK"
     gene            1492..>2168
                     /locus_tag="PK198_13530"
     CDS             1492..>2168
                     /locus_tag="PK198_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39806.1"
                     /translation="MTTEETILWEKIVAFRLNDDSAAFKFSHRLARENGWTISYSEKV
                     IQEYKKFIFLCCVSPSAVTPSDEVDQVWHLHLTYTKSYWTDLCRNTLAKEIHHTPTKG
                     GFAEGKKFDELYTATLELYKDKFKAKPPKSIWPAKEKRFSDIHFQRVNLKNYWLLRKP
                     DWSINSVAFFVAIVTTLLFIMSQSSGTRGKIIGLLVLFGILLWGIITHKNSGGDSWGG
                     GADSGMAG"
     assembly_gap    2169..2182
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2327..>3959)
                     /locus_tag="PK198_13535"
     CDS             complement(2327..>3959)
                     /locus_tag="PK198_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39807.1"
                     /translation="RFNNTAQSTNKDNGSVIVEGGVGIEKNINVGGNSAVTGTSDVDG
                     IARFNNTTQSTTKDNGAVIVEGGVGIEKNVNIGGDLQATGKLTVGGISQLNGQVTVHA
                     ALGGAQENYDNYPLRVEGASHGVAIKVNATLPDRNINFVTMYDGTGAAMGRIEGFQGL
                     TGVARNIVNDIVNGVSQDSASVYGTSTDQDQAAPNPPSSVTQFFNSNYGFGLLVGTLD
                     FVYSIVQFAINAAAAAGLCIVGDCDDAIWAGISMVVNGVKLGGYIAYNELNLGVAYES
                     GGADYAEWLKKADSGEAFVYGDVVGVKGGLISKSFVDAEKFMVISQSPSVIGAMPAKG
                     YEASYEKIAFMGQVQVKVIGTVHRGDYILPTGNGDGFAIAVAPDKMLARDYARIIGIA
                     WGESDGKELFSYVNTAVGINANDMAQTVENMQIVINQMQLALQQLDPDYKPHFFDTGG
                     NDIVRTKPSYTTSPTLQQLALNRYVPSTYSNLQEALAPMVEKAKHQNFDLTQYPYLED
                     LMKNPGDKELAQKTIDHYTRVLDQLERYAASSGRR"
     assembly_gap    3960..4022
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4023..4721)
                     /locus_tag="PK198_13540"
     CDS             complement(<4023..4721)
                     /locus_tag="PK198_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39808.1"
                     /translation="MIQQSIQLKIALLAGGANGKSVYEESHAVTTGQLGAFNIVIGQG
                     QTLNGDFLKVPWSEYEIWMELSVDEDNRGVYEPLSASRLMAVPYAFHAGTADQVKGGE
                     GVEKTAAFWNDLTVPGPHFIGTLDSRDFVMKTTNLERLRITAAGDVNIANSLNVGVDV
                     NAGRDINAGRDLTAGRNLDVDNNAHIGNDLDVDRNANIDGDLTVHGIARFLNTAQSTN
                     KDNGSVIVEGGVGIE"
     gene            complement(4912..5415)
                     /locus_tag="PK198_13545"
     CDS             complement(4912..5415)
                     /locus_tag="PK198_13545"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39809.1"
                     /translation="MKYLSIAVFLFSGIWLSAQSSVERQVFAAQGASVKGRNVQLEWT
                     LGEPAVSTLTTSGGFITEGFQQPTVRVEAVDLMVAAFPKALLDIRVAPNPSPGQLYVQ
                     FPAEMKEDCGWELWNVNGTMLQNRQPATPADETIDISAYPDGMYLLRFSNRQGELLRM
                     YRIVKGR"
     gene            complement(5714..>6127)
                     /locus_tag="PK198_13550"
     CDS             complement(5714..>6127)
                     /locus_tag="PK198_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39810.1"
                     /translation="QRFAPHIEDKAVFLVTGDTRQEDGNPTSAKHGWYRRWKEGLHKF
                     LHKSHPDQAAVQWLEILTGIGFGIVCVYMFQISFLGLCLAFVFLPLMHRLGWEHRFSR
                     VFVFVPFSLIMLGIVLAGLSMAGMQSVFFGYGFHF"
     assembly_gap    6128..6137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6138..>6836)
                     /locus_tag="PK198_13555"
     CDS             complement(<6138..>6836)
                     /locus_tag="PK198_13555"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF39811.1"
                     /translation="HWKWYQPLRNAFGFVMFLGHGALLRRRCWEEVGGFPDIVSEDLG
                     FAIAIREKGYRGRFVEEVVCYEDFPDTVRAFRIRHIKWTRGTSEFLTQKMGGLLRSKN
                     ITWTEKADILFPTLNLPLTLLYFLFMVNANLVMPGFWAHWTEVTWVVGGHEWAMPVLR
                     LDEGFSAIHHADFFFITMLTFFSPVLCFILDMWRKPLQMLRFLAHSTALYAALSPLSS
                     IGVITYAISGKAVF"
     assembly_gap    6837..6911
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6912..7632)
                     /locus_tag="PK198_13560"
     CDS             complement(<6912..7632)
                     /locus_tag="PK198_13560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF39812.1"
                     /translation="MSHRGSYHPIVSMNPVEQPSSTLFSPTKAMDTFQKPNEVGASSP
                     PGPGLYVFIFGTWALAILWFSPRLFQLLDMAHSWGSWAAMISFIAFIHLAWLYGFYNI
                     GVVAFALLHRFWGRNPAPAPALTTFPSVAILYTTCNDFVEESVLSCVHQDYSNYRVYI
                     LDDSSDAGYRHRIDAFAAGFPELVQVIRRPDRKAFKAGNMNHALSRAAITEPYFAIAD
                     ADEILPTDFLSKLVPIMEADPS"
     gene            complement(7670..8992)
                     /locus_tag="PK198_13565"
     CDS             complement(7670..8992)
                     /locus_tag="PK198_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39813.1"
                     /translation="MKKHSILLLILFGPASTLLWGQSMEEAIRWSHSGQHAKAERVLA
                     TLQAEQPANPEITLARAYNLSWMGKQNEAVTEFQKAIRYGADPATARTGMGYAAAFGR
                     HFSLANQTFRKVLQQQPGHQEAARGLALTRFWQGSYQEAIRLLGQQTEAEPNAYDLHI
                     ALGLAQLHSGTPEEARSSFQRALDIQPASAEAKQLIGQTYVTRPFLETNLWLGFTRLD
                     NSENEAGLRGVQATLKWRPDARLWFRYDNSLALDILQFARAGRAAPLFAAGMSRQWDV
                     RFISELEYGVRSLKNRSLQHLVSGAQVWFLPGNVRLKAGGFVGFGAARPVEWMGYGSI
                     NLPAGRNFRIEPTYFFIQPTPGANEHRAQLGLQYRSHNNAECSVYGLYGQTMTPGGEN
                     DSSVYGWSVVGAYPLGRVVSAQAVVRHEQSDAYRFTTAALGVQLRFHK"
BASE COUNT         2182 a         2378 c         2336 g         2047 t
ORIGIN      
        1 tcaagctgca agcctcaagc tgcaagcctc aagctgcaag cctcaagctg caagcctcaa
       61 gctgcaagca ttcaaggaat gtagcttatg tcttgatact tacggcttga agctcagaga
      121 agaatatcaa aaaattgcat acattagaat aattagcctc aactttgcgt tcacaaacgt
      181 gtttttgaaa aacgaaaatc aaattaccat gaaatccaaa atgttccgca gcgtcctcat
      241 catagctgcc ctgtgtatga ccgccctttc tttctcttcc tgcaatcgcg gctacggctg
      301 ccccaccaac ttctccatgc agagcgctgc gggtgcagtg atctcttgca tagccgacta
      361 agcgagcaac catgattcac tggaaagcgc tcgaatccga agaccagatc gaggcaatcg
      421 tcgcccgttc gcacgaggcg ccctgtgtga ttttcaagca cagtgtgcgc tgcaacatca
      481 gctacattgc caaacaccgc ctagagcaga aatgggattt tgcgcctgac gaactggagg
      541 cctattacct cgatctcatt gcacaccgcc ctgtttctgc ttctgtggcc gagcgcttcg
      601 gcgtacacca cgagtcgccc caattgcttc tgatccgcaa cggcgaatgc acttacgatg
      661 cctcccactt agacatttcg gtggacgagt tgcatgagtg ttttcacaac gtcttctgaa
      721 tccggcagtt gccgaatcct ctcaacatgg accacacctc cttgatcgaa acgcaggatg
      781 gctcccattc ggtgttgtcg catcggtttg gagtgacgta ccattccaaa tacggcgcca
      841 ttcaggagtc ccggcatgtg ttcattgagg cgggtttgtt tcccaagttg atcctgcaaa
      901 gagaattggc cgtgctggaa ttgggcttcg gcacgggcct caatgcgttc ctcacactgc
      961 tggaggccga gcgcttggaa cggcatattt tttatcaaac cattgaggcg ttccccttgt
     1021 cgcaagaaca ggctgcccaa ctgaactacc ccgaacaatt agaactgccc gcgtcgcgcc
     1081 catggctgac cgagatgcac gccgcccctt ccgacaaact tctgcgcccc acgcctcatt
     1141 ttcaatttcg caaaaccatc ggccgcttcg aggaacttga tcttcagaat gccttcgacg
     1201 tggcttattt cgacgctttc gcccccgaaa cgcaacccga actctggacc gaagaggtgc
     1261 tgggccgggt atatagagca ctgcgcccgc aaggtatatt ggtgacgtat tgtgccaaag
     1321 gcgccgtgaa acgcacgctc aaaagtctcg gctttcagat agaacgcatc ccaggcccgc
     1381 cgggcaaacg ggagatgacg cgggcggtga agacggaggc gaaatagata ccccatagca
     1441 gcaaaggata agccatgcgg ttgttgatat ctgttaccat caatatatct tatgaccaca
     1501 gaagaaacaa tactttggga gaaaatagtg gcgttcaggc tcaacgacga cagcgcggca
     1561 tttaaatttt cgcacaggct ggccagagaa aatggctgga cgatctccta ttctgaaaag
     1621 gttattcagg aatacaaaaa attcattttt ctgtgttgcg tatctccatc cgcggtgacg
     1681 ccttccgatg aggtggacca agtgtggcat ttgcatttga cctataccaa atcttactgg
     1741 acagaccttt gccgaaatac cctcgccaaa gaaatacatc acacaccgac aaaaggcgga
     1801 ttcgcagagg ggaagaaatt tgacgagcta tacaccgcaa cgcttgagct atacaaagac
     1861 aagtttaagg ccaagccgcc caagagcatt tggcccgcca aagaaaaaag gttttccgac
     1921 atccattttc aaagagtgaa tttgaaaaac tactggttgt tgcgcaagcc tgattggagc
     1981 atcaacagtg tagccttctt tgttgctatt gttacaacgc tgttgtttat catgtcgcaa
     2041 tcaagcggca caagggggaa aatcatcggg ctgctcgtct tattcggcat cctgttgtgg
     2101 ggcattataa cgcataaaaa cagcggcggc gacagttggg gtggaggcgc cgatagcggg
     2161 atggcaggnn nnnnnnnnnn nncggaagtg gctgcggcgg ggattagaaa aacatcgagg
     2221 taaccaatca aataaaaaat ccgcctcaac tttcaacatt gaggcggatg atgcaggcgc
     2281 ccggtttcaa aacgaactcc tccgggcttt attcaaatca atttcgttat cgcctgcccg
     2341 acgaggccgc gtacctttcc aactggtcca atacgcgggt gtaatgatca atggtttttt
     2401 gtgccaattc tttatctccc ggattcttca tcaaatcttc cagatacgga tactgtgtga
     2461 ggtcaaagtt ttggtgcttt gccttttcga ccataggcgc cagagcttcc tgcaaattgc
     2521 tgtaagtaga gggcacataa cgattcaaag ccaactgttg caaagtaggc gaggtcgtgt
     2581 agctaggctt cgtccgaacg atgtcattgc cacccgtatc aaaaaaatgc ggcttgtagt
     2641 cagggtcaag ttgctgaagc gccagttgca tctgattgat aacgatttgc atgttttcta
     2701 ccgtttgtgc catgtcattg gcattgatgc caaccgccgt attgacgtag ctgaacagtt
     2761 ctttgccgtc ggattcgccc catgcaatac cgatgatgcg ggcatagtcg cgcgccaaca
     2821 ttttgtcagg cgcaaccgca atggcaaaac cgtcgccgtt gccggtcggc agaatgtagt
     2881 cgccgcgatg caccgtaccg atcactttca cctgcacctg gcccataaag gcaatttttt
     2941 catagctggc ttcataacct ttggccggca tggcgccgat aacgctcgga ctctgtgaaa
     3001 tgaccatgaa tttctcggca tccacaaagc tcttggaaat aagacctcct ttgacgccca
     3061 caacatcgcc ataaacgaat gcctcgccgg aatcggcctt tttgagccac tcggcataat
     3121 cggcgccccc ggactcatac gcgacaccga ggttcagctc attataggca atatagccgc
     3181 caagttttac tccgttcact accatgctga taccagccca gatggcatcg tcgcagtcgc
     3241 ccacaataca aagccctgcg gctgcggctg cgttgatggc aaactgaaca atggaataaa
     3301 caaagtctaa tgtgcctacc aataacccga aaccgtagtt gctgttaaag aactgcgtca
     3361 ccgaggaagg cggattggga gcggcctgat cttggtctgt gctggtgcca taaacggacg
     3421 ctgagtcctg gctcacgccg ttgacaatgt cgttgacgat gtttctggcc acacccgtaa
     3481 gcccctggaa gccttctatg cgccccatag ccgcacccgt gccgtcgtac atggtcacga
     3541 aattgatgtt gcggtccggc aatgtggcat ttactttgat ggccacccca tgcgaggcgc
     3601 cttctaccct gagcggatag ttgtcgtagt tttcctgagc gccacccaat gcagcatgga
     3661 cggtcacctg gccgttgagt tgagaaatgc cgccaacggt gagcttgccc gtagcttgca
     3721 agtcgccgcc tatgtttaca tttttctcaa tgcccacgcc gccttcgacg atgacagcgc
     3781 cattgtcttt agtggtggat tgagtggtat tgttgaagcg ggcaatgccg tcaacatccg
     3841 aggtgccggt cactgcgctg ttgccgccta cgttgatgtt tttctcaatg cccacgccgc
     3901 cttcaacaat gacggaacca ttgtctttgt tcgtagattg ggccgtattg ttgaaacggn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnctcaatgc ccaccccgcc ttcaacaatg acggagccat tgtctttgtt ggtagattgg
     4081 gccgtattga gaaagcgggc gatgccgtgt accgtgaggt cgccatctat attggcattt
     4141 ctgtccacat cgaggtcatt accgatgtgc gcattattat caacatccag atttcgcccg
     4201 gccgtcaggt cccgtccggc gttgatatcc cgtccggcat tgacgtccac tccgacgttg
     4261 aggctgttgg caatattaac atctcccgcc gccgtgatgc gcaagcgctc cagattggtg
     4321 gttttcatca cgaagtctct gctgtcaagc gtgccgatga agtgcgggcc gggtacggtc
     4381 agatcattcc agaaagcggc cgttttttct acgccttcac ctcctttcac ttggtcggca
     4441 gtgccggcat ggaaagcata gggcaccgcc ataagacggc ttgcgctcaa aggctcataa
     4501 acgccgcggt tgtcttcatc taccgacaac tccatccaga tttcgtactc gctccagggt
     4561 actttcaaaa agtcgccgtt gagcgtttgt ccctggccga tgacaatgtt gaatgccccc
     4621 aattggccgg tagtgaccgc gtggctttct tcatacacag acttgccgtt ggcgccgccg
     4681 gccagcaagg caatcttcaa ttgaatggat tgctggatca tgggaacccc tttggaatcg
     4741 cgggcaaccg cctgataatt gattccggga gggacgccct ggccccaaag tcaaaatcct
     4801 gcaagcagga aaaacagtgt gataaaggat tgcttcttca taagttgtaa gtgttttaag
     4861 tataaaggaa ggtgttcaaa tcaacgagaa tttgcggtgg ccgcaatttt ttcatcgccc
     4921 ttttacaatg cggtacattc tcaacagctc gccttgacgg ttgctgaacc tgagcaaata
     4981 catgccgtcg ggataggcgc tgatgtcaat ggtttcatcg gcaggagtag ccggctgccg
     5041 gttttgcagc atcgtgccat ttacattcca cagctcccag ccgcagtctt ctttcatttc
     5101 tgccggaaac tgcacataga gctgtcccgg gcttggattg ggggccacgc ggatgtccaa
     5161 taaggctttt gggaaagcag cgaccatcag gtccactgct tcaactcgta ctgtgggctg
     5221 ctgaaagccc tcggtaatga accctccgga ggttgtgagc gtagagacgg cgggttctcc
     5281 caatgtccat tccaattgga cattacggcc tttgacggat gctccctgag cagcaaaaac
     5341 ctgtcgttct acggaggatt gggcgctgag ccagatgccc gaaaagagaa acactgcaat
     5401 agataggtac ttcattgcaa atgggttttg gtgaacgaaa taaagagaat tgcatataaa
     5461 acttgatgcc gcaaaattcg gggggtgact tatcaacatc aaaatgctga cgcaataata
     5521 caattatttt ttgtggcggg aaatttttga aaaaaaaatt gcgcatcatt ttgtgcgcgt
     5581 tcatctcaac gccctcacca tccccctcgc cgctttttgt gcggcgccgt gttcgccgag
     5641 gcgcaggcgc aggtcctggt agccttcgcg gagttggcgg gcgtggtggt ctttgagcca
     5701 tgaaacctaa ctgtcagaag tgaaatccat acccaaagaa tacagactgc atacctgcca
     5761 tactcaggcc tgccagcacg atgcccaaca taatgaggct aaaaggcacg aaaacgaata
     5821 cccggctgaa gcggtgctcc caacccaacc ggtgcatgag tggcaggaag acgaatgcga
     5881 ggcaaagtcc cagaaagctg atttggaaca tatacacgca cacgatgccg aaaccaatgc
     5941 cggtaagtat ctcaagccat tgtacggcag cttgatcagg atggcttttg tgaagaaact
     6001 tatgcaaccc ttctttccag cggcgatacc acccgtgctt ggccgatgtg gggttgccgt
     6061 cctcttgccg cgtatccccg gtgaccaaaa agactgcttt atcctcgata tgtggcgcaa
     6121 accgctgnnn nnnnnnnaaa aagactgctt tgccggatat ggcgtaagtg atgacgccga
     6181 tagaagacaa aggcgaaagc gctgcgtaaa gcgccgtgct atgcgccaga aaacggagca
     6241 tttgcagcgg tttgcgccac atatcgagga taaagcacaa gaccggcgag aaaaaagtga
     6301 gcatggtgat gaagaaaaaa tcggcatgat ggatggcgga aaaaccctcg tccaatcgca
     6361 gcaccggcat ggcccattca tggcctccta ccacccaggt cacttccgtc caatgcgccc
     6421 aaaaaccggg cataacgagg ttggcgttga ccataaaaag gaagtagagc aaggtgagcg
     6481 gcaggttgag cgtaggaaac aaaatatccg ctttttctgt ccaggtgatg tttttggaac
     6541 gcagcagccc gcccattttt tgggtcagaa actcactggt gccgcgcgtc cacttgatgt
     6601 gccgaatgcg aaaagcccgc accgtatcgg ggaaatcttc ataacaaacc acctcctcca
     6661 caaagcgtcc gcgatatcct ttttcccgga tggcaatggc aaagccgagg tcctcgctca
     6721 cgatgtcggg aaacccgccg acttcctccc aacaacggcg gcgaagcagc gcaccatgcc
     6781 ccaaaaacat aacaaaaccg aaggcattgc gcagcggctg gtaccatttc cagtgannnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn naggacggat cggcctccat gatgggcacg agtttgctga gaaagtcggt
     6961 cggcaggatt tcatcggcat cggcaatggc aaaatacggc tcagtaatgg ctgcgcggct
     7021 cagggcatga ttcatattac cggccttgaa agctttgcgg tcggggcggc gaatcacctg
     7081 caccaattcc gggaaaccgg cggcaaaagc gtcaatgcga tggcggtacc ccgcatccga
     7141 actatcgtcg aggatgtaaa cccggtagtt ggagtagtct tgatgcacac aagacaggac
     7201 gctttcttcc acaaaatcat tacaggtggt gtacaggatg gccaccgacg ggaaagttgt
     7261 caaagccggc gcgggagcag gatttcggcc ccaaaagcga tgcagcagcg caaaggccac
     7321 cacgccgatg ttgtaaaaac cgtagagcca ggccagatgt ataaatgcga tgaacgaaat
     7381 catggcggcc caactgcccc aggaatgtgc catatcgagc aattgaaaca gccggggcga
     7441 aaaccaaagt atggccagcg cccaagttcc gaaaataaat acatacaggc ccggccccgg
     7501 aggagacgaa gcgcccacct cgttcggttt ttggaaggta tccatcgcct tagtgggcga
     7561 aaaaagtgtt gatgatggct gctccacagg gttcatggag acaataggat ggtagctgcc
     7621 tctgtgtgac atggcatttc ggttttagaa aaacggaggg aaaaagagtt tacttgtgga
     7681 agcgcagttg gacgcccaat gccgccgtag taaagcggta ggcatcactt tgttcgtgtc
     7741 tgacgactgc ctgcgccgat acaacgcgcc ccagcggata ggctccgacg acgctccagc
     7801 catacaccga gctgtcgttt tcgccgccgg gcgtcatggt ttgaccatag aggccgtaca
     7861 cactgcattc ggcattattg tggcttcggt attggaggcc caattgtgcg cggtgttcgt
     7921 ttgcgccggg tgtgggttgg ataaaaaagt aggtaggttc gatgcgaaaa ttccggcctg
     7981 ccggcaaatt gatggatcca tagcccatcc actctacagg gcgggcagct ccgaatccga
     8041 cgaaaccgcc agccttgagg cgaacattgc caggcaggaa ccaaacctgc gcccccgaaa
     8101 cgagatgttg caggctccgg tttttcaagc tgcgaacccc atactccagc tctgaaataa
     8161 agcgcacatc ccattgcctg ctcatgccgg ccgcaaagag cggtgcggcg cgtccggcgc
     8221 gggcaaactg caaaatatcg agggctaaag aattgtcgta tctgaaccat aaacgggcat
     8281 cgggtcgcca tttgagcgtt gcctgtacac cgcgcagccc ggcttcattt tcgctgttgt
     8341 ccaaacgggt aaaaccaagc cataagttgg tttctaaaaa cgggcgcgtc acataagttt
     8401 gtccaattaa ttgtttggcc tctgccgaag ccggctgtat gtccaatgcg cgctgaaagg
     8461 aactgcgggc ttcttcgggc gtgccgctgt gcaattgggc aagacccaaa gcgatgtgca
     8521 aatcataagc gtttggctca gcctctgttt gctgccccag caatctgatg gcctcctgat
     8581 agctgccctg ccaaaaccgg gtcagcgcca agcctctggc ggcctcctga tgaccgggct
     8641 gctgttggag caccttgcga aatgtctgat tggccaacga aaaatggcgg ccaaaggcgg
     8701 cggcatagcc catgccggta cgcgctgtag cagggtcggc cccataccgg atggcctttt
     8761 gaaactcggt gaccgcttcg ttttgctttc ccatccaaga caaattgtag gcgcgcgcca
     8821 aagtaatctc aggattggcg ggttgttcgg cctgcaaagt agcgaggaca cgctcggctt
     8881 tggcatgttg gccgctgtgc gaccatcgga tggcttcttc catgctttgt ccccacagca
     8941 atgtggatgc cggcccaaaa aggataagaa gaaggatgct gtgttttttc atgtcgcttt
     9001 tttttcggta caatgttagg ggcaagtccg aatcttgcca aaaaataggc actcagcggg
     9061 gatatgtgcc gcctcaatcg ggcagggggt ctgctggagg gtttt
//
LOCUS       DAORLF010000165         9103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4122_length_9103_cov_2.008510, whole genome shotgun sequence.
ACCESSION   DAORLF010000165 DAORLF010000000
VERSION     DAORLF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4122_length_9103_cov_2.008510"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK198_13570"
     CDS             <1..190
                     /locus_tag="PK198_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39814.1"
                     /translation="MVYFDWSNNALYRFVNWRKQWNNGTLFVMAYWNPKNSSLPTQSS
                     TQNPYSGAGVQIMYLWNH"
     gene            410..>941
                     /locus_tag="PK198_13575"
     CDS             410..>941
                     /locus_tag="PK198_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF39815.1"
                     /translation="METIIQIHQLSKRFPVGNGEFTALNEVNLTFDKGEFTGLVGPSG
                     SGKTTLLNIIGSLDAPSSGEVTVLGQSIAHLTHAQSAQLRNRHLGFIFQTYNLFPVYT
                     VFENVEFALLLQHLPAAERRKAVMEALEWVGLTDKAQSRPAQLSGGESQRVAIARAMV
                     KKPAIVLADEPTANLDS"
     assembly_gap    942..1018
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1357..1406
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..>1784
                     /locus_tag="PK198_13580"
     CDS             <1407..>1784
                     /locus_tag="PK198_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39816.1"
                     /translation="NGTIDGWNQQARKDTQEWEIGGGQLWHSAYDPLDPLSIQDAHAP
                     LSADMTRLAGQGQLAPILAVQASAFPQGRMQGVLLKGIDPEQKVLKIPTEILQKNPDG
                     ALIGKRMAQALKLKTGDALLVRW"
     assembly_gap    1785..1800
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1801..2475
                     /locus_tag="PK198_13585"
     CDS             <1801..2475
                     /locus_tag="PK198_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF39817.1"
                     /translation="QGAFDALELNIAGIFDCNVPSIDAGQIWLPIERLQTMMNLPGEA
                     TLMVAASGFRPAETSPWVFQSEDVLLADLNTIIQSKKGSGAVLYGLLMIIALLAIFDT
                     QVLSIFRRQREIGTYIALGMTRRQVIGIFTVEGSVYSILAIVLGALYGGPFLWYLAKY
                     GWAMPTGGQDMGISISDRIYPVYGTGLVLGTIILVVIAATVVSFLPARKIARLNPTDA
                     IKGKIQ"
     gene            2484..>3095
                     /locus_tag="PK198_13590"
     CDS             2484..>3095
                     /locus_tag="PK198_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39818.1"
                     /translation="MIKGILRDPNRSVLPVLVISIGVFLSVLFSAWFSGVFSDMIRLN
                     ANFSTGHLKVMTRAYAENAEQMPNDLALLETGSLVADLQRQFPQLDWTQRIRFGGLLD
                     VADAEGNTRAQGMAAGYAVDLFSPGSREAERMNIMPAIVEGRLPAQPGEALISDDFAR
                     RFKVGLGDTVTLFGSTMDGGMSFANFAVAGTVRFGMPVLDRGAI"
     assembly_gap    3096..3115
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3116..>3468
                     /locus_tag="PK198_13595"
     CDS             <3116..>3468
                     /locus_tag="PK198_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39819.1"
                     /translation="SALDMQDAAGEVLGFFKSDQYEEAAALEVKTAFNARFQNDDDEF
                     APLMQRLNDQNGLEEYLVLSKSMSGIMIFVFIFAMSIVLWNTGLLGGLRRYNEFGIRL
                     AMGEEKKRLFRSLII"
     assembly_gap    3469..3504
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3505..3757
                     /locus_tag="PK198_13600"
     CDS             <3505..3757
                     /locus_tag="PK198_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39820.1"
                     /translation="GSVLGTALGLSAAYYLQETGLDFGSAVQGGSMMMPTVFRAHIGA
                     QTFYIGFIPGLFSMVLGNALSGLGIYRRKTAELFKELSV"
     gene            3856..4593
                     /locus_tag="PK198_13605"
     CDS             3856..4593
                     /locus_tag="PK198_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRF39821.1"
                     /translation="MKIIFIFTLFLLLSPALVQQNDANAILRKVEQNMSSDNRVFESE
                     MTIRGKRNSRTITSRSYSVGDKKSFTEYLSPAREAGTKMLKLENQLWIYSPSTDRTIQ
                     ISGHLLRQSVMGSDLSYEDMMDDRKITDVYSAKVAGEETLDGRKTWVLELTAKVADLA
                     YHRRKMWVDAERYVPLKEELYAKSGQLLKRTTLSNVQKIQGRWFPLNVVYKDMLKAGE
                     GTEFKMTSVKFNQDIPASMFTKAALKQ"
     gene            4734..>4932
                     /locus_tag="PK198_13610"
     CDS             4734..>4932
                     /locus_tag="PK198_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRF39822.1"
                     /translation="MQKNTKPRIVFMGTPDFAVPSLRILLDNGFDVAGVVTATDKWGG
                     RGKQQLLQSAVKRFAVERGLPV"
     assembly_gap    4933..4953
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4954..5668
                     /locus_tag="PK198_13615"
     CDS             <4954..5668
                     /locus_tag="PK198_13615"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRF39823.1"
                     /translation="KDPQFLEQLAALDANLQVIVAFRMLPEAVWRMPALGAFNLHGSL
                     LPRYRGAAPINWAVINGDTETGVTTFFLNSEIDTGDLLFQERMTIGPDDTAGDVHDRM
                     MELGAQTVLKTVQAIENQSYTLQKQDEGQASKAPKIFHETCQINFRQSAAEVHNFIRG
                     LSPYPAAWTMLEGEELKILRSSPATGTPPVAPGMFFTDQKSWLRYATNDGWVEVLELQ
                     LQGRRRMSIADFMKGYRFR"
     gene            5725..6672
                     /locus_tag="PK198_13620"
     CDS             5725..6672
                     /locus_tag="PK198_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRF39824.1"
                     /translation="MVKYYIKTDGRLRELEEPRPGCWVNIAPPFSHEELEEVAQSFEV
                     PLDFLTDSLDLDERSRYEREEDVRFILLNSPILNENEDENDAIYVTVPIGIILTLDNI
                     ITISAFDNPIIRLFLDNKVKNFNPSDEKMFVLQIFEHNVYRFLTCLKKLNSKRNLIEQ
                     ELYDSSRNQELKLLLSIEKSLVYFLNSLSSNELLLLKMKRTDYLHIAQDEDLSDLFED
                     IIIDNSQAMEMSKVYSDILGGTMDAYGSIISNNLNLTIRRLTLITILLMVPTLIASFY
                     GMNIRMPFQENNFAFLGILIIALILSAGMAWYFQRKRMF"
     gene            6691..7218
                     /locus_tag="PK198_13625"
     CDS             6691..7218
                     /locus_tag="PK198_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRF39825.1"
                     /translation="MISKTMENALNTQISLEGYASFLYLSMASWCDKEGLQGCAAFMH
                     RQSDEERQHMLRIFHYISEVDGHALAPAIAQPPHSFESVHKLFEQVYAHEQKVTASIN
                     ELLSLAYKDNDHTTLNFLQWYVTEQREEEALMRGILDRIKLIGDGPMSLYYIDKEMEK
                     INKAELAAEAAESAA"
     gene            complement(7304..8809)
                     /locus_tag="PK198_13630"
     CDS             complement(7304..8809)
                     /locus_tag="PK198_13630"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRF39826.1"
                     /translation="MKQQMFFSALIAVFASLLTIGGYHLVSNYQGGGKVRVEHVNGAP
                     SQSAMYTRDENGNFTPLDFTATAEKVMDAVVHIRSTKARSRGDESNPFGGTDDLFEYF
                     FGPRGNRPDSRGGNPQPSIGEGSGVIISQDGYIVTNNHVIADADDIEVTLHDNRSYKA
                     RLIGTDPNTDLAVIQIEEKDLSALAFADSDKVKVGEWVMAVGNPFSLNSTVTAGIVSA
                     KGRNINILREQYAVESFIQTDAAINPGNSGGALVNLDGGLIGINTAIASPTGSYSGYG
                     FAVPANIVSKTVNDLIEYGIVQRGFLGITIRTVDAGLARDKGLKIFKGVYVDSLMEGS
                     SAAKAGVKSGDVIVQIDGQNVTSSSELQEMVARRRPGDQLSLKLNRNGAEKSFSVTLN
                     NRKGNTDLASKAADETSILNTLGIEAEALPSAAAKKMGISGGVRVTSIGAGKIRKNTQ
                     MQEGFIITRVDGKPVSSPEDLLKVLKSKSGGVMLEGRYEDLPGDYYYAFGM"
BASE COUNT         2031 a         2470 c         2377 g         2005 t
ORIGIN      
        1 tatggtgtat ttcgactgga gcaacaacgc actctatcgc ttcgtcaact ggcgaaaaca
       61 atggaacaac ggcacactct tcgtcatggc gtattggaat ccgaaaaaca gcagcctgcc
      121 cacccaaagc agcacgcaaa atccgtactc cggcgcaggt gtgcaaatca tgtacctatg
      181 gaatcattga tgttgaatct ttttgggggc gcaccccgcc actaccagcc accccgaccc
      241 tccaaaggag ggagccatcg ggcaggcggg gccgggctgt ccgctacatg gcggcggctc
      301 gccggagacc ttgcatgcaa cggctctaca aatgccgcca tttcgcttcc atcccttgcg
      361 caactcacac actcacatac tcaccactca catactcacc cactgaaaaa tggaaaccat
      421 catccaaatc catcaactca gcaagcgctt ccctgtcggc aacggcgaat tcaccgcgct
      481 caatgaagtc aatcttacat tcgacaaagg cgaattcacc ggcctggtag gtcccagtgg
      541 atcgggcaaa accaccctgc tcaacatcat cggctcattg gacgcgccca gcagcggcga
      601 agtgaccgta ctggggcagt ccatcgcgca tctcacgcac gctcaatcgg cacaattgcg
      661 caatcggcac ttgggcttca tttttcaaac ctacaacctc tttccggttt ataccgtgtt
      721 tgaaaacgtg gaatttgccc tcctgctcca acacctgccc gctgccgagc gccgcaaagc
      781 cgtgatggaa gcgctggaat gggtgggcct caccgacaaa gcacagtcgc gcccagcaca
      841 gttgtcgggc ggcgagagcc agcgcgtagc cattgcccgc gccatggtca aaaagcccgc
      901 cattgtattg gccgacgaac ctaccgccaa tctcgattcc annnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1021 aggtgatgag ctatctccgg cgcatcatct cgctggaaga cggccgcgtg gtgaaagatg
     1081 aacgaattgc cgcacactca ttcataaaaa gtaacagtta agccacaagc tgttcgctgt
     1141 tagcttttag ctcgtttttt gttaagaaat ttgattttcc acttggggaa attttggatg
     1201 tgcttttgaa aatgaacttt tcaatttttg gaagaccaag ctaacggccg gcagctaacg
     1261 gctaatggct tacgcaggga ccgaataaat ctatttaatc atgattgcca tacaattagc
     1321 actcaaaaac atactcggcg cggggtggcg ctcgctnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnacaa cggcaccatt gacggctgga accaacaggc
     1441 ccgcaaagac acgcaggagt gggaaatcgg cggcggacag ctctggcatt ccgcttacga
     1501 cccattggac ccgctctcta ttcaggatgc ccacgctcct ctttcggcag acatgacccg
     1561 gctggccgga cagggccaat tagcgcccat tctggcggtg caggcttctg cgtttccgca
     1621 aggccgtatg cagggcgtgc tgctcaaagg cattgatcct gagcaaaaag tgctgaaaat
     1681 tcctaccgaa atcctccaaa agaatcccga cggcgccctc atcggcaaac gcatggccca
     1741 ggccctcaag ctgaaaacag gcgacgcgct gctcgtgcgc tggcnnnnnn nnnnnnnnnn
     1801 cagggtgcat tcgacgcgct ggaactcaac attgccggca ttttcgactg caatgtgccc
     1861 agcatagacg ccggccagat ttggctgccg atagaacgcc ttcaaaccat gatgaatctg
     1921 cccggcgagg ccactctgat ggtagcggcc tctggcttcc ggcctgccga gacatcgccc
     1981 tgggtgttcc agtctgaaga cgtcctgctg gccgatctta acaccattat tcagtccaaa
     2041 aaaggctccg gagctgtgct gtacggcctg cttatgatca ttgcgttgct ggccattttc
     2101 gatacgcaag tgctctccat tttcaggcgg cagcgcgaaa tcggcaccta catagcattg
     2161 ggtatgacgc ggcggcaggt catcggcatt ttcaccgtcg agggcagtgt ttacagcatt
     2221 ttggccattg tgctgggcgc tttgtacggc gggccgttcc tctggtattt ggccaaatac
     2281 ggctgggcca tgccgacggg aggtcaggac atgggcattt ctatttccga ccgaatttat
     2341 ccggtttacg gcacgggctt ggtattggga accatcattc tggtagtcat agcggccact
     2401 gtggtgagct ttttgcccgc ccggaaaatc gcccgcttga atccaacaga cgcaataaaa
     2461 ggcaaaatac aatgatccga tttttgataa aaggaatcct gcgggacccc aaccgaagcg
     2521 tgttgccggt gctcgtcatc tccatcgggg tgtttctttc agtgctgttc agcgcctggt
     2581 tttcaggggt tttcagcgat atgatccgcc tcaatgcgaa tttttccacc ggccacctca
     2641 aggtgatgac ccgcgcttac gcggaaaatg ccgagcagat gcccaacgac ctggccctgc
     2701 tggaaaccgg cagcctcgtg gccgatttgc aacgacagtt cccgcaattg gactggacgc
     2761 agcgcatccg cttcgggggc ttgctcgacg tagccgatgc ggagggcaac acccgcgctc
     2821 agggcatggc cgccggatac gccgttgatt tgttcagccc cggcagccgc gaggccgagc
     2881 gcatgaatat catgccggcc atcgtcgaag gccgcctgcc tgcacagccc ggcgaggcgc
     2941 tcatcagcga tgattttgcc cggcggttca aagtcggcct cggcgatacg gtcacgctgt
     3001 tcggttccac tatggacggc ggcatgagct ttgccaattt cgccgtggca ggcacggtgc
     3061 gtttcggaat gccggttttg gaccgcggcg ccatcnnnnn nnnnnnnnnn nnnnnaatct
     3121 gccttagaca tgcaggatgc agccggcgag gtgctcggtt ttttcaaaag cgaccaatac
     3181 gaggaggccg ccgccctgga ggtcaaaacc gcgttcaatg cccggttcca aaacgacgac
     3241 gacgaatttg ctccgctcat gcagcgcctc aacgaccaaa acggtttgga ggaatacctc
     3301 gtgctatcga aaagtatgtc cggaattatg attttcgtgt tcatttttgc catgtccatc
     3361 gtgctgtgga acaccggttt gctgggcggg ctgcgacgct acaacgagtt tggtatccgc
     3421 ttagcaatgg gtgaagagaa gaagcgcttg ttccggtcgc tcatcatcnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnggggtc ggtattgggt acggctttgg ggctgtcggc
     3541 tgcgtattat ttgcaggaaa cgggcctcga tttcggctcg gcagtgcagg gcggcagcat
     3601 gatgatgccc accgtattca gggcgcatat tggtgcgcag acattttaca tcgggttcat
     3661 acccggcctg ttttcgatgg tgttgggcaa tgcgctgtcg ggtctcggca tttaccgacg
     3721 caagacggcc gagttgttta aggagctgtc ggtgtgagaa aataagttta tttcaccagc
     3781 ttagtgaagg gttgactctg caaggttaca gcctgtagtc accccttcac tacgcgtaga
     3841 aaaattccat taaccatgaa aatcattttc attttcactc ttttcctcct cttgtctccg
     3901 gcgcttgtgc agcaaaacga tgccaacgcc atactgcgca aagtggagca aaacatgtct
     3961 tccgacaacc gcgtattcga gtcggaaatg accattcgcg gcaagcgcaa cagccgtacc
     4021 atcacctcgc gctcctattc cgtaggcgat aaaaaatcct tcacggaata cctctcgccg
     4081 gcccgcgagg caggcaccaa aatgctcaag ctcgaaaatc agctatggat ttactcgcct
     4141 tctaccgacc gcaccatcca gatttcgggg cacctgctgc ggcaatcggt catggggtcc
     4201 gacctctctt acgaagacat gatggacgac cgcaagatca ccgacgtcta ttcggccaaa
     4261 gtggcgggcg aagaaaccct cgacgggcgc aaaacctggg tgctggaact gaccgccaaa
     4321 gtggccgacc tggcttacca tcgccgcaaa atgtgggtgg atgccgagcg ttatgtgccg
     4381 ctcaaagagg agttgtatgc caaaagcggg cagttgctca agcgcaccac cctgtcgaat
     4441 gtgcagaaaa tacagggccg ctggtttccg ctcaacgtgg tgtataaaga catgctcaag
     4501 gcaggcgagg gcaccgaatt caagatgacg agcgtgaaat tcaatcagga cattccggca
     4561 agtatgttta ccaaggcggc gctgaagcag tagccgcgct gtggcgcggg atgctaagcg
     4621 cgagcgaaac gcggcttgca gcttgaagcc agtagccggc ggccgttcca aatccgaaac
     4681 cttgaacccg acaccgtcgg ccagtgttcg caggtagctc aaatattcac atcatgcaaa
     4741 aaaatacaaa accacgcatc gtctttatgg gtacccccga ttttgccgtg ccctccctgc
     4801 gcatcctgct cgacaacggc tttgatgtgg cgggcgtagt aacggctacc gacaagtggg
     4861 gcggcagagg caaacaacaa ctactgcaat cggcggtgaa gcggtttgcg gtggagcgcg
     4921 ggctgcccgt ttnnnnnnnn nnnnnnnnnn nnngaaagac ccgcagtttc tggaacaatt
     4981 ggccgcttta gacgccaatc tccaggtcat cgtagctttt cggatgctgc ccgaagcggt
     5041 atggcgtatg cccgcactcg gcgcgttcaa cctgcacggc tcgctgttgc cgcgctatcg
     5101 gggcgccgcg cccatcaact gggctgtcat caatggcgac acggaaaccg gcgtgacgac
     5161 tttttttctc aattccgaaa ttgacaccgg cgacttgctt tttcaggaac gcatgaccat
     5221 cggccccgac gacaccgccg gcgatgtaca cgaccgcatg atggaactcg gcgctcaaac
     5281 cgtcctgaaa acggtgcagg ctattgaaaa ccagagctat acactacaaa aacaagacga
     5341 ggggcaagca tctaaagcgc ccaagatttt tcacgaaacc tgccaaatca acttccggca
     5401 aagcgccgcc gaggtacaca atttcatccg gggtctcagc ccctaccctg ccgcctggac
     5461 gatgctggaa ggcgaggaac tcaaaatcct gcgcagcagc cccgctaccg gcacgccgcc
     5521 cgtagcgccc ggcatgtttt ttaccgacca aaaatcctgg ctccgttatg ccacaaacga
     5581 cggctgggtg gaggtgctgg aactgcaatt gcagggccgt cgtcgaatga gcatcgccga
     5641 ttttatgaaa ggatacaggt ttaggtagat tttttgttgg gcagccacgc gcattgtcgt
     5701 aagtttgagg cacgaaatga acgcatggtc aagtactaca tcaaaacgga cggacggctg
     5761 cgggaactgg aagaaccccg ccccggatgc tgggtcaata tagccccgcc tttcagccac
     5821 gaagagctgg aagaggtcgc gcagtccttt gaagtacccc tcgattttct caccgactct
     5881 ttggacttgg acgaacgctc gcgctacgaa cgcgaggaag acgtgcgttt catcctgctc
     5941 aattcgccca ttctcaacga aaacgaagac gaaaacgacg ccatctatgt caccgtgccc
     6001 atcggcatca ttcttacatt ggacaacatc atcaccattt cggctttcga caaccccatc
     6061 attcgcttgt ttttagacaa taaggtcaaa aatttcaatc cctccgacga aaagatgttt
     6121 gtgttgcaaa tcttcgagca caatgtctat cgcttcctca cctgccttaa aaagctcaac
     6181 tcgaagcgca acctcatcga acaggaactc tacgattcga gtcgaaatca ggaattgaaa
     6241 ttgctgttga gcatcgaaaa aagtcttgtg tattttctca actcgcttag ttccaatgag
     6301 ttgttgctcc tcaaaatgaa gcgcaccgac tatctgcaca ttgcgcagga cgaagacctc
     6361 tccgacctgt ttgaggacat catcattgat aacagccagg ctatggaaat gtccaaagtg
     6421 tattccgaca tcctcggcgg cactatggat gcgtatggct ccattatttc caacaacctg
     6481 aacctgacca tccgccgcct cacgctcatc accatcctcc tcatggtgcc cacgctcatt
     6541 gccagttttt acggcatgaa catacggatg ccttttcagg aaaacaattt tgcctttctc
     6601 ggtattctaa tcattgcctt gatcctcagc gcgggcatgg cctggtattt ccagcgaaag
     6661 cgcatgtttt gatctaaaaa tcagtacgat atgatttcta aaacaatgga aaacgccctc
     6721 aatacgcaaa tctctctgga gggctacgcg tcatttctct acctttcaat ggcttcctgg
     6781 tgcgacaaag aagggcttca gggttgcgcc gcattcatgc accgccaatc ggacgaagaa
     6841 cgccagcaca tgttgcgcat ttttcactac atcagcgagg tggacgggca cgccctcgcg
     6901 ccggccattg cgcagccgcc gcacagcttc gagtcggttc ataaactgtt tgagcaggtg
     6961 tatgcccacg agcaaaaagt gaccgcctcc atcaacgagc ttttgtcgct ggcttacaaa
     7021 gacaacgacc ataccaccct caatttcctt cagtggtacg tcaccgagca gcgcgaagag
     7081 gaggcgctca tgcgtggtat cctcgaccgc atcaaactca tcggcgacgg ccccatgagc
     7141 ctttattaca ttgataaaga gatggagaaa atcaacaaag ccgaactggc cgccgaagct
     7201 gccgaaagcg cggcatagta ccggctgccg acacaaaaag aaagggcttc tcatggaatt
     7261 tgagaagccc ttttttgatg cttacagttt gtttttcaac agattacatc ccgaaagcgt
     7321 agtagtaatc accgggcaag tcttcgtacc ttccttcgag cattacgccg cccgatttgc
     7381 ttttcagtac tttgagcagg tcctccggtg agcttaccgg tttgccgtcc acgcgggtga
     7441 tgatgaatcc ttcctgcatt tgggtgtttt tgcggatttt gcccgcgccg atggaggtga
     7501 cgcgaacgcc gcccgaaatg cccatttttt tagccgcagc cgagggcagc gcctctgcct
     7561 ctatgccgag ggtattgagg atagatgttt cgtcggcagc cttggaagcc aggtcagtat
     7621 tgccttttcg gttgttgagc gtgacggaga acgacttttc tgcgccgttg cggttcaact
     7681 tgagactgag ctgatcgccg gggcggcggc gggccaccat ttcctgcaac tcagacgagg
     7741 aggtgacatt ttggccgtca atctgaacaa tgacatcgcc cgatttgacc cctgcctttg
     7801 cagcggaact gccctccatg aggctgtcca catatacgcc tttgaaaatt ttaagcccct
     7861 tgtcgcgggc caggccagcg tccacggtgc ggatggtgat gccgaggaat ccgcgctgaa
     7921 cgatgccgta ctcgatgagg tcgttcactg ttttgctgac gatattggcc ggcaccgcaa
     7981 agccgtatcc cgaataagaa cctgtggggc tggcaatggc cgtgttgatg ccgatgagtc
     8041 cgccgtcgag attgaccaaa gcgccgccgc tgttgccggg gttgatggct gcatcggttt
     8101 gtataaagct ctctacggcg tattgttcgc gcaagatgtt gatgttgcgg cctttagcac
     8161 tcacgatgcc cgccgttacg gtagagttga ggctaaaagg attgccgacg gccatgaccc
     8221 attcgcccac ttttaccttg tcggaatcgg caaaggcgag ggcggaaaga tctttttctt
     8281 caatctgaat gacggcgagg tcggtattgg gatccgtgcc gatcaagcgg gctttgtagc
     8341 tgcggttgtc gtgcagcgtc acctcaatgt cgtcggcgtc ggcaatgacg tggttgttgg
     8401 taacaatgta gccatcctgg ctgatgatga cgccagaacc ctcgccgatg ctgggttgag
     8461 gatttcctcc gcgggagtcg gggcggttgc cgcgtggccc gaaaaagtac tcaaacaggt
     8521 cgtctgtgcc gccaaaggga ttggactcat cgcctctgga gcgggccttg gtagagcgaa
     8581 tatgcaccac ggcgtccatc actttttcgg cagtggcggt gaagtcgagg ggggtgaaat
     8641 tcccgttttc atcgcgggtg tacatggcgc tttgggaggg cgcgccgttt acatgttcta
     8701 cccgtacctt gccgccgccc tgataattgg aaacgaggtg gtagccgccg atggtgagca
     8761 atgaagcaaa gaccgcaatc aaggcgctga aaaacatttg ttgtttcata gacttttgtc
     8821 gttttgggtg aagaaattat tgatttttgc agtcgccggc aggaatggct gcctgcgttt
     8881 ttcttttttt gaaaatacaa tttgcgttcc agttgccggc ggtgctgtca tattgacagc
     8941 gaacttaacg acatcggagt atctgtatcg tatgccgggc gctgtggttc acagacaaag
     9001 gactgcatgt tctcgacgaa tggttgggac acgaggtaga acctcacgcc ggcgcagtgt
     9061 tgcagcaggg gtggaagcgg taccgacggc agggagcatt ggc
//
LOCUS       DAORLF010000166         9094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4126_length_9094_cov_1.853302, whole genome shotgun sequence.
ACCESSION   DAORLF010000166 DAORLF010000000
VERSION     DAORLF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_9094_cov_1.853302"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>305)
                     /locus_tag="PK198_13635"
     CDS             complement(<1..>305)
                     /locus_tag="PK198_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39827.1"
                     /translation="EHPKAYWRYLLCMAMYHQLLGEFEEVFQYYSKVVDWWDERPANK
                     KEDFYLYIIDVSNLLHAYSVREQYERIPAVLDRLEREEPESLHAKSLLFQKSAVYRL"
     assembly_gap    306..383
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<384..951)
                     /locus_tag="PK198_13640"
     CDS             complement(<384..951)
                     /locus_tag="PK198_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39828.1"
                     /translation="MKNKEDLFQLIKALSKSEKRYFSLDAQKAGRKDSKYMELFQAMN
                     EQDDYDEASLREQFGANLPSDKAYLYEAILRSMRDYHSARSLSARVKERMLDARFLYE
                     RGLPEQSERRLDEAAQLARDLDDRLALLELNIQQRHLTKSLRRKAYQEELKSLIQEKN
                     DHLSALSLELNHMDTADELFVEILQNFQL"
     assembly_gap    1543..1554
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1843..1946
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1947..4141
                     /locus_tag="PK198_13645"
     CDS             <1947..4141
                     /locus_tag="PK198_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF39829.1"
                     /translation="LRSAVPDSLQRDTALTFQLLAKKAWEFNAWDCAAALMEEARRAY
                     ILNRNYNAAAEVQLQVADQLVQRQQTSQAIDELRRALQLPALNPRTIGRLQQALMNAW
                     LLRENESNALIHGRLAAQFFQICGADCHTQAAEVLMTNAFVQIHKGNLPDARKLLEEA
                     QALLRQSNTEERSQTWISLHLLWGDLYAAWNENEEALRRYHRALMAAMPGLSNKDYRV
                     QPDTILPHEQPALAKILAGKADAFRALHRKTKRTEHLARASACYRLSQQAEALLRAPA
                     GEPLHPFFRFDTRTRNQDAIETALELARLQTSIASAEPAFAAAEHEKEQLLKKYLPTR
                     MANSVSLADMQLALPGNDIALIEYVTGAQRLFVFVITKTRYEAISLPLDFPLENWIAQ
                     WRREVEAYQKPGASREALCRSYGAQAFRLYEKLIRPVEKAVSLPQRLVIIPSAELSWI
                     PFEALLTEAAPAGCNYKNYPFLMRRYSISYGISATLQQELMQRPQVRGGYLGLAPVWP
                     ERQESERSLALVQQTTERMRGKALLRDEARREALSGKSLRKSIVHLSAPAQSLLPSAA
                     FVLLSDGQGGTDTLHAAEVQALRMEADLVVLDQHFSSAPGMEAIAWQAGSWLHTGARS
                     VLMPVWTPLGGAHAKVQELFFENLQDEQPKDVALQNAKLQYLQQADSTSAHPAYWAAW
                     ALLGDAGAQTQESGTVMKYLAGLLAVLVLAGAALWLLIRSSHRSSGFG"
     gene            4283..>4984
                     /locus_tag="PK198_13650"
     CDS             4283..>4984
                     /locus_tag="PK198_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39830.1"
                     /translation="MNRTLFILLLTATTLAANAQTLKPWKLNLPAGQTYNVTNTLKNN
                     IEQQVMGQQMNVTSNVVAQMAFSVQSAGAEGFRVEQTNTGMKMETAGMGQSTTFDSNN
                     PSDMAGPIGMQLKGIIGATMEAVIAPDGAISILKGIEMPEGFSFGAGMGDSAALAAYF
                     VNPPANPLRPGDTWTENSDTDGMNTQATYTYRKSKKGRAYLDYEMKMAIVRTITNSGM
                     EIEANIQTEGKGSLVL"
     assembly_gap    4985..5103
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5345..>5622
                     /locus_tag="PK198_13655"
     CDS             5345..>5622
                     /locus_tag="PK198_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39831.1"
                     /translation="MPLKNLFFTALFSLAFFTAFSQDLFPDRCLGQWQGTMHIYFAGQ
                     LRDSVPIRFTVTPIDSISWTWRTEYLSKKLPMTKDYVLRIKDKQRQMYV"
     assembly_gap    5623..5647
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5648..5854
                     /locus_tag="PK198_13660"
     CDS             <5648..5854
                     /locus_tag="PK198_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39832.1"
                     /translation="GIELSDYLFGNKLYSIFETEGILLTASYELRDGELIFEVTSGKR
                     AENSTTGVQSHPVSSVQRAVLKRG"
     gene            6191..6781
                     /locus_tag="PK198_13665"
     CDS             6191..6781
                     /locus_tag="PK198_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF39833.1"
                     /translation="MKSVQNDENAIADIVAGGSRRTKALQLIYQNRALRDMVNAFVRN
                     NKGNETDAQDVFHDAMIILDQNVRNGKFRGEAPLNGYMYSICRFVWMNRMRKQSHTVL
                     PETMPPDEGAEPADAADMRLLDEERRDVLGQLLNTIGERCRKILELWKLSYSMEEIAE
                     QLGFSSADMARKAKYRCHVSLLEQVKNNPKWGDMLR"
     gene            6817..7569
                     /locus_tag="PK198_13670"
     CDS             6817..7569
                     /locus_tag="PK198_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39834.1"
                     /translation="MNLETYIEKIDAYQEGALSETERNAFESELASDAELKAAYDLYL
                     NGNEAIEWQIGESLREQMQVWAQEEKTAGTAPKSGKVVAFRQIRWAAAAALVVLLGLW
                     FVFDRSGSPDNAALFASYYQAPDAAGLRGQEGSDSPLKVGLQLLEEGKGDAARAHFEQ
                     LLATSPNNLEYRYYLGHAAMLQKDWASAEQAFSAAETAGTSSLAHKAAWNKALALLAA
                     NRIDDLKAQLEAIRKDKDHGFYTQAGDLLKKM"
     gene            complement(7580..7774)
                     /gene="xseB"
                     /locus_tag="PK198_13675"
     CDS             complement(7580..7774)
                     /gene="xseB"
                     /locus_tag="PK198_13675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRF39835.1"
                     /translation="MKKAPFSYESAVLELQNIVQQLQEGNVSMDELAERARHAAALIR
                     LCRERLRELETEVDSLFPEG"
     assembly_gap    7879..7889
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7890..9024)
                     /gene="xseA"
                     /locus_tag="PK198_13680"
     CDS             complement(<7890..9024)
                     /gene="xseA"
                     /locus_tag="PK198_13680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRF39836.1"
                     /translation="MADPTTITLFQLQEFIRRVLALNLPDAVWVEAEIAELGQSRGHS
                     YLSLVQKEENSAEPVATAQAVIWQNHLAAIGRKISAALLQELLAPGRQLRIKARIEHH
                     ERFGLKLVVEDIDPAYTIGQLELQRRQTLERLRAEQLTERNAALPTGEVLQRIAVVSS
                     QQGAGYQDFIEHLRGNPYGYRYHCELFPAAVQGQNAEMEMSRSLERIARQAHRFDCTV
                     IIRGGGARLDLGAFDGYDICRAIALHPLPVFTGIGHDVNESLADTCAQLALKTPTAVA
                     DFLIRRHLGFESRLLQLAQDLQTSARQRMAAQRLSVEQIALAVQRHSTQYLLQQNQQL
                     ALLHRDTRREMRNALQSAGKTLDQMNQLSVQLHPQATLERGFTI"
BASE COUNT         2093 a         2309 c         2385 g         1958 t
ORIGIN      
        1 gagccggtaa acagccgatt tttgaaacaa taaactcttg gcgtgcagac tttccggctc
       61 ctcccgctct aagcgatcca gcacggcggg gatgcgctcg tattgttcac gcacagagta
      121 agcgtgcaac aaattggaca catcaataat gtagaggtaa aaatcctctt ttttgttggc
      181 agggcgttcg tcccaccaat ccaccacctt gctgtaatac tgaaacacct cttcaaactc
      241 tcccaataac tggtggtaca tggccatgca caggaggtag cgccagtatg ctttgggatg
      301 ttccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnttagctg gaaattttgc aaaatttcta cgaacagttc
      421 atcggcggta tccatgtgat tcaactctaa agaaagggcc gacaagtggt cgtttttctc
      481 ctgaatgagg ctttttagct cttcctgata ggctttgcgg cgcaggctct tggtgagatg
      541 ccgttgttgg atattgagtt ccaacagagc aagccggtcg tcgaggtcac gggccaattg
      601 tgcggcttca tcaaggcggc gctccgattg ctccggcagg ccgcgctcat agaggaagcg
      661 ggcgtcgagc atgcgttctt ttacgcgggc cgaaagggag cgggcgctgt ggtagtcgcg
      721 catactgcgg aggatggctt cgtagaggta agctttgtcg gagggcaggt tggctccgaa
      781 ctgctcccgg aggcttgctt cgtcgtagtc gtcctgctcg ttcatagcct gaaacaactc
      841 catgtacttg ctgtcctttc taccggcctt ttgggcatcg agactgaaat agcgtttctc
      901 cgatttagag agcgctttga tgagttgaaa aaggtcttct ttgtttttca tttcctacta
      961 ccggaggcgc cattccccct tctatttgtt atcaaattcc taaaaaaata aaaaaaattc
     1021 agttttagaa accccaatta gacagagtag aaagcaaagg taattttttt attaaaaaac
     1081 tgaataacag tattttaatt tcaatgtaac acaatggctt tttgttaaaa atacaacttt
     1141 aggaacccaa actaaagaca ctctcttgtt ttcgcagggc tgtggagcgg tataaatttg
     1201 tacccgtaat cgaaaggatt gcagcacaac attgaaaatc tggttttttt ctgtctatct
     1261 gtccttttat ttcaagactc aatttgccga aatcgggtgg tcttacaagg ctgcccgatt
     1321 ttccatcctg gaaaaagacg gaagccctca tattatgaga aaaaacacta gttcataggg
     1381 caattcggag cgctctgccg ccatcaaaag cggcagagct tttgccctga gcaagtacga
     1441 cacacacgcc ctctgcgggc gaagcatcaa cggaagacag ggtgtttgaa tgggcggggc
     1501 gctcgctgcg caatagcagg gagctgcctc caagcccgaa ccnnnnnnnn nnnnacggtt
     1561 ttcatgttga ttttcattcg gagataagta gatagttgct attattccgg gccgggtggc
     1621 ggtaaaagaa tcgcctcccg gtttactttc ctcccggagc aattgtcgaa aaaagatatt
     1681 atgatttgtt gttagcgcgc acctcaaaaa cccattactg tctgtttcac taattcgagg
     1741 ccggtctcaa gtaccggcct ttttttttgc cctgcacgct acaaatacgc ttactttgca
     1801 aagtccaagt aagtaattat ggctgcacgt tccctcttct ttnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnncgct ccggagtgcc gtacccgatt ccttacagcg
     1981 cgataccgca ttgacttttc aactgttggc caaaaaagcc tgggagttca atgcctggga
     2041 ttgcgccgcc gctctgatgg aggaggctcg gcgggcttat attttgaata gaaattacaa
     2101 cgccgccgcg gaagtccagc ttcaagtggc cgaccagctt gtgcaacgac aacaaacctc
     2161 acaggccata gacgaactcc ggcgcgcgct ccaactaccc gcgctgaatc cgcgaacaat
     2221 agggagacta cagcaggcgc tcatgaacgc atggctgctg cgcgagaacg aaagcaatgc
     2281 gctgattcac gggcgattgg ctgcacagtt tttccaaatt tgcggagctg attgccatac
     2341 acaggccgcc gaggtgctga tgaccaacgc ctttgtacaa attcacaaag gcaacctgcc
     2401 cgatgcccgc aaactgctgg aagaagcgca agcattgctg cgccagtcga atacagaaga
     2461 gcgcagccaa acctggattt cgctgcacct gctgtggggc gacctctatg ccgcatggaa
     2521 cgaaaatgag gaggctctgc gccgctacca ccgcgccctc atggctgcca tgccggggct
     2581 gagcaataaa gactaccgcg tacagccgga taccatattg ccgcacgaac agccggcttt
     2641 ggccaaaata ttagcgggaa aagccgacgc tttcagagcg ttgcaccgga aaaccaaacg
     2701 caccgaacac ctcgcccgcg cctccgcctg ctatcgcctc agccaacaag cggaggccct
     2761 gctccgggcg cccgccggag agccgctgca cccttttttc aggttcgaca cccgcacccg
     2821 caatcaggac gccattgaaa ccgcgctgga actggcccgc ctgcaaactt ccatcgcatc
     2881 cgcagagccg gcttttgctg ccgccgaaca cgaaaaagag caattgctca aaaaatacct
     2941 gcccacgcgc atggccaatt ccgtatcgct tgccgacatg caattggcct taccgggaaa
     3001 tgatattgcc ctcatcgaat atgtgacggg tgcacaacgg ctctttgttt ttgtcatcac
     3061 caaaacccga tacgaggcca tttcgctgcc gctcgacttc cccttggaaa actggatagc
     3121 ccaatggcgc agagaggtgg aggcgtatca aaaacccggc gcctcccgcg aggctttgtg
     3181 caggtcgtac ggcgcgcagg cattccggct ctacgaaaag ctgatccgcc cggtggaaaa
     3241 agccgtttcg ctgccgcagc gattggtcat cattccatcg gctgaattga gttggattcc
     3301 tttcgaggca ctactcaccg aagcggcgcc cgccggctgc aattacaaaa actacccttt
     3361 tttgatgcgt cggtacagca tcagctacgg tatttcggcc accttgcagc aggagttgat
     3421 gcagcggcca caggtacggg gcggttatct gggactggcg ccggtgtggc cggaacggca
     3481 agaatcggag cgctcgctgg cattggtgca acaaactacg gaacgaatgc gcggcaaagc
     3541 cctgctgcgc gatgaggccc gccgggaggc actgtcgggc aagtccctgc gcaaaagcat
     3601 tgtacacctc agcgcaccgg cccaaagcct gctgccgtcc gccgctttcg tacttctgtc
     3661 ggacgggcag ggcggcacag ataccctgca tgccgccgag gtgcaggcat tgagaatgga
     3721 ggccgattta gtcgttctgg accagcattt cagcagtgcg ccgggcatgg aggccatcgc
     3781 ctggcaggca gggagctggc tgcatacggg ggcgcgcagc gtgctgatgc cggtgtggac
     3841 gccgttgggc ggtgcacatg ccaaggtaca ggagttgttt tttgagaatc tccaggacga
     3901 gcagccaaaa gatgtggcgc tgcaaaacgc caaattgcaa tacctccaac aggcagattc
     3961 tacgtccgca caccccgctt actgggcggc ctgggcactg ctgggagatg caggcgcgca
     4021 aacgcaggaa tccggcacgg tgatgaaata tttggcagga ttgttggcgg tgcttgtgct
     4081 ggcgggggcg gcactgtggc tgctgataag gagcagtcac cgtagttcag ggttcggata
     4141 agatcgccca cggtgaaggt ttttacacct gccgtgatcc aggaacaatt gccggccttg
     4201 accaagggtt ctgcggatgc ctcaaatttt aacaattgtt ttaccacctg atgattactt
     4261 ttgcaggcaa aaccttcaaa acatgaaccg tacccttttc atcctgttgc tgactgcaac
     4321 cactcttgcc gccaacgcgc aaaccctcaa gccctggaaa ctcaaccttc ccgccgggca
     4381 aacctacaat gtgaccaaca ccctgaaaaa taacattgag cagcaggtga tgggccaaca
     4441 gatgaatgta accagtaatg tcgtagccca aatggccttc tccgtacaat cggccggcgc
     4501 cgaaggtttc cgggtggaac agaccaacac cggtatgaaa atggagacag ccggaatggg
     4561 acagagcact acgtttgact ccaacaaccc cagtgacatg gccgggccga tcgggatgca
     4621 gctcaaaggg ataatcggcg ccaccatgga ggccgtcatt gcccccgacg gcgccatcag
     4681 catcctgaaa ggcatcgaaa tgccggaagg cttcagtttc ggcgccggca tgggtgattc
     4741 cgccgcgttg gctgcctatt ttgtgaaccc gcccgcgaac cccctccggc ccggagatac
     4801 ctggacggaa aattcagata cagatggtat gaatacgcag gcgacctata cctaccgcaa
     4861 atcgaaaaaa ggacgagcat atttagatta tgagatgaaa atggcaattg tccgcacgat
     4921 taccaacagc ggtatggaaa tagaggccaa tattcaaacc gaaggcaaag gcagcttagt
     4981 cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnngaataac ctgtctccgg ttttgaaaac atgcagcatg gactctgttc cgcggagagg
     5161 tattgctccg ggggagcggc aggtcttaca attttctatt caaagcaggt tctatacccg
     5221 atgaaaaatg gttttcgaaa aaatctaaac catttttcat cgggataact gatagccagt
     5281 ggtttgcaaa aatctaaatt cgcaaaccac tggctatcga gtataaaagc aaacatttca
     5341 gatcatgccg ctcaaaaatc ttttcttcac ggcgctgttc tcactcgcct ttttcactgc
     5401 tttttcacaa gacctttttc ccgaccgttg cctcggacaa tggcagggca ccatgcacat
     5461 ttatttcgca ggccaattgc gcgacagcgt gccgattcgg tttaccgtaa cgcccattga
     5521 ttccatttct tggacctggc gcacggagta cctgtcgaaa aaactgccca tgacaaaaga
     5581 ttatgtattg agaatcaaag acaaacaacg ccagatgtat gtnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnggc attgaactaa gcgattattt gttcggcaac aagctctact ccatttttga
     5701 aaccgagggc atcctgctga cggcaagcta tgaactgcgc gacggggaac tgatcttcga
     5761 agtcacttcg ggcaaaaggg ccgaaaactc aactaccgga gtgcaaagcc atcccgtgag
     5821 cagcgtgcaa cgggcggtgc tgaagcgggg gtgaaggggt ttcgggttca aggttccggg
     5881 ttgcatccgc cgtagccttg gcgaaggtgg gttccgggtt caaggtttca ggtttcgggt
     5941 tgcatctgcc gtagtcttgg cgaaggcggg ttcaaggttt cgggtttcct cgctctgagc
     6001 accgagaacc gcacaccgcc taccgaacac tcacacttag caccccgcgc cacggcgcgg
     6061 ccaccgccta ccgcctactg attccgcgtt ctttttttga aaaattacta actttgagaa
     6121 tcgtaccgca gcattgaaaa aaaatgagtt cggtgggtac atgtttttac ttcacaacac
     6181 atacaggcac atgaaatccg tacagaatga tgaaaacgcc atagccgata tagtggccgg
     6241 aggaagccgg cgaacgaagg cgctgcaatt gatctatcaa aacagggccc tgcgggatat
     6301 ggtgaacgct tttgtaagaa acaataaggg caatgaaacc gatgcgcagg atgtgtttca
     6361 cgatgccatg attatcctgg accagaatgt gcgcaacggg aagttcagag gagaggcgcc
     6421 gctcaacggg tacatgtact ccatctgccg ctttgtgtgg atgaacagaa tgcgcaaaca
     6481 atcgcacacg gtgctgcccg aaaccatgcc acccgacgaa ggcgccgaac cggcagatgc
     6541 agcagatatg aggctcttgg atgaggagcg ccgcgatgtg ctggggcaat tgctcaatac
     6601 catcggcgag cgctgccgca aaattctgga actgtggaag ctctcctatt cgatggaaga
     6661 aattgcagaa caattgggct tttccagtgc ggatatggcc cgaaaagcaa aataccgctg
     6721 ccatgtatcg ctgctggaac aggtgaaaaa caatccaaaa tggggcgata tgttgcgttg
     6781 atcgcatact tcgccaacca ctaaaaaaga cgcattatga atctggaaac ttacatagaa
     6841 aaaatagacg cctaccaaga gggagcgctc tccgagaccg aacgcaacgc cttcgaatcg
     6901 gaactggcat ccgacgccga actgaaagcc gcttacgatc tctatctcaa tggcaatgag
     6961 gccattgagt ggcagattgg cgaaagcctg cgcgagcaaa tgcaggtgtg ggcgcaggaa
     7021 gagaaaaccg ccggtacagc ccccaaaagc ggcaaggtag tcgcctttcg gcagatacgc
     7081 tgggcggctg cggcagcgct ggttgtgctg ttgggcttgt ggttcgtttt tgatcgctcc
     7141 ggcagccccg ataatgcagc gctgtttgcc tcctactatc aggcgcccga cgctgcgggt
     7201 ctgcgcggac aggaaggctc cgacagcccg ctgaaagttg gactgcaatt gctggaagag
     7261 ggaaagggag acgccgctcg cgcacatttt gagcaattgc tggccacctc gcccaataat
     7321 ctggaatatc ggtactatct ggggcatgcg gccatgttgc aaaaagactg ggcttccgcc
     7381 gaacaggctt tctctgctgc cgaaactgcc ggtacttcaa gcctggccca caaagccgcc
     7441 tggaacaagg cgctggcact gctggccgcc aaccgcatag acgatctgaa agctcaattg
     7501 gaggccattc gcaaagataa agatcacggg ttttataccc aggccgggga cttgttgaaa
     7561 aaaatgtaaa ccctactgct cagccttccg gaaacaggct gtccacctcg gtttccagct
     7621 cacggaggcg ctcccggcac agtcggatca acgctgctgc atggcgggcg cgttcggcca
     7681 gttcgtccat acttacattg ccctcctgta attgctgcac gatattttga agttccagca
     7741 cggctgattc gtaagaaaac ggagcttttt tcatggttct gttgatgttg caatgtggcg
     7801 gccgatgctg ccgtcggcaa agatggtttc tatgtggtcg ccgggttgaa tctcggcggc
     7861 agagcgcagg cggcggccnn nnnnnnnnnt gatggtgaaa ccccgttcga gggtagcctg
     7921 cggatgcaat tgcacgctga gctggttcat ttggtccaaa gttttaccgg cagactggag
     7981 cgcattgcgc atttcgcggc gcgtatcgcg gtgcagcagg gccaattgtt ggttttgttg
     8041 tagcaggtat tgtgtactgt ggcgttgtac tgcaagcgcg atttgttcta ctgaaaggcg
     8101 ttgtgctgcc atgcgctgtc gggctgatgt ttgcaggtct tgggcgagtt ggaggagccg
     8161 actttcaaaa ccgaggtgcc tgcgaatgag gaaatcggca acggcggtgg gcgttttgag
     8221 agccaattgg gcgcaagtat ctgccagaga ttcgttcaca tcgtggccga tgccggtaaa
     8281 aacgggcagc gggtgcaggg caatggcgcg gcagatgtcg tagccgtcga atgcgccgag
     8341 atcgagccgg gcgccgcccc cgcgaatgat gacggtacaa tcgaaacgat gcgcctgccg
     8401 ggcaatgcgt tcgaggctgc ggctcatttc catctcagca ttttgtccct gcactgccgc
     8461 cggaaacaat tcgcagtggt atcggtagcc ataaggattg ccgcgcaggt gttctataaa
     8521 atcctgataa cccgcgcctt gctgagaact gacaacggct atccgctgca atacctcccc
     8581 tgtcggcaag gcagcgttgc gctcagtgag ttgttcagcg cgcaggcgtt ccagcgtttg
     8641 gcgacgctgc aattcgagtt gcccgatggt gtacgccggg tcaatatctt cgacgaccaa
     8701 tttcagtccg aagcgttcgt ggtgctcaat gcgggctttg atgcgaagtt gtcggcccgg
     8761 cgccagcagt tcctgaagca acgcagcgct gattttgcga ccaatagcgg cgagatggtt
     8821 ctgccagata acggcttggg cggtggcaac aggttcggcg gagttttcct ctttttgtac
     8881 caaagacaaa taactgtgtc cccttgactg ccccagttcg gcaatttccg cttccaccca
     8941 cacagcatcg ggcaggttca gcgccagcac ccggcggatg aattcctgca attgaaacag
     9001 tgtgatggtt gttggatcgg ccatgccgca aaggtagggg attttgggtt caatgttttg
     9061 ggtttgaggt ttcggggttc atccgccgta ccac
//
LOCUS       DAORLF010000167         9088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4127_length_9088_cov_2.267464, whole genome shotgun sequence.
ACCESSION   DAORLF010000167 DAORLF010000000
VERSION     DAORLF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4127_length_9088_cov_2.267464"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..856
                     /locus_tag="PK198_13685"
     CDS             107..856
                     /locus_tag="PK198_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRF39837.1"
                     /translation="MDILGVDIGGTGLKGAIVDVRSGELRSERVRLETPKPSTPERMT
                     ETFMELVQHLDWTGPVGCGFPAIVRHGVAHSASNIDKGWLGQNIEALFSKASGLHVAA
                     LNDADAAGYAEMDFGKGKGQMGTVLLITIGSGIGTALFVDGREVPNTEFGHLIMHNMI
                     AEHYVSNSARKKLDLSWEKWGKRFNEYLHHLERLLSPDMFILGGGISKDFDSYQKYLK
                     VETPVTPAKLLNNAGIVGAAGYAYRRLGGGA"
     gene            982..1227
                     /locus_tag="PK198_13690"
     CDS             982..1227
                     /locus_tag="PK198_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRF39838.1"
                     /translation="MCLAIPGKIASITNQLDDTFRIGKVSFGGILKEVNLMMVPEAQP
                     GDYVLVHVGVAISVVDEEEAEKTFRYLKEIGELEELE"
     gene            1423..>1818
                     /locus_tag="PK198_13695"
     CDS             1423..>1818
                     /locus_tag="PK198_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF39839.1"
                     /translation="MLKLRLIFSALAIAGSIVSLSAQDIHFSQFYLSPLNLNPALTGV
                     MNCNVRLAANYRNQWASVLRSNAYNTYSVSYDQRIATGRYDYFGVGGTFWGDRAGESR
                     FQTLTGKISGSYSKRMAGTRTSASYLVAGA"
     assembly_gap    1819..1850
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1851..2473
                     /locus_tag="PK198_13700"
     CDS             <1851..2473
                     /locus_tag="PK198_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF39840.1"
                     /translation="NLRWGTQHDGRGAHDPNLPTLENGFNRDNFIFADMAAGLLWFAV
                     FDSDNNLYLGASFHHLNRANLSFTNTEQDLLYSRFTVHGGGEFSMSDKVGLVPGVIVM
                     SQGPSFQVNAGTSLKFILNKTKYDTQAFQVGLWTRVSNKIETGVLNDAAILSTRFDMN
                     NFSLGFSYDINVSPLKAASNGQGGFEFALNYKICGPEKRGLYCPNF"
     gene            complement(2545..>3958)
                     /locus_tag="PK198_13705"
     CDS             complement(2545..>3958)
                     /locus_tag="PK198_13705"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRF39841.1"
                     /translation="WAPLLRCFGFQQLTFFREVFVISGVGVGGGSLVYANTLLTPPDA
                     FFENPVWAKFQDWKTVLAPFYQKAKFMLGATPARHQYEDDRLLAEVAQDMGRGHTYKG
                     VDVGVYYGDPHQPADPYFNGEGPLRKGCIECAGCMVGCRHDAKNTLDKNYLWFAEKYG
                     AQVLPETQATRVTYDDAKGEYTVEARSSTGWLGGGRRSFRARGIVFSAGVLGTMRLLL
                     EQKFGIGTLPRLSDRLGDNLRTNSESLCGVVNADRKLNYGVAISSGFYPDEHTHVELV
                     KFPDKSGAMLRLGSLAVDGHNPTMRLLRWLGAFWVRPLEALRMVFSTNLPRKSVILLV
                     MQTLDNSMRMVWKRGLLGGKMTLDNTAEGASRVPAYIPSGQEVMRRYASKVNGTAMNA
                     VTELAFNMSSTAHIIGGCAMGSDTSEGVVNERFEVFGYPNMYILDGSIVPANLGVNPS
                     LTITALSEYAMSLVPERKGD"
     assembly_gap    3959..3986
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3987..4141)
                     /locus_tag="PK198_13710"
     CDS             complement(<3987..4141)
                     /locus_tag="PK198_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRF39842.1"
                     /translation="MQEKTYDYIVIGSGFGGSVSAMRLAEKGYSVLVLEKGKRYATGD
                     FPRSNWNL"
     assembly_gap    4221..4255
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4320..4937
                     /locus_tag="PK198_13715"
     CDS             4320..4937
                     /locus_tag="PK198_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39843.1"
                     /translation="MEGKLRELTDRIYTDGVERARAEAEQILAQARAEAERISTEAAR
                     AAQRTREQVEEEAARLRDNTQSELARSARQAVSDLQQKISGLVTDRLVAHPVREALSD
                     AGFLQQIIQTAMSNWKPDSQTGPDLQLLLPEKDRERLDAFVAARANELLAKGLHLSYD
                     ERLQDGFRIGPADGSFVVSFSAEDFERFFRMYLRPQVDRQLFGEK"
     gene            4944..5750
                     /locus_tag="PK198_13720"
     CDS             4944..5750
                     /locus_tag="PK198_13720"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRF39844.1"
                     /translation="MTNRQYYYLIAGLPDIVLDQPKMPFSVAAFREELKTHLHPEDYQ
                     LAEWLFLPADNRNLLHFLLKTEATWEENARYSPDDFADGLREPERLPAYMQTFIAAFQ
                     AQEPLRPGMTWENQLTELFYDYALANTQGFLNDWFRFERNLRNILAAMSIRRNRLSVE
                     GQLIGEYEFTAALRSSHARDFGLSGEFPMMERLLQWEDNNWLEREMAIDAKRWTYLDE
                     LNAFHYFSVEVLLAYLIKLMILQRWAPLDKADGDVVFKKIIGDLEHSLPM"
     gene            5944..>6201
                     /locus_tag="PK198_13725"
     CDS             5944..>6201
                     /locus_tag="PK198_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39845.1"
                     /translation="MKNQSTGTVSGIIANLAIIKVEGPVAQNEVCYLRHKDTDLMAEV
                     IKIRSGYAYAQVFESTRGIKVGTPVRFMGHLLEATLGPGILS"
     assembly_gap    6202..6392
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6393..>7218
                     /locus_tag="PK198_13730"
     CDS             <6393..>7218
                     /locus_tag="PK198_13730"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39846.1"
                     /translation="PFMWQGSFTVQSVAPEGEYRVEDEIATLSDASGRQLKVTMTQQW
                     PVKQAVRAYVDKPRPYQLLETGVRTIDTLNPIVEGGTGFIPGPFGSGKTVLQQAISRQ
                     ADADVVIVAACGERANEVVELFKEFPELDDPWHPGRRLMERTVIIANTSNMPVAAREA
                     SVYTAMTIAEYYRSMGLKVLLLADSTSRWAQALREMSNRMEELPGPDAFPMDLSAIIS
                     NFYSRAGMVRLSNNQSGSITFIGTVSPAGGNLKEPVTESTRKAARCFYALSQQRADS"
     assembly_gap    7219..7228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7229..7715
                     /locus_tag="PK198_13735"
     CDS             <7229..7715
                     /locus_tag="PK198_13735"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39847.1"
                     /translation="PAIDPIDSYSKYLEYPELQETLAKAISPDWVDKVNRLKNILLRG
                     KEAAEQINILGDDGVTVDYHLTFWRSEVVDAVILQQDAFDSIDARTPLARQQYMTDWV
                     LRICDTHFQFDSFEQVNPYFKRVVNQMKQMNYSEFESEQFRSFEKELEEMLREGEGSR
                     R"
     gene            7801..>9088
                     /locus_tag="PK198_13740"
     CDS             7801..>9088
                     /locus_tag="PK198_13740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRF39848.1"
                     /translation="MQTQAFQRVYTKIEQLTKATCTLRAQDVGNEELALVHGRLAQVV
                     KIMGDEITLQVFSGTEGIPTNAEVVFFGKPPVLNVSDELAGRFFDAFGRPIDGGPDVQ
                     GEEREIGGPSVNPVRREQPSELITTGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAM
                     VALRAEADKIILGGMGLTNDDYLFYKQVFENAGALHRIVSFVNTTENPPVERLLVPDM
                     ALTAAEYFAIDKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFEAGGSITIIAVTTLSSGDITHAVPDNTGYITEGQLFLRRDTEIGKVIVDPFR
                     SLSRLKQLVIGKKTRADHPQVMNAAIRLYADAANARTKLENGFDLTDYDERTLQFAKE
                     YSDRLLAIDVNIDTTEMLNIAWQLFAKYFSKAEVGIK"
BASE COUNT         2071 a         2558 c         2342 g         1821 t
ORIGIN      
        1 gcaatttgtc tcaactttac gcccgttcag gccatgcgat aacaaacgca cgcttcatga
       61 aaatcggcct tatgcactga ttctaaactt attatcatta ttaaacatgg acatactggg
      121 agttgatatt ggcggcacgg gactaaaagg agccattgta gatgtgcgga gcggagaatt
      181 gcgcagcgag cgcgtacgat tggaaactcc caaaccctcc actccggagc gcatgaccga
      241 aacttttatg gaattagtac agcacctcga ctggaccggg ccggtgggtt gcgggtttcc
      301 ggccattgtg cggcatggcg tggctcattc tgcctccaat attgacaaag gctggctggg
      361 ccaaaacatc gaggcattgt tcagcaaggc ttcggggctc catgtggcag ccctcaacga
      421 cgccgatgct gccggctacg ctgaaatgga tttcggaaag gggaaaggtc aaatgggcac
      481 ggttttactc atcaccattg gatcgggcat aggcaccgcg ctgtttgtgg acggcaggga
      541 agtacccaac acggagttcg ggcatctcat catgcataat atgattgccg agcattatgt
      601 gtcgaactct gcccgcaaaa aattggacct ctcctgggaa aaatggggca agcgcttcaa
      661 cgaatacctg caccatttgg aacgcctgct ttcgccggat atgttcatac tcggaggcgg
      721 catcagcaaa gatttcgaca gttaccaaaa gtatttgaaa gtggaaacac cggtgacgcc
      781 ggccaaactg ctcaataacg cgggcatcgt aggggctgcg gggtatgcgt atcgcaggct
      841 gggaggagga gcatagggca tagagcgaag cgaagtcccg atgaggaacg aaatcgggag
      901 agcatagggc agagggaaat tttgtgagaa aataaactac ttttgccatc ggtattgatt
      961 cgtcaacaac aacattcagt catgtgcctc gccattccgg gtaaaatagc ctccatcacc
     1021 aatcaattgg acgacacctt ccgcataggc aaagtttcgt ttggaggtat cctcaaagaa
     1081 gtgaatctga tgatggtgcc tgaagcccaa cccggcgatt atgtactggt gcatgtgggc
     1141 gtggccatca gtgtggtgga tgaggaagaa gccgaaaaaa cgttccgata tctcaaagaa
     1201 atcggcgaac tggaagagtt ggagtgatcc gctccgcaca aaatatttgc ccatcaggca
     1261 gcgttttggc ctgcacacgg tatctctcta acaattttct accctgatct cattttttaa
     1321 catttaaaaa ttcgccgcta tttgaggcat tgcctattat tgtggcctct ttgtaaccaa
     1381 ccgatttttc taaaggaagc tatcttttca aatccatcat ctatgttgaa acttcgattg
     1441 attttctccg ccctggccat cgccggcagc atcgtgagcc tctctgcgca ggacattcac
     1501 ttctcgcagt tttacctctc gccgctcaac ctcaacccgg ctctgaccgg cgttatgaat
     1561 tgcaatgtcc gattagctgc caactaccgc aaccagtggg cttcggtatt gcgttccaat
     1621 gcctacaata cttactccgt ctcttatgac cagcgcatcg ctaccggtcg ctacgattac
     1681 ttcggcgtag gcggcacttt ctggggcgac cgcgccggcg aatcgcgttt ccaaacgctg
     1741 accggcaaaa tctccggctc ttacagcaag cgcatggcgg gtacccgtac cagcgcgtct
     1801 tacttagtgg ccggggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcaatcttcg
     1861 ttggggtact cagcacgatg gccgcggcgc gcacgatccc aacctgccga cgctggaaaa
     1921 cggtttcaat cgcgacaact tcatcttcgc cgacatggct gccggtttgc tctggttcgc
     1981 cgttttcgat tccgacaaca acctctatct cggcgcttct ttccaccacc tcaaccgcgc
     2041 caatctctct tttaccaaca ccgagcaaga cctgttgtac agccgcttca cggtacacgg
     2101 cggcggcgag ttcagcatga gtgataaggt gggccttgtg ccgggcgtca tcgtgatgag
     2161 ccaggggccg tcctttcagg tgaacgcggg caccagcctc aaattcatcc tcaacaaaac
     2221 caaatacgac acgcaggcat ttcaggtggg cttgtggacg cgcgtttcca ataaaattga
     2281 aaccggcgta ctcaacgatg cagccatcct ctccacccgt ttcgatatga acaacttcag
     2341 cctcggcttc agctacgaca tcaacgtgtc gccgctcaaa gctgccagca acggtcaggg
     2401 cggctttgaa tttgctctga actacaagat ttgcggcccc gaaaaacgcg gcctctactg
     2461 cccgaacttc tgatcgcaca tcagaccaac agacagcgaa aaggggcaca gcagcgtcag
     2521 gttgttgtgc ccttttttgt atgcttaatc ccccttcctt tccggcacca acgacatggc
     2581 gtactcgctc agcgccgtga tggtcaggct ggggttcacg cccagattgg cgggtacgat
     2641 ggagccgtcc aaaatgtaca tattgggata accaaacacc tcaaatcgtt cattcacaac
     2701 gccttccgaa gtgtctgacc ccatagcgca gccgccgatg atgtgcgcgg tggaagacat
     2761 gttgaacgcc agttccgtca ctgcattcat ggccgtgccg ttcactttgg acgcataacg
     2821 ccgcatcacc tcttgcccgg atggaatgta ggcaggcacc cgcgaggccc cttcggcggt
     2881 attgtctaaa gtcattttac cgcccaacaa gccccgtttc cataccattc gcatggaatt
     2941 gtccaaagtt tgcatgacca acaaaatgac cgacttgcgc ggcagattgg tggaaaaaac
     3001 catgcgcaat gcctccagcg gacgcaccca aaacgcgcct aaccagcgca gcaatcgcat
     3061 ggtgggatta tggccgtcca ctgccagaga gccgagccgc agcatggcgc cggatttgtc
     3121 gggaaatttc accaattcca catgcgtatg ctcgtcgggg tagaaaccgg aactgatggc
     3181 cacgccgtag ttgagcttgc ggtcggcatt caccacgccg cagagggatt ccgaattggt
     3241 gcgaaggttg tcgcccagcc tgtcggacaa acggggcagt gtgccgattc cgaatttttg
     3301 ttccaacaag agccgcatgg tacccagcac ccccgcgctg aacacaatgc cccgcgcccg
     3361 gaaactgcgc cgaccaccgc ccaaccagcc cgtactgctg cgggcctcca cggtgtattc
     3421 gcctttggca tcgtcgtaag tgacgcgggt ggcctgcgtt tcgggcagca cctgcgcgcc
     3481 gtacttctca gcaaaccaga ggtagttttt atccaatgtg tttttggcat cgtggcggca
     3541 acccaccatg caacccgcgc actcgataca gcctttgcgc agcggcccct cgccgttgaa
     3601 gtacgggtcg gcgggctgat gcggatcgcc gtaatacacg cccacgtcca cacctttgta
     3661 ggtatggccg cgccccatgt cctgcgctac ttcagccagc aggcggtcgt cttcgtattg
     3721 gtgacgggcg ggggtggcgc ccagcatgaa tttggctttt tgataaaatg gcgccagcac
     3781 ggttttccag tcctgaaact tagcccagac cgggttttca aaaaaagcat ccggcggagt
     3841 cagcagcgta ttggcataaa ccagactgcc gccgcccaca cccacgccgc tgatgacgaa
     3901 cacctcgcgg aaaaaagtga gttgttgaaa cccaaaacag cgcaacagcg gcgcccagnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnaggt tccagttgct gcgcgggaaa tcgccagtgg
     4021 catagcgttt gcctttttcc agcactaaaa cgctgtaccc tttctctgcc aggcgcatgg
     4081 ccgacacgct gcccccgaag ccggagccga tgacgatgta gtcgtaggtt ttttcctgca
     4141 tggtatattg ttttcgcttc ccgcaaagat cgcaaagatt gccaagcatt ggatgtctgt
     4201 tgtcatttga aaagatgaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggtt
     4261 gggcaatctc gtttaattac ctttgctctt gcatgatttc caaatgaaaa acacccatca
     4321 tggaaggtaa actgcgcgaa ctcaccgata gaatatacac cgacggagta gagcgcgccc
     4381 gcgccgaagc cgagcaaata ctggcccagg cccgcgccga agccgagcgc atcagcaccg
     4441 aagctgcccg cgctgcccaa cgcacccgcg aacaagtcga agaagaagcc gcccgcctgc
     4501 gcgacaatac gcagtcggaa ctggcgcgtt cggcccggca ggcggtgtcg gatttgcagc
     4561 aaaaaatttc ggggcttgtc acagatcgct tggtggccca tcccgttcgg gaagcgctct
     4621 ccgatgccgg ttttttgcaa cagatcatcc agacggccat gagcaactgg aagcccgaca
     4681 gccagaccgg ccccgacctg caattgctgc tgcccgaaaa ggaccgcgaa cgtttggacg
     4741 cttttgtggc cgcccgcgcc aatgaactgc tcgccaaagg gcttcacctg agctacgacg
     4801 agcgccttca ggatggtttt cgcatcggcc cggcagacgg gagctttgtg gtttctttca
     4861 gcgccgagga cttcgagcgc tttttccgca tgtatctgag gccgcaggtg gacaggcagt
     4921 tgtttggaga aaaataactt atcatgacca accgccaata ctactacctg atcgccggac
     4981 tgcccgacat cgtgctcgac cagcccaaaa tgcccttttc tgtggcggcg ttcagggaag
     5041 aattgaagac gcacctgcat ccagaagatt accaattagc ggaatggttg tttttaccgg
     5101 ccgacaaccg caatctgctc cattttttgc tcaaaaccga ggcgacctgg gaagaaaatg
     5161 cccgctactc gcccgatgac ttcgccgacg ggctgcgcga accggagcgc ctgccggctt
     5221 atatgcagac tttcatcgct gcgtttcagg cgcaggaacc gctgcggccc ggcatgacct
     5281 gggagaacca actcacggaa ctgttttacg attatgcctt agctaatacg cagggtttcc
     5341 tcaatgattg gttccgattt gagcgcaact tgcgcaatat cctcgcggcc atgagcatcc
     5401 gccggaaccg cctcagcgtg gaagggcagt tgataggcga atacgagttc accgcagcgc
     5461 ttcgcagcag ccatgcccgc gactttggtc tcagcggcga gtttccgatg atggagcgcc
     5521 tcctgcaatg ggaggacaac aactggctgg agcgcgaaat ggccattgat gccaaacgct
     5581 ggacgtattt ggacgaactc aacgctttcc attatttcag cgtggaggtg ttgctcgcct
     5641 atttgatcaa actgatgatt ttgcaacgtt gggcgccttt ggacaaggcc gacggagatg
     5701 ttgtttttaa gaagatcatt ggagatttgg agcatagttt gccgatgtga ttcggtggcc
     5761 gcgccgtggc gcggtaagcg cgaggaacgc gagcgttttt aacgcgaatt ttagcgaagg
     5821 acgcgaattc ccgcgaacca cgcctgtcgt cgtggcgagg agcgagagga accgcggctt
     5881 gcagccagta gccagcagcc gttccaaccc gaaaccaaga acgcgaaaca cgaaatttga
     5941 aacatgaaaa accaatcaac gggaacagta agcggcatca ttgccaacct cgccatcatc
     6001 aaagtggagg ggccggtggc gcaaaatgaa gtgtgctacc tccgccacaa agacaccgac
     6061 ctgatggccg aggtcatcaa aatccgcagc ggatacgcct atgcgcaggt gtttgagagc
     6121 acccgcggca tcaaagtagg cacgccggtg cggtttatgg gccacctgct cgaagccacc
     6181 ctcggtcccg gcatcctgtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgtttat gtggcaaggc agtttcaccg
     6421 tgcaatcggt agcacccgaa ggcgaatacc gcgtagaaga cgaaattgcc accctgagcg
     6481 acgcatcggg tcgccaactg aaagtgacca tgacgcagca atggcctgta aaacaagccg
     6541 ttcgggccta tgtggacaaa ccgaggccct accaactgct cgaaaccggc gtgcgcacca
     6601 ttgacaccct caaccccatt gtggagggcg gcacgggctt cattcccggc cccttcggca
     6661 gcggcaaaac agtgttgcag caggccattt cccgacaagc cgatgccgac gtggtgattg
     6721 tagcggcctg cggagagcgc gccaacgaag tggtggaatt gttcaaagaa tttcccgaac
     6781 tcgacgaccc ctggcatccc ggcagacgcc tcatggagcg caccgtcatc attgccaaca
     6841 cctccaacat gccggtggca gcccgcgagg cttctgttta taccgccatg accatagccg
     6901 aatactaccg cagcatgggc ctgaaagtat tgctgctggc cgattccact tcccgctggg
     6961 cgcaggccct ccgcgaaatg tcgaaccgca tggaggaact gcccggcccc gatgccttcc
     7021 cgatggacct ctcggccatc atttccaatt tctactcgcg tgcaggcatg gtgcgcctga
     7081 gcaataacca aagcggttcc atcaccttca tcggcacggt atcgcccgca ggcggcaacc
     7141 tcaaagagcc ggtgacggaa agcacgcgca aagccgcccg ttgtttttac gctctctcgc
     7201 agcaacgcgc cgacagcann nnnnnnnncc cggccattga cccgatagac agctactcga
     7261 aatatctgga gtaccccgaa ttgcaggaaa cgctggccaa agccatttcg cccgattggg
     7321 tggataaagt gaaccgcctg aaaaacatcc tcttgcgagg caaagaagcc gccgaacaaa
     7381 tcaatatcct cggcgacgac ggcgtcacgg tggactacca cctcaccttc tggcgctcgg
     7441 aagtggtgga tgccgtcatc ctacagcagg acgcttttga ctcgatagac gcccgcacgc
     7501 cgctggcccg gcagcagtac atgaccgact gggtgctgcg gatttgtgac acgcatttcc
     7561 aattcgactc tttcgagcag gtaaatccgt atttcaaaag ggttgtcaac caaatgaagc
     7621 agatgaacta ctcggaattt gaatcggaac agtttcgcag tttcgagaag gaattggagg
     7681 agatgctgag ggagggggaa ggcagtaggc ggtagccgcg ctatggcgcg gggtgcacag
     7741 cttgaggctt gtagcttaag gcttgaggct aaccaaaaaa acaaaaagaa caacgaacac
     7801 atgcaaactc aagcatttca aagggtttat accaaaatag aacagctcac caaagccacc
     7861 tgcaccctgc gcgcacaaga cgtgggcaac gaagaattgg ccttggtgca tggccgtctg
     7921 gcgcaggtag tgaaaatcat gggcgacgag atcaccctgc aagtattctc cggcaccgag
     7981 ggcattccca ccaatgcgga ggtcgtgttt ttcggcaagc cgccggtgct caacgtgagc
     8041 gacgaactcg ccggccgctt cttcgacgcc ttcggacggc ccattgacgg cggcccggat
     8101 gtgcagggcg aggagcgcga aatcggcggt ccttcggtca atcctgtgcg ccgcgagcaa
     8161 cccagcgaac tcatcaccac cggtattgcg ggcatagacc tgaacaacac cttggtgacc
     8221 ggccaaaaga tacccttctt tgccgacccc gaccaaccct acaatcaggt gatggccatg
     8281 gtggccctgc gcgccgaagc cgacaaaatc atcctcggcg gcatgggttt gaccaacgac
     8341 gactaccttt tttacaaaca ggtctttgaa aatgccggcg ccctgcaccg catcgtcagc
     8401 tttgtaaata ccaccgaaaa tccgcccgtg gagcgcctgc tcgtacccga catggccctc
     8461 accgccgccg agtatttcgc cattgacaaa aacgaaaaag tattggtgct gctcaccgac
     8521 atgacgctgt acgccgacgc gctcagcatc gtatcgaacc gcatggacca gattccgtcc
     8581 aaagacagta tgcccggctc gctgtatagc gacttggcca agatttacga aaaagccgtg
     8641 cagttcgagg ccggcggttc catcaccatc atcgcggtga ctacgctttc cagcggagac
     8701 atcacccacg ccgtgcccga caacaccggc tacattactg agggtcagct ctttttgcgc
     8761 cgcgataccg aaatcggcaa ggtcatcgtg gaccccttcc gaagtctgtc gcgcctcaag
     8821 cagttggtca ttggtaagaa gacccgcgcc gatcacccgc aggtgatgaa tgccgccatc
     8881 cgcctctatg ccgacgccgc gaacgcccgc accaagctgg aaaacggctt tgatctgacc
     8941 gactacgacg agcgcacact gcaatttgcc aaagaatact ccgaccgcct cctcgccatt
     9001 gacgtcaaca ttgataccac cgaaatgctg aacattgcct ggcagttgtt tgccaaatac
     9061 ttctccaaag cggaagtggg catcaagc
//
LOCUS       DAORLF010000168         9026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4158_length_9026_cov_1.993312, whole genome shotgun sequence.
ACCESSION   DAORLF010000168 DAORLF010000000
VERSION     DAORLF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_9026_cov_1.993312"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK198_13745"
     CDS             complement(<1..584)
                     /locus_tag="PK198_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRF39849.1"
                     /translation="MSFDKTQIVRALAGVTDPNTGQDIISMSMVRDLEVDGKNINFTL
                     ELPSLNTEHKSELNFACMAAVQQVYPDANVNVHTMSRTPQAQQSTSPTPHIQNIIAVA
                     SGKGGVGKSTVAVNLALGLQKLGARVGLMDADLYGPSIPTMLGLQGERPKIRDVYGQP
                     KMTPLEAYGLYVISIGFIVEPEQAVVLRGPRLSAV"
     gene            663..1544
                     /locus_tag="PK198_13750"
     CDS             663..1544
                     /locus_tag="PK198_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRF39850.1"
                     /translation="MKEKIIGDVEWQHLIIFFASLSATFILAWLFNRLFSKFIKRSSV
                     IIRNDPTNYQFLKHFISAIIYIVGFGVAVYSIPPLRNIATPLLGGAGILAVAIGFASQ
                     NALSNLISGVFIIMFKPFRVNDRLRIRDTLAGIVEDITLRHTVIRDFENRRIIVPNSV
                     MNQEIIINADFSDDNICRWVEVGVGYTSNIDRAKAIMREESEKHPLHIDIRKPEEIAE
                     GKDDVVVRVIQLGEYSILIRAYVWASNQANAFIMGCDLLESIKKRFDAEGVEIPFPYR
                     NIIHHNSQPIESPRELR"
     gene            1604..1972
                     /locus_tag="PK198_13755"
     CDS             1604..1972
                     /locus_tag="PK198_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39851.1"
                     /translation="MMDTDQGYDIAHRRHINWLLQLDYMQDQVRIFQNELSRVIHQHP
                     DFMSVIEHVGEYRAILLKKLHHIDEYRSKIALLERALKNGVWDTPPIKEELLEESLSA
                     FFTEFDTLKITLRRFVSRND"
     gene            2232..2306
                     /locus_tag="PK198_13760"
     tRNA            2232..2306
                     /locus_tag="PK198_13760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2267..2269,aa:Val,seq:gac)
     gene            2435..2508
                     /locus_tag="PK198_13765"
     tRNA            2435..2508
                     /locus_tag="PK198_13765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2469..2471,aa:Pro,seq:ggg)
     gene            complement(2693..>4082)
                     /locus_tag="PK198_13770"
     CDS             complement(2693..>4082)
                     /locus_tag="PK198_13770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF39852.1"
                     /translation="VVTLLVMNDFASLWNGAEASLAWESAAPGGSHILPAMFLNWVWE
                     QTHGNLFLMRLPSVVWLLMAGAGFFYLARPLFGLRTTVTALLVAGTSMGLPNLAKMAT
                     PDSWLFALHLIGFVLLLRHIKQPDRDWRWWFYAVVFASGLIAPWSSLVYFSMLAVFIL
                     GRPGQARQLWRLQAWLPALLAVAVCWFSGTLEWRPDWFFVHQNRPGGYLLLLLGYLPF
                     AGFILSGLRDYAPKLHKGDEMAVLIFAGFMAGMMAQSPAIYFVPALLIARQMDAYFLP
                     VFPYRNFVKAGAVLHLIAAFFGSLILFFGGVYYLQGAGFRVAALIAGAYWSMSFVGVI
                     GLYGMHRRWAIGGPILAGAFALLLFWLSGYPLLESRRKLELDLTNALPADAEALVYVQ
                     CGYHEALPAVAAYVRREFPQTTFVANERKVSGLKRPFYLISRTEIVSCASMFRADSTA
                     AALRLYHCLQTE"
     assembly_gap    4083..4117
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4140..4538)
                     /locus_tag="PK198_13775"
     CDS             complement(4140..4538)
                     /locus_tag="PK198_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF39853.1"
                     /translation="MPHLAHIALVVRDYDEAIEFYTQRLKFDLVEDTPLSDTKRWVLV
                     RPRGAENGCCILLAKAVGEEQQSRIGNQTGGRVFLFLHTDDFERDYRNLLQQDIRIVR
                     PPSQEEYGMVTVFADLYGNLWDLIGKQENL"
     gene            complement(4654..>5664)
                     /locus_tag="PK198_13780"
     CDS             complement(4654..>5664)
                     /locus_tag="PK198_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRF39854.1"
                     /translation="ADCLGRLSANELSDQSDAAFEIADDDVTCQIVVLGSSTAEGTGA
                     QPVADSSWVNRYRKDIFQKNTRYNVVNLARGGYTTYHILPTGTTIPPGVNIAIDTERN
                     ITKALTFNPYAIIVNMPSNDATNNFGPEAQLANFALVKSVANAANAGVWVCTTQPRNF
                     TNQAQILIQTIVRDSILSIYGDNAIDFWTDVAAPNGFISSTYDSGDGIHLNNAGHRLL
                     YERVLAKAIDTIDCTTFTSTNEVLPAQLAGVKVFPNPFQDSFTVEFTTESGGWIELEL
                     MDMLGRRVGKMKEFIGSAGTHQLQWDLPLPAAASQQMLFGLITIQDDKGWARGTVKLT
                     RM"
     assembly_gap    5665..5675
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5676..8803)
                     /locus_tag="PK198_13785"
     CDS             complement(<5676..8803)
                     /locus_tag="PK198_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39855.1"
                     /translation="MKKHVLLLWAMLFFAGYALVAQNDTILIDFGNNLSPAPWNNVTD
                     PVAGSIPDLLNTQGQPSGRSILVYDPFNNINTNGATAPDPALGLPATATGDSFFGNTA
                     TFGGQVQPTGGVELAGLRPGIPHTFTIFASRMSVADNREARYTFTGLTVETAFLDASN
                     NTNRVVTVTLQPDANGVIRIDAAPGPNNTNGTGFYYLGSLVMVYEQAPPPPMPMDTIL
                     VDFGGSNTSLAPWNNVTDPAAGFIADLTNTAGLMTGKSILVYDPFNNINTNGATAPDP
                     ALGLPATATGDSFFGNTATFGGQVQLTGGVELGGLTVGKPHTFTIFASRMGVADNREA
                     RYTFTGQTIETADLDASNNTDRVVTVTLQPDAGGVIRIDAAPGPNNTNGTGFYYLGTL
                     TMSYEHEEPPVVMELDTLLIDFGPVLSPSPWNNLTNPVDGRIDNIINTAGRPSGRGIE
                     VFDAFNNVNTAGTQTPSPAIGFPASASGDSFYGNTGPFAGQVQPTGGVALFNLLPEKP
                     YTITIFASRIGADMREASYLIEGAASETLTLNASSNTDMVVTATIYPAADSTIRITAS
                     PGPNNNTSLGFYHLGAIRVTYEFEEPPVISLIDTLLIDFGNSLTPAPWNNITDPVAGQ
                     IEDILTSAGNASGASIVIYDAFNNINTAGAANPDPAIGFPATATGDSFFGNTATFGGQ
                     VQPTAGLEMSNLLPDKPYTFTIFASRAGVADNREARYTFTGQTIETADLDASNNTDRV
                     ASATLFPNEQGIIRIDVAPGPNNNNSTGFYYLGAMRVVYERNPPPPPAQDTLLLDFGN
                     NFSPLPWNNVADPVAGRIDDLLTSEGFSSGKSVFVYDAFNNINTAGTLSPDPAIGFPA
                     TATGDSFFGNTVLFGGQTQPVGGIEFTNLTPGKPHTFVFFASRTGASDNREARYTLQG
                     LSAETVYLDASNNAANTATATLFPANDGTVRIEVAPGPNNNNSSGFFYLGAMKVIYDE
                     EEAGEPELSVLSPNGGEFWQVGKTPSIIWESKNVGSVILEYSTDNGASWTAIDTVIAV
                     QKQYAWTVPNAPS"
BASE COUNT         2195 a         2347 c         2438 g         2000 t
ORIGIN      
        1 acggccgaaa gcctcggacc gcgcaacacc acagcctgct ctggttcgac tataaaacca
       61 atggaaataa catacaggcc ataagcttcc aaaggggtca ttttgggttg gccatacacg
      121 tcccgaatct tggggcgctc tccctgcaat cccaacatgg tggggatact ggggccataa
      181 aggtcggcat ccatcagccc cacgcgggcc cccaatttct gcaatcctaa agccagattg
      241 acggccacgg tggacttgcc cacgccgcct ttgcccgacg caaccgcaat gatgttttga
      301 atatgcggcg taggactggt actctgctgg gcctgcggtg tgcgggacat tgtatgcaca
      361 ttcacattgg catcgggata gacctgctgt acggctgcca tgcaggcaaa gttcagctcc
      421 gatttatgct ccgtattcaa tgagggcagc tccagcgtga aattgatatt tttgccatcc
      481 acctccaaat cgcgcaccat gctcatactg atgatgtctt gtcctgtatt ggggtcggtt
      541 acgccggcca aagcccttac aatttgtgtt ttatcaaaac tcatgatagt tgtcggtctt
      601 taaaagaatc aaagcaaaca aaccaatatc ctgtgattta tgtttgacgg aaaaaacaaa
      661 atatgaagga aaagatcatt ggcgatgtag agtggcagca tcttatcata ttttttgcat
      721 ctctgtcggc tacgttcatt ttggcatggc tgttcaatcg cctattttcc aaatttatca
      781 aacgatcttc tgttattatc cgaaacgacc ctaccaacta ccaattcctc aaacatttta
      841 tctccgccat aatatacatc gtcggattcg gcgtggccgt gtacagcatt ccgcctctga
      901 gaaacatagc cactccgctt ctgggcggcg ccggcatttt ggccgtagcc attggttttg
      961 cctctcaaaa tgcactcagc aacctcatca gcggcgtgtt catcatcatg ttcaaaccat
     1021 tcagagtcaa tgaccgcctg cgcattcgcg acacgctcgc cgggattgta gaagacatca
     1081 cgctgcggca caccgttatt cgggattttg aaaaccgccg catcatcgtt cccaactctg
     1141 taatgaatca ggaaatcatc atcaatgccg acttcagcga cgacaatatc tgccgctggg
     1201 tagaggtcgg cgtcggttat acttccaata ttgatcgggc caaagccatc atgcgcgagg
     1261 aaagtgaaaa acatccgctt cacatagaca ttcgcaaacc agaggaaatt gccgaaggga
     1321 aagacgatgt ggtggtgcgg gtcattcaat tgggcgagta ctccatactc atcagggcat
     1381 acgtgtgggc ctccaaccag gccaacgctt tcattatggg ctgcgaccta ctggaatcca
     1441 tcaaaaagcg attcgacgcg gagggcgtag aaattccttt cccatacaga aacatcattc
     1501 atcataacag ccaacccatc gaaagcccgc gagagcttcg ttgacaaaag tcatggaata
     1561 attgctaaat tccggctact ttgcctcaaa atcgttggta gttatgatgg acacagatca
     1621 gggctacgac attgcccacc gccgccacat caactggctg cttcagcttg attacatgca
     1681 ggatcaggtc agaatttttc aaaatgaact atctcgcgtg attcaccaac atcctgattt
     1741 catgtcggtt atcgagcatg tgggagagta tagagccatc ctgctcaaaa agcttcatca
     1801 tattgacgaa taccgatcca agattgcact actggaacgg gcactgaaaa acggcgtctg
     1861 ggacacgcct cctataaaag aagaattgct ggaggaatcg cttagcgctt ttttcactga
     1921 gtttgacacc ttgaaaatca ctctgcgtcg ctttgtctca agaaatgact aaaaaataag
     1981 gccgcagaat ttgccgcggc ctcaccaatt cgattagtat gaaaaaactc tatatccaac
     2041 aaacacttct taagtgactt gtattatctg cgcgccctca taaccgacca ttctcaggtt
     2101 taagaagatc aacaccataa gttgcccgac aaaaaatcgt gggaaaaatc ttagccgggt
     2161 attgtacaaa agtgaaacta atcatatctt tgcgcccgct tgcgaaggca ggctgccgtt
     2221 agcattctat cgggtaatta gctcaggtgg tttagagcgc tgctttgaca gagcagaggt
     2281 cacaggttcg agtcctgtat tacccacctc ctctgttttt gaaatatttt cgggatgtcc
     2341 acgcctcggc gtggtggtag agtacacgcc tgggggccgc gctgaaggcg cggaggttca
     2401 aatcctgtca gcctcctgtt ttttgaaata ttttcgggat gtagctcagt ttggtagagt
     2461 acacgtctgg ggggcgtgtg gtcgcaggtt cgaatcctgt catcccgaca catctggggc
     2521 cgcgccaatg gcgcggcctt tctcatttta taccccacac atcatggaac aaaccttcca
     2581 ggtttacatc attcaaagcc aaaagcacgg cagattttac atcggcaggt cacacgatcc
     2641 cgaaaagcga ctccatttcc acaacttagg ctggaacacc tccacgaatg ccctattccg
     2701 tctgcaaaca atgatacaaa cgcaaagctg cggctgtact gtcagccctg aacatggatg
     2761 cgcaagaaac aatctccgta cgcgaaatga ggtaaaacgg tcttttcaac cccgagacct
     2821 ttctctcgtt agccacaaac gtcgtctgcg gaaactcgcg ccgcacataa gccgccaccg
     2881 ccggcagcgc ttcatggtaa ccacattgca cataaacgag cgcttccgca tcagcgggca
     2941 aagcatttgt taaatcaagt tccagctttc ggcgagattc caacaaagga taccccgaca
     3001 accaaaacaa cagcagcgca aaggctccgg ctaaaatcgg gccgcctata gcccaacgtc
     3061 ggtgcattcc gtaaagcccg atcactccca caaaactcat gctccagtat gccccggcta
     3121 tcaatgcagc cacccgaaaa ccagctccct gaagatagta cactcctccg aaaaacaaaa
     3181 tgaggctgcc gaaaaaagcg gcaatcaagt gcagcaccgc cccggcttta acaaagtttc
     3241 ggtagggaaa aacaggcaaa aagtacgcat ccatctgccg ggcaatgagc agcgccggca
     3301 caaaatagat agccggagat tgagccatca tacccgccat aaacccagcg aaaatcagca
     3361 ccgccatttc gtctccttta tgcagcttgg gggcgtaatc tcgcaagccg gaaaggataa
     3421 atccggcgaa gggaaggtag cccaacagca gcagcaaata cccgccgggg cgattctgat
     3481 gcacaaaaaa ccaatcgggc cgccactcca atgtacctga aaaccaacac actgcaacag
     3541 ccaaaagcgc aggaagccag gcttgtaatc gccacaattg ccgggcctgc ccggggcggc
     3601 ccaatataaa aacggccagc atagaaaaat acacgagcga cgaccagggc gctatcaaac
     3661 ccgatgcaaa aaccaccgca taaaaccacc agcgccaatc cctatccggc tgtttgatat
     3721 gtcgcaacaa cagtacaaaa ccgataagat gcagggcgaa gagccaggaa tcgggagtcg
     3781 ccattttggc aaggttgggc aatcccatcg aagtgccggc cacaagcagc gccgtcaccg
     3841 tagtacgcag gccgaaaagc gggcgggcga ggtagaaaaa ccctgccccg gccattaaca
     3901 accacaccac agagggcagt ctcatcagaa aaagattgcc gtgggtttgt tcccaaaccc
     3961 agttcaggaa catcgcaggc agtatatgag acccgcccgg cgcagcgctt tcccaagcca
     4021 aggaggcttc ggcgccgttc cacaaacttg caaagtcatt cattaccaac aaagtaacaa
     4081 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag aaatagcgaa gtgtactggt
     4141 cataggtttt cttgttttcc aatcaaatcc cagagattgc cgtaaaggtc tgcaaaaacc
     4201 gtcaccatac catattcctc ctgactgggc gggcgaacaa tgcggatgtc ttgctgtaga
     4261 agattccgat aatcccgctc aaaatcatcg gtgtgcaaaa acagaaaaac gcgtcctcct
     4321 gtttggttgc cgatgcgact ctgctgttct tcccccaccg ctttagccaa taaaatacaa
     4381 catccgtttt cagcgccgcg aggtctgacg agtacccagc gtttggtatc gctgagcggc
     4441 gtatcttcaa cgaggtcgaa tttcagcctt tgggtataaa actcaatggc ttcgtcgtag
     4501 tctctgacta ccaatgcaat atgtgcaaga tgaggcatat tatatcttga ttgttttgtc
     4561 gttacggatg ctacgtttta tacaaaaaaa ctgccgactt cacattccgc aaagccggca
     4621 gttctgtttt gccaaaaaag aagcagtcgg cgcttacatt cttgtcagtt tcacagtgcc
     4681 gcgagcccag cctttgtcat cctgaatggt gatgaggcca aagagcatct gttgcgaagc
     4741 agctgcgggc aaaggtaagt cccattgcag ttggtgcgta cccgccgagc cgataaattc
     4801 tttcatcttg ccgactctgc ggcccagcat atccatcagt tccagttcga tccagccacc
     4861 cgattcggta gtaaactcaa cggtgaaact gtcctggaaa ggattcggga atactttgac
     4921 acccgccaac tgtgccggaa ggacttcgtt ggtacttgta aaagtcgtgc aatcaatcgt
     4981 atcaatggct ttggccaaaa cccgctcgta cagcagcctg tggccggcgt tgttgaggtg
     5041 aatgccgtcg ccggagtcat aagtgctgct aatgaagccg ttgggcgccg ccacgtccgt
     5101 ccagaagtca atggcattgt cgccgtagat gctcaggatg gaatcccgca cgatcgtctg
     5161 aattagaatc tgcgcctgat tggtgaaatt cctcggctgc gtagtgcaaa cccaaacacc
     5221 cgcattggct gcgttggcta ccgacttgac gagcgcgaag ttggccaatt gcgcctccgg
     5281 cccgaagttg ttggtagcat cgttggaggg catattcacg atgatggcat acggattgaa
     5341 agtcagtgct ttagtaatat ttcgttccgt atctatagca atgttcacgc ccggcgggat
     5401 ggtcgtaccg gtgggcagga tgtgataagt ggtgtaacct ccgcgcgcca ggttgaccac
     5461 gttgtagcgg gtattctttt gaaaaatatc cttgcgataa cggttcaccc aagagctgtc
     5521 ggccaccggt tgtgcccccg ttccctcggc ggtagaagag ccaagtacta caatctggca
     5581 ggtcacgtcg tcatctgcaa tttcaaaagc agcatcgctt tggtcgctca gttcgttggc
     5641 cgaaagacgc cccagacaat cggcnnnnnn nnnnngaggg cgcattgggc accgtccagg
     5701 catattgttt ttgtacggca atcaccgtgt caatggcggt ccaggaggcg ccgttgtcgg
     5761 tagaatattc caaaatgacg ctgcccacat tcttgctctc ccagatgatg gaaggcgttt
     5821 ttcccacttg ccaaaactcg ccgccattgg gcgaaagcac gctcagttca ggttcgccgg
     5881 cttcttcttc atcataaatg actttcatgg cgccgagata gaagaaaccg gagctgttgt
     5941 tgttgttcgg gccaggcgcc acctctatgc gaacggtgcc gtcgttggcc ggaaacagcg
     6001 tggcagtggc tgtgttcgca gcgttgttgg aagcatccaa atacaccgtt tcagccgaca
     6061 aaccctgcaa ggtatagcgg gcttcgcgat tgtcggaagc gccggtgcga gaggcaaaaa
     6121 acacaaaggt atgcggtttg ccgggcgtca gattggtaaa ctctatgcca ccgacgggct
     6181 gcgtttgccc tccaaacagt acggtattgc cgaagaaact gtcgccggtg gccgttgccg
     6241 ggaatccaat ggccggatcg ggactaagcg taccggcagt gttgatgttg ttgaaggcat
     6301 cataaacgaa aaccgacttg ccggaggaga atccttctga agtgagcaga tcgtctatgc
     6361 ggcctgctac aggatctgcc acattgttcc agggcagcgg cgagaagttg ttgccaaaat
     6421 cgaggagcag ggtatcctga gcgggcggcg gcggcgggtt gcgttcatac accacgcgca
     6481 tggcgccgag gtagtagaaa ccggtcgagt tgttgttgtt ggggccgggc gccacatcaa
     6541 tgcgaatgat gccctgctca ttgggaaaca gtgtcgcgga ggctactcga tctgtgttgt
     6601 tggaagcgtc gaggtcggcg gtttcgatgg tttggccggt gaaggtatag cgagcctccc
     6661 ggttgtcggc cacgccggcg cgggaagcaa aaatcgtaaa cgtataaggc ttgtcgggca
     6721 agagattcga catttccaga cctgcggtgg gttgtacttg tccgccgaaa gtggccgtat
     6781 tgccgaagaa gctgtcgccg gtggccgttg ccgggaatcc gatggcaggg tcggggttgg
     6841 cggcgccggc agtgttgata ttgttgaacg catcgtaaat cacaatggaa gcgcccgaag
     6901 cattaccggc agaagtgaga atgtcttcaa tctgaccggc taccgggtcg gtgatgttgt
     6961 tccagggcgc gggagtgagg ctgttgccga agtcaatgag cagcgtgtca atgagtgaaa
     7021 tgaccggcgg ctcctcgaat tcatacgtca cccgaatggc gccgaggtgg taaaagccca
     7081 gcgaggtatt gttgttgggg ccgggcgatg ccgtgatgcg gatggtgctg tcggcggcgg
     7141 ggtaaatcgt ggccgtcacc accatatcgg tattggaaga agcattgagc gtaagcgtct
     7201 cagatgcagc gccctctatc agataggaag cttcgcgcat gtcggcgccg atgcgggatg
     7261 caaaaatggt gatcgtgtaa ggtttctccg gcaacaaatt gaacagcgct acgccaccgg
     7321 taggctgcac ctgacctgca aacggaccag tattgccata aaaactatcc ccggaagcgg
     7381 aggcaggaaa accaatggcc ggcgatggtg tctgggtacc ggcggtattc acgttgttga
     7441 aagcgtcaaa cacttcgatg cctctgcccg aaggtctgcc tgctgtgttg atgatgttgt
     7501 caatgcgccc gtccacgggg ttggtcaggt tgttccaggg cgagggcgag agtaccggcc
     7561 caaagtcaat aagcagggta tccaattcca tcacaacggg cggctcctcg tgttcgtaac
     7621 tcatggtcag cgtgccgagg tagtaaaatc ccgtgccgtt ggtgttgttt ggccccggcg
     7681 ctgcatcaat gcgaatgacg ccgccggcat cgggttggag cgtcacggtc accactcgat
     7741 ctgtgttgtt ggaagcatcc agatcggcgg tttcgatggt ctggccggtg aaggtgtaac
     7801 gtgcttcgcg gttatcggcc acgcccatgc gggatgcaaa aatggtgaag gtatgcggtt
     7861 tgcccacggt gaggccgccc aattctacgc cgccggtgag ttgcacttgt ccgccgaaag
     7921 tagccgtgtt gccgaagaag ctgtcgccgg tggcggtagc aggcaagccc agcgcggggt
     7981 cgggagcggt ggcgccgttg gtgttgatgt tgttgaacgg atcgtacacc aaaatggatt
     8041 taccggtcat cagccctgcc gtgttcgtca ggtcggcaat gaagccggcg gcggggtcgg
     8101 tgacgttgtt ccagggagcg agtgaggtat tggagccgcc aaagtccacc aaaatggtgt
     8161 ccatcggcat gggcggcggc ggagcttgct catacaccat cacaaggctg ccgaggtagt
     8221 aaaatcccgt gccgttggtg ttgttgggac ccggcgctgc gtcaatgcgg atgacgccgt
     8281 tggcatcagg ctggagcgta accgtcacca cccggttggt attgttggag gcatccagaa
     8341 aagctgtttc aacagtaaga ccggtgaagg tgtagcgcgc ttcgcggttg tcggctacgc
     8401 tcatgcgcga agcaaaaatg gtgaacgtgt gcggtatgcc cggcctcaga ccggccaatt
     8461 ctacgccgcc ggtgggttgc acttgtccgc cgaaagtagc cgtgttgccg aagaagctgt
     8521 cgccggtggc ggtagcaggc aagcccagcg cggggtcggg agcggtggcg ccgttggtgt
     8581 tgatgttgtt gaacggatcg tacaccaaaa tggacctgcc ggaaggctgg ccctgggtgt
     8641 tgagcagatc ggggatgctg ccggctaccg gatcagtgac gttgttccag ggcgccggcg
     8701 agaggttatt gccgaagtct atcagaatcg tatcgttctg agcaacaagg gcgtaccctg
     8761 caaaaaacag catcgcccaa aggagaagta catgtttttt catttgttga tagcttgtga
     8821 gttggttata aagtggttgt ttgtgcgcca gggacagcag cggtaccgac gtgcggctcc
     8881 gtagcatgga ggtttaaacc ccgacgctac ggagctgcat ggaggtaaga gcgaatggcc
     8941 cgccccgccg cccgatgcca cccgctttga gaatggccgg gggtggcagg cagatgcggg
     9001 gggcgccctg tatcaataat tcggat
//
LOCUS       DAORLF010000169         8990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4181_length_8990_cov_2.071517, whole genome shotgun sequence.
ACCESSION   DAORLF010000169 DAORLF010000000
VERSION     DAORLF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_8990_cov_2.071517"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    330..345
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<346..2499)
                     /locus_tag="PK198_13790"
     CDS             complement(<346..2499)
                     /locus_tag="PK198_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39856.1"
                     /translation="MLINYLNIAVRQLLRHKRFSALNIFGMAAGMSVCMLVIMAVRDQ
                     YGYDDFHANGERIYRIISAEADKNQPLQKATHATAPLSLLEVIQDHSPYMEAGARLVS
                     MGDDFKIGERVFPNELGGYAVDQGFLDMFSFGWEAGNHSDALTQPRSVVLTKTTARRL
                     FSGSPAIGMTVQLGEMGDFLITGILPDPPKRSHIRFDYLISYPTLQSMSAEELSKLGI
                     NGFDEIWRGLTYVLLTEKGSRAMLDQTLASQTASYSVRSEKMHYIFQSQALMDVMPSR
                     DLANEIGVGTPHVVLYFLMALGLVILLAACFNYVSLSLARSIKRAKEIGVRKVFGAAK
                     KDVVRQFLTEAVLISLLAFVLAVGFLELLIPAFYNLDPFVSTIFQLERSFSNYLIFLL
                     MSLLVGLAAGVFPALHIAGFQPLQAIQQLKNVRLFSRMGLRKTLVTLQLTLSLVFILV
                     VIIVLKQQNHVLNADLGQNIENIVSVQLQGLSFDKFSQRAMQVKGVESVSSTSVAILA
                     GENNEETAIFGERNDSMQVGQSFISQNFLQNMSIPLVAGVNFPEDNYSEGEQFAIVNE
                     TATRQMGFPKPDAALGSSIVASGVPLSIIGVTKDFHHDNIWFSPIKPFVLRNKSERAY
                     NAYLRLSEANQRETLASLHAIWSELSPQKNWYGFYLEERVYNMAKFFRMGSSIIGFVG
                     FLTIVIACLGLLGMVVYVVEGSVKEVGIRKVLGAGE"
     gene            complement(2610..>3932)
                     /locus_tag="PK198_13795"
     CDS             complement(2610..>3932)
                     /locus_tag="PK198_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF39857.1"
                     /translation="PLLKHISDVPVEAPFLSLPQTWAFLVFVTIAVTLLAGFYPAVVL
                     AGFEPAGALKNSVGAVRGGHRAWVRQSLVVLQFSIAIAFIVGTIITLSQLHFIRTKDL
                     GFDQNLIYTFYYNNDSLSVAKLNTLKNRLLQAPEVEAVSYNTDPPSSGNSWMSNFAFP
                     SNAEDADFALQIKYADTDYPKTFGLRMAAGKWYDPSDTMKQAVVNEAFLRKMGVEKPE
                     DAIGEDLKLGGRAGIIITGVVQDFHAFSVHQPIAPLMISTQRDYYYNAAVKVNPGDMA
                     AAARAIESAFDEVYPEQVFEGHWYDETIARFYEDENRFANACKGFGVLAIFIACIGLF
                     GLSSHAAARRAKEIGIRKVLGAGTAGITALLARDFIKLVLVAFVLAAPAGWYFMNRWL
                     QDFAYRIDIQWWVFVVAGAVALVGATLVVALQTVRAALADPVESLRSE"
     assembly_gap    3933..3942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3943..>4747)
                     /locus_tag="PK198_13800"
     CDS             complement(<3943..>4747)
                     /locus_tag="PK198_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39858.1"
                     /translation="SVPGAEDGTPPKKFLRKDPSVAFFTEPSFFEIFDLEWLAGDPKT
                     ALRDEGSVVIIRSEAERFFGSWQAAMNETLVLDNVARVTVRGVVEDLPPDCDLPINML
                     ASWPTYIAQQALYYYDDNWGSCNSSNQVFGLLADASQLGAAERSLAKVGEKQYAENSS
                     TGKADKTHRLQPMSDLHFSDKLNNSAFRTITKGRLKVLSFIALLILGMACFNFINLST
                     AMASQRAKEIGVRKTLGVGRGQLLRQFLGETAVVSVLSVALGVAVARLCL"
     assembly_gap    4748..4886
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5076..5765)
                     /locus_tag="PK198_13805"
     CDS             complement(5076..5765)
                     /locus_tag="PK198_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF39859.1"
                     /translation="MLSIKNIERFYKTGPLTTWVLRDINLKVSEGEFVTIMGPSGSGK
                     STLLNILGMLDQPDAGEYLFFDEPVHRFNEKRRSELHKHYIGFVFQAYHLIDELTVYE
                     NIETPLLYRGIKSAERKAMIADVLDRFNMVAKKDLFPAQLSGGQQQLVGVARAIIGKP
                     KLLLADEPTGNLHSSQGETIMELFAQLHREGMTIIQVTHNEKNAQYGTRLIQLEDGFV
                     RDDAGVARLNV"
     gene            complement(5857..6543)
                     /locus_tag="PK198_13810"
     CDS             complement(5857..6543)
                     /locus_tag="PK198_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF39860.1"
                     /translation="MIRTNKLVKVYRTDEVQTTALNAVDVSIAEGEFVAVMGPSGCGK
                     STLLNVLGMIDSPSSGEYFFNGEEIGKLPERKRSDVRKHNLGFVFQSFNLIDELTVYE
                     NIELPMLYTKVPAAQRKKRVEELMEGMNIMHRRNHFPQQLSGGQQQRVAVARAVVNKT
                     KLILADEPTGNLDSQNGDEVMKILADLNAEGTTILMVTHSQYCAEFGNRIIRMLDGQV
                     VTENVIRQYL"
     assembly_gap    6672..6794
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6816..8063)
                     /locus_tag="PK198_13815"
     CDS             complement(6816..8063)
                     /locus_tag="PK198_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF39861.1"
                     /translation="MDQKIEKRTWTWQRIAILLIGLGAAAWLATALLRDTGGAKLNVA
                     SERLLLDTVRRGEFQEFIPVTGIALPIRNVVLAANEGGKVEERFVEDGAMVTAGQAIL
                     RLSNTDLQLNYLNQEGSIVNQINQIQNMSLLRDQQSLNLRETLLDIEYRIGLTGQRLQ
                     RNKTLQQGGAVSKVEVDEMQEDYNNLLRRKSLLLKTIQKDSLSALIQERQMENSLDLM
                     RRNLDIAKQSLENLVVKAPIEGQLSGLNTELGESISRGVQIAQIDDLSNFKIRVRIDE
                     FYISRVFPDQVGTFTFAGKQYTLKIKKIYPQVVGGNFEADMLFVGEFPAAIKRGQTLS
                     VKLELSAEEQATLLARGGFYQKTAGRWGYVLNKEGNAVKKSVQLGRQNPNFYEVLSGL
                     QPGDVVITSGYDAFGDKDVLLLK"
     gene            complement(8357..8986)
                     /locus_tag="PK198_13820"
     CDS             complement(8357..8986)
                     /locus_tag="PK198_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HRF39862.1"
                     /translation="MLYGTAKVIVEHIAEGQPYSKVKKVYSVNIVYFDLGQGDDYVHH
                     GTTTFIGIHSNHELELSEKQKELFKKTSVSDLYPEYYVIKTNRFNEVAKDTLDEWIYF
                     LKTAEIKDEFTAKGLKAAGQKLDILKLAPADRKEYDRYMEAQRSDASFVETVNLEITT
                     AVNEALKSRDEEIAENILRKGATIEFAAEITGLSIVEVQGIAARLGLDK"
BASE COUNT         2006 a         2396 c         2220 g         2080 t
ORIGIN      
        1 tacttatcta atcctttgac gaaaatgacc actttatttt tttattgccc cttgtcgctc
       61 ctcaaacgcg actatcaatc atacagatag ccgcctgcgc tattcattct taatcgcctc
      121 aatggggttt gccctcgctg cccaatacgt ttgtgacatt accgtgagta accccaaaaa
      181 caacacaatg gagatgccaa gcgccaccat ttccacttcc acctaaatgc gcatcagaaa
      241 attgttcagc cacatatcgg cggcaaatac cgtgagcggt acggcgatga aaacagcgat
      301 gcccagtgca cgacatgcac cagtgcaggn nnnnnnnnnn nnnnnctcgc cggcccccag
      361 cactttgcga atgcccactt ctttcacgct tccctccacc acatatacca ccatgcccag
      421 cagtcccaag caagcgatga cgatggtgag aaagcccaca aatccaatga tacttgaacc
      481 catgcgaaaa aatttggcca tgttgtaaac ccgctcttcg agataaaagc cgtaccagtt
      541 tttttgtggc gacaactcag accaaatcgc gtgcaaagag gcaagggttt cgcgttggtt
      601 ggcctcgctc aggcgtaaat atgcgttgta ggcgcgctcg cttttgttgc gcaaaacgaa
      661 gggtttgatg ggggaaaacc aaatgttgtc gtggtgaaaa tccttcgtta caccaatgat
      721 ggacagcggt acgccactcg ccacaatgga actgccaaga gccgcatcgg gctttggaaa
      781 gcccatttgt cgggtggcgg tttcgttcac aatggcaaac tgctccccct cagagtagtt
      841 gtcttccggg aaattgacac ccgccaccaa cgggatgctc atattttgca gaaagttctg
      901 actgatgaat gactgcccta cctgcatgga gtcgttgcgc tcgccgaata ttgccgtttc
      961 ttcattgttt tcacccgcga gtatggcaac actggtggac gaaaccgatt ccactccctt
     1021 tacctgcatt gctctttggg aaaatttgtc gaaagaaaga ccctgcaact gcacgctgac
     1081 gatgttttca atgttttgcc ccaagtctgc attcagtaca tggttctgtt gtttcaagac
     1141 aataatgaca acgaggatga acaccagcga cagcgtaagt tgtaacgtca ccagggtttt
     1201 acgcaatccc attcgggaaa ataaacgcac gtttttgagt tgctgaatgg cctgcaatgg
     1261 ttgaaatccg gcaatatgga gcgcaggaaa aacgcctgcc gccagtccca ccaacaggct
     1321 cattaataag aaaatcagat agttggaaaa agatctttcc aattgaaaaa tggtagatac
     1381 gaaggggtcg aggttataaa atgccggtat caacagctct aaaaatccga ccgccagcac
     1441 aaacgccagc aaggaaatca gtacggcttc tgtcaaaaac tggcggacaa catctttttt
     1501 tgcggccccg aaaaccttgc gaacgccaat ttctttggcc cgtttgatgc tgcgggccag
     1561 cgacaggctc acataattga aacaagctgc cagtaagatg accagcccca atgccatcag
     1621 gaaatacaac accacatgcg gcgttcccac tccgatttcg ttggccaaat ctcggctggg
     1681 catcacgtcc atcagggctt gcgactgaaa aatatagtgc attttttcgc tgcggacgct
     1741 gtatgatgcc gtttgagagg cgagcgtttg atcgagcatt gcacggctgc ctttttccgt
     1801 caacaaaaca taagtcagtc cccgccatat ttcatcaaaa ccgttgatgc ccaacttcga
     1861 taattcctcg gcactcatag attgcaaagt cgggtaggat atcaaataat cgaatcggat
     1921 atgcgagcgc ttcggcgggt cgggcagtat gccggtgatc aaaaaatcgc ccatttcgcc
     1981 caattgcacc gtcattccaa tggctgggct gcccgaaaac agccgccggg cggtggtttt
     2041 cgtcaaaacg accgatcggg gttgggtcag cgcatcagag tggttgcctg cttcccaacc
     2101 gaaactgaac atatcaagaa agccttgatc caccgcgtaa ccgccgagtt cgttggggaa
     2161 tacccgttcg ccgattttga aatcatctcc catcgaaaca aggcgggcgc ccgcttccat
     2221 gtaaggcgaa tggtcctgta tgacttcgag caaagacaaa ggcgcagtgg cgtgagttgc
     2281 cttttgcagc ggctggtttt tatcagcttc cgccgagatg atccggtaaa ttcgttcgcc
     2341 attggcatga aaatcatcgt agccatattg atcgcgtacc gccatgatga ccagcataca
     2401 cacagacata cctgcggcca tcccgaagat gttgagggca gagaatcgct tgtggcgaag
     2461 gagttggcga acggcaatgt ttaaataatt gatcaacatg gcaatggtga tttaaggaat
     2521 gaagtatcgg caactttagc taccgaaaaa atgcagtaca gccggagcga aaatgcagcg
     2581 caggttttga tcggcgactg aagccactgc tactcgcttc tcaacgactc caccgggtcg
     2641 gccagcgcgg cgcgcaccgt ttgcagggcg accacgaggg tcgccccaac gagggccacc
     2701 gcacctgcca cgacgaacac ccaccactga atatcaatgc gatacgcgaa atcttgcagc
     2761 cagcgattca taaaatacca gcctgccggc gccgcaagga cgaaagccac caacaccaac
     2821 ttgatgaaat cgcgggccag cagcgcggtg atgcctgccg tgcccgcccc cagtactttc
     2881 cgaatgccga tttctttggc cctgcgcgcc gccgcatgag acgacaagcc aaacaaaccg
     2941 atgcaggcaa taaatatggc cagcacgcca aaacccttgc aggcattggc aaaccggttt
     3001 tcgtcttcgt aaaaacgagc aatggtttcg tcgtaccagt gcccctcaaa aacctgttcg
     3061 ggatacacct cgtcaaaggc gctctcaatg gctctggcgg cagccgccat atcgccggga
     3121 ttcaccttca ccgcagcatt gtaatagtaa tcgcgctgcg tgctgatcat aagcggcgcg
     3181 atgggctgat gcaccgaaaa agcatgaaaa tcctgcacca ccccggtgat gattatgccc
     3241 gcacgaccgc caagtttgag gtcctcgccg atggcgtcct ctggtttttc tactcccatt
     3301 tttctcaaaa acgcctcatt cacaacagct tgcttcatcg tatcgctggg gtcgtaccac
     3361 ttgccggcgg ccatgcgcag gccgaaggtt ttcgggtagt cggtatcggc gtacttgatt
     3421 tgcaaagcga agtcggcatc ctcggcattg gaaggaaaag caaaattcga catccaagaa
     3481 ttgcccgatg aaggcgggtc ggtattgtag ctcaccgctt ccacttccgg cgcttgcagc
     3541 agcctgtttt tcagtgtgtt gagttttgct acactcagac tgtcgttgtt gtaataaaag
     3601 gtgtaaatca gattttggtc aaaacccagg tctttggttc tgatgaaatg cagttgcgac
     3661 agcgtgatga tggtgcccac gatgaaagcg atggcgatgg aaaattgcag caccaccaag
     3721 ctctgccgca cccaggcccg atgcccgccg cgcacggcgc ccacgctgtt tttgagcgcg
     3781 ccggccggtt cgaatcccgc caaaaccacc gccggataaa accccgccaa cagtgtgacg
     3841 gctattgtga caaacaccaa aaacgcccag gtctgaggca aactcaaaaa cggggcctca
     3901 acgggcacgt ccgaaatatg cttcagcaag ggnnnnnnnn nncaggcaca acctggccac
     3961 ggccactccc agtgccaccg acaaaaccga caccactgcc gtttcgccca ggaactgccg
     4021 cagcaattgc ccgcgcccta cgcccagcgt tttgcgcacg ccgatctcct tggcccgctg
     4081 cgaggccatg gcggtagaga gattgatgaa gttgaaacag gccattccaa gaatcagtaa
     4141 agcaatgaaa gacagtactt tgagtcgccc tttggtgatg gtgcggaagg ccgagttgtt
     4201 cagtttgtcg ctaaagtgca ggtcggacat gggctgtagg cgatgcgttt tgtcggcttt
     4261 tccggtgctg ctgttttcgg catattgctt ttcgcccact ttggccagcg agcgctcggc
     4321 ggcacccaat tgcgaggcat cggccagcag gccaaacacc tgattgctgc tgttgcaact
     4381 gccccaattg tcatcgtagt agtaaagggc ttgctgggca atgtaagtgg gccacgatgc
     4441 gagcatattg atgggcaggt cgcaatcggg cggcaggtct tccaccacgc cgcgcaccgt
     4501 cacccgcgcc acattgtcga gcaccagtgt ttcgttcatg gcggcttgcc aggaaccgaa
     4561 aaatcgctcg gcctcggagc gaatgatgac cacggagcct tcatcgcgga gagccgtttt
     4621 ggggtcgccg gccagccact ccaagtcaaa aatttcaaaa aacgaaggct ccgtgaaaaa
     4681 agccacgctg gggtcttttc tcaaaaattt cttcggcggc gtaccgtctt cggctcccgg
     4741 cacagagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnngcgt caccacgcgc acgatgcggt cgtagcggct
     4921 gaagttttta ttgtagccca attcgtatcg caccacctga aaaataagga gcacggcggc
     4981 aatacccagc cccagtccca gaatgttgat gccggcatac accttgttgc gggccatcga
     5041 acgcagaatg aatttgaatt gcatctcttt gaattttaaa catttaacct tgccacccct
     5101 gcatcatctc gcacaaaccc atcctccaat tggatcaatc gcgtgccgta ttgcgcattt
     5161 ttttcgttgt gcgtcacctg aatgatggtc atgccctcgc ggtgcaattg ggcaaacaac
     5221 tccataatcg tctcgccctg agaagaatgc aggttgcccg tcggttcgtc ggccagcagc
     5281 agtttgggtt tgccgatgat ggcccgcgcc acgcctacga gttgttgctg cccgcccgac
     5341 aactgcgccg gaaacaggtc ttttttggcc accatgttga atcggtcgag cacatcggcg
     5401 atcatggctt tgcgttcagc cgacttgatg ccccggtaca gcagcggcgt ttcgatgttt
     5461 tcatacaccg tcagctcgtc aatgaggtgg tacgcctgaa acacaaaacc gatgtagtgc
     5521 ttgtgcagct ccgagcgccg tttctcattg aaccgatgca ccggctcgtc gaagaaaagg
     5581 tactcgcccg catccggctg atcgagcatg ccgaggatgt tgagcagcgt ggatttgccc
     5641 gatccgctcg gccccatgat ggtcacaaac tcgccctccg acaccttcag gttgatgtcg
     5701 cgcagtaccc aggtggtcag cggtccggtt ttgtaaaacc gctctatatt cttgattgac
     5761 agcatcttgt tttttatttt tcaaaccctg aaacccaact cgcagctcgt gccatgcctg
     5821 tggcgtggtc gcagcttaca gccgctccca cctgcctcac aaatactgcc tgatcacatt
     5881 ctccgtcacc acctgcccgt cgagcatgcg gatgatgcgg ttgccgaact ccgcgcaata
     5941 ctgcgagtgc gtcaccatga ggatggtcgt accctcggcg ttcaggtcgg ccaagatttt
     6001 catcacttcg tcgccgtttt ggctgtccag attgcccgta ggttcgtcgg cgaggatgag
     6061 tttggtcttg ttcaccacgg cccgcgccac agctacgcgc tgttgttgcc cgccggagag
     6121 ctgctgcgga aagtgattgc gccggtgcat gatgttcatg ccttccatga gttcttccac
     6181 gcgctttttg cgctgggcag ccggcacttt cgtgtagagc atcggcaatt caatgttctc
     6241 atacacagtg agttcgtcta tgagattgaa gctctgaaag acgaagccga gattgtgttt
     6301 gcgcacgtcg gagcgcttgc gttcgggcag tttgccgatc tcttcgccgt tgaaaaaata
     6361 ctcgccggaa ctcgggctgt ctatcatgcc cagcacattg agcagcgtgg acttgccgca
     6421 acccgagggg cccatcacgg ccacaaactc gccctcggcg atggacacgt ccacggcatt
     6481 gagcgcagtg gtttgcacct catccgtgcg gtaaactttg actaatttgt tggttcgtat
     6541 cattttatgc ttgttttttt atcaattgtc ccaacctgaa accacgactg tcgtcgcggc
     6601 gcggcgcgga atttttgaac gcgaataatg cgaatcagcg cgaatttccg cgaacgagag
     6661 ggaaacccga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnntcgaaa ccttgaactc gaaacctact tcaacaggag cacatcctta
     6841 tccccaaaag catcgtagcc cgaagtaatg accacatcgc ccggctgcaa gccgctgagt
     6901 acctcgtaga aattgggatt ttgccgaccc aactgcaccg attttttcac cgcattgccc
     6961 tccttgttca gcacataccc ccagcggccc gccgttttct ggtaaaaacc gccccgcgcc
     7021 agcaaagtgg cctgttcttc ggcgctcaat tccagcttca ccgagagcgt ttgtccgcgt
     7081 ttgatggccg ccggaaattc ccccacgaac aacatgtccg cctcgaaatt gccgcccact
     7141 acctgcgggt atatcttctt gattttgagc gtgtattgct tcccggcaaa cgtaaatgtg
     7201 cccacctgat cggggaatac ccgcgagata taaaactcgt ctatccgcac ccggattttg
     7261 aaattgctca ggtcgtctat ctgcgctatc tgcacgcccc gactgatgga ctcgcccagt
     7321 tcggtgttca agccggagag ctgcccttcg atgggcgctt tcaccacgag attttcaagg
     7381 ctctgtttgg cgatgtccaa gttgcggcgc atcaggtcca gcgagttttc catttgccgt
     7441 tcctggatga gcgccgagag gctgtctttt tgtatggttt tgagcaacag cgatttgcgc
     7501 cgaagcaggt tgttgtagtc ttcctgcatt tcgtccactt ccactttcga tacggcgccg
     7561 ccctgttgca gggttttgtt gcgttgcagg cgttggccgg tcagtccgat gcggtactcg
     7621 atgtcgagca gggtttcgcg gagattgagg ctctgctgat cgcggagcag gctcatgttc
     7681 tggatttggt tgatctgatt gacgatgctg ccttcctgat tgaggtagtt cagttggagg
     7741 tcggtattgc tgaggcgcaa aatcgcttgt ccggcagtca ccatagcgcc gtcttccaca
     7801 aaacgctcct ccaccttgcc gccctcattg gccgccagca ccacgttgcg aatgggcagc
     7861 gcaatgccgg taacgggaat gaattcctga aattcgccgc gccgcacggt gtccaacagg
     7921 aggcgttcgg aggccacgtt gagtttggcg ccgccggtat cgcgcagcag ggccgttgcc
     7981 agccaggccg ctgctccgag gccgatgaga aggatggcaa tgcgttgcca ggtccaggta
     8041 cgtttttcaa ttttctgatc catgctgttg atatgtcaga tgttgtgcca aaaatgcaaa
     8101 catctgaaaa acagcggaat aaaaaaacgg ccccttcaaa aaagtgttcg attccggaca
     8161 gcaggtgtgc ggttttgcgg acaatggcgc gagttttggg gcgggcgaga gggtttatga
     8221 gacaacgaaa tagtggtagc ggggacggag gtcatgctac cagcgatggt gtttttttag
     8281 attatgtgac gaacggcgcc gctgccgaag tgcccacgtt taggaggcat gacaggcggt
     8341 ggattgttgg gcgattttat ttgtccaaac caagtcttgc agcaattccc tgaacttcta
     8401 caatggaaag ccccgtgatt tcagcggcaa attcaatggt tgctccttta cgcaatatgt
     8461 tttcggcaat ttcttcatct ctacttttca gggcctcatt taccgccgtt gtaatttcta
     8521 agttcaccgt ctccacaaaa ctcgcatcgc tgcgttgcgc ttccatgtaa cggtcatatt
     8581 cctttcggtc ggcgggtgct aatttcagaa tgtcgagttt ttggccggcg gctttcaagc
     8641 cttttgcggt gaattcgtct ttaatttcag ccgttttcag gaagtatatc cactcatcaa
     8701 gcgtatcctt ggcaacttca ttgaagcggt tggttttgat cacatagtat tccgggtaca
     8761 agtcagatac agaagtcttt ttgaaaagtt ctttctgctt ttcggacagt tcaagctcgt
     8821 gattgctgtg gatgccgata aatgtagtgg tgccgtgatg cacataatca tccccttgac
     8881 cgagatcaaa atagacgatg ttcacagaat acactttttt gactttggag tatggctgtc
     8941 cctcggcgat atgttccaca atgactttgg ccgtgccgta aagcattcgc
//
LOCUS       DAORLF010000170         8975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4189_length_8975_cov_2.195067, whole genome shotgun sequence.
ACCESSION   DAORLF010000170 DAORLF010000000
VERSION     DAORLF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4189_length_8975_cov_2.195067"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK198_13825"
     CDS             complement(<1..260)
                     /locus_tag="PK198_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRF39863.1"
                     /translation="MKRFWLMVPALMCSIAMYAQQIQEGVASVFDDSFQGKETRYGVK
                     YDKRELTCAHNIHQFGSLLRVTHLENKRSVLVRVIDEGPFIKG"
     assembly_gap    771..802
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<803..2557)
                     /locus_tag="PK198_13830"
     CDS             complement(<803..2557)
                     /locus_tag="PK198_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39864.1"
                     /translation="MKCSAISIHIYRRQSNLLFILLLGITLCSVPNLLAAQPVEICSG
                     SPDNYLCPEEPVSPDPALLGLFSAQNAANPCFGLLAFDNFPGQTKCNGTDIPSNAYFI
                     HQLTFPVSSGNVITATLQFRAKAAPDPNGQTHTDFIAFFEGATYITGANLSQLPGANG
                     TWNPNQDAMFTLQLGNLPAAFSVNNILQHLNDGDLDIVIGNETGVDWMCISPTSTSPQ
                     VSTRYEIQRPRCKYTLIPPRARWLISMRSVDSGGRDSVSSSPYHLQTAVLKPTAFPAP
                     NVLFTDSLGNPVFTSFGEGIMLFEDNAVAAKIQGISPAPPGYEWYGLQSNAITPSDSP
                     NPDSAYFDIAFDWTDLPPGSEDSTGAFWMSDDKDDLYQELITDAFTTLDTSRLAAKTD
                     YGIEVKNCVYQSDPFYVKWNIYWWCHDYSNRISVFSMPYHLQTAVLKPVVFPAPIVTL
                     TDFSGNPVQTAFGEQIILSESSSVAAKIQGLSPAPPGYEWYGLQSNSLTPSESPTQSS
                     QDFKIDFLWSTLPMGSQDSTGAFWVSDNKDDLYDEFITDAFTLLDAAQLTAQKEIGKN
                     NTLRLFPNPTPGQFTLELP"
     gene            complement(2595..>2955)
                     /locus_tag="PK198_13835"
     CDS             complement(2595..>2955)
                     /locus_tag="PK198_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39865.1"
                     /translation="YTAGALPRYSNHYTFIQSTADHYSGSIGSFPNFPDYEKTYRFGA
                     PNCEESRVVTSRDVYDVCGVNASKLQQVEYVRGKSITFKIFKRTYTRWIWKRKYHLLK
                     DYKSKNQLDYLYESVLK"
     assembly_gap    2956..3024
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3025..5265)
                     /locus_tag="PK198_13840"
     CDS             complement(<3025..5265)
                     /locus_tag="PK198_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF39866.1"
                     /translation="MLSATDNCSTPQIQYQVTGATSATGQNDASGLTFNTGVSTITYT
                     ATDNCGNVSSCSFDVTVLCEEPCPGNIIENSNFSVGAIAGPMPLQGSMANWQAAYGTP
                     VVSTGFGCGDPGYVELQGNRTQGSAIYQQLTTPIQKGKVYEMTVCVKSVSCSGCWGCP
                     VPYIKLRGIAFNGTLPTSGRHPAPGIDTAIIGVSGSIAVCDDWTQFVFHRWRAPKNYN
                     NISISIENNDPIGSDGISLGYIDNICFREVNDSIPCYLADLDSLGNVIPPFGLIDPTC
                     PVLEEEIDILMGNVNDIYGYCNPAPNDLDTWYEFCQDSCESIGGEFPDELLDFIENDS
                     LDNFMQDSIGVSTDSLLSDLSSFMDSLELALPNANMLDSIMGVGALAFNCRNLPPQGS
                     PPDDPNSPFNGRDIIFVHGLRTDPLMERLNGIPDALTTWPANRNEFYNGYWKRGAYQY
                     WNTHTAKYLNTWVDPNSFNNVIRSGNYSNRYIVVAHPATQTFVAGAHAVIEQIANAMI
                     DGVGVVNCDPTESRPANTFGHNGFIIISHSDGAPLSDIALTVSDLSGVPPLNVLLGDV
                     SFIAERCELHIALQGAFGGSNYASLALMGASTGLQYVKPLAEAFLGFEVNQPTDWLFR
                     SQLLDMSITRHLWGSLMNQVPVCVLTLAGGHPTDFGTDTGEKRILSQLVKHGIHHGFD
                     DGVLSIESQTASSGPRLTYPNRFQPIPGISPMLNAKLFDMGIHRDRAIRYYFDQKLDI
                     AQLHFQN"
     assembly_gap    5321..5426
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5427..>6481)
                     /locus_tag="PK198_13845"
     CDS             complement(<5427..>6481)
                     /locus_tag="PK198_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39867.1"
                     /translation="PNGDLLLAGNWGTSGAIFRADNAGAIIGGVSPEGIQMTFADVAQ
                     AGSNFIAAGFQFPSFSTRLMKFDPDLITLWQVSLPGLTAIRNVWTSGSSIFVSGSATV
                     EGFNRGVVLKFQDGVNGPVLQWMQYLQVNETSYTGGTAWPVPAGMAFCDGRNTTEERA
                     FMSVSDLEMNTCMTQTSTTTVTNTALTFNGPCCLNIDFTDALPGMDVASSLVTWQQAE
                     ACSLPCEASITVNYIDNCGHVQLTAVGSGLQPFSYQWCSGESTATIDRILSCGSHDFC
                     VSITCADGTVAVATQTINITDNIPPTALCQPGQGYELNANCTLPVTVAMINAGSFDNC
                     QIQSMSVSPATVQGCGV"
     assembly_gap    6482..6500
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6501..7110)
                     /locus_tag="PK198_13850"
     CDS             complement(<6501..7110)
                     /locus_tag="PK198_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39868.1"
                     /translation="MNLRYTCAVSITAIISIFPAFQPLQAQFQRQYGTALDESFSKVI
                     PHGSDYYVLGQAEATAGQQPRATVSRINSTGQLLWTRRLDVASQWNDAVLTPSGNLFL
                     VGHTLPLDVNSQSLMGVITPAGTFNWLRNYNATGRESFNRVVRNPVPNNAASPYYVLG
                     FQQEPSAVLNWDDVVLLTVSEAGNIGWKKIYSGAFGSTDDEFV"
     gene            complement(7306..7719)
                     /locus_tag="PK198_13855"
     CDS             complement(7306..7719)
                     /locus_tag="PK198_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39869.1"
                     /translation="MGKKILFGTLAGAVTGTVAAMAIFMGLLGSMAEKWYAENSACVK
                     SMEDAPMWAMIVASLVQGLLLAVLLYRFGVSTFKGGLIAGGWVTLLMVLWYGLFTYST
                     FNAYTLSWLPIDVIGNTICGALAGGVIGWVYGKVK"
     gene            complement(7767..8315)
                     /locus_tag="PK198_13860"
     CDS             complement(7767..8315)
                     /locus_tag="PK198_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF39870.1"
                     /translation="MHFQYPHTIENGAGERLTFKRLVQDPTGDWLEVENEVAPNCGPP
                     MHVHHKQAESLTVVQGTMGIEILGQAPLSVGPGETAVFEAGIPHRFWNAGTEPLICSG
                     WVKPAHNFEYFLTGIYDSMKENGGKQPGAFDGAFLLGRYRSEFDMLDIPGFVKKMIFP
                     IALFFGKLQGKHQKFEGAPEPL"
     gene            complement(8351..>8975)
                     /locus_tag="PK198_13865"
     CDS             complement(8351..>8975)
                     /locus_tag="PK198_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39871.1"
                     /translation="TLIFVAFNWDFDIAFNPMKAIAFEPDPSTALRWGWILDIFGYYL
                     PLAPALLVLHRWLSAQAPLQSQLFSLCGIGFIFIGATGAAMLAGATEPLYDAYRVGSE
                     ADKAVAAQTFANLNNEVMSGIWNLFSMTLAAIWWLGMGRLMRPAHKGLALFTQLLGLA
                     ALTDVLGYIFQSELVSGIGLNFYLFAAPLWAIWLGFYLWRNSLKITH"
BASE COUNT         2249 a         2136 c         2239 g         2125 t
ORIGIN      
        1 cctttgatga acggcccttc gtcaatgacg cgcaccagca ccgagcgttt gttttccaaa
       61 tgcgtcactc tgagcagaga gccgaattgg tggatgttgt gagcgcaggt cagctcgcgt
      121 ttgtcgtatt tgacgccgta gcgggtttcc ttgccctgaa aggaatcatc aaaaacagaa
      181 gccacgcctt cctgaatttg ctgcgcatac atcgctatgc tgcacatcag ggcgggcacc
      241 atgagccaga atcgtttcat aatggatggt tttgagggga gtaaaggtag aaacttgcgg
      301 ggcaatcgtc caaaaaaatg cagaaatgat ggtttcggct gagtggcgat aaaaattttg
      361 cctcgccact cagcggagtg ttgaataaaa tgaagtacag cattaaaaat ctgatccttt
      421 ctaaaaacca aactactcag cgtgtcctga ccgcataccc agacgccgtc ggggattcgg
      481 gatagagtac gcggcgggtg gcaggcgctg caagcgaggg aaagaaggca tggaatgaaa
      541 tacaggctcg aaaaccctcc aattctgaaa attctatcat cctgtgaatt ctgattcaga
      601 cacttttcat tgcctcacca ctcttcctac gcccactacg ctcccctcct gccacacctg
      661 cacgaagtac atacccgccg gcagggcgct caggtcgaac acctggagcc tgctacccgc
      721 ttcggcccgc tgttgcagca gcgtttgccc ggtgatgccg gcgatgcgga nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nngggtagtt cgagggtgaa ctggccggga gtggggttgg
      841 ggaagaggcg gagggtattg ttttttccta tttccttttg agcggttaat tgagcggcat
      901 ctaataaggt aaacgcatcg gtaataaatt catcgtacag atcatccttg ttatcactta
      961 cccaaaatgc ccccgtcgaa tcttggctgc ccatcggtaa agtcgaccat agaaaatcaa
     1021 tcttgaagtc ttggctgctc tgggttggag attctgaggg cgtaagcgaa ttcgattgaa
     1081 gcccatacca ttcatatccg gggggcgccg gagataatcc ttgtatttta gcagccacag
     1141 agctgctctc tgagagaatg atttgctcac caaaagcagt ttgcacgggg ttgcccgaaa
     1201 aatcagttag tgtcacaatt ggtgcgggga aaacgaccgg ttttagaacc gccgtttgaa
     1261 gatgatacgg catagaaaac actgaaatcc gattgctgta atcatggcac caccaatata
     1321 tattccattt tacataaaac ggatccgatt gataaacaca gtttttcacc tcaataccgt
     1381 agtcggtttt tgccgccaat cgtgaagtat ccagtgtggt gaatgcatcg gtaataagtt
     1441 cttgatacag gtcgtcttta tcatcgctca tccaaaaagc ccctgtcgaa tcttcactgc
     1501 ccggcggcag gtctgtccag tcaaatgcaa tgtcaaaata tgcgctgtct ggattgggcg
     1561 agtccgaagg agtaattgca ttcgattgaa gcccgtacca ttcatatccg ggaggcgctg
     1621 gtgatattcc ctgaattttg gcggctacag cgttgtcttc aaaaagcatt attccctcgc
     1681 cgaaagaagt aaaaacagga ttgccaagag aatccgtaaa taaaacgttc ggtgcaggga
     1741 aggcggttgg cttcaaaacg gccgtttgaa gatgatatgg acttgaagac accgaatctc
     1801 gtccacctga atcaacggac ctcatagata ttaaccacct cgctctcgga gggatcagag
     1861 tatatttgca tcttgggcgc tggatttcat atctggtcga tacttggggc gatgttgagg
     1921 tgggggaaat acacatccaa tctacgccgg tttcattgcc gatgacaatg tctaagtctc
     1981 cgtcgttgag atgctgtaag atattgttga cggagaaggc agcaggcagg ttgccgagtt
     2041 gcagggtgaa catggcgtcc tgattgggat tccacgtgcc gttggcgcct ggtaattggc
     2101 tcaggttggc gccggtaatg tatgtggcgc cttcaaaaaa ggcaatgaaa tcggtatggg
     2161 tttggccgtt tggatcaggc gctgctttgg cgcgaaactg gagggtggca gtaatgacat
     2221 ttcccgacga taccggaaag gtaagctgat ggatgaaata ggcattggaa ggaatatccg
     2281 ttccgttgca tttggtctgc cccgggaagt tgtcgaacgc aagtaacccg aaacaagggt
     2341 tggcagcatt ttgggcgctg aataaaccaa gaagtgccgg gtctggagaa accggttctt
     2401 caggacaaag ataattatcg ggcgagccgg agcatatctc taccggttgg gcggctaata
     2461 gatttggaac gctgcacaag gtaatcccca ataggagaat gaataacagg ttactctgcc
     2521 tcctatatat gtgtatggat atcgctgaac atttcatggt ggatcttttt gaatttgaaa
     2581 aaaattgtgg cccgctactt cagcacactt tcgtacaaat agtccaactg gttcttgctt
     2641 ttataatcct ttagcaagtg gtattttcgt ttccaaatcc accgggtgta tgtccttttg
     2701 aatattttaa aagttattga cttaccacgg acatactcga cttgctgcaa cttggaagca
     2761 ttgacgccgc acacgtcata cacatccctt gaagtgacta cccgggattc ctcgcagttg
     2821 ggagcgccaa aacgatatgt tttttcataa tcggggaaat tggggaaaga cccgatagaa
     2881 cctgagtagt gatcggcggt gctctgaatg aaagtgtagt gattggaata ccggggcaat
     2941 gcgccggcag tatatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnattttg aaaatggagt tgggctatgt ctaacttttg
     3061 atcaaaataa tatcgaatag ctctgtccct gtggataccc atatcaaata atttcgcatt
     3121 caacatcggg cttatgccag gtataggctg gaaacggttt ggatatgtga gacgggggcc
     3181 actactcgcg gtctggctct ctatagacaa gacgccgtcg tcaaacccat gatgaattcc
     3241 atgtttgact aactggctca atattctttt ttcgccggtg tccgtgccga aatctgttgg
     3301 atgcccgccc gccaatgtga gtacgcaaac aggtacttga ttcatcaagc taccccataa
     3361 atgccgggtg attgacatat caagcaattg ggatctaaac agccagtcag tgggctgatt
     3421 gacttcaaag ccaagaaaag cttctgccag gggttttaca tattgcaagc ccgtacttgc
     3481 ccccatcagc gcgagtgaag cgtagttgct cccaccaaaa gcaccctgta gcgctatatg
     3541 taattcgcaa cgttctgcaa taaaactcac atcccccaac aaaacattta aagggggaac
     3601 cccggaaaga tcactgacag taagcgcgat atcagacaat ggcgcaccat cggaatgaga
     3661 tatgatgata aaaccattat gcccaaaggt attagcgggt cttgattctg tagggtcgca
     3721 gttcacaact ccgaccccgt caatcatcgc attggcaatt tgctcgatga ctgcatgggc
     3781 gcctgcaaca aatgtttggg tggctggatg agccactaca atgtatctat ttgaatagtt
     3841 gccacttcgg atgacgttgt tgaagctatt gggatcaacc caggtgttca gatattttgc
     3901 ggtgtgtgta ttccagtatt gataagcgcc tcgcttccag tatccgttgt aaaattcgtt
     3961 tctgtttgcc ggccatgtcg tcaacgcatc cggaatgccg ttgagacgct ccatcaacgg
     4021 gtctgttcgc aatccatgca cgaaaataat atcccgacca ttgaaaggac tattcggatc
     4081 atccggcgga gagccttgcg gcggcaaatt tctacagtta aatgccaacg cgcccacccc
     4141 catgatactg tccagcatat tggcgttggg aagtgccaac tccagagagt ccataaaact
     4201 actcaaatca gatagcaagc tgtccgtact gacgccgata gaatcctgca taaaattgtc
     4261 aagcgaatca ttttctataa aatccaataa ttcgtcagga aactctcccc cgatagactc
     4321 gcaggagtcc tgacaaaact cataccaggt atcaagatca ttaggtgcgg gattgcagta
     4381 gccgtagatg tcgttcacat tccccatgag tatgtcaatc tcttcttcca aaaccgggca
     4441 agttgggtca atcagcccaa atggcggaat gacattgcca agtgagtcca ggtcagcaag
     4501 gtaacatggg atagaatcat ttacttctct aaaacagatg ttgtcaatat atccgagaga
     4561 aatcccatcc gacccaatcg ggtcgttgtt ttcaatgctg atggatatgt tgttgtagtt
     4621 tttgggagct cgccaccgat gaaacacaaa ttgggtccag tcatcacaaa cagctatact
     4681 gcctgatacg ccaatgatgg cagtgtctat accgggagcg ggatggcgcc cggatgttgg
     4741 cagcgtacca ttgaaagcga tacctcttag tttgatatac ggcacgggac acccccaaca
     4801 accagaacag gatacgcttt taacacaaac cgtcatttcg tacactttcc ctttttgtat
     4861 cggggtcgtc aactgttgat agatggccga accttgcgtc ctgttgcctt gcagttcaac
     4921 atatcctgga tcgccacaac caaaaccggt cgaaaccacc ggcgttccgt aggcggcctg
     4981 ccaattggcc atgctgccct gcaagggcat tgggccggca atggctccga cgctgaaatt
     5041 cgagttctca attatattgc cggggcatgg ttcttcacac aggacggtta catcaaacga
     5101 gcacgatgaa acatttccac agttatcagt agccgtataa gtgattgtag aaacgcctgt
     5161 gttaaaagtc aacccacttg catcgttttg gccggtggca gaagtagctc cggttacttg
     5221 gtattgaatc tgaggtgttg aacaattatc ggtagccgaa agcaagtgga ggttatttat
     5281 ggtcgcagtg caagaatctg gatttgtcga agagactacc nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnaaac accgcagcct tgcaccgtcg ccgggcttac
     5461 actcatagat tgtatctggc agttgtcgaa agagccggcg ttgatcatcg ccaccgtgac
     5521 aggcagggtg caattggcat tcaattcgta gccttgtccg ggctggcaaa gggctgtggg
     5581 cggaatattg tcggtaatat tgatggtttg ggtagcaacc gccaccgttc cgtcggcgca
     5641 ggtgatggat acgcagaaat catgcgagcc gcaggacagt atccgatcaa tcgtggcggt
     5701 ggattcaccg ctgcaccact ggtagctgaa gggctgaagc cccgaaccga cggcggtcaa
     5761 ttgcacatgg ccgcagttgt caatatagtt tacggtaata gatgcctcgc atggcaggct
     5821 gcacgcctcc gcctgctgcc aggtaaccaa actactcgcc acatccatgc ccggcagtgc
     5881 atctgtaaaa tcaatgttca ggcagcacgg gccgttgaag gttagcgcag tgtttgtcac
     5941 agttgttgtg ctggtttggg tcatgcaggt gttcatttcc agatcgctca ccgacataaa
     6001 ggcgcgctct tctgtagtgt ttcgcccgtc gcaaaaggcc atgcctgccg gtacgggcca
     6061 tgcagtaccc cctgtgtaag aggtttcatt cacttgcaga tactgcatcc attgcaaaac
     6121 aggcccattg acgccatcct gaaatttgag cactacgccc cgattaaaac cttccaccgt
     6181 tgcgctgccg ctcacaaaga tgctgctgcc cgaagtccat acgttgcgga tggccgttag
     6241 gccgggcagg ctgacttgcc aaagtgtgat gaggtcgggg tcgaatttca tcagccgggt
     6301 tgaaaaggaa gggaactgaa aaccggcggc aataaaatta ctgccggctt gcgccacatc
     6361 cgcaaaggtc atctgaatac cctcgggcga tactccgccg ataatggcgc ccgcattgtc
     6421 ggcccggaag atggcgccac tggtacccca attgccggcc agcagcaagt cgccgttggg
     6481 cnnnnnnnnn nnnnnnnnnn gcacaaactc gtcatctgtg gagccaaatg cacctgaata
     6541 tatttttttc catcctatgt tgccggcctc gctcacggtg agtagcacca cgtcgtccca
     6601 attgagcacc gcgcttggtt cttgttgaaa accaagcaca taatatggag atgctgcatt
     6661 gttgggcact ggatttcgaa ccacccgatt gaaggactct cgcccggttg cattataatt
     6721 tctcagccag ttgaacgtgc cggcaggcgt gatgacgccc atcagacttt ggctgttgac
     6781 atccaacggc agggtatgtc ctaccagaaa caggttgccg gaaggcgtca gcaccgcgtc
     6841 attccattgc gaggccacgt ctaagcggcg cgtccacagc aattgcccgg tagagttgat
     6901 gcgagatacc gtagcgcggg gttgttgccc ggccgtcgcc tctgcctgtc ccagtacata
     6961 gtaatccgag ccatgcggaa tgactttgct gaaagactcg tccagtgctg taccgtactg
     7021 gcgctgaaat tgggcttgta aaggctgaaa cgccgggaag atagagatga tggccgtgat
     7081 gcttacggcg caagtgtagc gtaagttcat agtatcgagg gttgtaagtg aaacacaaat
     7141 aatggcaaat gtaataatat aatttatgat tcaaaaaaaa taataactct aaaaacattt
     7201 ctacaaccaa agttatagaa ttttaataaa acggaaaagc cggccgcctg cccattccga
     7261 cagcccgacc ggccaaccca actagaatta tacagttgtt tggtgttact tcactttgcc
     7321 atacacccag ccgatgacgc cgccggccaa tgccccgcaa atcgtgttgc cgatcacatc
     7381 aatgggcagc cagctcaaag tgtaggcatt gaatgtgctg taggtgaaaa gcccatacca
     7441 tagtaccatc agcaaagtga cccatcctcc ggcaatgagg ccgcctttga atgtacttac
     7501 gccgaaacgg tacagtaaaa cagccagaag caggccctgt accaagctgg ccacaatcat
     7561 cgcccacatg ggcgcatctt ccatggactt cacacaggcg gaattttcag cataccattt
     7621 ttcagccata gagccgagca atcccatgaa aatagccatg gcagcaacag tgccggttac
     7681 cgcgccggcg agcgttccga aaagaatctt ttttcccatt gttttgaaaa attgtagttg
     7741 gtgatgagaa ttggtgtgaa aatcatttac aaaggttcgg gcgcgccctc gaatttttga
     7801 tgcttcccct gcagcttgcc gaagaacaaa gcgatgggaa aaatcatctt ttttacgaag
     7861 cccggaatat cgagcatatc gaattccgaa cggtagcggc ccaacagaaa ggctccgtcg
     7921 aaagcgcctg gctgcttgcc cccgttttct ttcatcgaat cgtaaatgcc ggtgaggaag
     7981 tactcgaaat tgtgtgcggg ttttacccag ccggagcaga tgagcggctc tgtgccggca
     8041 ttccagaaac ggtggggaat accggcttca aataccgctg tttcgccagg ccctactgat
     8101 aaaggcgctt gcccgagtat ctcgatgccc attgtgcctt gtactaccgt gaggctttcg
     8161 gcttgcttgt gatggacgtg catcggaggg ccgcagttgg gcgctacttc gttttctact
     8221 tccagccagt cgccggtcgg gtcttgtacc aaacgtttaa aggtaagccg ttcgccggcg
     8281 ccgttttcga tggtgtgcgg atattgaaaa tgcatggttg atgagcgttt tgagtgaatg
     8341 gaaaaaatat ttagtgggtg atttttaaac tgtttcgcca caggtaaaat cccagccata
     8401 tagcccacaa aggcgcagca aagaggtaga aattcaaccc gataccagat acaagttctg
     8461 attgaaatat ataaccaagc acatctgtca aggctgccag tccgagcaac tgtgtgaata
     8521 aagccagccc tttgtgcgcc gggcgcatga gccggcccat gccgagccac cagatggcag
     8581 ccagggtcat agaaaacagg ttccagattc cactcataac ctcgttgttg aggttggcaa
     8641 aagtttgtgc agcgactgct ttgtctgctt cgctgccgac ccgataggca tcgtacaacg
     8701 gctctgtagc gccggccagc atggcagcgc ccgtagcccc gatgaaaatg aatcctatgc
     8761 cgcacaagct gaacaattgg ctttgcaacg gcgcctgtgc cgaaagccaa cggtgcaaca
     8821 ccagcagtgc cggcgccagc ggcaggtagt aaccgaaaat atcgagtatc cagccccaac
     8881 ggagggcagt ggatggatcc ggctcgaagg caatggcttt cattggattg aaggcgatgt
     8941 cgaaatccca gttgaacgca acgaaaatga gggtg
//
LOCUS       DAORLF010000171         8923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4230_length_8923_cov_2.216396, whole genome shotgun sequence.
ACCESSION   DAORLF010000171 DAORLF010000000
VERSION     DAORLF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_8923_cov_2.216396"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1406)
                     /locus_tag="PK198_13870"
     CDS             complement(93..1406)
                     /locus_tag="PK198_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39872.1"
                     /translation="MKANSLLLLLLLPLAVFAQNNKLKSGISALKSGEYADAISRFDG
                     IIVQSSKPEERAEAHYYRAESYVKLYQEALHNRNPQQLRAYSDAYVRASEDYARAAEL
                     DKSAWQESAGKELLKLYPELVRDGLQKLEDAKKEKAPEQSSPLLRNAAQLLGVAAAQQ
                     PQQYLPADLLGQIAMEQQQYRQAEQYLRQAAVLFNQYPPENPDLLAAYIYYRLALLEY
                     SYKHKGSGPPHTDQLRTALEYLRQAKSLLESEYRRAGRMAGKLKPQELERYQRQHRSV
                     QQDFYYLELDLYLQLPELHHEAIGKFKEALRKEPDSYPLTMGYARLLEKNDLQQALAM
                     YQKAAMLQPESFAANYHIGIIYVNEAARFEKEAKQTANIDRYQQLNALSREYLAKARP
                     YLQTAYRSDPDNLDLVNALLQVTLNLHLPDDYRLYKRKQMELGEK"
     gene            1557..2234
                     /locus_tag="PK198_13875"
     CDS             1557..2234
                     /locus_tag="PK198_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39873.1"
                     /translation="MRYICETFFPQPKSQNTIYRLFLMNATEIVVKYCSGTSREKHEA
                     VQFMLEWLIPRATAYMYKNNGKNQDEMIQEVAWESLTGRNKLLERLCSQDMGSIEAYY
                     LTIVRNEWSRLQQQKQSIVEVDAITYSTAFSSSAELIELLSKIKRLSTIYQAPLLISA
                     MGFSYLDIAVEYDEIKLRVSDMLRNYEQNPEDFELEGDIFDISNDTFRQRLSKGRRRL
                     KALYHRN"
     gene            2244..3299
                     /locus_tag="PK198_13880"
     CDS             2244..3299
                     /locus_tag="PK198_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39874.1"
                     /translation="MNDIHFDEVFSYLNGEMSSEQAAAFEQRLASEPLLQEELNMQKS
                     TMDNDSGRFEAILNQAHRTLQSPDRLFQRLRVQSDQIVLQTLRDLENDIQAADVPAVS
                     ATLEQAASPPAEKKLKRLVINRTLFNALRIAAVVTPLVVAVTAGWYYWGMKEAAPVAH
                     ESPASPRENGMESLPENALIALVNTAIDNLPGAKEKLPTTGENLHGKSPEYHDTKTAT
                     NGGLLTPSDRSPKDVNTNSKAEIPTNGELLTETEHCSLEAGITRLRKGQFVSAQQIFE
                     ELLNKKCLNEANAAYAKYSLALSLLAQQDTDQHAWAIRLLNEAKPDLPDKEQMTLSAL
                     IAKLPSAKSSGRIARPE"
     gene            3415..3942
                     /locus_tag="PK198_13885"
     CDS             3415..3942
                     /locus_tag="PK198_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRF39875.1"
                     /translation="MTLIIDPLEQEQEQPQKKSKPDSAKPLVGFLFSYSKTAEGEYWP
                     LFEGKNTVGSHKSCHIALYEQSVSNPHANIFAIRKKGEERLEFRIQDAGSEISTIVDG
                     EQLMVGDVASLKDRSVIQIGKYNLFCVLIDYVADQMAPNPDFSPVGKADKDFSTLKEI
                     AAQKESGKNPNFTTT"
     gene            3963..5759
                     /locus_tag="PK198_13890"
     CDS             3963..5759
                     /locus_tag="PK198_13890"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRF39876.1"
                     /translation="MHTITGSRGSYRFDFKDRLNDERGAMSAALFTGVSLDDRPRPVA
                     IKLLSERTVNNPVNLARTKAIVEATVRHENVVELIDFCEEEDIHGDKFYFVVMEMLEG
                     ENVADRFNRFFKDHQKPYPEDTVLHLSRQVLAGLKAIHDKGIVHRDVDPSNWMVCTPD
                     EGLKIIDLDIAYLPRATYGTNRGTRLGKLSYMPPQQIDGLETPKPNWDLYALGISMYQ
                     LLTGELPYSVGEEAPMIHAIRNKPLPKHEHLSPRMFSVLQKATAKLSENRFQSADEFL
                     AALEPPKKRPSPVAVFRAALQQVGTIAWPLAALKTVFTWAGSALVIALVVWGAMKVLQ
                     RCEFRTETPAPPPVAVIEVINNACEAPCAVTLRSVSENTDEQTAFSWTDENGMVLSTE
                     TVYRYEETGMAQITLTVKNEDGQSDSATRQVEIRRASPPVADFTLNTSGCEAPCTVSA
                     SANATGEPSGTVYKWTGGVTTQEGRETQFSFKQPGKYRITLTVTRADGTSASESREVE
                     VTKKVVEPPPTLPVNPTRVEPAPCQSPVLEQAVINRINQIGTYIKAVPKNSGNKKGLQ
                     IHYRDKQNIERTYDAIDEDNDCKIKTHEAPDF"
     gene            5792..6805
                     /locus_tag="PK198_13895"
     CDS             5792..6805
                     /locus_tag="PK198_13895"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRF39877.1"
                     /translation="MKIQSMGQASLQGGRDQNQDSILTETLPNGWVLAAVCDGMGGLD
                     DGKMASVLALNRISAHCQANYQFSSPAQLMRDAIEKAYDAILQQSIDNEIRLGTTVAL
                     LLLTPETACVAHLGDSRVYQIRKGRILFRTTDHSKAFQHVQAGVMTEEEARIHPDSNK
                     LTKGLIPDRFDQPDIMELSYRKGDLFFLCSDGVWNLRSEQELIRLITSEPNAQSMVPW
                     LLHSLDNAANNDGGGHDNLSAIALHTSDSSRLKTVSRNSYLLWLGILLAGFTLSLGGV
                     RVAFPELFTSKDNSPVQQTPSSPTADESLQDSLEKAMSDVRILRDSLEYYRSQNTQPK
                     YAR"
     assembly_gap    7147..7362
                     /estimated_length=216
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7363..>7650
                     /locus_tag="PK198_13900"
     CDS             <7363..>7650
                     /locus_tag="PK198_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRF39878.1"
                     /translation="THAMIRVDKNGVVTLYDKSVNGTTLNGAPVQRFTEIPVKHGKDR
                     IIFAGRYELNWSQIPKPTSHRKLLVPVAGVLAVLAIVFVLWKNFNSDKTAPL"
     assembly_gap    7651..7723
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7724..8585
                     /locus_tag="PK198_13905"
     CDS             <7724..8585
                     /locus_tag="PK198_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRF39879.1"
                     /translation="SVLVIRYSIAKLLFGNDVIYFGSDPQQQPSLSKENLKPFTSTGT
                     AFGVREDGYFTTNYHVVASYAESEKALADELLLKYRKAMEAIIKYANSIGYKIKYEDI
                     TYETQKIVVVKDGNRVYSRTFTNYENLPAGQFYEVTIHLEDQLNDLAIIRLADSDLNK
                     QFSYLPFDCIGNDDDIKPNTQVRYIGYPANKIINFEGGEIYYDVSKYRSSGQINKLED
                     NGRDFMADYTNAGGASGSPVYDLQTGKLIGINRAMRPDAPNQQSGTTSFAIKVGHLRK
                     LMEQLPPVNN"
     gene            8654..>8923
                     /locus_tag="PK198_13910"
     CDS             8654..>8923
                     /locus_tag="PK198_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39880.1"
                     /translation="MRIYIVFFIIAIPQFIFAQEYQPVACPLGQEQTILNLPKPGAGK
                     LSNAENDIRAFVRDSLPSVVNRKFISHSQRYFSIKKVPDATIYRID"
BASE COUNT         2417 a         2083 c         2082 g         2052 t
ORIGIN      
        1 attttagggg taagtatgaa tggctttttc agaaaaacgg tgcaaaggta agcgaacagt
       61 tttgcaccgc aaagcaaaag attattttgt tgtcattttt ctcccaactc catttgcttg
      121 cgcttgtaga gccggtagtc gtcgggaagg tgcagattga gggtcacctg aagcagtgca
      181 ttgaccaaat cgaggttgtc ggggtcgctt ctataggcag tttgaagata tgggcgggct
      241 ttcgccaaat attctcttga taacgcgttc aactgctgat agcggtctat gttggccgtt
      301 tgtttcgctt ctttttcaaa acgggcagct tcattcacat aaatgatgcc gatgtgataa
      361 ttggctgcaa aagattcggg ctgcaacata gcggcctttt gatacatggc cagggcttgt
      421 tgcaaatcat ttttttccag cagccgggca taccccatcg tcagagggta gctgtcgggt
      481 tccttgcgca gcgcctcttt gaacttcccg atggcctcgt gatgcagctc cggcaattgc
      541 aggtacaaat ccaactctaa ataataaaaa tcctgctgaa cagaccggtg ctgacgctgg
      601 tagcgctcca actcctgcgg cttcagcttg ccggccatac gccccgcacg ccggtactca
      661 gattcgagca ggctcttggc ctggcgaagg tattccaatg ctgtgcgcaa ctggtcggta
      721 tgtggcggcc ccgagccttt gtgcttatag ctgtactcca gcaaggccaa acgatagtat
      781 atgtaggccg ccaacagatc agggttttcc ggcggatact ggttgaacaa cacagcagcc
      841 tgccggaggt attgctcggc ctggcgatac tgctgctgct ccatagcaat ctgtcccagc
      901 aggtcggcgg gcaggtactg ttgaggctgt tgggcggcgg ctacccccag cagttgcgcc
      961 gcattccgca aaagcggcga ggactgttct ggagcttttt cttttttggc atcttcgagt
     1021 ttttgcaggc cgtcgcgcac caattcggga tacaatttga gcaattcctt ccccgcactt
     1081 tcctgccagg ccgatttgtc caactcggcg gcgcgggcat aatcttcgct ggcacgcaca
     1141 taagcgtcgg agtacgcgcg caattgctgg gggttccggt tgtgcaaggc ctcctgatag
     1201 agtttcacat agctttcggc gcggtaatag tgcgcttcgg cgcgttcttc gggcttggaa
     1261 ctttgcacaa taatgccgtc aaaccggctg atggcatctg catattcgcc gcttttgagc
     1321 gcgctgatac cgcttttgag cttattgttc tgggcaaaaa ctgcgagcgg caacagcaat
     1381 agcaacagaa gcgaatttgc tttcatatta tttttcggca aatgtaagac ttgatacccg
     1441 atggcggtat gaaaaagtaa ttcgttcctg ataagaatcg tccataaagc caatccttgc
     1501 cttgtatgcg gcattcgtat ttttaaaaat gtatcaagtt tgaaaaaata cgatgagtga
     1561 gatatatttg cgaaacattt tttccacagc caaaatcaca aaacaccatc taccgactct
     1621 tccttatgaa tgccacagaa atcgttgtca agtactgctc aggtacaagc agagaaaagc
     1681 atgaggcagt gcaatttatg cttgagtggc tcatacccag agcaacggcc tatatgtaca
     1741 agaacaatgg aaaaaaccaa gatgaaatga tccaagaggt cgcatgggaa agcttgaccg
     1801 gacgcaacaa actgctggaa cggctttgtt ctcaagatat gggtagcatt gaggcatact
     1861 atcttaccat agtaagaaat gaatggagcc gcctccaaca acaaaagcaa tcaattgtag
     1921 aggttgatgc gatcacttat agtacagctt ttagttccag tgcagaactg attgagttat
     1981 tgtcaaagat aaaaagactg agtacaattt atcaggcgcc cttgctcatc agcgctatgg
     2041 gattcagcta ccttgacatt gcggtggaat atgatgaaat caagttgcgc gtatccgaca
     2101 tgttgagaaa ttatgagcaa aacccggagg actttgagtt agaaggggat attttcgata
     2161 tttccaacga cacttttcgg cagaggttgt caaagggacg tcgcagactt aaagcattgt
     2221 atcacagaaa ctaatcggca atcatgaatg atattcattt tgacgaagtt ttttcatatc
     2281 tgaatggcga aatgtcatca gaacaggctg ctgcatttga gcagcgtctt gcttccgagc
     2341 ctctgttaca ggaagaactg aatatgcaaa aaagcacaat ggataatgac tccgggcgct
     2401 tcgaggccat actcaatcag gcgcaccgta cgctccagtc acctgatcgc ctgtttcagc
     2461 gtcttagagt gcaatccgat cagatcgtcc ttcaaacact acgagacctc gaaaacgaca
     2521 tacaagcagc cgatgtcccg gctgtatctg ccacgctgga gcaagccgca tccccccccg
     2581 ccgaaaagaa attaaaaagg ctggtcatca acagaacact ttttaatgcg ctgcgcattg
     2641 cggcggtagt gacgccattg gtggttgccg ttactgccgg ctggtattat tggggaatga
     2701 aagaggccgc tccggttgca catgaatctc ccgcctcgcc tcgtgaaaat ggaatggaaa
     2761 gccttcctga aaacgcgctg attgcacttg ttaatacagc catagacaac ctgccgggcg
     2821 ctaaggaaaa gctaccgacc acaggcgaga acctgcatgg caagtcgcct gaataccacg
     2881 acacgaaaac agcaacgaac ggcgggctgc tcaccccttc agatcgttct cctaaagacg
     2941 tcaatacaaa ctcaaaggca gaaataccca ctaatgggga gcttttaaca gaaacagagc
     3001 attgttctct tgaggcggga ataacccggc ttcgtaaagg tcaatttgta tcggctcaac
     3061 aaatttttga ggaattgttg aataaaaaat gcctgaatga ggccaatgcc gcctatgcta
     3121 aatacagttt ggcactgtcc cttttggctc agcaagatac cgatcaacac gcttgggcca
     3181 tcagactgct gaacgaagca aaacccgact tgcccgataa ggagcagatg accctgtcgg
     3241 ccctgattgc aaagttgccc tcagcgaaat cgtcaggcag gatagcaagg cctgaataaa
     3301 aaacagaaaa aaaacacaag aggcggtcac aaaatcccga tgagtcgctc ttcaagtaaa
     3361 acagcattta aatcgtactt caacaatttt gttcacctat ttaaaacttc catcatgacg
     3421 ctgattattg accctctcga acaggaacag gagcagcccc aaaagaagag caaacctgat
     3481 tccgctaaac cactagttgg ttttttattc tcttattcaa aaactgcgga aggcgaatat
     3541 tggccgctgt ttgagggtaa aaataccgtg ggcagccaca aaagctgcca catagcgctg
     3601 tacgaacaaa gcgtatccaa tccgcacgcc aatatttttg cgattcgcaa aaaaggagaa
     3661 gagcgtttgg agttcaggat tcaagatgcc ggctctgaaa tttcgactat tgtggacggg
     3721 gaacaattga tggtggggga cgtagcaagt ctgaaagatc gcagcgtcat tcagattggt
     3781 aagtacaacc ttttctgtgt ccttattgat tatgtggcgg atcaaatggc gcccaatcct
     3841 gacttctcgc ctgttggcaa agcagacaaa gattttagta ccctcaagga gattgccgcc
     3901 caaaaagaaa gcggcaagaa ccctaatttc actactacat gacctcatag actccaagcg
     3961 ccatgcacac tataaccgga agccggggga gttatcgctt cgacttcaag gatcggctca
     4021 atgacgaacg aggcgccatg tcggcagcat tgttcacagg ggtatcgctg gatgaccggc
     4081 caagacctgt agccattaaa ttgctcagtg agcgaaccgt aaataatccg gtcaatctgg
     4141 cgcgtacaaa ggccattgta gaagccactg tgaggcatga aaatgtggtc gaattaattg
     4201 atttttgcga agaagaggac atacatggag ataagtttta cttcgtcgtt atggagatgt
     4261 tggaagggga aaatgtggcc gacagattca acaggttctt taaagatcat caaaagccat
     4321 atccggaaga caccgtacta catttatccc gacaagtttt ggccggtctg aaggccattc
     4381 atgacaaggg aattgtacac agagatgtgg acccctcaaa ctggatggtt tgcacgcctg
     4441 acgaaggtct caaaatcatt gacttagaca ttgcctattt gccaagagcg acgtatggca
     4501 ccaacagggg aacgcgattg ggcaagctaa gttacatgcc gcctcaacaa attgatggat
     4561 tggagacgcc aaaaccaaac tgggacttgt atgctttagg tatctccatg tatcagctcc
     4621 ttacgggcga gttgccttac agtgtaggcg aggaagcgcc catgatacac gccattcgga
     4681 acaagccgct acccaagcac gagcacttgt cgcctcgtat gttcagcgtg ttgcaaaaag
     4741 ccaccgccaa gctgtcggaa aaccgctttc aatcggcaga tgagttcttg gcggcattgg
     4801 agccgcctaa aaaaagacct tcgcccgttg cagtgttccg agctgcgtta caacaagtgg
     4861 gaacaattgc ctggcccctt gccgcattaa aaacagtatt cacctgggca gggagcgctt
     4921 tggtcattgc gcttgtcgtc tggggtgcca tgaaagtgct tcaacgatgt gagttccgta
     4981 ccgaaactcc tgcgccgccg ccggtagccg ttatagaggt aataaataat gcctgtgaag
     5041 ctccttgcgc ggtaacgctg cgcagtgtat ccgaaaacac agacgaacaa acggcattta
     5101 gctggaccga cgaaaacggc atggtactct ccaccgaaac agtgtatcgc tacgaagaga
     5161 ccggaatggc tcaaattacc ctgaccgtca aaaatgaaga tggccagtcg gatagtgcga
     5221 caaggcaagt agagatacgg cgggcaagtc cgcccgtagc ggattttacg ctcaacacat
     5281 ctggatgtga agcgccctgc accgtaagcg cttcggcaaa tgccacaggg gagccatccg
     5341 gaactgttta caaatggacc ggtggagtta ccacacagga ggggagagag actcagttct
     5401 cgtttaaaca acccggcaag taccgcatta cgctcaccgt tacgcgagcc gacggaacat
     5461 ctgcctccga gtcgcgcgag gtagaagtta caaaaaaagt agtcgaacct ccccccaccc
     5521 ttccggtaaa cccaacccga gtagagccgg cgccatgcca atcgcctgtg ttagaacagg
     5581 cagtgataaa tcgaatcaat caaattggta catatataaa ggcagtgccg aaaaatagcg
     5641 gaaacaaaaa aggcttgcag atacactacc gtgacaaaca aaatattgag aggacttatg
     5701 atgccattga tgaagataac gattgcaaaa taaaaactca tgaggctccg gatttttaat
     5761 catttcctct tttctcaaag acaaaactta tatgaaaata cagtctatgg gtcaggctag
     5821 cctgcaaggc ggcagagacc aaaatcagga cagcatcctc actgagacgc ttcccaacgg
     5881 atgggtgctc gctgccgtgt gcgatggaat gggcggcctc gatgatggca aaatggcctc
     5941 cgtattggcg ctgaatcgga tttcggccca ttgccaggcc aactaccagt tcagcagccc
     6001 ggcgcaactg atgcgcgatg ccattgaaaa ggcttatgac gccattttgc agcagtccat
     6061 tgacaatgaa atcaggttgg gcaccaccgt tgccttgttg ttgttgactc ccgaaacagc
     6121 ctgtgttgcc catctcggcg acagccgggt ttatcaaatc agaaaagggc gtattctgtt
     6181 tcgcaccacc gaccactcta aggcattcca gcatgtgcaa gccggagtga tgaccgaaga
     6241 ggaggcgaga atacacccag actccaacaa gctcaccaaa ggacttattc ccgaccgttt
     6301 cgatcagccc gatatcatgg aattgagcta tcgcaagggc gatctgtttt tcttgtgttc
     6361 agacggcgtt tggaatttgc gcagcgaaca ggagttgatt cgtttgatca cctctgaacc
     6421 caatgcgcaa agtatggtgc cctggctgct ccattcctta gataacgcgg ccaataatga
     6481 cggaggcggc cacgacaacc tctcggccat tgccctgcat acatcagact cctctcgctt
     6541 aaagaccgtt tctcgcaaca gctacttatt gtggctgggt attttgctgg ccggttttac
     6601 tcttagcctg gggggcgttc gcgtggcttt tcccgaattg tttaccagta aagacaattc
     6661 acctgtacag caaacaccct ccagccccac tgcggatgaa tcgcttcagg atagtctgga
     6721 aaaagccatg tcggatgtga gaatattgag agactcgctg gaatattacc ggagtcaaaa
     6781 tacacagcct aagtacgccc gatgagcctg tgttaaaaaa atcagaaaaa aaaccgcgtt
     6841 tgcccttcac aaaatcgcag gctgaagact ctacagagca taagaagccg gttgttccac
     6901 agaacgatca ggataaggcg ccggatgtgc cgctgaagct cccggaaccg gattcgacac
     6961 agcaaactca ttaacaattt caatacaaaa cgaaaaaaat ctcctcttat gaagaccatc
     7021 attaccatcg gcagcggccg taccactccc agccacatcg ttcttcaggg cgatcacatc
     7081 agtgacaaac acggggaatt agtccttttt gaaggcggcc gcatcaccta cgaagactac
     7141 agcagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnactcatgc catgatccgg
     7381 gttgacaaaa acggcgtcgt aactctttat gacaaatccg tgaacggcac tacgctcaat
     7441 ggcgccccgg tgcagcgttt taccgaaatc cctgtgaagc acggtaaaga ccgcatcatc
     7501 tttgccggtc gctacgagtt gaactggagt cagataccca aaccaacgag ccatcgaaaa
     7561 ctattggtac ctgtagccgg cgtgctggcg gtgctggcaa ttgtgtttgt tctatggaaa
     7621 aattttaact cggataaaac agccccactt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttctgtt ctggtgatta
     7741 ggtatagcat agccaagctt ttatttggaa acgacgtgat ttattttggc agtgaccccc
     7801 aacaacaacc ttcgttatcc aaggaaaact taaaaccttt caccagtaca ggcacagctt
     7861 ttggtgtaag ggaagatggg tacttcacga ccaattacca tgtggttgcc agttatgctg
     7921 aatcggaaaa agcattggca gatgaacttt tgctgaagta tcgcaaggca atggaagcga
     7981 tcatcaaata cgcaaattca attgggtaca aaatcaaata tgaggatatc acctatgaga
     8041 ctcagaagat agttgttgtg aaggacggca atcgggtata ttccagaaca tttacgaatt
     8101 acgaaaactt gcccgccggc caattttacg aagttaccat tcatcttgaa gatcagttaa
     8161 atgacttagc tataataagg ctagcggact ctgatctgaa taagcagttt agctacttac
     8221 catttgactg catcggcaat gatgacgata ttaagcctaa cacacaagta cgatacatcg
     8281 gctatccagc gaacaaaatc ataaattttg agggtggcga aatttattac gatgtgagta
     8341 agtaccgcag tagtggccag atcaataagt tagaagataa cggtcgtgat tttatggcag
     8401 attatacgaa tgcgggcggg gcgagcggat cccctgttta cgatttacaa accggtaaat
     8461 tgataggcat caatcgggcg atgcgccctg atgctcccaa tcaacagtct ggcaccacct
     8521 cctttgccat caaagtcggg cacttgagga agctcatgga gcaattgccc cctgtcaata
     8581 actaaggcat agatatcaaa gttcgggagg tcgtcaatgg cctcccgcct atatcccttc
     8641 taaagttgca agcatgcgca tttacattgt attttttatc atcgcaattc cgcagttcat
     8701 ttttgctcaa gaataccagc cggtggcttg ccctttggga caagaacaaa ctattttaaa
     8761 tctacccaag ccaggcgcag gcaaattgag taatgctgaa aacgacatac gtgcttttgt
     8821 gagggattct cttccatcag tagtcaaccg caagtttatt tctcactcac aaaggtattt
     8881 cagcatcaaa aaagtgccgg acgcaacaat ctaccggatt gat
//
LOCUS       DAORLF010000172         8920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4232_length_8920_cov_2.034292, whole genome shotgun sequence.
ACCESSION   DAORLF010000172 DAORLF010000000
VERSION     DAORLF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4232_length_8920_cov_2.034292"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2561
                     /locus_tag="PK198_13915"
     CDS             <1..2561
                     /locus_tag="PK198_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39881.1"
                     /translation="QFMEKQLFTMLIFLLGAWMGLTAQPVIQGKLTDASTKEPLVGAS
                     VLIKGTITGVSTSLEGTFEIPYSGKFPVTLQFSYIGYEKQELLIESNRPLNISLRPAG
                     SNLMEVVVTSRRRKEEVQEIPIPITVLGASQIDNSGSFNVNRVKELAPSVQLYSSNPR
                     NTTINIRGLGSTFGLTNDGIDPGVGFYVDGVYYARPAATTIDFIDIEQIEVLRGPQGT
                     LFGKNTTAGTFNITTRRPSFDVGANFELSYGNYGFIQAKSSVTGPVLKEKIAARLSFS
                     GTYRDGVIYNVATQKNVNTLNNLGVRGQLLYKPGKNVDILLAIDHTRQRPDGYAQVFA
                     GVAPTRRAAYRQFESIIADLEYELPSRNPYDRIIDHDTPWRSGQDMGGISLNADVKLG
                     KGQLTATSAWRYWNWDPSNDRDFTGLQGLRLSQAPSIHQQFSQEIRYAGDFGKKLSGV
                     FGVFAFYQTLDADGAHTEEAGRDQWRFSQSSSSPLWQTEGLLEGYGIKTYPSFRNFSG
                     AVFGQIDWKVLPKLSILPGIRLNYDQKEVNFRREIYGGLQTADPALIALQRSVYSNQA
                     FDAEVDKANLSGQITAAFEAHKKARIFATYALGFKPVGLNLGGLPSAGGQPLTELAVI
                     RPEQVQHYELGLKTQPVNRSILNLTLYNTEIQDYQTLVQTADLAVNRGYLSNAERVRV
                     FGVELDASYRLPRYLSLSGALSYTNGKYVSFTNAPPPLEETGGPTFKDISGGDLPGIS
                     KWSFSLNAEATVKGNLIGQKGEYFMGADTYYRSSFSSSPSPSAYLVVDGYALLNARIG
                     FRAFEGFSLFVWSRNLLNQNYFEQLLPGAGNAGHYAAVLGDPRTFGVTLRYNLL"
     gene            2625..>3591
                     /locus_tag="PK198_13920"
     CDS             2625..>3591
                     /locus_tag="PK198_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39882.1"
                     /translation="MLLCGICHWTFSQSSDSSGLPKFWIDIEIRPRAEFRDNYIFSAN
                     DTLLPDFHISQRNRITLNYIRGKTTMLAAFQEIHLWAKSGTPSSVANIGAYELYVESR
                     LNDNLSFRIGRQGVSLDNGRIFSDAPWAQQGRSHEGLRMMYKNKGFSSDLFALAARDY
                     SRHFDPRYSPVSSHQYEALLIHHFKYRPNALMTITGLNSVDFFKNSSLSERYARFTTG
                     GRIELEKGDFYGTLNGYYQFGKNAQLKQIHAFYLQPECRITAGKSVLRLGAEILSGDH
                     PQINNRLTGSFDVLYGVAWKFMGNMNLFTRFPEDVSGKGLINPYLF"
     assembly_gap    3592..3601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3602..3887
                     /locus_tag="PK198_13925"
     CDS             <3602..3887
                     /locus_tag="PK198_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39883.1"
                     /translation="NFNPRLSVRSDIHLFFTQYPRLSQSSDNYLGFENDLSAKYKPSK
                     NLEINYGFSYLIPPKRAGLLPKVRDVSKPALWSYLMVSYRFEVKTGKSTS"
     gene            complement(3909..4679)
                     /locus_tag="PK198_13930"
     CDS             complement(3909..4679)
                     /locus_tag="PK198_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRF39884.1"
                     /translation="MFFILSKVLAFTIKPVVWLLALLAYALLTKHPRRSRRAIIVALL
                     LLLLMSNRWISNQVFRVWEPETITADQITEPYDIGIVLGGYSNFYIEPGHDRHNLSER
                     GNRLTNALELYFSGKIKKILLSGGSGRLLQDGPSEAEMVFALLERWGVPESDIILEPD
                     SKNTWENAEFTNNIIQTRHPGASCLLITSAWHLPRAQACFQKAGLNATPFAVDYISEA
                     NRWDFDLLLMPDKLGLYKWELLIKEWIGLLAYQMQGYN"
     gene            complement(4844..5425)
                     /locus_tag="PK198_13935"
     CDS             complement(4844..5425)
                     /locus_tag="PK198_13935"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRF39885.1"
                     /translation="MKSNQWYALAFAGMCVLYLAIPVLMIRQQEGILKEGTVFRFALQ
                     PIDPPDAFRGRYLELNFNMPAFPPPGKLVEYNQKVFLTVTKDSAGMGQFEALHADPPH
                     DTPDYVAARVAGTSSHEIHCFVPDEMRRFYLNEDLAPLADKWYAELLNRDDRRQVPVT
                     VDLRVLKGKALIEQLYFEGVPVAEYIRGKEREE"
     gene            complement(5442..5834)
                     /locus_tag="PK198_13940"
     CDS             complement(5442..5834)
                     /locus_tag="PK198_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39886.1"
                     /translation="MLIHYTHRNGLSVDFMVPKTRKGRQTRRYDHIGVWHYLLEFDPE
                     GRLSFDAATSIDFESMARHLLAVDDAARANGLRIRKVIFKLNLKDDLFKTPSGRQLQR
                     RNIYFARQLPKRVDDVHDDHYHVDFEIR"
     gene            complement(6234..>7024)
                     /locus_tag="PK198_13945"
     CDS             complement(6234..>7024)
                     /locus_tag="PK198_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39887.1"
                     /translation="DTPEAMIENKYGKNIVLYPFPNRLKNGLYHWQGKTYQFAINNAA
                     TGNAIHGFGRDRPMQIARTDITAEGASITCVRHEPGDEAAYPFIFSFFITFSLHTDGS
                     FEMDMKIRNHSDTPIPAGIGWHPYFTLADSVDEMSLQLPPCRKVEIDETMIPTGQLLP
                     EARFEQLQTIGEQVIDNCFALSGEESVAEVLLSGPRGRLRYLQEAGPVKFRFVQVFTP
                     PHRRSISVEPMSCNIDAFNNGMGLWNLEQGEEAGAAVRLRWESA"
     assembly_gap    7025..7101
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7309..7405
                     /gene="ffs"
                     /locus_tag="PK198_13950"
     ncRNA           7309..7405
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK198_13950"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7470..>8003
                     /locus_tag="PK198_13955"
     CDS             7470..>8003
                     /locus_tag="PK198_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRF39888.1"
                     /translation="MQFFIDTASLDLIKEARDLGILDGVTTNPSLMAKEGITGEANIL
                     KHYEKICKLVDGDVSAEVISTDFDGITAEGKRLSLIAPNIVVKVPMIKEGVKAMSYFR
                     DNGIRTNCTLVFSAGQAILAAKAGATYVSPFIGRIDDVSWDGMELIRQIAEIYGIQGF
                     ETRILAASIRNGKHIVDA"
     assembly_gap    8004..8027
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8249..>8635)
                     /locus_tag="PK198_13960"
     CDS             complement(8249..>8635)
                     /locus_tag="PK198_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRF39889.1"
                     /translation="FYKLISGSPRSRPLMYAGWALLAAYLGWFLYQLLPVAGALQIPV
                     AVYGLVIGSMLALAMAGYSYWSKRAALWIIGGAVLFVASDSLLAWDKFYAELPFARLL
                     VMSTYLSAQYAITVGVLKQTGVKNEG"
     assembly_gap    8636..8701
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8702..8902)
                     /locus_tag="PK198_13965"
     CDS             complement(<8702..8902)
                     /locus_tag="PK198_13965"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39890.1"
                     /translation="MSPSNQLRIYLIAAVLYLAGIYFLPDSARIIFKALLVPMLLNAV
                     WNTRAFSGKNLLMAALFFSFAGD"
BASE COUNT         2117 a         2273 c         2235 g         2118 t
ORIGIN      
        1 cgcaattcat ggaaaaacaa ctatttacaa tgctcatttt tttgttgggc gcctggatgg
       61 gcctgactgc acagcccgtg attcagggga aattgacgga cgcatctact aaagagccgc
      121 ttgttggagc ctccgtactc attaaaggaa ccattaccgg cgtctccaca agtttagaag
      181 gcactttcga gataccctat tccggtaaat ttccggtaac gctccagttc tcatacatcg
      241 ggtatgaaaa gcaggaactg ctgatcgaaa gcaatcgccc gctcaatatc agcttgcggc
      301 cggccggctc taacttgatg gaagtcgttg ttacctccag acgccggaaa gaagaagtgc
      361 aggagatacc cattcccatc acggttttgg gtgcgtcgca aatagataac tccggttcct
      421 ttaatgtgaa ccgcgtcaaa gagctggcgc cgtctgttca attatactct tccaacccac
      481 ggaatacgac catcaacatt cggggtttag gttctacttt cgggcttacc aacgacggca
      541 ttgaccccgg agtgggcttt tatgtggacg gcgtttatta cgcccgtccc gcagccacca
      601 ccattgattt tattgatata gagcagatcg aggtattgcg cgggccacag ggaacgcttt
      661 tcggtaaaaa cacaacagcc ggtactttca acattactac gcgaaggccc tctttcgatg
      721 tgggcgccaa ttttgaactc agctacggca attatggctt cattcaggcc aaaagctcgg
      781 tgaccggacc ggtattgaaa gagaaaattg ccgccagatt gtctttcagc ggcacatacc
      841 gcgacggcgt catttacaat gtagccactc aaaagaacgt aaacacactc aacaacttag
      901 gagtaagagg gcagttgctt tacaagccgg gcaaaaacgt ggatatttta ctggccattg
      961 accatacccg ccaacgcccc gacggctatg cgcaggtatt tgcaggtgta gcgcccaccc
     1021 ggcgtgccgc ctaccgccaa tttgaaagca ttatcgccga tctcgaatat gagttgccca
     1081 gccgcaaccc gtatgaccgg atcattgacc atgatacgcc ctggagatcg gggcaagaca
     1141 tgggcggcat ctccctcaac gcagatgtaa aattgggcaa gggccaactg accgccactt
     1201 ctgcatggcg ctactggaac tgggacccgt ccaacgaccg agattttacg ggcttgcagg
     1261 ggttgaggtt atcgcaggca ccctccatac accagcagtt ttctcaggaa atcaggtatg
     1321 ccggcgactt tgggaaaaag ctgagcggag tgttcggcgt atttgctttt taccaaactc
     1381 tggatgccga cggcgcgcac acggaagaag cgggcaggga tcagtggaga ttttctcaaa
     1441 gttcttccag cccactatgg caaacagagg gcctgctgga aggctacggc atcaaaacct
     1501 atccttcgtt cagaaatttc agtggcgcgg tatttggtca aattgactgg aaagtgcttc
     1561 ccaaactaag catcctgccg ggaatacgac tcaattacga tcagaaagaa gtgaacttcc
     1621 gcagggaaat ttatggcgga ctgcaaaccg ccgaccctgc actgattgcc ctgcaacgat
     1681 ctgtttacag caatcaggct tttgatgcgg aagttgacaa agccaatctc tctggccaga
     1741 ttaccgccgc gtttgaggcg cacaaaaaag ccaggatttt tgcaacctat gcacttggtt
     1801 tcaaaccggt cggactgaac ctcggcgggt tgccaagcgc cggcgggcag ccgctgacag
     1861 aactggctgt aatacgcccc gaacaagtac aacactatga actggggctg aaaacgcagc
     1921 ctgtaaaccg ttccatactg aacctgacgc tgtacaacac agaaatacag gattatcaga
     1981 cattggtgca aactgccgac ctcgccgtga acagaggtta cttgtccaat gccgaacgcg
     2041 tgcgggtttt cggtgtggaa cttgacgcga gctaccgcct gccgcggtac ctctctttgt
     2101 cgggagcgct gagctatacc aacggcaaat atgtgtcgtt cacgaatgcg ccgccgccac
     2161 ttgaagaaac gggcgggcca acgttcaaag atatttcagg cggcgatctg cctggtattt
     2221 ccaaatggtc cttctctctg aatgcggagg cgaccgtaaa aggcaatctc attggtcaga
     2281 aaggcgagta ttttatggga gcggatacct attaccgctc atcgttttca tccagcccgt
     2341 cgccgtctgc gtatttggtg gtggacggct atgcgctgct gaatgcccgg attgggtttc
     2401 gcgcttttga aggattttct ttgtttgtct ggagccgaaa tttgctcaat caaaactact
     2461 ttgagcaatt gttgccggga gcgggcaatg cagggcatta tgcagcggta ctcggagacc
     2521 ccagaacctt tggggtgaca ctgagataca acctgctttg attcgcacca gcttgatgtc
     2581 caatgactaa atgttgcggc agcaatagga taatgtgtgc agttgtgttg ctgtgcggca
     2641 tttgtcattg gactttttcc caaagttccg attcttcggg attacctaaa ttttggatag
     2701 atatagaaat aagaccgcgt gcggaattcc gggacaacta catcttttct gcgaacgata
     2761 cgctccttcc cgattttcac atcagccaaa gaaatcgaat taccctcaac tatattcgag
     2821 gaaaaacaac gatgctcgct gcctttcaag aaattcacct gtgggcaaaa tcaggaacac
     2881 cttcttctgt tgcaaatatt ggagcgtatg agttgtacgt tgaatctcgg ttgaatgata
     2941 acctgtcgtt cagaatcggg cgtcagggcg tgtctttaga caacggccgt attttttcag
     3001 atgcgccctg ggcacaacag ggcagatcgc acgagggctt gcgaatgatg tacaaaaaca
     3061 aaggcttctc gagtgatctt tttgctctgg cagcccgcga ttattcccgg catttcgatc
     3121 cccggtattc tccggtatct tcgcatcaat acgaggcttt gctgattcat catttcaagt
     3181 atcggcccaa cgctttaatg accattaccg gattgaattc agtagatttt tttaaaaatt
     3241 cctcgctcag tgaaaggtat gcacgattca ccaccggcgg cagaatagag ttggaaaaag
     3301 gagatttcta cggcaccttg aatgggtact accagtttgg aaaaaatgct caattgaaac
     3361 aaattcatgc cttctatttg caaccggaat gcagaatcac tgccggcaag tccgtattgc
     3421 gtctgggcgc ggaaatcctg agcggcgatc atccgcaaat aaacaatcgc ttgacaggaa
     3481 gctttgacgt actctatggt gttgcatgga agtttatggg gaatatgaat ttattcaccc
     3541 gctttcccga agatgtaagc ggaaaaggat taatcaaccc ttacctcttt gnnnnnnnnn
     3601 ncaattttaa tccccggctt tctgtaaggt ctgatatcca tttattcttc acccaatatc
     3661 ctcgtttgag ccaaagctcc gacaattatc tgggatttga gaacgacctg tccgccaagt
     3721 acaaaccctc taaaaatctg gaaataaact acggtttttc ttatctgatt cctccgaagc
     3781 gggcaggcct gctccccaaa gtaagagatg tgagcaagcc ggcgctctgg agttatttaa
     3841 tggtttcata ccgtttcgaa gtaaagacag gaaaatcaac cagttgagcc ggaggggatg
     3901 aatgcttctc aattataccc ctgcatttgg tacgccaaca gaccgatcca ttctttgatg
     3961 agcagttccc acttgtacag tccgagtttg tcgggcatga ggagcaggtc gaaatcccag
     4021 cgattggcct ccgagatgta gtccactgcg aagggcgtgg cgttcagacc tgccttttgg
     4081 aagcaggctt gtgcgcgggg caggtgccag gcagaggtga tgagcaggca ggaggcgccg
     4141 gggtggcggg tttggatgat gttgttggta aattcagcgt tttcccaggt gtttttcgag
     4201 tcgggttcga ggatgatgtc tgattcgggt acgccccaac gttcgagcag ggcgaagacc
     4261 atttccgcct cgctgggacc gtcttgcaag agcctgccgg aaccgccgct gaggaggatt
     4321 tttttgattt tgcccgaaaa atagagttcg agcgcgttgg tcaggcggtt gccgcgctcg
     4381 ctgagattgt ggcggtcgtg gccgggttcg atgtaaaaat tggagtagcc gcccagtaca
     4441 ataccgatgt cgtaaggttc ggtgatctgg tcggcggtga tggtttcggg ttcccagacc
     4501 cggaagacct gattgctgat ccagcgattg gacatgagca gcaacagcaa caatgccact
     4561 atgatggccc ggcgactgcg gcgcgggtgc ttggtcaaca aggcataagc caacagcgcc
     4621 aacagccaca cgacgggctt gatggtgaag gcgagaactt tggagaggat gaagaacatg
     4681 tatgtaatgt gcagattttt aaaaatgctg gttacgggtt caaggtttcg ggggtagtga
     4741 aggggtgact cggcaagttt cagctccgtt agtcacccct tcactaagca gggcactcac
     4801 ccctcacaca ctcacccctc acacactcac ccctgaaaac cgcctactcc tcccgctcct
     4861 tcccccgtat atactccgcc accggcaccc cctcaaaata caactgctcg atgagcgcct
     4921 tgcctttcag cacgcgcaaa tccactgtga cgggcacttg ccggcggtcg tcccgattca
     4981 gcagttccgc gtaccacttg tcggccaagg gggccaggtc ttcgttcagg tagaaacggc
     5041 gcatctcgtc aggtacaaaa cagtgtatct catggctgct cgtgccggct acgcgggccg
     5101 ccacataatc gggcgtatcg tggggcgggt cggcatgcaa ggcttcaaat tgccccatgc
     5161 ctgcggagtc tttcgtcacg gtgaggaata ccttttggtt gtactccacc aacttccccg
     5221 gcggcgggaa ggccggcata ttgaaattga gttccagata gcggcctctg aacgcatccg
     5281 gcgggtctat gggctgcagc gcgaagcgaa acacagtgcc ctctttcaaa atgccctctt
     5341 gctgccggat catcagcacc ggaatggcga gatacagcac gcacatacct gcaaaagcta
     5401 atgcgtacca ttgattgctt ttcatgatgt gttggatggt tttatcggat ttcaaaatca
     5461 acatgatagt ggtcgtcatg cacgtcgtcc actcttttcg gcaattgtct tgcaaagtaa
     5521 atgtttcttc gctgaagttg ccgccccgaa ggcgttttga acaaatcgtc tttgaggttg
     5581 agtttgaaaa tgaccttgcg aatccgcagc ccgtttgcgc gggccgcatc gtccaccgcc
     5641 agcaagtgtc gggccatact ttcaaaatca atggacgtag ccgcgtcaaa actgagtctg
     5701 ccctccggat caaactccaa taggtaatgc cacacgccga tatggtcgta gcgcctcgtt
     5761 tgtcgccctt tgcgcgtttt cggaaccatg aaatccacgc tcaacccatt gcggtgcgtg
     5821 tagtgaatta gcatctttcc cccttttttt cgcgaacact ccatcagtct gaacactgcg
     5881 cccgggcagg tgcgctcaca cacagcataa gattccttca atgtacggtg tactttagag
     5941 tgggtatatc cgctgcccat cagccaataa ctgattggac tgaagtacct aaaatttttc
     6001 ccgcgatatg gcgtcaacca acctttttcc agatggccat tggcggggct gccactcgct
     6061 atgctctcgc tcttgttgac atgaagccat tgaggggagc ttcccgacaa aatagctgcc
     6121 agaaacaaca tccccaccca ccttcgcttc cttttatgct gcatatattc agcttaatga
     6181 tgacgtctat cataaataga atgagaacgt acccgcacgg tgggcgcggt tattcatgct
     6241 gactcccaac gtaaacgcac cgcagcgccg gcctcctcgc cttgctccag gttccacaag
     6301 cccatgccgt tgttgaaggc atctatattg caactcatcg gttcgacgga gatagagcgg
     6361 cggtgcggcg gcgtgaacac ctgcacaaac cggaacttga ccggcccggc ttcctgcagg
     6421 tagcgaagcc gacctctcgg ccccgaaagc agcacctccg ccacagattc ctcacccgat
     6481 agtgcaaagc aattgtctat cacctgctcg cctatggttt gaagttgctc aaagcgcgct
     6541 tccggcagca gttgcccggt gggaatcatc gtctcgtcaa tttccacctt ccggcagggc
     6601 ggcaattgca ggctcatttc gtccacgctg tcggccaatg tgaaatacgg atgccagccg
     6661 atgcccgccg gaatgggtgt gtcgctgtgg ttgcgtattt tcatgtccat ctcgaagctg
     6721 ccgtcggtgt gcagactgaa agtaataaaa aatgaaaaaa tgaaagggta ggcggcctca
     6781 tcgccgggtt cgtggcgcac acaagttatg gaagcgccct ccgcggtgat gtcggtgcgg
     6841 gctatctgca tgggccggtc tctgccgaag ccgtgtatgg cgttgccggt agcggcattg
     6901 ttgatggcga attggtaggt tttgccttgc cagtgataga ggccgttttt caggcggttg
     6961 ggaaacggat acagcacgat gtttttgccg tatttgtttt ctatcatggc ttccggcgtg
     7021 tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn naagcggtgc gcgccgtcgg ggctgctgat gacgtgtttt
     7141 tggaaaacgc caaatggttc tatggtatgt tgaaacatgg ctcaaggatt ttacagacaa
     7201 acattcataa aaatccttga attaaacaag gtagaaatga aaaaagcctg agggaatcat
     7261 ttttcatttt ttcttgcgca tcccaaaaaa atgattcacc tttgcgcccg tgagcggcac
     7321 acgaccagct ccctccgaat ccccccaggg cggaaacgca gcaagggtag gcaggttgag
     7381 cggtgcgatg tgttcgctca cacctttttt taccccattc cgttctttct ttcaaagttt
     7441 gttttatctt tgcgacacaa ttcgtcgcca tgcagttttt tatagataca gccagcttgg
     7501 atctaatcaa agaagcacgc gatttgggca tccttgacgg cgtgactacc aacccctccc
     7561 tcatggccaa agaaggcatc actggtgagg ccaatattct gaaacactac gaaaaaattt
     7621 gtaaattggt ggacggcgac gtcagtgcgg aggtcatctc caccgatttc gacggcatca
     7681 cggccgaggg caaaaggctc tcgctcatcg ctcccaatat cgtggtaaaa gtgcctatga
     7741 ttaaggaggg cgtcaaagcc atgagttatt tccgcgacaa cggcatccgc accaattgta
     7801 cgttggtgtt ttctgccgga caggccattc tggcagccaa ggcgggcgct acttatgtct
     7861 ctcccttcat cggtcgcata gatgatgttt cttgggacgg catggagttg attcgccaaa
     7921 ttgcggaaat ctatggtatt cagggcttcg aaacgcgcat tctggccgcc tccatccgca
     7981 atggaaagca tattgtggac gctnnnnnnn nnnnnnnnnn nnnnnnncgg cgcagatgta
     8041 gtgacctgtc ctttgtcttc gattctgggt ttgctcaagc acccgctcac cgacatcgga
     8101 ttggagcagt ttctggccga tcaccgcaaa gcagaggccg tttctaaata agtattttat
     8161 cagccattgc agccgctcgt tttttcggcg gctccatcat acgcagaggc tttctacata
     8221 taccaattat gcgaggggta gcatcacgct acccttcgtt ttttacgccc gtttgcttca
     8281 acacccctac ggtaatggca tattgcgccg acaggtaagt gctcatcacc aacagccgcg
     8341 caaagggcag ttctgcataa aacttatccc aggccagcaa actgtcggag gccacaaaca
     8401 gcaccgcgcc gcctatgatc cacagcgccg ctcttttcga ccaataagaa taacccgcca
     8461 tggccagcgc caacatgctg ccgatgacga gaccgtaaac ggctaccgga atctgcaaag
     8521 ctccggccac cggcaataat tgatacaaaa accagcccag atacgccgcc agcagcgccc
     8581 agccggcata catcagcggc ctgcttcttg ggctgcccga tatgagcttg taaaannnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 ngtctcctgc aaaggaaaaa aacagcgccg ccatcagcag gtttttgcct gaaaaggcac
     8761 gggtattcca tactgcattg agtaacatgg gtaccagcag cgccttgaaa attatgcggg
     8821 cagagtccgg caagaagtaa ataccagcca ggtaaagcac ggcggcaatg agataaatgc
     8881 gaagttgatt tgaaggagac ataattataa aagtattgat
//
LOCUS       DAORLF010000173         8905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4245_length_8905_cov_1.762938, whole genome shotgun sequence.
ACCESSION   DAORLF010000173 DAORLF010000000
VERSION     DAORLF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_8905_cov_1.762938"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK198_13970"
     CDS             <1..227
                     /locus_tag="PK198_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HRF39891.1"
                     /translation="HRDFQRDYHAFKGNAYGLANTLLQTAFLKPKLKSKKVANLYYTG
                     QLTTPGPGMPPSLISGQVVAREVCREFLQG"
     assembly_gap    263..387
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <388..1167
                     /locus_tag="PK198_13975"
     CDS             <388..1167
                     /locus_tag="PK198_13975"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRF39892.1"
                     /translation="YSTSFSLGMRVFSPKYREPVYSIYGFVRFADEIVDTFHGYPKAE
                     LLQRFREDTRRAIEERISLNPVLHSFQEVVHRYQIENELIDAFLDSMEMDLHFHTYGD
                     HLYKKYIYGSAEVVGLMCLRVFCEGNDAEYQRLKPAAQSLGSAFQKINFLRDMASDFK
                     ERGRVYFPGVDFTQFTEADKIAIEADIKKDFDDAYFGILQLPQGVRLGVYMAYIYYIK
                     LFQKIKKAPAQQVTKERIRVPDGRKIVLLLGSAVRHQFNML"
     gene            1167..1823
                     /locus_tag="PK198_13980"
     CDS             1167..1823
                     /locus_tag="PK198_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRF39893.1"
                     /translation="MEKQPAAKYERIFAGAMIFLFASGLVAHAVPSLRPLTASTTDLF
                     LLLINGTLLWLIVRNNSDNRLWFWSLSVYVFTFTVEAVGVATGAVFGEYAYGAGMKVQ
                     WIGVPLVIALNWTLLTLGVNDLASRITSSPWLRAAAAALLIAAYDWFIEPVAIGLDYW
                     QWATGSDIPVQNYVAWAVVAFAASLPLHLLRIRFRHWLLPLYLGVQWFYFVLMGWVGI
                     "
     gene            complement(1824..3599)
                     /locus_tag="PK198_13985"
     CDS             complement(1824..3599)
                     /locus_tag="PK198_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39894.1"
                     /translation="MKTNTLHLLPASLLVLMLLQSCQPSDTAETTFKHHPHDPFRETM
                     VESQIFELNASQDNVTEGKHETLLVFPKGCFVNAEGKAVTGKVKVELAEAFSLSRMLL
                     SNLTTTSDSALLETGGMIFLNVTGNGEQLYISKSNPVYIEIPAPHKVPGMMAYKGTRD
                     ATGNMNWTEPKTLEDFLVPIDFSLLDFLPKGFDDEVVISMPFAGRQKADRALADSLYY
                     SLSVRDGKEWLGKMADVNLNEPNDNSTQSVGNQIDGDTVDEETAYCGIDPAIIQAIRS
                     EEFQKTFIATREFEKRLQTIFSTCRDDIIEIYIKNTDKNLWESDSLAAVALQGDALRR
                     EFLRFHQWKQTNVDGAGKYGALLRQYYEQRLSAVKSSLEVARDKAIRAHREKNEKAIK
                     TLETYKELLRKREAYRMETYGFIWSETGWINIDRGIAPKNFEWGKLEVAVQNGKAFDR
                     VHTYVVYQSIRSLYRLNTMEQEVFFTGEQTDRGMWMPKKQPATCIVIAYKDEEPYWAV
                     NEFITGPGERLALTLSPTKYEDLNVALRAYDSYGQENNINEDLKNMALLAEDDKRRAV
                     LRSELAVISRLRRIAYPCCAVVEKN"
     gene            complement(3814..>4264)
                     /locus_tag="PK198_13990"
     CDS             complement(3814..>4264)
                     /locus_tag="PK198_13990"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRF39895.1"
                     /translation="SLLYSLILPGAGQVYNGRWWKVPFVYGALGATVGVYLYNQENYK
                     NFQLAYSARLAGDPVPSGLPPEFSNPVLQELRTLRVYRDRYNKDRQSWLIYSIGVYGL
                     QAIEALVDAHLRDFDVNEDLSLRLKPSLGMPGPGGMTPGVSLTLTIR"
     assembly_gap    4265..4283
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4517..4526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4527..>5268)
                     /locus_tag="PK198_13995"
     CDS             complement(<4527..>5268)
                     /locus_tag="PK198_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRF39896.1"
                     /translation="IEEKQEEVVKELSHTVAMIPVDQIEVNPWQPRNEFDETALAELS
                     ESVKVHGLIQPITVRRLSPNAYQLISGERRLRASKMAGLIEVPAYIRLANDQEMLEMA
                     LVENIQREDLNAIEVAITLQRLKEECSLTDESLSDRMGKKRSTVTNYLRLLKLPPNIQ
                     KSIKDREISMGHARVLAGIDDYAVRDSLYRQTIKEGLSVRDLEHLAAALSEDRPKKAP
                     KERLPDAYDDVQKKLRELFGTAKVQIKLQ"
     assembly_gap    5269..5409
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5458..>5896)
                     /locus_tag="PK198_14000"
     CDS             complement(5458..>5896)
                     /locus_tag="PK198_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRF39897.1"
                     /translation="YDYIFIDCLPSLGLITINALTAADSVLVPVQCEYFALEGLSKLQ
                     NTINLVRKKLNTKLEIEGILLSMFDSRLRLSNVVVAEVREIFKGKVFETIIHRNSRIG
                     EAPNMHQPVVMLNAGSKGSVNFLNLANEFLANNNDKISAPQPA"
     assembly_gap    5897..5961
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5962..6177)
                     /locus_tag="PK198_14005"
     CDS             complement(<5962..6177)
                     /locus_tag="PK198_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF39898.1"
                     /translation="MAILDKKVLLIDADPQANSSSGVGILEGDTDTNLYDCMVNGMDP
                     MDALYETDTPNLHLMPSHINLVGAELEL"
     gene            complement(6303..>6631)
                     /locus_tag="PK198_14010"
     CDS             complement(6303..>6631)
                     /locus_tag="PK198_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF39899.1"
                     /translation="GGNPGGFVVWNADQCFYFAGDTALTQDMSFIPMTCPKLDVAILP
                     IGDNFTMGYEDAAIAAGLIECDRIIACHYDTFGYIKIDHEAAKKAFSDRGKKLILLPV
                     GGSTEI"
     assembly_gap    6632..6692
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6693..6987)
                     /locus_tag="PK198_14015"
     CDS             complement(<6693..6987)
                     /locus_tag="PK198_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF39900.1"
                     /translation="MKITYYGHACFGVQLGESQLLFDPFISPNGLASHIDVSDIQADV
                     ILVSHGHGDHVADVESIAKRTGAFVVSNFEIVSWFENKGLKGHPMNHGGKWNFD"
     gene            complement(7049..>7856)
                     /locus_tag="PK198_14020"
     CDS             complement(7049..>7856)
                     /locus_tag="PK198_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRF39901.1"
                     /translation="LGSVLNVSVGDLFAAAFVPGFMLVGLYLVYILVVSALNPSVAPA
                     MPPEEVAAFIGPGFGAKVLKALVMPLVLIVAVLGSIFAGIASPTEAAALGAIGATLLT
                     ALQKKLNMNVLRHVMRETTHLTAMVFIILLGATTFSLVFREMNGDRYLVEWVQNANLS
                     PYMFLMLVMLVVFVAGFFIDFIEIIFIIVPIAAPIFKALGMDMIWIGVLLTINLQTSF
                     LTPPFGFSLFYLKGVAPPEVRTAQIYRGIVPFLIIQLLLLALVMFFPGIL"
     assembly_gap    7857..7922
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7923..8341)
                     /locus_tag="PK198_14025"
     CDS             complement(<7923..8341)
                     /locus_tag="PK198_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRF39902.1"
                     /translation="MEILALILFISIFVLILYGYPVAFTLAGLSLLYAFCFLEPSVLA
                     ALPARVMGVMNNYVLIAVPLFIFMGLMLERSGLAGQLLETMAVLMGRIRGGLAVSVVL
                     VGALLAASTGIVGATVVTMGLISLPTMLKRNYRTELAT"
     gene            complement(8357..8905)
                     /locus_tag="PK198_14030"
     CDS             complement(8357..8905)
                     /locus_tag="PK198_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRF39903.1"
                     /translation="MDKFLLQTYRKISRLNARIGAATAWLTAALVLLVGVDVLYRRVL
                     QDTQTWIMEMEWHLFALIFLLGAGFAFRHDKHVRVDLFYSKFSAKDKAWVNLIGGVLF
                     LLPWCAALMVSAWEFAADAFRIRESSPDPGGLPARYLIKFSIVLGAFFLFLQGIASVA
                     EAILHLRGIATEADAPDLPESI"
BASE COUNT         1995 a         2210 c         2142 g         2071 t
ORIGIN      
        1 cgcaccgcga tttccagcgc gattaccacg cgttcaaagg caatgcctac ggattggcca
       61 acacgttgct gcaaacggct tttctgaaac ccaaactgaa aagcaaaaaa gtggccaacc
      121 tctactacac cggccagttg acaactccgg ggccgggcat gccgccctcg ctcatcagcg
      181 ggcaggtggt ggcgcgggaa gtgtgcaggg agtttttgca ggggtgagta tgtgaggggt
      241 gagtatgtga ggggtgagta tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnntac agtacgtcct tttctctggg tatgcgggtg
      421 ttttctccca agtaccgcga gccggtctat tctatctacg gctttgtccg ctttgccgac
      481 gagatcgtag atacctttca tggctacccg aaagccgagc tgctccaacg gttccgcgaa
      541 gatacccgcc gcgccattga ggagcgcatc agcctcaatc cggtacttca ttcttttcag
      601 gaagtagtgc atcgctacca gattgaaaat gagctgattg atgctttttt ggacagtatg
      661 gaaatggacc tgcatttcca cacctatggc gatcatttgt acaaaaaata catttatggc
      721 tcggcagaag tggtgggatt gatgtgcctg cgggtcttct gtgagggcaa cgacgccgag
      781 taccaacgcc tgaaaccggc ggcgcaaagc ctcggttcgg ccttccaaaa aatcaacttc
      841 ctgcgcgaca tggcctctga ttttaaagag cgcggcaggg tctatttccc cggagtggac
      901 ttcacgcagt tcacagaggc cgataaaatc gccattgagg ccgacatcaa aaaagacttc
      961 gacgatgctt actttggcat cctccaattg ccgcagggcg ttcgtctggg ggtttatatg
     1021 gcttatattt actacataaa gctcttccaa aaaatcaaaa aggccccggc acagcaagta
     1081 actaaggagc gcattcgcgt acccgacggg cgcaaaatcg tgctgctttt aggctctgcc
     1141 gttcggcatc agttcaatat gttgtaatgg aaaaacagcc tgctgctaag tacgagcgca
     1201 ttttcgccgg cgccatgatt tttctcttcg cttccggctt ggttgctcat gccgtgcctt
     1261 cgctgcggcc gctcaccgct tccacaacgg atttattttt gctgctcatc aatggtactt
     1321 tgttgtggtt gattgttcgc aacaactcag ataatcgcct gtggttctgg tctttatccg
     1381 tgtatgtttt tactttcacg gtagaggcag tgggcgtggc tacgggcgct gttttcggcg
     1441 agtatgctta tggcgcaggc atgaaagtgc agtggatcgg cgtgcccttg gtcattgccc
     1501 tcaactggac gctgctcacg ctgggcgtca acgatctggc ctcccgaatc acgtcttcgc
     1561 cctggctgcg ggcggcggcg gcggccctgc tcattgcggc gtatgattgg ttcatagagc
     1621 cggttgccat tgggttggac tactggcagt gggctaccgg cagcgatatt cctgtgcaaa
     1681 actatgtggc atgggcggtc gtagcttttg cggcctcgtt gccgttgcac cttttgcgca
     1741 tccggtttag gcactggctg ttgccgctgt atctcggcgt gcagtggttc tattttgtgc
     1801 tgatggggtg ggtggggata tgattagttt ttctcaacga cggcacaaca tgggtacgca
     1861 atgcggcgga gcctgctgat aaccgccaac tctgatctga gtacggccct tcgcttgtcg
     1921 tcctctgcca acagcgccat gtttttcaag tcttcattga tgttgttctc ttgtccgtag
     1981 gaatcgtaag ccctcagcgc tacattcaaa tcctcatatt tcgttggcga taaagtcaat
     2041 gcaagccgct cgccgggacc tgtaataaat tcatttacag cccaataagg ctcttcatcc
     2101 ttgtaagcaa tgacaataca agtcgccggc tgctttttgg gcatccacat tcctctgtct
     2161 gtttgttctc cggtaaaaaa cacttcctgc tccatagtat tcaaacgata gaggcttcgt
     2221 atagattgat aaacgacgta agtatgcacc ctgtcaaagg ctttcccgtt ttgaaccgcc
     2281 acttcgagtt ttccccactc aaagtttttg ggagcaatgc ccctgtctat gttgatccag
     2341 cccgtttccg accagatgaa accataggtc tccatcctgt atgcttctct ttttctgagc
     2401 agttccttgt acgtttctaa tgtttttatg gccttctcat ttttttctcg atgcgcccgg
     2461 atggccttgt ctcgcgcgac ttccaatgaa gatttgacgg cactcaatct ttgctcgtag
     2521 tattggcgca agagggcgcc gtacttaccc gcgccatcca cgttggtctg tttccattgg
     2581 tggaagcgca gaaattcgcg ccggagtgca tcgccctgca aagcgacagc cgccaaacta
     2641 tctgattccc agaggttttt atccgtgttt ttgatgtaaa tttcgatgat atcgtctcgg
     2701 caagtcgaga agattgtctg aagtcgtttt tcaaattcgc gggttgcaat gaacgttttt
     2761 tgaaactcct cagatcgaat ggcttgtatg atggcaggat caataccgca gtaggctgtt
     2821 tcttcatcga cggtatctcc atcaatttga ttaccaaccg attgtgtgga attatcattc
     2881 ggctcattca agtttacatc tgccatcttt cccaaccact cctttccatc ccgcaccgac
     2941 aagctgtaat acaggctgtc ggccaatgcc cggtcggcct tctgacgccc tgcaaaaggc
     3001 atagatataa ctacctcatc atcaaatcct tttggcaaaa aatccagcaa actgaaatca
     3061 atcggcacga gaaaatcctc caatgttttc ggctccgtcc aattcatatt tcctgttgca
     3121 tctctggttc ctttgtaggc catcatgccg ggcactttat gcggagcggg gatttcgata
     3181 tacacagggt tgcttttgct gatgtatagt tgttctccgt tgcccgttac attcaaaaaa
     3241 atcattccgc cggtttcgag cagcgcgcta tccgaggtag ttgtgaggtt ggaaagcagc
     3301 atccggctca gggaaaaggc ctccgccaat tccactttca cctttccggt tacggcctta
     3361 ccctcggcat ttacaaaaca tcctttcgga aaaaccaaca gggtttcgtg tttgccctcc
     3421 gtcacattgt cttgggaggc gttcagttca aagatttggc tttccaccat cgtttcccgg
     3481 aaggggtcat gggggtgatg cttgaatgtc gtttcagcag tatctgacgg ttggcaactt
     3541 tgcagcaaca tcagtaccag caggctcgcc ggcaggaggt gtaatgtgtt ggttttcata
     3601 gataaaacat tactattcag cgggtctaca aaagctacga gctatgagtc acgagctatg
     3661 agtttgttta ttcgttgaaa aattagattt tccatttggg ggaatttcaa agcctcatct
     3721 tgaaaaaagg ctctatttca ttttgggaga tcaaactcgt agctcgcggc tcatggcttt
     3781 ttgggaaagc ccctgaatag tcacgataaa acatcaacgt atcgtcagcg tcaacgaaac
     3841 tcccggcgtc ataccgcccg gtcccggcat acctaagctt ggtttgaggc gcaggctgag
     3901 gtcttcgttc acgtcaaaat cgcgcaggtg tgcgtccacc aatgcctcaa tggcctgcaa
     3961 accatacacc ccgatggaat aaatgagcca gctttggcgg tctttattgt agcgatcgcg
     4021 atatacccgg agcgttcgga gttcttgcag aacaggattg ctgaattcgg gcggcaatcc
     4081 agagggtacc ggatcgcctg ccagccgggc cgaatacgcc aactggaaat tcttgtaatt
     4141 ctcctgattg taaagataca cgcccaccgt agcgcccagc gccccataca caaaaggcac
     4201 tttccaccaa cggccattgt aaacctgccc ggcgcccggc aaaatcaaag agtacaggag
     4261 cgacnnnnnn nnnnnnnnnn nnntcgggca gtcttttgac tttcggagta tcggtagagt
     4321 ccaggcccaa gctcatttct tcttcccaaa gcgattctgt ggtttcggca ggagtagccg
     4381 aaagagacgg accacccagc caacaacagg ccgaaagtaa gaagaggatg cgcagtttat
     4441 ttctcaaggt gttccaaata tgcgttgaac tgatctaccg tatcgaacgg aagaacgatc
     4501 tgtcccttgc tctggcnnnn nnnnnnttgt aatttaattt gcactttggc ggtgccgaac
     4561 aactcgcgca gttttttctg cacgtcgtcg taggcatccg gcaggcgttc tttcggcgct
     4621 ttttttggtc tgtcttcgct cagcgcagcg gccaagtgct ccagatcgcg caccgaaagc
     4681 ccttctttga tggtctgcct gtacagcgaa tcgcgcacgg catagtcgtc tataccggcc
     4741 aaaacgcggg catggcccat gctgatctcg cggtctttga tggatttttg gatattgggg
     4801 ggcaatttca gcaggcgcag gtagttggtc acggtgctgc gctttttgcc catgcggtcg
     4861 gagagcgact catctgtgag actgcactct tccttgaggc gttgcagggt gatggccact
     4921 tcaatggcgt tgaggtcttc gcgctgaatg ttctccacca gcgccatttc cagcatttcc
     4981 tggtcgttgg ccaatcgaat gtaggccggc acttcgatga ggcccgccat tttggaggcc
     5041 cgcaaacgac gttcaccgga gatgagctgg taggcattgg gcgaaagcct gcgcaccgtg
     5101 atgggctgaa tgagaccatg caccttcacc gattcggaga gttcggccag ggcggtttcg
     5161 tcaaattcat tgcgcggctg ccaggggttc acttctatct gatccacagg aatcatcgcc
     5221 acagtgtgcg aaagttcttt caccacttct tcctgtttct cctcaattnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnt aagggtttgt accggcaaga tgaaaaaata aaggttcccg acaccggtta
     5461 tgccggttgc ggagcggaaa ttttgtcgtt gttgttggcc aggaactcat tggccaagtt
     5521 caggaagttg acactgccct tgctgcctgc attcagcatc actaccggtt ggtgcatgtt
     5581 gggcgcttcg ccaatgcggg agttgcggtg aatgatggtt tcaaacacct tgcctttgaa
     5641 aatctcgcgc acctcggcca ctaccacatt ggaaaggcgc aggcggctgt cgaacatgga
     5701 cagcaggatg ccttcgattt ccaatttcgt attcagcttt ttgcgcacta agttgatggt
     5761 gttttgcagc ttgctcaagc cttccagcgc aaaatactcg cactgcaccg gcaccagcac
     5821 cgagtcagcc gccgtcaggg cgttgatggt gatgagaccc agcgagggca ggcaatcaat
     5881 aaaaatgtag tcgtacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn ncaattccag ttctgcgccc acgaggttga tgtgcgaagg
     6001 catgaggtgg aggttgggcg tgtcggtctc gtagagcgcg tccatggggt ccatcccgtt
     6061 gaccatgcag tcgtacagat tggtgtctgt atcgccttcc agaatgccca cgcccgacga
     6121 ggagttggcc tgcggatcgg cgtcaatgag cagcactttt ttatctaaaa tggccaaact
     6181 ggcgctcagg ttgatggcag tggtggtttt gcctgatgtt ttatcgtaaa cgtgaaaagg
     6241 tgaagcgatc agaaaataag atcaggctgc accttttgaa aaggaagtct gcggcgaaaa
     6301 aactagattt ctgtgcttcc gccgacgggg agcaaaatta gcttttttcc cctatccgag
     6361 aaagcttttt ttgcggcctc gtggtctatt ttgatatagc cgaaggtatc gtaatggcag
     6421 gcaatgatgc ggtcgcactc gatcagaccg gctgcaatgg cggcatcttc gtagcccatg
     6481 gtgaagttgt cgccgatggg caaaatggct acatccaact tcgggcaggt catgggaatg
     6541 aaactcatgt cctgcgtcag cgcagtgtct ccagcaaaat agaagcattg gtcggcattc
     6601 cagaccacaa aaccgccggg gttgccgccg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatcgaaa ttccacttgc cgccgtgatt
     6721 catcggatgc cctttgaggc ccttgttttc aaaccaggaa acgatctcga agttggaaac
     6781 gacaaaggcg cccgtgcgtt tggctatgct ttccacatcg gccacatgat cgccgtgccc
     6841 gtgcgacacc aaaatgacat ctgcctgaat gtcagacaca tctatatgtg aggctaaacc
     6901 gttggggctg ataaacggat cgaacagaag ttggctttcg cccaattgca caccaaaaca
     6961 ggcgtgacca taataggtaa ttttcatggc tgctccgatt ttggcgcaaa ggtagggctt
     7021 tgaggcgaat tttcggggcc attttcaact ataagattcc cggaaaaaac ataaccaatg
     7081 ccagcagcag cagttgaatg atcaaaaacg gcacaatacc cctgtatatc tgcgccgtgc
     7141 gaacctccgg cggggccacg cctttgagat agaacagcga aaacccgaag ggcggggtca
     7201 gaaaagatgt ttgtaaattg atggtgagca acacgcctat ccagatcata tccatcccca
     7261 gcgccttaaa aatgggggct gcaatgggca caatgatgaa gatgatttca ataaaatcta
     7321 taaaaaaacc ggctacaaat accaccagca tcaccaacat caaaaacatg tagggcgaga
     7381 gattggcgtt ctgtacccac tccaccaaat agcggtcgcc gttcatctct ctgaaaacca
     7441 acgagaacgt tgttgctccc agcaagatga taaaaaccat agccgtgagg tgggtggtct
     7501 cgcgcatgac gtggcgcaat acgttcatat tcagcttttt ttgcaaagcc gtcagcagtg
     7561 ttgctccaat agctcccagc gccgccgctt ccgtaggcga tgcaatgccc gcaaaaatag
     7621 accccaatac ggccacaatg agcaccaatg gcatcaccaa ggctttcaac acttttgctc
     7681 caaaacccgg ccctataaac gcagctacct cttcgggcgg catcgccggg gccacagagg
     7741 ggttgagtgc cgaaacaacg agaatgtaaa ccagatacag ccccaccagc ataaatccgg
     7801 gtacaaacgc cgcagcaaac agatcgccca cagatacatt gaggacgctg cccaacnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nngtggccaa ttcggtgcgg taattccgct tgagcatggt gggcagacta atcaggccca
     7981 tcgtaacaac cgtagcccct acgatgccgg tggatgccgc cagcaacgcg cctaccaaca
     8041 ccacagaaac cgccaacccg ccccgaatcc gccccatcag cacggccatg gtttctaaca
     8101 gttgccccgc cagccccgac cgttcgagca tgaggcccat aaaaataaac aacggcacgg
     8161 cgatgagcac ataattgttc atcaccccca tcacccgtgc cggcaaggct gccaaaacgc
     8221 tcggctctag aaaacaaaag gcgtacagca gcgaaagccc cgccagcgtg aaagccaccg
     8281 ggtagccgta caaaatgagg acaaaaatgc tgatgaaaag tataagcgct aaaatttcca
     8341 tgccaagtgc gggttttcaa atgctttcag gaagatcagg tgcgtcggct tccgtagcga
     8401 tgcccctcag gtgcaggatg gcctcggcaa cggaagcaat gccttgtaaa aacagaaaaa
     8461 acgcgcccag tacaatggag aatttgatga ggtaacgggc gggcaatcca cctggatcgg
     8521 gcgaagattc tcgtatgcga aaggcatcgg cagcaaattc ccatgcagaa accatgagcg
     8581 ctgcacacca gggcaacaaa aacaacaccc ctccgatgag attgacccag gctttgtctt
     8641 tggcgctgaa tttcgagtaa aacaagtcca cccgcacatg cttatcgtgg cggaatgcaa
     8701 aaccggcgcc caacaaaaag atgagcgcaa acaggtgcca ttccatctcc ataatccagg
     8761 tctgcgtatc ctgaagcacc cttcgataca atacatccac gcctaccagc agcaccaaag
     8821 ccgccgtgag ccaggcagta gcggctccga ttcgggcgtt gagccgggag attttgcggt
     8881 acgtttgaag taaaaatttg tccat
//
LOCUS       DAORLF010000174         8892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4255_length_8892_cov_2.015842, whole genome shotgun sequence.
ACCESSION   DAORLF010000174 DAORLF010000000
VERSION     DAORLF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_8892_cov_2.015842"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PK198_14035"
     CDS             complement(<1..879)
                     /locus_tag="PK198_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRF39904.1"
                     /translation="MPEVEFAQVTEPTTAPDLSCYHCGEECPDDHIHIEEKAFCCEGC
                     KMVYEILNTNGMCQYYDLDEKAGVSLRGKTRAEFAFLDDPEVKERLINYTDGELTRVT
                     FYLPQMHCASCIWLLENLYRLNEGVRNSSVNFPKKEVYISYSESKTTLRKVVELLSDI
                     GYEPAITLSDLDNEEKPLVNRSFYYKLGVAGFAFGNIMLLSFPEYLGLEKETDGFFYL
                     LFGYLNILLSIPVVLYSGRDYFTSAWTGLKQRQLTIDVPLALGIAVLLFGKRLWQETR
                     IPLFEQGVALPGGARGD"
     gene            complement(962..2500)
                     /locus_tag="PK198_14040"
     CDS             complement(962..2500)
                     /locus_tag="PK198_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRF39905.1"
                     /translation="MIINATPTEVEIALMENGRLVELHYQKTNSNFSVGDVFLGKVQR
                     LMPGLNAAFVDIGHKKDAFLHYTDLGPKLRSLLKYSAGVTAGNISTHKLDNFKLEPEI
                     VKTGKVDQVLEKRQNILVQILKEPISTKGPRLSCEVTIPGRYLVLTPFNEVVAVSKKI
                     GNAEERKRLQNLVESIRPKNFGVIVRTAAEGKKTAELHEELAFMMSKWEDIYQQMYKA
                     TPPAKLLSELDKTSSILRDLLTDSFNRIVVNDKELFQNIKNYLATIAPDQIKILSLHK
                     GSRPIFETFSVDRQIKASFGKTATLSSGAYLVIESTEALHVVDVNSGHKMSNAAADDA
                     ILNVNLEAAEEIARQLRLRDIGGIIIIDFIDMKNPDHKKDLHLKMREFMKKDRAQHTV
                     LPLSKFGLMQITRQRVRPEVKINTAEVCPTCNGSGKINASVLLMEDIQRDLDFVLQSQ
                     SKAKLTLSVHPYIEAYLKRGFFNQQIRWFRQYFRWIKIKPSNQLAMTQYKFYDEHNDE
                     IRLS"
     gene            complement(3120..3887)
                     /locus_tag="PK198_14045"
     CDS             complement(3120..3887)
                     /locus_tag="PK198_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39906.1"
                     /translation="MVSATILFFALYFGCETQSKDQQSLEKTRVFSAESTDVNVLMKE
                     AQGRLQANSKNEIGSIEHELELVQDDSSAYTSTLMRLAGAWFNAGEPAISGHYAQLVA
                     QRLQTEDAWSKAGTTYAICLQRSEDEKVRTYCTQRAVAAFESAISVNPENVAHRVNLA
                     VVHAENPPKEDVMRGITMLLELNRQYPENVSVLNNLGRLAIQTGQFDKAVQRLERVLE
                     IEPGNLTATCLLADAWNGAGDAAKAEQFAKACGQLNE"
     gene            complement(3938..4246)
                     /locus_tag="PK198_14050"
     CDS             complement(3938..4246)
                     /locus_tag="PK198_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF39907.1"
                     /translation="MRKADLVNAISEKTGVAKVDVLVALETFFKEVKGSLAGGENVYI
                     RGFGSFVIKRRAKKIGRHIKRNEAIEIPEHFIPSFKPAKVFVDQVKNNVTEMPEEEED
                     "
     gene            4494..5207
                     /locus_tag="PK198_14055"
     CDS             4494..5207
                     /locus_tag="PK198_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39908.1"
                     /translation="MKKQATHRMYNVPDAELYVQCMERIRYAYRDIDRFVPYSYDLER
                     LRAFGALCERFNAMPDDDEMVGDQMLMTEKKDDAAERLKTAIRSLMTRVEIKFNNRSG
                     RYRKFGTAKMGDMSDAQLLFCGRRVARVARQQMDFLADTGMNETIIQRVTDACRDFET
                     ALNIQQDRIADRDIAVENRTEQGNKMYQELVVLCNIGKDIWADTDPVKYENYCIYESN
                     NHQKIARKEREKSAVASKE"
     gene            complement(5214..>6423)
                     /locus_tag="PK198_14060"
     CDS             complement(5214..>6423)
                     /locus_tag="PK198_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF39909.1"
                     /translation="VAATGILQNPTEIGHGYCRPEEAPKNLPRRLQWHFKAENVNDFV
                     WAADPDYEHTALTRADGTVLRFFYQPGEATQAWSRLPAIMDRAFDYINARFGQYPYPE
                     YAFIQGGDGGMEYPMATLITGNRPLNSLVGVSVHELMHCWYPMVLSTNEARYAWMDEG
                     FVNFASSEVMNHLAGEKLLPGRSVSNMPHKPDYQGYVNIALSDWEEALSTPSDHFQTN
                     TAYGVASYNKGSVFLSQLSYLIGLEAFNAGLLRYFDTWKFKHPDDKDFIRVMERASGL
                     ELDWYLDYWVYSTHTIDYIVAAVEADRSKSSIIRLHRKGAMPMPVDVEITLNDGQTMR
                     YHIPLCMMRGGKPSDSGWDGFEKTEAWPWVEPYYQWSVPVRHKDIAKVVIDPTGRMAD
                     VNRANNEWKP"
     assembly_gap    6424..6451
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6452..6737)
                     /locus_tag="PK198_14065"
     CDS             complement(<6452..6737)
                     /locus_tag="PK198_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF39910.1"
                     /translation="MRQNDQELRNWTVRGTILEVSLNEPLMPGETVKLDMTFESQVPV
                     QIRRNGRNNAEGISYSMAQWYPKVCAYDRHGWHADDYIGREFYGVFGDFNV"
     assembly_gap    6784..6798
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6799..7080)
                     /locus_tag="PK198_14070"
     CDS             complement(<6799..7080)
                     /locus_tag="PK198_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HRF39911.1"
                     /translation="MTKMIRAVSLTALLYSALMASGQPGTGYWQQRVNYDMSIGFDTK
                     KHRFDGEQVLHYHNQSPDTLHRVFYHLYLNAFQPGSMMDMRSRNISDSDS"
     gene            complement(7101..7244)
                     /locus_tag="PK198_14075"
     CDS             complement(7101..7244)
                     /locus_tag="PK198_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39912.1"
                     /translation="MEGVDTTSAGYQIGHQIGSWLPFAVLAILFIIMLRAVLRKQRDT
                     PNI"
     gene            7437..8147
                     /locus_tag="PK198_14080"
     CDS             7437..8147
                     /locus_tag="PK198_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HRF39913.1"
                     /translation="MDIQRKAADWAGELIKDRFMENNIHSERNKHAETIIRNHILWSM
                     GASFLIPIPVADIFAVSALQLDMIRQLCRVYNVDFAETQGKAVVTSLTSSTLAKVGAR
                     SLIKIIPGIGQLVGGVATSVFNGASTYALGEVFKKHFESGGTILDFDTDRLKKFYQEK
                     FEKGKKVAEQLKKDQEAKKAAENAAPAAASGPSAPSPAAAQPSAADRLRELAELKNQG
                     IISEEEFEQMKKRVIESF"
     gene            8595..>8892
                     /locus_tag="PK198_14085"
     CDS             8595..>8892
                     /locus_tag="PK198_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRF39914.1"
                     /translation="MLLDLHHLREEYAERKLDASEAHTDPFIQLQQWLNDALMAELPE
                     PNAMTLATCTKEGIPSARVVLLKGVDRSGLDFYTNYHSRKGREIDENPHVAVTFL"
BASE COUNT         2167 a         2131 c         2180 g         2371 t
ORIGIN      
        1 gtccccgcgc gccccgcccg gcagggccac accctgctcg aatagcggga tacgggtttc
       61 ctgccacagc cgcttgccga acagcagcac cgcgatgccc aatgccagcg gcacgtcaat
      121 ggtgagttgg cgttgtttca gccccgtcca tgccgaggtg aaataatctc tgccgctgta
      181 cagcactacg ggaatgctga gcaggatatt gagatatcca aaaagcaggt aaaaaaagcc
      241 atcggtctct ttctccaatc ccaggtattc cgggaaactg agcagcatga tgttgccgaa
      301 agcgaaacca gctacgccga gtttgtaata aaacgagcga ttgaccaagg gtttttcctc
      361 attgtccaaa tcgctgagcg tgatggccgg ttcgtagccg atgtcggaga gcagttccac
      421 cactttgcgc agggtggttt tcgactccga ataggagata tagacttcct tttttgggaa
      481 attgaccgat gaattgcgca cgccctcgtt gagccggtag aggttttcga gcagccagat
      541 gcaggaagcg cagtgcattt gcggcaggta aaacgtgacg cgggtgagtt cgccgtcggt
      601 gtaattgatg aggcgctcct tcacttcggg gtcatcgagg aaggcaaatt ccgcgcgggt
      661 tttgccccgc aggctcacgc cggccttctc atcgaggtcg taatactgac acattccatt
      721 ggtattcagt atttcataca ccattttgca accctcgcag caaaaggcct tctcctcaat
      781 gtggatatga tcgtcgggac attcctcgcc acagtggtag caggaaagat cgggtgcggt
      841 ggtcggttcc gtcacttggg cgaattctac ttcaggcatg ttcatcgttt tggccaccca
      901 aaggtagccc ggcgctgaaa tggcctccga tgataaatgt cactgcctct tttgattgtg
      961 gtcaactcaa ccgaatctcg tcgttgtgct cgtcataaaa cttgtactgc gtcatagcca
     1021 actgattgga tggtttgatc ttaatccaac ggaaatactg tctgaaccag cgaatttgct
     1081 gattgaagaa gccccgcttc aggtatgcct ctatatacgg atgcacggaa agcgtgagtt
     1141 tggccttgga ttgcgattgc aacacaaagt ccagatcgcg ttgaatatct tccatcagca
     1201 acaccgaagc attgattttg ccgctgccgt tgcaggtggg acacacctcg gcggtgttga
     1261 ttttgacctc cgggcgtacg cgctgccggg tgatctgcat cagcccgaac ttgctcagcg
     1321 gcagcacggt atgttgagcg cggtcttttt tcataaactc gcgcatcttc aggtgcaggt
     1381 cttttttgtg gtcggggttt ttcatatcaa tgaaatcaat gatgatgatt cccccgatgt
     1441 cgcgcagacg gagttggcgg gcgatttcct cggcagcttc taagttgacg tttagaatgg
     1501 catcgtcggc ggcagcattg ctcattttgt gcccgctgtt gacgtccacc acatgcagcg
     1561 cttcggtgga ttcgatgact aagtacgcac cgctgctgag agtggccgtt tttccaaacg
     1621 aagctttgat ctggcggtcc acgctgaagg tctcaaaaat cgggcgactg cctttgtgca
     1681 aagacagtat cttgatctga tcgggcgcta tggtagctaa gtagttcttg atattctgaa
     1741 acaactcctt gtcgttcacg acgatgcggt tgaaggagtc tgtcagcagg tcgcgcagga
     1801 tactggaggt tttgtccaat tcgctgagca atttggcggg cggcgtagcc ttgtacatct
     1861 gttggtagat gtcttcccat ttgctcatca tgaaggccag ctcttcgtgc aattctgccg
     1921 tttttttacc ctcggcagcg gtgcgtacaa tgacgccgaa gtttttgggc cggatgcttt
     1981 ccaccaaatt ttgcaggcgt ttgcgctcct cggcatttcc tattttttta gaaactgcca
     2041 ccacttcatt gaacggggtg agcacgagat agcgaccggg aatggtaacc tcgcaactca
     2101 gacgcggacc tttggtagag atgggttctt tgagaatttg caccagaata ttctggcgct
     2161 tttccaacac ctgatccact ttgccggtct tgacgatctc cggctccaat ttgaagttat
     2221 cgagcttgtg ggtggaaatg tttcccgcag tgacgccggc ggaatacttg aggagcgagc
     2281 gcagtttggg gcctaagtct gtataatgca aaaacgcatc ttttttgtgt ccgatgtcca
     2341 caaaagcagc attgaggccc ggcatgagcc gttgcacttt gccgaggaat acatccccga
     2401 cactaaagtt gctgtttgtt ttttggtagt gcagctctac caatctgccg ttctccatca
     2461 aggcaatttc aacctcggta ggagtggcgt taatgatcat ttctttttcc acggggcaat
     2521 acatttacgg gcgccgcaaa gcggtgcgaa ttggcatgat gcagtcatgc aatatatccg
     2581 tattgccggt gtgtgaatgt gtttctgaaa attcgtaccc ccaaacttac aaggttgcaa
     2641 gtccgacgat ttgccttgag tacattacaa attgaaacat gtacctcaag gggccgtatt
     2701 cagaattagg aacacaatct gaacgtcttg ctattacata gaatgtaagc aggcattcag
     2761 atcgtgttca tttgctttag gcgttgcagt tctctcctta gaaaacagcc gtacagcaaa
     2821 acagacttcc gggaagaagg attcatcatg gggagatcac cgctttcgga tgtgaataga
     2881 tgtatccgat tgcagggaaa acggtctgca tcaatatgca aattcccacc acctcgatgc
     2941 cgatgatgaa atcttacttc ttcttcttgt gacggttctt gcgaagtctc tttttacgtt
     3001 tgtgcgttgc aatcttgtgc ctcttacgtt tttttccaca aggcatagtg aatctctttt
     3061 tttgttttac aaaaatggtt gttgtcaaag tccttagccc cattgggata aggaaggaat
     3121 cattcgttga gttggccgca ggcttttgca aattgctcag ccttggcggc gtcgccggcc
     3181 ccattccagg catctgccaa caagcaggta gctgtaaggt tgccgggttc gatttcgagt
     3241 acgcgttcca gacgttggac tgctttatcg aactgacctg tttggatagc taatcggccc
     3301 aaattattga gcaccgacac gttttccggg tactgtcggt tcaactccag cagcatggtg
     3361 atacctcgca tgacatcctc tttgggagga ttttcggcgt gtacgaccgc cagattgacc
     3421 cggtgagcaa cattttccgg attaacagaa atggcgctct caaaagcagc gacagctctt
     3481 tgagtgcaat aggtacgcac cttctcgtct tcgctgcgct ggaggcatat cgcataagtg
     3541 gtaccggctt tcgaccaagc atcctctgtt tgcagccttt gtgccaccaa ttgtgcataa
     3601 tgtcccgata tggcaggctc gccggcattg aaccaagccc ccgccaaacg catcagcgta
     3661 gaagtgtacg cagaggaatc gtcttgaacc aattccaact cgtgctctat gctgccgatt
     3721 tcgtttttgc tattggcttg caaccggccc tgcgcttctt tcatcagcac gtttacatca
     3781 gtgctttcgg cactgaaaac ccttgtcttt tcaagggatt gttggtcttt cgactgggtt
     3841 tcacatccaa aatacagcgc aaagaacagt attgtggccg aaaccaacgc cgtaatttgt
     3901 cttagtcatg tggatgtgtt atgacctcag cgaaatatta atcttcttct tcttccggca
     3961 tttcggtgac gttgttcttc acctgatcta caaacacttt ggccggtttg aaggaaggaa
     4021 taaagtgttc tggaatttca atggcttcgt tgcgcttgat gtgacgaccg attttcttgg
     4081 cacgtctctt aataacgaag ctaccgaaac cgcgaatgta aacgttttcg cctcctgcaa
     4141 gggagccttt tacttctttg aagaaagttt ccagtgcgac cagcacatca actttggcta
     4201 ctcctgtctt ttcggagatg gcatttacca gatcggcctt tctcatcgtt ataaattggt
     4261 ttataataag ttatgaaaag aaaaccaccg ctttttcaag cgagacgcaa atgtcggaat
     4321 atttcccgga ttccggcaag aaaaaatgag gttaattttc agcactttgt gtttaatttt
     4381 cctcaaagaa ccgcaaaacc tatgaattgc aaagatgttg aatgataaaa atcaagtttt
     4441 tattggttat ttcaaatatg cgacttactt ttgaaacaaa ttttgttgaa cttatgaaaa
     4501 aacaagctac gcaccgcatg tataatgtgc ccgatgccga gctgtatgta caatgtatgg
     4561 agcgcatccg atacgcttac cgagacatag accgctttgt gccgtactct tatgacctcg
     4621 aacggcttcg ggcttttggg gcgctgtgcg aacgtttcaa cgccatgccc gacgatgacg
     4681 aaatggtggg cgaccagatg ctgatgaccg aaaagaagga cgatgccgcc gagcgactca
     4741 aaacagccat ccgtagcctc atgacacggg tggaaatcaa attcaacaac agaagcgggc
     4801 gctatcggaa gttcggcacg gctaaaatgg gcgatatgtc cgatgctcaa ttgttgttct
     4861 gtggaagaag agtagctcgg gtagcccggc aacaaatgga ctttttggca gataccggca
     4921 tgaatgaaac catcattcaa cgcgtgacgg atgcgtgccg ggactttgaa acagctctga
     4981 acatccagca agaccgtatt gccgaccggg acatcgccgt tgaaaaccgc accgaacaag
     5041 gcaataaaat gtatcaggaa ttggtagtgc tttgcaatat cggcaaagac atctgggctg
     5101 ataccgaccc agtcaaatac gagaactact gtatctacga gagcaacaat caccaaaaga
     5161 ttgcccgtaa ggagagggaa aaatccgccg tggcgtctaa ggaatgatgc caatcaaggc
     5221 ttccattcat tgtttgccct gtttacatcg gccattctgc cggtagggtc tatcaccacc
     5281 ttggctatgt ccttgtgcct cacaggtaca gaccattgat aataaggctc aacccagggc
     5341 caagcttctg ttttttcaaa accatcccac cctgaatctg agggttttcc gccccgcatc
     5401 atacacagag ggatgtgata gcgcatcgtt tggccgtcgt tgagcgtgat ttccacgtcc
     5461 acaggcatag gcatggcgcc cttccggtgc aggcgaatga tggaggactt gctgcggtcg
     5521 gcttctacgg cggctactat ataatctatg gtgtgcgtag aatacaccca atagtcgaga
     5581 taccaatcca attcgaggcc ggaagcgcgt tccatgaccc ggatgaaatc tttatcatcg
     5641 gggtgcttga atttccatgt atcgaaataa cggagcaagc cggcattgaa tgcctccagc
     5701 ccgatgaggt aggacaactg cgagaggaag accgaacctt tattgtaact ggccacgcca
     5761 taggccgtat tggtttggaa atgatccgaa ggcgtgctga gcgcttcttc ccaatcgctg
     5821 agggcgatat ttacatagcc ctggtaatca ggtttgtgcg gcatatttga tacgctcctt
     5881 cccggcaaca gcttctctcc ggccagatga ttcatcactt ccgaagatgc aaagtttaca
     5941 aaaccctcat ccatccatgc gtaacgcgcc tcgttggtgc tcagtaccat cggataccag
     6001 cagtgcatca gttcgtgtac cgatacgccc accaagctgt tgaggggtct gtttccggtg
     6061 atgagcgtag ccataggata ttccatgccg ccgtcgccgc cctgaatgaa ggcgtactca
     6121 gggtaaggat attgaccgaa gcgcgcattg atgtaatcga acgcccgatc catgatggcc
     6181 ggcagccggc tccatgcctg agttgcttcg ccgggctggt agaaaaagcg cagcaccgta
     6241 ccgtccgctc ttgtgagggc agtgtgttca taatccggat cggcggccca tacgaaatcg
     6301 ttgacgtttt cggctttgaa atgccattgc aagcggcggg gaaggttctt gggggcttcc
     6361 tccggtcggc aatagccatg cccgatttcg gtgggatttt gcagtatgcc ggtagcagct
     6421 actnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcacattga aatctccaaa cacgccatag
     6481 aactccctgc ctatgtaatc gtcggcatgc cagccgtgac ggtcgtaagc acagactttg
     6541 ggataccatt gcgccataga gtaactgata ccctcggcat tgttgcgtcc attgcggcgt
     6601 atttgcacgg gtacctgcga ttcaaaggtc atatccaact tcactgtttc gccgggcata
     6661 agaggctcat tcagcgatac ttccagaata gtgccacgaa cggtccagtt gcgcagttcc
     6721 tgatcgttct gcctcatttt caacacttta gtaaagccga tttcattggg tttgagctta
     6781 gaannnnnnn nnnnnnnngg agtcggaatc ggatatattg cgggaacgca tatccatcat
     6841 gctccccggc tgaaaagcat tgagatagag atgatagaag acgcggtgca gtgtatcggg
     6901 cgattggtta tggtaatgca agacctgctc cccgtcgaag cgatgttttt tggtatcgaa
     6961 cccaatgctc atgtcgtagt tgacgcgttg ttgccaatag cctgtacctg gttgtccgct
     7021 tgccatgagg gcgctgtaaa gtagggccgt cagtgagaca gctctgatca tcttggtcat
     7081 tcttggaaat gttgtggtcg ttaaatattc ggagtatcgc gctgttttct aagcactgct
     7141 ctgagcatga taatgaacag aatggccagt acggcaaagg gcagccaact gccaatctgg
     7201 tgtccgatct gatagcctgc cgaggtggta tctactcctt ccattgtgat gctgaatgat
     7261 gtggcaataa taagtcattt aatcagcaaa agttgagtaa agggtccatt cctttcgtat
     7321 tcttccccga aaagtcggtt cttgtactta ttttcatcgg cgtttgtccc ggcatagtcg
     7381 aataaaagta gatatcccaa cagagtgcag gcatttttgt gagcaaaaag cagcaattgg
     7441 acattcaacg caaagcggcc gactgggccg gagaactcat taaagatcgt tttatggaaa
     7501 acaacattca ttccgagcgc aacaagcacg ccgagaccat cattcgcaac cacattttgt
     7561 ggtctatggg cgccagtttt ctcatcccta tacctgtggc cgacattttt gccgtcagcg
     7621 cgcttcagct tgacatgatt cggcaattgt gcagggttta caatgtcgac tttgccgaaa
     7681 cacagggtaa agctgttgtt acatccctca catcgagcac tttagcgaaa gtaggagcga
     7741 ggagccttat caaaatcatt cccggtatcg ggcaattggt aggaggcgtg gctacttctg
     7801 ttttcaacgg cgcttccact tacgcgctgg gcgaggtgtt caaaaaacac ttcgagtcgg
     7861 gaggaaccat attggatttt gatacggacc ggctcaaaaa attctatcag gagaagtttg
     7921 aaaaaggcaa aaaagtggcc gaacaattga agaaggatca ggaagccaaa aaggccgccg
     7981 agaatgctgc acctgccgcc gcttcaggtc cgtctgctcc ttcgcctgcc gccgcccagc
     8041 catctgccgc ggatcgcctt cgggaattgg ccgagcttaa aaaccaggga attatctccg
     8101 aagaagagtt tgaacaaatg aaaaagcgcg ttatagagag tttttaagcg ctcagaccat
     8161 atcaaattag tctaaagaat ccccgacgga tagtacatcc atcggggatt tctattttaa
     8221 atcattgatt ctatgcagat tatatcaatt cagcacagac caaagcattt tttcatcatc
     8281 gtgttttaga aatgaaaaat gtcacaaaaa aatagcatta taacaaatta aatatttata
     8341 aatgatatta ttacaaataa gttttaacat taatgttaat aatattttta gtcacgtttg
     8401 ctcattttta gtataaaata aaatattaaa actcttttgt tttgcagcat tcttatcatt
     8461 aagtatttag tgttgttcat agtgtttgtt tttgcttccc ctatcccccc cgatagggga
     8521 ttttttttgc caacttaatc ctgttccacc ttgtttcgtc tccgaatttc atctgataat
     8581 aaacgcggaa tcttatgtta ttggacttac atcacctccg ggaagagtat gccgaacgca
     8641 agctggatgc ttcggaggct cacacagacc ccttcattca attgcagcaa tggctcaatg
     8701 atgcgcttat ggcagagctg cccgaaccca atgccatgac cttggccaca tgtacaaagg
     8761 agggtatccc ttccgccaga gtggtgctgc taaaaggcgt cgatcgctcc gggctggatt
     8821 tttacaccaa ttaccacagt cgcaaagggc gtgaaataga tgaaaacccg catgttgctg
     8881 ttactttttt gt
//
LOCUS       DAORLF010000175         8866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4269_length_8866_cov_2.354103, whole genome shotgun sequence.
ACCESSION   DAORLF010000175 DAORLF010000000
VERSION     DAORLF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_8866_cov_2.354103"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>510)
                     /locus_tag="PK198_14090"
     CDS             complement(<1..>510)
                     /locus_tag="PK198_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39915.1"
                     /translation="YLFNIPVRTTPSSFSIINAGSGFDRLFPDTLTNAGTARNFGLEA
                     TVERYFTKGYYFLFTGSLFDSKYRGSDNVLRHTSFNGRYAFNALIAKEFTFSKGSALN
                     IGGKLTMAGGRRYGQVDKAASDAVQEIIYIDATVNTLQFKDYLRADLKLSYRWNRPKA
                     THEFSIDIV"
     assembly_gap    511..520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<521..>1918)
                     /locus_tag="PK198_14095"
     CDS             complement(<521..>1918)
                     /locus_tag="PK198_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39916.1"
                     /translation="PVSARQFSVEETDRYAGSRGDPARMASNFAGVQGADDSRNDIVI
                     RGNSPQGVVWRFEGINIPNPNHFAIPGTTGGPLSIINNKYLDNSDFFTGAFPAQYGNG
                     IAGVFDLRMRNGNNRRHELSAQFGILGTELSAEGPLSREAKSSYLLSYRYSTLRLFDF
                     LGIRLGTDAVPQYQDGAFRFSWPFKNGGQLALWGIGGASRIVVVNSELEAPAENIDLY
                     AESDRDQVTTSRMGVVGLTFSQPAGVSSFFKASLAVSNQRVSFEHEKIIRHIENERYV
                     LDSLPRVMDARLGESKISANFGYHHKFNKKTNMVAGLNSDYWIFNYLDSARSILLFGP
                     DVQVHPWRLRWDTRNQGALMLQPFVQMQHRLNERLSLSAGISSLWFGLNKNSFSPIEP
                     RLGLSYEMPARQRLSFGYGLHSQTLPPYLYFYGKEMRNGDPQEHNLDLGLIKSHHLVA
                     GYERFVGASTRLKVET"
     assembly_gap    1919..1928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1929..2315)
                     /locus_tag="PK198_14100"
     CDS             complement(<1929..2315)
                     /locus_tag="PK198_14100"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39917.1"
                     /translation="MRYLYILLAALLSSTLLPAQTPHTQTIRGIVTDKITKTPLIGVN
                     VLLLNTGDELNGTVTDYDGSYLLEKVPMGRRSIEFSYIGYNKISLDNIVVSSGREVVL
                     NVEMEESVTELKTVEVAARRSGDTRNE"
     gene            complement(2495..4000)
                     /locus_tag="PK198_14105"
     CDS             complement(2495..4000)
                     /locus_tag="PK198_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF39918.1"
                     /translation="MKEKPNLSFWQIWNMSFGFLGIQYGFGLQQANMSPIYRYLGAEE
                     ADIPGLWLAGPLTGLLLQPIIGAMSDKSWSPRWGRRKPYVFFGAIAGSIAMIMMPNST
                     SVFMAACLMWLLDAGLNSSMEPFRAFVGDILNTKQRPTGFSVQSFMVGFGQTLANLMP
                     FILPLVGISMALTQDQTDTANGIPNSVRYPFYIGAAAIILTVLWTMYTTKEYPPENDD
                     YKKRHVFTAEQNKSILKWRLAMSAGFAVLGGIFAWQASGVLYGLLWASGIFAASYALL
                     LLPVFKEILAPLSEMPDVMKQLWWVKFFTWYGLPLMWQYLTLAAAKYAFDAPNADSPG
                     FEEGKNWGNLCFAMFSITCFVISIFMPRISKAIGSRRATHALFLSIGAMGFFLTLTSN
                     DKYIYLAGMSLVGLAWGSIMSMPYLMLANAVSKERMGVYMGIFNGFICIPQFINMATV
                     PLYYKTLLGNDPRNALVLAGICLLLAGASNFLIKERVNDEADADAVPAGGH"
     gene            complement(4138..4767)
                     /gene="rsmG"
                     /locus_tag="PK198_14110"
     CDS             complement(4138..4767)
                     /gene="rsmG"
                     /locus_tag="PK198_14110"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRF39919.1"
                     /translation="MSSLLLPYFPDLDERQQAAFAQLQPLYTEWNEKINVISRKDIAE
                     LYERHILHSLAIAKVLRFLPGSAVLDLGTGGGIPGIPLAIMFPETKFLLADSIGKKIK
                     VVQEIAETLGLSNVTARHTRAEEVKERFDFVITRGVAPLDKLLIWSRPLLKKKHLHAI
                     PNGLIALKGGDLRPEFDTLHRNEYVETWRIADFFKEPFFEEKYVVYVQG"
     gene            complement(4764..5459)
                     /locus_tag="PK198_14115"
     CDS             complement(4764..5459)
                     /locus_tag="PK198_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRF39920.1"
                     /translation="MNQEESKVQDFRIGDQVLYKNNQLIAFNKPVMLPVQPDKTGDKS
                     LLELAEMYAHSKLHLVHRIDRPAGGVVLFAKNTTALAALHEQFRLREVEKTYLAVVQQ
                     LPPEPSGQLRHYLVKNGRTNRSSLTETAVPGAQESVLDYRLLGSSERYHLLEIKLHTG
                     RHHQIRAQLAAIGCPIKGDVKYGFRRSNPGGGIHLHAWKLSFRHPVSNEAVTITAPIP
                     TDDPVWDALKPAE"
     gene            complement(5461..5637)
                     /locus_tag="PK198_14120"
     CDS             complement(5461..5637)
                     /locus_tag="PK198_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39921.1"
                     /translation="MKFLFTLALLYLAYRFFVVRPAIAAAAKRYRDTLRSDPPPAATR
                     KKRDDEYIDYEEVD"
     gene            complement(5700..6530)
                     /locus_tag="PK198_14125"
     CDS             complement(5700..6530)
                     /locus_tag="PK198_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF39922.1"
                     /translation="MVSASAMKWITYSVVILTFLWILGNIFTGPLQHWFIFRPKKLPQ
                     NHKYQFEAPFEEMFIETPRGGRLNALWFKAAGNEPAKGLVLYYHGNAGSLERWGHLHH
                     FFARHGYDFLVYDYRGYGKSTGRRNQKMMYADALEVYRFARKHYAPDQILLYGRSLGS
                     VFASRVAAEQSARILVLETPFYSMTDLFYTYYPFLPKAFYLRYRFSNARSLRQVQIPV
                     VVFHGTEDLVVPYRSSERLKKVLKPGDQFHTIPGGSHNNLLFYDIYNSKMEDILSGAE
                     "
     gene            complement(6618..7055)
                     /locus_tag="PK198_14130"
     CDS             complement(6618..7055)
                     /locus_tag="PK198_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRF39923.1"
                     /translation="MHLSYEESKKHVVKGLILLAVITLIEVFLSLIGKGDLLSGLSAA
                     APKVTLLVTGLLIIVLSIYKAYYIIGDFMHMKYEVRGLAMSVLLPTLLLVWAIIAFLQ
                     EGGSWGARREWVREKDKAPAEQTAKPKQQGMLPGSEDGWYRMF"
     gene            complement(7091..8110)
                     /locus_tag="PK198_14135"
     CDS             complement(7091..8110)
                     /locus_tag="PK198_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF39924.1"
                     /translation="MASKTDHLEREHEVHDGKEDWSGGKEPMNVSYGKLMMWYFLLSD
                     AFTFAGLLIAYGALRFSSPSWPSPDFVFSTYPFGKEGSHLPLIFVTIMSFVLIISSVT
                     MVRAVQEGKRENKNGVVFWLLMTVLGGASFLGCQAWEWTNLIVKENMSVTTNPFSVHN
                     AGGVYLNADGSEAKKEDGSPDKFESGDGYLIHRATILKDGDPRGQASHEAGGHGDEAG
                     GHHHEVFVYESQLPLDEAGQYIDERGYIHKKFKVTEGDNAGKVMTEEFGPKAFGALFF
                     FITGFHGFHVFSGVAFLLIIAINAASGLYVARKNGYEMVEKIGLYWHFVDLVWVFVFL
                     VFYLL"
     gene            complement(8133..>8497)
                     /locus_tag="PK198_14140"
     CDS             complement(8133..>8497)
                     /locus_tag="PK198_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF39925.1"
                     /translation="FAYTSFKNGREQAYRWLLSAAFVLGFAFLALQYQGWVALKEMGV
                     AINTNPSGSFVYVISGIHAAHILGGIGALLVALLHAWSLPYKVTQQRKLRFEMTLTYW
                     HFVDFLWVYLFFFFISQS"
     assembly_gap    8498..8522
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8523..8726)
                     /locus_tag="PK198_14145"
     CDS             complement(<8523..8726)
                     /locus_tag="PK198_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase subunit III"
                     /protein_id="HRF39926.1"
                     /translation="MNAGTAPMEYGSKNKIHPKKFALWVACAGITMMFASLTSSYLVR
                     RAAGNWLEFPLPVVFWYSTVVIVA"
BASE COUNT         2175 a         2306 c         2365 g         1975 t
ORIGIN      
        1 tcacgatgtc aatgctgaat tcgtgggtgg ctttgggcct gttccagcgg taagagagct
       61 tgaggtcggc acgcaggtag tctttgaatt gcagcgtgtt cactgtggcg tctatataaa
      121 tgatttcttg cacggcatct gaggcggcct tgtccacctg tccgtagcgt cgtccgccgg
      181 ccatcgtcag tttgccgccg atgttgagcg ccgagccttt agaaaaggtg aattctttgg
      241 caatcaaggc attgaaagca tatcggccat taaaggaggt atgccgcaac acgttgtcgc
      301 tgccccggta cttgctgtcg aacaaagacc ccgtgaacag aaaataatac cccttggtaa
      361 aatagcgctc taccgttgcc tccaggccga aattacgggc tgtgccggcg ttcgtgagcg
      421 tatcggggaa aagccggtcg aagccggaac ctgcattgat gatgctgaag gaagagggcg
      481 ttgttcgcac cggaatgttg aataaatact nnnnnnnnnn cgtctccact ttgaggcggg
      541 tggaagcgcc cacaaaacgt tcataacctg ccaccaagtg atggcttttg atgaggccga
      601 ggtcaaggtt gtgttcctgc gggtcgccgt tgcgcatctc tttgccgtag aaatagaggt
      661 agggcggcag ggtttgggag tgcagcccgt agccgaaact aaggcgttgt cgggcgggca
      721 tttcgtagct gagtcccagg cggggttcta ttggcgagaa gctgttcttg ttcagtccga
      781 accaaagact actgatgccc gcgctgaggg agagccgttc attgaggcgg tgctgcattt
      841 gtacaaaagg ctggagcatg agtgcgcctt ggttgcgggt gtcccaacgc aagcgccagg
      901 ggtgaacctg cacatcgggg ccgaagagga ggatgctgcg ggcgctgtcc aaataattaa
      961 aaatccagta gtcggaatta aggccggcca ccatattggt ctttttgttg aacttgtgat
     1021 ggtagccgaa attggccgaa attttgcttt cccccagacg ggcatccatg acgcggggca
     1081 agctgtcgag cacatagcgc tcattttcta tgtggcgaat gattttttcg tgctcaaacg
     1141 aaacccgctg attcgatacg gccagcgatg ctttgaaaaa cgaggaaacg cccgcaggct
     1201 gtgaaaaagt aagcccgacc acgcccatgc gcgaggtagt gacttgatcg cggtcgcttt
     1261 cggcatacaa gtcaatgttt tcagcaggcg cctccaattc gctgttcacc accacaatcc
     1321 tgctggcgcc gccgatgccc cagagtgcga gctggccgcc gtttttgaat ggccacgaaa
     1381 acctgaatgc gccgtcctga tactgcggca cggcgtcggt gccgagccgg atgccgagaa
     1441 aatcgaagag ccgcagggtg gaatagcgat aggagagcaa gtacgaggat ttggcttccc
     1501 gcgaaagcgg tccctctgcg ctgagttcgg tgccgagaat gccgaattgc gccgacagtt
     1561 cgtggcggcg gttgttgccg ttgcgcatac ggaggtcgaa aacgccggca atgccgttgc
     1621 cgtactgtgc cgggaacgcg ccggtgaaga agtctgaatt gtccaaatac ttattgttga
     1681 tgatactcag cgggccgccg gtggtgccgg gaatggcgaa gtggttgggg ttgggaatat
     1741 tgatgccctc gaaacgccac acgacgccct gtgggctgtt gccccgaatc acgatgtcgt
     1801 tgcggctgtc gtcggcgcct tgcacgcctg caaaattgga agccatgcgg gccgggtcgc
     1861 cgcggctgcc tgcatagcgg tcggtttcct ccacgctgaa ctgccgggcg ctcaccggnn
     1921 nnnnnnnntt cattgcgggt atcgccggaa cgacgggcgg ccacctccac agtttttagt
     1981 tcggtgacag attcctccat ttccacattg aggacgacct ccctgccgga acttaccacg
     2041 atgttgtcga gcgatatttt attgtagccg atgtagctga attcgatgga gcggcggccc
     2101 atgggcactt tttccagcag ataggagccg tcgtaatccg tgacggttcc attcagttcg
     2161 tctccggtgt tgagcagcag tacattgacg ccgataaggg gcgttttggt gattttatcg
     2221 gtaacgatac cgcgaatggt ttgggtgtga ggtgtttgag ccggcagcag ggtggaactc
     2281 agcagtgctg ccagtaagat ataaaggtat ctcatggctg cgtggcgttg gttaggaatg
     2341 gaggtaaggt acacctttag cgcgctgccg gacaatgatt tttgtcagag ataaagagag
     2401 acgtttgtac atagcaaagg ggtgattccg gcaagaccac ggccccggag tcaccccttt
     2461 atgatacata aaatttaacc ctttccccta agcgtcaatg cccgcctgcc ggaaccgcat
     2521 ctgcatctgc ctcgtcgttg acgcgctcct taatcaggaa attggaggcg cctgccagca
     2581 gcaaacagat acccgccagt accagagcgt tgcgcgggtc gttgcccagc agcgttttat
     2641 agtacagcgg cacagtggcc atattgatga attgcgggat gcaaatgaag ccgttgaaaa
     2701 tacccatgta cacgcccatc cgctctttcg acacggcgtt ggccagcatc aggtacggca
     2761 tagacatgat ggaaccccaa gccaaaccca ccaagctcat gcccgccaga tagatgtatt
     2821 tgtcgttgga tgtcagcgtg aggaaaaagc ccatagcgcc gatgctcaaa aacagggcgt
     2881 gtgtggcccg gcggctgccg atggctttgg aaatgcgagg catgaagatg gagataacaa
     2941 agcaagtgat gctgaacatg gcaaaacaga ggttgcccca gttcttgccc tcttcgaaac
     3001 cgggcgaatc ggcattgggc gcgtcaaagg cgtatttggc ggcagccagc gtgaggtatt
     3061 gccacatcag cggcagacca taccaggtaa agaacttcac ccaccacaat tgcttcatca
     3121 catcgggcat ttcggaaagc ggggcaagga tttctttaaa aacaggcaac agcagcagtg
     3181 cgtaagatgc tgcaaaaata ccggacgccc aaagtaatcc gtataaaacg ccggaggcct
     3241 gccatgcaaa aatgcccccg agaacagcga accccgccga catggccagc cgccacttga
     3301 ggatgctctt gttctgctcg gcagtgaaga cgtgcctttt cttgtagtcg tcgttttccg
     3361 gcgggtactc tttggtggtg tacatagtcc acagcaccgt aagaataatg gcggcagcgc
     3421 ctatatagaa cggatagcgc accgagttgg ggataccgtt ggccgtgtcc gtctgatctt
     3481 gtgtgagggc catagaaata cccaccaaag gcaaaatgaa cggcatgagg ttggccaagg
     3541 tttgcccgaa tcccaccata aacgactgca ccgagaagcc tgtcgggcgc tgtttggtat
     3601 tgagaatgtc gcccacaaaa gcgcggaacg gctccatcga agagtttagg cccgcatcca
     3661 agagccacat caggcaggcc gccatgaaca ccgaggtgga attgggcatc ataatcatgg
     3721 caatactgcc ggctatggcg ccaaagaaaa cataaggctt gcggcggccc cagcgcggcg
     3781 accagctctt gtcggacata gcgccgatga tgggttgcag caacaaacct gtgagcggcc
     3841 cggccagcca aagccccgga atatcagcct cttcggcgcc cagatagcga taaatgggac
     3901 tcatgttggc ttgctgaagg ccaaagccgt attggatgcc gaggaatccg aaactcatgt
     3961 tccagatttg ccaaaagctc agattaggtt tctctttcac agatagttgg ttttgatgca
     4021 gcgaaaatat aaaagcctgc aacaaaccgg ggcaaaataa actatcaatt ttccgggaat
     4081 gtacaatttg tttctacaac accctactca cccctcacct actcacccct atccacctca
     4141 cccctgcaca tacaccacat acttctcttc aaaaaacggc tctttgaaaa aatcggcaat
     4201 gcgccaggtt tccacatact cattgcgatg cagcgtgtcg aattcggggc gcagatcgcc
     4261 gcctttgagg gcaatcagtc cattggggat cgcgtgcagg tgcttttttt tgagcaaagg
     4321 ccggctccag atcaggagct tgtccaatgg cgccacgccg cgcgtgatga caaaatcgaa
     4381 gcgctctttc acctcttcgg cgcgggtatg ccgggccgtc acgttggaaa gccccagcgt
     4441 ttccgcaatt tcctgcacta cttttatctt tttgccgatg ctgtcggcca gcaggaactt
     4501 ggtctctgga aacataatgg ccagcggaat gccgggaata ccgccgccgg tacccagatc
     4561 gagtacagca gagccgggca gaaagcgcag cactttggca atggccaagc tgtgcaggat
     4621 gtggcgctcg taaagttcgg caatgtcttt cctggaaatg acattgatct tctcgttcca
     4681 ctctgtgtac aggggctgca attgcgcgaa agcggcctgt tggcgctcgt ctaagtcggg
     4741 gaaatatgga aggaggaggc tgctcattct gccggtttta aagcatccca caccgggtca
     4801 tccgtcggaa tgggcgcagt aatggtaacc gcttcgttcg atacagggtg ccgaaacgac
     4861 aatttccagg cgtgcaggtg gatgccgccg ccgggattgc ttcggcgaaa gccgtatttc
     4921 acatcgcctt tgatggggca gccgatggcg gccaattgtg cgcgaatctg gtggtggcgg
     4981 cccgtgtgca acttgatttc gagcaggtgg tagcgctcgc tgctgcccaa caggcggtag
     5041 tccaacaccg attcctgtgc ccccggaacg gcagtctcgg tgagggaact gcgattggtg
     5101 cgcccgtttt tgacgaggta gtggcgcagt tggccgcttg gctccggcgg cagttgttgc
     5161 accacggcga ggtaggtttt ttccacctcg cggaggcgaa actgctcgtg cagggcagcc
     5221 aatgcggtcg tatttttggc aaacaacacc actccgccgg cgggccggtc tatgcggtgt
     5281 accaaatgca gtttggagtg cgcgtacatt tccgccagtt ccagcaatga cttatcgccg
     5341 gttttgtcgg gttgcacggg cagcatcacc ggcttgttga aggcgatgag ctggttgttt
     5401 ttgtacaata cctgatcgcc gatgcgaaag tcttgtacct tggattcttc ctgattcata
     5461 tcaatcaacc tcttcgtaat caatgtattc gtcgtctctt ttttttcgag tagcggcagg
     5521 tggcgggtca gaccgcaaag tatcccgata gcgcttggcc gccgccgcga tggccggccg
     5581 cacgacgaaa aaccggtaag ccaaatacag cagtgccaga gtgaataaaa atttcatact
     5641 gaaaactttg gtacaaaggt agcgaatgtg ggctgaatgg acaaatggag cgcctcccct
     5701 tactccgcgc ctgaaagaat gtcttccatc ttgctgttgt agatatcgta gaacagcaga
     5761 ttgttgtggc tgccgccggg tatagtgtga aattggtcgc ctggtttgag tacttttttc
     5821 agacgttccg aacttcggta aggcaccacc aaatcttctg tgccgtgaaa aacgaccacc
     5881 ggaatctgta cctgtcgcaa cgaacgcgca ttcgaaaaac gataacgcag gtaaaaagct
     5941 ttgggcagga aagggtaata ggtataaaac aaatcggtca tactgtaaaa cggcgtctcc
     6001 aatacgagta tgcgggcgga ttgctcagcg gccactcgcg acgcgaacac ggaacccagg
     6061 gaacgcccat acagcaaaat ctgatcgggc gcatagtgct tgcgggcaaa gcgatacact
     6121 tccaaggcat cggcatacat cattttttga ttgcggcggc cggtgctttt gccgtagcct
     6181 cgataatcgt acaccaaaaa gtcatacccg tgacgggcaa aaaaatggtg caaatgcccc
     6241 caacgctcca agctccccgc attgccatga taatagagta ccagaccttt ggctggctcg
     6301 ttgcccgcag ctttgaacca caaggcattg agcctgccgc cgcgcggcgt ttcgatgaac
     6361 atttcctcga agggcgcctc aaattgatac ttgtgatttt ggggtagttt tttgggcctg
     6421 aaaataaacc aatgctgcaa tggcccggta aagatattgc cgagtatcca caggaaggtc
     6481 agaatcacca ccgagtaggt tatccatttc atggcggaag ccgaaaccat aggcattatt
     6541 aggctgctaa aacggcagag gccttgcgtt cattgccgca aagcctctgc ctgagtatga
     6601 aaaaccgctc ccggaaatca aaacatccga taccagccgt cctcgctgcc aggcaacata
     6661 ccctgctgct taggcttggc ggtttgctct gccggcgctt tgtctttttc gcgcacccat
     6721 tcacggcgcg caccccaaga gccgccttcc tgcaagaatg caataatggc ccacaccaac
     6781 agcagcgtcg gcagcaatac cgacatggcc agaccgcgca cctcgtactt catgtgcatg
     6841 aagtcaccga tgatgtagta cgccttataa atagagagta caatgatgag caagccggtg
     6901 acaagcaagg ttaccttagg cgctgccgcc gataaaccag acaacaaatc gcccttgccg
     6961 atcaatgaaa gaaacacttc aatcaaagtg atgacggcca ataggatcaa acctttgaca
     7021 acgtgctttt tggattcttc gtagctgaga tgcatcgtat gatgtatttt tcagagtgtt
     7081 caaaaatgaa ttaaagcagg tagaaaacca agaatacaaa tacccacacc aagtccacaa
     7141 agtgccagta caagccgatt ttttctacca tttcatagcc gtttttacgg gctacataca
     7201 aaccggaggc tgcattgatc gcaatgatga gcaaaaatgc aacgccggaa aaaacgtgga
     7261 atccgtggaa tccggtgata aagaagaaca aagcgccaaa tgccttcggg ccaaattctt
     7321 ccgtcatcac tttgccggcg ttgtcgcctt ccgtcacttt gaattttttg tgaatatagc
     7381 cgcgctcatc aatgtattgg cctgcctcat ctaaaggcaa ctggctttca taaacgaata
     7441 cctcgtgatg gtggccgcct gcctcatcgc catggcctcc agcttcgtga gatgcctgcc
     7501 cgcgcgggtc gccgtctttc aatatcgtag cccggtgaat gaggtagcca tctccggatt
     7561 caaatttatc aggcgagcca tcttcctttt tagcttccga accgtccgcg ttcaaataca
     7621 ctccacccgc attgtgtaca ctgaagggat tcgtcgttac cgacatgttt tccttcacaa
     7681 tgaggttggt ccattcccac gcctgacaac ccaagaagga agcccctccc aataccgtca
     7741 tcaagagcca gaaaacaacg ccgtttttgt tttcgcgttt accctcctgc acggcgcgca
     7801 ccatcgtaac agagctgatg atgagcacaa atgacataat ggtaacgaaa ataagcggca
     7861 aatgcgagcc ttcctttcca aaaggatagg tagagaatac gaagtcgggg ctgggccagc
     7921 ttgggctgct gaatcgcaga gcgccatacg ctatgagcag gccggcgaaa gtaaaagcat
     7981 ccgacaacag gaagtaccac atcatcagct tgccatagct gacattcata ggttccttgc
     8041 cacccgacca gtcttccttg ccgtcgtgaa cttcgtgttc ccgttcgaga tgatccgttt
     8101 ttgaagccat cggttatccg atttttgcgt gtttacgatt gtgaaataaa aaagaaaaac
     8161 aaatataccc agaggaaatc aacaaaatgc caataggtga gcgtcatttc aaaacgcagt
     8221 ttgcgttgtt gcgtcacttt gtaaggcagg ctccaggcat gcagcaatgc caccaacaga
     8281 gcgccgattc ctcccaatat atgagcggca tgaatgccgg aaataacata tacaaatgat
     8341 cccgaaggat tggtattaat ggctaccccc atttctttca gggctaccca accctgatat
     8401 tggagcgcca aaaaagcgaa ccccaacaca aaagccgcac tcaacaacca gcgataagcc
     8461 tgttcgcggc cgtttttgaa agaagtgtaa gcaaaatnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnggccacaa tgacaaccgt gctgtaccaa aacaccaccg gaagcggaaa ctccagccag
     8581 ttgcccgctg cccgacgaac taaataggaa ctcgtcaaag aggcaaacat catggtaata
     8641 ccggcacaag ctacccaaag tgcaaatttc ttgggatgaa tcttgttttt gctgccgtat
     8701 tccataggtg cggttcctgc gttcataaaa aaattaaatt ttatcggcga ataacgcaat
     8761 gagcgtcaac ggaatgtaca caaaagaata aaacatcaca ctcagcgccg acttgcggtc
     8821 gtgcaggcgc ataaaccgcc aactcaaagc tgcatatcct gtgccc
//
LOCUS       DAORLF010000176         8813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4298_length_8813_cov_1.684289, whole genome shotgun sequence.
ACCESSION   DAORLF010000176 DAORLF010000000
VERSION     DAORLF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_8813_cov_1.684289"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1428
                     /locus_tag="PK198_14150"
     CDS             67..1428
                     /locus_tag="PK198_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF39927.1"
                     /translation="MENAQKIQHLYARAGFGLSPTEWNRRREQSLNEALQDLFSKPND
                     VRELPVPALVISETTANNNGVQQKERQLVAAIGIDWLNRMAAPLEDALLERMCLFWHG
                     HFACDIRIGLPAGQYLNTLRRHALGNFRDLVTAIAREPAMIRYLNNQQNRKERPNENF
                     ARELMELFTIGRGHYSEQDVKEAARAFTGWSSDRTGQFIFRRLLHDFDSKIFMGRTGY
                     FDGDDVIHILLEQKQTARFICQKIYRYFVNEQPDEQRVEAMTEVFFADYDIARLMRFV
                     LESDWFYEDRHIGSKIKSPVELTAGILRQLGVRHCNPAALIGIHRAMGQVLFHPPNVA
                     GWPGGKSWIDNSTLMMRLQLGAAFCMASDFDLALKTELDEQDRRFVRRLEAETDFAPL
                     ADLCRQVPEKEYFDVLSSFLLLPDVSRHRSTVEAYVPAGAPQERIRQLTARLMSLPEY
                     QMC"
     gene            1474..>2038
                     /locus_tag="PK198_14155"
     CDS             1474..>2038
                     /locus_tag="PK198_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HRF39928.1"
                     /translation="MHRRNFIKSSVLASTACMVPAFLSHTQPGRNLWGSRSGKVLVVI
                     QLSGGNDGLNTVIPYEDAAYYQLRPTLGIAKNEVLKISELIGLHPALAPLEKLYREGG
                     VSIVNNVGYPNPDRSHFRSMDIWHTASDSSAYWNTGWLGRYLDHECAGKPAYHALEVD
                     DGLSLALKGEHRSGFAMSSAQRIQRAAR"
     assembly_gap    2039..2048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2049..>2300
                     /locus_tag="PK198_14160"
     CDS             <2049..>2300
                     /locus_tag="PK198_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HRF39929.1"
                     /translation="LQAIDQAPSTEDHKVAYLYKTLRETRQSAEYLYQQSKVHKSSVA
                     YPDSKFATDLRQIAELMTADTDTRIYYVSLGGFDTHAGQL"
     assembly_gap    2301..2413
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2414..2664
                     /locus_tag="PK198_14165"
     CDS             <2414..2664
                     /locus_tag="PK198_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HRF39930.1"
                     /translation="RRARQNAGGGTDHGTANNVFLMGGQLRQTGLYNPGPDLSRLQDD
                     DLMFDIDFRRIYATVLDRWLEADAAQILQGRFELLGCV"
     gene            2743..3192
                     /locus_tag="PK198_14170"
     CDS             2743..3192
                     /locus_tag="PK198_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39931.1"
                     /translation="MDIKTIQNQLTEKHQLFSDFVGSLSSDEFSFRKSDKWTAGEQLE
                     HILLSVRPLRQALSLPKFMLKLIWGQSNRAGRDYEGLVNKYLSKLESGGRATGRFIPK
                     AVQETESAGRSTMSCAGYAPALTVLRKKNSISMFCLIHCWANSPCAR"
     gene            complement(3333..3713)
                     /locus_tag="PK198_14175"
     CDS             complement(3333..3713)
                     /locus_tag="PK198_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39932.1"
                     /translation="MKKQLILLLFICIAAAFATEAQAQQTRRADRREDVRDRRENVRD
                     RSENRRDRREDVRDRREDVRDAREDVRDAQHQGGVLDRMEDRRDAREDVRDVREDRRD
                     RREDVRDRSENRRDRRENRRDRRD"
     gene            complement(3984..4463)
                     /locus_tag="PK198_14180"
     CDS             complement(3984..4463)
                     /locus_tag="PK198_14180"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRF39933.1"
                     /translation="MKIDKVFENLESSDKVPRLTLKFEPSTISRVGLKDAEKIADFVR
                     QTFDPGEMGLKEEMILIPLDSLMRPICWYRLATGGRDTVSVDANLILMVCIAVGADGL
                     IIAHNHPDNVAIPSEEDIESAHTLRRKAHYLGLRYVDDIIITREDYYSLAENDLIWE"
     gene            complement(4571..>5418)
                     /locus_tag="PK198_14185"
     CDS             complement(4571..>5418)
                     /locus_tag="PK198_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39934.1"
                     /translation="AANTKLKGIEARERSTGQSFSTQKASIKQSVADAAAAHAAKLAA
                     LTETGAKEMRALQDEHNALETDLMRERAEWLAALKQGNKEAEAKRQKQVSTWGSSVAW
                     FTVLCLAVLLLAVALQEVHAHGSEIQEEALPNEYYFRQSPLAAFMAAVSERFHAKVFA
                     IIKRIEDGTPEPPEPGAPPTVYEYTQTMQRRPIGFKPIPIHRKPEDTHVGDGSSAKEA
                     LKNLTRPVAYETRSAAHKFKDCLHCGAQFIPNHKVQKYCGEPCRIQAWEAKNGRKPYM
                     KKGTK"
     assembly_gap    5419..5481
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5482..5978)
                     /locus_tag="PK198_14190"
     CDS             complement(<5482..5978)
                     /locus_tag="PK198_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39935.1"
                     /translation="MKFRFTLPTNEEFFQRYAGLIPTLSKLGYLAQVVSALTEVGILF
                     ALILNAVADIMPPGLATMTGITGAILATAFIEIGLRKFLPFSARQILNKRFAGLDLVM
                     SVAIFTASLLLLTASGALSFYGSKETVAAVVPPAQTEGTGAIDTTVNSHRQSNLKSYQ
                     RDSAA"
     gene            complement(6059..6292)
                     /locus_tag="PK198_14195"
     CDS             complement(6059..6292)
                     /locus_tag="PK198_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39936.1"
                     /translation="MQPTIFTKRRDAIAAMVLQGLTSDENNLSYDPKLPGTGYDQLAE
                     NAMRLAEALERVIEARDAPAREPQSIDETDVPK"
     assembly_gap    6407..6442
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6443..6738)
                     /locus_tag="PK198_14200"
     CDS             complement(<6443..6738)
                     /locus_tag="PK198_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39937.1"
                     /translation="MIYNTLSVPGMFPVCSRSKRKLLIFSIVKFSLCSRCVPGFDLFL
                     KSEKITTTRNCEKYRPNNFLQQRGRFEVADLQNVPNSRQRPGTKCPIPGTHREQ"
     gene            complement(6755..>8079)
                     /locus_tag="PK198_14205"
     CDS             complement(6755..>8079)
                     /locus_tag="PK198_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39938.1"
                     /translation="DGSTYLQADEYGIVRAGEKLYYLPAFSKVNELAEREYHNERKVL
                     FRPGKATFEQWAQQLITVFGDNAVIGICFVVAALFRDIIFAHVNAFPILFLFGPRGTG
                     KTTYRLGLKVLFGNYGPNDAIGLGSQSSPKGFARKLAQIRNGLEAFEEYKNRINPALI
                     EMLKNIYDGIGYERAQSTNDNRTHATPVNSAVIVAGQEMPTKENALFSRVLMLSFGKT
                     SHTDEAKREFHALETLIEDGLGNVLIEIITHREAVSAQFEKSFSEVYAHLRKDHTTNS
                     MDERSLSNVAALLAPMKLLAERLAFPFSYAEAYKAFRSRLLSQNEQMAQSSEVVQFWR
                     IVGVLIDNGQIRANHDYKISEGRIAIFMEGIFRQYYKHCTETGITPLDQATLESYLSM
                     QPYFRRPENDPGRIKTKVRMENGLQRRCLVFEENFTESEFLKNTGNID"
     assembly_gap    8080..8089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8090..>8813)
                     /locus_tag="PK198_14210"
     CDS             complement(<8090..>8813)
                     /locus_tag="PK198_14210"
                     /inference="COORDINATES: protein motif:HMM:NF020291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HRF39939.1"
                     /translation="HHPVNGGRNAFVFAFACELCRFGVPKSTAIAECQAYIDTDFSET
                     EIGKTIESAYSSKRFGEFTDRQLIAYRNRLSGRATQHTIAAAIVPAAAPMDATPAPVA
                     AAIEKRKAPEPEPEPPLYLGENCYLFEENKGKTKSKERISNFAIVPLYLLLDSHDPKR
                     VWLLRNMQEEETELCFPSRYLSSPKDFCALIEGKGNFVFSANMKQLATLKEYLYPKEQ
                     KAQEIGTLGHQPETGYYAFAQGI"
BASE COUNT         1950 a         2208 c         2233 g         2190 t
ORIGIN      
        1 tctaatcctt tggcatagag aaaatgaaca tttatttttg ccccactact tatgatctgc
       61 cgaaacatgg aaaacgctca aaaaattcaa catctctatg cgcgcgccgg cttcgggctg
      121 agtccgaccg aatggaatcg gcgacgcgag caatcgctca acgaagcgct tcaagacttg
      181 ttttccaaac caaatgacgt cagggaactg cccgtacccg cgctcgtcat ttcggaaaca
      241 accgccaaca acaatggggt gcagcaaaag gaacgacaac tcgtggcggc aatcgggatt
      301 gactggctta atcgcatggc cgcccctttg gaggatgcgc ttttagagcg gatgtgcctg
      361 ttttggcacg ggcattttgc ctgcgacatc cgcatcggtt tgccggcagg ccaatatctg
      421 aacaccttgc gccggcatgc gctgggcaat tttcgcgacc tcgtgacggc cattgcccgc
      481 gaaccggcca tgattcgcta cctcaacaac cagcaaaacc gcaaagaacg ccccaacgaa
      541 aacttcgccc gcgaactcat ggagcttttt accatcgggc ggggccatta ttcggagcag
      601 gatgtgaaag aggcggcacg ggccttcacc ggctggtcga gcgaccggac ggggcagttc
      661 attttcaggc gtttgttgca cgatttcgac tccaaaattt tcatggggcg cacaggttat
      721 ttcgatggcg acgatgtcat ccacatcttg ttggagcaaa agcaaacggc tcgtttcatt
      781 tgtcaaaaaa tataccgcta tttcgtcaac gagcaacccg acgagcagcg ggtggaagcc
      841 atgacggagg tcttttttgc cgattatgac atcgcccggc tcatgcgctt cgtgttggaa
      901 agcgactggt tttacgaaga ccggcacatc ggcagcaaaa tcaaatctcc tgtagaactg
      961 acggcgggca tcctgcggca gttgggggtg cgccactgca accccgctgc gctcatcggt
     1021 atccaccggg ccatgggaca ggtgttgttt cacccgccca acgtggcagg ctggcccggc
     1081 ggcaagagct ggattgacaa ttctaccctg atgatgcgct tgcaattggg cgctgctttt
     1141 tgtatggctt ccgactttga tctcgccctc aaaacagaat tggacgagca ggatcgccgt
     1201 tttgtgcgaa ggctggaagc ggaaaccgac ttcgctccct tagcagacct ttgcagacaa
     1261 gtgcctgaaa aggagtactt tgatgtgctg tcgtccttcc tgttgttgcc cgatgtgtcg
     1321 cgacaccgga gtacggtaga agcctatgtt ccggccggcg cgcctcagga acgcatccga
     1381 caattgaccg ccaggctcat gtcgctgccg gagtatcaaa tgtgctgaag cagctcgcag
     1441 ctttttcaag ttacattttc aaccaactac cacatgcaca gaagaaattt catcaaatcg
     1501 tctgttttag cctccaccgc ttgcatggtg ccggcgtttt tgagccatac ccaacccgga
     1561 agaaacttgt ggggcagccg cagcggcaaa gtactggtag tcattcagtt gtcggggggc
     1621 aacgacggcc tcaataccgt aattccttac gaagatgcgg cttactatca actgcgcccc
     1681 acgctgggta ttgccaaaaa tgaggtattg aaaatcagcg agttgatcgg cttacacccg
     1741 gcgctggcgc ctttagaaaa actctaccgc gaaggaggcg tgagcattgt caacaacgtg
     1801 gggtatccca atccagacag atcgcacttc cgctcaatgg acatctggca caccgcctcc
     1861 gacagcagcg catattggaa cacgggttgg ctggggcgct acctggacca cgagtgcgcg
     1921 ggcaaacccg cctatcacgc gctcgaagtg gatgacggtc tcagcctggc gctcaaaggg
     1981 gaacaccgca gcggatttgc catgagcagc gcccaacgca tccaacgcgc cgccaggcnn
     2041 nnnnnnnntt gcaggccata gaccaggctc cttcaacaga agatcacaag gtggcgtacc
     2101 tgtacaaaac gctccgggaa acgcggcaat cagcggaata cctctaccag cagtccaaag
     2161 tacacaagtc ctccgtggcc tatccggaca gcaaattcgc caccgacctc cgccaaatcg
     2221 ccgaattgat gaccgccgat accgacaccc ggatttatta tgtgagtctg ggcggattcg
     2281 acacccatgc cgggcagctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnggcggcg ggcaaggcaa aatgccggcg gcggcaccga ccacggcacg
     2461 gccaacaatg tgttcctcat gggcggccaa ctcagacaaa ccggcctgta caatcccggc
     2521 cccgacctga gtcgcttgca agacgacgac ctgatgtttg atattgattt ccggcgtatt
     2581 tacgccaccg tactcgatcg ctggctggag gccgatgcag cacagatttt gcagggcagg
     2641 tttgagttgc tggggtgtgt ttgagggagg atttattgcc gggacttggt ttttgggagg
     2701 gaagaggtat atttgtgata tctccacaaa taaaatggct tcatggacat caaaaccatc
     2761 caaaaccaac tcaccgaaaa gcaccaatta ttttcggatt tcgtgggcag tctgagcagc
     2821 gacgaattct ccttcaggaa gtccgacaaa tggaccgccg gagagcaatt ggagcatatt
     2881 ctactcagtg tcaggccgct gagacaggcg ctgtctttgc cgaaattcat gttgaagctc
     2941 atttgggggc agtccaacag agccggcaga gactatgagg gtttggtgaa taagtatctc
     3001 tccaaattgg aaagcggcgg aagagctacg gggcgcttta ttcccaaggc cgtgcaagaa
     3061 accgaatccg cagggcgctc gaccatgagc tgcgccggtt atgcgccagc cttgacagtt
     3121 ttacggaaaa agaactcgat cagtatgttt tgcctcatcc attgttgggc aaactcaccc
     3181 tgcgcgagat gatgttcttt acgatttatc atgtgcagca ccacgaagtg ctgactaaga
     3241 aaaacctgga acagtagtcc gcggcaccga caaaaaaagg cagaagacca tcacagtcct
     3301 ctgccttttt tgtttttggg caaaaccccg gctcaatccc ttcggtcccg gcggttttcg
     3361 cggcgatcac gccggttctc cgagcggtcg cgtacatctt cgcggcggtc gcggcggtct
     3421 tcgcgtacat cgcgcacgtc ttcgcgggcg tcgcggcggt cttccatccg gtcaaggacg
     3481 ccgccctgat gttgagcgtc gcgcacatcc tcgcgggcat cgcgcacgtc ctcgcggcgg
     3541 tcgcgtacat cttcccggcg gtcgcggcgg ttttcagagc ggtcgcgtac attttcgcgg
     3601 cggtcgcgta cgtcctcgcg gcggtcggcg cggcgtgttt gctgggcttg ggcttcggtg
     3661 gcaaaagctg cggcgatgca gatgaagagc aacagaatga gctgtttttt catggtcttt
     3721 tcaagttttg gtgatgtgag atgttgttga gacttgcgag aggaggccgg gtttaagctg
     3781 gatgaataaa agttatcacc attgtgtcac ccgcctgaaa attcagggca taaaaaaagc
     3841 gcaattattt gtatttcaaa caattgcgct tttaaagaag tgatcccgac agatattggt
     3901 taaatcgctg taaatcaatg aactaacagg ttcaaaaatg ctgggtacaa ataccggtac
     3961 aaaacggctt tcaaaatacg cgtttactcc caaatcaaat cgttttcggc caggctgtaa
     4021 taatcctccc tggtgataat gatgtcgtcc acataccgca gtcccaagta atgagctttg
     4081 cgcctgagtg tgtgagcgct ctcaatatct tcctcggaag gaatggccac attgtccggg
     4141 tgattgtggg caataatgag accatcggcg cccactgcta tgcacaccat caaaatcaaa
     4201 ttggcgtcaa cggaaacagt atcgcgccct ccagttgcca aacgatacca acaaatgggc
     4261 ctcatcaggg aatccaaagg aatcaatatc atttcctcct ttaaccccat ttctccaggg
     4321 tcaaatgtct gccggacaaa atcggcgatc ttttcagcgt ctttgaggcc aacccggctt
     4381 attgtcgaag gctcaaattt cagggttaac ctgggtactt tatcgctgct ctccaggttc
     4441 tcaaatactt tgtcaatttt cataatcgta gttgttttgg ttcaaaaatg aatgtgcaat
     4501 gaatgtgcag tgaatgtgta atgagtatgt aatgaatgtg tattgaatgt atgtcactca
     4561 ttatatacgt ttactttgtc cctttcttca tgtatggttt gcgcccgttt ttggcttccc
     4621 atgcctgaat acggcatggc tccccacaat acttttgaac tttatggttg gggataaatt
     4681 gggcgccaca atgcagacaa tctttgaact tatgcgcagc gctacgagtc tcgtatgcaa
     4741 cgggtctcgt tagatttttc agggcctctt tggcgctact gccgtcgccg acgtgcgtgt
     4801 cctccggttt tctgtgtatt ggtatcggtt taaatccgat ggggcggcgc tgcatcgtct
     4861 gggtatactc gtatactgtt ggcggcgctc cgggttctgg tggctccggt gtgccgtcct
     4921 cgatgcgttt gatgattgca aatactttgg catggaagcg ctccgatacg gccgccataa
     4981 atgccgccaa cggcgactgc cggaaatagt actcattggg cagtgcctcc tcttgtatct
     5041 cgctcccgtg tgcatggact tcctgcaggg cgaccgccag caacaaaaca gctaggcaca
     5101 aaacggtaaa ccaggccaca ctacttcccc atgttgatac ctgcttttgc cgtttggctt
     5161 cggcttcttt attgccctgt ttcaatgctg ccaaccattc ggcgcgttct cgcatcaaat
     5221 cagtctccag ggcattatgt tcgtcctgga gcgctcgcat ttccttcgcc ccggtctcag
     5281 taagggcggc cagcttcgca gcatgggcgg cggcggcatc ggcaacggat tgtttgatgc
     5341 tcgctttctg ggtgctgaaa ctttggccgg tactacgctc ccttgcctca attcctttca
     5401 gcttggtatt ggccgcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn natggcggca ctgtcccgtt ggtaggactt taggttactt
     5521 tggcggtggc tgttgactgt ggtgtcaatg gcgcccgttc cttctgtttg tgccggtgga
     5581 acaacggcgg caacggtctc tttgctgcca taaaacgaca gggcgcccga tgcagtgagt
     5641 aacaacagtg aagcggtaaa aatggctacc gacataacca agtcaagtcc tgcaaagcgt
     5701 ttgttcaaaa tctgccgggc gctgaacggt aggaacttgc gcaggccgat ttcgataaag
     5761 gcagtagcca atattgcacc tgtgataccg gtcattgtag ccaggccggg cggcatgatg
     5821 tcggcaacgg cattgaggat gagggcaaac aaaatgccca cttcggtaag agcgcttacc
     5881 acctgggcga gataacccaa ttttgagagg gtagggatga ggccagcgta ccgctgaaaa
     5941 aattcctcat tggttggaag tgtgaaacgg aatttcataa atttgcctga ttttcaattg
     6001 tttgaaagtt ggccgggcgc tgttcttgta ggtgggcgcc cggccttttt gttttcgatc
     6061 atttcggtac gtcggtttcg tcaatacttt gcggctctcg cgccggtgcg tctcgtgctt
     6121 caattactct ctccagggcc tcagccaatc tcatggcgtt ttctgccagt tggtcatatc
     6181 cggtacctgg tagctttggg tcgtagctga gattgttttc atcggatgtc aaaccctgta
     6241 aaaccatggc tgcgatggcg tctcgtcttt tggtaaaaat cgttggttgc atttcatcag
     6301 tttttcacat ttattcccaa aatcgggcaa aaaatttttc taaaaaaaaa acttgtgttt
     6361 ttaccgggaa caccgggaca ctgggccttt ttttgacata aagaccnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnttttgttc ccggtgtgtt cccggtattg ggcattttgt
     6481 tcccggtcgt tggcgtgaat ttggcacgtt ttgaagatcg gcaacttcaa atcggcctct
     6541 ttgctgcaaa aaattatttg gcctgtattt ttcacaattt cgagtagtgg taattttttc
     6601 agatttcaaa aacaggtcaa aaccgggaac acaccgggaa cagaggctaa attttactat
     6661 actgaaaatc aacaatttac gttttgaccg ggaacatacc gggaacatac cgggaacact
     6721 taaagtattg tatatcatat tattataatt ttttttagtc aatgttcccg gtatttttca
     6781 aaaattcgct ctccgtgaaa ttttcttcaa acacaaggca gcgtctttgt aggccatttt
     6841 ccattctcac tttggttttt atgcgccctg gatcgttttc cgggcgcctg aaatacggct
     6901 gcatcgaaag gtagctttca agggtagctt gatctaaagg agtaataccg gtctctgtac
     6961 aatgtttgta gtactgcctg aaaatgcctt ccatgaaaat ggcaattctt ccctccgaaa
     7021 ttttgtagtc atggttggcc ctgatctggc cgttgtcaat cagtactccg acaattctcc
     7081 aaaactgtac tacctcgctg ctttgtgcca tttgctcatt ttgtgagagc aggcgtgagc
     7141 ggaatgcttt gtaggcttcc gcatagctga aggggaaagc cagcctttca gccaatagct
     7201 tcatgggcgc caatagtgcg gcaacgttgc tcaatgagcg ttcgtccatg ctgttggtgg
     7261 tgtggtcttt tctcagatgc gcgtacacct ctgaaaagct cttttcaaac tgagcgctga
     7321 cggcttcccg gtgggtaatg atctcaatga ggacatttcc caaaccatcc tcgatgagcg
     7381 tttccagggc gtgaaattct cgcttcgcct cgtcggtgtg cgaagttttg ccaaacgaca
     7441 acatcagcac ccggctaaac agtgcattct ctttggtagg catttcctgg ccggccacaa
     7501 tgacggcaga gttcaccggt gtagcgtgtg tgcggttgtc gttggtgctt tgggcgcgtt
     7561 cgtagccgat gccgtcatag atgtttttca gcatctcgat caatgccgga ttgatgcggt
     7621 ttttgtactc ctcgaaggct tccaggccgt tcctgatctg tgccagcttc cgggcaaaac
     7681 ctttcgggct gctttggctc cccaggccga tagcgtcatt cggcccatag ttgccaaata
     7741 ggactttgag gcccaaacga taggttgttt tgcccgttcc gcgtggccca aagagaaaga
     7801 gtatcggaaa ggcgttgacg tgggcaaaaa tgatgtcccg aaacaatgcc gccactacaa
     7861 agcaaatccc gataacggcg ttgtctccaa agacggtaat aagttgttgg gcccactgct
     7921 caaaagtagc cttaccaggg cgaaacaaaa ctttccgctc gttgtggtat tcccgttcgg
     7981 ccaattcgtt gaccttgcta aatgccggca gatagtagag tttttcccca gctcgaacga
     8041 tgccgtattc atctgcctgc aggtaggtac tgccgtcatn nnnnnnnnna aatgccctga
     8101 gcaaaggcat agtagccagt ttccggctga tgccccaacg tgcctatttc ctgcgctttt
     8161 tgctcctttg ggtagaggta ctctttgagt gtagccaatt gcttcatatt ggccgaaaac
     8221 acaaagtttc ctttcccctc gatgagggcg caaaagtcct ttggcgaact caggtaccgc
     8281 gatggaaaac aaagctccgt ttcttcctcc tgcatattcc gaagcaacca aacccgttta
     8341 gggtcgtgac tgtccaagag caaataaagc ggcacaattg caaaattgct gatgcgctct
     8401 ttactcttgg ttttgccttt gttttcctca aacaggtagc aattttcgcc caggtacaaa
     8461 ggcggttccg gttccggttc cggcgctttc cgcttctcaa tggcggcggc aacgggcgcc
     8521 ggtgtggcgt ccatcggcgc ggcggcggga acaatggcgg cggctattgt atgttgtgtt
     8581 gcgcggccgc tcaggcggtt cctgtacgca attagttggc gatctgtgaa ctctccaaaa
     8641 cgcttagatg agtaggcgct ttctatggtt ttgccaattt cggtttctga aaagtccgta
     8701 tcaatgtacg cctgacattc agcaatggcg gtacttttcg gcactccaaa acggcacaat
     8761 tcgcaggcaa aagcaaatac aaaggcattg cggccgccat tgaccgggtg atg
//
LOCUS       DAORLF010000177         8773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4330_length_8773_cov_1.839183, whole genome shotgun sequence.
ACCESSION   DAORLF010000177 DAORLF010000000
VERSION     DAORLF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4330_length_8773_cov_1.839183"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK198_14215"
     CDS             <1..192
                     /locus_tag="PK198_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39940.1"
                     /translation="IRRQDVGFPSNKMVFLRAGGMARYHFPPSNKIGLFVSAAQVLEG
                     RNVGQSFLWSGGVLFQFYK"
     gene            226..1224
                     /locus_tag="PK198_14220"
     CDS             226..1224
                     /locus_tag="PK198_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39941.1"
                     /translation="MKSFSILICLLACIAGLWVSCEKNIPTQLEFSHYEFSESDANGG
                     TWKPVLLTSASQIGIPAPQSTSSASYLAELADVKAASSQLTAQQRAAVEYWGNNGLLR
                     WNEIARELAAKYNLPPAPNPDGTYGAPDPANPDRYPQFPFAHPPYACRMFAYWSAAQM
                     DALISAWHYKHLYNRPAPYKVDNTIQPHLPVSDLPSYPSDAAVVAAVSEAILGAMFPL
                     EKAYLNQRALEHKNAALWSGSNVWSDVTAGDSLGRAVAAVFLRLHRRHEKCAGTQACF
                     RFISRRCIVVQRHEMGEPRRASPARRHHAIVWSGQTLVRTQCRGSTPATAAGNRLS"
     gene            1142..1762
                     /locus_tag="PK198_14225"
     CDS             1142..1762
                     /locus_tag="PK198_14225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF39942.1"
                     /translation="MFGRVKLWCVPNVEAVRPPQPPAIGSPEFEQAAAELRDIAKNLT
                     TEQRRIANFWSDGIGTYTPPGHWNRRATELIVKNRYNPLRSARTYAYMNMAVMDAGIS
                     CWDAKYYYHYPRPIQAIPGFKTILGTPNFPAYTSGHSTFSAAAATVLGHIFPSEKQTL
                     DAWAKEAADSRLYGGIHYRFDSEEGLKQGRAVAAYSVAIAIADGAE"
     assembly_gap    1820..1834
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1839..>2179)
                     /locus_tag="PK198_14230"
     CDS             complement(1839..>2179)
                     /locus_tag="PK198_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRF39943.1"
                     /translation="DWKIGIALITSFAAREVFVGTMATIYSIGSSDDELSVRQRMAKE
                     INPRTGLPVYGIATAFSLLIFYVLAMQCMSTLAVVKRETNSWKWPLVQFFAMTTFAYL
                     ASWATYQLLS"
     assembly_gap    2180..2220
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2221..3976)
                     /gene="feoB"
                     /locus_tag="PK198_14235"
     CDS             complement(<2221..3976)
                     /gene="feoB"
                     /locus_tag="PK198_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRF39944.1"
                     /translation="MSGRSSFTIALVGNPNCGKSSIFNRLTGLNQKIGNFPGVTVDKK
                     QGVLRLSPETEANLIDFPGAYSFYPNSQDERIVAQTFAQTDSPDFPDAVVYVADVTRL
                     EKHLLLFSQIRDLGLPVVLALNMADVAQREGLQVDTGALAGFFKTPVVLLSGRTGAGI
                     PELRSALLQICRQGQPAGAPHHSYQLSPQEHTASRTVTALMPGVSGYQALLIAHHYEW
                     LPFLSEQQREQIAAGLGDIQFNSVKSQVEETLERYSIFSQVVSSALRRPDVPNGRLTA
                     AMDRVLTHRVAGPIIFFALMALIFQSIFAWATYPMDWIEQGFGMAEGWLAEVLPEGWV
                     NSLVTEGILAGLGGILVFVPQIAILFFLISLLEEVGYMARAAFIFDRLMRWFGLNGRS
                     IVALISGGACAIPAIMSTRTISNWKERLITILVTPFISCSARIPVYTVLIGFAVPSKT
                     MGGFFNLQGLAFLGLYLLGLVAALLAGWTLKTLLKSKERSFLMLELPQYRTPVLRNVG
                     LTVWEKVRAFVVEAGTIIMLISLILWGLSSYGPSNKMQLAEQEAIELAASQQLDETGT
                     QDLIAAHRIEASWAGHLGK"
     gene            complement(3987..>4171)
                     /locus_tag="PK198_14240"
     CDS             complement(3987..>4171)
                     /locus_tag="PK198_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRF39945.1"
                     /translation="VVSHFGDQHLAGRLMAMGVLPGSRVEVLRSAPFGGGFYVKADNL
                     LLALRSEEAQSILIRQ"
     assembly_gap    4172..4208
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4632..>5027
                     /locus_tag="PK198_14245"
     CDS             4632..>5027
                     /locus_tag="PK198_14245"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF39946.1"
                     /translation="MKKLLFVFMLLLGSAAALTAQRAITGTVTDQNKEPLIGATILVE
                     GTSVGTVTDALGEFGLTVPAGSSTLLISYTGYTTRRVELTAANNYSLTLETDAVTLSD
                     VVVTAYGTTRKEALTGSVGSIKSEAIALRP"
     assembly_gap    5028..5056
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5057..>6054
                     /locus_tag="PK198_14250"
     CDS             <5057..>6054
                     /locus_tag="PK198_14250"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF39947.1"
                     /translation="GVITSTANGQPGSGLDIRIRGFGSINATQEPLYVVDGVPYVGGT
                     SNINPDDVESISILKDASATALYGSRAANGVVMITTKRGKKGRNNVSLKVTQGFATRG
                     LPEYELVDAFEYYPITWEGYRNQLVYPASGTGISRDSAARVASGLTNRTSVAGLLAYN
                     PFNVPANQIVGVDGKINSNAQLIYGDDLDWTKDLMRTGDRKDYVVSFNGGADKMDYFL
                     SAGYLKEDGYTINSDFERFTARLNVNVQPKEWLRTGLNISANHTTSNTANDDGSTSFV
                     NPFFFSRNIGPIYPVYAHNMTTGEYLIDPATGQRFWDLGNFGNSGLGIPNRPGNGF"
     assembly_gap    6055..6092
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6093..7817
                     /locus_tag="PK198_14255"
     CDS             <6093..7817
                     /locus_tag="PK198_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF39948.1"
                     /translation="AETTLNSLLFKRTVLSGRSTTDIIFLKNFKFTNSLGVDFQYQAN
                     SNFENTLVGDGAPAGRSRRQFGSNIGLIATQLLSYVNDFGKSNIDILVGHESYNQRLT
                     DLNGFKQGQSLSGNTELNNFTTINSLTSALDFYRIESYFSRASYNYDDRYVLTASLRR
                     DGNSRFADGSRWGTFWSVGGGWNLHKESFFNASWVNRLKLRSSYGVTGVADGIGYYAY
                     QGLYGFANNANEPGILQSTTAFLNTDLSWESNTQFDLGLDFGLFKDRISGSVEFFNRE
                     SSDLLFAVPQPLSSGINSTLQNTATLFNRGIEANLSIDVVRTKDFLWNMNFNASTLTN
                     EITKMPESVPEFITGTKKYAVGQSIFDYWLRTYYGVDPTDGAALYVANDPTITANRRI
                     IGTDTLTTSINNGKFEYNGSVIPDLYGSFSPTIRYKGLSLSALFTFQLGGLTYDALYQ
                     SLMSSSTVGGALHKDILNRWQNEGDITDVPRMDQGRGPDFNATSSRWLVDASFLNIRN
                     VNLSYNLPQSLLGKMKLTNANVFVAMENVAFFSRRVGMNNQQSFSGVTSNAYPPARVF
                     SGGFNINF"
     gene            7831..>8773
                     /locus_tag="PK198_14260"
     CDS             7831..>8773
                     /locus_tag="PK198_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF39949.1"
                     /translation="MKKSMYKITAIFAILFLASACEKEFLETYPTTAVSTNDVLLSTQ
                     NAIAALNGIHRNLYTQSGTVQGHAGEGCNNMFRDLIGEDIIYPLANGSTGFVGLMRWQ
                     THRNVNDATLAYMYRFYYRIISNANVLINGIEKVPGPQADKDMIKGQALAYRGWSHFQ
                     LVQLWGERYDGSRTNSQLGVPILLENTLVGQPRATVEEVYTQINKDLDEAITLLAGYN
                     RTGSAAKSNINVNVAQGFKARVALTQQKWADAAAAAAAARAGVSLMSNAQYTTGFNDI
                     TNPEWMWGSRQISDHNTFFWSYFAYMSANFNSTVIRTQ"
BASE COUNT         2121 a         2402 c         2193 g         1897 t
ORIGIN      
        1 atccgcaggc aagacgtggg ttttccttcc aacaaaatgg tgttcttacg agcaggcggt
       61 atggctcggt accactttcc gccctccaac aagatcgggc tgttcgtttc tgccgcacag
      121 gtgctggaag ggcgaaacgt ggggcaatct ttcctctggt cggggggagt tctgtttcaa
      181 ttttacaaat aatcctgatt tacctcaccc atcaatcaac aaaccatgaa atcttttagc
      241 atccttatat gtctcttggc ctgtattgcg ggcttatggg tttcttgcga aaagaatatc
      301 ccgactcaac tcgaattttc gcattacgaa ttttccgaat cagatgccaa tggcggtacc
      361 tggaaacccg tactgctgac cagcgcctcg caaataggca ttccggcgcc acaatccacc
      421 agttctgcat cctatctggc tgaattggcc gacgtcaaag ctgcctcctc ccaactgaca
      481 gcacaacaac gagccgctgt tgagtactgg ggcaacaacg gcctgctccg ctggaacgaa
      541 atagcccgtg aattggccgc caaatacaac ctgccgccgg cccccaatcc tgacggaacc
      601 tacggcgccc ccgatcccgc caacccggac agatacccac agtttccttt tgcacacccg
      661 ccttatgcct gtcggatgtt tgcatactgg agtgccgctc aaatggacgc gctgatttcg
      721 gcctggcact acaaacatct gtataacaga ccggcgccgt acaaggtgga caatacgatt
      781 cagccccatc tgcccgtgag tgatttgccg tcgtatccat cagatgccgc tgtggtggcg
      841 gctgtttcgg aagcgatttt gggcgctatg tttcccttgg aaaaagccta cctgaatcaa
      901 cgggcgctgg agcacaaaaa cgcggcttta tggtcaggct cgaatgtatg gagcgacgtg
      961 acggccggcg attctctggg cagagccgtg gccgctgtgt ttttgcgcct ccacagacgg
     1021 catgagaaat gcgcaggcac ccaagcctgt ttccgattca ttagccgccg ctgcattgtt
     1081 gtacagaggc acgaaatggg tgaacctcga agagcctccc cggcccgtag gcatcacgcc
     1141 attgtttggt cgggtcaaac tctggtgcgt acccaatgta gaggcagtac gcccgccaca
     1201 gccgccggca atcggctctc ctgaatttga acaagccgcc gcagaacttc gggatattgc
     1261 caaaaatctg accactgagc agcgccgcat cgccaatttc tggtccgacg gcatcggcac
     1321 ctatacgccg ccggggcact ggaaccgccg cgctacggag ttgattgtca aaaacagata
     1381 caatcccctg cgctcagccc gcacttatgc ctatatgaat atggcggtga tggatgcggg
     1441 catcagttgc tgggatgcca aatattacta ccactatccg cgccctatac aggcaatacc
     1501 gggcttcaaa accatcctcg gaacaccgaa tttccccgct tacacttcag gacactccac
     1561 gttctcagct gcagcggcta ccgtgctggg acatatattc ccatccgaaa aacaaacgct
     1621 ggatgcctgg gccaaggaag ctgccgattc ccggctgtat ggcggcattc attatcgttt
     1681 cgattctgaa gaaggactca aacaggggcg tgccgttgct gcgtactcgg ttgcaattgc
     1741 catagccgac ggggcggaat agatacaaaa gaaagttgac tctacggggc gcattttccg
     1801 gtgaatttac ctgtgccggn nnnnnnnnnn nnnntgggtc aactcaacaa ctggtacgtt
     1861 gcccaactgg ccaaataagc gaaggtcgtc atagcgaaga attgcacgag cggccatttc
     1921 caggagttcg tctccctttt cactactgcc agtgtactca tgcactgcat ggccagtaca
     1981 taaaaaatga gcagcgaaaa ggccgtagca atgccgtaaa caggcagccc ggtacgcgga
     2041 ttgatttctt tggccattct ttgccgcacc gatagctcgt cgtcgctgct gccgatgctg
     2101 taaatggtgg ccatagtgcc tacaaacacc tcgcgggcag caaaagaagt gatgagcgca
     2161 atgccgattt tccagtcaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 atttgcccag atgccccgcc caagaggctt cgatgcggtg cgcggcgatg aggtcctgtg
     2281 tgccagtttc atccaactgt tgtgaagcgg ccaactcaat ggcttcttgt tcggccaatt
     2341 gcattttatt agaaggcccg tacgaggaca agccccaaag aatcaatgaa atgagcatga
     2401 taatggtgcc ggcttccact acgaaagcgc gcactttttc ccacacggtg aggcccacat
     2461 tgcgcaatac cggcgtgcgg tactggggta gttccagcat gagaaagctg cgttctttgc
     2521 ttttcaacag cgttttgagc gtccagccgg ctaataatgc ggccaccaaa cccagcagat
     2581 aaagccccag aaacgccagc ccttgcaggt tgaaaaagcc gcccatcgtc ttggagggta
     2641 cggcaaaacc aatgagcaca gtataaacag gtatgcgcgc cgaacaactg atgaacggcg
     2701 tgaccaatat ggtgatgagg cgctctttcc aattgctgat ggtgcgcgta ctcatgatgg
     2761 cgggaatagc gcaggccccg cccgatataa gcgccacaat actgcggccg ttgagaccaa
     2821 accagcgcat cagtcggtca aaaataaaag cggcccgcgc catgtagccc acttcttcca
     2881 gcagagaaat gaggaaaaaa aggatggcaa tttgcggtac aaacaccaaa attccaccca
     2941 gcccggccaa aattccctcc gtaaccaagc tgttgaccca gccttccggc aatacctcgg
     3001 ccaaccaacc ctctgccatg ccgaaaccct gctcaatcca gtccatcgga taagttgccc
     3061 aggcaaaaat ggactgaaaa atgagggcca tgagggcaaa aaatataatg gggccggcaa
     3121 cgcgatgtgt cagtacgcgg tccatagcgg cggtgaggcg gccattgggt acatcagggc
     3181 ggcgcaaagc agacgaaaca acttgtgaaa agatgctata gcgctccaat gtttcttcca
     3241 cctgactttt gacgctgttg aactgtatat ccccgaggcc ggcggcaatt tgttcgcgtt
     3301 gctgttccga taaaaaaggc agccattcgt agtggtgagc gatcaataat gcctgatagc
     3361 cggatacgcc gggcatgagg gcggttaccg ttcgggatgc ggtgtgttct tgcgggctga
     3421 gttggtagga atgatgagga gcgcccgcag gctggccttg ccggcaaatt tgaagcaggg
     3481 cgctgcgcaa ttcggggatg cccgcgccgg ttcgaccgct gagtaagacg accggagttt
     3541 tgaaaaaacc agccaaagcc cccgtgtcca cctgtaagcc ttcgcgctgg gccacatcgg
     3601 ccatattgag tgccagaacg acgggaagcc caaggtctcg tatttgtgaa aacaggagca
     3661 aatgcttttc taaacgggtc acatcggcca catatactac agcatccggg aagtcggggc
     3721 tgtcggtttg ggcaaaggtt tgagccacaa tgcgctcatc ctgtgaattg gggtaaaaac
     3781 tatatgctcc gggaaaatca atcaagttgg cctctgtttc cggcgaaagc cgaaggacgc
     3841 cttgcttttt gtccactgtc acgccgggga aattgccgat tttctggttc aggccggtca
     3901 ggcggttgaa aatggaggat ttgccacaat tgggattccc gaccaaagca atggtaaaag
     3961 aagagcgacc ggacatgaca ggcaggctat tgtctgatga gaatagactg ggcttcctcg
     4021 ctgcgcaatg ccagcaacag attatcggct tttacataaa acccaccgcc aaagggcgcc
     4081 gaacgcagca cttccacccg gcttccgggc agcacgccca tagccatgag gcggccggcg
     4141 aggtgttggt ctccgaaatg ggataccacg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnncg agagggtaaa aaacgatgtt gcagcggcag ccatgtttcc tattttgatt
     4261 taatctaatt tacaattacc ccgcaaaaat agacgctttt ccgaattggc aaggtaataa
     4321 atatcacctg cacaaaaaat acctaaagcg aatggatgta cctattatta ggtattttat
     4381 aaagtaaagg caacccctcc tctgtggggc ttgtccgtaa aaagtcattc tatgtcaggg
     4441 cgccaacatt tgcaaaggca gcacaggcct gcactgaaac gatatgtatt acagcgcagg
     4501 catcattgca aaacaaaagt agaggaaggg gcggctaaaa gataattttt tcactcaagc
     4561 ggctcattcc tgaacgatag ttatactttt gctcactgga atttctattt acaaaccaaa
     4621 tacagtcaga catgaaaaaa ctgttattcg tgttcatgct cctgctgggc agcgctgcgg
     4681 cgttgacggc acaacgggcg atcaccggca cggtgaccga ccaaaacaag gaaccgctga
     4741 taggcgccac cattttagtg gagggcactt cggtaggaac cgtaacggat gcactgggcg
     4801 agttcggcct tactgtgccg gcgggttcca gcaccctgct catcagttac actggttaca
     4861 ctacccgccg ggtagaactc accgccgcca acaactacag cctgacgctg gagacagacg
     4921 cagttacact cagcgatgta gtggtaaccg cttatggcac cacccgcaag gaagccctca
     4981 ccggttcggt gggcagcatc aagtcggaag ccattgcgct gcggcctnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnncggg tgtcatcacg agtactgcca atggccagcc cggctcaggt
     5101 ttggatattc gtatccgcgg cttcggctcc atcaatgcta cccaagagcc gttgtatgtg
     5161 gtggacggcg tgccttatgt tggcggcacc tccaacatca acccggacga cgtggagagc
     5221 atcagtatcc tgaaagatgc ttctgcaaca gcattgtacg gctcgcgcgc agccaacggc
     5281 gtggtgatga ttacgaccaa acgcggcaaa aaaggccgca acaatgtgtc gctcaaagtg
     5341 acgcagggtt tcgctacccg cggcctgccc gagtacgagc tggtagatgc gtttgaatac
     5401 tacccgatta cctgggaggg ttaccgcaac cagttggtgt atcctgcttc cggcacgggc
     5461 atttccagag actcggctgc ccgcgtggct tccggcctca ccaaccgcac cagcgtggcg
     5521 ggtttgctgg cttacaatcc attcaatgta cccgccaacc agattgtagg ggtggacgga
     5581 aaaatcaatt ccaatgcaca attaatatat ggagatgatt tggactggac caaagacctc
     5641 atgcgcacgg gcgaccgtaa agactatgtg gtttccttca acggcggcgc cgacaagatg
     5701 gactatttcc tctctgccgg ctacctcaaa gaagacggct acaccatcaa ctccgacttc
     5761 gagcgcttca ccgcccgcct caacgtgaac gtgcaaccca aagagtggct ccgcaccggc
     5821 ctgaacattt cggccaacca caccacctcc aacacggcca acgacgacgg cagcaccagc
     5881 ttcgtaaatc cattcttctt ctcgcgcaac atcggcccga tttaccccgt gtatgcgcac
     5941 aacatgacca ccggggaata tctcattgac ccggcgacag ggcagcgctt ctgggatttg
     6001 ggcaacttcg gcaactccgg cctgggcatt cccaaccgtc cgggcaatgg ttttnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagagac tacgttgaac agcctgctgt
     6121 tcaagcgcac cgtattgagc ggtcgttcta cgacggacat tatttttctc aaaaatttca
     6181 agtttacgaa cagcttgggt gttgattttc aataccaggc caatagcaac ttcgaaaata
     6241 ctttggtggg cgacggcgct ccggcgggta gaagccgccg tcagttcggc agcaacatag
     6301 gtctcattgc tacccaattg ttgagctacg ttaacgattt cggcaaaagc aatatagata
     6361 ttttggtggg acacgaatcc tacaaccagc gactcaccga cctcaacgga tttaagcagg
     6421 gccagtccct ttcgggcaat accgagttga acaactttac gaccatcaac tcattgactt
     6481 ctgccctcga cttctaccgc attgagagct atttctcaag agcttcttac aactacgacg
     6541 accgctatgt cctcacagcc agcttgcgca gagacggcaa ttcccgtttc gcagatggct
     6601 cccgctgggg tactttctgg tcggtaggcg gcggatggaa cctccacaag gagtccttct
     6661 tcaatgcgtc atgggtaaat cgcctcaaac tgagaagctc ttatggcgtt accggcgttg
     6721 ccgacggcat cggatattat gcttaccaag ggctttatgg ttttgccaac aatgccaacg
     6781 agccggggat tttgcaaagt acgactgcat ttctcaatac cgatttgtca tgggaaagca
     6841 acacacagtt tgacttagga cttgactttg gtctgtttaa ggatcgcatc agcggctctg
     6901 ttgaattttt caaccgcgaa tccagcgatc tgctcttcgc cgttccgcag cctttgtcca
     6961 gcggcatcaa cagcacactc caaaacacag ctaccttgtt caaccggggt attgaggcca
     7021 acctgagcat tgacgttgtg cgcaccaagg acttcttgtg gaatatgaac ttcaacgctt
     7081 ctacgctgac caacgagatc accaagatgc cggaatccgt tcccgaattc atcaccggca
     7141 ccaagaagta tgccgtgggc cagtctatct tcgactactg gctgcgcact tattatggcg
     7201 tggatcctac cgacggcgct gcattgtatg tggccaacga tcctaccatt acggccaacc
     7261 gccgcatcat cggcacggat acgctcacta cgtccatcaa caacggcaag tttgaataca
     7321 acggcagtgt aattccagac ctgtatggca gtttctcgcc gacaatccgc tacaaaggct
     7381 tgtccttgag cgccttgttt acattccaac ttggaggttt gacgtatgac gcgctttacc
     7441 agtcgctcat gtcttccagc acggtaggcg gcgctttgca caaagacatc ctgaaccgct
     7501 ggcaaaatga aggcgacatc actgatgtgc cgcgtatgga ccagggcaga ggccccgatt
     7561 tcaacgccac gtcttcacgc tggttggtgg acgcttcttt cctgaacatt cgcaacgtga
     7621 atctgtcata caatttgccg caatcgctgc tgggcaagat gaagctgacc aacgccaatg
     7681 tatttgtagc tatggaaaac gtagctttct tctccagacg cgtaggcatg aacaaccagc
     7741 aatctttttc aggcgtgacg tcgaatgctt atccgccggc cagagtgttc tcgggcggtt
     7801 tcaacatcaa cttctaaaac aggtacaacc atgaaaaaat caatgtataa aatcacggcg
     7861 attttcgcca ttctttttct ggcctctgcc tgcgaaaaag aatttttgga aacatatcct
     7921 accacggcgg tatccaccaa tgacgtacta ctgagtacgc aaaatgccat tgcggcgctc
     7981 aacggtattc accgcaatct ttacacacag tcgggtactg tgcagggcca cgccggagaa
     8041 ggctgcaaca acatgttccg cgacctgatc ggggaagaca tcatctatcc gctggccaac
     8101 ggcagtaccg gtttcgtggg gttgatgcgc tggcaaaccc accgcaacgt gaatgatgct
     8161 acgctggcat atatgtatcg cttctactac cgcatcattt ccaacgccaa tgtgctcatc
     8221 aacggcattg agaaagtacc cggcccacaa gccgacaaag acatgatcaa aggccaggcg
     8281 ctggcatatc gcggctggtc gcacttccag ttggtacagc tttggggcga gcgttatgac
     8341 ggctccagaa ccaacagcca gttgggcgtg ccgattttgt tggaaaacac attagtgggc
     8401 cagccccgcg ctacggtaga ggaggtttat acccaaatca acaaagattt ggatgaagcc
     8461 atcacgctgt tggccggtta caaccgcacc ggatcggctg ccaaatccaa catcaacgtg
     8521 aatgtggctc agggtttcaa agcccgcgta gcgctgaccc agcaaaaatg ggccgatgct
     8581 gccgccgccg ccgccgccgc tcgcgccggc gtctccctga tgagcaacgc gcaatacacg
     8641 accggcttca acgacatcac caatcctgaa tggatgtggg gcagccgtca gatttccgac
     8701 cacaacacct tcttctggtc gtacttcgct tatatgtcgg ccaacttcaa ctctacggtg
     8761 atccgtacgc agc
//
LOCUS       DAORLF010000178         8753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4344_length_8753_cov_1.805473, whole genome shotgun sequence.
ACCESSION   DAORLF010000178 DAORLF010000000
VERSION     DAORLF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_8753_cov_1.805473"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK198_14265"
     CDS             <1..611
                     /locus_tag="PK198_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39950.1"
                     /translation="PPSPDVAVFPLKTQRFSVTAVVPELKTPPPLVPAVLLEKVQSLI
                     VREVERLNIPPPELTEEFPEKKQAERMGEPAEMYAPPPLEAVLLEKVQLFKVGEDELL
                     FIPAPAPEEFPEKRQAEKTGELPNELYVPPPLPPLPVELLENVQLLTVGEDDLLYKPP
                     PTLRRKDSNSMVMLDEPSAFPFSTVKPSNTVEFASLPDKTTW"
     assembly_gap    633..658
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<659..2448)
                     /locus_tag="PK198_14270"
     CDS             complement(<659..2448)
                     /locus_tag="PK198_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39951.1"
                     /translation="MKTSNTSSPQQARLYTANILHHFPLHFFFAGAFAWLLWSGAAPG
                     SSAETMLAITVQPTDQYDCTSNMASFSITATGASSYQWFRSSDEGANWIPLGNGDYGL
                     LAGVNTPTLTASGGSLVLINGLLFRCQVSDGSTTLNSAAARFQIGLPATSIQYGHTGY
                     CTGAGPVAVVLTGAQGGTFTASPAGLSIHAANGQITPGSSTPNTYTVTYHVPAQHGCA
                     AINATTLVSISTNVQPVISYGAAPLCHHETSVPVSLSGAGGGTFSSSAGLSVNSATGA
                     INPSASTPGTYTVTYTTSGVAATGCTAYMATTNVTINNQLNATITGGGNHNCAGGSYN
                     LTVTGTPNSIVTYKINGGDDLTLTIGGSGTTVLNTGSLRENTSYQLVSVRFADTEFCA
                     RPLSSTANLTFNRPYLTYAVSAPATANEGEVVNIDITNRPFTIILYQINGGLVQSALI
                     NNSTGLISIPVTVNGTTTFTRFSSSYQSSPTCSTTYPDSAAVVISVAACPPVGTIWYV
                     DASAAPGGTGSSWTCAFQNLQDAINAASSGDEIWVKAGTYKPTAYPVGCSGCSTARDY
                     TFHLKDGVAIYGGFAGMETARTQRDWVANPT"
     gene            2605..>3827
                     /locus_tag="PK198_14275"
     CDS             2605..>3827
                     /locus_tag="PK198_14275"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF39952.1"
                     /translation="MQRYAILLTFLLAAAGAPAQTLADSLRNALSRQQGDQRYHTLQQ
                     LCNIYVRPTLTEEELLGYARELIDLAALKKDTAAWVNGYVSMTLKEKRTKENTPSQYL
                     LKAKDLSQNHPELLAEIFFWENEGYEGLGLETEAMTAIAKGLALYDRHKLPPLFRIKL
                     LGAAARIYSIQNDIPATDSLLNAMVSYVQTPTDSMEALRYVASCCENIGQLDKALEAY
                     LKSYRIAHRIHNLLFAVTNLRQAASILRDQKRYDKALKYYEQAASLAKANNNISQLGS
                     IYHSMGMLHHRSGNHEEAIRYGQLALDIKQDQGRPRKILTTAAMMAESYRAMERYEQL
                     TAICMEYLPLSQSVNMHEVTSKMGYMAAIAQAKTGRPDEGRQWLEPANQAALEVKNLE
                     ELVPVLYLAYDAHASL"
     assembly_gap    3828..3996
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3997..4795
                     /locus_tag="PK198_14280"
     CDS             <3997..4795
                     /locus_tag="PK198_14280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF39953.1"
                     /translation="AISMAQLTAARTRQWALIIGLLSLSVIAFAFYRNAVLRKRNNRA
                     LQQANTELQRKNHEVQTLLREIHHRVKNNLQIVSSLLRLQARKTDDAGAIEALRTSQA
                     RVRAMALLHQRLYQESNLVGIPMQSYMEELSRSLFDTYRVDEDRIQLHTDLEDVTLDV
                     DAAVPIGLITNELITNALKHAFPNSRGGIIQIILKTDADQLRLEVTDNGVGTPLQNGK
                     PQTSGPSFGLELVETLAEKLQATLHFSNQQGSRCTLVAPLSISIAAA"
     gene            4792..5523
                     /locus_tag="PK198_14285"
     CDS             4792..5523
                     /locus_tag="PK198_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF39954.1"
                     /translation="MIRILIVEDEPLIAKDLEYAVQDLGYSISASCRNSRQALEALQR
                     EVPDLIICDIHLHNDDWDGIRLAQEIRLRYDIPLIFLTALSDAETIRRAAQTQPQAYL
                     TKPFDERTLYAAIELAIVRFASPEQASASPAEAPPPFIAGSFFVKDKKRLVKIKAEDI
                     QWIKADGAYSLLAANQQQYLLSVHLGAIESSLGAFSFARVHRSYLVNVQHIEAIEDDL
                     IFIGKERIPIGKSFREDFYSRIRQL"
     gene            complement(5544..>6267)
                     /locus_tag="PK198_14290"
     CDS             complement(5544..>6267)
                     /locus_tag="PK198_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF39955.1"
                     /translation="LFRFSYNKIKHAGINIFFTLTTIVINFLMAFILLSSADWAVANK
                     FGILQWLPDMPLWAFAIVGLLLLDLIGAWFVHWAEHKVRWMWRFHLIHHTDTTVDTTS
                     ANRHHPGESVFRFVFTTMGVVLAGTPIWIVFMYQSMSVLLSQFNHANIRLPQWLDDAL
                     SWVIISPDMHKVHHHYKLPYTDSNYGNIFSFWDRLFGTYMKLDREHIVYGIDTHYKEE
                     EHSRLGNLLKIPFQKYRKPTSE"
     assembly_gap    6268..6448
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6529..>7656)
                     /gene="sppA"
                     /locus_tag="PK198_14295"
     CDS             complement(6529..>7656)
                     /gene="sppA"
                     /locus_tag="PK198_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRF39956.1"
                     /translation="DAFEEFLSDISEGRNIPVSELRRIADGYLAFMPEQALQLKMVDE
                     LAHREAALEGIRKKLGLGKKEKIKTISIEDYNLSNPAKSNLKASNKIAVVYAEGNIVD
                     GKGEPGSIGGAKYVDIISKIRKDDKVKAIVLRINSGGGSAMASEDILRELELAQEQGI
                     KVVVSMGDYAASGGYYIACKADSIFAEPNTLTGSIGVFSMIPSAQKLLNDKIGITFDT
                     VKTGPFAHGISPFYDMSEQEGKIMQAFVDNLYTTFIGHVSKGRNLSPEAVNEIAQGRV
                     WTGKKALGIGLVDRLADLDEAILSAAALADLKDYRITEYPRIKEPIQQFIDELTGTTE
                     TRTQRALRSQLGSMYPHYRFVKELQDSKGAQARLPFIIPFR"
     assembly_gap    7657..7760
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7761..>8151)
                     /locus_tag="PK198_14300"
     CDS             complement(<7761..>8151)
                     /locus_tag="PK198_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRF39957.1"
                     /translation="LYDAPPIPEKTNNLPLDPFSLQEQKILGTHEILDALQRARKDDH
                     IKGVFLETDGLQGGLATADLIRDALAEFQKEGKFVYAHSNAYTQGSYYLASAADKIYL
                     SPVGLLDFRGFAAQVPFFKDMLDKAGVH"
     assembly_gap    8152..8168
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8465..8518
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8519..>8753
                     /locus_tag="PK198_14305"
     CDS             <8519..>8753
                     /locus_tag="PK198_14305"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF39958.1"
                     /translation="ICWTALHAQVLEVTNAPPITPQNLITNIFLGDGVQVLSVNYEGP
                     NMAVGFFKEGQTAVGMDRGLVMTTGRAVTQGLNF"
BASE COUNT         1946 a         2175 c         2227 g         1854 t
ORIGIN      
        1 cgccgccgtc gcccgatgtc gcagtgtttc ctttgaaaac gcagcggttc agcgtgacgg
       61 cagtagtgcc tgagttgaaa acaccgccgc cgttggtgcc ggctgtgttg ctcgaaaaag
      121 tacaatcgtt gattgttagg gaagtcgaaa gattgaacat ccccccaccg gagttgacgg
      181 aggaatttcc agaaaaaaaa caggccgaga gaatgggaga acccgccgaa atgtatgctc
      241 cgccgccact cgaagctgtg ttacttgaaa aggtgcagtt gttcaaggtt ggggaggacg
      301 agttactgtt cataccagct ccagcaccgg aggaatttcc tgaaaaaaga caagcggaaa
      361 aaacgggcga actaccaaac gagttatatg ttccaccgcc attgccgccg ctgcccgtcg
      421 agttgttgga aaatgtgcaa ttattgactg tcggggagga cgacctatta tacaagccgc
      481 caccaacgct ccgcaggaag gatagtaact cgatggtgat gctcgacgag ccgtcggcat
      541 ttcctttttc aacggtgaag ccatccaata cggtcgagtt cgcatcgctg cccgacaaaa
      601 cgacgtggtg agcattgtcc gagttgtcgc cgnnnnnnnn nnnnnnnnnn nnnnnnnngt
      661 ggggttggct acccaatccc gttgggttcg agcggtttcc atacctgcga agccgccgta
      721 aattgctacg ccgtctttca aatgaaaagt gtaatctctg gccgtactac aacctgaaca
      781 gccaacagga tatgcagtgg gcttgtaagt gcccgccttc acccatatct catccccgct
      841 gcttgctgca ttgatggcat cctgtaagtt ttggaaagcg caagtccagg aactgcccgt
      901 gccgccgggg gctgctgagg cgtccacata ccagatggtt cccacaggcg gacacgcagc
      961 taccgaaatc actaccgccg cagaatccgg gtaggttgtg ctgcaggtag gactgctttg
     1021 ataagaagag ctgaagcggg taaaagtagt tgttccgttg acggtaaccg gaatcgagat
     1081 gagtccggtg ctgttgttga tgagggccga ttgcaccaag ccgccgttga tttggtataa
     1141 aataatggtg aacggacgat tcgtgatgtc aatgttgact acctctcctt catttgcagt
     1201 agcgggggcc gaaacggcat aggtcaggta ggggcggttg aatgtcaggt tggcggttga
     1261 gctgaggggc cgtgcgcaaa attcagtatc tgcgaagcgt acagatacca attggtaaga
     1321 ggtgttctcc cgaagcgatc cggtattcag caccgttgta cccgaaccgc cgattgtcag
     1381 ggtgagatca tctccaccat ttattttgta agtcactatg ctgttgggag tacccgttac
     1441 ggtcaggttg tacgaaccgc ccgcacaatt atgattgccg ccgcctgtga tggtcgcatt
     1501 gagttgattg ttgatggtaa cattagtagt agccatatat gccgtacagc cggtagctgc
     1561 caccccgctt gtagtgtatg tcacggtata ggtgccgggg gtggaggcag acggattgat
     1621 ggcgccggta gcgctgttga cagacagccc cgccgacgag gaaaacgtgc cgccgcctgc
     1681 tccgctcagg cttacaggaa cagacgtttc gtgatgacac aacggagcag cgccataaga
     1741 aatgacaggc tgcacattcg ttgaaatgct cacaagggtc gttgcattga tggccgcaca
     1801 gccatgttgg gccggtacat ggtaagtgac ggtgtaggtg ttgggcgtcg agctgccggg
     1861 ggtaatctgc ccgttggcgg catggatgga aagtcccgca ggcgatgccg taaatgtgcc
     1921 gccttgcgcc cctgtcagca ctactgccac cgggcctgcg cccgtgcaat agcctgtatg
     1981 cccgtattga atgctggtag ccggcaaacc gatttggaaa cgagccgctg cggaattgag
     2041 cgttgtggaa ccatccgaca cctgacaacg aaacaacagt ccgttgatta agactaagct
     2101 gccccccgat gcggtcagcg taggggtatt tacgccggcg agcaagccat aatctccatt
     2161 gcctaatggt atccaattgg cgccttcatc cgagctgcgg aaccactgat agctgctggc
     2221 ccccgttgcc gtaatcgaaa aagaggccat attggaagta cagtcatatt ggtcggtggg
     2281 ctgtaccgtg atggcaagca tggtttcggc actgctgccc ggggccgccc ccgaccacaa
     2341 gagccaggca aacgcgccgg cgaagaaaaa gtggaggggg aaatggtgta aaatgttggc
     2401 tgtgtacagc cttgcctgtt gcgggctgct cgtattggat gttttcatgt cgaagtggat
     2461 tttgttcaaa tactccgaaa tgtacatgca cagaataggc tgttgggccg ggcaattaac
     2521 gaacgggctt gttttcttaa cgaaggcgct gttttggcca atgaaaattt tcgtcgtatc
     2581 attgcatcca catacaacct gcccatgcaa cggtatgcca tcctccttac ttttttactc
     2641 gccgccgccg gggcgcctgc ccaaaccctg gcggacagcc tccgcaatgc cttgtcgcgg
     2701 cagcagggag accaacgcta ccacaccttg cagcaacttt gcaatattta tgtacgcccc
     2761 actctgaccg aggaagaact gttgggctat gcccgggaac tgatagactt ggctgccctg
     2821 aaaaaagaca ccgctgcatg ggtgaacggc tatgtaagca tgactctcaa agagaaacga
     2881 acaaaagaaa atacccccag ccaatatctg ctgaaagcca aggacttatc gcaaaaccac
     2941 cccgaactct tagccgaaat ctttttttgg gaaaacgagg gctacgaggg gcttggtttg
     3001 gaaactgaag ccatgacagc cattgctaaa ggtctggcac tgtacgaccg gcataagctg
     3061 cctcctcttt ttcgcatcaa actgctgggc gccgccgcac gtatttacag catccaaaac
     3121 gacatccccg ccacagactc attgctgaac gcgatggtga gttatgttca aaccccgacc
     3181 gactccatgg aggcactccg gtatgtggct tcttgttgtg aaaacatagg ccagttggac
     3241 aaagcattgg aggcctatct caagtcctat cgcatcgcac atcgtattca caatctgctt
     3301 tttgccgtca ccaacctccg gcaggctgcc tccatcttac gcgaccaaaa aaggtatgac
     3361 aaggcgctga agtattatga acaagcggct tctttggcca aggccaacaa caacatctcg
     3421 caactcggca gcatctatca ttctatgggg atgctccatc atagatcggg caatcatgag
     3481 gaggccatac gatacggtca attggctctt gatattaagc aggaccaagg acgccccaga
     3541 aagatactca ccactgctgc catgatggcg gagtcgtatc gcgctatgga gcgatacgag
     3601 cagttgactg ctatttgcat ggagtacctg cccctgagcc aatcggtcaa tatgcacgaa
     3661 gtgaccagta aaatggggta tatggctgcc attgcccaag ccaaaacggg cagacccgac
     3721 gagggacgcc aatggcttga acctgccaat caagccgctt tagaggtgaa aaatctggag
     3781 gaattggtcc ccgtgttata cctggcatac gacgcccatg cctctctnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc atcagtatgg cacagttgac
     4021 ggcggcccgc acccgccaat gggcgctcat catcgggctg ctatcgctga gcgtaatcgc
     4081 ttttgccttt tacagaaatg ccgttctccg aaaacgaaac aaccgcgccc tgcaacaagc
     4141 caataccgag cttcagcgca aaaaccacga ggtacaaacg ctcctccgcg aaatccatca
     4201 ccgcgtaaaa aacaacctgc aaatcgtatc cagcctcctc cgcctccagg cccgcaaaac
     4261 cgacgatgcc ggcgccattg aggccctccg caccagtcag gcccgcgtgc gcgctatggc
     4321 cctgctgcac cagcgccttt atcaggaaag caaccttgtg ggcattccca tgcagtctta
     4381 catggaggag ctttcccgca gcctcttcga cacctatcgc gtggacgaag accgcatcca
     4441 actccatacc gatttggagg acgtgacctt agacgtggac gccgccgtgc ccatcggcct
     4501 cattaccaat gaactcatta ccaatgcgct gaaacatgct tttccaaaca gccggggcgg
     4561 catcattcaa atcatcctca aaaccgatgc agatcaactc cgcctcgaag tgaccgacaa
     4621 cggtgtgggt acgcccctcc aaaacgggaa acctcaaacg agcggcccct ccttcggcct
     4681 cgaattagtg gaaaccctcg ccgaaaaact tcaggccacc ctccatttct ccaatcagca
     4741 gggcagccgc tgcacgctcg tggccccgct ttccatttca attgccgccg catgatccgc
     4801 attctgatcg tagaagatga accgctcatt gccaaagact tagagtatgc cgtgcaagac
     4861 ctcggctact cgatttctgc gtcttgccgc aactcccgcc aggcattgga ggccctccaa
     4921 cgcgaagtgc ccgaccttat catctgcgac atccacctgc acaacgacga ctgggacggc
     4981 atccgcttag ctcaggaaat ccgccttcgc tacgacatcc cgctcatttt cctcaccgct
     5041 ctctccgacg cagaaaccat ccgccgcgcc gcccaaacac agccccaggc atacctcacc
     5101 aagcccttcg acgagcgcac cctctatgcc gccatcgaat tggccatcgt tcgttttgcc
     5161 tcgcccgaac aagcaagcgc ctcgcccgcc gaagccccgc cgcccttcat cgccggcagc
     5221 ttcttcgtca aagacaaaaa gcgattggtc aaaatcaaag ccgaagacat tcaatggatt
     5281 aaagcagacg gggcctactc gctgctggcc gccaatcagc aacaatacct gctcagcgtt
     5341 catctcggcg ccatagaaag cagcctcggc gccttctcct tcgcccgcgt acaccgctcc
     5401 tatttggtca atgtgcagca catcgaagcc atagaagacg acctcatttt tattggaaaa
     5461 gagcgcattc ccatcggcaa gagtttccgg gaagattttt acagccgcat caggcagctc
     5521 tgatttttca gtcgtcccct cccctactcg ctcgtgggtt tgcggtattt ctggaatggg
     5581 attttgagca aattgcctaa gcgcgagtgt tcctcctctt tgtagtgggt atcaatgcca
     5641 tacacgatgt gctcgcggtc caacttcata taagtgccga acaagcgatc ccaaaaggag
     5701 aagatattgc cgtagttgga gtctgtgtag ggcagtttat agtggtggtg taccttgtgc
     5761 atgtcgggcg aaataatgac ccaactcagg gcatcatcca gccattgcgg caagcggata
     5821 ttggcatggt tgaactgcga aagcaacacc gacatagact gatacatgaa tacgatccag
     5881 atgggcgtgc ccgccaatac tacccccatg gtggtgaaaa cgaaacgaaa cacgctctcg
     5941 ccgggatggt ggcgattggc cgaggtcgtg tccacggttg tatcggtatg atgaatgagg
     6001 tgaaaacgcc acatccagcg cactttgtgt tcggcccagt gaacaaacca ggctccgatg
     6061 agatcgagca gaagcaggcc cacaatggca aaagcccaca gcggcatatc ggggagccat
     6121 tgcaagatgc cgaacttatt ggctactgcc cagtctgccg aacttagtaa aataaaggcc
     6181 atcagaaaat tgatgacaat ggtggtgagg gtaaaaaaga tgttgatgcc ggcgtgtttg
     6241 attttgttgt aagaaaatcg aaacaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ccggcgccaa cctcaacttc gaccgtctgc
     6481 gcttcgacaa gctaacggct ggcggctaac agctaacagc caatcgtact acctgaatgg
     6541 aatgataaaa ggcagacgag cctgagcgcc tttgctgtct tgcaattcct tgacgaagcg
     6601 atagtgcgga tacatgctgc cgagctgtga gcggagggcg cgttgagtgc gcgtttcggt
     6661 agtgccggta agttcgtcta tgaactgctg gatcggctct ttgatgcgcg gatattcggt
     6721 gatgcggtag tctttaagat cggcgagggc tgcggcggag aggatggctt cgtcgaggtc
     6781 ggcgagacgg tccacgaggc cgataccgag ggcttttttg cccgtccaga cgcggccctg
     6841 ggcaatttca ttcacagctt cgggcgatag gttgcggcct ttagagacgt ggccgatgaa
     6901 ggtggtgtag aggttgtcca caaaagcctg cataattttg ccttcctgtt ccgacatatc
     6961 gtagaacggt gaaatgccgt gcgcaaaggg gccggttttg accgtgtcga aggtgatgcc
     7021 gattttgtcg ttgaggagtt tttgtgcgct gggaatcatg ctgaaaacac caatggaacc
     7081 tgtgagcgtg ttgggttcgg cgaagatgga gtcggctttg caggcgatgt agtagccgcc
     7141 ggatgccgcg tagtcgccca tagaaacgac gaccttaatg ccctgttcct gagccagttc
     7201 gagttctcgg aggatgtctt cggaagccat ggcgctgccg ccgccggagt tgatgcgaag
     7261 gacgatggct ttgactttgt cgtctttgcg gatttttgaa atgatgtcca catatttggc
     7321 gccgccgatg ctgccgggtt cgcctttgcc gtccacaata ttgccctctg catacaccac
     7381 ggcgatttta ttggaggctt tgagattgga tttcgccgga ttgctgaggt tgtagtcctc
     7441 aatggagatg gttttgattt tttctttttt gccgaggccg agttttttgc gaatgccttc
     7501 gagggcagct tcgcggtggg ccagttcgtc caccattttg agttggaggg cctgttcggg
     7561 catgaaagcg aggtagccgt cggcgatgcg gcggagttcg gaaaccggga tgttgcgacc
     7621 ttcagatata tcggagagga actcctcgaa agcatcnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn gtggactccg gccttgtcga gcatgtcttt aaaaaacggc
     7801 acttgcgctg cgaagcccct aaaatcgagc aaacccaccg ggctgaggta aattttgtcg
     7861 gcggcagatg ccagatagta gctgccttgc gtgtaggcat tggaatgggc atacacgaat
     7921 tttccttctt tttggaactc ggccagcgca tcgcgaatga ggtcggcagt agcgaggccg
     7981 ccctgtaggc cgtcggtttc cagaaacacg cctttgatgt ggtcatcttt ccgagcgcgt
     8041 tgcagcgcat ccagaatttc gtgtgtgccc agtattttct gctcttggag gctaaaagga
     8101 tcgaggggca ggttgttggt tttttcggga atgggtggtg catcatagag gnnnnnnnnn
     8161 nnnnnnnnat acgctgttgg gttctatttt tttgcctttg ctttccatag agccggcaac
     8221 agcgccgaga attccgaaag aaatgatgcc cgtgacgatg gtggccaaaa tgacgccgag
     8281 gcaagaggcg aaaacgaatt taaagaattg tcccatgatt gatttttttg ctgttttcaa
     8341 cagtcaaagg tagaaatcag actcgcgttg tgtcctgatt ttagataaaa accgaaaaaa
     8401 gggcgacaat ctttcgacta agcgggtacc ttgcaacgga aagcactact tttgaacgaa
     8461 aattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     8521 tttgctggac ggccttacat gctcaggttt tggaagtaac caatgcgccg cccatcacgc
     8581 cacagaacct cattaccaat atttttctgg gcgacggcgt gcaagtactc agcgtcaatt
     8641 acgaagggcc gaatatggca gtcggctttt ttaaagaagg ccagacagcc gttggtatgg
     8701 acaggggctt ggtgatgacc accggacggg ccgtaacgca ggggttgaat ttt
//
LOCUS       DAORLF010000179         8752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4346_length_8752_cov_2.170634, whole genome shotgun sequence.
ACCESSION   DAORLF010000179 DAORLF010000000
VERSION     DAORLF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_8752_cov_2.170634"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PK198_14310"
     CDS             complement(<1..467)
                     /locus_tag="PK198_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRF39959.1"
                     /translation="MEYRIERDTMGEVKVPADKYWGAQTERSRNNFKIGPEGSMPKEI
                     IYAFAYLKKAAAWANHDLGVLATEKRDLIGIVCDEILEGKLDAEFPLVIWQTGSGTQS
                     NMNVNEVIAYRAHVLSGGKLTDEKKVLHPNDDVNKSQSSNDTFPTAMHIAAYK"
     gene            complement(540..1625)
                     /locus_tag="PK198_14315"
     CDS             complement(540..1625)
                     /locus_tag="PK198_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF39960.1"
                     /translation="MPANIQHFESVVIQIATPYTTGTGFLLPQFGLIVTNEHVIRDNR
                     DAVIEGANVQRQVVRVLFCDIRLDIAFLEAKDLPEVPSVQIKTDAAPQTGERVYAVGH
                     PFGLRFTTTSGIISNPEHHSDGIVFIQHDAALNPGNSGGPLIDDEGNIIGINTFIIQN
                     GQNIGFSLPIRYVADAALKYQEKHGVPAAACYGCGSIVFDTGKAMKYCPNCGAAITLP
                     NYAEPYEPIGMAATIEQLITATGHDVHVSRRGPNAWELQHGSAKIALYYYEKTGLITG
                     EAYLCSLPGQNIKEVYQYLLHENYHLAGLTLSVRQQDIILSLLIYDRYLNLETGLRLL
                     EQLFTQADAYDNILVEQFGAAWRHPNE"
     gene            complement(1646..1993)
                     /locus_tag="PK198_14320"
     CDS             complement(1646..1993)
                     /locus_tag="PK198_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39961.1"
                     /translation="MTKRLRLTGFARFLFVMVILVPAAYIGASYYNGEDGIQNIKNLL
                     GIEAASSGNKAPSQRKSLDTPANSSGVTEELEQRITDLEAENRELKVQIRDLKLELKA
                     TQLQLKNTKSNAK"
     gene            2640..3488
                     /locus_tag="PK198_14325"
     CDS             2640..3488
                     /locus_tag="PK198_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRF39962.1"
                     /translation="MKSNYYESNYHRRGDSEGNVSNNKRNSPQHHGGFEQPEDITFSM
                     LFQRFAGGLRLLWTALKYRLMKLLGITGAKSKSSWRFSLIKFTMLGIVIILVTQKDIR
                     FSVNLKAPLAGLGQSDEPQAVAASNKALGKFSLSDALPFGKSASNDEVIAVSDLNADE
                     VNDYIGRFSKVAVAEMRKFGIPASIKMAQAILESHAGQSLAVRETSNHFGKPLQNQEY
                     VSAWENWRAHSLLLKSEYSALFEDAYGYKQWAKGLQKLKYNTDKSYADKLIEVIEKYD
                     LTLLDE"
     gene            complement(3469..4428)
                     /locus_tag="PK198_14330"
     CDS             complement(3469..4428)
                     /locus_tag="PK198_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRF39963.1"
                     /translation="MSEIRSNAVATALKGMAMGVAEVIPGVSGGTIAFITGIYERLLN
                     AIKSVLGPGIFKAFKSGGIKEAWRFIDGNFLIALMAGMAAGVVVGVFGISHLLEHYPQ
                     LVWSFFFVLILASARLLFRQVGNWNLKTIAALVITSIAAFYYTIAAPSEGIQEYWFVF
                     VSGAIAISALMLPGISGSFILVLLGMYSYILGSVKKLLTDFDFGSGLVAVVFAMGCLV
                     GLATFSRVLTWTFKHHYHITMAALTGFMIGSLNKIWPWKEVVSWRTDSHGEQVALLEK
                     SVLPAQFDGDPMVAACILLAVAGFVTVSVIEKIGKQTTTHPAA"
     gene            complement(4455..5990)
                     /locus_tag="PK198_14335"
     CDS             complement(4455..5990)
                     /locus_tag="PK198_14335"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRF39964.1"
                     /translation="MKSFNIKKGLPVLLFIGIIVALNVLIQPVLLRLDLTKNKQYTLS
                     AASKAVLGNLKENVTITAYFSEGLPPDVGRVRRDFQDLLVELSSVAHGKLDYKFVAPV
                     TDAVKQEAARYGIQPLMINVREKDQVKQQQDFLGAVIKKGAETEVIPYVQPGTSMEYN
                     LVAAIKKLSHQNKPKIAFIQGHGEASLNELSQVARGLSQLYEIEQLDMNSSTLSADSI
                     KVAIVAGPKTPFAPEHFQQLENYMQSGGSLVIAFNRMEIDMQSGMAFPSQTGLEYWLE
                     SKGLKVDTSLVLDVQCGSVTVPQYMGAIQINTQVQFPYLPLAVTFADHPAVKGMQQVM
                     FPYVSPLEFAGNQANTAFTPLVFSSDKSGLALSPVTLSVGEKKWTQADFPLGNQVLAG
                     LIEQKNADGSVSKLIVFGDADFALSNEQGRGVSEENINLMVNAIDWLGDDSGLMNLRN
                     KAAAIRPIEAKYLMGDSSKSRELIKWANVLVPVLLLSLVGIFLHQKNKRLRNKRSAET
                     YES"
     gene            complement(5987..6715)
                     /locus_tag="PK198_14340"
     CDS             complement(5987..6715)
                     /locus_tag="PK198_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF39965.1"
                     /translation="MRTLNPAWIIARRELRFNFDSLIAYLLISGFLLFTGGFTWLNQA
                     SNIFAIKQADLGIFFSVAKWTLFFFIPAMTMRLLAEERKNGTFELLLTKPVSHAQIVA
                     GKFLSSWILVGITLALTLPYYFSVAYVGEIDHGATLTGYLGLMLMSAAYIAIGLLAGS
                     FTDNQIVALLLSLFIGVFFQFLFETIGAFLPGALGEGFYYLSMNKHLDSLSRGVVDVK
                     DVFYFASVTLIALLLTIRNLTAGK"
     gene            complement(6768..8204)
                     /locus_tag="PK198_14345"
     CDS             complement(6768..8204)
                     /locus_tag="PK198_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39966.1"
                     /translation="MRKDRSFDDIIKDKLNKVTPAYQPENWERFRERLDAEHQDEVQD
                     MEFFDRLVRQKTPSVSNAGLSNNWTLVNRLDLIYRREREIAISKVLELMALSLLLWIV
                     DAHIITQNTGIVFASNEFQNTRHTAQDRVNNTLENKYSDSDSQQTELERLAQQASRAS
                     DMENTSSGQESFLLPTQIEESVNQFIADQYLWELSSYLVPGVQVHLPKPIEYNLNAPT
                     FDATAPAVVAEILPKSKSKKFTISMFGSTDINSIYTPGSVEGPFSELDRTTFTIPALQ
                     RYSLGYSGGAALAIEKGRWETEIGMLYTAKIYKARPVLYVTGSVSEGFSAEGLKDIEL
                     NMVSVPFQLKYNWLDKNGWRIYGGLGASVNVVLESSYLLTDEDAFHSSSFNLRLPNPE
                     PTGVPKSRGLQSKNLDGGWLQGGGLANNSYITGSVSAGIDRRFSSGWSVFVQPTFQHS
                     LYYFSKGIGPDKDQIHTFSIVSGIRVRL"
     gene            complement(8191..8748)
                     /locus_tag="PK198_14350"
     CDS             complement(8191..8748)
                     /locus_tag="PK198_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF39967.1"
                     /translation="MPIGYEEQDFIKACVRRETWAQKLIYEEFYGTMMALCLRYAGNE
                     EDAMDILHEGFIKVFRNIHKYQPDTSLPAWIRRIMVNTAIDFYRKTVRRRTEDIDAAF
                     DLSSDEADAISKCSEQQILEAIQLLTPVYRAVFNLFVLEGYSHREIGELLSITESTSR
                     SNLVKARAKLKEILTSRKFGYHEEG"
BASE COUNT         2336 a         2125 c         2020 g         2271 t
ORIGIN      
        1 aatttgtagg cggcaatgtg catagcggta gggaaagtat cattcgagga ctggcttttg
       61 ttcacgtcgt cgttggggtg cagcaccttt ttttcatccg tcaatttgcc cccgctcaac
      121 acatgggcgc ggtaggcaat cacttcattg acgttcatgt tgctttgcgt accggagccg
      181 gtttgccaga ttaccaacgg aaattcggcg tccaacttgc cttccaggat ttcatcacac
      241 acaatgccga tgagatcgcg cttttcagta gccaaaacgc cgaggtcatg attggcccag
      301 gcagccgctt ttttcagata cgcaaaagca tagatgattt ccttcggcat actgccttcc
      361 ggcccgattt tgaagttgtt gcgggagcgt tccgtttgtg cgccccaata tttatcggca
      421 ggtactttca cctcgcccat agtgtctctt tcgatgcggt attccataaa aaagtattgt
      481 ttttagttgt tccgcaaagg tattgaggcc gggcgaaatc cggcagctat tgtgctgaat
      541 tattcgttgg gatgcctcca cgcagctcca aattgttcca ccaatatgtt gtcataagca
      601 tcggcctgcg tgaatagctg ctcaaggagc cgaagaccgg tttccaaatt gagataacga
      661 tcataaatga gcaacgacag tataatgtct tgttgccgta cgctcagcgt aagacccgcc
      721 aaatgataat tctcgtgcag caggtattga tacacctctt tgatgttttg gcccggcaaa
      781 ctacacagat aagcctcgcc ggttatcagc cccgtttttt cgtagtaata caaagcaatt
      841 ttggcactcc cgtgttgcaa ttcccaggca ttggggcctc gacgggaaac atgcacatca
      901 tggccggtag ctgttattaa ttgctcaatc gttgcggcca ttcctatcgg ttcgtaaggt
      961 tcagcataat tgggcaatgt gatcgcagcc ccgcagttag ggcagtattt catggccttt
     1021 ccggtatcaa acacaatgga accgcatccg taacaggcag cagcaggaac accgtgcttt
     1081 tcctgatatt tcaaagccgc atccgccaca tatcgaatcg gaagggagaa gccaatgttt
     1141 tgaccatttt gaataatgaa tgtgttgatg ccgatgatgt tgccttcatc atcaatcaat
     1201 ggcccgccgc tgttgcccgg attgagcgca gcatcgtgct gaatgaacac aattccatcg
     1261 gagtgatgct ccgggttgga aatgatgccg ctggtggtag tgaaacgcaa cccgaaagga
     1321 tggccgactg cataaacacg ctcgccggtt tggggcgctg catccgtttt aatttgcacg
     1381 cttggcactt cgggcaagtc ctttgcttcc aaaaacgcta tgtctaacct gatatcgcaa
     1441 aaaagaaccc gaactacctg ccgttgaaca ttggcccctt caatgactgc atctctgttg
     1501 tctctgatga cgtgttcatt ggtgacgata agcccgaatt gaggcaacaa aaagccggtg
     1561 ccggtcgtgt aaggcgtggc aatttggata acaaccgatt cgaagtgttg gatgttggcc
     1621 ggcatgttca gaaatggctc aagaattact ttgcattgga ctttgtgttc ttcaactgta
     1681 gttgagtagc tttcaactca agtttcaggt cccggatttg aactttcaac tctctgttct
     1741 ccgcctccaa atcggtaatt cgttgctcta attcttctgt cacgcccgaa ctgttggcag
     1801 gcgtgtccaa cgatttgcgt tgggaaggcg ccttattccc tgatgaagcg gcttctatgc
     1861 ccaacaggtt tttgatgttc tgaatcccat cctcaccatt atagtaggag gccccgatgt
     1921 aagcagcggg caccagaatc accattacaa aaaggaaacg ggcaaacccg gtaagtctca
     1981 gtcgttttgt cattgtggta agaaattgct gtggtaaagt tagtcaagtt ggcccaaaca
     2041 gatggatttt tctgctgaac gttcttatat gtcagaccag aaaaaaaaac tcctgcttca
     2101 gggaggcaaa aaaaatgaag caggagccaa caataataaa catactatga gaaaactaca
     2161 ctgctgcaaa agtagcacgg ctcctttgtg cagcatagct gtatttatca gaagagatgg
     2221 tattcagaag cagcggtgta tctggtttag gcagcgcttg tttagtggga gcaggtgagg
     2281 taggggcaaa aaaaaaccta cacccgaagg gactgagtgt aggactatta aacatactat
     2341 gagaaaactg atgcaaagat aaaggaggcg tacaggttca aaagttacat ttatggaacg
     2401 gtatcaaaat ggcacatttt cgagtcatta gccgagaata cggtgcaatt ttccgggtga
     2461 acggtcgcat ttgcgaaaaa acgtgccaaa accatgctac tcccggctct ttttgttatt
     2521 cgtacacgag agaaactttt taaaataatt tgttttaaac tattgtttta aaaacaaaaa
     2581 caccttacct ttgtcttgct ttcgcatcaa ctttgtaacg accaacaaat attgcagtca
     2641 tgaaatcgaa ttactacgaa tccaattacc accgaagagg agattcggag ggcaatgttt
     2701 ccaacaacaa gaggaattcg cctcaacatc atggcgggtt tgaacaacct gaagacatta
     2761 cgttcagcat gttgtttcaa cgttttgccg gtggactgag acttctgtgg acggctttaa
     2821 agtacaggct gatgaagctg ctcggcatta cgggcgccaa atcaaaatct tcgtggaggt
     2881 tttcactcat caaattcacg atgttgggga tagttatcat tttggtgacc caaaaggaca
     2941 tacgcttctc cgtcaacctg aaagccccct tagccggcct tggtcaatca gatgaacccc
     3001 aggcagtagc agcgtccaac aaggcgctcg gtaaattcag cctgagcgat gcgctgccat
     3061 ttggcaagag cgcctccaac gacgaggtca ttgccgtttc cgacttaaac gccgatgaag
     3121 tgaatgacta cataggccgc ttttcaaaag tagcggttgc ggaaatgcga aaattcggca
     3181 tacctgcttc catcaaaatg gctcaagcga tattggaaag ccatgcgggt caaagccttg
     3241 cagtgcggga aaccagcaac catttcggca agccgctaca aaatcaagaa tatgtgagcg
     3301 cctgggaaaa ctggagagca catagtttgc tcctcaaaag cgaatactcc gccctgtttg
     3361 aggatgccta cggctacaag caatgggcca aaggattgca aaaacttaaa tataataccg
     3421 acaaaagcta cgccgacaag ctcattgaag tcattgagaa atacgatctc acgctgctgg
     3481 atgagtagtt gtttgttttc caattttttc aataacagaa accgtcacaa acccggcaac
     3541 ggccagtaaa atgcaggcag ctaccatggg gtctccatca aattgagcgg gcagcacact
     3601 tttttcaagg agtgctacct gctccccatg actgtcggtt cgccatgaga cgacttcttt
     3661 ccagggccat attttgttca atgatccgat catgaagccg gttaatgcag ccatcgtaat
     3721 gtggtaatgg tgcttgaagg tccaagtcaa cactcttgaa aaagtggcca aaccgaccaa
     3781 acaccccatt gcaaaaacaa cagcaacaag cccggagccg aagtcgaagt cagtcagtaa
     3841 tttcttgaca gagccgagta tgtacgagta catgcccaac aagaccaata taaaactgcc
     3901 cgaaataccg ggaagcatca aggcagaaat ggcaatagcg ccgctgacaa acacaaacca
     3961 atactcctgt attccttcgg agggagccgc tatggtgtaa taaaatgctg caatggaggt
     4021 aattaccagc gcggcgattg ttttgagatt ccaattccct acctgcctga acaagagcct
     4081 ggcagatgcc aatatcaaca cgaagaaaaa ggaccagacc aattgtggat aatgctctaa
     4141 taaatggctg attccgaaaa cccccaccac cacgccggca gccatccctg ccataagggc
     4201 tattaagaag ttgccgtcaa tgaagcgcca tgcttccttg attcccccgg atttgaatgc
     4261 tttgaaaatg cccggcccca aaacggattt gatggcattc agcaatcgct cataaattcc
     4321 cgtgatgaaa gctatggttc cgccggatac tcccggaatc acttccgcta ctcccatagc
     4381 catgcccttg agcgctgtgg ctactgcgtt ggatcgtatt tcactcatat tattgttatc
     4441 gtttccggca ggtattatga ttcataagtc tccgccgatc tcttgtttct tagtcgttta
     4501 tttttttgat ggaggaaaat ccccactaaa cttagcagca acaccggaac gagcacattg
     4561 gcccatttga tgagttccct cgacttgctg ctgtctccca tcagatattt cgcctcgatg
     4621 ggccggatgg cggcagcttt gttgcgtagg ttcatcaagc cgctgtcatc gcccagccaa
     4681 tcaatggcgt tcaccatcaa attgatgttt tcttcagaga ctcccctgcc ctgttcgttg
     4741 ctgagcgcaa aatcggcatc gccaaaaacg atgagtttcg agaccgagcc atcagcattt
     4801 ttttgctcta tcaagccggc taaaacctga ttgccaagcg gaaaatctgc ctgcgtccac
     4861 tttttttctc ctacacttaa tgtaaccggc gacagtgcaa ggccgctttt atcagaactg
     4921 aacaccaaag gagtgaatgc tgtattcgct tgatttcctg caaattccaa cggagaaaca
     4981 tacggaaaca tcacctgttg catgcctttg acggcaggat gatcggcaaa ggtgaccgcc
     5041 agagggaggt acggaaattg cacttgtgtg ttgatctgaa tggcgcccat gtattgcggc
     5101 accgtcacac ttccacactg aacatccagc actaaagagg tgtccacctt caaccctttt
     5161 gattccagcc agtactccag cccagtttgg gatggaaatg ccatgccgct ctgcatgtct
     5221 atttccatgc gattgaaagc tatcactaat gagccgcccg attgcatata gttttccaat
     5281 tgttgaaaat gttcgggtgc aaacggggtt ttcgggccgg ccactatcgc cacttttata
     5341 ctgtctgcgc tcagggtgct gctgttcatg tccaattgct caatctcata caattgcgac
     5401 aagcccctcg ccacctgcga taattcgttc aatgaagcct ctccatgccc ttgtatgaag
     5461 gcaattttgg gcttgttttg atggctcagt tttttgatgg ctgcaactaa gttgtactcc
     5521 atggatgtgc cgggctgcac atagggaatc acctccgttt cagcgccttt tttgatgact
     5581 gcccccaaaa agtcctgttg ttgtttcact tggtccttct cccggacatt gatcatcaaa
     5641 ggttgaatgc cgtaccgggc cgcttcctgc ttgacggcat ctgttacagg cgcaacgaat
     5701 ttgtaatcta atttaccgtg cgccaccgaa gacagttcca ccaacaggtc ttgaaaatcg
     5761 cggcgcaccc ggcccacatc gggaggcaaa ccttctgaaa aataagcagt tatggttaca
     5821 ttttctttca aattgcccag tacggcttta gaggctgcgc tgagggtata ttgtttgttt
     5881 ttggtaaggt cgaggcgcag caatacgggc tgaatcagca cattcaacgc tacgatgatg
     5941 ccgatgaaca gcaataccgg caagcctttt tttatattga aagacttcat tttccagcag
     6001 ttaagtttct gattgtcagc aacaaagcga tgagggtaac ggaagcgaag tagaatacat
     6061 ctttaacatc tacaacacct ctggaaaggg aatccaaatg tttgttcata ctcagataat
     6121 aaaacccttc gccgagcgca cccggcagga atgcgccaat ggtttcgaag agaaattgga
     6181 aaaacacacc gatgaacaag gataacagca gtgccacaat ctgattgtcg gtaaaactgc
     6241 cggccaacaa accgattgca atgtaagcag cgctcatcag catcaagccg agataaccgg
     6301 ttagtgtagc gccgtgatct atttcgccca cataagccac tgaaaaatag tacggtaacg
     6361 taagtgccaa agtaatgccc accagaatcc aggaagataa aaacttaccg gctacaattt
     6421 gggcatgtga caccggcttg gtaagcagca gttcaaaggt tccgtttttt ctttcttcgg
     6481 ccagcaaccg catggtcatg gcgggaataa aaaagaaaag cgtccatttt gctacgctga
     6541 aaaaaatgcc cagatcggct tgtttgatgg caaaaatatt actggcttgg ttgagccagg
     6601 taaaccctcc ggtaaacaac aaaaaaccgc tgatcagcag gtaagcgatg agggagtcga
     6661 agttgaagcg cagctccctt cgggctatga tccaagctgg gttcaaagta cgcatcttca
     6721 cagttccttt ttgattcggg ctgcaaaatt aagaatcgtt gccacaatca caaacgaact
     6781 ctgatgccgc tcacaattga aaatgtgtga atctgatctt tgtcgggacc tatgcctttg
     6841 gagaaatagt acagcgaatg ttggaaagtt ggttgtacaa aaacgctcca gccgcttgaa
     6901 aacctccggt ctatacctgc tgaaacagag ccggttatgt aggaattatt agccaagccg
     6961 ccaccctgca accaacctcc gtctaagttc ttggattgta agcccctgct tttgggaact
     7021 cccgtcggct ccggattggg caatctcaga ttgaagctgc tgctgtggaa tgcgtcctcg
     7081 tcggtgagca aatagctgga ttccaacact acgttgactg atgcccccaa accgccataa
     7141 attctccagc cgtttttatc caaccaattg tatttcaact ggaacggcac tgaaaccata
     7201 ttgagttcaa tatccttcaa cccctccgca ctaaagccct cgctgacact gcccgtcaca
     7261 taaagtacgg gtcgtgcctt atagatttta gcggtgtaca acataccgat ttctgtttcc
     7321 caacggcctt tttcaatggc cagagcagct cccccgctgt aacccagcga gtagcgttgc
     7381 agcgcgggaa tggtaaaagt tgttctgtcc aactcagaaa acggaccttc aacacttccg
     7441 ggagtataga tgctgttgat gtcggtactc ccgaacatgc tgatggtgaa ttttttagat
     7501 ttcgattttg gtaagatttc agcgacaaca gcaggagccg ttgcgtcaaa cgtcggcgcg
     7561 ttcaggttgt actcaatggg cttgggcaag tgaacttgaa caccgggcac taagtaagaa
     7621 ctcaactccc agagatattg gtctgcaata aactggttca cagactcctc aatctgggta
     7681 ggaagaagga atgactcctg ccctgacgaa gtattttcca tgtcagaggc acgagaagct
     7741 tgttgtgcaa gtcgttccaa ttcggtctgt tgagaatctg agtcggaata tttgttttct
     7801 aaggtattgt tcactctgtc ctgagcagtg tggcgggtgt tctgaaactc attggaggca
     7861 aaaacaattc cggtattctg agtaataata tgcgcatcaa caatccagag cagcaaagac
     7921 aatgccatca attccagcac tttagaaatg gcaatctcgc gctcccggcg gtatataagg
     7981 tctaagcgat tgaccaacgt ccagttattg gaaaggccgg cgttggagac agagggtgtt
     8041 ttttgcctta ccaatcggtc gaaaaactcc atatcctgca cttcgtcctg atgttctgcg
     8101 tccaaacgct ccctgaaacg ctcccaattt tcaggctggt aggcgggagt taccttgttc
     8161 agcttgtcct tgataatgtc gtcaaatgat ctatccttcc tcatggtaac caaactttcg
     8221 ggacgtgagt atttctttca gttttgcacg agcttttact aagttcgacc gagaggtgct
     8281 ttctgtaatg gagagcaatt caccgatctc cctatgtgag tatccttcca atacgaacag
     8341 gttgaaaact gctcgataaa caggtgtaag caattgtatg gcctccagaa tttgctgttc
     8401 cgaacacttg ctgatggcat cggcttcgtc ggaactcaaa tcaaaagcgg cgtcaatatc
     8461 ttcggttctg cgccgcactg tttttctgta aaaatcaatg gcggtattca ccatgatgcg
     8521 cctgatccag gccggcagcg aggtatcggg ctgatacttg tgaatattgc ggaacacctt
     8581 gatgaaccct tcgtgcaaaa tgtccatcgc atcttcttcg ttaccggcat agcgaagaca
     8641 caacgccatc atagtcccat aaaactcttc ataaatgagt ttttgagccc aggtctcgcg
     8701 tcggacacaa gcctttataa agtcttgttc ttcatagcct atcggcaacg aa
//
LOCUS       DAORLF010000180         8706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4383_length_8706_cov_1.991099, whole genome shotgun sequence.
ACCESSION   DAORLF010000180 DAORLF010000000
VERSION     DAORLF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_8706_cov_1.991099"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1931)
                     /locus_tag="PK198_14355"
     CDS             complement(<1..>1931)
                     /locus_tag="PK198_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39968.1"
                     /translation="LNRNFFNGDFAIQDDNVDFTFNGKLDFSQKVPEFNFKADLRHLD
                     LKSLNLSDKDLIIACKVNLDGRSNKLADMEGQGRIENLQITLDQDETFSVDRIDLESR
                     LTGPDTKIVRVLSDVVEAKVEGRFEVDKLPTLFTGHLLRNHPGFARRLGLKPPKRAPA
                     PARFAFDVNVKDSKGFNHLLSPQLAALRDVRVQGGYDDASDSLVVDFYMPDFQIGDVK
                     LDGPFFSWNAKRGQAELGAGIDVLAIKGKPFLGTPLVFQGFLYYDSLNFGLTYASKGT
                     KILDNIQLNGLLAAVDSNAFALHFDQSNLTLMNRLWLINEDNNIWFSKDSVKVSNLIL
                     THDTHRIGIESAGKKGLRLALMDFNFDLIDKVWDYEPLNFSGTFNAFLRIEDVIKMQG
                     LSLTTISPGLFINNDDWGILRIDASASDLKSRINGIYSIETDNSQIWGSGFYNLQDLK
                     GRGLLRSFEESRKKYFQFTAHLSGVPLRAAEYFLPGIITGTEGTVNGEVTFNGIAGKP
                     RLEGNLVINDGKLTIDFLKTTYHFRQALVEMDDTWLFDASGAKLYDKYGHSAQITGGI
                     RHTRLKDLRIDAVLNTPRLLAIDTKKGDSKQFYGQAIGSGYARFSGPLDRIDAFIKGH
                     VNDSTRMVIPISSDREAQA"
     assembly_gap    1932..1942
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1943..>3034)
                     /locus_tag="PK198_14360"
     CDS             complement(<1943..>3034)
                     /locus_tag="PK198_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39969.1"
                     /translation="LPTALGRKNARPDTLSWTLEMQDFDLSDGAFSLHNFRKAPVKTT
                     PDNAVDFKHLDLTGIHVRFNDVLLESDTLRGVASHISSVASGGFVLEQLAVRDVMMTP
                     KSVVLNGLEIITPQSRIGDTLAFSFENYAAFEDFTSSVGMDVRFHSASVAVQDIMAFA
                     PTLERNAFFLNNRNEKIKIDGRARGTINDLSGRNLEIELAKGATLKGRLDLKDITLPG
                     QGYFGLSLQEARTSMAVLEQIIPRFNLPANFNRLGRIQFRGDINGFVGTGFTIYGDLR
                     SDLGVANLDMTLNPNGGKEKATYAGTLQLRDFDLGAWTGDPNLGKVSLDAGVQEGRSF
                     VAAQASAKLSADLRSFSYKKYEYKNARLSG"
     assembly_gap    3035..3050
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3051..3534)
                     /locus_tag="PK198_14365"
     CDS             complement(<3051..3534)
                     /locus_tag="PK198_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39970.1"
                     /translation="MSVGKLDIALFNKVVLEDFYVQDPDCDTLFYSKTLAAGINPNII
                     TLLRSGLEINEIWLKNASLNVNRPQGAPRSNLQWLLDQFASKKEKKDKKPFRISVRRI
                     NLERVQYASADDVKGSYLLARVPQGLIRISEMDLSAKVLRVKQCVLHRPYVSIESRPE
                     I"
     gene            complement(3461..3751)
                     /locus_tag="PK198_14370"
     CDS             complement(3461..3751)
                     /locus_tag="PK198_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39971.1"
                     /translation="MDTEQIPAQEQEQAAPRNPKEGRLYKLGRWTVRVIFSLFVVFLL
                     LSLLLQVPAVQNRLARYVTAELTDYLGAGVSRQVGYCAVQQSGAGRFLCAGP"
     gene            complement(3957..5015)
                     /gene="rlmN"
                     /locus_tag="PK198_14375"
     CDS             complement(3957..5015)
                     /gene="rlmN"
                     /locus_tag="PK198_14375"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRF39972.1"
                     /translation="MTEAEITLKTDIRLLSRTDLEAALTELGEPRFRSKQILEWLWNK
                     GAVSFDEMSNLPKNLRATLDAHFCIRSIVEDKVQRSADGTIKTRFRLYDGHLIESVLI
                     PVPEEQRFTVCVSSQVGCSLTCSFCATGRMKRLRNLDAGEIFDQVVLVNRQSLETFGH
                     PLTNIVYMGMGEPLLAYKPVMESIERITSPEGLGMSPRRITVSTAGIAKMIKKLADDA
                     SKVNLALSLHAADDAKRNEIMPINEQNDLESLMESLEYFYRTTQNRISYEYIAFQDFN
                     EGLDDAANLYKLCKRFPVRVNIIEYNPIDNAPFTKASAHRIDDFARYLRDRDVMVTVR
                     RSRGKDIDAACGQLANKD"
     gene            5237..6901
                     /locus_tag="PK198_14380"
     CDS             5237..6901
                     /locus_tag="PK198_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRF39973.1"
                     /translation="MSKNLTALAARKGKGLTNNLFERIAGLAEGNDGTPDNEALARIA
                     EEFLVGDANVYGTATFYDFTRPENQGKKAYLCNGSACMLAGTQEAVRAELLRHYQPHE
                     IGEMCCLGRCHENSAFHIGGQNYSAQSIQHIADIAAGTASVDADQYAVGHLGTPVLTA
                     PFPGMKACKTAIAGMLQNGPEALLQQIKSSGLRGRGGAGFPMAFKLEACRKAPGTPKF
                     IVCNADEGDPGAFSDRYLMEHRPFSVLLGMLIAGYAAGAERGVIYIRAEYPESIAIMQ
                     EAIEALTEAGLNGERLLGSDFGFQFKLIRAQGAYICGEETALLQSIEGKRPEVRVRPP
                     YPAQEGLFGKPTVVNNVESLANLYYILTQGGEAFAQIGTARSSGTKLVSLDSSFQRPG
                     LYEVDMGTPLAQVVETLGGGFRRPVKALHIGGPLGGIVPAHKIPDLSVDFESFAQQGF
                     LLGHASIIGIPEEYPMAQYLEHLFAFTAHESCGKCFPCRIGSVRGKELLHKAQHEGYR
                     IDRQLFEDLVETLAKGSLCALGGGIPLPVRNALTYFKEELDEIFAA"
     gene            7070..>8706
                     /locus_tag="PK198_14385"
     CDS             7070..>8706
                     /locus_tag="PK198_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF39974.1"
                     /translation="MTTLVPTKSTAIAAKTAYVNGKAYDIREGETILAFLKRHKGESY
                     VPTLCDAPNLEPFGACRVCSVEIARPGNGGLKTVASCHTPIEEGLMIYPDSESVQKLR
                     RNIVELVLTDHPLDCLTCEVNGNCELQDVAAQVGIRKVRYPEGKNHLDRSKDLSHPYM
                     TSDMSKCILCYRCVRACDEVQGQLVLSVAGRGFDSKIIKGMDTTFFDSDCVSCGACAQ
                     ACPTSAISDIYQSKSVMAQEKVRTVCTYCGVGCNLEVKVSGDKVLGIQAPYNAEVNAG
                     HTCLKGRYAFAFYKHPERLRYPMIRKNGQLERATWEEVYDYLAERLLDIKAKHGGDAI
                     AGISSSRCTNEENYLMQKMIRACFGTNNIDGCARVCHSPTALGMQRTFGTGAATNSIA
                     DLKLTECILIIGANPTAGHPVTGAKLKQKAMKGTPLIVLDPRRTEIAKYATHHLQLRP
                     GTNVAVLNMMLYYIIQENLMDQDFIAQRTEGFDAFCEGILSLNIDELEAVSGVPREQA
                     RAAAITYATAKLAMSFHGLGVTEHYQGTYAVMLIADLA"
BASE COUNT         1977 a         2342 c         2392 g         1968 t
ORIGIN      
        1 ggcctgtgct tcgcggtcgg aactgatggg tatgaccatg cgggtgctgt cgtttacatg
       61 gcctttgatg aaggcgtcta tgcggtcgag cgggccgctg aaacgggcat aaccgctgcc
      121 gatggcctgg ccgtaaaact gtttgctgtc gccttttttg gtgtctatgg ccaacaatct
      181 gggcgtgttg aggacggcgt ctatgcggag gtctttgagc cgggtgtgcc gaatacctcc
      241 tgtaatctgc gccgaatggc cgtatttgtc gtacaatttg gcgcccgacg cgtcgaagag
      301 ccaagtgtcg tccatttcta ccagagcctg ccggaagtgg taggtggttt tgagaaaatc
      361 aatggtcagt ttgccgtcgt tgatgactaa attgccttcc agacggggtt ttccggcaat
      421 gccgttgaag gtaacttccc catttacagt gccttccgtt ccggtaatga tgcccggcaa
      481 aaagtactct gccgcgcgca gcggcacgcc tgagagatga gccgtgaatt gaaaatactt
      541 tttgcggctc tcttcaaacg agcgcaaaag gccgcgccct ttgaggtcct gaagattgta
      601 aaatccgctg ccccatattt gggaattatc ggtttcgatg ctgtaaatgc cgttgatgcg
      661 gcttttgagg tcggacgcag aagcgtctat gcgcagtatg ccccagtcgt cattattgat
      721 gaagagaccg ggagaaatgg tagtgaggct cagcccctgc atttttatca cgtcttcgat
      781 cctgaggaac gcattgaatg tgccggaaaa attgagcggt tcataatccc acactttatc
      841 tatgagatca aaattgaaat ccatcagcgc cagacgcaag ccttttttgc cggcgctttc
      901 gatgccgatg cggtgcgtgt cgtgggtcag tatcaggttg ctcaccttca cggaatcctt
      961 tgaaaaccag atattattgt cttcattgat gagccagagc cggttcatca gagtgaggtt
     1021 tgactggtcg aagtgcaggg caaacgcatt ggagtcaacg gcagccaaga ggccgttgag
     1081 ttgtatgttg tccaatattt tggtgccttt ggaggcgtag gtcagcccga aattgagtga
     1141 atcataatac aaaaatccct gaaaaacgag gggcgttccc agaaacggtt tgcctttgat
     1201 ggccaataca tctataccgg cgcccaattc tgcctggcct cgtttggcat tccaactgaa
     1261 aaacggcccg tccaatttta catctcctat ctgaaaatca ggcatgtaga aatccacaac
     1321 caaagaatcc gatgcgtcgt cgtagccgcc ttgaacccgc acatcgcgca gggccgccag
     1381 ttgtggcgac agcaagtggt tgaaaccttt ggagtctttt acgttgacat cgaaggcaaa
     1441 acgggcgggc gcaggagcgc gtttgggcgg cttcaatccc aagcgacggg cgaaaccggg
     1501 gtgattgcgc aacagatggc ctgtaaaaag tgtcggcagc ttgtccacct caaaacgccc
     1561 ttctactttg gcttctacca cgtccgacag cacccgaacg attttggtgt cggggccggt
     1621 cagccgcgat tcgaggtcta tgcggtccac gctgaaggtc tcgtcctgat cgagggtgat
     1681 ctggagattt tcgatgcggc cctggccttc catatcggcc aatttgttgc tgcgcccgtc
     1741 cagattgact ttgcaggcaa tgatgaggtc tttgtcggag agattgagac ttttgagatc
     1801 gaggtggcgc aggtcggctt taaaattaaa ttcgggtact ttttgtgaaa aatccaactt
     1861 gccgttgaag gtaaaatcca cgttgtcgtc ttgtatggca aaatctccat tgaaaaaatt
     1921 gcgattgagc gnnnnnnnnn nncccgacag acgggcattt ttgtactcgt attttttgta
     1981 ggaaaaactg cgcagatcgg cgctcagctt ggcgctggcc tgtgccgcca caaaacttcg
     2041 gccctcctgg acgccggcat cgagactgac tttgccgagg ttgggatcgc cggtccaggc
     2101 gccgagatcg aagtcgcgca gttggagcgt gccggcatag gtggcttttt ctttgccgcc
     2161 gttggggttc agggtcatgt ccaaattggc gacgccgagg tcggagcgca ggtcgccgta
     2221 gatggtgaag ccggtaccga caaagccgtt gatgtcgccc cgaaactgta tgcgtccgag
     2281 gcgattgaaa ttggcgggca ggttgaagcg ggggatgatc tgctccaaca cggccatgct
     2341 ggtgcgtgcc tcctgcaggc tgaggccaaa gtagccttgc cccggcaggg taatgtcttt
     2401 gaggtcgagg cgacctttga gggtggcgcc tttggccagt tctatttcga gattgcgccc
     2461 ggataggtca ttgatggtac ccctggccct gccgtctatc ttaatttttt cattgcggtt
     2521 gttcagaaaa aaggcgttgc gctccagcgt gggcgcaaat gccatgatgt cctgcacggc
     2581 taccgaagcc gagtgaaaac gcacatccat gcctaccgag gaggtgaaat cttcaaaagc
     2641 agcgtagttt tcaaaagaaa atgccagcgt atcaccgatc ctcgattgcg gtgtaatgat
     2701 ttccaaccca ttgagaacga cgcttttggg cgtcatcatc acatcccgga cggccaattg
     2761 ctccagcaca aaaccgccgg aagcaacact ggaaatatgc gaggcaactc cgcgcagtgt
     2821 atcactttcg agcagcacgt cgttgaaacg gacatgtatc ccggtgaggt ccaagtgttt
     2881 gaaatccacg gcattgtcgg gcgtggtttt caccggggct ttgcggaaat tgtgcaagga
     2941 aaaagctcca tcgctgaggt cgaagtcctg catttccagc gtccaggaga gcgtgtcggg
     3001 ccgggcattt ttcctgccca aagcagtagg caaannnnnn nnnnnnnnnn ggatttcggg
     3061 ccgggattcg atggaaacat aaggccgatg caatacgcat tgtttaacgc gaagcacttt
     3121 ggccgacaaa tccatctcgc tgatgcgaat aagcccctgt ggcacgcgcg ccaacagata
     3181 gctgcctttt acgtcgtcgg cggaggcgta ttgcacccgc tccagattga tgcggcgcac
     3241 gctgatgcgg aaaggttttt tgtctttttt ctctttcttg gaagcaaatt ggtcgagcag
     3301 ccactgcaaa ttacttcggg gcgctccctg tggacgattc acgttaaggc tggcattttt
     3361 gagccatatc tcgttgattt ccaatccaga gcgcaacaat gtaatgatat tgggattgat
     3421 gccggcagct aaggttttgc tgtaaaacag ggtgtcgcaa tcagggtcct gcacataaaa
     3481 atcttccagc accactttgt tgaacagcgc aatatccaac ttgccgactg acacctgcgc
     3541 cgaggtagtc cgtcagttcg gcagtgacat agcgggccaa gcggttttgc acggcgggca
     3601 cttgcagtaa caaagacaac agcaaaaaca ccacaaacag ggagaagatg acgcgcaccg
     3661 tccatcgtcc caatttgtac agccggccct ccttcgggtt cctgggggcc gcctgttctt
     3721 gttcctgtgc aggtatttgc tctgtatcca tgtcgaaaaa agaactgccg tgagcagcaa
     3781 agcgttggcc ggcaagagcg gtttaacttc ttcttaatga ggaggttctt tcaattgaat
     3841 cgctcgccgg gtaaacaggg gcaaagataa cactttcggc ggatgttttc agaccaagat
     3901 ctaaggcgcg ccttcatctt gcgggagatt cagctcgccc atgtttcgta cctgaatcag
     3961 tctttattgg ccaattgacc gcaggcggca tcaatgtctt tgccccggct gcgacgcacg
     4021 gtcaccatca catcgcggtc gcggaggtag cgcgcaaaat cgtctatgcg gtgagccgaa
     4081 gctttggtaa acggagcgtt gtcaatgggg ttatactcga tgatgttgac gcgcacgggg
     4141 aagcgtttgc acagtttgta caagttggcg gcatcgtcca atccctcgtt aaagtcttga
     4201 aaagcaatgt attcgtagct gattctgttt tgagtggtac gataaaaata ctccagcgat
     4261 tccatcagcg attccagatc attctgctcg ttgatgggca tgatttcgtt gcgtttggcg
     4321 tcgtcggcag cgtgcaacga gagcgccaga ttgactttag aggcgtcgtc ggccaatttt
     4381 tttatcattt tggcaatacc tgccgtactg accgtgatgc gacgcggcga catgcccagg
     4441 ccctccggcg aggtgatgcg ctcaatgctc tccatcacgg gcttgtaggc cagcaggggt
     4501 tcgcccatgc ccatataaac gatgttggta agcggatgcc cgaaagtttc cagcgactgc
     4561 cggttgacca acaccacctg atcgaaaatc tcaccggcgt cgaggttgcg taagcgtttc
     4621 atgcgccccg tggcacaaaa tgaacaggtc agactgcacc ctacctgcga cgacacgcat
     4681 accgtaaagc gctgttcttc aggcactgga atgagtacag attctatcag gtgcccgtcg
     4741 tacaggcgaa accgggtttt gatcgtacca tcggcactgc gctgcacttt gtcttccaca
     4801 atgctgcgaa tacaaaaatg cgcatcgagg gtagcccgca ggtttttggg caggttgctc
     4861 atctcatcaa aggagacggc gcctttattc cagagccatt ccaatatctg tttgctccga
     4921 aagcggggtt cacccaattc tgtgagggcc gcttccagat cggttcgcga caggagtcgt
     4981 atgtctgttt tgagggtaat ttctgcttcc gtcattttat gagatcattc caggctcttg
     5041 caaaatctga atgtttgagt atttccgccc tgaacgcttc cggcggcagt tcccaaaaga
     5101 ctgagggaat aaacagccgt agtcgccgga aagttgtcgc acttctccaa cggtggggca
     5161 tggtaaacaa atacctattc aataactttt tcctacttta gcgttccgtt ttgctctgtt
     5221 gcaaaaccca agcactatgt cgaaaaatct caccgcattg gccgcccgca aaggcaaggg
     5281 cctgaccaac aacctcttcg agcgaattgc cggcctcgca gagggcaatg acggcacgcc
     5341 cgacaacgag gcattggccc gcattgcaga agaattcctc gtcggagatg ccaatgttta
     5401 cggcacagct accttctacg atttcacccg acccgaaaat cagggcaaaa aagcatactt
     5461 gtgcaacggc agcgcctgta tgctggccgg cacgcaggaa gcggtgcgcg cagagctgct
     5521 ccgccattat cagccgcacg aaatcggcga aatgtgctgt ctgggccgtt gtcacgaaaa
     5581 cagcgccttc cacatcggcg gccaaaacta ctcggcccaa tccatccaac acatagccga
     5641 catcgccgca ggaacagcct ccgtcgacgc cgaccaatat gccgtcggcc acctcggaac
     5701 gcccgttctc actgcgccct tccccggtat gaaagcctgc aaaacggcca ttgccggcat
     5761 gttacaaaac ggcccggaag cgcttttgca gcaaataaaa agctccggct tgcgaggcag
     5821 aggcggcgcc ggtttcccga tggccttcaa actcgaagcc tgccggaaag cccccggaac
     5881 ccccaaattc attgtatgta atgccgacga aggcgatccc ggcgcttttt ccgaccgcta
     5941 cctgatggaa caccgacctt tttcggtgct gctgggaatg ctcatcgcgg gctacgcagc
     6001 cggggcagag cgcggcgtca tttacatccg ggcggaatac ccggaatcca ttgccattat
     6061 gcaggaggcc atcgaggcgc tcacggaagc cggtttgaat ggcgagcgct tgctgggcag
     6121 cgatttcggt tttcagttca aactcatccg ggcgcagggc gcatacatct gcggcgaaga
     6181 aaccgcgctt ttgcaatcca tcgaaggcaa gcgccccgaa gtgcgggtgc ggcccccata
     6241 tccggcgcag gagggccttt tcggcaaacc caccgtggtg aacaatgtgg agagtttggc
     6301 caatttgtac tacatcctca cgcagggcgg cgaggctttc gcccaaatcg gcacggcgcg
     6361 ctcctccggc accaagttgg tctcgttgga cagctccttc cagcggcccg gcctgtacga
     6421 ggtggatatg ggaacgccct tagcccaagt agtggaaaca ctcggcggcg gcttccggcg
     6481 gcccgtcaaa gcgctgcaca tcggcggccc gctgggcggc attgttccgg cgcacaaaat
     6541 tcccgatctg agtgttgatt ttgaatcctt tgcacagcag ggcttcctcc tgggccacgc
     6601 ttccatcatc ggcatcccgg aggagtaccc gatggcgcag tatttggagc atttgtttgc
     6661 cttcacggcg catgagagtt gcggcaagtg cttcccctgt cgcatcggct cggtacgggg
     6721 caaggaattg ctgcacaagg cacaacacga gggctatcgc attgaccgcc aattgtttga
     6781 agatttggtg gaaaccttgg ccaaaggttc gctctgcgct ctgggaggcg gcattccgct
     6841 gccggtgagg aatgcgctga cgtactttaa ggaagaattg gacgaaattt ttgcagcata
     6901 agtcatccag tttatcgtaa gttcatctcc aagcgggaag cctccggcga gtgaacttat
     6961 gaaaacagga aggccagata aactgcgaag cagattccgc tccgagagaa gataaacagt
     7021 gaagcagatt ccgcgaatgc gatcaacttt ttgaaaaaag attttagtaa tgaccacact
     7081 cgtcccaacc aaatcaaccg ccatcgctgc caaaaccgcc tacgtcaacg gcaaagctta
     7141 tgacatccgc gaaggcgaaa ccatactggc tttcctcaag cggcacaaag gcgaaagcta
     7201 tgtgcccacc ctctgcgacg cgcccaatct ggagcctttc ggcgcctgcc gggtatgcag
     7261 cgtggaaata gcccggcctg gcaacggtgg cctcaaaacc gtcgcctcct gccacacgcc
     7321 catcgaggaa ggactgatga tctaccccga ttcggagagc gtgcaaaagc tgcgccgcaa
     7381 catcgtagaa ttggtgctga ccgatcaccc attggactgc ctgacctgcg aggtgaacgg
     7441 caattgcgag ttgcaggacg tggccgctca ggtgggcatt cgcaaagtcc gttacccgga
     7501 aggcaaaaac cacttggatc gcagcaaaga cctcagccac ccgtacatga cctccgacat
     7561 gtcgaagtgc atcctgtgct accgctgcgt gcgcgcctgc gacgaggtgc agggccaatt
     7621 ggtgctcagc gtggcgggcc gcggcttcga cagcaagatc atcaaaggta tggacactac
     7681 cttttttgat tcggattgcg tatcctgcgg ggcctgcgca caagcctgtc ctacctcggc
     7741 catcagcgat atttaccagt ccaaatcggt gatggcgcag gaaaaagtgc gcacggtgtg
     7801 tacttactgc ggtgtgggtt gcaacttgga agtgaaagtc agcggcgata aagtgctcgg
     7861 tattcaggcg ccttacaacg ccgaagtcaa cgccggccac acctgtctga aagggcgcta
     7921 tgcctttgct ttttacaaac accccgaacg cctgcgctac cccatgattc gcaaaaatgg
     7981 gcaattggag cgcgctacct gggaggaagt gtatgattat ttggccgaac gacttttgga
     8041 tatcaaagcg aaacacgggg gcgacgccat tgccggcatc agcagcagcc gttgtaccaa
     8101 tgaagagaac tacctgatgc agaagatgat acgcgcctgc ttcggcacca acaacataga
     8161 cggctgtgcc cgtgtatgcc actcccctac tgctttggga atgcaacgca ccttcggaac
     8221 cggggctgcc accaactcca tcgccgacct gaaactcacc gagtgtatcc tcatcatcgg
     8281 ggccaatcct accgcaggcc accctgtcac cggcgccaag ctgaagcaaa aagccatgaa
     8341 gggcactccg ctcatcgtcc ttgacccgcg ccgcaccgaa attgccaaat acgctaccca
     8401 tcacctgcaa ttgcggcccg gcaccaacgt agccgtgctg aacatgatgc tctactacat
     8461 cattcaggaa aacctgatgg atcaggactt tatcgcgcaa agaacggagg gcttcgacgc
     8521 gttttgcgaa ggtattctca gtctgaacat agacgagttg gaggccgttt cgggggtgcc
     8581 gcgcgagcag gcgagagctg ctgccataac gtatgctacc gcaaaattgg ccatgtcgtt
     8641 tcatggtttg ggcgtcaccg agcattatca gggcacctac gccgtgatgc tcatcgccga
     8701 cttagc
//
LOCUS       DAORLF010000181         8698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4387_length_8698_cov_2.091982, whole genome shotgun sequence.
ACCESSION   DAORLF010000181 DAORLF010000000
VERSION     DAORLF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_8698_cov_2.091982"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..>523)
                     /locus_tag="PK198_14390"
     CDS             complement(137..>523)
                     /locus_tag="PK198_14390"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRF39975.1"
                     /translation="EVKTPAPALFARLVVDEIRRLSIAGHTCVQSFDPRSLREVHRLA
                     PDMVTALLVENVRGLEQNVKELGYVPTVYSPYFKLVNAETVKNAHAQKMRIIPWTVNE
                     TADMQALIRLGVDGIITDYPNRIPGN"
     assembly_gap    524..622
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<623..1055)
                     /locus_tag="PK198_14395"
     CDS             complement(<623..1055)
                     /locus_tag="PK198_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRF39976.1"
                     /translation="MKNHLLHLIWGAALFLLAACTPRPANMPAMNHPTAFDWQGHRGA
                     RGLAPENTVPSFLKALEFPLVSTLELDVVISKDRQVIVSHEPWMSHEICSHAGGKAVQ
                     KSEEETLIILQLTAAEIAAFDCGSRGHTRFPEQEAMQAAKPT"
     gene            complement(1088..1627)
                     /locus_tag="PK198_14400"
     CDS             complement(1088..1627)
                     /locus_tag="PK198_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39977.1"
                     /translation="MKKFILLALLLSAWNTQIFSAVAAVMPALEAVVETEAALAPALS
                     LGELTEMTPREWEAHSGDKLSFGERLAFKMVQKKIKKQAAKDKLDLSAPAGSETMSTL
                     SLIFSVVGLALLFVPLIGILGFILGISGFVLGLVAFKKEGTNVKNILGVVFGGLMILF
                     LLLGIVFVAIAIAAAASIR"
     gene            1941..>2396
                     /locus_tag="PK198_14405"
     CDS             1941..>2396
                     /locus_tag="PK198_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRF39978.1"
                     /translation="MRWSLTLFALFISALAWGQMKNPVKWSTSYKQVSATEFDLVFTA
                     TIEDGWTVYSQFLDSDDGPVPTSFNFDKGDHYQLKGKCSESGNIKTVYDKVFEMNLTK
                     ITKKGVFTQRVTATDLSKPITGYLEFMTCDDEQCLPPKSVDFEFLLKDSN"
     assembly_gap    2397..2464
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2465..4553
                     /locus_tag="PK198_14410"
     CDS             <2465..4553
                     /locus_tag="PK198_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRF39979.1"
                     /translation="TPAPGQQKQEVPAAPTITDIAPAPAAPKMYDPAKWQFDYKKISD
                     TEYDLILTATLDKGWNIYSQFTDPNNGPLPTTFTFTPGEHFSLEGKMQETGKKKQAPD
                     PLFANALVVKFTESPVVFTQRVKVTDATQPIIGFAEYMVCDDGQCTTGAKDFRFNLSE
                     AAASAMVGPKLEGNVIDQVVPALQAGYKEPLSDCGQEKTGDRSSLLWIFLGGFLGGLL
                     ALLTPCVFPLIPLTVTFFTKRSIDRKTGLRNALLYGLSIIVIYVAIGLFITAFFGETA
                     LQALSTNWIANTLFFVIFVAFALSFFGLYEITLPSSWGTKSDTMAEKGGLIGIFFMAA
                     TLAIVSFSCTGPIIGTALVQSATDKLGPFVVMLGFSTALALPFALFAAFPGWLNSLPK
                     SGGWMNLVKVQLGFLELALALKFLSVADMTMHWGILPYEVFMGLWILIAALMALHFFG
                     ILRFSHDSREKKLPAYRLVLGSLMALAVVYLALGFRFDERLQSYKSPGALSGLAPPAR
                     YNLFLKDLNDDLDPDIKSRYPSYAKSANNLNCFKDYYEGLAYAREVNKPILLDFTGHG
                     CVNCRKVEEHIWIDERVWKKINDEYVLISLYTDDRERLEEMLLSKHRPGEKLRNVGNK
                     WADFQIVNFVQNTQPLYVLVSPNEEVLAPARGFEEGVKKYADFLDCGLFTFKNLSQQK
                     QPATGSPEVGSAE"
     gene            complement(4661..5914)
                     /locus_tag="PK198_14415"
     CDS             complement(4661..5914)
                     /locus_tag="PK198_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRF39980.1"
                     /translation="MKVHIITVGDEILIGQIIDSNSAYMGRLLNLNGARVERIISIGD
                     RHEEIVHTLAQSVAQADVVLMTGGLGPTKDDITKKALADYFGVEMVFSEETYRRILKI
                     FEQLGRPATEAHRHQCYMPANAALLYNKMGTAPGMWMQAESGAVVVSMPGVPYEMEYL
                     MEHEVAPRLRAHFPGKPIGHRTLRTAGEGESRIAERLDAFEETLPEGMKLAYLPNPGQ
                     VRLRLSFSADTQQELDAALDTKAAELHALLPDIVYGFGEDELETVLGTLLMERKLHIG
                     TAESCTGGYLAHRITAVPGASRYFNGAIVSYSNEVKMKSLGVSETTLREHGAVSEATV
                     REMVKGALQALPIDVAVAISGIAGPDGGTPDKPVGTIWMAVGNADRIETHLIKGGKHR
                     LRNIQYAGTLALEMTRRFLVGLPGV"
     gene            6075..>6810
                     /locus_tag="PK198_14420"
     CDS             6075..>6810
                     /locus_tag="PK198_14420"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRF39981.1"
                     /translation="MHLSEQGIYYGDYLQLDKILNAQDLESEKAGKTAHDEMLFIVTH
                     QAYELWFKQVIYEIDSILCLFEQPVIHDNSPDFYTIFHRLSRVKTILQLLVKQIDIME
                     TMTPLDFLDFRNLLRPASGFQSVQFKVVEAALGLKQPDRHGELYYISQLRPEHAAAIQ
                     KIEERRSLLELTNEWLERMPFFKDAGLWERYAPAFPVQEGLHPFWSDYFQLYGLSLLD
                     NERGNLTRFERFFINDEETPFGKLSFE"
     assembly_gap    6811..6833
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6834..7143
                     /locus_tag="PK198_14425"
     CDS             <6834..7143
                     /locus_tag="PK198_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRF39982.1"
                     /translation="ALFIMLYRDFPLFHLHYQYLSLLLDMDEMMSTWRFRHINMVNRI
                     IGLRTGTGGSTGKDYLKAALDRHYIFNEIAALTTFLIERRSLPVLPAELQQRLGFVVK
                     "
     gene            complement(7147..7914)
                     /locus_tag="PK198_14430"
     CDS             complement(7147..7914)
                     /locus_tag="PK198_14430"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRF39983.1"
                     /translation="MKNTLILLIAVIAALVLTACSGPQKDAATETTKEEVPELYAGYA
                     AVPPIIDGMPGDSCWRNLPWYPIDQIWIGDSIDHQDFSGKYKVAWSEDYLYILAEIRD
                     DTLIDIHPDGLAFYWDDDCLEIFVDEDASGGNHQYSHNAFAYHIALDGKVADIGPDSL
                     PHYYDHHIQQRRSTIGNRSRWEVAIALYPDTYRDGASDNHPVKLHQGKTIGFALAYCD
                     NDRSPERENFFGSVFVPGEDKNRGWIDAGIFAKLPLR"
     gene            complement(8005..8370)
                     /locus_tag="PK198_14435"
     CDS             complement(8005..8370)
                     /locus_tag="PK198_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39984.1"
                     /translation="MEDQLKHIKQQAESYRMQPSQQAWERLERRLGEQPKSAVVRRLS
                     LRHWAAAASVLGMAAIAALLLLRPDNSAQPESAAVMEMQQAPELEMLDMTNTDEVALM
                     AVNYTRFLERNHPEMMRDF"
     gene            8369..>8698
                     /locus_tag="PK198_14440"
     CDS             8369..>8698
                     /locus_tag="PK198_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39985.1"
                     /translation="MIPDIFSTAVGHEFCLSKKVSRWNGCWPLAAGLKLLAASRKPCG
                     EHHSRFFSFSRKLVRRGGFAPFAKIRVKTRVSGFLVQGSTRQDKLRSRLPLSPKPPLL
                     QNILHPFF"
BASE COUNT         1873 a         2369 c         2249 g         2017 t
ORIGIN      
        1 cctcatcatt accaacaacg agggcttcga caatggcctg gctgtactca ccggcttcat
       61 ccggcactac atgcaaaacc ccgcgcactg gcacgacccg gagttttgag tacataaggg
      121 ggccctgact gaagtttcag tttcccggaa tccgattcgg ataatccgtg atgatgccgt
      181 ccacgccgag gcggatgagc gcctgcatat cggcggtttc gttcaccgtc cagggaatga
      241 tgcgcatttt ttgagcgtgc gcatttttga ccgtttccgc attgacgagt ttgaaatacg
      301 ggctgtaaac cgtcggcaca taccccaatt ctttgacgtt ttgctccaga ccgcgtacat
      361 tttctaccaa caaagccgta accatatcgg gcgctaaacg gtgtacttcg cgcaggctgc
      421 gggggtcgaa cgattgcacg caagtgtggc cggcaatgct caatcggcgg atttcatcca
      481 ccacaagacg ggcaaaaagg gccggagcgg gcgttttgac ttcnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nngcgtcggt ttggcggcct gcatggcctc ctgttccggg
      661 aaacgggtat ggcccctgct gccacagtcg aaggcggcta tttcggcagc cgtcagttgc
      721 aggataatga gcgtttcctc ttcgcttttt tgcacggctt ttccgccggc atggctgcaa
      781 atttcatgcg acatccacgg ctcatgcgat acaatgactt gacggtcttt ggaaatgacc
      841 acgtctaatt cgagggtgga aacgagcgga aactccagcg ctttcagaaa agaaggcacg
      901 gtgttttcgg gcgccaggcc gcgcgcgccg cggtggccct gccagtcgaa ggcggtggga
      961 tggttcatag caggcatatt ggcaggccgg ggcgtacagg ctgcgagcag gaaaagggct
     1021 gcgccccaga tgaggtgtaa aagatggttt ttcattcgga atcagtatgg atgatgatgt
     1081 ttttttctca acgaatagaa gccgcagctg ctattgctat cgccacaaaa acaataccga
     1141 gcagcaggaa cagaatcatc agacctccaa acacgacgcc cagaatgttt ttcacgttgg
     1201 ttccttcttt tttgaacgct accaagccca gcacgaatcc tgagatgccg agtataaagc
     1261 ccagaatgcc gataagaggc acaaacagca gtgccaggcc cactactgaa aagataagcg
     1321 acagggtact catcgtttca ctcccggccg gggcggagag gtcaagtttg tctttggcgg
     1381 cttgtttttt gatctttttt tgcaccattt tgaaggcaag ccgctcgccg aaagacaact
     1441 tatcgccgct gtgggcctcc cattcgcggg gcgtcatttc tgtcagctct cccaaagaga
     1501 gcgcaggcgc aagggcggct tccgtttcca caacggcttc cagcgcgggc atcacggcag
     1561 ctactgcact gaatatctgg gtgttccatg ctgagaggag aagggctaaa aggataaatt
     1621 ttttcatggc agaaaatttt tgggtgaaag aatttgaact tataagaccg gagccaaagg
     1681 cttggacgtt ggcttcgggc gaaaacacaa cgcaagatat tgtctttttc agccgggggt
     1741 ggttacggca atgttaaatg tattacacag ccatccgccc gcttttgtaa cccgcatcac
     1801 agcacaagcc cctgaacgcc tgctaatttt gcggcgccgg aacggaaggc gtcaactgtt
     1861 aatgtttact tattgtttac ccttcgttcc cccgaaaggg gttatttttg cggctctaac
     1921 tgaaaaacat cattcaaaat atgagatggt ctctcacgct tttcgcgctg tttatttccg
     1981 ccctcgcctg ggggcaaatg aagaatcctg taaaatggtc cacctcctac aagcaggtct
     2041 ctgccaccga gttcgacctc gtttttaccg ccacgattga agacggctgg acggtctatt
     2101 cgcagtttct cgacagcgac gacggcccgg tacccacttc cttcaatttc gataaaggcg
     2161 atcattatca gctcaaaggc aaatgctcag agtcgggtaa catcaaaacg gtgtacgaca
     2221 aggtttttga aatgaacctc accaagatta ccaaaaaagg cgttttcacc cagcgcgtta
     2281 cagctaccga tttgagcaag cccatcaccg gctacctcga atttatgacc tgcgacgacg
     2341 agcagtgcct gccgcccaaa tcggtggatt ttgagttcct gctcaaagat tccaacnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnaacgcc cgcccccggt cagcaaaagc aggaagtacc cgccgcccct accataacgg
     2521 acatcgcccc ggcgcccgct gcgcccaaga tgtacgaccc tgcgaaatgg cagtttgact
     2581 acaaaaaaat cagcgatacg gagtacgatc tcatcctgac tgctacattg gacaaaggct
     2641 ggaacatcta ctctcaattt accgatccca acaacggccc gctgcccacc accttcacgt
     2701 ttacgcccgg cgagcatttc tcgttggaag gcaaaatgca ggaaaccggc aaaaaaaagc
     2761 aggcgcccga tcctttgttt gccaatgcct tggtggtcaa attcacggaa agccccgttg
     2821 tgttcaccca gcgcgtgaaa gtcaccgacg ccacgcagcc catcatcggc tttgcagaat
     2881 atatggtgtg cgacgacggg caatgcacca ccggcgccaa agacttccgc ttcaacctca
     2941 gcgaagccgc cgcctctgcg atggtaggcc ccaagttgga aggcaatgtg atagatcagg
     3001 tcgttccggc attgcaggca ggctacaaag aaccgctgag cgattgcggc caggaaaaaa
     3061 ccggcgaccg cagttccctc ctttggatat tcctgggagg tttcctcggc ggcctgctgg
     3121 cgctgctcac gccctgcgtg tttccactca ttccgctcac cgttacattt ttcaccaagc
     3181 gcagcataga ccgtaaaacg ggcctgcgca acgcattgct gtatggactt tccatcatcg
     3241 ttatttatgt ggccatcggc ttgtttatca cggcgttctt tggcgaaacg gcacttcagg
     3301 cattgtccac caactggata gccaataccc tgttcttcgt catttttgta gcttttgcgc
     3361 tgtctttctt tggtttgtac gaaataacgc tgcccagcag ctggggtacc aagagcgaca
     3421 ccatggccga aaaaggcggt ctcatcggta tcttttttat ggcggctacc ctggccattg
     3481 tttcgttttc ttgcaccggc cctatcatcg gcacggcgct ggtgcaatcg gctaccgaca
     3541 aactggggcc gtttgtggtc atgctcggtt tttctaccgc attggccctg cccttcgccc
     3601 tgtttgcggc tttccccggc tggctcaaca gccttcccaa atcgggcggc tggatgaatt
     3661 tggtgaaagt acaactcggt tttttagaat tggctctggc cctcaaattc ctctctgtgg
     3721 ccgatatgac catgcactgg ggtattctgc cttatgaggt gttcatgggg ctgtggatac
     3781 tcattgccgc actgatggcg ctgcatttct ttggtatcct gcggttctcg cacgacagcc
     3841 gcgagaagaa gttgcccgcc tatcgcttgg tgttgggcag cctcatggca ctggccgtcg
     3901 tatatctggc cctcggtttc aggtttgacg aaagattgca gtcgtacaaa tcgccgggcg
     3961 cactcagcgg tttggccccg ccggctcgct acaacctgtt tctcaaagac ttaaacgacg
     4021 atctcgaccc ggacatcaag tcccgttatc cgtcttacgc caaatcagcc aacaacctca
     4081 actgcttcaa agactactac gaaggtctgg cctacgcccg cgaagtgaac aaacccatcc
     4141 tgctcgactt caccggtcat ggctgcgtga actgccgcaa ggtggaggaa catatttgga
     4201 ttgatgagcg cgtttggaaa aaaatcaacg acgagtatgt gctcatctcg ctctataccg
     4261 acgaccggga gcggctcgaa gaaatgttgc tttccaagca ccgcccaggt gaaaaactgc
     4321 gcaacgtggg caacaagtgg gccgatttcc agatcgtcaa ctttgtacaa aatacgcagc
     4381 ctttgtatgt cctcgtcagc cccaatgagg aggtactcgc ccctgcgcgc ggcttcgagg
     4441 aaggggtgaa aaaatatgcc gacttcttag actgcggtct gtttactttc aaaaacctgt
     4501 cgcaacaaaa acagccggct accggatcgc cggaagtagg gtcggcggaa tgatcccgcc
     4561 aagtcagggc gtacccgaag ttacggcctg accccatgcc ggaggcagtg aaggggtgac
     4621 cacacgaggc taagtcttgg cggagtcgcc ccttcactca ttatacccca ggcaagccca
     4681 ccaaaaagcg gcgcgtcatt tccagcgcca gcgtacctgc gtactgtata ttcctgagcc
     4741 ggtgtttccc tcctttgatg agatgcgttt ctatccgatc cgcattgcct accgccatcc
     4801 agatggtacc caccggctta tcgggggtgc cgccgtcggg gccggcaatg cccgaaatgg
     4861 ccaccgccac atctatcggc agcgcctgca acgctccttt caccatctcc cgcacggtgg
     4921 cttcactgac ggcgccgtgt tcccgaagtg tcgtctcact cacgcctaaa ctcttcattt
     4981 tcacctcatt gctgtacgat acaatagcgc cgttgaaata ccgcgacgcg ccgggcaccg
     5041 ccgtgatgcg atgtgccaga tagcctccgg tgcagctttc cgccgtgccg atgtgtagct
     5101 tgcgttccat cagcaacgtc cccagcaccg tttccaattc gtcctcgccg aagccgtaaa
     5161 cgatgtcggg aagcaaggcg tgcaactcgg cggctttggt atccaatgcg gcatccaatt
     5221 cctgctgcgt atcggcgctg aagctcaggc gcaggcgtac ctgtccggga ttgggcaggt
     5281 aggccaattt catgccctcc ggcagggttt cttcaaaagc atccaagcgc tcggcaatgc
     5341 gcgattcgcc ctcgccggca gtgcgcaggg tgcggtggcc gatgggtttg ccggggaagt
     5401 gtgcgcgcaa tcggggcgcc acttcatgct ccatgaggta ctccatttcg taaggtacgc
     5461 cgggcatgga gacgacgacc gcgccacttt ccgcctgcat ccacataccc ggagcggtgc
     5521 ccattttgtt gtacagcaga gcagcgttgg cgggcatgta gcattggtgg cggtgcgcct
     5581 cggtagccgg gcggcccaat tgttcaaaga ttttcaatat gcggcggtag gtctcctcgc
     5641 tgaagaccat ctctacgccg aagtagtcgg ctaaggcttt tttggtaata tcgtctttgg
     5701 taggaccgag gccgccggtc atcagcacca catcggcctg agcaacggac tgcgccagag
     5761 tatggacgat ttcctcatgt cggtcgccga tggaaatgat gcgctctaca cgagcgccgt
     5821 tgagattgag caggcggccc atgtaggccg agttggaatc aatgatctgg ccgatgagga
     5881 tttcatctcc tacggtaatg atgtgtactt tcataaagtc gcggattgtg gaatatggcc
     5941 cgaaaggtac gccaaaagcg aagaatatta tttttgcatt ccggtctgag atgcggcttt
     6001 ccaccatcag gagttgcaag catttgaaag tcaggccata cattttccaa acgtattcta
     6061 accaaatcaa aaccatgcac ctctctgaac aaggcattta ttacggcgac tatctgcaat
     6121 tggataaaat cctgaacgcg caggacttag aaagcgaaaa ggccggcaaa accgcccacg
     6181 acgaaatgct tttcatcgtc acgcatcagg cttacgaact gtggttcaag caggtgatct
     6241 acgaaatcga ctccattctc tgcctgttcg agcagccggt catccacgac aactcgcccg
     6301 atttttatac cattttccat cgcctcagcc gggtgaagac cattttgcag ttgttggtca
     6361 aacagattga catcatggaa accatgaccc cgctcgattt tctcgacttc cgcaacctcc
     6421 tgcggccggc ctccgggttc cagagcgtgc agttcaaagt ggtggaagcg gcgctgggcc
     6481 tcaaacagcc cgaccgccat ggagagctat actacatctc gcaattgcgc cccgaacacg
     6541 cggcggccat ccaaaaaatc gaggagcgcc gctcgctgct ggaactgacc aacgagtggc
     6601 tcgaacggat gccttttttc aaagatgccg gtttgtggga gcgttatgcg ccggcttttc
     6661 ctgtgcagga ggggttgcat ccgttttggt cggattattt ccaactgtac ggcctcagtc
     6721 ttttggacaa tgagcgcggc aatctcaccc ggtttgagcg cttttttatc aacgacgaag
     6781 agacgccttt cggcaaactc agtttcgagg nnnnnnnnnn nnnnnnnnnn nnntgcgctg
     6841 ttcatcatgc tataccgcga ttttccgctg tttcacctgc attaccaata cctcagcctg
     6901 ctgttggaca tggacgaaat gatgtccacc tggcgtttca ggcacatcaa catggtgaac
     6961 cgcatcatcg gcctgcgcac aggaacgggc ggcagcaccg gcaaagacta cctgaaagcg
     7021 gccttagacc ggcactatat ttttaatgaa attgctgcgt tgacaacctt cctcattgaa
     7081 cggaggagcc tgcccgtttt gccggcggag ctacagcagc ggctggggtt tgtggtgaag
     7141 taggtatcac ctcagcggca gctttgcaaa aatccccgca tctatccagc cccggttttt
     7201 gtcttcgccg ggcacaaaaa ccgagccgaa gaagttttcg cgttcggggc tgcggtcgtt
     7261 gtcgcaataa gccagggcaa acccgatggt tttgccttgg tggagtttta cggggtggtt
     7321 gtcggaagcg ccgtcgcggt aggtatccgg atagagggcg atggctactt cccaacggct
     7381 tctgttgccg atggtgctgc ggcgctgctg gatatggtga tcgtagtagt gcggcaggct
     7441 gtcggggccg atgtctgcca ccttaccgtc taaggcgatg tgataagcaa aagcattgtg
     7501 gctgtactgg tggttgccgc cggaagcgtc ttcgtccaca aaaatttcga ggcagtcgtc
     7561 gtcccagtaa aatgccaggc cgtcgggatg gatgtctatc agtgtatcgt ctcggatttc
     7621 agccaaaatg tagaggtaat cttccgacca tgcgactttg tattttccgc taaaatcctg
     7681 atggtcaata gaatctccga tccaaatttg atctatcggg taccagggca ggttgcgcca
     7741 gcaggaatcg ccgggcatac cgtctatgat ggggggcacg gcggcatagc cggcgtaaag
     7801 ttctggaact tcctctttgg tcgtttcggt agcggcgtcc ttttgggggc cggaacaggc
     7861 cgtgagcact aaggcagcta taacagcaat gagcagaatg agcgtgtttt tcatggcaga
     7921 gattcaaaaa aaagtgatgc aaattccaaa gtccgccaaa tgtacgttct tctttttgat
     7981 ctgcctgcca tcaggcattt gaacctaaaa atccctcatc atctccgggt ggttgcgctc
     8041 caaaaagcga gtatagttta ctgccatgag cgctacctcg tcggtattgg tcatgtccaa
     8101 catttctaat tcgggcgcct gttgcatttc catcacggcg gcagactccg gttgcgcact
     8161 gttgtcgggc cgcaacaaca gcaacgctgc tatggctgcc atgcccagca cagaggcggc
     8221 tgccgcccaa tgccggagcg acaggcgacg taccaccgcg cttttaggtt gctcgccgag
     8281 acgacgttct aagcgctccc atgcttgctg cgagggctgc atccggtatg attcggcctg
     8341 ctgtttgata tgtttcaatt gatcttccat gattcctgat atattttcca cggctgtcgg
     8401 ccatgaattt tgtttgtcaa aaaaggtttc gcgttggaac ggctgctggc cgctggctgc
     8461 cggcctcaag ctgctggctg caagccgcaa gccgtgcggg gagcatcatt cgcgtttttt
     8521 ctcgttttcg cgaaagcttg tccgccgggg cggattcgcg cccttcgcca agattcgcgt
     8581 taaaactcgc gtttccgggt ttctggttca aggttccact cgacaagata aactgcgcag
     8641 cagattaccc ctctcaccca aacccccgct tctgcaaaat atcctgcatc cgttcttt
//
LOCUS       DAORLF010000182         8646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4422_length_8646_cov_1.805378, whole genome shotgun sequence.
ACCESSION   DAORLF010000182 DAORLF010000000
VERSION     DAORLF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_8646_cov_1.805378"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK198_14445"
     CDS             complement(<1..267)
                     /locus_tag="PK198_14445"
                     /inference="COORDINATES: protein motif:HMM:NF037126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39986.1"
                     /translation="MKFSQRIGKTAVREALQIESMDQALENRLWNVIYTYFFSLVNVS
                     MGSTEATVQGVQCQIIWENFFVKRIDELPYRSDNMTLLMVYDYTK"
     gene            complement(385..>1639)
                     /locus_tag="PK198_14450"
     CDS             complement(385..>1639)
                     /locus_tag="PK198_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRF39987.1"
                     /translation="IRANVDTLTLTATPIPRTLQFSLMAARDLSVIRTPPPNRQPIHT
                     EVRVFSEALIKEAIYYEVNRGGQVFFVHNRVKSLGDMTAMLQRLCPDVEFRMAHGQLE
                     LDRLEKTLVDFIDKQFDVLVSTNIIETGLDIPNANTIIITDAHHFGMSDLHQLRGRVG
                     RSNRKAFCYLFCPPMSTLTSDARKRLRTIEEFSDLGSGFQIAMRDLDIRGAGNLLGAE
                     QSGFIADIGFETFQRILDEAMRELKETEFKDLFEDELAKDPRYVGDIIIDTDVEMLIP
                     SEYVSNITERLALYTELDAIETEEALEQYADKLRDRFGRLPVQVRELFEGLRLRWVCR
                     KLGFERIILKNNKLRAYFVENPQSSFYETATFHAILAHVAKQGPAQGLSFKQSNKSFI
                     LVKDGVKSLKAAKGGLEGLLAVVSG"
     assembly_gap    1640..1672
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1673..3758)
                     /locus_tag="PK198_14455"
     CDS             complement(<1673..3758)
                     /locus_tag="PK198_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRF39988.1"
                     /translation="MSNLHQLLDLFRKDRRNRNLLEALQQHSPARVLLNGITGAQQAM
                     AVAAAFLEQPRTHVLIASDKEQAAYLQNDLAGLLEQKPVLFFPDSFKRPAHFEEINST
                     QTLERADTVNRIATTDKAGLIVVTYPEALLERVVAPAALDQSRIDINKGERLDVDTII
                     DVLVEHGFAREDFVFEPGQFSIRGGIVDIFSFGNDQPYRIELFDDEVESIRTFNPLTQ
                     LSVQNISRVSIVPNIHSKFSPTQKVPFFEVLPPDRVLWIRDMEMMKAQLLHCAERVEQ
                     KTPEISKLDESDLAVLFREGHFLTPDTVAEAVLKCSLVLTGTEERQPLTVNRIVAYHS
                     NPQPAFNKQFNLLIDNLQKNARNGIVNYLFTDNPRQVERFYSIFEDLKADVKFHPVIC
                     AIHEGFVDNDLKVACYTDHQIFERFHRYKLKQGYTKEMALNVRMLNELQPGDFVTHID
                     HGIGKYSGLEKININGHIQEAVRLIYKNNDILYVSINSLHKIAKYAGKDSTEPQVNKL
                     GSEAWKNLKAKTKKKVKDIAKELIQLYAKRKASPGFAFAPDGYLQTELEASFIYEDTP
                     DQLKATNEVKDDMLKDYPMDRLICGDVGFGKTEVAVRAAFKAILDKKQVAILCPTTIL
                     ALQHHKTFSDRLKEFGVEADYLNRFRSTAERKDVVARLEKGDIDIARELTPTDIEGLA
                     GNADLKVTDDPGG"
     gene            4031..>4347
                     /locus_tag="PK198_14460"
     CDS             4031..>4347
                     /locus_tag="PK198_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39989.1"
                     /translation="MIGTKNYFDTIIANQQQLMATFTEYTNKAVEAMTPNKEVAEKGA
                     ALMNEMMTRPYELAGEMAKRETLEKFQADFWGTYSEHLNKSTDLAADLYRKSFEYMKE
                     MWG"
     assembly_gap    4348..4386
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4678..>4921
                     /locus_tag="PK198_14465"
     CDS             4678..>4921
                     /locus_tag="PK198_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39990.1"
                     /translation="MKNAWDSVLDNQKKVMDFWGNMAQQMTPNTKKAEAPADMFTQWM
                     EQQKSVWEQWMKGMKPEDAMTAMPEQLRQWAEMQQKF"
     assembly_gap    4922..4938
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4939..>5291
                     /locus_tag="PK198_14470"
     CDS             <4939..>5291
                     /locus_tag="PK198_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39991.1"
                     /translation="VEMYRENAGKMGLGMPQMDMSAFMPNRMMEENVKNWKNWTQSSE
                     KWMRDAVMAKMPFNMQPHYSNFIEFFDDVQRYWEPFSRMIQNGIFDKAMVEKYFDQNA
                     YLKVVNQLMGFKPVG"
     assembly_gap    5292..5307
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5308..>6004
                     /locus_tag="PK198_14475"
     CDS             <5308..>6004
                     /locus_tag="PK198_14475"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HRF39992.1"
                     /translation="GNVTELIDSVNHYFEKMMGQMGDEASKWNTVSDTWRSKMSEVFS
                     GSHAPLLQFGNDINNRLRDQLTPFFNIAAQGRETEIAKLMRDVQFSYISFILKTSELQ
                     SKVYDAGQYVLPDLLRAYYEDYQKTKEMTDFQAFLHKYINELENSILTVLNADEYSKL
                     QSEIAALGASIKLKSDKATELVLADMPFLTRSEGDDFAQEAAALRKKVRSLEQRLAAL
                     EQHLGAEAEVVAAE"
     assembly_gap    6005..6039
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6137..>6812
                     /locus_tag="PK198_14480"
     CDS             6137..>6812
                     /locus_tag="PK198_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF39993.1"
                     /translation="MSHAKALSEQIIERVDTAAEALKNINDIQDVDIATAQREVVWND
                     GKLKLYHFHREGEAKAHTPMLVSYALVNRWEMMDLQPNRSFIRKLISEGIDVYLIDWG
                     YPTKIDRYKTLEDYVLGHINDCVDVVRQRHNLQKINLLGVCQGGTFSLMYSALFPEKI
                     KNLITLVTPVDFDNEEGLLFKWAKDMDVDAVVDGFGGIIPGDFLNTGFDLLKPMNKTR
                     KYMALPD"
     assembly_gap    6813..6981
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6982..7206
                     /locus_tag="PK198_14485"
     CDS             <6982..7206
                     /locus_tag="PK198_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HRF39994.1"
                     /translation="RTVDLSKINMPILTIYAKDDHIVPPATTKPINDLVSSKDKELME
                     FPGGHIGVFVGARSQDLLTPAIAKWLVERD"
     assembly_gap    7231..7241
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7536..>8646)
                     /locus_tag="PK198_14490"
     CDS             complement(7536..>8646)
                     /locus_tag="PK198_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF39995.1"
                     /translation="PTNPATWTARTWQLNSSLPRSRPGTGISAANAYVGNCSSDLENG
                     IQRLQSPVISIPPTAATTLRLSFEHYVSLESTWDGGNLKYSKNGGAWTLLPASAFVFN
                     AYNSSLQTVGAGNDNPLAGQTAFTAANEGSVAGSWGQSQINLAAIGIAPGDNVQLRWE
                     LGEDGCNGWDGWYLDDVVLCQCQSALPVSLLSFSAEAEKSSVQLRWATAQERNNAGFE
                     VQRRDGWEMEFKTIAWAPGKLDADLRSDYAHEDADVRPGLSYYYRLRQVDADGTGTFS
                     QVVAARIKPEETVDLHLFPNPTHDLLFLQPEGSWGAQLNVSLTDLLGRVVHDARLEHQ
                     TADAMSLSLAHLPPGMYLLRAGDGLRSLVRKVWVR"
BASE COUNT         1928 a         2186 c         2333 g         1879 t
ORIGIN      
        1 cttcgtataa tcgtaaacca taaggagagt catattatca gagcgatatg gtaattcgtc
       61 aatacgttta acaaaaaaat tctcccagat aatctggcat tgtacacctt gaactgtggc
      121 ttcagtactc cccatgctaa cattcacgag actaaaaaaa taggtataaa ttacattcca
      181 cagcctattt tccagcgcct gatccattga ttcaatttgg agcgcctccc ttacagcagt
      241 tttaccgatt ctttgagaaa atttcatgcg acttaacgat tagctcctaa aggtagtcat
      301 atcttcaaaa ttccactcac ccctcactcc ctcacccctc actccctcac tcactcaccc
      361 ctcactcact cacccctcac tccctcaccc actcaccact gccaacagcc cctccaaacc
      421 ccccttcgcc gccttcaagc tcttcacccc gtctttgacc aaaatgaagc tcttgttcga
      481 ttgtttgaag ctcaggccct gcgccggacc ctgcttggcc acatgcgcga ggatggcgtg
      541 aaaagtggcg gtttcgtaaa aagaggactg cggattttct acaaaatacg ctcgcaactt
      601 attgtttttc agaatgatgc gctcgaagcc caacttgcgg cacacccagc gcaggcgcag
      661 cccctcgaac aactcgcgca cctgcacggg cagcctgccg aagcggtcgc ggagcttgtc
      721 ggcgtattgt tcgagcgctt cttcggtttc gatggcgtcg agttcggtgt agagggcgag
      781 gcgctcggtg atgttgctga cgtactcgct ggggatgagc atttccacgt cggtgtcaat
      841 gatgatgtcg ccgacgtagc gcgggtcttt ggccaattcg tcttcaaaga ggtctttgaa
      901 ctccgtttcc ttgagttcgc gcatggcttc gtcgaggatg cgctggaagg tctcgaagcc
      961 gatgtcggcg atgaagccgc tttgctctgc gccgaggagg ttgcctgcgc cgcgaatgtc
     1021 caagtcgcgc atggcaatct ggaagccgct gccgagatcg gaaaactcct cgatggtgcg
     1081 gaggcgtttg cgggcgtcgg aggtgagggt ggacatgggc gggcagaaga ggtagcagaa
     1141 tgccttgcgg ttggagcgcc ctaccctacc ccgcagttgg tgcaggtcgc tcatgccgaa
     1201 gtggtgggcg tcggtgatga tgatggtgtt ggcgttggga atgtctaagc cggtttcgat
     1261 gatgttggtg ctgacgagca cgtcaaattg cttgtcaatg aagtccacca gcgttttttc
     1321 gaggcggtcg agttcgagtt ggccgtgcgc catgcggaac tctacgtcgg ggcagaggcg
     1381 ctgaagcatg gccgtcatat cgcccagcga tttgacgcgg ttgtgtacaa aaaagacctg
     1441 tccgccgcga ttgacctcgt aataaatggc ctctttgatg agggcctcgc tgaacacccg
     1501 cacctcggtg tggatgggct ggcggttggg cggcggcgtg cggatgacgg agaggtcgcg
     1561 ggcggccatg agcgaaaact ggagggtgcg cgggatgggc gtggcggtga gggtgagcgt
     1621 gtccacgttg gcgcggatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggccgccc
     1681 ggatcgtcgg tgaccttcag gtcggcattg cccgccagcc cctcgatgtc ggtgggcgtc
     1741 agttcgcgcg cgatgtcgat gtcgcccttc tcgagcctcg caaccacgtc cttccgttcg
     1801 gcggtcgagc ggaagcggtt gaggtagtcg gcttctacgc caaattcctt gaggcggtcg
     1861 gagaaggttt tgtggtgttg gagcgccagg atggtggtgg gacaaagaat ggccacttgt
     1921 tttttgtcta aaatggcttt gaaggcagcg cgcacggcca cttcggtttt gccaaagccc
     1981 acatcgccgc agatgaggcg gtccatgggg tagtctttga gcatgtcgtc tttgacctcg
     2041 ttggtggctt tgagctggtc gggcgtgtct tcgtagataa aggaggcttc gagttcggtt
     2101 tgcaggtagc cgtcgggcgc gaaggcgaaa ccgggcgagg ctttgcgttt ggcgtagagc
     2161 tgtatgagtt ctttggcaat gtctttgacc ttctttttgg tcttggcttt gaggtttttc
     2221 caggcttcgg agccgagttt gttcacctgc ggctcggtgc tgtctttgcc ggcgtatttg
     2281 gctattttgt gcagcgagtt gatgctgacg tagaggatgt cgttgttttt gtaaatgagg
     2341 cgcacggctt cctggatatg gccgttgatg ttgatttttt caaggccgga gtacttgccg
     2401 atgccgtggt ctatgtgcgt cacgaagtcg ccgggttgca actcgttgag catgcgcacg
     2461 ttgagggcca tttctttggt gtagccctgt ttgagtttgt agcgatggaa gcgctcgaaa
     2521 atctggtggt cggtgtagca ggccactttg aggtcgttgt ccacgaagcc ttcgtgtatg
     2581 gcgcagatga ccgggtggaa cttcacatcg gccttgaggt cttcaaaaat ggagtaaaag
     2641 cgctctacct gccgggggtt gtcggtgaag aggtagttga caatgccgtt gcgggcgttt
     2701 ttttggaggt tgtcaatgag gaggttgaac tgcttgttga aggccggctg cggattggaa
     2761 tggtaagcca cgatgcgatt tacggtcaga ggctgtcgct cttctgtgcc cgtcagtacg
     2821 agggagcatt tcagcacggc ttcggcgacg gtatcgggcg tcagaaaatg cccttcgcgg
     2881 aacagcacgg cgaggtcgct ttcgtcgagt ttggaaatct ccggcgtttt ttgctccact
     2941 ctttcggcac agtgcagcag ttgcgccttc atcatctcca tgtctctgat ccacagcacc
     3001 ctatcgggtg gaagcacctc gaagaagggc actttttgag taggactgaa cttggaatga
     3061 atgttgggta cgatggaaac ccggctgatg ttttgcaccg ataattgcgt gagcggatta
     3121 aaagtgcgga tgctttctac ctcatcgtca aagagttcga tgcggtacgg ctggtcgttt
     3181 ccgaaagaaa agatgtccac gatgccgccc cggatggaaa actgccccgg ctcaaacacg
     3241 aagtcttcgc gggcaaaacc atgctctacc agcacatcaa tgatggtgtc cacgtccaag
     3301 cgctcgcctt tgttgatgtc aatgcgcgac tgatcgaggg cggcgggagc cactacgcgc
     3361 tccagcaggg cttcggggta ggtgacgacg atgaggccgg ccttgtcggt ggtggcgatg
     3421 cggttcacgg tgtcggcgcg ttccagggtt tgggtgctgt tgatttcttc aaaatgggcg
     3481 ggccgtttga aggaatcggg aaaaaagagc accggttttt gctctaataa tccggccagg
     3541 tcattttgca gataggccgc ctgctctttg tcggaagcga taagaacgtg ggtgcgcggc
     3601 tgttcgagga aggctgcggc gacggccatg gcctgttgcg cgccggtgat gccgttgagg
     3661 aggacgcggg ccgggctgtg ttgttgcagc gcttcgagga ggtttcgatt gcggcggtcc
     3721 ttgcggaaga gatcgagaag ttggtgtagg ttgctcacgg gcgcaaaggt agagatttcg
     3781 gcattattga aaaacaggga atagttaccg cattttcaca aaaaaatacc cccaatttca
     3841 cctttggtct ccgccaaaat ttaaggatat aaataattga taatcagata ttaaacaaaa
     3901 aacaatcgca acaatcttat tttcagtcaa ggcaaaaact cagcgccttg cccttgaaca
     3961 gcgcctacgc cggacgtaac tttgcatcgt tgttcaatca cagcatattc accaataaaa
     4021 ttgcaacgtc atgatcggca ccaaaaatta tttcgacacc atcatcgcca accaacagca
     4081 actgatggcc accttcaccg aatataccaa caaggctgtg gaagccatga cgcccaataa
     4141 ggaagtggcc gaaaaaggcg ccgctctgat gaatgaaatg atgacccgcc cgtatgaatt
     4201 ggccggcgaa atggccaagc gcgaaacgct ggaaaaattt caggcagact tttggggcac
     4261 ttactccgaa catctgaaca aaagcaccga tttggccgcc gatttgtacc gcaagagctt
     4321 cgagtacatg aaggaaatgt ggggcaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnncccg ccaatgggga gaagcctctc aaaatgtggc caaagtgtat aaaaacacgc
     4441 tggaagccaa tgccttagct acgaaagagt atatgaagtt ttaagccgcg agctttgagc
     4501 tgcgagcttc gagtcggatt tcccctgaga aaatgatttt ccctttcggc aaaatttcgg
     4561 cattttttca aaaaggagga acacactcca acagtcagca aaatcaggct catagctcga
     4621 agctcgtagc ccgcagctat tctaaaatgt ttttactacc acaaaaaaaa taccgccatg
     4681 aaaaacgcat gggattccgt tttggacaat cagaaaaaag tcatggattt ctggggcaat
     4741 atggctcagc aaatgacgcc caacactaaa aaggcagagg cgcccgccga tatgttcacg
     4801 caatggatgg agcagcaaaa atccgtttgg gagcagtgga tgaaaggcat gaagcccgaa
     4861 gacgctatga cggccatgcc ggaacaactg cgccaatggg ccgaaatgca gcaaaaattc
     4921 annnnnnnnn nnnnnnnntg tggaaatgta ccgcgaaaat gccggcaaaa tgggtttggg
     4981 aatgccgcag atggacatga gcgcctttat gcccaaccgc atgatggagg aaaatgtcaa
     5041 aaactggaaa aactggaccc aaagcagcga aaagtggatg cgcgatgccg tgatggccaa
     5101 aatgcctttc aacatgcagc cgcactattc caatttcatc gagttttttg acgatgtgca
     5161 gcgctattgg gagccgtttt cccgcatgat tcagaacggt attttcgaca aggcgatggt
     5221 ggaaaaatac ttcgaccaga atgcctacct caaagtggtg aaccagttga tgggtttcaa
     5281 acccgtaggc annnnnnnnn nnnnnnnggc aatgtgaccg aactcattga ttcggtgaac
     5341 cattactttg aaaaaatgat gggccagatg ggcgacgagg cctccaaatg gaacaccgta
     5401 agcgacacct ggcgctccaa aatgtcggaa gttttttccg gcagccatgc cccgcttctc
     5461 cagttcggca acgacatcaa caaccgcctg cgcgaccaac tgacgccgtt tttcaacatc
     5521 gccgctcagg gccgcgaaac ggaaatcgcc aagctcatgc gcgacgtgca gttcagctac
     5581 atcagcttca ttctgaagac ctccgaactg caatccaaag tgtatgacgc cggccagtac
     5641 gtcctccccg acctactgcg cgcctactac gaagactacc agaagacgaa agaaatgacg
     5701 gattttcagg cgtttctgca caaatacatc aacgagctgg aaaacagcat cctcaccgta
     5761 ttgaacgccg atgagtactc caagctccaa agcgaaattg ccgcgctggg tgcttccatc
     5821 aaactcaaat cggacaaggc caccgaattg gtgctcgccg acatgccctt cctcacccgc
     5881 agcgaaggcg atgatttcgc tcaggaagct gctgccctgc gcaaaaaagt gcgctctttg
     5941 gagcaacgct tagccgcatt ggagcaacac ttgggcgctg aggcggaggt agtagcagcg
     6001 gaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acggtgaaga aataatggat
     6061 tcttttttca gaatgcggcg cctgccgaac ctgtcgggcg ccgcgttttt tcaaaaacgc
     6121 aaaaccaaat aacataatga gccacgccaa agcattgagc gaacaaatca tcgagcgggt
     6181 ggacaccgcc gccgaagcac tcaaaaacat caacgatatt caggatgtgg acatcgccac
     6241 cgcacaacgc gaggtagtgt ggaatgacgg caaactgaag ctctaccact tccaccgcga
     6301 aggcgaagcc aaggctcaca cgcccatgct ggtttcttac gcattggtca atcgctggga
     6361 aatgatggac ctccaaccca atcgcagctt catccgcaag ctcatcagcg agggcattga
     6421 cgtgtatctc atagactggg gctacccgac caaaattgac cgctacaaaa ccctcgaaga
     6481 ctatgtgctg ggccatatca acgactgcgt ggacgtggtg cgccaacgtc acaatctcca
     6541 aaaaatcaat ctgttgggcg tgtgccaggg cggcactttc agcctgatgt acagtgcttt
     6601 gtttcccgaa aaaatcaaaa acctcatcac gctcgtcacg ccggttgact tcgacaacga
     6661 agaaggtctg ctcttcaaat gggccaaaga catggacgtg gacgcagtgg tggacggctt
     6721 tggaggcatc attcccggcg attttctcaa taccggtttc gacctcctca agcccatgaa
     6781 caaaacgcgc aagtacatgg ccctgcccga tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn ncgcacggtg gacctgagca agatcaacat gcccatcctc
     7021 accatttacg ccaaagacga ccacattgtg ccgccggcta ctaccaagcc gatcaacgat
     7081 ctggtttcat ccaaagacaa agaattgatg gagtttcccg gcggtcacat cggggtgttc
     7141 gtgggcgctc gttcgcaaga cttgctcacg cccgccatcg ctaagtggtt agtggagagg
     7201 gattaggcac tggttggaac ggctgccggc nnnnnnnnnn nactggctgc aagctgctgg
     7261 ctgcaagcta ctggctcaag ctactggctg caagccgcgc ttagcaccgc ccactgccta
     7321 ccgcccaccg cctaccgcct actgcatacc gcctactgca taccgaaaaa tatattacct
     7381 ggttttattt ggttagggct gccggctgca ttcaaaatgg atgcgccggc ttttttttat
     7441 accttgcgcc gccaggtttt gtgcatagcc ttcggcgcaa gttgttgatt agcagcgccc
     7501 ggtatcacac ctgatggtgc gaggtatata tagccctatc gcacccacac ttttcgcacc
     7561 aagctccgca ggccgtcgcc ggcacgcaac aggtacatac ccggtggcag atgcgccagg
     7621 ctaagcgaca tcgcgtcggc agtctgatgc tctaaacggg catcatgcac cacccgcccc
     7681 agcagatcgg tcaacgagac gttcaactgc gctccccagg agccttccgg ctgaaggaaa
     7741 agcaggtcat gggtcggatt gggaaaaagg tgcaaatcca cggtctcctc cggcttgatg
     7801 cgggcggcga ccacctgaga aaaagtacct gttccgtcgg cgtccacctg tcggaggcga
     7861 tagtaatagc tcagaccggg gcgcacatcg gcgtcttcgt gcgcataatc ggaacgaaga
     7921 tcggcatcca actttccggg cgcccaggcg atggttttaa attccatttc ccagccgtcg
     7981 cggcgttgca cctcaaatcc tgcattgttg cgctcttgcg cagtagccca gcgcagctgc
     8041 acgcttgatt tttcggcctc cgcactgaat gacaggagtg aaaccggtag cgccgactgg
     8101 cattgacaca gcaccacgtc gtccaaatac cagccgtccc aaccattgca gccgtcttcg
     8161 ccgagttccc agcgcagttg cacattatcg ccgggcgcaa tgcctatggc ggcaaggttg
     8221 atctggcttt ggccccaact gcccgctacc gaaccttcat tggctgccgt aaaagccgtc
     8281 tgcccggcta agggattgtc gttgcctgcg cccaccgttt gcagcgagct gttgtaggca
     8341 ttaaaaacaa aagcggaagc cggcagcagc gtccaggcgc cgccgttttt gctgtatttg
     8401 aggttgccgc cgtcccaggt gctttccaag gagacgtaat gctcgaagct gaggcgaaga
     8461 gtagtggccg ctgtgggcgg aatggaaatg accgggcttt gcagacgctg aatgccgttt
     8521 tccagatcgc tgctgcaatt gcccacataa gcgttggccg cactgatgcc ggtaccgggg
     8581 cggctgcgag gcagggagga gttgagttgc caggtgcggg ccgtccaggt ggccgggttg
     8641 gtggga
//
LOCUS       DAORLF010000183         8622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4439_length_8622_cov_2.198669, whole genome shotgun sequence.
ACCESSION   DAORLF010000183 DAORLF010000000
VERSION     DAORLF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_8622_cov_2.198669"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK198_14495"
     CDS             complement(<1..439)
                     /locus_tag="PK198_14495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF39996.1"
                     /translation="MISFECKTFNELANSELYEVMSLRQEVFVVEQKCFYQDADGKDP
                     QAWHLLGADAEGDLVAYARIFPRGAVYAEYPAIGRILTSMKVRGTGAGRLLVAEAIRH
                     CERLCGKLPIKIGAQAHLDRFYGEFGFHMVGEPFLEDGIPHIYM"
     gene            complement(436..>1785)
                     /locus_tag="PK198_14500"
     CDS             complement(436..>1785)
                     /locus_tag="PK198_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF39997.1"
                     /translation="AKSARRGDWIVGRGWHQEKWTKPIEKAVLGYPYHDSLSAISPDN
                     PVILSHASGHSLFANAKAMELAGITADTPNPPGGEIVRDARGTAIGVFEERAMSLITK
                     AYDAYLASLSSEQKKEQWLKGIELAEQECLSKGITSFQDAGSSFQEMEWYRELAEAGR
                     MKVRLWAMVRHPYHTLKGKIDAYPVIDAGNGYFTCRAIKSELDGALGAYGAWLLAPYD
                     DKPGFSGQNTTPIEEVRRIAGLALERNMQLCVHTIGDKANRELLNLCEELFRSQPNAK
                     DLRWRSEHAQHIDPVDIPRFPQIGIIAAMQGIHCTSDAPFVVKRLGEERARTGAYPWR
                     SLLDAGAVVTNGTDAPVEDVSALESFYASVTRKRADTGMAFFPEQRMTRAEGVYSYTL
                     ANAYAAFEEKNKGSIEAGKLADFVVLSNDLLNCPDEDILSTRVLMTIVGGKVKFKAE"
     assembly_gap    1786..1797
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1798..2160)
                     /locus_tag="PK198_14505"
     CDS             complement(<1798..2160)
                     /locus_tag="PK198_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF39998.1"
                     /translation="MKYFLPLLTLAALFLAASCGRPQPAETADLILINGNIYTVDSLQ
                     MSAQAIAVKGERILKVGSNEDIQQLKGDSTQVIDLEGKFLMPGFIEGHGHYAGLGSSL
                     MNLNFLKSKSWDDIVKLVE"
     gene            complement(2174..2800)
                     /locus_tag="PK198_14510"
     CDS             complement(2174..2800)
                     /locus_tag="PK198_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF39999.1"
                     /translation="MPDEQRRKPPTREETMQRLAAQWNMQYEPKDDWGLVKKLGDFKL
                     FRTGGSKRITNIVKNKDAVLESAIYLFDYKYTVSTGKSSSTYKQTVFFVDSKQLGLPQ
                     FSMKPENFLHWIGDLLGFKDIDFEDYPEFSNNYFLKGEDEEYIRHALPHQFLRFFSDN
                     KEKAWYLEGINYFLIFYQADHLVGPRELKSFYEKGLRIYQMLKTDFQP"
     gene            complement(2800..3843)
                     /locus_tag="PK198_14515"
     CDS             complement(2800..3843)
                     /locus_tag="PK198_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40000.1"
                     /translation="MKKILVIEDNAEVRENLEEILELSGYEVLTAEDGKVGVEIALKE
                     LPDLVLCDVMMPKLDGFGVLNILSKKSSTADIPFVFLTAKTEKSDFRRGMNLGADDYV
                     TKPFYKDELLTVIETRLRKSEQIRKKFDRTENGLSAFINEAKGYEELKKLSLERRIKH
                     YKKREVIFEEGDYPRYLYFVNSGKVKVFKTNEDGKEYIIDIFMDGDFVGYNDLIRDTK
                     YAEGASALEDTDISLIPREDFVALLHANRDVASQLIKMLANNVTEKEEQLLNLAYHSV
                     RKRVANAILLLSEKEGDAEINILRDDLARIVGTAKESVIRMLTEFKEDGYIEINDGAI
                     VLKNKHKLENLPG"
     gene            complement(3868..5541)
                     /locus_tag="PK198_14520"
     CDS             complement(3868..5541)
                     /locus_tag="PK198_14520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF40001.1"
                     /translation="MDAVPPRPGVFNADMRLKAVFEAALDGIVTISERGIIEMANPAA
                     ARMFGYEDAGELIGRNISMLMPQQHAAQHDHYIQRYLDTGHKRIIGIGREVPGLRKDG
                     SVFPLRLSVSEIKLEGRSMFTGIVHDLTLEKKAALELQQEKERAQQYLDIANTIIVVI
                     SSLGIVELLNDKGCEVLGYAESDVLGQDWFQTAIPAELQDETRSVFRRMMNGEMAILD
                     YYESDIVTRNGDRRLIAWRNAPIYDDAGAIRASISSGVDITEQRAAEDRIIRLNAELE
                     QRVDQRTEELAGAVNQLLNINKKLEHEIQERRAVETALRNSEQELRRAYEKEKELSEL
                     KSRFVSMASHEFRTPLSTILSSADLIEAYTREEQQEKRAKHTGRIKSSVSNLTGILND
                     FLSLSKLEEGKVGVQPGLFRLLPFLETTADELSGLLKPGQKIYKHLPEHDLEMFSDQK
                     VLKNILFNLLSNAIKYSEAGSRIDCTVEPEGDRLRISIKDEGIGIPQEEQQHVFTRFF
                     RAHNAENIQGTGLGLNIVKRYVELLGGSINFESELGKGTTFVCLLPIKM"
     gene            5705..>6739
                     /locus_tag="PK198_14525"
     CDS             5705..>6739
                     /locus_tag="PK198_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF40002.1"
                     /translation="MARLEILDVGSNKGKALKANAEEALHELGMDLPIEDVSDISRLL
                     EYGISGIPALVVDGRVVLQKMVPSAKELRVMLSILLSSPTGSHSLTLRSIVAPTDFSE
                     ISFNALAYAKSIAAVQSAGLSLVHVHQAQAIAPGPFPVPASQEDVEWKNGQLDSFLGR
                     PVYTNGFESGRVPMRKELLIGPVVEEIRRLSELSDSDMIVMGSTGKTNLLNRLFGSVS
                     SEIARRASCPVLLVPAEARFRGFRQIIFAGNYEPHEERVLPRLLSFARRFDSQVQYVH
                     VNTNAEQEYVVNKIPLLPVGESRQNISELTTIESSDILDGLLRYAAEQKADLIAMSTT
                     QRSFMEELFHR"
     assembly_gap    6740..6764
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6856..7254
                     /locus_tag="PK198_14530"
     CDS             6856..7254
                     /locus_tag="PK198_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HRF40003.1"
                     /translation="METGFFVLFDGVCNLCNGTVQFIIRKDRRGRFRFAALQSEAGRQ
                     LLQQYQLPEKALNTVVLIAKGRAYTRSAAALEIARRLDGAWPICYAAIALPRFFRDAV
                     YNFIARNRYRWFGKQEACMMPSPELKQRFL"
     gene            complement(7260..>8622)
                     /locus_tag="PK198_14535"
     CDS             complement(7260..>8622)
                     /locus_tag="PK198_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40004.1"
                     /translation="ASFSLPATSDNGIAGSWSPAINNTATTTYTFTPNAAAHPCAVTT
                     TMTVQVTPLPTATANPAEICAGQSATLSASGGSFYQWSTGASGPSVSVTPSSTTTYSV
                     TVTTEGCSSVATATVTVHPLPVPAIARSADVICTGGTATLTASGGNTYQWSTGEQTPV
                     IVVSPTVTAMYSVTATDQRGCTASASTSVQVNALPLVQLSADNLGCYGERRGRLEIEQ
                     ISGGLPPYSYAIGSEAWRSVGALPATAAADLAPGNYQFRVRDSGNCLNTTSFVIPEAP
                     QLQLDLGPDPIIRIGDSLLLEGLANFVIDSVVWSPDTLLSDPTAAATFARPLTTTGFS
                     LTAFDRNGCRVSDQVIVYVQRVVDVFVPNAFSPNGDGVNDKLVVLTERKIQHIRSFSI
                     FSRWGEEVFFVSDFPPNDPTYGWDGTHRGSALNAGVFVYYLEVVYPDGGVELLKGDVL
                     LMR"
BASE COUNT         1818 a         2277 c         2371 g         2119 t
ORIGIN      
        1 ccatatatat atgcggtatg ccgtcttcca gaaaaggctc gcctaccatg tggaacccga
       61 attcgccgta aaaacggtcc aaatgcgcct gcgcccctat tttgatgggc agctttccgc
      121 acaggcgctc acaatgccgg atggcttcgg ccaccaaaag ccgtccggcg ccggtacccc
      181 gcactttcat actcgtgagg atgcgcccga tggccggata ctcggcataa accgccccgc
      241 gaggaaagat gcgcgcatac gccaccagat cgccctccgc atcggcgccc agcaggtgcc
      301 aagcctgggg gtctttgccg tcggcatcct gatagaagca cttctgctct accacaaaca
      361 cctcctgccg cagggacatc acttcataca actcgctgtt cgccagttca ttgaaggttt
      421 tacattcaaa agaaatcatt cggctttgaa ttttactttg cctcccacga tggtcatcag
      481 cacgcgggtg gagaggatgt cttcgtcggg gcaattgagc agatcgttgg acagcaccac
      541 gaaatcagcc agcttgccgg cttcgatgga acctttgttt ttttcttcaa aagcggcata
      601 agcattggcc agtgtgtagg aataaacccc ctcggcgcgg gtcatgcgtt gctccgggaa
      661 gaaggccatg cccgtatcgg cgcgtttgcg ggtcacggag gcatagaagc tctccaatgc
      721 gctgacgtct tccacaggag cgtcggtgcc gttggtcact acggcgccgg catccaagag
      781 gctgcgccag gggtaggcac cggtgcgggc gcgttcttcg ccgaggcgtt tgaccacaaa
      841 aggggcgtcg gaggtgcagt gtatgccctg catggcggcg atgatgccga tttgaggaaa
      901 gcgcggaata tccacaggat caatgtgttg ggcgtgttcg ctgcgccagc ggaggtcttt
      961 ggcattgggc tgcgagcgga acaactcttc gcaaagattg agcagttcgc ggttggcttt
     1021 gtcgccgatg gtgtgtacgc agagttgcat attgcgctca agcgccaggc cggcgatgcg
     1081 gcgcacctct tcgatggggg tggtgttttg gccgctgaaa ccgggcttgt catcgtaggg
     1141 cgcgaggagc caggcgccgt aagctccgag tgcgccgtcg agttccgatt tgatggcgcg
     1201 gcaggtgaag tagccgttgc ctgcatcaat gacggggtag gcatcgattt tacccttgag
     1261 ggtgtgatag ggatggcgca ccatggccca gaggcgcacc ttcatacggc cggcctcggc
     1321 gagttcgcgg taccattcca tttcctgaaa cgaggagccg gcatcctgaa acgaggtgat
     1381 gcctttggag aggcattcct gttcggccaa ttcgatgccc ttgagccact gttctttctt
     1441 ttgttcggaa gaaagcgagg ccagataagc atcgtaagcc ttggtaatga gactcatagc
     1501 gcgttcttca aaaacaccga tggccgtgcc ccgcgcatcg cgcacgatct cgccgccggg
     1561 tggattgggc gtatcggccg taatgcccgc cagctccatc gctttggcat tggcaaacaa
     1621 agagtggccg ctggcatggc tgaggatgac ggggttgtcg ggcgatatgg cgctcaacga
     1681 atcgtggtag gggtagccga gcactgcttt ttcgatgggt ttggtccatt tttcctggtg
     1741 ccagccccgg cccacgatcc agtcgccgcg gcgggcggat ttggcnnnnn nnnnnnnttc
     1801 caccaatttt acaatatcat cccagctttt gcttttcagg aaattgaggt tcatcaggct
     1861 gctgccgagg cccgcatagt ggccgtgccc ttcgatgaag ccgggcatga ggaatttgcc
     1921 ttcgaggtca atgacctgcg tgctgtcgcc cttcagttgt tggatgtcct cattggaacc
     1981 gactttgagg atgcgctcgc ctttgacggc aatggcctga gcgctcattt gcagggagtc
     2041 aacggtgtag atgttcccgt tgatgaggat gagatcggcc gtttcggcag gttgaggacg
     2101 accacaggat gccgccagaa aaagcgcggc gagggtgagg agcgggagga agtatttcac
     2161 gatggatgat tttttacggc tgaaaatccg ttttcagcat ctgatagatg cgcaggcctt
     2221 tttcataaaa gcttttcagt tcgcgtggcc cgaccaaatg atcggcctga taaaaaatca
     2281 gaaaatagtt gatgccctct aagtaccagg ccttttcctt gttgtcggaa aagaagcgca
     2341 gaaattggtg tggaagggcg tggcggatgt attcttcgtc ttcgcctttg aggaagtagt
     2401 tgttggaaaa ctccggatag tcttcaaaat caatgtcttt gaaacccagc aggtcgccga
     2461 tccagtggag gaaattttcg ggcttcatcg aaaactgcgg cagacccaat tgtttggaat
     2521 ccacaaaaaa gacagtctgt ttgtaggtac tggaagactt gccggtgctg acggtgtatt
     2581 tgtaatcgaa caaataaatg gccgattcca aaactgcgtc tttgttcttg acgatgttgg
     2641 tgatgcgttt gctgccgccg gtgcgaaaga gtttgaaatc gccgagtttt ttcaccaagc
     2701 cccagtcgtc tttcggctcg tattgcatgt tccactgggc agccagccgc tgcatggttt
     2761 cctcgcgggt aggcggtttt cggcgttgtt cgtcaggcat tatccgggca aattttcgag
     2821 tttgtgtttg tttttgagga caatggcgcc gtcgttgatt tcgatgtagc cgtcttcttt
     2881 aaactcggtg agcatgcgaa tgacactctc tttggcggtg cctacgatgc gtgccagatc
     2941 gtcgcggagg atgttgattt ctgcgtcgcc ttctttttcg ctgaggagaa gaatggcgtt
     3001 ggccacgcgt ttgcgcaccg agtggtaggc cagattgagg agttgctctt ctttttcggt
     3061 gacgttgttg gccagcattt tgataagttg ggaggccacg tcgcggttgg cgtgcaagag
     3121 cgccacaaag tcctcgcgcg gaatgaggct gatatcggtg tcttccaacg ccgaagcgcc
     3181 ctctgcgtat ttggtatcgc gaatgaggtc gttgtagccc acaaaatcgc cgtccataaa
     3241 gatgtcaatg atgtactcct tgccgtcttc attggtttta aacaccttga ccttgccgga
     3301 attgacaaaa tagaggtagc gcgggtaatc gccttcttcg aagatgacct cgcgtttttt
     3361 gtaatgcttg atgcggcgtt ccagcgagag ttttttgagt tcttcgtagc ctttggcctc
     3421 gttgataaag gcgctgaggc cattttcggt gcgatcaaat tttttgcgga tttgctcgct
     3481 tttgcgcagg cgggtttcga tgacggtcag cagttcgtct ttgtaaaacg gcttggtcac
     3541 atagtcgtcg gcgcccagat tcatgccgcg cctgaaatcg cttttttcag ttttggccgt
     3601 cagaaacaca aagggaatat cggcggtgct gcttttttta ctcaaaatat tgagcacgcc
     3661 gaatccgtcc aatttgggca tcatcacatc acacagcacc agatcgggca gttctttcag
     3721 cgcaatctcc acgcccactt tgccgtcttc ggcagtgagc acctcataac cagacaattc
     3781 cagtatttct tccagatttt cgcgtacctc ggcgttgtct tcaatgacta agattttttt
     3841 catactgctt tattttttta tcgcccctta catttttatc ggcaacaagc acacaaaagt
     3901 agtgcctttg cccaattcgc tctcaaaatt aatactccct cccaataatt ccacatatcg
     3961 ctttacaatg ttcaggccca ggcccgttcc ctgtatgttt tcggcgttgt gcgcgcggaa
     4021 aaagcgggta aaaacgtgtt gctgttcctc ttgcggaatg ccgatgccct cgtctttgat
     4081 ggagatgcgg agccggtcgc cttctggttc cactgtgcag tctatgcggc tgccggcctc
     4141 ggagtatttg atggcgttgg agaggaggtt gaataggatg tttttgagta ctttttgatc
     4201 agaaaacatc tctaaatcat gctcaggcaa gtgtttgtaa attttctggc ccggcttgag
     4261 cagtcctgaa agctcgtcgg cagtggtttc caaaaaagga agcagcctga aaaggcccgg
     4321 ctgaacccct acttttcctt cttccaattt gctcagagag aggaaatcgt tgaggatgcc
     4381 ggtgaggttg gacaccgagg acttgatgcg gccggtgtgt ttggcgcgtt tttcctgctg
     4441 ctcttcgcgg gtgtaggctt cgatgagatc ggccgaagac aggatggtgc tgaggggggt
     4501 gcgaaactcg tgcgaggcca tagaaacgaa gcgggacttg agttcgctca gttctttttc
     4561 tttttcgtaa gcgcggcgca gttcctgttc gctgttgcgc agggcggttt cgacggctcg
     4621 gcgttcctga atttcgtgtt cgagtttttt gttgatgttg agcagttggt tcaccgcgcc
     4681 ggccaattcc tcggtgcgtt gatctacgcg ttgttccaat tcggcattca ggcggatgat
     4741 gcggtcttcg gcggcacgct gctccgtaat gtccacgccg gaactgatgg atgcgcgaat
     4801 ggcgccggca tcgtcataaa tgggggcatt gcgccaggca atgagacgcc gatcgccgtt
     4861 gcgcgtgacg atatcgcttt cgtaatagtc gagaatggcc atttcgccgt tcatcatgcg
     4921 cctgaaaaca ctgcgggttt catcttgtag ttcggcggga atggctgttt ggaaccagtc
     4981 ttgccccagc acatccgatt cggcgtagcc cagtacttcg cagcctttgt cattgagcaa
     5041 ttccacgatg ccaaggctgc tgatgactac aataatggta ttggcaatgt ctaaatattg
     5101 ctgggcgcgt tctttttcct gttgtagttc aagggcggcc tttttttcca aagtgaggtc
     5161 gtgtacaatg ccggtgaaca tgctgcgccc ctccaacttt atctcgctca cactgagccg
     5221 caaaggaaaa acagaaccgt ctttgcgcaa tcctggtact tcccggccaa tgccgatgat
     5281 gcgcttatgg ccggtgtcaa ggtagcgctg gatgtagtgg tcatgctgcg ctgcatgttg
     5341 ttgcggcatg agcatggaaa tgttccggcc tatgagttcg ccggcgtctt catacccaaa
     5401 catacgggct gctgccgggt tggccatttc gatgatcccc cgctcactga tcgtaacaat
     5461 gccgtctaaa gcggcttcaa acactgcctt gagccgcata tcggcattaa aaacacccgg
     5521 acggggagga acagcatcca caggtaaatg acttttatga gttatcaaat gcttaaactg
     5581 accaaaatta tcacgacgaa tgacaaaagt cattatttcc ctccgcaaat cagcgaatct
     5641 tgcagcatta ttttgcggat tataagctcc gttcagtttt cagtcacttt aaaccatcta
     5701 ctgcatggcc cgtcttgaaa ttttggatgt ggggtcaaac aaaggtaagg ctctgaaagc
     5761 caatgcagaa gaagcgctgc atgaattggg tatggacctg cctatcgaag atgtgagcga
     5821 cataagccgc ttgctggaat acggcatttc cggcattccg gcattggtgg ttgacggtcg
     5881 ggtggtactt caaaaaatgg tgccctccgc caaagaattg cgcgtgatgc tcagcatatt
     5941 actgtcttcg cccacaggga gccactccct caccttgcgc agcatcgtgg cgcctaccga
     6001 tttttccgaa atttctttca atgcgctggc ttacgccaaa agcattgccg ccgtacaaag
     6061 cgccggcctg agtctggtgc atgtgcatca ggcgcaagcc attgccccgg gaccatttcc
     6121 cgtaccggct tcgcaagagg atgttgaatg gaaaaatggg cagttggaca gttttctggg
     6181 gcgaccggta tataccaacg gcttcgaatc cgggcgggtg cccatgcgca aggagctcct
     6241 catagggccg gtagtggagg aaatccgccg tttgtctgag ctttccgaca gcgatatgat
     6301 tgtgatgggc agcaccggca aaaccaactt gctcaatcgc cttttcggca gcgtttcttc
     6361 cgaaatagcc cgcagagcct cctgtccggt gttgttggtt ccggcggaag cccgattcag
     6421 aggtttccgg cagatcattt ttgccggcaa ttatgagccg cacgaagagc gcgtactgcc
     6481 ccgcctgctg tccttcgccc gccgtttcga cagtcaggtg cagtatgtac acgtcaacac
     6541 caatgcggaa caagaatatg tggtgaacaa aatccccctg ctgccggtgg gcgagagccg
     6601 tcaaaatatt tcagaactca ccaccataga gagcagcgat attctggacg gactgctgcg
     6661 ctatgctgcc gaacaaaaag ccgatctcat cgccatgtcc accacgcaac gctcctttat
     6721 ggaggagttg tttcaccgcn nnnnnnnnnn nnnnnnnnnn nnnncaaata cccctgctca
     6781 tcatgcaccc ggaagatatg cgggaagccc gctcttagcg tttcaacaat aataggtccc
     6841 gcacgtttat ccaacatgga aaccggattt ttcgtgcttt tcgacggcgt ttgcaatttg
     6901 tgcaacggca cggttcagtt catcatacgc aaggaccggc gcgggcgctt tcgttttgcc
     6961 gccctgcaat cggaggccgg ccgccaattg ctccagcaat accaattgcc tgaaaaggcg
     7021 ctcaacacag tagtactcat agccaagggg cgcgcctaca cccgctctgc cgccgcgctc
     7081 gaaatagccc gccgcctcga cggagcctgg ccgatatgtt atgcagccat cgcattgcct
     7141 cgttttttca gagacgcggt ttacaatttc atcgcccgca accggtatcg ctggtttgga
     7201 aaacaggagg cctgcatgat gccttcgccg gagttgaaac agcggttttt gtgatccggc
     7261 tatctcatca acagcacatc tcctttcaat aactccacac cgccgtcggg atataccact
     7321 tctaaataat agacaaacac gcccgcattc aaggcgctgc cccggtgcgt gccgtcccag
     7381 ccgtaagtgg ggtcattggg cggaaaatcg gatacaaaaa acacctcttc tccccagcga
     7441 ctgaagatgc tgaacgatcg gatatgctgg attttgcgct ccgtcaacac gaccaattta
     7501 tcgttcacgc cgtcgccgtt aggactgaac gcattgggta caaacacatc caccacgcgc
     7561 tgcacataca cgatgacctg atcggacacg cggcagccgt tgcggtcaaa tgccgtcaga
     7621 gaaaaaccgg tcgtcgtcag cggcctcgca aaagtagcgg cagcggtcgg atcggaaagc
     7681 agcgtatccg gcgaccaaac aacgctgtct attacaaaat tggccaaacc ttccaacaac
     7741 aggctgtctc cgatgcgaat gatgggatca ggccccaaat cgagctgcaa ttgcggcgcc
     7801 tccggaatca caaaagatgt ggtattcagg cagttgccgc tgtcccgcac ccgaaactgg
     7861 tagttgcccg gcgctaaatc ggcggctgcc gttgccggaa gagcgcccac acttcgccag
     7921 gcttcactgc cgatggcata gctgtacggt ggcagtcctc cgctgatctg ctcaatttcc
     7981 aatctgcctc tgcgctcgcc ataacacccc aaattgtctg ccgacaactg caccaaaggc
     8041 agcgcgttca cctgtacgga ggtgcttgcc gatgccgtgc agcccctttg atcggtcgcc
     8101 gtcacgctgt acatcgctgt gactgtggga gatacgacaa tgacaggcgt ttgctctccg
     8161 gtactccatt ggtaagtatt tccgcccgat gccgtcaagg ttgccgtgcc gccggtgcaa
     8221 atcacgtctg ccgaacgggc aattgccgga acgggcaacg gatgtaccgt caccgtagca
     8281 gtggccaccg acgagcaccc ttccgtagta accgtaacgg aataagtcgt tgtgctactt
     8341 ggcgttacgc tgacagacgg cccgcttgcg ccggtactcc actggtaaaa actgccaccc
     8401 gaagcactca atgtggcgct ctgtccggca caaatctccg caggattagc cgtagccgtc
     8461 ggcaagggcg tcacctgcac ggtcatggtc gttgtcactg cacagggatg agcggcggcg
     8521 ttcggtgtaa aggtgtaagt cgttgttgcc gtgttgttta tcgctggcga ccagcttccc
     8581 gcaatgccgt tgtcggacgt tgccggaagg ctgaaactcg ct
//
LOCUS       DAORLF010000184         8564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4465_length_8564_cov_2.183923, whole genome shotgun sequence.
ACCESSION   DAORLF010000184 DAORLF010000000
VERSION     DAORLF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_8564_cov_2.183923"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK198_14540"
     CDS             complement(<1..600)
                     /locus_tag="PK198_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRF40005.1"
                     /translation="MEKIDLTKLYKNYYTAKPTPGLIQVERAVFVSITGKGDPSEESF
                     SADIQALYPVAYGVKAMCKAADRDFGVPKLEGLWWFDEEKFGSPSMDEAPVKIPRSEW
                     EYRLLIRMPDFVDEAMVAQAVQTVVAKKKMPGAARVHFFEMTEGPCVQIMHIGPFSEE
                     PATLQKLNQFMQEKGMLKNGLHHEIYLSDFRATAPEKLKT"
     gene            complement(714..1466)
                     /locus_tag="PK198_14545"
     CDS             complement(714..1466)
                     /locus_tag="PK198_14545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF40006.1"
                     /translation="MYQLTTADMEILEQALNDQLSEEAIVALQRRLSEDPGFKTEAEH
                     FLTTYAAMRTVRRERWRSHLKQQSSARHSRRSFPFTRRRWIWAAAAALALAGIAALLC
                     PPASRQQSAAVLAAQEALDVPIWRGATMSEAAADPLRAQAGTAYNARRYKEAAGFLRS
                     YFEQHPGPDADLELMLGVALLHAEQPDEALEILTRRKSNPDADPYETDWYRALALLLL
                     QRGEEARSLLQSLSEAPNAYQSEAEKLMRKEF"
     gene            complement(1492..2100)
                     /locus_tag="PK198_14550"
     CDS             complement(1492..2100)
                     /locus_tag="PK198_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF40007.1"
                     /translation="MNLRLAIYFYHHFLFINTALMTLADFLEKLWTDGATAVLEKVYH
                     EDRKVCVRNLIKHRLAKKEAAEDVFMDALIVLLENAGQGKIQPSATKVSTYLIQVCRN
                     MILSLHEKNEKERLFRLLGIQPNTETDEQFEHTIQLLKKCLALLKDAAKELVDHWLED
                     LPHREIAQRMGYANANTSKARFWQITRSLGRCIEQHRKETDN"
     gene            complement(2357..2857)
                     /locus_tag="PK198_14555"
     CDS             complement(2357..2857)
                     /locus_tag="PK198_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRF40008.1"
                     /translation="MTTQEGKEKFIQSWGALGTSWGINRTMAQIHALLLISPQPLCAD
                     EIMEALKISRGNTNMNLRALMDWALVYKELKTGERREFFVAEKDMWQVVRNIILNRKK
                     KELEPTLRVLDELSSVEGNDPETEEFTRIIKEIRLFSNKADSLLESLTKADPGWLMNS
                     FIKLIK"
     gene            complement(2871..2990)
                     /locus_tag="PK198_14560"
     CDS             complement(2871..2990)
                     /locus_tag="PK198_14560"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRF40009.1"
                     /translation="MWKEDDRSLSAEFTFKDFSQAFAFMTEVALLAEKHAHHV"
     gene            complement(3045..4316)
                     /locus_tag="PK198_14565"
     CDS             complement(3045..4316)
                     /locus_tag="PK198_14565"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRF40010.1"
                     /translation="MAVDVLLGLQWGDEGKGKVVDFLAPGYDVVARFQGGPNAGHTLV
                     FGGRKYVLHTIPSGIFRDNIINLIGNGVVIDPITFAKEIDMLRQAGVPYSERLRLARK
                     AHLILPSHRYLDAATEAAKGKARIGSTLKGIGPTYMDKTGRNGLRVGDITSAGFEEKY
                     QSLKSKHLTLLKLYPEIDFNLEQEEARWMESLDVMRNIVMVDGEYYINELLEQDKNVL
                     AEGAQGSMLDIDFGTYPFVTSSNTITAGVCTGLGIAPSRVAEVIGITKAYCTRVGSGP
                     FPTEQDNETGEFLRREGGEFGATTGRPRRCGWLDLPQLRYTAMLNGVTQLAVTKIDVL
                     NNVEQILAATHYRYDGQETHQLPYDVDATPIEPVFRQFPGWKQSLAGIDRYENLPPAA
                     ADYLRFIESELNIPIAMASTGPERESLIVRR"
     gene            complement(4545..5573)
                     /locus_tag="PK198_14570"
     CDS             complement(4545..5573)
                     /locus_tag="PK198_14570"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF40011.1"
                     /translation="MKKPKQSSNKRGGWVLAVVAAFLLLYWGNLLRQSLPAQYGEDPQ
                     ELAGFVRWLLGYVAVAAALAGLGWLNFKGPFRYLSERRQRRLYQAWMLVLWWLLFELL
                     QWRTDYDPDVVEENLLVSALGWAFTAGFTIVLDAVRARRERVELVQQKTAAELHSLRA
                     QLNPHFLFNALNTLYSEAIQQGQEKLSWLIGELSGILRFSLQQAQQDEVDIAEEITFL
                     QRYIHLQEARLPENPQRRLDFQFNWDERPAQVAPLLLLPFVENAFLYGLHPSRPCFLN
                     LQLDADQQRLVFSIENSIIRPQVHRGAGLGMANSRQRLEHLYPGSHRLKVETSEDVFK
                     VRLEIELR"
     gene            complement(5545..>5817)
                     /locus_tag="PK198_14575"
     CDS             complement(5545..>5817)
                     /locus_tag="PK198_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40012.1"
                     /translation="VVERFQSRTYFSVQVPAGLVQLRTTGRPKYFVTEKNFRIEAQAG
                     QEYYLEAVLDYDFMSGSLYLVQRSAEDFKKRMKGLKLNEKAKTKLE"
     assembly_gap    5818..5880
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5993..6778)
                     /locus_tag="PK198_14580"
     CDS             complement(5993..6778)
                     /locus_tag="PK198_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40013.1"
                     /translation="MQLLRAFLLPAALFALTAMQAQNLEIVNDSVAAPPDPERYDKSI
                     YVNLSAGPSNIGLKTGYRNMHQLLKDQNVIFFKDNSQYTMGFGGRFQRWYLDVQIAST
                     FNVLGEYPANSVRYLEAESDYFNVGVNVGYAFLQTRNDVWVVRGGIGMSEYYMRITEV
                     APTVGFDFNNLSGNPSWRAWPAFSHLSAVGNVSLEWQQGGRAKRGVSLAPGFVVGYQF
                     GLGQSRWKLSDATVFNAPADRAGFAYFGVDFKIARNFERKPKN"
     gene            complement(6916..>7377)
                     /locus_tag="PK198_14585"
     CDS             complement(6916..>7377)
                     /locus_tag="PK198_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRF40014.1"
                     /translation="LSEHPAIPAARGRLEWAVEQFELCRRLSGLETGKGACFHFHLKK
                     CRGACAGQESAADYNERAQQAAERLQTGFEEDFLLMERGRTPQERAVVLVQDGQLAGY
                     GYVETSEGLSLEELLEAVRHIPGNPETTRIIQGYLHRRPVGLKRLDLNEKR"
     assembly_gap    7378..7389
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7390..8329)
                     /locus_tag="PK198_14590"
     CDS             complement(<7390..8329)
                     /locus_tag="PK198_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRF40015.1"
                     /translation="MSAERYAIIDIETTGGRADLHRITEIAVILHDGERILDTYETLL
                     NPETYIPYGITELTGITQDMVKDAPRFFEVAKKIVEMTEGAVFVAHNASFDYGFIREE
                     FARLGYTYSRQQLCTVRLSRKAFPGLRSYGLDNLIATFGIEVKQRHRAMGDALATAEL
                     FRRIMAQNGGPDQALSMIKMGLKDARLPENFGIERLMALPEACGVYYFHDERGDVLYV
                     GKSQNIRKRIAEHFADKTEKTRKLRQMTHDITCEVTGSELVALLLESFEIKRLRPSIN
                     RAQRQQTFPFAVYAQPDDAGYLCFHVERITATQRKKL"
     gene            8359..>8564
                     /locus_tag="PK198_14595"
     CDS             8359..>8564
                     /locus_tag="PK198_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40016.1"
                     /translation="MKLLLAFIATISIACGTKNVPTSIAPAPVPAVEAPSATPLLCGG
                     GGLPERWCLVASGQLVRNSSSPARG"
BASE COUNT         1890 a         2232 c         2306 g         2061 t
ORIGIN      
        1 ggttttcagt ttttcgggcg cggtagctct gaaatcggac aggtaaattt cgtggtgcag
       61 gccgtttttc agcattccct tttcctgcat gaattgattc aatttttgca gcgtagccgg
      121 ctcctcagag aatgggccga tgtgcatgat ctgaacgcat ggcccctcgg tcatttcaaa
      181 aaaatgcacc cgtgcagcgc cgggcatttt ctttttagcc accaccgttt gcacagcttg
      241 ggccaccatc gcttcgtcca caaaatcggg catccgaatg agcaggcggt attcccactc
      301 gctgcggggt atttttacgg gcgcttcgtc catagacggc gaaccgaatt tttcctcgtc
      361 aaaccaccat agcccctcca acttaggcac gccgaagtcc cggtcggcag ctttgcacat
      421 tgccttcacg ccataagcca ccgggtaaag cgcctgaata tcagcagaaa aggactcttc
      481 ggaaggatcg cctttgccgg ttatcgaaac aaagacggcg cgttctacct gaatcaggcc
      541 cggcgtaggc ttggcggtgt agtagttttt gtatagtttg gtcagatcaa ttttttccat
      601 ttttgccctt ttgagttcag ggcaaaaata gccaatattt cgaacatttt atcaggcaac
      661 atgcggccta aagcaactca cggctcgtag cttgcaggca tcccctgaaa aaactaaaac
      721 tccttcctca tcagtttctc cgcctccgac tgataggcat taggcgcctc gctcagagat
      781 tggagcaagg agcgggcttc ttccccgcgt tggagcagga gcagcgccaa tgcgcggtac
      841 cagtcggttt catacggatc ggcgtcggga ttcgattttc gtcgggtcag tatctccaag
      901 gcttcgtcgg gttgttcggc gtgcagcaag gccactccca gcatcagttc gagatcggcg
      961 tcgggaccgg ggtgttgttc aaaatacgag cgcagaaaac cggcagcttc tttgtaccgg
     1021 cgtgcattgt aggccgtgcc tgcctgggcg cgaagcgggt cggcagccgc ctcgctcatc
     1081 gtagcgccgc gccatatcgg aacatccagc gcctcttggg cagccaacac cgcggcagac
     1141 tgctgccggg aagcgggagg gcaaagcaga gcggctatgc cggccaaagc caatgcggca
     1201 gcggcggccc atatccagcg gcgacgagta aaggggaagc ttcgccggct gtgcctcgcg
     1261 gatgattgct gtttcaggtg ggagcgccag cgctcgcggc ggacagtgcg catggcagcg
     1321 taggtagtta aaaaatgttc ggcttctgtc ttgaaaccgg ggtcttccga cagcctccgt
     1381 tggagtgcta caatggcttc ctcgctcaat tggtcgttca gggcctgttc gaggatttcc
     1441 atgtctgcgg tggtaagctg gtacatgttt taggagttaa agtgttgtag atcagttatc
     1501 tgtttctttc ctgtgctgct caatgcaccg gcctaatgat cgcgtgattt gccagaaacg
     1561 ggctttggag gtgttggcat tggcatagcc cattcgttgg gcaatttccc gatgaggcag
     1621 gtcttccagc cagtgatcaa cgagttcttt ggcggcatct ttgagcaaag ccaggcattt
     1681 tttcagcaat tgtatcgtat gctcaaactg ctcgtcggtc tcggtattcg gttgtattcc
     1741 cagtaagcgg aacagacgct ctttttcatt tttttcatgc aggctcaata tcatgttgcg
     1801 acatacctga atgaggtagg tcgaaacttt tgtggcggag ggctgaatct ttccttgccc
     1861 ggcattttcc agcaaaacga tgagggcatc cataaaaaca tcctcggctg cttccttttt
     1921 cgccagacgg tgtttgatca aattcctgac acagaccttc cgatcctcat gatagacttt
     1981 ttccaacact gctgttgcgc cgtcagtcca tagtttttcc aaaaaatcgg ccaaagtcat
     2041 aagtgctgta ttgatgaata agaaatgatg gtaaaagtaa atcgcgagcc tgagattcaa
     2101 tgagcacttt tttgcaaaaa agtgctcccc acagatttac tttttgcctt ttctggtata
     2161 aaccttctgt gaaaagcccg atgataacaa cgtattattt ggggggcatg tatcatattg
     2221 aaataccggt gcctaaaatt caagccaata tgaaaaccaa aacaatttac acagatttcg
     2281 gtattgagaa atatttgatg ccccaacccc gcctgaaata acaggcaata agctaagccc
     2341 cagtgtttta cgatctttat ttaatcaatt tgataaacga gttcatcagc cagccgggat
     2401 cggccttggt gagggattcg agcagggagt cggccttgtt ggaaaacagc ctgatttctt
     2461 tgatgatgcg cgtaaactcc tcggtttcag ggtcattacc ttctacgctg gagagttcgt
     2521 ccaatacgcg gagggtgggt tccaattctt ttttcttgcg attgaggatg atgttgcgca
     2581 ccacttgcca catgtctttt tcggccacaa aaaactcgcg gcgctcgccg gttttcagtt
     2641 ctttataaac caatgcccag tccatcagcg cccgcaggtt catgttggta tttcccctcg
     2701 aaattttgag cgcctccatg atctcatcgg cgcagagcgg ctgcggagaa atgagcaaca
     2761 gggcatgaat ctgagccatg gtgcgattga tgccccaact ggtgccgagg gcgccccagg
     2821 actggatgaa tttttctttg ccttcttgcg tggtcatgcc tcagttagtt ttatacatgg
     2881 tgggcatgct tttcagccaa cagcgccacc tcggtcataa aagcgaatgc ctgcgagaaa
     2941 tctttgaatg taaattccgc cgataaagag cggtcgtctt ccttccacat gtcagcaact
     3001 attgagcagc tcgcagcttg tagctcgcgg cctgaagcca gatactacct cctcacaatc
     3061 aaactctccc tttccgggcc ggtagaggcc atggcgatgg gaatgttgag ttccgattcg
     3121 atgaagcgca ggtagtcggc ggcagcgggc ggcaggtttt cgtagcgatc tatgcctgcc
     3181 agcgattgct tccaacccgg aaactgccgg aacacaggct cgatgggcgt agcatccacg
     3241 tcgtagggca gttgatgggt ttcctgtccg tcatagcggt agtgggtggc ggccagaatc
     3301 tgctccacgt tgttgagcac gtcaattttg gtgacggcca attgcgtgac gccgttgagc
     3361 atggctgtat aacgaagttg aggcagatcg agccagccac agcgccgggg cctaccggta
     3421 gtagcgccga actcgccccc ctcgcggcgc aaaaactcgc cggtttcgtt gtcttgttcg
     3481 gtaggaaacg gccccgaacc cacgcgggtg cagtaggctt tggtgatgcc gatgacctcg
     3541 gccacgcggg agggcgcgat gcccagcccg gtgcaaacgc cggcggtgat ggtattggaa
     3601 gaggtgacaa aagggtaagt gccgaagtca atgtcgagca tggagccctg agcgccctca
     3661 gccagcacgt ttttgtcttg ttccagcaac tcattgatat aatactcgcc gtccaccata
     3721 acgatattgc gcatcacgtc gaggctttcc atccagcggg cttcttcctg ttcgaggttg
     3781 aagtcaattt caggatagag cttgagcaag gtgaggtgtt tagacttcag agactgatat
     3841 ttttcttcaa aaccggcgga ggtaatatcg cccacgcgga gaccgttgcg ccccgttttg
     3901 tccatgtagg tggggccgat gcccttgagg gtagaaccga tgcgggcctt gcctttggcg
     3961 gcctcggtag cggcgtccaa atagcggtga gagggcaaaa tgagatgggc tttgcgggcc
     4021 aatcgcaggc gttcggaata aggcacaccg gcctgccgga gcatgtctat ttctttggca
     4081 aaagtgatgg gatcaatgac gacgccgttg ccgatgaggt tgatgatgtt gtcgcggaaa
     4141 atcccggaag gaatggtgtg caaaacatat ttgcgcccgc cgaaaaccag cgtgtgcccg
     4201 gcattggggc caccctgaaa ccgtgccacc acgtcatatc ccggcgccag aaagtccaca
     4261 actttgccct tgccttcgtc gccccattgg aggcccagga gaacatctac tgccataagt
     4321 ttttgataat tagttttttg agattgaaaa aacaaaacat tccgacttta tgccggaatg
     4381 tttattcaga cctcaaaggt aggaaaaagt ttctggtttg cgggttggaa cgctgatttc
     4441 taattacggg ttggaactgg tgtatttcgg atttttaatc acaggttggg gcaggcttgt
     4501 ttcggatttc aggttgggac cgcccgcccc gcgtcatggc gcggctaccg aagctcaatc
     4561 tccagcctca ctttaaaaac atcctccgaa gtctccactt tgaggcgatg gctaccgggg
     4621 tacaagtgct ctaagcgttg gcggctgttg gccatgccca atccggcgcc gcgatgtacc
     4681 tgcggacgga tgatgctgtt ttcgatagaa aaaaccagcc gctgctgatc tgcgtcaagt
     4741 tgcaaattca ggaaacatgg gcgggaaggg tgcaagccat ataaaaaagc attttccaca
     4801 aatggcagaa gcaagagcgg cgccacctgc gcaggccgct catcccagtt gaactgaaaa
     4861 tcgaggcggc gctgcgggtt ctccggcagg cgggcctcct gaaggtggat gtagcgctga
     4921 agaaaggtga tttcctcggc gatgtccacc tcgtcttgtt gggcttgttg cagggagaat
     4981 cgcaggatgc ccgacagctc gccgatgagc cacgacagct tttcctggcc ctgctggatg
     5041 gcctcgctgt acagggtatt gagggcgttg aagaggaaat gtgggttcag ttgtgcgcgc
     5101 aggctgtgca gttcggcggc ggttttttgc tgcacgagtt ctacgcgttc gcggcgggcg
     5161 cgcaccgcat ccaagacgat ggtaaacccg gccgtaaagg cccagcccag cgctgaaacg
     5221 agcaggtttt cctccactac atcggggtcg tagtcggtgc gccattgcag gagttcaaac
     5281 aaaagccacc acaataccag catccaggct tgatagaggc ggcgttgccg acgctcgctg
     5341 aggtagcgaa acggcccttt gaaattgagc cagccaagtc cggccagcgc ggccgcaacg
     5401 gccacatagc ccagcagcca acgcacaaaa cctgccaact cctgtgggtc ttcgccgtat
     5461 tgagcaggca ggctttggcg cagcaaattt ccccaatata agagcaaaaa tgcagccacg
     5521 acggccaaca cccaaccgcc tcttttattc gagctttgtt ttggcttttt cattcaattt
     5581 caagcctttc atgcgttttt tgaagtcctc ggcactgcgt tgtaccaaat acaacgaccc
     5641 cgacatgaag tcgtaatcca gcacggcctc aagatagtac tcctgcccgg cttgcgcctc
     5701 tatgcggaag tttttttccg tcacaaaata tttcggccgg ccggtcgtgc gcagttgcac
     5761 aagacccgca ggcacttgca cgctgaagta agtgcggctc tgaaagcgtt caaccacnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 ttggtaaata tacaacacag catgtccggg cgccatattt tgtgccatga tgaattgcgt
     5941 acttcccgac actatgaaaa gcactgcaaa aatgagtgtg cgatacatgg cctcagtttt
     6001 ttggtttgcg ttcaaaatta cgtgcaatct taaaatccac accgaaatag gcgaatcctg
     6061 cgcgatcggc cggagcattg aacacggtag cgtcgctgag tttccatctg gattggccca
     6121 gcccgaactg atagccgacg acaaagcccg gcgccagcga gacgccccgt ttggcccggc
     6181 cgccctgctg ccattccaaa ctgacattgc ccactgcgct gaggtgcgaa aatgcgggcc
     6241 aggcgcgcca ggatgggttt cctgataagt tgttgaaatc aaagcctaca gtaggagcca
     6301 cctctgtgat gcgcatatag tattcgctca tacctattcc gccgcgcacc acccacacat
     6361 cgttgcgggt ttgtaagaaa gcgtatccca cattaacgcc cacattaaaa taatcggatt
     6421 cggcctccag atagcggacg gaattggctg gatactcgcc cagtacattg aatgtggaag
     6481 ctatttgtac atctaaatac cagcgttgaa aacgccctcc gaagcccatc gtgtactggc
     6541 tgttgtcttt aaaaaaaatg acgttctgat ctttaagtaa ctggtgcatg ttgcggtaac
     6601 ctgtttttaa tcctatattg gacggaccgg cactcagatt gacataaata ctcttgtcgt
     6661 atcgctccgg gtcgggcgga gcggccactg aatcgttgac gatttctaag ttttgggcct
     6721 gcatggctgt cagcgcgaag agggcagccg gcagcagaaa agcgcgtagt agttgcataa
     6781 gcggtaattt tttgatgaag aatgccgcaa gataaccccg tcgctcgccg cttgtcaaat
     6841 ggaagtggga agtggcacag aatatgtgat gaatggtgcg tttggtgtga gcatggaccg
     6901 gagatgcagc gttctttacc tcttttcgtt caaatccagc ctcttcagcc ccaccggcct
     6961 gcgatgcaga tagccctgaa tgatgcgcgt ggtttccggg tttccgggga tatgtctaac
     7021 cgcctccaac aactcttcca ggctcagtcc ttccgaggtt tctacatagc cgtaacctgc
     7081 caactgcccg tcttgcacca gcaccacggc gcgttcctgc ggcgtgcggc cgcgctccat
     7141 cagcagaaaa tcctcctcaa aaccggtttg gagtcgttcg gcggcttgct gcgcgcgttc
     7201 gttgtagtcg gcggcagatt cctgacctgc gcaggcgccc ctgcattttt tgagatgaaa
     7261 atgaaaacag gcgcccttgc ccgtttccaa tccgctcaag cggcggcaca gttcaaactg
     7321 ctctacggcc cactccaagc gacctctggc ggcaggaatg gccgggtgtt cagacaannn
     7381 nnnnnnnnng gagttttttg cgctgtgtgg ctgtaatgcg ctccacatga aagcacaggt
     7441 atccggcatc gtccggctgg gcatacacgg caaaagggaa ggtttgctgg cgttgtgcgc
     7501 ggttgatgga ggggcgcaat cgcttgattt caaaggactc cagcaagagc gccaccaatt
     7561 cgctgcccgt cacttcgcag gtgatgtcgt gggtcatttg gcgcagcttg cgggtcttct
     7621 cggttttatc ggcaaagtgt tcggctatgc gtttgcgtat gttctggctt ttgcccacat
     7681 acagcacatc gccgcgctca tcgtgaaaat aatacacgcc gcaggcttcg ggcagggcca
     7741 tgagccgttc tatgccgaag ttttcgggca ggcgggcatc tttcagaccc atttttatca
     7801 tggagagggc ctggtcgggg ccgccgtttt gggccatgat gcgccggaac agctcggcgg
     7861 tggccaatgc gtcgcccata gcgcggtggc gttgtttcac ttcgatgccg aaggtggcaa
     7921 tgaggttgtc caaaccataa gaacgcagtc cggggaaggc tttccggctc agccgcacgg
     7981 tacacaattg ctggcgcgag taggtataac ccaagcgggc aaactcctcc cggatgaagc
     8041 cataatcaaa agaggcattg tgcgccacaa ataccgcccc ctccgtcatt tccactattt
     8101 ttttggccac ctcgaaaaag cggggcgcat ctttcaccat atcctgtgtg ataccggtca
     8161 gttccgtaat gccgtaagga atgtaggttt cggggttgag taaggtttcg taagtatcca
     8221 aaatgcgctc gccatcgtgc aaaatgacgg caatttccgt gatgcggtgc aaatctgcac
     8281 gccctccggt ggtctcaata tcaataatgg cgtatctttc agcggacatt ccgcaaattt
     8341 aaaacaaaaa atatgaactt gaaattattg cttgctttca tcgcaaccat ttccatcgcc
     8401 tgtggaacga aaaacgtgcc gacatccatc gctccggcgc ctgtgcctgc cgtagaagcg
     8461 ccgtcggcaa cgcctttgct ttgcggcggc ggaggtttgc cagagcgatg gtgcttagtc
     8521 gcatcgggac aactcgtcag aaatagttct tcgcccgctc gagg
//
LOCUS       DAORLF010000185         8547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4478_length_8547_cov_2.235516, whole genome shotgun sequence.
ACCESSION   DAORLF010000185 DAORLF010000000
VERSION     DAORLF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_8547_cov_2.235516"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    210..353
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <354..658
                     /locus_tag="PK198_14600"
     CDS             <354..658
                     /locus_tag="PK198_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40017.1"
                     /translation="KKLSPFFELGVAPSMYLTSRKKTVTDIGKESEVPNGDIQNFNRF
                     HIVGFISFGLNYSLNDQFQLFGQPVFRYHFTPLADAPISEYLLNYGIEIGIRRKIN"
     gene            821..2515
                     /locus_tag="PK198_14605"
     CDS             821..2515
                     /locus_tag="PK198_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRF40018.1"
                     /translation="MRSTKTIIAVIFAIFHQLTYAQVDVIYNNLVWSDEFEVNGAINS
                     AKWHHQTQIPAGGSWYNGEVQHYTNLLSNSFVENGVLKIVARKQTFADQGVTKEYTSA
                     RLNSKFAFTYGRVDIRAKIPTNQGTWPALWLLGKNITEPGGYFAATHGTTPWPACGEI
                     DIMEHGITPWQPAGFIQSALHTPSSFGSTVNHGGTIANNLGTDFHVYSMNWSPNQITF
                     LLDGVAFYTYNPAVKNASTWPFIADQYLLLNIAMGGVAGNIPGNFTQASMEIDYVRVY
                     QNTTPDTQAPANFTATLGTVTSSTVQLLVNAQDNSGTVVYNVAYGDQNVQVTELSGVQ
                     KSIVIPNLSPNTLYTFSVTASDFAGNMAANNPIVIDATTPAVIGCFGAGTSAQQGSFS
                     TGYHYIFETLGNDVKITFTLLDTDRVGVVAFLWRQTPFTEYQMTALGNNAFTYTITGQ
                     TPGANISYGVKFAYAGGLSVTTYYNYQVGNNCVVGLGNITTDSDFYFQNPCNGILEIQ
                     SQNSINKVEIYDINGNLVLKTRQANQPISLQHLPKGVYFIRVYYRDRTQTKKLIKI"
     gene            complement(2569..>3856)
                     /locus_tag="PK198_14610"
     CDS             complement(2569..>3856)
                     /locus_tag="PK198_14610"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF40019.1"
                     /translation="NTIVWSEGFGYADVEKGIRATPETQFRIASVSKLLAAAVVGKMH
                     EQGRVDIDADVRKYVPEWPDKCDKITIRQLAGHLGGIREYRPTDFTVKNIDGKRYKNA
                     KEALSIFADDTLVAPPGTKHNYTSLGYTLMEAALEGASGESYIRYMKKHILDPLRMTS
                     TLPNHPDSTIARLTTLYTRDSKGHNKTIRNMKPTYKFAGGGYVSTAEDLARFGMAHLR
                     PGLFKASTLELLFQTQYLNTGEPTSVGIGWVNDRDPWGRRIVFHNGNQPGARPVLIVY
                     PDHDMVIAITSNVTGIPEWVEGAAMCIADPFLRLTAGEKPLPVPDSIGTFDVVMKKDE
                     ADAFSGSMTLLNPAETPENGYRGWMDALPGSNLSVWPIADVIAWPTETVALAATPKGL
                     FPMRWQKNADGSSSGFFTFFGEHRPETLPVYLTKME"
     assembly_gap    3857..3866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3867..4068)
                     /locus_tag="PK198_14615"
     CDS             complement(<3867..4068)
                     /locus_tag="PK198_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40020.1"
                     /translation="MVKITWPNFFKVFAPLLSIVFRPIPVEAQVRYIRQPEYLTAIAH
                     GRIALKTMRETADIPGLAVAVGI"
     gene            complement(4062..>4255)
                     /locus_tag="PK198_14620"
     CDS             complement(4062..>4255)
                     /locus_tag="PK198_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40021.1"
                     /translation="NIEKTVEYELFDIAGRLVQRGKADNPGVFLSIDTGARLAKGAYV
                     LRLRESGGTFETFKLAGGW"
     assembly_gap    4256..4279
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4280..7697)
                     /locus_tag="PK198_14625"
     CDS             complement(<4280..7697)
                     /locus_tag="PK198_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF40022.1"
                     /translation="MKQLATYFYTFVSAALAIGIALAPFSEQQKQAPVHRKALSIRQA
                     LEDNFERTKDPALGYPPTERLHAAIEYARRLQLQMSSREKGPDNLPDALWRERGPDNF
                     SGRTRAILIDQNDPSRKTIWAGSVAGGLWKCSDISADPPQWEIVSDYLENMAVGALAQ
                     DPVDPQVMYMGTGEAFNNLDAVRGRGIYRSDDGGATWTALAATLVANFRFTRDLFVHP
                     ETRDVYAATTQGLMRSQNRGESWARVVGIPANLANSSIYDIQYSPSTEMIYISNATGV
                     WRSPDGNPSTWQNISTAATGFPQNLERVEMTLSASNPDVIYIIGQVDGGASNVFTTSN
                     GGTTWTQRARPNNSNGNEFTNGQAWYDLEIAVDPFNPSHVIVGGVGIMRSTNGGVSWS
                     FFANNMHVDQHKVVFDPQQQGVVYFGNDGGFYRSTNGSSATVQNKKRNYNVTQFYACA
                     LHPEAGSDYMLGGTQDNNSLQLNSVGIGSARSVRGGDGMFCHLDQLDPNIQMVSSQNA
                     NYSLSTNGGQTFSTAVDLDGRFVCPSDYDSEAKIMYSQTESGDYYRWFVNTGVTELVD
                     VQSFELSVSCITVDPNTPGRVYFGNFSNGRIFRVNNAREGATVTGVALSPLPGTVSNI
                     DIEIGNPDHLLATVSNYGVPSVFESFNGGQNWTNVEGNLPDMPVRWGIFNPANSQQAM
                     IATEAGVWITEQLDGANTFWMPPLADIGIPLVRTDMLKVRRSDRTVLAATHGRGLYTS
                     DVFSEVSIRAVFDPITYQNVPKRFTGSLSANAVSYLWDFGDGTTSTQSDPEHAFAQPG
                     QYNVRLTINGDLSTESVVTVLPDRPLPYVPAKPEYGGGFEAFTGQYAAHTLGGSAFER
                     GKSNIAKKDGTHGGDNAFVLGLNETFYQAGTYSILYLPNYDFSENTLYDFSFWGKWEI
                     HNGFDGLRVEYSTDRGRNWQPLGNQITPGWYNFRNNNLDGAAFPVGSAYFTGNRANWE
                     EFKFNLSFLAGQPDVAFRFVFIADDNGSHAGVALDDVTIQKYEGELVTKLLNFTGSFP
                     NGENVRLDWNTQPEYYCRQFEVERSVNGRDFERIATVNATGILTTALQSYQHTSLALK
                     NLYFYRLRVINENEPTGYNHVFYSPIVVMQRRPSDALVNRVFPGPFGNTIN"
     gene            7943..>8547
                     /locus_tag="PK198_14630"
     CDS             7943..>8547
                     /locus_tag="PK198_14630"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF40023.1"
                     /translation="MLLLTAALLTTAFAQAQPYSLPKLPYDYAALEPHIDAQTMEIHY
                     SRHHQAYVTNLNKAVAGTPLEKYPLEDLLIAAGRRSAAFRNNGGGHYNHTLFWEILSP
                     QAQKAPQGALLAAINAQFGSLDSLKTALNQAASTRFGSGWAWLVVTPSKKLQVCSTAN
                     QDNPIMDVSEVRGIPVLGIDVWEHAYYLKYQNKRGDYLGAI"
BASE COUNT         1994 a         2051 c         2299 g         2025 t
ORIGIN      
        1 ttgtgggtgg aatagagtac tctttccggc atttgacgac ttcaagccag gatatttttg
       61 tacggagggt gctggatgac cgggaggctc aggaaatcgg gaagctcaac tggcgaattg
      121 gttttaatta caaccgaaaa atatccaaga acatattttt taagacaggt cttcgatttg
      181 caagcattgg atataaggga gaaaaggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggaaaaa
      361 gttgtcgcct ttttttgaat tgggagtggc gccgtcaatg tatctgacct caagaaaaaa
      421 aacggttacc gatatcggaa aagaatctga agttccgaat ggcgatatcc agaatttcaa
      481 tagatttcat atcgtaggat ttatttcatt cggattgaat tattcgctga atgatcaatt
      541 ccaacttttt ggacagccgg tattcaggta ccattttacc cctttggcag atgcgcctat
      601 cagtgaatat ttgttgaatt acggcatcga aattggaata agaaggaaaa taaactaaaa
      661 acgaactggc cgggcttggc attgcaaaaa gaaggcttag ctttgcaaca acacgcgtgc
      721 tttgaatccc tgatgtgatg gacaaagctc cgaatggctt tgaagcatca agaaggcttt
      781 tcgccggact ttggcggatg aactgcacaa ttaaataaaa atgagatcaa caaaaacgat
      841 catcgcagtc atatttgcga tatttcatca attgacgtac gctcaagttg atgttattta
      901 taataatttg gtctggtcgg acgaatttga ggtaaacgga gccatcaaca gcgccaaatg
      961 gcatcatcaa acgcaaatac ccgccggggg cagttggtat aacggtgaag tgcaacatta
     1021 taccaatcta ctttccaatt cttttgtaga aaatggcgta ttgaaaatag tggcaagaaa
     1081 acaaaccttt gcggatcaag gagtaacgaa agaatatacc tctgcgcgat taaattccaa
     1141 gtttgcattt acttatggcc gagtggacat acgcgctaaa atacctacaa atcaaggtac
     1201 ctggcccgct ttatggcttt tggggaaaaa tataacagaa cccggcggct actttgccgc
     1261 aacgcacggc accactcctt ggccggcgtg tggggaaatt gatatcatgg agcacggtat
     1321 cacgccgtgg cagccggcag gattcattca aagcgcgctc catacgcctt catctttcgg
     1381 gagtacagta aatcatggcg gcaccattgc taataattta ggaaccgatt ttcatgttta
     1441 ttccatgaat tggtcgccca atcagattac ctttttattg gatggcgtag ctttttacac
     1501 ctataatcct gctgtgaaaa atgcctccac ctggccattt attgccgatc aatatttgct
     1561 gttgaatata gctatggggg gtgttgcagg aaatataccg ggcaatttca cccaggcaag
     1621 catggaaatt gactatgtga gagtgtacca aaacacaacg ccggatacac aggctcctgc
     1681 caattttacg gcaactcttg gtacggttac aagttctacg gttcagcttt tggtaaatgc
     1741 tcaggataat tcaggaacgg ttgtttacaa tgtagcttat ggcgatcaaa acgtacaagt
     1801 tactgaatta tcaggcgtac aaaaatcaat tgtaataccc aatctttccc ccaatacttt
     1861 atatactttt agtgttacag cttcagattt tgcaggaaat atggctgcca ataatcctat
     1921 tgtgatagat gcgactactc ctgctgttat aggttgtttc ggggcgggaa cctcggctca
     1981 acagggcagt ttttcaacgg ggtatcatta tatttttgaa accctgggca atgatgtgaa
     2041 aatcactttc actttgttgg atacggacag agtgggggtt gttgcttttc tttggagaca
     2101 aacgccattt acggaatatc aaatgacggc attgggcaat aatgccttta cttacaccat
     2161 taccgggcaa acaccgggcg ccaatatcag ttatggcgta aaattcgctt atgcaggggg
     2221 gctgtctgtg accacttatt acaactatca agtgggaaat aattgtgttg tcggcttagg
     2281 aaatattact acagactccg acttttattt ccagaatcca tgcaatggaa ttttagaaat
     2341 tcaatctcaa aacagcatca ataaagttga aatttatgac atcaacggaa acttggtttt
     2401 gaaaacgagg caggcaaatc agcctatttc cttacagcat ttacccaaag gcgtttattt
     2461 tatcagagtt tattacaggg atagaaccca aacgaaaaaa ttgattaaaa tatgaacgaa
     2521 caaccaagct cgtagctcgc ggcttgtagc ttgcagccaa catcctcctc actccatctt
     2581 cgttaaataa accggcagcg tctccggccg atgctctccg aaaaacgtaa aaaagccgct
     2641 tgatgagccg tccgcatttt tttgccagcg catagggaac aagcccttgg gtgtagccgc
     2701 cagcgccacc gtttcggtag gccaggcgat cacatcggca atgggccaca cgctgagatt
     2761 gctgcccggc agcgcatcca tccaaccgcg atagccgttt tcgggcgttt cggcggggtt
     2821 cagcagggtc atggagccgg agaaggcgtc ggcctcgtct tttttcatca caacatcaaa
     2881 ggtgccgatg ctgtctggaa ccggcaatgg tttctctccg gctgtcagcc gcaaaaacgg
     2941 atcggcgatg cacatggccg cgccctccac ccattcggga atgcccgtca cgttggaagt
     3001 gatggcgatg accatgtcgt gatcgggata gacgatgagt acgggccgcg caccgggctg
     3061 attgccgttg tgaaaaacaa tgcggcggcc ccaggggtct cggtcgttca cccagccgat
     3121 gccgaccgaa gtaggctcgc cggtgttgag gtattgggtt tggaacagca gttccaacgt
     3181 agaggctttg aacaagcccg gacgcaggtg cgccatgccg aaacgcgcca ggtcttcggc
     3241 ggtagaaaca tagccgccgc ccgcaaattt gtaggtgggc ttcatgttgc ggatggtttt
     3301 gttgtgacct ttgctgtcgc gggtataaag cgtggtgagg cgcgcaatgg tgctgtcggg
     3361 gtggttgggc agggtggaag tcatgcgcag cggatcgagg atatgctttt tcatgtagcg
     3421 aatgtacgac tcgcccgatg cgccctccaa agcggcctcc atcagcgtgt agcccagcga
     3481 agtgtagttg tgtttggttc ccggcggggc caccagcgta tcgtcggcga agatgctgag
     3541 ggcttctttg gcatttttgt agcgcttgcc gtctatgttt ttgaccgtaa aatcggtggg
     3601 gcggtactcg cggatgccgc cgaggtgccc cgccaattgc cggatggtga ttttgtcgca
     3661 cttgtcgggc cattcgggca catatttgcg cacgtcggcg tctatgtcaa cgcggccctg
     3721 ttcgtgcatt ttgcccacca cggcagcggc caagagcttg gaaacactgg caatgcggaa
     3781 ttgcgtttcg ggcgttgctc gaatgccttt ttccacatcg gcatagccga aaccctcgct
     3841 ccacacaatg gtattgnnnn nnnnnncgat gcctaccgcc actgccaggc ccggaatatc
     3901 ggccgtttcg cgcatggttt tcagcgcaat gcgcccgtgg gcaatggccg tgaggtattc
     3961 aggctgccga atgtaccgaa cctgcgcctc cacaggaata gggcgaaaaa cgatgctcag
     4021 caagggagca aaaactttga aaaagttggg ccaggtaatt tttaccatcc accggctagt
     4081 ttaaaggttt caaaagtgcc gcccgattcg cgcaagcgga gcacataagc tcctttggcc
     4141 aaacgagcgc ctgtatctat gctcaaaaac acgcccggat tgtcggcttt gccgcgttgc
     4201 accaatctgc cggcgatgtc gaaaagttcg tactccactg ttttttcaat gttgcnnnnn
     4261 nnnnnnnnnn nnnnnnnnnc gttgatcgta ttgccgaaag gccccggaaa cacccggttg
     4321 accaatgcgt cggagggccg gcgttgcatc actacaatgg gcgaatagaa cacatgattg
     4381 tagccggtcg gttcattttc attgatgacg cgcaaccgat agaagtacaa gttcttcaat
     4441 gccagtgatg tatgttggta agattgcagc gcggtggtga gaataccggt ggcatttacg
     4501 gtggcgatgc gttcaaaatc gcggccgttg acagagcgtt ccacttcaaa ctgacgacaa
     4561 taatactccg gctgggtgtt ccagtccaag cgcacatttt cgccattggg gaaactcccg
     4621 gtgaagttga ggagcttggt cacgagttcg ccttcgtact tttggatggt cacatcatcg
     4681 agcgcaacgc cggcgtgact tccattgtcg tcggctatga agacgaatcg gaaggctaca
     4741 tcgggttggc cggctaagaa actgagattg aatttgaact cctcccaatt ggctctgttg
     4801 cctgtaaaat aagcgcttcc caccggaaaa gccgcaccgt ccaagttgtt gtttctgaaa
     4861 ttgtaccagc cgggagtgat ctgattgccc agcggctgcc agtttcgtcc cctgtcggtg
     4921 gaatattcta cccgcaggcc gtcgaagccg ttgtgaattt cccatttgcc ccagaaactg
     4981 aagtcgtaca gggtgttttc agaaaaatcg tagttcggca ggtacaaaat ggaataagtg
     5041 cccgcctgat aaaacgtctc gttcaggccc agcacaaaag cgttgtcgcc gccgtgcgta
     5101 ccgtcttttt tggcaatgtt cgatttgccg cgttcaaagg ccgagcctcc gagcgtatgc
     5161 gccgcatatt ggccggtgaa ggcttcaaag ccgccgccat attcaggttt tgcgggcaca
     5221 taaggcaggg gccggtcggg caacacggtc actaccgatt cggtgctcag atcgccgttg
     5281 atggtgaggc gcacgttgta ttggccgggt tgcgcgaaag cgtgttcggg atcgctttgg
     5341 gtggaggtgg ttccgtcgcc gaagtcccag aggtaagaaa ccgcattggc ggaaagggag
     5401 ccggtgaagc gtttgggcac attttggtaa gtaatcggat caaaaaccgc gcgaatgctg
     5461 acctctgaaa aaacgtcgga ggtgtaaagc ccgcggccat gtgtggcggc cagtacagtg
     5521 cggtcggagc ggcgcacttt gagcatgtcg gtgcgcacta agggaatgcc gatgtcggct
     5581 aagggcggca tccagaacgt attggcgccg tccaattgtt cggtgatcca aacgccggct
     5641 tcggtggcaa tcatggcctg ctggctgttg gccggattaa aaatgcccca tcgcacgggc
     5701 atatcgggca ggttgccttc tacgttggtc cagttctgac cgccgttgaa actctcaaaa
     5761 acacttggca cgccgtagtt ggataccgta gcgaggaggt ggtcgggatt gccgatttcg
     5821 atgtcaatat tagaaaccgt gccgggcagc ggagagagcg ccacgcctgt gacggtagcg
     5881 ccttcgcggg cgttgttcac tctgaaaatg cggccgttgc tgaagttgcc gaaatacacg
     5941 cgaccgggcg tgttggggtc cacggtgatg caggaaacac tgagttcaaa actctgcacg
     6001 tccaccaatt ctgtgacgcc ggtgttcaca aaccagcggt aataatcgcc gctctctgtt
     6061 tgagaataca tgattttggc ttcgctgtcg taatcggaag gacaaacgaa gcgcccatca
     6121 aggtcaacgg cggtagagaa ggtttgacct ccgttggtgc tgagcgagta gttggcgttt
     6181 tgggaagaca ccatttgtat gttgggatca agttggtcta ggtggcaaaa catgccgtcg
     6241 ccgccgcgta ccgagcgggc cgagcctata cctacggagt tcagttgcag cgagttattg
     6301 tcttgcgtgc cgcccagcat atagtcggag ccggcttcgg ggtgtagtgc acaggcataa
     6361 aactgagtga cgttgtagtt gcgttttttg ttttgaacag tcgccgatga gccgttggtg
     6421 ctgcgataaa acccgccgtc gttgccgaaa taaacgacgc cctgttgttg gggatcgaac
     6481 acgaccttgt gttggtcaac atgcatgttg ttggcaaaaa agctccagga tacgccgcca
     6541 ttggtagagc gcatgatgcc cacgccgccc acaatgacgt ggctgggatt gaaggggtcc
     6601 acggcaattt cgaggtcata ccaggcctga ccgttggtga attcgttgcc gttggagttg
     6661 ttggggcgcg cccgttgagt ccaggtggta ccgccgttgg aggtcgtaaa aacgttggaa
     6721 gcgccgccgt ccacttgccc aatgatataa atgacatcgg ggttggaagc gctcaaagtc
     6781 atctctacac gttccagatt ttgcggaaag ccggtagccg cagtagagat attttgccag
     6841 gtactggggt tgccgtcggg cgaacgccat acgccggtcg cattggagat ataaatcatc
     6901 tcagtagaag gggagtactg aatgtcgtag atgctgctgt tggccaggtt ggccggtatg
     6961 cccaccacgc gcgcccagga ttcgccgcgg ttttggctgc gcatgagacc ttgtgtagtg
     7021 gcggcataca catcgcgggt ttcggggtgt acaaacagat cgcgggtgaa gcggaaattg
     7081 gcaacgagcg tggcggccag cgcagtccag gtggcgccgc cgtcgtcgga gcggtagatg
     7141 cccctgcccc gcacagcatc gaggttgttg aaggcttcgc cggtgcccat gtacatgact
     7201 tgcggatcaa cggggtcttg cgccaatgcg cccacggcca tgttttcaag gtagtcgctg
     7261 acgatttccc actgcggcgg atcggccgaa atatcgctgc atttccaaag cccgcctgcc
     7321 acagagccgg cccagatggt tttccggctc gggtcgtttt gatctatgag aatagcacgg
     7381 gtgcggccgg agaagttatc ggggccgcgt tcgcgccaca gagcgtcggg gaggttgtcg
     7441 gggccttttt cgcggctcga catctgcaat tgcaggcggc gcgcatattc aatggcggca
     7501 tggaggcgct cggtaggcgg gtagccgagg gcggggtctt tggtgcgttc aaagttgtct
     7561 tccagcgcct gccggatgct gagcgctttg cggtgaacag gggcttgttt ttgttgctcg
     7621 gaaaacggcg ccaaagcaat accaatggcg agagcggcgg acacaaatgt gtagaaatag
     7681 gtagccaatt gcttcataat cgaaaatgtc ttttggtgtc gaatgatgcg gacaaatgta
     7741 acccgaaatt gcgttttgcc tgcgaaaaaa gcgaagaatg gctcctggat tgcggaatat
     7801 cgggttggat tgttggggca acccttgtgg ttggcccaac aagggttgcc ccaacgattt
     7861 ccccgacgtt tgctgatttt actacatttg caatagtttt taaatcacct taattttaaa
     7921 aataatgaaa acaacattcc gaatgctgct cctcacagcc gcattgctga cgaccgcctt
     7981 cgcacaggcc caaccgtaca gccttcccaa attgccttac gattacgctg ccctcgaacc
     8041 gcacattgat gcgcaaacga tggaaattca ctactcgcgg catcaccagg cctatgtcac
     8101 caacctcaac aaagccgtgg ccggtacgcc tttggagaag tacccgctcg aagacctgct
     8161 catcgccgcc ggacgtcgca gcgccgcttt ccgcaacaac ggcggcgggc attacaacca
     8221 cacgctgttt tgggaaatcc tctcgcccca agcccaaaag gctccgcaag gcgcgctgct
     8281 ggccgccatc aacgcacaat tcggctcttt agacagcctc aaaacggccc tcaatcaggc
     8341 cgcttctacc cgcttcggtt cgggctgggc atggctcgtg gtgacgcctt ctaaaaaatt
     8401 gcaggtttgc tccaccgcca atcaggacaa ccccatcatg gatgtctccg aggtgcgcgg
     8461 cattcctgta ctcggcattg acgtgtggga acatgcttac tacctcaaat accaaaacaa
     8521 acgcggcgac tacctcggcg ccatctg
//
LOCUS       DAORLF010000186         8443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4551_length_8443_cov_2.300787, whole genome shotgun sequence.
ACCESSION   DAORLF010000186 DAORLF010000000
VERSION     DAORLF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4551_length_8443_cov_2.300787"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    270..398
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            673..1107
                     /locus_tag="PK198_14635"
     CDS             673..1107
                     /locus_tag="PK198_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF40024.1"
                     /translation="MKNSMYALLSGRSMILSAAFLLAFVLVLSNCNTSEKKVASGEEL
                     FQSYCAICHGTNGAGNGAMTEHLKLPPADLTKIAARRGGNFPDEQIYQIIDGRVPVAG
                     HGTGDMPIWGQTLKESEQLKNEKQVRQSINNLVGYLKSIQQQ"
     gene            1172..>2342
                     /locus_tag="PK198_14640"
     CDS             1172..>2342
                     /locus_tag="PK198_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF40025.1"
                     /translation="MKKSFFLAGLLLPATFLFVSWLLPDNETWRDKVDADVLLAAESG
                     RDLEFLVVLQNQTDVSAAKLLTTKEAKGEYVFRKLRETAQNTQAPLIRLVQQSGAPYQ
                     SFFIVNALLVKGNAALLQQLAQHPATARIEPNPRTRMQEPVIDDTSESVQWRNAVEWG
                     IERIGADRVWESGYTGQGVVIGGADTGYEWNHPAIRDTYRGWNGDTVDHNYHWHDAIH
                     EIDTSNGATTNPCGLSTLEPCDDNNHGTHTMGTMTGGEDNMGSKIGVAPGARWVACRN
                     MERGDGTPATYIECFEWFLAPTDLGGNNPLPALAPHVINNSWGCPDSEGCNESNWATM
                     QMVVANLKAAGIVVVVSAGNSGPSCSSVNTPAAMFEESFSIGATRNNDTIVSFSSR"
     assembly_gap    2343..2352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2353..2942
                     /locus_tag="PK198_14645"
     CDS             <2353..2942
                     /locus_tag="PK198_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF40026.1"
                     /translation="GSGRMKPNVSAPGVGVRSSIRNGGYASFSGTSMAGPHVAGLVAL
                     LISANPELAGQVETIETIIEETALPMLSDQNCGDFSGLQIPNPVYGYGRVDAFAAVQR
                     ALELVSSTQTAAAPQQIEVFPNPFGAEINLRSHAKEQPLRLDMYNATGLLVFSRAWPQ
                     GLAFETISLHGLPSGIYLYRLHSAAGVQSGKLLKR"
     assembly_gap    2965..2991
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2992..4480
                     /locus_tag="PK198_14650"
     CDS             <2992..4480
                     /locus_tag="PK198_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40027.1"
                     /translation="DYIFQLIKAMTPAEKRYFRRHYAPEEGQLIHLFEVVNHLETYDE
                     EHLKSRLEPSVAKHLKVHKQQLHELLLKSLTSYHSKRGPLSKIRMWLEEADILAEKQL
                     FQQAFDRLAKARSLCLEYEEYTYLLEVSAREFYLKHVSNDRIGISRHPYFEETQKAIS
                     CLNAGIEYYREGTRWVEILAQYYYRPPSPEEIENALALLEREGQVNAGVLPFRARLSR
                     NSLLMSAYGLTKNQEMEGRIRKDNLALFEQSPQFQETMPFQYIGALRNLMNYCLSRQD
                     FEQARHCAQTGLSFIGRKPAFASQTIYFQYGLLDIAFQSGDWCGITGKLEQELLRTVE
                     AQALEKERIAMLCYLSLAMTYQVLNKPAVVQAYLRRAAECRNDVKEYFEELLLLLDLV
                     CHFESGDHFLVTRRIKSIRKKQENKEHPHSALLFDFVGIFTAASVEQRSRIAQDLRSK
                     MPEWQGTKLGLHIISYRVLLWLEAVAEGSTLAAYIETHNAGDRGE"
     gene            4583..6475
                     /locus_tag="PK198_14655"
     CDS             4583..6475
                     /locus_tag="PK198_14655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRF40028.1"
                     /translation="MSQVSYTEENIRSLDWREHIRMRPGMYIGKLGDGSASDDGIYVL
                     IKEVIDNSIDEFVMGFGKQIEVSVDEESVRVRDFGRGIPLGKVIDCVSQINTGGKYDS
                     KAFKKSVGLNGVGTKAVNALSEYFKVKAVREGQCKTAEFEYGELKQDHKLQKTSEPNG
                     TEVVFRPDNNIFRKYRFRQEYLENQLWNYAYLNAGLKIQFNGQTYVSKNGLLDLLTNK
                     TKADHLRYSIIHLRGEDIEISMSHGDHYGEQYYSFVNGQHTTQGGTHLNAFKEAVVET
                     INKHYGRNFDRKDILGSIMAAVSVRIEEPVFESQTKTKLGSTEIAPGGLGIRTFVGNF
                     IKEKLDNFLHKNTEIKADLLKRILQSERERKEIADIKKLANQRAKKANLHNKKLRDCR
                     LHFNDPAKKTDEDLRLATTVFITEGDSASGSITKARDVQTQAVFSLRGKPLNCYGLTK
                     KVVYENEEFNLLQHALDIEDTLDDLRYNRVVIATDADVDGMHIRLLLLTFFLQFFPDL
                     VRSGHLYILETPLFRVRDKKQAFYCYSEAEKQAAIRKLRGKAEITRFKGLGEISPGEF
                     GDFIGPNIRLEPVVLQDDTGIDAILAYYMGKNTPERQEFIIGNLRIEKDEVEAEEEVL
                     ELAAEE"
     gene            6668..7579
                     /locus_tag="PK198_14660"
     CDS             6668..7579
                     /locus_tag="PK198_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRF40029.1"
                     /translation="MKPLWGIDLGGTKIEGVVIESAEHPNVLARLRVPTEQSGGYQHI
                     IGQIQKLIGMLESATGLKPERIGIGTPGSIDPPTQLLKNCNTTALNGQPLNRDLEAAL
                     GIPVLMANDANCFAVAEARFGAVRRKVPNAQVVFGVILGTGVGGGVVVNGKVINGLQG
                     IGGEWGHNFLHESGGMCYCGRIGCVETVISGPALERYYAKQAGAKVKLAEIVARDYAG
                     EDPVAAQTLSRLLEYWGKAIAYVINILDPDAIVIGGGVGNIDLLYSEEGLAQVKKHVF
                     NPRLDTPFLKPELGDSAGVFGAALLAE"
     gene            7596..8357
                     /locus_tag="PK198_14665"
     CDS             7596..8357
                     /locus_tag="PK198_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40030.1"
                     /translation="MSIETLLYYLFLAVMALSMAQGLLIGSLFFLKKSGDRRANAFYG
                     LLLLAFGCTLAHNILTLTGMYPRHPQWYFLPIYFTLAFPPLLFYHVKFSVYPAYQFRW
                     TDLKHFVLPAAQWLFFGVMFFTDVEYKSEYGRRFFNPFFGAAEQALYLGTFIAYWYFA
                     RRYVRQRAQQTMQRGEARLVAYLRNFLNILFVLFAIHLIFVVADFVYYEFFDINLRVA
                     KPYAALGALSFAALLDWLGVYGFQVLLWGRKVFRN"
BASE COUNT         2017 a         2184 c         2190 g         1886 t
ORIGIN      
        1 acgttttttg ggagcggcag ttgtttctgc cgtttcggat gtgttgttca cctgctgttt
       61 ttgagcatct tccgatgctt cagacttttt tttcatttta aaaaatgatt tgaaaagtaa
      121 ttatttaaaa atgttttgat tctttagccg tattctattt gtagcggact gtatctaatc
      181 aattatttgt caggaactta aacaaaaaaa gaaacctgcc cgcaaacgtc atgtaccggg
      241 gtttttattg aaaaaacaaa aaataaaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt aaataatagc ggcggtttta
      421 aggaaataat ttaggattaa tttataaaat gctgcccgct ttttcctgcg ttctgagtat
      481 gtcatgcact acgccctttg tacatcaggc aataatgagc ggacaacata cctcttatga
      541 agacagggtt gccgataaaa atcatcaaaa taaaatacat ccgtcacccc ttgatgtatt
      601 caaaagactt taacttgagc aggtgaaaat ccatgcgatc attttgtaac ggtcatttaa
      661 atgaaaagca aaatgaaaaa ctcaatgtat gcgctcctat ccggcaggag tatgatctta
      721 agcgctgctt ttttgctggc ttttgtgttg gttttatcca attgcaacac ctctgaaaag
      781 aaggtggcaa gcggcgaaga attatttcaa tcctattgcg ctatttgcca cggcacaaac
      841 ggagccggca atggcgccat gaccgaacac ctgaaattgc cgccggccga tttaacgaaa
      901 attgctgcgc gccggggcgg caacttcccc gacgagcaaa tctaccagat cattgacggg
      961 cgcgttccgg tagcaggcca cggcaccggc gatatgccga tttgggggca aacgctcaaa
     1021 gaatcggaac aactgaaaaa tgaaaagcag gtacgccaaa gcatcaataa tctggtaggc
     1081 tacctcaagt ccatccagca acaataaatg gagcggcttg aaccgtagcg ccgattttgc
     1141 cgtttccgcg tggtacctaa ttgtgtgcca catgaaaaag agttttttcc ttgccgggct
     1201 gttgttgcct gctaccttcc tttttgtttc ctggctgctg cccgacaacg agacctggcg
     1261 agataaagtg gacgccgatg tgttgctggc tgcagaatcc ggcagagacc ttgagttttt
     1321 ggtagtgctt cagaatcaga ccgatgtatc ggctgctaaa ttattgacaa ccaaggaagc
     1381 caaaggagaa tacgtcttta gaaaattaag agaaacggcg caaaataccc aggcgccctt
     1441 gattcggttg gtacaacaat cgggcgcgcc ttatcaaagt tttttcatcg tcaatgccct
     1501 gttggtaaag ggaaatgccg cgttgttgca acaattggcg cagcacccgg ccacggcccg
     1561 catcgaaccc aatccccgca cccggatgca ggaacccgtc atagacgaca catctgaatc
     1621 ggtacaatgg cgaaacgccg tggagtgggg catagagcgc atcggcgccg accgcgtatg
     1681 ggaatcaggc tacaccggcc agggcgttgt cataggtggt gcggataccg gctatgaatg
     1741 gaaccacccc gccatacggg atacctaccg gggctggaac ggcgataccg ttgaccacaa
     1801 ctaccactgg cacgatgcca ttcacgaaat tgacacctcc aacggcgcca ctacaaatcc
     1861 ttgcggtttg agcactctcg aaccctgcga cgacaacaat cacggcaccc acaccatggg
     1921 taccatgaca ggaggcgagg acaatatggg cagcaagata ggcgtggcgc ccggtgcgcg
     1981 ttgggtggct tgtcgcaata tggaacgcgg tgatggcacg ccggccactt acatcgaatg
     2041 tttcgagtgg tttttggccc ccaccgacct cggcggcaat aatccgttgc ccgcgctggc
     2101 gccgcatgtg atcaacaatt cttggggttg ccccgattcg gaaggctgca acgaatccaa
     2161 ttgggcaacc atgcaaatgg tggtggccaa cctcaaagca gccggtattg tggtggtggt
     2221 ttccgccggc aacagcggcc catcgtgcag ttccgtcaac acgccggcag ccatgtttga
     2281 ggagagcttc agcattggcg ccacgcgcaa caatgatacc atcgtatcgt tcagcagtcg
     2341 cgnnnnnnnn nnacggcagc gggcgcatga aacccaatgt ttcggcgccc ggagtaggtg
     2401 tgcgctcctc cattcgcaat ggcggctatg cgtccttcag cggcaccagc atggccgggc
     2461 cgcatgtggc cggcctggta gccttgctca tttcggccaa cccggaactg gccgggcagg
     2521 tagaaaccat tgaaaccatt attgaagaaa cggcgctgcc gatgttgtcg gaccaaaatt
     2581 gtggagactt ttccggcctc caaataccca atcctgtgta tggctatggc cgggtggatg
     2641 cttttgccgc cgtgcaacgg gcattggaat tggtgagcag tacccaaaca gccgcagcgc
     2701 ctcagcagat tgaggtgttt cccaatccgt tcggcgccga aatcaacctg agaagccatg
     2761 caaaggagca gccgcttcgc ttggacatgt acaatgccac aggactgctg gttttcagtc
     2821 gcgcctggcc gcagggattg gcgtttgaaa ccatatcttt gcacggcttg ccctccggca
     2881 tataccttta ccggcttcac agcgcagccg gggtgcaaag cggcaagttg ctgaagcggt
     2941 agcgcttttc ggctgccccc ttacnnnnnn nnnnnnnnnn nnnnnnnnnn ncgattatat
     3001 cttccagctt atcaaggcca tgacgcccgc cgagaaacga tatttcaggc ggcactatgc
     3061 gcccgaagaa ggacagttga ttcatctgtt tgaggttgtc aatcatttgg aaacctatga
     3121 cgaagagcat ctgaaaagcc gtttggagcc gagtgtagcc aagcatctca aggttcataa
     3181 gcaacaactg cacgagcttt tactcaaaag ccttacctct taccacagca agcgcggccc
     3241 gctcagcaag atcagaatgt ggctggagga agccgatatt ctggctgaaa aacaactttt
     3301 ccaacaggca tttgatcgtt tggcgaaagc ccgttcgttg tgtttggaat acgaggaata
     3361 cacctatctt ttggaggtca gcgcccgcga attttatctc aaacacgtct ccaacgaccg
     3421 catcggcatc agccggcacc cttattttga agaaactcaa aaagccatct cctgcctgaa
     3481 tgccggcatc gaatattaca gagagggcac ccgctgggtc gaaatactgg cccagtacta
     3541 ctaccgcccg cccagcccgg aagaaataga aaacgccctg gccctgctcg aaagagaggg
     3601 gcaggtcaat gccggagttt tgccgtttcg agcccgattg tcccgcaatt ccttgctcat
     3661 gagcgcctac ggtctcacca aaaaccaaga gatggaaggg cgcatccgca aagacaatct
     3721 ggctttgttt gagcagtctc ctcagtttca ggaaaccatg ccgtttcagt acatcggggc
     3781 cttgcgcaat ttgatgaatt actgtttgag ccgccaggat tttgagcagg cccgtcactg
     3841 cgcccaaacg gggctgtctt ttattggccg caagccggcg tttgcctcgc agactattta
     3901 ttttcaatat ggcctgctcg atattgcctt ccaatcgggc gactggtgcg gcatcaccgg
     3961 aaaattggag caggaactgt tgcgcaccgt tgaggcacag gcattggaaa aagaacgaat
     4021 tgccatgtta tgctacctca gtctggccat gacgtatcag gttttaaaca aacctgccgt
     4081 agtgcaggcc tatttgcggc gagctgccga atgtcgaaac gatgtaaagg agtattttga
     4141 agaattgctg ctcctcttgg acctcgtttg ccatttcgag tccggcgatc attttttagt
     4201 gacccggcgc atcaaaagca tccgaaaaaa acaggaaaac aaagaacatc ctcacagtgc
     4261 cttgcttttt gattttgtcg gcatttttac agccgcctcc gtggagcaac gaagtcgcat
     4321 cgcccaagac ctgcgctcaa aaatgcccga atggcaaggg acgaaattgg gtctccacat
     4381 catcagttat cgcgttttgt tatggttgga ggcagtggcc gagggcagca cattggctgc
     4441 gtatatagaa acgcacaacg cgggggatcg cggggagtag caaccctgtg ccgggagctc
     4501 tacctgctca aaacaagtct gcatttaaaa ttttttgttt taaaaacaaa tccctacctt
     4561 tgcctctcaa agcaaaagtt ccatgtctca ggtttcttat acagaggaaa acatccgttc
     4621 attagactgg cgcgaacaca ttcgtatgcg cccgggtatg tacatcggca agttgggcga
     4681 cggctccgct tccgacgacg gcatctatgt actgatcaaa gaagtgattg acaactccat
     4741 agacgagttt gtcatgggtt tcggcaagca gatagaggtc agcgtggatg aagaatccgt
     4801 gcgcgtgcgc gatttcgggc ggggcattcc gctgggcaaa gtgattgatt gcgtgtcgca
     4861 gatcaacacc ggcggcaagt acgattccaa agccttcaaa aaatcggtgg gtctcaacgg
     4921 ggtaggcacc aaggccgtca atgcgctgtc ggaatatttc aaagtgaaag ccgtgcgcga
     4981 agggcagtgc aaaaccgccg agtttgaata cggcgagctg aaacaggatc acaaactcca
     5041 aaaaacctcg gagccgaatg gtacggaagt cgtgtttcgg cccgacaaca acatcttccg
     5101 caaataccgc ttccggcagg aatacctcga aaaccagctt tggaactacg cctacctcaa
     5161 tgcggggctg aaaattcagt tcaacggcca aacgtatgtt tccaaaaacg gcctgctcga
     5221 cctgctgacc aacaaaacca aagccgacca cctgcgctac tccatcatcc acctgcgcgg
     5281 cgaagacatc gaaatttcca tgtcgcacgg cgatcattat ggcgagcaat attactcttt
     5341 cgtcaacggc cagcacacca cccagggcgg cacccacctc aacgccttca aagaggccgt
     5401 ggtagaaacc atcaacaagc actacggccg caatttcgac cgcaaagaca tcctcggctc
     5461 catcatggct gccgtgagcg tgcgcataga agaaccggtg tttgagtcgc aaacaaaaac
     5521 caaactcggc tctaccgaaa tagcgcccgg cggcctcggt atccgcactt ttgtgggcaa
     5581 ttttatcaaa gaaaaattag acaatttcct gcacaaaaat acggagataa aagccgacct
     5641 gctcaagcgc atcctgcaat cggagcgcga gcgcaaggaa attgccgaca tcaaaaaatt
     5701 ggccaatcag cgcgccaaaa aagccaatct gcacaacaaa aaactccgcg actgccgcct
     5761 gcacttcaac gatccggcca aaaaaacgga cgaagacctg cgcctcgcca ccaccgtttt
     5821 tatcaccgag ggcgactctg cttccggctc catcaccaaa gcccgcgacg tacaaacgca
     5881 ggccgtgttc agcctccggg gcaagccgct caactgctac ggcctcacca aaaaagtggt
     5941 gtacgaaaac gaggagttca acctgctgca acatgcttta gacatcgaag acacccttga
     6001 cgacctgcgc tacaaccgcg tggtcattgc caccgatgcc gacgtggacg gtatgcacat
     6061 ccgcctgttg ttgctcactt ttttcctcca gtttttcccc gacctggtgc gcagcgggca
     6121 tttgtacatc ctcgaaacgc cgctgttccg cgtccgcgat aaaaagcagg ccttctattg
     6181 ctactccgag gcggaaaagc aggccgccat tcgcaagttg cgcggcaagg cggagatcac
     6241 ccgattcaag ggtttgggtg agatttcgcc cggcgaattc ggcgatttta tcggccccaa
     6301 catccgtttg gagcctgtgg tacttcagga tgacaccggc attgacgcca tcctggccta
     6361 ctacatgggc aaaaatacac ctgaacgcca ggagtttatc atcggcaatc tgcgcataga
     6421 aaaggacgag gtggaagcag aggaggaagt gttggagttg gcggctgagg agtagcggtt
     6481 tttttcaaaa caccagattt gctttggcca gttggcgcag gggcgtcatt gccaagatca
     6541 cagccctgcg gtcactcctt cactacgcat acgccatact aagcagtgcg ttaaaaatcg
     6601 agcaaaaagg cgcccgcttt ggccgggaaa tcctactttt atgccgatta acgaattgct
     6661 tacaattatg aaaccacttt ggggaataga cttgggcggc actaaaattg agggtgtcgt
     6721 catagaaagc gccgagcatc cgaacgtgct ggcgcgcctg cgcgtaccaa cagaacagag
     6781 cggcggctat caacacatca tcggccagat tcaaaagttg atcggtatgc tggaatcggc
     6841 taccgggctg aagcctgaac gcatcggcat cggcacgccc ggttccattg atccgccgac
     6901 acaactcctg aaaaattgca acaccacggc cctcaacggg cagccgctga acagagactt
     6961 agaggctgca ttgggcattc cggtgctgat ggccaacgac gccaattgtt ttgccgtggc
     7021 ggaagcccgc ttcggagcgg tgcgtcgcaa agtgcccaat gcccaggtgg tattcggcgt
     7081 cattctcggt accggcgtgg gcggcggcgt tgtcgtcaat ggcaaggtta tcaacggatt
     7141 gcagggcatc ggcggcgaat gggggcacaa tttcctgcac gaatcgggcg ggatgtgcta
     7201 ctgcggtcgt attggctgcg tggagacggt catttccggt ccggctttgg agcggtatta
     7261 tgccaagcag gccggcgcta aggtcaaatt ggccgagatc gtagcccgcg attatgccgg
     7321 cgaagaccct gtggcagccc aaacgctgag ccgcctgctc gaatactggg gcaaggccat
     7381 tgcttacgtc atcaatatcc tcgacccgga cgccatcgtc atcggcggcg gcgtgggcaa
     7441 tattgacctg ctctattcgg aagaagggct ggcgcaggtg aaaaaacatg tcttcaatcc
     7501 acgattggac acgcctttcc tcaagcccga attaggcgac agcgccgggg tattcggcgc
     7561 ggcattgctg gcggagtaaa cacttgcaaa tgccgatgag tatagaaacg ctgctgtact
     7621 atttgttttt ggcggtcatg gcgctgagta tggcacaggg gctgctcatc gggagtttgt
     7681 tttttctcaa aaaatcgggc gaccggcggg ccaatgcctt ttacggcttg ttgttgctgg
     7741 cctttggctg taccctggcg cacaacatcc tcacgctcac cggcatgtat ccgcgtcatc
     7801 cgcagtggta ctttttgccc atttatttca cgctggcttt tccgccgctg ttgttttacc
     7861 acgtcaaatt ttcggtgtat cccgcctatc agttccggtg gacggacctg aagcattttg
     7921 tgctgcccgc agcgcaatgg ctgttttttg gggttatgtt ttttaccgat gtggagtata
     7981 aaagcgagta cgggcggcgg ttttttaatc cctttttcgg ggcggccgaa caggcgctct
     8041 acctcggcac cttcatagcg tattggtact tcgcccggcg ctatgtgcgg caaagagcgc
     8101 agcaaactat gcagcgcggc gaggcccgat tggtggctta tttgcgcaat ttcctcaata
     8161 tcctgtttgt gctcttcgcc attcatctga tatttgtggt ggccgatttc gtgtattacg
     8221 agtttttcga catcaacctg cgggtggcca agccttatgc ggcgctgggc gcgctttctt
     8281 ttgccgcgct gctcgactgg ctgggcgtgt atggatttca ggtgctgctg tggggcagga
     8341 aggtgtttcg gaattgatgt gtggtgctcc gctggagtga ttccgctcgt gcgtgagggg
     8401 gccgagggta gtgacgacgc aggaggagcg accgaggcac ccg
//
LOCUS       DAORLF010000187         8441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4553_length_8441_cov_1.959098, whole genome shotgun sequence.
ACCESSION   DAORLF010000187 DAORLF010000000
VERSION     DAORLF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4553_length_8441_cov_1.959098"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK198_14670"
     CDS             <1..555
                     /locus_tag="PK198_14670"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRF40031.1"
                     /translation="PAVFFGLLLGDWIDSLLENVVVVGVMLVMGGIVFLMVDRWFEKA
                     ETSPDRPVSYKEALRIGLFQCLAMIPGVSRSAATIIGGLAQGLNRKTAAEFSFFLAVP
                     TMFAATAKKMLDYYQDGISLSSGEWQLLAVGNVVAFIVAMIAIKTFITFLARHGFKWF
                     GYYRIIAGAVILLLYFTGKLAWGV"
     assembly_gap    595..607
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            714..>1737
                     /locus_tag="PK198_14675"
     CDS             714..>1737
                     /locus_tag="PK198_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRF40032.1"
                     /translation="MNLNTNGKDSVTYDAIVVGTGISGGWAAKELCEKGLKTLVLERG
                     RMVKHGEYPTANMDPWDDEHRDYATQEELKDYEKQNRTGYTIRPSSKHWFVNDRENPY
                     SETKRFDWMRGYHVGGRSIMWGRQSYRLSDLDFEANAKDGIAVDWPIRYADLAPWYDH
                     VESFAGISGQAEGIPHLPDGKFLPPMEYNCLEKQVKERIESKFPGRKMIMGRTAHLTQ
                     AHNGRGNCQFRNRCIRGCPYGAYFSSNASTLPAAEKTGNMTLRPYSIVHSIIFDEAKN
                     KAVGVRVVDSETLETHEYFAKVIFLCASALGSTQILMQSTSRRFPNGFGNDSGVLGHY
                     LMDHHFR"
     assembly_gap    1738..1756
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1757..2421
                     /locus_tag="PK198_14680"
     CDS             <1757..2421
                     /locus_tag="PK198_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRF40033.1"
                     /translation="DDFQDQYYKGRRPNGIYIPRFQNLNDATKRDYLRGFGYQGGASR
                     MDWTESIREMQVGVGKDFKDSLVRPGHWEMGIMGFGECLPYHDNKVTLNKDVLDKYGL
                     PTLTMDAEFKENEMKMRKDMMFYAAEMLEAAGLRNVETYDSQSWPGLAIHEMGTARMG
                     RDPKTSVLNGWNQVHAAPNVFVTDGACMTSAACQNPSLTYMALTARAADYAVQELKKL
                     NL"
     gene            2428..3036
                     /locus_tag="PK198_14685"
     CDS             2428..3036
                     /locus_tag="PK198_14685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRF40034.1"
                     /translation="MDRREAIKKTMLITGYALTASTVQAVLTSCETEREVNWTPKYFS
                     AEQAALITNMAETILPRTKTPGAIDVGVPAFMERMVKDTYLPEDQEQFAAGLADVEAR
                     AQKAHGKPFAACKIKQQANILEALDAEASAAGIARRDAVRNNPELGAEPYFNFFLNFK
                     SLALLGYFTSSFVGTKVLSYDPIPGAYLGCIPAKEVGNAWSL"
     gene            3105..3920
                     /locus_tag="PK198_14690"
     CDS             3105..3920
                     /locus_tag="PK198_14690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF40035.1"
                     /translation="MKKSSLIVAFSLAISILFGQIEADQLYQNNEFAKAMPLFKQLSA
                     DNPGNYAHFEKYVLCLMQLQEMDQAETAMKERLKTDSTNPELYFLYGSFLSRQGRSSE
                     ADSIRQQRRKLLDEGYGAKQWAAAPPASSPSPPVKRDEIEPIKAEEADEMPLWLHPDC
                     EQVTDVKERKFCSDNKMLEHIYKNVLYPVEARENGAQGTAVITFVIEKDGSMTSLRIF
                     RDPGYGIGEAALSAVQRMQSSGIAWKPGKQKGMPVRVQFNMPVRFKLTEGGQK"
     gene            4199..4666
                     /locus_tag="PK198_14695"
     CDS             4199..4666
                     /locus_tag="PK198_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF40036.1"
                     /translation="MKKSTFFAISALFAGSLFAQTGTMPVVMEEDIINAKDADEMPLY
                     LHSECNKQTEYEARKRCADKKMLEFVYENTQYPENARTQRKEGTVVVTFVVEKDGTMT
                     DPRIARDPGYDMSEEALRVIKQMQAKGVVWKPGTKGGELVRVQFYIPIRYKLK"
     gene            complement(4669..6888)
                     /locus_tag="PK198_14700"
     CDS             complement(4669..6888)
                     /locus_tag="PK198_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF40037.1"
                     /translation="MVFSFRLGLACILIAIWWSDAIAQSTTSGFDPLYAQQLQTKLNQ
                     LGTNGNFAGGLTAAVLVPGEGIWTGAWGRAAVNTPVTTNIRFGMASNSKAIIAGLILK
                     LQDEGLLHLDDPISDYLPPHPQINPQITIRQLLEHTSGLFDFINQWLSATEAAYSANP
                     NHVWTYEELLATVGAPVSAPDAGYSYSNTNYLLLGRIAETAAGQPIHQLLHSRIFTPL
                     GLAMAYPPSDNVFAAPYSNLWNTAGTATTLTPGGSRGFLTFTSTAGSVWSTAYDMVRW
                     YDALFGHTWLSAGSRHDLTNNDGYMAYALGIRLRNIYGRSVYYHVGIWGFRSYMLHDP
                     STGISLCIIANQYGASVTTEGLELFAEALNRLPTQSHDVSIQNLAPNRTSCLPQTPLV
                     TVQNKGTATLHQLQMALGIDGEWQDTLAVSLGEGLPPGDQRLLPLAFNLGPADGQKYR
                     LQVKAILDTPDDMPANNERSAYYRFENNEAAQTDFYEDFESGGEFPGSLVSLQTGNIL
                     DWRTTRFAGHQTPGHSLARINYYDGNEDNRYAFELPLIQANTGSILSFSYAHAFYPGS
                     GLEVLRGFISTNCGSSFSPLFELSGSNFATAPTITAVFLPTAAQWRRHTVDLAAYVGE
                     NVLIRFELENRYGNMTYLDDIRVAGQTTSVAESAPEPVSVWPVPTSDHLMVSGSRDFR
                     ISSLRLFNGLGRQVSHYKAPDAAAPLRIERGALPSGWYVLEITGAGGQREMRKVAFQ"
     gene            7066..7278
                     /locus_tag="PK198_14705"
     CDS             7066..7278
                     /locus_tag="PK198_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40038.1"
                     /translation="MKKLMLCLTLLIAAQFNLLAATEIPKVVQDAFARLYPQINTMNW
                     EHRPEGMVATFVDEEKSFLQRRWPLD"
     gene            7229..7561
                     /locus_tag="PK198_14710"
     CDS             7229..7561
                     /locus_tag="PK198_14710"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40039.1"
                     /translation="MWTKKKAFFNADGHWIETRIRMATDQLPAPVQKFIQNNYRGASI
                     SFAGRVYDPNGVWYRVESELSDRVVLKNLNTDGTLMEERTIWFSTSLPLSKPANIQGI
                     TPKVVRGY"
     gene            complement(7681..>8441)
                     /locus_tag="PK198_14715"
     CDS             complement(7681..>8441)
                     /locus_tag="PK198_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40040.1"
                     /translation="TWYILLFTVLIVAAFVHFYRLSEAKQVLEVVSWNNEKQQSLRSG
                     PVWFGYDEQARLLKVKGVIDDTVKMQLLKLYPSDSSGYASYQSAIDLLAFKTQTSANK
                     PPYLLVLVLGGLAAMIGVQIRTIYHFLDHACYKRDFDAKIWWPWYVVRPWVGFLLGAA
                     LILLAEVNLFSVANAQYSQIFWIGFSILAGFSTPDVIRRLGKASEAIFGIDNNPRPTP
                     VPTTPAAPENDGATIPPATQPEPEAADGQDNANL"
BASE COUNT         2118 a         2230 c         2248 g         1813 t
ORIGIN      
        1 ccggcggttt ttttcggcct gctgctgggc gattggattg acagcctgct ggagaacgta
       61 gtggtggtag gcgtcatgct ggtgatgggc ggcattgttt ttttgatggt ggatcgctgg
      121 tttgaaaaag ccgaaacctc ccccgaccgg cctgtttcct acaaagaagc cctgcgcatc
      181 ggtctgttcc agtgcttagc catgattccc ggcgtatcgc gttcggcggc taccatcatc
      241 ggcggcttgg cgcagggact gaaccgcaaa acagcggcag agttctcgtt tttcctcgcg
      301 gtgccgacga tgtttgcggc tacggccaaa aaaatgctgg actactatca ggacggtatt
      361 tcgctgagca gcggcgagtg gcaattgctg gcggtgggca atgtggtagc tttcatcgta
      421 gccatgattg ccatcaaaac cttcatcacg tttttggcgc ggcacggctt caagtggttc
      481 ggttattacc gcatcatcgc cggggcggtc attttgctgt tgtactttac gggcaagctg
      541 gcctggggcg tataaatgga ttgacggcag cgtttttcta aaccctttca agccnnnnnn
      601 nnnnnnnaat cggccctttt acctgttcag gaatgaagcc ccttactttt tccctccaat
      661 cctttggcga atttaaatac ttcgcttagt ttgcgccgca aaacggatgt ctgatgaatt
      721 taaataccaa cggaaaagat tccgtcactt atgacgctat tgtcgtaggg acgggcatca
      781 gcggcggatg ggccgctaaa gaactgtgcg agaaaggttt aaaaaccctc gtactcgaac
      841 gcgggcgcat ggtcaaacat ggagagtacc ccacggccaa tatggacccc tgggatgacg
      901 agcaccgcga ctacgccacg caggaagaac tcaaggatta cgaaaagcaa aaccggacgg
      961 gctacaccat tcggcccagt tcgaagcact ggtttgtgaa cgaccgcgag aatccgtact
     1021 ccgaaaccaa gcgtttcgac tggatgcgcg gctaccacgt cggcgggcgt tccatcatgt
     1081 gggggcggca atcgtatcgc ctcagcgacc tcgactttga ggccaatgcc aaagacggca
     1141 tcgcggtgga ttggcccatt cgatacgccg acctcgcgcc ctggtacgac catgtggaga
     1201 gttttgcggg catcagcggg caggccgagg gtattccgca tctgcccgac ggcaaattcc
     1261 tgccgccaat ggagtacaac tgcctcgaaa agcaggtcaa agagcgcatt gagtccaagt
     1321 ttccgggccg caaaatgatc atgggccgta ccgctcacct cacgcaggcg cacaatgggc
     1381 gtggcaactg ccagttccgc aaccgctgta tccggggctg cccttacggc gcttatttca
     1441 gcagcaatgc ctccacgctg cccgctgccg aaaaaacggg caacatgacc ctgcggcctt
     1501 actccattgt acacagcatc attttcgacg aggctaaaaa caaggctgtc ggtgtgcgcg
     1561 tcgtggattc ggaaaccctc gaaacacacg agtattttgc caaagtcatc ttcctctgcg
     1621 catcggcatt gggcagcacc cagattctga tgcagtccac atcgcgccgt ttccccaatg
     1681 gattcggcaa cgacagcggc gtactgggcc actacctgat ggaccaccac ttccgcgnnn
     1741 nnnnnnnnnn nnnnnnccga tgattttcag gatcagtatt acaaaggccg ccgaccaaac
     1801 ggcatctata ttccgcgttt ccaaaacctg aacgatgcca ccaaacgcga ctacctgcgc
     1861 ggcttcggct atcagggcgg cgccagtcgg atggactgga ccgagagcat ccgcgaaatg
     1921 caggtgggcg tcggcaagga ttttaaagac agcctcgtaa ggcccggcca ttgggaaatg
     1981 ggcatcatgg gcttcggcga atgcctgcct taccacgaca acaaagtgac gctgaacaaa
     2041 gacgtactcg acaaatacgg attgcccacg ctgacgatgg atgccgagtt caaagaaaat
     2101 gaaatgaaaa tgcgcaagga catgatgttc tacgccgctg aaatgctgga agctgccgga
     2161 ctcaggaatg tggaaaccta cgacagccag tcatggccgg gcttggccat ccacgaaatg
     2221 ggtacggcgc gcatgggccg cgatccgaaa acatcggtgc tcaacggctg gaatcaggta
     2281 cacgccgcac ccaatgtatt cgttaccgac ggcgcctgca tgacctctgc ggcctgccag
     2341 aatccgtcgc tgacctacat ggccctcacc gcccgcgccg ccgattatgc tgttcaggaa
     2401 ttaaaaaaac tcaatctgta attcgtcatg gatagaagag aagccatcaa aaagaccatg
     2461 ctgatcaccg ggtacgccct caccgcctcc actgtgcagg ccgtactgac gagctgcgaa
     2521 accgagcgcg aagtgaattg gacacccaaa tacttcagcg ccgaacaggc cgccctcatt
     2581 acgaacatgg cggaaaccat cttgccgcgt accaaaacgc ccggcgccat agacgtgggc
     2641 gtgcccgctt ttatggaacg tatggtgaaa gatacttacc tgccggaaga tcaggagcaa
     2701 ttcgctgccg gtttggccga tgtggaggcc cgcgcacaaa aagcgcacgg caagcctttt
     2761 gccgcctgca aaataaagca acaggccaac atcctcgaag cgctggatgc ggaagcttct
     2821 gctgccggga tagcgcgccg ggatgccgtt cgcaacaatc cggagttggg cgcagagccg
     2881 tatttcaact tcttcctcaa cttcaaatcg ctggccttgc tggggtattt cacctcttct
     2941 tttgtaggaa ccaaagtgct gagttacgat cccattccgg gcgcgtatct gggctgtatt
     3001 cctgccaaag aagtgggcaa tgcctggtcc ttataagcag ataacgccgg aaggatatct
     3061 gtctttccgg cgtttatcat aaaattcaaa acttcgttat tgatatgaaa aaatcaagcc
     3121 ttatcgttgc cttctcgctg gcaatttcaa tccttttcgg ccaaatcgaa gccgaccagt
     3181 tgtaccaaaa caacgagttt gcaaaggcca tgccgctgtt caaacaactg tcggcggaca
     3241 accccggcaa ctacgcccat tttgaaaaat atgtactgtg cctcatgcag ttgcaggaaa
     3301 tggaccaggc tgaaaccgcc atgaaagagc gtttgaaaac cgacagtacc aaccccgaat
     3361 tgtacttcct gtacggctcg tttttgtcga ggcagggccg cagctccgaa gctgattcca
     3421 taaggcaaca gcgccgcaaa ttgctggatg aaggttacgg cgccaagcaa tgggccgcag
     3481 cgccgccggc aagttctccg tcgccacctg tgaaaagaga cgaaattgaa cctataaaag
     3541 ctgaagaggc cgacgaaatg cctttgtggt tgcaccccga ttgcgaacaa gtgaccgacg
     3601 tcaaggagcg aaagttttgc agcgacaaca aaatgctgga gcatatttac aagaacgtcc
     3661 tttatcccgt tgaagccaga gagaatggcg ctcagggcac ggccgtgatc acttttgtca
     3721 ttgaaaaaga cggcagtatg acgagcctcc gcatctttcg cgaccccggg tacggtatag
     3781 gggaagcggc tctaagcgca gtgcagcgaa tgcaatcaag tggtatcgcc tggaagccgg
     3841 gcaagcaaaa aggcatgccg gtgcgggtgc agtttaatat gccggtacga ttcaaactga
     3901 cggagggcgg ccaaaaatag tacctgtctc cgggcaaccg ccaacggcgg ttgaatacga
     3961 atagccccgc ttattcaagc ggcattcgtt ggttgaaatc gctacatttt gcatttgtat
     4021 atccaataga ataaaatcac ccaatatttt ttttaaaact tatcttttaa caatcagcac
     4081 aacttgtccc gataccgcag gaatcgggat cagcacaact tgttccgatg acgtaggaat
     4141 cggggtatta aaataatctt tacttttgct ccattcactc aaaactaatt gcttcaacat
     4201 gaaaaaatca acctttttcg ccatctccgc gctttttgcc ggcagccttt ttgcacaaac
     4261 aggaaccatg ccggtagtta tggaggaaga tatcatcaac gccaaagatg ccgacgaaat
     4321 gccgctgtat ttacattcgg agtgcaataa gcaaactgaa tacgaagccc gaaagcgctg
     4381 cgccgacaaa aaaatgctgg agtttgttta cgaaaacact caataccccg aaaacgccag
     4441 aacgcagcgc aaagaaggca cggtggtcgt cacttttgtg gtagaaaaag acggcaccat
     4501 gacagacccg cgcattgctc gcgatcccgg ctatgacatg agcgaagaag cgctgagagt
     4561 cataaaacag atgcaggcaa aaggcgtagt ctggaagccg ggcacgaaag gtggcgagct
     4621 ggtgcgagtg cagttttata tccccatccg ctataagctg aaataaccct actgaaatgc
     4681 caccttgcgc atttcccgtt ggccgccggc gccggtaatt tccaaaacat accagccgga
     4741 aggcaatgct ccgcgctcta tccgcagagg agcggccgcg tctggagcct tgtagtgcga
     4801 cacctgccgc cccaatccgt tgaacagccg cagcgatgaa atgcgaaaat cccggctgcc
     4861 gctcaccatc aggtggtctg aggtaggaac gggccacacc gagacaggtt ccggcgcaga
     4921 ctcggcaaca gatgtggtct gccccgccac ccggatgtcg tccaagtagg tcatgttgcc
     4981 atatcggttt tccaattcaa accggataag tacgttttcg ccgacgtaag cagccagatc
     5041 aaccgtgtgc cggcgccatt gtgccgcagt gggtaaaaac acagcagtga tggtaggcgc
     5101 agtggcaaag ttgctgcccg acaactcaaa taatggactg aaagacgaac cgcaattggt
     5161 agaaataaag ccccgcaata cctccagccc cgaacccgga taaaacgcat gggcgtagga
     5221 gaaactgagt atagagcctg tatttgcttg aatcagaggc agttcaaagg cgtatctgtt
     5281 gtcttcattt ccatcgtaat aattgatgcg agccagagag tgtcccggtg tctggtggcc
     5341 ggcaaagcgc gtcgttcgcc agtctaagat attgccggtt tgcaatgaaa ccaagctgcc
     5401 gggaaactcc ccgcccgatt caaaatcttc ataaaaatcc gtttgggccg cctcgttatt
     5461 ttcaaaacgg tagtaagcac tgcgctcatt gttggcaggc atgtcatccg gcgtgtccaa
     5521 aatggccttt acctgcaagc ggtacttttg cccgtcggct ggccccagat tgaaagcgag
     5581 cggcagcaag cgttggtctc ctggaggaag gccctcgccc agcgacaccg ccagcgtatc
     5641 ctgccattcg ccgtctatac ccagtgccat ttgcaactga tgcagcgtgg ccgtgccttt
     5701 gttttgaacc gtcacaagcg gcgtttgcgg caggcaactc gttcggttgg gtgcgagatt
     5761 ttggatggaa acatcgtgcg actgggtcgg aaggcggttg agcgcttcgg caaacaattc
     5821 caagccttcc gtcgtcaccg aagcgccgta ttggttggca ataatacaca gcgaaatgcc
     5881 tgtggacgga tcgtgcagca tgtacgagcg gaaaccccat attccgacat ggtaatacac
     5941 cgagcggcca tagatgttgc gcagccggat gccgagcgcg tatgccatgt acccatcgtt
     6001 attggtcaaa tcgtggcggc tcccggcgct cagccaggta tggccgaaca gcgcatcgta
     6061 ccagcgcacc atatcgtagg ctgttgacca gacagacccc gctgtagaag taaaagtgag
     6121 aaaaccccgc gatccgccgg gcgtgagcgt ggtggcagtg ccggccgtat tccagaggtt
     6181 actgtacggc gcagcgaata cattatcgga gggcggatag gccattgcca gtccaagcgg
     6241 cgtaaaaatg cggctgtgca gaagctgatg aatgggttgc ccggcagccg tttccgcgat
     6301 gcgccccagc aggaggtagt tggtattgct gtagctgtag ccggcatcgg gtgcggaaac
     6361 cggagcgccc acagtggcca agagttcttc gtaggtccag acgtggttgg ggttggcgct
     6421 gtacgcggcc tctgtggcgc tcaaccactg gttgataaaa tcgaacaggc ccgaagtgtg
     6481 ctccagcaat tggcggatgg tgatttgagg gttgatctgc ggatgaggcg gcaggtagtc
     6541 gctgattgga tcgtctaaat gcaataagcc ctcgtcctgc aatttaagga tcagtcctgc
     6601 aataatggct ttggaattgc tggccatacc gaaccttata ttggtcgtca caggagtatt
     6661 cacggcagcg cggccccagg cgcccgtcca gatgccttct cccggaacca ataccgccgc
     6721 agtgaggcct ccggcgaaat tgccgtttgt acccagttgg ttgagcttgg tttgcaattg
     6781 ctgggcatac aatggatcaa aaccggacgt ggtggattgc gcaatggcat cagaccacca
     6841 tatagcgatc aagatacagg ccagtcctaa acgaaacgag aacaccatag ggatagactt
     6901 tgacggttga taaataccaa caaaggtaag cgggcttccg ccaagatgcg tggtatgttt
     6961 gaatcgtatt ccaaaaataa aagcagaaac agcaaacact taatatattt acttgtatat
     7021 gtaatagtaa tactaataat tacatcattc aaatcacaaa aaaccatgaa aaaactgatg
     7081 ttatgtttga cgttgttgat cgctgcccaa ttcaatttgt tggccgccac agaaatcccc
     7141 aaggtagttc aagatgcttt tgcccgcttg taccctcaga tcaacaccat gaactgggag
     7201 caccgcccgg aaggaatggt cgctacattt gtggacgaag aaaaaagctt tcttcaacgc
     7261 agatggccac tggattgaaa cgcgcatccg catggcgacg gatcaattgc ccgcgccggt
     7321 acaaaaattc atccaaaaca attatcgggg tgcgtccata tctttcgccg gcagggtgta
     7381 cgatccgaat ggggtgtggt atcgggtaga gtccgagttg tcggaccggg ttgtattaaa
     7441 aaatctgaac acagacggca cactgatgga agagcggacg atctggttca gtacctccct
     7501 gcccctgtcg aaaccggcca acatacaggg cataacgccg aaggtggtga gggggtattg
     7561 agacgctgcc ttaattccat gaacattttt ggaggctcga tgagaaccgg cgtatggttt
     7621 cagtcgggcc tccgcccttt caggaaaatt cttgctgcgg acgctacacc ctataccatc
     7681 tcagagattg gcattgtcct gtccatctgc cgcctctggt tcgggttgag tcgcaggcgg
     7741 aattgttgct ccgtcgttct ccggcgcggc aggggtggtt ggaaccggcg taggccgcgg
     7801 attgttgtca atgccgaaaa tcgcctctga ggctttgccc aggcgccgaa tcacatcagg
     7861 cgtgctgaac cctgccaaaa tagagaaccc gatccaaaaa atctgggaat actgcgcatt
     7921 ggccaccgaa aacagattga cttctgccag taagatcaaa gcagccccca gtaggaaacc
     7981 tacccaggga cgcacaacgt accaaggcca ccagattttg gcgtcgaaat ctcgtttgta
     8041 acaagcatga tccagaaagt gataaatggt tcggatttgc accccgatca tggccgccag
     8101 cccgcccaat accagcacga gcaaataagg aggcttgttg gccgaggtct gtgttttgaa
     8161 agccagcaag tcaatggcgc tctggtagga ggcataaccg gagctgtcgg aaggataaag
     8221 tttcaacaac tgcattttta cggtatcatc aataacgccc tttactttca gcaatcttgc
     8281 ctgctcgtca tagccgaacc agacggggcc ggagcgcagt gattgttgct tttcgttgtt
     8341 ccaggaaact acctccagta cctgcttggc ttccgatagc ctgtaaaaat gtacaaaagc
     8401 agcaacgatc aatactgtaa acagcaaaat gtaccaagtc a
//
LOCUS       DAORLF010000188         8433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4557_length_8433_cov_1.958463, whole genome shotgun sequence.
ACCESSION   DAORLF010000188 DAORLF010000000
VERSION     DAORLF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_8433_cov_1.958463"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..>1387)
                     /locus_tag="PK198_14720"
     CDS             complement(127..>1387)
                     /locus_tag="PK198_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF40041.1"
                     /translation="SHKAQILNEAVEGPITPSVPATFLQKHPNVKVYADIAACEELTR
                     IKTPWLVGMCNWNEDLVCKAVVWLSLKTGKPILKLTDEDYSENQLSDLTIEYDDAYNI
                     NIRIFNRLQHTITGWPGGKPNADDSHRPERAKPAKKRVIIFSPHPDDDVISMGGTFLR
                     LVEQGHDVHVAYQTSGNIAVHDHDAMRFMEFVQEYAAATGIANDTLSTVYQKVMDSLA
                     QKQTKENDIDEVRKIKGFIRRGEARAGARFCGLPDDHIHFLDMPFYETGRTRKKPIGE
                     DDVRIIMELMEKVKPHQIYAAGDLADPHGTHRVCLDAIFGAFRQLQERAWMKDCWLWL
                     YRGAWHEWSVDEIEMAVPISPKQLERKRKAIFMHQSQKDKPPFPGSDDREFWQRAEQR
                     NRETARLYRSLGLAEYEAMEAFRRWKF"
     assembly_gap    1388..1564
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1565..2065)
                     /locus_tag="PK198_14725"
     CDS             complement(<1565..2065)
                     /locus_tag="PK198_14725"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRF40042.1"
                     /translation="MHNATATLKYQLKSLEKIPVKIWDDPKAGSLHLARSIALAIRQK
                     QQDGEKIVLGLATGSSPIQVYAELVRLHKEEGLSFRNVVSFNLDEYSPMQPEAQQSYV
                     HFMKEYLFDHVDIDPKNIHIPDGAVPMDDVEAYCERYEKMIDDHGGIDIQLLGIGRTG
                     HIGFNEP"
     gene            2337..3149
                     /locus_tag="PK198_14730"
     CDS             2337..3149
                     /locus_tag="PK198_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF40043.1"
                     /translation="MKYLHHHDRTIAYSTQGKGPAIVLLHGFCEDSTIWSDFQSALAD
                     AGFQVVCIDLPGFGGSEPIPHASIEQYAEAVEAVVGTLELSRFVLTGHSMGGYTALAF
                     AEKHPERLAGLGLFHSHPYADNESKKEARRKNIQFIENHGHIMFVKQLMPMLFAPAFA
                     ASNSYLVGKLVHAASMYPPVGIIGGLEAMIARPDRSHILKNSSCPVLFIVGEEDQAIP
                     ADQSLSQTVLPPTAAVHILEKTGHLGMLEAPKKTGRMLREFAGFCFHHASVQ"
     assembly_gap    3302..3380
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3381..3838
                     /locus_tag="PK198_14735"
     CDS             <3381..3838
                     /locus_tag="PK198_14735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRF40044.1"
                     /translation="VVAVWEEAGKFLVLIAYPFSRPFFNEPIDGIVYAVMIGMGFATL
                     ENIFYASQFGLGTTIIRAFTAVPAHALFSVIIGYFAGLAWLHPERKARLLLQGLGIAV
                     AFHAVYDFLVLQRYYDWLMILAVSGLWISIFFAQDLIRRHQDNSPFKPE"
     gene            3919..>4186
                     /locus_tag="PK198_14740"
     CDS             3919..>4186
                     /locus_tag="PK198_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HRF40045.1"
                     /translation="MESTIAPSPVMLYTEETPNPESLKFVTNKMLYRGTADFREEALA
                     REWSPIASALFDMPFVKGVYVTNNFVTVTKEMNYDWADIMLKIKE"
     assembly_gap    4187..4305
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4306..4539
                     /locus_tag="PK198_14745"
     CDS             <4306..4539
                     /locus_tag="PK198_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRF40046.1"
                     /translation="AELVRRIRELIDTYVKPAVEMDGGNIEFKSFHNGIVTVILQGSC
                     SGCPSSTVTLKAGIEGMLTRMIPEVKEVVSEMG"
     gene            4664..5326
                     /locus_tag="PK198_14750"
     CDS             4664..5326
                     /locus_tag="PK198_14750"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRF40047.1"
                     /translation="MLSKNEEDYLKALFHLIASQENDRAGTNQLAQQLDVKPASANSM
                     LKKLKTKGLVDYKKYGKLSLTSEGRVAALLLIRKHRLWETFLHQKLGFSWDEVHEVAE
                     QLEHIRSEKLIEMLDRFLGFPESDPHGDPIPDAQGNMNIRTKKTLAEVQPGESCRIAG
                     VKDTSAAFLQYLTQLGLRLDSQIKVLEIIDFDHSMRVALQDGREIHISEKFSQYVFVQ
                     QA"
     gene            complement(5328..6287)
                     /locus_tag="PK198_14755"
     CDS             complement(5328..6287)
                     /locus_tag="PK198_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40048.1"
                     /translation="MLAASIALVSQTYNIMGTTEDFLITWLLPSVILLYLAPSSLCAI
                     IYYIGIANWAVEMRGSESVWYWALLAAGVPHFVWAWRKNERLRYVLTGWALVLTFPFG
                     WFGTIEPEILLYGFWGTALVLGLMYLLESFPSAETHVSPAFQPLRTFSVIAVFVFLMT
                     LTYWDDYDPVVIEQLWKGGGYAAWAVRVNVAVWVAILAGYAWQFARQARQLPLQGVLP
                     AVFPLFLLLLMLIGPAVGDGLPRFLANVFLLLWGVSYLYSGIQHSRMGLVNLGMLIVL
                     MLIAFRFFDTEWSYLAKGIAFVLLGVAFLMVNWGLSKRLKQAG"
     gene            complement(6293..6634)
                     /locus_tag="PK198_14760"
     CDS             complement(6293..6634)
                     /locus_tag="PK198_14760"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRF40049.1"
                     /translation="MDTKRHLSWLYEQLPELVHKGLISPEQKDAIQSHYGPAHSGPAY
                     NWAFIVISIIGALLVGGGIILIFAYNWDELSRTWRTVLSIAPLVVGLIIYSYMLFRQR
                     HSIAWVEGSAT"
     gene            complement(6640..7494)
                     /locus_tag="PK198_14765"
     CDS             complement(6640..7494)
                     /locus_tag="PK198_14765"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRF40050.1"
                     /translation="METTVQAQSFVAAAYRNLAETGEKYHPVYVSNPPFPSIYFDDFF
                     DPDFLRDVLAEFPDLAQKKSNIYYNNPNENKHAGKGESDMGPNTRALVHYLNSEPFLE
                     FLQQLTGIEETLLPDPYLEGGGFHEIKPGGFLKLHVDFHKHKRSHLDRRLNLLVYLNE
                     DWDESYGGHFELWERDMSRCVTRIAPLFNRVALFSTTGDSWHGHPDPLTCPEGRSRKS
                     LALYYYTNGRPKHELKASEQQRITTTFAERKGMDSPAMRRYNTMVNLANRILPAGVVR
                     FIKKFRNT"
     assembly_gap    7964..8214
                     /estimated_length=251
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8215..>8433
                     /locus_tag="PK198_14770"
     CDS             <8215..>8433
                     /locus_tag="PK198_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40051.1"
                     /translation="PVTASAAFIETVNTDRIKDGIRVFDDPADLYQTITAATGRPYTE
                     LKAPETPHAAKTIMMPAITNISPLSVAAG"
BASE COUNT         1833 a         2084 c         2045 g         1845 t
ORIGIN      
        1 agcagatgag tcgtctgaac gagcagaaaa acgtagccgc agtggtgctg cggcgaggct
       61 tttgtgtgaa gtgcaggcag ctcagatgcc gttgaagatg tgtaggttat cggacccgtt
      121 cctcacctaa aacttccacc tcctgaacgc ctccatcgcc tcgtactcgg ctaggcccag
      181 cgagcgatac aggcgggccg tttctcggtt gcgttgctcg gcgcgttgcc aaaactcgcg
      241 gtcatcgctg ccgggaaatg gcggtttgtc cttttggctc tggtgcatga agatggcctt
      301 gcgcttgcgt tccaattgtt tgggactgat gggcacggcc atttcaattt cgtcaacact
      361 ccactcatgc caggccccgc gatacagcca cagccagcaa tccttcatcc aggcgcgctc
      421 ctgcaattgc cggaaggcgc caaaaatagc atccagacaa acccggtgcg taccgtgcgg
      481 atcggccaga tcgccggcgg catatatttg gtggggtttc actttttcca tcagttccat
      541 gatgatgcgc acgtcgtctt caccgatggg ctttttgcgg gtgcgtccgg tttcgtaaaa
      601 cggcatgtcc aaaaagtgaa tgtggtcgtc gggcaggccg cagaagcgtg ccccggcgcg
      661 agcttctcct ctgcgtataa accccttgat cttgcgcacc tcgtctatgt cgttttcttt
      721 ggtttgtttc tgcgccagcg agtccatcac cttttggtaa acggtagaga gcgtatcatt
      781 ggcaatacct gtggccgccg catactcctg cacaaactcc atgaaacgca tggcgtcgtg
      841 gtcatgcacg gcgatgttgc cggaggtttg gtaagccaca tgcacatcgt ggccctgctc
      901 tactaagcgc aggaaggtgc cgcccataga gatgacgtcg tcgtcggggt gcggactgaa
      961 gatgatgacg cgctttttgg cgggtttggc gcgttccggg cggtggctgt catcggcatt
     1021 gggtttgccg ccgggccagc cggtgatggt atgttgtaag cggttgaaaa tgcggatgtt
     1081 gatgttgtat gcatcatcgt attcgatggt caggtcgctg agttggttct cgctgtagtc
     1141 ttcatcggtg agtttgagga tgggcttgcc ggttttgagc gaaagccaga cgacggcctt
     1201 gcacaccaag tcttcgttcc agttgcacat gcctaccagc cagggcgttt tgatgcgcgt
     1261 gagttcttcg caggcggcta tgtcggcata cacttttaca ttggggtgtt tttgcaaaaa
     1321 agtagccggc acggaaggcg ttatggggcc ttcgacggct tcattcagaa tttgcgcttt
     1381 gtggctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnngggttc gttgaaacca atgtgcccgg tgcgcccgat gccgaggagt tgtatgtcaa
     1621 tgccgccgtg atcgtcaatc attttttcgt agcgttcgca gtaagcctcc acatcatcca
     1681 tggggacggc accgtcggga atatggatgt ttttggggtc aatgtccaca tgatcgaaga
     1741 ggtattcctt cataaaatgc acatagctct gctgggcttc gggctgcatg ggggagtact
     1801 cgtcaaggtt gaacgacacc acattgcgaa agctgaggcc ctcttccttg tgcaggcgca
     1861 ccaattcggc atacacctgt atgggcgacg atccggtggc caggccaagc actatttttt
     1921 cgccgtcctg ctgtttttga cgaatggcca atgcgatgga gcgggccaga tgcagagagc
     1981 cggctttggg gtcatcccag attttgacgg gtattttttc taaggatttt agttggtatt
     2041 tgagcgtggc ggttgcgttg tgcatgatcg gcaacggttt ggtgaatgtt caaatttcgc
     2101 tgcaaaatta gaagaaaaac ggctctgtgt acgcacacag aacgggtgtc ttttcagcat
     2161 tttgatccaa aggttcctta accggcaggc aatgatggcg caggggcgtc tgaaacctcg
     2221 aaaatcctga tgcactattc atcgggcttg ctacctttgc agtcagtagg cgcccggttt
     2281 catcttcagg ttatgaactt ttgaaagcgc gcctgcccca cataaccaat tacttgatga
     2341 aatacctaca ccaccacgac cgcaccatcg cctactccac acagggaaaa ggcccggcca
     2401 tcgtattgtt acatggtttc tgcgaagaca gcaccatttg gagcgacttc cagtcggccc
     2461 tcgccgatgc cggttttcag gtggtttgta tagacctgcc gggctttggc ggctccgaac
     2521 caatacccca tgcctccata gaacaatacg cggaagcggt ggaagctgtg gtgggcacac
     2581 tcgaattgtc tcggttcgta ctcacggggc actctatggg aggctacact gcgctggcat
     2641 ttgcagagaa acacccggag cgtttagccg ggctgggcct gtttcactcg cacccttatg
     2701 ccgacaatga gagtaaaaag gaggcgcgcc gaaagaacat tcaattcatt gaaaatcatg
     2761 ggcatatcat gtttgtcaag cagctcatgc ccatgctctt tgcccccgct tttgctgcca
     2821 gtaactccta cctcgtaggc aaattggtac acgcggcctc catgtatcct ccggtcggca
     2881 tcatcggagg cctcgaagcc atgatcgccc ggccggatcg aagccatatt ctcaaaaact
     2941 cctcctgccc ggtgcttttc atcgtaggcg aagaagatca ggccatcccc gccgatcaaa
     3001 gcctgtctca aaccgtactg ccgccgactg ccgccgtcca catccttgaa aaaacaggcc
     3061 acttgggtat gttggaggcg cctaaaaaga ccgggcgcat gttgcgggag tttgcaggat
     3121 tttgtttcca ccatgcctcc gtacagtaag cataatgacg ccgctactgc tcctactcgc
     3181 cattttgcca ggcttgctca tttcctggta tatctaccgg atggaccggc acgagcggga
     3241 gcatcgcgtg catctcattg cctgtttcgg gctgggtatg ttgctcacca ttcctgccac
     3301 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn tcgtggtggc cgtttgggaa gaggcgggca aatttttggt
     3421 actcattgct tacccatttt cgaggccctt tttcaatgag cctatagatg ggatcgttta
     3481 tgcagtgatg atcggtatgg ggttcgctac cctcgaaaac atcttctacg cctctcaatt
     3541 cggcctcggc accaccatca tccgcgcctt cacggcggtg cctgctcatg cgctgttttc
     3601 ggtcatcatc ggctacttcg ccggcttggc ctggctgcat cccgaacgca aagcccgcct
     3661 cttgttgcag ggcctcggca ttgctgtggc gtttcatgcg gtgtatgact ttctcgtgct
     3721 ccagcgctat tacgattggt tgatgatact tgccgtgtcc ggcctgtgga tcagcatttt
     3781 tttcgcgcag gacctcattc gcaggcatca ggacaactcg ccgtttaagc cggaatgaga
     3841 aatttgtatg aaaaccaaac ccgaagccga aattcgcgtt atgtaagcag atcaaatcta
     3901 aataaacgaa acataccgat ggaatcaacc atagcaccaa gtccggtcat gctctacaca
     3961 gaggaaacgc ccaacccgga atcgctcaaa tttgtgacca acaaaatgct ctaccggggc
     4021 actgccgact tcagagagga agccctggcc cgcgagtggt cgccgattgc atctgccctg
     4081 ttcgacatgc ccttcgtcaa aggcgtgtat gtgaccaata acttcgtcac ggtgaccaag
     4141 gaaatgaact acgactgggc cgacatcatg ctcaaaatca aggagtnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccga attggtgcgc
     4321 cgcatccgcg aactgattga cacttacgtg aagcctgccg tagaaatgga cggcggcaat
     4381 attgaattca aatctttcca caacggcatc gtcaccgtta ttctgcaagg ctcctgtagt
     4441 ggctgccctt ccagtacggt cacgctcaaa gccggcatcg agggcatgct cactcgcatg
     4501 atccccgaag tgaaagaagt cgtttccgaa atgggttaat ttgcaataaa cgattgaaca
     4561 cccatccagg ccgtctcgat ttttcggggc ggcctttgtt ttttatgaat caaccattcg
     4621 gccatgcggg ccgtttactg aattgatctt gcaccttaca cgcatgttga gcaaaaacga
     4681 agaagattac ctcaaggcac ttttccatct catcgcttct caggaaaacg acagggccgg
     4741 caccaaccaa ttggcccagc aattggatgt gaagcctgca tcggccaata gtatgctcaa
     4801 aaaattgaaa accaagggct tggtggatta taaaaaatac ggcaaactct cgctcacttc
     4861 agagggccga gtcgcggccc tgctattgat tcgcaagcac cgcctttggg aaacttttct
     4921 gcatcaaaaa cttggcttct cttgggatga agtacacgaa gtggccgaac aattggagca
     4981 tattcgctcg gaaaaactca tcgaaatgct ggatcgcttc ttgggtttcc ccgaatccga
     5041 cccgcatggc gaccccattc ccgacgcaca gggcaacatg aacatccgca ccaaaaaaac
     5101 actggcggaa gtccagcccg gcgaatcctg ccgcattgcc ggggtgaagg atacttcggc
     5161 cgcatttctg caatatctca cccagttggg gcttcggctt gattcccaaa tcaaagtatt
     5221 ggaaatcatt gattttgacc actctatgcg cgtagccctg caagatgggc gcgaaatcca
     5281 catttctgaa aagttttcgc agtacgtttt tgtgcagcaa gcctgattca gccggcctgc
     5341 ttcaatcgct tagacagacc ccagttgacc atcagaaaag ccacgcccag cagcacgaat
     5401 gcgatgcctt tggccaaata actccattca gtatcgaaaa accggaaggc aatgagcatc
     5461 aaaacgatga gcataccaag attgaccaag cccatgcgac tgtgctgaat gccggaatac
     5521 aaatacgaca ccccccaaag cagcaaaaac acattggcca aaaagcgagg cagtccgtcg
     5581 ccgacagcag gcccgatcag catcaacagc aacaaaaaca gcgggaaaac cgccggcagt
     5641 acgccttgaa gcgggagttg tcgtgcctgc ctggcgaatt gccacgcata acccgccaat
     5701 attgccaccc aaactgccac attcacccgc accgcccagg ccgcataccc gccgcctttc
     5761 cagagttgtt cgattacaac ggggtcataa tcatcccaat aagtgagcgt cattaaaaaa
     5821 acaaagacag ctattacgga gaatgtcctc agcggttgga atgccggact gacatgtgtt
     5881 tcagccgatg ggaaagattc caacaaatac atcaagccca ataccagagc cgtgccccaa
     5941 aatccataca gcagaatttc cggttcaatg gtaccaaacc acccaaacgg aaaagtgagt
     6001 actaatgccc agcccgtcag tacatagcgg aggcgttcgt ttttgcgcca ggcccataca
     6061 aaatgcggaa caccggcagc cagcaaggcc cagtaccaca ccgattcgga accccgcatt
     6121 tccacggccc aattggctat gccgatgtaa taaatgatgg cgcacaaaga cgaaggcgcg
     6181 aggtacagta aaatgacgga gggcagcagc caggtaatca gaaagtcttc cgtagtgccc
     6241 atgatgttgt acgtctggct caccaacgca atactggcgg ccagcatgag catcaggtgg
     6301 ccgaaccctc tacccaggct atggagtgtc gctgccggaa tagcatatag ctgtagataa
     6361 tcagccccac aactaagggc gcaatgctga gcacggtgcg ccaggtgcgg gacagctcgt
     6421 cccagttgta agcaaaaatg agaatgatgc cgccgcccac cagcaaagcg ccgatgatgc
     6481 tgatgacgat gaaggcccag ttgtaggcgg ggccgctgtg tgccgggccg taatgcgact
     6541 ggatggcgtc tttttgttcg gggctgataa gccccttgtg aacgagttcc ggcagttgtt
     6601 cgtacagcca ggagaggtgg cgtttggtat ccataaagat caggtgtttc tgaatttttt
     6661 aataaaacgc accacgccgg cgggtaatat gcggttggcc agattgacca tggtgttgta
     6721 gcggcgcatg gccgggctgt ccatgccttt gcgttcggca aaagtagtgg taatgcgctg
     6781 ttgttcagag gctttcaact cgtgtttcgg gcgtccgttg gtgtagtaat aaagcgccag
     6841 cgactttcgg ctgcggcctt cggggcaagt cagcgggtcg gggtggccgt gccaggaatc
     6901 gccggtggta gagaagagcg ccactcggtt gaagagcggg gcaatgcgcg tgacgcagcg
     6961 gctcatgtcg cgttcccaga gttcaaaatg gccgccgtag ctctcatccc agtcttcgtt
     7021 gagatagacc agcaggttga ggcgacggtc caaatggctg cgcttgtgtt tgtgaaagtc
     7081 cacatgcagc ttgagaaatc cgccgggctt gatctcgtga aagcccccgc cttcgaggta
     7141 gggatcgggc agcagcgttt cttcaatgcc ggtgagttgt tgcaaaaatt cgagaaacgg
     7201 ctccgaatta aggtagtgaa ccagcgcgcg cgtattgggc cccatatccg actcgccctt
     7261 gccggcatgc ttgttttcat tgggattatt atagtaaata ttgctcttct tctgcgccag
     7321 atcagggaat tcagccagca catcccgcag gaaatcgggg tcgaaaaaat cgtcgaaata
     7381 aatgctggga aagggtggat tgctcacata aaccgggtga tacttttcgc cggtttccgc
     7441 cagattccga tatgcagcag ctacaaaaga ttgcgcttga acagtggttt ccatgtatcg
     7501 taagtcattg gtaaataatg ggcgaagtta gggtttgtgc aagaaagggg cgatgaaaaa
     7561 aatggatttt cgttttgccc cagatcagag tggatttttt ttaaaccaat ttcattttta
     7621 acaatatttc aaattacatt tgcacactcc tgttttgtat cccacacaag caagagcatc
     7681 aaacaaagta tcctgttttt taaacctgtc atttttagca tacaaacatt tgaacaacac
     7741 aagcatggct gaatagtgta cacggccgct cacccggcct gaaatattgc atttcatcat
     7801 ttcaacacta tatatcacat gactgtgtat cgaatcatca tcacactttt tgcgttcgcg
     7861 tccatctgga acggaggcgc cgctgctcaa tgcgcccaac tgaacctgcc attggaaacc
     7921 cgcatcggcc tcagcgaggt catcattgaa ggccgggtag tggnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccgt
     8221 taccgcctcc gccgctttta tcgaaaccgt caacaccgac cgcatcaaag acggcatccg
     8281 ggttttcgat gatcctgccg acttgtatca aaccattacg gcagctaccg ggcggcctta
     8341 cacagaactg aaggccccgg aaacgcccca tgccgccaaa accataatga tgccggccat
     8401 taccaatatc tcacctcttt cagttgcggc ggg
//
LOCUS       DAORLF010000189         8428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4558_length_8428_cov_2.056013, whole genome shotgun sequence.
ACCESSION   DAORLF010000189 DAORLF010000000
VERSION     DAORLF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_8428_cov_2.056013"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    169..183
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <184..414
                     /locus_tag="PK198_14775"
     CDS             <184..414
                     /locus_tag="PK198_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40052.1"
                     /translation="IHIQPPLAVAREAHERLINEVIAKLSRHYWGVGQVVRRAQCGAL
                     QPTSNTARLQKGKTWMCRNFRPTNNFKKNLAI"
     gene            453..1091
                     /locus_tag="PK198_14780"
     CDS             453..1091
                     /locus_tag="PK198_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40053.1"
                     /translation="MSQNKIQDDLAQIRSIMERSTKFLSFSALASVLAGVYALIGAAW
                     AWRIIYFSEEVLYDHLKYEPLSPETLPLIFVAAGVLLAAVGTALLLSYRKAKRAGQSL
                     WSKSARRVFYNFALPMLAGGIFILVLYSRGFYSLLAASTLIFYGLALVNAGNFTFSDI
                     RVLGVWEVLLGLLAAVFPGKGLLYWALGFGVLHIIYGVVLYRKYERNSTETR"
     gene            1088..>1339
                     /locus_tag="PK198_14785"
     CDS             1088..>1339
                     /locus_tag="PK198_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRF40054.1"
                     /translation="MKNYLQELNKAFESRARLGIMSSLMVNDSITFNALKELLDLTDG
                     NLAGHTRALEQAGYLRVEKSFIGKKPNTRFFITPAGRQAF"
     assembly_gap    1340..1349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1544..2878
                     /gene="creD"
                     /locus_tag="PK198_14790"
     CDS             1544..2878
                     /gene="creD"
                     /locus_tag="PK198_14790"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRF40055.1"
                     /translation="MENNENSPISKLGHWLRTSLLVKLLSIGFIVLILLIPQVMIQGI
                     ISERQNRQYAAVQEISQSWGGDQYVQGPVLSIPYKEWTEYENGKRSYTLRTMYFLPTE
                     LNIESKAEHELRGRGIFDAILYRSDVRLNGKFEALAFRELNVAPDNIQWEEARLSVGI
                     SSMTGIKNIIELDWNGQKRRMEPGTANSELLASGVSAPVAVAEDAGSYTFSIPLELNG
                     TESLYFEPVGRVTTASMQSSWPSPSFTGKTLPDKREIGDKGFSAFWRVLDLNRNYPQQ
                     WKNDAHTFGDSSFGVRLIRPVDEYLKNDRAAKYAILVIGLTFLIYFFFETLRKFNIHP
                     FQYLLIGLALTVFYLLLLSLSEQIGFNAAYGTAAAATIGLISFYSASVLKTKRLVLQL
                     AMLLSAIYGFIFVVLQLEDYALLAGSIGIFAALAAVMYYSRQVDWYNLRGDA"
     gene            2890..4299
                     /locus_tag="PK198_14795"
     CDS             2890..4299
                     /locus_tag="PK198_14795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF40056.1"
                     /translation="MKIDIIIAYLKRYEFGHEKDFVPPITGIHLAAITPKEFEVRVFH
                     QQVDRIDYDTDAEVIAVSFFSGFAPAAFQLARTFRQKGKIVIAGGPHVTYNVEESLLH
                     FDAVVTGEAESAWPTLLNDLKNGALKNVYRGQASDMQSLPTPRYDLLPNRFFIKKVIQ
                     ATRGCPFSCSFCTVPTLNPGFRKRPVADVVRDASYDKFRFWWQRKIVWFWDDNLTIDR
                     KYIFELLPELKKLNKWWLTQASLDIAKDDKLLGLMKDSGCIGVFIGIESFGVESLKDA
                     NKRQNKIDNYQKAVRKIRKKGIAVMAGFIAGFDSDTEASIIEMADRIQDIGIDVPFLS
                     IMTPFKGTPIYETLRLEGRILEDRGWQFYNGYNVAFRPRHITPEGLLNAHRTLWKRAF
                     SPAYSLWRIWRGLFSLRFGAMCMSLFMNGFYMYKRLRGNLPADMSRQSVTSGAQTKAY
                     LATTTRVRPTGRVPSRNTI"
     gene            complement(4236..5174)
                     /locus_tag="PK198_14800"
     CDS             complement(4236..5174)
                     /locus_tag="PK198_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40057.1"
                     /translation="MRHLTFTLALLLSAGALAAQTEFAPVGAVWHYHKYEFFQFPWVP
                     IKFYRIEATGDTLIQGRSCSILETSDPIDCTRRRHKEYVSQQGDTIWLYDQDAEAFQL
                     LYNFGAQPDDTWTIDLVQVLSEMPLTVTIRVDSISTVNIVGTSLRRYYASHKIDSPEW
                     EGEFYPFPFPFTELLGAENYLFYIWGFEIGPLCDTAYPWGLRCYYDPNFGSYETGIAP
                     FCNWIGVPVRETLSEPEGVRVFPNPVSDRLHIENLRGAALAFELTDLHGRTLRRGINA
                     DFRTEVSVAQLPAGVYQMVFREGTRPVGRTRVVVAK"
     gene            5334..5915
                     /locus_tag="PK198_14805"
     CDS             5334..5915
                     /locus_tag="PK198_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF40058.1"
                     /translation="MKRITVFCGSSLGTDELYRHTAFRLGQMLAERGIGLVYGGAKVG
                     LMGAVADGALSRNGEVIGVIPTFMRGKELRHEGLSELILTASMHERKARMDELSEGVI
                     ALPGGFGTLDELFEMLTWGQLGLHGKPIGLLDVAGYYAPLMQMVQNMTDAGFLKPENQ
                     AMLLVDSDMEGLLTQMQNYQAPEVPKWITREEV"
     gene            complement(6017..7207)
                     /locus_tag="PK198_14810"
     CDS             complement(6017..7207)
                     /locus_tag="PK198_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF40059.1"
                     /translation="MYPSTAITTHSTNKIAMSSSFLRILLSLLLLVAGFFGGIAWKNR
                     HNQPIASLDQPAESPGSNETRSGAVPPDQGLSDSELATIRLFENAAPSVCYITTTNVG
                     YDFFTRNATEIPAGTGSGFVWDRNGHIITNYHVIQNADKATVTLADRSTWPARLVGKA
                     PEKDLAVLKIDAPASTLRPIPVGASDNLRVGQSVYAIGNPFGLDQTLTTGIVSALGRE
                     IQSVAGVPIRDVIQSDAAINPGNSGGPLLNSSGRLIGVNTAIYSPSGASAGIGFSIPA
                     DVVNWVIPELIRFGRIMRPSMGMEFARQSFVQRLGLEGALVLDVMQGEAADRAGIRPT
                     VRDRTGSIRLGDLIVAINKDKISSYSDIVLALEKYKPGEVVQLGIVRDGKQMTTDLKL
                     DAAK"
     gene            complement(7447..>8428)
                     /locus_tag="PK198_14815"
     CDS             complement(7447..>8428)
                     /locus_tag="PK198_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRF40060.1"
                     /translation="ELNQEAVKLEVAPKIAVYTPDFNEKGDRIQPWDDAVTLVLTYAE
                     IPYDPIYDREVLNDVLAKYDWLHLHHEDFTGQYGRFYRSYSSTPWYRENVRRSEEMAR
                     SLGFNKVSQLKLAVAKRIKEYVGGGGFMFAMCSATDTYDIALAAEGVDICANIYDGDP
                     ADPAAQQKLDYSKTFAFRDFTLVKNPLEYEFSNIDRPQTTNIVPEQDYITLFDFSAKW
                     DPIPTMLTQCHTRTVKGFMGQSTAFKRSLIKPEVLILGENKPAAEVRYIHGTFGQGTW
                     TFYGGHDPEDYRHYVEDPDTDLNLHPNSPGYRLILNNILFPAAKKKERKT"
BASE COUNT         1857 a         2131 c         2491 g         1924 t
ORIGIN      
        1 ttcaatttga atagccccgc gtccgacgta aggaggaacg cggggtagcg gtcgcgatga
       61 ggacaacccc caacgggggt tgaatgtcca acgagcgggt gtgaataatg acattgaacc
      121 cctgtcaggg ttcttcctcc cgtgatgcgc ataccccgcg ttcacttcnn nnnnnnnnnn
      181 nnnattcaca ttcaaccgcc gttggcggtt gcccgcgagg cacacgagcg cctgataaac
      241 gaagttattg caaaattaag ccgtcactat tggggtgtcg ggcaagtcgt gaggagggcg
      301 caatgcgggg cattgcaacc gacgagcaac accgcccggc tgcaaaaggg caagacttgg
      361 atgtgcagga atttccggcc aaccaataac ttcaaaaaaa acttggccat ttgaaaaact
      421 tcattaactt tgcgtcataa agtacttttt atatgtctca aaataaaata caggacgatc
      481 tggcgcaaat ccgttccatc atggagcgct ccaccaaatt cctgtctttc agcgcattgg
      541 cctcggtgct ggccggagtg tacgccctga taggcgccgc ttgggcttgg cgcatcatct
      601 atttttctga ggaggttttg tacgatcatc tgaagtacga gcctttgagt cccgaaactt
      661 tgccgcttat tttcgttgcg gcgggcgtat tgctggcggc ggtaggaacg gccttgttgc
      721 tgagttatcg caaagctaaa cgggcaggac agagcctgtg gtcgaagtcg gcgcgcaggg
      781 tattttacaa tttcgcactg cccatgctgg cgggcggcat tttcattttg gtgctgtaca
      841 gccgtggttt ttacagcttg ctggcggcct ccacgctcat tttttacgga ttggcgctcg
      901 tcaatgcggg caatttcacc ttcagcgata tacgggtgct gggcgtgtgg gaggtgctgc
      961 tgggactgct ggcggcggta tttcccggca aggggctgct gtactgggcc ttggggttcg
     1021 gggtgctgca catcatttac ggcgtcgttt tgtatcggaa atacgagcgc aactcaactg
     1081 aaacgaggtg aaaaattatt tgcaggaact gaataaggct ttcgagagcc gggccaggtt
     1141 gggcattatg tcgtcgttga tggtcaatga cagcatcact ttcaatgccc tcaaggaact
     1201 gctcgacctc accgacggca acctcgccgg gcatacccgc gccctcgaac aggcgggcta
     1261 cctgcgcgtg gagaagtcgt tcatcggcaa aaagcccaat acccggttct tcatcacgcc
     1321 ggcagggcgg caggcttttn nnnnnnnnng agaagattat cagggaggtg taggcaggtg
     1381 ggcggtaggc ggtgggcagt actaagcgta gagtgggcag cttgtggctt gcggcttgca
     1441 gtcttttata aaataccaaa cagatgcaat tttttttcac gccgcacaat tttttttgcc
     1501 ttttcacttt aaattacaaa gtactttaaa attatttaaa aacatggaaa acaacgaaaa
     1561 ttcacccatc tccaaactgg ggcactggct ccgtacttct ctgttggtca agctcttgtc
     1621 catcggattc atcgtattga tcctgctcat tccgcaggtg atgatacagg gcattatctc
     1681 cgaaaggcag aaccggcagt acgccgctgt gcaggaaatc agccagtcgt ggggcggcga
     1741 ccagtatgtg caggggccgg tactcagcat tccgtacaaa gaatggaccg agtacgaaaa
     1801 cggcaagcgg agctacacgc tgcgcaccat gtacttcctg cccaccgaac tcaacattga
     1861 gagcaaagcc gagcacgaac tgcgcgggcg cggcattttc gatgcgattt tgtaccgctc
     1921 cgatgtgcgg ctgaacggca aattcgaggc gctcgctttt cgggaattga acgtagcccc
     1981 agacaatatt caatgggaag aagcccggct ctcggtgggc atttccagca tgaccggcat
     2041 caaaaacatc atcgaattgg actggaacgg ccagaaacgc cgcatggaac ccggcacggc
     2101 caattcagag ctgctggcat cgggcgtgag cgctccggtc gccgttgcag aggatgccgg
     2161 ctcctacact ttttctatcc cgctggaact caatggcaca gaatcgctct attttgaacc
     2221 cgtaggccgc gtcactaccg cctccatgca gtcgtcgtgg ccctcgccga gctttaccgg
     2281 caaaaccctg cccgacaaac gggagatcgg cgacaagggc ttcagcgctt tctggcgggt
     2341 actcgatttg aaccggaatt acccgcagca atggaaaaac gatgcgcata ccttcggcga
     2401 ttcctccttc ggcgtgcggc tcattcgccc ggtggacgag tacctcaaaa acgaccgcgc
     2461 ggccaagtat gccatcctcg tcatcggact tacctttctg atctacttct tttttgaaac
     2521 gctgcgcaag ttcaatattc accccttcca gtacctgctc atcgggctgg cgctcacggt
     2581 attttatctg ctgctgctgt ctttgtcgga gcaaatcgga ttcaatgcag cctacggaac
     2641 tgcggcggcg gccaccatcg gactcatcag cttttacagc gcttcggtac tgaaaaccaa
     2701 gcgattggta cttcagttgg ccatgctgct gagcgccatt tacggcttca tcttcgtggt
     2761 gttgcaattg gaagactacg cgctgctggc cggcagtatc ggcatattcg cggcgttggc
     2821 ggcggtgatg tactactcgc gccaggtgga ttggtacaat ttgcggggcg acgcgtaaag
     2881 aactgtttta tgaaaataga catcatcatc gcgtatttga aacggtatga gtttgggcac
     2941 gaaaaagact tcgtgccccc cattaccggc atccacttgg ccgccattac gcctaaagaa
     3001 ttcgaagtca gggttttcca tcagcaggtg gaccggattg actacgacac agatgcggag
     3061 gtcattgccg tttctttttt cagcggattt gcgccggctg cgttccaatt ggccagaaca
     3121 ttccggcaaa aaggcaaaat cgtcattgcc ggcggcccgc acgtgacgta caacgtagaa
     3181 gaatcgctgc tccatttcga cgcggtggtg accggagagg ccgaatctgc ctggcctacg
     3241 ctgctgaatg acctcaaaaa cggagcgctg aaaaacgtgt accgggggca ggcgagtgat
     3301 atgcagagct tgcctacgcc gaggtacgac ctgctgccca atcgtttttt tatcaaaaaa
     3361 gtcattcagg ccactcgcgg ttgtcctttc agttgctcgt tttgcaccgt gcccacgctc
     3421 aatccggggt tcagaaaacg gcccgttgcc gatgtggtca gagatgcgtc ttacgacaag
     3481 tttcgttttt ggtggcaacg aaagatcgtc tggttttggg acgacaacct gaccattgac
     3541 cggaagtata tttttgaact gctgccggag ttgaaaaaac tgaataaatg gtggctcacg
     3601 caggccagtt tagacattgc gaaagatgac aaattgctgg gtttgatgaa agattccggt
     3661 tgcatcggcg tgttcatcgg cattgaatct ttcggggtag aatcactgaa agatgccaat
     3721 aagcgccaaa ataagattga caattaccag aaggccgttc gcaaaatcag aaaaaaaggc
     3781 attgccgtga tggcggggtt cattgcgggc ttcgacagcg ataccgaagc gagcatcatc
     3841 gaaatggccg accggattca ggacatcggc attgacgtgc cctttctcag cattatgacg
     3901 cccttcaaag gtacgcccat ttatgaaacc ttgcgcctgg agggtcgcat tctggaagat
     3961 cggggctggc agttttacaa tggctacaac gtggcttttc gtccccggca catcacgccc
     4021 gagggcttgc tgaatgctca ccggaccttg tggaagcggg cattttcacc ggcctattcg
     4081 ctgtggcgca tttggcgagg gttgttctct ctgcggttcg gggcgatgtg tatgtccttg
     4141 tttatgaacg gcttctacat gtacaaacgc ctgcggggca atttaccggc agatatgagt
     4201 cggcaaagcg tcacatcggg cgctcagacc aaagcttact tggccaccac cacccgcgtg
     4261 cggcccaccg ggcgggttcc ctctcggaac accatctgat acacgcccgc cggcagttgc
     4321 gccaccgata cctctgtgcg aaagtctgcg ttgatgcccc ggcgcagcgt gcggccatgc
     4381 aggtccgtca gctcgaaggc aagcgccgcg ccgcgcaggt tttcgatgtg cagccggtca
     4441 gacacggggt ttgggaagac gcggacgcct tcgggttcgg acagggtttc gcggacgggc
     4501 acgcctatcc aattgcaaaa aggagcaatg ccggtttcat aagaaccgaa attggggtcg
     4561 taataacagc gaaggcccca cggataggct gtgtcacaaa gaggccctat ttcaaacccc
     4621 cagatataaa agagataatt ttctgcgcct aacagctctg taaacggaaa gggaaaagga
     4681 tagaattcgc cctcccattc cgggctgtct attttatgag atgcataata ccggcgcaat
     4741 gaagtaccca caatgttcac cgtactgatc gaatctaccc tgatggtgac cgtcagaggc
     4801 atttcactga gcacttgaac caagtcaatg gtccaggtat cgtccggctg cgctccaaaa
     4861 ttgtataata attgaaaggc ttcggcatcc tgatcgtaaa gccagatcgt atcgccctgt
     4921 tgagacacgt attctttgtg gcggcgacgc gtacaatcaa taggatcgga ggtttccaaa
     4981 atactgcacg aacgcccctg tatgagcgta tcgcccgtag cttctatgcg gtaaaacttg
     5041 atgggtaccc acgggaattg aaaaaattca tacttgtgat aatgccacac agcccccaca
     5101 ggcgcaaatt cagtttgggc ggccagggcg ccggcactca aaagcaatgc aagggtaaag
     5161 gtcagatgcc tcataatgat tgggttttaa tcaaaaacgg gatgtacaaa agtacgacat
     5221 aacccacaga gaaacaagcg ctcatacagg gtttgtcggt cggcttgcat tttgggtgga
     5281 aataacgctg ttcaaaacac cctatcttgc cgcccaaaat catcgccttc accatgaaaa
     5341 gaatcactgt tttttgcggc tcctccctcg gcacggatga gctgtaccgc cacacagctt
     5401 tccggctggg gcaaatgctg gcagagcgcg gcatcggcct cgtatatggc ggcgccaaag
     5461 tgggcctgat gggagccgtg gccgacggcg cactgagccg aaacggggag gtcatcggcg
     5521 tcattcccac ttttatgcgc ggcaaggaat tgcgccacga gggcctcagc gaactcatcc
     5581 tcaccgccag tatgcacgag cgcaaagccc gcatggacga actttccgaa ggggtcattg
     5641 cgctgcccgg cggcttcggc actttggacg aactgtttga aatgctcacc tgggggcagt
     5701 tgggcctgca tggcaaaccc attggtctgc tcgatgtggc gggctactat gcgccgctga
     5761 tgcaaatggt gcaaaacatg accgacgcgg gttttttgaa accggaaaat caggctatgt
     5821 tactggttga cagcgatatg gaagggctgc ttacgcaaat gcaaaactac caggcgccgg
     5881 aagtgcccaa gtggataacg agggaggagg tgtaggatcg gctgtttgcg tataagcatc
     5941 gctttgacct tcgcagaagg caaagccatc caaaacggac tggaaaatgc tgagaaccag
     6001 aaaagtagcg cccgctctac ttcgctgcat ccaatttcag gtcggtcgtc atttgtttgc
     6061 cgtcgcggac gatgccgagt tgtactacct cgccaggttt gtacttttcg agggccagca
     6121 caatgtcgct gtaagaactg attttgtctt tgttgatggc cacgatgagg tcgccgagac
     6181 ggatggaacc ggtgcggtcg cgcacggtgg ggcgtatgcc ggcgcggtcg gcggcttcgc
     6241 cctgcatgac gtcgaggact aaagccccct caagccccaa gcgctgcacg aaagactgcc
     6301 gggcaaactc catgcccata gagggccgca tgatgcgccc gaagcgaatg agttcgggaa
     6361 tgacccagtt gacgacgtcg gcagggatgg agaagccgat gcccgccgat gcgccggaag
     6421 ggctgtaaat ggcagtgttg acgccaatga gcctgccgga agagttgagc agcgggccgc
     6481 cggaattgcc gggattgatg gctgcgtcgg attgaatcac atcgcggatg ggtacgccgg
     6541 ctaccgactg aatctcgcgg cccagagcgc tgacgatgcc ggtagtgagc gtttggtcga
     6601 gaccgaatgg attgccgatg gcatacaccg actgcccgac gcggagatta tcggaagcgc
     6661 ctacggggat gggccggagg gtggaggcgg gtgcgtctat tttgagcacg gcgaggtctt
     6721 tttcgggggc cttgcccacg aggcgggcgg gccaggtgct gcggtcggcc agggtgacgg
     6781 tggccttatc ggcgttctgg atgacgtggt aattggtgat gatgtggccg ttgcgatccc
     6841 agacgaaacc agagccggtg ccggcgggaa tctcggtggc gttgcgggtg aagaagtcgt
     6901 agcccacgtt ggtagttgtg atgtaacata ccgagggcgc tgcgttttcg aagagacgga
     6961 tggtagccaa ctcgctgtcg ctcaggccct gatcgggtgg aacggcgccg ctgcgggtct
     7021 cgttgctgcc cggcgattcg gcgggctgat cgagggaggc gatgggctgg ttatggcggt
     7081 ttttccaggc gatgccgccg aagaatccgg caactaagag caggaggctg aggaggatgc
     7141 ggaggaagga ggatgacatg gcaatcttat tggttgaatg agtggtaatg gctgtggatg
     7201 gatacatggc gattgtgttt aaggttcaaa aagtttcggg tttcatccgc cgtagcctta
     7261 gcgaaggcgg gttcaaggtt caaagtttcg ggttggaacg gcttctggct gcaagccgcg
     7321 cactccacgc ttggcaccgc ctaccccgcg ccacggcgcg gctaccgggt tcaaggtttc
     7381 gggttccctc tacgcttggc accgcctacc gcacaccgca tactgatcta agccttgcgg
     7441 agtactttac gttttgcgtt cttttttctt ggctgcaggg aagagtatgt tgttgagaat
     7501 gaggcgataa cccggcgaat tgggatgcag attgaggtcg gtgtccgggt cttccacata
     7561 gtggcgatag tcttccgggt cgtggcctcc gtagaaagtc caggtgccct ggccgaaggt
     7621 gccgtggatg tagcgcactt cggcggcggg cttgttttcg ccgaggatga gcacttccgg
     7681 tttgatgagc gaccgtttga aggcggtgct ttggcccatg aaacccttga cggtgcgggt
     7741 gtggcactgg gtgagcatgg tgggaatggg gtcccatttg gccgaaaaat cgaacagcgt
     7801 gatgtagtcc tgttcgggta cgatgttggt ggtttgcggg cggtcaatat tggaaaactc
     7861 gtattccagc gggtttttta cgagggtaaa atcgcggaag gcgaaggttt tgctgtaatc
     7921 gagtttttgc tgggcggcgg gatcggccgg gtcgccgtcg taaatattgg cgcagatgtc
     7981 aacgccctcg gcggccaatg cgatgtcgta agtatctgtt gcagagcaca tagcgaacat
     8041 gaatccgcct ccgccgacgt attctttgat gcgtttggct acggcgagtt tgagttgcga
     8101 tactttgttg aagcccagac tgcgggccat ttcttcggag cggcgcacgt tttcgcgata
     8161 ccagggggtg gagctatagg agcggtaaaa gcgtccgtat tggccggtaa aatcttcgtg
     8221 gtgcaagtgc agccagtcgt atttggccag cacatcgttg agcacttcgc ggtcgtagat
     8281 gggatcgtag ggaatctcgg cgtaggtgag cacgagggtc acggcgtcgt cccagggttg
     8341 gatgcggtcg cctttttcgt taaaatcggg ggtgtaaacg gcgattttgg gagctacttc
     8401 gagcttgacg gcctcctgat tgagttcg
//
LOCUS       DAORLF010000190         8420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4566_length_8420_cov_2.013509, whole genome shotgun sequence.
ACCESSION   DAORLF010000190 DAORLF010000000
VERSION     DAORLF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_8420_cov_2.013509"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    243..370
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<371..>1786)
                     /locus_tag="PK198_14820"
     CDS             complement(<371..>1786)
                     /locus_tag="PK198_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40061.1"
                     /translation="GILQWRKPIDVRSQTYAKRIFPTADGNFLLLGRANDIGAGFKGI
                     FIRKIDPAGNLIWQKTYDTGFEERDYNMPVSGEFFSEPLGMVFMPDESIWMANPYQTF
                     AEEVALLHFSAEGVLLEQKIYGSAGWREFPYALYALPDGGWMICGNALSTSGNADING
                     FAMRVTADGLEVWRRYYGESTSRERIFTGAALPDGQFLLAGLSNTPAGGGSAASSAWL
                     LRAEADGNARLWRVDGKVVIDLNGNCVADADEPPAVGWFVRAENHSAHILITDAEGNF
                     TFHTEDAVTEFTVRAPDESAWSLCNNVQTIESNSAHPSANLTFTAYQNDGGCPLTEVG
                     ITQPDLVRCDTSRFFVTITNRGVGESGDLLLQVRLHPALQVVSASAPFIQNGGSIEFA
                     VPPMQGLQSQTFEIWAQLDCNARLGASHPVVAEIVPAECAPGWSGPRYIVEGRCDGDN
                     VRFDLFNRGGGGAGAETFYRV"
     assembly_gap    1787..1837
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1838..2269)
                     /locus_tag="PK198_14825"
     CDS             complement(<1838..2269)
                     /locus_tag="PK198_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40062.1"
                     /translation="MNRIWSLCLLFWGSIALSQPGQLFFGNGLIQQVVPAPDGNYFLL
                     GSSGGANSMVKLLKINPVGDVVWEKSYISGANSYGHGLTQLADGSLLIAGQSGAVALL
                     IKTDAEGNELWKRTYANTTALFDVAPSGNQLLLAGWQDNTGT"
     gene            2405..>3395
                     /locus_tag="PK198_14830"
     CDS             2405..>3395
                     /locus_tag="PK198_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40063.1"
                     /translation="MKKLFTATLPAIAVFLLSTLAASGQWTEIPTPSGPYISGDMEVL
                     NGKVYTSHHSDFKLKCLRTDDYIHWSLLADLPVNSEYGLSQVIVSDQQLYLFSHSRSQ
                     QTASAWTSDDEGQNWQSLHLPSPKPYFFIPFDDALVTASKYTVQHSADNGLHWEQVLT
                     LSDSIWDIKRLDDQTVLLITVSNLYRSADQGLTWEALSSPFDASGMDYPYIYLYSTQP
                     GVFAELDNDHVSTLYRSYDNGTSWTQVSIPYELYDTKVRDLHTVGNKLWGIFSGGAAA
                     SDDGGETWEYRLAPHGTSQFSSYGDTLFAGCSYGFFKSYDQGQSWLSGNLHDQE"
     assembly_gap    3396..3408
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3409..4545
                     /locus_tag="PK198_14835"
     CDS             <3409..4545
                     /locus_tag="PK198_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40064.1"
                     /translation="GELSVSITAMHSHQGKLYLSSDRGLFSTINDGIEWRLHTNHGPF
                     SHFFQQGDTLAFLGRGLRRSFDGGNSWHHIINEDFLNPLFGNYNFARVNEHLFSLAWF
                     DDFVYRSADFGATWEAFPTPSLSTEHIAGSRHALYVNGWGGVYTSVNLGQNFQLINSG
                     LGNSPRVDGVWNTGDHVFLSANYQLWKRDGDQWLPASVGLYNETGDIPYIFNIVGDTD
                     NALLAGKNHALQPELYLSRNGGAFWEGNLAEGLPLFNIHSDFSAILHNNVIYAAGSPS
                     SVFDHHIWKRSVSVTAKEPIKPAAFNLFPNPAQDITWLTLDDVTSIQGRILLHDFTGR
                     LVGQYESTGESIVKIPLHSLSPGMYSVSLLAANGRIIQAKLVVF"
     gene            4676..>5447
                     /locus_tag="PK198_14840"
     CDS             4676..>5447
                     /locus_tag="PK198_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF40065.1"
                     /translation="MKKYLLSICLIFMFAASASAQVRVEPPFWWTGMKTPALQLMVHA
                     PKIAEWHPSISYPGLRVERVVRTENPNYVFIDLLLLDDVKPGKFNIEFKKDGKLMHRQ
                     PYELLQRDAGASERPGFNPSDVMYLITPDRFVNGDPSNDNVTGMREKANRSNKGGRHG
                     GDIAGLLKSLDYIHDMGFTAIWVNPVLENDMQTYSYHGYSTTDFYKVDPRFGSNEDYR
                     KLSLAAQAKGVKMIMDMIVNHCGSEHWWMRDLPAADWLN"
     assembly_gap    5448..5515
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5516..6534
                     /locus_tag="PK198_14845"
     CDS             <5516..6534
                     /locus_tag="PK198_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HRF40066.1"
                     /translation="PYVSTEDRRRFVDGWFVPTMPDLNQRNQLMATYLIQNTIWWVEY
                     AALNGIRMDTYPYPDKNFMSDWSCAVMREYPNLNIVGEEWTENPAWVAYWQGGKKNPD
                     GYSSCLPSLMDFPLQGALRKGLTERDGWNEGLMRLYETLANDFLYADPSQFVIFPDNH
                     DMSRFFSQMNEDFDLFKMGMAYTYTMRGIPQIYYGTEILMKNPGTDDHGIIRSDFPGG
                     WVGDQVNAITGDGLSAQQKEAQDYLRKLGQWRRQAEVIHRGKLMHFLPENGAYVYFRY
                     TEKQRVMVILNKNDQQRQLGLERFSEMIGTARNGKNVTDGKTYSLKNALELPAKTALI
                     LELE"
     assembly_gap    6686..6707
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6708..6888
                     /locus_tag="PK198_14850"
     CDS             <6708..6888
                     /locus_tag="PK198_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRF40067.1"
                     /translation="ESEVTPEASFINDLGADSLDTVELIMEFEKEFNVSIPDDQAETI
                     QTVGLAIEYLEANAA"
     gene            7088..8341
                     /gene="fabF"
                     /locus_tag="PK198_14855"
     CDS             7088..8341
                     /gene="fabF"
                     /locus_tag="PK198_14855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRF40068.1"
                     /translation="MKRVVVTGIGALTPIGNSIEAYLSGLQNGVSGANLITHFDATHF
                     KTKFACEVKNFNIEDIVDRKEARKMDVFSHYATAASDEAVAMAKLPEQIEQLDRDRIG
                     VIWASGIGGLGSLEKEIHDYALGGFIPRYNPFMIPKMIPDIAAGYISIKYGFRGINYA
                     TVSACASSTHAVMAAFDAIRVGRNDVIVCGGSEAAVIQAGMGGFNAMKALSTRNDDYL
                     TASRPFDLDRDGFVLGEGAGGLVLEDLEHALRRGATIYAEVMGAGATADAHHITAPHP
                     EGLGATNVMRMALADAGMTPDDIDYINVHGTSTPLGDIAEVKAIQQVFGEHAYKLNIS
                     STKSMTGHLLGAAGAIEAIACILAIQHQFVPPTINHFTDDPGLDSALNFTFNQMQHRE
                     VKAALSNTFGFGGHNASVIFKAYEG"
BASE COUNT         2009 a         2266 c         1990 g         1873 t
ORIGIN      
        1 tctggcagta aattcaagcg ggcggaggtc attgtgcaaa ttgtaaaacc ctaatccaag
       61 tactgcttcg gcaattttat cgggcacccc cgtggtgttg gcaggcataa cggccgatat
      121 ttcgggcgca gcatcgtcat accgccagga attcagatac cccactgaat acaggccgtt
      181 gtttccctgc ccacagcctt ccaccgaagc ggcagggtag ctttcggagg gatagccagg
      241 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn attaccctgt aaaaagtttc cgcgcctgcg ccgccgccgc cgcgattgaa
      421 taaatcgaaa cggacattgt cgccgtcgca acgcccttca acgatgtatc gcggcccgct
      481 ccagccgggg gcacattccg caggaacaat ttcggccaca acaggatgag aagccccaag
      541 ccgcgcgttg caatcgagtt gcgcccaaat ttcaaaggtt tgagactgca agccctgcat
      601 aggcggcaca gcaaactcta tgctgccgcc gttttgtatg aagggcgcgg aagccgaaac
      661 cacctgcaat gccgggtgaa gccgcacctg gagcagcaag tctccagact cgcccactcc
      721 cctgttggta attgtcacaa aaaagcggct ggtatcgcag cgcaccaagt ccggctgagt
      781 aatgcccacc tcggtaagcg ggcagccgcc gtcgttttgg taggctgtaa aagtgagatt
      841 ggccgaagga tgggcgctgt tactttcaat ggtttgaacg ttgttgcaca aagaccaggc
      901 agactcgtcg ggcgcccgaa ccgtgaactc cgtaaccgca tcttcagtgt ggaaggtaaa
      961 gtttccctcg gcatccgtta tcaggatatg agcagaatgg ttctctgcgc gaacgaacca
     1021 gcccacagcg ggcggctcat ctgcatcggc cacacaattg ccgttgaggt caatgaccac
     1081 ctttccatcc actcgccaga ggcgggcatt gccgtcggct tctgcccgca acagccatgc
     1141 ggacgaggca gcgctgcctc cacccgccgg cgtattggag agaccggcaa gcaaaaattg
     1201 gccgtcaggc agggctgccc ctgtaaaaat cctttcgcgg gaggtgcttt ctccgtagta
     1261 tctgcgccat acttccaatc catcggcggt gacgcgcatg gcaaaaccgt tgatatcggc
     1321 attacccgaa gtggacaaag catttccaca aatcatccag ccgccatcgg gcagagcata
     1381 caatgcgtac ggaaactccc tccaacccgc gctgccgtat attttttgtt ccaacaaaac
     1441 gccctctgct gaaaaatgca acagtgccac ctcttccgca aaggtctggt aaggatttgc
     1501 catccagata ctttcatcgg gcataaaaac catacccaaa ggttcggaga aaaattcacc
     1561 gcttacaggc atgttgtaat ctcgttcctc aaagcctgtg tcgtatgttt tctgccaaat
     1621 aagattaccg gcagggtcta tttttctgat aaaaatgccc ttgaaccccg cgccgatgtc
     1681 attggcccgc cccaacaaca aaaagttgcc gtcggcagta ggaaatatgc gtttggcgta
     1741 tgtctgactt ctcacatcta tcggcttgcg ccattgaaga ataccgnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt gccggtgttg tcttgccagc
     1861 ctgccagtaa aagctgattg ccgctcggcg ccacgtcaaa caaagcggtg gtattggcat
     1921 aagtgcgctt ccagagttca ttgccctccg catccgtttt aatgagcagc gccaccgccc
     1981 cgctttgccc ggcgatgagc aggctgccgt cggcgagttg ggtaaggccg tgaccatagc
     2041 tgttggctcc ggagatataa cttttttccc agacgacatc accgacagga ttgattttca
     2101 gcaatttgac catgctgttg gcgcccccgg aggagccaag cagaaaatag ttgccgtcgg
     2161 gggcaggcac gacttgctga ataaggccgt tcccgaaaaa aagttgtccc ggctgggata
     2221 atgcaatact tccccaaaac agaaggcata gcgaccagat gcgattcatg gctaaacagg
     2281 atttaaaggt tcagagtaaa acaatatctg cgggaaaatt aaaaaacact cttgatatac
     2341 ggctacatta ttttctaaaa aaaccagatt gcgccattat atttacgcgc aaaatccatt
     2401 tgttatgaaa aaactattta cagccaccct tcctgccatc gcagtttttc ttctttccac
     2461 gcttgccgct tcgggccaat ggacggaaat acctactccc tcaggcccct acatttcagg
     2521 agacatggag gtactaaacg gaaaggtata tacttcacac cattcagatt tcaagttaaa
     2581 atgcttgcgt acagatgatt acatccactg gtcgctatta gccgacttac cggtcaattc
     2641 cgagtacgga ctatctcaag tcattgtaag cgaccaacaa ctctacctat tcagccacag
     2701 cagatcacaa cagacagcct ccgcttggac aagcgatgat gaaggacaaa attggcagtc
     2761 acttcatttg ccttcgccaa aaccttattt tttcattcct tttgacgacg cactggtaac
     2821 cgcatcaaaa tataccgttc aacacagtgc cgacaacgga ttgcactggg aacaggtttt
     2881 gacgctatcc gatagcattt gggacatcaa gcgcttagac gatcagacgg tgctgctcat
     2941 taccgtcagc aacctctatc gttctgccga tcaaggactt acatgggaag cattgtcttc
     3001 gcctttcgat gcttccggca tggattatcc ttatatttat ctttactcaa ctcaaccggg
     3061 cgtgttcgcc gaactagata acgaccatgt ttctacgcta tatcgctcct atgacaacgg
     3121 gacatcatgg acgcaggtct ctatcccata cgaattatac gacacaaaag tccgggactt
     3181 gcatacagtg ggcaacaagc tgtggggaat attttcgggc ggcgccgctg cttctgatga
     3241 cggcggagag acatgggaat accgtttggc gccacatggc acgagccaat tttcgagtta
     3301 tggcgatacg ttgtttgccg gctgctcgta tggttttttt aagtcttacg atcaggggca
     3361 gtcttggttg tcggggaatt tgcacgatca ggaacnnnnn nnnnnnnngg cgaattgtcc
     3421 gtctcaatta cagccatgca ctctcatcag ggcaaattgt acctctcgtc cgacaggggg
     3481 ctgttttcta ctataaatga tggtattgaa tggcgattgc acaccaacca cgggcctttt
     3541 tctcattttt tccaacaggg cgacaccctt gcatttttgg gtcgggggct tcggcgctct
     3601 ttcgacggag gaaactcctg gcaccatatc atcaatgaag attttctgaa ccccctcttc
     3661 ggtaactaca attttgcacg ggtcaacgag catttatttt cactcgcctg gttcgacgat
     3721 ttcgtttacc gatctgccga tttcggagct acctgggaag catttccgac gccctccctt
     3781 agtacagaac atatagccgg cagccggcat gcactgtatg tgaacgggtg gggcggtgtt
     3841 tatacctctg tcaatctggg gcaaaatttt caactcatca atagcgggct gggaaacagt
     3901 ccgagggtgg acggcgtgtg gaataccggc gatcatgtat tcttgtcggc aaactatcaa
     3961 ctttggaagc gagacggcga tcaatggctg cctgccagcg tcggcttgta caacgaaaca
     4021 ggagatattc cctatatttt caatattgtc ggagataccg acaacgcttt actggccgga
     4081 aaaaaccatg cgcttcaacc tgagctgtat ttgagccgaa acggaggcgc cttctgggag
     4141 ggaaacttgg cagaaggact gcctttattc aatattcact ccgatttttc tgccattctt
     4201 cacaataatg ttatttatgc cgccggaagc ccatccagtg tattcgacca ccacatctgg
     4261 aagcgctctg tttccgtgac ggccaaagaa cccataaagc cggctgcttt taatttattc
     4321 cccaatccgg ctcaggacat aacctggctg acgcttgacg acgtaacgag cattcagggg
     4381 cgcattttgt tgcacgattt cacagggcgc ctcgtcgggc agtacgaatc aacgggtgaa
     4441 tccatagtaa agattccgct acactcattg tctcccggca tgtacagtgt ttctctgctc
     4501 gccgccaacg gccggatcat acaagccaaa ttggtggtat tttgaaaaca gccgatgcag
     4561 tgcaaaggaa agcatcgcaa cattcgtaaa aaggcaggcg gaagtctgcc tttttccttt
     4621 cccgtttgtt tatctgccgt ttttttctga acgttgcagt caaaccattt ccattatgaa
     4681 aaagtacctc ctttccatct gtcttatttt catgtttgcc gcctccgcaa gcgctcaggt
     4741 gcgcgtggaa ccgccttttt ggtggacggg catgaaaacc ccggctctgc aactcatggt
     4801 gcatgcgccc aaaattgccg aatggcatcc ctccatcagc tatccggggc tgcgcgtaga
     4861 gcgcgtcgtt cgcaccgaaa accccaacta tgtgttcatt gacctgctct tgctggacga
     4921 tgtgaaaccc ggcaaattca acatcgaatt taaaaaagac ggtaagctca tgcaccggca
     4981 gccttacgaa ctgctccaac gcgatgccgg agcctccgaa cggcccggct tcaacccctc
     5041 cgatgtgatg tacctcatta cgcccgaccg cttcgtcaac ggcgacccct ccaacgacaa
     5101 cgtgacaggc atgcgcgaaa aggccaaccg cagcaacaaa ggcggcaggc acggcggcga
     5161 tattgccggg ctgctcaaga gtttagacta tattcacgac atgggtttta ctgcgatatg
     5221 ggtcaatcct gtgctcgaaa acgacatgca gacgtattca taccacggct attccaccac
     5281 cgatttttac aaagtggacc cccgcttcgg cagcaatgaa gactaccgca aactcagcct
     5341 tgcagcgcag gccaaaggcg tgaaaatgat tatggatatg atcgtgaacc actgcggctc
     5401 ggaacactgg tggatgcgcg acctgcccgc cgccgactgg ctcaactnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccct
     5521 tacgtcagca ccgaagaccg ccgccgtttt gttgacggct ggtttgtccc caccatgccc
     5581 gacctcaacc agcgcaacca gttgatggct acctatctca ttcagaacac aatatggtgg
     5641 gtggaatacg ccgctctcaa cggcatccga atggacacct acccgtatcc cgataaaaat
     5701 ttcatgagcg actggagttg cgccgtcatg cgcgagtacc ccaacctcaa cattgtgggt
     5761 gaagaatgga ccgaaaaccc cgcctgggtg gcttactggc aaggcggaaa aaagaatccc
     5821 gacggctact cttcctgcct gccctctctg atggacttcc cgctacaggg cgcgctgcgc
     5881 aagggcctca ccgaacgcga cggctggaac gagggactca tgcgcctgta cgaaaccctg
     5941 gccaacgact tcctttacgc cgatccctcg cagtttgtca tcttccccga caaccacgac
     6001 atgagccgct ttttcagcca gatgaacgaa gatttcgacc tgttcaaaat gggcatggcc
     6061 tacacctaca ccatgcgcgg catcccacag atatattacg gcaccgaaat actgatgaaa
     6121 aaccccggca cagacgatca tggcatcatt cgcagcgatt ttcccggcgg ctgggtcggc
     6181 gatcaggtga acgccattac cggcgacggg ctgagcgcac agcaaaaaga agcgcaggat
     6241 tatttgcgca agttgggcca atggcgccgg caggccgagg tcatccatcg gggcaagctc
     6301 atgcacttcc tgcccgaaaa cggcgcgtat gtctatttca gatacaccga aaaacagcgc
     6361 gtaatggtca ttctgaacaa aaacgaccag caaaggcagt tgggcctgga gcgcttttcg
     6421 gagatgatcg gcacggcgcg caacggcaaa aacgtgaccg acggcaaaac ctattcatta
     6481 aaaaatgcac tggaattgcc tgccaaaacc gcgcttatac tggagttgga atgagccggc
     6541 aatgacacgc caagttactg tctgaaaaaa aagaggcgaa aactttgcgt atcatacagg
     6601 tcttagcttt ctttgcacca aatttttaac aatcctaaag cagattgagt atgtcaaaca
     6661 ttgcagaaag agtaacgaaa atcatnnnnn nnnnnnnnnn nnnnnnncga atcggaagta
     6721 acccccgaag cgagcttcat caacgatctg ggcgccgatt cattggatac ggtagaactg
     6781 atcatggagt tcgagaagga attcaacgtt tccattcccg acgatcaggc cgaaaccatc
     6841 caaacggtcg gattggctat cgaatatctg gaggccaacg ccgcctagta atttcgagcc
     6901 ggcaagtcag gcacacggcg cccgcaaagc atactttcag agttgatttt gcgggcgtca
     6961 gtttttagtt gattttcttt tttgcaaaat cattgcggca ctgccctcat tgcttcagca
     7021 gcagttttta ccataggaca cgttctgaaa caatggttcg gcaggccata aattttgtgc
     7081 agctattatg aagcgagttg tcgttacggg tataggcgcc cttacgccca tcggaaacag
     7141 catcgaagcg tatctcagtg gtttgcaaaa cggcgtcagc ggcgccaatc tcatcactca
     7201 tttcgacgcc acccatttca aaaccaagtt tgcctgcgag gtcaaaaact tcaatatcga
     7261 agacattgtg gaccgcaagg aggctcgcaa aatggacgtt ttcagccatt atgctaccgc
     7321 cgcctccgac gaagccgtgg ctatggccaa gctccccgaa caaattgaac agctcgaccg
     7381 cgaccgcatc ggagtcatct gggcttcggg catcggcggt ttaggctctt tggaaaaaga
     7441 aatacacgat tacgcattgg gcggcttcat tccccgctac aatccgttca tgattcctaa
     7501 aatgattccc gacattgccg ccggctatat ctctataaag tatggattcc ggggcatcaa
     7561 ttacgccacc gtttcggctt gcgcatcttc cactcatgcc gtcatggctg ccttcgacgc
     7621 cattcgcgta gggcgcaacg acgtcattgt gtgcggcggc tccgaagcgg ctgtcattca
     7681 ggccggcatg ggcggtttca atgccatgaa ggccctctct acccgcaacg acgactacct
     7741 caccgcatcg cgccccttcg atttggaccg cgacggtttt gtactcggcg aaggcgccgg
     7801 cggcttggta ctcgaagact tagagcacgc cctgcgcaga ggcgctacca tttatgcaga
     7861 ggtgatgggc gcaggcgcta cggccgatgc gcaccacatt acggccccgc accccgaagg
     7921 tttgggcgcc accaacgtaa tgagaatggc attagccgac gccggcatga cccccgacga
     7981 tattgactac atcaacgtac atggcacctc cacgccctta ggcgatattg ccgaggtgaa
     8041 agccattcag caggtattcg gcgagcatgc gtacaagctc aacatcagct ctaccaagtc
     8101 catgaccggg cacctgctcg gcgcggcggg ggccattgaa gccattgcct gcattctggc
     8161 cattcagcac caatttgtgc ctcctaccat caatcacttc acggatgacc ccgggttgga
     8221 ttctgcgctg aactttacct tcaaccagat gcaacaccgc gaagtaaaag ccgcactgag
     8281 taataccttc ggatttggcg ggcacaatgc ctccgtcatt ttcaaagctt atgagggatg
     8341 attttgacca ctatttaatt cggagccgac ttgaaattcg tattgcgcct ctacaatcgc
     8401 ttcttagctc ccgaccgcga
//
LOCUS       DAORLF010000191         8405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4578_length_8405_cov_2.031497, whole genome shotgun sequence.
ACCESSION   DAORLF010000191 DAORLF010000000
VERSION     DAORLF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4578_length_8405_cov_2.031497"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..762)
                     /locus_tag="PK198_14860"
     CDS             complement(55..762)
                     /locus_tag="PK198_14860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF40069.1"
                     /translation="MKQYIVTGASRGIGRDTALALAAHPSHWVLALSRDLAGLERLEQ
                     AAREAGAEQRLHCLPFDIAQPDMPQLMEVLGQWGKLDGIVHNAGLLINKPFEELDEAD
                     WQRMFQVNFFSPMALTRSLLPLLEAARGAHVLHIGSMGGFQGSSKFNGLSAYSASKAA
                     LANLTETLAEELKSRHIFVNCLSLGAVQTEMLAQAFPGYQAPLSSAEMGGFVAWFAEQ
                     GHRFFNGKILPVSVSTP"
     gene            complement(767..3019)
                     /locus_tag="PK198_14865"
     CDS             complement(767..3019)
                     /locus_tag="PK198_14865"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF40070.1"
                     /translation="MRHNLLIIILLCAVFTVEAQTQEPFRIRIQKTDQPLRLDGALDE
                     DVWGNAQVVSDFYQNFPFDTARASLQTEVRLLFDEHHIYVGAKIYQPRADYIVASLKR
                     DFSSGPTDEFAVNIDPFSDKINGFHFAVSPYNVQREGIIDNGSNINTDWDNRWFCKVS
                     NHDDYWTVEMAIPFKTLRYKTSETAPNWRIGFSRNSVKQNERSAWTQVPRQFNPNNLA
                     FTGLMEWATPPPKPGLNVSLIPYLTGGTSRDFEFKLPSEQRLGIGGDVKIAVTPSLNL
                     DLTFNPDFSQVEVDRQITNLSRFELFFPERRQFFLENEDLFSRFGFPNSRPFFSRRIG
                     IGRGNITRTTTDGQTITVERSVDVPIIAGARLSGKLDNNWRVGLLNMQTARMNDINLN
                     PANYTVGVLQRKVFDRSYVGAVFANKENFIPSSGGGYDIDKNAYSRVAGLEYNLFSKD
                     NRWEAELFYHRSFSPGNPADPNSAALFLGHFTRNWRLFFPTQYIGSGFRSDAGFVPRT
                     GFFSTSPGLTRSFFPKNQKRVVSYGGELNSQFIFNTHGGFQGTDRSFSAQGFVEFAGQ
                     SALTAGWNSDYTYLFFAFDPTNSGGAQLPAGTDYVYNSLQAAFNSDLRRNFYYDISLQ
                     HGQYFNGDITQVSGAFNYRWQPYGILALRYNYNDIRLPAPYNSARFWLVGPRAELAFS
                     RSLFFSLFLQYNTQINNVNVNSRLQWRFRPVSDLFIVYTDNYFSENFFAMPTPKNRTL
                     LLKWTYWLNL"
     gene            complement(3021..3581)
                     /locus_tag="PK198_14870"
     CDS             complement(3021..3581)
                     /locus_tag="PK198_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF40071.1"
                     /translation="MAFTESNMLPIGTIAPPFALPDTVSGNTITFDDIAGEKATIVMF
                     LCNHCPYVIHVNEEIVRIALDYQPKGVGLVGISSNDVENYPDDAPDKMQEHARQVGYT
                     FPYLYDESQETARAYDAACTPDFYVFDGSRRLVYRGRLDNARPKTDTPLTGADLRAAL
                     DAVLEDREVNSVQYPSGGCNIKWKKD"
     gene            3695..4777
                     /locus_tag="PK198_14875"
     CDS             3695..4777
                     /locus_tag="PK198_14875"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRF40072.1"
                     /translation="MQEYPNDLLPDTEAQPPNMRVSRIILPVLIGVGVVAYLFWRQFD
                     AQEFARIDWTVRLAVLLGLAVLLAAVRHLAYAARLRVLSNGAFSWWKCIQLIFLWEFS
                     SAVSPTSLGGSAVAFFLLSREKLSLAKTTTIVLYTVVLDSAFMLLTLPFLYLIFGAGI
                     MRPGAATFAEAGAWGHFFILAYLGMMAYTGLFYYGLFIGPQRVKRFLGSLTSWSWLKR
                     FRRRAIILSNEFILASGELRNEPSSFHLQSLAHTFVAWATRFFLLSAIIVAFIPTIPV
                     DVVTQMELYARLQTMFFVIAFSPTPGGAGLIELLFGGFLTDYVSNGTVSTIISSVWRL
                     ISYYVYLLAGAVLIPGWLRKTASKSS"
     gene            4856..6052
                     /locus_tag="PK198_14880"
     CDS             4856..6052
                     /locus_tag="PK198_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRF40073.1"
                     /translation="MNQITHPPVVQLRFDDTAAAFAHKSDGQLRKSYLLFQMMNNPAL
                     VNAGARIAEWAFRLHLPVIWLVKNTIYQQFCGGETISEVKQVIQALGRHRIKTILDYG
                     VEAKASEEDFERTANHLQQTLEVAREDSFIHIVSMKLSGLFRFALLEKVSRMDALNDA
                     ETAEWQRGIDRVDRICGAAAASGISIHVDAEHSWIQGAVDRIAWIMSERYNREQPTII
                     NAVQMYRKDRQAFLEASLEHAQQHGYLCAVKLVRGAYMEKERARAAAMSYPDPIHATL
                     EDTHRAYNRALEFCLSHLGDMYVCNASHNEASCRLMAELMQRYHIPNDHPLTATAQLY
                     GMSDNISYVMARAGYNVEKYLPYGPVREVIPYLIRRTRENTSAGGQMSRELGLLHREL
                     LRRKEK"
     assembly_gap    6154..6277
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6278..6920
                     /locus_tag="PK198_14885"
     CDS             <6278..6920
                     /locus_tag="PK198_14885"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF40074.1"
                     /translation="KIGILGSGVVARTLGNGFLKHGYEVTMGTRDASKLTEWHQGEGA
                     KAKIGTFAEAAYKADIIVLAVSGKAAESALELAGEPVLEGKTIIDVTNPIAPEPPVNG
                     VLKFFTTLDDSLMERLQRRFPAANFVKAFNCVGSALMVNPHLKGGAPSMFICGNDANA
                     KDDVHYILDQFGWETEDMGGVEAARAIEPLCILWCIPGFQRNQWSHAFKLVKG"
     gene            complement(6917..7468)
                     /locus_tag="PK198_14890"
     CDS             complement(6917..7468)
                     /locus_tag="PK198_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40075.1"
                     /translation="MRGIIVMTLAAVLSLLTTCHSESGTAAASGAAPYFDLKKYLESE
                     ITRLEKAQGSVVKTVSVNGKEEQQTLQQLDYRSELAPLLDADINRPAWQGKYRVDSTR
                     VSPGEWTLTYTAADEKPPVRTLSVHFQDNAVESILIERTAHTLMAGSSQQLSYRPAAG
                     FEVATVQKAVFMQERKIFVGVAY"
     gene            complement(7465..>8405)
                     /gene="gpmI"
                     /locus_tag="PK198_14895"
     CDS             complement(7465..>8405)
                     /gene="gpmI"
                     /locus_tag="PK198_14895"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRF40076.1"
                     /translation="LAYDLLVHGAGEHSNDALATIQQRYAAGETDEFLQPIVCVGGAG
                     QGQAQIQPGDAVLCFNFRTDRPREITTVLSQTDMPEHGMNTLPLHYVTMTRYDDTYKN
                     VEVIFQKDDVSNTLGEVLQNAGKTQVRIAETEKYPHVTFFFSGGREEPFEGERRLLAP
                     SPKVATYDLKPEMSAYELTDMIVADIRENTPDFICLNYANADMVGHTGVFEAAIKAAE
                     TVDRCMERLIATALEFGYEAIVIADHGNSDYMVNEDGSPNTAHTMNPVPCIYVSGRAA
                     GVRVKPGKLGDIAPTILYLMGLPAPAAMTGDILIER"
BASE COUNT         1811 a         2160 c         2397 g         1913 t
ORIGIN      
        1 catctgaaca tcaggggaag ccggccttta tcaaacccca aacaagacaa aacctcaggg
       61 cgtactcacc gaaaccggca aaatcttgcc attgaaaaag cgatgcccct gctctgcaaa
      121 ccaggccacg aaaccgccca tttctgcact gctcagcggc gcctgatagc cggggaacgc
      181 ctgtgccagc atctcggttt gtacggcgcc caaggacaga cagttgacga aaatatgcct
      241 cgatttcagc tcttcggcca gcgtttcggt caggttggcc aaggcggctt tcgaggcact
      301 gtatgcactc aggccgttga atttgctgct gccctgaaac ccgcccatgc tgcctatgtg
      361 taagacgtgc gctccacggg ctgcttcgag caggggcaac aagctgcgcg tgagggccat
      421 tggactgaaa aaattgacct gaaacatgcg ctgccagtcg gcctcatcca gttcctcaaa
      481 gggcttgttg atgagcagtc ccgcattgtg aacgatgccg tccaatttgc cccattggcc
      541 cagcacttcc atcagttgcg gcatatcggg ttgggctatg tcgaagggca gacagtgcag
      601 gcgttgctcg gcgccggcct cacgggcggc ttgttcgagg cgttccagcc cggccagatc
      661 gcgcgacagc gcaagtaccc aatgcgaggg atgcgctgcc aatgccaggg cggtatcgcg
      721 gccaatgccc cggcttgcgc cagtgacgat gtattgtttc attttcttac aagttgagcc
      781 agtacgtcca tttcaacagc aaagttctgt ttttgggcgt cggcatggcg aagaagtttt
      841 cagaaaaata gttgtcggta taaacgatga agaggtcgga aacgggcctg aacctccatt
      901 gcaggcggct gttcacattc acgttgttga tctgagtgtt gtactgcaaa aacaagctga
      961 aaaacaggct gcgcgagaag gccagctcgg ccctcggccc cactaaccag aaacgggcgc
     1021 tgttgtaggg cgcaggcagg cggatatcgt tgtaattgta gcgcagcgca aggatcccgt
     1081 agggttgcca acggtaattg aacgcgccgc tgacctgcgt gatgtcgccg ttgaaatact
     1141 gcccgtgttg caaagaaatg tcgtagtaaa aattgcgtcg caggtcggaa ttgaacgctg
     1201 cctgaagtga attgtaaaca taatcggtac cggcgggcaa ttgcgctcca cccgaattgg
     1261 tgggatcaaa ggcaaaaaac aggtaggtgt aatcactatt ccagccggca gtgagcgcgc
     1321 tttgacctgc aaattctaca aaaccctgcg cagagaagct ccggtcggtg ccctgaaaac
     1381 cgccatgggt gttaaaaatg aattgtgaat tcaactcgcc gccataagaa acgacgcgct
     1441 tttggttttt gggaaagaaa ctgcgcgtga ggccgggcga ggtagagaaa aagcccgttc
     1501 ggggaacgaa gcccgcgtcg ctgcgaaaac cggaaccgat gtattgggta ggaaaaaaca
     1561 gccgccagtt gcgggtaaaa tgccccaaaa acaaggctgc cgagttgggg tccgccggat
     1621 tgcccggcga gaaggagcgg tggtaaaaca gctctgcctc ccagcgattg tctttggaaa
     1681 acaggttgta ttctaatccc gccacccggc tgtaggcgtt tttgtcaatg tcgtagccgc
     1741 cgccggagct tggaatgaag ttttctttgt tggcaaaaac agcgcccaca tacgagcggt
     1801 cgaacacctt gcgctgcaat acgcccaccg tataattggc cggattgagg ttgatgtcgt
     1861 tcatgcgagc ggtctgcata ttgagcaggc ccacgcgcca gttgttgtcc aatttgccgc
     1921 tcaatctcgc gcctgcaatg atgggcacat ccaccgagcg ctctacggtg atggtttggc
     1981 cgtcggtggt ggtgcgggtg atgttgccgc gcccgatgcc gatgcggcga ctgaaaaaag
     2041 ggcggctgtt ggggaaaccg aagcggctga agaggtcttc attttccaaa aagaactgcc
     2101 tgcgttcagg gaagaacagt tcaaaacggc tcaggttggt gatttggcgg tccacttcca
     2161 cctgcgaaaa atcggggttg aaggtgaggt cgaggttgag cgaaggcgtg acggctattt
     2221 tgacgtcgcc gccgatgccc agcctttgct cggagggcag tttgaactca aaatcgcggg
     2281 aagtgccgcc tgtgaggtag ggaatgagag atacgttgag gccgggtttg ggcggcggtg
     2341 tggcccattc catcaggccg gtgaaagcga ggttgttggg gttgaattgc cggggcacct
     2401 gcgtccaggc cgagcgctcg ttttgtttta ccgagttgcg cgagaagccg atgcgccagt
     2461 tgggggcggt ttcggaggtt ttgtagcgga gggttttgaa ggggatggcc atttccaccg
     2521 tccagtaatc gtcgtggttg ctcaccttgc aaaaccagcg gttgtcccag tcggtattga
     2581 tgttgctgcc gttgtcaata atgccctcgc gttgcacgtt gtagggcgac acggcaaaat
     2641 ggaagccgtt gatcttgtcg gaaaagggat caatattgac ggcaaattcg tcggtaggac
     2701 cagaggagaa atcgcgtttc agcgaggcaa cgatgtagtc ggcacgcggc tggtatatct
     2761 tggcgcccac atatatatgg tgttcgtcga agagcaggcg cacctcggtt tgcaggcttg
     2821 cccgcgccgt gtcgaagggg aaattttggt aaaaatcgct tactacctgc gcgtttcccc
     2881 agacatcttc atccaatgcc ccatccaatc gcagcggctg gtctgttttc tgaatgcgga
     2941 tgcggaaggg ttcctgcgtt tgcgcttcga ctgtaaacac tgcacatagc aggatgataa
     3001 tgagtagatt gtgccgcata tcagtctttt ttccatttga tgttgcagcc gccgctggga
     3061 tattgcaccg agtttacttc acggtcttcg agcaccgcgt ccaatgcagc ccgcaggtcg
     3121 gcgccggtca ggggcgtatc tgttttaggg cgggcattgt ctaagcggcc gcgatagacc
     3181 aagcgtcgcg agccgtcgaa gacataaaaa tcgggggtgc aggcggcatc ataagcgcgg
     3241 gcagtttcct gcgattcgtc gtacagataa ggaaaagtgt agcccacttg gcgggcgtgt
     3301 tcctgcattt tgtcgggcgc gtcgtcgggg taattttcca cgtcgttgga actgatgccc
     3361 accaagccca cgcctttcgg ctgataatcg agagcgatgc gcacaatttc ttcgttgacg
     3421 tgaatcacat acgggcaatg gttgcagagg aacatcacga tggtggcctt ttcgccggct
     3481 atatcgtcaa aggtaatggt gttgccactc acggtatcgg gcagagcgaa gggcggcgcg
     3541 atggtgccga tgggcaacat attggattct gtaaatgcca ttttgttctg aagttgttga
     3601 aaagaatacg acattaacgc ctgccggggt gtgaggttga aggcttcgtc aaggtttctt
     3661 acctttatag ccgaattgca acactcatta ttacatgcaa gaatacccaa acgatctgct
     3721 tccggacacc gaagcgcaac cacccaatat gcgggtgtcc cgtatcattt tgccggtact
     3781 catcggggta ggagtggtgg cgtatttatt ctggcggcag tttgacgcac aagagtttgc
     3841 ccgtattgac tggaccgtcc gtctggcagt gctgttgggg ctggcggtac tgttggcggc
     3901 agtgcggcat ttggcttatg cggcccgatt gagggtgttg tcgaatgggg cattttcgtg
     3961 gtggaaatgt attcagttga tttttttatg ggaattttcc tctgctgtgt cgcccaccag
     4021 tttgggcggt tcggcggtgg cgttttttct gttgagccgg gaaaagctga gccttgccaa
     4081 aaccaccacc attgtattat atactgtagt attggacagc gccttcatgt tgctcaccct
     4141 gccgtttttg tacctgattt tcggggcggg catcatgcgt ccgggggctg ccacttttgc
     4201 ggaagcaggg gcctggggtc attttttcat cctggcctat ctgggtatga tggcatatac
     4261 agggctattt tattacggtt tgttcatcgg gccgcagcgg gtcaagcgtt ttctggggtc
     4321 gctcacatcc tggtcttggt tgaagcgatt ccgccgcagg gccatcattt tgagcaatga
     4381 atttatttta gcttccgggg aattgcgcaa cgaaccttca tcctttcacc tccaatctct
     4441 ggcgcatacc ttcgttgcct gggctacacg tttttttcta ctcagcgcca tcattgtggc
     4501 attcatcccc actatcccgg ttgatgttgt gacccaaatg gagttgtacg cccgcctgca
     4561 aaccatgttt tttgtcattg cgttcagccc cacgcccggc ggtgcgggtt tgatagaact
     4621 gcttttcggc ggttttttga cggattatgt atctaacggt acggtttcca ccatcatttc
     4681 cagcgtatgg aggcttattt catactacgt ctatttgttg gccggcgcgg tattgatccc
     4741 tggctggctg cgaaaaacag cctcgaaatc gtcataaatc actccttcca ttgacgagcg
     4801 tcattgcctg atgttgggaa cagggctaaa tttcctgcac caaataaaat caggtatgaa
     4861 ccagatcacg catcctcctg tcgtgcaact tcgcttcgac gatacggctg ccgcatttgc
     4921 acacaaaagc gacggccaac tgcgcaaaag ctatttgctt ttccaaatga tgaacaaccc
     4981 ggccttggtc aatgcaggcg cccgcatagc agaatgggct tttcggctgc acctgccggt
     5041 gatttggttg gtcaaaaaca ccatttacca acagttttgc ggcggagaaa ccatcagcga
     5101 ggtcaaacag gtcattcaag cgctggggcg gcatcgcatc aaaaccatcc tggactatgg
     5161 ggtcgaagcc aaagcctccg aagaagactt tgagcgaacg gccaatcatt tgcagcaaac
     5221 gctggaagtt gcccgcgaag acagttttat ccacatcgtg agcatgaaac tcagcggctt
     5281 gttccggttt gccttgttgg aaaaagtgag ccggatggat gccctcaacg atgccgaaac
     5341 agccgaatgg cagcgcggca tagatcgggt ggatcggata tgcggagccg ccgccgcatc
     5401 aggtatttcc attcatgttg acgccgaaca cagttggata cagggcgctg ttgaccgcat
     5461 tgcctggatc atgtcagagc gctacaaccg cgaacagcca accatcatca acgcagtgca
     5521 aatgtatcgc aaagaccggc aggcttttct ggaagcatca ctcgaacacg cgcagcaaca
     5581 cggctatctc tgtgccgtca aattggtgcg cggcgcttac atggaaaaag agcgcgcccg
     5641 ggctgccgcc atgagctacc ccgatccgat acacgccacg ctggaagaca cacaccgggc
     5701 atacaaccgg gcgctcgaat tttgcctcag ccacctcggc gatatgtatg tgtgcaacgc
     5761 atcccacaac gaggcaagtt gccgactcat ggccgagctg atgcagcgct accacatacc
     5821 caacgaccac ccgctgacgg ctacagccca actctatggc atgagcgata atatctctta
     5881 tgtgatggcg cgggctgggt acaatgtgga aaaatacctg ccctacgggc ctgtgcgcga
     5941 ggtaattccg tacctcattc gccgcacgcg ggaaaacacc tctgccggcg ggcagatgag
     6001 ccgcgaactg ggattgctgc acagagagtt gctgcgaaga aaggaaaagt gagggcttgg
     6061 gagagaaggg accggtgtgc agtcggcggt aggcggcgct aagcgtgagg gaactcggaa
     6121 ccttgaacgc gaaacccgaa acaagcctgt tccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa aataggcatt ctcggatcgg
     6301 gcgtagtggc ccgcacactc ggcaacggct ttctcaaaca cggctacgaa gtgacgatgg
     6361 gcacccgcga tgcctccaaa ttgaccgaat ggcatcaggg cgaaggtgca aaagccaaga
     6421 tcggcacctt tgcggaagcg gcttataagg cggatatcat cgtactcgcc gtatcgggca
     6481 aggcggccga atcggcgctg gaactggccg gcgaacccgt tctggagggc aaaaccatca
     6541 tagatgtgac caatccaata gcccccgaac cgcccgtgaa cggcgtactg aagtttttca
     6601 ccactttgga cgactccctc atggagcgcc tgcagcggcg tttcccggcg gccaatttcg
     6661 tcaaagcctt caattgcgtg ggcagcgcgc tcatggtcaa tccgcacctc aaaggcggcg
     6721 cgccttccat gttcatttgc ggcaacgacg ccaatgccaa agacgatgtg cattacatcc
     6781 tcgaccagtt tggctgggaa acggaagata tgggcggtgt agaagcagcc cgtgccattg
     6841 agccgttgtg tattttgtgg tgcattccgg gatttcagcg caaccaatgg tcgcacgctt
     6901 ttaagctggt gaagggctaa tacgccacac ccacgaaaat cttacgctcc tgcataaaca
     6961 ccgccttctg cacggtggcc acctcgaaac cggcggcagg gcggtaggat agttgttggc
     7021 tcgaccccgc catcagcgta tgcgcggtgc gctcgatgag aatggattcc acagcattgt
     7081 cctgaaaatg tacggacaag gtgcgcaccg gcggcttttc gtcggcagcg gtgtaggtca
     7141 gggtccattc gccgggggaa acccgggtgg aatccacgcg gtatttgccc tgccaagccg
     7201 ggcgattgat gtcggcgtcg agcaggggcg ccagttcgga gcggtagtcc aactgttgca
     7261 gggtttgttg ctcctcttta ccgttgacgg acaccgtttt cactacggag ccttgtgctt
     7321 tttccaagcg cgttatttcc gattccaaat attttttcag atcgaaatac ggcgctgctc
     7381 ctgatgcagc agcggttccc gactcgctat ggcaggtcgt caacaagctg agaacggccg
     7441 ccaatgtcat tacaataata cccctcatcg ttcaatcagt atatcgcccg tcatagcggc
     7501 aggtgcgggc aatcccatca aatacaggat ggtaggagct atgtcgccca atttgcctgg
     7561 ctttactcga acgccggctg cacggccgct aacgtaaata caaggcacgg gattcatggt
     7621 gtgcgccgtg ttggggctgc cgtcctcgtt caccatatag tcggagttgc cgtgatcggc
     7681 gatgacaatg gcttcgtaac caaactccag cgcggtggcg atgaggcgct ccatacagcg
     7741 atccaccgtt tcggctgctt taatggctgc ctcgaacacg ccggtatggc ctaccatgtc
     7801 ggcattggcg tagttgaggc aaatgaaatc gggcgtattc tcgcgaatgt ccgccacaat
     7861 catatcggtg agttcgtaag cgctcatttc tggcttcagg tcgtaggtgg ccactttggg
     7921 agagggcgcc agcagtcggc gttcgccctc aaacggctcc tctctgcctc ccgaaaagaa
     7981 gaaagtgaca tgcggatact tttccgtttc ggcaatgcgc acctgcgttt tacctgcatt
     8041 ttgaagcact tcgcccagcg tgttcgacac gtcgtctttt tgaaaaatga cctctacatt
     8101 tttgtaggta tcatcgtagc gcgtcatggt cacatagtgc aggggcagcg tattcatacc
     8161 gtgttcaggc atgtcggttt ggctgagtac ggtggtgatt tcgcgggggc ggtcggtgcg
     8221 gaagttgaag cacaacaccg cgtcgccggg ctgaatctgc gcctgcccct gtcctgcgcc
     8281 tccgacacaa acgatgggtt ggagaaactc gtcggtttcg ccggctgcat agcgctgctg
     8341 tatggtggcg agggcgtcgt tgctgtgttc gcctgcaccg tgaacgagca ggtcgtaggc
     8401 caatt
//
LOCUS       DAORLF010000192         8391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4587_length_8391_cov_1.935461, whole genome shotgun sequence.
ACCESSION   DAORLF010000192 DAORLF010000000
VERSION     DAORLF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_8391_cov_1.935461"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PK198_14900"
     CDS             complement(<1..1471)
                     /locus_tag="PK198_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF40077.1"
                     /translation="MASRRDFIKISGLGLLGLGVTGGAWSVLRASTAWEDLVAEGIRF
                     SPTYCEVCFWKCAGWVYKNEKGDIQKVVGNDDDPHCNGRLCPRGTGGTGMYHDPDRLT
                     TPLIRVTENGKQTFRKASWDEALNWIADKMKQTKEQYGPESVALFSHGTSGDHFEHLF
                     KAFGSATLAGPSYAQCRGPRDAGFKATYGAGVGSPEPTDIRDTRCLVLIGSHIGENMH
                     NSQVQEMAEAIDKGVDIITVDPRFSTVAGKSKHWLPIKPATDLALLLSWMHVLIYENL
                     YDKAYIEQYATGFEQLKQHVRRFTPEWAYGITTIQPDQIRATARLMAAAAPAVIVHPG
                     RHVTWYGDDSQRSRAIAILNALLGAWGRRGGFFLKEKASLPEYPYPPYPQPRWTWRDT
                     LDGRYALADEAVTNTLIEASIPKEGQAARIKGWVITGTNLPQAIPEKGKMLQAIDALD
                     FVVAIDTMPMEVTGFADVVLPECTFFERYDNLRNAPFREP"
     gene            complement(1640..2188)
                     /locus_tag="PK198_14905"
     CDS             complement(1640..2188)
                     /locus_tag="PK198_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF40078.1"
                     /translation="MYKFVKILLPAVFAAALLSGCQSGNKDIRQEARQSIEGTGAVTP
                     QNPNPTDPAAQNSPVPTGPTTEVVFTETEFDFGSVKEGEKVSHTYKFKNAGKEPLVIS
                     NAQGSCGCTVPKWPRDPIAPGKTGEIVVEFDTKGKAGDRNQKVTLTANTNPPQTFLSL
                     KGKVEGDPNAQPQTAPTMQVNQ"
     assembly_gap    2325..2363
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2521..2826
                     /gene="trxA"
                     /locus_tag="PK198_14910"
     CDS             2521..2826
                     /gene="trxA"
                     /locus_tag="PK198_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF40079.1"
                     /translation="MKKKVSFNDLINGDKPVLVDFSAEWCGPCKAMAPILREVAGTIG
                     EKASIVKIDVDQNRALAQQMQIQGVPTFVLFKNGQVKWRQSGMQSAHRLAGIIEQYA"
     gene            3022..3597
                     /locus_tag="PK198_14915"
     CDS             3022..3597
                     /locus_tag="PK198_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRF40080.1"
                     /translation="MQSIVDFISQPWHWAVSGAMIALTLFILTWMGRSFGVSMTFRNA
                     CTLAGAGKKTPFFNIDMKEEWWRMAFVLGGLVGGFIAVTFFRSPEPVAISEATVAHLA
                     DWGLNYPAADAKGLGFVPTEVFNFGSVKGIILAVVGGFLVGFGARYADGCTSGHAISG
                     LSHLQLPSLITVIGFFIGGLLMTHVIMPLLF"
     gene            3625..>3997
                     /locus_tag="PK198_14920"
     CDS             3625..>3997
                     /locus_tag="PK198_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRF40081.1"
                     /translation="MKNIAFFAVGIFFGIVMTKSEAISWFRIHEMFRFESIHMYGIIG
                     TAVILGAGIIFAMKKLKIKTLTGNLVSYTPMELRIPRHLLAGTAFGLGWAITGCCPGP
                     MYVLIGQGYWIIVLVILSAILG"
     assembly_gap    3998..4158
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4159..4854)
                     /locus_tag="PK198_14925"
     CDS             complement(<4159..4854)
                     /locus_tag="PK198_14925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF40082.1"
                     /translation="MKPLKISIVTVTYQAAAILEKTIQSVIFQKKNDLLELEYWIIDG
                     GSTDGTLDLIQKYQSHLTGWISEKDKGIYHAMNKGISNASGDWIGFMNAGDQFNNLEV
                     LYNLFTQVRPAAEVIYGNYHIEYQSFAKPKLVPADLNNFWQGMLLNHQSVFIKTELAK
                     KYPFDLTYRLAGDYAQLYNLYMKGCIFQYVNIFVARFADGGESAKRKITYIREIEDIV
                     FKHEAPVLAKRIHF"
     gene            5067..6512
                     /locus_tag="PK198_14930"
     CDS             5067..6512
                     /locus_tag="PK198_14930"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRF40083.1"
                     /translation="MSIALGTLNILFIAPAILTTEELGLINIVRDVSLLAAPFIYLGA
                     IDLILKFFPIFRSEEKKHNGFFFLIGLHVTIGNVLFLLLVLFFYHDISAYYSARSPLF
                     KDYLPLVIPMAVLNVYSMVLTYYISNFSRIAVPAIFNDLIPKAGTIALILAYHYQWLP
                     FSGIVWGLAGLNALILAGHVWYVRHLGQLHLRPDFSFIDKPLFRSMATFAGFGILISI
                     GYQLLARIDLVMVASLSTLHATGVYSVAFFIINIIEVPKYAMSKIANPIIAEHWQAER
                     LEEIASLYKKGALHQLIAGAGLFLAIWASVDHLFELMPNGDQYAAGKYVIFILGLARL
                     MDMAGGFSIEIIHMSKHYRYLFFTLLFMAALNLSCHSLLIPVLDMKGAALATLISFVV
                     YSWLKIALIWWKFRMQPFSRQTLAVLAGGLLVFAAGQVIPDTGIPLADIAIKSSLSML
                     LFAGFILFFKISPEVNQLAVEVWEQLRRRIK"
     gene            6550..7896
                     /locus_tag="PK198_14935"
     CDS             6550..7896
                     /locus_tag="PK198_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRF40084.1"
                     /translation="MQPVFVFDAGLFKLPIIHSIRKLNQPIKLLHMKILNSFLALFLS
                     ATMLTAQSNCLRDTLIRLEDMPVFNSAVSAAVYNGEIYTFGGFTTTGGPIQNSSFKYN
                     VSNGVWSQLAPMPSPRGEVGVAELNGIIYCMGGWTGNIESDRNHAYNIATGTWSVKAN
                     LPFRMASNDAVAYNGKIYLVGGIVENPASFFIEYTPETNLYRVLSPPLRTRRNAKLIV
                     YQDRFYLLGGQYDHGLDRFVTDQVEEYNPQTEVWTQKAPMPLGLQHYGASLINGKIHV
                     ICGASTGWPVPNLVLHPTHYVYDIATDTWSQKSDLPFSLFALETAVYNDELYIFGGNP
                     SSSSFSNQCHKITCVEWEGLVQQRETLAEHTGIKIYPNPVYENLTINTGIYATLRGYS
                     IRVCNDQGQKVHEIQLTQQLHDIDVKKLGRSGAYIVYFVSPEGRIAETKKVIVVVK"
     gene            complement(7903..>8391)
                     /locus_tag="PK198_14940"
     CDS             complement(7903..>8391)
                     /locus_tag="PK198_14940"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF40085.1"
                     /translation="VAVCRRAAQWLAADAGAQVLDVGSGAGKFCMVGAAVCSGGFFTG
                     IEQNERLHEAACEIQARLDFGNLAFLHGNVLDVDMRPFSGFYFFNAFHENLMPSDTDD
                     MQQRRMRYRQYTHYLREQLSELPAGTRLATYHSFLQEVPDSYQLREAAFDGYLRLWEK
                     AR"
BASE COUNT         1847 a         1968 c         2191 g         2185 t
ORIGIN      
        1 acggctcgcg gaagggggca ttgcggaggt tgtcgtagcg ctcgaagaag gtacattccg
       61 gcagtaccac atcggcaaaa ccggtcactt ccatcggcat ggtatctatg gctaccacaa
      121 agtcaagtgc gtcaatggct tgcaacattt tgcccttttc gggaatggcc tgcggcaggt
      181 tggtaccggt gatgacccag cctttgatgc gggccgcctg gccttctttg ggaatggacg
      241 cctcaatgag ggtgttggtg acggcctcat cggccagcgc gtagcggccg tcgagggtgt
      301 cgcgccaggt ccagcggggc tgagggtagg gcgggtaggg gtattccggg agggaggcct
      361 tttctttgag gaaaaagccg ccgcgacggc cccaggctcc aagaagtgcg ttgagaatgg
      421 cgatggcgcg agagcgctgg gagtcgtcgc cgtaccaggt aacatgccgg ccggggtgaa
      481 caatgacggc cggggcggca gcagccatga ggcgggctgt ggcgcggatt tggtcgggct
      541 gaatggtagt gatgccgtag gcccattcgg gcgtgaagcg gcggacgtgt tgtttaagtt
      601 gttcaaaacc agtggcgtat tgctctatat aggctttgtc gtacaggttt tcgtagatca
      661 aaacgtgcat ccaagagagg agcagggcca ggtcggtggc gggtttgatg gggagccaat
      721 gtttggattt accggccacg gtggagaagc gcgggtccac ggtgatgatg tccacacctt
      781 tatctatggc ttcggccatt tcctgcacct gactgttgtg catgttttcg ccgatgtggg
      841 agccgatgag cactaagcag cgcgtatcgc ggatatcggt gggttcgggc gaaccgacgc
      901 cggcgccgta agtagcttta aaacctgcat cccgcgggcc gcggcattgt gcataggaag
      961 gcccggcgag ggtggccgaa ccaaaggctt tgaagagatg ctcgaagtgg tcgccgctgg
     1021 tgccgtggct gaaaagcgcc acactttcgg ggccgtattg ctctttggtt tgcttcattt
     1081 tgtcggcaat ccagttgagc gcttcgtccc aggaagcttt tcggaaggtc tgtttgccgt
     1141 tttcagtaac tcggatgagc ggagtagtga ggcggtcggg atcgtggtac atgccggtgc
     1201 cgccggtgcc gcgcggacag aggcgcccgt tgcaatgcgg gtcatcatcg ttgcctacta
     1261 ctttctggat atcgcctttt tcgtttttat acacccagcc ggcgcacttc cagaagcaga
     1321 cttcgcagta ggtaggagaa aaacgaatgc cctcggctac gaggtcttcc caggctgtgg
     1381 aggctcgcag tacgctccat gcgccgcctg tcacgcccaa gcccagaagc cccaggccgg
     1441 aaattttgat aaaatcgcgt cttgatgcca tgatgaatga tggtttcgtc atgtgttgat
     1501 tcgagtgcga aataaggagg tttagagagg ggggcagatg atttcggtca ggaggccggg
     1561 gtgatttatg tcaggcagaa aaacaaaagc cggacaggag caattcccat ccggcttttg
     1621 aagacagatt gagtaccaat tactggttca cctgcatagt aggagccgtt tgcggctgtg
     1681 cattcgggtc gccttccact ttgcctttca gagagaggaa agtttgcggc ggattggtat
     1741 tggcggtgag cgtcactttc tggttgcggt cgccggcttt gcctttggtg tcgaattcaa
     1801 cgacgatttc gccggttttg cccggagcga tggggtcgcg cggccatttc ggaacggtgc
     1861 aaccgcaact gccttgtgcg ttggagataa cgagcggctc tttgcctgca tttttgaatt
     1921 tgtaagtgtg gctcactttt tcgccttctt tcacagatcc gaaatcaaat tcagtttcgg
     1981 tgaaaaccac ttcggtggta gggccggtag gaacgggcga gttttgagcg gccgggtccg
     2041 tcggattggg gttttgcgga gtcacagctc cggtgccctc aatgctttgg cgtgcttcct
     2101 gacgaatgtc tttgttgccg ctctggcaac cagacaacaa tgctgctgcg aaaacagccg
     2161 gaagaagaat tttgacaaat ttgtacattg cttattggtt ttaaatttaa tgtggaacaa
     2221 agaaaatccg ccgcaaaggt actgaacagc cgggcgcttg cgctgttaac ggctcggcaa
     2281 ttttcgttaa caaagggtta acgatttgta ccttgcgccg ccagnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnattagca gcgcccggta tgacaaaagc ggcttcgcac
     2401 ctgacggtgc gaggtatagt tgcgaaagtt tatccgcttt ttgtgacgca catcacaacg
     2461 cagcgccccg gcaaggctgc atctttgcgg catctttttg atttttaaaa catcattacc
     2521 atgaagaaga aagtgtcttt caatgattta attaatggcg acaaacccgt tttggttgat
     2581 ttttccgccg aatggtgcgg cccctgcaaa gctatggctc ccatcctccg cgaagtagcc
     2641 ggcacgatcg gcgaaaaagc atctattgta aaaattgacg tggatcaaaa ccgcgcactg
     2701 gctcagcaaa tgcaaattca gggcgttcct actttcgtgc ttttcaaaaa cggccaggtg
     2761 aaatggcgtc agtcgggcat gcagtcggcg caccgcctgg caggtattat tgagcagtat
     2821 gcgtgagggg cggttgtgcg gtaggcggta tgcagtcggc ggtaggcagt cggcggtgct
     2881 aagctcgagc gaaacgcgac cagcagcttg cagccagcag ccgttccaac ccggaacccg
     2941 gaatacagtt ggaggcttgt agccaaaaat gatttacctt tgtattgttt tacatccaat
     3001 tgaccacaac aaatctaaat tatgcaatcc atcgttgact tcatcagcca accctggcat
     3061 tgggcggtat cgggcgccat gattgcgctg acgctcttta ttctgacttg gatgggccgt
     3121 agtttcggcg tgtccatgac ttttcgcaat gcgtgtacgc tggccggcgc cggcaaaaaa
     3181 acgccctttt tcaatattga tatgaaagag gagtggtggc gcatggcctt cgttttgggc
     3241 ggcttggtgg gcggcttcat tgccgttacc ttcttccgca gtccggagcc ggtagccatt
     3301 tctgaagcca ctgtggcgca cttagccgat tggggtttga attacccggc ggccgatgcc
     3361 aaaggcttgg gttttgtgcc taccgaggtg ttcaacttcg gcagcgtcaa aggcattatt
     3421 ctggcggtag tgggcggttt cctggtgggc ttcggcgcgc gctatgccga cggctgcact
     3481 tcgggtcatg ctatcagcgg gttgtcgcac ctgcaattgc cctcgctcat cacggtgatc
     3541 ggctttttca tcggcggcct gctgatgacg cacgtcatta tgcccctgct gttttaattc
     3601 acccatccaa aacgattcac acgcatgaaa aatatagcat tttttgccgt cggtatcttc
     3661 ttcggcatcg tcatgaccaa gtcggaagcc atttcctggt ttcgcatcca cgagatgttc
     3721 cgtttcgaga gcatccacat gtatggcatc atcggtacgg cggttattct gggcgctggt
     3781 atcatttttg ccatgaaaaa actgaaaatc aaaaccctca ccggcaattt ggtgagctat
     3841 acgccgatgg agctgcgcat tccgcgccac ctgctggcag gcacggcctt tgggttgggc
     3901 tgggccatca ccggctgctg ccccgggcct atgtatgtgc tcatcggaca aggctactgg
     3961 atcatcgtac tggtaatact gagcgccatt ttgggtannn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnga aatgaatcct tttggccaaa acgggggctt catgtttaaa
     4201 aacaatatcc tcaatttcac ggatatatgt gattttgcgt ttggctgatt cgccgccatc
     4261 tgcaaatctt gccacgaaga tgttcacata ttgaaaaatg cagcctttca tatacaggtt
     4321 gtatagctgg gcataatctc cggccaagcg gtatgtcaaa tcgaaaggat atttttttgc
     4381 caactccgtt ttgatgaata cactttgatg gttgagcagc ataccttgcc agaaattgtt
     4441 caaatcggca ggaacgagct ttggctttgc aaatgattga tactctatat ggtagttgcc
     4501 gtatatgacc tctgcagccg ggcgaacttg cgtaaacagg ttgtatagca cttccaaatt
     4561 attgaactgg tcaccggcat tcataaatcc gatccaatcg ccgcttgcat tcgatatgcc
     4621 cttattcatg gcatggtaaa tacccttgtc tttttcgctg atccatcccg tcagatgaga
     4681 ctggtatttt tgtatcagat caagggttcc atctgttgag ccaccgtcta tgatccaata
     4741 ttccaattcc aaaagatcat tttttttttg aaaaattacg ctttggatgg ttttttccag
     4801 tatagcagcg gcctgatagg ttacagtaac gatactgatt tttagcggtt tcatgcctca
     4861 aaagtacaat gtttgagggg aactgaccgg cgtttttgtt ttcattttga ctcgcccgct
     4921 gaggcgactc tgctttgcgc atatctttcg tatcatttaa tacctttgcc cttgagtcgc
     4981 ggatgatcgg gctgttattg tcatatactc aaatttagat gggcgtagtc aaaaggcaaa
     5041 gtatcaaaca aagtattgtg gtgtacatgt ctattgcgct gggcaccctg aacatacttt
     5101 ttattgcccc cgctatactg accaccgaag aactgggcct catcaatatt gtacgggatg
     5161 tgtctctatt ggcggcgcct tttatttatc tgggcgcgat agacctgatc ctgaaatttt
     5221 ttccaatttt cagaagcgaa gaaaaaaaac acaatggctt ttttttcctc atcgggctgc
     5281 acgtcaccat aggcaatgtg ctttttctgc tgttggtgct gtttttttac catgacattt
     5341 cggcctacta ctcggcgcgc tcgccgcttt ttaaagacta tctccctttg gtcattccga
     5401 tggctgtact caatgtttac agcatggttt tgacctatta tatctccaat ttcagccgga
     5461 tagcggtgcc ggccatcttc aatgatctga ttcccaaggc aggcaccatt gcgctgattc
     5521 tggcatatca ttatcaatgg ctgccttttt ccggcatcgt ttggggtttg gcgggtctca
     5581 atgcgctcat cctcgccggg catgtatggt atgtgcggca tctggggcaa ctgcacctgc
     5641 ggcccgattt ttcgttcatt gacaagcctt tgttcaggag catggccact tttgccggct
     5701 tcggtatcct catcagcatt ggctaccaac tgctggcccg tattgatctc gtaatggtgg
     5761 cctccttgtc cacattgcac gctacggggg tgtattctgt ggcatttttt atcatcaaca
     5821 tcattgaggt gcccaaatac gccatgtcga aaatagcaaa ccccatcata gcagagcact
     5881 ggcaagccga gcgtttggag gaaattgcat cgctgtacaa aaaaggcgcg ctccatcagt
     5941 tgattgccgg cgcaggtttg tttttggcta tatgggcttc ggtggatcat ttgtttgaac
     6001 tgatgccgaa cggcgaccaa tacgccgcgg ggaagtatgt gatttttata cttggtttag
     6061 cccgcttgat ggacatggcc ggcggtttca gcatcgagat catccacatg tcgaaacact
     6121 accggtattt gttttttacg ctgctgttca tggctgcgct caatctcagt tgccactcat
     6181 tgctcattcc ggttttggac atgaaaggcg cagcgttggc taccctcata tcgtttgttg
     6241 tgtatagctg gctcaaaata gcgctgatat ggtggaagtt caggatgcag ccgttttcgc
     6301 ggcaaacgct cgctgtactg gccggcggtc tgcttgtttt tgcggcaggg caagtcatcc
     6361 cggacacagg gattccgttg gcagacattg ccataaaatc ctctctgtcc atgcttttgt
     6421 tcgcagggtt tattttattt tttaagattt ccccggaggt caatcagttg gctgtggaag
     6481 tttgggaaca gttaaggcgc agaataaagt agccctgatt tggtacatga aagcgatatt
     6541 tgagtatatt tgcaacctgt ttttgttttt gacgccggtt tattcaagct cccaatcatt
     6601 cactccatta gaaaacttaa tcaaccaata aaattattac acatgaaaat cttgaactct
     6661 tttttagctc ttttcttatc ggctacgatg ctgactgctc aatccaattg cttgagagat
     6721 acgctcatca ggctcgaaga tatgccggtt ttcaacagcg cggtatcggc ggcagtttac
     6781 aacggagaaa tatacacatt cggaggcttt acaactaccg gtgggcctat tcaaaactcg
     6841 tctttcaagt acaatgtgag caatggtgtc tggagccagt tggcccccat gccttctcca
     6901 cgaggagagg tgggggtggc tgaattgaac ggaatcatct attgtatggg aggctggacg
     6961 ggcaatatcg agagcgatcg aaatcacgcc tataacatcg caacaggcac ctggagtgta
     7021 aaggccaacc tgcctttcag aatggcgagt aatgatgcag tggcctataa cggcaaaatt
     7081 tatctggtag gcggtatcgt tgagaatccg gcttcctttt tcattgaata cactcctgaa
     7141 acgaatttgt atcgggtgct ttccccgcca ctgagaacga gaaggaatgc caaactgatt
     7201 gtgtatcaag acaggtttta tctgctgggc ggccagtacg accacggatt ggaccggttt
     7261 gttaccgatc aggtagaaga atacaacccc caaactgaag tatggacgca aaaagccccc
     7321 atgccactgg gattgcagca ctatggagcg tcattgatta atggcaaaat tcatgtcatt
     7381 tgtggcgcca gtactggatg gccggttccc aatttggttc tgcatcccac tcattatgtg
     7441 tatgatattg ccacagacac ttggtcgcaa aagagcgatt tgccgttcag tttgtttgcg
     7501 ctcgaaactg ccgtttataa tgatgaacta tatattttcg gaggcaatcc aagcagttcg
     7561 tctttttcca atcaatgcca taaaatcact tgtgtggagt gggaagggct tgtacaacag
     7621 agagaaacgc tcgctgagca tacgggaata aaaatctacc ccaatcctgt ttatgaaaac
     7681 ctgacaatca acacgggcat atacgcgaca ttgagaggat acagcatcag ggtgtgtaat
     7741 gatcaggggc agaaagtcca tgaaatccag ctcacgcaac aattgcatga tattgatgta
     7801 aaaaaactgg gtagaagcgg cgcttacatt gtgtatttcg tgagtcctga agggagaata
     7861 gcggagacca aaaaagtcat tgtcgtggtc aaataacccc ctctacctcg ccttctccca
     7921 aagcctgaga tacccatcga aagctgcttc gcggagttgg tagctgtcgg gtacctcttg
     7981 gaggaaactg tggtaagtgg ccaatcgcgt tccggcgggc agttccgaaa gctgctcccg
     8041 gaggtagtgg gtatattgcc tgtaccgcat tcttctctgc tgcatgtcat ctgtatctga
     8101 gggcatcaga ttttcgtgga aagcattaaa aaaataaaaa ccgctgaacg gccgcatgtc
     8161 cacatccagt acattgccat gcaaaaacgc aagattgccg aaatccaacc gcgcctgaat
     8221 ctcgcaggcc gcctcgtgca ggcgctcatt ttgctctata cccgtaaaaa agccccccga
     8281 acaaacagcg gcgcccacca tgcaaaactt gcccgcgccc gaacctacgt ccaacacctg
     8341 tgcgccggcg tccgcagcca gccattgggc agccctgcgg cataccgcta c
//
LOCUS       DAORLF010000193         8384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4591_length_8384_cov_2.903350, whole genome shotgun sequence.
ACCESSION   DAORLF010000193 DAORLF010000000
VERSION     DAORLF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_8384_cov_2.903350"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..627)
                     /locus_tag="PK198_14945"
     CDS             complement(151..627)
                     /locus_tag="PK198_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HRF40086.1"
                     /translation="MKKTLRYILYALPVLLIVAQAFRIDKSVPEYDRSQDFLALMQAP
                     QDVADLMRAACYDCHSYESKYPWYAEIAPVSWWVKQHINEGREHLNLSLYGSWSPKKR
                     AHKIEECIESLKEGWMPLKSYTRGHPEARLSESQRMAISAWLAGLPDARFESEHDH"
     gene            738..1295
                     /locus_tag="PK198_14950"
     CDS             738..1295
                     /locus_tag="PK198_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40087.1"
                     /translation="MKYFLPVFVALISVAAPAFAQSYFTAGGLRMGTDWGLTLQQRVA
                     KKTSVELILQTNFRRKEGMFTLLGEQHIPLISKRFNLYYGGGIHKGWYTEKPLYGVDE
                     PEISGPFGITAIGGAELTIGRLNLSYDVKPALNISGGSNVIYLQSGVSARYVIAKKPF
                     WEEQKKASKKRHKKKKTSRNKLRLW"
     gene            complement(1300..1755)
                     /locus_tag="PK198_14955"
     CDS             complement(1300..1755)
                     /locus_tag="PK198_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40088.1"
                     /translation="MRFITTALFVAAIMALSFSCRQDKPDSISETIDKQSVELLCENL
                     QDSEDAPSASVYLKTGDRKTKIAGISGQCSGINSGEYANYGMPNGTISAVGSWWAGSG
                     DYLYAVLEGAKVVVYQGFADEMQEDAGFHYKPIVVYDGKKCTFVNTSEN"
     gene            complement(1831..3300)
                     /locus_tag="PK198_14960"
     CDS             complement(1831..3300)
                     /locus_tag="PK198_14960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRF40089.1"
                     /translation="MKKILVANRGEIALRVMRTARKMGIKTVAVFSEADREAPHVRFA
                     DEAVCIGPPPSNRSYLLGDKIIETALSLGVDGIHPGYGFLSENAAFAQKVQDAGLIFI
                     GPDPYAIEVMGNKLAAKDAVKGYDIPMVPGAESAISDVEEAQKIARQVGLPILIKAAA
                     GGGGKGMRIVEHEQDLPAQMERAISEAQSAFGDGAVFIEKYIASPRHIEIQVLADSHG
                     NVVHLFERECSVQRRHQKVVEEAPSSVLTPEVRAAMGQAAVMVAKACRYKGAGTVEFL
                     IDEQLNFYFLEMNTRLQVEHPVTELISGVDLVEQQIRIARGETLSFTQDELHIRGHAL
                     ELRVYAEDPYDNFHPSIGKLEVYRRPKGEGIRVDDGFEEGMDIPIYYDPMIAKLITYG
                     ETRSAAIHRMLEAIEMYQIEGVATTLPFGKFVCQHEAFTSGHFDTHFVQHYFNIEALK
                     ESNREEGLYAALLALQLYTEEKKKLHIPEVGGSNWKYFR"
     gene            complement(3345..3833)
                     /locus_tag="PK198_14965"
     CDS             complement(3345..3833)
                     /locus_tag="PK198_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40090.1"
                     /translation="MEQLNEIWAWLAGKTAAFGPLEWGLTATGGMGLLVLPFLIGMSR
                     RQRKVVSRVPTHLAFYSYQSAPLGRDALLKIKNTRDHVVLLSVAIKGSDEIEVKNASA
                     GHELLSGKVYGIMLEAAGKDRMLPDFELVLTYMDAGRNVWKQSFFPDLQGAGRAKRIK
                     KA"
     assembly_gap    4013..4128
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4129..4945
                     /locus_tag="PK198_14970"
     CDS             <4129..4945
                     /locus_tag="PK198_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRF40091.1"
                     /translation="ARKMRFEINVLDAAEAGARIPEADPNDVSVGYGGLPDRDGNVTL
                     DACVMDDKGNSGAVCFLKNIKHPVSVARKVMENTPHNMICGSGALEFALSQGFIEEDL
                     LTEGSRKAWQEWLKEKKYQPVINIERHDTIGILAMDKDGRLSGACTTSGLSYKMSGRI
                     GDSPIIGAGLFVDNEIGAACATGLGELVTQTLGSFLIVELMRMGYSPQAACEEAVNRI
                     IRRAEAAGVTDYQAGFLALDKNGEHGAFSVVQGFNYALYQNNKNIVLEAGHKL"
     gene            4999..5736
                     /locus_tag="PK198_14975"
     CDS             4999..5736
                     /locus_tag="PK198_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40092.1"
                     /translation="MKNSLFVIAALLVLGFLGCKNESNSNAASDSTADQPVKVDGKIP
                     LFDSTAHAQEMAKRQKETEANVISQDNFTQKAPAGAMAVGKVENTLFEKNDALASAIK
                     AYLTGKNIVITYDNGQYGYNLLDLNGDGAQDALVYLPGSNFCEGNKCTVLVAKGDAAG
                     KFKVHSMIQHLGLPVLVCDASETKGWKDIVTQVFDSENRAASVKIAFDGTKYTEAVSK
                     AKALDKAVLVSGFLMNGDKTSMNLAAN"
     gene            5748..>6676
                     /locus_tag="PK198_14980"
     CDS             5748..>6676
                     /locus_tag="PK198_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF40093.1"
                     /translation="MEPLQRIPTLSDIQETHESIAYLIHRTPVLKCRSIDDISGARIF
                     FKCENFQKVGAFKMRGAAGALLRLSETDRQRGVATHSSGNHAQAVARAAKVLGIPAYI
                     VMPENAPQIKRKATEGYGAQIVPCAPTVAARETMLEAVLARTGAVFIHPYNDYNVIAG
                     QATAAKELLDDVADLDAVIAPIGGGGLMSGTALSTRYLSPGTKIFGAEPTEVNDAYRS
                     LKAGKIEVNETTNTIADGLRTNLGEKTFDIISQHIDDIFTVSEEEIVQAMRLVWERMK
                     IIIEPSCAVPLAAVLRHREVFAGRRVGIILSGG"
     assembly_gap    6677..6805
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6887..7972
                     /gene="fbaA"
                     /locus_tag="PK198_14985"
     CDS             6887..7972
                     /gene="fbaA"
                     /locus_tag="PK198_14985"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRF40094.1"
                     /translation="MNISTQTRFRPGVLHGDEVTELFRFANENNFALPAVNVIGTNSV
                     NAVLETARAVNSPVIIQFSNGGSVFFAGKSLSNENQKAAVLGGISGAMHVHQLAEAYG
                     VPVILHTDHCALNLLPWVDGLLDAGERFFEKNGYPLYSSHMLDLSEEPIEENMHISAK
                     YLDRMKALGMTLEIELGVTGGEEDGVDNSDVDSSKLYTQPSEVAYAYEQLSKVSHRFT
                     VAAAFGNVHGVYKPGNVKLQPIILKNSQDYVRAKFGLSEALPVNFVFHGGSGSSREEI
                     REAIEYGAIKMNIDTDMQWAYWDGVRGYYESKKGYLQSQIGNPEGDDKPNKKYYDPRV
                     WLRKGEETFVTRLKSAFEDLNCIGRNA"
BASE COUNT         1991 a         2164 c         2054 g         1930 t
ORIGIN      
        1 ccacagaatg attgcttttt tcataggccg aatgaaacgg ttctgaaagt ctccaattgc
       61 ctgaatgaaa cgcccggcgg aaatacaaaa gtatttgccg gccatccgat aacggcacaa
      121 agatgaaaaa aatatatatg tttgcggcga ttaatgatcg tgctccgact caaatctcgc
      181 atcgggcaga ccggccagcc atgcactgat ggccatgcgc tgagattcgc tgagccgggc
      241 ttcgggatgg ccgcgcgtgt aagacttcag gggcatccaa ccttctttga gcgattcgat
      301 gcactcttct attttgtgtg cgcgcttttt cggagaccag gagccgtaca aagacaggtt
      361 gaggtgctcg cgcccttcgt tgatgtgttg tttgacccac caggaaaccg gcgcaatttc
      421 ggcgtaccag ggatacttgc tttcgtagga atggcagtcg tagcaggcgg cgcgcatcag
      481 gtcggcaaca tcctgcggcg cttgcatgag tgccagaaaa tcctggctgc ggtcgtattc
      541 cggcacgctt ttgtcaatgc ggaatgcctg agcgacgatg agcagtaccg gcaaagcgta
      601 caaaatgtag cgaagtgtct ttttcattgt tgtttgtttt tatagtgagt tgcaaatgta
      661 gtgaaccaag taatttttcc aactttatac tcgtttgtgg ccgtacaact ttccacttga
      721 ctcaacatgt cattgccatg aaatatttcc ttcctgtttt tgtcgctctg atttcggtag
      781 cagcgcccgc ctttgcacag tcctatttta ctgccggcgg cctgcgcatg ggtaccgact
      841 ggggacttac ccttcagcaa agagtggcca aaaaaacgag cgtagaactg attttgcaaa
      901 ctaattttag acgcaaagaa ggtatgttca ccttattggg agaacaacat attccactga
      961 tttccaaacg cttcaatctc tattacggcg ggggcattca caaaggctgg tacaccgaaa
     1021 aaccgctgta cggggtggac gaaccggaaa tatcggggcc tttcggcatc actgccatcg
     1081 gaggagcgga actgaccatt ggccgcctga acctgagcta cgatgtaaaa ccggcgctca
     1141 atatttccgg cggttccaac gtgatctacc tgcaatcggg ggtgtcggcg cgctatgtca
     1201 ttgctaaaaa acctttttgg gaagagcaga aaaaagcaag caagaagcgg cacaaaaaga
     1261 aaaaaacaag tcgcaacaaa ctccggttgt ggtaatccct caattctccg aggtattcac
     1321 aaaggtgcat tttttcccgt cgtacaccac gatgggctta taatggaagc cggcatcctc
     1381 ctgcatttca tctgcaaagc cttgataaac aacgaccttt gccccctcta aaactgcgta
     1441 caaataatcg cccgaaccgg cccaccaact acccaccgcg ctgatcgttc cgttgggcat
     1501 accgtaattg gcgtattcac cggaattgat tccgctgcac tgtccgctga tgccggcaat
     1561 tttcgttttt cggtcgccgg ttttaagata aaccgatgcc gagggcgcat cctcactgtc
     1621 ctgaaggttt tcacataaca gttcaaccga ttgcttatca atggtttccg atattgaatc
     1681 cggcttatcc tgacggcagg aaaaagacag cgccatgatg gcagcaacga acaacgcggt
     1741 tgtgataaat ctcataatat gatctctttg ggttggcaaa agtaggcaat tcgccgagga
     1801 gtgaaagcct gttgaatatc catctctgcc ttaccggaaa tatttccagt tggagcctcc
     1861 cacctctggt atgtgcagtt tcttcttttc ttcggtataa agttgcagcg ccagcaaagc
     1921 ggcatacagc ccctcctcgc ggttgctttc tttgagggcc tcaatattga agtagtgctg
     1981 cacgaaatgc gtatcaaaat ggccggaagt gaacgcctca tgttggcaca caaatttgcc
     2041 gaaaggcaaa gtagttgcca cgccctctat ctgatacatc tctatggctt ccaacatgcg
     2101 atgaatggcc gcactgcggg tttcgccata agtaatcagt ttggcgatca tgggatcgta
     2161 ataaatggga atgtccatgc cctcctcaaa gccgtcgtcc acgcgaatgc cctcgccctt
     2221 tggccgccgg tacacctcca atttgccgat gctggggtga aaattatcgt aagggtcttc
     2281 cgcatacacg cgcagttcga gcgcatggcc gcgaatgtgc aattcatcct gcgtgaaaga
     2341 gagggtttcg ccgcgcgcta tgcgaatttg ctgctccacc aagtccacgc cggagatgag
     2401 ttcggtgacc gggtgctcta cctgaagccg cgtattcatt tcgaggaagt aaaaattgag
     2461 ttgctcgtca atgagaaact ctaccgtgcc tgcacctttg taacggcatg ctttggctac
     2521 cataaccgcc gcctgcccca tcgctgcgcg cacttcggga gtgagcacgg aggagggtgc
     2581 ttcttctact actttttggt gacgccgctg aacgctgcac tcgcgctcga agaggtgaac
     2641 gacgttgccg tgcgagtcgg ccagcacctg tatttcgatg tggcggggcg aggcgatgta
     2701 tttttcaata aacacagcgc catcgccaaa agcagactgg gcctcgctga tggcgcgctc
     2761 catctgtgca ggcaggtctt gctcgtgttc tacgatgcgc attcctttgc ccccgcctcc
     2821 ggccgccgct ttgatgagaa tgggcaaacc tacctgccgg gcaattttct gggcttcttc
     2881 cacatccgaa atggcggatt cggcgccggg taccatagga atatcgtagc ctttgacggc
     2941 gtctttggcg gccagtttat tgcccatcac ctctatagcg taggggtcgg ggccaataaa
     3001 aatgagacct gcgtcctgca ctttttgagc aaaagctgca ttttcactga ggaagccata
     3061 gccgggatga atgccgtcaa cgcccagaga cagcgccgtt tcgatgattt tatctcccag
     3121 caaataagac cggttggatg gcggagggcc tatgcagaca gcttcgtcgg caaagcggac
     3181 atgaggcgct tcgcggtcgg cctcagaaaa caccgctacg gttttgatgc ccattttgcg
     3241 ggcggtgcgc attactcgca gggctatttc gccccggttg gctaccaaaa tttttttcat
     3301 atccttgtgt tggtttttca gcccgcttct gcactcgggg cactctacgc ttttttgatt
     3361 ctttttgcac ggccggcccc ctgcaaatcg gggaagaagg attgcttcca cacattgcgc
     3421 ccggcatcca tgtacgtcag taccaattca aaatcgggca acatgcggtc tttgcccgca
     3481 gcttccagca tgatgccata caccttgccg gaaagcagtt cgtggcccgc cgaggcgttt
     3541 ttcacctcta tctcatccga acctttgatg gccacactga gcagtaccac atggtcgcgg
     3601 gtatttttga ttttcagcaa ggcatcccga cccagcggcg ccgactgata cgaataaaaa
     3661 gcgaggtggg tgggcactcg cgacacgact ttgcgttgcc ggcggctcat tccaatcaga
     3721 aatggcaaaa cgagcaggcc catgccgccg gttgccgtca gaccccattc caacgggccg
     3781 aaagcagccg ttttgcctgc caaccaggcc catatttcgt tcaattgttc cattgtgttt
     3841 cgttcagggg cggaaagtta gtgcaaagaa tgccaaaggg aggaataact ctttgctttt
     3901 tgccaaattt gctgcttcat tcatcaacaa acacaaacaa tgagcaatcg ccgcgacttt
     3961 tttcgctaca ccctggttgc agcagccggc gccgccctca caggctgcgt tcnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cgcggaagat
     4141 gcggttcgag atcaacgtcc tggatgccgc agaagccggc gcccgcattc ctgaagccga
     4201 ccccaacgac gtcagcgtgg gctacggcgg cctgcccgac cgcgacggca acgttacttt
     4261 ggacgcttgt gtaatggacg acaaaggcaa ttcgggagca gtatgttttc tcaaaaacat
     4321 aaaacacccc gtatcagtgg cgcgcaaagt gatggaaaat accccgcata acatgatctg
     4381 cggcagcggc gcgctcgaat ttgctctttc ccagggattt attgaggaag acctgctcac
     4441 tgaaggctcc cgcaaagcct ggcaggaatg gctcaaggaa aaaaaatatc agcccgttat
     4501 caacattgaa cggcacgaca ccatcggaat attagctatg gacaaagacg ggcgcttgtc
     4561 gggggcctgc accaccagcg gcctgtctta taaaatgagc ggcagaatcg gcgactctcc
     4621 catcatcggg gcaggtttgt ttgtggataa tgaaatcggg gcagcctgcg caaccgggct
     4681 gggagaattg gtgacacaaa cattaggctc ttttctcatt gtagaactga tgcgcatggg
     4741 ctactcgccg caggcagcct gcgaggaggc cgtcaaccgg attatccgca gggcggaagc
     4801 tgccggtgtt actgattatc aggcaggttt tctggcactc gacaaaaacg gagagcatgg
     4861 cgccttttcg gtggtgcagg ggttcaacta cgctttgtat caaaacaaca aaaacatcgt
     4921 tttagaggcc gggcacaaac tttgatttat tagctgaaaa tgcgtttctt tgcgccccga
     4981 aaaaaaatct gtaataccat gaaaaattca ctgtttgtca ttgctgcact tttggtgctt
     5041 ggctttctgg gctgcaaaaa cgagagcaac tccaatgccg cctccgactc tactgccgat
     5101 caacctgtaa aagtggatgg aaaaattcct ttgtttgact ccacggcaca cgcccaggaa
     5161 atggccaaga ggcaaaaaga gacagaagcc aatgtcatct cccaagacaa ctttacccaa
     5221 aaagctccgg caggggccat ggccgtgggc aaggttgaaa acacgctctt tgagaaaaac
     5281 gacgcactcg cttctgccat caaagcatac ctcaccggca aaaacatcgt catcacttac
     5341 gacaacggcc aatacggtta taatctgttg gacctcaacg gcgacggcgc acaagatgcc
     5401 ttagtgtatc ttccaggctc caatttctgt gagggcaaca aatgtaccgt gctcgtagcc
     5461 aaaggagatg ctgccggcaa attcaaagta cattctatga ttcagcatct ggggctgccg
     5521 gttctggttt gcgatgcttc cgaaacaaaa ggctggaaag acattgtcac gcaggttttt
     5581 gattccgaaa accgagctgc ttccgtcaaa atcgctttcg atgggaccaa atacactgag
     5641 gcggtttcca aagccaaagc attggacaag gccgttctcg tctctggttt tctgatgaat
     5701 ggcgacaaaa cgagcatgaa cctcgccgcc aattaatcac acccggtatg gaaccgttgc
     5761 aacgcatccc cacgctgtcc gacattcagg aaacgcacga gagcattgct tacctgattc
     5821 atcgcacgcc ggtgctcaaa tgtcgcagca ttgacgacat cagcggcgcc agaatttttt
     5881 tcaagtgcga aaattttcaa aaggtgggcg ctttcaaaat gcgtggcgca gcaggagccc
     5941 tgcttcgact gagcgaaacg gaccggcaaa gaggcgttgc aacgcattcc tccggcaatc
     6001 atgcccaagc ggtggcgcgc gcggccaagg ttttgggcat accggcttac atcgttatgc
     6061 cggaaaatgc cccccagatc aagcgaaagg cgaccgaggg ctacggcgct caaattgtgc
     6121 cctgcgcgcc taccgtggca gccagagaaa ccatgctgga ggctgtattg gcccgcaccg
     6181 gcgctgtttt tattcacccc tacaacgact acaatgtcat tgcaggacag gccacggctg
     6241 ccaaggaatt gctggatgat gtggccgatc tcgacgccgt tatagcgccc attggcggcg
     6301 gcggactgat gagcggcaca gccctcagca ctcgctacct ctctcccggc actaaaatat
     6361 tcggcgccga acccactgaa gtcaatgacg cttaccgctc gctgaaagca ggcaaaattg
     6421 aggtcaacga aaccaccaac accatcgccg acggcctgcg taccaacctg ggcgagaaga
     6481 ccttcgatat tatttcgcag catatagacg acatcttcac cgtatccgaa gaagagatcg
     6541 tacaggccat gcgcttggta tgggagcgca tgaaaatcat catcgaacct tcctgcgcgg
     6601 tgccgctggc tgccgtacta cgccatcggg aggtctttgc cggtcggcgc gtgggcatca
     6661 ttctcagtgg cggcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnngggag atggccattg tttgtttaag ctccgctgcc
     6841 acttgcgcga ttttcatatc tttgccccat caaaaacatt atcatcatga atatttcaac
     6901 ccaaacccgt tttcgccccg gcgttctgca cggcgacgaa gtaacggaac tgttccgttt
     6961 tgccaacgaa aataactttg cattgccggc cgtcaacgtg atcggcacca actcggtaaa
     7021 tgccgtattg gaaactgccc gcgccgtcaa ttcgcccgtc attattcagt tttccaatgg
     7081 gggttcggtg ttttttgcag gcaagagcct ttccaacgag aaccaaaaag cagccgtgct
     7141 gggcggtatt tccggcgcca tgcacgttca tcagttggcc gaagcttacg gcgtaccggt
     7201 catattgcac accgatcact gcgcgctcaa tcttctcccc tgggtggatg ggctgctgga
     7261 cgccggcgag cgattttttg agaaaaacgg ctatccgcta tacagttctc acatgctcga
     7321 tttatcggaa gaacctattg aagaaaacat gcacatcagc gccaaatatt tagatcgcat
     7381 gaaggcgctg ggcatgacgc tggaaattga actgggcgtc accggcggcg aagaagacgg
     7441 cgtggataac tcggatgtgg acagctccaa actttataca cagccttccg aagtggcgta
     7501 tgcttatgaa caactgagca aggtcagcca tcgctttacc gtagcggcag cgttcggcaa
     7561 tgtacacggg gtttataagc ccggcaatgt gaagttgcaa cccattatcc tgaaaaactc
     7621 acaggactat gtgcgcgcaa aattcggcct ttccgaagct ctgccggtca attttgtctt
     7681 ccacggcggt tcgggttctt cccgtgaaga aatccgcgaa gccattgagt acggagccat
     7741 taaaatgaac atagacaccg acatgcaatg ggcctactgg gacggcgtgc gcggatatta
     7801 tgaatcgaaa aaagggtatt tgcaatcgca aatcggcaat cccgaaggcg acgataagcc
     7861 caataaaaag tactacgatc cgcgtgtgtg gctgcgcaaa ggcgaagaaa cttttgtaac
     7921 ccgcctcaag tctgcatttg aagacctgaa ctgcatagga cgcaacgctt gattgtaatg
     7981 cccctctatt ggggctttat gccaattaat aaaatgagcc atcctgaact ttgaatacgt
     8041 tcaaaattca ggatggctca tttcttttta cggcttagta gtggggcaaa aataaagtgt
     8101 tcattttctt taggccaaag gattagataa gacacttaaa atgaaacgtt tacttatcta
     8161 atcctttggc gaaaatgatc actttatttt tttatcgccc cttacagcac gcatttctcg
     8221 tacaatgcac tacccgcgcg tcagtactac ccgccgcgtc gcatccggca ggccggcaga
     8281 tttcactttc accaagtaca ttccgttttg taagcctttc aaatcgaggc gttccagtgt
     8341 attctgcact tcgtccaatt cactgaattg cagcagcttg cctt
//
LOCUS       DAORLF010000194         8379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4592_length_8379_cov_2.084575, whole genome shotgun sequence.
ACCESSION   DAORLF010000194 DAORLF010000000
VERSION     DAORLF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_8379_cov_2.084575"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1881)
                     /locus_tag="PK198_14990"
     CDS             complement(22..1881)
                     /locus_tag="PK198_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF40095.1"
                     /translation="MSKLTIKSGKAELNLHKSSTLVGVKTTGEAGEQEFVDEEVYKNL
                     GGFSVVTLKTEGESVDDKLDEVRRLDEVETGAHVYYAEGSNRPLIATGEIFITFQPGV
                     SEPEQAIVLEEFHLELTERRAPDRIAVRVTAQSPNPLKVAAALQHISMVKLAEPDLDT
                     VLDEYAFAAPPDNLLGQQWHLSNSGLLPDTNYPIRKGADAKVMDAWKRLDGLGASSVV
                     VAVIDNGFDTSHPDLKDKIFKPFDLWNQSSQLIQGDLRYSHGTPCAAIAIAASNGQGV
                     VGVAPNAKFMPVSGTSYAVRATEQMFDYCVANGADIISCSWGATDAAFALGTDKENAI
                     AKAARQGRNGKGCVVLFAVGNEGYDFVSFYAAHPDVIAVAASTSKDEHAPYSNRGRQV
                     SVCAPSNGDWPIISARAWWDPGVDGESGNYKYWRDGKARGQYYKHFGGTSASTPLVAG
                     VCALMLSANPDLTAKEVKEILQNTADKIGSPSDYVNGHSLKYGYGRVNADRAVAEALR
                     RKESAKPVTEVPAVVGSGKGLFRFDVQKQAPEGWGVQIGVFYDYGNVLIQAERMKSLF
                     GQPAIVHISELQGKTAYRVVIGALKNINDAKALADKMKAGGVNGFVKNLKDMA"
     gene            complement(1886..2458)
                     /locus_tag="PK198_14995"
     CDS             complement(1886..2458)
                     /locus_tag="PK198_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRF40096.1"
                     /translation="MEIVFATGNAHKISEVSELLPEGIRLIGLPEIGCPTDLPETSDT
                     IPGNALQKARYVYEHFGVNCFAEDTGLEVYALHGEPGVYSARYAGDDKDPAANTRKLL
                     DKLAAHSDRSARFRTVIALILEGREYLFEGIVEGRIAETPAGDGGFGYDPVFVPEGET
                     RSFAQMSNKEKGRISHRGRAVVKLLDFFQS"
     gene            complement(2616..3104)
                     /locus_tag="PK198_15000"
     CDS             complement(2616..3104)
                     /locus_tag="PK198_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF40097.1"
                     /translation="MSKFAKKRGKADPNISTASLPDIIFMLLFFFMVVTKMRDSELKL
                     TIITPFASELTKLEEKSLVATIYVGRPIEKYRPIYGTKPQIQLNDKFAKVGQEEADIR
                     YFMESFRTKVPESRRPQITTSLRVDGNVTMGIVADVKTALRKSGQLKVNYSAKRRPDA
                     TL"
     gene            complement(3150..>3641)
                     /locus_tag="PK198_15005"
     CDS             complement(3150..>3641)
                     /locus_tag="PK198_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF40098.1"
                     /translation="FLLLIFFLVTTTIVEDKGITVKLPPWSEEEPDITQLKTRNVYSV
                     LVNAQNQLLVRNQPMSIRDLRKNAVEFISNPYGREDYAEKPTKAIIMLKNDRGTNYKT
                     YLEVYNELKGAYNDLWNEASMKKYGVPYDEDKLPLAYRKAIKAEIPMVLSEAEPTAFG
                     EEK"
     assembly_gap    3642..3658
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3733..4206)
                     /locus_tag="PK198_15010"
     CDS             complement(3733..4206)
                     /locus_tag="PK198_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40099.1"
                     /translation="MYKLLSKNGQTFGFLLGAVVTGLFLLIMAGGWSKFSALGDSAEK
                     YDTTIFNFGIYASIGLIIVGFVLWAFFSIFQMASNPKAAVRGLLGGVALIALFVISYL
                     TASSEVTGPLAKSVENMGVGPTGLKLISGGITTSLILTLLAGVAILLSELRNFFK"
     gene            complement(4219..5049)
                     /locus_tag="PK198_15015"
     CDS             complement(4219..5049)
                     /locus_tag="PK198_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF40100.1"
                     /translation="MRKLLTFLFVMSLGLFAANAVYAQDANNGQPATEETAQPAAEET
                     AQPAEEAEVEPVAADAPVEEVKGFQLLKKYFIDGDPGFMSLVLIALILGLAFCIERII
                     TLNIATTNTEKLLSRIDENLKSGNVDGAMEVCKSTQGPTASILYEGLKNAKQGPDAVE
                     KAIVSYGSVQMGLLEKGLVWISLFIALAPMLGFLGTVIGMIQAFDKIEAVGDISPTVV
                     AGGIKVALLTTVFGLVVAIILQLFYNYIVSKIDGIVNKMEDASIALVDIMAANNIFKK
                     "
     gene            complement(5112..5198)
                     /locus_tag="PK198_15020"
     tRNA            complement(5112..5198)
                     /locus_tag="PK198_15020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5162..5164),aa:Ser,seq:gga)
     gene            complement(5410..6204)
                     /locus_tag="PK198_15025"
     CDS             complement(5410..6204)
                     /locus_tag="PK198_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF40101.1"
                     /translation="MTFIDTHAHLYLQQFDNDRDAMLDRAMTAGCEAFFLPNIDSTTI
                     ESMLALEATRPDVCFAMMGLHPSHVKENFREELAVCEEWLSRRRFAAVGEIGIDLYWD
                     KTHLQEQLEALHIQMSWAKKLDLPIVLHTREAMDIVIEAVAEAYDERLRGVFHCFNGN
                     VEQAQRIVEMGFHLGIGGVLTYKNGGLEPVIETLGLDHVVLETDAPYLAPIPMRGKRN
                     EPVYINHVIDRLAILTGLSPEAVGETTTANARRLFAEAFVSVPAMG"
     assembly_gap    6449..6474
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6475..6671
                     /locus_tag="PK198_15030"
     CDS             <6475..6671
                     /locus_tag="PK198_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRF40102.1"
                     /translation="KQAVMLQLEDELRRLHQGLAVRLRKHLRRMPEISLFLDDTLDEM
                     YRIDSMFDQLYKDNQMGSEE"
     gene            6889..>8379
                     /locus_tag="PK198_15035"
     CDS             6889..>8379
                     /locus_tag="PK198_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRF40103.1"
                     /translation="MQTEEKYVYVFGGGTADGNASMKNLLGGKGANLAEMCLLGIPVP
                     AGFTITTEACTIYTEKGRDFVRQLITKEVKQGVVFLEKQMGKRFNDPADPLLLSVRSG
                     ARASMPGMMDTILNLGLNDEAVKGLALKSGNERFAWDSYRRFVQMYGDVVMGLKPESK
                     QEEDPFEVIIHHIKKERGVELDTDLTADDLKELVRRFKALILEKLGREFPDDPWEQLW
                     GSVMAVFKSWTNDRAEVYRSLHGIPESWGTAVNVQAMVFGNMGDNSATGVAFTRDAGT
                     GEDLFNGEFLVNAQGEDVVAGIRTPQQITLEGSRRWAVLAKVSEEDRKAKYPSLEELM
                     PDAFRLLMEAETTLENHYSDMQDLEFTIQDGKLWMLQTRNGKRTGAAAVKIAMDMLRQ
                     GMIDERTAVLRVDPVRLDDLLHPVFDLKALKAGKVIARGLPASPGAATGQIVFFADEA
                     EEWAHQGKAVILVRTETSPEDLRGMNAAKGILTARGGMTSHAAVVAR"
BASE COUNT         2070 a         2223 c         2031 g         2012 t
ORIGIN      
        1 gttccaacct gaaacctgta cctacgccat atccttcaaa ttctttacaa aaccgttcac
       61 tccgccggct ttcatcttat cggcaagcgc cttggcatca ttaatatttt tcagtgcgcc
      121 aatgacaacc cgatatgccg tctttccttg caattcgctg atgtgtacga tggccggctg
      181 accgaaaagg cttttcatgc gctcggcctg aatcagtacg ttgccataat cgtaaaacac
      241 gccgatttga acaccccaac cttcaggcgc ctgcttttgc acatcaaaac ggaacagccc
      301 cttgccggaa cctaccactg cgggtacttc agtcaccggc ttagcacttt ctttcctgcg
      361 cagcgcttcg gccacagccc ggtcggcatt cacgcggccg tagccgtatt tgagcgaatg
      421 gccattgaca taatccgaag gcgaaccgat tttatccgcc gtattttgca ggatttcttt
      481 tacttctttg gccgtcaggt caggattggc cgacaacatc agggcacaca cccccgccac
      541 caaaggcgta gaggcagagg tgccgccaaa gtgtttgtaa tactggccac gagctttgcc
      601 gtcgcgccag tatttgtaat tgcccgattc accgtccacg ccggggtccc accaggcgcg
      661 ggcactgatg atgggccagt ctccattcga cggcgcgcac accgatacct gccggccccg
      721 gttggagtag ggcgcatgtt cgtctttgct ggtgctggca gcaacggcaa tcacgtcggg
      781 atgagccgca taaaagctca caaaatcgta gccttcattg cccaccgcaa aaagaacgac
      841 gcaacctttg ccgttccgcc cctgccgggc tgccttggct atggcatttt ctttatcggt
      901 accaagtgca aaggccgcat cggtggcgcc ccaactgcaa ctgatgatgt cggcgccgtt
      961 cgctacgcaa taatcaaaca tttgctcggt ggcgcgtacc gcataagagg tgccgctcac
     1021 aggcatgaat ttcgcgttgg gagccacgcc aactaccccc tgaccattgg aggcagcgat
     1081 ggcaatagcg gcgcagggtg tgccgtgcga atatcggagg tcgccctgaa tgagttgtga
     1141 agattgattc cagaggtcga agggcttaaa aatcttgtcc ttcaaatcgg ggtgagaggt
     1201 gtcgaaacca ttgtcaatga cagccaccac cacgctcgat gcgcccaaac catctaagcg
     1261 tttccaggca tccatcactt tggcgtcggc gcctttgcga atggggtagt tggtatcggg
     1321 cagcaggcca gaattgctca ggtgccattg ctgccccagc agattatcgg gcggagcagc
     1381 gaaggcatat tcgtctaaaa ccgtgtcaag atcgggttcg gccaacttta ccattgagat
     1441 atgttgcaag gcggcagcca ctttcagcgg gttcggcgac tgagcagtta cgcgcacggc
     1501 aatgcggtcg ggcgcgcgcc gctctgtcag ttccagatga aattcctcca atacaatggc
     1561 ctgttcaggc tcgctgacgc ccggctggaa agtgataaaa atttcgccgg ttgcgatcaa
     1621 ggggcggttg ctgccttccg cgtaatagac atgcgccccg gtttccacct catccaagcg
     1681 acgtacctcg tccaatttgt cgtctacact ttcgccttcg gttttgaggg tcaccacgct
     1741 gaatccgcca agatttttgt aaacttcttc atctacaaac tcctgttcgc cggcctcgcc
     1801 ggtagttttt acacctacca acgtggaact cttgtgcaag ttaagttcgg ccttgccgct
     1861 tttgatggtt agtttgctca tttttttatg attgaaaaaa atctaacaat tttaccacgg
     1921 ctcttcccct gtggctaata cgcccctttt ccttgttgct catttgtgca aaactgcgcg
     1981 tttcaccttc gggaacaaac accggatcat acccaaagcc gccgtctccc gcaggcgttt
     2041 cggcaatgcg cccctccaca atgccttcaa acaaatattc cctgccttcc aaaatcaatg
     2101 ctatgacggt gcgaaaacgg gcgctgcggt cgctgtgtgc ggcgagttta tccaaaagct
     2161 tacgggtatt ggcagcaggg tctttgtcat cgccggcata acgggcagaa tagacgccgg
     2221 gttcgccatg cagggcatat acttccaggc cggtgtcttc tgcaaaacaa ttaactccga
     2281 aatgctcata cacataccga gccttttgca gcgcattgcc gggaatggtg tcggaagtct
     2341 ccggcaagtc ggtcgggcag ccaatttccg gcaggccgat cagccggatg ccttcgggca
     2401 acaactcaga tacttcactg attttgtggg cattgccggt ggcgaaaaca atttccatgt
     2461 attctctttt cagcaaaagt aaggaaatag ggctaaaaca agatgagacg atattcaaaa
     2521 atccaataaa tgcaacaagc cggccctgca atgcaagaac cggcttgtct ttttttatct
     2581 ggtatgccga agcaacaccc ttgactaatc agtcatcata aagtagcatc cggacgacgc
     2641 ttggccgagt agttcacctt gagctggcct gatttgcgca atgccgtttt tacatctgcc
     2701 acgataccca tggttacatt gccatccacc cttagagaag tcgtaatttg agggcgacga
     2761 ctctccggta ctttggttct aaagctttcc atgaagtagc ggatgtctgc ctcttcctgg
     2821 cctacctttg caaacttgtc gttgagctga atttgcggct tggtgccgta aatcggtcgg
     2881 tatttttcga tagggcgacc aacgtaaatg gtagctacta aagatttttc ctccaatttc
     2941 gtcaattcag acgcgaaagg tgtgatgata gtcaatttca actctgaatc ccgcatcttg
     3001 gttactacca tgaagaagaa caacagcatg aaaatgatat ctggcagcga agcggtagag
     3061 atgttcggat cggccttgcc tcttttttta gcaaattttg acatgatctc ttgtttttgc
     3121 tctgccggag gggcaggtca ttatctgatt tacttttctt cgccgaatgc ggtaggttct
     3181 gcttccgaaa gcaccatagg aatctcagcc ttgatggcct tgcggtaagc caacggaagt
     3241 ttatcttcgt catacggaac gccgtatttt ttcatactgg cctcattcca aaggtcgtta
     3301 tacgcgcctt tcagctcgtt gtaaacctcc aaataggtct tgtaattcgt accccggtca
     3361 tttttcaaca taatgatcgc cttggtaggc ttctccgcgt aatcttcgcg tccatacgga
     3421 ttggaaataa attcgaccgc attcttgcga agatcacgaa tactcattgg ttgattgcgc
     3481 accagcaact gattttgggc attaaccaaa acagagtaaa cgttgcgcgt tttaagctgg
     3541 gtaatgtcgg gttcttcttc cgaccacggc ggcagtttca ccgtgatgcc cttgtcttcc
     3601 acgatggtag tggtcacaag gaagaagatg agcaacagaa annnnnnnnn nnnnnnnnat
     3661 gtctgccata gagctggcat tgatttcatt atttgcccgt cctttcgatt ttgacttatt
     3721 ggccatgaaa cattatttaa agaagttcct caattcagac agcaatatcg caacgcctgc
     3781 gagcaaagtc aaaatcagac tggtagtgat tccgccactg atcagcttga ggcccgtagg
     3841 acctacaccc atgttttcca cagactttgc aagaggccct gtcacttcgc tactcgcggt
     3901 caagtatgaa ataacaaaaa gtgctatcaa ggctacaccg cccagtaaac cgcgtactgc
     3961 tgctttgggg ttggaagcca tttgaaaaat tgaaaagaag gcccaaagga caaagccaac
     4021 gataatcaga ccgatggatg cgtagatgcc gaaattgaaa atagtggtat catacttttc
     4081 ggcggaatcc cccaatgcgg agaatttact ccaaccgccc gccattatga gcaggaatag
     4141 gccggtgact acggctccta atagaaagcc gaacgtctgt ccgtttttgg atagtaattt
     4201 atacattgga tggatggatt actttttgaa aatgttgttc gcagccatga tgtccaccaa
     4261 tgcgatggag gcatcttcca ttttattgac gataccgtca attttggaaa cgatgtagtt
     4321 gtagaacaac tgaagaatga tggctaccac aagaccgaat accgtggtca aaagagccac
     4381 tttaataccg ccggccacca ccgtaggaga aatatcgcct actgcttcga ttttgtcgaa
     4441 ggcctggatc ataccgatta ccgtacccaa gaacccaagc atgggcgcca aagcgatgaa
     4501 cagagaaatc caaaccagac ctttttccaa caagcccatt tgaacgctac cataagaaac
     4561 aatagctttt tccactgcat caggcccttg cttggcattt ttcaagcctt catacagaat
     4621 gctggcggta ggaccttgcg tggacttgca tacttccata gcgccgtcta cattgcctga
     4681 ttttaagttt tcatcaatac gggagagcaa cttttcggta ttggtcgtag caatattgag
     4741 cgtgatgatg cgctcaatac agaaagccaa gccgaggata agagcgatca aaacgaggct
     4801 catgaagcca gggtcgccat caatgaagta ctttttgagc aattggaaac cctttacttc
     4861 ttctaccgga gcatcagcag caaccggctc gacttctgct tcttctgccg gttgagcggt
     4921 ttcttctgct gctggctgag cagtttcttc agtagcaggt tgtccattgt tggcatcttg
     4981 agcgtacact gcattggcag cgaaaaggcc caaagacatt acgaacagga aggtcaataa
     5041 tttgcgcata gttgacgtag ttttgcctga aatttaaatg attaagttga ttgtttcgtt
     5101 tgtcaaaaca gcggagagag cgggattcga acccgcgata cccttccggg tatacacgct
     5161 ttccaggcgt gcgccttcga ccgctcggcc acctctcctt cattagcagt gggcgaaaac
     5221 agactttagt tttcattttc accccttgcc ctcttgcctg ggaaaatctt acaaattcct
     5281 cagtactgcg ttcagtactt ctgcaaatcg gagcaaaaat aattattcac tcattaccac
     5341 caactccgac gctgcaaaaa ttgcaaaaag ttatcacaaa aaataaaaat tttcctgccg
     5401 gacgacattt cagcccattg caggcaccga aacgaatgcc tcagcgaaca aacggcgggc
     5461 gtttgcagta gtcgtttcgc ccacggcctc cgggctaaga ccagtcaata ttgccagccg
     5521 gtcaatgaca tgattaatgt acacgggttc gttgcgcttg cccctcatcg gtatgggcgc
     5581 gaggtacggc gcatcggttt ccaaaacaac atgatccagc cccagcgtct caatgacagg
     5641 ttccaaaccg ccgtttttgt aggtcagcac cccgccgatg ccgaggtgaa aacccatttc
     5701 tacaatgcgc tgcgcctgct ctacattgcc gttgaagcag tgaaatactc cccgcagccg
     5761 ttcatcatac gcctccgcaa cggcttctat cacaatgtcc atcgcctcgc gggtgtgcag
     5821 cacaatgggc agatcgagtt tcttggccca gctcatttga atatgcaggg cctccaattg
     5881 ctcttgaaga tgggttttgt cccaatacaa atctataccg atctcgccga ccgcagcaaa
     5941 acggcggcga ctgagccatt cttcacacac ggccaattct tcccggaagt tttctttcac
     6001 atggctgggg tgcaggccca tcatagcgaa acaaacatcg gggcgggtgg cttccagtgc
     6061 caacatggat tcaatcgtcg ttgaatcaat gttgggcaga aaaaaagcct cgcagccggc
     6121 agtcatggca cgatcaagca tggcatcccg atcgttgtcg aactgctgta aataaagatg
     6181 tgcgtgcgta tctatgaatg tcatgatgcc acaaatgtag gtatttgatc tgaatatgcc
     6241 ttgttatcct ttctttaaaa aaaagtacct ttgccaaaaa ttgcagcaca tggaatcgaa
     6301 gcgacagctc caagtagccg aaatggtgaa gcgccacttc agcatcgtca tgatggagga
     6361 aggcacctat atatatggta gcggcatcat ggtgaccgtt acctccgtaa aaatgactcc
     6421 cgacctctcg atggcaaaaa tttacctcnn nnnnnnnnnn nnnnnnnnnn nnnnacaaac
     6481 aggccgtgat gctgcaattg gaagacgaat tgcgcaggtt gcaccaaggc ctggcggttc
     6541 ggctgcgcaa acatctgcgc cgcatgccgg aaatctctct tttcctcgac gacacgctcg
     6601 acgaaatgta tcgcatagac tctatgttcg atcagttgta caaggataat caaatgggga
     6661 gcgaggagta ggcggaaagc agtaggcgga aagcagtagg cagtgggcgg taggcggttc
     6721 caacccgaaa ccttgaaccc gaaacccgaa actcgcccgt tccaccccga atcattcctc
     6781 actgacaata atcacccctg catctgacat acatcatttc cagtaaggtt gcatacatcg
     6841 tacttcgtgc ttgtttttca agcatttatc aacgaaaaaa agtacgacat gcaaacggag
     6901 gaaaaatatg tttacgtctt cggcggcggt actgccgacg gcaatgcaag tatgaaaaac
     6961 ttgctcggcg gaaaaggagc caatctggcc gaaatgtgcc tgctgggcat tcctgttccg
     7021 gcgggcttca ccatcacaac ggaagcctgc accatttata ccgaaaaagg ccgcgacttt
     7081 gtgcgccaac tcatcacgaa ggaagtgaaa caaggcgttg tttttttgga aaaacaaatg
     7141 ggcaaacgat tcaacgaccc tgccgacccg ctcctgcttt ctgtacgctc cggcgcacgc
     7201 gcttccatgc caggcatgat ggacaccatc ctcaatctcg gtctgaatga cgaggcggtg
     7261 aaaggattgg cgctcaagtc gggcaacgaa cgcttcgctt gggactccta ccgccgtttt
     7321 gtacaaatgt acggcgatgt ggtgatgggc ctcaaacccg aatcaaaaca agaagaagac
     7381 ccgtttgaag tcattattca ccacatcaaa aaagagcgcg gcgtagaatt ggatacagat
     7441 ttgactgccg acgatctcaa agaattggtg cgccggttca aagcgctcat ccttgaaaaa
     7501 ttgggcaggg aatttcccga cgatccctgg gaacaactct ggggttccgt catggcggtt
     7561 ttcaaaagct ggaccaacga ccgcgccgag gtatatcgca gtctgcacgg cattcccgaa
     7621 agctggggca ccgccgtcaa cgttcaggct atggtattcg gcaacatggg cgacaacagc
     7681 gctacgggcg tggccttcac ccgcgatgcc ggcactggcg aagacctttt caacggtgag
     7741 tttttggtga atgcacaggg cgaagacgtg gtggcaggca tccgcacgcc gcaacagatc
     7801 acgctcgaag gctcgcgccg ctgggctgtg ttggccaaag tgtctgaaga agaccgcaaa
     7861 gccaaatatc cttctttgga agaactgatg cctgatgcgt tccgcttgct catggaggcc
     7921 gaaacaacgc tcgaaaatca ctacagcgat atgcaggatt tggagttcac catccaagac
     7981 ggcaaactct ggatgctcca aacccgcaac ggcaaacgca ccggcgcggc tgccgtcaaa
     8041 attgccatgg acatgctgcg ccagggtatg attgacgagc gcaccgccgt actgcgcgtg
     8101 gatccggtgc gtttggacga cctcctgcac cccgtattcg atctcaaagc gctcaaagcc
     8161 ggcaaggtca tcgctcgcgg tttgcctgca tctcccggcg ccgctaccgg acagatcgtg
     8221 ttctttgccg acgaggccga agaatgggcg catcagggca aagccgtcat tttggtacgc
     8281 accgaaacat cgcccgaaga cctgcgcggt atgaacgccg ccaaaggcat cctcaccgct
     8341 cgcggcggta tgacctcgca cgctgccgta gtggcgcgc
//
LOCUS       DAORLF010000195         8365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4604_length_8365_cov_2.187726, whole genome shotgun sequence.
ACCESSION   DAORLF010000195 DAORLF010000000
VERSION     DAORLF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_8365_cov_2.187726"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1354
                     /locus_tag="PK198_15040"
     CDS             80..1354
                     /locus_tag="PK198_15040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRF40104.1"
                     /translation="MAAKHHQIVIVGGGTAGITVAAQLLRADKKLDVAIIDPTKKHYY
                     QPAWTLVGAGVYDFKATERDEASVIPKNVTWIQQPAQDLQPDDNKVVLGNGDEVTYDY
                     LVLCPGIQIDLNGIEGLTETLGKNDVCSNYTDPKYTWEVLQNFKGGTAIYTQPATPIK
                     CGGAPQKIMYLADDYYQKKGVKDKTKVSFITPGSVIFGVPEFAKTLMEVVNRKGIELR
                     FFHKLTKIDGPNKMAYYSIVGGDGDPAKLYHNKLDLGDQVISPTEVAVPFDMLHLAPP
                     QSAPDFVKRSKLAHQEGPDKGWINVNINTMQHNVYPNVFGLGDAAAVPTARTGAAVRK
                     QAPVLVGNLVHLIQNKGAAMNPGYQGYSSCPLVTGYGKMVLAEFKYNNVRDSDKLIST
                     FVDTTKEQYSMWLLKMYGLPFIYWKMMLKGLM"
     gene            1847..2224
                     /locus_tag="PK198_15045"
     CDS             1847..2224
                     /locus_tag="PK198_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40105.1"
                     /translation="MKKLLNAALILTSLIGYLEWGGGNHQFLFQIEYELIFGALKDLN
                     NFKHPFVLLPMLGQVLILITLFQKTPGRTLTFIGLACMSLLMLFILLVGVLGLNLRVI
                     LSTIPFVITGILVLRAHWKRAES"
     gene            2534..>2898
                     /locus_tag="PK198_15050"
     CDS             2534..>2898
                     /locus_tag="PK198_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRF40106.1"
                     /translation="MKHQVVIITGVAGIGKSSVARLLAQKLGCSYYDGDDFHPQANVE
                     KMQRGTALKDEDRAPWLQSIHNFIVARLPLESLVLACSALKQKYRDVLEQGLPAAQVQ
                     WVHLQGDFDLVYNRILQRAG"
     assembly_gap    2899..3037
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3038..>3440
                     /locus_tag="PK198_15055"
     CDS             <3038..>3440
                     /locus_tag="PK198_15055"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF40107.1"
                     /translation="LEAQSEMGVLGLGVMGTNLARNIADKGIALSVFNRHVEGAEENV
                     ALKATQKYPELAPARPYDDLRTFVASLQKPRKILLMINAGPAIDEVLRQLTPLLSEGD
                     VVIDGGNSHYRDTGRRMDDLSRHRIHYLGAGV"
     assembly_gap    3441..3462
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3463..>4373
                     /locus_tag="PK198_15060"
     CDS             <3463..>4373
                     /locus_tag="PK198_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40108.1"
                     /translation="LLGPAIMPGGSAEAYALAAPVLTRIAAVNAAGERCCAYIGAGGS
                     GHFVKMVHNGIEYAEMQLIAETYAHLRWEQGLSPEGIAEIFAEWNAGSEQSYLLEITI
                     VVLLKKDTDGLPLLDKVSDAAANKGTGGWTSIAACDLGVPIPSITAALFARYQSALKT
                     LRTEYAAQFQPETAGRKPALDDLKNLYRFCRLMNHHQGFRLIRAASEAYQWDIGLEAL
                     AGIWSGGCIIRSRLLADIRAHLAHQPDILLVPSVAGFLAGHYTSVARAYQMLAGGREA
                     IPCISAGLEYFKYMTQEESSASVLQAQ"
     assembly_gap    4374..4460
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4704..4731
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4771..>5057
                     /locus_tag="PK198_15065"
     CDS             4771..>5057
                     /locus_tag="PK198_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HRF40109.1"
                     /translation="MKIQLLLSVLGGMSLLLLLIIRLRLPAFIALLIAAIVTALMAGM
                     ETGQIIETVQKGMGSTLGFVATVVGLGAIFGGILEKTGGAEKIATSLLNFFG"
     assembly_gap    5058..5067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5068..6107
                     /locus_tag="PK198_15070"
     CDS             <5068..6107
                     /locus_tag="PK198_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HRF40110.1"
                     /translation="KKAPLALLLTGFMVGIPVFFDVGFIILFPMLPAVHQKTKKSLVF
                     YAIPLLAGLAVTHAFIPPTPGPIAVADILGANLGMVIVVGVLAGLPAAYTSGLLYGKY
                     IGNKIDIQIPHAPEPVARKSELHTGLVFAILLLPIALILMSTVAKTVYKNENAVTAAI
                     QLLGHPFSALILANILAWYVLGRNYGFSTRQLTEISSKSMQPAGVIILVTGAGGVLKQ
                     VLVDTGAGKMIAEAMQDAGLSIIVFSFLAATIVRVLQGSATVAMITAAGLVAPLLASY
                     QLNAVEVAALVTSIASGATMLSHLNDSGFWLVKEFLHLSEKQGLQTWTIASSILGLTG
                     FVMSCLIFWVG"
     gene            complement(6131..>6949)
                     /locus_tag="PK198_15075"
     CDS             complement(6131..>6949)
                     /locus_tag="PK198_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40111.1"
                     /translation="ALLQTMTAVNDWADLNQVPVSMGEFGVATSADADSRCNWINAVM
                     NAVNTYGFSYFYWDAISPSDAFGFFNSGVVSEATVIPCFRNAMGLYMAPLAIELLNFD
                     LRCQPGRSTLQWTTMSDELNARFELQRSTDARTWHSIHAGSTVSGRQSYSFTDEAATD
                     GALYRLRSISADGSERFSPVRIATCGDLPELKLWPNPARDEARLQLSGDGDGILRWSL
                     WDMQGRLLMQSEQYAGSGRQEAIISPEGLPAGMYNLSIQTAKGRTAHRRWVLTR"
     assembly_gap    6950..7042
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7043..8230)
                     /locus_tag="PK198_15080"
     CDS             complement(<7043..8230)
                     /locus_tag="PK198_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRF40112.1"
                     /translation="MHKNIYTILFLCASMTTLLSQPIIYNDNSTLITGTWHASSGGGG
                     TSSLAEVTGQTPHEGARHYRFDYNFSNYWAGIGLNMDNWGGGPARDFSAYTHLRIAYR
                     GLSAGQNLIIQLRNGGNFGNTATIGPATANYTVVELSLFSLTAGTNVSAAAVRELNIS
                     IESTAQSGSGTAYVDAIELVNGSSGGGMPASATTMARAASMTAGVNTSNWLEAYWLLP
                     FNAYPEVNRYTRARVQALRDAGFTTFRLPVTFERLTQPGNSSQLNTNQVAFSLVDSMI
                     LWAAQMDFKLIICNHHGLSLTNSNYQTELPRLQAIWTQLAQRYGHLHPDRYYFEVYNE
                     PDNNITNANWRTVATALLQTLRTHESVLHSVFVGAANWNSAGALISFTPLDDADIIYT
                     FHYY"
BASE COUNT         1824 a         2097 c         2227 g         1838 t
ORIGIN      
        1 gtcacccctt cactaagcat agaaaactca atgaacaatg tgattattca gccaatgtct
       61 caccaaaaaa acaccaacga tggcagccaa acaccaccaa atcgtaatcg tcggcggcgg
      121 caccgcaggc atcaccgtag ccgctcagtt gttgcgcgcc gataaaaaat tggacgtggc
      181 aatcattgac ccgactaaaa aacattatta ccagcccgcc tggactttgg taggcgcggg
      241 ggtatatgac ttcaaagcca ccgagcgcga cgaggcatcg gtcattccaa aaaatgtgac
      301 ctggattcag cagcccgctc aggatttgca acccgacgac aacaaagtag tactcggcaa
      361 cggcgatgaa gtgacgtatg actacctcgt gctttgcccc ggcatccaga ttgacctcaa
      421 cggcattgag ggcctgacgg aaacgctggg caaaaacgac gtgtgcagca attacactga
      481 tcctaaatac acctgggagg tactccagaa tttcaaaggc ggcacggcca tttataccca
      541 accggccacg cccatcaaat gcggcggcgc ccctcagaaa atcatgtatc tggccgatga
      601 ctactaccag aaaaaaggcg tgaaagacaa aacgaaagta tcgttcatca cacccggttc
      661 ggttattttc ggcgtgccgg aatttgccaa aaccctcatg gaagtggtca atcgcaaagg
      721 catcgaactg cgctttttcc acaaactcac caagattgac ggccccaaca aaatggccta
      781 ttacagcatc gtgggcggcg acggcgaccc ggccaaattg taccataaca agttggacct
      841 cggcgatcag gtcatctctc ccaccgaagt ggccgtacct tttgatatgc tgcacttggc
      901 gccgcctcaa agcgctcccg acttcgtaaa acgctccaaa ttggcgcatc aggaaggccc
      961 cgacaaaggc tggatcaacg tcaacatcaa taccatgcag cacaatgtgt atcccaatgt
     1021 cttcggcttg ggcgatgctg ccgccgtgcc caccgcccgt accggcgccg ccgtgcgcaa
     1081 acaggcgcct gtgttggtcg gcaatctggt gcatctgatt caaaacaaag gcgcggccat
     1141 gaatccaggt tatcagggct actcctcctg cccactcgtt acggggtatg gcaaaatggt
     1201 gctggcggag tttaaataca acaacgtacg cgacagcgac aagctcattt ccactttcgt
     1261 ggataccacc aaagagcaat acagcatgtg gctcctcaaa atgtatgggc tgcccttcat
     1321 ctactggaaa atgatgctga aaggcttgat gtaaacatct gacaaacagc gaataaaata
     1381 acagataatc cgactgccgg ggtgtgtaca ttccggcagt tttttttatc cctgcgcgag
     1441 gtgacatcta accgcgccct aagcgcggga aggtgtcatc tcgcggctgc gaagggaaaa
     1501 atgcccattg aaatattgat tttttttcgg ccatataccc cagagtcctg atgatacttc
     1561 aagtctaaaa tgatgagaag ttattcaagg tttatattaa caatgggcta tcgaaagaga
     1621 aattgagagt agcccttttg acaatgggca tttcgatatt ttttacaggc attactggtg
     1681 aaaaagtcta tgtatgctcc aactgtagtc atgcagaggt tgaataagac actcgcccca
     1741 tttatcacaa tcttgtcttg ttaaattgta cctcagagaa gcctcaatat gtattggaga
     1801 gcaagtgttt taattacctt tgtcatacat tacaaaacct gtcaccatga aaaaattact
     1861 caatgcagcc ctcatcctca catctctgat cggttatctg gaatggggcg gcggcaacca
     1921 ccaatttctg ttccaaatcg aatacgaact gattttcggg gcgctcaaag acctcaacaa
     1981 tttcaagcat ccattcgtct tgctgcccat gctggggcag gtgctgattc tcattaccct
     2041 gtttcaaaaa acgccgggca gaacgctcac cttcatcggt ctggcctgca tgagcctgct
     2101 gatgttgttc attttgttag tcggcgtttt gggactcaat ctacgcgtga tactctccac
     2161 cattcctttt gtcatcaccg gcattttggt gctgcgcgcc cattggaagc gggctgaaag
     2221 ctaaggggcg ataaaaaaat aaagtgatca tttacaaaat tcgggatgat tcatgtttcg
     2281 ctccgccatc cggtgaatcc tgtctaaaga ataagcgagg tacagcttca gacgcagaaa
     2341 gacgcagatt tttatgatcg ttatgtcctc tacgagcagc gcaaatcata caaatcataa
     2401 atagatcagc gtcaaaaata ggacgcggtt catatttcag aatttcagca catgctttgt
     2461 tctgtccaat gtgttataca cgccactcgt cggcttgcgt ttcgcttttg ggaatagttt
     2521 ttacttttat cggatgaagc accaagttgt cattatcaca ggcgtggccg gcatcgggaa
     2581 atccagcgtg gcccgattgc tggcacaaaa gctgggctgt tcttactacg acggcgatga
     2641 ttttcaccca caggccaacg tggaaaaaat gcagcgcgga acagccctga aagacgaaga
     2701 ccgcgcaccc tggctgcaat ccatccacaa ttttatcgtc gcccgcctcc cattggagtc
     2761 cttggtgctg gcctgctccg ctttgaaaca aaaatacaga gacgtgttgg agcagggtct
     2821 gccggcggct caggtgcagt gggttcattt acagggcgat tttgaccttg tttacaatag
     2881 aatcctgcaa agggccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc tggaagccca atcggaaatg
     3061 ggcgtcctgg gcttgggtgt gatgggaacc aatctggccc gaaacatagc agacaaaggc
     3121 attgcgctct ctgtttttaa tcggcatgta gaaggcgcag aggaaaacgt agccctgaaa
     3181 gccacacaaa aataccccga actggccccg gcccggccct acgacgacct gcggaccttt
     3241 gtggcctctt tgcaaaaacc gaggaaaata ctcctgatga tcaacgccgg ccctgccata
     3301 gacgaagttc ttcgacaatt gacgccgctt ctttcggagg gagatgtcgt gatagacggg
     3361 ggtaattcgc actaccggga caccgggcgg cggatggacg atctgagccg acaccgcatt
     3421 cactacctcg gcgccggcgt nnnnnnnnnn nnnnnnnnnn nncgctcctg gggccggcca
     3481 tcatgccggg cggctcggcg gaggcttacg cattggcggc gcctgttctt acacgcatcg
     3541 cagccgtgaa tgcagccggc gagcgttgtt gcgcgtacat tggcgccggg ggcagcgggc
     3601 attttgtaaa aatggtacac aacggcattg aatacgccga aatgcaactg atcgccgaga
     3661 cgtatgctca tctccgctgg gaacaggggc tttcgccgga aggcatcgcg gaaatttttg
     3721 cagagtggaa tgccggttcc gagcaaagtt atctgctgga aataacgata gttgttttac
     3781 taaaaaagga taccgacggc ctccccctgc tcgataaggt gtcggatgcg gcggccaaca
     3841 aaggcaccgg cggctggacc agcatcgcgg cctgcgatct gggcgttccc atcccctcca
     3901 tcacggcggc gctgtttgcc cggtaccagt cggcactaaa aaccttgaga actgaatacg
     3961 ccgcgcagtt ccaacccgaa accgccggcc gcaaacccgc tctggatgac ttaaaaaacc
     4021 tgtaccgctt ttgccggctg atgaaccacc atcagggctt tcggctcatt cgggcagcat
     4081 ctgaggcata ccaatgggac atcggacttg aggcattggc aggtatttgg tcgggaggct
     4141 gcatcatccg gtcgcggctg ctggcggata ttcgcgctca tttagctcat caacccgaca
     4201 tcctgctcgt cccctcggtc gctggtttct tggctggtca ttacacgagc gtggcgcggg
     4261 cgtaccaaat gctggcgggc ggtcgtgagg ccataccctg catttcggcg ggcctcgaat
     4321 attttaaata catgacgcag gaggagagca gcgcctctgt gttgcaggcc cagnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn tcattaacaa acgaatagct tcgagccgcg agctacgagt
     4501 tgattttcca aaataaaaaa gtcactcatc ttcaacggtt gattttgaaa tctcccctct
     4561 gttttcatgc gacaccttcc agcctcgcta aagatcggct gttagatgtc acctgaaaac
     4621 acaagcgagg tgacatctaa cgccgggatg caacaactgg cggcggaagg tgtcatctcg
     4681 cggctaagct gcctgccggc gagnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctcgcagc
     4741 tttagggaat aatatgttaa actattgacc atgaaaatcc aactcctact atccgtgctg
     4801 ggcggcatga gcctgctgct tttgctcatc atcaggttgc gtcttcccgc attcattgcc
     4861 ctattgatcg ctgccattgt caccgcgctg atggcaggca tggagaccgg ccaaatcata
     4921 gaaaccgtgc aaaaaggcat gggcagcacc ctgggttttg tggctaccgt ggtgggactg
     4981 ggggccatat tcggcggtat tttggaaaaa accggcggcg ctgaaaaaat cgccacgagc
     5041 ctcctgaatt tttttggnnn nnnnnnnaaa aaaaagcccc actcgccctc ctgctgacgg
     5101 gttttatggt aggcattccc gtctttttcg acgtcgggtt catcattctt ttccccatgt
     5161 tgccggcggt gcatcaaaag actaaaaaat cgctcgtttt ttatgccatt cctttgctcg
     5221 ccggcctcgc tgtcacacat gcctttattc cgccgacgcc cggaccaatt gccgtggcgg
     5281 atattttggg cgccaacctc ggcatggtca tcgttgtggg cgtgctggcc ggtttgcccg
     5341 ccgcctatac gagcggcctg ctgtacggga aatacatcgg gaataaaata gacattcaaa
     5401 taccccatgc accggagcct gtcgcccgaa aatcggaact gcacacgggg cttgtttttg
     5461 ctattctgct gctgcccatt gccttgatac taatgagtac ggtggcgaaa accgtctata
     5521 aaaatgaaaa cgctgtgacg gcggccatcc aattgctggg acatccgttt tcggcgctca
     5581 ttttggccaa tatccttgcc tggtatgtat tgggtagaaa ttacggattc agcacccggc
     5641 aactgaccga aatttcgtcc aaatccatgc agccggcggg agtcattatt ttggtaacgg
     5701 gcgcgggcgg cgtgctgaaa caagtgctgg tagatacagg cgcgggcaag atgattgccg
     5761 aagcgatgca ggacgcgggc ctgagcatca ttgttttttc ttttttagcg gccaccattg
     5821 tcagggtatt gcagggttcg gccaccgttg ccatgattac cgctgccgga ttggtggcgc
     5881 cgctgctggc ctcgtaccaa ctcaacgcgg tggaggtagc cgcgctggtt acgtccatcg
     5941 cgtcgggcgc gacgatgttg tcgcatctga atgacagcgg tttctggttg gtcaaagagt
     6001 tcttgcacct cagcgaaaaa cagggcctcc aaacctggac catcgcctcc agtattctgg
     6061 gcctgacggg ctttgtgatg tcctgtttga ttttttgggt gggatagctg aggccatcag
     6121 gccggctcat ctacctcgtc agcacccacc tcctatgcgc cgtgcggccc ttggcggtct
     6181 gaatgctcag attgtacatg cctgccggca atccctccgg tgaaatgatc gcctcctgcc
     6241 ggcccgaacc tgcgtactgc tcagactgca tcaggaggcg gccctgcatg tcccacaagc
     6301 tccagcggag gatgccgtcg ccgtcgcccg aaagctgcaa gcgggcttca tcgcgcgccg
     6361 gattgggcca gagctttagt tccggcaagt cgccgcaagt ggcgatgcgc accggcgaaa
     6421 agcgctccga accatcggcg gagatgctgc gcagtcggta gagcgcgccg tcggtggcgg
     6481 cttcgtcggt gaaggagtac gactggcgac ccgataccgt gctgcctgcg tgtatgctgt
     6541 gccaggtacg ggcgtcggtg ctgcgctgga gttcgaagcg ggcgttgagt tcgtcgctca
     6601 tagtggtcca ttgcagggtg ctgcggccgg gttggcaacg cagatcgaag ttcagcagtt
     6661 cgatggccaa gggcgccatg tacaggccca tcgcattgcg gaagcaggga atgacggtgg
     6721 cctcactgac aacgccgctg ttgaaaaagc cgaatgcgtc gctgggcgaa atggcatccc
     6781 agtagaaata ggaaaatccg taggtattga cggcgttcat caccgcgttg atccaattgc
     6841 aacgactgtc ggcatcggcg gaagtggcca cgccgaactc ccccatcgag acgggtacct
     6901 gattcagatc ggcccagtcg ttgacggcag tcatggtttg cagcagcgcn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nngtagtagt ggaaggtgta gatgatgtct gcatcgtcga
     7081 ggggggtgaa ggagatgagc gcgccggcgg aattccagtt ggcggcgccc acaaagacgc
     7141 tgtgcagcac cgattcatga gtgcgcaggg tttgcagcaa tgccgtagct acggtgcgcc
     7201 agttggcgtt ggtgatgttg ttgtcgggtt cgttatagac ttcaaaataa taccggtcgg
     7261 ggtggaggtg gccgtagcgc tgggccaatt gcgtccagat ggcttgcagg cggggcagtt
     7321 ccgtctgata attgctgttg gtgagcgaca gtccgtggtg attgcaaata atgagtttga
     7381 aatccatctg agcggcccat aggatcatgc tgtccacgag gctgaaagcg acctgattgg
     7441 tattaagttg cgaactgttg cccggctggg tgaggcgttc aaaagtgacg ggtagccgga
     7501 aggtggtgaa gccggcatcg cggagggcct gcacgcgggc gcgggtgtag cggttgacct
     7561 ccgggtaggc gttgaagggc agcagccagt aggcttcgag ccagttggag gtattcacgc
     7621 cggcggtcat agaggcagcc ctggccatag tagtagccga tgcgggcata ccgccgcccg
     7681 aactgccgtt gacgagttcg atagcatcca cataggccgt gccggaaccg ctttgcgcgg
     7741 tagattctat gctgatgttg agttcgcgca cggcggcggc agatacgttg gtacccgccg
     7801 tgagggaaaa caaagacaac tcaacgacgg tgtaattggc cgttgccgga ccgatggtgg
     7861 cggtgttgcc gaaattgccg ccattgcgca actgtatgat gaggttttgg cctgccgaca
     7921 gcccccggta ggcgatgcgc aggtgggtgt aggccgagaa atcgcgggcc gggccgccgc
     7981 cccagttgtc catattgaga ccaatgcctg cccagtagtt ggaaaaattg tagtcgaagc
     8041 ggtaatgccg ggcgccctcg tgcggggttt ggccggtcac ttctgcgagg ctcgatgtgc
     8101 cgccgccgcc gctggatgcg tgccaggtgc cggtgatgag ggtgctgttg tcgttgtaga
     8161 taatgggttg ggagagcagc gtggtcatgg acgcgcagag gaaaaggatg gtgtaaatgt
     8221 ttttgtgcat tttggatttt ggatttcgga ttcctgattt cggatttctg atttcggatt
     8281 cctgatttcg gattcctgat ttcggattcc tgatttcgga ttcctgattt cggattcctg
     8341 atttcggatt cctgatttcg gattc
//
LOCUS       DAORLF010000196         8360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4610_length_8360_cov_1.827213, whole genome shotgun sequence.
ACCESSION   DAORLF010000196 DAORLF010000000
VERSION     DAORLF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_8360_cov_1.827213"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1405)
                     /locus_tag="PK198_15085"
     CDS             complement(<1..>1405)
                     /locus_tag="PK198_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40113.1"
                     /translation="DCPNPVATPGATITYNFQVDVPNCPVGASATIRVVIPPVVNMSP
                     NRTVCIGTPVQLNLAADNESLYRWTSSTDPNFTSNDPLLIAAPLVTTTYTLRASKAGC
                     PDAVYTVTITVIQPPTADAGPDQTICQGSTATLAGTFGGAATSGIWTTASGGSFSPNA
                     MTANAVYTPTTTGIITLRFTATDPNNVCPQATDEMALVVNPAATVNAGPDQAVCSGNP
                     VTLAGTIGGGATSAVWTASVPGGVFNPNANTLNATYTAPAGVTSVTLTLRTNDPDGPC
                     PAAEDQMVIAVTPPATVNAGQDFSVCRPVGFNLTAAGTAVGGVQSYSWSGPGITGSNN
                     TASVSVSSSAALSSPAMYIVNYSYGPNANLICATVRDTILVTIEESVRIDALVSSRGD
                     SSVVIGSAVTITANTTPGDPVGAMYLWSVNGGAEQMGGPAFTDTPIPATGVTSVNYRL
                     IVKSAAGCLDTAFLDINL"
     assembly_gap    1406..1522
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1523..>3299)
                     /locus_tag="PK198_15090"
     CDS             complement(<1523..>3299)
                     /locus_tag="PK198_15090"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40114.1"
                     /translation="FTQEQVCETPGVVINAVTNTQPIRSSWTGGSTRTGLADINPDTG
                     APVLDDFDCGSPATPGAGGDRFVRTIPALEGQVFVILTNDFGNQIGEEGISVDWSTSD
                     PDVLAPPPPVVTGNDTTICIGASVQLSVISAIGNISWSDPTGTLSCTNCPNPVATPSA
                     TTTYTALIDAVCYDETIDVKVTVFDVDAGPNLTVCRGEQIQIVSGTDYENATYEWTAP
                     AGVTFSCTDCPDPMVEGSVSGEYSVRVRLITPNCILSDSLRFNVLPQPAPQFAISDNV
                     EICVGASVNLGGAATPDVTYSWSSVPGGFASGEANPQVSPTQTTTYYLSATNSLCPLS
                     SLDSVTVTVYQTPSLTIPADTAVCQGQPILLTNTTAEQDVTYQWSGPDDIENPDAATT
                     VAQPQNSGDYVLRATRGVCEVSDTFTVTITPIDLAILNPDTLRICLGETVDLQLNITP
                     SNAVASWTPAIWLSSNTGNSIQATPETTITYFATVNVPGCSKTDRIVIEVDSLPADRA
                     IIADPAKDPYCPGDIVILRSTNYEPSDFPDIEFQWLPGPGFETPDTLWNMVISAIDTA
                     TYRRVIIHRGCVDTVSITINVVEPPM"
     assembly_gap    3300..3334
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3335..>3671)
                     /locus_tag="PK198_15095"
     CDS             complement(<3335..>3671)
                     /locus_tag="PK198_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40115.1"
                     /translation="DLREPGIYYIVVADGNGCTPYNISITPTECELPAGLVNALCNPL
                     NGCIEEGGVPSVFNFQDGFQDIDLIEDVNSGCFPTNGVGAEADFYWFTIQAQADGPFG
                     FILEGAGAAS"
     assembly_gap    3672..3711
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3712..4858)
                     /locus_tag="PK198_15100"
     CDS             complement(<3712..4858)
                     /locus_tag="PK198_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HRF40116.1"
                     /translation="MKFFYAFLVFIAFSGFVTAQAPINDNCNGLIDLGVAPICPQTVY
                     TNVNATATNIGFGNNPSCFNGGTAQRDVWFSFTTGDDILSFSIQVVGSVSGTNMAPIT
                     NPQIAVYRGDCALNGLAEISCISSPNGSNQTVLDILDLTPNTTYFLRINDYSATASPN
                     WGDFNVCITEYVPAFNIGDAPGSTACFGTVYDSGGPDAGYGNNENHTFTICPADFHQC
                     IEISVLDFNMENNLDRLVVFGGNSIAAPVLGTITGVNPVTQPFVIQASTNGCVTLQFI
                     SDGSVVGTGFELTWTCSPLECNGSSFDDPIVINNVPFSLTGATTCDDASNFNQSPCGN
                     DAFLNGPEDVYVFNSPGGICVGIQITGANAGTGVLVLNGPPGDPATVC"
     gene            complement(4949..>5424)
                     /locus_tag="PK198_15105"
     CDS             complement(4949..>5424)
                     /locus_tag="PK198_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HRF40117.1"
                     /translation="LDPSIGEFCLSHRDIQIPDRGNYYSVNQGYYLKFDLEMRRYIDH
                     CSDLNLQLRYIGSMVSDVHRILFQGGIFLYPNTRKYPQGKLRLVYECNPLSFIVEQAG
                     GKAITAQLERILELPVKHLHERVTIAIGSPGMVDEMKDFVERYSAPPMYKHAEAG"
     assembly_gap    5425..5434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5435..5982)
                     /locus_tag="PK198_15110"
     CDS             complement(<5435..5982)
                     /locus_tag="PK198_15110"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRF40118.1"
                     /translation="MNRIVTLDEFIIRRQMDFPFASGELTGLLRDIGVAAKIVNREVN
                     KAGLVNILGVAGSENASGETVQKLDLYANDKLIECLKNSGECCGVASEEFEDYIPIPG
                     VSSKTSKYVVVFDPLDGSSNIDVNVSVGTIFGIYRRVSDPEGPCKLADFLQPGTQLVA
                     AGYVLYGTSTLLVYTTGRGVNG"
     assembly_gap    6227..6322
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6323..>6597)
                     /locus_tag="PK198_15115"
     CDS             complement(<6323..>6597)
                     /locus_tag="PK198_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40119.1"
                     /translation="IGYFPGRIERDDWVGQAVKLMQNEPVATAEEPLDDKMYDLKVIE
                     GIGPKIEELLKSAGILHLPQLADTPVEVIEKILDQAGGHFRMHTPDT"
     assembly_gap    6598..6724
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6725..7336)
                     /locus_tag="PK198_15120"
     CDS             complement(<6725..7336)
                     /locus_tag="PK198_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40120.1"
                     /translation="MDNFFAHFDSQESMAILFFLILAFLLGLLTGYFLRSRRIAQMRK
                     ALEDSEKAHAEAQQQITALKAQLDLQNADLKKAMFEAEEQIARASRLEEDSTRQYNEM
                     LSLNAEIERLQGLQLSAGNSMEGLRAQIAGLQSQVGALDAEKTALMAALAQAEQTAAL
                     MAVAPVAELEPTLVFQKEKTVLNEKIDALSTAPAQRDNLDRIEG"
     gene            complement(7355..8233)
                     /locus_tag="PK198_15125"
     CDS             complement(7355..8233)
                     /locus_tag="PK198_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF40121.1"
                     /translation="MRALLIFLLFLAYALAVRWYYVCEMKGLCGAVVSDQVLEPQDVR
                     LKNLRLISGDTLLLNGYDQFAFNAGQVQPRLNDNNAAFLDSVAAIIKVAPGRKLTITG
                     RYAPQEDGKPYGFFENYGTARADALRRLLTQRGIDERLISLDHEPSRDSMLREPAVFE
                     IFYDAAAGVPEGYEKVQFSFTNMTFSDANFAFGSAEFKPGSAFIAYADSVKTYLQINP
                     ERRLSIVGHTDNVGSDKFNDGLGLRRAKSARTYFQELGVASEIQTQSEGKRKPVATND
                     TAEGRQKNRRVNFIIE"
BASE COUNT         1816 a         2070 c         2248 g         1801 t
ORIGIN      
        1 agattgatgt ctaagaaggc cgtatctaag cagccggccg ccgacttcac gatcagccga
       61 tagttaaccg aagtcactcc ggtagcaggt atgggggtat cggtaaatgc cgggccgccc
      121 atttgctctg cgccgccgtt cacgctccac agatacatcg cgcccacagg gtcgcccgga
      181 gtggtgttgg ccgtgatggt cactgccgag ccgatcacaa ccgaggagtc gccacgtgag
      241 gacaccaatg cgtcaatgcg cacgctttct tcaatcgtca ccaaaatggt atcgcgcacc
      301 gtagcgcata tcagattggc attcgggccg taagagtagt tgacgatgta catggccggg
      361 gagctcagcg cagcgctgct cgatacactc accgaagccg tgttgttgga accggtaatg
      421 cccgggccgc tccaggagta gctttgcacg ccgcccaccg cagttccggc agccgtcaga
      481 ttgaaaccta caggacggca caccgagaag tcctgaccgg cattcaccgt tgccggcgga
      541 gtgacggcta tgaccatttg atcttccgca gcggggcagg ggccatccgg gtcgttggtg
      601 cgcaaggtca gcgtcaccga agtcacgccg gcaggagcgg tataagtggc attcagcgtg
      661 ttggcattgg gattgaacac gccgccggga acggaggccg tccacacagc gctcgtggcg
      721 ccgccgccga tggttcccgc cagggtaacc ggattgccac tgcacactgc ctggtcgggg
      781 ccggcgttga cagtcgctgc cggattcact accaatgcca tctcatccgt tgcctgcggg
      841 caaacattgt tcgggtcggt ggctgtgaaa cgcagggtga tgataccggt tgttgtcggg
      901 gtgtaaaccg cgttggccgt catggcatta ggactgaaac tgccgccgga agccgttgtc
      961 cagatgccgc tcgttgccgc gccgccgaag gttccggcca acgtagccgt agaaccctga
     1021 cagatggtct ggtcggggcc ggcgtcggcc gtgggcggtt gtatcacggt gatggtgacg
     1081 gtataaaccg catcggggca accggcttta gaggcccgca gcgtataagt ggtggttacc
     1141 aaaggcgcag caatcaggag tgggtcattg gaagtaaaat tcggatcggt gctggatgtc
     1201 cacctgtaca acgattcatt gtcggcagcc agattgagtt gtaccggcgt gccgatacag
     1261 accgttcgat tgggcgacat gttcaccaca ggcggaatga cgaccctgat ggtggccgaa
     1321 gcgcctaccg gacagttggg cacgtccacc tggaaattat aggtaatggt tgcgccgggc
     1381 gttgccaccg gattggggca atcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nngcataggc ggctccacca cattgatcgt aatagagacg
     1561 gtatccacac agccgcgatg gatgatgacg cggcggtaag tggcggtgtc aatggccgaa
     1621 atgaccatat tccacaaggt atccggcgtt tcaaaacctg ggccgggcag ccattggaat
     1681 tcaatatccg ggaagtcgct cggctcgtag ttggtagagc gcaaaatgac gatatcgccg
     1741 gggcaatagg ggtcttttgc cgggtcggca atgatggcgc ggtcggcggg cagcgagtcc
     1801 acttcaatga caatgcggtc ggttttggag caacccggca cattcaccgt agcaaaatag
     1861 gtgatggtcg tctccggcgt agcctgtatg ctgttgcccg tgttggacga cagccatatc
     1921 gcaggcgtcc agcttgccac cgcgttagag ggggttatat ttaattgtaa atcaaccgtt
     1981 tcgcccagac atatccgcaa ggtatccggg ttgaggatgg caagatcaat gggggtaata
     2041 gtgaccgtaa aggtgtctga aacttcgcat acgccccgcg tggcgcgcag cacatagtcg
     2101 ccggagtttt gcggctgggc aacggtagtc gctgcatccg gattttcaat gtcatccggg
     2161 ccgctccatt ggtacgtcac atcctgctca gcagtggtat tggtcaacaa aatcggctgg
     2221 ccctgacaca cggcagtatc tgccgggatg gtgagggaag gtgtttgata aaccgtcaca
     2281 gtcacgctgt ccaacgaaga caaggggcac agcgagttgg tggccgagag gtagtaagtc
     2341 gttgtttgcg tgggcgatac ctgcgggttg gcctcgccgg aagcaaagcc gccgggcacc
     2401 gaactccagc tataggtgac atccggtgtg gcagccccgc caagattgac cgaagcgcca
     2461 acgcagattt ccacgttgtc gctgatggca aattgcggcg ccggttgtgg caatacattg
     2521 aatctgaggc tgtccgacaa aatacagttg ggtgtaatga gccgcacgcg cacgctatac
     2581 tcgccagaca cagagccttc caccattgga tcggggcaat cggtacaact gaaggtaaca
     2641 cccgcaggcg ccgtccattc gtaggtggca ttttcataat cagtaccgga tacgatttgg
     2701 atttgctctc cgcggcacac cgtgaggttc gggccggcgt ccacatcaaa cacggtcact
     2761 tttacatcaa tggtttcatc ataacaaacg gcgtcaatga gcgccgtgta agtggtggta
     2821 gccgagggcg tagctacagg attagggcag ttggtgcagg aaagtgttcc tgtaggatcg
     2881 gaccaactga tattcccgat ggcgctgatg acggagagtt gaacagatgc tccaatacaa
     2941 atcgttgtgt cgttgccggt cacaaccgga ggtggcggag ccaatacatc cggatcggaa
     3001 gtggaccaat ctacagagat accctcctct ccgatttggt tgccgaagtc gttggtcaga
     3061 atcacaaaca cctgcccttc caatgccgga atggtgcgta caaagcggtc gccgcctgcg
     3121 ccgggtgtag ccgggctgcc gcagtcgaag tcatccaata ctggcgcgcc ggtatcggga
     3181 ttgatgtcgg ccaaaccggt gcgggtactc cctcccgtcc acgaagagcg aatgggctgc
     3241 gtattggtta ctgcattgat gactacgccg ggcgtctcgc aaacttgctc ctgagtaaan
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggatgc tgcacctgca ccttccaaaa
     3361 tgaagccaaa cggtccgtcg gcctgcgcct gaatggtgaa ccagtaaaag tctgcttctg
     3421 cacctacgcc attggtaggg aagcaaccgg agtttacgtc ttcaatcagg tctatatcct
     3481 gaaatccatc ctgaaaatta aaaacagaag gcactccgcc ctcctcaata caaccattga
     3541 gcggattgca aagggcattc accaaaccgg cgggcagttc acactcggtg ggtgtgatgc
     3601 ttatattata tggtgtacaa ccgttgccgt ctgccactac gatgtagtag atacccggtt
     3661 cgcggaggtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacaaactg
     3721 tagccgggtc gccggggggg ccgttgagta ccaatacgcc ggtgcctgca ttggcgccgg
     3781 taatctggat acctacgcaa atcccgccgg gagaattgaa cacatacaca tcttccgggc
     3841 cgttgaggaa ggcatcgttg ccgcaaggcg actggttgaa attggaggca tcatcacagg
     3901 tggtggcgcc cgtgaggctg aaaggcacgt tgttgataac tataggatca tcgaaagagg
     3961 atccgttgca ttccaatgga gagcaagtcc atgtgagttc aaaacccgtg ccgactactg
     4021 agccgtcgga tatgaattgc aaagtaacgc aaccattagt agaagcctga ataacaaagg
     4081 gctgtgtgac gggatttacg cctgtaatgg tgccgagcac gggggcggcg atagagttgc
     4141 cgccgaagac gactaagcgg tccaagttat tttccatgtt aaaatccaac acagaaattt
     4201 caatacactg atgaaaatcc gccgggcaga tggtgaacgt atggttttcg ttattgccat
     4261 agccggcatc cgggccgccg gaatcatata cggtgccgaa acaagcagtg ctgccgggcg
     4321 catcgccgat gttgaaagcg ggcacatact cggtgatgca cacgttgaaa tcgccccaat
     4381 tgggggatgc cgtcgccgaa taatcgttga tgcgcaaaaa ataagtggtg ttgggcgtga
     4441 gatcaaggat gtccagcacc gtctgattgc tgccgttggg cgaggaaata caggagattt
     4501 ccgccagccc gttgagcgcg caatccccgc gataaacagc gatctgcggg ttggtaatgg
     4561 gggccatatt agtgcctgag acacttccga cgacctgaat ggaaaaactg agaatgtcgt
     4621 cgccggttgt gaaagagaac cacacgtctc gctgggcggt gccgccattg aagcaagagg
     4681 ggttattccc gaagccgatg ttggtagccg tagcgtttac atttgtgtaa accgtttgcg
     4741 ggcagatggg cgctaccccc agatcaatca atccgttaca attgtcgttg atcggggcct
     4801 gagccgtcac gaagcctgaa aaggcaataa atacaaggaa agcgtagaaa aacttcattg
     4861 gttgtacgat ttggaaagac caaagttaaa cataatttga aatttccaac tttccgatga
     4921 aattagtgtc gccggccttg cattcgcatc accctgcttc tgcgtgttta tacatgggcg
     4981 gcgcgctgta tctttctaca aaatctttca tttcatccac catgccggga gagccgatgg
     5041 caatggtgac gcgctcgtgc aggtgcttga cgggcagttc caaaatgcgc tccaactgcg
     5101 cggtaatggc tttgccaccg gcctgttcta caataaaaga cagcgggttg cactcataca
     5161 ccaagcggag tttgccctgc gggtacttgc gggtgttggg atacaaaaag atgccgccct
     5221 gaaacaagat gcggtgtaca tcgctcacca tcgagccgat gtaccgcaat tgcagattga
     5281 ggtcggaaca gtggtcaata tacctccgca tttcgaggtc gaatttaagg taatacccct
     5341 gattgacaga gtaatagttg cccctgtctg gaatttgtat gtcgcggtgc gagaggcaaa
     5401 attccccgat ggaggggtcc aatgnnnnnn nnnnaagccg ttcacgccac gtccggtggt
     5461 ataaaccagc agcgtggagg tgccgtaaag cacatacccg gctgcgacca attgtgtgcc
     5521 cggttgcaaa aaatcggcta gtttgcaggg cccttccggg tcgctcactc ttctataaat
     5581 accgaaaatg gtgcccacgg acacattgac atctatgttg gaggagccgt ccaaggggtc
     5641 aaaaacgact acatatttgg atgttttgga actgacgccg ggaatgggga tgtaatcctc
     5701 aaactcctcg gaggccacgc cgcaacattc gccgctgttt ttcagacatt cgatgagttt
     5761 gtcgttggcg tacaggtcta atttttgcac ggtttccccg gaggcgtttt cactgcccgc
     5821 cacgccgagt atattcacaa ggcccgcttt gttaacttcg cggttgacga ttttggcggc
     5881 cacgcctatg tcgcggagca ggcccgtcag ctcgccggag gcaaagggaa agtccatctg
     5941 tctgcgtatg atgaactcgt ctaatgttac aattcggttc atgtttggtt ggtcttatgg
     6001 ttttggcaaa aaaagtgaat attgcgcggg ttctacccgc ctgcaaaatg cctcattgca
     6061 ctcgttgcac cgacttcgcc ttcgggccta caaacacaat cgcataagct gccagattcg
     6121 ggatgcggcg caggtcatcg gtttccagat aggcttccaa ttgcatgcgg gcttcttcgg
     6181 gcttcgcagc gagttgttta gactctgcta aaaaaacacg cacctcnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnaggtatcg ggagtgtgca tgcgaaaatg acctcctgcc
     6361 tgatccaaga ttttttctat cacttccacc ggcgtatcgg ccaattgcgg caagtgcaaa
     6421 ataccggcgc ttttcagcaa ttcctcaatc ttcggcccga tgccttcaat tactttcaga
     6481 tcatacattt tatcatccaa aggctcctcg gcggtggcga caggctcatt ttgcatcagt
     6541 ttcacggcct gccctaccca gtcatcgcgc tcaatgcggc ccggaaaata gccgatcnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnncccttc gattcggtcc aggttgtcgc gctgggcagg agccgttgaa agtgcgtcaa
     6781 ttttttcgtt gagcactgtt ttttcttttt gaaaaaccaa agtcggctcc aactcggcca
     6841 caggagcaac tgccatcagc gctgcggttt gttctgcctg cgccagggca gccatcaagg
     6901 ccgttttttc agcatctaag gcgccaactt gagattgcag acccgctatt tgtgcacgca
     6961 gcccttccat cgagttgcct gcactcagtt gcaacccttg caggcgctca atttccgcat
     7021 tcagcgaaag catctcgttg tattggcggg tactgtcctc ttccaatcgg gaggcgcgcg
     7081 caatttgctc ctctgcctca aacatggcct ttttcagatc ggcgttttgc aggtcgagtt
     7141 gcgctttcag ggccgtgatc tgttgttgtg cttcggcatg ggctttttca ctgtcttcca
     7201 aggctttgcg catctgagct atgcgtcggc tgcgcaggaa gtatccggtg agcaggccca
     7261 ataaaaaagc caagatgaga aaaaacagga tcgccatgct ttcctgagaa tcgaaatggg
     7321 cgaagaagtt gtccatattg agttgatctt tagattattc gatgataaaa ttgacacgac
     7381 ggtttttctg gcggccttcg gcggtgtcat tggtagccac aggtttgcgc ttgccttccg
     7441 actgggtctg aatttcggag gcgacgccga gttcctgaaa ataggtccgg gcgctttttg
     7501 cacgtcgcag ccccagcccg tcattgaact tgtcggaacc cacattatct gtatggccta
     7561 cgatgctgag gcggcgctcc gggttaatct ggaggtaggt tttcaccgaa tcggcatacg
     7621 ctataaaggc cgaacccggc ttgaactccg ccgaaccaaa agcgaaattg gcgtcggaaa
     7681 aagtcatatt ggtaaacgaa aactgcactt tttcataccc ttcgggcaca ccggcagccg
     7741 catcgtagaa aatttcaaaa acagcgggtt cgcgcagcat ggaatcgcgg cttggctcat
     7801 ggtctaagct gatcagtcgc tcgtctatgc cgcgctgtgt caacaaacgc cgcaaggcat
     7861 cggcacgggc agtgccgtag ttttcaaaaa atccgtacgg tttcccgtct tcctgcggcg
     7921 cataacgccc ggtgatggtg agctttcggc ctggtgccac tttgatgatg gccgctaccg
     7981 aatcgaggaa tgccgcgttg ttgtcgttca ggcggggctg cacctgacct gcattgaagg
     8041 cgaactgatc gtagccgttc agcagcagcg tatcgccgga aatgaggcgc agatttttca
     8101 gtcgaacatc ttgtggttca agtacttgat ccgacacaac agcgccgcat aagcctttca
     8161 tttcacacac ataataccac ctcacggcca gtgcataagc caaaaataaa agaaaaatga
     8221 gtaaagctct catcccgtca ttgctttgtt ggcaataaaa gtacatatct tggcggtact
     8281 ggcaaatttt tttgtaaaaa tgcgccggtc ttgatgcgct gctgccaaca aaaacagccg
     8341 gaacgatctc gtcccggctg
//
LOCUS       DAORLF010000197         8356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4612_length_8356_cov_1.819781, whole genome shotgun sequence.
ACCESSION   DAORLF010000197 DAORLF010000000
VERSION     DAORLF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4612_length_8356_cov_1.819781"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>248
                     /locus_tag="PK198_15130"
     CDS             <1..>248
                     /locus_tag="PK198_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF40122.1"
                     /translation="YIFEAVEVEKAGLKVQKSTAPELPKELLRKFGEMPAFREAFEAL
                     TPGRQRGYLLHFSQPKQSATRTTRIEKCLPKIFAGKGM"
     assembly_gap    249..376
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(416..>628)
                     /locus_tag="PK198_15135"
     CDS             complement(416..>628)
                     /locus_tag="PK198_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40123.1"
                     /translation="LLVSAIPSEDGRSAIFQVREIGGKTAVLTLQNHLSGAPLDVQRV
                     DATGWPVPKGGAQLEAWESGFFRVRW"
     assembly_gap    629..646
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<647..>982)
                     /locus_tag="PK198_15140"
     CDS             complement(<647..>982)
                     /locus_tag="PK198_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40124.1"
                     /translation="ARLWNDAAQIVLSSAEIPLMQFGGINTGRYQAGAKPAGTHLFGW
                     PMNNYWTTNFNADQHGGHTWLYSLTSAAGHALQDAARFGWGHRTPFLGRMMPGGGRGD
                     AQWQGSYLR"
     assembly_gap    983..1020
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1021..>1868)
                     /locus_tag="PK198_15145"
     CDS             complement(<1021..>1868)
                     /locus_tag="PK198_15145"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRF40125.1"
                     /translation="KEAYAWEAARRAKMLGAETMGLLQSYVAREQEPALIVYNTLNWA
                     RSGLVKVYIDHQILPRFTPFTLTDQSGKTIQAQPVEHHSDGTYWAVWVDDVPAFGYKK
                     WVLRLRKNEKTPPPIRLAQSPQVLENEWYKIEIDTAKGAVAGILDKETGLQLVDKKAP
                     WKLGEFIYERLDNRAQMEAFKLDNFTREGLEKVWFDASEPGDVWDALRFRGDTRAAHS
                     QGGFELEIRLFHTAKRIDLAFSIEKKLVTDPEGIYIAFPLALDDGQLAFDVQGGEVRA
                     GIDQIPG"
     assembly_gap    1869..1882
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1883..>3077)
                     /locus_tag="PK198_15150"
     CDS             complement(<1883..>3077)
                     /locus_tag="PK198_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40126.1"
                     /translation="RLPVVQNPTTVTYRLDVAGRSWKGKLELRPVRHWRVNFVQHSHT
                     DIGYTRSQTEILAEHLRYIDYALDYCDLTDNYPDAAKFRWTCEASWAVDEYLRSRPRQ
                     QIERLKKRVAEGRIELTGMYFNFNELPDEATLAASLQPLRRIREQGMPLKTAMQNDVN
                     GIGWSLCDYFSDLGVRYLNMGTHGHRALICFDKPTLFWWESPSGKRMLAFRAEHYMTG
                     NTVFKIHAGDFNVFESELLAYLAALEAKGYQYDLISIQHSGYLVDNAPPSTMASDMIR
                     QWNEKYTWPKLKTASATEFFEEMEQKHSADFQTIRGAWPDWWTDGFGASAREVAATRM
                     AQSDLIANTAALTMAALNGAEMPSKIADRIGETNSALLFYTEHTVGYHASVREPFHRY
                     TMEQRA"
     assembly_gap    3078..3117
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3118..3862)
                     /locus_tag="PK198_15155"
     CDS             complement(<3118..3862)
                     /locus_tag="PK198_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40127.1"
                     /translation="MHAFHLKIKRNAMQAARFLLPILCIFGLSATLPAPAQAQDKALI
                     VNGFERKISGDDFWYHSSIPVAKSSLLIRATDGQSSMEWATAPALMLTKEERVNFVWL
                     AGIGSSPGKASFDLAVNGVQQFTFWADGADEWKLDAPDGSTLSFRKDMIDQHGDRFGF
                     MTLSVPAASLRTGQPLSIRVIGGRFNKTSWYMTFQFPIESGLNISALPAVFQQAGQSH
                     QLGVAGILHFGAPTTAKVWLDGRLIEQTPL"
     gene            complement(3958..>4414)
                     /locus_tag="PK198_15160"
     CDS             complement(3958..>4414)
                     /locus_tag="PK198_15160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF40128.1"
                     /translation="AAPAEKVSWLGIRSSESWRKVGTSFAIIITLATGAFMYFGVYQN
                     TPIQWMPALPWVLLFSISNSFSEEMLTRFSLAAGLDGLLSGRTISLWSAAIFGGVHYF
                     GTPGGVPGVLMAGYLWVLAKSVLETKGFGWAWFIHFLQDVVIIFALVGR"
     assembly_gap    4415..4432
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4433..4634)
                     /locus_tag="PK198_15165"
     CDS             complement(<4433..4634)
                     /locus_tag="PK198_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40129.1"
                     /translation="MKKAYLIIPVVALATLALLPLNRVVAPLLPLSVSENPAVNRMTA
                     AQLLTLTLTGLVLLFIGAVNRQG"
     gene            complement(4779..>5244)
                     /locus_tag="PK198_15170"
     CDS             complement(4779..>5244)
                     /locus_tag="PK198_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF40130.1"
                     /translation="VKESGDTERYLSFVVAGVGGVFAWRDEREVCLDFSFENQFFGDY
                     ASFLRQGPSPVFTQAVEPLEMYSIAHHDLEQLYAASPEGIRLARAAAESLFIESQQRQ
                     LDLLTKTAEERYRELLAHYPDALLRLPQKHIASWLGITPESLSRIRKAVASV"
     assembly_gap    5245..5269
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5532..>5810
                     /locus_tag="PK198_15175"
     CDS             5532..>5810
                     /locus_tag="PK198_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40131.1"
                     /translation="MEKGTKENPWQLKTAPGTAEYQMWRDDEKSIIVCKVGSTILHYD
                     QRCIEDTVAMLQAHGDWMELGAADEQKEAKPGTVEAWGRSPENPLGGWY"
     assembly_gap    5811..6038
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6263..6408
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6411..7346)
                     /locus_tag="PK198_15180"
     CDS             complement(6411..7346)
                     /locus_tag="PK198_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRF40132.1"
                     /translation="MDNNLIINWLCEGDVAIQYQTYRDLMGVPRPDLQQRISSEGWGR
                     AFLEKQNPDGHWGQKYYQPKWVSTHYTLLDLKNLQIKPDLPQIRQIIDRVLKTEKGPD
                     GGVLPIGTSKRSDVCVNGMLLNVAAYFRAEEADLQSIVDFLLSERVADGGFNCRSNGQ
                     GCVHSSLHSTLSVAEGILEYQQNGYAYRLPELLAAAATSQEFMLMHRLFKSDKTGAVI
                     DVRMTKLPYPSRWKYDILRALDYFQKAALPYDARMEDALQLLLQKRLPEGRWKLQSGY
                     AGQVHFNMEKAGQPSRWNTLRALRVLRRYGETMFS"
     gene            complement(7336..8112)
                     /locus_tag="PK198_15185"
     CDS             complement(7336..8112)
                     /locus_tag="PK198_15185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF40133.1"
                     /translation="MHILSITADNINREHICCAIGNDKENTRRAQGKKDWLSNRFSEG
                     LIFKRFDARGKIFVEYVPIETVWKPLVGKNYMVINCLWVSGQFKGQGLSKQLLDECLH
                     DARSAGMDGIAVVTSTKVKPFLTDKKFYLRHGFETVDTAPPYFELLALKFRPDALNPH
                     FADHVKNGSTHQARPGFTFVYSNQCPFMEDYGGRVYVEMARQHGFEAQAIQLKNFEEA
                     QQLGSPFGTFGAWFDGAFQFHELMTEAKFEQYLNSLKNGQ"
BASE COUNT         1729 a         2041 c         2210 g         1721 t
ORIGIN      
        1 atacattttt gaggccgttg aagtggaaaa ggcgggactg aaagtgcaaa aaagcactgc
       61 gccggaacta ccgaaagaac tgttgcgcaa gttcggagag atgcccgcat ttcgggaggc
      121 cttcgaggcg ctcacgccgg ggcggcagcg aggctatttg ttgcactttt cacaacccaa
      181 gcagtcggcc acccgcacaa cgcgcatcga aaaatgcctg ccgaaaatct ttgcagggaa
      241 gggaatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnntgga caaatatcca agcggatgct ttttcgggca gccccttacc
      421 accgcaccct gaaaaaacca ctctcccagg cctccaactg cgcgccgccc ttcggtaccg
      481 gccaaccggt ggcatccacc ctctgcacat ctaagggagc accgctgaga tggttttgca
      541 aggtcagcac agccgttttt ccacctattt cccgcacttg aaaaatagcc gagcgaccat
      601 cttcggaagg tattgcactc actaagagnn nnnnnnnnnn nnnnnncgcg gaggtaggaa
      661 ccctgccact gcgcatcgcc gcgaccgccg ccgggcatca tccgccccaa aaacggcgtg
      721 cggtgtcccc aaccaaagcg cgccgcgtct tgcaaggcat ggccggcagc gcttgtcagc
      781 gagtagagcc aggtatgccc gccgtgctgg tcggcattga aattggtggt ccagtagttg
      841 ttcatcggcc agccgaagag gtgagtgcct gcaggcttgg cgccggcctg gtagcgcccc
      901 gtgttgatgc cgccgaactg catgagcgga atctcggcgg aactgagcac aatctgcgcc
      961 gcgtcgttcc agaggcgggc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 ccgggaatct ggtctatgcc ggcgcgtact tcgccgccct gcacatcaaa agccaattgg
     1081 ccgtcgtcga gggccagcgg aaaggcgatg taaatgccct ccggatcggt gactaatttt
     1141 ttctcgatgg agaaggcgag gtcaatgcgc ttggccgtgt ggaacagcct gatttccagt
     1201 tcaaaaccgc cctgactgtg cgcagcgcgg gtgtcgcccc ggaagcgcag ggcatcccac
     1261 acatcgccgg gttcagaggc atcgaaccag actttttcca agccttcgcg ggtgaaatta
     1321 tcgagtttga aagcctccat ctgagcgcgg ttgtctaagc gttcgtaaat aaattcgccg
     1381 agtttccagg gcgctttttt gtccacgagt tgcaggccgg tttccttgtc gagaatgccg
     1441 gcaacagcgc ctttggccgt gtctatttca attttatacc attcgttttc aagtacttgc
     1501 ggcgattggg ccaacctgat gggcggcggc gtcttttcgt tcttgcgcag ccggagcacc
     1561 cattttttgt agccgaaagc gggcacatca tccacccaga cggcccagta agtgccgtcg
     1621 gaatgatgct ctacgggctg tgcttgtatg gtttttccgc tttgatctgt gagggtgaac
     1681 ggcgtgaagc gcggcaaaat ctggtggtca atatacactt tgaccaagcc actgcgcgcc
     1741 cagttgaggg tattgtacac gatcaaagcc ggctcctgct ctcgcgcgac gtagctttgc
     1801 aacagcccca tcgtttcggc gcccagcatt ttggcacggc gggcggcttc ccaggcgtag
     1861 gcctccttnn nnnnnnnnnn nnggcccgtt gctccatggt gtagcggtga aaaggctcgc
     1921 gcacactggc gtggtagccc acggtgtgtt cggtgtaaaa caacagggcc gagttggttt
     1981 ccccgatgcg gtcggcaatt ttggagggca tttcggcgcc gttgagggcg gccatcgtca
     2041 gtgcggcggt attggctatg aggtcgcttt gagccatgcg ggtagcggcc acttcgcggg
     2101 cggaagcgcc gaatccgtca gtccaccagt cgggccaggc gccgcgaatg gtctgaaaat
     2161 cagcgctgtg tttttgttcc atttcttcaa aaaactcggt agcagaggca gttttcagtt
     2221 tcggccaggt gtatttttcg ttccactggc ggatcatatc ggaggccatg gtggagggcg
     2281 gcgcgttgtc caccagatag cccgaatgtt ggatggaaat gagatcgtac tggtagcctt
     2341 tggcctctaa ggcggcgagg taggccagca gttccgactc aaagacgttg aaatcgccgg
     2401 cgtgaatttt gaaaaccgtg ttgccggtca tgtagtgttc ggcgcggaaa gccagcatgc
     2461 gtttgccgga aggcgattcc caccaaaaga gcgtaggctt gtcgaaacag atgagcgcgc
     2521 ggtggccgtg ggtgcccatg ttgaggtacc ggacgcccag atcgctgaaa taatcgcaaa
     2581 ggctccagcc gatgccgttg acgtcgtttt gcatggcggt tttcaggggc atgccttgtt
     2641 cccggatgcg gcgcagtggt tggagtgagg ccgcgagggt ggcttcgtcg ggcagttcgt
     2701 tgaaattgaa atacatgccg gtgagttcga tgcggccttc ggccacgcgc tttttcagcc
     2761 gttcgatttg ttgccggggc ctgctgcgga ggtactcgtc cacggcccag gacgcctcgc
     2821 aggtccacct gaatttggcc gcatcggggt agttgtcggt gaggtcgcag tagtcgaggg
     2881 cgtagtcaat gtagcggagg tgttcggcta agatttccgt ttgcgagcgg gtatagccga
     2941 tgtcggtgtg ggaatgttgc acaaaattga cgcgccagtg ccgcaccgga cgaagttcca
     3001 gtttgccttt ccaggaacgg ccggccacat cgaggcgata ggtaactgtg gtcggatttt
     3061 gtacgaccgg caggcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
     3121 ggggcgtttg ctctatgagt cggccgtcga gccagacctt ggccgttgtg ggcgctccga
     3181 aatgcaggat gccggccacg ccgagttggt gcgattggcc ggcctgctga aagaccgccg
     3241 gcagcgccga gatgttcagt ccgctctcaa tgggaaactg gaaagtcatg taccaggagg
     3301 tcttgttgaa acggccgccg atgacgcgaa tggacagcgg ctgcccggtg cgaagggagg
     3361 cggcaggcac tgagagggtc ataaaaccga agcggtcgcc gtgttggtca atcatgtctt
     3421 tgcgaaaaga gagcgtagag ccgtcgggcg cgtcgagttt ccattcatcg gcgccatcgg
     3481 cccagaaggt aaattgttgt actccgttga ccgccagatc gaaactcgct ttgcccggcg
     3541 aagaaccgat gcctgccagc catacaaagt tcacgcgctc ttccttggtg agcatcaggg
     3601 cgggcgcagt ggcccattcc atagaggatt ggccgtcggt ggcgcggatg agcaggctgg
     3661 atttggctac aggaatggag gagtgatacc agaagtcgtc accggaaatt tttcgttcaa
     3721 atccattgac gatgagtgct ttatcttgcg cctgggccgg cgcgggcagg gttgccgaaa
     3781 ggccgaaaat gcaaaggata ggcaggagga aacgggcagc ttgcatggcg ttgcgtttta
     3841 ttttaaggtg aaaggcatgc aaggatagtg aattttgggg agatgaaata tctgagtgga
     3901 gataacagat tgtacaaaat cagacgcttt gatggacagg agaactggat ttgcagccta
     3961 cctccccacc agcgcaaaaa tgattaccac atcctgcaaa aaatggatga accaggccca
     4021 accgaatcct ttggtttcca acacagattt ggccagcacc cacaggtacc ccgccatcag
     4081 cacgcccggc acgccgcccg gagtgccgaa gtaatgcacg cccccgaaaa tagcggcgct
     4141 ccacaggctg atggtccgcc ccgacaggag gccgtccaaa ccggccgcca gactgaatcg
     4201 ggtgagcatt tcttctgaaa atgaattgct tatactgaac agcagcaccc agggcagcgc
     4261 cggcatccat tgaatgggcg tattttgata aaccccgaaa tacatgaaag cgcctgttgc
     4321 caatgtaata atgatggcaa acgaagtccc cactttgcgc caggattcgc tcgaccggat
     4381 gcccaaccag ctcacttttt cagccggagc tgctnnnnnn nnnnnnnnnn nnacccctgc
     4441 cggttgacag ctccaatgaa aagcaatacg aggccggtca gcgtcaacgt gagcagttgg
     4501 gctgccgtca tgcggttgac ggcaggattt tcggaaacag acagcggcag cagcggagct
     4561 actacgcggt tcagcggcag cagcgccagg gtggccaatg cgacgacagg tataatcaga
     4621 tatgcttttt tcatggcagg agtatgattg ttcatttgat cctgcaaaga tggcggggtg
     4681 ggtagcgata aaaaattgat gtatggtaag aaaaatggaa tgtcgtccct attgaaggag
     4741 tgactgccag tcactccttc aatagccaaa ttttacactc acacagaagc caccgccttc
     4801 cgaatgcggc tcagactttc gggggtaatg cccagccagg aagcgatgtg tttttggggc
     4861 agtcggagca gggcgtcggg gtagtgcgcc aaaagctccc ggtagcgctc ttcggcagtt
     4921 ttcgtaagga ggtcaagttg tcgttgctgg ctttcgatga acagcgactc ggcagcagcc
     4981 ctcgccagac gaatgccctc cggcgaggcc gcatacaact gctccaaatc gtgatgagcg
     5041 atgctgtaca tttcgagcgg ctccacggct tgggtaaaaa cagggcttgg accttgccgc
     5101 agaaacgaag cataatcgcc aaagaactga ttttcaaaag aaaaatcgag gcaaacctcc
     5161 cgttcatccc gccaggcaaa cacgccgccc acaccggcaa caacaaaaga aaggtagcgc
     5221 tctgtgtcgc cggactcctt tacgnnnnnn nnnnnnnnnn nnnnnnnnnc atttttttca
     5281 aatcgccgga agtgcatacg ctctgcaaaa ccctcccaca gcgccgccgg agccggttgg
     5341 aatgggttga aaaattggcc gatgcggtcg gaaagagaag ccatgacgag catattgaag
     5401 gtgcaaaatt ggcctttttt gttaaaaatg ctgcacatgc ggccatcatt tgcctgtgcc
     5461 gtaaaaaaac cgtaccttgc tctcattaat gaccattgcc ggcattcacc tcactctcaa
     5521 atatttatac catggaaaaa ggaaccaaag aaaatccctg gcagctcaaa accgctcccg
     5581 gtaccgccga ataccagatg tggcgagacg atgaaaaaag catcatcgtg tgcaaagtag
     5641 gctccactat attgcactac gaccagcgct gcatcgaaga caccgtggcc atgctgcagg
     5701 cgcacggcga ttggatggaa ttgggcgctg ccgacgaaca aaaggaagcc aaaccaggca
     5761 cggtggaggc ctggggccgc tcgcctgaaa atccgctggg cggctggtac nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaagcgccgg cgggcagttg
     6061 gtaggtttgg ccggtggcct gtggcaggct gccgtcggta tttttataaa tgccgttcac
     6121 cacaaactcg caggcgatgc ggtggccgct ctcatccgac atcaacacca tatcactgag
     6181 ggtttcctcg tagcaatgat ccatgtgagc caaaaaatcc cggtaccggt ctttgaccgt
     6241 gcgcgttcga ccctgactga ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ttaggaaaac
     6421 atcgtttctc cgtacctcct caatacccgc agcgcccgca aggtattcca gcgactgggc
     6481 tgcccggctt tttccatatt gaaatgcact tgcccggcgt atcccgactg taatttccag
     6541 cggccttcgg gcaggcgttt ttggagcaac aattgcaagg catcctccat gcgggcgtcg
     6601 tagggcaagg cagccttttg gaaataatcc aacgcgcgca aaatgtcgta tttccaacgg
     6661 ctcggatatg ggagtttggt cattctgaca tctatgacgg cgccggtttt gtctgattta
     6721 aataggcggt gcataagcat gaactcttgc gaggtggcag cagcggccaa caattccggc
     6781 aatcggtaag cgtatccgtt ttgctgatat tccagaatgc cctccgccac cgagagggtc
     6841 gaatgcagcg agctgtgtac gcagccttgt ccattcgatc tgcaattgaa tcctccgtcg
     6901 gccacccgtt ccgacaataa aaagtctaca atagattgca aatcagcctc ctcggcccga
     6961 aaataggcag ctacattcag caacatgccg ttgacgcaca cgtcgcttct cttgcttgtt
     7021 ccgatgggga gcacaccgcc gtcggggcct ttttcggttt tcaaaacgcg gtctatgatc
     7081 tggcggattt ggggcagatc gggcttaatt tgcaggtttt tcagatcgag cagggtgtag
     7141 tgcgtcgaaa cccatttggg ttgataatac ttttgtcccc aatgcccgtc ggggttttgt
     7201 ttttccaaaa aggcccggcc ccagccctcg ctgctgatgc gctgctgcaa atcgggcctc
     7261 ggcacaccca tcaagtctcg gtaagtttga tattgaatgg ctacatcgcc ttcgcacaac
     7321 cagtttataa tgagattatt gtccattttt caagctattg agatattgtt caaacttcgc
     7381 ctccgtcatc agttcgtgaa actgaaaagc tccgtcgaac caggcgccga aagtgccgaa
     7441 agggctgccg agttgctgcg cttcttcaaa atttttcagt tggatggcct gcgcctcaaa
     7501 gccatgttgc cgggccattt ctacataaac cctgccgccg taatcttcca taaacgggca
     7561 ttggttggaa taaacgaacg taaaaccagg cctcgcctga tgggtgctgc cgttttttac
     7621 atgatcggca aaatggggat tgagcgcatc gggtcggaac ttcagggcca gcaactcgaa
     7681 ataaggcggc gctgtatcca ctgtttcaaa accatgccgg agatagaatt ttttatcggt
     7741 cagaaagggc tttaccttgg tactcgtcac tacggctata ccatccatcc cggcagaacg
     7801 ggcatcgtgc aggcattcgt cgagcagttg tttggacaaa ccctgacctt tgaactgacc
     7861 ggaaacccac aggcagttga tgaccatata atttttaccc actaatggtt tccacacggt
     7921 ttcgatgggc acgtattcga cgaaaatctt gcctcgcgca tcgaaacgtt tgaaaatcaa
     7981 gccttccgaa aagcgattgc tgagccagtc ttttttgccc tgggcgcggc gcgtattttc
     8041 tttgtcgttg ccgatggcgc agcaaatgtg ctcgcggttg atgttgtcgg cggtgatgga
     8101 gaggatgtgc atggcggcat ttttggaagg aatcgaagtg ccgggctatt cgggatttgc
     8161 gactgaattt gccggcaaga acggctcccg caggatggtt ttcagttttt cgcctgatga
     8221 ccaaactgag gaatctaggt tcaacattct gtcatcggtc atgacttgag cggagcgcgt
     8281 ccttttcacg atgaaccact gaaagtctta ttgctcactc actttcgttg ggcaataaca
     8341 cctcaagaca cgcgtc
//
LOCUS       DAORLF010000198         8308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4645_length_8308_cov_2.033200, whole genome shotgun sequence.
ACCESSION   DAORLF010000198 DAORLF010000000
VERSION     DAORLF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_8308_cov_2.033200"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>233)
                     /locus_tag="PK198_15190"
     CDS             complement(<1..>233)
                     /locus_tag="PK198_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40134.1"
                     /translation="NPVIPNIDPARAGSYMVTISGISGCTVSGTVEVAIENLPLIPLL
                     VTDTEICTNENIVLSSGIVVPGQGAVYNWYEGL"
     assembly_gap    234..255
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<256..>1490)
                     /locus_tag="PK198_15195"
     CDS             complement(<256..>1490)
                     /locus_tag="PK198_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF40135.1"
                     /translation="IFDPGAYLITYYATDCAGNQDSCSHFIVVEDLEAPVLTCPADIT
                     VSAGLDSCSAVVTLPLPVSVSDNCSVGAPFSQIQPTINTNALLTFEYDPNLNDRVAQP
                     KIYTFTGVAANATEDAVLSIIAQGDFNTNGAFVRVFGEDNTLLGTTTQGFANCDTAGQ
                     VAFTIPQALFNNWASDGQLHIRIVPNPILVPPGLPGDGINPCNPSEVTFDGDTDGASY
                     VFAQLEYAALEVAYFSTGATILPPTPLSASAPVTHEFVVGTTEIFYTTTDRAGNSDTC
                     SYRIIVQDTQRPVAACRPSIVYVNPSGVSNQSIDPALIDNGSTDNCGIASLSLSPNIF
                     SCNQAGMSFPITLTATDMAGNTDTCSTLIRIEMEAPQPTANSGICGGDTLFLFANPPA
                     AQGGIIYTFQWSGPQGFVS"
     assembly_gap    1491..1529
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1530..3808)
                     /locus_tag="PK198_15200"
     CDS             complement(<1530..3808)
                     /locus_tag="PK198_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40136.1"
                     /translation="MKIKSTAVILAALAALFLTSSLYGAPAGSRPLSDPPVFTNAANV
                     PDTLTFACLKDVPNGEKLEATDDNDPAFPQLIAPVDNPLPSSINGCTGGSFERVWTAT
                     DLDGNVTTLTQVIIVLPDTAPPVTTAVPVNDILPCEAINYSAWLAGVQLAFLSNTTDN
                     CSAIGMGLNFSNNAPPTRPPGCGTLTIQFTATDACNNTLNWPVTLTATDTIKPVLVGI
                     PLTDTIRCDQPLPPLPVVTATDNCSGDVDIVFTEVNNRAVNPADCGHYQYQVVRSWVA
                     TDACGNRSSASQIIRVLDDAAPDFTRPANITILCTDDSNDLGLTGNLSNITDNCSAAF
                     ATSSYQNQISPGNCPGNYTLQRLWRVQDVCNNSTVKIQVITVKDTVPPSFTVPADISI
                     RCDQNDNPTVTGFPTGITDNCQTGGTILPPSDVLLPGACINSYTIRRTWTVRDSCGNE
                     ASQIQLIFVSDSIAPVVQDEAQNLEVFCASNSDYQAAFTQWIQNRGGATASDNCTFSG
                     DLVWSAFNAGTNDPAALPAVSCPAADSVVLSQTVIFVVTDECGNRDSTTAVFRVIDNL
                     PPFFVQCPSDMLFPTDPGQCSALVELIPPVIGEDCGFGILSETASDQQPITSNAAPDQ
                     LGSTPVNAIDLNIPIGLPQPLNTAGPATLSVSLFSADAEGATEFFFIYDENGNLLDST
                     AYTPAQCGDSNVDILITPQQLDAWAADGVVRIRLEPNIPSGMSGAFAVNAVCINPGSA
                     RATLSYSARDLNGLRYEYS"
     gene            complement(4061..4264)
                     /locus_tag="PK198_15205"
     CDS             complement(4061..4264)
                     /locus_tag="PK198_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40137.1"
                     /translation="MAKKNQQQQDESAQNLQGFSIRINEFGQVETTMAIDRVNAYLNE
                     NVDDKKFRDKKEKDEAAVEDAAE"
     gene            complement(4496..7897)
                     /gene="galA"
                     /locus_tag="PK198_15210"
     CDS             complement(4496..7897)
                     /gene="galA"
                     /locus_tag="PK198_15210"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HRF40138.1"
                     /translation="MKSVLPMLLVLFFFCSQAHAQELSPRRHLNFDTDWKFAFGHAEN
                     PEKDFRFSIEPLFKKSGEALNTAVDPRFNDSLWRSLDLPHDWAVELPFVNSPDFNVMA
                     HGYKPVGGKFPETSIGWYRKRFTVNAADEGRRFELQFDGVFRDAHFWLNGMYLGNNPS
                     GYMGVAWDITDFIRYGEENVLVVRVDATQYEGWFYEGAGIYRHVWLNVFDQTHIVNDG
                     IFAYSEIKDGKAVISVETTLENKGNRKSDISIETYLTDNQGKIVAKTETKNLSVNILE
                     NQSILQKLTVNNPVLWDLDNPYLYRIHVNIKSNGQVTDRQILRFGVRKIEVKTNGVFL
                     NGKHVKLYGVNCHQDHAGVGSALPDYLQYYRIRLLKNMGVNAYRTSHNPPTPELLDAC
                     DSLGMLVMDETRLLNSSPEYLGQFERLLRRDRSRTCVFMWSIGNEEGWIHTSPTGKRI
                     AETLLKKQQQLDPTRTSTYAADLPNVFEGINEIIPVRSFNYRHFAVADYHRDHPHQPI
                     IGTEMGSTVTTRGQYERDSIRGYVPDQDISAPWWASTAEDWWTLAADNDFWLGGFIWT
                     GLDYRGEPTPFAWPNINSHFGIMDMCGFPKNIYYYYQSWWTDNDVLHISPHWNWMDRR
                     SDSLDVWVNTNADDVELFLNGKSLGKKDMPRNSHLKWKVKYEPGKLEAVAFKKGKKLT
                     AKVETTGTPFDVVLTPYKTTMVADGQDATVINVTVFDREGREVPNADNLIQFHIEGDG
                     KIIGVGNGDPSCHEPDKCADGRWQRSLFNGKCQVIVQAGTTPGMIKFEATAPNLWKGS
                     TEIITVGPGSLGSVHFDEKYRLTGEAAKPRPVDKMIGADISFLPELEARGIRFKDKGV
                     EKDAIQILEDHGFNYVRLRIFHNPALENGYSPGKGFCDLKNTLLMAKRVKAAGMKLLL
                     DFHYSDYWADPGKQYKPKAWEGLPFEALKKSLYDYTYEVMDALKKQGTAPDMVQLGNE
                     INHGIVWPEGSVSHLDSLAQLINAGTAAVKAVDPRVVMMLHVALGGQNDESVFFIDNM
                     VKRGVHFDVIGESYYPKWHGTLDDLRNNLHDLVRRYGKDVIVVEYSAKKEEVHKIAFE
                     LPGGKGKGTCIWEPLSTWEALFDWKGNSNGFLEMYDLFKKQYLEK"
BASE COUNT         1746 a         2092 c         2440 g         1969 t
ORIGIN      
        1 aagcccctcg taccagttgt aaacggcgcc ttggccgggc acgacgatgc cggaactgag
       61 tacgatgttt tcattggtac aaatttctgt atcggttacc aataaaggta tcaggggcag
      121 gttttcaatg gcgacctcca ccgtacccga caccgtgcag cccgaaatgc cgctgatggt
      181 gaccatgtaa gagcctgccc gcgccgggtc aatgttggga atgaccgggt tctnnnnnnn
      241 nnnnnnnnnn nnnnnggaca caaaaccctg cggcccgctc cactgaaaag tatagatgat
      301 gcctccctgc gctgccggcg gattggcaaa caaaaacagc gtgtcgccgc cgcaaatgcc
      361 cgagttggcc gttggctgcg gcgcttccat ttctatccga atcagcgtag aacaggtgtc
      421 ggtgttgccg gccatgtcgg tagcggtcag ggtgatgggg aaagacatgc ccgcctgatt
      481 gcaactgaat atattgggcg acaggctcag cgaggcaatg ccgcagttgt cggtgctgcc
      541 gttgtctatc agtgcggggt ctatggactg gttgctcacg ccagatggat tgacatacac
      601 gatgctgggg cggcaggcgg ccacgggccg ttgggtatcc tgtacgatga tgcgataaga
      661 acaggtgtcg ctgttgcctg cgcggtcggt ggtggtgtaa aaaatctcgg tggttcctac
      721 gacaaattca tgcgtaaccg gagcagaggc agacagcgga gtggggggca aaatggtagc
      781 tcccgtactg aaatatgcta cttccaaggc ggcgtattcc aattgcgcaa agacatagct
      841 cgcaccatcg gtatcgccgt caaaagttac ttctgacggg ttgcagggat tgatgccgtc
      901 accgggcaaa ccgggcggca cgagtatagg gttggggaca atgcgaatgt ggagttgccc
      961 gtcggaggcc cagttgttaa acaaagcttg tggtatcgtg aacgcaacct gcccggcagt
     1021 atcgcagttg gcaaagcctt gcgtcgttgt gcccagcagc gtgttgtcct ctccaaaaac
     1081 ccgaacgaaa gcgccattgg tattaaaatc gccctgtgcg atgatgctca acaccgcgtc
     1141 ttctgtggca ttggcggcca cgccggtaaa ggtgtagatt ttgggttgcg ccacccggtc
     1201 attcaggttg ggatcgtatt caaacgtgag cagcgcgttg gtgtttatgg ttggttgtat
     1261 ttgagaaaaa ggcgctccaa cactacaatt atcacttacc gaaaccggca aaggcagcgt
     1321 tacgacggcg ctgcacgagt ccaggccggc agatactgtg atgtcggcag ggcaagtcaa
     1381 tacaggcgct tcgaggtctt ccaccacaat aaagtgggaa caactgtcct gattgcctgc
     1441 gcagtcggtg gcgtagtaag tgatgaggta tgcgcccgga tcgaaaatgg nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgctgtattc gtagcgcagg ccgttcaggt
     1561 cgcgggcact gtaactgagc gtagcgcgtg ccgagccggg attgatacaa acggcattca
     1621 ccgcaaaagc gcccgacata ccggaaggaa tgttcggctc cagccggatg cgcaccacgc
     1681 catctgccgc ccaggcatcc aattgctgag gcgtaatgag gatgtccaca ttggaatcgc
     1741 cgcactgagc tggtgtgtaa gcagtcgaat cgagcaggtt gccgttttcg tcataaatga
     1801 agaaaaattc ggtagcgcct tcagcatccg ccgaaaacag gctgacgctc agcgtggccg
     1861 ggccggcggt attgagcggc tgcggcagcc cgatggggat gttgaggtct atggcgttca
     1921 caggcgtgct gcccaactga tcgggcgccg cattgctggt gatgggttgc tggtcggagg
     1981 cagtttcaga caaaataccg aacccgcaat cttccccgat gacgggcgga atgagttcta
     2041 ccaaagcgct gcactggccg gggtcggtag ggaagagcat gtcggagggg cattgcacaa
     2101 aaaacggcgg cagattgtct attacccgga atacggcagt ggtggagtcc cggttgccgc
     2161 attcgtctgt cactacaaaa atcacggtct gactgagcac tacgctgtcg gcggcagggc
     2221 aactgacggc aggcaaagca gccggatcgt tggtgccggc gttgaaagcc gaccagacca
     2281 aatcaccgga gaaggtgcag ttgtcggaag ccgtagcgcc tcctcggttt tgaatccatt
     2341 gcgtaaaagc agcctgatag tcggagttgg aggcacaaaa cacttctaag ttttgggcct
     2401 catcctgtac caccggcgct atgctgtcgc tcacaaagat gagttgaatt tgagaagcct
     2461 cattgccgca gctatcccgc accgtccagg tacggcgaat ggtgtagctg ttgatgcagg
     2521 cgcccggcag cagcacatcg ctgggcggca ggatggttcc tccagtctgg cagttgtcgg
     2581 tgatgccggt aggaaatccg gtcactgtgg ggttgtcgtt ctggtcgcag cggatagaga
     2641 tgtcggccgg cacagtaaag ctgggcggca cggtatcttt gacggtgatg acctgaattt
     2701 tgacggtaga attgttgcac acatcctgta cgcgccacag gcgttggagg gtgtagttgc
     2761 cggggcagtt gccgggcgag atttgatttt gataagaaga ggtggcgaag gcagccgagc
     2821 aattgtctgt gatattggag agattgccgg tcagccccag atcattggag tcatcggtgc
     2881 aaagaatagt aatattggca ggccgggtaa aatcgggcgc cgcatcgtcc agcactctga
     2941 tgatttgaga agcggaagac ctgttgccgc aagcgtcggt agccacccag gaacgcacca
     3001 cctgatactg gtagtggccg caatcggcag ggtttactgc gcggttgttc acctccgtaa
     3061 agacgatatc cacgtcgccg ctacagttgt cggtggccgt gaccacaggc aggggcggca
     3121 gcggctgatc gcaacgtatg gtatcggtga gcggaattcc cacgagtacc ggcttgatgg
     3181 tatcggtggc agtgagcgtt accggccagt tgagggtgtt gttgcaggcg tcggtagcgg
     3241 taaactgaat ggtgagcgta ccacaaccgg gcgggcgcgt gggcggcgcg ttattgctga
     3301 aattcaagcc cataccgatg gcgctgcaat tgtcggtggt attggacaaa aacgccaatt
     3361 gcacgccggc cagccatgcc gagtagttga tggcctcgca gggcaatatg tcattgacgg
     3421 gtacggcggt ggtgaccggc ggcgccgtat cgggcagcac gatgatgacc tgcgtgaggg
     3481 tggtcacgtt gccgtctaag tccgtagctg tccagacgcg ctcaaaagag cctccggtgc
     3541 agccattgat gctgctcggc agcgggttgt ccaccggcgc aatgagctgc ggaaacgcgg
     3601 ggtcgttgtc atcggtagct tccagttttt cgccattagg cacgtccttg agacaggcaa
     3661 aggtgagcgt gtcgggcaca ttggcggcat tggtaaaaac gggcggatcg ctgagtggcc
     3721 gagagcctgc cggcgcgccg tagagtgacg aagtgaggaa caatgccgcg agtgccgcga
     3781 ggatgacagc agtagatttg attttcatgt ggctgttttt tgactcatag taatgtttat
     3841 gggatagcat cttaaaacga agttccaaaa agcatcaatt gcccaacatt gcgcatacgg
     3901 agaaaccgcg cgccggaggc gaaagcaaga aaacgaatgg tgagatacag actgactgcc
     3961 tgtcaggcgt tgacacgttc atggaatatc ggttcttgag caaaatcggc gcaagataac
     4021 ccaatcggct cataaaatca agcacaaggc aggataaaac tcattcggcg gcatcttcta
     4081 cagcagcttc gtctttctct tttttatccc ggaacttctt gtcgtccaca ttttcattca
     4141 gataggcgtt tacccggtca attgccatcg tggtttccac ctgcccgaac tcgttgattc
     4201 tgatggaaaa tccctgcaaa ttctgcgcgg attcgtcctg ctgttgttgg tttttctttg
     4261 ccatggtttt tgtcgtttta cgttcaaggt tccgggttgg acccgcacac cgcccaatcg
     4321 cgttcctgcc cggccgtctt ttgatggcga acgccatcct gtccaagcga tgtgtttttt
     4381 ttcgcaaata tacaaaagcc aggcgtttta tcggggcgcg tgtttttttc agccgccaag
     4441 cgaggaggta gcttcgaagt gacctcctcg cttcaccgga acaaattggc acgcctcact
     4501 tctcaagata ttgcttttta aaaaggtcgt acatttccag aaaaccattc gaatttcctt
     4561 tccagtcgaa cagagcttcc caggtgctca gcggctccca gatgcaggta cctttcccct
     4621 tgccgcccgg caactcgaaa gcgattttat gtacctcctc cttttttgcc gaatactcta
     4681 ccacgattac gtccttgccg taacgacgga cgaggtcgtg caggttgttc cgcaggtcgt
     4741 cgagcgtgcc gtgccatttc gggtagtacg actcgccgat cacgtcgaaa tgcacgccac
     4801 gtttcaccat gttgtctata aaaaagaccg actcgtcatt ttgcccgcca agcgccacat
     4861 gcagcatcat caccaccctt gggtccacgg ctttcacggc ggcagtgccg gcattgatga
     4921 gttgcgccag actgtcgagg tgactcacgc tgccttccgg ccagacgatg ccgtggttga
     4981 tttcgttgcc aagctgcacc atgtcgggcg cggtgccttg ttttttcagg gcatccatta
     5041 cctcgtaggt gtagtcgtag agcgattttt tcagtgcttc gaagggcagt ccttcccagg
     5101 ctttgggttt gtattgcttg ccgggatctg cccagtagtc ggagtagtgg aagtcgagga
     5161 ggagtttcat gccggcagct ttcacccgtt tggccatgag cagcgtgttt ttcaggtcgc
     5221 aaaaaccctt gccgggcgag tagccgtttt cgagggcagg attgtggaaa atgcgcagcc
     5281 ggacgtagtt gaagccgtgg tcttccaaaa tctggatggc atctttttcc acgcccttgt
     5341 ctttgaaacg gataccccgt gcctccaatt cggggagaaa ggagatgtcg gcgccgatca
     5401 ttttgtccac cgggcggggc ttggcggctt cgccggtgag ccggtatttt tcatcgaaat
     5461 gaaccgagcc gaggctgccc ggcccaacgg tgatgatttc ggtggagcct ttccagaggt
     5521 tgggcgctgt ggcttcgaat ttgatcatgc ccggcgtcgt gcctgcttgc acgatgacct
     5581 ggcatttacc gttgaaaaga ctgcgctgcc agcgtccgtc ggcgcactta tccggttcgt
     5641 ggcaggaggg gtcgccattg cccacaccga tgattttgcc gtcgccttcg atgtgaaact
     5701 ggatgaggtt gtcggcattg ggcacttccc ggccttcccg gtcgaacacg gtcacattga
     5761 tgaccgttgc gtcctgtccg tcggccacca tcgtggtttt gtagggtgtc agcacgacgt
     5821 cgaatggggt gccggtggtt tcgaccttag cggtgagttt tttgcctttt ttaaaagcca
     5881 ctgcttctaa tttgccgggt tcgtatttca ccttccactt gaggtggctg ttgcggggca
     5941 tgtccttttt gccgaggctt ttcccgttta aaaaaagctc tacatcgtcg gcattggtgt
     6001 tgacccagac atcaagggag tcgctccggc gatccatcca gttccagtgg ggagagatgt
     6061 gcagtacgtc gttgtccgtc caccaggatt ggtagtagta gtagatgttt ttcgggaacc
     6121 cgcacatatc catgatgccg aagtgcgaat tgatgttcgg ccaggcgaag ggcgtcggtt
     6181 cgccccggta gtcgaggccg gtccatatga agccgccgag ccagaagtcg ttgtctgcgg
     6241 ccagtgtcca ccaatcttcg gcggtactgg cccaccaggg ggcgctgatg tcctgatcgg
     6301 gcacatagcc acggatgctg tcccgctcgt actgtccacg tgtggtcacg gtactgccca
     6361 tttccgtacc gatgatgggt tggtgggggt ggtcgcggtg gtagtcggcg acggcgaagt
     6421 ggcggtagtt aaagctgcgc acggggatga tttcgttgat gccctcgaag acattgggca
     6481 ggtcggcggc ataggtactt gtgcgggtcg ggtcgagttg ctgctgtttt ttcaacagcg
     6541 tttcagcaat gcgcttgccg gtcggggagg tatgaatcca gccttcctcg ttgccgatgc
     6601 tccacataaa gacacaggtg cggctgcggt cacggcgaag caggcgctcg aattggccga
     6661 ggtattcggg gctgctgttg agcaggcggg tttcatccat gaccaacatg ccgaggctgt
     6721 cgcaggcgtc gagcagttcg ggggtgggtg ggttgtggct ggtgcggtag gcattgacgc
     6781 ccatattttt cagcaaacga atgcggtagt attgcaggta gtccggcagg gcgctgccca
     6841 cacccgcatg gtcctggtgg caatttacgc cgtataattt cacgtgtttt ccgttgagaa
     6901 aaacgccatt ggttttcact tctattttgc gaaccccgaa acgcagtatt tgccggtctg
     6961 tcacttggcc gttcgacttt atatttacat gaatccggta taaatagggg ttgtccaaat
     7021 cccacaatac cggattgttt accgttagtt tttgcaatat tgattgattt tccaaaatat
     7081 tgacagacag gtttttagtc tcagtttttg caacgatttt cccctgattg tctgtcagat
     7141 aggtttcaat ggaaatatcc gattttctgt tgcctttatt ttccaatgtt gtttctacgg
     7201 aaataacagc cttcccgtct tttatttcag aatatgcaaa tatgccatcg ttcacaatat
     7261 gcgtttggtc aaaaacattg agccagacat ggcgataaat gcctgctcct tcataaaacc
     7321 aaccctcgta ttgcgttgca tctacccgca caacgagtac gttttcctcg ccgtacctga
     7381 taaaatccgt tatatcccag gccacgccca tgtagccgct ggggttgttg ccgaggtaca
     7441 tgccgttcag ccagaaatgt gcatcccgga acacgccgtc gaactggagt tcaaaacgcc
     7501 ggccctcgtc tgctgcgttc accgtgaagc gcttgcgata ccagccgatg ctcgtctccg
     7561 gaaacttgcc cccgacgggc ttgtagccgt gcgccatgac gttgaaatcg gggctgttca
     7621 cgaagggcag ttctacagcc cagtcgtgcg gcaggtcgag cgagcgccag aggctgtcgt
     7681 tgaagcgggg gtcaacggcc gtattgagcg cctcgccgga ttttttgaaa agcggttcga
     7741 tactgaagcg gaagtccttt tccgggtttt cagcgtggcc gaaagcgaat ttccagtcgg
     7801 tgtcgaagtt gaggtgccgg cggggagaaa gttcttgagc gtgcgcctgg ctgcaaaaga
     7861 aaaacagcac caggagcatt ggcagtaccg atttcatatc tcgtatattt tgacggctaa
     7921 gataaggcca acttttgggt tttaatccat ttggccttat tagctgatgc tgttttaatg
     7981 atgagccggc aagtgaggaa gtaacttcaa gttactgcct cacttcatca gaaaatgaaa
     8041 ccggaaccag cgaggtgaca tctgccgccc tgctgcaaca accggagggc ggaaggtgtc
     8101 atctcgcggc tgcgaaggga aaatctcccc gttttcatgc gacaccttcc cgcctcgcta
     8161 aggctcgtcg tttagatgcc acctgaaaac actgagccaa acatggacca agaaaagcat
     8221 aaaagacgcc aaccggacca gcggtgccgg cgattgtttt ttttgatgtt ttgctaagga
     8281 agactctgtg ccagcggaga aagcagcg
//
LOCUS       DAORLF010000199         8275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4666_length_8275_cov_2.647810, whole genome shotgun sequence.
ACCESSION   DAORLF010000199 DAORLF010000000
VERSION     DAORLF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4666_length_8275_cov_2.647810"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1408
                     /locus_tag="PK198_15215"
     CDS             293..1408
                     /locus_tag="PK198_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF40139.1"
                     /translation="MKNFLFLMLPLLAAITLTQCAPQKGTTVEGTIQNAGNLQAFMDK
                     VVIGQANSVLGKVDINGSGNFEFSFPEGLEAGVYGLRIGARRFNFVTNGHEKKIAIQG
                     DLNNLENFQGITVSGSPDTEAYLKIMQGLVAQQVSSQNIETFIDTCSNPMLGAFVAFR
                     SLSGSAAFLPLQKKALAKLQAAHPTAEITAGYTQFLSTMESQIMAQQAQELIQVGQPA
                     PDINLPNPKGRNYKLSDLKGKIVLLDFWASWCGPCRRENPNVVAVYNKYKDKGFTVFS
                     VSLDGVESRALAGPAPDQIKQVQMASKQRWIDAIQQDGLAWEYHVSDLKKWEAAPAAM
                     YGVSGIPRAFMIDRNGIITSTNVRGAEAIEAEILKLL"
     gene            1516..1755
                     /locus_tag="PK198_15220"
     CDS             1516..1755
                     /locus_tag="PK198_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40140.1"
                     /translation="MTAHTVYDHVADFISRMNPQLLLELKAPEAMSIRLEDLIDKEKE
                     GTLSSEEKDELDHYIVLERLIRLSKAHARLRIAEQ"
     gene            1776..2165
                     /locus_tag="PK198_15225"
     CDS             1776..2165
                     /locus_tag="PK198_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRF40141.1"
                     /translation="MRKAAAERAGSRCEYCLLPAVDSFYGFQIDHVVSRKHGGTTDLD
                     NLACACPDCNRSKGTDLGTLLDDSTNLIRFFNPRLDDWDTHFECAETGMIYARTNIGR
                     ATLKIFQFNHPDRIIERKLLWQLGLWP"
     gene            complement(2218..2592)
                     /locus_tag="PK198_15230"
     CDS             complement(2218..2592)
                     /locus_tag="PK198_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40142.1"
                     /translation="MIISDDKKLREVKEEFHKKFTHLKIEFYSGSHQRGQGSRQSELL
                     DAELTLGQARTQHNEGDFRIDPQMPVGEFEQKFLAKYGLNAQVFRKSGNLWMQTTATD
                     SWTLAEQNRKGGASEEAYHEKV"
     gene            complement(2683..>3088)
                     /locus_tag="PK198_15235"
     CDS             complement(2683..>3088)
                     /locus_tag="PK198_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF40143.1"
                     /translation="YSVRALQTAAAIALRLPQPPELVVTHLYELPNIFTYRLGKSEEE
                     LKAILDADREAALKDFVNNYAPEAERVRIRIERHDDADLARQISDAALQCNADLIVMG
                     AKGHSRVERLLMGSVTEKLLLVNDSIPVWVVK"
     assembly_gap    3089..3107
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3108..3551)
                     /locus_tag="PK198_15240"
     CDS             complement(<3108..3551)
                     /locus_tag="PK198_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF40144.1"
                     /translation="MVALALSAADDTTLHYVSFLAGRVPVSSAFFLHVAGRLDLYSAM
                     LEREAQAMVSNFDYHTDQARQLDKVARDRMGAGTQVQCDVREGDPLEEVLRECVDLQA
                     DLLVAGKKSGASAHGILANNLVRETPCSTLVVPQQSRIQIERIFVP"
     assembly_gap    3766..3798
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3823..7128
                     /locus_tag="PK198_15245"
     CDS             3823..7128
                     /locus_tag="PK198_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HRF40145.1"
                     /translation="MDNYRLLVEKLDQFIRKYYINQLIRGSLYSIGLILVLFLAMSLL
                     EYNFYFSTGVRKGLFYTFVGVSVVALAGWVLLPLLRYFRLGSIISHERAAQIIGSHFG
                     NVKDKLLNVLQLRRQADTVDNKELILASINQKSEEIKLVPFKSAINLGQNRKYLRYAL
                     PPLMLFLVLIFAAPSFRDSTRRLLNSNIEFLRPAPFSFVVPGDSLRVGQFEDFPLMVR
                     IEGEQLPNEVFIEVDNFQYRLTKEGPSLFSYKFVNVQKDTDFRLFSGTVRSQKFTLDA
                     LEKPNMLGFEVKLNFPSYLQRPAETLNSIGDLVLPLGTTIDWVFNAENTDDVTLLFAS
                     QEKEQKANRFSDDLFTFKRRAMQDESYKVLISNKLLPKADSINYIITVIPDLYPTIKA
                     DKYQDSTDIKLLFFAGDASDDHGLSHLTFNYRLKKAGKQQGELVTRSLKRPGVKQTNF
                     DYTFDMAKLGLEPGDEVTYYFEIFDNDGVNGPKSSRTNLMVFSMPTVEEFKAMAEANN
                     EQIKDDLLKALEESRKLQEELKKMREKMLQEKDLTWQNRKELEKMLERQKQLEKQIEQ
                     AQQAFQENLKNQQEYSKTEQNILDKQEKLEQLFEEVMSDEMKELMQQIEELMQQLEKE
                     NALEMMENFQFNNEDLEKELDRMLELFKQLEVEKAMEDAVKDLEKLAEEQEKLAEQTE
                     KNQKSQEELKKEQEKINEKFEDIKKNVEEAHKKNEELEKPQDLGDQEEEMEDIKEDLE
                     NSEQELQEKQNSKASKSQKKASQKMKNMANSMSMQMQQAEMQQTEEDMKSLRQLLENL
                     VGLSFNQESLIKDFNKTPINTPKYVQLTQEQFRIKDDFKLVQDSLQALAKRVFQIESF
                     ITEKVSDINSNFRSTLADLEERRKPQAADHQQRAMKNINDLALMLSETLNQMQQQMAG
                     MMSGNQMCKNPGGSGRKPSDRITPGQQEMKESLEQMKGRMDKGQKPGSKEFAQMAAKQ
                     AALRKALREKQQELQQRGQGSPGLQEAIDQMDKVETDLVNKKLTNETLRRQQDILTRL
                     LEHDKAERERGLDEQRKAEVAREKDRKMPPSLEEYLKKREAEVEMYKTVSPALKPYYK
                     QLVEEYFKSVKSN"
     gene            7289..7702
                     /locus_tag="PK198_15250"
     CDS             7289..7702
                     /locus_tag="PK198_15250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF40146.1"
                     /translation="MEKRVQQVMLKLPSDPRNIELVESFVQKVAEKYRLSPDQFGNLR
                     LSVTEAVNNAMIHGNGQDESKNVCIELHKRKDAIAVRISDEGRGFDYNNVPDPTLPEN
                     VCKCGGRGVFLMRNCSDRIDFRNNGSTVEMQFKLW"
     gene            7696..8181
                     /gene="ybeY"
                     /locus_tag="PK198_15255"
     CDS             7696..8181
                     /gene="ybeY"
                     /locus_tag="PK198_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRF40147.1"
                     /translation="MVEPYNEEPVKGAPYQDEDEERIQFHFEDTEFELLYPARLRQWI
                     EQVIARESCILQTVSFIFCSDDYLYDLNVQYLSHDTLTDVITFPYLKPPHIEGDIFIS
                     ADRIRDNAGEFGVSFEQELHRVMIHGILHLCGYSDKTDADKAAMTQKEDEALGVLRDL
                     S"
BASE COUNT         2276 a         2159 c         2013 g         1775 t
ORIGIN      
        1 aaaattagaa aacaaggggc aggtgaaaaa ttaacatagg aaacgagaca agataaatat
       61 tggatttata ctcgatagcc agtggtttgc gaatttagat ttttgcaaaa accactggct
      121 atcagttatc ccgatgaaaa atggtttata ttttttcgca aaccattttt catcgggtat
      181 actacagaaa atgtactgtt ataactcagc agtagcgccc gccccaacca ttttcagcct
      241 tatcgtgttg atgaagcact gtaaacaata ccattaactt tcttaaaaaa atatgaaaaa
      301 ctttctcttt ctcatgctgc cgctccttgc ggccatcacc ctcacacagt gcgcgccgca
      361 gaagggcaca accgtagaag gcaccattca aaacgccggc aatttgcagg cattcatgga
      421 caaagtcgtc atcgggcagg ccaactctgt gctgggcaaa gtggacatca acggttcggg
      481 caattttgag ttctccttcc ctgaagggct ggaggctggt gtgtacggcc tgcgcatcgg
      541 agcgcgtcgt ttcaattttg taaccaatgg ccatgagaaa aaaattgcca tccagggcga
      601 cctgaacaat ctggaaaact tccagggcat caccgtcagc ggctcccccg atacggaggc
      661 atatctcaaa atcatgcagg gcttagtggc gcagcaggtt tcgtctcaaa atattgagac
      721 ctttatagac acttgctcca atcccatgct cggcgccttt gtggcattcc gttccctcag
      781 cggcagcgct gctttcctgc ctttgcaaaa aaaggctttg gccaaattgc aggccgcaca
      841 cccgactgct gaaatcaccg ccggctacac gcagttcctc tctacgatgg agtctcagat
      901 catggcgcag caggcccagg aattgattca ggtgggacaa cccgcgcccg acatcaacct
      961 gcccaacccc aagggcagaa actacaagct ctccgacctg aagggcaaaa ttgtactcct
     1021 cgacttctgg gcaagctggt gcggcccctg ccgccgggaa aaccccaacg tggtggccgt
     1081 ttacaacaaa tacaaagaca aaggctttac ggtgttcagc gtttcgctcg acggcgtgga
     1141 gagccgcgca ttggcaggcc cggcgcccga tcagatcaag caggtgcaga tggcatccaa
     1201 acagcgctgg atagatgcca tccaacagga cggacttgcc tgggaatacc acgtcagcga
     1261 cctgaaaaaa tgggaagcgg cgccggcggc gatgtacgga gtgtcgggta ttccgcgcgc
     1321 ctttatgatt gaccgcaacg gcatcatcac ctccaccaat gtgcgtggcg ccgaagccat
     1381 tgaggctgag attttgaagt tgttgtaata aaagagaagc caagcggata catttgagtg
     1441 tgtccgcttg gcttggtctt ggggcaagaa ctgttttcaa ttattgtctt tcatcatatc
     1501 taacaactcc tccgtatgac agcacatact gtttatgacc atgtagccga cttcatttcc
     1561 agaatgaacc ctcaattgct actggaactc aaagcccccg aagcaatgag tattcgctta
     1621 gaggatttga tcgacaaaga gaaagaggga actcttagtt cggaggaaaa agacgaactg
     1681 gatcactata tcgtgcttga acgcctgatt cgtttgtcca aagcgcatgc acggttgcgg
     1741 attgcagagc aatgagcaga aaaatccctg atgcaatgag aaaagcagcg gccgaacgag
     1801 caggaagccg ttgtgaatat tgtctattac cggcagtaga ctctttctat gggtttcaaa
     1861 tagaccatgt agtgagccga aaacacgggg gaactaccga tcttgacaac cttgcctgcg
     1921 cctgcccaga ctgcaataga agtaagggga cagaccttgg caccctactt gacgattcaa
     1981 cgaatctcat acgattcttc aatcctcgcc tggatgactg ggacacccac tttgagtgcg
     2041 ctgaaaccgg tatgatatat gccagaacca atatcggaag agctactttg aaaattttcc
     2101 aattcaatca tcctgataga attattgaaa ggaaactgct gtggcagttg gggctatggc
     2161 cttagatttc atgtatagcc taaaaccctc agccgctaca tctcacctcc tcctctatca
     2221 cactttttca tgataagcct cttccgatgc gccgcccttg cggttttgtt cggccaaagt
     2281 ccaggaatcg gtagcggtag tttgcatcca gagattgcct gacttgcgga aaacctgtgc
     2341 gttgaggccg tatttggcta agaatttttg ttcaaattcc cctaccggca tttgcgggtc
     2401 tatgcgaaaa tcgccctcgt tgtgttgggt gcgcgcctgt ccgagggtca gttccgcgtc
     2461 caatagttcg ctttgacgag agccttgacc gcgttggtga ctgcctgagt agaactcaat
     2521 tttgaggtgg gtgaattttt tgtggaattc ctcttttacc tctctgagct ttttatcgtc
     2581 ggaaatgatc atggcgtgtt tggttgaatg aaacaaaaaa agaccgtcaa agcgagaaga
     2641 acccaacctt gacagtcctt cgaaaactaa cctcactaat ttttatttaa ctacccaaac
     2701 gggaatggaa tcattgacga gcagcaactt ttcagtgacg ctgcccatca acaggcgttc
     2761 cacccgcgaa tggcctttgg cgcccatcac gatgaggtcg gcgttgcatt gcagggcggc
     2821 atcgcttatc tggcgggcca aatccgcgtc gtcgtgtcgt tctatgcgga tgcggacgcg
     2881 ttcggcttcc ggcgcgtaat tgttgacaaa atctttgagt gccgcttccc ggtctgcatc
     2941 aagaatcgcc ttgagttcct cttccgattt ccccagccga taagtgaaaa tattgggcaa
     3001 ctcgtagagg tgcgttacta ccaattcggg cggttgcggc aggcgcaagg ctatggctgc
     3061 agcggtttgc aaagcgcgta ccgagtagnn nnnnnnnnnn nnnnnnnagg cacgaaaatg
     3121 cgctctatct gaatgcgcga ctgctgcggt acgaccaagg tactgcacgg cgtttcccgc
     3181 actaagttat tggctaagat gccatgtgcc gaagccccgc tttttttgcc tgccaccaat
     3241 aaatctgcct gtaaatcaac gcattcccga agtacctcct ccaaaggatc accttcgcgg
     3301 acatcacatt gtacctgtgt gccggccccc attcggtcgc gggcaacctt gtccaattgc
     3361 cgggcctgat cggtatggta atcaaaattg ctgaccatcg cctgcgcttc gcgttccaac
     3421 atggcgctgt acaaatccag acgaccggca acgtgtaaaa agaaggcaga tgaaacgggc
     3481 acccggccgg ccagaaacga cacataatgg agggtggtgt catcggcggc agaaagggcc
     3541 agcgccacca tcaatttgtc gtacttcgag cggcgaaact ttggccccgg aacgggtgat
     3601 atgggcagct attttattca tctttcactg cgttttccaa gcgcttcatg ccaaaattac
     3661 ccctttctat ctctcccaaa gatgacaatt atcaaaacag cgtttgactt ttgtcaatac
     3721 atttgccctg tctgaggacg cgttgtgtca accttttgcg tgctcnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnngt tatacaaaag gaatcgccca aaatggataa ctaccggctg
     3841 ttggttgaaa agctcgacca attcattcgc aagtattaca tcaatcagct cattcggggc
     3901 agcctgtaca gcattggcct catattggtg ctgtttctgg ctatgtcctt gctggagtac
     3961 aatttctact tcagtacagg ggtgcgcaag ggcttgtttt acaccttcgt gggcgtcagc
     4021 gtagttgcgt tggccggctg ggtattgctg ccgctgctgc gttattttcg cttgggcagc
     4081 atcatcagcc atgagcgggc cgcacagatc atcggcagcc atttcggcaa tgtaaaagac
     4141 aagctactca acgtgttgca actccgccgc caggccgata ccgttgacaa caaagaactt
     4201 atcttggcca gtatcaacca gaaatcggag gaaatcaagc tcgtgccgtt caaatcggcc
     4261 atcaatttgg gccaaaatcg caaatacctg cgctacgccc tgccgccgct catgctgttt
     4321 ctggtactga tttttgcagc gcccagcttc cgcgacagta ccaggcgcct gctcaacagc
     4381 aacatagagt tcctccgacc ggcgccgttt tcctttgtgg tacccggcga cagcctccgc
     4441 gtggggcagt ttgaggactt cccgctgatg gtgagaattg aaggcgagca attgcccaac
     4501 gaggtcttca tagaagtgga caatttccag tatcgcctta ccaaagaagg ccccagcctg
     4561 ttcagctaca aatttgtcaa cgtacaaaaa gacaccgatt tccgcctgtt ttccggcacg
     4621 gtacgttctc aaaaatttac gctcgacgca ttggaaaaac ccaatatgct gggctttgaa
     4681 gtcaaactta atttcccctc ctatctccag cgccccgctg aaaccctcaa cagcatcggc
     4741 gatctggtgc tgcccctcgg cactaccatt gactgggtgt tcaatgcgga aaataccgac
     4801 gacgtgaccc tgctctttgc gtcgcaggaa aaggaacaga aggccaaccg tttttcggac
     4861 gacctgttca ccttcaaacg ccgggccatg caggatgaaa gctacaaggt gcttatttcc
     4921 aataagttgc ttcccaaagc cgactccatc aactacatca tcaccgtcat tcccgatctc
     4981 tatcccacca tcaaagccga taaatatcag gacagcaccg acatcaaact gctctttttt
     5041 gccggcgatg cttccgacga tcacggtctg tcgcacctca ccttcaacta ccgcctcaaa
     5101 aaggccggca agcagcaggg cgaactcgtg acgcgctctc tcaaacgccc cggcgtcaag
     5161 cagaccaact tcgattacac cttcgacatg gccaagctcg gcctcgaacc cggcgacgaa
     5221 gtgacgtatt actttgaaat tttcgacaac gacggcgtaa acggccctaa atcgagccgc
     5281 accaacctca tggttttttc catgcctacg gtagaggaat tcaaggcgat ggcagaagcc
     5341 aacaacgaac agatcaaaga cgacctgctc aaagcgctcg aagaatcgcg caaacttcag
     5401 gaagaactta agaagatgcg cgaaaaaatg cttcaggaaa aagacctcac ctggcaaaac
     5461 cgcaaggagt tggagaaaat gctcgaacgc caaaaacagt tggaaaaaca gattgagcag
     5521 gcgcagcaag cctttcagga aaacctgaaa aaccaacagg agtacagcaa aaccgagcaa
     5581 aacatcttag acaaacagga gaaattggag caacttttcg aagaagtcat gagcgacgaa
     5641 atgaaagaac tcatgcagca gattgaggag ttgatgcaac aattggaaaa agaaaacgcg
     5701 ctggaaatga tggagaattt ccagttcaac aacgaagact tggaaaaaga actcgaccgc
     5761 atgctcgaac tcttcaagca gttggaggtg gaaaaagcga tggaggatgc cgtaaaagac
     5821 ctggaaaaat tggcagaaga acaggaaaaa ttggcggagc aaaccgagaa aaaccagaaa
     5881 tcgcaggagg aactgaaaaa agagcaggag aagatcaacg agaaattcga ggatatcaaa
     5941 aagaacgtag aagaggcgca caagaaaaac gaggagttgg aaaaaccaca ggacctcggc
     6001 gatcaggaag aggaaatgga agatatcaag gaagacctcg aaaacagcga gcaggaattg
     6061 caggaaaaac aaaacagcaa ggcttccaag tcgcagaaaa aggcttcgca gaaaatgaaa
     6121 aacatggcca acagcatgtc catgcaaatg cagcaggccg aaatgcagca aaccgaggaa
     6181 gacatgaagt cgctgcggca attgctggaa aacttggtgg ggctttcctt caatcaggaa
     6241 tcgctcatca aggatttcaa caaaacgccc atcaatacgc ccaaatatgt gcaattgacg
     6301 caggagcaat tcagaatcaa agacgatttt aaactcgtgc aagacagcct gcaggcattg
     6361 gccaagcgtg tttttcagat cgaatctttc atcaccgaaa aagtgtcgga catcaactcc
     6421 aacttccgca gcacgctggc cgatttggag gaacgccgca aaccgcaggc tgccgatcat
     6481 cagcagcgcg ccatgaaaaa catcaacgac ttggccctca tgctcagcga gaccttgaat
     6541 cagatgcaac agcaaatggc gggcatgatg tcgggcaacc agatgtgcaa aaatccggga
     6601 ggttcgggcc gcaaaccctc cgaccgcatc acgccgggac agcaggaaat gaaagaatcg
     6661 ctggagcaaa tgaaaggccg catggataaa ggccagaagc ccggcagcaa ggaatttgcc
     6721 caaatggccg ccaaacaagc cgccctgcgc aaagccctgc gcgaaaaaca acaggaattg
     6781 cagcaacgcg ggcagggcag ccctggattg caagaggcca tagaccagat ggacaaggta
     6841 gaaaccgact tggtgaataa aaaactcacc aacgaaaccc tccgccgcca acaggacatc
     6901 ctcacccgcc tgctggagca cgacaaggcc gaacgcgaac gcggcctcga cgagcagcgc
     6961 aaagcggaag tcgcccgcga aaaagaccgc aaaatgccgc cctcgctgga agaatacctc
     7021 aaaaaacgag aggctgaagt ggaaatgtac aagacggtat cgcccgcgct caaaccgtac
     7081 tacaaacagt tggtggagga gtatttcaaa tccgtcaagt cgaattaaaa cgcttaccgg
     7141 gcggcttggg aggagtatat ctgccaagcc gcccgtcttt tttttaaaca ctcttatttg
     7201 acaatcagca tattagcgta ttgccgtatc aatacagcca cctatacccc ttgcgccgta
     7261 tcggcttttg tcttaaattg cgtgttctat ggaaaaacga gtgcagcagg tcatgttgaa
     7321 actaccttcc gatccacgaa acattgaact cgtggaatct ttcgtccaaa aagttgctga
     7381 aaaataccgg ctcagcccgg accaattcgg caatcttcgc ctcagcgtca cagaggccgt
     7441 caacaatgcc atgattcacg gcaacgggca agacgaatcc aaaaacgtct gtatcgaatt
     7501 gcacaagcgc aaagacgcta ttgctgtgcg catctccgac gaggggcgcg gcttcgatta
     7561 taacaacgtg cccgacccta ctctgcctga aaatgtgtgc aaatgcggcg ggcgcggcgt
     7621 tttcctcatg cgcaattgct ccgaccgcat tgacttccgc aacaacggca gcaccgtgga
     7681 aatgcagttc aaactatggt agaaccttac aacgaagagc ctgtcaaagg tgcgccatat
     7741 caggacgagg acgaagagcg catccagttt catttcgagg atacggaatt tgagttgttg
     7801 tatcccgccc ggttgaggca gtggatagag caggtcattg cccgcgaatc ttgcattttg
     7861 caaacggtct ccttcatctt ttgcagcgac gactacctct acgacctcaa cgtgcaatac
     7921 ctcagtcatg ataccctcac cgacgttatc accttcccgt acctgaagcc gccccacatc
     7981 gaaggcgata tcttcatcag cgccgaccgc atccgcgaca atgccggcga gttcggcgtc
     8041 agctttgagc aggaactcca ccgcgtgatg attcacggca tcctccacct gtgcgggtat
     8101 tccgacaaaa cggatgccga caaggccgcc atgacgcaaa aagaggacga ggcgctgggt
     8161 gtgttgcggg acttgtcttg attttgcgcg tagtaccgtg gcacctctcc gagcagcgaa
     8221 aatcataaaa atcataaaga tcagcgtcgg aaaaatcctc tccgggcagc ggaaa
//
LOCUS       DAORLF010000200         8259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4681_length_8259_cov_2.035471, whole genome shotgun sequence.
ACCESSION   DAORLF010000200 DAORLF010000000
VERSION     DAORLF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_8259_cov_2.035471"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>764
                     /locus_tag="PK198_15260"
     CDS             <1..>764
                     /locus_tag="PK198_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRF40148.1"
                     /translation="TLISPQELAAAEIARLVQQCSFTDIFEFEEGKIALAGITLDDSS
                     RLLNVPLCSFGQYCTDDSVRPMAILRGHQTIIPHGDTVLRRGDHVYFITKRENIDELN
                     QLVGKTKVRLKNIMIVGGTPLALATAKRLEADYNVTLIEKSKDVCKELADELRNTLII
                     HGDAGNVTLLEEEGIHRMDAFIALSPNSETNIIASLTAKNFGVYKTIAQVENKEYIHI
                     SQNIGVDTLINKKLIAANNIFRFVRKGHVEAITSLH"
     assembly_gap    765..789
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <790..996
                     /locus_tag="PK198_15265"
     CDS             <790..996
                     /locus_tag="PK198_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRF40149.1"
                     /translation="IEYVITKANQLTKKSLKELHFPKTALIGGVIRGEESLIPTGDFR
                     LEVDDKVIVFAMPDAIGKLDQLFR"
     gene            1012..2466
                     /locus_tag="PK198_15270"
     CDS             1012..2466
                     /locus_tag="PK198_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRF40150.1"
                     /translation="MINIRPILRFSGVLLVLLGVFMLSALPVSWWFGGQDFQALLWSA
                     LICIGIGLPAWWWLRKDAKGNIGKREGYLIVAGGWLLMSVFGMLPYLLSGEIPEWYNA
                     FFETSSGITTTGASVLTDIEAVTPGILYWRSLTHWIGGMGIIVLTVALMPLLGIGGIE
                     LFVAESPGPTSDKLHPRIRETAKRLWLIYVGFTIVLALLLWAGGMTPFDAVNHSFATV
                     ATGGFSTKNASMAYYESPFLHYVTIFFMIAAGANFSIHYHLLKRHFSTVFKNEEFRTY
                     LLIIAAATLVIAVSLVWHFDYGVEKAFRDSLFHVVSVITTTGFITADYSQWGPGVVMI
                     IFLLLFVGGCAGSTGGGIKIIRHLVFIKNSWLEFKRILHPRAIVPLKVNGETVAPRIM
                     THIIIFLLLYLLLTLVGSLALSVMGIDFATAVGATATSLGNVGPAIGDVGPVDNFAWM
                     PPAAKVWLGLLMIMGRLEIFTILVLFTPYFWKSN"
     assembly_gap    2507..2543
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2814..2865
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3036..3977)
                     /locus_tag="PK198_15275"
     CDS             complement(3036..3977)
                     /locus_tag="PK198_15275"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HRF40151.1"
                     /translation="MALKRLEEFRLYYNQTIQPELLRMERERLRMVTFMAASLALMAG
                     VIILELYIDVLVLTLMLSIPLGIYVIFLWRRMEEFRNRFKPRIVRLILDFIDDGLNFD
                     PSNPLIYTPDGFIGKERFVASRLFDTPAHEYKGEDKIEGKVGEMFFQLSELRVGEISI
                     LRNRLDAVFSGVFLHAVFNEPTIGAIVVWPRSRQQFLTNAVRAFTFEGGLSKDDEILN
                     TEFRSIFMTYATEDTPVAHILSPPMQEALVEFTKTHRKDLYISFLNRDIHLGLWEDKD
                     LLEPYILRSNLSFDKIREFFEDIHSLLHLVEVFDQTH"
     gene            complement(3987..>5282)
                     /locus_tag="PK198_15280"
     CDS             complement(3987..>5282)
                     /locus_tag="PK198_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF40152.1"
                     /translation="ASDLIVFVFEAKNPYRQSAWEFFEYIHQDWQKKVIFVLQQKDLM
                     EPADLEVNFNGVAQQAEKKGITAPNVFAVSAKQEQDGRIEESGFGPLRTYIRAHITGG
                     KAPLLKLQNSAAVARNILERIRAGVQLRSNQWEADVAFRTDIHDTLEQQQTKSYKHVD
                     VLVENLLAGFDRITRQKRDELSEGLSFFALLKRSLAGLFSKKTSPQEWLGAFAQSLEQ
                     DLNTEMQRKLNEGVADLSDSIQQMAKIIDLKIRNSQTILRNDHELFSSIAERRANVLR
                     DLQDTFSTFLAKTENFAQRDIFPDRSSISPNIATGSGLAVVGIILAAVTQGMVFDITG
                     GLLTTVGLLFAGVTAGVKRKRILEGFQTEVDKGRSLLNTEVSEKLKSYISQLKSRIED
                     NFSRFDQMLETEESHIRHLETQQNGIAQRLDETDSQLKS"
     assembly_gap    5283..5313
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5314..5715)
                     /locus_tag="PK198_15285"
     CDS             complement(<5314..5715)
                     /locus_tag="PK198_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRF40153.1"
                     /translation="MSQLISRDVQIYRAQVEEIIKDLHELVIQTGNEDLGRTVSDLRN
                     RINDPFMFVIVGEVKAGKSSFINALLSTGKEIAKVAPQPMTDTIQQIVFGEQEEAVTV
                     NPFLKKIFLPVDILREIAIVDTPGTNTIVEHH"
     gene            complement(5727..>6241)
                     /locus_tag="PK198_15290"
     CDS             complement(5727..>6241)
                     /locus_tag="PK198_15290"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRF40154.1"
                     /translation="AVATAGGSLVLSVPLLSGKYLQPLREVKIAYHEPWQRTHWRSIH
                     DAYRKSPFFDFYAEPLEAWYRQQPEYLVEWNTGLLRILLKALRLPVAFETTESYAPDA
                     PENYRDLRDAFAFKPRLQRPDPLYRDVRYVQVFEDRLGFLPNLSILDLLFCTGPEAAS
                     ILRRSVITDN"
     assembly_gap    6242..6268
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6391..7047)
                     /locus_tag="PK198_15295"
     CDS             complement(6391..7047)
                     /locus_tag="PK198_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF40155.1"
                     /translation="MQSTQPKTILLADAQFLVRAGLRCILAEQTEVQIVGEAATEEEL
                     FQQLQALRPDMLIIDYNSPKRFSREVIALVSGLYPTLRILAISDDSDKNSILRVLDHG
                     VLSFLTKSCGEQEVSDAVRATVRGDKFFCSKILDVLLEKSLGRTTATCAPSPLSAREI
                     EVVQLVVKGYIARQIADMLHLSPHTVYTHRKNIMKKLQLQSPAELTLYALQNGIIETT
                     "
     gene            complement(7103..7687)
                     /locus_tag="PK198_15300"
     CDS             complement(7103..7687)
                     /locus_tag="PK198_15300"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF40156.1"
                     /translation="MHDLSATYWNQRYENEHTPWDIGYASPPLLEYIKRLKDKQIRIL
                     IPGAGRAYEAIFLYRAGFEQVWVCDWAPAAFGHLLEHCPEFPRERLLAADFFELDMEF
                     DLMLEQTFFCAIDRAQRQAYARQAAHLLRGGGLLAGVLFAQEFEQEGPPFGGMAAEYQ
                     NCFEPYFEILIMEISSHSIRPRAGRELFVEMRAR"
     gene            complement(7753..>7988)
                     /locus_tag="PK198_15305"
     CDS             complement(7753..>7988)
                     /locus_tag="PK198_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40157.1"
                     /translation="LNSVKNMLRTFVICCAVDLLRSNLLRSLSANEEGPRLWRGAQFI
                     LLMERRKLLRSLSAERNLEPGTSRRWAVISGRC"
     assembly_gap    7989..8028
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1830 a         1955 c         2189 g         2073 t
ORIGIN      
        1 atacgctcat ctctccgcag gaactggcgg cagcggaaat tgcgcgattg gtgcagcagt
       61 gttcgtttac cgatattttt gaatttgagg agggaaaaat tgcattagcc ggcatcacat
      121 tggacgattc ttcgcggctg ctcaatgtgc cgctgtgcag cttcggccag tattgtaccg
      181 atgacagtgt gcggccgatg gccattttgc gggggcacca aacgatcatt ccgcatggcg
      241 atacggtgct gcggcgtggc gatcatgttt actttattac caaaagagag aacatagacg
      301 agctcaatca attggtgggg aaaaccaaag tgcgcctcaa aaacatcatg attgtggggg
      361 gcacgccgct ggcactggct actgccaaac gtttggaggc cgactacaac gtgacgctca
      421 ttgaaaagag caaggatgtg tgcaaagaat tggcggatga gttgcgtaac acactcatca
      481 ttcatggcga tgccggcaat gtaacgctgc tggaggagga gggcattcac cggatggacg
      541 catttattgc tctttctccc aattcagaaa ccaacatcat cgccagtctg acggccaaaa
      601 acttcggcgt ttacaaaacc attgctcagg tagaaaacaa agagtacatc cacatttcgc
      661 aaaacatagg cgtggacacg ctcatcaata aaaaactcat agcggccaac aacatcttcc
      721 gcttcgtgcg caaggggcat gtggaggcca ttaccagcct gcacnnnnnn nnnnnnnnnn
      781 nnnnnnnnna tagagtacgt catcaccaag gctaatcaac tgacgaaaaa gagcctcaaa
      841 gagctgcact ttcccaaaac ggccctcatt ggcggcgtta tccggggcga agaaagcctg
      901 attcccaccg gcgatttcag gctcgaagtg gacgataagg tcatcgtttt tgccatgccc
      961 gacgctatcg gtaaattgga tcaattattt cgctaaaccg cacatcaaca aatgattaac
     1021 atcaggccta tccttcgttt ttccggtgtt ttgctggtgc tgttgggcgt gtttatgctc
     1081 tctgcattgc cggtttcgtg gtggttcggc ggccaggatt ttcaggcact tttgtggtcg
     1141 gcgctcatct gtatcggtat agggttgccg gcctggtggt ggttgcgcaa agacgccaag
     1201 ggcaatatcg gcaagcgcga gggctatctc attgtggcgg gcggctggtt gctgatgtcg
     1261 gttttcggca tgttgcctta tttgttgagc ggagagattc ctgagtggta caatgctttt
     1321 tttgaaacat catccggtat caccactacc ggggccagtg tacttacgga tatagaggct
     1381 gttacacctg gtattttgta ttggcgcagc ctcacgcact ggatcggagg catgggtatt
     1441 attgtgctta cggtagcgct tatgcctttg ctgggtattg gaggcataga gttgtttgtt
     1501 gctgagtcgc cgggacctac ttccgacaaa ctgcatcccc gcatcagaga aacggccaag
     1561 cgattgtggc tcatttatgt ggggttcacc atagtgctgg ctctgttgct ttgggcgggt
     1621 ggaatgactc cttttgatgc ggtcaatcat tcgttcgcta cggttgctac gggtggattt
     1681 tcgaccaaaa atgccagtat ggcttattat gagagtccgt ttttgcacta tgtaaccatc
     1741 ttctttatga tagcggccgg cgctaatttc agcatacact accacttgct caagcggcat
     1801 ttcagcacag ttttcaaaaa tgaagaattc agaacctacc tgctcatcat tgcggccgcc
     1861 actttagtca ttgccgtgtc attggtctgg cattttgatt atggcgtgga aaaagccttc
     1921 cgcgacagcc tgtttcatgt ggtgtctgtt attacgacta ccgggttcat tacggccgat
     1981 tattcccaat gggggcctgg cgtagtgatg atcatttttt tgctgttgtt tgtggggggc
     2041 tgcgccggct ctaccggcgg aggtatcaaa atcatccggc atttggtatt tatcaaaaac
     2101 tcctggctgg agttcaaacg cattttgcac ccgcgagcca ttgttccgct taaagtcaac
     2161 ggcgaaacag tggcgccgcg catcatgacg cacatcatca ttttcctgct gttgtatctg
     2221 ttgctcacct tggtggggag tttggcgctg tcggtgatgg gcattgattt tgctacggcg
     2281 gtgggagcta cggctacctc tttgggcaat gtaggaccgg ctattggaga cgtggggccg
     2341 gtggataatt ttgcgtggat gccgcctgcg gccaaagtat ggctggggct gttgatgatt
     2401 atggggcgtt tggagatatt cacgattttg gtgctgttca caccgtattt ctggaagtcg
     2461 aactgaggaa taaaaaaacc gcacaagtgc tttaggagta gttgacnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnncagcgta gttcggtaat ccccttgccg ttgcttccga
     2581 ggaagctccg agtctccgtt gccggagttg aggcccgccc ttgccattcc gagactgacc
     2641 aatacgttgt ttgaatttgt agggacaaca cttatgggca cttgcgcggt aagaaaccga
     2701 ctttctcggc ttcggtttca aaggtaaggg ctttggccgg aaaatgcaag cagaggcggc
     2761 gtgttttttt ttcaatggat gttttttatc tttgctacac tatggccgac gaannnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngataa gatattcatc
     2881 agcctgtttg atgtatcagt tgaacagata gagcaactga gggatggtat gttgcaggct
     2941 gcgttgatgg tgcaacgtca gcgtttggca gaggctgaag tcaaccggat tttgaaagaa
     3001 cacggtatgt tgtagcccag ccggcaggac tgtgctcaat gcgtctgatc aaatacctcc
     3061 accaaatgca ggaggctgtg aatgtcctca aaaaactccc ggattttgtc aaaactaaga
     3121 ttggaacgca ggatgtaagg ttccagcagg tctttgtctt cccacagccc cagatgaatg
     3181 tctcgattga gaaaagaaat ataaaggtct ttgcgatgcg ttttcgtaaa ctccaccagt
     3241 gcttcctgca tggggggcga aaggatgtgc gccacgggag tgtcctctgt ggcgtaagtc
     3301 ataaaaatgc tcctgaattc ggtattgaga atttcatcgt ctttgctcaa acctccttca
     3361 aaggtgaaag cgcggaccgc attggtcaga aattgctgcc ggctgcgggg ccataccacg
     3421 atggcgccga tggtaggctc gttgaaaacg gcatgtaaaa aaacgccgct gaacaccgca
     3481 tcaagacgat tgcgcagtat ggaaatttcg cctacccgca gttcgctcaa ttgaaaaaac
     3541 atctcgccca cttttccctc tattttgtct tcgcctttgt actcgtgggc gggtgtatca
     3601 aacaggcggg aggccacgaa gcgttctttg ccgataaagc catccggggt gtatatcagt
     3661 ggattagatg ggtcgaaatt gaggccgtcg tctataaaat ccaaaataag gcgcacgatg
     3721 cggggcttga aacgattgcg aaattcctcc atgcgccgcc aaagaaaaat cacataaatg
     3781 cccagaggaa tagacagcat cagcgtgagt accagcacgt ctatgtacaa ctccaaaata
     3841 ataaccccgg ccatgagcgc caacgacgcg gccataaacg tcaccatgcg caggcgttcg
     3901 cgttccatgc gcaacaactc cggctgaatg gtctgattat agtacagccg gaattcttcc
     3961 aatcgtttca gcgccatttg tttgagttat gatttgagtt ggctgtctgt ctcatccaag
     4021 cgttgggcaa tgccgttttg ctgtgtttcg aggtggcgaa tatggctttc ctcggtttcc
     4081 agcatttggt cgaagcggga gaaattatct tctatacggc tcttgagttg cgagatgtac
     4141 gacttcagtt tttcgctcac ttcggtattc aggaggctgc gccctttgtc cacttcggtt
     4201 tggaagcctt ccagaatgcg tttgcgtttc acgccggctg tgacgcccgc aaagagcagc
     4261 cccacggtgg tgagcaggcc gccggtgata tcgaaaacca tgccctgcgt gacggcggcg
     4321 aggatgatgc ccactacagc caggccgcta cctgtggcaa tgttgggcga tatggagctg
     4381 cggtcgggga agatgtcgcg ttgagcgaag ttttcggttt tggccaaaaa agtagaaaag
     4441 gtatcctgca agtcgcgcag cacattggcg cgcctttcgg caatgctgct gaacagctcg
     4501 tggtcgttgc ggaggatggt ctggctgttt ctgattttga ggtctatgat tttggccatt
     4561 tgctggatgc tgtcggacag atcggccacg ccctcgttga gtttgcgctg catttctgtg
     4621 ttgaggtcct gctccagcga ttgcgcgaaa gcgcccaacc attcctgcgg ggaggttttt
     4681 ttggaaaaca gaccggcgag cgaacgtttc agcagggcaa agaaagaaag cccctcgctg
     4741 agttcgtctc gtttttggcg ggtgatgcgg tcaaagccgg ccagaagatt ttcaaccaac
     4801 acatccacat gtttgtacga tttggtttgc tgttgttcca gcgtgtcgtg aatgtcggta
     4861 cgaaaagcta catctgcctc ccactggttg ctgcgaagtt gcacgccggc tcgaatgcgt
     4921 tcgaggatgt tgcgggctac ggcggcgctg ttttgcagct tgagcagggg ggcttttccg
     4981 ccggtgatgt gcgcgcggat gtaggtgcgc agcggcccga aaccactttc ttctatgcgg
     5041 ccgtcttgct cctgtttggc cgaaacggca aagacattgg gtgctgtaat gccttttttt
     5101 tcagcctgtt gcgccacgcc gttgaaattg acttccagat cggcgggttc catgaggtct
     5161 ttttgttgca acacaaaaat gaccttcttt tgccagtcct gatggatgta ttcaaagaac
     5221 tcccaagccg actgccggta gggatttttg gcctcaaaca caaaaacgat gaggtcggag
     5281 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatggtgc tccacgatgg tgttcgtgcc
     5341 gggcgtgtcc acgatggcaa tttcgcgaag aatgtccaca ggcaggaaga tttttttcaa
     5401 aaacggattg acggtcacgg cttcttcctg ctcgccgaag acgatctgct ggatggtgtc
     5461 ggtcatgggc tgaggcgcca ctttggcaat ttcctttccg gttgacagca gggcgttgat
     5521 aaaactgctt ttgccggctt tgacctcgcc cacaatgaca aacatgaagg ggtcgttgat
     5581 gcgattgcgc aggtcgctga cggtgcgtcc caggtcttcg ttgcccgttt gaatcaccaa
     5641 ttcgtgcagg tctttgatga tttcttccac ttgtgcgcgg tatatctgca catcgcggct
     5701 gatgagttgg ctcacgttgt ttccttttag ttgtctgtta tgacagaccg gcgcaaaatg
     5761 cttgctgctt ccggtccggt gcaaaaaagc aggtccaaga tactaagatt cggcaaaaag
     5821 cccagcctgt cttcaaaaac ctgcacatag cgcacatcgc ggtacagcgg atcggggcgt
     5881 tgcaggcgcg gtttgaaggc aaaagcatcg cggagatcgc ggtagttttc gggggcatcg
     5941 ggcgcgtagc tttcggtggt ttcaaacgcg accggcaggc gcaaggcttt gagcaggatg
     6001 cggagcaaac cggtattcca ctcaacgagg tactccggtt gctgccggta ccaggcttcg
     6061 agcggctcgg cgtagaagtc gaaaaacggc gatttgcgat aggcgtcgtg tatgctgcgc
     6121 cagtgggtgc gttgccaggg ttcgtggtag gcaattttta cttctcgcaa tggttgcaga
     6181 tacttgcccg acaacaaggg caccgacaac accaaagacc cacccgccgt agcgacggca
     6241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnct ttagtataag gagcgcattg ctccaaaata
     6301 accgaagagg aggctgccca gtgtgcaaaa tattgaatgg taggcagata caatatgtcc
     6361 atcaaggcgc cggcagcagg gtggctcatg tcaggtggtt tcgatgatgc cgttttgcag
     6421 ggcgtagagg gtcagctctg cgggcgactg caattgcagc tttttcataa tgtttttacg
     6481 atgggtataa acggtgtgcg ggctgaggtg cagcatgtct gcaatttgcc gggcaatgta
     6541 gcctttgacg accaactgaa ccacttcgat ctcgcgggcc gacagtggcg agggtgcgca
     6601 agtagcggta gttcggccta agcttttttc caacagcaca tccaatatct tggaacagaa
     6661 aaacttatct cccctgacgg tagcgcgtac ggcatcggat acctcctgtt cgccgcagga
     6721 tttggtgagg aagctgagta cgccatgatc caaaaccctc aaaatggaat ttttgtcgct
     6781 gtcgtcgctg atggccaata tgcgcagggt agggtagagg ccggatacga gggcaatgac
     6841 ctctctgctg aagcgcttgg gcgaattgta gtcaatgatg agcatatctg ggcggagggc
     6901 ctgcaattgc tgaaacaatt cctcctctgt tgcggcttcg cccactattt gcacctcggt
     6961 ttgttctgcc agaatacagc gcaaacccgc gcgcactaaa aactgcgcat cggccagcag
     7021 gatggttttg ggctgcgttg attgcatatt ttactttaag tcaggcaaag gtagaaaaat
     7081 cacggcgttc cgtcatcaga cttcaacgtg cgcgcatctc tacaaacaac tccctgccgg
     7141 ctctgggacg tatgctgtgt gagcttattt ccatgataag tatctcaaaa taaggctcga
     7201 agcagttctg atattcagca gccatgcctc cgaagggcgg cccttcctgc tcaaattcct
     7261 gtgcaaacaa cacgcctgcc aatagcccgc cacccctcaa caagtgggcc gcctgccgcg
     7321 cataagcctg ccgttgagcg cggtcaatgg cgcaaaaaaa ggtctgttcg agcatcagat
     7381 caaactccat gtccaactca aaaaagtcgg ctgccaaaag cctttctctc ggaaactccg
     7441 ggcaatgctc caataaatga ccaaaagccg ccggcgccca atcgcatacc cacacctgtt
     7501 caaaaccggc gcggtacaaa aaaatggcct cataagcgcg ccctgctccc ggtatgagaa
     7561 tgcgtatttg tttgtctttt agccgcttaa tgtattccaa caacggcggc gaggcatacc
     7621 cgatgtccca gggagtgtgc tcgttttcgt agcgttggtt ccaataagtt gcggatagat
     7681 cgtgcatgga tttttgattg aagatttttc gttcaaggtt tcgagtttcg tgttcaaggt
     7741 ttcccctcgc gcttagcacc gcccactgat gaccgcccac cgcctactgg ttccgggttc
     7801 aaggttccgc tcagcagata aactgcgcag cagcttcctc ctctccatta gcaagatgaa
     7861 ctgcgcaccg cgccacaggc gcggcccctc ttcgttggca gataaactgc gcagcagatt
     7921 actacgaagt agatcaactg cacagcagat cacaaatgta cgaagcatat ttttaacact
     7981 atttaaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa accgcttaca
     8041 tttgcggcca atagtagttt ttgtgtttat ttgtttgggt tagaggccct tgctcaagtc
     8101 attgctgcaa gggttttttt tgtaaaacct cattgtattc agaaaaaagc tatatttggc
     8161 ccgtcatcat cccaagaaca agttatgcag aagcatccaa ctgctgcatg gtctgtatta
     8221 aacaggcgca aagcatcttg ctgtctttgc catttccct
//
LOCUS       DAORLF010000201         8203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4735_length_8203_cov_1.579774, whole genome shotgun sequence.
ACCESSION   DAORLF010000201 DAORLF010000000
VERSION     DAORLF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4735_length_8203_cov_1.579774"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    271..360
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<361..1526)
                     /locus_tag="PK198_15310"
     CDS             complement(<361..1526)
                     /locus_tag="PK198_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40158.1"
                     /translation="MKTYHSHLASPCLLLATFLIAGLCSIQAQIEPQNYDYRTFRFPE
                     INRRALELFPSLSMDGTDSRNRRFGIPDQNSSYTTAGLNAQYGYFHNSLAEQIQHTGS
                     LYQLASFSRIERTDPPSRSETNEYEARLSTEGINRFYYGKRRSFIEINWDIDAVFTAS
                     NHINTSLTDVRKERFYAYLFSVPLRFGWGRIEPIDDVFLAHFMADDMLEAGILDHPLP
                     QESLFELGRVMADVRNQRIFDARKKRMYELSQLDNWFKDFGYAKVDNILYYKTLADNW
                     LFGFNNTRFSGSRFSFGITPQADYWAPHQQEPSVINMLAMQAIYEGSTPLNQHWQIGY
                     TVQTELAFLSNENWLNNSVSRPGLSAEFKWGFYPNSRTRFTGYMGVDYQLFLRT"
     gene            complement(1546..2931)
                     /locus_tag="PK198_15315"
     CDS             complement(1546..2931)
                     /locus_tag="PK198_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40159.1"
                     /translation="MHNYPLTACCLRRVASMVFLCFATILSISAQSSPTKYHFKEFKL
                     PEINRRALDIKPNLSGALIRNLLSGKDLLSSSSKITQNIGLQYSYFHNSKRKQIFHNL
                     YIGQSYNAINQLSDFHSSFNPRINRQFGSNLYHSAVNRYYFTSRHFSEINWDVQGSFF
                     DRRTLTSAASLLTQTNTYFYSISVPLKTGIGRVEPVQDVFSAAFMAEDMLQVGILDSP
                     MEQEELFELGRVMADARNLRIFDARKKRMYELSQLDNWFKDIGKAKVDNILYYNTMAD
                     NWLFAFSGVRYSGRRISTSVTPGFQYFGNEGNAPNTRLSFSGAFLLEQFLPLSRTWQL
                     DYSINVGISRNFVSVDVYSEYRFFTPFMAAQLELGCFPNSRTSIHFNARVAGEFYGAY
                     PSILQTFASFHASGNIFLNYRTRLYADVHWDYYRTSDNAIYQHPRAFLPQAGLRNIDY
                     GANIRLIYSLF"
     gene            3018..3329
                     /locus_tag="PK198_15320"
     CDS             3018..3329
                     /locus_tag="PK198_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40160.1"
                     /translation="MALFNFLKTTKPIQFDFKPRFYDEEKEGRQQRLKELEGLSEHDT
                     EGIKNRISGGFRRSTDMSALHLRRKMVLRSNLMVMALVMLLALGAFLLLNVYLPGWLN
                     K"
     gene            3415..>4188
                     /gene="mutL"
                     /locus_tag="PK198_15325"
     CDS             3415..>4188
                     /gene="mutL"
                     /locus_tag="PK198_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRF40161.1"
                     /translation="MADLIQLLPDAIANQIAAGEVVQRPASAVKELMENAVDAGATEI
                     RLIVKDAGKTLVQVTDNGCGMSETDARMCFERHATSKIRQSSDLFAIRTMGFRGEALA
                     SIAAVAQVEMRSRRPEDELGTRILIEGSELKAQEPCQTAPGTSISMRNLFYNVPARRN
                     FLKSNVVEMRHIIDEFQRVAIANPEVFFSLHHNNEELFHLPASNLRQRLVGIFGIAYN
                     KRLVPVKEDTDILNIDGFVGKPEFARKTRGEQMFFINGRF"
     assembly_gap    4189..4367
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4368..>4653
                     /locus_tag="PK198_15330"
     CDS             <4368..>4653
                     /locus_tag="PK198_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40162.1"
                     /translation="VRHALGSHSITPSLDFDQDITLQQAPTAPRQESYTIKTIPSSFS
                     PPSTAHRTPPTADRIPHTADRIPPNDNLRHWEKLYEGLDELAPAQPETLII"
     assembly_gap    4654..4747
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4748..5271
                     /locus_tag="PK198_15335"
     CDS             <4748..5271
                     /locus_tag="PK198_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HRF40163.1"
                     /translation="HIKSGFLLIDQQAAHERILYEQYLRVLGEQAASVQHQLFPRTVA
                     LPPADAVLLREIRPQVNLLGFDIQEFGQNTFVINGLPAEMAGKKDELAILETLLEQFR
                     QNNELQLDVRDNLARSMARSAAIRRGAPLTVPEMQSLIDRLFACEMPYKSPAGRQCFV
                     TFELEDLARRFES"
     assembly_gap    5310..5345
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5346..6105
                     /locus_tag="PK198_15340"
     CDS             <5346..6105
                     /locus_tag="PK198_15340"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF40164.1"
                     /translation="INILMFVVTELIFPSWKGSLAMYYPGSEQFQPIQLVTHFFMHGG
                     WMHLFFNMYALAMFGPPVEMLYRSKRFLFYYIVCALGAAVLHNLYTWYDVSQLQTAME
                     AFQAAPSYDHYWQFFNQINPKHFTLYGNELVDDISRQLLNGDTGQAGSALQLMNDLVE
                     RKMNVPAVGASGAIYGLLLAFGMQFPNHQLRLIFLPVPIKAKYFIPLLMVAEFYFGLQ
                     GFDWDPIAHFAHLGGALAGFIMIMIWRKTGARLY"
     assembly_gap    6184..6196
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6256..7200
                     /locus_tag="PK198_15345"
     CDS             6256..7200
                     /locus_tag="PK198_15345"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF40165.1"
                     /translation="MIQSIWEEIRREFRHGNMVTRLIIANVSVFVLVWLIYLLMLLTL
                     GHSGNHHTYFDKMIEYLCMPADWKHLLLRPWSLFTYMFLHQEPLHIFFNMIMLYWFGR
                     IVGDFIGDHRVLPIYLIGGLAGALLFFVFANAFFDADAIMLGASAAVMALAVAAAALA
                     PDYLMRLILLGDVKLKYVVAVLLILDLVAIGKESNTGGHVAHLGGALIGGLFVQQLRA
                     GNDWSAPINRLLDKILQLFRSIKGQPRKRGPHVVYRNPVKATTEAGGKQRRKPSEDSN
                     GLSYQEQLDAILDKIKQSGYNSLSTEEKEFLFNASNRK"
     gene            7197..>7438
                     /locus_tag="PK198_15350"
     CDS             7197..>7438
                     /locus_tag="PK198_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40166.1"
                     /translation="MKTKLFQLIYRVQLLLALLTLAAFYAPSVSPNDFWPAAFLSLLI
                     PVLLVAHIGFVLWWLLHSRYYLLVSLACLVAGWGHL"
     assembly_gap    7439..7599
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7600..7867
                     /locus_tag="PK198_15355"
     CDS             <7600..7867
                     /locus_tag="PK198_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40167.1"
                     /translation="DVLCLQEFPRSGNRMEQLSARLAESTRLRHHYSDPNGNFALFSA
                     FPISNPQTQYFPNRSNGYQQADLRIGDRNIRVINAHLQTNAHSR"
     gene            7833..>8203
                     /locus_tag="PK198_15360"
     CDS             7833..>8203
                     /locus_tag="PK198_15360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF40168.1"
                     /translation="MRICKPTRIADKIASEGDFKEKQTWLDIRGVMGRFRRSARQRAL
                     QSEEIARLIAGSPHPVLLCGDFNDTPYSYTYHTLANGLRDAFAAKGRGLGITYAGRIP
                     ALRIDYILADPQFRIHDHRIE"
BASE COUNT         1829 a         1923 c         2006 g         1872 t
ORIGIN      
        1 caatcggatc tgtattgcgt ccaatatcag cagcaagtcg ggtctcttca tgagaagggc
       61 aatcagttcg gaggtattca taacgacttg attgtctctg tttttgaaac aacgatgccg
      121 gacgtgattt ggttggcgcc gctcaggttt cagaagaagc tgtacgtcaa ctccaaagcg
      181 ccggagacat tcaacctgac agcgtcggcc cttggaatga gtgggtgccc taccacaaaa
      241 tcggctttgg gtgtacggta atgcctgccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 caggtgcgca aaaataattg ataatcaaca cccatgtagc ctgtgaaacg ggttcgggaa
      421 ttggggtaaa aaccccactt gaattcagcc gacaagccgg gccgggaaac ggagttgttc
      481 agccaatttt cgttcgataa aaacgccagt tccgtttgaa cggtatagcc tatttgccaa
      541 tgttgattca acggtgtgct gccctcatat atggcctgca tggccagcat attgatgacc
      601 gatggctctt gctgatgcgg cgcccaataa tcagcttggg gtgtaattcc gaatgaaaaa
      661 cgacttcctg aaaagcgggt attgttgaaa ccaaacagcc agttatcggc cagtgtcttg
      721 taatacagaa tgttgtccac tttggcatag ccgaaatctt tgaaccaatt gtccaattga
      781 gacaattcgt acatgcgttt ttttcgggcg tcgaaaatgc gttggttgcg gacatcggcc
      841 atcacgcggc ccagttcgaa aaggctttcc tgcggaagcg gatgatccaa tatgcctgct
      901 tcaagcatgt cgtcggccat gaaatgtgca agaaacacat cgtctatggg ttcaatgcgg
      961 ccccagccga accggagcgg cacagagaac aaataggcat agaaccgctc ttttcgaaca
     1021 tccgtcagtg aagtattgat gtgattgctt gctgtgaaaa cggcatctat atcccagttg
     1081 atttcaataa aggagcgccg cttgccataa taaaaacgat tgatgccctc cgtgctcaat
     1141 ctggcctcgt attcgttcgt ttccgaccgg cttggcggat ccgtacgctc tatccttgaa
     1201 aaagaagcca actgatacaa gctgccggtg tgttgtattt gttcggcaag actgttgtgg
     1261 aaatagccgt attgagcgtt aagtcctgct gtggtgtaag aagagttttg gtctggaatt
     1321 ccaaaacgac gattgcggga atcagtgccg tccatactca gcgacggaaa caactccagc
     1381 gcccgccggt tgatttcggg aaacctgaag gtccggtagt cgtagttttg aggctctatc
     1441 tgggcctgga tggagcaaag gccggcgatc aaaaaggtag ccaataaaag gcatggtgaa
     1501 gcgagatgac tgtgataagt tttcatgtcg aatgtaattt aggattcaga acaagctgta
     1561 tatgagccga atattggctc cgtaatcaat gtttctcaag ccggcctgag gcaaaaacgc
     1621 tctggggtgt tggtagatgg cattgtcgga agtgcgataa tagtcccaat gtacgtcggc
     1681 gtagaggcgg gtacggtaat tgaggaatat attaccactt gcatggaagg aggcgaaagt
     1741 ctgcaaaatt gagggatatg cgccgtaaaa ttcgccggct acccgagcat tgaaatgaat
     1801 actggttctc gaattgggga agcagcccag ttctaattgg gctgccataa atggtgtaaa
     1861 aaagcggtat tccgaataga cgtcaacact gacaaagttg cgcgagatac ctacattgat
     1921 ggagtaatcc aactgccagg tacggctcag tggtagaaat tgttccaaca aaaaagcacc
     1981 cgaaaaactc agccttgtat tgggcgcatt gccttcgttt ccgaaatact gaaagccggg
     2041 cgtcacgctg gtagaaatac gcctgcctga atatctgacg ccggagaatg caaacagcca
     2101 gttgtcggcc atggtgttgt aatacagaat gttatctact ttggccttgc cgatgtcttt
     2161 gaaccagtta tccaattgag acagctcata catgcgtttt tttcgggcgt caaaaatgcg
     2221 taaattgcgg gcatcggcca tcactcggcc cagctcgaag agttcttctt gctccatagg
     2281 gctgtctaaa atgccgacct gcaacatatc ctctgccata aatgccgcag aaaatacgtc
     2341 ctggacaggt tccacccgtc cgatgccggt tttgagaggg acggatatgg agtagaaata
     2401 agtgttggtc tgagtaagaa gcgaggcagc gcttgtgaga gtgcgccggt caaaaaagga
     2461 gccttgaaca tcccaattga tttcggagaa atgcctggag gtaaaataat agcgattgac
     2521 ggctgaatga taaagattgc tgccgaactg tcggtttatt ctggggttga acgaagaatg
     2581 gaaatcgctg agctgattga tggcgttgta ggattgtcca atgtacaggt tgtgaaaaat
     2641 ctgtttgcgc ttgctgttgt gaaaataact gtattgtaaa ccgatgtttt gcgtgatttt
     2701 gctggagctg gacaataggt ccttgcctga gaggaggttt cgtatgagcg cgccactgag
     2761 attgggcttg atgtctaacg ctcttctatt gatttccggc agtttaaatt ccttgaaatg
     2821 atacttcgtt ggcgagcttt gcgccgagat gctgaggatg gtcgcaaagc aaaggaaaac
     2881 catggatgca acgcgacgca ggcagcaggc agttaaaggg tagttgtgca tatcttgtct
     2941 tataagaggt tttgagccga caagatagcc aacatctctt ctaaaatgcc taactttgct
     3001 tatcaaaatc gaatatcatg gcacttttta attttttaaa aaccaccaag ccgattcagt
     3061 tcgacttcaa accgcgtttc tacgatgaag aaaaagaagg ccggcagcag cgcttgaaag
     3121 agttggaagg gctgagcgaa cacgataccg agggcataaa aaaccgcatc tccgggggct
     3181 ttcgccgcag caccgatatg tcggccctgc acctgcgtcg caaaatggtg ttgcgctcca
     3241 atctcatggt catggctttg gtaatgctgc tggcattggg cgcatttttg ttgctcaatg
     3301 tttacctgcc cggatggctt aataaatgag gctttgaggc tgggaacccg cctacgccga
     3361 ggctttggcg ggtgaaagga gtattattga ataaccaaaa agtaagatga aaacatggct
     3421 gatctcattc agcttttacc ggatgccatt gccaatcaga tagccgcagg agaagtagta
     3481 cagcgccctg cctcggctgt caaagaactg atggaaaatg cggtggatgc aggcgctacc
     3541 gaaatccggc tcatcgtaaa agatgccggc aaaacccttg tgcaagtaac cgacaacggc
     3601 tgcggcatgt cggaaaccga tgcgcggatg tgctttgaac ggcacgcaac ttccaaaata
     3661 cgacaatcga gcgacctctt cgccattcgc accatgggct ttcgcggtga ggctttggcc
     3721 tccatcgccg ccgtggcaca ggtggaaatg cgcagccgcc gcccggagga cgaacttggc
     3781 acccgcatcc tgatagaagg ctcggaactg aaagcacagg aaccctgcca gacggcgccc
     3841 ggcaccagta tctccatgcg caacctgttt tacaacgtac ccgcccggcg caacttcctc
     3901 aaatccaacg tggtagaaat gcgccacatc attgacgaat ttcagcgcgt ggccatcgcc
     3961 aacccggagg tgtttttttc gctgcatcac aataatgagg agttgtttca cctgccggct
     4021 tccaatctgc gccagcgctt ggtaggcatt ttcggcattg cttacaacaa gcgtctggtg
     4081 cctgtaaaag aagatacgga tattctcaac attgacggct ttgtgggcaa accggaattt
     4141 gcacgcaaaa cccgcggcga acagatgttt tttatcaacg ggcgcttcnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta cgccatgccc
     4381 tcggcagcca cagcatcacg cccagcctcg atttcgatca ggacatcacc ttgcaacaag
     4441 ccccgacggc cccgcgccag gaatcttata ccatcaaaac catccccagc agcttctcac
     4501 caccttccac cgcacaccgc acaccgccga ccgccgaccg cataccgcat accgccgacc
     4561 gcataccgcc caacgacaac ctccgccatt gggaaaaact gtatgaaggc ttggacgaac
     4621 ttgcacctgc ccaaccagaa actctcatca tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnngcc atatcaagtc gggttttctg ctcatagacc agcaggccgc ccacgagcgc
     4801 atcctctacg agcaatacct gcgtgtattg ggcgaacagg ccgcctccgt tcagcaccag
     4861 ttgtttccgc gcaccgtggc cctgccgccc gccgacgccg tgctcctgcg cgagatacgg
     4921 cctcaggtca atttattggg attcgatata caggaatttg gtcagaatac attcgttatc
     4981 aatggattgc ctgctgaaat ggccggcaaa aaagacgagt tggccattct ggaaaccctg
     5041 ctggaacagt tccggcaaaa caacgaactg caattggacg tgcgcgacaa cttagcccgc
     5101 tccatggccc gcagtgccgc cattcgtcgg ggagcgccgc tgaccgttcc ggaaatgcaa
     5161 tcgctcattg atcggctgtt tgcctgcgaa atgccgtata aaagccccgc cggtcggcag
     5221 tgttttgtta cttttgaact ggaagacctg gctcgacgtt ttgaaagctg atttttgaaa
     5281 agcgcccgac aatgaatcaa atcacccatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnncatca acatcctcat gtttgtggtc acagagctga tttttccttc atggaaaggc
     5401 tcgttggcca tgtactaccc ggggtcggag cagtttcaac ccatacagct tgttacgcac
     5461 tttttcatgc acggcgggtg gatgcacctg tttttcaata tgtatgcgct ggccatgttc
     5521 gggccgccgg ttgagatgtt gtaccgctcc aagcgctttt tgttttatta cattgtctgc
     5581 gcgttggggg cggctgtttt gcacaattta tacacctggt acgatgtctc gcaattgcaa
     5641 actgcgatgg aagcgtttca ggccgcgccg agttacgacc actactggca gtttttcaat
     5701 cagatcaatc cgaagcattt tactttgtac ggcaatgaat tggtggacga tatcagccgt
     5761 cagttgctga acggcgatac cgggcaggca ggttcggcgc tgcaattgat gaatgacctg
     5821 gtagagcgca agatgaacgt tccggccgta ggcgcatcag gggccatata cgggctgttg
     5881 ctggctttcg ggatgcagtt tcccaatcac cagttgaggc tcattttttt gccggtaccc
     5941 atcaaggcca agtattttat accgctgctt atggtcgccg agttttactt cgggcttcag
     6001 ggctttgatt gggacccgat tgctcacttt gcgcatttgg gcggcgcgct ggcgggtttt
     6061 attatgatta tgatttggag gaagactggg gcgaggttgt actagtatgc agtaggcagt
     6121 tggcggtagg cggtatgcgg tatgcggtag gcggtaggca gttcgtagtg tgcagctaca
     6181 ttannnnnnn nnnnnncgca tactgcacac tgcaaaccgc ccacttagat taaaagacac
     6241 aaacaagaaa ggacaatgat ccagtctatt tgggaagaaa taaggagaga gttccggcac
     6301 ggcaacatgg tgacgaggct catcattgcc aatgtatcgg tctttgtatt ggtgtggctg
     6361 atctacctgc tcatgctgct gacgctgggc catagtggca accatcacac ctacttcgac
     6421 aaaatgatcg agtacctgtg tatgcctgcc gattggaaac atttgttgct gcgcccctgg
     6481 agcctgttta cctatatgtt tctgcaccag gagcctctgc acatcttttt caatatgatt
     6541 atgctgtatt ggtttggccg catcgtggga gatttcatcg gcgatcaccg ggtgctgccc
     6601 atttacctca tcggaggatt ggcgggtgca ctgttgtttt ttgtgtttgc caatgccttt
     6661 tttgatgccg atgccatcat gttgggtgct tcggcggccg ttatggcctt ggcggtggct
     6721 gccgctgctc tggcccccga ctacctgatg cggttgattc tgctgggcga tgtgaagttg
     6781 aaatatgtgg tagccgtgct gctcatcctc gatttggtgg ccataggtaa agaatccaac
     6841 accggcggcc acgttgccca cctcggcggc gcactgatag gcgggctttt tgtgcaacag
     6901 ttgcgggcag gaaacgactg gtcggcgcct attaaccggc tgttggacaa gattttgcaa
     6961 ttgttcagaa gcatcaaagg ccagccccgc aaacgcggcc ctcatgtagt gtatcgcaat
     7021 ccggtgaaag ctaccaccga agctggcgga aaacaacgcc gtaagccctc cgaagacagc
     7081 aacggcctca gctatcagga gcagttggac gccattttgg acaagatcaa gcaatcgggc
     7141 tacaacagcc tcagcacaga ggaaaaagaa ttcctgttca acgccagcaa ccgaaagtga
     7201 aaaccaagct gttccaactc atttaccgcg tgcagttgtt gctcgctttg cttacgctgg
     7261 cggcgtttta tgcgccctcg gtcagtccca acgacttctg gccggcggct tttcttagtt
     7321 tgttgatccc cgttttgctc gttgctcata ttggttttgt gctctggtgg ttgttgcata
     7381 gccggtatta tttgctcgta tctctggctt gcctcgtggc gggctggggg catctgcann
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gacgtgctgt gtttgcagga
     7621 gtttccccgc agcggcaacc gcatggaaca actcagcgcc cgcctcgccg aaagcactcg
     7681 tctgcgacac cattacagcg atcccaacgg caattttgcg ctgttttcgg cctttcccat
     7741 ctccaacccg caaacgcagt attttccgaa tcgttccaac ggctatcagc aggccgatct
     7801 ccgcatcggc gaccgcaata tcagggtcat caatgcgcat ctgcaaacca acgcgcatag
     7861 cagataaaat tgcctccgaa ggcgatttca aggaaaaaca aacctggctc gatattcgcg
     7921 gcgtcatggg gcgcttccgc cgctcggccc gtcaacgcgc cctgcaatcg gaggaaattg
     7981 cccgcctcat agccggcagc ccgcatccgg ttttgctctg cggagacttc aacgacacgc
     8041 cttactctta tacctatcat acgctggcca atggcttgcg ggatgctttt gctgccaaag
     8101 gccggggctt gggcatcact tatgccgggc gcatccccgc gctgcgcatt gactacattc
     8161 tggccgaccc gcagtttcgc attcacgatc atcgcatcga aaa
//
LOCUS       DAORLF010000202         8172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4752_length_8172_cov_1.973143, whole genome shotgun sequence.
ACCESSION   DAORLF010000202 DAORLF010000000
VERSION     DAORLF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_8172_cov_1.973143"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>927
                     /locus_tag="PK198_15365"
     CDS             <1..>927
                     /locus_tag="PK198_15365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF40169.1"
                     /translation="VTVVFDERLVPHVFAENETDAVFAQGYVTARYRLWQMDFATRAA
                     AGRLSEVVGEKALDRDRGQRRKGMLMAARNAVAAWEKSPEEMALVEAYTNGVNAYIQS
                     LAPADYPLEFKLLDYAPEPWTPLHSALMMKNMAETLCFRSDDLAAYSSLQMWGREMFD
                     FLHPSYNPKQSPIIPAGTPWNFKPIEARPDSIPSPIMMGDLLPYPLLPQSPSGIGSNN
                     WAVSGKKTASGHPILSNDPHLTLSLPSIWFEMQIHAPEYQAYGVTIPGLPGIVIGFND
                     QQAWGVTNVGHDVVDWYRIAWMDDEKTRYLLD"
     assembly_gap    928..1038
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1039..>1302
                     /locus_tag="PK198_15370"
     CDS             <1039..>1302
                     /locus_tag="PK198_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF40170.1"
                     /translation="EDPDSPYRDLAMHWIAHEVQEKMDFYELGAFYRLGQAKTHQQYT
                     EALRNFENPAQNFVMANNTGDIAIRVNGKFPIKTRDQGRFVQD"
     assembly_gap    1303..1482
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1483..2263
                     /locus_tag="PK198_15375"
     CDS             <1483..2263
                     /locus_tag="PK198_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF40171.1"
                     /translation="TLEEKNGMTVKDMMALQNDNRSILAEEALPLLLKLLDRNGLSPE
                     ERTWVGWLEKWDLHFNAGEKAPMVFSEWLRQTHRRAFDEIYVLEDSIPLLKPENWRLL
                     ELLEQAPDHIIFDQKGTPAKETAAQIVTAALHDVAEEWAAKFSESDYNWATHKSTDIM
                     HLARIPAFSKMDLNVGGYGDAPNAIKGTTGPSWRVVVELGPEVKAWGIYPGGQSGHPG
                     SRYYDNRILPWMKGEYDELYIMKRPDERSKPELFGVVFSKK"
     gene            2392..2736
                     /locus_tag="PK198_15380"
     CDS             2392..2736
                     /locus_tag="PK198_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40172.1"
                     /translation="MKSILLFIGILLLGYAAHIFMPWWAVYGVALLLSALVGLRPGRS
                     FFIGFLAAFALWAGTAYWLHQSGEGILTEKIGEMLGGLSVPAVVSITALLGGLLGGLG
                     AWTGSSGRRLLR"
     gene            2784..3179
                     /locus_tag="PK198_15385"
     CDS             2784..3179
                     /locus_tag="PK198_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRF40173.1"
                     /translation="MASKEDKRYEIARKRVEEKKKFYQHLTTYLTMSVFFVFINLFTS
                     PGRWWFIFPVLGWGVGIVSQYFKVFGPPGFNTDGDWEERALQEELRKMGVPESELQQT
                     TPEPNVAEAGDEPLELKELQKKYDERDLV"
     gene            3245..3610
                     /locus_tag="PK198_15390"
     CDS             3245..3610
                     /locus_tag="PK198_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40174.1"
                     /translation="MKNFLLPVAMMLFSFGLAAQNDSIVRPLEGDLVMVRLIERTPTS
                     FWQSRMIISDGSGEPQSILLQRIGNNSIMNDNVRLISKIFNEITRQGYALSAPPASNM
                     SEGSTLYFDYFFTKDKKEK"
     gene            complement(3640..4143)
                     /locus_tag="PK198_15395"
     CDS             complement(3640..4143)
                     /locus_tag="PK198_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40175.1"
                     /translation="MKQLTILLFSALAAMILASCVKRPDYPIEPVLNFIGMNKQIIAQ
                     GSPSAAADTLAITFGFTDGDGDLGHPDGTLDIFLTDSRDGFEETLKLPVIPEQGSGNG
                     ISGEITVLVFNKPFNICCTYPNGQVPCTPSTVFPTDTFSYSIRIRDRAGHFSNSVQSE
                     RITILCN"
     gene            4150..>4595
                     /locus_tag="PK198_15400"
     CDS             4150..>4595
                     /locus_tag="PK198_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRF40176.1"
                     /translation="MNSTFEHIRSTLRERIVQVQPDDFNALALDVFRFQAEHNSLYNR
                     YLELIGKKIQAVETVQEIPFLPIELFKTYDIQTGNWTPERIFSSSGTTGQATSGHLLH
                     DPDWYRMGARRAFARQYGPVENFCFLALLPSYLERNNSSLVFMAED"
     assembly_gap    4596..4613
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4614..5146
                     /locus_tag="PK198_15405"
     CDS             <4614..5146
                     /locus_tag="PK198_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRF40177.1"
                     /translation="GFYLYNMADLVKVLSDCVQNKIPTVLLGVSFALLDLAEAFPMPL
                     EQVIVMETGGMKGRRAELTREQLHQVLQAAFQVQHIHSEYGMTELLSQAYSPGNGRFY
                     PAPTMRVLAREAADPFAIRTWGQNGALNIIDLANVDTLSFIATDDLGKVAEDHSFEVT
                     GRLDSSEIRGCNLMVE"
     gene            complement(5159..5380)
                     /locus_tag="PK198_15410"
     CDS             complement(5159..5380)
                     /locus_tag="PK198_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40178.1"
                     /translation="MLKNKTFWTIVGFFLAGTGLIALMLSLVGVQLAGLAWLSKISGL
                     LAFLVHLLMVLGGFVLIYLMRSDFRGDDL"
     gene            5459..6307
                     /locus_tag="PK198_15415"
     CDS             5459..6307
                     /locus_tag="PK198_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRF40179.1"
                     /translation="MIKIIECPRDAMQGIKEFIPTPTKTAYLNQLLQVGFDTLDFGSF
                     VSPKAIPQMRDTAEVLAGLDLSHTRTRLLAIVANRRGAEDAVAFPEISYLGYPFSISE
                     TFQQRNTNASIEESLERVEEIQNLCISSGKQLVLYISMGFGNPYGDPWNVDIVQRWVA
                     RLTGMGIGIFQLSDTIGVAGPDSIRYLFSHLIPAYPQVEIGAHLHTHPATWREKTAAA
                     YESGCRRFDGAIMGFGGCPMAKDDLTGNMPTEKMMAYLAEQESLGLNMKALAEAMRMA
                     PSVFPL"
     gene            complement(6302..7099)
                     /locus_tag="PK198_15420"
     CDS             complement(6302..7099)
                     /locus_tag="PK198_15420"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF40180.1"
                     /translation="MIHYQEFRKFALCKYFIKITYTTDKSILTKSRQEKWHLIYPESD
                     GLPMTENTLQFEWIVLIKLGLEACFAQRNDVFIAGDLFWYPVEGQPGVRLAPDVLVVL
                     GRPKGHRGSYMQWVEDGIPPQVVFEILSPGNRSREMHNKWEFYRQHGVQEYYVYDPDR
                     NNFIVYIRQQDDLTALPADQIRDFISPLLGVRMVWGEEKLELYHPDGKKFLSYMELLE
                     QDKVAQQFAEEQAKRAENAEQRAEMEAKRAEKLADMLRKLGIDPNGL"
     gene            7368..>7732
                     /locus_tag="PK198_15425"
     CDS             7368..>7732
                     /locus_tag="PK198_15425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRF40181.1"
                     /translation="MLQRGITGFFFILVMVGGIYGGRYTFVGLFAVITAGCLWEYLSL
                     VLDRGRKRDLIRKMLGVLLGLTPFAGNSLMHLGLVADEALFVSGSLLVFFPLLFMAFI
                     YEMYAQSEHPFTNVGYAVLG"
     assembly_gap    7733..7801
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7802..>8172
                     /locus_tag="PK198_15430"
     CDS             <7802..>8172
                     /locus_tag="PK198_15430"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRF40182.1"
                     /translation="AGAVMGLLILTWGNDTAAYVFGSRWGRRPLSPRLSPKKTWEGAL
                     GGGVFTLAVGWILSLFITSLSPLNWIMLALIVAVFGTFGDLVESMLKRSFEVKDSGRL
                     LPGHGGLLDRLLSLKGAGCDT"
BASE COUNT         1873 a         1949 c         2096 g         1876 t
ORIGIN      
        1 cggtgacggt ggtattcgac gagcgcctcg taccgcacgt ttttgctgaa aatgagacag
       61 atgccgtttt tgcgcaaggt tatgtgaccg cacgttaccg cctttggcaa atggacttcg
      121 ctacccgtgc cgccgccggt cgcctgtcgg aagtggtggg tgaaaaagct ttggatcgag
      181 accgtggaca acggcgcaaa ggcatgctca tggctgcccg gaatgcagta gccgcctggg
      241 aaaaatcgcc ggaggaaatg gctttagtgg aggcttacac caacggcgtc aatgcttaca
      301 tacaatcgct cgcgcctgct gattatccat tggagttcaa actcttggat tatgcgcccg
      361 aaccctggac gccgctgcac tcggccctga tgatgaaaaa catggcagaa acgctgtgtt
      421 tccggtcgga cgatctggcg gcctacagct cccttcaaat gtggggccgc gagatgttcg
      481 attttctcca tccatcctac aatcccaaac aatcgccgat catacctgcg ggcacgccct
      541 ggaatttcaa accgatagaa gcccgccccg acagcattcc gtctcccatt atgatgggcg
      601 atctgttgcc ctatccgctc ttgccgcagt cgccgtcggg catcggcagc aacaactggg
      661 ccgtgtccgg caagaaaaca gcatcgggcc accccatact cagcaatgac ccgcacctca
      721 cgctcagcct gccgtcaata tggtttgaaa tgcagataca cgcccctgaa tatcaggctt
      781 atggagttac cattccgggc ctgccgggca tcgtcatcgg cttcaacgac caacaggcat
      841 ggggtgttac caatgtgggc catgacgtag tggactggta ccgcattgcc tggatggacg
      901 acgaaaaaac gcgctatctg ttggacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnat gaagaccccg actctccgta ccgcgatctg gcgatgcact
     1081 ggattgccca tgaagtgcag gagaaaatgg acttttacga attgggcgca ttttaccgct
     1141 tggggcaggc caaaacacat cagcaataca cagaggcgct gcgcaatttt gaaaacccgg
     1201 cccaaaattt tgtcatggcc aataacaccg gagacatcgc cattcgggta aacggcaaat
     1261 ttccgatcaa aacacgcgat caagggcgat ttgtacaaga cgnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaccttgg aggagaaaaa
     1501 cggcatgacg gtcaaagaca tgatggccct gcaaaacgac aaccgcagca tcctggccga
     1561 ggaggctttg ccgttgttgc tcaagctgtt ggatagaaac ggcctcagcc cggaagaacg
     1621 aacctgggta ggctggctcg aaaaatggga cctccatttt aatgccgggg agaaagcgcc
     1681 gatggttttt tccgaatggc tgcggcaaac acaccgccgc gcctttgatg aaatatacgt
     1741 tttggaggac tccatcccgc tgttgaaacc cgaaaactgg aggctgttgg aactactgga
     1801 acaagcgccc gatcacatca tttttgatca aaaaggtacg cccgccaaag aaacggccgc
     1861 tcaaatcgtg accgccgcgc tgcatgatgt agcagaagaa tgggctgcga aattcagcga
     1921 aagcgactac aactgggcaa cgcacaaaag cacggacatc atgcacctgg cccgcattcc
     1981 ggccttttcc aaaatggacc tgaacgtggg cggctacggc gatgcgccca atgccatcaa
     2041 aggaaccacc ggcccctcat ggcgggtagt ggtggaattg gggccggagg tgaaggcttg
     2101 gggtatctat cccggcgggc aaagcgggca tcctggcagt cggtactacg acaaccgcat
     2161 cctgccctgg atgaaaggcg aatacgacga attgtacatt atgaagcgcc cggatgaacg
     2221 ctctaagccg gaactgttcg gagtggtttt ttctaaaaag tagtccggct cgcggctttt
     2281 gggtaactta aacgacatac attgtaacta cttagcatct ttttcagggc cacaagctag
     2341 tggcttgtag ctaacagcct atgggcgatg ctaaataaat accatagatt catgaaatca
     2401 atccttctct tcatcggcat ccttttgctt ggttatgcgg cgcatatttt catgccctgg
     2461 tgggcggttt acggcgttgc gctgttgctg tcggcccttg tcgggttgcg gcccggtcgc
     2521 agttttttca tcggattttt ggcggccttt gccctgtggg cgggtacggc ttattggctg
     2581 catcaatcgg gcgaaggcat tctgaccgaa aaaatcggcg agatgctggg cggtctttcg
     2641 gtgccggcag tggtgagtat tacggcctta ttgggcggtt tgctgggcgg actcggcgcc
     2701 tggacgggca gttcggggcg gcgactgctg aggtaaaacc cgatgcgcgc aaggcttgtt
     2761 ttatacccaa attcatttat aacatggcgt caaaagaaga caaacgttac gaaatagcca
     2821 gaaagagggt ggaggaaaag aagaagtttt accaacatct gaccacttac ctcacgatga
     2881 gcgtcttttt tgtgttcatc aatttgttta cctcgccggg gcgctggtgg ttcatttttc
     2941 cggtgttggg ctggggcgtg ggcattgttt ctcagtactt taaggttttt ggaccacctg
     3001 ggtttaacac cgacggcgac tgggaagaac gggcgctgca agaggaattg cggaaaatgg
     3061 gcgtgccgga atcggaattg caacaaacaa cccccgaacc caatgtggca gaagcaggag
     3121 atgagccatt ggaactcaaa gaattgcaaa aaaaatatga cgagcgggat ttagtatgag
     3181 aaccttgtat gctcacaaat gccgtcaacg acatttctca atctaacttc taagtgctat
     3241 gaaaatgaaa aacttcctcc tgccggtggc catgatgctg ttcagctttg gcctggccgc
     3301 tcagaatgac tccattgtgc gcccactcga aggagacctt gtgatggtca ggctgataga
     3361 gcggacgcct acttccttct ggcaatccag aatgattatt tctgatggtt cgggagagcc
     3421 tcagagtatc ttattacaac gaattggaaa taacagcatc atgaacgata acgtgaggct
     3481 gatctccaag attttcaatg aaataacccg acaaggctac gctctgtctg cgcccccggc
     3541 cagcaacatg tctgaaggtt ctactttgta ttttgattat ttttttacca aggacaaaaa
     3601 ggaaaaatga gaaacgccgg gcggcgatct gttttgtgtt caattgcaca aaatcgtaat
     3661 tcgctcactt tgcaccgaat tgctgaaatg ccccgcccgg tcgcgaatgc gaatggagta
     3721 cgagaaagtg tctgtgggga aaaccgtgct gggtgtacac ggcacttgtc cgttgggata
     3781 agtgcagcaa atattgaaag gcttgttgaa gaccagcacc gtaatctcgc cggaaatccc
     3841 attgccggaa ccctgctccg ggatcacggg caatttgagg gtttcctcaa aaccgtcgcg
     3901 gctgtcggtc aggaaaatat caagggtgcc gtcgggatgc cccaaatcgc cgtcgccgtc
     3961 ggtaaatcca aaagtaatgg ccaaggtgtc ggcggctgcg ctgggtgaac cttgagcaat
     4021 gatctgcttg ttcatgccga tgaaattcag cacaggctca atggggtagt cgggccgctt
     4081 cacgcaagag gcgaggatca tggcggcaag agcggagaat aaaagaatgg ttaactgttt
     4141 catggacaat tgaattctac atttgaacat atcagatcaa cgctccggga gcgaatcgtg
     4201 caggtgcagc cggatgactt taacgcattg gccttggatg tctttcgttt tcaggcagag
     4261 cataattcgt tgtacaatag gtatttggag ttgattggca aaaaaataca ggcggtggaa
     4321 accgtgcagg aaatcccctt tttgcccatc gaactgttca aaacctacga catccaaacc
     4381 ggaaactgga cgccggaacg catattcagc agcagcggca ctaccggcca agctaccagc
     4441 ggccatctcc tgcacgatcc cgattggtac cggatgggtg ctcgccgagc ctttgcccga
     4501 cagtatggcc ctgtggaaaa tttctgcttt ttggccctgc tgccctctta tctggaacgc
     4561 aacaattcat ctttggtttt tatggccgag gatttnnnnn nnnnnnnnnn nnngcggctt
     4621 ttatctgtac aacatggccg acctcgtaaa ggtactgagc gactgcgtac aaaacaagat
     4681 tccgacggtt ttattgggag taagttttgc gttgctcgat ctggcagaag cctttcccat
     4741 gcccctagag caggtcattg tgatggaaac aggaggtatg aaagggcggc gcgcagagtt
     4801 gacccgtgag caattgcatc aggttttgca ggctgcattt caagtgcaac acattcattc
     4861 tgaatacggt atgacggaat tgttgtcgca ggcctactct cccggaaacg ggcggtttta
     4921 ccccgcgccc accatgcgcg tattggcccg cgaggccgcc gatccgtttg ccattcggac
     4981 ttgggggcaa aacggcgccc tgaatatcat tgacttagcc aatgtggaca ccctgagctt
     5041 catagccacc gacgacctcg gcaaggtagc ggaagaccac agttttgaag tgacaggccg
     5101 attggacagc agcgagatca gaggctgcaa cctgatggta gagtagggga ggggacgctc
     5161 acaaatcgtc gccgcggaag tcgctgcgca tgaggtaaat cagcacaaat ccgcccaaaa
     5221 ccatgagcag gtgtacaaga aaagccagca ggccgctgat tttactcagc catgccaagc
     5281 ctgccaactg aacgcctacc aagctgagca tcagggcaat aagaccggtt ccggccaaaa
     5341 aaaatccgac gatggtccag aaagttttat ttttgagcat attccgttcg tttgcacaca
     5401 gacaaaagta cgcggaaaat gtttactcag aactgtttct cgaaagctat tagtccttat
     5461 gattaaaata atcgagtgtc caagagatgc gatgcagggc atcaaggaat ttatacccac
     5521 gccgacaaag acggcctacc tgaatcaatt gcttcaggtg ggatttgata cgcttgattt
     5581 cggcagtttt gtatctccca aggccattcc tcaaatgcgc gatactgcgg aagtgctggc
     5641 cgggcttgat ctgagccata cccgtacccg cttgctggcc attgtggcca accggcgcgg
     5701 cgccgaagat gcggtcgcgt tcccggagat ttcttatctg ggctaccctt tttccatttc
     5761 ggaaacattc cagcagcgca acaccaacgc gagcatagag gaatcattgg agcgtgtgga
     5821 agagattcag aacctgtgta tcagctcagg aaaacaattg gtgctgtaca tttcgatggg
     5881 attcggcaat ccgtacggcg acccctggaa tgtggacatt gtgcagcgtt gggtagcgcg
     5941 attgacaggc atgggcatcg gcatatttca actctcggac accattggcg tagccggtcc
     6001 tgacagcatc cgataccttt tttctcatct cattccggcg tatccacagg tagaaatagg
     6061 ggcgcatttg cacacacatc ctgccacctg gcgcgaaaaa acggctgctg cctatgagag
     6121 tggttgcagg cgcttcgacg gcgccatcat ggggtttggc ggctgcccga tggccaaaga
     6181 cgacctcacc ggcaatatgc ctaccgaaaa aatgatggcc tatctggcag aacaggaatc
     6241 actgggcttg aatatgaagg cgctggccga agcgatgcgc atggcgccaa gtgtatttcc
     6301 gctataaacc attggggtct atgcccaatt ttctgagcat gtcggccaat ttttcggcgc
     6361 gtttagcctc catttcagct cgttgctctg cattctctgc gcgtttggcc tgctcctcgg
     6421 caaactgttg cgccaccttg tcctgttcga gcaattccat gtaagagaga aacttcttgc
     6481 cgtcgggatg atacaactcc aatttctcct cgccccaaac catgcgcaca ccgagcagcg
     6541 gactgatgaa atcgcggatt tgatcggcgg gtaaagctgt taaatcatcc tgttggcgga
     6601 tatacacgat gaagttgttg cggtcaggat catagacata gtactcctga acgccgtgtt
     6661 gcctatagaa ctcccatttg ttgtgcatct cgcggctgcg atttccgggc gacagtattt
     6721 caaaaaccac ctgaggggga atgccgtcct ccacccattg catatacgag ccgcgatgtc
     6781 ctttggggcg ccccaaaacc accagcacat cgggcgctaa gcgaacgccg ggttggcctt
     6841 ccaccggata ccaaaagaga tcgccggcaa tgaatacgtc gttgcgctgc gcaaaacatg
     6901 cttcgaggcc cagttttatg agcacaatcc attcaaactg gagggtattt tcagtcatag
     6961 gtagtccgtc tgattcgggg taaatgaggt gccacttctc ctggcgggat ttcgttaaaa
     7021 tagacttgtc tgtggtgtaa gtgattttta tgaagtattt acacagtgca aacttacgaa
     7081 actcctgata atgaatcaag gcgctgctca tttttcccag ggcaaacgca tcaaaaccaa
     7141 gaatgacctc ggagaggtca aatgtttgta gaaaaggtca tttataccaa ttgggacgac
     7201 ctcggagagg tcgcacatga tataataaga caatgtgcga cctctccgag gtcgtctaaa
     7261 cgtgaaaaaa cgcttatttc tacaaagata cgacctctcc gaggtcgtca tatcatttga
     7321 tgcgtttgcc ctgcattttt cctacctttg catttcaagt caggcttatg ttgcaaagag
     7381 gtatcaccgg attctttttt attttagtta tggtaggcgg catttatggt ggtcgttaca
     7441 ccttcgtagg tctgtttgcc gtcatcacgg cgggttgttt gtgggaatac ctgagtttgg
     7501 tactggatcg cgggcgcaag cgagacctca tccgaaaaat gctgggggtg ctgttaggac
     7561 taactccatt tgccggcaat tcgctgatgc acctcggatt ggtagctgac gaagcgctgt
     7621 ttgtatccgg ctctcttctc gttttttttc ctttgctttt tatggctttt atttatgaaa
     7681 tgtatgcgca atccgagcat ccgtttacca atgtgggtta tgcagtgttg ggnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 ngccggcgcc gtcatgggtt tgctcattct cacctggggc aacgatacgg cggcctacgt
     7861 tttcggctcg cgttggggcc gcaggccgct cagtcccagg ctgtcgccca aaaaaacatg
     7921 ggaaggcgcg ctgggtggag gcgtatttac tttggctgtc ggctggattt tgagtctgtt
     7981 tattacctcg ctgtcgcctc tcaattggat catgctggcg ctcatcgttg ctgtttttgg
     8041 tacattcggc gatttggtag aatctatgct gaagcgcagt ttcgaggtta aagactccgg
     8101 gcggcttttg cccggccatg gcggtctttt ggatcgcttg cttagcttga agggtgccgg
     8161 ctgcgataca ag
//
LOCUS       DAORLF010000203         8138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4772_length_8138_cov_1.486082, whole genome shotgun sequence.
ACCESSION   DAORLF010000203 DAORLF010000000
VERSION     DAORLF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4772_length_8138_cov_1.486082"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>395
                     /locus_tag="PK198_15435"
     CDS             <1..>395
                     /locus_tag="PK198_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF40183.1"
                     /translation="RLYPLTLAVSKQLMPVYDKPMIYYPLSTLMSAGIRDILFISTPH
                     DLPHFEKLLGDGSEIGCHFSYVPQYVPNGLAQAFVLGADFIGSDSAALILGDNIFYGN
                     GLSEQLRNSVNPDGGVIFAYQVADPERYG"
     assembly_gap    396..566
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <567..>834
                     /locus_tag="PK198_15440"
     CDS             <567..>834
                     /locus_tag="PK198_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRF40184.1"
                     /translation="DVNKHYLEMGRLHVEVLGRGIAWLDTGTHKSLMQAGQFIEVIED
                     RQGLKIGCIEEVAYDMGFIDAAELERLGHKYLKSGYGEYLLGLL"
     assembly_gap    835..854
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(935..1951)
                     /locus_tag="PK198_15445"
     CDS             complement(935..1951)
                     /locus_tag="PK198_15445"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HRF40185.1"
                     /translation="MNTIVRFLSPALIALLAIFNTAHAQQTPAVPTLNGFQLVDLALP
                     LEEMFIGGPSRESIKSIDKPEFSAAKDDDFLAANDLVIGITFNGVTKAYPARILNHHE
                     VVNDRFGDKSVAVTYSPLTGTAIAYLADVDGETRKFGVSGLVYNNNSLFFDTKTESLW
                     AQVLGQGVAGAASGQMLEQLPATWTTWNNWKGQNPNTLVLNRNTGHEVDYAVNPYEGF
                     ETTRGLRYPINKVNKMMPLKERVVGVEVNGTFRAYPMFVLQMENRPLIADTFNGVELL
                     IRYDKESKTVLVTNIKGEVYPSVTGYWYSWYAFHPETDVFMLNDGKNNRKPGEVTITT
                     ASIQ"
     assembly_gap    2266..2431
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2494..2700
                     /locus_tag="PK198_15450"
     CDS             2494..2700
                     /locus_tag="PK198_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40186.1"
                     /translation="MKFLYMFAVIFGFFGIGFILINLRHIVTGKEFRGTCASNNPMLK
                     DKVGECTVCGRMPGEECKGEEARA"
     gene            2759..>2957
                     /locus_tag="PK198_15455"
     CDS             2759..>2957
                     /locus_tag="PK198_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40187.1"
                     /translation="MYFFLVITLIGVVGLLLLMRVMADFQPNERKVKADLQKMRDEIA
                     QYGGELAPIDKQELDLFAVEQV"
     assembly_gap    2958..2980
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2981..3439
                     /locus_tag="PK198_15460"
     CDS             <2981..3439
                     /locus_tag="PK198_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40188.1"
                     /translation="KKRAVLTAKGVFTTIYHEPVAAYSYRRYVSSKLNALLYVRTAER
                     EFVYRFKGKEVQIILNNQPLGTIKENGVLYSARDGSMLARLNAETAGLLPVVGKDREL
                     GSISARNLPAAAKPGLNSRAFEFVNDNLNDQEETLFLSFALMELVKRSVG"
     gene            3766..>4314
                     /locus_tag="PK198_15465"
     CDS             3766..>4314
                     /locus_tag="PK198_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRF40189.1"
                     /translation="MSLPDKRKGYIVRREWTWERSIRKTEIPQFKEGGIEQAVLLPPK
                     NKETIPYLSIREKTDPWKDVHHSQGQMLAGEVINMRYSAVYVQLSPGIVGILRAEEMP
                     LLPGEYPKDVLSLGDILLVEILSVDGAARRIELSALKPLQRAMSSEQLSAELHQAFEG
                     SLRLMHERTQSLLSGSPVKAMRE"
     assembly_gap    4315..4326
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4327..>4695
                     /locus_tag="PK198_15470"
     CDS             4327..>4695
                     /locus_tag="PK198_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40190.1"
                     /translation="MAPLTRLNRILVIDNEQKEGEGVCGLLQRSLQAETVWVDTEKKA
                     LDAVSQQESKFDLAIIDINLSNGENGVRAAQKILAIAPTLPVLFMSSDPLSESRLLSL
                     ENDTHRRFPFALKDWRPQSET"
     assembly_gap    4696..4862
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5087..5101
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5102..>5424
                     /locus_tag="PK198_15475"
     CDS             <5102..>5424
                     /locus_tag="PK198_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40191.1"
                     /translation="YGLPVKMFGRTSEYALFAVDKRPDLSWNTISRIRLAAAYCGLTL
                     EKERFFAGLRPVQDLALRGELMSSFLHEFNNMLTPLFELMEDGAMLKQEGRKEELWSL
                     IENTA"
     assembly_gap    5425..5599
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5664..>5864
                     /locus_tag="PK198_15480"
     CDS             5664..>5864
                     /locus_tag="PK198_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40192.1"
                     /translation="MAEQNERFDQMNERKGASEFRQLQAHRLVKISTCASQQQNACCI
                     VVTDAGPGIPYHARERIFQMGYS"
     assembly_gap    5865..5982
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6012..>7161
                     /locus_tag="PK198_15485"
     CDS             6012..>7161
                     /locus_tag="PK198_15485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40193.1"
                     /translation="MAESKLLRVLLADDNPEVRNSVERRLSKRGVNVQTFSNARELLA
                     HLHTLSPDDMPTAVITDYVLPDMSGDQLVREILTSYRQLPLIVVSGEDMRGSIRSYGL
                     GAYAVMQKPLDYNELIVILRELSETDQVAMEIAGSLKDITEFGTCLVWELDRKDYPNY
                     RITGWSGCDREFASTTIMWENKYPRITKLKKGKALFIEDVHKVDYFIDKKAAEARGWV
                     SLISLPMMRQGKLIGWIDCYKNEQHDFKNEQRKKHQLVYLLNYTKLAAQALYARQLKQ
                     YSRVVHETQQNLAGALQESLIYNTILEKALATTGADYGWIYRYNPEGKYIELGAAAGP
                     GADKVEKKRYLTKGGITGKVADKGISLYVRDVDQPEDAYSPDVQVNIKG"
     assembly_gap    7162..7338
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7339..>8138
                     /locus_tag="PK198_15490"
     CDS             <7339..>8138
                     /locus_tag="PK198_15490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRF40194.1"
                     /translation="LQGISKLAAETSDFERLAQYVVDAVHDLTDADVNLWLMSAEENE
                     GDHYLRLRQTSIHERRGEDIKIPTAPGSSIDAVALQKKRFQIVDNIRDYSGEPGFVDK
                     EALLGYNWNSFMTVPLLGKQGEALGVISTMSREVNRFTEEEGNLVQHFANQAALALQE
                     QRHIRILQELANTGEQLTVGRSDAKALLDKVVQFARQVTHADLTVLYPYDPVKKLYYD
                     DESIVFSGTLKDGQKIPSQKPRENGMAAFVRRYEAMIVEYIPDMGELE"
BASE COUNT         1891 a         1736 c         1860 g         1607 t
ORIGIN      
        1 ccggctttac ccactcaccc tcgccgtcag caaacaactg atgccggtgt acgacaagcc
       61 catgatttat tacccgcttt cgaccctgat gtcggcgggc atccgcgata ttctcttcat
      121 ttctaccccg cacgatctgc cgcattttga aaaactgctc ggcgacggca gcgaaatagg
      181 ctgccatttc tcgtatgtgc cgcagtacgt tcccaacggg ctggcacagg cattcgtttt
      241 gggggcggat tttatcggtt ccgacagcgc cgcgcttatt ctgggcgaca atatttttta
      301 cggcaacggc ctcagcgagc aactccgcaa cagcgtcaac cccgatggcg gcgtcatctt
      361 cgcctatcag gtggccgacc cggagcgcta cggcgnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnccga tgtgaacaag cattatttag aaatgggccg
      601 cctgcatgtg gaggtattgg gccggggcat tgcctggttg gacacgggta cgcacaagtc
      661 gctgatgcag gccggtcagt tcatcgaagt catcgaagac cggcagggcc tgaaaatcgg
      721 ttgcattgag gaggtcgcct acgatatggg ctttattgat gcggcagagt tggagcggct
      781 ggggcataag tatcttaaaa gtgggtatgg ggagtatttg ctggggctat tgaannnnnn
      841 nnnnnnnnnn nnnnaaaagg ccggacactc gcaaggagtg cccggccaaa gcgcgttggt
      901 tgtatgtaat atcttgacga tcagattaaa atcatcactg aatcgaagcg gtggtgatgg
      961 tgacttcgcc cggtttgcgg ttgtttttgc cgtcgttgag cataaacaca tccgtttcgg
     1021 ggtggaaagc gtaccaagag taccagtagc ctgtgacgct gggatacacc tcgcctttga
     1081 tgttggtcac caagacggtt ttgctttcct tgtcgtagcg gatgagcagt tccacgccgt
     1141 tgaacgtgtc ggcaatgagc gggcggtttt ccatttggag cacaaacatc gggtaagcgc
     1201 ggaaagtacc gttgacttct acaccgacta cgcgctcttt cagcggcatc attttgttca
     1261 ctttgttgat cgggtagcgc aggccgcgag tggtttcaaa accctcgtaa ggattgacgg
     1321 cgtagtccac ctcatggccg gtattgcggt tgagtaccag cgtgttgggg ttctgacctt
     1381 tccagttgtt ccaggtagtc caggtggcgg gcagttgctc cagcatttgg ccggaagcgg
     1441 cgccggctac tccctgaccc aacacctgcg cccagagcga ctcggttttg gtgtcgaaaa
     1501 acagagagtt gttgttgtac accaagccgg agacgccaaa cttgcgcgtt tcgccatcca
     1561 catcggcgag gtaagcaatg gcggtgccgg tgagcggcga gtaagtaacg gccaccgact
     1621 tgtcgccgaa gcggtcgttg acgacctcgt ggtggttgag gatgcgggcg ggataagcct
     1681 tggtgacgcc gttgaaagta atgccgatca cgaggtcgtt ggcggcgagg aagtcatcgt
     1741 ctttggcagc cgagaactcc ggcttgtcaa tggatttgat gctttcccgc gatggacctc
     1801 cgatgaacat ttcctccaaa ggcaacgcta agtccaccaa ttggaagccg ttgagcgtgg
     1861 gtacggcagg cgtttgctgc gcgtgagcgg tgttgaaaat ggccagaagg gcgatgagcg
     1921 ccggcgagag aaaacggaca atagtgttca tagtaaaaca gatttaagag ttaaacatga
     1981 atgagatgat gaatgagcaa tttgttttgc tgtacatact caaccagaaa tcgtgccaaa
     2041 aatgaaatat gaaaagaagt gatttacaag taattgattt tcaagggtaa aatttttata
     2101 aaaaatattt tcgcgactat tcagtggctt tcccaagaag ccatgagccg cgaccacgcc
     2161 gaggcgtggc gcgagtttga tctcccaaaa tgaaatatgg ccttttttca agatgagatt
     2221 ttgaattttc ccaaaatgga aaatctaatt tctgaacgag aaaacnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacccttcc gctgccgccg ttgttggtaa
     2461 gtttgtttac ttttgcgatc aaattcaaat gcaatgaaat tcctctacat gtttgccgtc
     2521 atcttcggct ttttcggtat cggctttata ctcatcaatc tgcgccatat tgttaccggt
     2581 aaagaattca gaggcacctg cgccagcaac aatcccatgc tcaaggacaa agtcggagag
     2641 tgtaccgtgt gcgggcgcat gccgggcgaa gaatgtaaag gcgaagaagc cagagcttga
     2701 tctattccta acagcccgta actcgcggct cgcagctcaa agtaaacact ttccaaccat
     2761 gtactttttc cttgtcatca ccctcattgg cgtcgtaggt ttgctcctgc tcatgcgcgt
     2821 catggccgac ttccagccca acgagcgcaa agtgaaagcc gacctccaga aaatgcgcga
     2881 cgaaatagcg cagtacggcg gcgagctggc gcccatagac aaacaggaac tcgacctttt
     2941 tgcagtagaa caagtacnnn nnnnnnnnnn nnnnnnnnnn aaaaagcgcg ccgtactcac
     3001 ggccaaaggc gttttcacca ccatctacca cgagccggtg gcggcctaca gctacaggcg
     3061 ctatgtatcg tccaagctga acgcgctgct gtatgtgcgc accgccgaac gcgagtttgt
     3121 ctatcgcttc aaaggaaaag aggttcagat catcctcaac aaccagccct tgggcaccat
     3181 caaagaaaac ggcgtactct acagcgcccg cgacggcagt atgctggccc gcctcaatgc
     3241 cgaaacagca gggctgctcc ccgttgtcgg caaagaccgc gaattgggca gcatctcggc
     3301 cagaaacctg cctgccgccg ccaagcccgg cctcaattcc cgcgccttcg agtttgtcaa
     3361 cgacaacctc aacgatcagg aggaaacgct gtttttgtcc tttgcgctga tggaattggt
     3421 aaaaaggtcg gtggggtgag atactcggta gcgaagcctt taactttagg tttctacggc
     3481 gtagtgaatg acgggcgact acaagctgtg cttcaggcaa gggcctccct tcaccaacta
     3541 gcccccgcaa agtggcaggt tgttgttcag acaattcccc cgctatatcc cccgatttaa
     3601 aaaaaatact tctttcaggc gccttgttat caaaatgtaa tgtggagtga ttatatttgg
     3661 ccgttaaaca gccggtatca tttcatgagc gacacgaccc ataatcatca aggatacccc
     3721 atcttgcagg taagggtact caacatacgt cacaacagga tggtcgtgag cttgcctgat
     3781 aaacgaaaag ggtatattgt acgcagggaa tggacttggg aaagaagcat cagaaaaacc
     3841 gaaatacctc aattcaagga aggaggcatc gagcaagccg tattactgcc ccctaaaaac
     3901 aaggaaacca tcccctatct cagcatccgg gaaaaaaccg atccgtggaa agacgttcac
     3961 cacagtcagg gacaaatgct cgcaggcgaa gtcatcaaca tgcggtactc ggcggtttat
     4021 gtgcaattaa gccccggcat tgtgggcata ttgcgggcag aggaaatgcc cttgttgccc
     4081 ggcgaatacc ccaaagatgt gctgtctctg ggcgatatac tcttggtgga aattttatcg
     4141 gtggatggcg ccgcacgccg tatagaactg agcgccctga aacccctgca aagagccatg
     4201 agcagcgaac aactctccgc agaactgcac caagcatttg aaggcagcct cagattgatg
     4261 catgaacgga cgcagtcgct tttgtcaggc agccctgtga aggccatgcg ggagnnnnnn
     4321 nnnnnngtgg cgccattgac ccgcctgaat cgtatattgg tcatagacaa tgagcagaaa
     4381 gaaggagaag gcgtatgcgg actgctccaa cgcagcctgc aagctgaaac cgtgtgggtg
     4441 gacaccgaaa aaaaggcatt ggatgccgtg agtcaacaag aatcgaaatt cgatctggca
     4501 atcattgaca tcaatctctc taatggcgaa aacggtgtcc gcgcggcgca gaaaatatta
     4561 gcaatcgctc caactttgcc ggttttattt atgagcagcg accctctttc agagtcacgc
     4621 ctcttatcgc tggaaaacga cacgcatagg cgtttccctt ttgccttgaa agactggcgc
     4681 ccccaatctg agacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nngcgcagat cgatcactgc ctgctcctgc gattggacag cgaccaaaag gaagtatcgc
     4921 ttgcagcgtt ttatcctcct caaaatgaaa acatatacct ccgcagcatg gacggtctat
     4981 attacagtcc agtgcgcgaa gtcattgagg acggcgatat aagctacatg gccaatatcg
     5041 gcgaagcttc cgggcctcca gtttcggaga atttctttca aggcttnnnn nnnnnnnnnn
     5101 nctacggtct tcccgtgaaa atgttcggac gcacttctga gtatgccttg tttgcagtgg
     5161 acaagcgccc cgacctgtcc tggaatacca tctcccgcat ccggcttgcc gcggcctatt
     5221 gtggccttac cttggaaaaa gagcgctttt ttgcaggcct ccgtccagta caagacttgg
     5281 ccctgcgtgg tgagttaatg agcagcttct tacacgaatt caacaacatg ctcaccccgc
     5341 tcttcgagtt gatggaagac ggcgcgatgc taaaacaaga aggaagaaaa gaagaattgt
     5401 ggtctctgat agaaaatact gcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnna actgccattg cgctgcacgc ggagcaggta ctcacgaaca
     5641 tagtactcaa cgctatacaa aatatggcgg aacaaaacga gcgtttcgat cagatgaacg
     5701 aacggaaagg cgcatccgag tttcggcaac tacaggcgca tcgcttagtt aaaataagca
     5761 cttgcgcctc tcaacaacaa aacgcctgct gtattgtggt gacagatgcc ggccccggca
     5821 taccctatca tgcacgggag cgcatttttc agatggggta cagcnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatttttt cagctcaatt
     6001 aaaccatgat catggctgaa tctaaactcc tacgggtatt gctggcggat gataatcccg
     6061 aagtgcgcaa cagcgttgag cggcgcttgt ccaagcgcgg tgtgaacgtg cagacgtttt
     6121 ctaatgcccg ggaattgttg gcacatctcc ataccctttc gccagatgac atgccgacgg
     6181 cggtcattac tgattatgta ctgccggata tgagcggaga tcaattggtt agagaaatac
     6241 tcacttccta tcgccagcta ccgctgatcg ttgtgtccgg agaggatatg cgcggcagca
     6301 tccgaagtta cggattgggc gcttatgctg tgatgcaaaa accactggat tacaacgaat
     6361 tgatagtgat attaagagaa ttgtcggaaa ccgatcaggt ggccatggaa atagcaggaa
     6421 gcctgaaaga cattaccgaa tttggcactt gtttggtatg ggaattagat agaaaagatt
     6481 atcccaacta ccgcatcaca ggttggtcag gatgtgatcg ggaattcgcc tcaacaacca
     6541 tcatgtggga aaataaatat cctcgtatta ccaagctaaa aaaaggaaaa gcacttttta
     6601 tagaggatgt tcataaagta gattacttca tagataaaaa ggccgctgaa gcaagaggct
     6661 gggtatccct gatctctctg cccatgatgc gtcaaggtaa gctgatcggc tggattgact
     6721 gttacaaaaa cgagcaacat gatttcaaaa atgaacaaag aaaaaaacac caattagtct
     6781 atttgttgaa ttatacgaag ttggcggctc aggcattgta tgcccgacaa ttaaagcaat
     6841 actccagagt tgtacatgaa acccagcaaa acctggcggg agcacttcag gaatctttga
     6901 tatataacac cattttggaa aaagccttag ctacgacggg agcggattac ggttggatat
     6961 acaggtataa ccctgaagga aagtacattg aactgggcgc cgctgccggc ccaggcgctg
     7021 ataaagtcga aaagaaaagg tacctgacca aaggcggcat caccggcaaa gtagctgaca
     7081 aggggatttc actgtatgta agagacgtag atcaaccaga ggatgcttac agcccggacg
     7141 tacaggtaaa tatcaaaggg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnct gcagggcatc agcaaattgg cggccgaaac ctccgacttc
     7381 gagcgtttgg ctcagtatgt ggtggatgcc gtacatgatc tgacggatgc agatgtcaat
     7441 ttatggctga tgagtgcaga agaaaacgaa ggtgatcatt atctgcggct taggcaaacc
     7501 agtattcacg agcgcagagg cgaggatatt aaaataccca cagcccccgg cagcagcata
     7561 gatgcagtag ccctacaaaa gaagcgtttt caaattgtgg acaatatcag ggattacagc
     7621 ggcgaaccag ggtttgttga caaagaagca ttgctggggt acaactggaa ttcttttatg
     7681 accgttccac tcctcggcaa gcaaggcgag gcactcggcg tcatcagcac gatgagcagg
     7741 gaagtgaaca gattcacgga agaggagggc aatttggtgc aacattttgc caatcaggcc
     7801 gcgctggctc tacaagaaca aagacatatc cgcatattac aagaattggc caacaccggc
     7861 gaacaactca ccgttgggcg gtccgatgca aaggcattgc ttgacaaggt cgttcagttc
     7921 gcccgccagg tgacccatgc cgacctgacc gtgctctacc cttatgatcc tgttaaaaaa
     7981 ctctattacg acgacgagag cattgtgttc agcggaacac tcaaagacgg gcaaaagata
     8041 cccagccaaa aacctcgcga aaacgggatg gcagcttttg taaggcgata tgaagcgatg
     8101 atcgtggaat acatacctga catgggcgaa ctggagat
//
LOCUS       DAORLF010000204         8008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4879_length_8008_cov_2.087891, whole genome shotgun sequence.
ACCESSION   DAORLF010000204 DAORLF010000000
VERSION     DAORLF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_8008_cov_2.087891"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>313)
                     /locus_tag="PK198_15495"
     CDS             complement(<1..>313)
                     /locus_tag="PK198_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40195.1"
                     /translation="HAFPRSRGGFGSIDLDSLADGRDIFWPTNADSLRHANSDAHAIR
                     AEDAAENSNRGNRFYGQYSGPAGNMGSFKGDMARSVLYIAVRYNGLDVVPGYPEGMVG
                     L"
     assembly_gap    314..369
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<370..>854)
                     /locus_tag="PK198_15500"
     CDS             complement(<370..>854)
                     /locus_tag="PK198_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40196.1"
                     /translation="AQDITILFSLSNGTFGPADFSGDTSVVIPAGQLSATTTITILDD
                     ADDEGDEVAVFRLAPLPAEFLILNNNRPVRIVDNDFRVAPFGTPLQPSFGIVTGTQPD
                     NYYETLNGLSGPELRQALQNIIANPSVVRAQTYADLVDMLKEADQNPLNSNQVWLVYT
                     E"
     assembly_gap    855..1000
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1001..1543)
                     /locus_tag="PK198_15505"
     CDS             complement(<1001..1543)
                     /locus_tag="PK198_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40197.1"
                     /translation="MNRIIMAALLSFGAYLTTAQTLVINELDCDTPGIDDKEFVELRS
                     ETPHFPLDGYVLAFFNGSAAGANSSYMVFDLAGYTTDINGLLLIGSATVVPSPQYLIA
                     PNLIQNGADALAIYQRSVEDFPEGTLAFVDQTLIDVLIYGTNDADATGLISIFQAFRP
                     GIQQANEGPGNNTASIQRNNN"
     gene            complement(2079..2624)
                     /locus_tag="PK198_15510"
     CDS             complement(2079..2624)
                     /locus_tag="PK198_15510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF40198.1"
                     /translation="MTFIIYDLEATCWEGRPPTVVQEIIEIGALKLDDYGDVEDSFNA
                     FIKPVIHPQLSHFCQKLTSITQVDVNRASTFPKVIERFTEWIGVDEGEDYLLCSWGGF
                     DKRMFIQDCQLHRLEHDWVQAHINLKDQYHQFKRLNKARSLRHVVELEGFEFTGTYHR
                     GISDAENLAKVFVKYLDVWQY"
     gene            complement(2660..>3752)
                     /locus_tag="PK198_15515"
     CDS             complement(2660..>3752)
                     /locus_tag="PK198_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF40199.1"
                     /translation="PSFSYGAGLNLYNTGYTAYFMNRLGAYRVDRRKKNPIYLETLKS
                     MSNLSIQRGTNSLFFPGGTRSRSGALETKLKMGLLGTVVEAQRALYEKGENTKIFVVP
                     AILGYHFVLEAPYLVEQYLRQVGKERYIKSQDAFYSVTQLLKFTWQFFSQSSDINVSF
                     GQPMDVLGNPVDAEGMSYDQHGNPIDVREYFLGGESVNEDLQRETEYTKILADRIVER
                     YHKDNLVLSSHLVAFAAFQLLKRQNPRLDLYGILRLPVDEYIFSTEALTDVVGQLQQL
                     LIELHTDGHIQLSDILYATPEEIIQHGIRHLGAYHVDKPLRYNKAGEIVSDSFNLLFF
                     YHNRLSTYGWENHIQWRKRAMEEVAPGDD"
     assembly_gap    3753..3849
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3850..4373)
                     /locus_tag="PK198_15520"
     CDS             complement(<3850..4373)
                     /locus_tag="PK198_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HRF40200.1"
                     /translation="MSSSSKIYQHVIPDIENWPIFRLSEDRRSFVAEVQEFTVQRLLE
                     HGTPEVTDMLSKTVFQERVRIKEEPWKVDPPNERQFWRRIRKRLIQHSLDAPNEEATQ
                     NNEEILQLIVNRYAEEIVGTFRIGTFLFARRFLTLFFTRLLNTAAGGSLFGSKYRLQD
                     RLLVTGRTEAVRGL"
     assembly_gap    4423..4432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4476..>5698)
                     /locus_tag="PK198_15525"
     CDS             complement(4476..>5698)
                     /locus_tag="PK198_15525"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40201.1"
                     /translation="SIKELAKLLYEHHKAPVLLLIDEYDTPLVDAWLKGYYNETIEFI
                     RPLLASVFKDNDDILFKGVLTGILRVAKESLFSGLNNFVAEAGLEGDDFRDKFGFTEP
                     EVKALLVHRGLNGREMDDVRTWYDGYRYGSTPIYNPWSILNYAFAMEPSLRPHWVNTG
                     GHDLLKRLFFNEQSGIKDKLEELLRGGAIELYVDEFLTFRGLETNPDAVVSLMYFAGY
                     LRVVGEFRRGDRIIRQLSIPNREVRLAYEDTLMAWFNEDLGRSFNDTLLEALLAGQVQ
                     AFGAYLADFVVKVVSFYDTAKGRAEHFYHAFLLGLLARLDAKYRIRSNRESGYGRYDI
                     CLIPKNASQRGIVMEIKSPAIGSKETLEDCIVAARTQVLDKKYDAELTAHGVTDILRL
                     AIAVEGKELIVEQV"
     assembly_gap    5699..5712
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5713..>6070)
                     /locus_tag="PK198_15530"
     CDS             complement(<5713..>6070)
                     /locus_tag="PK198_15530"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40202.1"
                     /translation="RAVVDGAGVQLHCRPRRFGKTLNLSTLRYFFDCQGEYRHLFEGL
                     TIADDEEMTTRYQGKYPVITLSFKDVKMGSYTSTILGMGRLLYWEWRRHQHLEGRPDY
                     DRKIILMREAAEAGEPR"
     assembly_gap    6071..6118
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6213..>6410)
                     /locus_tag="PK198_15535"
     CDS             complement(6213..>6410)
                     /locus_tag="PK198_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40203.1"
                     /translation="AKGPYPPFKRTQRWVNIVLWVFFILFLLNTVGNLLAKTNFEKWF
                     ALVTLLLAGLIGNLLFRRTKK"
     assembly_gap    6411..6532
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6852..7166
                     /locus_tag="PK198_15540"
     CDS             6852..7166
                     /locus_tag="PK198_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF40204.1"
                     /translation="MNLRRDVFQAIADPTRRAILLMLAAQSMSAGAIAASFDSARSTV
                     SRHIHILTECELVQQDQQGREIYYHLNPLKMKEVVDFIEPFRQMWEERFDKLEDIMKK
                     QI"
     gene            7169..7633
                     /locus_tag="PK198_15545"
     CDS             7169..7633
                     /locus_tag="PK198_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF40205.1"
                     /translation="MELKTKIHTEEGKQDLLITRHFDLPPALLFRAFVEPDIVAQWMG
                     TSVLRLEARKFGAYQFETTDPKGHKHRFSGVIHEFVPEVKITRTFEMENTPFPVQLEF
                     LEFEKLSDNSSRLSMQIVYKSLEHKNQMLQLPFAQGINMAHNRLQEIVQSLK"
     gene            7640..>8008
                     /locus_tag="PK198_15550"
     CDS             7640..>8008
                     /locus_tag="PK198_15550"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF40206.1"
                     /translation="MTTDVATYLSEGCGRCSLGGTPECKVHTWQQELTELRRIVLECG
                     LDEECKWGVPCYTFQGKNIIMVSALKDGAIISFFKGSLLQDEPGLLSFAGENSQVAKI
                     ARFTRAEEILTVEELLKTYIF"
BASE COUNT         1772 a         1909 c         2045 g         1789 t
ORIGIN      
        1 aacagcccca ccatgccttc ggggtagccg ggcactacgt ccaagccgtt gtagcgcacc
       61 gcgatgtata aaacactgcg ggccatatcg cccttgaaac tgcccatgtt gccggcggga
      121 ccgctgtatt ggccgtagaa acggttgccg cgattgctgt tttcagcggc gtcttcagcg
      181 cggatggcgt gggcatcgga attggcatgg cggagggagt cggcattggt gggccagaaa
      241 atgtctctgc cgtcggccag cgagtcgagg tcaatactgc cgaagccgcc gcgcgagcgt
      301 gggaaggcgt gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnng ttcggtatag acgagccata cctgattgct gttcagcggg ttttggtcgg
      421 cctctttcag catgtccacg aggtcggcgt aggtttgagc gcgtaccacc gatgggttgg
      481 caatgatgtt ttggagcgcc tgccggagtt ccgggccgct gagtccgttc agggtttcat
      541 aataattatc tggttgcgtg cctgttacga tgccgaagct gggctggagc ggcgtaccga
      601 aaggcgctac ccggaagtca ttgtccacga ttcggacggg gcggttgttg ttcagaatca
      661 gaaactcggc gggcaggggc gccagtcgaa acacggccac ctcgtcgcct tcatcgtcgg
      721 cgtcgtccag aatggtaatg gtggtcgttg cggaaagctg gccggcagga atcactacgc
      781 tggtatcgcc gctgaaatcg gcagggccga atgtgccgtt gctgagcgaa aaaagaatcg
      841 ttatgtcttg agcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttgttgttg cgctgaatgg
     1021 aggcggtatt gttgcccggc ccttcgttgg cttgttgaat gccggggcgg aatgcctgaa
     1081 aaatggaaat cagcccggtg gcgtcggcgt cgttggtgcc gtaaatgagt acgtctatca
     1141 atgtttggtc aacaaaagcg agcgttcctt cgggaaaatc ctccacactg cgctgataaa
     1201 tggccagagc atcggcgccg ttttggatga ggttgggggc aataagatat tgcggtgaag
     1261 gcactaccgt ggcgctgccg atgagcagca ggccgttgat gtcggtggta taaccagcca
     1321 gatcgaaaac catgtagctg gaattggcgc ccgccgccga accgttgaag aatgccagca
     1381 catatccgtc aagcggaaag tgaggcgttt ctgagcggag ttctacaaac tctttatcgt
     1441 caatgccggg cgtgtcgcag tccaattcat tgataaccaa tgtttgcgcg gtggtgagat
     1501 aagccccgaa cgataagagg gccgccatga taattctgtt catgaatgct gtattttgcg
     1561 caaagatgcc cttttttcgg cacttgcccg atacggcgta gcttcctgtc ggctgtgttg
     1621 cgcataaaat aaggggcctg tcgccgccgc tggccgatta tggcgaaaaa gatttttttt
     1681 gcagaacaat tgcatgagaa ataaaaatac tcctatattg cctcccgtta caaaaacagc
     1741 aggtttctgg cctccatttc attcaaaaag acgggcagca accatcaaaa acggcactat
     1801 tattatcttc ataataacag tcgggtgatt tcttataaga gcgaagcaat ttttattgtc
     1861 ttcgctcttt tttttggaag ttcaggctta tataaaaatg aacggcagcg ggtagaaccc
     1921 gccgaatagc ggcacaagat ggaatcttgc gccagcaggt ttgatcaaaa actacatcca
     1981 tccacccgct ttggcgcaat gagcagccga gagccgcacc cctcacctct cactcactca
     2041 cccctgctgc acccctcacc actcaccact tacccctgct aatactgcca cacatccaaa
     2101 tacttcacaa aaaccttggc cagattctcc gcgtcggaaa tgccccggtg gtaagtgccc
     2161 gtgaactcga agccctccaa ctccaccaca tggcgaaggc tgcgcgcttt gttgaggcgt
     2221 ttgaactggt ggtattgatc cttgagattg atatgcgcct gtacccagtc gtgctccaag
     2281 cggtgcaatt ggcagtcctg aatgaacatg cgcttatcga agccgcccca ggaacaaagc
     2341 aggtaatctt cgccctcatc cacgccaatc cattcggtga agcgctcaat gacttttgga
     2401 aaagtagaag cccggttcac atccacctga gtgatagagg tcagtttttg gcagaaatgc
     2461 gacaactgcg ggtgaatgac cggcttgatg aaagcgttga aggaatcttc cacatcgccg
     2521 tagtcgtcca atttcagggc gccgatttct ataatttcct gcactacggt gggcggtcgc
     2581 ccttcccagc aggttgcctc cagatcgtaa atgataaagg tcatgggatg ctgtgcttgg
     2641 cctctggttt tgcgccggtt cagtcgtcgc cgggcgccac ttcttccata gcgcgtttgc
     2701 gccattgaat atggttttcc cacccgtagg tggagaggcg gttgtgataa aaaaacaaga
     2761 gattgaaact atcgctgacg atttcgccgg ctttgttgta ccgcagcggc ttgtccacat
     2821 ggtaagcgcc gaggtgcctg atgccgtgct gaatgatttc ctccggcgtg gcgtagagga
     2881 tgtcggagag ttgaatatgg ccgtcggtgt gcaactcgat gagaagttgt tgcaattggc
     2941 ccaccacatc ggtgagggct tcggtggaaa agatgtactc gtccacgggc agtcggagga
     3001 tgccgtagag atcgaggcgc gggttttggc gtttcagcag ttggaaagcc gcaaaagcca
     3061 ccaaatgact gctcagcacg aggttgtctt tgtgatagcg ctctacgatg cggtcggcca
     3121 gaattttggt gtattccgtt tcgcgttgga ggtcttcgtt tacgctctcg ccgccaagaa
     3181 aatactcgcg cacatcaatg ggattgccgt gctgatcgta gctcatgccc tctgcatcca
     3241 cgggattgcc cagcacgtcc atcggctgcc cgaacgacac gttgatgtcg ctggattgcg
     3301 aaaaaaactg ccaggtgaat ttgagcaact gggtgacgct gtaaaatgcg tcctgcgatt
     3361 tgatgtaacg ctccttgccc acttgccgca ggtactgctc gacgaggtaa ggcgcttcca
     3421 gtacaaaatg gtatcccaga atggccggca ccacaaagat tttcgtgttt tcgccttttt
     3481 cgtacaaagc ccgctgcgcc tctacgacgg tgccgagcaa gcccattttg agcttggttt
     3541 ccaacgcgcc cgaacgcgag cgggtgccgc cgggaaagaa caggctgttg gtgccgcgct
     3601 gtatgctcag gttcgacatg ctcttgagcg tctccaggta gatggggttt ttcttgcgcc
     3661 ggtccacccg ataagcgccc agacggttca taaagtaggc cgtgtagcct gtattgtaaa
     3721 gattcagccc cgcgccgtag gagaacgaag gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnna acaagccccg cacagcctcg gtgcgccccg ttaccaagag gcgatcctgt
     3901 agccggtatt tactgccgaa gagactgccg ccggctgccg tattgagcag gcgggtgaaa
     3961 aaaagcgtga gaaaacgccg ggcaaacaaa aacgtgccga tgcgaaatgt acccacaatc
     4021 tcctctgcgt agcgattgac gatgagttgc aaaatctcct cgttgttttg ggtagcttcc
     4081 tcattggggg catctaatga gtgttgaatc aggcgtttgc gaatgcgtcg ccaaaattgc
     4141 cgctcattgg gcgggtccac tttccagggt tcttctttga tgcgcacccg ctcctgaaac
     4201 acggttttcg agagcatatc ggttacttcc ggcgtgccgt gttccagcag gcgttgcaca
     4261 gtaaattcct gcacctcggc tacaaaactt cggcggtctt cactgagccg gaagatgggc
     4321 cagttttcaa tgtcgggtat gacgtgctgg tatattttgc tgctgcttga catgaaaggc
     4381 gtgcgttagg gaatgtaaag gtagtaaaac tgcgataaac cannnnnnnn nngattccgc
     4441 gcgaagtgca gatgaactgg cgaagccgat gaccgctaaa cttgctctac gatcagctcc
     4501 ttcccctcca ccgcaatggc caatcgcagg atgtccgtca cgccgtgagc cgtgagttcc
     4561 gcgtcgtatt ttttatctaa tacctgagtg cgggcggcga caatgcagtc ttctaaggtt
     4621 tctttgctgc ctatggccgg cgattttatt tccatgacaa tgcctctttg ggaagcattt
     4681 ttgggaatca gacaaatgtc gtaacgcccg tagccgcttt cccgattgga gcggatgcgg
     4741 tacttcgcgt ccaaacgggc cagcagcccc agcaggaagg cgtggtagaa atgctcggcg
     4801 cggcccttgg cagtatcgta aaaactgacc accttgacta caaaatccgc gaggtatgcg
     4861 ccaaaagcct gcacctgccc cgcgagcagc gcctccaaga gcgtatcatt gaaagagcgc
     4921 cccaaatcct cgttgaacca agccatgagg gtgtcttcgt aagccaacct cacctctctg
     4981 ttggggatgg atagttgtcg tatgatcctg tctcctcgcc tgaactcgcc caccacccgc
     5041 agatatccgg caaagtacat caagctgacg acagcatcgg ggttggtctc taagccgcga
     5101 aaagtgagaa actcatccac atacaactca atggcgccac cgcgcaacag ctcttccaat
     5161 ttgtctttta tgccagactg ttcattgaaa aagagccgtt tgagcaggtc gtggccgccg
     5221 gtgtttaccc agtgtggcct taggctgggt tccatagcga aggcatagtt gagtatggac
     5281 caggggttat aaatcggggt gctgccgtat cggtagccgt cgtaccaggt gcgcacatcg
     5341 tccatctcgc ggccattgag gccccgatga accagtaggg ccttgacctc cggctcggtg
     5401 aagccaaact tgtcacggaa gtcatcccct tctaaacccg cctcggcgac aaaattgttc
     5461 agtccgctga ataggctctc tttggctacc cgcaggatgc cggtgagtac gcctttgaaa
     5521 agtatgtcgt cattgtcttt gaacacactg gctaatagtg ggcgtatgaa ttcaattgtt
     5581 tcgttgtagt accctttcag ccacgcgtcc accaatggtg tatcgtactc gtctatcagc
     5641 agcagcacag gggccttgtg atgctcgtac agcagcttgg ccaattcttt gatgctttnn
     5701 nnnnnnnnnn nncacgaggc tcgcccgcct ccgcggcctc ccgcatcaga attatcttac
     5761 ggtcgtagtc gggcctaccc tccagatgct ggtgccggcg ccactcccag tacagaagcc
     5821 tgcccattcc cagtatcgtg gaggtgtagc tccccatctt cacgtctttg aacgacagcg
     5881 taatgaccgg gtacttgccc tgataccggg tcgtcatctc ctcatcatcg gctatggtca
     5941 aaccctcaaa aagatgccgg tattcgccct gacagtcaaa gaaatagcgc agcgtggaga
     6001 ggttcagcgt tttgccaaag cgacgcgggc ggcagtggag ttgtacgcct gcgccatcca
     6061 ctacggcgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     6121 gatttctttg aagtctgacg aaccgagtgg cgagcgtttg ggcatgatgt atgcgtttta
     6181 ctgcaaagat acgggtttta caaaacgaaa attcatttct ttgttcgccg gaaaagcaga
     6241 ttgccgatca gtccggccag caacaaagtg acgagcgcaa accatttttc aaagttggtt
     6301 ttggccagta ggttgcccac tgtgttgagt aaaaagagga taaaaaacac ccacaataca
     6361 atgttgaccc agcgttgcgt gcgcttgaat ggaggatagg ggcctttggc nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccagaat
     6541 gcagaggtgc agggcaatga cgagtacgga gatgaccaga aggatttgga tggataaggc
     6601 gcggctcatg gttggataaa attggtagtg agaatggcga tgcaagatac gacaaattaa
     6661 attcgggatg aaaatcctga ttgtcttctt agcctcagct tgatatgcgc ttggctttgt
     6721 gcattttgtg atatatgtat cgaagcctta cgcattcgag gaacactccc gttgaatttt
     6781 tttcaaaaaa aattcaaaaa agatatgtcg ttaaataacg acgcgtatat ttgtcgtcaa
     6841 atagcgacat aatgaatttg agaagagacg tttttcaggc catcgccgat cctacccgcc
     6901 gggccatatt gctcatgctg gcggcacaat ccatgtcggc aggagctatt gcggcctcct
     6961 tcgactcggc ccgatccact gtttccaggc acattcatat ccttaccgaa tgtgagcttg
     7021 tgcagcaaga ccagcaggga agggagattt attatcatct gaatcctttg aaaatgaaag
     7081 aagtagtaga tttcatcgag cctttccgcc agatgtggga agaacggttt gataagttgg
     7141 aagacatcat gaaaaaacaa atctgacaat ggaactgaaa acgaaaatac acacagagga
     7201 aggaaaacaa gacttgctca ttacgcgcca tttcgacttg ccgccggcat tgcttttcag
     7261 ggcttttgtc gagccggata ttgtagcgca atggatgggt accagtgttt tgcgtttgga
     7321 agcccgaaaa tttggggcct accaatttga aaccaccgat ccgaagggcc ataagcatcg
     7381 gttcagcggg gtgatacacg agtttgtacc cgaagtgaaa atcacgcgca cttttgaaat
     7441 ggaaaacact ccgtttcctg tacaattgga atttctggag tttgaaaagc taagcgacaa
     7501 cagcagccgg ctcagtatgc aaattgtgta caagtcgctg gaacacaaaa accagatgct
     7561 gcaattgccc tttgcgcaag gcatcaacat ggcgcacaac cgtttgcaag agatcgttca
     7621 atcattaaaa tgaaaaaaaa tgaccaccga cgtagcaacc tacctctctg aaggctgcgg
     7681 gcgctgctcg ctcggcggca cgcccgaatg taaagtgcat acctggcagc aggaactgac
     7741 ggaattgcgg agaatcgttc tggagtgcgg cctcgacgag gaatgtaagt ggggcgtgcc
     7801 ctgctacacg tttcagggca aaaacatcat catggtttcc gcgctcaaag atggcgccat
     7861 catcagtttt ttcaaaggct ctctgttgca agatgagccg ggattgctga gtttcgccgg
     7921 cgaaaactcg caggtggcca aaatagcccg cttcacccgc gcagaggaaa ttttgacggt
     7981 ggaagaactc ctgaaaacct acattttt
//
LOCUS       DAORLF010000205         8001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4887_length_8001_cov_1.951674, whole genome shotgun sequence.
ACCESSION   DAORLF010000205 DAORLF010000000
VERSION     DAORLF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_8001_cov_1.951674"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PK198_15555"
     CDS             complement(<1..349)
                     /locus_tag="PK198_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRF40207.1"
                     /translation="MKRFITTLQNIWKIKELRNRILFTLTLLLIFRLGSYIVLPGVIP
                     AVLENQDNSNPNDLLGLINVFTGGAFNNAAVLALGIMPYITASIILQLLGFAVPYFQR
                     LQQKEGESGRRKLN"
     gene            complement(359..805)
                     /gene="rplO"
                     /locus_tag="PK198_15560"
     CDS             complement(359..805)
                     /gene="rplO"
                     /locus_tag="PK198_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRF40208.1"
                     /translation="MELHNLKPAKGAVKTRKRVGRGQGSGMGATSTRGHKGAGSRSGD
                     YTKVGFEGGQMPLQRRLPKVGFKSPNRIELVPLNVDRLQAIAEKFKTDTITPEFLVAN
                     GIVGKGDYVKVLGSGELTAKLNVAAHAFSATAKAAIEANGGSVHLV"
     gene            complement(819..>989)
                     /gene="rpmD"
                     /locus_tag="PK198_15565"
     CDS             complement(819..>989)
                     /gene="rpmD"
                     /locus_tag="PK198_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRF40209.1"
                     /translation="KVTQVKSTIDRPKRQKETIKALGIKKMHQSVVHEATPQILGMVN
                     KVNHLVKVEEVQ"
     assembly_gap    990..1113
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1114..1298)
                     /locus_tag="PK198_15570"
     CDS             complement(<1114..1298)
                     /locus_tag="PK198_15570"
                     /inference="COORDINATES: protein motif:HMM:NF015664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRF40210.1"
                     /translation="MKVPIHKGTIPHEQKGKFGAGKVLLKPASDGTGVIAGGAMRAVL
                     EMVGIHNVLAKSTGSSNP"
     gene            complement(1315..1521)
                     /locus_tag="PK198_15575"
     CDS             complement(1315..1521)
                     /locus_tag="PK198_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40211.1"
                     /translation="MDKRSNNKVKPVESTMKEKLVALNRVAKVTKGGRTFSFAAIVVV
                     GDENGTVGQGLGKARDVSESIAKG"
     gene            complement(1553..1897)
                     /gene="rplR"
                     /locus_tag="PK198_15580"
     CDS             complement(1553..1897)
                     /gene="rplR"
                     /locus_tag="PK198_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRF40212.1"
                     /translation="MNNLKRKKIHYRIRKKVRGTASRPRLAVYRSNKAIYCQAIDDVA
                     GHTLAAASSLEASVPGDVPKVEQSRMVGKLVAERMLAKNVSAAVYDRGGYLYHGRVKA
                     LAEGAREGGLTI"
     gene            complement(1967..>2191)
                     /gene="rplF"
                     /locus_tag="PK198_15585"
     CDS             complement(1967..>2191)
                     /gene="rplF"
                     /locus_tag="PK198_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRF40213.1"
                     /translation="IMFYVPSEVKVEAKQEKGKNPIIRLESHDKQLIGQIAAKIRAFR
                     KPEPYKGKGVRFVGEEIRRKAGKTAGKGKK"
     assembly_gap    2192..2215
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2216..2538)
                     /gene="rplF"
                     /locus_tag="PK198_15590"
     CDS             complement(<2216..2538)
                     /gene="rplF"
                     /locus_tag="PK198_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRF40214.1"
                     /translation="MSRVGKMPVSLPSGVEITVSKSNLVTVKGPKGTLSQQVHPDLIL
                     EMDNGVLEVKRPTDQKRHREVHGLTRALINNMVVGVSEGFVKELELVGVGYRASNETM
                     KQLLEL"
     gene            complement(2568..2978)
                     /gene="rpsH"
                     /locus_tag="PK198_15595"
     CDS             complement(2568..2978)
                     /gene="rpsH"
                     /locus_tag="PK198_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRF40215.1"
                     /translation="MAVTDPIADYLTRIRNAQQAGHKIVEIPASGVKKRMTEILYDQG
                     YILKYKFDDEAGQGGQGVIKIALKYDLASRKPVIRQLLRVSKPGLRKYSSAEELPRVI
                     NGLGIAIISTSKGVMTDKEARAQHIGGEVLCYIY"
     gene            complement(3036..3305)
                     /gene="rpsN"
                     /locus_tag="PK198_15600"
     CDS             complement(3036..3305)
                     /gene="rpsN"
                     /locus_tag="PK198_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRF40216.1"
                     /translation="MAKKSVIAREVKRAKMVAKYADLRKKLKEEGNWEELDTLPRNSN
                     PIRMHNRCGLTGRPKGYMRRFGICRVKFRQMALDGKIPGVTKASW"
     gene            complement(3315..3878)
                     /gene="rplE"
                     /locus_tag="PK198_15605"
     CDS             complement(3315..3878)
                     /gene="rplE"
                     /locus_tag="PK198_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRF40217.1"
                     /translation="MSTYIPRFKKKYREEVVPALMEQFQYGSIMEVPRLVKISLNQGI
                     GEATQDKKLVDNAAAELTLIAGQKAVATKAKRSVSNFKLREGMPIGARVTLRDHRMYE
                     FLDRLITVALPRVRDFRGINDKSFDGRGNYSMGVTEHIIFPEIDLDKVSKIMGMDITF
                     VTTAKTDQEAFALLKLLGLPFKNQKNN"
     gene            complement(3878..4228)
                     /gene="rplX"
                     /locus_tag="PK198_15610"
     CDS             complement(3878..4228)
                     /gene="rplX"
                     /locus_tag="PK198_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRF40218.1"
                     /translation="MAAKNQQKRFAPKLKIKKGDRVVVIAGAYKDRSKVREVLEVFPD
                     ENRAIVDQVNIVKRHTKPTQNSQGGIIEKPAPIHLSRLMLVDPKTGEPTRVGRKLEDG
                     KLVRYSKKSGETFK"
     gene            complement(4273..4638)
                     /gene="rplN"
                     /locus_tag="PK198_15615"
     CDS             complement(4273..4638)
                     /gene="rplN"
                     /locus_tag="PK198_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRF40219.1"
                     /translation="MIQQESRLRVADNSGAKEVLCIRVLGGSHRRYASIGDQIVVSVK
                     DASPGGIKKGTVSKAVVVRTKKEIRRKDGTYIRFDDNAVVLLNAQGEPRGTRIFGPVA
                     RELRERDYMRIVSLAPEVL"
     gene            complement(4648..4905)
                     /gene="rpsQ"
                     /locus_tag="PK198_15620"
     CDS             complement(4648..4905)
                     /gene="rpsQ"
                     /locus_tag="PK198_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRF40220.1"
                     /translation="MTERNLRKTKVGVVTSNKMDKTIAVSVERRLQHPIYGKFVKKSN
                     KFLAHDEKGECNIGDVVKIMETRPLSKFKRWRLVEIIERAK"
     gene            complement(4937..5200)
                     /gene="rpmC"
                     /locus_tag="PK198_15625"
     CDS             complement(4937..5200)
                     /gene="rpmC"
                     /locus_tag="PK198_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRF40221.1"
                     /translation="MASNKFLELQEFTDADLEGELAQLEVQYQKLRFDHALKGLDNPL
                     NIREMRRDIARVQTEIRRREVAAMDEAGLQSRSKIRTRRSKGK"
     gene            complement(5232..>5650)
                     /gene="rplP"
                     /locus_tag="PK198_15630"
     CDS             complement(5232..>5650)
                     /gene="rplP"
                     /locus_tag="PK198_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRF40222.1"
                     /translation="LQPKRTKYRKQQKGRNEGLDYRGSTIAFGSVGLKAVTGGRLTNR
                     QIEAARIAMTRYMKREGKVWIRIFPDKPITAKPQEVRMGKGKGSLDHWVAQIKPGRIM
                     FELDGVPYAVAAEALRLAAQKLPVMTKTITRPDYQE"
     assembly_gap    5651..5732
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5744..>6103)
                     /locus_tag="PK198_15635"
     CDS             complement(5744..>6103)
                     /locus_tag="PK198_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40223.1"
                     /translation="GAEMSRTEEFKDGRTPLHTFRADIDYALGEAQTVYGKIGIKVWL
                     CKGEVFGKRDLSPNIGVTKKETTPGGKERGMGGRPERGERRDGGRGDRRDGGRGRGGE
                     RGDRGEGGGGRRNQKRR"
     assembly_gap    6104..6129
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6130..6583)
                     /gene="rpsC"
                     /locus_tag="PK198_15640"
     CDS             complement(<6130..6583)
                     /gene="rpsC"
                     /locus_tag="PK198_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRF40224.1"
                     /translation="MGQKTNPIGNRLGIIRGWDSNWFGGKDFGQKVVEDEKIRTYLNA
                     RIAKGGIARIIIERTLKRITVTIHTSRPGIIIGKGGQEVDRIREELKKLTGKESQINI
                     VEIRKPEMDAAIVAESVAKQLEARINYRRAIKTAIQATMRAGAEGIKVR"
     gene            complement(6614..7009)
                     /gene="rplV"
                     /locus_tag="PK198_15645"
     CDS             complement(6614..7009)
                     /gene="rplV"
                     /locus_tag="PK198_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRF40225.1"
                     /translation="MEAVAKLKNCPMSARKMRLVMDNIRGKNVSQALGILRYTKRESG
                     SWLEKVLLSAIANWEYKLDNMENADDYELYIKTALVDEGTFLKRFQPAPHGRAHRVRK
                     RTCHATIVVENRKQLDSEVVTAQEAETEN"
     gene            complement(7082..>7346)
                     /gene="rpsS"
                     /locus_tag="PK198_15650"
     CDS             complement(7082..>7346)
                     /gene="rpsS"
                     /locus_tag="PK198_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRF40226.1"
                     /translation="RSIKKGPYIFHKLLDKVFKAKQSNKKAVIKTWSRASMIIPDMVG
                     ETIAVHNGKTFVPVYVTENMVGHKLGEFAPTRNFRGHSGNRKK"
     assembly_gap    7347..7385
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7389..>8001)
                     /gene="rplB"
                     /locus_tag="PK198_15655"
     CDS             complement(7389..>8001)
                     /gene="rplB"
                     /locus_tag="PK198_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRF40227.1"
                     /translation="GVPATVVSIEYDPNRSAFIALLQYEDGEKRYIIAPHGLKVGAVL
                     LSGSGVTPTIGNTMLLSEIPLGAEIHAIELQPGKGARSAGTYAVLTGREGKYASLRLP
                     SGEFRRVLLTCRATIGVASNPDHSLIVSGKAGRSRWLGRRPRVRGVAMNPVDHPMGGG
                     EGRASGGHPRSRNGKPAKGLKTRNNKKQSTKLIISRRKSSNKK"
BASE COUNT         1754 a         2006 c         1776 g         2170 t
ORIGIN      
        1 ggtttaattt acgacgacct gattcacctt ctttttgctg aagcctttga aaatacggca
       61 cagcaaaacc cagcaactga agaatgatgc tggcagtgat atagggcata attcccaatg
      121 ccaacacggc cgcattattg aaagcaccgc cggtgaagac gtttatcaac cccaaaaggt
      181 cattcggatt actattgtcc tgattctcca gaacagcagg aattacacct ggtaatacga
      241 tgtaagaccc taagcggaag atcagcaaaa gagtgagcgt aaatagaatc ctattacgca
      301 gctccttgat tttccagata ttttggaggg tggtgataaa tcttttcatt gcttgaattt
      361 aaacaagatg aacactacct ccattcgctt caatagcagc tttggcagta gccgaaaaag
      421 catgagcggc aacgttgagt ttggcagtca gttcgccact acccaacacc ttgacataat
      481 cgcctttacc aacgatgcca ttggccacca gaaattccgg ggtaatggta tccgttttga
      541 atttttcagc gatggcctga aggcgatcaa catttaaagg taccaactca atacggttgg
      601 ggcttttgaa gccgactttg ggcaaacggc gttgcagagg catctggcca ccttcgaagc
      661 ccactttggt ataatcgcct gaacgtgagc cggcgccttt gtgaccacgg gtcgaggtag
      721 cgcccatacc tgagccttgt ccccgtccaa ctctttttct ggtcttaacg gcgcctttag
      781 ccggttttaa attatgcagt tccatgtttt ggttgctttt attggacttc ctccactttc
      841 accaaatgat tcactttatt gaccatgccc agaatttgag gcgtggcttc atgcactaca
      901 ctttggtgca tcttcttgat tcccagcgct ttgatagttt ctttctggcg cttgggacgg
      961 tcaattgtac ttttgacctg agttactttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggttgg aagaccctgt tgacttggcc
     1141 aacacattgt gaatgcctac catttcaagc acagcacgca tagcgccgcc ggcaattaca
     1201 cccgtaccgt ctgatgccgg tttgagcaaa accttaccgg cgccaaactt gcctttttgt
     1261 tcgtgcggaa tggtgccttt gtgaatagga actttcacta agttcttctt ggcatcatcc
     1321 tttggctata gattccgaca catcgcgtgc tttacccaag ccctgaccta cggtgccgtt
     1381 ttcatcaccc acaaccacaa tggcagcaaa actgaaggta cgaccacctt tggttacttt
     1441 agcaacccgg ttgagcgcca ccagcttttc tttcatggtg gattctacgg gctttacttt
     1501 attgttgctt ctcttatcca tgcttgaaat gcaattgaat cattctttta gattagatcg
     1561 tcagaccgcc ttcgcgtgcg ccttctgcca gagctttcac tctgccatga tacaggtacc
     1621 caccacgatc atatactgcg gctgaaacat tcttcgccag cattctctca gctaccaatt
     1681 tacccaccat gcgcgattgc tctactttag gcacatcacc gggaacggat gcctctaaag
     1741 aggaggcggc agccaatgta tgaccggcta cgtcatctat agcctgacaa tagatggcct
     1801 tgttgctgcg atacaccgcc aatcttgggc gggaagccgt tccgcgaact tttttgcgga
     1861 tgcgataatg gattttttta cgcttaagat tgttcatttt caaatatttt ggctttagtg
     1921 tatggcctgt ttcatccaaa cccaaaaccc gacaatgatg aaatttttac ttcttaccct
     1981 taccagcagt cttgccggct ttgcgacgaa tttcttcacc cacaaagcga acacctttgc
     2041 ctttgtaagg ctctggcttg cggaaagcac ggattttagc cgcaatttga ccaataagct
     2101 gcttgtcgtg gctctctaac cgaatgatcg ggtttttgcc cttttcctgc ttagcctcca
     2161 ctttcacttc ggaaggaaca taaaacatga tnnnnnnnnn nnnnnnnnnn nnnnnagctc
     2221 caacaactgc ttcatagtct cgttggaagc gcgataacct acaccgacaa gctcaagttc
     2281 tttaacaaac ccttctgaaa ctccaacaac catattgttg atgagtgccc gggtgaggcc
     2341 atgtacctcc ctgtggcgct tttgatcggt tgggcgctta acctccaaaa ctccattatc
     2401 catttcaagg ataaggtcag gatgtacctg ctggctaagt gtgcctttgg ggcctttcac
     2461 cgtcaccaaa ttgctcttgc ttaccgtaat ttcaacgccg gagggcaatg atacgggcat
     2521 ttttcctaca cgagacatgg tatgattgtt ttaatacctt ttcaaaatca ataaatgtag
     2581 cagagaactt cgccgccgat gtgctgagca cgcgcttctt tatcggtcat tacacctttg
     2641 gaagtagaaa tgatggcgat tcccaagcca ttgataacgc gaggcaattc ttcggcagag
     2701 ctgtacttgc gaagacccgg cttgctgacg cgcagcaact ggcgaatcac aggcttacga
     2761 gaggcaagat cgtacttcaa tgcgatctta atcacgccct ggccgccctg accagcttcg
     2821 tcgtcaaatt tgtacttcag gatgtatccc tgatcgtaga gaatttccgt catgcgcttt
     2881 ttgacgccag aagcaggaat ttccactatt ttatgacccg cttgttgagc gttgcgaatt
     2941 cgggtcagat aatctgcaat aggatccgtt acagccattg tttaacaatt atacggatga
     3001 atcaacctca gttgaaatga atttgaattt atgaattacc agcttgcctt ggttacgccg
     3061 ggaatcttac catcaagtgc catctgacgg aacttcacgc ggcagatacc aaaacggcgc
     3121 atgtaacctt taggacggcc ggtgagacca cagcgattgt gcatgcgaat tggattgctg
     3181 ttgcgaggca atgtatccaa ctcttcccaa ttgccttctt ctttgagctt tttgcgcaag
     3241 tcagcgtatt tggccaccat tttggcacgc ttcacttctc tagcgataac tgatttcttt
     3301 gccatgagta ttgatcagtt gtttttctgg ttcttgaaag gcaggccgag caatttcaac
     3361 aaagcaaacg cttcctgatc ggtcttggcc gttgtgacaa aagtgatgtc catacccatg
     3421 atcttgctca ctttatcgag gtcaatttca gggaagataa tatgctcagt gacacccata
     3481 gagtagttgc cgcgcccatc gaagctctta tcattgatgc cgcggaagtc gcgtacacga
     3541 gggagcgcta cggtgataag gcgatccaaa aactcataca ttctgtgatc acggagggtt
     3601 acacgggcgc cgatgggcat accttcgcgc aatttaaagt tggacacaga acgctttgct
     3661 ttggtagcta ctgctttctg accggcaatg agcgtaagct cggcggcggc gttgtccacc
     3721 aattttttgt cctgtgtagc ttcgccgatt ccctgattga gggaaatttt caccaaacga
     3781 ggtacctcca tgatagagcc gtattgaaac tgctccatga gagcgggaac tacttcctcg
     3841 cggtactttt tcttgaatct cggaatatat gtgctcatca cttaaatgtt tcaccggatt
     3901 ttttagaata acgaaccaat ttaccgtctt ccagcttgcg acctacgcgg gtgggttcgc
     3961 ccgttttagg gtccacaagc atcagtcgag acaggtgaat cggggccggc ttttcgatga
     4021 tgccgccctg actgttttgc gtcggcttgg tgtggcgttt cacgatgttg acctgatcta
     4081 caatagcccg gttttcatca gggaacacct ccagcacttc gcgtactttg ctcctgtctt
     4141 tatacgctcc agcgataacc actacgcggt cacctttttt gattttgagc ttcggagcga
     4201 agcgtttttg ctgattttta gcagccatga tattgatatt aaaacagctt cataaatgag
     4261 ccttcagcac tattaaagta cctcgggagc caaagaaacg atacgcatat agtcacgttc
     4321 gcggagttcg cgggccacgg ggccgaagat gcgggtgccg cgaggctcgc cctgagcatt
     4381 gagcaacacc actgcgttgt cgtcaaaacg aatataggta ccgtccttac gacgaatttc
     4441 ctttttggtg cgcacaacga cagccttaga aacagtacct tttttgatac cgcccggact
     4501 ggcgtctttc accgaaacga cgatctgatc gccgatggag gcataacgcc ggtgtgaacc
     4561 acccagtaca cggatgcaca acacctcttt tgccccgcta ttgtcggcta ctctgagcct
     4621 ggattcctgc tggatcatga cacaaaatta ttttgctctt tcaatgattt ctaccaaacg
     4681 ccagcgtttg aatttgctca acgggcgtgt ttccatgatt ttgactacat ctccgatgtt
     4741 gcattcgcct ttctcgtcgt gagccaaaaa tttattggat ttcttcacga atttaccata
     4801 gatagggtgc tgaagcctcc gttctacaga aacggcgatg gtcttgtcca ttttattgct
     4861 ggtgactacg ccgactttcg tctttcttaa atttctctcc gtcatggtat aatgatttgc
     4921 gataccccgc tttgaattat ttgcctttgc ttctgcgtgt acgaatcttg gagcggctct
     4981 gcaggccggc ttcgtccatt gcggctactt cccgacggcg gatttccgtt tggactcggg
     5041 cgatgtcgcg gcgcatttcc ctgatgttca gaggattatc caatcctttg agtgcatgat
     5101 caaaacgcag tttttgatat tggacttcaa gctgcgccag ctctccttcg aggtcggcgt
     5161 cggtgaattc ctgtagttct aaaaatttat tacttgccat ttcaaaaata ttgaaagatc
     5221 acacagatga tttactcctg ataatcggga cgggtaatgg ttttggtcat caccggcagc
     5281 ttctgagcag ccaggcgcaa tgcctcagca gctactgcat aaggtacgcc atccaactca
     5341 aacatgatgc gccccggctt aatctgagct acccaatggt cgagagagcc tttgccctta
     5401 cccatacgca cttcctgcgg cttggcagtg atcggcttat ccgggaaaat gcgaatccaa
     5461 actttccctt cccttttcat atagcgggtc atggcaatac gcgcagcttc tatctggcgg
     5521 ttggtcaaac gacctccggt aacggctttg agaccaacag acccgaaggc aatagtgctc
     5581 ccccggtaat ccaagccttc atttctgccc ttctgctgct tgcgatattt tgttcttttt
     5641 ggctgtaaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtgataa atcctaacgt cttttttgat
     5761 ttcttcttcc gccgccgcct tcgcctctgt cgcctctttc tcctccacga ccacgaccgc
     5821 cgtcacgacg atctccacgg cctccatctc tgcgttcgcc gcgttccggg cgaccgccca
     5881 ttccacgctc cttgccgcca ggcgtcgttt ccttcttggt cacgccgata ttggggctaa
     5941 ggtcgcgttt gccgaacact tcacctttgc aaagccagac tttgatgccg attttgccat
     6001 acacggtttg tgcttcgccc aatgcgtagt ctatatctgc gcggaaggta tgcaaaggcg
     6061 tccgaccgtc tttgaactct tcggtacgag acatttccgc gccnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnt gcgaactttg attccctcgg caccggcgcg catcgtagcc tgaatagcag
     6181 tcttgatggc acgacggtag ttgatacggg cttcgagctg cttggcaacg gattcggcaa
     6241 caatggctgc atccatttcc ggcttgcgaa tctcaacgat attgatttga gactctttac
     6301 cggtgagttt tttcagttct tcgcgaatac gatcaacctc ttgccccccc ttaccgatga
     6361 tgatacccgg acgggaagta tggatggtaa ccgtgatgcg cttgagagtg cgctcgatga
     6421 tgatacgagc aatgcctcct ttggcaatac gagcattgag gtaggtgcga attttttcat
     6481 cttctaccac tttctgcccg aagtctttac ctccgaacca gttggaatcc cagccccgga
     6541 tgataccgag ccgattgccg attggatttg tcttctgacc cataattgag tattaaaaaa
     6601 cttaagcgtt gtcttaattt tcagtttctg cttcctgagc cgtcactacc tcgctgtcca
     6661 attgcttgcg gttttccacc acaatggtag catggcaggt acgcttgcga actcggtggg
     6721 cgcgaccgtg cggcgcaggt tggaaacgct tgaggaatgt gccttcatcc acaagagccg
     6781 ttttgatata aagctcataa tcatcggcat tttccatatt gtccaacttg tattcccagt
     6841 tggcaatggc agagaggagc actttctcca accaggaacc ggactcccgc ttggtatagc
     6901 gaaggatgcc caaggcctga cttacgtttt tgccacgaat attgtccatt accaagcgca
     6961 tcttgcgagc cgacatcgga cagtttttca atttggctac tgcttccatg ttgcttcttt
     7021 tttcgaagat ggagatgatt acccgttttg gtatgaaccg ccgggcaagc gcatcaatac
     7081 tttatttttt acgattaccg gagtgtcctc tgaagttgcg ggtaggagcg aattcgccca
     7141 gcttgtggcc taccatgttt tcagtaacgt acaccggtac aaatgttttg ccgttatgaa
     7201 ccgcgatggt ttctcctacc atgtcgggga taatcatcga ggcgcgagac caggttttga
     7261 tcaccgcttt tttgttggac tgcttggcct tgaatacttt atccaacaac ttgtggaata
     7321 tgtacggccc ttttttaatt gatcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnncgttt actttttatt agacgacttg cgacgggaaa taatcaactt cgttgactgc
     7441 ttcttgttgt tgcgagtctt caagcccttg gccggcttgc cgttgcgaga acgtgggtga
     7501 ccaccggaag cccgaccttc gccaccaccc attgggtgat ctaccgggtt cattgcaaca
     7561 ccgcgtacac gcgggcgacg tcccaaccaa cgactgcgac cagctttgcc cgatacgatc
     7621 aagctgtggt cggggttgga agcaactccg atggtagctc tgcaagtaag caaaaccctg
     7681 cggaattcgc cggaaggcag gcgcaaagat gcgtacttgc cttcacgtcc cgtaagcact
     7741 gcgtaagtac ccgcactacg agcgcctttg cccggctgca actcgatagc gtggatttca
     7801 gcgcccagcg gaatttcact cagcaacatc gtattgccga tagtgggagt aacgccagag
     7861 ccggaaagca acactgcgcc cactttaaga ccgtgaggag cgatgatgta gcgcttctct
     7921 ccgtcttcgt attgcagcag agcgatgaat gccgagcggt tggggtcata ctcaatagag
     7981 accaccgttg caggtactcc g
//
LOCUS       DAORLF010000206         7995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4894_length_7995_cov_1.927960, whole genome shotgun sequence.
ACCESSION   DAORLF010000206 DAORLF010000000
VERSION     DAORLF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4894_length_7995_cov_1.927960"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..>645)
                     /locus_tag="PK198_15660"
     CDS             complement(105..>645)
                     /locus_tag="PK198_15660"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRF40228.1"
                     /translation="LHDCRVGARCILHANAVVGSDGFGFAPQPDGTYKKIAQIGNVVI
                     EDDVEIGAGTTIDRASLGSTILRKGVKLDNLIQVAHNVEIGENTVIAAQVGIAGSTKI
                     GRNCRIAGQVGFIGHVTIADNTSIQAQSGVAQNVTESGTIICGYPAFAYRDYIRSHAV
                     FKRLPELDKKIKQLEDKLK"
     assembly_gap    646..870
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<871..>1094)
                     /locus_tag="PK198_15665"
     CDS             complement(<871..>1094)
                     /locus_tag="PK198_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HRF40229.1"
                     /translation="PDVLVNRPGKIEEGQAGEITFLGNDKYEPFIYTTQASVVLVNKD
                     FSPRQAIQATLIRVENVYAALAFLLDKFGA"
     assembly_gap    1095..1217
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1448..1530
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1566..>1773
                     /locus_tag="PK198_15670"
     CDS             1566..>1773
                     /locus_tag="PK198_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF40230.1"
                     /translation="MNIIDWIVMLGALLGIAAYGIYRTRHVSSVKSYLLGDRQLPWWT
                     IGLSIMATQASAITFLSTPGQAYED"
     assembly_gap    1774..1793
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1794..3290
                     /locus_tag="PK198_15675"
     CDS             <1794..3290
                     /locus_tag="PK198_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF40231.1"
                     /translation="MRFIQFYYGLPIAMVILSVFVIPLYYRLNVYTPYEYLEKRFDLK
                     TRTLTAVLFLVQRGLAAGLTIYAPSIVLSSILKWDLNATILFIGIIVIVYTMSGGTMA
                     VSVTQKQQMIVILLGLFVAAGVIIYKLPREVGFGGAVDLAGHLGKLNSVTFDLNFKDR
                     YNIWSGMIAGVFLFLSYFGTDHSQVSRYLSGKSLTESRLGLMFNGLLKVPMQFLVLFV
                     GILVFVFFQFNRPPVHYIDSNLAMLAGTPHEAKYREIERQYKDVFQQKEAAMTRIISA
                     ARATDQPTLDAARSEAEMLQKKDATLRKFAKIEVMRASPTAKTKDTDYVFLSFVMEYM
                     PVGVVGLLLAMIFAAGMSSTSSELNALTSVMAIDVYKRYWAKDKSDGHYLLASKLFTL
                     MWGLLALLFAATAQLFENLIQMVNILGSLFYGTILGVFLTAFFMPKIRGNAVFIAALI
                     TQANIIFLFVLERGGKLPISFLWYNPIGCLLLMLLAWMIQMAFGKRDK"
     gene            complement(3304..3687)
                     /locus_tag="PK198_15680"
     CDS             complement(3304..3687)
                     /locus_tag="PK198_15680"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRF40232.1"
                     /translation="MNALLQFLQFGIVGVGGMAIDFGATWLLKEKLRLNPYIANSIGF
                     SLAVVNNFFWNKYWTFHDNSEAVEIQFAKFLTISIIGLLLNNGAVYLLHKRFGMRFYI
                     AKLFATGLVLLWNFGANYRFTFHSE"
     assembly_gap    3717..3744
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3745..>4225)
                     /locus_tag="PK198_15685"
     CDS             complement(3745..>4225)
                     /locus_tag="PK198_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRF40233.1"
                     /translation="GPHIAYKIVPEDSLSIIGSQSKNAPGELYTIEVRFDIAQDLHRR
                     NKRLLDLALAGLLLFSLPVHLLFVKNRTGLITNILQVLFAQKTWVGYASGADSGHTLP
                     ALRPGVLSPLDALPQGSLRPDDAATIQRLNMLYAKNYRAENDLEIVLKGYRRLGVRQ"
     assembly_gap    4226..4240
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4241..5728)
                     /locus_tag="PK198_15690"
     CDS             complement(<4241..5728)
                     /locus_tag="PK198_15690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF40234.1"
                     /translation="MKLTVVIVNYNVRHFLEQALLSVRKAIARIDAEVWVVDNNSADD
                     SVAMVQTKFPEVRLIANTDNTGFAVANNQAIRQSTGEYVLLLNPDTVVEEDTFEKCVQ
                     FMDAQPEAGGLGVRMIDGSGKFLPESKRGFPSPWTAFCKTFGLSRIFPRSKVFNHYHL
                     GYLDEHQTHEVEVLAGAFMLLRRSALDKAGLLDEAFFMYGEDIDLSYRILLAGYKNYY
                     FPETSIIHYKGESTKKGSLNYVRVFYQAMIIFARKHFTGRRAALFVLMLQAAIWFRAA
                     LTLLSNLARRAYLPLLDSVLIFSGLALLKNFWASYYHHDAGYYDSSFLWVNAPLYTVL
                     WLAGVYFSGGYEEQGNLRRLMRGLVVGTLFIAAVYGFLDMAYRSSRALILLGAAWSAT
                     GMIALRTALHLARYGNLNVGRESQRQVVIVGSLEESRRVLRLLQQAQAPVHCIGMVSP
                     HNHFEPADFLGSVSKLQDIALIYRINEIIFCAADVPSQEIMRQMTK"
     gene            complement(5825..6661)
                     /locus_tag="PK198_15695"
     CDS             complement(5825..6661)
                     /locus_tag="PK198_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF40235.1"
                     /translation="MMKRFLGLLPALFFALVVNAQDGWEAGPWLGISYYFGDLNTNYN
                     LSRPGPAGGFAARYNFNDRICLRFGVAGGQISADDALSGNTYERARNLNFQSTLVDGS
                     AQLEFNFLPYIHGHRDYFFTPYLLAGINVTNFNPKTEYEGQLVELRPLGTEGQLRGEE
                     YYTTSLGWMYGGGFKIDLSYRLSLNVEIAGRALATDYLDDVSTVYADKSDLRRRGDLA
                     VALSDRSITLPGVDGSQLGRPGVQRGNSKNNDTYFFIGAGILYYFGDLKCPDSGSRKR
                     RR"
     gene            6827..>7995
                     /locus_tag="PK198_15700"
     CDS             6827..>7995
                     /locus_tag="PK198_15700"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRF40236.1"
                     /translation="MNRTALIFLSLLLAALFLIPSEAQAQRRRRTPAKRPVSAMVTDS
                     KLFNQAHSGFILFDPQTQKVLQQRNADKYFTPASNTKIFTLRTALHFLSDSLPVLRYL
                     DMGDTLVFWGTGNPLFLHPDLPGDSSVLAFLRSRPETLFFTDFNFRDDHFGPGWSWAD
                     YSDYYQPERSPMPIYGNIARFSSRGADSGFVVSPALWRDSLFFGPQLEEERGAVIRRT
                     ERGNRFEFNSEARRRTGFSDDIPFDYSPQLMVKLLSDTLRRTVRLFDAAMLPAGSVNT
                     LYMPADDALYARLMQQSDNFIAEQLLMMCSEQLFSIQNSRKMIDHAKDICFKDVPDRM
                     EWWDGSGLSRYNFFTPRTVVHVLNQLYQQQPQERLFRIFPAGGVSGTIRGWYAGP"
BASE COUNT         1691 a         2089 c         2008 g         1713 t
ORIGIN      
        1 gtcagaggaa agaaaaatga caaaccgtcc ggacagcaga tgatttttat caccatcagg
       61 tttgtgaggg aagatggcag aggattgaaa aaagaatata gaaactactt cagtttgtcc
      121 tccaactgtt tgatcttttt atccaattcc ggcaagcgtt tgaacacggc atgagagcga
      181 atataatcgc ggtaagcgaa ggccggatag ccgcaaataa tggtgcccga ttccgtcacg
      241 ttttgcgcca cgcccgactg agcctgtatg ctcgtgttgt cggcaatggt gacatgcccg
      301 atgaagccca cttgcccggc aatgcggcag ttgcgtccta ttttggtgct gccggctatg
      361 cccacctgtg cggcaatgac ggtgttttcg ccaatttcca cattgtgcgc cacctgaatc
      421 aggttgtcca atttgacgcc tttgcgaagg atggtagaac ccagcgacgc gcgatctatg
      481 gtagtgccgg cgccgatttc cacatcgtct tctatgacga cgttgccgat ctgggctatt
      541 tttttgtaag ttccgtcagg ctgtggggca aatccgaacc cgtcgctgcc caccactgca
      601 ttggcatgca aaatgcagcg ggcgcccacc cggcaatcgt gtaaannnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcgccgaa cttgtccaac aggaaggcca
      901 gcgcggcata cacgttttct accctgatga gcgtggcctg tatggcctgc cggggcgaga
      961 agtccttgtt gaccaacacg accgaagcct gtgtggtgta gatgaaaggc tcgtatttgt
     1021 cgttgcccaa aaaggtgatc tcaccggctt ggccttcttc aattttcccg gggcggttca
     1081 caaggacatc cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnaaa gcgtatgcca cattccaacc cgcattctga tgaaacaccg
     1261 ccgcccatat ttcgcacctg gtcaggtgtt tatgtcttcg ttctcggcgc aagttttgtc
     1321 gtttttctgc ttctgctttg gctcagcctt tcctatcagt aaatcagtag gcggtgtgcg
     1381 gcaggcggtg tgcagtgcta agcgtgagga aacatggaac cttctcctgt ttagtcaagg
     1441 ggtgactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacacaaaaa ctttgaacac gaaacacaaa
     1561 atatcatgaa catcattgac tggatcgtta tgctgggcgc tttgctgggt attgccgctt
     1621 acggcatcta tcgcacccgc catgtgagca gcgtcaaaag ctacctcctc ggcgaccgcc
     1681 aactgccctg gtggaccatc ggcctgtcca ttatggccac gcaggccagc gccattacct
     1741 ttctgtccac gcccggccag gcctacgagg acgnnnnnnn nnnnnnnnnn nnnatgcgct
     1801 tcatccagtt ttactacggc cttcccattg cgatggttat tttgtcggtg ttcgtcattc
     1861 cgttgtatta ccggctcaat gtttacacgc cctacgaata tctcgaaaag cgtttcgacc
     1921 tcaaaacccg aaccctcacg gctgttctgt ttttggtgca gcgcggcctg gcggcgggtc
     1981 ttaccatcta tgcgccatcc atcgtgttgt ccagcatttt gaaatgggac ctcaacgcca
     2041 ccattttgtt catcggcatc atcgtcattg tttacaccat gtcgggcggt acgatggcgg
     2101 tgagcgttac ccaaaaacag cagatgatcg tcattttgct gggcttgttt gtggcggcgg
     2161 gcgtcatcat ctacaagctc ccgcgcgagg tgggcttcgg cggcgcggtg gatctggcgg
     2221 gacatttggg caagctcaat tccgtcacat tcgacctcaa tttcaaagac cgctacaaca
     2281 tctggtcggg tatgatagcc ggggtgtttc tgtttctgtc gtacttcggc accgaccact
     2341 cgcaggtatc gcgctacctc tccggcaagt cgctcaccga gagccgttta ggtctcatgt
     2401 tcaacggcct gctcaaggtg cccatgcagt ttttggtact gttcgtgggc attttggtgt
     2461 ttgtattttt tcagttcaac cgcccgccgg tacattatat tgactccaat ctggccatgc
     2521 tcgccggtac gcctcacgag gccaaatatc gcgaaataga gcgccaatac aaggatgtgt
     2581 tccaacaaaa ggaagcagcc atgactcgca tcatttcagc agcaagggct accgaccaac
     2641 ccacattgga cgcagcccgg tcggaggcgg aaatgctcca gaaaaaggat gctacactgc
     2701 gcaaattcgc aaaaattgag gtcatgcgcg cctcccccac cgccaaaaca aaagacaccg
     2761 actacgtttt cctctccttc gtcatggagt acatgccggt gggcgtggtg ggtctgttgc
     2821 tggccatgat cttcgcggcg ggcatgtcgt ccacttcttc cgaactgaac gccctcacca
     2881 gcgtcatggc catagatgta tataaacgct actgggcaaa agacaaaagc gatggacact
     2941 acctcctggc ttccaagctg ttcacgctta tgtgggggct gttggcacta ctgtttgccg
     3001 ctaccgccca gcttttcgag aatctcatcc aaatggtcaa tatcctcggc tcgctgttct
     3061 acggcaccat tttgggcgtg ttcctcacgg catttttcat gcccaaaatc aggggcaatg
     3121 ccgtttttat agcagccctc atcacgcagg ccaatatcat tttccttttt gtactcgaac
     3181 gcgggggcaa gctccccatt tccttccttt ggtacaatcc catcggctgc ctgctactca
     3241 tgctgttggc ctggatgata caaatggctt tcggcaaacg ggataaatga tttttgcggc
     3301 tttttattca gagtggaaag tgaaccggta attggcgccg aaattccaca gcagtaccag
     3361 cccggtagcg aacaatttgg cgatgtaaaa gcgcattccg aaacgcttgt gcagcagata
     3421 aaccgcgccg ttgttgagca gcagcccgat tatggagatg gtgagaaatt tggcaaactg
     3481 tatttctacg gcctcgctgt tgtcgtggaa agtccagtat ttattccaaa agaagttgtt
     3541 gaccaccgcc agagaaaaac cgatggaatt ggctatgtag ggattcagtc gcagcttttc
     3601 tttgagcagc caggtagcgc cgaagtctat ggccatgccg ccgacgccta cgatgccgaa
     3661 ttgaaggaat tggagaaggg cgttcatacg gcagcaaaat tactgacttt tccccgnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnttattg cctcaccccc agccgccgat accctttcaa
     3781 tacaatttcc aaatcatttt ctgcgcggta attcttggca tacaacatgt tgagccgttg
     3841 gatggtagcc gcgtcgtcgg ggcgcaaact gccttggggc agggcatcca aggggctgag
     3901 tacgccgggg cgcagggcag gcaaggtgtg gccgctgtcg gcgccggagg catatcccac
     3961 ccaggttttt tgggcaaaaa ggacttgtaa aatattggta ataaggcctg tgcggttctt
     4021 tacaaaaagc aggtgaacgg gcaggctgaa caacaacaac cccgccaggg cgagatcgag
     4081 caggcgtttg ttgcggcggt gcaagtcctg cgcaatgtcg aagcgcacct ctatggtgta
     4141 aagctcgccg ggggcgtttt tcgactggct gccgatgatg ctgaggctgt cttcgggcac
     4201 aattttataa gcgatatgag gaccgnnnnn nnnnnnnnnn tttcgtcatc tgtcgcatga
     4261 tttcctgaga cggcacatcg gcggcgcaga agatgatttc gttgatgcgg taaatgagcg
     4321 caatgtcttg caatttggac acagacccca gaaaatcagc gggttcaaaa tgattgtgcg
     4381 gcgataccat cccgatgcaa tgcacgggag cctgcgcctg ctggagcaat cggagtacgc
     4441 gccggctttc ctccagactg cccacaatca ccacctgccg ctgcgattcc ctgcccacgt
     4501 tcaggttgcc gtatcgggcc aaatgcaagg ctgttctcag cgcgatcatg ccggttgccg
     4561 accaggccgc ccccaacaga atgagcgccc gcgaggagcg gtaggccatg tccaaaaaac
     4621 catatacggc agcaatgaac agcgtaccga ccaccaatcc gcgcatgagg cggcgcagat
     4681 tgccctgctc ctcgtagccg ccgctgaaat acaccccggc cagccagaga acggtgtaaa
     4741 gcggagcatt gacccacaaa aaactactgt cgtaatagcc ggcatcgtgg tggtaatacg
     4801 aggcccagaa gtttttcaac aaagctaatc cactgaaaat caacactgag tccaacagcg
     4861 gcaagtaggc gcgccgggcc agattggaca acagcgtgag cgccgcccga aaccatatag
     4921 ccgcctgaag catgagcaca aacaaggccg cccgccgacc ggtgaagtgc ttgcgggcaa
     4981 aaataatcat ggcctggtaa aaaacgcgga cgtagttgag actgcctttt ttggtgctct
     5041 cgcctttgta gtgaatgatg gacgtttcgg gaaagtagta gtttttatac cccgccagca
     5101 ggatgcgata cgagaggtct atgtcttcgc cgtacataaa aaacgcctcg tcgagcaggc
     5161 cggctttgtc caaagcgctg cgccgcaaga gcatgaaagc gcccgccagc acttctacct
     5221 cgtgggtctg gtgttcgtcc aaatagccga ggtggtaatg attgaagact ttggaacgag
     5281 ggaaaatgcg cgacaggccg aaggttttgc aaaaggccgt ccagggcgag gggaagccgc
     5341 gcttcgattc cggcagaaac ttgcccgaac cgtctatcat gcgcacgccc aggccgcctg
     5401 cttcgggctg agcatccatg aactgcacgc atttttcaaa ggtatcttct tccaccaccg
     5461 tatcggggtt gagcaggagg acatactcgc ccgtactttg ccggatggcc tgattattgg
     5521 ctacggcaaa gcccgtgttg tcggtattgg caatgaggcg tacttccggg aatttagttt
     5581 gtaccatcgc cacagagtcg tcggcggagt tgttgtccac cacccaaacc tcggcgtcta
     5641 tgcgggctat ggccttgcgt accgagagca gcgcctgctc taaaaaatgg cgcacattgt
     5701 aattgacgat aacaacagtg agcttcatga atcaatgtgc tgatgtggcc tcgcggcctg
     5761 aactcgtagc tcgcagctca tggctcgcag cttttggatg accactatca ttaagcatcc
     5821 tgcatcacct gcgccgtttg cggctgccgg aatccgggca tttcaaatct ccaaaatagt
     5881 agagaatacc cgcgccgatg aagaaataag tatcgttgtt tttgctgttg ccgcgttgta
     5941 cgccgggccg ccccaactga ctaccgtcca cgcctggcaa cgtgatggaa cggtcggaga
     6001 gcgcaacggc caagtcaccc cggcggcgga gatcgctttt gtcggcatac acggtgctga
     6061 catcatccag atagtcggtg gccaatgccc ggcctgcaat ttccacattg aggctgaggc
     6121 ggtagctgag gtctattttg aagcccccgc catacatcca gcctaagctg gtggtgtaat
     6181 actcctcgcc ccgcaactgc ccctcggtgc ctaagggacg caactccacc aattggcctt
     6241 cgtattcggt tttaggattg aaattagtca cattgatacc ggccagcagg tagggcgtga
     6301 aaaagtagtc gcgatgaccg tgaatgtagg gcaggaaatt gaactccaac tgagcgctgc
     6361 cgtccaccag cgttgattga aaattgagat tgcgggcgcg ctcgtaggta ttgcccgaaa
     6421 gggcgtcgtc ggcgctgatc tgaccgccgg caacgccaaa gcgcagacag atgcggtcgt
     6481 tgaaattgta acgggccgca aaaccgccgg cagggccagg gcggctgagg ttgtaattgg
     6541 tattcagatc gccgaaatag taggaaatgc ccaaccaggg gccggcctcc catccatcct
     6601 gcgcattcac aaccaaagca aaaaacagag cgggcagcag gcccaaaaat cgcttcatca
     6661 ttcagtatat attgatattg agggcgcaaa ggtaggaagg caaacggaga atacagcgaa
     6721 tatgccgccg gcttcctgct aaaaacaccg ccgggataac ggaaaaagcg cggcctctgt
     6781 tatgccaccg ggaaatttgt agctttcttt gccgacaaaa aaacgcatga atcgcactgc
     6841 tctcatcttt ctgtcgctgc tgttggccgc cctgttcctc attccgtcag aagcccaggc
     6901 acaacgccgc cgccgcaccc ccgccaagcg cccggtgagc gccatggtga ccgattccaa
     6961 gctgttcaat caggcgcatt ccggctttat tttgttcgac cctcaaacgc agaaagtact
     7021 ccagcaacgc aatgccgaca agtatttcac gccggcttcc aacaccaaaa tcttcacgct
     7081 gcgaaccgcc ctgcactttc tgagcgacag cctgccggta ttgcgctatt tggacatggg
     7141 cgatacgctg gtgttttggg gcacgggcaa cccactgttt ttgcaccccg atttgcccgg
     7201 cgacagcagc gtgctggcat ttttgcgctc ccgaccggaa actttgttct tcaccgattt
     7261 caacttccgc gacgaccact tcgggccggg ctggtcctgg gccgattaca gcgactatta
     7321 ccaacccgaa cgctcgccca tgcccatcta cggcaatatt gcgcggttca gttctcgcgg
     7381 cgccgacagc gggtttgtgg tcagcccggc actgtggcgc gacagcctct ttttcgggcc
     7441 gcaattggag gaagagcgcg gcgccgtcat tcgcagaacg gagcgcggca accgctttga
     7501 gttcaactca gaagcccggc gccgcacagg tttttccgac gacattccct tcgattactc
     7561 gccgcaactg atggtcaaac tgctctccga cacgctgcgg cgcaccgtgc ggctcttcga
     7621 cgcggctatg ctgcccgcag gttcggtgaa taccctgtac atgcccgccg acgacgcgct
     7681 gtatgcccgc ctgatgcaac aaagcgacaa tttcatcgcg gaacaattgc tgatgatgtg
     7741 ctccgagcag ttgttcagca tccaaaacag ccgcaaaatg atagaccacg ccaaagatat
     7801 ctgtttcaaa gacgtgcccg accgcatgga gtggtgggat ggttcgggct tgtcgcggta
     7861 caattttttc acaccgcgca cggtggtaca tgtattaaac cagttgtacc aacagcagcc
     7921 gcaggagcgc ctgtttcgca tttttcctgc cggcggcgtg tcgggcacca tccggggctg
     7981 gtatgccggc cccaa
//
LOCUS       DAORLF010000207         7929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4950_length_7929_cov_1.967742, whole genome shotgun sequence.
ACCESSION   DAORLF010000207 DAORLF010000000
VERSION     DAORLF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4950_length_7929_cov_1.967742"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /gene="miaE"
                     /locus_tag="PK198_15705"
     CDS             <1..335
                     /gene="miaE"
                     /locus_tag="PK198_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HRF40237.1"
                     /translation="RKDEYVNELLKFQKKGGSREEALLEKLLVCALIEARSCERFRLL
                     SLYINDKALRGFYHKFMISEAGHYRLFIDLAKQYAGEEKVKIRWQEYLTREAEIMEEL
                     ELRGDRMH"
     gene            complement(562..1125)
                     /locus_tag="PK198_15710"
     CDS             complement(562..1125)
                     /locus_tag="PK198_15710"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF40238.1"
                     /translation="MDSYNWEKTGSENGPHIKLFQVRYDWMCNPRNGAEERMVILEGR
                     DSANVVALTSDNQMLLIRQFRFGIGADTLELPGGLMEAGEELQDAAARELIEETGYSS
                     ENWEYLGKVPSNPVFMDSYIHHWVARDVQYSQAPALDDGEAVALVFLPIEKVLDMLLA
                     GEFEHPHTVTALLRFFVGTGLLISGRK"
     assembly_gap    1151..1211
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1212..>1510
                     /locus_tag="PK198_15715"
     CDS             <1212..>1510
                     /locus_tag="PK198_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40239.1"
                     /translation="VLTANLSAQCSAGNGTQFSDCLTSGSTTINISGTFTVNGNVDLS
                     GKTINLGSGNKELTLTGIVTISSTTVFSGSGNVKVINGGNSACGNSGQCIINVATL"
     assembly_gap    1511..1555
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1556..2113
                     /locus_tag="PK198_15720"
     CDS             <1556..2113
                     /locus_tag="PK198_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40240.1"
                     /translation="LSGILPVELTAFKALPGTSGIDLLWSTASELNNARFLIERSGSD
                     GYFRSIGEAKGAGTTSTPQDYQFTDRAPLPGSNYYRLRQEDYDGKFEYSQVIAVQSRE
                     SSAEAMKVAPNPVDGRQIRLEMPSAEMPVRIELMDAAGKRTSLVFSAMERAMEADLPQ
                     NLSAGMYYLTAVFADRVLVSPIVVR"
     gene            complement(2116..>2693)
                     /locus_tag="PK198_15725"
     CDS             complement(2116..>2693)
                     /locus_tag="PK198_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40241.1"
                     /translation="DDVLPVVFTKTRTDSILAACLLFRKRIDEQLNSKPDAAVATILK
                     RADLALQGYELRAERLHSDPSFYDIGVYLNTVLVLNDPIDKRMEYLALQLQRTQAYYA
                     AARANLRPTEAESIYNAIDVQRSTLLFLQTQLPDSIQIASLQPNEKTALTKAVHQSRL
                     EVKDYVAFCRSLLYELQNQGIEQELEKKLRK"
     assembly_gap    2694..2703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2822..>3054)
                     /locus_tag="PK198_15730"
     CDS             complement(2822..>3054)
                     /locus_tag="PK198_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRF40242.1"
                     /translation="ERVRRRQLELTAKGLELTEESVRENLLERDRIDSTRADSPLRQA
                     DDAIVIDNTLLNRQEQLELALQLAHETMKQPA"
     assembly_gap    3055..3064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3065..3510)
                     /gene="cmk"
                     /locus_tag="PK198_15735"
     CDS             complement(<3065..3510)
                     /gene="cmk"
                     /locus_tag="PK198_15735"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRF40243.1"
                     /translation="MRNIIIAIDGYSSCGKSTLARDLASALRFGYVDSGAMYRAVTLY
                     FLNHSIDYDDRAAVIEALRHIRIEFRNINHQNRTFLNGQDVEDDIRDMRVSSAVSPVA
                     AIPEVRRALVQQQKALGQTGGIVMDGRDIGTVVFPDAELKIFLTADP"
     gene            complement(3560..5080)
                     /locus_tag="PK198_15740"
     CDS             complement(3560..5080)
                     /locus_tag="PK198_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40244.1"
                     /translation="MKELLEITQILDKKKISKIEILDQNSLRQPNNLFARMYNGLYKE
                     KFETDEQAAQELYQATPTDARYRQLKSRFRKRLLNTFFFLDPSLAQLSGRSAALYSCR
                     RNLALVHILLINGATETALGEAQQIQNMAIRFGFPDMVVASALIIREIYAGQNDEKNY
                     SKQDKFIETYEPARNAEHLSEKYIQFIRLAYQSSEALHASTLSEISTRCDELLQLSEQ
                     HTAPLINFNAFEAWMLRFELEGDFASAADVAEQAEDYLKKQATHYDDDKLKLFAIGRL
                     RAALHDKDFKKGRSFAENAFRLIRPHDPERLRLMEFYLLLSIHTESILHAFAVYREAI
                     EHKQFHHAPETEQDKWRIFTPYMHLLMELRPLQQQFVISQKRKDFKVEEFVESPVPYL
                     PQKENQTILIAVAQIMFFFHVRNLTAAGRQIVWLHKLAQNLAKKGIYPRQCAFIGLLH
                     ELHKNEYRPPSGKRCDKFLEAFNRHPFRYRGQLQDLEIIPYEKLWEMTLGLVTGQK"
     gene            complement(5265..5486)
                     /locus_tag="PK198_15745"
     CDS             complement(5265..5486)
                     /locus_tag="PK198_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRF40245.1"
                     /translation="MYWTLDLAYHLEDAPWTATRDELIDYAIRSGAPVEVIENLQEME
                     DEDEIYEGIEDIWPDYPRKEDFLFNDDEY"
     gene            complement(5610..6794)
                     /locus_tag="PK198_15750"
     CDS             complement(5610..6794)
                     /locus_tag="PK198_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40246.1"
                     /translation="MKRVASLLLLFWIFLNRVFCMAGEEMQSVASVPFGMGTLEVQYS
                     LRMKQATPTRPDETGIMDAYHHLLESDYAVLLSDLEDKKDSLRLNDWMFYTLVRSSVD
                     AIFEKQRAFDRELMVWFLMSHAGFDTRLGYNEEKAFLYLRSEEEVFGLPMIESRRQSY
                     YHIPFPLRYPVEAERVYLLAFDAMPGGRSLSFRWEEAPVLKPAFAEKKLDIRTGDTLY
                     QLSVQYDLAWVLTLAHQPVLSEADYFNMPVSRTLSASLLPQLRRITEGKSTRKALEII
                     AAFTRSSFDYQDDMKAYGRSRPMTPDEVFHYRYSDCEDRAVLFAYLVKEVLQLPVIVI
                     AFQDHITIGVGGDWDSSAAVTYLGRRYGICDPTGPVQSTELGVWPQGYENQSYVVIAA
                     RL"
     gene            complement(6816..>7203)
                     /locus_tag="PK198_15755"
     CDS             complement(6816..>7203)
                     /locus_tag="PK198_15755"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRF40247.1"
                     /translation="QALPAGFVKGVDFLTVQLKAFGQMFKGKIKASDSLGGFASIGQM
                     FGSEWLWERFWSMTAALSIILAFMNLLPIPALDGGYVMFLLFEMVTGRKPSDKFMEWA
                     GTIGFFILIALMLWANGLDVLRGCSR"
     assembly_gap    7204..7221
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7222..>7929)
                     /locus_tag="PK198_15760"
     CDS             complement(<7222..>7929)
                     /locus_tag="PK198_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF40248.1"
                     /translation="ESNDTAFLDQPPQPWEFRTKPAWQRLIIMLGGVIVNFLLGGLIY
                     AGVLAYWGEEFLPAKNVEYGIYADSLGQKIGLQDGDKILMVGEQPFVKFSEPLVKRAI
                     IIENVDRLTVERAGQNVVINVPADFTKELAAHENKDKVLFGVRLPFIAGELHKGSPAE
                     KVGIKVGDQIIGYNDIATPYFQDFQRLASKNPNKDVSVKVLRNKTDTLSFALKLTEEG
                     KAGIYPKGPGEFFELQR"
BASE COUNT         1886 a         2001 c         2013 g         1885 t
ORIGIN      
        1 agcgcaagga cgaatacgtg aacgagttgc tgaaatttca gaaaaaaggc ggcagccgcg
       61 aggaggcgct gttggaaaaa ctgctggttt gcgcactgat tgaggcgcgg agttgcgaac
      121 gcttccgctt gctttctctg tacatcaacg ataaggcgct gaggggattc taccacaagt
      181 ttatgatatc cgaggcgggg cattatcgcc tttttattga cctggccaag caatacgcgg
      241 gcgaggaaaa ggtaaaaatc cgttggcagg agtacctcac ccgggaagcg gaaatcatgg
      301 aggaactgga gttgcgagga gatcggatgc attgaagcgg tatgttcggt aggcggtggc
      361 cgcgcctgtg gcgcggggtg ctaagcgcgg ctccaacccg aaacattgaa cccgaaacaa
      421 gacccgttcc taccggaacg cccaaatttt taacgcgaat cttagcgaag ggcgcgaaat
      481 ttcgcgaacc acgcctccgt cgtggcgcag cgcgagagga acgcgaaacc cgaaaccttg
      541 aacccgaaac ccgaaacaaa cctatttcct acccgaaatc aaaagccctg ttcctacaaa
      601 aaacctcagc agcgccgtca ctgtgtgggg gtgttcgaac tcgccggcca gcagcatatc
      661 caatactttt tctatgggca agaacaccaa tgccaccgct tctccgtcat ctaaagcagg
      721 agcttggctg tattgcacat cgcgcgctac ccagtgatga atgtagctgt ccatgaacac
      781 cggattggaa ggcaccttgc ccagatattc ccagttttct gatgagtatc ccgtttcctc
      841 tatcagttca cgggcggcgg catcttgcag ctcctcccct gcttccatca gaccgccggg
      901 cagttccagt gtatcggcgc cgatgccgaa gcgaaactgg cgaatgagca gcatctggtt
      961 gtcggaagtc agagccacaa cattggcaga atcgcggcct tcgaggatga ccatgcgctc
     1021 ctcggcgccg ttgcggggat tgcacatcca atcataacgc acctgaaaga gcttgatatg
     1081 cggcccgttt tcagagcctg ttttttccca attgtaggag tccataagca ttgtgttact
     1141 ttttaaagcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn ngttttaacg gcaaatttat cagcgcagtg cagtgcaggc aatggtactc
     1261 aattctcaga ttgcctgact tccggcagca cgacgatcaa cataagcggc acctttacag
     1321 taaacggaaa cgttgacttg tccggcaaga ccatcaatct ggggagtgga aacaaagaac
     1381 tcacccttac aggaatcgta acgattagca gcactaccgt tttttcaggc agcggcaatg
     1441 tgaaagtcat caatgggggc aactctgcgt gcggcaactc cggtcagtgt atcatcaacg
     1501 tggctacgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgag
     1561 cggtattctc cccgtagaac tgactgcttt caaagccttg cccggaacct ccggcattga
     1621 cctgctttgg tccaccgctt ccgaactcaa caatgcccgc ttcctcattg agcgcagcgg
     1681 cagcgatggg tacttccgca gcatcggcga ggcaaaaggc gcaggcacta cctctactcc
     1741 tcaagattac caatttaccg accgcgctcc gttgcccggc tccaactact atcgcctccg
     1801 gcaggaggat tacgacggca agtttgagta ctctcaagtc attgccgtac aaagccggga
     1861 gtcaagtgct gaagccatga aggttgcccc caatccggtt gatggccgtc aaatcagatt
     1921 ggaaatgccc tcggctgaaa tgcccgtccg catagaactc atggatgcag ccgggaagcg
     1981 tacctcactc gttttcagcg caatggagcg agctatggag gccgacttgc cgcaaaacct
     2041 ctccgccggc atgtattatc tgaccgctgt gtttgccgac agggtattgg tgtcccccat
     2101 tgtggtgcgg taaaattatt tccgcaactt tttttccaac tcctgctcga tgccctgatt
     2161 ttgcagctcg tacaataggc ttcggcaaaa agcgacataa tccttcacct ccaaacggct
     2221 ctgatgaacg gctttcgtca gggccgtttt ttcgttgggt tgcaggctcg ctatctggat
     2281 ggaatccggc agttgggttt gtaaaaaaag cagggtactg cgctgcacat caatggcatt
     2341 gtagatggat tccgcctctg tggggcgaag gttggcacgg gcggcagcgt agtaggcttg
     2401 ggtgcgttgc aactgaagcg ccaggtattc catccgctta tcaatagggt cgttgagtac
     2461 cagcacggta ttgagataga cgccgatgtc gtaaaaagaa ggatcggagt gcaggcgctc
     2521 ggcgcgcagt tcgtatccct ggagggcaag atcggctctt tttagaatcg ttgccaccgc
     2581 agcatcgggc ttggagttca gttgctcgtc tattcgtttt ctaaacaaca ggcaagccgc
     2641 gagaatagaa tctgtgcggg tctttgtgaa aaccaccggc aacacgtcat ctgnnnnnnn
     2701 nnnaaaagga gggggatttg ttccagcatt tgccgttcca atttccgcgc tttgccttcc
     2761 ggcgaactgc atcccgacga caccagcaag agtacgcagc ttatcatcca tccatatttc
     2821 atcatgccgg ctgtttcatg gtttcgtggg ccaattgcaa agccagttcc aactgttcct
     2881 ggcgattcag caaagtatta tctatcacaa tggcgtcgtc ggcctgcctg agcgggctgt
     2941 cggcgcgggt ggaatcaatg cggtcgcgtt ccaacaggtt ttcccgaaca ctctcctccg
     3001 taagttccaa tcccttggcg gtcagttcta attgtctgcg ccgtacgcgc tcgtnnnnnn
     3061 nnnngggtcg gcggtgagga agattttaag ttcggcatca ggaaaaacca ccgtaccgat
     3121 gtctcggcca tccatgacga tgccgccggt ttggcccagc gctttttgtt gctgcaccaa
     3181 ggcgcgccgt acttcgggaa tggcagccac ggggctgacg gctgaggaaa cgcgcatatc
     3241 gcggatgtcg tcttccacat cctggccatt gaggaaagtg cggttttggt ggttgatgtt
     3301 tctaaactca atgcgaatgt gacgcaaggc ttcgataacg gctgcgcggt cgtcgtagtc
     3361 aatgctatga ttgagaaaat aaagcgtaac ggcccggtac atggcgccgg aatctacata
     3421 tccgaaccga agtgccgaag ccaagtcgcg ggccagggtg cttttaccgc aggaagagta
     3481 gccgtctatg gcaatgatga tgtttctcac gaggtgttgg ttgatgaact cagcggcaaa
     3541 aataggtctt atttgacatt tatttttgcc ccgttaccaa tccgagcgtc atttcccaga
     3601 gcttttcgta aggaatgatt tcgaggtctt gcaactgccc tctgtatctg aacgggtgcc
     3661 ggttaaatgc ttccagaaat ttatcacacc gtttgccgga aggcggtcgg tattcgtttt
     3721 tatgcagctc gtgcaaaagg ccgatgaagg cgcattggcg gggatagatg ccttttttag
     3781 ccagattttg ggccaatttg tgaagccaca ctatctgtcg cccggcggct gtgagattgc
     3841 gcacatgaaa gaaaaacatg atctgtgcca cggcaataag aatggtctgg ttctcctttt
     3901 gcggcagata cggcaccggc gattccacaa actcttccac cttgaagtct ttccgtttct
     3961 gcgaaatcac aaactgttgc tgaagcggcc tcaattccat caacaggtgc atgtagggcg
     4021 taaaaatacg ccatttatcc tgctctgttt ccggcgcgtg atgaaactgc ttgtgttcta
     4081 tcgcctcccg atacacggca aatgcatgca aaatgctctc ggtatgaatg ctgagcaaca
     4141 gataaaactc catgagccgg aggcgttcgg ggtcgtgagg acgtatgagc cggaaggcgt
     4201 tttcggcaaa agaccgtcct tttttaaagt ccttatcgtg cagggctgcc cgcaatctgc
     4261 cgatggcaaa tagtttgagt ttgtcgtcat cgtaatgcgt agcctgcttt ttcaggtagt
     4321 cttcggcctg ctcggccaca tcggcggcag atgcaaaatc accctccaac tcgaatcgaa
     4381 gcatccatgc ctcgaaagcg ttaaaattga tcagcggagc agtatgttgc tcagagagtt
     4441 gcagcaattc atcgcatcgg gtactgattt ccgacagtgt ggacgcatgc agcgcttcgc
     4501 ttgattggta ggccaagcgg atgaactgta tgtacttttc agacagatgc tcggcattcc
     4561 gcgcaggctc ataagtttct ataaatttgt cctgtttgga ataatttttt tcatcgtttt
     4621 gtccggcata aatctcccgg atgatgagcg ccgaagcgac gaccatgtcc gggaagccga
     4681 agcggatggc catgttttgg atttgctgcg cttcgccgag agcggtttct gtggccccgt
     4741 tgatgagcaa aatgtgtact aaagccaaat tgcgacggca ggaatagagc gcggcgctgc
     4801 gtccggagag ttgcgccaga ctgggatcga gaaaaaagaa ggtattgagc agcctttttc
     4861 tgaatctgga tttgagctgc cggtagcggg cgtcggtcgg agtggcctga tacaattctt
     4921 gtgcggcctg ctcgtcggtc tcaaattttt ctttgtacaa accattgtac atgcgcgcaa
     4981 acaggttgtt gggctgccta aggctgtttt gatccagaat ctcaattttg ctgatcttct
     5041 ttttgtctaa aatctgagtg atttcgagca gctctttcat gtttgcttat ttaattggca
     5101 ggatagtgaa ggagtgacta acccaggaaa gtcactcgta gtcgcccctt cactacgcat
     5161 tgaaatgata ccggcaaaaa aaatggtttt acagcaggaa actgtaaaac catgattctt
     5221 tttttaatgg cggcaaaagc cccgctgcgg agtaccgctg aaaatcagta ctcgtcgtcg
     5281 ttgaagagga agtcctcttt gcgcggatag tcgggccaga tgtcttcaat gccctcgtaa
     5341 atttcgtcct cgtcttccat ttcctgaagg ttttcaataa cctcaacagg ggcgcccgaa
     5401 cggatggcat agtcaataag ttcatcgcgc gttgcggtcc agggagcatc ttccaagtga
     5461 tatgccaggt ccaatgtcca gtacatggta attaagattt tacagttact tctgatagat
     5521 gttgtgaaaa agtgtgcgaa taacgaaatt ccaaagcaaa cgttacaaaa cttgtctgca
     5581 tttttttaaa aaaaattcaa tgtacctcat cagaggcggg cggcaatcac cacataagac
     5641 tgattttcat agccttgtgg ccaaacgcct aattcggttg attgtaccgg gccggtgggg
     5701 tcgcatattc catatcggcg gccaaggtaa gtgacggcgg cgcttgagtc ccagtccccc
     5761 cctaccccga tggtaatgtg atcctgaaaa gcaatgacga tgacaggcag ttgcaagacc
     5821 tctttcacca gataagcaaa caataccgcg cgatcttcgc aatctgagta tcggtaatga
     5881 aacacctcgt cgggggtcat cgggcggctc ctgccatacg ctttcatatc atcttgataa
     5941 tcaaatgaag accgcgtaaa tgccgctatg atttccaatg cctttcgagt agatttgccc
     6001 tcggtgatgc gcctgagctg cggcaaaagg gaagcactca aagtccttga aaccggcata
     6061 ttgaaataat cggcctccga caataccggt tgatgagcca gcgtgagcac ccaggcgaga
     6121 tcgtactgaa cggatagttg atacagcgtg tcgccggttc tgatgtctaa cttcttttcg
     6181 gcaaaagcag gttttaaaac gggggcctcc tcccagcgaa acgacaatga cctgccgccg
     6241 ggcatagcgt cgaaagcaag caggtaaacg cgctccgcct ctaccggata acgaagcgga
     6301 aaaggaatgt gatagtacga ctgccggcga ctctcgatca tcggcaaacc gaaaacctcc
     6361 tcttcggaac gcaaatacaa aaaggctttt tcctcattgt agcccaaacg ggtatcaaaa
     6421 ccggcatgag acatcagaaa ccagaccatc agctcccggt caaacgctct ttgtttttca
     6481 aaaatggcgt ccacactgga gcgcacaagt gtataaaaca tccaatcatt gaggcgaagg
     6541 ctgtcttttt tgtcttccaa atcgctcagc aatacggcat aatcgctttc caataaatgg
     6601 tggtaagcat ccatgattcc ggtttcatcg ggccgggtag gtgtagcctg cttcatgcgc
     6661 aaactgtatt ggacctccaa tgtgcccatg ccgaacggaa ccgacgcaac cgattgcatc
     6721 tcctcgccgg ccatgcagaa gacacggttc agaaaaatcc agaacagcag caacaaggaa
     6781 gctactcttt tcataatgac ttaccaatga gttgtttatc tcgaacagcc gcgcagcaca
     6841 tccaatccgt tggcccacaa catcaatgca atgaggatga aaaatccgat ggtaccggcc
     6901 cattccatga acttgtcgct gggcttccga ccggtaacca tttcaaacag cagaaacatg
     6961 acataaccgc cgtcaagagc tggaatgggc agcaggttca taaacgccag aatgatggac
     7021 aaggctgccg tcatgctcca gaagcgctcc cacagccatt cagaaccgaa catctggccg
     7081 atggaagcga aaccgcccaa gctgtcgctg gctttgatct tgcctttgaa catctgcccg
     7141 aatgccttga gttgcaccgt gaggaaatca acgcctttca caaaaccggc gggcagggcc
     7201 tgcnnnnnnn nnnnnnnnnn ntttctttgc agctcgaaaa actcgccggg gcctttggga
     7261 taaatgccgg ccttgccctc ctcggtgagt ttcagggcaa aagaaagcgt gtctgttttg
     7321 ttgcgcaata ctttgacgct cacgtctttg ttcggatttt tggacgccag ccgctggaaa
     7381 tcctgaaaat agggcgtagc aatgtcgttg tatccaatga tctgatcgcc gactttgatg
     7441 ccgacttttt cagcaggtga gcctttgtgc aactcgccgg ctataaaagg caaacgaacc
     7501 ccaaacaaaa ccttgtcttt attctcatgg gcggccaatt ccttagtgaa atcagccggc
     7561 acgttgataa ccacattctg accggctctt tccaccgtca gccgatctac gttttcaatg
     7621 atgatggcgc gtttgacgag cggttcgcta aattttacga acggctgctc gcctaccatc
     7681 agtattttat cgccgtcctg aagacctatt ttctgcccca gagaatccgc gtaaatgcca
     7741 tattctacat tcttggcggg cagaaactcc tcgccccaat aggccagcac tccggcataa
     7801 atgaggccgc cgagcaggaa gttgacgatg acgccgccga gcatgatgat gaggcgttgc
     7861 caggccggtt tggtgcgaaa ttcccagggt tgcggcggct gatcgaggaa ggccgtgtcg
     7921 ttgctttcg
//
LOCUS       DAORLF010000208         7911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_4963_length_7911_cov_1.904277, whole genome shotgun sequence.
ACCESSION   DAORLF010000208 DAORLF010000000
VERSION     DAORLF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_7911_cov_1.904277"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK198_15765"
     CDS             complement(<1..218)
                     /locus_tag="PK198_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HRF40249.1"
                     /translation="MRILLIEDELPAARQLARLLRDARPDAAILETLDSVEGAVSWLR
                     TFPPPDLIFMDIQIADGLSFDIFQQVEVA"
     assembly_gap    249..378
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<379..1278)
                     /locus_tag="PK198_15770"
     CDS             complement(<379..1278)
                     /locus_tag="PK198_15770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF40250.1"
                     /translation="MKTTSTPAQCIGFDDRLLRIIGIPLSAALIPLVFFKNLPYDWYT
                     ILNTLLYTAFIWEGVRGIFIWATRRFPEFRQWRTRLLWIIALCVVYVGSACTVVGIIT
                     ELFLPKSLQLRANPEYLESYFASYFMLLAVSGIYESMRFFTLWKTALLEKEQAEQARL
                     AGQLEGLRNQVNPHFLFNSLNTLTYLIPEEPERAVRFVQQLSKVYRYMLESRDAQLIA
                     LQEELNFLDAYVFLMRERFGDNLLVSLRTGELPPDTAIVPLTMQMLFENAIKHNVISA
                     QKPLHIEVFAEQGGLIVRNNLQRK"
     gene            complement(1308..>1660)
                     /locus_tag="PK198_15775"
     CDS             complement(1308..>1660)
                     /locus_tag="PK198_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40251.1"
                     /translation="SDNGRALVALALEESAYGQVWHLPVGEPITPEEATEIFNRVLGT
                     QFKSGFMPDILLGFLSLFIKVLKEVAEMQYQNKTPYLMSFEKFRRQFPDFETTPYETG
                     FREMAASFQTGQSV"
     assembly_gap    1661..1683
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1684..2258)
                     /locus_tag="PK198_15780"
     CDS             complement(<1684..2258)
                     /locus_tag="PK198_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF40252.1"
                     /translation="MSSELHVVLGASGASGRAVVQELKRRNLPFRAIGRSAHFSDFEM
                     TAADVFNPDELLRAVQGASHVYLCIGLPYSTKIWQQQWPLLMQNTIEVCETVRARLIF
                     LDNIYMYGPAPLAVPFDETHAQHPTSRKGIVRKQTADMLLQAIEDKRVDALIGRSADF
                     YGAQAVNSGLYVSFLDRMLKGKSPLSIAPKGV"
     gene            complement(2363..>3056)
                     /locus_tag="PK198_15785"
     CDS             complement(2363..>3056)
                     /locus_tag="PK198_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40253.1"
                     /translation="GYDFTPAPDWRFKAEAYYQWISKAPVDPYSSSFSLLNAGADFVF
                     PDRGSLVNSGTGTNIGIEFTAEKFFSKGYYGLFTLSLFDSQYKGSDGIARSTAFNGRY
                     VVNLLAGKEFPLDKAGRRVLTLDTKVTRAGGRPYTPVDLEASRRDGVQRDVESQAFSL
                     RLDDYFRWDFKIGFRSNSSKRKMSQTFFLDFQNMTNNDNVFAMRYNPVRGEVGRTNQI
                     GFFPDILYRLEF"
     assembly_gap    3057..3223
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3224..4767)
                     /locus_tag="PK198_15790"
     CDS             complement(<3224..4767)
                     /locus_tag="PK198_15790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40254.1"
                     /translation="MKNVQLLFVLLLLLGAQAMWAQTQTIRGIVADRQSEMPLIGATV
                     ELLNSAGVQGAVTDVDGSFALTGVPVGRRELRVSYLGYEPATLTNVLVTAGKEVILDI
                     RLEESLVSLSEVVVKAGNDKDKAVNDLATISARQFNTEEVMRFSGGRNDVGRLVANFA
                     GVAASNDARNDIIIRGNSPTGVLWRLEGIPIPNPNHFSTLGTTGGPVSALNPNLIANS
                     DFLTGAFPAEYGNALAGVFDINLRRGNRERAEYMFQLGAFSGLEALLEGPLNKSKGGS
                     YLISYRHSFVELADVVGLSFGTTALPRYKDLSFNFDLGQSKAGRFSVFGIGGDSKIDF
                     LGKDVDSTDLFANPSQNAYNTSRFGVAGIKHQILLNDRAYLRTVLSVSHNGTTFQSED
                     LELTDDNGPLPLTDVNDRLNTLRLSSFYNDKINARLSLRTGILLQQQYLKTRVDSREG
                     QPDRDGDGLRDWVNQRNFDGGFFQGEAFVQTQYRMGEALTLNTGLRALYFSKTEDLAV
                     EPRIAF"
     gene            complement(5084..>5271)
                     /locus_tag="PK198_15795"
     CDS             complement(5084..>5271)
                     /locus_tag="PK198_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF40255.1"
                     /translation="TVSPKRGWPLLLFAVALTVGVSRMYLVQHFWKDVYFGALIGVVL
                     AWGVYVRSGRARYGCAG"
     assembly_gap    5272..5343
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5344..5712)
                     /locus_tag="PK198_15800"
     CDS             complement(<5344..5712)
                     /locus_tag="PK198_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40256.1"
                     /translation="MSFIRENRLFFLCFAVFAVGGAVLLEYLPQGEEIIWLSRHRSPA
                     ADVFFKGATLLGEGYIFFAVGFVFLYLRRRDWIWSSVLGLLVMLTAYGAKTWFAHDRP
                     YAWFQKMGQWAAVTPVPGVDM"
     gene            complement(5709..>6678)
                     /locus_tag="PK198_15805"
     CDS             complement(5709..>6678)
                     /locus_tag="PK198_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF40257.1"
                     /translation="KVRTRVFDLSEFDLQRTNEDLFKSNRSMLSVRQLQDAIDSMAVQ
                     ELDRQKASTEQVAAYIAPPAPDTSAVAQDSAAQSYQPRPPVRDTAGPQLWADTSMQAG
                     VKKEAKGAIKIAADTDSLVNVPVQIQDLPQQTFTGSLANLDPNAPQGIRQEITQPLNE
                     YESLVKTFIAYEQPRLVQSALTQARSLMGQAEANVSILSRMKKNRVKFIYDLHMKYVF
                     AAMCVVFLFIGAPMGAIVRKGGFGFPILIATIFFVLFIILTIFCRKIAEAFIVPGNLA
                     AWLPCLLFIPLGMYLTVKAMNDSEVFRFEAIGHLWRRLRPAKKEAA"
     assembly_gap    6679..6688
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6689..7394)
                     /locus_tag="PK198_15810"
     CDS             complement(<6689..7394)
                     /locus_tag="PK198_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF40258.1"
                     /translation="MKIIDRLLVQSFLPPFVVTFMIATFVLLIQILWVYIDDLTGKGL
                     GFLLIAELLAYKCVSLVPMALPLAILISSVMVLGNLAEHYELSSLKSAGVSLFRVMGP
                     MAAFGVVCAGVSYYCSDYLIPVSNLKFGSRMYDIQRQKPALRLDEGVFNDDFQGFAIR
                     IGNKKGDGQGIEDVLIYDHKSSDEGSYNQINARSGKMFTSSDQKYFVMKLRDGHQYSE
                     PRAGSRGSAASPFVRTN"
BASE COUNT         1779 a         2104 c         2012 g         1614 t
ORIGIN      
        1 gctacttcca cctgctggaa aatgtcgaaa ctgaggccgt cggcaatctg gatgtccata
       61 aaaataaggt cgggcggcgg aaaggtgcgc agccagctca cggcgccctc tacgctgtcc
      121 aaggtttcga ggatggcggc atcgggccgg gcatcgcgca ggagccgggc gagctgccgg
      181 gcggcgggca attcgtcttc aatgagcagg atgcgcatga tgggaaggat ggagtgaaac
      241 caaaggcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnntt tgcgttggag gttgttgcgc acaatgaggc cgccctgctc
      421 tgcaaacacc tcgatgtgga gcggtttttg ggccgaaatg acgttgtgtt tgatggcatt
      481 ttcaaacaac atctgcatcg tcaggggcac aatggccgta tcgggcggca gctcgccggt
      541 gcgcagactc accagcagat tgtcgccgaa acgctcgcgc atcaggaaga cgtaggcgtc
      601 gaggaagttc agttcttcct gtaaagcaat gagttgcgcg tcgcggcttt ccaacatgta
      661 gcgatacact ttgctgagtt gttgtacaaa acgcacggcg cgctccggtt cttcgggaat
      721 cagatatgtg agggtattga ggctgttgaa caaaaaatgc gggttcacct gattgcgcag
      781 tccctccaat tgacctgcca aacgcgcttg ctcggcttgt tctttttcca aaagagcggt
      841 tttccaaagc gtgaaaaacc gcatgctttc gtaaataccg ctcacggcca gcaacatgaa
      901 ataggaggca aaataggact cgaggtattc cgggttggca cgcaattgaa gggatttcgg
      961 cagaaacaat tccgtgataa tccccaccac ggtgcaagcc gaaccgacat acacgacgca
     1021 cagcgcgatg atccacaaca ggcgggtgcg ccactgccga aattcgggaa agcgccgggt
     1081 agcccatata aatatgcccc gaacgccctc ccaaatgaag gccgtataaa gcagcgtatt
     1141 gagtatggtg taccagtcgt aaggcaggtt tttgaaaaaa accagcggaa tgagcgccgc
     1201 cgacaagggg atgccgatga tccgcagcag gcggtcgtcg aaaccgatac attgcgcagg
     1261 cgtgctggtc gttttcatgg cagaaaggta ggtagtttat ttcaaaacta tacactttgc
     1321 ccggtttgaa acgaagctgc catttcccga aaacctgttt cgtagggcgt cgtttcaaag
     1381 tctggaaatt gccggcgaaa tttctcaaaa ctcatcaaat agggcgtctt gttttggtat
     1441 tgcatctctg caacctcctt taataccttg ataaacaagg acaaaaaacc aagcaatatg
     1501 tcaggcataa aaccggattt gaactgcgtg cccagcaccc tattgaaaat ttccgttgct
     1561 tcttccggcg taatcggctc gcccaccggg aggtgccata cctgcccgta agcactctct
     1621 tccaatgcaa gcgctaccaa tgccctgccg ttgtcggagg nnnnnnnnnn nnnnnnnnnn
     1681 nnnaccccct ttggcgcaat agacagcgga gatttgcctt tgagcatacg gtctaaaaag
     1741 gaaacataca aaccgctgtt caccgcttgg gcgccgtaaa agtctgccga gcgcccgata
     1801 agcgcgtcca ctcttttgtc ttctatggct tgcagcaaca tgtccgccgt ttgcttccga
     1861 acaattcctt tccggctggt gggatgttgt gcatgagttt catcaaaagg cacagccagg
     1921 ggcgccggac catacatgta aatgttgtcc aaaaagatga gtctggcgcg cacggtttcg
     1981 cacacttcta tggtgttctg catgagcagc ggccactgtt gttgccatat ttttgtgcta
     2041 tatggcagcc cgatgcagag gtaaacgtgg cttgcgcctt gcaccgccct cagcaattcg
     2101 tctgggttaa aaacgtcggc ggcagtcatt tcaaagtcgc tgaaatgggc gctgcgtcca
     2161 atcgcccgga aggggaggtt gcgccttttc aattcctgta cgacagccct gcccgatgca
     2221 cccgatgcgc ctaatacaac atgcaattct gaactcatct ccttatgttt ttgtgctgaa
     2281 aagatataat ttgcgttaag ctgcaagctg ctaaacactc agtttgcagc ttgcagctta
     2341 cggcttgtag ccaacagttt ccttaaaatt cgagtctata cagtatatcc gggaaaaacc
     2401 cgatctgatt ggtacgtcct acttctccgc gcaccgggtt gtaacgcatg gcgaacacat
     2461 tgtcgttgtt ggtcatgttc tgaaaatcga ggaagaaggt ctgcgacatc ttgcgtttgc
     2521 tgctgttgct gcggaaaccg attttgaaat cccagcggaa gtaatcgtcc aaacgcaagc
     2581 tgaatgcctg cgattctaca tcgcgctgaa caccgtcgcg gcgactggcc tccaggtcca
     2641 ccggcgtgta aggccgcccg ccggcgcggg tcactttggt atcgagggtg agtacccgcc
     2701 gaccggcttt gtctaaggga aactccttgc ccgccagcaa attgaccaca tagcgcccgt
     2761 tgaatgcggt gctgcgtgct atgccgtcgc tgcccttgta ttgcgagtcg aacagcgaga
     2821 gggtgaacag gccgtagtac cctttgctga agaatttctc agccgtgaat tcgatgccga
     2881 tgttggtacc ggttccgctg ttgacgagcg agccgcgatc ggggaataca aaatctgcgc
     2941 cggcattgag caaagaaaaa ctgctcgaat acgggtccac gggcgctttg ctgatccatt
     3001 ggtaatacgc ctctgctttg aaacgccagt cgggcgcggg cgtaaaatcg tagcccnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgaagg caattcgagg
     3241 ctcgacggcc agatcttcgg ttttggaaaa atacagcgcc cgcaggccgg tattgagcgt
     3301 gagggcttcg cccatgcgat attgggtttg cacaaaagcc tcgccctgaa aaaagccgcc
     3361 gtcgaaattg cgttggttta cccagtctcg gaggccgtcg ccgtctcggt cgggctgccc
     3421 ttcgcggctg tccacgcggg ttttgaggta ttgctgctgg agcaggatgc cggtgcgcag
     3481 actcaggcgg gcattgattt tatcgttgta aaaagaagac aggcgcaggg tgttcaagcg
     3541 gtcgttcacg tcggtgagtg ggagcgggcc gttgtcgtcg gtcagttcta agtcttccga
     3601 ttggaaagtg gtgccgttgt gcgaaacgct gagcaccgtg cgcaggtagg cccggtcgtt
     3661 gagcaggatt tggtgtttga tacccgccac gccgaagcgg gaagtgttgt aggcgttttg
     3721 gctcggattg gcaaacagat cggtagagtc cacgtctttg ccgaggaagt caatcttgct
     3781 gtcgccgccg atgccgaaca cggagaacct gccggctttg ctttgtccca ggtcgaagtt
     3841 gaaactcagg tctttgtacc tcggcagggc agtagtgccg aagctaagcc ccactacatc
     3901 agccagttct acaaaagaat ggcggtagga aatgaggtaa gaaccgcctt tagatttgtt
     3961 gagcgggcct tcgagcaagg cttccagccc cgaaaaagcg cccaattgaa acatgtactc
     4021 ggcgcgctcg cggttgcccc gccgcaggtt gatgtcgaac acgccggcca gcgcgttgcc
     4081 gtattcggcc ggaaaagcgc cggtaaggaa atcgctgttg gcaatcaggt tgggattgag
     4141 cgcagatacc ggtccgcccg tggtgccgag tgtgctgaaa tggttgggat tgggaatcgg
     4201 aatgccctcc aaacgccaca agacgccggt gggagaattg ccccggatga tgatgtcgtt
     4261 gcgggcgtcg ttgctggccg ccacgcctgc aaaattggca accaagcggc ccacgtcatt
     4321 gcgcccgccg gaaaagcgca ttacttcctc tgtgttgaac tgccgggcag aaatagtagc
     4381 caaatcattg acggccttgt ctttatcatt gccggctttg accaccacct cgctgagcga
     4441 caccaaagat tcctccaaac ggatatcgag gatgacctct ttgccggcag ttaccagcac
     4501 gttggtcagc gtggcgggtt catagccgag gtaagacacg cgcagttcgc ggcgacccac
     4561 cggtacgccc gtcaaggcaa aagagccgtc cacatcggta acggcgcctt gcacgccggc
     4621 gctgttcagc aattccaccg tagcgccgat gaggggcatt tcgctttggc ggtcggccac
     4681 tatgcctcga atggtttgtg tttgcgccca catagcctgc gcaccgagga gcagcagcag
     4741 gacgaagagc agttgtacat ttttcatgtt tcgatgtttt tttgtacaaa cttgcagttc
     4801 cgacgcccgc ttttgcaact gaaaaacggt gagtggaaaa ataagcgggg tgaatggcag
     4861 tggaggcggg ttgagcagca ctcccggtta cgcccgcctc tgaaaaaccg gacatgaaag
     4921 ccttatttgg aatttggaga gacgacaata aatcattgga cgaattgcgc aaagctgcct
     4981 ggggagaccg gttatgaaag tacttattta tatggtaaat tgagtttctg tgcttagtga
     5041 aggggtgact acctgtgtaa atccttgctg agtcacccct tcactaccct gcacaaccat
     5101 atctcgcacg cccactcctc acatacaccc cccaggccag caccaccccg atcagcgccc
     5161 cgaaatacac gtccttccag aaatgctgca ccaaatacat gcgcgaaaca cccaccgtca
     5221 gcgccaccgc aaacaacagc agcggccacc cgcgcttggg cgacaccgta annnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnncatgtcc acacccggca ccggcgtcac ggcagcccac tgccccattt tctgaaacca
     5401 ggcatagggc cggtcgtgtg caaaccaggt tttggccccg taggccgtca gcatcaccag
     5461 caagcccagc acagaactcc atatccagtc gcggcgacgc aggtataaaa acacaaatcc
     5521 caccgcgaaa aaaatgtagc cctcgcccag cagcgtagcg cccttaaaaa acacatcggc
     5581 tgccggcgag cgatgccggc tcagccagat gatctcctcg ccttgcggca aatactccag
     5641 caaaacagct cctcccaccg caaaaacagc gaagcacaaa aaaaacagcc ggttctcgcg
     5701 aatgaaactc atgccgcctc ctttttggca ggccgcaatc ggcgccacag atggccaatc
     5761 gcctcaaaac gaaatacttc cgagtcgttc atggccttta ccgtaaggta catacccagc
     5821 ggaatgaaca gcaggcaggg cagccaggca gccagattgc cgggcacgat aaatgcctcc
     5881 gcaatcttgc ggcaaaagat ggtgaggatg atgaacaaca caaagaaaat ggtggcaatg
     5941 agaatcggaa atccgaagcc gcccttgcgc acaatggcgc ccataggcgc gcctataaac
     6001 aaaaacacca cgcacatggc cgcaaaaacg tacttcatgt gcaggtcgta aatgaatttc
     6061 acccggttct tcttcatccg gctcaagatg gacacattgg cctcggcctg ccccatgagg
     6121 ctgcgcgcct gcgtcagggc cgactgcacc aagcgcggct gctcgtaggc aatgaaggtt
     6181 tttaccaacg attcgtactc gttaagcggc tgagtgattt cctgccggat gccctgcggc
     6241 gcgttggggt ccagattggc caaactccct gtaaaggttt gctgcggcaa gtcctgtatt
     6301 tgcacgggca cattcaccag cgagtcggta tcggccgcta ttttgatggc gcctttggcc
     6361 tcctttttta cgccggcctg catcgaagta tccgcccaca attgcggccc ggcagtatcc
     6421 ctcaccggcg ggcggggctg gtacgattgc gccgccgaat cctgcgccac agcgctcgta
     6481 tcgggcgcgg gcggcgctat ataggccgcc acctgctccg tagatgcctt ctgccggtcc
     6541 agctcctgca cggccatcga atctatggca tcctgcaatt gccgcacgct cagcatactg
     6601 cggttgcttt taaacaagtc ctcattggtg cgttgcaggt caaattccga caggtcgaac
     6661 acgcgggtcc tcaccttgnn nnnnnnnnag ttggtgcgca caaaaggcga cgccgcactg
     6721 ccccggctgc ccgcgcgcgg ctccgaatat tggtgcccgt cgcgcagttt catcacaaaa
     6781 tacttctggt cggaactggt aaacatcttg ccgctgcgcg cattgatctg attatacgag
     6841 ccttcatccg acgatttatg gtcataaatg agcacatcct ctatgccctg cccatcgccc
     6901 tttttattgc cgatgcgaat cgcaaaaccc tgaaaatcat cattaaacac cccctcatcc
     6961 aagcgcaggg ccggtttttg tcgctgtata tcgtacatcc gagaaccgaa tttcaggttg
     7021 gaaaccggga tcagataatc ggagcaataa taagacacgc ccgcacaaac caccccgaaa
     7081 gccgccatcg gccccatgac gcgaaacagc gacacccccg ccgatttcag gctcgacaac
     7141 tcgtagtgct ccgccagatt gcccagcacc atcaccgacg aaataaggat ggccaaaggc
     7201 agcgccatcg gcaccaacga cacacacttg tacgccagca attccgcaat gagcaaaaag
     7261 cccagcccct tgcccgtcag gtcgtctata tacacccaca gaatttgtat gagcaacaca
     7321 aacgtggcaa tcataaacgt gacgacaaac ggcggcaaaa aactctgtac cagcaggcga
     7381 tcaataatct tcatggcagt tattcccttg cccgcaacaa acagcgtgca ggcagcgatg
     7441 caaaggtaat gcttattttc ttttggggcg ccccggccac tctgaagtac ccaatggccg
     7501 tcgggtcatc cgctccatgc agcatcgggc cactctgccc tatgccctat gctgcatccg
     7561 ctgctgcccc tggcgcggcg gggcaatgac acaaagcgcg atgcggtacc gacagctttt
     7621 gcctccgaaa aagccccgca ccaatggcgt atatatccga ttacaagacc tgctgggcca
     7681 cgaaagcagt aagacaacgg agatatatac gcactacttg tcccggcgcc accgggatca
     7741 cgcgaatcgg gtctgaccgg gtgcagagtc cttttgacag gctccatctg tgaaaaagtt
     7801 ttttcacgca acagttgctt ttatatcaaa cttccattag ctttgcgcag tcaaatccag
     7861 aatggactcc tgtgaaaaaa ctgaacgcag gaagtttcta ttgaggttgg a
//
LOCUS       DAORLF010000209         7853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5017_length_7853_cov_1.846371, whole genome shotgun sequence.
ACCESSION   DAORLF010000209 DAORLF010000000
VERSION     DAORLF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5017_length_7853_cov_1.846371"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>220)
                     /locus_tag="PK198_15815"
     CDS             complement(<1..>220)
                     /locus_tag="PK198_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40259.1"
                     /translation="IINISSVNAQRAQFGQVNYAAAKAGMHGFSKTLAIEVANKGITV
                     NTISPGYIGTDMVMAVAQEDPDKIVQTIP"
     assembly_gap    221..399
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<400..612)
                     /locus_tag="PK198_15820"
     CDS             complement(<400..612)
                     /locus_tag="PK198_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40260.1"
                     /translation="MTKDGKYIALVTGAVGGLGTDMCKQLADDGFFVIANYRTQNKAD
                     IWQQEMKAQGYDFAMAKADVANYDEVG"
     assembly_gap    784..836
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <837..2233
                     /locus_tag="PK198_15825"
     CDS             <837..2233
                     /locus_tag="PK198_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF40261.1"
                     /translation="DYAVGSIQFSPIVVGLSLAASITSAATFIINPGFIALYGFSGIL
                     ALAIVLPIGLFFSLVILTKSFRKYGQSVKALTMAQWIGTRYDSKGFALFFAFLSLLLL
                     TFIVLICVGMTKVMSNALNVKEIYVLLGLVVFVFGYMMFGGANSMVYTNTIQAVLMLI
                     VAFILLGSGFEHFKGGVNGFIDKLAAIDPALVSLTNPGSPLFRDYFEIIFCNLIVGIA
                     IVCQPHILTKSLLLKKESDVNRYLVTGIIVEMIFFAVVIAGLYARLSFPDLTTNGQKI
                     NMDGIIPAYVVAEFPVYVGILVIMGLLSAGLSTLEGLIQSLSTTITNDIIAPVGGIEK
                     TSGKLVWINKVVIIALAVTAVLLTNHQLQHPNLSVGIFAQNGVYAFFSAAFMPVLLGV
                     FTKNTPRIAPIAAAITAVVVHFSVYYIGITPYMQGAVRNPAIAATLAILSSAVVGTAL
                     YFLLRKPETAPSQV"
     gene            2245..3105
                     /locus_tag="PK198_15830"
     CDS             2245..3105
                     /locus_tag="PK198_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF40262.1"
                     /translation="MLDWVYPYPLKMAHLPEQVSVAYIDEGAGPQTLVFLHGLGSYLK
                     GWTKNVSELSRDFRCIALDFPGYGKSSKGDLPYSMTFFARVVRDFIQRLNLQNVVLAG
                     HSMGGQVAATVAVRYPEIVQKLVLIAPAGFETFTETDRVWIRAAYSPTLLKAMPTDQI
                     RRNFEVNFSRFPDDAGFMIDDRMMMLGTQAYEQYCEMIPRCVNSMLDEPVFDLLPQIK
                     APTLVLFGENDVLIPNKLLHPALNPVMVAETGARRIPAARLELLRPCGHFAQWECAGA
                     VNRAVRRFCG"
     gene            complement(3335..3625)
                     /locus_tag="PK198_15835"
     CDS             complement(3335..3625)
                     /locus_tag="PK198_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF40263.1"
                     /translation="MKTHFDEAFLEASTEILRAIAHPIRIAIVDLLHTHQRLSVTEIF
                     KKLKIEQAIASHHLRILKNQDVVDVQRDGKNSYYALCKPEYYEIVQTLTKVV"
     gene            3738..3902
                     /locus_tag="PK198_15840"
     CDS             3738..3902
                     /locus_tag="PK198_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40264.1"
                     /translation="MENTIPVRIYDFLKGHPPFHLFDKNTLLDLAAQAIVQYRPKGEL
                     VFNRELCPAD"
     gene            3887..>4895
                     /locus_tag="PK198_15845"
     CDS             3887..>4895
                     /locus_tag="PK198_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF40265.1"
                     /translation="MPGGLIFVLKEGAIHLFHEEKGERELVDQCDEGELFGLRPLLAD
                     DAYALTAVAAEETLLYAIPVESFREAAEQAPGVLMYIASNFASGWQRRTTPESKGKIF
                     AGQNNSGQEAFQLVEIQSIERSKSPVVCRPEQPIQEAAGIMTEKEVGSIIVVNEHRHP
                     IGIVTDKDLRKKAATGRVPISAPVSEIMSQPVITAHPSITVADVQMRMVKHKIPHLCL
                     TENGNNDAPVVGVLTEHDLLVVQGNNPAVFLREIKRAQTTSELLHIRERAEQLLEKYI
                     RQEVAIAYIASIITEINDALIQRVIRLAEQRMDAEGRQRPTARYCWLALGSEGREEQL
                     LR"
     assembly_gap    4896..4915
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4916..>5324
                     /locus_tag="PK198_15850"
     CDS             <4916..>5324
                     /locus_tag="PK198_15850"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRF40266.1"
                     /translation="VFEDVPEQAHEATKSYYLQLSGYVTTMLNECGFEYCPAEMMASN
                     PNWCLSLSEWKEQFSKWMLEPDPQSVMMCTIFFDYRPVYGLSELPEALTAHIFSIIDQ
                     EKIFLSFLAKNALENPPPLTFFRDFMVERSGEH"
     assembly_gap    5325..5402
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5403..5672
                     /locus_tag="PK198_15855"
     CDS             <5403..5672
                     /locus_tag="PK198_15855"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HRF40267.1"
                     /translation="VAMVNNTFKRFEELAQREPGNRELYEQAADAYEILMRYRTLQGL
                     KQRDSGRFFNPSELSKMERILLRNSFRPIQDLQSLLKTRFQLGFF"
     gene            5692..6372
                     /locus_tag="PK198_15860"
     CDS             5692..6372
                     /locus_tag="PK198_15860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF40268.1"
                     /translation="MNFLQRLFSKPDYPEFWQRYLDAMQPPLSGNTPLREVTFAVFDT
                     ETTGLNPAKDKILSIGGVKVCQNEIHISHSIEIYLEQEAAPQQQDIAIHGIIPAAQAW
                     QQSSVQAVPSFVEWCGPAILTGHHVAFDVAIINQALQRQVGAQLRNRSVDTLQLAMRA
                     QGKSEHAAPGEWTLDRLASLYNIPVHDRHTAAGDAFITAILLMKLLHLLEKRGVKTLG
                     QLLNYRSL"
     gene            complement(6387..>7097)
                     /locus_tag="PK198_15865"
     CDS             complement(6387..>7097)
                     /locus_tag="PK198_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40269.1"
                     /translation="CGNGEIIARVQNVDNGGAAGVMMRETLAPGAKKAALKTRLTSFL
                     FREVRLTTNGNVTSQQFPAPTLPRWIRLVRNGSTFTAYHSTDGTNWSVTYTTTISMAQ
                     CIYIGIYAESVNGTTLTSAVFDNVSTVGSTSPLWAADHQVQAIAPERNDVEVFPNPAE
                     NEAFVRLGSELQNAGEVIVRLLDATGRPVWSGGFKPEEGDVLRIPLQALPAGVYVVSA
                     ESASGQGHKVKLVVQPSR"
     assembly_gap    7098..7149
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7150..7461)
                     /locus_tag="PK198_15870"
     CDS             complement(<7150..7461)
                     /locus_tag="PK198_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40270.1"
                     /translation="MLGVGGTYALLASDVIASMSDNCGTVSVQSIVPSILTCQHSGLT
                     VPVVVTVRDADGNTASCTAQVTVTDATSSPSGWTSSMVGNNAGSAVARSCDNSFILYT
                     SG"
     assembly_gap    7494..7530
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7531..>7853)
                     /locus_tag="PK198_15875"
     CDS             complement(<7531..>7853)
                     /locus_tag="PK198_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40271.1"
                     /translation="SPAPGSIVYQDTQINFTISDAAGNQSFCSFQVYLQDITPPAIQC
                     PAFFTIPKDASCQAVMPDLRNQITLNDACDPAPALVQIPAAGQVVTSLPFVSFLTSDA
                     SGNFN"
BASE COUNT         1745 a         1959 c         1961 g         1769 t
ORIGIN      
        1 cggaatggtc tgtacaattt tgtccgggtc ctcctgtgca acggccatta ccatgtcggt
       61 gccgatgtag cccggcgaga tggtgttgac ggtgatgcct ttgttggcta cctcgatggc
      121 caaggttttt gagaagccgt gcatacccgc cttggccgcc gcataattga cctgcccgaa
      181 ctgagcgcgc tgtgcattca ccgatgagat gttgatgatg nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cctacttcgt cgtagttggc
      421 cacatcggct ttggccatag caaaatcata gccttgcgct ttcatctcct gctgccagat
      481 gtcggctttg ttttgggtgc gataattggc aatcacaaaa aagccgtcgt cggccaattg
      541 cttgcacatg tcggtgccca aaccgcccac ggcgcccgtc accaatgcga tgtattttcc
      601 gtcttttgtc atgatgatgg attttacatg caaatatata gattattata tgtaaaatcc
      661 aaacttgtgg gtggatattt atcaaaaacc actactttgg ccgttcaaaa caaacgaaca
      721 ggcaatggca tctacacaaa tggtaacggc tacggccatc gtgctggccc tttacgcagc
      781 gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccga
      841 ctatgcggtg ggtagtattc agttttcgcc tattgtagtg ggactttctc tggcggcatc
      901 catcacgagc gcggccactt tcattatcaa tccggggttc attgcgctgt acggtttcag
      961 cgggatattg gcgctggcca tcgtgctgcc gatcgggttg tttttttcat tggtcattct
     1021 gaccaagagt tttcgcaagt acggtcagtc ggtaaaagcg ctgacgatgg cgcaatggat
     1081 tggcacacgt tacgacagca agggctttgc gctgttcttc gcgtttttgt ccctgctgct
     1141 gctcaccttc attgtactga tctgtgtggg catgacgaag gtaatgtcca atgctttgaa
     1201 cgtcaaagag atatatgttt tattaggctt ggtggttttt gtattcgggt acatgatgtt
     1261 cggcggggcc aattcgatgg tttacaccaa taccatccag gcggtgctga tgttgattgt
     1321 agctttcata ttgctggggt cggggttcga gcatttcaaa ggcggcgtca atggttttat
     1381 tgacaaattg gcggcaattg acccggcatt ggtgagcctt accaatcccg gcagcccttt
     1441 gtttcgtgat tattttgaga tcattttctg caatctgatt gtaggcatag ccattgtgtg
     1501 ccagccgcat attctcacca aaagcctgct gctgaaaaaa gagagcgacg tcaataggta
     1561 cttagttacc ggcattattg tggagatgat cttttttgca gtggtcattg cagggcttta
     1621 tgcgcgcctt tcatttcctg atctgaccac caacggccaa aagatcaata tggacggcat
     1681 catccccgcg tatgtagtgg cggaatttcc tgtttatgta ggcattttgg tcatcatggg
     1741 cctgctgtcg gccggtttgt ccacattgga ggggctgatt cagtcgcttt ccaccaccat
     1801 aacgaacgac atcattgctc ccgtgggcgg catcgaaaaa acatcaggta aattggtgtg
     1861 gatcaataaa gtcgtcatca ttgcgctggc cgtcactgcg gtgttgctga ccaaccacca
     1921 attgcagcat cccaacctga gcgtgggcat ttttgcacaa aacggcgtgt atgctttctt
     1981 ttcggctgca ttcatgccgg ttttgctggg ggttttcacc aaaaacaccc cgcgcatagc
     2041 gcccattgcg gcagctatta cggcggtcgt cgtgcatttc agcgtgtatt acatcggcat
     2101 cacgccgtat atgcagggag cggtgcgcaa tccggccata gccgccactt tggccatcct
     2161 gtcttcagcc gtggtgggca ctgcgctgta ctttttgttg agaaaaccgg aaacagcgcc
     2221 ttcacaggtc taatcatccg caccatgctc gactgggtct atccatatcc gctgaaaatg
     2281 gcgcatctgc cggagcaggt ttcggtggcc tacatagatg agggcgccgg gccacaaacg
     2341 ctcgtattcc tgcatggatt aggcagttat ctgaaaggat ggaccaaaaa cgtgtcggaa
     2401 ctcagccgcg attttcgctg cattgcgctc gattttccgg gctacggcaa gagcagcaaa
     2461 ggcgacctgc cttacagcat gacttttttt gccagggttg tgcgcgattt cattcagcgt
     2521 ttaaaccttc aaaatgtagt cctcgcaggt cactccatgg gcggtcaggt ggcggctacc
     2581 gttgcggtgc gctaccccga aatcgttcaa aaactcgtac tcatagcgcc ggcgggtttt
     2641 gaaaccttca ccgaaacaga ccgcgtgtgg attcgggcgg cctactcccc taccctgctc
     2701 aaagccatgc ccaccgatca gatcaggcgc aattttgagg tcaatttttc cagatttccc
     2761 gacgacgccg ggtttatgat tgacgaccgc atgatgatgc tgggcacgca ggcgtatgag
     2821 cagtattgcg agatgattcc ccgctgcgtc aacagtatgc tggacgagcc ggtttttgac
     2881 ctgctgccgc aaatcaaggc gccgacattg gtactctttg gagaaaacga cgtactgatt
     2941 cccaataagt tattgcatcc ggccctgaat ccggtcatgg tggcggaaac gggcgcccgc
     3001 cgaatacccg ctgcccgatt ggagctgctc cggccctgcg ggcactttgc gcaatgggaa
     3061 tgtgccgggg cggtgaatcg cgcggtcagg cggttttgcg ggtaggaacg ggttggtttc
     3121 gggttcaagg tttcgggttg gaacggctgc tggctgcaag ctgcaagccg cgcttaccgc
     3181 gccacggcgc ggctactgag tactgccgac cgcataccgg gtttcgggtt caaggtttcg
     3241 ggttccgccc gacgataaac tgcgcagcag atgactttcg ataaactgcc gaaggcagat
     3301 gacttccgat aaactgccaa aggcagattc cccctcacac caccttcgtc agcgtttgta
     3361 caatttcata atactctggt ttacagaggg cgtagtaaga gtttttacca tcgcgctgca
     3421 catccaccac atcctgattt ttgagaatgc gcaaatgatg cgatgcaatg gcctgctcga
     3481 ttttcagctt tttaaaaatt tcggtcaccg acaaacgctg gtgcgtatgc aacaaatcaa
     3541 cgatggcaat gcggatggga tgcgcaatgg cgcgcaggat ttcggtagag gcttccagaa
     3601 aggcttcgtc aaagtgtgtt ttcatcggac atgatttcta tgggatacct gatattttac
     3661 aatatcggca aaaatatagc cgagtaacaa atataagtat atataagttc atatatttac
     3721 cccacaaacc ttcgatcatg gaaaatacca ttcctgtacg gatttacgac tttttgaaag
     3781 gacatcctcc gttccatctt ttcgacaaaa acacattgct tgacctggcg gctcaggcaa
     3841 ttgttcaata ccgccccaaa ggagagcttg ttttcaacag ggagctatgc ccggcggatt
     3901 gatctttgtt ttaaaagaag gcgctattca cctgtttcac gaagagaagg gcgagcgaga
     3961 attggtggac caatgtgacg agggcgaact gttcggcctc cgacccttat tggccgacga
     4021 tgcgtatgcc ctcaccgccg ttgccgccga agaaacgctg ctgtacgcca ttcccgtgga
     4081 gtcttttcgg gaagcggcag aacaggcgcc gggggtgctg atgtacatcg cctccaattt
     4141 cgcatcgggc tggcaacgcc gaacgacgcc ggaatcgaag gggaaaattt ttgcgggcca
     4201 aaacaactcc gggcaagaag catttcaact tgttgaaata caatctattg aacgaagtaa
     4261 atcgcccgtt gtctgccgcc cggaacagcc gattcaggag gcagccggca ttatgaccga
     4321 aaaagaagtg ggttccatca tcgtggtcaa cgagcatcgg catcccatcg gcatcgtcac
     4381 cgataaggac ttgcgaaaaa aggccgccac cggcagggta cccatcagcg ccccggtctc
     4441 cgaaatcatg tcgcagcctg ttattaccgc ccatccctcc atcacggtag ccgatgtgca
     4501 aatgcgcatg gtgaagcaca aaatccccca cctctgcctc acagaaaacg gaaataacga
     4561 cgctcctgtg gtgggcgtac tcaccgagca tgatctgttg gtagtacagg gcaacaaccc
     4621 ggcggtattt ctgagggaaa tcaagcgggc acaaacgact tcggagttgc tccacattcg
     4681 cgaacgcgcc gagcaattgc tggaaaaata catccgccag gaggtggcca ttgcctacat
     4741 cgcctccatc atcaccgaga tcaacgatgc gctcattcaa cgcgtcatcc ggctggccga
     4801 acaacgcatg gacgcagagg ggcggcagcg gccgacggcc cgttattgct ggctggcatt
     4861 gggcagcgaa ggccgggagg agcaattatt gcgcannnnn nnnnnnnnnn nnnnntggtg
     4921 tttgaagacg tgccggaaca agcgcacgaa gccacaaaaa gctactacct gcaactaagc
     4981 ggctatgtaa ccaccatgct gaacgagtgc ggttttgaat actgcccggc agaaatgatg
     5041 gcctccaatc cgaattggtg cctgtcgttg agcgagtgga aagaacagtt ttccaaatgg
     5101 atgctggagc cggaccctca gtcggtgatg atgtgtacca tcttttttga ttatcgcccg
     5161 gtttacggcc tcagcgaatt gccggaagcc ttgaccgcgc atattttctc tatcatagat
     5221 caggaaaaga tattcctttc atttttggcc aaaaacgcat tggaaaaccc gccgccactc
     5281 actttcttcc gggattttat ggtggaacgc agcggagaac accannnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nngtggcgat ggtgaacaac acgttcaagc gctttgaaga actggcccaa cgcgaaccgg
     5461 gcaaccgaga actctacgaa caggccgccg atgcttacga aattctgatg aggtaccgca
     5521 ctttgcaggg cctgaaacag cgcgattccg gcagattttt caatccctcc gaattgagca
     5581 aaatggagcg catcctgctg agaaacagct tccggcccat tcaagacctg caatccttgc
     5641 tgaaaaccag attccagctt ggattttttt agaactcatc aacacatttt catgaacttc
     5701 ctgcaacgcc tcttttccaa accggactac ccggaattct ggcagcggta tttggatgcc
     5761 atgcagccgc ccttgtcggg aaatacgccc ttgcgcgaag tgacttttgc cgttttcgac
     5821 acagaaacca ccggcctgaa tcctgccaaa gacaaaattc tctccatcgg cggggtgaag
     5881 gtgtgccaaa atgaaattca catctcccac agcatcgaaa tatacttgga gcaggaagca
     5941 gcgccgcagc aacaagacat tgctattcat ggaattatac cggcagcaca agcctggcag
     6001 caatcctcgg tacaggccgt gccatctttc gtcgaatggt gcggccccgc catcctcacc
     6061 gggcatcatg tggctttcga tgtggccatc atcaatcagg cgctgcaacg gcaggtcggc
     6121 gcccaattgc gcaaccgaag cgtggacacc ctgcaattgg ccatgcgcgc ccagggtaaa
     6181 agcgagcatg ccgctcccgg agaatggacg ctggaccgat tagcctcgct gtataatatc
     6241 cccgttcacg acaggcatac ggccgccgga gacgccttca tcaccgccat attgctgatg
     6301 aaactgctgc acctgctcga aaaaagaggt gtcaagacat tgggccaatt actgaattac
     6361 aggtctttat gaatataaaa ccatcctcac ctcgatggct gcaccaccaa tttcacctta
     6421 tgcccttgcc ctgatgccga ttctgcactc accacataaa cgccagccgg caaagcttgc
     6481 aacggtatgc gcagcacgtc cccttcttcg ggtttgaacc cgccggacca taccggccgg
     6541 ccggttgcat ccaataagcg gacgatgact tcgccggcat tttgaagctc ggaacccaag
     6601 cgaacaaaag cctcattttc ggcaggattg ggaaacactt ccacatcatt ccgctccggc
     6661 gcaatagctt gcacttgatg atcggcggcc caaagcggcg aagtagaacc tacggtggat
     6721 acattgtcga acacagccga ggttagggtc gtaccgttga cgctttcggc atagattcct
     6781 atgtaaatac actgcgccat agaaatcgtg gtagtgtaag taacactcca gttggtaccg
     6841 tcagtagagt gatacgcggt gaaagtagag ccgttgcgca ccaggcgaat ccatctgggc
     6901 agggtgggcg ccggaaattg ctgcgaagtg acattgccgt ttgtagtgag cctcacctcc
     6961 ctgaaaagga aagaagtaag tcttgttttc aaggccgctt ttttagcgcc gggcgccaga
     7021 gtttctcgca tcattacacc cgcggcgcct ccgttgtcca cgttttgtac gcgggcaatg
     7081 atttctccgt tgccgcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnng cccgaagtgt agaggatgaa gctgttatcg cagcttcggg ccacggcgct
     7201 gcctgcattg tttcctacca ttgagcttgt ccaaccagaa ggcgaactgg tagcatccgt
     7261 taccgtcacc tgcgcggtac aggatgcggt gttgccgtcg gcgtccctca ccgttaccac
     7321 cacaggtacc gtcaggccgc tatgctggca agtcaaaata gaaggcacaa tgctctgtac
     7381 agacaccgtg ccgcagttgt cgctcataga tgcaatgaca tccgaggcca acaatgcgta
     7441 agtgcctccg acaccaagca tcgccatagc gggcttgcag gcaactgtcg gtgnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attgaaattc ccggatgcgt cggaggtgag
     7561 aaacgacacg aagggcagcg atgtaacgac ctgcccggct gccggtatct gcacgagcgc
     7621 aggggcaggg tcgcaggcgt cgttgagtgt aatctggttg cgaagatcgg gcataaccgc
     7681 ctgacaggaa gcatctttgg gaatggtaaa gaaagcgggg cactgaatgg ccggaggagt
     7741 tatatcttgc aaatagacct gaaaagagca gaaactctga ttacctgccg catcggaaat
     7801 ggtgaagttg atctgcgtgt cttgataaac aatggaaccg ggagccggcg act
//
LOCUS       DAORLF010000210         7829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5031_length_7829_cov_2.292771, whole genome shotgun sequence.
ACCESSION   DAORLF010000210 DAORLF010000000
VERSION     DAORLF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_7829_cov_2.292771"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="secA"
                     /locus_tag="PK198_15880"
     CDS             complement(<1..690)
                     /gene="secA"
                     /locus_tag="PK198_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRF40272.1"
                     /translation="MFSFIGKSLKKMFGTKHDRDVAGYSGAIDRIHEVTEELKGLSND
                     ALRNKTLEFRSRIAEYLAEIDAEIQQLRQQAIDTEDIHAKEDFFEEVDKLEEERNKAL
                     EEVLREILPEAFAVVKETARRFTENETLTVTATEHDADLAAVKSYVTIEGGKAFWKNK
                     WTAAGADITWNMVHYDVQLIGGMVLHDGKIAEMATGEGKTLVATLPAYLNGLSGRGVH
                     VVTVNDYLARRD"
     gene            799..1515
                     /locus_tag="PK198_15885"
     CDS             799..1515
                     /locus_tag="PK198_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40273.1"
                     /translation="MTKRLAFGLFLSIFILGACRNDAPRDIRNYYFPLRQLTEGLVYE
                     YRADDSLTPAYWHYRSILGKEGHFLTSTYYEQELEPLQMTREEMVSNGMLLRDLRLFA
                     TDTASLARTTTVQIEHGNVFPFQVTDSLGVFLYKVSWTDEEGQQITLTRNRRFLGDTS
                     FHFEGRTWPGVVFSLIESIELDHPQQGYFEQQLQGREGYAHELGLFFYGKAPKGQAPI
                     IFSLRDRYTMEELEKKANKR"
     gene            complement(1581..2438)
                     /gene="gldN"
                     /locus_tag="PK198_15890"
     CDS             complement(1581..2438)
                     /gene="gldN"
                     /locus_tag="PK198_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRF40274.1"
                     /translation="MKIALSAIIAVLTLLATPLVAQFPGNIMTESGETDKSKPLDDIV
                     EKRTIMEKRVLPYDPVREADIFFEKRIWRVIDVREKMNLPFAYPERPFFSILLDAAKS
                     GEITVYSQENDKFTVPMTPEEVASIGSSVDTVTIIDPETYEEVIQIVTNEINPADVKR
                     FRVKEIWFFDRETSTLNVRILGIAPLYDERDANGEFKYEKNLFWVYYPEARQVLARER
                     VFNIGNDASPMTWEDIMEMRFFSSYIFKESNVFDRRLSEYLSGVDLLMEGEKIKNEIF
                     NMEHDLWSY"
     gene            complement(2594..4222)
                     /locus_tag="PK198_15895"
     CDS             complement(2594..4222)
                     /locus_tag="PK198_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRF40275.1"
                     /translation="MSIPKEPRQLMINIMYLVLTAMLALNVSAEVMNAFFTLDDGNKA
                     SIGTVSSQLDNTVKSVNELLNEPAKAKFKPIGPAMKQVQDASARFNTYVNQLRDKLID
                     EAGHKDGKVNDEDYKESYGKRVPKGMKNKDVTTRMLVQEGEGEKLKKNILDTRQELID
                     IYTKLVKENGQAFGLKPAEIDSRIESIAQNMPFKIDDETWKKTKDKKSWSDFKFRQMP
                     LAAVLPLMSQMQADLKSSEASMVNSMAELTGGRIVEIDQFFPVVQAEKSYVIAGEPFN
                     AEVSIGSYSSTLDPSNVVVTVNGSSLRVGEDGKAKYSTTTSSTGPQTLNLGVRVTNPL
                     TGEVKEGKGTYTYEVGRRSVAVSADMMNVFYIGVENPVSISAAGVPTSELRVSASGGG
                     INLRSSGTGKYVATVSTQGEATITVSGGGLKASPFQFRVKRIPDPVAKLSGKVGGEMG
                     SGEFRAQGGLAAILENFDFNAKCEIQGYDLTYQAARQDAVTSTNPGGRFNDKSSRLVQ
                     QAKPGDRFFFENVRARCPGDQAGRRINDLVFKIK"
     gene            complement(4280..4894)
                     /locus_tag="PK198_15900"
     CDS             complement(4280..4894)
                     /locus_tag="PK198_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRF40276.1"
                     /translation="MSFLKTKGFKYVKNFIIGVGASAVLFGALAKIQSWEGADFWLTI
                     GMLTEAGIFLFLGLIGPEPDYYWHKLYPGLDDYNAPIAPLAAGPVAAQTKAVAPLNGE
                     VVERQLGGMLMELQTMSKSLGSLKALQEVDFSGTKDQIKAMSNFYAKLNEAMADLSDT
                     VEDTKQYKDQLSELNKNISGLNTVYSSLNNVYGNVITAMAGVRG"
     gene            complement(5047..6312)
                     /locus_tag="PK198_15905"
     CDS             complement(5047..6312)
                     /locus_tag="PK198_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF40277.1"
                     /translation="MKRLSKISLLLLVLAAAACGKRESGQLTGVLERPNWKGINPYGM
                     VYVPSGTLHIGPSDQDVSNTFVQRQRSISIQGFYMDDTEITNNEYRQFVYWVRDSLAH
                     SYLDHFIEDEVTGDQRIDWEYKIDWEDETLQDLYYQGDDRFAGRQEYDVDKFWFEYEW
                     YDWQGAAHDDHRGHDRNNRGKERTSFIKKKTVKIYPDTLVWVRDFSYSYNEPMTRNYF
                     HHPAFDDYPVVGVDWHMANAFSYWRTKLWNAYEAEKTGANSEDFRLPTEFEWEWAARG
                     GHDLAPYPWGGVYIRNAKGCLLANFKPGRGNYPEDGGLYTVKADAYFPNEYGLYNMAG
                     NVAEWTISAYEANSYSFIHDLNPDIRYDATDEDPEAYKRKVVRGGSWKDVMFFLQNGT
                     RSYEYQDTTKSYVGFRNVLTYLGRSINDF"
     assembly_gap    6686..6706
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6707..7471)
                     /locus_tag="PK198_15910"
     CDS             complement(<6707..7471)
                     /locus_tag="PK198_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40278.1"
                     /translation="MKKLIFLFLMLIVLAPAQAQLEWAPVGAKYYYSTWWGFSGETGV
                     TVVEVAGDTMVQGKNCRYFRRASGTVPWTAACQAFYDITYSENRQVYFLDEYTQDFKL
                     FYDFNKMPGEVWDVTLCGYEPWCQVDTLRMQVAAVTDTVLNNVTVRKMNLTSYILSYP
                     TYTFQWVVYEGIGNIPVMHFFWERCITVEAGILNLRCYESPTHGIFNFVGGQPCGVYT
                     AAGEAQAAVEQLRMYPNPAREWLNVELPQGGRGQLAL"
     gene            complement(7485..>7829)
                     /locus_tag="PK198_15915"
     CDS             complement(7485..>7829)
                     /locus_tag="PK198_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40279.1"
                     /translation="QQAWLLKLDAYGCLVPGCHLSTAAEEPAQARFELRLYPNPASEY
                     LNFYLRAPAAAGREVSFRIFDASGALLQEFAGVASDATYIVPVWDWPAGTYVLQCVGQ
                     GQVLAVEKFVKQ"
BASE COUNT         1821 a         2057 c         1922 g         2008 t
ORIGIN      
        1 gtcgcgtcgg gccaggtagt cgttcaccgt taccacatgc acgccacgac cggaaaggcc
       61 attgagatat gcaggcagag tggccaccag cgtcttacct tcaccggtag ccatttctgc
      121 gattttgcca tcgtggagta ccataccgcc gatgagctgc acatcgtaat gcaccatatt
      181 ccaggtaata tcggcgccgg cagccgtcca tttgtttttc cagaaagcct taccgccttc
      241 gatggtcaca tagcttttta cggctgccag atcggcatca tgttccgtgg ctgtaacggt
      301 cagcgtttca ttttctgtaa agcgccgggc tgtttctttt acaaccgcaa aagcttcggg
      361 caggatttcg cggagtactt cttcaagcgc tttgttgcgt tcttcttcca acttatctac
      421 ttcttcgaag aagtcttctt tggcatgaat gtcttctgta tcaatggctt gttggcgcag
      481 ttgctgtatc tcggcatcta tttcggccaa gtattccgcg atgcggctcc gaaattcgag
      541 cgttttattt ctcagggcat cgttgctcaa gcctttgagt tcttcggtca cttcatgtat
      601 gcgatcaatg gcgccggaat agccggccac gtcgcggtca tgtttggtgc cgaacatttt
      661 tttgagggat tttcctatga agctgaacat atatcttgtt ttgaaagagt tgtatggttg
      721 aacgggcaaa ggtaatactt ttgcccccgt gtcggaaacg ataatgcctt tcatcacatt
      781 ttataccaaa aacagaacat gacaaaacgt ctcgcttttg ggctttttct ctccattttt
      841 atcctcggcg cctgccgcaa tgacgcgccc cgcgatattc gcaactatta tttccccctc
      901 aggcaactca ccgaagggct tgtgtatgaa taccgtgccg acgactcgct gacgccggcc
      961 tattggcact accgctccat actcggcaaa gagggccatt ttcttaccag tacttattac
     1021 gagcaagagt tggaaccttt gcaaatgacc cgcgaagaaa tggtttccaa cggtatgtta
     1081 cttcgggatt tacggctttt tgctaccgat actgccagcc tcgcccgcac cacaaccgtg
     1141 cagattgagc acggcaatgt tttcccattt caggtcaccg attcgctggg cgttttttta
     1201 tacaaagttt cctggaccga tgaggaaggc cagcaaatca ccctcacccg caaccggcgc
     1261 tttctgggcg atacgagttt tcacttcgaa gggcgtacct ggccgggagt ggtgttttct
     1321 ttgatagaat ctattgagtt ggatcacccg caacaaggtt attttgagca acaattgcag
     1381 ggtcgcgaag gctacgccca tgaattaggg ttgttttttt atggaaaagc gcctaaggga
     1441 caagcgccca ttattttttc actccgcgac cggtacacga tggaggaatt agaaaagaag
     1501 gctaataagc gttagaaaca caaaagccgc tcgcgataca cacacgagcg gcttttgcct
     1561 atgaaaatca gactctgcca tcaatacgac cacaagtcgt gttccatatt gaagatttca
     1621 ttcttgattt tttctccctc catcagcaaa tccacgccgc tgaggtactc ggacaaacgc
     1681 cggtcaaaaa cgttggactc cttgaagata tatgaagaga agaatcgcat ttccatgatg
     1741 tcttcccaag tcatgggcga agcatcgttg ccgatattga atacgcgctc acgagccaac
     1801 acctgacgtg cttcgggata atacacccaa aacaggtttt tctcgtattt gaactcgccg
     1861 ttggcatcgc gttcgtcata caacggagca atgccgagga tgcgaacatt gagcgtagaa
     1921 gtttcgcggt cgaagaacca gatttccttc acacggaaac gcttcacgtc tgccgggttg
     1981 atttcattgg ttacaatctg aatgacctct tcgtaggttt ccgggtcaat aatagtaacc
     2041 gtatccacac tggagccgat ggaagctact tcttccggcg tcatcggaac ggtgaatttg
     2101 tcgttttcct ggctgtacac ggtgatctcg ccggacttgg cggcatccaa cagaatgctg
     2161 aagaacgggc gttccgggta agcgaaggga aggttcattt tctcgcgcac atcaataacg
     2221 cgccagatgc gcttttcgaa gaagatgtct gcttcgcgaa cggggtcgta aggcagtacg
     2281 cgcttttcca tgatggtgcg cttttcgacg atgtcgtcca gcggtttcga cttgtccgtt
     2341 tcaccggatt ccgtcatgat gttgccggga aattgggcga ccagcggagt tgccaacaac
     2401 gtcagaacgg cgatgatggc agagagagca attttcatag ttgtttccag tttttgatat
     2461 tttttttgcc cgggcgaaac ctttgctcag agtaagcctc cctgcaaaat cggttccgcc
     2521 cggacaaaat ttttgaaagt gcgacaattg aacggctcag accagccgac tattacaact
     2581 ttaacactaa aaattacttg atcttgaata ccaagtcatt gattcggcgt cctgcctggt
     2641 cgccggggca acgcgccctt acgttttcaa agaaaaagcg gtcgccgggc ttggcctgct
     2701 gtaccaaacg agaagactta tcattgaaac ggcctcccgg attggtggag gtcacagcat
     2761 cctgacgagc agcctgatag gtcaagtcat acccttggat ttcacacttg gcgttaaaat
     2821 caaagttttc aagtatggct gccagaccgc cctgcgctct gaattcgcct gatcccattt
     2881 caccccctac tttgccgctc aatttggcga ccgggtcagg aatgcgcttc acacggaatt
     2941 ggaaagggct ggctttcaag ccgccgccgc taacggtgat ggtcgcctcg ccttgtgtgg
     3001 atacagtagc aacatatttg cctgtacccg aactacgcaa attgatgcct ccaccacttg
     3061 ccgatacgcg cagttcactg gtaggaacgc ccgctgccga gatggaaaca ggattttcca
     3121 caccgatgta aaatacgttc atcatatcgg cagaaacggc gaccgagcga cgacctacct
     3181 cataggtgta agtgcctttg ccttctttca cttcgccggt gagcggattg gttacgcgca
     3241 cgccgagatt cagcgtttgc gggccggtag agctggtggt agtgctgtac ttagctttgc
     3301 cgtcttcgcc tacacggagt gaagaaccat tgacggtcac cactacattc gatggatcaa
     3361 gcgtagaaga gtagctgcct atggatactt cagcattgaa cggttcgccg gcaatcacat
     3421 agctcttttc agcctgtacc accgggaaga actggtcaat ttctacgatg cgaccgccgg
     3481 tcaattccgc catgctgttg accatggaag cttctgagct tttcaggtcg gcctgcatct
     3541 gagacatgag cggaagtaca gcagccaaag gcatctggcg gaatttgaaa tccgaccagc
     3601 tttttttgtc tttcgttttc ttccaggtct catcgtcaat tttgaaaggc atattttgag
     3661 cgatgctttc aatacgcgaa tcaatttcgg cgggcttgag gccgaacgcc tgaccatttt
     3721 cctttaccaa tttggtgtaa atatcaatca gttcctgacg cgtatccaga atgttcttct
     3781 tgagcttttc gccttcgcct tcctgcacca gcattcgggt ggtaacatcc ttgtttttca
     3841 tgcctttggg cacgcgctta ccatatgact ccttgtaatc ctcatcattt actttgccgt
     3901 ctttgtgacc ggcttcgtca atgagtttgt cgcggagttg attcacatag gtattgaagc
     3961 gggccgaagc atcttgaact tgcttcatcg caggcccgat cggtttgaat tttgctttgg
     4021 caggttcatt gagcaattcg ttcacacttt ttaccgtgtt gtcgagctgg ctcgacacgg
     4081 tgccgatgct ggccttgttg ccgtcatcaa gggtgaagaa tgcgttcata acctccgccg
     4141 agacgttgag ggccaacata gccgtgagta ccaagtacat gatgttgatc atcaactgcc
     4201 gcggttcctt tggaatagac atattgcgag agttttttac agtttaacaa tacaggtgta
     4261 ttttccaaaa gcgcgaagat caaccgcgaa cgcctgccat agctgtgatc acattgccgt
     4321 aaacgttgtt aagcgagcta tagaccgtgt tgagaccgct gatgtttttg ttcagttcgc
     4381 tcagttggtc tttgtactgc ttggtgtctt ctactgtgtc gctcaggtca gccatagctt
     4441 cgttgagttt tgcgtagaag ttgctcatgg ccttgatttg gtctttggtg ccggagaagt
     4501 ccacttcctg caaagccttg agcgaaccca agctcttcga catggtttgg agttccatga
     4561 gcataccgcc caactgacgt tctaccactt cgccgttcag cggggccaca gccttggttt
     4621 gtgcggctac cggacctgct gccaaggggg caatcggtgc gttgtagtcg tccaaacccg
     4681 gatagagttt gtgccagtag taatcgggtt ccgggccgat caagccgagg aacaagaaga
     4741 tgcctgcctc agtaagcatg ccgatggtca gccagaagtc ggcgccttcc cagctctgaa
     4801 tttttgccag tgcgccaaac agtaccgcag aagcgcctac gccgatgata aagtttttga
     4861 cgtacttgaa acctttggtt tttaagaaac tcattgttac agcttgttta aaggttgtta
     4921 aaaaagaagt tacagtcgga taatgcagat tttgaaaaaa gtaacaccga tggtgtaaaa
     4981 aaatgcaaaa tatgggtact gccgttacag caatacccat atcccaatca tctgagtgat
     5041 gaaaatttag aaatcattga tggaacggcc caagtaggtg agaacgttgc ggaaaccgac
     5101 ataagacttg gtagtgtcct gatactcata agagcgggta ccgttctgaa ggaagaacat
     5161 cacgtctttc caggagccgc cgcgcaccac tttgcgcttg tatgcttccg ggtcttcgtc
     5221 ggtagcatca taacggatat ccgggttgag gtcgtgaatg aacgagtaag agttcgcttc
     5281 gtaggcagaa atagtccact ctgccacatt gccggccata ttgtaaaggc catattcgtt
     5341 ggggaagtag gcatctgctt ttacggtgta gagaccgccg tcttcggggt agttgccgcg
     5401 accaggcttg aaattggcca aaagacaacc ttttgcattg cgaatataaa cgccgcccca
     5461 gggatacgga gcgagatcgt ggccgccgcg agccgcccat tcccactcaa actcggtagg
     5521 caaacggaag tcttcgctgt tggcgccggt tttctcagcc tcataagcat tccagagctt
     5581 ggtacgccag tagctgaagg cattggccat gtgccagtcc acgccgacta caggataatc
     5641 gtcaaaagcg gggtggtgga agtagttgcg cgtcatcggc tcgttgtacg agtaggagaa
     5701 atcgcgtacc cacaccaagg tatcgggata aattttgacg gtttttttct tgatgaaaga
     5761 ggtgcgctct ttgccgcgat tgttacggtc gtggccgcgg tggtcgtcgt gagcggcgcc
     5821 ctgccagtcg taccattcgt attcaaacca gaacttgtcc acatcgtact cctggcggcc
     5881 tgcaaagcgg tcgtcgccct gatagtacaa atcctgaagc gtttcatcct cccagtcaat
     5941 tttgtattcc cagtcaatgc gctgatcgcc ggtcacttca tcctcaatga agtgatcgag
     6001 atagctgtgg gcgagcgagt cgcgcaccca gtacacaaac tgacggtact cattgttggt
     6061 gatttccgtg tcgtccatgt agaatccctg aatagagatg gagcgctggc gctggacgaa
     6121 tgtgttgctc acgtcctggt cgctgggacc gatgtgcagg gtaccggagg gtacatacac
     6181 cataccgtag ggattgatcc ctttccagtt gggacgttcc agaacgccgg tgagctggcc
     6241 tgattcgcgt ttgccgcagg ccgccgccgc cagaacgagc agcagcagag aaatcttaga
     6301 cagtcttttc atgtttacac ccagattttc ctttagaatg aaaaagtttt tgagaaagtg
     6361 cgcccaaaga tataggcttt cccagaaaca caaatgttaa agtttgcttt cctgcaaaaa
     6421 accttccaat aggcgctttt cagacacagg cttcaacgcg gattcggaaa ggggtatttt
     6481 gtggcaggat tttttttaac aaccatcatc ctccgtcgaa ctcggtgcaa acctgaccgt
     6541 caccgcctac tccctcacaa acctccccag cgccgcagct ccgccctcgc ttcggtacag
     6601 cagcagatac atacccgtcg gcaaggccga cacatccagc ggccaggatt ccggcacagc
     6661 atctaagtat tgcgttttca gcagcnnnnn nnnnnnnnnn nnnnnncagc gccaactgcc
     6721 ctctgccgcc ctgcggcaat tccacattca gccactcccg cgccggattg gggtacatgc
     6781 gcaattgctc caccgccgcc tgcgcctcgc ctgccgccgt ataaaccccg catggctgcc
     6841 cgcctacaaa attgaaaatg ccgtgcgtgg gagattcgta gcaacgcagg ttcagaatcc
     6901 ctgcttctac cgtaatacac cgctcccaaa aaaagtgcat gacgggaata ttgccgatgc
     6961 cctcatatac cacccactgg aaagtgtatg taggatacga taaaatatat gaagtcaggt
     7021 tcattttccg aaccgtcaca ttgttgagta ccgtgtctgt tacagcggct acttgcattc
     7081 ttaaagtatc aacctgacac caaggctcgt agccgcacaa agtcacatcc cagacctcgc
     7141 cgggcatttt gttgaaatcg taaaacagtt taaagtcctg tgtgtattcg tcaagaaaat
     7201 aaacttgtcg gttttccgag taggtgatat cataaaaagc ctgacatgct gccgtccacg
     7261 gtacagtccc ggaagctcgc ctgaagtacc tgcaattttt tccctgcacc atggtatctc
     7321 cggcaacctc tactacggtt acgcccgttt ctccactgaa tccccaccaa gtggaataat
     7381 aatacttcgc ccccaccggc gcccactcca actgcgcctg cgccggagcc aggacgatca
     7441 gcatcaaaaa gaggaaaatg agttttttca tggttgtgtg ttttttactg ttttacaaat
     7501 ttctccaccg ccagcacttg gccctgcccc acgcattgca gcacataagt gccggcgggc
     7561 cagtcccata ccggtactat ataggtggca tccgacgcga ccccggcgaa ctcctgcaac
     7621 agcgcccccg aagcgtcgaa aatgcgaaac gatacctcgc ggcctgccgc agcgggcgcc
     7681 cgcaaataaa aattgaggta ctcgctcgcc ggattgggat acagccgcaa ctcgaaccgc
     7741 gcctgcgccg gctcttccgc cgccgtactc aggtggcagc ccggcaccaa gcagccgtaa
     7801 gcgtccaact tgagcagcca ggcttgctg
//
LOCUS       DAORLF010000211         7825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5036_length_7825_cov_2.043887, whole genome shotgun sequence.
ACCESSION   DAORLF010000211 DAORLF010000000
VERSION     DAORLF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_7825_cov_2.043887"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PK198_15920"
     CDS             complement(<1..1151)
                     /locus_tag="PK198_15920"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF40280.1"
                     /translation="MKRSHIMLSLVAIGLLVAAFFPKNQHQPEKDALLIRSVLTHLEQ
                     LHYHPKTLNDDFSQKLYDTYLERLDFGKRFLTQKDVTKLAPYRSHLDDDLRAGNIEFF
                     DLSVDLLNAGVQKVSEYYKEILDKPFNFENNQGVAAAKPGKGNPVSTPANAPLIELDG
                     KKRSFAASEEELYNYWEKYLQYEVMTRLSDKLDDQEEKGEAADKKSIKELEAEARAET
                     LKSMNQWFERMQKVKRAERFGYFLNALTNLFDPHTDYLAPIDKQNFNIRFSGRLEGIG
                     AQLQTDGDFTKVILVVVGGPAWKQKELEEGDIITKVAQGNNTPVDVAGMDINEVVSMV
                     RGEKGTEVRLTVKKKDGSVKIIPIVRDIVIMEEGFAKSLLLDGAVEGEK"
     gene            complement(1355..2677)
                     /locus_tag="PK198_15925"
     CDS             complement(1355..2677)
                     /locus_tag="PK198_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF40281.1"
                     /translation="MRTIFLHQRFFASLGLLAALFVLGFALPPLFFIAQALLVVWVAV
                     LALDAWLLYNGSFELNGHRRLPAVFSLGDPNPVYVHLENRSSIPLRCQVIDELPKQFQ
                     IRDFVQHVSLRPDEAQEIRYELTPVVRGEYGFGALNLFATSPIGLLERRVRIEAEAVI
                     PVFPSIIQMKKYELRAFDRMAQFNGLKRMRRIGHSYEFEQIKNYVRGDDYRSINWKAS
                     SRRAELMVNQYEDERAQQVYCIIDKSRAMRLPFEGLSLMDYAINTSLVISNIALQKHD
                     RAGLVTFSDKIGAAIKADSRSGQLNKILHALYREKERPLESNYELLFQATRKLISGRS
                     LLLLFTNFESSYALDRVLPLLRRINNMHLLVVVFFENTEIRALTRQPAETTEDIYRQT
                     IAEQFLAEKSQMVNKLRQFGIQAVLTRPEDLSINTINKYLELKSRGLI"
     assembly_gap    2785..2932
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2933..>3307)
                     /locus_tag="PK198_15930"
     CDS             complement(<2933..>3307)
                     /locus_tag="PK198_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40282.1"
                     /translation="QAALFEVMEEYQVTVDGQTYPMQFPFFVMATQNPIEQEGTYKLP
                     EAQLDRFLVRIILDYPGLGEEKTILRRFQNDFNISLRDSVKPVLSPEDIRACRELVQQ
                     IYIKEELLDYIATIVHHTRNNG"
     assembly_gap    3308..3360
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3361..3759)
                     /locus_tag="PK198_15935"
     CDS             complement(<3361..3759)
                     /locus_tag="PK198_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40283.1"
                     /translation="MTTDDMNLPEDAGLPTPEEMVPANLDNWQRKRLDLSRIASGVQR
                     IREELAKVIVGQYDGIDMMLAAIFSGGHILVEGVPGIAKTLMAKLLARTLNTGFSRIQ
                     FTPDLMPSDVIGTTVFNVKTSEFSFNKGPVF"
     assembly_gap    3836..3865
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3992..5290)
                     /locus_tag="PK198_15940"
     CDS             complement(3992..5290)
                     /locus_tag="PK198_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40284.1"
                     /translation="MDRRKIIMIVTITAVIAGLALLFWKQWKRPVSWNEHYAAQSKDP
                     YGAYVAFQTLKGSTDKKNFKTLSDSLSGKLPIDSSQNARYIFIGKTMYLSKSSLDALI
                     EFVAAGNDAFISANHLPYALLEEIYPNYCETDQETDYYYGSGWEGFSYTEDSLVRLNL
                     AHPDLQLKKDVPYRRKYYQRFVKGNWGFMEERHFLCHQEGAMTALGHLNGSLVNYARI
                     PYEEGHFYIHSTPLAFTNYHLLRSEALNYAERAFSHLRTGPVYWDEGSKNPRSNALDT
                     TPPSRSLSSDSPLQYILSQPPLAYAWYLLLLLGVLYLLVRSRRRQRIVPVLESNANTS
                     LEFVSTIGRLYFLQNDHKQLCLQKMRLFQQFIRERYGLQSRESDEIFLAKLAAKSEVP
                     RELIDKLFLIYYNIQSSNIVTEYTLAQLHGLIEEFHRTCK"
     gene            complement(5439..6260)
                     /locus_tag="PK198_15945"
     CDS             complement(5439..6260)
                     /locus_tag="PK198_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRF40285.1"
                     /translation="MKKHRLIWLLVCAAPLWLFAQAPPQIEEYPVEETYDMEEVVVSS
                     STPSDKLREENVRMEKLDREAWQKATEGLDYSGSREKKKRDLNGNGSGGGKDREGGGK
                     RRKNSGIFSSDSGFFNSDVGITILKILVIIIAAVALFFLIRSLMGLRGPRNRKLKTAE
                     LLGLDIAEIEDRFQELNLDDYIRQAIERGDYPLAVRLYYLAALKALSARELIQWKKDK
                     TNLSYLLELRGQPQLPAFQEITHIFEWVWYGQGGIDRQEFERVQPKMKGFLEGMG"
     gene            complement(6276..>7825)
                     /locus_tag="PK198_15950"
     CDS             complement(6276..>7825)
                     /locus_tag="PK198_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRF40286.1"
                     /translation="ARRELALSFGIFALSMLIGALSSAMDSEFVNVILSEEYVEMTKR
                     NIESGDPMAVYKGRGQLSMSMGITVNNLMVAFLTFATGAFFTLGSIVVLIRNGIMVGA
                     FQYFFIERGLFWESFLTIWIHGTLEISAIIIAGAAGITVGKGLVFPGAYRRMQAFQRS
                     ARRGLKIMVGIAPIIILAGFIEGFLTRFTETPDVVRGAFIAACFAFVIFYFVWYPWSK
                     AVKGFTSPVRDAQIPPDSNLLIDMNVIKSNGDIFSETFTLAKKNAGRLFIGSLVVALA
                     YAAVAFPLAGKAPAAVFQFPQGFMAMLYKVSGFFRQADLLFLPLVGIAAMLAWSLIAF
                     RWVDKETGLARKLRFTDILMPLPGVLLVWLLFFSNDWYTWLLLLFAAQMALLWAYTAY
                     RERMDPIRAIGRMFGHGWPIWAYAGFVFDDAVGERAVFCRHRFCPALDVLGCIDLGSQ
                     LARSEYATVFGSVAHGVACFHHRLPFCVVRIGLRPALPQSVGNARGSGPVAQNTKHRY
                     YLQYTRT"
BASE COUNT         1821 a         1956 c         1987 g         1830 t
ORIGIN      
        1 attttttcgc cttccacagc gccgtcaagc aggagcgact tggcaaaccc ttcctccatg
       61 atgacgatgt cgcgcacgat ggggatgatt tttacagagc cgtctttttt cttcaccgta
      121 aggcgcactt cggtaccttt ttcgccgcgc accatagaca ccacttcgtt gatgtccata
      181 ccagctacat ccaccggagt attattgccc tgcgccactt tggtaatgat gtctccttct
      241 tccaattctt tttgcttcca ggcagggccg cccactacta cgagaatcac cttggtaaaa
      301 tccccgtccg tttgtagttg tgcgccgatg ccttcgagac gaccggagaa gcggatgttg
      361 aagttttgct tatcaatggg cgccagatag tcggtatgcg gatcgaaaag attggtcagt
      421 gcattgagga aatagccgaa gcgttccgcg cgctttactt tttgcatacg ctcaaaccac
      481 tgattcatag acttgagcgt ttcggcgcgg gcttccgctt ctaattcttt gatgcttttc
      541 ttgtcggcag cctcgccttt ttcttcctga tcgtcgagct tgtcggacag gcgggtcatc
      601 acctcgtatt gaaggtattt ttcccagtag ttgtacaact cttcttcgga ggcggcaaag
      661 cttctttttt tgccgtccaa ctcaataagc ggagcattgg caggcgtgct caccgggttg
      721 cctttgccgg gtttggctgc agctactccc tgattgtttt caaaattaaa aggcttatca
      781 aggatttctt tatagtattc gctcactttc tgaacgcctg cattgagcaa gtccaccgaa
      841 agatcaaaaa attcgatgtt gccggcacgg agatcgtcat caaggtgaga gcgataaggc
      901 gcaagtttgg ttacatcttt ttgagtgagg aatcgcttac cgaaatccaa cctttcgagg
      961 taggtatcgt aaagtttttg cgaaaaatcg tcgttgagcg tcttagggtg gtaatgcagt
     1021 tgctccagat gcgtcaacac ggaacgaatc agcaatgcgt ctttttcggg ctggtgctgg
     1081 tttttgggaa aaaatgccgc taccaacagg ccgatggcca ccaaagacag catgatgtga
     1141 cttctcttca tggtctcgaa tgtttgtcta aatggatttt tcatcattgt catcgcgttt
     1201 ttttaaagtc gaaagacagt tgcaacgccc aatacaacgc atcgcaggaa aaaaaagata
     1261 ccacagaaca gaaaaaactt acgcgataat cgtgccaaag ttatgcttta actaattttt
     1321 aggaaaaggc agggtacaaa aattgcctcc gctttcaaat caagcctctc gacttcagtt
     1381 ccaaatactt attgatggta ttgatggaga ggtcttcggg gcgggtgagt acagcttgaa
     1441 tgccgaactg tcgcagcttg ttcaccattt gagatttctc tgccagaaac tgttcggcga
     1501 tggtttgccg gtagatatcc tcggtagttt cggcgggttg ccgggtgagg gcgcggattt
     1561 ccgtattttc aaaaaacacc accaccaaca ggtgcatgtt gttgatgcgg cgcagcagcg
     1621 gcagcacgcg atctaaagca tagctgctct cgaaattggt aaacaacagc aacaaactgc
     1681 gccccgaaat gagcttgcgt gtggcctgaa acaacagctc atagttcgat tccagcgggc
     1741 gttccttctc gcggtacagg gcgtggagga ttttgttgag ctggccgctg cggctgtcgg
     1801 ctttgatggc ggcgcctatt ttgtcggaaa aagtgaccaa gcccgcgcgg tcgtgttttt
     1861 gcaaggcaat gttggaaatg accaagctcg tgttgatggc gtaatccatc aggctcagtc
     1921 cctcgaaagg caggcgcatg gcccggcttt tgtcaatgat gcaatacacc tgctgtgcgc
     1981 gctcgtcttc gtattggttc accatcagtt cggcccggcg gctgctggcc ttccagttga
     2041 tggagcggta atcgtcgccg cgcacatagt ttttaatctg ctcaaactcg tagctgtggc
     2101 cgatgcggcg catgcgtttg aggccgttga actgcgccat gcggtcgaaa gcgcgcagtt
     2161 cgtatttttt catctgaatg atggacggaa aaaccggaat caccgcctcg gcttctatgc
     2221 gcacgcgccg ttccagcagg ccgatgggcg aggtggcaaa caagttgagg gcgccgaagc
     2281 cgtactcgcc gcgcacgacg ggcgtcagtt cgtatcggat ttcctgcgcc tcatcggggc
     2341 ggagcgagac atgttgcaca aaatctcgta tctgaaactg cttcggcaat tcgtctatca
     2401 cctggcagcg caacggaatg gaagacctgt tttccaagtg tacatatacc ggattggggt
     2461 cgcccaatga gaacaccgcc ggcagccggc gatgcccgtt gagttcgaag ctgccgttgt
     2521 acagcaacca ggcatctaaa gccagcaccg ccacccatac caccaacaag gcctgcgcta
     2581 taaaaaacag cggcggcagg gcaaaaccga gtacaaacaa ggctgccagc aggcccagcg
     2641 aggcgaaaaa gcgttggtgc agaaagatgg ttctcataat ggccggccct tatcggggca
     2701 cttctatttt ggaaatgata tcgtctatga cgtttttggt ggtgaagccc tccatttctc
     2761 gctccggcgt gaggatgatg cggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgccgtt
     2941 gttgcgggta tgatgcacga tggtggcgat gtagtccaac agttcttcct tgatgtaaat
     3001 ctgctgcacc aattcgcgac aagcgcggat gtcttcgggc gacaaaaccg gcttcaccga
     3061 atcgcggagg ctgatgttga aatcgttttg gaagcggcgc aaaatggttt tttcttcgcc
     3121 caggccggga taatcgagga tgatgcgcac caaaaagcgg tccaattggg cttcgggcag
     3181 cttataggtg ccttcctgtt caatggggtt ttgggtagcc atcacaaaga aaggaaactg
     3241 catggggtag gtttggccgt ccaccgtcac ctggtattct tccatcacct caaagagggc
     3301 cgcctggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 aaaaacaggc cccttgttga acgagaactc agaggttttg acattgaaca cggtggtacc
     3421 gatcacatcg ctgggcatga ggtcgggcgt aaactgaatg cgcgaaaatc ccgtgttcag
     3481 cgtgcgggcc aacagcttgg ccatcagggt tttggcaatg ccgggcacgc cttctaccaa
     3541 gatgtgtccg ccggaaaaga tagcggccag catcatgtct atgccgtcgt attggcccac
     3601 aatgactttg gccaattctt cgcgaatgcg ctgcacgccg gacgctatgc ggctgagatc
     3661 caatcgtttg cgttgccagt tatccaaatt ggcaggcacc atttcttcgg gggtgggcag
     3721 cccggcatct tcgggcaggt tcatgtcgtc ggtggtcata agttacattg tttgtatgga
     3781 gagtttcctt tagctaccag cccttagctt ctggctgtta gcttagactt ccaaannnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnaattc attttcaaaa gcgtatttag aaaattcccc
     3901 aagcggaaaa tctaatttct gaacaaagaa caagctaatg gctatgagct tacagcttgt
     3961 ggctttggag gatattaaaa aacaactgca ttcacttaca ggtgcgatga aattcctcga
     4021 tcaggccgtg caattgcgcc agcgtgtatt cggtcacgat gttggaagat tgaatattgt
     4081 agtaaatgag aaacaatttg tcaatgagtt cgcgcggcac ttctgatttg gcagccaatt
     4141 tggccaaaaa gatttcgtcg ctttcgcgcg attgcaggcc gtagcgctcg cgtatgaatt
     4201 gctggaaaag ccgcattttc tgaaggcaca attgcttgtg gtcgttttgc agaaaataca
     4261 gccgcccgat agtggacaca aactccagcg aggtattggc attcgattcc agcacgggca
     4321 caatgcgctg ccggcggcgg gatcgcacca acagatacag cactcccaac agcagcaata
     4381 agtaccaggc ataagccaat ggcggctgcg acaggatgta ttgcaaagga ctatcggaag
     4441 acaaactgcg cgacggcggc gtagtatcca aggcgtttga ccgcgggttt ttgctgccct
     4501 cgtcccaata taccggcccg gttctcaggt gcgaaaaagc ccgctcggcg tagttcaatg
     4561 cttcgcttcg caacaggtgg tagttggtaa aagccaaagg ggtgctgtgt atgtagaaat
     4621 ggccttcttc ataagggatg cgggcataat tgaccaagct gccgttgagg tggcccaggg
     4681 ccgtcatggc gccttcctga tggcacaaaa agtggcgttc ctccataaag ccccagttgc
     4741 ccttcacaaa acgctggtag tatttccgcc ggtaaggcac gtcttttttc agttgcaggt
     4801 cgggatgagc cagattgagg cgcaccaaag aatcctcggt ataggaaaac ccctcccagc
     4861 cggagccgta gtaataatcg gtttcctgat cggtttcgca gtagttgggg taaatttcct
     4921 ccagcagcgc ataaggcagg tggttggccg agatgaaggc atcgttgccg gcggccacaa
     4981 attcgatgag cgcatccaga ctgctcttgc tcaggtacat ggttttgccg atgaaaatat
     5041 agcgggcatt ctgcgaactg tcaatgggca gtttgcctga caagctgtcg gacagcgttt
     5101 tgaaattctt tttatccgtg ctgcctttga gcgtttggaa ggctacgtaa gcgccgtagg
     5161 ggtctttgct ttgggccgca taatgttcgt tccacgacac cggccgcttc cattgcttcc
     5221 aaaaaagcaa ggccagcccc gcaatgacgg cggtgatggt aacgatcata atgattttgc
     5281 gacgatccac ggcgatgaca aaattttaca aatgttcttc aaaaacagac agatacgggc
     5341 gaaatccgaa gatgcgattg atctatttct tattttactt tttgccctta aagtaaaata
     5401 agactgttcg ttattttact ttttgacaat aacacgccct accccatccc ctccaaaaag
     5461 cccttcattt tgggctgcac ccgttcaaac tcctggcgat ctatgccgcc ctgcccgtac
     5521 cacacccact cgaaaatatg ggtaatttcc tgaaaagccg gcaattgcgg ttggccgcgc
     5581 agttccagca gatagctcag gttggtttta tctttcttcc actgaatcag ttcgcgggca
     5641 gagagcgctt tgagggccgc cagatagtag agcctcactg ccagcgggta atcgccgcgt
     5701 tcgatggcct gacggatgta gtcgtcgaga ttgagttcct gaaagcggtc ttcgatttcc
     5761 gctatatcaa gccccagcaa ttcggctgtt ttgagcttgc gattgcgcgg gccgcgaagc
     5821 cccatcaggc tgcgaatgag aaaaaagagc gctaccgctg caatgataat gaccaatatt
     5881 ttgagtattg taatgcccac atcgctgttg aaaaaaccgg aatctgagga gaaaatgccg
     5941 ctgtttttgc gccgtttgcc gcctccctcc cgatccttgc cgccgccgct tccattgccg
     6001 ttcaggtctc tttttttctt ttcccggctg cccgaatagt ccaaaccctc ggttgccttt
     6061 tgccaggcct cgcggtccaa tttttccatc cgcacatttt cctccctgag cttgtcggac
     6121 ggcgtgctgc tcgacacgac gacttcctcc atgtcgtaag tttcttcgac aggatactcc
     6181 tctatctgcg gcggagcctg cgcgaacaac cacagcggcg ccgcacacac aagaagccat
     6241 atcagccggt gttttttcat ttcacttatt ctttctcaag tcctcgtata ttggaggtaa
     6301 tacctatgtt ttgtattctg cgcaacaggg ccggagcctc gtgcatttcc aacagactgt
     6361 ggtagagcag ggcgcaaccc gatgcgtaca acacaaaaag gaagccgatg atgaaaacat
     6421 gcaacaccgt gagcaacact gccgaaaact gttgcatact cgcttcgggc aagttgacta
     6481 cccaggtcaa tacatccaag tacatccaaa gcagggcaga atcggtgacg gcaaaaaaca
     6541 gcacgctcac caacagcatc atcaaagaca aacccagcgt acgcccatat tggccaccca
     6601 tgaccgaaca tgcgccctat ggcccgaatg ggatccatgc gctcccggta ggcggtatag
     6661 gcccaaagca gagccatttg cgccgcgaac aacaacaata gccaggtgta ccagtcgttg
     6721 ctgaaaaaca ggagccatac caacaacacg ccgggcaggg gcatgagtat gtcggtaaac
     6781 ctgagcttgc gcgcaaggcc ggtttctttg tccacccatc tgaaagcaat cagcgaccag
     6841 gccagcatgg ctgcgattcc gaccaaaggc agaaacagca gatcggcctg ccggaaaaag
     6901 cccgacactt tgtacaacat ggccatgaaa ccctgtggaa actggaatac cgcagccggg
     6961 gccttgccgg cgagagggaa cgccacagcg gcatacgcca gtgcgaccac gaggctgccg
     7021 atgaacagcc ttcccgcatt tttcttggcc agagtgaagg tttctgaaaa aatatcaccg
     7081 ttcgacttga tgacattcat atcaataagc agattgctgt cgggcggtat ctgggcgtcg
     7141 cgtacaggtg aagtgaaacc cttgacagcc ttggaccagg ggtaccacac aaaataaaaa
     7201 atgacgaacg cgaaacaggc cgcaatgaat gcgcctcgca ccacatcggg cgtttcggtg
     7261 aagcgggtga ggaagccttc gatgaagcct gccaatatga tgatgggtgc tatgccgacc
     7321 atgattttga ggccgcgccg ggcagagcgt tggaatgcct gcatacggcg gtaggccccg
     7381 ggaaacacca aacctttgcc caccgtgatg cccgctgcgc cggcaatgat gatggccgat
     7441 atttcgaggg tgccgtgtat ccatattgtg agaaaggact cccaaaacag acctcgctcg
     7501 atgaaaaagt attgaaacgc tcccaccatg atgccgtttc ggatgagtac cacgatggaa
     7561 cccagcgtaa aaaacgcgcc ggtggcaaag gtgagaaagg ccaccataag attgttgacg
     7621 gtgatgccca ttgacatgct caactgcccc cggcctttat acaccgccat aggatcgccg
     7681 gattcaatgt tgcgcttcgt catttccaca tattcctcgc tgagaatgac attgacaaac
     7741 tcgctgtcca tagccgacga cagggcgccg atgagcatgg agagtgcaaa tatgccgaaa
     7801 gaaagcgcca gttcgcggcg ggctt
//
LOCUS       DAORLF010000212         7825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5037_length_7825_cov_1.812227, whole genome shotgun sequence.
ACCESSION   DAORLF010000212 DAORLF010000000
VERSION     DAORLF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5037_length_7825_cov_1.812227"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..848
                     /locus_tag="PK198_15955"
     CDS             270..848
                     /locus_tag="PK198_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HRF40287.1"
                     /translation="MTTAIKLPLRSLNESVIRDLKEKYPEAEISVELHQDRNKSPLSE
                     AAFWEMISLLDWSKEGHDEEIAEPVIAKLAAGPIRHIFEFADILSEKLYALDARKYSI
                     HIGEDSWAPDQYFSVDNFLYARCCVVANGKDAYNSALHNPAQMPKDLTFEALLYLPLE
                     AYERKTGNRYDYTPAYPIETYSNQAGWSEETT"
     gene            863..1185
                     /locus_tag="PK198_15960"
                     /pseudo
     CDS             863..1185
                     /locus_tag="PK198_15960"
                     /inference="COORDINATES: protein motif:HMM:NF026989.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1189..1989)
                     /locus_tag="PK198_15965"
     CDS             complement(1189..1989)
                     /locus_tag="PK198_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF40288.1"
                     /translation="MNYKLLFPTYRNRYLFVRNQLRQLAAEQRSFSSALNLGTGEGDY
                     DVMIAPFCQSLFACDINEQDVAFAQNLNREVPNLHYGVENALELSFPDASFDLLISVD
                     VLEHVGRPERMMEEVGRVLQPGGLALITFPSLEFPFTYDPINRILSLFTDKKIPQGAY
                     AFGHEYLVLGSDFRLWAERNGLEVLQEYNLSGALVALAEMYWTGIIQRIFKANATNVS
                     ENKREKAMRPSTQEPALAALTDAFIALDKALFGRGKRSVGKGFVLRKR"
     assembly_gap    2113..2243
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2302..>3759)
                     /locus_tag="PK198_15970"
     CDS             complement(2302..>3759)
                     /locus_tag="PK198_15970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF40289.1"
                     /translation="KGLTKDFQVIGKYALLVAGAAIGSYILAILTWPAALADPLGHPF
                     KALSEFSQLGIKIRLLFQGENVMSDKTAWYYPVVWIVKTIPFSVLFGFLGAVILSPIM
                     LQRRQPLAVLLMFFAAIFPVFYVIYKDSVLHDGWRHLMFVYPALVVCATLFWTTLERL
                     VSRWNWGKYAIFGLLALLSVDAVAFIARNPTLSYVYFNPLGGGLKGALGNYETDYWGT
                     GVKQALDWMEKEGILSENMKDTVVIGTTFFYPMLYQTGKYKGMVKPVYVRFGQRYSES
                     WDYGVFPSRFIRGPHLRSGSWPNSKAIHTISANGVPIVAIEKDADKWAWQGEKAAKEQ
                     NWPDAIAAFEKETKAHKDNELAWQGLANALLNSGKFQEAVAAADESIKIAPDNEQGLF
                     LKGLAYLNLGDAAKAAEALENNVKVNDESPYAHYYLGLIYQQSGDIDRAITNAVRAVE
                     LNGRFKQAYELAAALYEQKGDANTAAAYRNAAAQL"
     assembly_gap    3760..3880
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3881..4513)
                     /locus_tag="PK198_15975"
     CDS             complement(<3881..4513)
                     /locus_tag="PK198_15975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF40290.1"
                     /translation="MSKKKPTAQRPSPAKAAAVRKSAPAVEKWPTVSEQDGLLKKVFW
                     GLAALALAVMMLVAPATGVNGDDEFQNDYSQKLVAYYSTMGKDTSALFIEKGNMHNYG
                     GFFDLLAGAANAALGNDVLDPGYHAMRHYFNALFGLLAMVFIGLLAKEIAGWRAAVIA
                     LLIAFFSPRFFGHSMMNPKDIPFAAGFAIALYYMVRSLRKLPEISWKDALG"
     gene            4621..5826
                     /locus_tag="PK198_15980"
     CDS             4621..5826
                     /locus_tag="PK198_15980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF40291.1"
                     /translation="MSNTTSLLSQRALRMEESATLRMAAMARELKAKGHQVIDLSLGE
                     PDFDTPDHIKEAAKKALDDGYTKYTPVAGLLELREAICTKFKRDNGLEFLPNQIVVSN
                     GAKQCIANVCMAMLDPGDEVIILSPYWVSYAEIVKVAEGEPVIVHAGIEQEYKATAAQ
                     VEAAITERTKLLLFSSPCNPTGSVFSEEELQAIAEVVSGHERIHIISDEIYEYINFSG
                     RHHSIGTIESVKDRTITINGFSKGFAMTGWRLGYLGAPKWIADACNKIQGQFTSGANS
                     FGQKAAAYALLADMKPTDEMRHAFEKRRNMVVELLGKIPGIKANMPEGAFYSFPDISA
                     LFGKRCGDTVVNNPDDFCEYLLETEYVAAVSGSAFGDDHCFRISFAASEEQLREAVIR
                     IERAVRELK"
     gene            6123..>7825
                     /locus_tag="PK198_15985"
     CDS             6123..>7825
                     /locus_tag="PK198_15985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRF40292.1"
                     /translation="MTTFDYIVFAMYGALIVSLGLYMSRTKKGTEKTAEDYFLASKSL
                     PWWAVGTSLIAANISAEQMIGMSGQGFGMGLAIASYEFMAAVALLVVAKYFLPIFLKK
                     GLYTIPQFVEQRFSKDLKTILAIFWIALFTFVNLATVLLLGALAMDTIIGTGDGSLLL
                     ISMIGLAGVAIAYSVYGGLSAVAWTDVLQVGLLILGGLITNVLALQHVPPDASAITGI
                     KHVLDTASDKFHMILPKGHPSYGDLPGIAVLVGGMWVANLYYWGFNQYIIQRALAAKS
                     LPEAQRGLVFAGFLKLLIPLIVVVPGIVAWVMYSQPEGTAIIDGVRDSLTVNKPYAPN
                     DNAFPWLIRSFVPAGLRGLVVAALAAAIVSSLASMMNSVSTIFTMDIYKEYFNKQSSE
                     RNSVNVGRITALVAIVIGIIMAVALQNSKGIFNVIQEYTGLVSPGILAIFMMGLFWKK
                     ASNKSALWAAVLSILFAFAFKLGKIMGALDDKGQIADGASGLEQFFGSLSQIPWMHQM
                     GLTFLFTCGIIAAVSYAGGGKDDPKAIELGKGVFKTDAKYNIGAFTILLILAFIYAIF
                     W"
BASE COUNT         1847 a         2052 c         1934 g         1740 t
ORIGIN      
        1 gctcgtttga aaaaacatac cgctgcgatt tcggcgacct cagcggcgca aaattcatcg
       61 gcgtctttag tgaaggtccg cagcaggctg cctattttgc cgatctgctt atctggaaaa
      121 attatcagtc gtttgattga gattgttgct gaaacggggg gcggaaggag gacgcagttt
      181 ccaaacttcc ttctgaaccc gaattttcag aaaaaaggga gttacttctg ccagaagaag
      241 ttgttatctt tgaccgaaat tgaatcatca tgaccactgc catcaagctg ccgcttcgct
      301 ctctgaacga gtccgttata cgagacttga aagaaaaata tcccgaagcg gaaataagtg
      361 tggagctaca tcaggacaga aataaatcgc ctttgagcga agctgctttt tgggaaatga
      421 tctccctgtt agactggagc aaagagggcc atgacgagga gatagcagaa ccggtcatcg
      481 caaaattggc agcaggcccc attcgacata tttttgagtt tgcagatatt ttgtctgaaa
      541 aattgtacgc ccttgatgcc cggaagtatt ccatacatat tggggaagat tcctgggcac
      601 ccgatcagta tttttcagtg gataactttt tatatgcacg ttgctgcgtg gtagccaacg
      661 gtaaagatgc gtacaacagc gcactgcaca atcccgctca aatgcccaaa gacctcacat
      721 ttgaagcgtt gctgtacttg ccgttggagg catacgaaag gaaaacagga aatcgctatg
      781 attatacccc tgcatatccc atagaaacgt acagcaatca ggcaggctgg agtgaagaaa
      841 cgacataagg aaaccaactc caatgaattt tcacggcaat tcaaggcaaa acacaaaaga
      901 acaccatctc tacgctatcc atgacaaaga ggaagcagat gtattcaaat acggcatcag
      961 tgacaagccc attggagagg acggctattc acagagaatg cgggacaggt ggatttcctg
     1021 aacagggctg tgggatgggc gcgatacttt gcagaggtct tgattcgggg cattgccggg
     1081 aacgccaaag ctcgtcagat agaagacgat ttcatcaatg cttacaaaga aatctatgcc
     1141 agaaaccctc gtggaaatgt caaaaagact tcaaagcagc attaaatttc accgcttcct
     1201 caacacaaac cccttgccca ccgaacgttt ccccctgccg aacagagcct tgtccaatgc
     1261 gataaaagca tcggtgaggg ctgctaacgc cggttcctga gtggaggggc gcatcgcttt
     1321 ttcccgttta ttttcagaca cattggtggc attggccttg aaaatgcgct gaatgatgcc
     1381 cgtccagtac atctccgcga gggccaccaa cgcgccgctc aggttgtact cttgcagcac
     1441 ttccaagccg ttgcgttcgg cccaaagccg gaaatcgctg cccagcacta agtattcatg
     1501 cccgaaagca taggcgccct gcggtatttt tttgtcggta aacaaagaca aaatccggtt
     1561 gatggggtcg taagtgaacg gaaactccag cgaggggaag gtaatcagcg ccagcccgcc
     1621 gggttgcagc acgcgcccca cctcttccat catgcgctcg gggcggccca catgctccag
     1681 cacgtccacc gagatgagca ggtcgaaaga ggcatcggga aaggagagtt ccagcgcgtt
     1741 ttcaacgcca tagtggagat tgggcacctc gcggttgagg ttttgggcga aagccacatc
     1801 ctgctcgttg atgtcgcagg caaacagcga ttggcaaaac ggcgcaatca tcacgtcgta
     1861 gtcgccttcg ccggtgccga gattgagcgc ggaagagaag ctgcgttgct ccgctgccaa
     1921 ttggcgcaac tgattgcgta cgaacaggta gcgattgcgg taggtaggaa aaagtagttt
     1981 ataattcata agtaaatgtg ctaatttgac aatatgctaa tcccgatatt cgggacaagt
     2041 tatgctgatt gccaatcgag agcgtccaaa atagcatcaa ttaatctgac tcaattactt
     2101 aaatggtcat gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnncagaatc ggaaatccag aatcggaaat ccagaatcgg
     2281 aaatcggaaa tccggaaatc gtcaaagctg cgcggcggca ttgcgataag ctgcggcggt
     2341 attggcatcg cctttttgtt cgtaaagagc ggcggccaat tcataagcct gcttgaagcg
     2401 gccattgagt tccacagcgc gtacggcgtt ggtaatggcg cggtctatgt cgcctgattg
     2461 ttggtaaata agccccagat agtaatgcgc gtaaggcgac tcatcgttca ccttcacatt
     2521 gttttccaat gcttcggcag ctttggccgc atcgccgaga ttgaggtagg ccaagccctt
     2581 gaggaacaag ccctgctcgt tgtcgggcgc tattttgatg ctctcgtcgg cagcggcaac
     2641 ggcctcctga aacttgcccg aattgagcag cgcattggct aagccctgcc aggccaactc
     2701 gttgtctttg tgcgctttgg tttctttttc aaaagcggca atggcgtccg gccagttttg
     2761 ttctttggcg gccttttcgc cctgccaggc ccatttgtcg gcatcttttt cgatggccac
     2821 aatgggcacg ccgttggcac ttatggtgtg aatggctttg ctgttgggcc aggaccccga
     2881 cctcaaatgc ggcccgcgaa tgaagcgcga gggaaacacc ccgtagtccc atgactcgga
     2941 atagcgctgc ccgaaacgca catacacggg tttgaccatg cctttgtact tgccggtttg
     3001 gtacaacatc ggataaaaaa acgtggtgcc gatgacgacc gtatccttca tattttcgct
     3061 caggatgccc tctttttcca tccagtccaa cgcctgtttg acgccggtac cccaataatc
     3121 ggtttcgtaa ttgcccagcg cgcctttcag cccgccgccc aatggattga aataaacata
     3181 ggacagcgta ggattgcgcg cgatgaaagc cacggcgtcc acgctgagca atgccagcag
     3241 gccgaagatg gcatacttcc cccagttcca acgagatacg agccgttcga gcgtcgtcca
     3301 gaacaaggta gcgcacacca ccaaagccgg atacacaaac atgaggtgcc gccagccgtc
     3361 gtgcagtaca gaatctttgt aaatgacata aaacacaggg aaaatggctg caaaaaacat
     3421 cagcaatacg gccaaaggct gccggcgttg gagcataatc ggcgataaaa tgacggcgcc
     3481 caaaaatccg aacagcaccg aaaacgggat cgtttttaca atccacacca ccggatagta
     3541 ccaggccgtt ttgtccgaca tcacattttc gccctgaaag agcagcctga ttttgatgcc
     3601 caattgcgaa aactcagaca gcgctttgaa cggatgcccc agcggatcgg ccaatgcagc
     3661 cggccaggtg agaatggcta ggatgtaaga accgatggcg gcgccggcta ccaggagggc
     3721 gtattttccg ataacctgaa agtctttggt caaacccttn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccgagcgcg tctttccaac
     3901 tgatttccgg caacttgcgc aggctccgca ccatataata taaggcgatg gcaaaacccg
     3961 ccgcgaaggg aatgtctttg gggttcatca tcgaatgacc gaaaaagcgc ggcgaaaaaa
     4021 aggcgatgag cagggctatg acagcagccc tccagccggc aatttctttg gccagcaagc
     4081 ctatgaacac catagccagc aagccgaaca gcgcgttgaa gtagtgccgc atagcgtggt
     4141 agccgggatc gagcacgtcg ttgcccagcg cggcattggc ggctccggcc agcaaatcga
     4201 agaagccgcc gtagttgtgc atattgcctt tttcgatgaa cagcgctgag gtgtctttgc
     4261 ccatggtgct gtaataggcc accaattttt gcgagtagtc gttttgaaac tcgtcgtcgc
     4321 cgttcacgcc ggtagccgga gccaccagca tcatcaccgc gagggccaga gcggccagac
     4381 cccagaacac ctttttcagc aggccgtcct gttcagaaac ggtgggccat ttttctaccg
     4441 caggcgctga tttgcgtacc gcggcggctt tggcgggcga cgggcgttgg gcagttggtt
     4501 ttttcttact catcgaaaca ggaatatgtg ttttgaacgg cgcaaagata gcaattctca
     4561 aaagtagcgg gtaatgggaa aatggctact tttgccgctg cttttttcaa accttacaac
     4621 atgtccaata ccacctctct cctctctcag cgcgccctcc gcatggagga atctgccacg
     4681 ctacgtatgg ctgccatggc ccgcgaactc aaagccaaag gccatcaggt gatagacctg
     4741 agcctcggcg aacccgattt cgacacgccc gaccacatca aagaggccgc caaaaaagcc
     4801 ctcgacgatg gatataccaa atacactcca gtagccggtt tgctggaatt gcgcgaagct
     4861 atttgcacca aattcaagcg cgacaacggg ctggagttcc tccccaacca aatcgtggtg
     4921 tccaacggcg ccaagcaatg tatcgccaat gtgtgcatgg ccatgcttga tccgggcgac
     4981 gaggtcatca tcctctctcc gtattgggtg tcttacgccg agattgtaaa agtagctgag
     5041 ggcgagccgg ttatcgttca tgccggcatc gagcaagaat acaaagccac cgccgcacag
     5101 gtggaagcgg ccatcacgga acgcaccaaa ttgctgctgt tttcctcgcc ctgcaacccc
     5161 acgggttcgg tgttcagtga ggaagagttg caggccatcg ccgaggtagt aagtggccac
     5221 gagcgcattc acatcatttc ggatgagatt tacgaataca tcaatttttc cggtcgccac
     5281 cacagcatcg gcacgataga gagcgtcaaa gaccgcacca tcaccatcaa cggcttctcc
     5341 aaaggttttg ccatgacggg ctggaggttg ggctacctcg gcgcgcccaa gtggattgcc
     5401 gatgcctgca ataaaatcca gggtcagttt accagcggcg ccaattcttt cggccaaaaa
     5461 gccgccgcct acgccctctt agccgacatg aagcccactg acgagatgcg ccacgccttt
     5521 gaaaaacgcc gcaacatggt ggtagaactg ctgggcaaaa tccccggcat caaagccaac
     5581 atgcccgaag gcgcttttta cagcttcccc gacatcagcg ccttgttcgg caagcgctgc
     5641 ggcgataccg tcgtcaacaa tcccgacgac ttctgcgagt acctcctcga aaccgaatat
     5701 gtggccgccg tttcgggcag tgctttcggc gacgaccact gcttccgcat ctctttcgcc
     5761 gcctccgaag agcaactccg cgaggccgtc atccgaattg aacgggccgt gcgcgagttg
     5821 aaatgaccgg gatagtgaag gggtgactca gccaaaccaa gccgtagtcg ccccttcact
     5881 aaacccgtac agcccatgaa catgagtatt tttaaccgtg catcgaccgg cgaggcagac
     5941 tcatagctcg caggctcgta gcttttggca gggtattaaa tagtaacaaa gaatatttat
     6001 accttcgccc atcgcatttg ccaaattatt caccttcttg catctgtcgc actctgtaat
     6061 tttttaccaa aacctgcgct acaacaactc gtcatttttt catccaacca taaccttctt
     6121 gtatgaccac ttttgactat atcgtatttg ccatgtacgg cgcactcatc gtgagtttgg
     6181 gcttgtatat gtccagaacc aaaaagggca cggaaaaaac ggctgaggat tactttttag
     6241 ccagtaaatc attgccctgg tgggcggtag gcacctccct tattgccgcc aatatttcgg
     6301 cagaacagat gatcggcatg tcggggcagg gcttcggcat gggtttggcc attgcatcct
     6361 acgagttcat ggcagcggtg gccctgttgg tagttgccaa gtattttctg cccatatttt
     6421 tgaaaaaagg actttacacc atcccgcagt ttgtcgaaca gcggtttagc aaagacctca
     6481 aaaccatctt agccatcttt tggatagccc tcttcacttt cgtcaacctc gctacggtgt
     6541 tgttgctggg tgcgctggct atggatacca tcatcggcac aggcgacggc agcttgttgc
     6601 tcatcagtat gataggtttg gccggcgttg ccattgccta ttctgtatat ggcggtttgt
     6661 cggctgtggc ctggacggat gtgctgcaag tcggattgct gattttgggc ggattaatca
     6721 ccaacgtgct ggccctccag cacgttccgc ccgacgccag cgccatcacc ggcatcaaac
     6781 atgtgctgga taccgcctcc gacaagtttc acatgatttt acccaaaggg cacccctcct
     6841 acggcgactt gcccggcatt gccgttttgg taggcggcat gtgggtggcc aatttgtact
     6901 attggggctt caaccagtac atcatacagc gcgcattggc ggccaagagc ttgcccgaag
     6961 cgcagcgtgg cttagtgttt gccggtttcc tcaaactcct cattcccctc atcgttgtag
     7021 tacccggaat cgttgcctgg gtcatgtact cccagccgga aggcaccgcc atcattgacg
     7081 gcgtgcgcga tagcctcacc gtcaacaagc cgtatgcccc caacgacaat gccttcccct
     7141 ggctcatccg ttcctttgta ccggcagggt tgcgcggctt ggtggtagcg gctttggcag
     7201 cagccatcgt gtcatcgctg gcctccatga tgaactccgt gtccaccatt ttcaccatgg
     7261 acatttacaa agaatatttc aacaaacagt cttcggagcg caattcggtc aacgtgggcc
     7321 gaatcacggc tttggtcgcc atcgttatcg gcatcatcat ggccgttgcc ctccaaaact
     7381 ccaaaggcat tttcaacgtc attcaggagt acaccggctt ggtcagcccc ggcattttgg
     7441 ccatctttat gatgggcctg ttctggaaaa aagcctccaa caaatcggcg ctctgggcgg
     7501 ctgtcttgtc cattttgttc gcatttgctt tcaaattggg caaaatcatg ggcgctctcg
     7561 acgacaaagg ccaaatagcc gacggcgcct ccggtctgga gcaattcttc ggcagcctca
     7621 gccagatacc ctggatgcac caaatgggac ttacgttcct cttcacctgc ggcatcattg
     7681 ctgccgtgag ctacgccggc ggcggcaagg atgatcccaa agccattgaa ttgggcaaag
     7741 gggttttcaa aacagacgcg aaatacaaca tcggcgcctt caccatcctg ctgattctgg
     7801 catttattta cgccattttc tggta
//
LOCUS       DAORLF010000213         7810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5048_length_7810_cov_2.076854, whole genome shotgun sequence.
ACCESSION   DAORLF010000213 DAORLF010000000
VERSION     DAORLF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_7810_cov_2.076854"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="PK198_15990"
     CDS             complement(<1..1826)
                     /locus_tag="PK198_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF40293.1"
                     /translation="MKKHTLIEQIAEKLRIIPNLHKEGETPVDRLAPGSELEQWDNWT
                     ENDAKSWPRIKKRNYTIVPTTCFNCESACGLTAYIDKETMQVQKFEGNPYHPASRGRN
                     CAKGPATVNQLTDPDRILYPLKRVGKRGEGKWERVTWDQVLDDIAGRLNKALREIASH
                     VGRPGHEGYMDRVLQAWNVDGHNSHTNICSSGARFGYALWYGHDRPSPDHANADFILL
                     ISAHLESGHYFNPHAQRIIEGKMKGAKLAVLDPRLSNTASMADYWMPSYPGTEAAVLL
                     AIAKILLEEGMYNRDYMENWVNWDEYLAAKHPDKPRTFEQFIASVVEEYAEYTPEFAE
                     QESGVTAEMIREVAKGIGKAGTRLSTHNWRSAASGNLGGWAVARALHFLNVITGSVGT
                     EGGTAPNTWNKYHPKFFDSPPAQKFWNELHFPNEYPLAFFEMSFLLPHMLKENRGKMD
                     VYFTRVFNPVWTYPDGFSWIEALCDEEKFGLHIALTPTWNETAYYADYVLPMGLASER
                     HDLNTYETHAGVWIAFRQPVLREAARRMGKPVTFTYEANPGEVWEEDEFWIELSWRMD
                     PDGSLGIRKHFLSPYRPGEKINIDEYYQYVFEHTKGLSATAA"
     gene            complement(1847..3472)
                     /gene="nrfD"
                     /locus_tag="PK198_15995"
     CDS             complement(1847..3472)
                     /gene="nrfD"
                     /locus_tag="PK198_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF40294.1"
                     /translation="MKFGFIIDNRKCIGCHACTTACKSEHDVPVGVFRTWVKQVEKGE
                     FPHTRRLFSVMRCNHCTDAPCVEICPVEALYFRDDGIVDFDKNRCIGCKSCMQACPYD
                     ALYIDPETHTAAKCNYCAHRIDVGLEPACVNVCPEHAIISGDMDDPNSEISVMLGREQ
                     VSVRKAAKGTVPNLFYINGDEASLNPVATARQSDYFWNEQSHGVGHFAQKTEKLAFSE
                     GNLVNALLDKNGSAVAAGDLAKGTPAKSIDLLMGQPRRVYDAPVKGILWGWEVSAYVV
                     TKAVSAGAVLVALMGSLFRLSSVSDTTFWWALGLGLFFLMATGALLVKDLDRPDRFLN
                     VLLRPQWKSWLVRGGYTISVFGGLLALLAALMFFDLKGGYTPVLYAVTLAAATMTAVY
                     TAFLFAQAKGRDFWQSPSLALHMLVHSFMAGAAVFLIVNLITNEGIVWTDYLRNTLII
                     GLVVNMALMVLEFVTTHPTQEAKLTVKMILKGRYRNMFWIGAVLVGNVLPLALLLLGK
                     DAMMSAAASALVMIGMYVTEKIWVEAPQRVQLV"
     gene            complement(3636..5159)
                     /locus_tag="PK198_16000"
     CDS             complement(3636..5159)
                     /locus_tag="PK198_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40295.1"
                     /translation="MQLIRSLTQAEKRHFRIFARRHQSADGLLFVQLFDVLEKKRDYD
                     EAAVLKKIPALKKEQLPNLKAHLYRQLLTSLRLLSKNRNEDIALRENIDYARVLYDKG
                     LYRPALDILAKAKDRATGGQFKSIALEIIEFEKHIEGQYITRSIEGRARELSRQSAEL
                     TREIAVTHGFSNLSLEMYGLYLQLGFARNREDHERVKAFFRERMPVETFQELDFLGRI
                     YYCQAHVWLHHICQEFPACYRYAQKWTELFEASPAMSALHPAMYLKGIHNLLSALFNS
                     LYDVRFEAVWEQLETFPERVGVGQNKNLEGLYTLYRYIHLINLHYLRGDYERGVHIVP
                     EMNELIERNPYNWDQNRILQFHYRLACLCFGNGDQNGAIDFLNKIIQQKTTDYRNDIH
                     CFARILHLIAHYELGHIELVEHLAKSVYRFLLHIENLQAVQLEILRFIRRVHGRPTEG
                     LRSEFETLHTKLSLLMNDPIERRPFMYLDILSWLESRMEGVPIQQVIQKKRVIRTMR"
     gene            complement(5224..6072)
                     /locus_tag="PK198_16005"
     CDS             complement(5224..6072)
                     /locus_tag="PK198_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRF40296.1"
                     /translation="MKLSIGYSPCPNDTFIFDALAHGKIDTEGLEFDIVLADVEALNR
                     MAFEGQLDVTKLSYHAFAHLLERYVLLDAGSALGNNCGPLLIARRPLSDEEINSAKIA
                     IPGRFTTANFLLSLAWPDAADKVEMVFSDIEEAVLDGSVDAGLIIHENRFTYEQKGLC
                     KIADLGEFWEGRTGMPIPLGGIVIRRDLPPELQLRFNRVLARSVAYALDHPQQTLPYV
                     RAHAQEMDEAVMFQHIGLYVNEFTRNLGTKGRAAVQLMFDMARERGVLANRDSAIFLP
                     DGESND"
     gene            6084..>6588
                     /locus_tag="PK198_16010"
     CDS             6084..>6588
                     /locus_tag="PK198_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRF40297.1"
                     /translation="MNFQVLYEDNHLIAVNKPAGVLVQGDETGDTPLSELVKRYIKMQ
                     YDKPGEVFLGVIHRIDRPVSGVVVFARTSKALERMNKLFHDRTIDKTYWAVTAKRPDP
                     LKDHLVHYLVKDPAKNITKALDQPSNRNKDAKKAELDYELIMQVGDNCLLEVHPITGR
                     SHQIRAQL"
     assembly_gap    6589..6621
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6967..7605
                     /locus_tag="PK198_16015"
     CDS             6967..7605
                     /locus_tag="PK198_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40298.1"
                     /translation="MFIVEEEASKKPSSSAPAQAQPEKTATQAHTIHQSEQGQPGQVN
                     DKFMDILFQAMENNNPDGFDYMEFRQSINSLKKMPMDDKTRFQSAFAMAQTLGATPAG
                     LLDSAMHYIAVLKREEQQFEQALAAQKTKQIGSKEQEIGKLEQDIQAKAEQIKRLTAE
                     IEAHQQQLQQLRQDISEASVKVESTKNDFIASYNALVEQIQSDVENMKQYLK"
     gene            7602..7802
                     /locus_tag="PK198_16020"
     CDS             7602..7802
                     /locus_tag="PK198_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40299.1"
                     /translation="MSRISQKSFLLFALALLLLGTVWFVTSFPWGTIFFNTITLVIAV
                     LAVIVYFGVIVFILRRLFRKNS"
BASE COUNT         1791 a         2089 c         2098 g         1799 t
ORIGIN      
        1 ttagcagccg ttgcggacaa gcctttggta tgctcgaaga cgtattggta atactcgtca
       61 atgttgattt tttcgcccgg ccggtagggc gacaggaagt gctttcggat gccgaggctg
      121 ccgtcggggt ccatgcgcca ggagagttct atccaaaatt cgtcttcttc ccacacttcg
      181 ccgggattgg cctcgtaggt gaaggtgacg ggcttgccca tgcggcgggc ggcctcgcgc
      241 aatacgggtt gccggaaggc gatccagacg ccggcgtgcg tttcgtaagt attgaggtcg
      301 tggcgctcag aggccagacc catcggcagc acatagtcgg catagtaggc cgtctcgttc
      361 caggtgggcg tgagagcgat gtgcaggccg aatttttctt catcgcagag ggcttcaatc
      421 cacgaaaacc catcgggata cgtccacacc ggattgaaca cgcgggtgaa atacacatcc
      481 attttgcccc ggttttcttt gagcatgtgc ggcagcagga agctcatttc gaagaaagcc
      541 agcgggtact cgttggggaa gtgcagttcg ttccagaact tctgtgccgg cggggaatcg
      601 aagaatttcg ggtggtactt gttccaggta ttgggggccg taccgccttc ggtgcctacg
      661 ctgccggtga tgacattgag gaaatgcagg gcgcgggcta cggcccaacc gccgaggttg
      721 ccgctggctg cactgcgcca gttgtgagtt gacaaacgcg tgccggcctt gccgatgcct
      781 ttggccactt cgcggatcat ttcggccgtg acgccgcttt cctgctctgc aaattcaggc
      841 gtatattcgg cgtattcttc cacaacggaa gcgatgaact gctcgaaagt gcgcggtttg
      901 tcggggtgtt tggcggcgag atattcgtcc cagttcaccc agttttccat gtaatcgcgg
      961 ttgtacatgc cttcttccag caggattttg gcaatggcca acagcacggc ggcctcggta
     1021 ccgggatagc tcggcatcca gtagtcggcc atgctggcgg tattgctgag gcgtggatcg
     1081 aggacggcga gtttggcgcc tttcattttg ccctcaatga tgcgctgggc gtgcggattg
     1141 aagtagtggc cgctctccaa gtgtgcgctg atgaggagga tgaaatccgc gttggcatga
     1201 tcgggagacg ggcggtcgtg gccgtaccag agggcatagc cgaaacgggc gccggaggag
     1261 cagatattgg tgtggctatt gtggccgtcc acgttccagg cttgcaacac gcgatccatg
     1321 tagccttcgt ggccggggcg accgacgtgg gaggcgattt cgcggagggc tttgttgagc
     1381 ctgccggcaa tgtcgtcaag cacttgatcc caggtcacgc gctcccactt gccttcgccg
     1441 cgcttgccca cgcgcttgag cgggtacagg atgcggtcgg ggtcggtaag ttggttgacg
     1501 gtggccgggc ctttggcaca gttgcggccc cggctggcgg ggtggtaagg attgccctca
     1561 aacttctgca cctgcatggt ctccttgtca atatacgccg tgagaccaca tgccgactcg
     1621 cagttgaagc aagtggtggg cacaatggtg taattgcgct ttttgattct gggccaggac
     1681 tttgcgtcgt tttcggtcca gttgtcccat tgttccagtt cgctgcccgg cgcgaggcga
     1741 tcaacgggcg tttcgccttc cttgtggagg ttgggaatga tgcgcagttt ttctgcgatc
     1801 tgctctatta atgtgtgctt tttcatttta gatttttctt ttttggttat accaactgca
     1861 cccgttgcgg cgcttccacc cagatttttt cggtcacata catgccgatc attaccaaag
     1921 cagaagcggc ggcgctcatc atggcgtcct taccaagcag cagcagggcc agcggcaaca
     1981 cattgcctac cagcaccgcg ccgatccaga acatattgcg gtagcggcct ttgagaatca
     2041 tcttcaccgt cagtttggct tcctgtgtgg ggtgcgtagt cacgaactcc agcaccatca
     2101 gcgccatatt gaccaccaag ccgatgatga gcgtgttgcg cagatagtcg gtccacacga
     2161 tgccttcatt ggtaattaaa ttcacgatga ggaacaccgc cgcgccggcc ataaaactgt
     2221 gtaccaacat gtgcagcgcc agcgaaggac tttgccagaa atcgcggccc ttggcctgcg
     2281 caaacaggaa ggccgtataa accgccgtca tggtggctgc cgccaaagtg accgcataca
     2341 gcaccggagt gtagccgcct ttgaggtcga agaacatcag ggctgccagc agggccagca
     2401 ggccgccgaa cacagaaatg gtatatccgc cgcgcaccaa ccagctcttc cactgcgggc
     2461 gcaacaacac gttgaggaag cgatcaggac ggtctaagtc cttgaccaac aaggctcccg
     2521 tagccatcag aaaaaacagg cccaagccca gcgcccacca gaaagtcgta tcgctcacag
     2581 acgaaagccg gaacaaagaa cccatcaacg ccaccaaaac agcgcccgca gaaacggcct
     2641 tcgtgaccac atacgccgac acttcccagc cccagaggat gcctttcacc ggcgcatcat
     2701 atacgcggcg gggttggccc atgagcaggt caatggattt ggcgggcgtg cctttggcca
     2761 gatcgccggc agctacggca ctgccgtttt tgtcgagcag ggcattcact aagttgcctt
     2821 ccgaaaaggc caatttttcc gttttttgtg caaagtggcc cacgccgtgc gactgctcgt
     2881 tccagaaata atcgctctgg cgcgcggtgg ctaccggatt gagcgaagcc tcgtcgccat
     2941 tgatatagaa caagttcggc acagtgcctt tggcggcttt gcgcacgctc acctgctcgc
     3001 ggcccagcat cacggaaatc tcggaattcg ggtcgtccat atcgccggaa atgatggcgt
     3061 gttcggggca cacgttcacg caggccggtt cgaggcccac atctatgcgg tgcgcacagt
     3121 agttgcactt ggccgccgtg tgtgtttcgg ggtcaatgta cagcgcgtcg tagggacagg
     3181 cctgcataca ggatttgcac ccgatgcagc ggtttttgtc aaaatccacg atgccgtcat
     3241 cccgaaaata aagcgcctcc acagggcaaa tttctacgca aggcgcatcc gtacagtgat
     3301 tgcagcgcat caccgaaaag agccggcgcg tgtgtggaaa ctcgcctttt tctacctgtt
     3361 ttacccaggt tctaaagacg cccaccggca catcgtgctc tgatttacag gcagttgtac
     3421 aagcatggca gcctatacac ttccggttgt caatgataaa tccaaacttc atacactctg
     3481 gttttaataa aattggcggc taaatgtatg aagggttttt catatttata gacttgcgga
     3541 attttttttg ttgatttagt actccggggt tgattcaaat gtctgaaaaa gagattagac
     3601 gtattatttt atagtgaacc actccctgca caggctcaac gcatcgtgcg aatcacgcgt
     3661 tttttttgta taacctgctg aatgggaacc ccttccatgc gagattccag ccaggaaagg
     3721 atgtcgagat acatgaatgg ccgccgctcg atggggtcgt tcatcaacaa agacaacttg
     3781 gtatgcagcg tttcaaactc gcttctgagg ccctcggtcg gacggccgtg cacccggcga
     3841 atgaagcgca gaatctccaa ctgcaccgcc tgtaaattct cgatgtgcag caaaaagcga
     3901 tacaccgatt tggccaaatg ctccaccaat tctatatgcc ccagctcata atgcgcgatg
     3961 aggtgcagga tgcgcgcaaa acagtggatg tcgttgcggt agtcggtcgt tttttgttgt
     4021 atgattttgt tcaaaaaatc aatggcgccg ttttggtcgc cgttgccgaa acagagacag
     4081 gccaatcggt agtgaaattg caggatgcgg ttttggtccc aattgtaggg attgcgctca
     4141 atgagttcgt tcatttcggg gacgatgtga acgcccctct cgtaatcgcc ccgcaggtag
     4201 tgcaggttga tgaggtggat gtagcggtag agggtataaa gcccttccag atttttgttt
     4261 tgccccactc ccacacgctc tgggaaggtt tccaactgct cccaaacggc ctcaaatctc
     4321 acatcgtaga gactgttgaa caaagcgctg aggaggttgt gtatgccctt gaggtacatg
     4381 gccggatgca gtgccgacat ggccggcgag gcttcaaaca gttctgtcca tttttgcgca
     4441 tagcgatagc aggccggaaa ctcctgacaa atatggtgca gccaaacatg cgcctgacag
     4501 taataaatgc ggcccagaaa gtcgagttcc tgaaatgttt caaccggcat tcgctcccga
     4561 aaaaaagcct tgacacgctc gtggtcttcg cggttgcggg caaaaccgag ctgaaggtac
     4621 aggccgtaca tttcgagcga gagattggaa aaaccatgcg tgacggcaat ttcgcgggtc
     4681 agttcggcgc tctgccggct cagttcgcgg gcacggcctt caatgctgcg agtgatgtac
     4741 tgcccttcga tgtgcttttc aaactctatg atttccaatg caatagactt aaactgcccg
     4801 ccggttgccc ggtctttggc tttcgccaga atatccaagg ccggcctgta caagcccttg
     4861 tcgtacagca ccctggcata gtctatgttt tcgcgcaaag cgatgtcttc attgcggttc
     4921 ttggagagca ggcgcaggct ggtgagcaat tgccggtaaa ggtgggcttt gagattgggg
     4981 agttgctctt ttttgagggc cgggattttt ttcaaaacag cggcctcatc gtaatctctt
     5041 tttttctcca aaacgtcaaa caattgcaca aacaacagtc catcagcact ttgatggcgg
     5101 cgggcaaaaa ttctgaaatg ccgcttttcg gcctgcgtga ggctgcgaat gagctgcatt
     5161 aagtcgtctg ttttttgttt gggcatgtct aattggaaag gtaatcggcc aatactgcct
     5221 gcattaatcg ttggactcgc catcgggcaa aaatatcgct gaatcccggt tggccaaaac
     5281 gccccgctcc cgcgccatgt cgaacatcaa ttgcacggcg gcgcgtcctt ttgttccaag
     5341 atttcgggtg aactcattga catacaagcc aatatgttga aacatcaccg cctcgtccat
     5401 ttcctgtgca tgagcgcgca catacggcag cgtttgttgc gggtgatcga gtgcgtaggc
     5461 cacactgcgg gccagcaccc ggttgaaacg cagttgcagt tcgggcggca ggtctcgtcg
     5521 gatgacaatg cctcccaatg gaatgggcat tccagtgcgg ccttcccaaa actcgcccag
     5581 atcggcgatt ttacaaagcc ctttttgctc ataagtgaag cggttttcat ggataatcag
     5641 ccccgcatcc accgagccgt ccagcaccgc ctcctcaata tcggaaaaaa ccatttctac
     5701 cttgtcggcg gcatcgggcc aggccaagct gagcagaaaa ttggcggtcg taaacctccc
     5761 cggaatggct attttcgccg agttgatttc ctcgtccgac agcggtcggc gggcaatgag
     5821 cagcggaccg caattattgc ccaaagcact gcctgcatcc aacagtacat agcgctccaa
     5881 caagtgtgcg aaggcgtgat aactgagttt ggtcacatcc aactgccctt caaaagccat
     5941 gcggttgagc gcttccacat cggccagaac gatgtcgaat tccagccctt cggtgtcaat
     6001 tttaccgtgc gccaaagcgt caaaaataaa ggtgtcgttg ggacaaggcg aatagccgat
     6061 ggataatttc atacctttgt gcaatgaatt ttcaagtact ctacgaagac aaccacctca
     6121 tcgcagtcaa caagccggcg ggcgtgctgg ttcaaggcga tgaaaccggc gatacgcccc
     6181 tgagcgaatt ggtgaaacgc tacatcaaga tgcaatacga caaacccggc gaggtgtttc
     6241 tcggcgtcat tcaccgcatt gaccggcccg tgagcggcgt ggtggtcttt gcccgcacca
     6301 gcaaggcgct ggagcgcatg aacaagctct ttcacgaccg gaccattgac aaaacttatt
     6361 gggccgtgac cgccaaacgc cccgatccgc tgaaagatca tctggtgcat tatttggtaa
     6421 aagaccccgc caaaaacatc accaaagccc tcgaccagcc cagcaatcgc aacaaagatg
     6481 ccaaaaaagc ggagttggac tacgaattaa tcatgcaggt gggcgacaac tgcctgctgg
     6541 aggtgcatcc catcaccggc agatcgcacc agatcagggc gcaattggnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nttcgcggcg atgtgaaata cgggtttccg gcgctccatc
     6661 ccggaggctc cattttgctg cactgccgct cgctgagctt tgagcatcct gtaaaaaaag
     6721 agcaggtaac catcaccgca gaaccgcccg acgatcagat ttggaggttg ttcgagaagt
     6781 agaagcagca tttttgaagc caacggggcc gatctccttc ctctgctgta aaaaaatgct
     6841 cgtagagccg ccatcttatt tggtctatac tttgcggttg caggaaacta ttatctttgc
     6901 tgattcttat aatatttccg tacagaatcg ggacgcactg atcttatgct gaaaaatctc
     6961 aaatctttgt tcatcgtcga agaagaagct tcgaaaaagc cttcctcatc ggcgcctgca
     7021 caggcacagc ctgaaaaaac cgccacgcag gcacatacca tccatcagtc tgaacaaggt
     7081 cagccgggac aagtgaacga caagttcatg gacatccttt tccaggcgat ggagaacaac
     7141 aacccggacg gcttcgatta catggaattc cgccagagca tcaactctct caaaaagatg
     7201 cccatggacg ataaaacccg cttccagtcg gccttcgcta tggcgcaaac actgggcgcc
     7261 acacccgccg gcctgctcga ttcggccatg cactacatcg ccgtactgaa acgggaagag
     7321 cagcagtttg aacaggcttt ggcagctcaa aaaaccaagc agatcggcag caaagagcag
     7381 gaaatcggca aattggagca ggatattcag gccaaagccg aacaaatcaa gcgccttacg
     7441 gcagaaatcg aagcgcatca gcagcaattg cagcagctcc gtcaagacat ctccgaagcc
     7501 tccgtcaagg tggaaagcac caaaaatgac ttcatcgcgt cttacaacgc gctggtggag
     7561 cagattcaat ccgacgttga aaacatgaaa caatacttga aatgagccga atttctcaaa
     7621 aaagtttcct cctcttcgca cttgcactgc tgttgctggg taccgtgtgg ttcgtcacct
     7681 cgtttccctg gggcacgatt ttcttcaata caataacatt ggtgattgca gtattggcgg
     7741 tcatagtata tttcggcgtc atcgttttta tcctccgccg cttgtttcgc aaaaattcct
     7801 aattgatata
//
LOCUS       DAORLF010000214         7787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5068_length_7787_cov_2.133213, whole genome shotgun sequence.
ACCESSION   DAORLF010000214 DAORLF010000000
VERSION     DAORLF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5068_length_7787_cov_2.133213"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PK198_16025"
     CDS             <1..949
                     /locus_tag="PK198_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40300.1"
                     /translation="LPVRKDSERLAVGSEQLAAGGGQWAFGGGQLAVGGEQLAVGGGH
                     VLREVSDSPLTPHSSPLAPHPSLLIIEDNPDVVEYLRACLESEYQLEYAYNGRAGIEK
                     ALEIIPDIVISDVMMPEKNGFEVCETLKYDERTSHIPIVLLTAKATVEDRISGLQRGA
                     DAYMAKPFHRNELMAVLGNLIELRQKLQQRYASAQAALPPAPDAGVQMEDAFLLRFRH
                     IVEARLDDSSLTGEDVCRRLGMSYSVVYRKLSALTGRSLNVYIRLIRLQKAAELLSDL
                     SLSVSEIAYQTGFNDPKFFSRVFAEEFKATPTEFRKKTL"
     assembly_gap    1137..1193
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1194..>5119
                     /locus_tag="PK198_16030"
     CDS             <1194..>5119
                     /locus_tag="PK198_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRF40301.1"
                     /translation="IIQLLFFWLVSSLPVSAQFSGGAEALNESRVIHRAWHLTNLRGS
                     NYIVKVSGSSIVATDNDINDASTFIIEPGIDDANWLTADSRWFHIRNLSTGQYLTVAH
                     SGGLPRAVSLTEYNAQSHAQQFRLIATGVASQYKLRSRISAGSGTDLVLETSSSGLLQ
                     ADSPKLDPSSEQKFTFNLAMPGSGRRYILCSPGGRFMSDNGIKAEGTQVIQNKDVDEA
                     AIWMLEHVENSFYRIRNTLTQQFLTAPSESSAGDIPVMTASAQGNRSQWELDQTDAFF
                     RLKSRAFPNLFISATDQASGHPVRLRTSADSFRRWLVSAVEGELETVPGDYARIESGV
                     LPSGCLFSYGSEFKKALCERVGLNPAQSELYFNFIREAIAAKEGESRVDELLQKFDLN
                     NFGHRVDLALMVRYYITEVLPFVPFSQWSPAAQAAMGTFQSKITQIRSDYADRLFNAW
                     NAYVQENGSNLTMADIYFFWIDGDGFQWPVYYDVSETEAYLIADYVSGAATFGERNNG
                     IVGATAIGGTVATLAVVYAKAPFLILWLAYHTGAPLTATGLAGGTSVGAYAVMAGGPV
                     IAVTVFAASFIAMQATEVSQFQQFTNTINEELAWAQQYVDLAAGLSGNNLLTRIKLLN
                     DLDLVLGAPVQGGFVHNTNDNFYQPAFTLSCVPSVTLDMNSTGDATLTASQVVSGIIQ
                     LCGSEITYNLSQNQFDCNDIPSVQVTLMAQNDKGAQSCNVTVNLRDNTPPTVTCPAAQ
                     TLTLGANCTAVLPDYRYLATHLTDNCDIDMATQSPPAGTVISGAGDMTITFTVTDVSG
                     NSAQCSFTLTKVDNTPPTVVCPPTQTLELASDCSAAIPDYRSLATTYDACGIAGVVQV
                     APPGFVVSGVGSITAALVVTDVNNNVSQCNFTINKVDNTPPTIACPPTQTLVLGSACS
                     AALPNYTSLATANDNCGVQSVMQSPAAGTLVSGAGNMSVTLTVTDINGNTNTCTFTVT
                     KVDNTAPTIACPPTQTLVLGSTCSATLPDYRSLATANDNCGVQSVTQSPAAGTTVSNA
                     GNMTVTLTVTDINGNSNTCTFTVTKVDNTAPTVQCFNQTITFNGQTNIALNTDDLVDA
                     SDNCGVASISLSPDHITCEQLGQTVPVTITVTDVNNNVSTCTSYITVTGLPCGWSQNP
                     NGVGCAGGSSNTYNPNTGVWTATSTNCFYGPPYTSDATSFAQRTLCGDGSITAHVTSI
                     NGNGWAGVVMRESNAPVAKKAQLMTNLSQFHRREFRTTTNGAAQPQQIGSNNRHWLRI
                     VRAGNQFTMYASANGINWFPVGAQNIAMSNCIQMGLVASNFTANSTV"
     assembly_gap    5120..5141
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5142..5471
                     /locus_tag="PK198_16035"
     CDS             <5142..5471
                     /locus_tag="PK198_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40302.1"
                     /translation="VSFSGSNSGLGTGSIGQGAESIEPPQSFEVYPNPTGGELNVDLT
                     QYIGRSVRMEVYSIEGKLLQFREIDEVQTAVEHLDLSQYAGGMYFIKVQSRELPAATK
                     RVVMQRE"
     gene            5674..6417
                     /locus_tag="PK198_16040"
     CDS             5674..6417
                     /locus_tag="PK198_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40303.1"
                     /translation="MFIPYIYIVEDDPIIGADLTDRLQEMGHQAAGPFADGESAYEHL
                     SVRAPDLIIMDIQLEGAWDGIETARRIMAKCAAPIIFLTSNSDDATFKEAKATLPAAF
                     LSKPFRGRDLKYSIELALSNRAKETASEAPSAQPEEQAYILEDRLFVKIKDRWVRILL
                     QDIQWAEADDYACKVAVGDKEMFVGQTLGKFGEALAAKPEFMRVHRSFIVNLKYVDQI
                     GDMYLYIGRKQIPLSKTLKDELFNRIQKI"
     gene            complement(6524..6928)
                     /locus_tag="PK198_16045"
     CDS             complement(6524..6928)
                     /locus_tag="PK198_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40304.1"
                     /translation="MDHYLNTQNAYQRLLDEYLRYGSLVVAFDFDDTVYDYHQKGRSY
                     HDVIGLLRDLRRIGCYLICWTGQQDPEFVADYLRSNDIPFDAVNEHPPFRASQSRKVY
                     ANAYLDDRAGLAQVFGELSALVRQADQMSEAR"
     gene            complement(7105..>7330)
                     /locus_tag="PK198_16050"
     CDS             complement(7105..>7330)
                     /locus_tag="PK198_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40305.1"
                     /translation="DITMLQGMAIFAAGFALGLVGLISAIQQGKVCANGIAGIGAGYN
                     VFARTMILAVFPELYAIVAFAATFLISAGL"
     assembly_gap    7331..7340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7341..7567)
                     /locus_tag="PK198_16055"
     CDS             complement(<7341..7567)
                     /locus_tag="PK198_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40306.1"
                     /translation="MGTAIILAYVGLGLMLGLAGVGSAIGVSIGGNATIGALKKNEGA
                     FGSYMILSALPGTQGLYGFGGFFIINSSGVI"
     gene            complement(7574..>7787)
                     /locus_tag="PK198_16060"
     CDS             complement(7574..>7787)
                     /locus_tag="PK198_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40307.1"
                     /translation="MPVIGPILFVIFLIVGHGANLLISSLGSFVHPMRLTFVEFYKNA
                     GFEGGGKEYTPFASRKKQQDNHIQNA"
BASE COUNT         1850 a         2126 c         2062 g         1660 t
ORIGIN      
        1 gctaccggtg aggaaggata gtgagcggtt ggcggttggc agtgagcagt tggcagctgg
       61 cggtgggcag tgggcgtttg gcggtgggca gttggcggtt ggcggtgagc agttggcggt
      121 aggcggtggg cacgtcttgc gcgaagtgag cgactcgccc ctcactcctc actcctcacc
      181 cctcgcccct cacccctcac tcctgataat agaagacaac cccgacgtag tggagtacct
      241 gcgggcctgc cttgaatcgg agtaccaatt ggagtacgcc tacaacgggc gcgccggcat
      301 agaaaaagcg ctcgaaataa taccggacat cgtcatcagc gatgtgatga tgcccgaaaa
      361 aaatggtttt gaagtgtgcg aaacgttgaa atacgacgag cgcacgagcc atatccccat
      421 cgtgctgctc actgcaaaag ctaccgtaga ggaccgcatt tccgggctac agcgcggcgc
      481 cgatgcctac atggccaagc cgtttcatcg caatgagctg atggctgttt tgggaaatct
      541 gattgaattg cgccaaaaat tgcagcagcg atacgcctct gcgcaagccg cgttgcctcc
      601 tgccccggat gccggcgtac aaatggagga tgcctttctg ttgcgctttc gccatattgt
      661 ggaagccaga ctcgacgact ccagcctgac gggcgaggat gtatgtcgcc ggctcggcat
      721 gagctattcg gtggtgtacc gcaagttgtc ggcgctcacc gggcgctcgc tcaatgtata
      781 tatccgtctt atccggctgc aaaaagccgc cgaattattg tctgacctta gcctcagtgt
      841 atctgaaatt gcgtatcaaa ccggtttcaa cgaccccaaa ttcttcagcc gggtttttgc
      901 agaagaattt aaagctacac ctacggagtt tagaaaaaaa acactctaag taatgtaaat
      961 caacaagttg taaaacagcc ccgtcccgtc ggaacaaata gtccacctgt tggaacagat
     1021 attccacctt gcagaacaag tgttccgcct cgttgaacag atggtggacc ggacggaggc
     1081 gacatggcct gcatttttgt gaccgaaagc aatgcgatgt gttgcggcca atttttnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcatcc
     1201 aactgctgtt tttctggttg gtttccagct tgcctgtgtc tgcccagttc agtggtggcg
     1261 cagaagcatt aaatgagtct cgcgtcattc accgggcctg gcatctcacc aatttgcgcg
     1321 gttccaacta tatcgtcaaa gtatcgggca gctccatagt tgccacggat aatgacatca
     1381 acgatgccag tacattcatc atagaacccg gcattgacga cgccaactgg cttacggcag
     1441 actccagatg gtttcatatt cgcaacctga gtacagggca ataccttacc gttgcccaca
     1501 gcggcggctt gccaagagcc gtgagcctga cggagtacaa tgctcaaagc catgcccagc
     1561 agtttcgcct gattgccacc ggagttgcct cgcagtacaa gctgcgcagc cggatttctg
     1621 ccgggagcgg cactgatttg gttttggaaa cctcctcctc cggtttgctg caagcagaca
     1681 gcccaaagct ggacccatct tcagagcaaa aattcacctt caatttggcc atgcccggca
     1741 gtggtcgtcg gtacatcctt tgcagccccg gcggacggtt tatgagcgac aacgggatca
     1801 aagcagaagg cacgcaggtg atacaaaaca aagacgtaga tgaggctgcc atttggatgc
     1861 tggagcatgt agaaaattcg ttttaccgca tccgaaatac ccttacccag caattcctca
     1921 ctgcgccgtc ggagtcttct gcgggcgaca ttcccgtaat gacggcctca gcgcagggca
     1981 accgttccca atgggaactg gatcagacag atgctttttt tcgtttgaaa agcagagcct
     2041 ttcccaatct gtttatctcc gccaccgacc aggcatccgg gcatccggtc agattgagaa
     2101 catcggcaga tagtttccga agatggttgg tttcggcggt agagggcgag ctggaaactg
     2161 ttcccggcga ctatgcccgc attgaaagcg gcgtactgcc ctccggttgc cttttttctt
     2221 atggctctga atttaaaaaa gcgctttgcg agcgggtcgg attgaacccg gcgcaatccg
     2281 aattgtattt caatttcatc cgcgaagcga tagctgccaa agaaggcgag agcagagtgg
     2341 acgagttgtt gcaaaaattt gacctcaaca actttggaca tagagttgat ctggccctga
     2401 tggtgagata ctatattaca gaagtgttgc cgtttgtgcc tttttcgcag tggtcgcccg
     2461 cagcacaggc cgctatggga acattccagt cgaagataac tcaaatccgt tcagactatg
     2521 ccgaccgtct tttcaatgcc tggaatgctt atgtgcaaga aaacgggagc aaccttacta
     2581 tggcggacat ttattttttc tggattgatg gagacggatt tcagtggcca gtttattatg
     2641 acgtctcaga aacagaagct tatttgatag ccgactatgt atcaggcgcg gccactttcg
     2701 gggagcgcaa taacggcatt gttggcgcta ccgctattgg aggtaccgta gctaccctgg
     2761 cggttgtata tgcgaaagct ccttttttaa tactttggct tgcttaccat acgggcgctc
     2821 cgctgactgc taccgggctg gccggtggaa caagcgttgg ggcgtatgcc gtcatggccg
     2881 gggggccggt tattgccgtc accgtttttg cggcctcatt tattgccatg caggccactg
     2941 aggtgtcaca atttcaacag tttacaaaca ccatcaacga agaactggct tgggcgcaac
     3001 agtatgttga tctggcagcc ggtcttagcg ggaataattt attgacgcgc attaaattat
     3061 tgaacgactt ggacctggtg ctgggcgcgc ctgttcaggg cggttttgtg cataacacca
     3121 acgacaattt ctaccagccc gcattcacat tgagctgtgt acccagcgtg acgctcgaca
     3181 tgaacagcac cggcgatgcc acgcttactg caagccaggt tgtcagcggc attattcaat
     3241 tgtgcgggag cgaaatcacc tacaacctga gccaaaacca attcgactgc aatgacattc
     3301 ccagtgtgca agtcactctg atggctcaaa acgacaaggg cgcccagtct tgcaatgtta
     3361 ctgtgaattt gagagacaac acgccgccca ctgtcacctg cccggcagcg caaaccctga
     3421 cattgggcgc taattgcact gctgtcttgc ccgattatcg ctacctggca acgcacctga
     3481 ccgacaactg cgacatagat atggctacgc aatctcctcc cgcgggaacc gttatctccg
     3541 gcgccggcga tatgaccatt acttttaccg tgacggatgt cagcggcaac agcgcccaat
     3601 gcagctttac tcttaccaaa gtggacaaca ccccgcctac ggtcgtttgc ccacctactc
     3661 aaacgctcga attggcttcc gactgctccg ccgccatacc cgattacaga agtctcgcta
     3721 ctacttacga cgcttgcggc atcgccggcg tagtgcaagt ggcgcctccg ggattcgtcg
     3781 tctccggggt cggtagcatc accgccgcgc tggtcgtaac ggatgttaac aacaatgttt
     3841 cccaatgcaa cttcaccatc aacaaagtgg acaacacgcc gcccaccatc gcctgcccgc
     3901 ccacacaaac gctggtactg ggaagcgcct gctcggcagc cttgcccaac tacaccagcc
     3961 tcgctaccgc caacgacaac tgcggcgtgc aaagcgtgat gcaatcgccc gctgcgggca
     4021 cattggtctc cggcgccggc aacatgagcg tgacgctcac cgtgaccgac atcaacggca
     4081 acaccaatac ctgcaccttc acggtaacaa aagtggacaa taccgctccc accatcgcct
     4141 gcccgcccac gcaaacgctg gtgctgggaa gtacctgctc ggcaacctta ccagactacc
     4201 gcagcctcgc taccgccaat gacaactgcg gcgtgcaaag cgtcacacaa tccccggcag
     4261 cgggaacgac cgtctccaac gcaggaaata tgaccgtgac gctcaccgtg accgacatca
     4321 acggcaacag caatacctgc accttcacgg taacaaaagt ggacaacacc gctcccaccg
     4381 tgcagtgctt caaccagacc atcaccttca acggccaaac caacatcgcc ctcaacaccg
     4441 acgacctcgt ggatgcctcc gacaattgcg gcgtggcgag tatcagcctt tctcccgatc
     4501 acattacctg cgaacaattg ggccagacgg tacccgtcac cattaccgta acagatgtga
     4561 acaacaatgt atccacttgt accagctaca tcaccgtcac cggcctgcct tgcggctgga
     4621 gccaaaatcc caacggagta ggctgcgcag gcggcagcag caacacctat aacccaaaca
     4681 ccggcgtgtg gacggccacg agtaccaatt gcttctacgg gccgccctac acttcggatg
     4741 ctaccagctt tgcgcaacgc acgctctgtg gcgacggaag catcacggca catgtgacca
     4801 gcatcaatgg caatggctgg gccggtgtcg tcatgcgcga aagcaacgcc cccgtcgcca
     4861 aaaaagcgca gttgatgacc aacctgagtc agttccaccg ccgcgaattc cgcactacga
     4921 ccaacggcgc cgctcaacca caacaaatag gcagcaacaa ccgccattgg ctgcgcattg
     4981 tgcgcgcagg caatcagttt accatgtacg cctccgccaa tggaatcaac tggttccccg
     5041 tcggcgccca aaacattgcc atgagcaact gtatccaaat gggcttggta gcgagcaact
     5101 tcaccgccaa cagtactgtn nnnnnnnnnn nnnnnnnnnn ngtgagcttt tcaggtagca
     5161 acagcgggct gggaacaggc agcatagggc agggagcgga gagcatagag ccgccacaga
     5221 gctttgaggt ttatcccaat ccgacgggcg gagaactgaa cgtggatttg acgcagtaca
     5281 tcggtcgcag cgtgcggatg gaagtgtaca gcatagaggg caagttgttg cagttcaggg
     5341 aaatagacga ggtgcagacg gcggtggagc atctggattt gtcgcaatat gccggcggga
     5401 tgtatttcat caaagtgcaa tcgcgcgagc tgccggctgc aacgaagcgg gtggtgatgc
     5461 agcgggaata acagatcggc tatcgttggg ggcaacctgt tggggcaacc cttgtggttg
     5521 ccctcaaccg cccccccctc cgacgattct ggaaattccg aaattctgta aattctgata
     5581 agtattggag gatttatatc aaagccgcgg aggggaatgc cttcgcggct ttttttcaaa
     5641 atacacatca tcatttgttg ctaattttgc cccatgttca tcccttacat ttacatagtg
     5701 gaagacgacc cgatcatcgg ggccgatctg accgaccgtt tgcaggaaat ggggcatcaa
     5761 gcggcagggc cgtttgccga cggggagtct gcgtatgaac acttgtcggt tcgggcaccc
     5821 gatctcatca tcatggacat acaattggag ggcgcctggg acggcatcga aacggcccgc
     5881 agaatcatgg ccaagtgtgc ggcgcccatt attttcctca cctccaactc cgatgatgct
     5941 acctttaagg aagccaaagc gaccctgccg gcagcctttc tttccaaacc cttccggggc
     6001 cgcgacctga aatactccat agaactggcc ctcagcaacc gagcgaaaga gacagcgtcc
     6061 gaagctccct ctgcacagcc cgaagaacag gcttatatac tggaagacag gctgtttgta
     6121 aaaataaaag accgctgggt gcggatcctg ctgcaagaca ttcagtgggc cgaggccgat
     6181 gattacgcct gcaaagtagc agtaggcgac aaagaaatgt ttgtgggcca aacgctgggc
     6241 aaattcgggg aagcgctggc cgcaaagcct gagttcatgc gggtgcatcg ctccttcatt
     6301 gtcaatttga aatacgttga ccagatcgga gacatgtatt tgtacatcgg tcgcaaacaa
     6361 atacccctca gcaaaacttt aaaagacgag ttgtttaacc gcattcaaaa gatatgaaat
     6421 gcgggctttt cttatcgtca taatacccaa acaaatatga aaacgcctgt actcattgcg
     6481 ctgctgatgg gcctgtgttc cgtctcgatc attgcccagt gagctaccgc gcctccgaca
     6541 tctgatctgc ctgccgcacc aacgccgaca attcgccgaa cacctgagct aagcccgcgc
     6601 ggtcgtcgag ataagcattg gcatatacct tgcggctttg ggaggcccgg aagggcgggt
     6661 gttcattgac ggcatcgaag gggatgtcgt tggagcggag gtagtcggct acaaattcgg
     6721 ggtcttgttg gccggtccag cagatgagat agcagccaat gcgccgtaga tcgcgcaata
     6781 aaccaatgac atcgtggtag ctgcggccct tttggtggta atcatagacc gtgtcgtcga
     6841 aatcgaaggc caccacaagc gagccgtagc ggaggtattc gtcgagcagg cgctgataag
     6901 cgttttgagt attgaggtag tgatccattg ggtgtattgt atttttgatg tgtgcgcccg
     6961 cgccagggac agtagtggaa atggccgccc gacagcaact gccggcagcg gccattgtaa
     7021 cggatggccc gacggcctgc ctgtgccacc ctgccggttg atgggggcgg ggtggcaggg
     7081 tgatggccgg ggcgcccaaa aaaattacag ccccgcgctg atgaggaagg tagctgcgaa
     7141 ggccacgatg gcgtacagtt cggggaatac ggcgaggatc atggtgcggg caaacacgtt
     7201 gtagcccgcg ccgatgccgg cgatgccgtt ggcgcatact ttgccctgct gaatggcgga
     7261 aatgaggccg accaaaccga gtgcgaaacc agcggcgaaa atggccatgc cttgcaacat
     7321 ggtgatgtcg nnnnnnnnnn ttgatgactc cggaggagtt gatgataaaa aatccgccga
     7381 agccgtacaa gccctgggtg ccgggcaatg cgctcaaaat catgtaacta ccgaatgcgc
     7441 cttcattttt tttgagcgct ccgatggttg cgttgccgcc gatagatacg ccgatggcgc
     7501 tcccgactcc ggcaagcccc agcatcaaac ccaaaccgac gtaagctaaa ataattgctg
     7561 ttcccatatt gaattaagcg ttttgaatgt gattgtcttg ttgttttttt ctggatgcaa
     7621 aaggtgtgta ttccttgccg ccgccctcga agcctgcatt tttgtaaaac tctacaaagg
     7681 tgagacgcat ggggtgaacg aaggaaccga gcgaagaaat caagagattg gcgccgtgcc
     7741 ccacgatgag aaaaatgacg aacaaaatgg ggccgatgac cggcatg
//
LOCUS       DAORLF010000215         7774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5080_length_7774_cov_2.285659, whole genome shotgun sequence.
ACCESSION   DAORLF010000215 DAORLF010000000
VERSION     DAORLF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5080_length_7774_cov_2.285659"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..561
                     /locus_tag="PK198_16065"
     CDS             298..561
                     /locus_tag="PK198_16065"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF40308.1"
                     /translation="MDLFPTSQTTWSVYLLRCADGTLYTGCTSNLQARMERHGKGAVH
                     YSCTRLPVELITCVVFQDKYKAFDFEKYLKSGSGRAFAQKRLI"
     gene            635..>2547
                     /locus_tag="PK198_16070"
     CDS             635..>2547
                     /locus_tag="PK198_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40309.1"
                     /translation="MTELNARPMPTLSWNEIKTRAYTFAKEWEHESDEDAEAKSFWDG
                     FFEVFGISRRRVATFETPVKKADDKTGFIDLLWKGHILVEHKSRGKDLDKAVQQAKDY
                     FPGLKEHELPRYILVSDFQRFRLYDLDSGERHEFDLKDLPEKVNMFGFMAGYEKRSYK
                     EEDPVNIKAAELMGRLHDRLEGIGYTGHPLELYLVRLLFCLFADDTGIFEKGTFQQYL
                     DLHTKPDGSDLAAHLSLIFDALNTPPEKRLKTLDENLLAFPFVNGKLFEETLRPSAFD
                     GKMREMLLAACGLDWGSISPAIFGSMFQSVMNPQERRNLGAHYTSEKNIQKVIKPLFL
                     DDLYAEFEKAKGNTNRLRALHQKIASLHFLDPACGCGNFLIISYRELRQLELLILKEL
                     NKSGQGVLDVRELIKVDVDQFAGIEYDEFPALIAEVAMWLIDPKMNMKVSEAFGQYFV
                     RLPLSKAANIVHGNALRMDWREGFHRSTYDIHTENVNIIQVQEPPAQYHTVNVFTRNV
                     NLFSPENSPAQTMGSTGGGAFFDYILGNPPFVGKQLQNEQQKADMELVFKGVQGAGVL
                     DYVAAWYLKAAQYVQNTITRVAFVSTNSISQGEQTGILWSVLFNFYKIKIHFAHRTFS
                     WSNEARGNAAVHVV"
     assembly_gap    2548..2557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2586..>2908
                     /locus_tag="PK198_16075"
     CDS             2586..>2908
                     /locus_tag="PK198_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40310.1"
                     /translation="MDKYLFDYENIKGEPQMRKVKFINPYLIEAQNIFIRSLKKPLCP
                     VQEMTNGSMPNDDGNLLLSTKEKDSLLSMYPALAQHIRQFVGAIEFLNNKQRWCLWLK
                     DVHPN"
     assembly_gap    2909..3025
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3026..3573
                     /locus_tag="PK198_16080"
     CDS             <3026..3573
                     /locus_tag="PK198_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40311.1"
                     /translation="GEIRQPDCDYLIAPVVSSEKRAFIPIAIMSKEVIASNLVNIIPN
                     ATLYTFGVLSSEMMMTWIKSIGGRLKSDWRFTKDNVYNNFPFPLSPTDAQKKKVEEAA
                     QAVLDARARYPDSSLADLYDPASMPPDLVKAHQALDKAVDQCYRPKPFAGELSRIEFL
                     FGLYEQYTAGLFVEDAKKKKK"
     gene            complement(3765..4670)
                     /locus_tag="PK198_16085"
     CDS             complement(3765..4670)
                     /locus_tag="PK198_16085"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HRF40312.1"
                     /translation="MSTYRAKGKLLLTGEYVVLDGVPALALPTRLGQTLETSINVGDH
                     TLRWSSWDADGSLWLELELNPDTWQVLHSTDEAAAARLVQILRTAAHWNPAFRPLGQV
                     HTRLEFLRHWGLGSSSTLMANMAAWAGVDAFDLLAATWGGSGYDVACAQAGRPIVYQR
                     LHGRSQWVEIPFRPDFAEQLWFVYLGQKQDSREGIRRYRERSEQISSGLLDEIGALTL
                     AAASAVQLSDFEKIMAEHERIIGSLLGLEPVQQSRFSDHPGVVKSLGAWGGDFVLATG
                     PAEATKAYFAGKGLDTVLGYGELMG"
     gene            complement(4667..>4914)
                     /locus_tag="PK198_16090"
     CDS             complement(4667..>4914)
                     /locus_tag="PK198_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HRF40313.1"
                     /translation="AEELMMITAAVGLAQNFAAVRSLVTTGIQKGHMRMHLMNILNHI
                     GAAEAEVEAAAAHFADQTVSFSAVRDFIDQLRAVQPA"
     assembly_gap    4915..4966
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4967..6016)
                     /locus_tag="PK198_16095"
     CDS             complement(<4967..6016)
                     /locus_tag="PK198_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HRF40314.1"
                     /translation="MQDKQINGFSKKSKPGKLDWVTGVFCENPAEAQAEFSSFWHPDE
                     RLQKIIDGFSENTISNFVLPYGIAPNFLINGKTYAVPMVIEESSVVAAASSAAKYWMD
                     RGGFHVQVRDTVKLGQVHFLWSGEAARLRAVFPQMEQQLRTDAAYLTANMEKRGGGIT
                     GIELLDLSAQEPQLFQLKASFETCDSMGANFINSVLEEFASSLERFVENCAELPENER
                     RVEVVMAILSNYTPDCLVSAWVECPVDALGDPGKAEEGRDMAERFRRAVRIAQIDPWR
                     ATTHNKGIMNGIDAVVLATANDFRAVEACAHTYAARDGQYRSLSDCRIENGIFHFRLD
                     IPLALGSVGGLTKLHP"
     gene            complement(6091..7161)
                     /locus_tag="PK198_16100"
     CDS             complement(6091..7161)
                     /locus_tag="PK198_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HRF40315.1"
                     /translation="MAFQEQNGPERSQMSPVGDEDPTAASRKRDHIELAFRSKVEAAQ
                     LDARFYYEPLLAAHPQPGSLPEFPFLGKTMRAPLWVSSMTGGTDWAGTINPNLAKACR
                     EFGMGMGLGSCRSLLYSNETLPDFDVRDLIGEEQPLYANLGIAQLEQLIARDELQRAA
                     DMVAKLRADGLIIHVNPLQEWLQPEGDRFARPPIETITEVLERLPIKLIVKEVGQGMG
                     PESLRALMQLPLEAIDFAAGGGVNFALLELLRSDAEQLEAYLPLAQVGHTAAEMVEMT
                     NGIATELGDRMQCRQFIISGGIRDFLDGYHLIHTIRFPAVYGQASGFLRHARGDYEEL
                     RRYVEKVVRGLEVAHSFLVPKG"
     gene            complement(7214..>7774)
                     /locus_tag="PK198_16105"
     CDS             complement(7214..>7774)
                     /locus_tag="PK198_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRF40316.1"
                     /translation="TGVFIAAEGSGAHLNPAVSVALAITGKFPWADVPAYVAAQVLGA
                     MLGSTCVWLAYKKHYDAAEDAGHIQATFCTAPAIPHRFYNFLTEAIGTFALVFGVLFI
                     AAPDASLGALDALPVALLVLGLGLSLGGPTGYAINPARDLGPRIMHALLPIGRKAGSN
                     WSYSWTPIAGPLAGGALAAWVFGLLS"
BASE COUNT         1762 a         2136 c         2050 g         1647 t
ORIGIN      
        1 gcgagcttcg cctaaatcac agtcgcttcg gcgagcttcg gcggacgcag tccgcgtgtt
       61 tccaacgata tgttcgctcg ccgaagcttt agcgcaggcg agcttcgcct aaatcacagt
      121 cgcttcggcg agcttcggcg gacgcagtcc gcgtgtttcc aacgatatgt tcgctcgccg
      181 aagctttagc gcaggcgagc ttcgcctaaa tcacagtcgc ttcggcgagc ttcggcggac
      241 gcagtccgct ttgaacccta atcttattag acaaccaata tttaaaccct gactgcaatg
      301 gacttatttc caacttccca gactacatgg tccgtttatc tgctgagatg tgccgatggt
      361 acgctttaca caggctgtac ttccaatctg caagctcgta tggaacgcca tggcaaagga
      421 gcagtacatt acagctgcac ccgtttgccg gtggaactga tcacctgcgt tgtatttcaa
      481 gacaaataca aagcctttga ttttgaaaaa tacctcaaat ccggttccgg cagggctttt
      541 gcacaaaaaa gactgatcta agcgcccctc tttactacaa aaaccggctc atttcctctc
      601 cccgctccac tcatttgcca catttaccct atatttgacc gaacttaacg caaggcccat
      661 gcccacactg agttggaacg aaatcaaaac ccgcgcctat acttttgcca aagaatggga
      721 gcacgaaagc gacgaagacg ccgaagcaaa atccttttgg gatggcttct tcgaggtgtt
      781 cggcatttca cgccgcaggg tagccacctt cgagactcct gtaaagaaag ccgacgacaa
      841 aaccggcttt attgacttgc tttggaaagg ccatattttg gtagaacaca agtcgagagg
      901 caaagacttg gacaaggccg tacaacaggc caaagattac ttcccgggtc tcaaagaaca
      961 cgaactcccc aggtatatcc tcgtttctga tttccagcgc ttccggttgt acgatctgga
     1021 ttccggcgag cggcatgagt tcgatctcaa agacttgcct gaaaaggtca atatgttcgg
     1081 cttcatggcc ggatacgaaa agcgcagcta caaagaagaa gacccggtca atatcaaagc
     1141 cgccgaactg atggggcggc tgcacgaccg gctggaggga atcggctaca cggggcaccc
     1201 gctcgaacta tatctggtgc ggctgctctt ctgcctcttt gccgacgaca ccggaatatt
     1261 tgaaaaaggt actttccagc aatacctcga cctgcacacc aagcccgacg gcagcgacct
     1321 ggccgctcat ttgtcgttga tcttcgatgc gttgaacacc ccgccggaaa agcgactcaa
     1381 gaccctcgac gagaacctcc tggcgtttcc gtttgtaaac ggcaaactgt ttgaagagac
     1441 gctgagaccg tccgcatttg acggcaaaat gcgcgagatg ttattggcgg cctgtggctt
     1501 ggactggggc agtatttccc cggctatttt cggctccatg ttccaatcgg tcatgaaccc
     1561 tcaagaacgt cgcaacctcg gcgcgcacta cacctcggag aaaaacatcc aaaaggtcat
     1621 caagccgctc tttctggatg atctgtatgc cgaatttgaa aaagctaaag gcaataccaa
     1681 tcggctccgg gcgctgcacc aaaaaattgc atcgcttcac ttcctcgatc cggcctgcgg
     1741 ttgcggcaat ttcctcatca tctcttacag ggagctgcgg caattggaat tactgattct
     1801 caaggagttg aacaaaagcg gccagggcgt gctggatgta cgggaactca tcaaagtgga
     1861 tgtggatcag tttgcaggca tcgaatacga cgagttcccg gccctcattg ccgaagtggc
     1921 catgtggctg atagacccca aaatgaacat gaaggtgtcg gaggctttcg ggcagtattt
     1981 cgttcgcctg cccctctcca aagcggccaa cattgtgcat ggtaatgctc tgcgcatgga
     2041 ctggcgggag ggatttcata gaagcactta cgacatacac actgaaaatg tgaacattat
     2101 acaggtgcaa gagcctccgg cacagtacca caccgtgaac gtttttaccc gaaacgtcaa
     2161 cctgttcagc cctgaaaaca gtccggctca gacaatgggc agcaccggcg gaggcgcgtt
     2221 ttttgattat atattgggca atccgccgtt tgtgggcaag caactgcaaa atgaacaaca
     2281 aaaagcggat atggagctgg tttttaaagg ggtacaaggc gccggcgtgc ttgattatgt
     2341 ggcggcatgg tacctcaaag ctgcacagta cgtccaaaac accatcacca gagtagcttt
     2401 tgtgagtacc aacagcatat cacagggaga acaaaccggg attttgtgga gcgtactttt
     2461 taatttttac aaaatcaaaa ttcactttgc ccaccgcaca ttcagttgga gcaacgaggc
     2521 gagaggaaat gccgccgtac atgtggtnnn nnnnnnncat tatcgggttc ggcgcatacg
     2581 atgttgtgga taaatacctg tttgattatg agaacataaa gggagagccg caaatgagaa
     2641 aggttaaatt cattaatccg tatcttatcg aggctcaaaa tatttttata aggtctctaa
     2701 aaaagcccct ttgccctgtt caggaaatga caaatggaag tatgcctaac gatgatggta
     2761 atttactgct atctactaaa gaaaaggact ctttactctc tatgtacccc gcactcgcgc
     2821 aacatatcag acaatttgtg ggcgctattg agtttctcaa caataaacaa cgttggtgtt
     2881 tgtggctaaa agatgttcat ccgaatgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnntcggt gaaataagac aacctgactg cgattactta
     3061 atagctcccg ttgtttcttc cgagaaacgc gcattcatcc ccattgcgat aatgtctaaa
     3121 gaggtaatcg cttccaactt ggtaaatatc attcctaatg ctactctcta tacatttgga
     3181 gtcttgagtt cggaaatgat gatgacatgg attaagtcca ttggcggtcg tttaaaaagc
     3241 gattggcggt tcaccaaaga taacgtttac aacaacttcc ccttcccgct ctcccccacc
     3301 gatgcccaaa agaaaaaagt agaagaagcc gcgcaagccg tactcgatgc gcgcgcccga
     3361 taccccgaca gcagccttgc cgatttgtac gaccccgcct ccatgccgcc cgatctggta
     3421 aaagcgcatc aggcattgga caaagccgta gatcagtgct accgcccgaa accctttgca
     3481 ggagaattga gtcggattga gtttctcttt ggattgtatg agcaatatac tgcgggcttg
     3541 tttgtcgaag atgcgaagaa aaagaaaaaa taactacttg atagccaaag cattcagacc
     3601 tcactcctaa gaagtttcat tctggcgtgc cggggcacag gcttctcccc gaccctccag
     3661 aagaacaggc cgccccgtcg ggcgcctcag tcgctcctga cgtcgctacc ttcagccccc
     3721 tcacgcgacc aaacgcgacg aaaccaaccc tggccgttac gacctcaccc catcaactcc
     3781 ccatagccca gcaccgtgtc caatcctttg ccggcaaaat aagcctttgt tgcctcggcg
     3841 gggccggtag ccagtacaaa atcgccgccc caggcgccca gcgatttcac cacgcccgga
     3901 tgatccgaaa accggctttg ctgcaccggt tccaagccca gcaaagagcc gatgatgcgc
     3961 tcgtgttccg ccattatttt ttcaaaatca gacaattgta cagcagatgc agcagccaat
     4021 gtcagcgcgc cgatctcgtc gagcaagccg gaacttatct gctccgagcg ttcccggtag
     4081 cgccggatgc cctcccggct gtcctgtttt tgccccaggt aaacgaacca caactgctct
     4141 gcaaagtccg ggcggaacgg aatctccacc cactgcgaac gaccgtgcag gcgttggtaa
     4201 acaatgggcc ggcccgcctg cgcacaagcc acatcgtagc ccgaaccgcc ccaggtagcc
     4261 gccagcaaat cgaaagcatc caccccggcc catgccgcca tattggccat cagggtagaa
     4321 ctggagccaa gcccccaatg ccgcaaaaat tctaagcggg tatgcacttg ccccagcggg
     4381 cggaacgccg gattccaatg ggcagccgtg cgcaatattt gtaccagacg ggcggctgcg
     4441 gcctcgtcgg tgctgtgcaa aacctgccag gtatcggggt tcagttcaag ttccaaccac
     4501 aaactgccgt cggcatccca actgctccag cgcagcgtgt ggtcgcccac gttgatgctg
     4561 gtttccaaag tctgccctaa gcgagtgggc agcgccagcg ccggcacgcc gtcgagcacg
     4621 acgtattctc ccgtgaggag cagtttgccc ttggcgcgat aggtactcat gcgggctgca
     4681 cggctctgag ttggtctata aaatcgcgca cggcagagaa cgacacggtc tgatcggcga
     4741 aatgcgcggc ggcggcttcc acctccgctt cggcagcccc gatgtgattg agaatgttca
     4801 tcaggtgcat gcgcatgtgc cctttctgga tgccggtggt gaccaatgag cgcactgctg
     4861 caaaattttg cgccagcccc actgccgccg ttatcatcat cagttcctct gccgnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg tgcagtttgg
     4981 tcagaccgcc taccgaaccc aacgccagcg gtatgtccaa acgaaaatgg aaaatgccgt
     5041 tttcgatgcg gcaatcgctc aggctgcggt attggccgtc gcgggcggcg taggtgtggg
     5101 cgcaggcttc taccgcgcgg aagtcgttgg cggtggccag caccacggcg tctatgccgt
     5161 tcataatgcc tttgttgtgg gtggtagcgc gccagggatc aatctgtgca atgcgcacgg
     5221 cccggcggaa tcgctcggcc atgtcgcggc cttcttcggc cttgcccgga tcgcccagcg
     5281 catccacggg acattccacc caggcgctga ccaagcagtc cggcgtgtag ttggataaaa
     5341 tggccataac gacctccaca cggcgctcgt tttcaggcag ttcggcgcag ttttccacaa
     5401 agcgttccaa agaagaggcg aactcctcca gcaccgaatt gataaaattg gcgcccatcg
     5461 aatcgcaggt ttcaaagctc gctttgagtt ggaaaagctg cggttcctga gcgctgaggt
     5521 cgagcagttc gatgcccgtg atgccgccgc cccgtttctc catgttggcg gtgaggtagg
     5581 cggcatcggt gcgcaattgt tgctccattt gtggaaacac ggcgcgcaaa cgggctgcct
     5641 cgccggacca gaggaagtgc acctgcccga gttttacggt gtcgcgcacc tgcacatgaa
     5701 agccgccccg gtccatccag tatttcgcgg cgctcgatgc ggcagccacc accgagcttt
     5761 cttcgatgac catcggcacg gcgtaggttt tgccgttgat gagaaaattg ggagcgatgc
     5821 cgtagggcag cacaaaattg gaaatggtgt tttcgctgaa tccgtctatg attttctgga
     5881 ggcgttcgtc ggggtgccaa aagctgctga attcggcctg cgcttcggcg ggattttcgc
     5941 aaaatacgcc cgtgacccag tccaacttgc ccggttttga tttttttgaa aagccgttga
     6001 tttgtttgtc ctgcatgttt tttgaaagtc tgtctgttgt gaaaagctgc aagccattag
     6061 ctgcaagctg caagcctgat ggaagtaggt ttaccctttc ggaaccagaa acgaatgggc
     6121 cacttccaag ccgcgcacca ctttttccac atagcggcgc aattcctcgt agtcgccgcg
     6181 ggcatggcgc aaaaagccgg aagcctgacc atataccgcc ggaaaacgaa ttgtatgaat
     6241 gaggtggtag ccgtccaaaa aatcgcggat gccccccgaa atgatgaatt ggcggcactg
     6301 catccggtcg cccaattccg tggcgatgcc gttggtcatc tccaccattt cagcggcggt
     6361 atgccccacc tgagccaatg gcaggtaggc ttccaattgt tcggcatcgc tgcggagcaa
     6421 ttccaaaagg gcaaaattga ccccgccgcc ggcagcaaaa tctatggctt ccaatggcaa
     6481 ttgcatcagt gcgcgcaggc tttccggccc catgccctgt cccacttcct tgacaatcaa
     6541 cttgataggc agccgttcca acacctccgt gatggtttct atcggcggac gggcaaagcg
     6601 gtcgccttcg ggttgcagcc attcctgcaa aggattgacg tggatgatga ggccgtcggc
     6661 gcgcagtttg gccaccatat cggcggcgcg ttgcagttcg tcgcgggcga tgagttgttc
     6721 caactgtgcg atgccgaggt tggcatacag cggctgttcc tccccgatga ggtcgcgcac
     6781 gtcgaaatcg ggcagggttt cgttgctgta caagagcgag cggcaggaac cgaggcccat
     6841 gcccatgccg aattcgcggc aggctttcgc cagattgggg ttgatggtgc ccgcccagtc
     6901 ggtgccaccg gtcatgctcg acacccagag cggagcgcgc atggttttgc ccaaaaaggg
     6961 aaactccggc aggctgcccg gctgcgggtg tgcggccaac agtggttcgt aataaaagcg
     7021 ggcatcgagt tgcgcggcct ccactttgga gcggaacgcc aattcgatgt ggtcgcgttt
     7081 gcgcgatgcg gcggtagggt cctcgtctcc gacaggactc atttgcgacc gttcaggccc
     7141 gttctgctct tggaatgcca tgctgcaaag gtagtatttt cagggcattg caacgcagcg
     7201 cagtggaaat ggttcatgaa agcaacccga agacccaggc tgccaaagcg cccccggcga
     7261 gcggccctgc gatgggcgtc caactgtagc tccagttgct gcccgctttg cgcccgatgg
     7321 gcagcagtgc gtgcatgatg cgcggcccca ggtctctggc agggttgatg gcgtaacccg
     7381 tggggccgcc cagactgaga ccgaggccca acacgagcag ggcgacgggc agggcgtcga
     7441 gggcgcccag actggcatcg ggtgcggcga tgaacagtac gccgaaaacc agcgcaaagg
     7501 tgccgatggc ttcggtgagg aaattgtaaa accggtgcgg aatggcgggc gcggtgcaaa
     7561 aggtggcctg tatgtggccg gcgtcttcgg cagcgtcgta gtgttttttg taggccaacc
     7621 acacgcaagt ggagccgagc atggcgccca gcacttgcgc agccacataa gcaggcacat
     7681 cggcccaggg gaatttgccg gtgatggcca gggccacgct caccgcgggg ttgaggtgcg
     7741 cgccgctgcc ctcggcggca atgaacacgc cagt
//
LOCUS       DAORLF010000216         7737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5118_length_7737_cov_1.694220, whole genome shotgun sequence.
ACCESSION   DAORLF010000216 DAORLF010000000
VERSION     DAORLF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_7737_cov_1.694220"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>869)
                     /locus_tag="PK198_16110"
     CDS             complement(<1..>869)
                     /locus_tag="PK198_16110"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF40317.1"
                     /translation="ANTTFNIPNLPGGTYQVTILSANGCTATATATITAFPNPTCSIS
                     LVSDVIFGNDGALTVSATGGTAPYSFLWSNGATTQTITGLNGGVYSATVTDANGCTST
                     CSFNLIARSGLGDFVWEDIDKDGIQDANEPGVPNIKVRLKNAAGVVIDSTFTDAGGRY
                     SFVGLVPGTYSVQFVIQYPYLYTLLNRGGNDALDSDADSTTMLGMTPNVTLAPGEFNP
                     TLDAGIYVRPQIDDSDPCICLNNSTTETNGQFSEVITIFSYPGENWVLFNPMNLYLTS
                     SPPPPGIPILATGV"
     assembly_gap    870..879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<880..>1202)
                     /locus_tag="PK198_16115"
     CDS             complement(<880..>1202)
                     /locus_tag="PK198_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HRF40318.1"
                     /translation="VQILCNGANTGSATATATGGTPPYSFLWNNGQTTPAISNLAPGI
                     YNVTVTDLNGCDDIAGVQITQPPLLAVTITQVQGTCQGASNGVLMAIASGGTGAYTFL
                     WSGGG"
     assembly_gap    1203..1252
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1253..>1690)
                     /locus_tag="PK198_16120"
     CDS             complement(<1253..>1690)
                     /locus_tag="PK198_16120"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40319.1"
                     /translation="IGILVITDLTMEVTPRSVLCFGGNSGSALAVASGGTAPYTYVWS
                     NGVMGPELLNATAGTYSVTATEVNGCTVSRTITITQPTQLQATVTGVNAPCFGGTGSV
                     SVTAVGGTAPYTYLWNNGATTAQVTGITAGTYMVTVTDVNLCTA"
     assembly_gap    1691..1835
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1836..>2089)
                     /locus_tag="PK198_16125"
     CDS             complement(<1836..>2089)
                     /locus_tag="PK198_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40320.1"
                     /translation="CVGASTGTLTASATGGTAPYSYLWNTGATTPAIANLPAGTYSVT
                     VTDLEGCTATGSYTFFQAPVLEVTIAGSTVVCGQENGGAAT"
     assembly_gap    2090..2225
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2226..>2465)
                     /locus_tag="PK198_16130"
     CDS             complement(<2226..>2465)
                     /locus_tag="PK198_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40321.1"
                     /translation="GATTPTIAGLTTGTYVVTLTDLNGCRATASTSITVFDDLVATIA
                     KTNVDCFGASTGSATATATGGQAPYAFTWSNGAVGS"
     assembly_gap    2466..2603
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2604..4217)
                     /locus_tag="PK198_16135"
     CDS             complement(<2604..4217)
                     /locus_tag="PK198_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40322.1"
                     /translation="MTRKQAMLFILFSALPVFGALHAQITVQFNITNPPCFGVPTGAV
                     TAIASGGVAPYNYAWSNGAFGPTISTLPAGTYTVTVTDFVGNSKIQSATVTQPPLLGI
                     VFTGDTCTLPAFITASGVGGVGPYNYAWNTGHNGPSIVINSLGNYCLTLTDANGCGAI
                     GCVKITVPPINISLNSTNLLCPGVNNGQITSTVTGGQPPYNYQWSNGATTPNLINVGA
                     GVYTLTVTDARGCTRTATSTVTAPPPIVITLNTIRPSCTGFNNGSITASAAGGTAPYI
                     YLWNTGATGPTINNVGAGTYTVTVTDINNCPAQQTVTITPLSNLMLGAIGTAPNCPGF
                     NTGSAVANPSNGLPPYNFLWSNGQTTQTINNLAPGVYSVTVTDAAGCIRTASATVGTA
                     PAFNITVTSTNVTTCGAANGTVNVVVTQGVPPFTYIWNTGATTPNLTNLGAGTYSVTV
                     TSANNCVATGTATITAPPAIGATITATPLVCQGTAVGMATVNVVGGTAPFSFLWSNGA
                     TMQTISGLGAGTYSATVTDAFQCQAFATAT"
     gene            4275..4565
                     /locus_tag="PK198_16140"
     CDS             4275..4565
                     /locus_tag="PK198_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40323.1"
                     /translation="MAIQQYSCRNAMQDIYPLRKFAYAGGCRSKFVNKGDQQKGNSYR
                     IENARVTAVQNRLSVVQIPPTLLFSNKEKPFLSNIQSGGNFSCNKKVAPSVR"
     gene            4900..5799
                     /locus_tag="PK198_16145"
     CDS             4900..5799
                     /locus_tag="PK198_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40324.1"
                     /translation="MRYATLSFFLLAASLATAQIKPSNVYLFDVRVLNDSVYEFVQPK
                     FLTAFNADGYNNHPAFFSPTELYLSCQKPTESQPELYAFDLEKLTKTRVTNTPEGEYS
                     PFRMPDYYNFSAVRMEIARGDTVQRLWQFPMDRLSNGKPIFKYINKIGYYQWLNSRQV
                     ALFLVGQPNQLTVAEVDYDRLTPICTNPGRCFRMLPDGNLAYVEKSDFGSSRLMKRKP
                     MATNTEPEFMIETLPGSEDFAVLPDGSLLMGQGSRLYKYNPRTDDDWKQVADLRFYNI
                     RQITRIALSPGYRDFKIAIVGSE"
     gene            complement(5815..5934)
                     /locus_tag="PK198_16150"
     CDS             complement(5815..5934)
                     /locus_tag="PK198_16150"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRF40325.1"
                     /translation="MCPMHCPGSGSEQPGKCPKCEMDYIANKDHDHEGHDHQH"
     assembly_gap    5940..5995
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6216..6374
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6375..7041
                     /locus_tag="PK198_16155"
     CDS             <6375..7041
                     /locus_tag="PK198_16155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF40326.1"
                     /translation="TGNLPFFSIVCAYTLVGTEKKPTFMLEMASMKDLDGIFNKEIVP
                     HFDALYNFIFSLTRNEEDANDVMQDTLMRAYRALENYQQGTNAKAWLFTIAKNSYINE
                     YRRKAKAAIPTDFNAFTNVQDEDDSSYLSSYGDFRTEMFDKMMGDEVTDAINAIPDAF
                     RIPILLCDVEDFSYEEISAITGVKLNTVRTRIHRGRGLLRDLLMDYAAKRGFLPLPKD
                     SEE"
     gene            7077..7328
                     /locus_tag="PK198_16160"
     CDS             7077..7328
                     /locus_tag="PK198_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40327.1"
                     /translation="MHDPTLQALIGKINLFLDNGLSEAEESELLRQIQANPAYLQILS
                     REQSFREFIKTRIHRRKPSPVLIQSIKEKIKAAPGMNGL"
     gene            complement(7432..7683)
                     /locus_tag="PK198_16165"
     CDS             complement(7432..7683)
                     /locus_tag="PK198_16165"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRF40328.1"
                     /translation="MSISNGAEANILMEELTEMIARLDDSIRIPFLMHYQGFKYQEIA
                     DELNLPLGTVKSRIFFARKELKEMIGANYTSIPDLMDMD"
BASE COUNT         1728 a         1843 c         1797 g         1675 t
ORIGIN      
        1 accccggtgg caagaatagg aatccccggc ggcggcgggc tgctggtgag atacagattc
       61 atagggttga acagcaccca gttctctcca ggatagctaa aaatggtgat gacctcagaa
      121 aattggccgt tcgtctctgt tgtcgagtta ttgagacaga tacatgggtc ggagtcgtca
      181 atctgtggcc gcacataaat gcccgcatcc agcgtcggat taaattctcc gggcgccagc
      241 gttacgttgg gcgtcatgcc cagcatggtg gtagaatccg cgtcgctgtc caaggcgtca
      301 ttaccgcctc tgttcaataa agtatagagg taaggatact gaataacaaa ctgcacagag
      361 tatgtgcccg gaaccaagcc cacaaaggaa taacgcccgc cggcatctgt gaatgtgctg
      421 tcgatcacga cgccggcagc atttttcaat ctcactttga tattgggaac gcccggttca
      481 ttggcgtcct gaataccgtc tttatcaata tcttcccaaa cgaagtcgcc caatccgctg
      541 cgagcaatca ggttgaacga acaagtgctg gtacagccat tggcgtcggt caccgtagca
      601 gagtaaacac caccattgag gccggtgatg gtttgcgttg tagcgccgtt gctccacagg
      661 aaactgtatg gcgcggtacc gcccgtagcg ctcaccgtca gtgcgccgtc attgccaaag
      721 atcacatcac ttacgagcga aatagagcaa gtcggattgg ggaaagcagt aatggtagcc
      781 gtggccgtag ccgtacaacc attcgcactg agtattgtga cctgataagt accacccggc
      841 aggttcggga tattgaatgt tgtattcgcn nnnnnnnnnt gccgccgccg ctccacagga
      901 aggtgtaagc gcctgtaccg cccgatgcga tggccatcag tacgccgttg gaagcgccct
      961 ggcaagtgcc ctgcacctga gtgatcgtca cggccagtag cggcggttga gtaatttgaa
     1021 cgccggcaat atcatcacat ccgttcaaat cggtcacagt cacgttgtaa atacccggtg
     1081 ccaagttgct gatcgcgggc gtagtctggc cgttgttcca caagaagctg taaggagggg
     1141 tgccacctgt tgcggtggcc gtcgccgagc ctgtgttggc tccgttgcac agtatttgta
     1201 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggccgtac
     1261 agaggttgac gtctgttacc gtcaccatgt aagtgcccgc agtaattccg gttacctgcg
     1321 cggtagtagc gccattgttc cacaaataag tgtatggcgc agtgccgccg acagccgtta
     1381 ccgatacgct gccggtaccg ccaaagcacg gtgcattcac accggtgacc gtcgcttgta
     1441 gctgggtggg ctgtgtaatg gtgatggttc tggatacggt acaaccgttg acctcagtag
     1501 ccgttacgga ataggtacct gccgtggcat tcaacaattc aggtcccatc acgccattgc
     1561 tccacacata agtatatggc gcagttccgc ccgacgccac tgccagagca ctgccggagt
     1621 ttcctccaaa acacagcacg cttcggggcg tcacttccat tgtcaaatcg gttattacca
     1681 aaatacctat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtggc cgctcctcca ttttcctgac
     1861 cacaaactac ggttgagccg gcaatggtca cttccaatac gggcgcctga aagaaggtat
     1921 aagaccccgt agccgtacat ccttccagat cagttacggt aacagaataa gtgcctgccg
     1981 gcaaattggc aattgcaggc gtcgtggctc cggtgttcca cagataactg taaggagctg
     2041 tacctccagt ggctgaagcc gtgagcgtgc cagtagaggc gccgacacan nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnncgaac cgacagcgcc gttgctccat gtaaaggcgt aaggcgcctg gcctcctgtc
     2281 gcggttgccg ttgcacttcc cgtacttgct ccaaagcagt ccacattagt ttttgcaatc
     2341 gtggccacca agtcgtcaaa taccgtgatg gatgtactgg ccgtagctct acacccattg
     2401 aggtcggtca acgtaacgac ataggtgccc gtggtcaggc ccgcaatcgt gggtgtggta
     2461 gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnncgtcgct gtcgcaaatg cctggcactg aaacgcatcc
     2641 gttacggtag cggaataagt acccgcgccc aggccggaga tggtttgcat ggtggcgccg
     2701 ttgctccaca aaaaactgaa aggcgcagta ccgccaacga cgttcaccgt agccatacct
     2761 accgccgtac cctgacatac caatggcgtg gctgtgatgg tagccccgat ggccggcggc
     2821 gccgtgatgg tggcagtgcc tgtggctaca cagttgtttg cactggtaac cgtcacagag
     2881 taggtacctg cgcccagatt ggtcaggttg ggagttgtag cacctgtgtt ccagatgtat
     2941 gtaaacggag gaacgccctg agtgactact acattcacag tgccgttggc tgcgccacag
     3001 gtggttacgt tggtagacgt taccgtgatg ttgaatgccg gggccgttcc caccgttgca
     3061 gaagccgttc tgatacagcc cgctgcatct gtcaccgtta cggaatacac gcccggcgcc
     3121 aggttgttga tggtctgagt agtttggcca ttgctccaca ggaagttata cggaggtaag
     3181 ccgttcgagg gattggccac tgcactgccg gtattaaaac cggggcaatt gggcgcagtg
     3241 ccgatggctc ccaacatcaa gttggacagc ggcgtgatgg taacggtctg ctgtgccgga
     3301 cagttgttga tgtccgtcac ggtaacggtg taagtacccg cgcccacgtt gttaatcgtc
     3361 gggccggtag cgccggtgtt ccagaggtag atataaggcg cggtgccccc ggcagcgctc
     3421 gcagtgatgc tgccgttgtt gaagcccgta caggaaggcc gaatggtatt gagtgtgatc
     3481 acaataggcg gcggcgcggt gacggtagaa gtagccgttc gggtgcagcc tctggcgtcg
     3541 gtcacggtca gggtataaac tcctgcgccc acattgatga gattgggagt ggtcgcgccg
     3601 ttgctccatt gataattgta tggcggctgc cctcctgtta cggtcgaggt aatttgcccg
     3661 ttgttcacgc cgggacaaag caggttggtg ctgttcagcg aaatattgat gggaggcacc
     3721 gtgattttga cacacccgat ggccccgcag ccgttggcat cggtgagtgt aagacaataa
     3781 ttgcccaagc tgttgatgac gatgctgggg ccgttgtgtc ctgtgttcca ggcataatta
     3841 taaggcccca caccgccaac gcccgatgcc gtgataaagg ccggcaaggt gcaggtatcg
     3901 ccggtaaaaa cgatgcccaa aagcggcggc tgagtgaccg ttgcactctg gattttagaa
     3961 tttcccacaa agtctgttac ggtcactgtg taggtgcccg ccggcaaggt actgatcgtg
     4021 ggaccgaagg cgccattgct ccacgcgtag ttgtatggag ctacgcctcc tgaagcaatc
     4081 gccgtgacgg cgcccgtagg cacgccgaaa caaggcgggt tggtaatatt gaattgcacg
     4141 gtgatctggg catgtaatgc accgaataca ggcaaggctg agaataaaat aaacagcatc
     4201 gcctgtttgc gtgtcaagtg tagttttgga cacatagcgg attaataatt tgttagtgta
     4261 gtacattacc ctgaatggcc atacagcaat attcatgccg taatgccatg caggatattt
     4321 atcctttgcg taaattcgcc tatgcgggcg gatgtcgctc aaagtttgtc aacaagggag
     4381 atcaacagaa gggaaactca tacaggattg aaaatgcgcg tgttacggct gttcaaaatc
     4441 ggctttctgt ggtacaaata ccgcctacac tgcttttcag caacaaagaa aaaccttttt
     4501 tgtcaaacat ccaatctggg ggcaattttt cctgcaataa aaaagtcgca ccatccgtca
     4561 gatgatacga cctactacta acaaattata atcccatgaa tatgtaccaa tacaaagctc
     4621 tctcgtaaaa gctcaacata aaaacctgca tcagcagcaa attattatgt cgtttcaaaa
     4681 aaaacaactt cctttgcccg cgtaaagttc cttaaagaac gacttgctaa taacaaattg
     4741 taatcacatc cattgaactt tataatcctg tgaacatatt tttctaagcc cctccatgtt
     4801 ccctatacta catgaataat gctcttcgag agattcttct cgattgtctg gttcattttg
     4861 tgaggagttt acctaatttt acgcccaaac aatacagcca tgcgttacgc tacactatcg
     4921 ttttttctgc tcgccgcttc tttagctacg gctcaaatca agccttccaa cgtctatttg
     4981 ttcgatgtcc gggtattgaa cgactctgtt tacgagtttg tacaacccaa attcctcacg
     5041 gcttttaatg ccgacggcta caacaatcat ccggcttttt tcagcccgac ggagttgtac
     5101 ctgtcttgcc aaaagcccac tgaatcgcag cccgaactct atgctttcga cttggaaaag
     5161 ctcaccaaaa cccgcgtaac caacaccccc gaaggcgagt actcaccttt ccgaatgccg
     5221 gattattaca acttctcggc ggtacgtatg gaaattgcac gtggagacac cgtgcaacgc
     5281 ctatggcagt ttccgatgga ccgcctgagc aacggcaagc ctatcttcaa gtatatcaac
     5341 aaaataggtt actaccaatg gctcaacagc aggcaagtag cgctgttctt ggtgggccaa
     5401 cccaaccaac tgaccgtagc cgaagtggac tacgaccgcc tcacgcccat ctgcaccaat
     5461 cccggtcgct gcttccgcat gttgcccgac ggcaatctgg cttatgtgga aaaaagcgat
     5521 ttcggcagct cccggctgat gaagcgcaaa cctatggcaa ccaacacaga gccggaattc
     5581 atgattgaaa cactcccagg ctcggaagat ttcgcggtgc tgcccgacgg ttctctgctc
     5641 atggggcagg gcagccgcct gtataagtac aatccccgca ctgacgacga ctggaaacag
     5701 gtggccgatc tgcgttttta caacatccgt caaatcactc gcatcgccct cagccccggc
     5761 taccgggatt tcaaaatcgc catcgtaggc tcggagtagg cgcttgaatt ttcatcaatg
     5821 ctggtgatcg tggccctcgt ggtcgtgatc tttattggct atatagtcca tctcacactt
     5881 ggggcacttg ccgggctgct cgctgccgct gccggggcag tgcatggggc aaatgtaagn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngttgg
     6001 gcattgccat gatcgtgatg gtggccgtgg ccgcaggatg ttgcccagat ggatagcgaa
     6061 aacaggatgg ccatgagggc gaatttggaa aatttcatgt tgtaatggtt tttgatcggt
     6121 ttaaaaatgg aggggtaaag ataagttgtc ttggccggat cggcataact tgtgcgccgg
     6181 ctttcacagg agtcattttt gctccattat atttgnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnncaccgg gaaccttcct ttcttcagca tagtttgtgc ctatacgctt
     6421 gtcggaacag agaaaaagcc tacctttatg ctggaaatgg catctatgaa agatttagac
     6481 ggtatcttca acaaggagat agtgcctcat tttgatgcat tatacaactt catcttttct
     6541 cttacccgca acgaagaaga tgctaatgac gtcatgcagg ataccctcat gagagcttac
     6601 agggcattgg aaaactacca gcaaggcacc aacgccaaag cctggctctt caccatcgcc
     6661 aaaaactcct acatcaacga gtaccgccgc aaagccaaag cggccattcc taccgacttc
     6721 aatgccttca ccaatgtgca ggatgaagac gacagctcct atttgtcgag ctatggcgat
     6781 ttccgtaccg agatgtttga taaaatgatg ggcgacgagg taacggacgc catcaatgcc
     6841 attcccgacg ctttccgaat tcccattttg ttgtgcgatg tggaggactt ctcctacgaa
     6901 gagatttctg ccatcacggg cgtcaaactc aatacagtac gcacacgcat acaccggggg
     6961 cgcggtttgt tgcgcgatct gctcatggac tatgctgcca agaggggatt cttacctctg
     7021 ccaaaagatt cagaagaata aatcaacacg ttttttcacc gctaaaaatt actgttatgc
     7081 acgatccaac attgcaagca ctcatcggga aaatcaatct tttcctcgac aacggactga
     7141 gtgaagcgga agagtccgaa ttgctccggc aaatacaagc caaccccgca tacctccaaa
     7201 tacttagcag agagcaatcc tttagagagt ttataaagac aagaatacac agacgaaaac
     7261 catccccggt actcatccaa tccatcaagg aaaaaataaa ggccgctcct ggaatgaacg
     7321 gcctttagtg aaaatcatca ctatttgtcg aaatctattt ctcgttgctg caaatcataa
     7381 gcaaggagcg aatcagtatt tcatggttcg ctccttgctc cgaatgcagt attaatccat
     7441 atccatcaga tcgggaatgc tggtatagtt ggcgccgatc atctctttca actctttgcg
     7501 agcaaagaag atacggcttt tgaccgtacc cagcggcaaa ttcaactcat ctgcaatttc
     7561 ctgatatttg aacccctgat agtgcatcag gaagggaatg cgtatgctgt cgtccaaacg
     7621 ggcaatcatc tctgtcaact cctccatcag gatgttggct tccgcgccat tggaaataga
     7681 cacgctgccg gaattgaggt agcacagaca acctgtacta cctcaattcc gcgccat
//
LOCUS       DAORLF010000217         7735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5120_length_7735_cov_1.906901, whole genome shotgun sequence.
ACCESSION   DAORLF010000217 DAORLF010000000
VERSION     DAORLF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5120_length_7735_cov_1.906901"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PK198_16170"
     CDS             <1..1707
                     /locus_tag="PK198_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF40329.1"
                     /translation="IGTVHSLGVKLLQRFAFEAGVSPQVQIIADEDQQTLFNNSLAMV
                     LGPERVDAIDRLCRRLGMHSRQPHDWRKELRELTDIARSNAFSADTLRQSRQYSWDSF
                     AQYLPKASAETDEELLQALEQALDMTIAAIENNGDATKKTADALKEWKDARRQLHLDG
                     SLPWRFWAKIAKTEVAVKSRDDAQPLLDMTARHEANQTFQNDIRDYIFHIFDAAMDAL
                     QEYEQYKKRRGLIDYTDMETLILRLLDHPEVREILSEELDLLMVDEFQDTSPIQLEIF
                     LKLSRMAAHSVWVGDPKQSIYGFRGADPQLMQAIIEQSGGVRPEDIQRHSWRSRQDLV
                     WAANALFTKAFPEFPPDQIALDPKRRNTDDPAGMALALKHWHFRPEGETGRPPGKPWL
                     ENCIAQRIKDWLAEQPIVWDSHARIARPARPGDVAVICRTNDQCREVAEALHCAGLRA
                     AMARAGLLSTAEARLVTACLKFVLNYRDSLSVAEILLLAGGMDIEDIIEDRLQYLDLL
                     EQQRTEGLWSKDHPIIGRLLRLRKELAECSGVEILDVLLEELDLRRTIAAWAKPNSDW
                     TT"
     gene            1695..>2822
                     /locus_tag="PK198_16175"
     CDS             1695..>2822
                     /locus_tag="PK198_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF40330.1"
                     /translation="MDNIEALRRLALQYEDACTRLHSAASLGGLLLWLSDLENNEKDM
                     QATSPDADTVEVLTYHKSKGLEWPATICHSLENRLRAEVWGFDIVPQTEVVDLNNLLG
                     NRLLRYWVNPYGDQVQRTPLEERLKAGPEQAAITRRALREEARLLYVGLTRARDYLVF
                     PTVNRPPKWLNRVWHDGNEDYPVLDPTTSETLWEWEGTPLLADTEILAYPKDFTHAEL
                     PDDEVLYLENRQGKTAHPEAYIDPRREAATEGAGTHIVRPVYYGKEMVLPAEADRYTV
                     AKAIKSYLSALHTDYPAQEQELMAQYAIARYCPDEGVEAGQLLQCGRQWMSYLEQEFR
                     PRAMHRKYPVMLEREGRFFSAVLDTVLDTDAGWVIIQNSSFD"
     assembly_gap    2823..2838
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3104..3823
                     /locus_tag="PK198_16180"
     CDS             3104..3823
                     /locus_tag="PK198_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40331.1"
                     /translation="MKRILLFLFVMSAGMALSAQTADEIIQKYFENTGGIEKWKALKT
                     SKMEGKMAMQGMEFPGIITNKMPNKMRVDVNVQGMSIVQAYDGTDAWWINPFAGGTDA
                     QPMPDEMAESVTDQEFQSPFLDYAAKGHKVEYDGTTEVEGAKAHILKLTKKNGTVEYH
                     YFDAEHFVIIMSKSQIKNGEAKGQFTETYMSDYQEVDGLMFPFFMESKFDGQSVQKIT
                     VTSIKLNEEYKDDMFAMPKKK"
     gene            complement(3851..>4066)
                     /locus_tag="PK198_16185"
     CDS             complement(3851..>4066)
                     /locus_tag="PK198_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40332.1"
                     /translation="FLNHDSGVFFIDVLSFLESFKPQAVSFKLRTAGCPSALPTPRRG
                     HRDCASLVCSMLFALCQKPAESIVEQQ"
     assembly_gap    4067..4076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4093..>6613
                     /locus_tag="PK198_16190"
     CDS             4093..>6613
                     /locus_tag="PK198_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40333.1"
                     /translation="MSAWSLHAQSGSTLFGALRARHIGPATTSGRITAIDCINERPEV
                     MFIGTASGGVWKSNSAGAEFRPVFDDHTQSIGALAIDQSKPDIIWAGTGETNVRNSVS
                     VGDGIYKSTDGGITWKHLGLKNTERIADIIIHPTNSDIAYVAALGHLWNANEDRGVYK
                     TTDGGKTWSKILYIDENTGAADLSIDPRNPDVLYAAMWGFRRSPDFFDSGLGGKSGLY
                     KSTDGGQTWNTIHKGLPQETLGRMAVAAAPSNGDVVYLTVECKSQDKKGLYLSTDAGA
                     NWEKVSNDNSTTIRPFYFSHMVVDPQNDSILYKVGLNLAITENRGKGFRFMPGSVHSD
                     IHAVWVNPNNTKHVLIGSDGGVYESFDRGRSFKMFMNLPVGQFYHISVDDETPFNVYG
                     GLQDNGSWYAPSQKSGGISNADWKATFGGDGFWSFRHPTDKDIVFAEYQGGNLVRYNK
                     TTGMAQQIKPYATGDEAKYRFNWNAPIHLSPTNADRMYFGSQYLFMSTDKGDSWKRIS
                     PDLTSNDPKKQRQKESGGLTIDNSTAENHCTIYTIGESPVDGKMVWAGTDDGLLQVTT
                     DGGQNWSNVTANVPGLPKSTWVSHVEPGRFDRQTAFVTFDGHRSGDMKTYVYKTTDGG
                     KSWKSLATNQIEGYAFCIRQDLVNPNLLFLGTEFGLYISVDGGTNWARFENNLPKVAV
                     HDIAIHPRDHALGLGTHGRGAIIIDDITPLRQVTPEVLAKPLHFFSTAPTILRDPGAG
                     GGWFGGQGQFVGANPSSAAKVVYYMSKRHTFGKMYVEVWKDGELLRTLPAGKSAGINV
                     VEMPTSMEKPKSAPTNNRQALVGSLFGPNFPAGEYQVKLIKG"
     assembly_gap    6614..6626
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6627..7199
                     /locus_tag="PK198_16195"
     CDS             <6627..7199
                     /locus_tag="PK198_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40334.1"
                     /translation="FESTFKLANDPTSPHTAAERKVQYETTMRLYRLNERLAYLYDVL
                     ADVEKQAKAVSPKSKGKLKISVDKLAAKAGKEKDGLVFMGGDGYVNEDEALREELSDV
                     YRQVSSFPGKPTNTQLQLAAKMEKTIADLDQRVQTMLNTEVATLNTALTKAKLDAIKH
                     KTFEEFMKEETKTGDAASGFNGKWVLSQLK"
     assembly_gap    7270..7423
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7424..>7735
                     /locus_tag="PK198_16200"
     CDS             <7424..>7735
                     /locus_tag="PK198_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF40335.1"
                     /translation="RAILGESHAQSTVEGVSKEVFRWKMVTTWPPNFPIIGDTCARFA
                     EMVKAMSGGRLEIKVFGGGELVPALEAFDAVRNGVAEIGHGAAYYWAGKDPAASFFAT
                     VP"
BASE COUNT         1868 a         2262 c         1989 g         1423 t
ORIGIN      
        1 atcggcaccg tccacagcct cggcgtcaaa ctcctccagc gcttcgcctt cgaggccggc
       61 gtatccccgc aggtgcaaat catcgccgat gaagaccagc aaaccctctt caacaactcc
      121 ctcgccatgg tgctcggccc cgaacgcgtg gacgccattg accgcctctg ccgccgcctc
      181 ggtatgcaca gccgacaacc ccacgactgg cgcaaagaac tccgcgaact caccgacatc
      241 gcccgctcca acgccttctc cgccgatacc ctccgccaaa gccgccaata ctcctgggac
      301 agcttcgcgc agtacctgcc caaagcctcc gcagaaaccg atgaggaatt gcttcaggca
      361 ctcgaacaag ccctcgacat gaccatcgcc gccatcgaaa acaacggcga tgccaccaaa
      421 aaaacagccg acgccctcaa agaatggaaa gacgcccgcc ggcaactcca cctcgacggc
      481 agcctgccct ggcgcttctg ggccaaaatt gccaaaaccg aggtggccgt caaaagccgc
      541 gacgatgctc agcccctgct cgacatgacc gcccgccacg aagccaatca gacgttccaa
      601 aacgacatcc gcgactacat cttccatatt ttcgatgccg ccatggatgc cttgcaggaa
      661 tacgaacagt acaaaaaacg ccggggcctc attgattaca cagatatgga aacgctcatt
      721 ctcaggctct tagaccaccc tgaagtgcgc gagatcctca gcgaagaact cgacctgctc
      781 atggtggacg agtttcagga caccagccct atccaactcg aaatcttcct caaactctcc
      841 cgcatggccg ctcactccgt ctgggtgggc gatcccaagc agtccatcta tggctttcgc
      901 ggcgccgacc cccaactcat gcaggccatc atagagcaaa gcggcggcgt gcgccccgaa
      961 gacatacagc ggcactcctg gcgttcgcgg caagacctcg tctgggcagc caatgccttg
     1021 tttaccaaag ccttccctga attccccccc gaccagatag ccctcgatcc caaacgccgc
     1081 aacaccgacg accccgccgg catggctctc gccctgaaac actggcattt ccgccccgaa
     1141 ggcgaaactg gtcgcccgcc cggcaagccc tggctcgaaa actgcatcgc acaacgcatc
     1201 aaagactggc tggccgaaca gcccatcgtg tgggacagcc atgcccgcat cgcccggccc
     1261 gcccgccccg gcgacgtggc cgtcatttgc cgcaccaacg accaatgccg cgaggtagca
     1321 gaagccctgc actgcgccgg actgcgcgcc gccatggccc gcgccggact gctctctacc
     1381 gctgaggcgc gtttggtcac cgcctgcctc aagttcgtcc tcaactaccg cgactccctc
     1441 tccgtagccg aaatcctgct cttagcaggc ggcatggaca tcgaagacat catcgaagac
     1501 cgcctgcaat atctggattt actggagcaa cagcgtaccg agggcctctg gagcaaagac
     1561 catcccatca tagggcggct gctgaggctc cgcaaagaat tggccgagtg ctccggcgtg
     1621 gaaatcctcg acgtcctgct cgaagaattg gacctgcgcc gcaccatcgc cgcctgggca
     1681 aaacccaaca gcgattggac aacatagaag ccctgcgccg cctcgccctg caatacgaag
     1741 acgcctgcac ccgcctacac agcgccgcat ctttgggcgg ccttctgctc tggctcagcg
     1801 atttggaaaa caacgaaaaa gacatgcagg ccacctcccc cgatgccgac acggtagaag
     1861 tgctcactta ccacaaaagc aagggtttgg aatggcccgc caccatttgc cacagcttgg
     1921 aaaaccgcct gcgggctgag gtttggggct tcgacatcgt gccgcaaaca gaagtggttg
     1981 atctcaataa tttattgggc aacaggctct tgcgatattg ggtcaacccc tacggcgacc
     2041 aagtgcagcg caccccgctc gaagaaaggc tgaaggccgg ccccgaacaa gccgccatca
     2101 ctcgccgggc cttgcgcgaa gaagcccgcc tgctgtatgt gggtctcacc cgcgcccgcg
     2161 attatctggt gtttcccacc gtcaaccgcc cgcccaaatg gctcaatcgc gtgtggcacg
     2221 atggcaacga agactacccc gtactcgacc cgaccacctc cgaaacgctc tgggaatggg
     2281 aaggcacgcc gctactggca gataccgaaa tattggccta ccccaaagat tttacccacg
     2341 ccgaactgcc cgacgacgag gtgctttatc tcgaaaatag gcagggcaaa accgcccacc
     2401 cggaggcgta catagacccg cgacgagagg cggcaacaga gggcgcgggt acgcacatcg
     2461 tcaggccggt ttattatggc aaggaaatgg tgctgccggc agaagccgac cgatataccg
     2521 ttgccaaagc catcaaatcc tacctcagcg ctctgcacac cgactatccg gctcaggaac
     2581 aggagcttat ggcccaatat gccattgccc gctattgccc cgacgagggc gtggaggccg
     2641 ggcaattgct gcaatgcggc cggcagtgga tgtcgtattt agaacaagag ttcaggccgc
     2701 gcgccatgca ccgcaaatac ccggtgatgt tggagcgaga ggggcgcttt ttctctgccg
     2761 ttttagacac tgtattggac accgacgccg gctgggtcat cattcaaaac agcagtttcg
     2821 atnnnnnnnn nnnnnnnnat cacaacggcg gcaaatggga tgcccgcgct caaattctgg
     2881 cgccctggct actcctggca ggtgaggcgc ttgctgctgc aactggaaga ccacaggtgc
     2941 gaacaatggt acactttgta ttgtcatcag tactggtgga aattggctga aatttatcct
     3001 gcaactggcc cttttcatcg aaaaaacgga tagtttggaa taggccggcc atactttcgc
     3061 agacgtattt tctcatcatt caaaataaaa atccaagaca accatgaagc gcattctcct
     3121 ttttctgttc gtaatgagcg ccggtatggc ccttagtgct cagactgccg acgagatcat
     3181 ccaaaagtat tttgaaaaca caggcggcat cgaaaaatgg aaagctctga aaacgagcaa
     3241 aatggaaggt aaaatggcca tgcagggcat ggaatttccc ggtatcatta ccaacaaaat
     3301 gcccaacaaa atgcgcgtgg atgtgaacgt gcagggcatg tccatcgtac aggcttatga
     3361 cggtacggac gcctggtgga tcaatccgtt tgccggcggc accgacgcac agcccatgcc
     3421 cgatgaaatg gccgaatcag ttacagacca agagtttcaa agtcccttcc tcgactacgc
     3481 cgccaaaggc cacaaagtgg aatacgacgg cacgacagag gtggagggtg ccaaagccca
     3541 catcctgaaa ctgaccaaga aaaatggcac cgtagaatac cactactttg atgctgagca
     3601 ctttgtgatc atcatgagca agtctcagat caaaaacggc gaggccaaag ggcaattcac
     3661 ggaaacgtat atgagcgact atcaagaggt ggacggcctc atgttcccgt tctttatgga
     3721 aagcaagttt gacggtcaga gtgttcaaaa aataaccgta acctccatca aactcaatga
     3781 ggagtacaaa gacgatatgt ttgcgatgcc caagaagaaa taagatttcc cgatttgttt
     3841 tccgcagagt tcattgttgt tcaacaatgg actctgccgg tttttggcac agggcaaaga
     3901 gcatagagca gacaagcgaa gcgcagtccc gatgaccacg ccgaggcgtg gggagagcag
     3961 aggggcaacc cgcggtccgc agcttgaagc ttacagcttg tggcttaaag ctttctaaaa
     4021 aacttaatac atctataaaa aaaacaccac tgtcatgatt caaaaannnn nnnnnncggc
     4081 gcgcttatca ccttgagtgc gtggagctta catgcacaaa gcggcagcac gcttttcggt
     4141 gcattgcgtg cgcggcacat cggcccggcc accaccagcg gccgcatcac ggccattgat
     4201 tgcatcaacg agcggcccga agtcatgttc atcggtacgg ccagcggcgg cgtttggaaa
     4261 agcaacagcg ccggcgcaga gtttcgcccg gtttttgacg accacacaca atctatcggc
     4321 gctctggcca ttgaccaatc caagcccgac atcatctggg cgggaaccgg cgagaccaac
     4381 gtgcgcaaca gcgtttccgt tggcgacggc atctataaat ccaccgacgg cggcatcacc
     4441 tggaagcacc tgggattgaa aaataccgag cgcatagccg acatcatcat acaccccacc
     4501 aactcggaca tagcgtatgt agccgcactg ggccatctct ggaatgccaa cgaagaccgc
     4561 ggcgtatata aaaccaccga cggcggaaaa acctggagca aaatcctcta cattgacgaa
     4621 aacaccggcg ccgccgacct ctctatagat ccgcgcaacc ccgatgtgct ctacgcagcc
     4681 atgtggggct tccgccgatc cccggatttc ttcgattccg gcctcggtgg aaaaagcggt
     4741 ttgtacaaaa gcaccgacgg aggccaaaca tggaacacca ttcacaaagg tcttcctcaa
     4801 gaaacactgg gccgcatggc agtggccgca gctccctcca acggagacgt ggtttatctg
     4861 accgtagagt gtaaatcgca ggataagaag ggtttgtacc tgagtaccga tgccggcgcc
     4921 aactgggaaa aagtgagcaa cgacaacagc acgaccattc gcccgttcta tttttctcac
     4981 atggttgtgg atccccaaaa cgacagtatc ctgtacaaag tggggctgaa tctggccatt
     5041 accgagaacc ggggcaaggg tttccgtttc atgccgggca gcgtacactc cgacatacac
     5101 gccgtttggg tgaatcccaa caacaccaag cacgtcctca tcggcagcga cggcggggtg
     5161 tatgaatcct tcgaccgcgg gcgttctttc aagatgttta tgaacctgcc cgtgggccag
     5221 ttttatcaca tcagcgtgga tgacgagacg cctttcaatg tgtatggagg cttgcaggac
     5281 aacggttcct ggtatgcgcc ttcgcagaaa tccggcggca tcagcaatgc cgattggaaa
     5341 gccacttttg gcggcgacgg cttctggtct ttccgccatc ctaccgataa agacatcgtt
     5401 tttgctgaat atcagggcgg caacctcgtt cggtacaaca aaacaacggg catggctcag
     5461 caaatcaagc cttatgccac cggcgacgaa gccaaatacc gattcaactg gaacgcgccc
     5521 atccacctca gcccgaccaa cgcagaccgc atgtacttcg gctcgcagta cctgtttatg
     5581 tcaaccgaca aaggcgattc ctggaaacgc atctcgcccg atctgacgag caacgacccc
     5641 aaaaaacagc gccagaaaga atccggtggc ctcaccattg acaactctac tgctgaaaac
     5701 cactgcacca tttacaccat cggagagtcg ccggtggacg gcaaaatggt atgggcgggc
     5761 accgacgacg gcctcttgca ggtcactacc gacggcggtc aaaactggag caacgtgacc
     5821 gccaatgtgc cgggcttgcc caaaagcacc tgggtatcgc atgtggagcc gggacgattc
     5881 gaccgacaga ctgctttcgt cactttcgac gggcaccgca gcggcgacat gaagacttat
     5941 gtttacaaaa ccaccgatgg cggaaagtcc tggaagagtt tggctaccaa tcagatagaa
     6001 gggtacgctt tttgtatccg gcaagacttg gtcaatccca atttgctctt cctcggcact
     6061 gaattcggcc tttacatctc ggtggacggc ggcaccaact gggcgcgctt tgaaaacaac
     6121 ctgcccaaag tggctgtaca cgacatcgcc attcacccgc gcgatcacgc tctgggactc
     6181 ggcacgcacg gccgcggcgc catcatcata gacgacatca cgccgctgcg acaggtcacg
     6241 cccgaagtgt tggccaaacc cttgcatttc ttcagcactg cgcccaccat ccttcgcgat
     6301 cccggagcgg gtggcggctg gtttggcggt caggggcagt ttgtgggcgc taatccgagt
     6361 tcggcagcca aggtggttta ttacatgagc aagcgccaca ccttcggcaa aatgtatgtg
     6421 gaggtatgga aagacggcga attgctgcgc accctgccgg cgggcaagag cgcaggcatc
     6481 aatgtggtgg aaatgcccac ttcgatggag aagcccaagt cggcgcccac caacaacagg
     6541 caggcacttg tcggttctct gttcggcccc aatttccccg cgggcgagta ccaggtgaag
     6601 ctcatcaaag gcannnnnnn nnnnnntttg aaagtacttt taagctggcc aacgacccca
     6661 cttcaccgca cactgccgct gaacgcaaag tgcaatacga aacgacgatg aggctatacc
     6721 gcctcaatga gcgattggct tacctctacg atgtgctggc cgacgtggaa aaacaggcca
     6781 aagcggtttc gcccaagtcc aaaggcaagc tcaaaatttc ggtggacaag ctggcagcca
     6841 aggcaggcaa agaaaaagac ggcctcgtgt ttatgggcgg cgatggctat gtgaatgagg
     6901 atgaggccct gcgcgaagag ttgtcggatg tgtaccggca ggtgagttct ttccccggca
     6961 agcccaccaa tacgcagttg caactggcgg cgaagatgga aaaaaccatt gccgacctcg
     7021 atcagcgggt gcaaaccatg ctcaacacag aggtggcaac actgaatacg gcgctgacca
     7081 aagccaaact cgatgccatc aagcacaaga ctttcgagga atttatgaag gaagaaacaa
     7141 aaacaggcga tgctgcttct ggttttaacg ggaagtgggt actttcgcag ttgaagtaat
     7201 tttgtttcgg gtttcgggtt tcgggttgca tccgccgtag ccttggcgaa ggtgggttca
     7261 aggtttcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcgcca ttctcggcga
     7441 aagccatgcc cagtccaccg ttgagggtgt gagcaaagaa gtttttcgtt ggaaaatggt
     7501 gaccacctgg ccgcccaatt tccctatcat cggagacacc tgcgcccgtt ttgcagagat
     7561 ggtgaaagcc atgtcgggcg gtcgtttgga gatcaaagtg ttcggcggcg gcgagttggt
     7621 gccggccctg gaggcctttg atgcggtgcg caacggcgtg gccgaaatcg ggcatggcgc
     7681 tgcgtattat tgggccggca aagaccctgc cgcttcgttt tttgcaacgg tgccg
//
LOCUS       DAORLF010000218         7706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5143_length_7706_cov_1.947589, whole genome shotgun sequence.
ACCESSION   DAORLF010000218 DAORLF010000000
VERSION     DAORLF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5143_length_7706_cov_1.947589"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1294)
                     /locus_tag="PK198_16205"
     CDS             complement(239..1294)
                     /locus_tag="PK198_16205"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF40336.1"
                     /translation="MNYNALFQHPDFAQAKRIVFFGNQSSFDFRRGAYLFDLLIEEGR
                     LLRILAPEHGLFSEMQDQVGKDSHAYRGVECRSLYDSSKLNVVPDAGCFLGADALLVD
                     IPDVGARYFTYTTHLFWLLKSLTDNDIDLPVFLIDRPNPAGTKVEGTPLPERYASFVG
                     LPGLLHRHGMSTGQLADWMLGRLSAEIRLFKIQYVLPPGQMPINPSPNIPHHSTIQVY
                     PGQCFWEATTWSEGRGTTRPFELFGHPDLSGQSCTEMAAAFNARFAGQALLRPTTFIP
                     FFHKHEGRECRGFQLHLLDGAAYPTLFGALFLMRMARERMPEGAFWRPGAYEFDSECT
                     AAQILIGDDLLIGYVNG"
     gene            1369..>2448
                     /locus_tag="PK198_16210"
     CDS             1369..>2448
                     /locus_tag="PK198_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40337.1"
                     /translation="MTRLIVTIAAVLTTVALAVSCQSTGKNLSAAAPAADTLRYPQEK
                     HLKNLRQLTFGGNNAEAYFSFDSKKIVFQSDFTQWGVSCDQIFVADLAKSDMRNKPPR
                     MISTSHGRTTCSYFMPGNKTFVYASTHLGGAACPHVPPRGEKYVWPIYDTYDIFVADL
                     NGKITRQLTKEPGYDAEATVSPKGDKIVFTSTRSGDLELWTMNIDGSDLRQVTTGLGY
                     DGGAFFSPDGTKLVFRASRPKTEADIKEYKELLAQGLVQPTNMEIYVCNVDGSDLRQI
                     THLGKANWAPFFHPSSKKIIFSSNHHSPRGYNFNLFMIDLDGSNLQQITYDKTFDAFP
                     MFSFDGKKLIFSSNRHNGGSRDTNLF"
     assembly_gap    2449..2461
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2715..3194
                     /locus_tag="PK198_16215"
     CDS             2715..3194
                     /locus_tag="PK198_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HRF40338.1"
                     /translation="MMTNLNAIGLDVQKSQYLAEKLNELLANYSVFYQNTRGFHWNIR
                     GEKFFELHLKFEELYNDLLLKIDEVAERILTLGHSPKHNYTDYRKISQIAESVYISDG
                     AQAVKDILQSFKVIISLQRELLELSADAGDEGTNALMSDYIRAQEKLVWMYSAYSNH"
     gene            3260..4588
                     /locus_tag="PK198_16220"
     CDS             3260..4588
                     /locus_tag="PK198_16220"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRF40339.1"
                     /translation="MHKKVLLLLAAALCLASLATAQQELSTYLMPSVWQANRLNPAFF
                     PEHKLSVSLPGVYTNLLLTGLKYNEIVSKDANGNRVLDIDKGISMLDPDNLIRENTEI
                     ETLRLGLRLGKLSISAGHAVRFNAFLNYPKTLPQLIWQGNAQFIGQEVAFGPDLQLFG
                     FSETSLGIAFDVTKKLTIGGRAKFLSGLGDMSTSRNDLRLYTDEESYQLRLNADFQAD
                     ITGSIEYEGFSEPALNYEFGQFAFNQIFTSNTGMAWDFGAVWRGEKLELAASVLDLGA
                     INWKENARFYRLQGEYEYRGLDVAQSILNDSTNTGSVLDSLENIYNVTEGVAEYSTQL
                     SPRWYFSAGYQLTKKLRLGGVFYSEQYRGQSFPALAFSATAQVVPWWSVGGSYGWRNQ
                     RFDNIGAHTALKLGPVQFVAATDNLVSLLLQKSSNSANMRLGLNLLLGKR"
     assembly_gap    4611..4686
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4687..6418
                     /locus_tag="PK198_16225"
     CDS             <4687..6418
                     /locus_tag="PK198_16225"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRF40340.1"
                     /translation="LTMKIFTTTLSLLFAVILGAQTPAERLLHQLFEHDQAFLKKEFY
                     ELETPFPAVAEADELRRADFYRQELALLQAIDPTLLERNDRVSYELFGFILQERIAET
                     EYGAYLVPLNAEGGWYTDFALEADGWRPKTADDYRRLLRRMAAFRNYAAENIRLMETG
                     LKRGRTAPYDILRGKETIVDAFITERPDDSPFFKPFRQMPAAIPADLQDSIRTAARVA
                     ISQSLAPAYREFRKFWTEKYLPGAVRAPGIAAQPRGRAYYEERVRYFTTLNMTPEEVF
                     ETGQREVARIRAEMEATLRELKYAGSFADFLRFLRTDPQFYPASPRALLMEGSYYAKK
                     IDGKLPQYFGKLPRNPYGVSPVPDILAPTYTAGRYSPGNPKAQRAGFYWLNTYNLPAR
                     PLYALPALTLHEAVPGHHLQISLAQEMENVPEFRRNAYLSAFGEGWALYGEWLGKEMG
                     IYETPYEHFGRLTYEMWRACRLVVDPGIHVMGWDREKAIQFMTENTALSLHECTTEID
                     RYIGWPGQAVSYKIGELKIRDLRAKAEKELGDKFDIRAFHDLVLSEGTVPLYVLENLV
                     DEWIAQIKKK"
     gene            6641..>6920
                     /locus_tag="PK198_16230"
     CDS             6641..>6920
                     /locus_tag="PK198_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40341.1"
                     /translation="MMKRALLFAFYSFISLAWAQAQTCTPAQNFPDTAIAIPPSWSPL
                     RLMAGIQDTACVGQYFEFTLSIKAPAAIPGLPGVTVNSIAIPAANGVAN"
     assembly_gap    6921..6960
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6961..>7284
                     /locus_tag="PK198_16235"
     CDS             <6961..>7284
                     /locus_tag="PK198_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40342.1"
                     /translation="PRDTFACIKMFGIPNTPADVGQKDLTLSLTINTSFGQLSNIPYP
                     NAQLDPGGHYYLHVKPTGSSNCFVSSDREADLPVTGLYSRPNPTSGYTQIQANVLQAG
                     QYEFRV"
     assembly_gap    7285..7332
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1804 a         2214 c         1848 g         1663 t
ORIGIN      
        1 ctgatttcgg attcctgatt tcggattcct gatttcggat tcctgatttc ggatttgcga
       61 cgctgaaaga cgtaggtttt atgatcgttg tgccctctcc gagcaacaaa aaccatggaa
      121 atcataaaga tcagtgtcaa aaacaaagcg cagccttcag attccgggtt gccctacgct
      181 tgaagataaa ctgcgcaccg cgcaaagcgc gggaaagcga tgaactgccg aaggcagatt
      241 acccattcac atacccgatg agcagatcat ccccgataag gatttgagca gcagtgcatt
      301 ccgagtcgaa ctcatacgcg ccgggccgcc aaaatgcccc ctccggcatc cgttcgcggg
      361 ccattcgcat aaggaacagc gcgccgaaaa gggtaggata agcagccccg tcgagcagat
      421 gaagttggaa gccccggcac tcgcggcctt cgtgtttgtg aaaaaaggga atgaacgtcg
      481 tagggcgcaa taatgcctgc cccgcaaacc tggcattgaa cgccgccgcc atctccgtgc
      541 agctttgccc gctcaaatcg ggatgaccga acagctcgaa cgggcgggta gtgccgcgcc
      601 cctcgctcca ggtggtcgcc tcccaaaaac actgccccgg atacacttga atggtgctgt
      661 ggtgcgggat attgggcgat ggattgatgg gcatttgccc cggcggcaag acgtattgaa
      721 ttttgaaaag tcgaatctct gccgacaagc gaccgagcat ccaatcggcc aattgccccg
      781 tactcatgcc gtgccggtgc agcagtccgg gcaatcctac aaatgaggca tagcgctccg
      841 gcaaaggcgt gccttccact ttggttccgg cgggattggg gcggtctatc agaaaaacag
      901 gcaaatctat atcattgtct gtcagggatt tgagcagcca aaaaagatgg gtagtgtagg
      961 taaaataccg cgctcccacg tcgggaatat ccaccagcaa agcatcggca ccgaggaaac
     1021 aaccggcatc gggcactaca ttcagtttgg aactgtcgta cagggagcgg cactccaccc
     1081 cccggtaggc atggctgtct ttgcccacct ggtcctgcat ttcagaaaac aggccgtgtt
     1141 cgggcgccaa aatccgcaac aagcggccct cctcgatgag cagatcgaag agataagcgc
     1201 cccgccggaa atcgaagctg ctttgatttc caaagaaaac aatgcgtttc gcttgcgcaa
     1261 aatcaggatg ctgaaatagt gcgttgtagt tcatattcca aaaatcttac tcccaaatga
     1321 caatgaggcg atacagccgt tttatttttg ccgcaaaaat acaaaacgat gacacgcctg
     1381 attgttacca ttgctgccgt tctgacgacg gttgccctcg ccgtttcctg ccaaagtacc
     1441 ggcaaaaacc tctccgccgc cgcgcctgct gccgatacgc tgcgctaccc gcaggagaaa
     1501 cacctcaaaa acctgcgcca actcaccttc ggcggcaaca atgccgaagc ctactttagt
     1561 tttgacagca aaaagatcgt tttccagtcg gacttcaccc aatggggcgt ttcctgcgat
     1621 cagatttttg tggccgactt agccaaaagc gacatgcgga acaagccgcc gcgcatgatc
     1681 agcacctcgc acggacgcac cacttgctcc tattttatgc ccggcaacaa aacttttgtg
     1741 tatgcctcca cgcacctcgg cggcgcagcc tgcccgcatg ttccgccgcg cggcgagaag
     1801 tacgtctggc ccatttacga tacctacgat atttttgtgg ccgatctcaa cggcaaaatc
     1861 acccgccaac tgaccaaaga acccggctac gacgctgaag ccaccgtttc gcccaaaggc
     1921 gacaaaatcg tgttcacatc tacccgctcc ggcgacctcg aactgtggac gatgaacatt
     1981 gacggctccg acttgcgcca agtgaccacc ggcctcggct acgacggcgg cgctttcttc
     2041 tcgcccgacg gaaccaaatt ggtattccgt gcatcacgcc ccaaaaccga agcggacatc
     2101 aaggagtaca aagaactgct ggctcagggc ttggtgcaac ccaccaacat ggaaatttat
     2161 gtgtgcaatg tggacggctc cgacctgcgg caaatcaccc acctcggcaa agccaactgg
     2221 gcgccgtttt tccacccttc cagcaaaaag atcattttca gttccaacca ccactcgccg
     2281 cgaggctaca atttcaatct ttttatgatt gatctcgacg gcagtaactt gcagcaaatt
     2341 acctacgaca agacttttga cgccttcccg atgttttctt tcgatggtaa aaagctgatt
     2401 ttcagttcca accggcacaa cggcggctcc cgcgatacca acctgtttnn nnnnnnnnnn
     2461 nttatcgccg actgggtgga ttgaggctta tgggccacga tctgtgagtt ctgctcaatt
     2521 gagggcgtgt atgacagatt gcacaaaatc aggcatagtt ttcagacagt acacaatttg
     2581 acaaatcttc gagatttgtc aaattttcgc tccgccatac ggtgaatcct gtctaaagat
     2641 aatgcacacc aagtgaggaa gatcaccggc tgtatggccg gaataggcta tttttgctaa
     2701 aaatcaacaa catcatgatg acgaatctga acgctattgg tctcgatgtg caaaaatcgc
     2761 agtacctggc agaaaagctc aatgaattgc tggccaacta ctccgttttt taccaaaata
     2821 ccagaggttt ccactggaac atccggggag aaaagttttt cgagctgcat ctcaaatttg
     2881 aagaactgta caacgacttg ctgctgaaaa tagacgaagt agccgaacgc atcctgacgc
     2941 tgggccactc accgaagcac aattacaccg attacagaaa aatttcgcaa attgccgaaa
     3001 gtgtgtatat ctccgacggc gctcaggccg ttaaagacat tcttcagtca ttcaaggtca
     3061 tcatcagcct gcaacgcgaa ctgctcgaac tctcggcaga cgcaggcgat gagggcacca
     3121 atgcgctgat gagcgattat atccgcgccc aggaaaaatt ggtgtggatg tactcggcat
     3181 actcgaatca ttaactcaaa ctccggcctt ccctctttga acaggaaggc cggagactta
     3241 cacccaaaag cgattgaaca tgcacaaaaa agtactcctt cttctcgcag cagcgctatg
     3301 ccttgcctct ttggccaccg cgcaacaaga actcagcacc tacctcatgc cctccgtctg
     3361 gcaggccaac cgcctcaatc cagctttttt tccagagcat aaactgagcg tttcattgcc
     3421 cggcgtttat accaacctac tccttaccgg cctgaaatac aacgaaatag tgagcaaaga
     3481 cgccaacgga aaccgcgttt tggatattga caaaggcatc tccatgctcg accccgacaa
     3541 cctcatccgc gaaaataccg agattgaaac cctgcgcctc ggcctccgtt tgggcaagct
     3601 cagtatcagt gcgggccatg ccgtgcggtt caatgccttc ctcaactacc ccaaaacgct
     3661 gccccaactg atctggcagg gcaacgcgca gttcatcggt caggaggtcg ctttcggccc
     3721 cgatctgcaa ctgttcgggt tcagcgagac ctccctcggc attgcttttg atgttaccaa
     3781 aaaactgacc atcggcgggc gcgccaaatt tctcagcggc ctcggcgata tgtccacctc
     3841 ccgcaacgat ctgcgccttt ataccgacga ggaatcttat caactccgcc tgaacgctga
     3901 ttttcaggcc gatatcaccg gcagcattga gtatgaaggt ttcagcgagc cggcgctcaa
     3961 ctacgaattc ggacagtttg ccttcaacca gattttcacc tccaacaccg gcatggcctg
     4021 ggatttcggg gcagtctggc gtggcgaaaa actcgaactc gccgcatccg tgctcgatct
     4081 gggcgccatc aactggaaag aaaatgcccg tttttacagg cttcagggcg agtacgaata
     4141 tcgcggctta gacgtggcgc aaagcatcct caacgactcc accaacaccg gctcggtact
     4201 cgattcttta gaaaatattt acaacgtaac cgagggggta gccgagtaca gcacacagct
     4261 ttctccgcga tggtatttca gcgccgggta tcaactgacc aaaaaactgc gcctgggcgg
     4321 tgtgttttat tccgaacaat atcgggggca atcctttcct gcactggcct ttagcgctac
     4381 ggcgcaggtt gttccctggt ggagtgtggg cggatcttac ggctggcgca atcagcgttt
     4441 cgacaatatc ggcgcacaca cggcgctcaa actggggccg gtgcaattcg ttgccgccac
     4501 cgataatttg gtcagtctgc tcctccaaaa aagcagcaac agcgccaata tgcgcctggg
     4561 gctgaacctc ttgctgggca agcgatagcc tatcgtcggg gcaaccctcg nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnattg actatgaaga ttttcactac caccctctcc ctcctttttg cagtcattct
     4741 cggagcgcaa acgcccgccg aacgccttct gcatcaactc tttgagcacg atcaggcatt
     4801 tctgaaaaag gaattttacg aattggaaac accctttccc gccgtcgctg aggccgatga
     4861 actgcgccgc gctgatttct acaggcagga actggcgcta ctccaggcta ttgatcctac
     4921 cctactcgaa cgcaacgacc gcgtcagtta cgagttgttc ggcttcatcc ttcaggagcg
     4981 catcgctgaa acagagtacg gcgcctacct tgtgccgctg aacgcagagg gcggctggta
     5041 caccgatttt gcccttgagg ccgacggctg gcgccccaaa accgccgatg actaccgacg
     5101 actgttgcgc cgaatggctg cttttcgcaa ttatgcagcc gagaacatcc gcctgatgga
     5161 aaccgggctg aaacgcgggc gcaccgcccc ttacgacatc ttgcgaggca aggaaaccat
     5221 cgtggatgct ttcatcaccg agcggcccga cgacagcccg tttttcaagc ctttccggca
     5281 aatgcccgcc gccattcccg ctgatttaca ggattctatc cgcactgccg cccgtgtggc
     5341 catttcccag agcctggcgc ctgcctaccg tgaatttcgg aagttctgga ccgaaaagta
     5401 cctgccgggg gccgttcgcg ctccgggcat tgccgcccaa ccgcgcggcc gggcttacta
     5461 cgaagaacgg gtgcgctatt ttaccacctt gaacatgacg ccggaagagg tttttgaaac
     5521 cggacaacgc gaagtggccc gcatccgcgc cgaaatggag gccactctcc gcgagttgaa
     5581 gtatgccggt tctttcgccg atttcctgcg ttttctgcgc accgacccac agttttaccc
     5641 cgcatcgccg cgcgccctgc tcatggaagg ctcatattat gccaaaaaaa tagacggtaa
     5701 actgccacag tatttcggca agttgccccg caatccttac ggcgtttcac ctgtacccga
     5761 tattctggcg cctacttaca ccgcaggccg ctacagtccc ggcaacccca aagcacaacg
     5821 cgccgggttt tattggctca atacctataa cctgcccgcc cgaccgctgt atgcactgcc
     5881 cgcactcacc ctgcacgagg cggtgcccgg gcaccacctg caaatttcgc tggcgcagga
     5941 aatggaaaac gtacccgaat ttcggcgcaa tgcctatctc agcgctttcg gcgagggctg
     6001 ggcgctgtac ggcgagtggc tcggcaaaga aatgggcatt tacgaaacgc cttacgaaca
     6061 tttcgggcgg ctgacctacg aaatgtggcg tgcctgccgc ctcgtggttg atcccggcat
     6121 tcacgtcatg ggctgggacc gcgagaaggc catacagttt atgactgaaa ataccgcgct
     6181 ttccctccac gaatgcacca ccgaaataga ccgttacatc ggctggccgg ggcaggcggt
     6241 ttcgtacaaa ataggcgaac tgaaaatacg cgacttgcgc gccaaagcag aaaaagaact
     6301 cggcgacaag ttcgatatca gggcgttcca cgacctcgtc ctctccgaag gaaccgtacc
     6361 tttgtatgta cttgaaaacc ttgtagatga gtggattgcg caaataaaaa agaaataagc
     6421 gcgagcttcc cgacattttt ttcctccaaa gttgtcatcg cattgcaggg gttttatcat
     6481 ctttgcttta tcgcaaccgg cattttgttc aagccggtgc tgttccaata cgagcgactt
     6541 gccttatctc actgattatc aataatatca atttgcgaaa atcgcctaag gggcgaggta
     6601 tgcaacgaaa ggctgtcatt ctttcaaaaa ccaaataatt atgatgaaaa gagctttact
     6661 tttcgctttt tacagcttca tttcgctggc atgggcacag gcccaaacgt gtacgcccgc
     6721 tcagaatttt cccgacacgg ccatcgccat tccgccgtcc tggtcgccgc tccgtctcat
     6781 ggccggcatt caggataccg cctgcgtcgg tcagtacttc gagtttacgc tcagcatcaa
     6841 agcgccggca gctattccgg gtttgcccgg cgtcacggtc aattccattg ccataccggc
     6901 tgccaacggc gtagccaata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 cccccgcgat acctttgcct gcataaagat gttcggcatc cccaacaccc ccgccgatgt
     7021 ggggcaaaaa gacctcacgc tgtcgctgac gatcaatacg tcgtttggcc agttgagcaa
     7081 cattccttac ccgaatgcac agctcgatcc cggtggccac tactacctgc acgtcaagcc
     7141 gacggggtcg tccaactgct tcgtctcttc cgaccgcgag gccgatctgc cggttacggg
     7201 gttgtacagc cggcccaatc cgacttcggg ttatacccaa attcaggcca atgtcttgca
     7261 ggccgggcag tacgaatttc gggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nngtcattga tttcgacggc agccatttac cggcaggcat gtacctgtac
     7381 tcgctcaaca acggcagccg cgccgtgacg gagaagctga tcatcagccg gaggtagtga
     7441 ttgcgcgttg caaaagcgct ttgacgatca ttacagtcca aaattcacaa gcctcagacc
     7501 gctcattggg tggtctgagg cttgtacatt ttacagcgca tagacactat tctcaacgcc
     7561 ggctttctac tttccagccg ctttttttca attcctccac aaaaccggcg caactcggat
     7621 tgagcgcaat gttgccgggc gcgattttgg gaaggcggtt cgctttgaac ggcggcagct
     7681 ctgcgctcca gtcgcgttct gtgtac
//
LOCUS       DAORLF010000219         7660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5177_length_7660_cov_2.230243, whole genome shotgun sequence.
ACCESSION   DAORLF010000219 DAORLF010000000
VERSION     DAORLF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5177_length_7660_cov_2.230243"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1847)
                     /locus_tag="PK198_16240"
     CDS             complement(48..1847)
                     /locus_tag="PK198_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF40343.1"
                     /translation="MRTILKLSGLSLSLVLITACTTQKSRNDMTFLEKFHTNTNARYN
                     GYFNAKELMKVSTAALEQQHKDNYNEVLPMYKYLEADNPQAVAPDLDIAMKKVSIVVN
                     LYRRSKWTDDCYLLVGQAQYLKQDYESAESTFRFLTGEFDPNAPVAKKARTSSRSSSS
                     KESAAPKLTSKQKQKLAKQKAKERKKYNREIQLSRKKAAAAKAKGKTPPPPPTKPGQQ
                     DPKEAEKKAAAEAAQKAKEAAHKAKPDKGGHRSAFQAAELWLAKTLIERDNHEAAVRI
                     LNRLLENPKTYDDVRKEAVAVEAYAFASQKQYDRALPLLEQTIENTADKKQRTRYTYI
                     AGQLHQRAGRYSDAVAAYEQVLKQSADYEMSFNAGLNIALSSAAASGDPGAAVASLEK
                     MLKDVKNEEFKDQIYFALAQIALKAGNKEEGIKNLELALQNNKNNGAQKAEAYFTLAG
                     LYFEDESFVKAKKYYDSTLMVMPVADVRRLDAERMAENLADVAKYLEVINAQDSLLAL
                     AALSPKERADMAAQIKAERDEKRRAAAQSAAADANNAGKKFDKNAPFDAGLKGPGATV
                     TTASTFFAYNDREVRQGQRDFQRQWGNRLRCAR"
     gene            2018..>2450
                     /locus_tag="PK198_16245"
     CDS             2018..>2450
                     /locus_tag="PK198_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40344.1"
                     /translation="MASIKEKSFEWLGKLRNTYRLVVMNDETFQEVGSYKLSLLNVYI
                     FLSTVGVLLALAVGALIFFTPLKRYVPGYGSTSNDREMIRLNKQVEEMEKELSAFRTY
                     TDNVRRMLSGDVQTEKDVPKAKPSNAADSLAANPPEIPVVDE"
     assembly_gap    2451..2468
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2469..2916
                     /locus_tag="PK198_16250"
     CDS             <2469..2916
                     /locus_tag="PK198_16250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF40345.1"
                     /translation="ALNELGRAAQEPRTSNFSPRDVPLEQMFFSPPIKGKISAGFDAD
                     KKHYGTDVVAPQNTAIKSAMDGYVFFSDWTLETGNTIGIQHANNVITFYKHNSALLKK
                     NGAFVKAGEAIAIIGNTGTKTSGTHLHFELWHKGRPVNAVDYVKLE"
     gene            complement(2918..3508)
                     /locus_tag="PK198_16255"
     CDS             complement(2918..3508)
                     /locus_tag="PK198_16255"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRF40346.1"
                     /translation="MKMLHILCLGLVLAACGREAEPLETVSYTDEYGYTEKYSVKRFE
                     RSKEGLYQKSDAQGRLVEEANYLNDTLNGLRVLYYENGDTMSVEHYANGMFEGAFRSY
                     HENGRLELEGRYENNAMTGIWKRYYPDGQLMEEVTFKDNSENGPFTEYHPNGQLKAVG
                     EYLDGDNEHGELKIYDENGTLLRTMMCNYGRCKTVQ"
     gene            complement(3720..4877)
                     /locus_tag="PK198_16260"
     CDS             complement(3720..4877)
                     /locus_tag="PK198_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF40347.1"
                     /translation="MAKILIVDDEAAIRRTLREILQFEKYDVDEAADGLEGLVKVKQK
                     SYDVILLDIKMPKMDGMEALERIQELSPDTPVIMISGHGTIETAVEAVKKGAFDFLSK
                     PPDLNRLLITVRNALDKSSLIGETKTLKRAVSKSKVQEIIGQSPAIQHIKNTIERVAP
                     TDARVLVTGPNGSGKELIARWLHELSARSAQPIVEVNCAAIPSELIESELFGHEKGSF
                     TSAVKQRIGKFEQAHGGTLFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDISVDVR
                     IVAATNKDLRREIEKGNFREDLYHRLAVIIIESPALNDRREDIPLLADHFLRIVCNEY
                     GTALKRISPEAIRALQNFNWTGNIRELRNVVERLIILGGNPISEEDVNMYV"
     gene            complement(4895..>6587)
                     /locus_tag="PK198_16265"
     CDS             complement(4895..>6587)
                     /locus_tag="PK198_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF40348.1"
                     /translation="DLLKGKVTQVTKDGRFNAVINGAADWVYEEEFTLSQSYQWSPDG
                     KRIAFLRFDESAVKEFTMTNYLKELYPQYETFKYPKVGETNSTVTVFVHDVKSRKNRL
                     VQTGAEADSYIPRIKWTADPAQLCVYHLNRHQNKLRLLLADARTGKTSLLMEETNQYY
                     IEEGVLDDLYFFKDNKRFLWSSEKSGWRHVYLHDMSGKEIMPVTQGEWEVSQVYGVDE
                     ARQLVYLQAARRSAAERQVLSVSLEGKREQKLADQGGWNSAQFSSTFDYYVVTHSSIN
                     TPPSFAVFDSEGRLVRPIEDNAGLRKKQQEHEVNEVEFFNFTTGEGVELGGWMIRPRK
                     METGRRYPVLMYVYGGPGSQQANNMWKGANYWWFQMLAQQGIIVACVDNRGTGGRGEQ
                     FKKMTYLQLGKYETIDQIAAAKHLGSLPYVDAGRIGIFGWSYGGYMSSLCILKGADVF
                     KAAIAVAPVTNWKWYDTVYTERYMRTDKENSSGYEDNSPVNFADRLKGKYLLIHGMAD
                     DNVHFQHTAEMANALINANKQFDTYFYPNRNHGIYGGNTRIHLYSKMTEFLKENL"
     assembly_gap    6588..6597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6598..7075)
                     /locus_tag="PK198_16270"
     CDS             complement(<6598..7075)
                     /locus_tag="PK198_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRF40349.1"
                     /translation="MKRIIFSVWMLIFIAATAAAQAPITLEQIWQGGTLNARSLPGFN
                     FLKDGRHYTRLEKGKINQYDIRTGEVVATILDVSALSASAGYTGTIDSYTFSEDEQKI
                     LITTETEYIYRHSTRSKVSVYDRATKQLTPVFSQKKVMYPEFNPQGTAGAFVTANNL"
     assembly_gap    7405..7451
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7452..>7660
                     /locus_tag="PK198_16275"
     CDS             <7452..>7660
                     /locus_tag="PK198_16275"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40350.1"
                     /translation="MTLIDQIREMISEGETEKSLDELYKYVKENNAEIIDSLVMLRSR
                     LKNIEDGIIRGTMDDDDANLERAKV"
BASE COUNT         1613 a         1996 c         1973 g         2003 t
ORIGIN      
        1 cggtttgccc accaacgccg ttttagaaag tgtgctgaac atgtctatca ccgcgcacat
       61 cttaggcggt tgccccattg gcgctgaaaa tcgcgctggc cttgccgaac ttcccggtcg
      121 ttgtaggcaa aaaacgtact ggcggtagtt accgttgctc cggggccttt caaacccgca
      181 tcgaagggtg catttttatc aaacttcttg ccggcattat tggcatcggc ggcggcggac
      241 tgagctgctg cgcgtcgttt ttcatcgcgt tctgccttga tttgggcagc catatcggcc
      301 cgctctttag ggctgagcgc cgccagtgca aggaggctgt cttgtgcgtt gatgacttcg
      361 aggtacttgg ctacatcggc cagattttcg gccatgcgtt ctgcgtccaa gcggcgtacg
      421 tccgcaacag gcatcaccat cagggtactg tcgtagtatt ttttagcttt cacaaatgat
      481 tcgtcctcaa aatacagccc ggccagcgtg aaataggctt ctgctttttg ggcgccgttg
      541 ttcttgttgt tctgaagcgc cagttctaag tttttgatgc cttcttcttt gttgccggcc
      601 ttgagtgcta tttgagccaa cgcaaagtaa atctggtctt taaactcctc atttttaaca
      661 tccttgagca ttttttcgag gcttgccacc gccgcgcccg gatcgcccga agcggccgca
      721 ctgctcaacg ctatgttcaa gcctgcattg aacgacattt cataatcggc actttgcttg
      781 agcacctgct cgtaggcagc tacggcatcc gagtagcggc cggcccgctg gtgcaactgg
      841 ccggcgatgt aggtatagcg agtgcgttgc tttttgtcgg cagtattttc aatggtttgt
      901 tccagtaagg gcaatgcgcg atcatactgt ttttgggagg caaacgcata agcctctacg
      961 gctacggcct ctttacggac atcatcataa gtttttgggt tttccaacag cctgttcagt
     1021 atgcgaacag ccgcctcgtg gttgtctctt tcaatgagcg ttttggctaa ccagagctcg
     1081 gcggcctgaa atgccgagcg atgcccgcct ttgtctggct tggccttgtg ggcagcttct
     1141 ttggcttttt gagcagcttc ggcagccgcc tttttttcag cttctttggg gtcttgctgg
     1201 ccgggtttgg taggtggcgg cggcggggtt ttgcctttcg ctttggcagc agcggctttt
     1261 ttgcggctga gctgtatctc gcgattgtac tttttgcgct ccttggcttt ttgtttggcc
     1321 aacttttgct tttgcttgga cgtcagcttc ggtgcggcag attctttgga tgagctgctg
     1381 cgcgaactgg tgcgggcctt tttagcaacg ggtgcgttgg ggtcaaattc gcctgtaagg
     1441 aaacggaagg tggactcagc cgactcatag tcctgtttca gatattgagc ctgccccacc
     1501 agaaggtagc aatcgtcggt ccatttgctg cggcggtaca ggttgactac aatggacacc
     1561 tttttcatgg cgatatccag atcgggtgca acggcctgcg ggttgtcggc ttccaaatat
     1621 ttgtacatcg gcaacacctc gttgtagttg tctttgtgtt gctgttccag cgccgccgtg
     1681 ctgactttca tcagttcttt ggcattaaaa tagccgttgt agcgggcatt ggtgttggta
     1741 tgaaatttct ccaaaaaagt catgtcatta cgacttttct gagtagtaca ggcagtaatc
     1801 aataccagcg aaagggaaag tccggagagt ttaagaatcg ttctcaacac ggtgaaattt
     1861 aaagtgacgc taagccctat tgttgtacct ttgcacaaga cctgacgtgg tctgaaggcg
     1921 ggaccgcaca attagcgggc aaaattatcc gttttttgtc aactcatcaa accacaaaac
     1981 gcaaatctta gtatttgttg aattacaatt aacaggcatg gcctctatta aagaaaaaag
     2041 cttcgagtgg ctggggaaac tgcgcaacac ctaccgattg gtagtgatga acgacgagac
     2101 ttttcaggaa gttggctcct acaaactcag cctgctcaat gtctatattt tcctcagcac
     2161 ggtgggagtg ctgctggccc tggccgtagg ggcgctcatt ttctttacgc cgctcaaacg
     2221 ctatgtgccg ggttacggca gcacctccaa cgaccgcgag atgattcgcc tcaacaagca
     2281 ggtggaggaa atggaaaaag aactgtctgc attcagaacc tacaccgaca atgtgcggcg
     2341 catgctttcc ggcgatgtcc aaacagaaaa ggatgtgccc aaggcgaaac catccaacgc
     2401 agccgacagc ctcgccgcca acccgcccga aatccctgtt gtggacgaag nnnnnnnnnn
     2461 nnnnnnnngg ccctcaacga gctgggtcgc gccgctcagg aaccccgcac ctccaatttc
     2521 tcgccccgcg atgtaccctt agagcagatg ttcttctcgc cgcctatcaa aggaaaaatc
     2581 agcgccggct tcgatgccga taaaaaacac tacggcaccg acgtggtggc gccccaaaat
     2641 acagccataa aatcggctat ggacgggtat gtgttttttt ccgactggac gctggaaacc
     2701 ggcaatacca tcggcataca acacgccaac aacgtcatca ctttttacaa acacaactcg
     2761 gccctgctca agaaaaacgg ggccttcgtc aaagccggcg aagccatcgc catcatcggc
     2821 aatacaggca ctaagacctc cggcacgcac ctgcacttcg aactctggca caaaggccgc
     2881 cctgtaaatg cagtggacta tgtaaaattg gagtgagtta ttgcaccgtt ttgcagcgcc
     2941 cgtagttgca catcatggtg cgaagcagcg tgccgttttc gtcgtaaatt ttcagttctc
     3001 cgtgttcatt gtcgccgtct aagtactcgc ccacagcctt gagttggccg ttgggatggt
     3061 attccgtgaa ggggccgttt tcgctgttgt ctttaaaggt cacttcctcc atgagttggc
     3121 cgtcggggta gtatcgcttc cagatgccgg tcatggcatt gttttcatag cgcccctcca
     3181 actccaaacg cccgttttcg tggtaagacc ggaaggcccc ttcaaacatg ccgttggcgt
     3241 agtgctccac gctcatggtg tctccatttt cataatataa gacccgcagc ccgttcaggg
     3301 tgtcgttgag gtagttggcc tcctccacca accggccctg cgcgtcgctt ttttggtaca
     3361 aaccttcttt gctgcgctca aagcgcttga ccgaatattt ctccgtgtag ccatattcgt
     3421 ctgtgtaaga cacggtttcc agaggttcgg cttcccggcc gcaagctgca aggactaagc
     3481 cgagacataa gatgtggagc attttcattg gcgtgtgttt ttatggttca aggttccacg
     3541 ttccattcgt agataaactg cgcagcagat tacccctccc tctccgttgg cgataaactg
     3601 ccgaaggcag attcccctct ccgttggcaa tgaactgcgc agcagattac ttcaaagaag
     3661 ataaactgcg cagcagattt cccctccctc tccgttggcg atgaactgcc gaaggcagat
     3721 tacacataca tgttgacatc ctcctccgaa atgggattgc cgcccagaat aatcaggcgc
     3781 tccaccacat tgcgaagctc gcggatattg cccgtccaat tgaaattttg caatgcgcga
     3841 atggcctccg ggctgatgcg tttgagggct gtgccgtact cgttgcacac gatgcgcagg
     3901 aagtgatcgg ccagcagcgg aatgtcttcg cggcggtcgt tgagggccgg cgactctatg
     3961 atgataacag ccagccggtg atagaggtct tcgcgaaaat tgcctttttc gatttcccgg
     4021 cgcaggtctt tgttggtagc cgccacgatg cgcacgtcca ccgagatgtc tttttcgcca
     4081 cctacccgcg tgatgcggtt ttcttgtaga gcacgcaaca ctttggcctg tgccgagagg
     4141 ctcatgtcgc cgatttcatc gagaaacagg gtgccgccgt gcgcctgctc aaacttgccg
     4201 atgcgctgtt tgaccgccga ggtaaaggag cctttttcgt ggccaaacaa ttcgctttca
     4261 atgagttcgg agggaatggc tgcgcaattg acctccacga tgggctgtgc cgagcgggcg
     4321 ctgagttcgt gcagccaacg cgcgatcagt tccttgcccg aaccgttagg ccctgtgacc
     4381 aacacgcggg catcggtggg tgccacgcgt tcgatggtgt ttttgatgtg ctgtatggcc
     4441 ggcgactgcc cgatgatttc ctgcactttg gatttggaga cggcccgttt cagcgttttg
     4501 gtttcgccga tgaggctcga tttatctaag gcattgcgca ccgtgatgag cagacggttg
     4561 aggtcgggcg gtttggagag gaagtcgaaa gcgccttttt ttacagcttc caccgctgtt
     4621 tcaatggtgc cgtgcccgga aatcataatg accggtgtat cgggggacaa ctcctgaatg
     4681 cgctccaggg cctccatgcc gtccattttg ggcattttga tgtccaacaa aatgacgtcg
     4741 tagctttttt gtttcacttt caccaagcct tcgagaccgt cggcagcttc gtccacgtcg
     4801 tatttttcaa actgtaggat ttcgcggagg gtgcgccgga tagcggcttc gtcgtccacg
     4861 atcaatattt tggccatcgt gtgaattttt tgttttacaa attctccttc aaaaactccg
     4921 tcattttgga atacaggtga atgcgcgtat tgccgccata gatgccgtga ttgcggttgg
     4981 gatagaaata ggtgtcgaac tgtttattgg cgtttatgag ggcgttcgcc atttcggcgg
     5041 tgtgctgaaa gtgtacgttg tcgtcggcca tgccgtgtat aagcaggtat ttgcccttga
     5101 ggcggtcggc gaaattgacg ggcgaattgt cttcatagcc ggaagaattt tccttatcgg
     5161 tgcgcatgta gcgttctgtg taaacggtgt cgtaccattt ccagttggtc acgggagcca
     5221 cggcaatggc agccttgaac acatccgcgc ctttgagaat acacagcgaa gacatgtacc
     5281 cgccatagct ccagccgaaa ataccgatgc gcccggcgtc cacatacggc aatgagccaa
     5341 ggtgtttggc agcggcaatt tggtctatgg tttcatattt tccgagttgt aggtaggtca
     5401 tttttttaaa ctgttcgccc ctgccgccgg tgccccggtt gtccacacaa gccacaataa
     5461 tgccttgctg ggccagcatc tggaaccacc agtaattggc gcctttccac atattgttgg
     5521 cctgctgact gcccggacct ccatacacat acataagaac ggggtagcgc ctgccggttt
     5581 ccattttgcg cgggcgtatc atccagccgc ctaattctac cccctcgccg gtcgtaaaat
     5641 tgaaaaattc gacctcattc acctcatgct cttgctgctt tttgcgcaat cctgcattgt
     5701 cttcgatggg ccgcaccaag cgcccctcgc tgtcgaacac cgcaaaagac ggcggcgtgt
     5761 tgatggagga gtgcgtgacc acatagtaat cgaaagtgct gctgaattgc gcgctgttcc
     5821 aaccgccctg atcggccaat ttttgctcgc gtttgccctc caagctcacc gaaagcacct
     5881 ggcgctcggc cgccgaacga cgcgctgcct gcaaataaac gagctgccgc gcttcgtcca
     5941 cgccatacac ctgcgacact tcccactcgc cctgcgtcac cggcatgatc tccttgccgc
     6001 tcatgtcgtg caaatagacg tgccgccagc cgcttttttc gctgctccac aggaagcgtt
     6061 tgttgtcttt aaaaaaatac aaatcatcca gcaccccttc ttcgatgtag tactggttgg
     6121 tttcttccat cagcagggag gttttgcctg tgcgcgcatc ggccagcagg aggcgcagct
     6181 tgttttggtg gcgattgagg tgataaacgc agagttgcgc cgggtcggcg gtccatttga
     6241 tgcggggaat gtacgagtct gcttcggcgc cggtttgtac caacctgttt tttcgacttt
     6301 ttacatcgtg tacaaaaacc gtcactgtgg agttggtttc gcctactttg ggatacttga
     6361 aggtttcata ctgcgggtat aattctttga gataattggt catggtaaac tccttcaccg
     6421 cgctctcatc gaaacgcaaa aaagcgatgc gcttgccgtc gggcgaccac tgatagctct
     6481 gcgaaagcgt aaactcctct tcatacaccc agtcggcggc gccgttgatg acggcattaa
     6541 agcgcccgtc tttggttacc tgtgtgactt tgcctttcag caaatccnnn nnnnnnnaaa
     6601 ggttgttggc cgttacaaaa gcaccggcgg taccctgcgg attgaattcg gggtacatca
     6661 cttttttctg actaaaaaca ggcgtcagtt gcttcgtggc gcggtcataa acagatactt
     6721 tggagcgcgt ggaatggcgg tagatgtact ctgtttcggt ggtgatgagt attttttgct
     6781 cgtcttcgct gaaggtatag ctgtctatcg taccagtgta gcctgccgat gcgctgagcg
     6841 ccgacacatc aaggatggtg gccacgacct ctccggttcg aatatcatac tgattgatct
     6901 tgcccttttc caagcgggta taatgtcgcc cgtctttgag gaagttgaag cctggcagcg
     6961 aacgggcatt gagcgtaccg ccctgccata tttgttccaa cgtgatgggc gcttgagccg
     7021 cggcagttgc tgcgataaaa atgagcatcc agacagaaaa aatgatacgt ttcatatcgg
     7081 gtattttttt gaaggcgcta aaataggcat ttttccggca gtgccgacga tctttaaacc
     7141 gtgaacaaaa actacgcctt gccatgccgg ctaccgtgtg tatagctgaa gctgcacctc
     7201 atttattcat tataaaaatg tgtgctttat ttcgacaccg atcccaggaa aggactccct
     7261 ggatggcgag gcgaaaattt gacaaatctt cgagattttg tcaaattgtg tgctgcctga
     7321 aaactgtgcc ttattttacg caatctgttc tgcaaaccaa ccgcggcttt ctcatattgc
     7381 gacaaaaaac actacctttg agccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nccatgactc ttattgacca gattcgtgag atgatctccg agggcgaaac
     7501 agaaaaatct ttggacgaac tgtacaagta cgtcaaagaa aacaacgccg aaattattga
     7561 ctcgctcgtg atgctccgca gccgccttaa aaacatagaa gacggcatca ttcgcggcac
     7621 gatggatgac gacgacgcca acttggagcg cgccaaagtc
//
LOCUS       DAORLF010000220         7654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5182_length_7654_cov_2.115278, whole genome shotgun sequence.
ACCESSION   DAORLF010000220 DAORLF010000000
VERSION     DAORLF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5182_length_7654_cov_2.115278"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..666)
                     /locus_tag="PK198_16280"
     CDS             complement(127..666)
                     /locus_tag="PK198_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40351.1"
                     /translation="MAIRPDMNDSYIQIGHTGKPHGIAGELKIVVKERYERDALQANV
                     LFLDLKGSPVPFFVEDLSIGNAWIVKLEDVDSREHALSISGKGIFLRQSDLLPPEAAD
                     PDAEPDYELFVGYHLIDNEAGPIGPIEAVYDLGTQSLAGVRYQDREVLVPLHHHLIVD
                     IREDVGELVMDLPLGLLEL"
     gene            complement(657..1418)
                     /locus_tag="PK198_16285"
     CDS             complement(657..1418)
                     /locus_tag="PK198_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40352.1"
                     /translation="MTRFFFLLILLSAILACTRETETIETDLGREYFPLQVGRVWEYQ
                     VDSIIFDPVGNSVAVDSTRSWLREEIIALYVDAAGDTVFHTERYVRSDTTHNWQISKV
                     LALSQNTRQATRTEDNLRFVKMVFPLKTGARWNGNQYIDDALRITVAGEVIHIFKDWQ
                     SEVLDQSGAWSNGVLSFDDVAMIVHADSENLIEYRFVQEAYARNIGLIYNEMRILDTQ
                     CQACCNADFARCSSLPWTQKAEKGFIIKQRLIHWQ"
     gene            complement(1428..2132)
                     /locus_tag="PK198_16290"
     CDS             complement(1428..2132)
                     /locus_tag="PK198_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40353.1"
                     /translation="MNIFIRCFFCIAFGLLTTAAQAQAKYRLADYMPNHAGDSLLFQN
                     LNPAVKEPILIAWPDTATFKGQTVLERTESTGGRRLELLDPAEGWKLFLVGFQKGMDM
                     IFEKPLLLLPAEVEHGAVYRSSSPFSVFSSGRKLGAGIQNYEIKVQGHDTSRTPLRNF
                     GECLVIITTSTRTDTDGVRRGYEIKEWYARDFGLVKMAGEAFTLDAKGARTRVVKAAG
                     MLEKAKVAGESYKWND"
     gene            complement(2137..2586)
                     /locus_tag="PK198_16295"
     CDS             complement(2137..2586)
                     /locus_tag="PK198_16295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF40354.1"
                     /translation="MKKLWTMAAMAVAVLLFSHCEKDQLKIDRELILENLAEKGITAE
                     EHESGIFYVITTEGTGGHPSLASTVTVKYRGTLLNGNVFDQTVDPNTATFPLQNLIEG
                     WQIAIPLLKKGGKGTFWIPSALGYGDAALPGIPKNSVLVFDIELVNF"
     gene            3115..3516
                     /locus_tag="PK198_16300"
     CDS             3115..3516
                     /locus_tag="PK198_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRF40355.1"
                     /translation="MSRTYNIGDKASFSRQFDAQDVETFARISADDNPIHTDEDFAAA
                     SIFGKRVVHGIFTVSMFSKIFGTIYPGNGGIYLSQNTRFLRPVFVGDSVTAEVELIDF
                     QEEKRIGTFSTRCINGEGKIVADGEAKIKMP"
     gene            3513..4379
                     /locus_tag="PK198_16305"
     CDS             3513..4379
                     /locus_tag="PK198_16305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF40356.1"
                     /translation="MMQGKVCVVTGATAGIGYETALALAGKGAQVVVVGRSAEKGAAT
                     VAQIQQATGNTSVHFVKADMASQASIREAAADILRRFPVVDVLVNNAGTWVSDFTTTS
                     DGVETVFAVNHLAYFLFTHLLYPALRQAPDARVVNVASDSHFQTDLHLDDLNLSKNYS
                     GLRSYAQSKLANVMFTYEFERRKSDAQVSIYAVQPGLVHTDIGLKHTTWLHALAWKVR
                     RTFWKSKTPAEGAETSVFLASSDEARGQSGLYWDLCRPKRSSDASYNEANAARLWDIS
                     LEWSGIGKYFGR"
     gene            4474..>4949
                     /locus_tag="PK198_16310"
     CDS             4474..>4949
                     /locus_tag="PK198_16310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRF40357.1"
                     /translation="MNTPTITDIIRRRRSVYPKMYADRPLSEDIIREVLENANWAPTH
                     RLTQPWRFRVFRGDALRRLGEFLSGQYRAGTSEAEFSDLKYKKMLDAPQRSGAVIAVC
                     MQRDPENRVREWEEIAAVACAVQNMWLTCTSYGIGSYWSTPGYILQADEWLGLQPG"
     assembly_gap    4950..4989
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5080..5451
                     /locus_tag="PK198_16315"
     CDS             5080..5451
                     /locus_tag="PK198_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40358.1"
                     /translation="MIPVDRQALYLAPKKPLTDWVNYVFPDEEKVSPEEPLANDWGTV
                     YLVPIFNSEEEYEAWMQENFQAFFETELYDWCEDETQWPSPLTMELFQEWFHVSFQSV
                     VLDTIDLPITKDDDEEDDWDF"
     gene            5472..5684
                     /locus_tag="PK198_16320"
     CDS             5472..5684
                     /locus_tag="PK198_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40359.1"
                     /translation="MIYITIAGSAQVLVLPKQQKTAIMYLVDWLQANLLMVFTTLVLI
                     GIPAIIGIVVFNNMSKKKGGDTDATE"
     gene            5925..6500
                     /locus_tag="PK198_16325"
     CDS             5925..6500
                     /locus_tag="PK198_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40360.1"
                     /translation="MKRLLLMIFLWLTTLAAAHAQCKVAVETIDEFDSTRLVVSQPVN
                     IGNMIPSNHRNADGMLMVEEAKVLFSFTENDTLGAFFLTIAAAEREYYTTEEGYNIYL
                     IMADGTLLEMMNYPDKGAMDDKTLMRIYTHTCVAPVQYFFIMADTYIDRIRILYKGHK
                     RTIKLTPEQQEEARKAIRCVGEKLYFYPIKP"
     gene            complement(6513..7616)
                     /locus_tag="PK198_16330"
     CDS             complement(6513..7616)
                     /locus_tag="PK198_16330"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRF40361.1"
                     /translation="MHILPFKALFPNMEYIPSPDYFFATVREEFPQYYDNGFFWSIEK
                     EAFFVYEIQTPRRNYLGLAACVDLRDYREGAIKKHEQTIAENEQKQMQLALKRKAGVK
                     PVLLTYPEVPDIQNWLRQHTLDREPALAVHFEEDAQHHRLWAVSEADDMESIRQLFLE
                     KVCCSYIADGHHRIATTALMHEKLETPEGQRHYGTLLSAFFSSEELEILDFNRVIEAF
                     EDISPTQFMARLSQICEIELMSAPEKPSQKHELTMYLRREWYRLRWRPEILTRQGADC
                     LDAALLNELVLKEIVGITDVRHDLRVEYVEGPKGLDGLRKKAMKNEQTVAFCLFPIQM
                     PDLFRLADAEQMLPPKSTWFEPRMKNGMIVQEF"
BASE COUNT         1834 a         1919 c         1975 g         1886 t
ORIGIN      
        1 gccgtaacgc ttgcacaaag cgcggaaagg cggatcgctg acgtcttcca taggcgccag
       61 cagcagcgga tattcgccga gttctacgtt tccaatttta accatgccgc aaaggtacgc
      121 tgaaagtcac aactccaaaa gccccagtgg caaatccatg accaactcgc cgacgtcttc
      181 ccggatgtcc acgatgaggt gatggtgtag ggggactagt acttcccggt cctgataacg
      241 cacgccggcc agcgattgag tgccgaggtc gtaaacagcc tcaatggggc cgatgggacc
      301 ggcttcgttg tctatgagat ggtatcctac aaagagttcg tagtcgggtt cggcgtcagg
      361 gtcggcggct tcgggaggca gcaggtcgga ctgtcgcagg aaaatgccct tgccggaaat
      421 gctgagggcg tgttcgcggg agtccacgtc ttccaatttg acgatccagg cattgccgat
      481 gctgaggtct tccacaaaaa aaggcaccgg actgcctttg aggtctaaaa aaaggacatt
      541 ggcctgcagt gcgtcgcgct cgtagcgttc tttgacgacg attttcaatt cgccggcaat
      601 gccgtgcggt ttgccggtat ggcctatttg gatgtagctg tcgttcatat cgggccttat
      661 tgccaatgaa tcaatcgttg tttgataatg aagccctttt cggctttttg agtccagggc
      721 aggctgctgc accgtgcaaa gtcggcattg caacaagcct ggcattgggt atcaaggatg
      781 cgcatttcgt tgtatatcaa tccgatattg cgggcgtaag cctcttgcac aaatcggtac
      841 tcgatgaggt tttcgctgtc ggcatggaca atcatcgcca catcgtcaaa agaaaggacg
      901 ccgttcgacc atgcgccgga ttgatctaac acttccgatt gccaatcctt aaaaatgtgt
      961 atcacctcgc cggctaccgt tatccgcagc gcgtcgtcaa tgtattgatt gccgttccag
     1021 cgggcgcctg ttttcagcgg gaagaccatt ttcacaaaac gcaggttgtc ctccgtccgg
     1081 gtggcctgcc gggtgttttg actcagggct aatactttgg aaatctgcca gttgtgtgtg
     1141 gtatcgctgc gcacatatcg ctcggtatga aaaacggtat ctccggcggc gtccacatac
     1201 agagcgatga tttcttcccg cagccaggaa cgcgtgctgt ccaccgctac gctgttgccc
     1261 accggatcga agatgataga gtccacctga tattcccaaa cccggcccac ctgcaaggga
     1321 aaatactccc gaccgaggtc ggtttcaatg gtttccgtct cgcgggtgca ggcgaggatc
     1381 gcggaaagca ggatcaggag gaaaaaaaat cgcgtcatgg catccgctta atcattccac
     1441 ttataactct cgccggccac tttggccttt tccagcatac cggctgcttt caccacgcgg
     1501 gtacgggcgc ctttggcatc aagagtgaag gcttcgccgg ccattttgac gaggccgaaa
     1561 tcgcgggcat accactcttt gatctcgtag cccctgcgca cgccgtcggt atcggttcgg
     1621 gtggaagtag tgatgatgac caagcactcg ccgaaattgc gcagcggcgt gcggctcgtg
     1681 tcgtggcctt gtactttaat ctcgtagttt tgaattcccg cgccgagttt gcgccccgaa
     1741 gaaaaaacgg aaaacggcga agacgagcga tatacagcgc cgtgctctac ctcggcgggt
     1801 aacaagagca gcggtttttc aaaaatcatg tccatgcctt tctgaaatcc gacgaggaac
     1861 aacttccaac cctccgcagg gtccagcagt tccaatcgcc ggccaccggt gctttcggtg
     1921 cgctccaata cggtttgtcc tttaaaagtg gccgtatcgg gccaggcaat gaggatcggc
     1981 tccttgacgg cgggattgag gttttggaac aacaggctgt caccggcgtg attgggcatg
     2041 tagtcggcga gacgatattt ggcttgcgcc tgcgcggctg ttgtcagtaa gccgaacgcg
     2101 atgcagaaaa agcaacggat aaaaatgttc atggaatcag aaattgacca actcgatatc
     2161 gaatacgagc acggagtttt tggggatgcc cggcaacgcc gcgtctccgt atcctaacgc
     2221 agaaggaatc cagaacgtgc ctttgccgcc ttttttaagc agcggaatgg caatttgcca
     2281 gccttcgatg aggttttgaa gcgggaaggt agctgtgttg ggatccaccg tttggtcaaa
     2341 aacgtttcca ttgagcaaag taccgcggta ttttaccgtc acagtagagg cgagactcgg
     2401 gtggccgcct gttccctctg tggtgatgac gtaaaaaatg ccggattcgt gttcctccgc
     2461 cgtgatgcct ttttcggcta agttttcgag gatcagttcg cgatcaatct tgagttgatc
     2521 tttttcgcaa tgggaaaaga gcaatacagc gacggccatt gcggccattg tccatagttt
     2581 tttcatgttt gagtattttt ttgaggggca aaggtaatgg ttttttattg ggcgtcgtcg
     2641 ggggtacaca aggatcgttt cgacagtacc ttgcatcgct ccctgttctg cccccaaaaa
     2701 taaaaagagg cagcactcca agagtactac ctcagcccta accaaataaa accaaattat
     2761 ttcaaaccct ttcgggtaat tgtattgaaa cggtcgttga cttgcttatt tcgatcctcg
     2821 tttggatgct ttagcaaaag cgacgctgca aagatacgca ctttgtgtac aacatacaat
     2881 tacaaaggcc aaaaaaaatt tcactttttt ttagaaacct gtcgaaatac ccccatgatt
     2941 gttaaaatgt atcaaattgg atgagccgca agtgccatga aatcgccgcc ggattctgct
     3001 gtgtgcgagc cgtatccata cgccgtcaga tgtttatgtc gcccaagccg cgcttcgcat
     3061 cctgataata tcgtatcttt gcatgctttt tttcacggca aacaaagttt cggcatgagc
     3121 agaacataca acatcggcga taaagcctct ttttcccggc agtttgacgc gcaggacgta
     3181 gaaaccttcg cccgcatcag cgccgatgac aatcccatcc acacagatga ggactttgcc
     3241 gccgcttcca ttttcggcaa gcgagtggtg catggcattt ttacggtgtc tatgttttcc
     3301 aaaatattcg gcaccattta tcccggcaac ggaggcatct atctgagcca aaacacccgg
     3361 tttctgcggc ccgtttttgt gggtgacagc gtgacggcag aagtggaatt aattgatttt
     3421 caagaagaaa agagaattgg cacgttcagt acccgctgca tcaacggaga aggcaagatc
     3481 gttgccgacg gtgaggccaa aataaaaatg ccatgatgca agggaaagta tgcgtcgtca
     3541 ccggggctac ggcgggcatc ggctatgaaa cggcgctggc cctggcaggc aaaggtgctc
     3601 aggtggtagt cgtgggacga agtgccgaaa aaggcgctgc gacagtggca caaatacagc
     3661 aagcgaccgg caataccagc gtacattttg tcaaagctga catggcctcg caagcctcca
     3721 tccgcgaagc tgccgccgac attttgcgcc gcttccctgt ggtggatgtg ctggtgaaca
     3781 acgccggcac ctgggtatct gatttcacta ctacatcgga cggcgtggaa acggtttttg
     3841 ccgtcaatca tctggcgtat tttttgttca cacacttgtt atatccggct ttgcgccaag
     3901 cccccgacgc ccgcgtggtg aatgtagctt ctgattctca ttttcaaacc gacctgcatt
     3961 tagacgactt gaacctgagc aaaaactact ccgggcttcg ctcgtatgcg caatccaagc
     4021 tggccaatgt gatgtttact tacgagtttg agcggcgcaa atccgatgca caggtttcga
     4081 tctatgccgt tcagcccggt ttggtacaca ccgacatcgg actgaaacac accacctggc
     4141 tgcatgcgct ggcttggaaa gtgcgccgta ctttctggaa aagcaaaacg ccggccgagg
     4201 gcgccgagac ttccgttttt ctggcttcct cggatgaagc gcgcggacag agcggccttt
     4261 actgggactt gtgccgcccg aaacggtctt cagacgcctc ttacaacgaa gccaatgccg
     4321 cccgtttgtg ggacatcagc ttagagtggt caggaatcgg gaagtatttt gggaggtagt
     4381 ttttgttttt ctattggtta gtgaaggggt gactacgggc ttatgatcgc ggcggagtca
     4441 ccccttcact aaccgccaaa tatttcaggt tttatgaaca caccaaccat cacagacatc
     4501 atccgccgtc gccgctcggt ttatccaaag atgtacgcag atcggcccct ttcggaagac
     4561 atcatccggg aggtattgga aaacgccaac tgggcgccta cgcaccgcct cacccaaccc
     4621 tggaggtttc gcgtgtttcg cggggatgcg ctccgtcgtc tcggcgagtt tttatcgggg
     4681 cagtaccggg ccggcacgtc cgaagccgag ttttctgacc tgaaatataa aaaaatgctg
     4741 gatgcgccac aacgttcggg cgccgtcatc gctgtttgta tgcagcgcga ccccgaaaac
     4801 agggtgcgtg agtgggaaga aatagccgcc gtagcctgtg ccgtgcaaaa tatgtggctc
     4861 acctgtacgt cttatggcat aggttcgtat tggagtacgc cgggatacat tctgcaagca
     4921 gacgaatggc tcggccttca accgggcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnna tatgggctgg tacgatgagg tggaactgcc ggccaagcgt ggcagtatag
     5041 aagataaaac attgtggatc aacgaataaa attaaaataa tgatacctgt agatcgtcag
     5101 gccctgtatt tggcgcccaa aaaaccactc acggattggg tcaactacgt ctttcccgat
     5161 gaagaaaaag tcagccccga agagccatta gccaatgact ggggcacggt gtatctcgtt
     5221 ccgatattca attccgaaga ggagtatgaa gcctggatgc aggaaaattt tcaggcgttt
     5281 tttgaaacgg agctatacga ctggtgcgaa gacgaaacgc agtggcccag cccgctgacg
     5341 atggagcttt ttcaggaatg gtttcacgtc tcgtttcagt cggtggtgtt ggataccatt
     5401 gatttgccca ttaccaaaga cgacgacgaa gaggacgact gggatttttg agataaaagt
     5461 caatccttac ggtgatttat atcaccatag cgggttctgc gcaagtatta gttttgccca
     5521 aacaacaaaa aacagcaatt atgtatctgg ttgactggct gcaagctaat ttattgatgg
     5581 tattcaccac gctggtattg attggcatac ccgccatcat cggtatcgtt gtattcaaca
     5641 acatgtctaa gaaaaaaggt ggagacaccg acgcgactga gtaattcctg caatggcatt
     5701 ctgctaagta gtggggcaaa ataaatattc attttcttta ggccaaagga ttctctaaga
     5761 cacttaaaat gaagcgttta cttagcgaat cctttggcga aaatgaagat ttattttttc
     5821 tcgcccctta tgctctttga ttccgcatgt ttcatcaggt tgcatcgtgg agggaagggt
     5881 gtttttgtgc aaagtttcct acctttgtct aaaattccca aaacatgaag cgtctgttgc
     5941 tgatgatctt tttgtggctg acgaccctcg ccgccgctca tgcccagtgc aaagttgccg
     6001 tagaaaccat tgatgaattt gacagcaccc gcttggtcgt ttcccaaccg gtgaatatcg
     6061 gcaatatgat tcccagcaac catcgcaatg ccgacgggat gttgatggtg gaggaagcta
     6121 aagtgctgtt tagctttact gaaaacgaca ccctcggcgc gtttttcctc accatagccg
     6181 ccgcagaacg cgaatattac accactgaag aaggctataa tatttatctc atcatggccg
     6241 acggcacctt gttggaaatg atgaactacc ccgacaaagg agccatggac gacaagacgc
     6301 tcatgcgcat ctatacgcat acctgcgtgg cgcctgtgca atactttttc atcatggccg
     6361 atacctatat agaccgcatc cgaatactct ataaagggca caaacgcacc atcaaactca
     6421 cgccggaaca gcaggaggaa gcgcgcaaag ccattcgatg cgtaggggaa aagctgtatt
     6481 tttatccaat aaagccctga tcataattat tcttaaaact cctgcacaat cattccattt
     6541 ttcatgcgag gttcaaacca ggtggacttg ggcggcagca tttgttccgc atctgccagg
     6601 cgaaacaggt cgggcatttg aatgggaaac agacaaaacg ctaccgtctg ctcgtttttc
     6661 atggcctttt tgcgcaagcc atccagtcct ttcggcccct ctacatactc tacccggagg
     6721 tcgtgccgta catctgtaat gccgactatc tctttcaaaa ccagttcgtt cagcaatgcg
     6781 gcatctaagc aatcggcgcc ctgccgggtt aaaatttcag gtctccaacg cagcctgtac
     6841 cactcgcggc gcaggtacat ggtcagttcg tgtttttgac tgggcttttc gggggcgctc
     6901 atcagttcta tttcgcatat ctgcgacaag cgggccatga actgcgtagg cgaaatgtct
     6961 tcaaatgcct ctatcacccg gttgaagtcc aatatttcca gctcttccga cgaaaaaaag
     7021 gccgacaaca aagtgccgta gtggcgttgg ccttcgggcg tttccaattt ttcgtgcatc
     7081 agagctgtgg tggcaatgcg gtggtgcccg tcggcaatgt aagagcagca cactttttcc
     7141 aaaaaaagtt gcctgatgga ctccatgtca tcggcttcgc tcacagccca caagcggtga
     7201 tgctgggcgt cttcttcaaa atgcactgcc agagccggtt cgcggtcgag ggtgtgttgc
     7261 cgaagccagt tctgaatatc gggcacctcc gggtaggtga gcaataccgg ttttacaccc
     7321 gctttgcgct tgagggccaa ctgcatctgc ttctgctcat tttcggcaat cgtttgctcg
     7381 tgctttttga tggcgccctc ccggtaatcg cgcaagtcca cacaagcggc caatccgagg
     7441 tagtttcgcc ggggtgtttg gatttcatac acaaaaaagg cctccttttc gatgctccaa
     7501 aaaaagccgt tgtcgtagta ttgaggaaat tcttcgcgca cggtggcaaa aaaataatcg
     7561 ggcgaaggaa tgtattccat gttgggaaac agcgctttaa agggaagtat gtgcatggcc
     7621 ggcattttta ttttaaaaaa gggaactcaa aaac
//
LOCUS       DAORLF010000221         7612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5223_length_7612_cov_1.820034, whole genome shotgun sequence.
ACCESSION   DAORLF010000221 DAORLF010000000
VERSION     DAORLF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_7612_cov_1.820034"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="PK198_16335"
     CDS             <1..1582
                     /locus_tag="PK198_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRF40362.1"
                     /translation="SAGQKPAIIAENFNIMTMAAATDTETLISRLEDILQFVESREAD
                     FADLIQQVQPHYRDSARNLVHYMALRSFDLRPIQEELSMMSISSLGHSEGYTLTNLKK
                     ILHLLRLVEGLRDYGVQEDFHSPLNYYRSKEQLQENTLQLFGAHPGHNSTRIMVTMPR
                     EAAYDYDLVRSLLKSGMNIARINTSHEEPAEWEAMIRHIRRAEADLGYNCLVYMDLSG
                     PKLRTEQLDPAHTISESRKGDYLLLRQGDRISVVRQLPERLPMSETAANTFLSISLPQ
                     VFEDVQPDQRIFFDDGKIRAKITEVHPDSFEVEITYAGINGAKLRADKGINLPDTHLR
                     LPSLTEEDHDHLAFMARQADIVGYSFVRTPEDVEQLQSELAKLGREDMGIILKIETKE
                     AFENLPKMLLTAMRSPKTGVMIARGDLAVEMGFERIAEVQEEILWICESAHIPGIWAT
                     QVLEKLASKGIATRAEITDAAMSARAECVMLNKGPYIVDAVATLDDIIRRMASHQFKR
                     MSNLRPLQVANQFINNRS"
     gene            complement(1590..2657)
                     /locus_tag="PK198_16340"
     CDS             complement(1590..2657)
                     /locus_tag="PK198_16340"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRF40363.1"
                     /translation="MHLPAISFITAFALTFFAIPPIIEIARTKHLFDIPGERSSHKVA
                     TPSLGGVAIFAGAVFSIVLWTPFKDFANLQYILGAFLILFLIGVKDDISPVSPGKKMI
                     AQLLAAAILIFRSDIRLEGMYGLLGFHDELGGLVFILLSFFTIIVIVNAFNLLDGIDG
                     LAGSVGAIIMVTFGVWFYLTGHVELSTVAFASAGAVAAFLRFNYSPARIFMGDTGSLF
                     IGLVCAIMSIRFINFNYALEDGHPYKFAGTPAVAIGIMMLPLFDTLRVFITRIFRGHS
                     PFHPDRRHIHHMLIDFGMTHLQATALLVFVQMLFTVLAFSFHYSLGLHRLLLLEIILA
                     IGMTYWLHLNIVKHRRFRLPE"
     gene            complement(2681..>2981)
                     /locus_tag="PK198_16345"
     CDS             complement(2681..>2981)
                     /locus_tag="PK198_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRF40364.1"
                     /translation="YDCQTFIGTCIDRCDYEVDEDVARHFDAEFAEWNAEKGKFQLDL
                     KAANKAQLLAQGVQNQNIEVSPYSTASCVADFFSHRAEKGITGRMLAVIAMKNVP"
     assembly_gap    2982..2991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2992..3446)
                     /locus_tag="PK198_16350"
     CDS             complement(<2992..3446)
                     /locus_tag="PK198_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRF40365.1"
                     /translation="MLDIAFKPSILLAHLPDVVAGQSTRHGGVSEGAYASLNLGISTA
                     DDPQAVAENRRRFFAALGFDAAQTASAHQVHGDAVLHVQAPGRYDGYDALVTTKKDLL
                     LNVTVADCTPILIYDPVNQAIAAVHAGWKGTVLQIVAKTLQKMETLFGTR"
     gene            complement(3578..>4054)
                     /locus_tag="PK198_16355"
     CDS             complement(3578..>4054)
                     /locus_tag="PK198_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRF40366.1"
                     /translation="KALQQLTSQVMDAFGMRYSASHTEFIRCHEDGKFYFLETSCRVG
                     GAHISEMVEYSSGINLWAEWAKVEYAQASGGSYTMPTAKNLYAGLVVSLSRYTHPDYS
                     SFQDAELAWRLKKDQHIGLIITSDRRERVLELLDNYTRRIFEEFHASLPPKDKALS"
     assembly_gap    4055..4177
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4178..4807)
                     /locus_tag="PK198_16360"
     CDS             complement(<4178..4807)
                     /locus_tag="PK198_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRF40367.1"
                     /translation="MSKPFTFLCVATFFKGADFIRTAKMLGANVLVITKKSLEHEAWP
                     RESIDEFFYLEHDNNSPENMDMLREGLAYVMRSRKIDRVVSLDDFDVEKAAFLREEFR
                     IPGMGQTTAKYFRDKLAMRMRAQEAGIPVPAFSAVFNDEEVNHYLGTVPGPWVIKPRS
                     EASAIGIKKVHHAHDAWQVLHEIGSERHLFLIEQFKPGDVYHVDALSSDG"
     gene            complement(4824..>5798)
                     /locus_tag="PK198_16365"
     CDS             complement(4824..>5798)
                     /locus_tag="PK198_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRF40368.1"
                     /translation="RIVADTQSRIFDLVNAPANKKLPGDMAAWAEESAQWYNYTVDIL
                     GKNQLQALGFHALLAVNQGSAHPPCLIVAQYCPEKPVAKIALVGKGVTFDTGGLSIKP
                     SANMYLMKSDMGGAAAVMGAVEAAARLKLPVQIIGVVPATENSVDAHSMRPSDVIGSY
                     SGKTIEVIDTDAEGRLVLADGLFYAVKHYKPDVLIDLATLTGATVRAMGYHAGGLLSN
                     DDALANELMQAGERCGERLWRFPLWDAYKDEIKSDVADVRNLGIRPVAGAISAAKFLE
                     VFIDNHPRWAHLDIAGVALQDSEFSSQKSATAYGVRLLLEYFSSFSPS"
     assembly_gap    5799..5878
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5879..6300)
                     /locus_tag="PK198_16370"
     CDS             complement(<5879..6300)
                     /locus_tag="PK198_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRF40369.1"
                     /translation="MEIAITHQPDHHEYVVVIPVTQHHNLSEVLDLCRRENVTGIPEP
                     GQFKADWNEVWPMYKGEQRLFLLGLGANPGFPEVLKAFRSFGHKFRQRLSSDLAVSFL
                     YANMPDKPENVVEAAVNGLALSAYRIGKFKTSNGEEHPL"
     gene            complement(6382..>7258)
                     /locus_tag="PK198_16375"
     CDS             complement(6382..>7258)
                     /locus_tag="PK198_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF40370.1"
                     /translation="ENEELDEFGKTPHNRKANPQAGRIAVQVPRWRSQALYYKPELLP
                     RIKVIDEAFEIPQLIKTRRIAALLPHDYYETDRRYPVLYLQDGQNLFDDYAPFGNWAV
                     DKKLAMMSEKGQGDIIVVSIDHAESERIAEFTPSMRTRLGSGDGKKYVRFLADTLKPF
                     VDKEFRTLPDREHTGIGGSSMGGLISIYAGLMYPEAYSRLMIFSPSLWVVPNIHFQAL
                     TFTEQFASRIYLYGGENEGANVVPNIKRFVAAMEKQGFSANVDFNLAIDPKGGHNEAR
                     WGEEFPKAVEWLFFQ"
     assembly_gap    7259..7278
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7279..7488)
                     /locus_tag="PK198_16380"
     CDS             complement(<7279..7488)
                     /locus_tag="PK198_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40371.1"
                     /translation="MIESTTQRQANLTIELSTSEDSELPVFLTGNFNNWLVPDKRFLM
                     RKLEHGKYEFSFPPDLHLPDLVEYKF"
BASE COUNT         1958 a         1896 c         1769 g         1756 t
ORIGIN      
        1 gtctgccggg caaaaacccg ccatcattgc agaaaatttt aacatcatga caatggctgc
       61 tgctaccgat actgaaacgc tcatttcccg gctcgaagac attctgcaat ttgtagaatc
      121 ccgggaagct gattttgctg atctgataca acaagttcag ccccattacc gcgacagcgc
      181 ccgcaactta gtgcattaca tggccctacg ttcgttcgac ctgcgcccca ttcaggaaga
      241 actttccatg atgagcatat cctcgctggg gcactccgag ggttacacgc ttaccaattt
      301 gaaaaaaatt ctccacctgc tgcggcttgt ggaagggctg agggattacg gtgtgcagga
      361 agacttccac tcgccgctga attactaccg aagcaaggag caattgcagg aaaataccct
      421 ccaattgttt ggcgcacatc cagggcacaa cagcacccgc atcatggtga ccatgcccag
      481 agaagctgcc tacgactacg atctggttcg atctctgctc aaaagcggca tgaacatagc
      541 ccgcatcaat accagccacg aagagccggc cgagtgggag gccatgatcc ggcacatccg
      601 cagagctgag gcagatttag gatacaattg tttggtttat atggatttat ccggccctaa
      661 actacgaaca gaacaactcg accccgcaca taccatcagt gaatcccgca aaggagacta
      721 ccttttgttg cgtcagggcg accgaatctc ggtggtcagg caactgccgg agcgcctgcc
      781 gatgagtgaa acagccgcca atacctttct cagcatctcc ctgcctcagg tttttgaaga
      841 tgtacaaccg gatcaacgca ttttttttga cgatggaaaa atcagggcca aaatcacaga
      901 agtgcatcct gattcttttg aagttgaaat cacctatgcg ggcataaacg gcgccaaact
      961 ccgcgccgac aaaggcatca acctgcccga tacgcacctt cgcctgccct cgctcacaga
     1021 ggaggaccac gatcatttgg cgttcatggc gaggcaagct gatattgtgg gatattcttt
     1081 tgtcagaact cccgaagatg tggagcagtt gcagtcggaa ctcgccaaat tgggaagaga
     1141 ggacatgggc attatactca aaatcgagac caaagaagcc tttgaaaacc tgcccaaaat
     1201 gttgctcacg gccatgcgca gccccaaaac cggcgtgatg atagcgcgcg gcgatctggc
     1261 cgtggaaatg gggtttgaac gcatcgccga agtacaggaa gaaattctgt ggatttgcga
     1321 atcggctcat attccgggga tatgggctac gcaggttttg gaaaaattag catccaaagg
     1381 cattgctacc cgcgctgaaa tcaccgatgc ggccatgtcg gcacgggcag aatgcgtgat
     1441 gctgaacaaa ggtccgtaca tcgtggatgc agtagccacg cttgacgaca tcattcgccg
     1501 tatggccagt caccagttca aacgcatgag caatctgcgc ccgctacagg tggccaatca
     1561 attcatcaat aatagaagct gaggggcgct tattcaggca atcggaaacg cctgtgtttt
     1621 acaatattca gatgcaacca ataggtcatt ccgatggcca aaatgatttc cagcaacaat
     1681 aaacggtgca agcccagtga ataatggaag gagaatgcca gcaccgtaaa cagcatctgt
     1741 acgaacacca acaaagccgt agcctgtaga tgcgtcatcc caaaatcaat gagcatgtgg
     1801 tgtatgtgcc ggcgatcggg gtgaaacggc gaatgccccc gaaatatcct cgtgataaaa
     1861 actctgagtg tatcaaatag aggcagcatc ataatgccta tggcaacagc gggcgttccg
     1921 gcaaatttgt aaggatgccc atcctctaaa gcgtaattga aattgataaa ccgaatgctc
     1981 atgatagcgc ataccaagcc aataaacaga gagcctgtat cgcccatgaa aatacgcgcc
     2041 gggctgtaat tgaatctcaa aaatgccgct acggctcccg cagatgcaaa ggctaccgta
     2101 ctcaactcca catgccccgt gagataaaac cagacgccaa aagtgaccat aatgatggcg
     2161 cccacactcc cggccagccc atctattcca tccaataaat tgaaagcatt gacgatgacg
     2221 atgatggtaa aaaaagataa taaaatgaat acaagtcctc ccaactcgtc gtgaaaccct
     2281 aataagccat acatgccttc caagcgaata tcagagcgaa atatcaggat ggctgccgcc
     2341 aataactggg cgatcatttt tttgccgggc gacaccggcg aaatgtcatc cttgacgccg
     2401 atgagaaaaa ggatcagaaa agcgccgagg atatactgta aattggcaaa gtctttaaaa
     2461 ggcgtccaga gtacgataga gaaaacagcg ccggcaaaaa tggccactcc tcccaacgat
     2521 ggcgtagcaa ctttatgaga actgcgctcg ccgggtatat caaacaggtg cttcgtcctg
     2581 gctatttcaa taataggagg aatggcaaaa aaggtgagcg caaaggctgt tataaaactg
     2641 atggccggca aatgcatttc tcatgggagg attgtggggg ctaaggtacg tttttcatgg
     2701 ctattactgc caacattctt cccgttattc ctttttcggc ccggtgcgaa aaaaaatctg
     2761 ccacacaaga tgctgtggaa taaggcgaca cttcgatatt ttgattttga acgccttgag
     2821 cgagcaattg tgctttgttg gcggctttta aatccaactg aaatttccct ttctctgcgt
     2881 tccattcagc aaactcagca tcgaaatgcc gggccacatc ttcgtccacc tcataatcgc
     2941 aacgatcaat gcaggttccg ataaaggttt ggcaatcata tnnnnnnnnn ncgtgtgccg
     3001 aatagggtct ccattttctg aagagttttg gctactatct gcaaaaccgt gcctttccac
     3061 cccgcatgaa ccgccgcaat agcctgattg acaggatcgt aaataaggat gggcgtgcaa
     3121 tcggcaaccg ttacattgag gagaaggtct tttttagttg taaccaaagc gtcgtagcca
     3181 tcgtaacgtc ccggcgcctg aacgtgcaaa acagcatcgc catgcacctg atgagctgag
     3241 gctgtttgcg ctgcgtcgaa gccaagtgct gcaaaaaaac ggcggcgatt ttctgccacg
     3301 gcctgcggat catcggcggt gctgatgccc agattgagcg acgcatatgc gccttcgctc
     3361 actccgccgt gccgggtact ttggcccgcc acaacatcag ggagatgggc caacaagatg
     3421 gaaggcttga aagcaatgtc taacatgaga aattggtttt caaccggggg gatgcaaatc
     3481 actgctcttt ggtcatttca gacggttgaa taaacaagtg tttgaaaaat gcgtcgtggc
     3541 tttgcgtgac gggcttttcc atgtagtctg attattatca ggacaaagct ttgtccttag
     3601 gtggcagact cgcatgaaac tcctcaaaaa tgcgccgggt gtaattgtcc aacaattcca
     3661 atacccgctc tctgcgatcc gaggtgatga tcaagccaat gtgttgatcc tttttcaggc
     3721 gccatgccaa ttcagcgtcc tgaaatgaac tgtaatcagg atgggtatat cgagagagag
     3781 agaccaccaa tccggcataa aggttcttgg cagtcggcat ggtatagctg ccgccggagg
     3841 cttgtgcgta ctccactttg gcccactctg cccacaaatt gattccagaa gaatactcta
     3901 ccatttctga aatatgcgcg ccgcctactc ggcaggatgt ctccaaaaag taaaatttcc
     3961 catcctcgtg gcaacgaatg aactccgtat gcgaagcact gtatcgcatc ccgaaagcgt
     4021 ccatcacttg cgaggtaagt tgctgcaatg ctttnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gtcggaggaa agcgcatcca
     4201 cgtggtacac gtcgccgggt ttgaactgct caatcaaaaa caaatgccgc tcagaaccta
     4261 tttcatgcaa tacctgccag gcatcgtgcg catggtgtac ctttttgatg cctatggccg
     4321 aagcctcaga gcgcggtttg ataacccagg gaccaggaac ggtaccgaga taatgattca
     4381 cctcctcatc gttaaaaact gcgctgaatg cgggcaccgg aatgccggct tcctgagcgc
     4441 gcatacgcat ggccaatttg tcgcggaagt acttggccgt cgtctgtccc ataccgggaa
     4501 tgcgaaactc ctcgcgcagg aaagcagctt tttctacatc aaaatcatct aatgatacta
     4561 cccggtctat cttgcgggaa cgcattacat acgccaatcc ttcccggagc atgtccatgt
     4621 tctcaggcga gttgttgtcg tgttcgagat agaaaaattc gtctatactt tcgcggggcc
     4681 atgcctcatg ttccagcgat tttttggtaa taaccaacac attggcgccc agcattttgg
     4741 cggttcggat gaagtctgct cctttaaaaa aagtagctac gcaaagaaaa gtaaacggtt
     4801 tggacatggc tctaagcgat tttttatgat ggagaaaaag aggaaaaata ttccaacaaa
     4861 aggcggacgc cgtaggcagt ggcacttttt tgagaactga attcagagtc ttgcagcgca
     4921 acgccggcaa tatccaagtg cgcccagcga ggatgattgt ctataaaaac ctccaaaaac
     4981 ttggctgccg aaatagcccc cgccaccggg cgtatgccca gattgcgcac atcggctaca
     5041 tccgacttga tttcatcttt ataagcatcc cacaacggaa aacgccagag ccgttcgccg
     5101 catcgttcgc cggcctgcat gagttcgttg gccagcgcat cgtcattgct caacaacccg
     5161 cctgcatggt aacccatagc ccgaacggta gccccggtga gcgtggccaa gtctatcaac
     5221 acgtctggtt tgtaatgctt gacggcataa aataacccat cggctaaaac taagcggccc
     5281 tctgcatctg tatcaatgac ttcaatggtt ttgccgctgt atgagccgat cacatcgctg
     5341 gggcgcatgg agtgagcatc cacgctgttt tcagtggcgg gcactactcc gatgatttgc
     5401 acaggaagtt tcaacctcgc ggccgcttct actgctccca taacggctgc cgcccctccc
     5461 atgtcgctct tcataagata catattggcg gagggtttga tggaaaggcc gccggtatca
     5521 aaggtgacgc ctttgccaac caacgcaatt tttgctaccg gtttttccgg acaatattgc
     5581 gccacgatga gacatggcgg atgcgcgctt ccctgattta cagcgagtaa cgcatgaaaa
     5641 ccgagcgctt gcaattgatt tttgcccagt atgtccaccg tataattgta ccactgcgcc
     5701 gattcttccg cccaggcagc catatcaccc ggcaactttt tgttggcggg cgcattcacc
     5761 agatcaaata tgcggctttg cgtgtcggca acgattctnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     5881 agggtgttct tcgccattgc tcgttttaaa ttttccaatc cggtatgcac tgagcgccag
     5941 accattaacg gcggcttcta ccacattttc cggcttatcg ggcatgttgg cgtacaaaaa
     6001 actcactgca agatcggaag acagtctttg gcgaaatttg tggccaaagc tgcgaaatgc
     6061 tttcaatact tcgggaaaac cgggattggc gcccaagccc agcaaaaaca aacgctgttc
     6121 gcccttgtac atgggccata cttcgttcca gtcggcttta aattgcccag gttcaggaat
     6181 gccggtaacg ttttcacgac ggcataagtc caatacctcg ctcaggttgt ggtgttgcgt
     6241 tacgggtata acgaccacat attcatggtg gtcaggttgg tgggtgatgg caatctccat
     6301 gcgctctcta atgctttcaa ctaagtaata aacaataccg gaaatgctgc aagttatgca
     6361 gatttccgac ttcgacaaat atcactgaaa gaaaagccat tcgaccgctt tgggaaactc
     6421 ctctccccag cgggcttcgt tgtgtcctcc tttggggtca atggctaagt taaagtccac
     6481 gttggcagaa aaaccctgct tttccatggc ggctacaaac cgtttgatgt tgggtactac
     6541 attggcgccc tcattttcac cgccatataa atatatgcgg gaagcaaatt gctcggtgaa
     6601 cgtaagcgcc tgaaaatgaa tattgggcac tacccacaat gaaggcgaaa aaatcatcag
     6661 gcggctatac gcctcagggt acataagacc tgcgtaaatg ctgataagac ctcccataga
     6721 actgccaccg ataccggtgt gttcgcggtc gggcaaggtt ctgaattcct tatccacaaa
     6781 aggcttgagt gtgtcggcca aaaaacggac atattttttg ccgtcgccgc tgccaagacg
     6841 cgtacgcata gagggcgtaa actcggcaat acgctcactt tcagcatgat ctattgaaac
     6901 aacaatgata tcgccttgcc ccttttcgct catcatggct aattttttgt ccactgccca
     6961 attgccgaaa ggcgcataat catcaaacaa attctgcccg tcctgcaaat agagtaccgg
     7021 ataccttcgg tcggtttcgt aataatcatg cggcagcaac gcagcgatcc gacgggtttt
     7081 aatgagttgc ggaatttcaa atgcctcgtc aatcaccttg atacggggca ataattccgg
     7141 cttgtaatac agcgcctgac tgcgccaacg cggcacctgc acggcaatgc gcccggcctg
     7201 cggattggct tttctgttgt gaggcgtttt gccgaattca tccaactcct cgttttccnn
     7261 nnnnnnnnnn nnnnnnnnaa atttatactc caccaaatca ggcaaatgca agtctggcgg
     7321 aaaagagaat tcatacttac cgtgttccag cttgcgcatg agaaaacgtt tgtcgggaac
     7381 gagccaatta ttgaaattgc ccgtgaggaa tactggtaat tcactgtctt cggatgttga
     7441 cagttcgatg gtcaggttgg cttgcctttg agttgtactt tctatcatat cgctccgttc
     7501 gttcgtcatc cgtcgctatc ggtcatcggg cggcaatcgc gacgcaaaga tagcaatctt
     7561 agattaaaaa aacgggattg cttcctgtaa atccatcaca atctattttg at
//
LOCUS       DAORLF010000222         7597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5230_length_7597_cov_2.317953, whole genome shotgun sequence.
ACCESSION   DAORLF010000222 DAORLF010000000
VERSION     DAORLF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_7597_cov_2.317953"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..931
                     /gene="upp"
                     /locus_tag="PK198_16385"
     CDS             281..931
                     /gene="upp"
                     /locus_tag="PK198_16385"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRF40372.1"
                     /translation="MIHNLSDQPTIANQFLAELRDVRIQSDRMRFRRNLERIGEVLAY
                     EISRTLPYETRQVETPLGIANVALPARRVVLACILRAGLPLHEGMLHYFDHAGNAFVS
                     AYRCHHKDGSFEIKLQYVTCPDLEGCALILIDPMLATGASMVLAAQELQQFGTPAEIH
                     FASVLACRPGVDYVRRMFPKAHIWTAAIDEELTAKSYIVPGLGDAGDLAYGAKLQE"
     gene            990..2648
                     /gene="recN"
                     /locus_tag="PK198_16390"
     CDS             990..2648
                     /gene="recN"
                     /locus_tag="PK198_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRF40373.1"
                     /translation="MIKRLLIRNYAIIDELEMDFSTGLTIITGETGAGKSILLGALGL
                     VMGQRADTKALYRQDEKCVVEAVFDVSHYDLREFFEENDLDYDPELVIRREISPAGKS
                     RAFVNDTPATLDVLQGFSSALIDLHQQFDTRDIHNVSFQLRLLDALAENKPLLAEYQA
                     AFKIWQTARRRLTELERLNADSAREAEFLRFHLEEFHAAALRDGEQEELEQELALLSN
                     AEEIKRTLTAACHYLLESDQAVITQLREVGISLNTFRRINPNIGALAERYETATSEIQ
                     DIARELERLADATEYDPARLTEVQQRLDLIYRLQNKHRVSTVMELLTLQEDWESKLKN
                     LSGLDDEIAALQQQIATQREQLSAQALRLRERRQSVIAGFEQEVKAMLGQLSMEHAVL
                     QVQCAPMSELSATGMDEVNFLFAANKGGRLQLIKDVASGGELSRLTLVTKSLVAAAIP
                     LPTLIFDEIDSGVSGDVALRMGNILRRLSNQHQVVAITHAPQVAAKADAHYFVYKKDT
                     PERTLTNVRLLNEDERVRSIAVMLSQNPPSQSALQNARELLLID"
     gene            2676..3485
                     /locus_tag="PK198_16395"
     CDS             2676..3485
                     /locus_tag="PK198_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF40374.1"
                     /translation="MANNLLQGKKGIIFGALDDKSIAWKVAERCHEEGAQIVLTNAPV
                     ALRFGQINELAAKIGAEIIPADATSMEDMENLFRRSMEIFGGPCDFVLHSIGMSLNVR
                     KGKSYTDLNHEWMLKTFDISAMSFHRMMQVMMKLDAISEGGSILALSYIAAQRVFPDY
                     NEMAESKALLESFARSFGYHFGTQKKVRVNTISQSPTKTTAGTGVKGFMEFFDYADKM
                     SPLGNASSEACADYCVVMFSDLTRMVTMQNLFHDGGFSNMGMNPAVFGIEG"
     gene            3945..4982
                     /locus_tag="PK198_16400"
     CDS             3945..4982
                     /locus_tag="PK198_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40375.1"
                     /translation="MSLRILIVCLLCSHLLSGQVVVEYAVRIEEEVSLLQRATLPQRV
                     LIATNGNRVVVRGFWETPRNSYQLYVPEEQVFYHCDLESDIAVRYKAEPSGPVWLVSG
                     STATLAGLACKEAFTVIEADTFPIFYTDAFGVQFCQVAPVPGFAMRYTKRLQGIKVTY
                     EAVKYHFEMVPEGMFSLIGKKVFDSDNPADGRQWAMQIGRKAPKIKTACLDGSQLTPK
                     SYAGKVLVLDFNSLGWGGFNVNDLNWLDTYAKEYADHPNVMFVSVFRESEDNLRRLLP
                     LLRPGYKVIADGQFYNDAFNIELLPATIVINRYGRIAEYVAGRTPLSEARLRRAIDLA
                     LSGGLKPAGLE"
     assembly_gap    5172..5193
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5194..6646
                     /locus_tag="PK198_16405"
     CDS             <5194..6646
                     /locus_tag="PK198_16405"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40376.1"
                     /translation="LVSLFRHLFLGNRDVFKDLYIGLHTDYDFVEYPVLQFNFADFGL
                     KTKNLEEELELVIQGYAAEFGVDIETSSIATQFRTLIQNISRSRGKPVALLIDEYDKP
                     IIDFLTEVDKAEANQQILRQLFGPLKGLEAAGHLRFLFITGVSKFSKVSLFSDLNNLT
                     DLSIHSLSHDLLGITQAELTYYFGEYMDQAASKYELTREGLTEAIKNWYNGYSYDGKT
                     RLHNPFSLLQFFQNQQFGNYWFATGTPTFLVETIRNRGIHPQEFEAVVLDENFLNRFS
                     LLDLNMTGLLFQTGYLTIQQVEASIYETTYHLGYPNYEVRHSMMHNMVEAFTYQPASL
                     VSQSLIEMRNGLRQGNIHLFMEALRVILSDLKYNWQPPKQYKSEDELLKMWEGYFHAV
                     LYLITAYMDMSVRAELAHHKGRIDLVATTNDFVYLMEFKMESSARAALAQIKDREYAL
                     PFKNSPQRVFLVGINFSNEERNLESWEAEEWKD"
     gene            complement(6823..7461)
                     /locus_tag="PK198_16410"
     CDS             complement(6823..7461)
                     /locus_tag="PK198_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HRF40377.1"
                     /translation="MKSSLNERLLACCTEMQGEFGLIPEVRQQELAELGAYIAQKIQS
                     GQAAQLTVICTHNSRRSNIGQLWLAAAAAYYGIEGLAAFSGGTEATAFNPRAVAAMRR
                     AGFDISGPEAEANPRYTALMGAGLPELVLFSKRYDDPENPQQSFAAIMVCSDADEACP
                     FIPGAEKRFAIRYDDPKASDGTPQEAATYDERCRQIGREMLFAMWCVAEQGK"
BASE COUNT         1737 a         2179 c         1921 g         1738 t
ORIGIN      
        1 cattattgta gtaaaaaaag gcgccacgat tgccaatggt acgcgcatag ggctgggggc
       61 ataacattta tggtgtcgta cctatacagt cgtctgctaa aagctgcgag ccgcgagtag
      121 cgagccgcga gtttgatttt ccaagataaa aaagccaatc attttcaata gtagattttg
      181 aaatttcccg tatggaaaat ctaagttttc aaccagcaac cagactcgtg gctcatagct
      241 cgtagctcgt agctcttgga gatacactga ccatctatct atgattcaca acctgagcga
      301 tcaacctacc attgccaatc agtttttggc cgaactccgc gatgtgcgca tccagagcga
      361 ccgcatgcgt ttccggcgca atctggagcg cattggggag gtactggctt atgaaatcag
      421 ccggacgctg ccctacgaaa cgcgtcaggt agaaactccg ctgggtattg ccaatgtggc
      481 cctgccggct cggcgtgtag tgctggcttg cattttgagg gcgggcctgc cgctacatga
      541 aggcatgttg cattacttcg atcatgcggg caatgctttt gtgtcggcct atcgttgcca
      601 ccacaaagac ggcagttttg agattaaact gcaatatgtg acctgccccg atctggaagg
      661 ctgtgcgctc attctcattg acccgatgct ggccaccggc gcttctatgg tgctggcagc
      721 gcaggaattg caacagttcg gcacgcctgc cgaaattcat ttcgcctctg tgttggcctg
      781 ccgccctggc gtggactatg tgcggcgcat gtttcccaaa gcgcatatct ggacggcagc
      841 catagacgaa gaactgacgg ccaaatcgta catcgtgccc ggcctgggcg atgccggcga
      901 tctggcctac ggcgcgaagt tgcaggagtg atgctacaaa taatcgtcac attgcctatt
      961 tttgcccccg cttttgcgaa cgtacgccta tgatcaaaag actcctcatt cggaattacg
     1021 ccatcattga cgagctggaa atggattttt ccaccggcct caccattatt acgggcgaaa
     1081 ccggcgcagg aaaatccatc ctgttgggcg ccctcggctt ggtcatgggg cagcgtgccg
     1141 acaccaaagc cctataccgg caagacgaaa agtgcgtggt ggaagccgtt tttgatgtga
     1201 gccactacga ccttcgggag ttttttgaag aaaacgacct cgactacgac cccgaattgg
     1261 tcatccgccg cgaaatatcg cctgccggca aatcccgcgc ttttgtcaac gacacccccg
     1321 ctacgcttga tgtcttgcag ggattcagca gcgccctcat tgatctgcac cagcaattcg
     1381 atacccgcga catacacaat gtatccttcc aactgcgcct gctcgatgca ctggccgaaa
     1441 acaagcctct gctggccgag tatcaggccg ccttcaaaat atggcagacc gcccgtcgcc
     1501 gcttgaccga actcgaacgc ctcaacgccg acagcgcccg cgaagccgag ttcctgcgtt
     1561 tccacctcga agagttccac gctgccgccc tccgcgacgg cgaacaggaa gaactcgaac
     1621 aggaactcgc cctgctctcc aatgccgaag aaatcaagcg caccctcacc gccgcctgcc
     1681 attatttgct ggaaagcgat caggccgtca tcactcaatt gcgcgaggtg ggcatttcgc
     1741 tcaacacctt ccgccgcatc aatcccaaca tcggcgcgct ggccgagcgc tacgaaacgg
     1801 ccacttctga aattcaggac attgcccgcg aattggagcg cctggccgat gccaccgaat
     1861 acgatcctgc ccgcctgaca gaagtgcagc agcggctcga tctcatctac cgcctgcaaa
     1921 acaagcaccg cgtatccacc gtgatggagc tgctgacttt gcaagaggat tgggaaagca
     1981 aactgaaaaa tctctccggt ttggatgatg aaatcgccgc tttgcaacag caaatcgcaa
     2041 cgcagcgcga gcaactcagc gcccaggccc tccgcctgcg cgagcgccgc cagtcggtca
     2101 ttgccggttt tgaacaggaa gtcaaggcca tgctcggcca actttcgatg gaacacgccg
     2161 ttttgcaggt gcaatgtgcc cccatgtccg agctgtcggc taccggcatg gacgaggtca
     2221 attttctctt tgcggccaat aagggcgggc gtctccaact catcaaagac gtggcctccg
     2281 gcggcgagct ttcccgcctt acgctcgtca ccaaatcttt ggtagctgcg gccattcctt
     2341 tacccacgct cattttcgac gagatagatt ccggcgtttc cggcgacgtg gccctgcgca
     2401 tgggcaacat cctgcgccgc ctctccaacc agcatcaggt ggtggccatc acccacgcgc
     2461 cgcaggtagc cgccaaagcc gacgcacatt attttgttta taaaaaggac acgcccgaac
     2521 gcacccttac caacgtgcgc ctgctcaacg aagacgagcg cgtccgctct atcgccgtca
     2581 tgctcagcca gaatccgccc agccagtcgg cactgcaaaa tgcccgcgag ctgctgctta
     2641 ttgattgatt tttctgcatc aacaaccata acaacatggc aaacaatctc ttacaaggta
     2701 aaaaaggcat catctttggt gcgctcgacg acaaatccat cgcctggaaa gtagccgaac
     2761 gctgccacga agaaggcgct cagatcgtgc tcaccaacgc gccggtagcc ctgcgttttg
     2821 gtcagatcaa cgaactggcc gccaaaatcg gcgccgaaat catacccgcc gacgccacca
     2881 gcatggagga catggaaaac ctcttccgcc gctctatgga aatcttcggc ggcccctgcg
     2941 acttcgtgct gcactccatc ggcatgtccc tcaacgtgcg caaaggcaag tcctacaccg
     3001 acctcaacca cgagtggatg ctcaaaacct tcgacatttc ggccatgtcc ttccaccgca
     3061 tgatgcaggt gatgatgaaa ttggacgcca tctccgaagg cggttccatc ctcgccctgt
     3121 cgtacattgc cgcccaacgc gtcttccccg actacaacga aatggccgag tcgaaagccc
     3181 tgctcgagtc cttcgcccgc agcttcggct accacttcgg cacccaaaag aaagtgcgcg
     3241 tcaacacgat ttcgcagtcg cctaccaaaa ccactgccgg caccggcgtc aaaggtttta
     3301 tggagttctt cgactacgcc gacaagatgt cgccgctggg caatgcctcc tccgaggcct
     3361 gcgccgacta ctgcgtggtc atgttttccg atctcacccg catggtcacc atgcaaaatc
     3421 tcttccacga cggcggtttc tccaacatgg gcatgaaccc cgctgtgttc ggcatcgagg
     3481 gctgataatc gcccctctac ttaggtgttc ttacgacgtt tgccgggctt ggaatagtcg
     3541 ggcaaacgtc gtttttttat ggcagccgcc cgctcgtcgc cagagctgtt agctttaccc
     3601 ccgcaataac cggccaaagc taacagatat cttcctgctg cgggcgccgc gtgctttgct
     3661 cttacctcct ccaggcgata taaaataaat tttcattttt gtcaaaggat taactaagta
     3721 aacgcttcat tttaagtgtc ttagctaatt ctttggcctg aagaaaatga aaatttattt
     3781 tcgccccacc gtcggtaccg cttcgcccgc tgctgcaagg ccctgctccc ggtagttgtc
     3841 agccgcccca tatctgccct tcgccctgat tttcgccaca cccccgtcaa caactcgatc
     3901 aatagatcgt taagcataaa cccacccgac acatctgcat tgctatgagt ctgcgtatcc
     3961 tgattgtttg tctgttgtgt tcccatctgc tgtccggaca ggtggtggtg gagtatgccg
     4021 ttcgcatcga agaagaggtc agcctcctgc aacgcgccac cctgccgcag cgcgtactca
     4081 tagccaccaa cggcaatcgg gtggtggtgc gcggattttg ggaaacgccc cgcaacagtt
     4141 accaactcta tgtgcccgaa gaacaggtat tttatcactg cgatctggag agcgatattg
     4201 ccgtgcgcta caaggccgag ccgagtggcc cggtctggct ggtttccggc tcaacggcta
     4261 cccttgccgg actggcgtgt aaagaggctt tcaccgtcat agaggcggat accttcccca
     4321 ttttttacac cgatgctttc ggagttcagt tttgccaggt ggcgcctgtg cccggttttg
     4381 ccatgcgcta caccaagcgt ttgcagggca tcaaagtgac ttatgaagcc gtgaagtacc
     4441 actttgaaat ggtgccagaa ggcatgtttt ccctcattgg caaaaaagta ttcgacagcg
     4501 ataaccccgc cgacggtcgg cagtgggcca tgcaaattgg ccgaaaggcc cccaaaataa
     4561 agaccgcctg tctggacggc tcccaattga cccccaaatc ttacgcgggc aaagtattgg
     4621 tgctcgattt caacagcttg ggctggggcg gtttcaatgt caacgatctc aactggttgg
     4681 atacctacgc caaagaatac gccgaccacc ccaatgtaat gttcgtttcg gttttccgcg
     4741 aatcggaaga caacctgcgc cgcctgttgc cgctcttgcg ccccggctac aaggtcattg
     4801 ccgacggcca gttttacaac gacgctttca acatcgaact cctgccggcc accatcgtca
     4861 tcaatcggta cggccgcatt gccgagtatg tggcaggccg cacgcctttg tcggaggccc
     4921 gcctgcgccg cgccattgat ctggcgttga gcggcggcct gaaaccggcg gggttggagt
     4981 gagggggcat aacgagaatt aaagcatatc tttgccatgc gtatgagaca aagcgtatga
     5041 agttattacc aacaggaagg caaaattttg aagacgtcat caaggaaaac ttgttgtatg
     5101 tggacaaaac ccggcaggtg tttgaactac tgcggcaggg gcggctgtat tttctctctc
     5161 gcccgcgcag gnnnnnnnnn nnnnnnnnnn nnngttggta tcattgtttc ggcatttgtt
     5221 tttgggcaac cgagacgtgt tcaaggattt gtacatcggc cttcataccg actatgactt
     5281 tgttgagtac cccgtgctgc aattcaattt tgcggatttt ggtttgaaaa caaagaatct
     5341 ggaagaagaa ttggaattgg tgatacaggg ctatgccgcc gaatttggcg ttgatattga
     5401 aaccagctcc atcgccacac aattcagaac gcttattcaa aatatttccc gctcaagggg
     5461 caagcccgtg gcgctgctca ttgacgagta cgacaaaccc attattgatt ttctcacgga
     5521 agtggataag gccgaggcca atcaacagat tctcaggcaa ttgttcggtc cgctcaaagg
     5581 attggaagcg gcgggacatc tccggtttct gttcattacg ggcgtgtcca aattcagcaa
     5641 ggtcagcctt ttttcagatt tgaacaacct caccgatttg agcatccatt cattgagcca
     5701 tgatctgctg ggcattacac aggcggagtt gacctattat ttcggcgaat acatggatca
     5761 ggcggcatca aaatatgaac tcacccgcga aggattgacc gaggccatca aaaactggta
     5821 caatggttac tcttatgacg gtaaaacccg cctgcacaat ccattttctt tgctgcagtt
     5881 ttttcaaaac cagcagttcg gcaactattg gtttgccaca ggtacaccga cttttttagt
     5941 agaaaccatt cgcaaccggg gcattcaccc gcaggagttc gaggcggtcg ttttagacga
     6001 aaacttcctc aaccgctttt ctctgctcga tctcaatatg acagggctgc tcttccaaac
     6061 gggctatctc accattcagc aggtggaggc ctccatctac gagactacct accacttggg
     6121 ctatcccaac tacgaggtgc ggcactctat gatgcacaac atggtggagg cattcactta
     6181 tcagccggct tcattggtca gtcaatcgct tatagagatg cgaaacggcc tgcggcaagg
     6241 caatattcat ctctttatgg aggcgctcag agtgatactg tctgatctga agtacaattg
     6301 gcaaccgccg aaacaataca aatcagagga cgagctgctg aaaatgtggg agggctattt
     6361 tcacgcggta ttgtacctca tcacggcata catggacatg tcggttcggg ccgaattggc
     6421 gcaccacaaa ggccggattg atctggtggc cactacaaat gattttgttt accttatgga
     6481 atttaagatg gagagcagcg ccagggccgc tctggcacag ataaaggaca gggagtatgc
     6541 cttgcctttt aaaaacagcc cgcaaagggt gtttttggtg ggcatcaatt tctcgaacga
     6601 agaacgcaat ttggaaagtt gggaagcgga agagtggaaa gattgagaat acaggaaacg
     6661 ccgccgctgc aaccagccca atgaggcgat actttattct attccccgta ccctcgatac
     6721 aaagcgactt gtacaactca tttttcacct cctcccggcg caggataata ccgtcggcgc
     6781 aaccaaaagg ccacattgac cagggcaatc agcgccggca cctcatttac cctgctctgc
     6841 tacacaccac atcgcgaaaa gcatttcgcg cccgatttgc cggcagcgtt cgtcgtaggt
     6901 agccgcctct tggggcgttc cgtcggaggc tttcgggtcg tcgtagcgaa tggcaaaacg
     6961 tttttcggcg cccggaataa aagggcaggc ttcgtcggca tcagaacaaa ccatgatggc
     7021 ggcaaagctc tgctgcgggt tctccggatc atcgtacctt ttggaaaaca gcaccaactc
     7081 cggtagcccc gctcccatga gagccgtgta tcgggggttg gcctctgcct ccggccccga
     7141 aatgtcgaaa cccgcccggc gcatggccgc tacggcgcgg gggttgaaag cagtggcctc
     7201 tgtgccgccg gaaaaggcag ccagaccctc tattccgtaa taagcggcag cggccgccag
     7261 ccagagttgt ccgatattgc tgcgccggga gttgtgagtg cagatgacag tgagttgtgc
     7321 cgcttgcccg gattgtattt tttgagcaat ataggccccc agttcggcca attcctgctg
     7381 cctgacttcg ggtatgaggc cgaattcgcc ctgcatttcg gtgcaacagg ccagcagccg
     7441 ctcgtttagt gatgatttca ttgatatgga agatggttag aacaatcccc ctcagcaaca
     7501 cccgctgccc ggctcgcagc agttggcttt tcccagttcg gagaggtgca gcatcggttt
     7561 ccttttcctt ttttcgcccg gtttttcggc atacacg
//
LOCUS       DAORLF010000223         7575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5259_length_7575_cov_2.257846, whole genome shotgun sequence.
ACCESSION   DAORLF010000223 DAORLF010000000
VERSION     DAORLF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_7575_cov_2.257846"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK198_16415"
     CDS             complement(<1..590)
                     /locus_tag="PK198_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRF40378.1"
                     /translation="MHHKTTIEPFRIKVVEQIRLSSEEERLQYLADAQYNPFLLKSNQ
                     VIIDLLTDSGTSAMSSEQWAGVMRGDESYAGAVSWQRLENAVKDLTGYPYVMPTHQGR
                     AAEHLLYSRIGGPGKVFISNTHFDTTRANIEFSGATAIDIPIEEGRHPDWDHPFKGNM
                     DTARLEALIAEHGAANIGAVVLTVTNNSGGGQPVSMA"
     gene            complement(615..1850)
                     /locus_tag="PK198_16420"
     CDS             complement(615..1850)
                     /locus_tag="PK198_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRF40379.1"
                     /translation="MELTLILIFLAFSALFSGLEIAFVTSNKLKVELRKKKGAHRSQI
                     LAGFYENPSVFIGSMLVGNNIVMVGLTYFATQLLNPYLQPFVGDGFLLMLANTILITM
                     VVLMFGEFLPKTIFRLYADQILYLLAYPVQVLLWLLSPVAWLFNKLSLLIIRLVMRTE
                     TVVSDPVFTRLDLERFIMESGSDAEGEVVDREMFGNALNLKEVRVRDCMVPRPEIECI
                     DINAGVEELEQLFRETKLSRILVIEDDVDNVLGYVHHQQLLERPSNIDKIRLEIIFVP
                     EVMRVSDLLHKFITERVNIVCVVDEFGGVAGLITLEDILEEIFGEIEDEHDEEEYIEQ
                     QISETEYLLSGRLEIDYLNEKFPSLHLPEGEYHTLSGYLVMTTGSIPQQGAEIELNGT
                     RFILEQVSETKIETVRILK"
     gene            complement(1850..2416)
                     /gene="lptC"
                     /locus_tag="PK198_16425"
     CDS             complement(1850..2416)
                     /gene="lptC"
                     /locus_tag="PK198_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRF40380.1"
                     /translation="MKSITFIIIAAILLAGCREEAPETLPDALLSNPNMELLEEVEIL
                     YSDSAALKVRVTGPVMHYFTEANDPRQEFPCGVIVHFLDRDQQVASILTAKYAIRRES
                     IGTIIVRDSVVWQSVRKEHLTTDELTWDERRQKVFTDRFVVVTRPGEILYGRGFEANQ
                     DFSNLTMKAVEGRVVVDEEKNENQAENN"
     assembly_gap    2549..2558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2559..5444)
                     /locus_tag="PK198_16430"
     CDS             complement(<2559..5444)
                     /locus_tag="PK198_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40381.1"
                     /translation="MRKQVLLRSLLLISSFVFQMAVLQAQPANDECINAIALNNVSGW
                     CSTNGQFTTVGATPSVQTNPNCFPNNTASNDVWFSFVTQANTVNISVAGTTTINAGGT
                     ISNPQFALYSGTCGNLTLVQCASDAMNDHIIQTFAGDLVIGQIYYIRVSARFAFRGTF
                     RLCVNNYNEVPSPSGDCGTAVVLCDKSSFTVPKVVGTGSQNNEIGNVGCNGPSCVITE
                     SSSTWYKWTCRDAGTLTFTLTPLNPADDLDFVVYELPGGLDNCNNKNPIRCMASGENV
                     SQPIATWLPCTGATGLRNGETDTNETCGCPSGNNNFIAPINMVAGRSYALVVNNFSNS
                     GDGFTVTFGGTGTFLGPTADFSISPQTVCVGQPVTLTDQSTFTGVITDRTWSFGLNST
                     PTTATGAGPHTVSFDRPGIKSVLLTVRTDRGCLVSKVLTVNVECCPDHFTSNQNITDV
                     LCAGASTGAIDVSIASNYPPYTYTWGGGQTSQDISGLPAGSYALTVTDRATCSRELNY
                     IVTQPTPVETDTIITMPTCNGGVDGAATLQPRGGVGPYQFNWQGTGFSSNNTLANIPR
                     GDYPVVIRDANNCLLPLVIPVRELELILNPTVQAITQPTCTGFSDGSIVVDIANGRGP
                     FQYNWGAGFVPDNSLLNVAAGVYNVEVRDANLCLGIFQFDMQDHPPLALGFDPTNTLC
                     FQDANGSAVVTPSGGVGNYTYAWQTGSLTNTATGLSAGTYTVTVLDGNNCRIEGAVTI
                     TEPPLLQLIVTDSTGLLCSYDTTGRIGVMAIGGTPDYEYSVDGNVFQMAPVFNSLRGG
                     TYTFTVMDAQGCTATVAGTVNAPPPIVVDAGPNQIIELAYTADISATVSQNNLTISWT
                     PPISLDCPTCLSNTVAPLQNTTYVLTALDPNNCIAIDSVTILVVKNRPIYIPNAISPN
                     DDGINDGFTLYAGPAARQINRLRIFDRWGELIFDKSNIPLGVENQGWD"
     gene            5547..6038
                     /locus_tag="PK198_16435"
     CDS             5547..6038
                     /locus_tag="PK198_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRF40382.1"
                     /translation="MKKHLPNAITLLNLFFGCMALTAVFRGDVYAVAAFTAAAALADF
                     LDGALARLLHVHSPLGAQLDSLADAVSFGLVPGAVFFYLLGGTQTDGFSWEAAPGFVL
                     TLFAVLRLGRFNLDTRQTEHFIGLPTPAATIFVLGWLLAVHTDALGWGAALAERNLFM
                     QPL"
     gene            6330..6998
                     /gene="rsmI"
                     /locus_tag="PK198_16440"
     CDS             6330..6998
                     /gene="rsmI"
                     /locus_tag="PK198_16440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRF40383.1"
                     /translation="MLHVVPTPIGNLEDITLRAIRILKEADLVLAEDTRTTKVLFQKH
                     GIETPLRAFHAHNEHGVTQGIVAQLQTGASIALVSDAGTPGISDPGFLLIRACVQGGV
                     QVECLPGATAFVPALVASGLPCDRFHYEGFLPHKKGRQTRLKYLATLPNTFVLYESPH
                     RLLRCIEELMAVCGPERRACVARELSKLHEELHRGSLDELRAYFSDASKVRGEIAIVV
                     AGNE"
     gene            complement(7008..>7575)
                     /locus_tag="PK198_16445"
     CDS             complement(7008..>7575)
                     /locus_tag="PK198_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF40384.1"
                     /translation="LGIRSSDTHSIMYNDVKVPKSNRIGEDGFGFKFAMKTLSGGRIG
                     IASQALGIAGGAFELATAYSKERQAFGKPISQHQAIAFKLADMATEIEAAKLMVYRAA
                     WLKDKKMDYTAAGAMAKLYASEVAMRHTVEAVQIHGGYGFVKEYHVERLMRDAKITQI
                     YEGTSEVQRIVIARNVLDGNLYLPITGA"
BASE COUNT         1674 a         1969 c         2103 g         1819 t
ORIGIN      
        1 gccatgctca caggctgacc gccgccgctg ttgttggtaa cagtaagtac taccgcgccg
       61 atgttggcag ccccatgttc ggcgatgagc gcttccaaac gagccgtgtc catattacct
      121 ttaaaggggt gatcccagtc ggggtggcgg ccttcttcaa tcggaatgtc tatggcagta
      181 gcgccggaaa actcaatatt ggcgcgggtg gtatcgaaat gggtgttgga aataaacacc
      241 ttgcccggcc cgccgatgcg actgtacagg aggtgctcgg cggcgcgacc ctgatgggtg
      301 ggcatcacat aagggtagcc ggtgaggtct ttgacggcgt tttcgaggcg ctgccaactg
      361 actgcgccgg catagctttc gtcgccgcgc atgacgccgg cccattgctc ggaactcatg
      421 gccgaagtgc cgctgtcggt gagcaagtca atgatgacct gatttgattt gagcagaaaa
      481 ggattgtatt gcgcgtctgc taagtattgg aggcgctcct cttcggaact gagccgtatt
      541 tgttccacaa ccttgatgcg gaacggctcg atggtggttt tgtgatgcat ggtgttggct
      601 ttatttggcc gtgcttattt caaaatgcgc acggtttcta ttttcgtctc gctgacctgc
      661 tccaaaatga agcgggttcc gttgagttct atctcggcgc cctgctgagg gatgctgccg
      721 gtagtcatta ccaaatagcc ggataatgtg tggtattcgc cttccggcag atgcaaagag
      781 gggaattttt cattgagata atcaatttcg aggcggccgg agaggaggta ttccgtctcg
      841 ctgatctgct gctcgatgta ttcttcctca tcgtgttcgt cttctatctc gccgaaaatt
      901 tcttccaaaa tatcctccag cgtaataaga ccggccactc cgccgaattc gtccaccaca
      961 cacacgatat tgacccgctc tgtaatgaat ttgtgcagca aatcggaaac gcgcatcacc
     1021 tccggcacaa aaatgatttc gaggcggatt ttgtcaatat tggacggccg ctccagcagt
     1081 tgttgatgat gcacatagcc cagcacattg tccacatcgt cttcaatgac caaaatgcgc
     1141 gagagttttg tttcacggaa caattgctcc aattcttcca ctccggcgtt gatgtcaatg
     1201 cattcaattt cggggcgggg caccatgcaa tcgcgcactc gtacctcttt gagattgagc
     1261 gcattgccga acatttcccg atccactacc tctccttctg catcgctgcc gctttccata
     1321 atgaatcgct ccagatcgag gcgggtgaaa acaggatcgc ttactaccgt ttccgtacgc
     1381 atcaccaagc ggatgatgag taaagacagt ttgttgaaca accaggccac cggcgacaac
     1441 agccacagca acacctgcac ggggtatgcc agcagataca atatctggtc ggcatacaat
     1501 ctgaaaatgg ttttgggcag gaattcgccg aacatcaata ccaccatggt aatgagaatg
     1561 gtattggcca acatgagcag gaacccgtcg ccgacaaatg gctgaaggta aggattgagc
     1621 agttgggtgg caaaataagt cagccccacc atcacaatgt tgttgcccac cagcatagag
     1681 ccgatgaaaa cagaggggtt ttcataaaag ccggccaata tctggctgcg atgggcgcct
     1741 ttttttttgc gcagctctac tttgagttta ttggatgtca cgaacgcaat ctccaggccg
     1801 gaaaaaagtg ccgaaaaggc cagaaaaatg agaatcagtg tgagttccat cagttgtttt
     1861 cggcttggtt ttcgtttttt tcttcatcca ccaccacgcg gccttccacc gctttcatgg
     1921 tgaggttgct aaagtcttga ttggcctcaa agccccgccc gtaaaggatt tctccgggcc
     1981 gggtgacgac gacaaagcga tctgtgaaca ctttttggcg gcgctcgtcc caggtgagtt
     2041 cgtccgtagt gagatgctcc ttgcgcacgc tttgccacac cacgctgtcg cgcacaatga
     2101 tggtgccgat gctctcgcgg cgaatggcat atttggccgt caggatgctg gccacctgtt
     2161 gatcgcgatc cagaaaatgt acaatcacgc cgcagggaaa ctcctgtcgg gggtcattgg
     2221 cctctgtaaa atagtgcatc accggaccgg tcacgcgtac tttcaacgcg gcggaatcgc
     2281 tgtacaggat ttccacttct tcgagcagct ccatgttggg attgctcaaa agcgcatcgg
     2341 gcagcgtttc gggcgcttct tcccggcagc cggccaacag tatggcagca atgataatga
     2401 atgtaattga cttcatcagg ctgcaaaggt acaatctttg ttccttattt tacgatggtc
     2461 acatcgccct cgtacagcac gttcacatcg tcaataaagc ccacctcggc cataaataca
     2521 tacactccgg cattggcagg ccgcccccnn nnnnnnnngt cccatccctg gttttcgacg
     2581 ccgagcggga tgttgctttt gtcgaaaatc aattctcccc agcggtcgaa aatgcggagg
     2641 cggttgatct gccgggctgc cggaccagcg tacaaagtaa agccgtcatt gatgccgtcg
     2701 tcgttggggc tgatggcgtt gggaatgtaa attggccggt ttttgacaac gaggatggtg
     2761 acggaatcta tggctataca attgttgggg tctaatgcgg taagcacata agtggtgttt
     2821 tgcaagggag ccaccgtatt ggacaggcaa gtagggcagt ctaatgaaat gggtggcgtc
     2881 caactaatgg tcaggttgtt ctggctcacc gtagcactga tatcggcggt gtaggccaat
     2941 tcgatgatct gattggggcc ggcgtccacg acgatgggcg gcggagcatt tacggttccg
     3001 gcaacggtgg ccgtacaacc ctgcgcatcc attacggtaa aagtgtaagt gccgccccga
     3061 agggaattga acacaggcgc catttggaaa acgttgccgt ccacgctgta ttcatagtcg
     3121 ggggtaccgc caatggccat cactcctatg cggccggtag tgtcgtagct gcacagcagg
     3181 ccggtgctgt cggtgacaat gagttgcagc agcggcggct cggtgatggt gacggcgccc
     3241 tcaatgcggc agttgttgcc atccaaaacg gtgacggtgt aggttccggc acttaatccg
     3301 gtggccgtgt tggtaagaga gcctgtttgc caggcatagg tataattgcc tactccgccg
     3361 gaaggcgtca ccacggcact gccgttggca tcctgaaaac agagggtgtt ggtgggatcg
     3421 aaaccgaggg ctaaaggcgg gtggtcttgc atatcaaatt gaaaaatacc cagacagagg
     3481 ttggcatccc gcacttccac attatacacg ccggcggcca cattgagcag cgaattgtcg
     3541 ggcacaaaac ctgctcccca attgtactga aacggccctc gtccgttggc aatgtccacc
     3601 acaatggagc catcagaaaa gcctgtacag gtaggctgcg taatggcttg caccgtggga
     3661 ttgagaatca gttccagctc ccgcaccgga atgacgagcg gcaacaagca gttgttggca
     3721 tcccgaatga cgacgggata atcgccgcga ggaatattgg ccagcgtatt gttgctgctg
     3781 aagcccgtgc cttgccagtt gaattggtac ggccctaccc cgcccctcgg ctgtagggtg
     3841 gcggctccgt caactcctcc gttgcaggtg ggcatggtga tgatcgtgtc cgtctcgaca
     3901 ggcgtcggct gggtaacgat gtaattcagt tcccggctac aggtggcccg gtcggtgacg
     3961 gtgagcgcat agctacccgc cggcagcccg ctgatgtcct gcgatgtctg gcctcctccc
     4021 caagtgtagg tataaggcgg gtaattgctc gcgatgctca cgtctatggc gccggtggaa
     4081 gctcccgcac agagtacatc ggtgatgttt tgattgcttg tgaagtgatc cgggcagcat
     4141 tccacattga cggtcaaaac tttagaaaca aggcaccccc ggtcggttcg cacggtgagg
     4201 aggacagact taatgcccgg ccggtcgaag gagacggtat gcggccctgc tccggtagcg
     4261 gtggtcgggg tcgaattcag cccgaagctc caggtgcggt cggtgatgac gcctgtgaaa
     4321 gtggattggt cggtgagcgt aactggctgc ccgacacaca ccgtctgggg gctgatgctg
     4381 aaatcggccg tcggccccaa aaaggtgccg gtaccgccaa aggtcacggt gaagccgtcg
     4441 ccggaattac tgaagttatt gacaacgagg gcatagctcc tgcctgccac catattgatg
     4501 ggggcaatga aattgttgtt gcccgatggg cacccgcagg tttcgttggt atctgtttcg
     4561 ccgttgcgca agccggtggc gccggtacag ggcagccagg tagcaatggg ttgactcacg
     4621 ttttcgcccg aagccataca gcgaatggga tttttgttgt tgcaattatc caaaccgccg
     4681 ggcaattcat acaccacaaa atcgaggtcg tcggcagggt tgagaggtgt gagggtgaag
     4741 gtgagcgtgc ctgcatcgcg gcaggtccat ttgtaccagg tagagctact ctcggtgatg
     4801 acacaggaag ggccattgca gcccacgttg ccgatttcat tgttttggct gccggttcct
     4861 actactttcg gaacagtgaa ggaggatttg tcgcacaaaa ccacggcagt tccgcagtcg
     4921 ccggaagggc tgggtacctc gttgtagttg tttacacaga gcctgaaggt gcctctgaag
     4981 gcaaacctgg cactgacgcg gatgtagtat atttggccaa tgaccagatc gccggcaaag
     5041 gtttgaatga tatgatcgtt catcgcatcc gaggcgcact gcaccaatgt caaattgccg
     5101 catgtgccgg agtaaagcgc gaattgagga ttggaaatgg tgcctcctgc attaatggtg
     5161 gtggtgccgg ccacactgat gttcactgta ttggcctgcg tcacaaacga aaaccacaca
     5221 tcattggagg cagtgttgtt gggaaagcaa ttgggattgg tctgcacaga aggcgtggcg
     5281 cccacggtgg taaactggcc gttggtagaa caccagccac tgacattgtt tagtgcaatg
     5341 gcattgatac actcgtcgtt ggcaggttga gcctgcaaaa cggccatttg gaaaacaaag
     5401 gaagaaataa ggagcaatga ccttaggagg acttgttttc tcatcagaat cggttttatt
     5461 ctttgcttta aacgataaaa ttagcaattg tatgaagtaa aagttttaag aaaaacctaa
     5521 aatgcgacat ttgtcctcta atggccatga aaaaacatct tcccaacgca attacgctgc
     5581 tcaacctgtt cttcggttgt atggcgctta ccgccgtttt tcgcggcgat gtctatgctg
     5641 tagcggcatt tacggctgct gctgcccttg ccgatttctt agacggagcg ctggcacgcc
     5701 tgctgcatgt ccactctccg cttggcgctc aattggattc cctggccgat gccgtatctt
     5761 tcggactcgt gcccggcgcg gtttttttct acctgctcgg cggcacgcaa accgatggat
     5821 tctcatggga ggctgcgccg ggctttgtac tcaccttatt tgctgttttg cgcctcggac
     5881 gcttcaattt ggacacccgc caaaccgagc atttcatcgg tttgcccacg cccgcagcta
     5941 ccatcttcgt gctgggctgg ctgttggctg ttcatacaga cgcattagga tggggggcag
     6001 cgttggcaga aaggaattta tttatgcagc cattgtagct ttgtcctggc tgatgaatgc
     6061 cgaattgccc atgttcagcc tcaaattcaa agcgtttcaa tggaagggca atgagataag
     6121 gattattttt gcggcagcgt cagtcggcct gctcgtttgg cttcagtcgg cagcgctctt
     6181 gcccattatt ctggcgtata tcgtcgtttc ggtcatttta gacagggttc attccaatcg
     6241 aaaaccatga aataccttgt agccggcagc atcatccccc ggatgaaaat gcacttctta
     6301 tcacttcggt tcatcaagat ttaacccaca tgctgcacgt tgttcccaca cccatcggca
     6361 atctggagga catcacgctc cgggccattc gcattctcaa ggaggctgat ttggtactgg
     6421 ccgaagatac gcgcaccaca aaagtattgt ttcaaaaaca cggcatcgaa acgcccctgc
     6481 gcgctttcca cgctcacaac gagcacggcg tcacgcaggg cattgtggcc caactccaga
     6541 cgggcgccag catagcactt gtttccgatg ccggtacgcc gggcatctcc gacccaggtt
     6601 ttttgctcat tcgggcttgt gtgcaaggcg gggttcaagt ggaatgcctg ccgggcgcca
     6661 cggcgtttgt tccggctttg gtagcttcgg ggttgccctg cgaccggttt cactacgagg
     6721 gctttttacc tcacaaaaag ggccggcaaa caagactgaa atacttggcc accctgccca
     6781 atacctttgt tttgtacgaa tctccgcacc ggcttttgcg ctgcatcgaa gaacttatgg
     6841 ccgtgtgcgg ccccgaacgc cgtgcctgcg tggcgcgcga actgagcaaa ctgcacgagg
     6901 aactgcatcg gggcagcctc gatgaactcc gcgcatactt cagcgacgcg agcaaagtac
     6961 gcggagagat tgcaatagtg gtagcaggaa acgagtaaaa taaaagccta cgctccggta
     7021 atgggcagat acaaattgcc gtccagcaca ttgcgggcaa tgacgatgcg ctgcacctca
     7081 gaagtaccct cgtaaatttg cgtaatcttg gcatcgcgca tgaggcgctc tacatggtat
     7141 tctttcacaa agccataccc gccgtgaatt tgtaccgcct ccaccgtatg gcgcatggct
     7201 acttccgaag cgtagagttt ggccatagcc ccggcagcgg tgtagtccat ttttttgtct
     7261 ttgagccatg ccgcgcgata aaccatcagc ttggcggcct caatttcggt ggccatatcg
     7321 gccaatttga aagcgatggc ctggtgctga ctgatgggct tgccgaatgc ctgacgctct
     7381 ttggagtacg ccgtggccag ttcaaacgcg ccgcctgcta tgcccaatgc ctgcgatgca
     7441 atgcctatgc gtccgccgga caaagttttc atagcaaatt tgaacccaaa tccatcttcg
     7501 ccgatgcgat tgcttttggg cactttcaca tcgttgtaca taatggagtg ggtatcggaa
     7561 gagcggatgc cgagt
//
LOCUS       DAORLF010000224         7570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5263_length_7570_cov_2.129341, whole genome shotgun sequence.
ACCESSION   DAORLF010000224 DAORLF010000000
VERSION     DAORLF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_7570_cov_2.129341"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>214
                     /locus_tag="PK198_16450"
     CDS             <1..>214
                     /locus_tag="PK198_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40385.1"
                     /translation="ALTEIADANDYDFELPPSERSHNGTAKIALISVERSLNAWALLM
                     DMMPGEEDAILSILAMLQRLQRMLQAE"
     assembly_gap    215..260
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            342..1238
                     /gene="era"
                     /locus_tag="PK198_16455"
     CDS             342..1238
                     /gene="era"
                     /locus_tag="PK198_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRF40386.1"
                     /translation="MHKSGFVNIIGRPNVGKSTLMNQLVGERMSIITNKPQTTRHRIF
                     GILSADDFQIVFSDTPGFIRQPSYRMQEIMNRFVGSSFEDADLMLFVTETGEEYGADD
                     PLLERMKSTGAPVFLVLNKSDLATEEQAQEVVDQWKNLVPFTQTFTISALKGIGTAEL
                     LEAILGALPEGPEYYPKDQLTDRPERFFVSEIIREKILELYHQEVPYSCEVGIEEFQE
                     TETKDGAPLARIRAEIYVARKTHKPIIIGKGGEAIKQLGSEARKAIEAFLDTKVFLEL
                     HVKVRENWRDDENALRRFGYQQ"
     gene            complement(1241..2233)
                     /gene="tsaD"
                     /locus_tag="PK198_16460"
     CDS             complement(1241..2233)
                     /gene="tsaD"
                     /locus_tag="PK198_16460"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRF40387.1"
                     /translation="MSDLILGIETSCDDTAVAVVDAEGSVRSSVVSSQLQTHAAFGGV
                     FPEFAAREHISNILPTVHEALRQAGIQPADLKAIGVTRGPGLIGSLLVGLHTATALGI
                     GWNKPVIGINHLRGHLRSADLEERRVVYPATILMVSGGHSLLAYMKNETDIRLLGATR
                     DDSVGEVFDKVGRMLGLGYPGGPEVDKLARQGQANIPFPRPLMQNNYEFSFSGLKSAV
                     SRYLAAHPDAVLADVAASFVQAVMEVLVKKSRLALKKNPSKSFLIVGGVAANSQLREE
                     AARLCQNLGVQLSLPPVRWSTDNGAMIALAAFDYLKLGIHKPPLPQLQLSINEF"
     gene            complement(2270..2902)
                     /locus_tag="PK198_16465"
     CDS             complement(2270..2902)
                     /locus_tag="PK198_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40388.1"
                     /translation="MLTLGISTSSNQFILALGEDNRLIFNSGDYESDKDIGALMLLAL
                     EKTQRSVQEIRRIVADIGPGGTSRVRTGVSFANGLAFSLSIGVCPVSLFELTGKLLWE
                     RHQLPVVSTINSLKGNAYIGFFNEGAMQPTRFGFIDQILPDMAAHLDAFVVAGPHSAA
                     IQALFPDKKIVLDNDLAGIAQIIMAHSSEWADRELKFPHIALPITEKHFL"
     gene            complement(2896..3381)
                     /gene="tsaE"
                     /locus_tag="PK198_16470"
     CDS             complement(2896..3381)
                     /gene="tsaE"
                     /locus_tag="PK198_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRF40389.1"
                     /translation="MSNRVLRFLSQQESDTMHIASVVGRRLRCGDAVLLEGDLGAGKT
                     HFVKGIAQGAGSEANVTSPTFSIANFYKTPGCDILHIDAYRIATEEDFLNLGLQDYFP
                     QCMVLAEWTQKFELLFDQYLFVALEYAEGNTRIITVSHKGGNFDAVFDALQQDCADLL
                     C"
     gene            complement(3507..3713)
                     /locus_tag="PK198_16475"
     CDS             complement(3507..3713)
                     /locus_tag="PK198_16475"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRF40390.1"
                     /translation="MKEGWVKIFSSIDLIQVKLVEDLLKQHGIESHILSKPDSVIPSI
                     GEAELYTIAEKAEEALEVVKTVQE"
     assembly_gap    3893..3905
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3907..>4266)
                     /locus_tag="PK198_16480"
     CDS             complement(3907..>4266)
                     /locus_tag="PK198_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40391.1"
                     /translation="PRFSTLRQVSHTFGGADITGNDNYAEKATGAALGWGGYLFGNEF
                     FALMFGMRIDYAITDFVSENGQADNYPAPYRFPGYDTYKPTNPLTGQIVLELNFGLGG
                     YAKTRCGQRTFVFGRRW"
     assembly_gap    4267..4287
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4288..4633)
                     /locus_tag="PK198_16485"
     CDS             complement(<4288..4633)
                     /locus_tag="PK198_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40392.1"
                     /translation="MKKQLSLLVFTALIFSSLHGQIWMDIGVKGSYGLGVLLNSNLMQ
                     DNSHEMRLGTGYAYGGKAGLNFGDRHGFTFDAILAKNSQTYDFKTSLIEVNNNELAWE
                     NLHLYVMYRMQAD"
     gene            complement(4774..5409)
                     /locus_tag="PK198_16490"
     CDS             complement(4774..5409)
                     /locus_tag="PK198_16490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF40393.1"
                     /translation="MSLRLGDVAPNFRAKTTEGEIDFYEYLGDGWGVLYSHPADFTPV
                     CTTELGRTAALKSEFEKRNVKVLALSVDPLDSHFEWIKDIEDTQHISMNFPIIADEHR
                     EVAELYDMIHPNATEKFTVRTVFVIGPDKKVKMTMTYPPSTGRNFHEILRVIDSLQLT
                     AYHSVATPVDWEHGQDVVIAPAVSDAEADVKFPKGYNKVRPYLRITPQPNL"
     gene            5600..>6305
                     /locus_tag="PK198_16495"
     CDS             5600..>6305
                     /locus_tag="PK198_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40394.1"
                     /translation="MKKINTTLKMTARFSALLALLVMCLPLLSAQDNGVIYLTNPSFE
                     DMPQPGRPPIGWMDCGFERESPPDVQPRQFGVTKAPQHGDSYLGMVVRANESWEAVTQ
                     RLSRPMEKGKCYEFSLYLCRAEFYLSPVSGDNPKDSANFVTPAKLRIWGGFSKCDKKY
                     LLYETKEIISHRWLEYACKLEPEDAYTYITLEAYYKTPVLFPYNGNVLIDNLSEIKPI
                     NCKQPVDDTPVADALPI"
     assembly_gap    6306..6330
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6331..6844
                     /locus_tag="PK198_16500"
     CDS             <6331..6844
                     /locus_tag="PK198_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF40395.1"
                     /translation="TPVTTPKPPVTTPKPPVTTPKPPVTTPKTGEPAATNKPEPAEGA
                     FANLRTEDLREGQTIRIDKLFFKTDSAVVLNTSYPMLEELYTFMSKNPEVSVEIGGHT
                     NSLPPDEYCDKLSTARAKFVYDYLIKKGIPTSRMQYKGYGKRKPIDTNNTADGRRNNQ
                     RVEVKILKLG"
     gene            complement(6849..>7570)
                     /locus_tag="PK198_16505"
     CDS             complement(6849..>7570)
                     /locus_tag="PK198_16505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF40396.1"
                     /translation="EYFKSWQALRSIQKAAELFPEHIPTLLKLSKTQLILKLYNESLK
                     SLDRVLKIDQNNADAHFLLGMNMKETGDTARAINSFQKAVNINSDLIDGWINLGQLHE
                     TKGHSVAGKYYETAVQVAGDNPLALHAKADFLSRRNDLNGAIDLYKKIQAMQPDYKEA
                     WFNAGLIYLDMDSIAEAYRQFDQAVVVSPLNVRAYFYRGYCAEQRGNKEQAEQDYRKA
                     LSFAPDYADALAGLERVKQPR"
BASE COUNT         1769 a         1987 c         1995 g         1714 t
ORIGIN      
        1 gccctcaccg aaatagccga tgccaacgac tacgattttg aactgccgcc ctccgaacgc
       61 agccacaacg gcacggccaa gatagccctc atctctgtcg aacgctccct caatgcctgg
      121 gccttgctca tggacatgat gcccggcgag gaagacgcca tcctcagcat attagccatg
      181 ttgcaacgac tccaacgcat gctccaggcc gagtnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn gaaggaaagg cctgatagcg cctgccggtt tctcagaaaa
      301 tgcaagtaca aaatcacatc gtctcaccgt cataccaaca catgcacaaa tcaggttttg
      361 tcaacatcat cgggcggccc aacgtgggca agtccaccct catgaatcaa ttggtgggcg
      421 agcgcatgtc catcattacc aacaaacccc aaaccacgcg gcaccgcatc ttcggcatcc
      481 tgagcgccga cgatttccag atcgtctttt ccgatacgcc cggcttcatc cggcagccct
      541 cgtaccgcat gcaggaaata atgaaccgct tcgtaggctc ttcgtttgaa gatgccgacc
      601 tcatgctctt cgtcaccgaa acgggcgagg aatacggcgc cgacgacccg cttctggagc
      661 gcatgaaatc taccggagcg ccggtttttc tggttctgaa caaaagcgac ctcgccaccg
      721 aagagcaggc gcaagaggtt gtagatcagt ggaaaaacct cgtgcctttt acgcagacat
      781 tcaccatttc ggcgctcaaa ggcatcggca ctgccgagtt gctggaggcc atcctcggag
      841 cgctgcccga aggcccggag tactacccca aagaccaact gaccgaccgc cccgaaaggt
      901 ttttcgtcag cgagatcatt cgcgaaaaga ttctggaact gtaccaccag gaagtgccgt
      961 actcctgcga ggtgggcatt gaggagtttc aggaaacgga aaccaaagac ggggcgccgc
     1021 tggcccgtat ccgtgctgag atatatgtgg cccgcaaaac gcacaagccc atcatcatcg
     1081 gcaagggcgg agaggccatc aagcagcttg gttcggaagc gcgcaaagcc attgaggcat
     1141 ttttagatac caaggttttt ttggaactgc atgtaaaagt gcgcgaaaac tggcgcgacg
     1201 acgagaatgc cctgcgccgg tttgggtatc agcaatgatt ttagaactcg ttgatactga
     1261 gttgcaattg cggtagcggc ggcttgtgaa tccccagttt cagatagtca aatgcagcca
     1321 gcgctatcat ggcgccgttg tcggtggacc agcggacggg cggcaggctg agttgtacgc
     1381 cgaggttttg gcagagccgg gccgcctctt cgcgcagttg tgaattggcc gccacgcccc
     1441 ccacgatgag gaaagatttg gaggggtttt tcttcaaggc caaacgcgat tttttgacga
     1501 gcacttccat cacagcctgc acaaatgaag ctgccacatc ggccaataca gcatccgggt
     1561 gggccgccag ataccgcgat actgccgatt ttaagcccga aaacgaaaac tcgtagttgt
     1621 tctgcatcag cggtcgcgga aacgggatgt tggcctgccc ttgccgggcc aatttgtcca
     1681 cctccgggcc gccaggatag cccagcccca acatgcggcc caccttgtcg aacacctcgc
     1741 ccaccgagtc gtcgcgggta gcgccgagca gccggatgtc ggtttcattt ttcatataag
     1801 ccagcaaaga gtgcccgccc gaaaccatga ggatggtggc cggatacacc acgcggcgtt
     1861 cttccagatc ggcgctgcgc aggtggccgc gcaggtggtt gatgccgatg accggcttgt
     1921 tccagccgat gcccaaggcc gtagccgtgt gcagccccac caacagcgaa ccgatcagtc
     1981 cggggccgcg cgtgacgcca atggccttca aatcagcggg ttgtatgccg gcttggcgca
     2041 gggcctcgtg tacggtggga agtatgttgg agatgtgttc gcgggcggca aattcgggga
     2101 acaccccgcc gaacgcagca tgagtttgca attgagagga aacgacggaa gagcgaacac
     2161 tgccctctgc atccactacc gccacagcgg tgtcgtcgca ggaagtttct atgccgagaa
     2221 tcaggtcaga catgttgcgg cgcaatggtt ttgacgggcc gaagcccttt tacagaaaat
     2281 gcttttcggt gatgggcagg gcgatgtgcg gaaactttag ttcgcggtcg gcccactcgc
     2341 tgctgtgcgc catgatgatc tgcgcaatgc cggccagatc gttgtcgagc actatttttt
     2401 tatcgggaaa gagggcctgt atggccgccg aatgtggccc ggccacgaca aaagcatcca
     2461 agtgcgctgc catgtcgggc aggatttggt caataaaacc aaaccgcgtg ggttgcatgg
     2521 cgccctcatt gaaaaagccg atgtaggcgt tgcctttcag cgagttgatg gtgctgacga
     2581 cgggcagttg atggcgttcc cagagcagtt ttcctgtcag ttcaaacaag gaaaccgggc
     2641 aaacgccgat gcttagactg aaggccaagc cattggcaaa cgacacgccg gtgcgcacgc
     2701 ggctggtacc gccggggccg atgtctgcca cgatgcgtcg gatttcctga accgagcgtt
     2761 gcgttttttc caaagccaac agcatcaaag cgccgatgtc tttgtcggat tcataatcgc
     2821 cggagttgaa aatcaggcgg ttatcttcgc ccagcgcgag tatgaactga ttggatgagg
     2881 tagaaattcc taaagtcagc atagtaagtc ggcgcagtcc tgttgtaaag catcgaacac
     2941 ggcgtcaaaa tttccgcctt tgtgcgacac ggtaatgatg cgggtattgc cttcggcgta
     3001 ctccaaagca acgaagaggt attgatcaaa aagcagctca aatttttggg tccattccgc
     3061 cagcaccatg cactgcggga agtagtcctg caagcccagg ttgagaaaat cttcttctgt
     3121 ggcaatccgg taggcgtcaa tatggaggat gtcgcagccg ggtgttttgt aaaaattggc
     3181 gatgctgaag gtagggctgg tcacattggc ctccgatccg gcgccctgag cgatgccttt
     3241 tacaaaatga gtttttccgg cgcccagatc gccctccaac agaacggcat cgccgcaacg
     3301 gagccgccgc ccgaccaccg aagcgatgtg catggtgtcg ctttcctgtt gagacaaaaa
     3361 ccgtagtacc ctgttggaca ttttgatcaa gttgacccta tccgcgcgat tgctctgtta
     3421 tctaaaaaac cacgtttgcc ggattcgggc cgcaaaggta ggcatacaag gcgggaatca
     3481 caaacaccca ctcactcctc actcactcac tcctgaaccg tcttcaccac ttccagcgcc
     3541 tcctctgctt tttcggcgat ggtatagagt tctgcctcgc cgatagaagg gatgacggag
     3601 tccggtttgg aaaggatatg gctttcaatg ccatgttgtt tgagcaggtc ttctaccaat
     3661 ttgacctgaa tgaggtcaat agaagaaaat attttcaccc atccttcttt catggttatt
     3721 gtggttttga ttcggtagta aatgtaacgg tttttggcca gtattgcaag gtcatatacc
     3781 atgccgccat acggggatca ctccaactcg ctgatgttca ggtggtatgg agatgtattc
     3841 tttttgtaaa atctcctttt cccattgccc ggcggggcgt aaggggacga ttnnnnnnnn
     3901 nnnnntttac caccttcttc cgaatacaaa agtgcgttgg ccgcagcggg ttttggcata
     3961 gccgcccagc ccgaagttca gttccagcac gatttggccg gtgagcggat tggtgggctt
     4021 gtaggtatcg tagccgggaa aacgatatgg agcgggataa ttgtctgcct ggccgttttc
     4081 gctcacaaaa tccgtgatgg catagtctat cctcatgccg aacatgagcg caaaaaattc
     4141 atttccaaac aaatagccgc cccagcccaa tgcggcgccg gtagcttttt cggcgtagtt
     4201 gtcgttgccg gtaatatctg ctccgccaaa agtatgcgat acctgccgga gggtggaaaa
     4261 gcgcggnnnn nnnnnnnnnn nnnnnnncgt cggcttgcat gcggtacatg acgtagaggt
     4321 gcaggttttc ccaggccaat tcgttgttgt tgacttctat gagggaggtt ttgaaatcgt
     4381 aggtctgtga gtttttagcc agaatagcat caaacgtgaa gccgtggcga tcgccgaaat
     4441 tcaggccggc tttgcctccg taagcgtacc cggtgccgag gcgcatttcg tgggagttgt
     4501 cctgcatgag gttggaattg aggagtacgc ccagcccgta gctgcctttg acgcctatat
     4561 ccatccagat ttgcccgtgc agggagctga aaatcaatgc agtaaaaacg aggagggaaa
     4621 gttgcttttt catgttttgt ttgttttggt gaatgttgag ttagatgctc ttgccggcaa
     4681 aagtgctgcc tgagcagcga gaaaacaaac atcccctgcc gaccgactcc gagtacgaca
     4741 ggggatgcaa agagatagaa taaatggacc gaatcagagg ttcggttgcg gcgtgatgcg
     4801 cagataggga cgaaccttat tgtatccttt cgggaatttc acatcggctt ctgcatcgga
     4861 aactgcgggc gcgatgacca catcctgccc gtgctcccaa tcaactggcg tggcaacact
     4921 atgataagca gtcagttgca gagagtcaat gacgcgcaaa atttcgtgaa aattcctacc
     4981 ggtgctgggc gggtacgtca tggtcatttt gacctttttg tctggcccga tgacaaaaac
     5041 cgttctcacc gtaaactttt ctgtggcgtt ggggtgtatc atgtcgtaca actcggccac
     5101 ttcgcggtgc tcgtcggcta tgatgggaaa gttcatggaa atatgctgtg tgtcctcaat
     5161 atccttgatc cactcaaaat gagaatccaa gggatcaacg ctgagggcaa gcactttcac
     5221 attgcgtttt tcaaactcgg atttaagggc agccgttcgg cccaactcgg tggtacaaac
     5281 cggcgtaaaa tcggccgggt gagagtacaa tacaccccag ccatcgccga ggtactcgta
     5341 aaaatcaatt tcgccttctg ttgttttggc gcggaaattg ggcgccacat cgcctaatcg
     5401 caagctcatg atcattggtt ttaagaagtg aagccattat tggccgtgca aaagtaatca
     5461 ttttttaatt tcctattaaa aaggtaggat aaaattgttt ttgcgccaaa aacattgttt
     5521 ggctgtttgg ctttaagtct gtgtattaaa aaacatactt tggataaacc aattacaggt
     5581 agctttgcgt ttgtaaaccg tgaagaaaat aaacactact ttgaaaatga ctgctcgttt
     5641 ttcggcgctg ttggcgctat tggtaatgtg cctgcctttg ctctccgccc aggacaacgg
     5701 agtgatttat ctcaccaatc cctcttttga ggacatgccg cagccgggcc gcccgcccat
     5761 tggctggatg gactgcggtt ttgaacgcga gtctccccct gatgtgcagc cacgtcagtt
     5821 tggtgtaacc aaagctccac agcacggcga ttcttacctc ggcatggtgg tgcgggccaa
     5881 tgaatcctgg gaagcggtga cgcaacgcct gagccgcccc atggaaaaag ggaagtgcta
     5941 cgaatttagc ctctacctgt gccgggccga gttctacctc agccctgtat cgggagacaa
     6001 ccctaaagat tctgccaatt ttgtaacgcc ggccaagctg cgcatctggg gcgggttttc
     6061 caaatgcgac aaaaaatacc tgctctacga aaccaaagaa atcatcagcc accgctggtt
     6121 ggagtacgcc tgcaagctgg aacccgaaga cgcctacact tacatcacgc tggaggctta
     6181 ttacaaaacg ccggtattgt ttccttacaa tggcaatgtg ctcatagaca acctttcgga
     6241 aatcaagccg atcaattgta agcaaccggt ggacgatacg cccgtagccg atgcactgcc
     6301 cataannnnn nnnnnnnnnn nnnnnnnnnn aactccggtg acgacgccca aaccgcctgt
     6361 aacgacgcct aaaccgccag tgacgacgcc caagcctccc gtaacgacgc ccaaaacagg
     6421 cgaaccggcc gccaccaaca agcccgaacc ggcagaaggg gctttcgcca acttgcgtac
     6481 tgaagacctc cgcgaaggcc agaccatccg cattgacaag ctgtttttta aaaccgacag
     6541 cgcggtggtg ctcaatactt catacccgat gctggaagag ctttatactt tcatgtcgaa
     6601 aaacccggag gtgagcgtgg aaataggcgg ccataccaac agcctgccgc cagatgagta
     6661 ttgcgacaag ctctcgacgg cgcgcgccaa gtttgtgtat gattacctga tcaaaaaagg
     6721 catccctaca tcgcgcatgc agtacaaagg ctacgggaaa cgcaagccga tagataccaa
     6781 caataccgca gacggcaggc gcaataatca acgggtggag gtgaagattt tgaagctggg
     6841 gtaagccctc acctcggctg tttcaccctt tccaatccag ccagcgcatc ggcatagtcc
     6901 ggcgcaaaac tcagcgcctt gcggtagtcc tgctcggcct gctctttgtt gcccctttgc
     6961 tcggcgcaat agcccctgta aaagtaggcc cgcacattca agggcgacac gacgaccgct
     7021 tggtcaaact gccggtacgc ctccgcaatg gagtccatat caaggtaaat gaggcctgca
     7081 ttgaaccacg cttctttgta atcgggctgc atggcctgaa tttttttata caaatcaata
     7141 gcgccgttca ggtcgttgcg tcggctcaga aagtcggcct tggcgtgcag ggccagcgga
     7201 ttgtcgccgg ctacctgtac ggcggtttca taatatttgc cggccaccga atggcctttc
     7261 gtttcgtgca gttgccccag attgatccag ccgtcaatga ggtcggaatt gatgttcacc
     7321 gccttctgaa aactattgat ggcacgcgcc gtgtcgccgg tttccttcat gttcatgccc
     7381 agcaggaaat gtgcgtctgc attattttgg tcaattttca atacgcgatc caacgacttc
     7441 aacgattcat tgtatagttt caaaatcagt tgtgttttac tgagtttgag cagggtaggg
     7501 atgtgttcgg gaaacaactc cgctgctttt tggatactgc gcagggcctg ccatgatttg
     7561 aaatactcca
//
LOCUS       DAORLF010000225         7544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5291_length_7544_cov_2.063426, whole genome shotgun sequence.
ACCESSION   DAORLF010000225 DAORLF010000000
VERSION     DAORLF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_7544_cov_2.063426"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK198_16510"
     CDS             complement(<1..>214)
                     /locus_tag="PK198_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF40397.1"
                     /translation="LAVAIAVALTLDLSINVGFNPTRTIIADVTPEGHPRTKGYTWMQ
                     TISGTFGMSAYLISAIWDNYTLIYAGA"
     assembly_gap    215..417
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(624..>795)
                     /locus_tag="PK198_16515"
     CDS             complement(624..>795)
                     /locus_tag="PK198_16515"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRF40398.1"
                     /translation="ASRIAEGSVDMVIFFRDPLGKHPHEVDVSMLMRLCDVYNIPLAT
                     NPAAAQRFVQTL"
     assembly_gap    796..805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<806..992)
                     /locus_tag="PK198_16520"
     CDS             complement(<806..992)
                     /locus_tag="PK198_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRF40399.1"
                     /translation="MTIAFIAHDGKKAEMVGFVKDYVELFKSKNITLIATGTTGSHLE
                     RAGLEVNKMASGPMGGDA"
     gene            1098..1997
                     /locus_tag="PK198_16525"
     CDS             1098..1997
                     /locus_tag="PK198_16525"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF40400.1"
                     /translation="MKKLILPVFVMFMTALSIHTLEAQIRTPQPSPAAEVKQTVGLTE
                     VSIVYSRPSAKGRVVFGDLVPMDQVWRTGANSATKITFSENVKLGGQEVKKGSYALLT
                     VPGKDMWKVMLHNYESTNWGSYTDKAAAVAFSVKSDKTAEFTETFTIAVNDVTSNSAN
                     IDIRWENTKASLPLMVEFDAAVMENIKRVMAGPSAGDLAAAANYYHDNGKDLNQALEW
                     IQKANASTPRYWTLRREAMILADLGRYEEAVKTAEKSLALASEGGDDAYVKMNKKSIE
                     EWTPKIKPAQQPATQPKGKKGKS"
     gene            2289..2819
                     /locus_tag="PK198_16530"
     CDS             2289..2819
                     /locus_tag="PK198_16530"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRF40401.1"
                     /translation="MSKKVILYIATSLDGFIARENGSVDWLPQSGEDFGYEAFLSQID
                     TVLMGHKTYRQILSFGVEYPYTGKRNYVFTRDKTLERDEHAEFVHSNIVPFVKALKVS
                     GFKNIWLVGGGELFSELLAAGVVDELILFVFPLLLGEGIPLFQGPFPEQALELLHAHA
                     HKGGVLELRYGIGSHM"
     gene            3007..3213
                     /locus_tag="PK198_16535"
     CDS             3007..3213
                     /locus_tag="PK198_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40402.1"
                     /translation="MHYLRLTSVITFLLIMLVLILFIYPTATAFMLASALTPLLIGVL
                     AVRVLRAPVQAPERKTDSDWYDVH"
     gene            complement(3220..4155)
                     /locus_tag="PK198_16540"
     CDS             complement(3220..4155)
                     /locus_tag="PK198_16540"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRF40403.1"
                     /translation="MKNKHLVIIFAMLLVAVYFSRKQFGKRERSFESVLIEADTSAVS
                     SVVIHAPGETEFSLVKESGKWILTDGRRNISAEAEPVGQLLAALMKVETQQVVAKDKD
                     LWTVYGVDDAHATRVKLYERGTLTQDFLLGNEDLDAASQSTITFLRISDEKAVFVVDG
                     FQMSHIGKTFNSYRNRLLLRMKREMEMNAFLWESPDTTLAFERGANGWTLGGTVLDSM
                     AVEEYLNAFRNISADDFADDFDELEADDKLHKRLVIKGKNIPGVFEITCFSDAARKPP
                     FVLVSSQNPKTFFSSDSTGLYGKIFPAVEGFYKMQ"
     assembly_gap    4423..4432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4433..5793)
                     /locus_tag="PK198_16545"
     CDS             complement(<4433..5793)
                     /locus_tag="PK198_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF40404.1"
                     /translation="MRFTIFSFLLTLSSLALAQSPEHTAPQYEVESHLRFLASDELQG
                     RRTGEPGNFIAARYIAENFRAFGAQPLPGADGYFQHFPLETVTPPSASALKIGKTAYE
                     HRKDYLMMAGDALNAKTTAVFAGHGWVDASAGHDDYKGLDVKGKVVFVISGLPDDSSP
                     MAAFNSIRDKRRLAQERGAVAIIELYRLGFPWQFALQYFGKSSTRVGDDSPDKATIPY
                     GWLKEKGNDALAEIVKGAPQKITLSSSGASVTRKMVPNVIAVIPGSDPVLKDEYVLLS
                     AHFDHVGTGKNGGSPFTAADSIFNGARDNAFGTSALLLAARTLAQQKTKRSIILLACN
                     GEEMGLLGSLYYADHPLIPLRQTIFNLNADGAGYNEVTKISAIGHGRTGTDEHIESAA
                     AAFGLGVIPNPVPEQGLFDRSDNVSFAAKGVPAVCISPGVTGFDETLMKYYERMSDET
                     RRD"
     gene            complement(5827..6459)
                     /locus_tag="PK198_16550"
     CDS             complement(5827..6459)
                     /locus_tag="PK198_16550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRF40405.1"
                     /translation="MNELIDISVPLHPGLPLWPETPALRITQDMSLAAGDVANVSRLD
                     LDAHSGTHIDAPLHFIPGADTTDDIPLTQLIGPCLVADFTGLQRITAADLEAAHIPDD
                     TRRLLLKTDNSALWQDFRRPFRPDFCALSLDAAQWIAERDMFLVGIDYHSIQMYDDPF
                     DTHIVLLQHKVVILESLHLHHVAPGPYELLCLPLRVWGLEAVPVRAVLRR"
     gene            complement(6452..>6983)
                     /locus_tag="PK198_16555"
     CDS             complement(6452..>6983)
                     /locus_tag="PK198_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40406.1"
                     /translation="IDILINNAGKAKGFDFIHEGSLEHWDEMIDTNVKGLLYVTRAVS
                     PRMVARRKGFIINIGSTAGKEIYPKGNVYCASKFAVDALTKATRYDLHNHNIRVGQVS
                     PGHVEETEFALVRFDGDAERAKIYNDFKPLTSPDVAEAIYFMATQPPHVNIQDILMLG
                     TQQAGNMLIDRSGRHE"
     assembly_gap    6984..7148
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7149..>7544)
                     /locus_tag="PK198_16560"
     CDS             complement(<7149..>7544)
                     /locus_tag="PK198_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40407.1"
                     /translation="RTALQLQPAHPFVWYDLALLYHRLGDSEEAYTAYQNAVLNNPEL
                     KTPQNDVAFEYLRSAHTAKAAEIIEQEQAALAEMRENLHRLEALLKEREAELQRLTER
                     PVNNQTVFITGATSGIGRATAVKFAQNGF"
BASE COUNT         1555 a         1816 c         2126 g         1659 t
ORIGIN      
        1 cgcgcctgca tagatgagcg tgtaattatc ccagatggcg cttattaaat aggccgacat
       61 gccgaatgtg cccgaaatgg tttgcatcca ggtgtagccc ttggtgcgcg gatggccttc
      121 gggcgtcaca tcggcgatga tggtgcgggt aggattgaaa cccacattga tggacaaatc
      181 cagcgtaagc gccacagcaa tcgccactgc cagannnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
      421 tcgtcaattt tcaggttgta cttggtggtc agaatccagc tcagcgcggc aatttgtacc
      481 gaaagcgcaa aacccatcgc agaggagggc aagctgagga tgccgtaaaa cagcggcgaa
      541 agacgttttt gaatatcgag catgacagtt gttttaattt cggattcaaa attccggatt
      601 caagatttcg gattttgctg tttttacaaa gtttgtacaa aacgttgggc ggctgcggga
      661 ttggttgcca gcggaatatt atacacatcg cacaggcgca tgagcatgct cacgtccacc
      721 tcgtgcgggt gcttgcccag cgggtcccga aaaaaaataa ccatgtccac cgagccttct
      781 gcaatgcgcg aagctnnnnn nnnnngcgca tcgccgccca taggtccgct ggccattttg
      841 tttacctcta agccggcgcg ttccagatga gaaccggtgg tgccggtggc gatgagcgtg
      901 atgtttttac ttttaaaaag ctccacataa tcctttacga agcccaccat ttcagctttt
      961 ttgccgtcgt gagcgataaa tgcgatggtc atattcaacc ccttttgatt ttgactgttt
     1021 gggaaagtgt tgtgtgtagt tacacctatt gtgcataact tcgcgccaaa tttagcataa
     1081 tcaaaaccaa gtttaatatg aaaaaactca ttttacctgt tttcgtcatg ttcatgaccg
     1141 ccctgagcat ccacacgctc gaagctcaga ttcgcacgcc gcagcccagc cctgccgccg
     1201 aagtgaaaca aacagtgggc ctcaccgagg tctccattgt gtattcccgc cccagcgcca
     1261 aaggccgcgt ggtgtttggc gatctggtgc cgatggatca agtttggcgt acgggcgcca
     1321 actcggccac caaaatcacg ttcagcgaga acgtcaaact cggtggtcag gaagtgaaaa
     1381 aaggctctta tgccctcctc actgtgcccg gcaaagacat gtggaaggtg atgttgcaca
     1441 attacgaaag caccaactgg ggcagctata cagacaaagc agccgcagtg gcattttccg
     1501 taaaatcaga caaaaccgct gaattcacgg aaactttcac cattgcagtc aatgacgtga
     1561 ccagcaactc tgccaacatt gacatccgct gggagaacac caaagccagc ctcccgctca
     1621 tggtagaatt cgatgccgcc gtgatggaaa acatcaagcg cgtcatggcc ggcccctctg
     1681 ccggcgatct ggctgctgcc gccaactact atcacgacaa cggcaaagac ctcaaccagg
     1741 ctttggaatg gattcagaaa gccaacgcaa gcacgccgcg ctactggacg ctgcgtcgcg
     1801 aggccatgat tctggccgat ctgggccgct acgaagaggc tgtcaaaact gctgaaaaat
     1861 cgctggcttt ggcttccgaa ggcggcgacg acgcttatgt gaaaatgaac aaaaaatcta
     1921 ttgaagagtg gacgccgaag atcaagcctg ctcagcagcc tgccacgcag cctaaaggca
     1981 aaaaagggaa aagctgattt ttagcttttg ccctggaaag cctcgaaagt cataagattt
     2041 tcggggcttt tttttggggc ttgccaaagc aacgtttgcg aagcctcaca ggattcgtaa
     2101 acgtagtacc gagaatacct tgaatttggc gtgccggccc actcatacca tcaaaagggg
     2161 ccgtcgggcg cctcggtcgt cctcctatcg tcgttcctcc cttcggcccc ctcacgcgca
     2221 ggcttacacg gcaaaatcca gagttgtttt tcggcaggca gcgttgtatt ctgttttata
     2281 tttgcagcat gagcaaaaaa gtaattctct acatcgccac cagcttagac ggcttcattg
     2341 cccgcgaaaa cggctcggtg gactggctgc cccagagcgg cgaagatttc ggatacgaag
     2401 cctttctctc tcagatagat acggtgttga tgggacacaa aacctaccgc caaatcctga
     2461 gctttggagt ggaatacccc tacacaggca aacgcaacta cgtcttcacc cgcgacaaaa
     2521 ccttagaacg ggacgagcac gcagagtttg tccacagcaa catcgttcct tttgtcaaag
     2581 cgctgaaagt ctccgggttt aagaacatct ggcttgtcgg cggcggcgaa ctgttctccg
     2641 aattgctggc tgccggcgtg gtggacgaac tgattctctt cgtgtttccg ctgctgctgg
     2701 gcgagggcat tccactgttt caagggccct tccctgaaca agcacttgaa ctgctgcacg
     2761 cgcacgcaca caagggaggc gttttggaac tgaggtacgg aatcggcagt cacatgtagc
     2821 gcggtgggaa cccggaacca gtaggcggta ggcagtgagc ggttccaacg caaaaccttg
     2881 aacgcaaaac cttgaacccg aaacccacct tcccacgcct gtggcgtggt tcggcggatg
     2941 caacccgaaa ccttgaaccc gccaacataa cgccgccacg attgttagac cagaaaatca
     3001 acagctatgc actatctgag gcttacttcc gtcatcactt ttttgttgat catgttggtc
     3061 ttgatcctgt tcatctaccc cactgcaacg gcatttatgc tggcctcggc gctgacgccg
     3121 ctgctcatcg gagtgttggc cgtcagggtg ttgcgcgcac cggtacaggc tcctgagcgc
     3181 aagaccgaca gcgattggta cgatgtgcat tgacctgatc tactgcattt tataaaatcc
     3241 ctccaccgcc ggaaatatct tcccgtataa gcccgtgctg tcggaagaaa aaaacgtctt
     3301 tgggttttgt gatgaaacca acacaaaggg cggcttgcgg gcagcatcgg aaaaacaggt
     3361 gatttcaaaa acacccggta tattcttgcc tttgattacc aacctcttgt gcagtttgtc
     3421 gtctgcttcc aactcgtcga aatcatcggc aaaatcatca gcagaaatat tgcgaaaggc
     3481 attcagatac tcctctaccg ccatcgaatc caacacagtg cctcccagcg tccagccgtt
     3541 ggcgccgcgc tcaaaggcca gggttgtatc ggggctttcc caaaggaagg cgttcatttc
     3601 catttcccgt ttcatgcgca agagcaggcg gttgcggtag ctgttgaaag ttttgccgat
     3661 gtgcgacatc tgaaatccgt ccactacaaa aacggccttt tcatcgctga ttctcagaaa
     3721 tgtaatggtg gactgagacg cggcgtccaa atcctcgttg cccagaagga aatcttgtgt
     3781 cagcgttccc ctttcgtata gttttacgcg ggtggcatga gcatcgtcca cgccatacac
     3841 cgtccacagg tctttgtctt tggctacaac ttgttgcgtt tccactttca tcagggcggc
     3901 cagcaattgt cctaccggtt ccgcctcggc cgagatattg cgccggccgt cggtcagaat
     3961 ccatttgccg gattctttga cgaggctgaa ttcggtttcg cccggtgcat ggatgactac
     4021 cgaactgacc gcggaagtgt cggcttcgat gagtaccgac tcgaagctgc gctcccgttt
     4081 accaaactgt ttgcgcgaga aatagaccgc taccaacagc atggcaaaaa tgatgactaa
     4141 gtgtttgttt ttcataggtt tactgttcaa ggtttcgggt ttaaggtttc gggttcagca
     4201 tttcgggggt agtgaagggg tgactttgca agtttcagcc tcgcagtcac cccttcacta
     4261 agccggagaa cccggaaccc ggaactattt ctgatacagt ttcatgccgg cgctttcata
     4321 tttgtcgcct tttttccaga tgggtttggc ggcatcgtcg gcgatgcggc gtgccagcaa
     4381 agcaaagctc tgacagtagc gcaagaggta cggcatgtcc acnnnnnnnn nnttgtcgcg
     4441 ccgtgtctca tcgctcatgc gttcgtagta tttcatcagc gtttcgtcaa agccggtgac
     4501 gccggggctg atgcagacgg cgggcacgcc tttggcagca aaagaaacgt tgtcggagcg
     4561 gtcgaaaaga ccttgttcgg gcacaggatt ggggatgaca cccagaccaa atgcagcagc
     4621 cgcactttcg atgtgctcgt ctgttccggt gcggccatga ccgatggcag aaattttcgt
     4681 cacctcattg tagccggcgc catcggcatt gaggttgaag atggtttgac gcagcggaat
     4741 gagcggatgg tcggcgtagt aaaggctgcc gagcaggccc atttcttcgc cgttgcaggc
     4801 cagcaggatg atggaacgct ttgttttttg ctgtgccagg gtacgggcgg cgaggagcag
     4861 tgccgaagtg ccgaaagcat tgtcgcgggc gccgttgaaa atgctgtctg ctgcggtgaa
     4921 gggagagcct ccgtttttgc cggtgcctac atgatcgaag tgggcgctga gcaggacata
     4981 ctcgtctttg agtacggggt cggagccggg aatgacggcg atcacattgg gtaccatttt
     5041 gcgagtgacc gaagcgccgg agctgctgag cgtgattttt tgaggggcgc ctttgacgat
     5101 ttccgccaaa gcgtcgttgc ctttttcttt gagccagccg taggggatgg tggctttatc
     5161 gggcgagtcg tcgccgacgc gggtggagga cttgccgaag tactggagcg caaactgcca
     5221 ggggaagccg aggcgataca gttcgatgat ggctactgcg ccgcgctcct gagccaggcg
     5281 gcgcttgtcg cggatgctgt tgaaagcggc catcgggctg gaatcatcgg gcaggccgga
     5341 gatgacaaaa acgactttgc ctttgacgtc gaggcccttg tagtcgtcgt ggcctgcgga
     5401 ggcgtccacc cagccatgac cggcaaaaac ggcagtggtt ttggcattga gggcatcgcc
     5461 ggccatcatg agatagtctt tgcggtgctc gtaggcggtt ttgccgattt tgagggcgga
     5521 ggccgagggc ggcgtcacgg tttcgagagg gaagtgctgg aagtagccgt cggcgccggg
     5581 caggggttgc gcgccgaagg ctcggaagtt ttcggcgatg tagcgggcgg caatgaagtt
     5641 gccgggttcg ccggtgcggc ggccctgaag ctcatcggag gctaagaagc ggagatggga
     5701 ttccacttcg tattgcggcg ccgtgtgttc gggtgattgg gcgagcgcta agctgctcaa
     5761 tgtcaggaga aaagaaaaga tagtgaatcg catgttgaat cgttttgatg gtttcatatt
     5821 caaagttcat cttcttaaaa cagcgcgcac gggtacggct tcgaggcccc aaacgcggag
     5881 cggcaggcag agtagctcgt aagggccggg agcgacgtgg tgcaggtgga ggctttcgag
     5941 gatgacgact ttgtgttgca gcagtacgat gtgcgtgtcg aaagggtcgt cgtacatttg
     6001 gatggaatgg tagtcaatgc cgacgaggaa catgtccctt tctgcgatcc actgtgctgc
     6061 atcgaggctg agggcgcaga aatcggggcg gaaaggccgg cggaaatctt gccacagcgc
     6121 ggaattgtcg gttttgagga gaaggcggcg cgtgtcgtcg ggaatgtgtg cggcttcgag
     6181 gtcggcggcg gtgatgcgtt gcaggccggt gaaatcggcc actaagcagg ggccgatgag
     6241 ttgtgtgagc ggaatgtcgt cggtggtatc ggcgccggga atgaagtgca gcggcgcgtc
     6301 aatatgggtg ccggagtgcg cgtcgaggtc gaggcgactg acgttggcca catcgccggc
     6361 ggcgaggctc atgtcctgtg tgatgcgcag ggcgggcgtt tcgggccaga ggggcaggcc
     6421 cggatggagc ggcacggaga tgtcaatcag ttcattcatg ccgtccgctg cgatctatga
     6481 gcatgttgcc ggcctgctgc gtgcccagca tgaggatatc ctggatgttg acatgcgggg
     6541 gctgggtggc catgaaatag atggcttcgg cgacgtcggg ggaggtgagc ggtttgaaat
     6601 cgttgtagat tttggcgcgc tcggcatcgc cgtcgaagcg aacgagggcg aattccgtct
     6661 cctccacatg accaggactg acctgcccca cgcggatgtt gtggttgtgg aggtcgtagc
     6721 gggtagcttt ggtgagggcg tccacggcga atttggaggc gcaatagacg ttgcctttcg
     6781 ggtaaatctc tttgccggca gtggaaccta tattgatgat gaagcctttg cgacgggcca
     6841 ccatgcgcgg agatacggcg cgggtgacgt agagcaggcc cttgacgttg gtgtctatca
     6901 tttcgtccca gtgttcgagg ctgccttcgt ggatgaagtc gaagcctttg gctttgccgg
     6961 cattgttgat gaggatgtcg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnga aagccgtttt gggcaaattt gacggcggta gccctgccga tgcccgatgt
     7201 ggccccggtg atgaataccg tttggttgtt gacggggcgc tcggtgaggc gttgtagttc
     7261 tgcctcgcgc tctttgagca gtgcttcgag gcgatgtagg ttctctcgca tttccgccag
     7321 agcggcctgc tcctgctcga tgatttcagc ggctttggcg gtgtgcgcgg agcgcaggta
     7381 ctcaaaagcc acgtcgtttt gcggggtttt cagttcggga ttgttgagga cggcattttg
     7441 ataggccgtg taagcttctt ctgaatcacc gagacgatgg tacagcaggg ccagatcgta
     7501 ccatacgaag gggtgcgcgg gctgaagctg cagcgcagtg cgaa
//
LOCUS       DAORLF010000226         7538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5297_length_7538_cov_1.975277, whole genome shotgun sequence.
ACCESSION   DAORLF010000226 DAORLF010000000
VERSION     DAORLF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_7538_cov_1.975277"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK198_16565"
     CDS             complement(<1..335)
                     /locus_tag="PK198_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF40408.1"
                     /translation="MDNNATTPTDPRVVDAMLPFFYQKPGNAASRNHPFGWEAEEAVD
                     YAREQIADLIHVDSKEIIFTSGATEADNLALKGVFEMYHSKGNHIITTKTEHKAVLDS
                     CKKIEKMGG"
     gene            612..1205
                     /locus_tag="PK198_16570"
     CDS             612..1205
                     /locus_tag="PK198_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40409.1"
                     /translation="MMLYAESDIDFHFSTADWAVCRYDRHRYFKGWSGRGLKGVDFIA
                     VWRQDKLIFMEVKNYNPRPNGKGGQTADAVRADPAILSDAFGEKIADTLTAIDAVRQY
                     RQRRWWRRLAAWWFRRRKVGSSETAFWVRVCDLALSPQNCMAVLWLEGEELDASFRKT
                     IEKRLAYELSALTAQVVVCNADSNPFREDGLTAKIAE"
     gene            complement(1212..2225)
                     /gene="nadA"
                     /locus_tag="PK198_16575"
     CDS             complement(1212..2225)
                     /gene="nadA"
                     /locus_tag="PK198_16575"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRF40410.1"
                     /translation="MSTLTLAQNNLERKGFLDLDVDPTLDLVQEINRLKREKNAVILA
                     HYYQDADIQDIADYIGDSLGLSQKAAATDADIIVFAGVHFMAETAKMLSPHKKVLLPD
                     LKAGCSLADSCPAPVFREFKKKYPGHVVVSYINCTAELKTLTDICCTSTNALHIINSI
                     PEDQPIIFAPDINLGKFLVQKTGRDMVLWNGACMVHEIFSHEKIVKLRQRHPDAKFIA
                     HPECEPHILEMADYVGSTTGLLKYTISDAATEYIVATESGILHQMQKASPHKTFIPAP
                     PDNACACNDCPHMKLNTLEKLYLCMEYEQPEIILPQWIIDDGRACIDRMLDISAKAGL
                     VGG"
     assembly_gap    2385..2417
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2877..3119)
                     /gene="rpmA"
                     /locus_tag="PK198_16580"
                     /pseudo
     CDS             complement(2877..3119)
                     /gene="rpmA"
                     /locus_tag="PK198_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
     gene            complement(3158..3472)
                     /gene="rplU"
                     /locus_tag="PK198_16585"
     CDS             complement(3158..3472)
                     /gene="rplU"
                     /locus_tag="PK198_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRF40411.1"
                     /translation="MFAIVTIAGQQFKVEEGQEIFVHQLEAAEGDSLSFDQVLLVERD
                     GQTSVGTPVLNAAKVNATVVSGHVKGDKVIIFKKKRRKGYRLKKGHRQHFTKLKIDSI
                     VA"
     gene            complement(3553..4860)
                     /locus_tag="PK198_16590"
     CDS             complement(3553..4860)
                     /locus_tag="PK198_16590"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF40412.1"
                     /translation="MTDKPEDILLREVQYLLGKPPEWILRRGTGITLTGALLAIVVAW
                     FIRYPDVVTAPVSIVTDRPAIHLVSISGGTIAGVLVKEKDQVAEGDVLICVDNPAKWE
                     HVLALEKLMDALLSDSVDMDEPTTEDLLSDWELGSMQSSYSEWLVALEKFMLEKGRLY
                     SIQGVAALRQQIKDLQLLNTSLKQQEETTKKEEELAATRLSKAEEKYKQGAIKEQEFQ
                     IDREIYLGKKSKVEALQTEQLNNQVKIGNLRKELADMGFSQLGGMRSSDIRLRELTRN
                     LKSEILFWKQNYLVTAPVDGIVSLASNWTLGQYLRPGQEVATLLAPEGQGNIRAQGLL
                     PTTGAGKVAPDMSVRIRLAGFPYKEYGVLTGYVGDIALAPVSTDNAPSYRINIELPSG
                     LKTNYGRTLPLLRESQGTAYIITERRNLLQRLFQPLYSALKNN"
     gene            complement(4877..>5067)
                     /locus_tag="PK198_16595"
     CDS             complement(4877..>5067)
                     /locus_tag="PK198_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40413.1"
                     /translation="PAYYNRGTRFYFNLRYKPTRALTVEARIAQTYWANQKTFGSGLE
                     EIAGPARTQLTLQTKVQF"
     assembly_gap    5068..5095
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5096..>5664)
                     /locus_tag="PK198_16600"
     CDS             complement(<5096..>5664)
                     /locus_tag="PK198_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF40414.1"
                     /translation="VAVLHRYYARDFQTLTGDAFGESGGVRNEHGLYFGLEMRPMRGW
                     KINAYFDTWQHPWLKFNADGPSRGHEYRLRITHIIKRKLTAYAEFRRENKELNFRNNA
                     TAIDYLAGTQLTQARFFVGHQVNKMLELRTRLDLGRFDDGSGTMSNGLALYQDVLFRP
                     LGFPLSFTTRFALFDTDGYAVRFYAFENNL"
     assembly_gap    5665..5703
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5704..>6054)
                     /locus_tag="PK198_16605"
     CDS             complement(<5704..>6054)
                     /locus_tag="PK198_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF40415.1"
                     /translation="EADTTDVEGFDPEIREITSINLSGLHRTTSEIADKNALTQFTTG
                     ASLKWKPGSNHLAFNAVYTSLDKFIAPRTQPYARYYFSGNSLVNASMDYGLILRNFHF
                     FGENAISDNGAIAAL"
     assembly_gap    6055..6101
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6102..6974)
                     /locus_tag="PK198_16610"
     CDS             complement(<6102..6974)
                     /locus_tag="PK198_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF40416.1"
                     /translation="MRRLLAILLLLSCALALSAQKDTLPPAPAEGNTDLLEDLLQNTD
                     ADAVGDFNTLFESLGDYLRKPLNINKADEADLRDLLLLNDVQIAGLLRHRAIAGDLIS
                     VYELQAVPGFTPELIRALMPYITVGGDLDDYQVSIKEMLRDGSNELYLRWARVLEQQK
                     GFTTAADTADSRYLGDPNQYYLRFRHSYGTKLSIGLTAEKDRGEQFFAGANRYGFDFY
                     SAHIALRNYNRTIKAVVLGDYAVSFGQGLIMLTGFNYGKTIQSVGLKRGGRALRPYTS
                     VNEAVFQRGAAAQLS"
     gene            complement(7025..>7538)
                     /locus_tag="PK198_16615"
     CDS             complement(7025..>7538)
                     /locus_tag="PK198_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40417.1"
                     /translation="MKDSTTVTVHGDPRLPISMADRKAKLSAYNEYKTVVEKATKGFE
                     QLKNARKSISLVNNALANTPDSVKNELVQLGKSMLDSISRLEKLYMMPDGLKGIQRSN
                     DDLSAVLFGASRYFNAFDGAPNANALNMMNQARRKTDAVLEEVNAFMNGDFAKYREKA
                     EVVKVEFFKE"
BASE COUNT         1644 a         1838 c         2042 g         1867 t
ORIGIN      
        1 tctccaccca ttttttcaat ttttttacaa ctgtctaaaa cagctttgtg ttcagttttc
       61 gttgtgatga tatgattacc ctttgaatga tacatttcaa aaacaccctt caatgcaagg
      121 ttatccgctt ctgtagcgcc agaagtgaaa ataatctctt tcgaatcgac gtgaattaaa
      181 tcagcaattt gttctcgggc ataatccaca gcttcttcag cttcccagcc aaaagggtga
      241 ttgcggctgg cggcattgcc cggtttttgg tagaaaaaag gcagcatggc atccactacg
      301 cgggggtcgg tgggcgtggt ggcgttgttg tccaagtata tccagttatt ttgcatcttc
      361 agtttattta gattgaatct aatatttaat tgagatcgca aaggtaacgg attgtcttgg
      421 aaaaagttcg agaaaaagga tgcgaaattt caaagccgct tcaaaaaagt attgattttc
      481 agaataattt cgttatccgg ttcaacacct taaaacctat gttttcggtc gccccggctg
      541 ggcgcccaat ggagcttcgc gtacatccaa agcgctgcaa gcagcgttgt atgaacaaaa
      601 tccgcctccg tatgatgcta tacgctgaaa gcgatattga ttttcacttc tcgaccgccg
      661 actgggccgt atgtcgctac gaccggcatc gctatttcaa aggctggagc gggcgcgggt
      721 tgaaaggcgt ggacttcatt gccgtgtggc gacaggacaa gctcatattc atggaggtga
      781 aaaactataa ccctcgtccc aacggcaaag gcgggcaaac agccgatgct gtcagagccg
      841 atccggccat actctccgat gcttttggag aaaaaattgc cgacaccctg accgccattg
      901 acgcggtgcg gcaataccgg caacgccgtt ggtggcgacg cttggcagcc tggtggtttc
      961 ggcggcgaaa ggtcgggagc agcgaaactg ctttttgggt acgcgtttgt gatttggcat
     1021 tgtctcctca aaactgcatg gcggtgcttt ggttggaagg agaggaattg gacgcctcat
     1081 tcagaaagac cattgaaaaa cggcttgcct acgagctttc ggcgctcacg gcacaggtgg
     1141 tggtctgcaa tgccgattcc aatcctttcc gcgaggacgg actgaccgca aagatagcag
     1201 aataaacaac ctcagccacc caccaatcca gccttcgctg aaatgtcgag catgcgatca
     1261 atgcaggccc ggccatcgtc tataatccat tgaggaagaa tgatttccgg ctgctcgtac
     1321 tccatacaca ggtagagttt ttccagcgta ttgagcttca tgtgcgggca gtcgttgcag
     1381 gcgcaggcat tgtcgggcgg agcaggaata aaggttttgt gcggcgatgc tttctgcatt
     1441 tggtgcaaaa tgccggattc agtagccaca atgtattcag tagccgcatc agagatggtg
     1501 tatttcagca ggccggtggt ggagcctaca tagtcggcca tttcgagaat gtgcggttcg
     1561 cattcggggt gggcaatgaa cttggcatcg gggtggcgtt ggcgcagttt gacgattttt
     1621 tcatggctga aaatttcgtg taccatgcag gcgccgttcc agagtaccat gtctcggccg
     1681 gttttttgta ccaaaaactt gcccagattg atgtcgggag caaaaataat gggctggtct
     1741 tcggggatgc tgttgatgat gtgcagcgca ttggtagatg tgcagcaaat atcggtgagg
     1801 gttttcagtt cggcggtaca gttgatgtag ctcacaacta catggccggg gtatttcttt
     1861 ttaaactccc tgaacaccgg cgcgggacag gaatcggcca gcgagcagcc tgccttgaga
     1921 tcgggcagca acactttttt atgcggagaa agcattttgg cagtttctgc catgaaatga
     1981 acgcctgcaa aaacgatgat gtctgcgtcg gtggcagcgg ctttttgaga caaccccaag
     2041 ctgtcgccta tgtaatcggc gatgtcttgt atgtcggcat cctgataata atgcgccaga
     2101 atgacggcat ttttttcgcg cttgaggcgg ttgatttctt gtaccagatc gagggtggga
     2161 tcaacgtcga gatcgaggaa tcctttgcgt tcgaggttgt tttgagctaa tgtgagcgta
     2221 ctcataagga tgtttttgtt gaggtaagta caacgccgga aggccggcaa atgttaagcc
     2281 caaaccgcag tggccggcag aaaaaaagcc ccgcctgatg gaatcagaca gggctgcgaa
     2341 taaagctatg aaaactaaaa ctatttcctc tttggtgaga gtcannnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnttc tccttcttcg gcgctcacat cgcggccacc ctgcaggtga
     2461 tcctgatatg cttcgagcgc ttcccactcg gcggtagcag ccaatgcggc ctgcttggcc
     2521 caggtagcgg gatcatgcat gcggtagcgc gggccggctt cgtccaaaac agcctgaacc
     2581 gtttcagtag cggcagatgc gagatcagcc caggttttga tgcctgcggc gtgcagcaat
     2641 tcctcaattt tcgggccgat gccttcgatg actttgaggt cgtcctgttt gatttttttg
     2701 ccggaaggca gagtgatgga agccgctttt gctgccggag cagccgccac ttcctctact
     2761 acgggagcag caatttcctc cactacggga ataggcgcag cctcgggttc gggggcggca
     2821 aaacattcta cgatgggagc ttctgtccga tgcgtatcag cagcgccaaa aggctgaatg
     2881 ctgataaacg tgcggttgag gcgcttgcgg cggaaaacca ccgtgccgtc cactgctgca
     2941 tggagcgtgt agtctttgcc cacatacacg ttttcaccgg catggaaccg ggtgccgcgc
     3001 tggcgaacga gaatgttgcc tgcgctgact gtctggccgc cgaacatctt tacgccaagt
     3061 cgtttggcat tactgtcgcg gccgttttgg gtactacctt cacctttctt atgtgccatg
     3121 attgtagttg tttcttggtt aatgaatgag tgccttttca ggccacgatg gaatcaattt
     3181 tgagcttggt aaaatgttgg cgatgacctt ttttgaggcg ataacccttg cggcgtttct
     3241 tcttgaaaat gatgaccttg tcgcctttga catggcccga aacaacggta gcgtttactt
     3301 tggctgcgtt caataccggc gtgcctacgc tcgtttggcc gtctcgttct accagaagca
     3361 cctggtcgaa actgagactg tcgccctctg cggcttctag ctggtggacg aagatctcct
     3421 gaccctcctc aactttgaac tgttgaccag ctattgttac gattgcgaac atcgaaagtt
     3481 ggatttgaaa tgatgaaaat tttgaatgag cgcgcaaagg taagcgcttt tttattttta
     3541 gccaaattct tatcaattgt ttttcagggc gctgtacaat ggctgaaaca aacgctgcaa
     3601 gaggttgcgc cgctcggtga tgatgtaggc tgttccctgc gattcgcgga gcaaaggcag
     3661 ggtgcgtccg tagttggttt tcaggccgga aggcagttca atgttgatgc gataagacgg
     3721 ggcgttgtcg gtcgaaacag gcgccagcgc aatatcgccc acataacccg tcagcacgcc
     3781 gtattccttg taggggaagc ccgcgaggcg aatgcgcaca ctcatatccg gggccacttt
     3841 tccggctcct gtggtaggca gcaagccctg ggcgcgaata ttaccttgcc cttcgggggc
     3901 caacaaagta gccacttcct gcccaggccg cagatactgc ccgagggtcc agttggaagc
     3961 cagcgacacg atgccgtcca ccggagccgt gactaaatag ttttgtttcc aaaaaaggat
     4021 ttcacttttc agattgcggg tcagttccct caaacgaatg tctgaactgc gcatgccgcc
     4081 gagctggctg aaccccatgt cggcaagttc tttgcgcaag ttgccgattt ttacctgatt
     4141 attgagttgt tcggtttgca gcgcttccac cttgcttttt ttgcctaaat atatttctct
     4201 gtctatttga aattcttgct ctttgatggc gccctgtttg tacttttcct ctgccttgct
     4261 gagccgggta gcggccaatt cttcctcttt tttagtcgtt tcctcctgct gtttcaggct
     4321 tgtgttgagc aattgcaagt ctttgatctg ctgccggagg gctgcaactc cctgaatgga
     4381 gtacagccgc cctttttcga gcataaattt ctccaatgct accaaccact cggaatacga
     4441 agactgcata ctgcccagtt cccagtctga caagaggtct tcggttgtcg gctcgtccat
     4501 atctacactg tccgacaaaa gcgcatccat tagtttttcc aatgcaagaa cgtgctccca
     4561 tttggccggg ttatcaacac aaatcaggac atcgccttca gccacctgat ccttttcttt
     4621 taccaaaaca ccggcaatgg ttccccccga aatactcact aaatgaatgg ccggccggtc
     4681 ggtgacaata ctgaccgggg ccgttactac atcgggataa cgaatgaacc acgccactac
     4741 aatagccaat aaggcgccgg ttaaagtaat gcctgtaccg cgccgaagta tccattcagg
     4801 cggcttgccc aggaggtatt gcacctctcg tagtaatatg tcttccggtt tgtcggtcat
     4861 aatcgaagat ggttgttcaa aactgcactt ttgtttgtaa agtcaactga gtgcgggccg
     4921 ggccggcaat ttcctccaaa cccgaaccaa aagttttctg gttggcccaa taggtctgag
     4981 caatgcgcgc ctctacggtg agtgcgcggg taggtttgta gcgcaaattg aagtaaaaac
     5041 gggtgccgcg attgtaatag gccggaannn nnnnnnnnnn nnnnnnnnnn nnnnnaggtt
     5101 gttttcaaac gcataaaaac gcacagcata accgtctgtg tcgaacagcg caaaacgggt
     5161 ggtaaacgac aacgggaatc ccagcggcct aaacagtaca tcctgataca gggctaagcc
     5221 attgctcatc gtgccggaac cgtcgtcgaa gcggcccaag tcgaggcggg tgcgcaactc
     5281 cagcatcttg ttcacctgat ggcctacgaa aaagcgcgcc tgcgtgagtt gcgttccggc
     5341 gaggtagtca atggcggtgg cattgttgcg gaagttgagt tccttgtttt cgcggcgaaa
     5401 ctcggcatag gccgtcagct tgcgcttgat gatgtgcgtg atgcggaggc gatactcgtg
     5461 cccgcgagag gggccgtcgg cgttgaattt gagccaggga tgctgccagg tatcgaagta
     5521 ggcattgatt ttccaaccgc gcatgggcct catttccagc ccgaagtaca aaccgtgttc
     5581 gttgcgtacg ccgccgcttt cgccgaaggc atcgccggtc agggtttgaa aatcgcgggc
     5641 ataatagcgg tgcaaaacgg ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnngagcgct gcgatggccc cgttgtcgct gatggcattt tctccgaaga agtggaaatt
     5761 gcgcaggatg aggccgtagt ccatcgaagc gttgaccaaa ctgttgccgc tgaaatagta
     5821 acgggcataa ggctgagtgc gcggtgctat gaacttatct aaagaagtat atactgcatt
     5881 gaatgccaag tgattgctgc cgggtttcca cttcagtgag gcgccggtag tgaactgcgt
     5941 gagggcgttt ttgtcggcta tttcggaagt cgtgcgatgc aggccgctga ggttgatgga
     6001 ggtgatttcc cggatttcgg ggtcaaagcc ttccacgtcg gtggtatcgg cttcnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacaactg cgccgccgca
     6121 ccccgctgaa aaacggcttc attgacggat gtataaggcc gcaacgcacg cccgccccgc
     6181 ttgaggccca ccgactgaat ggtcttgccg tagttgaaac cggtgagcat gatgaggcct
     6241 tgcccgaaag acacggcgta gtcgcccagc accacggctt tgatcgtacg gttgtaattg
     6301 cgcagcgcaa tgtgcgccga gtagaaatcg aagccgtagc gattggcgcc ggcaaagaac
     6361 tgctcgccgc ggtctttttc tgccgtaagg ccgatgctga gtttggttcc gtacgaatgc
     6421 cggaagcgca gatagtattg gttgggatcg ccgaggtagc ggctgtcggc agtatcggcg
     6481 gctgttgtga agcccttttg ttgttccagc accctggccc agcgcagata cagctcgttg
     6541 ctgccgtcgc ggagcatttc tttgatagac acctgatagt cgtccagatc gccgcccaca
     6601 gtgatgtaag gcatgagggc gcgaatgagt tcgggcgtga aaccgggtac tgcctgtagt
     6661 tcatacacgg agatgaggtc gccggcaatg gcgcgatggc gcagcaggcc cgctatttgt
     6721 acatcgttga gcaggagcag gtcgcggagg tcggcctcgt ctgctttgtt gatattgagc
     6781 ggtttgcgca ggtagtcgcc cagcgattca aacagcgtat tgaaatcgcc cacagcatct
     6841 gcatctgtat tttgcaggag gtcttccaac aagtcggtat tgccttcagc cggcgcagga
     6901 ggcagggtgt ccttttgggc gctcagggca agggcgcaac tcaggaggag cagtatggca
     6961 agcagacggc gcatggacaa aagtaaaaag gaaaaaggaa acccggcttg gttttatcca
     7021 aatttcactc cttaaaaaac tccaccttca ccacctccgc tttctcgcgg tatttggcaa
     7081 agtcgccgtt cataaatgca ttcacctctt ccagcacggc atccgttttg cggcgggcct
     7141 gattcatcat gttcaaggca ttggcattgg gagcgccgtc aaacgcattg aagtaccgcg
     7201 aagcgccgaa cagcactgcc gaaaggtcgt cgttagaacg ctggatgccc ttcagcccat
     7261 ccggcatcat gtacagtttt tcgaggcggg agatggagtc aagcatggat ttgccgagtt
     7321 gtaccaattc gtttttcact gaatcaggcg tattggccag cgcattgttg accaaactga
     7381 tggatttgcg ggcgtttttg agctgctcaa aacccttggt cgccttttcc accactgttt
     7441 tgtactcgtt gtaggcactc agtttagcct tgcggtcggc catagaaata gggaggcgcg
     7501 ggtcgccatg cacggtcacg gtggtcgaat ccttcatc
//
LOCUS       DAORLF010000227         7528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5306_length_7528_cov_1.742005, whole genome shotgun sequence.
ACCESSION   DAORLF010000227 DAORLF010000000
VERSION     DAORLF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_7528_cov_1.742005"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PK198_16620"
     CDS             complement(<1..494)
                     /locus_tag="PK198_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40418.1"
                     /translation="MKYWITILLAAVAMNASAQLTDTVEWEKLVQFESERKGPHLSAP
                     KNLNTLHYDVKYHRMEWEVSPLVRYIGGAVTTYFVPQTTGFNQIYFDLHTAMVVSAVQ
                     YRGTSCTFSQLPSSELLVTLPATLPVGVLDSLTISYGGVPPTTGFGSYATQNVCSNTV
                     PAMWT"
     assembly_gap    811..827
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<828..1305)
                     /locus_tag="PK198_16625"
     CDS             complement(<828..1305)
                     /locus_tag="PK198_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF40419.1"
                     /translation="MELATSTRLSHKAAEDYELVRSAVCGDQRAYSDLLKRYYPGVHQ
                     LMFKMVNNRHDAEDLTLEAFGKAFHNLSNYTPHYAFRTWLSRIAVNNCIDYKRKKRAR
                     LLSLDEPALSESGADLRNAIPCANPDPEEAFIRQQRSELLHRLSERLPQRHRQMIEM"
     gene            complement(1296..2420)
                     /locus_tag="PK198_16630"
     CDS             complement(1296..2420)
                     /locus_tag="PK198_16630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF40420.1"
                     /translation="MPTSETIQLLCLLLFGIATAVQVFFWALVFRLLARFRQPPAAGN
                     PSEEEFVSVIICARNEEEHLKKNLSHFLNQSYRSFEVIVVNDGSTDRTRNVVLDSMQK
                     WRNLRLVETQHDTPPGKKAALTLGIRAARGNYLLLSDADCRPASTEWISSMTAHTNTI
                     LEIGLGFGPYDRKRGLLNRFIRYETAYTAVQYFSLALQGLPYMGVGRNLLYKRSLFVQ
                     AGGFESHAHIASGDDDLFVNAVATKQNTAVVLDEKTFVFSEPKSTLRGYFKQKQRHVS
                     TGRSYKLLHQVLLGALSASHLLHYAAAAACWGVGAAPVWVLCGVLLRWAVVFLRWGAM
                     LRRLQQRDLLGWVIPFDLLYLLYYLTLTPALLVGNRNRWN"
     gene            2504..>3012
                     /locus_tag="PK198_16635"
     CDS             2504..>3012
                     /locus_tag="PK198_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF40421.1"
                     /translation="MKISTLQTGFFKLDGGAMFGIIPKRLWQKLNPPDENNLCTWAMR
                     CLLVQHEGRNILIDTGMGTKKGDKFLDFFEPHGEDSLMGSLQKHGLQPEDISDVFLTH
                     LHFDHAGGAVKWNEDGQAVPAFPNAVYWTNRLHYEWACHPNDREKASFIHDNFLPLEA
                     ADQLRFIPV"
     assembly_gap    3013..3203
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3440..3581
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3582..>4157
                     /locus_tag="PK198_16640"
     CDS             <3582..>4157
                     /locus_tag="PK198_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRF40422.1"
                     /translation="GAPPIHLADPNSALEVMTGAMLPTGANTVIRYEDVEIKDSVAHI
                     LTEDVKAGQNIHRQGHDRRAGDVIVPAGRRISPAEIGVAATVGKASLLAARLPKIAIL
                     YTGDELVDVETTPLPHQIRVSNAYSIQSLLAPWGIETQRIHLPDDLEATTAALASALQ
                     QFDALILSGGVSEGKFDYVPRALAGLGVQQLF"
     assembly_gap    4158..4265
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4284..4571
                     /locus_tag="PK198_16645"
     CDS             4284..4571
                     /locus_tag="PK198_16645"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40423.1"
                     /translation="MLPWLRQSLGLPAAPQERAALAEDFEFKPHLTYYLQVRVTCSET
                     GQLIARPLAGKGSGDLANLADADAFLELPQERSVFSAGEVFSLWRYRWLSH"
     gene            4624..5583
                     /locus_tag="PK198_16650"
     CDS             4624..5583
                     /locus_tag="PK198_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40424.1"
                     /translation="MLEHLSIKHFRLFEDFFLDGLRQVNLFTGKNNSGKTVLLEAIRI
                     WASQGDTTVINHILLQRGEWVPGWGESYEALFHRDNLSSHLSETDFSLSINEFSIVRH
                     TSENWKPYCESLWNGKRITPNLDASLPADFPRDGAVFLSLGADSQFPLFQLWNKIVLT
                     PEEDEVLEIIRETILPEIVRIDINQTRTLVRVSHEAKPLPLQNFGEGVLRMMLLAVAL
                     VSAKGKILLIDEVEAGLHHTTMEMFWKKIFDYAQKHNVQVFATTHSHDAVKAFTYALE
                     NTENTTSGTFIRLQKSRKNKSVETVKYSLDDLELSLESNLEPR"
     gene            5586..>6073
                     /locus_tag="PK198_16655"
     CDS             5586..>6073
                     /locus_tag="PK198_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40425.1"
                     /translation="MAQRLIVEGNDAIALAVLCRKSGLKPPVGYEGQSKFKNEFVIAA
                     GGINSALEKLRISLSEPELSHIGLIVDANDAGPLLTWQKIRNILSAFFASHTLSAADA
                     QTGAKVISEASMPTIGIWIMPDNTNQGYLEHFLSSMIPHNNELWRHADATLNHLNTTP
                     FN"
     assembly_gap    6074..6196
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6507..6535
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6536..>7528)
                     /locus_tag="PK198_16660"
     CDS             complement(<6536..>7528)
                     /locus_tag="PK198_16660"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40426.1"
                     /translation="DRIYNVTVTDPQLGCSVERQIAVRVHPAIELQTAAQVPVCEPGA
                     QAVLTASSTVAANYEWASNPLFVPVLGTGATFTTTPIAGASIYFVRATDSNGCGEVRE
                     VTVNNFPISATITPPVILCVPATTTALGVVNLAPAQTLTYSWSPANAVTPPTGSPVVT
                     VNAALAQNFSVILANQYGCLDTLSTTVTILDLPGTVSLNINPTEVLLGESSQLEVLGC
                     TGCSYQWSPAATLSDDDIRNPVATPTETTEYTVVVSSNGCSTTLRGTVTVTYLCEEPY
                     IFFPSAFTPNGDGENDELRLRARPPLVLEVNWVIYNRWGQKMFEAQNLNDSWDGT"
BASE COUNT         1696 a         1875 c         1739 g         1608 t
ORIGIN      
        1 gtccacatgg cgggcacggt attggaacag acgttctgcg tggcatacga gccgaagccg
       61 gtagttggcg gcacgcctcc ataactgatc gtcagggaat ccaatacgcc cacaggcagc
      121 gtagccggca gggtgaccag cagttcagac gagggcaatt gcgaaaaagt gcagcttgtg
      181 ccgcgatatt gcaccgcact cacaaccata gccgtgtgaa ggtcgaagta tatctgattg
      241 aaccccgtag tctgtggcac aaaatacgtc gtcactgcgc cgccgatgta ccgcaccaaa
      301 ggcgacacct cccactccat gcggtggtat ttcacgtcgt aatggagcgt gttgaggttc
      361 ttgggcgcac tgagatgcgg gccttttcgc tcggattcaa actgcacgag tttttcccac
      421 tctacggtat ctgtgagctg tgcagaagcg ttcatggcta ctgctgccag taggatcgtt
      481 atccaatatt tcataacaaa ttgatttaca ccgcaacatt acgaatttga ctaccgcgtg
      541 tcaatggcaa aaatcatccc gccctcttcc tgcaataaaa aaagccccct ttgcagacag
      601 gatgcaaaga aggctttcga gatttgatca tgatttgcta agaatacccc tttgaagtaa
      661 tgtggcgcga ggtatctaga taagcagaag cgcctggttt ttgcagcagc tcagaaaaca
      721 attctttggc acggaacaat tgagctttaa cggttccgag cgggatgttg agctcgacgg
      781 ccatttcgtc gtaactcttt tcctgaaaaa nnnnnnnnnn nnnnnnngca tttcgatcat
      841 ctgccggtgg cgttgcggca ggcgctccga caaacggtgg agcaactccg aacgctgttg
      901 gcgaatgaag gcttcttcag ggtcaggatt ggcgcaggga atggcgttgc gcaggtcggc
      961 accggactcc gaaagggccg gctcatctaa tgacagcagt ctcgcgcgtt tttttctttt
     1021 gtaatcaatg cagttgttca cagcgatgcg agaaagccag gtacggaatg cgtagtgggg
     1081 ggtgtagttg gacaaattgt ggaacgcctt gccgaacgcc tccagagtga ggtcttcagc
     1141 atcatggcgg ttgttcacca ttttgaacat caactgatgt acgccggggt aataacgctt
     1201 cagcaaatcg gaataagccc gctgatcgcc gcacacagca gagcgaacca gttcgtaatc
     1261 ttcagcagcc ttgtgagaca gacgtgtaga agttgctaat tccatcggtt tcggttgccg
     1321 accaaaagcg ccggcgttaa agtcagataa tacaataaat acagcaggtc gaacgggatg
     1381 acccagccca gcagatcgcg ctgttgcaat cggcgcaaca tggcccccca acgaaggaaa
     1441 accaccgccc aacgcagcaa cacgccgcac agcacccaaa ccggcgccgc gcctactccc
     1501 caacaagccg ccgctgccgc gtaatgcaac aaatggcttg ccgacaaagc tcccaatagt
     1561 acctgatgga gcagcttgta gctcctccct gtgctcacgt gccgttgttt ttgtttaaaa
     1621 tagcctctca aagtgctctt cggctccgaa aacacaaaag ttttttcatc caacactact
     1681 gccgtgtttt gcttcgtagc aacggcattt acaaacaggt cgtcatcgcc cgaagcgatg
     1741 tgagcatggc tttcaaaacc gccggcctgc acgaacaaac tgcgcttgta cagcaggttg
     1801 cggcctacgc ccatatacgg cagcccctgc aatgccagcg agaaatactg caccgcagtg
     1861 taggctgttt cgtaccggat aaagcggttg agaagtcctc ttttccggtc gtagggacca
     1921 aagcccagac ctatttccaa tatagtgttg gtgtgtgccg tcatgctgct gatccattcc
     1981 gtagaagcgg gtcggcagtc ggcatcgctc agtagaagat agttgcctcg ggcagcgcga
     2041 atgccaagcg tcagcgctgc tttttttccg ggcggtgtgt catgttgcgt ttcaacgagg
     2101 cgcaagttcc gccatttctg catactgtcc aacactacgt ttcgggtacg gtcggtagaa
     2161 ccgtcattta cgacgatgac ctcaaaggaa cggtaggatt gatttagaaa atggctgaga
     2221 ttttttttga gatgctcctc ctcgttgcgg gcgcagatga tgacggagac gaattcctcc
     2281 tccgaagggt tcccagcggc agggggctgc cgaaaacgcg ccaggagacg aaacaccagc
     2341 gcccaaaaga aaacttgtac agccgtggcg ataccgaaca gcagcaggca taaaagttgt
     2401 atggtttcgc ttgtcggcat gttctgtcat ttgggcgttt gaaaaaaaaa atcatcttta
     2461 cagccacgat tcacagacgc agaagcgcca aaatacttat cttatgaaaa tcagcacatt
     2521 gcaaaccggc tttttcaaat tggacggtgg cgccatgttc ggcattatcc ccaaacgctt
     2581 gtggcaaaag ctcaatccgc ccgatgagaa caacctctgc acctgggcca tgcgttgcct
     2641 cttggtacaa cacgagggcc gaaatatcct catagacacc ggaatgggca ccaaaaaagg
     2701 cgataaattt ctcgattttt ttgaacctca cggcgaagac tccctcatgg ggtcgcttca
     2761 aaaacatggc ctccaacccg aagacatcag cgatgttttt ctcacgcacc tccatttcga
     2821 tcatgcgggc ggcgctgtca aatggaacga agacgggcag gctgtgccgg ctttccccaa
     2881 tgccgtctat tggaccaacc gcctgcacta cgagtgggcc tgccacccca acgaccgcga
     2941 gaaagcctcc ttcattcacg acaacttcct cccgctggag gccgccgacc aactccgctt
     3001 cattcctgtg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnctgctcg aaaaggaaaa gctgttggcc gaagccgcag
     3241 atcgtgggta ccgccttatt tttgaacacg atccccgcac cgccgcctgc acccttgttc
     3301 gcaacgagca tggccgtatt catgcaggtg aaaacatctc catttaatta tgatctccgc
     3361 ttccgaagcc ttccagatcg tcctccaaca tgccgcttcg ttcggcgacg aacacatacc
     3421 gctcgaccgc gcattgggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgctccg ccgattcatc
     3601 tcgccgaccc caattcggca ttggaagtga tgaccggcgc catgctgccc actggcgcca
     3661 atactgtcat tcgttatgaa gatgtagaaa tcaaagacag tgtggcgcat attctcaccg
     3721 aggatgtgaa ggcagggcaa aacatacacc ggcagggcca cgaccgccgt gccggcgatg
     3781 tcattgtacc ggcaggccgc cgtatcagtc cggcagaaat cggcgtagcg gctacggtgg
     3841 gcaaagcctc gctgttggca gcccgcttgc cgaagatcgc cattctttac accggcgacg
     3901 aactggttga tgtggagacc actcccctac cccatcaaat cagggtatcc aatgcgtaca
     3961 gcatccagtc tttgctggcg ccctggggca tagagacaca acgcattcac ctgcccgacg
     4021 atctggaggc caccactgcg gcgctggcat ccgcattaca acagtttgac gcgctcatcc
     4081 tcagcggcgg ggtgtcggag ggtaagttcg actatgtgcc ccgcgcattg gcaggtttgg
     4141 gcgtacagca gttgtttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnngccgg catgatgcgc taggtgctgc cctggctccg gcaatcgctg ggactgcccg
     4321 ctgccccgca ggaacgcgcc gcactggccg aagacttcga gttcaaaccc catctgacgt
     4381 attaccttca ggtgcgtgtg acctgctcgg aaacggggca actgatcgcc cggccattgg
     4441 caggcaaagg ctccggcgac ttggccaatc tggccgatgc ggatgcgttt ctggaattgc
     4501 cgcaggagcg cagcgttttc agcgccgggg aggtgttttc attgtggagg tataggtggt
     4561 tatcgcattg attttgagct tatattactt atttttcaat gagtttttct atttttgcac
     4621 tctatgcttg agcatctctc tataaaacac tttcgcttat tcgaggactt cttccttgac
     4681 ggtcttcgtc aggtcaattt gtttacagga aaaaacaatt caggcaagac cgtattgctg
     4741 gaagctataa gaatatgggc atcacaggga gatactaccg ttatcaacca tattttactc
     4801 caaaggggcg aatgggttcc ggggtggggc gagtcttacg aggctctttt tcatcgagac
     4861 aacttgtcaa gtcatctctc ggagactgat ttttcattat caatcaatga gttctccatc
     4921 gttcggcata cctccgaaaa ctggaaaccc tattgtgagt ctttatggaa cggaaaaagg
     4981 attaccccta acttggatgc gtccctgcct gccgactttc ccagggacgg ggcagttttc
     5041 ttgtctctcg gcgccgacag ccagtttcct ctttttcaac tttggaataa aattgtactg
     5101 acaccggaag aagatgaagt actggaaatc atacgggaaa ccatcctccc tgaaattgta
     5161 cgaattgaca tcaatcagac gcgtactttg gtacgggtta gccacgaagc caaaccattg
     5221 ccgttacaaa actttggaga aggcgttctt cgcatgatgc tgctggcggt ggcgctggtc
     5281 agtgcaaaag gcaaaatact tcttattgat gaagtagaag ccgggctaca tcataccacc
     5341 atggagatgt tttggaaaaa aatatttgac tacgctcaaa aacacaatgt gcaggttttt
     5401 gctactacac atagccatga tgccgtcaaa gccttcacct atgctttaga aaatacagag
     5461 aataccacat caggcacttt tatccgatta cagaaaagcc gaaagaacaa gtcagtagaa
     5521 accgtaaaat attctctgga cgacctcgaa cttagccttg aatccaatct cgaacctcgt
     5581 taatcatggc ccaaagattg attgtagaag gaaacgacgc cattgcattg gctgtgctat
     5641 gccgaaaatc aggcttaaag ccacccgtag gatatgaggg gcaaagcaag ttcaaaaatg
     5701 aattcgtgat agcagcagga ggtatcaata gtgccctcga aaaactcaga atttcactct
     5761 cggaacctga actcagtcac atcggattga tcgttgatgc caatgatgcc ggccccctgc
     5821 ttacatggca aaaaatcaga aacatcctgt ccgctttttt tgcctcccat acattatctg
     5881 ctgccgacgc acaaacagga gccaaggtca tctcagaagc ctctatgcct accatcggca
     5941 tttggatcat gcccgacaac acaaatcagg gatatctgga gcattttctt tcatctatga
     6001 ttcctcataa taatgagctt tggcgccatg ctgatgccac gctaaaccac ctgaatacca
     6061 cgccattcaa tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnngatc gagccttttc tggaatggtt caccaatacc tttgagttgg
     6241 ggcattgaat tccggcccca tgctacaaaa aaaacggcgc cccgatcttt catcaggacg
     6301 ccgttccttc aattcatttc tcaacaataa gtgccagagc taaatcaatt gttgttatta
     6361 tggctgctcg caattggcaa tcagcacatt agcacattat aaatcagcac attttcttat
     6421 cacctcaaca gcgtcacgtt gccttttttg gtaaacgtct cgccgccggg acagctcacc
     6481 gtcaggtaga aaccataaac atcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgcc
     6541 gtcccaggaa tcgttcaggt tctgggcttc gaacatcttt tgcccccagc gattgtaaat
     6601 cacccagttc acttctaaaa caagcggcgg gcgggcgcgc aaacgcaatt cgtcgttctc
     6661 tccgtcgccg ttcggcgtga aggccgaggg gaagaagatg tacggttctt cgcacaggta
     6721 agtcaccgtc accgtgccgc gcagcgtagt gctgcagcca ttgctgctca ccactacagt
     6781 gtattccgtt gtctcagtag gcgtggctac cgggttgcga atatcgtcgt cgctcaacgt
     6841 agctgccggc gaccactgat aggaacagcc cgtacaaccc agcacctcca actgcgaaga
     6901 ctcgcccagc agcacctccg tcgggttgat gttcaggctc actgtacccg gcaggtcgag
     6961 gatggtcacc gtagtactca gtgtatccaa acagccatac tggttggcta atatcacact
     7021 aaagttttgg gccagcgctg cattcaccgt cacaaccggg ctaccggtgg ggggcgtcac
     7081 ggcattggcc ggcgaccagg agtaagtcag cgtttgtgcc ggcgccaggt tgacaacacc
     7141 caaagcggtc gtcgtagcag gtacacacaa gatcaccggc ggcgttatgg tggccgaaat
     7201 cgggaagtta ttgaccgtta cttcgcgcac ttcgccacac ccgttgctat cggtggcgcg
     7261 cacgaaatag atactcgctc cggctatcgg cgttgtggta aaagtagcgc ctgtacccaa
     7321 taccggcaca aacaacggat tggaggccca ctcataattg gcggccactg tgctggaggc
     7381 cgtcaataca gcttgtgcgc cgggttcgca aacaggcacc tgagcggctg tttgtaattc
     7441 tattgcagga tgcacccgaa ccgctatttg cctttctaca ctgcatccca actgcgggtc
     7501 ggtgaccgtg acgttgtaga tgcggtcc
//
LOCUS       DAORLF010000228         7498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5337_length_7498_cov_2.275561, whole genome shotgun sequence.
ACCESSION   DAORLF010000228 DAORLF010000000
VERSION     DAORLF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5337_length_7498_cov_2.275561"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>288)
                     /locus_tag="PK198_16665"
     CDS             complement(<1..>288)
                     /locus_tag="PK198_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40427.1"
                     /translation="MKVVMLYYLTPLPNLGFSASSDNVMSMLPPQLRPGAPQSQALTN
                     YVKNGGHLFIAGDPTPFIHVTGRIPADYSVPPSPGNYQYTEYGCAGAGGCV"
     assembly_gap    289..304
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<305..1328)
                     /locus_tag="PK198_16670"
     CDS             complement(<305..1328)
                     /locus_tag="PK198_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40428.1"
                     /translation="MKRLLNQAKLSYLLVLGLAGLMLHSCDQDPKFSDLVPDYAYSII
                     RSFEANGELAEINHTNAVVTHTLPAGADLATVNVNMTLPEGAVVQPASGSTVDFSGGP
                     VVFTVTNNGIARKYTVTIAAFGDPMIMTFAIGSNMGVIDQANGAIAITVGSQADITRL
                     APLYTIPGGTTATPESGVAQNFTNPVKYTVISNDGFTGKSYFATVTQIEGPKITKFEV
                     NGEEATIFNSTRMISLTLPPGTNLSNLAPVITLPPGQTISPAGGVARDFSAGPLTYTV
                     TNTEGLTAQYTVNITAGSLPIAFIGDGANVNSIQDDDARAAALFLQATYQNDFHYIPF
                     SNISAQT"
     gene            complement(1361..3058)
                     /locus_tag="PK198_16675"
     CDS             complement(1361..3058)
                     /locus_tag="PK198_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF40429.1"
                     /translation="MKNLSSIPAILLSLFLLAGCSKDFLDVQPENVISEENLDIEQLV
                     VNAYGALDYRYNTGEFRDHWPFDHAPSNWAFSDIRSGDAYKGGGGVGDNPGGGMHALE
                     IHQVFPSSENVYNLWRALYFAIFRVNNAIRSLNAATGFDKKELRIAELRVLRAHFYFE
                     LMKNFGTFAYVSDQTPVDQMAQVPNSFDQAFLWGKIEEDLLASIPILPETQPELGRVN
                     KLVAYAYLAKAKLFQKQWSEVIAHADRVLAGPYSLAQDIERLYSIPGYGNSENVFAIQ
                     YSINDGSEYGNLNFGNLLNSPDSPADDVNHPYLNGDDFHKPSQNLVNAFKVDADGLPL
                     LDTYNNADLAPRDVTTPVDPRLDHAVGRPGIKWKSWNRMAQQDNWSRDVPTYGYYVLK
                     KNQIDPNSDQRASGGFPWAKGALDFPLIKISDVMLWKAEAAIELGNTATGMSIINQIR
                     ERAKNSPYVRDFIDDTRLAANYQINLYPATVSQDYARKALRMERRLELHNEGHHFYDL
                     VRWGIASQWINNYMAAESAKRTYYAGASFTPNRDEYLPVPQIEIDASGGVYVQRPNY"
     gene            complement(3080..6172)
                     /locus_tag="PK198_16680"
     CDS             complement(3080..6172)
                     /locus_tag="PK198_16680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40430.1"
                     /translation="MKMKLISLFVLFFSAQFALAQRTVTGKVTDVETNEPLIGANVLV
                     KGTSIGTTTEVDGSFVLVVQDNAASLTISYTGYQPAEVSIVGVQNVNVALTTGSLLET
                     VVVTGYTTQRKREITGAISIVDAADLADVATPNVITKLQSKVPGLTFTSSGVPGGNDT
                     QISIRGLTSVFGGTGPLWVIDGVQTTNPSGLNPNEIESIQVLKDAASAGIYGTEAARG
                     VIIVTTRQARKGASGFTFNTQVTSNRVRENFSVLGAQEWLDVRYLAQGKTPVTAGNFS
                     YTPGTELPEFLDAGKNLRLSNTDWVDVILRNSISTTTDLGYSIAKDKWRLFAGAGYAG
                     DNGIIEHTYYRRGNLRLNSSVKLFGDFLTIGENLTVSQFNEVKNGGDNAPVVEDALLQ
                     NPLIPVYGEDGSWGGPTGAGLQDKWNPLAILYINRNNVQKTLRTFGNVYADFALTPHL
                     SFSSKYNFDYNNFKYDEKTEFFNQNGSLLGNRILLNGQDFSRFARNRNNAATYIWTNL
                     LTYGRDFGNHTINAFVGQEVFERKQDHVFHRIQVPVGTEIDYDNIEQYDVIADNSILD
                     AYGVGADSRRSSLFAKASYDFADKYFVSASLRRDGSSRFGRNNRYAIFPTVSLGWTLS
                     REAFLANNKNINDLKLRFSWGGNGNADIAEYAQYSIFISAIEYSNYDLAGTGSGNIAT
                     GVSASQVGNPDLKWEQSYQTNLGLDMTLFDYKVNFSMDVYEKNTTDLLLQTVQPSVLG
                     EAGKTLFYNAGDMVNRGIDLVLGYQSNRKSDFNYGFNVLFSAYRNKVTSLNNSEDFIL
                     EGVSYTGVGYAIGSYFGFVADGIFRTPEEVAVHAAQPGKALGNIRYRDLNGDGVIDQE
                     DRTIIGNPHPDFTYGIGANMSYKQWGLSLFFDGRHGNDMYNAQRNLLDFPYFGFNHGR
                     NTLDAWRPDNANSLIPALNTADINNQTRPSTYFVEDGSFFRLKTISLSYTLPGASLGR
                     MGLRNARIYVQGENLINLTNFTGFDYEVRGLSRTGIGIAGLNLYPHTRSMTVGLSLDF
                     "
     gene            complement(6244..>6703)
                     /locus_tag="PK198_16685"
     CDS             complement(6244..>6703)
                     /locus_tag="PK198_16685"
                     /inference="COORDINATES: protein motif:HMM:NF019849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HRF40431.1"
                     /translation="SAELSGFLDITAQIGATSPAQMELVLDFELPAGAYPDFGVQLSN
                     SKGEQYRVGYNAAAKQFYSDRRNTGNISFSDKFARSNHSAPRLSEEKTIRMHLFLDAA
                     SAELFADGGATVLTDIFFPGEDFHQIALYSEKGSVKLLKAEAFRLRSIWR"
     assembly_gap    6704..6729
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6730..>7078)
                     /locus_tag="PK198_16690"
     CDS             complement(<6730..>7078)
                     /locus_tag="PK198_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRF40432.1"
                     /translation="AANGGSGTQYFVGNFDGKKLELDPTFAPDVSGEKAVWLDYGRDN
                     YAGVTWSDIPKEDGRRIFIGWMSNWDYATRVPTQKWRSSTTIPRTLTLRKTGEGYRVF
                     SEPVKELQNLRASA"
     assembly_gap    7079..7113
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7114..7470)
                     /locus_tag="PK198_16695"
     CDS             complement(<7114..7470)
                     /locus_tag="PK198_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRF40433.1"
                     /translation="MAIFTYHNPDAERAGRSDFQYQGIAYSNDKGRTWTKYPGNPVVP
                     NTQKIRDFRDPKVIWDEASQQWVMVFAAADHVKLWRSDNLTEWTHLSDFGKEWGSHGG
                     VWECPDLFPIQVEGSNE"
BASE COUNT         1608 a         1852 c         2128 g         1833 t
ORIGIN      
        1 ccacgcaacc tcctgcgccg gcacaaccat attctgtgta ttgataattg ccggggctgg
       61 gaggtacgga gtaatcggca gggatgcgcc cggtgacatg gatgaacggc gtggggtcgc
      121 ccgcaataaa gaggtggcca ccgtttttga cataattcgt cagcgcctga ctttgggggg
      181 cgcccggtcg aagttgaggc ggcaacatgc tcatcacatt gtcggacgat gccgaaaagc
      241 caagattggg aagcggtgtc aggtaataca acatgactac tttcatgcnn nnnnnnnnnn
      301 nnnnatgtct gtgcagaaat attggagaat ggaatgtagt gaaagtcatt ttggtaggta
      361 gcctgcaaga aaagcgctgc ggcgcgcgcg tcgtcatcct gaatggaatt tacattggcg
      421 ccgtcgccga tgaatgcaat gggcaggctt ccggctgtaa tgttcaccgt gtattgtgcc
      481 gtaagtcctt ctgtatttgt gacggtgtaa gtgagcgggc ctgctgagaa atcgcgggct
      541 accccgccgg ctggagagat ggtctggccg ggaggtaaag tgatgacggg cgcgagattg
      601 ctcaggttgg ttccgggagg caatgtcagc gaaatcatgc gcgtgctgtt gaagatggtg
      661 gcctcctcgc cgttgacttc aaacttcgtg attttgggcc cttcaatttg agtcaccgtc
      721 gcaaaataag actttcctgt aaagccgtca ttagagatga ccgtgtactt gacaggattg
      781 gtgaagtttt gggctacgcc cgattctggt gtggcagtag tgccgccggg gatggtgtac
      841 agcggcgcaa gccgggttat atccgcctga ctgccgacgg tgatggcaat ggcgccattg
      901 gcctggtcaa taacgcccat gttgctgcca atggcgaagg tcatgatcat cgggtcgcca
      961 aaagcagcga tcgtgacggt atattttctg gctataccat tgttggtgac cgtaaaaacg
     1021 acagggcctc cggaaaagtc aacggttgaa ccggaggcag gctgtacaac ggctccttcg
     1081 ggcagggtca tattcacgtt tacggtggca agatcggcgc cggcaggaag cgtgtgcgtc
     1141 actacggcgt tggtgtggtt gatttcggcc aactcgccat tggcttcaaa actcctgatg
     1201 atggagtacg catagtctgg tacaagatct gagaatttgg gatcctgatc gcagctatgc
     1261 agcatcaagc cggccagccc caatacaagc aggtagctca gcttcgcctg gtttaacaat
     1321 cgtttcatat caagtttggt taaagtttga aatatttgaa tcaataattc gggcgttgca
     1381 catatacgcc gccggatgcg tctatctcta tctgcggcac cggcaggtac tcatcccggt
     1441 tgggcgtaaa ggaagcgcct gcataatagg ttcgcttagc gctttctgca gccatatagt
     1501 tgttgatcca ttggcttgct attccccagc gaacgagatc gtagaagtga tggccttcgt
     1561 tgtgcaattc caggcggcgc tccatgcgca gggctttgcg cgcatagtcc tggctgaccg
     1621 tagcagggta caaattgatt tggtagttcg ctgcaagacg cgtgtcatct atgaagtcgc
     1681 gtacataagg cgaatttttg gcgcgctcgc gaatctggtt gatgatgctc attccggtag
     1741 ccgtgttgcc cagttcaatt gcagcttctg ccttccacag catgacgtcg ctgatcttga
     1801 tgagcggaaa atccaaagcg ccctttgccc aggggaaccc tccggaggcc cgctggtcgg
     1861 agttgggatc aatctggttt ttcttcagca catagtagcc ataggttggc acgtcccggc
     1921 tccagttgtc ttgctgcgcc atccggttcc aggacttcca cttgatgccc gggcggccaa
     1981 cggcatgatc caggcggggg tccacggggg ttgtcacatc gcggggcgcc agatcagcgt
     2041 tgttgtaagt gtccagcaag ggcaagccgt cggcatctac cttgaaggca ttgacaagat
     2101 tctgagacgg cttgtggaaa tcatccccat tgagatacgg atgattcaca tcgtcggcgg
     2161 gcgagtccgg cgagttcagc aaattgccga aattgagatt gccgtattca gaaccgtcgt
     2221 tgatggagta ctggatggca aaaacgttct cactgttgcc atatcccgga atggaataaa
     2281 gccgctctat atcttgcgcc aggctatatg gccctgccag cacccggtct gcatgcgcta
     2341 tcacttcgct ccattgcttt tggaagagtt tggccttggc caaataagca taagctacca
     2401 atttgttgac gcggccaagc tcaggttggg tttcgggtaa aatgggaatg gatgccagga
     2461 ggtcttcttc aattttgccc cacaggaaag cctgatcaaa agaattgggt acttgcgcca
     2521 tctggtccac aggcgtctga tcgctgacat aggcgaaagt gccgaaattc ttcatcagct
     2581 caaagtagaa atgcgctcgc agtacgcgca attcggcgat ccgcagttct tttttgtcaa
     2641 agccggtagc ggcattcagg ctccggatgg cgttgttgac gcggaaaatg gcaaaatata
     2701 gtgccctcca caggttgtac acattttcgg aagatgggaa cacctgatgt atttccagcg
     2761 cgtgcatgcc gccgccggga ttgtcgccca cgccgccgcc gcctttgtag gcatcgccgc
     2821 tgcggatgtc tgaaaacgcc cagttggagg gcgcatggtc aaatggccag tggtcgcgga
     2881 attcgccggt gttgtaccgg tagtcgagcg ctccgtaggc attcactacc aactgctcta
     2941 tatccagatt ctcttccgaa atgacatttt cgggttgtac gtccaaaaaa tctttggagc
     3001 acccggccag caggaagagt gagagcagta tcgccggaat agatgataaa tttttcataa
     3061 tcattgctgg ttttttttgt tagaaatcca ggctcagacc aacggtcatg gagcgggtgt
     3121 gcggatacag attcagccct gcaataccga tgcctgtgcg gctcaacccc cgcacctcat
     3181 aatcgaatcc tgtaaaattg gtcaggttga tcaggttctc gccctgcaca tagatgcgcg
     3241 cattgcgcaa gcccatgcgg cctagcgacg cgcccggcag tgtgtagctc aggctgatgg
     3301 ttttgaggcg gaagaaagag ccgtcttcta caaaataggt agaggggcgc gtctggttgt
     3361 tgatgtcggc cgtgttcagc gcaggtatca gagaattggc attgtcgggc ctccaggcgt
     3421 caagggtgtt tcgaccgtgg ttgaagccga aataggggaa gtcgagcagg ttgcgctggg
     3481 cattgtacat gtcgttgccg tggcgcccgt cgaaaaacag gctcaatccc cattgtttgt
     3541 agctcatatt ggcgccgata ccataggtaa aatccgggtg cggattgccg atgatggttc
     3601 ggtcttcctg gtctatgacg ccgtcgccgt tcaggtcgcg gtagcgtatg ttgccaagcg
     3661 ccttgccggg ttgggcggcg tgaacggcca cttcttccgg cgttctgaat atgccgtccg
     3721 ctacgaagcc gaaatacgag cctatggcgt agccgacgcc ggtgtaagac acgccttcca
     3781 ggatgaaatc ttccgaatta ttcagcgatg tcaccttgtt gcggtaagca gaaaacagta
     3841 cattgaatcc gtaattgaaa tccgacttgc ggttggactg gtagccgagc acgaggtcaa
     3901 tacctctgtt caccatgtcg ccggcattgt aaaagagcgt ttttcctgcc tcgcccaaca
     3961 cggatggctg cacggtttgc aaaagcaggt cggtggtgtt cttctcataa acatccattg
     4021 aaaaattgac cttgtagtca aacagggtca tgtccagtcc cagattggtt tggtagctct
     4081 gctcccattt caaatccgga ttgcccacct gactggccga gacgccggtg gcgatgtttc
     4141 cgctgcctgt tccggcgagg tcatagttgc tgtattcaat agccgaaatg aagatgctgt
     4201 actgggcata ctcggcgatg tctgcgttgc cgttgccccc ccaggagaac cggagtttca
     4261 agtcatttat gtttttgttg ttggccagaa aggcttcccg gctcagtgtc caacccaaag
     4321 aaacggtcgg aaaaatggca tagcgattgt tgcgcccgaa gcgggaggag ccgtcgcggc
     4381 gcaaactggc agaaacaaag tatttgtcgg cgaaatcata ggaagctttg gcaaagagcg
     4441 aactgcggcg gctgtcggcg cctacgccat acgcgtccag aatggaattg tcggcaatga
     4501 cgtcgtattg ctcaatgttg tcataatcaa tctccgtgcc cacgggcact tgtatccggt
     4561 gaaagacatg atcctgcttt cgctcaaata cttcctggcc tacaaaggca ttgatggtgt
     4621 ggttgccgaa gtcccttcca taggtcaata ggttggtcca tatataggtg gcggcattgt
     4681 tgcggtttcg ggcaaaacgg gaaaaatcct ggccattgag cagtatcctg ttgccgagca
     4741 gacttccatt ttgattgaag aactccgttt tttcgtcgta tttgaaattg ttgtaatcaa
     4801 aattgtactt ggagctgaaa gacaaatggg gcgtgagcgc aaaatcggca tacacgttgc
     4861 caaaagtgcg gagggttttc tggacgttgt tgcggttgat gtacaggatg gccagcgggt
     4921 tccacttgtc ctgcagacca gcgccggtgg ggcctcccca ggagccgtct tctccgtaaa
     4981 cggggatgag cgggttttgc agcagggcat cttcgaccac aggcgcattg tcgcctccat
     5041 ttttaacctc attgaattgc gagacagtca gattttctcc gatggtcaaa aaatcgccga
     5101 acagcttcac gcttgagttc aggcgcaagt ttccacggcg gtagtaggta tgttcaatga
     5161 tgccgttgtc gccggcataa cctgctccgg caaagagcct ccacttgtct ttggcaatcg
     5221 aataacccag atcggtggtg gtagaaatgg aattgcgcaa aatcacatcc acccagtctg
     5281 tgttggaaag acggaggttt ttgcccgcat ccagaaattc gggcagttcc gtacccggtg
     5341 tgtaagagaa gttgcctgca gtcaccgggg tcttaccctg tgccagatag cgaacatcca
     5401 gccactcctg agcgcccagt acgctgaaat tttcgcgcac tctgttggaa gtcacctgtg
     5461 tattgaaggt aaagccgctt gctcccttcc gggcttgcct ggtagtgaca atgatgacgc
     5521 cgcgcgccgc ctctgtaccg tagatacctg ccgaggctgc gtccttgagc acctgaatgc
     5581 tttcaatttc gttcggattc aatcccgaag gattggtggt ctgtacgccg tctatgaccc
     5641 acagcgggcc ggtgcccccg aatacggagg tgagcccccg gatggaaatt tgggtgtcgt
     5701 tgccgccggg cacccccgaa gaggtgaagg tcaggccggg tactttcgat tgtaattttg
     5761 taatgacgtt cggggtggcc acatcggcaa ggtcggcggc atccacgatg gagatagcgc
     5821 cggtgatttc cctttttcgc tgggtggtgt acccggttac gacaacggtt tcaagcaaac
     5881 tacccgtcgt gagggccacg ttcacgtttt gtacgcccac aatagacacc tctgccggct
     5941 ggtagcctgt gtaggaaatg gtgagcgaag cggcgttgtc ttgtactaca aggacaaatg
     6001 agccgtccac ttcagtagtg gtaccgatgg aggtgccttt caccagtacg ttggcgccga
     6061 tgagcggttc gttcgtttct acgtctgtta cttttcccgt cacagttcgc tgggccagag
     6121 cgaattgcgc cgaaaaaaac agcacaaaga gcgagatcaa cttcattttc atacatttat
     6181 ggtattaatg actgattgaa taggcagatg ttccgctcag gcggacatct gcattttgtt
     6241 cggctatcgc cagatggagc ggagccggaa agcctccgcc tttaatagtt ttacgctgcc
     6301 tttctcggaa tacagtgcga tctgatggaa gtcttcgccg ggaaagaaga tatctgtcaa
     6361 tacggtagcg ccgccgtcgg caaagagttc ggcggaagcg gcatccagaa acaggtgcat
     6421 ccggatggtc ttttcttctg aaagccgggg cgcggagtga ttgcttcggg caaatttgtc
     6481 ggagaaggat atattgcctg tatttctgcg gtcgctgtaa aactgttttg cagcggcatt
     6541 gtaccctacg cggtattgct cgcccttgct gttggaaagt tgtacgccaa agtcggggta
     6601 ggcgccggcg ggtagttcaa agtccaggac cagttccatt tgcgccggcg aggtagcccc
     6661 gatttgtgct gtgatatcca agaaacctga cagttcagcg cttnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnng agccgaagcg cgcaagtttt gcagttcttt cacaggctcc gagaacacgc
     6781 ggtagccttc accggtcttc cgaagtgtga gcgttcgtgg aatggtagtc gagctgcgcc
     6841 atttttgagt gggtacgcgg gtggcgtagt cccagttgct catccagccg ataaaaatgc
     6901 ggcggccgtc ttctttggga atatccgacc aggtgacccc ggcatagttg tcacggccat
     6961 agtcgagcca gacagctttt tctccgctca cgtcgggagc gaatgtgggg tccaactcca
     7021 attttttacc gtcaaaattt cctacgaaat attgtgtgcc ggagccgccg ttggcagcnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcgttg ctgccctcta cctgaatagg
     7141 aaacaaatcc gggcattccc acacgccgcc gtgtgatccc cactccttgc cgaaatcgct
     7201 gaggtgcgtc cactcggtca gattatccga tctccatagt tttacatggt cggcggcggc
     7261 aaataccatc acccactgtt gggaggcttc atcccagatc actttggggt cccggaaatc
     7321 tctgattttc tgagtgttgg gcactaccgg attgccggga tatttggtcc aggtgcggcc
     7381 tttgtcgttg ctgtacgcga tgccctgata ttgaaaatcg gagcgaccgg cgcgttctgc
     7441 atccgggttg tgataggtaa agatggccac caacggcggc cgaccgtttt ggcccaat
//
LOCUS       DAORLF010000229         7492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5341_length_7492_cov_2.208148, whole genome shotgun sequence.
ACCESSION   DAORLF010000229 DAORLF010000000
VERSION     DAORLF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_7492_cov_2.208148"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PK198_16700"
     CDS             complement(<1..696)
                     /locus_tag="PK198_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF40434.1"
                     /translation="MKIQIISDLHLEFGEGETNFEQADVVILAGDTHVGTKGVEWVKA
                     KIPDKPVIYLLGNHEYYNGSYPKTLHQITDAAKDTNVLVLENKRIEIEGITFHGATLW
                     TDFALYGNPFLYGTICQLRVNDFKRIRRDPSYSKMRSVDIAHIHQHSKFWLAASLEES
                     ATFKNIVITHHAPSLQSVPEMYKDDPVTSAYASHLEDFILEYQPEYWIHGHIHTPVKY
                     EIGKTRIICNPHGY"
     gene            complement(710..>949)
                     /locus_tag="PK198_16705"
     CDS             complement(710..>949)
                     /locus_tag="PK198_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRF40435.1"
                     /translation="ARLKQVFHDFVYVVFGLLDEDATEGNDGTLDGLMQLIIDIRADA
                     RGRKDWGTSDKIRDALKSLRLQLKDGKEGTSWGRE"
     assembly_gap    950..980
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<981..2212)
                     /gene="cysS"
                     /locus_tag="PK198_16710"
     CDS             complement(<981..2212)
                     /gene="cysS"
                     /locus_tag="PK198_16710"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRF40436.1"
                     /translation="MEHNLKLYNSVTREKEAFKPMQDGHVGMYVCGPTVYYDVHLGNC
                     RTFVSFDVIYRYLQYIGYRVRYVRNITDVGHLLDDGEDRMLKGARLEQLEPMEVAQKY
                     TVGFHEMMRIFNALPPSIEPRATGHIPEQIEMVQDILKNGYGYEANGSVYFDTLKFIE
                     KTGIYGGLSGRNVEELVAESRDNLKNQSEKRHPADFAIWMKASPEHIMRWRSPWSEGF
                     PGWHLECSAMSTKYLGPEFDIHGGGNDLKFPHHENEIAQNYGSCQCKGARYWMHTNML
                     LLNGKKMSKSDGNTISPPELFVGDSEHVSKGYSPMVVRFFMLQTHYRSTLDLTDEALQ
                     AAEKGFRRLMDADKTLQSLQPDAAASPGPLDAELNSLMDGVYEEMDDDFNTPKTLARI
                     FELVSRINSLKGGQLPIS"
     gene            complement(2421..>3197)
                     /locus_tag="PK198_16715"
     CDS             complement(2421..>3197)
                     /locus_tag="PK198_16715"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRF40437.1"
                     /translation="IGRQNAIREALAVAFDPNQKFAYTHQIEQLESEITALRAQLPAD
                     AFSTKSSSSAGKTNAYASPAIYFSYAWGDSEESGESRETIVNQLYDALKADGFALKRD
                     KMDLEYKGFISEFMQEIGRSGLVVVAISDKYLRSPYCMHELYEIYRNSRQEKDEFSKR
                     VFPIRVESIKMSDPFIIEEYLAYWEEQETKWKNLVQKRASQLSPAHFNEYDKVREINA
                     KFSELLAFLQDMNSMSKKLLAQDDFAIVKDAVRDRIQELG"
     assembly_gap    3198..3208
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3209..3527)
                     /locus_tag="PK198_16720"
     CDS             complement(<3209..3527)
                     /locus_tag="PK198_16720"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40438.1"
                     /translation="MQDQVRQLIAEDAIEDALKLLETLNSDEVILLKGQYASLQRQNN
                     NGVLKQDEYDIKLNRIKAAALSLAKKLPKDAPVPTPVNNPSPATVPNTSSDQAAGIQR
                     QIDL"
     assembly_gap    3566..3601
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3640..4401)
                     /locus_tag="PK198_16725"
     CDS             complement(3640..4401)
                     /locus_tag="PK198_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF40439.1"
                     /translation="MIRAKNIFKSFDDTPVLHDISTEFLKGTNNLIIGRSGAGKTVLL
                     KLLVGLLQPTSGSIWYDDLDFFSLNKKQLRDLRMQIGMLFQGSALFDSLTVEENVRFP
                     LDMFTNMTPKEKLDRVNYCLDRVSLPGSNKKLPSELSGGMQKRVGIARAIVLQPKYLF
                     CDEPNSGLDPKTALVIDELISDITKESNITTVINTHDMNSVMEIGENIILLYNGRIAW
                     RGSKDDVMHSDNEILHDFIFASPFLKRLRDKAMSV"
     gene            complement(4420..5334)
                     /locus_tag="PK198_16730"
     CDS             complement(4420..5334)
                     /locus_tag="PK198_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF40440.1"
                     /translation="MTELQETPTNALHPAMCGSLLIDKMAQRAATYIHRITNLLRSTK
                     KPVLLALSSVKVMQPLRLFFHIGRYVTMLNNAFSRPEKHIMYYRETIRQMHDIGIGSL
                     PIVFLISIFIGAVAALQFAFQLDGTLVPIYYIGYIVRDFAIIEASPTITALVLAGKVG
                     SNMASEIGGMRQKEHIDAMEIMGVNTAAYLMMPRITAALTVIPMLVALSAFISIFGGY
                     LAVVPTGWMSDPEYVFGVRSFFDPYNFRLMFVKAFTFAFILTTVSCYQGYFVKAGSIQ
                     LGKASTNAVVFSDILILLADYLIAVLLT"
     gene            5422..5685
                     /locus_tag="PK198_16735"
     CDS             5422..5685
                     /locus_tag="PK198_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRF40441.1"
                     /translation="MKPIGDRVVIKPAPAEEKTKGGIIIPDTAKEKPQRGEVVAVGPG
                     KDGNLMTVQVGDIVLYGKYAGQEINFNGEDFMIMREDDVLVIL"
     gene            5732..>6859
                     /gene="groL"
                     /locus_tag="PK198_16740"
     CDS             5732..>6859
                     /gene="groL"
                     /locus_tag="PK198_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRF40442.1"
                     /translation="MAVKEISFDRDAREKLRSGVDALANAVRVTLGPKGRNVVIQKSF
                     GAPQITKDGVTVAKEIELEDAVENMGAQMVKEVASKTADLAGDGTTTATVLAQAMINA
                     GLKNVTAGANPMDLKRGIDKAVKAIIADLKSQSEQIGDDFEKIRQVGAISANNDDEIG
                     SLIADAMKRVSKDGVITVEEAKGTDTYVEEVLGMQFDRGYLSPYFVTNADDMTVEYEN
                     PYILIYDKKIANMQDIVPILEKVIQSGRPLLMISEDVESQALGVLVVNRLRANLKVVA
                     VKAPGFGDRRKAMLEDIAILTGGTVVSDEKGYKLDATTIDMLGQAEKILVDKDNTTIV
                     NGRGHAEDIKARISQIKQQIEATTSDYDREKLQERLAKLSGG"
     assembly_gap    6860..6966
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6967..7392
                     /locus_tag="PK198_16745"
     CDS             <6967..7392
                     /locus_tag="PK198_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRF40443.1"
                     /translation="AVEEGIVAGGGVALVRAIAALDSVQGANEDENVGVQIVRKSLEA
                     PLRIIASNAGLEGSVVLQKVAEGKGSFGYNARTDVYEDMKKAGVIDPTKVTRVALENA
                     ASIAGMVLTTECVVNDKPEKGGGAMPMPPGGMGGMGGMM"
BASE COUNT         1727 a         1828 c         1968 g         1784 t
ORIGIN      
        1 atagccatgc ggattacaga tgatccgggt tttgcctatt tcatatttta ccggcgtatg
       61 gatatgtcca tgaatccagt attccggctg atattccaaa ataaaatcct caaggtgaga
      121 agcataagcc gaagttaccg ggtcatcctt gtacatttcg ggaacggatt gcaaactggg
      181 agcatggtgg gtgattacaa tatttttgaa tgtagcagac tcctccaaac ttgccgccag
      241 ccagaatttt gaatgttgat gaatgtgagc aatatcaaca gatcgcattt tggagtagga
      301 tgggtctctg cgaatccgct tgaaatcgtt cactctcaat tggcaaatgg taccgtaaag
      361 aaacgggttt ccgtacagcg caaaatcggt ccacagcgtt gctccgtgaa acgtgatgcc
      421 ctctatttcg atgcgcttgt tttccaaaac caatacattc gtgtccttcg cggcgtccgt
      481 tatttggtgg agcgttttgg gatacgaacc attgtaatat tcatggtttc caagcaaata
      541 gatgaccggc ttatctggaa tttttgcctt gacccattca accccctttg ttcctacatg
      601 agtatcccct gccaaaatga ctacatcggc ctgctcgaaa ttcgtttccc cttctccgaa
      661 ttccaggtgt aaatcactga taatctgtat tttcataaag agtcaatgcc tactcccggc
      721 cccagctcgt accctccttg ccgtccttga gttgcaggcg gagcgacttg agcgcgtcgc
      781 ggattttgtc ggaagtaccc cagtcttttc gacctctggc gtcggcgcga atgtcaataa
      841 tgagttgcat cagtccgtcc aaagtgccgt cgttgccttc cgtagcatcc tcatccagca
      901 ggccgaaaac gacatataca aaatcgtgaa acacctgctt gagccttgcn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn tctgaaatgg gcaattggcc gcctttgagc gagttgatgc
     1021 ggctcaccaa ttcgaagatg cgggccagcg tcttgggcgt gttgaagtcg tcatccattt
     1081 cttcatacac gccgtccatg aggctgttca gctctgcatc caatggaccg ggacttgcgg
     1141 ctgcgtcggg ctgcaacgac tgcagggttt tgtcggcgtc catcagtcgg cggaagccct
     1201 tttcggcagc ttgcagagct tcatcggtga ggtctaaggt gctgcgatag tgggtttgca
     1261 gcatgaaaaa gcgtaccacc atcgggctgt atcctttgga aacgtgctcg ctgtcgccga
     1321 cgaagagttc gggcggcgag atggtgttgc cgtcgctttt cgacattttt ttgccgttga
     1381 gcagcagcat attggtgtgc atccagtagc gggcgccttt gcattggcag gagccgtagt
     1441 tctgggctat ttcgttttcg tggtgcggaa atttgaggtc gttgccgccg ccatgtatgt
     1501 cgaattccgg ccccagatat ttggtgctca tggccgagca ttcgaggtgc cagccgggaa
     1561 aaccttcgct ccagggcgag cgccagcgca tgatgtgttc gggcgatgcc ttcatccaga
     1621 tggcgaagtc ggcggggtgc cgtttttcgc tttggttttt gaggttgtcg cgcgattcgg
     1681 ccacgagttc ctccacgttg cgcccggaaa ggccgccgta aatgcctgtt ttttcaataa
     1741 atttgagcgt atcgaaatac acggaaccat tggcttcgta gccgtacccg tttttgagta
     1801 tgtcctgcac catttctatc tgttccggta tgtggccggt agcgcgcggc tcgatgctgg
     1861 gcggcagggc gttgaaaatg cgcatcattt cgtggaaacc cacggtgtat ttttgagcca
     1921 cttccatggg ttccaactgc tccagacggg cgcccttgag catacggtct tcgccgtcgt
     1981 ccagcaagtg gcctacatcg gtgatgttgc gaacgtagcg cacgcgatag ccgatgtatt
     2041 gcaagtagcg gtaaatgaca tcgaaactga caaaagtgcg gcagttgccg aggtgaacgt
     2101 cgtaataaac cgtagggccg cacacataca tgcctacatg tccgtcctgc atgggcttga
     2161 aagcctcttt ctctcgtgtg acgctgttgt acagcttcag gttgtgttcc attgttgtcg
     2221 ggaaattatt cggcctcaag ctgcgaactg caagcctcaa gcgacgcgtg ttgtaatcag
     2281 actgcaaaaa tatggaatta gagctatccg cgcaaggcgt tccaaacaaa attttggata
     2341 gcagggagca ttgggtagtg cagggtgact gtcaggttgt aatcttggca gagtccccct
     2401 tcactaagca gtactgggca tcaccccaat tcctgaatgc ggtcccggac ggcgtctttc
     2461 acgatggcga agtcgtcttg ggccaatagt tttttgctca tcgagttcat gtcttgcagg
     2521 aaggccagca gttcgctgaa tttggcatta atttcacgca ccttgtcgta ttcgttgaag
     2581 tgcgcaggac tgagctgcga ggcgcgtttt tgcactaagt ttttccattt cgtttcctgc
     2641 tcctcccaat aagcgaggta ttcttcgatg ataaacgggt cgctcatttt gatgctttcc
     2701 acccgaatgg ggaagacgcg tttggaaaat tcatcctttt cctggcggct gttgcgatag
     2761 atttcataca actcgtgcat acagtacggc gagcggaggt atttatcgct gatggctact
     2821 accaccaagc cgctgcgccc gatttcctgc ataaattcac tgatgaagcc cttgtactct
     2881 aagtccattt tgtcgcgctt gagcgcaaaa ccatcggctt tgagcgcgtc atacaactga
     2941 tttacaatag tttctcggct ttcgccggat tcttctgagt ctccccaagc gtaggaaaaa
     3001 tagatggcag gagaggcgta ggcgttcgtt ttgcctgctg aagaagatga ttttgttgaa
     3061 aatgcatccg ccgggagttg tgcgcgcaaa gcagtaattt cgctttccaa ctgttcgatt
     3121 tggtgggtgt aagcaaattt ctgattggga tcgaaggcta cggccaacgc ttcgcggatg
     3181 gcgttttgcc gcccaatnnn nnnnnnnngg agatcaattt ggcgctgtat gccggcagcc
     3241 tgatcggaag atgtattggg cacggtggca ggtgatgggt tattgacagg tgttggtaca
     3301 ggcgcatctt tgggcagttt ttttgccaaa gacagcgccg ctgcctttat tctgttgagt
     3361 tttatatcgt actcgtcttg cttcaacacg ccgttgttgt tttgcctttg cagggaggca
     3421 tattgacctt tgagcaggat gacttcatcg ctgttcaaag tctccagcag tttgagggca
     3481 tcttcgatgg catcctctgc aatgagttga cgaacctggt cttgcatgac ggaatatttt
     3541 gagtagtttc gagctacgag tttgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nggatttctt tttgcttccc ctcgcagttc caaaaaaaat cacacactca ttgccttgtc
     3661 gcgcaggcgt ttgaggaatg gagaggcaaa gataaaatca tgtagtattt cgttgtcgct
     3721 gtgcattaca tcgtctttgc tgccgcgcca ggcaatgcgc ccgttgtaca gcaaaataat
     3781 gttttcccca atttccatca ccgagttcat gtcgtgggta ttgatgacgg tggtgatgtt
     3841 gctctccttg gtaatgtcgc tgatgagttc gtcaatgacc aaagcggttt tgggatcgag
     3901 gccggagttg ggttcgtcgc aaaacagata cttgggttgc aatacgatgg cccgcgcaat
     3961 gcctacacgt ttttgcatgc cgccgctgag ttcggagggc agttttttgt tggaacccgg
     4021 caggctcacg cggtcgaggc agtaattcac ccggtccaat ttttctttgg gagtcatatt
     4081 ggtaaacatg tccaaaggaa agcgcacatt ttcctccaca gtcagtgaat cgaacaaagc
     4141 cgacccctga aacagcatcc cgatttgcat gcgcaggtcg cgcaactgct ttttgttgag
     4201 gctgaaaaaa tccagatcgt cataccagat gctgcccgac gtaggttgca gcagtcccac
     4261 taagagcttg agcaacacgg ttttaccggc gccgcttcga ccaatgatga ggttattggt
     4321 acccttcaga aattcggtgc tgatgtcgtg cagtaccggc gtgtcgtcaa aagacttgaa
     4381 tatatttttt gcgcgaatca tggttctgag ttttggggat caagtgagca atactgcaat
     4441 gaggtagtcg gccagcaata tgagtatgtc gctgaaaacc acggcattgg tgctggcttt
     4501 gcccaattgg atgctgccgg cttttacaaa atacccctga taacaggata cggtggtgag
     4561 gatgaaggca aaggtgaatg ccttgacaaa catgagccgg aaattgtacg gatcgaaaaa
     4621 cgagcgcacc ccgaaaacgt attcggggtc agacatccag cccgtaggca ctacggccag
     4681 ataaccgccg aaaatactga tgaatgccga cagcgccacc aacatgggaa tgaccgtgag
     4741 cgcggccgtg atgcggggca tcatgaggta ggcggccgta ttgacaccca tgatttccat
     4801 ggcgtcaatg tgttctttct ggcgcatgcc gcctatttcg gaggccatgt tggaacccac
     4861 cttgccggcc agtaccagcg ctgtgatagt gggcgaggct tcgatgatgg caaaatcgcg
     4921 cacgatgtag ccgatatagt atatgggtac caatgtgccg tcaagctgaa aagcaaactg
     4981 gagcgctgct acggcgccga taaaaatgga gatgagaaac acaatcggga gcgagccgat
     5041 gccgatgtcg tgcatttgcc ggatggtttc ccggtaatac ataatgtgct tctccgggcg
     5101 ggagaaagcg ttgttcagca tggtcacata gcgccctatg tggaagaata gtcggagtgg
     5161 ctgcattact ttgacacttg aaagggccaa aagtacaggt ttttttgtgc tccgcaaaag
     5221 attagtgatt ctgtgaatat aggtggcagc ccgttgcgcc attttgtcaa taagcaggct
     5281 gccgcacatc gccgggtgga gggcattggt cggggtttcc tgcaattctg tcatagaaac
     5341 aatatttggc acaaggtgtg caggggggag gatgtccaaa caattatttt atcaccaacg
     5401 ataacaaaat tacaagttat gatgaaacca atcggcgaca gagtggtcat taagcctgct
     5461 ccggctgaag aaaagaccaa aggcggaatt atcattcctg atactgccaa ggaaaaaccg
     5521 cagcgcggcg aagtagtggc tgtaggcccc ggcaaagacg gcaacctgat gaccgttcag
     5581 gtgggcgaca tcgtgctgta tggcaaatat gccggccagg aaatcaactt caacggcgag
     5641 gattttatga tcatgcgcga agacgacgtg ctcgttatcc tctaattttc aatctctttc
     5701 cgttttacaa aaccaaacca acacatccgc tatggctgta aaagaaatca gttttgatcg
     5761 cgacgcccgc gaaaaacttc gctcaggcgt ggatgcgctc gccaatgctg tgcgcgttac
     5821 cctcggcccc aaaggccgca atgtcgtcat ccagaagagc ttcggcgctc ctcaaatcac
     5881 taaagacggc gttaccgttg ccaaagaaat cgaactggaa gacgcagtag aaaatatggg
     5941 cgctcagatg gtcaaagagg tggcttccaa aacggccgac ctcgccggcg acggcaccac
     6001 tacggctacc gtactcgctc aggccatgat caacgccggc ctcaaaaacg tgaccgcagg
     6061 cgccaatccc atggacctca agcgcggcat tgacaaagcc gtgaaggcca tcattgccga
     6121 cctcaaaagc caatccgagc aaatcggcga cgattttgaa aaaatccgcc aggtaggcgc
     6181 tatctctgcc aacaacgacg acgaaatcgg ctcgctcatc gccgacgcca tgaagcgcgt
     6241 gagcaaggac ggcgtcatca cggtagaaga agccaaaggc accgatacct atgtggaaga
     6301 agtgctgggt atgcagttcg accgcggtta cctctctccc tacttcgtga ccaacgccga
     6361 cgacatgacc gtagagtacg aaaatccgta catcctcatc tacgacaaga aaattgccaa
     6421 catgcaggac atcgtgccca tcctggagaa agtcatccag tcgggccgcc cgctcctcat
     6481 gatctctgaa gatgttgaaa gccaggcgct tggcgtactg gtagtcaacc gtctgcgcgc
     6541 caacctcaaa gtagtggccg tgaaagctcc cggcttcggc gaccgccgca aagccatgct
     6601 cgaagacatc gccatcctca ccggcggcac ggtcgtaagc gacgaaaaag gctacaaatt
     6661 ggatgcgacc accattgata tgctcggtca ggccgaaaaa atcttagtgg acaaagacaa
     6721 caccaccatc gtgaacggcc gcggacatgc cgaagacatc aaggcccgca tcagccagat
     6781 caaacagcaa attgaagcca ctacttccga ctacgaccgc gagaaactgc aagagcgcct
     6841 cgccaaattg tcaggcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnngccg tagaagaagg catcgtggcc ggcggcggtg tggctctggt acgcgccatt
     7021 gctgcattgg acagcgttca aggcgccaac gaagacgaaa acgtgggcgt gcagatcgtg
     7081 cgcaaatcgc tcgaagctcc gctgcgcatt atcgcttcca acgcaggcct cgaaggctcg
     7141 gtcgtgcttc agaaagtagc ggagggcaaa ggcagcttcg gctacaatgc ccgcaccgac
     7201 gtgtatgaag acatgaaaaa ggccggcgtc attgacccga ccaaagtgac ccgcgtggcc
     7261 ctcgaaaatg ccgcttccat cgccggtatg gtgctcacta ccgaatgtgt ggtgaacgac
     7321 aagccggaaa aaggcggcgg cgccatgccg atgcctccgg gcggtatggg cggcatgggc
     7381 ggcatgatgt aatgcctgcc cgcaaccgta tgcttagcgc ataatgacgt ataataaagt
     7441 ggaaagccgc tccgaggatt cggggcggct tttgtttttt agattatcaa tt
//
LOCUS       DAORLF010000230         7454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5369_length_7454_cov_2.004325, whole genome shotgun sequence.
ACCESSION   DAORLF010000230 DAORLF010000000
VERSION     DAORLF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5369_length_7454_cov_2.004325"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK198_16750"
     CDS             complement(<1..634)
                     /locus_tag="PK198_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF40444.1"
                     /translation="MEDRFKTLSNGLLLKQTKNADGTRTDYWKMDQPHAPYLFMVAVG
                     EYAVVEDKWRNVPLQYYVEPDYAADAKAIFPYTPEMLEYFSKTFSYDYPWKKYAQIVV
                     RDFVSGAMENTTAVTFGEFVQKHKMDLVDERTNEKIVAHEMMHHWFGDLVTCESWSNL
                     TLNEGFANYSEYLWLENKYGRDEADYHLLEEWQGYFSSADMSIHPLIHFSY"
     gene            complement(675..1286)
                     /locus_tag="PK198_16755"
     CDS             complement(675..1286)
                     /locus_tag="PK198_16755"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40445.1"
                     /translation="MKKFLLLFGILIAFAACNTSKNAVITKVPDNAPEIALDADAEEE
                     AESEDEMADDPGNYVADVYQATNRRAHDLIHTRLDLRFNWAEEKVIGKAELVLRPYFH
                     ASDKVTLDAKGFEFQKVSLSGKNMPLKYTYENDQIIIQLDRSYTRKEQYSILIEYTAM
                     PSASGGSDAITSNKGLFFINPRGEEAGKPMQIWTQELPLVSYH"
     gene            complement(1366..2145)
                     /locus_tag="PK198_16760"
     CDS             complement(1366..2145)
                     /locus_tag="PK198_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF40446.1"
                     /translation="MNQQIQKEGRFKYIESGGSGDNLLLLHGLFGALSNFESIIRHFG
                     ERYNVVVPILPIMELPLRKLSVMGLVEYVADFVEFKGYDGVHVLGNSLGGHVALLYVL
                     QHPYKVQSVILTGSSGLFESAMGTSFPKRGDKEFIRRKTQGTFFRPEVASEELIDEVF
                     DIVNDRNKAIRVIATAKSAVRHNLGDNLGSITAPVLLIWGKDDQITPAFVAEKFHELL
                     PDSRLLFINECGHAPMMEHPELFNQYLEAFLVEVAAAKVPA"
     gene            2445..3200
                     /locus_tag="PK198_16765"
     CDS             2445..3200
                     /locus_tag="PK198_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF40447.1"
                     /translation="MRLIAKLLILTLVFGSLTACVSKKKFDELQASKEAVDRALAETQ
                     TRVKALEGQNAELQSTLESEKTRLNGEIAAIRKDLDATKGQMSQLQSKLSMTEAELAK
                     LRAEIDGIFNTYKNSGLTLENRSGRLYVMTAPVNFGNGSSSLSRDQRKALDELAMTLK
                     NNPNVKVLVEGHTDNKQFAAGSGMDNWQLSVNRSMSVVRYLIRKGVKPAQVAAVGRGE
                     NLPEGDNATKEGRAKNRRSAVAPDVELGGLKQN"
     assembly_gap    3320..3401
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3402..3645)
                     /locus_tag="PK198_16770"
     CDS             complement(<3402..3645)
                     /locus_tag="PK198_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40448.1"
                     /translation="MMIRNILILFLAFSAMQCARVTPIGDLLEAPEDYQKPGSATIKG
                     TVTSKLDFMGLSTFKVMDADGDEIRVVSNQSYKKGDV"
     gene            3715..>4389
                     /locus_tag="PK198_16775"
     CDS             3715..>4389
                     /locus_tag="PK198_16775"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRF40449.1"
                     /translation="MQSLQRTIEAAWNDRSLLADPAVITAVQEVLEMLDAGQVRIAEP
                     DGEGGWRVHEWIKKAVVLYFPLQQMQTIELGPYEYHDKIPLKRNYAALGVRVVPPAVA
                     RYGSFIEKGAILMPSYVNIGAYVGGGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLE
                     PPQAAPTIIEDDCFIGSRCIVVEGARIEREAVLGANVVITQSTRIIDVTGEAPVTYRG
                     RVTAGSV"
     assembly_gap    4390..4549
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4550..6400)
                     /locus_tag="PK198_16780"
     CDS             complement(<4550..6400)
                     /locus_tag="PK198_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRF40450.1"
                     /translation="MNYLTIDNITKTYGEKVLFSGISLQISKGQKVALVAKNGTGKTT
                     LLRVIAGQEAPEGENASVFVHRDVRVGYLSQDPEFYEQSSVLDAVFDSDTELTRTIKQ
                     YEHLLIKPGSDAEMQTILSRMDDLKAWDFEARVREMLTRFNLHNFDQIVATLSGGQRK
                     RLALVRLILSEPEFLILDEPTNHLDLDMIEWLEGYLSQPNFTIFMVTHDRYFLERVCN
                     TIVELDQGQLYKYAGNYSDFLEKKAIREENENIAFDKGRKLLGKELDWMRRQPQARTT
                     KAKSRIDAFYDLQDKMSNTRDNTELQIDIKGQRLGKKVLELHNVGKKFGDKVVLEPFS
                     YKFQRHERIGIVGPNGVGKSSFLRLLTGELRPDSGKVVQGDNTIFGYYSQDGLQLPDD
                     KRVIEVVQDIAEYIPLEKGQKLGAAQLLERFLFSRKQQLVYVSQLSGGEKRRLYLLTV
                     LMQNPNFLILDEPTNDLDVISLNILEEFLMEFPGCILIVSHDRFFLDKLAQHLFIFEG
                     QGRIKDFNGDYTEFREIQKEQEREQRRQERSEQQKIKEAQKPATVGGLTQEERKELQR
                     IEKDIAKLEARRKDIEALFSDQEGLSPQKIEELGRELSSLGSQIETKEMRW"
     gene            complement(6505..>7454)
                     /locus_tag="PK198_16785"
     CDS             complement(6505..>7454)
                     /locus_tag="PK198_16785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40451.1"
                     /translation="NAGSVAWTTCNLPGADAPTDVKAHPTNANIVYMTAGSGVFKSTD
                     KGITWTNISSGLPALPINCIVYDRNSDEGLYIGNQAGVWYRDNSIGGWVHFSNGLPVV
                     DVRELEIYQDVNPANSRLMAATYGRGVWSSDLYVTSVLPVTLSAFGARCEGAAVVLDW
                     ETQTERNNDYFAVERSSDGKVWQTVATVKGAGNATEQQVYQAKDHRPLSGVAYYRLRQ
                     TDFDRQTALSNVVSLSCEGLPAPEVKVYPNPTTGEVQIQGYGQAARLRVYNATGQLVH
                     TLMPADDADALYIGFLPKGVYVFEFSSERMTELHRVVLR"
BASE COUNT         1567 a         1795 c         2066 g         1784 t
ORIGIN      
        1 catagctgaa atggatgagc gggtggatgc tcatatcggc agaactgaaa tagccctgcc
       61 attcttccag caggtggtag tcggcttcgt cgcggccgta tttgttttcc aaccacaggt
      121 attcgctgta attggcaaag ccctcgttga gtgtgagatt ggaccagctt tcgcaagtca
      181 cgaggtcgcc aaaccagtgg tgcatcattt cgtgggccac tattttttca ttggtgcgct
      241 cgtccaccag gtccattttg tgtttttgaa caaactctcc gaaagtcacc gcggtggtgt
      301 tttccatggc gccggagacg aagtcgcgca cgacgatctg tgcgtacttt ttccaggggt
      361 agtcgtagct gaaggttttg gagaaatact ccaacatttc gggcgtgtag gggaaaatgg
      421 ctttggcgtc ggcggcataa tccggctcca cataatattg cagcggcaca ttgcgccatt
      481 tgtcttccac aacggcatat tcgcccacag cgaccataaa tagataaggt gcgtggggct
      541 gatccatttt ccagtagtcg gtgcgggtac cgtcggcgtt tttggtttgt ttcagcaaca
      601 ggccattaga gagggttttg aagcggtctt ccacggtgag ataaatctcc tgcgtgcagc
      661 gctcgttggg gctgtcaatg gtaggaaacc agcgggagtt cctgcgtcca gatttgcatg
      721 ggcttgccgg cctcttcgcc gcggggattg atgaagaaca aacctttgtt ggacgtgatg
      781 gcatcgctgc cgcccgaagc ggaaggcatg gcggtgtatt ctatcaggat gctatactgt
      841 tctttgcggg tatagctgcg atccaactgt atgatgatct ggtcgttttc gtaggtgtat
      901 ttcagcggca tgtttttacc cgaaagggac actttttgaa actcaaaccc tttggcatcc
      961 agcgttacct tgtcggaggc gtggaaatac ggacgcagca ccagttctgc ctttcctatt
     1021 actttttcct ctgcccagtt gaaacgcagg tcgaggcggg tatgaatgag atcatgcgct
     1081 ctgcggttgg tggcctgata cacgtccgcg acataattgc cggggtcgtc ggccatttca
     1141 tcttcggatt ctgcttcctc ctcagcatct gcatcaaggg caatttcagg tgcattatcg
     1201 ggcactttgg ttatgacggc gtttttggag gtattacaag cggcaaaagc gatgagtatg
     1261 ccaaagagca gcaagaattt tttcataaaa aaatattgaa tgtgaaaggg ttggaaaatg
     1321 caaggcagaa cgacggagga ggggcaaatg ttgcaaacgc cgccactaag cgggtacttt
     1381 tgcagcggct acttctacca aaaatgcctc caaatactga ttgaacagtt ccgggtgctc
     1441 catcataggg gcgtggccac attcgttgat gaaaagcagc ctggaatcgg gtagcagctc
     1501 gtgaaatttc tcggctacaa aggcgggcgt tatttggtcg tctttgcccc agatgagcaa
     1561 aacaggcgca gtgatgctgc cgaggttgtc gcccaagttg tgccttacgg cagatttggc
     1621 ggtggcaatg acccgaatgg ctttatttcg gtcgtttaca atatcgaata cttcgtcaat
     1681 gagttcttcg gaggctactt ccgggcggaa aaaagtaccc tgtgtttttc ttctgataaa
     1741 ctctttgtcg ccgcgtttgg gaaaactggt acccatcgcg ctttcaaaca gccctgaact
     1801 gccggtgaga atgaccgatt gcaccttgta gggatgttgc agcacataca gcagggccac
     1861 atggccgccc aaagagttgc ccaatacatg cacaccatcg tagcctttga actctacaaa
     1921 atcggctaca tattccacca accccatgac cgacagtttg cgcagcggca attccatgat
     1981 gggtaagatg ggcactacca cgttgtagcg ctcgccgaaa tggcgaataa tggactcaaa
     2041 attgctcaat gcgccgaaca agccgtgcaa caacagcaga ttgtcgccgc taccgccgct
     2101 ctcgatgtac ttgaaacgcc cctccttttg gatttgctga ttcatgaagg attgatcttc
     2161 agaagtttaa cagtgatgta actcttcaat ggccactgca aaatgctgcg acactcatcg
     2221 tagcttcggt cattgacggt tgcaaaaata gcttttttcg tagtttcggc aaacgcaaga
     2281 tgtaaatctg tgttactaaa atgaaggttt tgccccaaaa gacaaaaaat gctgcgccaa
     2341 actttgtgca atcataaaaa atgccttgct ttgcgtgctg aaaacagccg gtcaaaacaa
     2401 ccggggacga aaacaaaatc attcttcatt aaattgcaaa aaacatgcgt cttattgcca
     2461 agttactcat tctcacgctt gtgttcggct cccttaccgc ttgcgtttcc aagaagaaat
     2521 tcgacgaatt gcaggcttcc aaagaagctg ttgaccgcgc tttggctgaa acccaaactc
     2581 gcgtaaaagc tctggaaggc cagaatgccg aacttcaatc cactctcgaa tccgagaaga
     2641 cccgcctcaa cggcgaaatc gctgccatcc gcaaagacct ggatgctacc aaaggccaaa
     2701 tgagccaact gcaatccaag ctttctatga cggaggctga actcgccaag ctccgcgctg
     2761 aaattgacgg catcttcaac acgtacaaaa acagcggcct gacgctcgaa aaccgcagcg
     2821 gccgtttgta tgtgatgacg gctcccgtga acttcggcaa cggttcttcc agcctcagcc
     2881 gcgaccagcg caaagctctg gacgaattgg ccatgacttt gaagaacaac ccgaacgtga
     2941 aagtgctcgt agagggccac accgacaata agcaatttgc cgccggctcc ggtatggaca
     3001 actggcagtt gagcgtcaac cgctctatga gcgtggtgcg ctacctcatt cgcaaaggcg
     3061 tgaagccggc tcaggtagct gctgtaggcc gtggtgaaaa cctgcccgaa ggcgacaatg
     3121 ccactaagga aggccgcgcg aaaaaccgcc gctctgccgt tgctcccgat gtggaactgg
     3181 gtggtctgaa gcagaactaa ttttgtttgt tagtgaaggg gtgactcgca gtcacccctt
     3241 cactctctta gcttaatagc aaaaaaagcc gtctcgcagc aatgtgagac ggctttttta
     3301 atttatcact ctttggccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctacatctc cttttttgta
     3421 gctctggttg gacaccacgc gaatctcgtc gccatcggca tccatcactt tgaaggtgga
     3481 aagccccatg aaatccagct tcgaggtcac cgtgcctttg atggtggccg agccgggctt
     3541 ttggtagtct tcgggcgctt ccagcaggtc gccgatgggc gtcacgcgag cgcattgcat
     3601 ggcactgaat gccaggaaca ggattaaaat gtttcgaatc atcatatcgg gttttgattg
     3661 gaaactgtgt gtcggcctgg gatgttgcgt acctttgcaa aaaatattac agacatgcaa
     3721 tcactacaac gcacgataga agccgcctgg aacgaccgca gcctgctggc cgatcctgcc
     3781 gtcatcactg ccgtacagga agtattggaa atgctggatg ccggtcaggt ccgcattgcc
     3841 gaacccgatg gagagggtgg ctggcgcgta cacgaatgga tcaaaaaagc ggtggtgctt
     3901 tattttccgc tgcaacaaat gcaaaccatc gaacttggcc cctacgaata ccacgacaaa
     3961 attccactga aacgcaacta cgcggcgctc ggtgtccggg tggtgccgcc ggcagtggcc
     4021 cgctacggca gtttcattga aaaaggcgcc atcctcatgc cctcgtatgt gaacatagga
     4081 gcgtatgtag gcggcggtac gatggtggac acctgggcta cggtaggctc ctgtgcacag
     4141 atagggcgca acgtacacct cagcggaggg gtaggcatcg gcggagtgct ggagccgccg
     4201 caggctgcgc ccaccatcat tgaggacgac tgcttcatag gctcgcgctg cattgtggtg
     4261 gagggcgccc gcatagagcg ggaagcggtg ttgggggcca atgtggtcat cacgcagtcc
     4321 acccgtatca ttgacgtgac gggagaagcg cctgttactt accggggccg cgtgactgcg
     4381 ggttcggtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagcgcattt
     4561 ctttagtttc gatctggctg ccgagcgatg agagttcgcg gcccagttcc tcaatttttt
     4621 gtgggctgag gccctcctga tcggagaaga gtgcttcgat gtctttgcgg cgcgcttcga
     4681 gtttggcaat gtctttttcg atgcgttgca gttcttttcg ctcctcttgt gtgagaccgc
     4741 cgacggtagc cggtttttgg gcctccttga ttttctgctg ttcgctccgt tcctgccggc
     4801 gttgttcgcg ctcctgttct ttctgtattt cgcgaaactc ggtgtaatcg ccgttgaagt
     4861 ctttgatgcg gccctgccct tcgaagatga aaaggtgctg agcgagttta tctaagaaaa
     4921 agcggtcgtg agaaacgatg aggatgcagc ctgggaactc catgaggaat tcttcgagga
     4981 tgttgaggga gatgacgtcg aggtcgttgg tgggttcgtc gaggatgagg aaattggggt
     5041 tttgcatgag cacggtgagc aggtagagcc tgcgtttttc gccgccgctg agttgagaga
     5101 catagaccaa ttgctgtttg cggctgaaga ggaaacgttc gagtaattgt gcggcgccga
     5161 gtttttggcc tttttcgagg gggatgtact ctgcgatgtc ttgtacgacc tcgatgacgc
     5221 gtttgtcgtc ggggagttgg aggccgtctt gcgagtagta gccgaaaatg gtgttgtcgc
     5281 cctgcacgac tttgccggaa tcggggcgca gttcgccggt gaggaggcgg aggaaggagg
     5341 acttgccgac gccgttgggg cctacgatgc cgatgcgctc gtggcgctgg aacttgtagg
     5401 aaaaaggctc cagcacgact ttgtcgccga attttttgcc cacgttgtgc agttccagca
     5461 cttttttgcc gaggcgttgg cctttgatgt caatttgcag ttcggtattg tcgcgggtgt
     5521 tggacatttt gtcctgaaga tcgtaaaaag cgtcaatgcg ggatttggct ttggtggtgc
     5581 gagcctgcgg ctgccggcgc atccagtcca attctttgcc gaggagtttg cggcctttgt
     5641 cgaaggcgat gttttcgttt tcttcgcgga tggccttttt ttccaaaaaa tcggagtagt
     5701 tgccggcgta tttgtagagt tggccctggt cgagttcgac gatggtattg cacacgcgtt
     5761 cgaggaagta gcggtcgtgc gtgaccatga agatggtgaa attgggctga gagaggtagc
     5821 cttcgagcca ttcgatcatg tcgaggtcga ggtggttggt gggttcgtcg aggatgagga
     5881 attccggttc ggagaggatg aggcgcacca aggccaagcg tttgcgctgc ccgccggaga
     5941 gcgtggccac gatttggtcg aagttgtgga gattgaagcg ggtgagcatt tcgcgcacgc
     6001 gggcctcgaa gtcccaggct ttgaggtcgt ccattcgcga gaggatggtc tgcatttctg
     6061 cgtcgctgcc gggctttatc aggaggtgtt cgtattgttt gatggtgcgg gtgagttcgg
     6121 tgtcggagtc gaaaacggca tcaagtacag aactctgctc gtaaaattcg gggtcttgag
     6181 agaggtagcc cacgcgcaca tcgcggtgaa cgaacacgga ggcattttcg ccttcgggcg
     6241 cctcttgtcc ggcaatgacg cgcaggagag tagttttgcc ggtgccgttt ttggccacga
     6301 gggctacttt ctggccttta ctgatttgca gggaaatgcc ggagaaaagg actttttctc
     6361 cgtacgtttt ggtgatgttg tctatcgtca ggtagttcac ggcggcaggt atgcgtattt
     6421 gagggggcga aaatacgcga aagattgggg ggaatgaaga tttatggaaa attcaactgc
     6481 ccggttccca atcataccgc tccatcacct gagtacgacc cggtgcagct ccgtcattct
     6541 ttccgatgaa aactcgaaga catacacgcc tttgggcaag aatccgatgt agagagcgtc
     6601 cgcatcatcg gccggcatta atgtgtgtac caactgtccg gtagcattat agactctcaa
     6661 acgagccgcc tggccatacc cttgaatttg aacctcgccc gttgtcggat tgggatacac
     6721 ttttacctcc ggcgccggca gcccctcgca gctaaggctc accacattgc tgagcgccgt
     6781 ttgtctgtcg aaatccgttt ggcgcagccg gtaataagcg acgccgctca gcgggcggtg
     6841 gtctttggcc tgatacactt gctgctctgt tgcattgccc gcgcctttga cggtggccac
     6901 agtttgccac actttgccgt ccgaagagcg ctctacggca aagtagtcgt tgttgcgctc
     6961 cgtttgcgtt tcccaatcga ggactactgc agccccctcg cacctggcgc cgaaagcgct
     7021 gagcgtaacc ggcagtacgg aggtgacgta caaatcgctg ctccacaccc cccttccgta
     7081 ggtggcagcc atgaggcggc tgttggcggg gttgacatcc tgataaattt cgagttcgcg
     7141 tacatccact acgggcaggc cattgctgaa atgcacccag ccgccgatgc tgttgtcgcg
     7201 gtaccacacg cccgcctgat tgccgatata cagcccctcg tcgctgttgc ggtcatacac
     7261 gatacagttg atgggcaagg ccggcagccc ggaggaaatg ttcgtccagg tgatgccctt
     7321 gtcggtagat ttgaacacgc cgctgcctgc cgtcatatat acgatattgg cattggtagg
     7381 atgcgctttt acgtcggtgg gcgcatcggc gccaggcagg ttgcaggtgg tccaggcaac
     7441 agaaccggca ttgg
//
LOCUS       DAORLF010000231         7431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5386_length_7431_cov_2.091649, whole genome shotgun sequence.
ACCESSION   DAORLF010000231 DAORLF010000000
VERSION     DAORLF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_7431_cov_2.091649"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..>3933)
                     /locus_tag="PK198_16790"
     CDS             complement(63..>3933)
                     /locus_tag="PK198_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40452.1"
                     /translation="TVTNNPTHNGTVEFSVPQPGTYNISVEDGKSCGVSFQMDMSTCT
                     AVTFRLPFTNALPGTTVCLDVRVEDFNSVGAMQFSMQWDPAVLQYSSVQVFNPALLGF
                     DQSAFNVTPPLTSTGRLTVSWADLSFTGVTLPDGASIFQVCFTVIGPLGSFSEINFTD
                     APTDIEIGDAMLNPYGFVGRAGIVDVNNSQLFVNITQDSVSCAGIADGSFTLTVANGT
                     PPYVFNWNSLAPLPVQSGGDAIPNSGGSFTVSGRAAGSYRVVITDSSVPALSRTDTVI
                     VLRGPFIGVNLEPTLPLCNGDSNGSVTARVSLDGVVTPNPPGFSYAWNVPGSPNAPSL
                     SNLPFGFYSVTATAADGCSGTASLTLTQPAPINITETITAAACSGSANGSITANVSGG
                     TPNSGNYTFAWSGGLGTVISSSSTVANLNPGQYSLTVTDANGCSSTETYTVGAVKTLN
                     INAAVTDITCNGLCNGAILVNGTTSGAPAATPYTFTWSGGPAPTNTPTSSNIMGLCAG
                     TYNLTMSDSDPAGCRVTRTFTLAEPAPLVASLIEQVNETCVTGNDGRAIPGVTGGTFP
                     YTYQWRNAAQAVVSGDSIATGLSAGAYTLSVTDGRNCTTTLNVTILAPMPPSIAPIQN
                     DTVSCAGSTDGALSVSATPGSSPIASYVWSNGMNGQNISGLSPGSYTVTVTAQDACFS
                     VATAQVISPAPLVIDSIVAISPTCPGLSNGSLTVFARGGTGPYRYIWANTPQNDTLAF
                     NIYPSLRAGNYTVTVVDANNCPVVSQTATVNDPPSIQINFSNVQGVSCFEGTCNGRAL
                     ATAQYSNGTPGSFNFNWESGLVALGVNSSNATNLCAGFQTLVVSDVNNCFTVDSINIP
                     TPPAIGVFVDAQPVSCNGLSDGTVTLNVNGGTPNYQFLWLETGAVTPTVNNLAAGQYN
                     AVITDANGCIKTQRVEVTQPDRLILSVDLANSTSSVSCAGSTDGVIRVFFNSADNINP
                     VGPNPYSWSSNVAPASSPLASNLSPGMYSVTITDTKGCRDSLNFNITSPPPIVAVIPQ
                     PDEPLCFGQSTLIIIESISGGNGTMLSDYTYMVDNNGLTFSPDQPATVFAGPHIVTIM
                     DAAGCTFEQTLTINQPQELQVVFNPDIVVVELGDSTTRLNPLVTSSLPIATFTWTPTD
                     YLLDSTTQRPLLYLPLEDTEYTLTITDVNGCTASGSVLVEIDKNRNVYIPNAFSPNGD
                     GPNDEFRVFACRGVSDITFVRIFDRWGNFLFEQQSITPDCIGGARLWDGRVKGKPVNE
                     GVYVYMVQVQFLDGVTLLYRGDVTILR"
     assembly_gap    3934..3969
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3970..4725)
                     /locus_tag="PK198_16795"
     CDS             complement(<3970..4725)
                     /locus_tag="PK198_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40453.1"
                     /translation="MIRSFLLFLLLLPAAFAAAQMPRNITVAPGARVESESPARPKSA
                     MMCNNAGTVTFGSIIGQSNDGAPGVIYLCFGDTLPIIHNGDFVLSGDPQPSTPPGIGY
                     AFYDCPPTVHGPTLTDIQGDACLNNTSPIIINGTPVPQSANGMWVASQQINGNAMFAN
                     NGILQSAFNNGIARPIQFWFAPITLDNFATQGFEASGGAAGPCVNVNTAAAFSVVYLN
                     AIQAANFNTTAGVNGCEGSFTIRGGLPEFDPNTS"
     assembly_gap    4791..4842
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4957..5583)
                     /gene="pyrE"
                     /locus_tag="PK198_16800"
     CDS             complement(4957..5583)
                     /gene="pyrE"
                     /locus_tag="PK198_16800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRF40454.1"
                     /translation="MSIASELANKLLQIKAIRLSPQNPFTWASGWKSPIYCDNRIILS
                     HPALRRFVVQGFVELARSMPPFDAVAGVATAGIAHGVLLAEALDLPSVYVRAKAKEHG
                     RRNMIEGELQPGSRVLVVEDLISTGGSCLAAVEALRDAGAEVAGVTAVFSYGFAEADA
                     AFAAAGCPWATLSHYNVLIEAAQELGVVSAADMEMLRDWRSRPTEWGR"
     gene            5617..6243
                     /locus_tag="PK198_16805"
     CDS             5617..6243
                     /locus_tag="PK198_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF40455.1"
                     /translation="MYKVYINQTPLLLSTEPLAEVAADKEHCLSARYTGRPKTLLNYI
                     DMMEKGRRFRSVNLWFPDVEQLFSDFCAHHRIIEAAGGIVYNSDKKLLLIHRLGYWDM
                     PKGKIDAGETPEEAALREVREETGVVELYSDGFALTTYHTYRDRDERRALKPSHWFYM
                     HTPETKLTPQTEENIEKAVWTSPEHFLELGWEAYESIRDILQIEFNKI"
     gene            6416..6778
                     /locus_tag="PK198_16810"
     CDS             6416..6778
                     /locus_tag="PK198_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40456.1"
                     /translation="MLKFVMAVVLLISGATFLGAQSLSHTTGSDAKTAQVNQDDKVIF
                     ADQTNKTFFIDFERINVNLSGIVVKDILGGVVFKDEVANLPVDTIYELNLSRMSPGTY
                     DIELQSYTGVIKKTVTLP"
     gene            6839..>7431
                     /locus_tag="PK198_16815"
     CDS             6839..>7431
                     /locus_tag="PK198_16815"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRF40457.1"
                     /translation="MAYPSLESAALDLEKHGHLIRISEEVDPNLEVAEIHRRIFDAGG
                     PALLFERVKGSPFRALSNLYGTFERTEFLFRHTLDKVAKVIELKADPAALLRKPTRYL
                     SAPVTALSALPMRVRSGPVAYGTTTIDQLPLVKSWPMDGGAFVTLPQVFTLPPGDNNI
                     MHSNLGMYRIQLTGNEYITNKEVGLHYQLHRGIGVHHT"
BASE COUNT         1757 a         1959 c         2006 g         1621 t
ORIGIN      
        1 aaatgaaacg tttacttatc taatcctttg gcgaaaatga ccactttatt ttttttcgcc
       61 ccttatcgga gtatcgttac atctccgcga tacaacaagg tgacgccatc gaggaattgc
      121 acctgtacca tatatacata cacgccctca ttgaccggct tgccttttac acgaccgtcc
      181 cagaggcggg cgccgccgat gcagtcgggc gtaatgctct gttgttcaaa gaggaaattg
      241 ccccagcgat cgaaaatgcg aacgaaagta atatccgaaa cgccccggca ggcaaacacc
      301 ctgaactcat cgttggggcc gtcgccattg ggagagaatg cgtttggaat atagacgttg
      361 cggtttttgt caatttccac caatactgag ccggaagcag tacaaccatt cacatcggtg
      421 atggtgagcg tatattcggt gtcctctaag ggcagataca acagtggtcg ctgtgtcgtg
      481 gagtccaaca gataatccgt cggcgtccag gtaaaggtag caattggaag cgaagaagtg
      541 accaaaggat tgaggcgggt ggtggaatcg cccagctcta ccactacaat gtccgggttg
      601 aataccacct gcaattcctg cggctgattg atggtcagtg tttgttcaaa cgtacatcct
      661 gccgcgtcca tgatcgtgac aatatgcggg ccggcaaaaa cagtggccgg ctgatcgggc
      721 gagaaagtca agccgttgtt gtccaccatg taagtataat ccgacaacat agtgccgttg
      781 ccgccggaaa tactctcaat aataatgagt gtggactgtc caaagcagag cggttcgtcg
      841 ggttgcggta tcacagctac aatgggcggc ggcgaggtga tgttgaaatt caggctgtcg
      901 cggcagcctt tggtatcggt gatggtcacg gagtacatgc ccggactcag gttggaagct
      961 aaaggtgaag atgcgggcgc tacattactc gaccaggaat acggattcgg cccgaccgga
     1021 ttgatgttgt cggcactgtt gaagaacacc cgaatgacgc cgtcggtact gcccgcgcaa
     1081 gacaccgaag aggtagagtt ggccagatca accgacaaaa tgagccggtc cggctgcgtc
     1141 acttccacgc gctgggtttt gatacaacca ttggcgtctg taattacggc attgtactgc
     1201 cccgcggcga ggttgttgac ggtgggtgtt accgcgccgg tttccagcca gaggaactgg
     1261 tagttgggcg tgcctccatt cacattgagc gtcactgtac catcactcaa cccgttgcaa
     1321 cttaccggtt gggcatctac aaaaactccg atggcgggcg gtgtgggaat attaatagaa
     1381 tctaccgtga agcaattgtt gacatccgaa accactaatg tctggaagcc tgcacagaga
     1441 ttggttgcat tggaagaatt gacgcccaga gccaccaagc cggattccca gttgaaattg
     1501 aatgagcctg gcgttccgtt gctgtattga gccgtagcca aagcccggcc gttgcaggtt
     1561 ccttcaaagc agcttacccc ctgaacattg ctgaaattga tctgaatact cggcgggtcg
     1621 ttcaccgttg ctgtttggct caccacaggg cagttgttgg catccaccac cgttacggtg
     1681 tagttgccgg ctctcagtga tggatatata ttgaatgcca gcgtgtcatt ttgaggcgta
     1741 ttggcccaaa tatagcgata cggcccggtg ccacctctgg caaataccgt gaggctgccg
     1801 ttgctgagac cgggacaagt gggcgatatg gccacgatgc tgtctatgac caaaggcgcc
     1861 ggcgaaatga cctgtgcagt agccacgctg aagcaggcat cctgggccgt caccgtcacg
     1921 gtgtaagacc ccggactgag tccgctgatg ttttggccgt tcatgccgtt gctccacaca
     1981 taactcgcaa tgggcgagct gccgggggta gccgaaacgc tcagcgcgcc gtcggtgctg
     2041 cctgcacaag atacggtgtc gttctgtatg ggcgcgatgc tcggaggcat gggcgccaga
     2101 atagttacat tgagcgtagt agtacagttg cggccgtcgg tcacagagag ggtataggct
     2161 cctgccgaaa gaccggtagc gatggagtcg ccggaaacga ccgcctgcgc agcgtttctc
     2221 cattgataag tatagggaaa agtgccgccg gtgacgccgg gtatggcgcg gccgtcgttg
     2281 cccgtcacgc aggtttcgtt tacctgttct atcaaagaag ccactaaggg agccggttcc
     2341 gccaatgtaa aagtgcgcgt cacccgacag ccggccggat cagaatcgct catggtgagg
     2401 ttgtaagtgc cggcacaaag ccccattata ttgctcgatg tgggcgtatt ggtgggcgca
     2461 gggccgccgc tccaggtgaa ggtataagga gtagccgcgg gcgcgcctga agtggtaccg
     2521 tttaccaaaa tagctccgtt gcacaagcca ttgcaggtga tgtcggtcac agccgcattg
     2581 atattaagcg ttttaacggc gcccacggtg taagtttctg tggaagaaca tccattagcg
     2641 tccgtaacgg taagagaata ttgccccgga ttgagattgg ccaccgtaga actggaagaa
     2701 atcaccgtgc cgaggccgcc gctccaggca aacgtatagt tgccgctgtt gggcgtgccg
     2761 ccgctcacgt ttgccgtgat ggaaccatta gcagaaccac tgcacgccgc agcggtgatg
     2821 gtttcggtaa tattgatagg ggccggttgt gtgagcgtaa gagaagcagt gccgctgcat
     2881 ccatcggctg cggtagcagt aacagaatag aacccaaaag gcaggttgga caacgagggc
     2941 gcattgggcg agccaggtac gttccaggca taagagaatc ctggcggatt gggcgtcacc
     3001 acgccatcca agctgacacg cgccgttacc gaaccattgc tgtctccatt acataatggc
     3061 agcgtgggtt ccaggttcac gcctataaac ggccctctca atacgattac ggtatccgtt
     3121 cggctgagtg ccggcacgct ggagtctgta atgaccacgc ggtacgaacc tgccgcccgg
     3181 ccactcacgg taaaggagcc gccggagttg ggaatggcat caccgcccga ctgtacaggc
     3241 agcggcgcca aactgttcca gttgaacaca tacggcggcg tgccgttggc tacggtcagc
     3301 gtaaacgaac cgtctgcaat gcctgcgcag gaaacgctgt cttgtgtgat gttcacaaac
     3361 aactggctgt tgttgacatc cacaatgcct gcccgcccca caaaaccata cgggttgagc
     3421 atcgcatcgc caatttctat atcggtgggc gcatcggtaa agttgatttc ggaaaaactg
     3481 cccaatggcc cgatgacggt aaaacaaacc tgaaaaatac tcgctccgtc gggcagggtt
     3541 acacctgtga aactcaggtc ggcccatgaa accgtcaggc gtccggtgga ggtcagcggc
     3601 ggcgtcacat tgaaggccga ctgatcgaag ccgagcagcg ccgggttgaa gacttgcacg
     3661 ctggaatatt gcaacacggc ggggtcccac tgcatcgaaa attgcatggc gcccacactg
     3721 ttgaagtctt ctacccgcac atccaagcac acggtagtgc ccggcagtgc gttggtaaat
     3781 ggcagtctga aggtaacagc cgtgcaagtg ctcatgtcca tttgaaaaga aacgccgcag
     3841 cttttgccgt cttccaccga gatgttgtaa gtgccgggtt gcggtactga aaattctacc
     3901 gtgccgttgt gggtcgggtt gttggtcaca gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnna gaggtattgg gatcaaactc aggcaagccg ccgcgaatgg taaacgagcc
     4021 ttcgcagccg ttgacccccg ccgtagtatt gaaattggca gcctgtatgg cattgagata
     4081 tactaccgaa aaggccgcgg ccgtgttgac gttcacacaa ggtccggcag cgccgccgga
     4141 tgcttcgaag ccttgagtgg caaaattatc aagcgtaata ggcgcaaacc aaaactgtat
     4201 gggccgcgct atgccgttgt tgaaggccga ctgaagaatg ccgttgttgg caaacatggc
     4261 attgccgttg atctgttggg aggccaccca catcccgttg gccgattgcg gcaccggcgt
     4321 tccgttgatg atgatcgggc tggtgttgtt caaacaggca tcgccctgga tgtccgtcag
     4381 cgtagggcca tgtaccgtag gcgggcaatc gtaaaaagca tagccgatgc cgggaggtgt
     4441 ggatggctgc gggtcgccgc tgagcacaaa atcgccgttg tgaataatgg gcagcgtatc
     4501 gccgaaacag agatagatga cgccgggagc gccgtcgttg ctctggccga tgatgctgcc
     4561 gaaggtgacg gtgcctgcat tgttgcacat catggccgat ttggggcggg caggcgattc
     4621 gctttctacg cgtgcgccgg gcgctacggt gatgttgcgc ggcatctggg cagcggcaaa
     4681 tgctgcgggc aagagcaaaa gaaacaggag aaatgatctg atcatgagat atccttatcg
     4741 tgtttgtaaa atcggtattg ggaaaacgaa cgttatgaaa cgcctcaaag nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatttgtt tcctgccccg
     4861 ccgcttgcag cttgcggccc acagcctgtc gccgctccta cccgccaaga gccagcagct
     4921 aacagccagc agccaaccgc cgttcctccc cgtcctctac ctcccccact ccgtcggccg
     4981 gcttcgccag tctctgagca tttccatatc agccgccgaa acaacgccga gttcctgcgc
     5041 agcctcaatc agcacattgt aatgactgag ggtagcccag gggcagcctg ccgcagcaaa
     5101 agccgcatct gcttccgcaa atccgtaaga aaacacagcc gttacgccgg caacctccgc
     5161 ccctgcatcg cgcaaagctt ctaccgcagc aaggcaactc ccgcccgtag aaatcaggtc
     5221 ttccaccacc aatacccggc taccgggctg caattcgccc tctatcatgt tgcgccggcc
     5281 atgctctttg gccttggccc gcacatacac ggagggaagg tccaaagctt cggccaacaa
     5341 gacgccgtgc gcaatccctg ccgttgctac gcctgccacc gcatcgaatg gcggcatgga
     5401 gcgagccagt tccacaaatc cctgaacgac aaagcgccgc agggcaggat gcgagaggat
     5461 gatgcggttg tcgcaataaa tcggcgattt ccaaccggaa gcccaggtaa aagggttttg
     5521 cgggctgagc cgaattgctt ttatttgcag cagtttgttc gccagttcgg atgcaataga
     5581 cataaagctg gattatttga gaaataacaa gtgcaaatgt ataaagtcta tatcaatcag
     5641 acgccgttgc tgctcagtac agaacccctt gccgaggttg cagcagataa agagcattgt
     5701 ctttcggccc gctacaccgg ccgccccaaa accctgctca actatataga catgatggaa
     5761 aaaggccgcc gattcaggtc ggtcaatctc tggtttccgg atgtggagca gttgtttagc
     5821 gacttctgcg cgcaccatcg catcattgag gcagcaggag gcattgttta caactccgac
     5881 aaaaagttgt tgctcatcca taggcttggc tactgggata tgcccaaagg caaaattgat
     5941 gccggtgaaa cacccgaaga ggctgcgctc cgggaagtac gcgaagaaac aggcgtggta
     6001 gaattgtact ccgatggttt tgcactcacc acttatcata cctatcgaga ccgcgacgag
     6061 cgacgggcgc tcaagccttc gcactggttt tacatgcaca cgcccgaaac caaactgacg
     6121 ccgcaaaccg aagaaaatat cgaaaaagcc gtctggacaa gtcccgaaca ctttttggag
     6181 cttgggtggg aggcttatga aagtattcgg gatatcttac aaatagaatt caacaaaatt
     6241 taaatcaaaa attaacctct atttatttta ttttaaaaat aaaatttcaa acaggtagtt
     6301 tgtttgatta ttaatttttt tcgacgaaag tattggcaaa atctttacaa acactcaggc
     6361 ttgccgtagc tttgcacccg gtttcatgat aacatatcca aacacaaccg acaacatgct
     6421 gaagtttgta atggcagtag tcctgctgat ttccggcgct acttttctcg gtgcgcaatc
     6481 tctgtctcac actaccggct ccgacgccaa aaccgctcag gtcaatcagg atgacaaagt
     6541 tattttcgcc gaccagacga ataaaacttt cttcattgac tttgagcgca tcaacgtcaa
     6601 tttgagcggc atcgtcgtca aagacattct gggcggtgtg gtgttcaaag atgaagtggc
     6661 caatttgccc gttgacacca tctacgagct gaatttgagc cgcatgtcgc ctggcactta
     6721 cgacatcgaa ctacaatcgt acaccggagt catcaaaaaa acggtgacat tgccctgagc
     6781 cggggcggtt ttcccaaacc attgtttccg gtagctgtta cttttgcaaa aaactgtaat
     6841 ggcatatccg agccttgaat ctgccgcgct tgatctggag aagcacggcc atcttattcg
     6901 catctccgaa gaagttgatc ccaatcttga agtggccgag atccatcgcc gcattttcga
     6961 cgcgggcggc ccggccctgc ttttcgagcg cgtcaaaggc agtccgttca gggccttgtc
     7021 caatctctat ggtacttttg agcgcaccga gtttctcttc cgccacacat tagacaaggt
     7081 tgccaaagtc atagaactca aagccgaccc cgccgctttg ttgcgaaagc ctacgcgtta
     7141 tctctccgct cctgttacgg cgctctctgc actgcccatg cgcgtgcgtt ccggccccgt
     7201 agcttatgga actaccacca tagaccaatt gcctttggta aaatcatggc cgatggacgg
     7261 aggcgccttc gtcaccttgc cacaggtatt tacattgccg cccggcgaca acaacatcat
     7321 gcactccaac ctgggcatgt accgcattca actgacgggc aacgagtaca tcaccaataa
     7381 ggaggtgggg ctgcactacc agttgcaccg gggaataggc gttcaccata c
//
LOCUS       DAORLF010000232         7428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5389_length_7428_cov_2.054116, whole genome shotgun sequence.
ACCESSION   DAORLF010000232 DAORLF010000000
VERSION     DAORLF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_7428_cov_2.054116"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1531
                     /locus_tag="PK198_16820"
     CDS             215..1531
                     /locus_tag="PK198_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRF40458.1"
                     /translation="MIYQPAIETMPVAQLRALQSERFVRLVRYMYEHQPFYRRQWDAH
                     RIDIRSVKSIDDIGRFPFTIKTHLRDEYPFGLMAVPPADLVRIHASSGTTGKPTVVGY
                     TRADIDMFAEVVARSLCCAGALPGMKLHNAYGYGLFTGGLGLHYGGERLGMAVIPVSG
                     GMTDRQLMLLQDFGPEVISCTPSYALTLAEAIRERGIPLEKFKLRYAILGAEPWTETT
                     RQAVEAGLGVTATNIYGLSEIVGPGVSQEDFEEKGGSHIWEDHFFPEIVHPDTGEPQP
                     YNTEGILVFTTLTKQGIPLMRYRTNDICSLYYDSGSKRTHIKMSPIKGRSDDMLIIRG
                     VNLFHTQVEAVLHDMPEFSANYQLIIYREGALDAVEVLVELGEVIYAAIGARLPINDE
                     FLNQLKRRLEKKIKDNIGLSMRITLADRDALPRSEGGKLKRILDRR"
     gene            complement(1458..1784)
                     /locus_tag="PK198_16825"
     CDS             complement(1458..1784)
                     /locus_tag="PK198_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40459.1"
                     /translation="MTSNLEARKLSVIDYLADLNDEALLSQIENLIKPQADFWDEMTE
                     NQRASVLRGLNQVENGEHIAFQDFMNRFKTDRKIDFSKSTPIACPKSFSACHPRCGAE
                     RRGRPM"
     gene            complement(1869..2204)
                     /locus_tag="PK198_16830"
     CDS             complement(1869..2204)
                     /locus_tag="PK198_16830"
                     /inference="COORDINATES: protein motif:HMM:NF020444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="element excision factor XisI family protein"
                     /protein_id="HRF40460.1"
                     /translation="MAKIINIQQLLLSELNMRASRRAANMPMVKRQLIVNKEANHFVL
                     MAFGWAQSRYVHQCLIHLELKDNKIWIHEDLTDPGIFDVLLENGVPARDIVLGYVSEI
                     ERGEQVGVI"
     gene            complement(2192..2605)
                     /locus_tag="PK198_16835"
     CDS             complement(2192..2605)
                     /locus_tag="PK198_16835"
                     /inference="COORDINATES: protein motif:HMM:NF020393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="element excision factor XisH family protein"
                     /protein_id="HRF40461.1"
                     /translation="MAKDIIHKAVRFALEKDRWVITDDPFTLQTGDISVDIDLAAEKF
                     LVAERGIEKILVEVKSFGNRSLIYDFHTALGQYIDYRGALRDEDIEIDLFLAISEETF
                     RTFERTAFFLRRIRENNVKLLVVNLLTESIVLWQK"
     gene            complement(2661..4469)
                     /locus_tag="PK198_16840"
     CDS             complement(2661..4469)
                     /locus_tag="PK198_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF40462.1"
                     /translation="MRSFWRLLGYLRNYRSQVVLNIVSNLLTALFTAIAIPLLQPFLE
                     LLFDRKALITEAPAAGFSFKNISDYFGYTVSRLIVEQGKEAALVYVCIWIVGVFFLKN
                     LFRYLSLFFMAPVRNGIIRDLRGQLFAKLLVLPLSYFSEERKGDLMSRITADVQEVEW
                     SILNVLEAVFREPLIITGALGIMLYISPQLTMFVFALMLFTGFVIGGIGRRLKRSSLD
                     VQERLGSLVSIIEEALGGLRIIKGFNAEGYQRNKFGRENEAYRHTLTRLLWRRDLSSP
                     LSEFLGIGVVAVLLWFGSRLVFSGQMGAETFLTFIFAFYNVIEPAKKLSRAVYDIQKG
                     IAAMQRVEVVLDAPLGIQEAAQPQPPATFQTAIEYRHVGFAYNDTEGPVLQDVNLFIP
                     KGRVVALVGASGAGKSTIADLLPRFYEVQSGGIFIDGVDIRQMSLKNLRSLMGIVSQE
                     AILFNDTIYNNIVFGMEGATPEDVERAARIAHAHEFIMATENGYQTNIGDRGLKLSGG
                     QRQRLTIARAILKNPAILILDEATSALDTESEKLVQQALEELMKNRTAIVIAHRLSTI
                     RHADEIIVMHRGRIVQRGTHEELSRLEGEYRKLTGG"
     gene            complement(4469..5809)
                     /gene="accC"
                     /locus_tag="PK198_16845"
     CDS             complement(4469..5809)
                     /gene="accC"
                     /locus_tag="PK198_16845"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRF40463.1"
                     /translation="MFNKILIANRGEIALRIIRTCREMGIKTVAVYSTADRESLHVRF
                     ADEAVCIGPPASSESYLSIPRIMAAVEITNADAIHPGYGFLAENAEFAEVCSEYGIKF
                     IGPSADQIRKMGDKITAKETMVKAGVPVVPGSDGLVKDLKHGHKIAKDIGYPVIIKAT
                     AGGGGKGMRVVWEEKDFETAYTLARTEAKAAFGNDGVYVEKFVEEPRHIEFQIAGDQF
                     GTVVHLSERDCSIQRRHQKLVEESPSPFMTDELREKMGEAAVKAGQCINYEGVGTVEF
                     LVDKYRNFYFMEMNTRIQVEHPVTEEVIDHDLIKEQIKIAAGERISGKNYYPTMYAME
                     CRINAEDPFHNFRPSPGRISSFHSSKGHGVRVDTHVYAGYTIPPFYDSMIAKLICRAR
                     TREEVIRKMERALDEFIIEGVKTTVPFHQRLMKDENFRNGNFHTGFLNDFDLTQ"
     gene            complement(5864..>6141)
                     /gene="accB"
                     /locus_tag="PK198_16850"
     CDS             complement(5864..>6141)
                     /gene="accB"
                     /locus_tag="PK198_16850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRF40464.1"
                     /translation="SAPSADKGSGAYLEVRSPIVGTFYRSPSPDKPAYVKPGDVIKPG
                     DVVCIIEAMKLFNEIESEHGGTVVKIMVEDASPVEFDQVLFLIDPKG"
     assembly_gap    6142..6151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6152..6365)
                     /locus_tag="PK198_16855"
     CDS             complement(<6152..6365)
                     /locus_tag="PK198_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HRF40465.1"
                     /translation="MNNFKEIQELLRLVSKLELSEFKMKDGEFEMSVRTRHFDRGGHA
                     VAPVMVSAPAVAPAPAPAPVQESTSAP"
     gene            complement(6371..6937)
                     /gene="efp"
                     /locus_tag="PK198_16860"
     CDS             complement(6371..6937)
                     /gene="efp"
                     /locus_tag="PK198_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRF40466.1"
                     /translation="MASTADIRNGLCIEYNNDIFTIVEFQHVKPGKGAAFVRTRMKSV
                     TTGRVLENTFPSGHKIDVVRVERRKFQFLYPDDAGYHFMDNDTFEQTSIMEDMISRPE
                     LLKEGIEVEILYHAEKDIPLNVELPSNVVLQVTYTEPGVRGDTATNTLKPAKVETGAD
                     VKVPLFVNEGDFIRIDTSNGAYMERVKQ"
     gene            complement(7004..>7428)
                     /locus_tag="PK198_16865"
     CDS             complement(7004..>7428)
                     /locus_tag="PK198_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRF40467.1"
                     /translation="GQQYLYQKAGGSRWPASQHTLDNREHFAYQNGKPVFKYAVNGMA
                     DVVRQVMERNQLGQDDVAWVVPHQANLRIIESVANQLNFPIEKVMINIERYGNTTAAT
                     IPLCLWEWENQLKKGDNLVLTAFGGGFTWGAVYLKWAY"
BASE COUNT         1716 a         2053 c         1896 g         1753 t
ORIGIN      
        1 gggatagagc tgttagcttt agcacggcaa tagcccgata aagctaacag ctctatcccg
       61 accgccgcca ttccgtttct atccctggcg cgacgcgaca accaccccgt agggggcaac
      121 ccttgtggtt gcccccaatg ggttgcccca acggttgccc ctaacatgac ttttatcatc
      181 atcggcgcat cgccgacgcc ctaacttagc ccccatgatt taccaacctg ccatcgaaac
      241 catgcccgtg gcgcaactcc gcgccctgca aagcgaacgc ttcgtccgcc tcgtgcgcta
      301 tatgtacgaa caccagccat tctaccgccg ccaatgggat gcccaccgca ttgacattcg
      361 ctccgtcaaa agcattgatg acatcgggcg cttccccttt accatcaaaa cgcatctccg
      421 cgacgagtac cccttcggtc tcatggccgt gccgcctgcc gatctcgtgc gcatacacgc
      481 cagcagcggc accaccggca aacccaccgt cgtgggctac acccgcgccg atattgacat
      541 gttcgccgaa gtggtagccc gctctttgtg ctgtgccggc gccttgcccg gcatgaaact
      601 ccacaatgcc tacggctacg gcctcttcac cggcggactg gggctgcact acggcggcga
      661 gcgcctcggc atggccgtca tccccgtatc gggcggcatg accgaccggc aactcatgct
      721 tttgcaggat tttgggccgg aggtcatttc ctgcacgccc tcctacgccc tcacgctggc
      781 cgaggccatc cgcgaacgcg gcatcccgct cgaaaaattc aagctgcgct acgccatcct
      841 cggcgccgaa ccctggaccg aaaccacccg gcaggcagtg gaggcaggat tgggcgtcac
      901 ggccaccaat atttacggcc tcagcgagat tgtaggcccc ggcgtgtcac aggaggattt
      961 tgaagaaaaa ggcggcagcc acatctggga ggatcatttc ttccccgaaa tcgtacatcc
     1021 cgataccggc gagccgcaac catacaacac agagggcatt ctggttttta ccacgcttac
     1081 aaagcaaggc attccgctga tgcgctaccg caccaacgac atttgcagcc tgtactacga
     1141 ttccggcagc aagcgcaccc acatcaagat gtcgcccatc aaaggccgct ccgacgatat
     1201 gctcatcata cgcggggtca atctgtttca cacacaggta gaggccgttt tgcacgatat
     1261 gccagagttc agcgccaatt accagctcat tatttaccgc gaaggcgcct tagatgcagt
     1321 ggaagtattg gtagaattgg gcgaagtcat atatgcggcc ataggcgccc gcctgcccat
     1381 caacgatgag tttttgaacc agttgaaacg gcgtttggag aaaaaaatca aagacaacat
     1441 cggactgtcc atgcgcatca cattggccga ccgcgacgct ctgccccgca gcgagggtgg
     1501 caagctgaaa aggattttgg acaggcgata ggggtactct tggagaaatc aatttttcga
     1561 tcagtcttga atctgttcat aaaatcctga aaggcaatat gctcaccatt ctcaacctga
     1621 ttcagtcctc tcaaaacaga ggccctttga ttttcagtca tctcgtccca aaaatcagct
     1681 tgtggcttta tcagattttc aatctgactc agcaaggctt catcgttaag atcggccagg
     1741 taatcaataa cacttagttt tctggcttca agattggaag tcatcggcat gtaatttaat
     1801 ctaaaataaa cgcttcaaaa aacaactcgg cttgcatacc aattccagtc atcggcaggg
     1861 cattacactc atatcactcc tacttgctca cccctttcta tttccgaaac atatcccaaa
     1921 acaatatcgc gggcaggcac tccattttcc agcaagacgt cgaagatacc cggatcggtc
     1981 aaatcttcat gtatccagat tttattatct ttcaactcca gatgtatcag gcattgatgc
     2041 acatatcggc tttgcgccca gccaaatgcc atcagcacaa agtggttggc ttctttgtta
     2101 acaatcaatt gccttttcac catgggcata ttggctgcac ggcgcgacgc tctcatattc
     2161 agctcgctca atagaagctg ttggatgttg attatttttg ccatagcacg atactttctg
     2221 taagtaaatt cacaaccaaa agtttaacgt tgttttcccg gattcggcgt aaaaaaaatg
     2281 cagttctttc aaaggttctg aaagtttcct ctgaaattgc cagaaacaag tcaatttcaa
     2341 tgtcctcatc ccgcaatgct cctctatagt caatgtattg ccccagtgct gtatgaaaat
     2401 catagatcag agagcggttc ccgaatgatt ttacctcaac aagtatcttt tcgatacctc
     2461 tttcagctac cagaaatttt tcggctgcta aatctatatc cacagaaata tcacctgttt
     2521 gcagtgtaaa tgggtcgtcc gttatgaccc aacgatcctt ttctaaagca aatctgacag
     2581 ccttgtgaat aatatcttta gccatgttct ttctgttgag gtgaagattt tgcaaatata
     2641 aaacattctg accatacccc tcagcccccc gtcaacttcc tgtactcccc ctccaacctc
     2701 gacaactcct cgtgcgtgcc gcgctgcacg atgcggcccc ggtgcatcac gatgatctcg
     2761 tcggcatggc ggatggtgct cagccggtgc gctatgacaa tggccgtgcg gtttttcatc
     2821 agttcctcca aagcctgctg caccaatttt tccgactccg tatccaaggc cgaagtagcc
     2881 tcgtcgagga tgaggatggc agggtttttc aggatggcgc gggcaatggt gagacgctgg
     2941 cgttggccgc cgctgagctt gaggccccgg tcgccgatat tggtctggta gccgttttcg
     3001 gtggccatga tgaactcgtg cgcatgtgca atgcgggcgg cgcgctccac atcttcaggc
     3061 gttgcgcctt ccataccgaa cacgatgttg ttgtaaatgg tatcattgaa caggatggcc
     3121 tcctgcgata caatgcccat aaggctgcgc agatttttga gactcatctg ccgaatgtcc
     3181 acgccatcaa taaaaatacc tcccgactgc acctcgtaaa accggggcag caaatcggct
     3241 atggtggact tgcccgctcc cgatgcgccc accaaagcca ctacgcgccc tttggggata
     3301 aagagattaa catcctgtaa cacaggccct tcggtgtcgt tgtaggcaaa acccacatgg
     3361 cggtattcga tggcggtttg gaatgttgcg ggaggctggg gctgcgctgc ctcctgaatg
     3421 ccgagcggcg cgtccagcac cacttccact cgttgcatcg cggcgatgcc tttttgaata
     3481 tcataaacgg ctcgggacag ctttttggcc ggctcgatga cgttgtaaaa tgcgaagatg
     3541 aaggtcagga aagtctcggc gcccatttgc cccgaaaaaa cgagccgcga accaaaccac
     3601 agcagcaccg ccaccacccc gatgccaaga aattccgaca agggcgagga cagatcgcgg
     3661 cgccacagca ggcgggtgag cgtgtggcgg taggcttcgt tttcgcgccc gaatttgttg
     3721 cgctgatacc cctcggcgtt gaatcccttg atgatgcgca ggccgcccag cgcttcttcg
     3781 atgatggaca ccaaagagcc gagccgctcc tgcacatcga gcgaactgcg tttgagccgc
     3841 cgcccgatac cgccgatcac aaaaccggtg aacagcatga gcgcaaacac gaacatggtg
     3901 agttgcggac tgatgtacaa catgatgccc agtgcgccgg tgatgatgag cggctcgcgg
     3961 aataccgcct ccaatacgtt gaggatgctc cattccacct cctgcacatc agcggtgatg
     4021 cgcgacatga ggtcgccttt gcgctcttcc gagaagtacg acagcggcag caccagcagc
     4081 ttggcaaaca actgcccccg gaggtcgcgg atgataccgt tgcgcaccgg cgccataaaa
     4141 aacagggaga ggtagcgaaa gaggtttttc aaaaaaaaca cccccacaat ccagatgcac
     4201 acatacacca aagccgcctc tttgccttgt tcgacaatga ggcggctgac ggtatagccg
     4261 aaataatcgc tgatattttt gaaagaaaac cctgctgcag gcgcctccgt gatgagggct
     4321 tttcggtcga acagcagttc gaggaaaggc tgtagcagag gaatggcaat ggcggtgaac
     4381 aaagcggtca gcaggttgct cacgatgttc aataccacct gagagcggta attgcgcaaa
     4441 taaccgagca ggcgccagaa acttctcact actgcgtcag atcgaaatcg ttgagaaaac
     4501 cggtgtggaa attcccgttg cggaagttct cgtccttcat cagccgctga tggaagggca
     4561 cagtggtttt tacgccctca atgatgaact cgtccaatgc gcgctccatt ttgcgaatca
     4621 cttcttcgcg ggtgcgagcg cgacagatga gcttggcaat catcgaatcg tagaaaggcg
     4681 ggatggtgta gcctgcgtaa acgtgcgtgt ccacccgaac gccgtgccct ttggagctgt
     4741 ggaacgaact gatgcgcccc gggctgggcc ggaaattgtg gaacgggtct tcggcattga
     4801 tacggcactc catggcgtac atggtcgggt agtaattttt gccgctgatg cgttcgccag
     4861 ctgcaatttt aatttgctct ttgatgagat cgtggtcaat aacctcctcg gtcacaggat
     4921 gttccacctg aatacgggtg ttcatctcca tgaaatagaa gttgcggtat ttgtccacca
     4981 aaaactcaac ggtacccacg ccctcgtaat tgatgcactg accggccttg acggcagcct
     5041 cgcccatttt ttcgcgcagt tcgtcggtca taaacggaga gggactttcc tctaccaatt
     5101 tctggtggcg gcgctgtatg gagcagtcgc gctcggagag gtgcaccacc gtaccgaact
     5161 ggtcgccggc aatctgaaac tcaatgtggc gcggttcttc tacaaacttc tccacatata
     5221 caccgtcgtt accgaaagca gctttggcct cggtgcgggc cagagtgtag gcggtttcaa
     5281 aatctttttc ctcccaaacc acgcgcatgc ctttgccacc gcccccggca gtcgctttaa
     5341 tgatgacggg gtagccaatg tctttggcaa ttttatggcc gtgcttgagg tctttcacca
     5401 agccgtcgga acccggaacg accggcacgc cggccttgac catagtctcc ttggcggtga
     5461 ttttatcgcc cattttgcga atctggtctg cagaagggcc gataaatttg atgccgtatt
     5521 cgctgcaaac ctctgcaaac tctgcatttt cggccagaaa cccgtagccg gggtgaatgg
     5581 cgtcggcatt ggtgatttcc accgccgcca tgatgcgcgg gatgctgagg taagactcgg
     5641 aggaagcagg cgggccgata catacagcct cgtcggcaaa gcgcacgtgc aggctctcgc
     5701 ggtcggcggt agaataaacc gccaccgttt tgatgcccat ttcgcggcag gtgcgaatga
     5761 tgcgcagggc tatttcgccc cggttggcaa tgagtatttt attgaacata aggcgcttca
     5821 gtgatatgaa aaaaatctcc ctgacggatc gctcttacaa gacttatccc ttgggatcaa
     5881 tgaggaaaag cacctgatcg aactctaccg gagatgcgtc ttccaccata attttgacta
     5941 ccgtaccgcc gtgttcggat tcgatttcgt tgaacaactt catggcttcg atgatgcaca
     6001 ccacatcgcc gggcttgatg acgtcgccgg gcttcacata agccggtttg tcgggcgagg
     6061 gcgagcggta gaaggtgccc acaatcggcg agcgcacttc cagataagcg ccgctgcctt
     6121 tgtcggcaga tggtgcagat gnnnnnnnnn ncaggagccg atgttgattc ctgcacagga
     6181 gccggcgccg gggcgggagc cacagcagga gccgaaacca tgacaggtgc aacagcatgg
     6241 ccgcctctgt cgaagtgccg tgtgcggacg gacatttcaa attcgccatc cttcattttg
     6301 aactccgaca gctcgagttt gctgaccaag cggaggagtt cctggatttc cttgaagtta
     6361 ttcatgactg ttattgttta acccgttcca tgtatgcgcc gttgctggta tcaatgcgga
     6421 tgaaatcgcc ttcattgaca aacagcggca ctttgacatc tgcgccggtt tctactttgg
     6481 ccggcttgag ggtgttggtg gcagtatctc ctctgacgcc cggctctgtg taagtaacct
     6541 gcaaaaccac attggaagga agttccacat tgagcggaat gtctttttct gcgtggtaaa
     6601 ggatttccac ttcaatgcct tccttgagca attccgggcg ggagatcatg tcttccatga
     6661 tggaggtttg ctcgaaggta tcgttgtcca taaaatggta accggcatca tcggggtaaa
     6721 ggaactgaaa cttgcgacgc tctacgcgca ccacgtcaat tttgtggccg gaggggaaag
     6781 tgttttccaa aacgcgaccc gtggtaacgc ttttcatgcg ggtgcgaaca aacgcggcgc
     6841 ctttgccggg tttcacatgt tggaactcta caatggtgaa aatgtcgttg ttgtattcga
     6901 tacacaagcc gttacggata tcagctgtac ttgccataag tgtagtaaat attgatggtt
     6961 ttgaaaattt tgtgcaaaga tagaaaaaat cgggcggagc ctgtcaataa gcccatttta
     7021 gatacacagc gccccaggtg aagccgccgc cgaaagccgt gagtaccaga ttgtcgcctt
     7081 tttttagttg gttttcccac tcccacaggc acagcggtat ggtggctgcg gtggtgttgc
     7141 cgtagcgctc gatgttgatc atgacttttt caatcggaaa attgagttga ttggctaccg
     7201 actcgatgat gcgaaggttg gcctgatgcg gcactaccca agctacgtcg tcctggccca
     7261 attgattgcg ttccatcacc tgcctgacca catcggccat gccgttgacg gcgtatttga
     7321 acaccggttt gccgttttga tatgcaaaat gttcgcggtt gtctaaagtg tgttgcgagg
     7381 ccggccagcg cgatccgcct gctttttggt agaggtactg ctgcccca
//
LOCUS       DAORLF010000233         7395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5422_length_7395_cov_2.388011, whole genome shotgun sequence.
ACCESSION   DAORLF010000233 DAORLF010000000
VERSION     DAORLF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5422_length_7395_cov_2.388011"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PK198_16870"
     CDS             complement(<1..550)
                     /locus_tag="PK198_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF40468.1"
                     /translation="MKIAIIGAGIAGLAAAVRTASAGHEVDVFEANSYPGGKLSEFVQ
                     DGYRFDAGPSLFTMPQYVEDLFRAAGEDMEPHFQYQKLPVLCHYFWEDGTRLQASGKA
                     EQSAQDMEHIWGVKAEKVLAYLADSRQKYRLSGRIFLEKSLHKAGTWLNTGVIRSMLM
                     IPRFDLFTTMNAVNERYMDGNEK"
     gene            complement(675..2432)
                     /locus_tag="PK198_16875"
     CDS             complement(675..2432)
                     /locus_tag="PK198_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF40469.1"
                     /translation="MRQFALLLCLCLAVGATAQSAFLGIQSETISKKKAKVLGFTNLY
                     GSYVTKVIPGSPAEKAGLKPFDYIFGIDRLTAREDRDLSDMLSQYRAGDEATVYYMRK
                     NKSLKADVTFATRSSEPVASSQKKAFLGVSPFSNDDEDDEDPGVRVDIISNSSAAEMG
                     LKNGDRIMKINGYPMLDWDDISIAIGMLSAGDAIEVECEREGKVKTIEGKIKSYDESR
                     TKTEYTWAPKPQERAFLGITSNNVSSEKAAALGFTNAYGSYITEIIPGTAAEDAGLQP
                     FDYVHGINNDRTNEDNDLSALLAKYKAGDRATIHFIRKGKEQSKEVTFVSRNSARTVV
                     KNRCDKAFLGVSEGSWNEERPGQGVPVEIVANSTAAEMGLKDGDILLKINGYPILDWS
                     DVSAAVSNLKPRADITVTYRRGNAEIEHTLPVKSLCDTKGQEENEWNFNFDWKDLPGI
                     PNKNQQPAADEKYEPVNLGSATAGFKNLETAEAADLNTRYNLGLSADNSLTLEDVRLQ
                     PDPNIGRFRLQFRLPGSGLTNVRIFNATGRLIYEYELGVFSGEFSDDVDISQNGAGSY
                     YLEVRQGERSSTKKVTLQN"
     gene            2567..3712
                     /locus_tag="PK198_16880"
     CDS             2567..3712
                     /locus_tag="PK198_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF40470.1"
                     /translation="MTVETLENLELIQQSARDFAARYILPHVMEWDETQHFPVELFHE
                     MGHHGFMGVLVPHEYGGAGLGYQEYITIIEEIAKVCGSIGLSVAAHNSLCTNHILMFG
                     NEEQKQRYLPSLASAEWIGAWGLTEPNTGSDAGRMQCVAEKDGDYFIINGAKNFITHG
                     KSGQVAVVIVRTGELLDSRGMTAFVIEKGTPGFSAGKKENKLGMRASETTELIFDHCR
                     VHRSQMLGAEGEGFIQAMKVLDGGRISIAALSLGIAKGAYEAAVKYAKERHQFGKPIA
                     DFQAISFKLADMATQIQASELLVRKAGRLKDTGEKSTTLSAMAKYYASETAVRVANEA
                     VQVFGGYGYTKDFPVEKFYRDAKLCTIGEGTSEIQKLVISRELLKNG"
     gene            complement(3760..>4161)
                     /locus_tag="PK198_16885"
     CDS             complement(3760..>4161)
                     /locus_tag="PK198_16885"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656779.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRF40471.1"
                     /translation="GHRAGLDIVANIDAVPFKNGIPDWNAGGTTDPQEMVLITQSTKE
                     LKEIMSSYVGIVRTNMRLKRAMDRLHLLYTETEDLYNSTTISPQLCELRNLITIAYLI
                     TRSASMRRESRGLHYNTDFADKQEFIQTSLL"
     assembly_gap    4162..4267
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4268..5353)
                     /locus_tag="PK198_16890"
     CDS             complement(<4268..5353)
                     /locus_tag="PK198_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF40472.1"
                     /translation="MNQTDVLIIGSGIGGLSTAIKIAEQRPDLTITVLTKTVENESNT
                     RYAQGGVAAVWNEGIDSFDKHIEDTLDAGDGLCDLDTVRIVVTEGPERVREIIEWGAR
                     FDKTHSQQDYDLGREGGHSENRILHYKDLTGWEIQRALIAKADTLTNIVVLEHYFAID
                     IITQHHLGYVITRLTPGIECYGAYALNKRTNEIETFLARMTVVTTGGAGQVYRNTTNP
                     VIASGDGIAMVYRAKGWVENMEFVQFHPTALYNPAGENPVFLVSEAVRGFGGILKTRD
                     GEEFMHKYDPRKSLAPRDIVARAIDNEIKLRGEECMFLDCRHLDKEAFTAHFPTIYEK
                     CLSIGVNPMKDMIPVVPACHYMCGGIRT"
     assembly_gap    5571..5583
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5584..6653)
                     /locus_tag="PK198_16895"
     CDS             complement(<5584..6653)
                     /locus_tag="PK198_16895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF40473.1"
                     /translation="MRLNSALPTLLVVFSCLTFQAQHAAAAENVQYLKTKAQPGDGIL
                     SLMRRYLLEQHSCNFEQFYKLNNLKKNAPLLVGKDYTLPIALYSFDGRTIRSSVGISD
                     FNKALSIQHYNEKMVEAGICPESFKTSKVLWVPHHLLHCPDSDMVVTAPPASAVVPTL
                     ADPELSGGPRMFPIFGKDHAYVPLESSKLKGRVYYLIAGHGGPDPGAMTTRSGKKICE
                     DEYAYDVTLRLCKKLVAHGATAYVITRDENDGIRSAELLPCDTDETVWGGAAIPYGQK
                     ERLGQRTDIVNTLFEKHKAQGVSNQVLVEIHVDSRSHGRRTDVYFYHHSTSDSGKTLA
                     ENMQQTLKVKYNQVRKGEEYSGT"
     gene            6733..7392
                     /locus_tag="PK198_16900"
     CDS             6733..7392
                     /locus_tag="PK198_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRF40474.1"
                     /translation="MILLKQVGYLLRKELKLELRSNYAISGILLYVLATVFVVYRVFI
                     RLEPNVWNAMFWVVLLFASVNAVVKSFVQESGARQLYYYTLADPIAVILSKMIYNAAL
                     LLVISFLVWAGFVFFGGSPVKEMTLFLLALLLGSVGFSITFTFISAISAKADNNATLM
                     AILAFPLVIPILMTVLKLTAGALRLLRDTSVNNDMLILLGIDLLLLAMALVLYPFLWR
                     D"
BASE COUNT         1527 a         1866 c         2051 g         1832 t
ORIGIN      
        1 ccttttcgtt gccgtccatg tagcgttcgt tcacggcgtt catggtggta aacagatcaa
       61 agcggggaat catcagcatc gaacggatca cgcctgtgtt gagccatgtg ccggccttgt
      121 gcagtgattt ttccaaaaaa atgcgcccac tcaatcggta cttttgccgg ctgtcggcca
      181 gataagccaa tactttttcc gccttcacgc cccaaatgtg ctccatgtct tgcgccgact
      241 gttcggcctt accggaagcc tgcaagcgcg tgccgtcttc ccaaaaataa tggcagagca
      301 cgggcagttt ttggtactga aaatgtggct ccatatcttc gccggctgcg cggaacaggt
      361 cttctacata ttgcggcatg gtgaacagcg aggggcctgc atcgaaacgg tagccgtctt
      421 gcacaaattc ggagagtttt ccgccgggat agctgttggc ttcaaataca tccacctcgt
      481 gcccagccga ggcagtgcgc acggcggcgg ccaaaccggc aatacccgcg cctatgatcg
      541 ctattttcat acctgttttt gactgatttg cgataccaac aagccgggcc gggctttgtt
      601 gcgggcaaaa aaaagccgcc cccctttcga ggagcggcaa cgggacgata ttgagaaaat
      661 acttcgctga aatgtcaatt ttgtagggtg accttcttgg tagaagagcg ctcgccctgc
      721 cgcacctcca gataataact gccggcgccg ttttgcgaga tgtccacgtc gtcggagaac
      781 tcgccggaaa agacgcccaa ttcatattcg tagatcaacc tgccggttgc gttaaaaatg
      841 cgcacattag tgaggccgct gccgggcagg cggaattgca ggcggaagcg accaatgttc
      901 gggtcgggtt gcaatcgcac gtcttccagc gtcaggctgt tgtcggcgct caggcccaga
      961 ttatagcggg tattgaggtc ggccgcttcg gcggtttcaa ggtttttgaa gcccgcagtt
     1021 gccgagccga ggttgaccgg ctcatatttt tcgtctgcgg caggttgttg atttttattg
     1081 ggaataccgg gaaggtcttt ccagtcgaag ttgaaattcc attcgttttc ttcctgacct
     1141 ttggtgtcgc acagcgattt gacgggcagc gtatgttcaa tttccgcatt gcccctgcga
     1201 taggtgacgg tgatgtcggc gcggggcttc aggttggaaa cagcggcaga cacgtcgctc
     1261 cagtctaaaa ttgggtatcc gttgattttc agtaagatgt ctccgtcttt caggcccatt
     1321 tccgctgcgg tggaattggc cacgatctct accggaacgc cttgtccggg ccgctcctcg
     1381 ttccaggaac cttcgcttac gccgaggaag gccttatcgc aacgattttt gactaccgta
     1441 cgcgccgaat tgcgcgaaac aaaggtcacc tctttagact gctctttgcc cttgcggatg
     1501 aaatggatcg tggcacggtc gccggctttg tacttggcca gcagggcact gagatcgttg
     1561 tcttcattgg tgcgatcgtt gtttatacca tgcacataat caaaaggttg caggccggca
     1621 tcttcggcgg cggtacccgg aatgatttct gtgatgtagc tgccgtaggc attggtgaaa
     1681 ccgagtgcag cggctttttc ggacgaaaca ttgttggaag taatgcccaa aaaggcgcgt
     1741 tcctgcggct tcggtgccca ggtgtattcc gttttggtgc gcgattcatc ataagatttg
     1801 atcttgcctt caatggtctt gaccttgccc tcgcgttcgc attccacctc aatggcgtcg
     1861 ccggcggaaa gcataccgat ggcgatggag atatcatccc agtcgagcat cgggtagccg
     1921 ttgattttca taatgcggtc gccgtttttg aggcccattt cggcagcaga ggagttggag
     1981 ataatatcca ccctcacacc tgggtcttca tcatcttcgt cgtcattgct gaaaggcgac
     2041 acgccgagaa aagctttctt ttgagaagaa gctacaggct cagacgaccg ggtggcaaag
     2101 gtcacatccg ccttgagcga tttgtttttt ctcatgtaat aaaccgtcgc ctcgtcaccg
     2161 gcgcggtatt gcgaaagcat atcgctgaga tcgcggtctt cgcgagccgt cagtcggtca
     2221 atgccgaaaa tatagtcaaa cggcttcagt ccggcttttt ctgccggtga gccgggaatg
     2281 accttagtga cgtagctgcc gtataaattg gtgaatccga gcactttggc tttcttcttg
     2341 gaaatggttt cagactgaat tccgagaaaa gccgattggg ctgtagcgcc gacggcgagg
     2401 cacaggcata gcaaaagggc gaactgtctc atatctttcc gtttgtttag gtttggggta
     2461 atgacaagca tacttcaaag aggttgcata gatttgcgct tcgtttgaac tttttactat
     2521 ttttcagctt ttagaaatcc gaagccactc atcaaataac caaataatga ccgtagaaac
     2581 acttgaaaat ctggaactta tccagcaaag cgcccgcgat tttgccgccc gttacatctt
     2641 gccccacgtc atggaatggg acgaaacgca acatttccct gtcgaactgt tccacgaaat
     2701 gggccatcat ggcttcatgg gcgtgcttgt gccgcatgag tacggcggcg caggcttggg
     2761 ttatcaggaa tacatcacca tcattgagga gattgccaag gtatgcggct ctatcggcct
     2821 gtcggtagct gcgcacaatt ccttgtgtac caatcatatt ctgatgttcg gcaacgaaga
     2881 acaaaagcag cgctacctgc cctcgctggc ttccgccgaa tggatcggcg cctggggcct
     2941 tacggaaccc aacaccggca gcgacgcggg gcgcatgcaa tgcgtggcag aaaaagatgg
     3001 agattatttc atcatcaacg gggccaaaaa cttcatcaca cacggcaaat cggggcaagt
     3061 agccgtcgtc atcgtgcgca ccggcgaact gcttgacagc cggggcatga cggccttcgt
     3121 catcgaaaaa ggcacgccgg gtttcagcgc cggcaaaaag gaaaataagc tgggcatgcg
     3181 cgcctcggaa actaccgaac tcattttcga ccattgccgg gtgcatcgca gtcagatgtt
     3241 gggtgctgag ggcgagggtt ttatacaagc catgaaagta cttgacggcg gccgtatttc
     3301 cattgcagcc ctgtcattgg gcattgccaa aggcgcttat gaggcggcgg tgaaatacgc
     3361 caaagagcgg catcagttcg gcaagcccat tgccgatttt caggccatca gtttcaaact
     3421 tgccgacatg gccacccaga ttcaggcctc agaactgctt gtccgcaagg ccgggcgact
     3481 gaaagacacg ggcgaaaaaa gcaccacgct gtcggctatg gccaagtatt acgcttcgga
     3541 gactgcggtg cgcgttgcca acgaagccgt gcaggttttc ggcggctacg gctataccaa
     3601 ggattttccg gtggaaaaat tttaccgcga cgccaaactc tgcaccatcg gcgagggtac
     3661 ttccgaaatt cagaagttgg tcatttccag agagttgctg aagaacgggt agcggactca
     3721 tgtttggcga aaatgaccac tttatttttt tatcgcccct tacaacaacg aggtctgtat
     3781 aaattcctgt ttgtcggcaa aatccgtatt gtaatgcagg ccccggcttt cgcggcgcat
     3841 ggatgccgag cgggtaatga ggtaagcgat ggtgatgaga ttgcgcagtt cgcacagttg
     3901 cggagagatg gtggtgctgt tgtacaaatc ctccgtttcg gtgtagagca ggtgcaggcg
     3961 gtccatggcg cgtttgaggc gcatattggt gcgcacaatg cccacatagc tgctcataat
     4021 ttccttcaac tctttggtac tttgcgtgat gagcaccatc tcctgcgggt cggtggtgcc
     4081 gccggcattc caatcgggga tgccgttttt gaaaggcacg gcatcaatgt tggccactat
     4141 gtcgaggccg gcgcgatgcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnggt acgaatgccg ccgcacatgt aatggcaggc cggaaccacg ggaatcatgt
     4321 ctttcatggg gttcacgccg atgctgaggc acttttcgta aatggtgggg aagtgcgccg
     4381 tgaacgcctc tttgtccaaa tgccggcaat cgaggaacat gcactcctcg ccgcgcagtt
     4441 tgatttcgtt gtcaatggcg cgggccacga tgtcgcgggg ggccagcgat ttgcgggggt
     4501 cgtatttgtg cataaactcc tctccgtcac gggttttgag aatgccgccg aaaccgcgca
     4561 ccgcttctga aactaaaaac accgggtttt cgccggccgg attatacagc gccgtagggt
     4621 gaaactgcac aaactccatg ttttccaccc agcctttggc gcgataaacc atggcaatgc
     4681 cgtcgccgct ggcaatgacc ggattggtgg tgttgcgata tacctgtcct gcgccgccgg
     4741 tggtcacgac ggtcattctg gcgaggaagg tttcgatttc attggtgcgt ttgttcagcg
     4801 cataggcgcc gtagcactcg atgccgggcg tgagccgggt gatgacgtag ccgaggtgat
     4861 gctgcgtgat gatgtctatg gcgaagtagt gctccagcac cacgatgttg gtcagggtat
     4921 cggctttggc aatgagggcg cgttgtattt cccagccggt gaggtctttg tagtgcagga
     4981 tgcggttttc ggaatggccg ccttcgcgcc ccaggtcgta gtcttgttgc gaatgggttt
     5041 tatcgaagcg ggcgccccac tcaatgattt cgcggacgcg ctcaggccct tcagtcacaa
     5101 cgatgcgcac ggtgtcgaga tcgcaaaggc cgtcgccggc atccagggtg tcttcgatgt
     5161 gcttgtcgaa ggagtctatg ccttcattcc acaccgccgc tacgccaccc tgagcatatc
     5221 gcgtattgct ttcgttttca acggtttttg tcaggacggt tatggtgaga tcggggcgtt
     5281 gttccgcaat cttgatggca gtactcagcc cgccgatacc ggagcctatg atgagaacgt
     5341 ctgtttggtt cataagatag ctttgttaag aggaggcaaa ggtaggaaag cacactattt
     5401 gtcaaagaag gagaactatt tcgtaagccc ctcaaacaac cactctgcca agagttgccg
     5461 gttggaaggc agtacgatgc gctgctgatc gccgtagttt ttaatgttgg ccagttctat
     5521 atacacaccg gtcggcttgg cttcgcggag ggtgaacaaa tctcgcgccg nnnnnnnnnn
     5581 nnngtgccgc tgtattcctc gcctttgcgc acctgattgt attttacttt gagggtttgc
     5641 tgcatgtttt cggccagcgt tttgccgctg tcgctggtgg agtgatgata aaaatacacg
     5701 tcggtgcggc gcccatgact tctggagtcc acatgaattt ctaccaacac ctgattggaa
     5761 acaccttgcg ccttgtgttt ttcaaagagc gtgttcacta tgtcggtgcg ctgccccagg
     5821 cgttcttttt gcccgtaagg aatggccgcg ccgccccaca cggtttcgtc ggtatcgcag
     5881 ggcaatagtt ctgcgctgcg gatgccgtcg ttttcatcgc gggtgatgac ataagccgtt
     5941 gctccgtggg ccacgagttt tttacacaat cgcagcgtca catcataggc gtattcgtct
     6001 tcgcagattt ttttccccga acgggtagtc attgcgccgg ggtcggggcc gccgtgcccg
     6061 gcaatgaggt aatagacccg gcctttgagc ttgctgcttt ccaaaggtac ataggcgtgg
     6121 tctttgccga aaatgggaaa catgcgcggc ccgcccgaca actcaggatc ggccaatgtg
     6181 ggaactaccg ctgatgccgg cggcgccgtg actaccatat ccgaatcggg gcaatgcagc
     6241 agatgatgcg gcacccagag tactttgctg gttttgaacg attccgggca gatgccggcc
     6301 tccaccattt tttcattgta atgctgtatg ctcagcgcct tgttgaagtc gctgatgccc
     6361 accgaggagc ggatggtgcg cccgtcgaaa ctgtacaacg ctatgggcag cgtgtagtct
     6421 ttgccgacca gcaaaggggc gtttttttta aggttgttga gcttgtaaaa ctgctcgaaa
     6481 ttgcaggagt gttgctccaa caggtatcgc cgcatcagcg acaggatgcc gtcgccgggc
     6541 tgggctttgg ttttgaggta ctgcacgttt tcggcagcag cggcatgttg agcctgaaaa
     6601 gtgaggcaag aaaaaacaac cagaagagtc ggcagtgcag aatttaggcg cattgaaaca
     6661 gttttcgttt ttttggaacg agcgcaaaaa tagcaaattg gctgtgttgc cgaaaggctg
     6721 taccttcgcc gcatgatttt attgaaacaa gtgggctacc tcctgcgcaa ggaattgaag
     6781 ctggaactgc gctccaacta cgccatcagc ggcatcctgc tgtatgtgct ggctaccgtg
     6841 tttgtggtgt accgggtgtt catccggctg gaacctaacg tgtggaatgc catgttttgg
     6901 gtggttttgc tgttcgcatc ggtcaatgcg gtggtcaaaa gttttgtgca ggaaagcggc
     6961 gcccggcagt tgtattacta taccctggcc gatccgattg cggtcatttt atccaaaatg
     7021 atctacaatg ccgcgctcct gctcgtcatc agttttttgg tatgggccgg ttttgtgttc
     7081 ttcggcggca gcccggtcaa agaaatgacg ctgtttttgc tggcgctctt gctgggcagc
     7141 gtgggctttt ccattacgtt cacgttcatt tccgccattt ctgccaaagc cgacaacaac
     7201 gccacgctga tggccattct ggcattccct ttggttattc ccattcttat gacggtgctc
     7261 aaactcaccg ccggcgcact ccggctcctg cgagatacca gcgtcaacaa cgacatgctc
     7321 atcctgctgg gcattgacct gttgctgctg gctatggcct tagtgctgta cccgtttttg
     7381 tggcgggatt aaggg
//
LOCUS       DAORLF010000234         7319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5495_length_7319_cov_1.752753, whole genome shotgun sequence.
ACCESSION   DAORLF010000234 DAORLF010000000
VERSION     DAORLF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_7319_cov_1.752753"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>781)
                     /locus_tag="PK198_16905"
     CDS             complement(<1..>781)
                     /locus_tag="PK198_16905"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HRF40475.1"
                     /translation="SVPLTASTLAIGQDRLLRIQDACQCPVGLENLAFAFSLEEVRQH
                     GAFLEQLLEPVNGFIILDLHNLYCQMKNFDLGFDALVHSYPLHRVREIHISGGSWEPS
                     TIIPTRNIRRDTHDDAVPEEVFEMLAQTIALCPALKFIFLEQLGAGLHTVSQQSQFRS
                     DYLRICEIVKKHAPAVAQGDAESFSPRPNLQPPSAHIQDAVLYEEQRRLSRILETASD
                     CQDARQKLESSILKNSAWQTEAWPDYMLETALRIAQKWQDGF"
     assembly_gap    782..903
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<904..1139)
                     /locus_tag="PK198_16910"
     CDS             complement(<904..1139)
                     /locus_tag="PK198_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40476.1"
                     /translation="MSPSIHTGIACNLDSNIITAALPLLEQEHVEAIEWSFDALFNWN
                     EVPAWFRDLLQVFADAGRLTGHGIFFSIFSARWSA"
     assembly_gap    1305..1319
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1600..2286)
                     /locus_tag="PK198_16915"
     CDS             complement(1600..2286)
                     /locus_tag="PK198_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40477.1"
                     /translation="MRYAILLLAAVITMTACRQNSPDTTTVVAPPTPQNTIEQPASAA
                     SADLGSAEDTPNLPVLELSYFQPNKDGSGIAGVPRPDKVEKPPKGKLISYKYFKAHII
                     PRADGPGEDINVYQPEGGLAYIGGEAAYYAGIYNQYLLLDVGTSASKRQIMAINMVSG
                     DTTYRADYHGDYVAMYGQYLVHLSADPNASDCQVKGKNLPGKMHRAYWVNLVSAKPKA
                     SAQQECLYVE"
     gene            complement(2347..>2759)
                     /locus_tag="PK198_16920"
     CDS             complement(2347..>2759)
                     /locus_tag="PK198_16920"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF40478.1"
                     /translation="LVGNEANAIQPGKRMLSSMTPTIVEKDGQLFMVLGAPGGSTIIT
                     AVFQVLLNVVEFNLDLESAVNAGRFHHQWLPDQIMVEEAALSPQVRQALTDKGHKLQS
                     VKYMAVIKAIQRLPDGRLIGAGDPRNPDDDAEGE"
     assembly_gap    2760..2935
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2936..>3200)
                     /locus_tag="PK198_16925"
     CDS             complement(<2936..>3200)
                     /locus_tag="PK198_16925"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF40479.1"
                     /translation="QMLEMVEPYPLGQYGFQSAEAVHLMAEAERRAYADRAEYLGDTD
                     FYKVPMDSLLNETYLRGRMADFNPAKAGQSAALGAGKFQLTKES"
     assembly_gap    3201..3364
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3365..>3773)
                     /locus_tag="PK198_16930"
     CDS             complement(<3365..>3773)
                     /locus_tag="PK198_16930"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF40480.1"
                     /translation="AIDYREKAPLAAHRDMYLDSLGQVMPRLSQDGHLAAGVPGTPAG
                     LLEVHRKYGRLPMAQLMARAIEYADKGFSISEGEAARLNSFQEAYRKQNTAPTVFIKD
                     QWKAGDRLVQKDLAAVLKRIRKQGAAGFYEGKTA"
     assembly_gap    3774..3883
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4239..5132)
                     /locus_tag="PK198_16935"
     CDS             complement(4239..5132)
                     /locus_tag="PK198_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRF40481.1"
                     /translation="MKNQITMFDMGEQSIERSIINVASVPQRSPFRYPGGKTWLIPTV
                     RQWLKQENKIVKELIEPFAGGGIVSLTAAFEKMAEQIIMVELDEEIAAVWEVILNEKN
                     KWLADKIYSYDLTHANVKAELENPNKQLQDIAFCTILKNRVFHGGILAKGSGLIKNGE
                     NGKGIASRWYPKTLRDRILAINHVKDKIQFILGDAFKILEQNLNNENAYFFIDPPYTI
                     AGKRLYTYFDINHEKLFELTSQLKGKFMLTYDDTPEIRQLADKYYLQYRTIPMKTTLH
                     FEKNEIIISDNFSWWRGSNSR"
     gene            complement(5129..6013)
                     /locus_tag="PK198_16940"
     CDS             complement(5129..6013)
                     /locus_tag="PK198_16940"
                     /inference="COORDINATES: protein motif:HMM:NF023605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HRF40482.1"
                     /translation="MSLDKKEIQPLGEVFGFPIDNESDRAKRYRSNKLCPYNNIVSNC
                     TKNSIEFPLGVCSLNYKGKQVIICPIRFREDWIIISDAANFIFDGKATWTHVGEVRLA
                     DKHGKSAGNIDYVLVSYDDNGRVLDFGSLEVQAVYISGNLTGPFTAYLDAPSPDFSWT
                     KAFKYPKPDYLSSSRKRLIPQIIAKGSILKQWNKKQVVALQTNFYNTLPVLPEFEKEE
                     SDFAFFLYDLVPDKKTKALSLKLQRIIYTKFASALEQIAKFEAGSINQFTEILQKKLD
                     AKRAGASDINNLENIVVE"
     gene            6466..>6714
                     /locus_tag="PK198_16945"
     CDS             6466..>6714
                     /locus_tag="PK198_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40483.1"
                     /translation="MEKGKKFKYPGTRVALDGNTAAIMCERESTDAAGAYPITPSTQM
                     GEYWAEEAAKGHLNISDRPLIFIEPEGEHAAAAVTAGLS"
     assembly_gap    6715..6725
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6726..>7319
                     /locus_tag="PK198_16950"
     CDS             <6726..>7319
                     /locus_tag="PK198_16950"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40484.1"
                     /translation="MTGLRAANFSSGQGIAYMHESLYAAVGKRLTYVLNIGARAMTKS
                     TLNVHAGHDDYHAIDDTGFFQLFANKAQHVADLNIITHRIAELSLTPGAIAQDGFLTT
                     HLIESLLIPERELISEFLGRPDDIIETPTPAQRLIYGPTRRRIPEVWDVDNPMMAGIV
                     QNQDAYMQSVAAQRPYFFDHIQKLTDQFIADIEEMTKD"
BASE COUNT         1561 a         1618 c         1741 g         1801 t
ORIGIN      
        1 aaaaccgtct tgccactttt gcgcaatgcg caaagccgtt tcgagcatat agtctggcca
       61 cgcttcagtt tgccaggctg agttcttcaa aatagaagat tccaattttt gccgggcatc
      121 ctggcaatcg gaggccgtct caagaatgcg agagagccgg cgttgctcct cgtacagaac
      181 agcatcctga atgtgcgcag aaggaggttg taggttgggc cggggagaaa aactttcagc
      241 atcgccttgt gcaacagccg gggcgtgttt ttttacaatt tcgcaaatac gaaggtaatc
      301 gctcctgaat tgagattgtt gtgaaacagt atgcaggcct gctcctaatt gctccaaaaa
      361 aatgaattta agggcggggc aaagtgctat ggtttgagcg agcatttcga atacttcttc
      421 aggaacggcg tcgtcgtgcg tatctctgcg aatgtttcgt gtcggtatga tcgttgaagg
      481 ttcccaactg ccgcctgaaa tgtgtatttc ccgcactcga tggagcggat aggaatgaac
      541 aagcgcatca aaccccaagt cgaaattttt catttggcag tacagattgt gcagatcgag
      601 gatgataaac ccgtttacgg gttcgaggag ttgttccaaa aatgctccat gctgtcttac
      661 ctcttccagt gaaaatgcaa aggcgaggtt ttcaagcccg accgggcatt ggcaggcatc
      721 ctgaatgcgg agtagccggt cttgcccgat ggccagtgta cttgccgtca aaggtacgct
      781 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnngctgacc aacgtgcaga aaaaatggag aaaaaaattc catgacctgt cagtcggccg
      961 gcatcggcga aaacttgcag caaatcccga aaccatgcag gaacttcgtt ccaattaaac
     1021 aaagcgtcaa aactccactc aatggcctct acatgttctt gctccaatag cgggagcgct
     1081 gcggtaatga tattagaatc aagattgcag gcaattcctg tatgaatgct tggggacatg
     1141 gccttatttt agcccagacc gcaggcgggg cagttgtaat ctggttgccg ctttaggaca
     1201 tggacgcagg tctcttcgtt tttacaggat tcgtcgcaat cactggtcgt gatttctact
     1261 ttatcgcagg aggtggcgac ggtggcggta gctgcgatgc cgatnnnnnn nnnnnnnnng
     1321 ctttcaaaag ttcttttggt aatttcatgg caagatgttt ttattgaaaa atgatatact
     1381 tccctgcaag acgtaaagag ggagccgaga ggttgggttg aaaataaaag atggcgcggc
     1441 gcaattgctg cttgttgcgg caggggcgga agtggtactg ccgtcgtctt aacgcggcag
     1501 taagaacgga cgacccggcg cctgagcgag tggccccgcc ggggtgcatt tgcgggagcg
     1561 gggctgggtg agacaggcgg acgcccccaa aaaaaacaat cattctacat acaggcattc
     1621 ttgttgtgca gacgccttgg gtttggccga tacaaggttg acccagtaag cgcggtgcat
     1681 tttgcccggc agatttttac cttttacctg gcagtcggag gcattgggat cggcgctgag
     1741 gtgaacgagg tactggccgt acatggctac ataatcgccg tgataatcag cacgataggt
     1801 tgtatctcca gagaccatgt tgatggccat gatttgccgt ttcgaagctg aagttcccac
     1861 atcgagcagg aggtattggt tgtagatgcc cgcataatat gcggcttctc cgccgatgta
     1921 tgccaggccg ccttccggtt gatagacgtt gatgtcttcg ccggggccgt cggcgcgcgg
     1981 aatgatatgc gctttgaagt atttatagct gatgagtttt cctttgggcg gcttttctac
     2041 cttatcgggg cggggtacgc cggcaatacc tgaaccgtct ttgttgggtt ggaaataact
     2101 gagttccaac acgggcaaat tgggggtgtc ttcggcactg cccagatcgg ctgaagccgc
     2161 tgaggccggc tgttcgatgg tattctgtgg cgttggcggg gcaacgacgg ttgttgtatc
     2221 aggtgaattt tggcggcagg cggtcattgt gatgacggct gcgagaagta aaattgcgta
     2281 tcgcatacgg tagttgtttg aggaggcgaa gataaggaac ggggtcggtt cacggtgcta
     2341 aaaatattac tctccttctg catcgtcgtc cggattgcgc ggatctccgg cgccgatgag
     2401 ccgcccgtcg ggcaggcgtt gtatggcttt gatgaccgcc atgtatttca ccgattgcag
     2461 cttgtgccct ttgtcggtca gtgcctgccg cacctgcggg ctgagcgcgg cctcttctac
     2521 catgatctga tcggggagcc attggtggtg aaaacgcccg gcgttgactg ccgattcgag
     2581 gtcgagattg aattccacca cgttgaggag cacctgaaac actgccgtga tgatggtgga
     2641 gccgcccgga gcacccagta ccatgaagag ttgaccgtct ttttccacga tggtgggcgt
     2701 catggagctg agcatgcgct tgccgggctg tatggcgttg gcttcgttgc ctaccaagcn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactct
     2941 cttttgtcag ttgaaatttg cccgcaccga gcgcggcgct ttggcctgct ttggcggggt
     3001 tgaaatcggc catgcggcca cgcaggtagg tttcgttcag gaggctgtcc atcggcactt
     3061 tgtaaaagtc agtatcgccc agatactcgg cgcggtcggc ataagcgcgg cgctctgcct
     3121 cggccatgag gtgtacggct tccgccgatt ggaacccgta ctgccccagc ggataaggct
     3181 ccaccatttc cagcatttgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnngcggtt ttgccctcgt agaagcccgc tgcgccttgt ttgcggatgc gcttgagcac
     3421 ggctgcgagg tctttttgca ctaagcggtc gccggctttc cattggtctt tgatgaacac
     3481 ggtgggcgcg gtattctgtt tgcggtacgc ctcctggaac gaattgagcc gggcggcttc
     3541 tccctccgag atagaaaaac ctttgtcggc gtactcgatg gcgcgcgcca tgagctgcgc
     3601 catgggcagg cggccatatt ttcgatgtac ttccagcagt ccggcgggtg tgccgggcac
     3661 gcccgctgcg aggtgtccgt cttggctcag gcggggcatc acctgtccga ggctgtctaa
     3721 atacatatcg cggtgggcgg cgaggggcgc tttttcgcgg tagtcaatgg ctgnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccttgt tttaaaattt
     3901 ccacgccggc ggcagaggcc aatggatgcg gggccactac cataccgcgt tggctttcag
     3961 cggttttggt ggcggaataa acggaaccgg gcaatgcccg ctgcgcccag gcgacctggg
     4021 caaggagggt aaaaacgagg aggaggcgcg cgattttcat ttttggcacg gttgttggtt
     4081 tatataaaga gtggcgaata tagatggata gaacagcttg attcgctgcg caagatcgca
     4141 aaactcgttc gttaaagata aacaagggag atttttcaga gggcgccgcc tgggtttcag
     4201 agcggcgtct tttttttggg ggggcggggt agatgctgtt agcgtgagtt gctacctctc
     4261 caccatgaga agttgtctga aataatgatt tcgttctttt caaagtgaag cgttgtcttc
     4321 attggaatcg tcctatattg caaatagtat ttgtctgcaa gctgccgaat ttctggtgtg
     4381 tcatcatatg ttaacatgaa ttttcctttg agttgagaag tcagttcaaa aagcttttca
     4441 tgattgatgt caaaatatgt gtaaagtctt ttgcctgcaa ttgtataagg tggatcaata
     4501 aaaaagtacg cattttcatt attgagattc tgttctaata ttttaaatgc gtcacccaaa
     4561 atgaattgaa ttttgtcctt tacatggttg attgcaagta ttctatcacg taaggttttt
     4621 ggataccaac gtgacgcgat tcctttgccg ttttctccat tcttaatcag ccccgagcct
     4681 ttggcaagaa taccgccatg aaaaactcta ttttttaaaa tggtacaaaa tgcaatgtct
     4741 tgaagttgtt tatttggatt ctctaattca gcttttacgt ttgcatgtgt caagtcgtat
     4801 gaataaatct tgtcggccag ccacttgttt ttttcgttta aaatcacttc ccaaactgct
     4861 gctatttctt cgtccaattc taccattata atttgctcag ccattttttc aaatgcagca
     4921 gtcagactta caatgccccc acccgcaaaa ggctcgatca gttcctttac gatcttgttt
     4981 tcctgtttca accattgtct aactgttgga attaaccaag tcttgcctcc cggataacga
     5041 aacggactgc gttgaggcac agatgcaacg ttgatgatag atcgctcgat tgattgttct
     5101 cccatgtcga acatagttat ttgatttttc attctacaac tatgttttca aggttattga
     5161 tgtcagaagc acctgctctt ttagcatcaa gtttcttctg taatatttct gtgaactggt
     5221 ttatagaacc tgcttcaaat tttgcaatct gctctaaagc gcttgcgaat ttggtgtaaa
     5281 taattcgttg aagtttcaac gaaagcgctt tcgttttctt gtcaggcacg aggtcgtaga
     5341 gaaaaaaagc aaaatccgac tcttctttct caaattcggg aagtacaggc aatgtgttgt
     5401 aaaagttcgt ctgcaatgca acaacttgct ttttgttcca ttgctttaaa atggagcctt
     5461 tggcaataat ttgaggaatt aatctttttc ttgatgacga aagatagtcc ggctttggat
     5521 atttgaaggc ctttgtccag ctaaagtctg ggcttggagc gtccaaatat gcagtaaaag
     5581 gacctgttaa gttgcctgaa atgtaaacag cttgaacttc aagcgatcca aagtctaata
     5641 ctcgcccgtt gtcgtcataa gaaactaaaa cgtagtcaat attacccgca gatttgccgt
     5701 gcttgtctgc cagtcttact tcaccaacat gtgtccaagt tgctttgcca tcaaaaatga
     5761 agttggccgc atcacttatt attatccaat cttctcgaaa tcttatagga catataatga
     5821 cttgtttgcc tttatagttt aggctacaaa cacctaatgg aaactcaata ctgttcttag
     5881 tacagtttga aacaatgtta ttgtatgggc atagtttgtt gctccgataa cgttttgctc
     5941 tatcgctttc attatcaatc ggaaagccga aaacttcgcc taaaggttgt atttcttttt
     6001 tatccaaact cattcgccaa atttataata tttttttatt tttaagtcag accgcgagag
     6061 cacatgtatg ttcggtaaga gagtctttgt aatttctgct aactaataca ttgcagctat
     6121 cccctacaca ccccctatcg cctttatctc tccgcttttt cttcaccccc tccttacttc
     6181 caatggcttt cacggcgatc agttcacttt ctccgggatt caaaaagcgt agaacggaag
     6241 caaagataaa ggcttcgttt ggaaaaagaa atgcagcgct gtaaaaattg cccccgcccc
     6301 ttaggactta tgtttgccgg aaaggctttt gcgcgagttg ccggttgagc gactgacaaa
     6361 gatcaaacgc cccgctgacc aaagtcatgc ccctgtaccc tgtgcccgct gtaatcttgc
     6421 agaagatttt ttttaccgaa aagtacttct acaaaagtag cgatcatgga aaaaggcaaa
     6481 aagtttaaat atcccggcac gcgggtggca ttggacggca acacggcggc cattatgtgc
     6541 gaacgggaat caaccgatgc agcgggcgcg tatcccatca ccccctccac gcagatgggc
     6601 gagtattggg cggaagaggc ggccaaaggg cacctcaaca tctccgaccg gcctttgatt
     6661 tttatcgaac ccgaaggcga acacgctgcg gctgccgtca cggcgggctt gtctnnnnnn
     6721 nnnnnatgac ggggctgcgg gcggccaatt tctcctccgg ccagggcatc gcgtacatgc
     6781 acgaatcgct gtacgccgcg gtgggcaaac gcctgacgta tgtgctcaac atcggcgcca
     6841 gggccatgac caaatccacc ctgaacgtac acgccggcca tgacgactac catgccatag
     6901 acgatacggg cttcttccag ttgtttgcca acaaagccca gcatgtggcc gacctcaaca
     6961 tcatcaccca ccgcattgcc gagttgtcgc tcacgccggg cgccatcgcg caggacggct
     7021 tcctgaccac gcacctcatc gagtcgctgc tcatacccga acgcgaactc atcagcgagt
     7081 ttctgggccg ccccgacgac atcatcgaaa cgccgactcc cgcgcagcgc ctcatttacg
     7141 gccccacccg ccgccgcatt cccgaagtgt gggacgtgga caatcccatg atggccggta
     7201 tcgtgcaaaa ccaggatgcc tacatgcaaa gcgtggcagc gcagcggccc tacttcttcg
     7261 atcacatcca gaagctgacc gatcagttca tcgccgacat cgaagaaatg accaaggat
//
LOCUS       DAORLF010000235         7307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5508_length_7307_cov_2.212217, whole genome shotgun sequence.
ACCESSION   DAORLF010000235 DAORLF010000000
VERSION     DAORLF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_7307_cov_2.212217"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..797)
                     /locus_tag="PK198_16955"
     CDS             complement(27..797)
                     /locus_tag="PK198_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRF40485.1"
                     /translation="MNAFTAADLVHRSAFVIRSYEIDPHKIASAPALVRIMQEASMEH
                     VMRLQLSVWDLEPRGLSWVLMRQRLHFERLPRLGETVEIITHPSAFERVFTHRDYRMY
                     DAAGQEIAQASTTWLLMQTATRRMARIPADMLAYNELLPASDSHLARAAEVLPEFGTA
                     SNAIRFTIHWFDLDFNFHLTNSKYVEWLLASADEDLLRDAALSDMEVHYLAECTLGEE
                     VISETATEQENIRMHRLVRASDGKALAKARSVWKELTA"
     gene            complement(805..1257)
                     /locus_tag="PK198_16960"
     CDS             complement(805..1257)
                     /locus_tag="PK198_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40486.1"
                     /translation="MKSLLKLVAVVVVGVLIYNYFFGTPEEKATSKEIFTDVRDLGKA
                     TWGLLKSEKQKFDEGKYDEAVDKVGGLLGSLKSKAKSSDDDASLAKLEELERRRLELE
                     QEVVALEQLNKSGKTVAAKAKEDQIRKDWRTLMDATELVMNNLEKAPR"
     gene            complement(1284..>2102)
                     /locus_tag="PK198_16965"
     CDS             complement(1284..>2102)
                     /locus_tag="PK198_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF40487.1"
                     /translation="REDSIVTAYRQHGTALGRGVSSREAMAELFGKETGIVKGKGGSM
                     HFFSKPLNYYGGNGIVGAQIPIGTGIAFAEKYKGTDNLCVTLFGDGASRQGALHESFN
                     MAMAWKIPVLYVVENNGYAMGTSVGRTSNVVELYKLGASYDMPSEAVDGMNPEAVHEA
                     VTRAAEHIRAGNGPYFLEIRTYRYKGHSVSDPAKYRTKEEVESYKERDPIKLIESKIL
                     ENGIATEEEIQAIRDRVKAEIDDCVEFAENSEYPAGSTLYEDNYVQADYPYIRD"
     assembly_gap    2103..2128
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2129..2326)
                     /locus_tag="PK198_16970"
     CDS             complement(<2129..2326)
                     /locus_tag="PK198_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF40488.1"
                     /translation="MVARKKKATGGMQHPKDRYMYWYELMLRIRRFEERALVMYGQQK
                     IRGFCHVYIGQEAVAAGIESAI"
     gene            complement(2423..>3158)
                     /locus_tag="PK198_16975"
     CDS             complement(2423..>3158)
                     /locus_tag="PK198_16975"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRF40489.1"
                     /translation="ALSRRKRFYTQDLNRMWRPDLINRLKHTHPADLRYEERELRELA
                     EAVEREIAMYRPEKVIVLDLHTTTADGGIFGIATDDPESIRIAQELHAPVITGMLAGL
                     QGTMLHYFNKENIQAPEVVALGFEAGQHDDPLSVNRCIAAIINCMRTIGCVDADDVEN
                     RHDDLLIAYSKGLPKVAALRYVHHIQPQDEFRMTPDYHNFKSVKQDEILAHDRFGPIK
                     AKEDSLILMPLYQAQGSDGFFLVKEV"
     assembly_gap    3159..3207
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3350..>4002)
                     /locus_tag="PK198_16980"
     CDS             complement(3350..>4002)
                     /locus_tag="PK198_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40490.1"
                     /translation="PVALLIIHTFAPMSDLILSHLSADPAMARVIRETIDKPDFRQGN
                     SDVYYDLLVSIISQQLSGKVAQVISGRFMALFSNQYPEPEDVLAMEFEQLRAVGLSNS
                     KTHYIRNVAEFFSSPEHRGKNWSEMSDEDIIRFLTQIKGVGRWTAEMVLMFTLNRTDV
                     LPLDDLGIQQSMAQLFGWQESGKALHKKMVEAAERWRPYRTYACKFLWRWPRNTTK"
     assembly_gap    4003..4119
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4120..5623)
                     /locus_tag="PK198_16985"
     CDS             complement(<4120..5623)
                     /locus_tag="PK198_16985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRF40491.1"
                     /translation="MRKTNLHIALILLFGLASQLSLNALSDKIRCTWRDNPATSMVIA
                     WDQVQGANPVVYFDVVDQGRNFNAYKLSRKPDRVIFAKGMNNHFVRLSGLQPNTAYYF
                     IIKDSEGSTARYSFRTAPDQPTQRLSIIAGGDSRNHREARKSANAMVAKLRPHFVLFN
                     GDMTVNDSSTEWEDWLKDWQLTIAADGHITPVLVARGNHESENKTLIDMFDLAHPGNF
                     YAFNFGGNLVRIYTLNTLIPAGGEQKNWLENDLKTTGNSVVWKMAQYHHAIRPHTAAK
                     TEKDDIYLAWAELFHEHGLKLAIESDSHVAKWTYPLRPDTGPGSDMGFIRDDATGTVF
                     VGEGCWGAPLRENDDDKSWTRASGSFNHFNWIFIDQEKIEVRSVMTDNAAQAGTVSLN
                     NIFEPPLGLSLWSPPGGDVIRIYKKKPPVAESASTKERPQHTTAPAPAESDDLGGNWD
                     ALPRLVPNPASGKVKIDYEIAQQADVTLLLVNAQKQEMARLEYPAQPPGPQ"
     gene            5729..7009
                     /gene="glyA"
                     /locus_tag="PK198_16990"
     CDS             5729..7009
                     /gene="glyA"
                     /locus_tag="PK198_16990"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRF40492.1"
                     /translation="MTKDTALFDLIHEELTRQRKGIELIASENFTSQAVLDAMGSCLT
                     NKYAEGYPGRRYYGGCEVVDKVEQLAIDRLKELFGAEYANVQPHSGSQANAAVYLSLL
                     QPGDPILGFNLSHGGHLTHGSPVNYSGKTFQAHFYGVEEATGLIDMDKVEAIALETKP
                     KLIICGASAYSRDWDYARFRAIADRVGALLMGDIAHPAGLIAAKLLNNPLEYCHIATS
                     TTHKTLRGPRGGIIMMGKNFDNPWGRTTAKGTLIKMSAILDSGVFPGMQGGPLEHVIA
                     AKATAFYEALQPGFKTYAAQVIENAQAMASAFVEKGYKVISGGTDNHLMLIDLRSKGA
                     TGKAAEEALGKADITVNKNMVPFDTEKPMITSGIRIGTAAVTSRGLKAADCARVAGWI
                     DSIITHSDNEEHIGAVRAEINQFMQQFPLYPGVA"
BASE COUNT         1661 a         1889 c         1826 g         1739 t
ORIGIN      
        1 tggcctatga attttgtaag tggtgttcag gcagttaact ccttccacac cgagcgggct
       61 ttggccaacg ctttgccgtc ggaagcgcgt accaagcggt gcatacgaat gttttcctgc
      121 tctgtcgccg tttccgaaat gacttcttcg cccagcgtac attctgccag ataatgcact
      181 tccatatccg ataaagctgc atcgcgcaag aggtcttcat cggcagaagc cagcagccat
      241 tccacatatt tactgttggt gagatggaaa ttaaaatcga gatcgaacca gtgaatggtg
      301 aatcggatgg cgttggaggc cgttccaaat tcgggcagga cttcagcggc acgcgccaga
      361 tgcgagtcag aggccggtag taattcattg taagccagca tatcagccgg aatgcgggcc
      421 atgcggcgcg ttgcagtttg catcagcaac caggtagtgg aggcttgtgc aatttcctgc
      481 ccggcagcgt cgtacatccg gtaatcccga tgcgtaaaaa cccgttcaaa ggcagaggga
      541 tgtgtaataa tttctacggt ttcgcccaaa cggggcaggc gctcgaaatg caggcgttgc
      601 cgcatcagca cccaggagag gccgcgaggc tccaaatccc acacggagag ttgcaagcgc
      661 attacatgtt ccatcgaagc ctcctgcatg atgcgcacca aggcgggtgc agaggctatt
      721 ttgtgcgggt ctatctcgta acttctgatg acgaaggccg agcgatgaac caaatcagca
      781 gccgtgaacg cgttcattgt ggaatcagcg gggcgctttt tccaagttgt tcatcaccaa
      841 ttcggtagca tccatcagcg tgcgccaatc tttgcgtatc tggtcttcct tggctttggc
      901 ggcaaccgtc ttgccggatt tattgagttg ttctaatgcc acgacttcct gctccaattc
      961 caaacggcgg cgttccaact cttccaactt ggccagcgag gcatcgtcgt cagaagattt
     1021 ggccttgctc ttgagcgatc ccaacagacc tcccacttta tccactgctt cgtcgtactt
     1081 gccttcatcg aatttttgct tctccgactt cagcaagccc caggttgcct tccccagatc
     1141 gcgaacatcg gtgaagattt ctttggaagt ggctttttct tccggcgtac cgaaaaagta
     1201 gttgtaaatc aagacgccta caactacgac ggcgactaat ttgagcaggc ttttcatgat
     1261 gatgagttat tttaaaacaa gaatcaatcc cggatatacg gataatccgc ctgaacgtaa
     1321 ttgtcttcgt acaaagtaga accggccgga tattcagagt tttcagcaaa ctccacacag
     1381 tcgtcaattt ctgccttgac gcggtcccga atagcctgta tttcttcctc tgtggcaatg
     1441 ccgttttcca atatcttgct ctcgatgagc ttgatcggat cgcgttcttt gtacgattcc
     1501 acttcctctt tggtgcggta cttggccggg tcggaaaccg agtggccttt gtagcgatag
     1561 gtacgaattt cgaggaaata cgggccgttg ccggcgcgga tgtgctcggc ggcacgggta
     1621 actgcctcat gcacagcttc gggattcatg ccgtcaacgg cttcgctggg catatcataa
     1681 gaagcgccca gtttgtacaa ttccaccacg ttgctggtgc gacccaccga tgtgcccatc
     1741 gcgtagccgt tgttttccac tacgtacagc acaggtattt tccaggccat agccatgttg
     1801 aaagactcgt ggagggcgcc ctgacgggat gctccgtcgc cgaacagcgt cacgcagagg
     1861 ttgtcggtac ccttgtattt ttcagcaaac gcaatgccgg tgccgatggg aatctgcgct
     1921 cctacaatgc cgttgccgcc ataataattg agcggcttgg agaaaaagtg catcgaaccg
     1981 ccttttcctt tgacgatgcc ggtctctttg ccgaacagtt cagccattgc ctcgcgcgag
     2041 ctgacgccgc gccccaatgc cgtgccgtgc tggcgataag ccgtcacgat gctgtcttcg
     2101 cgnnnnnnnn nnnnnnnnnn nnnnnnnnaa tggccgattc gatcccggca gccaccgcct
     2161 cctgaccgat gtaaacgtgg caaaaaccac gaattttttg ctggccgtac atcaccaatg
     2221 cccgctcttc gaagcgacgg atgcgcagca tgagttcata ccaatacatg tagcggtctt
     2281 ttgggtgttg cataccgcct gttgctttct tttttctggc taccatgata ttatcgttag
     2341 agtcaattca ggtgaaagat cgttttgagc gcgcaaaaat aacaatttgg ggaataaaca
     2401 ccacaatgac acactggttc ggttatactt ctttgaccaa aaaaaagcca tcgctaccct
     2461 gcgcctgata caaaggcatg agaatgagcg agtcttcttt ggctttaata gggccaaagc
     2521 ggtcatgcgc caatatctca tcttgtttta ccgacttgaa attgtgataa tcaggagtca
     2581 tcctaaattc atcctgaggt tgaatatgat gcacatacct caacgcggcc actttgggaa
     2641 ggcctttgga ataagcaatc agcagatcat cgtggcgatt ttccacgtcg tcggcatcca
     2701 cgcaaccaat ggtgcgcata caattgatga tggccgcaat acagcgattg actgacagcg
     2761 ggtcgtcgtg ctgaccggct tcaaaaccta aagccactac ttccggcgcc tgtatgtttt
     2821 ctttattgaa atagtgcagc atcgttccct gaagccccgc caacatgccg gttataaccg
     2881 gcgcatgcag ttcctgcgcg atgcgaatgc tttcaggatc atctgtggca atgccgaaaa
     2941 tgccgccgtc tgcggtggtg gtgtgcaagt cgagcacgat cactttttcg gggcggtaca
     3001 tggcgatttc ccgctcaacg gcttcggcta attctctcag ttcgcgttct tcgtagcgca
     3061 aatcggccgg atgcgtatgc ttcagtctgt tgatcaaatc ggggcgccac atgcggttga
     3121 ggtcttgagt ataaaatcgc tttcgacggc tcagcgccnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnngga ttaacggcag tctcgcgttc caacaattta
     3241 aaaacagttt ccaatgctct gaccccggca agttcgttgc cgtgaatgcc gccaaaacaa
     3301 atcaacaagg ggcctcgatc agggccggta tatcttccga taattcgttt catttcgtag
     3361 tgtttctggg ccaacgccaa agaaatttgc aggcgtaggt tcggtaggga cgccaccgtt
     3421 cggcagcttc taccattttt ttatgcaatg ctttgccgct ttcctgccat ccaaacaatt
     3481 gagccataga ttgttgtatg ccgaggtcat ctaagggcaa cacgtctgta cgattgaggg
     3541 taaacatgag taccatttct gccgtccagc ggcctacgcc tttgatttgg gtgagaaaac
     3601 gaatgatgtc ctcatcgctc atttccgacc agtttttacc gcgatgctcc ggcgaagaga
     3661 aaaactcggc cacattgcgg atgtagtgcg ttttggaatt ggacaggccc accgcccgca
     3721 actgctcgaa ctccattgcc aaaacatctt ccggctcagg atattgattg ctgaacaaag
     3781 ccataaaccg tccggagatg acctgcgcta ccttgcccga caactgctgc gagatgatgg
     3841 acaccagcaa atcataatac acgtcgctgt tgccctgccg gaaatcgggc ttgtctatgg
     3901 tttcccgaat gacgcgggcc atggccggat cggcagagag gtgggaaaga atcaagtcgc
     3961 tcatgggggc aaaggtatga attattagca aagcgacggg cgnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ctgtgggccg ggcggctggg
     4141 cgggatattc caatcgcgcc atttcctgtt tttgagcatt gacgagcaag agcgtcacgt
     4201 cggcctgctg agcgatttcg tagtcaattt ttacttttcc cgaggccgga ttgggtacca
     4261 agcgaggcag ggcatcccag ttgccgccca gatcgtcgga ttcagcgggc gcgggagcag
     4321 tcgtatgctg agggcgttct ttcgtcgaag ccgactccgc taccggaggc tttttcttat
     4381 aaatgcggat gacatcaccg ccgggcggac tccaaagcga gaggccaaga ggcggttcaa
     4441 agatgttgtt caacgatacg gttcccgcct gcgctgcatt gtcggtcatc accgagcgta
     4501 cctcgatttt ttcctgatct ataaaaatcc agttgaagtg attgaaactg ccgctggccc
     4561 gcgtccagga tttatcatcg tcgttttccc gcagcggagc gccccaacag ccttcgccga
     4621 caaatacggt accggttgca tcgtcccgaa tgaagcccat atcgcttccg ggaccggtat
     4681 cagggcgcaa gggataagtc catttggcca catgcgagtc ggactcaatg gccaatttca
     4741 gcccgtgctc gtgaaacaac tcggcccaag ccaagtaaat atcatctttt tcagttttgg
     4801 cggctgtatg cgggcggatg gcgtggtggt attgggccat tttccagact acggaattcc
     4861 cggttgtttt caggtcgttt tccaaccagt ttttctgctc gccgcctgcc ggaatcaagg
     4921 tgttgagggt ataaatgcga accagattgc cgccgaaatt gaaagcataa aaattgccgg
     4981 gatgcgccag atcgaacatg tcaatcaggg ttttattttc cgattcgtga ttgccccttg
     5041 ctaccagaac aggcgtgatg tgcccgtcgg cggcgatggt cagttgccag tctttgagcc
     5101 agtcttccca ttcggtagat gaatcgttga cagtcatatc tccattgaaa agaacgaagt
     5161 gcggacgcaa tttggccacc attgcattgg cgcttttgcg ggcttcgcgg tggttgcgcg
     5221 agtcgccgcc ggcaatgatg gaaagccttt gcgtaggctg gtcgggagcg gtgcggaaag
     5281 aatagcgcgc cgtagagcct tcagaatcct taataataaa atagtaagca gtattgggct
     5341 gcaaaccgct aagccgaacg aaatgattgt tcatgccctt agcaaagatc actctgtcgg
     5401 gttttctcga aagcttgtaa gcgttgaaat tgcgcccctg atccacgacg tcgaaataga
     5461 ctaccggatt ggcgccctgc acctgatccc aggcaatgac catagaggtg gcgggattat
     5521 cgcgccaggt gcaacgaatc ttatcactca atgcgttgag gctcagttgg gaagcaagtc
     5581 cgaaaagaag tatcagagcg atgtgcagat ttgtttttct catattcacg tttgtcttgg
     5641 tcgggcaatg gtacaaatta aggtcaatct tttttcattt attgtacctt tggccgaaaa
     5701 tttaagattc aaattcagac caaccaacat gacgaaagac acagcgctct ttgatctcat
     5761 tcacgaggaa ttgacccgcc aacgcaaagg catcgaactc atcgcttcgg aaaactttac
     5821 ctctcaggcc gttttggacg ccatggggag ttgtctcacc aacaaatacg ccgagggcta
     5881 ccccggtcgc cgctactacg gcggctgcga ggtggtggac aaagtagaac aattggccat
     5941 agaccgcctc aaggaactct tcggcgccga atacgccaac gtgcagccac actccggctc
     6001 gcaggccaat gccgccgttt acctctcgct gctgcaaccc ggcgatccca ttctcggctt
     6061 caacctctcg catggcggcc acctcacgca cggttcgccg gtcaactatt cgggcaaaac
     6121 atttcaggcg cacttctacg gcgtagagga agctaccggc ctgattgaca tggacaaagt
     6181 ggaagctatt gcgctggaaa ccaagcccaa actcatcatc tgcggcgcat cggcctattc
     6241 ccgcgactgg gattacgccc gcttccgcgc cattgccgac cgcgtgggcg ctctgctcat
     6301 gggagacatt gcacaccctg ccggcctcat tgctgccaaa cttctcaaca atccgctcga
     6361 gtactgccac atcgccactt ctaccacgca caaaaccctg cgcggtccca gaggcggcat
     6421 cattatgatg ggcaaaaatt tcgacaatcc ctggggacgc accacggcca aaggcacgct
     6481 catcaaaatg tcggccatcc tcgacagcgg ggtttttcca ggtatgcagg gcggcccgct
     6541 cgagcatgtc attgccgcca aggccaccgc tttttacgaa gccttgcagc cgggattcaa
     6601 aacgtatgct gctcaggtga ttgaaaatgc gcaggcgatg gcttcggctt ttgtagaaaa
     6661 aggttataaa gttatctccg gaggcaccga caaccacctg atgctcattg atttgcgctc
     6721 caaaggcgcc accgggaaag cagccgaaga agcgctgggc aaagccgaca tcacggtgaa
     6781 caaaaacatg gtgcctttcg atacggaaaa accaatgatt acttccggca ttcgcatcgg
     6841 cacggcggcc gtcacctcgc gcggactgaa agcggccgat tgcgcccgcg tcgccggctg
     6901 gattgacagc atcatcactc attccgacaa cgaagaacac atcggggctg tgcgcgccga
     6961 gatcaatcag tttatgcaac aatttccgtt gtatccggga gtagcgtaaa gctgaggaaa
     7021 agaaaacagg cagttccgca atattgtact gtaacgcttc tgagtttcgt gagtgtaaga
     7081 ccggaactgc ctgtacctaa acgcctatat catcaaactg gtactattac gattgaagtg
     7141 gataattcgc tgcttagggc tatagttttt ttaactctgt gatgatgaca ggtttctgca
     7201 taaagaattt aatgacaccc ttcactctct ttgctttcat cttcattggc agtttttttt
     7261 tcagcaattg gacagccaac ggaagtacga cagaggagag ataaaat
//
LOCUS       DAORLF010000236         7300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5516_length_7300_cov_2.032436, whole genome shotgun sequence.
ACCESSION   DAORLF010000236 DAORLF010000000
VERSION     DAORLF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_7300_cov_2.032436"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1347
                     /locus_tag="PK198_16995"
     CDS             145..1347
                     /locus_tag="PK198_16995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF40493.1"
                     /translation="MQKGYSLLGFLVLLLTTVSFTRFQAGNKPRIAEEEMTAPVLDEA
                     TSQLVNQYDAFVRESLQREQFPGTAVVIVKDGRVIFKQCYGVRTANTTDSVNVNTLFR
                     LASLSKGFASVLAGMLVQDGYFEWDDPVKQYVSDFSMYRQSYTDSLSVRHVLSHTTGL
                     PRQTFSNLIERDESYASARYRLREVKPTHPLGKYYNYQNVTYSLIGDIAEQTTGKRFE
                     ELLYQRIFLPLRMTNAGAGYNNFLLDTFNVAWPHRIENEQYKRVKVLPNYYEVMPAAG
                     VNASISDMGEWLRFLLGHRPDVMNKEALNELFNKQVSVPLAEGNHRAYPESLEAWYGL
                     GWRGIVSNERRVIFHGGYVNGFRSEIAFIPSEDIGIAILSNSPSWFINSSVPRFLQMY
                     WGLPYEQN"
     gene            1355..2224
                     /gene="rfbD"
                     /locus_tag="PK198_17000"
     CDS             1355..2224
                     /gene="rfbD"
                     /locus_tag="PK198_17000"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRF40494.1"
                     /translation="MTGAVGQIGMELAAVAPQFPDFDFVFADSQQLNISSHDDIQRMF
                     ASRKFDYCINAAAYTAVDKAQTERKKTALANMIGPYLLACACKENQTRLIQLSTDYVY
                     HNGLNRPLLEDDPVEPKSAYAFSKCAGDEAVLRFHSEGAMVVRTSWVFSSFGHNFVKT
                     MLRLGRERPELRVVFDQIGTPTYARHLARALLTAIASAESGAVNRDLLRGVYHFSNEG
                     VASWYDFALAVFEMSSLPCAVLPIETKDYPTPAQRPPYSVLNKTKWKTAFGQAIPHWR
                     EGLRECLEILDRS"
     gene            complement(2221..2931)
                     /locus_tag="PK198_17005"
     CDS             complement(2221..2931)
                     /locus_tag="PK198_17005"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF40495.1"
                     /translation="MIKWLFVIVAAGAVLWGLWKYGRSLLTPLRPLKRSVETVDSVLH
                     KVQPGAMSRLKPYLERAGFEELPSELVLVGLKEERQLEVYARKAGNFVLLKSYPFTAF
                     SGRLGPKLKEGDRQIPEGIYKIEYLNPNSAYYLSMKVSYPNAFDLQKSRFTDPKELGG
                     DIFIHGKAVTIGCIPVGDPAIEELFLLVGKAMEREVKVIISPRDFRVNAEFPEIEGID
                     WEHELYENVRKELALLKR"
     gene            complement(2928..5750)
                     /locus_tag="PK198_17010"
     CDS             complement(2928..5750)
                     /locus_tag="PK198_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF40496.1"
                     /translation="MKTAFRLFFLLFLCPFVADAQVYTDPYFPRADQPVTIYFDATQG
                     TGGLANCNCNVYLHTGLITSASTSPSDWKHVVTTWGSANAAWQMTPVPGQPNLYSFTI
                     TPSIRTYYGAPITTTILKMAFVFRNASGTLQGKGPGGSDIFYDVYPANLPFTGLLLEP
                     AERSIVTTAGQNISLRYVTSESSNIQLLDNGNLVTQVTGTELDWTLTASSGEHLIEVV
                     ADNGTTEIREAFGYAVFESQPALDPPAGTRPGITYMGDTAVRLRLYAPNKQYAFVVGD
                     FNGWAPRQMHRSADGNTYWIDVTGLHPGSFYAFQYIVSGNIRIADPHSTLVLDPAHDP
                     WISASTWPGLPAYPTGRASGITSLIQPGAPQYDWQTENFQRPAKSKLVIYELLIRDFL
                     GTRNYNTLIDTLDYLDRLGVNVIELMPVNEFEGNSSWGYNPSFHGALDKYYGTPNDFK
                     RFVDACHARGIAVVLDVVYNHAFGQSPLSQLYWDAANNRPAANNPWLNPIARHPFNVG
                     YDFNHAAQATKDYITQVMQYWLTEYRIDGFRFDLSKGFTQTNSGSNVSTWSAYDASRI
                     TILKYYADRVWETTPGAYVILEHFADNTEERELANYGMMLWGNLNHEYNEASMGFTSN
                     FSWASHLARGWNQPHVIAYAESHDEERLMYKNLQFGNMSANHNARQLRTSLRRMEMTS
                     AFLYAIPGPKMLWQFGEVGYDFSIFTCENGTVQSGNDGCKLSPKPVRWDYYNNADRRR
                     LFDVTSALIHLKKNYDAFSTNNFSLNVANPNKTIHLLHPTMNVAVQGNFDVQSRNIVN
                     PFPNIGWWYEYFTGDSLLVASTLTPLSFYPGEYRLYTTVKLPPPPTGYVTSASEVVQN
                     VFGLTLMPNPSPGGWGQAFFAIERGGEAAIVVSDMLGRTVKTLYGGRLEAGEHQFELP
                     QGLPAGAYLVRLQVGHQIEVQKWMVQ"
     gene            complement(6006..>6434)
                     /locus_tag="PK198_17015"
     CDS             complement(6006..>6434)
                     /locus_tag="PK198_17015"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HRF40497.1"
                     /translation="IYTRLISAIEMKQIAEPYIRRRANRHLEKGRVVIFASGTGSPYF
                     TTDSAAALRANEINADVILKGTRVDGIYTADPEKDPNAVKFDTISFAKVISLGLSVMD
                     MTAFTLCQENNRPIIVFDINDPDNLRRIVLGEQVGTLVEG"
     assembly_gap    6435..6496
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6497..6733)
                     /gene="pyrH"
                     /locus_tag="PK198_17020"
     CDS             complement(<6497..6733)
                     /gene="pyrH"
                     /locus_tag="PK198_17020"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRF40498.1"
                     /translation="MTLKYKRILLKLSGESLMGDQKFGISADMLKHYAQQIKILVDLG
                     VEVAVVIGGGNIFRGLQAAASGIDRVQGDYMGMMA"
     gene            complement(6811..>7300)
                     /gene="tsf"
                     /locus_tag="PK198_17025"
     CDS             complement(6811..>7300)
                     /gene="tsf"
                     /locus_tag="PK198_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRF40499.1"
                     /translation="ENITVGDKVTEQLGVIGEKIELAAYERLEAEMVAPYIHMGNKAG
                     VLVGFSKAAPDHFEALRSVAMQVAAMKPVAVDRDDVPQSVIDKEIEIRTDIALNDPRN
                     AGKPRELLEKIAQGSLNKFFQEVTLINQDFVKDGSKKVGEYVKSLDKDLAVTAFKHVT
                     LG"
BASE COUNT         1695 a         1917 c         1901 g         1725 t
ORIGIN      
        1 cgagatggaa tctcgcgcga gcggggcggg gcgtttacga ctgtcttgca aggttcggtt
       61 cataaattac taccccgcgc tgatggtttt tccaaaaacc ttttatcttt gcttccctct
      121 gtgaaacagc cacaccctta ctttatgcaa aaaggatatt cgttgctcgg ctttttggta
      181 ttattactga ctaccgtatc gttcactcga ttccaggcag gcaacaagcc gcgcattgcc
      241 gaagaagaga tgacggctcc ggtattggat gaagctactt cccaattggt caaccaatac
      301 gatgcttttg tgcgcgaaag ccttcagcgc gagcagtttc ccggcacggc agttgtcatt
      361 gtcaaagacg gcagagtcat ttttaaacaa tgctatggcg tgcgaacagc caacactacc
      421 gactccgtca atgtgaatac cttgttccgg ctggcttcat tatctaaagg atttgcctcc
      481 gtgttggccg gaatgctggt gcaggacggc tattttgaat gggacgaccc cgtgaaacaa
      541 tatgtgtccg atttcagcat gtatcgccaa agctataccg attcgctttc tgtgcgccat
      601 gtgctgagcc ataccaccgg actgccccgc caaaccttct ccaacctcat tgagcgcgac
      661 gaatcttatg cctccgcgcg ctaccggctg cgagaggtaa aacctacgca cccgctgggc
      721 aaatattaca attaccaaaa cgtcacttat agccttattg gcgatattgc agaacagacc
      781 acaggtaaac gctttgaaga attgctgtac cagcgcattt tcctgcccct tcgcatgacc
      841 aatgccggag cgggctacaa caatttcctc ctcgatactt ttaatgtagc ctggccgcat
      901 cgcattgaaa acgagcaata taagcgcgta aaggtgttgc ccaattatta cgaagtgatg
      961 cctgccgccg gcgtcaatgc cagtatttcc gacatgggag agtggctgcg tttcctgctg
     1021 gggcatcgcc cggatgttat gaacaaagag gcgctcaacg aattgttcaa caaacaggtg
     1081 agcgtgccgc tcgccgaagg caatcaccgc gcctacccgg agtccctcga agcctggtat
     1141 gggctgggct ggcggggcat tgtgagcaac gaacgccggg tcatctttca cggcggctac
     1201 gtcaacggct tccgctccga aattgcgttt atcccctctg aagacatcgg catagccata
     1261 ctcagcaatt cgccctcgtg gttcatcaac tccagcgtgc cgcgtttttt acaaatgtac
     1321 tggggcctgc cgtatgagca aaattagcat tctcgtgacg ggcgcagtcg gacagattgg
     1381 gatggaactg gcagccgtcg cgccgcaatt ccccgatttc gacttcgttt ttgccgactc
     1441 tcaacagttg aatatctctt cacacgacga tatacaacgc atgtttgcat cgcgcaagtt
     1501 tgattattgc atcaacgcag cagcttacac agcagtggac aaggcccaaa ccgaacggaa
     1561 aaaaactgcc ttggcgaata tgataggccc atacttgctg gcttgcgcct gcaaggaaaa
     1621 ccaaaccagg ctcattcaac tttcaacaga ttatgtttat cacaatggac tgaaccgccc
     1681 tttgctggag gatgatccgg tagagccgaa aagtgcttac gctttcagca aatgtgccgg
     1741 cgatgaggcg gtgctgcgtt ttcactccga aggcgcgatg gtggtacgca cttcctgggt
     1801 gttttcgtct ttcggccaca attttgtcaa aaccatgctc cgcttgggcc gggaacgccc
     1861 cgaattgcgc gtcgtcttcg atcagatcgg cacgcctact tatgcccggc atttagcccg
     1921 ggcacttttg actgccatcg cctctgcgga gtcaggagcc gtaaaccggg atttattgcg
     1981 gggcgtttac cacttcagca atgagggtgt ggcgagctgg tacgatttcg ctttggccgt
     2041 gtttgaaatg agcagtctgc cctgcgccgt gttgcccatc gaaaccaaag attaccccac
     2101 gccggctcaa cgcccgccgt acagcgtcct gaacaaaacg aaatggaaaa cagctttcgg
     2161 acaggcaatt ccgcattggc gggaagggct gcgggagtgt ttggagatat tggacaggag
     2221 ttagcgtttc aacaacgcca actccttccg cacattttca tacaattcat gttcccagtc
     2281 aatgccctcg atttcaggaa attcagcgtt caccctgaag tctctggggc tgatgatgac
     2341 tttcacctcc cgttccatcg cttttcccac cagcaaaaac aactcctcta tggccgggtc
     2401 gcccaccggt atgcagccga tggtgaccgc cttgccatgt ataaaaatat ctccgcccag
     2461 ttctttggga tcggtgaagc ggctcttttg caggtcgaaa gcattgggat agctgacttt
     2521 catagacaaa tagtaggcgc tgttggggtt cagatattct attttgtaaa tgccttccgg
     2581 gatttgccgg tcgccctctt tgagcttcgg ccccagccgg ccgctgaacg ccgtaaatgg
     2641 ataggatttc agcagtacaa aattaccggc tttgcgggca tagacctcca attgccgctc
     2701 ctcctttaat ccgaccaaga ccagttcaga cggcaattcc tcaaaacctg cccgttccaa
     2761 atacggtttc agcctcgaca tagcgccggg ctgtaccttg tgcagcaccg agtccactgt
     2821 ttcgacactg cgtttcaggg gccgcaatgg cgtcaacaga ctcctgccgt atttccaaag
     2881 cccccacaaa acagcccctg ccgccacgat gacgaacagc cattttatca ctgcaccatc
     2941 cacttctgca cttctatctg gtggcccacc tgcaagcgca ccaaataagc gcccgccggc
     3001 aagccctgcg gaagctcaaa ctggtgttcg cctgcttcaa gccgcccgcc atacaaggtt
     3061 ttgacggtcc tgcccaacat atccgacacg acaatagctg cctctccgcc ccgctcgatg
     3121 gcgaagaatg cctgccccca gccacccgga ctcggattgg gcatcaaggt aaggccgaag
     3181 acattttgca ccacttccga cgccgaggtc acataacccg ttggcggcgg cggcaacttc
     3241 accgtagtat aaagacggta ctcgccgggg taaaaactga gtggcgtcag ggtgctggct
     3301 accaagaggc tgtcacctgt aaaatactca taccaccagc cgatgttggg aaatgggttg
     3361 acaatattgc gcgattgcac gtcaaaatta ccttgcaccg ccacgttcat cgtcgggtgc
     3421 aggaggtgga tggtcttatt gggattggcg acattgaggc tgaaattgtt ggtactgaag
     3481 gcatcatagt tctttttcag atgaatgagc gcgctggtta cgtcaaaaag gcggcggcgg
     3541 tctgcgttgt tgtagtaatc ccaacgaacg ggtttgggcg agagtttgca gccgtcgttg
     3601 cccgattgta ccgtgccgtt ttcacaggta aagatggaaa aatcataccc tacttcgccg
     3661 aattgccaca gcattttggg gccgggtatg gcatacagga aggcagatgt catttccatc
     3721 cgacgcaggg aggtgcgcaa ctgacgtgca ttgtgattgg ccgacatatt gccgaattgc
     3781 aggtttttat acatcaatcg ctcctcgtcg tggctttccg cgtaagcgat gacgtgcggc
     3841 tgattccagc cccgcgccag gtgggaggcc caactgaaat tggaagtaaa acccatgctg
     3901 gcctcgttgt actcatggtt caggttaccc caaagcatca tgccgtagtt ggccaattcg
     3961 cgctcctccg tgttgtcggc aaagtgctcc agtatgacat aagcccccgg cgtggtttcc
     4021 cacacgcgat ctgcatagta tttcagaatc gtgattcggg aagcatcata agcgctccag
     4081 gtgctcacgt tgctgccgct gttggtctgg gtaaaccctt tggacaagtc gaaacggaag
     4141 ccgtcgatgc ggtactcggt cagccagtat tgcatcacct gcgtgatgta gtctttcgtg
     4201 gcctgcgccg cgtggttgaa gtcgtagccc acgttgaacg ggtgccgcgc tatgggattc
     4261 agccagggat tgttggctgc gggacggttg ttggctgcgt cccaatataa ttgcgaaagc
     4321 gggctttggc cgaaagcatg gttgtacacc acatccaaaa cgaccgcgat gccgcgtgca
     4381 tgacaggcgt ccacaaaacg cttgaaatca ttgggtgtgc cataatactt atccaaggcg
     4441 ccatggaacg acgggttgta gccccaggag ctgttgcctt caaattcatt gaccggcatc
     4501 aactcgatga cgttcacgcc gaggcgatcc aaatagtcta aggtgtcaat cagcgtattg
     4561 taattgcgcg tgccgaggaa atcgcggata agcaactcgt atataaccaa tttcgacttg
     4621 gccgggcgct gaaaattctc tgtttgccaa tcgtattgcg gtgcgccggg ctgtatcagc
     4681 gaggtaatgc ccgatgctct gccggtggga taggccggca atccgggcca ggtagaagct
     4741 gaaatccagg ggtcgtgcgc cggatcgagt accaatgtgc tgtgtgggtc ggcaatgcgg
     4801 atattgccgc tgacgatgta ctgaaaagca taaaaactgc cgggatgaag cccagtgaca
     4861 tctatccaat aggtattacc gtcagcgctg cggtgcattt ggcggggcgc ccagccgtta
     4921 aaatcgccta ctacaaaggc atattgttta ttgggcgcgt acaatcgcaa tcgaacggcc
     4981 gtatcgccca tgtaggtaat gccggggcgg gtgccggcag gaggatcaag agccggctgc
     5041 gattcaaaca ctgcataacc aaaggcttcc cgtatctccg ttgtaccatt gtcagccact
     5101 acttcaatca agtgctcgcc ggagcttgcc gtcagcgtcc agtccaattc tgtgccggtc
     5161 acctgcgtta cgaggttgcc gttgtccaac aactgaatat tagacgactc tgaagttaca
     5221 tagcgcaacg aaatattctg tccggctgtc gtgacgatgc tgcgttcggc cggctccaac
     5281 aacaaacccg tgaaaggcag gttggccgga taaacatcgt aaaaaatatc ggagccaccg
     5341 gggcctttgc cctgcaatgt gccgcttgcg tttctgaaca cgaaggccat tttcagaatg
     5401 gtcgtggtaa tgggcgctcc ataatatgtt cgtatagagg gtgtaatggt aaaactgtac
     5461 aaattgggct gccccggcac aggggtcatt tgccaggcag cattggccga cccccaagtc
     5521 gttaccacat gtttccagtc gctgggcgaa gtacttgctg aagtgatgag gccggtgtgc
     5581 agatagacgt tgcagttgca attggccaaa ccgccggtac cctgcgtggc atcaaaatag
     5641 atggtcacag gctgatcggc tcgcgggaaa taaggatcgg tataaacctg agcatcggcc
     5701 acaaacgggc acaaaaagag aaggaaaaac agacgaaaag cggttttcat ggtctttgta
     5761 ttgctgggtt ttggcccgat atagggcggc ggataacccg tttcgggagg cgaagttaga
     5821 taaaatctct cttacctcac attgttacca cacataatga atggggctgt tttagcaaga
     5881 taaattgcaa aacatgccaa aacgattctc ccctctcagt tggcaagatg aactgcgaag
     5941 cagattactt ataataaacc gccgaaggca gattacagcg aagcagatga gcccccaaag
     6001 gcagattacc cttccactaa tgttcccacc tgctcgccca gtacgatgcg gcgcaaatta
     6061 tcagggtcgt tgatgtcgaa aacgatgata ggccggttgt tttcctggca caaagtaaag
     6121 gccgtcatgt ccatcacgct gaggcccaga ctgatgactt tagcaaagct gatggtgtcg
     6181 aatttaacag cgttggggtc tttttcgggg tcggctgtgt aaatgccatc cacgcgggta
     6241 cctttgagaa tgacgtctgc gttgatctca ttggcgcgca gtgcagccgc cgaatcggta
     6301 gtaaaataag ggctgccggt gccggaagca aaaataacga cccggccttt ttccaaatgg
     6361 cgattggctc tgcggcggat gtacggctcg gcaatctgct tcatttcaat agcagaaatg
     6421 aggcgggtat aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnagcc atcatgccca tgtagtcgcc ctgcacccgg tcaatgccgg
     6541 aagccgccgc ttgcaagcct ctgaaaatat tgccgccgcc aatcacgacc gccacttcca
     6601 cgcccagatc caccaaaatt ttgatctgct gagcgtaatg tttgagcata tcggcggaaa
     6661 tgccgaattt ttgatctccc atgagcgatt cgccgctgag cttgaggagg atgcgcttgt
     6721 atttcaaggt cataagaaag gaataggaaa ataaaggtca aaaaaaaggc gaattagaag
     6781 tctaattcgc ctttcggaac aaaattggta tcagccgagg gtgacgtgtt tgaacgccgt
     6841 cacggccagg tctttatcta aactcttgac gtactcgccc acttttttgg agccgtcctt
     6901 cacgaagtcc tgattgatga gggtaacttc ctggaagaac ttgttgaggc tgccctgagc
     6961 gattttttcg agcagttcgc gcggtttgcc ggcgttgcgc gggtcgttga gcgcaatgtc
     7021 ggtgcggatt tcgatttctt tgtcaatcac agattgcgga acatcgtcgc ggtcaacagc
     7081 taccggtttc atagcagcta cctgcatggc cactgagcgc aatgcctcaa agtgatcggg
     7141 agcggctttg ctgaagccca ccaaaacacc ggctttgttg cccatgtgga tataaggcgc
     7201 taccatttcg gcttcgaggc gctcgtaagc agccaactcg attttttcgc cgataacgcc
     7261 gagttgctcg gtcactttgt cgcctacggt gatgttttcg
//
LOCUS       DAORLF010000237         7290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5525_length_7290_cov_1.808708, whole genome shotgun sequence.
ACCESSION   DAORLF010000237 DAORLF010000000
VERSION     DAORLF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_7290_cov_1.808708"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK198_17030"
     CDS             complement(<1..514)
                     /locus_tag="PK198_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF40500.1"
                     /translation="MKRYFNTTGLCSPKDHYMVNPFRDIYDDILRLIEAKQYFLIHAP
                     RQTGKTTFLHSLAHRLNREGKYVATVCSLESAGYQSISVEDANHVFIQSMYNRASLFV
                     DESNMPPHPDNYSKSSVLLQRYLTEWCNRLEKPLVLLLDEVDALYDDVLISTLRQLRD
                     GFQSRPSGFPQ"
     gene            complement(547..>1832)
                     /gene="glmM"
                     /locus_tag="PK198_17035"
     CDS             complement(547..>1832)
                     /gene="glmM"
                     /locus_tag="PK198_17035"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRF40501.1"
                     /translation="AAYGAWLLQTGAAPKIVLGRDGRISGPMVGALVTQTLLALGIEV
                     IDLGLSTTPTVEMAVPREQAGGGIILTASHNPAHWNALKLLNASGEFISQADGEAFLA
                     LLQNGGITYAEVDRLGACRSDDTALDYHVACILALPDVEVEAVQNRRFRVVVDAVNST
                     GAIAMPAILEALGCEVYILNGEVNGRFAHNPEPLPEHLGGLSEAVRNLGADLGIAVDP
                     DVDRLALVCEDGSFFGEEYTLVAVADYILSRRKGNTVSNLSSSRALRDVTQQHGGQYF
                     AAAVGEVNVVQKMKAVQAVIGGEGNGGIIVPELHYGRDALAGAASFLSHLALSGNSIS
                     QLRRRYPDYFMIKDKISLQPDTDMNALLSQLHEQFRHESCDVSDGLKIDFADGWVHLR
                     RSNTEPIIRIYAESVSEQRAQELAERMKGAALGMS"
     assembly_gap    1833..2040
                     /estimated_length=208
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2085..2531)
                     /locus_tag="PK198_17040"
     CDS             complement(2085..2531)
                     /locus_tag="PK198_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40502.1"
                     /translation="MFSRINPSHPVVMAALCLGGVLVVNLLAKLLQISGMQVTPRFPW
                     MAAAAFLLLFSIFNSVASLFAADGVKYWGRSIYSFLGLALGSGLMAYAFSSLSIGEAG
                     SYRWIFVVLTFGYLVFISIMNIARRIVEFAQREEWSHPRIRQKKRR"
     gene            2761..3498
                     /gene="lipB"
                     /locus_tag="PK198_17045"
     CDS             2761..3498
                     /gene="lipB"
                     /locus_tag="PK198_17045"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRF40503.1"
                     /translation="MPKVTYRNLGRISYREAWSLQEALLRELVDRKLANRTRTAEGLP
                     AEEQQHYLLFCEHAPVYTLGKSGSEDNLLLDAETLEKEGFEFFKINRGGDITYHGPGQ
                     IVGYPIFDLDCFFTDVHKYVRYLEEAVIRTLAEYGIAAGREQGYTGVWLEGDGTWLLS
                     SELGDRKIGAIGVRISRWVTMHGFAFNVNTDLGHFKNIVPCGIADADKDVTSMQRELG
                     RVMDMEELREKLRGHFAQLFEFEWKTI"
     gene            3495..3908
                     /locus_tag="PK198_17050"
     CDS             3495..3908
                     /locus_tag="PK198_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40504.1"
                     /translation="MKKDILIHKVEDLAIAIVPRDESLEDKEELWDVFLINLKEEPIT
                     CVLVNSTGYSEQQGGEQRRTTTLRHFFEEVGPLSVVQVEPIQVQVFDLTNEYWVSFLH
                     DNYMYDKKYVFVRGSIRPEHFMMVPFINRKGVVIR"
     gene            4058..4582
                     /locus_tag="PK198_17055"
     CDS             4058..4582
                     /locus_tag="PK198_17055"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRF40505.1"
                     /translation="MKAKKVSESNTVMTEMVMPNDTNPMGNLMGGNLLRWMDIACGIC
                     AGKHCEAHVVTASVDQVSFQRPIRVGEVVTLEASVTRAFNTSVEIIVEVFACDIKGHN
                     ARRCNTAYYTFVALDGENGSPVPVPPVTPLNEIEQQRYDTAPRRRELRLMLSGRMKPE
                     DAHEIKSLFAGVSV"
     assembly_gap    4810..4828
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4829..>5161
                     /locus_tag="PK198_17060"
     CDS             <4829..>5161
                     /locus_tag="PK198_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRF40506.1"
                     /translation="LNGGQVIPSVNNSARGIVTVLISEDRTHISINGVFHGLSGPVTA
                     CHIHTGSPTANGPVLINLSNNVSGNRLRADIPIPTVFWQKAFIPELYIDVHTAANPNG
                     EIRGHLELM"
     assembly_gap    5162..5208
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5209..6539
                     /locus_tag="PK198_17065"
     CDS             <5209..6539
                     /locus_tag="PK198_17065"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRF40507.1"
                     /translation="ASEVSPVSTSASGLGLLAYSPGNFTIRYYIVVNGLSGPITAAHI
                     HSGAIGVNGPVVTPLSQGLPNVLSGTLSTSSLPVDFFQKLIAGTLYVNVHTNAHPGGE
                     IRGQLSLTKGLTFESLLNGDQEVPPVNSAASGAALAFLSPGLDSITYHVIASGLTPTT
                     AHLHQGAAGVSGPVTVALQTTSLPNYYTAKVPATGNFVTQLLNSGIYVNVHTAAHPGG
                     EIRGQLEPLLKRVYAFDICSDQVVPPTGSTAIGAAYFSTDRLNSHFVYRYIVSGLSGP
                     ATFARVHEGAPGTNGLIFFSVNLPNPVGSGQFPITGNEVQKFESGNSYLIVQTAAHPD
                     GEIRGKIMRGLSCSINVSVEDQIVGGSALYPNPTSGETILQLQIKDSFEGELVVNDLH
                     GRLIRKENHAFIPGEFIMQLQLSDTSPGLYVIQIRSAAAGVVWASKLIKH"
     gene            6536..>7290
                     /locus_tag="PK198_17070"
     CDS             6536..>7290
                     /locus_tag="PK198_17070"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRF40508.1"
                     /translation="MKNNTTEFLNGFKMCFLSILKLRFFRTVIAVSMALNLGLASGQA
                     QSSHSYPSELREWRANRIAALRAPQGWLSLAGLLWLKEGDNSFGSDAGNKVVFPAAAP
                     ARVGAFRLQNDSVWMSALPEAGVQFQNKPVTELLLWPANAMPNMGRFSWVVIKRGTQY
                     GVRLWDAEHPNLQGFEHIPHFPVRKKWRIPARFEAYSEPRILLVPNILGMNIEQQSPG
                     VLHFKVSGRDLQIPVLEEGPESFFLVFADATTGA"
BASE COUNT         1621 a         1743 c         2079 g         1573 t
ORIGIN      
        1 attgcggaaa gccggagggg cgcgactgga agccgtcgcg gagttggcgc agggtagaaa
       61 tgagcacgtc gtcgtacagg gcatctactt catcgaggag gagtaccaat ggtttttcca
      121 agcgattgca ccattcggtc aggtatcttt gcagcagaac ggagcttttt gaataattgt
      181 cgggatgtgg gggcatattg ctctcatcca cgaacaggct tgcccgatta tacatggatt
      241 gtatgaaaac atgattggca tcctccacgg aaatgctctg atacccggca gattccaacg
      301 aacacactgt ggctacatac ttcccttccc ggttgagccg atgcgccagc gagtgcaaaa
      361 aggtggtttt gcccgtttga cggggcgcgt ggatgaggaa atactgcttg gcttcgatga
      421 gccgcaaaat gtcatcgtat atatcccgaa agggattgac catgtagtga tcctttgggc
      481 tacacaaccc ggtggtattg aagtatcttt tcatagtggc aaagatagca attatgaaac
      541 gtttttttaa gacataccca gcgctgcgcc cttcatcctt tccgccaatt cctgcgcccg
      601 ctgctccgaa acgctttcgg cgtaaatcct aatgatcggc tcggtgttgg agcggcggag
      661 gtgtacccag ccgtcggcga aatcaatttt gaggccgtcg cttacgtcgc aggattcgtg
      721 gcggaactgc tcgtgcaatt gggagagcag ggcgttcatg tcggtgtcgg gttgcaggct
      781 gattttgtct ttgatcataa aataatcggg ataacggcgc ctcagttgcg agatgctgtt
      841 gcccgaaagg gccagatgcg aaagaaacga cgccgccccg gccagggcat cgcggccata
      901 atgcagttcg ggtacaatga tgccgccgtt gccctcgccg ccgatgacgg cctgcaccgc
      961 tttcattttc tgtacgacat tgacctcgcc caccgctgct gcaaagtact ggccgccatg
     1021 ctgttgcgtc acatcgcgca gtgcgcggga tgaagacaga ttggaaaccg tattgccctt
     1081 gcggcggctc aggatgtagt cggccaccgc caccaaagtg tattcctcgc cgaaaaagct
     1141 gccgtcttcg cacaccaaag ctaagcgatc cacgtcgggg tcaacggcaa tgccgagatc
     1201 ggcgccgagg ttgcgcacgg cctccgaaag tccgccgaga tgttcgggca ggggttcggg
     1261 attgtgggca aaacggccgt tgacctcgcc gttcaggata tatacttcgc agcccagcgc
     1321 ctccaggatg gcgggcatgg ctatggcgcc ggtagagttg acggcatcca ccaccactct
     1381 gaagcggcga ttttgtacgg cctccacttc cacgtcgggc agggccagaa tgcacgccac
     1441 atggtagtcc aacgcggtat catcgctgcg gcaggcgccg aggcgatcca cttcggcgta
     1501 ggtgatgccg ccgttttgga gcagggcgag gaaggcttca ccgtcggcct gggagatgaa
     1561 ttcgccggag gcattgagga gtttgagcgc gttccagtgt gcggggttgt ggctggccgt
     1621 gaggatgatg ccgccgccgg cttgttcacg gggcacggcc atttcgacgg tgggcgtggt
     1681 ggagaggccc aggtcaatga cctcgatgcc caatgccagc agggtttgtg tgaccaatgc
     1741 gccgaccatg gggccggaga tgcgtccgtc gcggcccagt acgattttgg gggcggcgcc
     1801 ggtttggagg agccaggcgc cgtaggcggc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 ccaccgcata ccgaaaaccc cgcgccatgg cgcggctacc gaactcacct tctttttttc
     2101 tggcggatgc gggggtggct ccattcttcg cgttgagcga attcgacgat gcggcgggcg
     2161 atgttcatga tggaaataaa aacgaggtag ccgaacgtga gtaccacaaa aatccagcgg
     2221 taagaaccgg cctcgccgat ggaaagcgaa gaaaaagcat aagccatcaa acctgagccg
     2281 agcgccagtc ccagaaaact gtaaatagag cggccccagt actttacgcc gtcggcagcg
     2341 aagagggaag caacggaatt gaagatggaa aaaaggagca gaaaggccgc cgccgccatc
     2401 caggggaaac ggggagtgac ttgcataccg ctgatttgca ggagtttggc caacaaattg
     2461 acaaccaaca cgccgccaag acagagcgct gccatgacta caggatgaga gggattgatt
     2521 cgactgaaca tgttagagaa ttgtgtgcaa tagaacacga atgtacaaca attgttaata
     2581 gcccgtatct tcgcgcccgc cttgtgcgcc aggggcagac gcggtaccgc agccggatag
     2641 aggaataggc ggtggggctg cggtaagagc agatgacccg acccgccggc ttggtgctgg
     2701 atggtcggag gggtggctaa gcgagtggcg gggggcgccc aaaatatatt ggaagacaaa
     2761 atgccgaaag taacatatcg caatctgggt cgcatatcct accgggaggc gtggagcctt
     2821 caggaggctt tgttgcggga gttggtggac cgaaaattgg ccaaccgaac gcggacggcg
     2881 gaggggctgc cggcagagga gcagcagcac tatttgttgt tttgcgagca tgcgccggtt
     2941 tatacgctgg gcaaaagcgg ctcggaggac aatctgctgt tagatgcaga aacacttgaa
     3001 aaagaggggt ttgagttttt taaaatcaac cggggcggag atattacgta tcacgggccg
     3061 ggccagatag tggggtatcc gatttttgat ctcgactgct ttttcacgga tgtacacaaa
     3121 tatgtgcgct atctggagga ggcggtcatt cgcacactgg cggagtacgg catagcggcg
     3181 gggcgcgagc agggctatac gggggtgtgg ctggagggcg acggcacgtg gctgctctcg
     3241 agcgagctcg gtgaccgcaa gatcggcgcg atcggcgtgc gcatcagccg ctgggtgacg
     3301 atgcacggtt ttgcctttaa tgtgaacact gatctggggc attttaaaaa tatagtgccc
     3361 tgcggtatag ccgatgcgga taaggatgtg acctcgatgc agcgggaact ggggcgggtg
     3421 atggatatgg aggagctgcg ggagaagttg agggggcatt ttgcacaatt gtttgaattt
     3481 gaatggaaaa cgatatgaaa aaagacattc taatacacaa agtggaagac ctggcgattg
     3541 ccattgtgcc gcgcgatgaa agcttggagg acaaagagga actctgggac gtgtttttga
     3601 tcaatctgaa agaggagccg atcacttgtg tgctggtgaa ctcgacggga tacagcgagc
     3661 agcagggcgg ggagcagagg cgaacgacga cgttgcgcca tttttttgag gaggtgggac
     3721 cgctgagcgt ggtacaggtg gagccgattc aggtgcaggt gtttgatctg acgaatgagt
     3781 actgggtgag ttttttgcac gacaactaca tgtatgacaa gaagtatgtg tttgtacggg
     3841 gcagcatccg accggagcat tttatgatgg tgccttttat taacaggaag ggggtggtga
     3901 ttcggtaggc agtgggcgat tccccccccg aaacttttta acgcgaattg gagcgaaggg
     3961 cgcgaaattc cgcgaacgca agggaaacgc ggaacctgga acgcgaaacc cggaactttg
     4021 aaccgggatg aactacaaaa cacaaaattg aaaaaccttg aaggccaaaa aagtatctga
     4081 atcgaacacc gtgatgacgg aaatggtgat gcccaacgac accaacccga tggggaattt
     4141 gatgggcggc aatttgttgc gctggatgga cattgcctgt ggcatttgtg cgggcaagca
     4201 ttgcgaggcg catgtggtga cggcctcggt ggatcaggtg tcgtttcagc ggcccattcg
     4261 cgtgggcgag gtggtaacac tggaggcctc cgtgacgcgg gcgttcaata cctcggtgga
     4321 gatcatcgtg gaggtgtttg cctgcgatat caaagggcac aatgcgcgga ggtgcaacac
     4381 ggcgtactac acgtttgtgg ccctggacgg cgaaaatggc agtcccgtgc cggtgccgcc
     4441 tgtgacgccg ctgaacgaga tagagcaaca gcgctacgat acggcgccgc gcagacggga
     4501 acttcgtctg atgctcagcg gcaggatgaa gccggaggat gcgcatgaga tcaagagtct
     4561 gtttgcaggg gtgagtgtgt gaggggaaaa attgagcttt tcatttgttt gcgatgaaat
     4621 gattacaatg attatcatac atttgtaatc agaaggctgt tggaaaagcc ttttccattt
     4681 caccaaaaac ctgatgacca tgaaaactca tttttcatcc cttttcccgt gggctttcct
     4741 gttgtttttc gtgctgatgt tggcacataa caacgcagcc gcccaaattt tccgcaacaa
     4801 caggatactn nnnnnnnnnn nnnnnnnnct caacggaggg caagtgatac cgtctgtcaa
     4861 caactctgcc cgtggaattg tgaccgttct gatttccgag gatcgaactc atatcagcat
     4921 caacggggtg tttcacggtt tgagcggtcc ggttaccgcc tgccatattc ataccggatc
     4981 gcctactgcc aatggcccgg ttttaatcaa cctcagcaac aatgtgagcg gtaacaggct
     5041 gcgcgccgat attcctattc ccacggtttt ttggcaaaaa gcattcatac ccgaacttta
     5101 catagatgtg catactgccg ccaacccgaa tggtgaaatc cgtggtcatc tggaactgat
     5161 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gcttctgagg
     5221 tatcgcctgt tagcacatcg gcttccgggc tggggctgtt ggcctactcg ccgggcaatt
     5281 ttacgatacg ttattacata gttgtcaacg gactatcggg gccgattaca gcagcgcata
     5341 tccactccgg agccatcggt gtaaatggac cggttgtaac cccgctttct cagggcttgc
     5401 ctaatgtact ttccgggacg ctcagtacat cctcgctgcc tgtggatttt ttccaaaaac
     5461 tgattgcagg aacgctctat gtcaacgtcc atacgaatgc ccatcccggt ggtgaaatac
     5521 gaggacaatt atcgcttacc aaaggtctca catttgagtc tctgctcaac ggcgaccagg
     5581 aagttccccc agtcaattca gcggcttccg gtgcagccct cgccttttta tcccccggcc
     5641 tggactcaat aacatatcat gtaattgcct ccgggctgac ccccactacc gcgcatttgc
     5701 atcagggtgc cgcgggcgta tcaggccctg ttacagtggc gctccaaacc acctcgttgc
     5761 ccaactacta taccgcgaaa gtgcccgcga ccggcaattt cgtcactcaa ctgctcaata
     5821 gtggaatata tgtcaacgtt cacacggcgg ctcatcccgg aggagaaatt cgggggcaac
     5881 tcgagccgct gttgaagagg gtgtatgcgt ttgatatttg cagcgatcag gtagttcctc
     5941 ccacaggttc gacagccatt ggcgcggctt atttcagcac ggatcggctc aacagccatt
     6001 ttgtttatcg ctacattgtc agcggccttt cggggcctgc cacttttgcc cgtgtccacg
     6061 aaggagcgcc cggcacaaac ggccttatct ttttcagtgt caatttgcct aatcccgtag
     6121 gcagcgggca attcccgata accggtaacg aggtgcagaa gtttgagtct ggaaattcat
     6181 atttgatcgt gcagaccgca gcgcatcccg atggtgagat cagaggcaaa atcatgcgcg
     6241 ggctgtcatg ttccatcaat gtttccgttg aggatcagat cgttggagga agtgcgcttt
     6301 accctaatcc aaccagtggg gaaaccattt tgcaactaca aatcaaggac tcatttgagg
     6361 gagaactggt tgtaaacgac ttgcatggcc gcctgatacg caaagaaaat cacgccttca
     6421 ttccgggaga attcatcatg caattacaat tatcggatac atcgccaggg ctttatgtta
     6481 ttcaaatcag atcggcggcc gctggagttg tttgggcatc aaaattgata aagcattgaa
     6541 aaacaatacg acagaatttc tgaatggatt taaaatgtgc tttttatcca tcctaaagtt
     6601 gcgctttttc agaacggtca tcgccgtttc aatggctctt aatcttggct tggcatctgg
     6661 gcaggctcaa tcttcccatt cctacccatc cgaacttcgg gaatggcgcg ccaaccgcat
     6721 cgccgcactc cgggcgccgc agggctggct gtctttggcc gggctgttgt ggctaaaaga
     6781 gggcgacaac agtttcgggt cggatgccgg caataaggtc gtttttcctg ctgccgcgcc
     6841 ggcgagggta ggcgcctttc gactacaaaa cgattcggtg tggatgagcg ccctgccgga
     6901 agcgggcgtg caatttcaaa acaaaccggt gacggaactg ctgctttggc cggccaatgc
     6961 catgccgaat atgggccgtt tttcttgggt ggtcatcaag cggggcacgc aatacggcgt
     7021 ccggctctgg gatgccgagc acccaaatct tcagggcttt gagcacatac cgcatttccc
     7081 ggtcagaaaa aagtggcgca ttccggcccg gttcgaggca tactccgaac cgcgtatctt
     7141 gttggtaccc aatattctgg gcatgaacat agagcagcag agtccgggcg ttttgcactt
     7201 taaagtgagc gggcgcgatc tgcaaatccc cgtattggaa gaaggccccg aaagtttttt
     7261 cctcgttttt gccgatgcta ctaccggcgc
//
LOCUS       DAORLF010000238         7272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5548_length_7272_cov_1.928502, whole genome shotgun sequence.
ACCESSION   DAORLF010000238 DAORLF010000000
VERSION     DAORLF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_7272_cov_1.928502"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="rfaD"
                     /locus_tag="PK198_17075"
     CDS             complement(<1..861)
                     /gene="rfaD"
                     /locus_tag="PK198_17075"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRF40509.1"
                     /translation="MIIVTGAAGFIGSCMARKLNDEGHRNLILSDDFSREDKNRNFTG
                     KSYIAAVHRDQLPDWLERNHADVEAVFHLGARTDTTEQDVALFDRLNLHYSQAIWNLC
                     ARHGIPLIYASSAATYGLGEHGYRDDHDIVGQLQPLNPYGRSKNDFDIWAMQQQDAPP
                     FWAGLKFFNVYGPNEYHKSRMASVVFHTARQIKATGGMKLFRSHRPDFADGMQSRDFI
                     YIKDVLDICYWLFRHHPASGLYNVGTGQARPFLHLAENTFRAMGLEPNISFIDTPEDI
                     RETYQYFTEAD"
     gene            complement(871..1539)
                     /locus_tag="PK198_17080"
     CDS             complement(871..1539)
                     /locus_tag="PK198_17080"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF40510.1"
                     /translation="MKNYITFLLFALLSGSLPAQSGIELGMMGGLSFYQGDLTNSLVW
                     ELKESNIGAGLFARFPVNDSWNMRAGLSYVRIAGTDLDNADRDRRAFAFHNNMVEFSL
                     VGEFEPWGSNRIAGGLPVSPYLVFGSGLLYTKPKVDFSQFTGDLLTRNVIRDRDTPYS
                     KLRVIIPVGVGVKVPMGDSWLVGLEAAIRYPFTDYLDGISYSANPEKNDWYALIGLTL
                     SKKL"
     gene            complement(1594..3231)
                     /locus_tag="PK198_17085"
     CDS             complement(1594..3231)
                     /locus_tag="PK198_17085"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF40511.1"
                     /translation="MKKIILGAILLLPLILTGQAKWEFGLTGGGWMYMGDLAPSRFPT
                     LKELQPGGGALLRRRLGETMALRGSFLSGSLSGTDRNIRSKDIRKVRLIGFESKIMDA
                     SVVLEWEPWAGRRYPGNFQFKKLISPYIFAGGAWYRNEVQNDYSAMPSDGLWGAVRKD
                     QTTEYKNTGWSIPFGGGVHFDLSRRSVVSVELTMRRAANDYLDGVSYAGNPDNKDWYA
                     FAGVSYNYRFTPRDRDKDLIPDKQDFCPAVNGALSAMGCPDADGDGLEDVEDMCPNAP
                     GPRVANGCPDTDGDELPDHLDDCPLYAGPEKTKGCPDADDDGIPDKDDECPQAGIADY
                     KGCPDTDGDGFPDNKDDCPEEAGVPEKQGCPYRDTDGDGIFDDFDECPDAAGPKELGG
                     CPDTDNDGIADKNDRCPEAAGTKELAGCPEMSEEHKKILAAARWGVEFETASATLKPH
                     SANVLNKIAGLLNDYPVYHLHISGHTDNRGVPAKNQTLSEKRAKSCVDYLVGKGVTAE
                     RLRSEGFGAGKPIATNNTAQGRSRNRRVDFDLVVPAQ"
     gene            complement(3493..3885)
                     /locus_tag="PK198_17090"
     CDS             complement(3493..3885)
                     /locus_tag="PK198_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40512.1"
                     /translation="MTNTITLSSNMDSLILIDEGKAAIHNLSHPNGLIIGTLRGQIVA
                     GTSESLPEATEVVKTITLSLEAERWTIFYDRPGKILNVIGATDIDLGLVGDREAPSNA
                     VIHRIAIGGNPVHVRRDGNTITLSLLKV"
     gene            complement(4011..4481)
                     /locus_tag="PK198_17095"
     CDS             complement(4011..4481)
                     /locus_tag="PK198_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRF40513.1"
                     /translation="MDQLDSLNQVATFHRTFHHPVEPRPVIPAPERCRLRVSLLAEEL
                     SELEEAIAENDIVAVADALCDLQYVLSGAVLEFGLGEKFRALFDEVQRSNMSKACRNP
                     AEAEATVKYYEEERGVPCYYEEDNGLYLVYRKEDRKTLKSVFYSPADLQQIVES"
     gene            5328..6092
                     /locus_tag="PK198_17100"
     CDS             5328..6092
                     /locus_tag="PK198_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF40514.1"
                     /translation="MELNYKSFGQGEPVIILHGLFGTLDNWQTIAKALADEYLVFIVD
                     QRNHGRSPHVPGMSYSLLAEDLREFMESHWIYRAHIVGHSMGGKTAMQFALIYPDMVD
                     RLAVIDIAPKAYAGGHEAIFDALLSLPLDEVESRQEAEAFLAARIDDPGVLQFLLKNL
                     SRSTEGHYAWKMNLPVLHQYYHKILDTVAGDAPYEESVLFLRGGRSQYIQLPQDEPAI
                     RALFPEARIETVPGAGHWVHAEAPQAVVEHLKLFLG"
     assembly_gap    6389..6398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6399..>7272
                     /locus_tag="PK198_17105"
     CDS             <6399..>7272
                     /locus_tag="PK198_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF40515.1"
                     /translation="DFDGSFSLSSPAGNVEVTVSFVGYQTVKQNITLASGETRTLDFA
                     LEIETSVLKTATVTSGKFARPLSEETVSIEVLKPSLINNTGKVSLDKALEKIPGVTII
                     DGQANIRGGSGFSQGAGSRVLLLVDDIPILQADAGFPNWGDVPVENIEQVEVLKGAAS
                     ALYGSSALNGIINVRTAFARSQPETGGALWATSYFSPKEKSLKWWGVDSLPQPFTYGA
                     NVYHRQKFNKFDLVLGGFYQNEQSFNKDTYNRYGRFNFSTRYRFTDRLSAGINGNFNG
                     GESGSFFYWKSAANR"
BASE COUNT         1711 a         1821 c         1891 g         1839 t
ORIGIN      
        1 atcggcttcg gtaaaatact gataggtttc gcgaatatct tcaggggtgt ctataaaact
       61 gatgttgggt tccagtccca tagctctgaa cgtgttctcg gccagatgca ggaagggcct
      121 cgcctgtccg gtaccgacat tgtacaaacc cgaagccggg tggtgtcgga agagccagta
      181 gcaaatatct aacacatctt tgatgtaaat aaaatcgcgg ctttgcatgc cgtcggcgaa
      241 atcggggcgg tgcgagcgaa acagcttcat gccgccggta gctttgatct ggcgcgccgt
      301 atgaaacacc accgacgcca tgcggctttt gtggtactca ttggggccat acacattgaa
      361 gaatttcagt ccggcccaaa acggcggcgc gtcctgctgc tgcatcgccc agatgtcgaa
      421 gtcgtttttg gaacgcccat acggattgag cggctgcaat tgccccacga tgtcgtggtc
      481 gtcgcggtag ccgtgctcgc ccagcccata agtggcggcg ctggaagcat agatgagcgg
      541 gatgccgtgt cgggcgcaca gattccatat tgcctgggag tagtggagat tgagccggtc
      601 gaaaagtgcc acatcctgtt cggtggtatc ggtgcgggcg ccgaggtgaa aaacggcttc
      661 cacatcggca tggttgcgct ccaaccagtc gggcagttga tcgcggtgta cggcggcgat
      721 gtagcttttt ccggtgaagt tgcggttttt atcctcccgg ctgaaatcgt cggaaaggat
      781 gaggttgcga tgcccctcgt cgtttagttt gcgcgccatg cagctcccga tgaagcccgc
      841 cgcgccggta acgatgatca taatttgttg ctaaagtttc ttggacagcg tcagcccaat
      901 gagcgcgtac cagtcattct tttcaggatt ggccgagtag ctgatgccgt ctaaataatc
      961 agtgaaaggg tagcgaatgg cggcctccaa accaacgagc caagagtcgc ccatcggcac
     1021 ttttacgcca acgccgacag gaatgatgac cctgagcttg gagtagggcg tgtcgcgatc
     1081 gcggatgacg ttgcgcgtga gcaggtcgcc ggtgaattgg gagaagtcca ctttcggctt
     1141 ggtgtaaagt agcccgctgc cgaagactaa gtagggcgag accggcaggc cgcctgctat
     1201 gcggttgctg ccccagggtt cgaactcgcc tacgagcgaa aattccacca tattattatg
     1261 aaaagcaaaa gcccggcggt cgcgatctgc attatcgagg tcggtaccgg caatgcgcac
     1321 ataagacagc cctgcacgca tgttccagga gtcgttcaca ggaaaacgag caaacagacc
     1381 tgctccaatg ttggattctt taagctccca tacgaggctg ttggtcagat cgccctgata
     1441 gaacgaaaga ccgcccatca tccccagttc aatgcccgat tgtgcgggca aggagccgct
     1501 caagagtgca aaaagcaaaa atgtgatgta atttttcata gtatgtgcag attgaagccc
     1561 caaaagtact ggctttttcg caggcaggca aaattattga gcaggaacta ccagatcaaa
     1621 atccacgcga cgattgcggc tgcgtccctg tgcggtattg ttggtagcaa tgggcttccc
     1681 ggcgccgaat ccttctgacc ggagccgctc ggcagtcacg cctttgccga cgaggtaatc
     1741 cacgcaactt tttgcacgtt tttccgaaag cgtctggttt ttggcaggta cgccccggtt
     1801 gtcggtatgc cctgaaatgt ggagatgata tacaggataa tcgttgagca gccctgcaat
     1861 tttgttcaaa acattggctg aatgcggctt gagcgtggcg ctggccgtct caaattcaac
     1921 accccagcgg gcagccgcca atattttttt gtgttcttcc gacatttctg ggcaaccggc
     1981 caattctttc gtgccggcgg cttccgggca gcggtcgttt ttatcggcta taccatcgtt
     2041 gtcggtatcc gggcagccgc ctaattcttt ggggccggcc gcgtcggggc actcatcgaa
     2101 gtcatcaaat atgccgtcac cgtcggtatc gcggtatggg cagccttgtt tttcaggcac
     2161 gccggcttcc tccgggcagt cgtctttgtt gtcggggaaa ccgtcgccgt cggtatccgg
     2221 gcagccttta taatcagcga taccagcctg cgggcactcg tcgtctttgt cgggtatgcc
     2281 gtcatcgtca gcgtcggggc agcctttggt tttttcggga ccggcgtaca gcgggcagtc
     2341 atccaaatga tcgggcaatt cgtctccgtc ggtatccggg caaccgttgg ccactctggg
     2401 gcctggcgca ttgggacaca tatcttctac gtcctccaat ccatcgccgt cggcatccgg
     2461 gcagcccata gcgctcagcg cgccgttcac tgcggggcag aagtcttgtt tgtcgggtat
     2521 caaatccttg tcgcggtcgc ggggcgtaaa acgataatta taggagaccc ctgcaaacgc
     2581 ataccagtct ttgttgtcgg gattgcccgc atagctcacg ccgtccaaat agtcgttggc
     2641 tgcgcggcgc atggtcaatt ctacgctgac gacgctgcgc cggctgaggt cgaaatgcac
     2701 accgcctccg aaaggaatgc tccatcctgt gtttttatat tctgtggtct gatcttttcg
     2761 taccgcgccc cacaggccgt cggaaggcat ggcggagtaa tcattttgca cctcgttgcg
     2821 ataccaggcg ccaccggcga agatataagg cgagatcagt tttttgaatt gaaaattgcc
     2881 cggatagcgg cggccggccc agggttccca ctctaatacc accgaggcgt ccatgatttt
     2941 gctctcaaac ccgatgaggc gtaccttgcg aatatctttg cttcggatat tgcgatctgt
     3001 accgctcagg cttcccgaca ggaaactgcc ccgcaaggcc attgtttcgc ccaaacgacg
     3061 gcgaagcaga gcgccgcccc ccggttgtaa ttctttcaga gtagggaaac gtgagggagc
     3121 caggtcgccc atgtacatcc atccgccgcc ggtcagccca aattcccatt tagcttgtcc
     3181 tgtcaggatc agggggagca gaagaatggc tcccaaaatg atttttttca tgtagttggt
     3241 atgcttaaac gcctcccgat tgcagcttga atgcctccct gctcatcggt aaataagtaa
     3301 ttgtgttgtt ccgagacgtg ggggaacgct ttgaacagct gcaaaggtac aaaacataga
     3361 aaaaaggaga ataagtcttt tgttaatgtc ttttgtgacg ctgcattatt tctgctgcat
     3421 ggttatatcg ggcaaagaaa tcctctccgg acagtgtgtg ctttccggtt caatatttta
     3481 acgaattaaa aatcagacct ttaacaaaga gagcgtgatg gtattgccgt cgcggcgaac
     3541 atgtaccggg ttgccgccga tggctatgcg atggatgacg gcattgctcg gcgcctctct
     3601 atctcccacc aaacccaaat ctatatcggt agcaccgatg acgttgagga ttttgcccgg
     3661 gcggtcgtag aaaatagtcc aacgctctgc ctctaaagag agggtaatgg tttttactac
     3721 ttctgttgct tccgggaggc tctcggaagt gccggccaca atctgtccgc gtaatgtccc
     3781 aataatcagg ccattagggt gcgagaggtt gtgtatggca gctttacctt catcaataag
     3841 aatgaggctg tccatgttgg acgaaagggt aatggtgttg gtcataatcg ttcgtttatt
     3901 ggcagatgta acttctggtg aaaggatagg tagtttcttt ttcaacgcca tagctcaaag
     3961 tgtttgtggc acaaagctaa aaaatcaatc cgtgtggtta tcaataaatt ttacgactcc
     4021 acgatctgct gcaaatcggc cggcgagtag aagactgatt tcagcgtctt ccggtcttct
     4081 tttcgataca ccaaatacaa accgttgtct tcttcgtaat aacagggcac gccgcgttcc
     4141 tcttcgtaat atttaacggt ggcttctgcc tctgccggat tccggcaggc tttgctcatg
     4201 ttggagcgct gtacctcatc aaacaaggcg cgaaattttt ctccaagccc aaactccagc
     4261 acggccccgc tcaatacata ttgcaggtcg cacagagcgt cggctacggc cacgatgtcg
     4321 ttttcggcaa tcgcctcctc caattcgctc agttcttcgg ccagcaacga cacacgcaac
     4381 cggcagcgtt cgggcgccgg aatcaccgga cggggttcta caggatgatg aaaagtacgg
     4441 tgaaacgttg ccacctggtt gagcgagtcg agttgatcca tgttctgttg agttgatttt
     4501 tcgcctcgca aggtagggcg aagtacccga ttttgaaaaa aaatgggctt tgaacagaaa
     4561 aaaaataaaa aaaacaggct aaaaaaaaca ggtgaaagat tcttttattt tcacaaattc
     4621 tggatattat gatctttact aattctttaa tttcttgtat ttttaataaa aatgtatgaa
     4681 tatttttttc acaattatca ttttttgtct ttgtgcaggg ttagagaccg ctgcatattt
     4741 gtatcgtgat tgagaacaag aggagacaga aacaaaaacg gcgggttcaa aagtggcctg
     4801 cgacaattga aagaaatatg ttcatgggat tataatttgt tggtggtaag tcgtcccgag
     4861 ccgctcgttc gggacgattc acacttttta tagtctctgc gtaaagatag atttcgcacc
     4921 aaaataaaaa agcgaaccga tttcggttcg cctcaaaaat aggtcatgct catgggatta
     4981 cgaaaaacta tcagcaaaaa tttgagatca caatttgtta aagtggatta gtactccctc
     5041 ttttttaaaa cgatggtgcg aaattatacc gattatttgt tctgcgcaag ttttcagtgc
     5101 aaataatttc ggaaaatttt ttccacagat tttggatatg ttcatgggat tataagattt
     5161 gaaaagtcgt gccatcggta cgactttttt tttgccccta tataaccccg attttcatgg
     5221 tttctgtcgg ctgctttcgc cggacatatc ctattgacgc cgagttggaa aggaagtcac
     5281 acaccatcat atatgttttt ttctcacgcc tttgataact ttaccgcgtg gaactcaatt
     5341 acaaatcatt tggtcagggt gaaccggtca tcatcctgca cggcttgttc ggtaccttag
     5401 acaactggca gaccatagcg aaggctttgg ccgacgagta tctggttttt atcgtagatc
     5461 agcgcaatca cgggcgctct ccgcacgtac ccggcatgtc ttattctttg ctggccgaag
     5521 acctgcggga gttcatggaa tcgcactgga tataccgggc acatatagta gggcactcaa
     5581 tgggcggcaa aaccgctatg caatttgcgc ttatctatcc ggatatggtg gaccgtttgg
     5641 ccgtcataga catagcgccg aaagcttatg caggcgggca cgaggctatt ttcgatgcgt
     5701 tgttgtccct gccattggat gaagtggaaa gccggcagga agcagaggcg tttctggcag
     5761 cgaggattga cgacccggga gtcttgcagt ttttactcaa aaacctcagc cgaagcaccg
     5821 aaggccatta tgcctggaaa atgaatctgc cggtgttgca ccaatactac cacaaaattc
     5881 ttgataccgt agccggtgac gcgccctacg aagagtctgt tttgtttctg cgaggcggcc
     5941 ggtcgcaata catacaactt cctcaggatg agccggctat tcgtgcgctc tttcctgaag
     6001 cccgcataga aaccgtgccc ggcgcagggc attgggtaca tgcagaggcg ccccaagccg
     6061 tggtggagca tctgaaatta tttctcggct aataaaacac gtctgctccg aagagtgaaa
     6121 atctcctcgg atatagtttt ttcaaaatct tactttagat ttgctccccg ttttgctcct
     6181 ttgaacatag tcaacttttt tacaaacgac tattaaacag aattttatga gcaaattttt
     6241 ctcttgggtg ctgctcctcg caagcctctt tgcatccatt tcagcaactg cacaaaacgc
     6301 cgttttacaa ggcaaaatca cagaccgcga aaatggcgaa cccctgcccg gcgccaacgt
     6361 aagcatcggc ggcggcggta ccgtttccnn nnnnnnnnga tttcgacggc agtttcagcc
     6421 tgtcttcgcc tgccggcaat gtcgaagtta cggtcagttt tgtaggctac cagactgtaa
     6481 aacagaatat tacgcttgcg agcggggaga cccgtacctt ggattttgcc ttggaaatcg
     6541 aaacttcggt attaaaaaca gccacggtga cgagcggcaa atttgcccgg ccgctgagcg
     6601 aagaaaccgt ttcgatagaa gtactcaaac ccagtctcat caacaatacc ggcaaggttt
     6661 ccttagataa ggctctggaa aaaatacccg gcgtgaccat cattgacggg caagccaata
     6721 tccgaggagg ttcgggcttt tcgcagggcg cgggcagccg ggtgttgttg ctggtggacg
     6781 atattccaat cctacaggca gatgccggtt ttcccaactg gggcgatgtg ccggtggaaa
     6841 acatcgagca agtggaagtg ctcaaaggcg cggcttctgc cctttacggc tcctcggcgc
     6901 tcaacggcat cattaatgta cgcacggcct ttgcacgctc gcagcccgaa accggcggcg
     6961 cgctatgggc tacgagctac ttctcgccta aagaaaaatc gctgaaatgg tggggcgtgg
     7021 acagtctgcc gcagcccttc acttatggcg ccaatgtgta tcatcgccag aaattcaaca
     7081 agttcgatct ggttttaggc ggtttttacc aaaatgaaca gagctttaac aaagacactt
     7141 acaaccgcta tggtcgtttc aatttttcca cccggtacag attcaccgac cgcctgagcg
     7201 ccggcatcaa cggcaacttc aacggcggcg agagcggtag ctttttctat tggaaatctg
     7261 ccgccaaccg ct
//
LOCUS       DAORLF010000239         7233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5586_length_7233_cov_1.623015, whole genome shotgun sequence.
ACCESSION   DAORLF010000239 DAORLF010000000
VERSION     DAORLF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5586_length_7233_cov_1.623015"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>929)
                     /locus_tag="PK198_17110"
     CDS             complement(<1..>929)
                     /locus_tag="PK198_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40516.1"
                     /translation="WSASLTDANGCTALAALTLDGAASVTVTQTQQSCRGEPYLFEGQ
                     SYSSDTLLCRTYTGLNGCDSTHCISLVFLDTFYTTETVLLCPDESYDFNGLLLRGDTT
                     ACLILTASNGCDSTHCVSTEVVRRESFNLAAICPGAAYTFNNRVFDQAGTYTEIIPQS
                     TGCDSVAVLQLSVLPPPLVGLSASGSLCRGASVALNVAAGMASYLWSDGSAGANLLTS
                     QPGLYRVTVTDLNGCTASDSILIADEAVRAQWNAEPPICEGLQGSVIASGITGGTPPY
                     RHGPAGGLLQPVTEPVRLPSGPHRMVLEDANGC"
     assembly_gap    930..1004
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1005..>1219)
                     /locus_tag="PK198_17115"
     CDS             complement(<1005..>1219)
                     /locus_tag="PK198_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40517.1"
                     /translation="EPYSFVWFNGAQTDSLSGLGAGMYQLTVTDATGCRATDTVFIPQ
                     PDSIVVSLLPSAMFVCDSTGSISANASG"
     assembly_gap    1220..1392
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1393..>1626)
                     /locus_tag="PK198_17120"
     CDS             complement(<1393..>1626)
                     /locus_tag="PK198_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40518.1"
                     /translation="LVRVGLPIDSMRISLSGILNAGQEYLELSAAPHLSVFGSGTTTL
                     TLVDDGTATFADFEAALSAIRYYNTAPAPAYGT"
     assembly_gap    1627..1640
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1641..2220)
                     /locus_tag="PK198_17125"
     CDS             complement(<1641..2220)
                     /locus_tag="PK198_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40519.1"
                     /translation="MAINGTQVFATSPNDTILNAMTCDYEGLVYAAGQGISVFNPADS
                     SISYRGPLPPGLASAGGMTYRNGEFFLSTVSNSLARVDVENPANTVEIAQFPPGTPPI
                     DALVSFPYRCDSIVTYAFARAESSTTIYRLDFDDYSLSEVCVFDRAITAAAAREECIQ
                     PPCHFTIDLDAQDLSGLPGQDFRADTACFGPLP"
     gene            complement(2568..>3445)
                     /locus_tag="PK198_17130"
     CDS             complement(2568..>3445)
                     /locus_tag="PK198_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40520.1"
                     /translation="VFGFHGELWLYARICGSEYGEISGTPIIPSGGITIGGQQYNCPS
                     STNCDICRAIEIPNQRCFFNVECVIFERDEDGNIIETHTTIEHEAVPGNIKFVKRKVC
                     YRGELPSESQGGCDCAIYQVCDMPCNPWSRPVRVHTLLNIDCEDEVFIPQLNLNLENI
                     DFWGEPCMDVTPGIASEEELEFRQPENQVHRTPHVSILTDTSEGTFVTAYPNPFDRKL
                     NIQFSTTTHSSWQVRLSQLSGQLILEKELRPDAGEFIYELEIGHIILPGVYLLSITDN
                     HGRRFMQKVVKAAGQ"
     assembly_gap    3446..3505
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3506..>4150)
                     /locus_tag="PK198_17135"
     CDS             complement(<3506..>4150)
                     /locus_tag="PK198_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40521.1"
                     /translation="EEEDVSLVFFRRFSADRVFIEWGNNQTSLIQLNNNGNISNIIYG
                     TDRMTIDPPGDLSAIVTDEKGCKNTVSYFAGGAENTFLRLEAYEIDDHLADFGFTYEN
                     FVNAISPPIPEINPVPISGFDVCGYCTRSFNGSISFIGAGECNFAEFAFSSNPEGVNP
                     FAENICQRGGRINCFAPSDADRFSWRVPPNFEGIYVNMEPNLGPVFDCGCLFPV"
     assembly_gap    4151..4166
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4167..5064)
                     /locus_tag="PK198_17140"
     CDS             complement(<4167..5064)
                     /locus_tag="PK198_17140"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40522.1"
                     /translation="MNVQGGQEPYSFLWSNGVVTPMAGDLASGSYFVTITENGGCATT
                     IAVQLPQNPSAQLINSFVVQNSCGGPTGSIGVSTVADMEPVTILWDNGTVGPFISGLV
                     PGEYCLIAMSALGCIETACFVVEQISNDLSIIIEEVRGGYPDYIEGTGFIDISVEGGT
                     APYTFSWSTGFTGEDLYQIFPGVYTVTVTSANGCSTSIAIDVPECSSTTLTLSCFPYN
                     VTPIGLQGGSIRVNVMGGTGPYSYEWSGPNGPIPTSHYILDQITVPGNYCLVVRDQCG
                     QLGQICRTMIREDRCSQHVEMFL"
     gene            5069..>5316
                     /locus_tag="PK198_17145"
     CDS             5069..>5316
                     /locus_tag="PK198_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40523.1"
                     /translation="MEPLALWQVAGVITGSTKTSTFSTSANCTTRQPKAFVTVTEYEP
                     GATLNISSVVAPLLHKNEGGNAGGLCTLMSTAPPIPAQA"
     assembly_gap    5317..5394
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5395..>7233)
                     /locus_tag="PK198_17150"
     CDS             complement(<5395..>7233)
                     /locus_tag="PK198_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40524.1"
                     /translation="FGECFQQSQGGVDLSVSGGQAPYAFQWNNGWPLEDLGPVNAGVY
                     KVTVTDATGCTALASFTVAQHTPPDISGQVQLSSCANNADGSIQINIANGTAPFEYEW
                     NNGAVSEDLSNLTGGIYILTVTDDNGCFDQAGFNTMPMTPQDALPYLRRLRITAIHTT
                     TAEETLIYDAEWVEAAGDCLFFLPNETDMPQTVFAQIKNGQRSLRFDAEFSEPMMPAP
                     SMFSMNVLNLTGLPAGGNAWVFTLTTNQAASIVQNGRIAETFVFSGMDLRGNALFDLR
                     TASNQMSDCAALPRFMPDCVWSPAVSGNNEDNVHTLIRDCMALSLSVNHNNNSASATA
                     LGGLAPISWQWEGPDGFTSTASQISGVPPGRYCLTAKDANGCTTEECIDFCKSLEELV
                     SDLLSITPPCPGQSDGMLCLNTNGDFALQVSWPDGSDELCMTGIAAGQSYCFEVTELR
                     CMQTVEHCTDPVMPVSALSLALSSSQPACPGQMNGALTVTAEGGRAPYVFSWASGQSG
                     SMAAGLAAGICHAVTVTDACGQTFHTCFNVPEATPLNINDIVVNNACGTTPTGSIRLQ
                     LSGLGPMTIVWKNKKGDVIGSNQPFINQLLPGPYSVNVTDACGQAVT"
BASE COUNT         1675 a         1786 c         1805 g         1551 t
ORIGIN      
        1 cgcagccgtt cgcgtcttcc agcaccatcc ggtggggtcc tgagggaagc ctaacaggtt
       61 ctgtcacagg ttgcaacaag ccgcctgccg ggccatgccg gtacggcggc gtaccgcctg
      121 tgatgcccga agcgatcacc gagccttgca aaccttcaca aatcggcggc tcggcattcc
      181 attgcgcccg caccgcttcg tcggcaatga ggatgctgtc ggaagctgtg cagccgttga
      241 ggtcggtcac cgtcacgcgg taaagacccg gctgcgatgt gagcaaattg gcacccgcgc
      301 tgccgtccga ccagaggtag gacgccatgc cggcagcgac attcagcgca accgatgcgc
      361 cccggcagag gctacccgat gcgctcagcc cgaccaacgg cggaggcagt acgctcaatt
      421 gcagcaccgc cacgctgtcg cagccggtac tttgcggaat gatctctgtg taagttccgg
      481 cctgatcgaa gacccggttg ttgaaggtgt aggcagcgcc gggacagatg gccgcgaggt
      541 tgaacgattc gcggcgaacg acttccgtgc tcacgcaatg cgtagaatcg cagccattgg
      601 aagccgtcaa aatcaggcag gcggtggtgt cgcccctcag cagcaggccg ttgaaatcgt
      661 agctttcatc tggacagagc agcacggttt ccgttgtgta aaacgtatcg aggaagacca
      721 aagaaatgca atgcgtcgaa tcgcagccgt tgaggcctgt gtaagtgcgg cacagcaggg
      781 tgtcggagct ataagattgg ccttcaaaga ggtatggctc tccccggcag ctttgctgcg
      841 tctgcgtgac ggtgacggag gccgcgccgt cgagggtaag agcggcgagg gccgtacatc
      901 cgttcgcgtc ggtgagggaa gccgaccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgctt gcgttggccg
     1021 atatgctgcc cgtgctgtcg catacgaaca tggcggaagg cagcagcgac acgacaatgg
     1081 aatcgggctg agggataaat accgtatccg tggcgcggca gccggtggcg tcggtgacgg
     1141 tgagttgata catgccggcg ccgaggccgc tcagactgtc cgtttgtgct ccgttgaacc
     1201 aaacaaagct gtacggttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nngcgtgccg taagcgggcg cgggtgccgt gttgtagtag cgaatggcag
     1441 agagcgctgc ctcgaagtcg gcaaaggtgg ccgtgccgtc gtccaccagc gtgagcgtgg
     1501 tagtgccgga gccgaaaacg gagaggtggg gcgcggcact cagttccaga tactcctgtc
     1561 cggcgttcag tatgccggag agcgagatgc gcatggagtc tattggaagg cccacccgca
     1621 ccaaggnnnn nnnnnnnnnn tgggcagcgg cccgaagcag gccgtatcgg cgcggaaatc
     1681 ctggcccggg aggccggaga ggtcctgcgc gtcgagatca atggtgaaat ggcagggcgg
     1741 ctgaatgcac tcctcgcggg cggcggcagc ggtgatggcc cggtcgaaga cacagacctc
     1801 cgagaggctg tagtcatcga aatccaggcg gtagatggta gtgctggact cggcgcgcgc
     1861 aaaggcatag gtgacgatgc tgtcacagcg gtacgggaag gataccaagg cgtcaatggg
     1921 cggggtgccg ggcggaaact gcgcgatctc cacggtgttg gcgggattct ccacatccac
     1981 ccgcgccaga gaattggaga cggtactgag aaagaactcc ccgttgcggt aggtcatgcc
     2041 gcccgccgaa gctaagcccg gcggcagcgg cccccggtac gatatgctgc tgtcggcagg
     2101 attgaatacc gagatgccct gcccggcagc atataccaag ccctcgtagt cgcaggtcat
     2161 ggcgttgagg atggtgtcgt tgggggaggt ggcgaagacc tgtgttccgt tgatagccaa
     2221 aactgaatct gcataaatag catgaaaata tgtaataaat cccccagtga acccatgaac
     2281 ttcaatatcg tttacttgag acccatgaaa tccatacgtc cacacgatat tattgcttaa
     2341 cgtgaactca taataattac tacttgacct aagcctagct cctgtggcgc agttcgccaa
     2401 ctgcaaatag gtgacgtagc tatacattcg agagtcgaaa gcccaaatgt cgtcaaaagc
     2461 aatccatact ttttgggcat gagcggagga ggcggtatag agcaatagta atgtacagca
     2521 gaccctaata cacttcgccg ttgataaaca tattcgaaga tgattcgtca ttgccctgct
     2581 gcttttacca ctttttgcat gaaacgcctt ccatgattgt cggtaatgga caacaaatat
     2641 actccgggca aaatgatatg accaatctct aattcataaa taaattcccc ggcatcaggt
     2701 ctcagctcct tttccagtat caattgtcca cttagctggg aaagtcgaac ttgccatgaa
     2761 gaatgtgtgg tcgtagaaaa ttgaatattg agttttctat cgaaaggatt tgggtaggca
     2821 gtaacgaaag ttccttcgga ggtgtctgtc aaaatgctga catgaggcgt cctatggact
     2881 tgattttccg gctgcctgaa ctccagttct tcttcagatg cgatacccgg tgttacgtcc
     2941 atacacggtt caccccaaaa gtctatattt tctaagttca agttcaattg gggaataaaa
     3001 acttcgtctt cacaatcaat attcaaaagg gtatgcaccc ttacaggccg gctccaggga
     3061 ttacaaggca tatcgcagac ttgataaata gcacaatcac aaccaccttg agattctgaa
     3121 ggtaactcac ctctgtaaca aacttttctt tttacaaact tgatattgcc tggcactgcc
     3181 tcatgttcaa tagtcgtatg cgtctctatg atgtttccat cttcgtcgcg ttcaaagata
     3241 acacattcca cattgaagaa gcacctttga ttaggtattt cgatagcccg gcaaatatca
     3301 caattagtcg aagaggggca attgtactgc tgaccgccta ttgtaatgcc tccgcttgga
     3361 ataataggcg tgcctgaaat ttccccgtat tccgatccac atatcctggc atacagccag
     3421 agttctccgt ggaatccgaa tacatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnntcact ggaaagagac agccacagtc aaatacagga
     3541 cctaaatttg gttccatatt aacatatata ccctcaaaat taggcggtac ccgccaggaa
     3601 aaacgatctg catcagaagg ggcaaagcaa ttaatgcgcc ctcctctttg gcaaatattt
     3661 tcagcaaagg gattaacccc ttccgggttg gaactgaacg caaattcagc aaaattacat
     3721 tcgcctgcac cgataaagga aatactccca ttaaaactcc tggtacaata tccgcataca
     3781 tcaaaaccgg aaataggcac gggatttatt tcaggaatgg gaggagaaat ggcgttgaca
     3841 aagttttcgt aagtgaatcc aaaatctgcc aaatggtcat caatttcata agcttccaat
     3901 cttagaaaag tattttcagc gccccctgca aaatatgata ctgtgttttt gcaccctttt
     3961 tcgtctgtaa caatagcaga aagatcacca ggaggatcaa ttgtcattct atctgttccg
     4021 tatatgatat tgctgatgtt cccattattg ttcagttgaa tcaaactggt ttggttgttg
     4081 ccccattcaa taaatactcg atcagcgcta aaacgacgaa aaaaaaccaa agagacatct
     4141 tcttcttcac nnnnnnnnnn nnnnnnccaa aaacatttct acatgttgcg agcatctgtc
     4201 ttctcgaatc atcgtccggc aaatttgccc caattggcca cattgatctc taacaactaa
     4261 gcagtagttt cccggaacag taatttggtc taaaatgtaa tgagacgttg gaatcggccc
     4321 atttggtcca gaccactcat aactatatgg tccggttcct cccatcacat tcactcttat
     4381 agaccctccc tgtaggccaa tgggagttac attgtatgga aaacagctta gtgtaagtgt
     4441 tgtactgcta cattcaggaa catcaatggc aatggaggta ctacatccat tggcagaagt
     4501 aacggttact gtgtaaacac caggaaaaat ttgatataag tcttctcctg taaaacccgt
     4561 actccatgag aatgtgtagg gagccgtgcc gccctccaca gagatgtcaa taaaccccgt
     4621 gccttcaatg tagtcggggt atcctcctcg cacctcttct attataatag acaggtcatt
     4681 tgatatttgt tctaccacga agcaggctgt ttctatacaa cctaatgccg acattgcgat
     4741 cagacaatac tcgccgggta caagacctga aataaaaggc cctacagtac cattgtccca
     4801 caaaatggta actggttcca tatcggcaac cgttgatacc ccaatactgc cggtggggcc
     4861 gccgcaacta ttttgtacaa caaaactgtt gatcaattgc gctgacgggt tttgaggtag
     4921 ttgaacagct atagttgtag cacagccacc attttccgta attgttacaa aataacttcc
     4981 agaggctaga tcgcctgcca taggagtcac aactccgttg ctccacaaaa aagaataagg
     5041 ctcttgaccg ccttgcacat tcaaaaagat ggaaccattg gctctttggc aggtagcagg
     5101 cgttataact ggttcaacca aaacttccac attctccacc tccgcaaact gcaccacccg
     5161 gcagccgaag gcatttgtca ccgtaacgga atacgagccg ggcgctacat tgaatatatc
     5221 ctcggtagtg gccccgttgc tccacaaaaa cgagggcgga aatgccggcg ggctttgcac
     5281 gctgatgtcc actgcgccgc ccattcctgc acaggcnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttacc
     5401 gcctgaccgc aggcgtccgt cacatttacg ctataaggcc ccggcaagag ttggttgatg
     5461 aagggctggt tgctgccgat gacgtcgcct tttttgtttt tccaaacaat cgtcataggc
     5521 ccaagtccgc tcagttgcag gcggatggaa cctgtgggcg tagtgccgca ggcattgttc
     5581 acaacaatgt cgttgatgtt caggggcgtc gcttccggca cattgaagca ggtatggaat
     5641 gtctgaccgc aggcgtcggt cacggtcact gcatggcaga tgcccgccgc caatccggca
     5701 gccatactgc cgctttgccc cgatgcccag ctaaaaacat aaggcgcacg tccgccttcg
     5761 gccgtcactg tgagggcgcc gttcatttga ccgggacagg ccggctgcga agatgaaagc
     5821 gccagactga gcgccgaaac aggcatcacc gggtcggtgc agtgttctac ggtttgcatg
     5881 cagcgcagtt ccgtcacctc aaaacaatag ctttggccgg cggcgatgcc cgtcatgcac
     5941 aactcgtcgg agccgtcggg ccaggaaacc tgaagggcaa aatccccgtt cgtattcagg
     6001 cacagcatgc cgtcagactg ccccggacat ggcggcgtga tgctgagcag gtcggatacg
     6061 agttcttcta aagacttaca aaaatcaata cactcctccg ttgtacaacc attcgcgtct
     6121 ttggctgtca ggcagtaacg tccgggcggc acgcctgaaa tttgagaagc tgtagaggtg
     6181 aaaccatcgg ggccttccca ttgccatgaa atgggggcca gaccgcccag agccgttgcg
     6241 gaggccgagt tgttgttgtg attcacactc aggctcagcg ccatacaatc ccttatcagc
     6301 gtatgcacat tgtcttcgtt gttgcctgag acggccggag accatacaca gtcgggcatg
     6361 aaacggggca gcgcagcaca gtcgctcatc tggttgctcg ccgtgcgcaa atcaaacagg
     6421 gcattgccgc gcaggtccat gcccgaaaac acaaatgttt ctgctatgcg gccattttgt
     6481 actatacttg ccgcttggtt ggtggttaaa gtaaacaccc aggcattgcc gcccgcaggc
     6541 aagcctgtta aatttaaaac attcattgag aacatagaag gcgccggcat catcggttcg
     6601 ctgaactccg cgtcgaagcg caggctgcgc tgcccgtttt tgatttgggc aaacaccgtc
     6661 tggggcatat ccgtctcatt gggcaagaag aagaggcaat cgccggcggc ttccacccat
     6721 tcggcatcat aaatgagcgt ttcctctgcc gtagtggtat gtatggcggt gatgcgcagg
     6781 cggcgcaggt agggcaatgc gtcctgcggg gtcatgggca tggtattgaa cccggcctga
     6841 tcgaagcagc cgttgtcgtc ggtgacggtg aggatgtaaa tgccgcccgt cagattggag
     6901 aggtcttcgg agaccgcgcc gttgttccat tcgtattcaa aaggcgctgt gccgttggcg
     6961 atgttgattt gtatgcttcc gtcggcattg ttggcacagg acgacaactg cacctgcccc
     7021 gaaatatcgg gcggggtatg ctgggctacg gtaaaactcg ccagcgcggt gcagcccgtt
     7081 gcgtcggtga cggtcacttt atacactccg gcattgaccg ggccgaggtc ttccagcggc
     7141 catccgttgt tccactgaaa agcataaggc gcctgccctc cgctgacgga aaggtcaaca
     7201 ccgccttgcg attgttgaaa acattctccg aat
//
LOCUS       DAORLF010000240         7204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5614_length_7204_cov_1.878305, whole genome shotgun sequence.
ACCESSION   DAORLF010000240 DAORLF010000000
VERSION     DAORLF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_7204_cov_1.878305"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..>1041)
                     /locus_tag="PK198_17155"
     CDS             complement(314..>1041)
                     /locus_tag="PK198_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF40525.1"
                     /translation="LAATINLPTGVNQQMNVTPTYFEFISSDPTIATVSSTGLVSVVG
                     GPGSATITATVGGVTAQGSLRIQSVGNFQPAPVPTRPASRVISIFSDAYPNVPVNYYN
                     GYWQPWQTTVSNDFAVSGNNVLNYTIFNFVGIEFSSPTVNANAMTHLHMDVFIPGPVV
                     PGRELRVIVVDFGPDGGFQGGDDTRHSTTFAPPTLASGRWISIDIPFSSMTGLTRRAN
                     LAQIILEGGDGGSIYVDNIYFYN"
     assembly_gap    1042..1110
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1111..1704)
                     /locus_tag="PK198_17160"
     CDS             complement(<1111..1704)
                     /locus_tag="PK198_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40526.1"
                     /translation="MKNIFFRFIGAALLLTAWACERDVADLEPAAYPTNPNVFIDGFS
                     AGLNYGAFGSSVPTAFQVDNEVTYNQSRASMRFEVPDVNDPRGAYAGGVFFTSTGRDL
                     SGYDALSFWAKASQPANIDVLGFGNDFGESRYQVSLSGVGVNTNWKKYIIPIPDPSKL
                     KTERGMFFYSEGPENDRGYTFWIDEVKFEKLGAVAHPT"
     gene            complement(1777..4923)
                     /locus_tag="PK198_17165"
     CDS             complement(1777..4923)
                     /locus_tag="PK198_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRF40527.1"
                     /translation="METITKRFILFLTISVFSIVVRAQADKVVVSYGDNGIRLTVNGK
                     ELMVNGMNWDYFPIGTNYSYSLWDQSDAAIRQALDAEMGLLKNMGVNTIRVYTGIPQK
                     WIEYIYRTHGIYTMLNHSFGRYGLTIGGAWAPNTEYSDPRVRELLLKEVQTLAAEYRN
                     TPGLLLYLLGNENNYGLFWEGAETENIPMADRKSTRRARAMYKLFNEAAVLMKAADNT
                     HPVAMCNGDLLFLDIIAQECKEVDIFGTNVYRGVSFGDLFERVKKEYGKPVLFTEFGA
                     DAFNVIANGEDQSAQAHFLKENWREIYENAAGLGKSDNSIGGFTFQFSDGWWKYGQTS
                     NLDVHDSHASWSNGGYFNDYTAGENNMNEEWFGICAKGPTNAQGLYQLYPRVAYYVLK
                     DVHQLNPYAPGTTVSSVQGFFGGVQISDALLRARGDKAALEGEKNKFLRLSRFGAAFT
                     TFNTGGSLITTPKDPDPQNPVYPNQLGFDHMQSLFIGVEANPAPNMRANIELNVLGNV
                     AQNPIDQIFYENRGRPVQFAGLNGPITVESNNRVQIYKASYTWDHKLFNLNGFYRTGH
                     YHWGYEGDFFGLYPEANYGPQIDIYNGIAPFGFEVEGKKKLEGLKLAFGPQLWWGANP
                     AVLVKYSRDLAKFKWTAVFHEDLEQLGLTESSFAIPQPKTRRLTLHTARKFGKLGVDL
                     GGIWGGQPLNGREFQLVRGKEGEYTVYQDEIKNTDNWGGKIKLTYTGGTFNWYAQSAV
                     MGLVAGGGADLTQTFTGWRLKDSGSGNQYNFLTGFTVTMGNLQIGPNFLWQKPIEGPI
                     PAGLAAPARPRNILDDPFVVRGNREQMAGEILFTYDPTPGTWMYTWDSDRSEDAKFAL
                     SAGFVYRHYPTTQDAAIGIFPDGRTTFAFPGGAPAQDLWEVNARLVSKVNRDFGFIAN
                     IYGGTAQANGSDARTISRTGLDFRMVYKAVKLQSFARVNDWGPYDYHRDFNLTFPMQL
                     MADLSTSIAKPDWFDLPNTRIGLRGTWRALDKFSPRYAPAYTLDGAGNWVPDPNAVGF
                     GNGNEWEIRTYILINVGN"
     assembly_gap    5312..5321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5356..6576)
                     /locus_tag="PK198_17170"
     CDS             complement(5356..6576)
                     /locus_tag="PK198_17170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40528.1"
                     /translation="MSSSALIKEFKFVAITPPNGANIYVDNIYFAKVNCTDVTPPMLN
                     CTNITVTFNGQNSIPLTASQLATATDDFGTPILSINPTVITCQQVGQSVPVTVTARDA
                     TGNTATCISTVTVAGLPCGWSQQPNGVNCANGNSIAFNAATGVYTATSTGCSYGPPFS
                     SDATAFAQRTLCGNGSITARVTGINPLAGGWAGIVMREGNAAGAKKAQLMTNLGSDHR
                     REFRTTTNGAAQPQQFPSLSRYWLRITRVGNQFTMFVSSNGTNWFPAGSQNIAMSNCI
                     EMGLVATNNTSNSTVTATFSNVSFSGSTSGLGTGGGSMEQSAHSIEAPHSFEVYPNPT
                     GGELNLDLTQYIGRSVRIEMYSLEGKLLQFSELDEVQTTLKSLDLSRFQNGMYLVKVK
                     SAGLPDAVRRVVKQ"
     assembly_gap    6832..6868
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6869..7185)
                     /locus_tag="PK198_17175"
     CDS             complement(<6869..7185)
                     /locus_tag="PK198_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40529.1"
                     /translation="MGTTNIWCGTFAIPAGETVRYSFFYAAAAGAGGFENLTGLTCAN
                     NGRRTYTAVAGSPETLTFAWESCSSTPVCPPPPAPTVTAPVPTCAQANVASVYSDTYT
                     NIAG"
BASE COUNT         1609 a         1840 c         1863 g         1776 t
ORIGIN      
        1 tgttgagatt catctgcgtt gcaacccatc agaacgatga gcggcaatag gagaagcaat
       61 ttggtattca tttttctgat tttttagaag ttgttggacg gatgaaaaat tcgagttgca
      121 agcctctgaa cggcattctt ttgagcataa tcattcctgc gattcttggc ttacaaccct
      181 actaatcaag catctgaaga ggttatacct tgcgccgcca ggttttgtgc atagccttcg
      241 gcgcaaggtg ttggttagca gtgctcggta tgacaaaagc ggcatcgcac ctggctgtgc
      301 gaggtataaa ttctcaattg taaaagtaga tattatcaac ataaatgctt cctccatcac
      361 cgccttccag gataatctgt gccaggttgg ctcttctggt caggcctgtc atgctggaga
      421 agggaatatc aatggaaatc catctgcctg aagccaatgt aggcggcgcg aacgtggtcg
      481 aatgccgggt gtcgtcccct ccctggaatc ctccatccgg gccaaaatca acaacgatga
      541 ctctaagctc tcttcctggc actaccgggc ctggtatgaa gacatccatg tggaggtgag
      601 tcattgcgtt ggcattgacg gtggggctgc tgaattcaat gcccacaaag ttgaatatgg
      661 tatagttcaa aacattattt ccgctgacag caaaatcgtt agaaactgtt gtctgccagg
      721 gttgccaata accattgtag tagttcaccg gaacgttggg atacgcatcg ctgaagatcg
      781 aaatcaccct cgacgcagga cgcgtaggaa cgggggccgg ctgaaaattg cctactgatt
      841 gtatcctcaa agaaccttgc gccgtaacgc cccccaccgt ggcagtaatg gtggccgagc
      901 cggggccgcc tacgactgat accagacccg tactgctcac cgtggcaatg gtcgggtctg
      961 acgaaatgaa ttcaaagtag gtcggagtga cgttcatttg ctgattcaca ccggtgggca
     1021 gattgatggt agctgccagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgtcgggtgc gcaacggcgc cgagtttttc
     1141 aaatttcacc tcgtcaatcc aaaaggtgta accccgatca ttttcagggc cttcagaata
     1201 gaagaacata ccgcgctcgg tcttgagttt cgaggggtcg gggatgggaa taatatattt
     1261 tttccaattg gtattcaccc ctacgcccga aagagacacc tgatatcttg actcgccgaa
     1321 gtcgttgccg aagcccagta cgtcaatgtt cgccggctga gaagctttcg cccaaaaaga
     1381 gagcgcatcg tagcccgata aatcacggcc cgtactcgtg aaaaatacgc cgcctgcata
     1441 cgcgcctcgc gggtcgttca catcgggtac ttcaaatcgc atggaggccc gcgattggtt
     1501 gtaggtcact tcattgtcca cctgaaaagc ggtgggtact gagcttccaa aagctccata
     1561 gttcaagccg gcactgaatc cgtcaataaa tacattggga ttggtagggt aagcggctgg
     1621 ttccaaatcg gcaacgtcac gctcgcaggc ccatgccgtc aggagcaatg cagctccgat
     1681 aaagcggaaa aagatatttt tcatgatatt ttgtctttaa gttttgattg taaaacctct
     1741 cctcgcagct cgcggctcat agctcgcagc caatagttaa tttcctacgt tgatgagtat
     1801 gtaagtcctg atctcccact cgttaccgtt accaaagccc accgcgttgg gatcgggcac
     1861 ccagtttccg gcgccgtcga gggtataggc aggcgcatat ctcggcgaga atttgtccaa
     1921 agcccgccag gtaccacgca atccgatgcg ggtattcggc aggtcgaacc aatcgggctt
     1981 ggcaatgctg gtcgaaagat cagccatgag ctgcatcggg aaagtgaggt tgaaatcccg
     2041 gtgataatca taaggccccc agtcatttac gcgagcaaag gactgcaatt ttacagcctt
     2101 gtaaaccatg cgaaaatcca aaccggtgcg actgatggtg cgggcatcgc tgccgttggc
     2161 ctgtgccgtg ccgccatata tattggcgat gaagccgaaa tcgcggttca ctttcgatac
     2221 caatcgggca ttcacttccc agaggtcttg tgccggcgcg ccgcccggaa acgcgaaggt
     2281 ggtgcgtccg tcggggaaga taccaatggc ggcatcctgc gtagtgggat agtggcgata
     2341 tacgaagccg gcgctgaggg caaatttggc atcctcgctg cggtcgctgt cccaagtgta
     2401 catccaggta ccgggcgtgg ggtcataagt gaacaaaatt tcaccagcca tctgctcgcg
     2461 gttgccccgc accacgaagg ggtcgtcgag gatgttgcgc gggcgggcag gagcagccag
     2521 gccggcgggt atcggccctt cgatgggctt ctgccacagg aagttaggcc ctatttgaag
     2581 gttgcccatc gtcacggtga aacctgtgag gaaattgtac tggttgccgc tgccgctgtc
     2641 tttcagtcgc cagccggtaa aggtttgcgt cagatcggcg ccgccgccgg ctactaagcc
     2701 catcactgcc gactgtgcat accagttgaa ggtgccgcct gtgtaggtca acttgatttt
     2761 gccgccccag ttgtcggtat ttttgatctc atcctgatag acggtgtatt cgccttcttt
     2821 gccacgcacc aactgaaact cgcggccatt caaaggctgc ccgccccaga taccgcccag
     2881 gtcaacgccg agtttgccga acttccgggc agtgtgcagg gtgaggcggc gggttttggg
     2941 ttgcggaatg gcaaaggagc tttccgtgag gcccaactgt tccaagtctt catggaaaac
     3001 agccgtccat ttgaatttgg ccagatcgcg gctgtatttg accaacacgg ccgggttggc
     3061 gccccaccag agttgcgggc cgaaagccag cttcagtcct tccaatttct tcttgccttc
     3121 cacctcaaaa ccgaagggcg ctatgccgtt gtaaatatct atttgcggac cgtagttggc
     3181 ttctgggtac agaccgaaga aatcgccctc gtagccccag tgatagtgac cggtacgata
     3241 aaagccattg agattgaaca acttgtgatc ccaggtgtag cttgctttgt atatctgcac
     3301 ccggttgttg gattccaccg tgatcgggcc gttcagccct gcaaattgca ccgggcggcc
     3361 tctgttttca taaaaaatct ggtctatcgg gttctgcgcc acatttccca gcacgttcag
     3421 ttctatgttg gcccgcatgt tcggggcggg attggcttct actcctataa acagggactg
     3481 catgtgatcg aagcccaatt ggttgggata cacagggttt tgaggatcgg ggtctttggg
     3541 agtggtaatg aggctgccgc cggtattgaa cgtagtaaag gctgccccga agcggctcag
     3601 gcgcaaaaac ttattttttt caccctccaa cgcggccttg tcgccccgcg cccgcaacag
     3661 cgcatccgag atttgaacac ctccgaaaaa gccctgaacg ctgctcaccg tagtgccggg
     3721 tgcataagga ttgagttggt gaacgtcttt gagcacatag taggctacgc ggggatacaa
     3781 ttggtacaaa ccctgtgcat tggtcggccc tttggcacag atgccgaacc actcttcgtt
     3841 catgttgttc tcgccggccg tgtagtcgtt gaagtatccg ccgttcgacc aggaagcgtg
     3901 cgaatcatgc acatccaaat tggaggtttg gccatatttc caccagccgt cgctgaactg
     3961 gaaggtaaaa ccgccgatgg agttgtcgga cttgcccaga ccagctgcgt tttcgtaaat
     4021 ctcgcgccag ttttctttca aaaagtgagc ctgcgcactc tgatcttctc cattggcaat
     4081 aacgttgaac gcatccgcgc caaactcggt gaacaacacc ggcttgccgt actctttttt
     4141 gacgcgctca aacaggtcgc cgaacgaaac cccgcgatac acattggtac caaagatatc
     4201 cacctctttg cactcctgtg cgatgatgtc taaaaatagt aaatcgccgt tgcacatcgc
     4261 taccggatgt gtattgtcgg ccgctttcat cagcacggca gcctcgttga agagcttgta
     4321 catggcgcgc gcgcgccgtg tggacttgcg gtcggccatc ggaatgtttt ccgtttcggc
     4381 gccctcccaa aacaggccgt agttgttttc attacccaac aggtagagca acaaacccgg
     4441 cgtattgcgg tactctgccg ccaaggtttg cacctctttc agcagcagtt cgcgcacccg
     4501 cgggtcggaa tactccgtat tgggcgccca cgccccgcct atggtgaggc cgtacctgcc
     4561 gaaggagtgg ttgagcatgg tgtaaatgcc atgcgtgcga taaatgtact caatccattt
     4621 ttgcggaatg ccggtataaa cgcggatggt gttcacgccc atgtttttga gcagccccat
     4681 ctcggcatcc aacgcttgcc gaatagctgc atccgactga tcccacaaac tataggagta
     4741 attggtaccg atgggaaagt agtcccagtt cataccgtta accatcagtt ccttgccgtt
     4801 tacagtcagg cgaatgccat tatcgccgta ggacaccact accttgtcgg cctgtgcacg
     4861 tacaactatg gaaaaaactg aaatcgtaag gaacaggata aatcgtttag tgattgtttc
     4921 catctgaaat attttatttg gtaaaatagg ccggcgactc attctactga atcgtcgtgt
     4981 aaacttactg ccgtttatta caaaaaactg catatacatt atatatacac ggctgaaagg
     5041 cggggattca ttcaaattga aattgttcaa aatattgttt tacagatact tgtatgtaaa
     5101 ctcatactca gtcagagctg tttgtttttt ttcatgtata tgtatattgt ggtagtacaa
     5161 caggtgttcc atctcctttg gcgagaggat gcgatacctg tattttttcg tggtctctta
     5221 tcttacttta ccctgcttgt aaggatgtca attggagcaa taaaaaaaag acagccgaag
     5281 caatggctcg ctccggctgt ctcaccgtcg gnnnnnnnnn ncaacccttg tggttgccct
     5341 ccgttcaagc tattctcatt gcttcaccac ccgtcttacg gcatccggca gtccggcaga
     5401 tttcactttc accaagtaca tcccattctg gaacctgctc agatcgaggc ttttgagcgt
     5461 ggtttgcact tcgtccaatt cagagaattg caagagcttg ccttctaagc tgtacatctc
     5521 tatgcgcacg ctgcggccga tgtactgcgt caggtcgagg ttcaactcgc cccccgtcgg
     5581 gttcggatac acctcgaagc tgtgcggcgc ctctatgcta tgtgcgctct gctccatgct
     5641 gccgccgcct gttcccagcc cgctggtgct gccagagaag ctcacattcg agaacgtggc
     5701 cgtcaccgtg ctgttggagg tgttgttggt cgctaccaaa cccatttcga tgcaattgct
     5761 catcgcgatg ttttgcgagc cggccgggaa ccaattggtt ccgttggatg atacaaacat
     5821 agtgaactga ttccccacac gcgtgatgcg caaccagtag cggctcaggc tcgggaactg
     5881 ctgcggctgt gctgctccat tggtggttgt gcggaactcc cggcggtgat cgctgcctaa
     5941 gttcgtcatc aactgcgcct ttttcgcgcc tgctgcattg ccctcgcgca tcacgatgcc
     6001 cgcccagccg ccggccagcg gattgatacc agtgactctg gccgtgatac taccgttgcc
     6061 gcacagcgtg cgttgagcaa aggccgtggc atcgctgctg aatggcggcc cgtaggaaca
     6121 gcctgtactt gtggcggtgt aaacgcctgt agctgcattg aaggcaatgc tgttgccatt
     6181 ggcacagttc accccgttcg gttgctggct ccagccgcat ggcaggccgg ctactgtgac
     6241 agtgctgata caagtagctg tgttgccggt cgcatccctt gccgttaccg ttacaggcac
     6301 gctctggcct acctgttggc aggtgatgac agttggattg attgagagga tgggcgttcc
     6361 gaaatcgtca gttgcagtgg ccaattggct tgccgtgagc ggaatgctgt tctggccatt
     6421 gaatgtgacc gtaatgttgg tgcagtttag catgggaggc gttacatcgg tacaatttac
     6481 tttggcaaag taaatattgt ccacataaat attagcgcca ttgggaggag taatagctac
     6541 gaacttaaat tctttgatta aagcgcttga agacattcca agagccacat aatcagccaa
     6601 cggaatattt atactattcc atgaatttaa agttaatgac ctccttactg ccctttcgac
     6661 tcctgtagtt gcaactaaat aaatatcaat tgaactacag ttagccgacc aaatatctaa
     6721 acgtaaagtt tggaatgaat caatcctttg atggactccg agtgcaatac cctgataatt
     6781 catgttggga taatagcgca tttggttacc actaagtgtg actgtggttg cnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ggctatattt gtgtaggtat cactatagac
     6901 tgaagcaaca ttcgcttgcg cgcaggtggg cacaggcgct gttaccgttg gtgcaggggg
     6961 cggaggacaa acaggcgtgc tgctgcaaga ctcccacgca aacgtgagcg tttcagggct
     7021 gcctgccact gcggtataag tgcgcctgcc gttgttggca caggtcaagc ctgtcaggtt
     7081 ttcaaagccg cctgcaccgg cggcggcggc atagaagaag ctgtaccgga ctgtttctcc
     7141 tgcaggaata gcaaaggtgc cgcaccagat gttggtagta cccattctgg tcaaatttct
     7201 gaaa
//
LOCUS       DAORLF010000241         7110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5705_length_7110_cov_1.855989, whole genome shotgun sequence.
ACCESSION   DAORLF010000241 DAORLF010000000
VERSION     DAORLF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_7110_cov_1.855989"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..>364)
                     /locus_tag="PK198_17180"
     CDS             complement(110..>364)
                     /locus_tag="PK198_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40530.1"
                     /translation="VTDACGEPVTGLSASNFKVDPCIVGAGGALVNITSVAPVRIPGT
                     YIINVVPIRTETWKAGVYIFAVAVHSGTGQGLTLCSMLMD"
     assembly_gap    365..376
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    718..769
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<770..1386)
                     /locus_tag="PK198_17185"
     CDS             complement(<770..1386)
                     /locus_tag="PK198_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40531.1"
                     /translation="MMRAIVADADHASREALRGQVDAICPQVSVEGVANSAESAKQLM
                     DEIRPELVFIDEGLIEWPHAEQWSPKGQFFELILLTSQPAFRKEALQLDTTAYLFKPV
                     SDRELASAIHQSLHSIQLARDYRENREMLIKVIHHELPQEMISIPTMEGVEFLRLDEI
                     VRCEGLQGFTRIVIEEQESFLSSYNIGEFRKLLEPFGFFCPHKSHL"
     gene            1746..2330
                     /locus_tag="PK198_17190"
     CDS             1746..2330
                     /locus_tag="PK198_17190"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF40532.1"
                     /translation="MTITSLDQLNPDGTYTYADYLLWKFEERVELLRGKIRKIDSPSR
                     KHQEVVTGLTRLFANALWKNSCKVYVAPFDVRLTRTREDKDAVTVVQPDLCVICDPVK
                     LDARGCNGAPDLIVEILSPGNSRTEMKDKFELYQEAGVLEYWVVSPIEKTIQVWKLTE
                     QGTYIGLQPKVEGDVVTTPIVPKLEVDVSEVFMD"
     gene            2484..2981
                     /locus_tag="PK198_17195"
     CDS             2484..2981
                     /locus_tag="PK198_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40533.1"
                     /translation="MTSFRIRPRFRVEVEMKPEEIKTHFRERLEQPGAPCVAAFFPQH
                     VILRVPPEERHFWSPCLELSLEEEGDHTLIRGLYGPNPQVWTMFILIYGAIGVLALFI
                     TIIGVSRVMLDMSAPVLWALPVLGGAALLLYIFSQTGQKLGAEQTFTLHHFFEDTVHH
                     RAPVS"
     gene            3063..>3243
                     /locus_tag="PK198_17200"
     CDS             3063..>3243
                     /locus_tag="PK198_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40534.1"
                     /translation="MTPSDEAYNRDATAEDSSFNVFTELKAQYATLSDKSRSHAEKIE
                     AFENITDMEVGDTPLI"
     assembly_gap    3244..3318
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3319..>4130
                     /locus_tag="PK198_17205"
     CDS             <3319..>4130
                     /locus_tag="PK198_17205"
                     /inference="COORDINATES: protein motif:HMM:NF004996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF40535.1"
                     /translation="TQKDRIAFAQCMDALRRGYEGITLATCGNYGAACALAARIAGLR
                     CVIFLPENYHSKRLQEMTQLGAEIVRSPGDYEQAVVQSQQFAVQGDFYDANPGGNNTS
                     LQLKAYGEIAYEIYDELRDAPRIVAVPVSNGTVLAGIYKGFVSLFRRGKTSRIPMMVA
                     GSAHGKNPIVEAFLQNKTTCEDLLPQRIRETEVNEPLISWHAFDGDLSLAALRQTHGW
                     ARNVTDKRMLAVAKMLKDKEGLHTLPASTAGLAALLEAPKPEMVQNDRFVAV"
     assembly_gap    4131..4205
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4474..4632
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4633..5704
                     /locus_tag="PK198_17210"
     CDS             <4633..5704
                     /locus_tag="PK198_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF40536.1"
                     /translation="PDFDDPIQMAGGENNAFTNSDFTNFYETLPAENIETAFWLESDR
                     MLSLSFDPKVLEVQQKVVVEEFKETCLNQPYGDAWHLISDLAYREHPYRWPTIGLVPA
                     HIEEATLQDVRDFFFRYYRPNNAILTVAGKVKPELVKALAEKWFGDIPRGETPPRRLT
                     AEPPQQQLNRLVQRANVPVEALYLAFHMPGRLHPDYYAADLLSDVLCNGQSSRLFRRL
                     LKEEQLFSSIDCYVTGSVDPGLLIIEGKPAEGVSIETCRDAIWRELDLLKNERIGEHE
                     LEKIKNKVESNLIFSEISILTKASSLAFYEMLGDADMINTEGESYQNVSAEDIQRMAR
                     EMLTEENCNELVYKPLEVVEQE"
     assembly_gap    6069..6153
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6154..>7110)
                     /locus_tag="PK198_17215"
     CDS             complement(<6154..>7110)
                     /locus_tag="PK198_17215"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40537.1"
                     /translation="LGKTNRVVVLIDEYDKPINDYLTDYEMADTNRDVLGNFFGALKA
                     NDITDNLRFVFITGVSKFSKVTLFSELNNLTDITQHDEFGTLVGITQSELEGYFGEHS
                     EKLANKLGLTREAVLAKIKHWYNGYTWDGRNFLYNPFSLLGLFGSGSFKNFWFTSGTT
                     GWLARKLRDMAVNMANVEGMLVRESFFDKFEIRNLDPTVLLYQTGYLTIKEIIIDDAE
                     ATYRLDYPNYEVRSSLFDSLFQAYADTPVSEVGEIMYQIRNALTGHQLPEFISLLRGI
                     FADISNRLLKQYVEADSLKPWEAYYQTVIYLTLHLTGSQVAC"
BASE COUNT         1628 a         1758 c         1705 g         1561 t
ORIGIN      
        1 gcagagttga gcgttttttt tcgatgaaaa acaattgaaa cttcatcata ccccaatagc
       61 aacagggggc attcatggtc ataccccctg caaaacgaac ctgcaaatat taatccatca
      121 gcatggaaca gagcgtcaaa ccctggcctg tgccgctatg tacggccact gcgaagatat
      181 acacgcctgc tttccaggtt tccgtacgta tcggaactac gttgataatg taggtgccgg
      241 gaattctgac aggggccact gaggtaatgt tgaccaaggc tccgcccgct ccaacaatgc
      301 agggatctac tttgaaattg gaagcggaaa gacctgtaac cggctcgccg catgcgtcgg
      361 tgacnnnnnn nnnnnncaat atagctgggc tgtcggttgc cgggagcggc aacggtgtcc
      421 tcagagaggg cttcaatacg gaggagtgcc ataataggag gaattaaatg tgaaaaaatg
      481 aatacaacaa cgcctgttgc tgaacataag cggaaataag tattgacttc tttcgcctgt
      541 tttcaaagtt ggtgtcattc ctccaaaccc tcaacagtaa tcggagtatc ggcagggtta
      601 gttggatcat cggtaaaaaa gaaaaaccgt atgaaagcag ccatcttcac agtcgcctta
      661 cccgctccaa aaactccagc cgacgccgac gcgccaccgg cacgcaggag ccgtctcnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggtgggattt
      781 gtgggggcag aagaaaccaa aaggctccag cagtttgcga aactccccaa tattatatga
      841 actgaggaag ctctcctgtt cttctatgac aatgcgcgtg aacccctgca gcccctcgca
      901 gcggacaatc tcatccaatc tcagaaattc aacaccttcc atcgtgggaa tactgatcat
      961 ttcctgaggc aactcatggt gtatgacctt tataagcatt tctctgtttt ccctataatc
     1021 ccgagccaac tgtatgctgt gaagcgactg gtgaatagcc gacgccagtt cccgatccga
     1081 tacgggttta aaaagatatg cagtagtgtc caattggagc gcttctttgc gaaaagccgg
     1141 ttgggaggta agtaggataa gttcaaagaa ttgccctttg gggctccact gctccgcgtg
     1201 gggccattcg atgaggcctt catctataaa aactaactcg ggtcgaatct catccattag
     1261 ctgcttggcg ctttccgcag aattcgccac gccttcaacg gatacctgtg ggcagatggc
     1321 gtcgacttgt ccccgaagcg cttcccggga ggcatggtcg gcatcggcaa caatagcgcg
     1381 catcatggat atggagtttt gggtatgcag agaaaatctt caatttttac acaggtgagt
     1441 tacttacttt gaaattattt ggcaatgact tatatcagta catatcaaaa tttatcaaaa
     1501 tcgtctttca acgcctgcta ccggctttat ttttttcgac aggatttaca caggattcac
     1561 agaatggcat accgaaaatc ccgatccgga aatagcactg gcgacggtgg ggaggccgca
     1621 ctgtggaccg ggtgagatgg tcactttgag gtgaccgcct cacttcgcgg gcataaaatc
     1681 actgccgggc gttcggatcc atcaaaaaag cctatttttg caaatacaac gtcaatcttg
     1741 ctgccatgac tatcacatcg ctggatcagt tgaatcctga cggaacttat acctacgccg
     1801 attatctgct ttggaaattc gaagagcggg tagaactgct gcgcggcaaa attcgcaaaa
     1861 tagattcccc ttcgcgcaag caccaggaag tcgtcaccgg ccttacccgt ttgtttgcca
     1921 atgctttatg gaagaattca tgcaaggtat atgttgctcc cttcgatgtg cgcctcacgc
     1981 gtacccggga ggacaaagat gccgtcacgg tagtgcaacc tgatttgtgc gtcatttgtg
     2041 atccggtgaa attagatgct cgtggttgta atggcgcccc cgacctgatc gtagaaattc
     2101 tgtcgcccgg caacagccgc accgagatga aagacaagtt tgagctctat caggaagccg
     2161 gcgtgttgga atattgggtg gtttccccca tcgaaaagac gattcaagtg tggaaactca
     2221 ccgagcaggg tacttacatc ggcctccagc ctaaagtaga aggagatgtc gtcactacgc
     2281 ccattgtacc caagctcgaa gtggacgtat cggaggtgtt tatggattga ttctgacgtc
     2341 agccgcctgc tcattgtgct gaaccgcctc acttcgcgag ccaagcgaga caagctcctc
     2401 cccacagcaa tttttagaat aaaaaaacca gaaacggcga tttttgcgca aggacatttt
     2461 atcttggccg taaaaatcga aatatgacca gttttcgcat tcgcccccga ttcagggtgg
     2521 aagtagagat gaagccggaa gaaatcaaaa cgcatttcag ggagcgtttg gagcagcccg
     2581 gagcgccctg tgtggccgca ttttttccgc agcacgtcat tttgcgggtg ccgccggagg
     2641 aacggcattt ttggtcgccg tgtctggaac tgtcattgga agaagaaggc gatcacaccc
     2701 tgattcgcgg cttgtacggc cccaatccgc aggtctggac gatgttcatt ctgatatacg
     2761 gcgccatcgg cgtgctggcc ctgttcatta ccatcatcgg cgtttccaga gtcatgctcg
     2821 acatgagcgc gcccgttctg tgggccttgc cggtgttggg cggagcggct ttgttgttgt
     2881 acattttcag tcaaaccggc caaaaactcg gcgctgaaca aaccttcact ttgcatcatt
     2941 tctttgaaga tacggtgcat cacagagcgc ccgtgtccta attcagatca gcacaaattg
     3001 ccccgatgac gcaggagccg agattagcac atcgtcacat tagcacatta tcacatcatc
     3061 ctatgacgcc cagcgacgaa gcatacaacc gggacgcgac tgccgaagac agttcgttca
     3121 acgttttcac ggagctgaaa gcccaatacg caacgctgtc cgacaaaagc cggtctcatg
     3181 cagagaaaat tgaggctttt gaaaacatca ccgacatgga ggtgggcgat acgccgctca
     3241 ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnac tcagaaagac cgcatcgctt ttgcgcaatg tatggacgcg
     3361 ctgcggcggg gctacgaagg catcacgctg gccacctgcg gcaactacgg cgctgcctgc
     3421 gcattggccg cccgcattgc aggcttgcgc tgtgtgattt tcctgcccga aaactaccat
     3481 tccaagcgat tgcaggaaat gacccaactc ggcgcagaaa tagtccgctc gcccggcgat
     3541 tatgagcagg cggtggttca atcgcagcaa tttgccgtac aaggcgattt ttacgatgcc
     3601 aatcccggcg gcaacaacac ctcgcttcaa ctcaaagcct acggcgaaat tgcctacgaa
     3661 atctacgacg aactgcgcga tgcgccgcgc attgtggcgg ttccggtatc caacggtacg
     3721 gtgctggcag gtatttacaa aggtttcgtc agtctttttc gccggggcaa aacctcccgc
     3781 attcccatga tggtagccgg ctcggcgcat ggcaaaaacc ccattgtgga ggcattttta
     3841 caaaataaaa ccacctgcga agacctgctt ccccagcgca tccgcgagac ggaggtgaac
     3901 gagccgctca tcagttggca cgctttcgac ggcgacctct cgctggcagc cctgcgccaa
     3961 acccacggct gggcgcgcaa cgtgaccgac aaacggatgc tggcggtggc caaaatgctg
     4021 aaagacaaag agggcctgca cacgctgccc gcctccaccg ccggattggc cgccctgctc
     4081 gaagcgccaa agcctgaaat ggtgcaaaac gaccgctttg tggcggtttt nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnncaatg ccataccaaa gccgccgccg aaactaccaa agccgaaggg aacgcaatgc
     4261 ccgcaccggg cgtgtcgccc gcatattggt cgtgtctgtt ttgttgtttg cggccatttt
     4321 tgcctttatg aaccgctatt ggctctacgg ttggttgaaa acctattttt attagcgtta
     4381 tctttgccgc acatcagcac aacttgcccc gatgacgcaa gaaatcggga tcagcacata
     4441 attatatgat cgaatactca agatttgaac tcannnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnccccgatt tcgacgaccc catccaaatg gccggcggag agaacaatgc
     4681 gtttaccaac agtgatttca ccaactttta cgaaacgctg cccgccgaaa acatagagac
     4741 ggctttctgg ttggagagcg accgcatgct cagcctcagc ttcgatccca aagtgctgga
     4801 agtacagcaa aaagtggtgg tggaggagtt taaggagacc tgcctgaacc aaccctacgg
     4861 cgatgcctgg cacctcattt ccgacctcgc ataccgagag cacccgtacc gctggcccac
     4921 catcggactt gtgcctgcac acattgagga agctaccttg caggatgtgc gcgatttttt
     4981 cttccggtac taccggccca acaacgccat cctcaccgtg gccggcaagg taaaacctga
     5041 attagtgaaa gcgctggcag agaagtggtt tggagacatt ccgcgcggag agacgccgcc
     5101 tcgtcgattg actgccgagc cgccccaaca gcaactcaac cgtctggtac aacgcgccaa
     5161 tgtgccggtg gaggcgctct acttggcctt ccacatgccg gggcgcctgc atcccgacta
     5221 ttacgctgcc gacttgttgt cggatgtgct ttgcaacggc cagtcttcac gcctgtttcg
     5281 ccgcctgctc aaagaggagc aattgttctc ctccattgac tgctacgtta ccggttcggt
     5341 ggaccctggc ctgctcatca tcgagggcaa acctgccgag ggcgtttcca tcgaaacttg
     5401 ccgggatgca atctggcggg aattggattt gctcaaaaac gagcgcatcg gcgagcacga
     5461 attggaaaaa atcaaaaaca aggtagagag caacctcatt ttttcagaaa tcagcatcct
     5521 caccaaagcc tcttcgctgg cgttttatga aatgctgggc gatgccgata tgatcaatac
     5581 cgaaggggag agttaccaaa atgtttctgc cgaagacatt caacgcatgg cccgcgagat
     5641 gttgacggag gagaactgca atgagttggt ttataagccg ttggaggttg ttgagcaaga
     5701 gtagaggtct gtttttttta gaaaaatcgc agttgcttaa catatcccca aaagctacaa
     5761 gccgcaagct gttagctcgt ttttactgaa gcaatcagat tttcaatatg gggaaaattt
     5821 gagaatgtcc ttttgaaaat aaactatttt ccttttgcaa atgcaagcta aaggctatta
     5881 gctattggca atgggatacg ctcaatcaat acgaaaagct acaccttctc ctcctcccaa
     5941 tccccaatat tgcgcgttgc gggatcgaag cccacggcca gcagcgtcac gggtttgccc
     6001 cggtgcaggt atttttcggg gtagcccttg tccttgatct gttgcatggc ctcggcggct
     6061 ttgcccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcaggc cacctgactg cccgtgagat
     6181 ggagtgtcag gtagatcacc gtttggtaat acgcttccca gggtttgagg ctgtcggctt
     6241 ccacatactg tttgagcagg cggttggaaa tatcggcgaa aatgccccgc agcaggctga
     6301 tgaactccgg caattggtgt cccgtcaggg cattgcggat ttggtacatg atttcgccca
     6361 cctcgctcac aggcgtatcg gcgtaagcct gaaagagact gtcgaacagc gaactgcgca
     6421 cctcgtagtt gggatagtcc aggcggtagg ttgcctccgc atcgtctatg atgatctctt
     6481 tgatggtcag gtagccggtt tggtacagca gcacggtagg gtccaagttt cggatttcaa
     6541 acttatcgaa aaaagactcc cgcaccagca taccctccac gttggccata ttgacggcca
     6601 tgtcgcgcag tttgcgggcc agccatccgg tagtgccgct ggtgaaccag aaatttttga
     6661 aactgccgct gccgaacaat cccaacagcg agaacggatt gtatagaaag tttcgcccgt
     6721 cccaggtgta gccgttgtac cagtgcttga ttttggctaa aacggcctct ctggtgagac
     6781 ccaacttatt ggccaatttt tcactatgtt cgccgaaata cccttccagc tctgactgcg
     6841 tgatgcccac caaggtgccg aactcgtcgt gctgcgttat gtccgtcagg ttgttgagtt
     6901 ccgaaaaaag ggtcactttg gaaaacttgc tcaccccggt aatgaagacg aagcgcaggt
     6961 tgtcggtgat gtcgttggcc ttgagcgcgc cgaaaaaatt ccccagcaca tcgcggttgg
     7021 tgtcggccat ttcgtaatcg gtcaggtagt cgttgatggg cttgtcgtat tcatctataa
     7081 ggacgacgac gcgattggtt ttgcctaatt
//
LOCUS       DAORLF010000242         7097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5718_length_7097_cov_2.125533, whole genome shotgun sequence.
ACCESSION   DAORLF010000242 DAORLF010000000
VERSION     DAORLF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_7097_cov_2.125533"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK198_17220"
     CDS             <1..205
                     /locus_tag="PK198_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40538.1"
                     /translation="FGQHIILADRIVLSLSAEINASPLQLNLTPSYDDSEKDNQTIFK
                     EAAAARMRFHSLIMFKMGAGYLF"
     gene            231..1115
                     /locus_tag="PK198_17225"
     CDS             231..1115
                     /locus_tag="PK198_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40539.1"
                     /translation="MKPILFIAILAGLLSGCSQYEVQYEGPYEDRLNPVQPAIPAFEI
                     LTVENGKIVLYHRNLSQTKTLNNLPANIAKAAVNHSHDRIAYQVAGQNVVIVDSTGAP
                     VDIVPNSAAAVWFDWHANNQTLCLLSGTSLSFWGPALALPSTNLASLFPAGSSDRTMS
                     CAIVTGNGSVVAAYRYYAGFSQGYQSRITLLPPSGAPVHRVVGSFDETRWLRTDRDGQ
                     RVLGGAVVSGSIFSTWNIDLSAQTITQTGDQPFAAPGPSPEQTAFWKNGSLSVYHSGL
                     DQWFGVQTGQTGFTTLDW"
     gene            1154..3391
                     /locus_tag="PK198_17230"
     CDS             1154..3391
                     /locus_tag="PK198_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF40540.1"
                     /translation="MKTVFCIAWCFLFQVFALHAQSDYAPLECQGTIPKEFISSSTSK
                     YRASVKNASRQTQKQQEAKARSRFALESNFNLDNLLRSGRVLFNDEVSNYLADVTHLL
                     LDVQPLSRKQDIRIYALRSSAVNAFATERGEIFVTLGLIAQLENEAQLAYIIAHELAH
                     VEKKHVINFYLEAEKLERSKGRSVLGRATTDENMLRMATYSKEHEHEADRLGLERILA
                     SPYRAATLPVVFDVLRYSYLPFDDLPFDRAWLQSEHYRLPDALWLEAVKPIEGEADEE
                     DDSRNSHPGLQSRRQAMLHTLANHAHAPEKTDYAVSAQRFQRVRTLARYELPMLYLHT
                     DQHANAIYTAGLLLQEQPDNLALKKCIAKALYLHAKLRNSKNYEYKGNYQEVEGQSQQ
                     LHYLLEKIPDVEATVLAWHFVWSLHRAYPADTEITTLANDLFFEFAREHNSFAELRND
                     LPLALADIAPADTAQYAPEGRSKYDRIREQKTSADAHADYWRYAFSGQLNDEAFVKYR
                     ESAEKRRDQWKENERYTNSEKGKKEFRRNRKQGLSLGISKVVVINPFYLKLDERKGEA
                     VQYIDTEKGREHLHSLIKSVSTVSGLKTTLLDVHDLKENQSETFNDIRYLNEWFSEQA
                     RHFDLSLTPGNEQERIDAIAEKYGTDYFLWTGIISLREKHTAADLAYLPLSLLLFPPA
                     APLAIHNLVKPKHEMLLFSILYDVRTGRRQIVKFDTFNQRDSDAVVKAHLYDAFTQIG
                     TKARQ"
     assembly_gap    3470..3509
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3538..>5213)
                     /locus_tag="PK198_17235"
     CDS             complement(3538..>5213)
                     /locus_tag="PK198_17235"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40541.1"
                     /translation="MIGLDSNNSMRILQFLLALFTCLPLFAQVDAPMVQINRELESAY
                     RDSVARHEDFGAILHYQPHRCRSRYGRFGWVDKYHKVVIPFDYQEFPEYLSDFNPAKK
                     EGKHGAVDAKGKTIVPFKYSSLSPYYAQRLVICRDGSYQITVFDFEGRVVIPEEKLYN
                     IWLFNDSTLAVQDFSKKDVRTYDLRGRPIKTWAYSHIYHFSSGRILTSRDTLMGLGTA
                     TTHGMLDPGGKVLVPLEYTSIEWEKGDWARVANYKTKTGGLFQISTQTLHPDKYYKVL
                     PPDPLGNFTFWEGASWEQTKAGLMDAHFKVIFPPAYNDIRFLDEKGHYALHAGRNNRG
                     YLRGVGNARGEVVADTTLLGFEPVIHEKKVGQNARGQWIIVYDTLPFFVFEDARSGQK
                     GLWHRQAGQLRAAAFDRFEAVTDTTFIQEKGDTSFLFHIDGRPLAGPYYRIWRGRIDD
                     YLLVRPNWNDEYILLTQDGRFIRRLEGEPRRLADGIYTMGRYGNKTSGYGKVKEYGFF
                     DRALNPITGLVFDAEPKSMDGLNTQQKIRVLNRPHPTGYGRWVGYMREG"
     assembly_gap    5214..5239
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5240..6785)
                     /locus_tag="PK198_17240"
     CDS             complement(<5240..6785)
                     /locus_tag="PK198_17240"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40542.1"
                     /translation="MKLSTTLRIFATVCLLQLATVLPAQQPDVLEELPRTIQKTTKPP
                     APAGYAYNDYGQIVTAQQAYWSELGSHYTRKFSQNGKNGMKAQDGTVLIPAEYDDLHF
                     QYCGFMVASKEGKFGVINEANKVVIPFDYKRLELLYKPLAFQVPKGVPLEDLRLLATR
                     DDGQSGLLNGKGELVFPFRENRYVQVAYFYPAARTTQGYVTTELPDYQSAMRQTALIY
                     HQPDEKGIVAMDGKILLPFEYAAIDVSYGTDERPAEHPEWVQVCSADNACGIFDMRRR
                     AWMIEPKFYGGIALMQASPSLFVTQAETEPDEHGVTYTRYGAIDSTGRTVLPFEYMEF
                     GLSFLYQSRLHIWAQKMGKWGVVDIDNKVVMPFEHPKVIQHLKMERGPHFSLKDPAGE
                     LFGVLSLEGKLAVPLKYKWLGDQNGRLIIFVNEQMKCGLVNDQGREVLPGIYTDIQLL
                     RHGFFYINAESKLGLVDPSGKILAQPTYRGAYLENTLPAFSGDFKAKGIPEAEVVTVL
                     TREDGAA"
BASE COUNT         1741 a         1908 c         1730 g         1652 t
ORIGIN      
        1 gtttgggcag cacatcattc tcgccgaccg cattgtgctg agtttgtcgg cagagataaa
       61 cgcctctccg ctgcaactca accttactcc atcctacgac gattcggaaa aagacaatca
      121 aaccatcttt aaggaagccg ccgccgcgcg catgcgcttc cactcgctca ttatgttcaa
      181 aatgggggca ggataccttt tttaaatacc ctgataaaaa cacgctcaac atgaaaccaa
      241 tcttgtttat cgctattctg gcagggctgc tcagcggctg ttctcaatac gaggtccaat
      301 atgaaggccc ctacgaagac cgtctgaatc ctgtgcaacc ggccattcca gcctttgaaa
      361 tactgacggt agagaacggc aaaatcgtcc tttatcaccg aaatctgagc cagaccaaaa
      421 ccctcaacaa cctgcctgcc aacattgcga aagcggccgt caaccattcc cacgaccgca
      481 ttgcatatca ggtggcaggg caaaatgtcg tcatagtgga ctccacaggc gcacccgttg
      541 acatagtgcc caactccgcc gccgcagtgt ggtttgactg gcacgccaac aaccaaacgc
      601 tctgcctgct cagtggcact tcgctctcct tttgggggcc tgccctcgcc ttgccttcta
      661 ccaatttggc ctccctgttc cctgccggct cctccgaccg aaccatgtct tgcgccatag
      721 tgaccggcaa cggcagcgta gtggccgcat acaggtacta cgccggattt tcgcaggggt
      781 accagagccg catcaccctg ttgccgcctt ccggcgcgcc ggtgcatcgc gttgtcggca
      841 gtttcgacga aacgcgttgg ttgcgcacag atcgggacgg gcagcgcgtt ttgggtggcg
      901 cggtggtatc tggcagcatt tttagtacat ggaacatcga cctttccgcc caaacgatta
      961 ctcaaacagg cgatcagccc ttcgctgcgc cggggccctc gcccgaacag actgcatttt
     1021 ggaaaaacgg cagcctgagc gtgtatcatt ccggcctcga tcagtggttc ggggtacaaa
     1081 cagggcaaac cggcttcacc accttggact ggtgatctta aacatccttt tacctactca
     1141 aaaacatttc acaatgaaaa ccgtattttg catcgcttgg tgctttcttt ttcaggtatt
     1201 tgcattgcac gcacaaagcg actacgctcc tttagagtgt cagggcacaa ttcccaaaga
     1261 atttatatct tcctccacat ccaaataccg cgcatctgta aaaaacgcgt cgcgccaaac
     1321 acaaaagcag caagaggcca aagcccgcag ccggtttgca ctggaaagca atttcaatct
     1381 cgacaacctc ttgcgcagcg ggcgggtgtt gttcaatgat gaagtttcca attatctggc
     1441 cgatgtaact catttattat tggacgttca gccgttgtcg cgcaagcagg atattcgaat
     1501 atatgcactc cgcagcagcg ccgtgaacgc ctttgccaca gagcgcggag agatattcgt
     1561 cacgcttggc cttatcgccc aattggaaaa cgaagcacaa ttggcctaca tcattgcgca
     1621 cgaattggcg catgtagaga aaaaacacgt catcaatttt tatctcgaag ccgaaaaatt
     1681 ggagcgctcc aaagggcgta gcgtactggg gcgggctaca accgatgaaa acatgctcag
     1741 aatggccaca tattccaaag aacacgaaca tgaagccgac cgcctcggat tggaacgcat
     1801 actcgcgtct ccttaccgtg ctgccacgct tcctgtggtc tttgatgtgc tcagatacag
     1861 ttacctgccg tttgatgatc tgccgtttga tcgcgcgtgg ctgcaatcgg agcactaccg
     1921 cctgcccgat gccttgtggt tggaagcagt caaacccata gaaggcgaag ccgacgaaga
     1981 ggacgacagc cgaaactcgc accccggcct ccaatcgcgc agacaagcca tgctccatac
     2041 attggcgaac catgcccatg cccccgaaaa aacagactac gcggtgtctg ctcaacgctt
     2101 ccaacgcgtg cgtaccttag ctcgatacga actccccatg ctctacctgc ataccgacca
     2161 acacgcgaac gccatttata ccgccggcct gctgctgcaa gaacaacccg acaacctcgc
     2221 cttaaaaaag tgcattgcca aagccctgta tctgcatgcg aaactcagaa acagcaaaaa
     2281 ctatgagtat aagggcaatt atcaggaagt agagggccaa tcccagcaat tgcactacct
     2341 cctggagaaa attcccgacg tagaggctac cgttctggct tggcattttg tctggagcct
     2401 gcaccgcgca tatcccgccg atacagaaat aacaacgctg gcaaatgatc ttttttttga
     2461 atttgcaaga gaacataact cctttgccga actgcgaaac gacctgccct tggctctcgc
     2521 cgacatcgcc cctgccgaca ctgcccaata cgctccggag ggccgatcca aatacgaccg
     2581 cattcgcgag caaaaaacga gtgccgacgc tcacgccgac tactggcgat acgccttctc
     2641 cgggcaattg aacgacgagg cttttgtaaa gtaccgggaa agtgctgaaa agcgccgcga
     2701 ccaatggaaa gaaaacgagc gctatacaaa ctcagaaaaa ggcaaaaagg aattcaggcg
     2761 caacaggaaa caagggctga gcctgggtat ttccaaagtg gtcgtcatca atccgttcta
     2821 tctcaaactg gatgagcgaa aaggggaggc cgtacaatac attgacaccg aaaaaggacg
     2881 ggaacacctc cacagcctca tcaagtcggt gagtacagta tccggcctga aaaccacttt
     2941 gctggatgtg catgatttga aagaaaatca aagtgagacc ttcaatgaca tccggtatct
     3001 caacgaatgg ttctcagagc aggcaaggca ttttgacctc agtcttaccc caggcaatga
     3061 gcaggagcgc attgatgcca ttgccgaaaa atacggcacc gattatttcc tttggaccgg
     3121 catcatctcc ctgcgggaaa agcacactgc cgccgacctg gcctatctgc ctttgtcgct
     3181 gttgctcttc cctccggcgg cgccgctggc cattcacaac ctcgtgaagc ccaaacacga
     3241 aatgctgctc ttttccattc tgtacgatgt gcgcacaggc cgccgccaga tagtcaaatt
     3301 cgacaccttt aatcaacgcg attcagacgc cgtcgtcaag gcacacctgt atgatgcgtt
     3361 cacacaaata ggaacgaagg cccggcagta gtggcgctgc gcgcaaacaa aaacgaaagc
     3421 cgatgtcgga gggaactccg gcatcggctt tttcgattgg gttagcctcn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atcatctatc aatatgagcg tctcgtctca
     3541 tccttcccgc atgtacccta cccagcgccc atagcctgtt ggatgcggcc ggttcagtac
     3601 gcgtattttt tgctgggtat tgagtccgtc catagatttc ggttcagcat cgaagacaag
     3661 tccggtgatg gggttcaaag ccctgtcaaa gaaaccatac tcttttacct tgccgtaccc
     3721 ggatgtttta ttgccataac gacccatagt gtaaatgccg tcagccagac ggcggggttc
     3781 gccttcgagc cggcggatga agcggccatc ctgagtgagc aggatgtatt cgtcgttcca
     3841 attcgggcgg acaaggagat aatcatctat ccgaccacgc cagatgcgat agtaagggcc
     3901 ggctaatgga cgcccgtcaa tatgaaacag aaaggaagta tcgccttttt cctgaataaa
     3961 agtggtatct gtaactgcct caaatctgtc gaatgctgcg gcccgaagct gcccggcttg
     4021 ccgatgccac aagccttttt ggccagaccg ggcgtcttca aaaacaaaga acggcaaggt
     4081 atcatatact atgatccact gcccgcgcgc gttttgcccc actttttttt cgtgtatcac
     4141 cggctcgaat cctaacaacg tagtgtctgc gaccacctcg ccacgggcgt tgcccacacc
     4201 gcgcaggtag cctcggttgt tgcgcccggc gtgcagggca taatgtcctt tttcgtccaa
     4261 aaaacgaata tcgttgtagg ctggaggaaa aataactttg aaatgagcat ccatcagacc
     4321 ggccttggtc tgctcccacg aggcgccttc ccaaaaggta aaatttccga gcgggtccgg
     4381 tgggaggact ttgtagtatt tgtcggggtg aagtgtctgt gtgctgatct ggaacagacc
     4441 gcctgttttg gttttgtaat tggcgactct cgcccaatcg cctttttccc attcaatgct
     4501 ggtatattcc aaaggcacga gcacctttcc gccggggtcc agcattccat gcgtggtagc
     4561 cgtgcccaac cccatcaggg tgtcccggct tgtaagaatg cggccggaag aaaaatggta
     4621 gatgtgcgaa tatgcccagg ttttaatggg ccgcccccga agatcatagg ttctgacgtc
     4681 tttcttgctg aagtcctgca cggccagcgt actgtcgttg aagagccaga tattgtataa
     4741 cttttcttcg ggaataacga cccggccctc aaagtcgaaa acggttattt ggtacgatcc
     4801 atccctgcaa atgaccaatc gctgcgcgta ataaggagat aaagacgagt atttgaaagg
     4861 aacaatggtc tttcctttag catcaacagc accatgtttt ccttcttttt tagctggatt
     4921 gaagtctgat aaatactcag gaaactcctg ataatcaaat ggaatgacca ctttatggta
     4981 tttgtccacc caaccgaaac gcccatatcg gcttcggcaa cgatgcggtt ggtaatgcag
     5041 gatagcgccg aaatcttcgt gtcttgcgac tgaatcgcgg taggcggatt ccaattcccg
     5101 gtttatctgt accatagggg catccacctg tgcgaagagg ggcaaacagg taaaaagagc
     5161 aagaagaaac tgtaagatgc gcatagagtt gtttgagtcc agaccgatca tttnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnc agccgccccg tcctcgcggg tcagcaccgt cacgacctcc
     5281 gcttcgggaa tgcctttggc tttgaagtcg ccggaaaaag caggcagcgt gttctccaga
     5341 taggcgcctc ggtaagtcgg ttgcgccaat attttccccg aaggatcaac aagacccagc
     5401 ttgctttctg cattgatgta aaaaaagcca tgcctgagca actgaatatc tgtataaatg
     5461 cccggcaaga cttcccggcc ctgatcgttg accaatccgc acttcatctg ctcgtttacg
     5521 aaaatgatca gacgcccgtt ttgatcgccc aaccatttgt atttcaacgg taccgccaat
     5581 ttgccctcca aagaaagcac accaaagagc tctcccgcgg ggtctttgag tgaaaaatgc
     5641 ggcccgcgct ccattttcaa gtgttggatg actttaggat gttcaaaggg catcaccact
     5701 ttattgtcta tgtccaccac gccccatttg cccatcttct gcgcccagat atgcaaccgg
     5761 ctctgataga ggaatgaaag gccgaattcc atatattcaa aaggtagtac cgtgcgtccg
     5821 gtgctgtcaa tggctccgta tcgggtatag gtcacgccat gctcatccgg ctccgtttcg
     5881 gcttgggtga caaaaagcga cggcgaggcc tgcatcagcg ctatgccccc ataaaacttg
     5941 ggttcgatca tccaggcacg gcggcgcata tcaaaaatac cgcacgcgtt gtctgcgctg
     6001 caaacctgta cccattctgg atgttctgct gggcgctcgt cggttccata cgagacatct
     6061 atggctgcat attcgaaggg caggaggatt ttgccgtcca ttgccactat gcctttttca
     6121 tccggttggt ggtagatcag tgccgtttgg cgcatggcag actggtagtc aggcagttcg
     6181 gtggtcacat agccctgcgt ggtacgcgca gcagggtaga aataggccac ttggacgtag
     6241 cggttttcac ggaaaggaaa aacaagttca ccttttccgt tgaggaggcc cgactgcccg
     6301 tcgtcgcggg tggcgagcag gcgaaggtct tctaagggaa cgcctttggg cacctgaaat
     6361 gctaatggtt tgtaaagcaa ctcaagccgt ttatagtcga aaggaatgac caccttgttg
     6421 gcctcgttga tgacaccgaa tttcccctct ttcgatgcca ccatgaagcc gcagtattgg
     6481 aagtgcaggt cgtcgtattc ggcggggatg agtacggttc cgtcctgagc cttcatgccg
     6541 tttttgccat tctgggaaaa tttacgggta tagtggctgc ccaactcgct ccaatacgcc
     6601 tgctgagccg taacgatctg gccgtagtcg ttgtaggcgt aaccggcagg ggcgggcggc
     6661 tttgttgttt tttgaatagt ccggggtagt tcttccaata cgtcgggttg ctgggcggga
     6721 agcacggtgg caagttggag caggcatacc gtggcaaaga tgcgcagggt tgttgatagt
     6781 ttcatagcgt gttattggtt tgctcgaata attcagactg caaatgtaaa attaaagtac
     6841 tcacggacgc tgcctgattt ttgttttgat atccattggt ttctgttcag aagaccttca
     6901 ggatttaacc aacatgtttt gggccacacc acgccaattc accgaattta gccaaataaa
     6961 gagaattgat tcactgaatt aacgttaatt cggtgaattt gatttatatt tgcggcagta
     7021 gcccgaccgt aaaccatcgg ctgccaatgt tatgaaagta ctaaaaagaa gtttgtacga
     7081 ccaattatcg tctaaat
//
LOCUS       DAORLF010000243         7093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5724_length_7093_cov_2.482666, whole genome shotgun sequence.
ACCESSION   DAORLF010000243 DAORLF010000000
VERSION     DAORLF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5724_length_7093_cov_2.482666"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1187
                     /locus_tag="PK198_17245"
     CDS             87..1187
                     /locus_tag="PK198_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40543.1"
                     /translation="MNAHIIAFTFSLLVVLSPALQASDYQGGTPTTIFETLLREDALD
                     ITIETDLGELINNRRRDDYQPATMTYKSQNGVSVRHNIEVMPRGKFRRRICDFPPVKI
                     KFSKKELTAAGLKKHNNLKLSTHCMDDKEAGNYNVFKEYLAYKLYNALTPNSFRVQLV
                     RVTYVDAAGQLSKVKRYGFLIEDDEELAERMGGSLCECLNAGSDMVAAYDETMMSVYN
                     YMIGNEDWSLSMVRNIKMVETAGGSKVLTIAYDFDFSGLVNASYALPSSDYEQRHIKD
                     RDYLGFEADAATMQNVLQRLREKKSELYRIISDFKLLPAEQRLEMRDYLESFYQIIQT
                     LPADSRQLPSWRFGAAAPVNAGTQGASETTGR"
     gene            1331..>1536
                     /locus_tag="PK198_17250"
     CDS             1331..>1536
                     /locus_tag="PK198_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40544.1"
                     /translation="MKHFLSLAAAGLLGAVLALAGSRLLFPVNESLANLEQPAARQAK
                     WMNTAPGAATSAPFDFTAASERAMP"
     assembly_gap    1537..1641
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1642..>1911
                     /locus_tag="PK198_17255"
     CDS             <1642..>1911
                     /locus_tag="PK198_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF40545.1"
                     /translation="FFLFGNPFGGPKKGTGSGVIYTSDGYIITNNHVVDFADTIEVTL
                     YDNRKFSATLVGTDPKTDLAVLKIEAGGLPILQLADSDQAKVGQWV"
     assembly_gap    1912..1921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1922..2514
                     /locus_tag="PK198_17260"
     CDS             <1922..2514
                     /locus_tag="PK198_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF40546.1"
                     /translation="VGNPFDLTSTVTAGIISAKGRNIKILRDNDAIEAFIQTDAAVNP
                     GNSGGALVDDQGRLLGINTAIATQTGSFAGYSFAIPSNLMRRIVEDLMEHGSFKRVYL
                     GVNIYDLNSDRAKELRIDISQGVVVESTVPGSSADKAGLRAKDVITRIDGRQVKNMPD
                     LQEIVSRSRAGDVLRMSVVRNGEELTIPVAMLAADE"
     gene            2511..2774
                     /locus_tag="PK198_17265"
     CDS             2511..2774
                     /locus_tag="PK198_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40547.1"
                     /translation="MSRNAALYRTLTIGQETKALARPFLCAKQSSQRIDLFKVGFSFC
                     FDAPVPIFGTGVFVFGPPLKRFLFDLTLEQALDEVQKNSIFMP"
     gene            3083..3844
                     /locus_tag="PK198_17270"
     CDS             3083..3844
                     /locus_tag="PK198_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF40548.1"
                     /translation="MLRTFLLSILFLNFAALTAAQTGGEIEYEITRQISPEMMRRFGG
                     GSEDSPMPSVITLNQAMRFAGNMATWTMPRPGMQMAGMMPPGAQGQMRRFMPFDSKDY
                     VDFAGGKFLHYLRATSGEDTTTWYLNEEPFEKPADWKVDKKTKKILGYECKKATATLR
                     DATYTIWYTTDIKGLHFSPINGLFPPDGVVLSIENDEQSFVAKKVDLKAAVPATEVTP
                     EIKDAKPITAEEMRDMRRKAGERMRAQFQQQMPRQ"
     gene            3998..6874
                     /locus_tag="PK198_17275"
     CDS             3998..6874
                     /locus_tag="PK198_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40549.1"
                     /translation="MRVTFIALFISCLSLSAAMGQSAGNGTIKGFLRSKPDNQPLPFS
                     AVMLQNPKDSSVAYSAVSNDKGFFQITGIADGEYRFVATYLGYTPLERSISISRGART
                     MDFGVLDMEEAEITLNQVTVKEDRIPVTVKQDTLEFDASAYKLRDNAVVEDILKRLPG
                     VEVGADGSIKAMGENVSEITVDGQPFFGSDPKLATRNLPADIVSKIQIHEKKSDQAQF
                     TGIDDGNTKKAINITLKKDKNEGVFGRITTGGGQTAGNDLGARFSLNGNVNRFRGKER
                     ISLLGSGNNVNDLGFSFSDLMTSFGGGGQSGDSGGGLSDLLGGGGGGMRMMQGGRGGL
                     SGALTGAGTGGITTAYAGGLNYRNEWGGGKLKFNSSYFGSQSNTVNDEERLRQTILEN
                     GSFSKLDEGHSENERSNHRLNMNLEYQIDSSRSIIFRPTLTFSKQNALSDNVYESVNF
                     EDVLLNDGSTLTRSDNTRPALSGNLLYRQRLRKAGRTISLNLTGSYNDQDTEQLNQSD
                     NRFYMPGGALLSDSSINQLSFQQSINQRLGARVAYTEPLGKNKFLELNYDFNNNFQNS
                     DRVTYDFNEVTGRYDVVNDVFSNNFENTFTTHQGGFNYRYQKLKYDYTLGMSVQESQI
                     SSESPEQQSTFSRSFLNFFPVAQFNYNFSQSKRLRINYRGNARNPSVSQLQPVPDVSN
                     PQSIRLGNPELSPEISHNINLNFNNFNMTSMRSMFLSLRSTLTNNRIVNATSITGRGQ
                     QTIIPVNTDGAWNASAFAAVGMPLVKKIKLNVNFNANFNYDHYANLFNGAENITNSLT
                     TGGGLRFTLTPNEKFDASLGANVSYNKAEQSIQAQLNSDFINYTASADFNWTLPLNFV
                     IGSDARLTANRGGSSTYNQEAILWGAYVEKRFLKNNSGALRLQGFDLLNQNINVNRTV
                     GDGFIEDLSTSILRQYFLLSFTYKFGKFAGQNMQMPPGMRMMRF"
BASE COUNT         1753 a         1994 c         1727 g         1504 t
ORIGIN      
        1 cgaattcgtc cttgtatgta tcgaataaat gcgccgctca gccgctccaa cgcacacctt
       61 tgtgccattc aaaaaaaatg cacaccatga atgcccatat tattgccttc acgttttctc
      121 tcctcgttgt tttatcacct gcattgcaag catctgacta tcagggcgga acgcctacca
      181 ctatctttga aaccctccta cgggaagacg cattggatat tacgatagag accgatttgg
      241 gagaactcat caacaaccgc cgccgcgacg actaccaacc ggccacgatg acatacaaat
      301 cacagaacgg cgtttcggtt cgccacaaca ttgaggtgat gcctcgtggg aagttccgtc
      361 gccgtatatg cgatttcccg ccggtcaaaa taaaattctc caaaaaagag cttactgccg
      421 ccggactgaa gaagcacaac aacctcaagc tctctacgca ctgcatggac gacaaagagg
      481 cgggcaacta caatgtgttc aaggaatatc tggcgtacaa gctttataac gcgctcactc
      541 cgaacagctt tcgagtgcaa ttggtccgcg tcacctatgt ggatgctgcc ggccagttga
      601 gcaaagtgaa gcgctacggt tttttgattg aagacgacga agaactggca gaacgcatgg
      661 gcggcagctt gtgcgaatgc ctcaatgccg gctcggacat ggtagccgcc tatgacgaaa
      721 ccatgatgtc ggtttacaac tacatgatcg gcaatgaaga ctggagtctg agcatggtgc
      781 gcaatataaa aatggtggaa acggccggcg gctctaaggt gttgaccatt gcctacgact
      841 tcgatttttc cggcttggtg aacgcgtcct acgccctgcc cagctccgac tacgaacagc
      901 gccacatcaa agaccgggat tatctggggt ttgaagctga tgccgcaacg atgcaaaacg
      961 tactccagcg cctgcgggag aaaaaaagcg aactgtatcg aatcatatcc gattttaaac
     1021 tcttgcccgc cgaacaacga ttggaaatgc gagattactt agagtcgttt taccaaatca
     1081 ttcaaacctt gcccgccgac agccggcaat tgccgagttg gcgattcgga gctgccgctc
     1141 ccgtgaatgc aggaacgcaa ggtgcaagcg aaacgaccgg gagataagca gcagcgcctt
     1201 caagtcaggt cgtaatttat ggttgcgatc tgaccaaagc tataccaagc ccttttgagg
     1261 ctatttttca cacttctgta taatccaggc ggcgatttgc gtgttgtaat ctccaaaaaa
     1321 cacatcacca atgaagcatt tcctttcttt ggcggcagcc ggactgctcg gcgccgtgct
     1381 ggccctggcg ggttcacgcc tgttgtttcc ggttaatgaa tccctcgcca acctcgaaca
     1441 accagccgcc cggcaagcca agtggatgaa cacggcgccc ggcgcggcga cttcggcccc
     1501 cttcgatttc acggcggctt ccgagcgcgc catgccnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nttttttctg ttcggcaatc ctttcggcgg ccccaaaaaa
     1681 ggcaccggat cgggtgtgat ctacacctcc gacggctata tcatcaccaa caaccatgtg
     1741 gtggactttg ccgacaccat tgaagtgaca ctctacgaca accgcaaatt ttccgccacc
     1801 ctcgtgggaa cagaccccaa aaccgatctg gccgtcctga aaattgaggc cggcggtctg
     1861 ccgatcctcc aactcgccga ttccgatcag gccaaagtgg gccaatgggt gnnnnnnnnn
     1921 nctgtgggca atccctttga cctcacctcc acggtgaccg ccggcatcat cagcgccaaa
     1981 ggccgcaaca tcaagatatt gcgagacaac gacgctattg aggcattcat tcaaaccgac
     2041 gctgccgtca atcccggcaa cagcggaggc gctttggtgg acgatcaggg ccgactgctg
     2101 ggcatcaaca ccgctattgc cacgcagacg gggtccttcg ccggctactc ctttgccatt
     2161 ccatccaacc tcatgcgccg catcgtggaa gacctcatgg agcacggcag tttcaagcgc
     2221 gtttacctcg gcgtcaacat ctatgacctc aacagcgacc gggccaaaga actgcgcata
     2281 gacatcagcc agggcgtggt ggtagagagc accgtgccgg gcagttcggc agacaaagca
     2341 gggttgcgcg ccaaagacgt catcacccgc attgacgggc ggcaggtaaa gaatatgcct
     2401 gatcttcagg aaattgtgag cagatcaaga gccggcgatg tgttgcgaat gtcggtagtg
     2461 cgcaatggcg aggagctgac cattcccgtg gccatgctgg ccgccgacga atgagtcgca
     2521 acgctgccct ttacaggact ttaacaattg ggcaggaaac taaagctctt gcaaggccgt
     2581 ttttatgtgc aaagcaatca tctcaaagaa tagacttgtt taaagttggt ttttcgtttt
     2641 gttttgatgc gcctgttcct attttcggaa cgggcgtttt tgtttttggc ccgccgctaa
     2701 aacgctttct attcgatctg accttagagc aggctcttga cgaagtgcaa aagaactcta
     2761 tctttatgcc gtaatcgcgc cgcctgataa gatttcactc ggaaggcgag gcgcccaaac
     2821 agtatggcag gttcgatgat gatgagtatt ttgatgatga ctgggaggaa gaggaataaa
     2881 acgacttttg atctctggtt atcggagtgt cttcctacat caatcacccc taccttggtg
     2941 gtattatcat caatcaagac gaaggctgca accttctccc cgtccctctg tttctttcgc
     3001 ttaacacaaa ttaacggtaa acaggagtgc ttaacgcata ctcctgcgac ctttgcatcg
     3061 gaaattttaa aaccatacaa ccatgttgag aacatttctt ttgagcatac tttttctgaa
     3121 ttttgctgcc ctgacagcgg cgcagaccgg aggcgagatc gaatatgaaa tcacccgaca
     3181 gatcagtccc gaaatgatgc gtcgcttcgg gggcggcagc gaagactcgc ccatgccttc
     3241 cgtcatcacc ctcaatcaag ccatgcgttt tgccggcaat atggccacct ggaccatgcc
     3301 ccggcccggt atgcagatgg ccggcatgat gcctccgggc gctcagggcc agatgcgccg
     3361 cttcatgcct ttcgacagca aagattatgt ggatttcgca ggcggcaaat tcctgcacta
     3421 cctccgcgcc acctccggcg aagacactac cacctggtac ctcaacgagg aaccgtttga
     3481 aaaaccggcc gactggaaag tggacaaaaa gaccaagaaa atattgggct acgagtgcaa
     3541 aaaagccacc gccaccttgc gcgatgccac ttacaccatc tggtacacta cagatatcaa
     3601 aggcttgcat tttagtccga tcaatggcct gttcccgccc gatggcgtgg tgctctccat
     3661 cgaaaacgat gagcagtctt tcgtcgctaa aaaagtggat ctgaaagcgg cagtacccgc
     3721 cacggaagta acgccggaaa tcaaagatgc caagcccatc accgccgagg aaatgcgcga
     3781 catgcgccgc aaagccggcg aaagaatgcg agcgcagttt cagcagcaga tgccacgtca
     3841 gtagttcggt aggcggtcag cagtaggcgg taggcggcgc ttccagaaac acggaacgcg
     3901 aaacaaaaaa acattcaacg ctcccttatg ctcgctccct ttttggggtg ggtgtttaat
     3961 tccccctttg gggactaggg agccaaacac cctcctcatg agagtcacat ttattgccct
     4021 gttcattagt tgtttgtcgc tgagtgcggc gatgggccaa agtgccggca acggtaccat
     4081 caaaggtttt ctgcgcagca aacccgacaa ccagccgctg cctttctcgg cagtcatgct
     4141 gcaaaatccg aaagattcct cggtagccta ttctgccgtt tccaacgaca aaggcttttt
     4201 ccagatcacc ggcatagccg acggcgagta ccgcttcgtg gccacttatt tgggttatac
     4261 gcccttagag cgcagcatca gcatctcgcg gggcgcccgt acgatggatt tcggcgtgtt
     4321 ggacatggaa gaggcagaaa tcaccctcaa ccaggtgacc gtgaaagaag accgaattcc
     4381 ggtaacggtg aaacaagata cgctggaatt tgacgcctcg gcctacaagc tccgcgacaa
     4441 cgccgtggtg gaagacatcc tcaagcgcct gcccggcgtg gaagtcggcg ccgatggttc
     4501 catcaaggcg atgggggaaa atgtcagtga aatcaccgtg gacgggcaac ccttcttcgg
     4561 cagtgacccc aagctggcca cccgcaacct gcccgccgac atcgtgtcca aaattcagat
     4621 acacgaaaaa aaatcagacc aggcacagtt caccggcata gacgacggca atactaaaaa
     4681 agccatcaac atcacgctga aaaaagacaa aaatgaaggc gtctttgggc gcatcaccac
     4741 cggcggcggc caaactgccg gcaatgacct cggtgcgcgc ttctcgctca acggcaacgt
     4801 gaaccgcttt cgcggaaaag agcgcatctc tctgctgggc agcggcaaca acgtcaacga
     4861 cttgggcttt tctttctccg atctgatgac ttctttcggc ggcggcggcc agtccggaga
     4921 tagtggtggc gggctttctg acctgctcgg cggcggcgga ggaggaatgc ggatgatgca
     4981 aggaggacgc ggcggcctca gcggcgcttt gaccggagcc ggaaccggcg gcatcactac
     5041 ggcttatgca ggcggcctca actaccgcaa cgaatggggc ggcggcaaac tcaagttcaa
     5101 cagctcctac ttcggcagcc aatccaacac cgtgaacgac gaagagcgcc tgcgccaaac
     5161 catcctcgaa aatggcagct tcagcaaatt ggacgaaggc cactccgaaa acgagcgctc
     5221 caaccaccgt ctcaacatga atctggagta tcagatagac agctcgcggt ccatcatttt
     5281 cagacccaca ctgaccttca gcaagcaaaa tgccttgtcg gacaatgtgt atgaatcggt
     5341 caactttgaa gatgtactcc tcaacgacgg cagcaccctc acccgttccg acaatacccg
     5401 gcccgccctc agcggcaacc tgctctatcg ccagcgtttg cgcaaagcag gccgcaccat
     5461 ctccctcaat cttaccggct cctacaacga tcaggacaca gaacaactca accaatccga
     5521 caaccgcttc tacatgcccg gcggcgcttt gctcagcgat tcttccatca accagctttc
     5581 gttccagcaa agcatcaatc agcggctcgg cgcccgcgtg gcctacaccg aacctttggg
     5641 caaaaacaag tttctggaat tgaactacga tttcaacaat aatttccaga attccgaccg
     5701 cgtcacttac gacttcaacg aggttaccgg ccgctacgat gtagtgaacg atgtgttttc
     5761 caacaatttt gaaaacacct ttaccacgca tcagggcggc ttcaactacc gctaccaaaa
     5821 gctcaaatac gactacacct tgggcatgag cgtgcaggag agccaaatca gcagcgaatc
     5881 gccggagcaa cagagtactt tctcccgctc attcctcaat ttctttccgg tggcgcagtt
     5941 caactacaat ttctcgcaaa gcaagcgcct gcgcatcaac taccgcggca atgcccgcaa
     6001 tccgagcgtg tcgcaattgc agcccgttcc cgacgtgagc aatccgcaaa gcattcgtct
     6061 gggcaatccc gaactcagcc cggagatttc tcacaacatc aacctgaatt tcaacaactt
     6121 caacatgacc tccatgcgga gcatgttcct ctctttgcgc agtacgctca ccaacaaccg
     6181 catcgttaac gctaccagca tcaccgggcg cggccagcaa accatcattc cggtgaacac
     6241 cgacggcgcc tggaatgcaa gcgcctttgc agcagtgggc atgccgttgg taaaaaagat
     6301 caagttgaat gtgaatttca atgccaattt taactacgat cactacgcca acctcttcaa
     6361 cggagcggaa aacatcacca actcgctgac caccggcggc ggcttgcggt ttacgctcac
     6421 gcccaatgag aagttcgatg cttcgctggg cgccaatgtg agctacaaca aagccgagca
     6481 atctattcag gcgcagctca actccgattt catcaactac accgcttccg ctgatttcaa
     6541 ctggacgctt ccgctgaatt tcgttatcgg ttccgatgcc cgccttacgg ccaatcgggg
     6601 cggttcgagt acttacaatc aggaggccat cctgtggggc gcttatgtag agaaacgttt
     6661 cctgaaaaat aattccggag cgttgcgttt gcaaggtttc gacctgctga accagaacat
     6721 caacgtgaat cgcacggtag gcgacggctt catcgaagac ctgagtacca gcatcctgcg
     6781 gcagtatttt ctgctgagtt tcacttataa gtttggcaag tttgccggcc agaacatgca
     6841 gatgcctccg ggtatgcgca tgatgaggtt ctgaggattt tagccgcttc aacaaaacaa
     6901 caaaaaggct tatgagtacg aaggcgctcg taagcctttt ttttattggg aaaatgggcg
     6961 ggcttcgcgg gctgttttcc tctgtgtgta taacagattg cacaaaatca ggcgcggttt
     7021 tcaggccgta cacaatttga caaatcttcg agatttgtca aattttcgct ccgccacccg
     7081 tcgaagcctg tct
//
LOCUS       DAORLF010000244         7085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5731_length_7085_cov_1.745946, whole genome shotgun sequence.
ACCESSION   DAORLF010000244 DAORLF010000000
VERSION     DAORLF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_7085_cov_1.745946"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /locus_tag="PK198_17280"
     CDS             complement(<1..>1578)
                     /locus_tag="PK198_17280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HRF40550.1"
                     /translation="DPVPIVDAIRKGLNVQTHIIAFIPAREIPKTSSGKIMRHKAKEM
                     WEEGQLNIISQFSGALEAEHLHEETHGESSPFEELKVKYGLKGNESFTLGNAGLESID
                     LAVLMHDIKELLKDKGADALAKQIDLRLIQEITISELFELVQQFENASALAVIRLKKM
                     ITRLQKEHTEMEQKMMLGDVHLTFEPETIVYPDSYHATDVLLTGGTGFFGPFLLKGIL
                     EQTDQRVYVLIRATSEEQGMERLIAAYNSVEPMTPEFERMFRSRIVPVCGDLGQRHLG
                     LTREKWNFLANTVQEIYHNGATVNYLFNYDKMRNVNVLGTNEILKLAFDGIPKVFNHI
                     STTFIFGWAVRDVLYESTTNDDINLLDFGYSQTKWVSEEIVKDAMRRGLKGRIFRPAL
                     ITPSINGGGHNFDISIRLLAFMINNSVGVDTHNQVSFSPADVAAHNIIAISNMPESVN
                     RTFHVTRDNYNNMMDITDIITRLTGKQFDQFKLKDFVPVILERCTKDDLLFPLLDFLV
                     RSVENISSMEFKLYDSG"
     assembly_gap    1579..1692
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1693..>1920)
                     /locus_tag="PK198_17285"
     CDS             complement(<1693..>1920)
                     /locus_tag="PK198_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF40551.1"
                     /translation="WITGQSKCLGYWNKPELTKERFQASIVGENSNANNYMRTGDMGF
                     IHQDELYICGRAKDMIIIRGLNYYPQDIEKIV"
     assembly_gap    1921..1967
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1968..3153)
                     /locus_tag="PK198_17290"
     CDS             complement(<1968..3153)
                     /locus_tag="PK198_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF40552.1"
                     /translation="MTSIFERLDHWAQESPDKLMYCFLDISGNETEKYTYGEFIYRTN
                     AIAGYLHQEHGIVAGDRLLMAYPPGLEMICAFFACIRLGAIPVPAYPPTSHGFESSLN
                     KMTYIAKDCQAKAILTSRDYFWTMKLNISRNNAETFFGSSYMSKLDWINTEDFRDEHR
                     KGFPAGHSDIMFLQYTSGSTSDPKGVMVTHQNILHNCDLATDFVPHAVTWLPQYHDMG
                     LIGYYIFVALKGGTTYGFAPIDFIQRPALWLESISKYRANASSAPNFAFEYCLQPGKI
                     SEKTLENLDLSSLDYLMTAAEPVIPHTFFRFKEFFMQYGLRPDSYFAAFGLAENTLAV
                     TNYGRTALTLDRAAFKKHKVKIMPAPDENIPYTEVMSCGPTQGDIIVRIVDPKTHHAL
                     KDD"
     gene            complement(3201..4295)
                     /locus_tag="PK198_17295"
     CDS             complement(3201..4295)
                     /locus_tag="PK198_17295"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRF40553.1"
                     /translation="MDTNATANREAFKKVLFERLNKYFEGGRSKKGDWEMTAKVIFAI
                     SWWIITYVLFYVNDWTYTQFFGLYVLQGLGQIFMFLNIAHDANHSAVSNKRFLNKSLS
                     YVLDACGISSYMWRIMHNIGHHSVMNVHGEDEGIHAHGIFRFSPHAPWKPYHRYQHIY
                     GFFLYFITSLDFLFVKDLEYLFLNNNKHIEGMKHPLREWITIIGSKILYLIYMIVLPV
                     TILGFSWPQIIIAFIVTHFIMGLAAALIIQTAHLLDVNEFPKTSHDYDYLDHILATTT
                     DYATRSRIANFICGGLNHHVAHHIFPQVAHTHYPKLTKIVRETAAEFGVEYRENKYMY
                     QAITQHFRLLRLLGLPPKGAVQKGPLAAHA"
     gene            4412..5515
                     /gene="lpxK"
                     /locus_tag="PK198_17300"
     CDS             4412..5515
                     /gene="lpxK"
                     /locus_tag="PK198_17300"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRF40554.1"
                     /translation="MPKICRSFARMLQQWIIRILLWPFSILYGLGATLHEKLYRWGLL
                     KAVSFNLPVISVGNLTVGGAGKSPHIEYLVRLLKEHLEVGVLSRGYHRKSVGFKEVLP
                     QMSVEESGDEPLQFKRKFPDVLVAVSESRSLGVPRMLMKRDDLQVVLLDDAFQHRAIQ
                     PGLNILLTEHDHPFTRDLLLPAGRLREWPSAYRRADIIVVTKCPADPAEVQREALLRE
                     IAPLPHQRVYFSYYQYRQPYYLLDNRYVTTLEDDWEVLLISAIANVDYLTQYLKQQVG
                     YIKIMEFEDHRYFSKSDISNLKLTFDNMEAQKKIILTTEKDATRLDQHRQFFTEHRLP
                     VFVLPVEVRFHFEDAGAPSFDDDVKNFLLNFKA"
     assembly_gap    5581..5594
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5595..>5915
                     /locus_tag="PK198_17305"
     CDS             <5595..>5915
                     /locus_tag="PK198_17305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF40555.1"
                     /translation="FYPHTASIDRHFLHHKGPAILIVNHPNTLLDALFGALSSPRYTF
                     FLANYSLFKNPVAGWLLNQLYCIPIQRYQDTNGRPLQNEDSFARADAHLAAGGQLLVA
                     PEGA"
     assembly_gap    5916..5940
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5941..>6233
                     /locus_tag="PK198_17310"
     CDS             <5941..>6233
                     /locus_tag="PK198_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40556.1"
                     /translation="KTGAARIAFSMASKSGFQSGLVFLPVGLTYERPHRFGGSVTVQA
                     GPPVAVDDFADAWAADPVQAVRELTQHLELKMQQLAVHTTDEAEDKLLHYAEI"
     assembly_gap    6234..6258
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6259..6846
                     /locus_tag="PK198_17315"
     CDS             <6259..6846
                     /locus_tag="PK198_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40557.1"
                     /translation="PLPQKEEYLRTRRLLDGFRGLQQTDAPGWERFSRDVRAYFFRLH
                     ALGLQDHAVAAPSLQFVWGHIAALWLTLPVFVLGWALNALPFHLPGFIADRAGAVPEY
                     KATWKYVGGLLFFTIWYVILYQLFRTQFSVLWSWGLLLLTPALGWAAWRYYQWALHTW
                     QSWKAWNVFRKKPELRQEWQEFRAALLQETKGMVI"
     gene            complement(6858..>7085)
                     /locus_tag="PK198_17320"
     CDS             complement(6858..>7085)
                     /locus_tag="PK198_17320"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRF40558.1"
                     /translation="KRTIEEKMDFYLDQNLNELEKELHQKLSHYPVSQSIYLKGSLDQ
                     LEIQNAWLAPEAIRVQIGLRGRIEVFVTGLN"
BASE COUNT         1479 a         1770 c         1929 g         1682 t
ORIGIN      
        1 tgccgctgtc gtagagcttg aactccatcg aggagatgtt ttccaccgag cgcacgagga
       61 agtcgagcag cggaaacagc aggtcgtctt tggtgcagcg ttcgaggatg accggcacaa
      121 agtctttgag tttgaactgg tcgaactgct tgccggtgag ccgcgtaatg atgtcggtga
      181 tgtccatcat gttgttgtag ttgtcgcggg tcacatggaa ggtgcggttc acactttccg
      241 gcatgttgga gatggcgatg atgttgtggg ctgccacgtc ggccggcgag aagctgacct
      301 ggttgtgcgt gtccacgcct acgctgttgt tgatcataaa ggccaacaaa cggatggaaa
      361 tgtcgaaatt gtgaccgccg ccgttgatgg agggcgtgat gagcgccggg cggaatatgc
      421 ggcctttcaa accgcgccgc atagcgtctt tcacaatctc ttccgacacc cacttggttt
      481 ggctgtagcc gaagtcgagc aggttgatgt cgtcgttggt ggtggattcg tacagcacgt
      541 cgcgcaccgc ccatccgaaa atgaaggtgg tggaaatgtg gttgaacact ttaggaatgc
      601 cgtcgaaagc cagcttgagg atttcgttgg tgcccaaaac gttcacattg cgcatcttgt
      661 cgtagttgaa caggtagttc accgtagcgc cgttgtggta aatttcctgc acggtattgg
      721 ctaagaaatt ccacttctcg cgggtgaggc cgaggtggcg ctgccccaaa tcgccgcaaa
      781 cgggaacgat gcgactgcgg aacatgcgct caaattccgg cgtcatgggt tctaccgaat
      841 tgtaggccgc gatgaggcgc tccatgccct gctcttcgct cgtggcgcga atgagcacat
      901 acacccgctg atcggtttgt tccaggatgc ctttgagcag gaacggcccg aagaagccgg
      961 tgccgccggt gagcagcacg tcggtggcgt ggtagctgtc gggatagacg attgtttcgg
     1021 gttcaaaagt gagatgcacg tcgccgagca tcattttttg ctccatttcg gtgtgctcct
     1081 tctggaggcg ggtaatcatc tttttcagtc ggatgaccgc cagcgcagag gcgttttcaa
     1141 actgctgcac caactcgaac agctcggaaa tggtgatttc ctgaatgagg cgcaggtcaa
     1201 tttgtttggc caatgcgtcg gcgcctttgt ccttgagcag ctctttgatg tcgtgcatca
     1261 acacggcgag gtcaatggat tccaagccgg cattgcccag cgtaaatgac tcgttgccct
     1321 tgagaccgta tttcactttc agttcctcaa aaggcgagct ttcgccatgc gtttcctcgt
     1381 gcaggtgttc ggcttcgagg gcgccggaga actgactgat gatgttgagt tggccttctt
     1441 cccacatttc tttggccttg tggcgcatga tcttgccaga actggttttg ggaatttccc
     1501 tcgccggaat gaaggcaatg atgtgggttt gaacgttcaa gcccttgcgg atggcatcca
     1561 cgatgggcac cggatcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nncactattt tttcgatgtc ctgcggatag taattgaggc cgcgaatgat
     1741 gatcatgtct ttggcgcgac cgcagatgta gagttcatcc tgatgaatga agcccatgtc
     1801 gccggtgcgc atgtagttgt tggcattgga gttttcgccc acgatgctgg cctgaaaacg
     1861 ctctttggtc agttcaggct tgttccaata tccgaggcat ttgctctggc cggtgatcca
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat cgtctttgag
     1981 ggcgtggtgg gttttggggt ccacaatgcg cacgatgatg tcgccctgag ttggtccgca
     2041 gctcatcact tctgtgtagg ggatgttttc gtcgggagcg ggcatgattt tgaccttgtg
     2101 ctttttgaaa gcggcccggt cgagggtgag ggcggtgcgt ccgtagttgg tcacagccag
     2161 cgtgttctcg gccaagccga aagctgcgaa gtaggaatcg gggcgcaggc cgtattgcat
     2221 aaagaactcc ttgaaccgga agaaggtgtg cggaatcacc ggttcggcag cggtcataag
     2281 gtaatcgagc gagctgagat cgaggttttc cagcgttttt tcagaaattt tgcccggctg
     2341 aagacagtat tcaaaagcaa aattgggcgc cgaagaggca ttggcccggt atttggaaat
     2401 actctccaac cacagtgccg gccgctgaat gaagtctatg ggtgcaaagc cgtaagtggt
     2461 accgcccttg agcgccacga aaatgtagta gccgatgagg cccatgtcgt ggtattgcgg
     2521 cagccaggta acggcgtgcg gtacaaagtc ggtagccaga tcgcagttgt gcaggatgtt
     2581 ctggtgcgtc accatgacac ctttgggatc gctggtggag ccggaggtat attggaggaa
     2641 catgatgtcg gaatgcccgg cgggaaatcc cttgcgatgc tcgtcgcgga agtcttctgt
     2701 attgatccag tctaatttcg acatgtagct gctgccgaag aaagtctccg cgttgttgcg
     2761 ggagatattg agtttcatcg tccagaagta gtcgcggctg gtgaggatgg ccttggcctg
     2821 acagtctttg gcgatgtagg tcattttatt gagcgaagac tcgaaaccgt gcgaagtagg
     2881 cgggtaggcc ggcacaggaa tggctccaag acgaatgcag gcaaagaatg cgcagatcat
     2941 ttccaatccc ggaggatagg ccatgagcag tcggtcgccg gccacgatgc cgtgctcctg
     3001 atgtaggtag ccggcaatgg cgttggtgcg ataaataaac tcgccgtaag tatatttttc
     3061 cgtttcattg ccggatatat ccaaaaagca atacatcagc ttgtcggggc tttcctgtgc
     3121 ccaatgatca agcctttcga agatagaagt cataagtgtg aaattatctg tttgtttaaa
     3181 gttggagaac tttcgcgggg ctatgcgtga gcagccagcg gccctttctg tacggcgcct
     3241 ttgggaggca gccccagtaa tcggagcagt cggaaatgct gagtaatagc ctgatacatg
     3301 tacttgtttt cgcggtactc cacgccgaat tcagcagcgg tttcgcgaac gatcttcgtc
     3361 agtttcggat agtgtgtgtg cgccacttgc ggaaaaatgt gatgcgcgac gtgatgattg
     3421 aggccgccgc agatgaagtt ggcaatgcgg ctgcgggtag catagtcggt cgttgtggcc
     3481 aggatgtgat ctaaataatc atagtcatgg ctggttttcg gaaactcgtt tacatcaagg
     3541 aggtgcgccg tttgaatgat aagcgcggca gccagcccca tgatgaaatg cgtgacgatg
     3601 aaagcgatga tgatttgcgg ccaggaaaag cccagtatcg tcaccggcaa tacgatcata
     3661 tatataaggt agaggatttt gctgccgatg atggtgatcc attcacgcaa ggggtgcttc
     3721 atgccctcaa tgtgcttgtt gttgttgagg aacaggtact ccaagtcctt cacaaacagg
     3781 aagtcaagcg aagtgatgaa gtacaaaaag aacccgtaga tgtgctgata gcggtggtag
     3841 ggtttccagg gtgcgtgggg agagaatcgg aaaatgccgt gcgcgtgtat gccttcgtct
     3901 tcgccatgaa cgttcatcac cgagtggtgg ccgatgttgt gcatgatgcg ccacatgtaa
     3961 gagctgatgc cgcaggcgtc gagcacatag ctcagcgatt tgttgagaaa gcgtttgttg
     4021 gacaccgcgc tgtggttggc atcgtgggcg atgttgagga acataaaaat ctgccccagc
     4081 ccttgtaaaa catacagccc aaagaattgg gtgtaggtcc agtcgttgac gtaaaacaaa
     4141 acataagtga tgatccacca ggaaatggcg aagatgactt tggccgtcat ttcccagtcg
     4201 ccttttttag aacgcccacc ttcaaaatac ttgttgaggc gttcaaacaa caccttttta
     4261 aaggcttccc gattcgctgt tgcgttcgta tccatgctgt ttaattttag ttgctacttt
     4321 gtcaggaaag cactgccgac tgtactgttg acaagcccct gcacgtttca gggcgcaaag
     4381 atagctaccc gccgtcaaac ggagtgcata catgccgaag atttgccgta gttttgcccg
     4441 catgttgcaa caatggatca ttcgcatatt actctggccg ttttccatac tgtacggatt
     4501 gggcgccaca ctgcacgaaa agctctaccg ttgggggctg ctcaaagcgg tcagtttcaa
     4561 ccttccggtc atatctgtgg gcaatctcac agtaggcggc gccggcaagt cgccgcacat
     4621 tgagtacctc gtcaggctgc tgaaagaaca cctcgaagtg ggcgtactct cgcggggata
     4681 tcaccgcaag tcggtaggtt ttaaagaagt gctgccgcaa atgagcgtag aagaatcggg
     4741 cgacgaaccc ctgcaattca aacgcaaatt ccccgacgta ctcgtggccg tcagcgaaag
     4801 ccgctccctc ggcgtgcccc gcatgttgat gaaacgcgac gacctgcaag tcgtactcct
     4861 cgacgacgcc ttccaacacc gcgccatcca accgggcctc aacatcctgc tcaccgaaca
     4921 cgaccacccc ttcacccgcg acctgctgct gcccgccggc cgcctccgcg aatggccctc
     4981 ggcttatcgc cgcgccgaca tcattgtggt caccaaatgc cccgccgatc ccgccgaagt
     5041 acaacgcgag gccctgctcc gcgaaatcgc acccctaccc caccagcggg tttatttttc
     5101 gtattaccaa taccggcaac cttactatct gcttgataat cgttatgtta ccactctgga
     5161 agacgactgg gaagtgttgc tcatcagcgc catagccaac gtggactacc tcacgcagta
     5221 cctcaaacag caggtcggct acatcaaaat catggaattt gaagaccacc gctatttcag
     5281 caaaagcgat atttccaact tgaaactgac cttcgacaac atggaggctc agaaaaaaat
     5341 catcctcacc accgaaaaag acgccacgcg attggaccag caccggcagt tttttaccga
     5401 gcatcgcctg cctgtttttg tgctgccggt ggaggtgcgt ttccattttg aagacgccgg
     5461 cgcccccagc ttcgacgacg atgtgaagaa tttcctgttg aatttcaaag cctgaccgcc
     5521 tgtgaatctg tttctgtctg ctctgtaccg cctgctcaaa gccattgtgg tcgtcaccct
     5581 nnnnnnnnnn nnnnttttta cccgcacacc gcctccattg accgccactt tttgcaccac
     5641 aaaggcccag ccattctcat tgtcaaccat cccaatacct tattggatgc gctgtttggc
     5701 gcgctcagtt cgccgcgcta caccttcttt ttggccaatt acagcctgtt caaaaaccct
     5761 gtggcgggtt ggctgctcaa tcagttgtac tgcatcccca tccaacgcta tcaggacacc
     5821 aatggccgcc cgctgcaaaa tgaggacagt tttgcccgcg ccgatgcaca tttggccgcc
     5881 ggcggccagt tgctcgtagc tcccgaaggc gccagnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 caaaaccggc gcggcccgca ttgccttcag tatggcctca aagagtgggt ttcagtcggg
     6001 gttggtgttc cttccggttg gattgaccta cgaacgcccg caccgctttg gcggcagcgt
     6061 gacggtgcag gccgggccgc ccgttgctgt ggacgatttt gccgatgcct gggctgccga
     6121 tcccgtgcag gccgtccgcg aactgaccca acatctggaa ctaaaaatgc agcagttggc
     6181 cgttcacact accgacgaag ccgaagacaa actcctgcat tacgccgaaa tttnnnnnnn
     6241 nnnnnnnnnn nnnnnnnncc gctgcctcaa aaagaagaat acctgcgcac gcgccgcctc
     6301 cttgatgggt tccgcggcct gcaacaaacc gatgcgccgg gctgggagcg cttcagccgc
     6361 gatgtacggg cttacttttt caggctgcac gcgctgggct tgcaggacca cgccgtagcg
     6421 gccccatccc ttcagttcgt gtgggggcat attgccgcat tgtggctcac tttgcctgtt
     6481 tttgtactcg gatgggcgct caacgcgctg ccttttcact tgccgggctt cattgccgac
     6541 cgcgccgggg ccgtgccgga atacaaggcc acctggaagt atgtgggcgg cctgttgttt
     6601 ttcacgatat ggtatgttat tctttatcaa ttatttagaa ctcaattttc cgttttatgg
     6661 agttggggcc tgctgttgct cacgcctgcg cttggctggg ctgcctggcg gtattaccaa
     6721 tgggctttgc atacctggca aagctggaaa gcctggaatg tattcagaaa aaaaccggag
     6781 ctgcggcaag aatggcagga gtttcgggcg gcgctgttgc aggagacgaa agggatggtg
     6841 atttaaaagg ctttacctca attcagcccc gtcacaaaca cctctatcct gccgcgcagc
     6901 cctatttgca cccggatggc ttccggcgcc agccaggcat tttgtatttc gagctggtcc
     6961 aatgagcctt tcagatagat gctctgcgat actgggtaat ggctgagttt ttggtgcagc
     7021 tccttttcca attcgttgag attctggtct aaataaaaat ccatcttttc ctctatggtg
     7081 cgttt
//
LOCUS       DAORLF010000245         7077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5741_length_7077_cov_1.927941, whole genome shotgun sequence.
ACCESSION   DAORLF010000245 DAORLF010000000
VERSION     DAORLF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5741_length_7077_cov_1.927941"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>792
                     /locus_tag="PK198_17325"
     CDS             <1..>792
                     /locus_tag="PK198_17325"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40559.1"
                     /translation="TVTLNAGVSESGAPSVSIQTTDSWCGQPTGTATVPEVGNFSYAW
                     ESASQPGAIISTNNSATGLAAGTYAVTVTNAQGCEAVRIAAVQEIPDMVVSHSATAAL
                     CFGAADGSATVVVTNGGTGPFEVQWSNHQSGNTAVGLAAGNYQYTVTDANGCTETGST
                     TVSQPAALSVNPAGAIQPTCQTSVNGSISVQVGGGTQPYDFQWDSGQQTANISGLAPG
                     DYRLTVTDANGCQASFQASLTAAGSLSVSVNTAAPSCAGLNNGSA"
     assembly_gap    793..802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <803..>1804
                     /locus_tag="PK198_17330"
     CDS             <803..>1804
                     /locus_tag="PK198_17330"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40560.1"
                     /translation="TPGVFTYQWSVPGAGSSLSNLAPGSYSVTVSNAEGCVAVQSFVV
                     PSAPAIQLQAASTPACLNDPAGTAMATATGGAGGFTYLWSNAQTGASISNLLPGVFTV
                     TATDANGCTQSEVVIVEGAPFPSLTVDQVTQPDCLGQPTGTAVVSAAGGTGMITYLWS
                     DPAGQTTAAAGGLLPGAYTVTATDENGCSAATSVTIDFPADFIPTASGIQLPLCFGQS
                     NGAAAVTVQGGSGSFSYQWNDPAAQTGAQAMNLAAGNYSVSVTDNASGCVEILNLTVA
                     QPPVLQLTASNTQNALCAGQSNGSATVSATGGTGVYTYLWNDPAAQTGPTASNLPAGT
                     "
     assembly_gap    1805..1831
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1832..>2918
                     /locus_tag="PK198_17335"
     CDS             <1832..>2918
                     /locus_tag="PK198_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF40561.1"
                     /translation="TDANGCTAQTQVELNEPQILEAQIAGFSAPLCFGQSNGSATVAV
                     TGGTGSYSYQWNDPALQNSATAANLAPGNYTVTVRDANNCPATTSVTIPNTPQIVITP
                     LDQTPPLCAGSGNGSLSATAAGGTGTLNFQWSNGQSSPSLSGLTAGNYTLSVTDGNGC
                     VQTRSFSLLDPDALVLTTANTTPATCCTGADGSISIGLQGGTGAYVYLWSDPASQTTA
                     TASGLARGDYSVTATDANGCTIVEQFSITSLGLQIFITPIVANTSCEGAANANISLGV
                     SGGAGGFSYAWSGGQSGSLLSNVGAGTYVVTATDAAGCTETAAITVENGAPFRIDIGP
                     ADTLLCEGERFFVDFSNTDYTVQWTSLS"
     assembly_gap    2919..2928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2929..3590
                     /locus_tag="PK198_17340"
     CDS             <2929..3590
                     /locus_tag="PK198_17340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40562.1"
                     /translation="FSSQAKLTALSQADTYFLRVTNAQGCAAFDTIRVAVVAEPLQAF
                     FVIPTDVVVGQEVVALEGSWPLPGQVNWYFPTDSVELLRREGDQYFFRFTQVGNVRLR
                     MQSILGGCEDWIQKVITVHADSTTIPGLNPDEPDMLGIVVAPNPNDGQFSVQVALSGP
                     KDIVLSLYNINGVVQERRIRQGQSSYTENFVVSGQPGTYFLIVQSPKQRRTYAIQVVG
                     R"
     gene            3841..>7077
                     /locus_tag="PK198_17345"
     CDS             3841..>7077
                     /locus_tag="PK198_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF40563.1"
                     /translation="MEAISIEQGLSQGFISGMAQDEDGFLWFTTSNGLNRYDGYEFKV
                     FRNDPYDSLSIGDNGLFSVAACGEFLWLMPSALQFNIFHRATQRAFPVPIDLSQLQEF
                     LKVVPEKKNAVWLLIKMNDDQKLFQLRWRQDFMAQVKKGVPLKKLFQWQEIASGVQDM
                     ALSEDEQQLWILGKDKLTVRELSTGATRPIPLPPDMKNLQWERNQLLGNVSIVPDMAG
                     AAWLYAHNQLARYDGQQWKVFPFQFAVNGLLHADRKGRLIWLRADKMVYGFQMERMPD
                     APTVQDADYSLLVPEQAICGLTDRDGNLWLGTNTWGLRKFSPRRSIFKNYFAGHSYYA
                     APIFDHRGHAWLGEPGRGTMSGGVMNLSNGEFTTFSKLGLFVQSNEALRIVKGENDRM
                     WIATGNGWNLPGGKIDPVLIEYSLKDGPINIIPFPASFYSPVRFEMIYQAPGQIWLAN
                     HYQLMRFDIATRTFSIFEMPQPPKGLSGIFAFVKTPDGAWWAGTPNGLWKAQPNEKGN
                     FTFSMLRTNPADRNSLPGNVVKSLLPDPEHPNLLWIGTSDNGMTCLDISQNRFTHYNT
                     ENGLPDNTVYGIVPEPVTGKQKSRAFWISTNRGLARFNPQNANFQYFFKSDGLQENEF
                     NTYAHGATPSGELIFGGINGFTIFNPADISVSGRLPLVRITNLRINGKTIGPRSPDDI
                     LSQDISLTQRITLQYRQNSLQLSFAADDYTNPGRNQFAFYLEGAETEWAHRGFEHSAQ
                     YLNLAPGRYVFHVKAANSDGIWNETPVSLIIVISPPWYLTGWAYGLYALLLAGAGYAF
                     YRVQLRRKLERAEADRLKEMDEFKSRFFTNISHEFRTPLTVILGVTEQLNTEVAPALQ
                     SKTNLVHRSGKNLLRLVNQILDLAKLESNTLKINYVQGDVQTYLRYITESLHSLANAQ
                     NVMLRVERKEAEIVMDYDPERLLQIVHNLLSNAIKFTPSGGRVTIHTALRNTEGTEHL
                     ELRVSDTGVGIPPEDLPHLFDRFFQARNQEFATSGGTGIGLSLTKELVKAMGGRISVQ
                     SEVGKGSTFIVELPVRKDSERLAVGSEQLAVGSRQLAVGGEQLAVGGGH"
BASE COUNT         1601 a         1958 c         1981 g         1490 t
ORIGIN      
        1 gcaccgtgac attgaatgcg ggtgtgtcgg aaagcggcgc gcccagcgtc agtattcaaa
       61 ctaccgactc ctggtgcggg cagcccacag gtacggctac agttccggag gtcggcaatt
      121 tcagctacgc ctgggaaagt gcgagccaac cgggcgccat catttctacc aataattcgg
      181 ctacaggttt ggcggcaggc acttacgcag tgactgtgac caatgcgcaa ggttgtgaag
      241 ccgtgcgtat agctgccgtg caggaaatac cggacatggt ggtaagccat tcggcgacag
      301 ccgcgctttg tttcggcgct gcagacggct cggcgacggt ggtcgtaacg aacggcggaa
      361 cagggccttt cgaggtgcaa tggagcaacc atcagagcgg caataccgca gtaggattgg
      421 ctgccggaaa ctaccaatat accgtcacgg atgccaatgg ttgcaccgaa acgggcagca
      481 caacggtgtc gcaacccgca gctttgtcgg tcaatccggc aggcgctata cagcccactt
      541 gccagacttc cgtcaacggc agcatctccg tgcaggtcgg cggcggcaca caaccttatg
      601 attttcaatg ggattccggc cagcaaacgg ccaacatcag cgggcttgcg cccggcgatt
      661 accgcctgac ggtgacggat gccaatggtt gccaggcgag ttttcaggca tcgctcacag
      721 ccgcaggttc gctcagcgtt tccgtgaata cggctgctcc cagctgcgcc ggcctcaaca
      781 acggctccgc ggnnnnnnnn nnactccggg cgttttcact taccagtgga gcgtgccggg
      841 tgcaggcagc agcctgagca acctcgcgcc gggcagctac tcggtgacgg tgagcaatgc
      901 cgagggttgt gtggccgtac aaagttttgt agtgccctct gctccggcca ttcagttgca
      961 ggcggcttcc acgccggcct gtctcaacga tccggcgggt actgcaatgg ctacggctac
     1021 gggcggcgcg ggcggattta cctacctgtg gagcaatgca caaacgggcg cttccatctc
     1081 caacctgcta cccggcgtat tcaccgtgac ggctacggat gccaacggct gcacgcagtc
     1141 ggaagtagtg attgtggaag gcgcgccctt ccccagcctg accgtagatc aggttacgca
     1201 gcccgattgt ttggggcaac ccaccggtac ggcagtcgta tcggcagcgg gcggcacggg
     1261 catgattacc tacttgtgga gcgatccggc agggcagact acggctgcgg caggcggcct
     1321 tctgcccggc gcttacaccg tgaccgccac cgatgaaaac ggctgttcgg cggccacttc
     1381 tgtgacgatt gactttcccg ccgatttcat tccgacggct tccggcattc agttgccatt
     1441 gtgtttcggg cagagcaacg gagccgctgc cgtgacggtg cagggcggtt cgggcagttt
     1501 ttcctaccaa tggaacgacc ccgcagccca aaccggcgca caagccatga atctggcggc
     1561 aggcaattat tccgtttccg taaccgacaa tgcgagcggt tgtgtggaaa tactaaatct
     1621 gactgttgcg caacctcctg ttttacaact cactgcaagc aatacgcaaa acgcgctgtg
     1681 cgccggtcaa agcaacggca gcgccaccgt gagcgccacg ggaggcaccg gggtctatac
     1741 ctacctatgg aacgaccctg ccgcacaaac cggcccgacg gcaagcaacc tcccggccgg
     1801 cactnnnnnn nnnnnnnnnn nnnnnnnnnn ncaccgatgc caacggctgc actgcacaaa
     1861 cgcaggtaga actcaatgag ccacagatac ttgaggcgca gattgccggt ttcagcgcgc
     1921 cgctttgttt tggtcagagc aacggctcgg ctacggtagc ggtcaccggc ggaacgggca
     1981 gttattccta ccaatggaac gaccctgcgt tgcaaaattc cgcgacagcg gccaacctcg
     2041 cgcccggcaa ctacacggtg acggtgagag acgccaacaa ctgtccggca acgacttccg
     2101 taacgattcc caacacgccg cagatcgtca ttacgccgct tgaccagacc cctccgctct
     2161 gcgcgggatc gggcaacggc agcctcagcg cgacggctgc gggcgggacc ggtactttga
     2221 atttccaatg gagcaacggg cagagcagcc cttccttgtc tggtctgact gcaggcaatt
     2281 acaccctgag cgtgacggac ggcaacggtt gtgtgcaaac cagatcgttc agtctgcttg
     2341 atcccgacgc gctggttctg accacggcca acaccacgcc ggccacttgc tgcaccggcg
     2401 ccgacggcag catttccatt ggtttgcaag gcggtacagg cgcatacgtt tacctgtgga
     2461 gcgatcccgc cagccaaaca acggccacag cttccgggct ggctcgcggc gattattccg
     2521 taacggccac cgatgccaac gggtgtacta tcgttgagca attcagcatc acttcgctgg
     2581 ggctgcaaat tttcatcacg ccgatagttg ccaatacttc ctgcgagggg gcggccaatg
     2641 ccaatatttc gctgggggta tcgggcggcg cgggcggttt cagctatgcc tggagcggcg
     2701 gccaaagcgg cagcctgctt tcaaatgtgg gtgcgggtac ttatgtggtc acggctaccg
     2761 atgcggccgg ttgtaccgaa actgctgcca tcacggtgga aaacggagcg cctttccgca
     2821 tagacatcgg cccggcagat acgctgctgt gcgaaggcga gcgctttttt gttgatttca
     2881 gcaacaccga ctacaccgtg caatggacca gcctgagcnn nnnnnnnngt ttttccagtc
     2941 aggcaaaact gacggcactg agccaggccg atacttactt cctgcgggtg accaatgcgc
     3001 agggttgtgc cgccttcgat accatacgcg tggcggtggt agccgagcca ttgcaggctt
     3061 tctttgttat tcctactgat gtggtggtgg ggcaggaagt ggtggctttg gagggttcgt
     3121 ggccgctgcc tggtcaggtg aactggtact ttccgacgga cagcgtagaa ctgctgcgcc
     3181 gcgagggcga ccagtacttt ttccggttca cgcaagtggg caatgtgagg ctgcggatgc
     3241 agagcatttt gggcggctgc gaagactgga tacaaaaagt catcaccgta cacgccgaca
     3301 gcacaacgat accgggtctg aatcctgacg agcctgatat gctcggcata gtggtagcgc
     3361 ccaacccgaa cgacgggcag ttttcggtgc aggtcgcgtt gtcggggccg aaggatattg
     3421 tgttgagttt gtacaacatc aacggggtgg tgcaggagcg ccgcatccgg caggggcaga
     3481 gcagctacac ggaaaatttt gtggtgagcg gccagccagg aacgtacttc ctcattgtac
     3541 aaagtccgaa acagcggagg acttatgcca tccaggtggt ggggcggtaa tctgcctgcg
     3601 gcggtaatct gcctgcggca gttcatctgc cttcggcagt aatctgctgc gcagttgatc
     3661 tgttaacgga gaggggagtg ttttatcggc ttttttgtac aatccgatct gtaaaaaaac
     3721 aggcgcgcaa tacagcgcaa atgggcgtat ttttgcatcc tgtaaccaac acagatgctg
     3781 cgaaccgcct ttttcctgct ccttttgtgc cttgcgagat tgcaagccca aacgcccgcc
     3841 atggaagcca tatccatcga gcagggcttg tcgcagggtt ttatctccgg catggcccag
     3901 gatgaggacg gctttttgtg gttcaccacc agcaacggac tgaatcgcta tgacggctac
     3961 gagttcaagg ttttcagaaa tgatccttac gattcattgt ctatcggcga caacggcctg
     4021 ttcagcgtgg cggcctgcgg cgagtttctt tggctgatgc cctccgcatt gcaattcaac
     4081 atttttcacc gggctacaca acgggcattt ccagttccaa tagacctgtc tcaactacag
     4141 gaatttttga aagttgtacc cgaaaagaaa aatgcagtgt ggctgctgat aaaaatgaac
     4201 gacgaccaga agttgtttca actccgatgg cggcaggatt ttatggctca ggtgaaaaaa
     4261 ggcgtgcccc tgaaaaagct gtttcagtgg caggaaatag cctccggggt gcaagacatg
     4321 gcactctcgg aagatgagca acagttatgg atattaggca aagacaagct caccgtacgc
     4381 gagctttcta ccggcgctac ccggccaata cctctgcctc cagatatgaa aaacctacaa
     4441 tgggaaagaa atcaactgct tggaaatgtg agcattgtac ccgatatggc aggcgctgca
     4501 tggttatacg cccacaacca attagcgcga tatgacggtc aacaatggaa ggtttttcct
     4561 tttcaatttg ctgtgaacgg cctgttgcat gccgaccgaa agggccgcct gatctggctc
     4621 cgggccgata agatggtata tggatttcag atggagcgta tgccggatgc gccaaccgtt
     4681 caagatgccg actacagcct gttggtaccg gagcaggcca tttgcggcct taccgaccgc
     4741 gacggcaacc tctggctggg taccaatacc tggggcctcc ggaagttttc gccccggcgc
     4801 agcattttca aaaattactt tgccggccat tcttattatg cagcccccat tttcgatcat
     4861 aggggtcatg cgtggctggg cgaacccggc cggggcacca tgagcggcgg cgtaatgaac
     4921 ctttccaacg gagaattcac cactttttcc aaactggggc tgttcgtaca aagcaacgaa
     4981 gccctgcgca ttgtaaaggg agaaaacgac cgaatgtgga ttgctacggg gaatggatgg
     5041 aatttgccag gtggtaaaat tgatccggtt ttaattgaat attcgctcaa agacggtccg
     5101 ataaacatca ttccctttcc tgcatcattc tacagtccgg tacgctttga aatgatctac
     5161 caggcccccg gtcaaatctg gttggccaat cattaccaat tgatgcgctt tgacatcgcc
     5221 acccgcactt tttctatatt tgaaatgccg cagcctccga aaggactcag cggaattttt
     5281 gcctttgtaa aaacgcccga cggcgcatgg tgggccggaa ccccgaacgg attgtggaag
     5341 gctcaaccaa acgaaaaggg caattttact ttctccatgc tgcgcaccaa ccccgcagac
     5401 cgcaacagtc tgcccggcaa tgtcgtcaag tctttattgc ccgaccccga gcatcccaac
     5461 ctgctctgga taggtaccag cgataacgga atgacttgtt tggacatctc ccaaaaccga
     5521 ttcacgcact acaataccga aaacggattg cccgacaata cggtctatgg cattgtacct
     5581 gagcctgtta ccggcaaaca gaaaagtcgc gccttctgga tcagcaccaa ccgagggctt
     5641 gccagattca accctcaaaa tgctaatttt caatattttt tcaaatcgga cgggcttcag
     5701 gaaaacgagt tcaataccta tgcgcacgga gccactccat ccggcgaact cattttcggc
     5761 ggcatcaacg gttttaccat tttcaacccc gcagatatca gcgtaagcgg ccggttgcct
     5821 ttggtgcgca ttaccaatct gaggataaac ggcaaaacaa tcggcccccg aagccccgac
     5881 gacattcttt cacaagacat tagtttaacc cagcgcatca cacttcagta ccgtcaaaac
     5941 agcctacagc tatccttcgc cgcagacgac tacaccaatc ccggccgcaa tcaattcgca
     6001 ttttatctgg agggcgccga gaccgaatgg gcgcatcgcg gcttcgagca cagcgcccaa
     6061 tacctcaatc ttgcccccgg ccggtatgtt tttcatgtaa aagcagccaa cagcgacggt
     6121 atctggaacg aaacccccgt atcgttgatc atcgtgataa gtccgccctg gtatctcaca
     6181 ggctgggctt atgggctgta tgcgctcttg ttggcgggtg cgggatatgc cttttaccgg
     6241 gttcagttga ggcgaaaact cgagcgagcg gaggcagacc ggctgaaaga aatggacgag
     6301 ttcaagtctc gcttttttac caatatttcc cacgaattcc gaacgcctct gacggtcatt
     6361 ttgggcgtta cagaacaact gaacaccgaa gttgccccgg ccctgcaaag caaaaccaat
     6421 ctggtgcatc gcagtggaaa aaacctgctg cgattggtaa accagatatt agacttagcc
     6481 aaattggaga gtaacactct gaaaataaac tatgtacaag gtgatgtgca gacctacctg
     6541 cgctacataa ccgagagcct gcactcattg gccaacgccc aaaatgtgat gctgcgggtg
     6601 gagcgcaaag aggcggaaat cgtgatggac tacgaccccg aacgcctgct tcagatcgtt
     6661 cacaacctgc tttccaatgc catcaaattt acgccttccg gcggacgcgt caccattcat
     6721 acagctttgc gcaacacgga aggcacagaa catctggagc ttcgggtgag cgacacaggg
     6781 gtgggcatac ccccggaaga cctgccgcat ctgttcgacc gcttttttca ggcgcgcaat
     6841 caggagtttg caacatccgg cggcacgggc atagggcttt cgctgaccaa agaattggtg
     6901 aaagcgatgg gggggcgcat ttcggtgcaa agcgaagtgg gaaaaggaag tacgttcatc
     6961 gttgagctac cggtgaggaa ggatagtgag cggttggcgg ttggcagtga gcagttggcg
     7021 gtaggcagta ggcagttggc ggttggcggt gagcagttgg cggtaggcgg tgggcac
//
LOCUS       DAORLF010000246         7068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5751_length_7068_cov_1.788250, whole genome shotgun sequence.
ACCESSION   DAORLF010000246 DAORLF010000000
VERSION     DAORLF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5751_length_7068_cov_1.788250"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>326
                     /locus_tag="PK198_17350"
     CDS             140..>326
                     /locus_tag="PK198_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRF40564.1"
                     /translation="MNNSELMQRIDLALDDVRPHLAVDGGNVELVEITEDNIVRIKWT
                     GACEHCSMSIMTLRAGIE"
     assembly_gap    327..383
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    706..771
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <772..>1057
                     /locus_tag="PK198_17355"
     CDS             <772..>1057
                     /locus_tag="PK198_17355"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF40565.1"
                     /translation="IVVNGQNIEDAGVRYRATRSFQPGGSRNGLDILLNHVDANTNYQ
                     GYSSIKLSSALRDPSMVREVLGYEIARQYMPAPKANYARVTINGKLYGLFV"
     assembly_gap    1058..1153
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1154..2218
                     /locus_tag="PK198_17360"
     CDS             <1154..2218
                     /locus_tag="PK198_17360"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF40566.1"
                     /translation="DAKAQEGCRSNIFGSLQVDNSADCYRQNYELVMGFGYDPILGLA
                     KMLNEKPDDIASVLHVDRTLWMLAYNNALLNLNSYTGQYSSNYFLYRGKAGQFTPILW
                     DLNLNFGSYKNTGVGSDLKLASVIQLDPLLHSNNSERPLISKLLSNELYKKMYLSHLV
                     TINNDYLANEKYLNRAKELQQLIRPDLAKDVNWASFYTMAEFDQSLKMTIGKKSKIPG
                     LTEVLTPRAEFLKKHPELMVLPPSISDITIKHREKLSAVMVGTFKIQAKVGEFPKRVK
                     IFYRFSSDEPFREAMMNDDGKSADETANDGIYGIDIPPFRNARSIEFYIMAENARMVT
                     YSPARYMYELHKTNLDELNQ"
     gene            2202..>2413
                     /locus_tag="PK198_17365"
     CDS             2202..>2413
                     /locus_tag="PK198_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40567.1"
                     /translation="MNSTSNEISNPSTGFLLRLIGFRALHSAARWGCFFVLCLWALAG
                     NAQAQDFYKPGAVVEIRLVFKEKNWD"
     assembly_gap    2414..2548
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2549..>3709
                     /locus_tag="PK198_17370"
     CDS             <2549..>3709
                     /locus_tag="PK198_17370"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF40568.1"
                     /translation="KSGSAKLPFNIKIDFTEKNQSLPGGITTLKLANSFRDPSFLREV
                     LAYEIAGQYMPAPKANFAKVFVNEEFIGLYSNTESVDKPFLQRHYDKGKGALLKCDPA
                     DWNAKMPAGCSPSDQASLQYLGNDSLCYQGIYEVEQGYWADLIQFTDILNRKPELLDS
                     VLNIDAALWMLAFDNVTVNLDSYLGKFCHNYYLYKDSSGVFHPILWDLNMAFGGFRYN
                     GVEEAAMSDEKLQTLSPFLHYREKNMKRPLILQLLSNDLWRKVYIAHIRTLLEEHFAN
                     GAYKKRAQELQKMLDPTVQEDKNKLYDYEGFKSNIDTIAKAEKSNIIGIAQLMDRRAA
                     YLAEHPLLQKTPPVISDAKHTLQKKQTTIVTCKTENARNVWVLYRQGKTGVF"
     assembly_gap    3710..3801
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4091..4188
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4189..4882
                     /locus_tag="PK198_17375"
     CDS             <4189..4882
                     /locus_tag="PK198_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF40569.1"
                     /translation="LAWNCRSCSGEMNRLPRLYHHGEIGDFGQVIQHAFRTKDYEQVA
                     LVGFSMGGSITLKYLGVQGKELPDAVKTGIAFSTPCDIEASIRTLELPVNRFYKNRFY
                     KKLRRKIELKAIQFPGLIDAGLLNQVKTWRDFDTFFSAPLNGFDSPETFYQQASCVNY
                     MEGIRVPALLANAQNDPILSPECSPAELCRRHPNVYLEMPARGGHVGFTLHRRGFSWM
                     EQRAMEFIQNGK"
     gene            5314..6342
                     /locus_tag="PK198_17380"
     CDS             5314..6342
                     /locus_tag="PK198_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF40570.1"
                     /translation="MKKLMQLSLVILAVLSIAFTGCKKDDIVTPETSYETLSKYVAQN
                     NLDLPTIVSGFTKSGSQININMTDFSVPDYFVIDVRAAADYNTGHIKNAVNVPIANLL
                     EAVASVPKDQNILVTCYTGQTASRAVVALRMMGYTKVYSLKWGMASWHADFASRWDSK
                     VANVTSPGWVNTGAPTVSTTGALPTFTSTATTGDKILEERVKATLANTSWTVNRDDVL
                     ANPGNYFINNYWPAVSWDAFGHFAGAYRIFEDLKVENLGLLRTDKPLVTYCYTGQTSG
                     IVTTWLQIMGYDNARSLSFGANAIVYDQLKVSAVDDAKKKSWKGEGSGSANNFGYYDS
                     TNTMHNPL"
     gene            6522..>7068
                     /locus_tag="PK198_17385"
     CDS             6522..>7068
                     /locus_tag="PK198_17385"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF40571.1"
                     /translation="MKNLIVLTISAFLLMPSFMNAQACMSPTSDEGISIIGFIQPQYE
                     YLFAGQDQQGKRLDESSFYFNRARLGVTGNIPYDFSYYFMTEISPTLNGAKNIQPPLL
                     LDAFISYNRFAPYVKVSAGQFKVPFGIELPQPCHKLYTINRSMVVANLVDPWRDLGVM
                     VSGGTGDLSILGTKTKNAVSYS"
BASE COUNT         1700 a         1722 c         1526 g         1576 t
ORIGIN      
        1 agtagccaaa aatacagccc ggcaagtggc gctgcgcaac gaaacactgg cgcccaccca
       61 agtggtgaat gtgcaaggct gatgctgatg aatttatatt acctttgtgt ccataccact
      121 tgatacgatg acaacaacaa tgaataattc cgaacttatg cagcgcattg acctcgcatt
      181 ggacgatgtg cgcccacacc tcgcagtgga cggcggcaat gtggaattag tggaaatcac
      241 agaggacaat attgtcagaa tcaaatggac aggagcctgc gagcattgca gcatgtctat
      301 tatgacattg agagccggca tcgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnngctccgg tctgattaga ttcagctatt gcgacgtttc
      421 ttgaacaagc ccccaaacgc ctacttgccg gagcaatccg agccgttgtg caaactaccg
      481 ttaaattatt tacccgcccc tccgaagccg ttacttgcca agtttttctt aatattgcgt
      541 cgtcaaacca aaagacaatt gcaatccaat gaaaaaccgg tttatacaac atctccctct
      601 cttttgggtg ctcctgtcgg gcatactctc aacccaggct tacgctcaaa aagactttta
      661 cgacgtttcc accattcaaa acatcgagat taagtttgag caggannnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncattgttgt
      781 caatggtcag aatatagaag atgccggcgt ccgttaccgg gctacgcgct ccttccagcc
      841 aggcggtagt cgcaatggat tggacattct gctcaatcat gtggatgcca atacgaacta
      901 tcagggctac tcgtccatca agctgtcgag cgccctccgc gatccgagca tggtgcgcga
      961 ggtattgggc tatgaaatcg cccggcagta catgcccgcc ccgaaggcca actatgccag
     1021 agtaacgatc aacggcaaat tgtatgggct tttcgtcnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnngatgcga aggcgcagga aggctgccgc agcaatattt tcgggtctct
     1201 tcaggtggac aactccgcag attgctaccg tcagaattac gagttggtca tgggcttcgg
     1261 ctacgaccct atattgggct tggccaaaat gctcaatgaa aagccggacg atattgccag
     1321 cgtactccat gtggaccgta ccctgtggat gttggcctat aacaacgcgc tgctcaacct
     1381 gaacagttac accggccaat acagctccaa ctatttcctt tacagaggaa aagcagggca
     1441 attcaccccc attctgtggg atttgaacct caatttcggc agttataaaa atactggtgt
     1501 aggctctgat ttgaaattag catcggtcat tcagctcgac ccgctgctgc atagcaacaa
     1561 cagcgaaagg ccgctcatca gcaaattgtt gagcaatgag ttgtacaaaa aaatgtacct
     1621 gtctcatttg gtaaccatca acaatgatta cctggccaac gaaaaatatc tcaaccgcgc
     1681 caaagaactg caacaactca tccgaccgga tttagccaag gatgtcaact gggcttcatt
     1741 ttacaccatg gccgaatttg accaaagctt gaaaatgacc attggtaaaa agagcaaaat
     1801 tccgggtttg actgaagtgc tgacgcctcg tgccgagttt ttgaaaaaac atcccgaact
     1861 catggtgttg ccgccctcca tcagcgatat caccatcaaa caccgcgaaa aactgtcggc
     1921 ggttatggtc ggtacgttca aaatacaagc taaagtgggc gagtttccca agcgagtcaa
     1981 aatcttctac cgttttagtt ctgacgagcc gttccgtgaa gccatgatga acgacgacgg
     2041 aaaaagcgcc gatgaaactg ccaatgacgg gatttacggt atagacattc cgcctttcag
     2101 aaatgcacgc agcatcgagt tttacatcat ggccgaaaat gcccgcatgg tgacatacag
     2161 cccggcgaga tacatgtacg agcttcacaa aaccaatctg gatgaactca accagtaacg
     2221 aaatttcaaa tccgtcaacc gggtttttgc tccgcctcat cggttttcgc gcacttcata
     2281 gcgccgcccg atggggctgt ttttttgtcc tctgtctgtg ggcattggcc ggaaacgcac
     2341 aagcgcagga tttttacaaa cccggcgccg tagttgaaat tcgcttagtt tttaaagaaa
     2401 aaaactggga cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aaaagtggct ctgccaagct ccctttcaat
     2581 atcaaaatag attttaccga gaaaaatcag tcgctgccgg gaggaatcac cacgctcaaa
     2641 ttggccaact cttttcgcga tccgagcttt ctgagggagg tattggctta cgaaattgcg
     2701 ggccagtata tgccggcgcc gaaggcgaat tttgctaaag tgtttgttaa tgaagagttt
     2761 atagggctgt atagcaatac agaatcggtg gacaaaccct ttctgcaacg ccattatgac
     2821 aaaggcaaag gcgcactcct caaatgcgat ccggcagact ggaacgccaa aatgccggcg
     2881 ggctgctctc ccagcgacca ggcatcgcta caatatctgg gcaacgattc gctctgctat
     2941 cagggcattt atgaggtaga gcaaggttat tgggccgacc tcattcagtt taccgatatt
     3001 ctcaaccgca agcccgaact gttagactcc gttttgaaca tagacgccgc cctctggatg
     3061 ttggcattcg ataatgtgac ggtcaatttg gacagctacc tcggcaaatt ttgccataat
     3121 tactacctct acaaagacag ttccggtgta tttcacccca tcctgtggga tttgaacatg
     3181 gctttcggcg gcttccggta caatggagtg gaagaggcgg ccatgtcgga tgaaaaattg
     3241 caaacgctca gtccgttttt gcactatcgg gaaaaaaata tgaagcgtcc actcatcctg
     3301 caattgctct ccaacgacct ctggcgcaag gtatatatcg cgcacattcg cacgctgctg
     3361 gaggagcact ttgccaacgg agcctacaaa aaaagagcgc aggaattgca aaaaatgctc
     3421 gatccgacgg tgcaggaaga taagaacaaa ctctatgatt atgagggttt taaaagtaat
     3481 atagacacca ttgccaaagc cgaaaaatca aatataatcg gtatagcaca attgatggat
     3541 cgccgggcag cctatttggc tgagcacccg ctgctgcaaa aaacgccccc ggttatctcc
     3601 gacgcaaagc ataccttgca aaaaaaacaa accaccatcg tgacctgcaa aacagaaaat
     3661 gcccgcaacg tgtgggtgct ttaccggcag ggcaaaacgg gggtgttccn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn ntacggcaaa gggctgacgt attatttcat tgcagaagac
     3841 gagcgcaacg cgacggtgtt gccgcgcaag gcttcgcacg cgccttttga ggtacagtaa
     3901 atcctgcttt gtatgccgct gattgcatct tcttcttacc tccaatcgcc ggcatacctg
     3961 tggaacggcc atgtggaaac cattgtgccc gcagtagccc ggcgcgtgaa agtgccttat
     4021 gagcgggagc ggctcgaatt gcccgacggc gatttcgtgg atttggactg gttggacaaa
     4081 aaatcccgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tggcctggaa
     4201 ctgccgctcg tgcagcggcg aaatgaaccg gctgccgagg ctttaccacc acggcgagat
     4261 cggcgatttc gggcaggtca tccaacatgc gtttcgcacc aaagactatg agcaagtggc
     4321 gttggtagga ttcagtatgg gcggcagtat taccctcaaa tatctgggcg tgcaaggcaa
     4381 agaactgccc gacgcggtca aaaccggcat cgcgttttct accccctgtg acattgaggc
     4441 cagcattcga accctggaac tgccagtcaa tcgcttttat aaaaaccggt tttacaagaa
     4501 gttgcgcaga aaaatagaac tcaaggccat tcagtttccc ggactgatag acgccggctt
     4561 gctgaatcag gtcaaaacct ggcgcgattt cgataccttc ttctccgccc cgctcaacgg
     4621 attcgacagc cctgaaacct tttatcagca ggcctcctgc gtgaactaca tggaaggcat
     4681 tcgtgtgccg gccttgctgg ccaacgccca aaacgacccc attttatcgc ccgaatgttc
     4741 tcccgcagag ctgtgtcgcc gccatccgaa tgtatatttg gaaatgcctg caaggggcgg
     4801 ccatgtaggc ttcacgctgc accgtcgggg ctttagctgg atggaacagc gggcaatgga
     4861 gtttattcag aatggaaagt aagcccttta tccggttatt ctcattttgt tttgcttgaa
     4921 atttgcggaa cttcgttttg tttatgttcg tgttttgtaa aattctggac taatactact
     4981 gggttattta tgtggtgttt gaaatgttat tcgttttgaa ttgtctttgg gggttaattt
     5041 ttaatgatag ttttgtgtgt tgacaattat caccgccctg gatgaccgaa atcagttttt
     5101 ttccatttgt ggcaccccta tttttgcact gtgataaatg aaaaaagttt cattcatcag
     5161 atttgataag gattgaatta aaaatcattc gcggccgggc tgcggagcaa aaattcaggc
     5221 ctgaaatttt tcgatacgcg ttagaagaat tcacacctcc atttttcctg ccggcagcag
     5281 tcgcatccat ttcataacat taaactgctt gccatgaaaa agttgatgca actttcatta
     5341 gtcattctcg ccgttctttc gatcgcgttt actggttgca aaaaggacga catcgttact
     5401 cccgaaactt cgtatgaaac cctcagcaaa tacgtagcac aaaacaacct cgacttgccg
     5461 accatcgtat ccggcttcac caaatcgggc agccagatca acatcaacat gaccgacttt
     5521 tccgttcccg attatttcgt gattgacgtt cgcgccgctg ccgactacaa caccggccac
     5581 atcaaaaatg ccgtcaatgt accgatagcc aacctgctgg aagcagtagc ttctgtgccg
     5641 aaagaccaaa atatcctcgt gacctgctac accggccaga ctgcttcccg cgcagtggtg
     5701 gcgctccgca tgatgggcta caccaaagta tattcactca agtggggcat ggcaagttgg
     5761 cacgccgatt tcgcttcaag gtgggattcc aaagtggcca acgtcacttc tccgggttgg
     5821 gtgaacaccg gcgcgcccac cgtttctacc accggcgctc tgcctacctt cacttccacg
     5881 gctaccaccg gcgataaaat tttggaagaa agggtaaaag ctactttggc aaacacctct
     5941 tggacggtca accgcgacga tgtgctggcc aatccgggca actattttat caacaactac
     6001 tggcctgccg tttcctggga tgctttcggt cacttcgccg gcgcataccg catctttgaa
     6061 gacctcaaag ttgaaaatct gggtctgctg cgcaccgaca agccattggt tacctactgc
     6121 tacaccggcc aaacttccgg tatcgttact acctggctgc aaatcatggg ctatgacaat
     6181 gcccgcagct tgtccttcgg cgccaatgcc atcgtgtatg accaacttaa agtcagcgct
     6241 gtggacgatg ccaaaaagaa atcctggaaa ggtgaaggct ccggtagtgc caacaacttt
     6301 ggctactacg actctaccaa taccatgcac aatccgttgt aaatcagttt gtacctgacg
     6361 tcgggggtgg gtaaccagtt ggggtaaccc ttgtggttcc cccccccgac gagcagggca
     6421 accacgaggg ttgccccgac cagcccccgg ttatcgtttt tagggcaacc acgcctcacc
     6481 ttagggcaac cacgagggtt gccccaacaa aaatcttcgc catgaaaaat ctcatcgttc
     6541 tcaccatatc ggcattttta ctcatgccgt cctttatgaa tgcacaagcc tgtatgagtc
     6601 ccacttccga tgaaggcatc tctatcatag gcttcattca accacaatac gaataccttt
     6661 tcgccgggca agaccagcaa ggcaagcgcc tggatgaaag ctcgttctac ttcaaccgcg
     6721 cccgcttggg ggtcaccggc aacattcctt acgatttcag ctactatttt atgactgaaa
     6781 tcagccctac gctcaacggc gccaaaaaca tccagccacc gctgctgctc gatgctttta
     6841 tcagctacaa ccgttttgca ccgtatgtaa aagtctccgc cgggcagttc aaagtgcctt
     6901 tcggcattga gttacctcag ccctgccaca aactttacac catcaaccga tctatggtgg
     6961 tagccaacct cgttgacccc tggcgcgacc tcggcgtgat ggtcagcggc ggcaccggcg
     7021 atctgagcat tctgggtacc aaaaccaaaa atgccgtttc ttacagcc
//
LOCUS       DAORLF010000247         7051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5771_length_7051_cov_2.346341, whole genome shotgun sequence.
ACCESSION   DAORLF010000247 DAORLF010000000
VERSION     DAORLF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_7051_cov_2.346341"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK198_17390"
     CDS             complement(<1..978)
                     /locus_tag="PK198_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRF40572.1"
                     /translation="MTYDNFTIKAQESILKAQQIAGGFNQQQVDTIHLVKGMLEADEN
                     VPNFLMQKQGVNIPQLKRQLDEAIASYPRVEGSDKQYLTNDANAALAAAKKMLKDFGD
                     EYISIELMLLGILKGNDRGAKLLKSLGMTEDGTRAAIQELRKGRKVQDPGAEDTYNAL
                     NKYGVNLNEQAESGKLDPIVGRDEEIRRVLHILSRRKKNNPLLIGEAGVGKTAIVEGI
                     AWRIVKGDVPDNLREKKIYVLDIAGLIAGAKYKGEFEERLKAVIQEVIESAGKVILFV
                     DEIHTLIGAGGGQGAMDAANILKPALARGELRTIGATTLDEYQKYFENDK"
     gene            1303..2289
                     /locus_tag="PK198_17395"
     CDS             1303..2289
                     /locus_tag="PK198_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRF40573.1"
                     /translation="MYVKRNYGFWMTFNWSKKPFILGFLYAAVITAVYHFAHINVALP
                     WQPVSVIGIAVAFYLGFKNNSSYDRTWEARKIWGGIVNSSRSFGAALMAFVQGPQAEE
                     HRKELIYRHIAWLIALRHQLRLSREWEHAEERIKGLYLPEVCEEYFNRLDDEIIHFIS
                     EQEFQFYKSKTNIATQILAKQSERLQELRDAGSFEDFRHMELQALITSFYEQQGGSER
                     IKNFPFPRQYASTALWLSMVFSVLTPFGMLDTFAQLGDWWVWLTVPMSALIIWIFFLM
                     EKIGDYSENPFEGSYNDVPISSIARAIEIDLREMIGDTDIPPAMKAQSGFLM"
     gene            2475..5222
                     /locus_tag="PK198_17400"
     CDS             2475..5222
                     /locus_tag="PK198_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF40574.1"
                     /translation="MSVLFLWLCPACKETTPEKKYRVGFAQCCDDPWRDVMNSEMYRE
                     LELHPELEFEMRVSGNESEKQVEQIREIVASGIDILIVSPNESKPLTHIIEEVYTSGT
                     PVILIDRETDSELYTAYIGADNYEIGKTAGRYLANRLNGRGKIIEIQLPLAISPAIER
                     SRGFKDGIAAAPGLEIAAVLETVGGLNDIEARFPDMLKRHSDVSAIFGHTDLLAETAY
                     KIAKEQGRGTRMFFVGIDGIPGTGRGIQAVEDGILDASLLYPTGGAEAIRLAVSILNR
                     LPYEKQNNLETIVINKDNARILHNQMKKVQSLQESIDEQMSNLAGLKSIYRNQRVYIF
                     ILLSSLLLSLVLGVFLWRSLRAKQTALDSLERKNQEILEHERQIMAMSDEVKQATQAK
                     VDFFTNISHEFRTPLTLILGFAEDLLPSPTISKDVQQSLAHIRRNAFRLLHLVNQLMD
                     FRKSESGQMKLRAAENDLIAFVKKIMESFGNTAQKRGIDFQLLSRLEQLPVWIDAGMM
                     DKVLFNLLSNAFKFTLDGGKIYLTVAVDVFENRAKITVEDSGQGIQEEDLRHIFEPFY
                     QGEERPQAGTGLGLALAKALVELHGGAIDVQSTKGRGSRFLVSLPLGNKHLRADQMTS
                     ENTAYLSHHDFLQAPDTDWQEISGEMENESAGRQILIIEDNEDLQLFLRKKLSASYQV
                     LQATDGNEGLQKALDHTPDLIICDVMLPGMNGIEITRALKSDLRSSHIPVIMLSARSA
                     TAQQIEGAEAGADIYLTKPFNIHFLQASIRSLLLNRQMLREHFSKGISAFSTADAPAL
                     PAERSANALDVDFVRQFADYMERHYARQDFQVSDLCREFGLSRSQIYRKVTALLGESI
                     SDYLQNIRLKKAEVLLAEGHLPVAEIAYQVGYSSPDYFSTVFRSRYGVPPSSFRREAN
                     T"
     gene            5450..>6651
                     /locus_tag="PK198_17405"
     CDS             5450..>6651
                     /locus_tag="PK198_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRF40575.1"
                     /translation="MNHRKIFTWSITVALAGFLFGFDTVVISGADLSLQKLWPRGEVF
                     HGFVVMASALWGTVVGALLGGIPTDKLGRKKTLFWIGVFYFVSAVGSALAADPFTFAF
                     FRFLGGLGVGASTIAAPAYISEIAPANDRGKLVALYQFNIVFGILVAFLSNYLLSGLG
                     ENAWRWMVGVEAVPALVYTVMVLGVPRSPRWLLVKKHDRDAAAKTLRLIDPGMNVEAE
                     MDRILSDQVDVVKGETIFTPKYRFALTLAFLVAFFNQFSGINAFLYYAPRIFEIAGLE
                     KSSALLSSVGIGVINLIFTLIGLSLIDRFGRRQLMYIGSVGYVISLSLVALAFMLNIK
                     GMAVPVFLFLFIASHAVGQGAVIWVFISEIFPNHLRAAGQAFGSSVHWILAAVIPSLV
                     PVLFKSIGA"
     assembly_gap    6652..6718
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1620 a         1743 c         1860 g         1761 t
ORIGIN      
        1 tttatcgttt tcaaaatact tttggtactc gtccaaagta gtagcgccga tggtgcggag
       61 ttcgccgcga gccaatgccg gtttcaggat gttggcggca tccatagcgc cctgacctcc
      121 gccggccccg atgagcgtgt gaatctcatc cacaaaaagg atgactttgc ccgccgattc
      181 gatcacttcc tgaataacgg ccttgaggcg ctcttcaaac tcgcccttgt acttggcgcc
      241 ggcaatgagg cccgcgatgt cgagcacata aattttcttt tcacggaggt tgtcgggcac
      301 gtcgcctttg acgatgcgcc atgcgatgcc ctccacgatg gcggttttgc ccacgccggc
      361 ctcgccgatg aggagcgggt tgttcttctt gcggcgggag aggatgtgca aaacgcggcg
      421 gatttcctca tcgcggccca caatggggtc gagcttgccg ctttcggcct gttcgttgag
      481 gttcacgccg tatttgttca gcgcgttgta ggtgtcttcg gcgccggggt cctgcacttt
      541 gcggcctttg cggagttcct gaatggcggc gcgggtgccg tcttcggtca tgccgaggct
      601 tttgagcagt ttggcgcccc ggtcgttgcc tttgagaatg cccagcaaca tgagttcaat
      661 agagatgtac tcgtcgccga agtctttgag catctttttg gcggcagcca gagcggcatt
      721 ggcgtcgttg gtaaggtatt gtttgtcgga gccttccacg cgagggtagg aagcgatggc
      781 ttcgtccaac tggcgtttga gctgcgggat gttcacgccc tgtttttgca tcagaaaatt
      841 gggcacgttt tcatcggctt ccagcatgcc ttttaccaag tggatggtgt ccacctgttg
      901 ctgattgaag ccgcctgcaa tttgttgggc cttgaggatg gactcctggg cttttatggt
      961 gaaattgtcg tacgtcatga tgcggttggt tgtgaagatg taatttttga aatattgcgg
     1021 agggctgcaa gccataagtc tcaagctgca agcttggctt tccaaactac aaagacgcgg
     1081 ccaaatacca ggtacagcgg cttttcttcg acaaaaaaac gaattttccc ggttttcgga
     1141 ggcatgtaag aataatggtg aggctgtaag gcaaaaaatg acaggcaggc gaggcttttt
     1201 tttgtcaaag tggcagcaat gaggcatctt ggcccgtccg cttcgcgaag atatgttttt
     1261 aaccgatttt tctacttttg gcactcaaaa tcctactcta tcatgtacgt caaacgcaat
     1321 tacggcttct ggatgacctt caactggtcg aagaagccct tcatactcgg ttttttgtac
     1381 gctgccgtta tcactgcggt gtatcatttt gcacacatca acgtggccct gccctggcag
     1441 cccgtcagcg tcatcggcat agcggtggcc ttttacctgg gtttcaaaaa caacagttcc
     1501 tacgaccgca cctgggaggc ccgcaaaatc tggggcggca tcgtcaacag cagccgctcg
     1561 tttggggccg cgctcatggc tttcgtgcag gggccgcagg cagaggagca tcgcaaggaa
     1621 ctcatttaca ggcatatcgc ctggctcata gccctgcggc accagcttcg cctcagtcgg
     1681 gagtgggaac acgccgagga acgaatcaag ggactgtatt tgccggaagt gtgcgaggaa
     1741 tatttcaacc gcttggacga tgagatcatc cattttatct ctgaacagga atttcaattt
     1801 tacaaaagca agaccaacat cgccacccag atactcgcca aacagtctga acgattgcag
     1861 gaactgagag atgcgggcag ttttgaagac tttcgccaca tggagttgca ggcacttatc
     1921 acgtcttttt atgaacaaca ggggggcagt gaacgcatca aaaacttccc ctttccgcgc
     1981 cagtatgctt ctactgcctt gtggctgagc atggttttca gcgtgctcac tcctttcggc
     2041 atgttggaca cgtttgctca actgggcgac tggtgggtgt ggctcacggt gcccatgtcg
     2101 gcgctcatca tctggatttt cttcctgatg gaaaaaatcg gcgactactc cgaaaaccca
     2161 tttgaaggct cctacaacga cgtacctatc tcttccatcg cccgcgccat cgaaattgac
     2221 ctccgcgaaa tgatcggcga tacggatatt ccgccggcga tgaaggcaca gagtgggttt
     2281 ttgatgtagg gagccgccag tatcagccgg tgaataccaa ttacggatgt tctttcctcc
     2341 cttacgtctt taccgataga tatgccgcta ctttttttga gttctttacc acttctttag
     2401 gccaagttgt cttatattgt gtgtcgaaag tagtatatgc tcaaatgaag ctatttgcaa
     2461 gcatcgcaat atgggtgtcg gttctctttt tgtggctctg tcctgcctgt aaggagacta
     2521 ccccggagaa gaaataccgc gtcggctttg cccaatgctg cgacgatcct tggcgcgatg
     2581 taatgaacag cgagatgtac cgcgagctgg aacttcatcc ggagttggaa tttgaaatgc
     2641 gcgtatccgg caacgaaagt gaaaaacagg tagaacaaat tcgggagatc gtagcgagcg
     2701 gcatagacat tttgatcgta tctcccaatg agtcaaagcc gctcacccac atcatagagg
     2761 aggtctatac atcaggaacc ccggtaattc tgattgaccg ggaaacggat tcggaattat
     2821 atacggccta cataggcgcc gataattacg aaataggaaa aacggcgggc cggtacctcg
     2881 ccaaccggct gaacggccgg ggtaagatca tagaaataca attgcccctc gcgatttcgc
     2941 cggccataga gcgcagccgg ggcttcaaag acggtattgc cgcagctccc ggcctggaaa
     3001 tcgccgcagt gctggaaacg gtaggcgggt tgaacgacat tgaagccaga tttccggata
     3061 tgctgaaacg acattccgac gtcagtgcca ttttcgggca cacggatctg ctggctgaaa
     3121 cggcttataa aatagccaaa gagcaggggc ggggaactcg tatgttcttt gtggggatag
     3181 acggcatacc cggcaccggc cgaggcatac aagcggtgga agacggtatt ctggacgcat
     3241 ccctgttgta tcctacgggc ggagcggagg ccatccggct tgccgtatcc atactcaacc
     3301 gactacctta cgaaaagcaa aataatctgg agaccatcgt catcaataaa gacaacgccc
     3361 gcattctgca caaccaaatg aagaaagttc aaagcctgca agagagcata gacgaacaaa
     3421 tgagcaatct ggccgggctg aaatctattt accgcaatca gcgggtatat attttcattc
     3481 tgttgtcgag cctcctgctg tctttggtac tgggcgtgtt tttatggcga tccttgcggg
     3541 caaaacagac cgcgcttgac agcctcgaac gcaaaaacca agaaattctg gagcatgagc
     3601 ggcaaatcat ggctatgtcg gatgaagtaa aacaggcgac gcaagccaaa gtagattttt
     3661 ttaccaatat cagccatgag tttcggacgc cgctcacgct cattttaggt tttgccgaag
     3721 acttgctgcc ctcgcccaca atcagcaaag acgtacagca gagtctggcc cacatccgac
     3781 ggaatgcctt tcgcttattg catttggtca atcaattgat ggacttccga aagtcggaaa
     3841 gcggtcaaat gaagctgcgg gctgcggaaa acgacctgat cgcgtttgta aaaaaaatca
     3901 tggagagttt tggcaatacc gcccaaaaac gaggtataga ttttcaatta ttgagccgcc
     3961 tcgagcaact cccagtatgg atagatgccg gtatgatgga caaagtactg ttcaacctgc
     4021 tttccaacgc gttcaaattt accctcgatg gaggcaaaat ttacctgacg gtcgctgtgg
     4081 acgtttttga aaaccgggca aaaatcaccg ttgaagacag cggccaaggc atacaggaag
     4141 aggatttgag gcatattttc gagccgtttt atcaggggga agagcggccg caggcaggca
     4201 ccggcctggg gcttgcgctg gctaaagcgc tggtagaact acacggcggc gccattgacg
     4261 tgcaaagcac caagggcagg ggcagtcgct tcctggtttc gttgccattg ggcaacaaac
     4321 acctgcgggc agaccaaatg acgagcgaaa ataccgctta cttatcgcac catgatttcc
     4381 tccaagcccc ggataccgac tggcaggaaa tatccggcga gatggaaaat gaaagcgccg
     4441 gccggcaaat tttgattatt gaagataatg aagaccttca gctcttcctg cgaaagaagc
     4501 tcagcgcctc ttatcaggtt ttgcaagcga cagacggcaa cgaagggctt caaaaagcgc
     4561 tggatcacac acccgacctt atcatctgcg atgtgatgct gcccggcatg aacggcatag
     4621 aaatcacccg tgcgctgaaa tccgatttgc gcagttctca tatccctgtc attatgcttt
     4681 cggcccgcag tgcgacggcg caacaaattg agggggccga ggccggtgcg gatatttatt
     4741 tgaccaaacc cttcaatatt cattttttac aagccagcat ccggtctttg ctgctcaacc
     4801 ggcaaatgct gcgcgagcat ttcagcaaag gcatttcggc tttttctact gcagatgcgc
     4861 ccgccttacc ggctgaacgg agcgccaacg ctctggatgt agattttgtg cggcagtttg
     4921 cagactatat ggaaaggcat tatgccaggc aagatttcca agtgtcagat ttatgccggg
     4981 aatttggctt gtcgaggtct cagatttaca ggaaagtaac ggcactgttg ggcgaaagca
     5041 tcagcgatta tcttcaaaat atacgcctca aaaaagcgga ggtattgctg gctgaaggcc
     5101 atttgccggt ggctgaaatc gcgtatcagg ttggatactc ctctcccgat tatttttcta
     5161 cggtattccg gtcgcgttac ggggtgcctc cgtcctcctt ccggcgggaa gcaaacacct
     5221 gaaggacggc ttttcctcaa ggtgttgatg caacaaacct acaactgcgc gcaacaatac
     5281 caaagaccgg gcagggattt tgtatttttt acaacaactt taaattattg aaaatgagca
     5341 gtttggtggc cattcggtac atgcgcgaat tttaaagaaa ctttgaacca atcctgaagc
     5401 ccggcctgtt gtgctgacaa gtacttttgt ggtgcaaaat tctgaaagta tgaatcaccg
     5461 caaaatcttt acttggtcaa ttaccgtagc gctcgcgggt tttttattcg ggttcgatac
     5521 ggtagtcatt tccggagccg acttgtcgct acagaagtta tggccccgcg gtgaagtctt
     5581 tcacggcttt gtagtcatgg cctcggcgct gtggggcacg gtagtgggag ctttgttggg
     5641 cggtattccc accgataagc tgggccgcaa aaagacgctt ttttggatag gcgttttcta
     5701 tttcgtatcg gcagtaggtt ctgccctcgc cgccgatccc ttcacctttg cttttttccg
     5761 ttttctcggc ggtctgggcg tgggcgcttc caccatagca gcgccggcct atatttccga
     5821 gatcgctcct gccaatgacc ggggcaagct ggtggctttg taccaattca acatcgtgtt
     5881 tggcatattg gtagcctttc tgagcaatta cctgctcagc ggtctgggcg aaaatgcctg
     5941 gcgttggatg gtaggcgtgg aagctgttcc ggcgcttgtt tatacggtga tggtgctggg
     6001 cgtgccccga agcccgcgtt ggttgttggt aaaaaagcac gaccgcgacg cggcggccaa
     6061 gacgcttcgg ctcatagacc ccggcatgaa tgtggaagcc gaaatggacc gtatcctttc
     6121 agatcaggtg gacgtcgtaa aaggcgaaac gatttttacg ccaaagtacc gctttgccct
     6181 gacgctggcc tttctggtcg cttttttcaa tcaattttca ggtatcaacg cctttttgta
     6241 ctacgccccc cgcattttcg agatcgccgg cctggagaaa agctccgcac tgctcagcag
     6301 cgtaggcatc ggcgtcatca acctgatatt taccctcatc ggactctcgc tgatagatcg
     6361 ctttggccgc cggcaactca tgtatatagg ctcggtgggc tatgtcatat cgctttcatt
     6421 ggtggcgctg gctttcatgc tcaatatcaa aggaatggcc gtgcctgttt ttttattcct
     6481 tttcatagct tctcatgccg ttgggcaggg tgcggtgatt tgggttttta tctctgaaat
     6541 ttttccgaac cacctgcgcg ccgccggtca ggccttcggg agttcggtac actggatact
     6601 ggctgccgtt attccctcgc tggtgcctgt gttgttcaaa agtataggcg cnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     6721 atgcccgaaa cgaaaggcat atcgctggag gcgctggagg cgcaaatggt aggaagaaga
     6781 aaataagttt catacatcac tttcgtgagt ttgaccggag ttcgggagat tccgcccgaa
     6841 cttcctttca gcattcgccg aagcaagcgg caagagtgca gggtaaggtt tgtcttgaaa
     6901 atcgtttggc atgaatacat tacgcaacac agtttttata attttagctg gatggatgct
     6961 gcattcctgt gcgctttcac ctgcgagcag acagcagtcc atgtacaaag agccacaccg
     7021 gccgcagttc catttttcac cggcagaaaa c
//
LOCUS       DAORLF010000248         7042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5785_length_7042_cov_1.440103, whole genome shotgun sequence.
ACCESSION   DAORLF010000248 DAORLF010000000
VERSION     DAORLF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_7042_cov_1.440103"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK198_17410"
     CDS             <1..276
                     /locus_tag="PK198_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HRF40576.1"
                     /translation="QYYFPGDEMADTYLTETLHAKLIRAGLDTAGVTVRFDENYTSPK
                     TKLVSYRDLKIKATFCPVIITGSPEAIAFAWQVGVGNSTGIGFGALR"
     assembly_gap    277..288
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            320..>1458
                     /locus_tag="PK198_17415"
     CDS             320..>1458
                     /locus_tag="PK198_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40577.1"
                     /translation="MIKQLTEFARRVQDEDMLALRQPLKGLHIKMRLMADTEGRVTLD
                     EHNAEVVYWNGKKEPDDFIIWTMNVIADAWMVGTNKCLDLPEKGIHSASPYCVSMKRD
                     SLEGGDKFSKDKVKITARYQRYFSNSLIFLDEETLPELGVWTRFFTEEGHVMTFLERF
                     KELYESVDMSEYVTFYLDRPMEEYSLPHQRYLADKLFNTNESKTQNPDNPLEIMGTSD
                     FFNGFNSKKPYLEHKSATFAISGRITEAEAHLLRRFQQLMGYRILPNPLPIFVSTQEA
                     EALQQELFRVAKIQFDGEHRPSHKSVIQALLENRQREGLGNYYLLYHAQGEKILDFDF
                     VPKFEYKLQRENGQPWMLYHSLFTSKVADESISNVFDFQDSVLPV"
     assembly_gap    1459..1509
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1510..2195
                     /locus_tag="PK198_17420"
     CDS             <1510..2195
                     /locus_tag="PK198_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40578.1"
                     /translation="NWFGDLGTKDSSTLFLVQTYRQTFYDFIYKSRRSAITFTVFYRI
                     MRKSIFSDLKADKIEKGYHTHERFILEKLNIWFSFYHSFSSSPQHTTDMVNHLQSLRA
                     FTVELVKKDSPVFIEQPAQFAFVSGQIIRYIFQQISSEKQPHEKLERFLRAKSGDQLK
                     SEITKLYTQYRHIKVSGRFERAMGQLMACSDEQLNADALPFMLAGYFSDNTLFADKVK
                     KEEIPNEDN"
     gene            2225..>2425
                     /locus_tag="PK198_17425"
     CDS             2225..>2425
                     /locus_tag="PK198_17425"
                     /inference="COORDINATES: protein motif:HMM:NF016963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HRF40579.1"
                     /translation="MSTPFNKRAFGCAIVKAINANYNADFTHQPRTLPDGTAYATDKA
                     LKYLVRNYWVNHIKGEHVLYYKR"
     assembly_gap    2426..2496
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2497..>3145
                     /locus_tag="PK198_17430"
     CDS             <2497..>3145
                     /locus_tag="PK198_17430"
                     /inference="COORDINATES: protein motif:HMM:NF016963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HRF40580.1"
                     /translation="EIKKEKDAKLKVLRNLLSCLDVRTFGATFANKKGDKMAISIHGP
                     LQFTHGVNRFPDSEIITEQIMSPFRDDKAGKDASDASTLGTQHKLAEGHFVHHFSLNP
                     NNLTAHLPAGATDFPSITEADVVSIKQGLRYGATYYDSAAKAGIENELLLWITLREGS
                     QVVLPSFVHLVEIGADRTIDLKKITDLLDRDRIEQEVESIEIYYDHGLTQVVNIPA"
     assembly_gap    3146..3157
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3175..>3508
                     /gene="cas5"
                     /locus_tag="PK198_17435"
     CDS             3175..>3508
                     /gene="cas5"
                     /locus_tag="PK198_17435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HRF40581.1"
                     /translation="MQTLISFDVAGDFGFFRKPDTNDGIQLSYNLIHKPSVLGILGAI
                     AGLAGYRQAGVWPQYYECLKHLRIGIEPLGEHERGNFGKVVITYTNTVGYANDRSNLI
                     VRESTLLNP"
     assembly_gap    3509..3565
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3566..3966
                     /locus_tag="PK198_17440"
     CDS             <3566..3966
                     /locus_tag="PK198_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40582.1"
                     /translation="EAQLLEYIQTGKAVYLPYFGKNECPLYWSVDDVKTYEYSDFKPE
                     SGFKIRTAFVKNKRAAAAQRKAEGAFARILEPGSFAYFEEIPIGFWEELHQYDMAEVA
                     YSNWMLSPNTDLEGLYEVVHHGDRFIIQLF"
     gene            4020..6710
                     /gene="cas3"
                     /locus_tag="PK198_17445"
     CDS             4020..6710
                     /gene="cas3"
                     /locus_tag="PK198_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HRF40583.1"
                     /translation="MPQHDHYFAHSTDGKAHKRDEWFYEHVELVNTFVERLCHAHGLD
                     AVIDAMIGAVVRSVSALEEVDGDAIKERFAYSFLFHDFGKINPNFQREKMKKWDLFPH
                     KATVPFLPDTGHSELSAYCYLRYYAPKDSSDKSWILFYLTLLLSYPIIRHHAPNLWEI
                     GAYFKDRKIVWNDKAAAFEQLRQTYTLEDGGDANLFQCWLQKFEDIQAIGEQLTKDCA
                     MELHSLIKLNFSLLTASDFLATSVYANDKDYTLYGVMDAAAKARVVVSAQQSTPYNKA
                     IFAAADDNNFVFDNPTLPGNEALNQLRLEMGVQVVRRLRNHADRRVFSIEAPTGGGKT
                     NLSMLATAELLHIKPELNKVIYVFPFTTLVTQTHKAVLRTLGLSEDEVTQLHSKAGYR
                     QMNAEGDSDGRYGDEKQDYLTYLFNHFPVTLCTHVRFFDWLTTPQKESNYGYHRLANS
                     IVVIDELQAYSPKHWDKVILFVAHFAKYFNTHFIIMSATLPKLETLLPRLGGMDVAMC
                     ALLPDAKEQYFRNPNFRHRVHFVSDYLSENMTTERLAELVVLEARQYADDHDGSVYCI
                     VECIFKRTATELFMCISDLPLFFDHVWVLSGTILEHRRRLIINRLKNKAYRQKRILLI
                     TTQVVEAGVDIDMDIGFKDQSILDSDEQLAGRINRNVNKPTCKLFLFRKDRASILYKN
                     DLRYQISRDELRPSEITRILQEKDFDALYNPVIDRIVRGNESTLNPGHKAYLYELEKL
                     DFQKIQREFRLIENDNLSLFVPLCLPLCVEDEQGQMNDVLISEADAVFLRRHGIAVTN
                     MVNGEDVFDLFLRMVNKQMPDEGYIRNKTDFIRLRGVMSKFIFSMFASDKFQNKIMPF
                     LDMEKSENGYLYLSQWQDIYDEKTGLSGAALDDVTHQIIG"
     gene            6710..>7042
                     /locus_tag="PK198_17450"
     CDS             6710..>7042
                     /locus_tag="PK198_17450"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HRF40584.1"
                     /translation="MNLPTATQINLYHVCHRQLWLHSHEIRMEQESDMVYEGKLIGEN
                     TYARRSNQWREVDLGHIKIDHYDPRNKIVKEVKKSNKLEAAYIAQVKYYIYYLERNGE
                     QDVTGLIEY"
BASE COUNT         1978 a         1542 c         1638 g         1681 t
ORIGIN      
        1 cagtattact tccccggcga cgaaatggca gacacctatc tgacggaaac gctgcacgcg
       61 aaattgatcc gcgccggact cgatacggca ggcgtaacgg tacggttcga cgagaactac
      121 acctcgccca aaacgaaatt agtcagctac cgcgacctga aaataaaggc caccttctgc
      181 cccgtcatca tcactggcag ccccgaagcc atcgcatttg catggcaagt gggtgtggga
      241 aactcgacgg ggattggttt tggggcacta aggtgannnn nnnnnnnngg tgattgagaa
      301 ctaaataaac tgaataaaga tgataaaaca actgacagaa tttgcccggc gtgttcagga
      361 tgaagacatg ctggcgttgc ggcagccgct gaaggggctg catattaaaa tgagattaat
      421 ggccgatacg gaaggccgag tgacgctgga cgaacataat gcggaggtgg tctattggaa
      481 tggcaagaaa gaacccgatg actttattat atggactatg aatgtcatag ccgatgcttg
      541 gatggtggga acgaacaagt gtttagatct tccagaaaaa ggcattcatt ccgcatcgcc
      601 ttactgcgta agtatgaagc gcgactcttt ggaaggaggg gataagtttt ccaaagacaa
      661 agtaaaaatt acagcgcggt accaacgcta ctttagcaat tcattgattt ttttggacga
      721 ggagacactt cccgagttgg gtgtttggac gcgctttttc acggaagaag ggcatgtgat
      781 gacatttttg gagcggttta aggaattgta cgagagcgtg gatatgagcg aatacgtcac
      841 tttttactta gatcgcccca tggaggagta tagcttgcct catcagcgtt accttgcaga
      901 taaactgttc aacaccaatg aatccaaaac acaaaaccca gacaacccgt tagagataat
      961 gggtacgagc gacttcttca atgggtttaa ttccaaaaag ccttacttgg agcataagtc
     1021 cgccaccttt gccatcagcg gacggattac agaagccgaa gcgcacttgt tgcgccgatt
     1081 tcagcagttg atgggttaca ggatactgcc caatccgctg cccatatttg taagtaccca
     1141 ggaagctgaa gcattgcagc aggaactgtt cagggttgca aaaattcaat ttgatgggga
     1201 gcatagaccc agccacaaat ctgtcattca ggctttattg gaaaatagac aacgtgaagg
     1261 cttgggcaac tactatctac tctaccatgc ccaaggggaa aaaatattag attttgactt
     1321 cgtgcctaaa tttgaataca agttgcagcg tgaaaatggg cagccgtgga tgctgtatca
     1381 ttctttgttc acgtctaaag tagctgatga aagcatatct aatgttttcg attttcagga
     1441 ttcggtattg cctgtcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnna taattggttc ggagatttag gtacaaagga cagtagtacg ctgtttttgg
     1561 tgcaaacata ccgacagaca ttctacgatt ttatctacaa aagcaggcgt tcagccatta
     1621 cgttcacggt gttctaccgc atcatgcgaa aaagtatttt ttctgacttg aaagcggata
     1681 aaatagaaaa gggatatcat acacatgagc gattcatctt ggaaaagctc aatatctggt
     1741 tcagctttta tcattcattt tcttcatcac cacaacatac aacggatatg gttaatcatc
     1801 ttcaatcttt gcgtgcgttc actgtcgaat tggtaaaaaa agacagccct gtgttcatcg
     1861 aacaacccgc gcagttcgct tttgtgagcg gacagattat tcggtacatt tttcaacaga
     1921 ttagctccga aaagcaaccg catgaaaaac tggaacgctt cctccgtgcc aaatcgggcg
     1981 accaattgaa aagtgaaatc acgaaactat atacccaata ccgccacatc aaagtgagcg
     2041 gccgcttcga acgggccatg gggcaattga tggcctgttc ggacgaacag ttgaatgcag
     2101 acgcgctgcc ctttatgttg gcggggtact tctcagataa tacacttttt gccgacaagg
     2161 taaaaaaaga ggaaatacca aacgaagaca actaattttc aaaactttat aaacacattt
     2221 gaatatgtca actccattta acaaacgcgc ctttggctgc gccatcgtca aagccatcaa
     2281 cgccaactac aacgcggact tcacgcacca acctcgcacg ctgcccgacg gaacggccta
     2341 cgctaccgat aaagccttga aatatttggt gcgcaattat tgggtcaacc acatcaaggg
     2401 agagcatgtg ctgtattaca aacggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaga ttaaaaaaga aaaggatgca
     2521 aagctgaagg tgctgcgcaa cctactttct tgtctggatg tcagaacgtt tggagctaca
     2581 tttgctaata aaaaagggga caaaatggct atttccatcc acggcccctt gcaattcact
     2641 catggtgtta accgctttcc cgattccgaa attattacgg agcaaatcat gtccccattt
     2701 cgggatgaca aggcaggaaa agatgcatcg gatgcctcca cccttggcac gcaacacaag
     2761 ctggccgaag ggcattttgt acaccacttt tcattgaacc ccaacaacct aactgcgcac
     2821 ttgcccgccg gagctacgga ttttccgagc atcacggagg cagatgtggt gagcatcaag
     2881 caaggactac gctacggcgc tacctactac gacagtgcag ccaaggcggg aattgaaaat
     2941 gagctcctgt tgtggattac cctgcgggag gggtcgcaag tggtattgcc ttcgtttgtc
     3001 catttggtag aaatcggtgc tgaccgcacg attgatctca aaaaaatcac cgatttgtta
     3061 gatcgcgacc gtatcgaaca ggaagtggaa agcatcgaaa tttactacga tcacggattg
     3121 acccaagtag tcaatattcc cgccgnnnnn nnnnnnntat gaaatttcgt agctatgcag
     3181 acactgatct catttgacgt ggcgggagat tttggcttct tccgcaagcc ggacaccaac
     3241 gacggcatcc aactgagtta caacctcatc cacaagcctt cggtattggg tattttgggg
     3301 gccattgcag gtttggcggg ttacagacag gcaggcgtct ggccacaata ttacgagtgc
     3361 ctcaaacacc ttcgcatcgg gatagaaccc ctcggcgaac atgagcgcgg caatttcggt
     3421 aaagtggtca tcacatacac gaacacagtg gggtacgcca atgaccggag caacctcatc
     3481 gtgcgcgaaa gtaccctgct taatccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnntggaa gcacaattac tggaatacat ccaaacagga
     3601 aaggctgttt acttgcccta ttttggcaag aacgaatgcc cgttgtattg gtctgttgat
     3661 gatgtaaaaa cctatgagta tagcgatttc aaacccgaat cgggttttaa aattcgtacg
     3721 gcattcgtta aaaacaaaag agcagccgca gcacagcgaa aagcggaggg agcctttgca
     3781 cggatattgg agcctggtag tttcgcctat ttcgaggaaa ttcccatcgg attttgggag
     3841 gaattgcatc agtatgatat ggcagaagtt gcctatagca actggatgtt atcaccgaat
     3901 accgatttag aaggtcttta tgaagtagtc catcatggcg accgtttcat tatccaacta
     3961 ttctaatcat gcggacgata gcccaaatat ttgcagacgc acccagcata caaaacatct
     4021 tgcctcagca cgatcactat tttgctcaca gcacagacgg caaggcgcac aaacgagatg
     4081 aatggttcta tgaacatgta gaactggtca atacatttgt ggaaaggttg tgccacgcgc
     4141 atggattgga tgcagtgata gatgcaatga taggggctgt cgtccgttcc gtatctgcat
     4201 tggaagaagt ggatggagat gccatcaaag agcggtttgc ttatagtttt ctgttccatg
     4261 actttggaaa aatcaacccc aatttccaac gggagaagat gaaaaaatgg gacttgttcc
     4321 cccataaagc caccgttcct tttctgccgg atacgggaca ctctgaattg agcgcgtatt
     4381 gttatctgcg ctattatgca ccgaaggatt cgtctgataa atcctggatt ttgttttatc
     4441 ttacgttgtt gctgtcgtat ccgattattc gtcaccatgc gcccaacctg tgggaaatag
     4501 gggcatactt taaggatcga aaaatcgtat ggaatgacaa agctgccgcg ttcgagcaac
     4561 tcagacaaac ttatacgctg gaagacggcg gcgatgcaaa tttatttcag tgctggttgc
     4621 aaaaatttga agatattcag gcaataggcg agcaattgac aaaggattgt gcgatggaac
     4681 tgcacagctt aatcaaactg aatttttcct tattgacagc atccgatttt ttggccacca
     4741 gtgtctatgc caacgacaaa gactatacac tttacggcgt gatggatgcg gctgcgaagg
     4801 cacgagtagt tgtatccgcc cagcaatcaa caccctacaa caaggcgata tttgcggcag
     4861 cggacgacaa caacttcgtg tttgataatc ctaccctgcc gggcaacgaa gcgctcaatc
     4921 aattgcggtt ggagatgggg gtgcaggttg tacgccggct ccgcaaccat gccgaccggc
     4981 gcgtattctc catagaagca cctactggag ggggaaaaac caacctgtcc atgctggcaa
     5041 ctgcggaact gcttcacata aagccggaac tgaataaagt catctatgta ttccctttca
     5101 cgacattagt gacgcagacc cacaaggcgg tgttgcggac attgggcttg tcggaagatg
     5161 aagtgacgca attgcactcc aaggcagggt ataggcaaat gaatgcagag ggagatagtg
     5221 atggtcgtta tggcgatgaa aaacaagact atttgaccta cctgttcaac cactttcctg
     5281 taacactttg cacccatgtc cgtttttttg attggctgac aaccccacaa aaggaatcca
     5341 actatggata tcatcgttta gccaacagca ttgtcgtcat tgatgaattg caagcctact
     5401 cgcctaaaca ttgggataag gtcatattgt ttgtggcaca tttcgccaaa tattttaata
     5461 cccactttat tatcatgtcg gcgacgctgc ccaaactgga gacgttgctg ccccggctag
     5521 gggggatgga cgtggcgatg tgtgcgctgc tccctgatgc caaagaacag tattttcgca
     5581 accccaactt ccggcaccgg gtacattttg taagcgatta cctgtcagaa aacatgacga
     5641 cagagcggct ggctgaactt gtagtattgg aagcgcggca gtatgccgat gaccatgacg
     5701 gcagcgtcta ttgtattgta gagtgtatct tcaaacggac cgcaaccgag ttgtttatgt
     5761 gcatttcaga cctgccgcta ttctttgacc atgtatgggt attgagcggc accattttgg
     5821 aacaccgccg gcggctcatt atcaaccgat tgaaaaacaa ggcatatcgc caaaaacgca
     5881 tcttgctcat tactacccaa gttgtggaag cgggtgtgga cattgatatg gatatcggct
     5941 tcaaagacca atctattcta gatagcgatg agcagttagc cgggcgtatc aatagaaatg
     6001 tgaataagcc cacatgcaag ttattcctgt tccgaaaaga cagggccagt atcttgtaca
     6061 aaaacgacct tcgttatcaa atcagcagag acgaattgcg ccccagcgaa attacccgga
     6121 ttctacaaga aaaagatttt gatgccctgt acaatccagt tattgaccgc atcgtacgtg
     6181 gcaacgaaag cacactcaat ccgggacaca aagcatacct gtatgaatta gaaaagttgg
     6241 actttcaaaa aatacagcgt gaatttcgcc tcattgagaa cgataacctc agtttgtttg
     6301 tgccgttgtg cctgcccttg tgtgtggaag atgaacaggg acaaatgaat gatgtactga
     6361 tatcggaggc agacgctgtc ttcctgcgaa ggcacggaat agcagtaacg aatatggtga
     6421 atggcgagga cgtgtttgat ctgtttctgc gaatggtcaa taaacaaatg ccggatgagg
     6481 gctatatccg caacaaaacc gatttcatac gactgcgcgg tgtaatgtcg aaattcattt
     6541 tttcgatgtt tgcatccgat aagtttcaaa acaagattat gccattttta gatatggaaa
     6601 aaagcgagaa cggctacctg tatctatcgc aatggcagga tatttatgac gaaaagacgg
     6661 gcctgtctgg tgctgcattg gatgatgtta cacaccaaat cattggctga tgaacctacc
     6721 taccgctact caaatcaacc tgtaccacgt ctgccaccgg cagctctggc tacacagcca
     6781 cgaaatacgc atggagcaag agtctgacat ggtctatgaa ggaaaactca ttggcgagaa
     6841 cacctatgca cgccgatcta accaatggcg ggaagttgac ttggggcata tcaagattga
     6901 ccattacgat ccgcgcaaca aaatcgtaaa ggaagtaaag aagagcaaca aattagaagc
     6961 ggcctacata gctcaggtca aatattacat ttactacctc gaacgcaacg gggaacaaga
     7021 tgtgacagga ctgattgagt ac
//
LOCUS       DAORLF010000249         7040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5787_length_7040_cov_1.717394, whole genome shotgun sequence.
ACCESSION   DAORLF010000249 DAORLF010000000
VERSION     DAORLF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_7040_cov_1.717394"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..384
                     /locus_tag="PK198_17455"
     CDS             136..384
                     /locus_tag="PK198_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40585.1"
                     /translation="MNRILTFCCCLCLCGVALAQSGTVQIGFPGQLSGDFEYLNALPP
                     GGWDLDSNGVFDIGRPCSCRPMPPIQNGSSNNAGVFDW"
     gene            378..>752
                     /locus_tag="PK198_17460"
     CDS             378..>752
                     /locus_tag="PK198_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40586.1"
                     /translation="MVVATGVSGQTWRIQYADLALNPASLLPLPFGAIVPEVGNTGVY
                     ALHFAHRDAARYAFVLENTADFPGQSFGPFINTCYYPDPELYYLDDFYCDNSPGVLLY
                     GSATSPFDENLLPVDPTDEIWTI"
     assembly_gap    753..999
                     /estimated_length=247
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1000..>1318
                     /locus_tag="PK198_17465"
     CDS             <1000..>1318
                     /locus_tag="PK198_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40587.1"
                     /translation="PAMLLTFTPITYRGYRVDVLDPFGNPLGTVIPADYAFVPLLGVL
                     TDECTGLYCTTQITARDVHAPVLTVPANRNISCAETPDTTTTGRATAVDCSPVQITYT
                     DQTA"
     assembly_gap    1319..1445
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1446..>3840
                     /locus_tag="PK198_17470"
     CDS             <1446..>3840
                     /locus_tag="PK198_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRF40588.1"
                     /translation="DLFLDCAQYAADPSLTDPTPGKAGIPNLVDIPNCGLIYAHSDDT
                     LGLCGNTQLNFVILRTWLVLDACGFQVFNIDGLGNDNIQFIRVSDETPPVITAPLARM
                     SAALSPLQTGLPECTSAGFIPPPQVSDACNNFQVRIFTPLGEAIYVNGSNGASGAHVP
                     FPGLPLGQHPITYRATDVCGNQSEFQGLIEVVDSLPPLMLCNNSINLTLSGTGTARVM
                     PNVIDAGSRDDCCIGERLLRLAGEPESAFRSFVDVTCPAVPSQQVVLRVSDCFGNFNE
                     CEATVHWVDPIPISVVSAPGPVAVFCQDDLTPYGNAAFQAPVFTDNCPFETTFQVQTN
                     LNNCGIGTVQRTWTAGGAATVSQTVTVNGRFEYSFRLPNDLMAQCGAATVPDDVAILA
                     QSCDQFVFTIRTDTITAGAPGHCTSIQRTIDWVNLCQTAAGTPPLTLPRQGGLDAGKG
                     YDMRVQNGMVFQVAFDGSLLPIGPATGSYRYVQRWDVLDSAPPVFSFTAPPVFCTQTA
                     CNGEATFLFAASDNCAPALGIIRRVSFNGQSPMPDPGLLTALGNGQYRIVGQYPAGLH
                     TLEIVLSDGCGNITTGVIPFEVRDCTLPVLVCRQDLVLSLETGGILELRPDLFPLQLT
                     DNCPGGLLSFQPLALDTIRLMTCDSLGFRTVNIWLTDAAGNQAACQSNVEVLAGAGVC
                     PEFWNISGMVGNENQLPVSGVSIRLSGHIEATEITPMSGQYEFFDLPGQRDYTLRPEK
                     NINVSNGVSTFDLVLISRHILGVEALGSPYKIIAADVNRSGTVSTLDLILIRRVILGA
                     "
     assembly_gap    3841..3868
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4177..4647
                     /locus_tag="PK198_17475"
     CDS             4177..4647
                     /locus_tag="PK198_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HRF40589.1"
                     /translation="MHWRTLDGRRIEIPIKDAVEQAIIRETSMGHRLKVCIGSDSQVR
                     SGHVEFASVIVFLREKKGGFMYILNDRLDREISIRERMIMEVAKSVEIAYALCDLLDR
                     YHVDLEVHADINTDPHFQSNVALKEAMGYILGMGFAFKAKPDAFASSSCADKVV"
     gene            4737..5618
                     /locus_tag="PK198_17480"
     CDS             4737..5618
                     /locus_tag="PK198_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40590.1"
                     /translation="MHPSLLLPLLLLSMPLLAQQQRGRLPAQLAEASGLHIAAPDSLW
                     WHNDSGGKASLFLTDGKGRLLADLPVPHTLNNDWEDLTTDPQGRFYIGDFGNNANRRQ
                     DLRIYIFHPAAQTTDSILFSYPDQTAFPPPPHQAAFDMEAFFWWNDTLHLFSKNRLVA
                     GNYLPRHYTLPPGQYTATLIDSMLLPRRVVTAATISPDGSQVALLSYLFRWVLGFIPV
                     TRTTVFLWEDFPGSRFLQGRSQAVRVRKCLFPTQYEAIDYRPDGALLIASESVPFRKA
                     HFRTVKPKKVSVTAPRP"
     gene            5708..6373
                     /locus_tag="PK198_17485"
     CDS             5708..6373
                     /locus_tag="PK198_17485"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF40591.1"
                     /translation="MKTFSILVAAAALLLTLPAWAQSSWKTISGEGPVVKETLSLDDF
                     HSIGLGISGIVYVQPGKTQKVIVEAQKNLIDNLKQEVKNGSWNIGFHNNVRNYSDVKI
                     YITIPTVQALSIGGSGKIIATEAFNNLGDLHFSIGGSGNIEFSGDANSLKVSIGGSGN
                     VKAANLRVGSSKISIGGSGNCYVEVGDRLDVSIAGSGSVHYKGRPKINSSIAGSGKVV
                     TLD"
     gene            6592..>7040
                     /locus_tag="PK198_17490"
     CDS             6592..>7040
                     /locus_tag="PK198_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRF40592.1"
                     /translation="MSKMYASLTGVHCFVPDDVLTNHDLAQMVDTNDEWIMTRTGIRE
                     RHILKGEGQGVSVMGIEAVKGLLEKTNTDPSEIDLVICATVTPDMMFPDTANLIAHAT
                     DMKNAFCFDLAAACSGFIFALSTASTYIQSGMAKKVVVIGADKMSSI"
BASE COUNT         1382 a         2063 c         1688 g         1505 t
ORIGIN      
        1 gccatttccc ttcatgtagg tgtgtgttgc cacgcagttg ccgttcatcc ggcggcaagt
       61 ctgtgtcata ccggtccgat ggagaccggg gttagtgtgt agttttattt gtgtcccaaa
      121 aacgatatac cacccatgaa cagaatcctt actttttgtt gctgcctgtg cttgtgcggg
      181 gtggctctgg cccaatccgg tactgtgcag atcggctttc ccggccagct ttccggcgat
      241 tttgaatacc tcaatgccct gcctcccgga ggttgggact tagacagcaa tggcgttttc
      301 gacatcgggc gcccctgctc ttgccgaccg atgccgccca ttcagaacgg cagttccaat
      361 aacgccggcg tgttcgattg gtagtagcca ccggtgtgag tggccaaaca tggcgcattc
      421 agtacgccga cttagccttg aacccggcct cactactgcc gttgccgttt ggcgctatcg
      481 tgccggaagt aggtaatacc ggcgtttatg cgctccactt tgcccaccgg gatgccgccc
      541 gatacgcttt tgtgttggaa aacacagccg attttcccgg ccagagcttt ggacctttta
      601 ttaatacttg ttattacccc gatcctgagt tgtactatct ggatgacttt tattgcgaca
      661 acagccccgg cgtactgctt tacggttcgg ccacgtcgcc ttttgatgaa aatctgttgc
      721 cggtggaccc gacagatgaa atctggacca tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccgccatgct gctcaccttc
     1021 acgcccatca cttacagagg ctaccgcgtg gacgtgctcg atcccttcgg caatccgctg
     1081 ggcacggtca ttcctgccga ttacgcattc gttcctttgc ttggcgtact caccgatgaa
     1141 tgcacgggcc tgtattgtac cacgcaaatc accgcccgcg atgtgcatgc gccagtactc
     1201 acagtacccg ccaaccgaaa catctcctgc gccgaaacac ccgataccac taccactggc
     1261 agagctacgg cggtggactg ctcgccggtg caaattacct acaccgacca gacggcccnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnncgacc tgtttctcga ttgtgcgcaa tacgccgccg atccaagcct gaccgacccg
     1501 acgcccggca aggcaggtat tcccaatttg gtggacattc ccaactgcgg cctcatttac
     1561 gcccacagcg acgatacgct gggattgtgt ggcaatacac aactcaactt cgtcatactg
     1621 cgcacttggt tggtgttgga tgcctgtggt tttcaggtgt ttaacataga cgggctgggc
     1681 aatgacaaca tccagtttat tcgcgtcagc gatgaaacgc cgccggtcat caccgcgcca
     1741 ctggcccgaa tgagcgccgc gctctcgccc ttgcaaacgg gccttccgga gtgtacctca
     1801 gcgggattta ttccaccgcc acaggtttcc gatgcctgca ataactttca ggtgcgcatt
     1861 tttacgccgc tcggcgaggc catctacgtc aatggcagca acggggcgtc gggcgctcat
     1921 gtgccgtttc ccgggctgcc tctgggccaa cacccgatca cttacagagc gaccgacgtt
     1981 tgtggcaatc agtctgaatt tcagggactt attgaagtgg tggacagcct gccaccgctc
     2041 atgttgtgca acaacagcat caatctcacc ctttcgggaa ccggcaccgc ccgcgtgatg
     2101 ccgaatgtga ttgacgccgg cagccgcgac gattgttgca tcggggaacg cctacttcga
     2161 ttggccggtg agccggaatc agcgtttcga tcctttgtgg acgttacctg tcctgctgtg
     2221 ccttcccaac aggtagtatt gcgcgtaagc gactgtttcg gcaatttcaa cgaatgcgag
     2281 gccactgtac attgggtgga cccgattccc atctcagtgg tgtccgcgcc ggggccggtg
     2341 gctgtttttt gtcaggatga cctgacgcct tacggcaatg ccgcttttca ggcgccggtc
     2401 tttacagaca actgcccttt tgaaaccact tttcaggtgc aaaccaacct caataactgc
     2461 ggtatcggca ccgtccaacg cacatggaca gcggggggcg cggccaccgt gagccaaacc
     2521 gtgacggtga acggccgctt tgaatacagc ttccgcctgc ccaacgacct gatggcacaa
     2581 tgtggtgctg ccactgttcc cgacgatgtg gccatcctcg ctcaatcctg cgatcaattt
     2641 gttttcacca ttcgtacaga caccatcact gccggcgcgc cggggcattg caccagcata
     2701 caacgaacga tagactgggt gaacctttgc cagactgccg ccggaacgcc gcccctgacc
     2761 ctgccgcgac aaggcggact ggatgcaggc aaaggctatg atatgcgggt gcaaaacggc
     2821 atggtttttc aggtggcttt tgatggcagt ctccttccta tcgggccggc taccggttcg
     2881 tatcgctatg tgcaacgctg ggatgtactc gactctgcgc cgccggtttt cagtttcaca
     2941 gcgccgcctg ttttttgcac ccaaacggcc tgcaacggcg aagctacgtt cctttttgcg
     3001 gcaagcgaca actgcgctcc tgcgctcggc atcattcggc gagtatcctt caacgggcag
     3061 tcgccgatgc ccgatcccgg cctgctgacg gcattgggca atgggcagta ccgcattgtg
     3121 gggcagtatc cggcaggcct tcatacgttg gaaatcgtgt tgagtgatgg ttgcggcaat
     3181 attactaccg gcgtcattcc ttttgaggtg cgcgactgca cgctgccggt gttggtgtgc
     3241 cggcaagacc tcgtgttgtc attggaaaca ggcggtattt tagaactccg ccccgatttg
     3301 ttccccttac aactgaccga taactgcccc ggcggcctgc tttcgtttca gccgctggca
     3361 ttggatacca tccggctgat gacctgcgat tcgctgggtt tcagaaccgt caacatctgg
     3421 ctgaccgatg cggcgggcaa tcaggcggcc tgccagtcga acgtggaagt gctggccgga
     3481 gcgggcgtgt gtcctgaatt ttggaacatc agcggcatgg tcggaaatga aaaccagttg
     3541 ccggtatcag gtgtaagcat ccgactgtcc ggtcatattg aggcgacgga aatcacgccc
     3601 atgtcggggc aatatgagtt tttcgatttg cctggccaac gcgattatac attgcggccc
     3661 gaaaagaaca tcaacgtgtc gaacggcgta tccactttcg atctggtact catcagccgc
     3721 catattctgg gggtggaggc cctgggatcg ccctacaaaa tcatcgccgc agacgtgaat
     3781 cgctccggta cggtttctac gctggatttg attttgatac ggcgggtcat tctgggggcc
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac aacaactcct ggcggttcat tcccgccgat
     3901 tttgaatttt ccatatccgg caatccgttt ttcggggccg gtttcccgga agaggtggtt
     3961 ttgccgcagc ttgggagaga tacggcggtg cattttattg ccatcaaggt gggagatgtg
     4021 aacggcagcg ccaatccaca gcagtaggcg ggaactaaag tgcatacaag cgcggttggg
     4081 aggctttgta aaacaaaagt aaattcggag ttaatatttc gatgaatgct tttgtgtgtc
     4141 aaagattttt ccctactttc gtaaaaccgt tgaattatgc actggagaac attggacgga
     4201 cggcgcatcg aaattcccat caaagacgcc gtagaacaag ccatcatcag ggaaaccagc
     4261 atgggccatc gcctcaaggt gtgcatcggc tccgactcgc aagtgcggag cggccacgtt
     4321 gaattcgctt cggtgatcgt gtttttgcgc gagaaaaaag gcggcttcat gtacatcctc
     4381 aacgaccgct tagaccgcga aatcagcatc cgcgagcgca tgattatgga ggtggccaag
     4441 tcggtggaaa ttgcttatgc cctctgcgac ctgctggacc gctaccatgt ggacctggag
     4501 gtacacgccg acatcaacac cgatccgcac ttccaatcga atgtggcgct caaggaagcc
     4561 atgggctaca tcctgggcat gggttttgcg ttcaaagcca agccggatgc ctttgccagc
     4621 tcgtcttgcg cggataaggt ggtgtgaggt aggggcaact ctggtagggg caaccacgag
     4681 ggttggccct acctaccggc ctctctctca atcattacaa aaaaatccac tccgtcatgc
     4741 acccgtctct cctcctgcca ctgctcctcc tttctatgcc cctccttgcc caacagcagc
     4801 gcggccgcct gcccgcgcaa ctcgccgaag catccggcct gcacatcgcc gcacccgaca
     4861 gcctctggtg gcacaacgac agcggcggca aagcctccct cttcctcacc gacggcaaag
     4921 gccggctgct cgccgacctg cccgtgcctc acaccctcaa caacgactgg gaagacctga
     4981 ctaccgaccc ccagggccgt ttttacatcg gcgatttcgg caacaacgcc aaccgccgcc
     5041 aagacctccg catctacatc ttccatcctg ccgcccaaac caccgacagc atcctctttt
     5101 cttatcccga ccaaaccgcc ttccccccgc cgccgcatca ggccgccttc gatatggaag
     5161 ccttcttttg gtggaatgac accctgcatc tgttttccaa aaaccgcctc gtagccggca
     5221 attacctccc ccgacactac accctgcccc ccggccagta caccgccact ctgatagaca
     5281 gcatgctgct gccccgacgc gtagtcaccg ccgccactat cagccccgac ggctcgcagg
     5341 tggccctgct cagctatctt ttccggtggg tactgggctt cattcccgtc acccgcacca
     5401 ctgttttttt gtgggaagat tttccgggca gccgcttttt gcagggccgc agccaggctg
     5461 tccgcgttcg aaaatgtttg tttcccacac agtacgaggc cattgactac cgccccgacg
     5521 gcgccctgct catcgcctcc gaaagcgtgc ctttccgaaa agcgcatttc agaaccgtga
     5581 agccaaagaa agtatcagtg acagcgcccc ggccctgaat gttgcctcca cttgttcgtc
     5641 gcctatttcc gttcacatgc aaccccgccg ctccaatctt gtcattacct ccaaaaaaat
     5701 caaaaacatg aaaacattca gcatcctcgt cgccgcagcg gccctcctcc tcacactgcc
     5761 tgcatgggcg caatcttcct ggaaaaccat ctccggcgaa ggccccgtcg tcaaagaaac
     5821 actctcgctc gacgatttcc acagcattgg cctcggcatc tccggcatcg tgtatgtgca
     5881 gcccggcaaa acgcagaaag taatcgtcga agctcagaaa aacctgatag acaacctcaa
     5941 gcaggaagtc aaaaacggct cctggaacat cggtttccac aacaacgtcc gcaattattc
     6001 cgacgtcaaa atttacatca ccatccccac cgtacaggcg ctatccatcg gcggcagcgg
     6061 caaaatcatt gccaccgaag ccttcaataa cctcggcgac ctccacttct ccatcggcgg
     6121 cagcggcaac attgagttct ccggcgatgc caactccctc aaagtgagca tcggcggcag
     6181 cggcaatgtc aaagcagcca accttcgcgt aggcagcagc aaaatcagca ttggcggcag
     6241 cggcaactgc tacgtcgaag tcggcgaccg ccttgatgtc tccatcgccg gcagcggcag
     6301 cgtacactac aaaggccgcc ccaaaatcaa ttccagcatc gccggttcgg gcaaggtggt
     6361 gacattagat taaggcgttc ctctttttct tttttgggcg ccccccgcca caggcaactg
     6421 acataccggc ggggccgggc cgtccactct catagcccct tgcacgcata ccatccggcg
     6481 gcaaggggct atgccgtttc cgtccctgtc gcaacgcatt ccattttttt tcttgtgtct
     6541 tggcataact ttctaccttc gcgccgattt ttgcaacacg caactacgtc tatgtctaaa
     6601 atgtacgctt cccttaccgg ggttcactgt tttgtgcccg atgatgtgct caccaaccat
     6661 gacctcgcac aaatggtgga caccaacgac gaatggatta tgacccgcac cggcatacgc
     6721 gagcgccaca tcctcaaagg agaaggccag ggcgtttccg tcatgggcat tgaggccgtc
     6781 aaaggcttgc tcgaaaagac caacaccgac ccctccgaaa ttgacctcgt catctgcgcc
     6841 accgtcaccc ccgacatgat gtttcccgat acggccaacc tcattgcgca cgccaccgac
     6901 atgaaaaatg ccttctgttt cgacctcgcc gctgcctgct ccgggtttat ttttgccctc
     6961 agcacggcct ccacttatat tcagtcgggc atggccaaaa aggttgtcgt catcggcgcc
     7021 gacaaaatgt catccatcat
//
LOCUS       DAORLF010000250         7028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5799_length_7028_cov_1.936899, whole genome shotgun sequence.
ACCESSION   DAORLF010000250 DAORLF010000000
VERSION     DAORLF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_7028_cov_1.936899"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PK198_17495"
     CDS             complement(<1..765)
                     /locus_tag="PK198_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40593.1"
                     /translation="MFRSCLLVLATLTLAASAQAATFIVDTTSDAMLADCDDGVAADC
                     SLRGAIALASANPDADTVAFDIPSSDPGYISATAHWRIEVSSGAFPFIQYPLTIDGYT
                     QAGATANTQAPDEGGSNAVLKIEFSGANAWSNGLHGSQMTVRGLAINRFRGGNLNLAG
                     PGPHRIEGCFIGTGITGMTAPGDNASNIGIRVSSGNVVVGGDTPGARNVISGNSYIGI
                     SDSGGTSSPPNTYQGNILGLAADGSSLIAGRQDFGIY"
     gene            complement(917..>1420)
                     /locus_tag="PK198_17500"
     CDS             complement(917..>1420)
                     /locus_tag="PK198_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40594.1"
                     /translation="TANADAFSATGINFISPDFAGANDTCFVDTGLNTTASAYSYRVR
                     FYSASNLLGASEPASSVRLSIVPTDRANQLSWTEMVPWDNYRYTIFRKNTAGSFDSLS
                     TVIKSPYTDGGLVNGQEYCYLVRSEGTYGVTFRITNGLGQYTEVTASLPIHAGETPLF
                     KDGFEGP"
     assembly_gap    1421..1466
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1467..>2683)
                     /locus_tag="PK198_17505"
     CDS             complement(<1467..>2683)
                     /locus_tag="PK198_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40595.1"
                     /translation="FPNPQFQGCNNTPRLLQPPVDIGCVGRAFRHNPNAYDADGDSLS
                     YHFTVPRQDIGENVPNYRFPSEINPGPNNQLTINERTGDILWQSPARAGEYNLAIIIV
                     SYRNGVPLDTIIRDMQILILECDNLPPVVETTIDEICVIAGQLVEIPVRATAPIVESN
                     QRVRLTALGGPFEVAVSSAVFEPLNNTFQTQPVLKTFRWQTTCEHISGQYYSVVFKAT
                     DNFLGDSSGLATLKTVRIKVVGPPPTGMAVTSGQGRAEVTWDKPYVCDAAADNYFRGF
                     TIWRREGSNIFPIDTCTPGLEGKGYTKLTQAPFKTEQNGRYYFLDENVDRGRTYCYRV
                     VAEFAKTTPAGQYVYNRVESLPSDEVCVQLSRDIPLITHVDVQRTDAVTGEIRVCWSK
                     PVAGDLDTLLNPGP"
     assembly_gap    2684..2695
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2696..3142)
                     /locus_tag="PK198_17510"
     CDS             complement(<2696..3142)
                     /locus_tag="PK198_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40596.1"
                     /translation="MMPKSSSFRSFLFVLGIFAALPLLATHNRAGEISIEQVGDCVTS
                     LTIKATITTYTKASSIQADRDTLTICWGDGQCERVARSNGGGVPPRGVVIENDTKYNT
                     YIAFHTYPARSTYVISMTDPLRNAGILNVNFPNSDQIKFHLATTYTF"
     gene            complement(3269..3832)
                     /gene="pth"
                     /locus_tag="PK198_17515"
     CDS             complement(3269..3832)
                     /gene="pth"
                     /locus_tag="PK198_17515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRF40597.1"
                     /translation="MKYLITGLGNIGSEYADTRHNIGFMVVDALARAAEAKWETEQLG
                     DVARIRHKGRIFVLLKPSTYMNRSGKSVRYWLQKEKIEQSNLLVVLDDLNLPFGKQRL
                     RGQGSDGGHNGLKDIDAILGNNNYARLRIGIGDEFAKGRQSDFVLSPWSDAENEQLPE
                     ILKLASDIVKSFGAIGLNQTMNLYNKK"
     gene            complement(3846..>5052)
                     /locus_tag="PK198_17520"
     CDS             complement(3846..>5052)
                     /locus_tag="PK198_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF40598.1"
                     /translation="GDKIIGVGDSIIAGKKLVNKDITSLLRGEIGTKVQLLIKRGSGK
                     PFKVSVVRDKIPMHSMDAALMLTEKTGYIRLNRFSATTHNEFMKALEKLHTKQGLEHL
                     VIDLRQNPGGYLQQAINILSQLFPAKGVPLVHTQGRAVSRSDYETAGRVLFPVKEIVV
                     LIDEGSASASEILAGALQDQDRAAIVGRRSFGKGLVQEQYELRDGSALRLTVARYYTP
                     SGRSIQKPYDNLSDYDHETEDRLESGELTAATAALPEDSVKYYTSKGRVVFGGGGITP
                     DYYVPLDTALLNDYYLRLRPHLQSFVFNYSQKNKTQFEGLKLKEFKNTFTVKDQIIND
                     FIAYTAGKGVAKEAQQLPLIMPELRRQLKAQLARTLFQDEGFFAIMTAEDPMVKKALS
                     VIEGKQAQR"
     assembly_gap    5053..5062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5063..5531)
                     /locus_tag="PK198_17525"
     CDS             complement(<5063..5531)
                     /locus_tag="PK198_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF40599.1"
                     /translation="MHSETPRPGNGRIFIWLPFLLSAVLAVGILTGMRLQSAAPPLVI
                     NGEPGADPSAPAGYGKLEELLRYVEAKYVDPVDREKLVDEAIESILQQLDPHSSYIPS
                     DQVEIVEEHMEGSFSGIGVEFLMMEDTLTVIAPLGGGPAEKAGIKAGDIILSVD"
     gene            complement(5634..>6657)
                     /locus_tag="PK198_17530"
     CDS             complement(5634..>6657)
                     /locus_tag="PK198_17530"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HRF40600.1"
                     /translation="MATEGYERPRNRIYELTNYLTANQINVEHRYFGASVPDSLDYRY
                     LNLRQATADLHHIRTLLGELYRKAWVSTGISKGGQTTIFYRYFYPDDVAASVPYVAPL
                     NLDLADKRIYSFLDTVGPEPCRRAIRDVQIRLLKERDKVLPLLRWHAKGAGESFTYLS
                     LEEAFEYAVLEYSFSFWQMGFDCNQIPVAKTADLEALLEHFIEVSGLSFFADASMKNY
                     ASHYWQAGAEMGYYAYQTAPFKGLLKSLSGEPSAIFMPGKAPKTFEPTLVKTVYDWLQ
                     NKGHRFIYIYGGTDTWTATGVPPSDKVDALWFVMPGKNHGSARIANMSTDERTRLLDQ
                     LKKWME"
     assembly_gap    6658..6792
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1400 a         1764 c         2067 g         1594 t
ORIGIN      
        1 gtagatgccg aaatcctgac gccccgctat caggctgctg ccgtcggccg ccaaaccaag
       61 gatgttgccc tgataggtgt tgggcgggga actggtgccg ccggaatccg atatcccgat
      121 gtagctgttt ccggagatca cattgcgcgc gcccggggta tcgccgccca ccacgacatt
      181 gccggaggac accctgatgc cgatattgct cgcattgtct cccggcgccg tcatgccggt
      241 gatgccggtt ccgatgaagc agccttcgat gcggtgcgga cccggaccgg ccagattcag
      301 attgccgccg cggaagcggt tgatcgccaa gccgcgcacc gtcatttgcg agccatgcaa
      361 tccattgctc caggcattgg cgccggagaa ttcgatcttg agcaccgcat tgctgccgcc
      421 ctcgtccggc gcctgcgtgt tggcggtggc gcccgcctgg gtgtacccgt cgatggtgag
      481 cgggtactgg atgaatggaa atgcgccgct ggacacctcg atgcgccagt gcgcggtggc
      541 gctgatgtag cccggatcgg aggacggaat gtcgaatgcg acggtgtccg catcggggtt
      601 ggcgctggca agggcgatgg caccccgcag cgaacaatcc gcggcgacgc catcgtcgca
      661 gtcggcaagc attgcatcgg acgtggtgtc gacgatgaac gtggccgcct gcgcactggc
      721 agccagcgtc agcgtggcga ggacgagaag acacgaacga aacatgggga agcctccttg
      781 acgtggcgat ccgatgccac tgcttcccca ttcaatacgt cgtctgcgcg gagacctgac
      841 acggatcagg ttctcaggcc ggatcgaaac cccatccatc cttcggatgc cagcccctgc
      901 ttgcaccgag caggcgctac ggtccttcaa acccatcctt gaacaggggc gtttctccag
      961 cgtgaatggg aagggaggcg gtgacctcgg tgtactgacc aaggccattg gtgatgcgga
     1021 acgtcacgcc ataggtgccc tcgctgcgta ccaaataaca gtactcctgg ccattgacga
     1081 ggccgccatc tgtgtaaggg ctttttatca ccgtggacag agagtcgaaa ctgccggctg
     1141 tgttttttct aaaaatggtg tagcggtagt tgtcccaggg caccatttcc gtccacgaca
     1201 actggttggc cctgtcggtg ggtacgatgc tgagtcgtac ggaagaagct ggttcggagg
     1261 cgccgagtag gttggatgcc gagtaaaaac gaacgcgata gctgtaggcc gaggcggtgg
     1321 tgttcaggcc tgtgtccaca aaacaagtat cgttggcgcc ggcaaaatca gggctgatga
     1381 aattgatgcc tgtggcgctg aacgcatccg cattggctgt nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnggcc cgggattgag cagggtgtca aggtcaccgg
     1501 ccacgggttt ggaccagcac acccgtattt cgcccgtcac ggcgtcggtg cgttgcacgt
     1561 ccacatgagt gatgagtggg atgtcgcggc tgagttgtac gcatacttca tcggagggca
     1621 ggctctctac ccggttataa acatactggc cggcgggggt ggttttggca aattcggcca
     1681 ccacccgata gcagtaggtc cggccgcggt ccacattttc atccaaaaag taataccgcc
     1741 cgttttgttc ggttttaaag ggcgcctggg tgagtttggt ataacctttg ccttcgaggc
     1801 cgggcgtgca ggtgtctatc ggaaaaatat tgctgccctc tctgcgccat attgtgaagc
     1861 ccctgaagta gttgtcggcg gcggcgtcgc agacgtaggg cttgtcccag gtgacttctg
     1921 ccctgccttg tcccgaagtg acggccatgc cggtgggcgg cggccccact actttgatgc
     1981 gcacggtttt gagcgtggcc aagcctgaac tgtcgcccag aaagttgtcg gtggctttaa
     2041 aaacgaccga gtagtattgg ccggaaatgt gttcgcaggt ggtttgccaa cggaatgttt
     2101 tcagtacggg ctgcgtctga aaggtattgt tgaggggttc aaaaacggcc gaactgacgg
     2161 ccacctcaaa cgggccaccg agcgccgtga ggcgcaccct ttggttggac tcgacgatgg
     2221 gcgcggtggc gcgtacggga atttcaacca actgaccggc aatgacacaa atctcgtcta
     2281 tcgtagtttc taccacgggc ggcaggttgt cgcactccag aatgagaatc tgcatgtcgc
     2341 ggatgatggt gtccaaaggt acgccgttgc ggtaggatac gatgatgatg gccaagttgt
     2401 actcgccggc gcgggcgggc gattgccaca gaatatcgcc ggtgcgctcg ttgatggtga
     2461 gctggttgtt ggggccgggg ttgatttcgc tgggaaagcg ataattgggt acgttttcgc
     2521 cgatgtcttg ccggggcacg gtaaagtggt aggaaagact gtcgccgtcg gcgtcgtagg
     2581 cattgggatt gtgccggaag gcgcgcccca cgcagcctat gtccaccggc ggctggagca
     2641 gacgcggtgt gttgttgcaa ccctgaaact gcggattggg aaannnnnnn nnnnnaaaag
     2701 tataagtggt ggctaagtgg aatttgattt gatcggaatt gggaaaattg acattgagga
     2761 tgcctgcatt gcgcaagggg tcggtcatgg aaatgacgta agtgctgcgg gccgggtagg
     2821 tgtggaacgc tatgtatgta ttgtatttgg tatcgttttc gatgacgacg cccctcggag
     2881 gaacgccgcc gccattggag cgcgccacgc gttcgcactg gccgtcgccc cagcagatgg
     2941 tgagcgtatc gcggtcggcc tgtatgctgc tggctttggt gtaggtagtg atggtcgctt
     3001 tgatggtgag actggtgacg caatcgccta cctgttctat cgaaatttcg ccggcgcgat
     3061 tgtgcgtagc caagaggggc aaggcagcaa aaatgcccaa tacaaacaga aacgaacgga
     3121 aggaagaaga tttcggcatc atctgtggca ttgtgaggaa gatccaaagc aatattgccg
     3181 gtgagatcta tcttggcaaa aaacgcctca tcaggggtaa aggttctgac aggtacatga
     3241 cacaaacctg tgccgcgctc agccgggatt acttcttgtt gtacaggttc atggtctggt
     3301 tcaggccgat ggcgccaaaa ctttttacga tgtcggaggc gagtttcaaa atttccggca
     3361 gttgttcgtt ttccgcatcg ctccaggggc tgagtacaaa atcagactgg cgccctttgg
     3421 caaactcatc gccgatgccg atgcgaagcc gggcgtagtt gttgttgccc aaaatggcgt
     3481 caatgtcttt aaggccgttg tgtccgccgt cgctgccctg ccctcggagg cgttgtttgc
     3541 cgaaaggcag attcaaatca tcgagaacga cgagcaggtt gctttgctca atcttctctt
     3601 tttgaagcca gtagcgcacc gatttcccgc tgcgattcat ataagtggaa ggcttgagca
     3661 gcacgaaaat gcggcctttg tgcctgatgc gcgccacgtc gcccaattgc tcggtttccc
     3721 atttcgcctc ggcggcccgc gccagtgcgt ccaccaccat gaagcctatg ttgtggcggg
     3781 tgtcggcgta ttcggagcct atgtttccca atccggtgat aaggtatttc attgtttcct
     3841 gatacttatc gctgtgcctg ctttccttca ataacgctca atgccttttt taccataggg
     3901 tcttctgcgg tcataatggc gaaaaaacct tcgtcctgaa acaaggttcg cgccaattgg
     3961 gccttcagtt gccggcgcaa ttcgggcatg atgagcggca attgctgggc ttcttttgcc
     4021 acgcctttac cggcagtata ggcaatgaag tcattgatta tctggtcttt aacggtgaaa
     4081 gtgtttttaa attccttcag ttttaaaccc tcgaattgcg ttttgttttt ttgagaataa
     4141 ttaaaaacaa aagactgcaa gtgcggcctc agccggaggt aataatcatt gagcagggcc
     4201 gtgtctaaag gcacataata atcaggcgtg atgccgccgc cgccaaacac cacgcggcct
     4261 ttgctggtgt aatatttgac ggaatcctcc ggtaaagcgg ctgttgcagc agtcagttcg
     4321 cccgattcga ggcggtcttc ggtttcgtgg tcgtagtcgg aaagattatc gtaaggcttc
     4381 tgaatagagc gccccgacgg cgtgtagtaa cgggctaccg tgaggcgcag cgctgagccg
     4441 tcgcggagtt catactgctc ttgtaccaag ccttttccaa acgaacgacg ccccacgatg
     4501 gcggccctgt cctgatcctg taaagcgcct gcgaggattt cgctggccga ggccgaacct
     4561 tcgtcaatga gcaccacaat ttctttgact ggaaacagca cgcgcccggc ggtctcatag
     4621 tcggagcggc tcacggcccg gccctgcgta tgcaccaagg gcacgccttt ggccggaaac
     4681 aactggctga gaatgttgat ggcctgctgc agatagccgc ccggattctg gcgcaggtca
     4741 atgaccaagt gctccagacc ttgcttggta tgcagttttt ccaatgcttt catgaactcg
     4801 ttgtgcgtgg tggcgctgaa acggttgagc cggatatagc ccgttttttc ggtcagcatc
     4861 agggctgcat ccatgctgtg catagggatt ttatcgcgta ctacgctcac tttgaagggt
     4921 ttgcctgagc ctcgtttgat gagtagttgc actttggtgc cgatttcgcc gcgcagcaaa
     4981 gaggtgatgt ctttgttgac taatttcttt ccggcaatga tggaatcgcc cacgcctatg
     5041 attttgtcgc cgnnnnnnnn nnaatccacg ctgagaataa tatcgccggc tttaatgcct
     5101 gccttttcag ccgggcctcc gcccaatgga gcgatgacgg tgagggtgtc ttccatcatc
     5161 aaaaactcca cgccgatgcc gctgaaactg ccttccatgt gctcttccac gatttctacc
     5221 tgatcggagg ggatgtagct ggaatgcggg tcgagctgct gcaaaatact ttcaatggct
     5281 tcgtccacca atttttcgcg gtccaccggg tccacatatt tggcttctac atagcgcagc
     5341 aattcctcca acttgccgta gcctgcggga gcggaagggt cggctccggg ttcgccgttg
     5401 atgaccaaag gcggggccgc cgattggagg cgcatacctg tgaggatgcc gacagccaat
     5461 accgccgaca gcaaaaaagg cagccagatg aagatgcggc cattgcctgg ccggggagtt
     5521 tcggagtgca tattcgtaaa agtattgcca agcaacaacg acaagcgttc ggtagtttag
     5581 ggagacgggc tatttgcgcc tctgccgaac aatgcttctg tgttttttga agcctattcc
     5641 atccattttt tcaactgatc cagcagccgg gtgcgctcgt ctgtgctcat gttggcgatg
     5701 cgcgccgagc cgtggttttt gccgggcatc acaaaccaca gcgcgtccac cttgtcggaa
     5761 ggaggcacgc cggtagccgt ccaggtatcg gtgccgccgt agatgtagat gaagcgatgc
     5821 cccttgtttt gcagccagtc atacacggtt ttcaccaaag tcggttcaaa ggttttgggc
     5881 gctttaccgg gcataaaaat ggcgctgggt tcgcccgaca gcgacttgag caagcccttg
     5941 aacggcgcgg tttggtaggc gtagtaaccc atttcagcgc cggcctgcca atagtgcgag
     6001 gcgtagtttt tcatagacgc atcggcaaaa aacgacaggc cggaaacctc aataaagtgc
     6061 tccagcaggg cttccagatc ggcggttttg gcgacgggaa tttggttgca atcaaagccc
     6121 atttgccaaa aagagaacga gtattccagc acggcgtatt caaaagcctc ttccagagaa
     6181 aggtaagtga agctctctcc ggcgcccttg gcgtgccagc gcagcagggg caacactttg
     6241 tcgcgctctt tgagcaggcg tatttgtaca tcgcggatgg cgcggcggca aggctccggc
     6301 ccaacggtgt cgaggaagga atagatgcgc ttgtcggcca gatcgaggtt gagcggggcc
     6361 acatacggca cagaggcagc cacgtcgtcg ggataaaaat agcggtaaaa aatggtggtt
     6421 tggccgccct tgctgatgcc ggtgctcacc catgctttgc ggtagagttc gcccaacagc
     6481 gtgcggatgt ggtgcaggtc ggcggtggcc tggcggaggt tgaggtagcg gtagtccaag
     6541 ctgtcgggca cggaagcgcc gaaatagcgg tgctctacat ttatctgatt ggccgtgagg
     6601 tagttggtga gttcgtagat gcgattgcgg ggccgctcgt agccttcggt ggccatcnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nngtggccga ttccattttt ttaaacgata catcgggcag gttgaacagc
     6841 gctttttcca aggcggccgg ctgcgaccag gcgctgattg ccacgaatac aaaaaggatt
     6901 gtcagtgtca aaaatctgtt tttcatattt cgagtatttt gttgattcag aagttgttca
     6961 aggtttcggg tttcatccgc cgtagcctta gcgaaggcgg gttcaaggtt tcgggttgca
     7021 tccgccgt
//
LOCUS       DAORLF010000251         7022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5802_length_7022_cov_1.981771, whole genome shotgun sequence.
ACCESSION   DAORLF010000251 DAORLF010000000
VERSION     DAORLF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_7022_cov_1.981771"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK198_17535"
     CDS             complement(<1..420)
                     /locus_tag="PK198_17535"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40601.1"
                     /translation="MTGRISFLLNFTVFATLFLLSCQGPPQTVTADDDLARFVNPFVG
                     TGAHGHTYPGAALPFGMVQLSPDTRLDGWDGCSGYHFTDTIIYGFSHTHLSGTGVSDY
                     GDVLLMPFTGDPLFHNGADGQAGYSSAFDKSSESASPG"
     gene            520..>2033
                     /locus_tag="PK198_17540"
     CDS             520..>2033
                     /locus_tag="PK198_17540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRF40602.1"
                     /translation="MKYLVHLAAFLFLQTSIAQTFLVQPFLQNAAPNAVTITWESSTA
                     ATGTVEWGLSTSLGQSTAGQAQTGSGVSQIFHARLTGLSPDTRYFYRVNIGTTMSSIF
                     DFVTPPTNDSEKSFSFIAMSDMQRDPGRPNVFREMCNDGVIGYLGQTGTADIAGRIAF
                     AMITGDLVDYGADYQQWKNTYFNPSENLFRHTPMYPVLGNHEANSINYFKYFELPTNG
                     TTGYEEHWWYKDYANVRIIGLDSNTGYLIQAQLNWLENVLNDACSNEHIDFVFAQIHH
                     PFHSELWIAGNLAYTGQVIQKLENFSAACGKPSVHFYGHTHGYSRGQSLNAAHTMVNV
                     ATAGGNIDYWNEYAQNDYPEHVISQDEYGFAWIDVRAGNDPAFTIRRISRGNENVFMN
                     NVLRDSFTIRRYNAGPATPTGVFPTEGLVVSSGCTLELSATPYSDPDGDLHGASQWQI
                     STSPDFSNPTVDRWKQYTNWYNNVDLQANDDLTDELASNLAPNTTYYWRVRYRDR"
     assembly_gap    2034..2169
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2170..2873
                     /locus_tag="PK198_17545"
     CDS             <2170..2873
                     /locus_tag="PK198_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40603.1"
                     /translation="FESIFSGECAGNNAYAGSRLFAVGGVCEEHPYAEGFQEVSVEAF
                     AAAIQNGTAIARFGGWLSDYQGTDLPQFRLRFLSAAGTVLDSTPVYGSQSGVWELVQA
                     SAVIPQDCSRIRFVLMGTRQAGQDNDSYFDEMFLRVDTVGCNGIISAATEVQQDAALF
                     RVFPNPASSEISIEAALWRENVRFEVSDAAGRTVMAGTMASQIQRVDVSGLSKGLYFI
                     RFLGKADKPLKFIKH"
     assembly_gap    3045..3075
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3219..5150
                     /locus_tag="PK198_17550"
     CDS             3219..5150
                     /locus_tag="PK198_17550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRF40604.1"
                     /translation="MSQFIVSARKYRPARFEDVVGQEHISKTLKNALQTGHLAHAFLF
                     CGPRGVGKTTCARILAKVINCQNAGTTFEPCNTCDSCKAFNENASFNITELDAASNNS
                     VDAMRALIDQVRFQPQQGKYKVFIIDEVHMLSSQAFNAFLKTLEEPPSYAIFILATTE
                     KHKIIPTILSRCQIYDFKRISAQDAAKHLQHICSQEGIEADEAALHIIGQKADGALRD
                     ALSIFDRMVAAGGNRITYEAVLENLNVLDYDYYYRFVDGLLSEDLPGVLLLFDEVVRK
                     GFDPDIFILGLAEHLRNLLVCKDEATLSLLEVSDNLRERYRTQAHLAPAALLLSALNL
                     ANECDLNYKMARNKRLHTEMALIKMSYIRRRVRYSPVETAGDPEKKNPDVRVDGERLA
                     VGGLNPKPETRNLELGTRNHASAVASPESETRISANDANFREREGNPEPETRNELAVG
                     GGQLAVGSSNPELETQNIEPTFAKATADETRNQPAPTRKHTVAKLGSLESMLEDQLKV
                     SDHKEASYAPDFNVESLKKSWMQYANGVPSDSSKTFLRQVELGIENEHITVTVNSALA
                     ESTLKQEMGVIEFLRTHFRRGGLTLSIKVEKKAQADDEPVPRATPLNDKEKLRQMQQS
                     NPHLRTLWERLGLGIEGDG"
     gene            complement(5211..6665)
                     /gene="icd"
                     /locus_tag="PK198_17555"
     CDS             complement(5211..6665)
                     /gene="icd"
                     /locus_tag="PK198_17555"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HRF40605.1"
                     /translation="MKFKMSGTKVSIANGKLVVPNDPILPFIEGDGTGPDIWAASVRV
                     FDAAVEKAYGGTRKIHWKEVLAGEKAFNQTGNWLPDETLAAFSEYLVGIKGPLTTPVG
                     GGIRSLNVALRQILDLYVCLRPVRWFKGVPSPVKRPEDVDMVIFRENTEDIYAGIEYA
                     GGSPEAQKVLDFLATEFPKEFNKIRFGTEQKSKDWQAELEKIGMAKRDVGVNVGIGFK
                     PTSWLGTERLVHSAISYALTNNRRNLTLVHKGNIMKFTEGAFREWGYQIGTQFFRDKV
                     VTERESWILGNVESNQGISAEDNARMIDPGYDMMTPEQKKKVVAEVETALQLLPTHGN
                     GQWKKMLMIKDAIADITLQQVLTRAKDFDVIATMNLNGDYLSDALAAQVGGIGIAPGA
                     NINYITGHAIFEATHGTAPKYAGQDKVNPGSVILSGEMMLRYMGWTEAADLIIKGMDG
                     AIGNKRVTYDFHRLMDGATLLKCSEFGSEIIANM"
     gene            6742..>7022
                     /locus_tag="PK198_17560"
     CDS             6742..>7022
                     /locus_tag="PK198_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF40606.1"
                     /translation="MPNPNFDPKEERLQNDEGQVEKALRPKALDDFSGQPRIVENLRI
                     FIAAAKQRGEALDHVLLNGPPGLGKTTLSHIISNELGSGLKMSSGPVIE"
BASE COUNT         1641 a         1809 c         1850 g         1555 t
ORIGIN      
        1 accgggcgaa gccgattcgg aagatttgtc gaaagcggag gagtagccgg cctgaccgtc
       61 ggcgccattg tgaaacagcg gatcgccggt aaaaggcatc agcagcacat cgccataatc
      121 ggaaacgccg gtgccgctga ggtgcgtgtg gctgaatcca tatataatgg tgtctgtgaa
      181 atggtagccg gagcagccgt cccagccgtc gaggcgggta tcgggactga gttggaccat
      241 gccgaatggg agcgccgcgc cggggtaagt gtgtccgtgt gcgccggtgc cgacaaaggg
      301 attgacgaaa cgggcgaggt cgtcatcggc agtcactgtc tgcggcgggc cttggcagga
      361 aagcaggaag agcgtggcga atacggtgaa attcaataaa aaagagattc tacctgtcat
      421 ggttggcgtg cgttggatga tgatttttcg gatgacaata ttacgccaaa gcgggcgcga
      481 ttgactacat ttggactcat tttttcaatc actcaccaca tgaagtacct cgtccacctt
      541 gcagcgttct tgtttttgca aacttccatc gctcaaacct ttctggttca gccgtttttg
      601 caaaatgccg cccccaacgc cgtcaccatc acctgggaat cctcgacggc tgcgacaggc
      661 acggtggaat ggggtctgag taccagtttg gggcaaagca ctgccgggca ggctcaaacc
      721 ggatcgggcg tctcgcaaat tttccatgcc cgcttgaccg gcctctcgcc cgatacgcgc
      781 tatttctatc gcgtgaatat tggtacgacg atgagttcca tcttcgattt tgtgacgcca
      841 ccgacgaatg attctgaaaa atcgttctcg ttcatcgcca tgagcgacat gcaaagagac
      901 cccggccggc ccaatgtttt cagggaaatg tgcaacgacg gcgtcatcgg ttatctgggg
      961 caaaccggca cggcggacat tgccggaagg attgcatttg ctatgattac cggcgatttg
     1021 gtggattacg gcgccgatta ccagcaatgg aaaaacacct atttcaaccc ctccgaaaac
     1081 ctgtttcggc acacgcccat gtacccggtg ttgggcaatc acgaggccaa ctcaatcaat
     1141 tatttcaaat attttgaact cccgaccaac ggaaccacag ggtacgaaga gcattggtgg
     1201 tacaaagact acgccaatgt tcgcatcatc gggctggatt ccaacacggg ctacctcata
     1261 caggcgcaac tgaactggtt ggaaaacgtg ctgaacgacg catgttccaa cgagcacatt
     1321 gattttgttt ttgctcaaat ccaccaccct tttcattcgg agttgtggat tgccggaaat
     1381 ctggcctaca ccgggcaggt gattcaaaaa ttggaaaatt tctctgccgc ctgcggcaaa
     1441 cccagcgtgc atttttacgg ccatacgcac ggttattcga gggggcaaag cctcaacgcc
     1501 gcacacacta tggtcaatgt ggcaacggcg ggcggcaata ttgattattg gaacgaatat
     1561 gcccaaaacg actacccgga gcatgtcatc agccaggacg aatacggttt tgcatggatt
     1621 gatgtgcggg ccggcaacga tcccgctttt accatccggc gcatcagccg gggcaatgaa
     1681 aatgtgttta tgaacaatgt gctgagagac agcttcacca tacggcgcta caacgcgggg
     1741 cccgctacgc ccacaggcgt gttcccgacc gagggattgg tcgtatcttc gggctgcacg
     1801 cttgagttgt cggctactcc ctattccgac cccgacggcg acctgcacgg cgcaagtcag
     1861 tggcaaattt ctacctcgcc cgatttttcc aacccaacgg ttgatcgttg gaaacagtac
     1921 accaattggt acaacaacgt tgatttacag gccaatgacg acctgacgga tgaattggcg
     1981 agcaatctgg cgcccaatac cacctactat tggcgggtga gataccggga tcgnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnc ttttgaatcc atcttcagcg gggaatgtgc cggcaacaac gcctacgccg
     2221 gcagccgttt gtttgcggta ggcggcgtgt gtgaggagca tccctacgcg gagggatttc
     2281 aggaggtgtc ggtagaggca tttgccgcag ccatccaaaa cggcacggcg attgcgcgtt
     2341 tcggtggctg gttgagcgat tatcagggca cagatttgcc tcagttcagg ctgagatttt
     2401 tgtcggcagc gggcacagtg ctggactcaa cgccggtgta cggttctcaa tcgggcgtgt
     2461 gggaattggt gcaagcatcg gcagtcattc cgcaagattg ttcgcgcatc cggtttgtac
     2521 tcatgggcac ccggcaggca ggacaggaca acgattctta ttttgatgaa atgtttttgc
     2581 gcgtagatac agtgggctgt aacggcatca tttcggctgc taccgaggtg caacaagatg
     2641 ccgcgctgtt tcgcgtattt cccaacccgg cttcttctga aatcagtata gaagcagcct
     2701 tgtggcgaga aaatgttcgt tttgaagttt cagatgccgc aggccgtacc gtgatggcgg
     2761 gaacaatggc ttcacagatt cagcgcgtgg acgtatccgg cttgagcaag ggcttgtact
     2821 tcatcaggtt tttaggcaaa gcagacaagc ctttgaagtt tatcaaacac tgagccggga
     2881 gatttcattt tcggggtgtg ataactccaa ctgaaatact atctttggac ggtaaaatcg
     2941 cacatcggag ctaaagtctc tgatactcaa aggtaacaag tatttactta gcatacctca
     3001 aaagctgcga gccacgagct gtgagctatg agtttgtttt tttgnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnagtta gattttgcat tgggggaaat tgcaaaatct actattgaaa
     3121 atgagtgcgt ttttgggaag ttttcccttt cacaatcatt ctactcaaag ctcgaagccg
     3181 gcaactcgca gcctgagagt acaaatgttg acaaaaacat gagccagttt atcgtctctg
     3241 cccgtaaata ccgacctgcg cgattcgagg acgtcgtggg gcaggagcat atttccaaaa
     3301 ccctgaaaaa cgccctccaa acggggcatc tggcgcatgc gtttttgttt tgcgggccgc
     3361 gcggcgtggg caaaaccacc tgtgcgcgca tcttggccaa agtcatcaat tgccaaaatg
     3421 caggcacgac ttttgaaccc tgcaacacct gcgattcctg caaagccttc aacgaaaacg
     3481 catctttcaa catcaccgaa ctcgacgccg cttccaacaa ctcggtggat gccatgcgcg
     3541 ccctcataga tcaggtgagg tttcagccgc aacagggcaa gtacaaggtt ttcatcatag
     3601 acgaggtaca catgttgtcc tcgcaggcgt tcaacgcgtt cctcaaaaca ctggaggagc
     3661 cgccctctta cgccattttt attctggcca ctaccgaaaa gcacaagatc attcccacta
     3721 tcctttcgcg gtgccagata tacgatttta aacgcatttc ggcgcaggat gccgccaaac
     3781 acttgcagca tatatgcagt caggaaggca ttgaggccga cgaggcggcg ctgcacatca
     3841 tcggccaaaa ggcagatggc gccctgcgcg acgcactctc catttttgac cgcatggtgg
     3901 ccgcgggcgg caatcgcatc acttacgagg cggtattgga gaacctgaac gtgctggact
     3961 acgactacta ttacaggttt gtggacggcc tgctttcgga agacctgccg ggggtgttgc
     4021 tcctgtttga cgaggtggtg agaaagggtt ttgatcctga tatttttatt ctgggattgg
     4081 ccgaacattt gcgcaacttg ctggtttgca aggatgaagc tacgctctcg ctgctcgaag
     4141 tgtccgacaa tctccgagag cgataccgca cgcaggcgca cctggctccg gcggcgctcc
     4201 tgctcagcgc gctcaatctg gccaacgaat gcgacctcaa ctacaaaatg gcgcgcaata
     4261 agcgcctgca cacggaaatg gcgctcatca aaatgagtta catccgccgc cgcgtccgtt
     4321 attcgccggt agaaacagcc ggtgacccgg aaaaaaaaaa tcctgatgta agggtagacg
     4381 gtgagcggtt ggcagtgggc ggtctcaacc cgaaacccga aacccgaaac ctggaactcg
     4441 gaactcgaaa ccacgcctcc gccgtggcaa gcccggaatc ggaaacgcga atttccgcga
     4501 atgacgcgaa tttccgcgaa cgcgagggaa acccggaacc cgaaacgcgg aatgagttgg
     4561 cagtaggcgg tggacagttg gcggtaggca gttccaaccc ggaacttgaa acgcagaaca
     4621 ttgaacccac cttcgccaag gctacggcgg atgaaacccg aaaccagccc gctccaaccc
     4681 gaaaacacac cgtagccaaa ttgggtagtc tggaatccat gctggaagac caactcaagg
     4741 tttccgatca caaagaagcc tcgtatgcgc ccgattttaa tgtggagtca ttgaaaaaat
     4801 cctggatgca atatgcgaac ggcgttcctt ccgattcgtc caagactttt ctccgacagg
     4861 tggagcttgg cattgaaaat gagcacatta cggtcacagt gaacagcgcg ctggctgaaa
     4921 gtaccctcaa gcaggaaatg ggcgtgattg agtttttgcg cactcatttt cggcgaggcg
     4981 gtctcaccct cagcatcaaa gtggaaaaga aagcgcaagc cgacgacgag cctgtgccca
     5041 gagcaacgcc cctcaacgat aaagaaaaac tgcgccagat gcagcaaagc aacccgcatc
     5101 tgagaacgct gtgggagcgg ctggggctgg gaattgaagg ggatgggtga caaaaaaagc
     5161 gccacagacg ctctcgttgt tgaattcgat gggaattgaa cccctgaaaa ttacatattt
     5221 gcaatgatct cagagccgaa ctccgagcat ttcagcagcg tagcgccgtc catcaggcga
     5281 tggaaatcgt aggtcacgcg tttgttgccg atggcgccat ccataccttt gatgatgagg
     5341 tcggcggctt ccgtccaacc catgtagcgc agcatcattt cgccggaaag gatgaccgac
     5401 cccggattca ctttgtcctg gcctgcatac ttgggcgcgg tgccatgcgt agcctcaaag
     5461 atggcgtggc cggtgatgta gttgatattg gcgccgggtg caatgccgat gccgcccacc
     5521 tgcgccgcga gggcgtcgga gaggtagtcg ccgttgaggt tcatggtagc aatcacgtca
     5581 aaatctttgg cgcgggtcag cacctgctgc aaagtgatgt cggcaatggc gtcctttatc
     5641 atcagcattt ttttccactg accgttgccg tgcgtgggca gcaattgcaa ggcagtttcc
     5701 acctcggcca ccaccttttt cttttgctcc ggcgtcatca tgtcatagcc cggatcaatc
     5761 atgcgggcat tgtcttccgc cgagatgcct tggttggatt ccacattgcc caaaatccag
     5821 gactcgcgct ccgtcaccac tttatcgcgg aaaaactgcg taccgatctg atagccccac
     5881 tcgcggaaag cgccctcggt gaacttcatg atattgcctt tgtgtaccag cgtcaggttg
     5941 cggcggttgt tggtcagcgc gtaagaaatg gccgagtgta ccaagcgttc ggtacccaac
     6001 caggaagtcg gcttgaagcc gatgcccaca ttgacgccca catcgcgctt ggccatgccg
     6061 attttttcca attcagcctg ccaatctttt gatttctgct cggtaccgaa gcggattttg
     6121 ttgaactctt taggaaattc tgttgccaaa aagtccagca ccttttgcgc ttcaggactg
     6181 cctcctgcat attctatacc ggcgtaaatg tcttctgtgt tttcgcggaa aatgaccata
     6241 tccacatctt cggggcgctt caccggagat ggcacgcctt tgaaccagcg caccgggcgc
     6301 aggcaaacgt agaggtcgag tatctggcgc aaagctacgt tgaggcttcg gatgccgccg
     6361 cccacggggg ttgtcagcgg gcctttgatg ccgacgagat attcgctgaa agccgccagt
     6421 gtttcatcgg gcagccagtt gccggtttgg ttgaaggctt tttcaccggc caatacttct
     6481 ttccagtgaa tcttgcgcgt gccgccataa gctttttcta cggcagcatc aaaaacgcgc
     6541 accgaagccg cccaaatatc ggggccggtg ccgtctcctt cgatgaaagg aagaatggga
     6601 tcgttgggaa cgaccaattt tccgttggca atgcttactt ttgttccact cattttaaat
     6661 ttgattttat tgacatcggg acgccccgaa tatgtcagga tgaaaagctg ttttcgagcg
     6721 gcgcaaaagt acggacagat aatgccaaat ccaaattttg atccaaaaga agaacgcctg
     6781 caaaatgatg aaggacaggt agaaaaagcc ttgcgcccca aagccttaga cgacttttcc
     6841 ggtcagccgc gcatcgtcga aaacctgcgc atttttatag cggcagccaa gcagcgcggc
     6901 gaggcgctcg accacgtttt gctcaacggc cctccgggat tgggcaaaac cacgctctcg
     6961 catattattt ccaatgagtt ggggtccggc ctgaaaatgt cctcaggtcc ggtcatcgaa
     7021 aa
//
LOCUS       DAORLF010000252         7008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5811_length_7008_cov_2.139652, whole genome shotgun sequence.
ACCESSION   DAORLF010000252 DAORLF010000000
VERSION     DAORLF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_7008_cov_2.139652"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    290..324
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(381..>849)
                     /locus_tag="PK198_17565"
     CDS             complement(381..>849)
                     /locus_tag="PK198_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRF40607.1"
                     /translation="HPAAIRRLLQLTRLPDATQTELLVWSLEALKQLTAHLHPRLETL
                     LYFHQRPLTVVAPGADHLPEEALHPDGSVALRIVRDSFCVGLLQAFGGPVLSAPAVTE
                     GAPYPATFGGISSEIIQGCNHVVRHRQSERSPGEPSVMVRLSEETEELEFLRE"
     assembly_gap    850..877
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1409..1783
                     /gene="rpsL"
                     /locus_tag="PK198_17570"
     CDS             1409..1783
                     /gene="rpsL"
                     /locus_tag="PK198_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRF40608.1"
                     /translation="MPTINQLVRHGREKVVVKSKSRALDACPQKRGVCTKVYTTTPKK
                     PNSALRKVAKVRLTNQIEVIAYIPGEGHNLQEHSIVLVAGGRVKDLPGVRYTIVRGSL
                     DTQGVNNRKKSRSLYGTKRPKK"
     gene            1814..2284
                     /gene="rpsG"
                     /locus_tag="PK198_17575"
     CDS             1814..2284
                     /gene="rpsG"
                     /locus_tag="PK198_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRF40609.1"
                     /translation="MRKRKPKVRVIAQDPRYTDPMITQFVNNLMWDGKKSTAFELFYN
                     AMDLVAQRTSTDDVHAVWKKALNNVMPQVEVRSRRIGGATFQIPTEIRAKRKVSIGMK
                     WLIKFARQRSGKGMADRLAAEIVAASKGEGAAVKRKEDMHKMAEANRAFAHFKV"
     gene            2329..4527
                     /locus_tag="PK198_17580"
     CDS             2329..4527
                     /locus_tag="PK198_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRF40610.1"
                     /translation="MSKDLKFTRNIGIAAHIDAGKTTTTERVLYYTGLTHKIGEVHEG
                     AATMDWMEQEQERGITITSAATKTAWRWKEEEYTVNIIDTPGHVDFTVEVNRSLRVLD
                     GLVFLFCAVSGVEPQSETNWRLADNYKVPRIGFVNKMDRSGADFFSVVNDVRTKLGSN
                     AIPLQVPIGAEEKFRGVVDLITNEAIIWNEHDKGMTYEVIPIPEDLKETVTEWRTKLV
                     EEVDIPASEKERAMEMHNALVEAAAENEEGLMEKFFEDPNSISAEEMRAAIRAAVMDM
                     KFVPMMCGSSFKNKGVQAVLDAVCAFLPSPTDVDAIKGVNPKTDQEEIRKPSVTEPFA
                     ALAFKIATDPYVGRLAFMRVYSGALDAGSYVYNSRSDSKERISRLYQMHANKQNAIDR
                     VEAGDIAAAVGFKDIRTGDTLCDLDKPIVLESMVFPEPVIAIAVEPKSQKELDKLGMA
                     LAKLAEEDPTFRVKYDEDTNQTVISGMGELHLEIIVDRLRREFKVECNQGAPQVNYKE
                     ALTGTVSHREKLKKQTGGSGLFADMEFELGPADAEFLETEDFKSGKTKLQFVWGIVGG
                     SIDKNYMKPIQDGFKAMMNNGILAGYGIDSMKVRVFDGSMHAVDSKPIAFELCAKEGF
                     RAAAPKARPVLLEPIMKLEVVTPDEYVGPVIGDLNRRRGMPKGQEPRIGGAIVIQAEV
                     PLSEMFGYVTQLRTITSGRASSTMEFSHYAEAPAGIAKEVMEKAKGTAKV"
     gene            4590..4898
                     /gene="rpsJ"
                     /locus_tag="PK198_17585"
     CDS             4590..4898
                     /gene="rpsJ"
                     /locus_tag="PK198_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRF40611.1"
                     /translation="MNQKIRIKLRSYDHSLVDKSTEKIVKTVRNSGAVVTGPIPLPTE
                     KQVFTVLRSPHVNKKSREQFQLRTHKRVIEIYTPTQKTVDALSKLELPSGVDIQVKLT
                     "
     gene            5083..5709
                     /gene="rplC"
                     /locus_tag="PK198_17590"
     CDS             5083..5709
                     /gene="rplC"
                     /locus_tag="PK198_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRF40612.1"
                     /translation="MNGIIGKKVGMTSVYDGSGRNVACTVIEAGPCVVTQVKTQDSDG
                     YFALQLGYGDAKVKNTPKPMQGHFDKAKTGPKREVVEFRDFNAAPKSLGDLIKVDEVF
                     KEGDNVSAVGTSKGKGFQGVVKRHGFSGVGGQTHGQHNRGRAPGSIGACSYPAKVFKG
                     MRMGGRDGGSRVKIRNLKVVKIFPDQNLILVKGAIPGHNGSFVILEKK"
     gene            5720..6346
                     /gene="rplD"
                     /locus_tag="PK198_17595"
     CDS             5720..6346
                     /gene="rplD"
                     /locus_tag="PK198_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRF40613.1"
                     /translation="MKIEVLNIEGQQTGRSVELPDDIFGIEPNEHVVYLAVKQYLANQ
                     RQGTHKAKERSEMSGSTRKLHRQKGTGGSRKGDINNPLYHGGARVFGPRPRDYSQKLN
                     KKVKRLARKSALSSKAANGQIFVVEDFTFETPKTKSFKTMLGKLSVADRKTLLVTADH
                     DAALYLSGRNLQETAVVRAADLNTYEILKAQTLILAEGALDKIKQSLA"
     gene            6384..6680
                     /gene="rplW"
                     /locus_tag="PK198_17600"
     CDS             6384..6680
                     /gene="rplW"
                     /locus_tag="PK198_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRF40614.1"
                     /translation="MGKIVLVKPILTEKADALSEKLSQYTFIVDRKVNKVEIRKAVEA
                     MYNVAVDSVNTAVIPGKAKNRNTKKGVVRGQKAAYKKAIVTLAKGEEIDLFGNI"
     gene            6725..>7008
                     /gene="rplB"
                     /locus_tag="PK198_17605"
     CDS             6725..>7008
                     /gene="rplB"
                     /locus_tag="PK198_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659014.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRF40615.1"
                     /translation="MAVRKYNPVTPGTRFRVTNAFTEITTDKPEKSLLAPLKKSGGRN
                     SSGHLTIRQRGGGHKRRYRVIDFKRNKFDCKATVASIEYDPNRSARIALL"
BASE COUNT         1874 a         1787 c         1729 g         1555 t
ORIGIN      
        1 ctgagtcgcc ccttcactac ctccggaacc ccgaaaccca aacgcgaaat ttttccgacg
       61 ctgatcttta tgatttgtat gattaccgct gcccggagag gagtcacggg acgctccggt
      121 accggcgact tcagtcgaaa accaaccggc gactttagtc gctggtatca tcttcttcag
      181 cggtgagtgt gggcgggtag aacccgcagg atagtctgca caaggtagaa ccttgcgcag
      241 gcagggcgcg ggtagttttc cagcttagtg aaggggtgac tgccgggctn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnccccga aaccttgaac ccggaacctt gaacccgaaa
      361 cccgttccac accgaccttc tcactccctc aaaaactcca gttcctccgt ctcctccgac
      421 agccgcacca tcaccgatgg ctcgccggga ctgcgttccg actgacggtg ccgcaccaca
      481 tgattgcagc cttgtatgat ttcagaactg atgccgccga acgtggcagg gtagggagcg
      541 ccttccgtga cagcgggcgc cgataagacc gggccgccga aagcctgcag caatcctaca
      601 caaaatgaat cccgcacaat gcgcagcgct accgagccgt ccgggtgcaa cgcctcttcc
      661 ggcagatggt ctgctcccgg cgcgaccacc gtgagcggcc gctgatgaaa atagagcaaa
      721 gtctccaacc gtggatgcag atgtgccgtg agttgtttta acgcttccaa tgaccaaacc
      781 aacagctcgg tttgcgtggc atccggcagg cgtgtcaatt gcagcaacct gcgtatagct
      841 gcgggatgtn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg tgggaaacaa aacaagcccc
      901 ccgttttcca aagtacgcag agcgccgtcc aagtcggagg gataggttaa ccaggaagca
      961 gaggcgtgtg tagatgtcgt cataaaaaca agagtaagtt tgtgtagtcg tcctgtctgt
     1021 tttcaaggca cacaaacgcc gccgttttgc ggtttagtgc agggtacgcc atatattaat
     1081 agtattggct tctaaattgt agcagaataa gaggaatctg tttaatatcc gctaaaaatt
     1141 tttcattccc gaataagcat tgcctttcgt tttgcacttg cccaaattcc atactttgac
     1201 cggatataat tctgtgaggc cctattccga ccgaacgtgt tgagcgtttg aaaacggaat
     1261 tttactttta ttgaaaaaat atttttttag tgcttggtta tttgtctgga atgtatgtac
     1321 ctttgcactc gcttcgcaaa aaagctgttg ctgtaagcaa gcaaattcaa gaaaatcaac
     1381 gttttcaacc gtataagaac aaggacgtat gccaactatt aatcaattag tgcgtcacgg
     1441 cagggaaaaa gtggtcgtca agagcaaatc ccgcgctctg gatgcttgcc cgcaaaaacg
     1501 cggcgtatgc accaaagtat ataccactac gcccaagaag cccaactccg ctttgcgtaa
     1561 agtggccaag gtgcgcctca ccaatcagat tgaggtcatc gcctacattc ccggagaagg
     1621 ccacaacttg caggaacact ccatcgtact ggtagcaggc ggcagggtga aagaccttcc
     1681 cggtgtgcgt tacaccatcg tgcgtggctc ccttgataca cagggtgtca acaaccgcaa
     1741 gaagagccgt tcattgtacg gtaccaaacg tcctaaaaaa taatattagc cggcattaca
     1801 aagctttgct gaaatgagaa aaagaaaacc aaaagtaaga gtcattgcac aggatcctcg
     1861 ttacacggat cccatgatca cgcagtttgt caacaacttg atgtgggacg gtaaaaaaag
     1921 taccgccttc gaactcttct ataatgccat ggacttggtg gctcagcgca ccagcacaga
     1981 cgatgttcac gccgtctgga aaaaagcact taataacgta atgccgcaag tggaagtgcg
     2041 cagccgtcgc atcgggggcg ctactttcca gattccgacg gaaatccgcg ccaaacgcaa
     2101 agtttccatc ggtatgaaat ggctcatcaa attcgcccgc cagcgcagtg gtaaaggtat
     2161 ggcagatcgc cttgccgccg aaattgtcgc cgcaagcaaa ggggaaggcg ccgctgtaaa
     2221 acggaaagag gacatgcaca aaatggcaga agccaaccgt gctttcgctc acttcaaggt
     2281 ttaaagcaac gataaactaa aactgaaaat cattatctcc tgacagccat gagcaaggat
     2341 cttaaattca caagaaacat cggcattgcc gcccacattg acgctggtaa aactactacc
     2401 actgagcgtg ttttgtacta caccggtctt acccacaaaa tcggagaagt acatgagggc
     2461 gcagctacca tggactggat ggagcaagag caggagcgcg gtattaccat tacttctgcc
     2521 gctaccaaaa ctgcctggcg ctggaaagaa gaagaatata ccgtcaatat cattgacacc
     2581 cccggccacg ttgacttcac cgtggaagtg aaccgctctt tgcgcgttct cgatggcttg
     2641 gtgttcctct tctgtgctgt atcaggcgta gaacctcaat ctgaaaccaa ctggcgcctc
     2701 gccgataact acaaagtacc tcgcatcggt ttcgtcaaca agatggaccg ctccggcgcc
     2761 gatttcttca gcgttgttaa tgacgtgcgt accaaacttg gctccaacgc cattccgctt
     2821 caggtaccca ttggcgccga agaaaaattc cgtggcgtgg tggaccttat caccaacgag
     2881 gccatcatct ggaacgagca cgataaaggc atgacctacg aggtaattcc catcccggaa
     2941 gacctcaaag agaccgtcac cgaatggcgt accaaactcg ttgaagaagt agatattcca
     3001 gcttccgaaa aagagcgcgc catggaaatg cacaacgccc tcgtagaagc tgccgctgaa
     3061 aacgaggagg gcctgatgga aaaattcttt gaagatccga actccatctc agctgaagaa
     3121 atgcgtgcag ctatccgcgc tgccgttatg gacatgaagt ttgtgcccat gatgtgtggt
     3181 tcttccttca aaaataaagg tgttcaggct gtgttggatg cagtatgcgc cttcttgcct
     3241 tctcccaccg acgtggatgc catcaaaggc gtcaatccca aaacggatca ggaggaaatt
     3301 cgcaaaccat ctgtcactga gccgtttgcc gctttggcat tcaaaattgc taccgacccg
     3361 tatgtaggtc gcttggcttt catgcgcgtt tactctggcg ctttggatgc aggttcttat
     3421 gtttacaatt cccgttctga ttccaaagag cgtatttcgc gcctttatca gatgcacgcc
     3481 aataagcaaa atgcaattga tcgtgtagaa gcaggtgata ttgcagcagc cgtaggcttc
     3541 aaagacattc gcaccggcga tacgctgtgc gacctcgaca agcccatcgt gctggaaagc
     3601 atggtattcc ccgaaccggt gattgccatt gccgtagaac cgaagtctca gaaagaactc
     3661 gataagttgg gcatggcttt ggctaaattg gccgaagaag accccacctt ccgcgtaaaa
     3721 tatgacgaag acaccaacca gaccgttatc agcggtatgg gtgagttgca cctcgaaatc
     3781 attgtggatc gtctccgccg tgagttcaaa gtggaatgta accaaggtgc gcctcaggta
     3841 aactacaaag aagccctcac cggcacagtt tcccaccgcg aaaagctcaa aaagcagaca
     3901 ggtggttccg gtctcttcgc cgacatggaa tttgaactcg gccccgctga tgctgaattc
     3961 ctcgaaacgg aagacttcaa gagcggcaaa accaagctcc aatttgtatg gggtattgta
     4021 ggcggctcca ttgataagaa ctatatgaag ccgattcagg atggtttcaa agccatgatg
     4081 aacaacggca ttctcgccgg ctacggcatt gacagcatga aagtgcgcgt atttgacggc
     4141 tccatgcacg cagtggactc caaacctatc gcatttgaat tgtgcgctaa ggaaggtttc
     4201 cgtgcagcgg cccccaaagc tcgccccgta ttgctcgaac ccatcatgaa gttggaagtg
     4261 gttactcccg acgaatatgt cggccccgtt atcggcgacc tcaaccgtcg tcgcggtatg
     4321 cccaaaggcc aggagcctcg catcggcggc gccatcgtta tccaggctga agtgccgctt
     4381 tccgaaatgt tcggttatgt gacccaactt cgcaccatca cctctggtcg cgccagctct
     4441 actatggagt tctcgcacta tgcagaagct ccggcaggca tcgccaaaga agtaatggaa
     4501 aaagccaaag gcacagctaa agtttaattg aatttcattt tgtgggcgag ttcctgactt
     4561 tcccacaatt ttataaaacg tttttaggca tgaatcaaaa gatcagaatc aaacttcgtt
     4621 cgtatgacca cagcttagtg gacaaatcaa cggagaagat cgtgaaaacg gttcgcaaca
     4681 gcggcgctgt agtgacggga ccgattccgc tgcccaccga gaaacaagtg ttcacagtgc
     4741 tccgttctcc gcacgtcaac aaaaaatctc gggagcagtt ccagttgcgt acccacaagc
     4801 gtgttatcga aatttacacg cccacccaga aaacagtgga cgccctctcc aagttggaac
     4861 ttcccagcgg tgtggacatt caggtgaagt tgacgtgatc ttttcttttt cataagttgc
     4921 tgactcgcgc ctttcttttg gcgcacacaa ggcgagttta taaatgcttc caccaccgga
     4981 tttccgggtg tgatgtcgtt ggcagtactg ctctcggctg tgcataattg ctcattcttg
     5041 ggtgccaaaa caaggtatca ggcataataa aacattcaac cgatgaacgg aattatcgga
     5101 aaaaaagtcg gaatgaccag cgtctatgac ggctcaggcc gcaacgttgc ctgcacagta
     5161 attgaagcag gcccttgcgt agtcactcag gtcaaaacgc aagactctga cggctacttc
     5221 gccctgcaat tgggctatgg agacgccaaa gtaaaaaaca cgcccaagcc gatgcaaggg
     5281 cactttgaca aagccaaaac cggacctaag cgcgaagtgg tggaattccg cgacttcaac
     5341 gctgcaccga agtctttggg cgacttgatc aaagtggacg aggtattcaa agaaggcgac
     5401 aatgtgagcg ccgttggaac ctcaaaaggc aaaggttttc agggtgttgt gaaacgccac
     5461 ggctttagcg gtgttggcgg ccaaacacac ggtcagcaca accgcggtcg cgctccaggt
     5521 tctatcggcg cctgttctta tcctgcgaag gtattcaagg gtatgcgaat gggtggtcgt
     5581 gatggcggct cccgcgtgaa aatccgcaac ctcaaagtgg tgaagatttt ccctgatcag
     5641 aacctcattc tggtgaaagg cgccattccc ggccataacg gttcttttgt tattcttgaa
     5701 aaaaagtaaa taaacggcca tgaaaatcga agttctcaac attgaaggcc aacaaaccgg
     5761 cagatcggtg gaattgcctg acgacatctt cggcattgag ccgaatgagc atgtcgttta
     5821 cctggccgtt aagcaatatc tggccaacca gcgtcaaggt acgcacaaag ccaaagaacg
     5881 cagtgaaatg agcggttcta cccgcaaact tcaccgtcag aaaggcaccg gcggctctcg
     5941 taaaggggat atcaacaacc ccttgtatca tggcggcgcc cgtgttttcg gtcctcgtcc
     6001 gagagattac agccaaaagc tgaataaaaa agtgaagcgc ttagccagaa aatccgcgct
     6061 gtccagcaaa gctgccaacg gtcaaatctt cgtggtagaa gactttacct tcgaaactcc
     6121 gaagaccaag tctttcaaaa cgatgctggg caagctttcc gtagcagatc gcaaaactct
     6181 gctggtgact gccgatcacg atgctgcact gtatttgtct ggtcgcaact tgcaggaaac
     6241 tgctgtggtg cgtgccgccg acctgaatac ttatgagata ctcaaggctc agacgctgat
     6301 tctggcagaa ggcgctttgg ataaaatcaa acaatcattg gcttaattgc ggctgctagt
     6361 cagtttgctt taaatgaata gacatgggaa agatagtgct tgtgaagccc atcctcaccg
     6421 aaaaagcgga tgcgctttct gaaaagctca gccaatacac tttcatcgtt gatcgcaagg
     6481 tgaacaaagt tgagatcaga aaggcggtgg aggccatgta caacgtagct gttgactctg
     6541 taaacactgc ggtaattccg gggaaagcaa aaaaccggaa caccaaaaaa ggtgtagtcc
     6601 gaggccaaaa agcggcttat aaaaaagcca tcgttacctt ggccaaaggg gaagaaattg
     6661 atttgttcgg taatatttaa ttcgccggtt ttcggcgctg taaacgtatc aaaaactgtg
     6721 cgaaatggca gttagaaaat acaatccggt cactccggga acccgttttc gggtaaccaa
     6781 tgccttcacg gaaatcacga ctgataagcc ggagaagagc ctgctcgctc cgttgaagaa
     6841 gtcgggtggt agaaacagca gcggtcacct caccattcgt cagcgtggtg gtggtcacaa
     6901 gcggcgctat cgcgtgattg acttcaagcg taacaagttt gactgcaagg cgacggttgc
     6961 gtcgatcgag tacgatccga accgcagcgc ccgcatcgct ctgctcaa
//
LOCUS       DAORLF010000253         7001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5813_length_7001_cov_1.902102, whole genome shotgun sequence.
ACCESSION   DAORLF010000253 DAORLF010000000
VERSION     DAORLF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5813_length_7001_cov_1.902102"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK198_17610"
     CDS             complement(<1..847)
                     /locus_tag="PK198_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRF40616.1"
                     /translation="MTILIALAALGSLTLALAIMLMVANKKLYVYEDPRIDTVEDMLP
                     HANCGACGFPGCRPFAEALVTGKALPGKCTVSTDEGRQRIAQYLGVALGAEEKRVARL
                     ACGGGTNVARNRAQYHGISSCRAATLVSGGGKGCFWGCLGLGDCAVVCDFDAITMSAN
                     GLPVVDMNKCTACGDCVEACPKDLFSLQPVSHRLWVNCKNLEAGDDILEECRVACTAC
                     GKCAMDAPGDLITMIHNLPVVNYAENHRTQKPIERCPSGAIVWIDDDGTPIKGQESRK
                     IIRKGR"
     gene            complement(922..>1567)
                     /locus_tag="PK198_17615"
     CDS             complement(922..>1567)
                     /locus_tag="PK198_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40617.1"
                     /translation="VADLPAKSNGKQQLTMLVKRCSYVDEFSGEMHKGVASNYLCDRK
                     PGDRISITGPHRLPFVVPEDKTANLILIGMGTGIAPFRAFIKNIYKNVGDWQGKVRLF
                     YGARSGLELLYLNDQDGDLTNYYDEATFKAFHAISPRPHWSDPIALDAAIEARAAEII
                     EMLSQANTYIYVAGYEQILGMLNKAFANILGSKEKWETRKAELVAGNKWAEIIY"
     assembly_gap    1568..1669
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1938..2132)
                     /locus_tag="PK198_17620"
     CDS             complement(1938..2132)
                     /locus_tag="PK198_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40618.1"
                     /translation="MVKSLFTAIGILVGLVLAWIAVQALWRKVFADYVHDDDVLAERR
                     ECGNCGCTTACERKPVGSGQ"
     gene            complement(2595..3179)
                     /locus_tag="PK198_17625"
     CDS             complement(2595..3179)
                     /locus_tag="PK198_17625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HRF40619.1"
                     /translation="MNQESLFAIFINASLINNFVLAYFLGICPFLGVSGKMETATKMG
                     AAVTFVMIISSVCAYGLHALLVSIDAIYLQLISYIVVIAATVQLVEMFIKKMSPALFR
                     ALGIFLPLITTNCAILGLALFQTNKGYGLAQSVVYALGAGIGFTLALVLMAGLREKLN
                     FAETPNLVKGTALTLLIAGILSLAFMGFAGLGSN"
     gene            complement(3176..3832)
                     /locus_tag="PK198_17630"
     CDS             complement(3176..3832)
                     /locus_tag="PK198_17630"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRF40620.1"
                     /translation="MSQLTKDKTGEGFWGNLANSPSTNEFLKGLWKENPVFVQVLGMC
                     PTLAVTNTAMNGLAMGLATTFVLLMSNILVSSLRNVIPKQVRIASYILIIATFVTMTD
                     YAIQAISVDLSKALGAFISLIVVNCLILGRAEAFASKNTVGKSMLDALGMGFGFVIGL
                     LSLGAVREILGNGSIFGIPVFPASFQEWVIMILPPGGFFTLAMWLMFFNHRKQKKAAL
                     "
     gene            complement(3829..>4465)
                     /locus_tag="PK198_17635"
     CDS             complement(3829..>4465)
                     /locus_tag="PK198_17635"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRF40621.1"
                     /translation="LTMTGIGVFCALMIVLSYQGTLPRIEQLKAEALEKAIFKVVPGM
                     ESKRTFRMESDGSFTEVSGKEKDARLVYAGYDKEGKLVGVAVEAAGMGFADVIKVIYG
                     YDPEKEAVVGFYVLESKETPGLGDKIEKDPNFLANFEALDVSLTEDKSKTKNTVVAVK
                     HGEKKNNWEVDGITGATISSRAIGNLLGTSTAEMAPVLHKNKAFFEVKKGQ"
     assembly_gap    4466..4498
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4515..>4821)
                     /locus_tag="PK198_17640"
     CDS             complement(4515..>4821)
                     /locus_tag="PK198_17640"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRF40622.1"
                     /translation="SGALYLANKEAYPSPDFMLFSGGLMLGAVFMATDMVGSPVMPAA
                     VWLYGALIGVLVVVIRIWGGLPEGVMYAILLGNAVAPHLDNLIRPRVYGTVKTAKKS"
     assembly_gap    4822..4831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4832..5539)
                     /locus_tag="PK198_17645"
     CDS             complement(<4832..5539)
                     /locus_tag="PK198_17645"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRF40623.1"
                     /translation="MFKKTLHISTSPHLTKGIGTDDIMRNVVWAMLPVALYSVWSFGL
                     GALVVLATSTTACVLTEHFLAKISKKESTISDWSAAITGLLLGLTLPPGFPMWMAFLG
                     GVVSIALGKFVFGGLGYNVFNPALVGRAFLQAAFPVAITTWHPVYAPDRFTTVPSATF
                     TAPFMEPSTVDAFSGATPLSAFKFDHVVTNSYDLALGLIPGSTGETCAALILLGGLYL
                     IARNMMNWRIPASILLTV"
     gene            complement(5541..>6080)
                     /locus_tag="PK198_17650"
     CDS             complement(5541..>6080)
                     /locus_tag="PK198_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF40624.1"
                     /translation="NVATTAEIGRLLPHGLGIQERVVTITGPGVKKKGNYLIPIGTPL
                     RFVLEQVEADENISEVYMGGPMMGVAVSNLDISITKGTSGIVVFTEEQVKRPDKVYPC
                     INCGACVDACPISLTPNKLGILAKFEAYDEMAEQYNLMDCFECGSCSYVCPSHIPLVQ
                     YFRLAKTTVRKRKAAKPSA"
     assembly_gap    6081..6090
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6091..>6755)
                     /locus_tag="PK198_17655"
     CDS             complement(<6091..>6755)
                     /locus_tag="PK198_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HRF40625.1"
                     /translation="PSQPVVREGQEVHRGQLLAKAGGYMSVPLHAPVSGVVRKIGNVP
                     AISGKMGQGIYLEAFHYSTQEIAEGTPIDLDTATPAEILQGIQDAGIVGLGGAAFPTH
                     VKLKIPEGKHCDALIINGIECEPYLTTDHRVMLEQAHDIFTGIRYLLKATGADKCVIG
                     IEANKQDAADHLAGLIPSDLPVTVKVVPVKYPQGAEKMLVTAILGREVPSGGLPIDIG
                     AVV"
     assembly_gap    6756..6768
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1519 a         1957 c         1784 g         1573 t
ORIGIN      
        1 gtctgccctt ccgaatgatc ttcctgctct cctgtccttt gatgggcgta ccgtcgtcgt
       61 cgatccagac gatggcgccg ctggggcatc gctcgatggg cttttgtgtg cggtggtttt
      121 cagcataatt gaccaccggc agattgtgta tcatcgtgat gaggtcgccg ggcgcatcca
      181 tggcgcattt gccgcaggcc gtacaagcca cccggcactc ttccagaata tcgtcgccgg
      241 cctccaagtt tttgcagttc acccacagcc tgtggctcac gggttgcagc gaaaacaagt
      301 ctttcggaca agcctccacg cagtcgccgc aggccgtgca tttgttcata tccaccaccg
      361 gcaacccgtt ggcgctcatg gtaatggcat cgaaatcgca gaccaccgca caatcgccca
      421 gtccgaggca accccagaaa cagcccttgc cgccgccgga taccaacgta gccgcccggc
      481 aggaggaaat gccgtgatat tgcgcgcgat tgcgggccac attcgtgccg ccgccgcaag
      541 ccagccgggc tacccgcttt tcttcagcgc ccaacgctac gccgaggtat tgggcaatgc
      601 gttgccgccc ctcgtcggtg ctcacggtac acttgccggg cagcgccttg ccggtcacca
      661 aagcctccgc aaaggggcgg caaccgggaa aaccgcaggc cccgcagttg gcatgtggca
      721 gcatgtcttc caccgtgtct atgcggggat cttcatagac gtagagcttc ttgttggcta
      781 ccatgagcat gatggccaat gccagcgtga ggctgcccaa tgcggcaagt gcgatgagga
      841 tggtcatggt ttgttgtctt gtagtttcgg cggttagctg ccggcttgta gctaatagcc
      901 aacagctaat agccgttccc atcagtaaat aatctccgcc cacttattcc cggccaccaa
      961 ttccgctttg cgggtttccc acttttcttt ggaacccagg atattggcga aagccttgtt
     1021 cagcatacca aggatttgct cgtagccggc cacataaatg taggtattgg cttgcgagag
     1081 catctcgatg atttcggcgg cgcgcgcttc aatggcggca tccaaggcga tggggtccga
     1141 ccagtgcggg cgcggactga tggcgtggaa agccttgaag gtagcctcgt cgtagtagtt
     1201 ggtcaggtcg ccgtcctgat cgttgaggta cagcaattcc agaccgctgc gagcgccgta
     1261 gaacagccgg actttgccct gccagtcgcc cacattttta taaatatttt tgataaaagc
     1321 cctgaacgga gcaatgccgg tgcccatgcc gatgaggatg aggttggcag ttttgtcttc
     1381 gggtacgaca aaaggcaggc ggtgcggccc ggtgatggag atgcggtcgc cgggtttgcg
     1441 gtcgcacaag tagttggaag ctacgccttt gtgcatttcg ccactgaatt cgtccacata
     1501 cgagcagcgc ttgaccaaca tggtgagctg ctgtttgccg ttggacttcg caggcagatc
     1561 ggccacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagcaacagt
     1681 tcgcgcactt cctcagtatc gggaggcgtc aggcgcacag attggaggat cgttgcctga
     1741 aaggtgttgt cggtattaaa atccgctaaa cgtgccatgg tttatgtatt ggttatgaat
     1801 gttttacttt ttttgtccgc tgcaagcctc aagcctcaag ctgcaagcct caagctgctg
     1861 gctgttggct acaagctact ggctgtaagc cttaagctac aagtctcaag ctatggtcac
     1921 tgtacgcttc gcaccgccta ctgcccactg cctaccggct tcctctcaca cgccgtcgta
     1981 cacccgcaat tcccgcactc tctgcgctcg gccagcacat cgtcatcatg cacataatcg
     2041 gcaaaaacct tgcgccacaa agcctgcacc gctatccagg cgagcacaag ccccaccaga
     2101 atcccaattg ccgtaaaaag tgatttgacc atttttttta tctgttttat ccaacttagg
     2161 aacggttcga aaataatagt tccaatttag tgtggctata gaaaccttat ttttgaatcc
     2221 ttgctacgtt tacgaatcct gtaaggcttc gcaaacgttt tgccggcggt tgcgaagcct
     2281 ctgaggattc gtaaccgtaa aattaatttt gatacctcac ccactcaccc ctcaccccca
     2341 gcgcaaacgc atttaaacgg caaaatataa aaccgcaaaa tgtaaaaccg caaagtgcgt
     2401 agcgtactct ccatgaggga gcagtctcgc ctgcttttag ggcgcctctg cgggtgattt
     2461 ttacatttta catttgatat tttacggttt tgcggttttc aatttttcac tcttttgaaa
     2521 ttttatttta aatgcgtttt ctctggccac tcacacctca ctcactcacc cacccactca
     2581 ctcacccctc acccctaatt actccccagc cccgcaaacc ccataaacgc cagcgacaaa
     2641 atgcccgcaa tgagcagcgt cagcgccgtg cctttcacca aattgggcgt ttccgcaaaa
     2701 ttcaacttct cgcgcaaacc cgccatcagc accaatgcca gcgtgaaacc aatgcccgcg
     2761 ccgagtgcat acaccacgct ttgcgccaag ccgtagcctt tgttggtttg aaacagggcc
     2821 agaccgagaa tcgcgcagtt ggtcgtgatg agcggcagga agatacccag tgcccgaaac
     2881 agcgccggac tcattttttt gatgaacatc tctaccaatt gtaccgtagc agcaatgacg
     2941 acgatatagc tgatgagttg gagataaatc gcgtcaatgg agacgagcag cgcgtgcagt
     3001 ccataagcgc ataccgagct aataatcatt acgaaagtaa cggcggcgcc cattttggtg
     3061 gccgtttcca tcttgccgga tacgcccaga aacgggcaga tgcccaagaa gtacgccaac
     3121 acgaaattgt tgatcaagct ggcattgatg aagatggcaa atagcgattc ctgattcata
     3181 atgcagcctt tttttgcttt ctgtgattaa aaaacatcag ccacatagcg agggtgaaaa
     3241 agccacccgg cggcaatatc ataatgaccc actcctgaaa acttgcagga aatacaggga
     3301 tgccgaagat gctgccgttg cccaatattt cgcgcacagc gcccagcgac aacaacccga
     3361 tgacgaagcc gaaacccatg cccaacgcat cgagcatgga cttgccgact gtatttttgg
     3421 atgcaaaggc ttcggccctg cccagaatca ggcagttgac cacgatgagc gaaatgaatg
     3481 cacccaatgc tttcgacaaa tccacgctga tggcctgtat ggcgtagtcg gtcatggtga
     3541 caaaggtggc aatgatgagg atgtaggaag ctatacggac ttgcttgggt atgacattgc
     3601 gcaacgacga aaccaaaata ttcgacatga gcagcacgaa ggtagtggcc aagcccattg
     3661 ccagcccgtt catggcggtg ttcgtaacgg ccagcgtggg gcacataccg agcacctgca
     3721 caaatacggg attctccttc cacaagccct tgaggaactc gttggtggaa gggctgttgg
     3781 ccaaattgcc ccagaacccc tcgccggtct tgtctttcgt cagttggctc attgtccttt
     3841 ttttacctca aaaaatgctt tgtttttatg caataccggc gccatttcgg cggtgcttgt
     3901 tcccaaaagg ttgccgatgg ccctcgaaga aatggtggcg ccggtgatgc cgtccacttc
     3961 ccagttgttt ttcttttcgc catgttttac tgcgacaacc gtgtttttcg ttttgctttt
     4021 atcttctgtt aaagacacgt ccaatgcctc gaaattggcc aaaaagttcg ggtctttttc
     4081 aatcttatct cccaagccgg gggtctcttt gctttccaaa acataaaatc ccaccactgc
     4141 ctctttttca ggatcgtacc cgtaaatcac cttgatgacg tcggcaaatc ccataccggc
     4201 agcctctacg gccacgccca ccaatttgcc ctccttgtcg taaccggcat acactaagcg
     4261 ggcgtctttt tctttgccgc tcacttccgt gaaactgccg tcggactcca tgcggaaggt
     4321 ccgtttcgac tccatgcccg gcaccacttt aaaaatggct ttttccaatg cctcggcctt
     4381 cagttgttct atgcgcggca atgtgccctg gtaggaaagc acgatcatca gggcgcaaaa
     4441 cacgccgatg cccgtcatgg tcagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     4501 attgcccgct tctttcatga ctttttcgct gtttttaccg tgccatacac cctgggcctg
     4561 atgaggttgt ccaaatgcgg ggccacggca ttgcccaata aaatggcgta catcacccct
     4621 tccggcaggc cgccccagat gcggatgacc accaccaaca cgccgatgag cgcgccgtag
     4681 agccacacgg ctgccggcat cacgggcgat cccaccatat cggtagccat gaacaccgcg
     4741 cccagcatga gtccgcccga aaagagcata aaatcagggc ttggataggc ttctttgttg
     4801 gccaaataca aggccccgct cnnnnnnnnn ntaccgtgag gagaatggat gccggaatgc
     4861 gccagttcat catgttgcgg gctatcaggt acaggccgcc caagagaatg agcgccgcgc
     4921 aggtctcgcc ggtcgagccg ggaatgaggc ccaatgccag gtcgtagctg ttggtaacaa
     4981 cgtggtcaaa tttgaaggca gacaaaggcg tagcaccgct gaaggcgtcc acggtactcg
     5041 gctccataaa cggcgctgtg aacgtggcgg aaggcactgt cgtgaagcgg tcgggggcgt
     5101 acacggggtg ccaggtagta atggccaccg gaaatgccgc ctgaaggaaa gcgcgcccca
     5161 ccaatgcggg gttgaacacg ttgtacccca agccgccaaa gacaaacttg cccaaagcga
     5221 tggacaccac gccgcccaaa aacgccatcc acatcgggaa gccgggcggc agcgtcagtc
     5281 ccagcagcaa gccggtgatg gccgccgacc aatcggaaat ggtactttct tttttggata
     5341 ttttggctaa aaaatgctct gtgagcacac aggctgtcgt gctcgtggcc aataccacca
     5401 aggcgcccaa gccaaacgac cacaccgaat acaaggctac cggcagcatg gcccaaacca
     5461 cgttgcgcat gatgtcgtcg gtgccgatgc ctttggtcag gtgcggcgag gtgctgatat
     5521 gtagcgtttt tttgaacatg ttacgcactc ggtttggcag ctttgcgctt tctcacggtc
     5581 gttttggcca atctgaaata ttgcaccaac ggtatgtgag aaggacaaac ataagaacag
     5641 gaaccacact cgaagcagtc catcagattg tactgctcgg ccatttcgtc gtaggcttca
     5701 aacttggcca atatacccaa tttgttgggc gtcagtgaaa tagggcaagc atccacgcag
     5761 gcgccgcagt tgatgcaggg atacaccttg tcggggcgtt tgacctgctc ttccgtaaaa
     5821 acgacgatgc cggaggtgcc tttggtaatc gaaatatcga ggttggacac ggccacgccc
     5881 atcatggggc cgcccatata tacctcgctg atgttttcgt cggcctccac ctgttccagc
     5941 acaaaccgca gcggcgtgcc gatgggaatg aggtagttgc ctttcttttt cacgcccggc
     6001 ccggtgatgg tgaccacgcg ctcctgaatg cccaatccgt gcggaagcaa tcttccgatt
     6061 tcggcggtgg tggccacatt nnnnnnnnnn gacgaccgcg ccgatgtcta tgggcaatcc
     6121 gccggaaggc acttcgcggc ccaaaatggc ggtgacgagc atcttttctg ccccctgtgg
     6181 gtacttcacg ggcaccacct ttaccgtcac gggcaggtcg gaaggaatca gcccggcgag
     6241 gtggtcggcg gcgtcttgct tgttggcttc tatgccgatg acgcacttgt cggcgccggt
     6301 ggctttaagc aaataccgga tgccggtgaa aatatcatgg gcctgctcca gcatcacgcg
     6361 gtggtcggtg gtaagatacg gctcgcactc tataccattg ataatgagcg catcacagtg
     6421 tttgccttcg ggtattttta atttgacgtg ggtgggaaaa gccgcgccgc ccaatcctac
     6481 aatccccgca tcctgaatgc cctgaagaat ctctgccggc gtggctgtgt cgagatcaat
     6541 gggcgtgccc tctgcaattt cctgcgtaga ataatggaag gcttccagat aaatgccctg
     6601 ccccatcttg cccgaaatgg ccggcacatt gcctattttg cgcaccactc ccgacaccgg
     6661 cgcgtgcagc ggcaccgaca tgtagccgcc ggccttggcc agcaactgcc ctctgtgtac
     6721 ctcctgcccc tcgcgcacga cgggctgcga aggcgnnnnn nnnnnnnntg ctgcgacatc
     6781 ggcagaatca acacgggcgc aaaggagaac tgacggacgg gcagcccttt ggtctcatcc
     6841 ttgtgctccg gagggtgtac cccgtgcttg aaggtgttgt gccggaaatt gaataatttc
     6901 atcgtcggtt tgctttcagt gtgctgtgta taatgctcgg tgcgcagtgc ggtgtatgtg
     6961 gtgtgtgccg tcgcgcttac cgcgccacgg cccgcgcaag g
//
LOCUS       DAORLF010000254         6907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5907_length_6907_cov_1.829393, whole genome shotgun sequence.
ACCESSION   DAORLF010000254 DAORLF010000000
VERSION     DAORLF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5907_length_6907_cov_1.829393"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>234
                     /locus_tag="PK198_17660"
     CDS             42..>234
                     /locus_tag="PK198_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40626.1"
                     /translation="MRQQIFTALAILGAIFMQAIANAQSYTPITGQQGIAMFYADYFE
                     GQTTAHGEVYRKTELTCSHG"
     assembly_gap    235..376
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <377..815
                     /locus_tag="PK198_17665"
     CDS             <377..815
                     /locus_tag="PK198_17665"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF40627.1"
                     /translation="IINAGKARVTVEQTGYSDTNPVSAFRARSASSYASASYSSEFTA
                     KGGAAPVAAAGYYGPSPSGAFGGYGLQLGAYSLYDNALRRQEGLLNQGVAGVLIRENM
                     TAEGGRMYRVVIGSFASRTDAETYLRDYLKPNYIADGVVVQLK"
     gene            824..1450
                     /locus_tag="PK198_17670"
     CDS             824..1450
                     /locus_tag="PK198_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40628.1"
                     /translation="MNKHFFFLLFIGLLGLTSCNNAAQQETSKDQTAVQASAEPAGDY
                     SKYELTQLPGTSMQKAVLRNQAGRVIEQGYLDDKRLKTGEWLSFYENSGLPYKLINYR
                     AGVQNGLYIFFNEIGQVSLLATYADNQLDGYWVKYRFGRLDEEAEYLKGQMHGTHRQY
                     FVREGWLQFTTEYKNGVRHGMSRTYNKEGQVTLEYEFKDGEKISGGMK"
     gene            complement(1501..>2235)
                     /locus_tag="PK198_17675"
     CDS             complement(1501..>2235)
                     /locus_tag="PK198_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRF40629.1"
                     /translation="DKIIKHSNGIMIARGDLGIEMPIEKLPVIQKDLIERCIQRARPV
                     IVATQMMDSMISNPSPTRAEATDVANAVLDGADAVMLSAETASGKFPVRVVEYMERII
                     REAEKHDMPQRKRPIPHVKKRTFLSDVICFNAARSAEEVNAKAIVGMTASGYTAFMVS
                     SYRPNKQIFIFSNRTQMLSTLNLVWGVRCFYYDRFTTTDETFQDVNEILKREGKVEPG
                     DVVINTGSMPIQERRRTNMMKISVVE"
     assembly_gap    2236..2294
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2295..2932)
                     /locus_tag="PK198_17680"
     CDS             complement(<2295..2932)
                     /locus_tag="PK198_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRF40630.1"
                     /translation="MRNVEHRSTKIVATVGPASNSPEMLLALVQAGVDTFRLNFSHGD
                     HQGHEQTINRICDINEKLGTHIGILADLQGPKLRVGKMAGEGLDLKEDDVVTFVNEPC
                     EGTMERIYMSYAQFPKDVNPGEMVLVDDGKLVFEVVDTNREDTVRLKVLHGGRLLSNK
                     GVNLPNTKVSLPALTAKDIADLDFILTQPVNWIALSFVRSPRDLKDLETRIEA"
     gene            complement(3041..3496)
                     /locus_tag="PK198_17685"
     CDS             complement(3041..3496)
                     /locus_tag="PK198_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF40631.1"
                     /translation="MQIFTDTPDLYISHLPEDRIAPMEALREVIKSNLPPGFEETMSY
                     GMIGYVVPHTLYPAGYHCDPKLPLPFMNIASQKNFVAVYHMGIYTLPELMDWFVTEYP
                     KHAKGKLDMGKSCIRFKKPDQIPFQLIGELASKMTVEDWVRVYESGLKR"
     gene            complement(3529..3900)
                     /locus_tag="PK198_17690"
     CDS             complement(3529..3900)
                     /locus_tag="PK198_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF40632.1"
                     /translation="MQLGVFSISLNVKDIRASKAFYEQLGFAEFGGNLDQNWLIMKNS
                     STLIGLFQGMFEGNLLTFNPGWDENAQNVDPFDDVRTIQRHLKSQGVALVTEADESSA
                     GPAYITLTDPDGNSILIDQHR"
     gene            4120..>5078
                     /locus_tag="PK198_17695"
     CDS             4120..>5078
                     /locus_tag="PK198_17695"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40633.1"
                     /translation="MPVHYIAWWNVENLFDTEISADRPDWLQRKLAAELKGWNEAALG
                     RKVSQLASVISSMNNGKGPDILGVCEVENMTVLQKLVDALAPLQRNYRIMFHAGPDQR
                     GIDVAFILDQDKYEIEDLTFSYEVLKRTGTRDIFQINIKTAKGHELILIANHWPARMG
                     GQYASEPYRMMCGETLSYWMSRIQEIRGANIPVLLMGDFEPFNRSLTEYANSSPTRAK
                     VNKGKIPYLYNLTQRLQSDSLRGSYVFDNEPMLIDQMLVSKGISLKGGPFDLDKSTLR
                     VYSRKDMISGLYDAPVRHSRPSEKSSYNPDGYSDHLPLVLELF"
     assembly_gap    5079..5113
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5114..>5416
                     /locus_tag="PK198_17700"
     CDS             <5114..>5416
                     /locus_tag="PK198_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40634.1"
                     /translation="QKGERIQSLALNAVNLEGLADALALLPELNALAISNRELPELPP
                     TITHCQKLGQLFISSAGLVRLPENLGDCRGLHSITLLHNKLRRLPASLSECLNLRA"
     assembly_gap    5417..5468
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5469..>5819
                     /locus_tag="PK198_17705"
     CDS             <5469..>5819
                     /locus_tag="PK198_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRF40635.1"
                     /translation="LPWIRTISFKNNRLISVPDVPWTCRMLADLDLSNNWIAVLPGNV
                     SLSNLEILRLRCNQLTELPECWLWSPKLKILDIARNPIRSLPALPNTLQHLDISGCPI
                     AGLPDQFFELTELR"
     assembly_gap    5820..5951
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5952..6613
                     /locus_tag="PK198_17710"
     CDS             <5952..6613
                     /locus_tag="PK198_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40636.1"
                     /translation="QCLSALSLALPNLRIAVLKHLKAGNALTGHPVPGAKMVLIGKFS
                     EPLKVLKSRLEGVGIELVAAPKADWVLLGRPPYRLPEQLPPVFSILTEDDLSSLKSEA
                     SAPPKDALQTARLRELLLHSDETNVELALLMLKNLRVSGLHTELLCARTRCRTPRLKR
                     RLSEQLRAITPLEELHVLKLPFPAWMKKPPPQAAAQMERQLAGTSFNGALLFQWLSAP
                     R"
     gene            6660..>6907
                     /locus_tag="PK198_17715"
     CDS             6660..>6907
                     /locus_tag="PK198_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40637.1"
                     /translation="MDYQLKHVLITGANEGIGKATAMALAKKGFAVIMACRNMEKAEA
                     ALAEIKNGSGNSNIELLPLDLSDLNSVQKAARLYRERYT"
BASE COUNT         1542 a         1709 c         1676 g         1560 t
ORIGIN      
        1 gaaatccgaa atcaatccct atctttgcgg caaaaacaat tatgagacaa caaattttca
       61 cagctttggc gattctgggc gctattttca tgcaggccat agccaatgca caatcttaca
      121 cgcccatcac ggggcagcag ggcattgcca tgttttatgc cgattatttc gagggacaaa
      181 caaccgcaca cggcgaagta tatcgaaaaa cggaactcac ctgctcgcac ggttnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnncatc atcaacgccg gtaaggcccg cgtaacggtg gagcagaccg
      421 gctattccga caccaatccg gtctcggcgt tcagagcgcg cagcgcctct tcttatgcct
      481 ccgcatcata cagctcggag tttactgcca aaggtggtgc agcgccggtt gcggcagcag
      541 gttattacgg cccttcgccg agtggcgctt tcgggggcta tggcctgcaa ttgggggctt
      601 actctttgta tgacaatgcg cttcgcaggc aggaaggtct tctcaatcaa ggagttgcag
      661 gtgtactcat cagggagaat atgacggcag agggggggcg catgtaccgg gtggtcatcg
      721 gctcattcgc ctcgcgcacc gatgcagaaa cttatctgcg cgattatttg aaacctaact
      781 acattgccga cggcgtggtg gtacaattga aataaccaat aacatgaaca agcatttctt
      841 ttttctcctc ttcatagggc tgctgggatt gacttcctgc aataatgccg ctcaacagga
      901 aacctccaaa gatcaaacgg cggtgcaagc atctgctgaa cctgccggcg attacagcaa
      961 gtacgaactg acgcaattgc ccggcacttc gatgcaaaaa gcagtgctgc gcaatcaggc
     1021 aggcagagtt attgagcagg gttacttgga cgacaagagg cttaaaacgg gcgagtggct
     1081 gtctttttat gaaaactcag gtcttccgta taaactcatc aactacaggg ccggcgttca
     1141 aaacggcttg tacatctttt ttaatgagat aggtcaggtt tcactattgg ccacttatgc
     1201 cgacaaccaa cttgacgggt attgggtaaa atatcgcttt ggtcgcttag atgaggaagc
     1261 agagtatctc aaggggcaaa tgcacggcac gcaccggcag tattttgtgc gagaaggctg
     1321 gttgcagttt accaccgagt acaaaaacgg agtacgccac ggtatgagcc gcacttacaa
     1381 caaagaaggc caggtgacgc tggaatacga gttcaaagac ggcgaaaaaa taagcggcgg
     1441 catgaaataa tgctccttag tagtggggca aaaatgacca ctttattttt ttatcgcccc
     1501 ttactctacc accgaaattt tcatcatgtt ggtgcgccgg cgctcctgaa tcggcatact
     1561 tcccgtgttg atgactacat ctccgggttc tactttacct tcccgtttca gaatttcatt
     1621 tacatcctga aaggtctcgt ctgttgtagt aaaccggtcg taataaaagc aacgaacgcc
     1681 ccaaaccaga ttcagtgtgg aaagcatctg cgtgcgatta gagaagataa atatctgttt
     1741 attaggccga tagcttgata ccatgaaggc cgtatagccc gaagcggtca tacctacaat
     1801 ggctttggca ttcacctcct ctgcagaacg ggctgcgttg aaacagatca catcagagag
     1861 gaaagtacgc ttttttacat gcggaatggg gcgcttgcgc tgtggcatat cgtgtttttc
     1921 cgcttcgcgg atgatgcgtt ccatgtattc caccacgcgc accggaaact tgcccgaagc
     1981 ggtttctgca ctcaacatca ccgcatcggc gccgtccaaa actgcattgg ccacgtcggt
     2041 agcttctgcg cgggtagggc tgggattgct gatcatactg tccatcatct gggtagccac
     2101 aatgacgggg cgggcgcgtt ggatgcacct ttcgatgagg tctttctgaa tgaccggcaa
     2161 tttttcgatg ggcatctcaa tgccgaggtc gccacgggca atcatgatgc cgttggagtg
     2221 ctttatgatt ttgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnngcctca atgcgcgttt cgaggtcttt aaggtcgcgg ggcgaacgca
     2341 cgaaggagag cgcaatccaa ttgacgggct gcgtgagaat gaaatctagg tcggcaatgt
     2401 ctttggcggt caaagccggc agcgacactt tggtgttcgg caggttgacg cctttgttcg
     2461 agagcaggcg gcccccatgg agcactttga gccgcacggt atcctccctg ttggtgtcca
     2521 ctacctcaaa gacgagctta ccgtcgtcca ccagcaccat ttcgcctgga ttgacatcct
     2581 taggaaattg cgcatagctc atataaatgc gctccatggt gccctcgcaa ggttcgttca
     2641 cgaaagtgac cacatcgtct tctttcagat caagcccctc gccggccatt ttgcctacgc
     2701 ggagtttggg gccttgcaag tcggccagaa tgccgatgtg ggtacccaat ttttcgttga
     2761 tatcgcatat ccggttgatg gtctgctcat ggccttggtg gtcgccatga gaaaagttga
     2821 gccggaaggt atctacgccg gcctggacta aagccagaag catttcgggc gaattggaag
     2881 ccgggcctac ggtggctacg attttggtgc tgcggtgttc tacgttgcgc atggatgttc
     2941 aatttgggcg gcggcaatcg ggccgctaag tgtacttttt acaaaaacat ggcaaaagta
     3001 caattaattt gaaaggggca acataaataa tcgcagcgca ctatctcttc aagccgcttt
     3061 catacactcg cacccagtct tccaccgtca ttttggaggc caattcgccg atgagctgga
     3121 acggaatctg gtcgggcttt ttgaagcgga tacagctttt gcccatgtcc aacttgcctt
     3181 tagcgtgctt gggatattcc gtaacgaacc aatccataag ttccgggagg gtgtagatgc
     3241 ccatgtgata gaccgccaca aagttttttt gggaagcaat gttcataaac ggcagaggga
     3301 gtttcggatc gcagtgatag ccggcgggat agagcgtgtg cggcaccaca tagccgatca
     3361 tgccgtagct catggtttcc tcaaaaccgg gcggcaggtt acttttgata acctcgcgca
     3421 gggcttccat gggagcaatg cggtcttcgg gcaggtgaga aatgtagagg tcgggagtgt
     3481 cggtgaagat ttgcatggcg tatgattttg ggtgtgaaga tcgctgtttt atctgtgctg
     3541 gtcaattaaa atgctgttgc cgtcggggtc ggtgagggtg atgtaagccg ggccggcact
     3601 gctttcgtcg gcttctgtga cgagcgccac tccctgcgat ttgagatgcc gttgaatagt
     3661 gcgcacatcg tcgaaggggt ccacattttg ggcgttctca tcccagccgg gattgaaggt
     3721 gaggaggttg ccctcaaaca tgccctgaaa cagcccgatg agggtgctgc tgtttttcat
     3781 aatcaaccag ttttgatcga ggttgccgcc gaactctgca aatccgagct gctcgtaaaa
     3841 tgccttcgac gcgcgaatgt ctttcacgtt gaggctgatg gaaaagacgc ctaattgcat
     3901 gtgttgagta ttttagatgt gaatgaagta acgtgagctt gatgaaacca aaaattcttc
     3961 tcccgttgga gagctgccga tgatctcccc ggcaaaaata atatttccaa agcattaaaa
     4021 cacaaaaagt tttaaaaaat tacataaatc tatttttgcc ctgctactca ctgtatttgc
     4081 tcgtaacttt tacatttgtc ttttcaaaac ttacaaccta tgcctgtaca ttacatcgcc
     4141 tggtggaacg tggaaaacct gtttgacacc gaaatcagcg ccgaccggcc cgattggctg
     4201 caacgcaaat tggccgccga actcaaaggc tggaacgaag cggctctggg gcgcaaagtc
     4261 tcccaattgg cttccgtcat ttcttccatg aacaacggca aaggccccga tattctcgga
     4321 gtgtgcgagg tggagaacat gaccgtgctg caaaaattgg tggatgccct ggcgccgctg
     4381 caacgcaact accgcatcat gtttcacgcc ggccccgacc aacgcggcat tgatgtagct
     4441 tttattctcg atcaggataa atacgaaatc gaagacctca ccttcagcta cgaggtgctc
     4501 aaacggaccg gtacccgcga catttttcaa attaacatca aaaccgctaa agggcacgag
     4561 ttgatcctca tcgccaacca ctggccggct cgtatgggcg ggcagtacgc ctccgagcct
     4621 taccgcatga tgtgcggcga aaccctcagc tactggatgt cgcgtattca ggagattcgc
     4681 ggcgccaata ttcccgtgtt gctgatggga gacttcgagc ctttcaaccg ctcgctcacc
     4741 gagtatgcca acagcagccc tacccgcgct aaagtcaata aaggcaagat accttatttg
     4801 tacaatctca ctcaacggct gcaaagcgac agcctcagag gctcctatgt gtttgacaat
     4861 gagccgatgc tgatagacca aatgttggta agtaaaggta tttccctcaa aggcggcccc
     4921 tttgatcttg acaaatccac gctcagggtt tattctcgca aggacatgat cagcgggttg
     4981 tatgatgctc ccgttcgcca cagccgccct tccgaaaaat cgtcttacaa tcccgacggt
     5041 tacagcgatc acctgcccct ggtgctggag ttgtttgann nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnacaaaaa ggggagcgga tacaaagcct tgcactgaac gctgtgaatt
     5161 tggaaggact ggcggatgct ttggcactgc tgccggaact aaacgctctg gccatttcca
     5221 accgggagct accggaactg ccgccgacca tcactcattg tcaaaagctt ggccaattgt
     5281 tcatcagtag tgccggcctc gttcgcctcc ccgaaaatct gggcgactgc cgggggctgc
     5341 actccattac cttactgcac aacaagcttc ggcggctgcc cgcatcactt tcggagtgtc
     5401 tgaatttgcg cgctttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnag ttgccctgga tcagaacgat cagttttaaa aacaaccgcc tgatctccgt
     5521 gcccgatgtg ccctggacct gccgaatgct cgccgactta gatttgagca acaactggat
     5581 cgccgtactg cccggaaatg tctccctgtc caatctggaa atcttgcgcc tgcgttgcaa
     5641 tcaactgacc gaactgccgg agtgctggct gtggtctccc aaactgaaaa tattagacat
     5701 tgcccgcaac cccattcgct ccctgcccgc actccccaac accctgcaac atttggacat
     5761 cagcggctgc ccgattgccg gtctccccga tcaattcttt gaactcaccg aattgcgcan
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nagcaatgtt tgtctgcgtt gtctctggct ttgcccaatc ttcggatcgc
     6001 tgttttgaaa cacctcaagg caggtaatgc tctgacaggc catcctgtgc cgggcgcaaa
     6061 aatggttttg atcggtaagt tttcggaacc gctgaaagtg ctgaaaagcc ggttggaagg
     6121 ggtcggaata gaactcgttg ctgcgccaaa agccgattgg gtgctgttag gcaggccgcc
     6181 gtaccgcttg cctgagcagt tgccgcccgt tttttccatc ctcacggaag acgacttatc
     6241 atctctcaaa agcgaagctt ctgcgcctcc gaaggacgcg ttgcaaactg cccgcctgcg
     6301 cgagttactc ctgcattccg acgagacgaa tgtagaactg gccttgctga tgttgaaaaa
     6361 cctgcgcgtt tcggggttgc acacagagtt gctttgcgcc agaacccgct gccgtacacc
     6421 ccgcctcaaa cgccggctgt cggagcaatt gcgtgccatc acgccgctgg aggaactgca
     6481 tgttttgaaa ctgccgttcc cagcatggat gaaaaaaccg ccgccacagg ccgctgcaca
     6541 gatggagcgc caattggcag gcacttcctt caacggcgcg ctgttgtttc aatggctttc
     6601 cgcgccgcgt tgaacaatct tgcttttcag gctttctatt cccgtcaaaa ccaacgaaaa
     6661 tggattacca gctcaaacac gttctcatca ccggcgccaa cgaaggcatc ggcaaggcta
     6721 cggcgatggc actggcaaaa aaaggctttg ccgtcatcat ggcttgtcgc aatatggaaa
     6781 aagcggaggc cgctctggcg gaaatcaaaa acggcagcgg caactccaat atagaactgc
     6841 tcccgctcga tctctccgac ctcaactcgg tgcaaaaggc cgcccggctc taccgcgagc
     6901 gctatac
//
LOCUS       DAORLF010000255         6889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5931_length_6889_cov_2.233099, whole genome shotgun sequence.
ACCESSION   DAORLF010000255 DAORLF010000000
VERSION     DAORLF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_6889_cov_2.233099"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>1243
                     /locus_tag="PK198_17720"
     CDS             8..>1243
                     /locus_tag="PK198_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRF40638.1"
                     /translation="MSTDGVKVIAIDDTELIDLSKILEQIAEGFKISRITVVHNPKGS
                     ITKDFNRIHEYSIFLTPEDRKDCIARTLEENETPRKMRRWGENSLRTERKLSFYPIYI
                     RDSKIVRIGEVPDDNFHPTGRNVILDTGEIEVWPIDQDGIERRWNFGLDSIGQNLDRI
                     TIQKVDNSYDLFLTHEVTVPKTVWTGGEYDAGNYGNTLLINILGEKKFDFPKSINLVR
                     RCVYLTTSEHKSSLVLDYFAGSGTTGHAVINLNREDGGRRKYILVEMGQYFDTVTKPR
                     IQKVVYSADWKDGKPVGRKGSSHAFKYLRLESYEDALNNLQLRRSAEQQQLLDLGGRF
                     AEEYLLHYWLDTESAGSLLSVAAFERPFDYRLRIIREHEALPTRIDLVETFNYLIGLR
                     VESSQRIRGFPVVSGQNLAG"
     assembly_gap    1244..1456
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1511..2167
                     /locus_tag="PK198_17725"
     CDS             1511..2167
                     /locus_tag="PK198_17725"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRF40639.1"
                     /translation="MKNEFHHNVHNMNDLDWLPDVVAQLPEAQKVRKNYFEIAGFPRW
                     ELVSSNMLAFYFDTEEEHGFKDLFLQSLFDAIEAKLASNKGKADKSFQRELYSYHGDI
                     CVIREFRTSEIKRIDLVIKKQGETGNEENSNSEWAILIENKFENAPLGRIHNDLGHYW
                     ESVKAKNKLGVVLSLYNIKDKSNLEGPGWKYEHLTHQELADAVLANLYISRNLTACIC
                     "
     assembly_gap    2392..2401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2402..2829
                     /locus_tag="PK198_17730"
     CDS             <2402..2829
                     /locus_tag="PK198_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40640.1"
                     /translation="PIEDFSAGKDFIPSGLRFWININSLKERNVLDGAFELYGKENTK
                     YGEEMRSFVKKLEISSSPSIEDGSRGSSNGEFCHLVYYKIKLSTDTNQTFQSCLETVL
                     TEHIFKHPKEYIQKISVKLAELKSGKSGATTPDIAQDQS"
     gene            2832..3998
                     /locus_tag="PK198_17735"
     CDS             2832..3998
                     /locus_tag="PK198_17735"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRF40641.1"
                     /translation="MAKQKKNLHLSDALILHRYLLHQFGVTALEPLAEHLQSSHLEGL
                     DTNNVSKFHHELVARLFGNERLPRQLLYEYDQNIVSHTLAISEHRGQLLHWKYFQYLA
                     LLFTEMYLDKYFRDREGLLRELNEYVDQFNDPFNAGVPNESDYPAPHFKAEELNKIAF
                     WQATGSGKTLVMHVNIRQFQHYARKYGNGQQEINKVLLVTPNEGLSEQHLEEFRLSGI
                     PAVVFSKQTGGAFSGTEVEVIEISKLAENSGDKTVAVEAFETNNLVLIDEGHRGVASD
                     QWKERRDQLSRNGFAFEYSATFGQAVSASAGSKKKELLEEYGKSILFDYSYRYFYHDG
                     YGKDYHILNIADTANEEFTRKYLSGSHFINSNSFSATTPAWRSITSWKNRCGCL"
     gene            3953..>5409
                     /locus_tag="PK198_17740"
     CDS             3953..>5409
                     /locus_tag="PK198_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40642.1"
                     /translation="MAQHYLMEKPLWVFVGSSVNAVRTEQKRETSDVITIIRFFSDFI
                     KDSETSKDHIAQIIGGRDGLLDANNISIFANAFTYIKSKNQSAEELYLDILKTVFNTA
                     NPGANVYLDNLKGAEGELGLRLGDTEDYFGVINVGDEGKLHRLCMEQGIAGMDKDFSD
                     SLFQSINRPGSPINLLIGSKKFTEGWSSWRVSTMGLMNIGRGEGSQIIQLFGRGVRLK
                     GYRFSLKRSSKLDQYQMPEKAVPAFIPALETLNVFGIRADYMQQFKQLLEDEGLPPND
                     GVFEKIVIPVMPTVNLAEHRLKVVKVRDGIDFKKDNILSLGLERFYDSPPVHLDWYPK
                     VQVLDSARRAAPGPEALETHRLSMRNLAFVHWDEVYFELLKFKNERGWSNLSIHREAL
                     PQIMADSSWYVLHIPAVELELKSFRQTRIWQEIAVSLLKNYTDRYYNHHKQRYLGRFL
                     EVKTLEPGDPNFIEEYQLFVERSQKDIIENLHKLK"
     assembly_gap    5410..5419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5420..>5792
                     /locus_tag="PK198_17745"
     CDS             <5420..>5792
                     /locus_tag="PK198_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40643.1"
                     /translation="ALAQGTMEQEVRIGPDFSAFEFLQHLYKPLLYIQDKKYREIVKI
                     QPVALNIGEKRFVDDLRRYYESHPDFFENRKLFLLRNMSRSGVGFFDAGNFYPDFILW
                     LIEGERQYVAFIDPKGLRQVEG"
     assembly_gap    5793..5871
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5872..6061
                     /locus_tag="PK198_17750"
     CDS             <5872..6061
                     /locus_tag="PK198_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40644.1"
                     /translation="NDPLISLSSHIISATFYHQVRHWKDQQGMEDFNRRHVYFQEEQR
                     GVYVGLVLEGMRGEVWVE"
     gene            6391..>6889
                     /locus_tag="PK198_17755"
     CDS             6391..>6889
                     /locus_tag="PK198_17755"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40645.1"
                     /translation="MELQQNDFFRRKGVNIPNVDDIIRKAVEENAKSLKLIKCFLQEI
                     PSEVFGLEHLQTLDLSENNIAAIPPQIAQLKNLKTLVLRKNNISSLPEEIGELSNLSH
                     LILGYNYLNDLPKSFARLQKLRELHLTSNLFTEIPDPIFELRELKVLWLTFNEIKEIS
                     GKISQL"
BASE COUNT         1885 a         1589 c         1615 g         1488 t
ORIGIN      
        1 taagtacttg tcgactgatg gcgtaaaagt aattgcgatt gatgatacgg agttgattga
       61 cttatccaaa atacttgagc agatagcaga aggattcaaa atatccagaa ttacagtagt
      121 ccataaccct aaagggtcaa taactaagga tttcaaccgc atccatgaat actcaatttt
      181 tttaactccc gaagatcgaa aagattgtat tgcaagaact ctggaagaga atgaaactcc
      241 aagaaaaatg agacgttggg gagagaactc tcttcgaaca gaaagaaaat tatcctttta
      301 tccaatttac attcgtgaca gtaagattgt taggataggt gaagtgcctg atgataattt
      361 tcatcctact gggaggaatg taatacttga cacaggagag attgaagtgt ggcctatcga
      421 tcaagatggt attgaaagaa ggtggaattt tggtctagat tcaattggac aaaacttaga
      481 tcgtatcaca attcaaaagg tagataattc atacgacttg tttttaacac atgaagttac
      541 tgttccaaaa acagtttgga ctggaggaga atacgatgca ggaaattatg gtaacacgct
      601 tttgataaat attttaggcg aaaaaaaatt tgatttccca aaatcgataa acttagtgag
      661 acgatgtgtt tatttaacga cttctgaaca caagtcaagc cttgttctcg actacttcgc
      721 cggcagcggc accacgggcc atgccgtcat caacctgaac cgggaagacg ggggcaggcg
      781 caagtacatc ttagtggaga tggggcagta tttcgacacc gtcaccaagc cccgcataca
      841 gaaggtagta tatagcgccg actggaaaga cggcaagcct gtggggcgca agggcagcag
      901 ccatgccttc aagtacctgc gcttagagag ctacgaagac gcgctcaaca acctgcaact
      961 gcggcgcagc gccgagcagc agcaactgct cgatctgggc ggccgcttcg cagaagagta
     1021 cctgctgcac tactggctcg ataccgagag cgccggcagc ctgctcagcg tagccgcctt
     1081 cgagcgcccc ttcgactacc ggctccgcat catccgcgag cacgaagccc tgcccacccg
     1141 catagacttg gtagaaacct tcaactacct catcggcctg cgggtagaaa gctcgcagcg
     1201 cattcgcggc ttccctgtag tgagcggcca aaacctggcc gggnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnngttt gatgtgcagg atgtgtagcc tgtcggagga gacaggtatc
     1501 aagcaaaggg atgaagaacg aatttcacca caatgtacat aacatgaatg acttggattg
     1561 gctgccggat gtagtggcac aattgcccga agcgcagaaa gtgcgtaaaa actacttcga
     1621 gattgcagga ttcccccgat gggagctggt gagcagcaac atgctggctt tttatttcga
     1681 caccgaagaa gagcacgggt tcaaagatct ttttctccaa agcctgtttg atgcaattga
     1741 ggcaaaattg gcaagcaata aaggcaaggc agataagtct tttcaacgcg aattgtattc
     1801 atatcacggc gacatttgcg tcatcagaga attccggacc tcagaaataa aacgcattga
     1861 tttggtcata aaaaaacaag gtgaaacagg taatgaggag aacagcaact cagagtgggc
     1921 cattctgatt gaaaacaaat ttgaaaacgc ccccttgggc cggattcaca acgatctggg
     1981 gcactattgg gaaagcgtaa aggcgaaaaa caaactgggc gttgttctga gtctgtacaa
     2041 cattaaggac aaatccaatc ttgaaggacc aggctggaaa tatgagcatc taacccacca
     2101 ggaactggca gatgctgtct tggccaatct ttatatatca aggaatctga cagcctgcat
     2161 ctgctaatgc tccgggaata tctaaataac ctggcatctc tcagcaaatg gggtgcgcaa
     2221 ataatgcctg aaatggaaaa aatactgccg ctgtaccaac agcatttgga agacataaaa
     2281 aagttgaatg agtgggaagt taatatccac aagtttgtag caagccatct gcttcaagtg
     2341 atggctcaac aggggtttgt tcctaccgat caaaccgtaa cttcctggtc gnnnnnnnnn
     2401 natcctattg aggactttag tgccggcaaa gactttattc cgagtggttt gcggttctgg
     2461 atcaacatca atagcctaaa agaacgcaat gtattggatg gcgcatttga gctgtatggc
     2521 aaggaaaata cgaaatatgg agaagagatg cgcagttttg ttaaaaagct ggaaatctcc
     2581 tccagccctt cgatagagga cggcagcaga ggctcatcca atggagagtt ctgtcatctg
     2641 gtttattaca aaataaaact gagcaccgac acgaatcaga cttttcagtc ctgcctggag
     2701 actgtcctca cggagcatat attcaagcat ccgaaagaat acatccagaa gatttcagtc
     2761 aaactggctg aacttaaatc gggcaaaagt ggagctacaa caccggatat tgctcaagac
     2821 caaagctaac catggccaaa caaaagaaaa acctccacct gagcgatgcg ctgatcctgc
     2881 accgctacct gctgcaccag ttcggcgtca cagccctgga gccgctggcc gagcatttgc
     2941 aaagctccca tctggaaggc ttggacacca acaacgtgtc caaatttcac catgaattag
     3001 tagcgcgcct gttcggcaac gagcgcctgc cccggcagtt gctgtatgaa tacgaccaga
     3061 acatcgtgag ccatacctta gccatcagcg agcaccgggg gcagctcctc cactggaagt
     3121 attttcagta tctggcgctg ctgttcaccg agatgtattt agacaagtac ttccgcgacc
     3181 gcgagggcct gctgcgcgaa ctgaacgaat atgtggacca attcaacgac cctttcaatg
     3241 ctggagtacc taatgagagc gactatcccg ccccgcattt caaagccgaa gaactcaaca
     3301 aaatcgcctt ttggcaggcc accggatcgg gcaaaacctt ggtgatgcac gtcaacatcc
     3361 ggcagtttca gcactacgcc cgcaagtacg gcaacggcca acaggagatc aacaaagtgt
     3421 tgttggtgac gcccaacgaa gggctgagtg agcaacactt ggaggagttt cgcctcagcg
     3481 gcattccggc ggtggtattc agcaagcaaa cgggcggcgc gttttcaggc accgaagtgg
     3541 aggtcattga gatcagcaaa ttggctgaaa acagcggaga caaaaccgta gccgtggaag
     3601 cctttgaaac caacaacttg gtactcatag acgaagggca ccgcggtgtg gccagcgacc
     3661 aatggaaaga gcgccgcgac caactcagcc gcaacggctt cgccttcgag tattccgcca
     3721 ctttcgggca ggccgtgagc gcctccgcag gaagcaaaaa gaaagaactg ctggaagagt
     3781 acggtaaatc catacttttt gattattcgt accgctactt ttaccacgac ggctacggca
     3841 aagactacca catcctcaac atagccgata ccgccaatga ggagttcacg cgcaaatacc
     3901 tcagtggcag ccattttatc aacagcaact cattttccgc gacaaccccg gcgtggcgca
     3961 gcattacctc atggaaaaac cgctgtgggt gtttgtagga agcagcgtca acgccgtgcg
     4021 cacagaacag aagcgcgaga catcggacgt tatcaccatc atccgcttct tcagcgattt
     4081 catcaaagat tccgaaacct ccaaagacca catcgcccag ataatcggcg gccgcgacgg
     4141 cctgctcgac gccaacaaca tctccatttt tgccaatgcc ttcacctata tcaagagcaa
     4201 aaatcagagt gcggaggaat tgtatctgga cattttgaaa accgtattca atacagccaa
     4261 tcccggcgcc aatgtctatt tggacaacct caaaggagca gaaggagaac tcggcctgcg
     4321 cctcggcgat acggaagact acttcggcgt catcaacgta ggcgacgagg gcaagctcca
     4381 ccgcttgtgc atggagcagg gcattgccgg tatggacaaa gacttttccg actccctttt
     4441 ccaaagcatc aaccggcccg gctctcccat caacctgctc atcggctcca aaaagttcac
     4501 cgaaggttgg agcagttggc gcgtcagcac catgggactg atgaacatag gccggggcga
     4561 aggctcacag attatacaac tatttgggcg cggcgtacgg ctcaagggct accgtttcag
     4621 cctcaagcgc agcagcaagc tcgaccagta ccagatgcct gaaaaagcgg tgccggcctt
     4681 cattcccgca ttggaaacgc tcaacgtgtt cggcattcgc gccgactaca tgcagcagtt
     4741 caagcaactg ctcgaagacg agggcttgcc acccaacgac ggcgtatttg aaaaaatagt
     4801 cattccggtg atgcctaccg tcaacctggc ggagcaccgc ctcaaggtcg tgaaagtgcg
     4861 tgacggcatt gacttcaaaa aggacaatat tctcagtctg ggcttggaac gattctacga
     4921 cagcccgccg gtgcatttgg actggtatcc caaagtgcag gtactcgaca gcgcccgccg
     4981 tgccgcacct ggtcctgagg cattggaaac ccaccgtctg agcatgcgca acctggcatt
     5041 cgttcactgg gacgaggtct atttcgagtt gttgaaattc aaaaacgagc gcggctggtc
     5101 caatctgtcc atacaccgcg aagcgctgcc acagatcatg gccgattcat cctggtacgt
     5161 attgcacatc ccggcagttg agttggaact caaatccttc cggcaaacgc gcatctggca
     5221 ggaaatcgcc gtgtcgctgc tcaaaaacta caccgaccgc tactacaacc atcacaagca
     5281 acgctatctg ggccgctttc tagaggtaaa aacattagag ccgggcgatc cgaacttcat
     5341 agaggagtat cagttgtttg tagagcgttc gcaaaaggac atcatcgaaa atctgcacaa
     5401 gctcaaaatn nnnnnnnnng gcgctggcac aaggcaccat ggagcaggaa gtgcgcatcg
     5461 ggccggactt ttcggcattc gagtttttgc agcaccttta caaaccattg ctgtacatac
     5521 aggacaagaa ataccgcgag attgtgaaga tacagccggt ggcgctcaat atcggtgaaa
     5581 aacgctttgt ggatgatctg cggcgctact acgaatcgca tcctgatttt tttgaaaaca
     5641 gaaagctgtt tttattgcgc aacatgagcc gcagcggagt aggctttttc gacgcgggca
     5701 atttttaccc cgacttcatc ctgtggctga tagagggcga gcgccagtat gtggccttca
     5761 ttgatcccaa gggtttgcgt caggtagagg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaatgatcc
     5881 gctgatttcg ctgtcctcgc acatcatttc ggctactttc taccatcagg tgcgccactg
     5941 gaaagaccag caaggcatgg aggatttcaa ccgccggcat gtgtattttc aggaggagca
     6001 acgcggggtg tatgtggggc tggtgctgga ggggatgcga ggagaggtgt gggtggaatg
     6061 aaaaatggcg ctggtcgaga gactggcgcc atcgtgaagt aagatggcaa tactggcgaa
     6121 aagccagtga gccgggatgt acttccacat ccatatcgat tatatactta aggtcccttg
     6181 taaaacaaga gaaaactgct tgcaggattt ttaatccttc ttctatgcgg ggtttttatc
     6241 ccccctttgc agtttctaca agttgtgtag ccttaattgc caatatatgg ctatcgcttt
     6301 aaatagcttt gagaaggata taggcatact actacaagag cagcatgtga aaacgtccag
     6361 ccgcatttcc ggtacaaaaa taagacaatt atggaacttc aacaaaatga tttttttcgt
     6421 cgaaagggag tgaacatccc aaacgttgat gacattatca gaaaagctgt ggaagaaaac
     6481 gctaaaagct taaagttgat caaatgcttt ttgcaagaaa ttccgtctga ggtctttgga
     6541 ttagagcatt tacaaacctt ggatctctcg gaaaacaata ttgccgccat cccgccccaa
     6601 attgcgcaac tcaaaaatct taagactctt gttcttcgga aaaataacat atcttctctc
     6661 ccggaagaaa ttggagaatt gagtaatctg agtcatttaa tattgggcta taattatctg
     6721 aatgatttac cgaagtcttt tgctcgctta cagaaactta gggaacttca tcttacatca
     6781 aatcttttca ccgaaattcc ggacccgatt ttcgagctta gagaattaaa agtactttgg
     6841 ttgacattta acgagatcaa agagatttca ggcaaaatta gtcaacttg
//
LOCUS       DAORLF010000256         6880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5942_length_6880_cov_1.696410, whole genome shotgun sequence.
ACCESSION   DAORLF010000256 DAORLF010000000
VERSION     DAORLF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_6880_cov_1.696410"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK198_17760"
     CDS             <1..261
                     /locus_tag="PK198_17760"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRF40646.1"
                     /translation="NFVNLMYRLGSDIVQKEGIPFDALLPLIQETALKVVDGLPPADA
                     QTGPARRGDSATMQRHLDLLNGQPHLQEMYRLLSEEILKRDV"
     gene            426..707
                     /locus_tag="PK198_17765"
     CDS             426..707
                     /locus_tag="PK198_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40647.1"
                     /translation="MRIVGTIDHPVLKITIFRNDDRLSLKLESGLYEQTYKFRDGEGV
                     SSVEDVYRFVDEVFIAQVSDAMQQMHQSRIAALARCAPATEQDEFDVIL"
     assembly_gap    838..930
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<931..1814)
                     /locus_tag="PK198_17770"
     CDS             complement(<931..1814)
                     /locus_tag="PK198_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRF40648.1"
                     /translation="MKLLLHPFELQYKHTFAISRESRTVQHTLIVELNDGPHSGFGEA
                     TANKYYSTTTEGMMETLESLRPLIESAQWAEPEEFWQHLNALLPGNPFPLAALDEAAN
                     DLHARRQGLPLHKLWGLNAGQLPLCNYTIGIAPIEEMVDKMRELPWPVYKIKLGTEHD
                     LDIIRQLRQHTDAVFRVDANCAWTAAQTIELAPQLRELGVEFIEQPLPAEDTEGMREV
                     FLHCVLPVIADESCREEADVERCHGLFHGINIKLMKCGGLTPARRMIDRARALGMKVM
                     VGCMTESSVGIAAIGQLLP"
     assembly_gap    1831..1923
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1924..2586)
                     /locus_tag="PK198_17775"
     CDS             complement(<1924..2586)
                     /locus_tag="PK198_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40649.1"
                     /translation="MKYIFTAALFLGSLFSALPAHACDCITILTFCESVRPENPVLLG
                     VVTRHYGSGSQLMDVRVLGWIKSPGFSISDITIVGGSSAICLVETNSFMVGDTLLLTP
                     HLLSTPPLEARHLSFNLFGCAVPFLRVNNGVVRGAIRDGLNEAPLSSLISELGRCMEG
                     IVLDDMKLFPNPVGSRFQVQLSGVLFPEQIRFFNVLGQEVVLSSSLDETKSLLTVDTE
                     SLS"
     gene            2684..3721
                     /locus_tag="PK198_17780"
     CDS             2684..3721
                     /locus_tag="PK198_17780"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRF40650.1"
                     /translation="MKTVLYILALLILIVAALVVRTLWYANVFKTFAPQSAGNEQLIT
                     GMIGAEDITMAGDGIALVSSYDRRGVGAGKAVKGAIFRLDLNNTPPTFTDLTAGFDQP
                     DFRPHGISLYTDPADGTRWLFAVNHRAGKHVVEIFEYRDSVLIHSESIESELFKSPND
                     LVGYGKRSFYFTNDHNSSGEDVSHLKDFLVIGTGEIGRYENGKTETLATGIRYANGIT
                     ISPDGKLLYVAACTDRAVYVYNREPFEKIRTIDCGTGVDNLEWDEEGNLWIGAHPKLL
                     MFLGHSKDPAKRSLSQVLQIQLQNPEQPVVKQVYLNDGNPLSGSSVATVWKNKLLVGG
                     VFDDGVLVVER"
     assembly_gap    3883..3911
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3940..5322)
                     /gene="radA"
                     /locus_tag="PK198_17785"
     CDS             complement(3940..5322)
                     /gene="radA"
                     /locus_tag="PK198_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRF40651.1"
                     /translation="MKIKSIYVCTHCGSTSPKWAGKCNACGEWNTIIEEVATPVTTED
                     KKRQLWQSSSGSERNSPKPVLLPDIQAGNTLRLVTPDEELNRVLGGGIVSGSLVLIGG
                     QPGIGKSTLLLQVALGIQAKVLYVSGEESEEQIKMRADRIHQQRSDCYILTETNTSKI
                     LTHALALQPGLLIIDSIQTLSSPLIESTPGSISQVRECAGELQRFAKETNIPVFIIGH
                     ITKEGTLAGPKLLEHIVDAVLQFEGDQHYSYRILRTLKNRFGSTDELGIYEMQQNGLR
                     EVSNPSELLLSQKDEELSGSAIGATMEGMRPMLIETQALVSRAVYGTPQRSATGFDLR
                     RLSMLLAVLEKRCGFQFGQNDVFLNIAGGLRVDDPAIDLAIVSALISSLEDVIVPGHI
                     CFAGEVGLSGEIRAVTRIEQRIQEADRLGFREIYVSKYNIKGLDTGRYAIRVQSIGRV
                     EELYQALFAV"
     gene            complement(5405..6634)
                     /locus_tag="PK198_17790"
     CDS             complement(5405..6634)
                     /locus_tag="PK198_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF40652.1"
                     /translation="MSEKERKISVPKILTEGALHTQAEMLELPVKREKLFIGIPREIT
                     LQESRVALIPSAVATLTAHGHRVLIEMGAGKKSHYSDHEYSEAGAEIAYSPEQVYQAN
                     AIIKVAPPTLDEIEMMRPNQILISPLQLPTLSSEYMDRLRHKRVIALAMEYIKDNSGT
                     FPIVRIMSELAGISAMLTAAELMAGTSGGKGVLLGGISGVPSARVVILGAGIVAEYAT
                     RTALCLGAEVRIFDNDIYKLKRLQNLVGRPLFTSAIDPYILEQELVLADVAIGAMHSK
                     TGRTPIMVGEEIVEKMKPGAVIIDVSIDQGGCFATSEVTSLDRPTFVKHDVIHYCVPN
                     LASRVSRTSSMAVSNILSPMLMRSGTAGSLEHYVLSNPGMRHGIYTYKGYLTNEYLSK
                     RFQIKYTDLDLLMTSSL"
BASE COUNT         1544 a         1813 c         1791 g         1517 t
ORIGIN      
        1 aattttgtca atctcatgta ccgcctcggc agcgacatcg tgcaaaaaga gggcatccct
       61 ttcgacgcgc tgttgccgct catccaggaa accgccctca aagtagtgga cggcctcccg
      121 cccgccgatg cgcaaaccgg ccctgcccgc cgcggcgatt cggccaccat gcaacgccat
      181 ttggatttgc tgaacggcca accgcacttg caggaaatgt accggctgct gtcggaggag
      241 attttgaaaa gagatgtctg aaaattggaa ccggaatcag tgggcggtag gcggtaggcg
      301 gttctcagta ggcggtgcta agcgcgagga acactcaata tcaggaattt tgaatcagga
      361 atcggaaatc ggaaatcagg aattttgaat cagaaatccg aaatccgaaa taaataagtt
      421 gagacatgcg catcgtaggc accattgacc atcctgtact gaaaatcacc atcttccgca
      481 acgacgaccg gctctcgctc aagctcgaat ccggcctgta cgagcaaacc tacaaattcc
      541 gcgacggcga gggcgtttcc tctgtagaag acgtgtatcg ctttgtagat gaggtattta
      601 tcgcccaagt ctccgatgcg atgcaacaaa tgcaccaatc cagaatagcg gccctcgccc
      661 gctgcgcccc ggcgacagag caggatgaat ttgatgtgat tctgtaacag cgactttagt
      721 cgcccaccct ccgcatgcac tcccgcgtac cggcgacttc agtcgccgac ccacacacaa
      781 aacctttcgg tggctaaagc cgccggtacg cgctacctca acgccgcgcc cgtacccnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcaacaatt gcccgatggc ggcaatgcct
      961 accgaggact cggtcataca tccaaccatc actttcatgc ccagtgcgcg ggcgcggtct
     1021 atcatacgcc gagccggcgt gagaccccca catttcatca gtttgatgtt gatgccatga
     1081 aaaagaccgt gacagcgctc cacgtcggct tcttcgcgac aactttcgtc ggcgatgacc
     1141 ggcaatacgc aatggaggaa cacctcgcgc atgccctctg tgtcttcggc gggcagcggc
     1201 tgctcaatga attccacgcc cagttcgcgc agttggggcg ccagttcgat ggtttgtgca
     1261 gcggtccagg cgcagttggc gtccacccgg aagacagcgt cggtatgttg acgtagttgg
     1321 cgaatgatgt ccaaatcgtg ttcagttcct aacttgattt tatacaccgg ccagggcaac
     1381 tcgcgcattt tatccaccat ttcctctatg ggcgctatgc cgatggtgta gttgcacaac
     1441 ggcaattgcc cggcgttgag tccccagagc ttgtgcaatg gcagtccctg gcggcgggcg
     1501 tggagatcgt tggcggcttc gtccagagcg gccagcggaa agggattacc cggcaggagc
     1561 gcgttcaggt gttgccaaaa ctcctcaggt tcagcccatt gtgctgattc gattagagga
     1621 cgcaggcttt ccaatgtttc catcatgccc tcggtggtag tgctgtagta tttgttggcg
     1681 gtggcctctc cgaagccgga atgcggaccg tcgttcagtt ctacaatgag ggtatgctgt
     1741 acggtgcgcg actcgcgcga gatggcaaat gtgtgtttgt attgcagttc aaacggatgc
     1801 aagagaagtt tcataaggca ttgaattgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnngctgagc gattcggtat ccactgtgag caggcttttt gtttcgtcca aacttgaact
     1981 cagtacaact tcctgcccca acacattaaa aaacctgatc tgttcgggga ataatactcc
     2041 cgaaagctgt acctgaaacc ggctgccgac cggattggga aataatttca tatcatcgag
     2101 tacaataccc tccatgcacc ttcccagctc tgaaataaga gaggataaag gcgcctcatt
     2161 cagtccatca cgaatagcac cccgaactac cccgttgtta acccgcaaaa acggaaccgc
     2221 acacccgaag aggttgaaag aaagatgtct ggcctccaaa ggcggggtgg ataacaagtg
     2281 cggcgtcaaa aggagcgtat ctcccaccat aaagctgttt gtttctacta agcaaatagc
     2341 gctacttcca ccgacaatgg tgatgtctga aatcgaaaat ccaggcgatt taatccatcc
     2401 tagcactctg acgtccatta gttggctgcc ggagccataa tgccgggtca caacacccaa
     2461 taaaaccggg ttttccgggc gtacgctttc gcaaaaagtg aggatggtaa tgcaatcgca
     2521 agcatgagcc ggcagtgccg aaaaaagtga acctaagaaa agagcagcgg taaaaatgta
     2581 tttcataagc atgttttttt gaatcgacaa gtaagacgca cctgataagt aggtacgttg
     2641 gcggcaagag tgtaaatttg tctttcataa aaatctcgtc accatgaaaa ccgtactcta
     2701 tattctggcc ctgctcattt tgatcgtggc cgcattggtc gttcgcaccc tgtggtacgc
     2761 caatgttttt aaaacgttcg cgccccaatc agcaggcaat gagcaactta tcacaggtat
     2821 gattggcgct gaagacatca cgatggccgg agacggcatc gcgttggtgt cgtcctatga
     2881 tcgccggggc gtgggtgccg gtaaggctgt caaaggtgct attttccggc tcgacctcaa
     2941 caacacgccg cccactttta cagacctgac cgccggtttc gatcagcccg atttccgccc
     3001 gcacggcatc tcgctttaca ccgaccccgc cgacggcacg cggtggttgt tcgccgttaa
     3061 tcaccgggca gggaaacatg ttgtggaaat atttgaatac cgggactccg tcctcatcca
     3121 ctccgaatct attgaaagcg agttatttaa aagccccaat gatctggtgg gctatggcaa
     3181 acgctcgttt tattttacca acgaccacaa cagcagcggc gaggacgtgt ctcacctgaa
     3241 ggattttctg gtcatcggca cgggagaaat cggcaggtac gaaaacggca aaacggaaac
     3301 gttggctacg ggcattcgct acgccaacgg catcaccatc agccccgatg ggaaactgct
     3361 gtatgtggcg gcctgcaccg accgggccgt gtatgtttac aatcgggagc cgtttgaaaa
     3421 aatacggacg attgactgtg gcaccggcgt ggacaatttg gagtgggatg aagagggcaa
     3481 tttgtggatt ggcgcgcacc ccaagctgct tatgtttttg gggcattcca aagacccggc
     3541 caaacgctct ttgtcacaag tgcttcaaat tcaattgcaa aacccggagc agcccgtagt
     3601 gaaacaggtc tatctcaacg acggcaatcc actttccggc tcttctgtgg cgacggtttg
     3661 gaaaaacaaa ctgctcgtag gcggtgtgtt cgatgacgga gtgctggtgg tggagcggtg
     3721 aagtcgcaga gctgttcatc tgccttcggc ggtaatctgc gttgcagttc atctgcgtgt
     3781 ggtaatcggg agccgaaacg cgaatttttt aacgcgaaat ttagcgaagg gggcgaatct
     3841 ccgcgaaagc gctaaggaac cggcaggcgg ttttcagggg ggnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn ncccgaaacc ttgaacccga aacccgacct cacaccgcaa aaagcgcctg
     3961 atacaactcc tctaccctgc cgatgctctg cacccgaata gcgtagcggc ctgtgtccaa
     4021 acctttgata ttgtatttgg aaacataaat ttcgcggaag cccagccggt cggcctcctg
     4081 aatgcgctgc tcaatgcggg tgacagcccg aatctcgccc gaaagcccca cctcgccagc
     4141 aaagcaaata tggccgggta cgatcacgtc ttccaatgag gaaatgagtg cgctgacgat
     4201 ggccaagtct atggccgggt catccacccg cagcccgccg gcaatgttga gaaagacgtc
     4261 gttttggcca aactggaacc cgcaacgctt ctccaatacc gccaataaca tactcaggcg
     4321 cctcaaatca aaacccgtgg ccgagcgctg tggcgtgcca tacaccgccc ggctgaccaa
     4381 tgcctgcgtt tcgatgagca tgggccgcat gccttccatc gtggccccga tggcgctgcc
     4441 gctgagttct tcatctttct gagaaaggag cagttcggag gggttcgata cctcgcgaag
     4501 gccattttgc tgcatttcgt aaatgccgag ttcgtcggta gagccgaagc ggtttttcag
     4561 cgtgcgcagg atgcgatagg agtagtgttg gtcgccttca aactgaagca cggcatccac
     4621 gatgtgttcc agcaatttgg ggccggccag cgtgccttct ttggtgatgt ggccgatgat
     4681 gaacaccggg atattggttt ctttggcaaa acgctgcaac tcgccggcac actcgcgcac
     4741 ctgcgaaatg ctgcccggcg tgctctcgat gagcggcgag gacagcgttt gtatggaatc
     4801 aataatgagc aggcccggct ggagcgccag cgcgtgcgtc agtattttgg aggtatttgt
     4861 ctctgtaagg atgtagcaat cggagcgctg ctgatggatg cggtcggcgc gcattttgat
     4921 ctgttcctcg ctttcttcac cggaaacgta cagcactttg gcctgaatgc ccagcgctac
     4981 ctgcaacaaa agggtggact tgccgatgcc gggctgcccg ccgatgagca ccaaagagcc
     5041 gctaacaatt ccgccgccca atacgcgatt gagttcctcg tcgggagtga ccaagcgcag
     5101 tgtattgcct gcctgaatat cgggcaagag cacgggtttg ggagagttgc gctccgagcc
     5161 ggaggagctt tgccacagtt ggcgtttctt atcttcggtg gtgacgggtg tggccacttc
     5221 ctcaatgatc gtgttccatt caccgcaggc attgcacttg ccggcccatt tcgggctggt
     5281 gcttccgcaa tgggtgcaga cgtatatgct ttttattttc atgaattttt ctgtgcttat
     5341 agtgaagggg tgaccaaggc atggaatttc aggcgaagtc accccttcac tatactaccg
     5401 acatttataa actggaggtc atcagcaaat ccaaatcagt atatttgatc tgaaagcgct
     5461 tgctcaggta ctcgttggtc agatagcctt tgtaagtgta aatgccgtgc cgcatccccg
     5521 gattgctgag cacatagtgt tcgaggctgc ccgccgtgcc ggaacgcatg agcataggcg
     5581 acaaaatatt gctcaccgcc atggaagagg tacgcgacac ccgcgatgcc aaattgggca
     5641 cgcaatagtg aatcacatcg tgtttgacga aagtcgggcg atccaatgaa gtcacttccg
     5701 aagtagcaaa acagccgccc tggtcaatgc tcacatcgat gatgacggcg ccgggtttca
     5761 tcttttctac aatttcctct cccaccataa taggcgtgcg gcccgttttg gagtgcatgg
     5821 ctccgatggc tacatcggcc aacacgagtt cctgctccag aatgtacggg tctatggcag
     5881 aggtaaaaag cggccggcct acgagatttt gcaagcgttt gagcttgtaa atgtcgttgt
     5941 cgaaaatgcg gacttccgcg ccgagacaca acgctgtgcg ggtagcgtat tctgccacga
     6001 tgcctgctcc caaaatgacc acccgcgcac tgggcacacc ggagatgcca cccagcaaaa
     6061 cgcccttgcc gccactggtg cctgccatca gttcggcggc ggtgagcatg gcgctgattc
     6121 ccgccaactc actcataatg cgcacgatag gaaaagtgcc ggagttgtct ttgatgtact
     6181 ccatagccag cgcaatgacc cgtttgtgcc gcagccggtc catatactcg ctgctgaggg
     6241 taggcaattg caggggcgaa atgagtatct gattagggcg catcatttcg atttcatcca
     6301 aggtaggcgg ggccactttg attatggcat tggcctgata cacctgttcc ggcgagtagg
     6361 caatttctgc gccggcctcc gaatactcgt gatcggaata atgagatttt ttgcccgcgc
     6421 ccatctcaat gagtacccga tgcccatgcg ccgtcagcgt ggccacagca gagggaatca
     6481 gcgccacgcg gctttcctgc aaggtgatct cgcggggaat accgatgaac agcttttcgc
     6541 gcttgacggg caattccagc atctcggcct gcgtgtgcaa ggcgccttcc gtcaaaattt
     6601 tgggtacgga tattttcctt tctttttcgc tcataatagt ttagctgcga gccgcggggt
     6661 acgagcctgt atgagatgaa acccgcagct cgcagcccat aactcacaat ttatcttatc
     6721 aaggtcaaat cgcctgattt ttcgagcacc ggcccttccg ggtagcggaa tttcacccga
     6781 tacacataca cgtctatcgg agcaggactg ccgttgtgta cgccattcca gccgatgttg
     6841 tcgttgctct tgccgttata caccacctgt ccccagcggt
//
LOCUS       DAORLF010000257         6874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5950_length_6874_cov_2.095175, whole genome shotgun sequence.
ACCESSION   DAORLF010000257 DAORLF010000000
VERSION     DAORLF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_6874_cov_2.095175"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK198_17795"
     CDS             <1..433
                     /locus_tag="PK198_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRF40653.1"
                     /translation="CIQYGRELLEKPIELLDGVEEVLHALYGRYRLVMATKGDLLDQE
                     RKLKKSGLERFFHHIEIMSEKKEPDYQKLIRHLDIHPAEFLMVGNSLKSDVLPVLDIG
                     GHGIHVPYHITWEHEKVELQIEHPNFRQVAHIREILPFLLP"
     gene            430..1065
                     /locus_tag="PK198_17800"
     CDS             430..1065
                     /locus_tag="PK198_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HRF40654.1"
                     /translation="MKRTLPLETWARREHYLFFKDFDEPFFGVNLRVDCTAAYAASRR
                     LGHSFFLSYLHKALRAANETEAFRIRIENGEVVIFDRINASPTVGRPDGTFAFSYMQY
                     HPDFEVFAAAGQAEIERVRHSSGLCLGDEKPDVIHFSSVPWIDFNGISHARHFSLRDS
                     VPKITFGKMIESGDRKLMPVSIHAHHGLMDAWHVGQFLERFEELMSGVSQG"
     gene            1307..2335
                     /locus_tag="PK198_17805"
     CDS             1307..2335
                     /locus_tag="PK198_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRF40655.1"
                     /translation="MKQFAFLSMLAMMLFSGCKQSNETGAAASSSRPEYALVIHGGAG
                     TIRREDMSPEKEAAYTAALNQALDIGEEVLRNGGSAMDAVEAVIVFLEDTPLFNAGKG
                     AVFTNDGRNELDASFMNGATREAGAVAGVTIVKNPIRLARKVMENSVHVMMAGKGAEL
                     FAKEQGLDTVPPEYFFTQERWDGLQRAIDRENKSTGALDPAHNDSKFGTVGCVALDKS
                     GNIAAGTSTGGMTNKRYGRVGDAPIIGAGTYADNATCGVSATGHGEFFIRYTVARDIA
                     ALMEYKGLSLNEAADMVVNKKLVEAGGEGGVIALDRNGNVAMPFNSAGMYRGYAKPGK
                     REVGIYKE"
     gene            2544..3158
                     /locus_tag="PK198_17810"
     CDS             2544..3158
                     /locus_tag="PK198_17810"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRF40656.1"
                     /translation="MILTIHPQNPDQRKIAQVAEMLRKHGVVIYPTDTVYAIGCDMMS
                     KEAVERLCRIRHLDPQKAMLTLICKDLSQVAEYAAQIDTPVFKLMRRNLPGPFTFILP
                     AGSKTPKLFINRKKTIGVRIPDNAILMALVEELGHPILTASLKTNDDVVEYFTDPEDL
                     HDQYENLVDAVIDGGPGGIVPSGLIDCTGQEPELIREGAGELKW"
     gene            3237..>3525
                     /locus_tag="PK198_17815"
     CDS             3237..>3525
                     /locus_tag="PK198_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40657.1"
                     /translation="MQILRDKTAVITGAASGIGRALALQLAEAGCRLALCDHNDAELN
                     ETRKMAIEKGAVVIAVPFDVSNREDMRNFATEVLQVFGSVDIVVNNAGLTLM"
     assembly_gap    3526..3556
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3557..4040
                     /locus_tag="PK198_17820"
     CDS             <3557..4040
                     /locus_tag="PK198_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF40658.1"
                     /translation="DFQRVWEVNFMGVLHGCQTFLPHLRERPEAALVNVASIFSTAAY
                     PYQGPYVASKFAVRGLTETLRQELAGTCVAVTVVMPGGIKTNIVNNIATTDIVAKEKF
                     ARRFDKSAPTTAEEAARCIIEGIRRKKQRVLIGKDAKIMDIIARIFPHRYEQVIKRFV
                     "
     gene            4186..4788
                     /locus_tag="PK198_17825"
     CDS             4186..4788
                     /locus_tag="PK198_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40659.1"
                     /translation="MLTKEELEAVYKKFDDDKIVKLAMDESKSLREDAVPILEQEIIR
                     RNLDDKLLEWIKWERNFFKGAELESIKAVIKNSQCTECGGKDSEIKGFNIHYYSMFDS
                     TFDANIIVCERCGKRARKKSYIKTATLGWISFYGIISVPLYFIGEIIASFSRENTSKE
                     IIESFIFENTGLIREYGIENIRDLIYNSNLVQMKSDEITE"
     gene            4926..6356
                     /locus_tag="PK198_17830"
     CDS             4926..6356
                     /locus_tag="PK198_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF40660.1"
                     /translation="MTTTTPYLTETAICALFEQQLAKAPRVALTTARQRLEKIKKIED
                     WIENEANVRALSEAMHKDFRKSSFEVWATEIGIVKQAVVAVRRNLRHWMQDEPVDGSL
                     ALAGMSSWIRYEAKGACLIIAPWNYPFNLAIMPLVYAIAAGNTVILKPSELTPHTSAL
                     IAAMLDELFPPEEVAVVEGDADTAQALLQLPFHHIFFTGSPAVGKIVMAAAARHLSSV
                     TLELGGKSPVVLDETADIPYHAEKLVWAKLMNNGQTCIAPDYVLVHASKQDQLLAELK
                     KALQKLYGADPRQSPDLARVVNDRNFQRLRRLLDDAVSKGARVAIGGNMVEAERYIAP
                     TVLTGLNDEMDIQHEEIFGPILPVIPFQNREEALAFIRRRPKPLDMYIGSRSQVNIRY
                     FLENTSAGSTVINEYMTSFSNTNLPFGGVNNSGLGKSLGVHSFREFSNARGLMRRHWL
                     FAGLSMIQPPFSDVKMKLLKFFYKLV"
     gene            complement(6428..>6874)
                     /locus_tag="PK198_17835"
     CDS             complement(6428..>6874)
                     /locus_tag="PK198_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF40661.1"
                     /translation="LTAKDQTEDVLAGFASGGNDYLRKPFSMEELIVRLKNLIALSNP
                     DNQPATDMEPVMLGQFRFNPTRQELLLGEQAKRLSHRETQLLEMLAQNINKDVLRKEI
                     LLLLWGNDSYFNSRNVYIARLREYLRPDPNVEIITLKGIGYRLVAV"
BASE COUNT         1671 a         1869 c         1858 g         1445 t
ORIGIN      
        1 gtgcatccaa tacgggcgcg aactgctgga aaaacccatc gaactgctcg acggtgtaga
       61 ggaagtgctg cacgccctgt acggccgcta ccgcctcgtg atggccacca aaggcgacct
      121 gctcgaccag gagcgcaagc tcaaaaagtc gggcctggag cgcttttttc accacatcga
      181 aatcatgtcg gagaaaaaag agcctgatta ccagaaactt atccggcatt tagatatcca
      241 tcccgccgag tttctcatgg tcggcaattc cctcaaatcc gacgtgctgc ccgtgctcga
      301 catcggcgga cacggcatcc acgtccctta ccacattacc tgggaacacg aaaaagtgga
      361 actgcaaata gagcatccca atttccggca ggtggcacac atccgcgaaa ttttgccatt
      421 tttgctgcca tgaaacgcac actccctctc gaaacctggg cgcgccgcga gcactacctc
      481 ttcttcaaag acttcgacga gccgttcttc ggagtcaatc ttcgggtgga ctgcaccgcc
      541 gcctacgccg cctcccgccg cctcggccac tcgtttttcc tctcctacct tcacaaggcg
      601 ctccgggccg ccaacgaaac cgaagccttc cgcatccgca tcgaaaacgg ggaggtagtc
      661 atcttcgacc gcatcaatgc ttcgcccacc gtcggtcgcc ccgacggcac cttcgccttc
      721 tcgtacatgc agtatcaccc tgatttcgag gtgtttgccg ccgccgggca ggcagaaata
      781 gagcgcgtgc gccacagcag cggcctctgt ctcggcgacg aaaaacccga tgtaattcac
      841 ttctcgtccg tgccctggat agattttaac ggaatttcgc acgcccggca tttttccttg
      901 cgcgattccg tgccgaagat tacctttggc aaaatgatcg aatccggcga ccgaaagctg
      961 atgccggtct ccatacacgc gcaccacggg ctgatggacg cttggcatgt ggggcagttt
     1021 ttggagcggt ttgaggagtt gatgtcaggg gtgagtcagg ggtgagtagg tgaggggtga
     1081 gtaggtgagt gggtgagtag gtgaggggcg tgtccccact cccgttgatg cctgtgtggg
     1141 gccgggtgcc tcagtcgctc ctcctgcgtc gtcactacct tcggccccct cacgcgacgg
     1201 ggcaagagct gttggctttt tgccggttta tgcggtgata aagctaacag ctctgcggtc
     1261 tgagcgggcg gctgccacct tcatttataa agacaatcaa tgccttatga aacaatttgc
     1321 ctttttgagt atgctggcca tgatgctttt ttcgggctgc aaacagagca atgaaaccgg
     1381 cgccgccgcc tcgtcttcgc gccccgaata cgccctcgtg atacacggcg gagcgggtac
     1441 cattcggcgc gaggatatga gtcctgaaaa ggaagccgcc tacaccgccg ccctcaacca
     1501 agctttggac ataggcgagg aggtgctgag aaacggcggc tcggcgatgg atgccgtgga
     1561 agctgtcatt gtgtttttgg aagacacgcc gctgttcaac gcgggcaagg gcgcggtttt
     1621 taccaacgac gggcgcaatg aactcgatgc ttcctttatg aacggcgcta cccgcgaggc
     1681 cggggcggtg gcgggtgtca ccattgtgaa aaacccgatt cgactggcgc gcaaggtcat
     1741 ggagaactcg gtgcatgtga tgatggccgg caagggcgcg gagttgtttg ccaaagaaca
     1801 gggcttggac acggtgccgc cggaatattt cttcacacag gagcgctggg acgggctgca
     1861 acgcgccatt gatcgcgaaa acaaaagtac gggcgcactc gatccggcgc acaatgattc
     1921 aaaattcggc acggtgggtt gtgtggcttt ggataaaagc ggtaacattg cggcgggtac
     1981 ttctaccggc ggcatgacga ataagcgata cggcagggta ggggacgcgc ccatcatagg
     2041 ggcgggtact tatgccgata atgcgacctg cggcgtatcg gctacggggc atggcgagtt
     2101 ctttattcgc tacaccgtgg cccgcgacat tgccgcgctg atggagtaca agggcttgtc
     2161 actgaacgaa gcggcggata tggtggtgaa caagaaattg gtggaggccg gcggcgaagg
     2221 gggcgtcatt gcgctggacc gcaacggcaa tgtggccatg cctttcaatt cggcgggcat
     2281 gtaccgcggc tacgccaagc ccggaaagcg ggaggtaggg atttataagg agtaaatctg
     2341 cgaagcagtt tatctgcatg cggagagggg cgcggtgcga agcgcggaat tttgaacgcg
     2401 aatcaacgca aaatacgcga atcaacgcga acgagaggga aacccggaac ccgaaaccgg
     2461 taggcagtgg gcggtaggca gtcagcggtt tcaacccgga actcggaacc cgaaacattg
     2521 aacacgaaac aaaagacaac gtaatgatac taacgattca tcctcaaaat cctgaccaga
     2581 gaaaaattgc ccaggtggcg gaaatgctgc gcaagcacgg cgtcgtcatc tatcctaccg
     2641 acacggtgta tgccatcggc tgcgacatga tgagcaaaga ggccgtagag cgcctgtgtc
     2701 gcatccggca tctcgatccg caaaaagcca tgctcacgct gatctgcaaa gacctcagtc
     2761 aggtagccga atacgccgcg caaattgata cgccggtgtt caagctcatg cgccgcaacc
     2821 tgccggggcc gtttaccttc atcctgcccg caggcagtaa aacgcccaaa ttgttcatca
     2881 atcgcaaaaa gaccatcggg gtgcgcatcc ccgataatgc cattttgatg gcgctcgttg
     2941 aggaactggg gcaccccatt ctcaccgcat cgctcaaaac caatgacgac gtggtggagt
     3001 acttcaccga tcccgaagac ctgcacgacc aatacgaaaa cttggtggat gcggtcattg
     3061 acggcgggcc gggcggcatc gtaccttccg gtctcataga ttgcaccggc caggaaccgg
     3121 aattgatccg cgaaggcgcc ggcgaactga aatggtagcc actcacccac tcaccactca
     3181 cccactcacc actcacccac tcacccactc acccactcac cactcaccac tcacccatgc
     3241 aaatacttag ggacaaaaca gccgtcatca ccggcgctgc ctccggcatc ggcagggcgc
     3301 tggcactcca attggccgaa gccggctgcc gcctcgccct ctgcgaccac aacgacgcag
     3361 aactcaacga gacccgcaaa atggccattg aaaaaggcgc tgtggtaata gccgtgcctt
     3421 ttgacgtgtc gaatagggaa gacatgcgca acttcgccac cgaagtgctg caagtgtttg
     3481 gcagtgtgga cattgtcgtc aacaacgccg gcctgacact gatgcnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnagat tttcagcgcg tgtgggaggt taattttatg ggcgtactgc
     3601 acggatgcca gacgtttttg ccccatctca gagagcgccc cgaagcggcc ttggtcaacg
     3661 tggccagtat tttcagtacg gcggcttatc cgtatcaggg tccgtatgtg gcctccaaat
     3721 ttgccgtgcg cggcctcacc gaaacgctcc ggcaggaact cgccggcact tgcgtagcgg
     3781 tcacggtggt gatgccgggc ggcatcaaaa ccaacattgt gaacaacatc gccactacgg
     3841 acattgtagc caaggagaag ttcgcccgca ggttcgacaa gtcggcgccc actactgctg
     3901 aagaggcggc tcgttgcatc attgagggca ttcgccgcaa aaagcagcgc gtcctcatcg
     3961 gcaaagacgc gaaaataatg gacatcatag cgcgtatttt tccgcatcgg tatgagcagg
     4021 tcattaagcg gtttgtgtag ggaggttttt tggggttttg ggttggagtg gctgctggct
     4081 gttagctgct ggctgttagc tgctggctga attcggatgg gaacgggctg ccgcacacgg
     4141 gttccgggtt gagtacagtt gaagttattt gtaagcttaa aagaaatgct aaccaaagaa
     4201 gaattggagg cggtttataa aaaatttgac gatgataaaa ttgtcaaatt ggctatggat
     4261 gaaagtaaaa gtttgcgtga agatgcggtg ccgattcttg agcaggaaat tatacgcaga
     4321 aatcttgacg ataaattatt agagtggata aaatgggaga gaaacttctt caaaggtgca
     4381 gaattggaaa gcattaaggc tgtaataaaa aactctcaat gtacagaatg tggaggcaaa
     4441 gattcagaga taaaagggtt taatatccac tattactcaa tgtttgactc gacttttgat
     4501 gcaaatatta ttgtctgtga gaggtgtgga aaaagagcga gaaaaaaaag ttatataaaa
     4561 acagcaactt taggatggat ttctttttat ggaataatta gtgtcccgct gtactttatt
     4621 ggagaaatta ttgcaagttt cagcagagaa aatacaagca aagaaatcat tgaaagtttt
     4681 atttttgaaa atacaggatt gataagggaa tatggaattg aaaatattag agatttgatt
     4741 tacaacagta atttggtaca aatgaaatca gatgaaataa ctgaataaaa atgccaccgg
     4801 ccgataaact gccgaaggta aattacggcg cagccgataa actgcgaacc gcgcaaggcg
     4861 cgggaagccg ataaactgca aagcagctca caacgaagtg aattacaaat attgatacag
     4921 acatcatgac caccaccact ccttatctca ccgaaacggc catttgcgcc ctctttgagc
     4981 aacaactcgc caaggccccc cgcgtggccc tcaccaccgc ccgtcagcga ttggaaaaaa
     5041 tcaaaaaaat agaagactgg atcgaaaacg aagccaatgt gcgtgccctc tctgaggcca
     5101 tgcacaagga ttttcgcaaa tcctccttcg aagtgtgggc caccgaaatc ggcatcgtca
     5161 agcaggcggt cgtggccgtt cggcgcaacc tgcgccactg gatgcaagac gaacccgtgg
     5221 acggctccct cgccctcgcc ggcatgtcgt cctggattcg ctacgaggcc aaaggcgcgt
     5281 gtctgatcat cgcgccctgg aactacccgt tcaacctcgc catcatgccc ttggtgtatg
     5341 ccatcgccgc cggcaatacg gtcatcctca agccctccga actcacgccg cacacttcgg
     5401 cgctcattgc ggccatgctc gacgagctgt tcccgccaga agaagtagcc gttgtagagg
     5461 gcgatgcgga taccgcccaa gccctgctgc aactgccttt tcatcacatt ttcttcaccg
     5521 gcagtccggc cgtgggcaaa atcgtgatgg ccgcagcagc ccggcacctc agttccgtta
     5581 cgctcgaact cggcggcaaa tcgcccgtcg tactcgatga aactgccgac atcccctacc
     5641 acgccgaaaa actcgtgtgg gccaaattga tgaacaacgg ccaaacctgc atcgcgcccg
     5701 actatgtact cgtacacgcc tccaaacaag accaattgct ggccgaactc aaaaaagccc
     5761 tccaaaagct atatggcgcc gatccccggc aatcgcccga tctggcccgc gtggtcaacg
     5821 accgcaactt ccagcgcctc cgccgcctcc tcgatgacgc cgtctccaaa ggcgcccgcg
     5881 tggccatcgg cggcaacatg gtcgaggccg agcgctacat tgctcccact gtactcaccg
     5941 gcctcaacga cgaaatggac atccagcacg aagaaatttt cggccccatc ctgcccgtca
     6001 tccctttcca aaaccgcgaa gaagccctcg ctttcattcg ccgccgtccc aaaccattgg
     6061 acatgtacat cggcagccgc agccaggtca atatccgcta ctttttagaa aacacctccg
     6121 ccggcagcac ggtcatcaac gagtacatga ccagtttcag caataccaac ctgcccttcg
     6181 gtggcgtcaa caacagcggt ctgggcaagt cgctgggcgt tcacagtttc cgcgagttct
     6241 ccaacgcccg cggcctgatg cgacggcatt ggctgttcgc aggcttgagc atgattcagc
     6301 cgccgttcag cgatgtgaaa atgaaattgc tgaagttttt ctacaagttg gtgtgaggtt
     6361 gtagcctgga ggttgtggca aatatattga tgcaagtatt tgactatcag ataaaaatga
     6421 aactttctta cacggcaacc agccgatagc cgatgccttt cagcgtaatg atctctacgt
     6481 tgggatcggg acggaggtac tcgcgcaggc gggcaatata cacgttgcgg gaattgaaat
     6541 aggagtcatt gccccaaagg aggaggagta tttccttgcg cagcacgtct ttgttgatgt
     6601 tttgggccag catttccaaa agttgcgttt cgcgatgaga gagtcttttg gcttgttctc
     6661 ccaacagcag ttcctgtcgg gtggggttga accggaattg ccccaacatg acaggctcca
     6721 tatctgtcgc cggctgattg tccgggttgc tcagtgcaat gagatttttc agtcgcacga
     6781 tgagttcctc catgctgaag ggctttcgca ggtagtcgtt gccgccggaa gcaaaaccgg
     6841 ccaacacgtc ttcggtttga tctttggcgg tgag
//
LOCUS       DAORLF010000258         6873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5951_length_6873_cov_1.739513, whole genome shotgun sequence.
ACCESSION   DAORLF010000258 DAORLF010000000
VERSION     DAORLF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5951_length_6873_cov_1.739513"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>2547
                     /locus_tag="PK198_17840"
     CDS             189..>2547
                     /locus_tag="PK198_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRF40662.1"
                     /translation="MSKGKAGIRPEGRTDDNGGQIIGLQGMYEDYFLDYASYVILERA
                     VPAIEDGLKPVQRRILHSMFDLDDGRYHKVANIIGYTMQFHPHGDAAIGDALVNMGQR
                     DLLIDCQGNWGDVRTGDSAAAPRYIEGRLTKFALEVTFNPQTTDWQLSYDGRKKEPVT
                     LPVKFPLLLAQGVEGIAVGLSTKILPHNFIELIKASIKILQGKKVKIYPDFPTGGSID
                     VSDYNSGARGGKVKVRAKIEVVDKSTLAVRDLPFGVTTGSLIESIVKANDKGKIKIKK
                     VVDNTAQLVEVHIELAPGVSPDVTVDALYAFTDCEVSISPNACVIVEDKPVFTTVEDI
                     LEISTNNTRELLRSELEIKKHELQEKWHFASLEKIFIEKRIYHEIEECESWEEVMATI
                     YRELARYVATPSDPPQPGDARLPLHRNIDDDDITRLTEIRIKRISKYNAFKADELIAQ
                     IEADLRQVQHDLEHLTEFAVAYFERLLEKYGKGRERRTLITEFDTIQATEVVANNAKL
                     YVNRAEGFIGSGLKKDEFVCDCSDIDDIIVFRRDGKFQVSRISDKVFVGKDIIHVAVW
                     KKNDERTTYNMAYSDGKSGRSFVKRFNVTAITRDREYDLTQGNPNSKLLYFSANPNGE
                     AEIVVVQLSAGATAKIKIFDYNFAELEIKGRSSQGNILTKYPVKKVTLKEAGKSTLGA
                     QHVWIDEVSGKLNTDNRGRLLGDFDTGDQLFLLYKDGTYEVADVDMSRRFDPDELLHL
                     GKFDPEMVINAVHFDGNKGWTMVKRFRVETTSTGQRFSYLSEHKDS"
     assembly_gap    2548..2565
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2566..2855
                     /locus_tag="PK198_17845"
     CDS             <2566..2855
                     /locus_tag="PK198_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536074.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRF40663.1"
                     /translation="FASIKKEPRIEYSVKIKGKKIQGEVNLADFIDVKGWKAMGNKLS
                     DQKLSSVKELEDPTLFQTPDSNAAEVPLPEKFHAGDSLEFDMGKNGQGSLF"
     gene            complement(2859..3668)
                     /locus_tag="PK198_17850"
     CDS             complement(2859..3668)
                     /locus_tag="PK198_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40664.1"
                     /translation="MEEPKLTPEEELRMENQLKALDLEINHGAANFLSDDLPPEITKM
                     FLENVANFEAAHANARMVPVREFAGIPEQPGYKEVAEEDLEAHIQSLLEQLEAAGVII
                     DRPEHLRPRGYYRFLTEDFLNSEMTDYTAPGMIHGFSYSDFHHDGPEFIREHVEETLL
                     ELLNLNRDVEGEWISETCRDQSEAISKAEVLQRIHIFRSKYTEIKPIAFREQQVQRTE
                     VGMYFFFLVAWEGTPIHGGEPERHEGLGVSQIEYEDGEWMVQGIMMPGFEF"
     gene            complement(3814..>4301)
                     /locus_tag="PK198_17855"
     CDS             complement(3814..>4301)
                     /locus_tag="PK198_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40665.1"
                     /translation="INLLSWQTASETNNRGFEVQRSDDGVRWQEIGFVNGYGTTTETQ
                     HYTLIDKEPLGGTNYYRLRQIDFDGTASLSNIVAVKGSDKHSELKLNPNPASDVVEIK
                     GVDLTSEPLLKIMDATGRPLREQRLSSSTLDVSALPPGMYYLILYDSKGAFQTGRLVR
                     Q"
     assembly_gap    4302..4359
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4360..>4674)
                     /locus_tag="PK198_17860"
     CDS             complement(<4360..>4674)
                     /locus_tag="PK198_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40666.1"
                     /translation="AMNALVTVAGSFSISGNSSLVSTAGFESLTSVHGVLRVTDNPAL
                     QSLTGLDNVNYNYITWLIIANCNMLSICDVQSVCDYLELTSGPVTISNNAIGCANVSQ
                     VEA"
     assembly_gap    4675..4723
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4724..>5078)
                     /locus_tag="PK198_17865"
     CDS             complement(<4724..>5078)
                     /locus_tag="PK198_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40667.1"
                     /translation="IEISQNHALIGLNGMSSLTTVNAGLQISQNANLLDLTGLNNIQV
                     FNGHLTITSNPLLLSLSGLSNNVSSIGGALTIRANNSLTNLGGLGNITSIGGHLEISN
                     NVVLTTLSGLGPISSI"
     assembly_gap    5079..5100
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5101..>5610)
                     /locus_tag="PK198_17870"
     CDS             complement(<5101..>5610)
                     /locus_tag="PK198_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40668.1"
                     /translation="NTITPVGGNISIQTMHGLGNLSGMNGLQSVGGNLLITTNSAMVD
                     FIGLENLQIIQGTLTINGNNSLTNFNGLSGLETVNGNVLISGNPSLIDFTGLVGLNSI
                     SGNFYVQSNNSLTSMAGISSLGSVLGDVEFSLNPSLGNMQGMGQLSTIGGRLTIKQNA
                     GLTNLIGLNG"
     assembly_gap    5611..5750
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5751..5972
                     /locus_tag="PK198_17875"
     CDS             <5751..5972
                     /locus_tag="PK198_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40669.1"
                     /translation="VVDAPIIGMADRDKLLDIAKVPAIVLIDPKPERLVIALFDAILK
                     SPPTLDSAFNPFKLVSAGLLKIAKLPSIE"
     assembly_gap    6078..6105
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6106..6779)
                     /locus_tag="PK198_17880"
     CDS             complement(<6106..6779)
                     /locus_tag="PK198_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40670.1"
                     /translation="MSIFSNPLLTNLTGLSALTSIGGDVYIFGNNLLTNLNGLNALAS
                     IGGSLNVYSNPMTDLTGLNALTSVTGNVDILYMTITSLSGLNNLNSIGGNLNISFTAI
                     QNLTGLGSLTNLGGNLTLNNNGSLTNVTALAALTTIGGSLTISNNGALPSLNGLHNVV
                     AGSITSLVLTNNSLLSVCDLANICDYLSEPTNPATISGNASGCATRPEVEAACTPVVC
                     PTGDLT"
BASE COUNT         1673 a         1649 c         1667 g         1569 t
ORIGIN      
        1 ggcgtgaatt tcagcagcgc ggcccgaaat attgaggatt ggaaagcgga ggagaggggg
       61 taggtacgag atggtttttt caacattttt tttgaaattg tcgccacagt tataactttg
      121 caacaaaaaa gagcaatcag ggctgatttt gatgaaaaac agactttcgc ccgcacaacc
      181 atcattttat gagtaaagga aaagccggca tccgaccgga aggccgcacc gacgataatg
      241 ggggccagat cattggtttg caaggcatgt acgaggacta ttttctcgat tatgcctctt
      301 atgttattct cgaacgcgct gtgcctgcca tcgaagacgg gctcaaaccc gtgcagcggc
      361 gcattttgca ctccatgttt gatctcgacg acgggcgcta ccacaaagta gccaacatca
      421 tcggctatac catgcagttt cacccgcacg gcgacgccgc catcggggat gcgctggtaa
      481 acatggggca gcgcgacctg ctcatagact gccagggcaa ctggggcgat gtgcgcaccg
      541 gcgacagcgc ggcggctccc cgctacatcg aagggcggct cacaaaattt gcgctggaag
      601 taaccttcaa cccgcaaaca accgactggc aactctccta cgacgggcgc aaaaaagagc
      661 cggtcacttt gccggtcaaa tttccgctgc tgctggcaca aggcgtggag ggcattgccg
      721 tggggctttc caccaaaatc ctgccgcaca atttcatcga actcatcaaa gcctccatta
      781 aaatattgca ggggaaaaag gtcaaaattt accccgattt ccccaccggc ggctccatag
      841 acgtaagcga ttacaacagc ggcgcccgcg gcggcaaagt caaagtgcgc gccaaaattg
      901 aagtggtgga caaaagcacc ctcgccgtgc gcgacctgcc cttcggcgtc accaccggct
      961 cgctcattga gagcattgtg aaagccaacg acaaaggtaa aatcaagatt aaaaaagtgg
     1021 tggacaacac cgctcagttg gtcgaagtcc acatcgaact ggcgcccggc gtatctcccg
     1081 atgtgacggt ggatgccctc tacgccttca cagactgcga ggtctccata tcgcccaatg
     1141 cctgcgtgat tgtagaggac aagccggttt ttactacggt ggaggacatt ctggaaattt
     1201 ctaccaacaa cacccgcgaa ctgctgcgta gcgaattgga gatcaaaaag cacgaattgc
     1261 aggaaaagtg gcatttcgct tccttggaaa aaatcttcat cgaaaagcgc atttaccacg
     1321 aaattgaaga gtgcgaaagt tgggaggaag tgatggccac gatttatcgc gaactggccc
     1381 gctacgttgc cacgccttcc gacccgcccc aaccaggcga cgcccgcctt cccctgcatc
     1441 gcaacattga cgacgacgac atcacgcgcc tcaccgaaat ccgcatcaag cgcatttcta
     1501 aatacaatgc cttcaaggca gacgaactca tcgcacaaat agaggccgac ctgcggcaag
     1561 tgcaacacga tctggagcac ctcaccgagt ttgccgttgc ctattttgaa aggctgttgg
     1621 aaaaatacgg caaaggccgc gagcgccgca ccctcatcac tgagttcgac accatacagg
     1681 ctaccgaagt ggtggccaac aatgctaagc tctacgtcaa tcgcgcggag ggtttcatcg
     1741 gatccggcct gaaaaaagat gagttcgtgt gcgattgctc cgacatagac gacatcatcg
     1801 tgttccgccg cgacggcaag ttccaggtgt cgcgcatcag cgacaaagtc tttgtaggaa
     1861 aggacatcat tcatgtggcc gtttggaaaa agaacgacga gcgcaccact tacaacatgg
     1921 cctactcaga cggcaaaagc gggcgcagtt ttgtgaagcg cttcaacgtc acggcgatta
     1981 cccgcgaccg cgagtacgac ctcacgcagg gcaatcccaa ttccaaactg ctttattttt
     2041 ctgccaatcc caacggcgag gccgaaatcg tggtggtgca attgtcggcg ggcgctactg
     2101 ccaagatcaa aattttcgac tacaattttg cagaactgga aatcaaaggt cgtagctcac
     2161 aaggcaatat cctcaccaaa tatccggtca aaaaagtcac gctcaaggag gccggaaagt
     2221 ccaccctcgg cgcgcagcac gtctggattg acgaggtcag cggcaaactg aacacggaca
     2281 accggggccg cctgctgggc gatttcgata ctggcgatca gcttttcctc ctctacaaag
     2341 acggcactta cgaggtagcc gacgtggaca tgagccgccg tttcgatccc gatgaactgc
     2401 tgcatctcgg caagttcgac ccggaaatgg tgatcaacgc cgtacatttt gacggcaaca
     2461 aaggctggac gatggtcaaa cgcttccgcg tagaaaccac ctccaccggc cagcgctttt
     2521 cttatctctc ggagcacaaa gattccannn nnnnnnnnnn nnnnnttttc gcctccatca
     2581 aaaaggagcc gcgcattgag tacagcgtca agatcaaagg caaaaaaata cagggcgaag
     2641 tcaatctggc cgactttata gacgtcaaag gctggaaagc catgggcaac aaactcagcg
     2701 accagaagct cagttccgtg aaagaactcg aagacccgac gctgttccag acgcctgatt
     2761 ccaatgcagc cgaggtaccg ctgccggaga agtttcacgc cggcgacagc ttggagtttg
     2821 acatggggaa aaacgggcag ggttctttgt tttgattttc aaaactcgaa gcccggcatc
     2881 ataatgccct gcaccatcca ttcgccgtct tcatattcaa tctgactgac gccgagacct
     2941 tcgtggcgtt caggctcgcc gccatgtatc ggcgtgcctt cccaagccac cagaaagaag
     3001 aaatacatgc ccacttctgt gcgctgcacc tgctgttccc gaaaggcgat gggttttatt
     3061 tcggtgtatt tggagcgaaa gatatggatg cgctgcaata cctcggcttt ggagatggcc
     3121 tcgctttggt cgcggcaagt ctcgctgatc cattcgcctt ccacatcgcg gttgaggttg
     3181 agcaattcca gcaaagtttc ttccacatgt tcgcggatga actccggccc gtcatggtga
     3241 aaatcggaat aactgaagcc gtgaatcatg ccgggcgccg tgtagtcggt catttccgag
     3301 ttcagaaaat cctctgtcag aaagcggtag taccctctgg ggcgcaggtg ttcggggcga
     3361 tcaatgatga ctccggcggc ttccaactgc tccagcagag attggatatg cgcttccaaa
     3421 tcctcctcgg ccacctcttt gtagcccggt tgttcgggaa taccggcaaa ctcccgcacc
     3481 ggcaccatgc gcgcattggc atgagccgcc tcgaaattgg ccacattttc cagaaacatt
     3541 ttggtgattt ccggcggcaa gtcgtcggag agaaaattgg cagcgccgtg attgatttcc
     3601 agatcgagcg ctttgagctg gttctccatg cggagttcct cttcgggcgt gagtttgggt
     3661 tcttccatga tgagattggt tgagggaagc gaaggtaggg aagaatgaat atttttcccg
     3721 atttttgaac cgaattcatc caatcgcatc atggaagaaa gacttagtag tggggcgaaa
     3781 atgaccactt tattttttat cgccccttac tcatcactgc ctgacaagtc ggcccgtctg
     3841 aaaagcgccc ttgctgtcgt acaggatgag gtagtacata ccgggcggca gcgccgagac
     3901 atccagcgtg ctgctgctca ggcgttgttc gcgcaagggt ctgccggtgg cgtccatgat
     3961 tttgaggagc ggctcggaag tcaggtccac gccttttatt tcaaccacgt cagaggcggg
     4021 gttggggttt agctttaatt ccgaatgttt atcgctcccc ttcaccgcca ctatattaga
     4081 gaggcttgcc gtgccgtcga agtctatttg ccggaggcgg tagtagttgg tgccgccgag
     4141 cggttctttg tcaatcagtg tgtagtgttg agtctctgtg gtggtgccgt agccgtttac
     4201 aaatccgatc tcctgccatc ggacgccgtc gtcactgcgc tgcacttcga agccacggtt
     4261 gttggtttcg gaggcggttt gccatgaaag caagttgatt tnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcttctactt gcgagacatt
     4381 tgcacatcca attgcgttat ttgatatggt tactgggccg gatgtcaatt ctaaatagtc
     4441 acatacactt tggacatcac aaatagacag catattacag ttggctatga ttagccatgt
     4501 tatatagttg taatttacat tgtctaaccc tgtaaggctt tgcaatgctg ggttgtccgt
     4561 cacccttagt actccatgaa ctgatgtcag tgattcaaag cctgctgtac ttaccaaaga
     4621 agagttgccc gatattgaaa agctacctgc cacagtaaca agagcgttca ttgcnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaataga actaatcggg
     4741 ccgaggccag ataatgtagt caacactaca ttgttggaaa tttcaagatg tccgccaatc
     4801 gaggtaatat tgcctaaccc accgagattt gtcaaggaat tgtttgcacg tatggttagt
     4861 gcacctccta tggatgatac attgtttgaa agccctgaca agctcaacag taaagggttt
     4921 gaagttatcg ttagatgacc attaaagact tgaatattgt ttagtcctgt taaatccaac
     4981 aggttggcat tttgtgaaat ctgtaatcca gcgttaacgg ttgtaagtga actcatccca
     5041 ttcaacccaa tcaatgcatg attttgagaa atttctatnn nnnnnnnnnn nnnnnnnnnn
     5101 ccattcagac ctataagatt tgttaagccg gcattttgtt taatggtaag ccttccgcca
     5161 atggtagata attgccccat gccttgcata ttgccaagtg atgggtttaa tgaaaactcc
     5221 acatctccca atacactgcc gagcgagcta atcccagcca tgctggtcaa ggaattgttg
     5281 gattgtacat aaaaatttcc tgaaatgctg tttaatccga ccaaacctgt aaaatcaatc
     5341 aaagatgggt ttcctgaaat caacacatta ccgttgacag tttcaaggcc actgaggcca
     5401 ttaaagttcg tcaagctgtt attgccattt atagtaagtg tgccttggat tatttggagg
     5461 ttttcaaggc cgataaagtc aaccatcgct gaattggtag taattaacag gtttccaccc
     5521 acggattgaa gtccgttcat accgctcaaa ttccccaaac catgcatggt ttgaatagat
     5581 atgttcccac caacgggcgt tatggtgttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtggtagatg
     5761 cgcctattat tggaatggcg gatagggaca aattgttaga tatcgcaaaa gtgcctgcta
     5821 ttgtactaat agaccctaaa cctgaaaggc ttgtcatcgc attgtttgat gcgatattga
     5881 agtctccgcc aacacttgat agcgcattta atccatttaa attggtcagt gcaggattgt
     5941 tgaaaatagc taaattacct tcaattgaat gaattgttga aagcccattt aaattagtaa
     6001 tgtttgaacc gtttatcgaa acactaccct gaatgagggg gcaacccgga taattcgtcg
     6061 gaaaaccgtc aatctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaggt gaggtctccg
     6121 gtggggcaca ctaccggcgt gcaggcggct tccacctctg gccgagtagc gcagccggag
     6181 gcgttgccgg atatggttgc cgggttagtt ggttcggaga ggtaatcgca aatattggcc
     6241 aggtcgcaaa cagacaaaag gctgttgttg gtcagcacga gcgacgtgat ggatccggca
     6301 accacattgt gcaatccgtt cagacttggc agggcgccgt tgttgctgat ggtcaggctg
     6361 cctccgatgg tcgtgagcgc agccaaagcg gttacgttcg tcagcgagcc attgttgttc
     6421 aaagtcaggt ttccacccag attggtcaga cttcccaagc cggtcaggtt ttgaatggcc
     6481 gtgaaggaaa tgttcaggtt gcctcctatg gaattcaaat tattcagccc cgacaaactt
     6541 gtgatcgtca tataaagaat atcaacgttt cctgtcaccg acgtcaatgc gttcaagccg
     6601 gtgagatcgg tcatagggtt gctgtaaaca ttcaggctgc cgccgatact ggcaagggca
     6661 ttcaatccat tcagattggt gagcaaattg ttaccgaaga tatatacatc gccgcctatt
     6721 gaggtaaggg cgctcaggcc ggtgaggttg gtcagcaacg gattggaaaa gatgctcaag
     6781 ttgccggcaa ccgaaccgac catgttcaat gcgttcaaat tggtcagggc agcgttttca
     6841 ccaatcgaaa gccagttaac gccggtgatc ggg
//
LOCUS       DAORLF010000259         6849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_5975_length_6849_cov_2.369002, whole genome shotgun sequence.
ACCESSION   DAORLF010000259 DAORLF010000000
VERSION     DAORLF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5975_length_6849_cov_2.369002"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK198_17885"
     CDS             complement(<1..389)
                     /locus_tag="PK198_17885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF40671.1"
                     /translation="MNALADSLVIIPTYNEKENIVRMIETVLSLDAPFHLLIVDDGSP
                     DGTAALVKALQAQFPDRLFLLERSGKLGLGTAYITAFRWGLERGYEFFFEMDADFSHN
                     PNDLLRLYKACKEDGADMSVGSRYIPGG"
     gene            complement(462..1355)
                     /locus_tag="PK198_17890"
     CDS             complement(462..1355)
                     /locus_tag="PK198_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRF40672.1"
                     /translation="MNDKLTRIGVFYDGNFFLHISNYYNYEHPKQRRLSIAGLHEFIT
                     RSVAQVEGEDARRCRIVDAHYFRGRLSAHEANQRGDMLYWDRLFDDILMSAGVATHYL
                     PLKVANGRREERGIDVWLALEAFELAMRKELDVVVLIASDGDYVTLARKLHSLGTRVM
                     LLSWDFDFYADNGKQMVTRTSQDLAEEVTYPMAMHELIDEGLRNNDPVVTNIFVAGES
                     KKKVVINPEDIPDGAETSIVLSLKNGFGFIKYPPNNLFFHHTSLVNADFNDLAEGDTV
                     AFNIGTNDEGEEIAIDVQILN"
     gene            complement(1511..2035)
                     /locus_tag="PK198_17895"
     CDS             complement(1511..2035)
                     /locus_tag="PK198_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40673.1"
                     /translation="MKVSMMTWIWAAGLLLAVSSCKKGKDAPEELLQKNDKVLMMNEI
                     PAGDLAADHALLPAPGNMGSIMGTETSAGQPLRGLIIGAAAKKGVRIEIWPLGAVQWR
                     TGQELQTCIIATSPEQAAAGLEDFMALRLAHDDVRTTLEHWFRRHYIPSNAEWLGWQN
                     EVYAQAEIQRAGLR"
     gene            2199..2272
                     /locus_tag="PK198_17900"
     tRNA            2199..2272
                     /locus_tag="PK198_17900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2233..2235,aa:Pro,seq:cgg)
     gene            2334..2409
                     /locus_tag="PK198_17905"
     tRNA            2334..2409
                     /locus_tag="PK198_17905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2367..2369,aa:Thr,seq:cgt)
     gene            2540..3499
                     /locus_tag="PK198_17910"
     CDS             2540..3499
                     /locus_tag="PK198_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40674.1"
                     /translation="MHILRTSCFLGLFFITTTALSAQPDRVATGMYLMNVYDLNVSEY
                     SFYADFYLWFKWQGERDPMNIEFVNAVEKWGFTSTPFHEESLEKPDGSFYNGMRAEGR
                     FYHSFDLRRFPLDQHDLDIRIENVDYPLDELAFEAAPAKTLYRPDFVIPGWRILGARI
                     DTHSNLYDTDFGEPENSGDSFSNFSFNLRIARPLSYFLLKLMLPLLVVIVAGLGALAI
                     HPAYMDARISLPIGSLLSCVFLQQSYSSALPDVGYMVLMDKIYLLSYLLIAAIMLRVI
                     RVGNIRKRSKDEDLSRLLTKDKRVAALGFVVFLAGVTALVAWG"
     gene            complement(3505..3846)
                     /gene="hypA"
                     /locus_tag="PK198_17915"
     CDS             complement(3505..3846)
                     /gene="hypA"
                     /locus_tag="PK198_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRF40675.1"
                     /translation="MHELSIVLSIVDIAAEQVHKAGARQVDRIDLEIGLLAGIEFDAL
                     DFAWDVGVQRSVLEGAERHIHKIPARGRCMDCGHEFGIEQLYEPCPSCGQFLVEVVRG
                     KELRVKSLEVS"
     gene            3890..5173
                     /gene="kynU"
                     /locus_tag="PK198_17920"
     CDS             3890..5173
                     /gene="kynU"
                     /locus_tag="PK198_17920"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRF40676.1"
                     /translation="MKTMFDTSADCARNLDAQDTLAQLRDQFYFPQHEGRDVLYFCGN
                     SLGLQPKSVEPALLRELEHWRTHGVEGHFRGEMPWMHYHKYLQSAAAHVVGALPEEVV
                     IMNTLTANLHLLMVSFYRPDARRFKIIMEAGAFPSDQYAMESQVRYHGYAPEEAIVEI
                     APRPGEETLRTEDVLETIRQHGPQTALVMMCGVQYYTGQWMDVEAITRAGHEAGALVG
                     FDLAHAAGNIPMRLHDWQADFAVWCSYKYLNSGPGGPGGVFVHQRHADNKDLPRFAGW
                     WGHDEERRFLMEKGFVPMRGAAGWQLSNAQIFSFAAHKASLDIFESAGMGALRHKSEQ
                     LTAYLEFLIDDLTEKGLPLHIITPRDPAQRGCQLSVTAGPQGRAIFDFLSTHGVVCDW
                     REPDVIRLAPTPMYNSFGDVFALYHLLLDFLRRNQ"
     gene            5356..>6849
                     /locus_tag="PK198_17925"
     CDS             5356..>6849
                     /locus_tag="PK198_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF40677.1"
                     /translation="MSSRKNRSAGTEEEKPKLSKDKLRRALKLYSYVLPHKWAFIAGM
                     VFLSLGSAIFLAIMKLPGAILDAVNGSHSNGMSIERIVSLLFILLGIQALFSYIRVRL
                     FAVVSERSMAALRNDLYAKLITLDIPFFEERRVGELTSRITNDVTQVQGVISITLAEF
                     IRQIIIFIGGVVIIVVTMKTLALILLATVPIVMVLAMVFGRYVRKLMKARQDQLAEAN
                     VIVEETMQSIQSVKAYTNEGFELGRYSRQVNTTVNTSLQAAHMRGLFAAFIIFVMFGA
                     LFFIIWRAAIMVQSGVLTEGSLIDFTVYAAIIGGAIASLGSFYTEIVSAVGATDRILD
                     ILEQPSEVAVAPAETQAHSRFEGKVEFQEVHFSYPTRKDVEVLKGINLKIQPGQRIAL
                     AGPSGAGKSTIIQLLLQFYKPDSGTILVDERPANEYDLRAYRQNMAIVPQEVMLFGGT
                     IRDNILYGKPGATDEEVIEAARQANAWDFISAFPDGLDTIVGERGVKL"
BASE COUNT         1496 a         1952 c         1794 g         1607 t
ORIGIN      
        1 ccgccgggta tgtagcgcga tcctaccgac atatcggcgc cgtcttcttt gcaagccttg
       61 tacagccgca acaaatcgtt gggattgtgc gagaagtcgg catccatttc aaaaaagaac
      121 tcgtagccgc gctccaagcc ccaacgaaaa gcagtgatgt atgccgttcc cagccccaat
      181 ttgccggatc gttccagcag gaacaggcgg tcgggaaact gcgcttgcag ggctttcacc
      241 aaagcggcag taccgtcggg cgatccgtcg tccacgatca gcaagtgaaa aggcgcgtca
      301 aggctcaaca ccgtttcgat catgcgaacg atgttttcct tttcattgta ggtaggaatg
      361 attactaagc tgtctgccaa ggcgttcaaa ggtaagagtg gcgctgattg cgcgttcaaa
      421 gatacgataa ctaacgcaaa aacgaccggt gagcgtgctt gttaattcag tatttgcaca
      481 tcaatggcaa tctcttcgcc ctcatcgttg gtaccgatgt tgaatgccac cgtgtctcct
      541 tctgccaggt cgttgaaatc tgcattgacc aagctggtat gatggaaaaa caggttgttc
      601 ggcgggtatt tgatgaagcc gaagccgttt ttcagactga gaacgatgct ggtttctgcc
      661 ccgtcgggaa tatcttccgg attgatgacg acctttttct tgctctctcc ggccacaaag
      721 atattggtca ctaccggatc gttgttgcgc aaaccttcgt ctatcagttc gtgcatggcc
      781 atcgggtagg tcacttcttc ggctaagtct tgcgaagtac gggtcaccat ttgcttgccg
      841 ttgtcggcgt aaaagtcgaa atcccaactt aggagcatca cgcgggtgcc aagcgagtgc
      901 agcttgcgcg ccagcgtcac atagtcgccg tcggaggcaa tgagcaccac tacgtccaac
      961 tctttgcgca tagcgagttc gaaggcttcc agtgccagcc acacgtctat gccgcgctct
     1021 tcgcgacggc cgttggccac tttgagcggc aaatagtggg tggccacgcc tgccgacatg
     1081 aggatgtcgt caaacaggcg gtcccaatac agcatatcgc cgcgttgatt ggcttcgtga
     1141 gcgctcaggc ggccccggaa atagtgcgca tccacaatgc ggcaacgcct tgcatcctct
     1201 ccttccacct gcgccacact gcgcgtaatg aactcgtgaa ggccggcaat gctgagcctg
     1261 cgctgcttcg ggtgttcata attgtaataa ttgctgatgt gcaggaaaaa attaccgtcg
     1321 tagaatacgc cgatacgtgt cagtttgtca ttcataatgt cgagctaaat aagatataga
     1381 tttaaaaata gggctgcaaa gatacgtttc tcgaagaatt gtgcaaacgc gaagccacaa
     1441 agacgccccg atttatacca atccgctccg ctcgtccacc aaaaatgctc ctcgttcgcc
     1501 gggcacctat tcaacgcagg cccgcacgtt gtatttcagc ctgggcgtac acctcgtttt
     1561 gccaaccaag ccactcagca ttggagggta tgtagtggcg ccggaaccag tgttccagcg
     1621 tagtgcgaac atcgtcgtgg gccaggcgca gcgccataaa atcctccaga cctgccgccg
     1681 cctgttcagg cgaggtcgca atgatgcagg tttgcaactc ctgccctgtg cgccattgca
     1741 ccgcgcccaa cggccatatt tctattcgta ctcctttttt tgcagccgcc ccgataatga
     1801 gaccgcgcag gggttgcccc gcagaggttt ctgtccccat gatgcttccc atgtttcccg
     1861 gcgcgggcaa cagcgcatga tcggcagcca ggtctcctgc gggtatttca ttcatcatca
     1921 acactttatc gtttttttgc aacagttcct ccggtgcatc cttgcctttt ttgcatgacg
     1981 ataccgccag caacaagcca gctgcccaaa tccaggtcat catcgaaacc ttcatacgca
     2041 ttccttcatt tgtcgccaca aagtaggcac tatggcgtgc ttttagggta tgcgggttca
     2101 ttttggaaaa aaaattatgc gcaggggttg actaaaacgc acaatcatct tacctttgcg
     2161 cctctctaat ggaaacgtcc atttttaaga gcgcttttcg gggtgtagcg cagcttggta
     2221 gcgcgcgtcg ttcgggacga cgaggtcgga ggttcgaatc ctctcacccc gaccgaaaag
     2281 tctgactttc aggcaaaaac atcagatgca ggcaactgtt tttcacctgt gttgccaccc
     2341 tagctcagtt ggtagagcaa cgcattcgta atgcgtgggt cgggaggtcg agtctcctgg
     2401 gtggctccaa taagtttcaa gttcaaaagc cttcggcgtg ttgcgtcgga ggcttttgcg
     2461 tttgtggccc ttcgcaaccg gagagggttg atcgtttgaa aaaataaggc tacttttgtc
     2521 tgcgattaag aactgacgca tgcacattct ccgcacctct tgttttttgg ggcttttctt
     2581 tataacaaca accgccctct ccgcccaacc cgaccgcgtt gctaccggca tgtacctcat
     2641 gaacgtatat gacctcaatg tgagcgagta ctcgttttat gccgatttct acctgtggtt
     2701 caaatggcag ggcgaacgcg accccatgaa catagagttt gtcaacgccg tcgaaaaatg
     2761 gggcttcact tccacgccgt ttcatgaaga atcactggaa aaaccagacg gcagtttcta
     2821 caacggcatg cgcgctgagg ggcgttttta tcactccttc gacctgcgcc ggttcccttt
     2881 agaccagcac gatctcgaca tccgcatcga aaatgtggac taccccttag atgaactcgc
     2941 cttcgaagcc gcccccgcca aaacgctgta tcgccccgat tttgtcatcc ccggctggcg
     3001 catcctcggt gcgcgcatag acacccactc caatctatac gacaccgatt tcggtgagcc
     3061 ggaaaacagc ggcgattcgt tcagcaattt cagcttcaac ctccgcatcg cgcgccccct
     3121 gagctatttc ctgctcaaac tcatgctccc gcttttggtg gtcatcgtgg ccggattagg
     3181 cgctctggcc attcacccgg cttacatgga tgcgcgcatt tccttgccca tcggcagttt
     3241 gctgagttgc gtttttttac aacaatctta cagctcggcc ctgcccgacg taggctacat
     3301 ggtgctgatg gacaaaattt atctgctttc ctacctgctc attgcggcca tcatgttgcg
     3361 cgttatccgg gtgggcaata tccgcaaacg cagcaaagac gaggatttat cgcgcctgct
     3421 gaccaaagat aaacgtgtcg cggcgctggg gttcgtggtt tttctggcgg gcgtgactgc
     3481 attagtggcc tggggttaaa catatcaact tacctccaac gacttcaccc gcaactcctt
     3541 gccccttacc acctccacca gaaattggcc gcaggagggg caaggctcgt acaattgttc
     3601 tatcccgaac tcgtggccgc agtccatgca gcgcccccgc gccgggattt tgtgaatgtg
     3661 ccgttcggcg ccttccaata cgctgcgctg cacgcccacg tcccaggcaa agtccaaagc
     3721 gtcaaattca atgccggcca aaagcccgat ttccagatca atacgatcca cttggcgggc
     3781 gccggccttg tgtacctgtt cggcggcgat gtcaacgatg ctgagtacaa tagagagttc
     3841 gtgcatgatt tttagcggtt gcgccacaaa tatagcctaa ttttacggca tgaaaacgat
     3901 gttcgatacc agcgccgatt gcgcccgaaa cttagacgct caggacaccc tggcgcaact
     3961 gcgggatcaa ttctattttc cgcaacatga aggcagggac gtgctttatt tttgtggcaa
     4021 ttcattggga ttgcaaccca aatcggtgga gccggcgctg ctgcgcgagt tggaacactg
     4081 gcgcacgcac ggcgtagagg gccattttcg cggcgagatg ccctggatgc actaccacaa
     4141 atatttgcaa tcggcagcag cgcatgtagt cggcgcattg cctgaagagg tggtgattat
     4201 gaacaccctg acggccaacc tgcacctgct catggtgtcg ttttacaggc ccgatgcccg
     4261 gcgcttcaag atcatcatgg aggcgggggc ttttccttcc gatcagtacg cgatggaaag
     4321 ccaggtgcgc tatcacgggt acgcgcccga agaggccatc gtggaaattg cgccccgacc
     4381 cggcgaggaa accctgcgga cagaggatgt tttggaaacc atccggcagc acggcccgca
     4441 aacggctttg gtgatgatgt gcggcgtgca atattacacc ggccagtgga tggatgtgga
     4501 ggccatcacc cgcgcagggc atgaagcagg cgctttggtg ggcttcgatc tggcgcacgc
     4561 tgcgggcaac ataccgatgc gcctgcacga ttggcaggcc gactttgccg tgtggtgcag
     4621 ctacaaatat ctcaattctg gccccggcgg ccccggcggc gtttttgtac accagcgcca
     4681 tgccgacaat aaagacctgc cgcgctttgc gggttggtgg ggccacgacg aagagcgccg
     4741 cttcctcatg gagaaaggat ttgtgcctat gcgcggagca gcgggctggc aactgagcaa
     4801 tgcccagatt ttctcctttg ccgcgcacaa ggcttcgttg gacatatttg aatcggcagg
     4861 catgggcgcg ctgcggcaca agagcgaaca actgaccgcc tacctcgaat ttctcataga
     4921 tgatttgacc gaaaaagggc tgccgcttca catcatcacc ccccgcgatc cggcgcaacg
     4981 cggttgccag ctctcggtga cggcagggcc gcagggcaga gccattttcg actttttgtc
     5041 cactcatgga gttgtttgcg attggcgcga accggatgtc attcgcctgg cgcccacgcc
     5101 catgtacaat tctttcggcg atgtgtttgc actgtaccat ctgctgttgg atttcctgcg
     5161 ccgcaaccaa tagctgggcg cctgggttat atacctcgca ccgtcaggtg caaagccgct
     5221 tttatcatac ctggcgctgc taatcaataa cttacgccga aggctataca caaaatctgg
     5281 cggcgtaagg tataacatcc tccattctcc gggcaggcgc ttgtcgtcgg tccgattcaa
     5341 aatttaaaac gatccatgtc ttcccgaaaa aatcgttctg ccggaaccga agaagaaaaa
     5401 cccaaactca gcaaagacaa actgcgccgg gcgctgaaac tgtacagcta cgttttgccg
     5461 cacaagtggg cattcattgc cggcatggta tttttgtcat tgggcagcgc catttttctg
     5521 gccattatga aattgccggg cgctatctta gacgctgtca acgggagcca ttccaacggc
     5581 atgtccattg agcgcatcgt ctccttgctc ttcattttac tgggcattca ggcgctgttt
     5641 tcctacattc gggtgcggct ttttgctgtc gtcagcgagc gcagcatggc cgccctgcgc
     5701 aacgacctgt acgccaagct catcacgttg gacataccat tttttgaaga gcgccgcgtg
     5761 ggcgaactca ccagccgcat caccaacgac gtcacacagg tgcagggcgt catctccatt
     5821 acgctggccg aattcatccg gcaaatcatc atcttcatcg gcggagtcgt catcattgtg
     5881 gtcaccatga aaactctggc gctcatcctt ttggccaccg tacccattgt gatggtgctg
     5941 gcgatggttt tcgggcgcta tgtgcgcaaa ctgatgaaag cccggcaaga ccaattggcc
     6001 gaagccaacg tcattgtgga ggaaaccatg cagagcatac agtcggtaaa agcctatacc
     6061 aacgagggct tcgaactggg ccgctattcc cggcaggtga acaccaccgt caacacctcg
     6121 cttcaggctg cgcacatgcg cggtttgttt gcggcattca tcattttcgt catgttcggg
     6181 gccttgtttt tcatcatctg gcgggcggcc atcatggtgc agtcgggcgt gctcaccgag
     6241 ggcagcctga ttgatttcac cgtgtatgcg gccatcatcg ggggcgccat tgccagtttg
     6301 ggcagttttt acacagaaat tgtatcagct gtcggcgcca cagaccgcat tttggacatt
     6361 ctggagcagc catccgaagt ggccgtcgca cccgctgaaa cccaagccca cagcaggttt
     6421 gaggggaaag tcgaatttca ggaggttcat ttttcctacc ctacccgcaa agacgtggag
     6481 gtgctcaagg gcatcaacct gaaaattcaa cccggccaac gcattgcact ggcaggcccc
     6541 agcggcgcag gcaaatccac catcattcag ttgttgctcc aattttataa acccgacagc
     6601 ggcacgatct tagtggacga gcgcccggcc aacgaatacg acctgcgcgc ctaccgccaa
     6661 aacatggcca ttgtaccgca ggaggtgatg ctgtttggcg gcaccatccg cgacaatatc
     6721 ctgtacggca agcccggcgc cacagacgag gaagtgatcg aagccgcccg ccaggccaat
     6781 gcctgggatt tcatctccgc gttccccgac ggcttggata ccatcgtggg cgagcgcggc
     6841 gtgaaactc
//
LOCUS       DAORLF010000260         6806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6031_length_6806_cov_2.275515, whole genome shotgun sequence.
ACCESSION   DAORLF010000260 DAORLF010000000
VERSION     DAORLF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6031_length_6806_cov_2.275515"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1043)
                     /gene="aroC"
                     /locus_tag="PK198_17930"
     CDS             complement(125..>1043)
                     /gene="aroC"
                     /locus_tag="PK198_17930"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRF40678.1"
                     /translation="IVTQRKEEDQAHILSGVFEGRSTGMPIAIVIYNADARSKDYSHI
                     AESFRPSHADYTYHAKYGFRDYRGGGRSSARETAARVAAGAVAKMLLATLGVQVNAWV
                     SQVGDIVLEKPYSELDLRLTESNPVRCPDPDTAARMEALIREVRKDGDTIGGVVNAVA
                     TGVPAGWGEPVFDRLHADLGKAILSINACKGFEYGSGFAGAAMRGSVHNDAFVQQDGR
                     IVTATNHSGGIQGGISNGMDIYFRAAFKPVATIVPAQKSVDVHGEPVTVEGKGRHDPC
                     VLPRAVPIVEAMTAIVLADHYLRSWSSRV"
     assembly_gap    1044..1053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1054..1240)
                     /locus_tag="PK198_17935"
     CDS             complement(<1054..1240)
                     /locus_tag="PK198_17935"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRF40679.1"
                     /translation="MPGNTFGNTFKISTFGESHGPAIGVVIDGCPAGLAIDEQFLQSE
                     LDRRRPGASGARSRHRRH"
     assembly_gap    1285..1322
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1683..2027
                     /locus_tag="PK198_17940"
     CDS             1683..2027
                     /locus_tag="PK198_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40680.1"
                     /translation="MENSGNPISSGGTDPKIVGIVSYLTLIGWIVALVLNNPKSEHGS
                     FHVRQALGILLTAVISGFIAIIPILGWIVALVGYVLAFVMWIMGFIGAVQGEQKVVPV
                     LGDKFQEWFKSL"
     gene            complement(2090..2881)
                     /locus_tag="PK198_17945"
     CDS             complement(2090..2881)
                     /locus_tag="PK198_17945"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRF40681.1"
                     /translation="MDQKLMLSDLFHIALLIFAYANVWFLISLALRRNDVADIAWGLG
                     YLLIAGYFIATRPLSETAWWVYGASAVWALRLAGHIFLRTLRKKEDFRYRQWRQDWGR
                     SFYLRSWLQVYMLQAAILLVVAAPLIVAGWVPQGESGFWTGFGMGVWTAGFLLETVAD
                     HQLARFLKTRKNSDEILQTGIWKNIRHPNYSGEIIVWWGMFFMVAALPYGWAALISPV
                     VITLLLTRVSGVPMLEKRYEGNTDYEAYKKRAGALWPKPSERWSQ"
     gene            complement(2866..3276)
                     /locus_tag="PK198_17950"
     CDS             complement(2866..3276)
                     /locus_tag="PK198_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRF40682.1"
                     /translation="MPFLQLLFSYLLTALVFFAIDLVWLGLIAKGMYNRHLGHLLSEQ
                     VNWAAAIVFYLLFIVGIFIFGILPAVEKESWRYALLYGALFGFFTYATYDLTNLATLK
                     NWPVQIILPDLLWGMALSGATAVAGYFITQWIKN"
     assembly_gap    4082..4118
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4119..4686
                     /locus_tag="PK198_17955"
     CDS             <4119..4686
                     /locus_tag="PK198_17955"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRF40683.1"
                     /translation="ALLRLIPGLADKGWAEKTARIASAVVILALGLVVFKQLVMALAA
                     PIMSPLSEKVERYLAGDTYHEIRFSAGQVISDMVRGLRIALRNVVRELIFTVLLLLLG
                     LIPVIGLAAPVLIFVVQAYYAGFGNMDYTLERHFGVRESVRFVRRHRGAAIGNGVVFL
                     LLLLTGVGFLFALPLGAVAATVETRKRL"
     gene            4939..5928
                     /locus_tag="PK198_17960"
     CDS             4939..5928
                     /locus_tag="PK198_17960"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRF40684.1"
                     /translation="MSILPHWFRHYTGVLRGWKPAYMLHNILNARHLLQNRVVYQRYG
                     LKKSIFSTIGSKDFQQRSPHIPWLDQPGAKERLIKDPQFHQFPLAWQKELLRFVNDGY
                     MILQGFFSPDAVGRLNADIERLLSDKQLDFNYTGRKIMESYRHSEVADKEFFRQPDLL
                     QLLTFIMGRKVKPFHTINFVEGSEQRAHSDSIHMSTEPQGYLIAAWAALEPTGADNGP
                     LFFYPGSHRLPYVTCEDYPSGNTRWTIGADSNRRYEDKIAEILSEHSFERREFYAQPG
                     DVLIWHANLIHGGSPILRPGATRRSMVAHYFCEGVICYHEISQRPAIAPSLNS"
     gene            5932..>6409
                     /locus_tag="PK198_17965"
     CDS             5932..>6409
                     /locus_tag="PK198_17965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF40685.1"
                     /translation="MSMLRSLYYALPPAMRFRARRLWYAPLDLWESISGKRDALTPPR
                     GLIFTGGGDFRAQGDRMAEVFRRHAGLLPRHRVLDIGSGIGRIAVPLTGILDASGSYE
                     GFDVVEMGVRWCQKRISTRFPRFQFRYVPLANDLYRADGQEADQFRFPYADADFEFA"
     assembly_gap    6410..6439
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6440..6784
                     /locus_tag="PK198_17970"
     CDS             <6440..6784
                     /locus_tag="PK198_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40686.1"
                     /translation="THFLPDEVEQYLRETSRVLKPGGTFCATFFLLNAASEALLPGRT
                     QFAFPHDHGHYRLMDERVRAANVSFREDWLRALAQGCGLGIEAIHYGYWCGREKGECI
                     DFQDVVVFRRMK"
BASE COUNT         1393 a         1845 c         1975 g         1478 t
ORIGIN      
        1 gagctgttag ctttatctcc gcaatggccc gcccaagcca acagctctat ccccgctgcg
       61 ggcgccgggt gttccgctct tatagcctcc taaggtcggc tataccgctt caccccctgc
      121 tgcgtcatac cctgctgctc caacttctga ggtagtgatc ggccagcaca atggccgtca
      181 tagcctccac aatgggtacg gcgcggggca atacgcaggg gtcgtgccgc cccttgccct
      241 ctacggtcac cggttcgcca tgcacatcca ccgatttttg ggccggcacg atggtggcta
      301 cgggcttgaa cgccgcccgg aagtagatgt ccatgccgtt ggaaatgccg ccctgaatgc
      361 cgcccgaatg attggtagcg gtcacaatcc ggccatcctg ctgcacaaaa gcatcgttgt
      421 ggacagagcc gcgcatggcc gcccctgcaa aaccggagcc gtactcgaag cccttgcagg
      481 cattgatgct gaggatggcc ttgccgaggt cggcgtgcag acggtcgaaa accggttcgc
      541 cccagcctgc cggcacgccc gtcgccacag cattgaccac cccgccgatg gtgtcgccgt
      601 ctttgcgcac ctcgcggatg agcgcctcca tgcgggccgc cgtgtcgggg tcggggcagc
      661 gcacggggtt gctttcggtg aggcgcagat cgagttcgga ataaggtttt tccagcacaa
      721 tatcgcccac ctgacttacc caggcattta cctgtacgcc caaagttgcc agcaacatct
      781 tggccacagc gccggccgcc acccgtgccg ccgtttcgcg ggccgaggag cgaccgccgc
      841 cccggtagtc gcggaaaccg tacttggcgt ggtaggtata gtcggcatgc gaggggcgaa
      901 acgattctgc aatgtggctg tagtctttcg agcgggcgtc ggcattgtaa atgacgatgg
      961 cgatgggcat acccgtgctg cgcccctcaa aaacacccga caaaatatgc gcctgatcct
     1021 cctcctttcg ctgggtgacg atgnnnnnnn nnnagtggcg ccgatgacga gagcgggcgc
     1081 cactggcgcc tggccgccgc cggtcgagtt ccgattgcaa aaactgctca tctatggcca
     1141 agcctgccgg gcatccgtct ataactaccc ctatggccgg gccgtgcgat tcgccgaaag
     1201 tactgatttt aaatgtgttg ccgaatgtat tgcctggcat tgttcacgaa ggtttttttg
     1261 attcaatgtt caaggtttcg ggttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nncctcaagc tgcaagccgc gggttcctct cgctcctcgc cacgacgaca ggcgtggttc
     1381 gcggaatttc gcgcccttcg ctaagattcg cgttaaaaaa tttcgcgttt cgggttcaag
     1441 gtttcgggtt taccccgacg ataaaccgca caccgcgcca caggcgcggc ccctctccgt
     1501 tggccgataa accgccgaag gcagattacc tacccgttcc caccacgacg ataaactgcc
     1561 aaaggcagat ttcccctttc atctgcaaaa gtgacctttt cttctaattt tcagcagtat
     1621 tatgtggtgc ggattcaaaa aacgcctaca tttgatgtat tgatcaccaa aacttgaaaa
     1681 caatggaaaa ttccggaaat ccgatttcat ccggcggaac cgatcccaaa attgtgggca
     1741 tcgtatctta cctcaccctt atcggctgga ttgtagcgct cgttctcaac aatcctaaaa
     1801 gcgaacacgg ctcgtttcat gtacggcagg cgcttggcat tttgctgact gcggtcattt
     1861 ccggcttcat cgccatcata cccattctgg gttggattgt agcgctcgta ggctacgttc
     1921 ttgcttttgt gatgtggatt atgggcttca ttggcgctgt acagggcgag caaaaagtag
     1981 tgccggtatt gggcgataag tttcaggagt ggttcaaatc gctgtaaaag caagccaatc
     2041 ctcttctctg ccaacagcca tcccgatatt ttcggggtgg ctgtttttgt tattgcgacc
     2101 atctttctga aggcttcggc cacagcgcgc ctgctctttt tttatacgcc tcataatcgg
     2161 tgttgccttc ataccgcttt tccagcatgg gcacgcccga aacgcgggtc aacagcaacg
     2221 tgatgacaac gggactaatg agggctgccc agccatacgg cagcgctgcc accataaaaa
     2281 acatgcccca ccacactatg atttcgccgc tgtaattggg gtgccggatg tttttccata
     2341 tgccggtttg aagaatttca tccgaatttt tgcgggtttt caggaagcgg gccaattggt
     2401 ggtccgctac ggtttccaat aaaaaaccag ccgtccagac gcccataccg aagcccgtcc
     2461 agaagccgga ttcgccctgc ggcacccagc cggcaacgat gagcggcgcc gcgactacca
     2521 acaaaatagc ggcttgcagc atatagacct gcaaccagga acgcaggtaa aaactacgcc
     2581 cccagtcttg ccgccactgc cggtagcgga agtcttcttt tttgcgaagg gtgcgcagaa
     2641 agatatgccc ggccagccgc aaggcccata cagccgaagc gccatacacc caccaagcgg
     2701 tttcggacag cgggcgcgtg gcgatgaagt accccgcgat gagcagatat cccaggcccc
     2761 aggcgatgtc ggccacgtcg ttgcggcgga gggccagcga gatgaggaac cagacattgg
     2821 cgtaagcgaa aatcagcagg gcgatgtgaa acaggtccga cagcatcagt ttttgatcca
     2881 ttgggtgatg aaatagccgg ctacggctgt ggctccggag agtgccatgc cccagagcag
     2941 gtcgggcagg atgatttgca cgggccagtt tttgagggtg gccaggttgg tgagatcgta
     3001 ggtggcgtag gtgaagaagc cgaaaagcgc gccgtagagc agggcgtagc gccaggattc
     3061 tttttcgacg gcgggcagga tgccgaaaat gaaaatgccg acgatgaaaa ggaggtaaaa
     3121 cacaatggct gcggcccagt tgacttgctc gctgagcagg tggccgaggt ggcgattgta
     3181 catgccttta gcgatgaggc cgagccagac gaggtctatg gcaaagaaaa cgagggcggt
     3241 gagcaggtag ctgaacagga gttgaaggaa cggcatcgta tgtgttttga gagtgtaaca
     3301 aagataatgg gaaaagttca tgcgctgcac acggaagtgg gactttgcgt gggcgggtgg
     3361 aacccgcagg atagttggca cgaggtagaa cctcgcgcga gcggtgtgat tacccgggcc
     3421 atcgccgtgc aaatcatccc ttcactgcct atgtacggtc ggctttacgg ttacgaatcc
     3481 tggaaggctt cgcaaccgcc tgcatacgag tgagagctgt tagctctgcg ggcggaaaat
     3541 ttaacacata cactgcgcac taaaaagcgt ggccgggtgt tattgataca gacgtttgca
     3601 tcatgcgtct gttttcgttt cttaaccagt gagtgtaagg caacggctcc cggcaggatt
     3661 gcggggagcc gttctttttt ttggagggcg cggatgtgtc gcggtgcgcc aggggcagga
     3721 gcggtatgcc gcagaggcca aagggcaaaa agcatagagg ccctgcggta tgagagcgga
     3781 tgacccgacc cgccatttga ttgccctgca tttaccagac tgcgggcggc gggtggcgcc
     3841 ataaaaaatc atcttggtcg aaagattgcg aaaagtggta gagcgaggtg tgcggcgtgc
     3901 ggccggggct gcgtatcttg cggcaaaatt tccttgatat gaaaagcatt ttggacggaa
     3961 tagcctcgta tggcaaggcc atctcgctgg taaatcgcct cggcttgtgg ggctatgtgc
     4021 tggcgccggg cttggtgagc attgtagtgg cggtgggctt gtttggagcg gcctggggct
     4081 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cgctactgcg gctgataccg
     4141 ggcctggccg acaaaggctg ggcggaaaaa acggcccgga ttgcaagcgc ggtggtgatt
     4201 ttggcgctgg gattagtggt gttcaaacaa ttggtgatgg cgctggcagc gccgatcatg
     4261 tcgccgctgt cggaaaaagt ggagcgctac cttgccggcg atacctacca cgagattcgc
     4321 ttcagcgcag gccaggtgat ttcggacatg gttcggggct tgcgcatcgc gttgcgcaat
     4381 gtggtgcgcg agttgatatt cacggtttta ctcttgttgc tggggcttat tccggtcatc
     4441 gggctggcgg cgccggtgct gatttttgtg gtgcaggcgt actatgcggg cttcgggaat
     4501 atggactata cgctggagcg gcatttcggg gtgcgcgaga gtgtgcgttt tgtgcggcga
     4561 caccggggcg cggccatcgg caacggggtg gtgtttttgc tgctgttgct cacgggggtg
     4621 ggttttcttt ttgcgctgcc tttgggggct gttgcggcta cggtggagac gaggaagcgg
     4681 ctgtaggcgg gagtggcggc aagccataag cagcgagctg caagtttgat ttttccgacc
     4741 caaaagaagc tattttcaaa atcacatttc gccatttccc caagtggaaa atctgatttt
     4801 tcacaacagt acagacttgc agttcgcaac tcgtggctcg cagcttttgg aaggcgtaca
     4861 aaatagttgg aaaaattccg acatttgtac tttcatcttc tacgtcacca aatgcggcgc
     4921 agttgcgcca aaaacacgat gagcatcctc ccacattggt tcagacatta taccggcgtg
     4981 ctgcgcggct ggaaacccgc ctacatgctg cacaacatcc tcaatgcgcg gcacctgctg
     5041 caaaaccggg tggtgtatca gcgttatggc ctgaaaaaga gcattttcag taccatcggc
     5101 agcaaagact tccaacagcg cagcccgcat attccctggc tcgatcagcc cggcgccaaa
     5161 gaacgcctca tcaaagaccc gcagtttcat cagtttcctt tggcctggca gaaggagttg
     5221 ctgcgttttg tcaacgacgg ctacatgatt cttcagggct ttttcagccc ggatgccgtc
     5281 ggcaggttga atgccgacat agagcgcctg ttgagtgata agcagttgga tttcaactac
     5341 acgggccgga aaatcatgga atcttaccgg cacagcgagg tggccgacaa ggaatttttc
     5401 cgccagcccg atttactgca actcctcact ttcatcatgg gccgcaaggt gaaacctttc
     5461 cacaccatca atttcgtgga gggtagcgag caacgcgcac attccgactc catccacatg
     5521 agcaccgagc cacagggcta cctcatagcc gcctgggcgg cgctggaacc caccggcgcc
     5581 gacaacgggc cgttattttt ttatcccggc agccaccgcc tgccctatgt cacctgcgag
     5641 gactatccat cgggcaatac ccgctggacc atcggcgccg acagcaaccg ccgctatgag
     5701 gacaaaatag cggagatact gtcggagcac agcttcgagc gcagggagtt ctacgctcag
     5761 cccggcgatg tgctcatctg gcacgccaat ctcattcacg gcggcagccc catacttcgc
     5821 cccggcgcta cgcggcgcag catggtggcc cactactttt gcgagggcgt gatctgctac
     5881 cacgaaatct cgcaacgccc cgccattgcg ccatccctca attcctgagc catgtccatg
     5941 ctccgcagtt tgtattacgc acttccgccg gccatgcgtt tccgggcgcg gcggctctgg
     6001 tatgccccgc tcgatttgtg ggaaagcatc agcgggaaac gcgacgccct cacgccgcct
     6061 cgcggactca ttttcaccgg cggcggcgac ttccgggcgc agggcgacag aatggcggag
     6121 gtgtttcgcc gtcacgccgg gttgctgccc cggcatcgcg tgctcgacat aggaagcggc
     6181 atcgggcgca tagcggtacc gctcaccgga atactcgatg cgtcaggcag ttacgaggga
     6241 ttcgatgtgg tagaaatggg cgtgcgctgg tgccaaaaac gcatcagcac ccgctttccc
     6301 aggtttcagt tcagatatgt gcctttggcc aacgacctct accgggcaga cgggcaggag
     6361 gccgaccaat tccggtttcc ctacgctgat gcggatttcg aatttgcctn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnna cccatttttt gcccgatgag gtggagcaat acctccgcga
     6481 aacgagtcga gtgctgaaac ccggcggcac tttttgcgct actttttttc tgctcaatgc
     6541 ggcttcggag gcgctcctgc ccgggcgcac ccaatttgcg ttcccgcacg atcacggcca
     6601 ttaccggctg atggacgagc gggtacgggc ggccaatgtg tcgttccgcg aggactggct
     6661 gcgggccttg gcacaaggct gcggtctggg cattgaggcc attcactacg gctactggtg
     6721 tggcagagag aagggggaat gtattgattt tcaggatgta gtggtgttta ggagaatgaa
     6781 ataggatgca tcgcttacgg cttttt
//
LOCUS       DAORLF010000261         6791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6050_length_6791_cov_2.070071, whole genome shotgun sequence.
ACCESSION   DAORLF010000261 DAORLF010000000
VERSION     DAORLF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_6791_cov_2.070071"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3138
                     /locus_tag="PK198_17975"
     CDS             <1..3138
                     /locus_tag="PK198_17975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40687.1"
                     /translation="VPINTPVIATPANITNVNCFGASTGAIDISVSGGTGNYTYMWSY
                     QNRTTQDLTNIPAGTYSVTVTDTGTNQTTSGSFTVTQPVSGISIIAIPTNVACTGAST
                     GVITLNVSGGTGSYNYNWSGTLPDGVPSQSNLAAGTYSVTVTDGNNCTASQSVTITEP
                     AGTPLTISSMVQPVVCGGASTGRITLTISGGTPNYSINWCCGLPANATTVSNLTAGTY
                     NVTVTDNNGCTATRSINVGENPVLQISTITPTHINNGNDGAINITVTGGTGGYSFVWT
                     GPAGFNGSNQPNLSGLNFPGQYCVTVTDNSGCTASRCVQLDERLRFGNVVIGRSCAGA
                     SSGSIQVSVLGGVGPYNYNWNPGGQAGSNRTNLAAGTYNVTVTDQVGGVLAGSFVVEN
                     FPTAVVSGTVVNASGNITDANGSVTLNISGGTPGYTINWAPGGTTGLVLSNVVTGQYC
                     ATVTDMAGCSVVQCFTVGFNPGPLAIVNTQTTPVTCPGDQNGALNITLNGGLPPFTFA
                     FSDGFNQTINGVTLNRSNLPAGALTYTVTDAQGTVINGSATITASSVVTTTNIAVVHD
                     SEEPGCTGSVTLTLTGGTLPYQLQWNTPNTGGPAINNLCAGTFIATVTDGNGCIFVLP
                     GIEVNTFGLSSTSTATNCPGDATGAINLVVTGGRAPYTYEWRNAAGAIVSTDEDLANA
                     APGTYTVRVTEGSGNVLTRTYTIASQSALALDVDVTSNYNGFDVRCHNSTDGSMAATA
                     FNGQGNYTFEWRRNGTVVGGSGATLTNAAPGEYLVRVTDGLGCTVTETLTLQAPQPVT
                     VTANTLPTSCTGVNDGQIFAAASGGVFALPYNYEWSNGAFGPRLTFLRVGSYTVTVND
                     VNNCTATATFSVSNPAPIVVTVNSTPATDGCNGSVLAVVTGGGEGPFTFTWSTGQVTA
                     EPFLTGLCPGEYFVMVTDSRGCRPDPNLASGTVRDRRTPCLDIRTVITPDGDGLNEEF
                     IISCVDEFANNHLEVYNRWGQLVYETDNYDNTWRGTTRNGSELPDGPYYFVFEYTDID
                     GIRQQLRGSITILRR"
     gene            3232..>3648
                     /locus_tag="PK198_17980"
     CDS             3232..>3648
                     /locus_tag="PK198_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF40688.1"
                     /translation="MKKILLLSIIAVAALMQVQAQDQAIFSHYNINPILIHPAAAGFS
                     GQHQLLFNARMQWTGFPDAPKTFMAQYNGPLSKSFGIGFGVLSESAAQLQRTRAQLNY
                     AIRFPLGKDWEMAAGFSTELQQIRLHGDVAGGSFVDL"
     assembly_gap    3649..3675
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3676..4154
                     /locus_tag="PK198_17985"
     CDS             <3676..4154
                     /locus_tag="PK198_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF40689.1"
                     /translation="DANMSGVRFLDASVGIYGTYKENTFGGLSFTNLVRSRLDGLVVS
                     DEKESLFNYYVFYLGHKFEVEDLKFTLEPSVMARQIRDVPFQLDINLKAGFLDDQLLA
                     GVSYRSLGVMGVMLGVQVSPFNLYYTYDVSFQRFQQFNSGAHEVTIALNMKKKGKK"
     gene            complement(4500..>4944)
                     /locus_tag="PK198_17990"
     CDS             complement(4500..>4944)
                     /locus_tag="PK198_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRF40690.1"
                     /translation="KTIAMASVATSAHEAYNYGYLLPGRDAVAPNKDRAIAEAKKKVL
                     ELSYNYVQPIQRNDVYVLGRQGLATLLLAANELRLGNYASDHDIKIAQKIAYVLCGGD
                     LTAPQQVSEQYLLDVEREAFLSLCGEQKTLERIQHMLQTNKPLRN"
     assembly_gap    4945..4993
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4994..>6388)
                     /locus_tag="PK198_17995"
     CDS             complement(<4994..>6388)
                     /locus_tag="PK198_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRF40691.1"
                     /translation="PDTDPAIVDFLMHYGDVQLGKQTVLCKDTPAFIANRVGVYAMAK
                     IYQLTTELGLSIDEVDKLTGPAIGRPKTGTFRLGDLVGHDTAAKVIMGLKQNCPNDEQ
                     AATFEIPKYLQFLLDNNFLGNKSGQGFYKKTREKDANGKDIILALNLDTLEYQPTQKS
                     SLASLGLSKQIDSLAKRLPALVDAPDKGGELLRRSLMGLFAYVSNRVPEISDNLYSID
                     DAMRAGYAWELGPFEYWDIIGVAKGMELAEAQGEIIAPWVKEMLAAGHTSFYQRQQGR
                     RLYYDAGSRSYLPIPGLDSFIILNDLRDAAPVHTTSETVLHDIGDGVLCLEFTSMHNT
                     IGEGVLRGINEAIQIAEDGDWRGLVIGNNATNFSVGANLMMIAMLAYQQEFDDLNMAV
                     NLFQQTTMRCRYSSIPVVVATQGYVFGGGCETLMHCDAAVCAAESYIGLVEVGVGLLP
                     GGGGTKEFSLRASDA"
     assembly_gap    6389..6582
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6583..>6791)
                     /locus_tag="PK198_18000"
     CDS             complement(<6583..>6791)
                     /locus_tag="PK198_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRF40692.1"
                     /translation="LPPNLSDAEKSNRAARNRMADTALANALKSKPAPLYDKKFASRI
                     QTGNFEDDMARIKDCDWVIEVVVER"
BASE COUNT         1357 a         1679 c         1955 g         1530 t
ORIGIN      
        1 gttcccatca acacaccagt tattgccacg cctgccaaca tcaccaatgt gaactgtttc
       61 ggtgcctcta ccggcgccat tgacatcagc gtgtctggtg gtacgggcaa ttatacttac
      121 atgtggagtt accaaaacag aactacgcaa gatttgacca acatcccggc gggcacttat
      181 tctgtgaccg ttaccgatac gggtaccaac cagactacga gcggttcgtt caccgtcaca
      241 cagcctgttt cagggatttc cattattgcc attcctacca atgtagcttg taccggcgct
      301 tcaaccgggg taattacgtt gaacgtaagc ggtggtacag gttcttataa ctacaactgg
      361 agcggcacct tgcccgatgg cgtaccgagc caaagtaatc tcgccgctgg tacatattcg
      421 gtgaccgtta ccgacggcaa caactgtacg gcttctcaaa gcgtcacgat taccgagcct
      481 gccggcacgc ctctgaccat cagcagtatg gtgcaaccgg tggtttgcgg cggcgcttct
      541 acgggtagaa tcacattgac catttccggg ggcacgccca attattccat caactggtgc
      601 tgtggccttc cggccaatgc cacgacggtt tccaacctga ctgccggcac atacaacgtc
      661 acggtaacgg acaacaacgg ctgtacggcc actcgcagca tcaacgtggg cgagaaccct
      721 gtgttgcaaa tcagcaccat cacacctacg cacatcaata acggcaatga cggcgccatc
      781 aacatcacgg tgacgggcgg aacgggcggt tatagctttg tgtggaccgg ccccgcaggt
      841 ttcaacggct ctaaccagcc caacctgagc gggcttaatt tccccggcca atactgcgta
      901 accgtgaccg acaacagcgg ttgtaccgca agtcgttgcg tacagttgga tgagcgcttg
      961 cgttttggca acgtggtcat cgggcgttct tgcgcgggcg cttcctccgg tagtattcag
     1021 gtgagcgtgc tgggaggcgt ggggccttac aattataact ggaatcctgg aggccaggct
     1081 ggctccaacc ggaccaacct cgctgcaggc acctacaatg ttacggttac cgatcaggta
     1141 ggaggagtgc tggccggcag ctttgtagta gaaaacttcc ccacggccgt agtatcgggc
     1201 acggtagtga atgcaagcgg caatattacg gatgccaatg gttctgtaac gctgaatatc
     1261 tcaggaggca ctccaggtta taccatcaac tgggcgccgg gcggcactac cggattggtg
     1321 ttgagcaatg tagtaacagg gcagtactgt gctaccgtga ccgatatggc cgggtgtagc
     1381 gtggtgcaat gttttacggt tggcttcaat ccggggccat tggccattgt caatacccaa
     1441 acaactcccg taacctgccc cggtgatcag aatggcgccc tgaacatcac gctcaatgga
     1501 ggtttgcctc cgtttacttt tgccttcagc gacgggttta accaaacgat aaatggcgta
     1561 accctgaacc gcagcaacct gccggccggt gcgctcacct atacagtaac ggatgctcag
     1621 ggcacggtta tcaatggctc tgccaccatc acagcttcgt cggtagtgac taccacaaac
     1681 attgccgtgg ttcacgattc ggaagagcct ggctgtaccg gttctgtaac gctgaccctg
     1741 acaggcggaa cgctgcctta ccagttgcag tggaacacgc ccaataccgg cggcccggcc
     1801 atcaataacc tatgcgcagg tactttcatt gccacggtga cggacggcaa cggttgtatt
     1861 ttcgtactgc ccggcatcga agtgaataca tttggacttt cgtcaacttc tacggctacc
     1921 aactgtccgg gagacgctac cggcgccatt aacctggttg tgaccggtgg ccgagcgccc
     1981 tacacttacg agtggcgcaa tgccgccgga gccattgtct ccacagatga agaccttgcc
     2041 aatgcagcgc ccggcactta cacggtgcgc gttaccgagg ggtcgggcaa tgtgctgacg
     2101 cgtacttaca ccattgcttc gcaatcagcg ctggctttgg atgtggacgt gacgagcaat
     2161 tacaacggtt ttgacgtgcg ttgccacaac agcacagatg gctcaatggc ggctactgcc
     2221 ttcaacggtc agggtaatta tacctttgaa tggcgccgca acggcacagt agtgggcggt
     2281 tcgggggcaa cgctgaccaa tgcagctccc ggcgaatatc tcgtgcgcgt gaccgacggt
     2341 ttaggctgta ccgtgacgga aacccttact ttacaggcgc cacagcctgt gacggtgacg
     2401 gccaataccc tgccgacgag ttgtacgggc gtcaacgatg gccagatttt tgcggctgca
     2461 tccggagggg tatttgccct gccgtacaac tacgaatgga gcaatggcgc ttttggccct
     2521 cgtcttacgt tcctgcgcgt cggttcttat acggtcactg taaacgacgt gaacaactgt
     2581 accgccacgg ctactttttc agtaagcaat cccgccccga tcgtagtaac tgtgaacagc
     2641 acgccggcta ccgatggttg caacggctcg gtgctggcag tagtgacagg aggcggagag
     2701 gggccgttta cgttcacttg gtcaaccggg caagtgacgg cagaaccctt cctcacaggt
     2761 ctgtgtcccg gcgagtactt tgtaatggta accgacagcc gcggctgccg tcctgatcct
     2821 aaccttgctt ccggcactgt aagagaccgc aggacgccct gcctcgacat tcgcaccgtt
     2881 attacgcccg acggcgacgg cttgaatgag gagttcatca tcagttgtgt ggatgaattt
     2941 gccaataacc atctggaagt ttacaaccgt tggggtcagt tggtatatga aacggacaac
     3001 tacgacaaca cctggagagg cactacgcgc aacggcagcg aactgcccga tgggccgtat
     3061 tattttgtgt ttgaatacac cgacatagac ggcatcaggc agcaattgag aggttccatc
     3121 acgattttgc gcagataaac cgaaacaatg cggtgcgatg cagtacaatg aagactccgt
     3181 cgcaccgcac atctcaaaaa ccatttttca ccatcaaccg attccaaaaa catgaaaaag
     3241 attcttttac tctccataat cgccgttgca gccttgatgc aggttcaggc gcaggatcag
     3301 gcaatttttt cgcattacaa catcaaccct attttgattc atccggcagc ggccggcttt
     3361 agcgggcaac accagttgtt gttcaatgcg cggatgcaat ggacgggctt cccggatgcg
     3421 cccaaaacct ttatggcgca gtacaatggc ccgttgagca agagtttcgg cattggtttc
     3481 ggcgtgttgt cggaatcggc ggcgcaattg caaaggacgc gggcgcaact caattatgcc
     3541 attcgtttcc ctttgggtaa ggactgggaa atggcggcag gtttttctac cgaattgcag
     3601 cagatcagat tgcatggcga tgtggccggc ggcagtttcg ttgatttann nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnttgat gccaatatga gcggcgttcg ttttttggat gcttcggtag
     3721 gtatttacgg cacttacaaa gaaaacacat tcggtggcct gtcttttacc aatttggtgc
     3781 gctcccgatt ggacggcttg gtagtatctg atgagaagga gtccctgttt aactactatg
     3841 tgttttatct ggggcacaag tttgaggtag aagacctgaa gtttacactc gaaccctctg
     3901 tgatggctcg ccagatcaga gatgtgccgt ttcagttgga catcaacctg aaggccggct
     3961 ttttggatga tcagttgctg gccggcgttt cgtatcgttc tttgggtgtg atgggggtga
     4021 tgctgggcgt gcaggtgtcc ccgttcaact tatactacac ctacgatgtt tcgtttcaac
     4081 gtttccagca gtttaattcc ggtgcacatg aagtcaccat tgcgctgaac atgaagaaaa
     4141 agggtaaaaa gtaacccgcc ccggtaaaga ttatatcggt ttttgggata gcctctctct
     4201 gcttcggcgg ggaggggcct tttttttgta gcggccatag acggtcggct gcccatgcgc
     4261 tcggtgggca gcgagttatt gctggccgat gtgtatgaac acattgagtt tccggtgcgg
     4321 acagcaggcc ggcagttgcg gaatccccaa aagtaagccg gtattgtgac tgccccgcgc
     4381 gccaggggca gaaatgaaaa tggccgctgc gcagagccgt tagggttgca cagcggccat
     4441 tgtaatggat gacccgcaat aagaacttgc ccgtggcggg gtggcgccct attccatcat
     4501 cagtttctca acggtttatt ggtttggagc atgtgttgga tgcgctccaa tgttttctgt
     4561 tcgccgcaga ggctgaggaa ggcttcgcgt tcgacgtcca agaggtattg ttcggagacc
     4621 tgttgcggag cggtgagatc gccgccgcag aggacgtagg cgattttctg agctattttg
     4681 atgtcgtgat cgctggcgta gttgccgagg cgcagttcgt tggcggccag gaggagggtg
     4741 gccaagccct gtctgccgag gacatacacg tcgttgcgct ggatgggctg cacatagttg
     4801 taagacagtt cgagcacttt tttcttggct tcggcgatgg cgcggtcttt gttgggcgct
     4861 acggcatcgc ggccgggcag gaggtagccg tagttgtagg cttcgtgggc cgaggtagct
     4921 acggaggcca tggcgatggt tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnagcgtcg gaggcgcgca gggagaactc tttggtgccg ccgccgccgg
     5041 ggagcaggcc tacgcccacc tcgacgaggc cgatgtaaga ttcggcagcg caaacggctg
     5101 catcgcagtg catgagggtt tcgcagccgc cgccgaagac gtagccctgc gtggccacga
     5161 ccaccggaat ggaggagtag cggcagcgca tggtggtttg ctggaagagg ttgacggcca
     5221 tgttgaggtc gtcaaattct tgctgatagg ccagcatggc aatcatcatg aggttggcgc
     5281 ctaccgagaa attggtggca ttgttgccga tgacgaggcc cctccaatcg ccgtcttcgg
     5341 caatttggat ggcttcgttg atgccgcgca gcacgccttc gccgatggtg ttgtgcatgc
     5401 tggtgaattc gaggcagagc acgccgtcgc cgatgtcgtg gaggacggtt tcggaggtgg
     5461 tgtgtacggg cgccgcgtcg cggaggtcgt tgaggatgat gaaagaatcg aggccgggaa
     5521 tgggcaggta gctgcggctg ccggcatcat aatagaggcg gcggccctgt tggcgttggt
     5581 agaatgaggt gtggccggcg gcgagcattt ccttgaccca gggggcgatg atttcgcctt
     5641 gtgcttcggc cagttccatg cctttggcca cgccgatgat gtcccaatat tcaaaagggc
     5701 cgagttccca ggcatagccg gcgcgcatgg cgtcgtcaat gctgtagagg ttgtcggaga
     5761 tttcgggcac gcggttggag acgtatgcga agaggcccat gagcgagcgg cgcagcaatt
     5821 cgccgccttt gtcgggcgcg tccacgaggg cgggcaggcg cttggccaat gaatcaattt
     5881 gcttgctgag gccgaggctg gcgaggctcg acttttgggt gggctggtat tcgagcgtat
     5941 ctaagttgag cgccaggatg atgtctttgc cgttggcgtc tttttcgcgc gtttttttgt
     6001 aaaaaccctg gccggattta ttgccgagga agttgttgtc gaggaggaat tggaggtact
     6061 tcggaatttc aaaagtagcg gcctgctcgt cgttggggca attttgcttc aggcccatga
     6121 tgactttggc tgcggtgtcg tgacctacca gatcaccgag gcggaaggtg ccggttttgg
     6181 ggcgaccgat ggccgggccg gtgagtttgt ccacttcatc aatgctgagg cccaattcgg
     6241 tggtgagttg gtaaattttg gccatggcat atacgcccac acggttggcg atgaacgccg
     6301 gcgtgtcttt acacaacaca gtttgtttgc ccaattgaac gtcgccgtag tgcatcagaa
     6361 aatcaacgat ggcggggtcg gtgtcgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacgctct acgacgacct
     6601 cgatgaccca gtcgcagtct ttgatgcggg ccatatcatc ttcgaagttg ccggtttgga
     6661 tgcgggaggc gaatttttta tcgtagagcg gcgcgggctt ggatttgagg gcgttggcca
     6721 gcgcggtgtc ggccatgcga ttgcgggcgg cgcggttgga tttttcggcg tcggagaggt
     6781 tgggcgggag g
//
LOCUS       DAORLF010000262         6786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6057_length_6786_cov_1.790670, whole genome shotgun sequence.
ACCESSION   DAORLF010000262 DAORLF010000000
VERSION     DAORLF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_6786_cov_1.790670"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK198_18005"
     CDS             <1..278
                     /locus_tag="PK198_18005"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF40693.1"
                     /translation="RDGFFVQALYMTPWKVQPVVRYERFDPNMEMAADQLGFLPEDGM
                     QNVITYGLNYFFNEKVRFQINYLYKAEETGKLEVKNDILLTQFQIVF"
     gene            332..1189
                     /locus_tag="PK198_18010"
     CDS             332..1189
                     /locus_tag="PK198_18010"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRF40694.1"
                     /translation="MTILNKISTLIFLVLLSVLQLSAQQDCLSAEDFIALTKANPNLV
                     IVDANRAKTYQTNHIKGAIHINHMDLYQEGEIKGLLKSPEELAAFFGQKGISEKSSVV
                     LYDDASHKYTTRIYWTLKYLGATDVKILSKDMEAWKKVRLPLTTEVVALKPVKFTPAI
                     SAGMLATFEEVVKAEGQPGIVLVDSRNADEYAGKTEDSKGHIPGAVNLDQALMLTETG
                     YFKPAVELKAIVEAAGITPDKEVIVYCVTGVRASVLYYAFKNVLGYEKVKVYDGSYNE
                     WVAKKDVVN"
     assembly_gap    1411..1506
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1534..>1800
                     /locus_tag="PK198_18015"
     CDS             1534..>1800
                     /locus_tag="PK198_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40695.1"
                     /translation="MLIALFVGHQLLAQDAASFQNQCFRWSEKSGGRLSISLDDAGKF
                     EWKGSHLHSCTSKLNVYGRGCYLVVGDSVLLRFDSIPNRNSECKI"
     assembly_gap    1801..1812
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1813..2222
                     /locus_tag="PK198_18020"
     CDS             <1813..2222
                     /locus_tag="PK198_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40696.1"
                     /translation="ITLGADQDSSLDVDISVWDEESLPMNDLTFSWGISKKKGRRTIP
                     EFHMRKFDKSIYHSFGLLTRFNYLRIEKEGFYAADIDAKAINSDALIHVIMRHKPDVD
                     AFEYICNTELVLLILGNELVFNNEMIFVRQSCR"
     gene            complement(2230..4110)
                     /locus_tag="PK198_18025"
     CDS             complement(2230..4110)
                     /locus_tag="PK198_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40697.1"
                     /translation="MQRSIRRLLLWGIVALGIGVLIRSLQQDNTTPAHHPGTPGQARE
                     ILGSDESCMGCHGDMTGFETSHQPRLIGCTPCHLGNARAFDKDKAHADMVLVPGNQSN
                     VYQTCGTANCHGDIALRVENSLMSSMSGVVSVNRFVFGEQDSPNGFAHIRDIGTASAA
                     DTHLRHLCASCHLGNEKTHPAPVGERSRGGGCIACHLQYNADAMASLQSYQTDKALLP
                     RVHPAINLQVGNEHCFGCHSRSGRISTNYEGWHETTLQPDEVAGQPGFRLLEDGRVFR
                     QMPPDVHHTAGLACIDCHSSAEVMGDGQRYAHEEDAVKMRCEHCHFSGRPPTLPYDSL
                     DLESKKIMALRGVAGQGKRFVHGIWNVQMSESGIPFLLSKNTGKRHPLPAPAEVCSRQ
                     GAHASLSCSACHTSWAPQCVGCHTTYRPDLQAFDLLDKKKTPGKWEEHLGEFFAEAPT
                     LGVVQHSSVSAEQVRHIQTFIPGMIMTLDKSAFTGRQQAADFHRLFAPSASHTTSAKG
                     RSCQSCHSQPLALGYGRGRLDFVQQGRQGRWFFYPEYATDPHDGLPQDAWIGFLQEPK
                     RRASTRSNVRPFSVAEQQRILAVGACLQCHKDQSKVMQEALRDFEGVKRGMSGRCAVP
                     VW"
     gene            complement(4114..4566)
                     /locus_tag="PK198_18030"
     CDS             complement(4114..4566)
                     /locus_tag="PK198_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF40698.1"
                     /translation="MKIQDEIRQKKFKSEYQKAHINILFTAAWLSQQTVQILKPLGIS
                     SQQFNILRILRGMHPEPSSVKLLTERMIDKMSNASRLVDKLEAKKLVQRIESGADRRR
                     VDVFITPAGLQLIETASIAVEDRLHKDMELLSEEEAFRLNELLDKMRG"
     assembly_gap    4727..4833
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4834..6429)
                     /locus_tag="PK198_18035"
     CDS             complement(<4834..6429)
                     /locus_tag="PK198_18035"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40699.1"
                     /translation="MRLYILPLLVCMMLAVPAVAQRQFILNGQARIINDSCYQLTPET
                     NFVAGSIWNSDKISLLQSFDVVARVYLGCKDGNGADGMVFGFQPVSTSIGTAGGGIGF
                     ANIVPSIGIEIDTHYNSDFGDPTYDHIAITRNGNVNHNSGNTLAGPVQARATSSDIED
                     CKYYDLRVSWNAPLKRLQVYFDCVLRLTYTGDIVNQVFGGDPWVFWGFTAATGGLNNV
                     HQVCFEYTSFLDQLEDVTICPGAHYPLRIRGGASYRWSPETGLSNPNIANPIASPEST
                     TTYTVEVLDACNRPFYDSITITVSGDSLLVNLQSDSILCEGTTQNLDAGASGDRYSWS
                     TGEQTRFLPVTQPGTYEVTVSWNNPFCISVDEVTLVSKPQPQASLGRDTTLCEGQSIR
                     YLASFPEATYLWSDGSAADTLWVRAPGVYTLAVSNDCGTAIDGVRVDYENCREVYFPN
                     AFSPNDDGINDTFGPLHGGDVSEVHLLRVFDRWGGLVFEWKGDSDRLAWDGRLKNKPL
                     PQGVYVFWADLTFRDGHRAAFSGE"
     gene            complement(6429..>6786)
                     /locus_tag="PK198_18040"
     CDS             complement(6429..>6786)
                     /locus_tag="PK198_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF40700.1"
                     /translation="RLHRRSQVIGRTLKREIASRVFNGLLKVYLGVGFSDGMCGFKFL
                     QRSVYQHLYENGARNNGWFFSTELLTISEWLGLKIYELPVKWTDDAASSKVRIIPLAR
                     RYWKDMQALKQLKLHR"
BASE COUNT         1507 a         1721 c         1832 g         1511 t
ORIGIN      
        1 aacgcgacgg cttcttcgtg caggctttgt acatgacgcc gtggaaagtg cagcccgttg
       61 ttcgctacga gcgcttcgac ccgaacatgg aaatggctgc cgaccaactc ggtttcctgc
      121 ctgaagacgg catgcaaaac gtcatcactt acggtctgaa ctacttcttc aacgaaaaag
      181 tgcgtttcca gatcaactac ctgtacaaag cagaagaaac cggcaaatta gaggtgaaaa
      241 acgacattct cctcacgcag ttccagattg tgttctgata ctcgccactc acccactgca
      301 ttcattcact caccactgaa aaatttcagc catgacaatc cttaataaaa tctccacgct
      361 catttttctt gtactactct ctgtcctgca attatcggca caacaagatt gcctttctgc
      421 cgaggatttc atcgcgctta ccaaagccaa tcccaatctc gtgattgtgg atgccaacag
      481 agccaaaaca tatcaaacca accacatcaa aggcgccatc cacatcaacc acatggattt
      541 atatcaggaa ggcgaaatca aaggcttgct gaaaagtccg gaagaactgg cggctttctt
      601 tggtcaaaaa ggcatcagcg aaaaaagctc cgtggtattg tacgacgatg cttctcacaa
      661 atatactacc cgcatttact ggacgctcaa gtaccttggc gctaccgatg tcaaaatttt
      721 gagcaaagac atggaggcct ggaaaaaagt gcgcctgccg ctgactaccg aagtggtggc
      781 gctcaagccg gtgaaattta caccagctat cagcgctggt atgctcgcta cttttgaaga
      841 agtggtgaaa gcggaaggtc agcccggtat cgtgctggtg gacagccgca atgccgacga
      901 atacgccggc aaaaccgaag acagcaaagg ccatataccc ggcgccgtca acctcgacca
      961 ggcactcatg ctcaccgaaa cgggctattt caaacccgcc gtcgaactga aagccatcgt
     1021 ggaagccgcc ggcattacgc ccgacaaaga ggtgattgtt tactgtgtga ccggcgtgcg
     1081 cgcatcggtg ctgtactacg cgttcaaaaa cgtgctgggc tacgaaaaag tgaaggtata
     1141 tgacggcagc tataatgaat gggttgctaa gaaagatgtg gtgaattgat cggcagcaat
     1201 tagccgcgag cttcgagttg ctaagcgaaa gttcggcagc ctgaaaatca cctgaaaaaa
     1261 gaatcaaaat gctgtcgccc ggcccttcaa cgggcggcag cccccaagaa taaggtgaaa
     1321 gaagattagt ttttttatgg caaggtagcc ctgtcatcgt ttcggcgaag gcagggcttc
     1381 tgcttgtgtg gggagggaat gtgatatcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnatga aatgtacttt tcaaatgtta ctcatgctca ttgcattgtt cgtcggccat
     1561 caactgcttg cccaagatgc ggcttcattt caaaaccaat gctttcgatg gtcggagaaa
     1621 agtggagggc gcttgagcat ttcattggat gacgccggta agtttgaatg gaaaggaagt
     1681 cacctgcata gttgcacctc aaaactgaat gtttacggca gagggtgtta tcttgtcgtt
     1741 ggcgactctg ttttgttgcg atttgattcc attcccaaca gaaattcgga atgtaaaatc
     1801 nnnnnnnnnn nnaaatcacc ttaggtgctg atcaggattc gtcgttggat gtggacatca
     1861 gcgtttggga cgaagagagt ttgccaatga atgatttgac tttttcctgg ggtatttcta
     1921 aaaagaaggg caggagaacc attcctgaat ttcacatgcg taaatttgat aaatctatct
     1981 atcattcatt cggccttctc acccgattca attatttgag aattgaaaaa gaaggattct
     2041 acgcggcaga catagatgcc aaggcgatca acagtgacgc gctcatccat gtaatcatgc
     2101 ggcacaagcc cgacgtggat gcttttgaat acatctgcaa tacagaactg gtgttgttga
     2161 ttttgggcaa cgaattagtg ttcaataacg agatgatatt tgtgaggcag agttgcaggt
     2221 aagcgccccc taccacaccg gcacagcgca cctcccactc atccccctct tcaccccctc
     2281 aaaatcgcgc agagcctcct gcatcacctt cgattggtct ttgtggcatt gcaggcaggc
     2341 gcctaccgcg aggatgcgct gttgttccgc tacactgaaa ggacggacat tgctgcgcgt
     2401 agaagccctg cgttttggct cctgcaaaaa accgatccag gcatcctgcg gcaagccgtc
     2461 gtgcgggtcg gtggcgtatt cgggataaaa aaaccagcgc ccctgccgcc cctgctgcac
     2521 aaaatcgagc ctgccccgac cgtagcccaa ggcgagcggt tgactgtggc aggactggca
     2581 actccgccct tttgccgagg tggtatgcga tgccgagggc gcaaaaagcc ggtgaaaatc
     2641 agccgcctgc tgtcgccccg tgaacgcgct tttgtcgagt gtcataatca taccgggtat
     2701 gaaggtctga atatggcgca cctgttctgc gcttacgctg ctgtgctgca ccacgcccag
     2761 cgtcggcgct tcggcaaaaa actcgccgag atgctcctcc cacttgccgg gcgttttttt
     2821 cttgtccaac aaatcaaaag cctgcaaatc aggccggtaa gtggtgtggc aacccacgca
     2881 ttgcggcgcc caggaagtat ggcaggcgct gcaactgagc gaggcatgag cgccctgccg
     2941 gctgcacact tccgccggag cgggcagtgg atggcgcttg ccggtgtttt tagacaataa
     3001 aaacggaatg cccgattcgc tcatttgcac gttccagatg ccgtgtacga agcgcttgcc
     3061 ctgtccggct acgccccgca gcgccatgat tttttttgat tccaggtcta agctgtcata
     3121 gggcagggta ggggggcgac cgctgaaatg gcagtgctcg cagcgcattt ttacggcgtc
     3181 ttcttcgtgg gcgtagcgtt ggccatcgcc cataacttcg gcggaactgt ggcagtcaat
     3241 gcaggccaaa ccggccgtgt ggtgtacgtc gggcggcatt tgtcggaaaa cgcgcccgtc
     3301 ctccagcagg cggaagcccg gttgcccggc gacctcgtcg ggttgcagag tggtttcgtg
     3361 ccagccctcg tagttggtag agattctgcc ggagcgactg tggcagccga agcagtgctc
     3421 gttgcctacc tgaaggttga tggccggatg cacgcggggc agtagggctt tatcggtttg
     3481 gtaagattgc agcgaggcca tcgcgtcggc gttgtattgc aggtggcagg cgatgcagcc
     3541 cccgccgcgc gagcgttccc cgacaggggc cggatgtgtt ttttcgttgc cgagatggca
     3601 ggacgcgcag aggtggcgca ggtgggtgtc ggcagccgag gccgtgccga tgtcgcggat
     3661 gtgcgcaaag ccgttggggc tgtcctgttc gccgaagaca aaacgattga cggaaaccac
     3721 gcccgacatg ctgctcatca gcgagttttc gacgcgcagg gcgatgtcgc cgtggcaatt
     3781 ggccgtgccg caggtttgat acacattgct ttgattgccg ggcaccaaca ccatatcggc
     3841 atgtgctttg tctttgtcga aagcgcgggc attgccgagg tggcagggcg tgcagccgat
     3901 gagtcggggt tggtgcgagg tctcaaaacc ggtcatatcg ccgtggcaac ccatgcaact
     3961 ctcatcgctg ccgaggattt cgcgggcttg gccgggtgtt ccggggtggt gagcaggagt
     4021 cgtgttgtct tgttggagag atcgaatcag cactccgatg ccaagtgcaa caatgcccca
     4081 caggagcagc cgacggatgc tgcgctgcat gttttaccct ctcattttgt ccaacaattc
     4141 attgagccgg aatgcttctt cctccgacag caactccata tccttatgca ggcggtcttc
     4201 gacggcaata gaagcagttt cgatcaattg aaggcccgca ggcgtgatga atacgtccac
     4261 gcggcggcgg tctgcgcccg attcgatgcg ttgtaccaat ttttttgcct ctaatttatc
     4321 caccaatcgg gaggcgttcg acattttgtc aatcatgcgc tcggtgagga gcttgacgga
     4381 ggaaggttcc gggtgcatac cgcgaaggat gcggagaata ttgaattgtt gagatgatat
     4441 tcctaaaggt ttgagtatct gtacggtttg ctggctgagc caggccgccg tgaacagaat
     4501 gttgatgtgg gctttctgat actcgctctt gaacttcttt tgccggattt cgtcctgtat
     4561 tttcatgctg tttcgagttt attatgcgta gcgaaggggt gactactgaa ttgaataccc
     4621 ggcagagtca ccccttcgct acactgccca aagccaattt gatccaaact aatacattac
     4681 tcatgcgctg cgcatgagcg ttttgaaatg ctcactataa tcatcannnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctctccg ctaaaggccg cccgatgccc
     4861 gtcgcgaaac gtcagatcgg cccaaaatac atacacgccc tgaggcaggg gcttgttttt
     4921 gagccgtccg tcccatgcaa gccggtcgga atcgcctttc cattcaaaca ccaaaccgcc
     4981 ccagcggtcg aacacgcgca acaggtgtac ctcgctcaca tcgcctccgt gcagcggccc
     5041 aaaagtatcg ttgatgccgt cgtcattggg actgaaagca ttggggaaat acacctcgcg
     5101 gcaattttca tagtccacgc gcacgccgtc tatggctgtg ccgcaatcat tgctcacggc
     5161 aagggtgtaa accccaggcg cgcgaaccca cagcgtatcg gcagccgagc cgtccgacca
     5221 aaggtaggtc gcctccggga agctcgccag atagcggata ctctgccctt cgcagagggt
     5281 ggtgtcgcgg cccagcgagg cttgtggctg cggtttgctc acgagggtga cctcgtccac
     5341 agagatgcaa aacgggttgt tccaggaaac ggtgacctcg taagtgccgg gctgcgtgac
     5401 gggcagaaaa cgcgtttgtt cgccggtgct ccaactgtat cggtcgccgc ttgcgcccgc
     5461 atcgaggttc tgagtcgtgc cttcgcacaa aatgctgtcg gactgcaagt tgaccaacaa
     5521 actgtcgccg ctcacggtga tggtgatgct gtcgtaaaac gggcgattac aggcgtccaa
     5581 cacctcaacg gtgtaggtgg tggtgctttc cggcgaagcg atgggattgg cgatgttggg
     5641 attgctcaag cccgtttccg gcgaccagcg atacgaggcc ccgccccgga tgcgaagcgg
     5701 ataatgcgct cccggacaaa tggttacgtc ttctaactgg tctaaaaaac tggtgtattc
     5761 aaaacaaacc tgatgcacgt tattgagtcc gcctgtagct gccgtaaagc cccaaaatac
     5821 ccagggatcg ccgccgaaaa cttggttgac aatgtcgccg gtgtaggtga gccgcagtac
     5881 gcagtcgaaa tacacctgaa gccttttcaa cggtgcgttc caactgaccc ggagatcgta
     5941 atatttgcag tcttcgatgt cggaactggt tgctctggcc tgcacaggtc cggcgagggt
     6001 gttgccggaa ttgtggttca cattgccgtt gcgggtgatg gcgatgtgat cgtaggtggg
     6061 gtctccgaaa tctgaattat agtgggtgtc tatttcgatg ccgatggagg gcacgatgtt
     6121 ggcaaaacct atgccgcctc ctgccgtgcc gatggacgtg ctgacgggct gaaacccgaa
     6181 caccatgccg tcggcgccgt tgccgtcttt gcagccgaga tagacgcggg ctacaacgtc
     6241 gaacgattgc aacaggctga ttttgtcgct gttccagata gagccggcca caaaattggt
     6301 ttcaggagtc aattgataac acgaatcgtt gatgatgcgc gcctgcccgt tgaggataaa
     6361 ctgccgttgc gcaacagcgg gaaccgccag catcatacag acgagcaatg gtaaaatgta
     6421 tagtctcatt accggtggag tttaagttgt ttcagtgcct gcatgtcctt ccagtagcgg
     6481 cgggccagcg gaatgatgcg caccttgctg gaggcggcat catcggtcca tttcacgggc
     6541 agttcataga ttttcagtcc cagccattcc gagatggtga gcagttcggt agaaaaaaac
     6601 cagccgttgt ttcgggcgcc gttttcgtaa aggtgttgat agacagatcg ttgtaggaat
     6661 ttaaacccgc acatgccgtc ggagaaaccc acgccgagat agactttgag caagccgttg
     6721 aacacgcggg aagcgatttc ccgtttcagc gtgcgcccga tgacctgcga gcggcggtgc
     6781 aggcgg
//
LOCUS       DAORLF010000263         6782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6065_length_6782_cov_2.022298, whole genome shotgun sequence.
ACCESSION   DAORLF010000263 DAORLF010000000
VERSION     DAORLF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_6782_cov_2.022298"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1571
                     /locus_tag="PK198_18045"
     CDS             69..1571
                     /locus_tag="PK198_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40701.1"
                     /translation="MKTPASDLHNLIQSLNAAEKRYFRLFAARHPADGQSNYLRLFDL
                     IAVQEEYDEAALKSSLEADPLLRHFAVTKRYLYDQILECLHQFHAVQSVEEAIKKQVH
                     LASVLLQKDLPKQSEKLLQQARRQIDKYELLSLMPEWLTAQRAVQDRLRAADAQAVMA
                     WKNDADAALKKLSVETDYAALSAIVGLWHVRKVSAQDNSLQEYLHQNELWPLLSEEAH
                     PPTLRAQLDGFKARATWHFMRSEPQEAYRLNLAHLALLDEHPELLQLYPRRYLAALNN
                     LLIDQFQLRLYEELEGGIQKLKSLLERPEFRRVPKLEEKIFEQSALLRLNSLLARRQY
                     AEALEALPAIEADMQRLSAGISPHLLLSIQFLIARLYFENERFGSALHWLNALLQHPG
                     RQLMEELFRFAGILKLLVHFELEHDELLPYLIESARRQGVLSGRRYQAENLCFKYLKK
                     LLQATGSKGRQRIFTLWKNDLLPLAAITAEQRIFNYIDFNQWLEKKHPTT"
     gene            1568..2518
                     /locus_tag="PK198_18050"
     CDS             1568..2518
                     /locus_tag="PK198_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF40702.1"
                     /translation="MKPAALPERPPSPLQEIRHTELDAKGLRLLVKRDDLLAWSKNSA
                     LCGNKWRKLKYNLLEAKTTHQTHLLTFGGAYSNHLAAAAEAAKVLGFEATGIVRGEET
                     LPFNPTLAFVKQCGMRLVYVNREAYRYKSEPDFLPSIGIDATGVFVLPEGGTNMAALR
                     GCRELAEEIQAQAEADFIAVACGTGGTLAGIVSGLKESCKALGFSVLKGDFHRKEVAA
                     LLLEAGAMPADNWQVETAYHFGGYAKWKPELLDFMQEFRDQYGITLDPIYTGKMMYGV
                     FDLIRNGAFPRGSTIVAVHTGGLQGVAGFEQRFSASGVSV"
     assembly_gap    2592..2664
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2665..4256)
                     /locus_tag="PK198_18055"
     CDS             complement(<2665..4256)
                     /locus_tag="PK198_18055"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF40703.1"
                     /translation="MSDQHTHPLPLGSSPDHGAAHENDHRQWSRRSFLRSIGITGGVS
                     LMAGSMPLVALKGTFLEMALANSNTDRILVLIRLKGGNDGLNTIIPVYDYGTYQALRP
                     QLRVPSNQLIALNASFGMPQQMADLQPLWQDGAMKVVHGVGYPDQNLSHFRSSDIWAS
                     ASPANVIDDSGWLGRLLVEQYPDYLTNPPLIPPAVQIGGVGNISFMDGENVNLSVSVT
                     NPNELAEIAQLGQLYDIQNLPDCFYGEQLGYMRTVANSTFIYAQRIKDAYDNSTTDAV
                     YQTGSLWQQLALVARLIKGGLGTKLYMVTLDGFDTHAAQNQAHPLLLAQLAGAVRAFY
                     QDLTAAGRAGDVLCMTQSEFGRRPEQNASQGTDHGAAAPLFLFGEGLNGNGFVGTAPS
                     LQNLDNDGNLVFSTDFRRIYATVLENWLCIEPTLVDGILGQHFARLPLGLNCAATGIA
                     GEAAPGIPHEVRYISGREVVISYQLHSAAMVRVEVFNILGQPVALLSNGYQMSGRHEV
                     TFSQAGRYLPAGQYVYRISAGE"
     gene            complement(4281..5720)
                     /locus_tag="PK198_18060"
     CDS             complement(4281..5720)
                     /locus_tag="PK198_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF40704.1"
                     /translation="MINCNTGSIAPYIPDAQRPWNKRRVQHLYRRMGFGADNATITAG
                     LAQSPGALVDSIINNALALPLAPQPVWAFWTVANYQNQEQAVAQIFDWRRLWVRDMLA
                     NGFRGKLTLFWHNHFVTRLESYGCPSYFYQYHKVLQQYALGDFREFVRAVGLTPAMLV
                     FLNGVQNTRFQPNENYARELYELFTLGADNGYTQQDIVQTARALTGYNGLGEYCGPVS
                     YVPAFFDPLPKTIFGQTGNWGYNDVVNNLFQQRASQIAHYICRKIYRYFVSPNADETI
                     VAQMASTFLANSFQLAPMLRQLFKSEHFFDENIMGAEVKGPLEMLLTFVKETSYPNPT
                     DSDIDGILYLAAELGQDLANPTDVAGWPGNRSWINSNTLTGRWQAARFVIYSLYVNYP
                     QVLVNFAKAVSNNSNNAALVSRLIVDHFVPKDLQFETDYERSLEVFKAEIPDNYFQDG
                     QWNLEWPTAAAQVGLLMDHIIRKPDFQLF"
     gene            5962..>6782
                     /locus_tag="PK198_18065"
     CDS             5962..>6782
                     /locus_tag="PK198_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40705.1"
                     /translation="MNRFLILCSFLSLLPVWSYGQSFPSFADRPEWTYAYFIFGQAQT
                     PSVITMSEETMICGQLWNKLVTNNPGIAQQWPDTTSVGYIRTVGEKTYFRRSANCSRP
                     EWLLYDFSLVQGDTFHYRWHYPFDTTDLYAIQVPLVVYNVGWLDLGGVVRKVIDLAGI
                     YNDGINNYLATTWIEGIGALQEPFVSLLDTDSYSSEISFELMCARYGETVKYAAPQGP
                     CAYNYTRLHVKHDVQGGRNDGSNWANAFQRLEDAIAVADPGDSIWVARGTYYPTTG"
BASE COUNT         1590 a         1712 c         1848 g         1559 t
ORIGIN      
        1 cccgaaacct tgaacccgaa actccaaaat tccccctatc tttgctgctt aaaataaaaa
       61 ctctgatttt gaaaacgcct gcctccgatt tgcataattt gattcaatct ttaaatgccg
      121 ccgagaaacg gtactttcgg ttgttcgcag cccggcaccc cgccgacgga cagagcaact
      181 acctgcgcct gtttgatctc atcgccgttc aggaagaata tgacgaggca gccctcaaat
      241 ccagcttgga ggccgaccct ttgctccgcc actttgccgt caccaagcgc tatctctacg
      301 accagattct ggagtgcctg catcagtttc atgccgtgca atcggtagag gaggccatca
      361 aaaagcaggt gcatctggcc agtgttttat tgcaaaaaga cctcccgaaa caaagcgaaa
      421 aactactgca acaagctcgt cgtcagatag ataaatacga attgctcagc ctgatgcccg
      481 aatggctgac agcgcagcgg gctgttcagg atcgcttgcg ggctgccgat gcacaggcag
      541 tgatggcctg gaaaaatgat gccgatgccg ccctgaaaaa actctcggtt gagacagatt
      601 acgcggcgct ctcagccatc gtggggctgt ggcatgtgcg caaagtatcg gcacaggaca
      661 attctttaca ggaatatttg caccaaaacg agctgtggcc gctgttgtcg gaagaggcgc
      721 atccgcccac tttgcgcgcc caattggatg ggttcaaagc gcgcgctacc tggcatttta
      781 tgcgcagcga acctcaagag gcgtaccggc tcaacctcgc gcacctcgcc ttgttagacg
      841 aacacccgga attgctacaa ctttatcccc ggcggtatct ggcagcgctg aacaacctcc
      901 tcattgatca atttcaactt cggttgtatg aagaattaga aggcggcatc caaaaattaa
      961 aatcactgct ggagcgcccc gaattcaggc gtgttcccaa attggaagaa aaaatatttg
     1021 aacaaagcgc gttgctccgc ctcaattccc tgctggcccg gcggcaatat gcagaagcat
     1081 tggaagcact gcccgccatt gaagcagata tgcaacgcct cagcgctggt atttcacctc
     1141 atttgttgct cagtattcag tttctcattg cccgcttata ctttgaaaat gagcggtttg
     1201 gctctgcttt acactggctc aatgctctgt tgcagcatcc cggtcgccaa ctgatggagg
     1261 agttgtttcg ctttgcgggc atcctcaaac ttttggtgca ttttgaattg gaacacgacg
     1321 aactcctgcc ttatctcatc gaatcggccc ggcggcaggg cgtcctcagc ggcaggcgtt
     1381 atcaggcgga aaatctgtgt ttcaaatacc tgaaaaaatt gcttcaggca accggaagca
     1441 agggccggca gcgtatcttc acactgtgga aaaacgatct gctgccctta gcggcaatta
     1501 ctgccgagca gcgcatcttc aattatattg attttaacca atggttagaa aaaaagcacc
     1561 cgacgacatg aagcctgccg cattgcccga acgcccgccg agtcctttac aggaaattcg
     1621 gcataccgaa ctggatgcca aaggattgcg cctgcttgtc aagcgggacg acctgctggc
     1681 ctggtcgaaa aacagcgcac tgtgcggcaa caaatggcgc aagctgaaat acaatctgct
     1741 ggaggcaaag acaacgcatc aaactcacct gctcactttt ggcggggcgt attccaacca
     1801 tttggcggca gcggcagagg ccgcaaaggt actgggtttt gaagctacgg gcatagttcg
     1861 tggagaggaa acgcttccgt tcaatcctac tttggcattt gtgaagcaat gcggcatgag
     1921 gctcgtttat gtcaataggg aggcttatcg gtacaaatcg gaaccggact ttttgccctc
     1981 catcggcatt gatgccactg gtgtttttgt attgccggaa ggcggcacca acatggcggc
     2041 cttgaggggg tgccgggaat tagccgaaga aattcaggcg caagccgaag ctgattttat
     2101 cgcagtggct tgtggtacgg gaggcacgct ggcaggcatt gtttcgggat tgaaagaatc
     2161 gtgtaaagca ttgggattca gtgtgctgaa gggcgatttt catcgaaagg aagtggctgc
     2221 gcttttgctc gaagcaggcg caatgcccgc cgacaactgg caggtggaaa ctgcttatca
     2281 ctttggaggc tatgccaaat ggaagccgga attattggat tttatgcaag aattccggga
     2341 tcaatatggc atcacattgg acccgattta taccggcaaa atgatgtacg gagtatttga
     2401 tctcataagg aacggcgcat ttccaagagg cagcaccatc gtggcagttc atacgggcgg
     2461 actgcagggc gtggccgggt ttgagcagag gttcagtgct tcaggggtga gtgtgtgagg
     2521 ggtgagtgtg tgagggggga gcaacccgga accttgaacc cggaacccgg tgggcagtgg
     2581 gcggtaggcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnctttcg cccgccgaaa tgcgatacac atattgcccc
     2701 gccggcaagt agcgccccgc ctgcgagaac gtcacctcat gcctgcccga catttgatag
     2761 ccattgctca gcaaggccac cggttgcccc agaatattga acacctccac ccgcaccatc
     2821 gcggcactgt gcagttggta agaaatcacc acctcgcggc ccgaaatata gcgcacttcg
     2881 tgaggaatgc caggcgccgc ttctcctgca atgcctgtgg cggcacaatt caggcccagc
     2941 ggcaggcgcg caaaatgctg ccccaagata ccgtccacca aagtaggttc gatgcagagc
     3001 caattttcga gtacggtggc gtaaatgcgc cggaaatctg tgctgaaaac gaggttgccg
     3061 tcgttgtcta aattttgcaa actgggcgcc gtacccacaa acccattacc gttcaaccct
     3121 tcgccaaaga ggaacagcgg cgcggctgcc ccgtgatcgg tgccctgaga ggcattttgt
     3181 tccgggcggc ggccaaactc cgattgcgtc atgcacagca catcgccggc gcgaccggct
     3241 gccgtcaagt cctgataaaa tgcccgcacc gcgccggcca attgcgccag cagcagcgga
     3301 tgcgcctgat tttgagcggc atgcgtgtca aacccatcca gcgttaccat gtacagcttg
     3361 gtacccaatc cgcccttgat gagccgggcc acaagcgcca attgttgcca caacgagccg
     3421 gtttgataaa cggcgtcggt ggtggagtta tcataagcgt ccttgatgcg ttgcgcgtag
     3481 atgaaggtgc tgttggcaac ggttctcata tagcccaatt gctcgccgta aaagcagtcg
     3541 ggcaggtttt ggatgtcgta caattggccc aattgtgcaa tttctgccag ctcattcgga
     3601 ttggtgacag acacagaaag gttgacgttt tcgccgtcca tgaaagaaat gttgcccacg
     3661 ccgcctattt gtacagccgg cggaatcaac ggcggattgg tcaggtaatc ggggtattgt
     3721 tctacgagca gtcgccccag ccagccggaa tcatctatca cattggccgg gctggcactg
     3781 gcccatatat cggaggaccg gaaatgcgac aggttttggt cgggatagcc cacgccatgc
     3841 actactttca tggcgccatc ctgccaaagt ggttgcaaat cagccatttg ttgcggcatt
     3901 ccgaaggaag cattcagcgc tatcagttgg ttggacggca ctctcaactg cggcctcagc
     3961 gcctgataag tgccgtaatc atacacagga atgatggtgt tgaggccgtc gttgccgcct
     4021 ttgagccgta tgagtaccaa aatgcggtct gtgttggagt tggccaaagc catttccaaa
     4081 aaagtgcctt tgagcgctac aaggggcatg ctgccggcca tgagcgaaac gcctccggtg
     4141 ataccgatgc tgcgcaaaaa actgcggcgg ctccattggc ggtgatcgtt ttcgtgggct
     4201 gcgccgtgat cgggcgagct gccgagagga agtggatggg tatgctgatc ggacatgatg
     4261 ttttagattt gggtggtgaa tcagaacaac tgaaaatcgg gcttccggat gatgtggtcc
     4321 atgagcaggc ccacttgcgc ggcggcagta ggccactcca gattccattg gccgtcctga
     4381 aaataattgt ccggaatttc ggccttaaaa acctccagag atcgttcgta gtcggtttca
     4441 aactgaaggt ctttgggtac aaaatgatcc acaatcaggc ggctgaccaa tgcggcattg
     4501 ttggagttgt tgctgactgc tttggcaaaa ttgactaata cctgcgggta gttgacgtac
     4561 agcgaataaa tgacaaaacg cgcggcctgc cagcgcccgg taagcgtatt gctgttgatc
     4621 caggagcgat tgccgggcca gccggccacg tcggtggggt tggcgaggtc ttggcccaac
     4681 tcggcggcta gataaagaat gccgtcaatg tcgctgtcgg taggattggg gtaggaagtt
     4741 tcttttacaa aggtgaggag catttccagc gggcctttca cctcggcgcc catgatgttt
     4801 tcatcaaaaa agtgctcgct tttgaacaac tgcctcaaca tcggagccaa ctggaagcta
     4861 ttggccaaaa aggtggaagc catttgcgct acaatggttt cgtctgcatt gggactgacg
     4921 aaatagcggt aaatcttgcg gcaaatgtag tgcgcaattt gcgaggcgcg ctgctggaag
     4981 aggttgttca ccacatcgtt gtagccccag ttgccggttt gcccgaaaat ggttttgggc
     5041 agcgggtcaa aaaaggcagg cacatagctg acaggcccgc aatactcgcc caagccgttg
     5101 tagccggtga gggcgcgcgc cgtctgcacg atgtcctgtt gggtatagcc gttatcggcg
     5161 ccgagggtga agagttcgta cagttcgcgg gcgtagtttt cgttgggttg gaagcgggta
     5221 ttttgcaccc cgttgaggaa taccagcatg gccggcgtca gtccgacagc gcgcacaaat
     5281 tcccggaaat cgcccagcgc gtactgctgc aacaccttgt gatactgata aaaataggaa
     5341 gggcagccat aagattcgag gcgcgtgacg aagtgattgt gccaaaacag cgtgagcttg
     5401 ccccgaaacc cattggccag catatcgcgc acccagaggc ggcgccagtc gaagatttgg
     5461 gcgacagcct gctcctgatt ttggtaattg gccaccgtcc agaaggccca gacaggctgc
     5521 ggggcaagcg gcagggccag cgcgttgttg atgatggagt ccaccaatgc accgggcgac
     5581 tgtgccagtc cggcagtaat agtggcattg tcggcgccga aacccatgcg gcggtataag
     5641 tgctgtacgc ggcgtttgtt ccaggggcgc tgcgcatcgg gtatgtacgg cgcaatagaa
     5701 ccggtgttgc aattgatcat aggtgcaaag tgaaaatggt aagtaaatag tcagactgtc
     5761 gttgtggctg aaagttaaac gggcagtgga gagaattatt tttaatggca aaaaatgaaa
     5821 aatttaatac accgtctttt gctcgtgcga ctatttaaaa ctatttttgc cctcaccctt
     5881 gaagctcctt cccttgtgct gttccatcct gtatctgtat gacattggct gtattatttc
     5941 atcatcataa tctctcaact catgaacaga tttctaatcc tttgctcctt tttgagtttg
     6001 ctccctgtat ggtcttacgg ccagtcattc ccctcttttg ccgacaggcc ggagtggacg
     6061 tatgcttatt tcatctttgg acaagctcag acgccttccg ttatcaccat gtcggaagaa
     6121 acgatgattt gcggtcagct atggaacaaa cttgtaacca ataaccccgg catcgctcaa
     6181 caatggccgg atactacatc ggtgggatat atccgtacag taggagaaaa aacatatttt
     6241 cgaaggtcag caaattgttc ccgtccggaa tggcttctgt acgatttttc ccttgttcaa
     6301 ggcgatacgt ttcactaccg gtggcattac ccttttgata ccaccgattt atacgctata
     6361 caggttccgc tggtcgtgta taatgtagga tggttggact tgggcggcgt tgtgcgaaaa
     6421 gtcattgatt tagccggtat atacaacgat gggataaata attacctcgc caccacatgg
     6481 attgagggga taggagcttt acaggagcct tttgtgagcc ttttagatac ggattcatat
     6541 tcttcagaaa taagtttcga gcttatgtgt gccagatatg gcgagaccgt aaaatacgcc
     6601 gctccccaag gcccctgtgc ctacaactac acccgcctgc atgtgaagca cgacgtgcag
     6661 ggcgggcgca atgacggcag caactgggcc aatgctttcc agcgcttgga agacgccatc
     6721 gccgttgctg atcccggaga cagcatctgg gtggcgcggg gtacctatta ccctacaacg
     6781 gg
//
LOCUS       DAORLF010000264         6775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6073_length_6775_cov_1.852530, whole genome shotgun sequence.
ACCESSION   DAORLF010000264 DAORLF010000000
VERSION     DAORLF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_6775_cov_1.852530"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1597)
                     /locus_tag="PK198_18070"
     CDS             complement(41..>1597)
                     /locus_tag="PK198_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40706.1"
                     /translation="FKIKEEGRDFVPPDAARFLGRRTLRTPVETADIAGGGLLFYIFH
                     EQERSIPFSSLRFSPSRVMRMPGMPQPFAISPDENFDAFILTKTCGGYLKSSLDAGIE
                     PPYSSFKAALDTDSRRESSVMALSGSFLSPLRVALDGNDRRTAEAMMKLWQFYRDNPE
                     YVGRAYYLREFQGVMIKHISTAEENARIESEGAFNLSGPIPAKLKAAFSAGVSRQSSF
                     SGTDWQTILYADFDAAYRRESLFAPLPSPADIRRYFESIRPMAYLDGNSKALAEGVPF
                     LHVAELPGVPTEMTAQFWELESVAPGSFDNTPVLDAGQYTKGEQYGCRFSLSGRPARS
                     HFNPYDGQGSGIARLSYVIRSRQPVGGEYIRIQIDSETPVSIHPLAQVGRGEFDLGKK
                     ENRRFAVQWKMVIEVEDADNPVDFTVAPMLDKIELRNADGMLPIRVADVLPDASRRRF
                     HVVLESEETWPLERINDNDLQPYRLQMDIHLKSRRADTFAVRPVQGVVYLPAIRPEAP
                     ATMPTEGGGR"
     assembly_gap    1598..1642
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2042..>2855)
                     /locus_tag="PK198_18075"
     CDS             complement(2042..>2855)
                     /locus_tag="PK198_18075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40707.1"
                     /translation="YSTFFTINWPTYEGYIPNLGTGCTDIQLHLVAKLTSPVGKETAW
                     AARYRVNTAPAPYTYENTPPNPYLNVQETVEVPSIQDIINGNFQSWNPVVVTNDIVVT
                     GGAVENYLNSINAVRDHQDVLIQQFVRGQVIKAPVKFDLAPNCGSNPPVDADWLSSFC
                     ATPNRYNPILALRTPSEEEEPVVAHKLNLSVAPNPTGDATLLKYNLLEAGSVSIVLSD
                     YTGRRLAELHTASEQAAGEYQLLIPLDRYAPGIYVVTVARPSGKDSLRVVKH"
     assembly_gap    2856..2870
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2871..>3219)
                     /locus_tag="PK198_18080"
     CDS             complement(<2871..>3219)
                     /locus_tag="PK198_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40708.1"
                     /translation="GSNYTGQTSQARRPIYNRTLGILNVVEQPVMMLAVQPDVENPYP
                     DAPQIRFQSKSSLKLAAPLRYALNPASGLNLQDIRGALYFSCNTASNQRIHEGLIQEE
                     PGVWRTPYMPLSCL"
     assembly_gap    3220..3258
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3259..4194)
                     /locus_tag="PK198_18085"
     CDS             complement(<3259..4194)
                     /locus_tag="PK198_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40709.1"
                     /translation="MLLTQAMGLSQSDNITFPQFVLYNRFESKLRYFVYLAGVAQAND
                     VEVVVSFDRDGTETHISAALEHAFTPMDVVENYSDKRILITVPNSGFEIHGVWAMADI
                     PIAYDPCTCRFPSALRIGTKTNSYSNLNFTLDGGGDITQVISAPSGGGSGSSVLNAGR
                     RFANVTGSINTGISKGVSAYKTAGELAAVAEKLMLASANRKLNSNLLSTLNTISGISV
                     NSVTGISSAQANFLSQATNLLPGQADAIHQLFPKKVPTELVPDWLKTAIPFASSAFVL
                     LDFIIGGGKSTPPQPMHFNADFSFKGEGIISQSQNQ"
     gene            complement(4217..4468)
                     /locus_tag="PK198_18090"
     CDS             complement(4217..4468)
                     /locus_tag="PK198_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40710.1"
                     /translation="MFGKTTLIAAACLLLHLATASAQYCPGNPDGTSSFNWTAESWDV
                     WIRPNATLSPVLTTSLSLSSFYPSRSAQRSALRGICRYG"
     gene            complement(4616..>5145)
                     /locus_tag="PK198_18095"
     CDS             complement(4616..>5145)
                     /locus_tag="PK198_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40711.1"
                     /translation="VFTDTSFYLIFDNVSGWINFSLTTYNDKADATCHPGDSGKITVN
                     KAYYFKRYTYETDEDFVAEYPIFGTYLGADVESPQDTFQVTIDHWTQSLYRHTCIVNL
                     PRGCSRLCNAAYFNTHFFVAYALLPPHPDDLLCASPNRVFAELMPDNKTIEINYEFTS
                     GGQLVKRKWKGTKLY"
     assembly_gap    5146..5155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5156..5446)
                     /locus_tag="PK198_18100"
     CDS             complement(<5156..5446)
                     /locus_tag="PK198_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40712.1"
                     /translation="MKKSTLLPLLCLAAALPLLWTGCKKDPPCDDPLNPECPNYDRCL
                     TVPKTNAEFIVTDWTTPNEIPLPYRDTIPVASSVRVEFRAADRNMSRYEWRIG"
     gene            complement(5726..>5951)
                     /locus_tag="PK198_18105"
     CDS             complement(5726..>5951)
                     /locus_tag="PK198_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40713.1"
                     /translation="ARRRSRRYDTLMLHVLERGLHPGDELFRQLFERNPSPRLLAFLN
                     AETTLPQELALMSTTPIGKFLAALAGEMRR"
     assembly_gap    5952..6040
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6041..>6775)
                     /locus_tag="PK198_18110"
     CDS             complement(<6041..>6775)
                     /locus_tag="PK198_18110"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HRF40714.1"
                     /translation="RRVLILEPDTKDRNDRTWCFWEREPGPFEHIVHHQWRRLWFHQG
                     DFSRLLDINPYVYKVIRGIDFYKELKAVLAGFPNVEWRHELVEELRNTGAGVEVKAGG
                     WLYSADWCINSVYRGPAPDKQKLNYLDQHFRGWFVRAESDVFDPGVATMMDFRTPQEG
                     EFRFLYVLPFSTKEALVEVAIFSNQHLEAAGYDEILARYMREHLPQAGGYEVTETEQG
                     NIPMTDFDFPRQEGRIVNIGMAGGDTR"
BASE COUNT         1520 a         1688 c         1827 g         1542 t
ORIGIN      
        1 ttgaggcttg tggccagcag ctaacagcca gcagccgttc ctacctgccc ccaccctccg
       61 tcggcatcgt tgcgggcgct tcggggcgga tggccggcag ataaacgacg ccctgcaccg
      121 gcctgacggc gaaggtatcg gcgcggcggc ttttgaggtg tatgtccatt tgcaggcgat
      181 agggttgaag atcgttgtcg ttgatgcgtt ctaaaggcca ggtttcctca gattcgagga
      241 cgacgtgaaa cctgcggcgc gaggcatcgg gtagcacatc ggccacgcgg atgggcagca
      301 taccgtcggc attgcggagt tcaattttgt ccaacatcgg cgctaccgtg aagtccaccg
      361 gattgtcggc atcttccact tctatcacca ttttccattg tacggcaaag cggcggtttt
      421 cttttttgcc caaatcgaac tcgcccctgc ctacctgcgc cagcggatga atgctgacgg
      481 gcgtttcgga gtctatctga atgcggatgt actcgccgcc caccggctgc cggctcctga
      541 tgacgtaaga cagccgcgcg atgcccgaac cttgcccgtc gtaaggattg aaatggctgc
      601 gggcgggtct gccggagagc gaaaaacggc agccatactg ctcgcctttg gtgtattggc
      661 cggcatcgag caccggtgtg ttgtcgaaac tgcccggcgc cacgctttcc aactcccaaa
      721 actgagcggt catctcagtg ggtactccgg gcaattccgc cacatgcaga aaaggcacgc
      781 cctcggccag cgctttgctg ttgccgtcca aataggccat ggggcgaatg ctttcaaagt
      841 agcggcggat gtcggcagga gaaggcagag gcgcgaagag gctttcgcgg cggtaggcag
      901 cgtcgaaatc ggcataaagg atcgtttgcc aatcggtgcc cgaaaacgag ctttgccggg
      961 acacgcccgc gctgaacgcc gccttgagct tggccggaat ggggccgctg aggttgaaag
     1021 cgccctccga ttcgatgcgg gcattttcct cggcagtaga aatatgtttg atcatcacgc
     1081 cctgaaactc gcgcaggtag taagcccggc ctacatactc ggggttgtct ctgtaaaatt
     1141 gccacagctt catcatggcc tcggcggtgc ggcggtcgtt gccgtcgagg gctacgcgca
     1201 ggggcgagag aaaggagccg gaaagcgcca tcaccgacga ttcgcggcgg ctgtcggtat
     1261 ccaatgctgc tttgaaggaa gaataaggcg gttcaatgcc cgcatccaaa gaggatttga
     1321 ggtagcctcc gcaggttttg gtgaggataa atgcgtcgaa gttttcgtcc ggactgatgg
     1381 cgaagggctg tggcatcccc ggcatgcgca ttaccctgga gggtgaaaaa cggagactcg
     1441 aaaagggtat gctccgttcc tgttcgtgaa aaatgtagaa cagcagccca ccgccggcaa
     1501 tatcggctgt ttcaaccggc gtgcgcaggg tgcggcgacc caggaaacgg gcggcatcgg
     1561 gcggcacaaa atcccgtccc tcttctttga ttttgaannn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnagcgaact gcggctgtgt atgcgctgcg ggggccagtt
     1681 gtccatgcgc gatttttcca acttgtccaa tgctttttcg gcggtctggg cctgcaatgc
     1741 cggagtggct gccatgcaca aaaagagcgt gataccgatg ataaggctat gtattttcat
     1801 aaggcgtatg gttgcgtgtg atgcgccccg taaacgcctg agcaggggaa aagtattatt
     1861 gaaggctgtg atttttttat acccgataaa aaatggtttg cgaaaaaata taaaccattt
     1921 ttcatctgga taactgataa ccagtggttt gcaaaaacta aaattcgcaa accactggct
     1981 atcgagtata aatgagtagg cgaggctata actcgaagcc accgcctcac ctgcctgttt
     2041 atcaatgctt caccacccgg aggctgtctt tgccggaggg gcgagccacc gtcactacat
     2101 agatgcccgg cgcgtaacga tctaatggga tcaacaactg gtactcgccc gctgcctgct
     2161 ccgatgcagt atgcagttcg gccaacctcc gtccggtgta gtcgctcagt acgatgctca
     2221 ccgacccggc ttccaggaga ttgtatttca gcagcgtggc atcgccggtg ggattggggg
     2281 ctacggaaag gttcagttta tgcgctacca ccggctcttc ttcttcagac ggggttctga
     2341 gcgccagaat ggggttgtag cggtttgggg tggcgcagaa actactcagc caatcggcat
     2401 ctaccggcgg gttggagccg cagttgggag cgaggtcaaa cttgacgggc gctttgatga
     2461 cttggccccg gacaaactgc tgtatcaaaa catcttgatg atctctcacc gcgttgatgc
     2521 tgttgaggta gttttccact gcccctcccg tgaccacaat gtcgtttgtt accaccaccg
     2581 gattccatga ttgaaaattg ccgttgatga tgtcctgaat agagggtact tctacggttt
     2641 cctgcacatt gaggtaggga ttgggcggcg tgttttcata agtgtagggc gcgggtgcgg
     2701 tgttgacccg gtagcgggcg gcccaggcgg tttctttccc tacgggggaa gtgagcttgg
     2761 ctacgaggtg gagctggatg tcggtgcaac ctgtacccaa attaggtatg tatccctcat
     2821 aagttggcca attgatcgtg aaaaaggtgg aatagnnnnn nnnnnnnnnn caagcaagat
     2881 aaaggcatgt aaggtgttct ccatacccct ggctcttcct gaatgagacc ttcatgtatt
     2941 cgttgattgg atgcggtgtt acaggaaaaa taaagggcgc cccggatatc ttgaagattc
     3001 aaacctgatg caggattcag ggcatatcgc aatggcgctg ccaatttgag cgaacttttt
     3061 gactgaaaac gtatctgagg agcatcggga tatgggtttt cgacatcggg ctgcacggcc
     3121 agcatcatca ccggctgctc taccacgttg aggatgccca gtgtacggtt ataaatgggg
     3181 cgtcgggctt gtgaagtctg ccccgtgtaa ttgctgccgn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnntt gattttgaga ctggcttatt attccttctc ccttaaaact
     3301 aaaatccgca ttgaagtgca tgggctgggg tggcgtgctt ttgccgccgc cgatgataaa
     3361 atcgagcagc acgaaggccg agctggcaaa tggtatggct gttttcaacc agtcgggcac
     3421 caattcagtc ggcacttttt taggaaaaag ctgatggatg gcatccgcct gaccgggtag
     3481 caaattggtt gcctgactca aaaagttggc ctgtgcggag gagatgcccg tgacggaatt
     3541 gacgctgata ccggaaatgg tattgagcgt actgagtaaa tttgaattca gttttctatt
     3601 ggcagaggcc agcatgagct tttccgccac tgctgccaat tctccagcgg ttttgtaagc
     3661 cgaaacgcct ttggaaatgc ccgtattgat actccccgtc acattggcga aacgtctgcc
     3721 tgcattgagc acagagcttc ccgaacctcc accggaaggt gcgcttatga cctgggtgat
     3781 atcgccgccg ccgtcaagcg tgaaattcaa attggaataa gaattggtct ttgtacctat
     3841 tcgcaatgca gatggaaacc gacaggtgca agggtcataa gcgatcggga tgtcggccat
     3901 ggcccatact ccatgtatct cgaaaccgga attgggtacg gtgatgagaa ttcgcttgtc
     3961 gctataattc tccactacgt ccataggggt aaaagcgtgt tccaatgcgg cgcttatatg
     4021 cgtttcagta ccatctctgt cgaaggacac taccacttca acatcgtttg cctgcgccac
     4081 gcctgccaaa tagacaaaat aacgcagttt ggactcgaag cggttgtaaa gtacaaattg
     4141 cgggaaggtg atgttatcgc tttgagaaag ccccatcgcc tgtgttaata acacccatcc
     4201 gtccccgggt atgtaatcac ccataccggc agatgcctct aagtgccgaa cgttgggctg
     4261 accgagaggg gtagaaggat gaaagggaga gcgaagttgt aagcacaggc gagagggtgg
     4321 cattgggccg aatccataca tcccaacttt ccgcagtcca gttgaaagag gaagttccgt
     4381 cgggattgcc ggggcaatac tgcgcagatg cagtagccag atgcagcagc aggcaggctg
     4441 ccgcaatcag agtagttttt ccaaacatag taatgatggt ttatgaagtg aataaatagg
     4501 caataagagg gcgctttcgt cgcgagagca attgcgacga atgaagcgac acccgcaatt
     4561 agtgtggctg cggttcgaat gccgaatcag taatgcagac ttaaaatagc ggagattagt
     4621 acagcttcgt tcctttccat tttcgcttta ccaattgccc gccggacgta aactcgtagt
     4681 tgatttctat ggtcttattg tcgggcatta gttcggcgaa taccctgttg ggagaggcgc
     4741 acaacaaatc atccggatgc gggggcagca aggcataagc cacgaaaaag tgagtattga
     4801 aataagcagc gttgcaaaga cggctacatc ccctgggcaa attgacgata catgtgtgtc
     4861 tgtacaagga ttgggtccaa tgatcaatcg tcacctgaaa agtatcctgc ggactttcta
     4921 catcagcccc cagataggtg ccgaatatgg ggtactcggc cacgaaatct tcgtccgttt
     4981 cataggtgta tctcttgaaa taataggctt tgtttaccgt tatttttccg ctatcgccgg
     5041 ggtggcaggt agcatccgct ttgtcattgt aagtggttaa agaaaaattg atccagccgg
     5101 agacattatc gaatatcaga taaaatgaag tatctgtaaa cacccnnnnn nnnnngccga
     5161 ttctccattc ataccggctc atattgcggt cggcagcccg aaattctacc ctcacggagc
     5221 tggctaccgg aatggtatct ctatatggta ggggtatttc gttaggtgtg gtccagtctg
     5281 tcactataaa ctctgcattc gttttcggca cggtcaggca cctgtcataa ttcgggcatt
     5341 cgggattgag tgggtcgtcg cagggcgggt cttttttgca gccggtccac agcaggggca
     5401 gggcggcggc gaggcatagc agcggcaaga gtgtgctttt tttcatgtgt ttaactggtt
     5461 gttgcgggct tttagtccct gtgagtttag tgaaggggtg actacggggc tatatcctga
     5521 ctaagtcacc ccttcactat gcacatggcc tgtagcctcg cgggttaaag aataattctg
     5581 aacaacactg caaaacaaaa aaaaaaaaaa acgaaccgcg caagtttttt atccatattt
     5641 tttaaaaata ttttttagcg ccgtagattg tttggttgac aggggcaatg cagggcagtc
     5701 gcccaattta tcgccgcgca tgaacttacc tcctcatctc ccccgccagc gccgccaaaa
     5761 acttacctat cggcgtagtg gacatcagcg ccagttcctg cggcagcgtc gtttccgcgt
     5821 tcaaaaaagc cagcaggcgc gggctgggat tgcgttcaaa caattgccga aacagctcgt
     5881 cgccggggtg cagcccacgc tccaacacat gcagcatcag cgtgtcgtag cggcgcgagc
     5941 ggcggcgcgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgggtatcgc cgccggccat
     6061 gccgatgttg acaatgcgcc cctcctgccg gggaaaatca aaatccgtca tcggaatatt
     6121 gccctgctcg gtttccgtca cttcatagcc tccggcctgc ggcaggtgct cgcgcatgta
     6181 gcgggccaaa atctcgtcgt agcccgccgc ctccaaatgc tgattggaaa aaatagccac
     6241 ctccaccaga gcctccttag tcgaaaacgg caagacgtac aagaaccgaa actcgccctc
     6301 ctgtggcgta cggaagtcca tcatcgtagc cactccggga tcgaaaacat cgctttcagc
     6361 gcgcacaaac cagccgcgaa aatgctggtc caagtagttc agtttttgtt tgtcgggagc
     6421 cggcccgcga tacacgctgt tgatgcacca atcggcgctg tacagccatc cgccggcctt
     6481 cacttccaca cccgcgcccg tattgcgcag ttcctccacc agttcatgcc gccactccac
     6541 attcgggaaa ccggccaata ccgccttcaa ctctttgtaa aaatcaatgc cgcgaatgac
     6601 cttgtacaca taaggattta tgtccaacag tctcgaaaaa tcgccctgat gaaaccacag
     6661 ccgccgccat tgatgatgca caatatgctc aaaagggcca ggctcgcgct cccaaaaaca
     6721 ccaggtgcgg tcgttgcggt ctttggtgtc tggttctaat atcagcaccc gccgg
//
LOCUS       DAORLF010000265         6771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6079_length_6771_cov_2.326980, whole genome shotgun sequence.
ACCESSION   DAORLF010000265 DAORLF010000000
VERSION     DAORLF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_6771_cov_2.326980"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK198_18115"
     CDS             <1..1078
                     /locus_tag="PK198_18115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40715.1"
                     /translation="GGDYTVTPVLDANYLNGVSTFDLVLISRHILGVQLLDSPYKMIA
                     ADVNNSRSITTLDLIQLRRLILAIDTEFANNTSWRFVPASYSFPVPTNPWFEQFPEVI
                     NINDLVGSVVNQNFRGVKIGDVNGSVVPNLTDVEDRTMNGEFALNVMDQQVKAGNEYV
                     VTFTGEQMESIRGYQATLTFGNALELVDVVYGAAKEENFGMRFVGEGAITMSWNGEAK
                     SSDNLFSLVFRANAEGRLSEMLGVSSRYTRAEAYNVNSNLMGVALNFGGALVSEGFEL
                     YQNQPNPFKGQTVIGYNLPEAAYVTLTVQDAAGKVLRVVRNEGVKGYNQVALNSNELP
                     ASGVLYYTIATEGFTATKKMIVVQ"
     gene            1175..1696
                     /locus_tag="PK198_18120"
     CDS             1175..1696
                     /locus_tag="PK198_18120"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRF40716.1"
                     /translation="MSAQTRTRKKKDTMQRRSWLLLLLFIPLIAGIIFSILPKPSPKM
                     SGPAFIKQGELKFISKDGKKIISQIDIELAANDEKRTQGLMWRRKMEENQGMLFVMEQ
                     QEMQSFWMLNTYIPLDILFVDELRQIVTIRSNTKPQSLDPVASDKPALYVVEVNAGYC
                     RKFGISVGDKIEF"
     gene            complement(1778..3238)
                     /locus_tag="PK198_18125"
     CDS             complement(1778..3238)
                     /locus_tag="PK198_18125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF40717.1"
                     /translation="MTKKFTFTAVLLPLLCLGLHAQESTKATNSATPKNMWEIGLHGG
                     HLFVSGDVPYKPGFGGGFHIRKATDYVFSLRADFLGGVAHGEGVGPSYDPDRRFDNVW
                     FSGTGLAVISLSNLRWDKSVRKTNLFVMAGGGINYFRTEYMEWDGGSEPEPRMPSIRD
                     WQFSPHAAIGAGLSFRMGPKANIGIEHSAMMGFGRRADYVDGVELEAGIRSPFRDIIH
                     YTSLRLNFNIGNSSTLSEPLYWLNPLDMVLNDISNIKSQQEAFLQDSDGDGVVDALDE
                     EPDTPVNAVVDTKGRVLDSDRDGIPDHVDKEPYYTPRQGETVNSEGVVINPVAAGGVT
                     ESRVKELIDQALQSYNPSASGAAVAEWFLPMLHFANDSYTIKFSDYGNLAGIARMMKS
                     NPKLRLVVTGFTDQTSTEEHNLRLSYLRAKEVVDHLVNNHAIARGRLIIQYKGMEDAL
                     VPAASSYLNRRVEFRSAQADDVEMDPPANVKTGDGY"
     gene            complement(3520..4755)
                     /locus_tag="PK198_18130"
     CDS             complement(3520..4755)
                     /locus_tag="PK198_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRF40718.1"
                     /translation="MKNLLQEYKAQRPEIIFEWADPETGAEGWIVINSLRNGAAGGGT
                     RMRAGLTRDEVVSLAKVMEVKFSVAGPAIGGAKSGINFDPGDPRRDLVLKRWYKAALP
                     ILKHYYGTGGDLNVDELKDVVPITEDLGLWHPQEGIVNGHFKTSDGERIRIIGQLRYG
                     CSKIVEDARYVPDSPQKYAVADLITGYGVAESVRHYYSIFKNTDLKNKRAIIQGWGNV
                     ASAAACFLANEGVRVVGIIDRDGGILHPEGLTLNEVKHLFDTKKGNKLSSPDLIPFAE
                     VNDRIWDAGAEIFIPGAASKLLSEHQVQRLISGGLEVMACGANVPFTDDDVFFGPTAN
                     RTDAQIALIPDFIANCGMARVFAYLMQPEVELTDEAIFQDVSNIIRAALAEVYAHEPS
                     GKYIAGKALELALGKLMNQ"
     gene            4834..5919
                     /locus_tag="PK198_18135"
     CDS             4834..5919
                     /locus_tag="PK198_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40719.1"
                     /translation="MLHHWIRPVGQKSILGDIGEGAPVMGRTVSFYDEDTADLNGVHV
                     ALVGIGDEEADAVRRSLYRMSCPFEGLKITDMGNLRKPDAEFAVAPIRELIDSKIFPV
                     LIGRDVSAMTAQYKAFLSLQQWINLVLADEKLKSEEPYLRDVLHRKEAKLFHFGLLGC
                     QAHFMLPGDWMLLEERHYDVVRLGAARANPEAMEPVVRDADLMAFHVNALRHSDAPGQ
                     EEPSPGGFTMEEACQISRYAGMSDKLKSFGIFGYHQEWDVRGQTAQVIAQMIWYCLEG
                     FYQRKGDFPASTSGLTEYVVDMKDFDGPLVFWKSEKSGRWWFQLAVKAGRKPQRHRLI
                     PCSYEDYRMACRNELPDRLIYALRRFG"
     assembly_gap    6037..6093
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6094..>6470)
                     /locus_tag="PK198_18140"
     CDS             complement(<6094..>6470)
                     /locus_tag="PK198_18140"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRF40720.1"
                     /translation="EQHAQSQLIGAYNFGNIATAIAVGLYFGVSASDIKSAIERYTPE
                     NMRSQVVRKGGNTIILDAYNANPSSTRQALLTFGAMEAQNKIAILGDMLELGEAAREE
                     HQAIAELAQELHLSQIILVGAHY"
     assembly_gap    6471..6551
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6552..>6771)
                     /locus_tag="PK198_18145"
     CDS             complement(<6552..>6771)
                     /locus_tag="PK198_18145"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF40721.1"
                     /translation="IAEPDFGLITNIGKAHLEGFGGIEGVKKGKSELYRFLAERQGVA
                     FINSDEPFLEELAQALDRKFFYGRKPNAN"
BASE COUNT         1618 a         1522 c         1813 g         1680 t
ORIGIN      
        1 aggtggcgac tacacggtaa caccggtgtt ggatgccaac tacctcaacg gcgtgtccac
       61 gtttgacctg gtattgatca gccgccacat tttgggtgtg cagttgttgg acagcccgta
      121 caagatgatc gcagcggacg tgaacaactc acgttcgatc acgacgcttg acctgatcca
      181 attgcgtcgc ctgattttgg ccattgacac ggaatttgcc aacaacacca gctggcgttt
      241 tgtcccggca agctacagct tcccggtacc gaccaacccg tggtttgagc agttccccga
      301 agtgatcaac atcaacgacc tcgtgggtag cgtggttaac cagaacttcc gcggcgtgaa
      361 aatcggcgac gtgaacggtt cagtagtacc gaacctgacg gatgttgaag accgtacgat
      421 gaacggcgaa ttcgctctca acgtaatgga tcagcaggtg aaggcaggca acgagtacgt
      481 tgtaacgttc acaggcgagc agatggaaag catccgtggc taccaggcta cgctgacctt
      541 cggcaacgcg ctggaattgg tggacgtagt ttacggagca gctaaagaag aaaactttgg
      601 catgcgtttc gtaggcgaag gcgccatcac gatgagctgg aacggcgaag cgaagtcttc
      661 cgacaacctg ttcagtctgg tattccgcgc caatgcggaa ggccgtctga gcgagatgtt
      721 gggtgtgagc agccgttaca cgcgtgcaga agcatacaac gtgaacagca acctgatggg
      781 cgtagcgctg aacttcggcg gtgcattggt gagcgaaggc tttgagttgt accagaacca
      841 gccgaacccc ttcaagggtc agacggtgat cggatacaac ctgccggaag cagcttatgt
      901 aacgttgacg gtacaggacg cagccggtaa ggtactgcgc gtggtacgca acgaaggtgt
      961 gaagggttac aaccaagtgg ctctgaacag caacgaactg cctgcatcgg gtgtattgta
     1021 ctacacgatt gcaacggaag gctttactgc aacgaagaag atgatcgtcg ttcagtaatt
     1081 cctattcctt aggataaaat gagataaggg gttgaaggct tgggctttca gcccctttct
     1141 ctttttgttt acttttgcat taaaatccag agttatgtct gcacaaacgc gtactcgtaa
     1201 aaagaaagat accatgcaac gccgttcctg gctgctgctg ttgcttttca tcccgctgat
     1261 agcgggcatc attttttcaa ttctgcctaa accatcgccc aaaatgtcag gacctgcatt
     1321 tattaagcaa ggggaattaa aattcatcag taaagacggt aagaagatca tttctcaaat
     1381 agatattgag ttggccgcca atgatgagaa acgaacgcag ggtttgatgt ggcgtcgaaa
     1441 aatggaggaa aatcagggca tgctttttgt aatggagcaa caggaaatgc aatcgttttg
     1501 gatgctgaat acctatatcc ctctggatat tctatttgtg gatgaactgc gacaaattgt
     1561 gacgattaga agcaatacca aaccgcagtc attagacccg gttgcatcag ataaacctgc
     1621 attgtatgtg gtggaggtga atgcagggta ctgccgaaaa tttggtattt ccgtcggcga
     1681 taagattgaa ttttgatgta gtcttgagag gatgaccaaa taaaaaagcc gttgccccca
     1741 cgagcaacgg ctttttcata tcaaccgaaa aaagaaatta ataaccgtct cctgttttta
     1801 cgtttgccgg aggatccatt tcgacatcgt ctgcctgagc agagcggaac tctactctgc
     1861 ggttgagata gctggaagcg gcaggaacca aggcgtcctc catgcctttg tactgaataa
     1921 tcaggcgccc gcgagcgatg gcatgattat tgaccaaatg atctactacc tccttggcac
     1981 ggagatagga caggcgtaag ttatgctcct ctgtggaggt ttgatcggta aaaccagtaa
     2041 ccaccaatct gagttttgga ttggatttca tcatgcgggc aatacctgcc aaattgccgt
     2101 aatctgaaaa cttgatggtg tagctgtcat tggcgaaatg cagcataggc aagaaccatt
     2161 cagcaacagc tgcgccggaa gcgcttgggt tataggattg gagcgcttgg tcaatgagtt
     2221 cttttactct ggattccgtg acgcctccgg cagctaccgg gttgatgacg actccctcgc
     2281 tgttcacggt ttccccctga cgaggcgtat aataaggctc cttatcaacg tggtcgggaa
     2341 taccgtctct gtcgctgtcc aagacgcggc cttttgtatc cacaacggcg ttgaccggag
     2401 tatcgggttc ttcgtcaagg gcatccacga cgccgtcacc gtcagaatcc tgcaggaaag
     2461 cctcctgttg cgatttgatg ttggaaatgt cattgagtac catatctaaa ggattgagcc
     2521 aatagagcgg ctcagacaga gtggaagaat ttcctatgtt gaagttgagg cgcaggctgg
     2581 tgtaatgaat aatgtcgcgg aagggggaac gaatgcctgc ctccagttca acaccgtcca
     2641 cataatcggc acgacgacca aagcccatca tggcggagtg ttcgatgccg atgttggcct
     2701 taggacccat gcggaaggac aatcctgcac cgatagctgc atgaggagag aattgccaat
     2761 cgcgaatgct cggcatgcgc ggttctggtt ccgagccgcc atcccattcc atatactctg
     2821 tacggaaata attgataccc cctccggcca tgacaaagag attggttttc ctgacggact
     2881 tatcccagcg aaggttgctg aggctgatga cggccaagcc ggtgccggaa aaccagacat
     2941 tgtcgaagcg acggtccggg tcataactag gtcctactcc ttctccgtgc gcaacgccgc
     3001 caaggaagtc ggcgcgtaag gagaaaacgt agtcggttgc cttgcggatg tgaaatccgc
     3061 ctccaaatcc aggcttgtaa ggaacatcgc cggagacgaa taggtggcct ccgtgtaagc
     3121 cgatttccca catgttcttg ggagtggcgc tgttggttgc tttagtgctt tcctgagcat
     3181 gaagccccag gcacagaagc ggcagtagta ctgctgtgaa agtgaatttt ttagtcataa
     3241 ttgtagggat tactgtgggg aaaaaaaaat cggtttttct catagtaaat cggtttcaag
     3301 gccaagttgg aatacttgtt cggttgatga agcaaaaata ttgatatttt ccagacgaag
     3361 caagttttca ggcacgcaac actacaattg ctgaaaatat atgcacaatg tttatacgat
     3421 aaaaaatgca aaaggataca aaatgcctgt ttgtgcttcc gaaaaacata aaaatgctgc
     3481 aatgagaagt gcgctcgttg gtagtgcggc gggtacttct tattggttca tcagcttacc
     3541 aagcgccagt tccaatgctt ttccggctat gtatttgccc gaaggctcat gcgcatacac
     3601 ttctgccaat gcagctctga taatattgga gacatcctgg aagatggcct catcggttag
     3661 ttctacttcg ggctgcatga ggtaggcaaa tacgcgggcc atgccgcagt tggcaataaa
     3721 atccgggata agtgcaattt gtgcatccgt tcggttggca gtaggcccga aaaaaacatc
     3781 atcatcggta aacggaacat tggcgccgca agccattact tcgaggccgc cggaaatcaa
     3841 acgctgcacc tgatgttcgg aaagcaattt ggaagctgcg ccgggaatga aaatttcagc
     3901 gccggcatcc cagatgcggt cgttgacttc tgcaaaaggg atgagatcgg gcgagctgag
     3961 cttgttgcct tttttggtgt cgaagaggtg ttttacttca ttcaaagtaa ggccttccgg
     4021 atgaagaata cctccgtcgc gatcaatgat gccgactacg cgcacgcctt cattggcaag
     4081 aaagcaagcg gcagcagacg ccacgttgcc ccagccctgg atgatggccc gtttgttttt
     4141 taaatctgta ttcttaaaaa tggaataata atgccggact gattccgcca ctccatagcc
     4201 tgtaatgaga tcggcgacgg catatttttg ggggctgtcg ggcacatacc tggcatcctc
     4261 cacgatttta gagcagccat agcgaagttg ccctataatg cggatgcgct cgccgtcgga
     4321 ggttttgaaa tggccattta cgatgccttc ctgtggatgc cagaggccga ggtcttcggt
     4381 aatgggcact acgtctttga gttcatccac gttgaggtcg ccccctgtgc cgtagtaatg
     4441 tttgagaata ggaagggcgg ctttgtacca gcgctttaga accaggtctc ggcgcgggtc
     4501 tccgggatca aagttgatgc ccgatttggc gccgcctatg gcagggcctg ccaccgaaaa
     4561 ttttacttcc atcactttgg caagcgaaac cacctcgtca cgggtgaggc ctgcgcgcat
     4621 acgggtgccg ccgccggccg cgccattgcg caaagagttg atgacgatcc agccctcggc
     4681 gccggtttcg gggtcggccc attcaaaaat aatttccggg cgttgagctt tgtattcctg
     4741 cagcaggttt ttcattttgt aatttggttt tgatttggcg gcgcaaaagt aggtaagtat
     4801 tgactacttt tggcggtttc taaaaagtag tttatgttac atcattggat tcggcctgtt
     4861 ggtcagaaaa gtatattggg agatattggt gaaggtgctc cggtaatggg gcgcactgtt
     4921 tcgttttacg atgaagacac cgccgatctc aacggcgtgc atgtggcttt ggtgggaata
     4981 ggcgatgagg aggccgacgc ggtgagaagg tctttgtata gaatgagctg tcctttcgag
     5041 ggattaaaaa ttacagacat gggtaatttg cgcaaaccgg atgccgagtt tgcagtggcg
     5101 cccatcagag agttgattga cagcaaaatt tttcctgtgt tgataggacg tgacgtatcg
     5161 gcgatgacgg cccagtacaa ggcttttttg agcctgcaac aatggatcaa tcttgtgttg
     5221 gcggatgaaa agctgaagtc ggaggaacct tatttgcgcg atgtcttgca ccgaaaagag
     5281 gcaaaacttt ttcattttgg ccttttgggt tgccaggctc attttatgct gccgggtgat
     5341 tggatgctgt tggaggagag gcattacgat gtggtgcgcc tgggggcggc gcgtgccaat
     5401 cctgaagcta tggaaccggt ggtgcgcgat gctgatttga tggcttttca tgtcaacgcg
     5461 ttgcgccatt cggatgcgcc cggccaggag gagccttctc cggggggctt cacgatggaa
     5521 gaggcttgcc agatcagccg ttatgcgggt atgagtgata aactaaaatc cttcggtatt
     5581 tttggatacc atcaggagtg ggatgtgcgg ggccagacgg cgcaggtgat agcccagatg
     5641 atctggtatt gtttggaggg tttttatcaa cggaagggag attttccggc ttcaaccagc
     5701 ggattgacag agtatgtggt ggatatgaag gattttgacg ggcctttggt gttttggaaa
     5761 agcgaaaaaa gcggtcgctg gtggttccaa ttagcggtga aagcagggcg caaaccccag
     5821 cggcaccggc tgattccatg ctcttatgaa gattaccgga tggcttgtag aaacgaactg
     5881 cctgaccgcc tgatttatgc tctgcggagg tttggataaa acgttacgct tcttttaagg
     5941 cctctactat ggtctccaac tttatgcctc tggagccttt tatcagaaaa tgagtactgg
     6001 aaaacgccct ctccaatagg tgttttcgca atgcctnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtaatgc gctccaacca atatgatctg
     6121 tgaaaggtgc aactcttgcg ccagttcggc tatggcctga tgctcttccc gggccgcctc
     6181 ccccagctcc agcatgtctc ctaaaatggc aattttgttt tgggcttcca tagctccgaa
     6241 agtcagcagg gcttgtcggg tgctgctcgg attggcatta taagcatcca aaatgatggt
     6301 attgccgcct ttgcgaacca cctgtgaacg catgttttcg ggcgtgtatc gctcaatcgc
     6361 ggactttatg tccgatgcgc tcactccaaa gtacagccct acggctatgg cagtggcgat
     6421 attgccgaag ttgtaggcgc cgatgagttg gctttgagca tgttgttcgt nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn naatttgcgt ttggctttcg tccataaaaa aacttgcggt caagagcttg
     6601 tgcaagctct tccagaaacg gctcgtcgct attgataaag gctactccct ggcgctcggc
     6661 taagaagcgg tagagttcgg attttccttt tttgacgcct tcaatgcctc caaaaccttc
     6721 caaatgcgct tttccgatat tggtaatcag gccaaaatcg ggttcggcta t
//
LOCUS       DAORLF010000266         6755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6101_length_6755_cov_2.009403, whole genome shotgun sequence.
ACCESSION   DAORLF010000266 DAORLF010000000
VERSION     DAORLF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_6755_cov_2.009403"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>497
                     /locus_tag="PK198_18150"
     CDS             <1..>497
                     /locus_tag="PK198_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40722.1"
                     /translation="TVMLQACPGQTVTYNGTTLTAGEVRSFTLVNAAGCDSTVTVTVV
                     EFPALQVQATAVESCPNRPSGSITVAATGQGPFSYRINGGAAQAEALFGNLASGTYNL
                     LVQDGNGCSAESRATVTGLAALQAQVLNAELSCDADSVMVEAVIASGDDGQVQLLWDD
                     GSTAA"
     assembly_gap    498..542
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <543..899
                     /locus_tag="PK198_18155"
     CDS             <543..899
                     /locus_tag="PK198_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40723.1"
                     /translation="VINSCDTLRQTVQVRYTERPDLTPIFVPNAFSPNDDGINDLFRA
                     FASTEVRVLSFDLMVADRWGNLMFRTQNPAEGWNGQLRERQMDPAVFVWHLKATVQHC
                     GQIMELNEMGDVTLVR"
     gene            complement(943..1398)
                     /locus_tag="PK198_18160"
     CDS             complement(943..1398)
                     /locus_tag="PK198_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40724.1"
                     /translation="MRTTSLHIPTLILMLCAAALPAQTVQKTLVRAFDKGDAQELTLD
                     LGYPATIQHWDAATVRVQMQIGLDNVHESMLQTLISGGRYNLKGEDQGQAFAISAPGL
                     RKNVRIGEKLLTERVAFSVFVPQGLTVRIVETKTTEGVVEGISALKPMR"
     assembly_gap    1703..1727
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1728..>2079
                     /locus_tag="PK198_18165"
     CDS             <1728..>2079
                     /locus_tag="PK198_18165"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40725.1"
                     /translation="NTTPTIPTEEDYGPSREKWGFMDRNGRLSIKGNYDEVRGFVQGR
                     AAVRKKGRWGYIDNRARWIAEPQFRAAWSFSEGLARVTTFEDKAGFIDTNGDWAIQPT
                     YADAGDFRFGRARAM"
     assembly_gap    2080..2093
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2094..2813
                     /locus_tag="PK198_18170"
     CDS             <2094..2813
                     /locus_tag="PK198_18170"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40726.1"
                     /translation="YGYIDTQGRWVIPARYEEAAPAFKNGYAKVGLSELYGLIDTNGT
                     LVLPLVFDQVFPFSPEGLARAKKDEAYRFYLLNGKPAFDAKFLFAGDFEEGLAPAQNE
                     AGFGLIDTKGKWQVQPGFDRIWYAGEGRWGVEQDGLFGIIDGEGNEIVAPQFPQLYGF
                     TEGLAPYQWNELWGYLDREGRIVTEPIFGLAWGFNEGLARVAVYGGIAFINSEGFQTL
                     PAKYIDARDFSEGLAPVQVRR"
     gene            complement(3084..4658)
                     /locus_tag="PK198_18175"
     CDS             complement(3084..4658)
                     /locus_tag="PK198_18175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HRF40727.1"
                     /translation="MKTTILFIAMLFSGLTCLAQQTISGYVYEDLNVNNKKDKREKGI
                     AGVSVTNGVDVVQTDALGRYTLPLQADQILAVIKPADYDLPKNVLHLPQFYHIHKPVG
                     APELKYKGVAPTGELPEEINFALLPAKVEATFTALVFGDPQPYNLMEMAFFEKGIVHE
                     VEGIANVSLGISLGDLVGDDLSLFKSYQEAVAKVGIPWYNVMGNHDLNLDVTRDELAD
                     ETFEAHFGPSHYAFNYGKVHFIVLDDILYPDPRDGTGYWGGFREDQLAFIKNDLAFVP
                     KDRLIVLAMHIPLFDEDYGDSFRDSDRTALFELLEEFPHTLSLSAHTHLQRQYFFDED
                     DGWKQPKPHHHYNVGTTSGDWYKGKLNKNGVPVSTMRDGTPKGYAFIDFDGNSYALRY
                     KVANEEPSYQMEIFAPKVIEKDKRTSAGIYVNFFIGNEKSTLRFRIDEGTWQNMHYLE
                     EYDPAYLLEMFDWDTTTELMDGKRPSNPEKCRHLWRANIPNRLPVGKHVIEVEAVDMF
                     GKRYTGQTHYEIKTAE"
     gene            4818..>5539
                     /locus_tag="PK198_18180"
     CDS             4818..>5539
                     /locus_tag="PK198_18180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF40728.1"
                     /translation="MFQLTGLPPFSDYELADSGNFRKLERFGAYYLSRPEPQAIWKPA
                     LSDAEWQGLAHATFERRGGKGNDSGDEGNWQLRSGMPEQWHISYRHEALQLKLRLGLT
                     SFKHVGVFPEQADNWNYIYDAVSGMSPAPAVLNLFAYTGAASLAARAAGAEVVHVDAV
                     KPVITWARQNMEASGLDNIRWVVEDALKFAQREDRRGRRYQGIILDPPAYGRGPDGEK
                     WLLEKHLGELLEICCRLLDPEQ"
     assembly_gap    5540..5637
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5758..>6755
                     /locus_tag="PK198_18185"
     CDS             5758..>6755
                     /locus_tag="PK198_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRF40729.1"
                     /translation="MTLQERLSALSELGRRLQLPDDYLDAVIHRTAFNNGWFTPENQR
                     KAIQAIATRMLDADKLHAWAGRYFLPDAPALKTVGLVMAGNIPLVGWHDVQCVFTAGH
                     RAQIKLSDKDQYLLPCLLRMLEQIDARTAAYFEMVHQLRDFDAVIATGSNNTSRYFES
                     YFAHVPHIIRRNRNAVAVLDGSESAEALAALGDDVFRFFGLGCRNVSKIYVPQDYDFM
                     PLLNALHEYREIVLHDKYKNNFDYNFALYTLNKTPFYMAGSVMLTENAGFSSRIAALH
                     YERYDKVAALEQQLSAHRDAIQCVIGRPGLLAHTEVIAFGRAQAPDGSCGRGRPRSA"
BASE COUNT         1459 a         1692 c         1831 g         1591 t
ORIGIN      
        1 gcacggtgat gctccaggct tgtcccggcc aaacagtgac ttacaacgga accacgctga
       61 cggcgggcga ggtgcgcagt ttcacgttgg tcaatgcggc aggttgtgat tctacggtga
      121 cggtgacggt ggtggaattc cccgctttgc aagtacaggc tacggcagtg gagagttgcc
      181 ccaatcgccc atcggggagc atcacggttg cagccacggg gcaagggccg ttcagctatc
      241 gcatcaatgg cggcgcggcg caggcagagg cccttttcgg gaatcttgct tcaggaacgt
      301 acaatctgct ggtgcaggat gggaatggtt gcagtgccga atcgcgggct acggtgacgg
      361 gtttggcagc cttgcaggcg caggttttga acgccgaact ctcctgcgat gccgattctg
      421 tgatggtgga agctgtcatt gcatcgggcg acgacgggca ggtgcaactc ctgtgggacg
      481 atggctcgac agcggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nngtcatcaa ttcctgcgat accctgcgcc aaacggtgca ggtgcgctac accgaacggc
      601 cagatttgac gccgatattt gtacccaatg ccttcagtcc caacgatgac ggcatcaacg
      661 acttgttccg ggccttcgct tctacggaag tgcgggtact cagtttcgac ctgatggtag
      721 ccgaccgctg gggcaatctc atgttcagaa cccaaaatcc ggcagagggc tggaacgggc
      781 aattgcgcga gcgccagatg gacccggcag tgttcgtgtg gcacctcaaa gctaccgtgc
      841 agcactgcgg ccagatcatg gaactgaatg aaatgggcga tgtgacgctg gtgcggtagg
      901 cggaattgcg ataaggatgt gccgaccgca caaaaccgag aatcacctca ttggcttgag
      961 ggcagaaatg ccttccacaa cgccttccgt cgttttggtt tctacaatcc tgacggtcag
     1021 tccttgcgga acaaacacag aaaaggcaac gcgctcggtg agcagctttt cgccgatgcg
     1081 tacgtttttg cgcaagccgg gcgcactgat ggcaaatgcc tggccctgat cttctccttt
     1141 cagattgtaa cggccaccgg aaatcaaggt ttggagcatg ctttcgtgta cattgtccag
     1201 accgatttgc atttgtaccc gcacggtggc ggcgtcccaa tgctgaatgg ttgcaggata
     1261 acccagatca agcgtcaact cctgcgcatc gcctttgtcg aaggcccgca cgagcgtttt
     1321 ctgaaccgtc tgggcaggaa gcgcagcggc gcaaagcatc agtatgaggg tgggaatgtg
     1381 tagagatgtt gtcctcatag gtttcaaaat ttaataaatg agcaaaaacc atctgaaatc
     1441 aattcgggca taagttacaa cttttttggg gaaaagtcaa tttttatttt gaaatattgt
     1501 cggtaactgt cgttaataac aaattttcat ttttggatca tcgcaaaagc agcaaatgtt
     1561 ggtcgcagca cgtttttcaa actaccataa acactgcatt tctatgccct tttcgatttc
     1621 gttccatcat catcgtctgc tgtacccggt gctattcagc gtggtcgctt tgttcgcctc
     1681 ttcgtgccgg agtgattcgg ctnnnnnnnn nnnnnnnnnn nnnnnnnaac acaaccccga
     1741 ccattcccac cgaggaagac tacggcccct cgcgggaaaa atggggtttt atggatcgca
     1801 acgggcgcct gtccatcaag ggcaattacg acgaggtgcg cggttttgtg cagggccgtg
     1861 ccgctgtgcg caaaaaaggg cgctggggct acattgataa ccgcgcccgg tggattgcag
     1921 agcctcagtt tcgggcggcc tggtcgtttt cggaagggct ggcccgcgtg acgacttttg
     1981 aagacaaagc cggatttatt gatacaaacg gggactgggc catacagccg acttatgccg
     2041 atgccggaga ttttcgtttc ggacgtgcgc gcgctatggn nnnnnnnnnn nnntacggat
     2101 atattgatac acaaggccgt tgggtcattc cggcccggta cgaggaggct gcgccggctt
     2161 tcaaaaacgg ctatgcaaaa gtggggctga gcgagttgta cggcctcatt gacaccaatg
     2221 gaacgctggt gctgcctttg gttttcgacc aggtttttcc tttttcgccg gagggcctgg
     2281 cccgcgctaa aaaagacgaa gcctaccggt tttatctcct gaacgggaag cctgcatttg
     2341 atgcaaagtt tttgtttgcc ggagattttg aggaaggcct ggcgccggcg cagaacgaag
     2401 caggcttcgg actcatagat accaaaggaa aatggcaggt gcagccgggc ttcgacagaa
     2461 tatggtatgc cggcgagggg cgttggggcg tggagcagga cggtcttttc ggcattattg
     2521 acggggaggg caatgaaatc gttgcgccgc aatttcctca gttgtacggc tttaccgagg
     2581 gcctggcccc ataccaatgg aatgaattgt gggggtattt agaccgcgag ggccgcatcg
     2641 tcacagagcc gattttcgga ctggcctggg gtttcaacga aggtctggcg cgggtggctg
     2701 tttacggggg catcgctttc atcaatagcg agggcttcca aacgctgccg gcaaaataca
     2761 tagacgcccg cgatttttca gaaggtctgg cgccggtgca ggtgcgaaga tagtggaaat
     2821 gggcagggtg gggttagcga ctgtgattct cattccagtc gagtttggtt attttacccc
     2881 gacttacaat catcaccgaa acctccctgt ccaaggctgt accataaaaa tcaaaatatg
     2941 aaagcatcaa tatatacaac cctcgtaaag tcatagccgg taatgtgtgg tttgctgtct
     3001 tagattagcc ggaaatagaa taattgccca cttcatggtt ttatctggta tgctattcct
     3061 gttcgggcga tcttttagcg ctactattcg gctgttttta tttcgtaatg cgtttgaccg
     3121 gtatagcgct tgccaaacat gtcaacggcc tccacttcta tgacgtgttt acccaccggc
     3181 aacctgttgg ggatattggc ccgccagagg tgtctgcatt tttcaggatt ggaggggcgt
     3241 ttcccgtcca ttagttcagt ggtggtatcc caatcaaaca tttctaaaag gtaggccggg
     3301 tcatattctt ccaaatagtg catgttttgc caggttccct catctatacg aaaacgcagg
     3361 gtggattttt cattgccgat aaaaaagttg acatatatcc ccgccgaagt tcgcttatct
     3421 ttttcgatca ctttgggggc aaaaatctcc atttgatacg aaggctcctc attggcgact
     3481 ttgtagcgca aggcatagct gttgccatcg aaatctatga aagcataccc tttgggagta
     3541 ccgtctcgca tggtagaaac gggcacgcca tttttgttca gttttccctt gtaccagtcg
     3601 ccggaggtgg tgcccacatt gtagtggtgg tggggcttgg gctgtttcca cccgtcatcc
     3661 tcatcgaaga agtactggcg ctgtaaatgg gtgtgggccg acagcgaaag cgtgtgcgga
     3721 aattcctcca gcaattcaaa aagcgccgtt ctgtcgctgt cgcggaatga atcgccatag
     3781 tcttcatcga agagcggtat gtgcatggcc aatacgatca ggcgatcttt gggcacaaaa
     3841 gccagatcgt ttttgataaa agccaactga tcttccctga aacctcccca ataacccgtt
     3901 ccatcccgcg gatcagggta gagaatgtcg tccagaacga taaaatgcac ctttccgtaa
     3961 ttgaaggcgt agtgagatgg gccaaaatgc gcctcgaagg tttcatcggc aagttcgtct
     4021 cgtgttacat ccagattcag atcgtgattg cccatgacat tgtaccaggg tatgcccact
     4081 ttggcgacgg cctcctgata ggatttgaaa aggctcaggt cgtcgcccac gaggtcaccc
     4141 agcgatattc ccaaactgac attcgcgatc ccctcgactt catggactat tcctttttca
     4201 aaaaaggcca tttccatcaa attgtagggc tgaggatcgc caaacaccag agccgtaaaa
     4261 gtagcttcca ctttggccgg cagcaaagca aagttgattt cttcgggcag ttcgccggta
     4321 ggggccacgc ctttgtattt caattcaggg gcgcccaccg gtttgtgaat gtgataaaac
     4381 tgcggcaggt ggagtacgtt tttgggcaag tcataatcgg caggtttaat caccgccagt
     4441 atctgatctg cttgaagggg cagggtatag cgccccaaag catccgtctg aaccacatcc
     4501 acgccattgg taaccgatac cccggcaatg cctttttccc gcttgtcttt cttgttgtta
     4561 acattgaggt cttcatagac atatcctgat atggtctgtt gtgcgaggca ggtcagcccg
     4621 ctgaacaaca tggctatgaa cagtatcgtg gttttcatac gcattggttt tcaattgaat
     4681 cgtaaaaata aggttgatct tcatcataca gattaaactt gccttaattt ttcgtgattt
     4741 tcgcgtggcg gacgaatggt cgtgcatacc gcccgccgct cgttgcgaaa tccatcctga
     4801 aaccctacct ttgcggcatg tttcaactca caggtcttcc gccgttttcg gattacgaac
     4861 tcgccgactc cggcaacttc agaaaattgg agcgattcgg agcgtactac ctttcccggc
     4921 ccgaaccaca ggccatctgg aaaccggccc tgtcggacgc agaatggcaa ggcctggcgc
     4981 acgccacgtt tgagcggcgc ggcggcaaag gcaacgattc cggcgacgag ggcaactggc
     5041 aactgcgctc cggcatgccc gaacaatggc atatttcgta ccggcacgag gcgctccaac
     5101 tcaagctgcg gctggggctg acctccttca agcacgttgg cgtgttcccg gaacaagccg
     5161 ataactggaa ctatatttac gatgcggttt ccggcatgtc gccggcgccg gcggtgctca
     5221 atctttttgc ttacacaggc gccgcttctt tggctgcccg ggctgccggc gcagaggtcg
     5281 tgcatgtgga tgcagtgaaa cccgtcatca cctgggcacg gcaaaacatg gaggcctccg
     5341 gtttggacaa tatccgctgg gtggtggaag acgcgctcaa gtttgcccaa cgcgaagacc
     5401 gtcgcggacg ccgctatcag ggcatcatcc tcgatccgcc ggcttacggg cgcggccccg
     5461 acggtgagaa atggctgttg gagaagcatt tgggggaact gttggagatt tgctgccgcc
     5521 tgttggaccc cgaacagcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca
     5641 atacgaattc ggcgaactgt tcattccttc cactgccggg ccgaggctgc ctttgggggt
     5701 gtttttgagg tttcggaggt agaaatttct gaactttggc cccgcaaacg acacaacatg
     5761 accttacaag aacgactatc agcactgagc gaactgggac gccggctgca actgcccgac
     5821 gactatctgg acgctgtgat tcaccgcacg gctttcaata acggctggtt tacgcctgaa
     5881 aatcagcgca aagcgataca ggccatcgcc acgcgtatgc tggatgcgga caagttgcac
     5941 gcttgggcag gccgctattt tttgcccgat gcgcccgccc tcaaaacggt gggcttggtc
     6001 atggctggca atatccctct cgtgggctgg cacgatgtgc agtgcgtgtt tactgcgggg
     6061 caccgcgcac agatcaagct gtcggacaaa gatcagtacc tgctgccttg cctgctgagg
     6121 atgttggaac agatagatgc ccgcacggca gcttactttg agatggtgca tcagttgcgg
     6181 gatttcgacg ccgtcatcgc tacgggcagc aacaacacct cgcgctattt cgagtcttat
     6241 ttcgcgcatg tgccccacat cattcggcgc aaccggaatg ccgttgccgt tctcgacggt
     6301 tcggaatcgg cagaagcatt ggcggccctc ggcgacgatg tttttcggtt tttcgggctg
     6361 ggctgccgaa atgtttccaa aatatatgtg cctcaggatt atgactttat gcccttgctg
     6421 aatgcgctgc atgaataccg ggaaatcgtt ttgcacgata aatacaaaaa taatttcgac
     6481 tacaattttg cgctctacac cctcaataaa acgccctttt acatggccgg cagcgttatg
     6541 ctcaccgaaa atgcgggctt cagttcgcgc atagcggctc tgcactacga gcgctatgac
     6601 aaggtagcgg cattagagca acaattgtct gcgcaccggg acgccattca gtgcgtgatc
     6661 ggacgccccg gtttattggc tcatacagag gtgattgcct tcgggcgtgc gcaggcaccg
     6721 gacggaagtt gcggacgagg acgtccgcgc tccgc
//
LOCUS       DAORLF010000267         6744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6113_length_6744_cov_2.089999, whole genome shotgun sequence.
ACCESSION   DAORLF010000267 DAORLF010000000
VERSION     DAORLF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6113_length_6744_cov_2.089999"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    373..412
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <413..>689
                     /locus_tag="PK198_18190"
     CDS             <413..>689
                     /locus_tag="PK198_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRF40730.1"
                     /translation="EGTRYKYAGKSPQTGFDCSGFTNYVFKNYGVQLSPSSAEQAKQG
                     RAKPVQEAKPGDLIFFRRSPAEPIFHVALVIANDRTGIHVIHSTTSRG"
     assembly_gap    690..761
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(871..2118)
                     /gene="pepT"
                     /locus_tag="PK198_18195"
     CDS             complement(871..2118)
                     /gene="pepT"
                     /locus_tag="PK198_18195"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRF40731.1"
                     /translation="MSLSFQHQVLDRFLRYVVIDTQSDPESKTIPSTEKQKNLGKLLV
                     KELHEIGISDAKLDKYGYVYATIPANTTKNVPVICYCSHMDTSPDASGAGVKPIVHRN
                     YQGQDLVLPDDPTQVIRAKEHPDLLEQIGNDIVTASGTTLLGADNKAGVAAIMDAAHY
                     LMTHPEWKHGVIKILFTPDEEIGRGVDKVNIKKLGAQFAYTVDGERLGSIEDETFSAD
                     AAVITIEGVSAHPGFAKGKMVSAIKIAAAIIERLPKDRLSPETTEGREGFVHPGLISG
                     SLESATIRFIVRDFTLEGLKKHEDELRAIMESVLAEYPAAKGKMEIIEQYRNMKEVLK
                     DYPQVAAHAVEAIKRAGITPHLSSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWV
                     SVQDMEKAAETLVHLAGVWEEHS"
     gene            complement(2361..2864)
                     /locus_tag="PK198_18200"
     CDS             complement(2361..2864)
                     /locus_tag="PK198_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40732.1"
                     /translation="MKVRDYRRIVGVAWSDAHPDRYINYINTAVGLNQNDFAHVLDEM
                     QTQMNAMQLAIAEVNPNFKPRLYDTGGSMVGAVQERDYSVSPTHKEVLFAGVSSKQYT
                     SQREMLDDARTILKDKSGLDINKIALLDHLFTHPEQASAMGAYYENMKRQFEAVHAGI
                     AKELETK"
     gene            complement(2758..4827)
                     /locus_tag="PK198_18205"
     CDS             complement(2758..4827)
                     /locus_tag="PK198_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40733.1"
                     /translation="MKKHFFIIPAFLLTSLFAFAQSIPQGINYQAVARDSKGAPLALQ
                     NIGLRVSLRAGDAGGKAAYEEVHLLKTNELGMFQLVIGQGRTERGDFRQVPWSEHPIW
                     MVLALDETGKGDFVTLAATQLMAVPYAFHAGTAETWSAPMTELEKNRRCSPTGIPYWT
                     NLGNYNVNDTCHFIGTTVPVDFIFKTNNVERMRITKNGELLIVARTNFQDNVQFSGDS
                     VIVDNDLFVGGNADVGINLSIGNDVHIGNDLQVDSNVQINNYLQVEGPSWLNNRVIVD
                     AYIQGSQSSQDSYPLLIRGSGQGLAIQVDPSNFSPLASGRGNNYISFWNSSATMTGRV
                     EGMSKADLDPTGLLSIVSSIITTPPSTLNMNPASILQLPTLNLFAGSAITGIDFSALS
                     FSSNPFVTDGNLIFNTGAFNPGDFNALINSSLSSPSASFLNIYNGRDLNGTPASILWH
                     VIQNIVCDTTTGLFPLIVGDPEAATNFKSQIFSDYSMDILQGGISTLGSLIQFAASFA
                     SPLDPEDVLSTAIGAAVEITNMVIYGSYADINLGQCGRHLSVWFCRLRRVAGAGKYGR
                     DYASGRRSRSNRRKGVETIRQPGAIHGGLHFAHCAGEYAYRQEPRERPRENCLHGPGT
                     CESKRPGKNRRLHPAFGRERWYGHCCIAQKHEGAGLPPHRGRGLERCPSRQIHQLHQH
                     RRRSQSE"
     assembly_gap    5032..5167
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5168..>5589)
                     /locus_tag="PK198_18210"
     CDS             complement(<5168..>5589)
                     /locus_tag="PK198_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40734.1"
                     /translation="GNKKILVTYPEALFEKVAFMGQIPVKVRGQVNIGDFILPSGFND
                     GTGMAVSPQRISPGQYRQIVGVAWSATPAGSKLAYINMAIGLNNNDIAKLVEEQQNEI
                     EKLNQEVTDLKNDFAALNRRLAALESGKPIAPLSAQPQ"
     assembly_gap    5590..5608
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5609..>6744)
                     /locus_tag="PK198_18215"
     CDS             complement(<5609..>6744)
                     /locus_tag="PK198_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40735.1"
                     /translation="RRCSSTGIPFWSNLGNYNVNDTCHFIGTTVPVDFIFKTNNVERM
                     RITKEGELIILARVTFVDNVQFSGDSVIIDNDLFVGGNVDVGQDLDVHGDAAIDQNLD
                     VGGNGGIGQDLGVGGNGSISGNLEVGGDGRFTNIFVTNNAEIGNDLSIGRDGSIGRDL
                     AVTRNTAVGGTLTSSGKLTVNNVTDLNGQVTIRANVGGGDASYNAYPLRVEGSAQGIA
                     VKVNQGTPNGSNNFITFFDNNNTARGRIEGQTSAEVASSPEFIFETSIYTAEVVAAGV
                     NIGLSALPNACAGVGAVACPPEPSVVAIAIAEEILAIANLAAYEAFAFANVGVTYQSG
                     SADYAEWLKRSHAAETMTPGDIVGVKGGLISKRTDNGVSQYLVI"
BASE COUNT         1432 a         1632 c         1711 g         1702 t
ORIGIN      
        1 accctctact ttcttccaga tagatggatt cagagaggac ttcggcatag atcattatcg
       61 gccggggatg cgggtgctgg acttgtctga tgacggcggt tttatgcacg cggtgaaata
      121 tttggatgta gccggatggt aattcattgc tgccaaagcc attacttttg tactaattca
      181 tcgggcgttt tcgttttgcc ccaaaatttt cccgatgaac acgccaacta tctcctccac
      241 acttcgtatc gccttgcttt gctgcggaat gacactcgtt atcagcgggt gtaattttct
      301 gcgcaatctg acttcggagc cggtaagctc cgatgcgggc acgagcaata cctccaactt
      361 ccgccgcaat atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgaaggaa
      421 cgcgatacaa gtatgccgga aaatcgccgc aaacgggatt cgactgttcg ggattcacca
      481 attatgtttt taaaaactac ggggtacaac tatcgccctc gtctgccgaa caggcgaaac
      541 agggccgggc aaaacctgtg caggaagcca aacccggcga tctgatcttt tttcgcagaa
      601 gccccgcaga acctattttt catgtcgctt tggtcatcgc caacgaccgc accggtatcc
      661 atgtcataca cagcaccacc tcgcgcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctcggtggg cgccgaggct
      781 gatttctgac aatatctgag cgtagtgaag ggctgacaac ggggctgtgt tcctgctcag
      841 tcaccccttc acttttcttc ggggcatcaa ctaactatgc tcctcccata caccggctaa
      901 gtgtaccagt gtttcggcgg ccttctccat atcctgtacc gacacccatt ccaaacggga
      961 gtgaaaagcg tgttcgcctg caaaaatatt ggggcagggc agtcccataa aggataggcg
     1021 agagccgtct gtgccgccgc gaatgctcga taaatgcggc gtgatgcctg ctcttttgat
     1081 ggcttccaca gcgtgggcgg ccacttgcgg gtagtctttg agcacctctt tcatgttgcg
     1141 gtactgctca atgatctcca tttttccttt ggcggcgggg tattcggcca agacggattc
     1201 catgatggcg cggagttcgt cttcgtgttt tttgaggcct tccaacgtaa aatcgcgcac
     1261 gatgaaacgg atggttgccg actccaaaga gccggaaatg aggcccggat gaacaaaccc
     1321 ctcgcggccc tcggtggtct ccggcgagag gcggtctttg ggcaggcgct caataatggc
     1381 ggcagctatc ttgatggcgc tcaccatttt acctttggca aaccccggat gcgccgatac
     1441 gccctcaatg gtgatgacgg ccgcatcggc cgagaaggtt tcatcttcga tagagccgag
     1501 gcgctcgccg tccaccgtgt aggcaaactg tgctccgagc tttttgatgt ttaccttgtc
     1561 cacgccgcgt ccgatctcct catcgggcgt gaataggatt ttaatgacgc cgtgtttcca
     1621 ttccgggtgt gtcatcagat agtgtgctgc gtccatgatg gcggccacgc cggctttgtt
     1681 gtctgcgcca agcaaggtag tgccggaggc ggtgacgatg tcgttgccga tctgttcgag
     1741 cagatcggga tgctctttgg cgcggatgac ttgagtgggg tcatctggca gcaccaaatc
     1801 ctggccctga taattgcgat gtacgatggg ctttacgcct gcgccggagg catctggcga
     1861 ggtgtccatg tgagagcagt agcaaataac gggcacgttt ttggtcgtat tggcagggat
     1921 ggtggcataa acatagccgt acttatccaa tttggcatcg gaaataccga tttcatgcaa
     1981 ctctttgacg agcagtttgc ccagattttt ttgcttttcc gtggagggga tggtttttga
     2041 ttcaggatcg gattgagtat ctatgacgac gtagcgcagg aagcgatcca gcacctgatg
     2101 ttgaaaggaa agtgacatga ttttgctgtg ttttgtgggg ggcaaaggta cggctttggt
     2161 tgggaaaagt gcataaaaaa ccccgtccgt tcttgcttcg gaaagacgag agtcggacgg
     2221 ggtgggttgg aatgataaac tttacccttc tttgacaaat agtgtgggaa cagatttagc
     2281 ggaaggcagg acgactgtaa ggaggcatga ttttcgctaa atctgttcct ttaagcacac
     2341 tatttgtcaa agaatcaact ttactttgtt tccaattctt ttgcaatgcc ggcgtgaact
     2401 gcttcaaatt gccgtttcat attttcataa tacgccccca ttgccgatgc ttgctccggg
     2461 tgcgtaaaga gatgatccag aagagctatc ttgttgatat ccagcccgga tttgtctttc
     2521 agtatggtgc gcgcatcgtc cagcatttcc ctctgagagg tatattgttt ggatgaaacg
     2581 ccggcaaaca gcacctcttt gtgcgtgggt gaaaccgagt agtcccgttc ctgaacggct
     2641 ccgaccatac tgccgccggt atcgtacaag cgcggtttga aattcggatt cacctcggca
     2701 atagccaatt gcatggcatt catttgagtc tgcatctcgt cgaggacatg agcaaagtca
     2761 ttctgattga gacctacggc ggtgttgatg tagttgatgt atctgtcggg atgggcatcg
     2821 ctccaggcca cgcccacgat gcggcggtag tcccgcacct tcatgttttt gggcgataca
     2881 gcaatggcca taccatcgtt ctcgcccgaa ggcaggatgt agtcgcctgt ttttaccggg
     2941 ccttttactt tcacaggtac ctggcccatg aaggcaattt tctcgtggtc tttctcgcgg
     3001 ttcttgtcgg taggcatatt ccccagcaca atgggcgaag tggagaccac catgaatcgc
     3061 tccgggttgg cgaattgttt cgacaccttt ccgcctatta ctcctactac gtctcccgga
     3121 tgcataatct cttccgtatt tgcccgctcc agccactctg cgtagtcggc agaaccagac
     3181 tgataagtga cgcccacatt ggccgaggtt gatgtcggca tagctgccat aaatgaccat
     3241 attggtgatt tcaacggctg ctcctatggc tgtactgagt acatcttccg ggtcgagtgg
     3301 gctggcaaag cttgccgcga actgtatcaa cgagccaagc gtagaaattc ctccctgaag
     3361 tatatccatc gaatagtcgg aaaatatttg tgacttgaag ttggtggcag cttcagggtc
     3421 tccaacaatg agaggaaaaa gtccggtagt ggtatcgcat acaatgtttt ggatgacatg
     3481 ccacaagatg ctggcaggtg tgccgttaag gtctctgcca ttgtagatat tcaaaaatga
     3541 cgcagaagga ctgctcaatg agctgttaat taaggcatta aagtctcccg gattgaaagc
     3601 ccctgtgttg aaaatcagat ttccatccgt aacaaagggg ttggaggaaa acgacaatgc
     3661 actgaagtca attccagtga tggcgctgcc ggcgaacaga ttgagcgtgg gcaattgtaa
     3721 aatgctggcc gggttcatgt tgagcgtaga gggcggggtg gtaatgatag acgatacaat
     3781 actcaacagt ccggtggggt cgagatctgc cttgctcatg ccttccactc tgccggtcat
     3841 ggtagctgag gagttccaga aagatatata attgttgccc ctgcccgatg ctaatgggct
     3901 aaagttagac ggatcaacct gaatcgccag tccttgacca ctgcccctga tgagcaaggg
     3961 gtagctgtct tgcgagcttt gacttccctg aatgtacgcg tcaacaatga ctctgttatt
     4021 cagccaggaa gggccttcta cttgtagata attgttgatt tgcacattgc tgtccacttg
     4081 gaggtcattg ccgatgtgta catcattccc gatgctgagg ttgatcccga cgtcggcatt
     4141 gccgcccaca aacaggtcat tgtccacaat cacggaatcg ccgctgaact gcacattgtc
     4201 ctgaaaatta gttcgcgcaa caatcagtaa ctcaccgttt ttggtgatgc gcatgcgctc
     4261 tacattgttg gttttgaaaa tgaaatccac gggtacggtg gtgcctatga aatggcaggt
     4321 gtcgttgaca ttgtagttgc caaggttggt ccaataagga ataccggtgg ggctgcaacg
     4381 tcggtttttt tccaattccg tcatcggcgc cgaccatgtt tcggcagtac ccgcatgaaa
     4441 ggcgtagggc acggccatga gttgtgtggc ggccagcgtt acaaaatccc cttttccggt
     4501 ttcatctaaa gccaaaacca tccaaatagg gtgttcgctc cagggtactt gccggaaatc
     4561 cccgcgctcg gttcggccct gcccaatgac gagttgaaac atgcccaact cattggtttt
     4621 caacaggtgt acttcttcat aagcggcttt cccgccggca tcgcccgctc gcaatgacac
     4681 gcgcaggcca atgttttgca gggctaaggg cgcccctttc gagtcgcgtg ctacggcctg
     4741 ataattgatg ccctgcggta tgctttgggc gaaagcgaaa aggctggtca aaagaaatgc
     4801 cgggatgatg aagaagtgtt ttttcatcgt tttgaatggt ttatgtgaat cgtgagaacg
     4861 gaaggaggct gcattctgtc gcctgcctcc ttccgcattg gcaagtcgaa tggtatcaat
     4921 ttcttcttgc ctccattttg aggatattat ctctggtagc cgcgtagtta tcctgcacat
     4981 tgcgaatgat ttcctgccgg aatacctgat cgctgaaaag ttttttcagt cnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnngct gcggttgtgc tgatagcggc gcgattggtt tgcccgattc caaagcggcc
     5221 aatcgtcggt tcagggcagc gaaatcattt ttcagatcgg ttacttcctg attcaatttt
     5281 tcgatttcgt tttgctgctc ttccaccaat tttgctatgt cattgttgtt caggccaata
     5341 gccatgttaa tgtaggccaa tttgcttcct gccggagttg ccgaccaagc cacgcctact
     5401 atttggcggt attggcccgg actgatgcgt tgcggagata cggccatgcc ggtaccgtcg
     5461 ttgaacccgg aaggcaggat gaagtcgcct atattgacct ggccgcgcac tttcacagga
     5521 atttgcccca taaaagccac tttttcaaac agcgcttcgg gataggtaac cagtatcttc
     5581 ttgtttcccn nnnnnnnnnn nnnnnnnnaa atgactaaat actgcgatac tccgttatcg
     5641 gtacgtttac tgatgagacc gccctttacc cctacaatgt ctccgggagt catggtttcg
     5701 gcggcatggc tgcgtttcag ccactctgcg tagtcggcag aaccagactg ataagtgacg
     5761 cccacattgg caaaagcaaa tgcctcataa gcagcgaggt tggcgatggc taatatttct
     5821 tcggctatgg caatggctac caccgacggc tccggcggac aggcaacggc gcctacgccg
     5881 gcacaggcat tgggcaaggc ggaaagtccg atattgacgc cggcggcaac gacttcggca
     5941 gtgtaaatag atgtttcaaa aatgaactcc ggagaggatg ctacttccgc tgaggtttgc
     6001 ccttcaatac ggccgcgggc agtattattg ttgtcaaaaa aagtaatgaa gttgttgctg
     6061 ccgttggggg ttccctgatt tactttcacc gcaatcccct gagcgctgcc ttccactcgc
     6121 agcggatagg cattgtaaga cgcatcgccg ccacctacat tggctctgat ggtaacctgc
     6181 ccgttcaaat cggttacgtt gtttactgtg agcttgccgc tggaagtgag ggttccgcca
     6241 acggcagtat tgcgggttac tgccaggtct cggccaatgc tgccgtctcg cccaatgcta
     6301 aggtcgttgc cgatttcggc attgttggtg acaaaaatat tggtaaaccg gccatcgccg
     6361 cctacctcca gattgcctga gatgcttccg tttccgccca cgccgaggtc ttgtccgatg
     6421 ccgccattgc ctcccacatc caagttttgg tctatggcgg cgtctccatg cacatccaaa
     6481 tcttgcccca cgtccacatt gcctcctaca aacaggtcgt tgtctatgat gacggagtcg
     6541 ccgctgaact gcacattatc cacaaacgtt accctggcaa ggatgatcaa ttctccctct
     6601 ttggtgatgc gcatgcgctc cacattgttg gtcttgaaaa tgaaatcaac aggtacggtg
     6661 gtgcctatga aatggcaggt gtcgttgacg ttataattac caaggttgct ccagaaagga
     6721 ataccggtgg aactacagcg ccgg
//
LOCUS       DAORLF010000268         6744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6114_length_6744_cov_2.047092, whole genome shotgun sequence.
ACCESSION   DAORLF010000268 DAORLF010000000
VERSION     DAORLF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_6744_cov_2.047092"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    262..287
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<288..748)
                     /locus_tag="PK198_18220"
     CDS             complement(<288..748)
                     /locus_tag="PK198_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRF40736.1"
                     /translation="MKIKVCGLRNPDNILAIAALPVDFIGLILYAGSSRNAASEQMIE
                     WLEQNESALAGVKIAGVFVNAEIEDVLNAVHDYRLDFVQLHGDESPDYCRELDSFRSL
                     SSMRTAKLIKAFRVDEQFDFSSVAAYASWCSYALFDTRSAEYGGSGRHFDW"
     gene            complement(767..1582)
                     /gene="trpC"
                     /locus_tag="PK198_18225"
     CDS             complement(767..1582)
                     /gene="trpC"
                     /locus_tag="PK198_18225"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRF40737.1"
                     /translation="MNILDNIVAHKKTEVALHRQKFPIAVLESSIYFERPVYSLSEAV
                     RRAPGPGIIAEFKRRSPSAGEIRAGASVQEITAGYMEAGAAALSVLTDKQFFGGRNGD
                     LSEARYVNSGPILRKDFVIDEYQLIEAKAIGADAILLIAACLSVPGCRELARFARSLG
                     LEVLLELHDAAELSHVNEYVNAVGVNNRNLKTFEVSLATSFDIVERLPSELLKVTESG
                     INDPAAAADLLHAGYKGFLIGEHFMRQPDPGAACRDFIQRVNDRNQLLNGAIA"
     gene            1743..2186
                     /locus_tag="PK198_18230"
     CDS             1743..2186
                     /locus_tag="PK198_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRF40738.1"
                     /translation="MDNDRNSKQDSVYSLKMKAGKRRTYFFDIRKTKGDDFYLTLTES
                     AKRMNGDGYERHKIFLYKEDFNRFLSNLQEAIDHVKTELLPDYDYDEFNHPDDYEGEY
                     QGPGRREEQAPRRPRFESEAEYNPRHAEEKPTSPELPADEDDMSW"
     gene            complement(2189..2767)
                     /locus_tag="PK198_18235"
     CDS             complement(2189..2767)
                     /locus_tag="PK198_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40739.1"
                     /translation="MRTLILAIPFCLSCMLATAQSPNAARFRWELGSNIGLMPTFAKD
                     RPDMLMLPVSAYVDYRLAPNFSLSATGGHSRSALVRNSSITGEPLYYENSYSYVGLRL
                     AAHTSPYRFDRWDIYGGLSGMLVFSDISVGPLNKSASVLASNQRHNKFVLTGFLGARY
                     ALTPRWGIQAELGFGASLLNLGISYRFAKTRK"
     gene            complement(3115..>4389)
                     /locus_tag="PK198_18240"
     CDS             complement(3115..>4389)
                     /locus_tag="PK198_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40740.1"
                     /translation="AVYNSMNSEARNAIAVRAFDFELRQALKNACDDGLYDVRNVFDA
                     GLVAAGASGFNSVTFINNHDYRTPGEHILNRMMLAYAYILTNNKVGLPCVFYPDYYGV
                     DIYGPSNPIASLQTQIDQLMQVHKNYIAGATFVDYLNREGTPYNSAYLQSGPYDLLLY
                     QIQYGPVPGKDVIVAINFENQPLRVNHTINTARAPLGTQFNLAAGTANYTTPVVENSS
                     NGIPNSIYLDLPAYSYAVFVQGALLPVTLTTFEATGKQKSVQLTWAADTEDQFAGYEA
                     QRSLDGQSYQKIAWVDAKGTAGGPARYELYDNEPAFNQPMYYRLRMVDLDGTAEFSPI
                     RSVTLQQKAEAVLFPNPSKGQFFIRFEEAVQPGTQLEIFDALGRSMARRILEEGALLE
                     ELSLDGAENGVYRVRVTKAGQVVFNGVLHHIR"
     assembly_gap    4390..4404
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4405..>4775)
                     /locus_tag="PK198_18245"
     CDS             complement(<4405..>4775)
                     /locus_tag="PK198_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40741.1"
                     /translation="IIQDLAVQTRTNFSGLPSGKGGMNYLNFKPNGSQFTCMTGDEEY
                     PYFFFDVEQTHPTTGTAYRDWNKFLWDSVGIRGYRMDAVKHFPAWFVGALLNEMHAAG
                     MNPPMVVGEHFTSFAPTLKGWV"
     assembly_gap    4776..4859
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4860..5692)
                     /locus_tag="PK198_18250"
     CDS             complement(<4860..5692)
                     /locus_tag="PK198_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40742.1"
                     /translation="MYRYLLILMLGALPVLASAQDFMMQGWYWDYPKPGCNSYTGPSL
                     AAEMAARAAAQKNAGFTMMWLPPMTKASFGNCSNGYDPKDIYDYGQYSGATGLGAKAE
                     IQNWLSALSSNNILPVADVVYNHRDGGDWEDNPAVRNYIGIPPSGPWPGCGSATPYPV
                     NGKLRYRLPLGGSSGNGAGDYYFKFSSASGNAGFNGRPYKLYFRTQNTTFVNDPINES
                     EPNGGSDCGQPNKQIFLGKDIFAVQEVGGGCNTDAFYLNLGAGDFNSAGDNIEIYIEE
                     VG"
     gene            5955..6734
                     /locus_tag="PK198_18255"
     CDS             5955..6734
                     /locus_tag="PK198_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF40743.1"
                     /translation="MKYFPVLLFCCLISFFAGAQNELNTRSPYGIQDASKFEMLMPDL
                     SGFGGSARDMLRQQSVKPYLMPVRKLGERGSEATYMAAACLEYYINRDQNFKLNLSPD
                     FIGLSLGKSASLRDALVFLIERGTVNAAVVPYDAPSVPEVAFSVPNFRISNFLHVFRD
                     LSPARQRVFETKKALVRGHPVLVELKTDEAFRQFSGEAFLSPGNGSQIHTVLVVGFDE
                     AAGAFEVQTCRGYDWGQNGYALIRFDDFDRAALNGYVLVRD"
BASE COUNT         1622 a         1776 c         1689 g         1532 t
ORIGIN      
        1 ggctgcgcaa ttcctctata ttggcaaaca acatttcggg aacgaaagcg ccgccaaatt
       61 cgccgtaata tcctttctcg tcaatggctg tcatctgtga agcgtttgaa tgaaacgatt
      121 gattttttcc acatcctttt cagcgggcgc cgtctcgaag cggctgttga tgtccacccc
      181 caacatcaag ggatgacgga attggagcaa ggcctccgca tcttcctctg caatgccgcc
      241 gctgagcaaa aaaggcagcg gnnnnnnnnn nnnnnnnnnn nnnnnnntcc cagtcgaaat
      301 gcctgccgga gccgccatac tccgccgaac gggtatcgaa cagcgcgtaa ctgcaccagg
      361 atgcgtaggc tgcaacggaa gaaaaatcaa attgttcgtc cacccgaaaa gccttgatga
      421 gcttggcggt gcgcatggaa ctgagcgagc ggaaactgtc caactcgcgg cagtaatcgg
      481 ggctttcatc gccgtgcagt tgtacaaaat ccaaacgata atcgtgtacg gcattgagta
      541 cgtcctcaat ttcggcattg acaaaaacgc ccgcaatttt gacgccggcc aaagcagatt
      601 cattctgttc caaccattcg atcatctgtt cggaggccgc atttctcgaa gaaccggcat
      661 acaaaatgag gccgatgaaa tcaaccggca gcgctgcaat ggcgaggatg ttatcggggt
      721 ttctcaaccc gcatactttg attttcatag agttatggaa tgagattcag gcaatggcgc
      781 cattgagtaa ttgattgcgg tcgttgacac gttgaatgaa gtcgcggcaa gctgctcccg
      841 gatcgggttg ccgcataaag tgctcgccga tgaggaagcc tttgtagccg gcgtgcaaca
      901 gatcggcagc ggcagcgggg tcgttgatac cgctttcggt cactttgagc agctcggatg
      961 gaaggcgctc gacaatatca aaagaggtgg ccagactgac ctcaaaggtc ttgagattgc
     1021 gattattgac ccccaccgcg ttgacgtatt cgttgacatg gctcaactct gctgcatcgt
     1081 gcagttccaa caacacctct aagccgagcg aacgggcaaa acgggccagt tcccggcagc
     1141 ccggcacgct gaggcaggcc gcgatgagca aaatggcatc cgccccgatg gctttggcct
     1201 ctatgagttg gtattcgtct atcacaaaat ctttgcgcaa aataggcccg gaattgacgt
     1261 agcgggcttc agaaagatcg ccgttgcggc ctccgaagaa ctgtttatcg gtgagtaccg
     1321 acagtgccgc agctcccgcc tccatatagc cggcagtgat ttcctgcaca gatgcgccgg
     1381 cgcggatttc gcccgcagag ggagagcgcc gcttgaattc ggcaatgata ccggggccgg
     1441 gcgcccggcg aaccgcctcg cttaaagaat acaccggtcg ctcaaagtag atgctgctct
     1501 ctaaaacagc aatgggaaac ttctgccggt gcagcgccac ctcggttttt ttgtgcgcca
     1561 cgatgttgtc taaaatattc atacccaaat aaccgcgccg ggaaaggtga gcatcggcgt
     1621 cggaacagcc gcaaagttgc ttttatccta cgattttttt ttcagcaatt ggatattttc
     1681 atcaaatact tacttttgtc atggattgtt actttttcgt gtaaacataa atttcaattc
     1741 gcgtggataa cgacagaaat tccaaacaag acagcgtcta ttccctgaaa atgaaggcgg
     1801 gaaagagaag gacttacttc ttcgacatca ggaaaaccaa aggagacgac ttctacctca
     1861 cacttacgga aagtgccaaa aggatgaacg gcgacggcta tgaacggcac aaaatctttt
     1921 tgtacaaaga agatttcaac cgctttcttt ccaatttgca agaggccata gatcatgtaa
     1981 aaacggagtt gctgcccgat tacgactacg acgaatttaa tcaccccgat gactacgagg
     2041 gcgaatacca aggcccgggc cgccgcgagg aacaggcgcc gcgccgcccc agattcgagt
     2101 cggaggcaga atacaatccc cgacatgccg aagaaaaacc gacatctccg gaattgccgg
     2161 cagacgagga cgatatgagc tggtaaactt attttctcgt tttcgcaaag cggtaggata
     2221 tccccagatt gagcagcgag gccccgaatc cgagttctgc ctgaatgccc catctcggag
     2281 taagtgcgta ccgcgcgccc aaaaatccgg taaggacaaa tttgttgtgc ctctgattgc
     2341 ttgccaaaac agaggccgac ttgttcagcg gcccaacaga tatatcgctg aaaaccagca
     2401 ttccggacaa gccgccataa atatcccagc gatcaaaacg gtatggcgag gtatgcgccg
     2461 ccagtctcag cccgacataa gaatagctgt tttcataata caaaggctcg ccggtaatgc
     2521 tgctgttgcg taccaaggcc gagcgggaat gcccgcccgt tgcgctcagg ctgaaattgg
     2581 gggctaagcg ataatctaca taggcactca ccggcagcat cagcatgtcg gggcggtctt
     2641 tggcaaaggt gggcatcagc ccaatattgg agccgagttc ccaacggaaa cgcgcggcgt
     2701 tcggagattg agccgttgca agcatacaac tcagacagaa aggaatagct aaaatgaggg
     2761 ttctcatggc agtatggttt tttaaaaggt gaaaaagaca atgaggaatc agtacttcca
     2821 atggtaagat tgaaagaaaa aggaataaga agcagtacca agcggcgtcg gaatcagcag
     2881 ccgggttcag tctgaaaaac tacaaaagca aaagcagcag cagaccggga tcaaaagggc
     2941 gaagcaatcg ttttcgtttc ataagcataa ggtttataaa gggttgtaaa aatggtgtgt
     3001 gtgtgattta cattcctata gacgcgcaaa acgcggcctt cgttgatcga aaaccgcgtt
     3061 ttaacttatt attttattgt aaagttccgg cttggtcttc ctgccgacaa gcccctacct
     3121 gatgtggtgc aaaaccccgt tgaaaaccac ctgcccggct ttggtaacgc gtactctata
     3181 aacgccgttc tccgctccgt ccaaactgag ttcctccagc agggcgcctt cttccagtat
     3241 tcttcgggcc atgctgcgcc ccagcgcatc aaatatttcc aattgcgtgc cgggctgtac
     3301 cgcctcctca aatctgatga aaaactgccc tttggaggga ttgggaaaca acaccgcctc
     3361 cgctttttgc tgcaatgtga cgcttcgtat gggcgagaac tctgccgtgc cgtctaagtc
     3421 caccatgcga aggcgatagt acatgggctg attgaacgca ggctcgttgt catacagttc
     3481 gtaccgggcc gggccgccgg cagtgccttt ggcgtccacc caggctattt tctggtagct
     3541 ctgcccgtcc aaagagcgct gcgcttcgta gccggcaaac tggtcttcgg tgtctgcggc
     3601 ccaggtgagt tgaacggatt tttgtttgcc ggtggcctcg aaagtggtga gagtgacggg
     3661 gaggagcgcc ccctgtacaa acacagcata agagtaggcc ggtaggtcga gatagataga
     3721 attgggaatg ccgttggagc tgttttcaac tactggtgtg gtataattgg ctgtgccggc
     3781 cgccaggttg aactgcgtgc cgagcggcgc cctcgcagta ttgatggtgt gattcactct
     3841 aagcggctgg ttttcaaagt taatggccac aatcacatcc ttgccgggaa caggcccgta
     3901 ttgaatttgg tacaacaaca aatcataagg cccggattgc agataagcgc tgttgtatgg
     3961 cgtaccttca cggttgagat agtcaacgaa agtggctccg gctatatagt ttttatgcac
     4021 ctgcatcaat tgatctatct gtgtttgaag cgaagcaatg ggattggagg gaccgtaaat
     4081 atctacacca tagtaatcgg ggtaaaagac gcagggcagc cccactttgt tattggtaag
     4141 gatgtaggca taggccagca tcatgcggtt gaggatgtgc tcgcctggcg tccggtaatc
     4201 gtggttgttg atgaaagtga ccgaattgaa cccacttgct cccgcagcta caagacctgc
     4261 atcgaaaaca tttctgacgt cgtacagccc atcgtcacag gcatttttta gcgcctgccg
     4321 aagctcaaag tcgaacgccc gcactgcaat ggcatttcgc gcctccgaat tcatactgtt
     4381 gtacaccgcn nnnnnnnnnn nnnnacccag cctttcagcg tgggcgcaaa cgaggtaaaa
     4441 tgctcaccca ctaccatcgg cggattcata cctgctgcgt gcatttcgtt gagcagcgcg
     4501 ccaacaaacc atgccggaaa atgcttcacg gcatccatcc gatagccccg aataccgacg
     4561 ctgtcccaaa gaaacttatt ccagtcccgg taggcggtgc ctgtagttgg gtgagtttgc
     4621 tctacatcga aaaagaaata ggggtattcc tcgtcgccgg tcatgcaggt gaactgagac
     4681 ccattgggtt tgaaattcag atagttcatc ccccctttgc cgctgggcag gccggaaaaa
     4741 ttggtgcggg tttgtacggc caggtcttgg atgatnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4861 ctacttcttc gatatatatc tctatattat cgcctgccga gttgaaatcc cccgcgccaa
     4921 gattcaagta aaacgcatct gtattgcagc ccccgcctac ttcttgcaca gcgaagatat
     4981 ccttccccag aaatatttgc ttgttgggtt gtccacagtc gctgccgcca ttgggttccg
     5041 attcattaat aggatcattg acaaaagtag tattctgtgt gcggaaatat aatttatacg
     5101 gcctgccatt gaatccggca ttgcccgaag cagaactgaa tttgaagtaa taatcccccg
     5161 ctccattgcc gctgcttcca cccagcggca ggcgatatcg cagctttccg ttgacaggat
     5221 aaggagtagc gctgccacaa cccggccaag gccccgatgg cggaatgccg atgtaattcc
     5281 tcactgccgg gttgtcttcc caatcgccgc cgtcgcggtg attatacacc acatcggcta
     5341 cgggcaggat gttgttggac gacagtgcgg aaagccagtt ttgaatttcc gctttggcgc
     5401 ccaatccggt agcgccagaa tactgcccgt aatcgtaaat gtctttggga tcatagccat
     5461 tgctgcaatt gccgaaactc gccttggtca tcggcggaag ccacatcatg gtgaaacccg
     5521 cattcttttg agcggcagcc ctggcggcca tttcagcagc caatgacggg cctgtgtagc
     5581 tgttgcatcc cggcttggga taatcccaat accagccttg catcatgaaa tcctgcgccg
     5641 acgccaacac cggcagcgcc cccaacatca gaataagtaa atagcgatac atgattcgtt
     5701 tggttaatga gtgttcggat aaaaagtacg ctcgtttcgg cagcgtagtg aaggggggac
     5761 tccgccagga cacaaccccg tagtcacccc ttcactaagc gcataaaagt acgttttcaa
     5821 accgccgcac ggccttttcg ttcgtcattt cggcaaggat tttacaggat gcaaattttc
     5881 atcattccgc tcacattttc aagacgaaaa ccgcccgccg cttcgtttgg cacttgcacc
     5941 aatgctgatg agtcatgaaa tactttcccg ttctcctttt ttgctgtctg atttcctttt
     6001 ttgccggcgc tcaaaacgag ctgaacacgc gctctcccta cggcattcag gatgcctcca
     6061 aatttgaaat gctcatgccc gatctcagcg gttttggcgg ctcggcccgc gatatgctgc
     6121 gccaacagag cgtcaaaccc tacctgatgc cggtgaggaa attgggcgag cgcggctcgg
     6181 aggccaccta catggcggcg gcctgcctcg aatattacat caatcgcgac caaaacttca
     6241 agctcaatct cagcccggat ttcatcggcc tcagcctcgg caaatccgca agcctccgcg
     6301 atgccctcgt ttttctcatc gaacgcggca ccgtcaatgc agccgttgtt ccctacgacg
     6361 caccctccgt acccgaagtg gccttttcgg tacccaactt ccgcatttcc aatttcctgc
     6421 acgttttcag agacttgtct ccggcgcgcc aacgtgtttt tgaaaccaaa aaagccctcg
     6481 tgcgcggcca ccccgtactc gttgaactga aaaccgacga ggccttccgc caattttccg
     6541 gcgaagcctt cctctctccc ggcaacggca gccaaataca cacggtattg gtcgttggct
     6601 tcgacgaggc cgccggcgca tttgaggtac aaacctgccg gggctacgat tggggccaaa
     6661 acggctacgc gctcatccgt ttcgacgact tcgaccgcgc cgctctcaat ggctatgtgt
     6721 tggtaaggga ttgacaatca aagc
//
LOCUS       DAORLF010000269         6730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6137_length_6730_cov_1.994457, whole genome shotgun sequence.
ACCESSION   DAORLF010000269 DAORLF010000000
VERSION     DAORLF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_6730_cov_1.994457"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>385
                     /locus_tag="PK198_18260"
     CDS             172..>385
                     /locus_tag="PK198_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40744.1"
                     /translation="MARQDPLHQLIRGLEKGEIRHFRLLARMHYSKDKRSDYEELFDL
                     MAAQEQYDEAATARSLSNQLFARNLSV"
     assembly_gap    386..427
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <428..>1667
                     /locus_tag="PK198_18265"
     CDS             <428..>1667
                     /locus_tag="PK198_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40745.1"
                     /translation="LRHYYAEENLYFQVREMWMDAFLLMRKGLHKQALKVLEKAMKLC
                     SEKGLYAHFFDFSHMKGRHDLSYANLTSPDELNKLNDADNVQYIRLGNQITTNYVITI
                     TDAASRGGFLSDSEWEQMKEFYGPLLKKMEEDGQAKHDVLSYYLPMVKYYNFKKEYDK
                     SYEIQEEILRHLVYKDNTMVKAAPRRYFAFCVNFFNVCSFLLSQEETAGRPDKYSRME
                     ELIGQMKQVRPESKEEGLFFKSDILYSEVLYYALSEKYEGILNMKNEPVEILKEVGPL
                     MPLSKQWATCINIASAYMYVGRFSEALDWLNIIFQNQDNDTLPSHQIINRIYMMVCHF
                     ELGNAGFLEHLERNIVLFIRRQNPDFKKRVTPTFNAMRKVIYVQSQKNDFKQALLQIE
                     ELTRKEVSFISVHRWAVQKLT"
     assembly_gap    1668..1689
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1777..3174
                     /locus_tag="PK198_18270"
     CDS             1777..3174
                     /locus_tag="PK198_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRF40746.1"
                     /translation="MTNKYFDLIDQTFYFPQEGFDLEDGYLTFNGVPLIYLIRKYGTP
                     LKITYLPKIGLQIKKARNLFTRAMRSMGYSGDYYYCYCTKSSHFSYVLNEVLRHDVHL
                     ETSSAFDIELIRRLHAKEKIEKDTIIICNGFKPENYMGQITNLINDGFSNVIPVIDNM
                     HEVDFYEKHVEGICNVGIRVATEEEPNFEFYTSRLGIRHAEVMKFYKERLADNPKFKL
                     KILHFFVDTGIRDTLYYWGELKKAIRLYCELKHICKDLHGINIGGGMPIRNSLGFEFD
                     YKYMIKEIVRLISEECEEAGVPEPDIFTEFGKYTVGESGAVIFSVLAQKQQNDAEVWY
                     MIDNSLMTTLPDAWGIGERFVLLPVNKWDSDYRRVNIGGLSCDNSDYYNSEVHQSQVY
                     LPSFQPNEEEPLFLGFFHTGAYQDSISGYGGIKHCLIPSPKHILLYRDSQGNLVDELY
                     QPEQSADEMLKILGY"
     gene            complement(3313..>5949)
                     /locus_tag="PK198_18275"
     CDS             complement(3313..>5949)
                     /locus_tag="PK198_18275"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40747.1"
                     /translation="KTMLYSIPCSGIITGTEFDNFFPFSRKYILALKGRQLGVLDWCG
                     AKTLEVAYDEIQPVDGQVFRVQREGKWGLHAAQGIELTPLRYDYIAPAVGAVCLIRLN
                     GRFGLLNTKGKEMVAPDYVRIELSGNEAKAYKKGPDGNETLALFEWNDSGEISDNNSF
                     NRHFRIRIGGLPDSSERRAVFNDNNFLLDDFEWFYAASKARWGLRRRSDGMVQIEPKF
                     HSIQVYPEFGFTLAALQQNTRGEFERTSFRFESVFGLVNNQLGRLVTEPVFLDVRMSD
                     FQQGLPAARCVLSDGRHGLVDRMGRVLRKDVAFIGEFQEGLARFALGGELSGSLKPDS
                     NHLNKLSDYLAQLKSPVSMTDYTEYDQLFQKNALLICRDCQWGYIDTLGRIVVQAQYH
                     FARDFVNGVGIVSCDGSKWGMVNRKAEAILPCRYDGVQFLENTDNQIIRVYIQQPKYG
                     LIDTLGRLTVSAIYDEIGSFTEGRLAVKRNGLWGYVDRDGLEVIPCRFRDAQSFSGGL
                     AAVKLGRFWGYIDKLGDIGIGFKWARAGSFHEGLAWVYDNNAFGYIDSSGNLAIPAKF
                     ERSFDFQNGVARVTADGKTGLIDNTGRYALRPKFADMQPFNQHGLALVRFGGKWPKYG
                     MADRSGRLITQQAFQEIRPFREGLAAVRYKNNYGFIDTTGKIAVTASYSKVADFVNGR
                     AAVYSLGRCGYIDAAGRAVTPIEYSKCLDFEQDKAIAYKGVREAHLIDMEGIANKVAE
                     GADRLLRFSEGRGLMRTESYSFYYIAEQTGAYTDAYQTATPFRHGVAVVENNGKWGII
                     DQRGIAIIPPKYDKIESFENGYAKVRILGFSGLTNLHGELIVQPDYEYITYAGEGLFR
                     VEQGDKIGYFDQQGKWVWDLAQ"
     assembly_gap    5950..5970
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5971..>6388)
                     /locus_tag="PK198_18280"
     CDS             complement(<5971..>6388)
                     /locus_tag="PK198_18280"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40748.1"
                     /translation="VMDAGEWMVVDLQGRTVLEKGYERIRVLKNGFLAFRKQNKWGLV
                     NHRGYTVAEPLYDDIRQETEQWFLASKGGKFGLISAQGKEVIPTHAEVIQLFNDSLFF
                     YKINQQWGAADAAGRTVIQPRFEGFQKLSEHFIKLFG"
     assembly_gap    6389..6409
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6410..6657)
                     /locus_tag="PK198_18285"
     CDS             complement(<6410..6657)
                     /locus_tag="PK198_18285"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF40749.1"
                     /translation="MKKAIILWCLVLTGSLSAQQAFPVKVNKKWGLIDAAGQVILPPV
                     YEAIGEFRHFGHATMQRNGKVGLLDEQGKEVIRPVYEDL"
BASE COUNT         1701 a         1796 c         1555 g         1572 t
ORIGIN      
        1 aatgagtatc atagctgaag tttttgatga ctgatgatgt tgatacaaaa ttacgggcta
       61 tgacaaaatc cgtcaaaccg cgttttttaa aaaataagaa tcccataagc cggaacaagt
      121 agaaatagtt cctatttttg tagatatttc atcaaataac attacaatca catggctcgg
      181 caagacccgc ttcatcagtt gatacgaggt ttggaaaaag gagaaatccg gcattttcgg
      241 ctattggccc gtatgcacta ttccaaagat aaaagatcag attacgaaga gctgttcgac
      301 ctcatggccg cacaagagca gtatgatgaa gcagcaactg cgcgatctct gagcaaccag
      361 ctatttgccc gcaatttgtc ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnatt gaggcactat tacgcggaag aaaacttata ttttcaagtg cgggagatgt
      481 ggatggacgc ttttttattg atgcgtaaag ggcttcacaa acaggccctt aaagtgcttg
      541 aaaaggctat gaaattgtgt tcggaaaaag ggctgtacgc acattttttt gatttttcgc
      601 acatgaaagg caggcatgac ttgtcgtacg ccaacctcac cagccccgat gaattgaata
      661 agttgaatga cgccgacaac gtgcagtata ttcgtctggg caatcagatc acaaccaact
      721 atgtcatcac catcaccgat gccgcctcaa ggggcggttt tttgtctgat tcggaatggg
      781 agcaaatgaa ggagttctac ggccctttat tgaagaagat ggaagaagac ggacaagcca
      841 aacacgacgt tttgtcgtat tacctgccga tggtcaaata ctacaacttc aagaaagaat
      901 atgataaatc ttacgaaatt caggaagaaa tactccgtca tttggtctat aaagacaata
      961 cgatggtgaa agccgcgcca agaagatatt tcgctttttg cgtcaatttt ttcaacgtat
     1021 gtagtttcct tctatctcaa gaggaaacag ccggcaggcc ggataagtat tcccgtatgg
     1081 aagaactgat cggacagatg aaacaggtac ggcccgaaag caaggaggaa ggactgtttt
     1141 ttaagtccga tattttgtac tcggaagtat tgtattacgc tctttctgaa aaatacgagg
     1201 gtattctcaa tatgaaaaat gagcctgtgg aaatactcaa ggaagtaggc cccctcatgc
     1261 ctttgagcaa acaatgggcc acttgtatca atatagcctc agcttatatg tacgttggtc
     1321 gtttctctga agccttggac tggctcaaca tcatctttca aaatcaggac aacgacacgc
     1381 tcccctctca ccagattatc aaccgcatct acatgatggt atgccacttc gagttgggca
     1441 atgccggctt tttggagcac ttggagcgaa atattgtgct gtttatcagg cgccaaaacc
     1501 cggatttcaa aaaaagggtg accccgactt tcaacgccat gagaaaggtt atatatgtgc
     1561 aatcgcaaaa aaacgatttc aaacaagccc ttttacaaat agaggagctt acccgaaagg
     1621 aagtgtcctt catctccgtt caccgctggg cagttcaaaa actgactnnn nnnnnnnnnn
     1681 nnnnnnnnnc acactttttc cctcgcgctt tgcgcatttg tgctactttt gccgccgcat
     1741 cattcttggt ttaacacaac tcaatctttc agggcggtga ccaacaaata tttcgatctc
     1801 attgatcaga ccttctactt cccgcaggaa ggtttcgacc tcgaagacgg gtacctcact
     1861 ttcaacggcg ttccgctgat ttacctcatc cgaaaatacg gtacgcccct caaaatcact
     1921 tatttaccca aaatcgggct gcaaatcaaa aaggcgcgca acctcttcac ccgcgccatg
     1981 cgctcgatgg gctacagcgg cgattactac tattgctact gcaccaaaag cagccatttc
     2041 agctatgtac tcaatgaggt actgcgccac gacgtgcatc tggaaacctc ctctgctttc
     2101 gacatcgaac tcatccgcag gctgcacgcc aaagaaaaaa tcgaaaaaga taccatcatc
     2161 atctgcaatg gtttcaagcc cgaaaactac atgggccaga tcactaatct catcaacgac
     2221 gggttttcca atgtcattcc ggtcattgac aatatgcacg aggtggattt ctacgaaaaa
     2281 catgtagagg gcatctgcaa tgtaggcatt cgggtagcaa cggaggaaga acccaatttt
     2341 gagttttata cctcccgtct ggggattcgc cacgccgagg tgatgaagtt ttacaaagaa
     2401 cgcttagccg acaatccgaa gttcaaactt aaaatcctgc acttcttcgt ggacaccggc
     2461 atccgagata cgctctacta ttggggcgaa ctcaaaaaag ccatccgcct gtactgcgaa
     2521 ctgaagcaca tctgcaaaga cctgcacggc atcaacatcg gcggcggtat gcccattcgc
     2581 aactcgctgg gcttcgagtt cgattacaaa tacatgatta aagaaatcgt gcgcctcatc
     2641 tctgaagagt gcgaagaagc cggcgtgccc gaacccgata ttttcaccga attcggcaaa
     2701 tacaccgtag gcgaaagcgg cgccgtcatt ttctccgtgc tggctcagaa gcaacaaaac
     2761 gacgccgagg tctggtacat gattgacaac tcgctgatga ctaccctgcc cgacgcctgg
     2821 ggcatcggcg agcgcttcgt gctgctaccc gtcaacaaat gggacagcga ttaccggcgc
     2881 gtcaacatcg gcggactgag ctgcgacaac tccgattatt acaactctga ggtgcatcag
     2941 agccaggttt atctgccttc tttccaaccc aacgaagagg agccgctttt cctcggcttt
     3001 ttccacaccg gcgcctatca ggattccatc agcggctacg gcggcatcaa gcattgtctc
     3061 atcccgtcgc ccaaacacat cttgctctac cgcgacagcc agggcaatct ggtggacgag
     3121 ctgtatcagc ccgaacaaag cgccgatgaa atgctgaaga ttttggggta ttgaggggtt
     3181 cgggtttcgg ggtttcgggt ttggaaccgc ctaccgccta ctcgatttcc tacgtcattg
     3241 ggacttcgct tcgctcggct gcataccgcc caccgaacac tctacgctta gcaccgccca
     3301 ccgaaaaacc cttcactgcg ccaaatccca cacccacttg ccttgttggt cgaagtagcc
     3361 gattttatcg ccctgctcta cgcggaacaa accctcgccg gcataggtaa tatactcgta
     3421 atcaggctgt acgatgagtt cgccatgcag attggtcagc ccgctgaaac ccaggatgcg
     3481 cactttggca taaccatttt caaaggattc aatcttgtcg tatttgggcg gaatgatggc
     3541 aatgccgcgc tggtcaataa tgccccattt gccgttgttt tccaccaccg ccacgccgtg
     3601 cctgaaggga gtagccgtct ggtaggcatc ggtataagcc ccggtttgtt cggcaatgta
     3661 atagaacgaa taactctccg tgcgcatcaa cccgcgccct tccgaaaacc gcagcaagcg
     3721 gtcggcgcct tcggccactt tgttggcaat cccctccata tcaatcagat gcgcttcccg
     3781 caccccttta taggcaatgg ctttatcttg ctcaaaatcc agacatttgg agtactctat
     3841 cggcgtcacg gcacgtcccg cagcgtcaat atagccgcaa cgccccagag aatataccgc
     3901 cgcccggcca ttgacaaaat cggccacttt ggaataggaa gccgtcaccg cgatcttgcc
     3961 ggtggtgtca ataaagccgt aattgttttt gtaccgcacg gctgccaggc cttcccggaa
     4021 agggcgtatt tcctgaaaag cctgttgggt aatcaatcgc ccgctgcggt cggccatgcc
     4081 gtatttgggc catttgccgc caaagcgcac caatgccagc ccatgttgat taaaaggctg
     4141 catgtctgca aatttggggc gcagcgcgta gcggccagtg ttgtctatca ggccggtttt
     4201 tccgtctgcc gtcacgcggg ccacgccgtt ctgaaagtcg aaactgcgtt caaatttagc
     4261 cggtatggcc aggttgccgg aggagtcaat atagccaaag gcattgttgt cgtaaaccca
     4321 ggccaaacct tcgtgaaaac tgccggctct ggcccatttg aacccaatgc cgatgtcgcc
     4381 caatttgtca atatagcccc aaaagcgacc taatttgacg gcagccagcc ctccgctgaa
     4441 cgactgcgca tctctgaaac ggcaaggaat cacctccagg ccgtcccgat ccacatagcc
     4501 ccaaagtccg ttgcgcttta cagcgaggcg cccttcggta aacgagccga tctcatcgta
     4561 aatagcgctc acggtgaggc gtcccaaggt gtcaatgaga ccgtacttgg gctgctgaat
     4621 atatactctg atgatctgat tatctgtatt ttccaaaaac tgcaccccat cgtagcggca
     4681 aggcagtatg gcctctgctt tgcgattgac catgccccat ttgctgccgt cgcaggatac
     4741 aatgccgacg ccgttgacaa aatcgcgggc aaaatgatac tgtgcctgca ccacgatgcg
     4801 ccccagagta tctatgtagc cccactggca atcgcggcaa atcagcagcg catttttctg
     4861 gaaaagttgg tcgtactcgg tataatcggt catggatacc ggtgacttca actgagcgag
     4921 gtaatcgctg agtttgttga ggtggttgga atctggcttg aggctgcccg acaattcgcc
     4981 gcccaaggca aagcgcgcca gcccttcctg aaactcgccg atgaaagcca cgtctttgcg
     5041 cagcacccgc cccatgcgat ctaccaatcc gtgcctgccg tcggaaagca cacaacgtgc
     5101 agcgggcaaa ccctgctgaa aatcgctcat gcgaacatcc aagaaaaccg gctccgtcac
     5161 caatctgccc aattggttgt tgaccaatcc gaaaacagac tcaaaccgga aagaagtgcg
     5221 ctcaaactcg cctcgggtgt tttgctgtaa agccgccagc gtgaagccaa attctggata
     5281 tacctgaata gaatggaact ttggttcgat ctgtaccatg ccgtccgagc gacgcctcaa
     5341 gccccagcga gctttcgagg ccgcatagaa ccactcaaaa tcgtcgagca aaaagttgtt
     5401 gtcgttgaaa acagcgcggc gttcggaaga atccggcaga ccgccgatgc ggattctgaa
     5461 atggcggttg aaactgttat tatcggaaat ttcgccgctg tcgttccatt caaaaagcgc
     5521 aagtgtttca ttgccgtcag ggcctttttt atacgctttg gcttcgttgc cgctcaattc
     5581 gatgcgcaca taatcaggag cgaccatctc cttgcctttg gtattgagca gcccgaaacg
     5641 cccgttgagc ctgatcaaac aaaccgcgcc caccgccgga gcaatgtagt cgtatcggag
     5701 cggggtgagt tcgatgccct gagcggcgtg cagcccccat ttgccctcgc gttgcacccg
     5761 gaacacctga ccgtccaccg gctgaatctc atcataagcc acctccagcg ttttggcgcc
     5821 gcaccagtct aaaacgccca attgccggcc cttcaacgcg agaatgtact ttctggaaaa
     5881 ggggaaaaag ttgtcgaatt ccgtgccggt aatgatgccg gagcagggaa tggagtacag
     5941 catggttttn nnnnnnnnnn nnnnnnnnnn ccgaaaagtt tgatgaaatg ttccgaaagt
     6001 ttctggaagc cctcaaagcg aggttgtatc accgtgcgcc cggcggcatc tgcggcgccc
     6061 cactgttgat tgattttgta aaaaaacaga ctgtcgttga aaagctgaat cacctctgcg
     6121 tgggtgggaa taacctcctt gccctgtgcc gagatcagcc cgaatttgcc gcccttagag
     6181 gcaagaaacc attgctctgt ttcctgccgg atgtcgtcgt acagcggttc tgccacggta
     6241 tatccccggt gattcacaag gccccattta ttttgttttc tgaaagccaa aaaaccattt
     6301 ttgagcacgc gaatccgctc gtagcctttt tctaaaaccg ttctgccctg caaatccacc
     6361 accatccact cgccggcatc catcaccgnn nnnnnnnnnn nnnnnnnnna ggtcttcata
     6421 taccgggcgt atcacttctt tgccctgctc atccaacagg cccaccttgc cgttgcgctg
     6481 catggtagca tgcccgaaat gtctgaactc cccaatagcc tcgtacaccg gcggcagtat
     6541 gacctgcccg gcggcgtcta tcaatcccca ttttttgttc accttgacgg gaaacgcctg
     6601 ctgcgccgac aaagagccgg tcagtaccag acaccacaga atgattgctt ttttcatagg
     6661 ccgaatgaaa cggttatgaa agtctccaat tgcctgaatg aaacgcccgg cggaaataca
     6721 aaagtatttg
//
LOCUS       DAORLF010000270         6707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6159_length_6707_cov_1.798857, whole genome shotgun sequence.
ACCESSION   DAORLF010000270 DAORLF010000000
VERSION     DAORLF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_6707_cov_1.798857"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PK198_18290"
     CDS             complement(<1..583)
                     /locus_tag="PK198_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRF40750.1"
                     /translation="MADLNAALASLHSGAEALRTLARTHGTAKVKHYMSALKALAGQA
                     LAEALQAIPDGEYEASEYLDDGSRLQVRVKHQAGHLDFDFGGSAPTHPNNLNANISIV
                     YSVVLYVLRLLVKKDIPLNEGLLSRVRIHLPEGTLLHPRFEDKGRACPAVVGGNTEVS
                     QRLTDTLLKALGMAACSQGTMNNFLFGNQQFGYY"
     assembly_gap    619..674
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<675..3288)
                     /locus_tag="PK198_18295"
     CDS             complement(<675..3288)
                     /locus_tag="PK198_18295"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRF40751.1"
                     /translation="MQHWHIWIDTGGTFTDGMAIAPDGAIIRIKVLSNSTLRGIVTER
                     TGAGVYQIEQQWNCSEDIFAGYRFRLLDGADGSALVQQILFSPTEPHKATLRLDRDFP
                     VSAAAGFEISAGEEAPVLAARIATRSPLHRSLPPLLMRLGTTKGTNALLERKGAPVTL
                     FITKGLADLPAIGTQQRPHLFQLDVPPPELLFERVIEVDERLAANGTPLLVPDEPHLA
                     DLAQQTPTGHSVAIALLHSYRNEAHELQLAAHLKNRGCRYISRSSELSQAIQLLPRAQ
                     TAIVNAYLEPVLDAYLSSIRGSIGDAALHIMTSAGGLVRAVHYRPKDSLLSGPAGGVV
                     GAAHIARQLGFERILTLDMGGTSTDCARYDGRYDYRFLTRVGQAEMLSPTLDIETVAA
                     GGGSICRFDGRKLCVGPESAGAWPGPACYGAGGPLSITDVNLLLGKMQPEAMGIPIFR
                     ERAEAALTAIRNQIREAGGAHYSEEELLRGFEQIADERMAAAIRRISVARGFDPRDYA
                     LLAFGGAGGLHACLVAELLGIATVLLPYDAGLLSAYGIGQARVERFAERQVLELLDEA
                     YPNLPGILWQLRAEAEAALNAEGFVSQEIEALHQLVRLRFRGQSSSVEIGWDGISDLQ
                     KAMERQYRHLFGHYPAGRAIEVESIRLVCGSRAGAGISHHFFSNEMPPAGTVLNGPAV
                     LPNRHSTAYIPAGWVLEFQAGGNAVIRHNEAAASNAGHGEAIELELFTNRFSAIAEEM
                     GAQLQRTAFSVNIKERLDFSCAILDADAELLVNAPHIPVHLGSLGVCTRLVKERLPLG
                     PGDVAMTNHPRYGGSHLPDVTLLSAVFSDEGERIGYVVNRAHHAEIGGKTPGSMPPDA
                     RTLEEEGVVIASMYL"
     gene            complement(3359..3937)
                     /locus_tag="PK198_18300"
     CDS             complement(3359..3937)
                     /locus_tag="PK198_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40752.1"
                     /translation="MNTSSHRFFFPLIAVATILSLCLLPFLTQAQAKPKWEEFRSYEG
                     RFRVLTPGAMRTKTDSIETAIGKLAYHTFFFEDAAQSTENVVYLLSYCDYPAGSLHHD
                     SIALVAEFFDASVQESAFSVAGDVAWSNDIKLGAYPGKSWRVDYKDKRAVIKTRAYVV
                     GNRYYSVQTVMKRERSLNAASDKFLESFRVFE"
     gene            complement(4104..4757)
                     /locus_tag="PK198_18305"
     CDS             complement(4104..4757)
                     /locus_tag="PK198_18305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF40753.1"
                     /translation="MYLVKTPQFIQKHFPAFTWKMPGKEPVLYLTFDDGPVPEVTPWV
                     LEQLEHYNAKATFFCVGDNVRKHSDVFHQVLDGGHAIGNHTYSHLNAWNTDNLDYFHD
                     VRRCAALTHSGLFRPPYGRLKPKQAQFLQRHYHIVMWDVLSGDFDTSISPEQCLNNVI
                     RNAGPGSIVVFHDSIKANERMSYALPRVLKHFSALGYRFETLAHLEMPALHTLRQTA"
     gene            5001..>5132
                     /locus_tag="PK198_18310"
     CDS             5001..>5132
                     /locus_tag="PK198_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF40754.1"
                     /translation="MNKIRVRFAPSPTGALHIGGIRTALYNYLLAKKFNGTFILRIED
                     "
     assembly_gap    5133..5237
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5238..>5932
                     /locus_tag="PK198_18315"
     CDS             <5238..>5932
                     /locus_tag="PK198_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF40755.1"
                     /translation="GDYGPYRQSERKAIYAEYAGQMVQNGYAYYAFDTPEELDAAREA
                     AKEAGNHTFKYDALERGSLRNSLTLPAETVSQLLEQGAPYVIRLLVQPGQDIVIQDLV
                     RGEVVFQSDELDDKILLKSDGMPTYHLANVVDDTLMEITHVIRGEEWLPSTAHHVLLY
                     RALGREAQMPSFAHLPLILKPDGKGKLSKRDGLRLGIPVFPIAWQGETPEDSFKGFRE
                     FGFLPQAMVNFMA"
     assembly_gap    5933..5948
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5949..6570
                     /locus_tag="PK198_18320"
     CDS             <5949..6570
                     /locus_tag="PK198_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRF40756.1"
                     /translation="GWNPGTEQEIFSMGELTDAFSIEKIGKSGARFDYDKARWFNQQY
                     LHATDDATLAGLVAPIVRANGYDPTEEQLQAFCRLMKERVTLLPEFWEKGAWFFAGVQ
                     EYDEANIKKRWSAERSLLFHQLLVELEALPEAAWQGEGEALKAAVETFMQANGLKPGD
                     VLPVLRIALVGNMQGPAVFELMAVFGRAETSARLAKAWERFSGVSV"
BASE COUNT         1463 a         1752 c         1892 g         1423 t
ORIGIN      
        1 cgtaatatcc aaattgctga ttgccaaaaa gaaagttgtt catcgtgccc tgactgcaag
       61 cggccatgcc cagcgctttg agcagcgtat ctgtcaggcg ttgacttacc tcggtattgc
      121 cgcctaccac cgccggacac gcccggccct tgtcctcaaa tcggggatgc agcagcgtgc
      181 cttccggcaa atgaatacgc acccggctca gcagcccttc attcagcgga atgtcttttt
      241 tgaccaacaa gcgcagcaca tacagcacca cgctgtaaac gatggagatg ttggcgttca
      301 ggttgttggg gtgcgtgggc gcactgccgc cgaaatcaaa atccaaatgc ccggcctgat
      361 gtttaacccg cacttgcagt cggctgccgt cgtccaagta ttcagaagcc tcgtattcgc
      421 cgtcgggtat ggcttgcagg gcctcggcca gcgcctgccc ggccaatgct ttgagggcgc
      481 tcatgtagtg ttttactttg gccgttccat gcgtgcgggc cagtgttcgc agggcttcag
      541 cgccgctgtg cagggatgct aaggccgcgt tcaggtcggc catattttca gcaagtgcgc
      601 gggtagggta ggggccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnccaaat acatcgacgc tatcaccacg ccttcttctt ccaatgttcg
      721 ggcatcaggg ggcatagaac ctggcgtttt acctccgatc tcggcatggt gtgcccgatt
      781 gaccacatag ccgatgcgct cgccttcatc tgaaaaaacg gcgctcaaca gcgtcacatc
      841 gggcaggtgc gagcctccgt atcgggggtg gttggtcatg gccacatcgc cggggccaag
      901 gggcagccgc tcctttacca aacgcgtgca tacgcccaaa ctgcccaaat gcacgggaat
      961 atgcggcgca ttgaccaaca attccgcatc ggcatccaaa atggcgcagg aaaaatcgag
     1021 ccgctctttg atgtttaccg aaaatgccgt gcgttgcaac tgcgccccca tctcctcggc
     1081 gatggcggaa aaacgattgg taaacaactc taattcaatg gcttctccat gcccggcatt
     1141 ggacgcagca gcttcgttgt ggcgaatcac cgcattgccg cccgcctgaa attccaagac
     1201 ccagcctgcc ggaatatagg cggtggagtg ccggttgggc agtaccgccg gaccattcag
     1261 aacggtaccc gccgggggca tttcatttga aaaaaaatga tgggaaatgc cggctccggc
     1321 tctgctgcca cacactaagc gaatgctctc cacctcgatg gcgcggccgg cgggatagtg
     1381 tccaaacaag tgccggtatt ggcgttccat cgctttttgc agatcggaaa tgccgtccca
     1441 acctatttcc acgctgctgc tctgccctct gaaacgcagg cgcaccaatt gatgcagggc
     1501 ttcaatttct tgtgatacaa acccctcggc gttcaaggcg gcctctgctt cggcgcgcaa
     1561 ctgccacagg atgccgggca gattcgggta cgcttcgtcc aacaattcca atacctgcct
     1621 ttcggcaaat cgctctacgc gggcctgacc gatgccataa gcgctcaaga gcccggcgtc
     1681 gtaaggcagc aagacggttg caatgcccag caattccgcc accaggcagg catgaagacc
     1741 gccggcgccg ccaaaagcca gtagcgcata atctcgcgga tcgaaaccac gcgccaccga
     1801 aatgcggcgg atagcggcag ccatgcgttc gtcggctatt tgttcaaagc cgcgcagcaa
     1861 ttcctcttca gaataatgag cgccgccggc ctcgcgtatc tgatttcgga tggccgttaa
     1921 tgcggcttcg gcccgttccc ggaaaatggg aatgcccatc gcttcgggct gcatcttgcc
     1981 cagcaacagg ttcacatcgg tgatgctgag cggccctccg gcgccatagc aggcaggccc
     2041 cggccatgcg cccgcgcttt caggccccac gcagagcttg cgcccgtcga agcggcaaat
     2101 tgaaccgccg ccggccgcca ccgtctcaat atccaacgtg ggactcagca tttcggcctg
     2161 ccccacgcgg gtgaggaaac ggtagtcgta gcgaccatca tagcgggcgc agtcggtgct
     2221 cgtgcccccc atgtctaagg tgaggatgcg ctcaaatccg agctgccggg caatgtgcgc
     2281 cgcgcctact accccgcctg ccggaccact gagaaggctg tctttggggc ggtaatgtac
     2341 agcgcgtacc agaccgccgg cgctggtcat aatgtgcagg gctgcgtcgc cgatgctgcc
     2401 tctgatggaa ctgaggtagg cgtctaaaac cggctccaga tatgcgttga caatggcggt
     2461 ttgcgccctc ggcaacaact gaatggcctg gcttaactcg gaagagcggg aaatgtagcg
     2521 gcagccgcga tttttgagat gtgccgccaa ttgcagttca tgcgcctcat ttcggtagct
     2581 gtgcagcagc gcaatggcga cggagtggcc ggtcggcgtt tgttgagcca aatctgcgag
     2641 atgcggctca tcgggtacga gcagaggcgt cccgttggcg gccaagcgtt cgtccacctc
     2701 aattacccgc tcaaaaagca gttcgggcgg cggcacgtcc aattgaaaca aatgcgggcg
     2761 ctgctgtgtg ccgatggcgg gcagatcggc caatcctttg gtaatgaaca atgtaaccgg
     2821 cgcgcctttg cgttcgagca gggcattggt gcctttggtg gtgcccagac gcatgagcag
     2881 gggcggcaag gaacggtgca ggggactgcg ggtggcgatg cgggccgcca aaacgggggc
     2941 ttcttcgccg gcactgattt caaagcctgc cgctgccgaa acaggaaaat cgcggtccaa
     3001 tcggagcgtg gccttatgcg gttctgtggg cgaaaacagg atttgctgca caagggcgct
     3061 cccgtcggcc ccgtccaaga ggcgaaaccg gtatcccgca aaaatgtctt ccgagcaatt
     3121 ccactgctgt tcaatctgat acaccccggc gccggttcgc tccgtgacga tgcctcgcaa
     3181 ggtgctgttg ctgagtactt tgatgcgaat gattgcacca tccggggcaa tggccatccc
     3241 atcggtgaag gtgccgccgg tatctatcca aatatgccaa tgctgcatga ttgcggttta
     3301 tgctggtcgg aaattctgaa aacggggctg aatttccgct ttttccggca aaccggggtc
     3361 attcaaaaac ccgaaacgat tccaaaaact tgtcggaggc ggcattgagg ctgcgttctc
     3421 ttttcattac cgtttgtacc gagtagtagc ggttgcctac cacataagcg cgggttttga
     3481 tgacggcgcg tttgtctttg tagtccactc gccaggactt gccggggtaa gcgcctaatt
     3541 ttatatcgtt ggaccaagcc acgtcgccgg ccacgctgaa ggcagattct tgtaccgagg
     3601 catcaaaaaa ttcggccacc aaagcgatgg aatcatggtg caggctgccc gccggatagt
     3661 cgcaataact gagcaaatac actacgtttt cggtgctttg tgcggcgtct tcaaaaaaga
     3721 aagtatggta agccagcttt ccgatggccg tctcaatact atcggttttg gtacgcatgg
     3781 cccctggagt aagtacccga aagcgcccct catacgagcg aaattcttcc catttgggtt
     3841 tggcctgagc ctgagtgagg aacggcagaa ggcacaatga caaaatagtt gcgacggcga
     3901 tcaacgggaa gaaaaaacgg tgagaggatg tgttcatttt acaaatcaaa atagtgtaac
     3961 ggaaaagact cctgtttttg caaaatcgtt ggcggaatat tccaacggtt actaaacgca
     4021 agcgcctgaa aaagacgaag cctccgctgt gaggaggctc cgggttggaa tttatgtcaa
     4081 aaacagtgca gtaatatctt ttctcaggcc gtttgccgca gcgtatgcaa agccggcatt
     4141 tccagatggg ccagcgtttc gaagcgatag cccagagcgg agaaatgttt cagcacgcgg
     4201 ggcagggcat aactcatgcg ctcattggcc ttgatgctgt cgtgaaaaac aacgatggaa
     4261 cccggcccgg cattgcgaat cacgttgttg aggcactgct ccggcgagat ggaagtatcg
     4321 aaatcaccgc tgagcacatc ccacattacg atgtggtaat ggcgctgcaa gaactgtgct
     4381 tgcttgggct tcaatcggcc gtaaggcggc ctgaacaatc ctgaatgcgt gagcgcggcg
     4441 caacggcgca catcgtggaa atagtcgagg ttgtcggtat tccaggcatt caggtgcgag
     4501 taggtgtggt tgccgatggc atggccgccg tccaatacct gatggaatac atcggaatgc
     4561 ttacgcacat tgtcgcctac gcagaaaaag gtagccttgg cgttgtagtg ttccaattgt
     4621 tcgagcaccc agggcgtcac ttccggcacg gggccgtcgt cgaaggtgag ataaagaacg
     4681 ggttctttgc cgggcatttt ccaggtaaag gcaggaaagt gcttttggat gaattgggga
     4741 gtcttgacca aatacatagc gttttgattt taagtttgtt cacgggaaaa aagaagacag
     4801 cttgtgcaaa gaacgctttt gattttgcca aagtgtgcga atcggttagc tttgcggcct
     4861 caattgcgca aaagttgttt tcgaaagaat tcgtttgctt tttgtttaca acgtttttca
     4921 gcgaatttcc gctgcgcaaa acggcttttt tttcagaaaa aattgaaaaa attttcatca
     4981 cccccaactg agcatcaaaa atgaacaaaa tcagagtgcg ttttgccccc agccctaccg
     5041 gcgccctgca catcggcggc atccgaacgg ccttgtacaa ctacctgctg gctaaaaaat
     5101 tcaacggtac ttttatcctc agaattgaag atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnggc gattacggcc cttacaggca gtcggaacgc aaggccatct
     5281 atgccgaata cgccggccag atggtgcaaa acggctacgc atactacgct tttgatacgc
     5341 ccgaagaatt ggacgcggcc cgcgaagctg ccaaagaagc cggcaatcat acctttaaat
     5401 acgacgctct ggagagaggc agcctcagaa acagtctcac cctgccggcg gaaacagtga
     5461 gtcaattgct ggaacagggc gcgccctatg tcattcgcct attggtacag cctgggcagg
     5521 acattgttat tcaggatttg gtacgaggcg aggtggtttt tcaaagtgat gagttggacg
     5581 ataaaatcct gctcaaaagc gacggtatgc ccacctacca cttagccaat gtggtggacg
     5641 acacgctgat ggaaattacc catgtcattc gcggtgaaga atggctgccc agcacggcgc
     5701 accacgtttt gttgtatcgc gcattgggcc gcgaggcgca aatgccctcc ttcgcgcacc
     5761 tgccgctcat cctcaaaccc gacggcaaag gcaagctgag caaacgcgac gggcttcgct
     5821 tgggcattcc ggtgtttccc attgcctggc aaggcgaaac gccggaggac tcgttcaagg
     5881 gcttccggga gtttggtttc ctgccgcagg ctatggtcaa ctttatggct ttnnnnnnnn
     5941 nnnnnnnngg gttggaatcc aggcaccgag caggaaattt tcagcatggg ggagctgacg
     6001 gatgcttttt ccattgaaaa gatcggcaag tcgggcgctc gtttcgatta cgacaaagcc
     6061 cgttggttca atcagcaata cctccacgct accgacgacg ctacactggc cggtctcgtg
     6121 gcgcccattg ttcgtgccaa tggttatgac cctacagagg agcaattgca ggcattttgc
     6181 cgcttgatga aagaacgggt gacgctgttg cccgaatttt gggaaaaagg cgcctggttt
     6241 tttgcgggcg ttcaggagta cgatgaggcc aatatcaaaa agcgctggag cgccgaacgg
     6301 agcttacttt ttcatcaact gctcgtcgaa ctggaggctt tgccggaagc ggcttggcag
     6361 ggcgagggcg aagcgttgaa agctgcggtg gaaaccttca tgcaggccaa cggactcaag
     6421 cccggcgatg tgttgccggt gttgcgcata gccttggtag gcaatatgca ggggccggcg
     6481 gtgtttgaac tgatggcggt gtttggacgg gcggaaactt cggcgcgttt ggcgaaagcc
     6541 tgggagcggt tttcaggggt gagtgtgtga ggggtgagca actctcaggg gtgaggggtg
     6601 aggggtgagt gagtgagggg tgagtgagtg aggggtgagt aaccctgctc agtgaagggg
     6661 tgactaacag ggctgaaact tgccgagtca cccattcact acccccg
//
LOCUS       DAORLF010000271         6661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6216_length_6661_cov_1.820163, whole genome shotgun sequence.
ACCESSION   DAORLF010000271 DAORLF010000000
VERSION     DAORLF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_6661_cov_1.820163"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>325)
                     /locus_tag="PK198_18325"
     CDS             complement(<1..>325)
                     /locus_tag="PK198_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF40757.1"
                     /translation="MLIVFLTARDEDYSQIAALDVGGDDYITKPIRPRVFMSRINALL
                     RRFERKDEAEAEQVIRVADLAIDRERVSVMRGEEVIELAKKEFELLSLLASKPGKVFT
                     REEIF"
     assembly_gap    326..374
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<375..566)
                     /locus_tag="PK198_18330"
     CDS             complement(<375..566)
                     /locus_tag="PK198_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF40758.1"
                     /translation="METYKILLVDDEPDILDFLRYNLQKEGFQVFTAGDGVEGLRKAE
                     EIRPHLAILDIMMLKMDGVE"
     assembly_gap    977..992
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1063..>1911
                     /locus_tag="PK198_18335"
     CDS             1063..>1911
                     /locus_tag="PK198_18335"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRF40759.1"
                     /translation="MTTKTVELVMAPPAPHYVGDGFRVHNFIPGAYRLDMQRMSPFIM
                     LDYNSKFYFPPADKPRGVDVHPHRGFETVTIAYKGRVAHHDSAGNSGIIGEGEVQWMT
                     AASGILHKEYHEEAFSAAGGDFQMAQLWVNLPARHKMSPPKYQSITRDQIRRYPLPDD
                     GGHIEIIAGNYEGITGAASTFTPVHLLNAHLKAEATAPFSFPEHYNTALLVVEGSIRI
                     NGREKVPTDNFALFDNDGEHFSITALENSVVLVLSGEPIAEPIAAHGPFVMNTRAELA
                     QAFEDFH"
     assembly_gap    1912..1947
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2392..2431
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2432..3863
                     /locus_tag="PK198_18340"
     CDS             <2432..3863
                     /locus_tag="PK198_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HRF40760.1"
                     /translation="VHARQPFASFAAASNNTDLYVIDPTQATFWKASVNALPLGLREQ
                     LQSTNMNFYQDADTLYIIGGYAFSASANAHKTFTGLISVQVSGLIQAIKNQTDMQPFF
                     KQINDDIFAVTGGQLGKIGHTYYLVGGQRFDGRYNPMGPDHGPGFSQQYTNQIRKFRL
                     HNDGTQLSFTHYEAITDPVHLRRRDYNLLPQIFPDGQQGYTISSGVFQLQADLPFLYP
                     VDITENGYTPITGFNQYLSNYHSAKAALYDSAANRMHSLFFGGISRYYYQNGQLIEDD
                     QVPFVRTISLLSRSADGTLAEYQLPTEMPGLKGAGAEFIVNRNLPHHESEIIQLSAIS
                     SDTILIGHIYGGILSPALNPFSANQTGITTADASVYSVRLIRKNTASAAQQVKGRHSF
                     DFEVFPNPAQENVTLRYDLPYAGKVNYFLYTVDGKILQRGEWERQTSGRNEQSLFVHR
                     SFSAQPLFIALAFDDQFYAVKKIVRE"
     assembly_gap    4119..4152
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4153..>4491)
                     /locus_tag="PK198_18345"
     CDS             complement(<4153..>4491)
                     /locus_tag="PK198_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRF40761.1"
                     /translation="GQLLLSPTRTYLPVIQALLAQLQPHIHGMIHCTGGGQTKVMKFA
                     PGLRIVKDNLFPTPPLFALIQQESQTAWREMYQVFNMGHRLEVYLPEAYAKQVIDIAA
                     KFGIESAVIG"
     assembly_gap    4492..4590
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4591..>4846)
                     /locus_tag="PK198_18350"
     CDS             complement(<4591..>4846)
                     /locus_tag="PK198_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRF40762.1"
                     /translation="ARHGVNIHLAGGETADVGDVVRTIDVGYTAFARMRRHDVIENAL
                     QPGDTIVALASFGQATYEDRYNGGMGSNGLTAARHDVLKND"
     assembly_gap    4847..5052
                     /estimated_length=206
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5053..5251)
                     /locus_tag="PK198_18355"
     CDS             complement(<5053..5251)
                     /locus_tag="PK198_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRF40763.1"
                     /translation="MSEISRYDQRGVSADKGEVHAAVKHLDKGLFPNAFCKILPDVAA
                     GDPLFCNLMHADTAGTKTNLAY"
     gene            5527..6105
                     /locus_tag="PK198_18360"
     CDS             5527..6105
                     /locus_tag="PK198_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40764.1"
                     /translation="MNHQTDESTSEQTSRTGGAFKWIKKAALGVAALAIGFAAYQLAE
                     PDYISASAKDAVPTIELGAFPIIKPTMRWGFAIDTFTVTEYKISGGQSLSDMLMARQL
                     SQEAIQAIVKNTEGIFDFRLLQAGKSYNILNHGPSGEQMYMVYEPSVYEYVLFSLGSD
                     YKVERIRGGHAGRNRPFCGGVHPVGSNGRPGP"
     gene            6029..>6661
                     /locus_tag="PK198_18365"
     CDS             6029..>6661
                     /locus_tag="PK198_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF40765.1"
                     /translation="MDTQVETGHFVVESTLWEAMVGQGHSYELADRLEDALKWSIDFH
                     RIQENDEFKVVFENKYIEGKSVGAGKVEGAYHKRSDKEVYAVYYENGEHKGYYDLEGR
                     PLKTAFLKAPLRFSRISSRYNLNRFHPVLKRRRAHLGTDYAAPHGTEILAVGDGVVSQ
                     AHYSGGNGNFVRIKHDKVYETQYLHMSRFAKGIRPGTRVSQGQVIGYVGST"
BASE COUNT         1438 a         1853 c         1539 g         1351 t
ORIGIN      
        1 ctgaaaatct cctcgcgggt aaataccttg cccggcttgg aggccaataa agacaggagt
       61 tcaaactctt ttttggccaa ctcaatcacc tcctctcccc gcatcacaga cacccgctcg
      121 cggtctatcg ccaagtcggc cacgcgaatc acctgctcgg cttctgcctc gtctttgcgt
      181 tcaaaacggc gcaacaaagc gttgatgcgg ctcataaaaa cccggggccg tatgggcttg
      241 gtgatgtaat cgtcgccccc cacatccaag gccgctattt gggagtagtc ttcgtcgcgg
      301 gccgtgagga agacgatgag catatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnttccac gccgtccatc ttgagcatca taatgtctaa aatcgcgaga
      421 tgcggtcgta tttcttccgc ttttcggagg ccctccactc cgtctcctgc cgtaaatacc
      481 tgaaaacctt ctttttgcaa gttgtatcgc aggaaatcca gtatatcagg ctcgtcatcc
      541 accaacagga ttttgtatgt ctccatagta agctcaggct tgtgttaggg caaagttaag
      601 cgcttattat tcgggcaagg ttgtaaccct actttaattt atcgttaact ctggattttg
      661 ggggcgggtt tcatccgccg aaccacgcca caagcgtggg aaggcgggtt tcgggttcaa
      721 ggtttcgggt ttcgggttgc taccgccgac tgcctaccgc atactggttt tcgggttcca
      781 ggtttcgggt tccaggctcc ctctcgcgtt cgcggaaatt cgcgcccttc gccatttttc
      841 gcgttaaaaa aaacgcacca caccacccgc acagcccctc tccgcaagca gataaacttt
      901 ctccctctcc gctggaagat aaactgcgca ccgcgcaagg cgcgggcagc agcttacgcc
      961 aaaggcagat aaccccnnnn nnnnnnnnnn nncgcaggcg ataaaaccct accccccatt
     1021 cacttgttac gccctcgttc atcatccaaa caaccaccca aaatgacaac caaaaccgta
     1081 gaattggtca tggcgccacc cgccccacac tacgtcggcg acggcttccg cgtacacaat
     1141 ttcatacccg gcgcctaccg ccttgacatg cagcggatga gccccttcat catgctcgac
     1201 tacaactcga aattctactt cccgcctgcc gacaaaccgc gcggcgtgga cgtccacccc
     1261 caccgcggct tcgaaaccgt caccattgcc tataaaggcc gcgtagccca ccacgacagc
     1321 gccggcaaca gcggcatcat cggcgagggc gaagtacaat ggatgactgc ggcctccggc
     1381 atcctccaca aagagtacca cgaggaggcg ttcagcgctg ccggcggcga tttccagatg
     1441 gcgcaactgt gggtcaacct acccgcccgg cacaaaatgt cgcccccgaa ataccagagc
     1501 atcacccgcg atcaaatccg ccgatacccc ctccccgacg acggcggcca catcgaaatc
     1561 attgcaggca attatgaagg gatcaccggg gccgcttcca cgttcacgcc cgtccacctg
     1621 ctcaacgcac acctcaaggc agaagccacc gccccatttt ccttccccga acactacaac
     1681 acagccctcc tcgtagtcga aggcagcatc cgcatcaatg gccgcgaaaa agtacccacc
     1741 gacaacttcg ccctcttcga caacgacggc gaacacttca gcatcacagc cctcgaaaac
     1801 tcagtagtgc tcgtcctcag cggcgaaccc atcgccgaac ccatcgccgc gcatggcccc
     1861 ttcgtcatga acacccgcgc cgaactcgca caggccttcg aagacttcca tnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt agtccttcgg cagtttatct cttttttttg
     1981 gggcacccgc ccgctctgat aaatagctgt tggcttttcc ggcaagaaac agccaaagcc
     2041 aacagctcta tccatgcggc gggcgccggg ccatccgttt caagcgcttc gtacctccgc
     2101 gctttccact gctgtccctg ctgcggcaaa cctgcattcc tcacccctgc acccctcacc
     2161 cctgattatg gtgacattca tcacttatct ttcaccccga caggccgtaa ctttggcccc
     2221 gctcatcaat ccaaaccacc atgaaaaaca tcgccgtagc cattgcgctg ctctgttttg
     2281 cactcacgct gtccgctcag gaagcctttg acttcgaagt tcaactttct cccgtagctg
     2341 ttcctaatct gccgggactg cactcctacg ccttcgccga gcacaatggc cnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgttcatgc ccggcagccc ttcgcctcct
     2461 tcgccgccgc ctccaacaat accgacctct acgtcattga cccgacgcag gctacctttt
     2521 ggaaagcctc ggtaaacgcg ctgcccctcg gcctgcgcga gcagttgcaa tccaccaaca
     2581 tgaattttta tcaggatgca gatacgctct acatcatcgg gggctatgcg ttttcggctt
     2641 cggccaatgc gcacaaaacc ttcaccgggc ttatttctgt gcaggtttcc ggcttgatac
     2701 aggccatcaa aaaccaaacc gatatgcagc cgttttttaa acaaatcaac gacgacatct
     2761 ttgccgtcac cggcgggcaa ttgggcaaaa taggccatac ttactacttg gtaggcggcc
     2821 agcgcttcga cgggcgctac aaccccatgg gacccgatca cggccccggt ttctcgcagc
     2881 aatacaccaa tcagattcgc aaatttcgcc tgcacaacga cggcacgcaa ctctccttta
     2941 cccactacga agccatcacc gaccccgtac accttcgccg ccgagactac aacctcctgc
     3001 cgcaaatctt ccccgatggg caacagggct acaccatctc ttccggcgtt ttccaactcc
     3061 aggccgacct gcccttccta tatcccgttg acatcaccga aaacggatac acgcccatca
     3121 ccggcttcaa ccagtacctc tccaactacc acagtgccaa agctgccctg tacgacagcg
     3181 cggccaacag aatgcactcc ctctttttcg gcggcatcag ccggtattac tatcaaaacg
     3241 gccaactcat cgaagacgac caggtgcctt ttgtgcgcac catcagcctg ctcagccgca
     3301 gcgccgacgg tactttagca gaataccaat tgcctaccga aatgcctggc ctcaaaggcg
     3361 cgggagcaga attcatcgtc aaccggaacc ttccgcacca cgagtcggaa atcattcagc
     3421 tatcggctat ctcctccgat accattttga tcgggcacat ttatggcggc attctcagtc
     3481 cggcgctcaa tcctttcagc gccaaccaaa cgggcattac cacagccgat gcctcggtgt
     3541 acagtgtacg cctcattcgg aaaaacactg ccagtgcagc acagcaggta aaaggacgcc
     3601 attcctttga ttttgaggta tttcccaatc cggcgcagga aaacgtaacc ctgcgctacg
     3661 atttgcccta cgccggaaag gtgaactact tcctatacac cgtggacggg aaaatactgc
     3721 aacgcggcga atgggagcgc cagacaagcg gtcgcaacga acagtccctg ttcgtgcatc
     3781 gcagtttttc cgcgcagccg ctgttcatcg cattggcctt tgacgaccaa ttttatgcag
     3841 tgaagaagat tgtgcgagag tagagacgtt gcacgcaccg tcttgtgcgc cagggataga
     3901 aacggaatgg cggcggtcgg ggatagagct gttagctttt ccggcaatgg ccatgccaaa
     3961 tctaacagct ctgcacggag gccgccatgc agtggatagc ccgcccgccg gagagcattt
     4021 acaggtggcg ggggcgcccc aaaaatatca gaagcaggta ggttgaacga tcccttcctt
     4081 acgcccattc aaaacggcca tgttcggtat ccagcacgnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnggccaatc acggcgcttt cgatgccaaa ttttgcagca atgtcaatca
     4201 cctgtttggc gtaagcctcc ggcaaataga cttcgagacg atgccccata ttgaatacct
     4261 gatacatttc gcgccaggcc gtttgagatt cctgttggat gagtgcaaaa agcggcgggg
     4321 tagggaagag gttgtctttg acgatgcgca gtccgggagc gaatttcatc actttggttt
     4381 ggccgccgcc ggtgcagtgg atcatgccgt gaatatgggg ttgcagttgt gccagaagtg
     4441 cttgtatgac aggcaggtag gtgcgggtag gcgagagcag cagttggccc annnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtcgtttttc agcacatcgt ggcgggctgc
     4621 ggtgaggccg ttgctgccca tgccgccgtt gtagcggtct tcgtaagtgg cctgaccaaa
     4681 ggaggccagc gctacgatgg tatcgccggg ttggagtgca ttttcgatga cgtcatggcg
     4741 gcgcatgcgt gcaaaggccg tgtagcctac gtctatggtg cgcaccacat cgcccacgtc
     4801 ggcagtttcg ccgcccgcga ggtgaatgtt tacgccgtgc cgggccnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnggtaggcg aggttggttt tggtgccggc tgtatcggcg tgcatgaggt
     5101 tgcaaaagag gggatcgccg gcggccacat cgggcaggat tttgcaaaaa gcattgggaa
     5161 ataagccttt atccaagtgt ttgacggcgg cgtgtacttc gcctttatcg gcagagacgc
     5221 cgcgctggtc gtagcgggag atttcggaca tgcgagttga aattgtgggg gcaaaagtac
     5281 acagggcagc cgttatgaag gcataaaaac gcgattgggg aagatttaac aaaaaaaaat
     5341 tacctcccaa agcgatcctc gtatatattt gcgcctcgtc ttcgcagacg atacgacatt
     5401 tagtcccgtg aacatcaggc aggttactct caggagggcc ggccgctgaa tgtacggcaa
     5461 accaatcgca caatgttctt ccttacgata ggtttgcgtt gttttgaacg caaaaaataa
     5521 ccgatagtga atcatcaaac cgacgaatca acttcagaac aaacgtcccg cacaggcggc
     5581 gctttcaagt ggatcaaaaa agcagcttta ggtgtagcag cccttgccat aggctttgca
     5641 gcgtaccaat tggctgagcc tgactacatc agtgcttctg ccaaagatgc tgttcctacc
     5701 attgaattag gagcgttccc catcatcaag cccaccatgc gctggggctt tgcgatagat
     5761 acctttaccg ttacggaata caaaatcagc ggcgggcaat cgctgtcgga tatgctgatg
     5821 gcccggcaat tgagccagga ggctattcag gcgattgtga aaaacacgga aggcattttt
     5881 gattttcgtc ttctgcaggc cggcaaatct tacaacattc tgaatcacgg cccttccggc
     5941 gaacagatgt acatggtgta tgagcccagc gtgtatgagt atgtgctgtt cagccttggc
     6001 agcgattaca aagtagaacg catacgaggt ggacacgcag gtagaaaccg gccattttgt
     6061 ggtggagtcc accctgtggg aagcaatggt aggccagggc catagctacg aattggccga
     6121 ccggttggaa gacgccctca agtggtctat tgatttccat cggattcagg aaaacgacga
     6181 gttcaaagtt gtttttgaaa ataaatacat cgagggcaaa tctgtgggcg cgggcaaggt
     6241 agaaggcgcg tatcacaagc gcagcgataa agaagtgtat gctgtttatt atgaaaatgg
     6301 cgaacacaaa ggatattacg acctcgaagg ccgccccttg aaaacggcct tcctgaaagc
     6361 ccccttgcgt ttttcgcgta tttcttcgag gtacaatctc aatcgttttc accctgtcct
     6421 gaaaaggcgg cgcgcccact tgggtaccga ttacgcagcc ccgcacggta cagaaattct
     6481 ggcggtcggc gacggggtag tttcgcaggc gcactactcc ggcggcaacg gcaattttgt
     6541 gcgcatcaag cacgacaaag tgtatgaaac ccagtacctg cacatgtctc gttttgccaa
     6601 aggcatccgt ccaggcacgc gggtatctca ggggcaggtt attggatatg tgggttctac
     6661 c
//
LOCUS       DAORLF010000272         6647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6233_length_6647_cov_2.311742, whole genome shotgun sequence.
ACCESSION   DAORLF010000272 DAORLF010000000
VERSION     DAORLF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_6647_cov_2.311742"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK198_18370"
     CDS             complement(<1..267)
                     /locus_tag="PK198_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HRF40766.1"
                     /translation="MTTQQHFQYLLRLGDNALILGQRLSEWCGHGPVLEQDIAITNIA
                     LDLVGQARSILEYAGQVEGAGRSEDDLAYHRNAHQFFNILLVEQP"
     gene            complement(348..1268)
                     /locus_tag="PK198_18375"
     CDS             complement(348..1268)
                     /locus_tag="PK198_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRF40767.1"
                     /translation="MKYSFLEIAPDVAEALQNKRPVVALESTIIAHGMSYPDNVENAL
                     RCEAIVRENGALPATIAIIDGRFKVGLSTDEIERLGREGQKISKTSRRDIAFILARKA
                     CGATTVASTMIAARLAGIPIFATGGIGGVHRGAAETMDISADLQELARTNVAVVCAGA
                     KAVLNLELTLEYLETYGVPVVGFQTEEFPAFYTRRSGLPVDYRIDTPEELAFALRVKW
                     DTGVQGGVVIANPIPKSHAMNRESVEQAIEQAIHDADAQEIRGKALTPFLLARVEQLT
                     SGVSLLANKALVQNNAALAAKVAAAYSALD"
     gene            1408..1827
                     /locus_tag="PK198_18380"
     CDS             1408..1827
                     /locus_tag="PK198_18380"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRF40768.1"
                     /translation="MSKCIFCEIAAGRIPASIVYEDNMTVAFMDIHPVNPGHVLVMPR
                     RHFETLSDMDETTGMHLFQIVMRVEQALRNMEGLRCEGTHLLQNNNTAAGQDVPHVHI
                     HIIPRFADDGVQFHLRTGPTPDREALNALADAIELAM"
     gene            1907..2389
                     /gene="purE"
                     /locus_tag="PK198_18385"
     CDS             1907..2389
                     /gene="purE"
                     /locus_tag="PK198_18385"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRF40769.1"
                     /translation="MVSIIMGSDSDLPVMQQAAEMLRHFDIPYEMSIVSAHRTPERMV
                     EFAKNAHLRGLRVIVAGAGGAAHLPGMVASLTPLPVIGVPVKSSNSLDGWDSLLSIVQ
                     MPNGVPVATVAVNAAKNAGILAAQILAASDAGLLKKISDYKDQMRSEVEAKAAKMEGR
                     "
     gene            2415..3227
                     /locus_tag="PK198_18390"
     CDS             2415..3227
                     /locus_tag="PK198_18390"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF40770.1"
                     /translation="MTKVITSVQNPLIKNIVLLTEKARERKAQGLFVAEGLRETAMAL
                     AAGYEAEAILYDPARTGFDDLLPFQKMAPDALTEVSRAVFEKTAYRSGVPNVVALLRT
                     RPLVLDNIALPRCPLILVLESVEKPGNLGAILRTADAAGVDAVILCDPLTDWHNPNVI
                     RASLGAVFTVPVATTDSEQVLAWLRRQGVKLAVTHLEASHPHYLTDFTQPTAIVMGAE
                     ATGISAFWIEAADERVIIPMQGRVDSLNVSASTAVLLFEARRQRGESASQFI"
     gene            3255..4568
                     /locus_tag="PK198_18395"
     CDS             3255..4568
                     /locus_tag="PK198_18395"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF40771.1"
                     /translation="MLLKNDRRTIRAWTMYDWANSSYALVINSAIFPAYYNSVTRQGE
                     NNRVSFLGFDVENTALYSIALGLSFGIVALLSPLLSSISDYAGNQRSFMQFFCYLGAL
                     ACMALFFFTGGDQLGIGIAGLMLATIGYSGSIVFYNAYLPAIADEAIQDRVSAQGYAM
                     GYMGATTLLILNLAFILNEKSLGIEDGTLLPRLAFLSTGLWWMGFAQITFRRLPHGIK
                     AARPRNGGRFLLHGYEELLNVWRRIRSERVLRNFLPAFFFYIMGVQTVMFMAASFGEK
                     EIKLPMAALIATVLSLEYIAIGGAYLFAALSKRFGNVPALMLAVGLWLCICIGAYFIY
                     DVWSFFVCAFFIGIVMGGIQSLSRSTYAKMIPATENNAGFFSFYDVCEKVAIMLGLLM
                     FGTLDQMTGSMRTSIFALGIWFAVGLVFLWRVHRLRMQTFNKTME"
     gene            4654..4914
                     /locus_tag="PK198_18400"
     CDS             4654..4914
                     /locus_tag="PK198_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40772.1"
                     /translation="MSFVFVIITLILLLILLGFSVERMLSRQILPQPLLLSTSLVAGL
                     WSGLMVVSWDMLGAAVLATGATALMLVLLRWYNQRMKHKNRI"
     gene            5026..>6647
                     /locus_tag="PK198_18405"
     CDS             5026..>6647
                     /locus_tag="PK198_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRF40773.1"
                     /translation="MIEFYIVIVVLLLILAATDLMVGVANDAVNFLNSAIGAKVAPRH
                     IILLVASLGVFVGATFSSGLMEVARKGIFNPELFVFADVMVIFLAVMITDIMLLDLFN
                     TFGLPTSTTVSLIFELLGAAVAVALIKIVKSGALLSTLPDYINTSSATEIVSAIFSSV
                     AISFVVGSLVQYFSRLLFTFHYERRMKWVGGIWSGAALALLTYFLLFKGIKGASFVSE
                     GFVKWVGTHTTQLLVISCVFWTLIMQTLITVFRVNVLKVVVLFGTFSLAMAFAGNDLV
                     NFIGAPIAGLESFMAWRSSGIPADEFNMGVLGKQVKTNTVLLLIAGAIMVLTLWLSKK
                     ARSVTDTEVSLGRQDEGIENFAPNFLARGIVNVFGVVGIGLRRIFPESWRTKMEQSFQ
                     PVSLASMTVADRPSFDLVRASVNLTAASMLIAFATSLKLPLSTTYVSFMVAMGTSLAD
                     KAWGRDSAVYRVAGVLNVIAGWFFTAIIAFTVAATVAWFIHTFGPWAVGVMVLALIYF
                     FYHTYTVHKKREKAKAEIQEAVQESVAIPATT"
BASE COUNT         1390 a         1733 c         1834 g         1690 t
ORIGIN      
        1 gggttgttct accagcaata tgttgaaaaa ctgatgggca ttgcgatggt aagccagatc
       61 gtcttccgag cggcccgcgc cttctacctg cccggcatat tccaatattg agcgcgcctg
      121 ccccaccaga tcgagggcga tgttggtgat ggcgatatct tgctccagca caggcccgtg
      181 gccgcaccat tcggagagac gttggccaag tatgagggca ttgtcgccga gacggaggag
      241 gtattggaag tgttgttgag ttgtcataag actattggtg ggaaaaatgg aattccttat
      301 agcaaaacaa atgtagcgct tcggctcatc atttcaaaat gcggccctta atccaatgcg
      361 gaataagccg ccgccacctt tgccgccagg gctgcattgt tttgcacgag cgctttattg
      421 gccagcaggc tcaccccgga ggtgagttgt tccaccctgg ccagcaagaa cggagtgagc
      481 gcttttcctc tgatttcctg cgcatcggca tcgtgtatgg cctgctctat ggcctgctct
      541 accgattcgc ggttcatggc gtgcgatttg ggaatgggat tggcaatcac tacgccgcct
      601 tgcacaccgg tgtcccattt cacgcggagg gcaaaagcca gttcttcggg cgtatctatg
      661 cgatagtcca cgggcagccc gctgcgccgg gtgtagaacg ccggaaactc ttctgtttgg
      721 aaccccacga ccggaacgcc gtaagtttcg agatattcca gtgtgagttc gaggttgagc
      781 accgctttgg cgcctgcgca aaccaccgcc acattggtgc gggccagttc ctgcaaatcg
      841 gcggaaatat ccatcgtttc tgcggccccg cgatgcacgc cgccaatgcc gccggtggca
      901 aaaatgggga tgccggccaa ccgtgcggct atcatggtag aggctacggt tgtggcgcca
      961 caggctttgc gggcgaggat gaaggcaatg tcccggcggc tggttttgga aattttttgc
     1021 ccctcgcggc ccaagcgctc tatctcatct gtgctgaggc ccactttaaa ccggccgtct
     1081 atgatggcaa tggtggccgg cagcgctccg ttttcgcgta cgatggcctc gcaacgcagg
     1141 gcattttcca cattatcggg gtaagacata ccgtgcgcaa tgatggtgga ctccaatgcc
     1201 actacagggc gcttgttttg caaggcctcg gcaacatcgg gagcaatttc gagaaatgaa
     1261 tatttcataa caattagtgg gttgggtgct gcaaaagtag gaatttttat gcaaacaact
     1321 acttttgtac cggcccggtg cattgaatgc cgtaccttga gggccgaccc ggcacccgcc
     1381 gttcttcaaa caaaagcgtt catccgtatg agtaagtgca ttttttgcga aatcgcagcc
     1441 ggcagaattc ccgccagcat tgtttatgaa gacaacatga ccgtcgcatt catggacatt
     1501 catcccgtca atccggggca tgtattggtg atgccccgac ggcattttga aaccctgagc
     1561 gatatggacg aaaccaccgg catgcacttg ttccagattg ttatgcgcgt agaacaggca
     1621 ctccgcaata tggaaggctt gcgctgcgaa ggcacccatt tgctgcaaaa caacaacact
     1681 gcagccgggc aggatgtgcc gcatgtacac atacacatca ttcctcgttt tgccgacgat
     1741 ggggtccaat tccatctgag aaccggcccc actcccgacc gggaagccct gaatgcgctg
     1801 gcagatgcga ttgaactggc gatgtaggaa acatagagca tacaagcgaa gcgcaatccc
     1861 ggatagcctg gaaatcggga gagcatagag aaatcaatac tcaattatgg ttagtatcat
     1921 catggggtcg gattcagacc tgccggttat gcagcaggcg gctgaaatgc tccggcactt
     1981 cgatatccct tatgaaatga gcatcgtgtc ggcgcaccgc acaccggaac gcatggtgga
     2041 atttgccaaa aacgcccatc tgcggggctt gcgcgtcatt gtggcgggag ccggcggcgc
     2101 ggcgcatttg ccgggcatgg tggcttcact cactcccctg ccggtgatcg gagtgccggt
     2161 aaaatcgagc aattcattag acggctggga ttcgctactt tccattgtac aaatgcccaa
     2221 cggagtgccg gtggccactg tggccgtcaa tgccgctaaa aacgcgggta ttctggcagc
     2281 gcaaatattg gccgcgtccg atgccgggct gttgaaaaaa atatcggact acaaagatca
     2341 aatgcgctcg gaggtggagg ccaaagcggc gaagatggaa gggaggtagc aagcaaatat
     2401 cagcacaagt agccatgacg aaagtaatta ccagcgtcca gaatccgctt ataaaaaaca
     2461 tcgtgttgct caccgaaaaa gcccgggaac gcaaggcgca gggtttgttt gtggcggagg
     2521 ggctgcgcga aaccgcgatg gcgctggctg ccggttatga ggcggaggct attttgtacg
     2581 acccggcccg caccggtttt gacgatttgc tgcctttcca aaaaatggcc cctgacgctt
     2641 tgacggaagt gagccgtgcg gtatttgaaa aaacagctta ccgaagcggc gtgcccaatg
     2701 tggtggcgct gttgcgaacg cgcccgctcg tattggacaa cattgcgctg cctcgctgcc
     2761 cgctgatact cgtgttggaa agtgtggaaa aacccggcaa tctgggggcc atcctgcgca
     2821 cagccgatgc cgccggcgtg gatgccgtca ttctgtgcga tccgctcacc gactggcaca
     2881 atcccaacgt catcagggca tctttggggg cggtgtttac cgtgccggtg gctaccaccg
     2941 attctgaaca ggtgcttgcc tggctgcgac ggcagggcgt aaagttggca gtcacgcatc
     3001 tggaggcttc gcacccgcat tatctcactg attttaccca accaaccgca atcgtcatgg
     3061 gcgccgaagc tacgggcatt tctgcattct ggatagaggc cgccgacgag cgcgtcatca
     3121 ttcccatgca ggggcgcgtg gattcgctca acgtgtcggc ttccacagca gtgttgcttt
     3181 ttgaagcaag aaggcagagg ggggaatctg cttcgcagtt tatctgactg tgtttaattc
     3241 ttcatttcat cgccatgctc cttaaaaacg accgacgcac catcagagcc tggaccatgt
     3301 acgactgggc caactcttcg tatgccttgg tcatcaactc ggccatattt ccggcgtatt
     3361 acaacagcgt cacccggcag ggcgaaaaca accgggtttc ctttctgggc tttgatgttg
     3421 aaaacacggc gttgtattcc attgcgctgg ggctgtcatt cggcatagtg gccttgttgt
     3481 cgccgctgtt gtcttccatt tccgattatg cgggcaatca gcggtcgttt atgcagtttt
     3541 tctgctatct gggagccttg gcctgcatgg ccttgttttt ttttacgggc ggcgaccaac
     3601 tcggtatcgg catcgccggc ctgatgctgg ccaccatcgg ctattcgggc agcatcgttt
     3661 tttacaacgc ctacctgccg gccattgccg acgaggccat acaagaccgg gtgagtgccc
     3721 agggttatgc catgggctac atgggggcaa ctaccctgct gattttgaat ctggctttta
     3781 ttttaaatga aaagtcactg ggcattgagg acggtaccct gctgccccgg ctggcattcc
     3841 tgagcaccgg cctgtggtgg atggggtttg cacaaattac ttttcgccga ttgccgcacg
     3901 gcatcaaggc cgcaagacct cgaaacggcg ggcgattttt gctgcacggc tacgaggaac
     3961 tgctgaatgt atggcgccgc attcgctccg aacgggtgct gcgcaacttc ctgccggcct
     4021 tcttttttta catcatgggc gtgcagacag tcatgtttat ggcggcctcc tttggagaaa
     4081 aagagatcaa attgccgatg gctgcgctca ttgccacagt gttgagctta gagtacattg
     4141 ccatcggcgg cgcgtatttg tttgcagcct tgtccaaacg attcggcaat gtgcccgccc
     4201 tgatgctggc tgttgggctt tggctgtgca tttgtatcgg agcgtatttc atttacgatg
     4261 tctggtcgtt ttttgtctgc gcttttttca tcggcattgt gatgggcggc atacaatccc
     4321 tgtcgcgctc cacctacgcc aagatgattc ccgcaactga aaacaacgcg ggatttttca
     4381 gcttttacga tgtgtgcgaa aaagtggcca tcatgctggg gctgctcatg ttcggcacat
     4441 tggaccaaat gaccggcagc atgcgcactt ccattttcgc gttgggaata tggtttgccg
     4501 tcggccttgt ttttttgtgg cgtgtgcata ggttgagaat gcagacattt aacaaaacta
     4561 tggagtaaat ttgctcgccg ctttgtgttc cccttcccgc aaagcaacgt ctaaagccca
     4621 aatgttttga attacctcaa aaaacggaag tgcatgagct ttgtgttcgt cattattact
     4681 ttgatattgc tgctgatatt gctggggttt tcagtggagc gtatgttgtc gaggcagatt
     4741 ttgccacaac cgctgctctt gtctaccagt ttggttgcgg ggctgtggag cggcctgatg
     4801 gttgtttcct gggatatgtt gggcgccgcc gtattggcaa cgggcgctac ggctctgatg
     4861 cttgtactgt tgaggtggta taaccagcgg atgaagcaca aaaatcggat ttagaacagg
     4921 cttaggttaa ctgtgtatta attttttgca cgtttctgcc tacgctccaa cgggagtagc
     4981 tatctttcgg acttgtatat aaaggccctc accaatccgg catgtatgat tgagttttac
     5041 attgttatcg tagtactgtt gctcatttta gcggcaaccg acctgatggt aggcgtagcc
     5101 aatgacgccg tgaactttct caattctgcc atcggcgcca aagtagcacc ccgtcacatc
     5161 atcctcctcg tagccagcct tggcgttttt gtgggcgcca cgttctccag cggcctcatg
     5221 gaggtggccc gcaaaggcat tttcaaccct gagcttttcg tttttgccga tgtgatggtc
     5281 atttttctgg ccgtgatgat taccgacatc atgctgctcg acctgttcaa taccttcggg
     5341 ctgcctacct ctactaccgt atcgcttatt ttcgaattgc tgggcgcggc ggtagcggta
     5401 gcgctcataa aaatagtaaa gtcgggggca ttgctttcta ccctgcctga ttatatcaac
     5461 acatccagcg ccaccgaaat tgtttcggcc attttttctt cggtggccat atcctttgtg
     5521 gtaggcagcc tagtgcaata tttctcacgc ctgctgttta ccttccacta cgagcggcga
     5581 atgaaatggg tgggcggcat ctggtcgggt gcagcactgg cacttctgac ctactttttg
     5641 ctttttaagg gcatcaaagg ggcttctttc gtttcggaag gattcgtcaa atgggtgggt
     5701 actcacacga cccagttact ggtgatatcc tgtgtctttt ggacgctgat catgcaaacg
     5761 ctcattacgg tatttcgggt gaatgtactg aaagtggtag tgctgtttgg tactttttcg
     5821 ttggccatgg cctttgccgg caatgatttg gtcaacttca tcggggcgcc catagcgggc
     5881 ctggaatcct tcatggcctg gcgttcgtcg ggaataccgg cagacgaatt caatatgggc
     5941 gtacttggca aacaggtaaa aaccaacacc gtgctgttgc tcattgccgg cgccatcatg
     6001 gtactgacct tgtggctttc caaaaaagcg cgctctgtga cagatacaga agtgagcctc
     6061 ggccggcagg atgagggtat agaaaatttt gcccccaatt ttctggcacg cggcattgtc
     6121 aatgttttcg gtgtagtggg catcggcctg cgccgcatct tccccgaaag ctggcgcacc
     6181 aaaatggaac aaagttttca gccggtcagc ctcgcctcta tgaccgttgc cgaccgcccg
     6241 tcttttgact tggtgcgggc ttccgtcaat ctcaccgcag ccagtatgct catcgctttt
     6301 gccacctcgc tcaaactgcc gctttccact acttatgtgt catttatggt ggctatgggt
     6361 acttcgctgg ccgacaaggc ctggggccgc gacagcgccg tgtatcgcgt agccggcgtg
     6421 ctgaatgtga ttgccggttg gttttttacg gccatcattg ccttcacagt ggcagctacg
     6481 gtagcttggt tcatacacac cttcggcccc tgggcggtag gcgtgatggt actggcgctc
     6541 atttatttct tctaccatac ctataccgtg cataaaaagc gggaaaaggc caaagccgaa
     6601 attcaggaag ctgtgcagga atctgtagcc ataccggcca ctacctt
//
LOCUS       DAORLF010000273         6636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6245_length_6636_cov_2.095274, whole genome shotgun sequence.
ACCESSION   DAORLF010000273 DAORLF010000000
VERSION     DAORLF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_6636_cov_2.095274"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1824
                     /gene="dnaB"
                     /locus_tag="PK198_18410"
     CDS             286..1824
                     /gene="dnaB"
                     /locus_tag="PK198_18410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRF40774.1"
                     /translation="MAEQKDSTNKPVAGAQQRRPGVPRKGEDLSNYVFGKVQPQALPL
                     EEAVLGAVMLDKNALTVILDILQPDSFYLEANQHIYRAMRRLFDRSQPIDLLTVMEEL
                     KKSGDLEAVGGPAYLAELTHRVASAANIEYHARIISQKYIQRELIRVSTKTIRDAFED
                     TSDVFELLDEAEQGLYNIAQNNMSRGYESTGTLAGKMLKQIEELKGKKDGLTGVPTGF
                     TDLDRLTSGWQPSDLIIVAARPGMGKTGFVLSMTRNAAIDFKKPVALFSLEMASLQLV
                     LRLVSMEAEISGSKLRNGQLEEEEWKRLHKSVERISEAPIYIDDTAGINIFELRAKAR
                     RMKLQHDIQLIIIDYLQLMSGGPDSGKGNREQEISSISRSLKALAKELNVPVIALSQL
                     SRAVETRGGTKRPQLSDLRESGAIEQDADIVSFIYRPDYYQIMEDENGQSLKGVAEII
                     IAKHRNGALETVKLRFTDKFARFSDLDEFNFDALPSGVFQDPTPANVIIRPSRMNDDE
                     DIPF"
     gene            complement(1821..2159)
                     /locus_tag="PK198_18415"
     CDS             complement(1821..2159)
                     /locus_tag="PK198_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40775.1"
                     /translation="MEEEGSDLQQLTALVAQDFDPVPLQEAVSEERLLELLSEQVAWL
                     IEHRLEYLMSLMYRLDVDERKVDRALSPAAEEPAHTALAKLILDRQRQRAFTKKHYRQ
                     QFPENWDWDD"
     gene            complement(2167..>3167)
                     /locus_tag="PK198_18420"
     CDS             complement(2167..>3167)
                     /locus_tag="PK198_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40776.1"
                     /translation="HAAMATQQVLYLREKFESMKSAKYFFVALSVFIAADCGNPAPDN
                     SGATTTTDTTATTPPPPRATHDCKVAGKMLDEGASIWIRSQEILVAVTADSSTFDADY
                     GDSYRVLEVYDTRNCSLLERKVLPVNVSPDFPYYVAEISYNKVHEMAAVRGFNSIYLY
                     DAENRRWLPELKPKFASARSGADAQSGMIQRLELWEDYLIGYAQDFGAFAFTLADKAK
                     PAPVLALAEYKPASGAAQSLFLLPVQGAFQAISPSYNADAGTFSVNPMLDAPIALKTD
                     IQKSAANNRYIVLRENTSANTAYAFDLAKNERVALPAEVAAQPTQEVLAWIRANRK"
     assembly_gap    3168..3183
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3459..3481
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3549..3770
                     /locus_tag="PK198_18425"
     CDS             3549..3770
                     /locus_tag="PK198_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40777.1"
                     /translation="MYPNILSNGQKELLPILGSLQREFYLVGGTAIALQIGHRQSIDY
                     SEEVTFLPSFEVSNDEVKRFLTEVASRSI"
     assembly_gap    3782..3867
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3868..5193)
                     /locus_tag="PK198_18430"
     CDS             complement(<3868..5193)
                     /locus_tag="PK198_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF40778.1"
                     /translation="MAQEWSRRDLLKTMGALSVGAAMSNQLWAGPMQEADYTWDYTQL
                     PKAGRKITAITLGAGSRGNVYGNYALKHPDELDIIGVAEPIPLRNERYSKNHNIIDAN
                     RFVTWEHVFTRPKFADAVIITTPDGLHYGPAMKALEMGYDLLLEKPIAQSWKECNDIL
                     QQAKKYKRIVAVCHVLRYSPYYRKVKEVIDSGVLGSIVSVQHLEPIQHVHMSHSYVRG
                     NWRREEDTNPIILAKSCHDLDILRWWLGKNCRYISSFGSLKWFKSENAPAGSTARCTD
                     GCAVEAQCPYSALRIYHKNRTWLHHFDLPAEKELQGDAIMKYLKEGQYGRCVYRCDNN
                     VPDHQIVSMEFEDQITVNFSMEAFTNYAGRRTRIMGSMGDLVGDENELYIADFRNGEI
                     TKWNVRDHADISSGHGGGDWGLARDWVRAVEQQNASLLTSSLDASMESHL"
     gene            complement(5342..6079)
                     /locus_tag="PK198_18435"
     CDS             complement(5342..6079)
                     /locus_tag="PK198_18435"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF40779.1"
                     /translation="MKNFKWILGLLAVTVLAPGCFFDFDEDDDFLSCVNGRGSIVNES
                     FDLPAFDAIVLDMSAEVIIQQGPQQEVTIEAQQNIIDRIRLTVRDREWIIKPDRCLRD
                     FSDVRIFITMPDVRRLELASSGRIFGENTFNIDDIELRLSGSGSIDLGLNCDDVTAAL
                     PGSGTIALEGTGDELDFNLSGSGKLKAFGMQMNEADISISGSGDAEVRVANRLSARIS
                     GSGNVLFRGNPVVNSTITGSGRVIDSN"
     gene            complement(6102..>6636)
                     /locus_tag="PK198_18440"
     CDS             complement(6102..>6636)
                     /locus_tag="PK198_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40780.1"
                     /translation="LYEFENAIGSQAADTIVGSTAGNQLAGGAGDDSIGGGGGLVLGN
                     VILGAYGLGAVDLKEVLQNDDIDRLELAHGGLWLGFTYPSYKAIHLYGGARLGWGGIN
                     IELDNPRVTDFDQIFVATPEMGIELNLTRWFRVAGTAGYRFVNGSAPGAAYTDKDFSG
                     PVAALTFRFGWFGNGRF"
BASE COUNT         1464 a         1777 c         1792 g         1478 t
ORIGIN      
        1 cggcagcagg atgtaggcgg cctgtacttt ggcggcgaac atcagcagga gcgcgggcac
       61 gatcagtcgt ttccagttca tcttttttca gttttttaag gaacggggcc gaaataaagt
      121 acacattttc agggagcaga tggggtgtct gaacgagcag aaaaacgtag ccgcagtggt
      181 gctgcggcga ggcttttatg tgaagtgcag gcggctcaga tgccgttgag gatgtgtagg
      241 ttattcggaa cccgttccta agacagaacc gcgacttaaa acactatggc agagcaaaaa
      301 gactcgacaa acaaacccgt ggccggcgca caacaacgac gacccggcgt gccaagaaaa
      361 ggcgaagacc tctcgaacta tgtgttcggg aaggtacagc ctcaggcttt gcctttggaa
      421 gaggccgtac tcggcgccgt catgctcgac aaaaatgccc tcaccgttat ccttgacatc
      481 ctgcagcccg acagcttcta cctcgaagcc aaccagcaca tctaccgcgc tatgcgccgc
      541 ctgttcgacc gttcgcaacc cattgatttg ctgacggtca tggaggaact caaaaaatcg
      601 ggcgacctgg aggcggtggg cggccctgct tacctcgccg aactcacgca ccgcgtagcc
      661 tctgccgcca acattgagta ccatgcgcgc atcatctccc aaaagtacat acagcgtgaa
      721 ctgattcggg tttctaccaa gaccatccgc gacgcctttg aagacacctc cgacgtgttt
      781 gaactcctgg acgaagcgga acagggcctg tacaacatcg cccaaaacaa tatgagtcgc
      841 ggctacgaaa gtacgggtac cctggccggc aaaatgctca aacaaataga ggaactcaaa
      901 ggtaaaaagg atggactcac cggcgttcct accggcttca ccgacctcga ccgcctcact
      961 tccggctggc aaccctccga cctcatcatt gtagcggcgc gccccggtat gggtaaaacg
     1021 ggcttcgtcc tttctatgac ccgcaatgca gccattgatt tcaaaaaacc ggtggcgctg
     1081 ttttctttgg aaatggccag cctgcaattg gtactccgtt tggtttctat ggaggccgag
     1141 atttcgggca gcaaactgcg caacggccag ctcgaagagg aggagtggaa acgcctgcac
     1201 aaatcggtgg aacgcatcag cgaagcgcct atttatatag acgataccgc cggcatcaac
     1261 atcttcgaac tcagggccaa agcccgccgt atgaagctcc agcacgacat ccagctcatc
     1321 atcattgact acctccagtt gatgagcggc ggccccgaca gcggtaaagg caaccgcgaa
     1381 caagagatca gctccatttc gcgctcgctc aaagccttgg ccaaagaact caatgtgccg
     1441 gtcattgccc tgtcgcagct cagtcgtgct gtggaaacac gcggcggcac caagcgcccg
     1501 cagttgtcgg acttgaggga atccggcgca atcgaacagg acgcagacat tgtctccttt
     1561 atctatcgtc ctgattatta ccaaattatg gaagatgaga atggccagag cctcaagggg
     1621 gtagctgaaa tcatcatagc caaacaccgc aacggcgcct tggagaccgt gaaactgagg
     1681 tttaccgaca agtttgcccg cttctccgat ttggacgagt tcaacttcga tgcactgccc
     1741 tccggcgttt ttcaagaccc caccccggcc aatgtcatca tccggccttc gcgcatgaac
     1801 gacgacgagg acattccgtt ttaatcatcc caatcccaat tttcgggaaa ctgctgccgg
     1861 tagtgttttt tggtaaatgc gcgctgccgc tgcctgtcca aaatcaactt ggccaatgcc
     1921 gtgtgcgccg gctcttcggc ggcgggagag agcgcgcgat ctaccttgcg ctcgtccaca
     1981 tccaaacggt acatcaggct catcagatac tccaagcggt gctcgatgag ccaggccacc
     2041 tgttcggaga gcagttccag caggcgctct tcggaaacgg cttcctgcaa aggcacaggg
     2101 tcgaaatcct gcgccaccaa tgctgtgagt tgttgcagat cgctgccttc ttcttccatg
     2161 ttggtactat ttcctgttgg ctctgatcca ggccagcact tcctgcgtag gttgggcagc
     2221 cacttcggcg ggcagagcca cgcgctcatt tttagccaga tcgaaggcgt aggcggtatt
     2281 ggcgcttgta ttttcgcgca gcacgatgta gcggttgttg gcggcgcttt tctggatgtc
     2341 ggttttgagc gcgatgggcg catccagcat cggattgacg gaaaaagtgc ccgcatctgc
     2401 attgtaactt ggcgaaatgg cctgaaaagc gccctgtacc ggcagcaaaa acagcgattg
     2461 cgcagcgccc gatgccggtt tgtattcggc caaggccagt accggcgcgg gtttggcttt
     2521 gtcggcgagg gtgaaagcaa aggcgccgaa atcctgcgca tagccgatga ggtagtcttc
     2581 ccaaagctct aagcgctgta tcatgcccga ctgcgcgtcg gcgccgctgc gggcggaggc
     2641 gaatttgggc ttgagttcgg gcagccagcg tcggttttcc gcatcgtaca ggtaaatgga
     2701 gttgaagccc cgaacggcag ccatttcatg tactttattg tagctaattt cggccacata
     2761 gtacggaaaa tcggggctga cattgacggg caacaccttg cgctccagca gactgcaatt
     2821 gcgcgtgtca tacacttcca atacgcggta gctgtcgcca tagtcggcgt caaacgtaga
     2881 ggaatcggcg gtgacggcca cgagaatttc ctgagaccga atccagatcg aagctccttc
     2941 gtcgagcatt ttgccggcta ctttgcaatc atgggtagcg cgcggcggcg gcggagtggt
     3001 cgctgtggtg tcggtagtcg tagtggcgcc ggagttgtcg ggtgctgggt tgccgcagtc
     3061 tgctgcgatg aatacagaca gggcaacaaa aaagtacttt gccgatttca ttgattcaaa
     3121 tttttcccga aggtacaaaa cctgctgcgt tgccatagct gcgtgacnnn nnnnnnnnnn
     3181 nnncgtgaca aaaatagcct tcgtttttcc tgaaaaattg ctaactttgt cctcatctgt
     3241 tgatttagct atcttaataa tcaccttcat aatattgggg taatgtcttc tgaaaaacgt
     3301 cgatacatag agcaacgccg cgggcttttt tggtctgtgg gcgacgaaaa actcgatcag
     3361 ataagcgacg aattgttggt ggaaaacatc ctgaactacg gcacgttgga agacgtgcgg
     3421 gagctgattt cattgatggg attgcaacag gaagcgctnn nnnnnnnnnn nnnnnnnnnn
     3481 naagaacaga cctcgaaaca actacttccc acaggtagcc aactttttcg atatctattt
     3541 ttcaaggcat gtacccgaat atcctctcta acggccaaaa ggaattgctg ccgattctgg
     3601 gtagcttgca acgcgagttt tacttggtgg gaggcactgc cattgctctg caaataggcc
     3661 accggcaatc tattgactac tccgaagaag ttaccttcct gcccagcttt gaggtgagca
     3721 atgacgaggt gaaacggttt ctaacagagg tagcgtcgag aagtatttag cacgattatt
     3781 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnncag gtggctttcc atcgaagcat caagggagga
     3901 agtgagcagg gaggcgtttt gttgttctac cgcgcgcacc cagtcgcggg ccagtcccca
     3961 gtcgccgccg ccatgtccgc tgctgatgtc ggcatggtcg cgtacgttcc atttcgtgat
     4021 ctcgccgttg cggaaatcag ctatgtagag ttcgttttcg tcgcccacca gatcgcccat
     4081 gctgcccatg atgcgggtgc ggcggcctgc ataattggtg aacgcctcca tgctgaagtt
     4141 gacggtgatc tgatcttcaa actccataga cacgatctga tggtcgggca cgttgttgtc
     4201 gcagcgatac acacagcggc catactgccc ctctttgagg tacttcataa tggcgtcgcc
     4261 ctgaagttcc ttttctgcgg gcagatcgaa atggtgcagc caggtgcggt ttttgtgata
     4321 aatgcgcaat gccgagtagg gacactgggc ctccacggcg catccgtcgg tacagcgggc
     4381 ggtgctgccg gcgggcgcat tttcactttt gaaccacttg agcgaaccga aagacgagat
     4441 gtagcggcag ttcttgccga gccaccagcg caggatgtcc aaatcgtggc aggatttggc
     4501 caagatgatg gggttggtat cttcctcgcg gcgccagttg ccccgcacat acgagtgcga
     4561 catgtgaaca tgctgtatcg gctccaagtg ctgcacgctg acgatgctcc ccagcacgcc
     4621 cgaatctatc acctctttca ccttgcggta gtacggcgag tagcgcaaca catggcacac
     4681 ggccacaatg cgcttgtatt ttttggcctg ttgcaggatg tcgttacatt ctttccaact
     4741 ttgcgcaatg ggcttttcca gcaatagatc gtagcccatt tcgagcgcct tcatggccgg
     4801 gccgtagtgc aggccatcag gcgtggtgat gatgacggcg tcggcaaatt tgggccgggt
     4861 gaagacgtgc tcccaggtca caaagcgatt ggcgtctatg atgttgtggt tttttgaata
     4921 gcgttcgttg cgcagcggaa taggctcggc cacgccgatg atgtccagct catcgggatg
     4981 tttcagggcg tagttgccat acacattgcc cctgctgccc gcgcccagcg taatggcggt
     5041 gatcttgcgg cctgctttgg gcagttgggt gtaatcccag gtgtagtcag cctcctgcat
     5101 cgggccggcc cacagttggt tgctcatggc tgcgcccaca gaaagagcgc ccatcgtttt
     5161 gagcaaatcg cgtcttgacc attcttgtgc catgttcttg ttgttttggt ggtaaagtta
     5221 gaaaaaccgc agagaatcgg cacaatacaa atacttcgtc catcggctac ttacggcttg
     5281 caaaaacgaa cctgcgacct ttacttcgcc acaggttcgt catgagttgc cggagaaacc
     5341 gctaattcga atcaatcaca cgccccgaac cggtgatggt agaattgact accggattgc
     5401 cccggaacag tacgttgccg ctgccgctga tgcgcgcact aaggcgattg gccactcgaa
     5461 cttctgcatc gccgctgccg ctgatagaaa tatcggcttc attcatttgc atgccaaagg
     5521 ctttgagttt gccggagccg ctgagattga agtccaactc atctcccgtt ccttccagtg
     5581 caatggtgcc ggagccgggc agggcagccg tcacatcgtc gcagttcagc cctaagtcaa
     5641 tggagccgga gccggaaagc cgcagttcga tgtcgtctat attgaaggtg ttttcaccaa
     5701 aaatacgacc ggaactggcc aattcgagcc gcctgacatc gggcatggtg ataaaaatgc
     5761 gcacatcgct gaaatcgcgg agacagcgat cgggcttgat gatccattcg cggtcgcgca
     5821 cggtgaggcg aatgcggtca atgatgtttt gctgcgcttc gatggtcact tcctgctgag
     5881 ggccttgctg aatgattacc tcggcactca tgtcaagtac aatggcatcg aaggcaggca
     5941 ggtcaaaact ttcgttgacg atgctccccc tgccgttgac gcaactgaga aaatcatcgt
     6001 cttcgtcgaa gtcgaaaaag caaccgggcg ccagtaccgt cacagccagt aagcccagta
     6061 tccatttgaa atttttcatg gtgatgttgt ttttgtttta attaaaatct cccgttgccg
     6121 aaccacccga agcggaaagt cagtgcggct accggccctg aaaaatcttt gtcggtatat
     6181 gccgcgccag gagcggagcc attgacaaag cgatagcctg ccgtgccggc cacgcgaaac
     6241 caacgggtga ggttgagttc gatgcccatt tcgggagtgg ccacgaatat ctgatcaaag
     6301 tcggtaaccc gcggattatc caattcgata ttgatgccgc cccatccgag ccttgcgccg
     6361 ccatacaggt gaatggcttt gtaagacgga taggtaaatc cgagccaaag gccgccgtgg
     6421 gccagttcga gccggtcaat gtcgtcgttt tgcagcacct ccttgaggtc aaccgcgccc
     6481 aggccgtacg cacccagaat gacattgccg agtactaagc cgccgccgcc gccgatgctg
     6541 tcgtcgcccg caccgccggc gagctggttg ccggccgtcg aaccgacgat cgtgtccgcc
     6601 gcctgtgagc cgatggcgtt ctcgaactcg tagagc
//
LOCUS       DAORLF010000274         6581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6306_length_6581_cov_2.032026, whole genome shotgun sequence.
ACCESSION   DAORLF010000274 DAORLF010000000
VERSION     DAORLF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6306_length_6581_cov_2.032026"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>279)
                     /locus_tag="PK198_18445"
     CDS             complement(<1..>279)
                     /locus_tag="PK198_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF40781.1"
                     /translation="LEQARKATGKSNLSMDDLRQEEDVVDTWFSSWLWPISVFDGFEN
                     QEELKYYYPTNLLVTGWDIMFFWVARMIMSGYEWAPELLGKDLIAQKGA"
     assembly_gap    280..341
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(342..1158)
                     /locus_tag="PK198_18450"
                     /pseudo
     CDS             complement(342..1158)
                     /locus_tag="PK198_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     assembly_gap    1159..1173
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1174..1604)
                     /locus_tag="PK198_18455"
     CDS             complement(<1174..1604)
                     /locus_tag="PK198_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF40782.1"
                     /translation="MELSTRYSPQETEAKWYEHWIRKGYFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDIMIRRARLEGKNACWVPGTDHASIATEAKVVKLLRERGIRK
                     GDISRDEFLQHAFDWKDKYGGIILDQLKRLGASCDWERTRFT"
     gene            complement(1681..2298)
                     /locus_tag="PK198_18460"
     CDS             complement(1681..2298)
                     /locus_tag="PK198_18460"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HRF40783.1"
                     /translation="MRIRPIIGALFVLFFTSCQSGYRKENGQWVWVSYDESVGKRITP
                     IDAHDAKSFRVLKSKKYAKDKNAVFYHGRLIEHADPRSFQVIHESGYSKDNNRVFLDD
                     HIIVLADPATFEFLEFPYTKDSDHVFCGNIPLKISPQEVNEFKVTNTDKLMSATKSTI
                     LFSHFLEMNPDYQWLDTTGITGIVVGDWGTGATKNKKFKGFREAN"
     gene            complement(2441..2854)
                     /locus_tag="PK198_18465"
     CDS             complement(2441..2854)
                     /locus_tag="PK198_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRF40784.1"
                     /translation="MYPAELTAPMAQDLTNYGFESLHSAEEVTTALNNTEGTALVVVN
                     SVCGCAAANARPGAKLAARHDKRPDRLLTVFAGVDQAATAKAREFMLPYPPSSPSIAL
                     FKDGKLVHFLERMHIEGRSAEIIAENLKMAFERYC"
     gene            complement(3017..3820)
                     /locus_tag="PK198_18470"
     CDS             complement(3017..3820)
                     /locus_tag="PK198_18470"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF40785.1"
                     /translation="MSIFKDTERPNIIHLRNISQSYDGGKTFIIKDLEFLVEDKPAQG
                     QFVVILGASGCGKSTLLRYIADLQKPTSGEVLLHDKPLDASKRVSMVFQQYSSLPWMT
                     VLDNVALPLSFQNVPEKERRDKAMEMIQLMGLEGHENKYAQYPTLSGGQLQRVAIARS
                     LMANPEILLMDEPFGALDINTRLQMQDLLAKIWLKFHPTVVFITHDIAEAVYLGDDIY
                     MMKKAPSRIVEHVQVDLPFERNKDTKRLPHFIELVQEVEDKLIRVTEMQ"
     gene            complement(3836..4957)
                     /locus_tag="PK198_18475"
     CDS             complement(3836..4957)
                     /locus_tag="PK198_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF40786.1"
                     /translation="MSLFTLRGGLSRPQSIALGIAGLILFFLAWWGLAEFYSEQKPII
                     EGYETRLPSAFADDDSLAVHIDLDSLARADSAAFANAKVFEKVYPLLPPPQRVFTSYP
                     GLIQKDELLKNTWRSVWLNIQGYFWAVLISIPLGFLIGLLPLFRGLFSKQVDALRYLP
                     LTALTGVFIIWFGIEDQMKIAFLAFGIIVYLLPVVVQRIDEVQDVYLQTVYTLGAKNW
                     QTIKSVYIPAVMSKLMDDIRVLTAISWTYIIIAELLNRKGGIGSLIYLKARQGKLEGM
                     FAALIVIILIGFLQDRIFSYFDKRLFPHKHLKSVREGLKETEYGILALLGTVLLLILQ
                     SEFVPSLSGIFATLAPVLIVTSIIIILYGEFKLFQSKTQ"
     gene            5029..5868
                     /locus_tag="PK198_18480"
     CDS             5029..5868
                     /locus_tag="PK198_18480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF40787.1"
                     /translation="MKFSEILPALRRRVRFFWEAQTRYDIHSPFVAGLVEAVLEDNRN
                     YYAFSDIEVARSRWLADEEMIHLPRLGAGSTISDASQRRAGDILRSTAVGPETGRRLF
                     RLALWLQPESILELGASMGISTAYLAAADARIPLLSIEGNAAVATKARQHLADLGLQH
                     VELVSGLFEEQLPAALSKLGRLGLLFIDGDHRKEAVLKHVQTCLQHHHNDSVFVIADI
                     HWSEDMELAWSELCALPEVTLSVDLFHFGLLFFKKELLEKQHFVVVPAHWKPWRMGFF
                     SGR"
     gene            complement(5932..>6215)
                     /locus_tag="PK198_18485"
     CDS             complement(5932..>6215)
                     /locus_tag="PK198_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40788.1"
                     /translation="IPNAFSPNNDGTNDRLMVFAGEDVAYIRSFQVFNRWGESVFEVY
                     NFPPNDPAYGWDGNHRAQLYNAAVFAYFAEVEFIDGSVKLFKGDVTLIK"
     assembly_gap    6216..6226
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6227..>6581)
                     /locus_tag="PK198_18490"
     CDS             complement(<6227..>6581)
                     /locus_tag="PK198_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40789.1"
                     /translation="FYPNLGGGTYSIVVQDATGCELAMEVSLPQGNDLVLDLGPDQFI
                     RLGQRANVQAQYNIPENEVASFQWGATDSLPCNGCLQFEVRPFNTVIYSATLVDNNGC
                     RITDDVTIFVANPREV"
BASE COUNT         1478 a         1617 c         1752 g         1646 t
ORIGIN      
        1 gcgccttttt gagcgatcag gtctttgccc aacagttcgg gcgcccattc gtagcccgac
       61 ataatcatgc gcgccaccca gaaaaacatg atgtcccagc cggtgacgag cagattggtg
      121 gggtagtaat atttcagttc ttcctgattt tcaaagccgt cgaacacgga aatgggccac
      181 aaccaggagg agaaccaggt gtccaccaca tcttcttcct ggcgcaggtc gtccatgctc
      241 aggttggatt tgccggtggc tttgcgggcc tgttcgaggn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccgttgg cttcatagcg
      361 gaggtaccag gcgggaatgc gctgtcccca ccagagttgc cgggagacgc accagtcgcg
      421 cacgttttcc tcgcgcagcc attggtagta catattccac atgccttccg ggtaaaattt
      481 cacctcgcct ttgcgcacgg cctccagcgc agaggcggcc aggtctttca tttccaaaaa
      541 ccactgcaaa gacaggcgcg gctccactac ggcgtcggtg cgttcggagt ggccgatttt
      601 ggtgcgatag tcttccaatt tgaccaagtt tccgctctcc tccagcagcc ccttgatctt
      661 tttgcgggca gcgaaacggt cttcgcccac caagatgatg gccgcttcgt tgagcttgcc
      721 gtcttcgtcg aggatgtcta tgacttccag tccgtggcgc ttgccgattt cgaagtcgtt
      781 ggggtcgtgc gccggagtga ttttgagggc gccggtgccg aattcccggt ccacataagt
      841 atcggctatg atgggaatag ccttgtcaat cagcgggata agtacctgtt tgccgatcca
      901 tgcctgcgct cgtcttccgg gtgtacggcc accgccacgt cggccatgat ggtttcggga
      961 cgttgggtag cgatgacgat gccctgtgtg gggtcgtctg ccagcagata ttggatgtga
     1021 aacaggcggg cattttcctc ctgataaagg acttcctcgt tggagagtac cgttttggcc
     1081 tccgggtccc agttggtcat gcgtttgccg cgatagatgc ggcctttgcg gtacaagtcc
     1141 acaaaaacct tgatgacgnn nnnnnnnnnn nnngtgaagc gggtgcgttc ccagtcgcag
     1201 gaagcgccga ggcgtttgag ctgatcgagg atgatgccgc catatttgtc tttccagtcg
     1261 aaagcatgtt gcagaaactc gtcgcgggaa atatcgcctt tgcgaatgcc gcgctcgcgc
     1321 agcagtttca ccaccttggc ctcggtagcg atggaggcgt ggtcggtgcc gggtacccaa
     1381 caggcgtttt tgccttccaa cctcgccctg cgaatcataa tatcctgaat ggtgttgttg
     1441 agcatgtgcc ccatgtgcag cacgccggtg acgttgggcg gcggaatgac gatggtatag
     1501 ggttcgcgct catcgggcgt ggagtggaaa tagcctttac gaatccagtg ttcgtaccat
     1561 ttggcttcgg tttcctgcgg actgtatcga gtagagagtt ccatcttcgc ggttgcttgt
     1621 tgttaagggc cgcaaagata ggggattttg gggaaggagt tgtaagcgtt ttagcggtgc
     1681 tcaatttgcc tctctgaacc ctttgaattt tttgttcttc gttgcccccg taccccaatc
     1741 tcctaccaca attccggtaa ttcccgtcgt gtcgagccac tggtaatctg gattcatttc
     1801 gagaaaatgg gagaacagaa tggtggattt tgttgcggac atcagtttgt cggtgttggt
     1861 tactttaaat tcgtttactt cttgtggact gattttcaag gggatatttc cgcaaaaaac
     1921 gtgatcgctg tcttttgtgt atggaaattc caggaactca aaggtagccg gatcggccag
     1981 gactatgata tgatcatcaa gaaaaacgcg gttgttatct tttgaataac cagattcatg
     2041 aatcacctga aagctgcgcg gatcggcatg ttcgatgagc cggccgtgat agaacaccgc
     2101 atttttatcc ttcgcgtatt ttttactctt cagaaccctg aaacttttcg cgtcatgcgc
     2161 atctatcgga gtaattcttt tgcccaccga ctcatcataa gatacccaaa cccattgacc
     2221 gttctctttt cgatatcctg attgacaaga tgtgaaaaac aaaacaaaca gcgctccgat
     2281 gattggcctg attctcattt ttatatttgg tttacaaggt gttgtatttc gttggatgtc
     2341 cgttgattag catcttgttg tgagttgtaa aacgaaaaac agcctgtgac gggagtgctg
     2401 tcgcaggctg tttgttcagt tgtcatcaag cgttggctgc tcagcaatac cgttcaaaag
     2461 ccatcttcag gttttcggcg atgatctctg cggagcggcc ttcaatgtgc attctttcga
     2521 ggaagtgtac caatttgccg tctttgaaca aagcgatgga cggcgaagag ggcgggtaag
     2581 gcaacataaa ctctctggcc ttggcagttg cggcctggtc aacgccggcg aacaccgtca
     2641 acaggcggtc ggggcgcttg tcgtggcggg ccgctaattt ggcgcccgga cgagcattgg
     2701 cggcagcgca gccgcatacg gaattgacga cgaccaaagc cgtgccttca gtattgttca
     2761 atgctgtcgt tacttcctca gcactgtgca agctctcgaa accatagttg gtgaggtctt
     2821 gagccatggg tgcggtaagt tctgctggat acatggatat tatcgttttg gtagttgttt
     2881 gaataaaatc ggctataaat acagcggaag gcggcacttg gttcattcat gtgggcagga
     2941 tagtgaaggg gtgactatcc gttcaggcag agaaagtcac cccttcacta tacgctgata
     3001 ttcagcaatc acttcattac tgcatctccg tcacccgaat cagcttgtct tccacttcct
     3061 gcaccaattc gatgaaatgc ggcaggcgtt tggtgtcttt gttgcgttca aacggcaaat
     3121 ccacctgtac atgctctacg atgcgggagg gcgctttttt catcatatag atgtcgtcgc
     3181 cgaggtaaac ggcttctgca atatcgtgtg tgatgaaaac gacggtgggg tgaaatttga
     3241 gccagatttt ggccagcaag tcctgcattt gcaggcgggt gttgatgtcc aatgcgccga
     3301 atggttcgtc catgagcagt atttcagggt tggccatcag gctccgggca atggctacgc
     3361 gctgcaactg cccgccggag agggtagggt actgggcata cttgttttcg tgtccctcca
     3421 aacccatgag ctgaatcatt tccatcgcct tgtcgcggcg ttccttttcg ggtacatttt
     3481 ggaaactcag tggcagcgct acgttgtcca aaaccgtcat ccagggcaaa gatgaatatt
     3541 gctgaaagac catgctcacg cgtttggacg cgtccaatgg cttgtcgtgc aggagcactt
     3601 cgccgcttgt gggcttttgg aggtcggcga tgtagcgcaa gagggtggac ttgccgcaac
     3661 ccgatgcgcc gaggatgacc acaaactggc cttgagcggg tttgtcttct accaaaaatt
     3721 ccaggtcttt gataatgaag gttttacctc cgtcgtaact ctggctgata ttgcgcaggt
     3781 ggatgatgtt gggacgctct gtatctttga aaatgctcat gattgggcag ttgttttatt
     3841 gagttttgct ttgaaagagc ttgaactcgc cgtaaagaat gatgatgatg gaagtgacga
     3901 tgagcaccgg cgccagcgta gcgaaaatgc ccgacaagga gggtacaaat tcactttgca
     3961 ggatcagcaa caaaaccgtt cccaacaaag ccagaatgcc gtattcggtt tctttcaaac
     4021 cctcgcgcac cgatttgagg tgtttgtgcg gaaagaggcg cttatcgaaa tagctgaaaa
     4081 tgcgatcttg caagaaaccg atcaggatga tgacgatgag cgccgcaaac atgccttcga
     4141 gcttgccctg gcgcgctttg aggtatatga gcgagccgat gccgcccttt cggttgagca
     4201 actccgcaat gatgatgtac gtccacgaaa tggccgtcag cacccgaatg tcgtccatca
     4261 gtttggacat cacagcagga atatagacgc ttttgatggt ttgccaattc ttggcgccca
     4321 gggtgtaaac ggtttggaga tacacatcct gcacctcgtc tatgcgctgc acgactaccg
     4381 gcaacaaata gacgatgata ccaaaagcga ggaacgctat tttcatctgg tcttcgatgc
     4441 cgaaccaaat aatgaaaacg cccgtgagcg cagtcagggg caggtatcgc agggcgtcca
     4501 cctgtttgct gaaaagcccg cgaaacaaag gcaacagccc aatgaggaag cccaagggaa
     4561 tagaaatgag cacagcccag aaatagcctt gaatattgag ccagaccgag cgccaggtgt
     4621 ttttcagcaa ctcgtctttt tgaatcaggc cgggataaga agtaaatacg cgctgtggcg
     4681 gcggcagaag cggatatact ttttcaaaaa ccttggcatt ggcgaaagcc gcagaatcgg
     4741 cacgggccag cgagtctaaa tctatatgca cagccaatga gtcgtcatcg gcaaaggcag
     4801 aaggcaggcg ggtttcgtag ccctcgatga tgggcttttg ttctgaataa aattctgcca
     4861 ggccccacca ggccaagaag aaaagaatca gcccggcaat accgagcgca atgctttgtg
     4921 gacggcttaa cccgccccga agtgtgaaca gcgacatatt ttgatataaa ttagcgcccg
     4981 aaattatgct tttcttccat ttgatctatc atttttatcc tcctgcgcat gaaattttct
     5041 gaaatactgc cggcattgcg gcgtcgcgta cgcttctttt gggaggcaca aacgcgttac
     5101 gacatccatt cgccttttgt ggccggatta gtagaggctg tgctggaaga caatcgcaat
     5161 tattatgcgt tttcagatat tgaagtagcc cgcagcaggt ggctggccga tgaagaaatg
     5221 atacacttgc caagacttgg cgcgggttct accatcagcg acgcctcgca acgcagggcc
     5281 ggagacatcc ttcgcagcac cgccgtcggt cccgaaaccg ggcgcaggtt gtttcgcctg
     5341 gcgctatggc tacagcccga atccatcctc gaattgggtg cctccatggg catttccacc
     5401 gcgtatctgg ctgccgccga tgctcgaatc cctctgttga gcatcgaggg caatgcggct
     5461 gttgcgacga aagcgaggca gcatcttgcc gacttgggtt tgcagcatgt agagcttgtt
     5521 tccggtctgt ttgaggagca attgcccgcc gcgttgagca agttgggccg cttgggtttg
     5581 ctctttattg acggcgatca ccgcaaagaa gccgttttga aacacgttca aacctgtttg
     5641 caacatcatc acaacgacag cgtattcgtc attgccgaca ttcactggtc ggaggatatg
     5701 gagctggcct ggagcgagtt atgcgccctg ccggaagtga ccctttcggt tgatctgttt
     5761 cacttcgggt tgttgttttt taaaaaagaa ctacttgaaa aacagcactt tgtcgttgtg
     5821 cctgctcatt ggaaaccctg gcggatgggc tttttttcgg gtagataata catacaaagg
     5881 ggcgactccg ccaagacaga gccgcccctt cgcacatctg gtttaaacag attattttat
     5941 cagcgtcaca tctcctttga acaacttcac gctgccgtct ataaactcca cctctgcaaa
     6001 ataagcaaag acagcagcat tgtaaagttg agcgcggtga ttgccgtccc aaccatacgc
     6061 cgggtcattg ggcgggaaat tatacacctc aaaaacagac tctccccagc gattgaacac
     6121 ctggaaagaa cgtatgtatg ccacgtcctc gccggcaaag accatcagac ggtcgttggt
     6181 gccgtcgttg ttgggcgaga aggcattggg aatgannnnn nnnnnncact tcacgcggat
     6241 tggccacaaa aatggtcacg tcgtctgtaa ttcggcagcc gttgttgtcc accaatgtag
     6301 cggagtaaat gacggtattg aaaggccgta cctcaaattg caaacaacca ttgcagggca
     6361 gggaatccgt agcgccccat tggaacgacg ccacttcatt ttcaggaata ttgtactgcg
     6421 cctgcacatt ggctctttgg cccaatcgaa taaactgatc cggccccaaa tccaaaacaa
     6481 ggtcattgcc ctgcggcaag ctgacttcca tagctagttc gcagcccgta gcatcctgaa
     6541 cgacaatgct gtacgttccg ccgccgaggt tggggtagaa g
//
LOCUS       DAORLF010000275         6543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6350_length_6543_cov_1.769112, whole genome shotgun sequence.
ACCESSION   DAORLF010000275 DAORLF010000000
VERSION     DAORLF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6350_length_6543_cov_1.769112"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>483)
                     /locus_tag="PK198_18495"
     CDS             complement(<1..>483)
                     /locus_tag="PK198_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40790.1"
                     /translation="TVVVTRRRPLLAMKFRYEPNGNIRELQWKVTRQDVKYYENDYDP
                     INRLLGSNYGIEREVQPAIGAAYIERLNTQDYNEFGIEYDPIGNIKKLSRRGLIPAAD
                     CFTPGLIDNLSYSYGDEGRLTAVSDAALDPYRQEGFKPAAGGGPQATHYLYDHNGNLT
                     D"
     assembly_gap    484..629
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<630..>997)
                     /locus_tag="PK198_18500"
     CDS             complement(<630..>997)
                     /locus_tag="PK198_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40791.1"
                     /translation="TALYRVRMDNSAAPVWRFGGELVTAGQNYRRELRIPVQNAQERF
                     SILMTGDSIPVVWTLAQLLTQRQQNGLSGVASIERVIEKTNTNPCTLPPLNCSEKQTE
                     LQNAALAQIQNLLCSTNAAQL"
     assembly_gap    998..1016
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1017..1788)
                     /locus_tag="PK198_18505"
     CDS             complement(<1017..1788)
                     /locus_tag="PK198_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40792.1"
                     /translation="MNWNDRDQMTSKKLGYAGNLSLDLIGYRYNERGWLTDINDVIRE
                     RWETPYAICQGEAGEEETSQQEEKVPLSELLQMLCRGESVAVQGADPCAQGDCTDELA
                     DRIIEIRHEVALGGPRPTVTLSLHGLHIGGTPIGLPRLPYRWDFGPAGLTQNDSLALL
                     SDLSVWLQTNGYRFDSLQLRLLPPDWNQKSSTVGLRLSILQTNLSLSHAFYSINNQTP
                     YSRVTVRKENIRFVACRAIPTPEQEPRIIGLAQSTSQLQ"
     gene            complement(1785..>2350)
                     /locus_tag="PK198_18510"
     CDS             complement(1785..>2350)
                     /locus_tag="PK198_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40793.1"
                     /translation="PGARAQILRYYDENNLLKYTIDGKGQRIDHYYDEYLRERRTTLA
                     TVTNWDPQSSSASHGAPGSDILITRYGEEFPDLPQIYKGRVRQTEAKLFGDGPVTASG
                     FTQTTFSYDNFGRAINRSETFPLLPGSGTGTDTWELLFDHADRLKFEGRSHAGAASVS
                     TRMNTTTSGASFTTPSAQASPPAQRWP"
     assembly_gap    2351..2407
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2408..2617)
                     /locus_tag="PK198_18515"
     CDS             complement(<2408..2617)
                     /locus_tag="PK198_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40794.1"
                     /translation="MELLPDGATRKHKFTVSENCPGTAVTDEKGNTSQMYQDFLGRQS
                     KIKDALNHNTTFKYFKHGGGKTVQPP"
     assembly_gap    2715..2733
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2734..3742)
                     /locus_tag="PK198_18520"
     CDS             complement(<2734..3742)
                     /locus_tag="PK198_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40795.1"
                     /translation="MMNAWLLTAQVTSSGIQLPPSPEASGMGKYGDIPVGEHAGTANV
                     SVPIYEIQTRQGAVPISLNYHTGGVRVAQEAEWTGLGWSLQAGGSITRAIRGLDDFGD
                     HVGHCVNAPFENYYQRGIGNNASGSPRGNLINPGYYEYASDLGADFTCMVDRHITGIP
                     VRITAPDGVKATTQYNCTYLVPSLFRETYTDGTSIVQSQVLQFNTQGYPVSLQIFGFP
                     SNESYVWNNGLLESKSYLNWQHHFDYYTNRTLKSSQGIDGKKAEFIYDPYQRLYQIKA
                     RPFEPGSTNFHIQTEITYQMGGNNVVRSKTTYSDAPSRIVEKHFDGLGRPVREILNGV
                     LK"
     assembly_gap    4286..4311
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4312..>4655)
                     /locus_tag="PK198_18525"
     CDS             complement(<4312..>4655)
                     /locus_tag="PK198_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40796.1"
                     /translation="SATLRIEKVSTGYKQVSVELAGFAGPVSLMLKSEKQEILVNESV
                     PVGAAFAKIFNLSQLPAGRYELHVGSETKDVVQPLTVTEADVILTEGQRREFFAPTAL
                     LRGRNLDFNWFS"
     assembly_gap    4656..4732
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4868..5167
                     /locus_tag="PK198_18530"
     CDS             4868..5167
                     /locus_tag="PK198_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF40797.1"
                     /translation="MKNYRWGILGIGKIAEKFAADLALLPQARLTAAASRSEDRAADF
                     ARRHDAPLHFGSYEALAACPDVDIIYIATPHVAHCEAAMMCLEQGKAVLAKSHWP"
     gene            5164..5832
                     /locus_tag="PK198_18535"
     CDS             5164..5832
                     /locus_tag="PK198_18535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF40798.1"
                     /translation="MNASQVERMMDASARHNAFLMEAVWTRFMPPTLTMLELIQSNAI
                     GQVTAVKADFGFAARYDPEGRLFNPAFGGGALLDIGIYPAFLALLVLGIPARIRAASA
                     FGPSGVDEDTGMMFEYDNGALAHLHANIRYDTPIEAQIYGTTGHIHLHSRWHHARELT
                     LHRKGSTPEVFRFDYPGLGYQFEAEEVMRCLDAGLRESPLLPLHFSLSLAKLLDQTAL
                     ETVR"
     gene            5934..>6543
                     /locus_tag="PK198_18540"
     CDS             5934..>6543
                     /locus_tag="PK198_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40799.1"
                     /translation="MMKNLLLFALLISTAPFLVAQKVALTFTPAVVNVDDVIDMNDPA
                     FEMVGYATVTNTSNTPISVRWNRVAPQLPMGWEVLVCDNESCYPPFVYSNVIPEFDLN
                     MPVVLQPGGTTNMDVHVKPNQLPGSTVVTIQLTAANDTTVIATGTYNFGASITSSSSQ
                     PWGARQDIRVFPNPTTDYFQLSPGSKVSRVTVYNTLGRQVRTF"
BASE COUNT         1269 a         1638 c         1748 g         1544 t
ORIGIN      
        1 gtcggtcagg ttgccgttgt ggtcgtacag gtagtgtgtt gcctgtggcc cgccgcctgc
       61 ggcaggcttg aagccctcct gccggtaggg atcgagtgct gcgtcgctca ccgccgtcag
      121 tcggccttcg tcgccgtagg agtagctcag attgtctatc agtcccggcg tgaagcagtc
      181 ggcggcgggt atcagtcccc ggcggctcag ctttttgatg ttgccgatgg ggtcgtattc
      241 tataccaaac tcgttgtagt cctgcgtgtt caggcgctct atgtaggccg cgcctatggc
      301 gggctgcacc tcgcgctcta tgccgtagtt gctgcccagc aggcggttga tggggtcgta
      361 gtcgttttcg tagtatttta catcctggcg ggtcactttc cattgcagtt cgcggatgtt
      421 gccgttgggt tcgtagcgga acttcatggc caacaggggc cggcgccggg tgacgactac
      481 cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gttgtgcggc gttggtactg cacagcaggt
      661 tttgtatctg cgccagggcg gcgttttgca gttctgtctg tttttcactg caattgagcg
      721 gcggcagggt gcagggattg gtgttggttt tttctatcac ccgctctatg gaggccacgc
      781 ccgacaggcc gttttgctgc cgttgggtca gcaattgggc cagtgtccac accaccggaa
      841 tgctgtcgcc ggtcatcagg atgctgaatc gctcctgtgc gttttgcacc ggaatgcgca
      901 gttcgcggcg gtagttttgc ccggcggtca cgagttcgcc tccgaagcgc cacacgggtg
      961 ccgcgctgtt gtccatgcgc accctgtaca gcgccgtnnn nnnnnnnnnn nnnnnnactg
     1021 caactgcgag gtgctctgcg ccaggccgat gatgcggggt tcctgttcgg gggtgggaat
     1081 ggcccggcag gctacaaagc gtatgttttc cttgcggacc gtcacgcggc tgtagggcgt
     1141 ctggttgttg atgctgtaaa aggcgtggct cagcgagagg ttggtttgca ggatgctcag
     1201 gcgcagaccc acagtggaag atttttggtt ccagtcgggc ggcagcaggc gcagttgcag
     1261 gctgtcgaag cggtagccgt tggtttgcag ccagacgctc aggtcgctca gcagggccag
     1321 actgtcgttt tgggtcaagc ccgccggccc gaagtcccag cgatagggca gtcggggcag
     1381 cccgatgggc gtaccgccga tgtgcagacc gtgcagactg agggtcacgg tggggcgcgg
     1441 cccgccgagg gctacctcgt gccggatttc gatgatgcgg tcggccaatt catccgtgca
     1501 gtcgccctgc gcgcaggggt cggcgccttg tacggccaca ctttcgcccc ggcacagcat
     1561 ttggagcagt tcgctcaggg gcactttttc ttcctgctgg gaggtttcct cctcgccggc
     1621 ctcgccctgg cagatggcgt agggcgtttc ccagcgctcg cggatgacgt cgttgatgtc
     1681 ggtgagccag ccgcgttcgt tgtagcggta gccgatgagg tcgagcgaga ggttgccggc
     1741 atagccgagt tttttggaag tcatctggtc gcggtcgttc cagttcatgg ccaccgctgc
     1801 gccggagggc tggcctgcgc cgaaggagta gtaaagctcg cgcccgaagt ggtcgtattc
     1861 atgcgagtgg aaacggaggc cgcgccggcg tggctgcggc cctcgaattt cagccggtcg
     1921 gcatggtcga acagcagttc ccaggtgtcg gtacccgtgc cgctgccggg cagcagggga
     1981 aaggtctcgc tgcggttgat ggcgcgcccg aaattgtcgt aactgaaggt ggtctgcgta
     2041 aagccggaag ccgtcaccgg gccgtcgcca aagagtttgg cttcggtttg gcgcacccgg
     2101 cctttgtaaa tctgcggcag atcggggaac tcctctccat agcgggtgat gaggatgtcg
     2161 ctgccgggcg ctccgtgcga ggcgctgctg ctttgggggt cccagttggt cactgtggcc
     2221 aaggtggtgc ggcgctcgcg caggtactcg tcgtagtagt ggtctatgcg ctgccccttg
     2281 ccgtcaatgg tgtatttcag caggttgttc tcgtcgtagt agcggagtat ctgcgcccgc
     2341 gcgccgggta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnggg aggttgtacc gtttttcccc cgccatgttt aaaatatttg aatgtcgtat
     2461 tatggttcag tgcatccttt atcttgcttt ggcgtcccag aaaatcctga tacatctgcg
     2521 atgtatttcc tttttcgtcc gtcactgctg ttcccgggca attttccgat actgtaaatt
     2581 tgtgtttgcg ggtggcgcca tcgggcagta attccaatat cattcgacca agcggcgagc
     2641 cgtcgtattc aaatgcgaca taattgcccg gcaacagggt ttgccgttgt accctgtctg
     2701 attggtcgta aacgnnnnnn nnnnnnnnnn nnnttttcaa aacaccattg agtatttccc
     2761 tgacaggacg ccctaaacca tcaaaatgtt tttctacaat ccggctcgga gcatccgaat
     2821 aagtagtttt tgacctgacc acattgttac cgcccatctg gtaggtaatt tccgtctgta
     2881 tgtggaagtt cgttgatccc ggctcgaaag gtctggcttt gatttggtaa agccgctggt
     2941 aaggatcata aataaactca gccttcttcc catctatgcc ctgcgagctt ttcagtgttc
     3001 tgttggtgta ataatcgaaa tgatgttgcc agttcaggta agattttgac tccaacaagc
     3061 cgttattcca aacatacgat tcattggatg gaaagccaaa tatctgcaag gatacaggat
     3121 agccctgcgt attgaactgt aaaacctgac tttgaacaat ggaggttccg tctgtatagg
     3181 tctctcgaaa caaggagggt accaaataag tacagttgta ttgtgtggtg gcttttactc
     3241 cgtcgggagc tgtaatgcgc acggggatgc cggtgatatg acgatctacc atacaggtaa
     3301 agtcggcgcc aagatcgctc gcatactcat aatagccggg atttatcaaa tttcccctgg
     3361 ggctgcccga cgcattgttg ccgatgcctc gctgataata attttcgaat ggcgcattga
     3421 cacaatgccc gacatgatct ccgaaatcgt ccaaacctct gatagcgcgc gttatggaac
     3481 cgccggcctg aagcgaccaa cctaagcccg tccattctgc ttcttgtgct acccgcacgc
     3541 cgcctgtgtg gtaattcaac gatatgggta cagccccttg tcgcgtttga atctcataaa
     3601 tcggaacgct tacattagct gttcctgcgt gttccccaac gggaatatcg ccgtatttgc
     3661 ccatgccgga agcttccggt gatggtggca attgtatgcc ggacgaggtc acttgtgccg
     3721 tcaacaacca tgcattcatc atcaataggg ctgaaaagat aaatctatgg aatggtaata
     3781 acagggccgt atatgcctga tactgagtta aaaaatttaa agcactccaa acaggggggg
     3841 gggcaaaata atcttctgca tgaggttaaa tattttcggg ttaaagaact ggtgcaaaaa
     3901 taggggcgaa tttttaaaaa gtcaagaggg cgagggaggt tttttgggag tttaacattt
     3961 gggggggcag cagcgagaca ctccttttta cacgacgcct ctaatccgcc agatttaata
     4021 cgaagtgtct gttttccccc caaaaaacag cccgcaggct attcccgcgg gccgtgtaaa
     4081 tgaaacctaa tgtatgatgt ctgcaaaaaa aactcagttc agcgcgatgg actggtagta
     4141 cgttttgtgc ggagtagtga atcgcagcgt gtattgcccg cgcggcaggt gttcgaggcg
     4201 atagcggcgc tctacccgaa agacgttttt gacggcgtcc ttaaaaaatg gaactccgtt
     4261 atcatccaaa atgacgactt ccactnnnnn nnnnnnnnnn nnnnnnnnnn ngctgaacca
     4321 gttgaagtcc agattgcggc cgcgcaggag tgcggtaggt gcaaaaaact cgcggcgctg
     4381 cccctctgtc aggatcacat cggcctccgt gaccgtcaaa ggctgtacta catctttggt
     4441 ctcagaaccg acgtgcaact cgtaacggcc ggccggcaat tgagacaggt tgaatatttt
     4501 ggcgaaagcc gcgccgaccg gtaccgactc attcactaag atttcttgtt tttccgactt
     4561 cagcatcagt gatacaggtc cggcaaatcc ggccagttct accgacacct gcttatagcc
     4621 ggtgcttacc ttttcgatgc gcaaagtggc ggacgnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatttctca
     4741 aaatcttctt cattttcaat ggtttaatga aacaattggg tgatttttac gccgcaaagt
     4801 taccggcaca aagaagccgg gcgctactgc ttttttaaca gcttttgcgc tcaaattgac
     4861 cacaaccatg aaaaattacc gctggggcat actcggaatt ggaaaaatag ccgaaaaatt
     4921 tgccgccgat ctcgctctcc tgccccaggc ccgactaacg gctgccgctt cccgctcgga
     4981 agatcgcgct gccgatttcg cccgcaggca cgacgccccc ctgcattttg gttcttacga
     5041 agccttagct gcttgtcccg atgtggacat tatatatatt gctacgccac acgttgctca
     5101 ttgcgaagct gccatgatgt gtctggagca gggcaaggcc gtgcttgcga aaagccattg
     5161 gccatgaatg cttcgcaggt ggaacgtatg atggacgcgt cggcccggca caatgctttt
     5221 ttgatggaag cagtgtggac gcgattcatg ccgcctaccc tgacgatgct ggaactcatt
     5281 caatccaatg ccatagggca agtgacggca gtaaaggccg acttcggttt tgctgcccgc
     5341 tatgaccccg aagggaggct gttcaacccc gcgttcggcg gcggcgccct gctcgacatc
     5401 ggcatttatc ctgcattttt ggcattgctg gtgttgggaa tacccgcacg catacgggcg
     5461 gcttctgcat tcggccccag cggcgtggat gaggatacag gaatgatgtt tgaatacgat
     5521 aatggagcat tggcccatct gcatgccaac atccgctacg acacgccgat agaggcgcag
     5581 atttatggaa ctaccggcca tattcatctg cacagccgct ggcaccatgc ccgcgaactg
     5641 actttgcacc ggaaaggcag tacgcccgaa gtgtttcggt tcgattaccc cggcctgggg
     5701 tatcagtttg aggcggagga agtgatgcgg tgtttagacg ccgggctgcg ggaaagcccg
     5761 cttttgccgc tccatttcag cctgtctttg gcaaaacttc tggatcaaac agcattggaa
     5821 actgtgcgat aacctgtttt gtcgcttatt tcacatttca ttatgaaacc agggcttgat
     5881 tcttagagat attaatcgga actttgcagc gacgcctaaa tgtcaaaaac gaaatgatga
     5941 aaaatttact actgtttgca ctgctgattt ccactgcgcc attcctcgtt gcgcaaaaag
     6001 tggccctcac atttacacct gctgtcgtta acgtggacga tgtgattgat atgaacgacc
     6061 ccgccttcga gatggtaggc tatgccactg ttaccaatac ctccaatacg cctatctccg
     6121 ttcgctggaa tcgagtggcg cctcagcttc cgatgggttg ggaagtgctg gtttgcgaca
     6181 acgaatcctg ctaccctcca tttgtctatt ccaatgtcat tcccgaattt gacctcaata
     6241 tgcctgtcgt gctccagccc ggcggcacta ccaatatgga cgtccatgtg aagcccaacc
     6301 aattgcccgg cagcaccgtt gtgaccatcc aactcaccgc agccaacgat acgacggtca
     6361 tcgctaccgg cacatataat ttcggcgcca gcattaccag cagcagttca cagccctggg
     6421 gcgcgcgtca ggacatccgc gtatttccta atcctaccac cgactacttc caactctcgc
     6481 cgggcagcaa agtgagccgc gtaacggttt acaatacttt gggtcgtcag gtacgcactt
     6541 ttg
//
LOCUS       DAORLF010000276         6528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6375_length_6528_cov_1.906689, whole genome shotgun sequence.
ACCESSION   DAORLF010000276 DAORLF010000000
VERSION     DAORLF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_6528_cov_1.906689"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PK198_18545"
     CDS             complement(<1..1195)
                     /locus_tag="PK198_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF40800.1"
                     /translation="MQINKIILFASFLLGTAFALNAQTESGFAPSGEVQIHYKTYGTG
                     RPVLIINGGPGMNSNGFEELAQKLSGQYRAILFDQRGTGQTRVPAINAEHITMAAMAA
                     DMEALRRHLGIESWVIIGHSFGGLLASYYTTLHPERVEALVYSSSGGLDLSFRQYIGE
                     SVNSKLSKKELADFQMWSQKIQQGDTSSHARLERAKALAPAYVYDKKFVPQLAIRLTQ
                     IVPVVNELVFNDLQKIGYDCKVALRSFVKPVLIIQGREDIIREETARVAQGVLPQAKV
                     VLMDKCGHYGWLDRPDIYFGELLGFLEVVPLPLSLKDSTLFDFWVGDWELSWTNANGT
                     PGKGTNLIEKILDGKVIRENFAADSGFKGTSISVFHAGTQSWRQAWADNQGGYFDFEA
                     KLEGVN"
     gene            complement(1253..2653)
                     /locus_tag="PK198_18550"
     CDS             complement(1253..2653)
                     /locus_tag="PK198_18550"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRF40801.1"
                     /translation="MRHCLLCFLLFLSAALSAQKFYFGSDLSYVNEMEDCGARYRENN
                     QIKDVYEIFADHKTNLARFRLWHTPSFYDTLNTGKRYSDFQDVRRSILRAKAAGMDVL
                     LDFHLSDFWTDPGRQLMPKAWEPVAGNLPALQDSVYQYIRGVLLSLHNDQLLPELVQI
                     GNETNKGIVLTPAADAANGPINWSRNAALFNKGIQAVRDVEAATGKKIRIALHIANPI
                     NTEWLMPAFWNNGVRDFDIIGLSYYWAWHKPVTIAQTGEIITKLKNDYPGKSVMIFET
                     GYIWTLQSNDQAANIITETHPDYHPASPQSQRQWLVDLTQEVINRGGEAVMYWEPAWV
                     STPCYTVWGQGSHQEHAAFFGFSNNAMLNGGITWPEHRYEGLPEEEPDTRKLEARFPP
                     GGSTIELELSGWTGADTVAIAIFDSLGRRIMERSLSLTNGGTETSLNWNRAATGVYFV
                     RAAQGNYKAVKKLWIP"
     gene            complement(2778..3317)
                     /locus_tag="PK198_18555"
     CDS             complement(2778..3317)
                     /locus_tag="PK198_18555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF40802.1"
                     /translation="MERIEYAGMMEALTLRPANIADLATLQQWDEQPHVLDSDPNDDW
                     NWETELLRNPEWRMQLMAEWAGRPVGFMQIIDPALEETHYWGDVPPSLRAIDIWIGAP
                     EDLGKGFGTKMMQLAVELCFSNPEVTAILLDPLASNHRAHTFYERLGFRFQEERVLGE
                     YGDQCFIYRLERGDWSGRG"
     gene            complement(3290..>3511)
                     /locus_tag="PK198_18560"
     CDS             complement(3290..>3511)
                     /locus_tag="PK198_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40803.1"
                     /translation="MQARCTPCHFPEQGKKKFLDTYAATRDNIDDIINRVELPQSDPK
                     FMPFKNKKEPLSDSLIMVLKQWKELNMPE"
     assembly_gap    3512..3626
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3673..>5425)
                     /locus_tag="PK198_18565"
     CDS             complement(3673..>5425)
                     /locus_tag="PK198_18565"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF40804.1"
                     /translation="AVSPDAGWTLVQETTLETTPDSTRIRMLRDGRTTERSFAGRAMI
                     TNNADAVAFQIFPFYGFRAPRTVGDSAVFRHVTGLGARDFVVKREAPRTVSVRSSFMG
                     KITLHLDAKNRLLSVDALGSSLNFTSQVYRKGVDFEAIKQRFAVQYSQNAGPLIVSVR
                     DTVGYQQGGQNISVNYWRPSARGRKVFGGIVPFGRFWRFGANNATEIHFSEPVLVGGQ
                     RLEAGRYSLFALPHSETNWELCFNQKTGIWGTEYNPAADVLRVPMRVERLPEHAEQFT
                     ISLQSFGNDMSLWADWEQTRLSVTIQKMPPMIPDVAPQWYVYQLRLSERHKNPANWTP
                     EDQAAIGRHVVFLDSLGKAGSLVFAGRNDLAPDDPNMLGIALVKATSLEAAKSMMAPD
                     PGVQAGIQIAHVSPYRLAISHFKNAPGIKHYPGIKMPSDAASLERLRAINRDVWQPFV
                     AAYAAGSAGQYMALHSSDFIRATIDETKDLTTYSADSQRHFEYNRQNNSRCEIAFSFF
                     ERATGASSASERGIYRYTAIAADGAQTHYYGKFHVIHRLENGRWKIAVDYDSDEDGTI
                     EQDDFEAGLPMDIFVKK"
     assembly_gap    5426..5502
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5503..5687)
                     /locus_tag="PK198_18570"
     CDS             complement(<5503..5687)
                     /locus_tag="PK198_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40805.1"
                     /translation="MKKILLKSLLMLLCSSAISVSAQGPAFRDSGVNIIRLGNDTSFV
                     HRFEVRGDSFYSQILSLP"
     gene            complement(5850..6002)
                     /locus_tag="PK198_18575"
     CDS             complement(5850..6002)
                     /locus_tag="PK198_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40806.1"
                     /translation="MGRFEAGFFYKSPYGKWEVQVLLLTENRMRLFIPQEAEVVELRK
                     TEFSFP"
     gene            complement(5989..6357)
                     /locus_tag="PK198_18580"
     CDS             complement(5989..6357)
                     /locus_tag="PK198_18580"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRF40807.1"
                     /translation="MPTTCRRHWRDCAAAMGMTGVQSLMNFLIPSGSGQALATLPVLI
                     PVGEVLGLTRQTTILAYQIGDGVTNLVNPALGGLIAMLSLCRVPFDRWLRFIFPLFLA
                     IWGIALVAVVISVAVGYGPF"
BASE COUNT         1422 a         1689 c         1753 g         1472 t
ORIGIN      
        1 gattgacgcc ctccaacttg gcctcaaaat caaaatatcc gccctgattg tcggcccagg
       61 cctgtcgcca gctttgtgtg ccggcgtgaa aaacggaaat actggtgcct ttgaatccac
      121 tgtcggcagc aaagttctcg cggatgacct tgccatccag aattttttca atcaggttgg
      181 ttcccttgcc cggcgtgccg ttggcattcg tccaggacaa ttcccaatcg cccacccaaa
      241 aatcgaataa agtgctgtct ttcagcgaaa gcggtaaggg aacgacctcc aaaaagccca
      301 acaactcgcc gaaataaata tccggccggt ccaaccagcc atagtgcccg cacttatcca
      361 tcagcaccac tttggcctgc ggcagcacgc cttgagctac gcgggccgtc tcctcgcgga
      421 tgatgtcttc ccggccttga atgatgagca cgggcttgac aaaactgcgc agcgcgactt
      481 tgcaatcgta accgattttt tgcaaatcat taaaaaccaa ttcattgact accggaacta
      541 tctgcgtgag ccgtatggcc aattgcggca caaatttttt gtcatagaca tacgccggag
      601 ccagcgcttt ggcccgttcc aagcgcgcat gactgctggt gtctccttgc tggattttct
      661 gcgaccacat ctgaaaatca gccaattctt ttttgctgag cttgctgttg acgctctcgc
      721 cgatgtattg ccgaaaactg agatcaaggc caccggaaga ggaatacacc aatgcttcca
      781 cacgttcggg gtgcaatgtg gtataataag aggccagcaa gccgccaaac gagtggccga
      841 tgatgaccca ggattcaatg cccagatgtc ggcgcagcgc ttccatatcg gcggccatag
      901 cggccatggt gatgtgctcg gcgttgatgg ccggcacccg cgtttggccg gttccccgct
      961 gatcgaagag gatggcccgg tattggccgg agagcttttg ggccagctct tcaaacccgt
     1021 tgctgttcat gcccggtccg ccgttgatga tgagtacggg gcggcctgtg ccgtaggttt
     1081 tgtagtgaat ctgcacctcg ccggaggggg caaaaccgct ctctgtctgg gcgttcagcg
     1141 caaaggccgt gcccaaaagg aagcttgcaa aaaggatgat tttgttgatt tgcatggtgt
     1201 gggtgatttt tgtaatcgct aaaagtgaca gttgtttttg aaaagcggca agctacggta
     1261 tccagagttt tttaacggct ttgtaatttc cctgcgctgc tctgacgaaa taaacgcccg
     1321 ttgccgctcg attccagttc agagaggttt ccgttccgcc gttggtcagc gacagactgc
     1381 gttccataat cctcctgccg aggctgtcga aaatggcgat ggctactgta tcggcgccgg
     1441 tccagccgct gagttccaac tcgatggtac tgccgcccgg aggaaagcgc gcttccaatt
     1501 ttcgggtatc aggttcttct tcgggcaggc cctcgtagcg gtgttccggc caggtgatgc
     1561 cgccgttgag catggcgttg ttgctgaacc cgaaaaaagc cgcgtgctcc tgatgcgaac
     1621 cctgccccca gaccgtgtag cagggcgtgc tcacccaggc cggctcccaa tacatcacgg
     1681 cctcgccgcc ccggttgatg acctcctgag tgagatcaac gagccattgg cgctgacttt
     1741 gcggtgatgc aggatggtaa tcggggtgcg tttcggtgat gatgttggcg gcctgatcgt
     1801 tgctttgtag ggtccagatg tagccggttt caaaaatcat gaccgacttg ccggggtagt
     1861 cattttttag ttttgtaatg atttcaccgg tttgcgcaat ggttaccggt ttgtgccagg
     1921 cccagtaata agacagcccg atgatatcga aatcgcgaac gccgttgttc caaaaggccg
     1981 gcatgagcca ttcggtattt ataggattgg cgatgtgcag ggcaatgcgg attttcttgc
     2041 cggtagcggc ttccacgtct cgcacggcct gaatgccttt attgaacagg gcggcattgc
     2101 gtgaccagtt gatgggaccg ttggcggcgt cggcggcagg cgtgaggacg atgcccttgt
     2161 tggtttcgtt gccgatttgt accaattcgg gcagcagttg atcgttgtgc aggctcaaaa
     2221 gcacgccgcg aatgtattga taaacagaat cttgcagcgc cggaaggttg ccggctaccg
     2281 gttcccaggc tttgggcatg agctggcgac cggggtcggt ccagaagtcg gagaggtgaa
     2341 agtcgagcaa cacgtccatg ccggcggctt tggcgcggag gatgctgcgg cgcacatcct
     2401 gaaaatcaga gtagcgtttg ccggtgttga gcgtatcgta aaaagaaggc gtgtgccaga
     2461 gccggaagcg ggcaaggttg gttttgtggt cggcaaagat ttcgtaaacg tctttgatct
     2521 gattgttttc gcggtaccgg gcgccgcagt cttccatttc gttgacgtag gaaaggtcgg
     2581 aaccgaagta gaatttctgg gcggagaggg cagctgacag aaacagcagg aaacacagga
     2641 ggcaatggcg catgttgatg aatttgatgt tcgatataac aggaagtaca acttataatg
     2701 agaaaatttg ccccaaagtt gtactttttg aatcggtaaa tataatggat ttgctatcaa
     2761 acttcctcaa ctgctgatta cccccgcccg ctccaatctc cccgctccaa ccggtaaata
     2821 aaacactggt cgccgtactc gcccaaaacg cgctcttcct gaaaccgaaa tcccaaccgt
     2881 tcatagaagg tgtgcgcccg gtggttggag gccagagggt caagcaatat ggccgtcact
     2941 tcgggatttg aaaaacaaag ttccacagcc aattgcatca tctttgtacc aaagcctttg
     3001 cctaagtctt ccggcgctcc gatccagata tctatggcgc gcaagctagg cggcacatcg
     3061 ccccaataat gcgtttcttc cagcgcggga tcaatgattt gcataaaacc gacaggccgg
     3121 ccggcccact ccgccatgag ttgcattctc cattcagggt tgcgcagcag ttctgtttcc
     3181 caattccaat cgtcgttggg atccgaatcc agcacatgcg gctgctcgtc ccattgttgc
     3241 agcgtggcaa gatcggcaat gttggccggc cgaagtgtca gtgcctccat cattccggca
     3301 tattcaattc tttccactgt ttgagtacca tgatgaggct gtcgctcagc ggttcttttt
     3361 tgtttttgaa aggcataaat ttgggatcgc tctgcggcaa ttctacccga ttgatgatgt
     3421 cgtcaatgtt gtcacgggta gcggcgtagg tgtccaaaaa cttctttttg ccctgttccg
     3481 gaaaatgaca gggcgtgcat cgggcctgca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnncaat ccatcgagtt tgcatggtga actgaattta
     3661 aggttgataa aatcattttt tcacaaaaat atccatcggc aggcccgcct caaaatcatc
     3721 ctgctcgatg gttccgtctt cgtcgctgtc gtagtcaacg gctattttcc agcgaccgtt
     3781 ctccagccgg tggatgacgt gaaacttgcc gtagtaatgc gtttgcgcac cgtcggcagc
     3841 aatggcggtg tagcgataaa tgccgcgttc ggaggcggaa ctcgcaccgg ttgcgcgctc
     3901 aaaaaacgaa aaagcaattt cgcagcggct gttgttttgg cggttgtatt caaaatgccg
     3961 ttgtgaatca gcactgtatg tagtcaggtc tttggtttcg tctatggtgg cccgaataaa
     4021 atcggagctg tgcagggcca tgtattgccc ggcgctgccg gcggcgtaag cggctacaaa
     4081 aggctgccag acatcgcggt tgatggcacg gaggcgttcc agagacgccg cgtcggaggg
     4141 cattttgatg ccaggataat gtttgatgcc gggcgcgttt ttgaaatggc tgatggccaa
     4201 gcgatacggc gaaacatgcg ctatctgtat gcccgcctgc acgccgggat cgggggccat
     4261 catggatttg gcggcttcga gcgaggtggc tttcaccaaa gcgatgccca acatgttcgg
     4321 atcgtcgggc gccaagtcgt ttcggcctgc aaataccaag cttccggcct tgcccaatga
     4381 atctaaaaat acgacgtggc ggcctatcgc agcctggtct tcgggcgtcc agttggccgg
     4441 atttttatgg cgctccgaca ggcgcagttg gtacacatac cattgaggtg caacatcggg
     4501 tatcatcggc ggcatttttt gaatggttac ggagaggcgg gtttgctccc aatcggccca
     4561 caaagacata tcattgccga acgattgtag tgaaatcgtg aattgctctg catgctccgg
     4621 caagcgttct acccgcatcg gcacgcgcag cacatcggcg gcggggttgt actcggtgcc
     4681 ccaaatgccg gttttttgat tgaaacaaag ttcccagttc gtttcgctgt gcggcaatgc
     4741 aaagagcgag taccgaccgg cctccaatct ttggcctccc accagaaccg gctcgctgaa
     4801 atggatttct gtggcgttgt tggcgccgaa tctccaaaaa cgaccaaacg gcacgatgcc
     4861 gccaaagact tttcgccccc gcgccgaggg tcgccaatag tttacgctga tgttttgacc
     4921 gccctgttga taccccaccg tatcccgaac ggaaacgatg agcgggccgg cgttttggct
     4981 gtattgaacg gcaaagcgtt gcttaatggc ttcaaaatca actcctttac gatacacctg
     5041 agacgtaaag ttgagtgatg accccagcgc atccaccgaa agcaggcggt ttttagcgtc
     5101 taaatgcaag gtgatcttgc ccataaaact gctgcgaaca ctcaccgttc ggggcgcttc
     5161 tcgtttgacc acaaaatctc tggcccccaa accggttaca tgccggaaca ccgccgaatc
     5221 gccgaccgtg cgcggcgccc ggaatccata aaacggaaaa atctggaatg ccacggcgtc
     5281 ggcattgttg gtaatcatag cacgaccggc aaaactccgc tcggtcgtgc ggccgtcgcg
     5341 gagcattcgg atgcgggtag aatcgggcgt ggtttccagc gtcgtttcct gtacgagcgt
     5401 ccatccggcg tcgggcgaaa cagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcaggga gagtatctgc
     5521 gagtaaaagc tatctccccg tacctcgaac cgatgaacaa aactggtgtc attacccaac
     5581 cggatgatgt tgacgccgct gtcgcggaaa gccgggcctt gtgctgaaac ggaaatggcc
     5641 gatgagcaca gcaacatgag cagagatttc aacaggattt ttttcatggc aacaggtgtg
     5701 gtgatgtgtg caaaaataag ttttttctca ttcgtagcgg cagagaaaga caattccgcc
     5761 ggcgcggggt tgcagttttt tttaatcgga ggaagcgagg cgcttgcttt ttaaaatcgt
     5821 caggtgaagt ttgcagccga caagccccct cacggaaacg aaaactccgt cttgcgcaat
     5881 tccaccacct cggcttcctg tggaatgaag aggcgcatgc gattttcggt gagcagcaac
     5941 acctgcacct cccatttgcc gtaaggcgat ttgtaaaaaa aaccagcctc aaaacggccc
     6001 atagcccacc gccacactga tgaccaccgc cactaatgca ataccccata ttgcaagaaa
     6061 aagcgggaag atgaaccgca gccagcggtc gaacggcacc cggcacaaac tgagcatggc
     6121 aatgaggccg cccagcgcgg gattgaccaa attcgttacc ccgtcgccga tttgataggc
     6181 caaaatggtg gtttggcgcg taagtcccag tacctcgccc accggaatga gcaccggcag
     6241 ggtggccaat gcctgcccgc tgcccgaggg tatcagaaaa ttcatcagcg actgcacgcc
     6301 ggtcatgccc atcgcggcgg cgcaatccct ccaatgcctg cgacaggtgg tgggcaatgg
     6361 tatcgctcac ctgccccatt tccaaagccg ctttgatgga ggtggcaaat cccaccatga
     6421 acgcaccggg agctaccacc gacacggaat gcagcgtgat ctcgccgaac tcgttggcgg
     6481 tgttgcggtt cagcaatcca atggcaatgg ccaacatgca gaagatgg
//
LOCUS       DAORLF010000277         6514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6394_length_6514_cov_2.128967, whole genome shotgun sequence.
ACCESSION   DAORLF010000277 DAORLF010000000
VERSION     DAORLF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6394_length_6514_cov_2.128967"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK198_18585"
     CDS             complement(<1..286)
                     /locus_tag="PK198_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40808.1"
                     /translation="MKFLSIVSAALLAVSLFSCKTAQQPAAPAAPITSVSVDGDPMLL
                     GRISRKALSEGSYAAWFNAEYNGYTVNAGALAGREADLKKVDIEIFMGTWC"
     gene            354..887
                     /locus_tag="PK198_18590"
     CDS             354..887
                     /locus_tag="PK198_18590"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRF40809.1"
                     /translation="MNLSDVLLLGDPRLYEVCEPVQRHELPDLQPAIADMAAIVLEFR
                     AKYGAGRAIAAPQIGLMKRLVVMNVERPVVFFNPELFDLSEEMMELWDDCMSFPNLLV
                     KLRRHRRCKMRFMDADWQPQLWSLEDDLSELMQHEIDHLDGILATQRAVDERALKLVV
                     RSIKQIEFRSTSIVSTK"
     gene            908..>1483
                     /locus_tag="PK198_18595"
     CDS             908..>1483
                     /locus_tag="PK198_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF40810.1"
                     /translation="MKVSNLLVSISFFMTAYLLYPTQVLSQKDTLILLACSDSIHEID
                     IFSQFDFTDVDGDGVEEMVVLHKLTQRLVVYRYTISPLGLPMFSIIVEEKLNKPLYFD
                     ICDWNQDGIEDIIVSEMSSSRDQAVITFIPKNQIFSFSTMKQSVKTQFELGASESFSF
                     FKFIDLNNDGKLDFVGKLIYYIHETTDFDIEK"
     assembly_gap    1484..1518
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1519..2195
                     /locus_tag="PK198_18600"
     CDS             <1519..2195
                     /locus_tag="PK198_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF40811.1"
                     /translation="QYVYSETTKQLIFTDFDGDKDIDLLAPNPSYGDHLWLENIDGSI
                     VSTKPHIFLKNIGNMEFNSGDIDNDGDDDLIYLTDCFCDKDHKLYVFDSIPKDHSDLR
                     ECLMAVFYIGKAQFKDSAEFIFEFPPYANSLDVADFDNDGLYELILTNDVVPKEIFKY
                     NPSTNDLESLHYVSHLHDPVYLPYDYFKDDRLTFGWQQRFERDIDGDGKIEKFLLYFT
                     LLYLIK"
     gene            2264..>3352
                     /locus_tag="PK198_18605"
     CDS             2264..>3352
                     /locus_tag="PK198_18605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF40812.1"
                     /translation="MRNISFTIALSALLLQACQPANSDYQQQAANPEHYHRSMKQLTD
                     VIVHDIFSPPVAARIYSYANAAGYEAAHAGKLSLANRLNGLNGTPQPQPGQEYCLPLA
                     SVQAFLTVGKALIFSEEKMDSFQNQILAEYKKMGIPTQVYNRSVQYGDEVAKHILAWA
                     ATDNYKQSRTFPKYSISPDPAVWQPTPPAYMDGIEPHWNTIRTFLLDSAAQFAPPPPT
                     PFDLTPGSPFHKELMEVYQALDAPDRPEREAIAQFWDCNPYKTNQVGHVMFSTKKITP
                     GGHWMNIAGLAARKAGSDFEQTTRAYAFTAVTLHDAFVSCWDEKYRSKLVRPETVINR
                     SLDENWLPLLQTPPFPEYPSGHSTQSGAA"
     assembly_gap    3353..3442
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3759..3794
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3795..4071
                     /locus_tag="PK198_18610"
     CDS             <3795..4071
                     /locus_tag="PK198_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40813.1"
                     /translation="QEDRLIELDPVHKADGETEQAWAVEGDWHDIPSLKAMLYEDAVA
                     LKEKLETMEQGSFVAIKEAFKKAMPHQYQFLKELKEVLLDRNLTKNM"
     gene            complement(4098..>5489)
                     /locus_tag="PK198_18615"
     CDS             complement(4098..>5489)
                     /locus_tag="PK198_18615"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HRF40814.1"
                     /translation="AENINYISLDRYYTVCAAYGLNDKDRKSQLQLSAYLHDLGAILH
                     FQDDGALRETVILRMTWATDAVFKTYDSPLVNAGDRSGFFNKRDIGQIWHDEMYVDKQ
                     EQLLALMLRFKLCYRVGETDDYIMPELLPFAQPDYAASIPSLHPDELSHAPALRYSYD
                     FMPRGIFARLAVEVHQLIAHGQKFIWREGTILQQGDSYALITETYGNREIVVRSIGKR
                     RRELTTQATIHLDRIHASFPRLRVQKLIPCNCPACRILQAPHYHDYEKLIEFLENGRT
                     KTPCDNRPYHDVDIKSLLEEVFGKDVNVPATPDKKDKSSQSTPKKVFLSYSHDKIEDA
                     KRLKTALAVQAKTGKINFWYDQGILPGAEWKKEIENKLGEADIIVLLLSPEFWASDFI
                     WEQELPLVAARYAAGAQVLCVMLTDNDFQETEWSGLQAVPQLKGRLTPISRWPDKDEA
                     WQEVAGALKRMLG"
     assembly_gap    5490..5536
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5537..>6514)
                     /locus_tag="PK198_18620"
     CDS             complement(<5537..>6514)
                     /locus_tag="PK198_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HRF40815.1"
                     /translation="PVSRVMPIRKEAPLCYLWHEGHLAGLNLAGAGLDDEIWRQILAE
                     IGAEAAHLQALNLRDNKLTAFELPPAMRGLRYLDLSNNQLREFAPPAEAVAQLEHLWL
                     YGNENLATPPPEIVAQGRYAIAGYFKKRLEGVRFLREAKLLLLGESGAGKTTLALKMK
                     KIDSPLPGKDESTRGIAVETLRIGEGDQAFDIHLWDFGGQQIYHGTHRFFLTKRSLYV
                     VLADNRKQNTDFNYWLQMAELYGDGSPLYIVVNKTDGRPPYPNLGGMRARFDFLREML
                     AANLDTLGEYDKSENAAAVNTADTQKVINTLTNRICELEHVTQSLPLSWF"
BASE COUNT         1546 a         1594 c         1609 g         1557 t
ORIGIN      
        1 aacaccaggt gcccataaaa atctcgatgt ccactttttt caaatcggct tccctgcccg
       61 ctaatgcccc cgcattgacc gtgtagccat tgtactcggc attgaaccag gcggcataag
      121 aaccctccga taatgccttg cggctgatgc ggcccaagag catgggatcg ccgtcaacgg
      181 aaacggatgt gatcggagcg gcaggggcgg caggctgctg agcggttttg caagaaaaca
      241 gggagaccgc caaaagggcc gccgaaacga tggataaaaa cttcatggta tctgattttg
      301 agggggcaaa gttagcattt tgttttcact gaccggctgc ctacttttgc cgcatgaatc
      361 tgagcgatgt actcctgctg ggcgatccgc gattgtacga ggtgtgcgaa ccggtacagc
      421 gacacgaact gcccgatctg caacccgcta ttgccgacat ggccgctatt gtgctggaat
      481 tcagggccaa atacggcgcc gggagggcca ttgccgcgcc tcaaatcggc ctgatgaagc
      541 gcctcgtggt gatgaatgtg gagcgcccgg tggtattttt caatccggaa ctcttcgacc
      601 tcagcgagga aatgatggag ttgtgggacg actgtatgag ctttccgaac ctgttggtaa
      661 aactgcgccg gcaccggcgt tgcaaaatgc gtttcatgga cgccgactgg cagccgcagc
      721 tctggtctct ggaagacgac ctttcggaac tgatgcagca cgagattgac cacttggacg
      781 gcattctggc tacgcaacgg gctgtggatg agcgggcgct gaagttggtg gttcgtagta
      841 taaagcaaat cgaatttaga agtacttcaa tcgtctcaac taagtaaaca ttaaatataa
      901 catcaaaatg aaagtatcca atctgcttgt atctatcagt tttttcatga cagcatactt
      961 actgtatcct acgcaggtac tatcacaaaa agatacgcta atacttctgg cttgtagtga
     1021 ttctattcat gaaattgata ttttcagtca atttgatttc acagatgtgg atggggacgg
     1081 agtggaagaa atggtcgttt tgcacaagct tacccaacga ttggttgtat atagatacac
     1141 catttccccg ctggggctac ctatgttttc cattatagtt gaggagaaac tcaacaagcc
     1201 actctatttt gatatctgcg attggaatca ggacggaatt gaagacatta ttgtaagtga
     1261 gatgagttct tcgagggatc aggctgttat tactttcatt cccaaaaacc agattttttc
     1321 attctctacc atgaagcaaa gtgttaaaac tcaatttgag ttgggagcca gtgaaagttt
     1381 tagtttcttc aaattcattg acttaaataa tgacggaaaa ttggattttg taggaaaact
     1441 tatatattat attcacgaaa ctacagattt tgacatcgaa aagnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnat caatatgtgt atagcgaaac caccaaacag ttgatattta
     1561 cagatttcga tggtgataag gatatagact tattggcacc taatcctagt tatggtgatc
     1621 atctttggct cgaaaatata gatggctcaa ttgtgagtac gaagccgcat attttcctta
     1681 agaacatcgg gaatatggag tttaactccg gagatattga taacgatggg gatgacgatc
     1741 tcatttactt aacagactgc ttttgtgata aagatcacaa attgtacgtt tttgattcga
     1801 ttcccaaaga ccatagtgat ttgagagaat gccttatggc tgtattttat ataggaaagg
     1861 cacaatttaa ggattcagca gaattcatct ttgaatttcc accttatgct aactcactgg
     1921 atgtggctga ttttgacaat gacggtctct atgaattgat acttacaaat gatgtagtac
     1981 ctaaggaaat attcaaatat aatcccagca ctaatgattt ggagtcactc cattatgttt
     2041 cccaccttca tgatcccgtt tatttaccct acgattattt caaggatgac agattaacct
     2101 ttggttggca acaaaggttt gaacgggata ttgatggcga tggaaaaata gaaaaatttc
     2161 ttctttactt tacacttttg tatctcatca aataaatatt ggcacaaaat ccggatttta
     2221 agttttattt cagaataatt ttcttaatac aaagttaacc atcatgagaa acatctcctt
     2281 taccatagca ctgtcggcac tgctgttgca agcctgccaa cccgccaact ccgactacca
     2341 acaacaagcc gccaacccgg agcactacca ccgctcgatg aagcaactga cggatgtgat
     2401 cgttcacgac attttctcgc cgccggtggc tgcccgcatt tacagctatg ccaacgccgc
     2461 cgggtacgaa gccgcacacg ccggcaagct cagcttggcc aacaggctca acgggctgaa
     2521 cggcacgccc caaccacagc ccggccagga gtattgcctg cctttggcga gtgtgcaagc
     2581 cttcctcaca gtgggcaaag cgctcatttt ttcggaggaa aaaatggact ctttccaaaa
     2641 tcaaatattg gccgagtaca aaaaaatggg cataccgacg caggtgtaca accgctctgt
     2701 tcaatacggc gatgaagtgg ccaagcatat actggcatgg gccgccaccg acaactacaa
     2761 acaaagccgc acttttccca agtactccat ttcgcccgac cccgccgtct ggcaacccac
     2821 gccgcctgcc tacatggacg gcattgagcc gcactggaac accatacgga ccttcctgct
     2881 ggattccgcc gcgcagttcg cgccgccgcc gcccacgccg ttcgacctca ctcccggcag
     2941 tcctttccac aaagaactca tggaggtgta tcaggcgctg gatgctcccg accgccctga
     3001 acgtgaggcc attgcgcagt tttgggattg caatccctac aaaaccaatc aggtgggcca
     3061 cgttatgttt tccaccaaaa aaataacgcc cggcggccac tggatgaaca ttgccggact
     3121 ggcagcgcgc aaggcaggca gcgatttcga gcagactacg cgcgcctacg ccttcactgc
     3181 cgtcactttg cacgatgctt ttgtcagttg ttgggatgag aaataccgca gcaagctcgt
     3241 tcgccccgaa accgtcatca accgcagttt ggacgaaaac tggttgccgc tgctgcaaac
     3301 gccgcccttc ccggaatatc cgtccggcca ttcgacgcag tcgggggccg cgnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnttttgcac acgccgccga tgaggcttcc atgagccgta
     3481 tgtatggggg catccactac cgaccggcca ttgtcaacgg cgtagagcag ggtggccaaa
     3541 taggcaagct ggcggtatcg aaattgccgc tgagcgccca aacgggtatg aaataatcaa
     3601 ttggtaaacc ttttagcacg ctccgcgttg tgcttgcgga agtaatttta ggctcatgaa
     3661 tctgaaagaa aaagcaaagc tcatcatcta ccgtttccgc gaacgcggtc tcgaaatttt
     3721 cctcgttccc gacgaaaatc aatggacgct gcccgaagnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnngcagga agaccgcctg atcgaattgg accccgttca caaagccgac
     3841 ggcgaaaccg aacaggcctg ggccgtagag ggcgactggc acgacatccc ctcgctcaaa
     3901 gccatgctgt acgaggatgc cgtggcgctg aaggaaaaat tggaaacgat ggagcaaggt
     3961 tcttttgtgg ccatcaaaga agccttcaaa aaggccatgc cgcaccagta tcagtttctc
     4021 aaggagctga aggaggtgct tttggataga aatttgacga agaatatgtg agggggttct
     4081 cgctcacagt aaacacttca ccccaacatg cgcttcaagg ctccggccac ttcctgccag
     4141 gcctcgtcct tatccggcca gcgactgatg ggcgtgaggc ggcccttgag ttgcggaact
     4201 gcctgtaaac cagaccactc cgtttcctga aaatcattat cggtgagcat aacgcacagc
     4261 acttgtgcgc ccgccgcgta acgcgccgcc accaacggaa gttcttgttc ccagataaag
     4321 tcggaagccc agaactccgg actcagcagc agcacaatga tgtcggcctc gcccaatttg
     4381 ttttcaattt cctttttcca ctctgcgccg ggcaggattc cctgatcgta ccagaaattg
     4441 attttgcctg ttttggcctg caccgccaat gccgttttta ggcgtttggc gtcttcaatc
     4501 ttgtcgtggg agtaggaaag gaagactttt ttgggggtgg attgggagct tttatccttc
     4561 ttatccggtg tagcgggaac attcacatcc ttcccaaaaa cctcttccaa taagcttttg
     4621 atatccacgt cgtggtacgg gcgattgtcg cagggcgttt tcgtccggcc attttcgaga
     4681 aactcgatga gtttttcata atcgtggtaa tgcggcgcct gaagtatccg gcaggcagga
     4741 caattgcagg gaatcaactt ttgtacccgc agccggggaa atgaagcatg aattctgtct
     4801 aaatgaatgg tagcctgggt agtgagttcg cggcggcgtt tgccgatact ccgcaccaca
     4861 atctcccggt ttccgtaggt ttccgttatc agtgcgtagc tgtcgccctg ctgaagtatc
     4921 gtaccctcgc gccagatgaa tttttgtccg tgtgcaatga gttgatgcac ttccacggcc
     4981 aagcgggcaa aaataccgcg cggcatgaag tcgtagctgt agcgcaaggc cggggcatga
     5041 ctcagttcat cggggtgaag ggaagggatg gatgctgcgt aatcgggttg tgcgaacggc
     5101 agcagttcgg gcatgatgta gtcatctgtt tcaccaacgc gatagcagag tttgaagcgg
     5161 agcatgagcg cgagcagttg ttcctgctta tccacataca tttcatcatg ccatatttgc
     5221 ccgatgtctc gtttgttgaa aaaaccagag cggtcgccgg cgttgaccaa gggactgtca
     5281 taagttttga aaacggcgtc tgtggcccag gtcatgcgca aaatgacggt ttcccgcaat
     5341 gcgccatcgt cctgaaaatg aaggatggca cccagatcgt gcaggtaagc gctgagttga
     5401 agctggcttt ttcggtcttt gtcgttcagc ccataagcgg cacaaacggt ataatagcga
     5461 tccagagaga tgtaatttat attttcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnaaac caggacagcg gaagcgactg cgttacatgc tccagttcgc
     5581 agatgcggtt ggtgagggtg ttgatcactt tttgggtgtc ggcggtattg acggcggcag
     5641 cattctcaga tttatcatat tcgcccaagg tatccaggtt ggcggcgagc atttcccgca
     5701 ggaaatcgaa tcgcgcccgc ataccgccga gattggggta cggcgggcgg ccatctgttt
     5761 tgttcaccac gatgtacagc ggactgccgt caccgtagag ttctgccatt tgcagccaat
     5821 agttgaagtc ggtattttgc ttgcggttgt cggccagcac gacatacagc gatcgcttgg
     5881 tgagaaagaa gcgatgggtg ccgtgataaa tctgctgccc gccgaaatcc cagaggtgga
     5941 tatcaaatgc ctgatctccc tcgccgatgc gcagggtttc caccgctata ccgcgcgtac
     6001 tttcgtcttt tcccggaagc ggactgtcta ttttcttcat tttcagggcc agagttgtct
     6061 tgcccgcacc gctttcgccg agcaacaaga gcttggcttc gcgcaaaaac ctgacgcctt
     6121 cgagccgttt tttgaaatac cccgctatcg catacctccc ctgcgccacg atttccggcg
     6181 gcggtgtggc caagttttcg ttgccgtaca accacaggtg ttcgagttgc gctacggctt
     6241 cggcgggcgg agcgaactcc cggagttgat tgttgctgag atccaaatac cgcaagccgc
     6301 gcatggccgg cggcagttca aaggcggtga gcttgttgtc gcggaggttg agggcctgca
     6361 agtgcgcggc ctctgctccg atctccgcga gtatttgccg ccatatctcg tcatccaagc
     6421 cggcgcccgc cagattgagt ccggccaaat gaccctcgtg ccacaggtag cacagcgggg
     6481 cttccttgcg gatgggcatc acgcgggata cggg
//
LOCUS       DAORLF010000278         6482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6449_length_6482_cov_1.972149, whole genome shotgun sequence.
ACCESSION   DAORLF010000278 DAORLF010000000
VERSION     DAORLF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_6482_cov_1.972149"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK198_18625"
     CDS             complement(<1..625)
                     /locus_tag="PK198_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40816.1"
                     /translation="MKKVIFPALLAVLVFGACSTDFELEAPWKDIPVVYGFLNLQDTA
                     HYIRVEKAFLEPGGNANSIAQIVDSLYYDPAQITVQLQKRSTGQRFTLQRVDGNAEGY
                     PRQSGPFAQAPNYLYKIRASALNLVAEQDIRLIIERGNGLPSVIADATVLGPLTPRPN
                     LPADPLNLDYTRTLVFLWDVPVSGAIFDIRMNIHYREEESAGNFVLKT"
     gene            complement(808..1137)
                     /locus_tag="PK198_18630"
     CDS             complement(808..1137)
                     /locus_tag="PK198_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40817.1"
                     /translation="MYNLQRNRRHKLFFFFPLLMAAVAFLFGGVVMWLWNAILPAAVG
                     ANPLNYWQALGLLVLSRILFGGFRFGGRPHRHTPAPWRDKWANMNAEEREQFKSEWKK
                     RCGRSED"
     gene            complement(1141..1707)
                     /locus_tag="PK198_18635"
     CDS             complement(1141..1707)
                     /locus_tag="PK198_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF40818.1"
                     /translation="MTDNSSMAQRQNILQIVRGYGQRLFGFIRQRVPNDADAEDVLQD
                     VWFQLSQLVDVESVENIGAWLFRVTRNKLTDRSRKRLPENLDDFAYEDEDGETHFAGD
                     LLLADFVTPEDEYLRGLFWDTLFAALDELPENQRNVFIWNELEDQTFQQIADRTGDNI
                     KTLISRKRYAVQHLRRRLEDFYQELINY"
     gene            1841..2989
                     /locus_tag="PK198_18640"
     CDS             1841..2989
                     /locus_tag="PK198_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HRF40819.1"
                     /translation="MKIHFILAFMAVAGLLTACKNINPPKPQILRKATELPLSQIKLP
                     EGFQIDVYAENVTNARSLEWTPKGTLFVSTRDKGSVYALRDTNNDYYADVMHVIAKGL
                     KMPNGVAFRDGSLYVAEVSRILRFDDIENRLDNPPAPVVVYDKYPTETHHGWKFIAFG
                     PDGKLYVPVGAPCNICNRPEEIYAAITRINPDGTGLEVVQRGIRNTVGFTWHPETKEL
                     WFTDNGRDWMGDDMPACEVNHAPRDFMHFGYPFCHQGDTPDPEFGALRPCFDFTPPAQ
                     KLGAHTAPLGIEFYTGKQFPKAYRNQIFVAEHGSWNRSKKVGYRVMLLRLDGSKVVSA
                     EPFAEGWLDVAKDDVWGRPVDIELLPDGSMLVSDDYADAIYRISYVGK"
     gene            3088..3669
                     /gene="msrA"
                     /locus_tag="PK198_18645"
     CDS             3088..3669
                     /gene="msrA"
                     /locus_tag="PK198_18645"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRF40820.1"
                     /translation="MKTNADMPKDQLPKEGTEIATLGGGCFWCVEAVYQDLAGVLSVS
                     SGYSGGTAETANYKAVGSGRTEHAEVIQIVFDPKVVSFADILEVFWHVHDPTTLNRQG
                     NDVGPQYRSVIFYHSEEQKRIAQESKKKVATQLWDKPIVTEIAPFKAYYEAEDYHQDY
                     YNTVGDRNPYCTAVITPKVQKFRKVFKDKLKEN"
     gene            complement(3695..>4010)
                     /locus_tag="PK198_18650"
     CDS             complement(3695..>4010)
                     /locus_tag="PK198_18650"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRF40821.1"
                     /translation="KIERLAPRFLNETEMACITTDHKLLHLHLFWGAKESLYKAYGRK
                     ELDFREHIPIAPFIPNPAGGTCSGRVCKDDFEADFDIRYAVSEGDFVWVYAVQRMEKL
                     AR"
     assembly_gap    4011..4049
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4050..4358)
                     /locus_tag="PK198_18655"
     CDS             complement(<4050..4358)
                     /locus_tag="PK198_18655"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40822.1"
                     /translation="MALMHLDSIAADIHLAFWKIEEPEEWFLQQLALSPLEQAEHVKI
                     VAAGRRMEWLAARHLVRVLAWNTGEPYIFQKDGYGKPYLLNSGKHISISHSHGVCAAM
                     I"
     gene            complement(4365..>4693)
                     /locus_tag="PK198_18660"
     CDS             complement(4365..>4693)
                     /locus_tag="PK198_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF40823.1"
                     /translation="GYEVERVFYFSESVLKAAYRGQGAGLAFFREREQWARNLGSFDF
                     LAFCAVVRPEEHPLRPAGHVPLDAFWQHRGFQRTDMLCRMYWKDRDEPAETEKQLRFW
                     IKPLDK"
     assembly_gap    4694..4790
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5163..5208
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5209..5511)
                     /locus_tag="PK198_18665"
     CDS             complement(<5209..5511)
                     /locus_tag="PK198_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40824.1"
                     /translation="MMDILVRRATLHDVDALQVIGRQTFAETFSSGNSEENMRQYLEE
                     SFSIEKLTAELSDENAEFYFALINSRVVGYLKLNFGASQTELKDHKAVEIERIYVLH"
     assembly_gap    5623..5718
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5719..6354)
                     /locus_tag="PK198_18670"
     CDS             complement(<5719..6354)
                     /locus_tag="PK198_18670"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRF40825.1"
                     /translation="MSMANARAVSEICRSHGVLFILDACRVAENAYFIRHREEDYADK
                     SYRQIAQEMFSLADGCIMSAKKDALVNMGGFLALKDLDLAVQCRTVLIITEGYSTYGG
                     LSGRDMEAIAIGLEEVFDADYLHYRIKSTGYLGEKLREMGVPLIWPIGGHAVYVDAKA
                     FYPHIPVEQFPGQSLVCELYLKAGIRSCEIGSVMFGKTDADGQLVPAAMELV"
BASE COUNT         1455 a         1672 c         1624 g         1453 t
ORIGIN      
        1 gtgttttgag cacaaaattg ccggcgctct cctcctctct gtaatgaatg ttcatgcgga
       61 tgtcgaagat ggcgcccgaa accggcacat cccacagaaa tacgagggtg cgggtgtagt
      121 ctaagttgag cggatcagcg ggcagattgg gacgcggcgt cagggggccg agcacggtag
      181 catcggcgat tactgagggc agcccgttgc cccgctcaat gatgaggcga atgtcttgct
      241 ctgcaacgag attgagggca gaagctctga ttttgtacaa ataattgggg gcctgcgcaa
      301 acgggccgct ctggcgcggg tagccttctg cattgccgtc caccctttgg agcgtaaagc
      361 gttgtccggt gcttcttttt tgcaattgaa cggtgatctg tgccggatca tagtagagcg
      421 agtccacgat ttgcgcgatg ctgttggcat tgccgccggg ttcgaggaag gctttttcta
      481 cccggatgta gtgggcagtg tcctgcaaat tcaggaatcc atacaccacg gggatgtctt
      541 tccagggggc ctctaattca aaatcggtag agcaggcgcc gaaaaccaaa acggccaata
      601 aagccgggaa aatcactttt ttcatcagaa tgtgctttga gggcgcaaag gtacgcgtaa
      661 aacgggaaaa ggaggagggc ggaagacact acgcggcttt gaaaactttg taatgtcttg
      721 agaattgcgg aatattcctg caactccggg tgtgtgacaa gaggggggag ttgcccgttc
      781 tgcatctgtt catcgccggg ggcaaactca gtcttcgctt cgtccgcacc tttttttcca
      841 ttccgatttg aactgctcgc gctcttcggc attcatattc gcccatttgt cgcgccaggg
      901 agccggtgtg tgccggtgtg gtctgccgcc gaagcggaat cctccgaaca aaatacggct
      961 caatacgagc agccccagcg cctgccaata gttcagtggg ttggcgccga ctgctgccgg
     1021 caaaatggca ttccaaagcc acatgactac tccgccgaac agaaaagcga cggctgccat
     1081 gagcagtggg aaaaagaaaa agagtttgtg gcggcggttc ctttgcagat tgtacatggc
     1141 ttaatagtta atgagttcct gataaaaatc ttcgaggcgg cggcgcagat gctgcacggc
     1201 atagcgcttg cgcgagatga gcgttttgat attgtcgcct gtgcggtcgg caatttgttg
     1261 aaaagtctgg tcttccaatt cgttccaaat gaatacgttg cgctgatttt cgggcagttc
     1321 gtccaaggcg gcgaaaaggg tgtcccaaaa taaaccccgg aggtattcat cttcgggcgt
     1381 gacgaaatcg gccaacaaaa gatcgccggc aaaatgggtt tcaccgtctt cgtcttcgta
     1441 ggcaaaatcg tccagatttt cgggcaggcg tttgcgggag cggtcggtga gtttgttgcg
     1501 cgtgacgcga aaaagccagg cgccgatatt ttctaccgac tccacatcta ccaattgact
     1561 gagctgaaac caaacatcct gcagcacgtc ctctgcatcg gcgtcgttgg gcacccgctg
     1621 tcggatgaag ccaaagaggc gttggccgta gccacgcacg atttgtaaga tgttctgccg
     1681 ttgggccata gatgagttat ctgtcacgaa gatgcaactt ttttcgagga agacgaatga
     1741 agcgggattt tactttaaag cgcggcaaca aaaaagcgct ttccgcaacc aactcccacc
     1801 cccggcagtt ttcttaacct gaaaccaacg caccactgcc atgaaaatcc atttcatcct
     1861 tgcctttatg gctgtcgccg gattgttgac ggcctgcaaa aacatcaatc cgcccaagcc
     1921 gcagatactc aggaaagcta cggaactgcc tttgagccag atcaaactgc ccgaaggctt
     1981 tcagatagac gtgtatgctg aaaatgtgac caatgcccgc tctttggaat ggacgcccaa
     2041 aggcacgctt tttgtaagca cccgcgacaa aggctccgtg tatgccctcc gcgacaccaa
     2101 caacgactac tatgccgatg tgatgcacgt cattgccaaa ggtctcaaaa tgcccaacgg
     2161 cgtggccttc cgcgacggca gtctgtatgt ggccgaggtg agccgcatcc tccgtttcga
     2221 cgatatcgaa aaccgtttgg acaatccgcc tgcccccgta gtggtgtatg acaagtaccc
     2281 caccgaaacc catcacggct ggaaattcat cgcctttggc cccgacggca agctctatgt
     2341 gccggtaggc gcgccctgca atatctgcaa tcgcccggag gaaatttacg ctgccatcac
     2401 ccgcatcaac cccgacggca cgggattaga ggtcgtacag cgcggcattc gcaacacggt
     2461 gggcttcacc tggcaccccg aaacaaagga gctttggttt accgacaacg gccgcgactg
     2521 gatgggcgac gatatgccgg cctgcgaggt caaccatgcg ccccgcgatt tcatgcactt
     2581 cggctacccg ttttgccatc aaggggatac acccgatcct gaattcggcg cgctgcggcc
     2641 ctgcttcgat ttcacgccgc cggctcagaa gttgggcgcg cacacggcgc cgttgggcat
     2701 agagttttac accggcaagc agtttcccaa agcctatcgc aaccagattt ttgtggcaga
     2761 gcacggctcc tggaaccgca gcaaaaaagt cggctaccgc gtgatgttgc tccgcttaga
     2821 cggtagcaaa gtggtcagcg ccgagccgtt cgcggagggc tggttggatg tcgccaaaga
     2881 cgatgtctgg ggccgcccgg tggacattga actgttgccc gatggctcta tgctggtatc
     2941 tgatgattat gccgatgcca tttaccgcat ctcttatgtc gggaaatgat tcatttttca
     3001 ctcaaaaaaa acacagtgaa catgcgctct ttagtattgt ttcttagcct tttcgcgtgt
     3061 gcttgcacgg ctcaacaaaa tcaaaccatg aaaaccaacg ctgatatgcc caaagatcaa
     3121 ttgcccaagg aaggcacgga aatcgccacg ctcggcggcg gctgtttctg gtgtgtggag
     3181 gctgtttatc aagacctcgc cggggtgctt tccgtctctt ccggctattc gggcggcacg
     3241 gctgaaaccg ccaattacaa agcggtgggc agcggccgaa ccgaacacgc agaagtgatt
     3301 cagattgttt tcgaccctaa agtcgtcagt tttgccgaca tcctcgaagt gttctggcat
     3361 gtacacgatc ctaccaccct caaccggcag ggcaacgatg tggggccgca gtaccgctcg
     3421 gtcatttttt accacagcga agagcaaaaa cgcattgcgc aggagtcgaa aaagaaggtg
     3481 gccacacaat tatgggacaa acccatcgtg acggaaattg cgcccttcaa agcctattac
     3541 gaggcggaag attaccacca ggattactac aacacggtgg gcgaccgcaa tccgtattgc
     3601 acggctgtca tcacaccgaa agtgcagaag ttcaggaagg tgttcaagga taagctgaag
     3661 gagaactgat gagccaatct ggcattccgg agattcaccg cgccaatttt tccattcgtt
     3721 gcaccgcgta cacccacaca aagtcgccct ccgatacggc atatcgaata tcgaaatcgg
     3781 cctcgaagtc atctttacaa accctgccgc tgcatgttcc gccggcaggg tttggtataa
     3841 atggggctat gggaatgtgt tcccggaagt cgagttcctt gcgaccgtaa gctttgtaca
     3901 gcgattcttt ggcgccccaa aacaggtgca agtgcaggag tttatggtcg gttgtgatgc
     3961 aggccatttc agtttcgttc aggaagcggg gcgcgaggcg ttctattttg nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atcatggcgg cacacacgcc gtgcgagtga
     4081 ctgatggaga tgtgtttgcc ggagttgagt aagtagggtt ttccgtagcc gtctttttga
     4141 aaaatgtatg gctcgccggt attccaggcc agcactcgta ccagatgccg cgcagcgagc
     4201 cattccatgc gacgaccggc ggcgactatt ttgacatgct cggcctgttc gagcggagag
     4261 agggccaatt gttgcaaaaa ccactcctcc ggctcttcta ttttccagaa tgccagatgg
     4321 atatctgcgg cgatggaatc caagtgcatc agtgccataa gcggctattt atcaagaggt
     4381 ttgatccaaa atctcagttg tttttccgtc tcagccggct cgtcgcggtc tttccagtac
     4441 attcggcaca gcatgtcggt gcgctgaaag ccccggtgtt gccaaaaagc atccaaagga
     4501 acatggccgg cgggcctgag cggatgctcc tccggcctta ccacggcaca aaatgccagg
     4561 aaatcaaaac tgcccagatt gcgcgcccac tgctcccgct ctctgaaaaa tgccagtccg
     4621 gcgccctgcc cgcggtacgc ggctttgagc accgactcgc tgaaatagaa cacgcgctcc
     4681 acctcgtagc cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtgccagca
     4801 cgctgtcttt ggcctgcgag aaggttttca ggtagcgccg ttcgtagctc atatcgccgt
     4861 cgtagaggta gggaaactcg tgaaatacct cgatgcgcag gcgggctacg tcgtcaataa
     4921 aaggcgcaat ttgctcgccg tgaactgttt ggatcatcaa agacatgcca tgctacttaa
     4981 aacattacgt caattgcagt ttcatcataa tgtcggtctg ctcgtcgtcg cccaatttga
     5041 agatatgttt gtcaaactca acaaaaccgt tttttttata aaaatgaatg gctctggggt
     5101 tttcctccca aacacccagc cacacatatt cggccttttt ttgccgagat atttgcaatg
     5161 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ggagtacata
     5221 gatgcgctca atttctactg ccttgtggtc tttcagttca gtctgcgatg ctccgaaatt
     5281 cagtttcaaa tacccgacta cccgactgtt gatgagggca aaatagaact cggcattttc
     5341 atccgaaagc tccgccgtca gtttttctat agaaaagctc tcctccaaat actgccgcat
     5401 attttcttcg ctgtttccag aggaaaacgt ctcggcaaaa gtctgtctgc caattacttg
     5461 taaagcatca acatcatgga gggttgctct tctgacgagg atatccatca ttgctttcgg
     5521 tatttattac aatgttgttg tctcatccct tcaccggctc aaaccgcgcc gtgaaatgcc
     5581 gcaaaaacgg cggttcatac acgatgcgat agccgggcgc ttnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnca ccaactccat cgctgccggt accaactgcc catcggcatc
     5761 ggttttgccg aacatgaccg aaccgatctc gcaggaacga atgcctgcct tgaggtacaa
     5821 ctcgcacacc aaagactgtc ccggaaactg ttctaccgga atgtgcgggt aaaaggcttt
     5881 ggcatccaca tacacggcat ggccaccgat gggccatatc agcggtacgc ccatttcgcg
     5941 gagtttttcg ccaaggtagc ccgtgctttt gatgcgataa tgcagataat cagcgtcaaa
     6001 aacctcctcc agaccaatgg caatggcctc catgtcgcgc cccgaaaggc caccgtaagt
     6061 actgtagccc tcggtgatga tgagcacggt gcggcattgt accgccaggt ctaagtcttt
     6121 gagtgccaaa aatccgccca tgttgaccaa ggcgtctttt ttagcgctca taatgcagcc
     6181 gtcggccagt gaaaacattt cctgagctat ctgccggtaa gatttatcgg catagtcttc
     6241 ctcgcggtgg cgaatgaagt aggcgttttc ggccacgcgg caggcatcga ggataaaaag
     6301 cacgccgtgg ctgcggcaaa tttcgctcac cgcacgtgca ttggccatgc tcacaggctg
     6361 accgagtcat atttgctcac aagtgcgaat ttccaccttc ccagccaata gagtatccct
     6421 acttgatctt atcctcaatt tgcttgtaaa catccctgcc tttttcgtca cgttttacag
     6481 cc
//
LOCUS       DAORLF010000279         6457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6486_length_6457_cov_2.083255, whole genome shotgun sequence.
ACCESSION   DAORLF010000279 DAORLF010000000
VERSION     DAORLF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_6457_cov_2.083255"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..2111
                     /locus_tag="PK198_18675"
     CDS             174..2111
                     /locus_tag="PK198_18675"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRF40826.1"
                     /translation="MKQLLPALLLLLLPALTNAQAVRRVMVESFTQASCGPCASQNPA
                     FNALLQSNGDKVVLLKYQTNWPGYDPMNEQNPSEVAARVTYYQTTSVPNVRLDGVQNA
                     GVASAVTQAMINNRLNQSTPIDMTLDHQISAGFDSIFIQCKIKNLGATEFNPGGVVLH
                     TAVTEKQILFPTAPGSNGEVDFKSVMRKMLPNASGAALAAIPAGDSVTITYAAALPSY
                     IYNFARIGVVAFVQKTSDRQVYQAVESFPKPLQGNIVDVAVYPNSIGPDGFCNYALTP
                     QVDVANEGDTPITSMDVSYTLNGAAAVTQEWEGTLAPGGTVTITFPEIAITPGAAEVD
                     YDVSNANGDHFEYNSLNELLSSEIYYTLSNEPVGTQITEGMESTPVRAVPTGTLAIKD
                     AEDHFMVVNKGVLSSSVTQEVGGYGASTKSIFSNCYGWNDPGAEAHLIFEKIDLSGST
                     NNYVKFDYAHARYQTTSERMLVSISTDCGDTWTTIWNKAGAQLATAAATNNFYIPAVT
                     HWRTDSVALTNFDGNGEVLVRFTVVTAYGNNIYLDNIRVYQPATSAADEPGLLAGKVQ
                     VYPNPASDLARIDVQLASASAVNISVFDLSGKLVHTIANGNLLSAGNHQFNWAPQAQG
                     VFLVRVATDFGAVTEKVTVVK"
     gene            complement(2270..3667)
                     /locus_tag="PK198_18680"
     CDS             complement(2270..3667)
                     /locus_tag="PK198_18680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF40827.1"
                     /translation="MIRYLLQRLLWFLPSLLLISLLAFALSKSAPGDPVELRNSAGSA
                     PIASGPAQADRLYAETARLMGLDKPAFYFSIAPAAYPDTLHLIARQDRRQTLKRLVGQ
                     YGNWPLIEAYYHSIQQAERSMAAGPSPLAGPLRDLYLQHQDGAIRARLDTMQAMLQRD
                     SLASEKWREAVAALRNNYEDIVARPDRGALYWPDFDWKGFDNQYHNWLTGMFRGDFGT
                     SYQDGRPVRNKIRDGLRWTLWLNIIAVLLSFGLSIPIGVWSAQRVGSRFDRSTSVLLF
                     MLYSLPVFWIATLLQVFFTTPEYGMDWFPTAGLSELPPTAPFWERLLDQTAHIFLPVV
                     CLTYGSLAFISRQMRGGMLQVLDQDYIRTARAKGLPERRVIWRHAFRNALFPIITLIG
                     SVFPSILAGSVVIEVIFGIPGMGKLTVDAIFARDWPVVFAVLMMASVLTVAGLLVSDM
                     LYAWADPRVRFQKKS"
     gene            complement(3664..>4054)
                     /locus_tag="PK198_18685"
     CDS             complement(3664..>4054)
                     /locus_tag="PK198_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40828.1"
                     /translation="KAGIHIELVAKDPLQIIDDIKKKNYDIAGGGRSASNTLWDPMQS
                     WHTAGDNRTGFGTPETDALIEEIQVTMDEKARNEKYKKLQEAIYEEQVEVYLFVPQDR
                     VLIHKRFEAEAHSVFPGFSVHRFRLKK"
     assembly_gap    4055..4197
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4198..5428)
                     /locus_tag="PK198_18690"
     CDS             complement(<4198..5428)
                     /locus_tag="PK198_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF40829.1"
                     /translation="MKTTAALPFFLFLVLITAFSGCKREPAVEYKRTDNTVIVGMPAD
                     ADRLNPLLATTAYARTVHEQIFSYLVAQDPVTGAFLPQLAKSLPERKETAEGQVAYTF
                     EIREEAKWDDGAPVTAADFIFTLKAILNPKVAAQRIRPYMAFIKKVETDAANPRRFTV
                     YSEKYILAEEAISAAFPVMQESHYDQAGLLKGIPLDQFLQPEVIAGLAETNENLGKFA
                     EAFSSEKYSREKGGVTGSGPYRLESWETGQRIALVKKENWWGEKSGKSNPAFAAYPDQ
                     ITFKILPDAVTTLNSIKAEEIDAVSNIDPKDYIDLENVASVSDRYVFASPLALSNLFI
                     YVNATHPILSDKLVRKALSHAVNAQEIIDNVFYGLGQPVNGPAHPSTNYYHKGLKPVE
                     FNVEKAKSLLTQAGWTDS"
     gene            5887..>6457
                     /locus_tag="PK198_18695"
     CDS             5887..>6457
                     /locus_tag="PK198_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF40830.1"
                     /translation="MASILLAGGAGLIGRRLRTLLAQEGHKVSILSRKANPYGDFPTF
                     VWDPEKGTIDHDAVRQADVVINLAGAGIADERWTPERKREIIESRTRSTRLLLESIQC
                     TGHSPQCFVSGAAIGYYGERGHEELSENATPGKGFLAESCIAWEQAIGEVADTGIRTV
                     ALRIGIVLSTRGGALEKMLLPARLGAGTYF"
BASE COUNT         1535 a         1746 c         1636 g         1397 t
ORIGIN      
        1 ctggcccgct ggaaggcgcg tcgatgaaca tggctcttcc cgccctcccc accggccatt
       61 gcccgtaaat ttcgccatac aggatgcgcg cttatcagga aaaacatctt tacatttgac
      121 cccgaaataa aatcatccat ccttttttct cacatcatca aaaatttagc gaaatgaagc
      181 aactgctacc tgcactcctt ctcctcctcc tgccggcgct gaccaacgcg caagctgtgc
      241 gccgcgtcat ggtggaatct tttacgcaag cctcctgcgg cccttgcgca tctcaaaacc
      301 cggccttcaa tgccctgctc caaagcaatg gagacaaggt ggtactgctc aagtaccaga
      361 caaactggcc tggctacgac cccatgaacg agcaaaaccc gtccgaagtg gccgctcgcg
      421 tgacatacta ccaaactaca agcgtaccca atgtgcgctt ggacggggta caaaacgccg
      481 gagtcgccag cgcagttact caggccatga tcaacaaccg cctcaatcag agcacgccca
      541 ttgacatgac gctggatcac cagatttccg ccgggtttga ttctatcttt attcagtgca
      601 aaatcaaaaa cctcggcgct acggagttca accccggcgg cgtggtgctg cacacggccg
      661 tcaccgaaaa gcagatcctt tttccgacag cccccggctc taacggcgaa gttgatttca
      721 aaagcgtgat gcgcaagatg ttgcccaatg cttccggcgc tgctttggcc gccattcccg
      781 ccggcgattc ggtgacgatc acttatgctg cggcactgcc ttcttacatt tacaatttcg
      841 cccggatcgg cgtggtggct tttgtgcaaa aaacctccga ccggcaggtg taccaggcag
      901 tagaaagctt tcccaagccc ttgcaaggca acatagtgga cgtggcagta tatcccaaca
      961 gcataggccc tgatggattt tgcaactacg ccctcacgcc gcaagtggat gtagccaatg
     1021 aaggcgacac ccccattaca tctatggatg tatcttacac cctcaacggc gccgccgcag
     1081 tgactcagga atgggaaggc acacttgctc ccggaggcac cgttaccatc acattcccgg
     1141 agattgccat tactcccggt gcagccgagg tggactatga tgtttccaat gccaatggag
     1201 atcattttga atataactca ttgaacgaat tgctgtcgtc ggaaatatac tacacactta
     1261 gcaatgaacc cgtaggtact cagatcacag aaggaatgga aagcactccc gtaagggcag
     1321 ttcctaccgg cactttggcg attaaagacg cagaggacca ttttatggta gtcaataaag
     1381 gagttttaag ctctagtgtt acccaagaag tgggaggcta cggcgcttct actaagtcta
     1441 tattctcaaa ttgctatggt tggaacgacc ccggcgccga agcgcacttg atctttgaaa
     1501 aaattgatct ttcgggatca accaacaatt atgtgaagtt tgattatgcc catgctcgat
     1561 accaaacgac cagcgaacgt atgcttgttt ccatttctac cgactgtggc gacacatgga
     1621 ctactatttg gaataaagcc ggcgctcaat tagcgactgc tgccgcaacc aacaactttt
     1681 atattcccgc cgtaactcat tggagaaccg actcagtggc gctcactaat ttcgacggta
     1741 acggagaggt attggtgcgt ttcaccgtag taactgccta tggcaataac atatacctcg
     1801 acaacatccg cgtatatcag ccggccactt ctgccgccga cgaacccggc ctgctcgccg
     1861 gcaaggtaca ggtgtatccc aacccggctt ctgatttggc tcgtatagac gtgcaactcg
     1921 cttctgcttc tgctgtcaac atctctgtgt tcgacctcag cggtaaattg gtgcacacca
     1981 tcgccaacgg caatctgctg agcgccggca atcaccagtt caactgggcg ccgcaagctc
     2041 agggcgtgtt cctcgtacgc gtagctaccg acttcggcgc tgtgaccgaa aaggtgacgg
     2101 tggtgaaata ataagcatgg agcatgcaag cgaagcgcag tcccgatgaa ggaacgaatc
     2161 gggagagcat agatacttgc tgtttgctcc ataaaaacaa aacaggctgt ccaacgatgt
     2221 ttgggcagcc tgttttttgt ttggcgggcg cgggcgcggt tctccccact caactcttct
     2281 tttgaaacct caccctggga tcagcccagg catacagcat gtcggaaacc aacagcccgg
     2341 ccaccgtgag tacagaagcc atcatcaaca cggcaaaaac caccggccag tcgcgggcaa
     2401 aaatggcatc caccgtgagc ttgcccatgc cgggtatgcc gaaaatgacc tcaatgacga
     2461 ccgagcctgc cagaatgctc ggaaatactg agccaatgag cgtaatgatg ggaaacaagg
     2521 cattgcgaaa agcgtgccgc cagatgaccc gccgctccgg cagccccttg gcccgcgccg
     2581 tgcgaatata atcctgatcg agtacctgca acatgccgcc ccgcatttgt ctggaaatga
     2641 aggccaaaga accataggtc aggcatacta ccggcaggaa aatatgggcc gtctgatcga
     2701 gtaaacgctc ccaaaacggc gcggtgggtg gcagctcgct cagcccggcg gtcggaaacc
     2761 agtccatgcc gtactccggc gttgtaaaaa acacctgcaa gagggtggct atccaaaaca
     2821 ccggcagcga gtacagcatg aacaacaaaa cagaggtact gcggtcgaac cggctgccga
     2881 cccgctgcgc cgaccaaacg ccgatgggaa tggacaggcc aaacgaaagc aacacggcga
     2941 tgatgttgag ccacagcgtc cagcgcaggc cgtcgcggat tttattgcgc accgggcggc
     3001 catcctgata cgaagtgccg aaatcgcccc gaaacatgcc ggtcaaccag ttatgatatt
     3061 gattatcaaa gcctttccaa tcaaaatcag gccaatagag cgcgccgcga tctgggcggg
     3121 ccactatgtc ttcgtaatta tttctaaggg cagcaaccgc ctctctccat ttttccgagg
     3181 ctaagctgtc gcgttgcagc atggcctgca tggtgtccaa tcgggcgcga atggccccat
     3241 cctggtgctg gagatacaga tcgcggagcg gcccggccaa cggcgacggc ccggcggcca
     3301 tgctgcgctc tgcctgctga atgctgtggt agtacgcctc aatgagcggc cagttgccat
     3361 attgcccgac gaggcgtttc agcgtttggc ggcgatcctg tcgtgcaatg agatgcagcg
     3421 tgtcggggta agctgccggg gcaatggaaa agtaaaaagc gggcttgtct aaacccatga
     3481 ggcgggcggt ttctgcatac aagcgatctg cctgagcggg gccggaggct atcggggccg
     3541 aacctgcgct gttgcgcaac tccaccgggt cgccgggcgc ggatttactc agcgcgaaag
     3601 cgagcagcga gatgagcaac agcgaaggca ggaaccacaa caatcgctga agcagatacc
     3661 ggatcatttt ttcagacgaa aacgatggac ggaaaagccc ggaaacacag agtgcgcctc
     3721 ggcttcaaag cgcttgtgaa tgagcacccg gtcttgaggc acaaacagat aaacttctac
     3781 ctgctcctca taaatggctt cctgtagttt tttgtacttt tcattgcggg ctttttcatc
     3841 catggtcacc tgaatttctt caatgagcgc atccgtttcc ggcgtgccga agcccgtgcg
     3901 attgtctccg gcagtgtgcc aggactgcat cggatcccac agcgtattgc tcgccgaacg
     3961 gccgccgccg gctatatcgt agtttttctt tttgatgtcg tcaatgatct ggagcgggtc
     4021 tttggctacc aattctatat gaatgccggc ttttnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     4201 aatcggtcca accggcttgt gtcagcagag attttgcctt ttcaacattg aattctaccg
     4261 gcttcaatcc tttgtgataa taattggtgg agggatgcgc cgggccgttc acaggctgcc
     4321 ccaatccata aaatacattg tcaatgattt cctgcgcgtt cacggcgtga gacagtgctt
     4381 tccggacgag cttgtcggaa aggatgggat gggtagcatt tacataaata aaaagattgc
     4441 tgagcgccaa aggtgaagca aacacatagc ggtcgctcac ggaagcaaca ttttccagat
     4501 caatataatc tttaggatca atattggaaa cggcatctat ctcctctgct ttgatggaat
     4561 tgagcgttgt gacggcatca ggcagtattt taaaggtaat ttgatcggga taagctgcaa
     4621 aggcggggtt ggatttacct gatttctcac cccaccagtt ttcttttttc accaatgcga
     4681 tgcgctgccc ggtttcccag gattccaggc gatacggtcc gctgccggta actccccctt
     4741 tttcgcgcga atatttctcg gatgaaaacg cttcggcgaa tttgcccaga ttttcattgg
     4801 tttcggctaa gccggcaatg acttccggct gtaaaaactg gtcaagaggt atgcctttca
     4861 acaggcccgc ctgatcgtaa tggctttcct gcatcactgg aaaagcagca gaaatggcct
     4921 cttctgccaa aatatatttt tcgctatata ccgtgaagcg gcgcggattg gcggcgtccg
     4981 tttccacttt tttgatgaac gccatgtagg ggcgaatgcg ctgagcggcc accttgggat
     5041 tgaggatggc cttgagcgtg aagataaaat cggcagcagt gaccggggcg ccatcatccc
     5101 atttggcctc ttcccgaatt tcaaaagtat aagccacctg gccctcagcc gtttctttac
     5161 gctccggcaa ggacttggcc aattgcggca aaaacgcacc tgtaacaggg tcttgcgcta
     5221 ctaaatagga aaaaatctgt tcgtgtacag tgcgggcata ggcagtcgtt gccagcagcg
     5281 ggttcaatcg gtcggcgtcg gcgggcatac caacaatcac cgtattgtcg gttcttttgt
     5341 actccacggc aggctcgcgc ttacagccgg aaaaagcagt aatcaatacc aaaaacagga
     5401 aaaagggcaa tgcagcagta gttttcataa gtttggaatg tgttgttggt gatagtgtta
     5461 tgagacaatc acacaaaagt aaagccaatt atctgaacgc cgtgaagtct gtaaagatat
     5521 ttgtttttgg gttcgaggtt tcgggttcaa ggttcgaggt tccgggtttc ctcacgctga
     5581 gcaccgccca cagagaactc cgcgccatgg cgcggctacc ggttccgggt tcaaggtttc
     5641 gggtttcaca aagcagataa actgcgaagc agatttcccc tccctctcca ttggcgatga
     5701 actgcgcacc gcgcaaggcg cggacagcag attactccaa agaagataaa ctgcgcagca
     5761 gatttcccct ccctctccat tggcgatgaa ctgcgaagca gcttactcca aagaagataa
     5821 actgcgaagc agatccccca tgaaccatgc ccctcgccca ttgtttcact cgcaaaccat
     5881 accaacatgg catccatttt actcgccggc ggcgccgggc ttatcggacg caggctgcgt
     5941 accctactcg cacaggaagg acacaaagtc tctattctca gcaggaaagc caatccttac
     6001 ggcgatttcc ccacttttgt ctgggacccc gaaaaaggca ccatagacca cgatgccgtc
     6061 cgccaggccg atgtcgtcat caatctggcc ggagcgggca ttgccgatga gcgatggacg
     6121 ccggagcgca aacgcgaaat catagaaagc cgcacgcgca gcacccgcct gctgttggag
     6181 agcatccaat gcacgggcca ttccccgcag tgttttgttt caggagccgc catcggctac
     6241 tatggcgagc gcggccatga ggaactgagt gaaaatgcca ctcccggcaa gggcttctta
     6301 gccgagagct gcattgcctg ggaacaggcc attggagagg tggccgatac cggtattcgc
     6361 acggtagcgc tccgcatcgg catcgtactg agtacgcggg gcggggcttt ggaaaaaatg
     6421 ttgctcccgg cccgtttggg agcaggtact tatttcg
//
LOCUS       DAORLF010000280         6436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6517_length_6436_cov_1.944993, whole genome shotgun sequence.
ACCESSION   DAORLF010000280 DAORLF010000000
VERSION     DAORLF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_6436_cov_1.944993"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PK198_18700"
     CDS             complement(<1..1340)
                     /locus_tag="PK198_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40831.1"
                     /translation="MKRPKQELDHFFRAAQLAQTQGIAPEICYGLCRQGKPADLKEYA
                     HEDVESLTRVLRKAVADNQIKADIQKLPEIAEAMVDKAIDLVYDKNHAFTRLVRMEGY
                     ASKQRLPEKKLSDFIRLYVKTKDKSPVFWSQLPEKLQISVEATENLKFTLQLNDITRA
                     HLPLITAIQRKGKRSLRSLVGFNWQALVDDKQIGVPSGIEGDTSPVQMATYIRMLEEA
                     VERAYPTAVFADYLKKNGNGLPGQADLNRFFKQFPDYDFRNSHIRRFVREQEPVIDQI
                     GLKDKDDCIARLFQLQRLFNITPETRKFETIPLLLQHRIDSAHNVKRMGRSAFVRDYV
                     LWRSDGPAEETQKVAREIYRRASRIAMGTLAVYGKYGQAFNNISMYGLPALTPPKTNG
                     QTIGISNLPNQSKAIVPEWEELFGALDYWQCSEGQSAYSPAAYLADLLIFLENAS"
     assembly_gap    1400..1421
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1422..2390)
                     /locus_tag="PK198_18705"
     CDS             complement(<1422..2390)
                     /locus_tag="PK198_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRF40832.1"
                     /translation="MQFSTKYLRPGQTNFQVWQLQQLLQQKGYAIQDSPGKFGETTAT
                     AIRAFQEAHQLPATGKIDAATAALLDKQWPEAVLSETAGFTIQGKLTHAENKQPLAGY
                     TVEIWEKDKQRDDLLGRLTTTDSGAYRLAFYGHHFMDNPQDFPPQVFFRVLDADGMLL
                     LNTREETQQLLPGKHTIHLSVDPEATDQAPLFRVYGRLTGDRATPAEGLLIRAYDRQL
                     GGQLYLLGEARTQADGSYSIWYNPEQLPAGKQQADLVLTVHHNPQEPAIFQSDMVADA
                     EPVQQFDIALPMGAYRGKSEYERITEGLKRQFPAFEFTSTLQPKTGA"
     gene            complement(2436..3224)
                     /locus_tag="PK198_18710"
     CDS             complement(2436..3224)
                     /locus_tag="PK198_18710"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40833.1"
                     /translation="MTTQQISEQVRQLILEGELDDAANALVQFFDKEWMSRGDKEQVQ
                     LYNQALYQLSQLNELKHQVFAGIISNEDADLKRNKIRAVLLEVSEALETFDPTRPQSP
                     APAPPSEEEGLRHKRRIAIILILGIPLSIMVVMLVFGVQMCNYVKNNILPTEEITQQQ
                     PITREQPVTPTAEDAFRGSRSDEPEMTGCFVRTRMMTGLLMEPEVMGQPFMELPDNTE
                     YKVLEVKKKTWARTTQYFFKIKHERYEGWVRQVELEFVSPECLR"
     gene            complement(3398..>3993)
                     /locus_tag="PK198_18715"
     CDS             complement(3398..>3993)
                     /locus_tag="PK198_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40834.1"
                     /translation="LDPQGKRILKNLQNDRKGIFRGIKIDIIEIPSGDEEGLKRVINR
                     LGNIRRVYFFGHGDVNWGPLFEYGPIALSFKANRFNANNVVFRAEQFTFNANALFLTC
                     NSFKYAMSFTEATGVSSIGIQGTTKYESNRLVAARLTDNSPNWDAIIWQFRRKIWEYI
                     PPIDNNWDSQSSIREVFFQLSREVVQNRTLWKRSGTN"
     assembly_gap    3994..4058
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4059..>6163)
                     /locus_tag="PK198_18720"
     CDS             complement(<4059..>6163)
                     /locus_tag="PK198_18720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF40835.1"
                     /translation="TAGQPLYAWDVNDHWENGEAIEERRIYHNTYDELRRPKEQYLRI
                     NDNPLQLLIEQTIYGEKGPDNAVGRNLRGQIWRHYDPGGLITNQRFDFKGNLLEIQRQ
                     MPSDYKANIVDWSMAQLETEVYTLITEYDALNRMSKLYNWHRDKTEAAVYEPKYSERG
                     VLLSEDLTIKGQKTTVVKDITYDAKGQRLRMRYGNGTTTCYHYDPQTFRLIQLRTTKY
                     NTDACERLAKSNLNDLNVYQNLFYTYDPVGNITEIYDDAYKPVFFKGQKVLPQNTYEY
                     DALYQLISATGREHYKLPAPTQRDFDWPPQQFDISDETLRGYTEQYQYDGVGNILKMQ
                     HIANGGSWTRNYQYATDSNRLIATEMGSSLSYPTYSDTPTLADHYTYDVHGSMTHIGN
                     ASANLITWDYRDMIQQYDLGGGGFAYYQYSSDKQRSRKVIEKQGNIKEERLYLGGMER
                     YRRWRNGDTNPEEEIETYHVFEGEQRILMVEDVIKTPPDGINKEGVLFCYQYSNHLGS
                     SCVEMDQDAEIISYEEYHPYGTTAYHSMNKDIQAAAKRYRYTGMERDEETGLAYHTAR
                     YYLAWLGRWGSCDPKFMVDGVNIFVYANNSPTKKMDIDGTNCNPENQSCPNPIIPTLR
                     EDLLQQSLQEGDREWNSNLELTVREELEQKSLPEGVRDWQGQQRVVFIGTSPQSGDHS
                     TPSSTHFTDVAIDFLTQEQ"
     assembly_gap    6164..6175
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6176..>6436)
                     /locus_tag="PK198_18725"
     CDS             complement(<6176..>6436)
                     /locus_tag="PK198_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40836.1"
                     /translation="EVLAIAHNYFRRPLNNHFEKTEEKYLTYTHLDTEGKALWIQDAR
                     GNIVMRYTLPAAAEADPLQDFCPAYDLAGNLLYQHSMDAGDRW"
BASE COUNT         1415 a         1507 c         1522 g         1893 t
ORIGIN      
        1 gaagcattct ccagaaaaat caataaatct gccaaatacg ctgccgggct gtatgctgat
       61 tgaccttcgg agcattgcca gtaatcaagg gcgccgaata attcttccca ttcgggtaca
      121 atggctttcg attggttagg caaattacta atgccaattg tctgcccatt tgttttgggt
      181 ggagtcaaag cagggagccc gtacatggaa atattgttga atgcctgccc atacttgcca
      241 taaaccgcca gggtacccat ggcgatacga ctggcccggc ggtaaatttc ccgggccacc
      301 ttttgggttt cctcagcagg cccgtcgctt cgccacaaaa cataatcgcg cacaaaagcg
      361 cttcggccca tacgcttgac gttgtgcgca gaatcaatgc gatgctgcag taaaagcggg
      421 atggtttcaa acttccgggt ttccggggtg atattaaaaa gtcgctgcaa ctggaacagt
      481 cgggctatgc aatcgtcttt gtccttcagg ccgatctggt ctatcacagg ctcctgttcc
      541 ctcacaaaac gccggatatg gctattgcga aaatcgtaat ccgggaactg tttgaaaaac
      601 ctgttcaggt cggcttggcc gggtaagccg ttaccgtttt tcttaaggta gtcggcaaag
      661 accgcggtcg ggtatgcacg ttctacggct tcctccagca tgcggatgta tgtagccatc
      721 tgcactggag acgtatctcc ctcaatcccg gatggcacgc cgatttgttt atcgtccacc
      781 aatgcttgcc agttgaaacc caccaggctg cgaagcgaac gtttgccttt tcgctgaatg
      841 gcggtaatca gcggcaggtg cgctcgtgtg atgtcattca gttgaagtgt gaatttcaga
      901 ttttcagttg cttcaacgga gatttgaagt ttctctggta attgactcca aaaaacgggc
      961 gatttatctt tcgttttaac gtacaagcga atgaaatcag acaatttttt ctccggcaag
     1021 cgctgtttgg aggcatatcc ttccatacgt acaaggcggg taaaagcatg attcttgtca
     1081 tacaccaaat caatcgcctt atccaccatt gcctccgcga tttccggcag cttctgtatg
     1141 tctgctttga tctgattgtc cgcaaccgcc ttgcgtagca cacgggtcag gctttctacg
     1201 tcttcgtggg cgtattcttt caaatctgcg ggtttgcctt gccggcacaa gccgtagcat
     1261 atttccggtg caatcccttg ggtttgggcc agttgtgcag cccgaaaaaa atgatcgagt
     1321 tcctgcttcg gacgtttcat cttgcgggac agaagttgta catcctgcga aagggggagc
     1381 gagagcggtt tgacaggcgn nnnnnnnnnn nnnnnnnnnn ncgcaccggt tttgggttga
     1441 agcgtagaag tgaattcgaa ggcggggaac tgccgcttca gaccttctgt gatgcgttcg
     1501 tattcggatt tgccgcggta tgcccccatg gggagggcga tgtcgaattg ctgcacaggt
     1561 tcggcatctg ccaccatgtc ggattgaaaa atagcaggtt cctgaggatt gtggtggacc
     1621 gtcagcacca gatcggcctg ctgtttgccg gcaggcagtt gctccggatt gtaccagatg
     1681 gagtatgagc catctgcctg tgtcctcgct tcgcccaaga ggtacagttg cccacccaat
     1741 tgtcggtcgt acgctctgat gagtaaacct tctgcaggag tagcgcgatc gccggtcaat
     1801 cgaccataaa cgcggaataa tggcgcctga tctgtggctt cgggatctac tgagagatgg
     1861 atcgtatgtt taccaggtag cagttgctga gtctcctcgc gggtattcag caacaacatg
     1921 ccgtcagcat caagcacccg aaagaaaact tgcggtggaa aatcctgtgg attgtccata
     1981 aagtgatggc cgtaaaacgc cagccggtag gcgccgctgt cggtagtggt gaggcgtccc
     2041 agcaagtcgt ctcgctgttt atccttttcc catatctcca ctgtatagcc agccaaaggc
     2101 tgtttgttct ctgcatgggt aagtttgcct tggatggtga atcccgccgt ttccgacagg
     2161 acagcctccg gccattgctt gtccaatagc gcagctgtag cggcatctat tttacccgtc
     2221 gcaggcagtt gatgggcttc ctgaaaagcc cggatggccg tcgcggtggt ttctccgaat
     2281 ttgccggggg aatcctgaat agcatagcct ttttgctgaa gcagttgctg cagttgccaa
     2341 acttgaaaat ttgtttggcc ggggcggaga tatttggtgg aaaattgcat gactgaatta
     2401 tttatcggtg aatggtgaaa ggttcatgtg gctggttacc tcaaacactc cggactcaca
     2461 aactccagct ccacctgcct cacccagcct tcgtagcgtt catgtttgat tttgaagaag
     2521 tactgggttg tcctcgccca agtcttcttt ttcacttcga gtactttgta ttcggtattg
     2581 tcgggcagtt ccataaaggg ctgccccatc acttcgggct ccatgagcag accagtcatc
     2641 atgcgggttc tgacgaaaca gccggtcatc tccggttcgt ccgagcggga gccgcggaaa
     2701 gcatcttcag cagtcggcgt cacaggttgt tctctggtta tcggctgttg ctgagtgatt
     2761 tcttctgtag gtagtatatt gttcttcaca taattacaca tttgtactcc gaaaaccagc
     2821 atcaccacca tgatgctcag tggaatgccc agtatcagga tgatggcgat acggcgcttg
     2881 tgacgcaatc cctcctcctc gcttggaggc gccggagccg gggattgggg ccgcgtggga
     2941 tcaaatgttt caagcgcctc actcacctcc agcaacaccg cccttatctt attccgcttc
     3001 agatccgcat cttcattgct gatgatgccc gcaaagacct gatgcttcag ttcgttcagt
     3061 tgcgagagct ggtagagcgc ctggttgtac agttgcacct gctccttgtc gccacggctc
     3121 atccattcct tgtcgaagaa ctgcaccaag gcattggcgg catcgtccag ctctccttcg
     3181 aggatgagtt ggcggacttg ttcggatatt tgttgtgtgg tcattgttta tttttatttt
     3241 ttcttattgc tttcgggcac tattgccgag tagtagagtc atgtgctgac ttgggggcgc
     3301 tgcgtgtaca cattgatttt tagttatttt tgacgaacac ttttgaacag cagggggctt
     3361 tttttagtaa agtcccacat agtggttata aattgcctta attagttcca gagcgtttcc
     3421 ataaagtgcg attttgtaca acctcacgtg aaagttgaaa aaaaacttct cttattgaac
     3481 tttgcgaatc ccaattatta tcaattggtg gtatatattc ccatattttc ctcctaaatt
     3541 gccatataat agcatcccaa ttaggtgaat tgtctgttaa acgagctgca accagtcgat
     3601 ttgattcgta ttttgtagtt ccttgtattc caattgaaga aactcccgta gcttcagtaa
     3661 aggacatggc atatttaaag gaattacaag ttaaaaacag tgcattcgca ttaaatgtaa
     3721 attgttctgc tctgaacacc acattgtttg cattaaacct atttgctttg aaagatagcg
     3781 caattgggcc atattcaaaa agtggtcccc aatttacatc cccgtgtccg aaaaaataaa
     3841 ctcttctaat attgcctaat ctattaatta ctctttttaa tccttcctca tctcctgaag
     3901 gtatctctat tatatctatt ttaatgcctc gaaaaattcc ttttctatca ttttgaagat
     3961 ttttcaagat tctttttcct tgtggatcta gttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tgttcttgag ttaaaaaatc
     4081 aatagcaacg tctgtaaaat gagtgctact aggggttgaa tggtctccag attggggaga
     4141 agttccgata aaaacaacac gttgttgccc ttgccaatct cttacacctt ctggtaagga
     4201 tttttgctct aattcctccc gaacagttag ttctaaatta ctattccatt ctctatcccc
     4261 ttcctgtaaa gattgttgaa gcaggtcttc ccttagagta gggataatag gatttgggca
     4321 agattgattt tctgggttgc aatttgtccc gtctatgtcc attttctttg ttggactatt
     4381 atttgcatat acaaaaatat ttactccatc aaccataaat ttcggatcac aacttcccca
     4441 cctacccaac cacgccaaat aataccttgc cgtatgatac gccaaccccg tctcctcatc
     4501 ccgctccatt cccgtatatc ggtaccgctt ggccgccgcc tgaatatcct tattcatgga
     4561 atgataagcc gtagtcccat acgggtggta ttcctcataa gatataatct ctgcatcttg
     4621 atccatctcc acacaggaag aacccaaatg gttgctgtat tggtagcaga aaagtactcc
     4681 ttccttgttt ataccgtcgg gaggcgtttt tatcacatct tctaccatca aaatgcgctg
     4741 ttcgccttca aaaacatgat atgtttcaat ttcttcttcc gggttggtat caccattgcg
     4801 ccaacggcga tagcgttcca tgccacccag gtataagcgt tcttctttga tgttgccttg
     4861 tttttctatc actttccggc tgcgctgttt gtcgctgctg tattgataat aagcaaatcc
     4921 accaccgccc aaatcgtatt gttggatcat atctcggtaa tcccaggtaa tgaggttggc
     4981 acttgcattg ccgatatgcg tcatgctgcc atgtacatca taagtgtaat ggtctgccaa
     5041 agtgggcgta tcgctgtaag tggggtagct taagctgctg cccatttcgg tcgcaatgag
     5101 gcgattgcta tcggtagcat attgatagtt acgcgtccac gagccgccat tggcaatatg
     5161 ctgcattttt aaaatattac ctactccgtc atattggtat tgctcggtat atccgcgcag
     5221 ggtttcatca ctgatatcaa attgttgtgg cggccaatcg aaatcgcgct gtgttggtgc
     5281 aggcagctta taatgttctc tgcctgttgc gctaatcaat tggtaaaggg catcgtattc
     5341 gtaagtgttt tgcggcaaca ctttttgtcc tttgaaaaat acgggtttgt acgcatcgtc
     5401 gtagatttca gtgatattgc caaccgggtc gtaggtgtaa aataaatttt ggtaaacatt
     5461 gaggtcattc aaattgcttt ttgccaggcg ttcacaagcg tctgtgttgt atttggttgt
     5521 tctcaattgt atcagcctga atgtttgtgg atcgtaatgg taacaggttg ttgtgccatt
     5581 gccgtagcgc atacgcaggc gttgtccctt ggcatcatac gtaatgtctt tgacaacagt
     5641 ggttttctga cccttgatgg tcaaatcttc acttaacaac acgccccttt cgctgtattt
     5701 aggctcataa acggcggctt cagttttatc ccgatgccaa ttgtacaact tgctcatgcg
     5761 attgagcgca tcgtattcgg ttatcagtgt ataaacttcc gtttccaatt gagccatgct
     5821 ccagtccacg atattggcct tgtaatccga aggcatttgc ctttgtattt ccagcaggtt
     5881 acctttaaaa tcaaaacgtt ggttggtgat caggccgccg gggtcgtagt gccgccagat
     5941 ttgcccgcgc aggtttcttc cgacggcatt gtccgggcct ttttccccgt atatggtttg
     6001 ttcgatgagt agttgcaagg ggttgtcgtt tatgcgcagg tattgttcct ttggtcggcg
     6061 caactcgtcg taagtgttgt ggtaaatgcg ccgctcctct atggcttcgc cattctccca
     6121 atggtcgttc acatcccaag cgtagagggg ttgccctgcc gtgnnnnnnn nnnnnccagc
     6181 ggtcgccggc gtccatgctg tgttggtaga gcaggttgcc tgccagatcg taggcagggc
     6241 aaaaatcttg cagcggatcg gcttctgctg cagcgggtaa agtgtagcgc atgacgatat
     6301 tgcctcgcgc atcctgtatc cagagggctt tgccttcggt gtcgaggtgg gtgtaggtca
     6361 agtatttttc ttcggttttt tcaaagtgat tgttcaacgg tcggcggaag tagttgtggg
     6421 cgatggccag tacttc
//
LOCUS       DAORLF010000281         6398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6562_length_6398_cov_1.770298, whole genome shotgun sequence.
ACCESSION   DAORLF010000281 DAORLF010000000
VERSION     DAORLF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_6398_cov_1.770298"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..126
                     /locus_tag="PK198_18730"
     CDS             7..126
                     /locus_tag="PK198_18730"
                     /inference="COORDINATES: protein motif:HMM:NF017315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRF40837.1"
                     /translation="MTKGSGTVLIGGRPAARIGDLTAHGGIIIAGAPNVLIGG"
     gene            179..601
                     /locus_tag="PK198_18735"
     CDS             179..601
                     /locus_tag="PK198_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HRF40838.1"
                     /translation="MTQEQSFLGTGWSFPPWFSPDTGTVEMVSAETDINQSIQILLST
                     SLGERVMQPKYGCNLSDYQFEPVNSNLLAFLKDMVENALLYHEPRIVVEEVTITQPGS
                     FDLIEGRLTIEVDYRIIETNSRYNYVYDFYLREADRNI"
     gene            640..>2344
                     /locus_tag="PK198_18740"
     CDS             640..>2344
                     /locus_tag="PK198_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40839.1"
                     /translation="MNLCELKHPLREEPGSSQLKRANDVLQAATVPIDGRTWADILDY
                     LYRYSRQINFTDVAQSEESGAFTRSGDWRVFLENSLPFLLARISKFDGEAIDKKYARL
                     QSDFERMPGPDTFSSLLDFCFEELIEPAFNWYETLSREERLQKEAAGTLPEAAISEFR
                     FKRLLANTIGADLKLPLLDFIKIYQKAQRAQAVTQRNFFPFVNQQIWGLKVTDLLKPV
                     DPTSAFDRTESARLLADEVFGKFLNILRQISRSAPDYIAESLKPLQEDFRKRHDPHLA
                     LLFTFLELFQYVQGDMNRLSEKHLDFFYRQVLRIQPKPHRPDHAHLVFEVAKHLEQYL
                     LKKDTVFKDGKDNNKADVLFSLDKEIVLDKAAIGSLRTLYLNQVKLPAKPKSATTVND
                     CVSQEVIALEGVYMAPVANSADGLGQAFPENQIPNWATLGAKESKTLAPGKDKLDHHP
                     NARIGLMLASPVLLLQEGKREIHIVLNCANVLNLDDDTLNAYINSVNKALRKPYYVLT
                     ERVLQQLANAFSFNALALLKCHLQDDEVIDFDSLCRKYSGQYSEKEKLILKCYLDDTA
                     SD"
     assembly_gap    2345..2354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2355..>2657
                     /locus_tag="PK198_18745"
     CDS             <2355..>2657
                     /locus_tag="PK198_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40840.1"
                     /translation="KSKIEVLKGVLLEYGNWETASQQSPVLKNTNDRLQNPLSDEGRA
                     FLTGLLSGKNPYPLGYDLLNCLNVKNEPSCEWLFSCCEIKLLECFLEREERVMCCLT"
     assembly_gap    2658..2723
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2724..>3988
                     /locus_tag="PK198_18750"
     CDS             <2724..>3988
                     /locus_tag="PK198_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40841.1"
                     /translation="VFYRNDLKECLPIFIDTDELATFVGENSELLFSEADLETLKKHL
                     ACNNIFKIALTGEKGWYVPKRIKTKISPLEGLDIKDCEGNTTSKLQNIQFDIKIVLDE
                     TEPAIICYDLEKHKEDYQFEKNFPVAKIELNSELKLEVPDDSHNPCDPLDIEEACKKS
                     RTKPLITPGDCCCLERRGESAGKSYDLYQFLRRLQVADAGIDVKVCGIKKNIVIQNDE
                     NLLDPNSPFQPFGVRPVVVDADKKKDGDTTPAPTPPVGGCPSPPIIPNANLSGPCFYI
                     GSNEILKKCWKDLCIHINWKDKPGNLEEYYAAYDPELKTSDFEVNISVLDGGHWHGEG
                     TSARPKEKTCCPEEGSNTPDNPELKGQNPCTLHYNRKLFESKGDGCACKCPKDLPPYP
                     SPFPFEQTIKIDSRYFPLDKNKGKDVCRP"
     assembly_gap    3989..4122
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4123..>4709
                     /locus_tag="PK198_18755"
     CDS             <4123..>4709
                     /locus_tag="PK198_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40842.1"
                     /translation="VVPKAVFQIPGLAALLGDDQFTFEELLVAIKTYVLDCTTEILGE
                     SNSTNANEICGLFNTFMSGISYWNFFTGPGQDDADITTIGEVRGDLINLENAVKNLID
                     PKLLDKIKVFFKDIQDAFDKGNAILIPNEPWTPTIKNICLDYSACAGKDDIDIVHLYP
                     FEGTYKHEDLEANPTLLPVFEDEGTLYIGLSNLRPG"
     assembly_gap    4710..4734
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4735..>5273
                     /locus_tag="PK198_18760"
     CDS             <4735..>5273
                     /locus_tag="PK198_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40843.1"
                     /translation="QLAEATADSERDRALVRWYHLANNQWQELRPGFDIISDETKGLT
                     VSGIVKIALPKGITRTGNTVMPPDLFWIKASVSEVDDAEQCEIPGDAVSLQPIQKVKA
                     ISEAIAVHTQAARVTFKPESANDLQRLARELPAGSITKMAEPEAGVKKTAQPYASFHG
                     LPPESGNSFYKRVSEHLRH"
     assembly_gap    5274..5404
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5405..6019
                     /locus_tag="PK198_18765"
     CDS             <5405..6019
                     /locus_tag="PK198_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40844.1"
                     /translation="LDWAPGFVSIAVVPDLTKLKPGQDLEPRAPVSLLEEIKAYLKTK
                     ISPFVRLKIMNPRYEKVNLNVRVRLRKGKDKTFYAARLKEDLTNLLAPWYITRDSDKL
                     VFGQAVSESEVIRYIESLDYVDFITCLSLSGDEDAPSLLRAIGPVTARSILTAGEICV
                     VVDEDDCARYSDTQPDCFAEAGNEFPFHQICKTPAPPPEEEYGP"
     gene            6079..>6398
                     /locus_tag="PK198_18770"
     CDS             6079..>6398
                     /locus_tag="PK198_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40845.1"
                     /translation="MSTNTLHSKVIRQVDESWPAHLDFARLLEEGLRHIGDLAGRIWT
                     DHNTHDPGITTLEVLCYALLDLGYRTQLPIEDLLAQKDGAAEDNFFTPAQILSCNPLT
                     ITDYR"
BASE COUNT         1658 a         1581 c         1440 g         1353 t
ORIGIN      
        1 gatgtaatca ccaaaggctc cggaacagtc ttgataggcg gcagacccgc cgctcgaatc
       61 ggagacctta ccgcacatgg aggaatcatt attgcagggg cgcccaacgt cttgatcgga
      121 gggtagtatt ccttcgaagc agatatgccc aagcccatcc acaaatacca acacagctat
      181 gacacaagag caatcattcc tcggaacagg ctggtcattt cctccgtggt tttctcccga
      241 taccggcaca gtggaaatgg taagcgcaga gacggacatc aatcaaagca ttcaaatcct
      301 attatccacc agtttgggag agcgggtcat gcagcccaaa tacggctgca acctgtctga
      361 ctaccagttt gagccagtga acagcaatct gctggctttt ttaaaagaca tggtggaaaa
      421 tgccttgctt taccacgagc cgcgcatcgt tgtggaagag gtgaccatca cacaaccggg
      481 gtctttcgac ctcattgagg gacgcctcac cattgaggta gattacagga ttatagagac
      541 gaattcccgc tacaattatg tgtatgattt ctacctgcgc gaagccgaca ggaatatctg
      601 accacggcaa catttctccc aaatcttcac cgcatcataa tgaacctctg cgaacttaaa
      661 catccgctaa gggaagaacc ggggtcgagc caactcaagc gcgccaacga cgtgctgcaa
      721 gcggctaccg tacccataga tgggcgcacc tgggccgata ttctggatta tctctaccgg
      781 tactcccggc agataaactt caccgacgtt gcgcaaagcg aggaatctgg tgcatttacg
      841 cgatcgggag actggcgcgt atttctggaa aacagcctcc ctttcctgtt ggcgcgcatc
      901 agtaaattcg atggagaggc cattgacaaa aagtatgccc gtctgcaatc cgatttcgag
      961 cgcatgcccg gcccggatac gttcagttct ctgctggact tttgctttga agaactcatt
     1021 gagcctgctt tcaactggta tgaaaccctg agcagagaag agcgcctcca aaaagaggcc
     1081 gcaggtacgc tcccagaggc ggccatttct gagttcaggt ttaaacgctt gctggccaat
     1141 acaatcggcg ccgacttgaa acttccgctg ctcgatttta ttaaaattta tcaaaaagcg
     1201 caaagggctc aggcggtgac acagcgaaat ttttttccct ttgtcaacca gcaaatatgg
     1261 ggattaaagg tgaccgacct attgaagccg gtggatccca cctccgcttt cgaccggaca
     1321 gaatccgcca gactactggc ggacgaggtc ttcgggaagt ttctcaatat tttgcgccaa
     1381 atcagccgtt cagcgcctga ctatattgcc gagagcctga agcccttgca ggaagatttt
     1441 cgcaaacggc acgacccgca cctggcactg ctgtttactt ttctggagtt atttcagtat
     1501 gtgcagggcg acatgaaccg cctgagtgaa aaacatctcg attttttcta ccggcaggtg
     1561 ttgcgcattc agcccaaacc gcaccgcccg gaccacgcgc acctggtctt tgaagtagct
     1621 aaacacctgg aacagtacct gctaaaaaag gacactgtat tcaaagacgg caaggacaac
     1681 aacaaggccg acgtactctt ctcccttgac aaggagatcg tactcgacaa agcagccata
     1741 ggcagcctgc gcaccctgta ccttaatcag gtaaagttgc ccgcaaaacc caaaagcgca
     1801 acaacagtaa acgattgcgt atcccaagag gtcatcgcgc tggagggggt gtatatggcg
     1861 ccggtggcca actcggccga cgggcttggg caggctttcc cggagaacca aattcccaat
     1921 tgggctacct tgggcgccaa agaatccaaa acgctcgcac ccggtaaaga caaactcgat
     1981 catcatccca acgccagaat agggcttatg ctggcttctc cggtgctgtt gctgcaggag
     2041 ggtaagcggg aaattcacat cgtgctgaac tgtgccaacg tcctgaattt ggacgatgat
     2101 acgttgaatg cctatattaa tagtgtaaac aaggcactgc gaaaacctta ctatgtcctc
     2161 accgaaaggg ttttgcaaca attggccaat gcattctcct tcaatgccct ggccttgttg
     2221 aaatgtcatc tgcaggatga tgaggttatt gactttgaca gcctgtgcag aaaatacagc
     2281 ggacaatatt cggagaagga gaagctgatc ctgaaatgtt acttagacga tactgcctct
     2341 gatgnnnnnn nnnncaaatc taaaattgaa gtattgaaag gtgttcttct tgaatacgga
     2401 aactgggaaa ctgcctccca acaatctcct gttttgaaaa ataccaatga ccgactccag
     2461 aaccctcttt ccgatgaagg gcgagccttc cttaccggcc tgctttctgg aaaaaaccct
     2521 tacccgctcg gttacgactt gctcaattgc ctgaacgtca agaatgagcc ctcctgcgaa
     2581 tggctgtttt cctgttgcga gatcaagttg ttggaatgtt ttctggagcg ggaagaaagg
     2641 gtgatgtgct gtctgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnggtattt taccgaaacg atctgaaaga atgcctgcct
     2761 atattcattg acacggatga actggccact tttgtcggcg aaaatagtga actgcttttt
     2821 tccgaagccg atttggagac gttgaaaaaa caccttgcgt gcaacaacat tttcaaaatt
     2881 gccctgacag gtgaaaaggg ctggtatgta cccaagagga tcaaaacgaa aatatcaccg
     2941 ttggagggct tggatataaa agactgcgaa ggaaatacaa cctctaaact tcaaaacatc
     3001 cagttcgaca ttaaaatagt ccttgacgaa acagaaccgg ctattatctg ctacgacttg
     3061 gaaaaacaca aggaggacta ccaatttgaa aaaaactttc cggtagcaaa aatagaactg
     3121 aacagcgagc tgaagctcga agtgccggac gacagccaca acccttgtga tccgctcgat
     3181 atagaagaag cctgcaaaaa atcgagaaca aagccattaa tcacacctgg tgattgttgc
     3241 tgcttagagc gaagaggcga atccgccggt aagtcctacg atctttacca attcctgcgc
     3301 cgtctgcagg tggcggatgc cggtattgat gtgaaagttt gcggtatcaa aaagaacatt
     3361 gtcatccaaa atgatgaaaa cctgctcgac cccaacagcc cgtttcagcc tttcggggtg
     3421 aggccggtgg tggtggacgc agataagaag aaggacggag atacaactcc tgcacctacc
     3481 ccccctgttg gtggttgccc aagccccccc ataataccta atgccaatct ttcaggccca
     3541 tgtttttata tcggcagcaa cgagattctt aaaaaatgtt ggaaggactt gtgtatccac
     3601 atcaactgga aagacaagcc cggcaacttg gaggagtact acgccgccta tgatcccgag
     3661 ttgaaaacat cagacttcga ggtaaatatc tctgttcttg atggtgggca ctggcatgga
     3721 gaaggcactt cagcacggcc aaaagaaaag acctgctgcc cagaggaggg ttctaataca
     3781 cctgataacc cggagttaaa aggacaaaac ccatgtacgc ttcactacaa ccggaagttg
     3841 tttgaaagta aaggcgatgg ctgcgcctgc aaatgcccca aagatttgcc gccctaccct
     3901 tctcctttcc cctttgaaca gaccataaag atagatagcc ggtattttcc tttagacaag
     3961 aataaaggaa aagatgtgtg caggccagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtagttcc caaggcagtc
     4141 tttcagatac cgggccttgc cgcgctgttg ggtgatgacc aatttacttt tgaagaactg
     4201 cttgtcgcta ttaaaactta tgttttggat tgtaccacag agatattagg agaaagcaat
     4261 tcgacgaatg ccaatgaaat ttgtggttta ttcaatacct tcatgtcagg catttcctat
     4321 tggaattttt ttactggccc aggacaagat gatgctgata ttacaactat tggagaggtc
     4381 aggggtgatt taatcaatct tgaaaatgca gtaaaaaacc ttattgaccc aaaactttta
     4441 gataaaatca aggtcttttt caaagatatt caagatgcct ttgacaaggg gaatgccatt
     4501 ctcatcccca acgaaccctg gacccccacc atcaaaaaca tctgcttaga ctactccgcc
     4561 tgtgccggaa aagacgacat tgacattgta cacctctatc ccttcgaggg cacatataag
     4621 catgaagacc tggaagccaa ccccacactg ctgcctgttt ttgaagacga aggcacgctg
     4681 tatatcggct tgagcaactt acggccgggn nnnnnnnnnn nnnnnnnnnn nnnnccaatt
     4741 ggcagaggcc accgcagaca gcgagcgcga ccgggctttg gtcaggtggt accacttggc
     4801 caacaaccaa tggcaggaat tgaggccggg cttcgacatc atttccgatg aaaccaaggg
     4861 gcttaccgtt tcaggtatcg taaaaattgc cctgccaaag ggcattaccc gcaccgggaa
     4921 cacggtgatg ccccccgacc tgttttggat taaagcttcg gtaagcgaag tggacgatgc
     4981 cgagcaatgc gaaatccccg gcgacgccgt ttctctacag cccatacaaa aagtaaaagc
     5041 cataagtgaa gccattgccg tacatacgca agccgcaagg gtcactttca aaccggaatc
     5101 ggccaacgac ctgcagcgcc tcgcgcggga actcccggcg ggaagcatta caaaaatggc
     5161 cgagccggaa gccggcgtaa aaaaaaccgc tcagccgtac gcctccttcc acggcttacc
     5221 gcccgaatcc gggaacagtt tttacaagag ggtaagcgaa cacttgcggc acannnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnttggac tgggcgcccg gcttcgtttc catcgcggta gtacccgatc tcaccaagct
     5461 gaaacctggc caagacctcg aaccacgcgc acctgtcagc ctgctcgaag aaataaaagc
     5521 ctacctcaaa acaaagattt cgccctttgt caggcttaaa ataatgaacc cccgctatga
     5581 aaaagtgaac ctcaacgtgc gggtgcggct gcgcaaagga aaagacaaaa ccttctacgc
     5641 tgctcgtttg aaggaagacc tgaccaatct tttggcgcct tggtatatta cccgcgactc
     5701 cgacaagctg gtctttgggc aagctgtcag cgaatcggaa gtgatccgat acatcgaaag
     5761 cctggactat gtggacttca tcacttgcct atcgctgtca ggcgatgaag atgcccccag
     5821 cctgctcagg gcaatcggcc cggtgactgc ccgctccatc ctcactgccg gggagatatg
     5881 cgttgtcgtg gatgaggatg attgcgccag gtattcggat acgcaacccg attgctttgc
     5941 tgaagccggc aatgagtttc cttttcacca gatttgtaaa acgcctgctc ccccaccaga
     6001 agaggagtac ggcccctgaa aaatgcctcc ctgggcaatg tgtctcaata ccgaatgact
     6061 acattccatc attaaaccat gtcaacaaac acgctccata gcaaggtaat tcgtcaagtg
     6121 gacgaatcct ggccggccca tttggatttc gcccggctcc tggaagaagg tctgcggcat
     6181 atcggcgacc tggccggccg tatctggacg gaccacaaca cccacgatcc cggcatcacc
     6241 accctggaag tgctttgtta tgccctgctg gacctggggt accgcaccca actgcccata
     6301 gaagacctgc tggctcaaaa agacggcgct gccgaagaca atttcttcac gcccgcccag
     6361 atactctctt gcaatcccct caccatcacg gattaccg
//
LOCUS       DAORLF010000282         6388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6577_length_6388_cov_1.694300, whole genome shotgun sequence.
ACCESSION   DAORLF010000282 DAORLF010000000
VERSION     DAORLF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_6388_cov_1.694300"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    371..594
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<595..1622)
                     /gene="gcvT"
                     /locus_tag="PK198_18775"
     CDS             complement(<595..1622)
                     /gene="gcvT"
                     /locus_tag="PK198_18775"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRF40846.1"
                     /translation="MKNTPLTDIHLALGARMAEFAGYNMPISYAGIQDEHHAVRKGVG
                     MFDVSHMGEFIVRGRQALDLVQYVTSNDASQLSIGEVQYSCLPNGRGGIVDDLLVYRL
                     PEEMCSEGEQAFMLVVNASNMEKDWNWISGHNRFDTRLINISEQTALMAVQGPKAAEA
                     LQSLTDIDLSAMKYYTFAKGRFAGLDNVLVSATGYTGAGGFEIYVAAEHAVQIWNAIM
                     EAGQAFDIKPIGLGARDTLRLEMGYCLYGNDIDDNTSPLEAGLGWITKLQKGDFIDAE
                     FLRKQKAAGVQRKLVGFKVEDRRVPRHGYVIEDAEGNVVGEVTSGTQSPTLGYPVGMG
                     YVPTGMAA"
     assembly_gap    1778..1939
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1940..>2156)
                     /locus_tag="PK198_18780"
     CDS             complement(<1940..>2156)
                     /locus_tag="PK198_18780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF40847.1"
                     /translation="TIDFFAGKPESPARKPDYSHPIDWKVLDEFEIEPTAIIRTTKGN
                     IRIRLLPTVAPGSVVNFIELARDKFYDG"
     assembly_gap    2157..2166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2167..3697)
                     /locus_tag="PK198_18785"
     CDS             complement(<2167..3697)
                     /locus_tag="PK198_18785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRF40848.1"
                     /translation="MLRTAFWSALFFLLALAACRPYQKDDTFTDVDIDLKDPVFRQIY
                     DLQDRRQSDSLYQWLANDNPNYRYAAALAFASIKDTAAVPKLTELLADPVDEVRAAVA
                     YAIGQTGAAAGEAPLIAAFDQTDTLGAYRKSNRAILEAVGKCGSLNTLRQLSTVSTYL
                     PSDTALLEGLTRGIYRFALRNIIADEGTARMIQIAAGTEYPPSARLVAANYLSRAKDL
                     VIDSTAFMPVVKITGSETDPAIRMALVMGLGKAKNQAALATLLEMLPKEQDYRVKVNI
                     LRALGSYDYAAVQSAVILALKDPNPHVAARAAQFFVENGQAQDAAFYWRSSKDSIHWS
                     AQLQLYTAAQRHLPSYQAETRNIINWELRQRFSGAASAYQKAVALRALAEFPWNMRYI
                     QRESFASPDAAVRTAGVESLDGIARRADFAKVFGANARRTSIELALYFKQAIQSGDPG
                     MMAAAASALRNPDRNFKAALDSVGTSFLDSALVKLPLPREIETYNEVQRIHIDAKKRM
                     QD"
     gene            complement(3723..>4528)
                     /locus_tag="PK198_18790"
     CDS             complement(3723..>4528)
                     /locus_tag="PK198_18790"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF40849.1"
                     /translation="TPANASGGYSGKTFNLRDGLRNSVNTVSVHLVKEIGDVEPIRGL
                     VHMMGIDSTTRYPNGRHRVPKSPAICLGATDLSVMEMTGAYTTFANNGMYVKPVFVTR
                     IEDRNGRVIYSAMPEEKQALSPSANYAMLDLLKYSATNMGGIKSEVGGKTGTTNDYVD
                     GWFMGVTPRLVVGTWVGGEDRWIRFLSIANGQGSYMSKPFFSAFIKALEEAPGIDYDA
                     SVRFARPPGDMDIELDCSKYSSGRPAFKDLDDEFSNDIFGDEKPEGEDE"
     assembly_gap    4529..4538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4539..6298)
                     /locus_tag="PK198_18795"
     CDS             complement(<4539..6298)
                     /locus_tag="PK198_18795"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRF40850.1"
                     /translation="MWQTITDFLFQTERSSYRNWAVWMWRLLFIGIGAVVLIFVLLSF
                     TNLPSVSQLENPKSELASVVYGSSGTEIGRYYIENRVPVGYEDLSPHLVNALIATEDE
                     RYYSHTGIDFQALGRVAVKTIALQKKGSGGASTITQQLAKLLFTGQKAEGWGRVLQKL
                     REWIIAVRLERSYTKEEILAMYLNKFNFINGAYGIKAASEIYFDRPQDSLEVQEAAML
                     IGMLKNPSLFNPLRRPEQVQQRRNVVFLQMYKNGLLRKDELESLKKLPLGLRFTRQTH
                     IDGLAPYFRMELGKYVKEEILAKEQYYKPDGTPYDIYRDGLKIYTTIDPQMQKIAEEV
                     MARHMAKVQDAFFKTWNKRDPWKYSQGSETEIPVAVRAQRLKGLVRASERYQALRDKY
                     LLEVAEKVRAEMGDDKLLNDDWEVELVMAEAARPDSLARLVKAKKLSEETARRLKNVL
                     RSSHLSQLQTAWSKLQDASDREFSTPVAMRVFAYNEEMEKDTVMSPLDSIRYHRMFLQ
                     TGILAVDPATGYVKVWVGGINHKYFQYDHATINRQVGSTFKPFVYATAIEIYRFSPCY
                     TVADVAKTISPGEGNFGLFQS"
BASE COUNT         1307 a         1532 c         1705 g         1438 t
ORIGIN      
        1 actctgtgca gaactgcaat gcctcctaaa tatcggcact acgtctgcac tatcgttctg
       61 cgataaaaaa tgaaaaataa caagatgtat aaagttgaat acttaattgt gaggtactcc
      121 ccattcattg gacaaaaaaa cgggttcaat aaggtgagag ccgacgcatg gaaacaatcc
      181 tgatcggaac aaagaacagc ccgttgggct gctctccgtc ctgatcctat tttgttggca
      241 ggacaaagtt agccccacgt tttgacattt caaagttgaa tggaaggaac ttttaccttc
      301 ccgaaaaaaa catcggcggg agtatgccca ttgagatggg ggttaggccg ttggttgttg
      361 tagaactcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggca
      601 gccatccctg tcggcacata gcccatccca acggggtagc ccagcgtcgg tgattgcgtg
      661 ccggaagtga cctcgcccac gacattgccc tccgcatctt ctatcacata gccatgacgg
      721 ggtacacggc ggtcttccac cttgaagccc acgagtttgc gctgaacgcc tgctgctttt
      781 tgcttgcgca gaaattctgc atctataaaa tcgccttttt gcagcttggt aatccaacct
      841 aaaccggcct ccaagggcga ggtgttgtcg tctatatcgt tgccgtacag gcagtaaccc
      901 atttctaaac gcagggtatc gcgcgcgccc aagccgatgg gtttgatgtc aaaagcctga
      961 cctgcctcca tgatggcgtt ccagatttgt acggcgtgtt cggcggccac atatatttca
     1021 aaaccgcccg cgccggtgta gcccgtagcc gataccagca cgttgtccaa tccggcgaaa
     1081 cggcccttgg caaaggtgta gtatttcatg gccgacaagt ctatgtcggt caacgattgc
     1141 agggcttccg cagctttggg accttgcaca gccatcagtg ccgtttgttc cgaaatattg
     1201 atgaggcggg tgtcgaaacg gttgtggccg ctgatccagt tccagtcttt ttccatgttg
     1261 gaggcattga cgaccagcat gaaggcttgc tctccctcgg aacacatttc ttccggcagg
     1321 cgatacacca gcaggtcgtc cacgataccg ccgcgaccgt tgggcaggca agaatattgc
     1381 acctcgccga tggagagttg ggaggcatcg ttggaggtga cgtattgcac cagatcgagc
     1441 gcctgcctgc cgcgcacaat gaactcgccc atgtgcgaaa catcgaacat gcccacgccc
     1501 ttgcgcaccg cgtggtgttc gtcctggatg cctgcgtaag aaatgggcat gttgtagccg
     1561 gcaaattcgg ccatgcgggc gccgagcgcg aggtgaatat cggtaagcgg agtatttttc
     1621 atagttagga attattgaat gataatactt tcaattttgc tgccgggctg aatctgatgc
     1681 accacatcca tgccgtctat gacgcgggcg aaaatggtgt agttgccatc cagatgcagg
     1741 gccggcgaat gggtgatgaa aaactgtgtg ccttctgnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnng ccgtcgtaga atttatcgcg ggccaattct atgaagttca
     1981 ccaccgaacc gggcgccacg gtgggcagca gccggatgcg gatattgcct ttggtggttc
     2041 ggatgatggc ggtgggttct atttcaaact cgtctaacac cttccaatca atggggtgcg
     2101 aataatcggg tttgcgggcc ggcgattccg gtttgcctgc aaagaaatca atggtgnnnn
     2161 nnnnnncatc ctgcatccgc ttcttcgcgt cgatatggat gcgctgtact tcgttgtagg
     2221 tctcgatctc tctgggcaga ggcagtttca ccaatgccga atcgaggaaa cttgtgccga
     2281 cggaatcgag tgcagccttg aagttgcggt caggattgcg cagggccgaa gcggcggcgg
     2341 ccatcatgcc gggatcgccc gattgaatgg cctgtttgaa atacagcgcc agttcgatgg
     2401 aggtacggcg ggcattggct ccgaatactt tggcaaaatc ggcgcggcgg gctatcccgt
     2461 ccaaagattc cacaccggca gtacgcacgg cggcatcggg cgaagcgaaa ctctcgcgct
     2521 gtatgtagcg catgttccag ggaaactcgg ccaaagcgcg cagcgccact gctttctgat
     2581 aggcgctggc ggccccgctg aaacgctgcc ggagttccca gttgatgatg ttgcgggttt
     2641 ctgcctgata agagggcagg tgtctctgag cggcagtgta aagttgcaat tgggcgctcc
     2701 agtggatgga gtctttagag ctgcgccagt aaaatgcggc atcctgggcc tgtccgtttt
     2761 ctacaaaaaa ctgggctgcc cgcgccgcca catgcggatt ggggtctttg agcgccagaa
     2821 tgaccgccga ctgcacggct gcatagtcat agctgcccag cgcgcgcaag atgttgactt
     2881 tcacgcgata gtcctgctct ttgggcagca tttcgaggag ggtggccaat gccgcctgat
     2941 ttttggcttt gcccaatccc atcactaaag ccatgcggat ggcagggtcg gtttcggagc
     3001 ctgtaatttt gacgacgggc ataaacgcgg tactgtcaat gaccaaatct ttggctcggc
     3061 tgaggtagtt ggcagctacc aaacgggcgc tcggcgggta ttctgtgccg gcggcaatct
     3121 gaatcatgcg ggcggtgcct tcatcggcaa tgatgttgcg caaagcaaag cgatagatac
     3181 cgcgcgtaag gccttccagc agggcggtat ccgagggcag ataggtgctc acagtactga
     3241 gttggcgcag ggtattgagg ctgccgcact tgcccaccgc ttcgaggatg gcccggttgc
     3301 ttttgcgata tgcccccagc gtatcggttt gatcgaaagc ggcaatgaga ggcgcctcgc
     3361 cggcagcagc gccggtttgg ccgatggcat acgctactgc agcgcgtacc tcgtccacag
     3421 ggtcggccag caattcggtc aatttcggta cggcggcagt atctttgatg gaagcaaatg
     3481 ccagggccgc ggcatagcga tagttagggt tatcgttggc caaccattgg tagaggctgt
     3541 cggattggcg gcggtcctgc aagtcataaa tttgcctgaa cacagggtct ttcaggtcaa
     3601 tatccacatc ggtgaacgta tcgtcttttt ggtaaggacg gcaggcggcc agggccaaaa
     3661 ggaaaaagag agccgaccag aaggcagttc tgagcatgtt ttttaagtat tgaatggcaa
     3721 atttattcat cttccccctc cggcttttca tcgccgaaaa tgtcattgga aaattcgtcg
     3781 tccaagtctt taaatgccgg gcgcccggag gagtatttgg agcagtccaa ctcgatgtcc
     3841 atatctcccg gcgggcgggc aaagcgcaca gaagcatcgt aatctatgcc gggcgcttct
     3901 tcgagggcct tgatgaaagc tgaaaagaat ggcttggaca tgtacgagcc ttgcccattg
     3961 gctatagaca ggaagcgaat ccagcggtct tctccgccta cccaggtacc cactaccaaa
     4021 cgcggcgtga cccccatgaa ccagccgtcc acgtagtcgt tggtggtacc cgttttaccg
     4081 cccacttcgc ttttgatgcc gcccatattg gtggcggagt acttgagcaa atcgagcatg
     4141 gcgtagttgg cgctgggcga cagggcctgc ttctcttccg gcatggcgct gtaaatgacc
     4201 ctgccgttgc ggtcttcgat gcgggtgaca aaaaccggct tcacatacat cccgttgttg
     4261 gcaaacgtag tgtaggcgcc cgtcatttcc atcacagata agtctgtggc gcccaggcag
     4321 atagccggag acttgggcac ccgatgccgc ccgttggggt agcgcgtagt gctgtctatg
     4381 cccatcatgt gtaccaaacc gcgaatgggt tctacatcgc cgatttcttt gacgagatgc
     4441 acagagaccg tgttgacgga attgcgcaaa ccgtcgcgca ggttgaaagt ctttcccgaa
     4501 taaccgcctg aagcattggc gggcgtccnn nnnnnnnnga ctggaacaag ccgaaattgc
     4561 cctctccggg cgatatggtt ttggccacat cggccacggt ataacagggc gagaaacgat
     4621 atatctctat ggcggtggca tatacaaaag gcttgaaagt agagcctacc tgccggttga
     4681 tggtggcgtg gtcgtattgg aaatacttgt gattgatgcc gcctacccac acttttacat
     4741 agccggtagc gggatcaacg gccagaatgc ccgtttgtaa aaacatacgg tggtagcgaa
     4801 tggaatccaa tggagacatc acggtgtctt tttccatttc ctcgttgtag gcgaatacgc
     4861 gcatggctac cggcgtactg aactcacggt cggaggcgtc ctgtagtttg ctccaggcgg
     4921 tctgaagttg agacaggtga ctgctgcgga gtacattttt gagtcttcgg gcggtttcct
     4981 cgctcagttt tttggctttc accaaacggg ccaatgaatc gggccgggca gcttcggcca
     5041 tcacgagttc cacttcccag tcatcgttca gcagcttgtc gtcgcccatt tcggcgcgca
     5101 ctttttcggc cacttccagc aggtatttat cgcgcagggc ctgatagcgc tcggaggcgc
     5161 ggacaagccc cttgagtctt tgtgctcgta cggctaccgg aatttcggtt tcagacccct
     5221 gcgagtactt ccagggatcg cgtttgttcc aggttttgaa aaaagcatcc tgcaccttgg
     5281 ccatgtggcg ggccatgacc tcctctgcaa ttttttgcat ttgcgggtct atggtggtat
     5341 aaattttgag gccgtcgcga taaatatcgt agggcgtgcc gtcgggtttg tagtattgtt
     5401 ctttggccaa gatttcctct ttgacatact tgccgagttc catacggaaa tacggagcca
     5461 ggccgtctat gtgcgtttgg cgggtgaagc ggaggcccaa aggcagtttt ttgagagact
     5521 ccagttcatc tttgcgcaag aggccgtttt tgtacatttg aagaaaaacg acattgcggc
     5581 gttgttgcac ctgctccggg cggcgcagcg gattgaacag cgaggggttt ttgagcatac
     5641 cgatgagcat ggcggcctcc tgtacttcga gcgagtcctg tgggcggtcg aagtagattt
     5701 cggaggctgc cttgatgccg taagcgccat tgatgaagtt gaatttattg agatacatgg
     5761 ccaatatctc ttctttggtg tagctgcgtt ctaagcgcac ggcgatgatc cactcgcgca
     5821 gtttttgcaa tacgcgcccc cacccttcgg ctttctggcc ggtgaacaaa agtttggcca
     5881 attgctgcgt gatggtgctg gccccgccgg aacctttttt ctgcaatgca atggttttga
     5941 ccgctacgcg ccccaatgcc tggaaatcta tgccggtgtg tgagtaatag cgctcgtctt
     6001 cggtggcaat gagcgcattg accaagtgcg gagagaggtc ttcataaccc accggtacgc
     6061 ggttttctat ataataacgc cctatttctg tgccggagga gccataaacc accgaggcca
     6121 attcgctctt ggggttttcc aactgactga cggagggaag attggtaaac gacaacaata
     6181 caaaaatcaa aacaacggcg ccaatgccga taaacagcag ccgccacatc catacagccc
     6241 agttgcgata agaggaccgc tcggtttgga acaaaaaatc agtgatggtt tgccacatgg
     6301 tatgttggtt tgatggtgcc ggccgggtgg gcaacgactc aatgggggca aaattaagga
     6361 ttatggagaa ttattccttt aatattaa
//
LOCUS       DAORLF010000283         6387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6579_length_6387_cov_1.639608, whole genome shotgun sequence.
ACCESSION   DAORLF010000283 DAORLF010000000
VERSION     DAORLF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6579_length_6387_cov_1.639608"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>659
                     /locus_tag="PK198_18800"
     CDS             <1..>659
                     /locus_tag="PK198_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40851.1"
                     /translation="RRWLLSPAHNLREDLPKLFDALCQYEDPEQNRAQVWAEVFPDAP
                     FSDQEYRLRCSYLLGVLEDWLVWQRRRDRSPDSGAILLASYRERGLERHFNRRLPRER
                     ELLERLPLRTPEFYLAQYQLEREFYLEDSKGERLRPKNFQEQDDALTCALLSMKLRQA
                     CLALAHERVFETGYRMAFVDEALQWAQKQPYADAPAVARHDGVDEGLEGAEVGGRAGV
                     A"
     assembly_gap    660..737
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <738..>1102
                     /locus_tag="PK198_18805"
     CDS             <738..>1102
                     /locus_tag="PK198_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40852.1"
                     /translation="HINQGRVLYLQEALELYRLGLEGNLLTEQGYLTPYTYNNIAGIA
                     LRLAEFDWAEEFVHRYQFQLSPEHREPLYALNAARLAYARKQYREALRFLQSADYRDF
                     FHQMTARIMQLKIYFETGE"
     assembly_gap    1103..1112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1113..1324
                     /locus_tag="PK198_18810"
     CDS             <1113..1324
                     /locus_tag="PK198_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40853.1"
                     /translation="LGAHLKNTRALLRRRRTSYHEQNYLNIIRLTEQILRLRPNDANG
                     RAALRHKMETLEPLTERAWLLAVLG"
     gene            complement(1399..1821)
                     /locus_tag="PK198_18815"
     CDS             complement(1399..1821)
                     /locus_tag="PK198_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRF40854.1"
                     /translation="MQYWLVKSEPSVYPFEELERTGRTMWEGVRNFAARNHLRAMKNG
                     DLVLFYHSREGLEVVGVCRVVQEFYPDPTAESGDWSVVDLAPVKRLHKPVPLSVIKAV
                     PELQQIGLVRIGRLSVMPLQEAEFQKIMELGETDFSGQ"
     gene            complement(1823..2356)
                     /locus_tag="PK198_18820"
     CDS             complement(1823..2356)
                     /locus_tag="PK198_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRF40855.1"
                     /translation="MRKYSTEELGRPDAATFREQPKLPITIVLDNVRSALNVGSAFRT
                     CDAFAIEEIILCGISAAPPHREIAKTALGATETVAWRYFADTAEAIATMQSEGRQIWV
                     VEQTEGSTALQDFIPQSEARYALVFGNEVHGVEEAWIEQARGALEIPQWGAKHSLNVS
                     VCVGVVLWEIVRKIKFA"
     gene            complement(2424..2496)
                     /locus_tag="PK198_18825"
     tRNA            complement(2424..2496)
                     /locus_tag="PK198_18825"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2461..2463),aa:Lys,seq:ttt)
     gene            complement(2744..3340)
                     /locus_tag="PK198_18830"
     CDS             complement(2744..3340)
                     /locus_tag="PK198_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRF40856.1"
                     /translation="MMIQKEGYIKFEARWTLAPPLPDADIAEINHWRQHVFELGFIGA
                     YPDGIGFGNISRRWDAARFLISGSATGHLDALDARHYCLVHSADPEQNLVCCEGPVIA
                     SSESMSHAVIYRHCPEVGAVVHAHHRALWERWMHRLPTTGAGVSYGSPEMAAEIVRLL
                     RETELRHSGVFITAGHEEGIFAFGRNLAEAVQRLGQLK"
     gene            complement(3419..>3986)
                     /locus_tag="PK198_18835"
     CDS             complement(3419..>3986)
                     /locus_tag="PK198_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40857.1"
                     /translation="ADVLIINKIDSASPEGIERVRAAIERLNPAATVIDAASPIKVED
                     PAVIRGKRVLAIEDGPTLTHGGMTFGAGTLAAKKFGAAELVDPRPYLSGKIIHTFLEY
                     PGIGTLLPAMGYGDEQLRDLETTIEQTDCEAVVIGTPIDLRRVIRIDKPSTRVFYDLQ
                     EIGVPTLNEVLDSFLEKKQIGRLETVSV"
     assembly_gap    3987..4175
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4176..4783)
                     /locus_tag="PK198_18840"
     CDS             complement(<4176..4783)
                     /locus_tag="PK198_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40858.1"
                     /translation="MKKKRILIIGAAGRDFHNFNTLYRNDNRVEVVAFTAAQIPDIDG
                     RNYPPELAGPLYPAGIPIYSQDDLPNLIRSLRVDECVFSYSDVSNQYLMELSALVQAA
                     GASFVLPGPAATMLRSTKPLISVCAVRTGSGKSQTARLIVELLMQRGLRVVAIRHPMP
                     YGDLVRQAVQRFARIEDLQKHHCTIEEMEEYEPHIVRGNVIYA"
     gene            complement(4942..5280)
                     /locus_tag="PK198_18845"
     CDS             complement(4942..5280)
                     /locus_tag="PK198_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40859.1"
                     /translation="MTAKNNLTAEPLAPAAYIKPMLLGAGIALLLIIAFLSGVDHPDP
                     AWPKYWMARPLIIVPLAGAAGGAFFALLRPLRRRGGWVMAFAYVLSLVIFVVALWMGT
                     VLGLDGTLWN"
     gene            5371..5532
                     /locus_tag="PK198_18850"
     CDS             5371..5532
                     /locus_tag="PK198_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40860.1"
                     /translation="MLQIPANALNFVCVNHIPMTDEKKLPDKGPLQATFLYDILPQAL
                     LLPHPLLRF"
     gene            5642..>5910
                     /locus_tag="PK198_18855"
     CDS             5642..>5910
                     /locus_tag="PK198_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40861.1"
                     /translation="MRKLLPFLGILLLVLPFGLSGQVYIWVGGNMTAPGPNAWNNPGN
                     WLSYHMGATYPQLTTDLALFISASPGYPTVQLNSNIEIGRLVAATG"
     assembly_gap    5911..5991
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6033..>6387
                     /locus_tag="PK198_18860"
     CDS             6033..>6387
                     /locus_tag="PK198_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40862.1"
                     /translation="MLSDIFFDKTGIGVTNFQNGNILNFSNPSNAIRLSSPSAAGHIN
                     MGITSGDTFTGGARFINNSNATLTIGFQGTSTFSGNVELAQSAMGGIVFGGMGGTSTL
                     TAGGLNASAVTAGTLS"
BASE COUNT         1397 a         1672 c         1592 g         1368 t
ORIGIN      
        1 cccgccgctg gctgctttcg cccgcgcaca acctccgcga agacctgccc aaactcttcg
       61 atgccctgtg ccaatatgaa gacccggagc aaaacagggc gcaagtctgg gccgaagttt
      121 ttccggatgc ccccttcagc gaccaagagt accgcctgcg ttgcagctac ctgctgggcg
      181 tactcgaaga ttggttggtg tggcagcgtc gccgcgaccg ctcgcccgat tccggggcca
      241 tcttgctggc gtcttaccgg gaacggggcc tcgaacggca tttcaaccgc cgcctgcctc
      301 gcgaacggga gttgctggaa cgcctgcccc tgcgcacgcc ggagttttat ctggctcagt
      361 atcaattgga aagggagttt tatctggaag acagcaaagg cgagcggctg cggccaaaaa
      421 actttcagga acaagacgac gccctcacct gcgccctgtt gagcatgaaa ctccggcagg
      481 cttgtctggc cctggcccac gagcgcgttt ttgaaaccgg ttatcgcatg gcttttgtgg
      541 acgaggcttt gcaatgggcg caaaaacagc cctacgccga tgcgcccgcc gtagcgcgac
      601 acgatggcgt cgacgaaggt ctggaaggtg ccgaagtcgg cgggcgggct ggtgtcgccn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnngcc acatcaatca ggggcgggtg ctgtatcttc aggaggcgct
      781 ggagttgtac cggctgggct tggagggcaa tttgctcacc gagcagggct accttacacc
      841 atatacatat aacaacattg ccggcattgc cctgcggctc gcagagttcg actgggccga
      901 ggagtttgtt catcggtatc agtttcagtt gtcgccggaa caccgggaac cgctgtatgc
      961 actcaacgcg gcccgactgg cctacgcccg caagcaatac cgggaggctt tgcgcttttt
     1021 gcaaagtgcc gactaccgcg attttttcca tcaaatgacg gcccgcatca tgcagttgaa
     1081 aatttatttt gaaactggag aannnnnnnn nntgctcggc gcgcacctca aaaacacccg
     1141 cgccctgctg cgccgccgcc gaaccagcta tcacgaacaa aactacctga atatcatacg
     1201 actgaccgag caaatactgc ggcttcggcc caacgacgca aatggccggg cggccttgcg
     1261 ccacaaaatg gaaacattgg agccactcac cgaacgggcc tggctgctgg cggtgctggg
     1321 ttgaatcggt aggcggtagg cagtcggcgg ttccagcgga gcgtgtactg ccccaggtaa
     1381 ccgcctaccg cccgccgcct actgcccact gaaatccgtt tcccccaatt ccatgatttt
     1441 ctgaaattcc gcctcctgca aaggcatcac agagaggcgg ccaatgcgca ccagaccaat
     1501 ttgttgcaat tccggcacag ctttgatgac actgagtggt actggtttat ggagacgttt
     1561 tactggggct aagtccacca ccgaccagtc gcccgattca gcggtgggat cggggtaaaa
     1621 ttcctgcacc acgcggcaca cacccaccac ttccagcccc tcccgactgt ggtaaaacaa
     1681 caccagatcg ccgtttttca tagcgcgcag gtggttgcgg gctgcaaaat tgcgcactcc
     1741 ttcccacatc gtgcgaccgg tgcgctccaa ttcctcgaac ggataaacgg aaggctcgga
     1801 tttgaccaac cagtactgca tatcaggcga atttaatttt gcgaacgatt tcccaaagga
     1861 ctacccccac acaaacagac acattgagcg aatgtttggc gccccattgc ggtatttcca
     1921 gcgcgccacg ggcctgctct atccaggctt cttccacgcc atgcacttca tttccaaaaa
     1981 ccagagcgta ccgggcctcg gattgcggaa taaaatcctg caaagccgtg ctgccctcag
     2041 tttgctccac cacccatatc tgccgccctt cggattgcat ggtcgcaatg gcttcggcgg
     2101 tatcggcaaa atagcgccag gctacggttt cggtagcccc cagagcggtt ttggctattt
     2161 cccggtgtgg aggtgcagcg ctgatgccgc agagaatgat ctcctctatg gcaaaagcat
     2221 cgcaggtgcg gaaggccgaa cccacattga gtgccgagcg aacattgtcc aacacaatgg
     2281 taatggggag tttgggttgt tcccgaaaag tggccgcatc gggcctgccc agctcttctg
     2341 tgctatattt gcgcatgaaa aatggaattc ggtaaataaa aaaagtcctt gcaaattatt
     2401 gatttacaag gacttttgag aagtgattcc gctgggattc gaacccagga cccatacatt
     2461 aaaagtgtat tgctctaccg gctgagctac ggaatcaact gctttttcaa aaagcgatgc
     2521 aaagataagt ctgtttttga taataaagtg catgttgaag aaaatttttt ttcgtgtggc
     2581 tttaatagca ggccaaaagc ggacgattgt ttggaacaca caggggaaaa agttttcact
     2641 atactttcta ccggcctccg cctctgttat ctttgaggct tcaaaaaaag tacattatgt
     2701 acggctatca agtgttgaac tatgccattt ccggcaatgg ttttcacttc aattgcccca
     2761 ggcgctgcac cgcctctgcc aaattccggc caaaagcaaa aataccttcc tcgtggcctg
     2821 cggtgataaa aacgccgctg tgtcgcaatt cggtttcgcg caagagccgc acaatctccg
     2881 ccgccatttc cgggctgccg tagctcacgc ctgcgcccgt tgtgggcagt cggtgcatcc
     2941 agcgctccca cagcgcccgg tgatgcgcgt gtacaacagc ccccacttcc gggcaatgcc
     3001 ggtaaatgac ggcatgactc atggattcag acgaggcaat gaccggacct tcgcagcaga
     3061 cgaggttttg ctccggatcg gcagagtgaa cgaggcagta atggcgggca tccagcgcat
     3121 ccaaatggcc ggtagccgag ccggaaatga gaaagcgtgc cgcatcccac ctccggctga
     3181 tattgccaaa cccgatgccg tcggggtagg cgccgataaa ccccaactca aagacgtgct
     3241 gccgccaatg gttgatctcg gcaatgtccg cgtccggcag cggaggcgca agcgtccatc
     3301 gcgcctcaaa ttttatataa ccttccttct gtatcatcat ttactctcac ctctctccgc
     3361 tggaagatca actgccgcaa gcagattatg gcaaagccga taaactgtta ctgtcggctc
     3421 aaacgctcac ggtctccaac ctcccgattt gctttttctc caaaaacgaa tccaacacct
     3481 cgttcaatgt gggcacgcct atttcctgca agtcataaaa cacgcgggtg ctgggcttgt
     3541 caatacgaat cacgcgccgc aaatcaatgg gcgtaccgat gacaacagcc tcacagtccg
     3601 tctgttcgat ggtagtttcc agatcacgga gttgttcgtc gccgtagccc atggccggca
     3661 gcagcgtacc gatgcccgga tattcgagga aggtatgaat gattttgccg cttaggtacg
     3721 gacgcggatc caccaattcg gcagcgccga actttttggc agccaatgtg cccgccccga
     3781 atgtcattcc gccgtgcgtg agggtaggcc cgtcttcaat ggccagtacg cgtttgcctc
     3841 tgatgacggc cgggtcttcc accttgatgg gcgatgcggc gtctatcacg gtagcggccg
     3901 ggttgaggcg ttcaatggct gcgcgtaccc gttcgatgcc ctccggcgag gcgctgtcta
     3961 tcttattgat aatgagcaca tcggccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgta gatgacattg ccgcgaacga
     4201 tgtgcggttc gtactcctcc atttcttcaa tggtgcagtg gtgtttttgt aagtcttcaa
     4261 tgcgtgcgaa gcgctgcact gcctgccgaa ccagatcgcc gtagggcatg gggtgccgga
     4321 tggccactac ccgaagtccg cgctgcatga gcagttccac gatgagccgg gcggtttggc
     4381 tcttgcccga accggtgcgc acggcacaaa cactgatgag cggtttagtg ctgcggagca
     4441 tggtagcagc cgggccgggc aggacgaaag aagcgccggc ggcctgtacc agcgcgctca
     4501 gttccatcag atattggttg ctcacgtcgc tgtacgagaa cacgcactcg tccacgcgga
     4561 ggctgcggat gaggttgggc aaatcatctt gagaatagat ggggatgcct gccgggtaga
     4621 gcggtccagc cagttcaggc gggtaattcc gtccgtcaat atcgggaatt tgagcggcgg
     4681 taaaggctac tacttcgacg cggttatcgt tgcggtacaa agtgttgaaa ttgtggaaat
     4741 cgcgcccggc ggcgccgatg atgaggattc ttttcttttt cacggcagta gtgcttttgg
     4801 ttaggaaagt ggcctcaata tcagaaaatt actgcctgcc gggcaatgac tttggtcagg
     4861 gtttgaggtg gacttttatc ttgtcaactc gtagtgaagg gctggctgcc agtcagccct
     4921 tcactacccc cgactcaata atcagttcca caaagtaccg tccaggccca gcaccgtccc
     4981 catccacaaa gcgacaacaa agatcaccaa actcaataca taagcgaagg ccatgaccca
     5041 gccgcctcgc ctgcgcaggg ggcgcaagag ggcaaaaaat gctcctccgg cagctccggc
     5101 caatggtacg atgataagcg gcctcgccat ccagtatttc ggccaggccg gatcggggtg
     5161 atccacaccc gacaaaaacg cgatgatgag cagcagcgca atgccggcgc ccaacagcat
     5221 gggcttgatg taagcagccg gagcgagcgg ctcggcagtc agattgtttt tggcagtcat
     5281 aatgcggtga ttttgaattt aaaagtactt tacaatgcaa agtaaataaa gattactcaa
     5341 cctgccaaga aaaaataaaa aaagctgcac ttgttgcaaa tcccggccaa tgcgcttaat
     5401 tttgtgtgcg ttaatcatat tcccatgacc gatgaaaaaa aactgcccga caaagggcct
     5461 ctgcaagcga cgtttttata cgacattcta ccgcaggcac tgctcctgcc tcacccttta
     5521 ctaaggtttt aatgctgcaa ccggcacctt gccgaagggc agttttttgt actgaaaaca
     5581 tgatttaaca acatactatt cacactccgt tcttttcttt cacgaacaaa aattgacaac
     5641 tatgagaaag cttttaccat ttttggggat tcttctcctt gttttgccat ttggcttgtc
     5701 gggtcaggtc tatatatggg tgggaggaaa tatgactgct cctgggccta atgcctggaa
     5761 caatccgggc aattggttga gctaccacat gggagcaacg tatccccaat taacaactga
     5821 tcttgccctg ttcatttccg cctctccagg gtacccaacc gtccaattga actcgaatat
     5881 tgaaattgga agattggtgg ctgccactgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggtacatg
     6001 gtggctcagg aacactgaat atctccgggg ccatgcttag cgatattttt tttgacaaaa
     6061 caggaatcgg cgttactaat tttcaaaacg gaaatatctt aaatttttct aatcccagca
     6121 atgccatcag actgtcatcg ccatcagctg ccggccacat aaatatgggt atcacctctg
     6181 gagatacttt tacaggcggc gccagattta tcaacaacag caatgctact cttaccatag
     6241 gttttcaggg aaccagtacg ttcagcggca atgttgaatt agcgcaaagt gctatgggag
     6301 ggattgtatt tgggggcatg ggggggactt ctacgcttac tgcgggcggc ttgaacgctt
     6361 ccgccgttac ggccggaacc ttgagct
//
LOCUS       DAORLF010000284         6356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6622_length_6356_cov_2.116331, whole genome shotgun sequence.
ACCESSION   DAORLF010000284 DAORLF010000000
VERSION     DAORLF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6622_length_6356_cov_2.116331"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK198_18865"
     CDS             <1..401
                     /locus_tag="PK198_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40863.1"
                     /translation="LTEFSNVLPSCYSVNELLPPRLYTPVGVFFDLQQVSSGLRRLRS
                     NLPDQLWERTAKILRLNLFEQIFEQIFFHKKFTFYCGKLHLKLICFYPRFSLCAHLFR
                     KADAKVSTPDSFVQVFFKIFFIIYQNEPGV"
     assembly_gap    528..565
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(581..3424)
                     /gene="uvrA"
                     /locus_tag="PK198_18870"
     CDS             complement(581..3424)
                     /gene="uvrA"
                     /locus_tag="PK198_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF40864.1"
                     /translation="MAKRTTAEVAEKGAFRDKIFIKGARANNLKNIDLEIPKNQLVVV
                     TGVSGSGKSSITMDTLYAEGQRRYVESLSAYARQFLMRMKKPDVDYIKGICPAIAIEQ
                     KVSTANARSTVGTLTEIYDYLRLLYARVGKTYSPVSGNVVKKHEVSDVTSYIQSCNNG
                     ARVQLFIPLPYKYKDRVLASELDLLLQKGYTRIWKDGALQQIQDVLESGEPFLKKQLH
                     EIHGSDIRILVDRFAVSHDDAENLKRVADSVQTAFYESEGECVVEVENQPAQFFNNRF
                     ELDGMSFPEPTPQLFNFNNPFGACPVCEGFSMVMGIDEDKVIPDKTLSVYQGAVACWK
                     GEKHGLWLDHFLSVAHHFDFPVHRSYQDLSQAEQKLLWDGNDKFEGIHAFFDELERNT
                     YKIQNRVMLARYRGRTVCRACGGGRLRKEASYVKIGGKAISELTVIPIAHLPVFFENI
                     QWSEYELQVAKRLLLEIGNRLKFMNQLGLGYLTLNRLSSILSGGETQRINLTRTLGSN
                     LTSSMYLLDEPSVGLHPRDTGRLIEVLRSLRDLGNTVVVVEHEEDVIKNSDYLVDIGP
                     KAGIFGGEVVFAGPYHHIYKEAGESLTTKYMSGRMAIPVPAVRRKLSDFIQITGARQH
                     NLKNITARIPLHALTVVAGVSGSGKTTLVRDILYPALMRQLGQSTPKAPGLHTALDGD
                     WKRITHIEMINQSPIGKSSRSNPVTYVKAYDAIRDLMTGQQLSKIRGFKPKHFSFNVE
                     GGRCETCKGDGEIVVEMQFLADVHLECEDCRGRRFKDEVLDVLYKGKNIFEILNLSVD
                     EAIEFFADVKDVVSKLQPLADVGLGYIKLGQSSSTLSGGEAQRVKLASFLGKERSDER
                     ILFIFDEPTTGLHFHDIRKLLDALNALVEKGHTVLVVEHNMEVIKCADWVLDLGPEGG
                     EAGGYLVFEGRPEDLAQTASSYTGHYLKEKLTV"
     gene            complement(3695..>4619)
                     /locus_tag="PK198_18875"
     CDS             complement(3695..>4619)
                     /locus_tag="PK198_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF40865.1"
                     /translation="ALDLGVFHRKNEIVTTRSALRWTGLWVTVALSFAIFVYHAYTQG
                     WVANPGGLSGREAVVLYITGYLIEESLSMDNIFVIALIFNYFMVPKEYQHRVLFWGIL
                     GALVFRGLMIGIGAALVQRFDWMVYVFGALLMYSAYKMLRSGEEDINPGKNPILRIIG
                     KFYPVLKQFKGDRFFVRLPVKDRKVWAVTPLFVTLMVVETTDVMFAVDSIPAIFAITT
                     DPFLVFTSNIFAILGLRSLYFVLASLLDKFSYLKYSLIFILFFVGVKILISHWVHLPA
                     AISLGIIALALASGIVFSLLKASQSEEQAGS"
     assembly_gap    4620..4631
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4816..6336
                     /locus_tag="PK198_18880"
     CDS             4816..6336
                     /locus_tag="PK198_18880"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRF40866.1"
                     /translation="MATKGKSRIDRFLGVIERLGNALPHPATLFAGFALFIVLLSGFL
                     SLFNLEVIHPGTKETVTVTNLLSVAGLHRILTGMVTNFTNFAPLGTVLVALLGIGIAE
                     GSGLIGTALRLIVLKSPPRLLTYVIVFAGVISNTASEVGYVLLVPLAAVIFLAAGRHP
                     LAGLAAAFAGVSGGYSANLFLGTIDPLLAGLSQEAARIVQPGYEVNPACNYYFMAAST
                     FLITLLGTWVTEKLVEPRLGQYNGDEKPEEVKPLSPEERRGLRFTLIAAVLFTAWVMF
                     GLIPENGFLRDPETHSILKSPFMSGIVALLFLGTALLGIAYGWGARTIKSDEDVMKGM
                     GKSMQTLGSYIVLVFFAAQFVAYFNWSNIGLLVAVKGAEGLRASGLGPIPLMISFVAV
                     SALINLAMGSASAKWAIMAPVFIPMFMLLGYTPEFTQAAYRVGDSVTNIISPMMSYFA
                     LIVAFIQRYDKGAGIGTVVSTMLPYTVVFFVGWVSLLIIWILLGLPLGFGAGMFME"
BASE COUNT         1441 a         1479 c         1757 g         1629 t
ORIGIN      
        1 ttcttaccga gttttctaat gtcttacctt cttgctacag tgtcaatgaa cttttgccgc
       61 ctcgtctcta tactcccgtt ggagtatttt ttgatttgca gcaggtatct tcaggcttgc
      121 gccgattaag atcaaatttg cctgaccaac tttgggaaag gactgcaaag atactacgct
      181 tgaatttgtt tgagcaaatt tttgagcaga ttttttttca caaaaaattc acattctatt
      241 gtggaaaact tcatttgaag ttgatctgct tttacccgcg tttttctttg tgtgctcacc
      301 tctttcggaa agcggacgca aaggtaagca cgccggattc atttgtgcaa gtgtttttta
      361 aaattttttt cattatttat caaaacgaac ccggtgtgta gaaaatataa ttcggcgaaa
      421 gccttccatt tttacttttt atcagacaat cgctgaaaga aagccctgct tgaaagatgg
      481 cctgaaatga aaagataaga ggaaattttt tttagtttca agggcttnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnntggga gtaagacggc tcagacagtc agcttctctt
      601 tgaggtaatg ccctgtgtag gaagacgccg tttgggccaa gtcttcaggg cggccttcaa
      661 atacaaggta tccgcctgct tcgccgcctt cggggccaag atcgagtacc cagtcggcgc
      721 atttgatcac ctccatgttg tgttctacca ccaaaacggt gtggcctttt tctaccagcg
      781 cgttcagtgc gtccaagagt ttccggatgt cgtgaaagtg aaggccggtg gttggctcgt
      841 cgaatataaa gaggatgcgt tcgtcggagc gctctttacc gaggaaggaa gcgagtttga
      901 cgcgttgggc ttcgccgccg gaaagcgtgc tggaggattg ccccaatttg atgtacccaa
      961 gcccgacatc ggccagtggt tgtagtttgg atacgacgtc tttgacatcg gcaaaaaact
     1021 cgatggcttc gtccacactc aggttgagta tttcaaagat gtttttgcct ttatacagca
     1081 catccagcac ctcgtctttg aaacgccgcc cccggcagtc ttcgcactct aagtgtacat
     1141 cggccagaaa ctgcatttcc accacaattt ccccatctcc tttgcaggtt tcacagcgac
     1201 cgccttccac attaaaagaa aagtgtttgg gtttgaagcc ccggattttg gaaagctgtt
     1261 gaccggtcat caggtcgcgg atggcgtcgt aggctttgac gtaagtgacc ggattggaac
     1321 gggaggactt gcctatggga ctttgattga tcatctcgat gtgcgtaatg cgtttccagt
     1381 cgccgtcgag agcggtgtgg aggcccggag ctttgggtgt gctttggccc aattggcgca
     1441 tgagcgcggg atagagaata tcgcgtacca gcgtggtttt gccggaaccg gaaactccgg
     1501 ctaccaccgt gagcgcatgt agcggaatgc gtgctgtgat gtttttgaga ttgtgctgcc
     1561 gggcgccggt gatctggatg aaatcgctga gtttccgacg gacggcgggc acggggatgg
     1621 ccatgcgccc actcatgtat ttggtggtaa gactttcgcc ggcctcctta tatatatggt
     1681 ggtaaggccc ggcaaagacg acctctccgc caaagatgcc tgctttgggt ccgatgtcca
     1741 ccagataatc gctgtttttg atgacgtctt cttcgtgttc tacgaccacg acggtgttgc
     1801 ccagatcgcg cagggaacga agcacctcga tgaggcggcc cgtatcgcgg gggtgcagcc
     1861 ctacgctggg ttcgtccaag aggtacatgg agctggtgag attgctgccg agtgtgcggg
     1921 tgaggttgat gcgctgggtt tcgccgccgc tgaggataga ggagaggcgg ttgagcgtga
     1981 gatatcccaa gcctaattgg ttcataaatt tgagccggtt gccgatttcg aggaggagtc
     2041 gtttggctac ctgcaattcg tactcgctcc attggatgtt ttcaaaaaaa acaggcaggt
     2101 gggcgatggg aatgacggtc agttctgaaa tggctttgcc gccgattttg acataggatg
     2161 cctctttgcg caaacgcccg cccccacagg cacggcagac ggtacgcccc cgatagcggg
     2221 ccagcatgac gcggttttgt atcttatagg tattgcgttc caactcgtca aaaaaggcat
     2281 gaataccctc gaacttatcg ttgccgtccc aaagcagttt ttgctccgct tggctcaggt
     2341 cttgatacga ccggtgtacc ggaaagtcga aatgatgggc taccgagagg aaatggtcta
     2401 accagaggcc gtgtttttcg cctttccagc aggcaacggc gccttggtaa acagagagtg
     2461 ttttgtcggg gatgactttg tcttcatcaa tgcccatgac catgctgaag ccctcgcaaa
     2521 cggggcaggc gccgaatggg ttgttgaagt tgaaaagttg gggcgtgggt tcggggaagc
     2581 tcatgccgtc caactcgaag cgattgttga aaaactgagc cggttggttt tccacttcca
     2641 ctacgcactc gccttcactt tcgtaaaaag cagtttgaac agaatcggct acgcgtttga
     2701 ggttttcggc atcgtcgtgg ctgacggcaa agcggtccac cagaatgcga atatcggagc
     2761 cgtgtatttc atgaagttgc tttttcagaa agggttctcc ggattcgagc acgtcctgaa
     2821 tttgttgcag ggcgccgtct ttccagattc tggtgtaacc tttttgaagg aggaggtcga
     2881 gttcggaggc gagtacgcga tccttatatt tatatggcag cggtatgaaa agttgtacgc
     2941 gggcgccgtt gttgcaactt tggatgtagc ttgtgacatc ggaaacctcg tgttttttga
     3001 ccacattgcc ggaaacgggc gaataggttt tcccaacgcg ggcgtagagc agccgcaggt
     3061 agtcgtaaat ttcggtgagg gtgccaacgg tagaacgcgc attggcggtg ctcacttttt
     3121 gctcgatggc gatggccggg cagatgcctt tgatataatc tacatccggt tttttcatgc
     3181 gcatgagaaa ctggcgggcg taggcagaaa gactttccac gtagcggcgc tggccctcgg
     3241 cgtagagggt gtccattgta atagaagatt tgcccgaacc ggaaacaccc gtgacgacga
     3301 cgagctggtt tttgggaatc tctaagtcta tgtttttcag gttgttggct cttgcgcctt
     3361 tgatgaatat tttatcgcgg aaagcacctt tttcggcaac ttcggcagtc gttcgtttgg
     3421 ccataatttt cgtcgagtga gcgggggtat aacaaaggcc gcaagggaat ggttgagggg
     3481 ccgagcggaa cgtatattac tattcagcag gtctccaaaa gctacgagct acgagccgtg
     3541 agctatgagt ctgttttctc gttcataaat tagattttcc attcggggaa attcaaaata
     3601 tcctattgga aataaggctc tttttgattt tgaaaaatca aactcgcagc tcgaaactcg
     3661 cagctcgcgg ctttttgcag ggcgaatgga tggtttagct acccgcctgc tcctctgatt
     3721 gagatgcttt gagcaaagaa aatacaatgc ccgacgccag agccaacgca atgataccca
     3781 gagatatggc agccggcagg tgaacccaat ggctgatcaa aattttgacg cccacaaaaa
     3841 acaggataaa aatgaggctg tacttgaggt agctgaattt atcgagcagg gaagccagaa
     3901 cgaaatacag cgagcgcagg cccagaatgg caaagatatt ggacgtgaac accaaaaagg
     3961 ggtcggtggt aatggcaaaa atagccggta tgctgtcaac ggcgaacatg acgtcggttg
     4021 tttcaaccac cataagggtt acaaacaaag gcgtgacagc ccatactttg cggtctttga
     4081 cgggcagcct gacaaaaaaa cggtcacctt tgaattgctt gagcaccggg tagaatttac
     4141 cgatgatgcg caaaataggg ttcttgccgg gattgatgtc ctcctcgcct gaacgcagca
     4201 ttttgtaagc agaatacatg agcaatgccc cgaaaacgta caccatccaa tcgaaacgtt
     4261 gcaccaaagc agctccgata ccgatcatca gtccccggaa taccaaggcc cccagaatgc
     4321 cccagaagag cacgcggtgc tgatactcct tgggcaccat gaagtagttg aaaatgaggg
     4381 caataacgaa gatattgtcc atactcaacg actcctcaat gaggtagccg gtgatgtaga
     4441 ggactacggc ttcgcgaccg ctcaggccgc cgggattggc cacccaaccc tgcgtatatg
     4501 cgtggtaaac gaagatggcg aaggacaaag ctactgtgac ccaaaggccc gtccaacgaa
     4561 gcgcagaacg ggtggtaaca atctcgtttt tgcggtgaaa aacgccgagg tcgagcgccn
     4621 nnnnnnnnnn naaaaataaa aatcaacgtg atgaatccaa tccagatggc cataatgtac
     4681 gggtggttcc gataagcgga acggcggcaa agataggtat tgattcagat actgtgccca
     4741 aaacttggcg acatggcgtg cagcttatat attcgcgctt tcttgaattc cctaacgaat
     4801 aaaaaccttt gcttcatggc cacgaaagga aaaagccgga ttgaccgatt tttgggcgtt
     4861 atcgaacgat tgggtaacgc gctcccccac cctgccacgc tgtttgcggg ctttgcttta
     4921 ttcatcgttt tgttgagcgg atttctgagt ctgttcaatt tggaggtgat acaccccggc
     4981 accaaagaga cggtcacggt gaccaacttg ctgtctgtgg cgggcctgca tcgcattctg
     5041 actggcatgg tgaccaattt taccaatttc gctccgctcg gcaccgtgtt ggtggcgctg
     5101 ttgggcatcg gcattgctga aggttcaggg ctgatcggca cggcactgcg actcattgta
     5161 ctcaaatcgc cgccgcgcct gctcacttat gtcattgtgt ttgcgggtgt tatctccaac
     5221 acagccagcg aagtaggcta tgtattgctg gtgccgttgg cggccgtcat ttttttggcg
     5281 gcagggcggc acccgttggc gggtttggcg gcggcgtttg ccggcgtgtc gggcggttac
     5341 agcgccaatt tgtttttagg aaccatagac cccttgttgg ccgggctttc gcaggaggcg
     5401 gctcgcattg tacagcccgg ttatgaggtg aatcctgcct gcaactacta ttttatggcg
     5461 gcatccactt ttctcatcac actattggga acctgggtaa cggaaaaatt ggtagaacct
     5521 cgcctcggcc agtacaacgg tgatgaaaaa cccgaagagg taaaaccctt gtcgccggaa
     5581 gaaagaagag ggctgcgatt tacgctcatc gcggcggtgt tgttcactgc ttgggtgatg
     5641 ttcggcctca ttcccgaaaa cggatttctc cgcgatcccg aaacgcacag tatcctcaaa
     5701 tcgccgttca tgtcggggat tgtggcgctg ttgtttctgg gtacagcgtt gctgggcatt
     5761 gcttacggct ggggggcgcg caccatcaaa tcagacgaag acgtgatgaa aggcatgggc
     5821 aaaagcatgc aaacactggg ttcttacatc gtgctggtgt tttttgcagc gcaatttgtg
     5881 gcgtatttca actggagcaa catcgggctg ttggtggccg tcaaaggcgc ggaaggactg
     5941 cgcgcttcgg ggctggggcc tattccgctg atgatttcgt ttgtggcggt ttctgcgctc
     6001 atcaatctgg cgatgggcag cgcttcggcc aagtgggcca tcatggcgcc ggtgtttata
     6061 cccatgttta tgctgttggg ctacacgccg gagttcacgc aggccgctta ccgcgtgggc
     6121 gacagcgtta ccaatatcat ctctccgatg atgtcgtatt ttgcgctcat tgtcgctttt
     6181 attcagcggt acgacaaagg cgcgggtatc ggcacggtgg tgtcaaccat gctgccctac
     6241 acggtggtgt ttttcgtagg atgggttagt ttgctcatta tctggatatt attaggtttg
     6301 cctctcggct tcggcgcggg tatgtttatg gaataaccat tgggtaagga aatgaa
//
LOCUS       DAORLF010000285         6353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6631_length_6353_cov_1.855827, whole genome shotgun sequence.
ACCESSION   DAORLF010000285 DAORLF010000000
VERSION     DAORLF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_6353_cov_1.855827"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK198_18885"
     CDS             <1..666
                     /locus_tag="PK198_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40867.1"
                     /translation="FKKIIPACNFAPQNPAFMKNLVLLMFSAVLLVAGCVNDKTSKSD
                     SEAADTAAVTNDPNVKFVPPAPLVDIDTSAARIGNGAYDFNALVPLFPDIIAGTGRQT
                     ANGENYKMAGGMISSASASYNVQDRRFSVAIQDVGDNADVIPQLAKWSTMAMNGDDEN
                     EVMRSTLYLNQPAFIHYNKASRSGSYSVIYSNRFVVDITGRNVELAELEKAMEDLRID
                     RLK"
     gene            742..>2602
                     /locus_tag="PK198_18890"
     CDS             742..>2602
                     /locus_tag="PK198_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40868.1"
                     /translation="MKKTITILLFWAMALPMIAQGTLQGKVTDEKGEPLIGVAVYERG
                     TDFGTSTDLDGKYSLTHSGPNAVITFSMLGFVTQEIKAGNQKTLDVKLLPDVSLLDQV
                     VVLGSRRSNRAQTETMAPVDVINIRQASMSTARFDVTSMMNYSAPSFNYNKQSGSDGG
                     DHVDLATLRGLGPDQTLVLVNGKRRHQTAFVAVFGTRGRGNSGTDLNAIPAASVERIE
                     VLRDGASAQYGSDAIAGVVNIILRRDTGFLNADLGFSVYHDPKYNPAYKHDLGLYVHG
                     NKLDGAAYTANVNYGLPLGKKGGFVNVGLQYADLAKTFRQELDGVLPESIYRRAHGDG
                     SMRGLGGMFNLEAPVKENDRMTFYAFGGYNSKNSDAYAFTRNFSARPERFPTKSDGEI
                     IPAPGIIFTDSGGEQYYNPRIRTEISDFSVAAGLKGISASGWNWDFSNTLGRNNFHFF
                     GEGTFNAGLGPDKTSFDDGGFSFLQNSVNLNFSKEVASILQGANFAFGAEARLENYQL
                     FAGEEASYRNYDPDKASGAQGFPGYQPSDEANANRSCIGVYGDVELDLTDAWLLGGAV
                     RIENYSDFGLTANYKLSSRMRLSPKFNLRGSVSTGFRAPSLQQINFSSTFTTVQ"
     assembly_gap    2603..2689
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2690..3501
                     /locus_tag="PK198_18895"
     CDS             <2690..3501
                     /locus_tag="PK198_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40869.1"
                     /translation="ERSFNANLGFTFKPVREFSITLDAYQVKVKDRVVLSGQFDAEDG
                     TLNPVFTAALQNLRVSLAQFFANAVNTTNRGIDIVLDYNKRRSNGHFRVLAAANFQQM
                     TIDQINMPTLLNDTPEHRATFLSDREQAFILASAPPVKINGTVEYGIEKFTLGARLNY
                     FGKIELLGYGEDGLGINPMVPTDADENIYVEDRYIYGGKLVPDLYIGWQASKSISLNF
                     GVDNILNVHPDLGFVPVASGWAFNNETGGPWDAVQMGGNGRRFFVRLGLNF"
     gene            complement(3602..5398)
                     /locus_tag="PK198_18900"
     CDS             complement(3602..5398)
                     /locus_tag="PK198_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40870.1"
                     /translation="MNKRISLYSLLICMAFLLAVGAIYYPKWNKGQTEATISWDVSGY
                     YFYLPAVFIYKDIHQVAFRDSIHQKYRPADAPYQAFQHPSGNYVMKYSCGLAVQYLPF
                     FLIAHALAPALGYPADGFSLPYQAAISWGSLLVAFLGLWLLRRNLLRHFSDEATAITL
                     LLIVFGTNYLDYSAINGAMTHNYLFTLYAALVAVTASWYERPTLLKALGIGFVVGLAA
                     LTRPTEILTALIPLLWGVANAEDLQKRLGLWKKHGLQMAGAVVVCGLMGSFQLLYWKM
                     ASGDWIVYSYEDQGFSWLKPHIDRGVFSFRAGWLVYTPMMAFAVLGFVWLYKKHAALF
                     PAILVFFLLFLYVTFAWDIWWYGGSLGQRAMVQSYAVLAFPLAAFVEWLKDRKTLRYV
                     VFAIFVLFSYYNLWLTHQAHKGGMLEPGFMTKAYWKRIVLRYDLPVETRKLLDTDRDY
                     GNRRRNVQVIYENNFETAELWADCPIPPIEGSRSACIGGGRQFTEPFGFELPANGSRK
                     LRAVVTVRSLNKEWDTWKMAQLIVRVKNNDEAVHERAIRVFRLLNDNETKEIYLDLRL
                     PDRPFNRAEVFFWNADSDKPLAIDQLRVERFD"
     gene            complement(5404..5814)
                     /locus_tag="PK198_18905"
     CDS             complement(5404..5814)
                     /locus_tag="PK198_18905"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRF40871.1"
                     /translation="MADSVIINKAASIERCLKRIAEDYVGHEAAFETEYIRQDAVLLN
                     LQRACEQSIDLANHLVKLKALGVPQNTREAFILLEKAGLIDAPLARAMEHMVGFRNIA
                     VHEYARLNLSVVRAIIEKHTADFRRFVSIALTFA"
     gene            complement(5807..6190)
                     /locus_tag="PK198_18910"
     CDS             complement(5807..6190)
                     /locus_tag="PK198_18910"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF40872.1"
                     /translation="MNFLRDRLPELRGLWLFGSRADGAYRADSDYDLAFLTDWPSKVS
                     DMQRFEWICALAELLGNEVDLVDLHQVPTDFRFIIVSTGRRIFSSDDYLCDTFEMTSF
                     SMYQYLEEARREIIHEIKKRGSVYG"
BASE COUNT         1454 a         1772 c         1646 g         1394 t
ORIGIN      
        1 tttaaaaaaa tcattccggc gtgtaatttt gcgccccaaa atccggcatt tatgaaaaat
       61 cttgttttgc tgatgttttc tgctgtcctg ctcgtagcgg gctgtgtgaa tgataagacc
      121 tccaaatcgg acagcgaggc tgctgatacg gcggctgtta ccaacgaccc caatgtaaag
      181 tttgtgccgc ccgcgccttt ggtggacatt gacaccagcg ctgcccgcat cggcaacggc
      241 gcttacgatt tcaacgcctt ggtgccgctc ttccccgaca tcattgccgg caccggccgc
      301 caaaccgcca atggggaaaa ttacaaaatg gccggcggca tgatcagcag tgccagcgct
      361 tcttacaatg tgcaggatcg caggttttcc gtcgccattc aggatgtggg cgacaatgcc
      421 gatgtgattc cgcaattggc caagtggtcc actatggcta tgaacggcga cgatgaaaat
      481 gaggtgatgc gctccacctt atacctcaat cagcctgcat ttattcacta taacaaggcc
      541 agcagatcgg gcagctacag cgtcatttat tccaatcgtt ttgtggtgga cattaccggc
      601 agaaacgtag aactggccga gttggaaaag gcgatggagg atttgcgtat agaccgattg
      661 aagtaatttt tcgcgggctg tgttattgcg tttgaaaatt gacgttcttt gtctttttca
      721 aacttaaatt gcacactcac catgaaaaag acaattacca ttttgctctt ttgggcaatg
      781 gccttgccaa tgatcgctca gggcacattg cagggcaaag tcaccgacga aaaaggcgaa
      841 ccgctcatcg gagtagccgt gtacgaacgc ggcaccgact tcggcacttc caccgatctc
      901 gacggcaagt acagcctcac acacagcggc cccaatgcgg tcattacctt tagtatgctg
      961 ggctttgtta cacaggaaat caaggccggc aatcaaaaaa cattggatgt aaagctcctc
     1021 cccgacgtca gcctgctcga tcaggtggtc gtgttgggtt cccggcgcag caaccgggcg
     1081 caaaccgaaa ccatggcccc cgtggacgtc atcaacatac ggcaggctag catgagcacc
     1141 gcccgttttg atgtcacctc catgatgaat tacagcgcgc cctcttttaa ttacaacaaa
     1201 caatccggct ccgacggcgg cgatcatgtg gacttggcta ctttgcgcgg tctcggcccc
     1261 gaccaaacac tcgtgttggt caacggcaaa cgcaggcatc agacggcctt cgtggccgta
     1321 ttcggcaccc gtggcagggg caattccggc accgacctca acgccattcc cgccgcttcg
     1381 gtagagcgca tcgaggtatt gcgcgacggc gcttccgctc aatacggctc ggatgccatt
     1441 gccggcgtgg tcaacatcat tctgcgccgc gacaccggtt tcctcaatgc cgatcttggc
     1501 ttttccgttt atcacgaccc gaaatacaat ccggcctaca aacacgatct gggcctgtat
     1561 gtgcacggca acaaattgga cggcgcggct tacaccgcca atgtcaacta cggcctgcct
     1621 ttgggtaaaa aaggcggttt tgtcaatgta ggcttgcaat acgccgatct ggccaagact
     1681 ttccgacagg aattagacgg cgtgttgccc gaaagcatct accgcagggc gcacggcgac
     1741 ggctccatgc gcgggctggg cggcatgttc aatctggaag cgcctgttaa agaaaacgac
     1801 cgaatgacct tttacgcctt cggtggttac aactcaaaaa actctgacgc ttacgccttt
     1861 acgcgcaatt tctcggctcg gccggagcgc tttcctacca aatccgacgg tgaaatcata
     1921 ccggcgccgg gcatcatttt taccgattcg ggcggcgaac agtactacaa tccacgtatt
     1981 cgcaccgaaa tttccgactt ctccgtggcc gccggtctca aaggcatcag cgcctcgggc
     2041 tggaactggg atttcagcaa tacgctgggg cgcaacaact tccacttctt cggcgaaggc
     2101 actttcaatg caggcttggg gcctgataag acttcctttg acgacggcgg gttcagtttc
     2161 ctgcaaaaca gcgtgaacct caacttcagc aaagaagtgg cctccatttt gcagggcgcc
     2221 aactttgctt tcggtgcaga ggccaggctg gaaaactacc aactctttgc cggcgaagaa
     2281 gcctcgtatc gcaattacga ccccgacaaa gcctccggcg cgcagggctt tccgggctac
     2341 caaccctccg acgaggccaa tgccaaccgc tcctgtatcg gggtgtatgg cgatgtggaa
     2401 ttggacctga ccgatgcctg gttgctgggg ggcgctgttc gtatcgaaaa ctacagcgat
     2461 tttggcttga ctgccaatta caagttgtcg agccggatga gactgtcgcc caaattcaac
     2521 ctgagaggct cggtcagcac cggtttccgc gcgccgtctt tgcagcagat caacttcagc
     2581 tctaccttca ccaccgtgca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggagcgctcg
     2701 ttcaacgcca atctgggttt cacgttcaag cctgtgcgcg agttttccat caccttggat
     2761 gcctatcagg tgaaagtgaa agaccgcgtg gtgctttccg gccagtttga tgcagaagac
     2821 ggcaccttga acccagtatt tacggcagct ttgcagaatc tgcgcgtcag tctggcgcag
     2881 ttttttgcca atgcggtgaa caccaccaat cgcggtattg acatcgtgtt ggattacaac
     2941 aagcgccgct ccaacggcca tttcagagtg ctggctgccg ccaatttcca gcaaatgacg
     3001 attgaccaaa tcaacatgcc cactttgctc aacgacacgc cggagcaccg cgctaccttc
     3061 ctcagcgacc gcgaacaagc ctttattctg gcttccgctc cgcctgttaa aatcaacggt
     3121 acggtggagt acggcattga gaaatttacg ctgggtgcgc gcctcaacta cttcggcaag
     3181 attgaattgc tgggctatgg cgaagacggt ctgggcatca atccgatggt gcccacggat
     3241 gccgacgaga atatttatgt ggaagaccgc tacatctatg gcggcaagtt ggtacccgat
     3301 ctttacatcg gctggcaggc gtccaagagc atttccctga atttcggcgt ggacaatatc
     3361 ctcaacgttc accccgatct gggctttgta ccggtagctt ccggctgggc tttcaacaac
     3421 gaaacgggcg gcccttggga tgcggtgcaa atgggcggca acgggcgccg gttctttgtg
     3481 cggctgggct tgaattttta gaattgaatt tcccgccact ttgattcctg cgggatttgt
     3541 ttttaaacac aataggcaca tagatcacat agcgcagttg ctatggttct gtgtgcctat
     3601 atcaatcaaa ccgctccacc ctcaactgat ctatcgccag aggcttgtcg ctgtcggcat
     3661 tccagaaaaa cacttctgcc cggttgaagg gccggtccgg cagccgcaga tccaaataaa
     3721 tctccttcgt ttcgttgtcg ttcagcagcc tgaacacccg aatggcgcgc tcatgcaccg
     3781 cctcgtcgtt gtttttgacg cgcacaataa gctgggccat cttccaggtg tcccactctt
     3841 tgttgagcga gcgcaccgtg acgacagccc gcagtttgcg cgaaccattg gccggcaatt
     3901 caaacccaaa cggctcggta aactgccgtc ccccgccgat acaggccgag cggctcccct
     3961 cgatgggcgg aatggggcaa tcggcccaga gttcggcggt ttcaaaattg ttttcgtaaa
     4021 tcacctgcac attgcggcgg cgattgccgt agtcgcggtc ggtatcgagc agtttgcgcg
     4081 tttccaccgg cagatcgtag cgcagcacaa tgcgtttcca gtaggctttg gtcataaaac
     4141 ccggctccag cataccgccc ttgtgcgctt gatgcgtgag ccagagattg taataactga
     4201 acagcacgaa aatggcaaaa acaacgtagc gcaaggtctt gcggtctttg agccactcca
     4261 caaaggccgc cagcggaaag gccagcaccg catagctttg caccatggcg cgttggccca
     4321 gactgccgcc gtaccaccag atgtcccagg cgaaagtgac gtacaaaaac agcaggaaaa
     4381 acacgagaat ggccggaaac aaagcggcgt gctttttgta caaccacaca aatcccagca
     4441 cggcaaatgc catcatgggc gtataaacca accagcccgc ccggaaactg aacacgcccc
     4501 ggtctatatg cggtttcagc cagctaaagc cttgatcttc atagctatac acgatccagt
     4561 cgccgcttgc cattttccaa tacaacagtt gaaagctgcc catcagtccg caaactacaa
     4621 ccgcgccggc catttgcagc ccatgttttt tccacaggcc gaggcgcttc tgcaaatcct
     4681 cggcattggc cacgccccag agcagcggga tgagcgccgt caggatttcg gtaggtcgcg
     4741 tgagggccgc caagccaacg acaaaaccga tgcctaaggc tttgagcagg gtagggcgtt
     4801 cgtaccacga ggccgtcacc gccaccaatg ccgcgtagag ggtgaagagg tagttgtggg
     4861 tcatggcgcc gttgatggcg gagtagtcta agtaattggt tccgaacaca atgagcagca
     4921 gcgtgatggc cgtagcctcg tcgctgaaat ggcgcaacag gttgcggcgc agcagccaca
     4981 aacccagaaa tgccaccaac aaactccccc aactgatggc cgcctgatac ggcagcgaga
     5041 agccgtcggc ggggtagccc aacgccggcg ccagtgcatg cgcgatgaga aaaaagggca
     5101 ggtattgcac cgccagaccg caggagtatt tcatcacata attgcccgat gggtgctgaa
     5161 atgcctgata gggcgcatcg gcggggcggt atttctggtg gatggaatcg cggaaagcca
     5221 cctgatggat gtctttgtaa atgaacacgg ccggcaggta aaaataatac cccgacacgt
     5281 cccaactgat ggtggcctcg gtttggccct tgttccactt gggataataa atagcgccga
     5341 ctgcgagcaa aaaagccatg cagatgagca acgaatagag ggagatgcgc ttgttcataa
     5401 ttttcaggca aaagtgaggg cgatggagac gaaacgtcgg aaatcagcgg tgtgcttttc
     5461 gatgatggcg cgcacaacgc tcagattcag ccgggcatac tcgtgtaccg caatgttccg
     5521 aaaccctacc atgtgttcca ttgcacgggc aagcggtgcg tctatcaggc cggctttttc
     5581 caacaaaata aaggcttcgc gggtgttctg cggcacaccc agcgctttca gttttacaag
     5641 gtggttggcc aaatctatac tttgttcgca ggcgcgttgc aaattcagca atacagcatc
     5701 ctgccggatg tattctgttt caaaagccgc ctcatgcccg acatagtcct cggcaatgcg
     5761 tttcaggcag cgctctatgc tcgccgcttt gttgatgatt acgctgtcag ccatacacgc
     5821 tcccccgttt ttttatttcg tgaatgattt cccggcgcgc ctcctccaag tactggtaca
     5881 tcgaaaaact cgtcatttca aaagtatcgc acaggtagtc gtcgctgctg aaaatgcgcc
     5941 ttccggtaga aacgatgatg aatcgaaaat cggtgggcac ctggtgcaag tccaccaaat
     6001 ccacctcatt gcccagtaat tccgccaggg cgcagatcca ttcaaaccgc tgcatatcag
     6061 atacttttga cggccaatcg gtcaaaaaag ccaggtcgta gtcgctgtcc gcacggtagg
     6121 cgccgtcggc tctgcttcca aacagccaca gcccgcgcaa ttccggtagc cggtcccgca
     6181 ggaaattcac gatttgcagc tcggtcgctt gattcatacc ggcaaaggta aaaatagtcg
     6241 tgaaaatttt cagggcgccc ccgccactcg cagtctcgta gttgcagggt agtcaaatgg
     6301 cgggcgggtc atccgctttt ataccgcagg ggctctctgc tctgtgctct ttg
//
LOCUS       DAORLF010000286         6350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6636_length_6350_cov_1.637967, whole genome shotgun sequence.
ACCESSION   DAORLF010000286 DAORLF010000000
VERSION     DAORLF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_6350_cov_1.637967"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK198_18915"
     CDS             <1..262
                     /locus_tag="PK198_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRF40873.1"
                     /translation="DKDEVYFTPLYYTLAHFSRFIRPGAVRIGFNHTDNNLMVTAARN
                     PDGSIAVVVFNPEAQSKSLRLKLAKQTYDFSIAAKAVVVRGG"
     gene            358..>2358
                     /locus_tag="PK198_18920"
     CDS             358..>2358
                     /locus_tag="PK198_18920"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF40874.1"
                     /translation="MSANSTVNTHKVPFGQKVAFGLGMLANQMFPAALGIFMVVLVQD
                     LGFPGWMWSAIYFFPRIFDSLTDPIMGYISDNTRSVWGRRRHYVFIGAIVMGIAFVTM
                     WQLYRENGVEYNFYYFMAWSFIFYLGLTLFSVPYVAMGYEMSDDFHERTNIMAIAQWI
                     GQWAWVIAPWFWVIMYDQGWFPNADTATRSLALWVGIACALLAMVPAIFIKSKSTKDD
                     DSLEPLTLRGMGGSLMQIFDGFKQAFQSVPFRKLCISTFLIFNAFNTVAAFSFFIVVY
                     HLFDGDAGAAGVWPTLFGCLGALFTTFLVIPTVAWLSKKLGKKRAFLLSQGISVFGYI
                     LLWLLFIPGKPYMFIFALPFFSFGIGSLFTLMMSMTADVCDLDELNSGKRREGIFGAI
                     YWWMVKFGFAIAGGLSGLIISYVGLTPGAAEQPEGAVTGLRLFFSGVPMLGTLIAMFV
                     MRNYDLDEARAREIQAELARRKAPQASGYAKSGMGWEGLSRSEILARFPHFFQGASDV
                     SRLSGKELRGQFGQRLESGMHGMCFSAYQEGQNPWGDTITEEQVRRRLEVVRPYTQWI
                     RTFSCTNGHERIPEIAKEMGFKTMTGAWISNNKEQNELELASLIQLINKGVVDIAAVG
                     NEVLYRGELSEEAVCEYVRRVKQSSGNIPVTYVDAYYQFINRP"
     assembly_gap    2359..2368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2369..2721
                     /locus_tag="PK198_18925"
     CDS             <2369..2721
                     /locus_tag="PK198_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRF40875.1"
                     /translation="ACDVIPINCYPFWEGAGIETAGLYLQEMYRKTKEAAKGKPVIIA
                     ETGWPSKGAPVDDALPSEENYMRYFLDAQTWSEHQQVPCFHFSSFDESWKIHAEGWAG
                     TSWGIWDVKERRKF"
     gene            2904..3836
                     /locus_tag="PK198_18930"
     CDS             2904..3836
                     /locus_tag="PK198_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRF40876.1"
                     /translation="MSYRKEKVLSAEQLTQMLSHAGAAYPGELTLEDINHLFLEVFHN
                     GVHGLCFSLYEDGQRPGDVISEEQVRRRMEIIKPYTQWVRSFSCIEGNEFIPKVAKEM
                     GIKTLVGAWLGDDLEKNEQEIEALIELARKGWVDIAAVGNEVLYRKDLTEAELLAYID
                     RVKKQLPAGIPVGYVDAYYEFSHRPAITAACDVILSNCYPYWEGCPFEYSLGHMHQMY
                     DQALDAAQGKKVIITETGWPSQGESLKGAHPSYENAIKYFINTQLWAGDHDVEVFYFS
                     SFDESWKVGAEGEVGAYWGIWDKSEKLKFGNGHI"
     gene            3823..>4116
                     /locus_tag="PK198_18935"
     CDS             3823..>4116
                     /locus_tag="PK198_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40877.1"
                     /translation="MDIFDQLGIPSGRAICYSGFRAGQHPGGKHPDYEEIKEDLLLLQ
                     DHWKYLRLYDCDPHAETVLQVIRQEKLDFKIMLGAFIEAEMNNFNCPWGGGVYS"
     assembly_gap    4117..4126
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4127..>4493
                     /locus_tag="PK198_18940"
     CDS             <4127..>4493
                     /locus_tag="PK198_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40878.1"
                     /translation="DRKKNTSTGKSAGNQRKINRLIAWGRQYPDIVFSLSVGNEACVD
                     WTDHYVSENQVLEYARQVKSQAVQPVTFCENYVPWLYKLQPLAEALDFISIHTYPVWE
                     YKHIDESLDYTKENFYAVAR"
     assembly_gap    4494..4703
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4917..5077
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5078..6165
                     /locus_tag="PK198_18945"
     CDS             <5078..6165
                     /locus_tag="PK198_18945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40879.1"
                     /translation="SMHGADLGPFVLGPSNSIIKIMVWKSVISDVGIKMASPAGWAQV
                     EIKRPNTVVNQWEELTFDFSNYVNPPADLGQLDQIIVFPDFNLAGRGQNNIIYFDNIT
                     FGPAGGGNPTVPMVAAPTPTRPASSVISLFSNAYTNVPVDTWRTGWSNAILEDIAIQG
                     NATKKYSQLDFVGIETVANQIDATGMTHIHIDVWSADFTFFGFKLVDFGADGAFGGGD
                     DVEHQVNYNLPQQGQWLSLDIPLSSFAGLTTRAHMAQYILVGQPTGANTIYVDNVYFY
                     TNIVDTDEAEAFRNTVRVYPNPVAAGEQVQFSREVSQIEVFDLAGQRVLAGSGFRLNT
                     AVLNPGVYVLKMRVQDGSIQTQKLVVK"
BASE COUNT         1432 a         1467 c         1593 g         1467 t
ORIGIN      
        1 tgacaaagac gaggtgtatt tcacgccgct gtattatacg ctcgcccatt tcagccgctt
       61 catccggccc ggcgccgtca ggatcggatt caaccataca gacaacaacc tgatggtaac
      121 agcagcgcga aatccggacg ggagcattgc cgtagtagtc ttcaatccgg aggctcaatc
      181 caagagttta cgcctgaagt tggccaaaca aacttatgat ttttccattg cagccaaggc
      241 tgtggttgtt cgaggcgggt gagtgaaggg ggggaatcgc agtcactttc actaagcaat
      301 gtcaatcaac aagttatcac gccctaattt tttcaaatca ttaataaaac caacatcatg
      361 tcagcaaaca gtacggtaaa cacacacaaa gtccccttcg gccagaaagt agctttcggc
      421 ctcggcatgt tggccaacca gatgtttccg gcggcgctgg ggatatttat ggtcgtgctg
      481 gtgcaggatt tgggattccc cggatggatg tggagcgcca tctacttttt ccccagaata
      541 ttcgactccc ttaccgaccc catcatgggc tatatctccg acaatacacg ctcggtgtgg
      601 ggacggcggc ggcattatgt ttttatcggc gccattgtca tgggcattgc ctttgtgacc
      661 atgtggcagt tatatcgcga aaacggggtt gagtacaact tttactattt catggcttgg
      721 tcgtttattt tttacctggg ccttactctt ttcagtgtac cttatgtagc tatggggtat
      781 gaaatgagcg acgattttca tgaacgcacc aatattatgg ccattgctca atggataggg
      841 caatgggcct gggtgattgc gccctggttt tgggttatta tgtacgatca ggggtggttc
      901 cccaatgccg ataccgccac ccgtagtctg gcgctttggg tgggcatagc ttgcgcactg
      961 ttggcaatgg tgccggctat ttttatcaaa agcaagtcca ccaaagatga cgacagcctc
     1021 gagccgctta ccctacgcgg gatgggtggc agcctgatgc aaatattcga tggcttcaag
     1081 caagccttcc agtctgtgcc ttttcggaag ttgtgcattt ctacctttct cattttcaat
     1141 gcattcaata cggtagctgc cttctctttc ttcatcgtag tgtatcacct gttcgatgga
     1201 gatgccgggg ctgcgggtgt atggcctact ttgtttggtt gtttgggggc tttgtttact
     1261 acctttttgg tcattcccac ggtggcctgg ttgtcaaaaa aattgggtaa aaaacgcgcc
     1321 tttttgctct cgcagggcat ttcggtgttt ggctacatac tgctttggct gctgtttata
     1381 cccggcaaac cgtacatgtt catttttgcc ctgccgtttt tctccttcgg catcggcagc
     1441 ttgttcaccc tgatgatgtc catgaccgcc gatgtatgtg atttggatga actcaacagc
     1501 ggcaagcgcc gggagggcat cttcggtgct atttactggt ggatggtcaa gttcggtttt
     1561 gccattgcag gcggcctcag cggtttgatc atttcttatg tgggattgac acccggcgca
     1621 gcggaacaac cggagggcgc ggtgacaggc ctgcgtctgt tcttttctgg cgtgcctatg
     1681 cttggcaccc tcattgccat gttcgtcatg cgcaattacg acttagacga ggcgcgtgcg
     1741 cgcgaaattc aggcggaatt ggcccgcaga aaagcgcccc aggcttcggg ttatgccaag
     1801 tcgggcatgg gctgggaagg gttgtcccgg tcggaaatcc tggcgcgttt cccgcacttt
     1861 ttccaaggtg catcagatgt gagccgtctg agcggcaaag aacttcgggg ccaattcggg
     1921 cagcgtttgg aaagtggtat gcacggaatg tgtttcagcg cctatcagga aggacaaaac
     1981 ccctggggag ataccattac cgaggagcag gtgcggaggc gattggaggt agtgcggcct
     2041 tatacccaat ggatacgcac gttctcctgc accaacggcc atgaacggat tccggaaata
     2101 gccaaagaaa tgggctttaa aaccatgacg ggcgcctgga tcagtaacaa caaggaacag
     2161 aacgaactgg aactggcctc gctcatacaa ctcataaaca aaggagtagt ggacattgcc
     2221 gcggtgggca atgaagtgct gtatcggggt gagttgtcgg aagaggctgt gtgtgagtat
     2281 gttcggcgtg tgaaacagag ttcaggcaac ataccggtga cttatgtgga cgcatattat
     2341 cagttcatca accggcccnn nnnnnnnnag gcttgcgatg tcattcccat caactgctac
     2401 ccgttctggg agggcgcggg cattgaaacc gccggattgt accttcagga aatgtatcgc
     2461 aagaccaaag aagctgccaa aggcaagccg gtcatcattg cggaaacggg ctggccgagc
     2521 aagggcgcgc cggtggacga tgccttgccc tcggaggaaa actacatgcg ctacttcctc
     2581 gatgcgcaaa cctggtcgga acatcagcag gtaccctgtt tccatttctc ctctttcgat
     2641 gaatcctgga agatacatgc agagggctgg gcaggtacgt cctggggtat ttgggatgtg
     2701 aaggagcgtc gtaagttttg atgaagagga aatcaagccg gcagctatta gccattagcc
     2761 actggctgtt agatggataa tccgtccata gttgatgatc ctttcgggga gtcttaaata
     2821 aagggtgacc aaccaaattt tcgaaaaatc aagctaaaag ctagaggcca acagctaata
     2881 gctaagacca aaaatcaaga gccatgtcat acagaaaaga aaaagtctta tcagcagagc
     2941 aactgaccca aatgctgagc catgccggag cggcctatcc cggcgaactc acattagagg
     3001 acatcaacca cttgtttttg gaagtttttc acaatggcgt tcacggcctg tgcttcagtt
     3061 tgtatgagga tggacagcgc cccggcgatg tgatttcgga agagcaggtg cgtcgccgca
     3121 tggaaatcat aaagccctac acacaatggg tgcgctcgtt ttcctgcatt gagggcaatg
     3181 agtttattcc caaggtagcc aaagagatgg gcatcaagac tttagtaggc gcctggttgg
     3241 gcgacgacct cgaaaagaac gagcaggaaa tagaggcgct cattgagttg gcacgcaaag
     3301 gttgggtgga tattgccgca gtgggcaatg aagttcttta ccgcaaagac cttaccgaag
     3361 ccgaactact ggcttacata gaccgcgtca aaaagcaact gcccgccggc attccggttg
     3421 gctatgtgga cgcctattac gaattcagcc accgcccggc cattacggca gcctgcgatg
     3481 tgattctgag caattgctac ccctattggg agggttgccc gtttgagtac tcactgggtc
     3541 acatgcacca aatgtacgat caggcgctgg atgccgctca gggcaaaaaa gtcatcatca
     3601 ccgaaaccgg ctggcccagt cagggcgaaa gtctcaaggg ggcgcatccg tcttacgaaa
     3661 acgccatcaa atacttcatc aatacccagc tttgggccgg tgatcacgat gtggaggtgt
     3721 tttacttctc ctcttttgac gagtcttgga aagtgggcgc cgagggcgaa gtgggcgctt
     3781 actggggcat ctgggataaa tcggaaaaac tgaaattcgg caatggacat atttgatcaa
     3841 ttgggcatac catccggcag agccatctgt tattccgggt tcagagcagg gcagcatcca
     3901 ggcggaaagc atcctgatta cgaggagatt aaagaggatt tgctgttgtt gcaagatcac
     3961 tggaaatacc tccggctgta cgattgcgac ccccatgccg aaaccgtttt acaagtcatc
     4021 cggcaggaaa aattagattt taaaatcatg ctgggcgcct tcatcgaggc cgagatgaac
     4081 aacttcaatt gtccctgggg aggtggcgtg tattccnnnn nnnnnnagat cgcaaaaaga
     4141 acacgagcac aggcaaatct gccggcaatc aacgcaaaat caaccggctg atagcatggg
     4201 gccgccaata tcccgacatt gtgttttcgc tttcagtggg caatgaagcc tgcgtggact
     4261 ggactgatca ctatgtgtct gaaaatcagg tgttggagta tgcccggcag gtgaaatccc
     4321 aggccgttca gcccgtcacc ttttgtgaaa actatgtgcc gtggttgtat aagttgcagc
     4381 cattggccga ggcgctcgat tttatttcca ttcacaccta tcctgtgtgg gagtacaaac
     4441 acattgacga gtcattggat tataccaaag agaatttcta tgccgtagcg cgcnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnccagatc ctatggagcc tgaaaaacat tggggactgt
     4741 tcaaaattga ccggactccg aaattggcgc tcacacactt gtttcaatct gctgacttat
     4801 cttacaacca ataattcact aaatgttctg attatgaaga atcttacatt ctatttcctg
     4861 atgttgctgg cggcaacggg ctttgcccag aattcgccca tcaattttga ggccggnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat ccatgcacgg cgccgatctg
     5101 gggccattcg ttctggggcc ttccaattcc atcatcaaaa tcatggtttg gaaatcggtc
     5161 atcagcgatg tgggcatcaa aatggcctcc cccgccggat gggcgcaagt ggaaatcaaa
     5221 cggcccaaca ccgtggtgaa ccagtgggaa gagctgactt tcgatttctc taattacgtc
     5281 aacccgcctg ccgacctggg ccaattggac caaatcatcg ttttccccga cttcaacctc
     5341 gccgggagag gacaaaacaa catcatctat ttcgacaaca tcacctttgg tccggcggga
     5401 ggaggtaacc ctactgtgcc gatggtagct gcgcccacgc ccacccgccc ggcatcgagc
     5461 gtgatctccc tgttcagcaa cgcttatacc aacgtaccgg tggatacctg gcgcacgggc
     5521 tggtcgaatg ccattttgga ggacatcgcc attcagggca acgccaccaa aaaatacagc
     5581 caactcgact ttgtaggcat cgaaaccgtt gccaatcaga tagatgcaac aggtatgacg
     5641 cacattcaca ttgacgtatg gtcggcagat ttcaccttct ttggtttcaa attggtggac
     5701 ttcggagcag acggcgcttt cggtggcggc gacgatgtag agcatcaggt caactacaat
     5761 ttgccgcagc agggccaatg gctgagcttg gacattccgc tctcttcctt cgccggcctc
     5821 accacccgcg ctcacatggc acagtacatt ctggtaggac agcccacggg cgcgaacacc
     5881 atttatgtgg ataatgtgta tttctatacc aacatcgtgg acacagacga ggccgaagcg
     5941 tttagaaata cagtaagggt atatcccaac ccggtggctg ccggcgagca ggttcagttc
     6001 agccgggagg tatctcaaat agaggtgttt gatcttgcgg gccaacgcgt cttggcgggc
     6061 agtggtttca ggctcaacac ggccgttttg aatccgggag tatatgtgct caagatgcgc
     6121 gttcaggacg gaagcataca aacacaaaaa ctcgtggtga agtaaggctt ctccgcattg
     6181 gggagtgttt tggctgaggt atttttttat atccagccaa aacctctttt ttttcgttgt
     6241 tttggctggg ttaactttta tacctcgcac cgtcaggtgt gaagccgctt ttgtcatacc
     6301 gggcgctgct aatcaacaac ttgcgccgaa ggctatgcac aaaacctggc
//
LOCUS       DAORLF010000287         6341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6652_length_6341_cov_1.986001, whole genome shotgun sequence.
ACCESSION   DAORLF010000287 DAORLF010000000
VERSION     DAORLF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_6341_cov_1.986001"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PK198_18950"
     CDS             <1..663
                     /locus_tag="PK198_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF40880.1"
                     /translation="IQVNEQLYLRDPQDSKLGRNILQHGICLIEELGLEEFTFKKLAH
                     RIESTEASIYRYFENKHKFLVYLLCWYWERVRYQITLATTNIETPEQKLRLALSAIVD
                     SSKADPAVEYINEHILFRIVLTEGPKTYHTKAVDEENKKGFFAVYKALAADLAAIIGE
                     VNPAFEYPKALASTLLEMANFHIYAAQHLPSLTEVRTPADDLTEVKNMLTFFAFGLLD
                     RE"
     gene            complement(666..1319)
                     /locus_tag="PK198_18955"
     CDS             complement(666..1319)
                     /locus_tag="PK198_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF40881.1"
                     /translation="MMTEEQQIREYLRKYYSNIAELGLQEEIAHIGKVMEFKAGEIIM
                     DFGAYVKLIPLVVEGSIKVSREGDDGGEIFLYYLQPGESCTMSFTCCMMNKRSEIRTQ
                     AEEDTVVIAIPIRYMDDWMSRYQSWKNFVMMSYDHRMKELIRTIDEIAFKKMDERLLT
                     YLQKKAQVTGSKTLNATHQEIAYDLNASREAVSRLLKQLEHDGYVKLGRNKITLAKG"
     assembly_gap    1394..1415
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1416..1731)
                     /locus_tag="PK198_18960"
     CDS             complement(<1416..1731)
                     /locus_tag="PK198_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRF40882.1"
                     /translation="MSLKKKRPVMANMLAMKCPRCQEGDLFDTPTFSFKKPFDMPEKC
                     PHCEQDYMPEPGFYYGAMFISYIFMGWFCLLFVMFFHWVLGWGMAASFGLLIAVCIVL
                     FVY"
     gene            complement(1738..2889)
                     /locus_tag="PK198_18965"
     CDS             complement(1738..2889)
                     /locus_tag="PK198_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40883.1"
                     /translation="MEIYSIGQNMYFCGVMEYKDNLLGVVGTLYRWRKPLIALCLLAG
                     LGTAGISLMLPDYYQAGTTFFAASPDQSKPELVFNRSMSMRMEYYGNSGDIDRLLTVA
                     ESSELVGFLVDSFDLFDHYEIDPNQHKAPFLMQERFFSLYTVKKTKRDAIELTVEDQD
                     PQLAAGIANAARNKIEEIVQRLIRESQQKTIKAYEDNIAAKEAFLLQLGDSLAVLRTQ
                     YGTYNVVAQTETLTEQLSEALALFTRDSVRLLVLRANAQVPRDTIYMLDAKVAGLRQE
                     VKVLKGEISRLNSGISTIYNLEKQFAEANQILGEDRERLKQWRAANTSTAPAVLLVEA
                     ASPPVIKSRPKRSILVLSATFVVFLFGVIGILLFDAYKSINWREVLGSK"
     gene            2913..>3416
                     /locus_tag="PK198_18970"
     CDS             2913..>3416
                     /locus_tag="PK198_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40884.1"
                     /translation="MATWITKLFRWILPEDPSYRRYSSGRPALVVPFRGFVSQGRAYL
                     HGRVLRDRRIQVSPGDSLWRNLGNNYKRFGSLTIPNALIHITIGKNKLHLVSDNYGYF
                     HYDGPLQYPLEPGNDAWTDFSLHLLGTLGEIFDYQCSGEVLAPCNARFGIVSDVDDTI
                     VGNAPPID"
     assembly_gap    3417..3454
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3455..3997
                     /locus_tag="PK198_18975"
     CDS             <3455..3997
                     /locus_tag="PK198_18975"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HRF40885.1"
                     /translation="LVKNAFGRQAFQSVADFYQALQKGPDGTCDNPVFYVSNGPWNLY
                     DLLCDFFQLHELPKGPVFMRDFGFLGRRLWGKPSTHKADHIRRIITAYPDLPFVLIGD
                     SGERDTDIYMEIARSFPGRILAIFIRDVEIPGRALRISRLIESAQGEIVMHIIKNYRE
                     AALFARQLGLIEPLPPDEFT"
     gene            complement(3994..>5490)
                     /locus_tag="PK198_18980"
     CDS             complement(3994..>5490)
                     /locus_tag="PK198_18980"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF40886.1"
                     /translation="VFRIYADKNNRPAAYSADNVPYRPKHFLPISLGGIKEGDFSLTY
                     GFPGRTNLYLPASAVEMQVNVLNPIRTGIRDQILGIIGAAMRADAEVKLQYTSKQAGI
                     SNAWKKWIGETQGLKRVNGLEQKRRMEAEFKRRAANAALNAKYGQILPELDRLYAELT
                     PLGIHRDHFNESTRQIELFRLASMVDGALRFKETQGAEAAQKRSAQFIGAMKNYYKDY
                     RVEVDRQLFELLIGIYFDKVPAAYQPQYAIDLYNKHGRSAAKLTETIYASTMLSSPNN
                     GLALLETNWDKGLEELQNDPALKLYREIINTGNDKIMRPATEIQERITPLQRAYMQAL
                     IDVFPEKKMAPDANSTLRVSYGKVMGYRPRDAVRYEPMTYLDGVMEKYKPGDYEFDVP
                     EKLRSLYAAKDYGQYADNGRMPVCFISTSHTTGGNSGSPTFDARGNLVGLLFDGAWEG
                     VVSDYYFSDEINRSIIVDARYILFVIDKFGGAGHLVNEMKLVPGKGKR"
     assembly_gap    5491..5588
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5589..6140)
                     /locus_tag="PK198_18985"
     CDS             complement(<5589..6140)
                     /locus_tag="PK198_18985"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF40887.1"
                     /translation="MLTIISTGLRADEGMWLPLLLREMNEPQMKKLGMKMSAEDVYSI
                     NKGSLKDAVVSFGGGCTGGVVSAQGLLLTNHHCGHGRIQALSTPEKNYLEDGFWAKSL
                     SEELPSQGLFVTFIVRIEEVTQAVLAGVTAGAPEKERQSAIDKNLETVRGQAQKESWQ
                     SAFIRPFYEGNRYFLFVTETFNDI"
     gene            6151..>6341
                     /locus_tag="PK198_18990"
     CDS             6151..>6341
                     /locus_tag="PK198_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40888.1"
                     /translation="MIIFLIMLFFVFRKKRQVRNVTAIFQQHKSPDENSRHFFHLAPH
                     PLTHSPLTPHPLTPHLLTPV"
BASE COUNT         1385 a         1717 c         1562 g         1519 t
ORIGIN      
        1 attcaggtca acgagcaatt gtatctacgg gacccgcagg acagcaagct ggggcgcaac
       61 atcctgcaac acggtatttg cctgatagag gaattggggt tggaggaatt taccttcaaa
      121 aaattagcac accgaatcga atccaccgag gcttccattt atcgctattt tgaaaacaag
      181 cacaaatttt tggtgtattt gctatgctgg tactgggaac gcgtgcggta tcagatcacg
      241 ctggccacca ccaacatcga aacccccgaa caaaaactgc gcctcgccct gtcggccatt
      301 gtggacagca gcaaagccga tccggctgtg gagtatatca atgagcatat cttattcaga
      361 attgtactga cggaaggccc caaaacctac cacaccaaag cggtggacga ggagaacaaa
      421 aagggattct ttgccgtgta taaagcattg gccgccgacc tggcagccat tataggggaa
      481 gtcaaccccg cctttgagta cccgaaagca ttggccagca ccttgctgga aatggccaat
      541 tttcacattt atgcagcaca gcacctgccc tcgctcacgg aagtgcgcac gcccgcagac
      601 gatctgacgg aggtgaaaaa catgctgacg ttttttgcct ttggcttact tgacagggaa
      661 taaaactacc ctttggccaa agtgattttg ttgcgcccca gtttcacata gccgtcgtgt
      721 tccaactgtt tgagcagccg agacaccgct tcccgcgagg cgttgaggtc ataagcaatt
      781 tcctgatggg tggcattcag cgttttggaa ccggtcacct gcgctttttt ctgcaaatag
      841 gtgagcaggc gctcgtccat ttttttgaac gcaatttcgt ctatggttcg gatcagttct
      901 ttcatgcgat gatcgtaaga catcatcaca aaatttttcc agctttggta cctcgacatc
      961 cagtcgtcca tgtatcgaat gggaatggca atgacgaccg tatcttcctc tgcctgcgtg
     1021 cggatttcgc tgcgcttgtt catcatgcag caggtgaaag acatggtaca cgactcgccc
     1081 ggctgcaggt agtacagaaa aatctcgccg ccgtcgtcac cttcccgcga caccttgatg
     1141 ctgccttcca ccactaaagg aatgagcttg acataagccc cgaagtccat gatgatttcg
     1201 ccggctttga actccatcac cttgccgata tgcgctatct cttcctgtag cccgagttcg
     1261 gcaatgttgg aataatactt gcgcaggtac tctctgatct gttgctcttc ggtcatcatg
     1321 cgtgcttgtg tttagctttg gaaggatcat atttcactac aatattgatc cagatggagc
     1381 gtgcgaggcg aaannnnnnn nnnnnnnnnn nnnnntatac acaaataaaa caatgcacac
     1441 cgcgatgagc agcccaaacg acgcggccat gccccagccc aatacccagt gaaaaaacat
     1501 cacaaaaagc aggcagaacc atcccataaa aatataggaa atgaacatgg ctccgtagta
     1561 aaaccctggc tccggcatat agtcctgctc gcaatgcggg catttttccg gcatatcgaa
     1621 cggctttttg aaggaaaacg tcggcgtatc aaacaggtcg ccctcctgac aacgaggaca
     1681 tttcatagcc aacatattgg ccatcaccgg tcttttcttt tttaaactca tgtcacctta
     1741 ttttgaaccc agcacctccc gccagttgat gcttttgtac gcatcgaaca gcaggatgcc
     1801 gatcacacca aacaaaaata ctacgaaggt ggccgaaagc accaggatac tgcgcttcgg
     1861 cctcgatttg atgaccggcg gcgaagcagc ctccaccagc aatacagcag gcgcagtaga
     1921 ggtattggct gccctccatt gcttgaggcg ttcgcggtct tcgcccaata tctgattggc
     1981 ttcggcaaac tgtttttcta agttgtaaat ggtagaaatg ccgctgttga gccgactgat
     2041 ttcgcctttc aacactttga cctcctgcct caaaccggct actttggcgt ccagcatata
     2101 aatggtatcg cggggcacct gagcgttggc ccgcagcacc agaagccgaa cgctgtcgcg
     2161 ggtgaacagg gccagtgctt ccgacaactg ctcggtgagc gtttcggttt gggctaccac
     2221 gttgtaagtg ccgtattggg tgcgcaaaac cgctaaggaa tcgccaagtt gaagtagaaa
     2281 agcctccttg gcggcaatat tatcctcata agccttgatg gtcttttgct ggctttcgcg
     2341 aatgaggcgc tgtacgattt cttctatttt attgcgggcg gcattggcta tgccggcggc
     2401 caattggggg tcctggtctt ctaccgtcaa ctcgatggcg tcccgttttg tctttttgac
     2461 ggtgtataaa ctgaagaaac gctcctgcat cagaaacgga gctttgtgct ggttggggtc
     2521 tatctcgtag tgatcgaaaa ggtcgaaaga atccaccaaa aagccgacca attcgcttga
     2581 ctcggccacg gtgagcaggc ggtctatatc accggagttg ccgtaatatt ccatccgcat
     2641 agacatgctg cggttgaaca ccaattccgg cttgctctga tcggggctgg ctgcaaaaaa
     2701 tgtagtaccg gcctggtagt aatccggcag catcaacgaa atgcccgctg tgcccaaacc
     2761 ggccagcaaa cacagtgcta tgaggggctt tctccaccgg tacagcgtgc ccactacgcc
     2821 caataaattg tctttgtatt ccatgacgcc gcaaaagtac atattttgcc cgatgctgta
     2881 tatctccatg aattgcataa ctttacccac gcatggctac ctggattaca aaactcttcc
     2941 gctggatatt gcccgaagac ccgtcttacc ggcgatacag ctccggacga cctgctctgg
     3001 tggtcccttt tcgcggcttt gtttcgcagg ggcgggcata tctgcatggc cgggtactgc
     3061 gcgaccgccg cattcaggtc agccccggcg attcgctgtg gcgcaatctg ggcaacaatt
     3121 acaaacgatt cggcagcctc accattccca atgcgctcat tcacatcacc ataggcaaaa
     3181 acaaactgca tttggtgagc gacaactatg gctactttca ttacgacggc ccgctgcaat
     3241 accctttgga gccgggcaac gatgcctgga ccgatttttc gctgcatctg ctgggcacgc
     3301 tgggcgaaat ttttgattac caatgttcgg gagaggtgct ggcgccctgc aatgcccgct
     3361 tcggcatcgt cagcgatgtg gacgacacca tcgtcggcaa cgcgccgccc atcgacnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttggtg aaaaacgcct ttggccgcca
     3481 ggcttttcag tcggtagccg atttttacca agccctgcaa aaaggccccg acggcacttg
     3541 tgacaatccg gttttttacg tttccaacgg cccctggaac ctctacgacc tgctttgcga
     3601 tttttttcaa ctgcatgagc tgcccaaagg ccctgtgttc atgcgggatt ttggctttct
     3661 gggccgtcgc ctctggggca aaccctctac tcacaaggcc gatcacatcc gccgcatcat
     3721 cacggcatac cccgacctgc ccttcgtact catcggcgac agcggcgaac gggatactga
     3781 tatttacatg gaaatagcgc gcagttttcc ggggcgcatt ctggccattt ttatccgcga
     3841 tgtggaaatt ccggggcgcg cccttcgcat cagccgtctc atcgaatcgg cacagggaga
     3901 aatcgtcatg cacatcatta aaaactaccg ggaggcagcg ctcttcgccc gacagttggg
     3961 gctgatagag ccgctgccgc ccgatgaatt cacttaacgc ttgcccttgc ccggcacgag
     4021 tttcatctcg ttgaccaagt ggccggcgcc gccgaatttg tctatcacaa acaagatgta
     4081 gcgcgcatcc acaatgatgc tgcggttgat ctcatcgctg aaatagtaat cgctgacgac
     4141 tccttcccag gcgccgtcaa acaacagccc cacgaggttg ccccgcgcat cgaaggtggg
     4201 gctgcccgaa ttgccgcccg tagtgtgcga ggtggagata aaacataccg gcatacgacc
     4261 gttgtcggca tattggccgt agtctttggc cgcgtacagg ctgcgcagtt tttccggcac
     4321 gtcaaactcg taatcgcccg gcttgtactt ttccatcacg ccgtctaagt aggtcatcgg
     4381 ctcatagcgc actgcatcgc ggggtcgata gcccatcact ttgccgtaag aaacgcgcag
     4441 ggtgctgttg gcgtcgggcg ccattttctt ttcagggaaa acatctatca gtgcctgcat
     4501 gtaagcgcgc tgcaggggcg tgatgcgctc ctgtatttcg gttgccggac gcatgatctt
     4561 gtcgttgcca gtgttgataa tttcgcgata cagcttcagg gccgggtcgt tttgcaattc
     4621 ctccaaccct ttgtcccagt tggtttccag cagcgccaag ccattattcg ggctgctcag
     4681 catggtagat gcatagatgg tctccgtcag tttggctgcc gagcgcccgt gtttattgta
     4741 caagtcaatg gcgtactgtg gttggtaggc agcgggtacc ttatcgaaat aaatgccaat
     4801 gagcagttca aacagttggc ggtccacctc cacgcggtag tctttgtaat agtttttcat
     4861 agccccgatg aattgggccg atcttttctg agcggcttcc gcaccctgcg tttccttaaa
     4921 tcgcaatgca ccatccacca tagaggccag acggaacaac tcaatctggc gggtgctctc
     4981 gttgaaatga tcgcggtgaa tgcccagcgg cgtcagttca gcgtagaggc ggtccaattc
     5041 gggcagaatc tggccgtatt tcgcattcag ggcggcattg gcggcccggc gcttgaactc
     5101 tgcctccata cggcgctttt gttccaagcc attgacgcgc ttgaggccct gcgtttcgcc
     5161 gatccatttt ttccaggcgt tggaaatgcc cgcctgtttg gaagtgtatt gcagtttcac
     5221 ctccgcatcg gcgcgcatag cggccccgat aatgccgagt atttggtcgc ggatgccggt
     5281 gcgaatggga ttgagtacat tcacctgcat ttcgacggca gaggcgggca ggtacaaatt
     5341 ggtacgcccc gggaacccgt aggtgagtga aaaatcgccc tctttgatgc cgcccagaga
     5401 gatgggcagg aagtgtttgg ggcgatacgg cacattgtcg gcagagtagg cagccgggcg
     5461 gttgtttttg tcggcataga tgcggaacac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnga tgtcgttgaa ggtttcggtc acaaaaagga agtagcggtt gccttcataa
     5641 aatgggcgga tgaaagcgct ttgccagctt tctttctgtg cctgtccgcg cacggtttcc
     5701 agatttttat caatggccga ctggcgctct ttttccggcg caccggccgt aacgcctgcc
     5761 agtactgcct gcgtaacttc ctcaatgcga acaatgaagg taacaaaaag tccctggctg
     5821 ggcagttctt cgctgaggct tttggcccaa aaaccgtcct ccagatagtt cttctccggc
     5881 gtagagagcg cctgtatgcg gccgtgaccg cagtggtggt tggtgagcag cagcccctga
     5941 gccgatacga cgccacccgt gcagcctccg ccgaagctga ctacggcatc tttgagactg
     6001 cctttgttga tgctgtacac atcttcggcg ctcattttca tgcccaattt tttcatttgc
     6061 ggctcattca tttctcgcag cagcagaggc agccacatgc cctcatctgc tcgcaggccg
     6121 gtgctgataa tggtcagcat agccactcag atgataatct ttctgatcat gttgtttttt
     6181 gttttccgta aaaagcggca agttagaaac gtaacagcaa tttttcagca acataagtct
     6241 ccggacgaaa acagccgaca cttttttcat ctcgctcctc acccactcac ccactcacca
     6301 ctcacccctc acccactcac ccctcaccta ctcacccctg t
//
LOCUS       DAORLF010000288         6311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6698_length_6311_cov_1.905371, whole genome shotgun sequence.
ACCESSION   DAORLF010000288 DAORLF010000000
VERSION     DAORLF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_6311_cov_1.905371"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK198_18995"
     CDS             <1..505
                     /locus_tag="PK198_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF40889.1"
                     /translation="NVLQLWESSLTLQRGEFLKVKGSVDTRLYFVESGCLRVFLMPEE
                     EELTIRFAYQNNLFVALDSFISEAPSDLYIQAIKKTKLRVMSKSAFTRLLNSEADAAH
                     WWRDILEVLVFQQFERETDLLISSPAERYRRVLARSPQLFQEVPHKYIASYLRMTPET
                     LSRLKKS"
     assembly_gap    588..597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <598..1119
                     /locus_tag="PK198_19000"
     CDS             <598..1119
                     /locus_tag="PK198_19000"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF40890.1"
                     /translation="ELTLMSTRHLAEARRWQKLSLEQLNQQPHPGAWSALECLEHLNL
                     YGDYYLPAMQQQMQKSRLSPQQHFRSGLLGNYFANSMLPKDKLDKMKTFQNMNPAGSQ
                     LDAGALERFIEQQELLLQILEQARNTDLGRIRIPITISRWIRLKLGDTLRFVIYHNER
                     HIQQALRALNLKK"
     gene            complement(1182..1556)
                     /locus_tag="PK198_19005"
     CDS             complement(1182..1556)
                     /locus_tag="PK198_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRF40891.1"
                     /translation="MSFEVSGKLIKKFDTENKTGTFQAREFVIEIEGQYPQVIKFQLV
                     QDRCSLLDAYNEGEVIKVHFDLRGREWNGKYLTNLNAWRLEKGDAAAPPAQATAAATP
                     PPPSSFPTVADEPPGADDDLPF"
     gene            complement(1753..2409)
                     /locus_tag="PK198_19010"
     CDS             complement(1753..2409)
                     /locus_tag="PK198_19010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF40892.1"
                     /translation="MRQGVAMHPPAILLETPQLLVVHKPAGMAVERQLHGYPSVEDWA
                     WQYLSASTKKPFIGIVHRLDRPVSGILLLAKKRAALKDLNLQFAERRTEKTYHAIVQQ
                     APYPAAGTLIHWLAKDTEGKRAVIAREGATGAARCELKYKILKDIPSGILLEIKPVQG
                     RFHQIRAQLAAAGTPILGDARYGAEASWQPDAIALHAHSLRFRDPADGQWRTVTAEWE
                     "
     gene            complement(2394..>2613)
                     /locus_tag="PK198_19015"
     CDS             complement(2394..>2613)
                     /locus_tag="PK198_19015"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF40893.1"
                     /translation="EGPICRFIAKKGPGLHHLAFAVDQVDAALQTVEERGVQLIDKTS
                     RRGAENLRIGFLHPKSTFGTLTELCGKE"
     assembly_gap    2614..2626
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2627..2806)
                     /locus_tag="PK198_19020"
     CDS             complement(<2627..2806)
                     /locus_tag="PK198_19020"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF40894.1"
                     /translation="MEISHIEHIGIAVENLQEAIAYYEEKLGLKCYSIEEVPDQKVRT
                     AFFKVGQTKVELLEST"
     gene            complement(2876..>3510)
                     /locus_tag="PK198_19025"
     CDS             complement(2876..>3510)
                     /locus_tag="PK198_19025"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF40895.1"
                     /translation="NFPVAVHAQGDRANDEVVDVFKKIHAKNAINHYRHRIEHCMLLP
                     KERLSDMKNMSITPSFHINHILYYGDFLDKEILGEERAARIFPLQSTLEAGLPYSLHA
                     DMPQFVPSPLALAHTSVHRQTESGAVYNENERVSVMQALKSITIHAAWQLHLEDKLGS
                     IEKGKYADLVILDQNPLKTTSEQMTSIRVLETIVAGNTVWKQQAAPGAAQ"
     assembly_gap    3511..3522
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3523..3831)
                     /locus_tag="PK198_19030"
     CDS             complement(<3523..3831)
                     /locus_tag="PK198_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40896.1"
                     /translation="MYQLLALAGKLPKRAPNPRLFIYLRKDNFDLLPEKARNTDDFYK
                     IIGIKMWYDGSPYSGSMFLKQPYIQSKFTIEGIHLSPNHTSESLLTEEELQGWIEKIQ
                     A"
     assembly_gap    3891..3932
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3933..4694)
                     /locus_tag="PK198_19035"
     CDS             complement(<3933..4694)
                     /locus_tag="PK198_19035"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF40897.1"
                     /translation="MKKHRTASSAGINKKTALILLIATVLAIVFYRVVLSDFSKDSDA
                     VYFGGDILTMSDEQPFVEAVYVKNGKIEAIGPKADVLKRITKNTKHIDLQGKTLMPGF
                     FDAHGHYDLAAVFYSMTDVSGVSYRRPQEVWARIENAVKNAPAGQWVYCYGFDPILTA
                     GVENPTLAYMDSIAPNNPLVIITKALHVFYVNSQAFSALGITNKTPDPSAASYYEKDA
                     AGKLTGAIVEQEALEPIRIQILADVMSNFVKNTRSI"
     gene            4801..>5215
                     /locus_tag="PK198_19040"
     CDS             4801..>5215
                     /locus_tag="PK198_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF40898.1"
                     /translation="MKIIIVLMAALGAVLLLSCFDSNKIASRPMENSTAQPSGKSFYD
                     FTVNSLSGEPVSLEQFRGKKIIILNVASKCGYTPQYADWEKFYAENKDEVVVLGFPCN
                     DFMGQEPGTANDIAEFCQKNYGVSFPMFEKVHVKGE"
     assembly_gap    5216..5258
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5406..>5621)
                     /locus_tag="PK198_19045"
     CDS             complement(5406..>5621)
                     /locus_tag="PK198_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRF40899.1"
                     /translation="PAVAAATNVERGALRLLPETDRASAGIYTDCDAQGNYHVWASVA
                     GTSALKRARLSSSTHFQLAEKVAAQFI"
     assembly_gap    5622..5637
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5638..6231)
                     /locus_tag="PK198_19050"
     CDS             complement(<5638..6231)
                     /locus_tag="PK198_19050"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40900.1"
                     /translation="MNQDIRILSRHTVLPASIAEALQNEAILCTLEPAASIGEICDAL
                     LRGTADTALLPLHQVSLTLPEGVVITALTERERPAECLLSLPDSVVASGMFGLPQNPV
                     IGLSGLRRSVQMKDLLPDYQEVELEGSFEEALHELRSGAVHALLWPEMDLPETGALQE
                     TGLNVLRFNPREFQPAPGQGVWAWLACRDDVAIRRLLK"
BASE COUNT         1404 a         1556 c         1695 g         1520 t
ORIGIN      
        1 aaacgtgctg caactctggg aaagcagcct gacactgcaa cggggcgagt ttctcaaagt
       61 gaaaggcagc gttgacaccc ggctgtattt tgtggaaagc ggctgtctgc gggtcttcct
      121 gatgcccgaa gaggaagaac tcaccattcg ttttgcatac caaaacaacc tgttcgtagc
      181 actcgattcg ttcatcagcg aggcgccttc cgacctgtat atacaggcca tcaaaaaaac
      241 gaagctccgg gtgatgagca aatccgcctt cacccgcctg ctgaacagcg aagccgatgc
      301 cgcacattgg tggcgcgata ttttggaagt gttggtcttt cagcagtttg aacgcgagac
      361 agatttgctc atcagttcgc cggctgaacg ctaccgccgg gtgctggccc gaagcccgca
      421 attgtttcag gaagtgccgc acaagtacat tgcctcgtac ctgcgcatga cgcccgaaac
      481 cctctcgcgg ctcaaaaagt cttgatttcg atcaagattg tatcgagtgc gccgaccgac
      541 ctttgtgaaa aatattccga ccatgaaaat gtctgctgcc accctgcnnn nnnnnnngag
      601 ctgacgctga tgagcacccg ccacttagcc gaagcgcgcc gctggcaaaa actgtcgctt
      661 gaacaactca accaacaacc tcatcccggc gcatggagtg cgctggaatg tctggaacac
      721 ttgaatttgt atggagatta ttacctgccg gccatgcagc agcaaatgca gaaaagcaga
      781 ttgtcaccac agcaacattt ccgcagcggc ttgctgggca actacttcgc caacagcatg
      841 ttgcccaaag acaagctcga caaaatgaag acttttcaga acatgaatcc cgccggcagc
      901 caattggacg ccggggcact ggagcgcttt atcgaacagc aagaactact gctgcaaata
      961 ctcgaacagg ccaggaacac cgacctcgga cgcatcagga tacccatcac catctcccgc
     1021 tggatacgct tgaagttggg ggatacactc cggttcgtca tttaccacaa cgagcggcat
     1081 atacagcagg cgctgcgcgc gctgaaccta aaaaaataag tgaaggggtg actacctgag
     1141 atagtcgcga agcaagtcac cccttcacta cccttttttc atcagaacgg cagatcgtcg
     1201 tcggcgccgg gaggctcatc ggctacggta gggaaactgc tgggcggcgg cggagtagcg
     1261 gcagccgtag cctgagcggg cggagcggca gcatcgcctt tttccaaacg ccaggcattg
     1321 aggttggtca ggtatttacc gttccattct ctgccgcgaa gatcgaagtg aactttgatc
     1381 acttcgcctt cattgtaggc gtcgaggagg ctgcaacggt cttgaaccaa ctggaattta
     1441 atgacctggg gatactgccc ttcaatttcg attacaaact cgcgggcctg aaaagtaccc
     1501 gttttgttct cggtatcgaa cttcttgatg agctttcctg aaacttcaaa agacatagcg
     1561 ttaaaggttt tgattttaga ggttcaaata tacaagcact ctttgttttg taataggcag
     1621 gggggatttt ttgttgaaaa aaagtaattg tttgacatat tcctcaagct gcaagttgca
     1681 ggctgcaagc ctcaagcctg ttcttcgtct taaaataagg tagtccgata catcaagtcc
     1741 acgacttcat agtcactccc actccgccgt taccgtgcgc cactgcccgt cggcggggtc
     1801 gcggaagcgg agcgaatggg cgtgcagggc gatggcatcg ggctgccagg atgcctcggc
     1861 gccgtagcgg gcatcgccga gaatgggcgt gccggcggcg gccaattgtg cgcgtatctg
     1921 atggaagcgg ccttgtacgg gcttgatttc cagcaaaatg cccgatggga tatccttcag
     1981 tattttatac ttcaactcgc agcgggcggc cccggtggcg ccttcgcgag cgatgacggc
     2041 gcgtttgccc tctgtgtctt tagccagcca gtgtatcaat gtgccggcgg cagggtaggg
     2101 ggcctgctga acaatggcat ggtaggtctt ttccgttcgg cgttcagcaa actggaggtt
     2161 gaggtctttg agggcggcgc gttttttggc cagcaggagt atgccgctca cggggcggtc
     2221 gaggcgatgc acgatgccga tgaagggctt ttttgtggag gctgaaaggt attgccaagc
     2281 ccagtcctct accgaggggt agccgtgcag ttggcgttct accgccatgc cggccggttt
     2341 gtggacgacg agcagttgcg gtgtttcgag gaggatggcg ggagggtgca tggctactcc
     2401 ttgccgcaca gttcggtgag cgtgccgaaa gtggacttgg gatgcaggaa tccgatgcgg
     2461 aggttttcgg cgccgcgccg cgatgttttg tctatgagtt gcacgccgcg ttcttctacg
     2521 gtttgcaggg cggcgtccac ttggtccaca gcgaaggcga ggtggtgcag gcctgggcct
     2581 tttttggcga tgaacctgca gatggggcct tccnnnnnnn nnnnnnggta gattcgagga
     2641 gttctacttt ggtttggcct actttgaaaa aggcagtccg tactttttgg tcgggcactt
     2701 cctcgatgga gtagcatttc agtcccagtt tttcctcgta gtaggcgatg gcttcctgca
     2761 ggttttcgac tgcgatgccg atgtgttcga tgtgagagat ttccataagg ttgttgatta
     2821 aagaaccagc ctcaaagtta gggagttggc ggcgaagtgg cgatgacttt tgcaactatt
     2881 gcgctgcgcc gggagcggcc tgttgtttcc agacggtatt gcctgccact atggtttcca
     2941 gcacccgaat ggaggtcatt tgctcgctgg ttgttttcag cgggttttga tccaaaatga
     3001 ccaaatctgc atatttccct ttttcaatac tgcccaattt atcctccaga tgcaactgcc
     3061 aggctgcatg aatggtgatg cttttgaggg cctgcatgac cgaaacccgt tcgttttcat
     3121 tatagactgc cccgctttcc gtttggcgat gaacggaggt gtgcgccagc gccaaaggac
     3181 tgggtacaaa ctgcggcatg tcggcatgga ggctgtaggg cagcccggct tccaacgttg
     3241 attgcaatgg gaaaatgcgg gcggctcgtt cttcgccgag gatttctttg tccaaaaaat
     3301 cgccgtagta caaaatgtga ttgatatgaa agctgggcgt tatgctcatg tttttcatgt
     3361 ccgacaggcg ttctttgggc agaagcatac agtgttcgat gcggtgccgg tagtggttga
     3421 tggcgttttt tgcatgaatt tttttgaaga catctaccac ttcatcgttc gctctgtcgc
     3481 cctgagcgtg aacggctacg ggaaaatttt nnnnnnnnnn nncgcctgaa ttttttctat
     3541 ccagccttgc aattcctcct cggtcagcag gctttcgctg gtgtggttgg ggcttaaatg
     3601 gatgccttcg atggtgaatt tagattgaat atacggctgc ttcaaaaaca tggaaccgct
     3661 gtatggagag ccgtcgtacc acattttgat gccgatgatt ttgtaaaaat cgtctgtatt
     3721 ccgggccttt tcaggcaaca ggtcaaagtt gtcctttcgg agatagatga acaaacgggg
     3781 attgggggcg cgtttcggca gttttccggc aagggctaaa agctggtaca aaggcttggg
     3841 tttttcggct gccaagtgtt cgtacaggct aaagatcgtt tttttattgg nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatgctgc gggtgttttt tacaaaattg
     3961 gacatcacat cggccaaaat ctgaatgcga atgggttcga gggcttcctg ctcgacgatg
     4021 gcgccggtga gttttccggc agcgtctttt tcgtaataac tggccgccga gggatcgggc
     4081 gttttgttgg taatacccaa tgccgaaaat gcctgagaat tgacataaaa aacgtgtaaa
     4141 gccttggtaa tgatgaccaa aggattgttg ggggcaatag agtccatgta ggccaaagtg
     4201 gggttttcca ctcctgcagt caggatgggg tcgaatccat agcaatacac ccattggccg
     4261 gcaggggcgt tttttacggc attttcaatc ctggcccaaa cctcctgcgg cctccgatag
     4321 ctgactccgc tgacgtcggt catggagtaa aaaacggctg ccagatcgta atgcccatgc
     4381 gcgtcgaaaa agcccggcat gagggttttt ccttgtaaat caatgtgttt tgtgttttta
     4441 gttattcgtt tcagtacatc ggctttgggg cctatggctt caattttgcc attttttaca
     4501 taaacggcct ccacaaaagg ctgctcgtcg ctcatggtta gaatatcgcc accgaaatac
     4561 acggcatcgc tgtctttgga gaaatcagac aagacaacgc gataaaatac gatggccaaa
     4621 acagtggcaa tcagtaaaat cagcgctgtt tttttgttaa tcccggcaga cgaagcggtg
     4681 cgatgctttt tcataataag ttggttttgc ttacaaaggt aaacgcgttc cttgttttgt
     4741 gtttgaacca tagcgcagat tccacgtttc aggggcatta cttttaaaac cgtattgcca
     4801 atgaaaatca tcatcgtcct catggcggcc ctcggcgccg tactgctttt gtcttgtttc
     4861 gattccaaca aaattgcatc gcgtcctatg gaaaacagca ctgcgcagcc ttccggcaaa
     4921 tctttttacg atttcaccgt caattctctc agcggcgaac ctgtttcgct ggagcaattc
     4981 cggggcaaaa aaatcatcat tctcaatgtg gcttccaagt gcggctatac tccgcaatac
     5041 gccgattggg aaaagtttta tgcagaaaat aaagatgaag tggtcgtgct gggcttcccc
     5101 tgcaatgact tcatggggca ggaaccgggt acggccaacg acatagccga gttttgccaa
     5161 aaaaactatg gcgtgagttt cccgatgttt gaaaaagtgc atgtaaaagg cgagcnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cggcgcaaaa cggatggaac
     5281 agccaggagc cgagttggaa tttttgcaag tacctcatca atgaaaaagg cgaactgacc
     5341 catttcttcg cgtccaaagt gaagccgagc agcgaggagt tcatcgcggc gatgggccag
     5401 taatatcaaa taaactgcgc cgctaccttt tcggccaatt gaaaatgcgt gctggacgac
     5461 agtctggccc gtttcaaggc tgatgtgccg gccaccgagg cccaaacgtg gtaattgcct
     5521 tgcgcgtcgc aatccgtata aatgcctgcc gatgcccggt ctgtttcggg caataaacgc
     5581 agggcgccgc gctccacatt ggtagcggcc gctacggcgg gnnnnnnnnn nnnnnnnttt
     5641 gagcaagcgc ctaatggcta catcatcccg gcaggccaac caagcccaaa cgccctgccc
     5701 cggcgcgggc tgaaattcgc ggggattaaa acgcagcaca ttcagccccg tctcttgcaa
     5761 tgccccggtt tcgggcaggt ccatttcggg ccaaagcagc gcatgaacag cgccggaacg
     5821 caactcgtgc agcgcttctt caaaactacc ctcaagttct acctcctgat aatcaggcaa
     5881 taagtctttc atttggacag aacgccgcaa gccggacagg ccaataacgg ggttttgcgg
     5941 caacccgaac atgcccgatg cgaccacact atcgggcaga ctcagcagac actccgcagg
     6001 gcgctcccgc tccgtcaagg ccgtgatgac cacgccttcg ggcaaggtga gggagacttg
     6061 gtgcaatggc agcagcgccg tatctgccgt gccgcgcaag agggcatcgc atatttcccc
     6121 gatggatgca gccggctcaa gggtacataa tatggcctca ttttgcaaag cctcggcgat
     6181 ggaggcgggc agcaccgtat ggcggctcag gattctaata tcttgattca tagtggtctg
     6241 tttccttaaa ggctcatctc ctccgctttc caatcctcaa tatttcgggc cgcgctgctg
     6301 aaattcacgc c
//
LOCUS       DAORLF010000289         6228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6803_length_6228_cov_2.096225, whole genome shotgun sequence.
ACCESSION   DAORLF010000289 DAORLF010000000
VERSION     DAORLF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6803_length_6228_cov_2.096225"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK198_19055"
     CDS             complement(<1..>214)
                     /locus_tag="PK198_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40901.1"
                     /translation="LRAGTVNVCADCPVKTIKEGIRQAQAGDVVLIGSGLYAEGNIVV
                     DKPLTLRGVNYPVIDGQLTEEPSPSPP"
     assembly_gap    215..319
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(377..808)
                     /locus_tag="PK198_19060"
     CDS             complement(377..808)
                     /locus_tag="PK198_19060"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRF40902.1"
                     /translation="MKKLFYLSIMILAAAACTPTAKPIEYGADACYYCKMTIVDRQHA
                     AEAVSAKGKVFMFDAIECMVPYLALEGEDNYPVLVVNDYLKPGEWADARSATYLISKG
                     LPSPMGAFLTGFADRAAAEPVQQEKGGELYNWESMKQAIAR"
     gene            complement(805..1380)
                     /locus_tag="PK198_19065"
     CDS             complement(805..1380)
                     /locus_tag="PK198_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40903.1"
                     /translation="MRQLPRWTMLVAALSLGALFVWPMWKIVLYAPQYPTGVEMHIWI
                     NKIGGNEPGVLQNINILNHYVGMKFIEPDSIPELKYFQYIIMAMMALGVWVALLNRRP
                     LFLGWAVLMTLLAIAGLYDFYLWEYDYGHNLSPDAPIKIPGASFQPPLIGYKQILNFD
                     AVSLPHIAGYFAGAGIALAYLAWWLHKKFSK"
     assembly_gap    1454..1591
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1592..3481)
                     /gene="nosZ"
                     /locus_tag="PK198_19070"
     CDS             complement(<1592..3481)
                     /gene="nosZ"
                     /locus_tag="PK198_19070"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRF40904.1"
                     /translation="MQNQFRIRWAIAAVIAMAILPAFFSGCGGPAVKTGALSGDEASR
                     VYVKPGEYDEFYAFISGGFSGQLSVYGIPSGRLLKVIPVFSLDAEKGYGFNEETKPLL
                     NTSHGFIPWDDSHHPELSQTDGETDGRWCFINGNNTPRIARIDLSTFETTEIIEIPNS
                     GGNHSSPFTTENTEYVVAGTRFGVPYPQKDVEIASYAENFKGMLSFISVDKESGRMDV
                     AFQVLLPAFNYDLAHSGKGKSHGWTFFTSYNTELKNTLLEVNASQNDKDFVAAINWKK
                     AEEYIKQGKFKEIPANYAHNVYDEASHVATTTMKKTVKVLIPEECPGLVYFLPTPKSP
                     HGVDVDPTGEYIVGSGKLSADLTVHSFTKVLAAIEKKSFETTIAGVPVLKYDEITAGV
                     VQKPGLGPLHTEFDGKGNAYTTFFISSEVVKWKVGTWEVVDRAPCYYSVGHLMIPGGD
                     SKKPDGKYLVAMNKITKDRYLPTGPELTQSAQLYDISGDKMKLLLDFPTIGEPHYAQG
                     IAATKIVPRSKKIYPLEENKHPYRAMGEKDAKVVREGNVVRVYMTMIRSHFAPDNIQG
                     IKVGDKVYFHVTNLEQDWDTPHGFSVLGAQNAELLIMPGQTETLLWEPKSVGVFPFYC
                     TDFCSA"
     gene            complement(3665..>3956)
                     /locus_tag="PK198_19075"
     CDS             complement(3665..>3956)
                     /locus_tag="PK198_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40905.1"
                     /translation="ARLQQRPTKNKWIVLISDGKPNDYDKYEGKYGIHDVKQALRELN
                     ARQIHSYALAIEAQAKYYLPQMFGQNHYQILTTPTELLQSLVKLYDKIRHQI"
     assembly_gap    3957..3999
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4000..>4313)
                     /locus_tag="PK198_19080"
     CDS             complement(<4000..>4313)
                     /locus_tag="PK198_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40906.1"
                     /translation="LHSRKTPSEKIYLSNRKREKDFSILLLLDISLSSDGYAAGSRVI
                     DVEKEVSILFGEILNEFHIDFSIDSFYSKTRNHSSYLILKDFDENWSVAKNKIGAVQP
                     CA"
     assembly_gap    4314..4399
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4400..5443)
                     /locus_tag="PK198_19085"
     CDS             complement(<4400..5443)
                     /locus_tag="PK198_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40907.1"
                     /translation="MGLELDELLYGWVVKRFKKKRRAAAESQAQRVELAPLMSRLTIV
                     ARAITGKAIDIFPAEREGGYKNNNFFLPPAFAELPDAPANTAFYFYRVLYMSVQQRLG
                     YNWQTQTEDLALSRQKALESSSLILKVLFEDYPAARHIHDTLRQHLESTAPANTPPDY
                     FWLYGKWMYNVPETDDNLRLQHVSGPDGERHGAPPDTTLKAKAVEEVKSLTIDKKQQE
                     DYVLLHNFEKVETAEEFNGTWRDFDGSDQLEEHQDALEELNMKFTVRTDDPTHSVYQA
                     DFVENVTVLESAEMESEGRFYTYDEWDYAGRCYKPDFCKVYPHRQLKSDAAYCVNTLN
                     KYGSTLMGLRKMLT"
     gene            complement(5466..>6228)
                     /locus_tag="PK198_19090"
     CDS             complement(5466..>6228)
                     /locus_tag="PK198_19090"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRF40908.1"
                     /translation="YYHRIHKETDVFEHAFRNKIPCLLKGPTGSGKSRFVEFMAHQLG
                     KKLITISCHEETSSTDLIGRFIIKGAETVWIDGPLTTAVREGAILYMDEVAEARPDVL
                     VAIHSLTDHRRELFIDKLGETVSAHEDFMLAASFNPGYQRGFKELKPSTRQRFIALSF
                     GYPEPKIETEILIKETGADPDVARKLVAIGNKIRNLTELGLAETVSTRLLVDAAKLIH
                     SGLPRRLSVHVAVVEPLTDAPTTIEALRDLADLMI"
BASE COUNT         1278 a         1545 c         1563 g         1470 t
ORIGIN      
        1 ggcggtgatg gtgaaggctc ttcggtcagt tggccgtcaa tgacggggta attcacaccg
       61 cgcaaagtca gcggtttatc caccacgatg ttgccctcgg catacaagcc cgatccgatc
      121 agcaccacat cgcctgcctg cgcctgccgg atgccctctt tgatggtctt gaccgggcag
      181 tcagcacaca cgttcaccgt gcctgcccga agccnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnt gcagcttacg gcttgaggct tacggcttgt agccgttcct
      361 accagccacc agcccctcac ctcgcaatag cctgcttcat gctttcccaa ttgtacaatt
      421 ccccgccctt ctcctgctgc accggctcgg cggccgccct gtcggcaaac cccgtcagaa
      481 atgcgcccat cggactgggc aatcccttgc tgatcagata ggtagcgctg cgggcatcgg
      541 cccactcgcc gggtttcaga tagtcgttca ccaccagcac cggataattg tcttccccct
      601 ccagcgccag atacggcacc atgcactcga tggcgtcaaa catgaacacc ttgcctttgg
      661 cgctcaccgc ctcggcagcg tgttggcggt ccacgatggt cattttgcag tagtagcagg
      721 catctgcgcc gtattcaatg ggcttggccg tgggcgtgca ggctgctgcc gcaaggatca
      781 ttatggagag ataaaagagc tttttcattt ggaaaacttt ttgtgcagcc accaggccaa
      841 ataagccaat gcgatgcccg ctccggcaaa gtagcccgct atatgcggca gcgaaacggc
      901 atcgaaattc agaatctgtt tgtacccgat cagcggcggc tgaaacgatg cgccgggtat
      961 tttgatgggc gcgtccggac tgagattgtg cccgtaatcg tattcccaca aataaaaatc
     1021 gtacagaccg gcaatggcta aaagcgtcat cagcaccgcc cagccgagga acaatggcct
     1081 ccggttgagc aaggcaaccc acacgcccag cgccatcatg gccatgatga tgtattggaa
     1141 gtatttcaat tcgggtatgg agtccggctc aatgaatttc atgcccacat agtggttgag
     1201 gatgttgatg ttttgcaaca cccccggctc attgccgcct attttgttga tccagatatg
     1261 catctccacc ccggtaggat attgaggcgc gtacagcacg attttccaca tcggccacac
     1321 aaaaagcgcc cccaacgaca acgctgccac cagcatcgtc catcgcggta attgtctcat
     1381 aattatttga ttttggagag ggggcaagcc ccgctgcaaa ccgcagtctt ttccaacatg
     1441 cggcttgcag cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccgaaca gaagtcggta cagtagaagg
     1621 ggaagacccc cacagactta ggctcccaaa gcagggtttc tgtttggccc ggcatgatga
     1681 gcaattctgc attctgagcg cccaaaacgg agaatccgtg cggggtatcc cagtcttgct
     1741 ccaagttggt cacatggaaa tacactttat cgcccacctt gataccctga atattatcgg
     1801 gggcaaagtg actgcgaatc atggtcatat acacgcgcac tacattgccc tcgcgcacca
     1861 ccttggcgtc tttttcaccc atagcgcgat acgggtgctt gttttcttcc agcggataaa
     1921 tcttcttgga acgcggtacg atcttggtcg cggcaatgcc ctgagcgtag tgcggctcgc
     1981 cgatggtcgg gaagtcgagc agcagcttca tcttgtctcc ggagatatcg tacaactgcg
     2041 cagattgcgt cagttccggc cctgtaggca ggtagcggtc tttggtgatc ttgttcatcg
     2101 ccaccaagta cttgccgtcg ggctttttcg agtcgccgcc ggggatcatc aggtggccca
     2161 cagagtagta gcacggagcg cggtccacta cttcccaggt gccgactttc catttcacca
     2221 cctcagagga gatgaagaaa gtggtgtatg cattgccttt gccgtcaaac tcggtgtgca
     2281 aggggcctaa gccgggtttc tgcactacgc cggctgtgat ctcgtcgtat ttcagcaccg
     2341 gcacgccggc gatggtggtt tcaaacgact ttttctcaat ggctgccagc actttggtga
     2401 aggagtgtac ggtgaggtcg gcagagagtt tgccgctgcc cacgatgtat tcgccggtgg
     2461 gatccacgtc cacgccgtgc ggcgatttcg gagtgggaag gaaatacacc aatcccgggc
     2521 attcttcagg aatgagcact ttcaccgttt ttttcatggt agtggtggct acatgtgatg
     2581 cttcgtcata tacgttgtgc gcgtagttgg cggggatttc tttgaacttg ccctgcttga
     2641 tgtattcttc ggcctttttc cagttgatgg cggccacaaa gtccttgtcg ttttgggagg
     2701 cgttcacttc caacagcgtg tttttcaact cggtattgta ggaggtgaag aaggtccagc
     2761 cgtgcgattt gcctttgccg gagtgcgcca ggtcgtagtt gaacgcaggc aacagcacct
     2821 gaaaagctac atccatgcgg ccgctttctt tgtcaacgct gatgaaggaa agcatgcctt
     2881 tgaagttctc ggcatagctg gcaatctcca cgtctttctg cggatacggc acgccgaaac
     2941 gggtaccggc caccacatat tctgtgtttt cggtagtaaa cggagaggag tggttgccac
     3001 ccgaattggg aatctcgatg atttcggtgg tttcaaacgt actcaggtca atgcgggcga
     3061 tgcggggcgt gttgttgccg ttgatgaagc accagcgccc gtcggtttcg ccgtcggttt
     3121 gcgacagctc cgggtggtga gagtcatccc aagggatgaa gccgtgggag gtgttgagca
     3181 gcggtttggt ctcttcgttg aaaccgtagc ctttttcggc gtccaatgaa aacacgggaa
     3241 tcaccttgag caaacgcccc gacggaatgc cgtacacgct gagctgaccg ctgaagccgc
     3301 ccgatatgaa ggcataaaac tcatcgtact ctcccggttt tacatataca cgggatgcct
     3361 cgtctccgct gagtgcgccg gttttgacag caggtccgcc gcacccggaa aagaaggccg
     3421 gcaatatcgc catcgctatg acggcagcta tcgcccatcg aatcctgaat tgattttgca
     3481 tgatagatat tgtttggtta aaccatgaag taataaaaga cctttaagtc ttaatttaaa
     3541 aacaccgcaa atctcttgaa gaaaaaccag ctacccaatg acttttgtca gtgtgccgga
     3601 ggataaaaag cagtctatcc aaactatcgg gcttgaacct gcttccgacg cgtcaaatgc
     3661 gcatttatat ttggtgtctg attttatcgt acaacttgac caatgactgc aacagctctg
     3721 tgggcgtggt gagaatctgg taatgatttt gcccaaacat ttggggcaaa taatacttgg
     3781 cttgcgcttc aatagccaat gcgtaggaat gtatctgcct ggcattcaac tccctcagcg
     3841 cctgcttcac gtcatggatg ccgtacttgc cttcatattt gtcgtagtcg ttgggcttgc
     3901 cgtctgaaat cagcacgatc catttgttct tggtgggtcg ttgttgcaat cgggcgnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcacaaggct gcactgcgcc
     4021 gatcttgttt ttggcaaccg accagttttc atcaaagtct ttgagaatca gatacgaaga
     4081 gtgatttctg gttttggaat aaaagctgtc aatggagaag tcaatgtgaa attcattcag
     4141 tatttctcca aacaaaatgg acacttcctt ttccacgtca atcacacggc ttccggctgc
     4201 atagccgtcg ctcgacaggc tgatatcgag cagcagtaaa atggagaaat ccttctccct
     4261 tttccggttg gagaggtaaa ttttttctga gggcgttttg cgggaatgca ggtnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnng gtcagcatct ttcgcaggcc catgagcgtg gagccgtatt
     4441 tgttcagcgt gttgacacag taagcagcgt cggatttgag ctgcctgtgg ggatatactt
     4501 tgcaaaaatc cggtttgtag caacgcccgg cgtagtccca ttcatcgtag gtgtaaaaac
     4561 gcccctcgct ttccatctcc gcgctttcca gaacggtgac attttccaca aaatcagcct
     4621 gataaacgga gtgtgtgggg tcgtcggtac gaacggtaaa tttcatattg agttcttcca
     4681 gcgcatcctg atgttcttcc aactggtcgg agccgtcaaa atcgcgccaa gtaccgttga
     4741 actcctcggc ggtttctact ttttcaaaat tgtgcagcag cacatagtct tcctgctgtt
     4801 ttttgtcaat cgtcaatgat tttacctctt ctacggcctt ggctttcagg gtggtatcgg
     4861 gcggtgcgcc atgccgttcg ccatccggcc ctgagacgtg ttgcaaccgc aggttatcat
     4921 ctgtttccgg cacgttgtac atccatttgc cgtagagcca gaaatagtcg ggcggggtat
     4981 tggccggggc ggtgctttcg agatgctgcc ggagtgtgtc gtgaatgtgc cgggccgctg
     5041 gataatcttc gaataatacc ttgagaatga gcgatgaaga ttccagtgct ttttggcgcg
     5101 aaagtgcgag gtcttccgtc tgggtctgcc agttgtagcc caaccgctgt tgtacgctca
     5161 tgtacagcac tctgtaaaaa taaaatgcgg tattggccgg ggcatcgggc agttcggcga
     5221 aagccggcgg aagaaaaaag ttgttgtttt tataaccgcc ttcccgctcg gcaggaaaaa
     5281 tatctatggc cttgccggta atggcccgcg caacgatggt caatcgggac atcaacggcg
     5341 ccagttctac tcgttgagcc tgactttcgg ccgccgccct gcgctttttt ttgaaacgct
     5401 tcaccaccca tccgtagagc agttcgtcca attcaagtcc catgatgctt tcttttaaaa
     5461 aaaaatcaaa tcatcagatc cgccagatcg cgcaatgcct cgatggtagt gggggcatcg
     5521 gtgagaggct ccaccacggc aacatgcacg gagaggcggc ggggcagccc gctgtgaatg
     5581 agcttggcgg catccaccag cagacgtgtg gaaacggtct cggcaagccc caactcggtg
     5641 aggttgcgga ttttgtttcc tatggccacc aattttctgg ccacatccgg gtcggcgccg
     5701 gtttctttga tgagaatttc agtttcaatt ttcggttctg gatagccaaa agaaagagca
     5761 atgaaccgct gccgggtaga gggtttcaac tctttgaacc cccgctgata ccccggattg
     5821 aaagaggctg ccagcatgaa gtcttcgtgc gcactgaccg tttcgcccaa tttatcaatg
     5881 aacaactcgc ggcggtggtc ggtcagcgag tgtatggcca ccaacacgtc gggccgggcc
     5941 tccgccactt catccatata caagatggcg ccctcgcgaa cggcggtggt cagcgggccg
     6001 tcaatccata cggtttcggc gcctttgatg atgaaccggc caatcaggtc ggtagaggat
     6061 gtctcttcgt ggcaggaaat ggtgatgagc tttttgccca attgatgcgc cataaactcc
     6121 acaaaacgcg atttaccgct accggtaggc cctttgagca ggcagggtat tttgttgcgg
     6181 aatgcgtgtt caaaaacatc cgtttccttg tgtatgcgat ggtaataa
//
LOCUS       DAORLF010000290         6175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6886_length_6175_cov_1.743301, whole genome shotgun sequence.
ACCESSION   DAORLF010000290 DAORLF010000000
VERSION     DAORLF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_6175_cov_1.743301"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>241)
                     /locus_tag="PK198_19095"
     CDS             complement(<1..>241)
                     /locus_tag="PK198_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40909.1"
                     /translation="SVFPNPMGQSTTIAFKSLSTGRVRLSVFDAMGNELKVLLDQRLS
                     SGEHTLNWDASGLPAGVYFCRLVLDGRKQVRRVVKG"
     assembly_gap    242..256
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<257..>617)
                     /locus_tag="PK198_19100"
     CDS             complement(<257..>617)
                     /locus_tag="PK198_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF40910.1"
                     /translation="LRLLGIEQRVISITFSEFGRRIRSNAAVGTDHGTAAPLLMFGSC
                     VQPQVLGDNPEISANVSVDEGVPMQHDFRDVYGSVLMDWFDVPEEDVRTFLYDGFTRL
                     PIVAPCAVNAVEDTLDVP"
     assembly_gap    618..627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<628..1570)
                     /locus_tag="PK198_19105"
     CDS             complement(<628..1570)
                     /locus_tag="PK198_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40911.1"
                     /translation="MMQRRRFLKTTGAAVSIPLLLNGIRLSAMPRTSIFNAVDDANDR
                     VLVLIQLSGGNDGLNMVLPTDQYDGLAAVRSNILIPQNAILPLTDATGINPSMTSVKN
                     MYDNGRLAVIQAVGYPDQNRSHFRSTDIWTSGSPANQVWRTGWMGRYFDSQYPDYPEG
                     YPNTEHPHPFAITMGNLVSETCQGIATNFSLTLEDPFALGQLPEGQIGPVPNTPYGRE
                     LDFLRTAIAQTNQYATAITEVATAGNTLANYPVNRLAEQLQHVARLISGGLQTKIYVV
                     NLGGFDTHANQVNPDNPTQGTHVELLRTLSDAMAAFQE"
     gene            complement(1607..3268)
                     /locus_tag="PK198_19110"
     CDS             complement(1607..3268)
                     /locus_tag="PK198_19110"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF40912.1"
                     /translation="MDRRATLATILGQSKVKQSKHRAAPPVLTSLSPYTGAWGREQAS
                     HLLRRATFGPTKALIDAAVQNGLEVTFAQLFAEQPMLDPPVNHNNTTDPNVPVGQTWI
                     NAPYSATVNLVGYRNQSLRAWTFGLMVNEGLSIREKMTLFWHNHFSVNEINDPKFLYR
                     YINLLRGAATGNFRQLVKDITVDPTMLRFLNGNQNTRTAPNENYARELLELFTIGKGP
                     QAGPGDYTNYTETDVIQIARVLTGWRDRGFNTNNADVQVESFFTLTRHDLGVKQLSHR
                     FDNVNIPNMAENEYAHLVDIIFQKDEVARFICRKLYRWFVYYDISAATEAEVIEPMAQ
                     ILIANNYNIRPALEALLRSEHFFDILSIGPMIKSPMDFVVGAFRQFAIPYSPLIARRY
                     TAWFSLIRLTIPMQMEYFNPPSVAGWNAYYQEPGFYRIWINAATLGPRSRFTDTLATT
                     GFVYNTVRHVVNVLELVQTVSDPFDPVVLIREWSEWLYPQPLAENQLTYLKEVLLPGL
                     PDYEWTLEYNDYLNSPNDVNLRQAVETKLRNLLKAMLSLPEYYLS"
     gene            complement(3408..4472)
                     /gene="queA"
                     /locus_tag="PK198_19115"
     CDS             complement(3408..4472)
                     /gene="queA"
                     /locus_tag="PK198_19115"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRF40913.1"
                     /translation="MRTKLSQFSFELPKKLIAQYPAVNRDESRMMVVHRDSGKIEHRI
                     FKDIMEYFDDGDVMLFNNTRVFPARMYGQKEKTGAKIEVFLLRELNHDARLWDVLVDP
                     ARKIRVGNKLYFSDENDNEVLVAEVVDNTTSRGRTIRFLYDGTEEEFQRDLYTLGNTP
                     LPKYITRPAEESDKDRYQTVFAAETGAVAAPTAGLHFSKELMKRLEIKGLEFAELTLH
                     VGLGTFRPIEVEDLSKHKMDAEYFRIPQKAVDVVNKAKENNNKICAIGTTSMRAVETS
                     VSAEGFLKPAEGWTNKFIFPPYDFSIANSMVTNFHPSKSSLLIMVCAFGGFDLVMEAY
                     QEAMKEEYRFFSYGDSMLII"
     gene            complement(4469..>4680)
                     /locus_tag="PK198_19120"
     CDS             complement(4469..>4680)
                     /locus_tag="PK198_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40914.1"
                     /translation="AFTTGAVLISAGRASKASPKSGFRLALHKILDKKQLFICQSLEN
                     KFFKNGCNRLVCAYLCGQFLKKNHT"
     assembly_gap    4681..4746
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4764..>5225
                     /locus_tag="PK198_19125"
     CDS             4764..>5225
                     /locus_tag="PK198_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF40915.1"
                     /translation="MSQAFQQLRANKLRSFLSLLGISIGIFCIIGVTAAVDSLEDNVR
                     GSFEKLGDDVVYIQKFMWGDPGPNWQRYLRRPDVSFKDFEAVEEKAQSAQVVSYGTFI
                     GLKTFKFRSANVRDVEFQAVSYGFDELVKLDFQNGRYFSPTEYHYGSNKIII"
     assembly_gap    5226..5262
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5263..>5632
                     /locus_tag="PK198_19130"
     CDS             <5263..>5632
                     /locus_tag="PK198_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF40916.1"
                     /translation="DPIGRTIQMGGRKFDVIGVLEPAGTSLIDIFSFDNAAIISYELG
                     RKVANLRPDNPFGNASISVKAKEGVSIEALKDELTGIMRAQRRLKPREDDDFSLNTLS
                     IVSEALDGFFQVLSLLGIVIG"
     assembly_gap    5633..5649
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5650..5994
                     /locus_tag="PK198_19135"
     CDS             <5650..5994
                     /locus_tag="PK198_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF40917.1"
                     /translation="IFVGMFSVANIMFVSVKERTGIIGIKKALGAKRFVILLEFLIES
                     IVLSIFGGLVGLLLVQAVVKILSGIIDFNLFLSASNILTGIITSVVVGILAGLLPAWQ
                     AAKMDPVEAMRK"
BASE COUNT         1447 a         1527 c         1571 g         1485 t
ORIGIN      
        1 ccccttcacc acccggcgca cctgcttcct tccatccaac accaaacggc agaaatacac
       61 gcccgccggc agcccggatg catcccagtt gagggtgtgt tcaccgctgc tcaggcgttg
      121 gtccaacagt accttcaact cgttgcccat cgcatcgaat acgctgaggc gaacgcgacc
      181 tgtactcaga ctcttgaagg caatggtggt gctttgtccc atcggattgg ggaaaacaga
      241 cnnnnnnnnn nnnnnnccgg aacatccaat gtatcctcca ctgcattcac cgcacagggc
      301 gctacgatgg gcaaacgcgt gaaaccgtcg taaagaaaag ttctcacatc ctcttccggc
      361 acatcaaacc aatccatgag caccgaacca tatacgtcgc ggaaatcatg ctgcatcgga
      421 acgccctcat ccacagacac attcgcagaa atttcagggt tgtcgcccag tacctgcggt
      481 tgtacgcagg aaccaaacat cagcagtgga gccgccgtgc cgtggtcggt accaacggct
      541 gcgttggagc gaatgcgccg cccgaattct gaaaaagtga tgctgataac gcgctgttcg
      601 atgcccagca gccggagnnn nnnnnnnctt cctgaaaagc ggccatcgca tccgataaag
      661 tgcgaagcag ttccacatgc gtaccctgcg taggattgtc gggattgacc tgattggcgt
      721 gcgtatcgaa accgccgaga ttgacgacgt aaatctttgt ttgcaaaccg ccggaaatga
      781 gccgcgccac atgttggagt tgttcggcca atcggttcac cggataattg gccagcgtgt
      841 ttccggcagt ggctacctcg gtaatggccg tggcgtactg attggtctgg gcaatggcgg
      901 tgcgcaggaa gtccaactcg cggccataag gcgtattggg tacagggccg atctgcccct
      961 ccggcaattg ccccaacgca aaggggtctt ccaaggtcag actaaaattg gtggcaattc
     1021 cctggcaggt ttccgacacc aaattgccca tcgtgatggc aaaagggtgc gggtgttccg
     1081 tattggggta cccctccggg taatcgggat attggctgtc gaaatagcgc cccatccagc
     1141 cggttctcca cacctgattg gccggcgagc cggaggtcca gatgtcggta gaacggaagt
     1201 gcgaacggtt ttgatccggg taccccaccg cctgaatgac ggccaaccgc ccattgtcgt
     1261 acatattttt cacgctggtc attgacgggt tgataccggt ggcatcggtc aacggcagaa
     1321 tggcattttg cggtataaga atattgctcc gcacggctgc cagcccatcg tattggtctg
     1381 tgggcaacac catattgagt ccgtcgttgc ccccgctgag ttgaataagc accaaaacgc
     1441 ggtcattggc atcgtcaaca gcattgaata tggaggtgcg cggcatggcg ctcaggcgaa
     1501 ttccgttgag cagcagcgga atagaaacag cggcgcccgt agtttttaaa aaacgtctcc
     1561 tttgcatcat cttaaatggt tttgaagtgt ggcgtaatgg tgctttttaa ctcagataat
     1621 actcaggaag actgagcatg gctttcaaca ggttgcgcaa tttggtctca accgcttgcc
     1681 gcagattcac gtcattcgga ctattgaggt agtcattgta ttccaatgtc cactcataat
     1741 ccggcaaacc gggaagcaaa acctctttga gataagtcaa ctggttctct gccaaaggtt
     1801 gcggatacag ccattccgac cattcccgaa tcaagaccac cggatcgaaa ggatcgctga
     1861 cggtttgcac caactccagc acattgacca catggcgaac ggtgttgtaa acaaaacctg
     1921 tggtggccaa tgtatctgta aaccgcgagc gcggccctaa agtagcggca ttgatccaga
     1981 tgcgataaaa tccaggctcc tgataatacg cattccagcc ggccacgctg ggcgggttga
     2041 aatattccat ttgcatggga atggtcagtc ggatgaggct gaaccaagcc gtgtaccttc
     2101 tggcaatcag gggcgaatat ggaatggcaa actgcctgaa agcgcccacc acaaaatcca
     2161 tcggactttt aatcataggg ccgatgctga gaatatcgaa aaagtgttcg ctgcgcaaca
     2221 gggcttccag tgccgggcgg atgttgtaat tatttgcgat gagtatttgc gccatcggct
     2281 ctatgacctc ggcctcggta gccgcactga tgtcataata cacaaaccag cggtacagct
     2341 tgcggcagat aaaccgggcc acctcgtctt tttggaaaat gatgtccacc aaatgcgcgt
     2401 actcattttc ggccatattg gggatgttca cgttatcaaa acgatgcgat aactgcttga
     2461 cacccagatc gtggcgagtg agtgtaaaga agctctccac ttgcacgtcg gcgttgttcg
     2521 tgttgaatcc gcggtcgcgc cagccggtca gtactcgggc tatctggatc acatccgttt
     2581 ctgtataatt ggtataatcg ccggggcctg cctgcgggcc tttgccgatg gtaaagagtt
     2641 cgagcagctc gcgggcgtag ttttcattcg gcgccgtacg ggtattctgg ttgccgttga
     2701 gaaaccgcag catggtgggg tcaacggtaa tgtctttgac caattgccgg aagttgcctg
     2761 tcgccgcgcc tctcaacaaa ttgatataac ggtacagaaa cttgggatcg ttgatttcat
     2821 tgacagagaa gtgattgtgc caaaacaagg tcattttttc ccgtatggag agaccttcat
     2881 tgaccatgag gccgaaagtc caggcgcgca ggctttgatt gcgatacccc accaggttca
     2941 cggtagccga atagggcgcg ttgatccagg tttggcctac gggcacattg gggtcggtgg
     3001 tattgttgtg attaaccggc ggatccagca tcggctgctc ggcgaacaat tgcgcaaatg
     3061 tcacctccaa tccgttctga acggcggcgt ctatcagggc tttggttggc ccaaatgtgg
     3121 ctcttcgcaa cagatgcgag gcttgttcgc gcccccatgc tccggtgtaa ggcgacaagg
     3181 aggtgagcac cggcggcgcg gcgcgatgtt tggattgttt gactttggat tgccccagaa
     3241 ttgtggcaag agtagctctt ctgtccatcc cgtcaggttt ttggtggaga taaaagattg
     3301 catcgttctg catcagcttc actgatgatg agaaaggtgc aaatatgacg ggaaaaagtt
     3361 gcctgagttt aaaagaacgc aaaaataatt ttgcctccct ttcaatctca aataatcaac
     3421 atggaatcgc cgtagctgaa gaagcgatac tcctctttca tggcctcctg ataggcttcc
     3481 atcaccagat cgaaaccgcc gaaagcgcac accatgatga gcaggctgga tttggacgga
     3541 tggaagttgg tcaccatcga gttggcgatg ctgaaatcat acggagggaa gatgaactta
     3601 ttggtccagc cttcggcagg tttgagaaaa ccctcggcag aaaccgaagt ctccacggcc
     3661 cgcatggacg tagtgccgat ggcgcaaatt ttgttgttgt tttctttggc cttattgacc
     3721 acatccacag ctttttgtgg gatgcggaag tactcagcgt ccattttgtg tttagacagg
     3781 tcttccactt ctatgggacg gaaggtgccc aaaccaacgt gcagcgtcag ttcagcaaac
     3841 tccaaacctt tgatttccaa tcgcttcatc aattccttac taaaatgaag ccctgccgta
     3901 ggagcagcca cagcgccggt ttcagcggca aagacggtct gatagcggtc tttgtcggat
     3961 tcctctgccg gacgagtgat gtatttgggc agcggagtat tgcccagcgt gtagaggtcg
     4021 cgttggaatt cctcctccgt gccatcgtac aaaaagcgga tggtacggcc acgcgaggtc
     4081 gtattgtcaa cgacttcggc caccaacact tcattatcgt tttcgtcgct gaaatagagc
     4141 ttgttgccca cgcgaatttt tctggccggg tccaccagca catcccacaa acgagcgtcg
     4201 tggttgagtt cacgcaatag aaaaacttct atttttgctc cggttttttc cttttggccg
     4261 tacattctgg ccggaaacac ccgcgtgttg ttgaacaaca tcacgtctcc atcgtcaaag
     4321 tattccatga tatctttgaa gatacggtgt tctattttgc cgctgtcgcg atgtactacc
     4381 atcatgcggc tctcatcgcg gttaacagca ggatactggg cgatcagttt tttgggaagc
     4441 tcgaagctga attgagaaag cttggtcctc atgtatgatt ttttttcaaa aattggccgc
     4501 aaagataagc acaaacaagc cgattgcaac cattcttaaa aaatttattt tcaaggcttt
     4561 ggcagatgaa aagctgtttt ttatctaaga ttttgtgcag cgcaagccgg aaaccggact
     4621 ttggcgatgc ttttgaagct ctgccggcac ttataagtac agcgcctgtt gtaaaggcaa
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnaggt ttttttagaa agtatttcac aggcatttca acaattgaga gccaataagt
     4801 tgcgcagttt tttatcgctg ctcggcatct ccataggcat tttttgcatc atcggcgtta
     4861 cggctgccgt agattcctta gaggataatg tgcgaggcag ttttgaaaag ctcggcgatg
     4921 acgtggtgta tatccagaag tttatgtggg gcgaccccgg ccccaactgg cagcgctacc
     4981 tccgccgccc ggatgtgagc tttaaagatt ttgaagccgt agaagaaaaa gctcagtctg
     5041 ctcaggtcgt atcttatggc accttcatcg ggctgaagac tttcaagttc agatcggcca
     5101 acgtcagaga tgtggagttt caggccgtca gctatggttt tgacgaattg gtcaagctcg
     5161 attttcaaaa tggccgctac ttctccccta ccgaatatca ctatggctcc aacaaaatca
     5221 tcatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatcctat cgggcgcacc
     5281 atacaaatgg gcggcaggaa attcgacgtc atcggcgtat tggagcctgc cggtaccagc
     5341 ctgatagata tcttcagctt tgacaatgca gccattattt cttatgaact ggggcgcaaa
     5401 gtggccaatc tccgcccgga caaccccttc ggcaacgcat ccatcagcgt aaaagccaaa
     5461 gagggcgtga gcatcgaagc gctgaaagac gaactcacgg gcatcatgcg cgcgcaacgg
     5521 cggctgaaac ctcgcgagga tgacgatttt tcgctcaata cgctctccat tgtcagcgag
     5581 gcgcttgacg gattttttca ggtgctcagt ctgctgggca ttgtcatcgg cgnnnnnnnn
     5641 nnnnnnnnna tttttgtagg catgttcagc gtagccaata tcatgtttgt atccgtcaaa
     5701 gaaagaacgg gcatcatcgg catcaaaaaa gcgctgggcg ccaaacgttt cgtcattttg
     5761 ctggagtttc tgatcgagtc catcgtcctc agtatatttg ggggcttggt cggcttgttg
     5821 ttggtacagg cagtggtcaa aatcctctcc ggcatcattg acttcaactt attcctttcg
     5881 gcttccaata tccttaccgg catcattact tctgttgtgg taggcattct ggccggtctg
     5941 ctccccgcct ggcaggctgc aaaaatggac ccggtggagg ccatgcggaa gtaaatggta
     6001 ccgaggcttt taccgtatgc cactctccgg acagcggtaa tcatacaaat cataaagatc
     6061 agcgtcggaa aaaaaacgtt tttgacgcag aaagacgcag atttttatga tcgtcatgct
     6121 cctctccgag cagcgaaaat catacaaatc ataaagatca gcgtcggaaa acagg
//
LOCUS       DAORLF010000291         6142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6927_length_6142_cov_1.985050, whole genome shotgun sequence.
ACCESSION   DAORLF010000291 DAORLF010000000
VERSION     DAORLF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6927_length_6142_cov_1.985050"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2673
                     /locus_tag="PK198_19140"
     CDS             <1..>2673
                     /locus_tag="PK198_19140"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF40918.1"
                     /translation="FFISGPGINGPYQNNAINIARVPGTNLPVTINNVNPGVVGAAGE
                     LINCTPPRGSLAYGTFYNDNDGSNVFPIYDGITDVFVAEAVVQPCQVYTIKLVICDVS
                     DQVYDSGVFLEAKSFGTGSLNVDVATVSLDGSVAEGCSSANITFSLPNRVESDFPIDF
                     QLIGTAINGVDYEQIPTNLFIPAGDSSVSIEIRAIEDGITEGNELLMIDVQRDLCNRD
                     TVFIVVRDNPLTKPDLGQDPMICPDTPINLDGTIPVVLPPLPTFTNANNLTINPINTP
                     IFSNIQVTGVIPNVLSPNILRSICIDSLSHPWIDDIDVYLITPGGQFIELTTDNGGNG
                     GNGLGPDFYLHTCFTPNAATRINFPGPLAPPSAVPFTGDWLPEGEWSDIWGGPVNGQW
                     RLQLIDDTNSAVGTLHSWTLTFNRTYDVEYAWSPAAGLSCVDCPNPTAMPDQSVEYIL
                     RASDSYGCSVSDTIFFDVQSQLPAPELICGTATNNSVSVAWQDIPGAAGYEININNTG
                     WTTPSDALSHLIDGLSEGQTFEVMLRALGMCPGPADTLQCQALTCAPPDLSATVSDAS
                     CAGLSNGGVTLSTASGTGTLTFELNNQTNNTGTFSGLAPGLYVASMTDGANCPATVQF
                     TISAPQAMALSLSLADSVACFGQSNGSASVAVAGGTSNYTFAWDNGEATATATQLDAG
                     THTVTVTDMAGCVAESDIMIPQPAALSLSVNATAVVCPGAGNGTATVVPAGGTASYQF
                     VWDALAGNQNGATAQGLSGGNYEVIVTDANGCTAQISTTVAEPAAIQMTTQTTDAQCF
                     GTATGTASATATGGNGAFSFVWSNMQNTAAATGLSAGNYTVIATDAAGCRDTAAVQVN
                     QPTALQVQWQPADPACFGDADGSASATISGGTGI"
     assembly_gap    2674..2698
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2699..4957
                     /locus_tag="PK198_19145"
     CDS             <2699..4957
                     /locus_tag="PK198_19145"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF40919.1"
                     /translation="QAPNNHPVRQNLSAGSYVVTVSDANNCTETQTITLVNPPLLSAA
                     MTTSPADCNAAATGSAQATATGGTGAYTYLWSNGQNGPDAANLQAGPISVVVTDANGC
                     TTLATGSITEPTAMTLSIAPADARCFGASDGTATAIAGGGAGNYTYLWSNGQTGPAAS
                     GLGAGTYNLVVRDGNNCMISQSFNISQPTALNATTQSVTASCSPQPDGEASVSATGGT
                     QPYTYRWSDPAQQTDAAARQLPPGAYTVTVTDANGCSFTQNAAVAGSPAVQLQLQATP
                     VGCHGGNNGGISATATGGTGAFTYQWSAAGLGNVSNPAQLSAGLYSLTVTDERQCTGI
                     SQADVTQPAPLTLSAATQHVACAGNATGGIDLTVSGGTMPYRFAWSTGDNVEDLSQLS
                     SGVFSVTVTDANGCVANLETTVIQTTRITASFSTVRADCYGAATGAASVFVNGGSAPY
                     LYEWSNGGSGAAIQNVPAGLYQLAVTDGLGCRTEFEVAIEQPEQPLTASPNLMPPTCH
                     DEDNGRIELTTTGGTPAYEYSMNGGPFVGNRIFVALHAGVYTIAIKDAKGCLFNTGPL
                     QLADPAPLSVDLGPDVTLQYGDSLRIQPRILGAVEPLMLEWKPRDTLFFGCLSCPEPV
                     IRPLFQRAITLVVTDANGCTAEDLFTVFVVKDPRAFVPTGFTPNGDGMNDRLLVHGRQ
                     GTRVLSFRIFDRWGQLLYEGGDFEVNDSAFGWDGTFRGKPVTADTYIWSLTVELQDGD
                     KEILSGETNVIR"
     assembly_gap    5159..5173
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5174..5906
                     /locus_tag="PK198_19150"
     CDS             <5174..5906
                     /locus_tag="PK198_19150"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40920.1"
                     /translation="DVRITARFQRQTALHILKYLLTDTGLGWRVVGNQVVIYKLPPVP
                     ERKYTISGFLYDASSGEPLIGAWLMDQNSKKPAVTNVYGFFSLTLPAGEVNLLCSYVG
                     FEQKDTAFTLKKDVALVFHLRSAITLTEVVVKARSGASSAPQPQTQLLGTEIVEDLPA
                     LGGEADLVRGLHLLPGVQTGTDGVEGLHVRGGSQGHNLVLIDGVPVYNYGHAAGIFSV
                     FNTEAVRSVRFLKGSFPARYGGWLR"
     gene            5912..>6142
                     /locus_tag="PK198_19155"
     CDS             5912..>6142
                     /locus_tag="PK198_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40921.1"
                     /translation="MRTKEGNTRHFQGRAEMGMLSSRLTLEGPIVRDKSAFFISGRTS
                     LINWYLRPLSRNFKTEQGEQGQTLYNFDDINAK"
BASE COUNT         1287 a         1804 c         1708 g         1303 t
ORIGIN      
        1 gattcttcat cagcgggccg ggcatcaacg ggccttacca aaacaacgcc atcaacatag
       61 cgcgcgtgcc gggtaccaac ctgccggtaa ccatcaacaa cgtaaacccc ggcgtggtag
      121 gagccgcagg agaattgatc aattgcaccc cacccagggg gtcgctggct tatgggactt
      181 tttacaacga caacgacggc tccaacgtat ttcccattta cgacggtatc accgatgtat
      241 ttgtggcaga ggcagttgtg cagccttgtc aggtttatac catcaagttg gtgatttgcg
      301 atgtatcgga ccaagtgtac gattccggtg tgtttttgga ggctaagagc ttcggcactg
      361 ggtcattgaa tgtggacgtg gctacggtca gcttagacgg ctcggtcgct gagggctgct
      421 cctccgccaa tattactttc tctttgccca accgggtgga atctgatttt cccattgatt
      481 ttcagttgat aggaactgcc atcaacggcg tggactacga gcagattcct accaacctgt
      541 ttattcctgc cggcgacagc tcggtttcta ttgaaattcg agccattgag gacggaataa
      601 cggagggcaa cgaactcctg atgattgacg tgcagcgcga cttgtgcaac cgggataccg
      661 tttttatcgt tgtgcgagac aatcctctga ccaaaccgga tttgggccag gacccgatga
      721 tctgccctga tacgccgatc aatttggacg gaaccattcc ggtggttttg ccgcctttgc
      781 ccacattcac caatgccaac aacctgacca tcaaccccat caatacgccc atcttctcca
      841 atattcaggt cacgggcgtc attcccaatg tgctcagccc caacattctg cgctccatct
      901 gtatagacag cctctctcat ccctggattg acgacattga cgtttatctc atcacgccgg
      961 gcgggcagtt tatagaattg acaaccgaca acggcggcaa cggcggcaat ggactgggac
     1021 cggattttta tttgcacacc tgtttcaccc ccaatgcagc cacccgtatc aactttcccg
     1081 gcccgctggc cccgccctct gcggtgcctt tcaccggcga ctggctgcct gaaggcgagt
     1141 ggtccgacat ctggggcggg ccggtcaatg gccagtggag actgcaactc atagacgata
     1201 ccaacagcgc cgtaggaact ttgcacagtt ggacgcttac tttcaaccgc acttacgatg
     1261 ttgagtatgc gtggtcgccg gctgccggcc tgagttgcgt tgattgcccc aacccgaccg
     1321 ccatgcccga tcaatcggta gaatacatcc tgcgtgcctc cgactcctac gggtgttctg
     1381 tttcagatac cattttcttt gacgtgcaat cgcagttgcc ggcgcccgaa ctcatctgcg
     1441 gcacggctac caacaactcc gtttctgtgg cttggcaaga cattcccggc gctgcgggtt
     1501 atgaaatcaa catcaacaac acgggctgga ctaccccttc cgacgcattg agccatttga
     1561 tagatggcct gagcgaaggg caaacctttg aggtcatgct gcgcgcactg ggtatgtgcc
     1621 ccggccctgc cgatacgctt caatgtcagg cgcttacctg cgctccaccc gatctgagcg
     1681 cgacggtctc ggatgcctcc tgtgcaggat tgtcaaacgg cggcgtgacg ctgagtacgg
     1741 cttccggtac cggaacgctg acttttgagt tgaacaacca aaccaacaac acgggcactt
     1801 tcagcggcct cgcgccgggc ttgtatgtag cttcgatgac cgacggcgcc aattgcccgg
     1861 ctacggtgca gttcaccatt tcggcgccac aggccatggc gctcagcctg tcattggcag
     1921 actcggtggc ttgtttcggg caaagcaacg gctcggcttc ggtggcggtt gcaggcggca
     1981 catccaatta cacttttgcc tgggacaacg gcgaggcaac agctacggcc acacaacttg
     2041 acgcaggcac gcacaccgtc accgtgacgg atatggccgg ctgtgtggcc gaatctgaca
     2101 ttatgattcc tcaaccggct gcgctttccc tttcggtaaa tgccaccgcc gtcgtctgtc
     2161 cgggagcggg caatggaacg gctaccgttg tgcccgcagg aggcacggcc tcttaccagt
     2221 ttgtctggga tgcccttgcc ggtaatcaaa atggcgctac ggcgcaaggt ttgagtggcg
     2281 gtaattatga ggtcatcgtc acagatgcca atggttgcac ggcccagatc agcactacag
     2341 tggccgagcc ggcagccata caaatgacaa cacaaacaac agatgctcaa tgctttggaa
     2401 ccgccaccgg cacagcctcc gcgacggcaa cgggcggcaa cggagctttc agttttgtat
     2461 ggagcaacat gcaaaacacg gcagcggcca cagggctgag tgccggaaat tacaccgtaa
     2521 tcgctaccga tgcagccggc tgccgcgata ccgctgcggt gcaggtcaat cagcccacag
     2581 cgctacaggt gcaatggcaa cctgccgacc ccgcttgctt cggcgatgcc gacggctcgg
     2641 cttcggcgac gatttccggc ggcacgggca tttnnnnnnn nnnnnnnnnn nnnnnnnnca
     2701 ggcgcccaac aaccatccgg ttcggcaaaa cctgagtgca ggcagctacg tcgttaccgt
     2761 cagcgatgcc aataactgca ctgaaacaca aaccattaca ctcgtcaatc cgccattgct
     2821 gagcgcggcc atgacgacct cgcccgccga ttgcaatgct gcggctaccg gttcggcgca
     2881 agcaacagcc acgggcggaa ccggcgctta cacctacctc tggtcgaacg ggcaaaacgg
     2941 ccccgacgct gcgaatcttc aggccggccc catttccgtc gtcgtcaccg atgccaacgg
     3001 atgcacgacc ttggcgacag gatcaatcac ggagccaaca gctatgaccc tgagcatcgc
     3061 gcccgctgat gcgcgctgtt tcggcgcttc cgacggcaca gccacagcaa tagccggcgg
     3121 cggagcaggc aattatacct acctctggag caatggccaa accgggcctg ctgcatcggg
     3181 gctgggtgcc gggacttata atttggtggt aagagacggc aacaattgta tgatctcgca
     3241 atcattcaac atctcccaac caacggcact gaatgccaca acgcaatctg taacagccag
     3301 ttgcagcccg caacccgacg gtgaagcctc tgtgtcggcc accggaggca cacaacctta
     3361 cacttatcgc tggagcgacc cggcccaaca aaccgacgct gcggcccgcc aactcccgcc
     3421 cggcgcgtac accgtcaccg tgacggacgc caacggttgc agctttacac aaaacgctgc
     3481 cgtagcaggc tccccggcag tgcagttgca attgcaggcc acgcccgtag gctgtcatgg
     3541 cggcaacaac ggcggcattt cggcgacggc taccggcggc acaggcgctt ttacctatca
     3601 atggagcgct gccggtttgg gcaatgtttc caaccccgcg cagctcagtg caggcttgta
     3661 ttcgctcacg gtgaccgatg agcggcagtg tacgggcatt tcgcaagccg atgtgacgca
     3721 accggcgccc ctcacactca gcgctgccac gcagcatgtg gcctgtgccg gcaatgccac
     3781 cggcggcatt gatctgacgg tgtcgggcgg tacgatgccg tatcgttttg cctggagtac
     3841 cggcgacaat gtggaagacc tttcccagct cagctccggc gtcttttcag tgacggtaac
     3901 ggatgccaac ggctgtgtgg cgaatttgga aacaaccgtc attcagacca cccgcatcac
     3961 ggcgagtttc agcacggtga gagccgattg ctacggcgcg gccacgggtg cggcctcggt
     4021 gttcgtcaac ggcggttcgg cgccttatct ctacgaatgg agcaacggcg gctcaggcgc
     4081 tgccattcaa aatgtgcctg ccggattgta ccaattggcc gtaaccgacg gcttgggatg
     4141 ccgcactgag ttcgaggtcg caatagaaca accggagcag cctctgaccg ccagccccaa
     4201 cctcatgccg cccacctgcc acgacgaaga caatggccgc atagaactga ccactaccgg
     4261 tggcacgcct gcgtatgaat acagtatgaa tggcggccct tttgtgggca accgcatttt
     4321 tgtggcgctc catgccggcg tctataccat tgccatcaaa gatgccaaag gatgtctttt
     4381 caataccggc ccgctgcaac tcgctgaccc tgcaccgctc agcgttgatc tcggcccgga
     4441 tgtgaccctt caatatggcg attctctgcg cattcagcct cgtattctgg gcgctgtgga
     4501 gccattgatg ttggaatgga agccccgcga tactttgttt ttcggatgcc tgagttgccc
     4561 cgaaccggtg atacggccct tgttccagcg agctatcacg ttggtcgtca cggatgccaa
     4621 cggctgcacg gcggaagact tgtttacggt gtttgtcgtc aaagacccgc gcgcctttgt
     4681 tcccaccggt ttcacgccca acggcgacgg catgaacgac cgtctgctgg tacacggccg
     4741 gcaaggtacc cgtgtgttgt cgttccgcat tttcgaccgc tgggggcagt tgttgtatga
     4801 aggcggcgat tttgaggtca acgattctgc ttttggttgg gacggcactt tccggggcaa
     4861 acccgtaacc gccgacacct acatctggtc gctgacggtg gagttgcagg acggagacaa
     4921 agagatactc tccggggaaa ctaatgtaat tagataacgg ctagcaatac aaatacggcg
     4981 ctttatgaaa agcatctggt ggatggtttt ggcttctgtt tgggtatggc cgttggcagc
     5041 acagccggtt gccgagcaaa aactgaacat cgcttttcgc aatgccggct tagaggcagc
     5101 cctcgagcag ctttccgaaa aatcgggcgt atcgttctcg tacagcagcg aggcattgnn
     5161 nnnnnnnnnn nnncgatgtg cgcatcacgg ctcgttttca gcgccaaaca gccctgcaca
     5221 ttctcaaata cctgctcacc gatacgggcc tcggctggcg cgtagtgggc aatcaggtag
     5281 tcatttacaa attgccgcct gtgccggagc gcaaatacac catcagcggc tttttgtacg
     5341 atgcttcctc cggagagccg ctcatcgggg cttggctgat ggaccaaaac tccaaaaagc
     5401 cggcagtgac caatgtgtat ggttttttca gcctcacgtt gccggcgggc gaggtgaacc
     5461 tgctgtgttc ttatgttggt tttgagcaaa aagacaccgc cttcacactc aaaaaagatg
     5521 tcgctttggt gtttcatctg cgcagcgcca ttacgctcac ggaggtggtg gtgaaggctc
     5581 gttcgggagc atcttcagcg ccgcagccac aaacgcaact attgggcaca gagatcgtgg
     5641 aagacttgcc ggcattgggc ggagaggccg acctagtgcg cggattgcat ttgttgccgg
     5701 gcgtgcaaac cggcaccgac ggcgtggaag gcttgcatgt gcgcggcggc tcgcaggggc
     5761 acaatttggt gctgatagac ggggtgccgg tttacaacta cggccacgct gcgggtattt
     5821 tttctgtgtt caacacggag gctgtgcgca gtgtgcgctt tctcaaaggc agttttccgg
     5881 cccgatacgg cggctggctt cggtgataga cgtgcgcacc aaagagggca atacccgaca
     5941 ctttcagggc cgcgccgaaa tgggcatgct ctcctcgcgc ctcacgctgg aggggccgat
     6001 tgtacgagac aaaagcgcgt tttttatttc cgggcgtacc tcgctcatca actggtatct
     6061 gaggccgctg tcgcggaatt tcaaaacaga acagggcgag cagggtcaga ctttgtacaa
     6121 ttttgacgat atcaacgcga ag
//
LOCUS       DAORLF010000292         6133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6944_length_6133_cov_1.462488, whole genome shotgun sequence.
ACCESSION   DAORLF010000292 DAORLF010000000
VERSION     DAORLF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_6133_cov_1.462488"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>303)
                     /locus_tag="PK198_19160"
     CDS             complement(<1..>303)
                     /locus_tag="PK198_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40922.1"
                     /translation="PTIVFFDTLRVQVKAEETAELCLRLINPSQENATTVEVVIEGSP
                     APYFGEAEALRSVEFAPGETRQCLSYLPPRVEEADSITLYTLRLQNAAGGHAVAIGE"
     assembly_gap    304..313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(368..1093)
                     /locus_tag="PK198_19165"
     CDS             complement(368..1093)
                     /locus_tag="PK198_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40923.1"
                     /translation="MQQPGAYSVVLTHNIYGCSDTLSTNVSAAPVWTQSIEASSDFLC
                     DEVRDIKLNVNGTSGGSYSYAWSGGSLTEQQILTTTGVYYLTLTDDATGCSTVLSRVV
                     QNSDSDIALRIEAPHTLLCRDGEMTLRAVLDGLHESLRERVRLEWGHHGSEQVTTDIR
                     TPDRYGVTAFFGEHCRQETMIEILQAPDLEVRIQSPEGTKHACPESPLLLQAVVSDST
                     LVAAYHWSGGADTPEAVFTESGP"
     gene            complement(1053..>3392)
                     /locus_tag="PK198_19170"
     CDS             complement(1053..>3392)
                     /locus_tag="PK198_19170"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40924.1"
                     /translation="ITPSPHRATTVALAFNNETSPHFSSAPSPVLVTFPAGSSERQCV
                     DIPSALWPGDDPRSQYSLHIQSVTGGIYPVVGPDTERTLRVRDANTCWSTVQQQNFSA
                     ADQNLSNVSGWVANNGAARSGYGATGQEFDYCAAMTMTPGSYAGFQQWLESGKQYRVR
                     WNLKVDAKKPASVFLSLGTQAGIGNKVGVAVSIAPSGNQQPGIEFTSEYFTVPANGNY
                     FLLLNAEIGANPIVRFDDFRLEARCPDTEVFFQQSLVETAEGNALLVCVQIANPSPSS
                     PTTVEVSFEGLQSPHFNGVPATRTVTFPAGSSTPQCLLFPTTANGQYDGGAVEYILRL
                     QNITGNGARIAQPDRATLRVLDDVSLENCFWAGPDRQICLGQSTQLGLPGAAMPAHCS
                     AAPFCFRWEPASLILNGGQYDARPFVAPQQDTWFVIYMTDGHGNLHSDSVLVQVTASL
                     QASITGHETPVCSGGARTLQVQTSGGAGSLSYQWQHSMDGGASWAVIAGVTGSVYTSP
                     FLSASRMYRVAAGSSGCGSVVTAPATVQVLPDPSLSISASAASVCLGGFSLLSASPAN
                     GTGACTTQWQSSSSASGPWNNIAGATGNSLAPSTLVPGIRYFRASYTCSGGGCDVAFS
                     NVVSVTVVPGPSISTCRRYHLLRQHAHPGRGRHRRHARPELPMAKQPQRHDLVHPFWR
                     YAIEPDDPAADKQYLLPRSSERKRQRLRHGVFASGLSAGHCPARLVHSPRAGGTLPRH
                     LADTQRARCAGRLVFVARRLHCAPMGGAAARRLQRRTHP"
     assembly_gap    3393..3402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3403..>4602)
                     /locus_tag="PK198_19175"
     CDS             complement(<3403..>4602)
                     /locus_tag="PK198_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40925.1"
                     /translation="NNVHYFGLKVTLEAGYNYRLKWAGKSGGFAKSVQVHFGASTTST
                     EVVGNSHTMPLLNPSMPAPAFTSQSFTPPSNGQYYVLLKLAPGSIGSGSLRFDDLVLE
                     RSCSVIAPPAVFFTTQDAEAAEGGSVEICVGIQTPYIYHATQVQVAAAGPVQPHLASF
                     STQTLTFPAGSAQNRCFTFFIPANNEPDGDFSYRFVLQNPSSSGGNPYTAQPDTLILS
                     VKDDILQNNCANWNPGTIYNLDTDMPGNWQHGAPSSLSTDWADYAGDYAYKTDYSTAA
                     YIAIPFQLSPLFSYRLQWKGRAQNPAIPEILLMRGTAPNSGVLMPVAQQADGAWGIYT
                     GEAFEVATAGTYYLTIKGGGTFFNLKTDLYIDDIVLQRVCKTTSVAFSSITPQIVEGD
                     EGALFRVC"
     assembly_gap    4603..4612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4613..4876)
                     /locus_tag="PK198_19180"
     CDS             complement(<4613..4876)
                     /locus_tag="PK198_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40926.1"
                     /translation="MLLRSVSRLFVCLVLPLQAMALHASPSNDCWNVAMSESFSPSNY
                     NNFSNLPHIANNGFSRAEYGSSGAFSDVCALVPGMISKRGSKST"
     assembly_gap    4979..5061
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5201..5431
                     /locus_tag="PK198_19185"
     CDS             5201..5431
                     /locus_tag="PK198_19185"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRF40927.1"
                     /translation="MNFKERIDSLRRLHLLILRKATGTPDELARKLGISPPSLFRHIE
                     ILKEFGAPVAFCRTHQRYYYTEDFELDFNRKF"
     gene            complement(5444..>5754)
                     /locus_tag="PK198_19190"
     CDS             complement(5444..>5754)
                     /locus_tag="PK198_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40928.1"
                     /translation="FLQRVINGGGRIEREMAIGNGRTDLAVFWKEQVIPIELKMHHDK
                     WSQPEGIEQLGRYMDKLGQKQGYFILFEKKSSEELPWETRIRREVHEVDGKEVILLGM
                     "
     assembly_gap    5755..5909
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5910..>6133)
                     /locus_tag="PK198_19195"
     CDS             complement(<5910..>6133)
                     /locus_tag="PK198_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40929.1"
                     /translation="THLDSLADKIQEERVRRVLLGIITGEAPAFDGWDDSVRYCRDLG
                     IISYGNPIQFANPIYREIITRIINSGFADN"
BASE COUNT         1220 a         1514 c         1832 g         1299 t
ORIGIN      
        1 ttcgccgatg gcgacggcgt gtccgccggc tgcgttttgc aggcgcaggg tgtagagggt
       61 gatgctgtcg gcttcttcta cgcggggtgg caggtagctg aggcattgac gggtctcgcc
      121 gggcgcaaac tccacgctgc gcagggcctc cgcctcgccg aaatacggcg ccggactgcc
      181 ctcgatgact acttccaccg tagtggcgtt ttcctgcgag gggttgatca ggcgcaggca
      241 cagttccgcc gtttcttcgg ccttcacctg cacgcgcagg gtatcaaaaa atacaatggt
      301 aggnnnnnnn nnncacatcg aagcggtctt cccacacaca tccgtttaca tcggtgacga
      361 ggagggtcta tgggccgctt tcggtaaaca cggcttcggg cgtatcggcc ccaccgctcc
      421 agtgatacgc ggctaccagc gtactgtcgg agactacggc ttgcagtagc agcgggcttt
      481 ccgggcaggc gtgcttcgta ccttccgggg attgtatgcg gacttccaga tcgggggcct
      541 ggaggatttc aatcatggtc tcttgccggc agtgttcgcc gaagaaagcc gtcacaccgt
      601 atcggtcggg cgttcgaatg tcggtggtaa cctgctccga gccgtggtgc ccccattcca
      661 agcgcaccct ttcgcgcagg ctttcgtgca gaccgtcgag tacggcgcgc agggtcattt
      721 cgccgtcgcg gcacaggagg gtatggggcg cttctatgcg cagcgcaatg tcggaatcac
      781 tgttttgcac aacgcggctc agcacggtgc tgcatccggt ggcatcgtcg gtgagggtga
      841 ggtaatagac gccggtggtg gtaagtatct gttgttctgt aaggctcccg cccgaccagg
      901 cgtaggaata gctgccgccg gaagttccgt tcacgttcag tttaatgtcc cttacttcat
      961 cacaaaggaa atcggaagaa gcctctatgc tttgggtcca gacgggggcg gcgcttacgt
     1021 ttgtcgagag cgtatcggag cagccatata tattatgggt gagtacgacg ctgtaggcgc
     1081 cgggctgctg cacctcccat tggggcgcag tggagccgtc gggccacaaa aacgagacgc
     1141 cctgcgcatc gggcacgctg agtatcagcg aggtgtcggg gcagagtacc gcctgcgcgg
     1201 gggctatgga ccagtcgggc agggcaatga cctgcactaa ggccgcttgc gaatacaccg
     1261 tgtcgcagcc gctgccgctt gcgctcactg ctacgcggta gtaggtattg cttgtcagcg
     1321 gcggggtcgt caggctcgat tgcgtatcgc caaaatgggt ggaccaggtc gtgccgttgg
     1381 ggctgctttg ccattggtag ctcagggcgg gcgtgcctcc ggtggcggcc acgtccaggg
     1441 tgcgcgtgct gccggagcag atggtagcgc cggcaggtgc tgatagaggg gccgggtaca
     1501 accgtgacgg aaacgacatt ggaaaacgcc acatcgcagc cgccgcccga acaggtgtag
     1561 gaagcgcgga aataccggat gcccggcaca agggtagaag gggcaaggct gtttccggta
     1621 gcgccggcga tgttgttcca ggggccggaa gccgaagaac tgctctgcca ttgcgtggtg
     1681 caggcgccgg taccgttggc agggcttgcg ctcaaaagac tgaatccgcc caaacaaacc
     1741 gaagcggccg aggctgagat gctcaggctg gggtcgggga gtacctgcac cgtagcgggc
     1801 gctgtgacga cgctgccgca tccgctgctg cctgcggcta cccggtacat gcgggaggcg
     1861 ctgaggaagg gcgaggtata aacgctgccc gtgacgccgg caatgacggc ccaggatgcg
     1921 ccgccgtcca tgctgtgttg ccactggtag ctgaggctgc ctgcgccgcc gctggtttgc
     1981 acctgaagcg tgcgcgcgcc gccggagcag acgggcgtct catgtccggt gatggaagcc
     2041 tgcaaggagg cggtaacctg caccaataca ctgtcggagt ggaggttgcc gtggccgtcg
     2101 gtcatataaa tcacaaacca ggtatcctgt tggggcgcta caaaaggccg ggcatcgtat
     2161 tgcccgccgt tgaggatgag ggaggcgggc tcccagcgga aacagaaagg cgcagcgctg
     2221 caatgggcgg gcatggcggc gccgggcagg ccgagttggg tgctttgccc caggcatatt
     2281 tggcggtcgg ggccggccca gaaacagttt tcgaggctca catcgtccag cacgcgcagg
     2341 gtggcgcggt cgggctgggc tatacgggcg ccgttaccgg taatgttttg cagccgcagt
     2401 atgtactcta cggcgccgcc gtcgtattga ccgttggcag tggtgggaaa cagcaggcat
     2461 tgcggggtgg aactgccggc cgggaaggtg acggttcggg tggcaggtac gccgttgaaa
     2521 tgcgggcttt gcaggccctc aaagcttacc tccaccgtag tggggctgct tggggagggg
     2581 ttggcaattt ggacgcagac caacagcgca ttaccttcag ccgtttccac caggctctgc
     2641 tggaaaaaga cctctgtgtc ggggcaacgg gcttcgagcc tgaaatcgtc gaagcggacg
     2701 atggggttcg ccccaatttc ggcattcagg aggagaaaat agttgccgtt ggcgggtacg
     2761 gtgaagtatt cggaggtaaa ttctatgccg ggttgttggt tgccggaagg cgcaatggaa
     2821 acggcaacgc ctactttgtt tccgataccg gcttgtgtgc cgaggctcag aaaaacggag
     2881 gcgggctttt tggcatccac tttcagattc caccttacgc ggtactgttt gccgctttcc
     2941 aaccattgtt gaaatcctgc atagctgccg ggcgtcatgg tcatggcggc gcagtagtcg
     3001 aactcctgac cggtagcgcc gtaaccactt cgggcagccc cgttgttggc cacccatccc
     3061 gaaacattgc tcaggttttg atctgccgca gagaagttct gctgttgtac ggtactccag
     3121 caggtgttgg cgtccctcac gcgcagggtg cgttcggtat ccggcccgac gacaggataa
     3181 atgccgccgg tcaccgactg aatgtgcagg ctgtattgcg agcgcgggtc gtcgccgggc
     3241 cagagtgcgg aaggaatgtc cacgcattgc ctttcagaag aacctgccgg aaaagtgact
     3301 aagacagggg agggcgcgct ggaaaaatgc ggcgaggttt cgttgttgaa agccagagca
     3361 accgtagtgg cccggtgtgg agagggcgta atnnnnnnnn nngcaaaccc tgaacagggc
     3421 gccttcatcg ccttccacga tttggggcgt gatggaggaa aaagccacag aggtggtttt
     3481 gcatacgcgc tgcagcacaa tatcgtctat ataaaggtcg gttttcagat taaaaaacgt
     3541 gccgccgcct ttgatggtca ggtaataagt gcccgcagtt gccacctcga aggcttcgcc
     3601 ggtgtaaatc ccccatgcgc cgtcggcctg ttgggcaacg ggcatcaata cgccggagtt
     3661 gggagcagta ccccgcatca gcaaaatttc ggggatggcc gggttttgag cgcgcccttt
     3721 ccattggagg cggtaggaaa acaaggggct gagttgaaaa ggaatggcta tataagcggc
     3781 agtggaatag tctgtcttat aggcatagtc tccggcgtaa tcggcccaat cggtactcag
     3841 gctgctgggg gcgccatgtt gccagttgcc gggcatgtcg gtgtctaagt tgtatatagt
     3901 gcccggattc cagttggcgc agttgttttg caatatgtcg tcttttacac tcagtatgag
     3961 cgtgtcaggc tgggcggtgt agggattgcc gcccgaagag gaggggtttt gaaggacaaa
     4021 tcggtagctg aaatcgccgt cgggttcgtt gttggccgga atgaaaaatg taaaacaccg
     4081 gttttgtgcg cttcctgccg gaaatgtgag ggtttgagtg gaaaatgaag ccaggtgagg
     4141 ctgtaccggc cccgctgcgg ccacctgtac ctgagtggca tgatatatat aaggcgtctg
     4201 tatgccgacg cagatttcta ccgagccgcc ctcggcagct tcggcatcct gggtagtaaa
     4261 aaacactgcc ggaggggcaa tgacggaaca actgcgctcc aataccagat cgtcaaatcg
     4321 gaggctgccg gaaccgatgc ttccgggagc aagtttgagc aggacgtagt attggccgtt
     4381 ggagggcgga gtgaatgatt gcgaagtgaa tgccggggca ggcatacttg gattgagcag
     4441 cggcatggtg tgcgaattgc cgacaacctc cgtactcgtt gttgaagcgc cgaaatgcac
     4501 ctgcacggat ttggcaaagc cgcccgattt gcctgcccat ttcaggcggt agttgtagcc
     4561 ggcttccaac gtcactttca acccgaaata atgtacattg ttnnnnnnnn nnggtcgatt
     4621 tcgacccgcg cttcgagatc atgcccggaa ccaaggcgca gacatccgaa aatgcgcccg
     4681 aactgccata ttccgcccgc gagaagccgt tgttggcaat atggggcagg ttggagaaat
     4741 tgttgtagtt ggaagggctg aaactttcag acatcgccac attccagcaa tcgtttgatg
     4801 gagaggcgtg cagggccatt gcctgtaaag gcaaaacaag gcatacaaat aaccggctga
     4861 cagaacgcaa caacatattc agtttttttc tggtgtgtat cgaataagaa acggagcaaa
     4921 cttagggggt gcaggtatca aaaattgaga gttaggaaaa aaagatttgc atattattnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nttgttgtcg cttttttcat caatgtgttt attatttcag
     5101 cccggtgtag tgaagtattg cttcataaac tccgaactca acgcctcata tcgtaaaacg
     5161 cgaatgattt catttacaca cttttaagtt ctaacaaatt atgaatttca aagaacgaat
     5221 cgactcctta cgtcggctgc atttgctgat tttgcgcaaa gccacaggaa ctccggatga
     5281 attggcccgt aaattgggaa tttctccgcc ctccttattc cggcacattg aaattttgaa
     5341 ggaattcggg gcgccggtcg ctttttgccg aacccaccag cgttattatt acacggaaga
     5401 cttcgagttg gattttaacc ggaagttcta aggggatgcg gggtcacatc cccagtaaaa
     5461 tcacttcttt gccgtccacc tcgtgcactt cccggcggat gcgggtttcc cagggcagct
     5521 cttcggatga cttcttttca aagagaatga agtagccctg cttttggccc agcttgtcca
     5581 tgtagcggcc taattgctct atgccttcgg gctgcgacca tttatcgtgg tgcattttca
     5641 gttcgatggg aatgacctgc tctttccaaa aaacagctaa gtccgttcgc ccgttgccga
     5701 tggccatttc tcgttctatg cgtccgcctc cgttgatgac gcgttgcaaa aaagnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttgtcggcga aaccgctgtt gatgattcgg
     5941 gtaatgatct ccctgtaaat gggattggca aactgtatgg ggttgccata agaaatgatg
     6001 cccagatcgc ggcaatatcg aacggaatca tcccatccat cgaaggccgg cgcctctcct
     6061 gtaataattc ccagcaacac ccgtcgcaca cgttcttcct gtattttatc ggccagactg
     6121 tccaagtgcg ttt
//
LOCUS       DAORLF010000293         6111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_6991_length_6111_cov_2.030053, whole genome shotgun sequence.
ACCESSION   DAORLF010000293 DAORLF010000000
VERSION     DAORLF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_6111_cov_2.030053"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>234
                     /locus_tag="PK198_19200"
     CDS             <1..>234
                     /locus_tag="PK198_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40930.1"
                     /translation="GGSLVMPIPWLEVLKIGSVVTAAIIFFPLLSMILRAVRGISRPQ
                     EQRPGFKLWTWFFFWSIITHPLLDACTTYGTQL"
     assembly_gap    235..270
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <271..>614
                     /locus_tag="PK198_19205"
     CDS             <271..>614
                     /locus_tag="PK198_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40931.1"
                     /translation="NNISVADPAYTFPFLLFVIIASRMAKTGRARRIFNWIGIGISSA
                     YMMFTFYNKVKVDGIFERSLQRENIAAQRFMTSPTILNNILWQGVAEGDSVYHHGMYS
                     LLDARPEVDSFV"
     assembly_gap    615..642
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <643..920
                     /locus_tag="PK198_19210"
     CDS             <643..920
                     /locus_tag="PK198_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40932.1"
                     /translation="IRPYADERPIRVLRWFSDDYYTVLRRKDGRLQFNDLRFGSMTGR
                     FDTETDLVFGFIIEEKDGQLLVTGANERPDAAGDSFRQLWLRMLGRN"
     gene            970..2064
                     /locus_tag="PK198_19215"
     CDS             970..2064
                     /locus_tag="PK198_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRF40933.1"
                     /translation="MTSIFTKTMRWWMPVVMLCGFISNATAQKYSNEFLSIGVGARAQ
                     GMGMAVIAGGSDVTSGVWNPAGLAGIDLPNGFQLGAMHSEWFGGVGKFDYAGVVLPLA
                     NNHRRLGLSFIRFGIDQIPNTLTLYEDDGTVNYDNVSEFSAADYAFVLSYAQLVKPEV
                     GKLAVGGNIKIVHRRIGPFATSWGFGADLGLQYRLNKWRLGVLARDITTTFNAWAFSF
                     TDEQKEVLSLTNNEIPINSVEITRPQIILDITRRFEWKNVGISPEIDLIATTDGRRNT
                     LISAAPFSIDPAIGLELDYKQKVFLRGGASQFQREMDFGGKELLGVRPAIGVGLKVGA
                     LNLDYAFTDLGSQQNTFSHVVSVMLNVRRR"
     gene            complement(2083..2424)
                     /locus_tag="PK198_19220"
     CDS             complement(2083..2424)
                     /locus_tag="PK198_19220"
                     /inference="COORDINATES: protein motif:HMM:NF020444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XisI protein"
                     /protein_id="HRF40934.1"
                     /translation="MEKIALYKKIVRELFVEIAAMSPSDESVQTQLITDDERGHYLLF
                     SVGWEDDYREYTPFLHIDVNDQGRVLIQHDGTDLKVALLLAEKGIPKSDIVLAFHPLY
                     QRPLLPDFAIA"
     gene            complement(2412..>2694)
                     /locus_tag="PK198_19225"
     CDS             complement(2412..>2694)
                     /locus_tag="PK198_19225"
                     /inference="COORDINATES: protein motif:HMM:NF020393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="element excision factor XisH family protein"
                     /protein_id="HRF40935.1"
                     /translation="VGAERENEKIAVEIKSFVSLSDMDAFEEALGQFLLYRPALAQKE
                     SDRTLFLAIPNGFYTKFFDDPFFMEIADLYQLKLLIFNEKESIITQWKK"
     assembly_gap    2695..2795
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3080..3150
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3356..4867
                     /locus_tag="PK198_19230"
     CDS             3356..4867
                     /locus_tag="PK198_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF40936.1"
                     /translation="MLYLAGFIGTVTTLAAWLLLKARGRSVRGLPGGFIIALGAYLIG
                     LLFSGVGLEAKLGVFARDMLVVAVSGIAVMAMVARRKALGPGLLALGLLLAWFVQFQL
                     RPTMQQAAERIELDADGELLIQISEGRQVKELNALIQEYGLDIQPAFSPASPEITQLD
                     NYYVVNVPTAQEGKLGRIKKSLHRSGMIDWLEENEVVTVAPMPAQNPAPIQRRYGIDD
                     PGLSQLWGFEAMQVDQLYETLRKGNIQPRKKALIAILDTGVDAQHEDIKANYRSLSAA
                     YDNDPMKHGTHCAGIAASVSNNGLGVASFSVDNAFFQVASIKVLNAGGSGTQQGIIRG
                     MIEAADAGADVISMSLGGRTGSVKQTAYEKAVKYAAKKGAIVVAAAGNSNRNAKDFAP
                     VNTPGVIGVSAVDAGLNRAVFSNFVNDIPWAVAAPGVDIYSTVPGNQYASFNGTSMAT
                     PYVAGLLGLLKSIRPELTAREAYNLLQSTGKETKNTRETGRLIQPAKAVGGLR"
     assembly_gap    4914..5029
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5030..>5644
                     /locus_tag="PK198_19235"
     CDS             <5030..>5644
                     /locus_tag="PK198_19235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRF40937.1"
                     /translation="VAVGIGIVLMGVKFFAWWLTNSNAILTDALESIINVVAGLFALY
                     SLHITSIPKDANHPYGHGKVEFMAAGFEGGLIVLAGLLIVGKGAYNLIFPQELEKIDI
                     GLILTAFSGLINFGLGFFVLRQGKKSHSLTMQADGKHLLSDAYSSAGILLGMLLLYFT
                     GATWLDNLTAIIFGFVILYTGLQLLRTSVSGIMDEAEPETIRPLV"
     assembly_gap    5645..5799
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5800..5993
                     /locus_tag="PK198_19240"
     CDS             <5800..5993
                     /locus_tag="PK198_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRF40938.1"
                     /translation="LETLVQKDNPTQVELFVHADPCRPPSACSICRKADCAQRQAAFE
                     RDVPWTLESLLHLRNHQEL"
BASE COUNT         1307 a         1453 c         1449 g         1395 t
ORIGIN      
        1 cggcggttct ttggtgatgc cgatcccctg gttggaggtg ttgaaaatcg gttcggttgt
       61 cacggcagcc atcatttttt ttcctttgct cagtatgatt ttgcgggcgg taagaggcat
      121 ttcgcggcca caggaacagc gccccggctt caaattgtgg acttggtttt tcttttggtc
      181 catcatcact cacccgctat tggacgcctg caccacctac ggcacccaat tgttnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacaacatc tccgtagccg atccggccta
      301 cactttcccg tttctgctgt ttgtcatcat cgcctcccgg atggccaaaa caggccgcgc
      361 ccgccgtatt ttcaactgga tcggcatcgg catcagcagt gcctacatga tgtttacttt
      421 ttataacaag gtgaaagtgg acggtatttt cgagcgttcc ctgcaacgag aaaatattgc
      481 agcacagcgt tttatgacct cgcccaccat tctcaacaac atcctgtggc agggcgtggc
      541 agaaggcgat tcggtgtatc atcacggcat gtactcgttg ttggatgccc gtccggaggt
      601 ggactctttt gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnggattcga ccctacgccg
      661 atgagcggcc gatacgagta ttgcgctggt tttcagacga ctactacaca gtgctgaggc
      721 gaaaggacgg tcgcttgcag ttcaacgacc tccgtttcgg ttctatgacg ggccgttttg
      781 atacagaaac cgacctcgtt tttggcttta ttattgaaga aaaagacggc caactgttgg
      841 tcaccggcgc caatgaacgc cccgatgctg cgggagattc attccggcaa ttgtggctgc
      901 ggatgctggg ccgcaactga ggcaataccc gccttattcc tacgtttcca ttttcaaaaa
      961 aagcagaaaa tgacttccat ttttacaaaa acgatgcggt ggtggatgcc ggtggtgatg
     1021 ttgtgtggct ttatttcaaa cgcaactgca cagaaataca gcaatgaatt tctctctatc
     1081 ggcgtgggag ccagagcgca gggcatgggc atggccgtta ttgccggcgg ttcggacgtg
     1141 acttccggcg tatggaatcc ggctggactg gcgggtattg atctgcccaa cggctttcag
     1201 ttgggggcca tgcactccga gtggtttggc ggcgtgggca aattcgacta tgcaggcgtg
     1261 gtgctgcctt tggccaacaa ccatcgccgt ttgggcttgt cattcattcg tttcggcatt
     1321 gatcagattc ccaatacact caccctttat gaagacgacg gcacggtgaa ttacgacaac
     1381 gtgagcgagt tttcggccgc cgattatgcc tttgtgctga gttatgccca gttggtcaag
     1441 ccggaagtcg gtaaattggc tgtgggcggc aacatcaaaa tagtacaccg gcgcatcggg
     1501 ccgtttgcta cttcctgggg tttcggcgcc gatctgggtt tgcaataccg gctgaacaag
     1561 tggcggctgg gcgtactggc ccgcgatatt accaccacgt tcaacgcctg ggcattcagc
     1621 ttcaccgacg agcaaaaaga ggtactctcg ctgaccaata atgaaatccc catcaacagc
     1681 gtggagatca cccgaccgca aattatattg gacatcaccc gccgttttga atggaaaaac
     1741 gtaggcatct cgccagaaat agacctcatt gccactaccg acgggcgacg caatacgctc
     1801 atttcagcag ccccgttcag catagacccc gccatcggat tagagttgga ttacaaacaa
     1861 aaggtcttcc tgcgcggcgg tgcgagccag tttcagcgcg aaatggattt tggcggcaag
     1921 gagttgctgg gcgtacggcc ggccatcggc gtgggtctga aagtgggcgc actgaatctg
     1981 gactacgcgt tcacggattt aggctcgcag cagaacacgt tttcgcacgt tgtttcagtg
     2041 atgctgaatg tgcggaggag gtgagttggg gatacaaagt aatcaggcta ttgcaaagtc
     2101 ggggagtaaa ggccgttgat atagggggtg aaacgcaaga acaatgtcgc ttttaggtat
     2161 gcctttctcc gccaacagca gtgctacttt caggtcggtt ccgtcatgtt gaatcaaaac
     2221 ccgcccctga tcgtttacgt caatatgcaa aaaaggtgta tattctctgt agtcatcttc
     2281 ccagcctacg gaaaaaagca ggtaatggcc ccgttcgtcg tctgtaatca attgtgtttg
     2341 aacactttcg tctgagggcg acatagctgc gatctctaca aagagttcac ggacaatttt
     2401 tttgtaaagc gctatttttt ccattgtgta attattgatt ctttttcatt gaaaatcagc
     2461 aacttcaatt gatacaaatc tgctatttcc ataaaaaacg gatcgtcgaa aaatttggta
     2521 taaaatccat tcggtattgc caaaaacaat gttctgtcgg attctttttg agctaaagca
     2581 ggtctgtaaa gcaggaattg tcccaatgcc tcctcaaatg catccatgtc tgataaactc
     2641 acaaaactct ttatctccac agcaattttt tcattttctc tttcggcgcc gacgnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaaag catgacggac tacatggtgg
     2821 aaaaaatctc tcgcgggcat taagatgtat ttatgtgcaa atatacgata atccgccaac
     2881 gcctgccttt gtactttctg tctatttttt aaaaaacact gctatgacca gaatcttatt
     2941 cctcgtcctt ttttgtgcaa tagcggcgac cgcagtcgtc tccgcacaaa caaaattcag
     3001 tatcggcccg gaagcgggca tctctgtgtc taccgaactc aacttcagac ccgttttccg
     3061 ccgccatgat gtgtttttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcaaaaccc gcagtacatc tgttgaaaaa
     3181 tagctttttt ggtcaaacat gactaaattg cccaagattt tcaaaggcag cgtttcattg
     3241 gtcatcgaat agaattaccc gatgctttct tgaacgctaa ccgtgagtaa tcagcacatt
     3301 atcacatcgt cacatcagca cattgattca tcttctcacc caaatacttc tgacaatgct
     3361 ctatttggca ggatttatcg gaaccgtgac cacgctcgca gcctggttgt tgttgaaagc
     3421 ccgcgggcgc agcgttcgcg gattgcccgg cggttttatc attgcactgg gcgcttacct
     3481 catcggattg ctattttcag gtgtggggct ggaggccaag ttgggcgtct ttgcgcgaga
     3541 tatgctggta gtggctgtgt ccggcatagc cgtgatggcg atggtggcgc ggcgcaaggc
     3601 attgggaccc ggcctcttgg cactgggcct gttgctggcc tggtttgtgc agtttcagtt
     3661 gcgccccacc atgcagcagg ccgccgaacg catagaattg gatgccgatg gcgaattgct
     3721 catccagatc agcgagggga ggcaggtaaa agaactcaat gcgctgatac aggaatatgg
     3781 ccttgacatt cagcctgcat tctcgcccgc cagcccggaa atcacgcaat tggacaacta
     3841 ctatgtggtg aacgtaccga ccgcccagga aggcaaactc ggccgcatca aaaaatccct
     3901 ccatcgctcc ggcatgattg attggttgga agaaaatgaa gtggtaacgg tggcgcccat
     3961 gcccgctcaa aaccccgcgc ccatacagcg ccgctacggc attgacgacc ccggcctgag
     4021 ccaactctgg ggatttgaag ccatgcaggt ggaccaactc tacgaaaccc tccgcaaggg
     4081 caatatccaa cccaggaaaa aagcgctcat cgctatttta gacaccggcg tggatgccca
     4141 acacgaagac atcaaagcca attaccgctc tctctcagcc gcctacgaca atgacccgat
     4201 gaagcacggt acgcactgcg caggcattgc ggcctctgtg tcgaacaacg gattgggggt
     4261 ggcctctttt tctgtggaca atgccttctt tcaggtcgcc agtataaaag tactcaacgc
     4321 gggcggctcc ggtacgcagc agggcatcat tcgcggcatg attgaagccg ccgatgcagg
     4381 ggccgacgtg atttccatgt cgctgggcgg gcgcacgggt tccgtcaaac aaacggccta
     4441 tgaaaaagca gtgaagtacg ccgccaaaaa aggcgccatt gtggtggccg cagccggcaa
     4501 ttccaaccgc aatgccaaag attttgcccc cgtgaatacg cccggcgtca ttggcgtgag
     4561 cgcggtggat gccggtttga accgggccgt tttttccaac tttgtcaacg atatcccctg
     4621 ggcggtagct gctccgggcg tggacattta ttccaccgtt cccggcaatc agtatgcgtc
     4681 gttcaacggc acctcaatgg ctacgccgta tgtggccggt ctgctgggct tgctcaagag
     4741 catccgcccg gaactgacgg cccgcgaggc atacaatttg ctgcaaagca ccggcaagga
     4801 aaccaaaaat acccgcgaaa ccggccgcct gattcaaccg gcgaaggctg tgggtggttt
     4861 gaggtagccg gttccaggga tgaggggtga gtgtgtgagc aacccggaac cttnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtagctgtcg
     5041 gcatcgggat cgtcctcatg ggcgtcaagt tttttgcatg gtggctcacc aattccaacg
     5101 ccattctcac cgatgctctg gaaagcatca tcaacgtagt cgcaggctta tttgccctgt
     5161 acagcctgca catcacatcc atacccaaag atgcgaatca cccctacggg cacggcaaag
     5221 tagagtttat ggctgccgga tttgaaggcg gcctgatcgt attagccggc ctgctcattg
     5281 tgggcaaagg cgcttacaac ctcatttttc cgcaggaact cgaaaaaata gacatcggcc
     5341 tgatactgac ggcgttttcg ggactgatca atttcggatt gggatttttt gtgttgcgcc
     5401 aggggaaaaa atcgcactcg ctcaccatgc aggccgacgg caaacacctg ctgtcagatg
     5461 cctattcctc agcgggtatt ctgttgggta tgttgctgtt gtatttcacc ggcgctacct
     5521 ggttggacaa cctcacggcc attattttcg gattcgtcat tctttacaca ggtttgcaat
     5581 tgctgcggac ttccgtctcc ggcatcatgg acgaggccga acccgaaacc atccgccctt
     5641 tggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttggaaacc ttggtacaaa
     5821 aagacaatcc cacacaggta gaactgtttg tacacgccga tccctgtcgc ccgccttctg
     5881 cctgttccat ttgccgaaag gccgactgcg cgcaacgaca ggccgctttt gaacgagatg
     5941 tgccctggac attggagagc ctcctgcatt tgagaaatca tcaggagttg tagttggggc
     6001 cagtatagtg aaggggggat ttaccctgta aatacaaaat agagtcaccc cttcattaag
     6061 tagtggggca aaaataaatg ctcattttct ttaggccaaa ggattagata a
//
LOCUS       DAORLF010000294         6042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7099_length_6042_cov_2.004009, whole genome shotgun sequence.
ACCESSION   DAORLF010000294 DAORLF010000000
VERSION     DAORLF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_6042_cov_2.004009"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..723
                     /locus_tag="PK198_19245"
     CDS             76..723
                     /locus_tag="PK198_19245"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF40939.1"
                     /translation="MDHQTMYYQKRAGEYEQIYQKPERQADLQQLKAYLSRQWMGKHI
                     VEIGCGTGYWTEILARHCALVTATDIHQEVIEQAKLKAYPRQNVQFEIMDFYHPERRQ
                     PRLEGLFGGFVWSHVPLQGLRDFLLLCLDQIEAGAELIFMDNQYVEGSSSPIHRTDEH
                     GNTCQLRTLKSGETFEIIKNFPDKISVQQSISDLCTDFEWLELKYFWAAKFKKPA"
     gene            complement(743..1585)
                     /locus_tag="PK198_19250"
     CDS             complement(743..1585)
                     /locus_tag="PK198_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40940.1"
                     /translation="MNRVLIIDSGATKADWSLMQAGELVSEFKTPGIPPFFMSDEEIS
                     AQLAVIAGQTGDTVDGIFFYGTGCKAESSRKRLHAAFSAAFPSAAAIEIETDVLGAAR
                     ALCGRQAGIACILGTGSNSVWYDGAQVLQNSGGYGYILGDEGSGAVLGRELIIAWLNR
                     ELPAELEQAFDAEYGLSRDAVIERVYRGETPSRFLAGFTPFLSAHLVSAFVQELLQRQ
                     FHLFVRRCVLPYPDARRLPVHFTGSVAFYFRGVLEGVLSEAGLQAGTFLAAPMEGLRG
                     FHRV"
     gene            complement(1582..2409)
                     /gene="murQ"
                     /locus_tag="PK198_19255"
     CDS             complement(1582..2409)
                     /gene="murQ"
                     /locus_tag="PK198_19255"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRF40941.1"
                     /translation="MQKLTEAPSHYRHLEQMPVAELLKGINQEDQTVPLAVEKALPQI
                     EKFVEALVEKMNNGGRLFYIGAGTSGRLGILDASEIPPTYGVADNWVIGIIAGGDTAI
                     RKPIEFAEDDPTQAWKDLKQHFINQQDVVLGIAASGTTPYVIGGLHDCRANGILTGCI
                     ASNAGTPLAAAADFPIEVVTGPEFVTGSTRMKAGTAQKLVLNMISTTLMIRMGRVLDN
                     KMVDMQLSNTKLVDRGAKILMNALQLDYPTAEALLLEHGSVRKALESRSGVESSIQV"
     gene            2723..3406
                     /locus_tag="PK198_19260"
     CDS             2723..3406
                     /locus_tag="PK198_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40942.1"
                     /translation="MEQQAFYIVFAIIGMAIVGIVSWAATYSSRNDYDDYYRHPGGYP
                     PPYYPPYPPHYPQPQRESPIMTLLGLGLVVFLIFSMMQYCERVEERKRLQRIHDEDNN
                     VVGVPPTVDSSNWGDSTNYEPPLTVPVTEYDVQPENERPPVSPHDGFYFQKSASHDDG
                     RILDAAQQLETSFPGRVFIGVVDDGTGFPYKLLIGPYDTEAAAQSVHGRGSNIYRPAD
                     EGVQIYNPR"
     gene            complement(3456..>3974)
                     /locus_tag="PK198_19265"
     CDS             complement(3456..>3974)
                     /locus_tag="PK198_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40943.1"
                     /translation="TTLANTLAVQDRALRKLLIETAMIGGNALSLTQVMTTPGRAGNE
                     FVPGQTAYSLQTGMAYTTQAPDEMAFRALIGDKTTFQVKYRYWMGRNSFDLSSSAASG
                     EFTLRIIEPNKNNKLWVRGVFSKFKTATGDFVSLDEVALFRIVAYNDEEFTLYFQSES
                     GVKHNIVLKIKD"
     assembly_gap    3975..3984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3985..4339)
                     /locus_tag="PK198_19270"
     CDS             complement(<3985..4339)
                     /locus_tag="PK198_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40944.1"
                     /translation="MKKSTLFTSLFLSMFFATVQAQTDSIFTRSEVIPCTVKEIGEQT
                     VSYSYPGEDVINKMYKNTIAKIVFRNGRVQTFEEFKALQPVLHAGDHAKVQILTTEVE
                     TKGLIKLDPVVSKAKG"
     gene            complement(4782..5171)
                     /locus_tag="PK198_19275"
     CDS             complement(4782..5171)
                     /locus_tag="PK198_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRF40945.1"
                     /translation="MQRIPSLYFADSQLGGRGVFSAAPIEEGSLIEICPVVICPAEDL
                     PIIHSTRLHDYYFLWGEEQQECAIALGFGSLYNHSFDPNAMYMPDFEGGVISFYSLRD
                     IPAGEEITVNYNGDPEDDNEVWFSVGE"
     gene            5253..>6042
                     /locus_tag="PK198_19280"
     CDS             5253..>6042
                     /locus_tag="PK198_19280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF40946.1"
                     /translation="MNTTGLTFGPIPSRRLGQSLGINNTLLPKACSYNCAYCQAGRSH
                     YLEIERRAFFSPQEIYKAVSKAIQNAEKEGVEINYLSFVPDGEPTLDIQLGESIRLLK
                     TLGKRIAVFTNASLIWRPEVRADLALADWVSLKFDAVLETAWRRTDRPQKYLSLPLIM
                     DGALAFARQFRGALVTETMLCADLNDMPENLTATAEYIATLSPSVAYLGVPTRPPLEK
                     WALPPDEAILTQAFAIFAARIPKVELMVGYPPPAYQPGNDAERDI"
BASE COUNT         1471 a         1653 c         1473 g         1435 t
ORIGIN      
        1 gagtgtaggt ttcgggttcc ctcgcgctgc aaaccgccta ccgccaactg atttcgggtt
       61 tcgtaccttt gccacatgga tcaccaaacg atgtattatc aaaaacgagc cggcgagtac
      121 gagcagattt accaaaagcc tgaaagacag gccgatctgc aacagttaaa ggcatacttg
      181 agccgccaat ggatggggaa acatattgtg gaaataggtt gcggcacagg gtattggacg
      241 gaaatactgg cccggcattg cgccttggtg acggcaacag acatccatca ggaagtcatc
      301 gaacaagcca aattgaaagc atacccccgg caaaatgtac agtttgagat catggatttt
      361 tatcaccctg agagacggca gccacggttg gaggggcttt ttgggggatt cgtatggtcg
      421 catgttccgt tgcaaggtct ccgcgatttt ttactccttt gtttagatca gatagaagca
      481 ggcgctgagc ttatttttat ggataatcaa tatgttgaag gaagcagcag ccccattcat
      541 cgaacggatg aacacggaaa cacctgccaa ttgcgcacgc tgaagtcggg agaaactttt
      601 gaaatcatca aaaatttccc cgataaaata tctgtccaac aatccatttc agacttatgt
      661 acagattttg aatggcttga attgaaatat ttctgggcgg caaaatttaa aaaaccggcg
      721 taaggcgcgc actaacagtg tattatacgc gatgaaaccc ccgcaatccc tccatcgggg
      781 ccgccagaaa tgtacccgct tgaagtccgg cctccgacaa aaccccttcc aaaaccccgc
      841 gaaaataaaa tgccaccgac cccgtaaaat gcaccggtaa tcgccgggca tcgggatagg
      901 gcagtacgca acgccgaacg aaaagatgaa actgccgctg caataactcc tgtacgaatg
      961 cactcaccaa atgcgccgag agaaagggtg taaaaccagc caaaaaccgg ctcggtgttt
     1021 cgccccgata tacccgctcg atcaccgcat ctctcgaaag cccgtattcc gcatcgaagg
     1081 cttgttctaa ttcagcgggc agttcccggt tgagccaggc gatgatgagt tccctgccca
     1141 gcacggcacc gctgccctcg tcgccgagaa tgtagccata gccgccgctg ttttgcaaca
     1201 cctgcgcgcc atcgtaccaa accgaattgg accccgtgcc gagaatgcag gcaatgccgg
     1261 cctgccgacc acacaaagcc cgcgctgccc ccagcacatc cgtctctatt tcgatggcgg
     1321 cggcagaggg aaaggctgcg gaaaatgctg cgtgcaggcg ctttctggag ctttctgcct
     1381 tacaaccggt gccgtaaaaa aagatgccgt caacggtgtc gccggtttga ccggcaatga
     1441 ccgccaattg cgcactgatt tcctcatccg acatgaaaaa gggaggaatt ccaggcgttt
     1501 tgaactcaga taccagttct ccggcctgca tcagcgacca atcggctttg gtagcgccgc
     1561 tgtcaatgat gagcacgcgg ttcatacctg aatggacgat tcgacgccgg aacgcgattc
     1621 gagcgctttg cgaacgctgc catgctccag cagcagggcc tcggcggtgg gatagtccaa
     1681 ttgcagggca ttcatcagga ttttggcgcc ccgatccacc aatttggtgt tgctcaattg
     1741 catgtccacc attttgttgt cgagcacgcg gcccatgcga atcatgaggg tggtggaaat
     1801 catgttgagc accaattttt gcgcggtgcc ggccttcatg cgggtgcttc cggtgacgaa
     1861 ctccggcccg gtgaccacct cgatgggaaa atcggcggct gcggccaaag gcgtgcctgc
     1921 attggaggcg atacagcccg tgaggatgcc gttggcccga cagtcgtgaa gaccgcctat
     1981 cacatacggc gtagtgccgc tggccgcgat gcccaacacc acatcttgtt gattgataaa
     2041 gtgttgcttg aggtctttcc atgcctgggt gggatcgtcc tctgcaaatt cgatgggttt
     2101 tcgtatcgcc gtgtctccgc cggcgatgat gccgatgacc cagttgtcgg cgacgccata
     2161 agtgggcggt atttccgaag cgtcgaggat gcccaatctg ccgctggtgc cggcgccaat
     2221 gtaaaagaga cgaccgccgt tgttcatttt ttctaccaat gcctccacaa atttttcaat
     2281 ttgcggcagc gctttttcta ctgctaaggg cacggtttgg tcttcctgat tgataccttt
     2341 caacagttcg gcaaccggca tttgctccaa atgcctgtaa tgagagggtg cttctgtgag
     2401 tttttgcatg atctgacagt attggctgcg ccaaagtagc aagttccggc aatttgtaca
     2461 ctatactgtg ataaaaaaca gcaaaaagca gcaggctcgc acgaaaaaag cgggacgttc
     2521 cgaaaaaata attccttttt tttcgtgtga aggaactatc aattctgcta ttcatttatt
     2581 tgtattaaac caactactcg ttccaaaaca ctatatttca atgaattacc gaggaggtaa
     2641 ggaaccggag ggcacagctc ttcagggatg gcatggcttt ggcaataagt tttgagttac
     2701 ataccattaa atatagatca acatggaaca gcaggctttc tacatcgtgt ttgccattat
     2761 cggcatggcc attgtcggaa tcgtttcctg ggcggcaacc tactcttcca gaaatgatta
     2821 cgacgactac taccgccatc cgggaggcta cccgccgccc tactacccgc cgtatccgcc
     2881 gcactatccg cagccgcaac gggaatctcc catcatgacc ttactgggct tgggcctggt
     2941 ggtatttctg atcttctcga tgatgcaata ctgcgagaga gtggaggagc gcaagcgttt
     3001 acagcgcatc catgacgaag ataacaacgt cgtgggggtg ccacccactg ttgattcttc
     3061 caactggggt gattcaacaa attatgagcc tccgcttacg gtacctgtaa ctgaatacga
     3121 tgttcagccc gaaaacgaac gtcctcctgt ttcgccccac gacggcttct atttccaaaa
     3181 gtccgcctcc catgacgatg gtcggatttt ggacgccgcg cagcaattgg aaacaagctt
     3241 tccgggcagg gttttcatcg gcgttgttga cgacggcacc ggatttccct acaaattgct
     3301 gatcgggcct tacgacaccg aggcggctgc ccaatccgta cacggtcggg gcagcaacat
     3361 ctaccgtccg gcagacgagg gcgttcagat ttacaatccg cgatgacatt ccgtacgttt
     3421 gctaagctcc tccggggttt cgtaaacgta cgtctttaat cctttatttt cagcacaata
     3481 ttgtgcttta cgcccgattc gctctgaaag tacaatgtga actcctcatc gttgtaagct
     3541 actatgcgaa acaaagcaac ctcgtcaagc gaaacaaaat ctcctgtggc tgttttgaat
     3601 ttggagaaca cgccccgcac ccaaagtttg ttattcttgt tgggttctat gattctcaac
     3661 gtaaattctc ctgaagcagc agaagaactg agatcaaaac tgtttcttcc catccagtac
     3721 ctgtatttta cctgaaaagt ggttttatcg ccgatcagcg cccggaaagc catctcgtcc
     3781 ggagcttgag tggtataggc catgccggtt tgcaggctgt aggccgtctg gcccggaaca
     3841 aattcgttgc cggcacgtcc cggagtagtc attacctgcg tgagggataa ggcattcccg
     3901 ccgatcatgg ccgtttcgat caatagtttg cggagtgccc ggtcttgcac tgccagcgta
     3961 ttggccagcg tagtnnnnnn nnnncgcctt ttgccttgct gactacgggg tccaatttta
     4021 tcaatccttt cgtttcaacc tctgtggtca aaatctgcac tttggcatgg tctcctgcat
     4081 gcaaaacggg ttgcaaggct ttaaattcct caaaagtctg tacccgcccg tttcggaata
     4141 cgattttggc aatggtgttt ttgtacatct tgttgatcac gtcctcgccg gggtaagaat
     4201 agctgacggt ttgttcgcct atttccttca cggtgcaggg gatcacttcc gaacgcgtga
     4261 aaatactgtc tgtttgcgct tgaacagtgg cgaaaaacat agacagaaag aggctggtaa
     4321 agagcgtaga ttttttcatg tagagattgt ttttgtggtg aataggaaag gaatgataaa
     4381 caatcaacaa cattacagcg ctgctgtttg gcctgccgct gcggagttgc gttaatcttt
     4441 tctaaaaaga tggtttcagg ttcagcgttc cgggttccgg gttccgagtt tcacgttccc
     4501 tcgcgctgtg ccacgacgga ggcgtggttc gcggaaattc gcgtatttcg cattcattcg
     4561 cgttaaaact tcgcgttccg cgttccggtt tttcgacgct gaaagacgca ggtttttatg
     4621 atcgttatgc ccctctccga gcagcggaaa tcataaaaat cataaagatc agcgtcaaaa
     4681 acaacgcgcg ggttccgggg gtagtgaagg ggtgactcag caagtttcag ccccgcagtc
     4741 accccttcac taagctggag actcatccac tcactcctca ctcactcacc cactgaaaac
     4801 cacacctcat tatcatcctc cgggtcgccg ttgtaattca cagtaatctc ctcccccgca
     4861 ggtatatccc tcagactgta aaaactaatg acacccccct caaaatcagg catatacatc
     4921 gcattgggat caaacgaatg attgtacaaa gacccaaacc ccagcgcaat ggcacactcc
     4981 tgctgctcct cgccccagag aaaataatag tcgtgcaacc gggtgctgtg aataatcggc
     5041 aggtcttcag ccggacatat cacaaccggg caaatttcaa tcagcgaacc ctcctcaatg
     5101 ggcgcagccg aaaaaacacc acgacctccc agctggctgt cggcaaaata aagcgaagga
     5161 atgcgttgca tacaggcaaa tgacttttgt cagccaaaga aacgcaacaa tgcacataac
     5221 ttgcctcgga tttatagaaa aaaatcacca atatgaacac taccggtctc accttcggcc
     5281 ccattccctc gcgcaggctg ggccagagcc tcggcatcaa caacaccctg ctgcccaagg
     5341 cttgttctta caattgcgcc tactgtcagg ctgggcgctc ccattatttg gagatagaaa
     5401 ggagggcttt tttttcgccg caggagattt ataaagctgt ttcaaaggcc atccaaaatg
     5461 ctgaaaaaga aggtgttgaa atcaattacc tcagcttcgt acccgatggc gaacctacgc
     5521 tcgatatcca gcttggcgag tccatcaggc tgctcaaaac cctgggcaaa cgcatcgccg
     5581 tttttaccaa cgcctccctg atctggcggc ccgaagtgcg cgccgatctg gccctcgccg
     5641 actgggtatc gctcaagttc gatgccgtgc tcgaaaccgc ctggcgccgc accgaccgac
     5701 cccagaaata cctcagcctg cccctcatta tggacggagc gctcgccttt gcccgccaat
     5761 tccggggcgc attggtcacc gaaaccatgc tctgcgccga cctcaatgac atgcctgaaa
     5821 atctcaccgc caccgctgaa tacatcgcaa cactctctcc ctcagtggca tatctcggag
     5881 ttcccacccg cccgcctttg gaaaaatggg ctttgccgcc cgatgaggcc attcttaccc
     5941 aggcttttgc catatttgca gcgcgcattc caaaagtgga actcatggtg ggctacccgc
     6001 ctccggccta tcaaccgggc aacgatgccg aacgcgacat tc
//
LOCUS       DAORLF010000295         6029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7129_length_6029_cov_2.011048, whole genome shotgun sequence.
ACCESSION   DAORLF010000295 DAORLF010000000
VERSION     DAORLF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_6029_cov_2.011048"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK198_19285"
     CDS             <1..231
                     /locus_tag="PK198_19285"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HRF40947.1"
                     /translation="SKFSGEMVYVGDWHSHPNGTNQYSQDDYHSIKSVAISSNVNTHN
                     PILMIAAFGKDYFSPGFYVFHDDKLLMYELVQ"
     gene            301..>2660
                     /locus_tag="PK198_19290"
     CDS             301..>2660
                     /locus_tag="PK198_19290"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRF40948.1"
                     /translation="MNSSVSPHQAKYFAYELTKRCPSDSVEKFSATLLDAQVDLNPHQ
                     VEAALFAFKSPLSTGAILADEVGLGKTIEAGILLSQKWAENNRKLIVICPSNLRKQWS
                     QELEEKFFLPTQILELRSFNEFIKAGKRNPFEQPKVLICSYHFSHSKEAFLQLVSWDL
                     VVIDEAHRLRNVHQPSNIIGKSVKNATAHARKVLLTATPLQNSLMELFGLVSIIDEHV
                     FGDRMSFKNQFTRPGGEVDFQELRNRIAPVCHRTLRRQVQEYIRYTNRIPLTLRFEPT
                     EAEYDLYVKLNEYLQRQLLFALPAGQRHLLTMIMRKLLASSSFAIAGTLHKLISRLEL
                     LAQQGEARLESLEGFDEDLDDLDDYLDEWEYADEEESISLSAEEIEQVKTEIEELKHY
                     RDLAESIESNAKGEKLIASLREGLSKMEALKAPQKAIIFTESRRTQAYLFNLLEQTDF
                     QGATVLFNGVNNDDKSKEIYREWLAKNRNTDRSTGSHSADMRAALVEYFRDEARIMIA
                     TEAAAEGINLQFCAMVVNYDLPWNPQRIEQRIGRCHRYGQRHDVVVVNFLNLKNAADQ
                     RVFQLLDEKFQLFSGVFGASDEVLGTIESGVDFEKKIAAIYQHCRSTEEINRSFDLLQ
                     AELEEQINNRIKNAREQLFEHFDAEVIDKLKVRLEDTKSYIGKYEHWLWRIALYALQD
                     FAEFDPTRFMFRLYALPQGAEAPIGWYTLDKKQDAQHRLRIGHPLAQAIIEQAKTSLT
                     PTARITFNYSGTRSTSEMLEPLLGQSGYLMLSSLTVHSFDTTDHLV"
     assembly_gap    2661..2675
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2676..>3122
                     /locus_tag="PK198_19295"
     CDS             <2676..>3122
                     /locus_tag="PK198_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40949.1"
                     /translation="DLRQQRRARQLEQCRRLMGLDALGWETPETDVATLRRLADEHTS
                     DQKTLLQELKDKDTVWFRTETLKLQKWADDRIYAAEKAIRDTKAKIRELERLSAQETN
                     TETLLDAQKKISELKKKQRRQRTEIFDVEDEIEAQRDAMIREVEVR"
     assembly_gap    3123..3146
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3207..>3504
                     /locus_tag="PK198_19300"
     CDS             3207..>3504
                     /locus_tag="PK198_19300"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HRF40950.1"
                     /translation="MDFIALLEKLRQTHDLIQQRVATAISNGLVVRNWLIGHYIVEFE
                     QNGRDYAQYGERLLYRLSEELAQGGMKGMSYTNLTLYRKFYLGYPHLSSIAGRFS"
     assembly_gap    3505..3530
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3538..>4044
                     /locus_tag="PK198_19305"
     CDS             3538..>4044
                     /locus_tag="PK198_19305"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
                     /protein_id="HRF40951.1"
                     /translation="MAEDLSDWQLPAETLLKRLSFTHFVELLKLDNPMQRLFYEMECI
                     KGNWSRRQLQRQIGSLLFERTGLSRNKEALLAQIKDDNSPIHIADFIREPYVFEFLGL
                     KMEDAFLESDLEKALLEHLQSFLLELGNGFCFEARQKSFLIDNRRYKIDLLFYHRILK
                     CHVVVDLKT"
     assembly_gap    4045..4066
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4067..4312
                     /locus_tag="PK198_19310"
     CDS             <4067..4312
                     /locus_tag="PK198_19310"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRF40952.1"
                     /translation="PEDAGQMNFYLNFHRENLMTEDDNPPVGIILCTYKNEAVVRYAT
                     GSLDNQLFISRYLLQLPNEEILRNFLLQEKRLLGDIQ"
     gene            4365..>4550
                     /locus_tag="PK198_19315"
     CDS             4365..>4550
                     /locus_tag="PK198_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40953.1"
                     /translation="MLSYLADLSKTGCAKGDDLRSSLLYLFLFQPVLRMTNYDQLIKV
                     LKELFQLNQAELDFGIYR"
     assembly_gap    4551..4600
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4601..5989
                     /locus_tag="PK198_19320"
     CDS             <4601..5989
                     /locus_tag="PK198_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40954.1"
                     /translation="QLIPQVQAIIAQSNLGDKSALETELAEKIAQARQLGVADPDSLD
                     TVKLLRNEIARTPDPAALENEIFSHLAAFFKRYYKEGDFISMRRYKKDVYAIPYEGEE
                     VKLYWANHDQYYIKTAEHLKNYAFKLPHDRLVRFELAEANTEKDNRKEESGKERRFRI
                     YAQKPVETDGNTLRVFFSYEPSDRKEKQEDLNAAALALVKKHLPADWAADVLQKAPTD
                     KNKDRSLLEKHISDFTARNTFDYFIHKDLGGFLSRELDFYIKNEVLHLDDVQPDEPAE
                     LRRQLVKIRALRDVGGKIIAFLAQLENFQKKLWLKKKFVTDTQYCITLDRIPEAFYPE
                     IAANDAQRQEWVRLFAIDEIQPDLVQPPYSVPLSVGFLQSQPFLVLDTAFFSEDFKLR
                     LIASFDDLDEQLDGLLINSENFQALNLLQERYKGQAKCVYIDPPYNTNSAPILYKNDY
                     KYSSWASLINGS"
BASE COUNT         1608 a         1710 c         1317 g         1257 t
ORIGIN      
        1 agtaaatttt caggagaaat ggtttacgtt ggggattggc atagccatcc taatggcacc
       61 aatcaataca gtcaggatga ttatcattcc atcaagagcg ttgccatctc ctcgaacgtc
      121 aatacacata acccgatttt gatgattgct gccttcggta aggattattt ctctcctggg
      181 ttttatgtgt ttcatgatga taaactgttg atgtacgagc tggtacaata aaaatcacat
      241 agactttctt cgtgctctcc ccaaaaaaca gttcttttgc atgtcaactt acaagggtct
      301 atgaattcat cggtatctcc ccatcaggcc aagtacttcg cttatgaact caccaagcgc
      361 tgtccttctg atagcgtaga gaagttcagc gccactctgc tggatgctca ggtggacctg
      421 aaccctcatc aggtagaggc cgcgttgttc gctttcaaat ctccgctttc caccggggcc
      481 atactggctg atgaagtggg ccttggcaaa accattgaag ccggcatact cctttctcaa
      541 aaatgggcag aaaacaaccg caagctgatc gtcatctgcc cctccaatct gcgcaagcaa
      601 tggagccagg aactggagga aaagtttttt ctacccaccc aaatactgga acttcgcagc
      661 ttcaacgagt tcataaaggc aggcaaacgg aaccccttcg agcagcccaa agtgctgata
      721 tgttcttatc acttctcgca cagcaaagag gcctttctcc aattagtaag ctgggacttg
      781 gttgtcattg atgaagcgca ccgcctgcgc aacgtgcatc agccgtccaa catcatcggc
      841 aagtcggtaa aaaatgcgac ggcacatgcg cgcaaggtat tgctgacagc tacgcccctg
      901 caaaattccc tgatggagct tttcggcttg gtcagtatca tagacgagca tgtattcgga
      961 gacaggatga gctttaaaaa ccaattcacc cgacccggtg gggaggtaga ttttcaagaa
     1021 ctccgaaacc gcatcgcccc ggtatgtcac cgcaccttgc gcagacaggt acaagaatac
     1081 attcgctaca ccaaccgcat tccgctcacc ctgcgttttg agcctaccga ggccgaatac
     1141 gacctctatg tcaaactgaa cgaatacctc caacggcaac ttctcttcgc cctgcccgca
     1201 ggccagcgcc acctgctgac tatgattatg cgcaaactgc tcgcctcatc gagctttgcc
     1261 attgcaggca ccctgcataa gctcatttca aggttggaac tcttggcaca gcagggagaa
     1321 gccagattgg aatcgctcga aggttttgac gaggacctgg acgatctcga tgactacttg
     1381 gacgaatggg aatacgccga tgaggaggaa agtatttctc ttagtgcaga ggaaattgaa
     1441 caggtcaaaa ctgaaatcga agagctgaaa cactaccgcg acctggccga gtccattgag
     1501 tccaatgcta aaggagaaaa actgatcgca tcattgcgcg aagggctatc caaaatggaa
     1561 gcgcttaagg ctcctcaaaa agctattatt tttacagaaa gccgccgcac tcaggcatac
     1621 ctttttaatt tgctggaaca gacagatttt cagggtgcta ctgtcttgtt taatggagtg
     1681 aacaacgacg acaagtccaa agagatatac cgggaatggt tggcgaaaaa ccgcaacacc
     1741 gaccgcagca ccggttcgca cagtgcagat atgcgggcag cgttggtgga gtatttccgc
     1801 gatgaagccc gcatcatgat cgccaccgaa gcggcagcgg agggcatcaa ccttcagttt
     1861 tgcgctatgg tggtcaatta cgacctgccc tggaaccccc aacgcataga gcagcgcatt
     1921 ggccgctgcc accgctatgg acagcggcat gacgtagtgg tggtcaactt cctcaacctg
     1981 aaaaatgctg ccgaccagcg ggtcttccaa ctgctcgatg aaaaatttca gcttttcagc
     2041 ggggtcttcg gcgcttccga tgaggtcttg ggcaccattg aaagtggcgt tgattttgaa
     2101 aagaaaatag ccgccattta ccagcactgc cgaagcaccg aagaaatcaa ccggtctttt
     2161 gacttgttac aggctgaatt ggaggaacaa atcaacaacc gcatcaaaaa tgcacgggaa
     2221 caactgtttg aacactttga tgccgaggtt attgataaac tcaaagttcg tttagaagat
     2281 accaaaagct acatcggcaa atacgagcac tggctctggc gcattgcctt gtatgctttg
     2341 caggacttcg ccgaattcga cccaacgcga ttcatgtttc gcctctatgc tttgcctcaa
     2401 ggcgcagagg cgcctatcgg ttggtacact ttggacaaaa agcaggatgc ccagcaccgg
     2461 ctgcgcatag gccatccgct ggcccaggcg atcatcgagc aggctaaaac gtcgctcacc
     2521 ccaactgccc gcattacttt taactattcc ggcacccgca gcacctccga aatgctggaa
     2581 ccactgctcg gccaaagcgg ttacctgatg ctaagcagtc tcaccgtaca ttccttcgac
     2641 actaccgacc acctcgtttt nnnnnnnnnn nnnnntgatc tgcggcagca gcggcgcgct
     2701 cgccagcttg agcaatgccg ccgcctgatg ggtttggatg ccctcggctg ggaaacgcct
     2761 gaaacagatg tagcaaccct gcgccgcctg gccgacgagc atacctccga tcaaaagacc
     2821 ctgcttcagg aactgaaaga caaagacacc gtatggttca ggaccgaaac gctcaaactc
     2881 cagaaatggg ccgatgaccg catttacgcc gccgaaaagg ccatacgcga caccaaagcc
     2941 aaaatacgcg aactggaacg cttgtccgcg caggaaacca acacagaaac cctcctcgac
     3001 gcgcaaaaaa agatcagcga actgaagaaa aaacaacgcc gccaacgcac cgaaatcttt
     3061 gacgtggaag acgaaattga agcccaaaga gacgccatga tacgggaagt ggaagtgaga
     3121 atnnnnnnnn nnnnnnnnnn nnnnnnccag actcctttat ttgccattca atggctactt
     3181 ttgtaatctt ctaattttcg ccaatcatgg actttatagc cctgcttgaa aagctccgcc
     3241 aaacccacga cctcatccaa cagcgggtcg caactgccat cagcaacgga ctggtcgtcc
     3301 gcaactggtt gataggccat tacatcgtag agtttgaaca aaacggccgc gattatgcac
     3361 agtatggcga acgcctgctc tatcgccttt ccgaagaact cgcccaaggc ggtatgaaag
     3421 gcatgtctta taccaacctc accttgtatc ggaaatttta cctcggatat cctcacttgt
     3481 cgagtatcgc aggcaggttt agcannnnnn nnnnnnnnnn nnnnnnnnnn tcagccagtg
     3541 gctgaagatt tgagcgactg gcaactgccg gctgaaacat tgttgaaaag gttgtccttc
     3601 acgcatttcg tggagttatt gaagctagac aaccccatgc aacggctctt ttacgaaatg
     3661 gagtgcatca agggcaattg gtcacggcgc cagctccaac gccaaatcgg cagcctcctc
     3721 ttcgagcgca ccggcctgtc gcgcaacaaa gaagccctct tggcccaaat aaaagacgac
     3781 aacagcccga tccatatcgc cgacttcatc cgggagccgt atgtgttcga gttcctcgga
     3841 ctgaaaatgg aagatgcgtt tttagaaagc gatttggaaa aagccctcct cgaacacctg
     3901 caatccttcc tgctcgaact cggcaacggc ttctgcttcg aggctcgtca aaaaagcttt
     3961 ctcattgata accgccgcta taaaatcgac ctgctctttt accaccgcat cctcaaatgc
     4021 catgtggtgg tggatttgaa aaccnnnnnn nnnnnnnnnn nnnnnncccg aagacgccgg
     4081 ccaaatgaat ttctacctca atttccaccg agaaaatctg atgactgaag acgacaaccc
     4141 gccggtaggc atcattctct gcacctacaa aaacgaggcg gtcgtgcgct acgccacagg
     4201 ctcactcgac aatcaacttt tcatctcccg ctacctgctg caactcccca acgaggaaat
     4261 cctccgtaat ttcctcctgc aagaaaagcg gctcctcggc gatattcaat aaatattcct
     4321 cctgccacac tacaaaacca tccaatgtcc tcatttactt cttcttgctt tcctatcttg
     4381 ccgatctttc aaagaccggt tgcgccaaag gcgatgatct gcgaagcagc ttgctatact
     4441 tgttcctttt tcaacccgta ctccgcatga ccaactacga ccaactcatc aaagtcctca
     4501 aagaactctt ccagctcaat caagccgagc tggacttcgg tatttaccgc nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caactcatac cacaggtaca
     4621 ggccatcatc gcccagagca acctcggcga caagtcggca ctcgaaaccg aactggccga
     4681 aaaaatcgcg caggcccgcc aattgggcgt agcggacccc gattccttag acaccgtcaa
     4741 gctcctccgc aacgaaatag cccgcacacc cgatcctgcc gccctcgaaa acgaaatttt
     4801 ctcccacctc gccgccttct tcaaacgata ctacaaagaa ggcgacttca tctccatgcg
     4861 ccgctacaaa aaggacgtgt acgccattcc ctacgaaggc gaagaagtaa aactctactg
     4921 ggccaatcac gatcagtact acatcaaaac cgcggaacac ctcaaaaact acgccttcaa
     4981 actgccacat gaccgcctcg tacgcttcga attggccgaa gccaacaccg aaaaagacaa
     5041 ccgcaaggag gaaagcggca aagagcgccg attccgcatc tatgcccaaa agcccgtgga
     5101 aacagacggc aataccctcc gcgtgttctt cagctacgaa ccttccgaca gaaaggaaaa
     5161 acaggaagac ctcaacgccg ccgcgctcgc ccttgtgaaa aaacacctgc ccgccgactg
     5221 ggccgccgac gtgctgcaaa aagcgcccac cgacaaaaac aaagaccgca gcctgctgga
     5281 aaaacacatc agcgacttca ctgcgcgcaa tactttcgac tacttcatac acaaagacct
     5341 cggcggcttc ctgagccgcg aactcgattt ctacatcaaa aacgaggtgc tgcacttgga
     5401 cgacgtacag cccgacgaac cagccgaact gcgccgccaa ctcgtgaaaa tacgcgccct
     5461 gcgagatgta ggcggcaaga tcatcgcctt cttagcccaa ctcgaaaatt tccagaaaaa
     5521 gctctggctc aaaaagaaat tcgtaaccga cacccagtat tgcatcaccc tcgaccgcat
     5581 accggaggcc ttctaccccg aaatagccgc caacgatgcc caacgccagg aatgggtgcg
     5641 cctctttgcc attgacgaaa tacagcccga cctcgtgcag ccgccctact ccgtgccgct
     5701 cagcgtgggc ttcctccaaa gccagccctt cctcgtactc gataccgcct tcttctccga
     5761 agacttcaag ctgcgcctca tcgcatcctt cgacgacctc gacgaacaat tggacggcct
     5821 gctcatcaac tccgaaaact tccaggcact gaacctgctg caggagcgct acaaaggaca
     5881 ggcgaaatgc gtgtatattg atccgccgta taatacaaac tcagctccaa ttctgtataa
     5941 aaacgactat aagtactctt cttgggcctc tttaatcaat ggttcgtgaa gaatcgccgc
     6001 tttgcggcgt gggcttgtga gaaataaaa
//
LOCUS       DAORLF010000296         6013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7155_length_6013_cov_1.791541, whole genome shotgun sequence.
ACCESSION   DAORLF010000296 DAORLF010000000
VERSION     DAORLF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_6013_cov_1.791541"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>811)
                     /locus_tag="PK198_19325"
     CDS             complement(81..>811)
                     /locus_tag="PK198_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF40955.1"
                     /translation="PANPTLACVDIEALAAIARRHGRHSAIDNTFCTPYLQQPLALGA
                     DFAIHSTTKYLNGHGNSIAGAIVGRDAALMRDKVAKTMKLLGANCNAWDAWLTNLGMK
                     TLTLRMDRHCANALALAQFLQQQPAVAKVNYPGLESHPDHALARRQMRNFGGMLSFEL
                     TAGYDAAIACMNQLTFCSLAPTLGDVDTLILHPASSSHLNIPKELRLQNGITDGLVRV
                     SVGIEDLSDLIADFQKGLAAAIAK"
     assembly_gap    812..838
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<839..1284)
                     /locus_tag="PK198_19330"
     CDS             complement(<839..1284)
                     /locus_tag="PK198_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRF40956.1"
                     /translation="MSAISFNSLCVHDPEDPRSTRPHQLPIYATSSFDFESIDQGIDI
                     FSGKAQGHVYGRYGNPTIDAVAGKIAQLETHGLDIEASAILCSSGMAAIAVLMTSLLR
                     SGDKILTQGNLYGGTTELFTKTLAQLGIQTVMTDLQKLEQVEALVRA"
     gene            complement(1420..>1622)
                     /locus_tag="PK198_19335"
     CDS             complement(1420..>1622)
                     /locus_tag="PK198_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40957.1"
                     /translation="IACETSLGHAADYDDTAAIRTAILSMYQDFISRKSALRAPLPDA
                     SHPYSIVRLTGQIAALLDEISR"
     assembly_gap    1623..1632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1633..>1995)
                     /locus_tag="PK198_19340"
     CDS             complement(<1633..>1995)
                     /locus_tag="PK198_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40958.1"
                     /translation="DKFSITHLGILGYDRNPPALFQVLQELCISQPGFRQHLEIRLVG
                     QLDPTVLDAIQAAKLEDRTLMPGNVSRQEALQFTSSSEVLLLLLNRQSNAMGRIPGKL
                     FEYIAVARPILVLGSPASD"
     assembly_gap    1996..2036
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2037..2340)
                     /locus_tag="PK198_19345"
     CDS             complement(<2037..2340)
                     /locus_tag="PK198_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF40959.1"
                     /translation="MTKYLKENPVDAIFSDGPPHSNTRIATLLKKATGIPWLADFQDP
                     WTQVDYYQLLHLTHWGDRRHRRMEQEAFRMADRMTIVSPTWKNDLEQIGARNVSVIP"
     assembly_gap    2368..2405
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2406..2735)
                     /locus_tag="PK198_19350"
     CDS             complement(<2406..2735)
                     /locus_tag="PK198_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40960.1"
                     /translation="MAGKKVLIISYYWPPSGGIGVLRCLKIAKYLRQFGWEPVIFTAD
                     NAHYPSIDHSNDHDIPPGALILRQRIWEPYHLYKWFTGKPKTANVNNVFYVQENRSDW
                     KHYLSVWL"
     gene            complement(2735..3373)
                     /locus_tag="PK198_19355"
     CDS             complement(2735..3373)
                     /locus_tag="PK198_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF40961.1"
                     /translation="MRIETNGMGKRYRREWILRQADLQLLPGHAYAITGPNGAGKSTL
                     LRLLSGHLTPSKGSIRFFDAQSEIPTAEAYRHISYAAPYIELIEELTLRESLQFHRQF
                     RPFLNGMTDKDIIELLAFRKAEDKQIRHFSSGMKQRLKLALALCSDASAVLLDEPTTN
                     LDRQGMDWYHNLVKTMRGDRLLIVASNVEEDLQFCEERIDILAFKNPATPAR"
     gene            complement(3363..4181)
                     /gene="lpxA"
                     /locus_tag="PK198_19360"
     CDS             complement(3363..4181)
                     /gene="lpxA"
                     /locus_tag="PK198_19360"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRF40962.1"
                     /translation="MNKQATQSYIHPEAQIGENVEIGPFCYIDRDVVIGDNTWIGPHV
                     TIFDGARIGSHCRIFPGAVISAIPQDLKFNGEETTAEIGSHTTIREYVTVNRGTVAAG
                     RTIVGSHCLLMAYVHVAHDCILGNHVILANNVNLAGHVEIDDFAILEGLVAVQQFVRI
                     GRHSFIAGGSLVRTHVPPYIRAAREPLAYVGINKVGLSRRQFPEELIGHITDIYRLLF
                     VRGYNTSHALGEIETLLPLTEQRQEIVDFVQQARRNGGLVKGVQKLNGVKVHED"
     gene            complement(4174..>4835)
                     /gene="fabZ"
                     /locus_tag="PK198_19365"
     CDS             complement(4174..>4835)
                     /gene="fabZ"
                     /locus_tag="PK198_19365"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRF40963.1"
                     /translation="TLDLQFKNEPARHKLLDVIGDIALLGKPIKGKITAIKPGHRANV
                     EFTRLLRKAMLEQRKLKGKPKYDPDKEALFNTQQIAEWLPHRYPMLLVDKIIELSDTH
                     VVGVKNVTFNEQFFLGHFPNNPVMPGVLQIEAMAQTGGILAMSIMGDPGQWDTYFLKI
                     DNAKFKSKVVPGDTLLLKLELMAPIRRGICQMLGTAYVGNKIVSEAELTAQIVKRSNN
                     E"
     assembly_gap    4836..4967
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4968..>5283)
                     /locus_tag="PK198_19370"
     CDS             complement(<4968..>5283)
                     /locus_tag="PK198_19370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRF40964.1"
                     /translation="DGSARYFVEALREAGIEEQIEEREYFEITEPITYRDPVTGSELV
                     AMPSDCFEVTTLIDFNSPVLGQQFATISNLDNYAEEIAPCRTFVFLHEVEYLFSQNLI
                     KGG"
     assembly_gap    5284..5340
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5341..5624)
                     /locus_tag="PK198_19375"
     CDS             complement(<5341..5624)
                     /locus_tag="PK198_19375"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRF40965.1"
                     /translation="MPTVFTEALSKMKQRTIQRQVALSGVGLHTGRQVNLQFIPAGPN
                     HGYRFQRTDLPDQPVIPADVARVVSTQRGTTLQVDEAEVHTVEHALSALTG"
     assembly_gap    5723..5796
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5802..>6013)
                     /locus_tag="PK198_19380"
     CDS             complement(5802..>6013)
                     /locus_tag="PK198_19380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40966.1"
                     /translation="VDLTQYVGRDVRIEVYSIEGKLLQFREIKEVQYVIETMNLASFE
                     NGMYLVKVKSAGRLDKTKRVVLARE"
BASE COUNT         1189 a         1328 c         1764 g         1353 t
ORIGIN      
        1 cgacgcagga ggagcgaccg aggcacccga cggccccttt tgatggtatg agtgggccgg
       61 cacgccccga caaaaataca tcatttggcg atagccgcag cgagtccttt ttgaaaatcg
      121 gcgatgagat cggagaggtc ttcgatgccg acggagacgc gcacgaggcc gtcggtgatg
      181 ccgttttgga ggcgcaactc cttgggaatg ttgaggtgcg atgaagaggc cgggtgcagg
      241 atgagcgtgt ccacgtcgcc gagggtgggg gcgagggagc agaaggtaag ctggttcatg
      301 caggcgatgg cggcgtcgta tccggcggtg agttcgaagc tgagcatgcc gccgaagttg
      361 cgcatttggc ggcgggcgag ggcgtggtcg ggatgcgatt cgaggccggg atagttgact
      421 ttggcgacgg cgggctgctg ctggaggaac tgcgcgaggg cgagggcgtt ggcgcagtgg
      481 cggtccatgc ggagggtgag ggttttcatg ccgaggttgg tgagccaggc atcccaggcg
      541 ttgcaattgg cgccgaggag cttcatggtt ttggccactt tgtcgcgcat gagggcggcg
      601 tctcggccaa cgatggcgcc agcgatggag ttgccgtggc cgttgaggta tttggtggtg
      661 gagtggatgg cgaaatcggc gccgagggcg agtggctgct ggaggtaggg tgtgcagaag
      721 gtgttgtcaa tggcggagtg gcggccgtgg cggcgggcga tggcggcgag ggcttcgatg
      781 tccacgcagg cgagggtagg attggcgggg gnnnnnnnnn nnnnnnnnnn nnnnnnnngc
      841 gcgcacgagg gcctccacct gttcgagttt ttggaggtcg gtcatgacgg tttggatgcc
      901 gagttgggcc aatgtcttgg taaacagttc ggtagtaccg ccgtagaggt tgccctgggt
      961 gaggattttg tcgccggaac ggagcaggga ggtcatgagt acggcgatgg cggccatgcc
     1021 ggaactgcag aggatggcgg aggcttcgat gtcgaggccg tgcgtttcga gttgggcgat
     1081 tttgccggca acggcgtcga tggtggggtt gccgtagcga ccgtaaacgt ggccctgtgc
     1141 tttgccgctg aagatgtcta tgccctgatc tatggactcg aagtcgaagg aggaggtggc
     1201 gtagatgggc aactggtgcg gccgggtgct gcgcgggtcc tcgggatcgt gtacgcagag
     1261 ggagttgaag gagatggcgc tcatgatggt tgacgtattt ttgggtgcaa agaaaggaaa
     1321 aaggtttggg atggcgggtg gaacccgcag gatagatcac gcaaggtgga accttgcgcg
     1381 agcatggttt ttggggtgca aagaaaggaa aaaggtttgt caacggctga tctcgtcgag
     1441 caaggcggct atttgccccg tgaggcgaac gatggagtag gggtgggagg catcgggcag
     1501 cggtgcgcgc agggcgcttt ttcgggatat aaaatcctga tacatagaca gtattgcggt
     1561 tctgatggct gcggtgtcgt cgtagtcggc tgcatggccg aggctggttt cgcaggcaat
     1621 gcnnnnnnnn nngtcggatg cgggcgaacc gagtaccagt atggggcggg ctacggcgat
     1681 gtactcaaac aacttgcccg gtatgcggcc catggcgttg ctctggcgat tgagcagcag
     1741 cagcagcact tcgcttgatg aggtgaattg caaagcctcc tgtcggctca cgttgccggg
     1801 catgagggtg cgatcttcta attttgcggc ttgtatggca tcgagtacgg tgggatccaa
     1861 ttgtcctacc aacctgattt ccaagtgttg gcgaaagccg ggttggctga tgcaaagttc
     1921 ttgcaatacc tgaaacaagg ccggcggatt gcggtcgtag cccaaaatac cgaggtgcgt
     1981 gatgctgaat ttgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggg
     2041 aatgacgctg acatttcggg cgccgatttg ctccagatca tttttccagg tgggacttac
     2101 gatggtcatg cggtcggcca tgcgaaaggc ttcctgctcc atgcggcggt gccggcggtc
     2161 gccccaatgt gtgaggtgga gcagttggta atagtccacc tgcgtccagg ggtcttgaaa
     2221 atcggcgagc cagggaatgc cggtcgcttt tttgaggagg gtggcaatgc gggtattgga
     2281 gtgcggcggg ccgtcggaaa aaatggcgtc caccggattt tctttcaggt attttgtcaa
     2341 aaaacgcacg gagggccgaa tccaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnncagcc acacggagag gtagtgtttc cagtcggagc ggttttcctg cacataaaaa
     2461 acattgttta cattggcggt tttaggcttg ccggtgaacc atttgtagag gtggtacggc
     2521 tcccaaatcc gttgtcggag aatgagggcg ccgggcggaa tgtcgtggtc gttgctgtgg
     2581 tctatgctgg gatagtgggc gttatcggcg gtaaaaatga cgggttccca gccgaattgg
     2641 cgcaggtatt tggcaatttt gaggcatctc aacaccccga tgcctcccga cggcggccaa
     2701 tagtaactga tgatgagcac ttttttgccg gccattatcg agcgggtgta gcagggtttt
     2761 taaatgccag aatatcaatg cgttcttcgc aaaattgcaa atcctcttcc acgttggagg
     2821 ctacgatgag cagtcggtcg cctcgcatgg ttttgactaa attgtgatac cagtccattc
     2881 cctgccggtc gaggttggtc gtgggttcat ccaacaaaac ggcggaagca tcggaacaca
     2941 aagccagagc gagtttgagg cgttgtttca tgccggatga aaagtgccgg atttgtttgt
     3001 cttcggcttt tctgaaggcc agcagttcga tgatgtcttt gtctgtcatg ccgttgagaa
     3061 aaggccggaa ctgccggtga aactgcaaag actcgcgcag ggtgagttct tcgatcaatt
     3121 cgatgtaagg cgctgcgtaa gagatgtgcc ggtaagcctc ggctgtggga atttcgcttt
     3181 gcgcatcgaa aaaacggatg ctgcctttgc tgggcgtgag gtggccggag aggaggcgca
     3241 acagggtgga tttgcccgct ccgttggggc cggtgatggc ataagcatgg cccggcagca
     3301 gttggaggtc tgcctgccgg agtatccatt cgcggcgata ccgcttgccc atgccgttcg
     3361 tttcaatcct catgaacttt tacgccgttg agtttttgca cgcctttgac aaggccgcca
     3421 ttgcgccggg cctgttgcac gaagtccacg atttcctggc gttgttcggt aagcggcagc
     3481 agggtttcaa tttctccgag ggcatgactt gtattgtagc cgcgcacgaa gaggaggcgg
     3541 taaatgtcgg tgatgtggcc gatgagttct tccgggaact gccttctgga aagccctact
     3601 ttattgatgc ctacataagc caatggctct cgggctgccc tgatgtaggg gggcacatga
     3661 gtgcgcacca aagagccgcc ggcaataaaa ctgtggcgac caatgcgcac aaactgctgc
     3721 acggccacca agccttcgag gatggcgaaa tcgtcaattt ctacatgccc ggctaagttg
     3781 acgttgttgg ccaaaatgac atggttgccc aatatacaat cgtgggccac atgcacatag
     3841 gccatcagca ggcaatggct gcccactatg gtgcgtccgg cggccaccgt gccgcgattg
     3901 acggtgacat attcccgaat ggtcgtgtgg cttccaattt ctgcggtcgt ttcttcacca
     3961 ttgaacttca ggtcttgcgg tatagccgaa ataactgcgc cgggaaaaat gcggcaatgg
     4021 ctgccgatgc gggcgccgtc aaaaatggtg acatgcggac ctatccaggt gttgtcccca
     4081 ataactacat cgcgatcaat gtagcaaaaa gggccaattt caacgttttc accaatttgg
     4141 gcttcgggat ggatgtaaga ttgtgttgct tgcttattca ttattagagc gcttgacaat
     4201 ttgggctgtc agttctgcct ccgacacgat cttgtttcca acataagccg tgccgagcat
     4261 ctgacatatt ccccggcgaa tgggcgccat caactccaat ttcaggagca gggtatcgcc
     4321 gggaactact ttgcttttaa atttggcatt atcaattttc aaaaaatagg tatcccactg
     4381 tccggggtct cccatgatag acatggccag aatgccgccg gtttgagcca tggcctcaat
     4441 ttgtaagacg ccaggcatga cgggattgtt ggggaagtgc cccaaaaaaa actgttcgtt
     4501 aaaggtaacg tttttcaccc ccacaacgtg cgtatccgac agctcaataa ttttgtccac
     4561 caacaacatg gggtagcggt gggggagcca ttccgctatt tgctgtgtat taaacagggc
     4621 ttctttatcg gggtcatact taggttttcc tttgagtttg cgctgttcca gcatggcctt
     4681 tcgcagtaat cgggtgaatt ctacattggc tctgtgcccc ggcttgatgg cggtgatttt
     4741 tcctttaatg ggtttaccca aaagcgctat atcgcctatg acatccaata acttatggcg
     4801 ggcaggttcg tttttgaatt gcaaatccag cgtatnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gcctttgatg
     4981 aggttttgac tgaacagata ctccacttcg tgcaggaaaa cgaaagtgcg gcaaggcgca
     5041 atttcctcgg cgtagttgtc taaattgctg atggtggcaa actgctgccc caataccggc
     5101 gagttgaaat caatcaatgt tgttacctca aaacaatcgg acggcatggc caccaattcc
     5161 gagccggtca ccggatcgcg ataagtgatg ggttcagtga tctcaaaata ctcgcgttct
     5221 tcaatttgtt cctctatacc tgcctcgcga agggcttcta cgaaataacg cgcactgcca
     5281 tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 ccggtgagcg ccgaaagggc gtgttctacg gtgtgtactt cggcctcatc tacttgcaat
     5401 gtggtgccgc gctgggtaga aacaaccctg gccacatcgg caggaataac gggctgatcg
     5461 ggcaagtcgg tgcgctgaaa acggtagccg tggttaggcc cggcaggaat aaactgtaaa
     5521 ttgacctgcc tgcccgtgtg caggcccaca ccactaaggg caacttgtcg ttggatggtt
     5581 ctttgtttca ttttggaaag cgcttctgta aaaacagtcg gcaaaggtag ctattttttt
     5641 gggaggccat gctcatacaa taaaaaagcc gcgaaggcac tcccctcgcg gcattcatta
     5701 tcctccaata cttatcagaa ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg gctattcccg cgccaaaact
     5821 acccgtttcg ttttatcgag ccggccggca gatttgactt ttaccagata cattccgttt
     5881 tcaaatgaag ccagattcat ggtttcaatc acatactgca cctcctttat ctctctgaat
     5941 tgcaacaact tgccctcgat gctgtacacc tctatccgca catcgcgacc gacatactgc
     6001 gtcaaatcca cat
//
LOCUS       DAORLF010000297         5995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7184_length_5995_cov_2.551010, whole genome shotgun sequence.
ACCESSION   DAORLF010000297 DAORLF010000000
VERSION     DAORLF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7184_length_5995_cov_2.551010"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="PK198_19385"
     CDS             <1..1610
                     /locus_tag="PK198_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40967.1"
                     /translation="GRVGHLLETERHLWLTEVQDGTAWEVEMRISPSKVQQASCECAA
                     FRADKQCAHLAAALLALRRLLTDRQAEKQAARAQKKERPARSLDTQSILNNVRPDDLA
                     DFVRQYARSNRAFAVALKTRFASEVPALDNKAKFSELLDSAVGMARKADNSISYRGAG
                     KIASVADEMLAQARQATALRHWADAADMAQSIIEKLTPLLRKAGERQVLLRESIRQAF
                     QVLFDIPMTQAPPDLTDGLWNYAAEESKKLLYRNNELDRPFFRLLAALCTSPDKDEEL
                     RVLLSAQIDRYLNENRDPSALVLLQLNRLEHLGREIEARQLVETYLSAPDVLLFAVQQ
                     AAGREDFQQMVMLAEEGLRRNPPPHIAAELEDRLLLDAVRRNDMSKVSQLARTRYYKT
                     LEIKYYQALRTATARPVAEEVLEYLRKLPFSPRKRQAIAGVLSEEGRREELLAYLREC
                     RSPDLLAEFGPLLLPEKSNDILELYRLVLEQYLRGHVGPKPSQRIRQMVEQLYAKGAS
                     SIAEPLVAMIREAYPERHTLQEELQHL"
     gene            1607..2080
                     /gene="rlmH"
                     /locus_tag="PK198_19390"
     CDS             1607..2080
                     /gene="rlmH"
                     /locus_tag="PK198_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRF40968.1"
                     /translation="MKVELWAIGKTNERYLEEGMALYEKRLKHYLPFQWTVLPDIKGA
                     GNLSAQQLKAKEGESILSRLKDDDLLILLDERGRSFSSEGFATYLDKKLQQSQRRLVF
                     LIGGAYGFSDALYERANDQLSLSSMTFSHQMVRLFFLEQLYRAMTILRNEPYHNS"
     gene            complement(2143..3066)
                     /locus_tag="PK198_19395"
     CDS             complement(2143..3066)
                     /locus_tag="PK198_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF40969.1"
                     /translation="MSKNIFLLLGLTALIGLSSCVSKKKFTELQAGNERLQLTYEETK
                     LRLDECLSASRNYQDEIRRRDVEIGAKNSELEAKSRMVQQLEGQVDNLQRTNAGLLDR
                     MADLSVISKEGAESIKKSLESINAQTKYIQELTSSIQSKDSLNLALVMNLKRSLSDIN
                     DQDIQVEVRGSVVYVSIADKLLFKSGSAEISPRAESVLAKVAKVINDHKELNVMVEGH
                     TDNVPISNSCVADNWDLSVKRSTAVIRVLQGRHGVAADRLTAAGRSEFVPKTTNATAD
                     GRSTNRRTEIILTPKLDQFFKLLEAAPAGGK"
     gene            complement(3132..3782)
                     /locus_tag="PK198_19400"
     CDS             complement(3132..3782)
                     /locus_tag="PK198_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRF40970.1"
                     /translation="MIKKLTILWAFTALAAGTATAQDIEPVNTLFDTSAWIELVTIDP
                     GIQLDLKYATTDNFVKEKLYDCPRCFLRSDVAMAVRNVQKDLRRKGYGLKMLDCYRPL
                     PIQWKLWNKVPDPRYVADPRKGSMHNRGSAVDLTLVDAQGKELDMGTPFDFFGQEAYH
                     DYTNLPKEVLARRTLLKETMIKHGFRSTRTEWWHYSYTKRSYPISKMEWPCDGKGK"
     gene            complement(3787..4314)
                     /locus_tag="PK198_19405"
     CDS             complement(3787..4314)
                     /locus_tag="PK198_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40971.1"
                     /translation="MNSRHALPGISKGLIFSFFLLMQLGFCIPVEAQVFMQIERSGRP
                     KAQRIYPGEVIQYRYQGQWYAGEIQELLFDVNMVVLTRHTVRLSDIEALRYQRRWPKP
                     VGSQIALFGLSWSAWALIGTATDNEPGLDYRWSDAAVTATAVGAGLALPALVKKYRIV
                     EMGKKRRLRILDLNF"
     gene            complement(4347..>5438)
                     /locus_tag="PK198_19410"
     CDS             complement(4347..>5438)
                     /locus_tag="PK198_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRF40972.1"
                     /translation="ERPAIQTVFNAHISQVKELQPGHVLIIKRNGQVSEKPFIEARPR
                     AACSFERIYFSRGTDHDIYMERKKLGDQLAETVLQSVAYDFKHTVFAFVPNTAETAFI
                     GLMEGVERVLNRIKQEKILKLQTDENGLNTKKLEKILAMRVRQEKLVVKDAKIRTFIA
                     DDTQRSELVSYVYDVTYGLVQNERDTLVLLDDSIVRGTTLRDSILQIASRLRPKKIII
                     VFSAPQIRYPDCYGIDMSKLKEFVAFQALVQLLRETGQEQALHDAYARCKAAENLPAD
                     QMRNELQPVYELFTQEELSAKIADIVTPANIKPKVEVIFQTVEGLQNACPDNNGDWYF
                     SGNYPTPGGNRVVNRAFMNYMEKRDERAY"
     assembly_gap    5439..5448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5449..>5995)
                     /locus_tag="PK198_19415"
     CDS             complement(<5449..>5995)
                     /locus_tag="PK198_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRF40973.1"
                     /translation="RHFNAVPAEQLDDAVWLKNNKPYMGEVMLGHLRYGTHGDNTIET
                     CHPFLRASNWINRNLLLAGNFNLTNVDELFQELVELGQFPKEKSDTVTVLEKLGHFLD
                     DEVQRIHTWFKPDGYKNTEINDLIYDNLDLQRLLRRACKKFDGGYVMAGMIGYGDSFI
                     MRDPWGIRPAFYYADDEIVVAA"
BASE COUNT         1259 a         1566 c         1766 g         1394 t
ORIGIN      
        1 agggccgtgt agggcatttg ctggaaaccg agcgacacct gtggctcacc gaggtgcagg
       61 acggcacggc ttgggaggta gaaatgcgca tcagtccctc taaagtgcag caagcctctt
      121 gcgagtgcgc cgccttccgg gccgacaaac aatgcgccca tctggcggcc gccttgttgg
      181 ccttgcgccg tctgctgacc gaccgacaag ccgaaaaaca ggccgcccgc gctcaaaaaa
      241 aagaacgccc ggcccgcagc ttggacacgc agtccattct caacaatgtt cgccctgatg
      301 atctcgccga tttcgtgcgg cagtatgcgc gcagcaatcg ggcttttgcc gttgcgctca
      361 aaacccgatt cgcttccgag gtacccgcat tggacaacaa ggccaagttc tcggaattgt
      421 tggacagcgc cgttggcatg gcccgcaagg ccgacaacag catcagctac cggggagcag
      481 gcaaaattgc ctccgtagcc gacgaaatgc tcgctcaggc gcggcaggcc acagcgttgc
      541 gccattgggc cgatgctgcc gacatggcgc agagcatcat cgaaaaactg acgccactct
      601 tgcgcaaagc aggcgaacgc caggtgctgc tgcgcgagtc catccggcag gcatttcagg
      661 ttttgttcga cataccgatg acacaggcgc cccccgatct gaccgacggg ctgtggaact
      721 atgccgccga ggaaagcaaa aaactgttgt accgcaacaa tgaattagac cgcccttttt
      781 tcagactgct ggccgcgctg tgtacctcgc ccgacaaaga cgaggaactg cgcgtcttgt
      841 tgtcggcaca aatagaccgc tacctcaacg aaaaccgcga tccctcggct ctggtactgc
      901 tgcaactgaa ccgattggag catctgggcc gcgaaataga ggcccggcaa ttggtggaaa
      961 cctacctctc ggcgcccgat gtgctgctct ttgccgtcca acaggctgcc ggcagggagg
     1021 attttcaaca aatggtcatg ctggcagagg aaggactgcg ccgaaacccg ccgccgcaca
     1081 ttgccgccga attggaagat cggctcctgt tggatgccgt gcgccgaaac gatatgagca
     1141 aggtgagcca attggcccgg acgaggtatt ataaaacgct ggaaatcaaa tattaccagg
     1201 cattgcgcac ggcgacggcc cggcccgttg cagaggaggt gctggagtac ctgcgcaagt
     1261 tgcccttttc gccgcgcaaa cggcaggcca tcgcgggggt gctgagtgag gaggggcgcc
     1321 gggaggaatt gctggcctat ttgcgagagt gcaggtcgcc cgatttgctg gcagaattcg
     1381 gcccgctgtt gctgcccgaa aaatcgaacg acatcctcga actctaccgg ctcgttttgg
     1441 aacaatacct gcgcggacac gtcggcccca aaccctcgca gcgcatccgg caaatggtgg
     1501 agcagttgta cgccaaaggc gcctcctcca tcgccgagcc gttggtggcc atgattcggg
     1561 aggcataccc cgaacgccat accttacagg aagaacttca gcacctatga aagtggaatt
     1621 gtgggccatc ggcaaaacca acgagcgcta tctggaggaa ggcatggcgc tgtacgagaa
     1681 gcgtttgaag cactacctgc cctttcaatg gacggtgctg cccgacatca aaggcgcggg
     1741 caacttgtcg gcgcaacaac tgaaggccaa agagggcgag agcatcctct ccaggctcaa
     1801 ggacgacgac ctgctcatcc tgctcgacga gcgggggcgc tcattcagtt cggagggctt
     1861 cgccacttat ttagacaaaa aactccaaca gtcgcagcgg cgcctggtgt tcctcatcgg
     1921 cggcgcgtat ggcttttcag atgccttgta cgaacgggcg aatgaccaac tctcgctttc
     1981 ttccatgact ttttcccatc agatggtgcg tttgtttttt ttggagcaac tgtaccgcgc
     2041 catgacgatt ttgagaaacg agccgtatca taatagctga aaatgacgaa ggcccaacgc
     2101 tatataatgc gccgggcctc catgatatag aaatgaattt ccttacttcc ctcctgccgg
     2161 ggctgcttcg agcaatttga aaaactgatc gagcttcggc gtcaggatga tctccgtgcg
     2221 gcggttggta ctgcggccat cggcggtggc gttggtggtt ttgggtacaa actccgaacg
     2281 accggcagcc gtgaggcggt cggcagcgac gccgtgcctg ccctgcaaca cgcgaatgac
     2341 ggcggtagaa cgtttcacgc tgaggtccca gttgtcggcc acgcaagagt tgctgatggg
     2401 cacattatcg gtatggcctt ccaccatgac attgagttct ttgtggtcgt tgatgacctt
     2461 ggctaccttg gccagcacgc tttcggcgcg gggactgatc tcggcgctgc ccgacttgaa
     2521 gagcagttta tcggcaatgg agacatatac cacgctgccg cgcacttcta cctgtatgtc
     2581 ctgatcgttg atgtcgctga gcgagcgttt gaggttcatc accaaggcca gattgagcga
     2641 gtctttggat tggatactgg aggtgagttc ctgaatgtac ttggtttgcg cattgatgga
     2701 ttcgagtgat tttttgatgc tctcggcgcc ctccttgctg atgacggaga ggtcggccat
     2761 gcggtcgagc aggcctgcgt tggtgcgctg aaggttgtcc acctggcctt cgagttgttg
     2821 caccatgcgg cttttggctt ccagttcgct gtttttggcg ccgatttcca cgtctctgcg
     2881 gcgtatctca tcctgatagt tgcgggaagc gctgaggcac tcgtctaagc gcagtttggt
     2941 ttcctcgtag gtgagttgga ggcgttcatt gccggcttgc aactcggtga atttcttttt
     3001 ggaaacgcag gaactgaggc cgatcagggc cgtcaatccc agtaacagaa agatgttttt
     3061 tgacatagcg tagtgttttt ggtttgtaat tcaacaacat accacagtag ctcgttcgcc
     3121 tcgattctca cttatttgcc tttcccgtcg cagggccatt ccattttgga aatggggtag
     3181 ctgcgtttgg tgtaggaata atgccaccac tcggtgcggg tagagcggaa gccgtgtttg
     3241 atcatggttt ctttgaggag cgtgcggcgg gccagtacct ctttgggcag attggtgtaa
     3301 tcgtggtagg cttcctgtcc gaaaaagtcg aagggcgtgc ccatgtcgag ttctttgcct
     3361 tgcgcatcta cgagggtgag gtcaacggcc gagccgcgat tgtgcatgga gcctttgcgg
     3421 ggatcggcca catagcgcgg gtcgggcact ttgttccaaa gtttccattg gatgggcagg
     3481 ggccggtagc agtctaacat tttgaggcca tatcccttgc ggcgcaggtc tttttgtacg
     3541 ttcctgacgg ccatcgccac gtctgagcgg aggaagcatc gcgggcaatc gtacagtttt
     3601 tccttgacaa aattgtcggt ggtggcgtac ttcaaatcca actgaatgcc gggatcaatg
     3661 gtgacgagtt caatccaggc agaggtgtcg aacagcgtgt tgacaggctc tatgtcttgt
     3721 gcggttgccg ttccggcggc gagggctgtg aaggcccaga gaatggtgag tttttttatc
     3781 atggtttcag aaattaagat ccagaatgcg caggcggcgt tttttgccca tttctacgat
     3841 gcggtatttt tttacgagcg caggcaaggc caatcccgcg cctacggcag tagcggtaac
     3901 ggcagcatcc gaccagcggt agtcgaggcc gggttcgttg tcggtggcgg tgccgatgag
     3961 tgcccaggcc gaccaactga gaccgaacaa ggcgatctga ctccctacgg gtttgggcca
     4021 acggcgttgg tagcggagtg cctcaatgtc ggaaaggcgc accgtgtgtc gggtgagaac
     4081 taccatgttc acatcgaaaa ggagctcctg aatttcgccg gcataccatt gcccctgata
     4141 gcggtactga atgacttcgc cggggtagat gcgttgcgcc ttggggcggc cggagcgctc
     4201 tatttgcata aatacctgcg cctcaaccgg aatgcaaaaa ccgagttgca tcaaaagaaa
     4261 aaagctgaaa atcagccctt tggaaatgcc cggcaaggcg tgacgtgagt tcatatcaga
     4321 cagttattta cgaaagaacg cgtgtttcaa taagcgcgtt catccctttt ttccatgtag
     4381 ttcataaacg cccgattgac tacgcgattg ccgccggggg taggataatt tcctgaaaag
     4441 taccagtcgc cgttgttgtc cgggcaggca ttctgcaggc cctccaccgt ttggaaaatg
     4501 acctccactt tgggtttgat gttggccggc gtgacgatgt ctgcaatttt tgccgagagt
     4561 tcttcctgcg taaacagttc gtacacaggc tgaagctcat tgcgcatctg atcggccggc
     4621 aaattttcgg cggctttgca gcgggcatac gcgtcgtgca gagcctgctc ctgaccggtc
     4681 tcgcggagca attgcaccaa agcctgaaac gcgacaaact ccttgagctt cgacatgtct
     4741 atgccgtaac agtcggggta gcggatttga ggcgccgaga atacgatgat gatctttttg
     4801 ggacgaagac gagacgctat ttgcaggatg ctgtcgcgga gggtagtgcc gcgcacaatg
     4861 gagtcatcga gtagtaccag cgtgtcgcgc tcattttgta ccaatccgta agtgacgtca
     4921 tacacataag aaaccaactc ggagcgctgg gtgtcgtcgg caatgaatgt gcggattttg
     4981 gcatctttca ccacgagctt ttcctgacgg acgcgcatgg ccaatatttt ttccaacttc
     5041 ttggtattca gtccgttttc gtctgtttgc agcttcagaa ttttttcctg tttgatgcga
     5101 ttgaggacgc gttccacccc ttccatcaga ccgatgaagg ccgtctcggc cgtattgggc
     5161 acaaaagcaa aaacggtgtg tttgaaatcg taggccacag actggagcac ggtttcggcc
     5221 aattgatcgc ccaatttttt gcgctccatg taaatgtcgt ggtcggtgcc gcgcgagaaa
     5281 taaatgcgct cgaaagagca ggccgcgcgg ggccgagcct caataaacgg cttttcactc
     5341 acctgcccgt tgcgcttgat gatgagcaca tggccgggtt gcaattcttt gacctgcgaa
     5401 atgtgggcat taaaaaccgt ctgaatggcg ggccgttcnn nnnnnnnngg cagccaccac
     5461 gatttcgtcg tcggcatagt aaaaagccgg gcgaatgccc caggggtcgc gcatgatgaa
     5521 ggaatcgccg tagccgatca tgccggccat gacatagccg ccgtcaaatt ttttgcaggc
     5581 gcggcgcagc aggcgttgca ggtctaagtt gtcgtaaatg aggtcgttga tctcggtgtt
     5641 tttatatccg tcgggtttga accaggtatg aatgcgctgc acttcgtcgt cgaggaagtg
     5701 gccgagtttt tcgagtaccg tcaccgtgtc ggatttttct ttgggaaact ggccgagttc
     5761 gacaagttcc tgaaaaagtt catccacatt ggtgaggttg aaattgccgg ccagcagtag
     5821 gttgcggttg atccagttgc tggcgcgcag gaaggggtgg caggtttcga tggtattgtc
     5881 gccgtgggtg ccgtagcgca ggtggcccag catcacctcg cccatgtagg gtttgttgtt
     5941 tttgagccag acggcatcgt cgagttgttc ggcgggcacg gcgttgaagt gcctg
//
LOCUS       DAORLF010000298         5994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7186_length_5994_cov_1.942415, whole genome shotgun sequence.
ACCESSION   DAORLF010000298 DAORLF010000000
VERSION     DAORLF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7186_length_5994_cov_1.942415"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..253)
                     /locus_tag="PK198_19420"
     CDS             complement(41..253)
                     /locus_tag="PK198_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40974.1"
                     /translation="MTNKDLLELALLEAKSENIGAEMLLQYQVLCLPENFAKGDDELI
                     DAEDSLNFSKQLKLNNIKCATSYNGS"
     gene            complement(347..2185)
                     /locus_tag="PK198_19425"
     CDS             complement(347..2185)
                     /locus_tag="PK198_19425"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HRF40975.1"
                     /translation="MAKNIEPTLKLISEYLKLKKPENFVIPEYQRGYSWDITQCDKLW
                     QDIEAFIASNASDPYFFGTIIVDCSVTDKFSLIDGQQRTTTFFLLLKALLIRLNEAIT
                     RVPEDEESEALKAGLKAKRNKVMAILYKAEDEEIPSMLKDYTKTQNILILENKSINEL
                     YSDEVKKIIEAIDYRTSEQNVHKIPRKQKDNKYTNHFRNFKFFFSKLGEKSESQINQF
                     ATIFLQNCQVIEIRSWQVEQAITMFNSLNSTGMPLSDADIISAQLYSNAGDNKAIFNE
                     QWEQINKLANELSTRRIIDIDGVLQQFMYINRSINKEYISIKEDGNESVDVTTPGLRR
                     YYTDIKKELLREPIVLCGNLSKITKIWNDVKDYPAVKLLLRCNENAKLFLSGYLFRYE
                     TTEISEEIVSEVCECLLRLFTVLELSDFGYSSSKFKTFLFSENIKFVDKNVSIEAIKN
                     SFNEHIANNWNEKSIEDSIIEYDKNLLVFLNEYIYSKSKGVKFDFAENVNIEHIMPAS
                     GRNITTIQQDAGITDKEEFNGTVNKLGNKILLEEDINKSISNEWFKTKKQNSIISKSG
                     YKDSRYAIALSLTSYPKDTWTKEDIDTATQKAVKRIVKFIFNKQDD"
     gene            complement(2775..>5531)
                     /locus_tag="PK198_19430"
     CDS             complement(2775..>5531)
                     /locus_tag="PK198_19430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF40976.1"
                     /translation="GPGYSRLLRPVQASHPDGFYNQDIQLSLTSPDPGAVIRYTFNGS
                     EPSAASPLYQSPLFVESRIGQDNVISLIPTNGNPDPGPPFFEGWQPPAGEVFKINVIR
                     ARAFHPDAPDGPVQTFTYIVDPAAETRYSLPVFSLSTDNKNLFDPEIGIYVPGFFNNM
                     FQDGWERPAHLTFFENNGQLAFKENVGIQLNGNTTRSRPRKSFRIVMRSEYGSEELDY
                     QLFPDKNLDRFKRFLLRNSGNDWDFTVFRDGLFQSLAKQFRVETQYYRPSILFINGEY
                     WGIHNIRDKYDEHYFETKYGIPENELTVLENNALPKWGNPDGKAHYDALVNFVTNNNL
                     QNAANYQQVLQRMDVESFMDFQLTHIFVKNTDWPGNNALYWRYLRNDFAPAAGLRDGR
                     WRWMILDTDFGFDLPFFYVPGLEDGAAHNTLNFATEPSGPGWPNPSWSTLLLRKLLDN
                     SAFRLQFINRYCDLLNTAFSSAHLTSAINSISDQLSPEMAEHSARWRRPTSVSEWNNN
                     VQVLRNFAAQRPGFQFQHLRGKFGLAAPAELTLHVSDAAAGHIRLNSIDIAPSTTGVP
                     PLPYPWTGKYFPGVPVTARAIPQPGYRFSHWSGASGSTNAEIQINMSGATQLTAHFEP
                     EAPADLIHFWFFGNALPNDTPLESILPTFSVHPGAHLRFQSALAGYPFSEGHPLWRKA
                     SMERRNSPTAINYVPQGNNNQPFEQANMRGIQIKQPFTANGGENTLLFTASTEGFERI
                     RMQWAAKNEAAAERILVDYSTDDTGMWSNSGLSISSLPLSDDYQLLAVDFSNVPAANN
                     NPELKIRLRFGGNNLGADEGNRVTFNNMSLSGTAIMVSAEEPQSGGSRAILFPNPATE
                     TAYILHDDSIERWSLYDLSGRLLSSAQGSNEVPVAQLPAGLYWVKIWDVRGGVQVLKL
                     VRGE"
     assembly_gap    5532..5541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5542..>5994)
                     /locus_tag="PK198_19435"
     CDS             complement(<5542..>5994)
                     /locus_tag="PK198_19435"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF40977.1"
                     /translation="SCGPLAASGDLVINEVMASNAATVSDEDGDFEDWIELFNAGNTV
                     LDLSGYGLSDDPDSLFKWTFPQRSIAPNSYLLVWASDKNRRPASGPVHTNFKISAGNE
                     TVILTRPDGQRLDSLPILEQRTGIAYGRLPNGDEQWAYLVNPTPGAANT"
BASE COUNT         1350 a         1380 c         1540 g         1714 t
ORIGIN      
        1 ttttatttct cacaagccca cgccgcaaag cggcgattct tcacgaacca ttgtatgagg
       61 ttgcacattt aatgttattg agtttaagtt gcttgctaaa attgagacta tcctcagcat
      121 ctattaactc atcatctcct tttgcaaaat tttcaggcaa gcaaagcact tgatattgca
      181 gaagcatctc cgctcctatg ttctcgcttt tggcttctaa aagcgcaagt tcaagcaaat
      241 ctttattagt cattttttat ttttttaatt tctgacaacg ttactactgc cgacgtgccg
      301 acgcttagga ggcatgatag gcagtagatt gttggcagcg tatgttttaa tcatcctgct
      361 tgttgaatat gaatttcaca atccttttta ctgctttttg tgttgccgtg tcaatgtctt
      421 cctttgtcca ggtatctttt gggtaggagg ttagtgataa agcgattgca tatctgctat
      481 ccttgtaacc tgatttgcta ataatagagt tctgtttttt tgtcttaaac cactcattgc
      541 ttattgactt atttatatct tcttcaagca gtatcttatt tccaagtttg tttacagttc
      601 cattaaattc ttccttgtca gtaattcctg cgtcttgttg tattgtagtt atgttacgac
      661 cacttgcagg cattatatgt tcaatattta cgttttctgc aaaatcaaac ttaacccctt
      721 tgctttttga ataaatatat tcgtttagaa agacaagtag atttttatcg tattcaataa
      781 tcgaatcttc tatgcttttt tcattccagt tattagcgat gtgctcgtta aaactattct
      841 ttattgcttc tattgaaaca tttttatcaa cgaatttaat attttcgcta aataggaatg
      901 ttttaaactt agaacttgaa taaccaaaat cacttaattc taaaactgta aataatctca
      961 ataagcactc gcatacttcc gaaactattt cttctgaaat ttctgttgtt tcgtagcgga
     1021 acaagtatcc tgataaaaaa agcttagcat tttcattaca ccttaaaagc aattttacag
     1081 cagggtaatc ttttacgtca ttccaaattt ttgtgatttt agacaagttg ccgcataata
     1141 ctattggttc tcttaaaagt tcttttttta tgtctgtata atatcgtctt aagcctgggg
     1201 ttgttacatc taccgattca ttgccatctt ctttaatgct aatgtattcc ttgtttatgg
     1261 aacggttaat atacataaat tgctgtaaaa caccgtcaat gtctataatt cttcgagtac
     1321 ttaattcatt tgccagtttg tttatttgct cccactgttc gttaaatatt gctttattat
     1381 cacctgcatt agagtaaagt tgtgccgaaa ttatatctgc atctgaaagc ggcatacctg
     1441 tcgagttgag cgaattaaac attgtaattg cttgttctac ttgccaactg cgaatttcta
     1501 tgacttgaca gttttgtaaa aaaattgttg cgaattgatt aatctgcgat tctgactttt
     1561 ctcccaactt actgaagaaa aatttgaaat tgcgaaaatg attggtgtat ttattgtctt
     1621 tctgtttgcg tggaatttta tggacatttt gttcagatgt tctataatct atcgcttcaa
     1681 taattttctt tacttcatcc gaatacagtt cgttgataga cttattttct agtatcaaaa
     1741 tgttttgggt ttttgtatag tcttttagca ttgaaggtat ttcttcatct tcagctttat
     1801 acagaatagc cattacttta tttcttttgg ctttaagtcc agcttttaga gcttctgatt
     1861 cttcatcttc aggaactcta gtaatagctt catttaatct tatcaataac gctttaagta
     1921 gcagaaaaaa agtcgtggtg cgttgttgcc catcaatcaa actaaattta tcagttacgg
     1981 aacaatctac aatgattgtc ccgaagaaat atgggtcgct tgcattggaa gcaataaatg
     2041 cttcaatgtc ttgccacaat ttgtcgcatt gagttatgtc ccacgagtat ccacgttgat
     2101 attctggaat tacaaaattt tcaggctttt tcagtttaag gtactcactg attaatttta
     2161 gcgtaggttc aatgtttttt gccatttcgt tactttaatt tttctcgttt aatttcttcc
     2221 gttcacttgc tgccaacttg ttgtttgtcg aacttccctg ccatagcctc actgtcgtca
     2281 atccctcatc aggaagttgt atagacgaac ccaaggcgaa gttcatgttt tacttggtca
     2341 caggttcgtt aatatccgct atgggcgcat ccgcccggct accttcttcc caacctcaat
     2401 agaaacttcc tgcattcagc ttttccacag gattccattc tgaattcggt tgtgcaaatc
     2461 taatagaaga ttggcaaaaa tccaacggca cttcaaggaa aaatgttttt tttacaaact
     2521 gagtttgtca aacaaaaatc cctctcgccg aaatgccatc ttcctgttta ccacattccg
     2581 gcgatcaaag cgctccggcc ccgtattgcg tgagggggcc gaagagagga acgaccttag
     2641 gaggacgacc gaggcacccg acgccgctta tgattttacc cgtgcggcgg cacgcccccc
     2701 tcacccctca catactcaca tactcacata ctcacccact cacccctgac taacctcacc
     2761 tactcacccc tcacctactc acccctgact aacttcagca cctgcacccc gccgcgcaca
     2821 tcccatattt ttacccaata caagcccgca ggcagttggg ctaccggcac ctcgttgcta
     2881 ccctgagccg agcttagcag gcggcccgag aggtcgtaca agctccagcg ctctatgctg
     2941 tcatcgtgca gaatgtaggc ggtttcggtg gccggattgg ggaaaaggat ggcccggctg
     3001 ccgcctgact gcggctcctc cgcgctcacc atgatggccg tgccgctcag actcatgttg
     3061 ttgaacgtga cccggttgcc ctcgtcggcg ccgaggttgt tgccgccgaa acgcaggcgg
     3121 attttcagtt ccgggttgtt gttggctgcc ggcacattgg aaaaatctac tgccaacaac
     3181 tgataatcat cgctgagcgg gagggaggaa atgctcagtc cgctgttgct ccacataccc
     3241 gtatcgtcgg tcgagtagtc cacgaggatg cgctcggcgg cggcttcgtt tttggcggcc
     3301 cactgcattc ggatgcgctc aaacccctcg gtggaggcgg tgaagagcag cgtgttttcg
     3361 ccgccgttgg ccgtaaaggg ctgcttgatc tgtatgccgc gcatgttggc ctgctcgaag
     3421 ggctggttgt tgttgccctg cgggacgtaa ttgatggcgg tgggactgtt tctgcgctcc
     3481 atcgaggctt tgcgccacag aggatgcccc tccgaaaacg ggtacccggc caaggcggat
     3541 tggaaacgca gatgcgcacc cgggtgaacg ctgaaagtgg gcaatatgct ttccaaaggc
     3601 gtgtcgttgg gcagcgcatt gccgaaaaac cagaaatgga tgagatcagc gggcgcttcg
     3661 ggttcaaaat gcgcggtcag ttgcgtggcg ccgctcatgt tgatttgtat ctcggcattg
     3721 gtgctgcccg atgcgcccga ccaatggctg aaccggtaac cgggctgtgg tatggcccgc
     3781 gctgtgacgg gtacgccggg gaagtacttg ccggtccagg ggtagggcag gggcggcacg
     3841 ccggtggtgg agggcgcaat gtctatgctg ttgaggcgga tgtgcccggc ggcggcatcc
     3901 gagacgtgga gcgtgagctc ggcgggggcg gcgaggccga acttgccgcg caggtgctgg
     3961 aactgaaaac cggggcgctg cgccgcgaaa ttgcgcaaca cctgcacgtt gttgttccac
     4021 tcggacacag aagtggggcg gcgccagcgg gcgctgtgct cggccatttc gggactgagt
     4081 tggtcgctga tggagttgat ggccgatgtg aggtgcgccg aactgaaggc cgtgttgagc
     4141 aaatcgcagt atctgttgat gaattgcagt cggaaagcac tgttgtccaa tagtttgcgc
     4201 agcagcagcg tggaccagga gggattgggc cagcccggcc cggaaggctc ggtggcgaag
     4261 ttgagcgtgt tgtgcgcggc gccgtcttcg aggccgggca cataaaaaaa cggcagatcg
     4321 aagccgaaat cggtgtcgag gatcatccag cgccagcgac cgtcgcgcag gccggcggca
     4381 ggtgcaaaat cgttgcgcag gtagcgccag tagagggcgt tgttgccggg ccagtcggtg
     4441 tttttgacaa aaatatgcgt cagttggaaa tccataaaac tctccacgtc catgcgttgc
     4501 agcacctgct ggtaattggc cgcgttttgg aggttgttgt tggtcacaaa attcaccaaa
     4561 gcgtcgtaat gcgccttgcc gtcgggattg ccccatttgg gcagggcgtt gttttccagc
     4621 acggtcagct cgttttccgg gatgccgtat ttggtttcaa aatagtgctc gtcgtatttg
     4681 tcgcggatgt tgtgaatgcc ccagtattcg ccgttgatga agagtatgga aggccggtaa
     4741 tactgtgttt ccacgcgaaa ttgtttggcc aaggactgaa acaggccgtc gcgaaagacg
     4801 gtaaagtccc agtcgttgcc ggaattgcgg agcaggaaac gcttgaagcg gtcgaggttt
     4861 ttgtcgggaa acaattgata atcaagttcc tccgagccgt actccgaacg catgacgatg
     4921 cggaatgatt tgcgcgggcg actgcgggtg gtgttgccgt tgagttggat gccgacattt
     4981 tctttgaagg ccaattgacc gttgttttca aaaaaagtga gatgcgcggg ccgctcccag
     5041 ccgtcctgaa acatgttgtt gaagaagccc ggcacataga tgccgatttc cgggtcgaag
     5101 aggtttttgt tgtcggtgct aagtgaaaaa acgggcagcg agtagcgggt ttcggcggca
     5161 gggtccacga tgtaagtgaa agtttgcacg gggccgtcgg gcgcatcggg atgaaaggcc
     5221 ctggcgcgta tcacattgat tttgaacact tcacctgcgg gcggctgcca gccttcgaag
     5281 aacggcgggc cgggatcggg gttgccgttg gtgggaatga gggagataac gttgtcttgt
     5341 ccgatgcgac tttccacgaa aagcggcgac tggtacagcg gcgaggcggc gctgggttcg
     5401 gagccgttga acgtgtatcg aatgacggcg ccgggatcgg gcgaagtgag gctcagttgg
     5461 atgtcctggt tgtaaaaacc gtcgggatgc gaggcttgga cggggcgcag caacctgctg
     5521 taacccggcc cnnnnnnnnn nggtattggc agcgccgggc gtgggattga ccaagtaggc
     5581 ccattgttcg tcgccattgg ggaggcgtcc gtaggcaatg ccggtgcgtt gttcgaggat
     5641 gggaagcgag tccaagcgtt gtccatcggg gcgggtgagg atgaccgttt cgttgccggc
     5701 agatattttg aaattggtat gtaccgggcc agaagccggt cttctgtttt tgtcggaagc
     5761 ccagaccagg aggtagctgt tgggggcgat gctgcgctgc gggaaggtcc atttgaacag
     5821 gctgtcgggg tcgtcgctga ggccgtaacc gctgagatcg agcacggtgt tgccggcgtt
     5881 gaacagttct atccaatcct caaaatcacc gtcttcatcc gacactgtgg cggcgttgga
     5941 ggccatgact tcgttgatca cgaggtcgcc agaagcggcg agcggcccgc agct
//
LOCUS       DAORLF010000299         5940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7258_length_5940_cov_1.599660, whole genome shotgun sequence.
ACCESSION   DAORLF010000299 DAORLF010000000
VERSION     DAORLF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_5940_cov_1.599660"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PK198_19440"
     CDS             <1..232
                     /locus_tag="PK198_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40978.1"
                     /translation="PVSVSTAELFVQIDAGTEEIVPAVGVPEHILGLVPLNSSAPISG
                     AKPLGRASPSISVVTPTVAPPDSSRVLVAAGM"
     gene            complement(306..>729)
                     /locus_tag="PK198_19445"
     CDS             complement(306..>729)
                     /locus_tag="PK198_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40979.1"
                     /translation="TGGLAFSTGFGIYGIRINAGTGHSVYHNSVHLFGLRAGTANSSL
                     LTAALGLINTAQTGCDIRNNILSNTISGGTTSVANVSIYLPSGASSSLNLILNNNAYY
                     SGTDAARQGVAQVGTTAGTGLMHQALRLLPTFGHIPAL"
     assembly_gap    730..794
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<795..>1147)
                     /locus_tag="PK198_19450"
     CDS             complement(<795..>1147)
                     /locus_tag="PK198_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40980.1"
                     /translation="MSGLTISNNAVNACARGIAVQGSATSVVNTLTVTNNVVGPAASG
                     STTSVYAAGITLQGFAAAQVSGNTIQNMESYLAQTIWALDFGSVSATGTNAVVEKNTI
                     QKVNLQNSGTYGVYG"
     assembly_gap    1148..1199
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1200..1505)
                     /locus_tag="PK198_19455"
     CDS             complement(<1200..1505)
                     /locus_tag="PK198_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40981.1"
                     /translation="MIEFNGADNVTLDGDNPNSAGINRNLTITNTAANTVNYTMVVRV
                     AVATSTITSADNIVIKNCNVNGSGTGRNISSANSSTGTENTTYGIYAGGGASTSSQTT
                     "
     assembly_gap    1539..1581
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1582..2305)
                     /locus_tag="PK198_19460"
     CDS             complement(<1582..2305)
                     /locus_tag="PK198_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40982.1"
                     /translation="MRSFTLLFMLGFIAFTAQAQSGIFESYIILDAGSGNTYYDLQAT
                     TGNPDFTGSLGSFVQCAGTLTLNGAQNKTIKCPGDDITNGAIWYRVYPTGSPSGAFVS
                     VNLPFAANLGSGCNGNDQQWETSAANVNLLSNRTPGNYTLEVYTTSDFTYTGGGGGSG
                     THFSNNGGANYSATFTVTGGATCPVFVTASGATAFASYTTLKGAFDAVNAGTHTGAIT
                     IDIAANTTETAAGVLNSSGAGSA"
     gene            complement(2495..>3076)
                     /locus_tag="PK198_19465"
     CDS             complement(2495..>3076)
                     /locus_tag="PK198_19465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF40983.1"
                     /translation="EAARQQEFLGILVSESERISRLINQVLDLEKLSSSASGGHKEII
                     DFAEIVQKSYLGLSRLMEEKDISAKLQIPNQPTTISGHRDRLTQVVVNLLSNAIKFCD
                     AEAGSIKVTLTRESNRVRLTVSDNGPGIPAAQRAHIFERFTQLPGADGSKPQGSGLGL
                     FISRTIVEDHGGQIGVEEGEDGGAMFWVELEQL"
     assembly_gap    3077..3089
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3090..5216)
                     /locus_tag="PK198_19470"
     CDS             complement(<3090..5216)
                     /locus_tag="PK198_19470"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF40984.1"
                     /translation="MSAWLIVIVFICYMSLLFGVAFWAERRARQGRSVVSSPVVYSLS
                     LAIYCTAWTYFGSVGRASVQGLGFLPIYLGPTLVAPLWIMVLKKMIHISKNQRITSVA
                     DFISARYGKSAGLGIAATIIAVVGIIPYISIQLKAIVSSVEVLMGPAFQRGGPIYLDV
                     GLYVAIALAVFAILFGTRRLDPNEHHEGLIAAIAFESVVKLIAFLAVGVFVTFVLYDG
                     FGDLFSKGATVPDIAKLFRFESSGIDGGQWFWLIVLSMSAILFLPRQFHVAVVENTDP
                     GFVNKAAWLFPLYLLLINIFVLPIAVGGLLEFPTGIVDPDTFVLSLPLSAGHDTLALL
                     AAVGGFSAATGMVIVSVLALSIMISNNLVLPLLLQPSILQRNDFTDLSPRLLGIRRVS
                     IVIVLLMAYSYFKSVGAQYALVSIGLISFTAAAQFAPLIIGGIYWKRATRKGAMAGLL
                     AGFVVWTYTLPLPTLVETGVLPESLMTEGLWGLNWLKPHNLFGLTGMDPISHGAFWSL
                     LFNIALFSIVSVYTQQSALGISQADIFVNIYKYREGPSDYEMLRRQAKTRDLRLLLQR
                     FLGQSKADVLLDEYSEANGVDLNKLREAPPDLVNYVEVQLSGAIGAASAQLIISSVSK
                     EDPISLEEMFEALEQTREALAYSKALEEKSAELEQMTQQLQTANEQLQELDRLKADFI
                     TTVTHELRTPITAIKALAKILMDHPHL"
     gene            complement(5213..5440)
                     /locus_tag="PK198_19475"
     CDS             complement(5213..5440)
                     /locus_tag="PK198_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40985.1"
                     /translation="MQQRRTKLIGISIIGLILLNFPIAGLFSRNYTLWGIPGLYAAFF
                     LVWLGLIFLIKHYADPARKRTARPSKPPAAP"
     gene            complement(5522..5746)
                     /locus_tag="PK198_19480"
     CDS             complement(5522..5746)
                     /locus_tag="PK198_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40986.1"
                     /translation="MSKFRFGLGPSASNVVVVLYVFGTLLMRFLLEQQLQGRFVISLA
                     MGAFALLFLWAMIRSKVLNPTLLGLAEKIQ"
BASE COUNT         1398 a         1503 c         1531 g         1335 t
ORIGIN      
        1 gccggtaagc gtaagtactg ctgagctatt tgtacaaata gacgccggga ctgaagaaat
       61 cgtgccggca gtgggcgtac ccgaacatat attaggattg gtaccgctaa attcgtcagc
      121 gccaatatcc ggggctaagc cattggggcg ggcatctcca tcaatatcgg tggtgacgcc
      181 tactgtagcg ccgccggatt cgagcagggt gctggtagct gccgggatgt gaaggtccgt
      241 tgctgaggta tatggtacgg tagtggtggt tgcaaaagat gcattgtcat tggcgccacc
      301 tgcgtttaaa gtgctggtat atgcccgaag gttggcagca ggcgaagtgc ttgatgcatt
      361 aaaccagtac ccgcagtagt ccctacctga gctacgcctt gtcgtgccgc atcggtaccg
      421 gaataataag cattgttatt caaaatcagg ttcagggaag aactcgcccc tgaaggaaga
      481 tagatgctca cgttggcaac gctcgttgtg cctccggaaa tggtattgga taatatgtta
      541 ttgcggatat cgcaacctgt ttgcgctgta ttgataaggc ccagggcagc agttaaaagg
      601 cttgaattgg ccgttcctgc tctcaaccca aaaaggtgga ctgaattgtg ataaacggaa
      661 tggccagtac ccgcattaat ccgaatacca tatatgccaa aaccagtgct gaaggcaagt
      721 ccacctgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnatgcca taaacgccat aagttccaga gttttgaagg tttacttttt
      841 ggatggtatt tttttcgacg actgcatttg tacccgttgc cgaaaccgaa ccaaagtcaa
      901 gcgcccagat ggtctgagcc agataagact ccatgttctg aatggtattg cccgaaactt
      961 gtgcagcagc aaacccctgc agggtaatgc ccgcagcata aacactggtg gtgctgcccg
     1021 aagcagcagg gccaacaaca ttattcgtta ccgtcagtgt attcacaact gaagtcgccg
     1081 aaccttgtac cgcaatgccc cgtgcacacg cattcacggc attgttgctg atggtcagac
     1141 cgctcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1201 gttgtttgtg aagaagtaga agcgccgccg cctgcataaa taccgtaagt cgtattttct
     1261 gtaccggtac tggaattagc gctggaaatg tttcttcccg tcccgcttcc gttaacattg
     1321 cagtttttga tgacaatatt gtcggcagag gtgatggtcg aggtggccac agctacccgg
     1381 acaaccatcg tatagttaac cgtattggcg gccgtatttg taatggtcag attccgattg
     1441 attcctgccg aattggggtt gtcgccatca agggtcacgt tatctgcgcc gttgaattcg
     1501 atcaatcccc ggcctgttac cgttggccct gaaaccgtnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn naagccgaac cggcgccgct gctgttcaat actccagccg
     1621 ccgtttctgt ggtatttgct gcgatatcaa tcgttattgc ccccgtatgc gtaccggcat
     1681 tcacagcgtc aaatgcgcct ttcagcgtcg tatagctcgc aaacgccgtg gccccgctcg
     1741 ccgttacaaa taccgggcag gtagcgccgc cggtcaccgt aaatgtagcg ctgtagttgg
     1801 cgccgccatt gttggagaaa tgcgtgccgg agccgcctcc accgccggta taagtaaaat
     1861 cggacgttgt gtaaacttct aaagtatagt tgccgggcgt gcgattggac agcaggttga
     1921 cattggcggc actcgtctcc cattgctggt cattgccgtt acaaccgctg ccgaggttgg
     1981 cggcaaatgg caggtttacc gaaacaaaag ctcccgatgg acttccagtt ggatagaccc
     2041 gataccaaat cgcgccattg gtgatgtcgt cgccggggca cttgatggtt ttgttttgtg
     2101 cgccgttcag tgtcaatgtg cctgcacatt gtacgaacga tcccaaactt cccgtaaaat
     2161 ccgggttgcc ggtcgttgcc tgcaggtcgt agtatgtatt tccactaccg gcatcgagaa
     2221 tgatgtagct ctcaaatatt ccgctttggg cttgcgccgt aaatgcgata aaaccgagca
     2281 tgaataaaag tgtaaaactt ctcatacgaa ataggatttt aaggtaagta gattgcaaat
     2341 gtagcaattt ggtgtgaagg gattccttcc tggcattttt tctttttaga aaaaacaccc
     2401 aaaggaaact gctgtttcgg tgtgcctgat tggcaaaaaa attggcgcag cgccgttgga
     2461 ctacaatctc ttattttgag gattaatttt cgtgttatag ctgctccagt tccacccaaa
     2521 acatcgcccc gccatcttcg ccttcctcta cgccgatctg cccgccgtga tcctccacga
     2581 tggtgcgcga gatgaacaag cccagcccgc tgccctgtgg tttggagccg tcggcgccgg
     2641 gcaattgcgt gaagcgctcg aagatgtgcg ccctttgcgc ggcgggtatg ccggggccgt
     2701 tatcggacac cgtgagacgg acgcggttgc tctcccttgt cagggtcacc tttatcgaac
     2761 cggcctccgc atcgcaaaac ttgatggcgt tcgacaacag gttgaccacc acctgtgtga
     2821 ggcggtctcg gtggccggaa atggtagtgg gttggttggg tatttgcagc tttgcggaga
     2881 tgtccttttc ttccatcagg cggctgaggc cgaggtagga tttttgtaca atttcggcaa
     2941 aatcaataat ttctttatgg cctccggatg ccgacgaact tagtttttcc aaatccaata
     3001 cctgattgat gagcctgctg atgcgctcgc tttcgctgac gaggatgccg aggaattcct
     3061 gttgccgcgc agcttcnnnn nnnnnnnnnc agatgggggt ggtccatcag gattttggcc
     3121 aatgctttga tggccgtgat gggggtgcgc agttcgtggg tcacggtggt gatgaagtcg
     3181 gctttgaggc ggtccaattc ctgcaattgc tcattggccg tttgtagttg ctgcgtcatc
     3241 tgttccaatt cggccgactt ctcctccagc gccttgctgt acgccagcgc ctcgcgggtt
     3301 tgctctaatg cctcaaacat ctcctccaga ctgatggggt cttctttgga aacagaactg
     3361 atgatgagtt gcgccgatgc cgcaccaatg gcgccgctga gttgtacttc cacatagttg
     3421 accaaatcgg gaggcgcttc tctcagttta ttcaaatcca cgccgttggc ttcgctgtat
     3481 tcgtccagca aaacatctgc tttggactgc cccagaaaac gctgcaacaa gagccgcaaa
     3541 tcgcgggttt tggcctgccg ccgcagcatt tcgtagtcgc tggggccttc ccggtatttg
     3601 taaatattga caaaaatatc ggcctgcgaa atgcccagtg cgctctgttg ggtgtaaacc
     3661 gaaacgatgc tgaacaaagc gatgttgaac aacaggctcc aaaatgcccc gtgcgagatg
     3721 ggatccatgc ctgttaggcc gaataaattg tgcggtttga gccaattcaa tccccacaaa
     3781 ccctccgtca tcaggctttc gggcagaacg ccggtttcta ccaacgtagg caagggcagg
     3841 gtataggtcc ataccacaaa gccagccagc aagccggcca tagcgccttt gcgggtagct
     3901 cgtttccaat agataccgcc gatgatgagg ggagcaaact gcgccgctgc tgtgaacgaa
     3961 atcaggccga tggagactaa agcatactgc gcgcccacgg atttgaaata gctgtatgcc
     4021 atcaataaaa caatgacgat actgaccctc cgaatgccca gcagtcgggg cgagagatcg
     4081 gtgaaatcat tgcgctgcaa tatactgggc tgcaagagta aaggcaatac caagttgttg
     4141 gaaatcatga tactaagcgc cagcacagac acgatgacca tgcccgttgc cgccgaaaaa
     4201 ccgcctactg ccgccagcaa tgccagggtg tcgtgtccgg ctgaaagcgg caagcttagt
     4261 acaaaagtgt cggggtcaac gatacctgtg gggaactcca gcagaccgcc cacagctatg
     4321 ggcagcacga aaatattgat gagcaagagg tacagtggga agagccacgc cgctttgttg
     4381 acaaacccgg gatcggtgtt ttctactacc gccacatgaa attgccgggg caaaaacaaa
     4441 atggccgaca tggacaatac gatgagccaa aaccattgcc cgccgtctat gccgctgctc
     4501 tcaaaccgga acaatttggc gatatcgggc acggttgcgc ctttggaaaa caaatcgccg
     4561 aagccgtcgt acaacacaaa tgtgacgaat acgcccaccg ccagaaatgc gatcagtttt
     4621 accaccgact cgaaagcaat ggctgctatc aagccctcgt ggtgttcgtt ggggtctaag
     4681 cgccgggtgc cgaataagat ggcaaaaacg gccagcgcaa tggccacata caaacccaca
     4741 tctaaataaa taggtccgcc gcgctgaaat gcaggcccca tgagcacctc cacgctgctg
     4801 acgatggcct tgagttggat ggaaatgtac ggaatgatgc ccaccaccgc tatgatggtt
     4861 gcggcaatgc ccaaacctgc gctttttcca tatcgggccg aaatgaagtc tgccactgag
     4921 gtaatgcgct gatttttact gatgtggatc atttttttta agaccatgat ccagagcggc
     4981 gccactaaag taggtcccag ataaatgggc aaaaatccca gtccctgtac cgaggcccgc
     5041 cccacgctgc cgaagtacgt ccaggcggtg caataaatgg ccagagacag agaatacacc
     5101 acaggtgaac tgactacgct gcggccttgc cgggcacggc gctctgccca aaatgccacc
     5161 ccgaacaaca gcgacatata gcagatgaaa acgatcacta tgagccaggc actcatgggg
     5221 cggcaggcgg tttagagggg cgggcagttc ttttgcgggc aggatcagcg tagtgcttta
     5281 ttaggaaaat aagccccagc cagaccagaa aaaaagcggc gtacagcccc ggaatccccc
     5341 acaaggtgta gttccggctg aagaggccgg cgatgggaaa gttcagcagg atcaacccga
     5401 tgatgctgat gccgatgagt ttggtacgtc gttgctgcat ggtaaaagaa acccggaaga
     5461 cgtattagta cgcttccggg ctaccaaaga tatagattca aggaagaaaa tcaacacagt
     5521 ctcactgaat tttttctgcc agccctagaa gcgtcggatt cagcaccttg cttctaatca
     5581 tggcccacag gaaaaggagt gcaaaagcgc ccatcgccag cgaaatgacg aatcgcccct
     5641 gcaactgctg ctccagcaaa aaacgcatca gcagcgtgcc aaaaacatag agcaccacca
     5701 ccacgttgga ggccgaaggc ccaagtccga atctgaattt actcatgttc gttttttttg
     5761 ttcaaggttt cgggttcgcg tggcgaccgc caactgccta ccgcacaccg acttaatgcg
     5821 catgcgcctt gcctgcacca cgcggcaccc ggatgctttc gatcatttcc tgcactttga
     5881 gtggcggagc gggcgtcata cgggacacaa ccaaagtaac tacggcgttg atgaccatgc
//
LOCUS       DAORLF010000300         5939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7262_length_5939_cov_1.751530, whole genome shotgun sequence.
ACCESSION   DAORLF010000300 DAORLF010000000
VERSION     DAORLF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_5939_cov_1.751530"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..765)
                     /locus_tag="PK198_19485"
     CDS             complement(79..765)
                     /locus_tag="PK198_19485"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF40987.1"
                     /translation="MKNIRMMAMAAMCLLLSNLHAQKTIELDAFDAILASGNLEVVLI
                     PGDVERAVIEISGAPKDELSVKTVRGELRINYLNTLIYKNFEVKVTVWYKSLRAVRGN
                     AGARIGSETGITADRLELRAGSGAQVELNVEVRSLEAYASEGGLLRVGGKAESQDANA
                     TTGGRYEAFRLDCRNTYVRASLGGQAEVVALKKLEATAHTGGSIEYQGDPEQSDVRHI
                     ISGDIRRGSR"
     gene            complement(935..>1236)
                     /locus_tag="PK198_19490"
     CDS             complement(935..>1236)
                     /locus_tag="PK198_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF40988.1"
                     /translation="TDFLHFIRGFNQNEEDRFVNEARMRAYTAQDIMTTGLAKLNPND
                     RINVALELFMVNRFHAIPIVAEGNELVGILTTFDIIKAVSQEEVTPDQILDNQSK"
     assembly_gap    1237..1270
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1271..1372)
                     /locus_tag="PK198_19495"
     CDS             complement(<1271..1372)
                     /locus_tag="PK198_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF40989.1"
                     /translation="MSSKLITVNPEDKLSRIQEIFDQNTIHHLPVVRF"
     assembly_gap    1538..1609
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1610..1881)
                     /locus_tag="PK198_19500"
     CDS             complement(<1610..1881)
                     /locus_tag="PK198_19500"
                     /inference="COORDINATES: protein motif:HMM:NF020444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="element excision factor XisI family protein"
                     /protein_id="HRF40990.1"
                     /translation="MDKLTHYKQIAKQIIESVLKKLAQVKDYESLNVVDDNHGHYLVM
                     ADGWENGTRNYGPIVHIQVKEDGKVWLRYDGTDLEIGQELLDMGIAP"
     assembly_gap    1927..1941
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1942..2307)
                     /locus_tag="PK198_19505"
     CDS             complement(<1942..2307)
                     /locus_tag="PK198_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRF40991.1"
                     /translation="MNSIQLLSNHQLRKTQFRERVLDIFLRYERALSQKDLEEQLHES
                     DRITLYRTLKTFEEKGLIHKAIDGSDRLKYALCAGHCDVHAHHDHHAHFHCVTCERTF
                     CIEDVQAPALDAPPGFEVES"
     gene            complement(2316..>2760)
                     /locus_tag="PK198_19510"
     CDS             complement(2316..>2760)
                     /locus_tag="PK198_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40992.1"
                     /translation="HFQIQVLDKAGNDNPTAYVYAIKVLNAADTTPPALNISSPIVNS
                     GDMLVLRKGQNITFSGNVADNRPLAEGGNGKVEIFYRSNASGNTFRWGSPLVFTAADD
                     DYKDFSLTLPVPNTAANGDYVVTVWAYDGVNNAAPPIIYSARITN"
     assembly_gap    2761..2778
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2779..3150)
                     /locus_tag="PK198_19515"
     CDS             complement(<2779..3150)
                     /locus_tag="PK198_19515"
                     /inference="COORDINATES: protein motif:HMM:NF026758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HRF40993.1"
                     /translation="MKINSHTLLILAAFIGLLSSCGPKNVDDTAPTLAFIALSPEPTP
                     DFICGAEEQVVFHLRSGDTLSLDLRLSDDVELSQYKVDIHTNFDCHGHKMDTEDWLVL
                     QTGNLSGKEYLLQLRLPVPANV"
     gene            3237..>5290
                     /locus_tag="PK198_19520"
     CDS             3237..>5290
                     /locus_tag="PK198_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF40994.1"
                     /translation="MRLYITLLFLLYTLLAQAQISGKVVSERGETLPGALVLLLPDSA
                     STITDANGSFAFSNLRPGRYALEVSFLGFNIWRSELTLERKPLRVEAVLGSRDQLLET
                     VVVSADHAKQESTLSHEHVSLNFITQQRGGTLAQAIEKLPGLSAITVGTGIAKPVIRG
                     LSFNRIIVNSQGIKQEGQQWGADHGLELDQFDAEQVEIIKGPASLQYGSDGLGGVINI
                     LPGAIPQKNSWSGSLMGLYKSNNHHWAGSAFAALNRNDWFATARFSRQDYGAFRVPAD
                     RFVYNGFELPILDNTLTNTGGRENNFNLNLGRKTANSITRFTYSLYDLEAGLFPGAVG
                     IPRSYTIVPGLDPRAVSVPKQTVSHHKASLSRLMFWNENHAEVNIGYQYNLRREFSFP
                     ENHNRFLLSDPNDRLALQLDLQTWSANGHIQLQASEIWKTTYGFNAQYQTNRRAGFEF
                     LLPDFSSFRTGIFSIVEAEPTDRMVFTGGVRLDLAGNATQFFEQPFFDSRGNVLGYQR
                     AAATDQLFFNVSASAGLNYELIPENLTIKANAGKSFRAPYPNETSSDGVHHGNFRHEL
                     GTPDLRSEHGYQLDLGAEWESPHWEVAAAAYVNYFDNFIYLQPSGRFVFRPDAGQTYQ
                     YVQHDAVYTGFELDATWKLNRAWTLHGNGEYVWNLNLDEQRALPFTPPPSVMLELRLQ
                     PAR"
     assembly_gap    5291..5344
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5345..>5571
                     /locus_tag="PK198_19525"
     CDS             <5345..>5571
                     /locus_tag="PK198_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40995.1"
                     /translation="HYFFAKTSATVAQNEATTPAYNLVEAGISGSLPWGKHPPQWSLM
                     VQNLLNTSYFNALSRYRILNIPEQGRNVVLS"
     assembly_gap    5572..5710
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5719..>5939
                     /locus_tag="PK198_19530"
     CDS             5719..>5939
                     /locus_tag="PK198_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF40996.1"
                     /translation="MMTNSILHAFQQARLGGQKQIAVLVDPDKTSPDSAAVLARRASE
                     AGIHYLMVGGSLVVDGQFELCVEALRVHS"
BASE COUNT         1275 a         1504 c         1507 g         1321 t
ORIGIN      
        1 aagaagcgag ggtttctgtt tcaaaaaaaa ctccgcgtcg tcatccggcg cggagttttt
       61 tttattgttg aaataatact acctcgaacc ccgccgtata tcaccggaaa tgatgtggcg
      121 cacatcgctt tgttcggggt cgccctgata ctcaatggag ccaccggtat gcgcagtggc
      181 ctctaatttt ttcagtgcta cgacctcggc ctgcccgccc agagaagccc gcacataagt
      241 gttccggcaa tctaagcgaa atgcctcgta cctgccgcca gtagtggcgt tggcatcctg
      301 cgactccgcc ttgcctccca cacgcagcag gccgccttcg gaagcatacg cctccagaga
      361 gcgaacttcc acattcaact ccacctgagc gccggaaccg gcccggagtt ccaaccgatc
      421 ggctgtaatg ccggtttcgg aaccaatgcg ggcgccggca ttgccacgaa cagcgcggag
      481 gcttttgtac cacacggtca ctttgacctc aaaattcttg taaatcagcg tattgagata
      541 attgatgcgc agttcgccgc gcaccgtttt gacgctcagt tcgtccttgg gagcgccgct
      601 gatttcaatg acggctcgtt cgacatcgcc gggaatcagc acgacttcca gattgcccga
      661 agccaaaatg gcatcgaagg catctaattc aatggttttt tgagcgtgga gattggacag
      721 cagcaggcac atcgccgcca ttgccatcat tcggatgttt ttcataactt gttttttccg
      781 gtagatgcag gaaacgaagg tttaccccta ctccatccgt acatcaggca gaaatacaag
      841 gggagaaaaa aacgcctctg ccctggataa gaccagagga gaggcgtttg gaagaggaaa
      901 ttggaaagag aaagtaatct tattccataa gcctctattt gctttggtta tcaaggattt
      961 ggtcgggagt gacttcctcc tgtgaaaccg ctttgatgat gtcgaaagta gtgagaatgc
     1021 ccaccaattc attgccctcc gctacgatgg gaatggcgtg aaaacggttg accatgaaca
     1081 actccagcgc tacgttgatg cgatcgttcg gattgagctt ggccaagccg gtagtcataa
     1141 tatcctgcgc cgtgtaggca cgcatacgcg cttcattgac aaaacggtct tcctcgtttt
     1201 ggttgaaacc gcgaatgaag tgcaggaagt ctgtttnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn gaatcggacg acggggaggt ggtggatggt gttttggtca aaaatttcct
     1321 gaatccggga aagtttgtct tccgggttca cggtaatgag tttgctgctc attattgatt
     1381 taacgggtgc gagaatgttc atttgtaatc aagtttggtt agttatgtgt gaaataagac
     1441 aatgctaaat acagggacaa tttggccctt atgtagtcgc gcagccaatg atgccggtca
     1501 gtgcggcggc tgattttcat cataagaact tatgcagnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gagcaattcc
     1621 catgtccaat aattcctgcc caatttccaa atcggtaccg tcgtatctca accacacctt
     1681 gccgtcttct tttacttgaa tatgaacaat ggggccatag ttgcgcgtac cgttctccca
     1741 tccatctgcc ataacaaggt agtggccatg attatcatct accacgttaa gtgattcata
     1801 gtcttttacc tgagccagct ttttcaatac gctttcgatg atttgtttag caatttgctt
     1861 gtaatgagtc agtttatcca tgatacaatt tttttaatga gcgcactccg cacaacgccc
     1921 gttgatnnnn nnnnnnnnnn nactctccac ctcaaagccg ggaggcgcat ccaaggccgg
     1981 cgcctgcacg tcttcgatgc agaaggtgcg ttcgcaggta acacagtgga aatgagcatg
     2041 atgatcgtgg tgggcatgca cgtcgcaatg gccggcgcag agggcatact tgaggcggtc
     2101 gctgccgtct atggccttgt gaatgagacc tttttcctca aaagttttga gcgtgcgata
     2161 cagcgtgata cggtccgatt cgtgcaattg ctcttcgagg tctttttgcg aaagggcgcg
     2221 ttcgtaccgg aggaaaatat ccagcacgcg ctccctgaac tgggttttcc gcagttggtg
     2281 gttggagagc aattgaatcg aattcatacg cacggttagt tggtgatgcg ggcagaatat
     2341 atgatggggg gcgccgcatt gttgaccccg tcataagccc aaaccgtgac cacataatcg
     2401 ccattggccg ccgtattggg cacgggcaaa gttaaggaaa agtctttgta atcatcatca
     2461 gcggcagtaa aaaccaatgg gctgccccag cggaaagtat tgccggaggc gttggagcga
     2521 taaaaaattt ccactttgcc gttaccgccc tcagccagcg ggcggttgtc ggccacattg
     2581 ccgctgaaag tgatgttctg tcctttgcgc aaaacgagca tatcgccgga gttgacgatg
     2641 gggctgctga tgttgagggc aggtggcgtg gtgtcggcgg cgttgagcac tttgatcgca
     2701 taaacataag cagtaggatt gtcattgccg gctttgtcga gcacctgaat ttggaaatgg
     2761 nnnnnnnnnn nnnnnnnnca cattggcagg taccggcagc cggagttgga gcaggtattc
     2821 tttgccgctg aggttgccgg tttgcagcac caaccagtct tcggtatcca ttttgtggcc
     2881 atggcaatcg aaattggtgt gaatgtccac tttgtactgc gacaattcca cgtcgtcgct
     2941 caggcgcagg tccaaagaga gcgtatctcc gctgcgaaga tgaaacacga cctgctcttc
     3001 ggcgccacaa ataaaatcgg gcgtgggttc gggcgacagg gcgatgaaag ccaaagtagg
     3061 cgctgtatcg tccacatttt ttggcccgca ggacgataac aacccgataa aggctgccag
     3121 gatgagtaat gtatggctgt taattttcat ttttaaggtt ttccggttaa acaagagact
     3181 gcgaagtcaa cgtttttaag tgcaacattg ttgcgtgttg gttattcact cataccatgc
     3241 gactgtacat caccctcctt ttcctcctct acactctcct cgcgcaagcg caaatcagcg
     3301 gcaaggtagt ctccgaacgc ggcgaaaccc tgcccggtgc attggtgttg ctgctgcccg
     3361 actccgcttc gaccatcaca gacgctaatg ggagttttgc attttccaac ctgcgccccg
     3421 gtcgttacgc actggaagtg agctttttag gcttcaacat ctggcgcagc gaactgacgc
     3481 tggagcgaaa accgctgcgg gtagaggcgg tgttgggcag ccgggatcaa ctgctggaaa
     3541 cggtggtagt gagcgccgac catgccaaac aggagagcac gctttcgcac gagcacgttt
     3601 cgctcaattt catcacgcag cagcggggcg gcaccctggc ccaagccata gaaaaactgc
     3661 ccggactgag cgccatcacg gtgggtacgg gcattgccaa acccgttatt cgcggcctgt
     3721 cgttcaatcg catcattgtc aacagtcagg gtatcaagca ggaagggcag cagtggggcg
     3781 ccgatcacgg cctcgaactc gaccagtttg acgccgagca ggtggagatc atcaaagggc
     3841 cggcctcgct gcaatacggc tccgacgggc tgggcggcgt catcaacata ctgccgggcg
     3901 ccattccgca aaaaaactcc tggtcgggca gcctgatggg cttgtacaaa agcaacaacc
     3961 atcactgggc cggctcggct tttgcggccc tcaaccgcaa cgactggttt gccacagcgc
     4021 gtttcagccg gcaggactac ggcgcgtttc gagtgcctgc cgaccgcttt gtttataatg
     4081 gtttcgagtt gcccatactc gacaacacgc tgaccaatac cggcggtcgc gaaaataatt
     4141 tcaacctcaa tctggggcgc aaaacagcga acagcatcac gcgattcact tacagcctgt
     4201 acgatctgga agccggcttg ttccccggcg cggtgggcat tccgcgcagt tataccatcg
     4261 tacccggcct cgatccgcgt gctgtgagcg tgcccaaaca aaccgtctcg caccacaagg
     4321 catcgctgag ccggctgatg ttttggaatg aaaatcacgc cgaagtcaac atcggatacc
     4381 aatacaacct ccgccgggag ttctctttcc cggaaaatca caaccgcttc ctgctcagcg
     4441 atcccaacga ccggctggcc ctgcaactcg acctgcaaac ctggtcggcc aacggccaca
     4501 tccaattgca ggcctccgaa atctggaaaa ccacctatgg attcaacgcg caataccaga
     4561 ccaatcgccg ggccggattc gagtttttgc tgcccgattt cagctcgttc cgaaccggca
     4621 ttttcagcat tgtggaagcc gagcctactg atcgaatggt ctttaccggc ggtgtgcgtt
     4681 tggatttggc gggcaatgcc acgcaatttt tcgagcagcc gtttttcgac agccggggca
     4741 atgtgctggg ctatcagcgc gccgcagcca ccgatcagtt gttcttcaat gtgtccgctt
     4801 ccgcgggatt gaactacgag ctgattcctg agaacctgac catcaaggcc aatgccggca
     4861 agagtttcag ggcgccctac cccaacgaaa cttcttccga cggcgtgcac catggcaatt
     4921 ttcgccacga actcggcacg cctgatctgc gctccgaaca cggctatcaa ctcgacctcg
     4981 gcgccgaatg ggaaagcccg cattgggaag tggccgccgc cgcttatgtc aactatttcg
     5041 acaatttcat ttacctccag cccagcggtc gtttcgtttt tcggcccgat gccggccaga
     5101 cctaccagta tgtgcagcac gatgcggttt atacgggctt cgagttggac gccacctgga
     5161 agctgaatcg ggcctggacc ctgcacggaa atggcgaata tgtgtggaat ctgaatttgg
     5221 acgaacagcg agccttgcct tttacgccgc cgccctcggt gatgctggaa ctgcgcctgc
     5281 agccagcccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnncgcatt atttctttgc caaaacctca gccactgtag cccaaaacga ggcgaccacg
     5401 ccggcctaca acttggtgga ggcgggcatc agcggcagtc tgccctgggg caagcatcct
     5461 ccgcaatgga gcctgatggt gcaaaacttg ctcaatacgt cgtatttcaa tgcgctgagt
     5521 cgttatcgca tcctcaatat accggagcag gggcggaatg tggtgctgtc gnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn gctgcccaat gatgaccaat tctattttac atgccttcca acaggctcgc
     5761 ctcggcgggc aaaaacaaat tgccgtattg gtggatcccg acaaaaccag cccggattcg
     5821 gcggctgtat tggctcgcag ggcatcggag gcgggcattc attacctgat ggtgggtggc
     5881 agtttggtgg tggacgggca gtttgaactt tgcgtagagg ctctccgggt acattccga
//
LOCUS       DAORLF010000301         5927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7288_length_5927_cov_2.184605, whole genome shotgun sequence.
ACCESSION   DAORLF010000301 DAORLF010000000
VERSION     DAORLF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7288_length_5927_cov_2.184605"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PK198_19535"
     CDS             <1..1156
                     /locus_tag="PK198_19535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF40997.1"
                     /translation="DDADGICNFADLCPNFNNNLIGQACDDGDAYSSGETYFSDCTCR
                     GGAANLSYCAAEGSSGTGSDYIAQVTCNTLDHASGKTMYSDFRANSTEVAYGNSYQLT
                     VRLNYAFSPDRVHAWIDWNKNSVFDANELIVMTLPDANHRSIGSVSVPQGALPGATTM
                     RVRCVYSTTYNSPCGSEFGEVEDYTVFVRCTDPAGDCGLAVLPLQWERFDAWPLGTAD
                     ALLEWESSEEEELAHFEVERSLDGIHFRVMGQLPARNLPDRHTYRWIDRDVPAEQAYY
                     RIAAISFDGKAEYSTIRFVRWNKADRMSLKIHPNPVGNGETLHLNWSLKKNEGMSWYI
                     YNAHGQEVLHGKIADGSNRLIISIDGLPTGLYLIRLRASDGDSVLRFLRQ"
     gene            1182..>3919
                     /locus_tag="PK198_19540"
     CDS             1182..>3919
                     /locus_tag="PK198_19540"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRF40998.1"
                     /translation="MKPHLTLLLLAAAAIAQAQQSLRFLPDQADDTPPWGVWLYQEQP
                     NVAKVDSAYRAYYAGHDFVKTSHTQYYKKWRRAVEPFVQPDGSVLLPQNERRETWEQL
                     WLEQRAAAPKSNTTPWQHIGPVETFSTSPAQVKVSWQANVYCIDQSSANPDILFCGTE
                     AGGIYKTTNKGLQWTHVSANTTMRAIHSIKIDPANADVVYAGDGSRVYKTVDGGQNWS
                     VVFTSSGLNVNDISVNPGNPAILLVAADKGLYRSTDAGQNWTQLYTQACWDIEVHPTL
                     PDIVYVLKTNPAAGRCEFFKSLNSGATFSIRENGWDSSADAARRDDGARMTVTPADPN
                     VVYVVLAGNSKPGDNGFIGVFRSSNAGESWTLPNPPVGGPWTASHPNLMTLNNTNTLY
                     QGYYNLSIAASHTNADQVLIGGLNLWKTDNGAQTFTALGGYQGSLSWIHPDQQEIKIL
                     GNDCWVANDGGVEYSGNFFSSRESRKNGITASDFWGFGSGWNEDLLVGGRYHNGNSAT
                     RNTFPQGQFLRLGGGEAPTGYVNPGIAGKAYFSDINTRVIPTNIEEEVLSLPQMSDYP
                     GESYYAAHSSEMEFHPLCYRRVFIGKDNQLWRSEDEGASFQLVKTFDGSTEPVMHFEI
                     SRANPDVMYVYQRTSFYGATLWRTADGGQSWQARSFPSANSQRAGTMTLSATDPNTLW
                     VAFGHQNNDGVKVFKTTDGGQSWQNLSTPTLNGHSIQYIFHQAGSNGAVYAGTNFAVF
                     YRDDSMSDWEAYYEGLPASANCNIIRPFYKENKLRMATYSHGIWETDLKTPSATLAQP
                     MADKLTAYCARDTFYFDDYSVLRHAGAAWQWAFPGAAYVSDATARNPKVVFGQTGVFD
                     VSLTITDAAGQSSTKTVPGMIRIETDGCSPDTIPGEALRLGGATSDFAVGPALHTP"
     assembly_gap    3920..4049
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4050..5195
                     /locus_tag="PK198_19545"
     CDS             <4050..5195
                     /locus_tag="PK198_19545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF40999.1"
                     /translation="YHWNDGFWSWNSGLVVPDGEWAHIAMVVEPNRTTLYLNGKPAVN
                     NGNHAMQPFEAAFYLGADPNHSGRRFKGQMEEVCIWNRALSVNEIRKLRHLVKVPAED
                     PALLAYYQFNETTGDALDRSGLRHLRLSGAAVRTASSCPVGIGTSTRLAVNAAGDYFF
                     GNDTGVRMTLLSGGTVPNGEVYLTRINLPPDEPVNDAPHSRSYWIFNNYGTNPLITPP
                     TALTFSRIGPVEPAEVFQPQRFRLYVRSENADGETWQFSDPASELISGGDGGVYFGPE
                     TNVESLGQMIIARDGSPVSSAGEPERQPYEFAVYPNPASSGGGLTIATERSGDYRFTL
                     FDGAGRRVAETQGRGRAALSLPALPSGVYSWRIQMADYLEYGKLVVE"
     gene            complement(5527..>5927)
                     /locus_tag="PK198_19550"
     CDS             complement(5527..>5927)
                     /locus_tag="PK198_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF41000.1"
                     /translation="PPPPPPPPLPPPTPDDIFKIVDRMPCFPGCENEADLSACAKMKV
                     DDFFSKNMRYPALARENGVQGTVIVTFVVEKDGSMSDIKLVRDIGAGCGQEALRLVNL
                     MQEQGLRWTPGQHQGNTVRVQYNLPMMFKL"
BASE COUNT         1252 a         1773 c         1586 g         1186 t
ORIGIN      
        1 cgatgacgct gacgggattt gcaacttcgc cgacctctgc cccaatttca acaacaacct
       61 catcggccag gcttgcgatg atggggatgc ttactcatcg ggtgaaacat atttctccga
      121 ctgcacatgc cgaggcgggg ctgccaatct tagctattgt gccgccgagg ggagcagtgg
      181 caccggctca gactatattg ctcaggtaac atgcaacacg ctggatcacg cgtccggcaa
      241 gacaatgtat tcggacttca gggccaattc caccgaagtg gcgtatggca attcctatca
      301 acttacggtg cgcctgaact atgccttttc ccccgaccgc gtacatgcat ggattgactg
      361 gaacaagaac agcgttttcg atgccaatga actgattgtc atgacattgc cggatgccaa
      421 tcatcgcagc atcggctctg tgagtgttcc gcaaggcgcc ctgcccggcg ctaccaccat
      481 gcgggtgcgg tgtgtttatt ccaccactta taacagcccc tgtggttcgg aatttggcga
      541 agtggaagac tatacggtgt ttgtgcgctg caccgaccct gccggggatt gtggtttggc
      601 cgtcctgccc ttacaatggg agcgatttga cgcctggccg ctcggaactg ccgacgccct
      661 gctggaatgg gagtcgtcgg aagaagaaga actggcgcat tttgaggtag aacgctcttt
      721 agatggcatc cactttcgtg taatggggca gttgccggcg cgcaacctgc cggatcgcca
      781 tacctatcgc tggatagatc gcgatgtacc tgccgagcag gcttactaca gaatcgcggc
      841 gatttctttt gatggcaagg ctgaatattc aaccattcga ttcgttcgct ggaacaaagc
      901 agatcgaatg tcgctgaaaa tacatcctaa tccggtgggt aatggagaaa cgctgcacct
      961 gaactggtct ttgaaaaaga atgagggcat gagttggtac atttacaatg ctcatggtca
     1021 ggaagtgctt cacggcaaaa tcgcagacgg cagcaacagg ctgattatca gcatagatgg
     1081 tttgcccacg ggcctgtatc tgattcgcct ccgcgcttcg gatggagact cggtgttgcg
     1141 gtttttgagg caataagtag aacaccatca tttctaataa catgaaacca catctcaccc
     1201 tcctcctcct cgctgccgcc gccatcgcgc aggcacagca aagcctccga tttttgcccg
     1261 accaggccga cgacacgccg ccctggggcg tttggctcta tcaggagcag cccaacgtag
     1321 ccaaagtgga ctccgcctac cgggcctact acgccgggca tgattttgta aaaacctcgc
     1381 atacacagta ttataaaaaa tggcgccgcg cggtcgaacc ctttgtgcag cccgacggca
     1441 gcgtgctcct gccccaaaac gagcgccgcg aaacctggga acaactctgg ttggaacaac
     1501 gcgccgccgc tcccaaaagc aataccacgc cctggcaaca catcggtccg gtggaaacct
     1561 tcagtacttc gcccgctcag gtgaaggtct cctggcaggc caatgtctat tgcattgacc
     1621 agtcatctgc caaccccgac atcctgttct gcggcactga ggcgggaggc atttacaaaa
     1681 ccaccaacaa aggcttgcaa tggacgcacg tctcggccaa tacgaccatg cgcgccattc
     1741 acagcatcaa aatagaccct gccaacgccg atgtggtgta tgccggcgac ggatcgaggg
     1801 tgtataaaac cgtggacggc ggccaaaact ggtcggtggt ttttacctct tccggtctga
     1861 atgtcaacga catatcggtg aatccgggca acccggccat cctgctcgtg gctgccgaca
     1921 aaggcctata ccgcagtacc gacgcaggcc aaaactggac gcaactctac acccaagcct
     1981 gctgggacat tgaggtgcat cccaccctgc ccgacatcgt ttatgtactg aaaaccaacc
     2041 cggcagccgg gcgctgcgag tttttcaaat ccctcaattc cggcgctact ttcagcatcc
     2101 gcgagaacgg ctgggacagt tctgccgacg ctgcccgccg cgacgacggc gcccgcatga
     2161 ccgtcacgcc cgccgatccc aacgtcgttt atgtggtgct ggccggcaat tccaaacccg
     2221 gcgacaacgg cttcatcggc gtatttcgga gcagcaatgc cggcgaaagc tggaccctgc
     2281 ccaacccgcc cgtcggtggc ccctggacgg cctcgcaccc caacctgatg accctcaaca
     2341 ataccaacac cctctatcag ggctattaca acctctccat cgcagcctcg cataccaatg
     2401 cagatcaggt gctcatcggc ggcctcaacc tctggaaaac cgacaacggc gcacagactt
     2461 tcacggccct cggcggctat cagggcagcc tcagttggat tcaccccgac cagcaggaaa
     2521 tcaaaatctt aggcaacgat tgttgggtgg ccaatgacgg cggcgtggag tattccggca
     2581 actttttcag ctcccgcgaa tcgcgcaaaa acggcatcac cgcctccgat ttctggggct
     2641 tcggcagcgg ctggaacgaa gacctgctcg taggcgggcg ctatcacaac ggcaattctg
     2701 ctacccgcaa caccttcccg caagggcaat tcctgcgcct cggcggcgga gaagcgccca
     2761 ccggctacgt caatccgggc attgccggca aggcttattt ttcagacatc aacacccgcg
     2821 tcattcccac caacattgaa gaagaagtgc tgagcttgcc gcaaatgagc gactaccccg
     2881 gcgaatcgta ctacgcggcg cactccagcg aaatggagtt tcatccgctt tgctaccggc
     2941 gggttttcat cggcaaagac aaccaattgt ggcgctccga agacgagggc gcgagttttc
     3001 aattggtcaa aacttttgat ggaagcactg aacccgtgat gcacttcgag atttcgcgcg
     3061 ccaaccccga cgtgatgtac gtctatcagc gtacctcgtt ttacggtgct accctgtgga
     3121 gaactgccga tggcggccaa agctggcaag cccggagctt cccctccgcc aactcgcaac
     3181 gcgccggcac catgaccctc agcgccaccg atcccaacac gctttgggtg gctttcgggc
     3241 atcagaacaa cgacggcgtc aaggttttta aaaccaccga cggcggccaa agctggcaaa
     3301 acctgtccac gcccacgctc aatggccatt ccatacagta catttttcat caggcgggca
     3361 gcaacggcgc ggtgtatgcg ggcaccaatt tcgctgtctt ctaccgcgac gactcgatga
     3421 gcgactggga ggcgtactac gaagggctgc cggcttccgc caattgcaac atcatcaggc
     3481 cgttttacaa agaaaacaaa ctccgcatgg ccacctacag ccacggcatc tgggaaaccg
     3541 acctgaaaac gccctccgca acgctggcac agccgatggc cgacaaactc accgcctact
     3601 gcgcccgcga tactttttat tttgacgact actcggtgtt gcggcacgcc ggcgctgcct
     3661 ggcaatgggc ctttcccggc gctgcttatg tgagcgatgc cacggcccgc aaccctaaag
     3721 tggtattcgg ccaaaccggc gtgttcgacg tctcgctgac catcaccgac gcggccggcc
     3781 agagcagtac caaaaccgtg cccggcatga tccgcattga aaccgacggc tgctcgcccg
     3841 acaccattcc cggagaggcc ctgcggctgg gtggcgctac ctccgatttt gcggtcggcc
     3901 cggccctcca taccccgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt accactggaa cgacggtttt tggagttgga
     4081 attccggcct cgtagtaccc gacggcgagt gggcgcacat tgcgatggtg gtagaaccca
     4141 atcgtactac gctgtatctc aacgggaaac cggctgtcaa caacggcaat cacgccatgc
     4201 agcccttcga ggcagccttt tacctcggcg ccgatcccaa ccattccggg cggcgtttta
     4261 aaggccagat ggaggaggtt tgtatctgga atcgcgccct ttcggtgaat gaaatccgca
     4321 aactgcgcca cttggtgaaa gtacccgccg aagacccggc actgctggcc tactaccagt
     4381 tcaacgaaac caccggcgac gccttagacc gttcggggct gcggcatctg cgattgagcg
     4441 gcgccgctgt gcgcacggct tcttcctgtc cggtgggcat cggcacgtcc acccgcctgg
     4501 ccgtcaacgc tgcgggcgac tactttttcg gcaacgacac cggtgtgcgc atgaccctgc
     4561 tcagcggcgg cactgtaccc aacggcgagg tgtatctcac ccgcatcaat ctgccacccg
     4621 acgagccggt caacgacgcg ccacattccc gctcgtattg gattttcaac aattatggca
     4681 cgaatccgct catcacaccg cccactgcgc tgaccttttc gcgcatcggg ccggtagaac
     4741 ctgccgaagt atttcaaccc caaagattca ggctgtacgt ccgctctgaa aatgccgacg
     4801 gcgagacctg gcagttcagc gacccggcat ccgaactcat cagcggcggc gacggcggcg
     4861 tatatttcgg cccggaaacc aatgtagaaa gtctgggcca gatgatcatt gcccgcgacg
     4921 gttcgcccgt cagcagcgca ggagagccgg aacgccagcc ttatgagttt gcggtgtatc
     4981 ccaatccggc gagttccggc ggcggtctca ccatcgctac cgagcggagc ggcgactacc
     5041 gtttcacttt gttcgacggg gcaggccgcc gggtagcgga aacgcagggg cgtggccgtg
     5101 cagcgctgtc attgccggca ttgccttcgg gcgtgtattc ctggcgcatc caaatggctg
     5161 attatttaga gtatgggaag ttggtggtgg agtagggttc gggagggaac ggctgccggc
     5221 tgttagctgc tggctgcaag ccacgcaggg cgtacaccct cgcgctgcgc cacgacggag
     5281 gcgtggttcg cggaaattcg cgaccttcgc taaaattcgc gttaaaaatt cgcgttccgg
     5341 gcctcgccac gacgaaggcg tggttcaaag tttcgggttc aaggcttcgg gtttcatccg
     5401 ccgtagcctt ggcaaaggcg ggtttcgcgt tcagagggta gtaaagggaa gacttagcat
     5461 gtttcatgcc ctgtagtcat tccttcgcta agctggcgac gccacctcgc ccatcacatc
     5521 cccgcctcac aacttgaaca tcataggtag attgtactgc acacgcaccg tattgccctg
     5581 atgttggccg ggtgtccagc gcaggccctg ctcttgcatg aggttgacaa ggcgcaaggc
     5641 ttcctgcccg catcctgcgc caatgtctcg gactaatttg atgtcagaca ttgagccgtc
     5701 tttttccaca acaaatgtga caatgaccgt gccttgcacg ccgttttcgc gggccagagc
     5761 ggggtatctc atgtttttgc tgaagaagtc atcaactttc atctttgcac aggcagagag
     5821 gtcggcttca ttttcgcagc cgggaaagca gggcattcga tctactattt tgaaaatatc
     5881 gtcaggagtg ggcggcggca gtggcggtgg cggcggcggt ggcgggg
//
LOCUS       DAORLF010000302         5919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7300_length_5919_cov_1.674625, whole genome shotgun sequence.
ACCESSION   DAORLF010000302 DAORLF010000000
VERSION     DAORLF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_5919_cov_1.674625"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..>1094
                     /locus_tag="PK198_19555"
     CDS             222..>1094
                     /locus_tag="PK198_19555"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41001.1"
                     /translation="MKQYLQHHEWHIIEEGFQPEHNRISESVCSIGNGRMGQRANFEE
                     RYSGDTLPGNYVAGIYYPDKTRVGWWKNGYPEYFAKVLNAPNWIGIDIAFDGEQLDLA
                     QCKVESFRRLLDMQTGRLERYVTAEMADGRKVEIASVRFCSIADDETGAIRYSVKPLN
                     FSGTLTITPYVDADVINQDSNYGEKFWVEVETKTKRSQAYITAETKKTEFQVCTGMRF
                     SIFQNGAKIEFDSERVHKEKYAGRSVNVKCEKGKETVIYKYAAVVSSLNYAKEELLER
                     CQDAVKRAYDTGFEA"
     assembly_gap    1095..1111
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1112..>1462
                     /locus_tag="PK198_19560"
     CDS             <1112..>1462
                     /locus_tag="PK198_19560"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRF41002.1"
                     /translation="AQADAWASKWEEADIRIRGDVAAQQGIRFNIFQLYQTYTGEDAR
                     LNIGPKGFTGEKYGGSTYWDTEAYCFPFYLATADQHVARNLLVYRYKHLQKAIENAEK
                     LGFTHGAALYPMVT"
     assembly_gap    1463..1497
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1498..>2414
                     /locus_tag="PK198_19565"
     CDS             <1498..>2414
                     /locus_tag="PK198_19565"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRF41003.1"
                     /translation="NEWEITFEEIHRNGAIAYAVYDYVRYTGEKEYLAEYGAELLLGI
                     ARFWSQRVHFSKPKGQYVMHGVTGPNEYENNVNNNWYTNYIAAWCLRYAGEALDYVKE
                     NAPERYRDLIAKTAFDEAAERTRWADIAGNMFLPKDEQLGIFLQQEGFLDKDVMTVDQ
                     IPAGQRPINQNWSWDRILRSCFIKQADVLQGLYFFEDHFSEDEIRRNFDFYEPLTVHE
                     SSLSPCVHVILGARLRKEEKAYEMYLRTARLDLDDYNNDTEDGCHITSMAGTWMSFVK
                     GFGGMRVYDDTLHFSPMIPKQWTDYAFKIR"
     assembly_gap    2415..2438
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2581..3363
                     /locus_tag="PK198_19570"
     CDS             2581..3363
                     /locus_tag="PK198_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRF41004.1"
                     /translation="MSAGYNIQIPQFQGPFDLLLFFIERDELDIQDIPIAKITNDFLD
                     YIHTMERLNIDLAGEFMLVAATLCKIKARMLLPRKQVDEQGNEIDPRQELVDRLMEYK
                     RYKSVLDEMRALEEERAQKTTRGNLSDELQQIATRALVDSELETLTLFRLLKAFERVM
                     QRMENAQPRAIHQIAKFSYTVEEQQIFILSKIPQGGRADFQTVFSTCANRIHAIVTFL
                     ALLELLNMQRVLILAGSGVNSFWLEAVEEDTPTVADNHIEAG"
     assembly_gap    3565..3591
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3629..>3969
                     /locus_tag="PK198_19575"
     CDS             3629..>3969
                     /locus_tag="PK198_19575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF41005.1"
                     /translation="MAEIFIQKAIEEEQINPLGIKYQVADAQLLPYETGQFDFATAFM
                     SLMDVPEPETAIQEAFRVLKPGGFLQFSISHPCFSTPHRRNLRNAQGETYAIEVGGYF
                     QNVNGEWIEEW"
     assembly_gap    3970..4055
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4229..4792)
                     /locus_tag="PK198_19580"
     CDS             complement(4229..4792)
                     /locus_tag="PK198_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF41006.1"
                     /translation="MSFEIIKSPVVSLPVQHSSIPEQLEKITQTLRAFALKLTGNEMD
                     AEDLYQDTTMRIITNSEKYLQGTNFKAWAVTIMRNIFINNYRKRVRRNLIIDQTPNNY
                     YINSGDNSIGNEGESDITFIELLGMVNTLPEDFRKPFMMAYEGYKYEEIAEELGSPLG
                     TIKSRIFFARKKLQKMYADFYMERERA"
     assembly_gap    4938..4973
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5023..5547
                     /locus_tag="PK198_19585"
     CDS             5023..5547
                     /locus_tag="PK198_19585"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF41007.1"
                     /translation="MTVQQQWCNTHNDLHPAMYLHQNIRALRKKLNLSQEELALRVGL
                     NRGNIASYENGTAEPKICNLLKLSKIFGVSVIDLTNRDFSAEAIGWAAEPAEVVVQPL
                     HDPDQLLQLRDRAAEISQVINSLYTCFQFKLKSMTECAPEIQALQVHFEQMKEVSQSL
                     INDHLALLDIMDRR"
     gene            5642..>5919
                     /locus_tag="PK198_19590"
     CDS             5642..>5919
                     /locus_tag="PK198_19590"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF41008.1"
                     /translation="MPRSTPLLAWTALFVLFTMTLHAEVQFVVKPLPLVKDMAARQGK
                     LYFVHFTAQWCMPCRWMDENTFSDPALSEFVQQHYIAVKMDIDDTYGL"
BASE COUNT         1506 a         1533 c         1375 g         1280 t
ORIGIN      
        1 ggggttttga gatggacggc ttgttgagtg tgttcaagag ccacaagcca ttaccatgcg
       61 gggcgtggtt gtttttatgt tcagaaatca gattttcctt cgggggaatt tctaaatgta
      121 cttttgaaaa tgattttttt tattccttcg gaaaccaagc ttgcagcctg tagcctgtag
      181 cttgcagccc aagaggaact atcaataaat acctgcaact aatgaaacaa tacctgcaac
      241 accacgagtg gcacatcatc gaagaagggt ttcagcccga acacaaccgg atttctgaaa
      301 gcgtgtgcag catcggcaac ggccgcatgg ggcagcgcgc caattttgaa gaaagataca
      361 gcggcgatac cctgcccgga aactatgtgg ccggcattta ttaccccgat aaaacccgcg
      421 tgggctggtg gaaaaacggc tatcccgaat acttcgccaa agtgctgaac gcccccaatt
      481 ggatcggcat agacattgcc ttcgacggcg aacaactcga cttggcacaa tgcaaggtag
      541 aatccttccg ccgcttgctc gacatgcaaa ccggccgctt agagcgctac gtcaccgccg
      601 aaatggccga tggtcgcaag gtggaaattg cctccgtccg cttttgcagc atcgccgacg
      661 acgaaaccgg cgccattcgt tacagcgtca agccgctcaa cttctccggc acgctcacca
      721 tcaccccgta tgtggatgcc gacgtcatca atcaggactc gaactacggc gagaaattct
      781 gggtggaagt agaaaccaaa accaagcgca gtcaggcata catcaccgcc gagacgaaaa
      841 aaaccgagtt tcaggtgtgt accggcatga ggttttccat tttccaaaac ggtgccaaaa
      901 tcgagttcga ctccgagcgc gtacacaaag aaaaatacgc cggtcgcagc gtgaatgtga
      961 agtgcgaaaa gggcaaagag accgtgattt acaaatatgc ggcggtggtt tcttcgctca
     1021 attacgccaa agaagaactc ctcgaacgct gccaggatgc cgtcaaacgc gcctacgaca
     1081 ccggtttcga ggccnnnnnn nnnnnnnnnn nggcgcaagc cgatgcctgg gcttccaaat
     1141 gggaggaagc cgacatccgc attcgcggcg atgtagctgc acagcagggc attcgtttca
     1201 atattttcca actctaccaa acctacaccg gcgaagatgc ccgcctcaac atcggcccca
     1261 aaggcttcac cggcgagaaa tacggcggca gcacttattg ggacaccgag gcgtattgct
     1321 tcccgtttta cttggccaca gccgatcagc atgttgcacg caatttgctg gtatatcgct
     1381 acaaacacct gcaaaaagcc atcgaaaacg ccgaaaaact tggcttcact catggcgcgg
     1441 ccctctaccc catggtcacc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac
     1501 gaatgggaaa tcacctttga agaaattcac cgcaacggcg ccattgccta cgccgtatat
     1561 gactacgttc gctacaccgg cgaaaaggag taccttgcgg aatacggcgc agaattgctg
     1621 ctcggcatcg cccgtttctg gtcgcagcgc gtacacttct ccaagcccaa aggccaatac
     1681 gtgatgcacg gcgtcaccgg ccccaacgag tacgaaaaca acgtcaacaa caactggtac
     1741 accaactaca tcgccgcctg gtgcctgcgc tacgccggcg aagcgctcga ttatgtgaag
     1801 gaaaacgcgc ccgaacgcta ccgggacttg atcgcaaaaa cggcctttga tgaagccgcc
     1861 gaacgcaccc gctgggccga tattgccggc aatatgttcc tgcccaaaga cgaacaactc
     1921 ggcattttcc tccagcagga aggcttcttg gacaaagacg ttatgacggt agatcagatt
     1981 cccgccgggc agcgccccat caaccaaaac tggtcctggg atcgcatcct ccgctcttgt
     2041 ttcatcaagc aggccgacgt actgcaaggc ttgtacttct ttgaagacca cttcagcgaa
     2101 gatgaaatcc gccgcaattt cgacttctac gaaccgctca ccgtacacga atcctccctc
     2161 tcgccctgcg tacacgtcat tctcggcgcc cgcttgcgca aagaggaaaa ggcctacgaa
     2221 atgtacctcc gtaccgcccg cttggacctc gacgactaca acaacgacac cgaagacggc
     2281 tgccacatca ccagcatggc cggcacttgg atgtccttcg tcaaaggctt cggcggcatg
     2341 cgcgtatatg acgacacgct gcatttcagc ccgatgattc ccaaacaatg gaccgattac
     2401 gcgttcaaaa tccgnnnnnn nnnnnnnnnn nnnnnnnngc gctttgcttc aggtaaaagt
     2461 cactcaggaa caggccattg tgcgcaatct atcggcccaa agcctgcctg taaaagtgta
     2521 tgggaaagaa caccatctgg aggccggcga ggaactgagc gtggggatta aatagccatc
     2581 atgtccgccg gatacaacat acaaatccct caatttcagg ggcctttcga tctgctgctg
     2641 tttttcatag agcgggacga gctggatatt caggatattc ccattgccaa aatcaccaat
     2701 gacttcctgg attacatcca caccatggag cgcctcaata ttgaccttgc cggcgaattt
     2761 atgctcgtag ctgccacctt gtgcaagatc aaagcgcgca tgctcctgcc ccgcaaacag
     2821 gtggacgaac agggcaacga aatagacccc cggcaggaat tggtggaccg cctcatggaa
     2881 tacaagcgct acaaaagcgt gttggacgaa atgcgggcgc tggaagaaga acgggcacaa
     2941 aaaaccaccc gtggcaatct gagcgacgaa ctgcaacaaa ttgccacccg cgctttggtg
     3001 gattccgagt tggaaaccct caccctcttc agactcctca aagccttcga gcgggtgatg
     3061 caacgcatgg aaaacgcaca gccgcgcgcc atccaccaga ttgccaagtt cagctatacc
     3121 gttgaagaac agcagatttt catcctctcc aaaataccgc agggcggtcg tgccgacttc
     3181 caaacggtct tcagcacttg cgccaatcgc atccatgcca tcgttacatt tctcgctttg
     3241 ttggaattgc tgaacatgca gcgcgtgctc attctggcag gttccggggt gaatagtttt
     3301 tggctggagg cggtggagga ggacacgcca acagtcgccg ataaccatat cgaagctgga
     3361 taatcttgcc accaacaaca actgcatcct atatgaaccg agaagaagcc gcccgtttat
     3421 ggaataataa tgccgaagcc tggacggttt tggcaagagc gggctatgat ctttaccggg
     3481 accatctcaa tacgcctggc tttttccaaa tcctgccgga tgtcaatggc ttatatggtt
     3541 tggacatagg ctgcggcgaa ggctnnnnnn nnnnnnnnnn nnnnnnnnnn ngttggctgc
     3601 ccgaggcgct cgaatggatg cgatagatgt ggctgagatt tttatccaga aagccataga
     3661 agaggaacaa atcaacccgt tgggaataaa gtatcaggta gctgatgcgc aactattgcc
     3721 ttatgagaca ggccaatttg atttcgccac cgcgtttatg agtctgatgg atgtgccgga
     3781 gccggaaacc gccattcagg aagcattcag agtgctgaaa ccgggaggtt ttttgcagtt
     3841 ttccatctcg cacccctgct tcagcacgcc gcaccgccgc aatttgcgca acgcccaggg
     3901 cgaaacctac gccatagaag taggcggcta ttttcaaaac gtcaacgggg aatggatcga
     3961 agaatggatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgga tgagttacct gctggatgcg
     4081 ggttttgtca ttgagcaagt ccacgagcca tgtccagacg atgaaacgat tcggcaatat
     4141 ccgtatcttc aggacagctc ggtgacggcg tacttcctgc aaattcgttg cagaaaaaac
     4201 ctttgataat caataaattg aaggaaaatc aagcccgttc gcgctccatg taaaaatcgg
     4261 cgtacatctt ttggagcttc ttgcgcgcaa agaaaatgcg gcttttgatg gtaccgagag
     4321 gcgaacctaa ttcttcggca atttcctcat atttgtagcc ttcgtaggcc atcataaacg
     4381 gcttgcggaa gtcctccggc aaggtattga ccatgcccag caactcgatg aaagtgatgt
     4441 cggattcacc ttcgttgccg atgctgttgt cgcccgaatt gatgtagtaa ttgttcggag
     4501 tttggtctat gatgaggttg cgacgaacgc gcttgcgata gttgttgatg aaaatattgc
     4561 gcatgatggt caccgcccaa gccttgaagt tggtaccctg aagatacttt tcagagttgg
     4621 tgatgatgcg catcgtggta tcctgataca ggtcttccgc atccatttcg ttgcccgtga
     4681 gtttgagcgc gaaagcccgg agcgtttggg tgatcttctc caattgctca ggaatactgc
     4741 tatgttgaac aggcaaagac acgacgggcg actttatgat ttcgaaagac attttgtaat
     4801 atttgtttac aaaattagaa actaaacgtg agttctgcaa aaaatttttt ctatttagac
     4861 tgaatttcga cgaaaaagta gcagaaatga caactccgag aaaaaacgtt aaaaaaatta
     4921 actaaaataa accttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagaatta
     4981 tttatggaac gatattgagt ttctgtcgaa aacttccaac acatgaccgt acaacaacaa
     5041 tggtgcaata cgcacaatga ccttcacccg gccatgtatc tccatcagaa catccgggca
     5101 ctgaggaaaa aactcaacct cagccaggaa gaactcgccc tgcgcgtagg cctcaaccgg
     5161 ggcaatatcg cctcttatga gaacggcacc gccgaaccca aaatctgcaa cctcctcaag
     5221 ctctctaaaa tcttcggcgt ctccgtcatt gatttaacca accgggactt cagcgctgaa
     5281 gccatcgggt gggctgccga gccggcagag gtagtagtcc agcctcttca cgaccccgat
     5341 cagttgctgc aactccgcga ccgggccgcc gaaatctcac aggttatcaa cagcttatac
     5401 acctgcttcc agttcaaact caagtccatg accgagtgcg cccccgaaat tcaggcgctt
     5461 caggtacatt ttgagcaaat gaaggaggtt tcgcaatcgc ttatcaacga tcacctcgca
     5521 ttgttggaca ttatggaccg ccgatagaaa aaaataatac ccgccccaac atattttagc
     5581 atttcccgta tatgtatttt gtagaatgct ttagatttgt aatcccttaa acattacaga
     5641 aatgcctcgt agcacccccc ttctcgcctg gacggcctta tttgtgctgt tcaccatgac
     5701 cttgcacgct gaggttcaat tcgttgtcaa gccgctgccc ttggtcaaag acatggcggc
     5761 ccgacaggga aaactctatt ttgttcattt cacggcccaa tggtgtatgc cttgccgctg
     5821 gatggacgaa aacacttttt ccgatccggc gctgtctgag ttcgttcaac agcattacat
     5881 cgctgtaaaa atggacattg acgataccta cggtttaaa
//
LOCUS       DAORLF010000303         5905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7319_length_5905_cov_2.214530, whole genome shotgun sequence.
ACCESSION   DAORLF010000303 DAORLF010000000
VERSION     DAORLF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7319_length_5905_cov_2.214530"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1820
                     /locus_tag="PK198_19595"
     CDS             114..>1820
                     /locus_tag="PK198_19595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF41009.1"
                     /translation="MTTTTFAELGLSEAVLASVTDMGFINATPIQAEAIPVLMTGKDI
                     IGQAQTGTGKTAAFGIPLLERMTPDKYVQGLILCPTRELCIQVADEMKKLSTHSKGVQ
                     VVAIYGGDSYERQIKALKGGANIVVGTPGRVIDHIGRNTLKLENLQMVIMDEADEMLN
                     MGFREDMENILSGTPEERQTVLFSATMSKDILALTKKFQRDAQLIKVTREELTNSNTS
                     QYYLDVKHGAKLEALCRLMDVQQLELALVFCNQKRMVDEVAEKLRDRGYPAEALHGDL
                     PQAARNVVMNRFRNGSLNLLVATDVAARGIDVDDVDAVINFDMPYDPEVYVHRIGRTG
                     RAGRTGKAFSLVSGSDRRRLWDISRYTKVEVERMTVPSFQDVVRVRRQQFTQRVLTTV
                     EAGGLEPFEGLLPKLQEMGMSTEQIVAALVKMNFGELKNEFVQENIDATAERKAAPKR
                     EAFGKKAEFKPASKNKGGYAERNTPRSERFDKKSGKPYPSARGGSMTRLVINIGKQEK
                     ISPSNIVGAIAGESGIPGSVLGQIDIFDRYTSVDVPATLVDEVLRGMSGAKIKGKKGA
                     SSF"
     assembly_gap    1821..1830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1986..3059)
                     /locus_tag="PK198_19600"
     CDS             complement(1986..3059)
                     /locus_tag="PK198_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF41010.1"
                     /translation="MALNRILLFGLLAIFSSCNGQEKTNTLKGPNLSIPGPTVIRNFE
                     AVPLAPDFDTTLVSQYIRSIFQDSRGNLWFGTIGQGVVKYDGKALIYYSDPDGFINKT
                     VYAINEDKNGNLWFGTDQGVYKYDGKTFKNYASKEGLHHVDITRKGILVDKTGAVWVG
                     THGGVYRYDPVADGKGQPCFSLFQQLPPVNVAGIMEDKKGNIWFASSENGVFRYDGKT
                     MVNIVEKENLGENYAGGIAEDKAGNMWFTMKNGICKYDGKTLTEYTPKDGLGGIEFWG
                     IYIEESGIIWVTARGSTTRFDPSISLPNAAAFTVFTPAEGLNCCVQSMYQDRSGNMWW
                     GTGQGLYRFDGQRFYQVKQNGPW"
     gene            complement(3104..3811)
                     /gene="mqnB"
                     /locus_tag="PK198_19605"
     CDS             complement(3104..3811)
                     /gene="mqnB"
                     /locus_tag="PK198_19605"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRF41011.1"
                     /translation="MKLLIVAATPFEILPLEQHLKNAFVPHGQSQFQYGRLNVTLLVT
                     GVGLTATAFAMGQILAAQRYDLIINAGICGAYDRSLALGDVVQVVSEVFGDMGAEESD
                     GSFTDAFGIGLLGLDQPPFKNGLLLNEGGSAYDFLPKVAGVSVNKVHGSQASITALQQ
                     RLGAQVESMEGAAFFYACLMTQAPFLQIRAISNYVEPRNKDNWNIPLAITNLNDVLVA
                     IDRLNEVLAQMVNGLAV"
     gene            complement(3808..4641)
                     /locus_tag="PK198_19610"
     CDS             complement(3808..4641)
                     /locus_tag="PK198_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41012.1"
                     /translation="MKSNLLFLFNSITALAFCAALQAQTGIAPAAGARGIGMGNTGVV
                     FTDVHSALSNQAGLGMLDRSAALVFGEQRFLLADLRSLGAAYAMPVSGGAFAVSAQYF
                     GMKEYNEQRFGLAYGRQIFPGTSIGAQINVFTTRIPDYGNTLTLNAEIGLQTRLLPKL
                     LLGLHVANPVQTVTRGGDRLPTLLRTGLAYESSDKLLVVFELEKDIDFPLRAKTGFED
                     RPHEQVFLRLGASTQPASASLGAGYTLPGGLAFDFAAAWHSYLGITPAFGLTYSKPIK
                     K"
     gene            complement(4803..5528)
                     /locus_tag="PK198_19615"
     CDS             complement(4803..5528)
                     /locus_tag="PK198_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41013.1"
                     /translation="MKNMFVKNTIMMTFALFFGALALTACEKDQSVITPDEVAVIIEG
                     ALMTDSEGAAAGISEVVSIAEKFTLDAPSLSCGQTADTTFSRSLSNTRITADYTASAN
                     WTLQCNQFNVPQRIVMNLQSSGTYTTPRVSSNDNGTGSLTVSNLVTGPNFLINGSCTR
                     NGSQTRLLRNGNGATSTVAITLTNVAVSKTTRKIQSGSAEVLITGQGSDGQTINSTGS
                     IVFNGDGTATLSINGRTYTITLG"
     gene            5518..5709
                     /locus_tag="PK198_19620"
     CDS             5518..5709
                     /locus_tag="PK198_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41014.1"
                     /translation="MFFILVKDFGDELIDGAKMPTRNRGLQIVSTNGAKSSMTCLKED
                     IWPRSGCFFTVGRLFLTGR"
BASE COUNT         1418 a         1544 c         1589 g         1344 t
ORIGIN      
        1 aatttttctt cttcaacagg tatgggcgcg cgttaacgga tgattgaaag catctgttaa
       61 ccgggttttg tgagcttcct accgaagcct caacgctcac atcgtgtact tacatgacca
      121 caaccacttt tgctgaactc ggcctctcgg aggcagtgct cgcttccgtc accgacatgg
      181 gctttatcaa cgctacgccc attcaggcag aagccattcc tgtattgatg accggcaaag
      241 acatcatcgg gcaggcacaa accggcaccg gaaaaactgc cgctttcggc attccgctgc
      301 tcgaacgcat gacgcccgac aagtatgtgc agggcctcat cctctgtcct acccgcgagc
      361 tgtgtattca ggtagccgac gaaatgaaaa aactttccac ccactccaaa ggcgtacagg
      421 tggttgccat ttatggcggc gactcttacg aacgccagat caaagccctc aaaggcgggg
      481 ccaacatcgt ggtgggcacg cccggcagag ttattgacca tatcggcagg aataccctca
      541 aattggaaaa cctccaaatg gtcattatgg acgaagcgga cgaaatgctc aatatgggct
      601 tccgggaaga catggaaaac atcctcagcg gcacgcccga agaacgccag acggtgctct
      661 tctcggccac catgtcgaag gatattctgg ccctcaccaa gaaattccag cgcgatgccc
      721 aactcattaa agtcacccgc gaagaactca ccaacagcaa tacttctcag tattatttgg
      781 atgtcaaaca cggcgccaaa ctcgaagcgc tctgccgcct gatggatgtg cagcaactcg
      841 aactggcact cgtgttctgc aaccagaaac gcatggtgga cgaagtagcc gaaaaactgc
      901 gcgaccgcgg ctatcctgcc gaagcgctgc acggcgacct accgcaagcc gctcgcaacg
      961 tggtgatgaa ccgttttcgc aacggcagcc tcaacctgct ggtagctacc gacgtggccg
     1021 cccgcggtat tgatgtggat gacgtggatg ccgttatcaa cttcgatatg ccttacgacc
     1081 ccgaagtata tgtacaccgc atcggtcgca ccggccgcgc aggccgcacc ggcaaggctt
     1141 tctctttagt gtctggctct gatcgccgcc gcctgtggga catcagccgc tacacaaaag
     1201 tagaggtgga gcgcatgacg gtgcccagct ttcaggatgt agtgcgcgtt cgccgccagc
     1261 agttcacaca gcgcgtactg actaccgtgg aagccggcgg attagagccg tttgaaggat
     1321 tgctgcccaa actgcaggaa atgggcatga gcaccgaaca aattgtggcc gcattggtca
     1381 aaatgaactt cggcgaactg aaaaacgagt ttgttcagga aaatatagac gctaccgcag
     1441 aacgcaaggc tgctcccaag cgcgaggctt tcggcaaaaa agcggagttc aaaccagcca
     1501 gcaaaaacaa gggcggctac gcagagcgca atacgccgcg cagcgaacgc tttgataaaa
     1561 aatcgggcaa gccgtatcct tccgcccgtg gcggcagcat gacccgcttg gtcatcaaca
     1621 tcggcaaaca agagaaaatc agcccctcga acatcgtggg tgccatagcc ggcgagtcgg
     1681 gcattccggg cagcgtgctg gggcaaattg atattttcga ccgctacacc agcgtggacg
     1741 taccggctac ccttgtggac gaggtactgc gcggtatgtc gggcgccaaa atcaaaggca
     1801 aaaagggcgc atcgagcttc nnnnnnnnnn aaaagtacgg gtcagcgtgg agaattaatt
     1861 cattgcgttt gatctgaaat aaaaaaggcg ccttcccgat gcaatggggg gcgtcttttt
     1921 tttgcttgtg gtttggttac cactacattg tagaagcggc acgccacaag atgcttgata
     1981 tcatatcacc aggggccgtt ctgttttacc tgataaaacc gctggccgtc aaaccgataa
     2041 agaccttgcc ccgtacccca ccacatgttt ccagacctgt cctgatacat gctctgtaca
     2101 cagcagttca gcccctcagc aggcgtaaac acagtgaaag cggctgcgtt cggcaaggaa
     2161 atagaaggat caaacctcgt agtacttcct cgcgctgtga cccagataat acccgactcc
     2221 tctatgtata tgccccaaaa ttcgattccg cccaatccgt ctttgggcgt gtattccgtc
     2281 aatgttttcc catcatattt gcaaataccg tttttcatgg taaaccacat attcccagct
     2341 ttatcctccg cgatgccgcc ggcgtagttc tcgcccagat tttctttttc tacgatattg
     2401 accattgttt ttccatcata acgaaagact ccattttcag aagaagcaaa ccagatgttt
     2461 ccttttttgt cttccatgat gccggccacg tttacaggtg gaagctgttg aaataatgaa
     2521 aaacatggct gccccttgcc atcggcaacc ggatcatacc ggtaaacgcc cccgtgagta
     2581 cccacccaaa cagcacccgt tttgtcaacc aaaatgcctt tccgggttat atcaacatgg
     2641 tgaagcccct cctttgaagc ataattttta aaggtttttc catcatattt atacacgccc
     2701 tgatccgttc caaaccaaag gttgccattt ttatcttcat taatggcata caccgttttg
     2761 ttgataaaac catcaggatc agaatagtag attaacgcct ttccgtcata cttcactacg
     2821 ccttgtccaa tggtgccgaa ccatagattc cccctcgaat cctgaaaaat actccggata
     2881 tattgactga ctaaagtagt gtcaaaatcg ggagccaaag gaactgcctc gaagtttctt
     2941 atgactgttg ggcctggaat tgaaaggttc gggcctttaa gtgtgtttgt tttttcttgt
     3001 ccgttgcagg aggagaaaat tgccaaaagg ccaaagagca atatcctgtt taaagccatg
     3061 ccgataattt ttgaaaaaaa gatgttgttc agttgcttgc gactcacacc gccagcccgt
     3121 tcaccatttg cgccaacacc tcgttcaggc ggtcaatggc caccagcacg tcattaagat
     3181 tggtgatagc taacgggata ttccagttgt ctttgttgcg cggctccaca tagttggaaa
     3241 tggcgcggat ttgcaaaaac ggagcctggg tcatcaggca ggcgtagaaa aaagcggcgc
     3301 cttccatgct ttccacctga gcgccgaggc gttgttgcag cgcagtgata ctcgcctgag
     3361 aaccatgtac tttattgacg ctcacgccgg ctactttggg caggaaatca tacgccgagc
     3421 cgccttcgtt gaggagcagg ccgtttttga agggcggctg atcgaggccc agcaagccaa
     3481 tgccgaacgc atcggtgaag ctgccgtcgg actcctcggc gcccatatcg ccaaagactt
     3541 ccgagacaac ctgcaccaca tcgccgagcg ccaggctgcg gtcataagca ccgcaaatcc
     3601 ctgcattgat gatgagatcg tagcgctgtg cggccaaaat ttggcccatt gcgaaagcgg
     3661 tggcggtgag accaacgccg gtaacgagca gcgtgacatt gagccgtccg tactgaaact
     3721 gggactggcc atgcggcaca aaagcatttt tgaggtgctg ctccaatggc aatatttcga
     3781 aaggcgtggc agcaacgatg agcagcttca ttttttaatt ggtttggaat aggtgagacc
     3841 aaaggccggc gtgatgccga gataggaatg ccaggcagcg gcaaaatcga aagcgaggcc
     3901 gccgggcagg gtgtatccgg cgccgaggct ggccgatgca ggctgagtgg atgcgcccaa
     3961 acggaggaat acctgctcgt gcgggcgatc ctcgaagccg gttttggcgc gcagagggaa
     4021 gtcaatgtct ttttccaact caaagacaac gaggagttta tccgaacttt cgtaagccaa
     4081 gcccgtgcgc aggagggtgg gcaggcggtc gccgccgcga gtgacggttt gcaccggatt
     4141 ggccacatgc aggccgagga ggagcttggg cagcaggcgg gtttgtagcc cgatttcggc
     4201 gttgagggta agggtgttgc cgtagtcggg aatgcgggta gtgaacacgt tgatctgtgc
     4261 gccgatggaa gtgccgggga aaatttgccg cccgtaggcc aatccgaagc gctgctcatt
     4321 gtactctttc ataccgaaat actgcgccga cacggcaaaa gcaccgccgg aaacgggcat
     4381 ggcatacgcg gcgccgaggc tgcggaggtc ggccagcaga aagcgttgtt caccgaaaac
     4441 caatgccgcc gagcggtcga gcatgcccaa tccggcctga ttggacaggg cgctgtgtac
     4501 atcggtgaaa accacgccgg tgttccccat gcctatgccg cgggcgccgg cagcgggcgc
     4561 aatgccggtt tgggcttgaa gcgcagcaca gaacgccagt gcagtgatgc tgttgaaaag
     4621 aaaaagtaag tttgatttca tccggcattg gttttgccgg gctaaaatag gcagttcgct
     4681 gcaactggcg cgttttttct gcaccgattc ataagaactg gatgaggcgc atgtaaattg
     4741 caagccgcaa gcaccctttt cgaggcttgc ggcttgttca taaaatgata tttctcagcg
     4801 agttatccca gtgtaatggt gtatgtccgg ccgttgatgc tgagcgtagc cgtgccgtct
     4861 ccgttaaaaa cgatgctgcc ggtcgagttg atggtttggc catcggagcc ttgtcctgtg
     4921 atcaaaactt cggcgctgcc actctgtatt ttgcgagtgg ttttgctcac ggccacattg
     4981 gtcagggtga tggctaccgt gctggtagcg ccgttgccgt tgcgcagcag gcgagtctgg
     5041 ctgccgttgc gggtgcagga gccattgatg aggaagttgg ggccggtcac gaggttggaa
     5101 accgtgaggc tccccgtgcc attgtcattc gaacttactc tgggcgttgt gtaagtcccg
     5161 gaggattgga ggttcatgac gatgcgctgc ggaacgttga actgattgca ctggagcgtc
     5221 caattggctg atgctgtgta atctgcggtg atccgggtat tgctcaggct gcgactgaag
     5281 gtggtgtcgg cggtctgccc gcagctcagg gaaggcgcat cgagggtgaa tttttcggct
     5341 atgctcacca cttccgaaat accggcagca gcgccttccg aatccgtcat cagtgcgccc
     5401 tcaatgatta cggccacttc atcgggcgtg atgacagact gatctttttc acaggcggtg
     5461 agcgccaagg cgccgaagaa caaagcgaac gtcatcatga tggtgttttt tacaaacatg
     5521 tttttcattt tggtaaaaga ttttggtgat gaattaattg acggggcaaa gatgccgact
     5581 cgaaaccggg gactgcaaat cgtttcaacc aatggcgcca aatcttcaat gacctgtttg
     5641 aaagaggata tttggccccg ctcaggctgc ttttttaccg ttggtaggct gttccttacc
     5701 ggtcgttgaa aagcgtacgc gacttccatc gcgagatata tatttgtgtg gcaaacgaga
     5761 aaaaagatga aacatcacac ctattacacg gcagccgcca tctggatagt gatatggatc
     5821 atacacgcag gtgtgttcag cgtatttttt cctctgacaa tcagcattgt acgcagtgcc
     5881 gccaatgtag cgggcatgat gttgg
//
LOCUS       DAORLF010000304         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7361_length_5883_cov_1.997941, whole genome shotgun sequence.
ACCESSION   DAORLF010000304 DAORLF010000000
VERSION     DAORLF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7361_length_5883_cov_1.997941"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK198_19625"
     CDS             complement(<1..407)
                     /locus_tag="PK198_19625"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRF41015.1"
                     /translation="MSDWRQRTQLLIGETGIRQLEQAHVLVVGLGGVGSYAAEALVRA
                     GVGKLTIVDGDEVDATNKNRQLQALDSSIGKNKAALLKERFSDINPQAEIIALSTFLE
                     PDAFQALLQNDRFDYALDCIDSIQPKLNFIVLV"
     gene            complement(404..>819)
                     /locus_tag="PK198_19630"
     CDS             complement(404..>819)
                     /locus_tag="PK198_19630"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF41016.1"
                     /translation="AMNVQIAVLEQLLDIADRLERSVVIHCVRAYDPLLKIKKNFSRV
                     PHWAIHGYARHDVLAQSLVEQGFYLSVAPESTSHAGLLEAVRRIPADRLFLETDSAPA
                     QDIVSVYNFTAELCGIDLEYLKAIISENCKTFFQK"
     assembly_gap    820..931
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1096..>2547)
                     /locus_tag="PK198_19635"
     CDS             complement(1096..>2547)
                     /locus_tag="PK198_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HRF41017.1"
                     /translation="KAYFLADDPDGYTYDAVSMLMKHGVRVERATADFSVDAMNYRSG
                     KTERKTLPKGTFVIPAAQPRHLFLHSVMERNMAIEDSVMYDVSTWSVPLAFNLEAWAT
                     TGAWKANTEKLDKAPTLPSGLRTSDARYAYVIEWKQRHAPKALAALWEKGYRVRAAKE
                     PFSDGRTRYGEGTLIVLLGHNLEKADRMAQDMAEIAASCQVNIHGHHAGRMSDGFDLA
                     SSSNRPVARPKVALMVDPPFDMYSCGQIYFLFDQETRLPVERIRTSSLRQTALPKFGA
                     RYGYADLNNYQVLILPNGGMGLREVFQKEQLDQLRAWVEAGGVLIAQEGAVPFFTDNR
                     SKFTKVRLLETKPDTSTEQALFLPYAERSDYQGLKRVPGAALNATIDYTNPLAFGMGK
                     MLYSLKSGTEALKADADFQTVGRYATDPASLLASGYASQENLKHLAGNAFAGVLPMGR
                     GKVVFLLDNPQFRMYWRGPSRMMQNAVMLLPGF"
     assembly_gap    2548..2558
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2559..3747)
                     /locus_tag="PK198_19640"
     CDS             complement(<2559..3747)
                     /locus_tag="PK198_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF41018.1"
                     /translation="MRSFFLLPALCITILLRAQTPVLVDEQRFAFDPHISYDAAIPSP
                     QAFLGYPLGQEMTLHAHLVQYFEKLAASSPKISLNTYGKTYEGRPLINVVISSQANMN
                     RIEELRTQHLRLSDPDAISASEASQLIENQPVFLSMSYTIHGNEASSSEAVMQVAYRL
                     IAAQDEATRQLLDRSVIVLYICINPDGRDRFAYWYKSVQRSIPAHEPADLEHSEPWPM
                     GRTNHYWFDLNRDWFFMVNQESRAHSAEYQRWMPQVHADYHEQGYNSNYFTAPGTTPR
                     NLLMPKTYEALTDTFGRANIREFEKKQVSYFTREVFDFFYPGYGSSYPGVLGAVSMLT
                     EQGGIGGGRIVETEDGYMNTFRQRIWDHYATSMATLLKAGEMRKELLRYSFDAWNYRN
                     SASP"
     gene            complement(3996..4421)
                     /locus_tag="PK198_19645"
     CDS             complement(3996..4421)
                     /locus_tag="PK198_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41019.1"
                     /translation="MTFQLRSFRPVLFFAVIAMSVFALSFASQPAEDHAADSELTLLM
                     RQMYYDTASMKTAVKKRQKPVPAIQHKHLLTAVATEGKQTKSPAYQAMGQAYLKALEE
                     LLNASPKDAKDRYKKLIDSCMSCHTAFCPGPKMRIEKLY"
     gene            complement(4529..4669)
                     /locus_tag="PK198_19650"
     CDS             complement(4529..4669)
                     /locus_tag="PK198_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41020.1"
                     /translation="MKKAIALSKVVVFRPQKATLASRFAKHIEKENRVEKLPCEAVFI
                     VK"
     gene            4748..>5883
                     /locus_tag="PK198_19655"
     CDS             4748..>5883
                     /locus_tag="PK198_19655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41021.1"
                     /translation="MKSYLFISLFLVSFSPLWAQPPGGAAGTRPGAGPAIVGRISGIV
                     LDSINRQPVEFAAVALWDMKQDKQIDGIITDEKGHFRFPEVKAGKYKVQISFIGYQTK
                     ELSNLEITPQKPDVNVGQIMFSAEGLTLKEITVTGEAATIENKIDKMVYNAEKDVSTA
                     GGDAAEVLRRVPLLSVDLDGNVSMRGSENIMILINGRPSSMFAASVADALRTIPADQI
                     KSVEVITAPTARFDGEGSGGIINIITKRKSAQGVTGSASLAVGNRINRGNFNLNMAKG
                     RFGMNANISGWGSWKRPGFVDFYREDYLADNQVRTFTQNGSAVTQNYGPGGSVSAFYD
                     LNAYNTFSSNLSFRGHGDTRNEVVNSVFNDPLAGFNQTYTRTSNS"
BASE COUNT         1272 a         1463 c         1663 g         1362 t
ORIGIN      
        1 ctgaccaaaa cgatgaaatt caacttgggc tgtatgctgt ctatgcagtc caaagcgtag
       61 tcgaatcggt cgttttggag caatgcctga aaagcatccg gctccagaaa agtgctgagt
      121 gcaatgatct ccgcctgcgg gttgatgtct gaaaatcgtt ccttcaacag tgcggctttg
      181 tttttcccga tgctgctgtc caatgcctgc aactgcctgt ttttattggt agcgtccacc
      241 tcatcgccgt ccacaatggt cagcttgccc acgcctgcgc gcaccaaagc ctcggcggca
      301 tagctgccca cgccgcccaa gcctaccacc agcacatgcg cctgctccaa ttgccggatg
      361 cccgtttcgc cgatcagcag ttgtgttctt tgcctccagt cgctcacttt tggaaaaatg
      421 ttttgcagtt ttcagaaatg attgctttca gatactctaa gtcaatgccg cacagttcgg
      481 cggtaaagtt atacacgctg acgatatcct gagcgggcgc gctgtcggtt tccagaaaaa
      541 gccggtctgc cggtattcgg cggacggcct ccaacagccc tgcatgggaa gtgctttccg
      601 gcgctacgga caaataaaac ccttgttcta caagcgattg agccagcaca tcgtgccggg
      661 cgtagccgtg tatggcccag tgcgggacgc gactgaaatt ttttttgatc ttcagcaagg
      721 gatcataagc ccgcacgcaa tggatgacca cgcttcgttc gagccggtcg gcaatgtcca
      781 ataattgctc caaaacggcg atctgcacgt tcatggcggn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagccaggg atgactgccg agggtgtaca
      961 gttcgccggt ttttccatcg ccccaatgcg aagaaatgat ctccagcaca tcggcttgtt
     1021 gctcgcagcg cgcccggtgc gtgtggaaat caataaaaag atcgggcgag aaggagggcg
     1081 gcatccacgc attttttaaa agcccggcaa cagcatcacc gcgttttgca tcatgcggga
     1141 cgggccgcgc cagtacatgc ggaactgcgg attgtcgagc aaaaacacca ctttgccgcg
     1201 ccccatcggc agtacgcctg caaaggcatt gcccgccaga tgcttcaggt tttcctgaga
     1261 cgcatagccg gaagccagca atgatgcagg gtcggtggca taacggccaa cagtttggaa
     1321 atccgcatcc gctttcaggg cttccgtgcc ggatttgagc gagtacagca ttttgcccat
     1381 cccgaaagcc agcggattgg tgtagtcaat ggtggcattc agcgcggcgc cgggtacgcg
     1441 ttttaatccc tgataatcag atcgttcggc gtaaggcagg aaaagcgctt gctcggtaga
     1501 ggtatcgggt ttggtttcca gcaatctcac tttggtaaac ttgctgcgat tgtcggtaaa
     1561 gaaaggcaca gcaccctcct gggcaatgag aacgccgccg gcttctaccc aggcacggag
     1621 ttggtccaac tgttcttttt ggaatacttc gcgcagaccc atgccgccgt tggggagtat
     1681 gagcacttga taattgttga gatcggcata gccatagcgg gcgccgaact tgggcagggc
     1741 agtctgcctg agtgatgaag tgcggatgcg ctccaccggc aggcgcgttt cctgatcgaa
     1801 gaggaagtaa atctggccgc aggaatacat atcaaaaggc gggtccacca tcagcgccac
     1861 tttgggccgg gccacggggc ggttgctgct ggaggcgagg tcgaatccat cggacatgcg
     1921 gccggcgtga tgcccgtgaa tattgacctg gcaggaggcg gctatctcgg ccatgtcttg
     1981 cgccatgcgg tcggctttct ccaaattgtg gcccagcaaa acgatgagcg tgccttcgcc
     2041 gtagcgcgtg cgtccatcgc tgaagggttc tttggcggcg cgtacccggt agcctttttc
     2101 ccacagggct gccagcgctt tgggagcatg gcgttgcttc cactcgatga cgtaggcgta
     2161 gcgggcatcg gacgtgcgga ggccggaggg cagggtaggg gctttgtcca atttttcagt
     2221 attggccttc catgcgccgg tagtagccca ggcttcgagg ttgaacgcca gcggtaccga
     2281 ccaggtggat acatcgtaca tcacagagtc ttcaatggcc atattgcgct ccatgacgct
     2341 gtgcaggaac aggtggcgcg gctgtgcggc aggaatgacg aaagtgcctt tgggcagggt
     2401 tttgcgctct gttttgccgc tgcggtagtt catggcatcc acactgaaat cggcggtggc
     2461 gcgctctacc cgcacgccgt gtttcataag catggaaacg gcatcgtagg tatatccgtc
     2521 ggggtcgtcg gccagaaaat aggctttnnn nnnnnnnncc ggcgaggcgc tgtttcggta
     2581 gttccaggca tcaaaactgt accggagcag ttctttgcgc atttcgccgg ccttcagcaa
     2641 ggtggccatt gaggtggcgt agtgatccca gatgcgctgc cggaaagtat tcatatagcc
     2701 gtcttccgtt tccacaattc tgcctccgcc gatgccgccc tgctcggtga gcatactcac
     2761 ggcgcccaat acgccgggat agctggaacc gtagccggga tagaaaaagt cgaatacctc
     2821 gcgggtgaaa taactgactt gttttttttc aaactcccgg atattggctc tgccgaaggt
     2881 atcggtgagc gcttcgtaag tcttgggcat caggaggttg cgtggcgtgg tgcccggcgc
     2941 agtgaagtag ttgctgttgt agccctgctc gtggtagtcg gcatgcacct gcggcatcca
     3001 gcgctggtat tcggcagaat gagcgcggga ttcctgattg accatgaaga accagtcgcg
     3061 gttgaggtcg aaccaataat ggttggtgcg gcccatcggc caaggttcgc tgtgttcgag
     3121 gtcggcaggt tcgtgagcgg ggatgctgcg ttgcaccgat ttgtaccaat atgcgaagcg
     3181 gtcgcggcca tccgggttga tacagatgta cagcacgatg acggatcggt cgagcaactg
     3241 ccgggtagct tcgtcttgcg cggcgatgag ccggtaagct acctgcataa cggcctctga
     3301 actggaggct tcgttgccgt gaatggtata actcatggag agaaataccg gttggttttc
     3361 aatgagttga ctggcttctg aggcagaaat ggcgtcgggg tcgctgaggc gcagatgttg
     3421 cgtgcgcagt tcttcgatgc gattcatgtt ggcctgcgag ctgatgacca cattgatgag
     3481 cggccttcct tcgtaggttt ttccgtaggt gttcagactg attttgggag aactcgcggc
     3541 gagtttttca aaatattgca ctaaatgcgc gtgcagcgtc atttcctgtc cgagcggata
     3601 gccgaggaat gcctgcggag agggaatggc ggcgtcgtag ctgatgtggg gatcgaaagc
     3661 aaagcgctgt tcgtccacta aaacaggcgt ttgcgcccgc agtaaaatgg tgatgcaaag
     3721 ggccggaagg aggaaaaatg atctcatgga tgtgatgttt tttttaagtt gttttttaac
     3781 ccaaatcagt aggcggtggg cggtagccgc gccgtggcgc ggtaagcgtg atggaacgcg
     3841 gtgggcggtt aatcggtagg cggtaggcgg tgtgcagtac taagcgtgct ggaacgcggt
     3901 gggcggtttt ctgtgcgcgt cttgcagcca gcagcttgtg gccagtagcc agcagctaac
     3961 agccagcagc taacagccgt tcccacccgt tgaaatcaat acaacttctc aatcctcatt
     4021 ttcgggcctg ggcagaaagc cgtgtgacaa ctcatgcaac tgtctatcag cttcttgtag
     4081 cgatcttttg catctttggg cgaggcgttc agtagttctt caagcgcctt gaggtatgct
     4141 tgccccatag cctgataagc cggggatttg gtttgcttgc cttcagtggc aaccgccgtg
     4201 agcagatgtt tgtgttgtat ggccggtacc ggtttttgtc gctttttgac ggcggttttc
     4261 atggatgccg tgtcgtaata catttggcgc atcagcaggg tgagttcact gtcggcggca
     4321 tggtcttcag cgggttggga tgcaaatgaa agtgcaaaaa cagacatagc aatgactgca
     4381 aaaaaaagca cggggcggaa ggaacgaagt tgaaatgtca tgtaggtttg gatatgagtg
     4441 aataaaatgg tcgttttgaa cacgcaatat tactacaaat caaggcaggc cagagccacg
     4501 gagtgatgca gagagccgtc gcttaacatt atttaacaat aaacacggcc tcgcagggca
     4561 acttttcgac gcggttttcc ttctctatgt gctttgcaaa gcgggaagca agggttgctt
     4621 tttgcggcct gaaaacgacc actttactga gcgcgattgc ttttttcaaa aacaccaagt
     4681 agtggggcaa aaataaatga ccattttatt ttttatcgcc cccaatcaac aaaaaaatca
     4741 tccgaacatg aaatcttatc tgtttatctc tctttttttg gtttcgtttt ccccactgtg
     4801 ggcgcaaccg cccggcggcg cagcaggtac tcgccccggt gcaggcccgg ccattgtagg
     4861 ccgcatttca ggcatcgttt tggattcaat caatcgccag ccggtcgaat ttgctgctgt
     4921 ggcgctctgg gacatgaagc aggacaagca aattgacggt atcatcaccg atgaaaaagg
     4981 ccattttcgc tttcccgagg taaaagccgg caagtacaaa gtacagatca gtttcatcgg
     5041 ctaccaaacc aaagaactga gcaatcttga aatcactcct caaaagccgg atgtaaatgt
     5101 cgggcagatt atgttcagcg cagaaggtct gacgctcaag gaaataactg tgaccgggga
     5161 ggcagccacc attgaaaaca agatagacaa aatggtctat aacgccgaaa aagacgtgtc
     5221 cactgccggt ggcgacgcag cagaagtgct ccgccgggtg ccgcttttat cggttgattt
     5281 ggatgggaat gtatctatgc gcggctccga aaacatcatg attttgatca acggacggcc
     5341 ttcttccatg tttgccgcca gtgtagccga tgcgcttcgc accattcctg ccgaccagat
     5401 caaaagcgtg gaagtcatca cggcgcccac ggctcgtttc gacggggaag gctccggcgg
     5461 catcattaat atcatcacca agcggaagtc tgctcagggt gtaaccggat cggccagtct
     5521 ggcggtgggc aaccgaataa accgcggcaa tttcaatctc aatatggcca aaggccgctt
     5581 tggtatgaac gccaatattt ccggctgggg ctcgtggaag cggccgggct ttgtggattt
     5641 ttacagagag gactatcttg ccgacaatca agtgcgcact tttactcaaa atggctcggc
     5701 tgtgacccaa aactacggcc ccggcggctc ggtaagcgct ttttacgatc tcaatgctta
     5761 caacacgttt tccagcaacc tttccttcag agggcacggc gatacccgca acgaagtggt
     5821 gaactccgtg ttcaatgatc cgctcgccgg tttcaatcag acctacacgc gcaccagcaa
     5881 ttc
//
LOCUS       DAORLF010000305         5863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7412_length_5863_cov_1.636364, whole genome shotgun sequence.
ACCESSION   DAORLF010000305 DAORLF010000000
VERSION     DAORLF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7412_length_5863_cov_1.636364"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>377
                     /locus_tag="PK198_19660"
     CDS             <1..>377
                     /locus_tag="PK198_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41022.1"
                     /translation="GTITITSAGAGSSSADKQYMVQRELCGNGEIVVKIESISNGRAG
                     LEIRADNAPGAVKAGLKTPLSSTLTRYLRNAPGTAQSASSINAIGHRWLRLVRNGSNI
                     QLFWSPNGTNWTSAALYTNMALP"
     assembly_gap    378..390
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <391..969
                     /locus_tag="PK198_19665"
     CDS             <391..969
                     /locus_tag="PK198_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41023.1"
                     /translation="IGIYVESNNASVATGVFSNLSVSEVNPPATTFSFAAGSLSADAG
                     EEVQVCVNIANPCACAPASVQVALQGNAAPHLSGFSTQTLQFQSGDMQKCFSLSTAAA
                     PGDGSYTLILQNPTGGNDAAIGAQSALAVAVTAGNADGLPLGRAGQGDVPGGYHHHWL
                     HSARLIKRTLLLLVATGKHGAAILCPNQSLAR"
     gene            926..>1388
                     /locus_tag="PK198_19670"
     CDS             926..>1388
                     /locus_tag="PK198_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41024.1"
                     /translation="MEQPSSAQTRVWPDEDTQYVVYVTDDQGHLHGIDTVNITVLQPG
                     ALRISPNPSYLCNGAPDTLRTSGSFATYLWSSGQTSADIVVSTPGTYRVTATSAEGCE
                     VVDSVVVQAFDFQAQLTPQNPVFCQDSIKFTATSNAHTMGWYVEGELMTYSN"
     assembly_gap    1389..1419
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1420..>3513
                     /locus_tag="PK198_19675"
     CDS             <1420..>3513
                     /locus_tag="PK198_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41025.1"
                     /translation="VMHWIAPVEAVFYGPGGCREVRHIEFSRAIEDVVVFPAQPVICF
                     DDTLTLTAAPGFATYRWYDDEGGYLLSNTPSVQVTQPMIHYIEVTDANGCVFGKEVEI
                     NYAPAIESEIAVDFTSICFQEPGFVPPPGVSRPKSTQQQCAALAALDAGEGFVTYVWS
                     NGASGRNIQVDEPGEYAVTITDASGCTFSPRPVIVAPCTDPPFAFTPGTAPVISCTTP
                     AKLSPGAGFAHYSWSDGSTLDTLTTTLPGLYTVTVTDFNGCQAVQKYQLDIADANATV
                     DLALAGNTTTLVADEDAVGGMTFVNADNDDKDKIFDIDDKDVTNGDDDLMRLRLKLDP
                     AGMTTRTVTLRALSGGNTVKIWRSNTKKAGEYILGTPVELTQPDGSFFVLDLWVEGVK
                     AHTRQRETILVLECAGPQASLCLQSDSVSLTIVGLKELAWKGRNNGFDGGANQSNTLD
                     TADPNFPGNLKNTDNSPLRSYRVFPDMRIINGTAFPSILTSLRDSVELCLVLSVPPTE
                     PFHLYLKTFDVDDPSANNKPVDPNDKTKNPGIYDATANTGTYVYGPPLTYDKDNDNRG
                     IIFQAPAAPCPFGLLEGDTNFDGMVSLSFQPGDTLKTLWFKTSLHPGDNYRVAAVFDS
                     LMLPRLQNEDAKHGIHIHDKYAGRVVLDANMVSPVLTVWRLFNLEVASMANLTGNNNK
                     VKTFIYDFDGGNNLAS"
     assembly_gap    3514..3660
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3661..4679
                     /locus_tag="PK198_19680"
     CDS             <3661..4679
                     /locus_tag="PK198_19680"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41026.1"
                     /translation="LNSSPPANGRFENGFFTLGMNGTLEGKIRNNGDTFLNVNAPQSG
                     AASLPGLKMDLILIPVPGDTIKGLVQEIQKSANGTFTFTVVLLNRTLQGVTNSAVFIV
                     SDGLPMNIAAINTTNNTITTNNLSLTAMLRDDDRVASNQILPYNLAQDAAGFIVELDK
                     FKDAYLQTSVIQPSGTIKFFPTIYESNYQLKIYNEMPSRWVEIGMNNPGVPNAVGVWY
                     GSICFGWQSGKWNRDADPNSETNGSVNLGATFSSTNNNSMSAGGNVSVIPFETFYDLQ
                     QNLNSSSLKASASFIHEIGHQFGLAHIIEQRLMHEDLTFNNNSGFVPQHINIIRCRRN
                     SPGN"
     gene            4708..>5121
                     /locus_tag="PK198_19685"
     CDS             4708..>5121
                     /locus_tag="PK198_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41027.1"
                     /translation="MKNKFKLFVLMLCLSPIVSDCQDLPSIQFFPFKDTIFPLDAMFA
                     NLIWGNSLKKKMRINKPDWNSTVQVRRIAPDTTTWLNRDPLSEVSGFGISDEDGTIEI
                     DLGYETKRYFMIKPAFPTIMGMLPDEFLSINNRILL"
     assembly_gap    5122..5211
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5212..>5475
                     /locus_tag="PK198_19690"
     CDS             <5212..>5475
                     /locus_tag="PK198_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41028.1"
                     /translation="TEFTFTVLDSPPADEAALYDFLIKNDLANYIHHHRYAPENIPLF
                     EEALLLAPETSSLKPFIHQAVADLLTRQLYNAPKPYSDEELAVL"
     assembly_gap    5476..5485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5679..>5863
                     /locus_tag="PK198_19695"
     CDS             5679..>5863
                     /locus_tag="PK198_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41029.1"
                     /translation="MYTFNKTYSIRRLFMALVFCAAAVQAVAQIRIDSVQCACASGGT
                     GSITVIAEGSAGPFTFL"
BASE COUNT         1450 a         1419 c         1362 g         1341 t
ORIGIN      
        1 cgggaaccat caccattact tctgccggtg cgggcagcag cagcgccgac aagcaatata
       61 tggtgcagcg cgaactttgc ggcaatggag aaatcgtagt aaaaatagaa agcatcagca
      121 atgggcgcgc cggcttggaa atacgcgccg acaatgcacc gggggccgtc aaggccggcc
      181 tgaaaacgcc tttgagcagt acgcttacgc gctacctccg caatgctccc ggtacggcgc
      241 aatctgcaag ctccattaat gctatcggcc accgctggct gagattggtg cgcaacggga
      301 gcaacatcca gttgttttgg tctccgaatg ggaccaattg gacatcagcc gctttatata
      361 ccaatatggc cctgcccnnn nnnnnnnnnn atcggcatct atgtggagag caacaacgcc
      421 tccgtcgcca ccggggtgtt cagcaacctg agcgtcagcg aggtcaaccc cccagccacc
      481 accttctcct tcgccgccgg cagcctgagc gccgatgcgg gcgaagaagt gcaggtgtgt
      541 gtgaacatag ccaatccctg tgcctgtgcg ccggcctcgg tgcaggtggc cttgcagggc
      601 aacgccgctc cgcacctgtc gggtttcagt acgcagaccc tgcagtttca gtcgggcgac
      661 atgcaaaagt gcttttcgct ctctaccgcc gccgcgccgg gcgacggctc ttacaccctg
      721 atcctccaaa accccaccgg aggcaatgac gccgccatag gcgcgcagtc ggccctcgcg
      781 gttgccgtca cagccggcaa cgccgacgga ctgcccctgg gccgggccgg acagggagat
      841 gtgccggggg gataccatca ccattggttg cattccgccc gactcattaa acggactctg
      901 cttttactgg tggccacagg aaaacatgga gcagccatcc tctgcccaaa ccagagtctg
      961 gccagatgag gatacacaat atgtggtgta tgtaaccgac gatcaggggc atttgcatgg
     1021 cattgatact gtaaacatca ccgtgttgca accgggagcg cttcgcatca gtcccaatcc
     1081 ttcgtatctg tgtaacggcg cgccggatac gcttcggaca agcggaagct ttgcaaccta
     1141 tctttggtca agcggccaaa cgagcgccga catagttgtt tctacgccgg gtacttatcg
     1201 cgttacggct acctctgccg aaggttgtga agtagtggat tccgttgttg tgcaggcttt
     1261 tgattttcag gcacaactca cgcctcaaaa ccccgttttt tgccaggatt ccattaaatt
     1321 tactgcaact tccaacgctc acaccatggg ctggtatgtg gaaggcgagc ttatgacata
     1381 cagcaacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtaatgcact ggatagcgcc
     1441 ggtagaagca gtgttttatg gccccggtgg ttgtagggaa gtcaggcata ttgagttttc
     1501 gcgcgccata gaggatgttg tggtttttcc ggcacaacct gtcatttgtt ttgatgacac
     1561 gctgacctta acggccgcgc cgggatttgc tacatatcgc tggtatgatg atgagggcgg
     1621 atatttactt tccaatacgc cttccgtgca ggtcacccaa ccgatgatac actatattga
     1681 ggtgacggat gccaacggct gcgtttttgg aaaagaggtg gagatcaatt acgctccggc
     1741 catcgaatcg gaaattgctg tggattttac ttccatctgt tttcaggagc cgggtttcgt
     1801 acccccgcca ggtgtgagca ggcccaaaag cacacaacag caatgcgccg cactggcagc
     1861 tttggatgcc ggcgagggct ttgttactta tgtctggagc aacggcgctt ccgggcgcaa
     1921 tattcaggta gatgagcctg gcgaatacgc cgtgaccatt actgatgctt ccggttgcac
     1981 gttctcgccg cggcctgtaa tcgttgcacc ttgcacagac ccaccgtttg ctttcacacc
     2041 cggtacagcg ccggtcataa gttgtaccac ccctgcgaaa ctgtcgccgg gagcgggttt
     2101 tgcacattat tcgtggtctg atggaagcac gctggacaca ctgaccacca cattgccggg
     2161 cctttatacc gttacggtca cggatttcaa cggttgtcag gctgtgcaaa aataccaatt
     2221 ggacatagcg gacgccaatg cgacagtgga cttggcattg gcgggcaata ccaccaccct
     2281 cgtcgcggac gaagacgctg tgggcggcat gacctttgta aatgccgata acgatgacaa
     2341 agacaaaatt ttcgatatag acgacaaaga tgtgaccaac ggtgatgatg acctgatgcg
     2401 cctccggctc aaattggacc cggcaggcat gacgacgcgt accgttactt tacgcgcctt
     2461 atcgggaggc aatacagtga aaatatggcg ctcgaatacc aaaaaagcag gagagtacat
     2521 cttgggaacg cctgttgaac tgacccagcc ggatggcagc ttttttgttt tggacttatg
     2581 ggtagaaggc gtaaaagcgc atacccggca acgggaaacc atactggtac tggaatgtgc
     2641 cggtccgcag gcttccctgt gtttgcaaag cgactccgtt tccctcacca ttgtagggct
     2701 gaaagaactg gcatggaaag gacgcaacaa tggatttgac ggaggcgcca accaaagcaa
     2761 taccctcgat accgccgatc ccaattttcc cggcaacctc aaaaatacag acaattcgcc
     2821 gctacgaagc tatcgggttt ttccggatat gcgcatcatc aacggcactg catttccctc
     2881 gatcttaaca tctctgcgag actctgtcga gttgtgcctt gttttatcgg tgccgccgac
     2941 agagcctttc cacttatacc tgaagacctt tgacgtggat gaccccagcg cgaacaacaa
     3001 gcctgtggac cccaacgaca agacaaaaaa tccgggcata tacgatgcaa cggccaatac
     3061 cggaacctac gtttacggcc ctccactcac ttacgacaaa gacaacgaca ataggggaat
     3121 catctttcag gcgcctgcag caccttgtcc ttttgggtta ttggaaggtg ataccaactt
     3181 cgacggcatg gtgagcctga gttttcaacc cggtgatacg ctcaagacgc tgtggtttaa
     3241 aacctcgctg catccgggcg acaattaccg ggtagctgcc gttttcgact cgctcatgtt
     3301 gccgcgtctg caaaacgaag atgcaaaaca tggcatacat atccacgata aatacgcagg
     3361 cagggttgta ttggatgcca atatggtatc gcctgtgctg acggtatggc gcctgttcaa
     3421 tttggaagtg gcttccatgg ccaacctgac agggaacaac aacaaagtaa aaacattcat
     3481 ttacgacttc gacggaggca ataatctcgc ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 atttgaacag ttcgcctcct gccaatggcc gattcgagaa cggatttttc acacttggga
     3721 tgaacggtac gctggaaggc aagatcagaa acaatggaga cacttttttg aatgtcaatg
     3781 caccacaatc tggcgctgcc tcattgccgg gtttaaaaat ggacctgata ctcattccag
     3841 taccgggaga cactataaaa ggattggtgc aggaaataca aaagagtgca aatggaacat
     3901 tcacttttac ggttgtcctg ctcaacagaa ccttacaggg agttactaac tctgccgttt
     3961 tcatcgtatc tgacggactg ccgatgaata ttgctgccat caacacaaca aacaatacca
     4021 ttactaccaa taacctgagc ctgacagcca tgctcagaga cgacgaccga gttgcaagca
     4081 accaaatttt gccgtataat ctggcgcaag acgcggctgg tttcattgtt gagcttgata
     4141 agtttaaaga cgcatatctg caaacaagtg taattcagcc atctgggaca attaaattct
     4201 ttccaacaat ttatgaatca aattatcaac taaaaatata caacgaaatg ccttcaaggt
     4261 gggttgagat aggtatgaat aaccctgggg tacctaacgc ggttggtgtg tggtatggta
     4321 gcatttgttt tggctggcaa tccggtaaat ggaataggga tgcagatcct aattcagaaa
     4381 caaatggttc ggtaaatctg ggtgcaacct tttcaagtac taacaataac tctatgagtg
     4441 caggggggaa cgtttccgta ataccttttg agacatttta tgaccttcaa cagaacttaa
     4501 atagttcaag tttgaaagcc tctgcaagtt ttatccacga aattggacat cagtttgggc
     4561 tggcacatat tatcgaacaa cgactaatgc atgaagatct gacgttcaat aataatagtg
     4621 gttttgttcc gcaacacatc aatattatca ggtgcagaag aaactcgcca ggtaattaat
     4681 attatttaaa acataaattt gcaagtaatg aaaaacaagt tcaaattgtt cgtgctaatg
     4741 ctctgtttgt ctccgatagt gagtgattgc caagatttgc ccagtattca atttttccct
     4801 ttcaaagaca cgatatttcc attggacgca atgtttgcca atttaatctg gggtaattca
     4861 ctaaagaaaa aaatgagaat taataaacct gattggaata gcacagttca ggttaggaga
     4921 attgcgcctg atacaacaac ttggctgaat agagaccccc tctcagaagt gagcggtttt
     4981 ggcatatcag acgaagatgg taccatagag attgatttgg ggtatgagac aaaaagatac
     5041 tttatgataa agcccgcctt tcctacaata atgggaatgt taccagatga gtttttgagc
     5101 attaataata gaattttact annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naccgaattc
     5221 acctttaccg tattagattc ccctcctgcc gacgaagctg ccctttacga cttcctcatc
     5281 aaaaatgatt tggccaatta tatccatcat caccggtatg cgccggaaaa tattcctttg
     5341 ttcgaggagg cattactttt agcgccggag acttccagtc tgaaaccctt tattcatcag
     5401 gcagttgccg acctattgac gcgccaactt tacaatgcgc ccaagcctta cagcgacgaa
     5461 gaactggcag tcttgnnnnn nnnnncgtat atcacatgag aaaagtagta gaatcgaaac
     5521 atccttatct gctgcaaaat ctgtccactg atttatctaa ctaccaattt gaactacagg
     5581 ccgtgaaggc gcgattacgg cgcaaataac cacctcattt catcatccgt ccaagttcag
     5641 aaagaacaca agcggcattc acccacaact ccggcattat gtacactttc aacaagacat
     5701 actccatccg gcgcctgttc atggcactgg tattctgtgc agcggcagtt caggctgtgg
     5761 ctcaaatccg tatagattcg gtgcagtgcg cctgcgcctc cggcggcacg ggcagcatca
     5821 cggtcattgc cgagggcagt gccggccctt tcacgttttt gtg
//
LOCUS       DAORLF010000306         5859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7418_length_5859_cov_1.981220, whole genome shotgun sequence.
ACCESSION   DAORLF010000306 DAORLF010000000
VERSION     DAORLF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_5859_cov_1.981220"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK198_19700"
     CDS             complement(<1..778)
                     /locus_tag="PK198_19700"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41030.1"
                     /translation="MEISGQVMDSLSRQPLPLALVYVTSDTNRAYLAFAQTDEAGRFR
                     MTLPEGRSHLLLHARYLGYEEKALPAHPARDVAVYLSPAKNVLSEVVVRQTRPPIVQQ
                     SDTTTYDVKAFADSTEYSVEDVLKKLPGIQVHPDGQISVNGKNVEKVLIEGSDMFGRK
                     YTLGTRNIRAGYLDKVEIIDHYQENPVLKPVNTSEAVVLNLKLRADKKTILNGTFHAG
                     AGAGTDGDAKGKAHLNVFSISKRLKSILLSSSGNMANVTGW"
     gene            complement(1258..2043)
                     /locus_tag="PK198_19705"
     CDS             complement(1258..2043)
                     /locus_tag="PK198_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41031.1"
                     /translation="MKQIWDFIHGRDHLSGTLRYAELKPEALLHTMEKLGQRISERFP
                     QSGLSQVATELSEVAVPIVQLTERLRNPVWPIRVLVVAGVILLFSVAVGLLWLALRLS
                     PAVENGLSELLQGIESGINEMIFLALALYFLATLEGRYKQMLALRSLHRLRSIAHVID
                     MHQLTKDPAVVLADQPDTASSPQRTLTPFLLTRYLDYCSEMLSIVGKLAALHVQHFDH
                     PNVLDGVTNVESITGSLSAKIWQKIMILDLSLSQEDVKSESGE"
     gene            complement(2139..>2772)
                     /locus_tag="PK198_19710"
     CDS             complement(2139..>2772)
                     /locus_tag="PK198_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41032.1"
                     /translation="SAEITLRHSRFYNTSGNGIVAFHSRITAENCLVYNNGANSVQLI
                     LGGDYNFTYCTLANYGVNAGTLGMSNFYCYDDPLVCQVRRDYRLNAQFVNSVIYGSRR
                     DQIELADITGRQTPAMFNVSFKDCVVRVDELLTRQSGRYSDFLSTICTPCTNGMSNSK
                     VFKDPNKGDYYLDSLSIAIGKAKPLPAIAIDLENRPRDAANPDAGCFERE"
     assembly_gap    2773..2793
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2794..3628)
                     /locus_tag="PK198_19715"
     CDS             complement(<2794..3628)
                     /locus_tag="PK198_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41033.1"
                     /translation="MFRLLLGIAFISAVLMAACDREEDFITAPDAKLEFSLDTLRFDT
                     VFTQVGSATYSFKVYNRHDRPIRISNIMLDGSSGIRFRLNVDGVPGNAHQNVEIYGRD
                     SIYVFAEVTIDPNQPLSVSPFVIEDGITFETNGNIQRVHLEAWGQNANYFPSRYNKGV
                     QVRLTCNNGQVVWDDPKPYVVYGVLAIDECTLVIPAGARVYVHGGVAQNNVNGIYNDG
                     VIFTQANGRIRVQGTAEKPVIIQGDRLEEPFENSRGQWNGIIIGKGSQGNEINYASVL
                     NS"
     gene            complement(3664..>3856)
                     /locus_tag="PK198_19720"
     CDS             complement(3664..>3856)
                     /locus_tag="PK198_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRF41034.1"
                     /translation="RHLCMHRNAVLERSPALKELWGDDSIFNARSMDVYITKIRRHLQ
                     HDPSVQIINVRGIGYKLIC"
     assembly_gap    3857..3908
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3909..4358)
                     /locus_tag="PK198_19725"
     CDS             complement(<3909..4358)
                     /locus_tag="PK198_19725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF41035.1"
                     /translation="MAKVKILLAEDELLLARITKDSLESRGYDVVHAANGRQALELFA
                     TQVPDILVLDVMMPQMDGFSLAAEIRRHNPAVPIIFLTARSQVEDVVKGFELGGNDYL
                     RKPFSMEELIVRIKALLQRPSPTDMQPPAEQIYRIGEYTFDPIKQLLT"
     gene            complement(4358..4855)
                     /gene="rfaE2"
                     /locus_tag="PK198_19730"
     CDS             complement(4358..4855)
                     /gene="rfaE2"
                     /locus_tag="PK198_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRF41036.1"
                     /translation="MSAFDNIQRKIQTWAQARQTTSEWQANGEKIVFTNGCFDLLHYG
                     HIHYLADAKDLGSKLIVGINSSDSVSRLKGPTRPINDDLTRLHNMAALEMVDLVAVFE
                     EDTPLDLIRLLHPDVLVKGGDWPVESIAGAADVLSWGGTVRSLPFVTGYSTTSIESKI
                     KTSGN"
     gene            complement(4976..>5859)
                     /locus_tag="PK198_19735"
     CDS             complement(4976..>5859)
                     /locus_tag="PK198_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41037.1"
                     /translation="EKLLRLKSPDIDRYQVLQLYMVMGGVPFYLEAVRGDQSAAQNIE
                     RMCFASDGMLRNEFDNLYRALFKNSERHVAVVRAIATKSKGMTRSEILETAKLPDSGA
                     STRLLDELEKSGFIRKYSPFQRQKRESLYQLVDFYSMFYLKFIENTDPVDENTWINMI
                     DNPAQRAWSGYAFEQVGLYHIRQIKKALGISGILTQTSGWRSKQKADGAQIDLVIDRR
                     DQVINLCEMKFSLTPFTITKAYADRLMHKIGVFKEETQTNKALHLTMITTRGLARNNW
                     SDTLVRHDLKMDVLFEE"
BASE COUNT         1293 a         1451 c         1587 g         1455 t
ORIGIN      
        1 cccagcccgt cacattggcc atgttgccgc tgctgctgag cagtatggat ttgagacgtt
       61 tggagatgga aaatacattg aggtgcgctt tgccctttgc gtcgccgtcg gttccggcgc
      121 cggcgccggc gtggaaagtg ccgttcagga tggttttttt atcggctctc agcttcaggt
      181 tgaggacaac tgcctcggag gtattcaccg gcttcagcac cgggttttcc tgataatggt
      241 ctatgatttc cactttgtcc aagtatccgg cgcgtatgtt tcgggtgccc agggtgtatt
      301 ttctgccgaa catatcggag ccttcgatca gcactttttc cacgtttttt ccattcaccg
      361 atatttgccc gtcggggtgt acctgtatgc cgggcaattt tttcaacaca tcttccacgc
      421 tgtactccgt cgaatcggca aatgccttga cgtcataagt ggtcgtatct gattgttgta
      481 cgatgggcgg gcgggtttgc cttacgacca cctcgctgag cacgtttttg gccggactga
      541 ggtaaacggc tacatcgcgg gcagggtgcg ccggcagcgc tttttcttcg tagcccaaat
      601 accgggcgtg cagcagcaga tgggagcggc cttcgggcaa tgtcatcctg aaacgcccgg
      661 cctcgtctgt ttgggcgaaa gccagatagg cgcggttagt atcggaagtg acatacacca
      721 aagccaaagg caagggctgc cggctgaggc tgtccattac ctgcccgctg atttccatat
      781 tagtttgagc cggtagtaca gtcgcaaaca gacaccccgc agccaccaag ccgcgacggt
      841 tttcctttcg ccgcgcgact gtcgggcggg cacgcgacag ctaagcgcga agggcagacg
      901 cgaggagttg tgtacaaaca gtagcccccg aaatgcaggc agtgtggcaa ttcactggtt
      961 ttttcgattc gttttttttc ggtcatcagg tttcgattta tgagttaaac aattgaaaaa
     1021 caaagcactg tgcttgcaaa agatgcaagc atacttcccc ctttctttca tcattgttgt
     1081 tgttcaagtg tttgtctcag aatcgtcgct gaggtggctt ttagcctgcg gtttgcgatt
     1141 cggatgcaat gttagggaat aaactgcaaa agcggaaaaa atattatggg gttaacagcc
     1201 actgccttcg tacccctcaa tctggctcaa agtccatttt ttgagtactt ggcgcactta
     1261 ttcgcccgac tccgatttga catcctcctg cgagagcgac aaatccagaa tcatgatctt
     1321 ttgccagatt ttagccgaca ggctgccggt gatggactcc acgttcgtca cgccgtctaa
     1381 cacattggga tgatcgaaat gctgaacgtg cagggccgct aattttccaa cgatggagag
     1441 catctcggag caatagtcca aatagcgggt cagtaaaaac ggggtgagag tgcgttgtgg
     1501 agaggaagcc gtgtcgggct gatcggccag caccaccgca gggtctttgg tgagttggtg
     1561 catgtctatg acgtgggcaa tactgcgcag gcgatgcagc gaacgcaagg ccagcatttg
     1621 tttgtaacgc ccttccagcg tagccagaaa atagagcgcc agggccagaa agatcatttc
     1681 gttgatgccc gattcgatgc cctgcaacag ttccgacaag ccgttttcta ccgccggcga
     1741 caggcgaagc gccagccata gtaggccgac agctaccgaa aacaacagga tcacgccggc
     1801 caccaccagc acgcgaatgg gccaaacggg atttcggagg cgttcggtga gttgtacaat
     1861 gggaactgcc acctcgctca gttctgttgc tacctgactt aggcccgatt gcggaaagcg
     1921 ttcggaaatg cgttggccca gcttctccat ggtgtggagc aatgcctccg gcttgagttc
     1981 tgcatagcgc agggttcctg aaaggtggtc tctgccgtgt atgaaatccc aaatctgttt
     2041 catgcccttt tggattttag ccgtcgcctg aaaacaaatg aaacattttt gaacgctctc
     2101 atttggcaat cagcacatta tcacatcagc acatttgttt actcccgttc aaaacacccc
     2161 gcatcaggat tggcggcatc gcggggacgg ttttccaggt caatggcgat ggccggcagc
     2221 ggtttggcct tgccgatggc gatggaaagg ctgtccaaat agtagtcgcc cttgttgggg
     2281 tctttgaaca ctttggaatt ggacatgccg ttggtgcagg gcgtacaaat cgtgctgagg
     2341 aagtcggaat aacgaccact ctggcgcgtg aggagttcgt ccacccgcac tacgcaatct
     2401 ttgaacgaca cgttgaacat ggccggagtc tggcggcccg taatgtcggc cagttcgatc
     2461 tgatcgcggc gggaaccgta gatgacggag ttgacaaact gcgcattgag gcggtaatct
     2521 ctgcggacct ggcacaccaa aggatcgtcg tagcagtaga aattgctcat gcctaatgtg
     2581 cctgcgttga cgccgtaatt ggccagcgtg caataggtga agttgtaatc gccgccgagg
     2641 atgagttgta cagaattggc gccgttgtta taaaccaaac agttttcggc ggtgatgcgg
     2701 gaatgaaacg ccacgatgcc gttgccggaa gtgttgtaaa aacgggaatg gcgcagcgtg
     2761 atctcggcgg aannnnnnnn nnnnnnnnnn nnnggctgtt gaggacagag gcgtaattga
     2821 tctcattgcc ctggctgccc ttgccgatga tgatgccgtt ccattggccg cgcgagtttt
     2881 caaaaggctc ttcgaggcgg tcgccctgaa tgatgacggg cttttcggcg gtaccctgca
     2941 ctctgattct gccattggcc tgtgtaaaaa tcacaccgtc attgtaaatg ccgtttacat
     3001 tgttttgtgc cacgccgcca tgcacataca cccgcgcgcc ggcaggaatg acgagcgtac
     3061 attcgtcaat agcgagcact ccataaacga cataaggttt gggatcgtcc cacactacct
     3121 gaccattgtt gcatgtaagt cgcacctgca cgcccttgtt gtaccggctg gggaaataat
     3181 tggcattttg cccccaggct tcgaggtgta cgcgctggat attgccgttg gtttcaaacg
     3241 tgatgccgtc ttcgatgacg aaaggactga cagagagtgg ctggttgggg tctatggtca
     3301 cttctgcgaa gacataaatg ctgtctcgac cataaatttc aacgttttga tgcgcattgc
     3361 cgggcacgcc gtccacattg agccggaaac gaatgcccga actgccgtct aacatgatgt
     3421 tggaaatgcg gatggggcgg tcatgccggt tataaacttt gaaagaatag gtagccgatc
     3481 cgacttgagt aaaaaccgta tcgaagcgga gcgtatccaa cgaaaactcc agcttggcat
     3541 ccggcgcggt gatgaaatct tcttcccggt cgcaggcagc catcagaacg gccgatataa
     3601 aagcgatacc caagagcaaa cgaaacataa gcaaatagtg attttgggcg aaggtagcga
     3661 tattcagcaa atcagcttat acccgatgcc ccgcacattg ataatttgca cggatgggtc
     3721 gtgttgcaaa tggcggcgta tttttgtgat atatacatcc atgctgcggg cgttgaagat
     3781 gctgtcgtcg ccccagagtt ctttcagcgc gggcgagcgt tcgagtacgg cgttgcggtg
     3841 catacagagg tggcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnngg tgaggagttg tttgatgggg tcaaaggtgt attctccgat ccggtaaatc
     3961 tgttctgcgg gtggctgcat atcggtgggc gaggggcgtt gcaacagcgc tttgatgcgc
     4021 acaatcagtt cctccatgct gaaaggcttg cggaggtagt cgttgccgcc cagttcaaaa
     4081 cctttcacca cgtcttccac ctgggagcgg gccgtgagaa aaatgatggg aacggcggga
     4141 ttgtggcggc gaatttcagc ggccagcgaa aacccgtcca tctgcggcat catcacatcc
     4201 aaaacgagta tatcgggcac ttgggtggca aataactcca atgcctggcg accgttggct
     4261 gcatgtacaa cgtcgtagcc ccggctttcg aggctgtctt tggtgatgcg cgccagcagg
     4321 agttcgtctt cggccagcag gatttttact tttgccatca gttgcccgac gttttaattt
     4381 ttgattcaat agaggtggta gaataacccg tcacgaaagg caggctgcgc acggttccac
     4441 cccaggacag cacgtcggcg gccccggcaa tggactccac cggccaatcg ccgcctttga
     4501 ccaaaacatc ggggtggagc aaacggatta aatccaaagg agtatcctct tcaaaaaccg
     4561 ccaccaagtc caccatttcc aatgccgcca tgttgtgcag gcgcgtcagg tcgtcgttga
     4621 tggggcgcgt ggggcctttg aggcggctca ctgagtctga ggagttgatg ccgactatca
     4681 gcttgcttcc caggtcttta gcgtcggcca agtagtggat gtgcccgtag tgcagcagat
     4741 cgaagcagcc attggtgaac acaatttttt cgccattggc ctgccattcg gaggtggttt
     4801 gccgggcttg cgcccaggtt tgtattttgc gttggatgtt gtcgaaagcg gacatggttt
     4861 tttggataat tacgttattg cgaagatagt gaaggggtga ctcggccaag atcgcagttc
     4921 gatagccacc ccttcactac gcatagaaat ttgtaataat tttcccaaag gtaagctatt
     4981 cctcaaacaa cacatccatc ttcaaatcat gacgcaccaa ggtatccgac caattgttgc
     5041 gcgccaaacc acgcgtagtt atcatcgtca gatgcagggc cttattcgtt tgagtttctt
     5101 ctttgaatac gccgattttg tgcatcaggc ggtctgcata cgctttagtg atggtgaaag
     5161 gcgtcagaga aaatttcatt tcgcagagat taatcacctg atctctccgg tcaatgacca
     5221 gatcaatttg tgcgccgtct gctttttgtt tgcttcgcca gccggaggtc tgcgtcagaa
     5281 tgccggaaat gcccagcgcc tttttaatct gccggatatg atacaatccc acctgctcga
     5341 aggcgtatcc gctccatgct cgttgggcag gattgtctat catgtttatc caggtgtttt
     5401 catctacggg gtctgtgttt tcgatgaatt tcaggtagaa catggaataa aaatctacca
     5461 actgatacaa gctttcccgc ttttgccttt ggaaagggga gtattttctg ataaatccgc
     5521 ttttttccaa ttcatccagc aaacgcgtgc ttgctccgct gtcgggtagt ttggccgtct
     5581 ccagaatttc agatcgggtc attcccttcg atttcgtagc aatggcccga acgacggcaa
     5641 catggcgttc tgagttttta aacaaagccc ggtaaaggtt gtcgaactcg ttgcgcagca
     5701 tgccatccga tgcaaaacac attcgctcta tgttttgggc ggcgctttgg tcgcctctta
     5761 cggcctccag atagaaagga acacctccca tgaccatgta cagttgaagc acctgatacc
     5821 ggtctatgtc ggggcttttc aggcgcagca atttttccg
//
LOCUS       DAORLF010000307         5854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7428_length_5854_cov_2.200552, whole genome shotgun sequence.
ACCESSION   DAORLF010000307 DAORLF010000000
VERSION     DAORLF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7428_length_5854_cov_2.200552"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2650
                     /locus_tag="PK198_19740"
     CDS             <1..2650
                     /locus_tag="PK198_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRF41038.1"
                     /translation="NMVFTPEEMVQRKHHFAMVDEVDSVLIDDARTPLIISGPVAQGA
                     DDEEYIVLKPYVERLLEAQRRLATQYLAEARNLFAKGDTGVQEGEAGMALLRVHRALP
                     KYRPLIKFLSEDGVKVMLQKTENVYMQEQSKNMHLVDEPLLFTIDEKNRGVELTERGA
                     EFLAKGQEDTSFFVMPDVASRIIEIDADEDLDKAQKDEAKNKLAQDFTVKSKRLHAMY
                     QLLKAYTLFERDEEYVVIEGQVKIVDEQTGRMMEGRRYSDGLHQALEAKENVKVGEIT
                     QTYATVTLQNYFRMYHKLAGMTGTAETEAGELWDIYKLDVVVIPTNRDIIRDDKEDLV
                     FKTAREKFNAVIDEISLLSQAGRPVLVGTTSVDISEKLSRMLHMRNIEHNVLNAKQHQ
                     REAEIVTKAGRQDAQGHGNVTIATNMAGRGTDIKLAPETLFDVQVRSDRSAKGGYRYT
                     LTQRGSGNVVEMDLDHPLATAYQLTPETKVAGGLAIIGTERHESRRVDRQLRGRAGRQ
                     GDPGTSQFYVSLEDNLMRLFQSDRIAGLMDKMGHKEGDVIQHSMVTKSIERAQKKVEE
                     NNFGIRKRLLEYDDVMNIQREAIYKKRRNALYGERLAVDLSNMFSFNIENVVAQNKAA
                     GDYDGFRRDALNVLGIDPDIQAADFKEASASELTERLEQQFYEFYHRKGHQIGDFLMP
                     QIRDIYANQSHQYKGIQYMFSDGGVHPLLVRSEIEKAVKTNGKSIQSDIEQAVSLSII
                     DDKWKEHLRSMDDLKEAVQAASFEQKDPLVVYKMEAYNQFEQLITEVNQDVSAYLAKG
                     GLLLPENRPLAPARERKTDMSRMRAGRSGGGRTEAAEAARAAAEGVSQAPKQETVKRM
                     EKKVGRNDPCPCGSGKKYKHCHGKDA"
     gene            2795..>3255
                     /gene="deoC"
                     /locus_tag="PK198_19745"
     CDS             2795..>3255
                     /gene="deoC"
                     /locus_tag="PK198_19745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRF41039.1"
                     /translation="MNLAQQFDFTLLSPDCTPEQIRQLCDLAQKHRFASVCIPPYFVK
                     QAAEIFAEGPVKVCAAVGFPMGYSATPAKVEEIKKALDEGAEEIDAVLNICAVKAANW
                     NYVRNDLDSMTTAVHLKGKSISITIEAGLLTESELAKVCELALEIKPNFIK"
     assembly_gap    3256..3270
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3310..3444
                     /locus_tag="PK198_19750"
     CDS             3310..3444
                     /locus_tag="PK198_19750"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41040.1"
                     /translation="MQRLKELLGGKIRIKASGGIRTAEDAARLVEAGAARIGITEVLI
                     "
     gene            3535..4677
                     /locus_tag="PK198_19755"
     CDS             3535..4677
                     /locus_tag="PK198_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRF41041.1"
                     /translation="MRRIWPLVAFLTVAASCKTPAPPFTSAPVFEPGKQAKNIILLIG
                     DGMGLGQMSAAMYTNNNRLAMAEFPVVGFHKTYSADQLITDSAAGATAFACGSKTYNG
                     AIGVSNDTLPCFTIMEEAQQKGLATGLIATAAIVHATPAAFLAHVNNRISYEDIALDF
                     VKNPADLLIGGGSQYFRNRESDQRDLYSELRKKNYYVSDHSMEDLLRAPFRADQGFAY
                     FTAEGQPGTYSQNRTYLPFATRYGLKYLAQRSNKGFFVMIEGSQIDWKCHSNDGKEAV
                     LETLDFDSAVREALEFAKDRDDTLVIVTADHETGGMAIQPGSKMGRLKLEFTTNSHTA
                     AMVPVFAFGPSAELFYGIYENTGIYTRMRQALGFSSDAGTAGGNAH"
     gene            4691..>5854
                     /locus_tag="PK198_19760"
     CDS             4691..>5854
                     /locus_tag="PK198_19760"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRF41042.1"
                     /translation="MMKYFLIGSLLMAGLTACKTASKPARPMEQYDAPPLIEQSSTIH
                     VPLNINVAEMEQAINQRLQGVIYEDNDPDDGDNLAVKVEKKDRIGFGVSGKDLQYRVP
                     MTLWFKYKAGITYLTGTAEISMQFRTSFDIRPDWSVQTLTQLESYEWLRKPKLQVGAI
                     QIPIGFIGDVVMKNSQKTITTAIDRMAQEQFGLRGQVELAWRRMFEPVLISPEYNTWL
                     TIQPKSISMTPLGMSAEAITATIMVESQPRIKIGAAPASMPPTPLPPLRFAEATHRDF
                     IVYLQAEVPYEEAERLAREQLNGETFSSGGRSVTVENIELYGKGSTIIVNTELSGSFK
                     GNVYLEGRPYFNAKRNTIDVSDLNFTLETQNFLYKTAGWLLKSNLKRTIQEKMD"
BASE COUNT         1538 a         1542 c         1560 g         1199 t
ORIGIN      
        1 caatatggtg tttacacctg aagaaatggt gcagcgcaaa caccattttg ccatggtgga
       61 cgaggtggac tccgtactca ttgacgatgc gcgtacgccc ttgattattt ccggaccggt
      121 agcgcaaggg gctgatgatg aggaatatat cgtactgaaa ccctatgtgg aacgtctgct
      181 ggaagctcag cgccgtttgg ctacgcagta tttggcggaa gccagaaatt tgtttgccaa
      241 aggcgatacc ggcgtacagg aaggcgaggc cggcatggcc ttattgcgtg ttcaccgggc
      301 attgcccaag taccgtccgc tcatcaagtt tttgagtgaa gacggcgtca aagtgatgct
      361 ccagaagacc gaaaacgtct atatgcagga gcagagcaag aacatgcact tagtggacga
      421 acctctgttg tttaccatag acgagaaaaa tcgcggggta gaactcaccg agcgcggcgc
      481 cgaatttttg gccaaagggc aggaagatac ctcctttttt gtaatgcccg acgtggcttc
      541 ccgaatcata gaaatagatg ccgacgagga tttggacaag gcccagaagg acgaggccaa
      601 gaacaagctg gcacaggatt ttaccgtgaa gtcgaagcgc ctccacgcca tgtatcagtt
      661 gctcaaggct tatacccttt tcgagcgaga cgaagaatat gtggtcatcg aagggcaagt
      721 gaagattgtg gacgagcaaa caggccgtat gatggaaggt cgccgttatt ccgacggctt
      781 gcaccaggca ttggaagcca aagagaacgt gaaggtgggc gaaattaccc aaacgtatgc
      841 caccgttact ttgcagaatt acttccgcat gtatcacaag ctggcaggta tgaccggtac
      901 cgccgaaacc gaagccggcg aattgtggga catctacaaa ttggatgtgg tggtgattcc
      961 taccaacaga gacatcatcc gcgacgataa ggaagacttg gtattcaaaa ctgcgcgcga
     1021 gaaattcaac gccgttattg acgagatttc tctgctcagc caggcaggcc gccctgtgct
     1081 ggtgggtact acctccgtcg atatttctga aaaactgagc cggatgctcc acatgcgcaa
     1141 cattgagcac aacgtactca acgcaaagca gcaccagcgc gaagcagaaa tcgttactaa
     1201 agcaggtcgt caggatgcgc aggggcacgg caacgttacc atcgccacca acatggccgg
     1261 ccggggtacc gacatcaaac tggcgcccga aaccctgttc gacgttcagg ttcgcagcga
     1321 ccgctcggcc aaaggcggct accgctatac cctcacgcag cgcggttccg ggaatgtagt
     1381 ggaaatggac ctggatcacc cgctcgcgac ggcctaccag cttacacccg aaaccaaagt
     1441 agccggcggt ttggccatca tcggtaccga acggcacgaa tcccggcgcg tggacaggca
     1501 gttgcgcggt cgcgcaggcc gtcagggaga ccccggcacc tcgcagtttt atgtgtcgct
     1561 ggaagacaac ctcatgcgcc tgttccaatc cgaccgcatc gccggcctga tggacaaaat
     1621 gggccacaaa gaaggcgacg tcattcagca cagcatggtg acgaaatcca tcgaacgcgc
     1681 acaaaagaaa gtggaggaaa acaacttcgg cattcgcaag cgcctcctgg agtacgatga
     1741 cgtgatgaac attcagcgcg aggccatcta taaaaagcgc agaaacgctt tgtacggcga
     1801 gcgtttggcg gtggacttga gcaacatgtt cagcttcaac atcgaaaacg tagtggcgca
     1861 aaacaaagct gcgggcgatt acgacggctt ccgccgggat gcgctcaatg tattgggcat
     1921 tgaccccgac atacaagctg ccgattttaa agaagcttct gcctctgaac tgacggagcg
     1981 cttagagcag cagttttacg agttttatca cagaaaaggc catcagattg gagactttct
     2041 catgcctcaa atcagagaca tctacgccaa tcagagccat cagtataaag gcatccagta
     2101 catgttttcc gatggcggcg tgcatccgct tttggtgcgc agcgaaatag aaaaagccgt
     2161 aaaaaccaac ggaaaatcca tccagagcga tattgagcag gccgtttcct tatccattat
     2221 tgacgacaag tggaaagaac accttcgcag catggacgac ctcaaggagg cggtgcaggc
     2281 agcttctttt gagcagaagg accccttggt ggtgtataaa atggaggctt acaaccaatt
     2341 cgagcaattg attaccgagg ttaatcagga tgtttcggca tacctggcca aaggcggatt
     2401 attgctgcct gaaaaccgcc ccttagcccc ggcccgggaa cgcaaaaccg acatgagccg
     2461 tatgcgcgcc ggtcgtagtg gcggaggtcg cacagaggcg gcagaggcag cccgggcagc
     2521 agcggaaggc gtatcgcagg cgcccaaaca agaaaccgta aagcggatgg agaagaaagt
     2581 aggccgcaac gatccctgcc cctgcggtag cggaaagaag tacaaacact gccacggcaa
     2641 ggatgcgtaa tgtgtttgtt agattttttc taacaaaata cttttgggga gtggcggcgt
     2701 tcaggaaatt gagctaactt gcgcccgatt tctgacgcaa acgctttgag aaaccaaagt
     2761 cgcacgttta cttgcttaac acgagaatga tccgatgaac ctggcgcaac aatttgactt
     2821 caccctgctc agtcccgact gcaccccgga gcaaatccgg cagttgtgtg atctggctca
     2881 aaagcaccgc tttgcctccg tgtgcatacc cccgtatttt gtcaaacagg ctgccgagat
     2941 attcgcggaa ggtcccgtga aggtatgtgc agcggtgggg tttccaatgg gctactcagc
     3001 tacgcctgcc aaagtggaag agatcaaaaa ggcattggac gaaggcgcag aagaaattga
     3061 tgcagtgctc aatatttgtg ccgtcaaggc cgccaactgg aactatgtac gcaatgactt
     3121 agacagcatg acgacggcgg ttcacctcaa aggcaaaagc atcagcatca ccatcgaagc
     3181 cggtctgctc accgagtctg aattagctaa agtgtgtgag ctggcattag aaataaagcc
     3241 caattttatc aaaatnnnnn nnnnnnnnnn gctaccggct atcatggcga aggtgcgacg
     3301 gtacagtcgg tgcaacgctt gaaagaactg cttggcggca aaatccgcat caaagcatcc
     3361 ggcggcattc gcaccgcaga agatgccgcc cgattggtgg aagccggggc tgcccgcatt
     3421 ggaattacgg aggttctgat ttgatataat tcgtattgtt tttttagttt tgcagcatca
     3481 cccggcagga cttgggaatt tgggtgattc ccgaattggc aaaacaaaga aacaatgcgt
     3541 agaatatggc ctctggtagc ctttctgact gtggcggcaa gttgtaaaac gcccgcgccg
     3601 ccattcactt ccgcaccggt tttcgagccg ggcaaacaag ccaaaaacat cattttgctc
     3661 atcggtgacg gcatggggct ggggcagatg agcgccgcca tgtacaccaa caacaaccgc
     3721 ctcgccatgg ctgagtttcc ggtggtgggc tttcacaaaa cctattccgc agatcaactc
     3781 atcaccgact cggcagcagg cgctactgct tttgcctgtg gttcaaaaac ttacaacgga
     3841 gccatcggcg tctccaacga tactttaccc tgcttcacca tcatggagga ggcgcagcaa
     3901 aaaggcttgg ctaccggcct cattgccacg gcggccatcg tacacgctac gccggcagct
     3961 tttttggcgc atgtaaacaa tcgcatttct tatgaagaca ttgcgctgga ttttgtaaaa
     4021 aatccggccg acctgctcat tggcggaggc agccaatact tcaggaatcg ggaatccgat
     4081 caacgcgact tgtacagcga actgcgcaag aaaaattact acgtttccga tcacagcatg
     4141 gaagacctgt tgcgcgcgcc gtttcgggcc gatcagggct tcgcttattt tactgcggaa
     4201 ggccagccgg gcacctactc gcaaaacaga acctatctgc cttttgccac gcgttatggc
     4261 ctcaaatacc tcgcacagcg cagcaacaag ggcttttttg tgatgatcga aggctcgcag
     4321 atagactgga aatgccacag caacgatggc aaagaggccg tgctcgaaac gttggatttc
     4381 gactcggcgg tgcgggaggc gctggagttt gccaaagacc gcgatgatac cctcgtcatt
     4441 gttaccgccg atcacgaaac cggtggaatg gccatacagc ccggctcgaa aatggggcgt
     4501 ttgaagttgg aatttaccac caacagccac acagccgcca tggtgccggt ttttgctttc
     4561 gggccatcgg cagagttgtt ttacggaatt tacgaaaaca caggcatcta cacccgcatg
     4621 aggcaagcgc tgggcttttc atcggatgca ggcactgccg gcggaaatgc ccactaatat
     4681 cgttcacccg atgatgaaat attttctgat cggcagtttg ctgatggccg ggcttacggc
     4741 ctgcaaaact gcatccaaac cggctcgtcc tatggaacaa tacgacgctc cgccgctgat
     4801 tgaacaaagc tctaccattc atgtgccgct caacatcaac gtagcggaga tggaacaggc
     4861 catcaatcaa cggctccagg gagttattta tgaagataat gaccccgacg acggcgacaa
     4921 tctggccgtt aaagtagaga aaaaagaccg catcgggttc ggcgtttccg gtaaagacct
     4981 ccaataccgc gtacccatga ccctgtggtt caagtacaaa gccggcatta cctatctcac
     5041 cggcacggcg gaaatttcga tgcaattcag aacctctttc gacatccgac cggactggag
     5101 tgtgcaaacc ctcacgcagt tggaaagcta cgaatggctg cgcaaaccca aactccaggt
     5161 gggcgccatt cagattccca tcggattcat cggcgatgtg gtgatgaaaa acagccaaaa
     5221 aaccatcacc actgccatag accgtatggc acaggagcag tttgggctgc gcgggcaggt
     5281 agaactcgcc tggcgccgca tgttcgagcc ggttctcatc tcgccggaat acaacacttg
     5341 gttaactatt cagcccaaaa gcatctccat gacgccgctg ggtatgagcg ccgaggccat
     5401 caccgccacc atcatggtag aatcacagcc gagaatcaaa ataggcgccg ctccggcctc
     5461 tatgcctccc acgccgctgc cgccgctgcg ttttgcggaa gctacacacc gcgattttat
     5521 tgtgtatttg caagccgaag tgccctacga agaggccgag cgcttggccc gtgagcagct
     5581 caacggagaa acgttctcct ccggcgggcg ctctgtgacg gtagaaaaca tagagttgta
     5641 tggcaaaggc agcaccatca tcgtcaatac cgaactcagc ggcagtttca aaggcaatgt
     5701 atatttggaa gggcggcctt acttcaatgc caagcgcaat accatagacg tgagcgacct
     5761 caattttacg ctggaaaccc aaaattttct gtacaaaacg gcgggctggc tcctcaagag
     5821 caacctcaaa cgcaccatac aggaaaagat ggat
//
LOCUS       DAORLF010000308         5845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7452_length_5845_cov_2.175820, whole genome shotgun sequence.
ACCESSION   DAORLF010000308 DAORLF010000000
VERSION     DAORLF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7452_length_5845_cov_2.175820"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PK198_19765"
     CDS             complement(<1..1239)
                     /locus_tag="PK198_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRF41043.1"
                     /translation="MNNKALLLLAVFTALLSCPAFAQKVTDGRTAFEQMQFAVAAKLL
                     EKEYNKSKSRVEQGKLAFLLGESYRRINKSAQSIRWYQIAYNNQYGPDALRELAFALK
                     RAERYNDAAQAFKDLGIEIGSPYEYRREISACRTAEGWKKNSAAVAEYSVELMEFSGA
                     YSDFAPVPFRDGQVLFSSDRPGGSNDDTYAWTGNKFYDLYLADPKSGAVQLFDRAVNS
                     ENNEGTAAFNADFTEMIFSRCYGGKKEDAYCRLMVSDFRNGAWSNPRPLDFQIDKVNY
                     VHPSLSADGRFLYFASDHGDGWGGFDIYVSERKNGIWAEPSILSRSINTTGHEKFPFI
                     DNDTLYFASDYHPGMGGLDIFRSYKMANSAWSPPYNLLPPLNSGGDDFAFAIERKNPA
                     GSDVLETGYFSSARESGAGND"
     gene            1413..>1900
                     /locus_tag="PK198_19770"
     CDS             1413..>1900
                     /locus_tag="PK198_19770"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF41044.1"
                     /translation="MKTVLFAFVLLSAAVCDLSAQSPILLGQCTRDSLQQAPHREWFQ
                     KNYDEYAPDVSAIESLKNMIRKGFEVEIFFGTWCGDTRRELPRFIKILDLAGWDQSRV
                     RYIAVDSALPAYKQSPGGETLNKGIYRVPTFIIMKNGKEIGRIVEHPVKSLEADLLAL
                     TTG"
     assembly_gap    1901..1924
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1925..2292
                     /locus_tag="PK198_19775"
     CDS             <1925..2292
                     /locus_tag="PK198_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41045.1"
                     /translation="FAYSEIERLLTPGTLVHPNTSLRGIAKSLKPIVISWSELNACGY
                     VLQAQKKLPEALAVFRINHILFNENVDTHIALANSLYKNELYQEAVTTLQDAISLNKD
                     DGKLDKMLELYYAARKAQE"
     gene            2416..3519
                     /locus_tag="PK198_19780"
     CDS             2416..3519
                     /locus_tag="PK198_19780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF41046.1"
                     /translation="MLPVRKSSSALRCMHAKRPVSSLGTKKMASRAGLLRKAAIFKNN
                     LIALIGILLVWTCLGFFPNATAPEKAKAAPNVAQAVAENAARPVAEEASEQKTETTAT
                     RRQNHSFSTQRMSRIGGNSFYMGCAPNPKGCSNDERPMTRVTLSNYYIGRYEVTFSEF
                     DAYYRATGRRAFGDQGWGRGNHPVNCVSWLDAVKYCNWLSEQDGFTKVYDIQHHRAEV
                     IADWTADGYRLPTEAEWEYAAREGGRNVLFGNGSDWADPYEINFNPIVRNPNATFYRI
                     QTVPVGSLTSPNSLGLHDMSGNLYEWCWDWYGPYPGGKQNDYRGPRYGAFKVVRGGAF
                     VSEEAFIRTINRACVADSYQGGLVGFRLARTAK"
     gene            3645..3893
                     /locus_tag="PK198_19785"
     CDS             3645..3893
                     /locus_tag="PK198_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41047.1"
                     /translation="MHAARSESTPSSKLNLVQLHLLELFSKEMTEQELLDIKDLLTQY
                     YSRKVDEELNAIWENRNYSQASFTEATEELHLRAKNRP"
     assembly_gap    3904..4005
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4006..4315
                     /locus_tag="PK198_19790"
     CDS             <4006..4315
                     /locus_tag="PK198_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF41048.1"
                     /translation="ALTTEIIAEYEEVLNIFFESRTLGGNVCRVLLELPFTQRISIYY
                     NWHLISADPDDNKYVDCAVASNADYIVTNDSHFKILDKVDFPKVNCVSLADFMAILKP
                     "
     gene            complement(4354..>4888)
                     /locus_tag="PK198_19795"
     CDS             complement(4354..>4888)
                     /locus_tag="PK198_19795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF41049.1"
                     /translation="RDLNCDYYGASLHKWLSVPLGSGVLFVKKEKIPQIGPLFASAVT
                     DTSDIRRLNHTGTHPVATDLAVGNALDFYYALGPERKEARLRYLQQYWTDQVRNMPHI
                     VLNTPADPQRACGIANVGIRGMKPADMADTLLKKYRIYTVAIDGAGVQGCRISPNVYT
                     SLKELDVFVRALKEMGG"
     assembly_gap    4889..4933
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4941..5603)
                     /locus_tag="PK198_19800"
     CDS             complement(4941..5603)
                     /locus_tag="PK198_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF41050.1"
                     /translation="MDKRSFLKNTLLLGVGMPVFMNNLQGWAADIEHLSPEDAAKDEE
                     FWMRVRSGYRLKPDYINLENGYYCFLPQDILENYLHHIREVNYQGSHYMRTVQWDNKR
                     AMAAQLAEMLGCGADELIITRNTTESLDTIIGGFPWKPGDEALMAEQDYFSMLEMFKL
                     QSERHGVVNKRVSVPNHPRSDSEIVELYARAITPKTRLLMVCHIVNITGQVLRVDGGM
                     IV"
BASE COUNT         1318 a         1435 c         1552 g         1369 t
ORIGIN      
        1 gtcgttgcct gcgcccgatt cgcgggccga ggagaagtag ccggtttcga gcacatcgga
       61 gccggcggga ttttttcgct cgatggcaaa agcaaaatcg tcgcctccgg aattgagcgg
      121 cggcaggagg ttgtagggcg gcgaccaggc gctattggcc attttatacg acctgaaaat
      181 gtcgaggccg cccatgccgg ggtggtaatc ggaagcgaag tacagggtgt cgttgtcaat
      241 aaacggaaac ttctcgtgac cggtggtatt gatgctgcgg ctgaggatag aaggctcggc
      301 ccagatgccg tttttgcgtt ccgacacata gatatcgaag ccgccccagc cgtcgccatg
      361 atcggaggcg aagtacagga agcgcccgtc ggcagagagg ctgggatgca cataatttac
      421 tttatctatt tgaaaatcaa gcggtcgggg attgctccag gcgccatttc ggaaatcgct
      481 gaccatgagg cggcagtagg catcttcttt tttgccgccg tagcaacgcg aaaaaatcat
      541 ctccgtaaaa tcggcattga aggccgcagt gccctcattg ttttcggaat tgacggcccg
      601 gtcgaagagt tgcacggcgc ccgatttggg gtcggccaag tagaggtcgt aaaacttgtt
      661 gcccgtccag gcgtaggtat cgtcattgct gccgccgggc cggtcggaac tgaacagcac
      721 ttggccgtcg cggaaaggta cgggggcaaa atcggagtag gcgccgctga actccatcag
      781 ttccacgctg tattctgcga cggcggcgct gttttttttc caaccctcgg cagtgcggca
      841 ggccgatatt tcgcggcgat actcgtaggg gctgcctatt tctatgccga ggtctttgaa
      901 agcctgcgcg gcatcgttgt agcgttcggc gcgcttgagc gcaaaggcta attcgcgcag
      961 ggcgtcgggg ccgtattggt tgttgtaggc gatttggtac cagcggatgg actgcgcgct
     1021 tttgttgatg cggcggtaag attcgcccag caggaaggcc agcttgccct gctccacgcg
     1081 gcttttgctt ttgttgtact ctttttcgag caatttggcg gccacggcaa actgcatctg
     1141 ctcgaaagca gtgcgtccat cggtgacttt ttgcgcgaaa gcggggcagg agagcagtgc
     1201 cgtaaaaaca gcgaggagca gcaaggcttt gttattcata ggtctgtttg agttgtattg
     1261 gacttagaaa aacaagccct tgcggcggat tgttgccggg cggcggagcg gggttgcctt
     1321 taacaaacag ttcataacct ttttccaatt cattaacaag cccccggatg gctcgcgcct
     1381 acctttgcgg ctcaccttta aattcataca tcatgaaaac cgttctgttt gcctttgtcc
     1441 ttttgagcgc agccgtgtgt gacctgagcg cacagtcgcc gattttgctg ggccaatgta
     1501 cccgcgacag cttgcagcaa gcccctcacc gagagtggtt ccagaaaaac tacgatgaat
     1561 atgcgccgga tgtatcggcc atagaatcgc tgaaaaatat gatccggaaa ggcttcgagg
     1621 tagagatttt cttcggcacc tggtgtggcg atacgcgccg ggaactgcct cgcttcatca
     1681 aaatattgga tttggccggg tgggatcagt caagagtaag atacattgcc gttgacagtg
     1741 ctctgcctgc ttacaaacaa tcgcccggcg gcgaaaccct gaataaaggc atttaccggg
     1801 tgcctacctt tattattatg aaaaatggaa aagagattgg gcgcattgtg gagcacccgg
     1861 tgaaaagtct cgaagccgat ttgctggccc ttacaaccgg nnnnnnnnnn nnnnnnnnnn
     1921 nnnnacttcg cctactcaga gattgagcgc ctactgacac ccggcacgtt ggtacacccg
     1981 aatacctcgc tcagaggcat cgccaaatcg cttaaaccaa tcgtgatttc atggagcgaa
     2041 ctcaatgcct gcgggtatgt ccttcaggcg caaaaaaaac tgccagaagc gctggctgtt
     2101 ttccgaatca atcatatcct ttttaatgaa aatgtggaca cgcacatcgc attggccaat
     2161 agcttgtaca aaaacgaact gtaccaggag gccgtaacta ctcttcagga cgccatcagc
     2221 ctgaataaag acgacgggaa attggataaa atgctggaac tgtactacgc cgcccgtaaa
     2281 gcacaggagt gaaagtaaga gcgcgcctct gcgatgaatt gttatatgga taggagggca
     2341 ctcgttttta taaaaatgct atttttgcaa catccagttc ggttaattca tcattcatcc
     2401 actaaaacta ccgccatgtt gcctgttcga aagtcatcct ctgcactccg ttgtatgcac
     2461 gccaagcggc ctgtctcctc tttgggcact aaaaagatgg cctcccgcgc ggggctgttg
     2521 cgcaaagccg cgatcttcaa aaacaatttg atcgcgctga tcggcatcct tttggtttgg
     2581 acctgtctcg gctttttccc gaatgccact gcgcccgaaa aggccaaagc cgcaccgaat
     2641 gttgcacaag cagttgccga aaacgctgcc cggcccgtcg ccgaggaagc ctccgaacag
     2701 aaaaccgaaa caaccgccac ccgacggcag aatcacagct tctccacaca aagaatgtcc
     2761 cgcatcggcg gcaacagctt ttacatgggc tgcgcaccga acccgaaagg ctgctctaat
     2821 gacgagcgcc caatgacgcg tgtgacgctt tccaactatt atattggccg ttatgaagtg
     2881 acgttctctg agttcgatgc ttattacaga gctacgggcc ggcgcgcgtt tggagatcag
     2941 ggatggggcc gcggaaacca cccggttaat tgcgtcagtt ggttggatgc agtaaagtat
     3001 tgcaactggc tcagcgaaca agacggtttt accaaagtgt atgacattca gcatcatcgt
     3061 gcagaagtca tagccgattg gactgccgat gggtatcggc tgcccactga agcggaatgg
     3121 gaatatgccg cccgcgaagg tggacgaaac gttttgttcg gcaacggcag cgattgggcc
     3181 gacccctacg aaatcaattt caatcccatc gtacgaaatc ccaacgcgac tttttatcgc
     3241 atccaaaccg tgcctgtcgg ctctctgaca agccccaaca gcctcgggct acacgatatg
     3301 agcggcaatt tgtacgagtg gtgctgggat tggtacgggc cataccccgg cggaaaacaa
     3361 aacgactacc gaggcccccg atacggggcg ttcaaggtgg ttcggggcgg cgcttttgtc
     3421 agtgaagagg cattcattcg caccataaat cgcgcttgtg ttgcagacag ctaccaaggt
     3481 gggctggtcg gttttcggct ggctcgcacg gcgaaataag tgaagcgcct acacacggtt
     3541 cttcgatgca atacaatgga atgccaactt cttcgtatct tgctgaacgc aaatggggtg
     3601 caattcgtta ttattcatca atttcagtct caaaccggaa catcatgcac gcagcacgat
     3661 ctgaatcaac gccgtcttca aagttgaacc tcgttcaatt gcatcttctg gagctttttt
     3721 ccaaagagat gacagaacag gaattgctcg acatcaaaga cctcctcact caatattatt
     3781 cgcgcaaagt agatgaagaa ttgaatgcca tctgggaaaa tagaaattat tctcaagcgt
     3841 cgtttaccga agctacggaa gaactacatt tgagagcaaa aaacagaccc tgatgctcat
     3901 cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgc ttaccacaga
     4021 aataattgct gaatatgagg aggttctgaa tatatttttt gaaagtcgaa cattgggggg
     4081 caatgtttgc cgtgtgctgc tggaactacc gtttacgcag aggatcagca tttactacaa
     4141 ctggcatctt atttctgccg atccagacga caataagtat gtggattgtg ccgtcgcgtc
     4201 taatgccgac tacatcgtca ccaatgattc tcatttcaaa atcttggata aggttgattt
     4261 tccaaaggtc aattgtgttt ctttggctga ctttatggca atactgaaac cttagtactg
     4321 ggaaatctgt gccctccgaa ggagacaaac ctctcagccc cccatctcct tcagcgcccg
     4381 cacgaaaaca tccaactctt tcaggctggt ataaacattg ggactgatgc ggcagccctg
     4441 taccccggcc ccgtctatgg ctacggtata gatgcggtat tttttcagca gggtatcggc
     4501 catgtcggca ggtttcatgc cccggatgcc cacgttggca atgccgcagg cccgttgcgg
     4561 gtcggcgggc gtattgagta cgatgtgcgg catgttgcgc acttggtcgg tccagtattg
     4621 ttggaggtat cgcagacggg cttctttgcg ctccgggccg agggcgtagt aaaaatcgag
     4681 cgcattgccc accgccagat cagtggctac cgggtgcgtg cccgtgtgat tgaggcggcg
     4741 aatgtcggac gtatcggtaa cggcgcttgc gaaaagcggc ccgatttgcg gaattttttc
     4801 tttttttaca aataacaccc ctgaaccgag cggcacgctg agccatttgt gcaaactggc
     4861 gccgtagtaa tcgcagttca gatcgcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnntcagttc ctagacgatc atcccgccgt caacgcgcag cacctgcccg
     4981 gtgatgttga cgatgtggca caccatgagc aggcgggttt tgggagtgat ggcccgggcg
     5041 tagagttcta caatttccga gtcggagcgc gggtgattgg gcaccgatac gcgcttgttg
     5101 accacgccgt gccgttcaga ctgtagtttg aacatttcca gcatggagaa ataatcttgc
     5161 tcggccatca gagcctcgtc gccgggcttc catgggaaac cgccgatgat ggtatcgagc
     5221 gattcggtgg tgttgcgggt gatgatgagt tcatcggcgc cgcagcccaa catttcggcc
     5281 aattgggcgg ccatggcgcg tttgttgtcc cactgcacgg tgcgcatgta gtgcgagcct
     5341 tgataattga cctcgcggat gtggtggagg tagttttcca gaatgtcctg agggagaaag
     5401 cagtagtagc cgttttcgag gttgatgtag tcgggtttga ggcggtaacc gctgcgcacg
     5461 cgcatccaga attcttcatc tttggcggca tcctccggcg agaggtgttc gatgtcggcg
     5521 gcccagcctt gcaggttgtt cataaaaacc ggcattccca cgcccagcaa aagggtgttt
     5581 ttcaaaaaac tgcgcttgtc catgctgttc ttgttgtttc tttgatctga acttcgttgc
     5641 aggttccaaa agtaaacaat tgtggtacgg catcttcaac ccaagcgtta ccgtgggcat
     5701 ctctgtcttt taattctaca aaaattacag ttgaattgta tgcaacgccc agaaaggttc
     5761 aagatttttg ggtaaggacg ggtaattcca gcctcaagct tcaagctgct aacgctccgg
     5821 gttcaaggtt ccgggttccg cgttt
//
LOCUS       DAORLF010000309         5821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7494_length_5821_cov_1.949532, whole genome shotgun sequence.
ACCESSION   DAORLF010000309 DAORLF010000000
VERSION     DAORLF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_5821_cov_1.949532"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK198_19805"
     CDS             complement(<1..317)
                     /locus_tag="PK198_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF41051.1"
                     /translation="MSQVEFYTRFDQLTALLQAFAYNLTKNQEDAKDLYQETAFRALT
                     NRDKFHPGTNFKAWSFTIMKNIFINNYRKKVKANTIMDSTDNLYYLNSGSVSISNGAE
                     ANIL"
     gene            complement(519..>700)
                     /locus_tag="PK198_19810"
     CDS             complement(519..>700)
                     /locus_tag="PK198_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41052.1"
                     /translation="YTSHRQVRSRFCHTWRCHGAGAEGYAQNFAAQGIYDSALVLCGA
                     GIAISMLNIVIFNNF"
     assembly_gap    701..710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(728..>1394)
                     /locus_tag="PK198_19815"
     CDS             complement(728..>1394)
                     /locus_tag="PK198_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRF41053.1"
                     /translation="KKRLKLETKEQELQQTAQGNRAVEHLIREIREEKNLEKAREMAQ
                     KLREERQKLAQQVTVLQEEVYQQPEIKKSPIRKGDFVRLRTGSAIGRVEQVSKQKAVV
                     LMGDMRLTIKLLDLQHSNEHLEINSRASVQTDTIDQSAGFQSKIDIRGMRMDEAMRVV
                     EAFVDQAIMIGANNLRILHGKGDGTLRKAVRMKLREYHFPMDIKHPESSEGGDGVTLV
                     TLA"
     assembly_gap    1395..1419
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1420..3127)
                     /locus_tag="PK198_19820"
     CDS             complement(<1420..3127)
                     /locus_tag="PK198_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRF41054.1"
                     /translation="MLLEPKDLFERLEFDKVLELLTQLCLGELGREVTAAIRPETDLA
                     AIEQKLEEVSEFRRTIDRSDPFPITAYFDIAPDLRMLDIEDYVLSEEAFRRVNIVLLF
                     TRDIFRFFNPERRLLYPALYEIVRPIPFDDGLIKAIEKVIDEDGNIRPDASPELMRIR
                     KNILSKQGELDRVFRRIIQEARNNGWLSDNVESVRNGRRVLSVPSEHKRKIRGIIHDE
                     SATGRTAFIEPEPIIEINNDIFDLESEERREIYRILKELSATLRPYSPQMRDYLHLLV
                     RFDVLQAKARLAVQMHGLKPRVADKAQLGFIKSYHPLLLVKNKQLNRKTVPFTLHLHH
                     ENRLLLLSGPNAGGKSITMKSVGLMQLMVQCGLLVPAATESEFGIFHKFFADIGDQQS
                     IEDDLSTYSSRLANMRVFLENADRRTLVLIDEFGSGTDPKIGGAIAEAILRELNHRKV
                     AGVITTHYSNLKIFAFKTKGLVNGSMTFDKDSLSPTYELKIGRPGSSYAFEIAQKSGL
                     DAKVLEYARRRVGEQEGAVDQLLIDLQREKQELEEQITQLALKEQKADKLIKSYEQMY
                     RDL"
     gene            complement(3318..4097)
                     /locus_tag="PK198_19825"
     CDS             complement(3318..4097)
                     /locus_tag="PK198_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41055.1"
                     /translation="MKIVHILVVLLLVVLGTACQTKKDRVEKFVAQFNAATTKYFPET
                     QLLRGVSAKLKSENEVEIFIDVIVDTGSVAATLMNNYLPTVMELIVTDISEARNFIKE
                     GVVMKGYVRNQKDKTILKEMTINQGNYQDFMAKNKLGGPGDKAMSSKEKDLQLMLTRI
                     NQQFPVVDKESDLVINNVTLENGNTLVYHCIVGELYIDVYSMPNAEQIFKESILETPM
                     IKDGFKLVEPSGITGITYLYADASGKELLKIHFTAKEIKEL"
     gene            complement(4211..>4883)
                     /locus_tag="PK198_19830"
     CDS             complement(4211..>4883)
                     /locus_tag="PK198_19830"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF41056.1"
                     /translation="VPTLRTFNELDTPQNQVLFVDFPIVQADVLLVSKGTPAFSRDEH
                     LYRDWYNEYFGYGLSSIVFQEIREAKALAYSTYAMYSSPARSNRAHYLRAYVGTQPDK
                     LSDALPALLSIIEDMPVAEAQMEHARHSILKRIASERTLPSMLYWTARAWQDIGISTD
                     FRREIYDHLQTCSIQDLQAFQQQYVKGRAFTFLVLGSRDRVDMDYLASFGPVKELGME
                     EIFGY"
     assembly_gap    4884..4930
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4931..>5821)
                     /locus_tag="PK198_19835"
     CDS             complement(<4931..>5821)
                     /locus_tag="PK198_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF41057.1"
                     /translation="ITRMTYAFINGLPWEQMTRRIEIMQRLTKEDIVAFANRHLRPDN
                     YVVVYKRQGEDKGVIKVEKPPITSVTLNREGVSDFAREFLETATEPIQPVFDDFEAGI
                     QSYPLSNGLPFVYNPTNPLFRLDYIFEMGKSSDPAFAVAMSYLPYLGTSRKTGTQIKQ
                     ELFRLGLSFAATCNDERAYITLEGLEESLEEGLQLLEELLAGVQNDDPALRNIISDTL
                     LKRDNAKKDRNVILRDAMSAYARYGAQSPFTYRLSERELRALQPEELTGQISALCGLD
                     HRIYYYGTKDHNEVARLLEKY"
BASE COUNT         1284 a         1419 c         1498 g         1538 t
ORIGIN      
        1 aggatgttgg cttccgcgcc attggaaata gacacgctgc cggaattgag gtagtacagg
       61 ttgtctgtgc tgtccatgat ggtattggcc ttcaccttct tgcgataatt gttgatgaag
      121 atattcttca tgatcgtgaa cgaccaggcc ttgaaattgg tgccgggatg gaacttatcc
      181 cggttggtca atgcacggaa agccgtttcc tgatacaaat ctttagcatc ttcctgattt
      241 ttggtcaggt tgtatgcaaa agcctgcaaa agtgctgtca attggtcaaa tcgggtataa
      301 aattcaactt gagacatggc tgtaagattt ttgttgaaga atgatagcgc tcaatcgcct
      361 tattgttgaa acaaagatac aacacacttc aacaaaatgc ctaatttttt ttctattttt
      421 tttcaaaaaa agatgtacat acatctattt aatttattaa aatattgaaa atcaatcact
      481 aatgttaatt gttaattttt taaaaattca tcaaactttc agaaattatt gaaaataacg
      541 atattcagca tagatatggc aatgcctgcc ccgcacagaa caagcgctga gtcgtatata
      601 ccttgcgccg cgaagttttg agcatagcct tcagcacccg cgccatggca gcgccaggta
      661 tgacaaaagc ggcttcgcac ctgacggtgc gaggtataaa nnnnnnnnnn tggcaaaaat
      721 tggaaactta cgccagcgta accaaggtaa ccccgtcgcc gccctccgat gattcggggt
      781 gcttgatgtc catcgggaaa tggtactcgc ggagcttcat acgcacggcc ttgcgcaggg
      841 tgccgtcgcc tttgccgtgc aggatgcgga ggttgttggc gccgatcata atggcctgat
      901 ctacaaatgc ttccactacg cgcatggcct cgtccatccg cataccgcga atgtctatct
      961 tggactggaa gcccgccgat tggtcaatgg tgtcggtctg tacgctggct ctggagttga
     1021 tctccaagtg ttcgttggag tgttgcaaat ctaagagctt gatcgtgagg cgcatatcgc
     1081 ccataagaac gacagccttt tgtttgctca cctgttctac cctgccgatg gcgctgcccg
     1141 tgcggaggcg cacaaaatcg cccttacgga tgggagactt tttaatttcg ggttgctgat
     1201 acacttcctc ttgcagcacc gttacctgtt gggccaattt ttgcctttct tcgcgcaatt
     1261 tctgcgccat ttcgcgggcc ttctctaagt ttttctcttc gcgtatttca cgaatcaggt
     1321 gctctacggc ccggttgccc tgcgccgttt gttgcagttc ctgttcctta gtttccaatt
     1381 tgaggcgctt tttgnnnnnn nnnnnnnnnn nnnnnnnnnc cagatcgcgg tacatttgct
     1441 catacgactt gatgagcttg tctgccttct gttctttgag cgccaattgg gtaatttgct
     1501 cctccaattc ctgcttttcg cgctgcaggt ctatcagcaa ctgatccacc gcgccttcct
     1561 gctcccctac cctgcggcgc gcgtactcca gcactttggc atcgaggccg ctcttttggg
     1621 caatttcaaa agcataagaa ctacccggac gacctatttt cagctcgtaa gttggcgaca
     1681 ggctgtcttt gtcgaaggtc atggagccgt tgaccaagcc tttggtttta aaagcaaaaa
     1741 tcttgaggtt ggaatagtgc gtggtgatta cgccggccac tttccggtga ttcagttcgc
     1801 ggaggatggc ctcggctatg gcgccgccga ttttggggtc ggtaccggaa ccaaactcgt
     1861 caatgagtac aagggtacgc cggtctgcgt tttccaaaaa cacccgcata ttggccaacc
     1921 gggaactgta ggtactcagg tcgtcctcaa tggactgctg gtcgccgatg tcggcaaaga
     1981 atttatgaaa aatgccgaat tccgactctg tagccgccgg caccaagagg ccgcactgca
     2041 ccatgagttg catcaatccc accgatttca tggtgataga tttcccgcca gcattggggc
     2101 cactgagcaa aagcaggcga ttttcgtggt gcaaatgcag ggtaaagggc actgtttttc
     2161 gattcaactg cttgtttttc accaacaaaa gcgggtgata gctcttaatg aaccccaact
     2221 gtgctttgtc tgctaccctg ggcttgagac cgtgcatctg caccgccaac cgggctttgg
     2281 cctgcaacac atcgaagcgc acgagcaggt ggaggtagtc tctcatttgg ggcgaataag
     2341 gccgcagcgt ggcgctcaat tccttgagaa tgcggtatat ctcgcggcgt tcctccgatt
     2401 cgaggtcgaa gatatcgttg ttgatttcga tgatgggctc cggttcgatg aaggcggttc
     2461 ggccggtagc agattcgtcg tggatgatgc ccctgatttt gcgcttgtgt tcggaaggta
     2521 cgctgagtac tcggcgaccg ttgcgcacgc tttccacgtt gtcgctcagc cagccgttgt
     2581 tgcgggcttc ctgaatgatg cggcggaata ctcggtcgag ttcgccctgt tttgaaagga
     2641 tgtttttgcg aatgcgcatg agttcgggcg aggcatcggg gcggatattg ccgtcttcgt
     2701 caatcacttt ttcgatggct ttgatgaggc catcatcaaa agggatgggg cgtacaattt
     2761 cgtacagagc cgggtagagg aggcggcgtt cggggttgaa aaagcggaaa atatcgcggg
     2821 taaacagcag cacgatgttg acgcggcgga aggcttcctc gctgagcaca tagtcttcaa
     2881 tgtcgagcat gcgcaggtcg ggcgctatat caaagtaggc agtgatgggg aatgggtcgc
     2941 tgcggtctat ggtgcggcga aattcgctca cttcttccaa cttctgttct atggctgcca
     3001 ggtcggtttc gggccggatg gcggcggtca cttcacggcc caattcgccg aggcagagtt
     3061 gtgtgagcaa ctccaatact ttgtcaaatt ccagcctttc aaaaaggtct ttcggctcca
     3121 gtaacatgcg tttgttcgat ttcggcgcgc gccgcttcat tatataagga cattggaaca
     3181 ataggcaagg ccagagggtt ccggtggtga aaaacaaata ttcttcactc aaactctgct
     3241 gccaatctca ccaccaacca cttctcgcgc tgaaccccat atctttagat gctcgtgcag
     3301 agcagcaacc tctgacctca caactctttg atttcctttg cagtaaagtg tatttttaga
     3361 agttccttgc cactggcatc ggcataaagg taagttattc ctgtaatgcc cgaaggttcc
     3421 accagcttga aaccatcttt aatcattgga gtttccagaa tagactcttt gaatatctgt
     3481 tctgcgttgg gcatactgta aacatctatg tacagctcgc ccactataca gtgatacacc
     3541 aacgtattgc cattttccag tgtaacattg ttaataacca aatcactttc tttatccacg
     3601 accggaaact gctgattgat cctggttagc atcaactgta agtctttttc tttggaagac
     3661 attgccttgt caccgggtcc gccaagcttg tttttcgcca tgaagtcttg gtagttgccc
     3721 tgattgatgg tcatttcttt gagaatggtc ttgtcttttt ggtttctgac atagcctttc
     3781 atgacaacgc cctctttaat gaaattccgc gcctcgctaa tatcggtaac tatcaactcc
     3841 ataactgttg gaagataatt attcatcaaa gtagcggcta cggaccctgt gtccactata
     3901 acatcaataa atatttcaac ttcattttcg gacttcagct tagccgaaac acctctgagc
     3961 aactgcgttt cagggaaata cttagtagtg gctgcattga attgagcgac aaacttctct
     4021 accctatctt ttttcgtttg gcaggctgtt cccaacacga ccaaaagcaa tacgaccaaa
     4081 atgtgtacaa ttttcataga atactttttt gttttgtatc caaagttcaa tttcaaaatt
     4141 tgtcctcact tgctccggcg agtattctga aaatccggtc agcttttcaa accaactcat
     4201 taccaaagtt ttaataccca aatatctcct ccatgcccaa ctccttcacc gggccgaacg
     4261 aggctaaata atccatgtcc acgcggtcgc ggctgcccag aacgaggaag gtaaatgcgc
     4321 gacctttgac gtattgctgc tgaaatgcct ggaggtcctg gatgctgcag gtttggagat
     4381 gatcataaat ttcgcggcga aaatcggtgc tgatgccgat gtcttgccag gcgcgggccg
     4441 tccagtacaa catggaaggc agggtgcgct ccgaagcgat gcgtttgagg atggagtgcc
     4501 gtgcgtgttc catttgcgcc tcggctaccg gcatgtcttc tatgatgctg agcaaggccg
     4561 ggagcgcgtc ggagagcttg tcgggctgcg tgcccacata agcacggagg tagtgggcgc
     4621 ggttgcttcg ggccggcgag ctgtacatgg cgtaggtgga ataggccagg gctttggcct
     4681 cgcggatttc ctgaaaaacg atggaagaaa gcccgtagcc gaagtactcg ttgtaccagt
     4741 cgcggtagag gtgttcgtcg cgactgaacg cgggcgtacc tttcgacacc aacaacacgt
     4801 cggcctgcac gatgggaaag tccacaaaca agacctgatt ttgcggggta tctaattcgt
     4861 tgaaagtgcg caaggtaggt acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn gtacttctcc aacaggcgcg ccacctcgtt gtgatctttg gtgccatagt
     4981 agtaaatgcg gtgatcgagg ccgcacagcg cgctgatctg cccggtgagt tcctcgggtt
     5041 gcagggcgcg cagttcccgc tccgaaaggc gataagtaaa aggcgattga gcaccgtagc
     5101 gggcataggc gctcatggcg tcgcggagga tgacgttgcg gtcctttttg gcgttgtcgc
     5161 gtttgagcag ggtgtcggag atgatgttgc gcagggccgg atcgtcgttt tgcacgccgg
     5221 ccagcagttc ttccagcaat tgcagtcctt cctccaacga ttcctccaat ccttcgagcg
     5281 taatgtaggc gcgttcgtcg ttgcaggtgg cggcaaagga aagccccagc cggaacagtt
     5341 cctgcttgat ctgggtgccc gttttccggg aagtgccgag gtagggcaga taactcatgg
     5401 ccactgcaaa ggcggggtcg ctgcttttgc ccatttcaaa aatatagtcg aggcggaaca
     5461 gcgggttggt aggattgtaa acgaaaggca ggccgttgct cagcgggtag gattggatgc
     5521 cggcttcaaa atcgtcgaaa acgggctgaa tcggctctgt agccgtttcc aaaaactcgc
     5581 gggcaaaatc cgataccccc tcgcgattga gcgtcaccga ggtaatgggc ggtttctcca
     5641 ctttgatgac gcccttgtct tcgccctgcc gcttgtacac caccacatag ttgtcggggc
     5701 gcaggtgccg gttggcgaaa gccacaatgt cttctttggt caagcgctgc atgatctcta
     5761 tgcggcgggt catttgttcc cagggcaggc cgttgatgaa ggcatacgtc atgcgggtaa
     5821 t
//
LOCUS       DAORLF010000310         5771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7591_length_5771_cov_2.174423, whole genome shotgun sequence.
ACCESSION   DAORLF010000310 DAORLF010000000
VERSION     DAORLF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7591_length_5771_cov_2.174423"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK198_19840"
     CDS             complement(<1..685)
                     /locus_tag="PK198_19840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF41058.1"
                     /translation="MKSSHVMDRRLVVAGAILIQLALGAIYSWSVFTKILTDAEGIYR
                     FTATQTAWIFSAGLTTFAAVMVLAGKRLPSVGPRKLTVYGGLLLGLGYVLGGFFGSSF
                     WGQFLTIGVMAGAGIGMAYVVPIAVGVKWFPDKKGLITGLAVAGFGFGATIWVKLAGS
                     WFGGLLNYLQIGELPNVQSVFLLYGIIFAAFVLIGSLVMVNPPEGWLPAGWKPTAGPA
                     GSSGDEGFTP"
     gene            823..1413
                     /gene="ruvA"
                     /locus_tag="PK198_19845"
     CDS             823..1413
                     /gene="ruvA"
                     /locus_tag="PK198_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRF41059.1"
                     /translation="MISYIKGEITYKTPTYIIVETGGLGYHVLISLHTYAQIEKLERV
                     KIHTHLLIREDSHTLYGFAEESERSLFQHLISVSGVGPTTAQLLLSAMTPDDIRSAII
                     GENEQAFSKVKGIGPKTAKRIILDLKDKMVKSSGDAPLSLLPQDNTTREEALSALVAL
                     GFARVQVQKALNAVLKEKPSANNVEELIKLALRQLS"
     gene            1549..>3009
                     /gene="sprA"
                     /locus_tag="PK198_19850"
     CDS             1549..>3009
                     /gene="sprA"
                     /locus_tag="PK198_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF41060.1"
                     /translation="MKRLIPLSLVAVALLSAAFWPEDRSIRLDNPFRRALTAAPPAAD
                     TLPPLRPRFGDFLRDRNANPFDLKDPKEVKQDVEFDPITGQYILTERIGEEYYRPSTT
                     MSFEEYLDYRRKQDEKNYFDFLSGANTDGKINSLDPIAKIDVQNSLIDRLFGGTEVDI
                     KPQGNIDLTFGFDIQRLENPILLERQRTTGGFDFDMAIQMNVTGKIGSKLNLSTNYNT
                     NSTFNFDNQIKINYNTDALTDPFSEDAIIKKIEAGNVSLPLRGTLIQGAQSLFGLKTE
                     LQFGRLRLTSVISQQNSQKQNLKVEGGSQLVEFEVRADEYDENRHFFLSFFNQETFED
                     NLSNLPQVRSLFKLETMEVWVTNNRNEVDEVREIVAFADLGEPSTLVSPNKVNAPATP
                     VYRDITGTRGLPSNDANDLYARLNADRSMRQIDNVVAALQGPNFNLVQAQDFEKVFAR
                     KLKPSEYTVNNELGFISLGVNIQPDQVVAVTYSYSYN"
     assembly_gap    3010..3131
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3132..>3929
                     /gene="sprA"
                     /locus_tag="PK198_19855"
     CDS             <3132..>3929
                     /gene="sprA"
                     /locus_tag="PK198_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF41061.1"
                     /translation="LMMKNVYPIGAYQVDPKEFRLDVYYEDPGRGIKRFLPFSNLEGR
                     PLLRVFQLDKLNIQGDPQPDGIFDFVPGVTINTRNGRIMFPVLEPFGRSLAAQITEPA
                     LRDSLVYQALYDSTLFLAREFAEKNRFIIKGTYKSSVSSEISLGAFNIPPGSVSVTAG
                     GQLLIEGQDYEIDYNIGRVRILNDALLQSGVPVNVSYEDNTVFGLQSKTMMGIRADYE
                     ISKKLNIGATFMQLFERPFTPKVNIGDDPINNRIYGFDINYSTDAPFI"
     assembly_gap    3930..4118
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4119..>5771
                     /gene="sprA"
                     /locus_tag="PK198_19860"
     CDS             <4119..>5771
                     /gene="sprA"
                     /locus_tag="PK198_19860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF41062.1"
                     /translation="PINQWFLSSVPQGNNRWPEGNLINDLAYGANRGRMNWYRIDLSA
                     RNTGDTNNPYTSLVDQNEVFPNVQVTPDQLPNIQTFDLSFYPQERGPYNFDLPGGRMF
                     SRGVTFQGDSLILNDPRSRWGGVMRSLNTVDFQTANIEYLEFWMLSPFLDPTNPQNGI
                     LPQEVLDSLEGNLYFHFGNISEDILRDSRKFFENGLPSPFNPNRQVDTTAWGIVPVAQ
                     QITRFFDNDRQGGSESRKRQDVGLDGLDDDGEKAHFSEYIEAIRAVNPAVAAKIELDP
                     SNDNFRYFSDPSFGPNDGILRRYRDFNNPQGNSPENTGQLGLNSFASSTNIPDAEDIN
                     QDNTVNETEAYFQYRVPIKYDRGNPREIDTEATPFITDTRLAANGRIWYRFRVPLNSL
                     GQNEYTEAFGGIRDFRSIRFMRMMLKDFGRPVTLRFATIELVRNQWRRYQQDLSPSTN
                     CNNLTFNIDAVNIEENISRLPFNYVLPAGIVRERSLGVFNALQNEQSLVLQGQNLCDG
                     EEVAVFKTINLDMRMYKKLQMFVHAEDKQEPRVIQPGDLSIFI"
BASE COUNT         1376 a         1637 c         1319 g         1128 t
ORIGIN      
        1 cgggagtgaa gccttcatca ccgcttgagc cggccggtcc ggcagttggt ttccagccgg
       61 cgggcagcca gccttccggc ggattgacca tgaccaaact gccgatgagt acaaatgccg
      121 cgaaaataat gccgtacagc agaaaaacgc tttggacatt gggcaactct ccgatttgga
      181 ggtaattgag cagcccgccg aaccaggaac cggccagctt gacccagatc gtggcgccaa
      241 agccaaaacc agctactgcc aagcccgtga tgagaccttt tttgtcggga aaccacttca
      301 cccccaccgc aataggcacc acataagcca tgccgatgcc tgcgcccgcc atcacaccga
      361 tggtgaggaa ctgaccccaa aacgaagacc cgaaaaagcc gcccagcaca tagcctaagc
      421 ccagcaacaa gcccccgtaa accgtgagtt tacgcggccc cacgctcggc agccgctttc
      481 cggccagcac catcacagcc gcgaaagtgg tcaggccggc tgagaaaatc caggcggttt
      541 gggtagcggt gaagcgatag ataccttccg catcggtgag gattttggta aaaacagacc
      601 atgaatagat ggcgcccagg gcaagttgga tcaaaatggc gcctgccact acgagccggc
      661 gatccatgac gtgtgaggat ttcataaggt attgtcattg aatggactgc aatattggcg
      721 attcagagcc ttgtttacca atgataaagg tcattgggga atactgataa ttgtgtccca
      781 tgtaaaatcc ctatctttgc gccctcattt tcagcattgc acatgattag ttacatcaaa
      841 ggggaaatta cctataaaac gccgacatat atcattgtag aaaccggcgg tttgggctac
      901 cacgttctca tcagcctgca cacctacgcg caaatcgaaa agttggagcg cgtcaaaatt
      961 catacccatt tgctcatccg cgaagacagc cacacgctct atggctttgc cgaagagagc
     1021 gagcgcagcc tgtttcagca cctcatttcg gtgtccggcg tagggccgac cacggcgcaa
     1081 ctcttgctct ctgccatgac gcccgacgac atccgctcgg ccatcatcgg cgaaaacgag
     1141 caggcgttca gcaaggtaaa aggcataggc cccaaaaccg ccaaacgcat cattctcgac
     1201 ctcaaagaca aaatggtcaa aagctccggc gatgcgcccc tgtctttgct tccgcaagac
     1261 aataccacac gcgaggaagc gttatctgca ttggttgcgc tgggttttgc gcgtgtacag
     1321 gtgcaaaaag ccctcaacgc agtactcaaa gaaaagccct ccgccaacaa tgtagaggag
     1381 cttatcaaat tggctttgag acagttatct taggcgtatc aatgccgcat cccaaaaaga
     1441 aaacagctca gcaaaatgat tttgtgttaa agttccgtcg gcttgcgggc tggcggtgca
     1501 agcatcgttt ttagtacaca ttttgaaaaa aagcagacag acactctcat gaagcgtctt
     1561 atacctctca gccttgttgc cgttgccctg ctctccgccg ctttttggcc cgaagatcgc
     1621 agcatccggc ttgataatcc gttccgccgc gcattgaccg ccgcgccgcc cgccgccgat
     1681 accctgccgc ctctgaggcc gcgtttcggc gacttcctgc gagaccgcaa tgccaatcct
     1741 tttgatctca aagaccccaa ggaggtgaaa caggacgttg aattcgaccc gatcaccggc
     1801 cagtacatcc tcaccgagcg catcggcgaa gagtactacc gcccttccac taccatgtct
     1861 ttcgaggagt atctggacta ccgccgcaag caggacgaaa aaaattattt cgacttcctg
     1921 tccggcgcca acaccgacgg aaaaatcaac tccctcgacc ccatcgccaa aattgacgtg
     1981 caaaacagcc tgatagacag gcttttcggt ggcaccgagg tggacatcaa accgcagggc
     2041 aatattgacc ttactttcgg ttttgatatt cagcgcctcg aaaatcccat cctgctggaa
     2101 cgacagcgca ccacgggcgg ctttgatttt gacatggcca tccagatgaa cgtgaccggt
     2161 aaaatcggct ccaagctcaa cctctctacc aactacaaca ccaactccac gttcaatttc
     2221 gacaaccaga tcaagatcaa ctacaacaca gacgccctca ccgacccctt cagcgaagac
     2281 gctattatca aaaaaattga agccggcaac gtgagcctac ccctgcgcgg cacgctcatt
     2341 cagggcgcgc agagcttgtt tggtctcaaa accgaattgc aatttggccg cctgcgcctc
     2401 acctccgtca tttcgcagca aaactcacaa aagcaaaacc tcaaagtgga aggcggcagc
     2461 cagttggtgg aatttgaggt acgcgccgac gagtacgacg aaaaccgcca cttcttcctg
     2521 tcgttcttca atcaggaaac ctttgaagac aacctctcca acctcccgca ggtgcgctcg
     2581 ctgttcaagc tcgaaaccat ggaggtatgg gtgaccaaca accgcaacga agtggacgag
     2641 gtgcgcgaaa ttgtggcttt cgccgacttg ggcgagcctt ccaccctcgt cagtcccaac
     2701 aaggtaaacg cccctgccac acctgtctat cgcgacatca ccggcacgcg cgggctgccc
     2761 tccaacgacg ccaacgacct ctatgcgcgc ctcaatgcag accgaagcat gcgccagata
     2821 gacaacgtgg tggctgcgct gcaaggcccg aatttcaact tagtgcaggc gcaggatttt
     2881 gaaaaagtgt ttgcccgcaa gctcaagcct tcggaatata cagtaaataa tgagctgggc
     2941 ttcatctctt tgggggtcaa catccagccc gatcaggtgg tagccgtcac ctattcctat
     3001 tcctacaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nctgatgatg aaaaacgtct atcccatcgg cgcttatcag gtggacccca
     3181 aagagtttcg tctcgatgtg tattacgaag accccggcag aggcatcaag cgatttctgc
     3241 ctttttccaa cctcgaaggc cgcccgctgt tgcgcgtgtt ccaactcgat aaactcaaca
     3301 tacagggcga cccgcaaccc gacggcattt tcgactttgt gcccggcgtg accatcaaca
     3361 cccgcaatgg gcgcatcatg ttccctgtac tcgaaccttt cgggcgttca ttggcggcgc
     3421 aaataaccga gccggcgctg cgcgattcat tggtttatca ggcattgtat gacagtacgc
     3481 tgtttttggc cagggaattt gccgagaaga accggttcat catcaagggc acctataagt
     3541 cgagtgtgtc ttccgaaata tcgctcggcg cttttaatat tccgcccggt tcggtgagtg
     3601 tgacggccgg cgggcaattg ctcatcgaag gccaggatta cgaaattgac tacaatatag
     3661 gccgggtgcg cattctcaac gacgccctct tgcagtcggg cgtgccggtg aatgtgtcgt
     3721 atgaagataa cacggtattc gggttgcaat ccaaaaccat gatggggatt cgggccgatt
     3781 atgagatcag caagaaactc aatatcggcg ccacatttat gcagttgttt gaacggccgt
     3841 ttacacctaa agtgaacatc ggcgacgacc cgatcaacaa ccggatttac ggattcgaca
     3901 tcaattacag caccgatgcg ccgtttattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc catcaaccag tggttcctct
     4141 ccagtgtgcc gcagggcaat aaccgttggc cggaaggcaa cctcatcaat gacctggctt
     4201 atggcgccaa ccggggccgt atgaactggt accgcattga cctcagcgcc cgaaacacag
     4261 gagacaccaa caacccctac accagcttag tggaccaaaa tgaggtgttc cccaatgtgc
     4321 aggtcacgcc cgatcagttg cccaatattc aaaccttcga cctctcgttc tacccacagg
     4381 agcgcggccc gtacaacttc gacctgcccg gcggaagaat gttttccaga ggcgtcactt
     4441 ttcagggcga ctcgctgatc ctcaacgacc cgcgcagccg ttggggcggt gtgatgcgct
     4501 ccctgaacac ggtggatttc cagacggcga atattgagta cctcgaattc tggatgctca
     4561 gtcctttcct cgatccgacc aacccgcaga acggcatatt gccgcaagaa gtattggact
     4621 ctttggaggg caacctgtat ttccatttcg gcaatatttc ggaggatatt ctccgcgact
     4681 cccgcaagtt ttttgaaaac ggcctcccca gccccttcaa ccccaaccgc caggtggaca
     4741 ccaccgcatg gggcatagtg cctgtggccc agcagattac gcgctttttc gacaacgacc
     4801 ggcaaggagg ttctgaaagc cgcaaacgtc aggatgtagg tctggacggt ctggatgacg
     4861 atggtgaaaa ggcgcatttc agtgagtaca tcgaagccat ccgggcagtg aaccccgctg
     4921 tggccgccaa aatagaactc gacccatcca acgacaactt ccgctacttc agcgacccct
     4981 ccttcggccc caacgacggc atcctgcgcc gctaccgcga cttcaacaac ccgcagggca
     5041 actcgcccga aaatacggga caacttggcc tcaactcctt cgcatcttcc accaacatcc
     5101 ccgatgcaga agacatcaac caggataata ccgtcaacga aacggaggct tacttccagt
     5161 atcgcgtgcc catcaaatac gaccggggca atccccgcga aattgacacg gaggctactc
     5221 ctttcatcac cgatacccgt ctggcagcca atggccggat atggtaccga ttccgcgtac
     5281 cgctcaacag cctcggccaa aacgaataca ccgaagcatt cggcggcatc cgcgatttcc
     5341 gctctatccg cttcatgcgt atgatgctca aggacttcgg ccggcccgtg accctgcgtt
     5401 ttgccaccat cgaattggtg cgcaaccaat ggcgtcgcta ccagcaggac ctgtcgccga
     5461 gcaccaactg caacaacctg actttcaata tagacgccgt caacatcgaa gaaaacatca
     5521 gccgcctgcc cttcaactat gtgctgccgg caggcatcgt gcgcgagcgt tcattgggcg
     5581 tgttcaatgc tttgcaaaat gagcagtcat tggtattgca gggccaaaac ctctgcgacg
     5641 gcgaagaagt agcggtgttt aaaaccatca acctcgatat gcggatgtac aaaaaactgc
     5701 aaatgtttgt acacgccgaa gacaagcagg aaccccgcgt catacagccc ggcgaccttt
     5761 ccatcttcat c
//
LOCUS       DAORLF010000311         5764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7603_length_5764_cov_1.739709, whole genome shotgun sequence.
ACCESSION   DAORLF010000311 DAORLF010000000
VERSION     DAORLF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7603_length_5764_cov_1.739709"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>422)
                     /locus_tag="PK198_19865"
     CDS             complement(<1..>422)
                     /locus_tag="PK198_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41063.1"
                     /translation="APRWRPRPYWYDWGFYVRPGGGIVVFDFPSYYFVNWYYRRPIHV
                     YNYPYLAGLYVDYYYGYRRSSCGIVIGVDRWRRRNYAVVGDVWLRDRNGRVDRFREFG
                     RMEDERERFNRNNPGREIPQREYAVQNTTKYPRLTERLP"
     assembly_gap    423..474
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<475..>892)
                     /locus_tag="PK198_19870"
     CDS             complement(<475..>892)
                     /locus_tag="PK198_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41064.1"
                     /translation="IAMSDEQFGLLARVDNLQRESDAAFRNFLSNYPERTAAALRALV
                     NEPEAMEVMTENMRTTVQLGDLYNRDPYWMLQQADRISLDAARQRAKDLDAWKQSVEA
                     NPEARRELEQSAQTYAREYGYDDASYNYAPVERPPYV"
     assembly_gap    893..929
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<930..1281)
                     /locus_tag="PK198_19875"
     CDS             complement(<930..1281)
                     /locus_tag="PK198_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41065.1"
                     /translation="MKRMLIAIFALWAATQSMAQTREQLLEQLLEEDRANVETLMLYP
                     EETRQHILEASKHPEALIRMQTLQSRSQTAFRSLMETYPERVQQQVWDLTRYPGLIER
                     LVGEARGSRNAIIRL"
     assembly_gap    1546..1591
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1592..2072)
                     /locus_tag="PK198_19880"
     CDS             complement(<1592..2072)
                     /locus_tag="PK198_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41066.1"
                     /translation="MKYIFVLLLALSAVSLRAEFAVIAVTGSSLGNCTGSVTLRLNGD
                     AGPFGILIPETGFTLTDVEGDVVVNNLCTGNYTIMVSVSQFPGCSKSLSLFIPKLSDN
                     LSGITPTPRVVEDAEAKTVVGLTDVQKNIKVYPNPGRGMINLEFKNMHQYPIELLIFS
                     "
     gene            complement(2089..>4069)
                     /locus_tag="PK198_19885"
     CDS             complement(2089..>4069)
                     /locus_tag="PK198_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41067.1"
                     /translation="PNYESWRTGMQKGYRVYRKATDTLSGTVFQSFVSDTIYPVSESA
                     WQNPNNDDYIALARALCYDFSQSVYSDTLRAQDAYTAEEDRQNRFNFVLLVSDISSDA
                     ARQMGLSFVDTTINPGFIYEYKVLMVNSAHYSFYSAAINPADIPALPIPEQVEAEFAK
                     EHFFLSWSKIGIESFYTNYTIEYSEDMGATFQPMNEKPFVYLEADDLPESFQNKIIYK
                     DSISQIVPEYQFRVRGHTCFGETGPPSAIVSGRSVPDPLLVFPRITEVLEMADSSFQI
                     RWAINEAVADSITAIHIVRYEDVPGEAQRLTTTSLGLQERTYRDFQANNVNYYQVEIT
                     DIHGYIHPSPLGLGQRIDSIPPAIPLQLTGTIDSLTDSTMLVQLTWAANTESDLSGYY
                     VYRSNGRREEFAHLTTAGHLVETTFFDTIPAITLSDSIFYRLKAVDYRGNISPFSDIL
                     SLARPDFVPPAPPLLRYSPDADGGLSWVNSSSKDVVSYRIERRISGAENAWDVVESRA
                     KSRTSTDKIAAHVLEEEPGTVYAYRIIAIDDAGLETASNEVAVRESPLKKKFTSEFSF
                     DLNAQYSQGIVNLNWLNNYPFEIEKVIVYRSHNEGPYYSLMHVATLDLLPSSASRSGI
                     IDRHIEPGQKYFYKIQVKFKNGEYSNMSGVAIIRL"
     assembly_gap    4070..4079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4210..>5764)
                     /locus_tag="PK198_19890"
     CDS             complement(4210..>5764)
                     /locus_tag="PK198_19890"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF41068.1"
                     /translation="GFECEAEGGGSPSLEYEIISGVSPINMATGVAVYQQPVVDFNYP
                     VDNIVQLEEEGETRSYQVRIISFELRKNGDTLVSGQQVFSNGHYSMTFQPDEMFAPNT
                     QYVLSVTAGLFRSGVTAPIETEVKNDTFVTGEALRQIPLDNIALAWPANGQFNYYQGE
                     NVQNMIRLKTGQDYLFPQGVAAKVRLGGVQDETPAVYNSADNTVTYDLPTLPGGGAYE
                     AKLLVGDEEVLTWYFRVSKYGTFADKMNAVTMGSITPTNRYDDANINGLLEPFGEEET
                     NGISGLPDNLLSLRADLDNTLWMHQFSFMYQNTECNFSTANLDYDLWGIKRSAIEPFP
                     VNGVLLVQNSSNPFLVSEDNYGSLHENRDDNLLQIHQITPRIRYYIRSVVKNDFDKLS
                     DLAKNMGGILDLTNSFVREAFCGDSHPDLNTRITLSNQSLSTLYSSNSCYQQFYNFSP
                     LVSSAAPPDPHTDPCNATPFSFVSPFSPRAINTQPSYGMSQYPVVFKYQRPNSSPSSR
                     TFNLQYTQQ"
BASE COUNT         1441 a         1333 c         1405 g         1440 t
ORIGIN      
        1 ggcaggcgtt cggtgaggcg cgggtatttg gtcgtgtttt gaacggcgta ttccctttgc
       61 ggaatttccc ggcccggatt gtttctgtta aaacgctccc tttcgtcttc cattctgccg
      121 aattcgcgga agcggtccac cctgccgttt cggtccctga gccacacatc gcccacgact
      181 gcataattgc ggcggcgcca gcgatctaca ccgataacga tgccgcagga agagcgacga
      241 taaccatagt agtagtccac atacaggccg gcgaggtacg ggtagttata tacatgaatg
      301 ggacggcggt agtaccagtt gacaaagtag taagacggaa aatcgaacac gacaatgccg
      361 ccgccgggac gaacatagaa accccagtcg taccaatacg ggcgcggtct ccagcgggga
      421 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgtag
      481 ggcgggcgct ctacgggagc gtagttgtag gaggcgtcgt catagccata ttcgcgggcg
      541 taggtttggg cgctctgctc caattcgcga cgcgcttcag ggttggcctc tacgctttgt
      601 ttccaggcat cgaggtcttt ggcgcgctgg cgggcagcat cgaggctgat gcggtcggcc
      661 tgctgcaaca tccagtacgg atcgcgattg tagaggtcgc ccaattgcac ggtggtgcgc
      721 atgttctcgg tcatcacctc cattgcctcc ggttcgttca ccaaagcgcg caaagcagcg
      781 gcggtccgtt cggggtaatt actcaggaaa ttgcgaaaag cggcatccga ttctctttgg
      841 agattgtcaa cgcgggccaa caagccaaac tgctcatcgc tcatggcaat ggnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaggcgaatg atggcattgc gcgagccgcg
      961 ggcttctccc accaagcgct caatgaggcc cggatagcgg gtgaggtccc atacttgctg
     1021 ctgtacgcgt tcgggataag tttccatcaa agagcgaaac gccgtctgcg agcgggattg
     1081 cagggtttgc attctgatga gcgcttcggg gtgtttggag gcttccagaa tgtgctgtcg
     1141 tgtttcctcc gggtagagca tgagggtttc cacattggcg cggtcttcct ccagcaattg
     1201 ctccaacagt tgttcgcggg tttgtgccat gctttgggta gcggcccata aagcaaaaat
     1261 ggcgatgagc attcgtttca tagtttcttg ggttgatgag tgatgaaaag ccggggcatc
     1321 ttctcccccg acgatacgaa tatacggtaa tcggaggcga aaatgaaatg actttcgctg
     1381 aaatgccttg tttttaacag actttaaccg ggggtaacga agtgttaact atgattgcga
     1441 acggaatgcc ggctatgatt ttatcaccaa tgattgctgc acaaatgtac catcttcctg
     1501 acgggcaatg acgaagtaca agccggaagg aaggcgggtc aggttnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggaaaaga tcaacagttc tatcgggtat
     1621 tgatgcatgt ttttaaactc cagattaatc atccctctgc cgggattggg atatactttt
     1681 atattttttt gaacatcggt caaaccgact acggtcttgg cttcggcatc ttcaaccaca
     1741 cgaggcgttg gggtgattcc actcagatta tcagataatt taggaatgaa taaactcaag
     1801 gatttgctgc accccggaaa ctgagatacg cttaccatga tcgtgtaatt gcccgtgcac
     1861 aggttgttta caactacatc tccttccaca tcggtaagcg taaaccctgt ctcaggaatc
     1921 aaaatgccaa atggtcccgc atcgccgttc aatctcagag tgacactgcc tgtgcaattg
     1981 ccgagacttg aacctgtaac ggcaataacg gcaaattctg cgcgcagcga aacggcgctt
     2041 aaagccagca gcaaaacgaa aatgtatttc atggcaatca cttttgaatt ataacctgat
     2101 gatggcaaca cccgacatat tcgagtattc accgttctta aatttgacct gtattttata
     2161 aaaatatttt tggccgggtt caatatgcct gtcaataata cccgatctcg aagcactgct
     2221 gggcagcaaa tccaaagttg caacgtgcat caaagaataa taaggccctt cgttgtgaga
     2281 cctgtaaact atgacttttt caatttcgaa agggtaattg ttgagccagt tcaagttgac
     2341 gattccctgc gaatactgcg catttaaatc aaatgaaaat tcggaggtga atttcttttt
     2401 taacggcgac tctctcactg ctacctcatt ggaagctgtt tccaagcctg catcatctat
     2461 cgctattatt ctgtaagcat acaccgtgcc gggttcttct tccaaaacat gagcagcgat
     2521 tttatccgtt gaggtgcgcg atttcgctct ggattcgacg acatcccatg cattttccgc
     2581 ccctgatatc cttctttcaa tccggtaaga aacgacatct ttactcgaac tgttgaccca
     2641 actcagacct ccgtctgcat ccggcgaata gcgcagcaaa ggcggagccg gcggcacaaa
     2701 atcgggacgg gccaaagata aaatatcgga aaaaggagag atatttcctc tgtaatcaac
     2761 ggctttcagt cggtaaaaaa tcgaatccga taaagtaatg gccggtatcg tatcaaaaaa
     2821 tgttgtctca accaagtgcc cggcagtggt gaggtgcgca aactcctctc tgcggccatt
     2881 ggagcgataa acataatatc ctgataaatc gctttccgta ttggcagccc aggttaattg
     2941 taccagcatg gtagagtctg taagagaatc aatagtgcct gtcaattgca gcggaatggc
     3001 gggcggaatg ctgtctattc tttggcccag acccagcgga gaagggtgaa tatagccgtg
     3061 aatatcggtt atttctacct gataataatt gacgttattg gcttgaaaat ccctgtaagt
     3121 acgctcctgc aagcccaacg aagtcgtagt caaacgctgg gcctctccgg gcacatcttc
     3181 atagcgcact atatgaatcg ccgtgatact gtcggcgacg gcttcgttta tggcccaacg
     3241 aatttgaaaa ctgctgtcgg ccatttcgag tacctcggtg atgcgcggaa aaacgagcaa
     3301 gggatcgggt acgctcctgc cggaaacaat ggcggaaggc ggcccggttt ctccaaaaca
     3361 agtatgcccg cgcaccctga attgatactc cggcactatc tggctgatgc tgtctttgta
     3421 aatgattttg ttctgaaagg attcgggcag atcatccgct tccagataca caaagggctt
     3481 ttcattcata ggctggaagg tagcgcccat gtcttcagaa tactcgatgg tatagtttgt
     3541 ataaaatgac tcaatgccga ttttgctcca ggaaaggaaa aaatgctctt tggcaaattc
     3601 cgcctctacc tgctccggta tgggaagggc aggtatgtcg gccggattga tggcggcaga
     3661 gtaaaaagaa tagtgtgccg aattgaccat cagcacctta tattcataaa taaagccggg
     3721 atttatggtg gtgtcaacaa atgaaagccc catttgacga gcggcatcgg aagaaatatc
     3781 ggataccagc aacacaaaat tgaagcgatt ttgccggtct tcttcggcgg tgtatgcgtc
     3841 ctgtgctctg agtgtatcag aataaacgga ctgactgaaa tcataacaca aggcgcgcgc
     3901 cagagcgatg taatcgtcgt tgttcgggtt ttgccatgcc gactcggaaa caggatagat
     3961 tgtatctgat acaaacgact gaaaaaccgt tccgctcagc gtatctgttg cttttctgta
     4021 aacgcggtag cccttttgca tacctgttcg ccaggattcg tagttgggcn nnnnnnnnng
     4081 tccagcggac ggccgcaacc gaatcggaca atgcgcgggc tttgacgcct atgtaggccg
     4141 attgcgcacc cgcctgaacg ccgaacagca tcggcaaaat gcaaaaaacg atatatcgga
     4201 cgaatttttt cattgctggg tgtattgcag gttaaatgtt cggcttgatg gagaagaatt
     4261 ggggcgttgg tatttgaaca caaccgggta ttggctcatg ccatacgaag gttgtgtgtt
     4321 gatagctctt ggagagaagg gcgagacaaa agaaaatggt gtagcattgc aaggatcagt
     4381 gtgagggtca ggcggtgcag cactgcttac taatgggcta aaattataga attgctgata
     4441 acaagaatta ctgctataca aagtactaag cgattgattg gacaatgtaa ttcgggtatt
     4501 taaatcagga tgagaatcgc cacaaaaggc ttcacggacg aaggaattag ttaaatctaa
     4561 aatacctccc atgttttttg ccaaatcgga caatttatca aaatcatttt tgacgactga
     4621 gcgaatataa tacctgattc ggggggtaat ctggtgtatc tgcaacaaat tgtcatctct
     4681 attctcatgc agtgaaccat aattatcctc actaaccaaa aatggattgg acgagttttg
     4741 aaccagcaaa acgccgttta cgggaaatgg ctcaatagcg ctgcgcttaa tgccccataa
     4801 atcataatca agatttgcag tgctgaaatt gcattctgta ttttggtaca tgaatgaaaa
     4861 ttgatgcatc cagagggtat tgtctaaatc tgctctcagc gacaataaat tatccggcaa
     4921 gcccgaaata ccgttggttt cttcctctcc gaaaggctcc aacaagccgt tgatattcgc
     4981 gtcgtcatat ctattggtgg gagtaatact ccccatcgtc accgcattca tcttatccgc
     5041 aaaggtgccg tatttgctca ctctaaaata ccaggtaagc acttcctcat cgcccaccag
     5101 cagttttgct tcataagcgc cgccacccgg caatgtcggc aggtcgtacg ttacggtatt
     5161 gtcggcggag ttgtaaaccg ccggagtttc gtcctgtacg ccgccgagcc tgactttggc
     5221 ggctacgccc tgtgggaata aataatcctg tccggttttc agtcggatca tattctgcac
     5281 attttcgccc tggtaataat tgaattgccc attggccggc catgccaggg ctatgttgtc
     5341 gagaggaatt tgccggagcg cttcacccgt tacgaatgta tcgtttttca cttcggtttc
     5401 aataggtgca gttacccctg accgaaataa ccccgctgtg acggaaagaa catattgcgt
     5461 gttgggtgca aacatttcat ctggctggaa ggtcatggaa taatggccat tgctgaaaac
     5521 ctgttggccg ctgacgagcg tatctccatt tttacgcagc tcaaaagaaa taatccgcac
     5581 ctgatagctc cgtgtctcac cttcttcttc caattgcaca atattgtcaa ccgggtaatt
     5641 aaaatcaacc acgggctgct gatacaccgc tacgcctgtg gccatattga tgggcgaaac
     5701 accgctgatg atttcatatt ccagcgaagg gctgccgccg ccttcggctt cacattcaaa
     5761 gccg
//
LOCUS       DAORLF010000312         5719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7664_length_5719_cov_1.788489, whole genome shotgun sequence.
ACCESSION   DAORLF010000312 DAORLF010000000
VERSION     DAORLF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_5719_cov_1.788489"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>248
                     /locus_tag="PK198_19895"
     CDS             <1..>248
                     /locus_tag="PK198_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41069.1"
                     /translation="LIERCTFERDSCEDGGGGFQFTDLLHEGQVDVKSCRFISNYGNG
                     AGGLAFFSMPIYQKFTLRIENSEFVNNVNQLNGGGAIH"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            341..>638
                     /locus_tag="PK198_19900"
     CDS             341..>638
                     /locus_tag="PK198_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41070.1"
                     /translation="MFEMNRSNFAGGAFNIYTVKTSVSFINITNSQFLKNRSNTSWGG
                     ALYIWGQLDGAGAQSYTKIENCLFSGNRGAIGLTNGQSGIADMHIRNSTFYSNGT"
     assembly_gap    639..716
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <717..1077
                     /locus_tag="PK198_19905"
     CDS             <717..1077
                     /locus_tag="PK198_19905"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRF41071.1"
                     /translation="GLLFRRILSNNSPYYNLNMYRLDNCLVSAAECPDLPGSEQACGE
                     GMLYGLDPMFADSAGGDFRPLPCSPLLDAGNNLLLPTSDTDLAGLPRILGSTVDIGAY
                     EVPAFRIGASEILRSPA"
     assembly_gap    1089..1283
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1284..1771
                     /locus_tag="PK198_19910"
     CDS             <1284..1771
                     /locus_tag="PK198_19910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41072.1"
                     /translation="VVTPASNATATDGVALVQQITGGTVPYDIRWSNGASANGIGGLL
                     PGNYTVSITDANDCLFTQTVTVSFTNAVPETQVLPGVFRVAPNPSGSEGAQLWAEAAL
                     SGAVQVEILDALGRRAAAAIYLRGRNIDLPRGLSPGMYLIVLRDEQRRQAVLRWVVIK
                     R"
     gene            complement(1850..>2237)
                     /gene="bshC"
                     /locus_tag="PK198_19915"
     CDS             complement(1850..>2237)
                     /gene="bshC"
                     /locus_tag="PK198_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRF41073.1"
                     /translation="RYVAANTDQEISLAREKEQIQGAFAQILSKATALDPTLEKTVLG
                     EQAKQLNALDVLEAKLLRAEKQRQDTSVQQIRSLKDKYFPGNGLQERHDNFLPLYIRH
                     GQGFFELLRRELDPLKPGFVVVQEGA"
     assembly_gap    2238..2251
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2252..3398)
                     /gene="bshC"
                     /locus_tag="PK198_19920"
     CDS             complement(<2252..3398)
                     /gene="bshC"
                     /locus_tag="PK198_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRF41074.1"
                     /translation="MAYSGNDTALRPFFRYEVSLEAFAQVIEDKKKDPDHRAVLASAL
                     RTQYADLVQIPAVTAQLERLAQPHTFTVATAHQPCLFTGPLYYIYKAFSTIHLAGKLC
                     EAYPDYHFVPVFVSGAEDHDFDEINKARLFNKTIEWQNEEGGAVGGMSTATLSPALSE
                     LQAILGTSDAAQEAFALIEKAYTSHSGYGAATIHLLHALLGRYGLVVVDSHVAELKRL
                     FIPIMEKELLEQPSQALVQAAHDRLEAAGFSGQAFPREINLFYLRTGLRERIVLEDNL
                     YKVLNTSFVFTRDEILAELRAHPEHFSPNVVMRPLYQETVLPNLAYIGGGGEIAYWLE
                     RMDQFAHFGVNFPMLIRRNSLWWIDEAGAKRMQKLGLSPEDLFEDTES"
     gene            complement(3468..3713)
                     /locus_tag="PK198_19925"
     CDS             complement(3468..3713)
                     /locus_tag="PK198_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRF41075.1"
                     /translation="MDTILFFSLGGGEILLIFGAVLLLFGGKKIPELMRGIGKGIREF
                     NSARSTIESELKEGMREAERKELEEKSRKQEGPVERY"
     gene            complement(3803..>4585)
                     /locus_tag="PK198_19930"
     CDS             complement(3803..>4585)
                     /locus_tag="PK198_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41076.1"
                     /translation="PEGHEAYMEGFLKFPSVLALYDSCMLRYNKMDDIEKEFEQAFKY
                     FKHYFPQRPTPDVTTFISEYSIGTFIYGEASLAVGLDFFLGTDHPYAVYNPGNSNFSE
                     YLTRTFTREHLVSKTVQPLIEDMLGMPYGNRMLDMMIYNGKKLYLMDLLLPHTPDSIK
                     WEVTSKQAEWLNDNHLEMWAFFLSENLLYSTEWAKIRKYVEYSPNSPGMPEEAPGRTA
                     NWIGLQIVRAYMQQFPQTSPEQLLELRDSQDFLTKSRYKPRR"
     assembly_gap    4586..4847
                     /estimated_length=262
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4958..5689
                     /locus_tag="PK198_19935"
     CDS             4958..5689
                     /locus_tag="PK198_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF41077.1"
                     /translation="MKRKIAISAAFILTMIISGFAQNDVRFGFQVSPTVNWMSTNNNR
                     INSSGTNLGLKLGMTGDIYFRENYAVSTGLGFWFNAGGTLLHEYGGIYWDPALLPPGI
                     DTMPNMVKLKYGIQFVEIPIGLKMRTKEFGYNRYYIEPALTLGFKTQARGTATGNGLG
                     EEVEKINIRNEVNPLNLSWGIGGGIERSISENTSLILGAAFQIGFADLTRNRGQAFVP
                     NRTEPVREDSQGKVSGIILRAGIIF"
BASE COUNT         1171 a         1308 c         1412 g         1269 t
ORIGIN      
        1 ctgatagaga gatgcacttt tgaaagagat tcctgtgagg atggcggcgg cggatttcag
       61 tttaccgatt tgttgcacga agggcaggtt gatgtgaaat catgccgctt tatatcgaat
      121 tatggcaatg gcgcgggagg tctcgctttt tttagtatgc ctatttatca aaagtttact
      181 ttgaggattg aaaattcgga gtttgtaaat aatgtgaatc agctaaatgg tggtggagct
      241 attcattgnn nnnnnnnnca acgctaacca gtttgcagga ggtggagcct tatatatttt
      301 tgcaaacgga gatagtacat ttctccaatg tgataaatct atgtttgaaa tgaatcgttc
      361 gaactttgcc ggtggcgctt ttaatattta tactgtaaaa acaagcgttt ccttcattaa
      421 tataacaaat agccaatttt taaaaaacag atccaacacc tcttggggtg gcgctttgta
      481 catctgggga caattggatg gagcgggagc tcagagctat acgaaaatag aaaactgcct
      541 gttttccggc aatcggggcg ctataggtct gacaaacggg cagagcggca ttgccgacat
      601 gcacatccgc aacagcacct tttacagcaa cggcactgnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggc
      721 ctgctgtttc ggcgcatcct atccaacaac agtccgtact acaacctcaa tatgtaccgc
      781 ttggacaact gcctcgtcag cgctgcggaa tgtcccgacc ttccgggcag cgagcaggcc
      841 tgcggcgaag ggatgctcta cggcctcgat ccgatgtttg ccgacagcgc cggcggcgat
      901 tttcgcccgc tgccctgttc gccgctcctc gacgcaggca acaacctctt gctgccaacc
      961 tccgataccg atctcgctgg gctgcctcgc atcctcggca gcacagtcga tataggcgct
     1021 tatgaagtgc cggccttccg catcggcgca tcggaaatac tccgttcgcc cgcctgacac
     1081 gacagtgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnntcgtggt aacacccgcc tccaacgcaa cggcaacgga
     1321 cggcgtggcc ttggtacagc aaataacagg cggcacagtt ccttacgaca ttcgctggag
     1381 caacggcgct tctgccaacg gcatcggcgg cttgttgccg ggcaattaca cagtctccat
     1441 caccgatgcc aacgattgcc tgttcaccca aacggtgacg gtatcgttta ccaatgccgt
     1501 gccggagacg caggtattgc cgggcgtgtt tcgggtagcg cccaatccgt cgggcagcga
     1561 aggtgcgcaa ctttgggcgg aagccgcctt gtcgggcgct gtgcaggtag aaatattgga
     1621 tgctttgggg cggcgcgccg ctgcggctat atacctgcgc gggcgcaaca tagatttgcc
     1681 gaggggtttg tcgccgggca tgtacctgat cgtactccgc gatgagcaga ggcggcaggc
     1741 ggtgttgagg tgggtagtga taaaaaggtg agatggcggg ctgcgtgagg agctgaccgg
     1801 aagtcagctc ctcacttgca ggaggctgga tatcctgacg aggctccgct tatgcaccct
     1861 cctgcaccac cacaaaaccc ggcttcagcg ggtccaattc ccggcgcagc agttcaaaaa
     1921 agccctgccc atgccggatg taaagcggta agaaattgtc atgtcgttcc tggaggccat
     1981 tgccggggaa atacttgtct ttgagcgagc ggatttgttg cacggaagtg tcctggcgtt
     2041 gtttttcggc gcgcaagagt ttggcttcca gcacgtccaa tgcgttgagt tgtttggctt
     2101 gttcgcccag cacggttttt tctaatgtag ggtctaatgc ggtcgccttg ctcagtattt
     2161 gcgcgaaagc gccctgaatt tgttcttttt ccctggccag actgatttcc tgatcggtgt
     2221 tggcggccac atatcgcnnn nnnnnnnnnn ngcgattcgg tatcttcaaa gaggtcttcg
     2281 gggctgaggc cgagtttttg catgcgtttg gcgccggctt catctatcca ccacaaggag
     2341 ttgcggcgta tgagcatcgg gaaattgacc ccgaaatggg caaactgatc catgcgttcc
     2401 aaccagtagg ctatctcgcc gccgccgccg atgtaggcca aattgggcag caccgtttcc
     2461 tgataaagcg ggcgcatgac gacgttgggg ctgaagtgtt cggggtgagc gcgcagttcg
     2521 gccagtattt cgtcgcgggt gaatacaaag ctcgtattca gcactttgta cagattgtct
     2581 tccaacacga tgcgctcgcg gaggccggtc cggaggtaaa acaaattgat ctccctcgga
     2641 aatgcctgtc cgctgaaacc agctgcttcc aagcggtcgt gggcagcctg caccaatgcc
     2701 tgcgagggct gttcgagcag ttctttttcc ataatgggaa taaagagccg cttcaactcg
     2761 gcaacgtgac tgtcaaccac caccaagccg tagcgcccca acagcgcgtg tagcaggtgt
     2821 atggtagcag cgccgtagcc tgagtgcgag gtgtaagcct tttcgatgag tgcaaatgcc
     2881 tcctgcgctg catctgatgt gcccagaatg gcctgcaatt ccgacagcgc gggcgacagc
     2941 gtagccgttg acatgccgcc caccgcgccg ccttcctcgt tttgccactc gatggttttg
     3001 ttgaacagcc gcgccttgtt gatctcgtcg aaatcatggt cttcggcgcc gctcacaaat
     3061 acgggcacaa agtgatagtc gggataagcc tcgcagagct ttccggctaa gtgaatggta
     3121 gaaaaagcct tgtaaatata gtagagcggc ccggtgaaca ggcagggttg gtgtgccgtt
     3181 gcaacagtga aagtgtgagg ctgagccaat cgctccaact gcgcggtcac tgccggaatc
     3241 tgcaccaaat cagcgtattg cgttcggagg gccgatgcca gcacggcgcg gtgatcgggg
     3301 tcttttttct tgtcttcgat gacttgggca aaagcctcca gagagacttc gtaacgaaaa
     3361 aaaggcctca aagctgtatc gttcccggag taagccacat ctgttttaga gagttgtggt
     3421 actcgggaga aaggaataca cgatatctcc ataatgatat tccccgatca gtagcgctct
     3481 acggggcctt cctgctttct ggatttttct tccaattctt tgcgttctgc ttcgcgcatg
     3541 ccttccttga gttcggattc gatggtagaa cgggcagaat tgaactcgcg gatgcctttg
     3601 ccgatgccgc gcatcagttc gggaattttt ttaccgccga acagcagcag caccgcgccg
     3661 aaaatgagca aaatttcacc tccgccgagg ctgaaaaata agatggtatc catgattggc
     3721 atttttaaaa agcttattta tggtaaggta cgagcctcaa aacagactgc aatagtacaa
     3781 acttgttgtg aataaagttg acctacctcc gtggtttata tcggctcttg gtcagaaaat
     3841 cctgcgagtc acggagttcc agcagttgtt cgggcgaggt ttgagggaat tgctgcatgt
     3901 acgccctgac gatttgaagc ccgatccagt tggcggtgcg gccgggggct tcttcgggca
     3961 tgccgggcga attgggcgaa tattctacat actttcggat tttggcccat tcggtagagt
     4021 acagcaggtt ttccgaaagg aaaaaagccc acatttcgag gtgattgtcg ttgagccatt
     4081 ccgcttgttt ggacgtcact tcccacttga tgctgtcggg ggtgtgcggc agtagcaggt
     4141 ccatcaggta gagttttttg ccattgtaaa tcatcatgtc taacattcgg ttgccataag
     4201 gcatacccag catgtcctct atgagcggct gtacggtttt gctcaccaga tgctcgcggg
     4261 tgaaagtacg ggtgaggtat tccgaaaagt tggaattgcc cggattgtaa acggcgtagg
     4321 gatggtcggt gccgaggaaa aaatccaagc cgactgccag tgaggcttct ccatatataa
     4381 aggtgccgat ggagtactcg ctgatgaagg tagtgacgtc gggcgtcggt ctttgaggaa
     4441 aatagtgctt gaaatacttg aaagcctgct caaattcctt ttcaatgtcg tccattttgt
     4501 tgtagcgcaa catacaggaa tcgtagagcg cgagcacgga gggaaatttt aaaaaacctt
     4561 ccatatacgc ctcgtggcct tcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat ttctttcaat
     4861 cttgccttga gtatgtcgca ggtgattgta actttgcccg cgttttgggg cgcgaaatta
     4921 catccttgcg ccgtttctac acagtaaaaa acttaccatg aaaagaaaaa ttgccatttc
     4981 ggccgctttt atcctgacga tgattatcag cggctttgct caaaatgacg ttcgtttcgg
     5041 ttttcaggtc agcccgaccg tcaactggat gagtaccaat aacaaccgca tcaacagttc
     5101 cggcaccaac ctcggcctga aactcggcat gaccggcgat atttatttcc gcgaaaacta
     5161 tgctgtcagt accggcctcg gtttttggtt caacgccggc ggcaccctcc tgcacgagta
     5221 cggcggcatc tattgggacc ccgcgctgct gcctccgggc attgacacca tgcccaatat
     5281 ggtgaaactc aagtatggca ttcagtttgt ggaaattcct atcggactca aaatgcgtac
     5341 caaggagttt ggctacaatc gttattacat cgaacccgcg ctgacgctgg gcttcaaaac
     5401 gcaggcacgc ggcaccgcca ccggcaacgg actgggcgaa gaagtcgaaa aaatcaacat
     5461 ccgcaatgag gtcaacccgc tcaacctctc ctggggcatc ggcggcggca tagagcgcag
     5521 tatatctgaa aatacttcgt tgatcttggg cgccgctttc cagattggtt ttgccgatct
     5581 cacccgaaac agagggcagg catttgttcc caatcgcaca gagccggtac gggaggattc
     5641 gcagggcaaa gtcagcggta tcattcttcg cgcgggcatc attttttaaa actacacaac
     5701 atgatttcta tctccgatg
//
LOCUS       DAORLF010000313         5700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7699_length_5700_cov_1.878831, whole genome shotgun sequence.
ACCESSION   DAORLF010000313 DAORLF010000000
VERSION     DAORLF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_5700_cov_1.878831"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>985
                     /locus_tag="PK198_19940"
     CDS             196..>985
                     /locus_tag="PK198_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41078.1"
                     /translation="MKSWTLIFTMLFAVSFVSAQQQDTLWKKHFSAEELAHFDGQEDT
                     LALLAYVIVNDSLPEHRYSACHKLIPMLVSALKTPNSFRFPFERLKSVSIMYPPDSSF
                     RVFTWQLYVDKDEYRYYGAIQMNTKDLKLYPLKDRSFEIKGNLEQLELTPEQWYGVVY
                     YNIQQTDAKTGRYYLLFGFDGNEFFRKRKVLDVLTFKNGQPVFGAPVFRHENKKGEVS
                     FRKRRVIEYSAAASVRFNYDPALGIIIYDHLITMPGEDGEGPNNY"
     assembly_gap    986..1129
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1235..1894
                     /locus_tag="PK198_19945"
     CDS             1235..1894
                     /locus_tag="PK198_19945"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41079.1"
                     /translation="MHAHHFSVPRTAHFYSLGQPGPHSSECWVILHGYGQLAKYFLRR
                     FDAVADEHTCVAAPEALSRFYTDQEGIRVGASWMTKEQRLADIEDNMTYLDGLYDLLK
                     KDLPADCRFILLGFSQGCATLMRWVLRRQPPLAGLVLYAGSIPDDLPYATHHKYLNSF
                     PIHAVYGDADEYLNGERLAAWRGMLDGMHWQLRYHVFEGAHVVSRNVLAQLKQELNTP
                     I"
     gene            1977..2408
                     /locus_tag="PK198_19950"
     CDS             1977..2408
                     /locus_tag="PK198_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41080.1"
                     /translation="MKLRIHSFLGSWGLVSGIPAFILLLMAFVPYQRSAPSEREMQTA
                     FSLGCLERADIDFILTEPGVITAEAEWLPADSPAALLLYGPGQMNYYLRKEGKSGEKL
                     EFTVTDRHIARGTEWSVVVLNQQDTKISGLLKVRLPGEEPK"
     gene            complement(2486..3778)
                     /locus_tag="PK198_19955"
     CDS             complement(2486..3778)
                     /locus_tag="PK198_19955"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF41081.1"
                     /translation="MISKRILVVGLLMANCYLSTAQSDSSRQIKWLQPKPGAALKASA
                     MLQLCSIYTLGQEVYDATTLSYRPVDNRLNVYFRRARLLMRGEPYQGFKYYLAMHFDQ
                     AGHDLLSATQGPTNNAEPSVGIWDAFIQWQLPLGQDYLHLTAGWFRPQFQRESITAAW
                     SVSSFEKSMSQSYVRTHLVGRGSGRAAGINLGGMIKKEKLSLLYNAGIFNPLLTGLNG
                     NSVGIQYSPLLAGRLSLALGDPEMEQYGIAYGTNFFNERKGISIDLNIARQGKTDQFQ
                     NAVAWGPGLMLNYGRWNVDGEWMFLQRQGLRGEANIFTARSGTGHIRAGYNITVVKFM
                     LQPSAMLMRFEGAMDAAGQADARVLKLSSGREQTIDLGLNYYLRKRDMVIQLHYTLHS
                     GDAGTAGDGSTVNAYFAQNTVGAIRRGNWIGLGMNAIF"
     gene            complement(3932..4588)
                     /locus_tag="PK198_19960"
     CDS             complement(3932..4588)
                     /locus_tag="PK198_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41082.1"
                     /translation="MKKTISTLHYLVFTGVTALLLSGCVSMSTMQTGRTVGQGNSEVN
                     VGGSGVKYAFVLGIEELDTASVSAPMLEADFRYGVTDKLDIGGKVSLLGTSGLYGKYQ
                     FLGDRESVLAGSVGAGFSYLQLGAESGIDETSFSIFDFTVPVYFSVHPVKWIGIYATP
                     RYTLRLVGGASHWFGGTGGIRLGNRFGGFAEYSLFTSSSATTNLSQVAFGLSFGIGGK
                     "
     assembly_gap    4697..4744
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4745..5489)
                     /locus_tag="PK198_19965"
     CDS             complement(<4745..5489)
                     /locus_tag="PK198_19965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF41083.1"
                     /translation="MTLTQPAILAFTASGAGATCLGTQSGSITIESIQGGTAPFEVWI
                     NGSFTADVDTLPFALPGFAQGNYTVAIRDANQCEIGSTVNVPQPQVYTLDLGLSQTIR
                     LGDSLQLNPMANFVIDSVLWTPLAGVLNPDLPNTFVRPSIPTTYRLIAFDSNGCSAAD
                     SILVLVDRNSRIFVPNAFSPNDDNINDRLTVFSDAGVSTVLSYQIFDRWGNQLFSGGP
                     FPPNDPQFGWDGSFKGRPMDAGVYVFWVEL"
     assembly_gap    5508..5517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5518..>5700)
                     /locus_tag="PK198_19970"
     CDS             complement(<5518..>5700)
                     /locus_tag="PK198_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41084.1"
                     /translation="TLSATGTNPLCNGAATGSIALTVNGANGTPSFDWSVNALDGIQN
                     PTGLTAGTYSVTVTDA"
BASE COUNT         1332 a         1382 c         1475 g         1309 t
ORIGIN      
        1 gggcgcacgg ccattcgcag gcgggcgttc agcaatggtg cattggatgt ttcgggcttg
       61 ccgccgggga tgtatgcggc ggtggtgtat gaaaaaaata cgccggtagc cgttcagaaa
      121 gtagtggtga aataacgcag acaatttcca cggggcccgt ttcgttggat gtgcgttcaa
      181 aaaatttaac ggactatgaa aagttggacg ctcattttta caatgctttt cgcagtttcc
      241 tttgtgtctg cccagcagca ggatacgctt tggaaaaagc atttctctgc ggaagaactt
      301 gcccattttg atggacagga agacaccctg gcgctgttgg cctacgtcat tgtcaacgac
      361 tcgctgccgg aacatcgcta ttctgcctgc cataagttga ttcctatgtt ggtcagcgca
      421 ttaaaaaccc ccaattcttt ccgttttccc ttcgagcgcc tcaagtcggt ttccatcatg
      481 tatccgcccg acagctcgtt ccgggttttt acctggcaac tgtatgtgga caaagacgag
      541 tatcgctatt atggcgctat tcaaatgaat accaaggact tgaagttgta cccgctgaaa
      601 gatcgctctt ttgaaattaa aggcaatctg gaacaattgg aactcacgcc ggagcaatgg
      661 tatggcgtag tttattacaa tatacaacaa accgacgcaa aaaccggacg ctattacctc
      721 ctgttcggct ttgacggtaa tgagtttttc cgcaaacgaa aagtgctgga tgtgcttact
      781 ttcaagaatg gccaacccgt tttcggcgcg ccggtgtttc ggcatgaaaa caaaaaaggc
      841 gaggtcagtt ttcgcaaacg ccgggtcatt gagtattcgg cggcggccag tgtgcgcttc
      901 aattacgacc ccgcactggg catcatcatt tacgatcacc tcatcactat gccgggcgaa
      961 gatggagaag gcccgaacaa ctatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gccgagacaa
     1141 taggtaaaat ctatgcgcag tgaaggggtg actacggggc tatggcctcg gcggagtcgc
     1201 cccttcacta tcctgccaca ctgcaccaac caacatgcac gcacaccatt tcagcgtacc
     1261 gcgcacggct catttctact cgttgggcca acccggccca cacagctccg agtgctgggt
     1321 aatcttgcat ggctacgggc aattggctaa gtatttcctc cgccggtttg atgcagtagc
     1381 cgatgagcat acctgtgtgg cagcccccga agcgctttct cgtttttaca cagatcagga
     1441 gggaatccgc gtgggtgcct cctggatgac aaaagagcag cgactggctg atattgaaga
     1501 caatatgacc tacttagatg gcttatacga tctgttgaaa aaagacttac ccgccgattg
     1561 ccgtttcatt ttgctgggat tttcacaagg ctgcgccacc ctcatgcgct gggtattgcg
     1621 ccgacaaccg ccgttggccg gtctcgttct gtatgctggt tccattcccg acgatctgcc
     1681 ttatgccaca catcataagt atctgaacag ctttcccatt catgcggtgt atggcgatgc
     1741 agacgagtac ctcaacggag agcgactggc ggcttggcga ggcatgttgg acggtatgca
     1801 ctggcaactg cgataccacg tttttgaagg cgcccacgtc gtttcccgta atgtattggc
     1861 acagttaaag caggaactca ataccccgat ctgacaaaaa tcattgattt ttctgttgaa
     1921 agtgcgcaac ttgagcctgc cgggaatatt ccggtaagag accaaaaatc cgaaccatga
     1981 aactgcgcat ccattcattc ttgggatctt gggggcttgt ttcgggcatc cctgctttta
     2041 ttttactgtt gatggcattt gttccttatc aaaggtctgc cccttcagaa agggagatgc
     2101 agactgcttt ttctttaggt tgtctggaac gcgccgacat tgattttata cttacggagc
     2161 ctggagttat cactgccgaa gcagaatggt tgccggccga ttcgcccgcg gcattgctac
     2221 tgtacggacc cggccagatg aactactatt tgcgcaaaga aggcaaaagc ggtgaaaaat
     2281 tagagttcac cgtcacagat cgccacatag cccggggcac ggaatggagt gtggtggtac
     2341 tcaatcaaca ggataccaaa atttccggtc tgttgaaagt ccgtctccct ggtgaggagc
     2401 ctaagtgagg caatgggttg tttataaggt agttagagag agagctgtcg gatttttcat
     2461 cgtccggtaa tctgccggta ttggatcaaa aaatggcatt catacccaac ccaatccagt
     2521 tgccgcgacg gatggcgccc accgtatttt gagcaaaata ggcgttgacg gtagagccgt
     2581 cgcccgcagt cccggcatcg ccgctgtgca gtgtgtagtg cagttgtatc accatatcgc
     2641 gcttgcggag gtagtaattc agacccaaat caatggtttg ttcacgcccg gaagagagtt
     2701 tcagcacgcg ggcgtctgct tggccggcgg catccatagc accttcaaaa cgcatgagca
     2761 tggcggaggg ctggagcata aatttgacca ccgtaatgtt gtacccggcg cgaatatgcc
     2821 ctgtgccgga gcgggcggta aaaatattag cctctccgcg cagaccttgg cgttgcagaa
     2881 acatccactc accgtccaca ttccacctgc cgtagttcag catcagcccc ggcccccagg
     2941 cgacagcgtt ttgaaactgg tcggttttac cttgtctcgc gatgttgagg tctatggaaa
     3001 taccttttct ttcgttgaaa aaattggtgc cgtaagcgat gccgtactgt tccatttcgg
     3061 ggtcgcccaa tgccaatgaa agtctgccgg cgagcaaggg cgagtattga atgcctactg
     3121 agtttccgtt caagccggtg agcaaggggt tgaaaatccc cgcattgtag agcagcgaca
     3181 gtttttcctt tttgatcata ccgcccaggt tgatgcctgc cgctcgccca gatcctcgtc
     3241 cgacaagatg ggtacgcaca taactctgag acatagattt ttcaaaagac gaaacgctcc
     3301 atgcggcagt gatggattct cgttgaaact gcggcctgaa ccagccggcc gtgaggtgca
     3361 gatagtcttg gccaaggggc aattgccatt gaatgaacgc atcccagata ccgaccgagg
     3421 gttccgcgtt attggtagga ccttgcgtgg cggacaataa gtcgtggccg gcctgatcga
     3481 agtgcatggc taaataatat ttaaatccct gataaggctc gccgcgcatg agcaaccgcg
     3541 cacgccggaa ataaacgttc aggcggttgt ccaccgggcg ataggagagg gtagtggcgt
     3601 catacacctc ttgccccagg gtgtagatac tacacaattg cagcatggcc gatgccttca
     3661 gcgctgcgcc gggcttgggt tggagccatt tgatctgacg ggaagagtcg ctttgtgccg
     3721 tggataagta gcaattagcc atcaacaggc ccacgacgag aatgcgtttg gaaatcatgg
     3781 ttggtaatta aaacagggcg aaataacgca gcgggccgga gtctttttgt gatgtatatt
     3841 ccaaaagggg cataaaaaaa caggctgcct cccgcatgcc ctgaaaaagg gaggaaagca
     3901 gcctgttttg caacagcgta aaactatgtg attatttgcc gccgatgcca aatgagaggc
     3961 cgaaagcgac ctggctcaaa ttggtagtag cactgctgct ggtgaacaat gagtattcgg
     4021 caaagccgcc aaagcggttg ccgaggcgaa taccacccgt gccgccaaac cagtggctgg
     4081 cgccgcctac caaacgcaag gtgtagcgcg gagtagcata aatgcctatc cacttgacag
     4141 ggtgtacgga aaagtaaaca ggtacagtga agtcaaagat cgaaaaagaa gtttcgtcaa
     4201 ttccagattc tgcgccgagt tgcaggtagc tgaaacctgc gcctacgcta ccggcgagta
     4261 cagattcgcg gtcgcccaaa aactgatatt ttccatagag gccggaggtg cccagcaagg
     4321 atactttgcc gccaatatcc agtttatcag tcacgccata gcgaaaatct gcttcgagca
     4381 tcggagcaga aacggaggcc gtgtcaagct cctcaatgcc caatacaaag gcatacttca
     4441 cgccgctgcc gccaacgttg acctcagaat tgccctggcc gacagtacgg ccggtttgca
     4501 tggtggacat agaaacacaa ccggagagta gcaaggccgt tactcctgta aatacgaggt
     4561 agtgtagtgt tgagattgtt tttttcatgg ttacaatgtg attttgtttt gaaaaggaaa
     4621 agttttactg ttgcgacggc aaaagtaaac ctcttcccca caattcactc tttttttgcc
     4681 tatctcatca aggtcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnccaact ctacccaaaa tacatacact ccggcatcca tcggtctgcc tttgaaggag
     4801 ccatcccagc cgaattgcgg atcgttgggc gggaaggggc cgcctgaaaa cagttgattg
     4861 ccccagcggt cgaaaatctg atacgatagc accgttgaca ccccggcatc cgaaaacacg
     4921 gtcaggcggt cattgatgtt atcatcgttg ggactaaatg cgttcggtac aaaaatgcgg
     4981 gaattcctat ctaccaacac caatatggag tcggcggcgc tgcatccatt gctgtcaaag
     5041 gcgataagcc ggtaagtagt gggaatggag gggcggacaa atgtatttgg aaggtcggga
     5101 ttcaagaccc cggcgagtgg cgtccaaaga acggagtcaa tgacgaaatt ggccattggg
     5161 ttcagttgca ggctgtcgcc caaacgtatg gtctggctta gccccaaatc gagcgtgtaa
     5221 acctgcggct gcggtacatt caccgttgag ccgatttcgc attggttggc atcgcgaatg
     5281 gctacggtgt aattgccttg tgcaaagccg ggcagcgcaa atggcaaagt atctacatcg
     5341 gctgtaaatg agccgtttat ccatacttca aagggtgctg ttccgccttg tatgctctct
     5401 atggtaatgc tgccgctttg tgtgccgaga caagttgctc cggcgcccga agcggtgaat
     5461 gcgagtattg ccggctgcgt taatgtcacg gaagtattgg cctggcannn nnnnnnnccg
     5521 catcggtcac ggtgacggag taagtgcccg ccgtcaaacc agtcggattt tggatgccgt
     5581 ccaatgcgtt gacgctccag tcaaagctcg gcgttccgtt tgcgccattg acggtcagtg
     5641 cgatgctgcc cgtcgccgcg ccattgcaaa gcgggttggt accggttgcg ctcagcgtga
//
LOCUS       DAORLF010000314         5697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7707_length_5697_cov_2.244062, whole genome shotgun sequence.
ACCESSION   DAORLF010000314 DAORLF010000000
VERSION     DAORLF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7707_length_5697_cov_2.244062"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    242..367
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            541..2091
                     /locus_tag="PK198_19975"
     CDS             541..2091
                     /locus_tag="PK198_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41085.1"
                     /translation="MKKTLLLSFFLVFALLQTLAQPKHDCYWMMGYSDSSPDSSIWGA
                     SVMDFCGDSLQIYEINRPMNFDFTNASMCDSAGNLLFYSNGIYIANRLHQPMLNGTGL
                     LTPGTSGFNEDGIPIQQGVLILPWPGQTQKYFVFYGRLGYFYYNNVLTAGNVSLYYAV
                     VDMSMNNGLGAVTHKNVLITNDTLKMGRLSAVRHANGRDWWLVAGKFHNPELLRFLVT
                     PDGVMNYGSVPDENFVRKGIGQVVFSPDGNYFVDVTLQNWGDNYVSFFEFDRCTGDFF
                     SRGRFGYDDVIAHAGVAISPNSRYAYQTSTYLIRRFDLWADDIPGSMDTAALYDGYEE
                     PIPGFDSLTLPTVFYMAQLAPNGKIYVVSTNTVRSVHVIERPDEEECDVRQHSVIFPK
                     LTRTMPNFPNFRLGALAGSPCDTLGATAVQEPVPGRGIQAYLLPNPARDYFVLALDTQ
                     NAALPADAPLRLHLSNSMGQTLRQYQLTAAQREHRISTEELPAGLYYVSIMYGGHLLK
                     TEKLVVVR"
     gene            2469..3911
                     /locus_tag="PK198_19980"
     CDS             2469..3911
                     /locus_tag="PK198_19980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41086.1"
                     /translation="MQLDDRLYSTDHAETGTAMMNFCGDTLSITPTPGQPFSFLSTIA
                     VISDDEGRLLFYTNGFQIRNAAHELMLNGDSLQVEDVLELRNIGLDMIQGAVIVPFPE
                     QPGKYVLIHAESFLGEGYLVATPLKYTIVDMSLDGGLGGVVEKNVVIRNDTVEYGKLV
                     ATRHANGRDWWVVASKFDDGRLIRLLITPNGVEDYGLTDEAYVSYTDYGNAAFSPDGS
                     YYTNTSVTLVSPNDISIDLLRFDRCTGAFSTIENYIDNTATGYSFVAFSPNSQYMYQS
                     YGRVINQFDLSVIPLAQGRVEVAQYDGFQYTQPGFDPVQVYFFQPELGPDGRIYISPS
                     SPSPYFHVIESPNSPGLACNVMQHALRLPFVNAGVPNMPNYRLGALAGSPCDTLIMNS
                     VQQPAKDLAPLRVFPNPAQTHFTIASSSPATLLRLYDGMGRQVLVQALAAGQPEHRIA
                     LPAGLPAGVYVAVAEGREGILGRARVVVAR"
     gene            complement(3929..5611)
                     /locus_tag="PK198_19985"
     CDS             complement(3929..5611)
                     /locus_tag="PK198_19985"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRF41087.1"
                     /translation="MGLVLYDLGFEHTVWLSAGYAIIVIPLLLLNLRLHFTEKKVPRL
                     AWASSIALASLMLAVLIFVVSGNSWADSVEQLHNYHLAGIALYFVHRASLTIQGLLSR
                     QIHPANLFVGSFVAIIVLGALLLLLPAATPGSISVTDAFFTATSAVCVTGLIVVDTAK
                     DFTFFGQTVILFLIQLGGLGILTFTSFFAYFFKGNSSLQETVLLRDMVSSEQLGNVFK
                     VVVKIVVFTLVIELIGALGIYFSTDAAHFESWYERVFFSLFHSVSAFCNAGFSILTDG
                     LYDSRFRFHYGLQWMVILLVIFGGLGFNILNNLYEYLKITIKNSIYTWERNLDAVVVR
                     PRIISLNSRIVIQTTLILLIAGLVFFLISEFRHSIAQHNSWWGKLTTAMFCSTTPRTA
                     GFNTIDMRSLSVPGVMATMLLMWIGASPASTGGGIKTSTFALAVMNVWAVVRGRPHIE
                     LGHREVPTESVQRAFAIIGLSFVALSVGIFALSWMEPDKDLVDLSFECVSAYSTSGLS
                     LGITSSLGTGSKWVLIVLMFTGRVGTLNLLTGMLQQIHRAHYRYPQESVLIN"
BASE COUNT         1387 a         1492 c         1406 g         1286 t
ORIGIN      
        1 aaatgatcac tttatttttt tatcgcccct tattggatat tttttcaaaa aacccaataa
       61 tcaacttgcc cttttgaaat aatgcaaata tattgcgccc cgataacttt tcttcacacg
      121 tttaaattct ttacctatga aaaacagaat tttattcctc aaatacctcg atttgtcagt
      181 tcttgaaatc ttggttgatc gttaggaatt gcgtggcgta ttccgcagcc tgattccaga
      241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnttg aaaatctacg tttagtgaag gggtgacttg cccggttaat gcactgatag
      421 tcaccctttc actacgcttg aaaatctacg tttagtgaag gggtgacttg cccggttaat
      481 gcacttatag tcaccccttc actaccctct ttctttatca cacaacacat atttgctccc
      541 atgaaaaaaa cacttctact ctcgtttttt ctggtatttg ctttactcca aaccctggcc
      601 caacccaaac acgactgcta ctggatgatg ggctatagcg actcctcccc agacagctcc
      661 atttggggcg cctctgtcat ggacttttgc ggcgactctt tgcagatata cgaaataaac
      721 cgccccatga atttcgattt caccaatgcc tctatgtgcg actcggcggg caatctcctg
      781 ttttactcca acggtatcta catcgccaac cgcctgcacc agcccatgct caacggtacg
      841 ggcctgctga cgcccggcac ttcagggttt aatgaggatg gcattcccat tcagcagggg
      901 gtacttatac tcccctggcc tgggcaaaca caaaaatatt tcgtctttta tggaaggctg
      961 gggtactttt attacaacaa tgtactgact gcgggcaatg tatccctcta ttacgctgtc
     1021 gttgatatgt ccatgaacaa tggtttgggc gccgtaacgc ataaaaatgt actgataacc
     1081 aacgacacct tgaaaatggg gcgcctttct gccgtacgcc acgccaacgg cagggattgg
     1141 tggttagtag cgggtaaatt tcacaacccg gaattgcttc ggtttctcgt cacacccgat
     1201 ggggtcatga actacggaag tgtgccggac gaaaattttg tacgaaaagg aatcggacag
     1261 gtagtctttt caccggacgg caactatttc gtggatgtca cattacaaaa ttggggagat
     1321 aattatgttt ctttttttga gtttgaccga tgtaccgggg attttttctc cagagggcgt
     1381 tttggatatg atgatgtcat agcccatgcc ggagtagcga tatcgcccaa ttccagatat
     1441 gcctaccaga cttccactta cctgatacgg cgcttcgacc tctgggccga cgacatcccc
     1501 ggctctatgg atacggcggc tctgtacgac ggctacgagg agcctattcc agggttcgat
     1561 agccttactc tgcctactgt tttctacatg gcgcaattgg cgcccaatgg aaaaatttat
     1621 gttgtttcta ccaataccgt tcgttcggta catgtcatcg aacgccccga cgaagaggag
     1681 tgcgacgtgc ggcaacatag cgtcatcttc ccaaaactga cccgcactat gcccaacttc
     1741 cccaacttcc gcctcggcgc gcttgccggc agcccctgcg atacactggg cgccaccgcc
     1801 gtgcaggagc cggtacccgg acggggcata caggcgtatc tcttgcccaa tcctgcgcga
     1861 gactacttcg tgctggcctt ggatacccaa aatgccgccc tccctgccga tgccccgctg
     1921 cgcctgcatc tgagcaacag catgggccaa accttgcgtc aataccaact gactgccgct
     1981 cagcgcgaac accgcatcag caccgaggag cttcctgccg gcttgtatta tgtgagtatt
     2041 atgtatggcg ggcatttgtt gaagacggag aagttggttg ttgtcaggta gtggcggctg
     2101 ctggctgctg gcctcaagct gctggccggc ccgttggatg ttttcaggtg acatctaacc
     2161 ccgagcctta gcgaggcggg aaggtgtcgc atgaaaacat ccggagatgt tcccttcaca
     2221 gccgcgagat gacaccttcc gccgccaaga ggctctcttc cattgtaggg gccggggagg
     2281 cgacggcaga tgtcacctcg ctcgtaagtg caagccgtta gcagacctgt agcttgtagc
     2341 ctgaaaaaaa ctgataaaca gcaacaacaa aatcatcatc ttatgaacac aaaatcacct
     2401 ttccgacgct tgtttttatt actgagctgc ctgtgggcag gtacgctcgc tgcacagccc
     2461 ggccccgatt gcaactggat gaccggcttt attccaccga tcatgcagaa acgggtacgg
     2521 ccatgatgaa tttctgcggc gacacactgt ccatcactcc cacgcccggc caacccttca
     2581 gcttcctcag tacgatagcc gtcatcagcg acgacgaggg gcggttgttg ttttacacca
     2641 atggcttcca aatccgaaat gcggcgcatg aactgatgct gaacggcgac agcctgcaag
     2701 tagaggatgt actcgaactg agaaacatcg gcttagacat gattcagggc gcagtgattg
     2761 tacctttccc cgaacagccc ggaaagtatg tgctcattca tgcggaatct tttctgggag
     2821 aagggtatct ggtggccacg cctctgaaat acaccattgt ggatatgtcg ctggatggag
     2881 gcctgggcgg cgtggtggag aaaaatgtcg ttatcagaaa cgacacggtc gaatacggaa
     2941 aattggtggc cacacgccat gccaacggcc gcgattggtg ggtcgtagcc tccaaatttg
     3001 acgacggacg gctcatccgg ctactcatca ctcctaatgg tgtggaagac tacggcctta
     3061 cggacgaagc ttatgtttca tatacagact acggcaatgc agcgttttcg cccgacggca
     3121 gttattatac caacacaagt gttacccttg tcagccccaa cgatatttca attgacctgt
     3181 tgcggtttga tcgctgtaca ggagcgtttt ccacgattga aaactatatt gacaatacgg
     3241 caacagggta cagctttgta gcgttttcgc ccaattcgca atacatgtat cagtcttacg
     3301 ggcgggttat caatcagttc gatctctcgg taatacctct ggcgcaaggt cgggttgagg
     3361 tagcccaata cgacggattt caatacacgc agccgggttt tgatcctgtt caggtgtact
     3421 tcttccaacc ggaattaggc ccagacggca gaatatacat ttcgcccagc agcccctcgc
     3481 cgtattttca cgtcattgaa tcgcccaatt cgcccggctt agcctgtaat gtcatgcaac
     3541 atgctctgcg cttgcctttt gtcaatgccg gagtacccaa tatgcccaat tatcgcctcg
     3601 gcgcgcttgc cggcagtccc tgcgatacgc tgataatgaa ctcggtacag caacctgcaa
     3661 aggatttggc tccgctccgc gtcttcccca atcccgccca aacgcacttc accatcgcct
     3721 cctcctcgcc cgccacactc ctgcgcctgt acgacggaat ggggcggcag gtacttgtgc
     3781 aggcactcgc ggccgggcag ccggaacatc gcattgcgct gccggcgggc cttccggcgg
     3841 gtgtgtatgt ggcggtggca gaggggcgcg aagggatatt gggcagggcg cgggtggtgg
     3901 tggcgaggta atgccttgaa atgttgcgtc agttaatcaa caccgactcc tgcgggtagc
     3961 gataatgcgc ccggtggatt tgctgcaaca tgcccgtcag caggttgagc gtgcccaccc
     4021 ggccggtaaa catgagtaca atgagtaccc acttgctgcc cgtgccgagg cttgaggtga
     4081 tgcccaggct gaggccggag gtggaatagg ccgacacgca ctcgaaactc aggtccacta
     4141 agtctttgtc aggctccatc caactgagcg caaaaatacc cacgctcagt gccacaaaag
     4201 acaacccgat gatggcaaag gcccgttgca ccgattcagt cggcacttcc cggtggccga
     4261 gttcgatgtg aggccggcca cgcaccactg cccaaacgtt catcaccgcc agggcaaaag
     4321 tactggtttt gataccgccg ccggtggacg caggcgaagc gcctatccac atcagcagca
     4381 tggtagccat cacgccgggc acactgagac tgcgcatgtc tatggtgttg aacccggccg
     4441 tgcgcggcgt ggtactgcaa aacatggcgg tggtgagttt gccccaccag gaattgtgct
     4501 gagcaatgga atgccggaat tcggagatca gaaaaaaaac aaggccggct atcagtaaaa
     4561 tcagggtggt ttgaatcaca atgcgggaat tgagcgaaat gatgcgcggg cgaacgacaa
     4621 cggcatcgag gttgcgctcc caggtataaa tgctgttttt gatggtaatt ttgagatatt
     4681 cgtagaggtt gttgaggata ttaaaaccca aaccgccgaa aatgacgagt aaaatgacca
     4741 tccattgcag gccgtaatgg aatcggaacc ggctgtcgta caggccatcg gtcaggatgg
     4801 aaaaacctgc gttgcaaaac gccgacacgg agtgaaacag ggaaaaaaac acccgctcgt
     4861 accaggattc gaagtgcgcc gcatcggtag aaaaatagat gcccaacgcg ccgatcaact
     4921 caatgacgag agtaaacacc acaattttga cgaccacctt gaacacattg cccaactgct
     4981 cggaactcac catgtcgcgc agcagcaccg tttcctgcaa agaggaattg cctttgaaaa
     5041 aataagcaaa aaaggaggta aacgtgagta tgcccagacc gcccaattgt attaaaaaca
     5101 aaatgacggt ctgaccgaaa aaggtaaaat ctttggcggt gtccaccacg atcaggcccg
     5161 taacgcatac ggcgctggtg gcggtaaaaa acgcatccgt gacgctgatg gaacctggcg
     5221 tggcagcagg cagcaacaac agcaaagcac ccaaaacgat aatggctaca aagctgccca
     5281 caaacagatt ggccggatgt atttgccggg aaagcaaacc ttgtatcgtc aatgaagcac
     5341 ggtgtacaaa atacagcgca atgcctgcca gatggtaatt gtgcagttgc tcaaccgagt
     5401 cggcccagga attgccggat acgacgaaaa taagtacagc cagcatcaac gaggccagtg
     5461 caatggacga tgcccaggcc aggcgcggta cttttttttc tgtaaaatgc agccggagat
     5521 tgagcaacag caatggaata acgatgatag cataaccggc gctgagccaa accgtgtgct
     5581 caaaacccag atcgtacagg accagaccca aggctatggt ttggaggaag tatgctacga
     5641 acttcacccg cttcccgaca aaaagtcaga gatcaatcct cgttgttttt tatcaac
//
LOCUS       DAORLF010000315         5680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7741_length_5680_cov_1.844978, whole genome shotgun sequence.
ACCESSION   DAORLF010000315 DAORLF010000000
VERSION     DAORLF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_5680_cov_1.844978"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK198_19990"
     CDS             complement(<1..406)
                     /locus_tag="PK198_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRF41088.1"
                     /translation="MKSTLNRMAMKPVFLLLLLVFSVALHAQLTIVLGSIPANTPPGS
                     DIYIAGNFNNWNPGSAAHKLILNQQGQYSITLNIAPQSIQFKFTRGSWPTVEGNASGG
                     FIPNRSYNYTGGAQTLALSIAGWEGLSGSTGTA"
     gene            complement(634..1908)
                     /gene="fahA"
                     /locus_tag="PK198_19995"
     CDS             complement(634..1908)
                     /gene="fahA"
                     /locus_tag="PK198_19995"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRF41089.1"
                     /translation="MTIRANDPSLRSWVDVPSGSDFPIQNLPFGIARRKSGDAPGVVV
                     AIGEQIADLRVLAEHGFFDDLDIPRAVFSQPVLNDLIALGKAKTSALRNRISQLLDSD
                     FDEWDASELAHFFLFPQTEVEMLMPLRVGDYTDFYSSIEHASNVGAMFRDPQNPLLPN
                     WRHLPVGYHGRASSIVVSGTPIRRPKGQTLPVGADVPVFGPSARMDFELEMAFVVGKN
                     TQLGESVPVADAEDYIFGLMLFNDWSARDIQQWEYVPLGPFLGKNFGSTVSPWVVMLE
                     ALEPFRVAGPKQEPEVLPYLQTEGPGNFDLHLEVWIRPEKGEAARVCLSNFKYMYWNM
                     AQQLAHHTVNGCNIQVGDMCASGTISGPDAGSYGSMLELSWKGTRPIALPDGSTRTFL
                     NDGDEVILRGWGERNGVRVGFGEARGALLSAI"
     gene            complement(1934..>5369)
                     /locus_tag="PK198_20000"
     CDS             complement(1934..>5369)
                     /locus_tag="PK198_20000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41090.1"
                     /translation="GYSFLWQNGNQTPNITVAAGAVPGALDAYGITVTDAAGCSVSDQ
                     VLVRTTNSIDSLQRAVTHVSSCGGADGQIFLQPLNGLPPFSYQWSSTNGVTGSANGIP
                     GAYTITGLPQGVYRVTVTDGSNPPCPFILRQVFVNGPDAVVLPPTITPVTCRGANNGA
                     ICLNVSGDAPQYLWEGGATSACISGLSGGMYSVTITSGNCQTVLNDLFVPEPDSIKII
                     PQFQMPSCHNSSNGAIRLSVFGGSPGYQYAWSHGANSRDATGLAAGVYTVTVTDSRGC
                     VAVQTYTLAAPAPLQVQIDSLQSPSCFGYNNGLIRVSGAGGTGPYHYAWPNGASSPVL
                     FQTPSGFHRVTVTDLNGCQQLRNIFLPEPAPLALSTLGVVQPECRGSNDGLISVSGSG
                     GTEPYTYIWSNGMFTPQISGLEPGSYSVTMTDARQCPAIVRHFELNSLSQLQLGIATT
                     APSCVGQTNGSISLTPSGTGPFSLVWQDSGSQRPNRAQLGVGNYQVNILDGRGCSYDT
                     SIVLTAVQVFDVQKNILPPACFNGLDGVIDVTLTQAGQQPYNFLWSNGSHDEDLIGVP
                     AGDYALTITDALGCRYVSPLIELPNPEPLEMTVIGMGTVLCHDDSSGYIEVVIDGGTA
                     PYSNPSLYDIPTGSYFLQATDARGCPVDTTIVLPAPPPLNVQIALTQSGDCQSLIVTR
                     LQATVTGGIQPYRYVWSNGDTTAIIMNAAPGDYALTVTDANNCSKVITAIKVREQIQP
                     LALVSFAATPLTCFGAQNGRLKVRISGGSSMYRYHFSNNQIITTTADSAEISGLNPGS
                     NYRVTVTDLQTGCTKVAGPLSITSPAPLSYVRTVIIDELCAGSNNGAVMAGTFGGTPP
                     YVYRWTDEAGNLVGTEEDLTGVGPGIYSGLATDANGCTAVLPQQPVSQMYEPLVEVDT
                     AFAISGPLCSGTGFVRVGVEGGAPPYRFDWSNGSSGDSIGGLAPGEYYVTVTDAVDCE
                     LVLGPFHITTSPGISVGINATPPDSTGGNGAVTAIVAGGTAPFTYFWSTGDTTSSLHQ
                     LPAGGYFLTVTDAAGCIGTAGAILVHTAEPEPGAALRVYPNPGKGLVYVEYESPVGIE
                     LIRVRSLGGQLALMHRCSGQRSGTEALQLESLPAGAYVLEIIGGGAILQRRLLMRN"
     assembly_gap    5370..5397
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5398..>5680)
                     /locus_tag="PK198_20005"
     CDS             complement(<5398..>5680)
                     /locus_tag="PK198_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41091.1"
                     /translation="TLNLTEVPVNDLRLTGAAVTYHSGTPATVSNQLASLMIQPVQNQ
                     RIYFLATAQGGCSDEGYFDVMMRPVPSLSFSPADSFSLCKDGIQLVTATA"
BASE COUNT         1269 a         1604 c         1592 g         1187 t
ORIGIN      
        1 ccgccgtacc cgtactgccg ctcagacctt cccagccggc gatgctcagc gcaagcgttt
       61 gcgccccgcc cgtgtagttg tagctgcggt tgggaatgaa ccctcccgat gcgttgccct
      121 ccacggtggg ccagctaccc cgcgtgaatt tgaattgtat gctctgcggc gcaatgttga
      181 gcgtgatgct gtactggcct tgttgattga gtatcagctt gtgagcggcg ctgccggggt
      241 tccagttatt gaaattgccg gcaatgtaaa tatcgctgcc gggcggcgtg ttggcaggta
      301 tagaccccag aacaatagtg agttgggcgt gcagcgcaac tgaaaagacg agcagaagga
      361 ggagaaaaac aggtttcatc gccatccgat taagtgtaga tttcattgtt ttttttgttg
      421 taatgttact attcggccgg tcccccaaag ctacgagcta tgaccacgcc ccaggcgtgg
      481 tatgagtttg tttattcgat ggaaaatcag attttccatt tggggaaatt tcaaaatctc
      541 atcttgaaaa aggccctttt tcattttggg agatcaaact cgtgactcga agctcgcagc
      601 tttttgcagg gcgactgtat agttgcgttg taattaaata gcagacaaga gcgcgccacg
      661 cgcctctcca aaacccaccc gaacgccatt ccgctcgccc cagccgcgca ggatgacctc
      721 gtcgccgtcg ttcagaaaag tacgggtgga gccgtcgggc agggcaatgg gccgggtgcc
      781 tttccacgac agttccagca tggagccgta actgcccgca tcggggccgc tgatggtgcc
      841 cgacgcgcac atatcgccca cctgaatgtt gcagccgttg acggtgtggt gcgccagttg
      901 ctgtgccatg ttccagtaca tgtatttaaa attggacaga cacacgcggg ccgcttcgcc
      961 tttttcaggc cgaatccaca cctccagatg cagatcgaaa ttgccggggc cttcggtctg
     1021 caaatacggc agcacttccg gttcctgttt cgggccggcc accctgaacg gctccagggc
     1081 ctccagcatg accacccagg gcgatacggt agagccgaag tttttgccca aaaacggccc
     1141 cagcggcaca tactcccatt gctgaatatc gcgggccgac cagtcgttga acagcatcag
     1201 accaaaaatg taatcctctg catcggccac cggcaccgac tcgcccaact gagtattttt
     1261 gcccaccaca aaggccattt ccaactcaaa atccatgcgg gcgctggggc cgaaaacggg
     1321 cacatcggcg ccgacgggca aagtctgacc tttgggacgg cgtataggcg tgcccgaaac
     1381 gactatgctg gaagcccgcc cgtgatatcc tacgggaagg tgccgccagt tgggcaacag
     1441 cgggttttga gggtcgcgga acatggcgcc cacattggag gcgtgttcta tgctggagta
     1501 aaaatctgtg taatcgccta cgcggagggg catcaacatt tctacttcag tctgcggaaa
     1561 aagaaaaaaa tgggccaatt cgctggcatc ccattcatca aagtcggaat ccaacaactg
     1621 agatatccgg ttgcgcaggg cgctggtttt ggctttgccc aaggcaatca gatcgttgag
     1681 tacgggttgt gaaaacacag ctcttggtat gtccaagtcg tcaaaaaaac cgtgttcggc
     1741 cagtacccga agatcggcga tctgctctcc gatggctacc accacgccgg gtgcatcgcc
     1801 tgatttgcgc cgggctatgc cgaaagggag gttttgaata ggaaaatcac tgccggaggg
     1861 tacatccacc caggaacgaa gcgaagggtc gttggctctg atcgtcattt ttattgtttg
     1921 tttaaagtgt attctaattc ctcatcaaca aacgccgctg caagatcgcg ccgccgccga
     1981 tgatttccaa aacataggcc ccggccggca aggattccaa ctgcaatgcc tctgtaccgc
     2041 tgcgctgccc ggaacacctg tgcataagcg ccaactgccc gccgaggctg cgcaccctga
     2101 tgagttcgat gcccaccggc gactcgtact ccacatatac caaccctttg cctggattgg
     2161 gatacacccg cagcgccgct cccggctcag gctctgccgt atgcaccaaa atagcgccgg
     2221 cagtaccgat acatccggca gcatccgtca cggtgagaaa gtaaccgccc gccggcaatt
     2281 gatgaagcga ggaggtcgta tcgccggtgc tccaaaagta agtaaacgga gcagtaccgc
     2341 cggcaacaat ggccgttacg gcgccgttgc cccctgtgct gtcgggcggc gtggcgttga
     2401 ttcccaccga aatacccggc gacgtagtga tatgaaacgg tccaagaacc aattcgcaat
     2461 ccactgcatc tgtgacggta acgtaatact cgcccggcgc aaggcctccg atggaatccc
     2521 cggatgagcc gttgctccaa tcgaaacggt acggcggcgc tccgccttct acgcctaccc
     2581 gaacaaaacc cgtgcccgaa caaagcggcc cggaaatggc aaatgccgtg tccacttcca
     2641 ccaatggctc atacatctgc gaaaccggct gctgtggcaa aacggccgta caaccattgg
     2701 catccgtagc caggccgctg tatatgccag gccctacgcc cgtgaggtct tcctctgtac
     2761 cgacaaggtt gcctgcctcg tccgtccagc gataaacata aggcggcgtt cctccaaaag
     2821 tacccgccat caccgcgccg ttgttgctgc ctgcacaaag ctcgtctatg atgacggtgc
     2881 gaacgtagga aagcggcgcg ggggaggtaa tgctcagcgg cccggccact ttggtgcaac
     2941 ccgtctgcag gtcggtgacg gtgacgcgat aattgctacc cggattcagc ccggaaatct
     3001 cggcgctgtc ggcggtagtg gtaatgatct ggttgttgct gaaatgatag cggtacatag
     3061 aactgccgcc cgaaatgcgc actttgagcc gcccgttctg cgctccaaaa caagtgaggg
     3121 gcgttgccgc aaacgaaacc aaagcgagcg gctgaatttg ctcgcgcacc ttgatggcgg
     3181 taattacttt ggaacaatta ttggcatcgg tgacggtgag ggcgtagtct cccggcgcgg
     3241 cgttcatgat gatggccgtg gtgtcgccat tgctccacac atagcggtaa ggttgaatgc
     3301 cgcctgtgac cgttgcctgc aagcgcgtaa cgatgagcga ctgacaatca ccactttgtg
     3361 tcaatgcgat ttgcacattc aaaggcggcg gcgcgggcag tacgatggta gtatccaccg
     3421 gacaacctct ggcgtcggtg gcctgcaaaa aatagctgcc cgtaggaatg tcgtacaaac
     3481 tggggttgct gtacggcgcc gttccgccat caataacaac ctctatatag ccgctcgaat
     3541 catcgtgaca cagcaccgta cccattccga taacggtcat ttccaaaggc tccggattgg
     3601 gcagttctat aaggggcgaa acgtaacggc atcctaaggc atcggtgatg gtcagcgcat
     3661 aatcgcctgc cggcactcct atgaggtctt catcatggct gccgttgctc caaaggaagt
     3721 tgtagggctg ctgcccggcc tgcgtcaggg tcacatctat aaccccgtcc aagccgttga
     3781 aacaagccgg aggtagtata tttttctgaa catcaaatac ttgcactgcc gtcagcacaa
     3841 tgctggtatc gtaagaacag cccctgccat caaggatatt gacctgataa ttacccacgc
     3901 ccaactgcgc tctgttcggc ctttgactgc cgctatcttg ccaaacaagc gaaaacggcc
     3961 cggttcccga aggcgtcaat gagatgctgc cgttggtctg cccgacacaa gacggcgccg
     4021 tcgttgctat ccccaattgt agctgcgaca gggagttcag ctcaaaatgg cgcacaatag
     4081 ccgggcattg ccgggcatcg gtcatggtaa cggaatagct gccgggttcc aaaccggaaa
     4141 tctgcggagt gaacattccg ttgctccaaa tataggtgta aggctccgtt ccgccgctgc
     4201 ccgataccga gataaggcca tcgttgctgc cccggcactc cggttgcacc acgcccaatg
     4261 tactgagcgc gagcggcgcg ggttcgggca gaaaaatgtt tctcaactgt tggcagccgt
     4321 tcagatcggt gacggtcacg cgatgaaaac ctgaaggcgt ttggaacaaa accggcgagg
     4381 atgccccgtt tggccaggca taatgatatg gcccggtacc gccggctccg cttacgcgaa
     4441 taaggccgtt gttgtacccg aaacaggacg ggctttgcag cgaatctatc tgcacttgca
     4501 agggggcggg cgcggctaag gtataagttt gtaccgccac acagccccgc gagtccgtca
     4561 cagttacggt ataaacgccg gcagccaatc ccgttgcgtc gcggctgttg gcgccgtggc
     4621 tccaggcata ttgatagccg gggctgcctc caaaaacgga aaggcgtatg gcgccgttgc
     4681 tgctgttgtg gcaagacggc atctggaatt gaggtatgat tttgatggaa tcgggttcgg
     4741 gaacgaagag gtcgttcaaa accgtctggc aatttccgga ggtgatggtg accgagtaca
     4801 ttccgcctga aagccctgaa atgcaagcac ttgtagcgcc gccttcccac aaatactgcg
     4861 gagcgtcgcc gctcacattc agacaaattg ccccgttgtt cgcgccccgg caagtcaccg
     4921 gggtgatcgt cggaggcaac accaccgcgt cggggccgtt gacaaacacc tgccgcagga
     4981 tgaaggggca gggcgggttg gaaccatccg tcaccgtcac ccgataaacg ccttgcggca
     5041 aaccggtaat ggtataagcg ccgggaatcc cgtttgcgct gcccgtcacg ccgttggtgc
     5101 tgctccattg gtagctgaag ggcggcaaac cgttgagtgg ttgtaaaaat atttgcccgt
     5161 cggcgccgcc gcaactgctc acatgcgtca cggcgcgttg tagcgagtca atgctgttgg
     5221 tggttcttac caatacctga tcgctgaccg agcagcccgc cgcgtcggtc acggtgattc
     5281 cgtaagcgtc caaagcgccc ggtacagccc ccgccgccac ggtgatgttg ggggtttgat
     5341 tgccgttttg ccacaaaaag ctgtacccgn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     5401 gtagccgtca ccaactgaat gccgtcttta caaagagaaa aagagtcggc ggggctgaaa
     5461 ctcaaagacg gcacaggccg catcattaca tcaaaatagc cctcgtcgga gcagccgccc
     5521 tgagctgtgg cgagaaaata aatccgttga ttttgaacag gttgtatcat taaagacgcc
     5581 aattggttgg aaactgtggc gggtgttccc gaatgatacg tcacagcggc gccggtaagt
     5641 cggaggtcat tgacgggcac ctcggtcagg ttcagggttt
//
LOCUS       DAORLF010000316         5651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7792_length_5651_cov_2.062187, whole genome shotgun sequence.
ACCESSION   DAORLF010000316 DAORLF010000000
VERSION     DAORLF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_5651_cov_2.062187"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..2134
                     /locus_tag="PK198_20010"
     CDS             35..2134
                     /locus_tag="PK198_20010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF41092.1"
                     /translation="MALIGQIRKNSWILIVMMALASLGVIIMYMSDDRTSLFGGSQFT
                     MGKVNGEKIDWRNFSRVEDVMYRNSSADRRAYLWNYFAEEVLIKKEAEALGLGVSVTE
                     LKALEFGPAYSPVIQQRFADPATRQVDVEQLNQFKTAIEGGNLTDPQARAFWAHQEKE
                     IIKDRLQTKLGGMVAKALYTPTWMAELSYTDLNQRFDMAYVQVPFDELDNTEVSLSDD
                     DYAAYLKENAAIYKSEKETRALQYVAFTVAPSAEDSATYQAKIAGYVPEFAAAENDSN
                     FVEQRFGIIDAAYFKKDDISEVIADTVFSMAPGSVYGPYLDNGAYMAVKVLDRKVIPD
                     SVRSRHILIQAANPMELATAQARVDSLKGVIESGAATFEALAAQFGSDATKDKGGDLD
                     FAAPGGMVKPFNDLIFFEAEQGKLYSVVTQFGVHLVEVTAKKFINNNTGVKLAYLMER
                     IMPSDKTQGNVKDKAIALMSGNRKLEDLVAAAKKDKSLSLAVTPLLEANDYNIADLGS
                     GQSGRDMVRWAFTAKKGEVSPEVFSFQNAELLTVDKFVLAGLKTIQPAGLPSVENIRE
                     EIKQLVLNKKKGEVLADRMKGMDLQALASKFKTEIDTAQSISFGSGFLPDLGAEPKVI
                     ATAAALALNKTSKPIVGSSGVFVVQVYNKPEMAPAADYTATKTSLSSPSRAQIPSMIM
                     QALMKNANIKDSRSKFF"
     gene            2244..3341
                     /locus_tag="PK198_20015"
     CDS             2244..3341
                     /locus_tag="PK198_20015"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRF41093.1"
                     /translation="MKYTAIGLMSGSSLDGLDIAACTFEMDGDKVVGWALLQGETQPF
                     PEAWQERLRRLPESDARSLASAHSELGRWMGQCVADFMQRHALRPDLVASHGHTIFHF
                     PAERFTTQIGDGAAIAAACGCPVVCDFRTGDMALGGQGAPLAPLADRMLFPDYDVLLN
                     VGGIANITCKLGSRYVACDVGGANQILNALAQTLGLPYDDGGRLAASGQYIPALAEAM
                     DALPYFSWPPPKSLGNDWVQAHQTQAYLNWPASVEDRLHTACRQLARQTAGAIQRIFE
                     SEKQSRPAYRMLATGGGAYNTFLANCIVEECTSLMGECVLELPDAGIVNYKEAVLMAL
                     MGVLRVLEIPNCIASATGAERDAVGGAVYLG"
     gene            3355..>4332
                     /locus_tag="PK198_20020"
     CDS             3355..>4332
                     /locus_tag="PK198_20020"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF41094.1"
                     /translation="MDKNKLIFVTGGTGFLGSYLLRYLVKEGYTHIRALRRSGSSMDL
                     VQDVAGRIEWIEGDVLNPFSLEEGMSGAAQVYHCAAAVSYQAADKARIMSVNVEGTAN
                     VVNEALEAGIEKMVFAGSVAALGQPKEGFKTTETTPWEWKKELSSYAVSKRLAELEVW
                     RGAAEGLNVCVINPSVILGAGRWDDGPLKIFSLVHRNFPICPPGRSGFVDVRDVAQSM
                     MRIMESDISGERFIVSAENMYFQELMALMAQALDKKAPTIKAGNALMQLARIGDAVGA
                     LLTGRPAEITRAAIYHSSLLSEYDNSKLVQALGYSYRPISETIREAGALF"
     assembly_gap    4333..4342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4397..>4727)
                     /locus_tag="PK198_20025"
     CDS             complement(4397..>4727)
                     /locus_tag="PK198_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRF41095.1"
                     /translation="KGYNLSVDHSKLGYDICAFLGIYLDKSSLYDDAAERLQEIPEVV
                     SLHYTTGLYNIFAKILCRDTNHLREVLHDKIQKIPGIQRTETFISLEEGVDRPINLDS
                     DPFEEEA"
     assembly_gap    4728..4891
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5075..>5651
                     /locus_tag="PK198_20030"
     CDS             5075..>5651
                     /locus_tag="PK198_20030"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41096.1"
                     /translation="MLKRLPSGESSFEKIQTGKQLYVDKTRQIYELLSSGDFFFLSRP
                     RRFGKSLTVSTLKALFEGRRELFEGLWIAEHGGWDWSKTNPVISLSFNGLDYKTHGLE
                     KALTLELDKIAAANGLPQSGLSPKDKLRELIVGLSQRGPVVILIDEYDKPINDYLTDY
                     EMADTNRDVLGNFYGALKANDITDNLRFVFIT"
BASE COUNT         1328 a         1389 c         1504 g         1256 t
ORIGIN      
        1 gactatcaat acattgaatt aaaagtacag tagtatggcc ttaattggac agatccggaa
       61 aaactcctgg attttgattg tgatgatggc attggcctct ttgggggtta tcatcatgta
      121 catgtcggac gacagaacca gccttttcgg cggctctcaa ttcacgatgg gcaaggtaaa
      181 cggtgaaaaa attgactggc gcaatttctc ccgcgtagaa gatgtgatgt accgcaactc
      241 ttccgccgac cgtcgcgcct atttgtggaa ctacttcgcc gaggaggtgc tcatcaaaaa
      301 agaagcggaa gcgctcggtt tgggcgttag tgttacggag ctgaaagcat tggagttcgg
      361 cccggcctac agcccggtca ttcagcagcg ctttgccgac ccggctaccc gccaggtgga
      421 cgtggagcag ttgaaccaat tcaaaaccgc catcgaaggc ggcaatctga ccgacccgca
      481 ggcccgtgct ttctgggcgc atcaggaaaa ggagatcatc aaagaccgcc tgcaaaccaa
      541 gctcggcggc atggtggcca aagcgctcta tacacctacc tggatggccg aattgagcta
      601 taccgacctc aatcagcgct tcgatatggc ttatgtgcag gtgccgtttg atgagttgga
      661 caatacagaa gtgtctttgt ctgacgatga ctatgcggcc tatctcaaag aaaacgccgc
      721 tatatacaag agcgaaaagg agacgcgcgc tttgcagtat gttgccttca cggtagctcc
      781 cagcgccgaa gactctgcca catatcaggc gaaaattgcc ggttatgtgc cggagttcgc
      841 agcagctgaa aacgactcga atttcgtaga gcagcgtttc ggcatcattg atgcggcgta
      901 tttcaaaaaa gacgacatca gcgaagtcat tgccgacaca gtgttcagca tggcgccggg
      961 cagcgtttat ggcccgtatt tggacaacgg cgcttacatg gccgtcaaag tattggatcg
     1021 caaggtcatt cccgactccg ttcgttcccg ccacatcctc atccaggccg ccaatccgat
     1081 ggaactggca acggctcagg ctcgtgtgga cagtctcaaa ggcgtcatcg aatccggcgc
     1141 ggctaccttt gaagcgttgg cggctcaatt cggcagcgac gcgaccaaag ataaaggcgg
     1201 cgacctagat tttgctgcgc ccggcggcat ggtgaagccc ttcaacgacc ttatcttctt
     1261 tgaagcagag caaggcaaac tctacagcgt agtgacgcag ttcggcgtac acttagtgga
     1321 ggtgactgcc aaaaaattca tcaacaacaa taccggcgtg aaactggcct acctcatgga
     1381 gcgcatcatg cccagtgaca aaacacaggg caatgtaaaa gacaaggcca ttgcgctgat
     1441 gagcggcaac cggaaattgg aagacttggt ggcagccgcc aaaaaagaca agagtctgag
     1501 cctcgccgtt acgcctttgc tggaagccaa cgattacaat attgccgatt tgggcagtgg
     1561 tcaatcgggc cgcgacatgg tgcgctgggc cttcaccgcc aaaaaaggcg aggtatctcc
     1621 ggaggtgttt tcattccaaa acgctgaatt gctgacggtt gataaatttg tgttggccgg
     1681 tttgaaaacc attcagcccg ccggcttgcc ctctgtggaa aacatccgcg aggagatcaa
     1741 acagttggta ctcaacaaaa agaaaggcga agtattggca gatcgcatga aaggcatgga
     1801 cttgcaggca ttggcatcca aattcaagac cgagatagac acggcccaat ccatcagttt
     1861 cggttccgga tttttgcccg atctgggcgc agagccgaaa gtaatagcta cggctgccgc
     1921 tctggcgctc aacaaaacct ccaaacccat tgtcggttct tccggggtat ttgtggtgca
     1981 ggtttacaac aagcccgaaa tggctccggc agcggattat accgctacca agacctcctt
     2041 atcctcgccc agccgcgctc aaattccttc catgattatg caggcactga tgaaaaatgc
     2101 aaacatcaag gacagcaggt ctaagttttt ctaactataa cttatccatc atcagggctt
     2161 tttgtctctg atcggattct ttcctcaaca ttcgatcccg tgcttcgggg tcgaatgttt
     2221 tttttaatga caatttgttg cgaatgaaat acaccgccat cggcctcatg tccggctcct
     2281 ccttggatgg tttggatata gccgcctgca cctttgaaat ggatggagac aaagtagtcg
     2341 gctgggcttt gctgcaaggc gaaacacagc ccttcccgga ggcctggcaa gagcggctga
     2401 ggcggctgcc ggaatccgat gcacgttctc tggcgtcggc acacagtgaa ttgggccgct
     2461 ggatgggaca atgcgtggcc gacttcatgc aacggcacgc ccttcgcccc gatctggtag
     2521 cctcgcatgg gcacactatt tttcattttc cggcagagcg attcactaca caaataggcg
     2581 atggggcagc tattgccgcg gcctgcggat gtccggtagt ctgcgatttc agaaccggcg
     2641 atatggcgct cggcgggcag ggcgcaccgc tggcgccgct tgccgacagg atgctgtttc
     2701 ccgactacga cgtgctgctc aatgtcggtg gcatagccaa tatcacctgc aaactcggca
     2761 gccgctatgt ggcctgcgat gtgggcggcg ccaatcagat actcaacgca ttggctcaaa
     2821 cgctgggact gccttacgac gacggcggac ggctggccgc ttcggggcag tacattccgg
     2881 ccttggcgga agctatggat gcgctgccgt acttttcctg gccgccgccc aaatcgctgg
     2941 gcaacgactg ggtgcaagcc catcagacac aggcgtatct gaactggccg gcatcggttg
     3001 aagaccgcct gcataccgct tgccggcaat tggcccgaca aacagcgggg gccatacagc
     3061 gcatttttga atcagaaaaa cagagccgtc ctgcttatcg gatgttggct accggcggcg
     3121 gcgcttacaa cactttcctc gccaactgca ttgtagaaga atgcacctcg ctgatgggcg
     3181 aatgtgtttt ggaactgccg gatgcaggca tcgtcaatta taaagaggct gtgttgatgg
     3241 cgctcatggg cgtgctgcgc gtattggaaa ttcccaactg catagcgagc gcaaccggcg
     3301 cggagcggga tgctgtcggg ggagccgttt atttgggata gataacatgg aaaaatggac
     3361 aaaaataaac tcatcttcgt tacgggcgga accggctttc tggggtccta tttgcttcgg
     3421 tatttggtaa aagaaggcta tacccatatc cgtgctctga ggcgcagcgg cagctctatg
     3481 gatttggtac aggacgtagc cggtcgcatc gaatggatag agggtgatgt gctcaatcca
     3541 ttttcgctgg aagagggcat gtcgggcgcc gcacaggtat atcattgtgc tgcagcggtg
     3601 tcttatcagg ccgccgacaa agcgcgcatc atgtcggtca atgtggaggg cactgccaac
     3661 gtggtcaacg aagcattgga ggcgggcatc gaaaaaatgg tttttgccgg ctcggtggcg
     3721 gccttggggc aacccaaaga aggcttcaaa accaccgaaa ctacgccctg ggagtggaaa
     3781 aaagaattgt cgagctacgc ggtgagcaag cggctggccg agctggaagt gtggcggggc
     3841 gctgctgaag ggctgaacgt gtgcgtgatc aatccttccg ttattctggg cgccggccgc
     3901 tgggacgacg ggccgctcaa gattttctct ttggtccatc gcaattttcc gatttgccca
     3961 cccggtcgca gcggctttgt ggacgtgcgg gacgtggcgc agtccatgat gcgcatcatg
     4021 gagagcgaca tttcgggcga gcgcttcatc gtgtcggcag aaaacatgta ttttcaggaa
     4081 ttgatggccc tgatggctca agccttagac aaaaaagccc cgaccatcaa agcaggcaac
     4141 gcgctgatgc agttggccag aataggcgat gctgttggcg ccctgctgac cggccgtccg
     4201 gcagagatca cccgcgcagc catttaccac tcgtcgttgc tgtcggagta cgacaacagc
     4261 aaactcgtgc aggcgcttgg ctacagctat aggcccattt cggagaccat tcgggaggca
     4321 ggggctttgt ttnnnnnnnn nnagcgatgc gcagcgacaa aggatcgcgg gtattgccct
     4381 tgaaataaac tacaaatcag gcctcttctt caaacggatc gctgtccaaa ttgatgggcc
     4441 gatccacgcc ttcttctaat gaaataaagg tttctgtgcg ctgtatgccc ggtattttct
     4501 ggattttatc gtgcaatact tcgcgcaggt gattggtgtc gcggcagagg atttttgcaa
     4561 aaatgttgta cagaccggtg gtataatgca ggctcaccac ctcggggatt tcctgaagtc
     4621 gctcggcagc atcatcgtat aaagaactct tatctaaata aataccgagg aaagcgcaga
     4681 tgtcgtaacc cagtttggag tgatctactg aaagattgta acccttgnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatttgctc atcaaatcgt caattttgag
     4921 caacgaaagt atcaaaaaaa attacaattg ctaatgaatc ggtagatttt ttgagatatt
     4981 ggtaaatatt gtacaaataa ccctgagtaa cagacaagcg cgtttactta tttttttcct
     5041 tctcatttcg ttatctttgc aaaaaatcga agccatgctg aagagattac cgtcgggaga
     5101 atcgagtttt gaaaaaatac aaacgggcaa gcaactgtat gtggacaaga cccgccagat
     5161 atacgaattg ctcagttccg gcgatttttt cttcctctcc cgcccgcgtc gtttcggcaa
     5221 gtccttgacg gtatccacac tcaaagcgct ttttgaaggg cgccgggagt tgttcgaagg
     5281 gctgtggatt gccgagcacg gcggctggga ctggagcaaa acaaatcccg tcatctctct
     5341 gtctttcaat gggttggatt acaaaacgca tggattggag aaggcgctga cgctggaatt
     5401 ggataaaata gctgccgcca acggattgcc tcagtctggt ctgtctccca aagataaatt
     5461 gcgggaattg attgtaggtt tgagccaacg aggcccggta gttattttga tagacgaata
     5521 cgacaagccc atcaacgact acctgaccga ttacgaaatg gccgatacca accgcgatgt
     5581 gctggggaat ttttacggcg cgctcaaggc caacgacatc accgacaatc tccgctttgt
     5641 gttcatcacg g
//
LOCUS       DAORLF010000317         5643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7811_length_5643_cov_2.676628, whole genome shotgun sequence.
ACCESSION   DAORLF010000317 DAORLF010000000
VERSION     DAORLF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7811_length_5643_cov_2.676628"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..716)
                     /locus_tag="PK198_20035"
     CDS             complement(180..716)
                     /locus_tag="PK198_20035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF41097.1"
                     /translation="MAPDFLFVSDQLHLSAFRESDKSDLIHWLQDEIVHQYTLHMPFP
                     YGEKEADEWLELTRKMEEKHACRPNWAIRNAEGRLIGGIGRSMHKGEENHWDEIGYWL
                     AAPYRGKGVMTSVVDFFCRYWFEHSPLVRIEAAVFDINPASARVLEKTGFEYEGRLRK
                     KYFKNGRFLDGLMYGRLK"
     gene            788..1417
                     /locus_tag="PK198_20040"
     CDS             788..1417
                     /locus_tag="PK198_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF41098.1"
                     /translation="MFSNLQISHTTDKAVQGSSDRTVSAEAMHAEWLEVQAAQRDPVM
                     FRSLYERYYEPVFRYLYNRSQDEDLAADLCAQVFLKALQRLGDYTFQGVPFSAWLYRI
                     AANEIAGHYRQAQKTRIVSVEEGGLHALVDEIEEDHSEARRTLLLTAMQSLKEADLAM
                     IEMRFFEQRSFKEIADILGITESNAKVKTYRILERMKGVMINNQTASGL"
     assembly_gap    1577..1620
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1621..>1979
                     /locus_tag="PK198_20045"
     CDS             <1621..>1979
                     /locus_tag="PK198_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41099.1"
                     /translation="VALLLIFRTPGTTSDARRQTAAWEKQLITPPPLPQIELPYQTHI
                     LPSAQVTSLEGNPHITVPADAFIDQSGRPVSGDVELRFRKLDDFVDFFLAGIKLTQDS
                     GNVKRQSIFVVVPMWAP"
     assembly_gap    1980..1989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1990..3161
                     /locus_tag="PK198_20050"
     CDS             <1990..3161
                     /locus_tag="PK198_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41100.1"
                     /translation="VEIYAEQNGQRVFLAPGKIVQLSFESQIELVSNGKMPAFYEYFL
                     DADKNAWMNQGLSRTEVIGQGDLNPSDPLYEEKQALYDALVRMDDRAAKSKEQWLAGN
                     PAPTVPAKPQKSNPDQPTIELDFLANAVPQTADGQKAKLYQGAIWQISPRSPAYDQRA
                     FGVTWQSAKLTQIADSEDYELLLSHGATSLQLIVNPVLTGSAYDQALSQWRSAYADYE
                     RALADWKKRSTEALAEIDRQAEKDKTTAIKTREKEWADAGTTGAKLLMKLRHEMTIDR
                     LGLWAIAQPLPTTPTIAAGRITDQFGNAYEQRVAYVVHPGHNTLHQVMISGKSRIPIA
                     PGSTLWAVHPNGKIAVLRPSDLQQAASNVTSNKLVLDLKEPAPASKEELRKILQL"
     gene            3302..>3863
                     /locus_tag="PK198_20055"
     CDS             3302..>3863
                     /locus_tag="PK198_20055"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF41101.1"
                     /translation="MTVIHTNTLLKVLACVAFSCCLISNTWSQAVVLPEGGVNHKSWA
                     GRRINVTDVEIRWNAPAVRGREGQIWGTPIAHYGYSILGYGSNVESPWRAGANESTTI
                     SFSTDVLIEGKPLATGQYGFFIALYPDSCVLIFSKNTTGWGSYFYQADADVLRVVVRQ
                     QKDIPQSRERLEYTFSDQTDRSVVVAL"
     assembly_gap    3864..3878
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3879..4452
                     /locus_tag="PK198_20060"
     CDS             <3879..4452
                     /locus_tag="PK198_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41102.1"
                     /translation="WERWRIPFKVEVDHIQTGLASIQRQMRGALGFDPPSLQAAAQWC
                     LINDVNTEEALVWIHTATDPNLGGLATFAVLSTKAGLLRKVGRIQEADAAMEKALANA
                     TLLEMHGYGRQLIGQKKYQEAFAVFEKNFKKHGDAWPTHVGMMRAYSAVGDLKNALKH
                     AKIALSQAPDDLNKRSLEGMIKTLEAGKSL"
     gene            complement(4826..>5643)
                     /locus_tag="PK198_20065"
     CDS             complement(4826..>5643)
                     /locus_tag="PK198_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRF41103.1"
                     /translation="GKPVVGFPTPSRKNEFYSQTMADWKWPEYTIPTYIKSHIHEEQM
                     DRSKNEYPLVPTFRLPTLIHSRSGNAKWLYEISNRNPIWMHTSDAERLGVQTGDLLRV
                     NTEIGYFVDKVWVTEGMKPGVVACSHHLGRWRRPQDVPGNRWATNTVAIENDGKGGWR
                     MRTMDHIKPFKSSDPDSARIFWSDGGVHQNITHAVHPDPISGMHCWHQKVRLEKAHPE
                     DTYGDIFVDTNKSFEVYKKWQAMTRPAPGPDGLRRPLWMKRALRPAEETFYIK"
BASE COUNT         1380 a         1544 c         1421 g         1229 t
ORIGIN      
        1 gaaataaagc gggttcgagg ttttgggttt gagatttcgg ggtcaaggtt tcgggggtag
       61 tgaaggggtg actcggcaag tttcagcccc gttagtcacc ccttcactaa gcagggcact
      121 cacccctcac ttctcacaca ctcacccctc acccctgaaa accgcctacc gaaaaccgcc
      181 tacttcaacc tcccgtacat cagcccgtcc aaaaaacgtc cgtttttgaa atactttttg
      241 cgcaagcgcc cttcgtactc aaaaccagtt ttttccagca ccctggccga ggcaggattg
      301 atgtcgaaca cagccgcctc tatgcgcacc aaaggcgaat gttcaaacca gtaccggcaa
      361 aagaaatcta ccaccgaagt cattacccct ttgccgcgat aaggcgccgc cagccaatag
      421 ccgatttcat cccaatgatt ttcttctcct ttgtgcatac ttctcccaat accgccgatg
      481 agccgacctt ctgcattacg gatggcccag ttgggacggc aggcatgctt ctcctccatt
      541 tttcgggtaa gttccagcca ctcgtcagcc tctttttccc cgtaagggaa aggcatgtgc
      601 agcgtatatt gatgaacaat ttcgtcctga agccagtgta tcaggtcgct tttatcgctt
      661 tcgcgaaagg cagaaaggtg taattgatca gaaacgaata aaaagtcggg agccatgatg
      721 tatctttttt gcaaatttgc attatgaaag tgaaacagcc gagaaacaag gaaaaattcc
      781 ggagctgatg ttttcgaacc tacaaattag ccatactaca gataaggcgg ttcagggcag
      841 cagcgaccga actgtttccg ctgaggccat gcacgccgaa tggttggaag tgcaggcggc
      901 tcagcgcgat cctgtcatgt ttcgttcctt gtacgagcgt tactacgagc ctgtgttcag
      961 gtatttgtac aaccgctctc aagacgaaga cttggccgct gatttgtgcg ctcaggtgtt
     1021 tctcaaagcc ctgcaaaggc tgggcgatta cacgtttcag ggagtgcctt tttcggcctg
     1081 gctctatcga atagcagcca acgaaatagc agggcactac cgacaggcac aaaaaacgcg
     1141 catcgtcagc gtagaggaag gcgggctgca tgccttggtg gacgagattg aggaagacca
     1201 ctcggaggcc cggcgcaccc ttttgctcac cgccatgcag tcgctcaaag aagcggactt
     1261 agccatgatc gagatgcgct tcttcgaaca acggtctttt aaagaaatag ctgatattct
     1321 gggcattaca gagagcaatg ccaaagtgaa aacataccgg attttggaac gaatgaaagg
     1381 ggtaatgatt aataaccaaa cggcttccgg gctttgaagc tgccataaga aatgcgtatg
     1441 aagaacgagt acgactttcg caccactccc cgccctttgt cgagcgaaga catcgcccga
     1501 cataaggatt ttgacgctct gctgcgccaa caccgctccg gcaagcgtcg caccggcatt
     1561 gtccagatgc gccgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 ggtggcttta ttgctcattt tcagaacgcc cggaaccacc tcagacgccc gccgacaaac
     1681 cgctgcatgg gaaaagcagc tcatcacgcc gccgccactg ccccagattg aacttccgta
     1741 ccaaacacac atactgccct ccgcacaagt tacaagcctc gaaggcaacc cgcacatcac
     1801 cgtccccgca gatgctttta ttgaccaaag cggtcggccc gtttccggcg atgtggaatt
     1861 gcgtttccgc aaattggatg atttcgttga tttctttctc gcaggcatca aactgacaca
     1921 ggattcgggc aatgtaaaac gccagtcaat atttgtagtc gttcctatgt gggctcccan
     1981 nnnnnnnnnt ggtagaaatt tatgccgaac aaaacggcca acgcgtattt ctggcgcccg
     2041 gcaaaatcgt tcagctttct tttgaatccc aaatcgaact cgtttccaac ggcaaaatgc
     2101 ccgcctttta cgaatacttt ctggatgccg acaaaaatgc ctggatgaat cagggactca
     2161 gtcgcacgga agtcatcggg cagggcgacc tgaacccctc cgacccgctt tacgaagaaa
     2221 agcaagcact ctacgatgcg ctggtccgca tggatgaccg tgcagcaaag tcgaaagaac
     2281 aatggttggc cggcaatccg gcgcccacag tgcccgccaa accccaaaag agcaacccgg
     2341 accagcccac catcgagctg gacttcttag ccaatgccgt gccgcaaacc gccgacgggc
     2401 aaaaagcaaa attgtatcag ggcgccatct ggcaaatttc gccgcgcagc cccgcctacg
     2461 atcagcgcgc tttcggcgtc acctggcaat cggccaaact cacgcaaatc gcagattcag
     2521 aggattatga attgctgctt agccacggcg ctacctcgct ccaactcatc gtcaatccgg
     2581 tgctcaccgg cagcgcttac gaccaggccc tgagccaatg gcgcagcgct tatgccgact
     2641 acgagcgcgc attggccgac tggaaaaagc gctctacgga ggcattggca gaaatagacc
     2701 gccaggcaga aaaagacaaa actacagcca ttaaaacccg ggaaaaagaa tgggccgacg
     2761 caggcacaac gggcgccaag ctcctgatga aactgcgcca cgaaatgacc attgaccgct
     2821 tggggctgtg ggcgattgca caaccgctgc cgactactcc taccatcgca gcaggccgca
     2881 tcaccgacca attcggcaat gcctacgagc agcgggtagc ctatgtggta caccctggcc
     2941 ataacaccct ccatcaggtc atgatttccg gcaaaagccg catccccatt gcgccgggca
     3001 gcacgctttg ggccgttcac cccaacggaa aaatagccgt tttgcgcccc agcgacctcc
     3061 aacaggccgc ttctaatgtg acttccaaca aattggtatt ggacctcaag gagcctgctc
     3121 ctgcctcgaa ggaagaactt agaaagatat tgcagctttg aggtgatgtt gtgttgaacg
     3181 agagatgttt cagtacttcc aaacacaaga caaagtagta ctcaaatggt ggctgacagg
     3241 tgatgacaaa ttttcatttc acctctctac atagtattat ttcaccaaaa tcaccaacaa
     3301 aatgactgta atacacacca acacactgct caaagtgctg gcatgcgtcg cattttcatg
     3361 ctgtctgatt tccaatacat ggagccaggc ggttgtattg cccgaaggcg gcgtcaacca
     3421 caaaagctgg gccgggcgcc gcatcaacgt tacagatgtg gaaatccgct ggaatgcccc
     3481 tgccgtgaga ggccgggaag gccaaatatg gggcacgccc attgcccact acggttattc
     3541 gattttgggg tacggttcta atgttgagtc gccctggcgc gcaggcgcca atgaaagcac
     3601 gaccatttcc ttctccacag atgtgcttat tgaaggtaag cccctggcca ccggtcaata
     3661 tggcttcttc atcgcccttt acccggattc ctgtgtcctg attttttcta aaaacacgac
     3721 aggctggggc agctattttt atcaggcaga tgcagacgtg ttgcgagtag ttgtgcgcca
     3781 acaaaaggac atcccccaaa gccgggagcg attggaatat acattttcag accaaaccga
     3841 ccgttcggta gttgtagctt tggnnnnnnn nnnnnnnnat gggagcgttg gcgcattccc
     3901 ttcaaggtgg aggttgacca catacaaacg gggctggctt ctatacaacg ccagatgcgc
     3961 ggcgctttgg gcttcgaccc gccgagcttg caggcggcgg cgcagtggtg cctcatcaac
     4021 gacgtcaaca cagaggaagc gctcgtttgg attcatacgg ccaccgatcc taatttgggc
     4081 ggtctggcca cttttgcagt tctttccacc aaagcgggat tgctgcgcaa agtggggcgc
     4141 attcaggaag cagacgcagc gatggaaaaa gccctggcta acgctacgct gcttgaaatg
     4201 cacggctatg ggcggcaact gatcggccag aaaaaatatc aggaggcctt tgcggttttt
     4261 gagaaaaact tcaaaaagca tggagatgcc tggcctaccc atgtgggcat gatgcgcgcc
     4321 tattctgctg tcggcgatct gaaaaacgcg ctgaagcacg ccaaaatcgc actctctcag
     4381 gcacccgacg acctcaataa aagatcgctg gaaggcatga ttaaaacttt ggaggcgggg
     4441 aaatcgctgt agtaaaacga aaatagattt acattttctt aaggccaaag aattagataa
     4501 gacgcttaaa atgaagcttt tacttatcta atcctttggc taaaatgtaa atatgttttt
     4561 ttataaatac aggaccctga gtcggtaggc agtaggtgcg ccatcgcgct gaaaccggag
     4621 gttatgcaca aaacctggcg gcgcaaggct tagaaaaact actaaagcct gatggaggac
     4681 gactgacttg aaaaaaacta taaaaaaacc ggcatcccgg agccgttaca ccccgaaatg
     4741 ccggaatctt gaacgcggaa ccagtaggcg gcttgcagct tgcagccagc agcttgtagc
     4801 cgtttcaacc ttgcctcccc cgctcctact tgatataaaa cgtctcctct gcaggtcgca
     4861 aagcgcgctt catccacagc gggcggcgca gcccgtcagg accgggcgcc gggcgcgtca
     4921 tagcctgcca ctttttgtac acctcgaaag atttgttggt atccacaaaa atatcgccgt
     4981 aggtgtcctc cggatgcgcc ttttccaggc gtactttctg gtgccagcag tgcataccac
     5041 tgatggggtc gggatgcacc gcgtgcgtga tgttttggtg taccccgccg tcgctccaga
     5101 agatgcgggc gctgtctggg tcgctgctct tgaaaggctt gatgtgatcc atcgtgcgca
     5161 tgcgccagcc gcccttgccg tcgttttcga tggcgacggt attggtagcc cagcggttgc
     5221 ccggcacgtc ttgcggcctg cgccagcggc ccaggtgatg cgagcaggcc accacaccgg
     5281 gtttcatgcc ttcggtaacc cacaccttgt ccacaaaata tccgatctct gtgttcacgc
     5341 gcagcagatc gccggtttgt acgcccagcc gctctgcgtc ggaggtgtgc atccagatcg
     5401 ggttgcggtt ggagatttcg tacagccatt tggcgttgcc ggaacgcgag tgaatgagcg
     5461 tgggcaatcg gaaggtgggc accaatggat actcgttttt cgagcggtcc atctgctcct
     5521 cgtggatatg gctcttgatg taagtaggaa tggtgtattc cggccatttc cagtcggcca
     5581 tcgtttgcga gtagaactca tttttccgcg agggcgtggg gaaacctacc accggtttgc
     5641 cat
//
LOCUS       DAORLF010000318         5615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7876_length_5615_cov_1.807374, whole genome shotgun sequence.
ACCESSION   DAORLF010000318 DAORLF010000000
VERSION     DAORLF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_5615_cov_1.807374"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..824)
                     /gene="rlmD"
                     /locus_tag="PK198_20070"
     CDS             complement(216..824)
                     /gene="rlmD"
                     /locus_tag="PK198_20070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRF41104.1"
                     /translation="MFDLDMILYRGEPAIEEQLGHVRCRIGPKSFFQTNTRQAKVLFD
                     TAAEFAGLQGHENVYDLYTGIGSIALYLAGRCRQVTGIEEIAAAIDDARDNAALNGID
                     NCTFYAGDVKDMLTPEFAEKHGKPDVLITDPPRAGMHPKVVDILLELAAPRIVYVSCN
                     AATQARDLQLLSAKYRVLKVRPVDMFPHTHHVESVALLELQG"
     gene            complement(829..1617)
                     /locus_tag="PK198_20075"
     CDS             complement(829..1617)
                     /locus_tag="PK198_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41105.1"
                     /translation="MAKKTFIAEQVRITGIAEKGRGVGRDAEGRVFFVPDTVPGDVVN
                     VLTLRKKKGFFEGVVHAVLESSPERVQPFCRHFDLCGGCSWQHLDYAAQLHHKQLMVE
                     NALLRIGKLEVEDFQPITGADPNVYYRNKLEFSFSNRRWLTKEEVDSEVTNLEDVLGF
                     HKSGAFDKAIDIEHCWLQPDPSNAIRLAAKRIAVAQGLAFWDARDNKGFVRNLMLRVA
                     TTGQVMALMSFSENRKKAIPAFLDALQEACPDHLAVLLHQSQGQ"
     gene            complement(2062..2823)
                     /locus_tag="PK198_20080"
     CDS             complement(2062..2823)
                     /locus_tag="PK198_20080"
                     /inference="COORDINATES: protein motif:HMM:NF027605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4928 family protein"
                     /protein_id="HRF41106.1"
                     /translation="MNVDNQSNYTLAFEDGWDALQPQIVNDQDFLKAVLLMLDLMKQD
                     KNIDPSRYCDENGNLKSYVELCKYKKLQLVYNPEYPTWKIIHTLLSQAINEGKAGAVA
                     YHLVGATLQLRFPDIEISNESANTADQPTGRSGDFTIGDTVFHVTVHPMTSVFDKCRE
                     NIKDGLKAFLLVPDSSLTGSRMWALKDGVELFAVESLESFISQNIEEISAFDPKYRKN
                     SLFNLIYLYNQRVDQAETDKSLMIELPSNLKVSNG"
     gene            complement(2820..3560)
                     /locus_tag="PK198_20085"
     CDS             complement(2820..3560)
                     /locus_tag="PK198_20085"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HRF41107.1"
                     /translation="MHKTRNSAILIGLFLSRFDEDGLNALGFSGFWEAFNTLGYAIGV
                     KPSSIKNYRDEFDPYYPNTRKGWHKRSLRDYCKVVMEEYASYDLHSFTEIIKELLINN
                     YEVERIEEMIIDKKSTSSIAQRLITGQSAEAYFKENYLTVPQFQNYAFKDTTLLGCGF
                     DFLLTRQTDFFCIEVKGLHSKSGTISLTEKEYQMAEKLSEHFCLYVVSNFCEQPIANV
                     IINPLESGLDFVKDERLITVVSFQTYIR"
     assembly_gap    3673..3692
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3693..4240
                     /locus_tag="PK198_20090"
     CDS             <3693..4240
                     /locus_tag="PK198_20090"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF41108.1"
                     /translation="FEVQLVLNHMQAPESAVQFTSLLEDFDSALWGHHFVVPAAIGQV
                     FVEGNKRRVVCILNGSKPFQCALMPKGEGAFFINVNKKLRDQLRIKIGSPLEVTLWKD
                     DSEYGLPMPEELEELLRQDEEGHRCLHALTPGKLRTMLYYVGSPKTAETRLIRAVAVV
                     EHLKLMQGKIDFKILMGKKDF"
     gene            4403..>5235
                     /locus_tag="PK198_20095"
     CDS             4403..>5235
                     /locus_tag="PK198_20095"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF41109.1"
                     /translation="MRTLLLLLIPAALLFGCRPKNAGAEQAASTETMTDKGPVQFETA
                     VSDLRMRETPGEKGVIVATLPKGAKLEDLGEVSDFTTRVTLRGITFDEPWIKVKTSSG
                     QTGWVYGGGLIFDASQNSPATRALLQKRATAMLGQPLVESINQHRRNWAATRNEADLS
                     RLLEQSTALRDTINEVLQRRIPPTSAEDMPDMFWLAELFTGFQPQLVAEGTAYYLFLD
                     YRQWLALSKKTKGVQDDIFMDLQIAAFPDDSIEYFFPAWQIQTWDYGGHCMLGSGVCK
                     D"
     assembly_gap    5236..5300
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5301..5512
                     /locus_tag="PK198_20100"
     CDS             <5301..5512
                     /locus_tag="PK198_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41110.1"
                     /translation="TMKARIVSDLTQAETSFWHTAAEAGAELDAILNAKFKVLTQADV
                     VALSTLRDRLKDPAKHGIEAGKRNG"
BASE COUNT         1380 a         1415 c         1309 g         1426 t
ORIGIN      
        1 tgactttttt cgcgggcgcc ctgccccata ttggcggcct ggtctttgat aacgccgccg
       61 gcatcgtaaa gcgcctgcca aagtttttct atcatatatt ttttatgttt tcgagttcaa
      121 agtttcgggt ttcatccgcc gaagccttgg cgaaggcgga ttcgggttgc tcaccactca
      181 catactcacc cttcacccac tcacccctca cccactcacc cctgaagctc cagcagtgcc
      241 acgctctcca catggtgcgt atgcggaaac atgtccaccg ggcgaacttt gagcacgcgg
      301 tattttgccg agagcaattg cagatcgcgg gcctgggtgg cggcattgca actcacatac
      361 acgatgcgcg gcgcggccaa ttccagcagg atgtccacca ctttggggtg catgcccgcc
      421 cgcggcggat cggtgatgag cacatcgggt ttgccatgct tttcagcaaa ctccggcgtg
      481 agcatgtcct tcacgtctcc ggcgtaaaaa gtgcaattat caatgccgtt gagggccgcg
      541 ttgtcgcgcg catcgtcaat ggcggcggct atttcctcaa tgcccgtcac ctgtcggcaa
      601 cgccccgcca gatacaacgc tatactgccg attccggtat agagatcata cacgttttcg
      661 tggccttgca gcccggcaaa ctcggcagcc gtatcgaaca gtaccttagc ctgcctggtg
      721 ttggtctgga aaaaagattt cggcccgatg cggcaccgta catggcccaa ctgctcctcg
      781 atagcaggct cgccgcgata caggatcatg tcgaggtcga acataaaatc attgaccttg
      841 tgattgatgc aatagtacag cgaggtgatc tgggcaggcc tcctgcaatg catccaaaaa
      901 agcggggatg gcttttttgc gattttcgga aaaactcatc agcgccatga cctgcccagt
      961 agtggccacg cgcagcatga gattgcgcac aaaacccttg ttgtcgcggg catcccagaa
     1021 ggcaagaccc tgcgctacag cgatacgttt ggcggccaga cggatggcgt tggaaggatc
     1081 gggttgcagc cagcagtgtt caatatcaat ggctttgtcg aaggctcccg atttgtgaaa
     1141 tcccagtaca tcctccaggt tggtcacctc agagtcaact tcctctttgg tgagccagcg
     1201 gcggttggag aaggaaaact ctaatttatt tcggtaatat acattgggat cagcgcctgt
     1261 gatgggctga aaatcttcta cttccaattt accgatgcgc aacagggcgt tttctaccat
     1321 cagttgtttg tggtgcaatt gggcggcata gtctaagtgc tgccaactgc atccgccgca
     1381 gaggtcgaaa tgccggcaaa agggctgcac acgctccggc gagctttcca gcacggcatg
     1441 caccacgcct tcaaaaaatc cctttttctt gcgcagggtc agcacattca ctacatcgcc
     1501 gggcacggta tcgggtacaa aaaatacgcg tccctcagcg tctcggccta cgccccgccc
     1561 tttttctgct atgccggtga tgcgcacctg ctccgctatg aaagtttttt ttgccataag
     1621 gatggcaaag gtacgatgtt ttgggggaga tcaaatcaat tctaatccga ttgtaacgcg
     1681 ctgagcaaac gagattcgta gctacctttt ttcaattcac attcccatat acgaattaca
     1741 gtccatcctt ttttttgtaa agcattggtt accaagatat ctctggcaat gttcctgtct
     1801 cgcttggatt cccaatatgt gcgattctga gcgggtttag tatttcggca atcgtggcca
     1861 tgccaaaagc aaccatctgc gaatatgaca attctttgtt taggaaagac aaaatcaggc
     1921 ttcccaacga gaggataact tctcctccat cctttaattt tatgattttt gaaaaactgt
     1981 atcagtttca gttccgtgga tgcgttgcgg gaagacttta ctcttcgcat aacctcgcta
     2041 cgtttttcgg gagtgaaaac atcagccatt tgacaccttc aaattggagg gtaattcgat
     2101 catgagcgac ttatctgttt cagcttggtc aaccctttga ttgtacaaat agatcaagtt
     2161 gaaaaggctg tttttccggt atttaggatc aaatgcacta atttcctcaa tgttttgaga
     2221 aatgaacgac tctaaagatt ctaccgcaaa aagctccacg ccatctttta atgcccacat
     2281 ccgagaaccc gtcaaactgg aatctggaac cagcaaaaat gctttaagcc catccttgat
     2341 attctccctg cacttatcaa aaacagaggt cattggatga accgtgacat gaaaaacagt
     2401 gtcgccaata gtgaaatcgc ccgatcttcc tgttggctga tctgcggtat tcgctgattc
     2461 atttgagatt tcaatgtccg ggaatctcaa ttgcaaagtt gctccaacca aatgatatgc
     2521 aacggcccct gctttacctt catttattgc ttgggagagt agcgtatgaa tgatcttcca
     2581 tgttggatat tcaggattat agaccaactg aagtttttta tacttacaca gttcgacgta
     2641 acttttcaaa ttgccgtttt catcacaata tctgctgggg tcaatatttt tatcttgttt
     2701 catcaaatca agcatcaata aaacagcttt taaaaaatcc tgatcattga cgatttgagg
     2761 ctgaagcgca tcccagccat cctcaaaggc caatgtatag ttggactgat tatctacatt
     2821 catcttatgt aggtttgaaa tgacacaaca gtaataagcc tctcatcttt tacaaaatcc
     2881 aacccagatt ctaagggatt gattattaca ttggcaatag gttgctcgca aaaattagac
     2941 acaacataca agcaaaaatg ctcgctcagc ttttcagcca tttgatactc cttctccgtc
     3001 agcgatatcg tacctgattt gctgtgaagc cctttcactt caatacaaaa aaaatcagtt
     3061 tgacgtgtaa ggaggaaatc gaagccacat cccagcaaag tagtgtcttt aaaggcgtaa
     3121 ttctggaact gtggcactgt caagtaattc tccttgaaat aggcttccgc ggattggcct
     3181 gttataagtc tttgcgcaat tgagcttgta cttttcttat caataatcat ttcctctatt
     3241 cgttcaactt catagttatt aatcaataac tccttgataa tctcagtaaa tgagtgtaaa
     3301 tcataactcg catattcctc catgacgacc ttgcaatagt ctctaaggct tcttttgtgc
     3361 cagccttttc ttgtatttgg ataatacgga tcaaactcgt ctctgtaatt ttttatagag
     3421 gaaggcttca ctcctatggc atatcctaac gtattgaatg cttcccaaaa tccgctaaag
     3481 ccaagtgcgt taaggccatc ttcatcaaac cttgataaaa ataatcctat caggatcgca
     3541 ctattgcggg tcttatgcat agcagataat tagatatgcg tttgagaaaa aaattccgca
     3601 accgtctttg ggtatgtttt cataaaacaa attgagtttt tttcattatt aggaacaaag
     3661 ataccacaaa gannnnnnnn nnnnnnnnnn nnactttgaa gttcaacttg ttctcaacca
     3721 catgcaagca cccgaatctg ccgttcaatt cacttccctg ctcgaggact tcgactcggc
     3781 gttgtggggg catcactttg tagtgccggc cgccatcggg caggtctttg tggagggcaa
     3841 caagcgccgc gtggtgtgca tcctcaacgg cagcaagcct tttcagtgcg cgctgatgcc
     3901 caaaggcgag ggggcgtttt ttatcaatgt gaacaaaaaa ctgcgcgatc agctccgcat
     3961 caaaatcggc tcaccgttag aggtcaccct gtggaaagac gacagcgaat acggcctgcc
     4021 catgcccgaa gagttggagg aattgctgcg acaagacgag gagggccatc gctgtctgca
     4081 cgccctcacg cccggcaagt tgcgcactat gctctattac gtcggctcgc cgaaaactgc
     4141 cgaaacccgc cttatccggg ccgtagccgt agtggagcac ctgaagctga tgcagggcaa
     4201 aattgatttc aaaatactga tgggcaaaaa ggatttctaa tgtgtagatg tgctgattgc
     4261 tgatgcttgc cgcgcgtgtg gcgcggtgct gattactaac ttagagtgtt caggaaagta
     4321 tcgaatactt ttttgacttc ttactattcc gctttgcctg tcgcccggaa cattccaacc
     4381 aatttcaata aacaatcaca tcatgcgtac cttacttcta ttgctgatac ctgccgccct
     4441 gctgttcggc tgtcgcccca aaaatgccgg cgcagaacag gccgcctcta ccgaaaccat
     4501 gaccgacaaa gggccggtgc agtttgaaac ggccgtgtcc gacctccgca tgcgcgaaac
     4561 gcccggcgaa aaaggcgtca tcgtggccac cctgcccaaa ggcgccaaac tggaagacct
     4621 gggcgaagtg agcgatttca ccacgcgggt cactttgcgc ggtatcactt ttgacgaacc
     4681 ctggatcaag gtgaaaacct cctccggcca aacgggctgg gtgtatggcg gaggactcat
     4741 ttttgacgcc tcccaaaact cgcccgccac ccgcgccctc ctgcaaaaac gcgccacagc
     4801 catgctcgga cagccgctcg tggagagcat caaccaacac cgccgcaact gggccgctac
     4861 ccgcaacgaa gccgacctga gccgcctgct ggaacaaagt acggccctgc gcgacaccat
     4921 caacgaggtg ctgcaacggc gcattccgcc tacttctgcc gaggatatgc ccgacatgtt
     4981 ctggctggcg gagctgttca cgggcttcca gccgcaattg gtggccgagg ggaccgcgta
     5041 ctacctcttt ttggattatc ggcagtggct ggctctttcc aaaaaaacca aaggcgtaca
     5101 ggatgacatt tttatggacc tccaaattgc cgccttcccc gacgacagca tcgagtattt
     5161 cttcccggcc tggcagattc agacttggga ttacggcggg cattgtatgc tcggcagcgg
     5221 cgtttgtaaa gatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn aaaccatgaa agcccgcatc gtatctgatc tgactcaggc
     5341 cgaaacgagc ttctggcata cagcagccga ggccggcgcg gaactggacg ctatcctgaa
     5401 tgcaaaattc aaggtactga ctcaagccga tgtggtggcg ctttctaccc ttcgggacag
     5461 gctgaaagac ccggccaagc atggtattga agcgggtaag aggaatgggt gagtgggtga
     5521 gtgggtgagt gggtagtggg tgaatgggtg aatgggtgag tgggtgaggg atgaagggtg
     5581 agtggaaaaa gtatttgcta aagtaatgct accga
//
LOCUS       DAORLF010000319         5591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7905_length_5591_cov_1.637464, whole genome shotgun sequence.
ACCESSION   DAORLF010000319 DAORLF010000000
VERSION     DAORLF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_5591_cov_1.637464"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>332
                     /locus_tag="PK198_20105"
     CDS             <1..>332
                     /locus_tag="PK198_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41111.1"
                     /translation="TFPNYTLPVGGYVIVAIDSAKFRMAFNVPAFQWNTGQALNNTGE
                     LITLVNNTDAIMDTVRYSATAMPWPASANGGGPSVQLCNPASDNSNGANWAASVAPTG
                     FFINGIEV"
     assembly_gap    333..342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <343..>923
                     /locus_tag="PK198_20110"
     CDS             <343..>923
                     /locus_tag="PK198_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease I"
                     /protein_id="HRF41112.1"
                     /translation="ATPGAANNCAPPMPVSYPPYEIGVVTTNNANGVTDSLNVRCQLQ
                     GTVYGINLRTTGLQFTMIDDQNDGIAVFSTTADFGYQVEESNEIIIRGRITQFNGLTQ
                     ILPDTVIVVAQSNPLIPATTVTQLDESTESQLVRIENLTLVDPAQWTNAGTGFNVDAT
                     NGTNTFQIRIVSTSNIFGTPAPAGTFNLTGIGG"
     assembly_gap    924..942
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <943..1283
                     /locus_tag="PK198_20115"
     CDS             <943..1283
                     /locus_tag="PK198_20115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41113.1"
                     /translation="QFDSSSPYTEGYQIFPRYLSDIDIINAVSNPVKVNQGVRVSPNP
                     ALGVVLIKSESAFDAVRVTDAAGRTVYYNWFAPVTELNLDTTTWKRGTYHVAVMSGKA
                     TRLVQVVKAK"
     gene            1462..2493
                     /locus_tag="PK198_20120"
     CDS             1462..2493
                     /locus_tag="PK198_20120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF41114.1"
                     /translation="MKHFRLLAFCLSALWINAIDAQDIHYTLFNMSPLTLNPALTGAY
                     EGTARIGGIYRDQWSNVIAKGFKTPGVYLDAPIIRGFGKKDWVGVGAINVTDEAGRFD
                     LETTISGLSLAYHAAVGDKSVFTIGLQGARVQRKVNGASPYTRFEDAYDVNTQSFTGI
                     SADRGAIRPNINYTDINAGLLFRTKLADKQSLEIGVSAAHINAPRYSMSDSTGRNDPG
                     TRRPLRLAAHAQYITPLNEKWDLMPTVLFQSTAGAFEGVAQAWAGYNIKKDVRINLGA
                     GYRLGDAFQVLIGMDYEDLKVAASYDMNVSSLAPVSKTVGGFEVSAWYILKMYKKPDV
                     KPVIFCPRF"
     assembly_gap    2526..2576
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2577..>3062
                     /locus_tag="PK198_20125"
     CDS             <2577..>3062
                     /locus_tag="PK198_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41115.1"
                     /translation="RLFLFIAFCSIVSAQQLWAQPIRKSNYDKIVELAYEKLAEKDYY
                     NAITKFEEAYEEREDKTFLPILADLNYLVRDFVKAERYYARIFRADKTNEFAAKRYFY
                     ARSLKYNGKYEEAIPEFQKVLETATKDSLRTLIRNEISGAEMAMELGIANSDGTIGKG
                     V"
     assembly_gap    3063..3199
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3200..>3554
                     /locus_tag="PK198_20130"
     CDS             <3200..>3554
                     /locus_tag="PK198_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41116.1"
                     /translation="FAQVFKSGKGDRGWKKPEPLGEKINRPDAQIINVAFSPKDNRVY
                     ITQGVLEGNVISDAKIYVAAGTDGAANEVKGINGNYIAKHPAVGELFGREVLYFSSNM
                     PGGFGGFDLYYATRTG"
     assembly_gap    3555..3607
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3608..>3980
                     /locus_tag="PK198_20135"
     CDS             <3608..>3980
                     /locus_tag="PK198_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41117.1"
                     /translation="NTPGNEETPFFRDGTLYFSSDGHPTFGGYDLFFTVWDGTSWSAP
                     ANMGAGYNTSVDDQSLSLDAEGYKGFLTSNRAGGRSLRSKTCCDDIYAFEIARISANL
                     VVGVFDDAKKPLNGTSIGLIEM"
     assembly_gap    3981..3990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3991..>4356
                     /locus_tag="PK198_20140"
     CDS             <3991..>4356
                     /locus_tag="PK198_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41118.1"
                     /translation="NEKPGKIDSRTNANGNRYEFPLALDMPYKIVVSKEGYHPDSTTI
                     TTANLKENKTFEHRFYLKAKPVPPPVPEYDTITQEKPIVLENILYDFDKDVIKPESEP
                     DLQVVFELMTEYPDMVIEL"
     assembly_gap    4357..4458
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4459..>4831
                     /locus_tag="PK198_20145"
     CDS             <4459..>4831
                     /locus_tag="PK198_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41119.1"
                     /translation="RERINAVGYGETVPQTVSAKAAAQSNFLKEGDVLTPEYIDKLAS
                     EEQKEAAHALNRRTEFKIVKGPTSIIIKSTRLRKVEPTTKTAPNKQSQPAAAPKPAQS
                     SAPVMEFKEKFVDFGSVKKGE"
     assembly_gap    4832..4938
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5304..5328
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5329..5563)
                     /locus_tag="PK198_20150"
     CDS             complement(<5329..5563)
                     /locus_tag="PK198_20150"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRF41120.1"
                     /translation="MDIFHIDLQEVIINNLTDGIFFARMVCQRDGELYEIDARTSDAL
                     ALAARFHCPIHTYEFIMETAGIILEEEEPQPRAR"
BASE COUNT         1275 a         1330 c         1285 g         1187 t
ORIGIN      
        1 tacatttccc aactacacgc tcccggtcgg cggctatgtc atagtagcca ttgattccgc
       61 caaattccgc atggccttca atgtgccggc ctttcagtgg aataccgggc aggcgctcaa
      121 caacaccggc gagttgatta ctttggtcaa caacaccgac gccatcatgg acaccgtgcg
      181 ctattctgcc acagccatgc cctggcctgc atcggccaac ggcggcggcc cttcggtaca
      241 actctgcaat ccggcctccg acaacagcaa cggcgccaat tgggcggcct ccgtcgctcc
      301 taccggcttc tttatcaatg gcatagaagt gcnnnnnnnn nntggctact cccggcgcag
      361 ccaataattg cgcgcctccg atgcctgttt cttacccgcc ttacgaaatc ggagttgtga
      421 cgaccaacaa tgcgaatggc gtgaccgact cgctcaatgt gcgctgtcag ttgcagggta
      481 cggtatatgg catcaacctg agaacaacgg gattacagtt taccatgatt gacgatcaga
      541 acgacggcat tgcagtgttc agcactaccg ccgatttcgg ttatcaggtg gaagaaagca
      601 acgagatcat cattcggggc cgcatcacgc agttcaacgg tctgacgcaa attctccccg
      661 acactgtgat cgtagtagcg cagagcaacc cgctcattcc ggctaccacc gtcactcaat
      721 tggacgaaag cacggaatct caattggttc gcattgaaaa cctgactttg gtggaccctg
      781 ctcaatggac gaatgccggc accgggttca acgtggacgc caccaacgga accaacacct
      841 tccagattcg catcgtatcc accagtaata ttttcggcac acccgctcct gccggcactt
      901 tcaacctgac gggaatcggc gggnnnnnnn nnnnnnnnnn nnggcagttc gacagttcgt
      961 cgccctacac ggaaggctac cagattttcc cacgatattt atccgacata gacatcatca
     1021 acgcagtatc caatccggtg aaggtgaatc aaggcgttcg ggtgagtccc aatcctgctt
     1081 tgggggtcgt tttgatcaag tccgaatctg cttttgatgc tgtgcgagtg acggacgccg
     1141 ccggccggac ggtatattac aactggtttg cgcctgttac cgaactgaac ttagatacta
     1201 ctacttggaa acgaggcact taccatgtgg cagtgatgag cggaaaagct accagattgg
     1261 ttcaggtagt gaaggctaaa taagtcaatc tattattatt cgatcaggct gcgggaggtt
     1321 ttagcgcttc tccgcagcct gtttgtttgc agacaaagcg acgcaaaaaa attttaaaga
     1381 acaccccaaa ttttcaatct ttaggtgaaa gtttgagaaa agctctatat ttcgcctcca
     1441 agaaacacaa accaaacatt tatgaagcat tttcgactct tagccttttg cctcagcgcc
     1501 ttgtggatca atgcaataga cgcacaggac attcactaca cgctgttcaa tatgtcgcct
     1561 ttgacgctca atcccgcact caccggcgcg tatgaaggca cggccagaat cgggggcatt
     1621 taccgcgatc aatggtccaa tgttattgcg aaaggtttta aaactccggg cgtgtattta
     1681 gacgctccca tcattcgcgg ctttggaaaa aaagactggg tgggcgtcgg cgcgatcaat
     1741 gtcacggacg aggccggccg atttgatctg gagactacca tatcaggtct ttcattggct
     1801 taccatgctg ctgtcggcga taaatcggtt tttaccatcg gccttcaagg cgccagagtg
     1861 cagcgcaaag tgaacggcgc aagcccttat acgcgctttg aggatgctta tgatgttaat
     1921 actcagagtt ttactggtat tagcgctgat cgtggcgcta tccgtccgaa catcaactac
     1981 acagatatca acgcgggctt gttgttccgc actaagcttg cagacaagca atctttggag
     2041 atcggcgttt cggcggcaca catcaatgct ccccgctact ccatgagcga ttctacaggt
     2101 cgcaacgacc ccggcacacg tcgtccgctt cgtttagccg ctcatgctca atacattacg
     2161 ccgctgaatg aaaagtggga tctaatgcct acagtgctgt ttcagtccac cgcgggcgcc
     2221 ttcgaaggcg tggcacaggc ttgggccggg tacaacatca aaaaagatgt acgcatcaat
     2281 ctgggcgcgg gctatcgttt gggcgatgcg tttcaggtac tcatcggcat ggattacgaa
     2341 gacctgaaag tggcagccag ctacgacatg aacgtatctt cattggcgcc tgtgagcaaa
     2401 acagtgggcg gctttgaagt atcggcttgg tacatactca aaatgtataa aaagcccgat
     2461 gtgaaaccgg tcattttctg cccgcgtttt taatgttgtt ttccaaatac accagttcct
     2521 ttatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgc
     2581 ttatttcttt tcatcgcttt ttgttccatt gtttctgccc aacagctttg ggcgcaaccc
     2641 attcggaagt ccaattacga caaaatcgtt gagttggctt acgaaaaatt ggccgaaaag
     2701 gattattaca atgccattac caaatttgaa gaagcctacg aagagcgcga agacaaaacc
     2761 ttcctgccca ttttggccga cctcaactac ttagtccgcg actttgtaaa agccgagcgc
     2821 tactatgccc gcatttttcg ggccgataaa accaacgaat ttgccgccaa gcgctatttc
     2881 tatgctcgtt cgctcaagta caacggcaag tacgaagagg ccattcctga gtttcaaaaa
     2941 gtattggaaa cggcgaccaa agactctttg cgcaccttga ttcgcaatga aatttccggc
     3001 gctgaaatgg cgatggaact cggcattgcc aactccgacg gtaccatagg caagggagtt
     3061 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnng gtttgctcag gtattcaagt cgggcaaggg agatcgcggc
     3241 tggaaaaaac cggaaccctt gggtgaaaaa atcaaccgtc ccgatgcgca aatcatcaat
     3301 gtagcttttt ctccaaaaga taaccgagtt tacatcacgc agggcgtgct ggaaggcaat
     3361 gtgatttccg acgccaaaat atatgtggcc gccggtacag atggcgctgc caatgaggtg
     3421 aaaggtatca acggcaacta cattgccaag cacccggcag tgggagagtt gtttggccgc
     3481 gaggtattgt atttttcttc caatatgccg ggcggctttg gcggcttcga cctgtactac
     3541 gccacccgca ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnaac acgccgggca atgaagagac gccatttttc cgcgacggaa ctttgtattt
     3661 cagctccgac gggcacccca ctttcggcgg ttatgacttg ttctttaccg tttgggacgg
     3721 cacctcctgg agcgcgccgg ccaatatggg cgcaggctat aacaccagcg tggacgatca
     3781 gtctttgagc ctggatgcag agggatataa aggattcctc acttcgaacc gcgcaggcgg
     3841 gcgctcgctt cggagtaaaa cctgctgcga cgatatttat gcctttgaaa tcgcccgcat
     3901 ttcggccaat ctggtagtcg gcgtttttga cgatgccaaa aagccactca acggcacttc
     3961 catcggtctc atcgaaatgc nnnnnnnnnn aaaacgaaaa acctggcaag atagacagcc
     4021 gtaccaatgc caacggcaac cgttatgagt tcccgctggc gctggatatg ccctacaaaa
     4081 ttgtggtgag caaagagggc tatcaccccg attctaccac catcacaacg gccaacctga
     4141 aggaaaacaa aaccttcgag catcgtttct acctcaaagc caagccggtg ccgccgccgg
     4201 tgccggaata cgataccatc acgcaggaaa agccgattgt gttggagaat atcctgtacg
     4261 acttcgacaa ggatgtcatc aagccggaat cagagcctga tcttcaggta gtgtttgaat
     4321 tgatgacgga gtatcccgat atggtgattg aattagnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnncc agagagcgaa tcaacgctgt tggttatggg gagacggttc
     4501 cgcaaacggt cagcgccaaa gctgctgctc agagcaattt cctcaaagaa ggcgatgtac
     4561 tcactccgga atacattgac aaactggctt ccgaagagca aaaagaggcc gcacacgcgc
     4621 tcaatcgccg tacagagttc aagattgtga aaggcccaac aagcatcatc atcaagagta
     4681 cgcgcttgcg caaggtggag cctactacca aaacagcgcc caacaaacag tctcagcccg
     4741 ccgctgcgcc gaagcccgcc cagagcagcg ctccggtgat ggagttcaag gagaagtttg
     4801 ttgatttcgg ctcggtgaaa aagggcgaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnncg gtgaagccgg gcgaaaaggg catcatccat gtggtattcg
     4981 acagcacgga gaaggagaaa tccgaagtca ttgacgtgga catcttcctc gattataccg
     5041 atccggtgac ggaaatgccc gctatcgtgc gcctgcaata caagtttgac ctggtgaagt
     5101 gaggtttgtt tcctccaaga agaaaagagg cccggctttg gtgaaaagtc gggcctcttt
     5161 gttattattt ggcctcttcc tcctgccgtc ttttgatctc atcccgaatg agggcggccc
     5221 gctcgtagtc ttcgctgctc aacatatcct ccagcatttt ttgcaagccc tcgatagggt
     5281 aggaggacag ccctcctgat ttgnnnnnnn nnnnnnnnnn nnnnnnnntg cgggcgcgag
     5341 gctgcggctc ctcttcttcc agaatgatgc ccgcagtttc catgatgaac tcgtaggtgt
     5401 ggatggggca atggaaacgg gcggccaacg ctaatgcgtc ggaggtgcgg gcatctattt
     5461 cgtagagttc gccgtctctt tgacacacca tgcgggcgaa aaagataccg tctgtaagat
     5521 tgttgatgat aacttcctgg agatcaatgt gaaagatgtc catgaagttt ttgaaaagat
     5581 cgtgcgtcat g
//
LOCUS       DAORLF010000320         5564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_7947_length_5564_cov_2.252133, whole genome shotgun sequence.
ACCESSION   DAORLF010000320 DAORLF010000000
VERSION     DAORLF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_5564_cov_2.252133"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    167..347
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<348..>557)
                     /locus_tag="PK198_20155"
     CDS             complement(<348..>557)
                     /locus_tag="PK198_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF41121.1"
                     /translation="AKLERVANILKTIAHPMRLSIVHLLEKHPRLTVTEICDTLNSEQ
                     SLTSHHLQTMRLNGVLSVQREGRCMY"
     assembly_gap    558..730
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(795..>1160)
                     /locus_tag="PK198_20160"
     CDS             complement(795..>1160)
                     /locus_tag="PK198_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRF41122.1"
                     /translation="FYTIGQRKGLGMAFGEPMFVTEIRPETNTVVLGRLEELIRNGMT
                     VGQVNPMKYAQIPEGIEAVTRIRYKDRGTLSRLSNIGDDKVFVDFFANVSGVAPGQSA
                     VFYEGDDVIGGGVIYGNGK"
     assembly_gap    1161..1171
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1172..1899)
                     /gene="mnmA"
                     /locus_tag="PK198_20165"
     CDS             complement(<1172..1899)
                     /gene="mnmA"
                     /locus_tag="PK198_20165"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRF41123.1"
                     /translation="MSKHGKVLVAMSGGIDSTVTAMMMHEEGYEVVGITMKTWDYANS
                     GGSRKETGCCSLDSINDARQVAVTMGFHHFIVDIREEFGDYVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWSALLKRADALDCQFIATGHYAIIKEHEGRKFISKAVDDNKDQSYVLWG
                     LSQECLQRSQFPLGGFTKPQVRQMAADMGFLELSKKAESYEICFVPDNDYRSFLKRKV
                     EGLEERVAGGHFVNTEGKILGVHEG"
     gene            2410..2997
                     /locus_tag="PK198_20170"
     CDS             2410..2997
                     /locus_tag="PK198_20170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF41124.1"
                     /translation="MKKVLLSLAVLTLALAAAQAQPILKKGSVDFAAGVGLVPTYVAD
                     GAHINMLPVNVRLGYRISNNFSMSAFAAYSASEKNNVVRPDESIENIKNQDLTLGLRG
                     AVHAIRAERFDVYGGFMLGYNMPNTDITQVSGPKTETPLPGGLTPSFSRPATNKLTYS
                     GFIGASYLTGRNLGFFGEVGYGISLFNVGMQWKLR"
     gene            complement(3151..5283)
                     /locus_tag="PK198_20175"
     CDS             complement(3151..5283)
                     /locus_tag="PK198_20175"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF41125.1"
                     /translation="MKKTSERPKIDGDVTEAAWFTGQPARQFWQLFPTDSVRAPWDTE
                     IFMTYDDQFLYVAAKCYSKDSNFVIPSLRRDYRAGGNDNITFVFDTFRDRTNAIVFGM
                     NPLGVTREALISNGGVDPGRDFDEFWDNKWQGASNIHEGYWSCELAIPFSSLRFREGD
                     TEWFFNSYRFDMQANVQSTWNRIPQNQPIMSLGYMGKIIWDAPLRKPGANASIIPYVN
                     TGYAHRFVEDGVRIPDSRPEYNFNLGADAKIQISSGLNLDLTVNPDFSQVEVDQQVIN
                     LDRFEIFFPERRQFFLENADLFSSFGNGRINPFFSRRIGVTQDPSTGQNIQNPIYGGA
                     RLSGKLDNNWRIGLLSMQTAPNKNLDQPSTNYTVAAMQRKIFSRSNIAAMFVNKQSFA
                     RSDSSGSAPAFNRVAGLDYNMASSDNKLTGKIFYHRSFSPGSNEGAFAHGANMEYRVK
                     RWSAAWEHQWIGNGYNAEVGFVPRKNFFRISPNMQYFFYPGKGAFNQHSIGAEVSLLW
                     MPELGRTDHEYNLWWEGSMRNTGEMSAGISNQYIYLFEDFDPSGTGGRPLPAGTAYNF
                     TGVGGSFQSDRRPKLNYRIEPAFGQYFNGYAFNLEGSVSYRYQPYGAIAININYTAID
                     LPEPYSDASVVLIGPRIDLTFSKSVFLTTFIQYNQQIDNININARLQWRYAPVSDFFL
                     VYTDNYNALNISTKNRALVAKWTYWLNV"
BASE COUNT         1228 a         1298 c         1317 g         1356 t
ORIGIN      
        1 gcatgatttt cgctaaatct gttcctttaa gcacactatt tgtcaaagaa tcaaatttta
       61 aagccatgaa attcaaaacc aatatccatt gcagcaattg tgtgcggtcg gtcagcggct
      121 ttctcaacga ggcacaaggg gtagaacact gggaagtgga cacagcnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta catgcaacgg
      361 ccctcgcgtt gtacgctgag taccccgttg aggcgcatgg tttgcaggtg atgtgaggtg
      421 agcgactgtt cgctgttcag cgtgtcgcat atttccgtga cagtcaggcg ggggtgcttt
      481 tccaacaaat gcacgatact caggcgcatg gggtgcgcta tggttttcag aatattagca
      541 acgcgttcca atttggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn cacaggccta actgaggagg aagtattgca ggtattaaaa caaaatggac
      781 tgttgccctg agcgctattt cccgttccca taaataactc cgcccccaat cacgtcgtcg
      841 ccttcgtaaa agaccgccga ttggccgggc gctacgccgc tcacgttggc aaaaaaatcc
      901 acaaacacct tatcatctcc gatgttactc agtcggctca gcgtgccgcg gtctttgtag
      961 cgaatgcggg tcacggcttc aataccctcc ggaatctgtg cgtatttcat cgggttcacc
     1021 tggcccaccg tcataccgtt cctgatcagt tcttccaatc gccccaacac aacggtattg
     1081 gtttccggcc tgatctctgt gacgaacatc ggttcgccga aggccatgcc caagcccttg
     1141 cgttgcccga tggtgtaaaa nnnnnnnnnn nccctcgtgt acgcccagga ttttaccttc
     1201 cgtgttgaca aaatgacctc cggctacccg ctcttccagg ccttctacct tccgtttgag
     1261 gaaactccgg tagtcgttgt cgggcacaaa gcagatttca tagctctcgg cttttttcga
     1321 cagttccaaa aaacccatat cggcagccat ctggcgcacc tgcggcttgg tgaaaccacc
     1381 cagagggaac tggctgcgct gtaaacattc ctggctaagt ccccacagta catacgactg
     1441 gtctttgttg tcgtccacgg ctttggagat gaattttcgc ccctcatgct ctttgataat
     1501 ggcataatgg ccggtggcaa taaactgaca atcaagtgca tcggcccgtt tcagcagcgc
     1561 gctccacttg atatgcgtat tgcacaacac acagggattg ggggttcgtc cggccaaata
     1621 ttcatctacg aaattgtcta tcacataatc gccgaactcc tcccggatgt ccacaataaa
     1681 atgatggaac cccatagtta cggctacctg gcgcgcatcg ttgatggagt ccaagctgca
     1741 acagcctgtt tctttccggc ttccgcccga attggcataa tcccatgttt tcatggtgat
     1801 gcccaccacc tcgtagccct cttcgtgcat catcatggcg gtgacggtgc tgtctatacc
     1861 gccgctcatg gctaccaata ctttgccgtg cttgctcatt ccgtgcagat attttggaaa
     1921 agaagtacaa atttccgtgc ggatagtttt atcgcgcagt ggtttttgcc ctttcccgga
     1981 aggtaattac gcctgcggcc ccagcccggc aagctgccgg ctattcttgc catgactccg
     2041 gttcttacca tctcgcacgt taaatgacat tcagccgaca ataattctgg ccagccggct
     2101 caatttttcg acccggccat acgttttgaa ggaaattgaa agattttcag tatttttttt
     2161 cattgggaag ccctgattgg gttgggaata aaattttaca aaagttaaaa aagcaactat
     2221 gatttaaagt ttctacacct tatttggaat taaattcttt aacaattgtt tttatttcgt
     2281 taaaacggaa taaaattttg ttaaaaactt gacaaatcga agtaaccgac gtaactttgc
     2341 agcgtcatat tgatagttcc aaacaagccg aataattgct ttattaacta actaacaaat
     2401 ttgttaccca tgaaaaaggt gcttctttcc ctcgctgttc tgactcttgc cttggctgcc
     2461 gctcaagctc agcccatcct taaaaaaggt tctgttgact ttgctgccgg tgtaggtctt
     2521 gttcctactt atgttgccga cggcgcacac atcaatatgc tgcctgtgaa tgtccgtttg
     2581 ggctatcgca tcagcaacaa tttcagcatg tctgcttttg cagcctactc tgcttctgaa
     2641 aaaaacaatg tagttcgccc cgacgaatct attgaaaata ttaaaaatca agatcttacg
     2701 ctgggtttac gcggtgcagt tcatgccatt cgcgccgagc gttttgatgt atatggcggt
     2761 tttatgttgg gctacaacat gcccaataca gacattactc aagtgagcgg ccccaaaacg
     2821 gaaacgccgc ttcccggtgg tctgacgcct tctttcagcc gtccggcaac gaacaaactc
     2881 acttacagcg gatttattgg cgcgtcttat ctgacaggcc gcaacctcgg cttctttggt
     2941 gaagtcggct acggcatctc cctgttcaat gtagggatgc agtggaaatt gcgttgatct
     3001 gacagacagt ttacgcccac ccccaatcaa ccaaattttt gcgatgagtc gcttccctct
     3061 tgtgcggaag cggctcatcg ctttttaaga cagtccttaa tacattttac cctggcctga
     3121 ctatggagag ctaagcatgt taaagaaaca tcacacattc agccaatatg tccatttggc
     3181 taccaatgcc ctgtttttgg tgctgatatt gagtgcgttg taattgtcgg tataaactaa
     3241 gaaaaagtcg gataccggcg cataccgcca ttgcaggcgg gcattgatgt tgatgttgtc
     3301 aatttgctga ttgtactgaa tgaacgtggt gaggaacacg cttttgctga aagtgaggtc
     3361 aatgcgcggc ccgatgagca ctaccgacgc atcggaatat ggttccggaa gatcaatggc
     3421 tgtatagtta atattgatcg caatggcccc gtagggctga taacggtagc tgaccgagcc
     3481 ttccagatta aaggcgtaac cgttgaaata ctgtccgaaa gcaggctcga tgcggtagtt
     3541 gagtttgggc ctgcggtctg actgaaagga gcctcccacg cccgtgaagt tgtaggctgt
     3601 tcctgccggc aagggccgtc cacctgttcc ggagggatca aaatcctcga ataaatagat
     3661 gtactgattg cttatacccg cgctcatttc tcccgtattg cgcatactgc cctcccacca
     3721 aagattgtac tcatggtcgg tgcggcccag ttcgggcatc caaagcagag atacctccgc
     3781 accgatgctg tgttggttga atgcgccttt gccggggtag aaaaaatatt gcatattggg
     3841 gctgatgcgg aaaaaattct tgcgtggcac gaagcccacc tcggcgttgt agccattgcc
     3901 gatccattgg tgttcccagg ctgcggacca gcgcttcacc ctgtactcca tattggcgcc
     3961 atgcgcgaaa gcgccctcgt tggaacccgg cgaaaaagac cggtggtaaa aaatcttgcc
     4021 tgtgagtttg ttgtcagagg aggccatgtt gtagtctaac ccagccaccc tgttgaatgc
     4081 aggagcggag ccggaactgt cggaacgggc aaaggattgt ttgttgacaa acatggcggc
     4141 tatattcgag cgactgaaaa tcttgcgttg catggcggct acggtatagt tggtgctggg
     4201 ctggtcgagg tttttgttcg gggctgtttg catgctcagc agcccgatgc gccagttgtt
     4261 gtccaattta ccgctcaggc gggcgccgcc gtagatcgga ttctggatgt tctggcctgt
     4321 ggaggggtct tgcgtgacgc cgatgcgcct cgaaaaaaaa ggattgatgc gcccattgcc
     4381 gaaagaactg aacaggtcgg cattttctaa aaaaaactgg cgccgctctg ggaaaaatat
     4441 ctcaaaccga tctaagttga tgacctgttg atctacttct acttgcgaaa aatcggggtt
     4501 gacggtaagg tcgaggttga ggccggaact gatctgtatt ttggcatctg cgccgaggtt
     4561 gaaattgtat tccgggcggg aatcgggaat acggacgccg tcctccacga agcggtgcgc
     4621 gtagccggta ttgacataag gaatgatgga ggcattggcg ccgggcttgc gcaagggcgc
     4681 gtcccagatt attttgccca tataccccaa actcataatg ggctggtttt gtggaatgcg
     4741 gttccaggtg gactgcacat tggcctgcat atcaaagcgg tagctgttga aaaaccattc
     4801 tgtatcgcct tcgcggaatc ggagcgaact gaagggaatg gccaattcgc aactccaata
     4861 gccctcgtga atgttggagg cgccctgcca tttgttgtcc cagaactcgt cgaagtctcg
     4921 ccccggatcc acgccgccgt tggaaatgag cgcttcgcga gtgacgccga gcgggttcat
     4981 accaaatacg atggcgttgg tgcggtcgcg gaaggtatcg aatacgaaag tgatgttgtc
     5041 gttgcctccg gcccgatagt cgcggcgcag agaagggatg acaaagttgg aatctttgga
     5101 ataacacttg gcggccacat agaggaattg gtcgtcgtag gtcataaata tttccgtatc
     5161 ccagggcgct ctgacggagt cagtggggaa aagttgccaa aactgccgcg ccggttggcc
     5221 ggtaaaccag gcggcttccg ttacatcgcc gtctattttc gggcgttcgg atgttttttt
     5281 caaaaggatc ggcggttgaa attcaacagg ttgggcggac atcattgccg aaaggcaaca
     5341 gaaagcaaac aggggagcaa tcaggcgcat gtggtgtgac ttgtgttcaa tcaattgacg
     5401 cgcaagatag ctactttatg aggaggcatc gcttactttt tcataccttt gccacactat
     5461 ggccgacgag aacaccagcg tacacaatcc gcacgaccgc ttcttcaagt ccctgatgca
     5521 caatcgggag acggcgattg cttttttgga ggaatttttg ccta
//
LOCUS       DAORLF010000321         5532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8018_length_5532_cov_2.061347, whole genome shotgun sequence.
ACCESSION   DAORLF010000321 DAORLF010000000
VERSION     DAORLF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_5532_cov_2.061347"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK198_20180"
     CDS             <1..185
                     /locus_tag="PK198_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF41126.1"
                     /translation="QGRKVGFEMLLKHSLTHQDIANITCTSRQTVTLVLNELKKSDLI
                     HFNRGKILVRDLARLS"
     assembly_gap    208..389
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..1516)
                     /gene="mtaB"
                     /locus_tag="PK198_20185"
     CDS             complement(<390..1516)
                     /gene="mtaB"
                     /locus_tag="PK198_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRF41127.1"
                     /translation="MATPKTVSFYTLGCKLNYSETSSIGRQFEGAGFAEVGFEQGADV
                     YVINTCSVTDFADRKCRNIVRQALRHSPNAYVVVVGCYAQLKPEEIASIPGVDLVLGA
                     AEKFHILDYIEDLSKAPGKGMVRAGEVRQAKDFVHAFSFGDRTRSFLKVQDGCDYKCT
                     FCTIPQARGQSRSNTVESVVNDARSIAAMGVKEIVLTGVNIGDFGNGTEVIEGAKPKK
                     EALFIDLIRALDEVEGIERFRISSIEPNLCTDEIISFVAESQRFAPHFHIPLQSGNNK
                     QLAQMRRRYRRELYAERVAQIKSVMPHCCIGVDVIVGFPGETEEDFLETYRFIQELDI
                     SYLHVFTYSERANTPAADMPDAVLMDERKRRNKMLSILSEK"
     gene            1572..1805
                     /locus_tag="PK198_20190"
     CDS             1572..1805
                     /locus_tag="PK198_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41128.1"
                     /translation="MKKLLTLLIFFQLAHGYCAAQCGRPSSVTALYSNDMRVFISNSG
                     DMFRDGNNYGEFRFAAEGLHNYHFSSRALGGRH"
     gene            1792..3240
                     /locus_tag="PK198_20195"
     CDS             1792..3240
                     /locus_tag="PK198_20195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41129.1"
                     /translation="MAGIDPGGNLRGAAQDYIMGQTRSDFAAGPVNTEQSFDASYCQN
                     WNRIWSVARHEVEAHIIDFEDNQQIDNPISSLMEWPGKGNPFFEVRLGFPLPVISHDL
                     APFYDRNNDGIYNPLEGDYPRIPNLSIIPEQISWTVFNDVHRNISQSQFPSAGLEIHL
                     CTWALDCTDNTMLNRTIFMKYSFINRGTERLDSTYMGLWHNFEVGCFSDDYVGSSPQH
                     QAFFAYNSDPVDETHCITGESFFGAYPPAQAVSILNRPLHAFVYTSSPSMDPANPTAF
                     YNVLTGRHADGTPITAAGSGYNTAGPITRYAYHGDPRDADEWTMYDVQWGLPYRLARS
                     IGSVDLGTLNPGEQTAVELAYLFVREPGLGHLENVGAMYEQLEILREQYANGFSDNCA
                     QAILSSETEQQHPQAFAVFPNPAGDRLHFRFEGQRINSLELTTLSGHTALSFKGPFWD
                     AMDVDVSTLPAGMYFVKMEQNGAVIVKKLIKH"
     gene            complement(3424..>5532)
                     /locus_tag="PK198_20200"
     CDS             complement(3424..>5532)
                     /locus_tag="PK198_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRF41130.1"
                     /translation="EKWSKLPSVGFPTDSNAGRIGLAMFRTGGRRVLFAAIDNYNLRP
                     KKEDDADALSKDQLRKMGKDEFLKLPKYKVKDYLSSNGFPEKYNADKIIEMVKSDKLK
                     PSALVEYTEDANAQLLSSEVIGLEIYRSDDDGKTWKRTHTDLIDDMYSSYGYYFGQIR
                     VATYDVNKIFVLGVPVLRSDDGGKTFRSVNGDNVHADHHALWINPRRPGHIILGNDGG
                     INISYDDGESWIKCNTPPLGQFYAVAVDMAKPYNVYGGLQDNGVWYGPSNYRAGTEWH
                     DSGQYPYKSIMGGDGMQVAVDTRDNATLYTGFQFGNYFRLNTKTGDRRYITPKHELGE
                     SPLRWNWQTPIWLSTHNQDILYMGANRLYRSLNQGNDFQAISSDLTTGGKKGDVPYGT
                     LSSIHESPLRFGLLYTGSDDGLVHVSRDGGATWTNISAGLPKDMWVSRVIASAHSESR
                     VYVTLNGYRWDHFEPYLFSSDDYGKTWQKLGVNLPTEPVNVVREDPKNPDLLYLGTDH
                     GLYFSLNRGGSFMLLDNGLPATPVHDLVVHPRDAELVVGTHGRSLYVGRVKELQQLSA
                     ENQAKNLLVFEMDKMRASPRWGSIGWWNSTKPSASIPVYVRNAGKMSISIATESGIVL
                     KTWTAEADKGLNYIPYDLSIDASVLKSYNAWLNEKVKADEKPIVVKAAEDGRAYIYKG
                     AYTITVEKDGQSEKIKWTLE"
BASE COUNT         1198 a         1374 c         1435 g         1343 t
ORIGIN      
        1 atcagggcag gaaggtaggt tttgaaatgc tgctcaagca ctcgctcacg caccaagaca
       61 ttgccaacat cacctgcact tctcgccaaa cggtgacgct ggtactcaac gaattgaaga
      121 agtcagacct gattcacttc aaccggggca aaattctcgt tcgggacctg gcgcggctct
      181 cctgagaccc acactacgcc gccctctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcttctcgct cagaatgctc agcatcttgt
      421 tgcgccgttt ccgctcatcc ataagcactg catccggcat gtcggcagcc ggcgtgttgg
      481 cccgttcgga gtaggtgaaa acgtgcagat aggaaatgtc aagttcttga atgaagcggt
      541 acgtctccag aaaatcttct tccgtttctc ccggaaaccc cactataaca tccacaccga
      601 tacaacaatg cggcatgacc gacttgattt gggccacgcg ttcggcgtac agttcgcggc
      661 ggtagcggcg gcgcatttga gccaattgct tgttgttgcc cgattgcagc ggtatgtgga
      721 aatgcggcgc aaagcgttgt gattcagcca caaatgagat gatttcatcg gtgcaaagat
      781 tgggttcaat gctggaaatg cggaaccgct cgatgccctc cacttcgtct aaagcgcgaa
      841 tgagatcaat aaaaagtgcc tcctttttgg gtttggcgcc ttcgatgacc tctgtgccgt
      901 tgccgaaatc gccgatgttg acgccggtga gcacaatttc ttttacgccc atggcggcaa
      961 tgctccgggc gtcgttcacc acgctttcca cggtgttgct gcggctttgg ccccgcgcct
     1021 gtggaatggt gcagaaggta catttgtaat cgcagccgtc ctgcactttg aggaaagagc
     1081 gggtgcggtc gccgaaagaa aaagcgtgta caaagtcttt ggcctgccgc acttcgccgg
     1141 cgcgcaccat gcctttgcct ggcgctttcg acaggtcttc gatgtagtcg agtatgtgaa
     1201 atttttcggc ggcgcccagc acgaggtcaa cgccgggaat ggaggcaatt tcctccggct
     1261 tgagttgggc ataacacccc acgacgacca cataagcatt gggcgaatgc cggagtgcct
     1321 gccggacgat gttgcggcac ttgcgatctg caaagtccgt caccgagcag gtgttgatga
     1381 catacacgtc ggcgccctgc tcgaaaccga cctcggcaaa gcctgcgccc tcgaactgcc
     1441 gcccgatgga ggaggtctca gagtagttga gcttgcagcc cagggtataa aatgatactg
     1501 ttttgggtgt ggccattgat ggaaatttcg ccgcaaaggt aagattattg tgtaaccata
     1561 aaactcgaac aatgaaaaag ttacttactt tgttgatttt ctttcagtta gctcacggct
     1621 actgtgccgc ccaatgcggt cgtccatcct cggtcactgc tttgtacagc aacgacatgc
     1681 gggtgttcat cagcaattcc ggcgacatgt ttcgggacgg caacaactac ggtgagttca
     1741 ggtttgccgc tgagggtttg cacaactacc attttagctc aagggctttg ggtggccggc
     1801 attgatcccg gtgggaattt gcgcggcgct gcacaggatt atattatggg gcaaacgcgg
     1861 tcggattttg cagcggggcc ggtaaatacg gagcagtcat tcgacgcatc ttattgccaa
     1921 aactggaacc gaatctggtc ggtcgcccgc catgaagttg aagcgcatat cattgacttt
     1981 gaggataatc aacaaattga caacccgata tcctctctca tggagtggcc gggaaaaggc
     2041 aaccctttct tcgaggttcg cctgggcttt cccctgccgg taatttcgca tgatttagca
     2101 cctttttatg accgaaataa cgatggtatt tacaatcctt tggaaggaga ttaccctcga
     2161 attcccaacc tgagtataat accggaacaa atctcctgga cggtattcaa cgacgtacac
     2221 cgcaacattt cgcagtcgca gttcccatcc gccggcttag agatacacct gtgtacatgg
     2281 gcgttagact gtaccgacaa taccatgctg aacagaacga tttttatgaa atactccttc
     2341 attaatcgcg gtaccgaaag gctggattca acctatatgg ggttgtggca caattttgaa
     2401 gttggttgtt tttcagatga ttacgtcggg tcatcgccac agcatcaggc tttttttgcc
     2461 tataacagcg atccggtaga tgaaacgcat tgtataacag gagaatcctt tttcggagcc
     2521 tatccgcccg cgcaggcagt aagcattctg aaccgcccgc tgcacgcttt tgtatatacc
     2581 tccagccctt ctatggatcc cgcgaatccg actgcatttt acaacgtgct taccggtcgt
     2641 catgcagacg gcacgcccat tactgccgcc ggatcgggtt acaatacggc aggtccgatc
     2701 acccgctacg cgtatcatgg cgatccgcgt gatgcagatg aatggaccat gtacgatgtg
     2761 cagtggggct tgccgtatcg ccttgcccgt agtataggca gcgtggattt ggggacgctg
     2821 aatcctggcg agcagactgc ggtggaattg gcatatttgt ttgtgcggga accgggattg
     2881 gggcatttgg aaaacgtggg cgctatgtac gagcaattgg agatactgag agaacaatat
     2941 gctaatggtt tttcagataa ttgcgcacaa gccatactaa gctcagaaac agaacaacaa
     3001 catcctcaag cctttgcggt ttttcccaac ccggcaggcg accggcttca ttttcgtttt
     3061 gaaggacaac gcatcaacag ccttgaattg acaacacttt cggggcatac agcactttcc
     3121 ttcaaggggc cgttttggga tgcaatggat gtggacgtca gcacgcttcc ggcgggtatg
     3181 tattttgtca aaatggagca aaatggagct gttatcgtaa aaaagctgat aaaacactga
     3241 acgcgtattt taagtgaagg ggtgacgccc ccaagttcac cccttcacta aactaactta
     3301 gtagtggggc aaaaataaat gctcattttc tttaggccca aggattagat aagacactta
     3361 aaatgaaccg tttacttatc taatcctttg acgaaaatga tcactttatt tttttatcgc
     3421 cccttactcc agcgtccact ttattttctc cgattggccg tccttttcta cggtgatggt
     3481 gtaagcgcct ttataaatat aggctctgcc gtcctccgca gctttcacca cgatgggctt
     3541 ttcgtctgct tttacctttt cgttgagcca ggcattgtag gatttcagca cggatgcgtc
     3601 tatgctcaga tcgtaaggaa tgtagttgag tcctttgtct gcctctgctg tccaggtttt
     3661 gagtacgatg ccgctttctg tggcgatgct gatgctcatt ttgccggcat tgcgcacata
     3721 cacaggtatg gaggcggagg gttttgtact attccaccag ccgatgctgc cccagcgagg
     3781 actcgcccgc attttatcca tctcaaaaac aaggaggttt ttggcctgat tttcagcgct
     3841 caattgttgc aattccttta cgcggcccac atacagcgag cggccatgcg tacccaccac
     3901 caactcggcg tcgcggggat gcaccacgag gtcgtgtacc ggcgtagcag gcaggccgtt
     3961 gtccaacaac atgaacgagc cgccgcgatt cagagaaaaa tacagcccgt gatcggtgcc
     4021 gagatagagc agatcggggt ttttggggtc ctcgcgcact acgtttactg gctccgtagg
     4081 caggttgacg ccgagtttct gccaggtctt gccgtagtcg tcgctggaaa acaggtacgg
     4141 ctcgaagtga tcccagcggt aaccgttgag ggtaacgtac acccggcttt cggaatgagc
     4201 cgaggcaatg acgcgcgaca cccacatgtc cttcggcagc ccggcgctga tgttggtcca
     4261 ggtggcgcct ccgtcgcggc tgacgtggac gaggccgtcg tcggagccgg tgtagagcag
     4321 cccaaaccgc agcggcgact cgtggatgct gctgagggtg ccgtagggaa cgtcgccttt
     4381 tttgccgccg gtagtgagat cggaagagat ggcctggaag tcattgccct gattcagaga
     4441 gcgatagagg cggttggcgc ccatgtagag gatgtcctga ttgtgagtgg aaagccaaat
     4501 cggcgtttgc cagttccaac gcaggggcga ttctccgagt tcgtgtttgg gcgtgatgta
     4561 tctgcggtct ccggtttttg tgttgagccg gaagtagttg ccaaactgaa atcccgtgta
     4621 aagggtggca ttatcgcggg tatccacagc cacctgcata ccgtcgccgc ccatgatgga
     4681 tttgtaagga tattggccgc tgtcgtgcca ttcggtgccg gctctgtaat tggagggccc
     4741 gtaccaaacg ccgttgtcct gcaagccgcc atatacgttg taaggcttgg ccatgtccac
     4801 cgctacggcg taaaactggc ccagcggagg cgtgttgcac ttgatccagg actcgccgtc
     4861 gtcgtaagaa atattgatgc cgccgtcgtt gccgaggatg atatgccccg gcctgcgcgg
     4921 attgatccaa agtgcatggt ggtcggcatg aacgttgtcg ccgttgacgg agcggaaggt
     4981 tttgccgccg tcgtcggagc gcaaaacggg tacgcccagc acaaaaatct tgttcacatc
     5041 atacgtcgcc acccgaatct gaccaaaata atacccatac gaactgtaca tatcgtcaat
     5101 gaggtcggta tgggtgcgct tccaggtttt gccgtcgtcg tcggagcgat atatctccag
     5161 accgataact tcagaactca aaagctgcgc attggcatct tcggtgtatt ccactaaagc
     5221 agagggtttt aacttgtccg acttcaccat ttcaatgatt ttatcggcgt tgtatttttc
     5281 aggaaaacca ttggaggaca ggtagtcttt taccttgtat ttcgggagtt tcaaaaactc
     5341 gtctttgccc attttgcgca gttggtcttt gctcaaggca tccgcatcgt cttctttttt
     5401 gggacgcagg ttgtaattgt caatcgcggc aaacagcacc cggcggcctc cggtgcggaa
     5461 catggccaaa ccgatgcggc ccgcgttgga atcggtaggg aaacctacgg agggcagttt
     5521 actccatttt tc
//
LOCUS       DAORLF010000322         5531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8021_length_5531_cov_1.897188, whole genome shotgun sequence.
ACCESSION   DAORLF010000322 DAORLF010000000
VERSION     DAORLF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_5531_cov_1.897188"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK198_20205"
     CDS             <1..214
                     /locus_tag="PK198_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF41131.1"
                     /translation="RFHRRVELWGEGVRFTDLKRLNLPLNRNGISNQLLVMVAITDVP
                     AGDKQWQWLFHQDELNANPAIVQNPL"
     gene            523..>3049
                     /locus_tag="PK198_20210"
     CDS             523..>3049
                     /locus_tag="PK198_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF41132.1"
                     /translation="MKKLVLVFMLLLSGAAALTAQRAITGKVTDEKGEALIGASILVK
                     TTSVGTVTDLDGSFRLQAPAGATTLIVSYTGYVSKEVALSAASNYDITLSTDVVAISE
                     VVVIGYGTQQKREITGNIASIKGSDIANLAVQSFDQALQGRAAGVNVSIPNGVLNNPP
                     VFRVRGVNSISLSSFPLIVIDGVPTFSGDLSTSAAANNPLSNLNPADIESIEILKDAS
                     AAAIYGSRAAAGVVLISTKRGQSGKARVNYDGWASWASPVRLPDLLNAQEYVTLKNEA
                     ISNANQLSGTANPTPPQFFLDTINGQLVDTDWYDYAYRTGFSHNHALSFSGGSDKTTY
                     FLSMGFTNQQGMIIANDFERKSIRLNLDHKMGKFLSLGATAGYSNNINRAPNTGSLPG
                     AGFNTSGLGRIPLITAPNVSPYTATGAYNIRTDNTMGRNRNLTQSGFVNPVPIIDLNS
                     FSSESGQIQASVYAQANILTGLAFRSQYGIDQINVEDLTFWNPIHGDGFGQNGYALNT
                     SRKSNRWNWTNTLQYDFNLGETNNLSLLVGNEQQYTKTDRWGAARNQIADPFFTTYQG
                     NFTTINPANNFQGENYFLSYFARANYDFKKLILATFNVRQDEFSAFAPGKKRGVFWGA
                     SLGLTLSELDFWKNSIGNLFSFFRVRGSYGQVGNNNGIGDFASLSTYGSGLYGTNATL
                     AFSQAGNPDLTWETSKKTDVGINFGLWEDRITGEISYFLNDIDGLILAAPQSPSKGVP
                     GNSISTNVGRMQNKGVEIGLNGTILRTNKFSWTSNFNLTFMENEVLSLAAGNRDIRVA
                     TAGLELTNIIRVGESIGSILAVETPGVNPANGRRIFLDAQGRQV"
     assembly_gap    3050..3132
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3133..3669
                     /locus_tag="PK198_20215"
     CDS             <3133..3669
                     /locus_tag="PK198_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF41133.1"
                     /translation="TDGKIYGPTLPKWFGAWDHTFRFKSIDFNVQAQFSGGNYIYNGT
                     QAGMRDQRFWNNHTDVLNRWQNEGDVTDIPRLVWLDNVSNGSAFPVSSNVEKGDFLRI
                     RNITLGYTFPTNDLFKKASISNFRVYASVNNAFLFTGYSGTDPEVSTNGNDPGSAGVD
                     RNTVPMARTILVGLNLGF"
     gene            3693..>4262
                     /locus_tag="PK198_20220"
     CDS             3693..>4262
                     /locus_tag="PK198_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF41134.1"
                     /translation="MKNIMNQFHFKVAAALVLLTGVFVSSCDKDKLNPEPLTFFSDLA
                     VFSDPSRILAQVNGMYASAKSGQFLGGRYYIYQDIRGEEFLNELTNGVTGLQTWNFTV
                     NDGSNEVNNFWNAAYAAINGANVFIEGLEANKAVLNNDALANNYLGEARFLRALSYYS
                     LLTLYSRPFTDGNGAKLGVPLRVKAEKGPA"
     assembly_gap    4263..4272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4273..5175
                     /locus_tag="PK198_20225"
     CDS             <4273..5175
                     /locus_tag="PK198_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF41135.1"
                     /translation="NNDLERSTVAAVYEQILGDLNFAETNLPNNYSSAALNTTRAHKN
                     TAIALKTRVLLSMGRYADVVTEGNKLVPATAPFKAASGVAHELQADITNVFKTPYTTT
                     ESIFSMPFTENNLPGTQNGLGGYYNPGPRGAGDYSLNPAAIIGDPAFAATDARRAWVF
                     VATNNKPYLNKFPTGPVHTDSAPVIRYSEVLLNLAEANARTSGVSQRAVDLLNAVRGR
                     SNPAGTYTTASFANADALINAILTERRIELLGEGFRSIDIMRLGQTFPAKASVTAVPP
                     SSNAYIWPIPSGELSVNALCVPNP"
     gene            5304..>5531
                     /locus_tag="PK198_20230"
     CDS             5304..>5531
                     /locus_tag="PK198_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF41136.1"
                     /translation="MTKTAAQRRLFTVSDYYKMAEAGILSPDDRVELIRGEILKKWRK
                     SSQHAGTVNHLAKLAILALHEKAVVSVQHPMS"
BASE COUNT         1340 a         1468 c         1301 g         1329 t
ORIGIN      
        1 gcggttccac cgccgcgtag agctttgggg cgaaggcgta cgctttaccg acctgaagcg
       61 tctcaatctg ccgctcaacc gcaacggcat ttccaaccaa ttgctcgtga tggtagccat
      121 caccgatgta ccggcaggcg acaagcagtg gcagtggctg ttccaccagg atgagttgaa
      181 tgccaacccg gccattgtgc agaatccgtt gtaggatttc ttagctgcga gctacgagcc
      241 gcgagcttcg agtttgagtt ctgtaaagat tgaaaattta cttaacatag cgggcattcg
      301 gaacgacggt ttcggatgcc cgttttttat gctgcgattc gactgaaaat atcaaatcat
      361 ggcaccatca ggcagcaaaa agcccggttg gaacgatata cattggctgc aaaaaaaact
      421 atggcagcat tggtttttta taaaaatggt acaaactgac gaatgttagt tttacctttg
      481 cgactaagga ttttctattt acaaaccaaa atacagtcag acatgaaaaa actcgtatta
      541 gtgttcatgc ttttgctgag cggcgctgcg gcgctgacgg cacaacgcgc gatcaccggt
      601 aaggtgactg acgaaaaagg agaagccctc atcggcgcct ccattctcgt caagacgacc
      661 tctgtcggta cggtcaccga tctggacgga agtttccgtt tgcaggctcc ggccggcgcc
      721 actacgctca tagtcagcta caccggctat gtgagcaaag aagtagccct ttcggctgca
      781 tccaactacg acatcaccct gagcaccgac gtggtggcca tctcagaagt agtggtgatc
      841 gggtatggta cgcagcagaa gcgggaaata acgggcaata ttgcctccat caaaggctcc
      901 gacatcgcca atttggccgt acagagcttt gatcaggcat tgcaaggccg tgcggcaggc
      961 gtaaacgtca gcattcccaa cggtgtgttg aacaacccgc ctgtgtttcg tgtacgcggt
     1021 gtgaactcca tcagtttgag ttctttcccg ctcatcgtga ttgacggagt acctaccttc
     1081 tccggcgacc tttctacatc ggctgcggcc aacaaccctt tgtcgaacct gaatccggca
     1141 gacatcgaga gcatcgagat tctcaaagat gcctctgctg cggccattta tggttccaga
     1201 gcggctgccg gcgtggttct catcagcacc aagcgcggcc aatcgggcaa agcgcgcgtc
     1261 aattatgatg gttgggccag ttgggcctct cccgttcgtc ttcccgattt gctcaacgca
     1321 caggaatacg ttacgttgaa gaacgaagcg atcagcaacg ccaaccaact ttcgggtact
     1381 gccaatccga cgccgccaca attcttcctt gacaccatca atggccaact ggtagatacc
     1441 gactggtacg actatgctta tcgcacgggc ttttctcaca accacgcgtt gagtttctcc
     1501 ggcggtagcg acaaaaccac ttacttcctg tctatgggtt tcaccaacca gcagggtatg
     1561 atcatcgcca acgatttcga acgtaagtct attcgtttga acttggacca caaaatgggt
     1621 aagtttttga gcctcggtgc tacggccggt tattccaaca acatcaaccg cgctcccaac
     1681 actggctctc tgcccggtgc aggcttcaac acttcaggtt tgggtcgtat ccctctgatt
     1741 accgcgccca acgtatctcc ttacaccgca accggcgcct acaacatccg cacagacaac
     1801 accatgggac gcaacagaaa cctgactcaa tcaggttttg tgaatccggt acccatcatt
     1861 gatctcaata gcttttcttc tgagtccggc cagattcagg cctctgtata tgctcaggcc
     1921 aatatcctga caggactggc attccgctct cagtatggta ttgaccaaat caatgtggaa
     1981 gacctcacct tctggaatcc tattcatggc gacggtttcg gccagaatgg ctacgcgttg
     2041 aatacatcac gcaagagcaa tcgctggaac tggaccaata cgctgcaata tgatttcaat
     2101 ttgggagaaa ccaataacct ttctcttttg gtaggcaatg agcagcaata cacgaagacc
     2161 gaccgctggg gagctgcgcg caaccaaatt gcagatccgt tcttcacaac ctatcagggc
     2221 aacttcacga ccatcaaccc tgccaacaac ttccagggtg aaaactattt cttgtcttac
     2281 tttgctcgtg ccaactacga tttcaagaag cttattctcg ctacgttcaa cgtgcgtcag
     2341 gacgaattct ccgcttttgc tcccggcaaa aagcgcggtg tgttctgggg tgcttctttg
     2401 ggtctgaccc tgtcagagct tgatttctgg aaaaacagca tcggcaacct gtttagcttc
     2461 ttccgcgtac gcggcagcta cggtcaggta ggcaacaaca acggtatcgg cgattttgcc
     2521 tctttgtcca cttatggttc tggtttgtac ggcaccaatg caacccttgc attcagccag
     2581 gcaggcaacc ccgatctcac ttgggaaacc agcaagaaga ccgacgtagg tatcaatttc
     2641 ggcttgtggg aagaccgcat cacgggtgaa atttcttact tcctcaacga cattgacggc
     2701 ttgatcctcg cagcaccgca atctccttcc aaaggtgttc cgggcaactc tatctcgacc
     2761 aacgtaggcc ggatgcaaaa caaaggtgta gaaattggtt tgaatggtac gatcctgaga
     2821 actaacaagt ttagctggac gtccaacttc aaccttactt tcatggaaaa tgaggtgctc
     2881 tctctggccg caggcaacag agatatccgc gtagctactg ccggcttgga acttaccaac
     2941 atcatccgcg taggtgaatc catcggtagc attctggcag tggaaacacc tggagtaaat
     3001 ccggccaacg gacggagaat atttttagac gctcagggcc gtcaggttcn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnactgatgg taaaatttat ggtcctaccc ttcccaaatg gtttggtgca
     3181 tgggatcaca ccttccgttt caaatctatt gattttaacg tacaggcaca attctcaggc
     3241 ggcaactata tttacaatgg tactcaagcc ggtatgcgcg atcagcgttt ctggaacaac
     3301 cataccgatg tactcaaccg ctggcaaaat gaaggcgacg tgaccgatat acctcgtttg
     3361 gtttggttgg acaacgtctc caatggttct gcgttcccgg tatcttccaa cgtagaaaag
     3421 ggcgacttcc tgcgtatccg caacatcacg ctgggttaca ctttcccgac gaacgacctt
     3481 ttcaaaaagg ccagcatcag caacttccgc gtgtatgctt ctgttaacaa tgccttcctc
     3541 tttacaggct attccggtac cgacccagag gtttctacca acggaaacga ccccggatca
     3601 gcaggcgtag accgcaacac tgtgccgatg gcacgtacca tccttgttgg tttaaacctg
     3661 ggtttctaat catctaaaaa aagagagaca atatgaaaaa tatcatgaat caatttcatt
     3721 tcaaagtagc ggccgcactc gtcctgctta cgggcgtgtt cgttagctcc tgcgataagg
     3781 acaagctcaa tccggagccg cttactttct tttccgatct ggctgtattt tctgatccca
     3841 gccggattct ggcacaagtc aacggcatgt atgccagtgc gaaaagcggg cagtttcttg
     3901 gaggtcgtta ttacatttat caggacattc gcggagagga atttctcaac gaattgacca
     3961 atggtgtaac cggcctgcaa acctggaact tcaccgtcaa tgacggcagc aacgaggtaa
     4021 acaacttctg gaacgctgcg tacgctgcca tcaacggcgc caatgtgttc attgaaggtc
     4081 ttgaagccaa caaagcggtg ttgaacaacg atgctttggc caacaactac ctgggtgaag
     4141 ctcgcttttt gcgcgcactg tcatactaca gcctgctgac gctgtacagc cgtccgttca
     4201 cggatggcaa cggcgccaag ttgggtgtgc ctctccgcgt gaaagctgag aaaggcccgg
     4261 ctnnnnnnnn nnaataacga tctggagcgc agcacagtgg ctgccgttta tgagcaaatt
     4321 cttggcgact tgaactttgc tgaaaccaac ctgcccaaca actattcttc tgctgccctc
     4381 aacacgacgc gtgcgcacaa gaacacggct atcgcgctca aaacgcgcgt attgctgtct
     4441 atgggccgtt atgcagatgt agttacggaa ggcaacaaat tggtgcccgc tactgctcct
     4501 ttcaaagctg cgagcggtgt ggcacatgaa ttgcaagccg atattaccaa cgtattcaag
     4561 acgccgtaca ctaccacgga gagcatcttc tctatgccgt ttacggaaaa caaccttccg
     4621 ggtactcaaa acggtttagg tggctactac aatcctggcc cgcgcggcgc cggcgattac
     4681 tcccttaacc cggctgctat cattggcgac cctgctttcg cagcaacgga tgcaagacgt
     4741 gcatgggtat ttgtggctac caacaataag ccgtacctga acaaattccc taccggcccc
     4801 gtgcataccg actctgcgcc ggtgattcgc tactccgaag tgctcctcaa tttggcggaa
     4861 gccaatgctc gtacctccgg cgtttctcaa cgcgcagtgg acttgctcaa cgctgtacgc
     4921 ggccgctcta atccggcagg cacttataca acagccagtt ttgccaatgc agatgcattg
     4981 atcaacgcta ttctcaccga gcgccgcatt gaattgcttg gcgaaggatt ccgctctatt
     5041 gatatcatgc gtttgggcca aaccttcccg gccaaagcat ctgttacagc agtaccgccc
     5101 agctcgaatg cctacatttg gccgatcccg agtggtgaat tgtctgtaaa cgcgctgtgc
     5161 gtgcctaatc cgtaatttga accagggtta aaattggaat tttctaaaat cggagccgcc
     5221 tcgcacgtcg gggcggctct ttctttttgt acagttctga aaaatctcct gtatctttgc
     5281 ctatcatatt tgaaaactca agcatgacca aaactgccgc gcagcgccga ttatttactg
     5341 tttccgacta ctacaaaatg gcggaggcgg gtattctgtc gcccgatgac cgggtagagt
     5401 tgattcgtgg agaaatattg aaaaaatggc gcaagagcag ccaacatgca ggaacggtca
     5461 atcatttagc caagttggcg attttggcgt tacatgaaaa agctgttgtt tccgttcagc
     5521 acccgatgag c
//
LOCUS       DAORLF010000323         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8037_length_5526_cov_1.930908, whole genome shotgun sequence.
ACCESSION   DAORLF010000323 DAORLF010000000
VERSION     DAORLF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_5526_cov_1.930908"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..>325)
                     /locus_tag="PK198_20235"
     CDS             complement(130..>325)
                     /locus_tag="PK198_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRF41137.1"
                     /translation="DIFDKLDAERVSSLLQLLHHGDFGQVFITDTDEHRVENIIREFD
                     ADYRRYRIENGSAHLQNAIA"
     assembly_gap    326..360
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<361..>1221)
                     /gene="recF"
                     /locus_tag="PK198_20240"
     CDS             complement(<361..>1221)
                     /gene="recF"
                     /locus_tag="PK198_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568796.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRF41138.1"
                     /translation="QFKNYAGQTLELSERLNCFTGLNGMGKTNLLDAVYYLCLSKSRF
                     GLADAHLCRQGEDFFRLEGHFRWQQKPVKIVAKVQPRKRKVLENNDVAYSTLSEHIGL
                     FPVVFIGPDDTQMITEGSEERRRFIDNTLSQTDPLYLRQLMTYNQVLQQRNAALKQLA
                     EQRRFDQALISVYDAQLAPAGRYIHQKRREFVAEFAPIMQATYNKICGSRETVSCSYR
                     SALEQRPLEELLTQSIEKDRILQRTTEGVHRDDLELLIHDLPVKRFGSQGQLKSFVLA
                     LKLAQYDFLEK"
     assembly_gap    1222..1252
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1388..>1592
                     /locus_tag="PK198_20245"
     CDS             1388..>1592
                     /locus_tag="PK198_20245"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF41139.1"
                     /translation="MNTLAQLLKNARQKSGLKLREAAAIAQMDAALLSKFENGQRLPP
                     EDQAPALAKAYHLDEASLRRSILA"
     assembly_gap    1593..1612
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1613..>2110
                     /locus_tag="PK198_20250"
     CDS             <1613..>2110
                     /locus_tag="PK198_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41140.1"
                     /translation="VAVLLQYETGVHELLMVAESRIEYLRSERSLILPEIPGVLMDKL
                     IAIDALKTTWQACKPMNKSQLERMRQYFHTEYTFESNRIEGNTLTLQETSLVINEGLT
                     ISGKTMREHLEAVNHADAVAFITELATQQADLDRRTLLDLHNLILRGVDSENAGRYRS
                     VPVRI"
     assembly_gap    2111..2129
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2130..2476
                     /locus_tag="PK198_20255"
     CDS             <2130..2476
                     /locus_tag="PK198_20255"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF41141.1"
                     /translation="QPYLLDKLMEDYFIHYRQQRDTLHPVLLAADMHERLVSIHPFID
                     GNGRTSRLVMNLILLRNGYTIANLKGDPNSRLAYYRALEKVQADNEPEHFYTLVADAV
                     ETSLKAHIELAG"
     gene            2644..>3114
                     /locus_tag="PK198_20260"
     CDS             2644..>3114
                     /locus_tag="PK198_20260"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRF41142.1"
                     /translation="MSKITIEIWSDVMCPFCYIGKRQLERALREFPQGEAVEVIWKSF
                     QLQPNLQTDPSKSVLQSLAESKGWSREQTLSAMAHVTEMAAAEGLHYDFERAVVANSF
                     DAHRLTHLAKAHGLQNAVEEGLFAAYFCEGKNTADAEVLRSIGLSAGLPPDAVDA"
     assembly_gap    3115..3147
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3458..3504
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3505..3695
                     /locus_tag="PK198_20265"
     CDS             <3505..3695
                     /locus_tag="PK198_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF41143.1"
                     /translation="LNPSENYGQVYIRTNDIDQMYALAERNAVVHPNGRLATKPWGQK
                     EFSVLDPDHNLLTFGEDA"
     gene            complement(3662..4024)
                     /locus_tag="PK198_20270"
     CDS             complement(3662..4024)
                     /locus_tag="PK198_20270"
                     /inference="COORDINATES: protein motif:HMM:NF022643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HRF41144.1"
                     /translation="MTIRAFTPLMWIYLLLAIAGLIVPWYFNLQHLLYSPVAFTVAEF
                     FRQNTDTPMASSLTYDFLIGGNAAIIWMIVEHRRLKMKHLWLYIIATFLIAFAFTFPL
                     FLFQRERHYASSPKVNKL"
     gene            complement(4209..>4476)
                     /locus_tag="PK198_20275"
     CDS             complement(4209..>4476)
                     /locus_tag="PK198_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRF41145.1"
                     /translation="LAPLHDGSLLHLHKLAAGWDPTDRKSVIDLLQTAKSAGDIPTGL
                     LYLDMNTRNLHDLMKTAKKPLNSMNESELCPGGAVLEGINAGFR"
     assembly_gap    4477..4500
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4501..5270)
                     /locus_tag="PK198_20280"
     CDS             complement(<4501..5270)
                     /locus_tag="PK198_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRF41146.1"
                     /translation="MSFIKPQFHHPALPKNKVGYTKADYEGSISTLCAGCGHDSISAA
                     IVQACYEIDLEPHKLAKISGIGCSSKTPAYFLNNSHGFNSVHGRMPSVATGAYMANRD
                     LIYLGVSGDGDTASIGMGQFVHAVRRNLNMVYIVMNNGCYGLTKGQDSATADVGSINK
                     EGSENPFQGIDLCSLAIELGATFVARSFSGDKHQLVPLIKAAMSHPGFAFLDVISPCV
                     TFNNNKGSTKSYDYVREHIDATSELDFVPIHSEITIDY"
BASE COUNT         1240 a         1401 c         1466 g         1210 t
ORIGIN      
        1 ctgctatcac ctgccccatc acctgcgccc agacgtcgcg cacccgcgtt tcgtccaatt
       61 tggatttgag gcggtaggct tccagcattt ccgaaagcac ctccttcacg ctttgttcgt
      121 ttgtttttct catgcgatag cgttttgcag atgagcgctg ccgttttcaa tgcggtagcg
      181 gcggtaatcg gcgtcaaatt cccggatgat attttccacc cggtgctcat cggtgtcggt
      241 gatgaatacc tgcccgaaat cgccgtgatg cagcagttgc agcaggctgc tcacccgttc
      301 ggcatccaat ttatcaaaaa tatcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 cttttccaaa aaatcatact gcgccagttt cagcgccagc acaaaggact tgagttgccc
      421 ctgcgagccg aaccgcttca ccggcaggtc gtggatcagc agttccagat cgtcccggtg
      481 tacgccttcg gtggtgcgtt gcaaaatgcg gtctttttca atgctttgcg tcagcagttc
      541 ctccagtggg cgctgctcca gtgctgagcg gtagctgcac gatacggttt cccggctgcc
      601 gcaaatcttg ttgtaagtgg cctgcatgat gggcgcaaat tcggccacaa actcccgccg
      661 cttttggtga atgtagcggc ccgccggcgc caattgcgca tcatacacac tgatgagcgc
      721 ttggtcgaaa cggcgttgct cggccaattg cttgagggcg gcgttgcgct gttgcagcac
      781 ctgattgtag gtcatcagtt gccgcaggta cagcggatcg gtttgcgaaa gcgtgttgtc
      841 tataaacctc cggcgctcct cgctgccctc ggtaatcatc tgcgtgtcgt ccggcccgat
      901 gaacaccacc ggaaacaggc cgatgtgttc gctcagcgta gagtaagcaa catcgttgtt
      961 ttccagcact ttgcgctttc ggggttgcac tttggccact atttttaccg gcttttgctg
     1021 ccatcggaaa tgcccctcca agcgaaagaa gtcttcgccc tgccggcaca aatgcgcatc
     1081 ggcgaggcca aaccggctct tgctgaggca caggtaatac acggcgtcca agaggttggt
     1141 ttttcccatg ccgttcagcc cggtaaaaca attgagccgc tcagacaact ccagcgtttg
     1201 ccctgcgtaa ttcttgaatt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgcaaat
     1261 ccgtcggctt gttgaaacgt tgcaaaggta gcccgtttgg acaaaaatgg aagaaaaaga
     1321 gaaaacagga aacaaataac tggaacaaag tgttcttgac taaatttgtc aagaaaaaaa
     1381 taaaaacatg aacacattgg ctcaactcct caaaaacgcc cgccagaaat ccggcctcaa
     1441 actccgggaa gccgccgcca tcgctcaaat ggacgctgcg ctgctcagta aattcgagaa
     1501 tggccaacgc ctgccgcccg aagatcaggc gcccgcattg gccaaagcct accatttaga
     1561 cgaggcaagc ctccgccgca gcatcctcgc cgnnnnnnnn nnnnnnnnnn nnaagtagcc
     1621 gtactgctgc aatacgaaac gggtgtacac gaactgctca tggtagccga aagtcgcatc
     1681 gaatacctcc gcagtgagcg cagcctcata ctgcccgaaa tacccggcgt actgatggat
     1741 aaactcatcg ccattgatgc gttgaaaaca acatggcagg cttgcaagcc aatgaataaa
     1801 agccagttgg agcgcatgag gcagtatttt catacagaat acaccttcga aagcaaccgc
     1861 atagaaggca ataccctcac acttcaggaa acctcattgg tcatcaacga aggactcacc
     1921 atcagcggca aaaccatgcg cgagcatctg gaggccgtca accatgccga tgcagtagcc
     1981 ttcatcaccg aattggccac ccaacaagcc gacctcgacc gccgcaccct gctcgattta
     2041 cacaacctca tcctccgggg cgtggactcc gaaaacgcag ggcgctaccg ctcggtgccg
     2101 gtgcgcatcg nnnnnnnnnn nnnnnnnnnc acagccttac ttgttggaca aactgatgga
     2161 ggactacttc attcactacc ggcagcagcg ggatacattg caccctgttt tgctcgctgc
     2221 cgacatgcac gaacgccttg tgagcattca cccgttcatt gacggcaacg gccgtacctc
     2281 gcgcttggtg atgaacctca ttctgctgcg caacggctac actattgcca acctcaaagg
     2341 cgatccaaac tcacggctgg cgtactaccg cgctttggag aaagtgcagg ccgacaatga
     2401 gccggagcac ttttataccc tcgtagccga tgcggtagaa acctcgctga aagcgcacat
     2461 cgagctggcg gggtgaagtg tcgccggctc ctttttcaaa ataacctgta cctttggcgg
     2521 cgcttatcac aatgggggct tggcggacac gtttcgcgaa tgctcgcagt tggccatcgg
     2581 cacaacttgt cccgatgacg aaggaatcgg gattagcata ttatcaaatc agcacattga
     2641 cttatgagca aaataaccat agaaatctgg tcggatgtga tgtgtccgtt ctgttacatc
     2701 ggcaagcggc agttggaacg cgcactgcgg gagtttccac aaggcgaagc ggttgaagta
     2761 atctggaaga gttttcaact tcaacctaat ctgcaaaccg acccctccaa aagcgtgctg
     2821 caatcgttgg ccgaaagcaa gggctggagc agggagcaaa ccctctctgc catggcgcat
     2881 gtaaccgaga tggccgctgc cgagggtctg cattacgatt ttgagcgggc tgtggtggcc
     2941 aattcgttcg atgcgcaccg actgacgcat ctggcgaaag cgcatggact gcaaaacgcc
     3001 gtggaagaag ggctttttgc ggcttatttc tgcgagggca aaaacacagc cgatgcggaa
     3061 gtgctgcgct ccatcggcct gtcggcgggc ttgccgcctg atgcagtgga tgctnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ccacgatatt tacgaagccc ggcaggtggg
     3181 cgtaagcggg gtgccgtttt ttgtattcaa tgatcgtctg gcggtgtccg gggcgagggg
     3241 cagcgaggtg tttttgcagg tgctggggga ggtggctaag aattgaataa tcaattactt
     3301 gaatcaacaa agacctgaaa aaatgctcac ctccatccat cccaaattgc cgatgcgtaa
     3361 caaagccgtc acgcgggatt tttacctcaa caaattagga tttgaagaat tcggcgcggc
     3421 cgattacgcg ggttatctga tgctccaaaa agatcagnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnaactca atccgtcaga aaactacgga caggtgtata
     3541 tccgaaccaa cgatatagac caaatgtacg cattggctga acgaaacgcc gtcgtgcatc
     3601 cgaatggccg cttggcaaca aaaccctggg gccaaaagga gttttcagtc ctcgatcccg
     3661 atcacaattt gttgaccttc ggggaagatg cgtagtgccg ctcccgttga aacaaaaaca
     3721 gcggaaaggt aaacgcaaac gcaatgagaa aagtagcgat gatgtagagc cacaagtgtt
     3781 tcattttgag cctcctgtgc tctacgatca tccagatgat ggcagcattg ccgccgatca
     3841 gaaaatcata agtgaggctg gaggccatcg gggtatctgt gttctgccgg aaaaactccg
     3901 ccacggtaaa agctaccgga ctgtacagca aatgctgcaa attgaaatac cagggcacga
     3961 tcagcccggc tatggccaag agcagataaa tccacatcag tggggtgaat gcccgtattg
     4021 tcatactttc tgattttaga atgcttttca aaacccattt tgaatagaaa tggcagaaag
     4081 cgtgttttgt ttgcatggct gccggttctc cccaaccggg gagttagtgg ggcgagcgca
     4141 gggcaaacaa aaccgctcgc gcgaagttct acttcgtgct ccgctcgcgc gaagttctac
     4201 ttcgcgtgct atctaaaccc cgcattgatc ccctccagta cggccccgcc cggacacaat
     4261 tcgctctcgt tcatgctgtt gagtggcttt ttggcggttt tcatcaggtc gtgcaggttg
     4321 cgggtattca tgtccaaata gagcagacca gtgggaatat cgccggcgga tttggccgtt
     4381 tgcagcaggt caatcaccga tttgcggtcg gtggggtccc agccggctgc gagtttgtgc
     4441 agatgcaaga gagagccgtc gtgcaggggc gccagtnnnn nnnnnnnnnn nnnnnnnnnn
     4501 tcatagtcaa tcgtgatttc cgagtgtatc ggcacgaaat ccagttccga agtggcgtca
     4561 atgtgttcgc ggacataatc ataagatttg gtagaaccct tgttgttgtt gaaggtcacg
     4621 cagggcgaga tcacgtccaa aaacgcaaag cccggatgcg acatggcggc cttgatgagc
     4681 ggcacgagct ggtgtttgtc gccggaaaag ctgcgggcca cgaaggtagc gcccagttcg
     4741 atggcaagac tgcacaggtc tatgccctga aaggggtttt cgctgccctc tttgttgatg
     4801 gaccccacat cggcggtggc ggaatcctgg cctttggtga ggccgtagca gccgttgttc
     4861 atcacgatat agaccatgtt gaggttgcgc cgcacagcgt gtacaaactg ccccatgccg
     4921 atggaggccg tatcgccgtc gccggaaacg ccgaggtaga tcaggtcgcg gttggccatg
     4981 tacgcgccgg tggcgacgga gggcatgcgc ccgtgtacgg agttgaaacc gtgggagttg
     5041 ttgagaaaat aggccggcgt tttgctcgaa cagccgatgc cggatatttt ggccaatttg
     5101 tgcggctcca gatcaatttc ataacaggct tgcacaatgg cggcgctgat ggagtcgtgg
     5161 ccgcagccgg cacagagcgt ggagatgctg ccttcgtagt cggccttggt gtagcctacc
     5221 ttgtttttcg gcagtgccgg atggtggaat tgtggtttta tgaaggacat gttgtgtgtt
     5281 ttttttagat ggatgatttt tgggtgagct tcaaccacgc tcgaggcgtg gtgcaagcct
     5341 caagctgtgt tttgctaagg tgatgcttgt tcgttcattt gggtgagggt gttttggagg
     5401 aaggcttcgg ggtcttcgcc ccaggcggct ataacttgca tggttgcctg aaggtagttc
     5461 tgcacggaag gcaggagctc cacaaaaggc catcctgcac atatggcttc tgcacagtag
     5521 gccagg
//
LOCUS       DAORLF010000324         5513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8055_length_5513_cov_2.152254, whole genome shotgun sequence.
ACCESSION   DAORLF010000324 DAORLF010000000
VERSION     DAORLF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8055_length_5513_cov_2.152254"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..967)
                     /locus_tag="PK198_20285"
     CDS             complement(122..967)
                     /locus_tag="PK198_20285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41147.1"
                     /translation="MKTWMLISLLGLMPLALNAQTFTEEINLPCGDTSVIGSLGYPTW
                     NPPQIVKRASYGHSSLLGDFIEANILQYIAPPCFTGRDTVVIQCARATQITCDTGIYI
                     FNIGCEESISTVYSFEVDCLDSIYVNNLSGWSAPAILDSAQFGRSYIVLEPTDGAGVF
                     YQPQPGYEGLDFVKVTRSFAPNQDTLLYLFRVYCGLVSKTTTSDAIQPLAWPNPVTDY
                     LFLKDVDMAPTSLRLWNLQGQAFNAHWNNLRNALQTAMLPPGIYFLTGYTTDSIPVQV
                     RFVKM"
     gene            1151..>1777
                     /locus_tag="PK198_20290"
     CDS             1151..>1777
                     /locus_tag="PK198_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF41148.1"
                     /translation="MQLKQLLPILSWLPGYDKSNLRGDLSAGLTVGVMLIPQGMAYAM
                     IAGMPPIYGLYAATIPIILYSMLGTSRQLAVGPVAMISLLTAAGVGAMAEAGSENYIS
                     LVITLALMVGVIQLLLGVFRLGFMVNFLSHPVISGFTSAAAIVIGLSQLKHLLGINMP
                     KGEHLHEILHGAWQHIGETKLLTLGIGVGAIVLIQVVKKWKKSFPGSLL"
     assembly_gap    1778..1803
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1804..>2225
                     /locus_tag="PK198_20295"
     CDS             <1804..>2225
                     /locus_tag="PK198_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF41149.1"
                     /translation="LLVWGLGLHEQGVKIVGKVPGGLPALGLPRLDWATIQSLLPIAM
                     AISLVAFMESIAVAKAMQSRHRNYKLDANQELIALGAANIGGAFLQSFPVTGGFSRTA
                     VNDQAGARTGLAGIFSAALVIVTLLFLTPLFEFLPNAV"
     assembly_gap    2226..2304
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2305..2876
                     /locus_tag="PK198_20300"
     CDS             <2305..2876
                     /locus_tag="PK198_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRF41150.1"
                     /translation="RSDFTLWLITFVATLFIGIEQGIGLGVILSLALVIFRTTRPHIA
                     VLGRVPDSDFYRNVERFSNLIQRPDILIVRFDAQLYFANVNHFKDSIDNLVSQKGEAL
                     RAIVLNAESINVHALEEVIEQYRRMNIAVYFVSVKGPVRDAMAKAHLTDKIGGGNFFM
                     SNQQAVDAFDQKNTGNTEAFADYALQTNV"
     gene            complement(2887..3816)
                     /locus_tag="PK198_20305"
     CDS             complement(2887..3816)
                     /locus_tag="PK198_20305"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF41151.1"
                     /translation="MDTNTTAAAQKIRVDFRVGRGWAWLVYTLIGILLYQLFTLQRPG
                     FGEFRLLLEGRIFEWLRSLLLYYFVFELLSVFIFLRLTLLYFSLGRLDEVALSFKGWL
                     LHQLRFLPLVLTAILVFGPITNGLRYLVVFYPDYVWGDYFPEYFFTARMYVNYLLPYL
                     VFGYLILNFNLFLNYNEWNKERFSRLSEALQTPEAPKYLQYVQAGDDEGTLLLPVDQV
                     WWFEVEGKNYMAVTNGKTYGIGLTISELEEQLDPELFFRVNRAVLVQLRFVKNYSYWE
                     NDKYILRMNDDKTEFVMQRTRLKTLKERLNPGK"
     gene            4025..5443
                     /locus_tag="PK198_20310"
     CDS             4025..5443
                     /locus_tag="PK198_20310"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF41152.1"
                     /translation="MTLSKFLFFALAAVFVAACFPSMAQSAAQSVNGAWQVTLRHRSF
                     GEMRLHLDWKVESDSTFQALSHRKGVRNLVGGFKATAGKIFKSKSNAMLRTGALTRLE
                     NGRYRMEGADMVFTGVLHTPMSSMDIKGRISGDSIRAGVFQAGTDRVLGSVDGFRTTT
                     SYRIDDYVAITHSLMEVFGENIYDARALQSKEWKEFEAKMRRFSNKAQDDLELIAAFH
                     NYSKDLKFSHKGFYRVESIKGRMRLPFAPRPNQITLEEQTPETVVLRVKSFSCTASEM
                     DSAMQIVLQKGYKNLIVDLRGNGGGSLEGGITLAGYLTIEETPTGVYLTQRWFRENPA
                     PPTAQQLETMPAFTKADVNSFAQELDDQGFVVLKARPGKQRFDGQLFVLTDRGSASAC
                     EPLTWNLKHTGRATVVGEPTAGQMLSADSYPVRSGFVAIIPNADYYTPTGERLDLVGV
                     KPNIEVKSAQALQYVLDQLAKK"
BASE COUNT         1306 a         1421 c         1360 g         1321 t
ORIGIN      
        1 ttcgttagcc tcaagctact ggctgcaagc ctcaagcgaa acccggaacc ttgaacccac
       61 cttcccacgc ctgtggcgtg gttcggtgga taaaacccgg aaccccaaac ccgctgggct
      121 ttcacatttt tacaaaacga acctgaacgg gtatggaatc ggtggtgtat cccgtgagga
      181 aatagatgcc gggcgggagc atagccgttt gcagtgcgtt tcggaggttg ttccaatgtg
      241 cattgaatgc ctgtccttgc aaattccaca gccggaggga tgtgggcgcc atatctacat
      301 cttttaagaa caagtaatcg gtgaccggat tgggccatgc gagcggttgg attgcatccg
      361 aagtcgtcgt tttagaaaca agcccgcaat acaccctgaa aagatagagc agggtgtcct
      421 gattgggggc aaaagatctc gttactttta caaaatccaa accctcataa cccggctgag
      481 gctgataaaa tacccctgcg ccgtcggttg gctccagcac gatataagag cggccaaact
      541 gcgctgaatc caggatggcc ggcgcggacc agccgctgag attgttgacg taaatggaat
      601 ccaagcagtc cacctcaaaa gaatatacgg tggaaatgct ttcttcgcaa ccgatattga
      661 aaatataaat acctgtatca caggtgattt gggtggctct ggcgcattgt atgacgacgg
      721 tgtcacggcc tgtgaaacaa ggcggcgcaa tgtattgcaa gatattggcc tcgataaaat
      781 cgcccaataa agaggagtgg ccgtaagagg cgcgcttcac aatttgcggc gggttccagg
      841 taggataccc caaactaccg atgaccgaag tgtctccgca tggcaggttg atttcttccg
      901 taaatgtttg tgcattcaac gccaacggca tcaggcccaa aaggctgata agcatccatg
      961 ttttcataat ctttgttttt tttaaagaca ggtgcgcggg caagattcgt tggcaaattt
     1021 gaagctattt gattgtatat caataagtat agtacagtac ccgctgaaat gtgaccaaca
     1081 tcacgataga ttgcctgtgc tgcgggatac ctttgccccg attttattga gaacaacaat
     1141 tggcgcatcc atgcaactca aacaacttct gcccattctt tcctggcttc ccggatatga
     1201 taaaagcaat ctgcgcggcg atctcagcgc cggcctcacg gtaggcgtta tgctcatccc
     1261 gcagggcatg gcttatgcaa tgattgccgg catgccgccc atttacggcc tgtacgccgc
     1321 caccatacct atcattttgt actctatgct gggcacctcg cggcaattgg ctgttggtcc
     1381 ggtagccatg atctccctgc tcaccgcagc cggcgtgggc gctatggcgg aggcaggctc
     1441 tgaaaattac atcagcttag tcattactct ggcactgatg gtgggcgtga ttcagttatt
     1501 gctgggcgtt ttccggctgg gatttatggt caattttctt tcgcacccgg tcatcagcgg
     1561 gttcacctcg gcggcggcca ttgtcatcgg gctgagccaa ctcaaacatc tgctgggcat
     1621 caacatgccg aagggcgagc atctccacga aatactgcac ggcgcctggc agcacatcgg
     1681 cgaaaccaag ctgctgacgc tcggcatcgg cgtgggtgcc attgtactta ttcaggtggt
     1741 caaaaaatgg aaaaaatcct tccccggttc tttgctcnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnncctcttg gtgtgggggc tgggcctgca cgaacaaggt gtaaaaatag taggcaaagt
     1861 acccggcggt ctgcctgcat tgggcttgcc ccggttggac tgggccacca ttcaatcgct
     1921 gttgcccatt gctatggcca tttctttggt ggcgtttatg gagagcatcg ccgtagccaa
     1981 agccatgcag tcgcggcaca ggaattacaa actcgacgcc aatcaggagt tgattgcact
     2041 gggtgcggcc aacatcggcg gggcgttttt gcaatccttc ccggttacgg gcggcttctc
     2101 gcgcacggcg gtcaacgatc aggccggagc gcgcacaggg ctggccggca ttttcagtgc
     2161 ggcattggtg attgtcacgt tgttgtttct tactcctctg ttcgagttct tgcccaatgc
     2221 cgtgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnatcgca gcgattttac tttatggctg attacgttcg
     2341 tcgccacttt gttcatcggc attgagcagg gcatcgggct gggcgttatt ttgtcgctgg
     2401 ccttggtcat tttccgcact acgcggccac acatcgcggt gttggggcgc gtacccgatt
     2461 cggattttta cagaaatgtg gagcgttttt ccaacctgat tcaacgcccc gacatcctca
     2521 tcgtccgttt tgatgcgcag ttgtattttg ccaatgtgaa ccatttcaag gattcgatag
     2581 acaatttggt gtcgcaaaaa ggcgaagccc tgcgcgccat tgtgctcaat gcagagagca
     2641 tcaatgtaca tgccctggag gaagtaatag agcaataccg ccgcatgaat attgccgtct
     2701 attttgtgag tgtgaaaggc ccggttcgcg atgcgatggc caaagcgcac ctgacagaca
     2761 aaatcggggg tggcaatttc tttatgagca atcagcaggc ggtggatgcc tttgaccaga
     2821 aaaatacggg caatacagag gcatttgcag actatgcgct gcaaacaaat gtttgattat
     2881 ttgtaactat ttgccgggat tcaggcgctc tttcagcgtt ttgagtctcg ttctttgcat
     2941 cacaaactcc gttttgtcgt cgttcatgcg caggatgtat ttgtcgtttt cccaatagga
     3001 gtaatttttt acaaaacgca attgcaccag cactgcccgg tttaccctga aaaacaattc
     3061 cgggtcgagt tgttcttcca attcgctgat cgtcagtccg ataccataag tttttccatt
     3121 tgttacagcc atatagttct tgccttccac ctcaaaccac cacacctgat ctaccggcag
     3181 caggagggtg ccctcatcgt ctccggcttg cacatattgc aggtatttgg gggcttccgg
     3241 cgtttgcagt gcctccgaca gtcgcgaaaa gcgctctttg ttccattcgt tataattgag
     3301 aaacaggttg aaattcagga tcaaataccc gaaaacgagg tatggcagca ggtaattcac
     3361 atacatcctg gcagtgaaaa aatactccgg gaaataatcg ccccacacat agtcggggta
     3421 aaacaccacc aaataccgca gcccgttggt gatagggccg aagaccaaaa tggccgtcaa
     3481 gaccaaaggc agaaaacgca actgatgcag caaccaacct ttgaacgaca aggccacttc
     3541 atccaaccgt cccagtgaaa aatacaatag tgtcaggcgc aaaaagatga acacagaaag
     3601 cagttcaaaa acaaaatagt acaacaacag cgagcgcaac cactcgaata tgcgcccttc
     3661 caacagcagc cgaaactcgc cgaagcccgg ccgctgcaaa gtaaaaagct ggtacaacaa
     3721 tatgccgatg agggtgtaaa ccaaccaagc ccatccccgg cccaccctga aatccactct
     3781 tatcttttgt gcagcagccg tagtgtttgt atccatatct tccatttgcg gccccgaaag
     3841 tagaaaactt tcacccgtat ttcacgccgt cctatccgta tttcaggaaa aaaagccctt
     3901 atgcgctcct tgtttcagcc ctgcaattcc ttatcccgct ccgtcgctct tgacatcggg
     3961 gaaaaggccg gtatatcttt gtcgcattca atcacattta ctttcatctg aaaaacaatt
     4021 gattatgacg ctctccaaat ttttgttctt cgctcttgct gctgtttttg tagctgcttg
     4081 cttcccttca atggctcaat ctgccgccca atctgtcaat ggcgcctggc aagtcacact
     4141 ccggcaccgc agctttggcg aaatgcgcct ccatttggat tggaaagtgg aatccgacag
     4201 cacatttcag gctttgtcgc atcgcaaagg cgtcagaaat ttagtgggag ggttcaaagc
     4261 cactgccggt aaaatcttca agtcgaaatc caacgccatg ctccgcaccg gcgctttgac
     4321 ccgattggaa aatggccgct accgcatgga gggcgccgat atggtgttta ccggcgtgtt
     4381 gcatacgccc atgtcttcga tggacatcaa aggccgtatt tcgggcgatt ctatccgggc
     4441 aggcgttttt caggcaggta ccgacagagt cttgggaagt gtggatggtt ttcgcaccac
     4501 aacgagctat cgtatagacg actatgtggc gatcacccat tcgctgatgg aggtttttgg
     4561 tgaaaacatc tatgatgccc gggcgcttca aagcaaagag tggaaggaat ttgaagccaa
     4621 aatgcgccgc ttttccaata aagcgcagga tgatctggaa ctcattgctg cttttcacaa
     4681 ctactccaaa gacctcaagt tttcccacaa aggcttctat cgggtagaaa gcatcaaagg
     4741 ccgcatgcgt ctgccgtttg cgccgcgccc caaccaaatt acgctcgaag aacaaacccc
     4801 ggaaaccgtt gtgctgcggg tcaaatcgtt ttcctgtact gcctctgaaa tggacagcgc
     4861 catgcagatc gtactccaaa aaggctataa aaatctgatc gtggacttga ggggcaacgg
     4921 cggcggctcg ctcgaaggag gcattacgct ggccggctat cttactattg aggaaactcc
     4981 caccggcgta tatctcacgc agcgttggtt ccgcgaaaac cctgcgccgc ccaccgcaca
     5041 gcagttggaa accatgcctg catttaccaa agcagatgtg aattcttttg cacaagagtt
     5101 ggacgatcaa ggttttgtgg tgctcaaagc ccgccccggc aagcagcgct tcgacggcca
     5161 attgttcgtg ctcacggatc gcggctctgc cagcgcctgc gaaccgctga cctggaatct
     5221 gaaacacacc ggcagagcta ccgtcgtggg cgaaccgacg gccggccaga tgttgagcgc
     5281 cgattcttac cctgtgcgca gcggatttgt ggccatcatt cccaatgcag attattacac
     5341 gcccaccggc gagcgcctcg atctcgttgg cgtaaagccc aatattgaag tgaaaagcgc
     5401 acaggcactg caatatgtgt tggatcaatt ggctaagaaa taaactccgg tcagggtcag
     5461 gcggcaactt cttgttcgct gcctggcttt ttcgctctgc ttccaagagg atc
//
LOCUS       DAORLF010000325         5500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8085_length_5500_cov_1.635078, whole genome shotgun sequence.
ACCESSION   DAORLF010000325 DAORLF010000000
VERSION     DAORLF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8085_length_5500_cov_1.635078"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    225..359
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<360..>1118)
                     /locus_tag="PK198_20315"
     CDS             complement(<360..>1118)
                     /locus_tag="PK198_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41153.1"
                     /translation="RNNYAISSAELTEWIKAIPALKQVMVIDACNSGKIVEDLAVRKD
                     LSSTQIRALDRMKDRTGMFILTGSAADMVSYEAGQYGQGLLTFSLLEGMSGPALSDGN
                     VVDVMTLFQHARNRVPELARGIGGIQSPVLAFPDGGASFDIGLVGPGVKIPLAKAKPV
                     FVRNNFLDDDIFDDRLGLSNALADYFRSITSKGAQADMLYWDNVNEYENAYSIKGLYT
                     LAGDAVTVRARLFKGNEVVGDKFSVTGKKSNVPA"
     assembly_gap    1119..1175
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1176..>1384)
                     /locus_tag="PK198_20320"
     CDS             complement(<1176..>1384)
                     /locus_tag="PK198_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF41154.1"
                     /translation="DKVAITLLNTRADDPERQGISSKAAIRQAFVDLVIKAKAQDVLM
                     VYFSGHGVTYGAAENAQFYYLTKDI"
     assembly_gap    1385..1529
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1530..>3058)
                     /locus_tag="PK198_20325"
     CDS             complement(<1530..>3058)
                     /locus_tag="PK198_20325"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF41155.1"
                     /translation="RDSLSFSGHAVIISSIAFSPDGQKLLTGSFDKTAGMWSLSGQEM
                     QRFRGHSFPISSVAWSPQGRQILAGSTDGMAKLWHLPGGDVQTFRGHDTLVWSVAFSP
                     DGQQVLTASADSTAKLWNLSGRETQTFKGHRSTVYSAAFSPDGRQVLTASADSTAKLW
                     NLSGREIQTFKGHASFVRSAAFSPDGRQVLTGSGDRSAKLWDLSGGVKHTFSDPAGRV
                     SSVAFSPDGQKVLTGGSDKTAKLWAASGGDPRTFSGHADALTSVAFSSDGQKILTGSG
                     DRTAKWWSLSSPDTLTLRGHTSFVRSAVFSPDGRKVLTGSDDNTVKIWDANSGQCLMT
                     FISLDTSEWVVTTPFGLFDASPGAMKLMYYTQGLEVIELGQLKERYYEPGLLGKLLGF
                     NDDPLRSVEGFGALPLYPKLDAAIGADQLSLAVTLTPRSGGIGKLSLFVNGKEVLEDA
                     NPQRAERVSIDLTQFAEYYLPGENNLLALRAYNAQGWLKSSALELGYQPPAIARGTSS
                     GP"
     assembly_gap    3059..3233
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3234..3842)
                     /locus_tag="PK198_20330"
     CDS             complement(<3234..3842)
                     /locus_tag="PK198_20330"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF41156.1"
                     /translation="MKKTPFILMFLALAAGLAAQVPQLVVPIGHTGIVTAAAFSPDGQ
                     QVLTGSWDGTAKLWDLRGREIKTFSGHTSGVAFSANGQQVLTASFDNTVKLWSLSGGE
                     PLTFKHTSHVNSAAFSPDGKTVLTGSGDGIARLWNVSGGAPKTLSGHDSEITSVAFSP
                     DGQKVLTGSFDKTAKLWDLSGGAPQTFSGHFSVVSSVAFSPDG"
     gene            4776..4958
                     /locus_tag="PK198_20335"
     CDS             4776..4958
                     /locus_tag="PK198_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41157.1"
                     /translation="MNTLEERRQKEEERRQKEQALEQAREARIKLARLLLRTGMSAKD
                     AALEAGLSEEELKKME"
     gene            complement(4968..>5500)
                     /locus_tag="PK198_20340"
     CDS             complement(4968..>5500)
                     /locus_tag="PK198_20340"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF41158.1"
                     /translation="FSKTVSRYASGQQKLRLCGYSMGGYVSLYFASLFPEMVEKVFTL
                     GTKLAWTPETGAKEAGRLDPTKILEKVPLFAASLAQKHGADHWEKNVANTAGLLRALG
                     EAPPLTNEALGRIQAEVLMARGSLDNMVSEEECRQAVGLIPRASYVRLEDQPHPLDQC
                     DPSVVAAQLRAFFNEG"
BASE COUNT         1079 a         1454 c         1321 g         1134 t
ORIGIN      
        1 ggtttgccaa agcccatcac cacatcgttt tcagcggttt tataaatacc aatggtgaga
       61 atgccggaaa gcgcctgctc caacggcgta atgtcttcgc tctggccata agccgcccaa
      121 ctgaaagcga gcagaagtaa taatgcaacc atgcgtgtca tagtatgtag atttgaggtg
      181 gtaaaatacg ataatcaatg atttatcgct gtaaaattca tttgnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
      361 aggccggcac attgctcttc ttgcccgtta ccgaaaattt atctcccacc acctcattgc
      421 ctttgaacag ccgtgcgcgc actgtcaccg catcgccggc gagggtgtat agacctttga
      481 tggaataggc attttcgtac tcgttcacgt tgtcccagta cagcatatcg gcctgcgcgc
      541 ctttggatgt gatgcttcgg aagtaatcgg ccagtgcatt gctcaggccc agtctgtcgt
      601 cgaagatgtc gtcgtccaaa aagttgtttc tcacaaatac cggcttggct ttcgccaaag
      661 gaatcttcac tcccggcccc acaaggccga tgtcgaaact ggccccgccg tcggggaaag
      721 ccagcacggg gctttgtatg ccgccgatac cgcgggccaa ttcgggcacc cggttgcggg
      781 cgtgctgaaa cagcgtcatc acatccacta cgttgccgtc actcaaagcc ggaccgctca
      841 tgccttccaa caggctgaac gtgagcaaac cctgcccgta ctgccccgcc tcgtaactca
      901 ccatatcggc agccgaaccg gtaaggatga acatgcccgt gcggtctttc atgcgatcca
      961 atgcccggat ttgggtggaa ctgaggtctt ttcgcaccgc caggtcttct acgatcttgc
     1021 ccgaattgca ggcgtctatc accatcacct gcttgagcgc agggatggct ttgatccact
     1081 ctgtcagttc agcggaggaa atggcgtagt tgtttctgnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctatg tctttggtca gataataaaa
     1201 ttgagcgttt tcggcagcgc cataggtcac gccatgccct gaaaaataga ccatcagcac
     1261 gtcctgagct ttggctttga ttaccaaatc tacaaatgcc tggcgtatgg ctgccttgct
     1321 cgaaatgccc tgccgctccg ggtcatcggc gcgggtgttg agcagcgtga tggccacctt
     1381 gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggccggagga ggttccgcgc gcgatggccg
     1561 ggggctggta gccgagttcc aatgccgagc ttttcagcca gccctgcgcg ttgtatgccc
     1621 gcaatgccag caggttgttt tcgccgggca ggtagtattc agcgaattgc gtcagatcaa
     1681 tgcttacacg ctcggcgcgc tgtggattgg cgtcttccag cacttctttg ccgttgacaa
     1741 agaggctgag ctttccgatg ccgccggagc ggggcgtcag cgttacggcg aggctgagtt
     1801 ggtcggcgcc gatggcggca tccaatttgg ggtacagcgg caaagcgccg aagccctcca
     1861 ccgagcgcag cgggtcatcg ttgaacccca gcagcttacc cagcaggccg ggttcgtagt
     1921 agcgctcttt gagttgtcct aattcgataa cttccagacc ttgagtgtaa tacatcagtt
     1981 tcatggcgcc gggcgaagca tcgaacaggc caaatggcgt agtgacgacc cactcgctgg
     2041 tatcgagcga aatgaaggtc atcagacatt ggccgctatt tgcatcccaa attttcacag
     2101 tattgtcgtc acttcccgtc aacaccttcc ggccatcggg cgaaaagact gccgaccgaa
     2161 cgaaggaggt atggcctctc aatgtcaggg tgtcagggga ggataaactc caccattttg
     2221 ctgtgcggtc tccgcttccg gtcagaattt tttggccatc cgatgaaaag gcgaccgagg
     2281 tgagcgcgtc ggcgtgccct gaaaaagtgc gcggatcgcc gcccgacgca gcccataact
     2341 tcgctgtttt atcacttcca ccggtcagca ctttttgtcc atccggtgaa aaagcgaccg
     2401 acgaaactct gccggccggg tccgaaaatg tatgctttac gccgcccgac aagtcccaca
     2461 actttgcgct tcggtccccg cttcccgtca aaacttgccg gccatcgggc gaaaaggctg
     2521 ccgaccgaac gaaggaggca tggcctttga atgtttgaat ttcccgcccc gacaggttcc
     2581 aaagtttggc cgtactgtct gcgcttgccg tcaacacttg ccgaccgtcg ggcgaaaagg
     2641 cagccgaata aacggtcgaa cggtggcctt tgaaggtttg agtttccctt cccgacaggt
     2701 tccacaactt cgccgtgcta tctgcgcttg ccgtcaacac ctgttggccg tcgggcgaaa
     2761 acgctaccga ccagaccaaa gtatcatgcc cccgaaatgt ttgaacatcg ccccccggca
     2821 agtgccacag cttcgccatc ccgtctgtgc ttcccgccag tatctgccgc ccttgaggag
     2881 accaggccac agaactgatg ggaaaggaat gcccccgaaa acgctgcatt tcctgccccg
     2941 acaggctcca cattccggcc gttttgtcaa aacttcccgt cagcagtttc tgaccatcgg
     3001 gcgaaaaggc gattgaactg atgatgacgg catggcccga aaaggagaga gagtctctnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtcg
     3241 ggtgaaaagg ccacagaact gacgacggag aaatgcccgc tgaacgtctg cggggcgccg
     3301 cccgacaggt cccacaactt tgcagtctta tcaaagctcc ccgtcaacac tttttggccg
     3361 tcgggcgaaa acgcaacaga ggttatctcg gaatcgtggc ccgacaaggt tttaggcgcg
     3421 ccgcccgata cattccaaag tctggcaatt ccgtcgccgc ttccggtcag caccgtttta
     3481 ccgtccgggg aaaaggctgc ggaattgacg tgggaagtat gcttgaaggt cagcggctcg
     3541 ccgcccgaca agctccacaa cttcacagtg ttgtcaaagc tggccgtcag cacctgttgg
     3601 ccattggccg aaaaagctac cccagaagta tgcccgctga aggttttaat ttcccgcccc
     3661 cgcaagtccc acaatttcgc cgttccatcc caactgccgg ttaagacctg ttggccgtcg
     3721 ggagaaaaag ccgccgccgt tacaatgccg gtatgcccga tgggcacgac caattgcggg
     3781 acctgtgctg ccagcccggc tgccaatgcc aaaaacatga gtatgaaagg tgtttttttc
     3841 atgacttgta ttttgaattt tccatgctta gtgaaggggt gactacggag ttgcaccctt
     3901 gattaagtca ccccttcact cgcctatact acttcaccat cccttgtacc ttccccacca
     3961 atgactccac cagcgcaggc agggcattct tattgcccgt caccgtaaac gctgagccca
     4021 ccaccgtttt gcctttgaac aagcaggcgc gcaccgtcac cgcatcgccg gcgagggtgt
     4081 agcgcccttt gatcgagtag gcattttcgt gctagcttgc attccagtac agccggcagc
     4141 aacagaaaaa caaggcgttt tctcatgtct gatatggatt ttggctaaaa acgaatactg
     4201 ataattgtgg cgggtgcccg ctctacctgc ctcccccggc ccctccaaag gaggggagtc
     4261 gtcaggctgc gggcaccgcc tgcctgccgg taggcatggg gtcatccgct acatggcctc
     4321 ctaaggtcgg ccatccgctc cggcccctgc cgcaacgcga tccatccggg cggcgctttt
     4381 ctactacctt ccatttgcct gaaccgcaaa aagtgacctc tccggctggt aaatgtacct
     4441 gttttccggc tttgtaacgg cagcgcagcc tgccttgtga tgtgcagaca gcgtgccgca
     4501 tggtgcgaag atacgcacgg cgcggtggat ttagtgctaa catgaagtgt tttccctagg
     4561 ggttgtgtaa agagtccctt gctgggcggt gcccttaagc gggagcgatt cccccccccc
     4621 aaatcatgcg cattttctct acagccctct cttggagttt gtgacgaaga ttttaggccc
     4681 caaaggtaaa aaaagaggtt tttaggctcc ttttttggcc ttttgatgct tctgtgccat
     4741 ttttatcgca agttactttt ttgtgacaca cttttttgaa tactctcgaa gaacgcaggc
     4801 agaaggaaga agaacgcagg cagaaggaac aagcacttga gcaagcgcga gaggcccgaa
     4861 ttaaattagc tcgcctgctg ctgcgcacgg gcatgtccgc caaagatgcg gcgcttgaag
     4921 ccgggctgtc ggaggaggaa ctaaaaaaaa tggaataatc cagcccctca gccctcgtta
     4981 aaaaacgccc gcaactgagc cgccactaca ctggggtcgc attgatccaa agggtgcggc
     5041 tgatcctcca agcgcacata gcttgcccgc gggatcagtc ccacagcctg ccggcattct
     5101 tcctccgata ccatattgtc caaacttccc ctcgccatga gcacctccgc ctgtatgcgc
     5161 cccaaggctt cattggtcag aggcggcgct tcgccgaggg cgcgcagcag cccggctgta
     5221 ttggctacat ttttttccca atgatctgcg ccgtgttttt gggccagaga cgcagcaaac
     5281 agcgggactt tttccaaaat tttggtcgga tccagtctgc ctgcctcttt ggcgccggtt
     5341 tcaggcgtcc aggccaattt ggtgcccagg gtaaacactt tttccaccat ttcaggaaaa
     5401 agggaagcga aatagagcga cacatacccg cccatgctgt atccgcagag gcggagtttt
     5461 tgctggccgg aagcgtagcg gctcaccgtt ttggaaaatt
//
LOCUS       DAORLF010000326         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8160_length_5461_cov_2.107473, whole genome shotgun sequence.
ACCESSION   DAORLF010000326 DAORLF010000000
VERSION     DAORLF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8160_length_5461_cov_2.107473"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..864
                     /locus_tag="PK198_20345"
     CDS             208..864
                     /locus_tag="PK198_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41159.1"
                     /translation="MKLSPSAVLLPALMLFATVFLSAQKPSKLPLAKYYDAAGKELSK
                     AEFDQTERTQNYLSVIVDSSANAHQLVWREEWGTLPSAELIYSKIEAAIGKPVDRNKY
                     LLIMYYPGKDPCNSSGMATPSDYAHNDAELTRHIQKIAPTQVIHIYKSPTGLKKLYGS
                     LSKHHQDPEALIERTFFKYHYPCGSYVIIRPDGAYGSYFGEYAYEDILERAKALKKKE
                     "
     gene            complement(882..1265)
                     /locus_tag="PK198_20350"
     CDS             complement(882..1265)
                     /locus_tag="PK198_20350"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF41160.1"
                     /translation="MFVGDGGFQMTLQELGLCAQWNVAVKIVLLDNEFLGMVRQWQQL
                     FYERRYSSVDLVNPDFIKIAEGFGVPGRRIDQPDQMEAAIAEMLGHDGPYLLHVRVEK
                     EENVFPMVPSGKAVDEIVLDQSGLG"
     gene            complement(1235..2659)
                     /gene="ilvB"
                     /locus_tag="PK198_20355"
     CDS             complement(1235..2659)
                     /gene="ilvB"
                     /locus_tag="PK198_20355"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRF41161.1"
                     /translation="MTASKVYINEVVGSGATQPETSNKSGMAGKTVSGAEAVLLCLLE
                     EGVHTMFGYPGGAIMPVYDALHYYEDRIRHILPRHEQGAIHAAEGYARVTRQTGVCMA
                     TSGPGATNLVTGISDAMLDSTPLVCITGQVGKNLLGSDAFQETDVIGCTTPITKWNYQ
                     ITSAHEIPEVFAKAFYIANSGRPGPVVIDITKNAQMEELEFHYHPRPVIRSYHPVPKP
                     TQEAIQAAADLINAAEKPMILAGHGIQIAHAQEVFKAFVEKTGIPLGCTIHGLSSIPS
                     DHPLHTGMLGMHGNYGPNLKQNECDVLIAIGMRFDDRVTGRLDGYARQAKVIHIEIDP
                     SEINKNVKAHVALPGDAKVTLEALLPLVQPKSYPDWVESFRACDRIEHDRVILSATSP
                     TPSGGLTMGMVVKEVSRQTEGMAIVATDVGQHQMISARYYEFKDTNQWVSSGGAGTMG
                     YGLPAAFGAPISRWSCLLATAAFR"
     gene            complement(2736..>2942)
                     /locus_tag="PK198_20360"
     CDS             complement(2736..>2942)
                     /locus_tag="PK198_20360"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRF41162.1"
                     /translation="GGLIGLLQDGDHIRIDSVENKIEALLSEEEINQRKAQWTPRSLP
                     VQSGYLAKYAKMVASASQGCVTDM"
     assembly_gap    2943..2970
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2971..4420)
                     /gene="ilvD"
                     /locus_tag="PK198_20365"
     CDS             complement(<2971..4420)
                     /gene="ilvD"
                     /locus_tag="PK198_20365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRF41163.1"
                     /translation="MEQWNRHSRTLTEDESLPAAQAMLHGIGLTEADFRKPQVGIVST
                     GWEGNPCNMHLNGIAARLKQEVQTRDMVGLIFHTIGVSDGISMGTDGMRYSLPSREII
                     ADSIETVAGAQWYDAVAAIVGCDKNMPGAMMALARLNRPSLLVYGGTIRPGFYGDKKL
                     DIVSAFEAYGRKTAGSIPEEEYREIIRRAIPGPGACGGMYTANTMASAIEALGMSLPM
                     NSSLPAASHEKDAEPARVAAALRHLLEINLRPSDIMTPQAFDNAMTVLTVLGGSTNAV
                     LHLIAMAKAAGVPLSIDDFQRISNKTPFLADLKPSGKYVMEDLFLVGGVPAVMRMLLD
                     NGFLHGDCMTVTGKTLAENLADAPQLSPGQDVVRPLSEPIKATGHIQILYGNLAEEGA
                     VAKITGKEGEVFSGPARVFDGEAAANQAIADGAIRPGDVIVIRYCGPKGGPGMPEMLK
                     PTAAIIGAGLGDSVALITDGRFSGGTHGFVVGH"
     gene            5044..>5461
                     /locus_tag="PK198_20370"
     CDS             5044..>5461
                     /locus_tag="PK198_20370"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF41164.1"
                     /translation="MAEWINSAFRVYLRQRYRQIERFMQQPHETQRLLWRQLMQYAQH
                     TEWGKRYDYGSLRSPEDYARRVPVQDYESAKPHIQRMMHGERDVLWPGRVKWFSKSSG
                     TTSDKSKFIPVPGVKLRQCNLMGSRDAMALFYHRRPD"
BASE COUNT         1121 a         1497 c         1601 g         1214 t
ORIGIN      
        1 catttctggt gccaacgaac ttttctttgc cgcccgcatc aacaaattcg acaacatggg
       61 cgagtttgag gtagctgcac ccgtagaaga aaaacccaag aagcgaaagt aagcggcgtt
      121 tacaagacat gccgacctgc ttgtgcaggt cggcatgtcg tctcaatagt tatagacaat
      181 actctccatc atcaaaaact accatccatg aaactttctc cctctgccgt cttgcttccg
      241 gccttgatgc tgtttgctac cgttttcctg tcggctcaaa agccatccaa actgcccctt
      301 gccaagtact acgatgcggc cggcaaagag ctttccaaag cagaattcga tcagacagag
      361 cgcactcaga attatctgtc ggtcatcgtt gattcctcgg ccaatgccca tcagctcgtc
      421 tggagggaag agtggggcac actgccttca gcggaattga tctattcaaa gatagaagcc
      481 gccatcggga aacccgttga ccggaataag tacctgctga tcatgtacta tccaggcaaa
      541 gacccttgta actcaagcgg catggctacc ccatctgact atgcgcacaa tgacgctgaa
      601 ttgacgaggc atatacaaaa aattgctccc actcaagtca ttcacattta taaaagcccc
      661 acaggcttga agaagctgta cggaagttta tcaaagcatc accaagaccc cgaggccctc
      721 atagagcgca cttttttcaa ataccactat ccctgcggca gctatgtcat cattcggccc
      781 gacggcgctt atggcagcta cttcggagaa tacgcgtatg aagatatttt ggagcgggct
      841 aaggcgctga aaaagaagga gtaagcccgg ctttttaccc atcaccccag ccccgactgg
      901 tccaacacaa tctcgtccac agctttgccg ctgggtacca tcgggaacac attttcctct
      961 ttttctaccc ggacgtggag caggtaaggg ccgtcgtggc cgagcatttc agcaatggcc
     1021 gcctccatct ggtcgggctg gtcaatgcgg cgccccggta cgccgaagcc ctctgcaatt
     1081 ttgataaaat cgggattcac caagtccacc gacgagtagc ggcgctcata aaagagttgc
     1141 tgccactggc gcaccatgcc gagaaattca ttgtctaaca acacgatctt gaccgccaca
     1201 ttccactgcg cgcacaggcc cagttcctgc aaagtcatct gaaagccgcc gtcgccaaca
     1261 aacaagacca cctgcttatc ggggctccga aagccgccgg cagtccatac cccatcgtgc
     1321 ccgcaccccc cgaagacacc cactggttgg tgtctttgaa ttcgtaatac cgggcggaga
     1381 tcatctgatg ctggcccaca tcggtagcca cgatggccat gccttctgtc tggcgggaca
     1441 cctccttcac caccatgccc atggtgaggc cgccggaggg cgtggggctt gtcgcgctca
     1501 gaatgacgcg gtcgtgttct atgcggtcgc aggcgcggaa gctctctacc caatcgggat
     1561 aagatttcgg ttgcaccaaa ggcaacagag cttccagcgt gactttggcg tcgccgggca
     1621 gcgccacatg cgccttcacg tttttgttga tttcggaagg atcaatctct atgtgaataa
     1681 ctttggcctg gcgggcgtac ccgtccaatc gcccggtcac gcgatcgtcg aagcgcatac
     1741 caatggcgat gagtacgtcg cattcgtttt gtttgaggtt ggggccgtaa ttgccgtgca
     1801 tgcccagcat cccggtgtgc agcgggtggt cggacgggat ggacgaaagg ccgtgtatgg
     1861 tgcagcccag cggaatgccc gttttttcta caaatgcctt aaaaacctcc tgtgcgtgcg
     1921 caatttgtat gccgtgcccg gccaggatca tcggtttttc ggcggcgttg atgagatcgg
     1981 cggcggcctg aatcgcctcc tgcgtgggct tgggcacggg gtggtaactg cgaatgacgg
     2041 gccgggggtg gtaatgaaat tccaactcct ccatctgggc atttttggtg atgtctatga
     2101 ctaccgggcc tggcctgccg gaattggcaa tgtaaaaagc cttggcaaac acctccggta
     2161 tttcgtgtgc cgaggtgatc tgatagttcc acttggtgat gggcgtagta cagccgatca
     2221 catccgtttc ctgaaacgca tccgagccga gcaggttttt tcccacctgg ccggtaatgc
     2281 aaacgagcgg cgtagaatcg agcatggcgt cggaaatacc ggtaaccaaa ttggtggcgc
     2341 ccggccctga tgtagccatg cacacgccag tctggcgggt cacgcgggca tacccctccg
     2401 cagcgtggat ggcgccctgt tcgtggcgcg gcaggatgtg ccggatgcgg tcttcgtaat
     2461 aatgcagggc atcataaacg ggcatgatgg cgccgcccgg atagccgaac atggtatgaa
     2521 cgccttcttc caacaaacaa agcaggacgg cctctgcgcc gctcacggtt ttgccggcca
     2581 tgccggattt gttgctggtt tcgggctgcg ttgcgccgct gccgacgacc tcgttgatgt
     2641 acaccttgga tgctgtcatt ttgtggtgtg ttttttttct aaatggataa gatgatgcgt
     2701 tgcgcgttca ggaggagacg ttgcatgaaa cgtctctaca tatccgttac gcaaccttgg
     2761 ctggcggagg ctaccatttt ggcgtatttg gccaagtagc ccgactgcac gggcagggaa
     2821 cgcggcgtcc attgcgcttt tctttggttg atttcctcct cgctgagcag cgcctcaatc
     2881 ttattttcca cgctgtctat gcgaatgtgg tcgccgtcct gaagcaagcc tatgaggccg
     2941 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtggcccac tacaaagccg tgcgtgccgc
     3001 cggaaaagcg cccgtcggtg atgagcgcca cgctgtcgcc cagtcctgcg ccgatgatgg
     3061 cagcagtagg tttgagcatt tcgggcatgc cggggcctcc tttggggccg cagtaacgaa
     3121 tgacgatcac atcgccgggg cgaatggcgc cgtcggcaat ggcctgattg gccgccgctt
     3181 cgccgtcgaa aacgcgcgcc gggccgctga agacctcgcc ctctttgccg gtaattttgg
     3241 ccaccgcgcc ctcttcggcc agattgccgt acaaaatctg aatatggccg gtagctttga
     3301 tcggctcact taaaggacgc accacgtcct gtccgggact gagttgcggc gcatccgcga
     3361 gattttcggc gagggttttg ccggtcacgg tcatacaatc gccgtgcagg aagccattgt
     3421 cgagcaacat gcgcatgacc gccggcacgc cgcccacgag gaagaggtct tccatgacgt
     3481 attttccgct gggcttgagg tcggctaaga aaggcgtttt gttgctgatg cgctggaagt
     3541 cgtctatgct cagcggtacg ccggctgctt tggccatggc gatgaggtgc aaaacggcat
     3601 tggtggagcc gcccagcacg gtgagtaccg tcatggcatt gtcgaaggcc tgcggcgtca
     3661 tgatgtcgct gggtcggagg ttgatttcca acagatggcg cagcgctgcc gccacgcggg
     3721 cgggttcggc gtctttttcg tgggaggctg ccggcaagga ggagttcatg ggcaggctca
     3781 tgcccagcgc ttcgatggcg gaggccatgg tattggcggt gtacatgccg ccacaggcgc
     3841 cggggccggg aatggcgcgg cgaatgattt cgcggtattc ttcttcgggg atgctgccgg
     3901 cggttttgcg gccataggct tcgaaggcag aaacgatgtc caatttttta tcgccgtaaa
     3961 agccgggccg gatggtgccc ccatacacta agagcgaggg ccggttgagg cgggccagtg
     4021 ccatcatcgc gccgggcatg tttttgtcgc agcccacgat ggcggccacc gcgtcgtacc
     4081 actgggcgcc ggccacagtt tcgatggaat cggcgatgat ttcgcgggag ggcagagagt
     4141 agcgcatgcc gtcggtgccc atagagatgc cgtcgctgac gccgatggtg tgaaaaatca
     4201 gcccgaccat atcccgggtc tgcacttcct gtttgagccg ggcggcgatg ccgttgaggt
     4261 gcatattgca ggggttgcct tcccaacccg tgctgacgat gccgacctgg ggcttgcgaa
     4321 aatcggcttc ggtgaggcca atgccgtgca acatggcttg tgcggcgggc aggctttcgt
     4381 cttcggtgag ggtgcggcta tggcgattcc attgttccat gatgcgtgtg tttttgacaa
     4441 ttttatatac aacaagagcc gcctcacttt aagaagaacg gctcttgctg cggatgcgac
     4501 ccttgtcgga gcctattctc cctctctgtg tcgaatgatt tcgacgacta cattggaaat
     4561 gtgcgaacgg agaacagaca tgacggcgct ggtttttcga tttgttgata agcggagcca
     4621 aagttggcgc gcattttcag agggtgcaag tttttgggcc tttttttgaa aatgagatag
     4681 agatcgccaa agattattgt ctttttggca ataaaaagag gctggagggc aatttttggt
     4741 agctgttggg tgcttggcag tgcgccaggg gtagtagcgg tatgccgcag agggcataga
     4801 gcatagcggg cagacaagcg aagcgcagtc ccggatagcc tgggggagag acccaatgcg
     4861 gcatgagagt ggatgacccc tgcctgccgg caggcacggc gacccgccgc cctgtctgct
     4921 accctccttt ggaggggctg ggggtggccc cctgtggcgg gtggcgccat aatggttcgg
     4981 aaaacccatg tctgcaccat ctcccatttt atttctaact tcgcctgagc tttccgtacg
     5041 atcatggcag aatggatcaa cagtgctttc agggtatatc tgcgccaaag gtaccggcag
     5101 attgaacgct tcatgcagca gccgcacgaa acacagcgcc tgttgtggcg gcaactcatg
     5161 cagtacgcgc agcacaccga gtgggggaag cgctatgact acggcagcct gcgcagcccg
     5221 gaggactatg cccgacgggt gcctgtgcaa gactacgaaa gtgcgaagcc ccatatacag
     5281 cgcatgatgc acggcgaacg cgacgtgttg tggcccggcc gggtgaagtg gttttccaaa
     5341 tcgtcgggta ctacgagcga caaaagtaag ttcataccgg tgccgggcgt gaaactgcgg
     5401 caatgcaacc tgatgggcag ccgcgatgcg atggccttgt tttaccaccg gcggcccgat
     5461 g
//
LOCUS       DAORLF010000327         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8161_length_5461_cov_1.896226, whole genome shotgun sequence.
ACCESSION   DAORLF010000327 DAORLF010000000
VERSION     DAORLF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8161_length_5461_cov_1.896226"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..>654)
                     /locus_tag="PK198_20375"
     CDS             complement(269..>654)
                     /locus_tag="PK198_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRF41165.1"
                     /translation="GTLTTLSAGLRYRRQPWGNFNVDVEYNRLQLPDPYGQSTLLLIR
                     PRIEINFSRSIFWTTFLQLNTQRENFNINSRLQWRFAPMSDLFVVYTDNYLAESETID
                     GRFRFTSFAPRNRALVLKLSYWLNL"
     assembly_gap    655..664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<665..2498)
                     /locus_tag="PK198_20380"
     CDS             complement(<665..2498)
                     /locus_tag="PK198_20380"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF41166.1"
                     /translation="MKNTLLFTALFVLAFHALAQNAAIYQEQYRVQILRTAETITLDG
                     NLTEPVWQSAEKAADFWQKWPRDDVKAHLKTEVQLAWNDDYLYLAATLYDTGRHVIQT
                     MKRDVDFWDRDGFAFVLDPVNEKTYGFLFGVSAAGVQTEGLLQAFDEPGMEWDNRWFS
                     AVQQHADRWTLEMAIPFKTLRYEEGKTVWGVNFIRNDIKNNQYHTWTQVPQQYPGVDF
                     GYMGALVWDQSPQKSKGNIALIPYLTGGTNVDFENKTPASFTGGLGADAKVALSAALN
                     LDLTVNPDFSQIEVDQQVTNLTRFNIFFPERRNFFLENSDLFAGFGVPPIRPFFSRTI
                     GLDKNVQPVPILFGARLSGNLDKNWRIGLMDMQTGGKNGEPAQNYFAAAFQRKLFARS
                     NIKAMFLNRQDIGTENNDYRRNADLEFTYQNAAGNWTGWSGYHKSFQPGSKGQDAFFN
                     AGIWHNGRRFSTIVDVLSMGSNYRADMGFIERIENYDAERDSVIRLGFTYIFNESNYV
                     IFPRNSKLINTHSFGIETFLVYNPDDSFNERFNRWRYFIEFKKRSELQFRVDNNLVAL
                     QFPFSFTDGAPLPRSRYDNYRLNVEYESDGRKTFSWEIAAEHWPS"
     gene            complement(2559..3206)
                     /locus_tag="PK198_20385"
     CDS             complement(2559..3206)
                     /locus_tag="PK198_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41167.1"
                     /translation="MRPETFLQFVKKARLPVFFTWPLFLWYLLAMAMLIFGGHHSSAL
                     DIIVSVIIVSTGYGMLMYWFYRVTFTIYKAGQKAYLELDAHQIEIHENVKIYIKGFDL
                     FSQKKFFPPSIEKTNYDFDAADFILTKNSIVLLGKSSAFGGLSYTYPIEIVITKRLTN
                     MPAASFISRGLDGKGRLEMRIQDDHYSRPVTIVIKDRNHSDYLLNWLVSNSKSHQ"
     assembly_gap    3474..3564
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3565..4264)
                     /locus_tag="PK198_20390"
     CDS             complement(<3565..4264)
                     /locus_tag="PK198_20390"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HRF41168.1"
                     /translation="MSDSRPLVSFDWAIKRLLRQKANYDVLEGFLTVLLRRQITIINI
                     PESQGNKDDATDKVNAVDILCESEDKELILIELQYNSESSYFHRMLFGVSKTITDNMF
                     EGFEYDKVRKVYSINIVYFDLGQGSDYVYHGTTEFRGIHTHDVLRVNARQREVFDVTA
                     PYELFPEYYIIKVNSFDDVAKDTLDEWIYYLKNNRLPEHFTAPGLEKAEKILNYYDMN
                     AEAKALYDAHIKARR"
     gene            complement(4458..5342)
                     /locus_tag="PK198_20395"
     CDS             complement(4458..5342)
                     /locus_tag="PK198_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRF41169.1"
                     /translation="MFFQRLPIFVLLFAAALTLNAQDDLLSLLGDDDKEINFANAAFK
                     TNRVINLHSLENTAPGVLDFKISHRFGTLNGGFYEMFGLDNATIRLGLDLGITDNLAV
                     GLGRSSYEKTYDGFIKYKFLRQSTGARNMPVTAAVVATAAIQTLRWPDPDRENLFTSR
                     LYYTWQLIVGRKFNDNFSLQLSPTLVHRNLVRTRDEKNDVPALGISGRYKLNKRIALN
                     AEYIYVMPNTLAPEFKNALSIGFDIETGGHVFQLHLTNATSMIERGFVAENTNTWGNG
                     GIHFGFNISRVFTLWEPK"
BASE COUNT         1193 a         1211 c         1606 g         1350 t
ORIGIN      
        1 agctcctgat caatagggcg ttgtatgtcg ttgatgcttt tactcatgcc gccgcagtgt
       61 gttttttcgg ccccaaactt acgaataaga ccaacaatca tagggggtaa ttgttccggg
      121 gcggagttct tgcctgtgcg ccaggggcag caccacgccg ttagcgtggg gatgacccct
      181 gccttccggc aagcaggcgg ccccgccggc ttgatgcgcg aggctttgag gggcggccgg
      241 cagagtggcg gggaggcgcc ccaacccctt acaaattcag ccaataactc agtttcagca
      301 ccaaagcccg gttgcgggga gcgaaggagg tgaagcggaa tcgcccgtca atggtctcac
      361 tttcagcgag atagttgtcg gtataaacga cgaagagatc ggacatgggc gcgaaacgcc
      421 attggaggcg gctgttgatg ttgaagtttt cgcgttgggt gttgagttgc aggaaggtgg
      481 tccagaagat gctgcgcgag aagttgatct cgatgcgggg ccggatgagg aggagggtgc
      541 tttggccgta aggatcgggt agttggaggc gattgtactc tacgtccacg ttgaaattgc
      601 cccagggctg gcggcggtag cgcagcccgg cgctgagggt ggtgagcgtg ccggnnnnnn
      661 nnnnaggagg gccagtgttc ggcggcgatt tcccaggaga aagtttttcg gccgtcggat
      721 tcatactcca cattgaggcg gtagttgtcg tagcggcttc ggggcagcgg ggcgccgtcg
      781 gtgaaggaga aggggaattg cagggccacg aggttgttgt ccacccggaa ttgtagttcg
      841 ctgcgctttt tgaactcgat gaagtaccgc cagcgattga agcgctcgtt gaaactgtcg
      901 tcgggattat agaccaaaaa ggtttcgatg ccgaaggagt gggtattgat gagcttgctg
      961 ttgcgcggga agatgacgta attggactcg ttgaaaatat aagtgaatcc cagccggatg
     1021 acggaatcgc gttcggcgtc gtagttttcg atgcgttcta tgaagcccat gtcggcgcgg
     1081 taattgctgc ccatgctcag cacgtccact atggtgctga aacggcggcc attgtgccag
     1141 atgcctgcgt tgaaaaaagc gtcctgccct tttgagccgg gctgaaagga cttgtgatag
     1201 ccgctccagc cggtccagtt gccggcggcg ttttggtagg taaattccag gtcggcattg
     1261 cggcggtaat cgttgttttc tgtgccgatg tcttgccggt tgaggaacat ggccttgatg
     1321 ttggagcggg cgaagagttt gcgctggaaa gctgcggcga agtagttttg tgccggttcg
     1381 ccgtttttgc cgccggtttg catgtccatg aggccgatgc gccagttttt gtccaaattg
     1441 ccgctgagcc ttgcgccgaa caaaatgggc acgggctgca cgtttttatc taacccgatg
     1501 gtgcgcgaaa aaaacggacg gatgggcggc acgccgaagc cggcgaagag atcgctgttt
     1561 tcgaggaaga aattgcgtcg ttcgggaaag aagatattga agcgggtgag gttggtaacc
     1621 tgttgatcta cttctatttg tgagaaatcc gggttgacag tcaggtcgag gttgagggca
     1681 gccgagaggg ccactttggc atcagcgccg aggcccccgg tgaaggaggc cggcgttttg
     1741 ttttcaaaat ccacgtttgt gccgccggtg aggtagggga tgagggcgat gttgcctttg
     1801 gatttttggg gcgactgatc ccagaccaag gcgcccatgt agccgaagtc cacgccgggg
     1861 tactgctgcg gcacctgcgt ccaggtgtgg tattgattgt ttttgatgtc gttgcgaata
     1921 aaattgacgc cccacacggt tttgccctcc tcgtagcgca gggttttgaa agggatggcc
     1981 atttcgaggg tccagcggtc ggcatgttgt tgtacggcgc tgaaccaccg gttgtcccat
     2041 tccatacccg gctcgtcgaa ggcctggagg aggccctcgg tttgcacgcc tgccgcactg
     2101 acgccgaaga ggaagccgta ggttttttcg ttcacaggat cgagcacgaa tgcgaagccg
     2161 tctctgtccc agaaatccac gtcgcgtttc atggtttgga tgacgtgccg accggtatcg
     2221 tagagggtag cggcgaggta gaggtagtcg tcgttccagg ccaactgcac ctcggttttg
     2281 aggtgtgctt tgacgtcgtc gcggggccat ttttgccaga agtcggcggc tttttcggcg
     2341 ctttgccaca ccggttcggt caggttgccg tcgagcgtaa tggtctcggc agtgcgcagt
     2401 atctgcacgc ggtattgttc ctggtagatg gcggcgtttt gggccaatgc atgaaatgcc
     2461 aaaacaaaca gagcggtaaa gagcaaggta ttcttcatgg tggtgtcggg tgagtgaggt
     2521 ggggcaaaga taggaagaag ttgggggggg gaggcaaatt attgatgcga tttgctatta
     2581 gaaaccagcc agttaagtag gtagtcggaa tggtttctgt cctttatgac gatagtgacg
     2641 ggtctggaat aatgatcgtc ctgtatccgc atttcgagcc tgcctttacc atccagtcct
     2701 ctgcttatga atgaagccgc aggcatattt gtaaggcgtt ttgtaataac aatctctatg
     2761 gggtaggtgt acgataaacc accgaacgcg ctgctttttc ctaagagtac aattgaattt
     2821 ttagtcaaaa tgaaatcagc agcatcaaag tcgtaattgg ttttttcaat acttggagga
     2881 aagaattttt tctgactgaa caggtcaaat cctttgatat aaatttttac gttttcatgt
     2941 atttctatct gatgtgcatc caactccaaa tatgcctttt gcccggcttt gtatattgta
     3001 aatgtcactc gataaaacca atacattaac attccgtatc ctgtgctgac gattataaca
     3061 ctgactataa tatccagcgc gcttgagtga tggcctccaa aaatcagcat tgccatagcc
     3121 agcaaatacc ataagaataa aggccaagtg aagaagactg gaagcctcgc tttttttaca
     3181 aattgcagaa atgtttccgg cctcattaaa cactttgatt agatagaact attgccccgc
     3241 tgacaccaag tcttccgaaa aaaatgaaaa ataaccaatg gctggtaacc gggacgcctc
     3301 cacgcgtccc ggttaccctt attccgttgt ctctgacaac acactgaaaa ataaatcttt
     3361 ataccccagc cctcacacaa gcccctgctc cccaagtatc tgcgccacct cctcctcgct
     3421 gatggcggca atcagcgcaa tgaccggaat ggcgataccc tgcttgtagg catnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnntacggc gggctttgat gtgggcatcg tacaatgcct
     3601 tggcttctgc gttcatgtcg tagtaattta ggattttttc cgccttctcc agaccggggg
     3661 cggtaaaatg ctcaggcaaa cggttgtttt tcaggtaata aatccattca tccagcgtgt
     3721 ccttagccac gtcgtcgaag ctgttgactt tgatgatgta gtactccggg aagagttcgt
     3781 aaggggcggt tacatcaaaa acctcccttt ggcgggcatt cacccgcagg acatcgtggg
     3841 tatggatgcc ccggaattcg gtagtaccgt gataaacata gtcgctgccc tgccccagat
     3901 caaaatagac gatgttgatg gaataaacct tgcgcacctt gtcgtactcg aaaccctcga
     3961 acatgttgtc ggtgatggtc ttactcaccc cgaagagcat ccggtggaaa tagctgctct
     4021 cgctgttgta ctgcaattct atgagaatga gttctttgtc ttcgctttcg cagaggatgt
     4081 ccacagcgtt caccttgtca gttgcgtcat ccttgttgcc ttgcgattcg ggtatattga
     4141 tgatggtaat ctgccgacgc agcagcacgg tgaggaagcc ttccaataca tcatagttgg
     4201 ctttttggcg cagcagtctt ttgatggccc agtcaaaact gactaagggg cgactatcac
     4261 tcatccgatt ctctaatttg ctacaaagct aagctgtttt tgccggtatc gcaagtggtt
     4321 tacatctaaa aaggcttaga cacgatctgt cggcagcgca gtcactataa tatcgctgat
     4381 agtgagtgcg gtctcattaa acacgttgat tagatagaac tattgtgcca ccatgaaaag
     4441 gcccgactac cccctgccta cttcggctcc cacaaagtga acaccctgga aatattgaag
     4501 ccgaaatgta tgcctccgtt gccccaggta ttggtgtttt cggctacgaa gccgcgttcg
     4561 atcatggaag tggcgttggt gaggtgcaat tggaaaacgt ggccgccggt ttctatgtcg
     4621 aagccgatgg agagcgcatt tttaaattca ggagctaagg tattgggcat cacatagatg
     4681 tactcggcgt tgagggcgat gcgcttgttg agtttatagc gcccggagat gccgagggcc
     4741 ggcacgtcgt ttttctcgtc gcgggtgcgc acgaggttgc gatgcacgag ggtaggggag
     4801 agctggaggc tgaaattgtc gttgaatttg cggcctacga tgagctgcca ggtgtagtac
     4861 aggcgcgagg tgaagaggtt ttcgcggtcg ggatcgggcc agcggagggt ttggatggcg
     4921 gcggtggcaa ccacggcggc ggtgacgggc atgttgcggg cgccggtgct ttggcgcagg
     4981 aatttgtatt tgatgaagcc gtcgtaggtt ttttcgtagc tgctgcggcc caggcctact
     5041 gcgaggttgt cggtgatgcc gagatcgagg ccgaggcgaa tggtggcatt gtccaggccg
     5101 aacatttcgt agaagccgcc gttgagcgtg ccgaagcggt gggagatttt gaaatcgagt
     5161 acgcccggag cggtattttc cagcgagtgc aggttgatga cccggttggt tttgaaggcg
     5221 gcgttggcaa agttgatttc cttgtcgtcg tcgccgagca gggagaggag atcgtcctga
     5281 gcattgagcg tcagggcggc ggcaaagagg aggacgaaaa ttggtaagcg ttggaagaac
     5341 atagctgcgt tcggatttta tggttggaga agtaaatcga aaacaaaagt aaaataaggg
     5401 gcgataaaaa aaataaagtg atcattttcg ccaaaggatt agataagtaa acgtttcatt
     5461 t
//
LOCUS       DAORLF010000328         5455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8173_length_5455_cov_2.163704, whole genome shotgun sequence.
ACCESSION   DAORLF010000328 DAORLF010000000
VERSION     DAORLF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8173_length_5455_cov_2.163704"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..2780)
                     /locus_tag="PK198_20400"
     CDS             complement(21..2780)
                     /locus_tag="PK198_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41170.1"
                     /translation="MKSILSLLAGLLFSCSLFAQKVDFKGFITDAESNALSGATITLL
                     TQDSIMRGFGTSFSDGSFKVEARTAGSYLLQISYIGYKTHWQRAEASAGQTQVSLGAI
                     RLEPSSELLPSVVISGERNPMRMGGDTIEYNSAAFKVQPGDAVEDLLKKMPGIEVERD
                     GTVKAHGERVKNVLVDGKEFFGNDTRVATQNLPAEAVDKVQVFDKKSDMAEFTGIDDG
                     REERSINLKLKEGHKHGYFGKGDAGAGSDGRFEGRFNLNRFSPSSRLSAIGMANNTNQ
                     QGFAMNDYFQFMNGGGIMMSGGNIEIGGGGIPLSTGAPQPGIRSSLAGGLNWSKDFSK
                     NTELTASYFLNRFRQDLERSTSRRSLGQGSLFSGQEDEDQLSRNFGHNFNLHARHKID
                     SFQNLVLRANGGFNTADFSSLGATQTFIDPDNRENAGLRNYFNEGSLFNLQSSLLYRR
                     RFRKQGRALMLQGSFGMNNQTQDGSLFAVNEFFTATPAYTDTLRQRQDVQDAGANYAF
                     NVSYTEPLGKRRYLELSAARQNYSNENRREFFDVLPVGETRNEALSNHFLRGYIYDRA
                     AMNFMLNRDRYKITAGIALQQSRLENKVIGENIPFNTRFARWLPSFTGEYEFSKTNRL
                     NLRYSTRFTEPTPEQLQPVVDNSDPLNVFIGNPQLQPEYIHELRAGYFLYDQFTFTSL
                     FANVSGTYTQNKITNAASIDSLFRRSITPLNVERETALRGNAQFSTPIRPLHIKMRAQ
                     INARLMQRILWVNSVANDVQDLTHGFDFALENRQKNKFDVSAGFRWEKTATSWSVSDN
                     HNQQFSNSTLYSELRYTPSKRWMLETKMDYSAYSAGAFGEPVTIPLWQAGLTHYFLKN
                     SRARVRLSVFDLLNKNQGISQRSSFNFVEQQRYNALGRYFLLSIGYSLSGFEKQPGIE
                     IKMEG"
     gene            complement(2847..3605)
                     /locus_tag="PK198_20405"
     CDS             complement(2847..3605)
                     /locus_tag="PK198_20405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF41171.1"
                     /translation="MKRILFFLLLQCPFWLAAQSTSGEITYRETIQMKIEMPGADEKL
                     KQMIPPSQSVTKLLIFNADATLYKEAGPAGEGDINIANESDGNSFQFVIQRPKNILYT
                     DLNKLSTINEREFFGRDFLISGKARECEWKMTGEKKMIGEYECLKAMLQDTSEQVTAW
                     FTPQIPVQTGPAGYGMLPGLILEVNVDNGTRTIMVTNLDLKPVPSIEKPSKGKKVSEK
                     EFKTIEAEKMKEMGAEMGGSGKGVRMIITSEQRN"
     gene            3760..>5184
                     /locus_tag="PK198_20410"
     CDS             3760..>5184
                     /locus_tag="PK198_20410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF41172.1"
                     /translation="MKHHRPFLIARVFTSISLLLLAGAIVWFLHKTWQDEKATLSREA
                     GFLFINTMRIIEGDALQLLVFRNINPDSTGKVEMRWSELKKPDSIEIMSFVKTERHTL
                     DNNNQLKITVESREQSDRPFNEAEGSVSMFLAMTSDSLSLRAQGLNADTTKLLLKLKT
                     GFDEALKKAGLPIRYKISRQSANEVMDTQTLPSGTYRDISSGDVYSAELSGYGSYLWL
                     KMWPQVLFSALLLACVLLAFGFFFKTLKMQQGLTETKNEFIQNISHELKTPIATMSVA
                     LEALREYDVLKPGQSAEYIDISQLELRRLSLLVEKVLGIVGLERPKARLSKTAFDLDE
                     LSAEVLVAMRPRFEQAGAQVYLHKEGASFPVEADRLHLSGVLFNLLENALKYGGDLPQ
                     ISIKLHSKPGWVCYTVKDNGSGIPREYQDKIFDTFFRAPSSNGHLVKGHGLGLSYAAK
                     VVQQHGGRIFLETSGAEGSVFTVEL"
     assembly_gap    5185..5223
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5224..>5455
                     /locus_tag="PK198_20415"
     CDS             <5224..>5455
                     /locus_tag="PK198_20415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF41173.1"
                     /translation="LYMEDEPFLAKIVREYLESRGWAVRHLPDGRGAVAAFEQFQPHI
                     CILDVMLPFDDGFSVGKSIRKRFPKTPIIYLTA"
BASE COUNT         1195 a         1399 c         1491 g         1331 t
ORIGIN      
        1 gcaagataca ttgcccctgc ctatccctcc attttgatct caatacccgg ctgcttttcg
       61 aagcccgaca acgaataccc gatgctgagc aggaagtacc gtcccagtgc attgtagcgc
      121 tgctgttcca cgaaattgaa gctgctgcgt tgcgaaatgc cctgattttt gttgagcagg
      181 tcgaatacgg agaggcgtac cctggcgcgg ctgtttttga gaaaataatg cgtcagtccg
      241 gcttgccaca gcggaatggt gacgggttct ccaaatgcgc cggcgctgta ggccgaataa
      301 tccatttttg tttccagcat ccagcgtttg gagggcgtgt agcgcagctc gctgtacaag
      361 gtgctgttgg aaaactgctg attgtggttg tcgctcaccg accagcttgt agcggtcttt
      421 tcccagcgaa aaccggccga aacatcgaac ttgtttttct gccggttttc cagcgcgaag
      481 tcgaagccgt gcgtcaggtc ctgcacatcg ttggccaccg aattgaccca caaaatgcgc
      541 tgcatgagcc gggcattgat ctgagcgcgc atcttgatgt gcaacggccg aatgggcgtg
      601 ctgaactgcg catttccgcg caaagcggtt tctcgttcca cattcagcgg cgtgatgctg
      661 cgccgaaaca gggagtcaat gcttgcggca ttggtgattt tgttttgggt gtaagtgccc
      721 gacacattgg caaacagcga cgtaaaggtg aactggtcgt agaggaagta acccgcccga
      781 agctcgtgta tgtactccgg ttgcaattgg gggttgccga taaaaacatt gagcgggtcg
      841 gagttatcca ccacgggttg cagttgttcg ggcgtcggct ctgtgaaacg ggtggagtag
      901 cgcaggttca ggcggttggt tttgctgaac tcgtactcgc cggtgaaaga gggcaaccaa
      961 cgcgcaaagc gggtattgaa aggtatattt tcgccgatca ccttgttttc cagacgggat
     1021 tgctggaggg cgatgccggc ggtgatcttg tacctgtcgc gattcagcat gaagttcatg
     1081 gcggcccggt cgtaaatata tcctctgaga aagtgattgg acagcgcctc gttgcgggtt
     1141 tcgccgacag gcagaacatc gaaaaattcg cggcgatttt cattcgaata attttgccgc
     1201 gcagcgctca gttccaaata ccgccgctta cccagcggct cggtatagct cacattgaac
     1261 gcataattgg cgcctgcatc ctgcacatcc tgccgctgcc ggagggtatc cgtgtatgcc
     1321 ggcgtagcag tgaagaattc gttgactgcg aaaaggctgc cgtcctgtgt ttgattgttc
     1381 atgccgaaag acccttgcag catcaaagcg cggccttgtt tgcgaaagcg acggcggtac
     1441 aacagggaag actggaggtt gaaaaggctg ccttcattga aataattgcg caggccggcg
     1501 ttttcccggt tatcgggatc aatgaacgtt tgggtggcgc cgaggctcga aaagtcggcc
     1561 gtattgaagc ccccgttggc acggagaacg agattttgga aagagtcaat tttatggcgg
     1621 gcatgcaagt taaaattatg gccgaaattg cggctgagtt gatcttcatc ctcttgcccg
     1681 gaaaacaaac tgccctggcc caaactgcga cgggaggtgc tgcgctctaa gtcttgccga
     1741 aagcggttga gaaaataact ggccgtaagc tctgtgtttt tggaaaaatc cttcgaccag
     1801 ttcagtccgc ccgccaggga actgcgaatg cccggctgag gcgcgcccgt gctgagcgga
     1861 atgccgccgc cgccgatctc tatgttgccg ccggacatca taatgccgcc gccgttcatg
     1921 aactggaaat agtcgttcat ggcaaagccc tgctgattgg tattgttggc catgccgatg
     1981 gctgaaaggc ggctggaggg cgaaaagcga ttgaggttga aacgcccctc gaaacggcca
     2041 tcagaccctg ctcctgcgtc gcctttgccg aagtagccat gtttatgccc ctctttcaac
     2101 ttgaggttga tgctgcgctc ctccctgccg tcgtctatgc cggtaaactc ggccatatcg
     2161 gattttttgt caaacacctg cactttatcc actgcctccg cgggcaggtt ttgggtggcc
     2221 acgcgggtgt cgttaccaaa aaattccttg ccgtccacca acacgttttt caccctttcg
     2281 ccgtgcgctt tcacggtgcc gtcgcgctct acctcaatgc cgggcatttt tttgagcagg
     2341 tcttccacgg catcgccggg ctgcaccttg aaggccgccg agttgtactc aatggtgtcg
     2401 ccgcccatgc gcatggggtt gcgttcgccg cttatgacca ccgatggcaa cagctctgat
     2461 gaaggttcca atcggatggc gccgaggctg acctgagttt ggccggcact tgcttcggcg
     2521 cgctgccagt gggttttgta cccaatgtag gagatttgca agaggtagct gcctgccgtt
     2581 ctggcttcta ctttaaagga gccgtcgctg aaagaggtgc cgaaaccccg catgatggag
     2641 tcttgcgtga gcagtgtgat ggtggcgccg gacagggcat tgctctctgc atcagtgata
     2701 aagcctttaa aatcaacttt ctgcgcaaat agagagcatg aaaaaagcag ccctgccagc
     2761 agggaaagga tagatttcat agagatgttg gtttggtggt gttcaggagc tacgagctat
     2821 catttactcg cagctccttc aaaatatcag ttccgctgtt cgctggtgat gatcatgcgc
     2881 acgcctttgc cgctaccgcc catctcagcg cccatctcct tcatcttctc tgcttcgatg
     2941 gttttaaact ccttttcaga tacttttttg cctttcgagg gcttctcaat ggagggcacg
     3001 ggtttcaggt ccaaattagt caccatgatg gtgcgcgtgc cgttgtccac attaacttcc
     3061 aggatcaggc cgggcaacat accatagccg gcagggcctg tttgcaccgg tatctgcggg
     3121 gtgaaccagg ccgtgacctg ctcggaagtg tcctgcagca tcgctttgag gcactcgtat
     3181 tcgccgatca ttttcttctc gccggtcatt ttccactcgc attcgcgggc cttgccgctg
     3241 atgagaaaat ctctgccgaa aaactcccgc tcgttgatgg tactgagctt gttcagatcg
     3301 gtgtacagga tgtttttggg gcgttgtatt acaaactgaa aagagttgcc gtcgctttca
     3361 ttcgcgatgt tgatgtcgcc ttcaccggca ggaccggctt ctttgtaaag cgtggcatct
     3421 gcattgaaaa tgagcaattt agtaaccgac tgcgaaggtg gaatcatttg tttcaatttt
     3481 tcatcggcac ccggcatttc gattttcatc tgaatggttt cccggtaggt aatctctccg
     3541 gaagtggatt gagccgccaa ccaaaaggga cattgaagca acaggaaaaa gaggatgcgt
     3601 ttcattttga acagattttt ggtaaatgat aaaataagtt ttgacgatgc aaaattagag
     3661 cgcgggctgc cggtctcagg ttaatctacc ttgatgtaag gttaattaag gttaatgacg
     3721 gtggcgggtg tcgccttttg cgcctacttt tgtaccctta tgaaacacca tcgtccattt
     3781 ctcatcgcgc gtgtgttcac ctccatcagc ctgttgttgc tggcgggggc cattgtttgg
     3841 tttctgcaca aaacctggca ggacgaaaaa gccacccttt cccgcgaggc aggttttttg
     3901 ttcatcaaca ccatgcgcat cattgagggc gatgccttgc aattattggt gttccgcaat
     3961 attaaccccg acagcacggg caaagtagaa atgcgctgga gcgaactgaa aaagccggat
     4021 tcgatagaga tcatgtcttt tgtaaaaaca gagcgccaca ctttggacaa taataatcaa
     4081 ctgaagatca cggtggaaag ccgggaacaa agcgaccgcc ctttcaacga ggccgaaggc
     4141 tccgtctcta tgtttctggc catgacgtcc gacagtcttt cgttgcgcgc tcagggattg
     4201 aacgcagata cgaccaagtt gctgctcaaa ctcaaaaccg gcttcgacga ggccttgaaa
     4261 aaggccggcc tgcccatccg gtacaagatc agccgccaat cggccaatga ggttatggat
     4321 acgcagacat tgccctccgg cacctaccgg gatatttcca gcggcgacgt ctattcggct
     4381 gaactgagcg gctacggcag ctatctgtgg ctgaaaatgt ggccacaggt cttgttctcc
     4441 gcgctgctgc tcgcctgcgt gttgctcgca ttcgggttct tttttaaaac cctgaaaatg
     4501 cagcaaggac tgacggaaac caaaaacgag tttatccaaa acatcagtca cgaactaaaa
     4561 acgcccatcg ccaccatgag cgtggccctg gaagcactgc gcgagtacga cgtgctgaaa
     4621 cccggccagt cggcagagta cattgatatt tcgcagttgg aactccggcg tctttctctt
     4681 ttggtagaaa aagtgctggg catcgtgggg ttggaacgcc cgaaagcccg cctgagcaaa
     4741 acggccttcg atctggatga actcagcgca gaagtacttg tagccatgcg gccccgattt
     4801 gaacaggccg gagcgcaagt gtacctccac aaagagggcg cctctttccc ggtagaggcc
     4861 gaccgtctgc acctgtcggg cgtgttgttc aatctgttgg aaaacgcgct caaatacggc
     4921 ggcgatctcc ctcaaataag cataaaactt cactcgaagc cgggctgggt ttgttacacc
     4981 gtgaaggaca acggctcagg catccccaga gagtatcagg acaaaatttt tgatacgttt
     5041 tttcgggcgc cttcttccaa cggccatttg gtaaaagggc acggtttggg gctgagctat
     5101 gccgccaaag tagtacagca gcacggcggg cgcatatttt tggaaacatc cggcgccgag
     5161 gggtcggtat ttacagtcga attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnntttgtac atggaggacg agccgttttt ggccaagatc gtgcgggagt acctcgaatc
     5281 cagaggctgg gcagtgcgtc atctgcccga cggccggggc gcagtggccg cttttgagca
     5341 gtttcaaccg cacatctgca ttttggacgt catgttgcct ttcgacgatg ggttcagcgt
     5401 aggaaaaagc atacgcaaac gcttccccaa aacgcccatc atttacctca ccgcc
//
LOCUS       DAORLF010000329         5447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8186_length_5447_cov_2.017062, whole genome shotgun sequence.
ACCESSION   DAORLF010000329 DAORLF010000000
VERSION     DAORLF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8186_length_5447_cov_2.017062"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>703)
                     /locus_tag="PK198_20420"
     CDS             complement(<1..>703)
                     /locus_tag="PK198_20420"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF41174.1"
                     /translation="AVCWFTHPGIFEALKKRVAAGVEVSLLLHYDEVNFRPQGLDFFA
                     LERAGAKVLAYPGPDLLHHKFAVADQRFLLSGSFNWTRAVHCDHVLWQESDALCRDFL
                     QAFKEALHRARPISALRDVPPRTLLFHHLHQPVWCSPDDIRRRNFVPRAAMLAEGLTE
                     AEWQLRQREQSLTPMPVRLWEAYWYNRQVWDEAEFRRWQADQQAPRGIRALSAYCLRL
                     RVGDVLAAVSPQQQVL"
     assembly_gap    704..713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1068..1086
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1256..>1751)
                     /locus_tag="PK198_20425"
     CDS             complement(1256..>1751)
                     /locus_tag="PK198_20425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41175.1"
                     /translation="YRVVIDSVLTELYDGVPLKKYVLESLDHFGWNRGFYLEKVGGYE
                     WFLPLGITVIPEADGPIRCYRDDEVDIHFSGLLCDYRVVSSVQDWPTNRTTTLFPNPA
                     SGYVQIESDIAPARIELYDSMGRLLFTTDQNPFTVSHLKSGVYFVQTYAGDGMRVVKR
                     LVKR"
     assembly_gap    1752..1761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1762..2154)
                     /locus_tag="PK198_20430"
     CDS             complement(<1762..2154)
                     /locus_tag="PK198_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41176.1"
                     /translation="MKTTLYIASLLSILQICPVSGQHFAPIGATWYYSSSAYGAAPVR
                     SEYYLMTAEKDSVMSGYALRKIKRTHYRYQGDSVEVAPYWLHQAGDTVSMYSPGEERL
                     YKLFIFNAIPGDTLTLDIPHEAQSGFADM"
     assembly_gap    2182..2216
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2365..3420
                     /locus_tag="PK198_20435"
     CDS             2365..3420
                     /locus_tag="PK198_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRF41177.1"
                     /translation="MAGGVGSRFWPASREARPKQFLDILGTGKSLLRLTFERFLQICP
                     PDRIYIVTHADYREQVLEHLPELSPDQVIAEPARRNTAPCIAYTALKIHALNPEANMV
                     VAPSDHIILQETAFVETIRKGLDFSARHKALLTLGIRPSRPDTGYGYIQYSSEATEGE
                     VYKVLRFTEKPPLEKAQAFIAEGNYLWNAGIFIWRAAAVLDAFRAASPGILAPLESGC
                     AVLNSPQEEDFILQTYPHLESISIDYAIMEKADNVYTIPSDFGWSDLGTWASLHAESG
                     HDEHHNVVQGSEVLLSDCSDCLVRVPEGKLAVLRGLEGYIVVDEGDVLMIYPKSKEQE
                     IKNVTKSVKENTGDRYL"
     assembly_gap    3614..3638
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3639..4798
                     /locus_tag="PK198_20440"
     CDS             <3639..4798
                     /locus_tag="PK198_20440"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41178.1"
                     /translation="KSDLILDPFCGTGTTIVEGKLHGIPSIGIEANPFAHFASTVKTD
                     WEVDVNELRTCAVQIREKALLTLKLSGISDAPSLNNLNVSNLFSIQPEKNKLILKDSI
                     SPVPLHKVMVLFDCIKAYKNKKSYQYLMLALGNSIVYEISNLKFGPEVGVGKIKPDVF
                     VVKTWYNQVVKIALDLETLNGEQNTFSEIILGDSRNIAAILNGKKISAVITSPPYPNE
                     KDYTRTTRLESVLLEFINSDKELKLLKKTLLRSNTRGVYKEDTDDHFVQNISEVQRIA
                     KEIEDRRIELGKTSGFEKLYHKVTKLYFGGMTRHLEEMKTVLRQGANLAYVVGDQASY
                     LRVMIRTGHLLGEIAESMGYELMDIVQFRTRFSTATKDNLNEEVVILRWNG"
     gene            4791..>5069
                     /locus_tag="PK198_20445"
     CDS             4791..>5069
                     /locus_tag="PK198_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41179.1"
                     /translation="MDKMAKNRYIQIIEHLFQSSFEDTLNEIEFSRSDIEQAAKQLDI
                     DLPKNLGDVLYSFRYRINLPESITKTAPDGFEWIIRPAGRSQYKFVLSN"
     assembly_gap    5070..5093
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5094..>5447
                     /locus_tag="PK198_20450"
     CDS             <5094..>5447
                     /locus_tag="PK198_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41180.1"
                     /translation="QPNPQLIETKIPDATPGIILTYTSDDEQGLLAKVRYNRLIDIFT
                     GLTCYSLQNHFRTTVPNIGQVETDELYVGLDKRGVHYIIPVQAKGGTDKIGVVQIEQD
                     FAVCAEKYPRLICRPI"
BASE COUNT         1420 a         1290 c         1288 g         1326 t
ORIGIN      
        1 cgagtacctg ctgttgtggc gacacggccg ccaatacatc gcccacccgc aggcgaagac
       61 aataggccga cagcgcccgt atgccgcgtg gcgcctgctg atcggcctgc cacctgcgaa
      121 actcggcctc atcccatacc tggcgattgt accaatatgc ctcccaaagt cggaccggca
      181 tcggcgtcaa actctgctct cgttgccgga gctgccattc tgcctctgtg agaccctcgg
      241 ccagcatcgc cgcgcgcggc acgaaattgc gccgccggat atcgtcgggc gaacaccata
      301 caggctggtg cagatgatga aacaacaaag tccggggcgg cacatcgcgc agtgcagaaa
      361 tggggcgggc gcggtgcagc gcttccttaa aagcctgaag aaaatcgcgg cacaaggcat
      421 cgctttcctg ccacagcaca tggtcgcagt gtacggcccg cgtccagttg aaagaccccg
      481 acaacagaaa gcgctgatcg gcaacggcga atttgtgatg caacaaatca ggtcccggat
      541 aagcgagcac cttggctccg gcccgctcca gtgcgaaaaa atccaatccc tgcggacgga
      601 aattcacctc gtcataatgg agcagcagcg atacttccac gccggcggct acgcgctttt
      661 tgagcgcttc aaatatgccg ggatgtgtga accagcacac ggcnnnnnnn nnntgcagcg
      721 atgcctgcgc caaggcgatg aattcgacga cctgatcttc gatgttggag aagacggccc
      781 ggggcgtgtt ggagggagtc atagttgttg aactggttgt tgtgtgatgg tttttgtaag
      841 aggcgaagtt attggttttt gaggggatgg gagggtgagg atctggattt ttgtaaataa
      901 taaaagccgt tcccgaaaag aaacagcttt tcttgcagat acgtcacttg ctgcgctact
      961 tcgaataaaa cttgggccgc ccgatgtact gcacatactg tatgaagttg aactccagga
     1021 taaacgaatg gcggcgcatg ctgatgcgcg tgtcgaaggc agcaaagnnn nnnnnnnnnn
     1081 nnnnnngata ctgcatgaag cggtacgaaa cgcccacgtt ggcatctatg cgcgcaaagt
     1141 gatacccgaa gctcacatcg ggcatgatgg taccccccag tgaggcggtt ggctaatgcc
     1201 ttcgcagtcc tgccccttta gcacctcttc cgttgagttg cttcccgctc cactctcatc
     1261 tttttaccaa cctttttact accctcatac cgtcgccggc atacgtttgt acaaaataca
     1321 cgccgctttt caaatggctg acagtgaagg ggttttggtc ggttgtaaaa aggagccgcc
     1381 ccatgctgtc atacagttcg atgcgggcag gggcaatatc cgattcaatt tgtacatagc
     1441 cggaagccgg attgggaaac agtgttgttg tgcggtttgt cggccaatcc tgcacagaac
     1501 tgaccactct gtaatcgcag agcaagccgc tgaaatgaat atcaacctcg tcatcgcgat
     1561 agcaccttat cggcccgtcg gcttccggta tgacggtgat gcccaaaggt aagaaccact
     1621 cgtatccgcc caccttttcg agataaaaac ctctgttcca accaaaatgg tccaacgatt
     1681 ccagcacata cttcttcaag ggcacgccgt cgtatagctc cgtcaagacg ctgtcaataa
     1741 ctacgcggta gnnnnnnnnn ncatgtctgc aaagccggac tgagcttcgt gaggaatgtc
     1801 gagcgtcagg gtgtcgccgg gaattgcgtt gaaaatgaat agtttataca gtcgctcctc
     1861 gccggggctg tacatcgaaa ccgtgtctcc ggcctgatgc agccaatacg gagccacttc
     1921 cacagaatcg ccctgatagc ggtaatgcgt cctttttatt tttctgagag catacccgct
     1981 catgacgctg tctttttctg ccgtcatcag gtaatattcc gacctcacag gagcggcgcc
     2041 ataagcgctt gagctgtaat accatgtcgc tccgataggt gcaaaatgct gcccggacac
     2101 tgggcatatc tgtaggatgc tgaggagaga ggcgatgtag agcgtagttt tcatacgatt
     2161 gttgttttat tttttgtgtc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaac
     2221 caagcgcttt ttctccttct gtcgtctgaa cgcacattaa cgccgggtac ttgcccgttt
     2281 cccctttctg ttataccttt gcctcgcagt ggcactcagg ccggttgcaa tatctttatg
     2341 tcgcccaata catacatcgc catcatggcc ggcggagtag gcagccggtt ttggcctgcc
     2401 agccgcgaag cgcgccccaa gcaattttta gacatcctcg gtacaggcaa gtccttgctg
     2461 cgactcacat tcgagcgctt tttgcagatt tgcccgccgg atcggattta catcgtcacc
     2521 catgccgact accgcgaaca ggtactcgaa cacctgcccg aactcagccc agatcaggtc
     2581 atcgccgagc cggcccgccg caataccgcg ccctgcattg cctacacggc cctcaaaatc
     2641 catgccctca acccggaggc caatatggta gtggcgcctt ccgaccacat catcctccag
     2701 gaaacggcct ttgtggaaac tatccgcaaa gggcttgatt tcagcgctcg ccacaaggcc
     2761 ctgctcaccc tcggcatacg ccccagccgc cccgacaccg gctacggcta tattcagtac
     2821 agctcggaag caacagaagg cgaggtgtat aaagtgctga gattcaccga aaaaccgcca
     2881 ctggaaaagg cgcaggcctt catcgccgag ggcaactacc tctggaacgc cggcatcttt
     2941 atctggcggg ctgccgctgt actcgacgcc ttccgcgctg catcgccggg cattctggcg
     3001 ccgctcgaat cgggttgcgc ggtgttgaac tcgcctcagg aagaggactt tatcctccaa
     3061 acctacccgc atttggagag catttccata gactacgcca tcatggaaaa ggccgacaat
     3121 gtttacacca ttccttccga tttcggctgg tccgatctcg gcacctgggc ctctctgcac
     3181 gccgaatcgg gccacgacga gcatcacaat gtggtacagg gcagcgaggt attgctctcc
     3241 gattgcagcg attgcttggt gcgtgtaccg gaggggaaat tggccgtatt gcgcggattg
     3301 gaaggttata tcgttgtaga tgaaggagat gtgctgatga tttatccaaa atccaaagaa
     3361 caggaaataa aaaatgtgac caaaagcgtg aaggaaaata ccggagatcg ttacctttga
     3421 gtaactgtct gtctcaggca cgaggtacta tttcttaata acccgatacc agaatgatag
     3481 acttgtttgg agaaaccagc atcagcaact acaacaagaa caagttgctt ttttcagacc
     3541 gtagctttca tgattggtat aggtttgtat tgtcgtaccc gccccattta gtaagagact
     3601 atataaagta tttnnnnnnn nnnnnnnnnn nnnnnnnnat aaaagcgatt tgatactcga
     3661 tcctttttgc ggtacgggta caacaattgt tgagggcaaa ttgcatggaa tcccatcaat
     3721 tggaattgaa gccaatccgt ttgctcattt tgccagcact gtaaaaaccg attgggaggt
     3781 agatgtaaat gaactaagaa cttgtgctgt tcaaatcaga gagaaagcgc tcttgacctt
     3841 aaaattgtct ggtatttctg atgctccatc actcaataat ttgaatgttt ctaatctttt
     3901 ttcgattcag cctgaaaaaa acaaacttat tttaaaggat tctatcagcc ctgtgccgct
     3961 tcataaagta atggttttat ttgactgtat taaagcgtat aaaaataaaa agtcttatca
     4021 gtatttaatg cttgcattgg gcaattctat tgtttacgaa ataagtaacc ttaaatttgg
     4081 acctgaggta ggagttggaa agatcaaacc ggatgttttt gtagtaaaga cctggtataa
     4141 tcaggtggta aaaattgctt tggatttgga aacattaaat ggcgagcaaa atactttttc
     4201 tgaaatcatt ttgggagatt caaggaatat tgcagccatt ctaaatggta aaaaaatatc
     4261 tgcggtgatc acttcgccgc cttaccctaa tgaaaaagat tatacccgta caactcgact
     4321 tgaatcagtt ttattggagt ttataaactc tgataaggag ttaaaattat tgaaaaaaac
     4381 attgttacga tcaaatacca gaggtgttta taaagaagat accgacgatc attttgtgca
     4441 aaatatttcc gaggtacagc gtattgcaaa agaaattgag gacagaagaa ttgaattggg
     4501 aaaaacatcc ggatttgaaa aattatacca taaagtaact aaattgtact ttggcggaat
     4561 gactaggcac ctggaggaaa tgaaaaccgt actcagacaa ggagcgaatc tggcctacgt
     4621 tgtaggcgat caggcctctt atttgcgggt tatgattaga acagggcatt tgttgggaga
     4681 aatagccgaa tctatggggt acgaattgat ggacatagtg cagtttcgca cccgattttc
     4741 aacggcaaca aaagacaact taaatgaaga agtagtgata cttcgctgga atggataaaa
     4801 tggcaaaaaa tagatacata cagataatag aacacttatt tcaaagctct tttgaagata
     4861 ctttgaatga aattgaattt tcccgatcag atatcgaaca ggctgcaaag caattagata
     4921 ttgatttacc taagaattta ggcgatgtcc tttattcatt caggtaccgg attaatttgc
     4981 ctgagtccat cactaaaacg gcccccgatg gctttgagtg gattattcgc cccgcagggc
     5041 ggtctcagta caaatttgtg cttagcaatn nnnnnnnnnn nnnnnnnnnn nnncagccga
     5101 atccccaatt gatcgaaaca aaaataccag atgctacgcc aggaatcatt ctcacttata
     5161 cctctgacga cgaacaaggg ctgctggcaa aagtgagata taaccgtttg atcgatattt
     5221 ttacaggttt aacttgctat tctttacaga atcattttcg gacaaccgtt cctaatatag
     5281 gacaagtaga aacagatgag ctatatgtcg gcttagataa aagaggcgtt cattatatca
     5341 tccctgttca ggccaaggga ggcacggata aaatcggggt ggttcaaatt gaacaagatt
     5401 tcgcagtttg tgccgaaaaa tacccccggt taatttgccg cccaatt
//
LOCUS       DAORLF010000330         5445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8194_length_5445_cov_1.994991, whole genome shotgun sequence.
ACCESSION   DAORLF010000330 DAORLF010000000
VERSION     DAORLF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_5445_cov_1.994991"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PK198_20455"
     CDS             complement(<1..725)
                     /locus_tag="PK198_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41181.1"
                     /translation="MPATVLKTLCAVAFFALVLLSHPAGAQPGREAPDGVLLRVEMKS
                     GDVFSGKRLAMTDTEIVIETSALGKLNLPLADVSEIREIQTGELINGRFIPFNEQAHQ
                     NLASPTAFAMSKGSVELHNFMLVFNQVGFGINDWFSLHVATELISPIASLVEGEFGGP
                     GWMLRPHFSYPVATDKLTLGGGLAIIGIPFSGKPVDVIAPYAVATLGNRNTHLNVSAG
                     WAFGKKDPSFFFPPRPTEQGRPL"
     gene            830..1855
                     /locus_tag="PK198_20460"
     CDS             830..1855
                     /locus_tag="PK198_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF41182.1"
                     /translation="MQEKLQQQLLDRILDGFPRKPDAVDALRSLLNVSKDAIYRRLRG
                     DSLLSPSEVALLARHFHISLDELVHEDSDRVYFSFSALNNAANSVEDYLLGLHQNLHQ
                     LEHLPDVKVYYTTSEIPIFYYFLFEELAVFKLYIWGRSAWDFDYLKHQPFDVRLISPH
                     AVALSREILDLYFRLDTTELWSINIFDNTLNQIEHHFLGGAIAAPEQALSLCDRLTDL
                     AAHLQMMAAQGKKFKPRQSPDHGAPFALLHNDSLYTNSTIFVDAPHLRRVFSTFGDPD
                     YLVSSDGRICDFTDQWLQRLIGKSVPISAQSEGLRLGFFHQVRRKIEATKRRILAEVD
                     TPVISPP"
     gene            complement(1839..>2630)
                     /locus_tag="PK198_20465"
     CDS             complement(1839..>2630)
                     /locus_tag="PK198_20465"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRF41183.1"
                     /translation="KTPDTLNVPRPALKPMNPVTEWVKVRGGSFSMGCLDTAECEVNQ
                     SPVRQVRLSDFSMSVTEVTRAQFAHFLNDYGAAKVKEGEYKNKALFAGAKWLIRDGAG
                     KWGVQQGMGKLPSGYVTWYGAHEFAVFYGMKLPTEAQWEFAAKGGRTNGHFHYSGSDD
                     LEEAGWYRGNSGKQPHPVRTKKPNALALYDMSGNVWEWVTDCAHPDYKGAPANGKAWI
                     TGGDCTRRMVRGGSYNLPSESSTVSSRMEARMDDTAEFIGFRVVK"
     assembly_gap    2631..2670
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2671..3688)
                     /locus_tag="PK198_20470"
     CDS             complement(<2671..3688)
                     /locus_tag="PK198_20470"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41184.1"
                     /translation="MRDIEDIQRDLKETVALGDPELVIKAIKAVLKPECPFYQDLLLA
                     EGRLREGNAAALRGDLDNENLQILYNQLRTSLVALINQIREVDILQEEAEAPAPPKVR
                     TGNILYRIPDAMQLEKEHKCIVRIAVNETLLRERLDNTEGIHTQDIRISDEMLVQLLD
                     PSDPAPFRIRAVNSEVQFIDEDTYTEWLFYVKPIMAGTYPLLLKVAVLEMVNGKERRR
                     EIVLEESVQIITEPVPQEPAQPELKSTAHQLQFGSIADDTNIYAPQPALPRTGPQPKT
                     ARRAVAGAMRLLAMIGVITVAGVVYFQSGGNKSLVDPGFTFPGDNNNLKEDTAVVVPP
                     TDADT"
     gene            3774..5279
                     /locus_tag="PK198_20475"
     CDS             3774..5279
                     /locus_tag="PK198_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41185.1"
                     /translation="MHFINPVEALQLHYSALETIDDAAIAEAKRKFAERLERQGETAL
                     QYHGRVYTKEQFEASFELLNIPNALSAFYRLYRYPRLNGLLSGDAAEGEVEYRALSDE
                     EMMAVLKPILTEKWSHAWVEAVATQNADAIKALQQTNITALGLLGDELLAPTDAYFEQ
                     KLAELEQFTAQMEANPLAPIPSDLMGAYWFQKNLSAPMMRALPQRFDRVKEAVMKKIL
                     RICKAIYKQQPAMAEGLLIYALEFLPDGDTRKLAMDVRQRIKGHRVVAPAQRAPQSGG
                     GAKTVLGVLASLILIVGFGIKVWRVMDQLSDRSSSSYNSYKLDRDEIKRQEAIVEQYA
                     IQHEERLVSDSIAYSEIGDISRPHGLAPLKHCQTPRPRLQGTMRKLTITGDENYDAIV
                     LLYNGDRNVGQVYVAAGQKAVWQGHVKGSPISTLIVFGKDWSTKQTNPCGEPGWFAGK
                     ILYAGFASYATFPYPVEFDSGHTGFELRRSQLHKTRELTQERFFELAQQYR"
BASE COUNT         1254 a         1422 c         1390 g         1339 t
ORIGIN      
        1 atcaaagggc ggccctgctc ggttggtctg ggaggaaaga aaaacgatgg gtcttttttt
       61 ccaaatgccc aacccgccga aacattgagg tgcgtatttc gattgcccag tgtagcaacg
      121 gcataaggcg cgatgacatc caccggtttg cctgaaaatg gaatgccgat aatggccaaa
      181 ccgcctccca gcgtcagctt gtctgtggca acggggtaag aaaagtgtgg gcgaagcatc
      241 cagccggggc cgccgaattc gccttccacg aggcttgcaa tcgggctgat taattccgtg
      301 gcgacatgca gactgaacca gtcgttgatg ccgaacccta cctggttgaa taccagcata
      361 aagttgtgca gttccaccga tcctttgctc atggcaaatg cggtggggct ggcgaggttt
      421 tgatgcgcct gctcgttgaa tggaataaat cttccgttga tcagctcgcc ggtttgtatt
      481 tcccgaattt ccgacacatc ggccaatgga aggttaagct tccccaaagc gctcgtttct
      541 atgacgattt ccgtgtcggt catagccagg cgtttgccgg aaaaaacatc gccgcttttc
      601 atttccactc ggagcagtac gccatcgggg gcctctcgtc cgggttgggc gccggcaggg
      661 tgggagagga gtacaagtgc gaaaaatgca acagcacaaa gggttttcaa aacagtagca
      721 ggcatggcag gtaaattatt ttcaagagaa gacgctcttt ttttaagttg cgtttgcgca
      781 aggtacggtt ttttgttttt tttgccttgc ccaaacgcct ttcgtcatta tgcaggaaaa
      841 actacaacaa caactattgg accgcattct ggacggcttt ccgcgcaaac cggatgctgt
      901 ggatgcactg cgcagcttgc tcaatgtgag caaagatgcc atctatcgcc gcctgcgtgg
      961 cgacagcctg ctctcgccct cggaagtggc gctgctggcg aggcattttc atatttcgtt
     1021 ggatgaattg gttcatgaag actccgaccg ggtttatttt tcattcagcg ccttaaacaa
     1081 tgcagccaac agcgttgagg actacctgct ggggctgcat caaaacctgc accaactgga
     1141 gcatttgccc gatgtgaagg tatattatac cacttccgaa attccgatat tttattactt
     1201 tctctttgaa gaactggcgg tttttaaact ctacatctgg gggcgcagcg catgggattt
     1261 cgactatctc aaacatcagc ctttcgatgt acgcctcatt tcgccgcatg ccgttgccct
     1321 cagccgcgaa attctcgacc tgtatttccg gcttgatacc accgaactct ggagcatcaa
     1381 cattttcgac aacacgctca atcagataga acatcatttt ctcggcggcg ctattgctgc
     1441 cccggagcag gcgctatcac tctgcgaccg cctgacggat ctggccgctc acctgcaaat
     1501 gatggccgcg cagggcaaaa aattcaagcc ccggcaatcg cccgatcatg gcgcgccctt
     1561 tgccttattg cacaacgact cattgtacac caacagtacc atttttgtgg atgccccgca
     1621 cctgcgtcgc gttttttcta ctttcggcga ccccgactat ttggtcagtt ccgacgggcg
     1681 gatctgcgat tttacagacc agtggctaca gcgcctcatc ggtaaatcag tgcccatcag
     1741 cgcccaatcc gaaggtctgc gtttaggttt ttttcatcag gtgagacgaa aaatcgaagc
     1801 gaccaaaaga cgcattctgg cggaagtgga tactcctgtt atttcaccac cctgaaccct
     1861 ataaattcag cagtgtcgtc catccgggct tccatgcggc tgctcacggt ggaagattcc
     1921 gagggcaaat tgtagctgcc tcccctcacc atgcgccggg tacaatctcc gcctgtgatc
     1981 caggctttgc cattggcagg ggcgccttta taatcgggat gcgcacaatc ggtgacccat
     2041 tcccacacat tgccgctcat atcgtacaat gccagcgcat tcggtttctt agtgcgtaca
     2101 ggatgcggtt gtttgccgga attgccgcga taccagcctg cttcttccaa atcatcgctg
     2161 ccggagtagt ggaaatggcc attagttcgg cctcctttgg cggcaaattc ccattgcgcc
     2221 tcggtgggca atttcatccc ataaaaaaca gcaaattcgt gtgcgccgta ccaggtgacg
     2281 taacccgaag gcaatttccc cataccttgc tgcacacccc atttcccggc gccatccctg
     2341 ataagccatt tggcgcctgc aaacaatgct ttgtttttat attcgccttc ttttactttg
     2401 gcggctccgt aatcgttcag aaaatgagca aattgagcgc gggttacttc tgtaacgctc
     2461 atagaaaaat cgctcaacct gacttgccgc accgggcttt ggttcacctc acattctgct
     2521 gtgtctaaac atcccatact gaaactgccg cctcgcactt ttacccattc cgtgaccgga
     2581 ttcattggct tgagagcagg gcggggtacg ttgagtgtat ctggtgtttt nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgtatcggc gtctgtcggc ggtacaacaa
     2701 ccgcagtgtc ttcttttaaa ttgttgttat cgccggggaa agtgaatccg ggatccacca
     2761 atgacttgtt gccgccgctt tgaaaatata ctacgcccgc cacagtgata acgccgatca
     2821 tggccaacag ccgcatggcg ccggccactg ctctgcgggc ggtcttgggc tgcggaccgg
     2881 ttctcggcag tgccggttgc ggcgcgtaaa tattggtatc atctgcaata gagccaaact
     2941 gaagctggtg cgcggtggat ttcaattcgg gttgcgccgg ttcctgtggt accggttcgg
     3001 taatgatttg cacagattct tccagcacta tttcccggcg gcgttctttt ccgttcacca
     3061 tttccagaac ggctactttg agcaacaaag ggtaggtgcc ggccattatg ggcttgacgt
     3121 agaacagcca ctccgtgtag gtatcttcat caatgaactg cacctcgctg ttcacggcgc
     3181 ggatgcggaa gggcgccggg tcggagggat cgagcaattg aaccagcatt tcatcagata
     3241 tacggatgtc ctgtgtatgg atgccctcgg tattgtcgag ccgttcccgc agcagagttt
     3301 catttacagc gatgcggacg atgcacttgt gctctttttc gagctgcatg gcgtctggta
     3361 tgcggtacaa gatgttgccg gttcggactt tgggcggagc cggggcttcc gcttcttcct
     3421 gcaaaatatc tacttctcga atctggttga tgagcgccac aagcgaggtg cgcagttgat
     3481 tgtatagaat ttgcaggttt tcgttgtcga gatcgccacg gagcgcagcg gcattgccct
     3541 cgcgcaatcg gccttcagcc agcaacaaat cctgataaaa cgggcattcg ggcttcaata
     3601 cagctttgat tgcctttatg acaagctcag gatcgcccaa ggctaccgtt tctttcagat
     3661 cgcgttgtat gtcttcgatg tctctcatac gggtgggtaa gggaatacaa agaaagggaa
     3721 agttttgata tatatgagaa tcttccgtat tttgttgccc aaaataagct gccatgcact
     3781 tcatcaatcc cgtcgaagcc ctgcaactcc actattccgc gctggaaacc atagacgatg
     3841 ccgccatagc cgaggcaaaa cgaaagtttg cagaacgcct tgaacgtcag ggagaaacag
     3901 cgctgcagta ccacggacgg gtttatacca aagagcaatt tgaagcgagt tttgaactgc
     3961 tcaatattcc caacgctttg tccgcttttt accgcctgta tcgctatccg cgcctcaatg
     4021 gattgctgag cggagatgcg gcagagggcg aagtagagta ccgcgcgctc tccgacgaag
     4081 aaatgatggc cgtactgaag cccattttga ctgaaaaatg gagccatgcc tgggtggagg
     4141 ctgtagctac tcaaaatgca gatgccatca aggcgcttca gcaaaccaac ataaccgctc
     4201 tgggcttgct gggagatgag cttctggcgc cgactgatgc ttattttgag caaaaattgg
     4261 cggaattaga gcagttcaca gcgcagatgg aggccaatcc cctggcgccg attccttccg
     4321 atttgatggg agcttattgg tttcaaaaaa acctcagcgc gccaatgatg cgggccttac
     4381 cacaacgctt cgaccgggta aaagaggccg taatgaagaa aatcctgcgc atttgcaagg
     4441 ccatttacaa acaacagccg gctatggcag agggcttgct gatttacgcc ttagagtttt
     4501 tgccggatgg agatacccga aaattggcaa tggatgtgcg ccaacgcatc aaaggccata
     4561 gagtcgtggc gcctgcccaa cgcgcgcctc aatcgggtgg cggtgcaaaa actgttctgg
     4621 gtgttctggc ttctctgata ctcatcgtcg gttttggtat caaagtatgg agggtcatgg
     4681 atcagttgtc tgacaggtct tcttcctctt acaactctta caaacttgac agagatgaaa
     4741 taaaaaggca ggaggccatc gtcgagcaat acgccattca acatgaagag cggcttgtat
     4801 ccgacagcat cgcctactcc gaaataggcg acatcagccg cccgcatggc ctggcgccgc
     4861 tgaaacattg ccagacgccg cgcccccgcc ttcaaggtac catgcgaaaa ctgaccatta
     4921 ccggagatga aaattacgac gccatcgtat tgttgtacaa cggcgaccgg aacgtagggc
     4981 aagtgtatgt ggcagcaggg caaaaggccg tttggcaagg gcatgtaaaa ggcagcccta
     5041 tttctacgct catcgttttt ggtaaagact ggagtaccaa acaaaccaat ccttgtggcg
     5101 aacctggttg gtttgccggc aaaattctgt acgccggctt tgcttcctac gccacgtttc
     5161 catatcccgt cgagttcgat tcgggccata ccggttttga gctgcgtcgt tcccaattgc
     5221 acaaaaccag agaattgacg caggaacgtt tttttgaatt agcacaacaa tacaggtgaa
     5281 agtacatcac aacctttgca gcgccgtagt agaagcgctg gagcaaatat tcggtcacgg
     5341 cggcatgtac gccgacaaag tgctggaaaa tgttttaagc tccgatcccc ggcgaggggc
     5401 cagagaccgg gctttcattg ctgaaaacac ctacgaaatt gttcg
//
LOCUS       DAORLF010000331         5444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8198_length_5444_cov_2.043607, whole genome shotgun sequence.
ACCESSION   DAORLF010000331 DAORLF010000000
VERSION     DAORLF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8198_length_5444_cov_2.043607"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PK198_20480"
     CDS             complement(<1..754)
                     /locus_tag="PK198_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41186.1"
                     /translation="MNKTFQTAIAALPAFLLTVQLLSQSAGFSISGGANYSLLQSPDA
                     QVLIPFSQFVNGKFKTYDVLSESAFEFRPGPGAFLSGGVQWNISRSLLIGTGLTLQYS
                     SYDLRPDFLRYLPAPGGSDTLFLPPTTISPTCDEIVIPDDFDPATDPFYRHDVLHLQI
                     PLELRLRPGKGQLEIAAGAWAALPAFTCIAKEDVFINRRYFNNATGVTVSECVYSKGT
                     SVENSGDGFNNLIFGVRGELAYRFTPSVGMFAG"
     assembly_gap    877..886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <887..1107
                     /locus_tag="PK198_20485"
     CDS             <887..1107
                     /locus_tag="PK198_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41187.1"
                     /translation="GVELSALGGLLREGAEGSDDAGGATEAPDGAFVSARAPARPKNN
                     HLAAPTTVISRADGSAGACFQRLKSPVP"
     gene            complement(1159..>1631)
                     /locus_tag="PK198_20490"
     CDS             complement(1159..>1631)
                     /locus_tag="PK198_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRF41188.1"
                     /translation="KILHSVMSVNDRQRHILAHKIKARLGNDLSGKTIAVWGLAFKPN
                     TDDIREAPALYIIDELLEAGAAVKAYDPEAMSNVERIYGSRITFVKDPYEALIEADAL
                     AIVTEWSVFRTPAFEVLRRLLRQPNIFDGRNLYDLDFMREQGFYYESIGRNTVQ"
     assembly_gap    1632..1641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1642..>2438)
                     /locus_tag="PK198_20495"
     CDS             complement(<1642..>2438)
                     /locus_tag="PK198_20495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRF41189.1"
                     /translation="VTGTCFAETGNHVICVDIDAAKVERMRNGEVPIFEPGLEILFER
                     NSKQGRLEFTTDLAPAVAHAQVIFLALPTPPGEDGSADLSYILGVARQLSGMIQNYTV
                     IVDKSTVPVGTSEKVHAILAENLSTELFDVVSNPEFLREGVAVEDFMKPDRVVVGTSS
                     PRAVKVMKQLFEPFVRQGNPILFMDERSAEMTKYAANAYLATRITFMNEIANLCERVG
                     ANVDMVRMGMGSDTRIGKRFLFPGVGYGGSCFPKDVQALAKTAGEYN"
     assembly_gap    2439..2557
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2558..>2869)
                     /locus_tag="PK198_20500"
     CDS             complement(<2558..>2869)
                     /locus_tag="PK198_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41190.1"
                     /translation="PPYYYLLATGSYELESWGFTQKNFRLFVADNYSQYKVWQELTHI
                     LPGIESFKPVWAGDLDQDGKPDLILECTTFNAVQDILLLSSEARPGELLRPVAVYVHP
                     P"
     assembly_gap    2870..2907
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2908..3521)
                     /locus_tag="PK198_20505"
     CDS             complement(<2908..3521)
                     /locus_tag="PK198_20505"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF41191.1"
                     /translation="MHSHLIRLLFLLLLIACIHTLPAQTSLPLDEWMVIASSLRLRAE
                     PDAQADVLTTIPNGAFVAHISSLQSGKLLTVNGLKGYWMKVRYQEQEGFVFSAYLSGK
                     YVLLNEGATLQNAPVVKYWYGIYYTETGEMLRPISVRFDTVKYDDGAFAHLVLKTNQS
                     APAIFIIGLNKPLSSRQAGIWAATVGALWNDPDYIRAGDQTALP"
     gene            complement(3688..5367)
                     /locus_tag="PK198_20510"
     CDS             complement(3688..5367)
                     /locus_tag="PK198_20510"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41192.1"
                     /translation="MPKLSPLGSSDFKKIIETGRYYVDKTAMVRAVVEGAEIQLHCRP
                     RRFGKTLNLSTLRYFFDCEGEYGHLFEGLAVTDDEEMMTRYQGKYPVIELSFKDVKMG
                     TYTTAMQAMTTLLYEEWARHTSLAGHPVYDKLMAKMETSMDTGQPAEHIFWDAIKKLA
                     DLLHQHHGAQVVLLIDEYDTPLVDAWLHGYYDDMVKFMRMLLGKGLKDNNALFKGVLT
                     GILRVAKESLFSGLNNFISDAGLKVGRYSDKFGFTEQEVKTMLAHYGLNGREMDEITA
                     WYDGYRYGGTPIYNPWSILNYVAEGGQSPSPYWVNTGGHDLLKRLFFGREADVKEQLT
                     TLMRGKSLCAEVDEFLTFRGLETNPNAILSLMYFAGYLRIGKQETVDLITEYELAIPN
                     REVLLAYRSTLMAWFNEDLGRSFNDKLLEALTTGDVLTFGEDLAKFVLKVVSFYDTAD
                     NRTEHFYHAFLLGLLARLDSAYLIRSNGESGHGRYDICLIPKDFSRKGIVMEIKAVRP
                     KRGETLPGRLTEARTQVLAKKYDTELNAHGVTDILRLAIAVEGKEVVVEQV"
BASE COUNT         1134 a         1326 c         1622 g         1185 t
ORIGIN      
        1 agccggcaaa catgcctacg gagggcgtga aacggtaggc caattcgccc cgaacgccaa
       61 agatcaggtt attgaatccg tcgccggaat tttccacgct ggtgcctttg ctgtaaacac
      121 actcactgac ggtgacgccg gtcgcattgt tgaaatagcg gcgattgatg aagacatctt
      181 ctttggcaat gcacgtgaag gcaggcaacg cagcccaggc gccggcggct atttccaact
      241 gtcctttgcc ggggcggagg cgcagctcaa gcgggatttg caggtgcagc acatcgtggc
      301 gataaaaggg atcggtggcg gggtcgaagt cgtcggggat gacgatttcg tcgcaggtgg
      361 gggaaatggt ggtgggaggc agaaacaggg tgtcgctgcc gccgggcgcg gggaggtagc
      421 gcaggaaatc gggcctcaga tcgtagctgc tgtattgcag ggtaagcccg gtgccgatga
      481 gcaggctgcg gctgatgttc cactgcacgc cgccggagag gaaagcgccg gggccgggcc
      541 gaaactcgaa ggcgctctcg gagagtacgt cgtaggtttt aaatttgccg ttgacaaact
      601 gtgaaaacgg aatgagtacc tgcgcatcgg gcgactgcaa gagggagtaa ttggcgccgc
      661 ccgatatgga aaatccggcg gattgtgaga gcaattgaac cgtgaggagg aatgccggca
      721 gtgcggcgat ggcggtttgg aatgttttgt tcataaggaa gcaggttgac gaggtgtgaa
      781 aaagaaatga gcgtccacat aacgcctgat ggcctgtgat ggtgtagagg cgggcggttt
      841 attaacattg ttagagccga caactctcta cctgtannnn nnnnnncggg tgtggagctg
      901 tcggctcttg gcggtctgct gcgtgagggg gccgaaggca gtgacgacgc aggaggagcg
      961 actgaggcgc ccgacggcgc ttttgtttct gccagagcgc cggcacgccc caaaaacaat
     1021 caccttgcag cgccgacaac tgtcattagt agagcggatg gctcggccgg cgcatgtttt
     1081 cagcgactaa agtcgcccgt accgtaggct ggtgctgtag gcccttgttt cggcgactga
     1141 agtcgcccgt accggaggct actgcaccgt attgcgtccg atgctttcgt agtagaagcc
     1201 ctgctcgcgc atgaagtcga ggtcgtagag gttgcgcccg tcgaagatgt tgggttggcg
     1261 gaggagacgg cgcagcacct cgaaagcggg ggtgcggaac acgctccact cggtgacgat
     1321 ggccaaggcg tcggcttcga tgagggcctc gtaggggtct ttgacaaagg tgatgcggct
     1381 gccgtagata cgctcgacgt tgctcatggc ttcggggtcg taggctttga cggcggcgcc
     1441 ggcttcgagc agttcgtcaa tgatgtagag tgcgggcgct tcgcggatgt cgtcggtgtt
     1501 gggcttgaag gccaggcccc acacggcgat ggttttgccg ctgagatcgt tgccgagcct
     1561 ggctttgatt ttgtgggcga ggatgtgccg ttggcggtcg ttgacagaca tgaccgagtg
     1621 gaggattttg annnnnnnnn ntagttgtat tcgccggcgg ttttggccaa tgcctgcacg
     1681 tctttgggga agcaggagcc gccgtagcct acgccgggaa agaggaagcg cttgccgatg
     1741 cgggtgtcgc tgcccatgcc catgcgcacc atgtccacat tggcgcccac gcgctcgcag
     1801 aggttggcga tttcgttcat aaaagtgatg cgggtggcta agtaggcatt ggcggcgtat
     1861 ttggtcattt cagccgagcg ctcgtccatg aagaggatgg ggttgccctg ccgcacaaag
     1921 ggttcgaaga gttgtttcat cactttgacg gcacgagggg aggaggtgcc taccaccacg
     1981 cggtcgggtt tcataaagtc ttcaacagca acgccttcgc gcagaaattc gggattggag
     2041 accacgtcga agagttctgt gctgagattt tcggcgagga tggcgtgtac tttttcggag
     2101 gtgccgacgg gtacggtgct tttatccacg atgacggtat agttctggat catgccgctc
     2161 agttgccgcg ctacgccgag gatgtatgac aggtcggcgc tgccgtcttc gccgggaggg
     2221 gtgggcaagg ccaaaaaaat gacttgggcg tgggctacgg ccggggccag gtcggtggtg
     2281 aattcgaggc ggccttgttt ggaattgcgc tcgaagagga tttcgaggcc gggttcaaaa
     2341 atggggactt cgccgttgcg catgcgctct actttggcgg cgtctatgtc cacacaaatg
     2401 acgtggttgc cggtttcggc aaaacaagtg ccggtgacnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc ggcggatgca catacactgc
     2581 taccgggcgc aacaactctc cggggcgggc ctccgaactg agcaggagta tgtcctgaac
     2641 ggcattgaag gttgtgcatt ctaatatcaa atccggtttg ccgtcctgat ccagatcgcc
     2701 ggcccaaacg ggtttaaaac tttcgatgcc cggcaagatg tgggtcagtt cctgccagac
     2761 tttgtactgg gagtagttgt cggccacaaa gaggcgaaag tttttttgtg taaaacccca
     2821 ggactccaat tcgtagctgc ccgtcgccag caagtaataa tatggcgggn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnatc ggaagcgctg tttgatcgcc cgcccggatg
     2941 tagtcggggt cgttccaaag cgctccgaca gtggcggccc agattcctgc ctgccgggat
     3001 gacagcggct tgttgaggcc gatgatgaaa atagcggggg cgctctgatt ggttttcaaa
     3061 accagatgag cgaaagctcc atcatcgtat ttcactgtgt caaaacgcac gctgatgggg
     3121 cgcagcattt cacccgtttc ggtgtaataa attccatacc agtactttac aacaggggcg
     3181 ttttgcaatg tcgctccttc gttcagcagt acatatttcc ctgacagata agccgaaaat
     3241 acaaagcctt cctgctcttg atagcgcact ttcatccagt atcctttcaa gccattgacg
     3301 gtcagcagct tgccagactg aagacttgaa atatgtgcca cgaatgctcc gttgggaatg
     3361 gtcgtcaata catctgcttg agcgtcgggt tcggcgcgca ggcgcagcga ggaggcaatg
     3421 accatccact cgtccaaagg cagtgaagtt tgggcaggaa gtgtatgaat acaggcaatc
     3481 agcagcagta aaaacagcag tcggataaga tgagaatgca taggcagcga gttgttatgt
     3541 tgttgtgtga cctatcaaaa aacaagcatg cagtaggcgc gctgtggcgc gcggttctaa
     3601 gcgtgaagga acgcgaaacc ttgaacacgt aacgtgaaac acggaacccg aaacaaggcc
     3661 cgttcctgtc cgtactacta aggcgcacta aacttgctcc acgacgacct cttttccctc
     3721 cactgcaatg gccaagcgga ggatgtctgt cacgccatga gcattgagtt ctgtatcgta
     3781 ttttttagcg agtacttgag tgcgggcttc tgtcaggcgg ccgggcaagg tttcgccccg
     3841 tttcgggcgc accgccttga tttccataac gatgcccttc cgggagaagt ctttggggat
     3901 gaggcagatg tcgtagcgac catggccgct ttctccattg gagcgaatga ggtacgccga
     3961 atccaagcgg gccagcagtc ccagtagaaa ggcatggtaa aaatgctcgg tgcggttatc
     4021 tgccgtgtcg taaaaactga ccaccttgag tacgaatttc gccaagtcct cgccaaaggt
     4081 cagtacatcg cccgtggtga gggcctccag caacttgtcg ttgaaagagc gccccaaatc
     4141 ctcgttgaac caagccatga gggtacttct gtaggccagc aacacctcgc gattggggat
     4201 agccagttca tattcagtga tgaggtcaac ggtctcttgt ttgccgatgc gcaagtagcc
     4261 cgcaaagtac atcagactga gaatagcatt gggattggtc tccaatcccc ggaaggtgag
     4321 aaattcgtcc acttccgcac agaggctttt gccgcgcatc agggtagtga gttgctcctt
     4381 cacgtcggcc tccctaccaa agaaaagccg tttgagcaga tcgtgcccgc cggtattcac
     4441 ccaataagga ctcggagatt ggccaccctc agctacataa ttgaggatgg accaaggatt
     4501 atagattggg gtgccgccgt atcggtagcc atcgtaccaa gcggtgattt cgtccatctc
     4561 gcggccattg agaccgtagt gggccaacat ggtctttact tcctgctctg tgaagccgaa
     4621 cttatcagaa tagcgcccga ctttgagacc tgcgtctgag atgaagttgt tcagcccgct
     4681 gaaaaggctc tccttggcca cccgcaggat gcctgtgagt acgcctttga agagagcgtt
     4741 attgtccttc agccccttac ccaaaagcat ccgcatgaac ttgaccatgt cgtcgtagta
     4801 cccatgcagc caggcatcca ccaaaggcgt gtcgtactcg tctatcaaca gcactacctg
     4861 agcgccgtga tgttggtgca gcaggtccgc taattttttg atagcgtccc agaagatgtg
     4921 ctcggctggt tgtccggtat ccatgctcgt ttccatcttg gccatcagtt tgtcatatac
     4981 cggatgcccc gccagagagg tgtgccgcgc ccactcctcg tacagtaggg tggtcatggc
     5041 ctgcatggcg gtggtataag tccccatttt cacgtccttg aacgacagtt caatgaccgg
     5101 gtatttcccc tgataccggg tcatcatctc ctcgtcgtca gtcacggcca gcccctcgaa
     5161 gaggtggccg tactctccct cgcagtcaaa gaagtagcgc agcgtggaga ggttcagcgt
     5221 tttgccaaag cgacgcgggc ggcagtggag ttgtatctca gccccctcta caacggcgcg
     5281 caccattgca gttttgtcca catagtagcg gccagtctct atgatttttt tgaagtctga
     5341 tgaaccgagt ggcgatagct tgggcatgat ggaagcattt gactacaaag atacgaaatt
     5401 ctccagcttg gtgaagggag gacgagtgtg aggggtgagg ggtg
//
LOCUS       DAORLF010000332         5444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8199_length_5444_cov_1.749675, whole genome shotgun sequence.
ACCESSION   DAORLF010000332 DAORLF010000000
VERSION     DAORLF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_5444_cov_1.749675"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>367
                     /locus_tag="PK198_20515"
     CDS             <1..>367
                     /locus_tag="PK198_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41193.1"
                     /translation="AGYYYSIYNDFLGFNLGAELTLDPQNGRPTNVRAYRYAANSTNT
                     VTTQGFSIGMNYYFGKYYMASGNYSWNRLNKTFEDDPIIPAFNTPEHKFNISLSGRDV
                     PINLGFLKLPKLGFNINYK"
     assembly_gap    368..388
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..587
                     /locus_tag="PK198_20520"
     CDS             <389..587
                     /locus_tag="PK198_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41194.1"
                     /translation="SPQFTGQIPTYDLVDVQVNHEWKKLNSVIKIGASNVFNNKQFQT
                     YGGPRIGRLAYISFVYEFKKR"
     assembly_gap    760..944
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <945..>1793
                     /locus_tag="PK198_20525"
     CDS             <945..>1793
                     /locus_tag="PK198_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41195.1"
                     /translation="ATRLAKMGYAVAICSYRLGWNPTAQTQPERALGLIQAAYRGVQD
                     ARTAIRYFKRSVAEQGNPFAVDTSRIVLWGEGTGGYVTLATATLDKFSEIVTTTNPAG
                     KFLTDLDGNPATLEPMVNPFANGDIYGTSVGILPVNAPPLPAGDTLCYPNHAGYSSNF
                     QLCVNMGGALGDISWLEAGDPPMISYHVPTDPNAPYVDAVLIVPTTGDPIVQVQGSFA
                     VIEKANMLGNNAAFADISDDWTTAAVAASATAGHPYKEGLYPFNRRTNAFNRQEGSPW
                     SWWNAAY"
     assembly_gap    1794..1803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1804..>2126
                     /locus_tag="PK198_20530"
     CDS             <1804..>2126
                     /locus_tag="PK198_20530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41196.1"
                     /translation="HPSGAGSYHFIGLLGNATMSAAQGRLYTDTIMGYFAPRAYAALN
                     LTSSTQELIAGEQVQLKAFPNPAGEEILIQTAAEFPMQDIRLFDMNGRQVQAHTGINN
                     NQHRV"
     assembly_gap    2127..2163
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2411..3061)
                     /locus_tag="PK198_20535"
     CDS             complement(2411..3061)
                     /locus_tag="PK198_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF41197.1"
                     /translation="MIKGSNIHKSYDNLHVLKGVDIEIGKGEVVSIVGKSGAGKSTLL
                     HILGTLDRADEGTVSINGTEITGLSGRRLSEFRNRNIGFVFQFHHLLPEFTALENVCI
                     PAFIAKTPEVQARKRAAELLDYLGLSERITHKPGELSGGEQQRVAVARALINSPLVVF
                     ADEPSGNLDTVSSQELHQLIFQLRKEFNQTFVIVTHNQELAAMSDRRLEMKDGNFI"
     gene            3075..4046
                     /locus_tag="PK198_20540"
     CDS             3075..4046
                     /locus_tag="PK198_20540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF41198.1"
                     /translation="MKLKDSARFRNFAAMMLPSDTNTVQPLLGTAMWGWTVSRPVCFD
                     LLDTFYAAGFRQIDAAVNYPINKKPDDFRAAEHILLEWIHAHGIQDLKITMKLGSLNN
                     LRSPEHNLSKSFLLLNLDDYRYRLAENWDTLMIHWDNREEPEAITETMEALAVAREAG
                     LRIGLSGIKRPDVYARLNAQAGFNFDFHIQIKHNFLQSDHARYGDFHGRRRFSAYGIH
                     AGGLKPDVNDYRSDSSLKARGGDVQTLMTELEQWKQLIEKQPRPLGFHEAAMMFAAAH
                     PDMEGIVIGPSSPAQLLDTIATWKNLDTEACDILYSGYCRLRSINPG"
     gene            complement(4052..>4687)
                     /locus_tag="PK198_20545"
     CDS             complement(4052..>4687)
                     /locus_tag="PK198_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41199.1"
                     /translation="IPVTDAPPSKVEELGKTVLDTAEKVGAKVMEQGGEALEKVMGAA
                     EQLGEKVLPVMEDAGAKIMHVAEDIGGKIFEKGGETLERAKALGGDLMEKASGLMERA
                     QEEAAKENMDDAIRQAQSMGEEAARKASATDFTKPPIDTKDSLFDKHESFFDRAARFA
                     DGDYSSKPEIRRDPDYTPAPKSEGSVSGFFDNDGDGNELIDDAIIDDSDEK"
     assembly_gap    4688..4725
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4726..>4933)
                     /locus_tag="PK198_20550"
     CDS             complement(<4726..>4933)
                     /locus_tag="PK198_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41200.1"
                     /translation="ESARETAEVVTEKAWDSTSEFLNKAMNKAEEIGEVAKEKATDIW
                     DSLMDKGETIVPEPPAPQPQSSFQD"
     assembly_gap    4934..5216
                     /estimated_length=283
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5217..>5444)
                     /locus_tag="PK198_20555"
     CDS             complement(<5217..>5444)
                     /locus_tag="PK198_20555"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRF41201.1"
                     /translation="DASQQVFAGEPVVWTSDFSNLLPGDFLFFGNFRPDGSRRISHVG
                     IYTGKGRFIHSGSDNGSIQEQSLRPGDADFQ"
BASE COUNT         1113 a         1387 c         1196 g         1174 t
ORIGIN      
        1 acgccggcta ttattacagc atttacaacg actttctggg cttcaacctc ggcgccgaac
       61 tcaccctcga tccacaaaac ggccggccca ccaatgtgcg tgcctaccgc tatgcagcca
      121 attctaccaa caccgttact acgcagggct tctccattgg tatgaattac tacttcggca
      181 agtattacat ggcttccgga aactactcct ggaaccgcct caacaaaacc tttgaagacg
      241 accccatcat tccagccttc aatacgccgg aacacaaatt caacatcagc ctcagcggca
      301 gagacgtgcc catcaatctc ggatttctga aactgcccaa gttgggcttc aacatcaatt
      361 ataagtgnnn nnnnnnnnnn nnnnnnnnat cgccgcagtt caccggccaa atccccactt
      421 atgatctggt ggatgtgcaa gtgaaccacg aatggaaaaa attgaatagc gtcatcaaaa
      481 tcggagcttc caatgtgttc aacaacaagc agttccaaac ctacggcggg ccgcgtatcg
      541 ggcgattggc ctacatctcc tttgtgtatg aattcaaaaa acgataatct tttttcacca
      601 aaaacaggta cattatgaag tatttcaaca agattttact ggctgttgcc ttgttggttt
      661 ctatcgctgc tcaggctcag gagcgctatc tggaacccgt tttcaacgag gtaactgtca
      721 cacgcgacct tacctacggg gtcaacgcca ccgttttgtn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctacc cgtttggcta
      961 agatgggcta tgctgtggcc atttgcagct atcgtcttgg ctggaaccca accgcacaga
     1021 cgcaacccga acgcgcctta ggtctcattc aggcggctta ccgtggcgtt caggatgccc
     1081 gcaccgccat ccgttacttc aagcgcagtg tggccgagca aggcaatcca ttcgcagtgg
     1141 ataccagccg catcgttttg tggggcgaag gcaccggagg gtatgtgacg ctggccaccg
     1201 ccacgcttga caagttttct gaaatcgtta ccactaccaa tccggcaggt aaattcctca
     1261 ccgacctcga cggcaacccg gccacattag agccgatggt caatccgttt gccaacggcg
     1321 acatctatgg cacttcagtg ggtattttgc ctgtcaacgc tccgccgctt cctgccggcg
     1381 acacgctctg ctaccccaac catgcgggat acagttccaa cttccaactg tgtgtaaaca
     1441 tgggcggcgc gctgggcgat atctcctggc tcgaagccgg cgaccctccg atgatctcct
     1501 accacgttcc taccgatccg aacgcgcctt atgtggacgc cgtactcatc gtacccacga
     1561 ccggcgaccc catcgtacag gtgcagggca gtttcgccgt tattgaaaag gcaaacatgc
     1621 tgggcaacaa tgcggctttt gcggacatca gtgacgactg gactacggcc gctgtcgctg
     1681 cttctgctac cgcagggcat ccttacaaag aagggctgta tcctttcaac cgccgcacca
     1741 acgctttcaa tcgtcaggaa ggctctccct ggagctggtg gaatgccgct tacnnnnnnn
     1801 nnngcacccc agcggcgcgg gttcttacca cttcatcggt ttgttgggca atgcgaccat
     1861 gagcgctgca cagggccgcc tctacactga taccatcatg ggctattttg caccgcgtgc
     1921 ttacgcagcc ctcaacctga cttcttcaac gcaggaactc attgccggcg aacaggtaca
     1981 actcaaagcc ttccccaacc cggcaggcga ggaaatactg attcagaccg cagcagaatt
     2041 ccctatgcag gatatccgtc tgtttgacat gaatggccgt caggtacagg cacacaccgg
     2101 catcaacaac aaccagcacc gcgtgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnngtattta gccaaaatca gattggaacg gggtgtagtt actcaaaaga tcatgttccg
     2221 gtaggacttt agacatccat tgagaaaaaa tcgggggctg ccctgaggtt gtttttgcat
     2281 cgcaggaacg cctcggagca gcccctttgg tttcgtggca aacgcaacta gtagctcgaa
     2341 gctctcggcc tcgcttagtt aacggatttg aagcaggttt agtcacccct tcactaccct
     2401 gtgatccttt tcagataaag ttaccgtctt tcatctccaa acggcggtcg ctcatggcgg
     2461 ccaattcctg attgtgggtg acgatcacaa aagtctggtt gaattccttc cgcaattgga
     2521 aaatcaattg atgcaattcc tgagaggaaa ccgtgtccaa attgccggaa ggctcatcgg
     2581 caaaaacgac caaagggctg ttgatgaggg cgcgggctac ggccacacgt tgttgctcgc
     2641 cgccggagag ttcgccgggt ttgtgggtaa ttcgctctga aagccccaga taatcaagga
     2701 gttcggcggc gcgtttgcgg gcctgcacct ccggagtttt ggcaatgaaa gccggaatgc
     2761 acacattttc caaggcggta aattcgggca gcaggtggtg aaattgaaat acaaaaccga
     2821 tgttgcgatt gcgaaattca gagagccggc gaccgctcag accggtaatt tctgtgccgt
     2881 tgatgctcac tgttccttcg tcggcgcggt caagtgtacc cagaatatgg agcagcgtac
     2941 tcttgccggc ccccgatttc cccacaatgg agactacctc gccttttccg atttcaatat
     3001 ccaccccttt gagaacgtgg aggttatcgt aagatttatg aatgtttgag ccttttatca
     3061 cagcgttttc aggtttgaag ctcaaagata gcgcaagatt tcggaatttt gccgccatga
     3121 tgctcccttc cgataccaat accgttcaac ccctgctggg aaccgctatg tggggctgga
     3181 cggtaagccg ccctgtctgt ttcgacctgc tcgacacttt ttacgctgcc ggcttccgcc
     3241 agatagatgc cgccgtgaat taccccatca acaaaaaacc tgacgacttc cgtgctgccg
     3301 aacacatcct gctggaatgg attcatgcac acggcataca ggatttgaaa atcaccatga
     3361 aattgggcag cctcaacaat ctgcgcagcc cagaacacaa cctcagcaag agttttctgc
     3421 tcctcaattt agacgactac cgctatcgcc tcgccgaaaa ttgggatacc ctgatgatac
     3481 actgggacaa ccgtgaagaa cccgaagcta taacggagac aatggaagca ctggctgtag
     3541 cccgcgaggc cggtttgcgc atcgggctgt cggggatcaa gcgcccggat gtgtacgcca
     3601 ggctcaacgc acaagcgggt ttcaacttcg attttcacat ccagatcaag cacaatttcc
     3661 tgcaatccga ccatgcgcgc tacggcgact ttcacggccg ccgcagattt tccgcttacg
     3721 gcattcatgc aggcggtctc aaacccgatg taaatgacta tcgttcagat agttcattaa
     3781 aagccagagg cggcgatgtg caaactttga tgacggagtt ggagcaatgg aagcaactga
     3841 ttgaaaaaca gccccgaccg cttggtttcc atgaggcggc tatgatgttc gcagcagcgc
     3901 accccgacat ggaaggcatt gtcatcgggc cttcctcacc ggcgcaattg ctcgacacca
     3961 tagccacctg gaaaaatctg gacacggaag cctgtgacat cttgtactca ggctactgcc
     4021 gacttaggtc tattaacccc ggctgaatct gctatttttc gtccgagtcg tcaatgatgg
     4081 catcatcaat gagttcgttg ccgtcgccgt cgttgtcgaa aaagcccgaa acgctgcctt
     4141 ctgatttggg agcaggcgtg tagtcggggt ctctgcgaat ttcgggtttg gagctgtaat
     4201 cgccgtcggc aaagcgtgca gcgcgatcaa agaagctctc atgcttgtca aataagctgt
     4261 ctttggtatc aatgggcggc ttggtaaaat cggtggcaga tgctttacga gcagcttctt
     4321 cacccatgct ttgcgcctgc cggatggcat cgtccatatt ttctttggca gcttcttcct
     4381 gtgcgcgttc catcaggccg gatgcttttt ccatcaggtc gccccccagc gctttggcgc
     4441 gctccagcgt ttcgccgccc ttttcaaaga ttttacctcc gatgtcttct gcaacgtgca
     4501 tgatttttgc acccgcatct tccatcacag gcaacacttt ttcgcccaat tgctcggcag
     4561 cgcccattac tttttccaat gcttcgccgc cttgttccat tactttggcg cccacttttt
     4621 cggcggtatc caatacggtt ttgcccaact cttctacttt agaaggcggt gcatccgtta
     4681 ccggaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatctt ggaaggatga
     4741 ttgcggttga ggcgcaggcg gctccggcac gatggtttcg cctttatcca tcaggctgtc
     4801 ccagatgtcg gtcgcttttt ctttggcaac ctctccaatt tcctcggcct tattcatggc
     4861 tttgttcaaa aattcactgg tagaatccca ggctttttca gtgacgacct ccgccgtttc
     4921 gcgagcggat tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcct
     5221 gaaagtctgc atcgcctggg cggaggctct gttcctgaat gctgccgttg tcggacccgc
     5281 tgtgaatgaa gcgccccttg cctgtgtaaa tgccgacgtg cgagatgcgg cggctgccgt
     5341 cggggcggaa atttccgaaa aacaaaaaat ctccgggcag caaattggaa aaatcagagg
     5401 tccaaaccac cggttcgcct gcaaacactt gctgcgaggc atcg
//
LOCUS       DAORLF010000333         5436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8214_length_5436_cov_1.918602, whole genome shotgun sequence.
ACCESSION   DAORLF010000333 DAORLF010000000
VERSION     DAORLF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8214_length_5436_cov_1.918602"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>596)
                     /locus_tag="PK198_20560"
     CDS             complement(<1..>596)
                     /locus_tag="PK198_20560"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRF41202.1"
                     /translation="YGGFAGDESSIGERNIASNPTILSGEIGDSGIKSDNSYHLIFVN
                     NLAQGVVIDGFIIENAYNEWGGAAILSHGSQVAISNCTFRENTGYVGGGLNFSGGQVT
                     LEKCRFHNNQGFAGGGAIASGAGTLIANNCLFYNNSANDSGAAFFSQSAPELAFTNCL
                     FYGNQVSNYDGSAIYIADGAITLDHCTVAGNNNRRALA"
     assembly_gap    597..782
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<783..>1046)
                     /locus_tag="PK198_20565"
     CDS             complement(<783..>1046)
                     /locus_tag="PK198_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41203.1"
                     /translation="GNSITAGDPVTFTAAPVNGGAAPFYQWKKNGNNVGANGFTYTDS
                     ALINGDEITVELTSSIPCAQAPAAVSNSIVMTVADRTLYVNRD"
     assembly_gap    1047..1159
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1160..2523)
                     /locus_tag="PK198_20570"
     CDS             complement(<1160..2523)
                     /locus_tag="PK198_20570"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRF41204.1"
                     /translation="MLFTFKSLNHTKKYLLFVVALMLFFSVSAEKEETRYLEMVDADR
                     TKAEAMAAEMIAADPTNGGSTPSYQWKKNGNPVGANSSTYSDATLANGDQIRVGMTSS
                     GPCAMPPTATSNKITMTVTAANSTFITGEVAASCNDNGTVSVFFSPNVMSAAVRYVRI
                     QQNIGDYLNLSEVRVIEAYSGNNVALNKPVTSQSIYSPASPDANLVDGNTSSFMNSGA
                     PGTNQFVEIDLGGNYLLSHIEVVNRPDDGQSRARDLQLSLKDAAQNVIHTQRINAYQG
                     QNTGYATTWGTANLSSSLAPLSAVRYIRVKQNANDYMNLAELRAIEAFTGENVALFKT
                     LTAQSIFGVGVQSLTNAGLVDGDINSFTASGTSGTDEYFEIDLGATYLLDRIEIVNRG
                     DCCQSRAQNLQLMLKDGNGNVVYSQQIDAYEGQNSAHTTTWNVVDVSWMDGATTLHRS
                     GLPVG"
     assembly_gap    2694..2720
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2765..>4358
                     /locus_tag="PK198_20575"
     CDS             2765..>4358
                     /locus_tag="PK198_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41205.1"
                     /translation="MQMNIRVRLLSITLIAVSLVLSNCSPPQTEASQEHFLEDKDKQH
                     NGDYFATYPLHSPDSCIIRLKAEVPPRHQPWGCMAIWYRMPRSNRDSSFRMLELYERH
                     YPHDTVHTFSQIKRAEFYVDAAQFHEADSCLQDVEQAALRLQRILDLSDVYFLRGRME
                     VYRNNFSEARQVLFKYLELLDSRDTSFSQEHALGYMSIAASYERSQQFEKSREWFDKV
                     LNAPGDNISEDWLDKLKAQAAINVGIGYLGTNPDSGLIWAQIAEKRVKEVLKLPSPHR
                     LSYLFGRAHVELAHCDVALPYLLDAYHRRPGSHEVFGYYQYPLALGHLYLCTGRLDSA
                     EILLRESLPSPDTGNLSATYRMLGEVAARRGDYQSAWEHQKTSTHLLRAKFTADRIMA
                     AAETDARYDALEQSKRVAVLEKEHQLNRQKIWLLLALLTLLISVAVALHHRERRRQKM
                     LHQQKQLIEQEKLLAELRAQLKEQELEQSQVELQQTKDELDEAAALLELKNQLIETLE
                     LRWQKHLTSEDTDIDADKISEGT"
     assembly_gap    4359..4380
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4381..4657
                     /locus_tag="PK198_20580"
     CDS             <4381..4657
                     /locus_tag="PK198_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41206.1"
                     /translation="MRILTKEDWSLFLEKFSEQFPHFLQRIQHLHPDLTAAETRLLLL
                     LKIGFDTRQMAGMLGIADNSIWRSRHRLVKKLGLDSAKELGGYISSL"
     gene            4873..>5436
                     /locus_tag="PK198_20585"
     CDS             4873..>5436
                     /locus_tag="PK198_20585"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF41207.1"
                     /translation="MKPTHLFITTVIAMIIAACSPKVSPPAAPDVPAKEDPIEAKVEA
                     LLRKMTLEEKIGQMNQYSGFWEATGPAPKGGNQERQYDQLRNGLVGSMLNVNGAAATR
                     EVQKIVMENSRLKIPLLIGYDVIHGYKTMFPIPLGETASWDLNLMEQTARIAATEAAA
                     GGVHWTFAPMVDISRDARWGRVMEGAGE"
BASE COUNT         1252 a         1372 c         1246 g         1218 t
ORIGIN      
        1 tcgccaaggc tctgcggttg ttgtttcctg ccaccgtaca atggtctaaa gtaatggcgc
       61 cgtctgcaat atagatggca gaaccatcat aattactaac ctgattgcca taaaaaagac
      121 agttcgtgaa agcaagctca ggagcagatt gcgagaagaa agctgcaccg gaatcattcg
      181 ctgaattatt gtaaaacagg caattgtttg caattaaagt tccggctccc gaagcaatgg
      241 cgccaccgcc tgcaaatccc tgattattgt ggaatctgca tttttccagc gtgacttgcc
      301 cgccagaaaa attcaatccg ccaccgacat agccggtatt ctctctgaaa gtgcaatttg
      361 aaatcgccac ttggctaccg tggctgagga tggccgcacc gccccattca ttataagcat
      421 tttcaatgat gaagccgtca atcacaacgc cttgtgccag attgttgaca aaaatgagat
      481 ggtatgagtt gtcggatttt atgccggagt cgccaatctc tcccgacaag atggtggggt
      541 tcgaggctat gttgcgctcg cctatgctgc tttcatcccc tgcaaaccct ccataannnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnaatctctg tttacataaa gcgtgcgatc tgccaccgtc atcacaattg aattgctcac
      841 agcggcagga gcttgggcgc aagggatgct gctggttagt tctacagtga tttcatctcc
      901 gtttatgagc gcgctgtccg tgtaggtgaa gccgttggcg cctacgttat tgccgttttt
      961 cttccactgg tagaaaggag cggcgccacc attcacaggc gcggcggtga acgtaacggg
     1021 gtcgccggca gtgatggaat ttccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnc ccaccggtaa tccgctgcga tggagtgtag tcgctccatc
     1201 catccaactg acatccacga cgttccatgt agtagtatga gcgctattct gcccttcgta
     1261 cgcgtcaatt tgctgtgaat aaaccacatt gccattgcca tctttgagca tcaactggag
     1321 gttctgcgcc cggctctggc agcagtcgcc ccgattgacg atttcaatcc tgtcgaggag
     1381 gtaggtggcg ccgaggtcaa tctcaaaata ttcatctgtt ccggatgtgc cgctggccgt
     1441 gaagctgttt atatcgccat ccaccaagcc tgcatttgtc aatgactgaa ctcccacgcc
     1501 aaaaatactt tgtgccgtca aagtcttaaa cagggccaca ttttcccccg taaaggcttc
     1561 aatagcgcga agttctgcaa gattcatata gtcgtttgca ttctgcttta ctctgatgta
     1621 gcgaacggca gagagcggag caagcgacga agacaaattg gctgtacccc aggtggtagc
     1681 ataccccgtg ttttgccctt gatacgcatt gattctttgg gtgtgaatga cattctgggc
     1741 ggcatcttta aggctcaatt gaagatctct ggcccgactt tggccatcat cagggcggtt
     1801 taccacttct atatgagaaa gcaggtagtt ccctccgagg tctatttcca caaactggtt
     1861 ggttcctggt gcaccgctgt tcataaaact actcgtgttg ccatccacca gattggcgtc
     1921 aggagaggcc ggcgagtaga tgctctgcga tgtcaccggt ttattcagcg ccacattatt
     1981 gcccgaatac gcttcaatca cgcgtacctc ggagaggttc agatagtcgc ctatgttttg
     2041 ttgtatcctg acataacgca ctgctgcgga cattacgttg ggtgaaaaga atacagaaac
     2101 agttccgttg tcattacaag aagcggctac ttcgccggta ataaaggtcg agttggctgc
     2161 ggttaccgtc atggttatct tgttgctcgt cgccgtgggc ggcatagcgc aagggccact
     2221 gctggtcatt ccgacacgaa tctggtcgcc gtttgccaag gtcgcatcgg aataagtact
     2281 gctgttggcg cccaccgggt tgccgttctt cttccactgg tagctgggcg tactgccacc
     2341 attggtggga tcggcggcaa tcatctcggc tgccattgct tcggctttgg ttctatcggc
     2401 gtccaccatt tcaaggtacc gcgtttcctc cttttcggca gaaacggaaa agaacagcat
     2461 caaagcaacc acaaaaagta agtatttttt cgtgtgatta agtgatttga atgtaaaaag
     2521 catgagtatt atgttttgtt aaaaagcgaa atattgatta caaacccgga aattctttat
     2581 tgcggaatgc gtccgtgaac aggtcggcaa agttctccgg ttgcaacctt cccttgtact
     2641 cattcgactt agacaaaagc gggttccttc ttagacaaat ggcaacaaca gggnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn aatgaggcta cctttgtggg gctgtttcat accaacgctt
     2761 acttatgcaa atgaatattc gcgttcgcct gctctccatt acgctgatag ctgtgtcgct
     2821 tgtattgtct aattgctccc ctccgcaaac agaggcttcg caggagcatt ttttagaaga
     2881 caaagacaaa cagcacaacg gagactactt cgccacctac ccgctgcaca gccccgactc
     2941 ctgcattatc cgactgaaag cagaggtgcc ccctcgacac cagccttggg gctgcatggc
     3001 catctggtac cgcatgcccc gcagcaacag agattcctct ttccgcatgc tggaactata
     3061 tgagcggcac tacccccacg ataccgttca cacgttttcg caaatcaagc gcgccgagtt
     3121 ttatgtggat gccgcccagt tccacgaggc cgactcctgc ttgcaggatg tggagcaggc
     3181 ggcccttcgc ctccaacgta tattggacct cagcgatgtg tattttctga gaggccgcat
     3241 ggaggtttac cggaacaact tctcagaagc ccggcaggtt ctttttaaat atctggaact
     3301 gttggacagc cgggacacct ccttctctca agaacatgct ctggggtaca tgtctattgc
     3361 agcctcttac gaacgctctc aacagtttga aaaatcacgg gaatggtttg acaaggtttt
     3421 gaatgctcct ggagataata tcagcgaaga ctggttggac aagctaaaag cgcaggcggc
     3481 catcaatgta ggcattggtt atttgggtac caaccccgac tccggtttga tatgggcaca
     3541 aattgctgaa aagagggtaa aagaagtgct gaaactacct tctccgcaca gactttctta
     3601 cctgttcggg cgcgcccatg tagagctggc gcattgtgat gtcgccctgc cttatttgct
     3661 tgatgcctac caccgcaggc ccggctcgca tgaagtcttt gggtactacc aatatcctct
     3721 ggcattgggg catctctacc tttgcacagg ccgtcttgac tcggcggaaa ttcttctgcg
     3781 ggaatcgctc cccagcccgg atacaggcaa cctttccgcc acctaccgga tgttgggaga
     3841 agtagccgcc cgacgcggag actaccaaag cgcctgggaa caccaaaaaa caagcaccca
     3901 tctgcttcgc gccaaattca ccgccgaccg catcatggcc gctgccgaaa ccgacgcccg
     3961 ctatgacgcg ctcgaacagt ccaaacgcgt ggctgtgctg gagaaagaac atcagctcaa
     4021 tcgtcagaaa atatggctgc tgctggccct cctgacgctg ctcatcagtg tggcggtggc
     4081 cctccatcat cgcgagcgcc gaagacaaaa aatgctgcac cagcaaaaac aactgatcga
     4141 acaggaaaaa ctcctggcag aactcagagc gcaactcaag gagcaggaat tggagcaatc
     4201 gcaagtcgaa cttcagcaaa cgaaagatga gttggatgag gccgccgctc tgctggagtt
     4261 gaaaaatcaa ttgattgaaa cattggaact tcgctggcaa aagcatctta catctgaaga
     4321 cacagacatt gacgcggata aaatctcaga agggacttnn nnnnnnnnnn nnnnnnnnnn
     4381 aatgcgcatc ctcaccaaag aagactggag cctgtttttg gagaagtttt cggagcagtt
     4441 tcctcatttt ttacaacgta ttcaacatct ccaccccgat ctcaccgccg ccgaaacccg
     4501 cctcctcctc cttcttaaaa ttgggttcga tactcgccaa atggcaggta tgctgggcat
     4561 tgcagacaac agcatctggc gcagccgcca caggttggtc aaaaaactgg gcttagacag
     4621 cgctaaagaa cttggcgggt acatcagcag cctttgagcg cgggtttcgg gttcaaggtt
     4681 tcggatggga acgggcttta ttttctgatt tctgaattgc aaggttggaa cgggttgatt
     4741 tctgatttcc aacgctgaaa gttgcctgaa ctcccctttg taaagtcttt ttgggcttct
     4801 ccaagcagag aactgagaac ccgaaaccca aaaacgccta cctttacccc gcaaacaaaa
     4861 atcatttaca acatgaaacc aacgcatcta ttcatcacta ccgtcatcgc catgattatc
     4921 gcagcctgct cgcccaaagt atcgccgccc gctgcccccg atgttcctgc aaaagaagac
     4981 cccattgagg ccaaagtgga agcgctgctg cgcaaaatga cgctggaaga aaaaattggt
     5041 caaatgaacc agtacagcgg cttctgggaa gctaccggcc cggcgccaaa aggcggcaat
     5101 caagaacgac aatacgacca actccgcaat ggattggtcg gctccatgct caacgtcaac
     5161 ggcgcagccg ccacccgcga ggtgcaaaaa atcgtcatgg aaaacagccg cctcaaaatt
     5221 ccgcttctca tcggctacga cgtcatccac ggctacaaaa ccatgttccc catcccgctg
     5281 ggcgaaactg ccagttggga cctcaacctt atggaacaaa ccgcccgcat cgccgctacc
     5341 gaagctgctg ccgggggcgt tcactggact ttcgctccga tggtggacat ctcccgcgat
     5401 gcccgctggg gccgcgtcat ggaaggcgcc ggtgaa
//
LOCUS       DAORLF010000334         5429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8229_length_5429_cov_2.051172, whole genome shotgun sequence.
ACCESSION   DAORLF010000334 DAORLF010000000
VERSION     DAORLF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8229_length_5429_cov_2.051172"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2096)
                     /locus_tag="PK198_20590"
     CDS             complement(249..2096)
                     /locus_tag="PK198_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41208.1"
                     /translation="MNNRFPKGKSAMICWWVVIGMLAAWIPATSQISPGKLSKAHAHL
                     EGVANCTQCHTLGQKISNEKCLACHSEIKSRVDKKTGYHASKAIAGKECIECHSDHHG
                     VNFDMVRFDEKQFDHQLTGYPLTGAHKTTDCRQCHRPDFVGDPKLKKRTETYLGLQKA
                     CIGCHDDYHQSTLSNQCANCHTTESFAPASKFDHNKSKFQLVGKHTEVKCAQCHKTET
                     RNGKEFQVFTGLPFANCNNCHQDPHQNNLGNDCKQCHTEQSFISLTGLKRFNHSKTQF
                     PLKGAHQHVNCASCHKLDAPPAIIFQDKKGVKTEACNTCHKDAHEGKFGLQCADCHNE
                     KSFTQINTGAFNHSLTNFALEGKHRQVDCRKCHTSDRFTDPLPHNTCSACHTDYHQGE
                     FVVNNTAPDCAACHTTDGFQLSNFTIEQHARTAFPLTGAHTATPCFACHLKSENRWRF
                     RNIGIRCVDCHTDVHKGSISASYYPDQNCTVCHVTSEWKKLGQFDHSGTKFKQEGAHQ
                     RTSCKACHAGDAQYPQGRFAGTRQDCVACHENVHGRQFEQAGVTDCRRCHGFEGWGAQ
                     YFDHSKTRFPLEGKHAQVACSACHKSIELEGQMVVEYKIESFECRDCHK"
     gene            complement(2093..3418)
                     /locus_tag="PK198_20595"
     CDS             complement(2093..3418)
                     /locus_tag="PK198_20595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF41209.1"
                     /translation="MEEVIIYSILFGICASIVFLYIKSVQKSSRKVAAKIETAKEEGL
                     FEPVSLHPVIDLDICIKSSACVKACPEKDILGILQGGGTLINASNCVGHGACFHACPV
                     EAITLVIGTEKRGVDLPHVSEQYESNVEGIYIAGELGGMGLIKNSIEQGVLAVENMIK
                     AGIPKGGEGYDLIIVGAGPAGIAATLTAKKNGLNFLTIEQDTLGGTVFTFPRSKIVMT
                     SPVELPLYGKMKLYDTSKQELLDIWNQALTSNNIHIQEHTKVESIVPHKGGFHVLTQK
                     GEQLWAKNVLLAIGRRGTPRKLDVPGEVSEKVAYRLLEPEQIQGKNVLVVGGGDSAVE
                     AAMLLGTQNNVTLSYRNDKFSRIKPKNRENIMAAMERGQVHARFNTNVARITSEKVFL
                     KGEGIDGELALDNDLVYIFAGGELPTEFLQKAGIEITKRFGYTMKKHAK"
     gene            complement(3435..4304)
                     /locus_tag="PK198_20600"
     CDS             complement(3435..4304)
                     /locus_tag="PK198_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41210.1"
                     /translation="MTTSVHRLYIAIMVLTVIVVTAILAYKGVSYYSTSIEERFYHPD
                     HDQFKPSGIYGHGLGIAGTLLMLVGVTLYIVRKRWKILARLGRLKYWLEFHIFLCSLG
                     PVMILFHTAFKFGGIVSIAFWSMVAVVVSGIIGRYIYVQIPRTIEGRALSLGEVQSMK
                     LNLGELMRDTYQLPHETYGAIVSAVNSHDLVYNKNLISGIISKYFEDRRAVRSIKIDL
                     KKKGLPVKEIARLTKLISGEIRLNNRIARLQTMHKLFRYWHVAHLPFALIMLVIVLLH
                     TGITLALGYKWIF"
     gene            4419..5300
                     /locus_tag="PK198_20605"
     CDS             4419..5300
                     /locus_tag="PK198_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41211.1"
                     /translation="MKHVIQSLAFSKWLAVISIFAISCSTQSPDSKEKRPTLGDFAFY
                     RYEVRQGDSTLFQSFKEPGDTSQTFVESLDLHSGNLIMEELMKRLPTMASGDSIAFDF
                     GRGLQGRLHLLHFITKDEYPAYIAEGDKSRAAFEERLEAIKKELARLEPEYHRRAPAV
                     ADSMAYWVGELKKPEFKAALQSYNPGIQYHLIRKGDGPTANKSSSWTWVQFCAALPNG
                     EILHNSYESLPVLANRRGALMAPWIEKSVVQFPEGSLVLLVVPYELAFGEEGNVPVPK
                     GSEMYVLMEILRANNMY"
BASE COUNT         1311 a         1313 c         1287 g         1518 t
ORIGIN      
        1 ttccgacact ttattattgg gcgccaaggc attgtttttc caaaaagatg cgggtatgtt
       61 ccaactgtcg ggcagatggc aggcagagca atccattttc atcgctgctc cgtgagggct
      121 ttgcgtgaac gctgttccgc cggttagaaa gagcagaacg agtacgatag tcgcatgtcg
      181 tgacataagg ctctctattt caaaatgtaa atgaatgact ttttactaaa cacacaaaca
      241 ttttttcatt atttgtggca gtcccggcat tcgaagcttt caattttata ttctaccacc
      301 atctgacctt ctaattcaat tgatttgtgg caggccgagc acgcaacttg cgcatgtttg
      361 ccttccagcg ggaagcgggt cttgctgtgg tcgaaatatt gagcgcccca gccttcaaag
      421 ccatgacacc gacgacaatc cgtgacgccg gcctgctcaa actgccggcc atgcacattc
      481 tcgtgacagg ccacgcaatc ttggcgcgtt ccggcaaaac ggccttgggg atattgtgca
      541 tctccggcat ggcaagcctt acagcttgta cgttggtggg cgccttcctg cttgaatttt
      601 gtaccggagt gatcaaactg gcccagcttt ttccattcgg aagtgacatg acatacggtg
      661 cagttttgat cgggataata tgaagcagaa atactgcctt tgtgtacgtc cgtgtggcag
      721 tcaacgcatc ttatgccgat gtttctgaac cgccatcggt tttcgctttt caaatgacag
      781 gcaaaacaag gcgttgcagt gtgtgctccg gtgagcggaa aagcggtgcg ggcatgttgc
      841 tctattgtaa agttggagag ttgaaaaccg tcggtagtgt ggcaagcagc gcaatcgggc
      901 gcagtgttgt tgacgacaaa ttcgccctga tgatagtcgg tatggcaggc tgaacaagtg
      961 ttgtgcggaa gcgggtcggt aaatctgtct gaagtgtggc actttctgca atccacctga
     1021 cgatgtttac cttccagagc aaaattggta aggctgtggt tgaacgcgcc ggtattgatt
     1081 tgtgtaaaac tcttttcatt atgacaatca gcgcattgca gcccgaattt tccttcgtgc
     1141 gcatccttgt gacaggtatt gcaggcctcg gtttttacac ctttcttatc ttgaaaaatg
     1201 atcgcaggcg gggcatccag cttgtggcag gaggcacaat tgacgtgttg gtgcgccccc
     1261 ttgagcggaa actgcgtttt gctatgattg aatcgtttca accctgtcag cgagataaat
     1321 gactgctccg tatggcattg tttacaatca tttcccaggt tgttttggtg tgggtcttgg
     1381 tggcaattgt tgcagttggc aaacggcagg cccgtaaaga cctgaaattc ttttccgttt
     1441 cgggtttcgg ttttgtggca ttgcgcgcat tttacctctg tgtgtttgcc taccagttgg
     1501 aatttgcttt tgttgtgatc aaattttgag gccggagcga aggattcggt cgtatgacag
     1561 ttggcacact gattggacaa ggtactctgg tggtagtcgt cgtggcagcc gatgcaggct
     1621 ttttgcaagc cgaggtaggt ctctgttcgc tttttcaatt tggggtctcc cacaaaatcc
     1681 gggcgatgac attgccggca atctgtagtt ttgtgcgctc ctgtgagcgg gtaaccggtc
     1741 agttgatgat caaattgctt ctcatcaaaa cgcaccatgt cgaaattgac gccatgatga
     1801 tcgctgtggc actcgatgca ttctttgccg gcaatggctt tggatgcgtg atagcccgtt
     1861 tttttgtcca ccctgctttt gatttctgaa tggcaggcca aacatttctc attagaaatc
     1921 ttttgcccca aagtgtggca ttgggtacaa ttggccacgc cttccaaatg cgcgtgtgcc
     1981 ttgctcaatt tacccggaga aatttgggat gtggccggaa tccatgcagc caacatgccg
     2041 ataacgaccc accagcatat cattgccgac ttgccttttg gaaatcggtt gttcatttgg
     2101 cgtgcttttt catggtataa ccaaatcgtt tggtgatttc tatgccggct ttctgtaaaa
     2161 actcggttgg caactcgcct cctgcaaaaa tgtacaccaa atcgttgtcc aaagccaatt
     2221 ctccgtctat tccctctcct ttcagaaaga ctttttcgga ggtaatgcgc gccacattgg
     2281 tattgaatct ggcgtgaacc tgcccgcgct ccatggccgc catgatgttt tcccggtttt
     2341 tgggtttgat ccggctgaac ttatcgttgc gatatgagag cgttacgttg ttttgcgtac
     2401 ccaacagcat ggccgcctct actgcggagt cgccgcctcc caccaccaac acgtttttgc
     2461 cctgaatctg ctccggttcc agcagccgat aggctacttt ttcgcttact tctcccggta
     2521 cgtccagctt acgcggggtg ccccggcgac caatagcgag caatacgttt ttggcccaca
     2581 attgttctcc tttctgcgta agtacatgaa acccgccctt gtgaggcaca atggattcga
     2641 ctttagtatg ctcttgtata tggatgttgt tcgacgtgag ggcttggttc caaatatcga
     2701 gcagctcttg tttgctcgta tcgtacaact tcattttgcc gtacaagggc aactctaccg
     2761 gcgaggtcat tacaattttt gaccgaggga aagtaaaaac cgttccgccc agcgtgtctt
     2821 gctctatggt cagaaaattc aggccatttt ttttagccgt aagagtagct gcaatccctg
     2881 ccggccctgc tccaacgata atcagatcat acccctcgcc tcccttcgga atgccggctt
     2941 taatcatatt ttcaacggca agtacgccct gctcgatact gtttttgatc aatcccatgc
     3001 cgcccagttc gccggcaata taaatgcctt ctacattaga ttcatactgc tcgcttacat
     3061 gtggcaaatc aacgcctctc ttctctgttc caatgactaa agtgatggcc tctaccggac
     3121 acgcatgaaa acaggcgccg tgccccacac aattggaagc attgatgagc gtgccgcctc
     3181 cttggagaat gccgagaatg tctttttccg ggcaggcttt tacgcaggcg ctgcttttga
     3241 tgcagatgtc gaggtctatg acgggatgta gggaaacagg ttcaaagagt ccttcttcct
     3301 ttgctgtttc tatcttagca gccactttac gcgatgactt ttgtacactt ttgatgtaca
     3361 aaaacactat cgaagcgcag ataccgaata gaatactata tataataacc tcttccatct
     3421 aatttcacat cgttttaaaa aatccactta tatcctaaag caagcgttat gccagtatgc
     3481 aacagcacaa taacgagcat gatgagagca aagggtaaat gcgctacatg ccagtatctg
     3541 aacagcttgt gcatggtttg aagtcgggca atccggttgt tcaaacgtat ctcaccacta
     3601 atgagctttg tcaatcgagc tatttccttc acaggcaatc ctttcttttt caaatctatt
     3661 ttgatagacc tgacagcgcg cctatcttca aaatatttgg aaatgatacc actgatcaga
     3721 ttcttgttat acaccaaatc atgtgaattg accgcagata caatggcacc atacgtttcg
     3781 tggggaagtt ggtaagtatc gcgcatgagt tctcccaaat tgagtttcat tgactgtact
     3841 tcacccaaag acaaagcccg accttcgatg gtacgcggta tttgaacata aatgtacctt
     3901 ccaatgatgc cactcaccac cacagccacc atactccaaa aagcaatcga aacaatgccc
     3961 ccaaacttaa atgctgtatg gaagagtatc ataaccgggc caagactgca taggaaaatg
     4021 tgaaactcca gccaatattt caacctgcct aatcgagcca ggattttcca tctttttctt
     4081 acaatgtaca aagtgacccc caccaacatc aataaggtac ctgcaatgcc caaaccgtgt
     4141 ccatagattc cgctgggttt gaattggtcg tgatcgggat ggtaaaaacg ctcttctatg
     4201 ctcgtggagt agtaagaaac acctttgtaa gctaatatcg cagtcacaac gatcacagtt
     4261 agaaccatga tggcgatata caatcggtga accgaagttg tcatgtatta tttatgcttg
     4321 tttataaatt tgcggcagca aaggtaatct ttcgatcgtc aggacggtaa gtcaggtgct
     4381 gatttagaat gattctgaat tcactaacac gctataaaat gaaacatgta attcaatctc
     4441 ttgcatttag taaatggttg gctgtcatca gcattttcgc gatttcatgc tctacgcagt
     4501 ctcccgattc gaaagaaaaa cgccctacac ttggagactt cgccttctac cgctacgaag
     4561 tccggcaggg cgattccacc ctctttcaat ctttcaaaga gccgggcgat acctcgcaaa
     4621 cctttgtaga atctcttgac ctccacagcg gcaacctcat catggaggaa ctcatgaaac
     4681 gccttcccac aatggcttcc ggagacagca tcgcttttga tttcgggcgg ggtttgcaag
     4741 ggcgattgca cctgctccat tttattacca aagacgagta tcctgcatac attgccgagg
     4801 gcgacaaaag ccgggctgca ttcgaggagc gcttggaagc gatcaaaaaa gaacttgcga
     4861 gattggagcc ggagtaccac cgcagagcgc cggcagtagc cgacagcatg gcgtattggg
     4921 tgggtgaatt gaaaaaaccg gaattcaaag cagccttaca atcatacaat cctggtattc
     4981 aataccatct gattcgaaag ggcgacggcc caacggccaa taaatcatct tcctggacct
     5041 gggtgcagtt ttgtgcggcc ttgccgaatg gtgaaatttt gcacaattct tacgaatcgc
     5101 tcccggtgct ggccaaccgc cgcggtgcgc tgatggcgcc ctggattgaa aaatctgtgg
     5161 tgcagtttcc tgaaggcagc ctcgttttat tggttgttcc ttatgaattg gctttcggcg
     5221 aagaaggcaa tgtccccgtg cccaaaggca gcgaaatgta tgtgctgatg gagattttga
     5281 gggcgaataa tatgtattga tgcccgacta ccacaaaaac gggtggaatt tcactcgtcc
     5341 tctcgaagtc aattccaccg caggcgcagg cactttgatc caattgatgg tgtggaagag
     5401 ggttttcagg aggcggcttt gggttttaa
//
LOCUS       DAORLF010000335         5426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8236_length_5426_cov_1.469373, whole genome shotgun sequence.
ACCESSION   DAORLF010000335 DAORLF010000000
VERSION     DAORLF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8236_length_5426_cov_1.469373"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1379
                     /locus_tag="PK198_20610"
     CDS             <1..>1379
                     /locus_tag="PK198_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF41212.1"
                     /translation="LYLPYKRKRKTRASVAREKGLEPLAQAIFAQNNDRLESLAAPFL
                     NDQVPDVEEALQGARDIIAEWINEDERARHSTRSAFHREAVIYSKVAKGKETEGDKYR
                     DYFDWSEPLKKCPSHRLLAMRRGEEEGILRLIIKPDEEVLLGRLERHFVKGSGPAAQQ
                     VKSALTDSYDRLLAPSIETEFRQSSKERADKEAIEVFAVNLRQLLLAAPLGERRIMGI
                     DPGFRTGCKVVCLDASGNLLHNDTVYPHPPQSDVWMAKNILQSLIDRFETEAIAVGNG
                     TAGRETMDVLMSMDFGRPVQIFMVNEAGASIYSASEAGREEFPTQDITVRGAVSIGRR
                     LMDPLAELVKIDPKSIGVGQYQHDVSQPLLKEMLDQVVESCVNSVGIQLNTASKHLLT
                     HVSGLGPVLAQNIVEYRRENGPFRSRKELRKVPRLGDKAFEQCAGFLRIREGEHLLDN
                     TAVHPESYPV"
     assembly_gap    1380..1398
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1399..1828
                     /locus_tag="PK198_20615"
     CDS             <1399..1828
                     /locus_tag="PK198_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRF41213.1"
                     /translation="KDLHCTVDALAQQPELRQKIDLQKYVSDKVGLPTLQDILKELAK
                     PGLDPRGQARAFEFDRSIKSIDDLRIGMVLPGIVNNITNFGAFVDIGIKNSGLVHVSQ
                     LANRFVKNPAEVVSLHQEVMAKVIDIDLARGRVQLSLKEV"
     gene            complement(1866..>2239)
                     /locus_tag="PK198_20620"
     CDS             complement(1866..>2239)
                     /locus_tag="PK198_20620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF41214.1"
                     /translation="EIPSKGPAYTVLFFWGTWCGPCKRDHPALKQLFEKYADKGVRFI
                     GVAADKSPEHVANYIEKNKLPWFNMFVSIAEALAKAEPIINSYEVRSYPTYFLIDPQG
                     FIAASGKLGDIKESLGNLKWE"
     assembly_gap    2240..2387
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2388..>2623)
                     /locus_tag="PK198_20625"
     CDS             complement(<2388..>2623)
                     /locus_tag="PK198_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41215.1"
                     /translation="KPLMNMIPPPQNKLSEDIRISITTNEYYTGTFQVGHEKYTLRIA
                     EAFSQPSRPGRNRLSIYPYGRFRDTTVNSIDAEF"
     assembly_gap    2624..2655
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2656..>3096)
                     /locus_tag="PK198_20630"
     CDS             complement(<2656..>3096)
                     /locus_tag="PK198_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41216.1"
                     /translation="EYSAHSAFWHYIHTTPLQGLPTDADTIMYWVCPMSVRQFLYEEI
                     KRGISFDYYPNGTVYDYSGDVNTLGIKEKDYTERDIKNNIYAISALRNGRKLVIVDAN
                     NNHDLSDDVLIDVDTAMTFEDRMSSYTGSPFIMLNYEVLYQGRI"
     assembly_gap    3097..3203
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3413..3571
                     /locus_tag="PK198_20635"
     CDS             3413..3571
                     /locus_tag="PK198_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41217.1"
                     /translation="MAKSKLTELKKYLAELDEEGLRKELLKLFNKLPQVQEYYARNSW
                     AKRSGRLY"
     assembly_gap    3600..3647
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3648..3962
                     /locus_tag="PK198_20640"
     CDS             <3648..3962
                     /locus_tag="PK198_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41218.1"
                     /translation="NPSNAEIRRIIGDFEKISVFPHELADLLIYRVEVATEFADSFGG
                     LPESDYKPSVNAFEKALKIIAENRLESYFKARCEKLFQYRNLDWWYIESLMDIYRDIL
                     GK"
     gene            complement(4147..4482)
                     /locus_tag="PK198_20645"
     CDS             complement(4147..4482)
                     /locus_tag="PK198_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41219.1"
                     /translation="MELSKSDKRAARAIIRKGLMAECERGLQGAAQIINDWKSGNSDP
                     HKAYLDLYTHIADFDKGIAAQYDNLSNDSLIFALVRQLREGLLDAQELEGLSEEAREQ
                     VRRFLEWRE"
     assembly_gap    4657..4687
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4688..>5426)
                     /locus_tag="PK198_20650"
     CDS             complement(<4688..>5426)
                     /locus_tag="PK198_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF41220.1"
                     /translation="NTHHVVKALSSLELLVVQEIFMTETAKLAHVVLPGASFLEKEGT
                     FTNGERRIQRVQRVVEPVGDCKTDGQILVEMMNRLGYPQRPYTAKGMLEEISQIVPFF
                     AGVRWEELGDNGKQWPVAPDGTDTKILHQESFKRGKGRFVFREFVETKEILDYAQDFP
                     YILTTNRVLEQYNAGTMTRRTPNVQIVSEDLLLIHPQDAADNRIAQGDFVRIESARGE
                     CKVKAHLTDEVKPGILSTTFHFPELMVN"
BASE COUNT         1196 a         1322 c         1328 g         1195 t
ORIGIN      
        1 ctctacctgc cgtacaaacg caagcgaaaa acccgcgctt ctgttgcaag agaaaaaggt
       61 ttggaaccac tcgcgcaggc cattttcgct caaaacaacg accggttgga atctttggcg
      121 gcccccttcc tcaacgatca ggtacccgac gtagaggaag ccctgcaagg cgcccgcgac
      181 atcatagccg agtggatcaa cgaggacgaa cgcgcccgcc acagcacccg cagcgccttc
      241 catcgcgagg cggtcattta cagtaaggtg gccaaaggca aagagaccga gggcgacaaa
      301 taccgcgact acttcgactg gagtgagcca ctcaaaaaat gcccctcgca caggctgttg
      361 gccatgcggc gcggcgaaga ggagggcatt ctccggctca tcatcaagcc tgatgaggaa
      421 gtgttgctgg gccgtttgga acgccatttt gtaaaaggct ccggccctgc cgcgcagcag
      481 gtaaagagcg cattgacaga tagttatgat agattactgg cgccgtccat tgaaacggaa
      541 ttccggcagt cgtcgaaaga gcgcgccgac aaagaggcca ttgaagtgtt tgccgtcaac
      601 ctgcgtcaat tgctgttggc tgcgccattg ggcgaacgcc gcatcatggg catagacccc
      661 ggttttcgca cgggctgcaa ggtggtttgt ttggatgcat cgggcaacct gctgcacaac
      721 gacaccgtct atccgcaccc gccgcaatcg gatgtgtgga tggcgaaaaa catacttcag
      781 tcgctgatag atcgctttga aacagaggcg attgcagtag gaaatggcac cgccggcaga
      841 gaaacgatgg acgtgctgat gagcatggat tttgggcgtc cggttcaaat attcatggtc
      901 aatgaggcgg gggcttccat ttattcggca tcggaggccg ggcgcgagga gtttcctacg
      961 caagacatta ccgtgcgtgg cgcggtgtcc atcgggcgcc gtttgatgga ccccttggcc
     1021 gagttggtaa aaattgaccc gaaatccatc ggcgtcggcc agtatcagca cgatgtgagc
     1081 cagcctttgc tcaaagaaat gctggatcag gtggtggaaa gctgcgtcaa cagcgtcggc
     1141 attcaactca acactgccag caaacacctg ctcacacacg tctctggcct tggtccggta
     1201 ctcgcccaaa acattgtgga ataccgtcgc gagaacggcc ccttccgctc ccggaaagaa
     1261 ctgcgaaaag tgcctcgcct cggcgataaa gcctttgaac aatgcgccgg cttcctccga
     1321 atccgcgagg gagaacacct gctcgacaac accgccgtac accccgaaag ctaccccgtn
     1381 nnnnnnnnnn nnnnnnnnca aagacctgca ttgcacggtg gacgcgctgg cgcagcagcc
     1441 ggaactgagg caaaagattg atttacaaaa gtatgtgagt gataaagtcg gcctccccac
     1501 ccttcaggac attctcaagg aattggccaa acccggcctc gacccgcgtg gccaggcccg
     1561 cgctttcgag ttcgaccgca gcatcaaaag tattgatgac ctgcgcatcg gcatggtgct
     1621 gccgggcatc gtgaacaaca tcaccaattt cggggctttt gtggacatcg gcatcaaaaa
     1681 cagcggcttg gtgcatgtgt cccaattggc caaccgcttt gtgaaaaatc ccgctgaagt
     1741 ggtcagcctg catcaggaag taatggccaa agtgatagac atagacttgg ccagagggcg
     1801 ggtgcaacta tcgctcaagg aagtatgatt ttacctttaa gaccgcgtgg cgctgggcgg
     1861 gtcccttact cccatttcag attgccgagg ctctctttaa tatcccccaa ttttccactg
     1921 gcggcaataa atccctgcgg gtctatcagg aagtaggtag gataactgcg aacctcatag
     1981 ctattgatga tcggctcagc tttagcaagc gcctcggcta tacttacaaa catattgaac
     2041 cagggcagct tgtttttttc aatataattg gccacatgtt cagggctttt gtcggcggca
     2101 acgccaatga aacgaacgcc cttatccgca tacttttcaa aaagttgttt cagtgcagga
     2161 tgatcccttt tgcaggggcc gcaccatgtc ccccagaaaa acagcaccgt ataagcgggc
     2221 cctttagagg ggatttccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga attccgcgtc
     2401 aatggaattg accgtagtat ctcggaatct cccgtaaggg taaatgctca gcctgttccg
     2461 accgggtctg gatggctgcg aaaatgcctc ggctattctg agtgtatatt tttcgtggcc
     2521 cacctgaaac gtgccggtat agtattcatt ggtggtaatg gaaatgcgta tgtcttcgct
     2581 gagcttattt tgcggaggcg ggatcatgtt catcagcggc ttgnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnntgatg cgcccctgat ataaaacctc atagttgagc ataatgaaag
     2701 gcgagccggt gtaagaagac atgcggtctt caaaagtcat ggctgtatcc acgtcaatca
     2761 gtacatcgtc actgaggtcg tggttgttgt tggcatctac aatgaccaat ttgcgcccat
     2821 ttcgcaaggc cgatatggcg tagatattgt tttttatgtc tcgttccgta tagtcttttt
     2881 ctttgattcc caatgtgttg acatcgccgg aataatcgta aacggttccg ttggggtaat
     2941 agtcgaaact gatgccccgc ttgatttctt cataaagaaa ttgtcttacc gacatggggc
     3001 aaacccagta catgatggta tcggcatcgg tcggcagccc ctgaagcggc gtggtgtgga
     3061 tgtagtgcca aaaagcgctg tgggccgaat attcatnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnaaaggtg taagtgatga aggaaaaatg gatgggggcg
     3241 gctgtttccg cgccgcgggt aaaaaccggg ccggggtctt gtttattgcg tcggttgcta
     3301 atcctttgga ggcgcattgc cccggttgcg aagcctttca gtatccattt ttttctcacc
     3361 ccgcaaatct gaagccgagg aatctgcgtt tgatttccat aacctttata tcatggcaaa
     3421 aagcaaactc accgaactga aaaaatacct cgccgagttg gacgaggaag ggctgcgcaa
     3481 agaactgctc aagctgttca acaaactgcc gcaagtgcag gaatattacg ccaggaactc
     3541 atgggcgaaa aggagcggaa ggctatatta gattcgtaca agaaaaagat ttatgtccan
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat ccgagcaatg
     3661 cagaaatacg ccgcattatc ggggattttg aaaaaatatc tgttttcccc cacgaactgg
     3721 cggatttgtt gatctatcgc gtagaagtgg caacggagtt tgccgattcc tttggagggc
     3781 taccggaaag cgactacaaa ccgtccgtca acgcttttga aaaagcattg aagatcatcg
     3841 cagagaatag gttagaatcg tacttcaagg cgcggtgtga aaaattgttc caatacagga
     3901 acttagactg gtggtacatc gagagtttga tggatattta ccgcgatatt ttggggaagt
     3961 gaagcaggat acgggcatga gttgcctgct ggagagatac gaaaccgcaa tcttcgccct
     4021 gccccgagta cgtttacgaa tcctttcgga ggcttcgcaa acgtaccacc gagattacgc
     4081 tcgttatcga attttgcacg acaggacgct ttggcattta tgataggggc tgtgggagaa
     4141 gccgtttcac tcccgccatt ccaaaaatct ccgcacctgt tcccgcgcct cctccgacag
     4201 cccctctaat tcctgtgcat ccaacagccc ctcccgcagt tgccgcacca gcgcaaaaat
     4261 caagctgtca ttggacagat tgtcatattg cgcggcaatg cctttgtcga agtccgcgat
     4321 gtgggtgtac agatcgaggt acgctttatg gggatcacta ttgccggatt tccaatcgtt
     4381 tatgatttga gcggcgcctt gcaagcccct ttcacattct gccatcaagc ccttgcggat
     4441 gatggcgcgg gcggctcttt tatctgattt gctgagttcc attttacctt gtgatgcctc
     4501 tgcatttatt ttaatatgct ccagtaaccg cagatgtatt ttttctgaga attcaggagg
     4561 ccacctcgaa gtgacctcct acattgctac gcaagtacta caccctttct atccgcacgc
     4621 tcaccacctt gtactccggg caattggcga tgctgtnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnngtt gaccatcagt tccgggaagt ggaaggtcgt actgaggatg cccggtttta
     4741 cctcgtcggt gaggtgcgcc ttcaccttgc actcgccgcg agcgctctct atgcgcacaa
     4801 aatcgccctg tgcaatgcgg ttgtcggcgg cgtcctgcgg gtggatgagc aacaagtctt
     4861 cgctgacgat ctgcacatta ggcgtgcggc gcgtcatggt gccggcgttg tactgctcca
     4921 acacgcgatt ggtggtgagg atgtagggga aatcctgtgc ataatcgagg atttctttgg
     4981 tttccacaaa ttcccggaac acaaagcgcc ccttgccccg cttgaaactc tcctgatgca
     5041 ggattttcgt atccgtacca tcgggcgcta cgggccattg tttgccgttg tctccgagtt
     5101 cttcccagcg cacgccggcg aagaaaggca caatctggct gatttcctcc aacatacctt
     5161 tggccgtata aggtcgctgc ggatagccga ggcgattcat catttccacc aaaatctgtc
     5221 cgtcggtttt gcaatcgcct acgggttcca ccacccgttg cacccgctga atgcgccgct
     5281 cgccgttggt gaaagtgcct tctttttcca aaaatgaagc ccccggcagc acaacgtggg
     5341 ccagcttggc ggtttcggtc ataaaaatct cctgcaccac caacaactcc agcgaagaca
     5401 gcgccttcac cacatggtgc gtattg
//
LOCUS       DAORLF010000336         5392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8311_length_5392_cov_2.152708, whole genome shotgun sequence.
ACCESSION   DAORLF010000336 DAORLF010000000
VERSION     DAORLF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_5392_cov_2.152708"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PK198_20655"
     CDS             complement(<1..1650)
                     /locus_tag="PK198_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41221.1"
                     /translation="MKKHYIFLLLFALVTTAFADLFSQESNTDRDSYTIIYVPESKGV
                     GPLFQSELEGEYIRRFNWNTISTKYFSPSNFDIDRMAEELTSKGVGREIMSFWWQRQS
                     NGRFKLDTIAARGVFNTRIKSILDTIHDPRGIAELQDRGLNLIPNSYIVVLRPTDLIS
                     FREHYDNLEREYRKSGSSASVERSMRGFMGHLTAYVFKLKYDDQTVNDFYNMWPLDED
                     TPSVLAAKAKQYNEYKFELIPVKVYRPQDIKKGLNPIIQSAASINDSKLSDADLLKGW
                     AKTGVNNLFDKIVGRGILSNRVANNNPILADIGARDGLYPEKRYAIYEERETASGEIK
                     KTRIALVRASNRIATGNGAYSKFFRIGGVGFIKEGMLIKEYKDKGIAFQVIHGIPQIP
                     FGNQMDDWGGGVEGNLSLLIAKGNSNFPTGIKLGGNIIYSQDSLRGTGSDPVAGTGIR
                     FVLYLKKDIHFLSVFRLGLYAGIGGDSVSEKTKDANAEKNTISSTFYPVGAEFGVNIG
                     YGLSAFGQGEYIIPSGKVTLNKEEQPSLKWQDLYENRVGISL"
     assembly_gap    1806..1820
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2081..2860)
                     /locus_tag="PK198_20660"
     CDS             complement(2081..2860)
                     /locus_tag="PK198_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRF41222.1"
                     /translation="MIYDNTPTLAPQSAEALLSVQDYFDHWKWLCKYKPPRTMLLLMD
                     VAEARAIQSCGMEILGYAPGWLPGLDDIRRITHENQRHLASVHTHNVLKTLSARPELL
                     KNRKVFYCNTRGLRDHAGQYWRVYQLSTPVGFDEQGRLAAYISYYTILGPYQGEPFET
                     AVYPDPRFPADAQRLQQALNKIKSGILENLGFSRRQEELIQLMAQGASAKDIAITMRI
                     SDRTVDKHRLGVLQKGRDLFPLNAFTQAADVVGFLKKQGLV"
     gene            complement(2897..3643)
                     /locus_tag="PK198_20665"
     CDS             complement(2897..3643)
                     /locus_tag="PK198_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF41223.1"
                     /translation="MTNYSEKHRGPAISPKTTSSGPYMPGCSSGFTPVIDFQYAVNAL
                     HWRIEQGQGLAAQLGYPSAEALPPFTEELLHPEERGKIARLVRRIGDFAIQLGPDAAL
                     RSTFILAHRMKRADGSYAKILRYLQPDAMGSTGRLEHYQCFCTDVSRLPGFESVSFDV
                     RLPADVAVSTEEALLFFADVLDDSRLRFTERELQVLRVWALTNTARDAACRLDLAPRT
                     IEAHLKNMRRKLGVKRSVDVMLYARDKGWV"
     gene            3886..5307
                     /locus_tag="PK198_20670"
     CDS             3886..5307
                     /locus_tag="PK198_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41224.1"
                     /translation="MHGSTLIGLLKNLDEKSFRAFRDFVHSPYFNNRSNVVQLFEYIK
                     PCYPAFEDAKLERFMALRRLYPPGTSMQRLNDDMSKLSKLCRKFLAHNELEESPLLEK
                     KLLLDAYRRRGMTEKLSEDTERFVQQLRDEPHTDILPLRLSMEAQFEAFFYPPTEKYT
                     PFAPYLYGALHGLEQYYQLHKARIVAEILHRRHIYQEAEIPAPAAPAVLDMYRRLLDI
                     LESPKPHSGFEPLLDDIERHFASLADFDQRMLLDRLYHVANRRYEKGQPEYVPILFRI
                     IKIADQYEALTYQNAITPVALVNYAAVAGMAGDLEWGRQFVTRCQPQLTPEHREAAIH
                     LAEAHLLFYEGQFSPCLLHIGEAERYHAGMRIHAETLYLRTYCELERQKGESYLEEFQ
                     RCQKRFSKFLHHDARINKEKIKALRRFILLSSCLMQYHNRRNEKQRQKLHQQRIHKYF
                     NGADKVYSAFWLQNHIRAFVLPC"
BASE COUNT         1365 a         1392 c         1215 g         1405 t
ORIGIN      
        1 caggcttata cctacgcgat tctcatacaa gtcttgccat tttaaagagg gctgttcttc
       61 tttgttgagg gttactttac ccgaaggtat aatatattct ccctggccaa aagcactaag
      121 gccataacca atatttacgc caaactccgc gccaacagga taaaaagtag aggaaattgt
      181 gtttttttct gcattggcgt cttttgtttt ttccgacacg gagtcgcccc cgatgcccgc
      241 atataacccc aatctgaata cgctcagaaa atgtatatcc tttttgagat atagtacgaa
      301 tctgattccc gttccggcca cggggtcact ccctgttcct ctcaatgaat cttgggaata
      361 aataatattc cctcccaatt taattcctgt aggaaaatta ctatttcctt tggctatcag
      421 caatgaaaga ttcccttcaa cgccaccgcc ccaatcatcc atctgattgc caaacggtat
      481 ctgcggaatg ccgtgtatta cctgaaacgc gatcccttta tctttatact cttttatcag
      541 catgccttct tttataaaac caacccctcc tattctgaaa aacttgctgt aagcaccgtt
      601 tcccgtggca atcctattgg aggcccttac cagtgctatc cgcgtctttt ttatttcgcc
      661 tgatgcggtc tctctttctt cataaatggc gtatcttttt tctggataaa ggccatcgcg
      721 tgcgccgatg tctgctaata tagggttgtt atttgcaacg cgattactaa ggatgccgcg
      781 gcctactatt ttatcaaaca aattatttac acctgtcttg gcccaccctt ttagtaagtc
      841 tgcatctgaa agtttgctgt cattgatgga agcggcgctt tgtatgattg gattaagtcc
      901 ctttttaatg tcctggggtc gataaacctt aaccggaatc aactcaaatt tatattcgtt
      961 atactgtttg gctttagctg ctaaaacaga cggagtatcc tcatccaaag gccacatatt
     1021 ataaaaatcg ttaacagttt ggtcatcata tttcagtttg aagacataag ccgtcaggtg
     1081 ccccataaag cccctcatgc tcctctcgac actcgcgctt gaacctgatt tcctatattc
     1141 cctttccaaa ttatcataat gctctctaaa tgaaatcaag tctgtagggc gcaaaacgac
     1201 aatatatgaa ttaggaataa gattcaaacc ccggtcttgt aattcggcaa taccccgtgg
     1261 atcatgaatc gtatcaagaa tactttttat tcgagtatta aatacacccc gtgcagcgat
     1321 cgtgtccaat ttgaatcgcc catttgactg acgctgccac caaaaagaca ttatctctcg
     1381 cccaacccct ttggatgtta gctcttctgc cattctgtca atgtcaaagt ttgaaggaga
     1441 aaaatatttg gtggaaattg tattccagtt aaatcgccta atgtattccc cttctaattc
     1501 agattgaaat aaaggaccta cccctttgga ttcaggcaca tagataatgg tgtaactatc
     1561 ccgatctgta ttggactcct gtgaaaacag atcagcaaat gccgtggtga cgagcgcaaa
     1621 caacagcaaa aaaatgtagt gctttttcat acctgtttaa attgagttgg taaatggttg
     1681 aagatgattt ttgaaaattt tttccaataa ggtttatcaa aggttttata cccgatgaaa
     1741 aatgatttgc caaaaaatat aaaccatttt tcatcgggat aactgatagc cagtggtttg
     1801 caaaannnnn nnnnnnnnnn ctaaaattcg caaaccactg gctatcgagt atagatgatt
     1861 gatgaccata gcgctcggcg gtataaaaca gaattaaaaa tactgacttc aactccgcgc
     1921 aagacgcagt gccccaaaat atgagcagca gtgttttaaa aatctcgttc gcctccccca
     1981 cccccgcaaa ataccctgcc cttcacatta cccccggtag caccccacta cctttgtagc
     2041 accctgctac ctattttttt gtcgggcgaa tgacactgcc ttacaccaat ccctgttttt
     2101 tgaggaagcc caccacatcg gcggcctggg taaaagcgtt gagcggaaac aaatcccggc
     2161 ccttttgcag tacgccgagg cggtgcttat ccaccgttcg gtcgctgata cgcatggtga
     2221 ttgcgatgtc tttggccgaa gcgccctgcg ccatgagttg tatcagttcc tcctggcggc
     2281 gcgagaagcc caagttttcc agaatacccg atttgatttt gttgagcgcc tgctgaagcc
     2341 gttgagcgtc tgccggaaag cgcggatcgg gatacaccgc cgtctcgaaa ggctctccct
     2401 gataaggccc cagtatcgtg taatagctga tataggccgc caggcgcccc tgctcgtcaa
     2461 accccaccgg cgtcgatagc tgatacactc gccagtattg cccggcgtgg tcgcgcagcc
     2521 cgcgtgtgtt gcaatagaac acctttcggt ttttgagcag ttcgggccgc gccgacaatg
     2581 ttttcagcac attatgagtg tgtacggagg ccagatggcg ctggttttcg tgtgttatcc
     2641 ttcttatatc gtccaagccg ggcagccagc cgggggcata ccccaatatc tccatgccgc
     2701 aagactgtat ggcgcgtgcc tcggctacat ccatcagcaa cagcatcgtg cgcggcggct
     2761 tgtacttgca cagccatttc caatgatcga aataatcctg cacggaaagc agcgcctccg
     2821 ccgattgagg tgcaagggta ggtgtgttgt cgtatatcat tatcgtccta agtgttgtct
     2881 tctttcgtcg cgccgctcac acccagccct tgtcgcgggc gtagagcatc acatccaccg
     2941 atcttttcac ccccagtttg cggcgcatgt ttttgaggtg cgcctctatg gtgcgcggcg
     3001 ccagatcgag tcggcaggca gcgtcgcggg cggtattggt caaggcccac acgcgcagca
     3061 cctgcaattc ccgttcggtg aagcgcagcc gcgaatcgtc gagcacatcg gcaaagaaca
     3121 ggagcgcttc ctctgtagag accgccacat ctgccggcaa ccgcacatca aaagacaccg
     3181 actcaaaccc gggcaggcga cttacgtctg tgcagaagca ttgatagtgc tccaaacggc
     3241 ctgtactgcc catcgcgtcc ggttgcagat agcgcagtat tttggcgtag ctgccgtcgg
     3301 cgcgtttcat gcggtgggcc agtatgaagg tgctgcgcaa ggcagcatcc ggtcccagtt
     3361 ggatggcaaa atctccgatg cgccgaacca agcgggcaat ctttccgcgc tcttcgggat
     3421 gcagcagttc ttcggtaaag ggcggcaacg cttccgccga agggtagccg agctgcgctg
     3481 ccaatccctg tccttgttca atgcgccaat gcagtgcgtt cactgcatat tgaaaatcta
     3541 taaccggggt aaagcccgaa gagcagcccg gcatatacgg cccggaactc gtcgttttgg
     3601 gggagatagc agggcctcga tgcttttccg aatagttagt catagtttaa tcattttggt
     3661 acccctgttt ttacggatat atttttctac tacgcatccc aaaggtaaat tcagaacagg
     3721 ggctggagca agctacccga actgtaaaaa ataggctgaa aagccatttt tttaagagcg
     3781 aaatttattt acaataaact cctcaacaat gctttatatc actgcgttgt atagcctgaa
     3841 aatgaggcaa aaaaatctgc gtcacctaaa aaaaacaact tcgccatgca cggcagtacc
     3901 ttgatcggcc tactgaaaaa tctggatgaa aagagttttc gcgctttccg ggattttgtc
     3961 cattcgccat atttcaacaa cagaagtaac gtagtacaac tcttcgagta catcaaaccc
     4021 tgctaccctg cttttgagga cgccaaattg gagcggttca tggcactgag aagactttat
     4081 ccacccggca cttccatgca aaggctgaac gacgacatgt cgaaactgag caaattgtgc
     4141 cggaaatttt tggcgcacaa cgaactggaa gaaagccctc tattagaaaa aaaacttttg
     4201 ttggacgctt accgtcgccg gggaatgacg gagaagcttt cggaagacac ggagcgcttt
     4261 gtccaacaac ttcgagacga acctcatacg gatatcctgc cgctgcgact gagtatggag
     4321 gcgcaatttg aggcgttttt ttatccgcct accgaaaaat acaccccgtt cgcgccgtat
     4381 ctatatggcg ccttacatgg cctggagcag tattatcagt tgcataaagc ccgtattgtg
     4441 gcggaaatcc ttcatcgccg gcatatttat caggaggcgg aaattcctgc gccggccgcc
     4501 cccgccgtat tggatatgta ccggcggttg ctggatattt tggagtcgcc taagccacat
     4561 tccggctttg aaccgctgtt ggacgatata gagcgccact ttgcttcact ggccgacttt
     4621 gaccaacgaa tgctgctcga ccgtctgtat catgttgcca atcggcgtta tgaaaaaggc
     4681 caaccggaat atgtacccat cctttttcgc atcatcaaaa tagccgatca atacgaagcg
     4741 ctcacttacc aaaacgccat tacgccggta gctttggtaa actatgctgc cgtagcaggc
     4801 atggcagggg atttggaatg ggggcggcag tttgtgacgc gctgtcagcc gcaactcacc
     4861 ccagagcata gagaggccgc catccatctg gcggaggcgc acttgctttt ttatgaaggg
     4921 caatttagtc catgtttact tcacatcggc gaggcagagc gctaccatgc agggatgcgc
     4981 attcatgccg aaacgctgta cttgcgcacc tattgcgaac tcgaaaggca aaagggcgaa
     5041 agctatttag aggaatttca acgctgccaa aagcgatttt caaaattcct gcaccatgat
     5101 gcccgcatca ataaagaaaa gataaaagcc ctgcgcaggt tcatcctgct gagttcctgc
     5161 ctgatgcaat atcacaacag gcggaatgaa aaacagcgcc aaaaactgca tcagcagcgc
     5221 attcacaagt acttcaacgg cgcggataaa gtatattctg ctttttggtt gcaaaatcac
     5281 atccgagcgt ttgtgctccc ctgttgattg ggcagaaggt cgtacgcccc ctctgcctgc
     5341 tctacacgtt ccgctacccg gtggtatcgt ccgagccaat gagtagctca at
//
LOCUS       DAORLF010000337         5373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8343_length_5373_cov_1.914442, whole genome shotgun sequence.
ACCESSION   DAORLF010000337 DAORLF010000000
VERSION     DAORLF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8343_length_5373_cov_1.914442"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>242
                     /locus_tag="PK198_20675"
     CDS             <1..>242
                     /locus_tag="PK198_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41225.1"
                     /translation="PSFWSADQSNRFLWQQLKWQQRITDVTARYTLATKRLIAEIEPI
                     YTRTASRFQNVISLSEAPGLPAISVLPDVGTPAIQS"
     assembly_gap    243..394
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <395..>650
                     /locus_tag="PK198_20680"
     CDS             <395..>650
                     /locus_tag="PK198_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41226.1"
                     /translation="KFKKDAELRAQYAWQQGLSPDEYFITRPFLAESYQIQQPFALLE
                     NPSGHAITLHYGKRNAFTFRSWFVNLRYQFGQQWWRSSDNF"
     assembly_gap    651..800
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <801..1265
                     /locus_tag="PK198_20685"
     CDS             <801..1265
                     /locus_tag="PK198_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41227.1"
                     /translation="TAEKESFLLTHSLGRARAEASIALPAHFRLILDNEWSLSSTFRP
                     GFPAAPGNRIHNNRSAITGMYRHGGWQLSASWHYSVFDNQAGGSARLSGWQMVAQKEV
                     KWRQKPLTFGLRAVNLGNAAEFSVIRSSDYFLLKDSVKATAPFMILYVDYSF"
     gene            1293..1952
                     /locus_tag="PK198_20690"
     CDS             1293..1952
                     /locus_tag="PK198_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41228.1"
                     /translation="MKKCLLWLFGLVIVFGNPTVSAQNATYQGIVKYIPADKYEVSPD
                     TIIVYFGKNKMKTVRTGVFIERFGGVVEEITDFEKPERQTTVVYSPHYGKSPDTLTST
                     KSGIVSVKHHPDSVKTILGFACTKVDLIFEPSEYGGGVHTLTVSHWNTSSACLHYAIP
                     EAARFSQFHSAYSGGGIPLSIVSSVHSKMDSGEERIKSSALIACELTVKQLYDSVFQI
                     P"
     gene            complement(2031..4757)
                     /locus_tag="PK198_20695"
     CDS             complement(2031..4757)
                     /locus_tag="PK198_20695"
                     /inference="COORDINATES: protein motif:HMM:NF036565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family peptidase N-terminal accessory
                     domain-containing protein"
                     /protein_id="HRF41229.1"
                     /translation="MKKTLLITAFLMLCGGYGFAQCGMNEVEIKVEIITDPYGSETYW
                     TLTNLMGAVVMQGGQGGVYGNNTTYKDSLCVPADGCFFFEIYDTYGDGIFAPGGYKLY
                     VNEVLVSSGSDDINRYAYVTAHCPAACARVLNALNDLKAHINGTVTLSGNELSLIRNT
                     FVQFPTCLAENESVILLGKTVVEDFDNQIGPLFTTPNTMDGFSKNPANAPGLETERAM
                     LALQQGIFDAVFTPAVYAAYPQHINGWKFNACSVFPGFVAPPSDSSVHKAALIRANFE
                     DPDGMNPYYDINFERMRHALRPTGLYLSPGSIANVTVPDSLVGQDYWIRVGSHDWDLT
                     ERPQYRRLDRISKKFPIDSTTVAVYNPMGGAISILVPYGADDDIVEVSVSNGVEAPFF
                     SLKTFYETTDFEAELSKPGPWAVFESDNVMYTIPKHSIVPGQYDLRQTLQDWETAVRG
                     VNSILARQIVPDKHNMYMITDLDIRTGAYSIGYPMSNTPLNYTDVPGPAYFINGPGSD
                     DEVNFHEAGHALAISKFEGEEEALVNFPYIMAMNYGLNQDLNEAVNYSFVPNTFDIDK
                     TATHRMVSNTFGTNRNISNTVNDEVRYQHRGYGHYFEIVNTLGWCPLRNFWKQESIDF
                     ENGVDYGIHQDNDGRIIRMSVAAQADLRPLFHVFGILPQDSLAVQDSLTQSGVLPSIA
                     IYNRLQAYFDLIPEDNAAFVNYALSVYPNLYTDGPTDAPEYGVGWHYLKSLTYNAAEA
                     QARTAILQSIINQYFPNGAPTGNERPDVCCLLDTMYVNLVNEEVFVTGGVRPYNISID
                     ITQNVKTITVVDYDGCVSTSQYIINSLSEEGNKSISIYPNPASTTLYIDLPDNSNSIE
                     RLLLISINGQVLKEYRKPDQFIDISTLSDGVFILRIELAGGEQINKKVVILK"
     gene            complement(4954..>5373)
                     /locus_tag="PK198_20700"
     CDS             complement(4954..>5373)
                     /locus_tag="PK198_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41230.1"
                     /translation="KCYKEKFSRIHFDSFDRNSVRIPDRDFYYNITYESIFYAVKECN
                     AKRIGISHLSASGNYHQDIAMCTAEALAHFHDAHPGKIENFAFVGCCITENHLRGTQR
                     LNNERNTRHIEIGKQISVDNNIQIINLDIRRKEWENV"
BASE COUNT         1293 a         1218 c         1181 g         1379 t
ORIGIN      
        1 cccctcattt tggagcgccg atcagagcaa tcgtttcctt tggcagcaat tgaaatggca
       61 gcagcgcatc acggatgtca cggctcgata taccttggcc acaaagcgtc ttattgccga
      121 aatagagccg atttataccc gtaccgcttc gaggtttcaa aatgtgattt cgctctcgga
      181 ggcccccgga ttgccggcca tttccgtttt gcccgatgtg ggtacgccgg ccattcagtc
      241 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaagtt caaaaaagac gcagaactta
      421 gagcgcagta cgcctggcag caggggctgt cgccagatga gtatttcatt acccggcctt
      481 ttctggcgga gagttatcag atacaacagc cttttgcact gctcgaaaac ccttccggcc
      541 acgccattac gctgcactac ggaaaacgca acgccttcac ctttcgctcc tggtttgtca
      601 acctccgata ccagttcggg cagcaatggt ggcgcagttc cgacaatttt nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn accgccgaga aagagagctt cctgcttacc cactcgctgg
      841 ggcgcgcaag agccgaggcg tctattgctt tgcctgcgca tttcagactg atattggaca
      901 atgaatggtc gctgagcagc acattcaggc cgggttttcc cgctgcaccg ggcaatcgca
      961 tacacaacaa ccgctcggcc atcacaggca tgtaccgtca tggcggctgg caactgagcg
     1021 catcctggca ttattccgtt ttcgacaatc aggcgggcgg ctcggcccgt ttgtcgggtt
     1081 ggcaaatggt ggcacagaaa gaggtgaaat ggcggcaaaa accgctgact tttggtcttc
     1141 gggcagtcaa cctcggcaac gcagctgaat tttccgtcat tcgcagcagc gattatttcc
     1201 tgctcaaaga ctccgtaaaa gcaacagcgc cgtttatgat cttgtatgta gattactctt
     1261 tttgagaatg gcacacgaca aaaaacatcc acatgaaaaa atgccttctt tggctattcg
     1321 ggcttgtcat cgtattcggc aatccgacgg tatcagccca aaacgctacc tatcagggaa
     1381 ttgtcaaata cattcccgcc gataaatacg aagtatcgcc ggacaccatc atcgtctatt
     1441 ttggaaaaaa caaaatgaaa accgtaagaa ccggggtgtt tatcgaaaga ttcggcggcg
     1501 ttgttgagga aataaccgac tttgaaaaac ctgagcgcca aaccaccgta gtatatagcc
     1561 ctcattatgg aaaaagcccg gatacattga cgagcacaaa aagtggaatt gtctccgtga
     1621 aacaccatcc cgactcggtc aaaacgatcc tgggcttcgc ttgcaccaag gttgatctca
     1681 tctttgaacc gtcggagtac ggcggcggcg ttcacaccct gacggtgtct cattggaaca
     1741 cctcaagcgc ttgcttacac tacgcgatcc ctgaagcagc ccggttctct cagttccaca
     1801 gcgcgtattc cgggggagga atcccattgt ccatcgttag cagtgtgcac tcaaaaatgg
     1861 acagtggcga agaaagaatc aaatcaagcg ccctgattgc ttgcgagttg acggtgaagc
     1921 agttatacga ttctgttttt caaataccct gaaagatggc atcctatttg ccctgttggt
     1981 tttttcgtat aggaacgatg tgttgatgcg ccggttttta tgcgctgtcc ctacttcaaa
     2041 atcacaacct ttttgttgat ttgttcaccg ccggccaatt caattctcag aataaacaca
     2101 ccgtcactta gtgtggatat atctatgaat tggtcgggtt ttcgatactc ttttaatact
     2161 tgcccattga tggaaatcaa tagtaagcgc tctatggagt tgctgttatc cggcaaatca
     2221 atatagagtg ttgtcgaagc aggatttgga tatatgctta tgcttttgtt gccttcttca
     2281 gacaggctat tgatgatgta ttgactagtg gatacgcaac catcataatc caccaccgtt
     2341 atggtcttga cattttgggt tatgtcaata gaaatattat atggcctcac gccgcctgtt
     2401 acgaagacct cttcatttac aaggttgaca tacatggtgt ctaacaggca gcagacatcc
     2461 ggcctttcat tgcccgttgg agcgccattg ggaaaatatt ggttgatgat ggattggagg
     2521 atggccgtac gggcctgggc ttcggctgcg ttgtacgtca acgatttcag gtaatgccat
     2581 cccaccccgt attcaggagc atctgtcggg ccatccgtgt ataaattggg ataaacagac
     2641 aaggcataat tgacgaacgc tgcattgtct tccggaatca ggtcaaaata ggcctgcaac
     2701 ctattgtaga tggcgatgga aggcagcacg cctgattgag tcaaactgtc ttgtaccgca
     2761 agggaatctt gtggaagaat gccaaaaaca tgaaacaatg ggcgcaggtc tgcctgtgcg
     2821 gcaacggaca ttcggatgat cctgccgtca ttgtcttgat ggatgccgta gtcaaccccg
     2881 ttttcaaagt caatggattc ctgtttccaa aaattgcgga gcgggcacca tccgagcgta
     2941 tttacgatct caaaataatg cccgtagcct ctgtgctggt aacgtacttc gtcatttacg
     3001 gtgtttgaaa tatttctatt ggttccaaat gtgttggata ccatccggtg ggtagcggtt
     3061 ttgtcaatgt caaaggtatt ggggacaaaa ctgtaattca ccgcttcatt taagtcctgg
     3121 ttcaggccgt aattcatcgc catgatgtat ggaaaattga ccagggcttc ctcctcgcct
     3181 tcaaatttgg agatcgccag ggcatgtccg gcttcatgaa aattcacctc gtcatcagac
     3241 cccggaccat taatgaaata agccgggccg ggcacatctg tatagttcag cggcgtgttc
     3301 gacattggat aacctattga atatgcgcct gttctgatat ccaaatcagt aatcatatac
     3361 atattgtgct tgtcgggaac aatctgtcgg gccagaatgg aattgactcc ccgtacagcc
     3421 gtctcccagt cttgtaaggt ttgtcggagg tcatattgac cgggaacaat ggaatgtttg
     3481 ggaatggtgt acatcacatt gtccgattca aagaccgccc aggggccggg cttgctcagc
     3541 tctgcctcga aatccgttgt ttcatagaat gttttcaatg agaaaaaggg cgcctcaacg
     3601 ccattgctca cgctgacctc tacaatgtca tcatcggcgc cgtagggtac aagtatcgaa
     3661 atggcgcctc ccatcgggtt gtaaaccgct acggtcgttg aatcaattgg gaatttcttc
     3721 gatatcctgt ccaaccgcct atattgaggt ctttcggtca agtcccaatc atgcgacccc
     3781 accctgatcc aataatcttg gccgaccaag ctatcgggca ccgtaacgtt ggctatactt
     3841 ccgggagaca aatacagccc ggtgggccga agggcatgtc tcattctttc aaaattgatg
     3901 tcgtagtacg ggttcatccc atcagggtct tcaaaatttg cacgtatcag tgccgcttta
     3961 tggacagatg aatcggatgg cggggccaca aaaccgggga aaacgctgca cgcattgaat
     4021 ttccaaccat tgatgtgctg cggatatgct gcataaactg ccggggtgaa tacggcatcg
     4081 aatatcccct gctgaagcgc aagcatggcc cgttccgttt ccagtccggg agcattggcc
     4141 ggatttttag aaaaaccgtc catcgtattg ggtgtagtga aaagtggccc gatttgattg
     4201 tcaaaatctt ccaccaccgt tttgcctaat agaatgacag actcgttttc ggcaagacaa
     4261 gtcgggaact gaacaaaggt gttcctgata aggctcagtt catttccaga aagcgtaacc
     4321 gttccattga tatgagcctt gaggtcattc agcgcattca gcaccctggc gcatgcagcc
     4381 ggacaatgtg ccgtaacata agcatagcga ttgatatcat cggacccgct ggaaacaagc
     4441 acctcattta cataaagttt atacccgccg ggagcaaaaa tgccgtctcc gtaggtatcg
     4501 tatatctcaa agaaaaagca gccatcagca ggaacgcaaa ggctgtcttt gtaagtggta
     4561 ttgttgccgt aaacgccgcc ctggccgccc tgcatcacga ccgcacccat taaattggtc
     4621 aaagtccagt aggtctcact accataaggg tccgtaataa tttctacctt gatttctact
     4681 tcattcatac cgcactgggc aaaaccatag ccgccacaaa gcattagaaa agcagtaatt
     4741 agtaatgttt ttttcatttt gctttgaatt taacggctgc atctgcgctg tttgcggctg
     4801 cgttgggctt gcgggttggc gttgggcagc attttcgtaa agcctttgat gggcggatat
     4861 tatacccaaa ctacagaggt tgtcgaatca gtacatctga aaaccaagga cttggttcgt
     4921 tgatttttcg atacccactt tccgatgagt atattataca ttttcccatt cttttctcct
     4981 gatatcaaga ttgatgattt gaatattatt atcaactgaa atttgtttgc cgatttcgat
     5041 gtgtcttgtg tttctttcat tatttaatct ttgagttcct cgaagatgat tttcggtaat
     5101 acaacagcct acaaaagcaa aattttctat tttacccgga tgcgcgtcgt gaaagtgtgc
     5161 taatgcttcc gcggtacaca ttgcaatatc ttgatgatag ttaccacttg ccgaaagatg
     5221 acttattcca attctctttg cattgcactc tttaactgca taaaaaatgg attcataagt
     5281 tatgttgtag taaaagtctc tgtctggaat tctgacacta tttctatcaa aactatcaaa
     5341 gtggatacga gaaaattttt ctttatagca ttt
//
LOCUS       DAORLF010000338         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8348_length_5372_cov_1.955614, whole genome shotgun sequence.
ACCESSION   DAORLF010000338 DAORLF010000000
VERSION     DAORLF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_5372_cov_1.955614"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK198_20705"
     CDS             <1..261
                     /locus_tag="PK198_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41231.1"
                     /translation="MSKIDDYMKKYSTVEHLLEEAPQCEKDDFLRLRRKVEVSYDENP
                     LGDQHALDAFLRVVSLQEESYTFDDQHPYFSVFAWHFDLYNT"
     assembly_gap    265..385
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <386..708
                     /locus_tag="PK198_20710"
     CDS             <386..708
                     /locus_tag="PK198_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41232.1"
                     /translation="RSLGQNILDEKVDDFLSIGQAINNFNKQVWSRYRIFIMHEGLKS
                     LYESNEHFRVQLNNTSNQSMVYHSSISYWGMGKTSSDQNYWGKLLTRMKLTSMSHPQA
                     SSLR"
     gene            complement(1077..>2111)
                     /locus_tag="PK198_20715"
     CDS             complement(1077..>2111)
                     /locus_tag="PK198_20715"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF41233.1"
                     /translation="RKAYEMYLGEEKAKQPKNKPFFLPPPHIAELALSQDQINAAAAS
                     ADCAIVTIGRIAGEFADRKAADDFYLWQAEQDLLRMVSETFHSKGKKVIVILNVGGAL
                     DVASWRDKADAILLAWLPGQEAGNAIADVLTGKVNPSGKLPTTFLKRYEDDPTAAGFP
                     GKEFGEPVFMGFATVRQAEIEYEEGVYMGYRACDKQNIEPAYEFGYGLSYTTFSYTDL
                     KLSSPKFKNKMTVSVTVTNTGKVAGKEVAQLYLSAPALSMDKPAQELKGFAKTRLLQP
                     GESQVLSFDLDARALASFDEKSAAWLADKGLYTVRVGGSSRGMRLQAEFKLAKSMVVE
                     TVSRLPAMER"
     assembly_gap    2112..2258
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2259..>2596)
                     /locus_tag="PK198_20720"
     CDS             complement(<2259..>2596)
                     /locus_tag="PK198_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HRF41234.1"
                     /translation="KDWGFKGFVMTDWFGGDNAVEQMKAGNDLLMPGTPKQVELITQA
                     VKEGRLEERFLDENLATMLPIFRQSPAFKGYPASLSPDLKSHAVVARQAATEGMVLLK
                     NDVGTLPLRN"
     assembly_gap    2597..2639
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2640..3402)
                     /locus_tag="PK198_20725"
     CDS             complement(<2640..3402)
                     /locus_tag="PK198_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF41235.1"
                     /translation="MKKTILLWLISLVMLGPVFGQTAPTLGKNSDQEVISTMSLDEKV
                     RLLVGMGMRLPGMPGNAQQGPVVGQTQDKVPGAAGTTATFQHLGIPGLVVADGPAGLR
                     ISPTRPGEDKTYYCTAFPIATLLAASWDTPLVGKVGEAMGRETKEYGVDIILGPGMNL
                     HRNPLGGRNFEYYSEDPLLTGKMAAAMVNGIQSNGVGTSIKHFAVNNHETNRNTINVK
                     VSQRALRELYLRGFEIAVKESNPWTVMSSYNKVNGT"
     gene            complement(3537..>5372)
                     /locus_tag="PK198_20730"
     CDS             complement(3537..>5372)
                     /locus_tag="PK198_20730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRF41236.1"
                     /translation="FFFLFVLYLFSPTPPSAPAFRFAIVSDTHIGGAPTASEDLRRTV
                     ADINADSTLAFVVISGDVTELGSDVELAEAHEILRHLNKPWYVIPGNHDTKWSESGNN
                     SFLQVFGYERFYFEYGGFVFLGCNSGPNMRMAPGLVPAEDLRWLDSIVVQLPAGQPLV
                     FFNHYPLDEGLGNWYKVTDILKKANTQAAFCGHGHANKILSAEGIPAAMCRSNLRAKA
                     DTGAYNIVAVFPDSLTLSERRPGGRTTAPWHRIALGQRDFSADTTRYSRPDFSINERY
                     PQVRTLWQIQDPCDIGAGIAVSGDVAVYANTCGNVKAVFWKNGTPKWAFSALGKIYST
                     PAIEKGRVVFASADSAIYCISVYSGELIWQFKTGKAIVASPVIADGVVYIGSSEGKFR
                     AISLQSGALLWEFGGVEGFVETRPAVDAERVYFGSWGTYFYALNRKDGALAWRWSNGG
                     SNRMLSPAAVFPVLADGKVFIVAPDRFATALDARTGRVVWRTDKKVGRESIGISADQQ
                     TIYVKSMTDTITAFSAKGSTLQQKWQAPAGYGYDIAPSPMTERAGRLFIPTDKGVLIA
                     LNPATQKVEWSHRLCNALINNAVPLPDGRVLGTTMDGLVFLLQEK"
BASE COUNT         1159 a         1360 c         1322 g         1220 t
ORIGIN      
        1 atgtcaaaga ttgatgacta tatgaaaaag tattctactg tagagcacct tctggaggag
       61 gctccacagt gtgagaaaga tgatttcctt cgattaaggc ggaaggtaga ggttagttat
      121 gatgagaacc cattgggtga ccagcatgct ctcgatgctt ttttacgagt ggtctcattg
      181 caggaggaga gctacacttt tgatgatcag catccatatt tttcagtttt tgcatggcat
      241 tttgaccttt acaacacata acaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnacagg agccttggac agaatatctt ggatgaaaaa
      421 gtggatgatt ttttatcaat aggtcaagct attaataatt ttaataaaca ggtatggagt
      481 aggtatcgaa tcttcataat gcatgagggg ttaaaaagtc tttacgaaag taatgagcac
      541 tttcgcgtgc agctaaacaa cacctcgaat caatcgatgg tttatcattc atccatatct
      601 tattggggta tgggcaaaac ctcttcagat cagaattatt gggggaagtt acttactcga
      661 atgaagctga catcaatgag ccacccgcag gcatcgagtc ttaggtaaca tagttacagc
      721 ccaaagataa aatcacccta tacaccctgc cccgctggcg cagagtcttc tctcattcaa
      781 aaaaaataaa aaaaatcaat cgctggtggc gtggggcgca atgaggcgtc cccgcacccc
      841 tatttcgttg cttctgacaa catactgaaa aacaaatctt tatgccccac ccttcaccca
      901 agcccctgct ccccaagtat ccgcgccacc tcctcctcgc tgatggcggc aatcagcgca
      961 ataaccggaa tggcgatgcc ctgcttgtag gcattgacga caaggtacag acgctcttct
     1021 tcgcggcctt tttcaattcc ttgtattgca agtgttttgc attatggcca ttccactcac
     1081 cgctccatcg ccggcaaccg gctcaccgtt tccactacca tggattttgc caatttgaac
     1141 tcggcctgca agcgcattcc ccgactggag ccgcctaccc tgacggtgta aagtcctttg
     1201 tcggcgagcc aggcagcgct tttttcatca aaagaagcca gggcgcgcgc atccagatca
     1261 aaactcagca cctgcgattc gccgggctgg agcaggcgcg ttttggcaaa tcctttcagc
     1321 tcctgtgccg gcttatccat gctcaatgcc ggagcgctca ggtacagttg ggccacttct
     1381 ttgccggcca ctttccctgt gttggtaacc gttaccgata ccgtcatttt gtttttgaat
     1441 ttcgggctgc tcaacttcaa atcggtgtag gaaaatgtag tgtaagaaag gccgtagccg
     1501 aattcgtacg ccggttcgat gttttgctta tcgcaagccc ggtagcccat gtaaacgccc
     1561 tcctcgtatt caatttcggc ctgccggacg gtggcaaatc ccataaaaac aggctcgccg
     1621 aattcctttc cgggaaaacc ggccgctgta ggatcgtctt cgtaccgctt cagaaaggtc
     1681 gtcggtagtt tccccgatgg attgactttc cctgtgagta catcggctat ggcgtttccg
     1741 gcttcctgtc ctggcagcca agccagcaga atggcgtcgg ctttatcgcg ccaggaagct
     1801 acatccaagg ccccgccgac attgaggatg acgatcactt ttttgccctt gctgtgaaaa
     1861 gtctcggaaa ccatgcgtaa caaatcctgc tcggcctgcc acaaataaaa atcatccgca
     1921 gccttgcgat ctgcaaactc tcctgcaatg cgcccgatgg tgacaatggc gcaatcagca
     1981 cttgcggcag cggcattgat ctgatcttgc gacagcgcca gttcggcaat atgcggcggc
     2041 ggaaggaaaa atggtttgtt tttgggctgt ttggcctttt cttcgccgag gtacatctcg
     2101 taagctttgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ttgcgcaatg gcaaggttcc
     2281 aacgtcattt ttgaggagta ccatgccctc cgtcgctgcc tgacgcgcca caactgcatg
     2341 gcttttcaaa tcaggcgata gggaggcggg ataacctttg aaggccggcg attggcgaaa
     2401 gataggcagc atagtcgcca gattttcgtc gagaaagcgt tcttccaggc ggccttcttt
     2461 cacagcctgg gtaatgagtt ccacctgttt gggcgttccg ggcatgagca gatcattgcc
     2521 ggctttcatc tgttccacgg cgttatctcc gccaaaccag tcggtcataa caaagccttt
     2581 gaaaccccag tctttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2641 agtgccgttg accttgttgt agctgctcat taccgtccag gggttcgatt cttttaccgc
     2701 aatttcaaaa ccccggaggt acagttcgcg cagcgcccgt tgactcactt ttacattgat
     2761 ggtgttgcgg ttggtctcgt gattgttgac tgcaaagtgt ttaatggagg tgcctacgcc
     2821 attcgattgt atgccattga ccattgcggc tgccattttg cccgtcagca gggggtcttc
     2881 agagtagtac tcgaaattgc gcccgcccaa gggattgcgg tggaggttca tacccggacc
     2941 gagaatgatg tccacgccgt actctttggt ttcgcggccc atcgcttcgc ctacctttcc
     3001 taccaagggc gtatcccagg aggccgccag cagtgtagca atgggaaatg cggtgcagta
     3061 atacgttttg tcttcgccgg gccgggttgg cgaaatgcgc aatcccgcag gcccgtcggc
     3121 gactaccaat cccggaatgc ccaagtgttg gaaggtggcg gtggtaccgg cagctccggg
     3181 tactttgtcc tgcgtttggc ccaccacagg gccttgttgc gcattgcccg gcattcccgg
     3241 caaacgcatg cccatgccga ccagcagacg cactttttca tccaacgaca tggtggaaat
     3301 gacttcctga tcgctgtttt ttcccaatgt cggcgctgtt tgtccaaaga caggccccag
     3361 cataaccaag ctgatgagcc ataataaaat ggtctttttc atattgtttg tgttgattgt
     3421 atctgtaaaa atcacttttc ctgtaataaa acgctcgcgc gagattccat ctcgtgtgag
     3481 ctattctgcg ggttctaccc gcatgcaata ttcctactcg aagggatcat aataaatcac
     3541 ttttcctgta ataaaaacac cagcccgtcc atcgtagtac ccaacactct gccatcgggc
     3601 aagggcacag cattgttaat caaggcattg cataacctgt gcgaccactc cactttttgc
     3661 gtggccgggt tgagggctat cagcacgcct ttatcggtgg gaataaacag ccgaccggcc
     3721 cgctccgtca tgggcgaggg cgcaatgtcg tagccgtagc cggcgggtgc ctgccatttt
     3781 tgttgcaagg tgctgccttt tgcagaaaaa gccgtgatgg tgtcggtcat ggatttgaca
     3841 taaatcgtct gctgatcggc tgagattccg atggactcac gacctacttt tttgtctgtc
     3901 cgccaaacaa cgcggcccgt tcgggcatcc aatgccgttg caaaacgatc cggcgccacg
     3961 atgaacacct tcccatctgc caacaccgga aaaaccgccg ccggcgacaa catgcggttg
     4021 ctccctccat tgctccagcg ccaggcaagg gcgccgtctt tgcggttcaa ggcataaaaa
     4081 taagtgcccc aactaccaaa atacacccgc tcggcatcca ccgccggacg agtttccaca
     4141 aagccctcta cgccgccgaa ctcccacaac agcgctcctg attgcaaaga aatcgcccta
     4201 aacttgccct ccgaggagcc gatatacacc accccgtcag cgatgaccgg cgaggctacg
     4261 atggctttcc ctgttttgaa ttgccagatc aattcacccg aatatactga aatgcaatag
     4321 attgcgctgt ctgcggaggc gaacactacc ctaccctttt cgatggccgg cgtggaatag
     4381 attttgccca gcgcggaaaa cgcccatttc ggggttccgt ttttccaaaa aacagctttc
     4441 acattgccgc aggtgttggc atacaccgct acgtcgccgg aaacggcaat gcccgcaccg
     4501 atgtcgcagg ggtcttgtat ttgccacagg gtcctgacct gcgggtatcg ctcgtttatg
     4561 ctgaaatcgg ggcgggaata gcgggtcgtg tcggcgctga aatcccgctg ccccaaggcg
     4621 atgcgatgcc agggcgcagt cgtgcggcct ccgggtcggc gctcggaaag ggtgagcgaa
     4681 tcgggaaaaa cggcaacgat gttgtaagcg ccggtatctg ctttggcgcg gagattggaa
     4741 cggcacatgg ccgcaggaat gccctctgcc gacaggattt tattggcgtg gccatgaccg
     4801 caaaacgccg cctgggtatt ggcttttttc aggatgtctg tcactttgta ccaattgccc
     4861 aaaccctcgt ccaaagggta gtgattgaaa aacacaagcg gctgaccggc aggcaattgt
     4921 accacgatgg aatcgagcca gcgcaggtct tcggcgggta cgaggccggg cgccatgcgc
     4981 atgttggggc cgctgttgca acccagaaaa acaaagccgc cgtactcgaa ataaaagcgt
     5041 tcgtagccga aaacctgcag aaagctgttg ttgccgcttt ccgaccactt ggtgtcgtgg
     5101 ttgccgggaa tgacgtacca gggcttgttg aggtgacgga gtatctcgtg cgcttcggcc
     5161 aattccacat cggagccgag ttcggtcacg tcgccggaaa tgacgacaaa ggccagcgtg
     5221 ctgtcggcat tgatgtcggc aacggtgcgg cggaggtctt cggaggccgt gggcgctccg
     5281 ccgatgtggg tgtcggaaac gatggcgaaa cggaaggcgg gcgcggaggg cggcgttgga
     5341 gagaaaaggt aaagaacgaa gagaaagaag aa
//
LOCUS       DAORLF010000339         5361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8374_length_5361_cov_1.883717, whole genome shotgun sequence.
ACCESSION   DAORLF010000339 DAORLF010000000
VERSION     DAORLF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_5361_cov_1.883717"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>254
                     /locus_tag="PK198_20735"
     CDS             <1..>254
                     /locus_tag="PK198_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41237.1"
                     /translation="EPSLALRMPAAEPMGDSKPAWWMAKQLAERLGLGAYFPYKDFSE
                     VLDWQLKQIGSSLEEMKKTGVIRLPRKSDNLYIKEGEPMS"
     assembly_gap    255..379
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            397..>689
                     /locus_tag="PK198_20740"
     CDS             397..>689
                     /locus_tag="PK198_20740"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRF41238.1"
                     /translation="MHTFSRTANNPNLTDLMSENDLWVNPKVARIWGLENGQYVWLKN
                     QDGMVSDFSIKVRITERIRWDSVYLVHGFGHNRKELSRAFGKGVSDTQMITAVR"
     assembly_gap    690..801
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <802..1325
                     /locus_tag="PK198_20745"
     CDS             <802..1325
                     /locus_tag="PK198_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF41239.1"
                     /translation="MAIDTKKCVGCSDCVVACQLENNVPSGYCRDWVTETVHGSYPNI
                     EMELRSERCNHCDNAPCVRCCPTGASHIVEGGVVLVTPNMCIGCGACIESCPYDARFS
                     HPDGHVDKCTFCIHRVRKGLQPACVSVCPTKCMYFGDLDDPSSEVSKMLRTRQYKVLA
                     PEAGTKPQVYYLV"
     gene            1421..>1773
                     /gene="nrfD"
                     /locus_tag="PK198_20750"
     CDS             1421..>1773
                     /gene="nrfD"
                     /locus_tag="PK198_20750"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF41240.1"
                     /translation="MKEELIISGRNIPYIDPHLNIWHWPIPAYLFLGGLAAGLLFFAG
                     LYTLMGKEKQLPTAVKWATFVAPFALVLGLFFLFIDLKHQWYFWRLYLTFRIESPMSW
                     GAWVLMAITPLSFVWA"
     assembly_gap    1774..1857
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..>2208
                     /gene="nrfD"
                     /locus_tag="PK198_20755"
     CDS             <1858..>2208
                     /gene="nrfD"
                     /locus_tag="PK198_20755"
                     /inference="COORDINATES: protein motif:HMM:NF025940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF41241.1"
                     /translation="NRRYFASIMVFLALVLGIYTGILLSAFNARPLWNTALLGPLFLV
                     SGMSTGAAVILWMSKDAQERHLFARIDLVLIAIELFFIIHLFMGFMAGPEVQQDAAQL
                     FLGGRYTWAFWGLV"
     assembly_gap    2209..2234
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2493..3167
                     /locus_tag="PK198_20760"
     CDS             2493..3167
                     /locus_tag="PK198_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF41242.1"
                     /translation="MTLLKATAIPLLLLLIGTGCRRSDPSLVQGPEARPAPAQVLLEL
                     NDQRRFMSVDEVADQIIKGLPDLLLVDVRSDAEFQAYSLPGAVHIPLQALLEPAHQQR
                     LDCSKFRIVFYSNDQASAEKAWFITRQRGCRDVYVMQGGLNEWTVAFLQPQEPAQTAP
                     QEVWDQYQFRLAARQYFVGASKALTPEPFQQPAAAPAATDSQTRKNIEVKPKAAKPAA
                     EEEEGC"
     gene            3285..3911
                     /locus_tag="PK198_20765"
     CDS             3285..3911
                     /locus_tag="PK198_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF41243.1"
                     /translation="MNNRRISFLLIAAMAWFAVACGPPSHFERNAQELLNSISEVENF
                     VMVQEAEAAIQQKKTDFVLVDLRAPIEFERGHLEGAVSIPAQHLLESKSLKVFDNKKA
                     TIVLYGRRQEQAHGPWLLLRQLGYDHVKLLQAGYDGIAAADSLSFLAPETALFDYAAS
                     FRQATTEGQKAIEAVKIVPTAVAPAAPKTIITKPKAAAKPAAEEEEGC"
     gene            4340..5233
                     /locus_tag="PK198_20770"
     CDS             4340..5233
                     /locus_tag="PK198_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRF41244.1"
                     /translation="MMIATESTEIATQDSDQPLRRVAIQGIPGAFHEVAARLYFDTAP
                     LEIVPALTFEDLVRRLEQNDGVDIALMAIENTLAGSLMSNYRLLDNSQLSITGELHLR
                     ITQNLMALPGQSIHDIQEVYSHPIAIAQCRDFFRAYPHIRLIEAEDTALSAKQISDRN
                     QSEAAAIAGALAAEMYGLEIIAPGIETNKKNYTRFLVLERRADATEIVDADKVSISFS
                     VDHEVGSLHKVLAVLAAYNVNLSKIQSAPIIGKPWEYLFFVDFVTEGKVGWKQALEAI
                     QPLAHGLKLHGAYRQGKHYDD"
BASE COUNT         1180 a         1464 c         1290 g         1080 t
ORIGIN      
        1 gagccgtcct tagctctgcg catgccggcc gccgagccga tgggcgattc caaacccgcc
       61 tggtggatgg ccaaacaatt ggccgaaagg ctgggcttgg gcgcatattt cccttacaag
      121 gacttcagcg aagtgctcga ctggcaactg aaacagatcg gttcttcttt ggaagaaatg
      181 aaaaaaaccg gcgtcatcag gttgcccaga aagagtgaca acctgtacat caaagagggc
      241 gagccgatga gcttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnna ctacgggcgc gcgcccatgc acaccttcag ccgcactgcc
      421 aacaatccca acctcaccga cctgatgagc gaaaacgacc tctgggtcaa tcccaaagtg
      481 gcgcgtatct ggggcttgga aaacggccag tatgtgtggc tcaaaaacca ggacggcatg
      541 gtctccgatt tttccatcaa agttcgcatc accgagcgca tccgctggga ttcggtctat
      601 ctggtgcacg gcttcggcca caaccgaaaa gaactcagcc gggctttcgg caagggcgtc
      661 agcgacacgc aaatgattac tgccgtgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn ncaatggcca tagataccaa aaaatgtgtg ggttgcagcg
      841 attgcgtggt ggcctgccaa ttggaaaaca acgtgccatc gggctattgc agagactggg
      901 tcacggaaac cgtgcatggc tcgtatccca acattgaaat ggaactgcgc tcggagcgtt
      961 gcaaccactg cgacaatgcg ccctgtgtgc gttgctgtcc caccggcgcc agtcatattg
     1021 tggaaggcgg cgtggtgctc gtcaccccca atatgtgcat cggctgcggc gcctgcattg
     1081 agtcctgccc gtacgacgcc cgcttctcgc accccgacgg ccatgtggac aaatgcacct
     1141 tctgcatcca ccgggtgcgc aagggtttgc agcccgcctg tgtgtcggtg tgccctacca
     1201 agtgcatgta tttcggcgat ttggacgatc cctccagcga ggtttccaaa atgctccgca
     1261 cccgccaata caaagtcctc gcacccgaag ccggcaccaa accgcaagtg tattatctgg
     1321 tctgaaagta ccggcgatct tcagtcgtca aaatccgaaa tcaggaatcc gaaatccgaa
     1381 atccgaaatc aggaatccga aatccgaaat ccgaatcaac atgaaagaag aactcatcat
     1441 aagcggacgc aacataccat acattgaccc gcacctcaac atctggcact ggcccattcc
     1501 cgcctacctc tttttgggcg gactggcagc cggcctcctc ttctttgcgg ggctttacac
     1561 cctcatgggc aaagaaaaac aactgcccac cgctgtcaaa tgggccactt tcgtagcgcc
     1621 gtttgccttg gtactgggcc tgtttttcct cttcattgac ctcaaacacc agtggtattt
     1681 ctggcgtctg tacctcacct tccgcatcga atcgcccatg agctggggcg cctgggtact
     1741 catggccatc acgccgctgt cctttgtatg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     1861 atcgcaggta ttttgcatcc attatggtct ttttggcctt agttttgggc atttataccg
     1921 gcattttgct ctcggccttc aatgcccggc cgctgtggaa caccgcgctg ctcggcccgc
     1981 tgtttctggt ttccggcatg tccacagggg cagccgtcat actttggatg tcgaaagacg
     2041 cgcaggagcg gcatcttttt gcccgcattg acttagtgct gattgccatc gagttgttct
     2101 tcatcatcca cctgttcatg ggcttcatgg cagggccgga ggtgcagcaa gacgcggcac
     2161 aattgttcct cggcgggcgc tacacctggg cattttgggg tttggtcgnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnngtcgtg ccggccctgc tggagatact caaattgcgc ggcctcaaaa
     2281 ttcctgtggc catacccgcc ctgctcatca tactcggcgg cctcatcttc cgcatcatca
     2341 tggtagaagc cggcgaagct acgcgatatt tgtattaatt ttggggcgtg ccggcgcctc
     2401 tgccaagaac ccattggcgc cgtcgcgtgc tttgcccgct cacgcatccg gcaacatcac
     2461 cgacttgcac cattgttatc aatagtaaaa aaatgacact actcaaagcc accgccattc
     2521 cgctgctcct gctgctcatc ggcacgggct gccgccgctc cgatccctcc ctcgtgcaag
     2581 gccccgaagc ccggcccgcc cccgcccaag tcttgctgga actcaacgac cagcgccgct
     2641 tcatgtcggt tgacgaggtg gccgatcaga tcatcaaagg gctgcccgat ctcctgctcg
     2701 tagatgtgcg cagcgacgcc gagttccaag cctattcact gcccggcgcc gttcatattc
     2761 cattgcaggc actcctcgaa ccggcccatc agcagcgctt ggattgcagc aagttccgca
     2821 tcgtatttta ctccaacgat caggcttccg ccgaaaaggc atggttcatc acccggcagc
     2881 gcggttgccg cgatgtgtat gtcatgcagg gcggcctcaa tgaatggacg gtagcgtttt
     2941 tgcaaccgca ggaaccggca caaaccgcgc cacaggaagt ctgggatcag tatcagttcc
     3001 ggctcgccgc acggcagtat ttcgtagggg ccagcaaagc cctcacaccg gagccattcc
     3061 aacagcctgc cgccgctcct gccgccaccg attcccaaac gcgcaaaaac atagaggtga
     3121 aacccaaagc cgccaagcct gccgcagagg aagaagaggg gtgttgaccg gtatgcggtg
     3181 gcgcgctatg gcgcggggtt tccggtgcaa ctcgcggttt gcaactcgta gctcacggct
     3241 cgcagctaat aaataatcat caaaaaaaac actcatcaaa aaacatgaac aaccgacgca
     3301 tctccttcct tctcatcgcc gcaatggcat ggtttgcagt agcttgtggc ccgccgtctc
     3361 acttcgagcg caacgcgcag gaactgctca acagcatatc cgaggtcgaa aattttgtca
     3421 tggttcagga agcggaagcc gccattcaac aaaaaaaaac cgactttgtg ctggtggacc
     3481 tgcgcgcacc catcgagttt gagcgcgggc atttggaagg cgccgtgagc attcccgcac
     3541 agcacttgct ggagtctaaa agcctcaaag tttttgacaa caaaaaagca acgattgtac
     3601 tctatggtcg ccggcaggaa caggcgcacg gtccctggct cctgctccgt caattgggct
     3661 acgatcatgt aaaacttctc caggccggtt acgacggcat cgcagccgct gattcgctga
     3721 gctttttagc gcccgaaacg gccctgttcg attacgcagc ttctttccgg caagctacca
     3781 ccgaaggcca aaaagccatt gaggcggtaa aaattgtgcc tactgccgtt gcccctgctg
     3841 ctcccaaaac catcatcacc aagcccaaag cagccgccaa accagcagcg gaagaggaag
     3901 agggatgttg aggagtatga gaaaaaatac tttttttcaa aaaaaacaac tcaatacttg
     3961 catcctattc aataatggca tacgtttgct gcgcataatt aaaaaagcgc aatgacagct
     4021 tcgtcaaatc agtggtactt tagcttttac ggcttttact tttacgctca agcgtaaggg
     4081 actgccgcca ttggttttca agaagttgaa actgtgaaat tgggagtccc gcaccggcga
     4141 ggctcccaat ttttgtttta tatcacaacc aaatcaaaat caaatgcaca ccacgcttgc
     4201 acctgcacaa gggttttacg gctggtttta tttttttggc acgggggact tgccctgaac
     4261 ggctttacgc attcgtttca gacagcaagt cccgcacaac acgggacttt ttttattctc
     4321 aaaacatagc accaacaaca tgatgatcgc aacagaaagt acagaaattg ccacacagga
     4381 ctcagaccaa cccctgcgtc gggtagccat tcagggcatt cccggcgcat ttcacgaggt
     4441 ggccgccagg ctatattttg atacagcgcc gctggaaata gtgccggccc tcacttttga
     4501 agacttagta cggcgattgg aacaaaacga cggcgtggac atcgccctga tggccataga
     4561 aaacacgctg gccggcagcc tgatgagtaa ttaccgcctg ctcgacaact cgcaactcag
     4621 cataaccggc gaattgcacc tgcgcattac tcaaaacctc atggcgctgc ccggccagtc
     4681 catccacgac attcaggaag tctattctca ccccatcgcc atagcgcagt gccgcgattt
     4741 ttttcgcgct tacccgcata tccgactcat cgaagcggaa gatacagccc tgagtgccaa
     4801 acagatcagc gaccgcaacc aatctgaggc tgctgctatc gccggcgctc tggccgcaga
     4861 aatgtacggg ctggaaatca tcgcgccggg catcgaaacc aacaagaaaa actatacgcg
     4921 cttcttagtg ctggagcgcc gggccgacgc gacagaaatc gttgatgccg acaaagtttc
     4981 tatttctttc agcgtggacc atgaggtggg gagcctgcac aaagtattgg cagtactggc
     5041 agcttacaac gtgaacctga gcaagataca gtcggcgccc atcatcggca agccctggga
     5101 gtatctcttt tttgtggatt ttgtaaccga aggcaaggtg ggctggaagc aggcgctgga
     5161 ggccattcag ccgctggcac acggcctcaa actgcacggg gcatatcgcc aaggaaagca
     5221 ttatgacgat tgaggcggtc atcagcaggc ggttgcgcca gggatagcag cgtaatggcg
     5281 gcggtcgggg atagagctgt tagctttagc attgcaatag ccggataaag ctaacagctc
     5341 tgcgggggcc gccatgtggc g
//
LOCUS       DAORLF010000340         5345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8403_length_5345_cov_2.091682, whole genome shotgun sequence.
ACCESSION   DAORLF010000340 DAORLF010000000
VERSION     DAORLF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8403_length_5345_cov_2.091682"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK198_20775"
     CDS             complement(<1..777)
                     /locus_tag="PK198_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF41245.1"
                     /translation="MSDVLVQKSQVLKGGEFLVKSSLPEDTFIPEEINEDQRMIYHMV
                     RDFLHQEIFPNYHRIEKQEPGFSASLLEKMGELGLLSAHIPEAYGGMGLDTNTNTFIG
                     DALGPAGSFTVSFAAHIGIGMLPIFYFGTEAQKLHYLPRLGSGELKASYCLTEPGSGS
                     DALAAKTRADLSPDGKHYILNGQKTFITNGPYADVIIVYAKLDEGDASVQGFSTVQVY
                     RRVIEGRAVQVVFSGDTVIERSYWGQGVVQRAYAALFLRVK"
     gene            1116..3830
                     /locus_tag="PK198_20780"
     CDS             1116..3830
                     /locus_tag="PK198_20780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF41246.1"
                     /translation="MSVPPIFISYRVKDTQIEARLLYVELANHFGRNAVFLDKKRIEP
                     GMDWPTELETKVAGCQVLLILVRKGVIWAGLNEDNGEYRINDPKDWVRREIETSNQSD
                     KEILPVFLDKAKFPAKSVLPEALHFLCTQQDKPIETENKWDDGVAALIKLLEGKGIRP
                     QKTGGENPVTLLDDYPLPAGINPFKKYKEPYIGMRYFDHDAAPLFFGRTRELNAFFSL
                     IERPETRLILVYGHSGVGKSSFLAAGVLPRLEHKVGDQNIHYERRDKTIQLGNQLQQL
                     LEKPREAAQKRIFILDQVEEMFTDPVKNERSRMVDILEAALQNNPADQFVLGFRSDYL
                     TDVQSLLAEIRTGSEAVHLEPLDRSAIVEAIEGVKRSEEKNRIFQYHLDFDDDFARKA
                     AADLIAKETAQVSTILQSRMQKLYLAAQERRAPSEPKVRLRWEDYEVLAKNAHAESEL
                     LDRAIEKVQQECSDAELHTIIETLDQFVINKETAGAKSKTDIENLESPLVQTLLHVNL
                     LTELRAKQAIRLAHDVLAPVVRRRREELDRSKYDSRTAQTFDLLWQNIQRGLETLDYE
                     AARQALSEAAQLQFYPKAYDLAPLAYELAYVFLWADKGTEGAALLRIFWDIQKQRGIL
                     FAPLPESSDPARLRDYLAACDPDLFMHLEHTYFPTMIPVQGGTFTMGSNEYKNEQPPH
                     EVTLSGFAIAQTPTTWQQFGIFCLETGFKVPHDEDWGRANRPLINVSWEDAAEFCRWI
                     STKRSRHYRLPTEAEWEYAARGGRLSKGCQYAGSDNLDEVGWYWENSGDERLSGEWGI
                     DKINKNKCRTHPVGQKRPNELGLYDMSGNVWEWCEDDWHDNYNGAPTDGRAWVGSSRG
                     DYRVYRGGSWAFDAVLCRAAYRNYWTPSNRVSYVGFRLVRSSPREIND"
     gene            complement(4353..4721)
                     /locus_tag="PK198_20785"
                     /pseudo
     CDS             complement(4353..4721)
                     /locus_tag="PK198_20785"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
     gene            complement(4718..5062)
                     /locus_tag="PK198_20790"
     CDS             complement(4718..5062)
                     /locus_tag="PK198_20790"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF41247.1"
                     /translation="MFFQNVFIALCRYFIFIEKEAIIATIRDYMSHQPVIWVGIAGSL
                     ARNEMTGNSDVDIVVQFDPAAQISLFDYVRYKQDLEEILERKVDILTYKYLSERLKPY
                     IERDIQIIYQAA"
BASE COUNT         1322 a         1411 c         1436 g         1176 t
ORIGIN      
        1 cttcacgcgg aggaagagcg cggcgtacgc gcgctgcacc acgccctggc cccagtacga
       61 gcgctcgatg acggtgtcgc ccgagaacac gacctgcacc gcgcgtccct cgatgacccg
      121 gcggtagacc tgcacggtcg agaagccctg cacgctcgcg tcgccctcgt cgagcttcgc
      181 gtagacgatg atgacgtcgg cgtacgggcc gttggtgatg aacgtcttct gtccgttgag
      241 gatgtagtgc ttgccgtcgg ggctgagatc ggcgcgggtt ttggcggcca atgcgtccga
      301 acccgaaccc ggctcggtga ggcaatacga ggctttcagc tcgccgctgc cgaggcgcgg
      361 gaggtagtgt agcttctgtg cttcggtgcc gaagtagaaa atgggcaaca tgccgatgcc
      421 gatgtgtgcc gcaaaagata cggtgaaaga accggccggc cccagcgcgt cgccaatgaa
      481 ggtattggtg ttggtgtcca agcccatgcc gccgtaagct tccgggatat gcgccgacaa
      541 cagtcccagt tcgcccattt tttcgagcag ggaggcgctg aagcccggtt cctgtttttc
      601 gatgcggtgg taattgggga aaatctcctg gtgcagaaaa tcgcgcacca tatgatagat
      661 catgcgctga tcttcgttga tttcttcagg aataaacgtg tcttcgggca gggaagactt
      721 gaccaagaac tcgccgcctt tgagcacctg cgacttttga accaatacgt cggacatggc
      781 tgatattatt tttggatatg agtaaatagc gaaaattcgc tacaaagata tacccgtttt
      841 tttgaattgt aaagaggaga gcgaaaattt tagcgaattt tcgctgaatt gagtggaagt
      901 gaggcgctca cttcgggtca gctcctcgct tggcgggcag gcaggcagtg cgaattcagg
      961 aggtaaccgg aagttaccac ctgacccccg ggcagcgcga ggcgctcact tcgggttagc
     1021 tcctcacttg gagagcaaca tttccccctt ttcccttatc ttgccccctg aaaatcaaat
     1081 tcgaagaaca ggtttggatt tgtaataaat agattatgtc cgtaccgcca attttcatca
     1141 gttacagggt aaaggatacc cagatcgaag cccggctttt gtatgtagaa ttagccaacc
     1201 actttggcag gaatgccgtg tttttggata aaaaacgcat cgaacccggc atggactggc
     1261 cgacagaact ggaaaccaag gtcgccggct gccaggtttt gctcatcctt gtgcgaaaag
     1321 gggtgatctg ggcggggctt aatgaggaca atggagagta ccggatcaat gacccgaaag
     1381 actgggtgcg gcgtgaaatt gagacatcaa accagtcgga caaggaaatt ctccccgttt
     1441 ttcttgataa ggcgaagttt cccgcaaaga gcgttttacc cgaagcgctg cactttttat
     1501 gcacccaaca agacaaaccc attgaaaccg aaaacaaatg ggacgacggc gtcgccgcgc
     1561 tcatcaaatt attggagggg aaaggcatcc ggcctcagaa aaccggcgga gaaaaccccg
     1621 tcaccctgct cgacgactac ccccttcccg ccggcatcaa tcctttcaaa aaatacaaag
     1681 aaccgtacat cggcatgcgg tacttcgacc atgacgcagc gccgctgttt tttggccgca
     1741 cccgcgaact caacgccttt ttcagcctca tcgaacgccc cgaaacacgg ctcatcttag
     1801 tgtatggaca cagcggcgtt ggcaaatcct cgttccttgc agcaggggtg ctgccccggc
     1861 tcgaacacaa agtaggcgac caaaacatac actacgaacg ccgcgacaaa accatccaac
     1921 tgggcaacca attgcagcaa ctccttgaaa aaccccgcga ggcagcccaa aaacgtattt
     1981 tcatcctcga tcaggtggag gaaatgttca ccgacccggt gaaaaacgaa cgcagccgta
     2041 tggtggacat actcgaggcg gcgctgcaaa acaaccccgc cgaccagttt gtgctcggct
     2101 ttcgaagcga ttacctgacc gatgtgcaaa gcctgctggc cgaaatccgc acgggcagcg
     2161 aggccgtcca cctggaaccc ctcgaccgca gcgccattgt ggaagccatt gaaggtgtga
     2221 aaagaagcga agaaaaaaat cgaatttttc agtaccacct cgattttgac gatgatttcg
     2281 cccggaaagc ggctgccgac ctcattgcca aagaaaccgc gcaggtcagc accatcctgc
     2341 agagccgtat gcagaagctc tacctcgctg cacaggagag gcgggcgccc agcgaaccca
     2401 aagtgcgcct gcgctgggag gactacgaag tgttggccaa aaacgcgcac gcagagtccg
     2461 aactcctcga ccgcgccatc gaaaaagtgc agcaggagtg ctccgatgcc gagctccata
     2521 ccatcatcga aaccctcgac caatttgtca tcaacaaaga aacagccggc gccaaatcca
     2581 agaccgacat cgaaaacctc gaaagcccgc ttgtccaaac cctgctccat gtgaacctgc
     2641 tcaccgagct gcgggccaaa caagccatcc gcctggcgca cgatgtgctt gcgccggtcg
     2701 tccggcgacg acgcgaggag ttggaccgca gcaaatacga cagccgcacc gctcaaacct
     2761 tcgacttgct gtggcaaaac atccaaagag gtttggaaac gctggattac gaggctgccc
     2821 ggcaagccct cagcgaggcc gcccaactcc agttttaccc caaagcctac gacctcgccc
     2881 ccctggctta tgagttggct tatgtatttc tatgggcaga caaaggcacc gaaggcgcag
     2941 cactactccg catcttttgg gacatccaaa aacaacgagg catcctcttt gcgcccctgc
     3001 cggaaagctc cgacccggcc cggcttcgcg actacctcgc cgcttgcgac cccgacctgt
     3061 tcatgcacct ggagcatact tacttcccca ccatgatacc ggtgcagggc ggtactttta
     3121 ccatgggcag caatgaatac aaaaatgagc aaccgccaca tgaggtcacc ctatccggtt
     3181 ttgcgatcgc ccaaaccccg acgacctggc agcaattcgg cattttttgc cttgaaacgg
     3241 gtttcaaagt gccgcatgat gaagattggg ggcgcgccaa tcgcccgctc atcaacgtca
     3301 gttgggaaga tgccgctgag ttttgccgat ggatttccac aaaaaggagc agacattacc
     3361 ggttgcctac cgaggctgaa tgggaatacg ctgcccgtgg gggcaggctc tcgaaaggct
     3421 gccagtacgc cggcagtgat aatttggatg aagtaggctg gtattgggaa aattcgggag
     3481 atgagcggct ttccggtgaa tggggtattg acaaaatcaa caaaaacaag tgccgaacgc
     3541 atccggtagg tcagaagcgg cccaacgaat taggcttgta cgacatgagc ggcaatgtat
     3601 gggagtggtg cgaggatgac tggcacgata attacaatgg tgcgccgacg gatgggcggg
     3661 cctgggtggg tagtagtagg ggcgactacc gcgtgtaccg cggcggcagc tgggctttcg
     3721 atgccgtgct ctgccgcgcc gcgtatcgca actactggac tccgtcgaat cgcgtcagct
     3781 acgtcggctt ccgcctcgtc cgcagttcac cccgtgaaat aaatgattaa tgccatgtat
     3841 ttttatgttt atgtcttacg cagtgaggtt gacggtaagt tatataccgg ttatactgct
     3901 gacttacgaa agaggttgga gagtcacaat aatggagagg tgaaatcaac aaaggcccgt
     3961 cgtccgttgg tacttgttta ttttgaagga tgtcttaatc agcaagatgc tactcacagg
     4021 gagcagtatt taaaaactac ctatgggaaa aggtatttga agaatcgtct aaagaattat
     4081 ttcacggggt aaaggaggtt tgctttcctg gcctaactat gagcaaaaga ggaagcgaaa
     4141 tgcacctacg gaatggagca aggagggggg agggacgatc cctccggcgg aatggagaag
     4201 gtgcatggag ctaagtaatg ttggcattga tggggcgtaa gccccgattt ttttttaaat
     4261 gctaataaaa gtaatcagcg tgccggtagc gtacagcgac agcatgaatg aggaactgaa
     4321 ccgctcgcgc cgggattcca cggcgtgagg cgctaacttc cggtcagctc ctcacttggg
     4381 ggtaaggatt tatccaaaat ggattgaatc tgcttttcca gcgccggcaa gtaaagcgca
     4441 atagtaaccc agatggtatt caggttcagc ccatcataat cgtggatgag aacatccctg
     4501 aagccggcca tttgttttca tggtatttcg gggctggcaa ggcgtatttc gtcagggatg
     4561 cgttttgcag cttctctcat aatttcaaat tttcgcacaa cggcgctgat aatgagatcg
     4621 ctatggtaga attcctcctc ggtgacgtcc cgggtaaagt ttaagatggc cgtgatagcc
     4681 tcatggatat gcgacaggta gatgacgggg tctttcttca tgcggcttgg tagatgattt
     4741 ggatatctcg ttcgatatag ggtttaagcc gctcgctcag gtatttatat gttaaaatgt
     4801 caacttttct ttccaggatt tcctccaggt cttgcttgta gcgcacatag tcgaataaac
     4861 tgatttgcgc agcagggtcg aactgcacaa cgatgtccac atcgctatta ccggtcattt
     4921 cattccgtgc taaagatcct gctattccca cccaaatcac cggctgatgt gacatgtaat
     4981 ctctgatggt cgctatgatg gcttcttttt ctatgaagat gaaatatcta cacaaagcta
     5041 taaagacatt ttgaaaaaac ataagatata gcggtttaat cagacttctt gttgcaaaac
     5101 acgcaccact agtgccgaga ttccatcttc aagtttacgc cgcagggcgg ggcgcgagta
     5161 ttacgcgggt tctacccgct tgtaaaacgc ccaatttcag ggcaatctcg cgctacgttt
     5221 tcctctacgg tcccctctcc atttgtcatt ccgtagcgta gcggaggaat ctgtaccatc
     5281 tccgcaggca gccagacgcg ggtgtcatgc gcggtggtac aggttcctac gccttgcctg
     5341 ccggc
//
LOCUS       DAORLF010000341         5334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8424_length_5334_cov_2.206289, whole genome shotgun sequence.
ACCESSION   DAORLF010000341 DAORLF010000000
VERSION     DAORLF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8424_length_5334_cov_2.206289"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK198_20795"
     CDS             complement(<1..479)
                     /locus_tag="PK198_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF41248.1"
                     /translation="MSKRSLPKWTNWILPVAVVLILAGRYIYFMPQLDSGENAPAFSA
                     TLPDGSRFQLDQLKGRYVLMHFWGSWCGPCRSENPQLVALYQRFGGEHFEIVSVGIEK
                     DPARWERAGKQDGLNWPFQLVEITPSFRFFDSPIANQFSVKKLPTLFLLNPKGEIVLV
                     "
     gene            complement(591..>2114)
                     /locus_tag="PK198_20800"
     CDS             complement(591..>2114)
                     /locus_tag="PK198_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41249.1"
                     /translation="SCNPTDPCNCPLVYDPVCVLGPDGLELHFPNACFAECAGFTAVD
                     FIDCEPDTTCVCPQIYAPVCVMSAAGDTITFINQCYAECEGYTADDIFSCNPSDSCNC
                     PLIYDPVCIALPGGPVMTFTNACFAACAGFSPDAFVDCDSLHFPDCYAAFYTEGADPV
                     GLAVQFTDASYAGDGTITSWNWHFGDPAGTTSTEQNPVHVYDAPGVYDITLTISTSDS
                     CTSTTTQHICIGGGGIVDSISCQAMFFFTQADSLGSAFQFQDMSFGNINSWLWNFGDG
                     TTSTEQNPAHTYTGNGAYVVTLTVSSDDCQSTVSMLVFAGDNIWYGNGFNALFVPFID
                     GLQVFFLNLTSGGSPSYTYEWNFGDGNTSSEFMPVYTYAEAGVYTVTLTVTDAAGVES
                     VFTATLNLITNEFTGRPQFLTASSTNETVERVSGLRAFPNPVSEQLTLTFEARSQEVY
                     RISILTLDGKEVQSAAAQMQAGRNQTTFDTRMLESGMYLLRLQTGSEVRTLKFVKVK"
     assembly_gap    2115..2124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2125..3008)
                     /locus_tag="PK198_20805"
     CDS             complement(<2125..3008)
                     /locus_tag="PK198_20805"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41250.1"
                     /translation="MKSILLSTIFACLSAMAWAQITVTVSGTVVFGQDQQPAPDYPVW
                     INSPVMDSTGANQNVVFTNANGVYTGIAVIGADMSLIVSTFDFCIGQVLNIELHIANP
                     AGGNLSNLNFHVCQDILPPPPPDGCAAFFIAEPADSASLTILFHDLSFSESPIQSWVW
                     DFGDGAAAEVPAPQHTYEEEGMYIVTLTITSDSCTATFTGVVYAGGDGEPCICPDVYD
                     PVCIATPAGGGFITFNNACEAECAGFTPDMFVECDTTGCLCPPFFDPVCVIDEAGDTL
                     TFTNYCYAECEGYTADDI"
     gene            complement(3021..3578)
                     /locus_tag="PK198_20810"
     CDS             complement(3021..3578)
                     /locus_tag="PK198_20810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41251.1"
                     /translation="MDDGAFVFQTQPYFTDLTSTTDVAITRFGQNPVTGIGPVAKAGI
                     IIEDDLIGLLITPADTIETFIRIKDVLVKDGEFGDLPIVTDSIRIVIRHPESLVRQRE
                     PQRPQMLHVFPNPTAGTVWISSPQPLLELALYNNAGQLVAQESLSGQYSRQWRLEHLP
                     SGMYVAHGRTEGGIFHQRIIISHHP"
     gene            complement(3509..>3976)
                     /locus_tag="PK198_20815"
     CDS             complement(3509..>3976)
                     /locus_tag="PK198_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41252.1"
                     /translation="GPVRLNATGGPTVQGIPLLWPAVFPDGVNYAYADADGNGVVDWH
                     DLLATFLNYGVQLDSVEEVVLQQGIPGVDARLGFQQSSSAFTIFPGDMLELPILLEFS
                     PNMPAAVNGLAFRLEFDDRYFEQIAISTEDSWMTALLFFRRSPILPTSPVQRM"
     assembly_gap    3977..4001
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4454..>5334)
                     /locus_tag="PK198_20820"
     CDS             complement(4454..>5334)
                     /locus_tag="PK198_20820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41253.1"
                     /translation="GVTPLTVTPTASGTLYLHINTNAACGTQSVSRTTTVACSSCIAP
                     PAPANDDCSGAIALTVGTTCSFTQYTTAAATASAGVPAPGCANYAGGDVWFSVVVPAS
                     GHLILDSQTGVITDGGMAIYSGSCGSLTLIECDDDDSANGLMALINRTGLTPGSTIYV
                     RFWEYGNDNPGTFSICARNGTPPTLTAPTNTTAELAGEVVGLPEGALTVGQVFPNPMV
                     GSTANIRVDSPKETEAIVRFMDQMGREVMMIESDLNVGDNLLQLNISRLPAGTYFVMV
                     QVEGKVIPRRLVVPRS"
BASE COUNT         1314 a         1370 c         1457 g         1158 t
ORIGIN      
        1 acgagtacta tctcgccctt cggattcagt aaaaacaaag taggcaactt tttgacgctg
       61 aactgattgg ctatcggcga gtcgaaaaaa cgaaaactcg gcgtaatttc taccaattgg
      121 aagggccagt tgaggccatc ctgcttcccg gcgcgctccc accgggcagg gtctttttca
      181 atgcccacgc ttactatttc aaagtgttcg ccgccaaaac gctgatacag tgctacgagt
      241 tgtggattct ctgaacgaca ggggccgcac cagctccccc agaagtgcat caacacatac
      301 ctccctttca gttgatcgag ttggaaccgg gaaccgtcgg gaagcgtggc gctgaatgca
      361 ggagcgtttt cacccgaatc caattgaggc atgaaataaa tatagcgacc ggccaatatg
      421 agcacgacgg cgacggggag tatccagttg gtccacttgg gcaaagatcg tttcgacatt
      481 gcttgatgat gttgattcaa cgcagaaaca ccgccgccca caaataggtt ggacacaaaa
      541 aaagagggcc tctctctcga aaggccctcc ttgtttttat acatgatgca ctatttcact
      601 ttgacgaatt tcagagtgcg cacttcgcta cctgtttgca ggcgcagcag gtacattccg
      661 ctctccagca ttcgggtgtc gaaggtggtt tggttgcgcc cggcctgcat ttgcgcggca
      721 gcgctctgta cctccttgcc gtcgagcgtc agtatggaga tgcgataaac ctcctggctg
      781 cgagcctcga aagttaaggt cagttgttcg gaaaccgggt tggggaatgc ccgaagcccc
      841 gatacgcgtt ctacggtttc attcgtcgaa ctggctgtca ggaattgcgg gcgcccggta
      901 aactcattgg taatcagatt cagcgtggca gtgaatacgc tttcaacgcc cgccgcatcc
      961 gtcaccgtca acgttacggt gtaaacgccc gcttcggcgt aggtgtacac gggcatgaac
     1021 tcgctgctgg tattgccatc gccgaaattc cactcgtaag tgtaagaagg tgatccgccg
     1081 gaagtaagat tgaggaagaa cacctgtagc ccgtcaataa aaggcacaaa caaggcattg
     1141 aaaccattgc cataccagat attgtcacct gcaaaaacca acatggaaac tgtactttga
     1201 cagtcgtcgc tgctcactgt cagcgtcact acatacgcgc cgttgccggt ataggtatgc
     1261 gccggatttt gctcggtgct ggtagtcccg tctccgaagt tccacagcca ggagttgata
     1321 ttcccaaaag acatatcttg aaactggaag gcgctgccga gcgagtctgc ctgcgtaaag
     1381 aagaacatgg cctggcagga aatgctgtcc acaatgccac cgccgccaat gcagatgtgc
     1441 tgcgtagtgg tggaggtaca ggaatcgctg gtgctgatgg tgagggtgat gtcgtaaacg
     1501 ccgggagcat catacacatg cacgggattt tgctccgtac ttgttgtacc tgctgggtcg
     1561 ccgaaatgcc agttccagga ggtaatcgta ccgtctccag cgtaagaagc atctgtgaat
     1621 tgcacggcca gaccaacagg gtcggcccct tctgtataaa acgcggcata acagtcgggg
     1681 aagtgcaggc tgtcgcagtc cacaaaagca tcgggactga atccggcaca cgctgcaaag
     1741 caggcgttgg tgaaagtcat gacagggccg cccggcaatg cgatacagac cgggtcgtaa
     1801 atcaaggggc aattgcagga atcgcttggg ttgcaactga aaatatcgtc ggcggtatag
     1861 ccttcacatt cggcataaca ctgattgatg aaagtgatcg tatctcctgc ggcagacata
     1921 acgcaaaccg gggcataaat ttgtgggcaa acacaggtgg tatccggttc acaatcaata
     1981 aaatcgacgg ctgtgaaccc ggcgcactca gcaaagcacg cgttcgggaa gtgcagttcc
     2041 agcccgtccg ggccaagtac gcataccgga tcataaacca atgggcaatt gcagggatcg
     2101 gttggattgc agctnnnnnn nnnnaaaata tcgtcggcag tgtagccctc gcattcggca
     2161 tagcagtaat tggtgaacgt cagcgtatcg cctgcctcgt caatgacgca aaccggatcg
     2221 aagaacggcg ggcaaagaca gccggtcgta tcgcattcca caaacatatc cggcgtgaat
     2281 ccggcgcact cggcctcgca ggcattgttg aaggtgatga agccgcctcc agccggagtg
     2341 gcaatgcaca ccgggtcata tacatccggg cagatacagg gttcgccgtc accgcctgca
     2401 tacaccactc cggtgaaagt ggcggtgcat gaatcggagg tgatggtgag ggtcacaatg
     2461 tacatacctt cttcttcata ggtgtgctgt ggtgccggga cttcagcggc agcgccgtct
     2521 ccaaaatccc atacccaact ctgaatgggg ctttcgctga atgacaagtc gtgaaacagg
     2581 atcgtcagcg aagcactgtc agccggttcg gctatgaaaa aggcagcgca gccgtcgggc
     2641 ggcggcgggg gcaggatgtc ctgacataca tggaaattca ggttgctgag atttccgccg
     2701 gcagggttgg cgatgtgcag ttcaatattc agcacctgtc caatgcaaaa gtcaaaagtg
     2761 gaaacgatga ggctcatgtc tgcgccaata acagcaatgc cggtataaac gccattggca
     2821 ttggtaaaca ccacattttg attagcgccg gtgctgtcca ttaccggaga attgatccac
     2881 acaggataat cgggcgcagg ttgttggtct tgcccgaaaa caaccgttcc gctgaccgtc
     2941 acggttattt gcgcccaggc catagcggac aggcacgcaa aaatggttga caataagatg
     3001 ctcttcataa taaagcgatt ttatgggtga tgagaaatga tgatgcgttg atgaaaaatg
     3061 ccgccttcgg tgcggccatg cgccacatac atcccggaag gcaaatgctc cagacgccac
     3121 tgacggctgt attgcccgct cagggattcc tgcgcaacca actgcccggc gttgttgtac
     3181 aacgcgagtt ccagcaaagg ctgaggcgat gatatccata ccgtaccggc ggtgggattg
     3241 ggaaaaacat gcaacatttg agggcgctgc ggctcgcgtt gccgaaccag actttcaggg
     3301 tgtcgaataa cgatgcgaat gctgtcggtg acaatgggga ggtcgccaaa ctcgccgtct
     3361 ttgaccaata cgtccttgat gcgaatgaag gtttcaatgg tgtctgccgg cgtgatgagc
     3421 aacccgatca ggtcgtcttc gatgatgatg ccggccttgg ccaccggccc gatacctgtg
     3481 acggggtttt gcccaaaacg cgtaatggct acatccgttg tactggtgag gtcggtaaaa
     3541 tagggctgcg tctgaaaaac aaaagcgccg tcatccaact atcttccgta ctaatggcta
     3601 tctgttcaaa gtaccggtcg tcaaactcca acctaaaggc cagaccgttg acggcggcag
     3661 gcatattggg cgaaaattcc aataaaatgg gcaactccag catatcgccg gggaaaatgg
     3721 taaaagccga actgctctgc tgaaatccca gccgagcgtc cactccgggg ataccctgct
     3781 gcaaaacaac ttcctccacg ctgtccaatt gaacgccata gttgaggaat gtggccagca
     3841 agtcgtgcca gtccaccacg ccgttgccgt cggcgtcggc ataggcatag ttgacgccat
     3901 cgggaaaaac ggcaggccag agcaggggaa tgccttgtac agtagggccg ccggtagcat
     3961 tgagcctcac agggccnnnn nnnnnnnnnn nnnnnnnnnn naagcatagc cggtgtgcag
     4021 caagtctaaa tagtccaccc ggccgttgtt gttggcgtcg ccgggcagga tggcgttttg
     4081 cccccgaatg caggcccaac tcaggcacaa cagagccgtt gccgcaataa ttcttattcg
     4141 atagtagctg ttcataaatg gtacctgtcc ttttgcgaat gtaaaggtac gggctgcgca
     4201 gaggggattc aatgtcgttt ttttgatgcc tttcctgata atatcaatca ataattgatt
     4261 caattacctt ttaatcaatt atatagcata taaataaatc atttttttat caccaattct
     4321 gagatcttaa caaaaagtta ttgtcatttg accgacgtct ccgcccggcc ccaaaaaaca
     4381 aacagcgccc gacgagtgcc ggacgctgcc tgtgtttttt caaatcaaag gttcttgcga
     4441 acctggtgcg gtattatgat ctcggcacca ccaagcggcg cggaatcact ttgccttcta
     4501 cctgtaccat cacgaagtag gtacctgccg gcagacggct gatgttcaac tgaagcaggt
     4561 tgtcgcctac gttcaggtca gattcgatca tcattacttc gcggcccatt tggtccatga
     4621 agcgaacgat ggcttcggtt tccttcggag agtccacgcg gatattggct gtgctgccca
     4681 ccatcgggtt ggggaacacc tggcctaccg tgagagcgcc ttcgggcaaa cctaccactt
     4741 caccggcaag ttcagcggtg gtgttggtgg gcgccgtaag cgtcggagga gtaccgttgc
     4801 gcgcgcaaat gctgaaagta cccgggttat cattgccata ctcccagaaa cgcacataaa
     4861 tggtagaacc aggagtaaga ccagtacggt tgataagagc catcaagccg ttggcgctat
     4921 catcgtcatc gcattcaatg agcgtcaaag aaccgcaaga gccggaatag atagccatgc
     4981 cgccgtcggt gataacgcct gtctgcgagt caaggataag gtggccgctg gccggaacta
     5041 ctacactgaa ccaaacgtcg ccgcccgcat aattagcaca acccggtgcc ggaacgcctg
     5101 cagaggctgt ggccgcagca gttgtgtatt gcgtaaagga gcaagttgta cctaccgtca
     5161 gagcgatagc gccggagcag tcatcgttgg cgggagctgg aggtgcaatg caggaagaac
     5221 atgctaccgt cgttgtacga gacacagatt gagtgccgca tgcagcgttc gtattgatgt
     5281 gcagatagag cgttccgctg gcagtaggag ttacggtgag cggcgttacg ccac
//
LOCUS       DAORLF010000342         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8527_length_5280_cov_1.690335, whole genome shotgun sequence.
ACCESSION   DAORLF010000342 DAORLF010000000
VERSION     DAORLF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_5280_cov_1.690335"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>224)
                     /locus_tag="PK198_20825"
     CDS             complement(<1..>224)
                     /locus_tag="PK198_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRF41254.1"
                     /translation="PNHIFALLTDGGTYNSQTISGLGLTKTMHLFGLARQYLTRTGDF
                     GNMADALETACAALVGINLEGISLTATPAG"
     assembly_gap    225..330
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<331..>667)
                     /locus_tag="PK198_20830"
     CDS             complement(<331..>667)
                     /locus_tag="PK198_20830"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRF41255.1"
                     /translation="AEMRGIYNASISCPNANWNGTTTNFCTNVASDDVVAHEWGHAYT
                     EFGPGLIYAWQAGALNESYSDIWGEVVDLLNAYEDSGEDLSLRTGCGTSLRWMMGEDA
                     SAFGGAIRDM"
     assembly_gap    668..677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<678..>1027)
                     /locus_tag="PK198_20835"
     CDS             complement(<678..>1027)
                     /locus_tag="PK198_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41256.1"
                     /translation="FPDRPLLAHTHGVFWYHAGLAQGIPGEPILAYEVEVANDADLRQ
                     LVYVDVVKGAVADRLPGTCELLFRRVYNVNTGTQVWQEGNAFPGSLSLMQQNMVEAAG
                     HTYYLFKNLFGLDS"
     assembly_gap    1028..1066
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1067..>1443)
                     /locus_tag="PK198_20840"
     CDS             complement(<1067..>1443)
                     /locus_tag="PK198_20840"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41257.1"
                     /translation="ETQGTYINYHRSTGAPAFVRWPDHAPLALPGNNLMEKAAAFFET
                     YGRVFGIQEPARELAFVVENKDKTGYTHLKFKQLHLDTEVFGGEVLLHFDADGMLHTV
                     NGVFIPGIRQQYAQPLQTSAQAEA"
     assembly_gap    1444..1508
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1509..1705
                     /locus_tag="PK198_20845"
     CDS             <1509..1705
                     /locus_tag="PK198_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41258.1"
                     /translation="QEAPSDGQIQAAILKKRGKVSKHEQLIVLIGAIMDSFQFKTMNS
                     TPSLTRYLATGKNKRIALHL"
     gene            complement(1795..>2659)
                     /locus_tag="PK198_20850"
     CDS             complement(1795..>2659)
                     /locus_tag="PK198_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41259.1"
                     /translation="LGLLPEVLANSGAMQKVYLKTDKGAILSRSEPRYEYPTVCIHRA
                     DLHRILLQNVPAQLYTHHALSDISHLENGQVALRFENGETRNFDAVIGADGIHSVVRQ
                     HIINDGKPIFRGYNVWRGVVATRFDIGYGSETHGNGKRVGIVPVKDGVYGWWATCNED
                     FMQSDEPEGSKNKLLRLFGDWHDPIPELMANTEHIIKTPWPTGSLCAAGRRGRLRCWA
                     MRRTPPRPIWGKAAAWRWRAPILWPKPLSATASARRHFSDTRICIFPALRELCWKAGG
                     WGLSGNSLNLC"
     assembly_gap    2660..2738
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2896..>3655)
                     /locus_tag="PK198_20855"
     CDS             complement(2896..>3655)
                     /locus_tag="PK198_20855"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF41260.1"
                     /translation="THKAEDATARKISLDGLGGKYSDLWLYTYLLEPTLVNPSSKKVS
                     YSHLFPKPLNPSIFQQLVAQQLPDMPAAEADALWPDLLRQADALSENLKQDNVLVEDR
                     TEILALLAYLQQIPPSPYQMKLDSIARAEELKKSAALFDALQNDKSIVFTTPATKTNI
                     EMGRSIFQPNCSACHGMRGEGGIGPNLTDKSWRHGSTKRDIANTIVHGVPERGMISWK
                     NMLSPEEVGLLVVFIRSIKGSNPPAAKKKQGKKG"
     assembly_gap    3656..3669
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3977..4088
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4089..4713
                     /locus_tag="PK198_20860"
     CDS             <4089..4713
                     /locus_tag="PK198_20860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRF41261.1"
                     /translation="VGISTYATHEAWEGLENASTQTPSVLKLEMSEHSGTHVDALSHM
                     GRPYAAQSIDNMPLTMFYTGGICLDFRHKGLSEMIHAHEIEAAIADTGLAVQKGDTIL
                     LCTDHYRKHFATENWPNGPGISAETARLLGGMGIAAFGVETMSPGIPKVSNKAVHQIC
                     GELGFTHYENLINLNRLLGRGRFRFIALPLKIRGGTGSPVRAVAVLE"
BASE COUNT         1115 a         1238 c         1327 g         1175 t
ORIGIN      
        1 ggccggccgg cgttgcggtg agcgagatac cctccagatt gatgcccacg agtgccgcgc
       61 aagccgtttc gagcgcatcg gccatattcc cgaaatcgcc ggtacgggtg aggtattgcc
      121 gggctaagcc gaacagatgc atggttttgg taagccccag cccggagatg gtttggctgt
      181 tgtaagtgcc gccgtcggtg agcaacgcga agatgtgatt gggtnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catatcccgg atggcgccac cgaacgcaga
      361 ggcatcttcg cccatcatcc agcgcaggga agtgccgcag ccagtgcgca gcgagaggtc
      421 ttcgccgctg tcttcgtagg cgttgaggag gtccaccact tcgccccaga tgtcggagta
      481 cgattcattg agggcgccgg cctgccaggc gtatatgagg ccggggccga attcggtgta
      541 ggcatggccc cactcgtgcg ccactacgtc atcagaggct acattggtac aaaagttggt
      601 agtagtgccg ttccaattcg cattaggaca ggaaatagaa gcattgtaaa ttccacgcat
      661 ctccgctnnn nnnnnnngga atccaagcca aagaggtttt taaaaagata gtaggtatgc
      721 cccgccgcct cgaccatgtt ttgttgcatg agtgacagcg agcctggaaa agcattgccc
      781 tcctgccata cctgcgtacc ggtattgaca ttatataccc tcctgaagag cagttcgcaa
      841 gtgccgggca ggcggtcggc tactgcgccc ttgacgacgt ccacatatac caattgccgg
      901 aggtcggcgt cgttggccac ttccacttca tacgccaaaa tgggttcgcc gggaatgccc
      961 tgcgccagtc ctgcatggta ccagaacacg ccatgcgtat gggccagcaa agggcggtcg
     1021 gggaaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcct ccgcctgtgc
     1081 gctggtttgc aggggctgcg cgtattgctg ccggatgccg ggaataaaaa cgccgttgac
     1141 ggtgtgaagc attccgtcgg catcaaaatg gagcaagacc tcaccgccga agacttccgt
     1201 gtcgagatgc aattgtttga atttgagatg tgtataaccg gttttgtctt tattttcaac
     1261 aacaaaagcc agttcgcggg caggttcctg tattccaaaa acgcggccat aagtttcaaa
     1321 aaatgcggct gctttttcca tcaggttatt gccgggcaaa gccaatggcg cgtgatcggg
     1381 ccagcggaca aacgcgggcg ctccggtgct ccggtgataa ttgatgtacg ttccctgagt
     1441 ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnag caagaggccc caagcgatgg tcagattcag gcggctattc tgaaaaaaag
     1561 aggaaaagtg agcaagcatg agcagcttat tgtattaatt ggtgcaatta tggactcttt
     1621 ccaattcaaa acaatgaatt caacgccatc acttacacgg tacttggcga caggcaaaaa
     1681 taagagaatt gccctacacc tctaatttcg tcacccggta ctcaaaatat ttgctcatca
     1741 atttcatggc cagactggac ggcgtacatc ggaatgccag gtttcggagt gcaatcaaca
     1801 gaggttgaga gagttgcccg acaaccccca gccgcctgct ttccaacaca attctcttag
     1861 cgcggggaaa atgcaaatcc tcgtatcgct gaaatgcctg cgagccgagg ccgtagcgct
     1921 caatggcttt ggccaaagta taggcgccct ccatcgccat gcagccgcct tgccccagat
     1981 tgggcgtggt ggggtgcgcc gcatcgccca gcagcgtagc ctgccccttc gaccagccgc
     2041 gcacaggctc ccggtcggcc agggcgtttt gatgatgtgc tctgtattcg ccatcagttc
     2101 gggaatggga tcgtgccagt cgccaaacaa gcgcaggagt ttgttttttg atccttcagg
     2161 ctcgtcgctt tgcataaaat cttcgttgca ggtggcccac cagccataca cgccgtcttt
     2221 gaccggcacg atgcccacgc gtttgccgtt gccatgcgtt tcgctgccat agccaatgtc
     2281 aaaacgggtg gctaccacgc ctcgccaaac attgtagcct ctgaaaatcg gctttccatc
     2341 attgataata tgctgtctca caacggagtg tatcccatca gcgccgatca cggcatcaaa
     2401 gttgcgggtt tctccatttt caaaacgcag cgctacctgc ccgttttcca gatgagaaat
     2461 atcgctcaac gcatggtgtg tgtaaagctg agcgggtacg ttttgcaata aaatgcggtg
     2521 cagatcggcc cggtgaatac aaacggtcgg gtattcatac cggggttcgg accggctcaa
     2581 aatggcgcct ttgtcggttt ttaaatacac tttttgcatg gcgccgctgt tggccagtac
     2641 ttccggcagc aatccgaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct cctttttttc aaaaatatgg
     2761 agctcgtaca tttcgttgcc caagctgcgg gcaaaagtga ggccggcaat tccgccgccg
     2821 atgatcgcta tttgtttttt gtggcgcatg tgctttggat gtgaatggat cagatataat
     2881 gagcggagga gcaactcacc cttttttacc ctgttttttc ttggcggcag gcggattgct
     2941 acccttgatg gaacggatga atacaactaa caggccgact tcttccggac tcaacatatt
     3001 tttccatgag atcatccccc tttccggtac tccgtgtacg atggtattgg caatatcccg
     3061 ttttgtgctg ccgtgccgcc aggatttatc tgtcaggttg gggccgatgc ctccctcgcc
     3121 tctcattccg tggcaggcag aacagttagg ctggaaaatt gatcttccca tttcaatatt
     3181 cgtcttggta gccggcgtag tgaatacgat gctcttgtcg ttttgtagcg cgtcaaatag
     3241 cgcggcgctt ttttttagtt cttctgcgcg ggcaatgctg tccaatttca tttggtaagg
     3301 gcttggagga atttgttgca aatacgccag aagcgccaag atttccgttc tgtcttcgac
     3361 gagtacattg tcctgtttca ggttttcgga aagggcatcg gcttgtcgga gcaagtcagg
     3421 ccataaagca tcggcttcag cggccggcat gtcgggcagt tgttgggcaa ccaattgttg
     3481 gaagatggat ggattgagcg gcttggggaa caggtgggaa taggaaacct tttttgagga
     3541 tgggtttacc aatgtgggtt ccagcaggta ggtataaagc cacaagtcgg aatattttcc
     3601 gcccagtccg tctaagctga tttttcttgc tgtagcatcc tcggctttgt gcgtgnnnnn
     3661 nnnnnnnnnc gtgtggcatc tttggcaatt ttctctttgg taaatgtact cgccgtaaat
     3721 ggccaaaccg gatgtaggat cgggagcctt gccgggttcg agaggctcgg attgaataga
     3781 aaagctgttg gaaagcaata caaccgccgg caaaatcagt acaaagagcc aggtattttt
     3841 catgctgttc tgagtattga gttataaata ccctcaaaga aaagcatttt tccagatata
     3901 aaaaggagta acgcatcaaa tcgggaatgg cctcggatgg caggcataga taaaatggtg
     3961 ttggcgatgt tttgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnncg tcggcatcag cacctacgcc acccacgagg catgggaggg cttggaaaat
     4141 gccagtaccc aaacgcccag cgtattgaag ttggaaatga gcgaacactc cggcacacat
     4201 gtagatgccc tcagccacat gggccgccct tatgcggcgc aatctattga caacatgccg
     4261 cttacgatgt tttacaccgg cggcatctgc cttgattttc gccacaaagg attatcggaa
     4321 atgatccatg cacacgaaat cgaagcggcc atagccgaca ccggattggc cgtgcagaag
     4381 ggcgatacca tcctgctgtg taccgatcat tatcgcaagc actttgctac ggaaaattgg
     4441 ccgaacgggc cgggcatcag cgcagaaacg gcgcgtttgc tgggcggcat gggcattgcg
     4501 gcgttcgggg tagaaaccat gtcgccgggc atcccgaaag tctccaacaa agccgtccac
     4561 cagatttgtg gggaactggg ctttacgcat tacgaaaacc tgatcaatct caatcgccta
     4621 ctggggcgcg gccggtttcg gttcatcgcc ttgccgctca aaatccgggg cggaaccggc
     4681 tctcctgtaa gggcagtggc ggtgttggag tgagggagga taagccccac caaacccaga
     4741 tatgagtatc taaaaccatc atgcgagtac gttccagcca tcttcggcga ccggttccaa
     4801 agggaggtca taatgtaata ccgagccttg taaaggcgct gccgactgta aattgggcgg
     4861 cgcggactgc atggcggcgg ctagggcgtt aaaaatgatt gccttgtttt ccggagacaa
     4921 ttgagtgaaa aactacagga actgccccag atcgaatttc agcggtatgg ttaagttgtt
     4981 cattacatat tttttagcac tacaaagtaa ggtttttttt atcaattctc aaaatctgcg
     5041 aatgaatcgt gggtttttgt tgccccgata agtcagccgg tatcccaccg ctacagtctt
     5101 ggttatccgc ctgaaaagtc cactggactt ttctcggatt gtcggcctgc ggagcacgcc
     5161 tccagcccac gtcgaagggc atgatgcttc gggaaattgc acctgtttac gacggcatac
     5221 gagcgaggtg acatctgccg tcgccgcccg gacgactcca atggagggaa cctcctggcg
//
LOCUS       DAORLF010000343         5267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8560_length_5267_cov_1.860514, whole genome shotgun sequence.
ACCESSION   DAORLF010000343 DAORLF010000000
VERSION     DAORLF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8560_length_5267_cov_1.860514"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PK198_20865"
     CDS             <1..1553
                     /locus_tag="PK198_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRF41262.1"
                     /translation="QDQIARIIEGEETAFLHTLENGIRRFESLETPGNTVRGKDAFDL
                     LDTYGFPIDLTRLMAQEKGLTVDEEGFAKALEEQKQRARADAQKEVGDWVSLSDDASV
                     TFVGYDALSVEQARIVKHRTVKVKGKDQYQLVLNVTPFYAESGGQAGDTGVLTVGAEK
                     IQVIDTQKENDLIIHVVNRLPADPKAVFHAQVDAEKRRAVSSNHSATHLMHAALHEVL
                     GNHALQKGQDVTPDRLRFDFSHFQKVSDEEIKAIEAMVNAKIRENIALEEDRAVPIEE
                     AKKLGAMMLFGEKYGEFVRVITFDKNFSRELCGGTHVAATGQIGQFKIVAESAVAAGI
                     RRIEAVTAGGAEAYFAKEMAELNAVRETLKNPQNLLAAVQSLQEENRRLGKEIERLIA
                     EQAGALKGELMARVEQSGDIRFISAVLPLNDANAIKTLAYQLEKELGNAVVVLGAVVN
                     EKPLLTVIISQNLVDTKGLHAGNMVRELAKEIQGGGGGQPFFATAGGKDANGLEKAVQ
                     KAKTTLPA"
     assembly_gap    1558..1764
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1765..2077
                     /locus_tag="PK198_20870"
     CDS             <1765..2077
                     /locus_tag="PK198_20870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF41263.1"
                     /translation="TIQFALDIIAYCEILEQQRKFVVSRQLLKAGTSVGANVHEAQNA
                     ESRADFIHKMKVAAKEANESEYWLIICQLSPSYPDCEKLLADARTILKVLSKIIGSSK
                     K"
     gene            2131..3045
                     /locus_tag="PK198_20875"
     CDS             2131..3045
                     /locus_tag="PK198_20875"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRF41264.1"
                     /translation="MSHQHIIEGIYSDVQPFFGRGKVADYIPALARVRPDQLGIALQT
                     LDGQTAVAGDSEIRFSIQSISKTFTLALAFALEGNDLWKRVGVEPSGNPFNSLVQLEF
                     ENGKPRNPFINAGALVVTDVLCRHYDDPKSVILNLIRSLAGSDDISFDEEVAASEKAT
                     GFRNQSLAWFLKSCGNLHSDVDTVLDVYFHQCSIAMNCRELSRAFLFLANHGVHPYTG
                     EAMLTRSQAKRLNAILLTCGFYDQAGEFAFRVGMPGKSGVGGGVAAFIPGELAVAVWS
                     PELNEHGNSVVGMQVLEWFTTRTGRSIF"
     gene            3278..4357
                     /locus_tag="PK198_20880"
     CDS             3278..4357
                     /locus_tag="PK198_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRF41265.1"
                     /translation="MKIRSVSTWKENLDLSKPYTIAYKTIDHVENLFVVVELENGIIG
                     VGSGSPAEFVTGENIHASAEALSGLVEGLLLKQDIRHYKRLIRDAAAQLPARPAALAA
                     IDIALHDAFAQLLGLPLADYLGRVYQGFPTSVTIGIQSVEQTLEDARAFVADGFRVIK
                     LKTGVSAEQDIETFSRLRETVGPDITIRVDVNQGYKTSDLLIFDYAVRDLNVEFYEQP
                     LPPNNLDEMLLLPPDLCAFCAADEDLLTMEDAVQMAHHPQPYGIYNIKLMKCGGVDHG
                     LKIAEVAEMAGIHLMWGCNDESVVSISAALHAALASTNTRYIDLDGSFDLARDIARGG
                     FVLRDGCLYDNGKPGLGAELLAASL"
     gene            4471..4773
                     /locus_tag="PK198_20885"
     CDS             4471..4773
                     /locus_tag="PK198_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41266.1"
                     /translation="MDILAEIREERRRQDAKWGEQNHSPIEWSAILTEEVGEVSKEAL
                     ELHFAQLQNTDKSEDWYAEKLAAYRKEVIQVAAVALAMAESLERNKWRTLRRGESQ"
     gene            complement(4767..>4966)
                     /locus_tag="PK198_20890"
     CDS             complement(4767..>4966)
                     /locus_tag="PK198_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41267.1"
                     /translation="RKIFDTTPCVVNMKVEPRAGIYFMFTDFRPAATPSTYFFPLARD
                     SISCDLSCGFYPPSKLFFHLH"
     assembly_gap    4967..5052
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5053..>5267)
                     /locus_tag="PK198_20895"
     CDS             complement(<5053..>5267)
                     /locus_tag="PK198_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRF41268.1"
                     /translation="VPAAKSSDNFRWSAAVAEFGMLLRNSEFKGKATFEQCIQLAKKA
                     RGDDEKGYRQECVRLMETAAALGGVGE"
BASE COUNT         1243 a         1299 c         1381 g         1051 t
ORIGIN      
        1 agcaggacca aatcgcccgc atcatcgaag gcgaagaaac cgccttcctg cacaccctcg
       61 aaaacggtat tcgccgattc gagagtttgg aaacgcccgg caatacagtg cgcggaaaag
      121 acgctttcga cctgctcgat acctacggct tccccattga tctcacccgc ctcatggccc
      181 aggaaaaagg gctgacggtg gacgaggagg gctttgccaa agcgctggag gagcaaaaac
      241 aacgcgcccg cgccgatgcc caaaaggaag tgggcgactg ggtttccctg agcgacgacg
      301 catcggtcac tttcgtggga tacgatgcgc tcagcgtgga gcaggcgcgc attgtgaagc
      361 accgcaccgt gaaagtgaag ggcaaagacc agtaccagtt ggtgctcaac gtgacgccgt
      421 tttacgccga aagcggaggt caggcaggcg acacaggcgt actcactgtc ggtgcagaga
      481 agatacaagt cattgataca caaaaggaaa acgacctgat tatccatgtc gtcaaccgct
      541 tgccggccga tccaaaggct gtgttccatg cacaggtgga cgccgaaaaa cgccgggctg
      601 tttccagcaa tcacagcgcc acgcacctca tgcacgccgc tttgcacgag gtattgggca
      661 accacgccct gcaaaaaggc caggatgtga cgcccgaccg cctgcgtttc gacttctcgc
      721 atttccagaa ggtaagcgat gaggaaatca aggccattga ggcgatggtg aatgccaaaa
      781 tccgcgaaaa catagcgctg gaagaagacc gcgccgtgcc catcgaggag gccaaaaaat
      841 tgggcgccat gatgttgttc ggcgagaaat acggcgaatt tgtgcgcgtc atcacctttg
      901 ataaaaactt ctcgcgcgag ttgtgcggcg gcacacatgt ggcggctacc gggcagatcg
      961 ggcagttcaa aatagtggcc gaaagcgctg tagcagcggg cattcgacgc atcgaggccg
     1021 tgactgccgg cggggccgag gcttattttg ccaaagaaat ggccgaactg aacgccgtgc
     1081 gtgaaacgct caaaaatccg caaaacctgc tggcggccgt gcagtcgctg caagaggaaa
     1141 accgccgttt gggcaaagaa atcgaacggc tcatcgctga gcaggccgga gcgctgaaag
     1201 gcgaactcat ggcccgggtc gagcagtcgg gagacatccg tttcatcagc gcggtgctgc
     1261 cgctcaacga cgccaatgcc atcaaaacac tggcttatca gttggaaaaa gagttgggca
     1321 atgcggtggt cgtattgggc gccgtggtga acgaaaagcc attgctgacg gtcatcatca
     1381 gccaaaactt ggtggatacc aagggtttac atgccggaaa tatggtacgc gaattggcta
     1441 aggaaattca gggcggcggc ggcggacaac cgttttttgc aacggccggc ggtaaggatg
     1501 cgaacggatt ggagaaggca gttcaaaaag ccaaaaccac gctgccggcg tgatctannn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnngaccat tcaatttgca ttggacatca ttgcttattg
     1801 cgaaatattg gagcagcaac gtaaatttgt agtgagtcgc caattgctga aagccggaac
     1861 ctctgtcggc gcgaatgttc atgaagctca aaatgccgag agcagagctg attttattca
     1921 taaaatgaag gtggcggcta aggaagcgaa tgaatcggaa tattggctca ttatttgcca
     1981 attatcgccc agctatccag attgtgagaa gttattggca gatgcaagga ctatattgaa
     2041 ggtactttcg aaaataatag gttcttcaaa aaaatagatc atcataagat tcctcgaact
     2101 tatcgcgaga ggaatcagca cattatcaca ttgtcacatc agcacatcat agagggtatt
     2161 tacagcgacg tgcagccctt tttcgggcgg ggcaaagtcg ccgattacat ccctgcgttg
     2221 gcgcgggttc gccccgatca gttgggtatt gccctgcaaa ctttagacgg gcagaccgcc
     2281 gttgccggag attcggaaat ccgtttttcc atccaaagta tttccaaaac tttcacctta
     2341 gcgctggctt ttgccttgga aggcaacgac ttatggaaac gcgtgggcgt ggagccatcg
     2401 ggtaatccgt tcaattcttt ggtgcaattg gagtttgaaa acggaaagcc gcgcaatccc
     2461 ttcatcaacg ccggagcgct ggtcgtgacg gatgtgctgt gcaggcatta cgacgaccct
     2521 aaatcggtga ttttgaacct catccgcagt ctcgcgggta gtgacgacat cagtttcgac
     2581 gaagaagtag ccgcttctga aaaggccacc ggcttccgca atcagtcctt ggcttggttc
     2641 ctcaagtcct gcggcaacct ccactccgat gtagatacgg tattagacgt gtattttcat
     2701 caatgctcta ttgccatgaa ctgccgcgag ttgagtcgag ccttcctgtt tttggccaat
     2761 cacggcgtac acccctacac cggcgaggcg atgctgaccc gcagtcaggc caagcgcctc
     2821 aatgccatcc tgctcacttg cggattttac gatcaggccg gcgagtttgc ctttcgggtg
     2881 ggtatgccgg gcaaaagcgg cgtgggcggc ggcgtcgcgg ctttcattcc cggtgaattg
     2941 gctgtagcgg tgtggagtcc tgaattgaac gaacacggta actcggtggt gggcatgcag
     3001 gtgctggaat ggtttactac gcggacaggg aggtctattt tttgatattc agcgtcacac
     3061 aataagctgt tagccataag ctgcaaccaa gctctgtgca ttggtttttc caaagtagaa
     3121 aaattcattt tcaaagacac attttgaaat ttcccaaacg gaaaatctcg tttcttatcc
     3181 caaaacaagc taaaagctaa aagctaacag attatagttt tgggaggtat atagaattgt
     3241 gacaatttac ctaccttagc cacctcaacc acatatcatg aaaatacgca gcgtaagcac
     3301 ctggaaagaa aaccttgact tatccaaacc atacaccatc gcctacaaaa ccattgacca
     3361 tgtagaaaac ctcttcgtgg tcgtcgagtt ggaaaacggc atcatcggcg taggttccgg
     3421 ctctcctgcc gaatttgtca ccggagaaaa catccacgct tccgcagagg cactgtccgg
     3481 cctggtagaa ggactgctgc tgaagcaaga catccgccac tacaagcgcc tcattcgcga
     3541 tgccgctgcg caattgcccg cccgtcctgc cgcattggcc gccattgaca tcgccctgca
     3601 cgatgctttc gcacaattgc tgggcctccc cttggccgat tacctgggcc gggtgtatca
     3661 gggatttccc acgtccgtca ccatcggcat tcaatcggta gagcaaacgc tggaagacgc
     3721 ccgtgctttt gtagccgacg gtttccgggt catcaaactc aaaaccggcg tctcggctga
     3781 acaggacatc gagaccttca gccgcttgcg cgagaccgtc gggccggaca tcaccatccg
     3841 cgtggatgtg aatcaggggt acaaaacttc tgatttgctg atatttgatt atgctgtccg
     3901 ggatctgaat gtagagtttt acgaacagcc gctgccgccc aacaacctcg acgagatgtt
     3961 gttgctaccg cccgatttgt gcgccttctg cgctgccgac gaagacctgc tcaccatgga
     4021 agatgccgtg caaatggccc atcacccgca gccctatggc atttacaaca tcaaactgat
     4081 gaaatgcggc ggcgtggatc atggcctcaa aatagccgaa gtggccgaaa tggcgggcat
     4141 ccacctgatg tggggctgca acgacgagag cgtggtgagc atctcggcgg ccctccatgc
     4201 agctttggcc agtaccaaca cccgctacat tgaccttgac ggcagctttg atctggcccg
     4261 cgacatcgct cggggcggtt ttgtgctccg cgacggttgt ctttatgaca atgggaaacc
     4321 gggtctggga gcggaacttt tagctgcaag cctttagcca caagctgcaa gccggattca
     4381 gatagcgccg cggcgcgctt gcgatacaaa gcttgcggct tgaagccaac agcttgcagc
     4441 catagtttac attaaaaaag aaaggagatc atggacatat tggccgaaat ccgcgaggag
     4501 cgccgtcgtc aggatgccaa atggggcgag caaaaccaca gtcccatcga gtggagcgcc
     4561 attctcaccg aagaagtggg cgaggtatcc aaagaggcgc tggaattgca ttttgcgcag
     4621 cttcaaaata cggacaaatc ggaagactgg tatgccgaaa aattggccgc ttatcgcaag
     4681 gaagtcattc aggtggctgc cgtggcgctg gcgatggccg agagtttgga aaggaataag
     4741 tggaggacgc tgcggcgggg cgaaagtcag tgaagatgaa aaaagagttt tgatggcggg
     4801 tagaacccgc aggataggtc gcacgagatg gaatctcgcg cgagcggaaa gaaataagtg
     4861 gagggcgttg cagcggggcg aaagtcagtg aacatgaaat agatgccggc gcgaggttct
     4921 accttcatgt ttaccacgca cggcgtggtg tcgaatatct tgcgggnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnctccccaa cgccacccag cgccgccgcg gtttccatca gccgcacaca
     5101 ttcctggcgg tagcctttct cgtcatcgcc gcgcgctttt ttggccaact ggatacactg
     5161 ctcgaaagtg gctttgcctt tgaactccga attgcgcagc agcataccaa actccgccac
     5221 ggccgccgac caacggaaat tatcgctgct cttcgctgcg ggtacag
//
LOCUS       DAORLF010000344         5249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8600_length_5249_cov_2.032730, whole genome shotgun sequence.
ACCESSION   DAORLF010000344 DAORLF010000000
VERSION     DAORLF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_5249_cov_2.032730"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..886
                     /locus_tag="PK198_20900"
     CDS             101..886
                     /locus_tag="PK198_20900"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF41269.1"
                     /translation="MELQILSTTLLPEYLLRVPKGLQTALEALQDAPLSTPGFSFYIS
                     VASVFSSKIEGEPIELDSYIKHKRDGAPFQPDYTKKIDDLYEACLYAQNHPLHEENVR
                     QAHRLLSKHIVADAWQGRYRAQNMYVTTDDGRIEYVAALPSQVPGAMERLYQDIDLLL
                     AQALSIEEVFYFAAMIHLVFVKIHPWNDGNGRCARLLEKWFLAKKLGEKAWFIQSEKM
                     YYQHHSSYYNNIRALGLEYQELDYGKALPFLLMLPGALIVDLG"
     gene            complement(993..1772)
                     /locus_tag="PK198_20905"
     CDS             complement(993..1772)
                     /locus_tag="PK198_20905"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HRF41270.1"
                     /translation="MKKWFFAFALLSISTLAFQACEKEQNLNDEALIEAIVAAELTAI
                     TPSELPAEARQTVEEMFFDTYMEDIYRAKNLGYKVELGNEGCLYFNTDGEMLEFRDDP
                     TGRGPRGGGFGPNGPHGPCFNRLLGFGRPVRPANLPESITSYITENYPDATIRIARTN
                     SERTIVLISGPVVLLFDADGNFIREVDPLENCNPVRCNPVQGSALPEAIRQYIADNYP
                     DAVFRNACVRHDRVVVFMMNDDARLILVFDRATGNFLFSRP"
     gene            complement(2372..3919)
                     /locus_tag="PK198_20910"
     CDS             complement(2372..3919)
                     /locus_tag="PK198_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41271.1"
                     /translation="MMENIYHNDSDSVENNIRRSVREWEEAPPAGWDTPSPALWDKIA
                     AATPQPPAATGLIWWKWLSGALLVLGVVAGSLWWTDKPSTPKQQVPTTAQTPLMAEKY
                     KQPAADPQPESDQQGLPETPAAASVQGNELPKRTSSAVHISTRKTPASSIRNAAFGER
                     EGLSKTDNLIQNEEDANAGLPTQNEATTAELPVPASETSLSAALPETQDGAGTPLAEV
                     PALVLWSPTAPLESAATELLALPAPSVEMAAHSIKRAPKRGHFYAGVMAAPNETFRRI
                     QSDRPIAQLPAFLKENESATWATEYGVLVGWQPNRRFALSMGVGSYNMSMKTQHRFRV
                     PFDPGRERPIGNNNFESSYNLSVPSSYGDANVEVAVQRPGNQPLIPGQFLTVDTRTQT
                     ELRYTNIPLTATYFLHSSPRLSIGIKGGVALSLLDRQDFSATSTISMRGLRTRNITVQ
                     RQAPDIQKTMVDYHLGAALWYRPAPGWALCIEPSYRQAAGAAIEYEAYSVSQYAWGVQ
                     LGVQKSF"
     gene            complement(3928..4467)
                     /locus_tag="PK198_20915"
     CDS             complement(3928..4467)
                     /locus_tag="PK198_20915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF41272.1"
                     /translation="MDEKEKQLLADCLRHKADAQRQLYERYKVQMYRLCLRYAGNSAE
                     AEDLLQEGFIRVFSDLHQFRGEGALGGWIRQVVLRTALQYLRKRMKEAPMVSEDLIPD
                     SLAMATFPDEDWDARQIVHLLQQLPEGYRTVFNLFVIEEYSHKEIADMLQISESTSKT
                     QLFKAKAHLRRHLEKASVN"
     gene            4734..>5249
                     /locus_tag="PK198_20920"
     CDS             4734..>5249
                     /locus_tag="PK198_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41273.1"
                     /translation="MQLTAGFLPFTAGFCLFTSKSSENLEKGMRPLYLYRRGRPHSAR
                     KHHKIKFIYHANSKPLTMKKNFSFLRAAFTGLSLLLGTSYLFAGNTIPLGALLDGYGK
                     IANPQNLTGEVDFSGFYPVMDECEGLIFLPPPVAPGWNALGTGLGAIVRAIAISGSDV
                     YVGGNFTDAGGN"
BASE COUNT         1222 a         1309 c         1413 g         1305 t
ORIGIN      
        1 tctaatcctt tggcctaaag aaaatgagca tttatttttg ccccactact aataccgacc
       61 tggtaaacaa gcattttaac tcagtacatc cacttttatc atggagctac agattctttc
      121 taccacttta ctgcccgaat acctgcttcg ggtcccgaaa ggcttacaga ccgcattgga
      181 ggccttgcag gatgcgccgc tttctacccc cggattcagc ttttacatct ctgtggcttc
      241 cgttttcagc agcaagatcg aaggagaacc gatagagttg gatagctata tcaaacacaa
      301 aagagacggg gcgccctttc agccggatta caccaaaaaa attgatgatt tgtacgaagc
      361 ctgcctgtac gctcaaaacc atccgctcca tgaagaaaat gtgaggcagg cacatcggct
      421 tttgagcaaa catattgtgg ccgatgcctg gcagggccga taccgggcgc agaatatgta
      481 tgtgacaacg gatgacggca gaatcgagta cgtcgctgca ttgccctcgc aggtgccggg
      541 cgctatggag aggttgtatc aggatataga ccttttgctg gcacaagcac tgagcataga
      601 agaagtgttc tattttgctg cgatgattca cctcgttttt gtaaaaattc acccctggaa
      661 cgacggaaac ggcaggtgtg ccagactgct tgagaagtgg tttctggcaa aaaaactcgg
      721 agaaaaggct tggtttatac aaagcgaaaa aatgtattac cagcaccact caagctatta
      781 caacaacatc cgggcattag gcttggagta tcaggaactt gattacggaa aggcgctgcc
      841 ttttttgctc atgttgccgg gggcattgat cgttgacctt gggtagtagt gccaaaaaac
      901 aaggaccgcc tcgacacttc tgtcaaaacg gcccttgctg atgaaataaa atcacacctg
      961 gaatacaggg cagtcagcgc cccgaatcca ttttatggac gagaaaaaag gaaattgccg
     1021 gtagcgcgat caaacactaa gatcaatcgg gcatcgtcgt tcatcataaa tactacgacc
     1081 ctgtcatggc gcacacaggc gttgcggaaa acagcatcgg gatagttgtc ggctatgtac
     1141 tgacgaatgg cttctggcaa ggcagaaccc tgtacgggat tgcaacgcac cggattgcag
     1201 ttttccagcg ggtccacttc gcggataaaa ttgccgtcgg catcaaacaa cagcactacc
     1261 gggccgctga tgagtactat ggtgcgctca gaattggtgc gggctatgcg aatggtggca
     1321 tccgggtagt tttcggtgat gtagctcgtg atggattcgg gcaggttggc cgggcgtacc
     1381 gggcgaccaa agccgagcag gcggttgaag caggggccgt gcgggccatt ggggccgaaa
     1441 ccacctccac gcgggccacg tccggtcgga tcatcgcgga attccagcat ctcgccgtct
     1501 gtgttgaaat acaggcagcc ttcgttgccg agttcaactt tgtagccgag gtttttggcg
     1561 cggtagatgt cttccatata ggtatcgaag aacatttcct ctaccgtttg gcgggcctcg
     1621 gcgggcaatt cggacggcgt gatggcggtc agttcggcag caacgatggc ctcaatgagg
     1681 gcctcatcgt tgaggttttg ctctttttca caagcctgaa aagccagggt ggaaatagag
     1741 agcagggcga atgcaaaaaa ccattttttc atttggaacg tgctttttaa gtgaatgaat
     1801 tttgtgaaca atctatccta atggtgccaa ggcggggggc aaagttgcct gagggctatt
     1861 tttttttatt caacctgaaa aatattgaat tgtaaatcga aataacgccg ggtaacgctt
     1921 gctgctactt gataaagcgc aaaattgcct gtacttttgc aaaaaaggca agttcggaag
     1981 cgcaagaagc ctgggatgag gcggggtatt cggaagacct tttgcttcaa gagcatatta
     2041 gaactccata caaaagctcc tcgttactat gaaagtggtg cttgacacaa atattttggt
     2101 agtctccatc cctttacaca ctcgttatta tcctattttc gatgctttcc tcaatgagca
     2161 tttttccctt tgtgtcacta ctgatatttt agcggagtat gccgagattc tacatcaaaa
     2221 gtacatactt cgacctttgg tggccgagaa cgtccttaaa accattgaga actccccaga
     2281 tgtaatcatg ctccataaat gccagtcaaa gtgatctcgg cagatgactt tctgaagatg
     2341 gtttcctctt tatagcggca ctgtgtccat ctcaaaaact cttctgcacc cccaactgca
     2401 cgccccaggc atactgactc acactgtaag cctcatactc tatcgctgcg cccgcggcct
     2461 gccgatagga aggctctatg cacaatgccc aacccggcgc cgggcgatac caaagggctg
     2521 cccccaagtg ataatcaacc atcgtttttt gaatatcggg agcctggcgc tgcacggtga
     2581 tgttgcgtgt gcgcaagcct ctcatgctga tggtggaagt ggccgaaaag tcttgcctgt
     2641 cgagcaaact gagggccacg ccgcctttga taccgatgga aagccgggga gaactgtgca
     2701 ggaagtaagt ggccgtcagc ggtatattgg tgtagcgaag ttctgtttgt gtacgcgtgt
     2761 ccaccgtcaa aaactgcccg ggtataagcg gctgatttcc ggggcgttgc accgctactt
     2821 ctacgttggc atcgccatag gatgagggca ccgacaggtt gtagctgctc tcgaagttgt
     2881 tgttgccgat ggggcgttca cggccgggat cgaaaggaac gcgaaaacgg tgctgtgttt
     2941 tcatgctcat gttatagctg cccacaccca tactgagcgc aaagcggcgg ttgggctgcc
     3001 atcccacgag cacgccgtac tcggtggccc aggtggcaga ttcgttctct ttgaggaaag
     3061 ccggcaattg tgcaatcggc cggtcagact gtatcctgcg gaaggtttcg ttgggagctg
     3121 ccatgacgcc cgcataaaaa tgaccgcgtt tgggtgcgcg cttaatggag tgcgccgcca
     3181 tctctacaga tggtgcaggc agagccaata attctgtagc agccgattcg agcggcgctg
     3241 tgggcgacca caaaacgagc gccgggactt cggcaagggg cgttcctgcg ccatcttgcg
     3301 tttcgggaag cgctgcggac agtgaagttt ctgaagcagg tacgggaagt tcggccgtgg
     3361 tcgcctcatt ttgcgtagga aggccggcat tggcatcctc ctcattttgt atcagattgt
     3421 ctgtttttga caagccctcc ctttcgccga atgctgcatt gcggatggat gaagcaggcg
     3481 ttttgcgagt tgaaatatgg acagcagaag acgtacgctt gggaagctcg ttgccctgca
     3541 cagaagcagc ggcaggcgtt tccggcaagc cttgctgatc ggattcaggc tgtggatcgg
     3601 cagccggttg tttgtatttt tcggccatca ggggcgtttg tgctgtcgtg gggacttgtt
     3661 gtttaggagt tgatggcttg tctgtccacc acaaactgcc tgcaacgact cccaaaacga
     3721 gcaacgcacc actcagccat ttccaccaga tcagaccggt agcggcaggc ggctgcggag
     3781 tagcggcggc aattttatcc cacagcgcag gcgagggcgt atcccagccg gcgggaggcg
     3841 cttcttccca ttcgcggacg gagcggcgga tgttgttttc aacagaatca gaatcgttgt
     3901 ggtatatatt ttccatcata ttttcattca gttcacagat gctttttcca agtgccgccg
     3961 caagtgcgct ttggctttaa ataattgggt tttggaggtg ctttcgctta tttgcagcat
     4021 gtcggcaatt tctttgtgcg agtattcttc gataacaaag aggttgaata ccgtgcggta
     4081 gccttcgggc agttgttgca acaaatgcac tatctggcgg gcgtcccagt cttcatctgg
     4141 aaaagtggcc atcgccagac tatctggtat caaatcttcc gacaccatcg gcgcttcttt
     4201 catgcgcttc cgcaaatact gcagggccgt gcgcaacacg acctgccgaa tccagccgcc
     4261 cagcgcgccc tcgcccctga actggtgcaa gtcggaaaac acgcgaatga acccttcctg
     4321 caacaaatct tccgcctcgg cgctgttgcc ggcatagcgc aaacacaagc gatacatttg
     4381 tactttgtaa cgctcgtata gctgtcgttg ggcatctgct ttgtgcctca gacagtctgc
     4441 cagcaattgt ttttcctttt cgtccacgtg tgtgcagtag ctgtgctgaa tatgatggtg
     4501 caaaatacgg acttgcggtt gcctgcgagt gaagtttttt ttgggaaagc cggaaccggt
     4561 aggcggtttg ccgtcggcag tcggcggttc taagcgcgag ggaactaccg gtatgcggtg
     4621 tgcagtcggc ggttctaagc gcgagggaac taccggtatg cggtgcgcga cttgcagctt
     4681 gtagccagca gcttgaggcc atcagccggc agccgttcca acccgaaatt cccatgcagc
     4741 tcaccgcagg atttttacca ttcaccgcag gattttgtct gttcacctca aagtcgtctg
     4801 aaaatttgga gaaggggatg cggccactgt acctttaccg ccgtggaagg ccgcacagcg
     4861 cccgaaaaca ccataaaatc aaattcattt atcacgctaa tagcaaacct ttaaccatga
     4921 aaaaaaactt ttcattccta cgcgctgcct tcacaggctt atccctttta ttggggacat
     4981 cttacctttt tgccggcaac accatcccat tgggagcctt gctggatggt tatggtaaga
     5041 ttgccaaccc acaaaacctg acaggggagg tggacttctc aggcttctat cctgtgatgg
     5101 atgaatgcga aggcttgatt ttcctgccgc cgcctgttgc tccgggttgg aacgcgctgg
     5161 gtacaggctt gggcgctatc gtccgtgcta ttgccatctc tggcagcgat gtgtatgtag
     5221 gtggaaactt taccgatgcc ggcggaaat
//
LOCUS       DAORLF010000345         5227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8652_length_5227_cov_1.718484, whole genome shotgun sequence.
ACCESSION   DAORLF010000345 DAORLF010000000
VERSION     DAORLF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8652_length_5227_cov_1.718484"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    181..274
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <275..>554
                     /locus_tag="PK198_20925"
     CDS             <275..>554
                     /locus_tag="PK198_20925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41274.1"
                     /translation="TDEKGEALIGASVLVKGTTVGTVTDVDGSFQLTVPVGSTTLIIS
                     YTGFQSQDVTLGASSVMDVTLLEGLILEEAVVTAFGVRKDKSNLGYAVS"
     assembly_gap    555..664
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <665..>1633
                     /locus_tag="PK198_20930"
     CDS             <665..>1633
                     /locus_tag="PK198_20930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF41275.1"
                     /translation="SGSSIIIRGFTSFTGSNQPLVVVDGVPIDNSGGGTALQTGPVSS
                     NRAIDLNQEDIESISVLKGAGATALYGSRAAGGVLLITTKKGRVNQKNAITYTANYAA
                     QEVNRIPDYQNEYGQGAAYNPAAISSWGPRIQGQNVILPVDYRGGLISPNDSVALTAY
                     PNNVSDIFRTGSNMQHNLAFQGGTEKSAYRLSLGYLDDQGVLENNRLQRYNFGANASS
                     NITTKLTAAISANYVNNTSQRTQQGNQLSNPLFRAWFLPRSLNLSGLPFESADGLRQL
                     NYDPAVDNPYWTIANNLYDDEINRFFGNVSLRYQLLSWLSAEYKLGV"
     assembly_gap    1634..1673
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1674..>2814
                     /locus_tag="PK198_20935"
     CDS             <1674..>2814
                     /locus_tag="PK198_20935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41276.1"
                     /translation="IGARGGANTAANTAGGILEGSDLSRIMNSFLTLTGNRQLGSDVD
                     LTFVLGQETSDNQFRSSQVIGRGVTVRNFRDLDANTTTFVPSFGRSQSRLIGLFGNVT
                     AVYRNFASLDLSVRNDWSSTLPKGKNSYLYYSAAGTFNLTQAIPSLKSDFVNLLKVRA
                     NYGRIGRASLLYATESYYSGFNPADGFGPLIQFPFNSLPGFSLNNTAGNPDLEPEFTT
                     TVEAGIDATLWKGRIALELTRYRQSTSGIILSVPNSPAAGISAIVKNAGDLETNGWEL
                     GLTVTPVKTSKGSWSTNFNFTQFRSIVKSLAPGVQNIFLGGFTTPNIRLVEGDEYGQI
                     YGNAYKRDANGNLLLTATGLPQPTANVEKLGNPNPRWTLGVNNNIS"
     assembly_gap    2815..2850
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2851..3351
                     /locus_tag="PK198_20940"
     CDS             <2851..3351
                     /locus_tag="PK198_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41277.1"
                     /translation="IRQGGDIYSRNIADLQRNGATIETAEFARFNADGTVATPYRFDG
                     IFETGPNAGKANEGEAERRVTAEQYWGNAGKYVAAEGFIYDVSWFRIREAGLSYTFPK
                     KMLEKTPFGGLEIGVFGRNLFLHAPNYPHLDPEQNVLGISNAQGLEFNALPQMRSYGV
                     NLRVTL"
     gene            3429..>4618
                     /locus_tag="PK198_20945"
     CDS             3429..>4618
                     /locus_tag="PK198_20945"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF41278.1"
                     /translation="MKLGKLIRYSVLILGLSLYSTSCKDYLDVNLDPNAVQDAPIEQL
                     LTTCTVSLGFFGGSDLHRYSSLLAQQFSGQGQGATTQPQEYDRYNIQGSDLNNVWNLL
                     YASILSDLELVIQKADTEKSPHYAGVAKIMKAYVFHLMVDAWGKVPYSEGLKFTANTA
                     PKFDDGEAIYTDLERLLDEAIADLNAPSIKSPAANSTIFPGTFTASRPRWVRLANTIK
                     LRLYLHQSKKSRANTVQKITALVNSASVSFMESNADNFQMAFINEARRQNPIHQFELD
                     RVNQFFPGATLVNLMNQRNDPRRPRYFTPFPFTRDAAPAYAGAKPGDAPSFRYSRMHT
                     YLRGDTTNTTPVAAQADGSITNSAYTYNGTAPIRMLTFAEYNFIRAEAAVYGAPGTAE
                     TFFQA"
     assembly_gap    4619..4640
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4641..4961
                     /locus_tag="PK198_20950"
     CDS             <4641..4961
                     /locus_tag="PK198_20950"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF41279.1"
                     /translation="RASMSGAGVPTAQIDDYIAANGTLTGTEAERVRRIIEEKYIANY
                     GVTMESWTDWRRTGFPQITAPVNALTPFTPRSLFYPQTEIDLNPNAPAQKTDMSERVF
                     WDVQ"
BASE COUNT         1166 a         1382 c         1196 g         1181 t
ORIGIN      
        1 atttacttaa catagcgggc attcggaacg acggtttcgg atgcccgttt tttatatccc
       61 gaatctcgaa attcgtgaaa acatagcact ctcaggcagc attagtcccc ccttgcgcga
      121 tatatacaga ccgcggctgt atcagccatt gatttttgcc ccactactaa ggattttcta
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngactga cgaaaaaggg gaagccctca
      301 tcggcgcctc cgtactggtg aagggtacca ctgtgggtac agtcactgat gtggacggga
      361 gcttccagct aacggtaccc gtaggctcta ccaccctgat catcagctac acaggctttc
      421 aaagtcagga tgtaactctg ggtgcttcca gtgtaatgga tgtcacgctt ctggagggtc
      481 ttattctcga agaggccgtg gtgacggctt tcggagtgag aaaggacaag agcaatctgg
      541 ggtatgcggt ttccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnctcggg atcgagtatc atcatccggg gttttacctc ctttaccggc agcaaccagc
      721 ccttggtagt agtggatggt gttcctattg acaacagcgg cgggggcacg gccctgcaaa
      781 ccggcccggt ttcttccaac cgcgctattg acctcaatca ggaagacatt gaaagcatca
      841 gcgtactcaa aggcgccggc gctaccgccc tgtatggttc gcgggcagca ggcggtgtgt
      901 tgctcatcac gaccaaaaaa ggccgtgtga atcagaaaaa tgcgataacc tacaccgcca
      961 actatgcagc tcaggaggtc aaccggatac cggactacca aaatgagtac ggtcaaggcg
     1021 cagcatataa cccggcagcc atttcctcct gggggccgcg cattcagggg caaaacgtga
     1081 tcctgcctgt tgattatcgc ggtgggctta tttctcccaa cgactcggta gcgctcactg
     1141 catacccgaa caatgtaagc gacatattcc gcacaggctc caacatgcaa cacaacctgg
     1201 ctttccaggg cggcaccgaa aagtcggctt atcgtttgtc tttgggctat ctcgacgatc
     1261 agggcgtatt ggaaaacaac cgcctgcagc gctataattt tggcgcaaat gcttcttcga
     1321 acattacgac caaactgacg gcggccattt ctgcaaatta tgtcaataat acctcccaac
     1381 gcacccagca gggtaaccaa ttgtcgaacc cgctgttccg cgcctggttc ctgccgcgca
     1441 gcctcaatct cagcggcctg cctttcgaat cggccgacgg gcttcgccaa ctcaactacg
     1501 acccggctgt ggacaatcca tactggacca tcgcaaacaa cctgtacgac gacgaaatca
     1561 accgcttctt cggcaacgta agcctgaggt atcaattgct gagctggctg agtgctgaat
     1621 acaagcttgg cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcggcg
     1681 ccagaggagg agccaacacg gctgccaata cagccggcgg tatccttgag ggcagtgacc
     1741 tttcccgcat tatgaactct ttcctcaccc ttacgggcaa ccgccaactc ggctccgacg
     1801 ttgatctgac ttttgtactc ggtcaggaaa ccagcgacaa tcaattccgt agttctcagg
     1861 tcattggccg gggggttacg gtgcgtaatt tccgcgattt ggatgccaat accaccacct
     1921 ttgtgccgtc cttcggtcgc agccaatccc gtttgattgg tttattcggc aatgtcacgg
     1981 cggtttaccg gaattttgca tcgttggacc tgtctgtgcg caacgactgg agttctacct
     2041 tgcctaaagg gaaaaacagc tatctgtact actctgctgc cggcaccttc aatctgacac
     2101 aggcgattcc ttcacttaag tcagacttcg tgaaccttct caaggtacgc gccaattatg
     2161 gccgtattgg tcgtgccagc ctcttgtacg ctactgaatc gtattattcc gggttcaacc
     2221 cggcggatgg ttttggtccg cttatccaat tcccattcaa cagcctgcca gggttctcgc
     2281 ttaacaacac ggccggcaac ccggacctgg agcctgagtt tactaccacg gtggaagcag
     2341 gtatagatgc gacgctgtgg aagggccgaa tagcgctgga attgacacgc tatcgtcaaa
     2401 gtacctccgg tattattctt tctgtgccca attctccggc tgcaggtatt tccgctatcg
     2461 tgaaaaatgc cggcgatctg gaaaccaacg gatgggaact ggggcttacg gtgacgcctg
     2521 tgaagacgag caagggatca tggtcaacca acttcaactt tacgcaattc aggtctatcg
     2581 tgaagagtct ggcgcccggc gtacagaata tcttcctcgg cggttttaca acgcccaata
     2641 tccgcttggt agaaggtgac gaatacggcc agatttatgg taatgcctac aagcgcgacg
     2701 ccaacggcaa cctgctgctc actgctaccg gattgccaca gcctaccgcc aacgtggaaa
     2761 aactcggcaa cccgaacccg cgctggacgc ttggcgtgaa caacaacatt tcttnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atccgccaag gcggcgatat ctactcccgc
     2881 aacattgccg acttgcagcg caacggcgcc accatcgaaa cagcggaatt tgcccgcttc
     2941 aacgcagatg gtacggtagc cacgccgtac agattcgacg gtatcttcga gacaggtccg
     3001 aacgcaggca aagccaatga aggcgaggca gaaagacgcg ttacggctga acagtactgg
     3061 ggtaatgcag gcaaatatgt tgccgctgaa gggtttattt atgatgtatc ctggttccgt
     3121 atccgcgaag ccggactcag ctatacgttc ccgaaaaaga tgctggaaaa aacgcctttc
     3181 ggaggtttgg agattggcgt atttggccgc aaccttttcc tgcacgcgcc gaactacccg
     3241 cacctcgatc cggagcaaaa cgtactcggt atcagcaatg cacagggctt ggaattcaac
     3301 gccctgccgc aaatgcgttc ctatggcgtc aacctgcggg taacgcttta aaatctggtt
     3361 tcccggcgtc cgattctcct ggaatccggc gtcgaaactc atttttctaa cttataaaac
     3421 agaaattcat gaaacttgga aaactcattc gatacagcgt tcttattctt gggctttctt
     3481 tgtacagcac ttcgtgcaaa gattatctgg atgtgaacct tgatcccaat gcagtgcagg
     3541 atgctcctat tgagcaattg cttaccactt gcactgtttc tcttggcttt ttcggtggtt
     3601 ctgacctcca ccgctatagt tctttgttgg cacaacagtt ttccggccag ggccagggtg
     3661 caaccaccca accgcaggaa tatgatcgtt acaatattca gggttccgac ctcaacaatg
     3721 tgtggaactt actgtatgct tccattttga gcgatctgga attggtcatt caaaaagccg
     3781 acaccgaaaa aagtcctcac tatgccggcg tagccaaaat aatgaaggct tatgtcttcc
     3841 atttgatggt agatgcctgg ggcaaagtgc cttattcaga aggcttgaag tttacagcga
     3901 atacggcgcc caaatttgac gacggcgagg ccatctacac agatctggag cgcttgcttg
     3961 acgaagctat tgccgacctg aatgcgccct ccatcaagtc gccggctgcc aactccacca
     4021 ttttcccggg tactttcacc gcttcccgcc cacgctgggt gcgcctggcc aatacgatca
     4081 aactcaggct ttatctgcac caaagtaaga agagtcgtgc taataccgtg cagaaaatca
     4141 ccgcattggt caacagtgca agcgtttctt tcatggagtc caatgccgat aacttccaga
     4201 tggcgttcat caatgaagct cgccggcaaa acccgattca tcagttcgaa ttggaccgtg
     4261 tcaatcaatt cttcccgggc gccactttgg tcaacctgat gaaccagcgc aacgacccgc
     4321 gccgtccacg ctactttacg ccattcccgt tcacccggga tgctgctccc gcctatgcag
     4381 gcgctaagcc tggtgatgca ccctctttcc gctactcccg tatgcatact tacctgcgcg
     4441 gcgataccac caatactacg ccggtggcgg ctcaagccga tggctctatc accaatagcg
     4501 cttatacata caatggtaca gcgcccattc gcatgctgac ctttgccgag tacaacttca
     4561 ttcgtgccga agccgctgtt tacggcgctc ccggaaccgc tgaaaccttc ttccaggcnn
     4621 nnnnnnnnnn nnnnnnnnnn cgcgcttcca tgtcgggtgc aggcgtgcca accgcacaaa
     4681 ttgacgacta catcgccgcc aatggaacgc tgaccggtac agaggctgag agagtgcgca
     4741 gaattatcga agagaagtac attgccaact atggcgtcac tatggaatca tggaccgact
     4801 ggcgtcgtac gggcttcccc caaatcactg cgccggtgaa tgccctcacg ccttttactc
     4861 ctcgctctct gttctatcct caaacggaaa ttgacctgaa tccgaatgcg cccgctcaga
     4921 aaaccgacat gagcgaacgc gtattctggg atgttcaata agcctatttt ttgattcaca
     4981 cgaagagccg cctcgcacgc cggggcggct ctttcttttt gcgctttttt tgaaaaatct
     5041 ctgttatctt tgcctatctg tttgaaaatg caaccatgat aacatggaag acccaattct
     5101 gcgcaaagca caaaagcgcg tcaaatccaa aaagggattt tacacccatc ttgcgtcttt
     5161 tgtgtcggtg ggcattttct ttttgttgct caacctcgtt acctatgacg gcgaaatgtg
     5221 gtttttc
//
LOCUS       DAORLF010000346         5220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8673_length_5220_cov_2.358374, whole genome shotgun sequence.
ACCESSION   DAORLF010000346 DAORLF010000000
VERSION     DAORLF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8673_length_5220_cov_2.358374"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK198_20955"
     CDS             <1..514
                     /locus_tag="PK198_20955"
                     /inference="COORDINATES: protein motif:HMM:NF027664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HRF41280.1"
                     /translation="EAYFKVTRDEQRPFRVQAAYTLTEVLGTTFNVRAWPGENAVEVT
                     VETGRVRFSPVSQPGESLTLHPGERGQCLTNGNLVKSGDMEINALSWMTGQLRFRRAK
                     LSEALQAIGRHYEVVLDLEKTALGDCSYTGSFEKAELKDVLLTLELNYGVRISEVSPK
                     RYVLRGGSCR"
     gene            739..3273
                     /gene="lon"
                     /locus_tag="PK198_20960"
     CDS             739..3273
                     /gene="lon"
                     /locus_tag="PK198_20960"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRF41281.1"
                     /translation="MEIALYEVLISMGALLSYRQDSSGFHLLRDAFRKQFNKYRNKAM
                     FEEILAHRGIEEEGDFLPMISIEDEEEPVNGEGIPEVLPVLALKNTVLFPGIVIPITV
                     GRNKSIKAINTAYEENRLIAVLSQKDANIEDPAAEDLYRVGSVARILKLLKMPDGTTT
                     AILQGRTRFSMEEMLGEHPLMTARVRTLGYVQASNVLEYEALISSILDLAKDIIELSP
                     NIPSEAAVMLRNISNHSFLLNFVSSNLNAKIQLKQQLLETDDLREKASLILQQLHAEL
                     QLLELKDQIESKVRGDIEKQQRDYFLSQQLKTIQEELGQNPQEEDLQNLVARAEKKRW
                     GKGAADVFQRELNKIRRMNPQVAEYSIQLNYLELILDLPWDEFTKDNFNLKKVKEVLD
                     EDHFGLEKVKERILEHLAVLKLKGDMKAPILCLVGPPGVGKTSLGKSVARALKRKFIR
                     MSLGGLHDESEIRGHRKTYIGAMPGRILQSLKRAGSSNPVFILDEIDKVGKDFRGDPS
                     SALLEVLDPEQNSTFYDNYLEMEYDLSKILFIATANSLSTIQPALLDRMEIIEISGYS
                     MEEKVEIAKRHLIPEQRAEHGLEGKHVKLRPEVISRIIQSYTRASGVRALGREMAGVM
                     RHIAKNVAMEEPYNVTVKIDDLPKILGPERYSKDMYKEEKISGVAIGLAWTSVGGEIL
                     FIECSLHKGKGHLILTGNLGDVMKESATTALSFIKAHSESMGISPDLFEQKDVHLHIP
                     EGAIPKDGPSAGITMLTTITSAFTGRPVKPFLAMTGEITLRGRVLPVGGIKEKILAAK
                     RAGMKDIILCRENQKHVAEINTAYIEGLQFHYVDRMEEVLEYALEG"
     gene            complement(3419..3823)
                     /locus_tag="PK198_20965"
     CDS             complement(3419..3823)
                     /locus_tag="PK198_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41282.1"
                     /translation="MKNLLLPAVLTILLGAACTNISDDSLPSNDVSGVITNGDWEVSY
                     FWDKTKDETHKFSGYSFVFENGGVLRSIRQGSTTVGTWVQGSGKLILFAGAADPLEEL
                     NDDWIIVERTETLIRLRDDNDEHVEELHFRKR"
     gene            complement(3911..>4432)
                     /locus_tag="PK198_20970"
     CDS             complement(3911..>4432)
                     /locus_tag="PK198_20970"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRF41283.1"
                     /translation="RPLSSLLGFSDHVNAWLINLLVLAIILVLFFLIGLAVRTEVGRR
                     LFHNLEEQWLKPLPFYTIIRETVQQLFSKDKMPFSKVALVDPFGSGALMTGFVTAELP
                     NGFFTVFVPTAPNPTNGFIFHLPPDKVTMVDAGTEDAMRSVISLGIGSGKLFQNKVSE
                     ASPSAPQNPVSEG"
     assembly_gap    4433..4572
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4649..>5220)
                     /locus_tag="PK198_20975"
     CDS             complement(4649..>5220)
                     /locus_tag="PK198_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41284.1"
                     /translation="RSLGLAADLAAVYADTARKLVFTLVMRNVGTQLSTYRSGNREDL
                     PYDFQAGVSKRLKHIPFRFSVIYHHLNRWNLLYDNPYETEDNNIFDEEPVEPGKASLF
                     VDNLFRHFIFNGELLIGQRESLRLRFGYNHFMRQELSVNNYRSLSGFTFGAGIRISRF
                     RLDYGRNNYHLAGGMNHLSISTNLREFKR"
BASE COUNT         1291 a         1358 c         1372 g         1059 t
ORIGIN      
        1 ggaggcgtat ttcaaagtga cgcgcgacga gcaacggcct ttccgggtgc aggcggctta
       61 caccctgacc gaggtgcttg gcaccacgtt caatgtgagg gcctggccgg gtgaaaatgc
      121 cgtagaagta acggtggaaa caggccgggt gcgtttttct ccggtctcac agccgggcga
      181 gagcctgacg ctccatcccg gcgagcgcgg gcaatgtttg acgaatggta acctcgtcaa
      241 atccggcgac atggaaatca atgccctgtc ttggatgacc ggccaattgc gctttcgcag
      301 agccaagctt tcagaggcgc ttcaggccat cgggcgccat tatgaagtag ttctggactt
      361 ggaaaaaact gcgcttggcg actgttccta caccggttcc tttgaaaaag ccgaactcaa
      421 agacgtgctg ctcacgctgg aactcaatta tggagtgcgc atttccgagg tctcccccaa
      481 gcgatatgtg ctgcgcggcg gttcgtgccg ataaggaacg gggtccgaat aagctacatc
      541 ccaccagaga tcataccgac tctgccccat ctgtcctgta cggccttccc ggtctgtgcg
      601 gcctgacttt ttgtcttatc cacgaccatg tactccgaat gtggaaaaat agtcgcctcc
      661 atggctggaa tacttgctaa tattgcgcct gcaagtgcct tgtaatgctg caacggagca
      721 aaaatgcttt gtttcggcat ggaaattgcc ctttacgagg ttcttatatc aatgggcgcc
      781 ctgctgtcat atcggcagga ttcgtccggg tttcatctgc tccgggacgc attccgaaag
      841 caatttaata aatatcgaaa caaagcaatg tttgaagaaa tattggcaca cagaggcata
      901 gaggaagaag gcgatttcct gcccatgatc tccattgaag acgaagaaga gccggtcaac
      961 ggtgaaggca tcccggaggt attgcccgtt ctggcactta aaaacacagt gctgtttccc
     1021 ggcatcgtca ttcctattac ggtggggcgc aacaagtcca tcaaagccat caatacagct
     1081 tacgaggaaa acaggctcat tgccgtgttg tctcaaaaag atgccaacat agaagaccct
     1141 gccgccgagg acctttacag agtgggttcg gtggcccgca ttctgaagct cctcaaaatg
     1201 cccgacggca ccaccactgc catattgcag ggccgcaccc ggttttcgat ggaggaaatg
     1261 ctcggcgagc acccgctgat gaccgcccgc gttcgcacgc tcggctatgt acaagcatcc
     1321 aatgtacttg agtatgaggc gcttatttcg tctattctcg atctggccaa agatatcata
     1381 gagctttcgc cgaatattcc ttcggaggcg gccgtcatgc tgcgcaacat cagcaaccac
     1441 agcttcctgc tcaattttgt ttcgtcgaat ctcaacgcca aaatccagct caagcaacaa
     1501 ctactggaaa ccgatgacct gcgcgaaaag gcatcgctca ttctgcaaca actgcacgca
     1561 gaactgcaac tgctggaact caaagaccag atagagtcga aggtgcgcgg agatattgaa
     1621 aaacaacaac gcgattattt cctcagtcag caactgaaaa ccatacagga agaactgggg
     1681 caaaacccgc aggaagaaga cctccaaaac ctggtagcac gagccgaaaa gaaacgctgg
     1741 ggcaaaggtg cggcggatgt tttccaacgc gaactcaaca agattcgccg catgaacccg
     1801 caggtggctg agtactccat ccaactcaat tacctcgaac tcatcctcga cttgccctgg
     1861 gatgaattta ccaaagacaa tttcaacctc aagaaggtga aagaggtgtt ggatgaagac
     1921 catttcggct tggaaaaagt gaaggagcgc atcctcgaac acttggctgt gctgaaactg
     1981 aaaggcgaca tgaaagcgcc tattctttgc ctcgtaggcc ctcctggcgt aggcaaaacc
     2041 tcattgggca aatcggtggc gcgcgcgctc aagcgcaaat tcatccgcat gtcgctgggc
     2101 ggcctgcacg atgagtcgga aattcgcggg caccgcaaaa cctacatcgg cgccatgccg
     2161 ggccgcatcc tgcaatcgct caaacgggcg ggctcttcca atccggtttt catcttggac
     2221 gaaattgaca aagtaggcaa ggatttccgc ggcgacccct ccagcgccct gctggaagta
     2281 ctcgacccgg agcaaaactc cactttctac gacaactatc tggagatgga atacgacctc
     2341 tccaaaattc ttttcatcgc caccgccaac tccctcagca ccatacagcc ggccctgctc
     2401 gaccgcatgg agatcatcga gatcagcggc tattcgatgg aggaaaaagt ggaaattgcc
     2461 aaacgccacc tcataccgga acaacgcgcc gaacatggct tagagggcaa gcacgtcaaa
     2521 ctgcgcccgg aggtcatcag ccgcatcata caatcttaca cccgcgcatc gggcgtgcgg
     2581 gcgctcggtc gcgaaatggc cggcgtgatg cgccacattg ccaaaaacgt ggccatggaa
     2641 gaaccctaca acgtgacggt gaagatagac gacctgccca aaatactggg gccggagcgc
     2701 tactcgaaag acatgtacaa ggaggagaag atcagcggcg tggccatcgg cttggcctgg
     2761 acttcggtgg gcggcgagat tcttttcatc gaatgcagtc tgcacaaagg caagggccat
     2821 ctcattctca ccggcaatct gggcgatgtg atgaaggaat cagccactac ggcgctcagt
     2881 ttcatcaaag cgcacagcga aagcatgggt atctcgcccg atctgtttga acaaaaagat
     2941 gtacacctgc acatacccga aggcgccatt cccaaagacg gcccctcggc gggcatcacc
     3001 atgctcacca ccatcacctc cgccttcacg ggcaggccgg tgaagccttt cttagccatg
     3061 acgggcgaaa tcaccctgcg cgggcgagtg ttgccggtgg gcggtatcaa ggagaaaatc
     3121 ttggccgcca aacgcgccgg catgaaagac atcatcctgt gccgcgaaaa ccaaaagcat
     3181 gtagccgaaa tcaacacggc ctacatcgag ggcttgcagt tccattatgt ggaccgcatg
     3241 gaagaggtac tggagtacgc attggagggg tagaggataa aaacgccacg ggcccgatct
     3301 ctttgcgggc atcgggcctg tgtgctgcct ctaattcttc atacaaacaa aagcgaattc
     3361 agaaagcgcg atttgcagat aatcaatcag ttatctattt taaaaaacag cccctcattt
     3421 atctcttccg aaaatgcagt tcctccacat gctcgtcatt gtcgtcgcgg aggcggatga
     3481 gcgtttcggt gcgctccacg atgatccaat cgtcgttgag ttcttcaagc gggtcggcgg
     3541 caccggcgaa gaggatgagt ttgccggagc cttgcaccca agtgcccaca gtcgtactgc
     3601 cctgtcggat ggaacgcaaa acgcccccgt tttcaaaaac aaaagaatag ccgctgaatt
     3661 tgtgggtctc gtctttggtc ttatcccaga agtagctcac ctcccagtcg ccattggtga
     3721 tgacgccgga aacatcgttg gaaggcagcg aatcgtcgga aatgttggtg cacgctgcgc
     3781 cgagcaagat ggtgagcacg gcgggcaaaa gcagattttt catggcagtt acatttgaat
     3841 ggtgaatgaa tgattcaaat gtacgccccc gaaggcgggc attgcaaatg ggatgcaccc
     3901 ggcagggaaa ctacccttcc gaaacgggat tttggggtgc cgaaggagac gcctcgctta
     3961 ctttgttctg aaacaactta ccggaaccta tgcccagact gatgaccgag cgcatggcat
     4021 cttcggtgcc ggcatccacc atcgtcacct tgtcgggcgg caggtgaaaa atgaaaccgt
     4081 tggtgggatt gggtgcggtg ggtacaaata cggtgaaaaa tccattgggc aactccgcgg
     4141 ttacaaaacc ggtcatcaga gcgccggagc cgaaggggtc caccaatgct actttggaaa
     4201 acggcatttt gtctttgctg aaaagctgct gcaccgtttc cctgatgatg gtgtaaaaag
     4261 gcagcggttt gagccattgt tcctctaagt tgtggaacag ccggcggcct acctcggtgc
     4321 gcacggccag cccgatgagg aagaacaaca ccaaaatgat ggccagcacc agcaaattga
     4381 tgagccacgc attgacatga tcggaaaaac cgagcaaact gctcagcggg cgnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nncgttggaa aaaggtcatt gtgtcgtggt gattttgttg ttgttcaaaa
     4621 cacggcaaag gccgggccgg ttgcagtgtc agcgcttgaa ctcccgcaga ttggtagaaa
     4681 tactgaggtg attcatgcct cccgcaaggt ggtagttgtt gcggccatag tccaagcgga
     4741 agcggctgat gcgaatgccc gctccgaagg tgaagcccga caacgagcgg tagttgttta
     4801 cgctcagttc ctgccgcatg aagtggttgt agccaaaacg gaggcgcagc gattcccgtt
     4861 gcccgatgag caattccccg ttgaaaataa aatgccggaa gaggttgtca acaaaaagcg
     4921 aagccttacc aggttctacg ggttcttcat cgaagatatt attgtcttcc gtttcgtaag
     4981 ggttgtcata gagcaggttc cagcggttga ggtggtgata aatgacggaa aaccggaacg
     5041 gaatgtgctt cagccgtttg gaaacccccg cctgaaaatc gtagggcaaa tcttcccggt
     5101 tgccgctgcg gtaggtactg agttgggtac ccacattgcg catcaccaag gtaaagacca
     5161 atttccgggc ggtatcggca tacacggctg ccagatcggc ggccaggccc agcgagcggt
//
LOCUS       DAORLF010000347         5215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8685_length_5215_cov_2.127132, whole genome shotgun sequence.
ACCESSION   DAORLF010000347 DAORLF010000000
VERSION     DAORLF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_5215_cov_2.127132"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK198_20980"
     CDS             complement(<1..233)
                     /locus_tag="PK198_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41285.1"
                     /translation="MSLPENITLLLTRQWNGESLTDLEQSARDQWLAAADHAAEADRL
                     HRVWLAADNYPSHYEPDFDAGWNKLKSRMSDSKP"
     gene            complement(230..862)
                     /locus_tag="PK198_20985"
     CDS             complement(230..862)
                     /locus_tag="PK198_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRF41286.1"
                     /translation="MLRSEITDNELMQRLATDGEAAMALIFRRHYEQVCRTVYRVVPQ
                     AETAEDIAQEVFLELWKRRNQLTISTSLGAYLRRAALNRALNYVRDQKMKWSDDAELP
                     AMSDGAPGIPEQLEAKELQKMVDQYIDQLPEKCRLVFVLSRFESLSHAEIAEQLDISP
                     KTVENQITKALRFLRNALGPYIGLLISVFVKICVGASALIGVFGDELPKI"
     gene            1015..2610
                     /locus_tag="PK198_20990"
     CDS             1015..2610
                     /locus_tag="PK198_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRF41287.1"
                     /translation="MSKHNESWGSRVGLVLAMAGNAVGLGNFLRFPVQAVQNGGGAFI
                     IPYLVCFLLMGIPLLYVEWSMGRFGGGRGHHSTPFILDSMAPKQFLWKYIGVFGIFTN
                     VAVAAYYCYLESWTLGYIWHSISGTFKGMDSAQVADFFSGYTAFDDYSNVAFWVFCLA
                     LNTYILSRGLSGGVEKVAKVGMPLLIAFGVLLAIRGITLKSGNAGAVYDGTEGLNYLW
                     TPDFSSIWTPKVWLAAAGQIFFTLSVGMGSIQCYASYLRKRDDVALNAMSAGWMNEFV
                     EVVLGAAIVIPITVGYFGIDGMKDLVQQAGGLGLGFRTLPYLFQQWGPVLAVVAGVRW
                     FGLLFFAGITSSLAMGTPVLGFLRDEFGWKRTSAAWAFGAIVFILGLPTVLFFKYGVF
                     DEYDYWAGTVSLVVFAMFEIILFAWVFGIDKGWEEITRGADMTVPIIFKYIIKFVTPA
                     ILIFVFVGSLPGIWDAITNKAIYEQIVKATDGAVIEELKRKIFFVNISRILLVAVWGF
                     IAFLVYKAYAKRRREGRFTTSEI"
     gene            2684..2842
                     /locus_tag="PK198_20995"
     CDS             2684..2842
                     /locus_tag="PK198_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41288.1"
                     /translation="MNTIALITWLASVITVTGFTGYFFYRVLTNPHAPDVPHSDDVAQ
                     GPKTFDAT"
     assembly_gap    2913..2932
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2933..3898)
                     /locus_tag="PK198_21000"
     CDS             complement(<2933..3898)
                     /locus_tag="PK198_21000"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRF41289.1"
                     /translation="MKKSLMEAAGTLAAARNKTSLDGPLWMSDFRIWGLPLSETSDAE
                     TLCGYLFAAQTPTGFLPSGNRTGAAALPIQGFILEQLRQSSKKSAEFSAPALRTAFHR
                     AIAYHRYLYAHRDPAEEGLLVIEHPEEDGFGNAPGYWYRNSPTLQIQDPFFNTCLAAS
                     NEALMALGHFLKEDISEAAHWYELTVHTMNEKLWDEEAASYRAYDLSTGRFIKVPTLA
                     GLLPMLAEIPDQAQAERMLALLESPAWTDPAYRMYPSCRTSQIASDHCGGWNGAVWPA
                     LNWMLYKGLKRYDFNETAERLRRSTLHLVSEYGFHVAYDPRQQKQG"
     gene            4021..4353
                     /locus_tag="PK198_21005"
     CDS             4021..4353
                     /locus_tag="PK198_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HRF41290.1"
                     /translation="MSKFDEAVEKYSADLKDLGVAFNAELLSAVTKGLGPSIYNQDSS
                     MVACSDAEELARVKTNFLIGKLGLSDGPKLDEALSAVCSQYDKRFKHRAVFYYLLVEK
                     LGKQSVYA"
     gene            complement(4420..>5215)
                     /locus_tag="PK198_21010"
     CDS             complement(4420..>5215)
                     /locus_tag="PK198_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRF41291.1"
                     /translation="SFGVYIAKMNEIGHLDLESRPGKRPGGYNMPLMATGVPFIFMNA
                     TNSFNDLRTLMHESGHAIHSFLTRHYPLKTAKRVPSEVAELAAMTMELLTMEHWDVFF
                     PDQEDLRRAKFAQLEFVLKVLPWIATIDKFQHWLYLNPQHNRAERKAAWMETLLEFSS
                     PEVAPSEVEGYSEYLWHKQLHIFEVPFYYIEYGMAQLGAIAIWKQYRENPDATISNFI
                     NALKLGYTRPIGEIYEAAGIRFDFSRENVRSLGEFVRKELELLMKP"
BASE COUNT         1179 a         1319 c         1365 g         1332 t
ORIGIN      
        1 ggtttagaat cagacatgcg gcttttcagc ttgttccagc cggcgtcaaa gtcaggttca
       61 tagtgtgagg ggtagttgtc ggctgccagc cacacgcggt gcaggcgatc agcttctgcg
      121 gcatgatcgg ctgcggcaag ccactgatcg cgcgcagatt gctccagatc ggtcagcgac
      181 tctccgttcc attggcgagt gagcagcagc gttatgtttt caggcaaact catattttgg
      241 gtaattcatc tccaaagaca ccaatcaggg ccgatgcccc tacgcagatt ttgacaaata
      301 cagaaataag cagcccgatg taaggcccca aggcgtttct gagaaaccga agcgctttgg
      361 ttatctggtt ttccactgtt ttgggcgaga tgtccaactg ctcggcaatt tccgcatgcg
      421 acagcgactc aaaccggctc aacacaaaaa ccaagcggca cttttcaggc agttgatcta
      481 tgtattgatc caccattttt tgcaactcct tggcttccaa ttgttccggt atgcccggag
      541 cgccgtccga catagcaggc aattccgcat catcgctcca cttcattttc tggtcgcgca
      601 catagttgag cgcccggttc aaagcggccc tgcgcaaata ggcgcccaat gaagtgctga
      661 ttgtcaattg attgcgcctt ttccaaagct ccaaaaaaac ctcctgtgca atatcctcgg
      721 ccgtttccgc ctgtggcaca accctgtaca ccgtacggca tacctgttcg taatgccgtc
      781 ggaatatcaa cgccatggcc gcttcgccgt ctgtggccag cctctgcatc aattcgttgt
      841 cggtgatttc tgaacgcagc attcgggtgt ttctgaagcg ccaaaaatag aaatagccgg
      901 gcttttggag agaaaaaacg gcaattactt gttgctaaaa acaaaaatag cctaccttaa
      961 cgccccgaaa acctttaaca cgcattcact gcgcctaaac gaaatatttc tcttatgagc
     1021 aaacacaacg aatcctgggg cagccgggta ggtctggtac tggctatggc gggcaacgcc
     1081 gtgggcttgg gcaatttcct ccgcttccct gtgcaggcag tccaaaacgg gggcggcgcc
     1141 ttcatcattc cttatttggt atgtttcctt ttgatgggca ttccgctttt gtatgtagaa
     1201 tggagcatgg ggcgctttgg gggcggcaga ggccaccaca gcacgccgtt tatcctcgat
     1261 tctatggcgc ccaaacagtt cctttggaag tacatcggcg tattcggcat ctttaccaat
     1321 gtagctgtag ccgcatacta ctgctatttg gaaagctgga cgctgggcta catctggcac
     1381 tctatatccg gcactttcaa gggtatggac agcgcgcagg tagccgactt tttcagcggt
     1441 tatactgctt tcgacgatta ctctaatgtc gctttctggg tgttctgtct ggcgctcaat
     1501 acctatattc tctcgcgcgg cctcagcggc ggcgtagaaa aagtggccaa agtgggcatg
     1561 cctttgctca ttgcctttgg ggtgctgctg gccattcggg gcattacgct caagtcgggc
     1621 aacgccggcg ctgtttacga cggcaccgaa ggcctgaact acctgtggac gcccgatttc
     1681 tcctccatct ggacgcccaa agtgtggctg gctgccgccg gacaaatttt ctttaccctc
     1741 tcggtgggca tgggcagtat tcagtgctac gcctcgtatc tgcgcaaacg cgacgacgtg
     1801 gccctcaatg ccatgagcgc cggctggatg aacgaatttg tagaggtagt gctgggcgcc
     1861 gctatagtta ttcccatcac ggtgggctac ttcggcattg acggcatgaa agacttagtg
     1921 cagcaggccg gcggtttggg gcttggtttc cgcacgctgc cttacctgtt ccagcagtgg
     1981 gggccggtgt tggctgttgt ggcgggtgtg cggtggttcg gactgttgtt ctttgccggc
     2041 attaccagtt cgttggccat gggtacgcct gtattgggct tcctgcgcga tgagtttggc
     2101 tggaaacgca cttcggcagc atgggcattc ggggccatcg tgttcattct gggacttcct
     2161 accgtgttgt ttttcaaata cggcgttttc gatgagtacg actactgggc gggcacggtc
     2221 tcgctggtgg tgtttgccat gttcgagatt attctcttcg cctgggtatt cggtatagac
     2281 aaaggctggg aggaaattac acgcggcgca gacatgacgg tgccgatcat tttcaaatac
     2341 atcatcaagt ttgtgacgcc ggccattctt atttttgtat tcgtgggcag cctgccgggc
     2401 atctgggatg cgatcaccaa caaggcgatt tacgaacaaa ttgtaaaagc aaccgatggc
     2461 gcagtcatag aggagttgaa acgcaagatt ttcttcgtca atatctcgcg cattttgctg
     2521 gtggctgttt ggggcttcat cgcgtttttg gtgtataagg cttacgccaa acgccgccgc
     2581 gaaggccgct tcactacatc ggagatttga ttttatttca aaaaacaaag cagcgtcggc
     2641 agttgaatcg gcgctgcttc ttcaccatta atatctcaca cttatgaata ccatcgcgct
     2701 tatcacctgg ctggcttccg tcattacggt cacgggcttt accgggtact ttttctaccg
     2761 ggtactcacc aatccgcacg cacccgatgt gccgcactcc gatgatgtgg ctcaaggtcc
     2821 taaaactttt gatgctacat aaaataggcg tcacttcaac aaccaatgca gggccagcgc
     2881 agcagcggcc gccgatgaag gcgtgccaat gcnnnnnnnn nnnnnnnnnn nngccctgtt
     2941 tttgttggcg agggtcgtaa gctacatgaa acccatattc tgaaaccaga tgcaaagtgc
     3001 tgcgacgcag gcgctctgcc gtttcattaa aatcataccg tttcagccct ttatacaaca
     3061 tccagttcag ggccggccac acggcgccgt tccatccccc gcaatgatct gaagctattt
     3121 ggcttgttct gcacgatggg tacattctgt acgcagggtc ggtccatgcc gggctttcga
     3181 gcaatgccaa catacgctcg gcctgcgcct gatcggggat ttctgccagc atgggcaaga
     3241 ggccggctaa tgtaggtacc ttgataaatc ggcctgtact caaatcatag gcccgataag
     3301 aggccgcttc ctcatcccac aacttctcgt tcatggtatg caccgtcagt tcgtaccagt
     3361 gcgccgcctc gctgatgtct tctttcagaa aatgccccag cgccatcagc gcctcattgg
     3421 aagcggctaa gcaggtatta aaaaaagggt cttgtatttg aagcgtggga gagttccggt
     3481 accaatagcc cggtgcattg ccgaatccat cttcttcagg atgctcaatc acgagcaagc
     3541 cttcttcggc tggatcgcga tgtgcgtaaa gatagcggtg gtatgctatg gccctatgaa
     3601 aagctgtgcg aagggccggc gccgaaaact cagcgctttt tttggaagat tgccggagtt
     3661 gttccaaaat gaaaccctga atcggcaagg cagcagctcc cgtccggttg cctgatggca
     3721 gaaaacctgt cggggtttgt gcagcaaaaa ggtagccgca aagcgtttca gcgtcagagg
     3781 tttccgacag tgggagaccc catattctga aatccgacat ccagagaggc ccgtccaaag
     3841 atgttttgtt gcgagcggcg gccagcgtgc cggcggcctc catcagtgat tttttcaagg
     3901 tgtttcaaag tgttgtaaaa ctcaggaagt cattttgtcc aataaaaagt ttgaaaaaga
     3961 atggtcggtt ataaaattct tcctaatttg cccaccaaaa taaaccaaaa acaaagtatt
     4021 atgagcaaat ttgatgaagc ggtagaaaaa tactccgcag acctgaagga tctcggcgtt
     4081 gctttcaacg ctgaattgtt gtcggccgta accaaaggtt taggcccttc tatctacaat
     4141 caggattcct ctatggtagc ctgttcggat gccgaagaat tggctcgcgt aaagactaat
     4201 ttcctgatcg ggaaattggg tctcagcgac ggtccgaagt tggatgaggc gctgagtgca
     4261 gtgtgcagtc aatacgacaa gcgcttcaag catcgcgctg tgttttatta cttattggta
     4321 gagaaattgg gcaaacagtc tgtttatgcc tgattttagc ctgcaatagg tatctgttga
     4381 aagccgcatc ggaaatttat cctgtgcggc tttcttgttt tagggcttca tcaaaagctc
     4441 cagctctttg cgtacaaact cgcccaggct acgcacattc tcgcgcgaaa aatcaaaacg
     4501 aatgccggcg gcctcgtaaa tctctccgat agggcgggtg tatcccaact tcaatgcgtt
     4561 gataaaattg gagatagttg catcggggtt ttcccggtat tgtttccaga tggcgatagc
     4621 tccgagctga gccatgccgt attcgatgta atagaacggc acctcaaaaa tgtgcaattg
     4681 cttgtgccac aggtactcag agtatccctc cacttcgctg ggggccacct cgggcgaact
     4741 gaattccaac aaagtctcca tccaggcagc tttgcgttcg gcccggttgt gttgaggatt
     4801 cagatagagc cagtgttgga atttgtcaat ggtagcgatc cagggaagga ccttcagcac
     4861 aaattcaagt tgagcaaatt ttgcccgccg aaggtcctcc tgatcaggga agaaaacatc
     4921 ccagtgttcc atagtgagga gttccatagt catggcagcc agttcggcta cttccgaagg
     4981 tactcgtttg gcggttttaa ggggataatg cctcgtgagg aaagaatgta tggcatggcc
     5041 gctctcgtgc atgagcgtgc gcaaatcgtt gaaagaattg gtggcgttca taaaaatgaa
     5101 cggtacaccg gtcgccatca ggggcatgtt atagccgccg gggcgcttgc cagggcgtga
     5161 ctccagatcc aaatggccta tttcattcat tttggcaata tacacgccaa aagac
//
LOCUS       DAORLF010000348         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8714_length_5202_cov_2.097338, whole genome shotgun sequence.
ACCESSION   DAORLF010000348 DAORLF010000000
VERSION     DAORLF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8714_length_5202_cov_2.097338"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PK198_21015"
     CDS             complement(<1..937)
                     /locus_tag="PK198_21015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF41292.1"
                     /translation="MQNIKNLLLAGLCALLTMPMLSAQTPGDTIARLFRATEQSLVLL
                     RNEGALLPLQHLDSLRVAYYDLNDQKGSAMEKTLAKYTVLETPALPEGLSAGDAALWA
                     DQQARLYDVCVVALRDVAGDAALPGYLKHQFLLRALMERRPVVAVTVGTGRAWEFYTQ
                     LGLARASIHCPMPGMWSESLAAQAVFGAVGLNGVLENDLGATFKRGHGLRMDAAGRLR
                     YSPPAAVGMDEQYLRDSVAAIVRQGIAGGAYPGAQVLVARHGHVVYHEAFGFHTYDNV
                     RPVTTEDIYDFASVTKITTGLPVAMKLYGEGSLDLD"
     gene            complement(951..>1773)
                     /locus_tag="PK198_21020"
     CDS             complement(951..>1773)
                     /locus_tag="PK198_21020"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRF41293.1"
                     /translation="RKTRLDGKLHYLPNPDFVNDRFRMYVWNNVLGWGAEDLAYRMAN
                     AGYEVVLGPVSNLYFDMAYHNDFDEPGYYWGAYTDVDKAFSFVPFDYFKTAAQDRFGN
                     PIDPAIFVGKSRLTDYGKGKIVGIQGLIWSETLHGPHDLEFMVFPKLLGLAERAWSPA
                     PAYETTADSAQWLSQYAEAWAHFANQLGQRELPRLDFYTGGIYYRIPTPGAVVRNGML
                     HANIQLPGFAIRYTTDRSDPTINSPLYAAPMPVSGRVKLRAFDTRGRGSRVVMVE"
     assembly_gap    1774..1882
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1883..3537)
                     /locus_tag="PK198_21025"
     CDS             complement(<1883..3537)
                     /locus_tag="PK198_21025"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF41294.1"
                     /translation="MTRILFIIIFLAITLSLQAQKKAPFKAADLALRWELVQNQYDGK
                     SQALAKLRIHNQGKKKVPAAGWALYFNSVRSYKDAYTRPAGWKVEGLGGDWFRLTPTA
                     DFAGLAPGDSCELPLVSQHWLLNRADAPNGFYVVFDADPGQGYVTKPVRIVPPANPDL
                     LRRGPEDHAGRVTPAMVFAQNEATPRLPASALRPVFPTPVSLEMQPGHYALDGNTVIC
                     AVPSLEREARLLSEQWSTWFGKTAAVLILPAERHHSGISLRLSDKMRDPESYELRIHG
                     SEGVRIEAGGAAGAFYGIQSLKTLLPAQAWSRPLPSAAVPCLTVSDAPRFAYRGMHVD
                     VARNFQTAKEILRILDVMALYKLNKLHLHFSDDEGWRIEIAGLPELTQVGARRGHSAD
                     EREMLHPAYGSGPHPASGTGSGYYSRREFVEILKYATERHIEVIPEIELPGHARAAIK
                     SMDARYERLRAEGRLDEAARYLLRDLEDRSKYLSVQSYNDNVVNVALPSTYAFVEKVC
                     DELLAMYREAGAPLGSIHLGGDEVPNGVWEQSPACRQLMQQQG"
     assembly_gap    3643..3736
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3841..>4134
                     /locus_tag="PK198_21030"
     CDS             3841..>4134
                     /locus_tag="PK198_21030"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF41295.1"
                     /translation="MLRILFLLCLSLGAAQFPNAQPTAEQQRQVDAMFADWNRHGSPG
                     GSLAIVSKGEMVMKQGYGWADLEHQIPNMPHTVFYAGSVSKQFVAMCVLLLEEQ"
     assembly_gap    4135..4144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4145..>5202
                     /locus_tag="PK198_21035"
     CDS             <4145..>5202
                     /locus_tag="PK198_21035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF41296.1"
                     /translation="ISLDDELRKYLPELPDYGTPITIRHAVHHLSGLRDYLTLWQIGG
                     RDYLDRLNKQAVLEMIFRQRALNFAPGTAYSYSNSGYILLAEIVERVSGQSLRQFARQ
                     HIFEPLGMSSSFFNDNTYGLIPNRAFGYRRGAGGGFENLLMRFDLVGSGGLYTTVEDL
                     AKWDRNFYENRLGKKSPQLIEKLLTEGRFSNGQPCGYAGALVPGQYRGLRTFSHTGSL
                     GGYRAAFVRFPEQQLSIILLGNAAEFAPEQKAYAIADVLLAGQLQDRKSPDQTAETNP
                     QAKPLPVLSAQELPSAAGRYYSEELDAFFTISLKNNELHLRPGNLSAIPLRDLASDPS
                     FSLEWERGGKKKEITGMVL"
BASE COUNT         1042 a         1466 c         1427 g         1054 t
ORIGIN      
        1 catccaaatc taagctgccc tcgccgtaca gcttcatcgc caccggcaac cccgtcgtta
       61 ttttcgtcac cgatgcaaaa tcgtaaatat cctccgtcgt caccgggcgc acattgtcgt
      121 aggtatgaaa cccaaacgcc tcgtgataca ccacatgccc gtgccgcgcc accaacacct
      181 gcgcgccggg gtacgcgccc cccgcaatgc cctgccgcac aatggccgcc acgctgtcgc
      241 gcagatattg ctcgtccata cccaccgctg ccggcggcga atagcgcaac ctgcccgccg
      301 cgtccatgcg caggccatgc ccgcgtttga aggtagcgcc cagatcgttt tccaaaacgc
      361 cgttcaggcc caccgctccg aaaacagcct gcgccgccag cgattccgac cacattcccg
      421 gcatcgggca gtgaatggac gcccgggcca agcccaattg ggtgtaaaac tcccaggccc
      481 tgcccgttcc caccgtcacc gctaccaccg gcctgcgctc catcagcgcc cgcaacaaaa
      541 actggtgctt caggtagccg ggcaacgcag catcgccggc cacatctcgc agggccacca
      601 cgcacacatc gtacagccgc gcctgctgat cggcccacaa tgcggcatcg cctgccgaga
      661 gtccttcggg cagcgcgggc gtttccagca ccgtatattt ggccagcgtt ttctccattg
      721 cgctgccttt ttggtcgttc agatcgtaat acgccacccg cagcgagtcc aggtgttgca
      781 aaggcagcaa cgcgccctca ttacgcagca gcaccaatga ttgttcggtg gcccgaaaga
      841 gcctggcaat cgtatcgccg ggcgtttgcg cgctcagcat gggcatcgtc agcagggcgc
      901 acaggcccgc taagaggaga ttttttatgt tttgcataga ttgattttta ctactccacc
      961 atcaccaccc ggctgccccg gccccgcgta tcaaaggccc gcaacttcac ccggccgctc
     1021 accggcatcg gggcggcata cagcgggctg ttgatggtcg ggtcggagcg gtcggtcgta
     1081 tagcgaatgg cgaaccccgg cagttgaata ttggcgtgca acatcccgtt gcgaaccacc
     1141 gcgcccggcg tgggaatgcg gtaataaatg ccccctgtat agaaatcgag gcgcggcaac
     1201 tcgcgctgcc ccagttgatt ggcaaaatgc gcccatgcct ccgcgtattg gctgagccat
     1261 tgcgccgaat cggctgtggt ttcatacgcc ggcgcaggac tccaggcgcg ctccgccagg
     1321 cccagtaatt tggggaacac cataaactcc agatcgtgcg ggccatgcag cgtttccgac
     1381 caaatcaaac cctgtatgcc cacaatcttg cccttgccgt agtcggtcag gcggcttttg
     1441 cccacaaaaa tggccgggtc tatcggattg ccgaaacgat cttgggcggc agttttgaaa
     1501 taatcaaatg gcacaaaaga aaaggcttta tccacatcgg tgtaggcgcc ccagtagtag
     1561 ccgggttcgt caaaatcgtt gtggtaggcc atgtcgaagt agagattgct caccggcccc
     1621 agcaccacct catagcccgc attggccatg cgataggcca agtcttcggc gccccagccc
     1681 aacacattgt tccacacata catgcggaag cggtcgttta caaaatccgg attgggcagg
     1741 tagtgcaatt tgccgtccaa gcgggttttg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnatgccttg ttgctgcatg agttggcgac aggccggaga
     1921 ctgctcccac acgccgttgg gtacctcgtc gccgcccaga tggatggagc caagcggcgc
     1981 gccggcctcg cggtacatgg ccaaaagttc gtcgcatact ttttccacaa aggcataagt
     2041 ggagggcagc gccacattca ccacgttgtc gttgtacgat tgcaccgaca ggtatttgga
     2101 gcggtcttca aggtcgcgca gcagatagcg ggcggcttcg tccaagcggc cctcggcgcg
     2161 gaggcgctca tagcgggcgt ccattgattt gatggcggcg cgggcatggc ccggcagttc
     2221 tatttcgggt atgacctcaa tgtggcgctc ggtggcgtat ttcagtattt ctacaaattc
     2281 tctcctcgaa taatagcctg atcctgtgcc gcttgcaggg tgggggccgg aaccgtaggc
     2341 cgggtgcaac atctcgcgct cgtcggcgga gtgtccccgt cgggcgccca cctgcgtgag
     2401 ttcgggcaaa ccggcaattt cgatgcgcca gccctcgtcg tcgctgaagt ggaggtgcag
     2461 tttgttgagt ttgtacagcg ccatcacgtc caatatgcgc agtatttctt tggccgtctg
     2521 gaagttgcgg gccacgtcca catgcatgcc gcgataggca aagcggggcg cgtcgctcac
     2581 ggtcaggcag ggcacggcgg cggagggaag cgggcggctc caggcttgtg cgggcaacag
     2641 tgttttgagc gattgaatgc cgtaaaatgc gcccgcagcg ccgccggctt ctatgcgcac
     2701 gccctcggag ccgtgtatgc gaagttcata actttcgggg tcgcgcatct tgtccgacag
     2761 gcgcagtgag atgccgctgt ggtgccgttc ggcgggcaaa atgagtactg ccgccgtctt
     2821 gccgaaccag gtgctccatt gttccgacaa cagccgggcc tcgcgctcca atgacggtac
     2881 cgcgcagatc actgtgttgc cgtcgagggc gtaatggccg ggctgcatct ccaacgaaac
     2941 gggcgtggga aacaccggcc gcaaagcgga ggcgggtagt cggggcgtag cttcattttg
     3001 tgcaaacacc attgcgggcg ttacgcgtcc ggcgtggtct tcgggacctc ggcgcagcag
     3061 gtcgggattg gcaggcggca ctatgcgcac gggcttcgtg acatagccct gaccggggtc
     3121 ggcatcaaaa acgacgtaaa aaccattggg tgcatcggcc cggttgagca gccagtgttg
     3181 actcaccaag ggcaactcgc aactgtcgcc gggggccaaa cctgcaaaat ccgccgtcgg
     3241 tgtcaggcga aaccagtcac cgcccagtcc ttccactttc cagccggcag ggcgggtgta
     3301 tgcgtctttg tacgagcgca ccgagttgaa gtacaaagcc cagccggcag cgggtacttt
     3361 ctttttgccc tgattgtgaa tgcgcagttt ggccaatgcc tgcgacttgc catcgtactg
     3421 attttgaacg agttcccatc ggagtgctaa atcggcggct ttaaacggcg cttttttttg
     3481 tgcctgtaag gagagtgtaa ttgccaagaa gatgatgata aaaaggatgc gtgtcattgg
     3541 tgttgctaca gtctgttggt tggaaatcag gcaaatttgg ggaaaatcag ggagaaacga
     3601 gccagaggaa tggggcgata aaaaaataaa gtgatcattt tcnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnntttt gccccactac aaggaagata gttggaatgc tttttatctt
     3781 cgtgacaacg ttgccgctcg cctgcagcta catcgttcaa aaattacaaa aaaatcagtc
     3841 atgctcagaa tcctcttcct gctctgcctc tcccttggcg ccgcacaatt cccgaacgcc
     3901 caacccactg ccgaacaaca acgacaggtg gatgccatgt ttgccgactg gaaccgtcac
     3961 ggatcgccgg gcggatcgct ggccattgtt tccaaaggtg aaatggtgat gaaacaaggc
     4021 tacggctggg ccgatctgga gcatcagatt cccaacatgc cgcataccgt gttttacgcc
     4081 ggctcggtgt cgaagcagtt tgtggctatg tgtgtgcttc ttttggaaga acagnnnnnn
     4141 nnnnatttcg ttggacgacg agctgcgaaa atacctgccc gaactgcccg attatggtac
     4201 gcccattacc attcgccatg ccgtacacca cctcagcggc ctgcgcgact acctgacact
     4261 gtggcaaata ggcggcagag actacttaga ccgactgaac aaacaggccg ttctggaaat
     4321 gattttccgg caacgagcac tcaatttcgc acccggcaca gcgtattcgt acagcaattc
     4381 gggctacatc ctgctggccg aaatcgtgga gcgtgtatcg gggcagtctt tgcggcagtt
     4441 tgcccggcag cacatcttcg agccgctggg catgagcagt tcttttttca acgacaacac
     4501 ctacggcctc atccccaacc gggctttcgg ctaccggcgc ggcgcgggcg gcgggtttga
     4561 aaacctgttg atgcgcttcg atctggtggg ttccggcggg ctttatacta cggtggaaga
     4621 cctggcaaaa tgggatcgca atttttatga aaaccgcttg ggcaaaaaaa gtccgcagtt
     4681 gattgaaaag ctgctcacag agggccggtt cagcaacggg caaccctgcg gctatgcggg
     4741 cgcgctcgtt ccgggccaat atcggggcct gcgcacgttc agccacaccg gttcattggg
     4801 tggctatcgg gcggcctttg tgcggttccc ggagcagcaa ctgagtatta ttttattggg
     4861 caatgcagct gaatttgcac ccgaacaaaa agcctacgcc atcgctgatg tgctgttggc
     4921 cgggcaattg caggatcgca agtcgcccga tcagacggct gaaacgaatc cccaagccaa
     4981 gcccttgccg gttttgtcgg cacaggaatt gccctctgct gcaggccgct attacagcga
     5041 ggaattggat gcttttttta ccatctcatt gaaaaataac gagttgcatc tccgtccggg
     5101 caacctttcg gccataccgc tgagggattt ggcttccgat ccgtcttttt ctttggaatg
     5161 ggaacgcggc ggcaaaaaga aggaaattac cggaatggtg ct
//
LOCUS       DAORLF010000349         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8742_length_5193_cov_2.245815, whole genome shotgun sequence.
ACCESSION   DAORLF010000349 DAORLF010000000
VERSION     DAORLF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_5193_cov_2.245815"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2045
                     /locus_tag="PK198_21040"
     CDS             <1..>2045
                     /locus_tag="PK198_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF41297.1"
                     /translation="FSLNEGQQLRLPDINYKGSAVEMKEVTVTAQRPILEMKPDKMVF
                     NVQNTINGAGSNAMELLRKAPGVVVDNNDNITMLGRAGVQIYIDGKPSPLRGNDLAEF
                     LKSLQADEIDAIELISNPSARFDASGNAGVINIKLKKDKRLGGNGSVNLGHSVGVLPN
                     YQGGISGNYRNKTMNTFGSYNFNAGNWFNTMNMYREQLGLQFDQQSVSGGTSQNHNFK
                     AGADFFLDKKNTIGFLVNGFNSNFSQTGNSRALIGKTGQTELDSILVASTRNIGFRNN
                     LNFNVNYRFDNGKETIWNFDADYGTFRNDGSEDQPNRYWDGTEQVLLSERIYANNTPT
                     DINITTFKLDHERPFLKGKLSAGLKSAIVATDNTFDFFNVIDGQKLLNTDLSNHFVYT
                     ENVNAAYVSFARQFKKLGISAGVRAEQTNSKGILTAMRPVDNAEVKRSYLDFFPSGGI
                     TYAANQNNSFQLNYSRRINRPSYQDLNPFQGRLDELTYEQGNPFLRPEYANNLQLTHT
                     FKYRYNTTLSYSHTRDQITRIVDTAGVSGAYITWLNLADQYAYSLAFSAPVQVRPWWT
                     SFTNLTGSLLQNRADYGDGKIVDLSAATFNVYSQHTFKLPKSWSVEVSGWYNAPSLWG
                     GNFEMSAMWSIDAGIQKKLFGGKGNLRMAVSDIFKSNRWSGVSRFGVLDSVVSGGWD"
     assembly_gap    2046..2201
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2345..2851
                     /locus_tag="PK198_21045"
     CDS             2345..2851
                     /locus_tag="PK198_21045"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF41298.1"
                     /translation="METSVFPILLIFWAMFAIFSAVFRVKENTVVIIERLGSFKDLRH
                     AGWHFKIPIIDRIAGKISLAPQQVKISSEAVANDNTLFKISALITFKVSEARVQHLFY
                     KVPDPERLIKKQVSEAVSVELRRYSPGEAADRKNEIADAVHRELEKSLFEQGLIVIKT
                     EIARIHPA"
     gene            2954..3919
                     /locus_tag="PK198_21050"
     CDS             2954..3919
                     /locus_tag="PK198_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF41299.1"
                     /translation="MFLSPLLIFAGLFAFFVVMYSFFVVRQQSAVIIERWGRFIGARG
                     AGLHFKIPVFDRVAGRVSLKIQQLDVLVETKTKDNVFVKLKVSVQFRVMEDIIYEAFY
                     KLQNPTEQITAYVFDVVRAEVPKLKLDDVFERKDDIAIAVRRELEEAMNEYGYGIVKA
                     LVTDIDPDHAVKNAMNRINAAEREKLAAEYEGESSRILIVAKARAEAESKRLQGQGIA
                     DQRREIAKGLEDSVAVLNNAGINSQEASALIVITQHYDTLQNLGEHSRSQLILLPNAP
                     SAASDMLGQLVASFVASREMGDEMKNTTLKLKEGQKAQALPEPKK"
     gene            complement(3996..4913)
                     /locus_tag="PK198_21055"
     CDS             complement(3996..4913)
                     /locus_tag="PK198_21055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF41300.1"
                     /translation="MKKLLLFLSVVALLSALGLWWLTDHVLPYAPIRPYRVPLEQQPE
                     FAAGALPSHFGLPFDTFTVRSFDSLTLQGYYIPAPDALATVVLLHGIGGCKEHFYSLS
                     RQLNAIGCNAVLFDHRAQGQSEGMFCTFGEKEKYDVSAVVDLVKTRYNDSIIGIYGTS
                     LGGAIGLKSLTIRPDLRFGIIESTYHNLEAVTNEYFRDIAGFYLSPVVKRSLKRSGEI
                     ADFHPFTANPEDFAPQITCPVFMAHGADDEKIPQAFGRKNFDRLASKTKHFERVPGAG
                     HLNLHQVGGEAYWNKLAGFILNAKKQKGD"
BASE COUNT         1284 a         1397 c         1305 g         1051 t
ORIGIN      
        1 cgttcagcct caacgaagga cagcaactcc gcctgcccga catcaactac aaaggcagcg
       61 ccgtagagat gaaagaagtg accgtgacgg cacagcgccc catcttggaa atgaagcccg
      121 acaaaatggt cttcaacgtg caaaacacca tcaatggggc tggcagcaac gccatggaac
      181 tgctccgcaa agcccccggc gtggtggtgg acaacaacga caacatcacg atgctgggcc
      241 gggccggcgt gcaaatttac atagacggca agccctcacc cttgcgcggc aacgacctgg
      301 cggagtttct caaatcgctg caagccgatg aaattgacgc tattgaattg atttccaacc
      361 cttctgcacg tttcgacgca tcgggcaatg ccggcgttat caacatcaaa ctcaaaaaag
      421 acaagcgcct cggcggcaac ggcagcgtca atctggggca ttctgtcggc gtgttgccca
      481 attatcaggg cggcatcagc ggcaactacc gcaacaaaac catgaatacc ttcggcagct
      541 acaatttcaa tgccggcaac tggttcaata ccatgaacat gtaccgcgaa cagttgggct
      601 tgcagttcga ccagcagtcc gtgagcggtg gtacctccca aaatcataac ttcaaggccg
      661 gcgccgattt ctttttagac aaaaagaata ccatcggttt tttggtcaac ggcttcaaca
      721 gcaacttttc acaaaccggc aacagccgcg ccctcatcgg caaaaccgga cagacggaat
      781 tggacagcat tctggtagcc tccactcgca atatcggatt tcgcaacaac ctgaatttca
      841 acgtaaacta ccgcttcgat aacggcaagg aaaccatctg gaacttcgac gccgactacg
      901 gcaccttccg caacgacggc tctgaagatc agcccaaccg ctattgggac ggcacggaac
      961 aagtgctgct ttcggagcgc atctatgcca acaacacacc caccgacatc aacatcacca
     1021 ccttcaagtt ggaccacgaa cggcccttcc tcaaaggcaa actcagcgcc ggactgaaaa
     1081 gcgccatcgt agccaccgac aataccttcg attttttcaa tgtcattgac gggcaaaaac
     1141 ttctcaatac cgacctgagc aaccacttcg tctacaccga aaatgtgaat gccgcttatg
     1201 tgagttttgc ccggcagttc aaaaaactcg gcatctccgc aggcgtgcgc gccgagcaaa
     1261 ccaactcaaa aggcatcctc accgccatgc gccccgtgga caacgcagag gtaaaacgca
     1321 gctatttaga cttcttcccg tcgggcggca tcacttacgc ggccaaccag aacaacagtt
     1381 tccaactgaa ctacagccgc cgcatcaacc ggccttccta ccaggacctc aatccgtttc
     1441 agggccgctt ggacgaactg acctacgagc agggcaaccc cttcctgcga ccggaatacg
     1501 ccaacaacct gcaactcacg catactttca agtatcgcta caacaccacc ctcagctaca
     1561 gccacacccg cgaccagatt acccgcattg tggacactgc cggtgtgtct ggggcataca
     1621 tcacctggct caatctggcc gaccagtatg cgtacagctt ggcgttcagc gctccggtac
     1681 aagtgcgccc ctggtggacc agcttcacca acctgaccgg ctctttgttg caaaaccgcg
     1741 ccgattacgg tgatggcaag atagtggacc tgagcgccgc caccttcaat gtttactcgc
     1801 agcacacctt caagttgccc aaaagctggt cggtagaggt gtcgggctgg tacaacgcgc
     1861 cctcgctctg gggcggcaac tttgaaatga gcgccatgtg gagcatagac gccggtattc
     1921 agaaaaaact cttcggcggc aagggcaacc tgcgcatggc cgtgagcgat attttcaaat
     1981 cgaaccgatg gagcggcgtg agtcgtttcg gcgtgctgga ttccgtggtg agcgggggct
     2041 gggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttcggggag actcgcagct
     2221 cgcggtttga gcgtagttct tagggatttg tgttatattt gtaccaaaat atggtaatcg
     2281 gcgtttcgct gaccatgagc aggatataca aaaatgtgat gatatagaaa aaaacacgca
     2341 tactatggag acctccgttt ttcccattct tctcattttc tgggctatgt tcgccatttt
     2401 ctctgccgtt ttcagagtaa aggaaaacac ggtggtcatc attgagcggc tggggagttt
     2461 caaggatttg cgtcatgcgg gatggcattt taaaatcccc atcatagacc gcattgccgg
     2521 caagatcagc ctggcgcctc agcaagtcaa aatttcttcg gaggctgtcg ccaatgacaa
     2581 tacgctgttc aaaatcagtg cattgataac cttcaaagta tcggaggccc gcgttcagca
     2641 tttgttttat aaagtgccgg accctgaacg attgattaaa aaacaggtaa gcgaggcggt
     2701 gtccgttgaa ctgcgtcgtt acagtccagg agaggctgcc gacagaaaaa acgagatcgc
     2761 cgatgccgtt catcgcgagc ttgaaaaatc gctgtttgaa cagggcctca tcgtcatcaa
     2821 aacagagata gcgcgcatac atccagcata attgccccct gatcgaaaag aaaagaacaa
     2881 aacatccgca acagttgcta tctttacgac gcttgttaaa atctatccaa ttaaatcact
     2941 aaaaatacct gccatgtttc tctctcctct cctcattttc gcaggcctct tcgccttctt
     3001 cgtagtaatg tacagctttt tcgtggtgcg ccaacagtcg gctgtcatca ttgagcggtg
     3061 ggggcgtttt atcggcgctc gcggcgcggg attgcatttc aaaatccctg tattcgaccg
     3121 cgtagccggc cgggtgagcc tgaaaatcca gcaattggac gtgctggtag agaccaaaac
     3181 caaagacaac gtatttgtca aactcaaagt gtcggtgcaa ttccgcgtca tggaagacat
     3241 catctacgag gcgttttaca aattgcagaa tcccaccgaa cagatcaccg cgtatgtgtt
     3301 cgacgtggtg cgcgcggagg ttcccaagct caaattggac gatgtgtttg aacgcaaaga
     3361 cgacattgcc atcgctgtgc gccgcgagtt ggaagaagcc atgaacgagt acggctacgg
     3421 catcgtcaaa gcactggtaa cggacataga ccccgaccac gccgtgaaaa atgccatgaa
     3481 ccgcatcaac gccgccgaac gcgaaaaact cgccgccgaa tacgagggcg aatcttcccg
     3541 catcctcatc gtggccaaag cccgcgccga agccgaaagc aagcgtttgc agggccaggg
     3601 cattgccgac cagcgccgcg aaattgccaa gggcctcgaa gattccgttg ccgtgctcaa
     3661 caatgcgggc atcaactcgc aggaagcatc ggcgctcatc gtcatcacgc agcactacga
     3721 caccctccag aatctgggcg agcacagccg cagccaactc attctgttgc ccaatgcgcc
     3781 cagcgccgcc agcgatatgc tcggccagtt ggtggcttct ttcgtggctt ccagagaaat
     3841 gggcgatgag atgaaaaaca ctacgctcaa gctgaaggaa gggcaaaaag cgcaggcgct
     3901 gccggaaccg aaaaagtgat tctttagctg agcgctgcga aagcagtgag acgatacgct
     3961 gcaaaatgaa agggtgacta aaagcaagct gctgtttagt cgcccttttg cttttttgca
     4021 ttcaaaataa aacccgccaa tttgttccaa tacgcctctc ctccaacctg atgcagattg
     4081 aggtgcccag ctccgggaac ccgctcgaaa tgcttggttt tcgaggctaa gcggtcaaag
     4141 ttttttcgtc cgaatgcctg cgggattttt tcgtcgtcgg cgccatgagc cataaaaacg
     4201 gggcaggtga tttgtggcgc gaaatcctcc gggttggcgg taaatggatg aaaatcagct
     4261 atttcgcccg atcttttcag cgagcgcttc accaccggag acagataaaa acctgcaata
     4321 tcccggaaat actcattcgt gacggcctcc agattgtggt aagtactctc gatgatgccg
     4381 aagcggaggt cgggtcgaat ggtgaggctt ttcaggccga tggcgccgcc cagcgatgtg
     4441 ccgtaaatgc cgatgataga atcgttgtac ctcgttttca ccagatcaac gacggccgaa
     4501 acgtcgtact tttctttttc tccaaaggtg caaaacatgc cttcgctctg cccctgtgcg
     4561 cgatgatcga agaggacggc gttgcagccg atggcgttaa gttggcgact caaagagtaa
     4621 aaatgctctt tgcagccgcc gatgccgtgc agcaatacga ccgttgcaag ggcatcggga
     4681 gcggggatgt aatatccttg cagcgtgagc gaatcgaaac tgcgcacggt gaaggtgtcg
     4741 aaaggcagtc cgaagtggga aggcagcgcg ccggcggcaa attctggttg ctgttccagt
     4801 ggtacgcgat agggccggat gggcgcgtag ggcaatacat gatcggtgag ccaccagagg
     4861 ccgagggcag agagcagggc gacaacggag aggaatagga gcagcttttt catgccaatg
     4921 ggaacacgca ggatgagggg agggttcgcg ccaggggcag taccacgcca ttagcgtggg
     4981 gatgacccga cccgcctgcc tgtgccggcc cgggttgata ttggccggag gggtggccgg
     5041 tagtggcggg ggcgccccga aaaaaactta cgtttatcca ttcttgctaa ttctgcaaga
     5101 tacattgccc ctgcctatcc ctccattttg atctcaatac ccggctgcct ttcgaagccc
     5161 gacaacgaat acccgatgct gagcaggaag tac
//
LOCUS       DAORLF010000350         5170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8789_length_5170_cov_2.186119, whole genome shotgun sequence.
ACCESSION   DAORLF010000350 DAORLF010000000
VERSION     DAORLF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8789_length_5170_cov_2.186119"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..>1431)
                     /locus_tag="PK198_21060"
     CDS             complement(94..>1431)
                     /locus_tag="PK198_21060"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF41301.1"
                     /translation="QATTATTAPSALEPSAAAPAWADGANIYEVNIRQYTPEGSFNAF
                     ATHLPRLKNMGVDILWLMPIFPVSEERRKGTLGSYYAVSDFRQINPEFGTMEDFRAML
                     DEAHRLGLRVILDWVPNHTGWGHTWIKEHPEYYTQNKAGEIVDPIDPKTGESWGWTDV
                     ADLNYDNADMRKAMIGDMLFWIKEVGVDGFRCDVAGEVPDDFWKDATAQLRQAKSDLF
                     MLAEAEHPPHRNDAHFAMSYGWSFHHLMNEIAKGEKNASDVAVWLTEDRAKFRKGYHM
                     QFITNHDENSWNGTEFERMGEAVKTMAVLAFTFDGMPLIYSGQEAGLSKRLAFFEKDT
                     ILWDNLPAYEPFYRSLLDLKHRNKALWNGAAGGEPVIIPVGDSKKVLAYLREKGDQRV
                     VVILNLSPDPQDIVLKDKRLAGSYSNIFANSSTAITPGMSLKLNAWDFLVLDK"
     assembly_gap    1432..1477
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1520..2029)
                     /locus_tag="PK198_21065"
     CDS             complement(1520..2029)
                     /locus_tag="PK198_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41302.1"
                     /translation="MKYTLFVILAAAACKNEPKTNGNTGNAPKAKTEAWAEAAVTAVS
                     SRETALLTKDFWVFEYYVIPGDPQKSMFNRGLWYKFNMNGTFEYGHWEESLGTGSWKL
                     GEYEGKPLLTVDSGTDNSFDMFWEINFGDYSGFEASFVATKDSPQAGTMCKMINIMTR
                     PTKKQFGYE"
     gene            complement(2125..3663)
                     /locus_tag="PK198_21070"
     CDS             complement(2125..3663)
                     /locus_tag="PK198_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HRF41303.1"
                     /translation="MEVSTRVELRNLRYRDYIGLKEAMVQAYSGLGGMVWEEVPIKKL
                     LKIFPEGQLCVIVDDKVVAAALSIIVDYKKFGDNHTYRDITGNEGFDTHDPNGDVLYG
                     IELFVHPQYRGMRLGRRLYDARKELCENLNLRAIMVGGRIPNYKYHSEELSPREYITG
                     VKRKEIYDPTLTFQLSNDFHVKKILRKYLPGDTDSKEYATLLQWDNIYYQEDEVLINA
                     PKQVVRVGLVQWQMRLFDNFDALVQQVEYFVDSVSNYKSDFILFPEFFNAPLMAEFNH
                     LDEPVAIRELAKFTDPLRRKFMELALAYNVNIITGSMPVVEKGKLLNVSYLCRRDGSW
                     DSYYKIHITPSEVNAWGMVGGNKLRAFDTDSGRIGILICYDVEFPELARIYAEQGVQI
                     LFVPFLTDTQNGYNRVRLCSMARAVENECYVAIAGCVGNLPKVDNMDINFAQSAIFTP
                     SDFQFPTNAITAEATPNAEMTIIADVNLELLLELHEVGSVQNLKDRRKDLYEVVYKKR
                     RKKV"
     assembly_gap    3777..3831
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3832..4960
                     /locus_tag="PK198_21075"
     CDS             <3832..4960
                     /locus_tag="PK198_21075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF41304.1"
                     /translation="EALAHPLRVAAAAGTDSDILRLENLDTDLRPPQAAIEATRIALE
                     RDADNSYLPFTGQWELRQAIADKLHRSANAPYSPAQVVITCGATEAMLDALLAVTDPG
                     DEVILTDPTYAGMIYRVRLAGAVPRLVPFRQQFGEWRLDVDALQQAVSPRTKAVFILN
                     PAMPCGAVLTLAEWQAIAKICIERDLWLIYNAAFERVLFDQRKVIHPAALPGMAGRTI
                     TLGSVSKEYGMIGWRVGWMAAPESIVADLAKVHIYNVVTPVGISQAGALAALQTDESE
                     VLRRCRILEERRDVVCEQLAEYAMIPAQGGWSQLVDVSPLGLTGQQASERLLQKGKVA
                     ATPMTHWGEVNAAQFVRLVFSNEPVERLQTLGRRFRAALGK"
BASE COUNT         1026 a         1397 c         1465 g         1181 t
ORIGIN      
        1 attcccgatt tcggattccc gattcaggat ttcggattcc gcctgacgat aaactgtgaa
       61 gcagatttac ttgcagatga actgcgaagc agattacttg tctagtacga gaaaatccca
      121 ggcattgagt ttgaggctca taccgggcgt aatggccgtg ctgctgttgg caaaaatatt
      181 ggaataactg ccggccaagc gcttgtcttt cagcacgatg tcttgtggat ccggactgag
      241 attgaggatg accaccacgc gctgatcgcc tttttcgcgc aggtaagcca gcactttttt
      301 agagtctccg accggaatga tgaccggttc tccgccagcc gcgccgttcc agagcgcttt
      361 gttgcgatgc ttcagatcga gcagactgcg atagaaaggt tcataagcgg gcaggttgtc
      421 ccaaaggatg gtatcttttt caaaaaacgc caatcgcttg ctcagccctg cttcctgacc
      481 ggagtaaatg agcggcattc catcgaaagt gaaagccagt accgccatcg ttttgacggc
      541 ctcgcccatg cgttcaaatt cggtgccgtt ccaggagttt tcatcgtggt tggtgatgaa
      601 ctgcatgtgg tagcccttgc ggaatttggc gcggtcttca gttaaccaga cagctacatc
      661 ggaggcattt ttttcgcctt tggcaatctc gttcatcagg tggtgaaaac tccagccgta
      721 agacatggca aagtgcgcgt cgttgcgatg cggcgggtgt tcggcttcgg ccagcatgaa
      781 gaggtcggat ttggcctgac ggagttgagc cgtggcgtct ttccaaaaat cgtcgggcac
      841 ctcgccggct acgtcacagc ggaatccgtc aacgcctacc tctttgatcc agaagagcat
      901 atctccgatc atggccttgc gcatatcggc attgtcataa ttgagatcgg ccacatcggt
      961 ccagccccag gactcgccgg ttttgggatc aatcgggtca acgatttcac ctgctttgtt
     1021 ttgggtataa tactccgggt gctctttgat ccaggtatgc ccccagccgg tgtggttggg
     1081 cacccagtcg aggatgacgc gcagaccgag gcggtgggct tcgtcgagca tggcgcggaa
     1141 gtcctccatc gtaccgaatt cgggattgat ctgacggaag tccgatacgg cataataact
     1201 gccgagtgtg cctttgcggc gctcttcgga aaccgggaaa atgggcatca gccagaggat
     1261 gtccacgccc atgtttttga gccggggcag gtgcgtggca aatgcgttga agctgccttc
     1321 gggcgtgtac tgccggatgt tgacctcgta gatgttggcg ccgtcggccc aagcaggcgc
     1381 agcggcagag ggttccaacg cggacggtgc ggtggtagcc gtagtcgcct gnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca aaagagagca gcacaagggc
     1501 gctcaggaaa aagatggatt tattcatagc caaattgctt tttggtaggc cgtgtcatga
     1561 tgttgatcat tttgcacatg gtgcccgcct gcggagagtc tttggtagcg acaaaagaag
     1621 cctcgaaccc gctgtaatcg ccgaaattga tttcccagaa catgtcgaag ctgttgtcgg
     1681 taccggaatc aacggtgagc aggggttttc cttcgtattc tcccagtttc caggagccgg
     1741 tgccgagcga ctcttcccag tgaccgtatt cgaaggtccc gttcatgttg aacttatacc
     1801 agaggccgcg attgaacatg cttttttgcg ggtcgccggg gatgacgtaa tattcaaaca
     1861 cccagaagtc tttggtcagg agagccgttt cgcggctgct gacggcggtc acggcagctt
     1921 cggcccatgc ttcggttttg gcttttggcg cgttgcctgt gttgccgttg gtttttggtt
     1981 cgtttttgca ggctgctgct gcaaggatca caaagagtgt gtatttcatg gttattgtta
     2041 tttcaaattt cgagttccgg gttcaaggtt ccgggttgct tactcctcac atactcaccc
     2101 ctcacccctg aaaactacct accgttacac cttcttacgc cgctttttat acaccacctc
     2161 atagaggtct ttgcggcggt ctttcagatt ttgtacgctg cccacctcgt gtagttccag
     2221 caagagttcc aggttgacgt cggcgatgat ggtcatttcc gcgttgggcg tggcttcggc
     2281 ggtgatggca ttggtaggaa actgaaaatc ggagggtgta aaaatggccg actgtgcgaa
     2341 gttgatgtcc atattgtcca ctttgggcag gttgcccacg cagcccgcaa tggccacata
     2401 gcactcattc tctacggcgc gcgccatgga gcagaggcgc acccggttgt agccgttttg
     2461 ggtatcggtg agaaagggaa cgaagagaat ctgcaccccc tgctcggcat agatgcgcgc
     2521 cagttcgggg aactccacat cgtagcatat caggatgccg atgcgcccgc tgtcggtatc
     2581 gaaagcgcgg agtttgttgc cgcccaccat gccccacgcg ttgacttcgg agggcgtgat
     2641 gtgtattttg tagtagctgt cccaactgcc gtcgcggcga cagagataag aaacgttgag
     2701 cagcttgcct ttttccacca ccggcatact gcccgtgatg atgttgacat tgtaggctaa
     2761 ggccagttcc atgaatttgc gccgcagcgg atcggtgaat ttcgccagtt cgcggatggc
     2821 caccggttcg tccaagtggt tgaactcggc catgagcggc gcgttgaaaa attccgggaa
     2881 caggatgaag tccgatttgt aattgctcac gctgtccacg aagtattcca cttgttgcac
     2941 cagcgcgtcg aagttgtcga acaggcgcat ctgccactgc accaaaccca cgcgcactac
     3001 ctgtttgggg gcgttgatga gtacctcgtc ttcctggtag tagatgttgt cccattgcag
     3061 gagggtggcg tactctttgg aatcggtatc tccgggcaga tatttgcgga gtattttttt
     3121 gacgtggaag tcattggaga gttggaaagt gagggtagga tcgtaaattt ccttccgttt
     3181 cacccccgtg atgtactcgc gcggagagag ttcttcggag tggtatttgt agttgggaat
     3241 ccggccgccc accatgatgg cgcggaggtt gaggttttcg cagagttcct tgcgggcgtc
     3301 gtacaggcgg cggcccagac gcatgcccct gtactgcgga tgcacaaaca gttcgatgcc
     3361 gtagagcacg tcgccattgg ggtcatgagt atcgaacccc tcgttgcccg tgatgtcgcg
     3421 gtaggtgtgg ttgtctccga attttttgta gtccacaatg atagacagcg cggcagccac
     3481 cactttgtca tccacgatga cgcagagctg gccttcgggg aatattttga gcagtttttt
     3541 gataggcacc tcttcccaca ccatccctcc gaggccggag taggcctgca ccatagcttc
     3601 tttgaggccg atataatcgc ggtagcggag attgcgcaac tctactctgg tacttacttc
     3661 catatttttg tcggttccga aattgagccg caaagctacg caatgccggg gatattgaat
     3721 gcagaaaaca cgatgcaact tccgacactg aaagaagaag attcttacga tctttannnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaagccct
     3841 cgctcatccc cttcgcgtag ccgccgccgc cggaaccgat tccgacattc ttcgcttgga
     3901 aaacttagac accgacctcc gccctccgca agcggccatc gaagccaccc gcatcgccct
     3961 cgaacgcgac gccgacaaca gctacctgcc cttcaccggc caatgggaac tccgccaggc
     4021 catagccgac aaattgcacc ggtctgccaa tgcaccctac tcacccgctc aggtcgtcat
     4081 tacctgcggc gccaccgagg ccatgctcga tgccctgctc gcagtcaccg atcccggcga
     4141 cgaggtcatc ctcaccgacc ccacctacgc cggcatgatc taccgcgtgc gattggccgg
     4201 cgctgtgccg cgcttggttc cgttccggca gcagttcggc gagtggcggc tcgatgtgga
     4261 cgccttgcag caagccgttt cgccgcgcac aaaggccgtt tttatcctca atccggccat
     4321 gccctgcggc gctgtgctca cgctggccga gtggcaggcc atcgccaaaa tctgcatcga
     4381 gcgcgacctg tggctcattt ataatgcggc gtttgaacgc gtgctgttcg accagcgaaa
     4441 agtcattcac cccgcagcgc tgccgggtat ggccgggcgc accatcacgc tgggcagcgt
     4501 ttccaaagaa tacggcatga tcggctggcg tgtcggctgg atggccgccc cggaaagcat
     4561 cgtcgccgac ttggctaaag tacacatcta caacgtcgtc acgccagtag gcatttcgca
     4621 ggcgggtgcg ctggcagccc tgcaaaccga tgaaagcgag gtgctgcgcc gctgccgcat
     4681 cctggaagag cgccgcgatg tggtgtgcga acagttggcc gaatacgcca tgattccggc
     4741 acagggcggc tggtcgcaat tggtggacgt gtcaccgctg gggcttaccg gccaacaagc
     4801 atccgaacgg ctcctccaaa aaggaaaagt cgcagccacg cccatgacgc actggggcga
     4861 ggtaaatgcc gcgcagtttg tccgcctcgt tttcagcaac gaacccgtgg agcgcctgca
     4921 aacgctgggc cgccgctttc gcgccgcgtt ggggaagtga aggatttatc agtgggcggt
     4981 gagcggtcgg cggtgctaag cgtgagggaa ctaccagtag gcggtggaca gtagccgcgc
     5041 cgtggcgcgg taagcgcggc ttgcagccag tagccagcgg ccagcagccg ttcccacccg
     5101 cagaggcttg cagcttgagg cttgtagcca gcagccgttc ccacccagaa cattgaaccc
     5161 gccttcgcca
//
LOCUS       DAORLF010000351         5167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8798_length_5167_cov_2.319640, whole genome shotgun sequence.
ACCESSION   DAORLF010000351 DAORLF010000000
VERSION     DAORLF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8798_length_5167_cov_2.319640"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK198_21080"
     CDS             <1..285
                     /locus_tag="PK198_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41305.1"
                     /translation="EVAIPGPQKALKLAHAGTDRLVVLFSQKKIETIDTFAKIVSQQA
                     GDLAVNIRATLGAYAVPAADIIYSDDQISFYAATRAEGFIVPVVLEVNAR"
     gene            299..>996
                     /locus_tag="PK198_21085"
     CDS             299..>996
                     /locus_tag="PK198_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41306.1"
                     /translation="MKPIRILLLLLPALSAMSQHLQQDWERQWPGLKINALSQAANGY
                     LLIAGENTAGGKARLLLLERSNGQTIADITANDAEASDFQVVTGTFDGRFAAAGTLGK
                     MARIAWYNDRGQKVQERSFGGTGGKNEFKKIVTAADGSLLAAGHREGRKGGDVWLLRL
                     SGNEVLWEVQLGSEEFETISGLAADRDGGFVFCGTSGKKAPNGAGDAYIAKTDAQGKL
                     LWKKFYGDKDWDEAL"
     assembly_gap    997..1011
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1012..3223
                     /locus_tag="PK198_21090"
     CDS             <1012..3223
                     /locus_tag="PK198_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF41307.1"
                     /translation="ADGGYLIAGLTKSKGAGETDFWLIKTSRDGARQWDKAFGGKNAD
                     VAYAVANAPDGGFLLAGSSLSHRSGARTPMGLLAKTNRAGELSWEQYLGTDKNDELRC
                     LTALFDGSFAAAGFTGGKAWVMHLIDPAAGSLWAGIRDVPALKMSDVKLQTPDETLRP
                     GLASWLALPIENTSDMDVPELRIEVSGAEGGLNVWSTNYAGTLAKGERRTIAIPVESA
                     AAAAEAEHSLRIEVSAGSKSLKTIEKTIAVRREKPAELGIADYRFAPGNASDEVLLTV
                     QLSNTGDQASGPVEMSFSYPAGIRAAGQSAQVLGALAAHSRREVRFSFIKTAQFQESQ
                     VRIVAVAKESGAEKVRKTLEWGASTGRGVIADGPVILWTDPAPHETGTYKVRSNKPEF
                     EYKATILDEKPVKTTDIKVKHNAVLMEGSKFNEQDLSPPARENAYYTYSYRNKIPLAP
                     GRNTLQIWVNGSLSDEIEVEYQPQRSNLHLLAIGAQHEDLKYTAKDAADFAAAFKNQS
                     GEGRLFQNVFTHQLVTPDKTDLTGIKQAFYDLAYLATDQQISPNDVLLVFISSHGKIS
                     ENRFKVLQTGYNPKYERIAIDYKTDILEVLQGIDCKKLIFIDACHSGGAKEGFGAVSK
                     AINDLAIAQPGITTLSSSSSTEKSYEDPAWGNGAFTKAILEAFANTSVSDREGSFQAD
                     ADNDGIIRLGELYNFLRRRVPDLVKTTLPNAPTSQTPFMPENQLDADLPIYLIR"
     gene            3445..>3800
                     /locus_tag="PK198_21095"
     CDS             3445..>3800
                     /locus_tag="PK198_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41308.1"
                     /translation="MPAAGVQTISLTGSLPAITKPNALIDGMAIESAVIDGNLISALG
                     EGIVISASNVTVRGLSLTRFNSPAPGGSGIRISGDNATVNNNYLYNNRIGIIVRDISP
                     TGMVDPAVAPLIRDNF"
     assembly_gap    3801..3834
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3835..>5167
                     /locus_tag="PK198_21100"
     CDS             <3835..>5167
                     /locus_tag="PK198_21100"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF41309.1"
                     /translation="GAASGNSAQGILFENAPSSAIVRNNVISHNGSNGVRANATGGTL
                     RITENSMFCNAAVGIFRLLSPAAPTITEANTQRIRGTASGGNLIEIFTYNDTGCAAGD
                     PCQGRTLLVTTTANALGFWTLNLSAGAVMPGTLVTATRTAGGNDTYAFAGCSAIADCS
                     GFSVSITPTSTTCNGESNGQLTASAPGGSSTTYAWSTGATTPTITGLGPGTYSVTATD
                     AAGCTATASHTLSNPPLLTVGMSGTNITCNGAANGTATATPAGGTPNYQYTWSNGGNS
                     ATISNLGPGTYFVTVADANLCRAFGSVSITQPAALSISCAELNPTSTVGGSDGRATVQ
                     ISAGTAGYTVAWSGAANGSQNQAAAGTATITGLTAGNYNVTVTDANACQQTCNFSIGS
                     PNCNITLQISGTNPTCNGGANGSINLVVSGATGGLNFDWNVNALDGQQNPTG"
BASE COUNT         1198 a         1584 c         1396 g          940 t
ORIGIN      
        1 gaggtggcca ttcccggtcc gcagaaagcc ttgaagctcg cacacgccgg cacagatcgc
       61 ttggtggtgc ttttttctca gaaaaaaatt gaaacaattg atacttttgc aaaaatagtt
      121 agccaacaag cgggcgattt ggcagtcaat atccgcgcca cattgggcgc ttatgccgta
      181 cccgcagccg acatcatcta ctctgacgac cagatttcct tttatgcagc cacccgcgcc
      241 gaggggttta tagtgcctgt ggtgctggag gtaaacgcga ggtaatacta cattgaccat
      301 gaaacccatc cgtattctgc ttttgctctt gccggcgctg agcgccatga gccaacattt
      361 gcaacaagac tgggaacgcc aatggccggg ccttaaaatc aatgccctga gtcaggctgc
      421 gaacggctac ctgctgatag cgggcgaaaa cactgccggc ggaaaagccc gcctgttgtt
      481 gttggagcgc agcaacggcc aaaccatcgc cgacatcacg gccaacgatg ccgaagcttc
      541 tgattttcag gtagtaaccg gcactttcga tgggcggttt gcagccgccg gcacgctggg
      601 caaaatggcc cgcatagcct ggtacaacga ccggggccaa aaagtgcagg aacgcagctt
      661 cggcggaaca ggcggtaaaa atgaatttaa aaagatagtc actgcagcag acggcagcct
      721 gctggccgcc gggcaccgcg aagggcgcaa gggcggcgac gtgtggctgc tgcgcctgtc
      781 gggcaatgaa gtgctgtggg aagtgcaatt gggcagcgaa gaatttgaaa ccatcagcgg
      841 gctggccgcc gaccgcgacg gaggctttgt cttttgcggt acctccggca aaaaggcccc
      901 caatggcgcc ggcgatgcct acattgccaa aaccgacgca cagggtaagc tgctgtggaa
      961 gaaattttac ggcgacaaag actgggacga agccctnnnn nnnnnnnnnn nagccgacgg
     1021 cggttacctc attgccggcc tcaccaaaag caaaggcgcc ggcgaaaccg atttctggct
     1081 catcaaaact tcccgcgacg gcgcccgcca atgggacaaa gccttcggcg gcaaaaacgc
     1141 tgatgtggcc tacgccgtag ccaatgcacc cgacggcggt ttccttctgg ccggctcctc
     1201 gctctcgcac cgcagcggcg cccgcacccc gatgggcttg ttggcgaaaa ccaatcgcgc
     1261 gggggagctg agttgggaac aatacctcgg caccgacaaa aacgacgagc tgcgctgcct
     1321 cacggcgctg ttcgacggca gtttcgcagc agccggattt accggaggca aggcttgggt
     1381 catgcacctc atagaccccg ctgccggcag cctctgggcg ggcatccgcg atgtgcccgc
     1441 gctgaaaatg tcggatgtga aattgcaaac ccccgacgaa acactgcggc ccggcttggc
     1501 ttcctggctg gccctgccca ttgaaaatac ctccgatatg gacgtacccg aactccgcat
     1561 cgaagtgtcg ggcgcagaag gcggtctcaa cgtgtggagc accaactacg caggcacttt
     1621 ggccaaaggc gagcgccgca ccatcgccat tccggtagag agcgccgccg ctgccgccga
     1681 ggccgagcat agcctgcgca tagaggtcag cgccggttcc aagtcattga aaaccataga
     1741 gaaaaccatc gccgtacggc gcgaaaagcc ggcagagttg ggcatagccg actaccgttt
     1801 tgcccccggt aacgcctccg acgaggtgct gctcacggtg caattgtcca acacgggcga
     1861 ccaggcatcc ggcccggtag aaatgagttt cagctatccg gcgggcattc gggcggcggg
     1921 gcaaagcgcg caggtattgg gcgcattggc cgctcacagt cggcgtgagg tgcgcttcag
     1981 tttcatcaaa acggcgcagt ttcaggaatc gcaggtgcgt attgtggccg tagccaaaga
     2041 gagtggcgcc gaaaaagtgc gcaaaacgct ggaatggggc gcatccaccg ggcgcggcgt
     2101 catagcggac ggccccgtca ttctttggac cgatcccgca ccgcatgaaa ccggcacgta
     2161 caaagtgaga tcgaacaaac cggaatttga atacaaggcc accatcctcg atgagaaacc
     2221 tgtaaaaact accgacatca aagtaaaaca caacgccgtg ctgatggaag gttccaaatt
     2281 caacgagcag gacctctcgc cgccggcgcg ggaaaatgcc tactacactt attcctaccg
     2341 caacaaaatt ccgctggcac ccggccgaaa caccttgcaa atctgggtga acggcagcct
     2401 ctcggatgaa attgaagtgg aatatcagcc gcagcgctcc aatttgcacc tcttggccat
     2461 aggggcgcag cacgaagacc tcaaatacac cgccaaagat gccgccgatt ttgccgccgc
     2521 ttttaaaaac cagagcggag aagggcgctt gtttcaaaac gtctttacgc accagctcgt
     2581 cacgcccgac aaaaccgatc tcaccggcat caagcaggca ttttacgatc tggcctacct
     2641 cgctaccgac cagcaaattt cgcccaatga cgtgctgctg gtgttcattt cttcgcatgg
     2701 caaaatttcc gaaaaccgct tcaaagtgct ccaaaccggc tacaacccga aatatgagcg
     2761 catcgccatt gattacaaaa ccgacattct ggaagtattg caaggcattg attgcaagaa
     2821 acttatcttc atagatgcct gtcatagcgg gggcgccaaa gagggcttcg gagcggtaag
     2881 caaagccatt aacgacttgg ccattgcgca gccgggcatt actacgctga gttcgagcag
     2941 cagcaccgag aagtcgtacg aagacccggc ctggggcaac ggcgcgttta ccaaagccat
     3001 tttggaggct ttcgccaata cgagcgtaag cgaccgagag ggcagttttc aggccgatgc
     3061 agacaatgac ggcatcattc gtctgggtga gttgtacaac ttcctgcgcc gccgggtacc
     3121 tgatttggtg aaaaccaccc tgcccaacgc gcccacctcg caaacgccgt ttatgcctga
     3181 aaaccaattg gatgccgacc tgccgatata tctgatccgc tgatgccggg gtcaggcctg
     3241 ccttttttac gaaatacaaa tatcaatgca ccttcgaaac tcatggattc tactgccggc
     3301 cttcctctgt cttgcccaat ggatgcaagc ccaatgcccc acgtccggca atgtgaccgt
     3361 cacgagcaat gccgacgccg gcaccaacac cctccgctgg gccattgact gcatcaacac
     3421 cgtgacacca ccatcgtttt caacatgccc gcagcgggcg tgcaaaccat cagtctcacc
     3481 ggctcattgc ccgccattac caagcccaat gcgctcattg acggtatggc cattgaaagc
     3541 gcggtcattg acggaaatct catttctgca ttgggcgagg gcatagtcat ctccgcttcc
     3601 aatgtgacgg tgcggggcct gagcctcacc cgattcaaca gtcctgcgcc gggaggcagc
     3661 ggcattcgca tctccggaga caatgcaacg gtgaacaata actatttgta taacaatcga
     3721 ataggcatca ttgtccgcga tatttctccc accgggatgg ttgatcctgc agtggcgccg
     3781 ctcatccgag acaatttcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggggg
     3841 cggcttccgg caattcggct cagggcatct tgtttgaaaa cgcgccctcc tcggccatcg
     3901 ttcgcaacaa cgtcatttcc cacaacggca gcaacggcgt gcgcgccaat gccaccggag
     3961 gcactctgcg gataactgaa aacagcatgt tttgcaatgc ggctgtaggc atttttcgcc
     4021 tgctctcgcc ggcagccccc accatcaccg aagccaatac acagcgcatt cgtggcacag
     4081 cgtcgggcgg caatctcatc gaaatattta cttacaacga cacaggctgc gctgccggcg
     4141 acccttgtca gggaagaacg ctgctggtga cgaccaccgc caatgcgctg ggattctgga
     4201 ctttaaacct ttcagccggc gcggtcatgc cgggaacgct cgtcaccgct acccgcactg
     4261 cgggcggcaa cgacacttac gcatttgcag gctgctccgc cattgccgac tgctccggct
     4321 tctctgtttc catcacaccg acgtccacca cctgcaacgg agaaagcaac ggccaactga
     4381 cggcctcagc ccccggcgga agttctacca cctatgcctg gagtaccggc gcgactacgc
     4441 ccacgatcac cggcctcggc cccggaacct actccgtgac ggccaccgat gcggcaggct
     4501 gcacagcaac ggcctcgcac accctgagca atccgccact gctcacggtc gggatgtcgg
     4561 gtacaaatat cacttgcaac ggcgcggcca acggtaccgc caccgccaca cccgccggcg
     4621 gcacgcccaa ctaccaatac acctggagca acggcggcaa ctcggccacc atctccaatc
     4681 tggggccggg cacctacttc gtcactgtgg cagacgccaa cctgtgtcgc gctttcggca
     4741 gtgtgagcat cacgcaaccg gcggccctga gcatttcctg cgcagaactg aatccgacct
     4801 ccaccgtagg tggctccgac ggtcgggcca cagtgcagat ttcggccggc acggcgggct
     4861 acacggtggc ctggagcggg gcggccaacg gttctcaaaa tcaggcggca gccggcacag
     4921 caacgataac cggcttgaca gcaggcaatt acaacgtgac cgtaaccgac gctaacgcct
     4981 gtcagcagac ctgcaatttc agcatcggtt cgcccaattg caacatcact ttgcaaattt
     5041 cgggtaccaa ccccacctgc aacggcggcg ccaacggcag catcaacctc gtggtgagcg
     5101 gcgcaacggg cggcctgaat tttgactgga atgtcaacgc tttggacggg cagcaaaatc
     5161 ccaccgg
//
LOCUS       DAORLF010000352         5144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8856_length_5144_cov_2.034781, whole genome shotgun sequence.
ACCESSION   DAORLF010000352 DAORLF010000000
VERSION     DAORLF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8856_length_5144_cov_2.034781"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK198_21105"
     CDS             complement(<1..200)
                     /locus_tag="PK198_21105"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535533.1"
                     /note="catalyzes the formation of methanethiol and
                     2-ocobutanoate from L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HRF41310.1"
                     /translation="MKKTKDHLRPESRMMSYGYNPALSEGAVKCPIFQTSTFVFRTAE
                     EGKAHFELAYGLRPPKPGEEQGL"
     assembly_gap    285..370
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            496..963
                     /locus_tag="PK198_21110"
     CDS             496..963
                     /locus_tag="PK198_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRF41311.1"
                     /translation="MKTLDALDRRILDFLQQDALLTNKELAARLNMSTTPVFERIRRL
                     EEDGYIRRYVAILDKAKLGFQLTAYCNVQLKEHTKSFLKEFEARVGALPEVIECYHIA
                     GMYDYLLRVVVPDMDAYQNFIVHKLADLPNIGNVQSSFVMTEIKYSTALPVLE"
     gene            complement(967..>2172)
                     /locus_tag="PK198_21115"
     CDS             complement(967..>2172)
                     /locus_tag="PK198_21115"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF41312.1"
                     /translation="SPMEEGIHPQKMVGIDAIANNAVDKKLMPGCQVLIAKNGRIIYS
                     KAFGQHDYKSGREVSTASLYDIASITKVAATTLNIMRLKDEGQLDENDLLKEHLSEAG
                     AANGKITLRQLLTHTSGLQPNLPIGSYLKPVTGKRAACNNYFCKTPRGDYEIEVAEGM
                     YFKKQLRSELIRKLYQLPVAGAKTVRYSDVNMVVLQQVAERTDGRSLNVQAREEFYEP
                     LGLRRIGYRPLKRFSINEIAPTENDTRWRRQVLHGHVHDPAAALLGGVAGHAGLFSNA
                     EDLAVIFQMLLNEGSYGGRQYLKPATVKLFTAKDAKSKRGLGFDKPRKVKYPSFSSEI
                     SEAAFGHTGFTGTCIWADPKEQMIFIFLSNRVHPTASNNAFITSNVRKRIHDVAYNAL
                     GSYQAGWGL"
     assembly_gap    2173..2203
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2204..3835)
                     /locus_tag="PK198_21120"
     CDS             complement(<2204..3835)
                     /locus_tag="PK198_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41313.1"
                     /translation="MVGILEVINEKFRAKLQPVFHLTKLLRWMLTVAAAVALNACNWF
                     ASKDLLRPPYYQFERAQVEAVLEAMSTEQKVAQMLILKTSGADTTGQVQADVAAGLLG
                     GIAAYDLEMKDLAEWLESLDAAAPYPMLHISLETCLLNNQVADATPFPTAYSLAAAAS
                     KDDIENMRKLLRLQTAITGINCISGPGLFPGAVWDAGSGLPKTGADLPLKYIREQSKE
                     GILQIIPSFSHLIYIEKDTAELLPKILAPYRQLAKAGAAGFWIDPRILQPDAARNAVR
                     DYFEEKLQFDGLLAGEGDVEKLALAGADLIVYEGDISEARAILMRLMDERQISRADLD
                     QRVRRILSAKFWTKKLRETLRPQILRDSLSAVQPMLRFSIEDLEYQAARLWRESPVAL
                     HRADAALPLQPGKVRGIRWNDTLPADFWTVLGRYVSLDTVVNLDKEQGTLADAIDTAH
                     INIVLWDSRLSPPEQDTILWQTLNAVADRIVVAHFGDPARLSLADTGVALVHLMERNE
                     NTEAAAAYVLAGAQAARAVLPDSAGVHLPAGAGRRL"
     gene            complement(3814..4659)
                     /locus_tag="PK198_21125"
     CDS             complement(3814..4659)
                     /locus_tag="PK198_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41314.1"
                     /translation="MMKKHEVELIIIGVIMGKEQILYMKIYKDGTLIRSGCGGMPTVP
                     ISGMTVEGSKEYWEQLIAFIDEKIVETPIVYQDENITQPLEYFIAFFGESSNGETGER
                     AKWTKTSGVRFLLDSNTNFRHPLLGFIDSFAIKAAEITNEWYFDIMMSAIYNLQPVHL
                     NGTFVSMPKSEEKEKQEFLSLYVHQIMENGPRGWDIVKIGNGRKYKTTDGTELTASVV
                     NNVGKVSINFFEKVDLGTADQRLMELVSLLEKEGNTTPTESSGLTETTTSEGKHSKNW
                     WEFWK"
     gene            complement(4937..>5144)
                     /locus_tag="PK198_21130"
     CDS             complement(4937..>5144)
                     /locus_tag="PK198_21130"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRF41315.1"
                     /translation="LTQHRAVAPYGMEGGQNGLPGAQWLIAPDGQTERLPGVVQREVP
                     AGWRVRVETPGGGGWGRDEARFLM"
BASE COUNT         1093 a         1392 c         1232 g         1310 t
ORIGIN      
        1 aggccctgtt cctcgccggg tttgggcggg cgcaggccgt aagccagttc aaaatgcgcc
       61 ttgccttcct ccgccgtgcg gaacacgaag gtcgaggtct gaaaaatcgg gcacttcaca
      121 gcgccttccg acagggccgg attgtacccg tagctcatca tgcggctctc cgggcggagg
      181 tgatctttgg tctttttcat gctggtcttt ttttcttttt taggggcgcc ccccgcctct
      241 ggtaaattca gccggcgggg ccgggctatc cactgcatgg cggcnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn cccttttaga tgaatttcct actatttaat taaatcaaag aatataaaac
      421 taccagtaat caatttttaa ttttattttt ccaaaaccta tctttgcgaa ctgcttacac
      481 agaaaaattt tctgcatgaa aacactcgac gctttagacc gccgcatcct cgattttctc
      541 caacaggacg ccctgctgac caacaaggaa ttggccgccc gactcaacat gagtaccacg
      601 ccggtattcg agcgcatccg ccgtttggaa gaagacggct acatccgccg ctacgtcgct
      661 attttggaca aggcgaaact ggggtttcag ctcacggcct actgcaatgt gcaactcaaa
      721 gagcacacca aatcttttct caaagaattt gaagcccgcg tgggcgcgct gcccgaagtg
      781 attgagtgtt accacattgc cggcatgtac gactacctcc tgcgcgtggt ggtgcccgat
      841 atggatgctt accagaattt catcgtacac aaattggccg atctgcccaa tatcggcaat
      901 gtgcaaagct cctttgtgat gacggagatc aaatattcta ccgcgctgcc cgtgctggag
      961 tgagtttcac agcccccagc ccgcctgata cgaccccagc gcattgtatg ccacatcgtg
     1021 gatgcgcttg cgtacattgc tggtgatgaa ggcattgttg gaggcggtgg ggtgtacccg
     1081 attggagagg aaaatgaaaa tcatctgctc tttggggtcg gcccagatgc aggtacccgt
     1141 gaaaccggtg tgcccgaatg cggcttccga tatttcggag gaaaagctgg ggtacttcac
     1201 tttgcggggt ttgtcgaaac ccagaccccg cttcgatttg gcgtctttgg cagtgaagag
     1261 tttcaccgtg gcgggtttca gatattgcct gccgccgtag ctgccctcgt tgagcaacat
     1321 ttgaaaaata acggctaagt cttcggcatt ggaaaacaaa cccgcatggc ctgccacccc
     1381 gcccagcagc gccgccgccg gatcgtgtac atggccgtgc agcacctgcc gccgccaacg
     1441 ggtgtcgttt tcggtgggcg ctatctcgtt gatagagaat cgcttgagtg gccggtaccc
     1501 gatgcgccgc agccccagcg gctcataaaa ctcctcccgc gcctgcacat tgaggctgcg
     1561 cccgtcggta cgctctgcca cctgttgcaa aacgaccatg ttgacatcgc tgtaccgcac
     1621 cgtttttgcc ccggctaccg gcagttggta cagcttgcga atcagttctg agcgcagttg
     1681 ttttttgaaa tacatgccct ctgccacctc tatctcgtaa tcgccgcgag gtgttttgca
     1741 aaagtagttg ttgcaggcgg cccgcttccc ggtcacgggt ttcaggtagg agccgatggg
     1801 caggttgggt tgcagtcctg aagtgtgggt cagcaattgt cgcaaggtga ttttgccgtt
     1861 ggcggcgccg gcctccgaaa ggtgttcctt caaaagatcg ttttcgtcta attggccttc
     1921 gtccttcaat cgcatgatat tcagggtagt agccgccact ttggtaatag atgcaatgtc
     1981 gtacagcgac gctgtgctca cttcgcggcc actcttataa tcgtgttggc caaatgcttt
     2041 ggagtaaata atccggccgt ttttggcaat gagcacctgg caaccgggca tgagtttttt
     2101 atcaacagca ttgttggcaa tggcgtctat acccaccatt ttttgcggat ggatgccctc
     2161 ctccatcggc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaacgt cggcctgctc
     2221 ctgccggcaa atgcacgcct gccgaatcgg gcaataccgc ccttgcagcc tgcgcgccgg
     2281 ccagcacata agctgcggct gcttctgtgt tttcgttgcg ctccatcaga tgcacaaggg
     2341 ccacgccggt atcggccaag ctcagccggg ccggatcgcc gaaatgcgcc actacgatgc
     2401 ggtcggccac cgcattcagc gtttgccaaa ggattgtatc ctgctctggc ggcgataaac
     2461 ggctgtccca cagtacgatg ttgatatgtg ccgtatcaat ggcgtcggca agtgtaccct
     2521 gttctttgtc caaattcacc accgtatcaa ggctgacata gcggcccaaa acggtccaaa
     2581 aatcagccgg cagcgtgtcg ttccaacgga tgccgcgcac tttgccgggc tgcaagggca
     2641 atgccgcatc ggcccggtgc agcgctacgg gcgactcccg ccacaatctc gccgcctgat
     2701 attcaaggtc ttcgatgcta aagcggagca tgggctgcac ggcgctcagg ctgtcgcgca
     2761 gtatttgcgg gcgcagggtt tcgcgcagtt tttttgtcca gaatttggcc gacaggatgc
     2821 gccgcacccg ttggtcgagg tcggcccgac tgatctgccg ctcgtccatc agacgcatca
     2881 aaatggccct cgcctcggat atatctcctt cataaacaat gagatcggcg ccggctaatg
     2941 ctaatttctc cacatcgccc tcgccggcca gcagaccgtc gaattgtagt ttttcctcaa
     3001 aataatcgcg cacggcattg cgggccgcat cgggctgcaa aatgcgcggg tctatccaaa
     3061 agccggcagc gcccgccttg gccaattgcc ggtaaggggc cagtattttg ggcagtagtt
     3121 cggccgtatc tttttctata taaataaggt gagaaaagga gggtatgatt tgcaatatcc
     3181 cttctttcga ctgctcgcgg atgtacttca gcggcaggtc tgcgccggtt ttgggcaggc
     3241 cgctgcccgc atcccaaacc gcgccgggga acagccccgg ccccgaaatg cagttgatgc
     3301 cggtgatggc cgtttgcagg cgcaacagct tgcgcatgtt ctcgatgtcg tccttcgagg
     3361 cggcggcggc taagctgtat gccgtcggga aaggtgtggc atcggccacc tgattattga
     3421 gcagacaggt ctccagcgaa atatgcagca tgggataggg cgcggcagca tcaaggcttt
     3481 ccaaccattc ggctaagtct ttcatttcca ggtcatacgc agcaatgccg cccagcaagc
     3541 ccgcagccac atctgcctgt acctgcccgg tggtatctgc cccggaggtt ttcaggatca
     3601 gcatttgcgc cactttctgc tcggtactca tggcctccaa gacggcttcc acctgtgcgc
     3661 gttcaaactg gtagtagggc ggtcggagca ggtctttaga agcaaaccag ttgcaggcgt
     3721 tcagggccac ggcggcagca accgtcagca tccagcgcag gagcttagtg aggtgaaaaa
     3781 cgggctgtag ctttgccctg aatttctcat ttattacttc cagaattccc accaattttt
     3841 agaatgtttt ccttcagatg tagttgtttc cgttagtcca gaagactctg taggtgtagt
     3901 attgccttcc ttttctaaaa gcgatacaag ttccataagt ctttgatcag cagtacctaa
     3961 atctactttt tcaaaaaaat ttattgatac cttccctaca ttattcacaa cagatgctgt
     4021 taattctgtc ccatcagttg tcttgtattt tctaccattt ccaatcttta cgatatccca
     4081 acctctgggg ccgttttcca ttatttgatg aacatataat gagagaaatt cttgcttttc
     4141 tttttcctct gattttggca ttgacacaaa agtaccgtta agatgtactg gttgcaaatt
     4201 atagatcgca gacatcataa tatcgaaata ccattcattt gttatctcag cggctttgat
     4261 ggcaaaactg tcaataaatc caagaagtgg atgtctgaaa ttagtattgc tgtctaataa
     4321 aaacctgact ccactggttt tcgtccattt tgctctttct ccagtctccc cattactact
     4381 ttctccaaaa aatgcaataa aatattctaa tggttgcgta atgttttcat cttgataaac
     4441 aatcggcgtt tctacaattt tttcatctat gaacgctatt aactgttccc agtattcttt
     4501 gctgccttcc accgtcattc cagagatggg tacagtcggc atacctccgc aaccgcttct
     4561 gatgagtgtg ccatctttgt aaattttcat gtataaaatt tgctcttttc ccattatcac
     4621 tccaatgatg ataagctcga cttcgtgttt tttcatcatt tctgatttag tgtttacaaa
     4681 tattgaatgt ttgggtgctt gtcaaaatga taaacattat ggcaaaggta gcgttttctt
     4741 ggcgccgaac atacttcaat ttacttcact acatggtagt tcgtcaccac gctcactttt
     4801 tcgttttaga tttcactgca gacacacagt cttctctcat cccaaaaaac aaaaaaaaac
     4861 aatcgctggt agcgcggtct cagggcctgc taccactatt tcgttgtcta tggacaactt
     4921 gttgaaaaac aaatatttac atcaaaaacc tcgcctcatc ccgcccccac cctcctccgc
     4981 ccggcgtctc cacgcgcacg cgccagccgg caggaacctc ccgttgcaca acacccggca
     5041 ggcgttccgt ttgcccgtcc ggcgcaatga gccactgcgc gcccggcagc ccattctggc
     5101 cgccctccat gccatacggc gctacggcgc gatgctgcgt cagc
//
LOCUS       DAORLF010000353         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8892_length_5131_cov_1.976950, whole genome shotgun sequence.
ACCESSION   DAORLF010000353 DAORLF010000000
VERSION     DAORLF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8892_length_5131_cov_1.976950"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>537
                     /locus_tag="PK198_21135"
     CDS             <1..>537
                     /locus_tag="PK198_21135"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRF41316.1"
                     /translation="PNYLLQWEAMRWAKAHGCTRYDLWGAPDIFDESDGMWGVYQFKR
                     GFRGTVNRLLITDKYNVQRYDMQDEVFTNAFGTENYLSINGGNDKTQYFASFGYTNND
                     GIIQNTNFTKYTGRLRLNQTLADWATLSAGLSYNYSLSRDLPNGNNFFSPISTMFIID
                     NVWDITERDAAGNLATLTR"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <548..>1226
                     /locus_tag="PK198_21140"
     CDS             <548..>1226
                     /locus_tag="PK198_21140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41317.1"
                     /translation="VEQVRVNPLTVIETFDITQRTNRSIGDVQLNLYPAKGLTVNAIL
                     GYDTYSLVGNEYHPRIPYANVAATFFPDGYVANATSNVTLLNHDLTVNYVKELGETLT
                     STTTAGYQIQNDRNHYTAQEGRDLAPFVRTVSAAANLFTAPVQSIAQRSVWGYFLQQT
                     FGYKEQVFLTLAGRVDGSSAFSPNNQANFYPKVSGSWVLSDYWKDSNLSSILSSVKLR
                     ASYGQAG"
     assembly_gap    1227..1236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1237..2332
                     /locus_tag="PK198_21145"
     CDS             <1237..2332
                     /locus_tag="PK198_21145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41318.1"
                     /translation="PYDRYNNYVTAQLGGLTAVTPSTVLANPDVKPERMTEIEFGADL
                     ALFKNRIGLNVTVYNQDIQDLLLTRPLPPTAGGNFIVTNEGSMTNKGVELLLQTTPVA
                     TSKLRWNLGLMWSSNRNQVDGISGALFLRGSDGAQAAINGQPYGVFYGRYYARDAAGN
                     LLLTSQGLAQPERGVQTTLLEGTPSRAADGQPVASAAQLRKIIGDPNPDWFGAVTSDL
                     TMGKLSFRVQFDAVWGFDVYNWNRITQNNVGQGELAEMEIKGEVARGYVASIAGGVTG
                     ERIQEEHVEDGSFVKLRELALSYDLGKVGKVFNNVNVSLIGRNLISFDKYQGYDPETN
                     SAGQTDRVRGDDFGNVPIPRMMQLRIGARF"
     gene            2371..>2980
                     /locus_tag="PK198_21150"
     CDS             2371..>2980
                     /locus_tag="PK198_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41319.1"
                     /translation="MKIKQYIVLLALPMLLAVGACEREYVNPNAATSADVLTSVDGLL
                     GMVVGLKRSYSVGATGALYNMVSANGLTTKELYVINTGNGELAALEAGGGTVGNSNAF
                     LGNIWASCNIVSANGQLLIDNAGNISDPTTSTAVKVYGHFFKALAIGTMAQFWEQVPT
                     QTSSAEDFLNGKYAAFKPRAQALQDAIALLESGASAIAATPPS"
     assembly_gap    2981..3032
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3033..3694
                     /locus_tag="PK198_21155"
     CDS             <3033..3694
                     /locus_tag="PK198_21155"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF41320.1"
                     /translation="DACNALLARYYNMLGQHDQAIAAADKVNLTRKSIFKFDALNQNP
                     VYRTALVNNNTYDGLNNFGLSGALAPTTGDGRIAFYLGANPPTGVKVQGFFTGDTKEI
                     PVFLPGEILLIKAEALARKNNIAGAITELDKVLTKNNDIFGVNANLPAYSGEQTQTAV
                     LQEIYRNRCIEMYMSGQKLEDSRRFGRPAPPSGERNRNFYPYPLRERDNNPNTPADPT
                     I"
     gene            3807..>4087
                     /locus_tag="PK198_21160"
     CDS             3807..>4087
                     /locus_tag="PK198_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41321.1"
                     /translation="MMTKYLPSLLALLTALSALQCRRTESSGDAAPYFSVTFSGDSAM
                     DGRLLLLISTDDSAEPRFQISDGPGTQQVFGLDVNDWQPGSEAVFTKED"
     assembly_gap    4088..4156
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4157..>5131
                     /locus_tag="PK198_21165"
     CDS             <4157..>5131
                     /locus_tag="PK198_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF41322.1"
                     /translation="VQVLLQKYETFRRADGHVVQLPMDQGEGRQWNRAPGNPYSKPRK
                     VHFDPAKAGNISIELDQIIPPIEPPKDTEYVKHIRIRSKMLSEFWGRDMYLGAHVLLP
                     QGWAEHPDVKYPLAIFHGHFNYDLGGWRTTPPDTTQPCEYNERFQVDCYNRLVQQEAY
                     DFYKMWTGPNFPRVIVIDIQHANPYYDDSYAVNSANLGPYGDAITYELIPYIEQQFRG
                     IDQGWARFLYGGSTGGWEALAAQVFYPAEYNGCYAACPDPIDFRAYTVVNIYEDENAY
                     YTEGPFRKTLRPGTRNALGHISATLRDANYKELALGTRSRSGDQWDIW"
BASE COUNT         1134 a         1497 c         1295 g         1064 t
ORIGIN      
        1 gccgaattat ctgcttcagt gggaggcgat gcgctgggcg aaggcgcatg gctgcacgcg
       61 ctatgacctg tggggtgcgc cggacatttt cgacgaatcc gacggcatgt ggggcgtcta
      121 ccagttcaag cgcggctttc gcggcacggt caaccgcctg ctcatcaccg acaaatacaa
      181 tgtgcagcgc tacgacatgc aggacgaggt gtttaccaat gctttcggta cagagaacta
      241 cctctccatc aacggaggca acgacaagac gcagtacttc gcctccttcg gctacaccaa
      301 caacgacggt atcatccaaa ataccaactt tacgaaatac accggccgcc tgcgcctcaa
      361 ccagacgctg gcagactggg ccacgctttc cgcaggcttg tcctacaatt acagcctcag
      421 ccgcgacttg cccaacggca acaatttctt cagtcccatc agtaccatgt tcatcattga
      481 caacgtgtgg gacatcaccg agcgcgacgc ggccggcaac ctcgcgacgc tgacgcgnnn
      541 nnnnnnnagg tggaacaagt gcgtgtgaat ccgctcactg ttatcgaaac tttcgacatc
      601 acgcagcgta ccaaccgcag catcggcgac gtgcaactca atctgtatcc agctaaaggc
      661 ttgacggtca atgctatttt gggttacgac acctattctt tggtgggaaa tgagtaccac
      721 ccgcgcatcc cgtatgccaa cgtagccgcc actttctttc ccgatggcta tgtggccaat
      781 gctaccagca acgtgaccct gctcaaccac gacctcaccg tcaactatgt gaaagagctg
      841 ggcgaaaccc tgacttcgac caccactgcg ggttatcaga tccagaatga ccgcaaccac
      901 tacactgctc aggaaggccg cgatctggcg ccgtttgtgc gtaccgtttc ggcagcagcc
      961 aacttgttta ccgcacctgt gcagagcatc gctcagcgca gcgtgtgggg ttacttcctg
     1021 caacagacat tcggctacaa agagcaggta ttccttaccc tggctggtcg cgtggacggc
     1081 tcctctgctt tctcgcccaa caaccaagcc aatttctacc cgaaggtcag cggttcctgg
     1141 gtattgtccg attactggaa agacagcaac ctgtcttcta tcctgagttc cgtcaaactg
     1201 cgcgccagct atggccaggc gggcaannnn nnnnnnccct tacgaccgct acaacaacta
     1261 tgtgacggct cagctcggag gtctcactgc cgtgactcct tctaccgttt tggccaaccc
     1321 tgatgtgaag ccggaacgca tgacggaaat cgaattcggc gccgacctcg ctttgttcaa
     1381 aaaccgcatt ggtctgaacg tgacggttta taaccaagac attcaagacc tgctcctcac
     1441 ccgcccgctg ccgcctactg ccggtggtaa tttcatcgta accaatgaag gttcgatgac
     1501 taacaaaggc gtggaattgt tgctgcaaac aacgcccgtg gctaccagca agctgcgctg
     1561 gaaccttggc ctgatgtggt cgagcaaccg caaccaggtg gacggcatca gcggcgcgct
     1621 cttcctgcgt ggcagcgacg gcgctcaggc agccatcaac ggacagccct acggcgtgtt
     1681 ctacggccgc tattatgccc gcgacgcagc cggcaatctt ctgctcacct cgcagggatt
     1741 ggctcagccg gagcgcggcg tacaaacgac tcttctggag ggtacaccca gccgggccgc
     1801 cgacggccag ccggtagctt ccgcagccca gcttcgcaaa atcatcggcg accccaatcc
     1861 cgactggttc ggcgccgtca cgtctgatct caccatgggc aaactctcct tccgcgtgca
     1921 attcgacgcc gtgtggggct ttgatgtgta taactggaac cgcatcactc agaacaacgt
     1981 gggccagggc gaactggcag aaatggaaat caaaggcgaa gtggctcgcg gctatgtggc
     2041 atccatcgca ggcggcgtta cgggcgagcg cattcaggaa gagcatgtag aagacggctc
     2101 tttcgtgaaa ctgcgcgaac tggcgctcag ctacgatttg ggcaaagtag gcaaagtgtt
     2161 taataatgtg aatgtgagcc tcatcggtcg caacctgatc tcttttgaca agtatcaggg
     2221 ctacgacccg gaaaccaact cggccggcca aaccgaccgc gtgcgcggcg acgacttcgg
     2281 caacgtgccc attccgcgta tgatgcaact ccgcattggc gctcgcttct gattttttca
     2341 accaaatttt ggtcatcaaa aaatacaacc atgaaaatca aacaatatat cgttctactc
     2401 gctctgccga tgctcttggc ggtcggcgcc tgcgagcggg aatacgtcaa tcccaatgct
     2461 gctaccagtg ccgatgtgct gaccagtgtg gacggattgc tgggtatggt ggtgggcctc
     2521 aagcgctcgt attcggtggg cgctaccggc gcgctttaca atatggtctc ggccaatggc
     2581 ctgacgacca aagaactgta tgttatcaat accggcaacg gcgagttggc tgcattggaa
     2641 gcaggcggcg gtacggtggg caattccaat gcttttttgg gcaatatctg ggccagttgc
     2701 aacatcgtaa gcgccaacgg tcaattgctt attgacaatg caggcaacat cagcgacccg
     2761 actacttcta ctgcggtgaa agtgtatggc cattttttca aagccttggc tattggtacc
     2821 atggcgcagt tttgggaaca agtacccaca caaaccagct ctgccgagga ttttctcaac
     2881 ggcaagtatg ctgcgttcaa gccgcgcgct caggcgcttc aggatgccat tgcactcctc
     2941 gaatccggcg cttctgccat tgccgctact ccgccatcgg nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgatgcc tgcaatgccc tcctcgcccg
     3061 ctactacaac atgttgggcc agcacgacca ggccatcgct gccgccgata aggtgaacct
     3121 gacgcgcaag tctatattca aattcgacgc gctcaatcag aatccggtat atcgcacggc
     3181 cttggtgaac aacaacacgt atgacggcct gaacaacttc ggtctcagcg gcgctctggc
     3241 cccgacaact ggagacggac gcatcgcctt ctacctcggc gccaatccgc cgacaggcgt
     3301 aaaagtgcag ggcttcttca ccggcgatac gaaggaaatt cccgtgtttt tgcccggcga
     3361 aatcctgctc atcaaagcag aggcgttggc ccgcaaaaac aacatagccg gcgccatcac
     3421 cgagttagat aaggtgctga ccaaaaacaa cgacatcttc ggcgtcaatg ccaacctgcc
     3481 ggcatacagc ggcgaacaaa cgcagactgc cgtattgcag gagatttatc gcaaccgctg
     3541 tatcgagatg tacatgtcag gccagaagct ggaagacagc cgccgctttg gtcgccctgc
     3601 gcctccttcc ggtgagcgca accgcaactt ctatccgtat ccgctgcgcg aacgggacaa
     3661 caatcccaat acgccggccg acccgacgat ttgatcctga gttcattatt agagcatttt
     3721 aatagggcct ctccggtgtt tccggggagg ctttttttta aaattattat ttttgttgac
     3781 ttcttttcat tttcaacaca tccacaatga tgaccaagta cctcccttcc ctgcttgcac
     3841 tgctgacggc cctgagcgct ctgcaatgcc gccgcaccga gtcctccggc gatgctgcgc
     3901 cttatttttc cgtcacgttt tccggcgatt cggcgatgga tgggcggctc ctcctgctca
     3961 tttctaccga cgattcggca gaaccgcgct ttcagatttc cgacgggccg ggcacacagc
     4021 aggtctttgg tttggatgta aatgactggc agccaggctc ggaggctgtt tttactaaag
     4081 aggatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnncgta caggtactct tacaaaagta cgaaaccttc cgtcgcgccg
     4201 acggccatgt cgtacaactg cccatggacc agggcgaagg gcggcagtgg aaccgtgccc
     4261 ccggcaaccc ctacagcaag ccccgcaaag tgcatttcga tcctgcaaaa gccggcaata
     4321 tctcaattga attagatcag atcatcccgc ccatagagcc gccaaaagac accgagtacg
     4381 tcaagcacat ccgcatccgc agcaaaatgc tctccgaatt ctggggccgc gatatgtacc
     4441 tcggcgcgca tgtcctgctg ccgcagggct gggccgaaca ccccgatgtg aaatacccat
     4501 tagccatttt tcacgggcat ttcaactacg acctcggagg ctggcgcacc acgccacccg
     4561 ataccacgca gccctgcgag tacaatgagc gattccaggt ggactgctac aatcgccttg
     4621 tgcagcagga ggcttatgat ttttataaga tgtggaccgg cccgaacttt ccccgcgtca
     4681 tcgtgataga catacagcac gccaatcctt actacgacga ttcctacgcc gtcaattctg
     4741 ccaatctggg gccttacggc gatgccatca cttacgaact gataccctac atcgagcagc
     4801 aattccgagg catcgaccaa ggctgggcgc gatttttata tggcggctcc accggcggtt
     4861 gggaagcgct ggcggcacag gttttttatc ctgccgagta caacggttgc tacgcggcct
     4921 gccccgaccc cattgatttc agggcttata cggtggtcaa tatttacgaa gacgaaaacg
     4981 cctactacac cgaggggccg ttccgaaaga cgttgcggcc gggaacacgc aatgcgctgg
     5041 ggcatatctc agccaccttg cgggatgcaa actacaaaga gttggccctg ggtacgcgca
     5101 gccgttcggg cgatcaatgg gacatttggg a
//
LOCUS       DAORLF010000354         5117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8921_length_5117_cov_1.713354, whole genome shotgun sequence.
ACCESSION   DAORLF010000354 DAORLF010000000
VERSION     DAORLF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_5117_cov_1.713354"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK198_21170"
     CDS             complement(<1..388)
                     /locus_tag="PK198_21170"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF41323.1"
                     /translation="MEPQAIVAILDANVLYPAPLRDFLLSLAFRGVFAPKWSSDIQEE
                     WTRNLIKNRPDLTQEQLNYTVKLMNTAFPDASVTDFHSSTNSFVLPDEGDRHVLAAAV
                     HTKANFIVTFNLKDFPEQPLETLFIKA"
     gene            complement(392..>613)
                     /locus_tag="PK198_21175"
     CDS             complement(392..>613)
                     /locus_tag="PK198_21175"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HRF41324.1"
                     /translation="ELVRAAEAAEILHVSRPHLIKLLEEGQIAHRRVGRHRRVLLGDL
                     LLFQSRQKALRENALNALVQQAQELNLGY"
     assembly_gap    614..777
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<778..966)
                     /locus_tag="PK198_21180"
     CDS             complement(<778..966)
                     /locus_tag="PK198_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41325.1"
                     /translation="MSRQTAYQSFAALNKYKYSLHLLHLLHLLRTLQPKRTMTSSIAA
                     NSKEEQKLAAQSVATLERL"
     assembly_gap    1190..1221
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1222..1482)
                     /locus_tag="PK198_21185"
     CDS             complement(<1222..1482)
                     /locus_tag="PK198_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRF41326.1"
                     /translation="MSIEEAQQRVDEWIRTVGIRYYNELTNTAILMEEVGEVARLMAR
                     IYGEQSFKTKEAEATAQEDLAGEMADVLFVLICLANQTGVNLS"
     gene            complement(1482..1934)
                     /gene="dtd"
                     /locus_tag="PK198_21190"
     CDS             complement(1482..1934)
                     /gene="dtd"
                     /locus_tag="PK198_21190"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRF41327.1"
                     /translation="MRAVIQRVSEASVTIDGEVFSAIGPGLLILLGIEDADNQEDTDW
                     LCRKIAALRIFNDAGGVMNLPVTEVGGELLVVSQFTLHASTKKGNRPSYIRASKPDVA
                     VPLYEAFVKHLHAVSGLPVRTGVFGADMKVRLLNDGPVTIMMDTKNKE"
     gene            complement(2061..>2652)
                     /locus_tag="PK198_21195"
     CDS             complement(2061..>2652)
                     /locus_tag="PK198_21195"
                     /inference="COORDINATES: protein motif:HMM:NF020129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRF41328.1"
                     /translation="DGSWAGWAEINGLQEPDPADFDDKNAYNKAYEEFLRLITKTKDY
                     QALIKERVDPSQWASSHVGNVYSCSIFLALASALERAAGQPEDMTGSMFGFFGYGSGS
                     KSKVFEGVLQPEWRSIAAGIGIQQQLDCRTVIDYKTYENLHRGRTKTSVMAPEAEFAL
                     HHISQQGNKTGARYYHFVPAEVEVAEAVVMEEIAAK"
     assembly_gap    2653..2699
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2700..>3099)
                     /locus_tag="PK198_21200"
     CDS             complement(<2700..>3099)
                     /locus_tag="PK198_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41329.1"
                     /translation="PRLMVIGSTWGVGTRSVHDFFKPKKSVSKLQLVQEVLQLAGLKE
                     MDAEKILAKLPETLEVHGLLDENDEAFSVRKDTPTFDGPYSNWCYQNRIREAFQHFRQ
                     QEEARGGMTESAQILDRWSRLIFHLPYAFHG"
     assembly_gap    3100..3137
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3138..>3508)
                     /locus_tag="PK198_21205"
     CDS             complement(<3138..>3508)
                     /locus_tag="PK198_21205"
                     /inference="COORDINATES: protein motif:HMM:NF013333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41330.1"
                     /translation="ADAHEDAATLAANAVKQLIDQNELDPRRIGRIYLGTESALDGAK
                     PTATYILEMLRRHYRAQYGPDCFLHCDVVDLTFACVGAVDALHNTVDWVRGDQNRIGI
                     VVASDVAKYELASSGEYTQGA"
     assembly_gap    3509..3719
                     /estimated_length=211
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4100..>5117
                     /locus_tag="PK198_21210"
     CDS             4100..>5117
                     /locus_tag="PK198_21210"
                     /inference="COORDINATES: protein motif:HMM:NF028457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HRF41331.1"
                     /translation="MPSARSRPTSSSTPCAVSNNADERRSVADAFAQSAIWGFKVEAE
                     ENGRVLVDFTPFLLRDAHGVVARLKSSNQGTYRVEDSRSAIYMERTKNFPKNSEFEAA
                     VTFTGEGAGAWLRSVAPTPESITVRMHHSLIELPDAGYKPRAYDPRSGYYAIQYQDYA
                     TPIDQPLVKRFIARHRLEKKDPNAKISEAVEPIVYYLDRGAPEPIKSALMEGAAWWNQ
                     AFEAAGYKNAFQVKELPADADPLDVRYNVINWVHRSTRGWSYGSTVSDPRTGEIIKGH
                     VLLGSLRVRQDFLIAQGLIEAYEKGTQPDPRMLQMALARLRQLSAHEVGHTIGLMHNY
                     ISSVN"
BASE COUNT         1030 a         1272 c         1173 g         1150 t
ORIGIN      
        1 ttgctttaat aaacagtgtt tctaaaggct gttcgggaaa gtctttgaga ttgaatgtga
       61 ctatgaaatt tgcttttgtg tgaaccgccg cagccagaac atgccggtct ccttcatccg
      121 gtaatacaaa agagtttgta gaagaatgaa aatcagtaac tgaggcatcg ggaaatgctg
      181 tattcatcaa tttaaccgtg tagttgagtt gttcttgtgt tagatcaggg cgatttttga
      241 tgaggttgcg cgtccattcc tcttgaatat cggatgacca tttaggagca aatactcccc
      301 gaaaagccaa actcaacaaa aaatctctga gtggggcagg atacaacaca ttggcatcca
      361 ggatggcgac tatagcttgg ggttccatta ttcaatagcc taaatttaac tcctgagctt
      421 gttgaacgag tgcatttaac gcattctctc tcaacgcttt ttggcgactt tgaaacaata
      481 ataaatcgcc aagcaatacc cgtctgtgtc tgcccaccct gcgatgagca atctggcctt
      541 cttccaataa ttttatcagg tgcggcctgg aaacgtgcag tatttcagca gcttcggcgg
      601 cgcgaaccag ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag
      781 ccgctctagt gtggcaacag actgagctgc aagtttttgc tcctctttgc tattggctgc
      841 aatgcttgaa gtcatggttc tttttggctg caatgtacgc aataaatgca ataagtgcaa
      901 taagtgcaat gaatatttgt atttattgag cgccgcaaaa gactgataag ccgtttgcct
      961 tgacaaaacc cgcctagttg gttttccaaa agtgaaatat agaactcatt ttcaaaagca
     1021 cattttgaaa tttccccaag cggaaaatct aatttcttat tcaaaaacga gcttgcggct
     1081 tgaagcttgt agctcatggc ttgggggaat atcaaaaaat atcccaaagt ctcctacctc
     1141 aatttcctat tctccgcatg tcgttgcgca tcgcggcggg ttttcttttn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn ngctcaggtt cacgccggtc tgattggcca gacatatcag
     1261 gacaaacagc acatccgcca tttcgccggc gaggtcttcc tgtgccgttg cttcggcttc
     1321 tttggttttg aacgactgtt caccatagat gcgcgccatc agccgggcca cctcgcccac
     1381 ttcttccatc aaaatggccg tgttggtcag ttcgttgtaa tagcgaatgc ccaccgtgcg
     1441 aatccactcg tccacgcgtt gttgtgcttc ctcaatgctc atcactcttt gtttttggta
     1501 tccatcatga tcgtgacggg gccgtcgttg agcagccgca ccttcatatc tgccccgaaa
     1561 acgcccgttc gtaccggcag gccggaaaca gcgtgcaggt gtttgacaaa cgcctcgtaa
     1621 agaggaacgg caacatcggg tttggaggcc cggatataag aaggacggtt gccttttttg
     1681 gtgcttgcgt gcaaggtaaa ctggctcact accagcaact cgccacccac ctcggtcacc
     1741 ggcaggttca tcacgccccc ggcatcgttg aaaatgcgga gcgcggcaat ttttcggcag
     1801 agccaatccg tatcttcctg attgtctgcg tcttctattc ccaataaaat gagcaatccc
     1861 ggcccgatgg ctgaaaatac ctccccgtct atggtgacgg acgcttcgga tactcgctgg
     1921 atgactgcgc gcatgatgtt tttagggcgc aagatagtgc gttgagggga aaggtggcgc
     1981 gtagttttta tgcagagtga aggggtgaat ccgccggata caatttcgta gtcacccctt
     2041 cactatacta tttcaaagca ttacttggct gcaatttctt ccatcacaac cgcctccgca
     2101 acttccacct ccgccggcac aaagtgataa taccgcgcgc cggttttgtt gccttgctgt
     2161 gagatatgat gcagggcaaa ctcagcttcg ggcgccatca cgctggtttt ggtgcgaccc
     2221 cggtgcaggt tttcgtacgt tttgtaatca atgactgtgc ggcagtcgag ttgctgttgt
     2281 atgccgatgc cggctgcaat gctgcgccat tccggctgga gtactccttc aaataccttg
     2341 cttttggagc cgctgccgta gccgaaaaag ccgaacatgc tgccggtcat gtcttcgggc
     2401 tgccctgccg cgcgctccaa tgcagaagcc agcgccaaga aaatggagca ggaatacaca
     2461 ttgcccacat ggctcgaagc ccactgagac ggatctaccc gttctttgat aagagcctga
     2521 taatcttttg ttttggtaat aagccgcaag aattcttcgt aagctttgtt gtatgcattt
     2581 ttgtcgtcaa agtcggcagg gtcgggttcc tgcaagccgt tgatctcagc ccagccggcc
     2641 caggagccgt cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2701 gccgtgaaaa gcatacggca ggtggaagat caggcggctc cagcggtcta agatttgagc
     2761 gctttcagtc atgcctccgc gggcttcttc ctgttggcgg aagtgctgga acgcctcccg
     2821 gatgcgattt tggtagcacc agttggagta tggaccgtca aaagtgggcg tgtctttgcg
     2881 cacgctgaac gcctcgtcgt tttcgtccag aaggccatgt acctctaatg tttcggggag
     2941 tttggccagg attttttctg catccatttc ttttaaacca gccaactgga gcacttcctg
     3001 caccaattgc agtttggaaa cagacttttt aggtttgaaa aaatcatgca cgctgcgggt
     3061 tcccacgccc caagtggagc caatgaccat caggcggggn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnncgg cgccctgcgt gtattcgccc gaagaggcaa gttcgtactt
     3181 ggccacatcg ctcgctacca caatgccgat gcgattttgg tcgccgcgca cccagtccac
     3241 cgtattgtgc agggcatcca ccgcgcccac acaggcgaag gtgaggtcca ccacatcgca
     3301 gtgcaggaag caatcggggc cgtactgtgc gcggtagtgg cggcgcagca tttccaaaat
     3361 ataagtggca gtgggcttgg cgccgtcgag tgcgctttcg gtgccgaggt aaatgcgtcc
     3421 tatcctgcgc gggtccaatt cgttctggtc tatgagttgt ttcacagcat tggccgccag
     3481 ggtggcagca tcttcgtggg catcggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3721 agtcggtatg ttgcggaaga agcttgggaa ttttctaatt tcggcgctca aaatctacat
     3781 accatgcgtt tctttctgct tctttcgctg gctgttgcct tcagcgccaa cctcgccgcg
     3841 cagacctcca tttcttccaa aactgccggt atgaaagcct tcgacggtta ttttcggttt
     3901 tattgggatg aaaaagaggg ccgtatctgg ctggaaatca atcgttggga tgaagagatt
     3961 ttgtacgtca attccttagc tgccggtgtg ggttccaatg acatcggctt ggaccgcaat
     4021 caactgggcg atacccgcat tgtcaagttt cacagggttg gccccaaaat cctgctcgta
     4081 caacccaact acgatttccg tgccgtcagc aagatcgcgc cccacctcct cctcgactcc
     4141 ctgtgccgtc agcaacaacg ccgacgagcg ccgctctgta gccgatgcct ttgcacagtc
     4201 tgccatctgg ggtttcaagg tcgaagcaga agaaaacggc cgcgtgctgg tggacttcac
     4261 cccctttctg ctgcgggatg cgcacggcgt ggttgcccgg ctcaagtcct ccaatcaggg
     4321 cacttaccgc gtcgaagaca gtcgctcggc catttacatg gaacgcacca aaaacttccc
     4381 gaaaaacagc gaatttgaag ctgccgtcac cttcaccggc gaaggagccg gcgcctggct
     4441 ccgctcagta gcgcccacgc ccgaatccat cactgtgcgc atgcaccact cgctcatcga
     4501 actgcccgat gccggctaca aaccccgcgc ttacgacccg cgctctggtt actatgccat
     4561 tcagtatcaa gattatgcaa cgcccataga ccagcctttg gtcaagcgtt tcatcgcccg
     4621 ccaccgttta gaaaagaaag acccgaacgc caaaatcagc gaggcagtag aacccatcgt
     4681 atattatttg gaccgcggcg cgcccgaacc catcaaatcg gccctcatgg aaggcgccgc
     4741 ctggtggaat caggcttttg aggcggcagg gtataaaaat gcctttcagg tgaaagaact
     4801 gcccgccgac gccgaccccc tcgacgtgcg ctacaatgtc atcaactggg tacaccgctc
     4861 cacgcgcggc tggtcttacg gcagcaccgt ttccgacccc cgcaccggcg aaatcatcaa
     4921 aggccatgtt ttgctcggtt ctctgcgggt gcgccaggat tttctcatcg cgcagggcct
     4981 catcgaagcg tatgaaaaag gtacgcagcc cgatccgcgc atgttgcaaa tggcgctggc
     5041 ccgcctccgt cagctctcgg cgcacgaggt aggccacacc atcggcctca tgcacaacta
     5101 catctccagc gtcaacg
//
LOCUS       DAORLF010000355         5108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8939_length_5108_cov_2.340986, whole genome shotgun sequence.
ACCESSION   DAORLF010000355 DAORLF010000000
VERSION     DAORLF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8939_length_5108_cov_2.340986"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      563..679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            954..2276
                     /locus_tag="PK198_21215"
     CDS             954..2276
                     /locus_tag="PK198_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRF41332.1"
                     /translation="MSNLRFETLQLHAGQEEVEATTRSRAVPIYQTTSYTFRDSEHGA
                     NLFALKEFGNIYTRIMNPTNDVFERRIAALEGGVAALAVGSGQAAQFIALNNILQAGD
                     NFIASSNLYGGTYNQFKVAFKRLGIEARFAQGEQAGDYERLIDANTKAIYVETIGNPS
                     FIVPDFERIAAVAKDHDLPLIVDNTFGAGGYLFRPIEHGAAVVVASATKWIGGHGTSI
                     GGVIIDSGNYNWGNGKFPQFTEPSEGYHGLKFWDVFGTDGPFGNIAFIIRARVEGLRD
                     FGPALSPFNSFLLLQGLETLALRVQRHVDNALELARWLSEHPAVESVNYPGLSGSPGH
                     ENARKYLRNGFGGVLSFTLRGDGKAATQLVDNLKLVSHLANVGDAKTLIIQPSATTHQ
                     QLSEAEQRAAGVLPNQLRVSVGIEHIEDIKADFEQAFARVAAPVLVNG"
     gene            2289..3497
                     /gene="metX"
                     /locus_tag="PK198_21220"
     CDS             2289..3497
                     /gene="metX"
                     /locus_tag="PK198_21220"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRF41333.1"
                     /translation="MSCELRVLLAACSSRLEACSFWRYYKKYEKTNNMTARTLHISQP
                     FPTEGGAVLPELHIAYHTWGKPNAEQDNIVWIAHALTGNSDAASWWPGLVGEGRLLDP
                     DKYFIVCANVIGSCYGSTGPTSIDPRTGQAYGLDFPLITIRDMVWAHRLLQRHLGIRR
                     IRLGIGGSMGGQQIIEWAAAEPELFDRIALLATNARHSPWGIAFNESQRMAIMADHSV
                     QEKHPEAGAKGLEAARAVAILSYRHYHAYHRTQLDPTHDKIEDFLAGSYQRYQGEKLR
                     NRFHAHSYLTLSRAMDSHNVGRGRETVENALAQIRKPALVVAIQSDVLFPPEEQYFIG
                     RNLPEAQVETIESLYGHDGFLIEYDKITPLLQDLLERNWTTPAAKKASDHKQETRPSA
                     GSALPGSERI"
     gene            complement(3538..>4259)
                     /locus_tag="PK198_21225"
     CDS             complement(3538..>4259)
                     /locus_tag="PK198_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41334.1"
                     /translation="FHLAVQFFLHFQFNGWFIFAALALALRWMEGQGIVLQSKRLRLF
                     FTLLLVSCVLTYALAVAWSKPLPVVFGINGTGVTLQLAALLVFLGLVWPHRATILAPL
                     SGWSRSLMLIAFWSLAGKILIQTAVVIPFIAKAAYTIRNYVIGFIHLVLLGAMSSLII
                     SLALHHGMLTRNNRITRIGLAVFVTGLLGSEALLFLQGTLLWAAVGFLPNYYEAIFAV
                     SALIPVGAALVGLGEIQARGS"
     assembly_gap    4260..4288
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4289..4777)
                     /locus_tag="PK198_21230"
     CDS             complement(<4289..4777)
                     /locus_tag="PK198_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41335.1"
                     /translation="MSPLIKNWFRIALLNLVLATLLGALLRYAFVEEVTWIKFRNVLH
                     GHSHAAMLGWLYLALYALLAAHFLPERADFRWYNRLFWLTEFSVLGMLVAFPIQGYGA
                     ASIAFSTLHILLSYAWAWRFWRDTRGEPASWSLRFARAAIVFMIVSTLAIWSIGPILA
                     GGF"
BASE COUNT         1231 a         1433 c         1367 g         1048 t
ORIGIN      
        1 aatgaaacgt ttacttatct aatcctttgg cgaaaatgac cactttattt tttattgccc
       61 cttactcgtc ttcttttttt gataaaattt cggagcgaat gtactcttta ttgagcaaaa
      121 caagccctac gagcaagatg gaaacgagga taatgtctga attcatttta gcaatatgtt
      181 tgaggattat taaaacaaca cacatccgac cagcgctacg cgagccgatc aggttctttt
      241 caattcagtt tcagtgttgt tacaggaatg tgggccaaaa atcttttgca aaagtacacc
      301 tcatagctgt aagtcgcaag ggctgtgtat gaattttatg taaaatagcc attttacggc
      361 atggcatatc gaagagagga ggctctcgca cggacgacac ggcgctaaag cattcattat
      421 caataaataa agcaaaaaaa taattatatt tcctatcact aaagtagatt ttaacgtttt
      481 tttaatgccc cttttacttg acttttccgc cgagtcgcct gtatctttgc cgccgtcatc
      541 tgcgaaatcc ttttgcagat ggattttata aagaacgggt tgagagtaca ggctctgtga
      601 aaccctggca accttgccga cagacaaaag gtgccaaatc ctgccggagc gcaatgatgc
      661 gttgccggaa ctataaaatc tggggtttta tgtttattac catctcatcc atcttgcggc
      721 ccgattctga ggccgacctc gttcgccgtc cggtttcgga ggcggatgtt ccttttgccg
      781 cattttgcgg ttttccctgc tgttgttgtt gctgttgatt ttccgtcggg catagcggta
      841 tgacctgatc gggatgactt tggcgtgtcc ggaagcgtgc tttgtcgttc ctgtcggttg
      901 cctgctgtgg ctgcacgggc atatattttt ttcacctgaa aaatcattca atcatgtcca
      961 acttacgatt cgaaacactc caattacacg ccggccagga agaagtggaa gctaccacac
     1021 gctcccgcgc cgtgcccatt taccaaacca cttcttacac gttccgcgat tcggaacatg
     1081 gcgccaattt attcgccctc aaggagttcg gcaatattta tacccgcatt atgaacccga
     1141 ccaacgacgt gttcgagcgc agaatagcgg cgctggaagg cggcgtggcg gcgctggcgg
     1201 taggctccgg acaggcggca cagttcattg cgctgaacaa catcctacag gccggcgaca
     1261 atttcatcgc ttcgtccaac ctctacggcg gcacctacaa tcagttcaaa gtggctttca
     1321 aacgcctcgg catcgaagcg cgctttgctc agggcgagca ggccggcgat tacgagcgcc
     1381 tcattgatgc caataccaag gccatttatg tggaaaccat cggcaatccg agctttatag
     1441 tgcctgattt tgaacgcatt gcagcggtcg ccaaggacca tgacctgccg ctcatcgtgg
     1501 acaatacctt cggcgcgggc ggttatctgt tccggcccat cgagcacggg gctgcggtgg
     1561 tggtggcatc ggcgaccaag tggatcggcg ggcacggcac ttccataggc ggcgtcatca
     1621 tagacagtgg caattataac tggggcaacg gcaagtttcc tcagttcacc gaaccttctg
     1681 aaggctacca cggcctcaaa ttctgggatg tgttcggcac cgacgggcct ttcggcaaca
     1741 tcgctttcat catccgggcg cgggtggagg ggctgcgaga tttcggcccg gcgctgagtc
     1801 cgttcaactc cttcctgctg ctgcaaggct tggaaacatt agcactgcgc gtgcaacgcc
     1861 atgtggacaa cgccttggaa cttgcccgct ggctgtcgga acacccggcg gtggaatccg
     1921 tcaattaccc cggcctgtct ggcagccctg gccacgaaaa cgcccgcaaa tacctgcgca
     1981 acggcttcgg cggcgtgctg tcgtttacct tgcgcggcga cggaaaagcg gccactcaat
     2041 tggtggacaa cctgaaattg gtgagccatt tggccaatgt cggcgatgca aaaacgctca
     2101 tcattcagcc ctctgccacc acgcaccaac aactgagcga ggccgaacaa cgggccgcgg
     2161 gggtattgcc caaccaactg cgcgtgtcgg tgggcatcga gcacatcgaa gacatcaagg
     2221 ccgattttga gcaggctttt gcccgcgtgg cagcgccggt tttggtgaat ggataatagc
     2281 tgcgagctat gagctgcgag cttcgagtac ttctcgcggc ttgcagctcg cggcttgagg
     2341 cttgcagctt ttggagatat tacaaaaagt acgaaaaaac aaacaacatg accgcccgaa
     2401 ccttacatat ttcccaacca ttcccaaccg aaggcggcgc cgtgctgccc gaactccaca
     2461 ttgcctacca cacctggggc aagcccaatg cagagcagga caacattgtg tggatagccc
     2521 acgctctgac cggcaattcc gatgccgctt cctggtggcc gggcttggtc ggcgaaggca
     2581 ggctgctcga tcccgataaa tacttcatcg tgtgcgccaa cgtcatcggc tcctgctacg
     2641 gcagtaccgg ccccacgagc atagacccgc gcaccggcca agcctacggc ctcgactttc
     2701 cgctcatcac catccgcgat atggtatggg cgcatcgcct gttgcagcgc cacctcggca
     2761 tccggcgcat ccggctcggc atcgggggta gcatgggcgg ccagcagatc atagaatggg
     2821 ctgccgccga acccgaattg ttcgaccgga tagccctgtt ggccaccaac gcccggcact
     2881 cgccctgggg cattgccttc aacgaatcgc aacgcatggc catcatggcc gaccacagcg
     2941 tgcaggaaaa acaccccgaa gctggcgcca aaggcctgga agcagcccgc gccgtggcca
     3001 tcctctctta ccggcattac cacgcctacc accgcacgca gttagacccg acacacgaca
     3061 aaatcgagga ctttctggcg ggcagttacc agcggtatca gggcgaaaaa ctgcgcaacc
     3121 gatttcatgc gcactcctat cttacactga gccgggccat ggattcgcac aatgtggggc
     3181 ggggacggga aacggtggaa aatgcgctgg cacagattcg gaagccggca ttggtggtgg
     3241 ccattcagtc ggacgtgctg tttccgcccg aagagcagta tttcatcggc cggaacttgc
     3301 cggaagcgca ggtggagacc atcgaaagtc tgtatggcca cgacggtttt ttgatagaat
     3361 acgacaagat tacgcctttg ctacaagact tgctggagcg aaattggaca acaccggcag
     3421 ccaaaaaagc aagcgatcac aagcaagaaa cgaggccgtc ggcgggatcg gcgctaccgg
     3481 gatcggagcg gatttgaatt tttgggggtg acccttccca aagctacgag cttcgagcta
     3541 cgagccgcga gcctgtatct cgcccaaccc aaccaacgca gcgcctacgg gaatcaaggc
     3601 actgacggca aaaatagcct cgtagtaatt gggaaggaac ccgacggcag cccaaagcag
     3661 cgtgccctgc agaaaaagca gcgcctcgct tcccagaagt ccggttacaa acacggctaa
     3721 tccgatgcgc gtgatgcggt tgtttcgcgt gagcataccg tgatgcagcg ccagactgat
     3781 gatcaggctg ctcatggcgc ccaataaaac gaggtgaatg aagccgatga cgtagttgcg
     3841 aatggtgtag gcagctttgg cgatgaaggg aatgacaacg gcggtctgaa tgagaatttt
     3901 acccgccaaa gaccaaaacg caatgagcat caggctgcgg ctccagccgg acaagggcgc
     3961 cagaatggtt gcccgatgag gccacaccaa gccgaggaag accaacagcg ccgccaattg
     4021 cagcgttacg cccgtgccgt tgatgccgaa aacgacgggc aggggtttgg accaggccac
     4081 cgccagcgca tacgtgagta cgcaagagac aaggagtagg gtaaagaaca gccgcaaacg
     4141 cttgctctgc aacacaatgc cttgcccttc catccaacgc aaagccagcg ccagcgccgc
     4201 aaaaatgaac cagccgttga actgaaaatg caggaaaaac tgcaccgcca gatgaaaaan
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agccgcccgc caggataggc ccgatactcc
     4321 agatggccaa cgtagaaaca atcatgaaca ctatggccgc acgggcaaaa cgcaacgacc
     4381 aactcgccgg ctcgccccgc gtatcgcgcc aaaaccgcca agcccaggca tacgacaaca
     4441 agatgtgcaa ggtggaaaac gcaatggacg ccgccccgta gccttgtatc ggaaaggcga
     4501 ccagcatacc cagcacgctg aattccgtga gccaaaacag ccggttgtac catctgaaat
     4561 cagccctttc gggcaaaaaa tgagcagcca gcagcgcata taaagccaga tacaaccagc
     4621 ccagcatggc cgcatgagaa tggccgtgca gcacgttgcg gaatttgatc caggttactt
     4681 cttccacaaa ggcatagcgc aacagcgcgc ccagcagcgt agcgaggacc aaattcagca
     4741 gcgctattcg aaaccagttt ttgatgagag gactcattgt gtttgaaaat agagtgagtg
     4801 tgctctatag ctgcgagccg cgagctttga gctacgagtg cagagcgcgg attgaacggt
     4861 cggcggtcaa cagtggccgc gcctgtggtg cggggtgcaa agcgcggatt gaacggtagg
     4921 cagtcacgag aggctccgcc taccgcccac cgcctaccga ttatttctcc ccgtcgttgc
     4981 tacgcataaa ctccaatacg ccgcgggcgt ctccctcact caggttttgg ttgggcatgc
     5041 gcacgaggca ctcttccagc agtttctgtg ccgcggggtc tttttccagc atgacgtcca
     5101 cgttgagg
//
LOCUS       DAORLF010000356         5093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_8973_length_5093_cov_1.597261, whole genome shotgun sequence.
ACCESSION   DAORLF010000356 DAORLF010000000
VERSION     DAORLF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8973_length_5093_cov_1.597261"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>294)
                     /locus_tag="PK198_21235"
     CDS             complement(<1..>294)
                     /locus_tag="PK198_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41336.1"
                     /translation="SSTKGSRYDRNADYSSTTTSATPSNYAYDDSGFDFGRNPEVNRA
                     DDEYDYYYTSRIRRFNRPYYGFDFFDPVYVDANYYDPYLTPGATVLIYDNFNSY"
     assembly_gap    295..314
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    676..737
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            753..>1103
                     /locus_tag="PK198_21240"
     CDS             753..>1103
                     /locus_tag="PK198_21240"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRF41337.1"
                     /translation="MRDQLDRMFKETGHVNAYFPLFIPKSFLSKEAEHVEGFAKECAV
                     VTHHRLMNDPNGKGVVVDPSAKLEEELIIRPTSETIIWNTYRDWISSYRDLPLLINQW
                     ANVVRWEMRTRLFLR"
     assembly_gap    1104..1172
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1173..>1511
                     /locus_tag="PK198_21245"
     CDS             <1173..>1511
                     /locus_tag="PK198_21245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRF41338.1"
                     /translation="IDETLQMLDVYAEFAEQYMAMPVIKGVKTANERFAGAEDTYCIE
                     ALMQDGKALQAGTSHFLGQNFAKAFDVKFLNEENVEEYVWATSWGVSTRLIGGLIMTH
                     SDDEGLVLPPR"
     assembly_gap    1512..1546
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1547..2098
                     /locus_tag="PK198_21250"
     CDS             <1547..2098
                     /locus_tag="PK198_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF41339.1"
                     /translation="PVPKPAPELDEAAKKIMAALKAKGITVKYDTDMRKRPGFKFADY
                     EMRGVPVRLAIGMRDLEQQQVEVARRDTKEKTSQPIEGIENYVAQLLEDIQNNLFQRA
                     LDYRNSHITPVDTFEDFQKVLEEKGGFISAHWDGTTETELKIKELTKATIRCIPLDSQ
                     PEEGRCILTGNPSSRRVLFAMSY"
     gene            2104..>2518
                     /locus_tag="PK198_21255"
     CDS             2104..>2518
                     /locus_tag="PK198_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
                     /protein_id="HRF41340.1"
                     /translation="MPKHTKHGAIVKPAIGQYGRNEWAVIGAPCELIEQWVGSVSNRL
                     SPKWQCAFTDADHQTHSTHVAAADAHQELKSVGDSDVYRLRSWLNDYDALFINGNHFT
                     ARRQIVIIDERKKDSLQRKLDRLTDVQLILLQDNIS"
     assembly_gap    2519..2528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2529..>2758
                     /locus_tag="PK198_21260"
     CDS             <2529..>2758
                     /locus_tag="PK198_21260"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRF41341.1"
                     /translation="PWPFLTEHLQGNAVPILQLSDVAAAASWIDDALTRAVPPLRGLV
                     LAGGKSVRMGSDKGLMEYHGKPQREFTADLLAP"
     assembly_gap    2759..2798
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2799..>3117
                     /locus_tag="PK198_21265"
     CDS             <2799..>3117
                     /locus_tag="PK198_21265"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRF41342.1"
                     /translation="QTAEIEPPYQPLPDTFLDLGPYSALLSAFRAYPDSAWLVVACDL
                     PLLDEGTLQFLAQRRDPSRMATAFNSPDNEFPEPLIAIWEPKAYQILLQFLAQGYSCP
                     RKVL"
     assembly_gap    3118..3249
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3346..4212
                     /locus_tag="PK198_21270"
     CDS             3346..4212
                     /locus_tag="PK198_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRF41343.1"
                     /translation="MRQLKITNKITSRESLALDKYLNDIGKIPMLTVDDEANLARRIK
                     LDDQEALEKLTRANLRFVVSVAKQYQNQGLSLGDLINEGNVGLMKAARRFDETKGFKF
                     ISYAVWWIRQSILQAIVEHSRIVRLPLNKVGSYNKVNEAFISFVQEYERDPTNDELAE
                     ILDMTPKEVANMIRGQSRHVSVDAPINNEEGDATMLDMIRGDENMSPDVELMEESLRE
                     EVQQGLSILSPREVEVLSAYYGLNGSKPLNLEEIGELYNLTRERVRQIKERAIRRLRK
                     SYNRNNLKAYLG"
     assembly_gap    4348..4465
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4514..>5093)
                     /locus_tag="PK198_21275"
     CDS             complement(4514..>5093)
                     /locus_tag="PK198_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF41344.1"
                     /translation="GSFKMGSPEDFTNFFNAVIDAKSFRNIAAYIDGAKSDPSCEIIA
                     GGNYDDSKGYFIEPTIILTSNPGYRTMCEEIFGPVLTIYVYEDAQYEATLELLDRTSP
                     YALTGAIFARDRSAVVTAMNKLRNAAGNFYINDKPTGAVVGQQPFGGARASGTNDKAG
                     SVLNLFRWTSARAIKENFVPPTDYRYPFMEEA"
BASE COUNT         1170 a         1254 c         1207 g          976 t
ORIGIN      
        1 gtacgagttg aaattgtcat aaatcagcac ggtagctccc ggagtgaggt aaggatcgta
       61 gtagttggca tccacataaa cgggatcgaa gaaatcgaaa ccataatacg ggcggttgaa
      121 acggcgaatg cgcgaagtgt agtagtaatc gtactcgtca tctgcgcggt tcacttcggg
      181 gttgcggcca aagtcgaaac cactgtcgtc gtaggcgtaa ttgctcggtg tagcagaagt
      241 cgtcgtactg ctgtaatcgg cgtttctgtc ataccggctg cctttggtag aggannnnnn
      301 nnnnnnnnnn nnnnaggcgc atcccggctc ggatcgaaat aaatgtcgtc gtactgtgcg
      361 ttcgcagtcg tcaggacggc ggccatcagc gtgaagctga gcagaatgga aaatttggtc
      421 attttcatag ttgatttttt aaatagtaat taccaaaggc tcatcagtta ttacaaccgc
      481 aaaataatta cttttgcgcg ctcaaaagtg ttaaaatcag tttaaacctt caaaatcggg
      541 ctgttaaagt tgttaacaat tgaatttgaa ccgacttaac agacctcaaa gataagctaa
      601 aatggcgaaa gaaatcacat caaggagcga agattattcg caatggtaca ttgacattgt
      661 caaaaaagcc gatctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnggt ttgtgggaaa atatgcgcga ccaactcgac cgcatgttca
      781 aagaaaccgg ccacgtcaat gcctacttcc cgctgttcat ccccaagagc tttctcagca
      841 aagaagccga acacgtcgaa ggttttgcca aagaatgcgc cgtcgtcacg caccaccgcc
      901 tgatgaacga ccccaacggc aaaggcgtag tagtggaccc ttccgccaaa ttggaagaag
      961 aactcatcat tcgccccacc tcggaaacca tcatctggaa tacctaccgc gactggatca
     1021 gctcttaccg cgacctgccc ctgctcatca accagtgggc caacgtggta cgctgggaga
     1081 tgcgcacccg cttgtttttg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattgacga aacgcttcaa atgctggatg
     1201 tatatgccga atttgctgaa caatatatgg ccatgccggt catcaaaggg gtaaaaacag
     1261 ccaacgaacg cttcgccggc gcagaagaca cctactgcat cgaagcgctc atgcaagacg
     1321 gcaaagcctt gcaggcaggc acttctcact tcctcggtca gaatttcgcc aaggctttcg
     1381 acgtcaaatt cctcaacgaa gaaaacgtag aagagtatgt atgggctacg tcttggggcg
     1441 tttctacccg cctcatcgga ggtctgatca tgacccattc cgacgacgaa ggtctggtat
     1501 tgccgccccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg tgcccaaacc
     1561 cgcgcccgaa ttggacgaag ccgccaaaaa aatcatggcg gccctcaaag ccaaaggcat
     1621 cacggtgaaa tacgataccg acatgcgcaa acgaccgggc ttcaaatttg ccgattacga
     1681 aatgcgcggc gtgcccgttc gcttggccat cggcatgcgc gacttggagc agcagcaggt
     1741 ggaagtggcc cgccgggata caaaggaaaa aacctcacag cccattgagg gcatcgaaaa
     1801 ttatgtagct caactgttgg aagacatcca aaacaacctc ttccaacgag cgctcgacta
     1861 ccgcaactcg cacatcactc cggtggatac tttcgaggat ttccaaaaag tgctggaaga
     1921 aaaaggcggt ttcatctctg cccattggga cggcactacc gaaaccgaac tgaaaatcaa
     1981 ggagttgacc aaagctacca ttcgctgcat tcctctggac tcacagccgg aggagggccg
     2041 ttgcattctg acaggcaatc ccagcagccg gcgggtgttg tttgcgatgt cctattgata
     2101 aaaatgccga aacacaccaa acacggcgcc attgtaaagc ccgccatcgg gcagtatggc
     2161 agaaacgagt gggccgtcat cggcgcgccc tgcgagctta ttgagcaatg ggtcggcagt
     2221 gtttccaaca ggctttcgcc caaatggcag tgcgccttca cggatgccga tcatcagaca
     2281 cattccactc atgttgcagc cgccgatgcc catcaggaac tcaaatcggt tggcgattcc
     2341 gatgtttacc ggctccgctc ttggctcaac gactacgacg cgctttttat caacggcaac
     2401 cacttcacgg cccggcgcca gatcgtcatc attgacgagc gaaaaaagga ctcgctccaa
     2461 cgcaaattgg accgactgac agacgtgcaa ctcattcttt tacaggataa tatttccgnn
     2521 nnnnnnnncc ctggcctttt ttaaccgagc atcttcaggg aaatgccgtg ccgattttgc
     2581 aactgtcgga tgtggctgcc gcagcaagct ggatagatga cgccttgacg cgggccgttc
     2641 caccgctgcg gggcctcgtg ctggccggag ggaaaagcgt aagaatgggt tccgacaagg
     2701 ggctgatgga ataccacggc aagccgcaac gggaattcac cgccgatctg ctggccccnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cagaccgccg agatagaacc
     2821 tccgtaccag ccgctgcccg acactttcct cgatctgggg ccatacagcg ctctgctttc
     2881 ggcgttccgg gcctaccccg attcggcatg gttggtggtg gcctgcgatc tacccctgct
     2941 ggatgaaggc acgcttcagt ttctggcgca acgccgggat ccctcgcgaa tggccaccgc
     3001 gttcaatagc ccggacaatg aatttccgga gccgctcatc gccatctggg aacccaaagc
     3061 ctaccaaatt ctgctgcaat tcctcgcgca gggctattct tgcccacgca aagtactnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnna aactgtatct ttgccgaagg tgttctctga gggaacattg tgcaacccat
     3301 tttcgtatat tcggcaaacc ttttttcaaa aatctactcg gacgtatgcg ccagctcaaa
     3361 attaccaaca aaatcacctc cagagagagc ttagcattgg acaagtatct caacgacatc
     3421 ggcaaaatac ccatgctgac cgttgatgat gaagccaatc tggcccggcg catcaaattg
     3481 gacgatcagg aggcgctcga aaaactcacc cgcgccaatc tccgttttgt cgtttcggtg
     3541 gccaaacagt atcaaaatca agggctttcg ctgggcgacc tcatcaacga gggcaacgtc
     3601 ggcctgatga aagccgcccg ccgttttgac gaaaccaaag gattcaaatt catctcttat
     3661 gcggtatggt ggattcggca gtccatcctt caggccattg ttgagcactc gcgcatcgtg
     3721 cgattgccgc tcaacaaagt aggctcttac aataaagtaa atgaggcgtt tatctctttt
     3781 gtacaggagt atgaacgcga cccgaccaac gacgagctgg ccgaaattct ggacatgacg
     3841 cccaaagagg tggctaatat gattcgcggc cagagccgcc acgtctcggt ggacgcgccc
     3901 atcaacaacg aagaaggcga cgctaccatg ctcgacatga tccggggcga tgagaacatg
     3961 agtcccgatg tggaactcat ggaggagtcg ctgcgcgaag aggtgcaaca aggactttcc
     4021 atcctctcgc cccgagaagt ggaggtgctt tcggcatact acggcctcaa cggcagcaaa
     4081 ccgctgaatc tggaggagat cggcgaattg tacaacctca cccgcgaacg cgtgcgccag
     4141 attaaggagc gcgccatccg tcgcttgcgc aaatcttaca atcgcaacaa cctcaaggcg
     4201 tatttgggat aggttctcgg taggcggtgt gcggtagccg cgccatggcg cggggtgcta
     4261 agcgcgaaga ttcagtaggc ggtaggcggt tctcagcgtg aggaaacgcg aagcctcgaa
     4321 cccggaacct taaaccggta ggcggtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnatgca gcccgaaaca aaacccgttc ctacccgaaa
     4501 cccagaaccc ggcttacgcc tcctccataa acggatagcg ataatcggta ggcggcacaa
     4561 agttttcttt gatggcccgt gccgaagtcc agcggaaaag attgagcacc gaaccggctt
     4621 tgtcgttggt accgctggcc ctggcgccgc cgaaaggttg ctgccccact accgccccgg
     4681 taggtttgtc gttgatataa aaattgcccg ccgcgttgcg cagcttgttc atagccgtga
     4741 cgacggcgct gcggtcgcgg gcaaagatgg ctccggtgag cgcatacggt gaagtgcggt
     4801 cgagcagttc cagcgtagcc tcgtattgcg cgtcctcata tacatatatg gtgagcacag
     4861 ggccgaaaat ctcctcacac atggtgcggt agcccggatt ggaagtcaga ataatggtcg
     4921 gctcgatgaa gtagcctttg gaatcgtcat aattcccgcc ggcaatgatc tcgcaggaag
     4981 gatcggactt ggcgccgtca atgtaagcgg ctatgtttcg gaaggatttg gcgtctatga
     5041 cggcgttgaa gaaattggta aaatcttcgg gcgatcccat tttgaaagaa cct
//
LOCUS       DAORLF010000357         5063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9032_length_5063_cov_2.244209, whole genome shotgun sequence.
ACCESSION   DAORLF010000357 DAORLF010000000
VERSION     DAORLF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_5063_cov_2.244209"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..2021
                     /locus_tag="PK198_21280"
     CDS             234..2021
                     /locus_tag="PK198_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF41345.1"
                     /translation="MEPTNIQDPEYFHKVVDCQYACPAHTPVPEYIRLIAAEKYTEAY
                     MVNWESNVFPGILGRTCDRPCEPACRRGRIEQEEEPVAICRLKRVAADNKGEVKHLMP
                     TAPAQGNGKKIALIGGGPASLTVARDLAPLGYEIHLYDEWNKAGGMMRTQIPAFRLPE
                     SVLDEETGYILSMGIHTHFNHYVSSMREVLDKGYDAVFVGTGAPKGRDLELPGREEGA
                     KNIHIGIEFLANVAFEHRHSIGKKVVILGGGNTAMDCCRTARRLGGEQVTVVVRSPFN
                     TMKASPWEIQDAQREDIPIYNNMPPKEFVVKDGRLTGIVFEKVRVEYDDKGRRNLVPT
                     GEPDVLMEADDVIIAVGQDNAFPWIERDLGIEFGRWDLPVLDKKTFQSTLPKVFFGGD
                     AAFGPENVITAVAHGHQAAMDLFCNGKDLLTDRPAPTTNLVRQKMGIHEWMYDSAVVN
                     DDRYAVPHEEKTLALSNRKIEVELGFDVKTAFREAQRCLNCDIQTVFYTSRCIECDAC
                     VDICPTTCITFTANSEDEDELRSSLNMPALNKSQDLYVSGALPATGRVMIKDEDVCLH
                     CGLCAERCPTAAWDMQKYFYGVTKATKIL"
     gene            2018..3832
                     /locus_tag="PK198_21285"
     CDS             2018..3832
                     /locus_tag="PK198_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRF41346.1"
                     /translation="MSKVKINDFVIRFANVNGTGSASANDMFSKAVFRMGIPVVAKNI
                     FPSNIQGLPTWFDVRVSADGYLGRREEVDIVVAVNPQSFKKDIAAVRSGGYLIYDSTK
                     PLHPEFHREDIHFIGVPMMQLCMDHYNEPRQQQLFKNMVYIGALIELLDIELEVVQGI
                     IRDQFKKHEKLIEPNFQAIDLGRNYVKAHYEYPLPVRVQRMDKNGDSIMIDGNTATGL
                     GALYGGASVVAWYPITPSTSVAKAFETYAKRYRVDPETGEKRYAIVQAEDELAAIGMV
                     IGAAWNGSRAFTATSGPGVSLMSEFLGLAYYAEIPAVLVNVQRGGPSTGMPTRTQQAD
                     LLSSAYASHGDTKHVLLFPANPAECFEFTADALDLADRLQTPILVMTDLDLGMNFHLS
                     PPLKWDDSRKYDRGKVLNAQQLDEIKDFGRYLDVDGDGIPYRTIPGTHPTKGAFTTRG
                     TSRDEYARYTEDAAAYEHSIRRLDKKWKTASTLVPKPEVYRSRGHALGMIWIGTSHHA
                     AVEAIDLLEQQGIAMDGLRVRAFPFNETVEAFLDEHKLVFVIEQNRDKQFRTLLVNEL
                     ETAPKKLVPIQNFDGMPITADSILKQVLERVGEAEVSR"
     gene            3988..4392
                     /locus_tag="PK198_21290"
     CDS             3988..4392
                     /locus_tag="PK198_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41347.1"
                     /translation="MEPVTTSKMVGAVAGYLAQTLKDQRSVQDFFSDFTDAAVQWVRP
                     LFLTDEGQPKEVIEDLQKDPEEKLNTDAVENTLAKALKKDPSAEALLQTMYETIQAKA
                     AAGQTISIVHSKNVVTGNISTTGNVIIGDNNS"
     gene            4399..>5063
                     /locus_tag="PK198_21295"
     CDS             4399..>5063
                     /locus_tag="PK198_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41348.1"
                     /translation="MSEISHSKNIVTGHIDAQGNVVVGDTIINLKEAAQYKAIEAEIA
                     ELNTRFERTRKRIEKAPDDMDFQMELLEIDAKRSQKQQELDSLRREVLKLAEDFQRIP
                     LNTERLRRAKAHFEQGEFSEARAVLDAEVETMGIELDALLHEKGRLNQKTAENQQHLS
                     DKANEYLILARLTATDYELPGRFEKTTAHFEQSLRAERNAENVFAFAHFLQEHNVLMQ
                     AQPL"
BASE COUNT         1279 a         1440 c         1385 g          959 t
ORIGIN      
        1 gaacaaatca ggcgcgtcgg ccatgctgat ttcgcgcaag cacaggcggg tagtgcgcaa
       61 ttcaggaaaa ggagtcatcg gcaaggtcat ggcaattagg aattgatcta aataattctg
      121 caaaggaaaa cttttttgta attttcaatg cctgtcattt gcgtttcttg cgcctattgc
      181 tatgtttaca gccagaaagc acaaatcatt ttcagtcaaa caaactgcgt ttagtggaac
      241 caaccaacat tcaagacccg gagtattttc ataaagtggt ggactgccaa tatgcctgtc
      301 ccgctcacac tccggtaccc gaatacatcc gcctgattgc tgccgaaaaa tacacggaag
      361 cctacatggt caactgggaa tccaacgtgt ttcccggcat tctgggccgc acctgcgacc
      421 gcccttgcga acctgcctgc cgccgaggac gcatcgaaca ggaggaagaa ccggtggcaa
      481 tatgccgtct caaacgcgta gctgccgaca acaaaggcga ggtgaaacac ctcatgccca
      541 ctgcgccggc acagggcaac ggcaaaaaaa tagccctcat cggcggtggt ccggcctcgc
      601 tcaccgtggc cagagacctg gcgccgttgg gctacgaaat ccacttgtac gacgagtgga
      661 acaaggccgg cggcatgatg cgtacgcaga ttcctgcctt ccggctgccc gaatcggtgc
      721 tcgacgaaga aaccggctac atcctctcga tgggcattca tacgcatttc aaccactatg
      781 tgtcgagcat gcgcgaggta ctggacaagg gctacgacgc tgtttttgtg ggaaccggcg
      841 ctcccaaggg ccgcgacctc gaactgccgg gccgcgaaga aggcgccaaa aacatccaca
      901 tcggcattga gtttttggcc aatgtagcct tcgagcacag gcacagcatc ggcaaaaaag
      961 tggtcatcct tggcggcggc aacacggcca tggactgctg ccgcacggcc cgtcgcctcg
     1021 gcggcgaaca ggtgacggtg gtggtgcgca gcccgttcaa taccatgaag gcatcgccct
     1081 gggaaatcca agatgctcag cgcgaggaca ttccgattta caacaacatg ccgcccaagg
     1141 aatttgtggt caaagacggc cgccttacgg gcatcgtttt tgaaaaagtg cgcgtcgaat
     1201 acgacgacaa aggccgacgc aacctcgtgc ccaccggcga acccgatgtg ctgatggagg
     1261 ctgacgacgt catcatcgcc gtgggacagg acaatgcctt cccctggatc gagcgcgacc
     1321 tcggcatcga attcgggcga tgggatttgc cggttttgga caaaaaaacc ttccagtcca
     1381 cgctgcccaa agtgttcttc ggcggcgacg cagccttcgg gcctgagaac gtcatcaccg
     1441 ccgtagcgca cggtcatcag gcggccatgg atctgttttg caacggcaaa gacctgctca
     1501 ccgaccggcc cgctcctacc accaacctcg tgcgccagaa aatgggcatc cacgaatgga
     1561 tgtacgacag cgcggtggtg aacgacgacc gctacgccgt gccgcacgaa gaaaaaacgc
     1621 tggcactgag caaccgcaaa atcgaagtgg aactcggctt cgacgtaaaa accgccttcc
     1681 gggaagcaca gcgctgcctc aactgcgata ttcaaacggt gttttatacc tcccgctgca
     1741 tcgagtgcga cgcctgcgtg gacatctgtc ccaccacctg catcaccttc acggccaata
     1801 gcgaagatga ggacgaactc cgcagcagcc tcaacatgcc ggccctgaac aaatcgcaag
     1861 acctgtatgt gtcgggagcg ctgcctgcca cgggccgcgt gatgatcaaa gacgaggacg
     1921 tgtgcctgca ctgcggtttg tgcgccgagc gctgcccgac agcggcctgg gacatgcaga
     1981 agtactttta tggtgtgacc aaagcgacaa aaatcctatg agcaaagtga agataaatga
     2041 cttcgtaatt cgttttgcga acgtcaatgg cacagggtct gccagtgcca acgatatgtt
     2101 ttccaaagcc gttttccgta tggggattcc ggtggtggcc aaaaacattt tcccttccaa
     2161 cattcagggc ctgcctacct ggtttgatgt gcgcgtgagc gccgacggct acctcggccg
     2221 gcgggaagaa gtggacattg tggtagccgt caatccgcag agtttcaaaa aagacattgc
     2281 tgcggtgcgt tccggcggct acctgattta cgacagcacc aaaccgctgc accccgaatt
     2341 tcaccgggaa gacatccact tcatcggcgt gcccatgatg cagttgtgca tggaccatta
     2401 caacgagccg cgccagcagc agttgtttaa aaacatggtc tatatcggtg cgctgataga
     2461 attgctcgac atcgaattgg aggtggtgca aggcatcatc cgcgatcagt tcaaaaagca
     2521 tgaaaagctc atcgaaccga actttcaggc aattgatctg gggcgcaatt acgtcaaggc
     2581 gcactacgag tacccgctgc ccgttcgcgt gcaacgcatg gacaaaaacg gcgacagcat
     2641 catgatagac ggcaacacgg ctaccggatt gggcgctctg tacggcggcg cttcggtggt
     2701 ggcctggtac cccatcacgc cttctacctc ggtggccaaa gcctttgaaa cctacgccaa
     2761 acgctaccgg gtggaccccg aaaccggcga aaaacgctac gccattgtgc aagcagaaga
     2821 tgaattggcc gccatcggca tggtcatcgg agcggcctgg aacggctcgc gggcattcac
     2881 ggctaccagc ggtccgggcg tgtcgttgat gagcgagttt ctgggattgg cctactatgc
     2941 cgaaatcccc gccgtactcg tcaacgtgca gcgcggcggg ccttccacgg ggatgcccac
     3001 gcgcacccag caggccgacc tcctctcctc ggcctacgct tcgcacggcg ataccaaaca
     3061 cgttttgctc ttccccgcca acccggcaga gtgcttcgag ttcaccgccg atgcgctcga
     3121 cctcgccgac cgcctgcaaa cgcccattct ggtaatgacc gacctcgatc tgggcatgaa
     3181 tttccacctc tcgccgcccc tgaaatggga cgacagccgg aagtacgacc ggggtaaagt
     3241 gctgaatgct caacagttag atgaaataaa ggactttggc cgctacttgg atgtggacgg
     3301 cgacggcatc ccttaccgca ccattccggg cacgcatcct accaaaggcg ctttcaccac
     3361 gcgcggcacc tcccgcgacg agtacgcccg ctacacggag gatgcagccg cctacgagca
     3421 ctccatcagg cgtttggata agaagtggaa aacggcctct actttggttc ccaagccgga
     3481 ggtgtaccgc tcgcgggggc acgcgctggg catgatctgg atcggcactt cgcaccacgc
     3541 tgcggtggag gccattgacc tgctggaaca acaaggcatt gcgatggacg gactgcgcgt
     3601 gcgggctttc cccttcaacg aaacggtgga ggctttcctc gatgaacaca agctggtgtt
     3661 tgtaatcgaa caaaaccgcg ataagcaatt ccgcacgctc ttggtgaacg agttggaaac
     3721 ggcgcccaaa aagctcgtgc ccatccaaaa cttcgatggt atgcccatca cggcagattc
     3781 gattttgaag caggtgctgg agagggtagg ggaggcagag gtgagcagat agctgcgaac
     3841 tgcgagtggg ccaggcgagc gggaaatttg acaaatcttg aagatttgtc aaattgtgct
     3901 gtgaactgac tcagcaagcg ggcgagcgag gtagtatgtc acctgaaaac gcaagatgac
     3961 ggcggaaaaa gtacaaaaca acaacaaatg gaacctgtaa ccacatccaa aatggtcggc
     4021 gcggtggccg gctacttagc gcaaacgctg aaagaccagc gctctgtgca ggattttttc
     4081 agtgacttca ccgacgcggc cgttcagtgg gtgcgcccgc tttttctgac cgacgagggc
     4141 caacccaaag aagtcatcga agacctgcaa aaagacccgg aagagaaact caataccgat
     4201 gcggtggaaa acaccctcgc caaagcgttg aaaaaagacc cttccgcaga ggcgttgctc
     4261 cagaccatgt acgaaaccat acaggccaaa gctgccgccg ggcaaaccat ctccatcgta
     4321 cacagcaaaa acgtagtgac cggcaatatc tcgacgaccg ggaatgtgat tattggcgac
     4381 aacaactctt gatacaagat gagcgagatt tcccacagca aaaatatcgt gaccgggcac
     4441 attgatgctc agggcaacgt cgttgtgggc gataccatca tcaacctcaa agaagctgcc
     4501 caatacaaag ccatagaagc ggagatagcc gagctgaaca cccgctttga acgcacccga
     4561 aagcgaatag aaaaagcccc tgacgacatg gatttccaga tggaattgct ggaaatagac
     4621 gccaagcgca gccaaaagca gcaggaattg gactcgctcc ggagggaagt tttgaaattg
     4681 gccgaagact tccagcgcat cccgctcaat accgagcgat tgcgccgggc gaaagcccat
     4741 ttcgagcagg gcgagttttc cgaagctcgt gctgtgctgg acgccgaagt cgaaacgatg
     4801 ggcattgaat tggacgcttt gctacatgaa aaagggcgtt tgaaccaaaa aacggctgaa
     4861 aaccaacaac atttgagcga caaagccaac gaatacctca tactcgcacg cctcaccgct
     4921 acggattacg aactgcccgg tcgttttgaa aaaacgacag cgcactttga gcagtccctg
     4981 cgggcagagc gaaatgcgga gaatgtgttt gcttttgccc attttttaca ggagcacaat
     5041 gtgttgatgc aggcacagcc cct
//
LOCUS       DAORLF010000358         5037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9092_length_5037_cov_1.878563, whole genome shotgun sequence.
ACCESSION   DAORLF010000358 DAORLF010000000
VERSION     DAORLF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_5037_cov_1.878563"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK198_21300"
     CDS             <1..493
                     /locus_tag="PK198_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41349.1"
                     /translation="VMPVSGVSFFRISTPSDYRTKLIIYTSRTHEVSWMLVHDVSDTF
                     QVSMVCDTLGQRLYQYDYCSRKDLNFGSIWVMENNTFQKVSKVEIPTLNKSLSNELID
                     RIELTKMNVSRSGLFAIFNYQNYLDEPPIEIKNVHMKLQQNRKGISTNFSVNHDFGIN
                     VLD"
     assembly_gap    548..607
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    846..883
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <884..1707
                     /locus_tag="PK198_21305"
     CDS             <884..1707
                     /locus_tag="PK198_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41350.1"
                     /translation="FTLYRLDFKALILDENGRKETVLIELQKSKLPTNVLRFRSYLGL
                     AYTQKIRAKDPETQVEHDSALPIISIYILGYNVEDIPHVAVKVNRKIMDMSSQQELDL
                     QSDFIDLLTHTTCILQVRRLPKERRSRIEQFLTLFNQAWVMEEKYILDLQEVPEGFED
                     IAEYLQRPLQEEEIRAKLRAEEEVEAIFAQQEADKAYYKRTAEEAKAREEEAKAREEE
                     ERRQKEEAKAREELALEQARHARIKLARLLLRTGMSVKDAALETGLSEEELKKLE"
     gene            1711..3018
                     /locus_tag="PK198_21310"
     CDS             1711..3018
                     /locus_tag="PK198_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41351.1"
                     /translation="MYKSLLTPAVLFLALSSVFAQSYTSFFTGSAANISTNPTGGICI
                     MGGATENDNAMRWFLQRAAGGDVLVLRASGSSGYNNYLYSELGVPVNSVETIVFNNAS
                     AAFDPYVHQRIAQAEAIWFAGGDQWRYVSFWRNTPVDSLVNVAIQNRNIVIGGTSAGM
                     AILGGFYFTAQNGTVTSGDALANPYNTRVTVDSAKFINTPFLNHVITDTHYDNPNRKG
                     RHAVFLARIFKDYGIEAKGIACDEYTAVCIDPAGGAAVYGNFPTSDDNAYFIQTNCEI
                     EERGPETCTSGTPLIWNRNGTALRVYKVKGTQNGANRFRLSDWKTGIGGVWEQWYVQG
                     GVVSETMGSPINCLTTSTNNNHDLESSLNIYPNPGNETLTIEAPRLIRKVQVLAYTGQ
                     IIHSQEGDASESLLLNTEKLPLGMYLLKIYTDNGDFVRQWVKI"
     gene            3330..3911
                     /locus_tag="PK198_21315"
     CDS             3330..3911
                     /locus_tag="PK198_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF41352.1"
                     /translation="MKVTPLNDQQLIASYLEGNEQAFEELLNRHQQKIYTSIYLFVKD
                     QSLAEDIFQEVFIKIIDTLRKGKYNHEGKFLQWAMRISYNMCVDYFRRNKRRPHVSTT
                     DTFDIFDVLQAPEYNAEQTIIRSQTHEKIRALVDMLPPEQREVVILRHYADMSFKEIA
                     KLTRVSINTALGRMRYALINIRKMVEEKEVILQ"
     gene            complement(4161..>4671)
                     /locus_tag="PK198_21320"
     CDS             complement(4161..>4671)
                     /locus_tag="PK198_21320"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF41353.1"
                     /translation="EYEYNRDIVQRIREALDKHDVKYYITVPEVDIDDFLKGRVDRAN
                     DLKSDLPKIFVSVHGNAGPAANSETWSPASGIETWFFHGSARSQKIASIFQKHLIEKT
                     GFRNRNIKSQPTGQFYVLRNTNMPSVLTENGFYNNKVEGAEMMKPEVRQKIADAHVAA
                     ILEIEKSGL"
     assembly_gap    4672..4727
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4728..>5037)
                     /locus_tag="PK198_21325"
     CDS             complement(<4728..>5037)
                     /locus_tag="PK198_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41354.1"
                     /translation="VSEMEKVIIPPNEPDEAFVKDIFEEAPAPPVNPPTPTTPTTPTP
                     PATTGGGTAAPTTTTPATGGGATSPTTPTTPTTTTPPKPAAPASKGRFMWCLDNGHGK
                     K"
BASE COUNT         1323 a         1137 c         1231 g         1192 t
ORIGIN      
        1 agttatgccg gttagcggag tttctttttt caggattagt acgcccagtg actatagaac
       61 aaaattgatc atttatactt ctcgcactca cgaagtatca tggatgctgg tccatgacgt
      121 atctgacact tttcaggtat ctatggtctg tgatacgttg ggacaaagat tgtatcaata
      181 cgactactgt agcagaaagg atttgaactt tgggagtatc tgggtaatgg aaaataatac
      241 ttttcaaaag gtttcaaaag ttgaaattcc aacattgaat aagtctttat ctaatgaatt
      301 gattgacagg atagaactaa caaaaatgaa cgtatcccgt tcagggttat ttgctatctt
      361 caactaccaa aactatcttg atgagccccc catagagatc aaaaacgtcc atatgaagtt
      421 acaacaaaat agaaagggca taagtacaaa ttttagtgtg aatcacgatt ttggcattaa
      481 tgtattggac tgaccttgcc agagtcttct ctcatccaaa aaacaaaaaa acacaatcgc
      541 tggtaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnncgt aggaaaaaaa acgccactac ctcggcggct tagagttcaa aaacgacttg
      661 ccggaagcct acaactttgg cgacggccgc gttgtttttc gcgtacaata cagtaacttt
      721 gcctgatgaa aatcgccaat ccgctatacg atcacgcctt caagtacctg atgtcgaacg
      781 acaagctggc ccgcaaggtg ctgtccgtca ttctggaaaa ggaagtactg gaactggaac
      841 tctccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttcac cctgtatcgt
      901 ttggatttca aggccctcat cttggatgaa aacggcagaa aggaaaccgt actcatcgaa
      961 ctgcaaaaat ccaagctgcc caccaatgtg ttgcgttttc gcagttatct cggcttggcc
     1021 tacacacaaa aaataagggc caaagacccc gaaacacagg tagaacacga cagcgccctg
     1081 cccatcatct ccatctatat actggggtac aatgtagaag acattccgca cgtcgccgtc
     1141 aaggtgaacc gcaagatcat ggatatgtca agccagcagg agttagacct acagagcgat
     1201 tttattgact tgctcacgca tacaacgtgt atcttgcagg tgcgccgcct gcccaaagag
     1261 cgccgcagcc gcatcgagca atttctcaca ttattcaatc aagcctgggt catggaagaa
     1321 aaatacatcc tcgatttgca agaggtgccg gaaggttttg aagacatagc ggagtaccta
     1381 cagcggccgc tacaggagga agaaatacgg gctaaactgc gcgcagaaga agaagtggag
     1441 gccatcttcg ctcagcagga ggcagataaa gcgtactaca agcgaacggc tgaagaagca
     1501 aaagcacgcg aagaagaagc aaaagcacgc gaagaagaag aacgccgcca gaaagaagaa
     1561 gcaaaagcgc gcgaagaatt ggcgctggaa caagcgcgcc atgcccgaat caaattagcc
     1621 cgcctgctgc tgcgcacggg tatgtccgtc aaagatgcgg cgcttgaaac ggggctatcg
     1681 gaggaggaac tgaaaaaatt ggaataatct atgtataaat ctttactcac cccggcggtg
     1741 ctgtttctgg cgctgtcatc tgtttttgct caatcctaca cgtcattttt cacggggagt
     1801 gcagccaata tcagcaccaa tcccacggga ggcatttgca ttatgggcgg agctaccgaa
     1861 aacgacaatg ccatgcgctg gtttttgcag cgggcagccg gcggcgatgt gctggtgttg
     1921 agagcaagcg gttcgagtgg gtataacaac tatctgtaca gtgaattggg agtgccggtc
     1981 aattcggtag agaccattgt tttcaataac gcatcggcgg cttttgatcc ctatgtgcat
     2041 cagcgcattg cacaggccga ggctatctgg tttgcagggg gcgatcagtg gcggtatgtt
     2101 tccttttggc gaaatacgcc ggtggacagc cttgtcaatg tggccattca aaaccggaat
     2161 atcgtcatag gcggcaccag cgcaggtatg gccatactgg gtggttttta tttcacagct
     2221 cagaacggta cggtaacatc gggcgatgca ctggcgaatc cttacaatac gcgcgtcaca
     2281 gtggattcgg cgaaattcat aaacactcct tttttgaatc atgtcattac cgatacgcac
     2341 tacgacaacc cgaatcgcaa aggccgccac gccgtttttc tggcgcgcat ctttaaagat
     2401 tatggtatag aggccaaggg cattgcctgc gacgaataca ccgccgtatg tattgacccc
     2461 gccggtggcg ctgcggttta tggaaatttc cccacctcgg atgacaatgc ctatttcata
     2521 cagaccaact gcgaaatcga agagcgcggc ccggaaacct gcacttcggg aaccccgctg
     2581 atctggaaca gaaacggaac tgcgctgcgg gtttacaaag tgaaaggcac ccaaaacggc
     2641 gccaaccgct ttcggctcag cgactggaaa acgggcatcg gcggtgtatg ggagcagtgg
     2701 tatgttcaag gaggcgttgt atcagagacc atgggaagcc cgattaattg tctgactact
     2761 tcaacgaaca ataaccatga tctggaatcg tctctgaaca tataccccaa tccgggaaat
     2821 gaaacgctga ccattgaggc gccgcgtctc atccgaaaag ttcaggtctt ggcctatacc
     2881 ggccaaatca tccatagtca ggagggagat gcctccgaat ctcttctttt gaacacggaa
     2941 aaactaccct tgggaatgta tcttttgaag atttatacag acaacggcga ctttgttcgc
     3001 caatgggtga aaatataata acagggtagg caacagaggc taatttattg taaactgttc
     3061 aaaattaaat atttgtacaa agcgggggcg tcattgaaac gaattttgcg actaaaaaaa
     3121 ttgttttcat ttcgttaatt tcttgttaaa gcccgaaaaa agagacatta aaatgacgca
     3181 gaattgaaaa acccgcttaa ctttggtgca tcgttttcaa cgaagaaggc aaatttttat
     3241 cattttacca aaaacgctcg tctatcgaaa gtaacttttt acactaatta agttcgtact
     3301 tttttttgtc gtactaaaac tttttagtta tgaaagttac gccgctcaat gaccagcagc
     3361 tcatcgccag ttacctcgaa ggtaacgagc aagccttcga ggaactcctc aatcgccacc
     3421 aacagaagat ttacacttcg atctatctct ttgtgaaaga tcaaagtctg gctgaggata
     3481 tttttcagga agttttcatc aaaatcattg atacgctccg aaagggcaaa tacaaccacg
     3541 agggcaagtt ccttcagtgg gccatgcgca tttcgtacaa tatgtgtgtg gactatttcc
     3601 gccgcaacaa gcggcgccct catgtttcta caaccgatac cttcgacatt ttcgatgtgc
     3661 tgcaagcgcc ggaatacaat gctgagcaaa cgattatccg cagccagacg catgaaaaaa
     3721 tccgtgcttt ggtggatatg ttgccgccgg agcagcgcga ggtggttatc ctgcgccact
     3781 atgccgatat gagctttaag gaaatagcca agctgacccg cgtatctatc aatacggcat
     3841 tgggtcgcat gcgctatgca ctcatcaata tccgcaaaat ggtggaagaa aaagaggtta
     3901 ttctgcaata atattcagtc gaaaaaacac acaacgcgag aggcgatatc ctgatgttca
     3961 gggtatcgcc tcttgtttta ggaggatgtt acagcagggg gcgaaatgga taccgccctt
     4021 aaaggcggta tggaatggag cacccctgac tgccgtcagg caggcggcgc ccgttgcggg
     4081 tgagagctgt cagctttggc aggctgttgc ggggataaag ctaatagctc tttacaggag
     4141 cgggcggctg ccgtaaaaaa ttacaagccc gacttctcga tctccagaat ggcagctaca
     4201 tgcgcgtcgg caattttctg gcgcacttcg ggcttcatca tttcggctcc ttcgactttg
     4261 ttgttgtaaa agccgttttc ggtcaatacc gacggcatgt tggtgttgcg caaaacgtag
     4321 aactggccgg tgggttgcga cttgatgttc cgatttctaa atcccgtttt ttcgatgagg
     4381 tgcttctgga aaatagacgc tattttctgg ctgcgcgcgc tgccgtggaa gaaccaggtt
     4441 tcgatgccgg aagccggact ccatgtctcg gaattagctg cggggccagc attgccatgt
     4501 acagagacaa agattttcgg cagatcggac ttgaggtcgt tggctcggtc cacccggcct
     4561 ttgaggaagt cgtcaatgtc cacctccgga accgtgatgt aatatttgac atcgtgctta
     4621 tccaaagcct cgcggatgcg ctgcacgatg tcccggttgt attcatattc annnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt tttgccgtgg
     4741 ccgttgtcta agcaccacat aaagcggcct ttggaagcag gggcagcagg tttgggcggc
     4801 gttgttgtag ttggcgtggt aggagttgtg ggtgatgtgg cgcctcctcc ggtagccggc
     4861 gttgtggttg tcggagccgc agtacctccc ccggtggtag ccggcggcgt aggggttgta
     4921 ggagtcgtgg gagtgggcgg attgaccggc ggtgcagggg cctcctcaaa aatatctttc
     4981 acaaaggctt catccggctc attgggcgga atgatgactt tttccatttc actgaca
//
LOCUS       DAORLF010000359         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9100_length_5033_cov_2.167939, whole genome shotgun sequence.
ACCESSION   DAORLF010000359 DAORLF010000000
VERSION     DAORLF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9100_length_5033_cov_2.167939"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK198_21330"
     CDS             <1..794
                     /locus_tag="PK198_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRF41355.1"
                     /translation="RLLSAVMKEQQLKIYKEHGLESYLAEVKKDESSTAWDRLIKSWT
                     KATPVAKEQDVMLDHNYDGIRELDNSLPPWWVALFYITIAFAVVYMAYYHFSGSGPSS
                     KDEYDIQMAKAQEEVDLYLSKQANKVDENSVVVLTDANEIALGKTVYDANCVACHGAA
                     GEGGIGPNMTDDYWIHGGSIKDMFKIIKNGVLEKGMQAWGSQLRPVDMQRVASYIMTM
                     RGTNPPNGKAPQGDLYKEEAAPAEGAAPAEGAAPAQEAAPAEGQQ"
     assembly_gap    1197..1220
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1221..2477
                     /gene="ccoG"
                     /locus_tag="PK198_21335"
     CDS             <1221..2477
                     /gene="ccoG"
                     /locus_tag="PK198_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRF41356.1"
                     /translation="NVLERKFILFGIVFMPQDFHLFGLAMLTLVVFIVLFTVVFGRLF
                     CGWVCPQTIFMEMVFRKIEYWIEGDAAAQRKLKAAPWTSEKIIKKTAKQVIFFLIAIL
                     VSNTFLAYIIGVDEVIKIATEPVSQHLGGFIAMVLFSFAFYFVFSYLREQVCVAICPY
                     GRLQGVLLDRNSIVITYDHVRGEPRGKIRKEKKTAGSPVSDIAASVTEAAAEAKPLGD
                     CIDCKLCIHVCPTGIDIRNGTQLECVNCTACIDACDEVMDKVNRPRGLIRYDSHNGIV
                     EQRRRIFTPRVIAYTVFLSLLLVLNFAFLSGRTEVETIILRTPGLMYQKVDELHISNL
                     YNYEVINKTSNDLPVELRSITEGAEIKLVGKAPIVKAGDVGKGGMFIIMEKSKTNGRK
                     NSVVIEVISNGKVVDKVKTNFFAPPK"
     gene            2589..3029
                     /locus_tag="PK198_21340"
     CDS             2589..3029
                     /locus_tag="PK198_21340"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRF41357.1"
                     /translation="MKLNWGTGIALFYGTFVVVLVAVVIKSTTFDNSLVSEQYYADDL
                     NYQQQYNKIANSLDLTNDLSVTTQAAEQQVELLFPTEVGQASGEIYFYNPSYKAGDFR
                     VAVQPDSNSRQVVSTAALRPGLWRVKVDWQAGGKNYFKEVVITI"
     gene            3034..3735
                     /locus_tag="PK198_21345"
     CDS             3034..3735
                     /locus_tag="PK198_21345"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF41358.1"
                     /translation="MGVLFSAFGIGLVGSLHCIGMCGPIALALPYQQGSRLHALGRVL
                     LYNTGRVLTYTVLGTVIGLAGRGLFLAGIQSWMSIALGVLFLIAALFSINLESRIVRL
                     PGIHQLNAWVQSTLGRLLRRQDSASLLGIGALNGLLPCGLVYMAVAGAVTSGSAAQGA
                     AFMALFGLGTVPMMAAVAMAGQFISLRWRNRVRKLLPVFLITFALFFIARGLNFTVPA
                     DIRFWETMQDQPMCH"
     assembly_gap    3839..3911
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3954..4982
                     /locus_tag="PK198_21350"
     CDS             3954..4982
                     /locus_tag="PK198_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF41359.1"
                     /translation="MIKTDIAIIGAGPVGLFTVFEAGLLQMHCHLIDVLGTPGGQLAE
                     IYPKKPIYDIPGYPSVLAHELVDNLMQQIAPFNPTFTLGERAETLERLEDNSFLLTTN
                     KGTQINAPVVAIAAGLGSFEPRKPPIDNIADFEDSGVEYIVKDPELYRNKRVVIAGGG
                     DSALDWAIYLADVAKELYLVHRRTEFRGAPDSVEKAMHLAHEQRITLITQAQVTGLNG
                     NGRLEEVVIEHDERGQFSVATDHFVPLFGLTPKLGPLENWGLDVDKNGIKVDTFDYQT
                     NVPGVYAIGDINYYPGKLKLILCGFHEATLMCQSAFKIVNPEKKLSFKYTTVTGISGL
                     EQGTPQTA"
BASE COUNT         1202 a         1372 c         1252 g         1110 t
ORIGIN      
        1 cgcgcctgct cagcgctgta atgaaagagc agcaactcaa aatttacaaa gagcacgggc
       61 tggaatctta cttggccgaa gttaaaaaag atgagagcag caccgcttgg gaccgcctca
      121 tcaagagttg gacgaaagca acgccggtgg ccaaagagca ggatgttatg ctcgatcaca
      181 actacgacgg cattcgggaa ttggacaaca gccttccgcc ttggtgggtg gccttgttct
      241 acatcaccat tgcttttgca gtagtgtata tggcttacta ccacttctcc ggctccggcc
      301 cgtcttccaa agacgaatat gatattcaaa tggccaaagc tcaggaagaa gtggacctct
      361 acctctccaa gcaagccaac aaagtggatg agaacagcgt ggtggtactc accgatgcca
      421 acgaaattgc gctgggcaaa actgtttacg atgccaactg cgtagcttgc cacggagcag
      481 ccggcgaagg tggcattggc ccgaacatga ccgacgacta ctggattcat ggcggcagca
      541 tcaaagacat gttcaagatc atcaaaaacg gcgtgctcga aaaaggtatg caggcttggg
      601 gcagccaatt gcgtccggtg gacatgcagc gcgtagccag ctacatcatg accatgcgcg
      661 gcaccaatcc gccgaatggc aaagcaccgc aaggtgatct gtacaaagaa gaggcagctc
      721 ctgctgaagg cgcagctcct gccgaaggtg cagctcccgc acaggaagcc gctcctgccg
      781 aagggcagca gtaatatcca tttttctcgc agagctgtta gctttggcct gttacaactc
      841 gctaaagcta acagctctac tcccgcctac tgcccacttc cttccctatt tccttcgtcc
      901 atcgggacag gctctacgtc atcgggagga cttcgcttcg ctcgactgca caccgcctac
      961 cgcacaccga aaccccaccc cattcacatc atgaacgcct gaaaaagaaa cttttaaaac
     1021 acgaccatgg atccgataga agccatctac gataccgagg agtatcggga ccgcattgcc
     1081 accgttgacg agcaaggcaa gcgcgtatgg atttatccca aaaaacctaa aggcagattt
     1141 acgaatatgc gcacagtgtt gagctgggtg ctgttggtat ttttatttgg gatgccnnnn
     1201 nnnnnnnnnn nnnnnnnnnn aatgtattgg aacgaaaatt tatcctgttc ggcattgttt
     1261 tcatgccgca ggatttccac ctgtttgggc tggccatgct cacattggtg gtgttcattg
     1321 tgttgttcac ggtagttttc gggaggttgt tttgcggctg ggtttgccca cagaccattt
     1381 tcatggaaat ggttttccga aaaatagaat actggattga aggcgacgcc gccgcccagc
     1441 gcaaactcaa agccgcgccc tggacttcag aaaaaatcat caaaaaaaca gccaagcagg
     1501 tcatcttttt cctcattgcc atattggtat cgaatacttt tctggcctac atcatcggtg
     1561 tggacgaggt gattaagatt gctaccgaac cggtgagcca acacctcggc gggttcattg
     1621 caatggtgct gttttcgttc gcattttatt ttgtcttttc ctacctgcgc gaacaagtat
     1681 gcgtggctat atgtccgtat ggccgtttgc agggcgtact gctcgaccgc aactccatcg
     1741 tcatcactta cgatcatgtg cgcggcgaac cccggggcaa aatccgcaaa gagaaaaaaa
     1801 ctgccggttc gccggtttca gatattgctg cctccgtcac cgaagccgct gccgaagcca
     1861 aaccgctcgg agactgcatt gactgcaaac tctgcatcca cgtctgcccc acaggcattg
     1921 acatccgcaa cggtacgcaa ttggagtgcg tcaactgcac ggcctgcata gacgcctgcg
     1981 acgaggtaat ggacaaagtg aaccgcccgc gtggcctcat ccggtacgac agtcacaacg
     2041 ggatcgtgga gcaacgccgc cgcatcttta cgcccagagt gattgcttat acggtatttt
     2101 tgtcgctgct gctggtcctg aatttcgcat tcctcagcgg acgcaccgag gtggaaacca
     2161 tcattttgcg cacgcccggc ctcatgtatc aaaaagtgga cgagctgcat atcagcaacc
     2221 tctacaacta cgaggtgatc aacaaaacca gcaatgactt gcccgttgag ttgcgcagca
     2281 tcaccgaagg ggccgaaatt aaactcgtag gaaaggcccc tattgtcaaa gcaggcgacg
     2341 tgggcaaagg cggaatgttc atcattatgg agaaatccaa aaccaacgga cgcaagaata
     2401 gtgttgttat cgaagtgatt tccaacggca aggtggtgga caaggtgaag accaatttct
     2461 tcgctccgcc gaagtaaagg gaggcggttc aaggttccgg gttcaagatt tctggttgca
     2521 ccgcctactg agaactgcaa accgcctact gaaaaccgcc taccgcaaac tgctaaaata
     2581 cgctgaacat gaaactcaat tggggaaccg gcatcgctct tttttacggc acatttgtag
     2641 tcgtactcgt agcggtcgtc atcaaatcaa ctactttcga caacagcttg gtatctgaac
     2701 aatactatgc tgatgatctc aactaccaac aacagtacaa caaaattgcc aacagcctcg
     2761 atctgaccaa tgatctgagc gtcaccacac aggctgccga gcagcaggta gaactgctct
     2821 tccctactga agtgggtcag gcctccggcg agatttactt ttacaatcct tcctacaaag
     2881 ccggcgattt ccgcgtggcc gttcagcccg acagcaacag ccgccaggtg gtctctaccg
     2941 cagcattgcg gcccggcctc tggcgtgtaa aagtggactg gcaagccggc ggaaagaact
     3001 attttaaaga ggtggtcatc acgatctgat ctcgtgggcg tactcttctc cgcattcggc
     3061 atcggcttgg taggcagtct gcactgcatc ggcatgtgcg gccccattgc cttggccctg
     3121 ccctaccaac agggcagtcg cctgcacgcg ctggggcggg ttttgctcta caatacaggc
     3181 cgggtactca cttacaccgt tctcggcacc gtcataggtt tggcgggacg aggtcttttt
     3241 ctggccggca ttcagtcgtg gatgtccatt gcactggggg ttttgtttct catcgccgcc
     3301 cttttttcca tcaatttaga gagcagaatc gtgcgcctgc ccggcattca tcaactcaat
     3361 gcctgggtgc aaagtacgct gggccgtctg ctgcgccgac aagacagcgc ctctctgctc
     3421 ggcatcggag cgctcaatgg cttgttgcct tgcggcttgg tgtatatggc cgttgcgggt
     3481 gccgtcacat cgggcagcgc agcgcaagga gccgcattca tggctttgtt tggcttgggc
     3541 accgttccca tgatggcggc agtagcgatg gccgggcagt tcatcagcct gcgttggcgc
     3601 aatcgggtgc gaaaactttt gcccgttttt ctcattacct ttgcgctgtt tttcatcgca
     3661 cgcggtttga acttcacagt tccggcagac attcgcttct gggaaaccat gcaggatcag
     3721 ccgatgtgcc attaagtatt tcggattcct gatttcggat tgtgcagata tacgttcgcg
     3781 gaaattcgca ctattcgcta aaattcgcgt tcaaaaaata ttcgcgttcg cgtttctgnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn ncagcttgag gccgatacaa caggaagtag taaccacaca aaaatgataa
     3961 agacagacat cgccatcatc ggcgcaggcc ccgtgggtct gttcacggtt tttgaagcag
     4021 ggcttttgca aatgcactgc cacctcatag acgtactcgg cacgcccggc ggccaattgg
     4081 cggaaattta ccccaaaaag ccgatttacg acatccccgg ctacccatcg gtgctggcac
     4141 acgagttggt ggacaacctc atgcagcaaa tcgctccctt caaccctacc ttcacgctgg
     4201 gcgaacgcgc cgaaacgctc gaacgcctcg aagacaattc cttcctgctc accaccaaca
     4261 aaggcacgca gattaatgcg cccgtagtgg ccatcgctgc cggcctcggt tccttcgagc
     4321 cgcgcaagcc gcccattgac aacatcgccg attttgaaga cagcggcgta gagtacatcg
     4381 tcaaagaccc ggaactctac cgcaacaagc gcgtcgtcat tgccggcggc ggagacagcg
     4441 cactcgactg ggccatttac ctggctgatg tagccaaaga actgtatctg gtacaccgac
     4501 gcaccgaatt tcgcggcgct cccgacagcg tggaaaaagc aatgcacttg gctcatgagc
     4561 agcgcattac gctcatcacg caggcgcagg tgaccggcct caacggcaat ggacgcctcg
     4621 aagaagtcgt tattgaacac gatgagcggg ggcagttttc ggtagctacc gaccactttg
     4681 tgcccctttt cggcctcacg cccaaactcg gcccgctgga aaactggggc ctcgatgtgg
     4741 acaaaaatgg tatcaaagtg gacacatttg actaccaaac caacgtaccc ggcgtatatg
     4801 ccatcggcga catcaactac tatccgggca aactcaagct cattctctgc ggcttccacg
     4861 aggcgacgct tatgtgccaa tcagctttca aaattgtcaa tccggagaag aagctcagct
     4921 ttaaatacac caccgttacc ggcatcagcg gcctcgaaca gggtacgccg caaactgcct
     4981 gagtagtggg gcaaaaataa atgctcattt tctttaggcc aaaggattag ata
//
LOCUS       DAORLF010000360         5028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9123_length_5028_cov_1.825860, whole genome shotgun sequence.
ACCESSION   DAORLF010000360 DAORLF010000000
VERSION     DAORLF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9123_length_5028_cov_1.825860"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1230)
                     /locus_tag="PK198_21355"
     CDS             complement(<1..>1230)
                     /locus_tag="PK198_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRF41360.1"
                     /translation="MGYDGSVYLMGWTGGYVLLALLLAPYLRKFGKFTVPDFIGERYY
                     SQTARIVAVVCALFVSFTYVAGQMRGVGIVFSRFLEVDISLGVIIGMCIVFFYAVLGG
                     MKGITYTQVAQYVVLIFAYMVPAIFISFLMTGNPIPQLGLGGTTTDGGYLLDKLDGLN
                     QELGFAAYTSGTKSMIDVFAITAALMLGTAGLPHVIVRFFTVPKVKDARSSAGWALLF
                     IAILYTTAPAVAAFVRTNLIETVSNQPYSQMPEWFSKWEEAKLIAFDDKNKDGLIQYV
                     KDPAVNELTVDKDIMVLANPEISKLPNWVIGLVAAGGLAAALSTAAGLLLVISSAISH
                     DLLKNAINPNISEKGELRAARIAAAAAVVMAGYLGVNPPGFVAEVVAFAFGLAAASFF
                     PAIILGIFDKRTNMPGA"
     assembly_gap    1231..1257
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1258..1446)
                     /locus_tag="PK198_21360"
     CDS             complement(<1258..1446)
                     /locus_tag="PK198_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRF41361.1"
                     /translation="MSVQIWTLLIVGLSFALYIGIAIWSRASSTKEFYVAGGGVNPLM
                     NGMATAADWMSAASVISMA"
     gene            complement(1537..1791)
                     /locus_tag="PK198_21365"
     CDS             complement(1537..1791)
                     /locus_tag="PK198_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRF41362.1"
                     /translation="MSGKDLKKYWKENVTYLLILLGVWFLVSYGAGILFVKQLDSIRI
                     GGFKLGFWFAQQGSIYTFLVLIVVYVRLMNKLDKKYDVNE"
     gene            complement(1831..>2963)
                     /locus_tag="PK198_21370"
     CDS             complement(1831..>2963)
                     /locus_tag="PK198_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41363.1"
                     /translation="AYAQISPRFLVLTHWGINNQSFINGATAPNGPNATTSPSNQGKK
                     PSVFIHDAWTEYQVVKDKLYLGAGLHYWNGVSRFTSHSTLNFMATDAPIFNWYNIETT
                     DQFARQFGFYAKGQLGRLDYRFHLNKPFVNGPVASKTNTSANAINVASETWAPGGYVN
                     WMFFDKESNKLPFYVGSYIGAKKVFNLGAGFYSQTEATASRSIVGQDTTVNRHNQLCL
                     GVDAYLDMPIKGGKGGAAHAYLVYYNYDFGPGYLRNIGILNEHLSVDAAKPDRSWAGG
                     GNAQPTIGTGSIVYGQFGYALPKNADGTQWMPFASVTQKNFERLSEASLQYSLGLNYF
                     INAHHAKVTLEYQTRPIYQLNGTAIERNGSKGQFILQTQIFL"
     assembly_gap    2964..3006
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3007..3252)
                     /locus_tag="PK198_21375"
     CDS             complement(<3007..3252)
                     /locus_tag="PK198_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41364.1"
                     /translation="MTNKKQRWIGSLILGLLFCVSGYSQDAEKPKVDHSYKPLTLKLS
                     EDGNKYVRLLIWHQFWATLNQNNPGTLDVNGNEINTTT"
     gene            3632..>3831
                     /locus_tag="PK198_21380"
     CDS             3632..>3831
                     /locus_tag="PK198_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41365.1"
                     /translation="MAAPSVKIPTNYTHNLNFQNGLTISVFAMNVKSESLVVGDKARP
                     VATLLLGDPTQPLTTTIQGNKD"
     assembly_gap    3832..3841
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3842..4145
                     /locus_tag="PK198_21385"
     CDS             <3842..4145
                     /locus_tag="PK198_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41366.1"
                     /translation="VVSHVDLLNLPHLSKQDIKDLLTPEIRMRIPNYNQISFKFLGIE
                     LFNVCLSGESQVITEPYVPNAYERCETDCCEPDTRPFPQSSQGVIFSDFVNKPAGS"
     assembly_gap    4236..4246
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4247..>4637
                     /locus_tag="PK198_21390"
     CDS             <4247..>4637
                     /locus_tag="PK198_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41367.1"
                     /translation="NCGSSITGLSKELPMKQYGKEMPLQAGLSLNMPLSPGENRRQSL
                     CMTLPANTAPGTYRQKVEVGGEEKNLTMIVQETLEIELIPENLLFVGIAPGMSHTKEV
                     LLVNKGNVPFSVPVLRHNMSLDMDLVCR"
     assembly_gap    4638..4741
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4742..4954
                     /locus_tag="PK198_21395"
     CDS             <4742..4954
                     /locus_tag="PK198_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41368.1"
                     /translation="MTDWIEVRIKEAGTVVAPGESVVLHITFILPKDIHPNREYEGDL
                     RILDQLLSYMLIPGPKNDVVKKTTQV"
BASE COUNT         1225 a         1225 c         1214 g         1169 t
ORIGIN      
        1 atagcgcccg gcatgttggt gcgtttgtca aaaataccca gaatgatggc cgggaagaag
       61 gaggctgccg ccaaaccgaa ggcgaaggcc actacctcgg ccacaaaacc tggcggattg
      121 acacccaaat aaccggccat aaccaccgct gctgctgctg cgatacgggc cgctctcaac
      181 tccccttttt cggaaatatt gggattgatg gcattcttga gcaaatcgtg cgaaatggcc
      241 gatgaaatca ccagcaacaa acccgccgcc gtagaaagtg ctgccgccag accgcctgcc
      301 gctacgaggc cgatgaccca gttagggagc ttggagattt cagggttggc cagtaccatg
      361 atgtctttgt ccaccgtcag ctcgttcaca gccgggtctt tcacatattg gatcaagcca
      421 tctttatttt tgtcgtcgaa agcgatgagt tttgcctcct cccatttgct gaaccattcc
      481 ggcatctggc tgtaaggctg gttggatacc gtttcaatga gattggtgcg cacaaaagct
      541 gctactgccg gcgccgtagt ataaagaatg gcaatgaaga gcagcgccca acctgcagaa
      601 gaacgggcgt ctttcacttt aggtaccgta aagaagcgca caataacgtg cggcaaaccg
      661 gccgtgccca acatcagggc agcagtgatg gcaaagacgt ctatcatgga tttggtgccg
      721 ctggtatatg ccgcaaagcc caattcctga ttcagtccgt ccaatttgtc taaaagatag
      781 ccgccgtcgg tcgttgttcc gcccaagccc aattgcggaa tcgggttgcc ggtcatcagg
      841 aaagaaatga agatggcagg caccatgtaa gcaaagatca atactacata ctgtgccacc
      901 tgcgtgtagg tgatgccttt catgccgccc aatacggcgt agaaaaacac aatacacatg
      961 ccgatgatca cgcccaggga aatatccacc tccaaaaaac gggaaaacac gatgcctacg
     1021 ccgcgcattt gcccagctac ataggtaaag gaaacaaaca aggcgcaaac aaccgccacg
     1081 atgcgggccg tttgagagta gtaccgctcg ccgatgaaat caggcaccgt aaacttgccg
     1141 aattttcgca ggtagggcgc cagcagcaag gccagcaaca catagccgcc ggtccaaccc
     1201 atgaggtaaa ctgaaccgtc gtagcccatg nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
     1261 catagagata accgatgccg cggacatcca gtccgccgct gtagccatgc cgttcatcaa
     1321 gggattgacc ccgccgccgg ctacataaaa ctccttggtg gatgatgccc tcgaccatat
     1381 tgcgatgccg atgtacaggg caaatgacaa acccactatt agcaatgtcc aaatttgaac
     1441 gctcattgtt gttgattttt aaaatgaaaa aatgttgaaa aacttggctt gcggcttaca
     1501 gcctgtagct tgaagccgtt cccaactgcc cgcagtttac tcgttcacat catacttctt
     1561 atccaacttg ttcatcagcc ggacatagac gacaatcaaa acgaggaagg tgtaaatgga
     1621 gccttgctgg gcaaaccaga atccgagttt gaacccgccg atgcggatgc tgtccaattg
     1681 tttgacaaac aggatgccgg cgccgtagga caccaaaaac cagactccca gcagaatcag
     1741 caggtatgtc acgttttctt tccaatactt ttttaagtct ttaccactca tggttgctgt
     1801 gttttattgg tgagaaggaa atgagttgat ttacaggaat atctgtgttt gcagaatgaa
     1861 ctgccccttg ctgccgttgc gctctatggc tgttccgttg agttgatata tcggccttgt
     1921 ctggtattcc agtgttactt ttgcatggtg cgcattgatg aagtagttca gtcccaggct
     1981 gtactgaagg gaggcctcgg acaatcgctc gaagtttttc tgagttacgg aggcaaacgg
     2041 catccattga gtaccgtctg cgtttttggg caaggcataa ccaaattggc catacacaat
     2101 gctacctgtg ccgatcgtgg gttgcgcatt gccgccgccg gcccaactgc ggtcgggttt
     2161 ggcagcgtcc acactcaaat gctcgttgag aatgccgatg ttgcgcaggt agcccggccc
     2221 aaaatcgtag ttgtaataga ccaaatacgc atgtgcagcg ccgccttttc cgcctttgat
     2281 gggcatgtcc aaataagcat ccacgccgag gcaaagctgg ttgtgccggt tgacggtggt
     2341 gtcctggccc actatgctgc gcgatgcggt ggcctctgtc tggctgtaaa atccggcgcc
     2401 gaggttgaat acttttttgg cgcctatgta gctgcccaca taaaacggaa gcttgttgct
     2461 ctccttgtcg aagaacatcc agttgacgta gccgcccggc gcccaggtct cggaggccac
     2521 attgatggca ttggccgaag tattggtctt gcttgctacc gggccgttca caaagggctt
     2581 gttgaggtgg aagcgatagt ctaaacgccc caactgcccc ttggcataaa acccgaactg
     2641 gcgcgcaaac tggtcggtgg tttcaatgtt gtaccagttg aaaatcggcg cgtcggtagc
     2701 catgaaattg agcgtgctgt ggctcgtgaa gcgcgatacg ccgttccagt agtgcaggcc
     2761 ggcccccaga tagagcttgt ctttcaccac ctggtattcc gtccaggcat cgtggatgaa
     2821 cacggagggc tttttcccct gattgctggg agaagtggtg gcatttggcc cgttgggcgc
     2881 cgtcgctcca ttgatgaacg actggttgtt gatgccccag tgcgtgagta ccagaaacct
     2941 cggtgaaatc tgcgcataag ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnggtg gtcgtgttga tctcgttgcc gtttacatcc aaggtgccgg ggttgttttg
     3061 attgagcgta gcccaaaact gatgccagat caaaagcctg acatacttgt tgccgtcttc
     3121 gctcagtttc agcgtcagcg gcttgtagga gtgatctact ttgggctttt cagcatcctg
     3181 cgagtacccc gaaacgcaga acaacaatcc caaaatcaga ctcccaatcc atctttgttt
     3241 tttgtttgtc atacctgtgc aatttttggt tggttggaaa aatcgcacta aagtatatag
     3301 atgtttatat gtgtgcaagt atttcgataa ttttttttaa aaaaagtaaa aaatacagta
     3361 ttgttaatgg tttttatcct gatgctccgt attagatagg tggggttgta tgcagttttt
     3421 tagatgtgtt tcgttcccgt tgttcgagta tgccgcgata tgaagaatcg gccttttgca
     3481 tgctgtatca agccgaaccc acggcactta gaggagtgct cacacctgat gaagtttttg
     3541 ataattaaca tattgtgagg ataaaattta tgattacctg gattctctca ggaaaattct
     3601 atacatttgc catcaccaaa aactacatat tatggctgca ccttctgtca aaattcctac
     3661 caactacacg cacaatctca atttccaaaa cggcctcacc atttcggtgt tcgctatgaa
     3721 tgtgaaatcg gaatcgttag tagtcggcga caaagccagg ccggtggcaa cattgttgct
     3781 tggcgacccg acacaaccgc tcactactac cattcagggc aacaaagaca annnnnnnnn
     3841 ncgtggtatc tcatgtggac ctcctcaacc tgccccatct gagcaaacag gacatcaaag
     3901 acctgctcac gcctgaaata agaatgcgca ttcccaatta caaccagatc agtttcaagt
     3961 ttttgggaat tgaactgttc aatgtgtgcc tcagcggaga gtcgcaggtc atcaccgagc
     4021 cttatgtgcc caatgcctac gaacgttgtg aaacagactg ctgcgaaccg gacacccggc
     4081 ctttcccaca gtcgtcgcag ggagttattt tcagtgattt tgtcaataaa cccgccggat
     4141 catgatcatt gtgaattgcg aaactgcgca cgaaaaagtg cactgcttcg aaaaggaaat
     4201 tatcctcaac gggccgccct ctcaattgcg cgggcnnnnn nnnnnnagaa ctgcggctcc
     4261 agcatcaccg gactgagcaa ggaattgccg atgaagcaat acggcaagga aatgcccttg
     4321 caggcaggtt tgtcgctcaa tatgccgctc tcgcccgggg agaaccgccg ccaaagcctc
     4381 tgcatgactt tacccgccaa tacggctcca ggcacctacc gacaaaaggt agaagtcggc
     4441 ggcgaggaga aaaacctcac catgatcgtg caggaaacgc tggaaataga gttgattccc
     4501 gaaaatttgc tttttgtcgg cattgcgccc ggcatgtcgc acaccaaaga agttttgttg
     4561 gtcaacaagg gcaatgtgcc tttttcggtg cctgtgctgc ggcataacat gagcttagac
     4621 atggatttgg tttgccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 natgaccgat tggatcgagg tgcgcatcaa agaggcgggc actgtggtag cgccggggga
     4801 gtctgtagtg ctgcacatta ccttcatttt gcccaaagac atacatccca acagggaata
     4861 tgagggcgat ctgcgcattt tggaccaatt actgagttat atgctcattc ccggaccaaa
     4921 aaatgatgtg gtaaaaaaga caacgcaagt gtaaatcacc cccttaaacc agaataacat
     4981 ggattacctg caaactgctg aaaatctggt gcgtagcggc gccgaaga
//
LOCUS       DAORLF010000361         5026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9129_length_5026_cov_1.847918, whole genome shotgun sequence.
ACCESSION   DAORLF010000361 DAORLF010000000
VERSION     DAORLF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_5026_cov_1.847918"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK198_21400"
     CDS             complement(<1..234)
                     /locus_tag="PK198_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF41369.1"
                     /translation="MKKIKVVAVSYLNTKPLLYGLLRSPLAAQIELQLQIPSECARRL
                     QTGEADLGLVPVAVIPQLQTPHIISDFCIGTRGT"
     gene            complement(498..>1575)
                     /locus_tag="PK198_21405"
     CDS             complement(498..>1575)
                     /locus_tag="PK198_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRF41370.1"
                     /translation="EEGSLSFVGSLANYMREQRHGDNTYFNRNFHIEPTNLCVYDCKF
                     CSYSRLIKQRKDESAWEYTMEDMMDIVRSYDGQPVTEVHIVGGVLPQYDLPFYLDFFK
                     KIKAHRPELHVKALTPVEYHYIFKKAKVSYEDGMRMVAEAGCDSMPGGGAEIFHPEIR
                     EQIAADKCTGEQWLRIHEIWHELGRRSNATMLYGHIERFEHRVDHLEQLRQLQDKTGG
                     FQTFIPLKFRNQDNQMSHIPESTSVEDLRNYAISRIYLDNFEHIKAYWPMIGREVAQM
                     SLAFGVNDIDGTIDDTTKIYSMAGSEEQTPALSTRELAQLVRSVGRRPIERDTLYNVI
                     QDYTDHVFPEDQEFKGYLALPVVN"
     assembly_gap    1576..1585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1869..2162)
                     /locus_tag="PK198_21410"
     CDS             complement(1869..2162)
                     /locus_tag="PK198_21410"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41371.1"
                     /translation="MAALLGNVFIRSELYSTRSEICKEIKLHLDKSEVEEALYLLEKI
                     DEDEAVMLRSRWVARNKQSIRGVLSVDELDNGENKIKSAILDWVKLHETQALR"
     gene            complement(2125..>2874)
                     /locus_tag="PK198_21415"
     CDS             complement(2125..>2874)
                     /locus_tag="PK198_21415"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HRF41372.1"
                     /translation="DWSVEGVYRILDDQEVKARFGRFNEQDMERIWQPNRQDIHQGYH
                     FEEYFDELNELMRRFKLYYDLKYKDEHGYDRIAPSLLPKKQDSDADWGASPDDLRLQV
                     RYGFLPPALFSRFIVGMHRDIAGANRNHVWENEVWLEWENMEALAYLIEIENKKTIDI
                     RVRGKDLEKRKILLSFLLRELDEPQNNPPGIDRVKMVPCICEGCKTLEEPHFYALKDL
                     EEFKELDRAMQQVQKERSLRGGPARQRIHSQ"
     assembly_gap    2875..2938
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2939..4291)
                     /locus_tag="PK198_21420"
     CDS             complement(<2939..4291)
                     /locus_tag="PK198_21420"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41373.1"
                     /translation="MKDFLKKIFGDKPSEPQQESRQGEEAPLAHFEAIVSQDQPEPLS
                     PSVTSRYPKPEAVLQIEAFIRLPLAPADEYPSDELPGVRPHKENTPKYAVDEQQRLIG
                     LNLVKTGLTDAQWQSICRIPGALEHLRILNLSDNGGLTAFVFPPAPALRQLRILDLGD
                     CQLKQFELPAGGLPCLEDLELEGNPLEEIGPETWAGGKAQVLAFLKAGARKGFVKSLE
                     AKALLIGDGRCGKTTLRRRLLHGPEAPVVPSEERTKGLEVEVEPLWLSTAGGEKIRLN
                     LFDFGGQNHYKPLHQFFYSHKALYILVTKNGDDTNDWNFWCETANLYGGGSPVIIINN
                     LHDTEKSDFRGERVQKMEALVKEKYDCNLLRWYPEKQNASAQDKERLQRELGIIREKL
                     ATTVQELEHIHSTVPANWQEVRGILEGMRGRNIIPKTELEAICRRPEIDAEPEPCLEY
                     L"
     assembly_gap    4598..4777
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4778..>5026)
                     /locus_tag="PK198_21425"
     CDS             complement(<4778..>5026)
                     /locus_tag="PK198_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF41374.1"
                     /translation="EGNSGQKTHEVGQKYPNQLGLYDMSGNVWEWCADAYDEDFYKTC
                     AKSAGPIDNPFCKEGSMRVLRGGSFWSAPLRCAVAIRN"
BASE COUNT          961 a         1294 c         1316 g         1201 t
ORIGIN      
        1 tgtgccgcgc gtgccgatgc aaaaatcgga gatgatgtgc ggcgtttgta attgcggaat
       61 gacggctacc ggcaccaagc ccagatcggc ctcgccggtt tgcagcctgc gcgcacattc
      121 cgaggggatt tgcagttgca gttctatctg tgcagccaat ggacttctca gcaaaccgta
      181 caacaacggc ttggtattga ggtaactaac agcgactact tttatctttt tcatgttcat
      241 ttacttcgta aaaccggtgg gcggtaggca gtgggcggcg ctaagcgcga gggaaacccg
      301 aaacctggaa ccagtaggcg gtaggcggtg ctaagcgcga gggaaacccg aaaccttgaa
      361 cccgaaaccc gtccgttcct gccggaacgc caaaactttt aacgcgaatt ttagcgaagg
      421 acgcgaaatt ccgcgaacgc gagggaaacc cgaaacccgg aacctcgaac ccgaaacccg
      481 ccagttcctg ccggaactca attcaccacc ggcaatgcca aatacccctt aaactcctga
      541 tcttcaggga aaacgtggtc ggtataatcc tgaatgacgt tgtacaaagt atcgcgctca
      601 atcgggcggc gacccaccga gcggacgagt tgcgccagtt cgcgggtact cagcgcgggc
      661 gtttgttcct ccgagccggc catcgagtaa attttggtcg tatcgtcaat ggtgccgtct
      721 atgtcgttga cgccgaaagc gagcgacatc tgcgccacct cgcgcccgat catcggccaa
      781 taggctttga tgtgctcgaa attgtctaag tagatgcggc tgatggcgta attgcgcagg
      841 tcttccactg aggtgctttc cgggatgtgg ctcatttggt tgtcctgatt gcggaatttg
      901 agcggaatga aggtttggaa gccgccggtt ttgtcctgca attgccgcaa ctgctccaga
      961 tggtccacgc gatgttcgaa ccgctcgatg tggccgtaga gcatggtggc attggaacgg
     1021 cggcccaatt cgtgccagat ttcgtggatg cgcagccatt gctcgccggt gcatttgtcg
     1081 gcggcaattt gctcgcggat ttccgggtga aaaatctctg ccccaccgcc cggcatactg
     1141 tcgcaaccgg cctcggccac catgcgcata ccgtcttcgt agctcacttt tgcctttttg
     1201 aaaatgtagt ggtactccac cggcgtcagc gccttgacgt gcagttcggg gcggtgcgct
     1261 tttatttttt tgaaaaaatc gaggtaaaac ggcaggtcgt actgcggcaa cacgcccccc
     1321 acaatgtgta cctcggtgac gggctgcccg tcgtagctgc gcacgatgtc catcatatcc
     1381 tccatggtgt attcccaggc tgattcgtcc ttgcgctgct tgatgaggcg cgagtaggag
     1441 cagaatttgc agtcatacac acagaggttg gtcggctcta tgtggaaatt ccggttgaag
     1501 taggtgttgt cgccgtggcg ttgctcgcgc atgtaattgg ccaaggaacc gacgaaactg
     1561 agcgagcctt cttcannnnn nnnnntcctc gaacagcagg atgccctctt cgggtgtaat
     1621 acgttgattg tgttgtactt tgtgcgctat ttcgcgcagg gccggcgaca gcttcgggtc
     1681 tgtcgtcaat acggaaagag aagatttgga actcatcgca gacttgtgtt cggcagccgg
     1741 cacgcagccg cctcaatttt ggtacgaagc tataacagtc ggcgcaattt tcagtttttt
     1801 ttgaaagtaa aaataatgat tgctttgcgc gcaggggata tgaaggtgga atctcgcgcg
     1861 agcgaaaatc agcgcaatgc ctgcgtttca tgtagcttta cccaatccaa aatagcgctt
     1921 ttgattttat tttctccatt gtcgagttca tccacggaaa gtacgccacg aatagattgc
     1981 ttattccttg ccacccaccg gctacgcaac atgaccgcct catcttcatc tattttttcc
     2041 agtaagtaca aggcttcctc tacctcgctt ttgtccaggt ggagctttat ttccttgcaa
     2101 atttcagatc tggtgctata taattcactg cgaatgaata cgttgccgag cagggccgcc
     2161 acggagactt cgttcttttt gaacttgttg catcgcacgg tcgagttcct taaactcctc
     2221 caaatctttg agcgcataaa agtgcggctc ctccagcgtt ttgcaaccct cacagatgca
     2281 gggcaccatt ttcaccctgt caataccggg cggattgttc tgcggctcat ccaattcgcg
     2341 caggaggaag gacagcagga ttttgcgctt ttccaggtcc ttgccgcgca cccggatgtc
     2401 aatggttttt ttgttttcaa tttcgatcag gtaagcgagc gcctccatgt tttcccattc
     2461 caaccacacc tcgttttccc agacgtgatt gcggtttgct ccggcgatgt cgcggtgcat
     2521 gcccacaatg aagcgcgaaa acaaagcggg cggcaaaaag ccgtagcgca cttgcaggcg
     2581 caagtcatcg ggcgaagcgc cccagtccgc gtcgctgtct tgttttttgg gcagcagaga
     2641 tggggcgatg cggtcgtatc catgctcatc cttgtacttc aggtcgtagt agagcttgaa
     2701 gcgccgcatg agttcgttca attcgtcgaa atactcctcg aaatggtagc cctgatggat
     2761 atcttgccga ttaggctgcc agattctttc catgtcctgc tcgttgaaac gcccgaagcg
     2821 cgccttgacc tcttggtcgt ccagaatgcg atagacgcct tctacggacc agtcnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     2941 ggtactctaa gcagggttcc ggttcggcat ctatttcggg gcggcggcag atggcttcca
     3001 attccgtttt gggaatgatg ttgcgccctc tcatgccttc cagaatgccg cgcacctcct
     3061 gccagttggc cggcacggtg gagtggatgt gctccaattc ctgtaccgtc gtggccaact
     3121 tctcccggat gatgcccaac tctcgttgca gtcgttcttt gtcttgtgcg gaggcatttt
     3181 gcttttccgg ataccagcgg agcaggttgc agtcgtattt ctctttgaca agcgcctcca
     3241 ttttttgcac ccgttcgccc ctgaaatcgc ttttttcggt atcgtgcagg ttgttgatga
     3301 tgatgaccgg gctgccgccg ccgtacaggt tggctgtttc gcaccagaag ttccagtcgt
     3361 tggtatcgtc accgtttttg gtcactaaga tgtagagcgc cttgtggctg tagaaaaact
     3421 ggtgcagggg tttgtagtgg ttttgcccgc cgaagtcgaa gagattgaga cgaatttttt
     3481 caccgccggc cgtggacagc cacagcggtt ccacttctac ttccaagcct ttggtgcgct
     3541 cttccgaggg taccacgggc gcttccggcc catgcagcaa gcggcggcgc agggtggttt
     3601 tgccgcagcg gccgtcgccg atgagcaggg ctttggcttc cagacttttg acaaaacctt
     3661 tgcgggcgcc tgctttcagg aaggccagca cctgcgcctt gccgccggcc cacgtttccg
     3721 ggccgatttc ctccaagggg ttgccttcca attccaggtc ttccagacag ggcaaaccgc
     3781 cggcggggag ttcaaactgc ttgagttggc agtctcccag gtccaggatg cgcaactgcc
     3841 gcaaggccgg agcgggcgga aacacaaagg ctgtaagccc cccgttgtcg ctcaggttga
     3901 ggatgcgcag gtgctccagc gcgccgggga tgcggcagat gctttgccat tgggcatcgg
     3961 tcaggccggt tttgaccaag ttgagaccga tgaggcgctg ctgctcatcc actgcatact
     4021 tgggggtatt ttccttatga gggcgtacgc ctggcagctc gtcggagggg tactcgtcgg
     4081 cgggggccag gggtagccgg atgaacgcct cgatttgtag tactgcctcc ggcttggggt
     4141 aacgggaagt gaccgagggc gatagcggtt cgggttgatc ctgagagact attgcctcaa
     4201 agtgtgccaa cggagcctct tcgccttgcc tgctctcctg ctgtggctcg gagggtttat
     4261 ccccgaaaat ttttttcaaa aaatctttca tatcgctgcg gtgttgaggg tttcaaaata
     4321 ctccgatggc gcaagattcc atcttgtgcc gctattcggc gggttctacc cgccaccgtt
     4381 tgtttgattg taagcgaaaa atccgcaaca ggccacaaca ctctggcgcg agtggactaa
     4441 aaaaaagcgt tttgcgagcg ggtagaaccc gcataatact cgcacgagat ggaatctcgc
     4501 gcgagccgat actgaaaagt ttgaattgca tggctttttc gttttattcc aaaatgaaca
     4561 tgtttctctg cttcgtgcct gccggtcggg ctatcgcnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt tgcgaatggc cacggcgcag
     4801 cgcagcggcg cgctccagaa actgccgccg cgcaacacgc gcatcgagcc ttccttgcaa
     4861 aaaggattgt ctataggccc cgcacttttc gcgcaagttt tgtaaaaatc ctcatcatac
     4921 gcatccgcac accattccca tacattgccg ctcatgtcgt acaggcccaa ttggttgggg
     4981 tatttctgcc ccacctcgtg ggttttttgg ccgctgttgc cctcaa
//
LOCUS       DAORLF010000362         5021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9141_length_5021_cov_1.746476, whole genome shotgun sequence.
ACCESSION   DAORLF010000362 DAORLF010000000
VERSION     DAORLF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_5021_cov_1.746476"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>258)
                     /locus_tag="PK198_21430"
     CDS             complement(136..>258)
                     /locus_tag="PK198_21430"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRF41375.1"
                     /translation="PSAEHICFVIWNLLRRRLDERYDLQVRLYETPRNFVEYPA"
     assembly_gap    259..403
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<404..565)
                     /locus_tag="PK198_21435"
     CDS             complement(<404..565)
                     /locus_tag="PK198_21435"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRF41376.1"
                     /translation="MRVAAYRKAHFNAAHRLHNPDWSDEKNREVFGLCNNPNYHGHNY
                     ELEVRVAGEV"
     gene            714..1814
                     /locus_tag="PK198_21440"
     CDS             714..1814
                     /locus_tag="PK198_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRF41377.1"
                     /translation="MSKMFHSLRVKEVVKETSDTATLVFEIPQELTETFQYTQGQYLT
                     LRLSINGKEQRRSYSMCSSPLEKDIAVTVKRVKNGVVSNYLNDRVQAGNMLDVMEPEG
                     RFFTPLDPDNRKTYYLFGAGSGITPLFSILKTALEQEPQSFVHLLYGNRSEDSIIFKN
                     QLDALQRRYDGQFTVDYILSKPKRDKPGGLAGLFSKGTLSWQGKTGRINAVEVNKFLE
                     EHPLRNKNAEYFICGPGDLIDAVEKSLLEKGIDKKHIHAERFVSTHIAEADRIQGVGG
                     AEVAVQLNGKTVSINVPAGKTILDVLIDKKHNPPYSCTSGACSTCIAKVTEGSVKMDV
                     CYALDEDEVAQGFILTCQAHPTTPKVAVTFDV"
     gene            complement(1826..>2162)
                     /locus_tag="PK198_21445"
     CDS             complement(1826..>2162)
                     /locus_tag="PK198_21445"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRF41378.1"
                     /translation="LLQGKKATTNWYRAEEMLKKYGATFTNERFTQDGKYWTSAGVTA
                     GMDMSLAVMHDIWGKRYTQAVMLDMEYDPAPPISGGTPEKTGKVVYWMMKSMYDAGVQ
                     PLIDSLENR"
     assembly_gap    2163..2172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2173..2696)
                     /locus_tag="PK198_21450"
     CDS             complement(<2173..2696)
                     /locus_tag="PK198_21450"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRF41379.1"
                     /translation="MKILNILIAAVLLLAFSACKPKTEAVERTGITEENASEEVLPEG
                     VTEENHSMVMDLVLGKPKHQIRNIGILVYDGVNDLDMMGPKYVFGQSMGARTQLIAIQ
                     PGNIRTAMGTVIVPDNFIDSVSQLDILVIPGGARGTIETAYNDKVLLWIRQIDSTTQY
                     TTSVCTGAWVLGAAG"
     gene            complement(2696..3430)
                     /locus_tag="PK198_21455"
     CDS             complement(2696..3430)
                     /locus_tag="PK198_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41380.1"
                     /translation="MQKVKLHWFIRRTHRFMGVILGLQFLAWTLGGLYFSWTRIENIR
                     GENLRKEAALIPVSDSLAPLGEMLRSLSRTDSFGGISAVSLIEILGETHYQIQYQAGS
                     KKKVRLVHAGTGQWRSPLSEKEAVSLAQSRLLNPGPVESVAYLQATGSHHEYREKPLP
                     AYAVRFGAPSRTTVYVAAEWGTVQSFRNDQWRIFDFLWMLHTMDYQGRDDFNNALLRA
                     FSVFGLMTIFSGFALFFISQKHTKHS"
     gene            complement(3490..3951)
                     /locus_tag="PK198_21460"
     CDS             complement(3490..3951)
                     /locus_tag="PK198_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41381.1"
                     /translation="MKKLFFPLLILAAAVFGIWLYLRIQVWSNPEFFPAEATLIYLRD
                     SSMFLGMLRAVSLLFWGFGAWQLWSSGRAGYIWAAAVLYAGFLLLDGYLLVSQYYAYK
                     LTHEIWESGFPMSLFKGIPEAVAYLIFNAFVVWLLFDSRKKRARLEMQFYA"
     gene            complement(3963..4361)
                     /locus_tag="PK198_21465"
     CDS             complement(3963..4361)
                     /locus_tag="PK198_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41382.1"
                     /translation="MKKSLLLALLALATGMSFFACEKDKHDHEAEITILSPTAGQTFS
                     LNQTVSIQADITSEEELHGWEITLKKKSDNTVLYTTDEHTHGTSLQIREQWVNNVTAH
                     TDVVLEVKVALDHTSSETKTATVEFHCHPN"
     gene            complement(4376..>4684)
                     /locus_tag="PK198_21470"
     CDS             complement(4376..>4684)
                     /locus_tag="PK198_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF41383.1"
                     /translation="DDTGRHRITLNDEHRYQFKVPSLRNVEVTRPYMHDGRFRNLEAV
                     LDFYASGVADSPTLDPALKSGGRLGVELTATEKSNIIAFLHTLTDHEFLSKKELSEFH
                     "
     assembly_gap    4685..4694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4695..5001)
                     /locus_tag="PK198_21475"
     CDS             complement(<4695..5001)
                     /locus_tag="PK198_21475"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF41384.1"
                     /translation="MGEQMPNVLDKLRRHNKYPAMFTAAFGTPAITTDRFLRALSQFQ
                     LMCISSNSKYDKYVRNEGATLNAEELAGLQTFRQKCASCHEGELFTNNSYRNNGLPIA
                     "
BASE COUNT         1124 a         1286 c         1315 g         1131 t
ORIGIN      
        1 gccagctact ggctgcaagc cgcgcttagc accgcatacc gagaaccgcc caccgaactc
       61 tcgcgcttag caccgccgac cgcataccgc atactggtac ccccttcacc aagctggaga
      121 aaactgcccc gcaagtcagg ccgggtactc cacgaaattg cggggagttt cgtagaggcg
      181 cacctgaaga tcgtagcgct cgtctaagcg gcggcgcagc agattccaga tgacgaagca
      241 aatatgctcg gcgctgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacctcg ccggctacac
      421 gcacttccaa ttcgtaattg tgaccgtgat agttggggtt gttgcacagg ccgaagacct
      481 cgcggttttt ttcgtccgac cagtcggggt tgtgcaggcg gtgcgcggcg ttgaaatggg
      541 ctttgcgata agcggcaact ctcatgtttt tcttttttgc attgcaaaca gatgacccgc
      601 cggatggttg tagatgcacg gctaagggaa aagttatttg ccttgcgcaa tgtgggcgcc
      661 gtgccgtacc tttgccgccc gaaatcgcat cgcatcaacc acacactata tatatgagca
      721 agatgtttca ctcgctgcgc gtcaaagagg tcgtgaaaga aacctccgac actgctacgc
      781 tggtcttcga aatcccgcag gaactcacag aaaccttcca atatacgcag gggcaatatc
      841 tcacccttcg cttgagtatc aacggcaaag agcaacgccg ctcgtactcc atgtgcagca
      901 gccctttgga aaaagacatt gccgtgacgg tgaaaagagt gaaaaacggc gtcgtgtcca
      961 actacctcaa cgaccgcgtt caagccggta acatgctcga cgtgatggag ccggagggcc
     1021 gcttcttcac gccgctcgac cccgataatc gcaaaacgta ctatctcttc ggtgcgggca
     1081 gcggcatcac gcccttgttt tcgattctca aaacggcgct ggagcaggag ccgcagagtt
     1141 ttgttcacct gctgtatggc aaccgcagcg aagacagcat tattttcaaa aatcagttgg
     1201 atgcgctgca acgccgctac gacgggcagt tcaccgtgga ctacatcctc agcaagccca
     1261 aacgcgacaa acccggcggc ctggcaggtc tgttcagcaa gggcacgctt tcctggcagg
     1321 gcaaaaccgg ccgcatcaat gccgtggaag tgaataagtt tttggaggaa cacccgctgc
     1381 gcaacaaaaa cgccgagtac ttcatctgcg gccccggcga cctcattgac gcagtggaaa
     1441 aatcactctt ggaaaagggc attgacaaaa aacacataca cgccgagcgc ttcgtttcta
     1501 cccacattgc cgaggccgac cgcatacagg gcgtgggcgg agcagaggta gccgtgcaac
     1561 tcaacggcaa aaccgtcagc atcaacgtgc cggcgggcaa aaccatcctt gacgtgctga
     1621 ttgataaaaa acacaatccg ccgtactcct gcacttcggg cgcctgctct acctgcatcg
     1681 ccaaagtgac ggagggcagc gtaaaaatgg atgtgtgcta cgccttagac gaggacgagg
     1741 tggcacaggg ctttatcctc acctgccagg cgcatcctac tacgcccaaa gtggcggtta
     1801 cgtttgatgt gtgaggcttc tctcactaac ggttctctaa gctgtctatc agcggttgta
     1861 cgcctgcatc gtacatagac ttcatcatcc agtacacaac tttgcctgtt ttttcaggcg
     1921 ttccaccaga gatgggcggg gcggggtcgt attccatgtc caacatgacg gcctgggtgt
     1981 aacgcttgcc ccaaatatcg tgcattacgg ccagcgacat atccatgccg gccgtcacac
     2041 cggctgaagt ccaatatttg ccgtcttgag tgaatcgttc gttggtgaat gtagcgccat
     2101 atttcttcag catctcttcg gcgcgatacc agttggtggt agcttttttg ccttgcagca
     2161 atnnnnnnnn nncctgccgc tcccaaaacc caggcgccgg tgcataccga tgtggtgtat
     2221 tgagtggtgc tgtcaatttg ccttatccat agcaaaacct tgtcgttgta ggcggtttca
     2281 atggtacctc ttgctccgcc gggaatgacc aaaatgtcca attgggacac agaatcaatg
     2341 aagttgtcgg gtacaatgac ggtgcccata gcggtgcgga tattgccggg ctggatggcg
     2401 atgagctgcg tgcgggcgcc catgctttgg ccgaaaacat acttcggccc catcatatcc
     2461 aaatcattga caccgtcata aacgaggatg ccgatgttcc gtatttggtg tttgggtttt
     2521 cccaaaacca ggtccatcac catgctgtga ttttcttcgg tcacgccttc cggcaagact
     2581 tcctccgatg cattttcttc ggtaatgcct gtcctttcga cagcctccgt tttgggcttg
     2641 caggcactga aagcgagtaa aagaacagcg gcaatgagaa tgtttaatat tttcattatg
     2701 aatgttttgt atgtttttgg ctaataaaaa acaaggcaaa tcccgaaaaa atggtcatca
     2761 gcccgaacac ggaaaaagct ctcagcaggg cgttgttgaa atcatcccgc ccctgatagt
     2821 ccatcgtgtg caacatccac aaaaagtcga aaatgcgcca ttggtcgttg cgaaaactct
     2881 gcactgttcc ccactcggcg gccacatata cggtggtacg gctcggcgcg ccaaagcgaa
     2941 cggcataagc gggcagcggt ttttctcggt attcgtggtg gctgccggtg gcttgcaggt
     3001 aggcgacgga ttccacaggg ccggggttca ggaggcggct ttgagccaat gataccgctt
     3061 ccttttcgct caaagggctg cgccattggc cggtacccgc atgtacgagg cgaacctttt
     3121 ttttgctacc tgcctgatat tgaatctggt aatgcgtttc tcccaaaatt tctatcaggg
     3181 agacggcaga aatgcctccg aagctgtctg tacggctcaa actccggagc atttcgccca
     3241 aaggcgccag gctgtcggaa accgggatga gggccgcttc tttccgaagg ttttcgccgc
     3301 gaatgttttc aatacgggtc caactgaaat acagcccgcc gagcgtccag gccaaaaatt
     3361 gcagtcccag tatgacgccc ataaaacggt gcgtacgtcg tatgaaccaa tgtagtttta
     3421 ctttttgcat aggatgagag gtgtaaaaaa gggagccgac ccattggccg gccccttcaa
     3481 atataactat catgcataaa attgcatctc cagacgcgcc cgcttcttcc gactgtcgaa
     3541 caacagccag acaacgaacg cattaaaaat caaataagca accgcctccg gtattccttt
     3601 aaaaaggctc atcggaaacc cgctttccca gatttcatgg gtcagtttgt acgcgtaata
     3661 ctgcgacacg agcaaataac cgtccaacag caggaagccg gcatacaata cggccgcagc
     3721 ccagatatac ccagcccggc ccgatgacca gagctgccag gcgccaaagc cccagaacag
     3781 aagcgacaca gcccgcaaca tgccgagaaa catggagctg tcgcgcaaat agatcagagt
     3841 ggcctctgcc ggaaaaaact cagggttgct ccacacctga atacgcaggt acagccagat
     3901 gccgaaaacg gcggcggcca atatcagcag cgggaaaaac agctttttca tggcggtgca
     3961 ttttagttgg gatggcaatg gaattcaact gtggcggtct tggtttcgct gcttgtgtga
     4021 tccaaggcca ctttcacctc cagcactacg tctgtgtggg cagttacgtt gttcacccat
     4081 tgttcgcgga tttggagaga ggtgccgtgc gtatgctcgt cggtggtgta cagtaccgtg
     4141 ttgtcggatt tctttttcaa agtaatttcc cagccgtgca attcctcttc ggaagtaata
     4201 tcggcctgaa tggacacggt ctgattgagc gagaaggttt ggccggcggt gggcgagagg
     4261 atggtgattt ctgcctcgtg gtcgtgcttg tctttttcgc aggcaaagaa agacatgccg
     4321 gtagccagcg cgaggagtgc gagaagaagt gattttttca tggttgttgt tgattttaat
     4381 gaaattcaga aagttctttt ttgctcagga attcgtggtc ggtcagggta tgcaggaagg
     4441 cgatgatgtt gcttttttct gtggccgtga gttctacacc gagccgcccg cccgacttca
     4501 gggccggatc gagggtgggg ctgtcggcaa ccccggatgc gtaaaaatcg agtacggctt
     4561 ccagattgcg gaagcgcccg tcgtgcatgt atgggcgggt cacttccaca ttgcgcaggc
     4621 tgggcacctt gaattgatag cggtgctcgt cgttcagcgt gatgcggtgc cgtccggtgt
     4681 cgtcnnnnnn nnnntggcaa tgggcagccc gttgttgcgg tagctgttgt tggtaaacaa
     4741 ttcgccttcg tggcaggaag cgcatttttg gcgaaaggtt tgcaggccgg ctaattcttc
     4801 ggcgttgaga gtagcgccct cgttgcggac gtacttgtcg tatttcgagt tggaggagat
     4861 gcacatcaac tgaaactgcg ataaggctct gaggaagcga tctgtggtaa ttgcgggcgt
     4921 gccgaaagcc gccgtaaaca tggcggggta cttgttgtgg cggcgcaatt tgtccagcac
     4981 attgggcatc tgctctccca tttcattctc attttcaatg g
//
LOCUS       DAORLF010000363         5019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9147_length_5019_cov_1.912369, whole genome shotgun sequence.
ACCESSION   DAORLF010000363 DAORLF010000000
VERSION     DAORLF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9147_length_5019_cov_1.912369"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PK198_21480"
     CDS             <1..1279
                     /locus_tag="PK198_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41385.1"
                     /translation="LALDRLVKEFPNSEFADDALLAMGSTYQEMGQLNQAVAPLRQLV
                     TNYKGKSDLINQALLRLGLISYNQANLEGAINYYKQVFSNNPDPNEAALALAALEEIY
                     VGDMGKPDAYFAFLETIPGYKVDNIAKDSISYKAALTQFENGNYPRAVDGYTDYLRKY
                     PNGIYVIDAYFQRGESYAVQKQYSAALKDYEFVVGRGQNRHYLKALDKASIIAYNHEL
                     DFNKSYDLYTKLEAAATSAEMRFEAQLGALRSAYRIGNAQAVATYANKVSTNPAATPA
                     QAATANFYIGKQAYDKKDFNAALASLQEVVRLSDNEQTAEARYLIADIYYQRRDLNKA
                     QELCISANRESSAYPYWVAKTVILLSDVFLEKGELQNARAVLEAVLENYNEDPDLVKT
                     PQTKLAAVNARINSGSRLDVNKNTNRLEMDTRN"
     gene            1339..2367
                     /locus_tag="PK198_21485"
     CDS             1339..2367
                     /locus_tag="PK198_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41386.1"
                     /translation="MSKYFALIAVALISASVALQAQTTLPTEQVEIVKNFDARLLETE
                     RIGLQPELPPLDTSVKRQTYNITGRPINVEYPAPQIRPLAMRGERVPKVYNGYTRLGA
                     GMPSSLLGEFSYNIAKDKKYNFGIGAQHHSANNNAKVENQRFSYTSAGLDGTYYLEQG
                     IAINGKARYTADNVFFYGYNNLNDPDNPAISFDKEDVRQRFSIIDVGANIFNGERTVA
                     DFNYQAGFDFYYLQDAYAARENGFDLKINATKWIQERHAFRINLRTDFTSYRDTARQS
                     LHNFYLQPNFTFSGDAFRAKVGLNLVSHDDEFSFFPDVELSARVIGPVLNVLRAPRVL
                     CKKTPSAV"
     gene            2340..2972
                     /locus_tag="PK198_21490"
     CDS             2340..2972
                     /locus_tag="PK198_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41387.1"
                     /translation="MQKNTFRSLSEYNPFVSSRIRLRNTRYIDYFGGIRGVVQGIDYR
                     VQGGYKTTDHLATFLWNGDSLPPRFNVLYDTIDIAYITASLVAPLFKGFEINGKFTQN
                     FFNAGKEGKAWHLPATTISAGAKYATTDGKGSVRADLFLENGVPYLDTDGVAKNLNAL
                     FDLSLGADYRLTENIGVFLQLNNLANNRRQRWQHYPVFGLNALAGITARF"
     gene            3151..3417
                     /locus_tag="PK198_21495"
     CDS             3151..3417
                     /locus_tag="PK198_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRF41388.1"
                     /translation="MYITFDELRDIKHKLPTGSVKLIADKLNIDEQTVRNYFGASKYE
                     DDQSIPGIHIQPGPNGGIVHIEDTSILDLAKSLIPETEGEAAEN"
     gene            3422..>3669
                     /locus_tag="PK198_21500"
     CDS             3422..>3669
                     /locus_tag="PK198_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HRF41389.1"
                     /translation="METADPPLSGLDFLAGEVLLINKPKGWTSFDVVNKLRNKLRQLL
                     QVKNIKVGHAGTLDPMATGLLIVCTGKATRRIDEYQNLP"
     assembly_gap    3670..3679
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3680..4135
                     /locus_tag="PK198_21505"
     CDS             <3680..4135
                     /locus_tag="PK198_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HRF41390.1"
                     /translation="KVYTGTMMMGAVTASYDAETEVEETFPIQHITPELLEQARLTFL
                     GDLEQIPPMFSAIKVDGQPLYKKARKGEHIAIAARPVHISRFDLSRVELPEIDFEVHC
                     SKGTYIRSLAYDFGKALQSGAYLSALCRTAIGPHLLKDAWELEELIQRL"
     gene            4354..4857
                     /locus_tag="PK198_21510"
     CDS             4354..4857
                     /locus_tag="PK198_21510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41391.1"
                     /translation="MKHFLILVFCGMAVGAAAQAAIERQVTASQGGSVSNNRLSLDWT
                     LGEPAIATLYTSFGQLTQGFQQPSIWVETVQPSPALVRTAADIRISPNPTAAEINIFF
                     PADLSGEVWLEISDLQGKPLLREKTDASAATWHIDASAYPPGSYLLRCISARGEVLSI
                     HRIVKTK"
BASE COUNT         1301 a         1387 c         1148 g         1173 t
ORIGIN      
        1 cctggcattg gatcgcttag taaaggagtt tccgaactct gaatttgccg acgatgccct
       61 actggcaatg ggttctacct atcaggaaat gggacagctc aatcaggcgg tggcgccgct
      121 gcgccagttg gtaaccaatt acaaaggcaa atccgatctc atcaatcagg cattgctgcg
      181 tctcggtctc atcagctaca accaggccaa cctcgaaggc gctatcaact attacaagca
      241 agtgttttcc aacaatcccg accccaacga ggcagccctc gcgctggcag ctttggaaga
      301 gatttatgtg ggagatatgg gcaagcccga tgcttatttt gcatttttgg aaaccattcc
      361 gggttacaaa gtggacaaca ttgccaagga ttccatcagc tacaaagccg cgctcacgca
      421 gtttgaaaac ggcaactacc cccgcgctgt ggatggctac accgactatc tgcgcaaata
      481 ccccaatggc atttatgtga ttgatgctta cttccagcgc ggcgaatcgt atgctgtgca
      541 aaagcaatat tcggctgcac tgaaggatta tgagtttgta gtaggacgcg gccaaaaccg
      601 ccactacctc aaggcactcg acaaggcatc catcatcgcc tacaaccatg aattggattt
      661 caataagagc tacgatcttt acaccaaatt ggaagcggcg gccaccagcg cggagatgcg
      721 ttttgaagcg caactcggcg ccctgagaag cgcctatcgt atcggcaatg cacaggcagt
      781 ggcaacgtat gccaacaaag tgtccaccaa tcccgcagct acgcccgcac aggcagctac
      841 ggccaatttt tacatcggca aacaagctta cgacaaaaaa gacttcaacg ccgctctggc
      901 atcccttcag gaggtggtgc gcctgagcga caacgagcaa accgccgaag cccgctatct
      961 catcgccgat atttattacc agcgccgcga cctcaataaa gcacaggagc tttgtatcag
     1021 cgccaatcgc gaaagctcgg cttatcctta ttgggtggcc aaaacggtga ttttgttgtc
     1081 ggatgtattc ctcgaaaaag gagaattgca aaatgcccgc gctgtgttgg aggccgtttt
     1141 ggaaaactac aacgaagacc cggatttggt caaaacgcca caaaccaaat tggccgctgt
     1201 gaatgcccgg atcaactccg gcagccgttt ggatgtaaat aaaaacacca atcgcttaga
     1261 gatggacacc cgaaattgag ttcgtccaac gcggctcttt tgtccaattg attgaaaatc
     1321 ataaaaatgc aacaaacaat gtctaaatat tttgctttga tcgcagtagc gcttatctct
     1381 gcttcggtcg cacttcaggc acaaaccacc ctgccgacgg agcaggtgga aattgtaaaa
     1441 aacttcgatg cccgcctgct cgaaaccgag cgcatcggcc tgcaacccga actgccgcct
     1501 ttggatacct ctgtaaaaag gcagacctac aacatcaccg gccgcccgat caacgtagag
     1561 tatccggcgc cgcagatccg ccctttggcc atgcgcggcg aacgcgtacc gaaagtatat
     1621 aacggctata ccagactggg tgcgggcatg ccgtcttccc tgctgggtga gttttcttac
     1681 aatattgcca aagacaaaaa gtacaatttc ggcatcggcg cacaacacca ctcggccaac
     1741 aacaatgcca aagtggaaaa ccagcgcttt tcttacacca gcgccggttt agacggaacg
     1801 tactacctgg agcagggcat cgccatcaac ggcaaggcgc gctacactgc cgacaacgtc
     1861 ttcttctatg gctataataa cctcaacgat cccgacaatc cggccatcag ttttgataaa
     1921 gaagacgttc gacagcgttt ttctataatt gatgtaggcg ccaatatctt caacggcgag
     1981 cgtaccgttg cagatttcaa ctatcaggcc ggattcgatt tctattacct gcaagatgct
     2041 tatgccgccc gcgagaatgg tttcgacctc aaaatcaatg ctaccaagtg gatacaggaa
     2101 cgccatgctt tccgcatcaa tctgcgcacc gattttactt cgtaccgcga cacagcccgg
     2161 caatcgctgc acaacttcta cctacagccc aatttcacct tttcaggaga tgctttccgc
     2221 gccaaagtgg gcttgaatct cgtttcgcac gacgacgagt tttcgttctt ccccgatgtt
     2281 gaattatcgg ccagagtcat cgggccggta ctcaatgttt tgcgggcgcc gagggttctt
     2341 tgcaaaaaaa caccttccgc agtctgagcg aatacaatcc gtttgtcagt tcccgcattc
     2401 gcctgcgcaa cacgcgctac atagactact tcggcggtat tcgcggcgta gtgcagggca
     2461 ttgactaccg ggtgcagggc ggttataaaa ccaccgacca tttggccaca ttcctgtgga
     2521 acggcgacag tttgccgccc cgtttcaacg tattgtacga caccatagac attgcataca
     2581 tcactgcatc cttggtggcg ccgctgttca aaggttttga aatcaacggc aaattcaccc
     2641 aaaatttctt caatgcaggc aaagaaggca aagcgtggca cttgcccgcc actaccatct
     2701 cggcaggcgc caaatacgct accaccgatg gtaaaggctc ggtgcgtgcc gatcttttct
     2761 tggaaaacgg cgtgccctat ttggatacgg acggagtagc aaaaaatctc aacgcgctgt
     2821 ttgacctgag cttgggggcc gattatcgcc tgacggaaaa catcggtgtt tttctgcaac
     2881 tcaacaactt agccaacaac cggcgtcagc gctggcagca ttatccagtg ttcggcctca
     2941 atgcactggc cggcattacg gcgagattct gattactaca aaagccttga gcttcgacat
     3001 gcaacccgga ctccgaccct gcgtcagagt ccgggttgtc gtttttttag cacctgtgtt
     3061 aattttttta agcaaacgct tgcatacaat cgtttcccgc cttacctttc cgaaaattga
     3121 aatcacattg ttcacaaaac attgctaatc atgtatatca cttttgacga actgcgcgac
     3181 atcaaacaca agctgcctac cggcagtgta aaactcattg ccgacaagct caacatagat
     3241 gagcaaaccg tgcgcaacta cttcggcgca tccaaatacg aagacgacca gtccattcca
     3301 ggtattcaca ttcaacccgg ccccaatggc ggcatcgttc acatcgagga tacctccatt
     3361 cttgatttag ccaaatcttt gattccggaa acagaaggcg aggctgccga gaattaaact
     3421 tatggaaaca gcagaccctc ctctttccgg gcttgatttc ctggccggcg aagtacttct
     3481 catcaacaaa ccgaagggct ggacttcgtt cgatgtggtc aacaagttac ggaacaagct
     3541 gcggcaactg ttgcaggtaa aaaacattaa ggttggccat gcgggtacat tggaccccat
     3601 ggccaccggc ctgctcattg tatgcaccgg caaagccacc cgccgaattg acgaatatca
     3661 aaacctcccn nnnnnnnnna aagtttatac cggcactatg atgatgggcg ccgtcactgc
     3721 ttcttacgat gcagaaactg aggtggaaga gacattcccc atacaacaca tcacaccgga
     3781 attgctggag caggcccgat tgacgtttct gggcgactta gagcaaattc cccccatgtt
     3841 ctctgctatc aaagtggacg ggcagcccct gtacaaaaaa gcgcgcaagg gtgaacacat
     3901 tgccattgcc gcacgccctg tccatatttc cagatttgac ctcagccgtg tggaactccc
     3961 cgaaattgac ttcgaggtgc attgttccaa gggtacttac atccgctctt tagcttatga
     4021 ttttggcaaa gcgctgcaaa gcggcgccta tctcagcgca ctgtgtcgca cggccattgg
     4081 tccacacctg ctgaaggacg cctgggagct ggaagagttg attcaaaggc tataactcca
     4141 aaatcgcggt caaaggaacg ggttccaaaa ataaaaaaac ggccataaaa ttttattttt
     4201 ttagctcaaa gccctatact tgcgctatca tttccgcatt tgccctattc tgcatggaaa
     4261 atgattgccg ggcgctccgc ttccccccct tgttgcgctc atttgtgaca gccacgctat
     4321 catatagatt gttcacctta caaactcata ttcatgaagc atttcctgat tctggtattt
     4381 tgtggcatgg cagtcggcgc agcggcgcaa gcagccatcg aacggcaagt gacagcttcg
     4441 caaggcggtt ccgtgtcaaa caatcgtctc agtttagact ggacgctggg cgaacctgcc
     4501 attgcaacac tctacacctc ctttgggcaa ttgacccagg gatttcaaca gccctctatt
     4561 tgggtagaaa cagttcagcc atcgcccgct cttgttcgga cggcagccga cattcgcata
     4621 tctcctaatc ccaccgctgc ggagatcaat attttttttc ctgctgatct ttctggagaa
     4681 gtatggctcg aaatcagcga cctacaaggc aaacccttgt tgcgcgaaaa aaccgatgcc
     4741 tccgccgcaa cctggcatat agacgcgtcg gcatatccgc cgggttctta tttgttgcgg
     4801 tgtataagcg ctcgcggaga ggttctctcc attcacagga ttgtaaaaac gaaatagccc
     4861 cgactcaatt gatgaatact ttcacttcct aaaaactcac acaattatga aaaagcacct
     4921 tattctggcc attgcttttt gcctggcagg tcttttcgcc tttgcccaga gcataccgca
     4981 gggcatcaat tatcaggccg tagcacgcga ctcgaaagg
//
LOCUS       DAORLF010000364         4999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9187_length_4999_cov_1.941343, whole genome shotgun sequence.
ACCESSION   DAORLF010000364 DAORLF010000000
VERSION     DAORLF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_4999_cov_1.941343"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PK198_21515"
     CDS             complement(<1..1482)
                     /locus_tag="PK198_21515"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF41392.1"
                     /translation="MRLLATDVPQEQLLKTVSYKTNFADSSGVFSELSAVLRQLHDQS
                     MLEASVDTVARSDSIFTAFIHVGSGFSWLELLPGNAQPDQLKAAGFRASDYRHRPLQW
                     SRWKKLQINLLQQYQNKGHPFAQVSLGSLAFEQEKIAAAIDVQPGRSFQFGKLELEGN
                     LRISPTYLYRYLGIHPGEPYHHGRVLQVRDRLRALPFAALKQDPQILFRQDSTADVLL
                     HLDSRKAGRFDFLIGVLPNNSQTGRMLITGSLFADFYNQFGKGERITASFERLRPETQ
                     QVAFLFKYPYLLQLPFGVDARFQLYKRDTTFLNLEYSAGADYLLEGASYWKAFWQHRS
                     TRLTGIDESRLLTTRQLPAQLDVSNDAFGLETSQQKTDYRLNPRRGWTGMLRASAGVK
                     RVKPNTRIVALGLAELYDTIAANSAQYRLEFSGEYFVPVWSSAAVKWGLQGGWLLSKT
                     PVFQNEQFRIGGNRLLRGFDEEFFFATGYAVATAELRLLIGQNS"
     gene            complement(1591..>3204)
                     /locus_tag="PK198_21520"
     CDS             complement(1591..>3204)
                     /locus_tag="PK198_21520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41393.1"
                     /translation="KGMVYWYLSDYQEAANLFFQSLGIREKLKDTLGIARSYNNIGNA
                     YFLQKNYKEAERYYQQSLDWRQTLRDTAGLIYSYNNLADVHLSRNNLDAAELLYQSAL
                     QIAGGNLDGQAFIHRNLGLLRIRQGRQEEALQHFQTSKELSEQTNNINNLALALIQIA
                     QVHFPNKDYQLVIKNALTAFEISRPSGLEASASEAAQLLAQAYAGLGKFEDAFRYQLI
                     YNEIQGNIWEKQSQNSIIEIQAKHELADLRSESERALLQKQKQISRLVILSLACVFIV
                     VLSFLFFQIYRYRTQMKTAILLEEKNEAIQKQNEALLKSNIALERYAFVASHDLKEPL
                     RTVGSFASLLKRRYKNQLDEDGLSFIQNISNGVDQMSMLLEDLLTLAKAGSESEYQKS
                     LINLNELVDDVLNNLNARITEQNARIEKTDMPNVYMNPSHGLQLFQNLLSNALKFSDK
                     EQCVIRIGCEKKAAHFLFSVSDNGMGIPPEWQDKVFLPFHRLAHKGKSGAGLGLAICD
                     KIVRLYGGTIWVESEPGDGSTFYFTLPVG"
     assembly_gap    3205..3362
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3517..3864)
                     /locus_tag="PK198_21525"
     CDS             complement(3517..3864)
                     /locus_tag="PK198_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF41394.1"
                     /translation="MAIMITDECINCGACEPECPNNAIYEGGLEWAITDGTSIKGTYT
                     LEDGTQVDAQQKQKPVSDDLYYIVPDKCTECVGFHEEPQCAAVCPVDCCVPDPERAED
                     EAILLDRKARLHL"
     gene            complement(3938..4939)
                     /locus_tag="PK198_21530"
     CDS             complement(3938..4939)
                     /locus_tag="PK198_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF41395.1"
                     /translation="MKAIIPVAGTGAHLRPLTYTQPKPLIPVAGKPIVSYMIDKLLEV
                     GVRDFVFIIGYLGEKIKEFIESAYPDINKEFVQQDERLGSGHAIWKAKDKFRDADEVI
                     IFFGDVIIETDYHKFIQLPGSAIGVKKVLDPREFGIVELDEQGMVQRVTEKPRIPISD
                     LAMVGLYKIAQVQTFVDALDYLVFNNIRDKSEFPLTDALMRMIQQENTFTPVVVDRWF
                     DCGKKEVLLETNALLLRREEYDPQSVPTFDNSIIIHPVSIGRHCDISNSIIGPHATIG
                     DNARINNAIVRDSIIGNFASLSEVILRHSVIGNDTSIVGLKHSLNIGDNTEIDFSEG"
BASE COUNT         1173 a         1162 c         1252 g         1254 t
ORIGIN      
        1 cgaattttgg ccgatcagca gcctcagttc ggcggtagct acggcgtagc cggtggcaaa
       61 aaagaactcc tcatcaaaac cccgcaagag ccggttgccg ccgatgcgaa actgctcgtt
      121 ttgaaacaca ggcgttttcg acaacaacca gccgccctgc aagccccatt tgacggcggc
      181 actgctccat accggcacaa aatattcgcc gctgaattcc agccggtact gagcgctgtt
      241 ggcggcaatg gtgtcgtaca gttcggccag ccccagtgcc acgatgcgcg tattgggttt
      301 cacccgtttt actccggcag aggcccgcag catacccgtc cagccgcgcc ggggattcag
      361 gcggtagtcg gttttttgct gagaggtttc caatccgaag gcatcgttgg aaacgtccaa
      421 ttgggcgggc aactgcctgg tagtcaataa tctggattcg tcaattccgg taaggcgggt
      481 gctgcggtgt tgccagaatg ctttccaata agaggcgcct tccagcaaat aatcggcgcc
      541 ggcgctgtac tctaaattga gaaaggtggt atcccgtttg taaagttgaa aacgtgcatc
      601 cacaccgaag ggcaattgca aaagataggg gtatttgaat aaaaaagcga cttgctgggt
      661 ctccgggcgc agtcgttcaa aggatgccgt gatgcgctcg ccttttccga attggttgta
      721 aaaatcggca aaaaggctgc ccgtgatgag catgcggcct gtctgcgaat tattgggcaa
      781 cacgccgatg agaaaatcga aacggccggc tttgcgggaa tcaaggtgca ggagcacatc
      841 ggcggtgctg tcctgccgga agagaatttg ggggtcttgt ttcagcgcgg cgaagggcaa
      901 tgcgcgcagc cggtcgcgca cttgcagcac cctgccatga tggtatggct cgccgggatg
      961 gatgcccaaa taccggtaaa gataggtggg agagatgcgc aaattgcctt ctaattccag
     1021 ttttccaaat tgaaaagagc ggcccggttg cacgtcaatg gcggcggcga ttttttcttg
     1081 ctcaaatgcg aggctgccca gactcacttg ggcaaacggg tggcctttgt tttggtattg
     1141 ttgcaacaaa ttgatttgca gctttttcca gcgcgaccat tgcagcgggc gatggcgata
     1201 atcggaggcg cgaaatccgg cagccttgag ttgatccggt tgtgcattgc cgggtaagag
     1261 ttccaaccac gaaaacccgg aacccacatg aatgaaagcc gtaaaaatgc tgtcgctgcg
     1321 ggccaccgta tccaccgatg cctccaacat ggactggtcg tgcaattgac gaaggacggc
     1381 gctcagttca gaaaacacgc ccgacgaatc ggcgaaattg gttttgtacg aaacggtctt
     1441 taataattgt tcctgcggta catccgtagc cagcaggcgc aatgtatagc ttttttgtgc
     1501 tgttgagcgg cagattgtca acaaggcgaa agacagtacc agaacgtata ttcgattcac
     1561 ttcggaataa cggctttatt ggggtgggca ttacccgaca gggagcgtaa agtaaaaggt
     1621 gctgccatct cccggttctg attccaccca tatcgtacct ccataaaggc gcacaatttt
     1681 gtcgcatata gccagcccca gccccgctcc cgattttcct ttgtgcgcca gccgatggaa
     1741 gggcaggaag accttgtctt gccattcggg aggaataccc atgccgttgt cggaaaccga
     1801 aaaaagaaaa tgcgctgcct ttttttcaca accgatacga atgacgcatt gctctttgtc
     1861 gctgaatttg agggcattgc tgagcaggtt ttgaaaaagc tgaagcccgt gcgaggggtt
     1921 catataaacg ttgggcatat ccgttttttc aatccgggca ttctgttctg ttatgcgggc
     1981 attcagattg ttcaaaacat cgtcaaccaa ttcattgaga ttgatcaatg atttttgata
     2041 ctcagactcg ctgcctgctt tcgccaaagt aagcaggtct tccaacaaca tggacatctg
     2101 gtcaacgccg ttcgatatgt tttgtataaa agaaaggccg tcttcgtcca actgattttt
     2161 ataccgcctc ttcaataaag aagcaaaaga ccccaccgtg cgcaatggct ctttgagatc
     2221 gtgagaggct acgaatgcat accgttccaa ggcaatattg gacttcagca gcgcttcgtt
     2281 ttgcttttgt atggcttcat tcttttcttc cagcaaaatc gccgtcttca tttgggttcg
     2341 gtagcggtat atctggaaaa acaaaaaact aagcaccact ataaatacgc aagcgaggga
     2401 aaggataaca agcctgctga tttgcttttg tttttgcagc agcgctcttt cagactcaga
     2461 ccggagatcg gcaagttcgt gtttggcttg tatctcaatg atggaatttt gggattgttt
     2521 ttcccatata ttcccttgta tttcattgta aatcaactga taccgaaagg cgtcctcaaa
     2581 ttttcccaac ccggcataag cctgtgccag caattgcgca gcctccgagg ccgaggcttc
     2641 taatccggag gggcggctga tttcaaaagc tgtcagggca ttcttgataa ccagttggta
     2701 atctttgttt gggaaatgca cttgcgcaat ttggatcagc gccaaagcca ggttgttgat
     2761 gttattcgtt tgttctgaca gttctttaga ggtctgaaaa tgctgcaaag cttcctcctg
     2821 ccgcccttgc ctgatcctga gcaaccccag attgcgatgg ataaaagcct gaccgtccaa
     2881 gttgcctcct gctatttgga gagcggattg atacaatagt tcggcagcgt caaggttgtt
     2941 tcggctcaga tgtacatcgg ccaggttgtt gtatgagtat atcaaacccg ccgtgtcgcg
     3001 cagggtttgt ctccaatcta aactttgctg gtagtatctt tcggcttcct tataattttt
     3061 ctgaaggaaa tacgcattgc ctatgttgtt gtaagaacgg gcaataccca gtgtgtcttt
     3121 taatttttct ctgattccca gagattgaaa aaacaagttt gcggcctcct gataatcgct
     3181 gagataccaa taaaccatgc ccttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnaggttgct ttgctgtaac aaagcttgca gagaatcccg caccgactga gcatccgccc
     3421 ggcataaaaa gagcatcata caaagtatgc tgatgatgcg gatggatact ttgttgatac
     3481 tcacgaagcg ttatctgaac taaaaatttg aacgccctac aagtgcaatc gcgccttgcg
     3541 atccaacaaa atggcttcgt cttccgctct ttccggatcg ggtacgcagc agtccacagg
     3601 gcaaacggct gcgcactgcg gctcttcgtg aaagcctaca cactcggtgc atttatcggg
     3661 cacgatgtaa tacaaatcgt cggaaacggg cttttgcttt tgctgagcat ctacttgcgt
     3721 gccgtcttcc agcgtatatg ttcctttgat ggaagtaccg tctgtgatgg cccactccaa
     3781 tccgccctca taaatggcat tgttggggca ttcgggttcg caggcgccgc agttgatgca
     3841 ctcgtcggta atcattattg ccatgacaat ctcattttat agggaaataa cactgatttc
     3901 ccggcttggt ttgaggccgc aaaggtagga aaaaagatca accctcggaa aaatcaattt
     3961 cggtattgtc gccgatgttg agactgtgtt tgaggcccac aatagaggta tcgttgccaa
     4021 tgacggaatg ccgcagaatg acttcgctga gggaagcaaa attgccgatg atggaatctc
     4081 tgacaatggc gttgttgatg cgggcattgt cgccgatggt ggcgtggggg ccgatgatgg
     4141 agttgctgat atcgcagtgc cgcccgatgc tgacggggtg aatgatgatg gaattgtcaa
     4201 aagtaggcac cgactgtgga tcgtattctt ctcggcgcag gagcagggca ttggtttcga
     4261 gcaggacctc ctttttgcca caatcgaacc aacgatccac tacaacgggg gtgaaagtat
     4321 tttcctgctg aatcatacgc atgagcgcat cggtgagcgg aaactcgctt ttgtctctga
     4381 tgttgttgaa aaccagatag tctaaggcat ctacaaaagt ctgtacctga gcaattttgt
     4441 aaagacctac catggccaga tcggatatgg ggatgcgggg cttttccgtc acccgttgca
     4501 ccatgccctg ttcgtccagt tcaacgatgc cgaattcgcg gggatcgagt acttttttaa
     4561 cgccgatggc cgatccgggc agttggatga acttgtggta gtcggtttca ataatgacgt
     4621 ctccaaaaaa aataatgacc tcatcggcat cccgaaactt atccttggcc ttccagatgg
     4681 catggcccga gccgagccgc tcgtcttgct gcacaaattc tttgttgatg tcgggataag
     4741 cagactctat aaattctttt attttctctc ccagatagcc gatgataaag acaaaatcgc
     4801 gcacgcctac ctccaacagt ttgtctatca tataggagac aatgggcttg cccgccactg
     4861 gtatcaacgg cttgggttgc gtataagtga gcggacgaag gtgtgctccg gtaccggcta
     4921 cgggtatgat tgctttcatg gcgaaacgac gctgctactt agtagtgggg caaaaataaa
     4981 gtgttcattt tctttaggc
//
LOCUS       DAORLF010000365         4982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9238_length_4982_cov_2.014410, whole genome shotgun sequence.
ACCESSION   DAORLF010000365 DAORLF010000000
VERSION     DAORLF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9238_length_4982_cov_2.014410"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>323)
                     /locus_tag="PK198_21535"
     CDS             complement(<1..>323)
                     /locus_tag="PK198_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41396.1"
                     /translation="PPGLTDGVHALAISPQVDNARYNCSVTGPTVAVLRAALYQQSNW
                     LTNDNIRFVLPPPCNFLCGLGTDNRPPLVFCPFSMEGFVDETCDYTLKDFRNAVFVSD
                     NCGSY"
     assembly_gap    324..351
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<352..846)
                     /locus_tag="PK198_21540"
     CDS             complement(<352..846)
                     /locus_tag="PK198_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41397.1"
                     /translation="MRTYQHTAAARIIPAALCLLLFWQIGATQTTLSAGDIAFTGYNS
                     DNPDDFSFVLLKDVSAGTTLTFTDKGWTAAGGFRPGESTMTITFSSARVVGEQFRVPA
                     IMGVFLDFDGLSAGSSSGANILLSASGDQIFAYQGSEPFNNSPAEQAKFIAALHMNGL
                     WQTDD"
     gene            1084..1716
                     /locus_tag="PK198_21545"
     CDS             1084..1716
                     /locus_tag="PK198_21545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF41398.1"
                     /translation="MKDNFCVYLDAGHGAIDPKGNYTTAPSKMFDHKTGTFHKGSVFY
                     EGVWNRALVNRVSAKLQTLGIRHLMVHHEYLDTPLQQRVDTANWYHKNFAEGIYISTH
                     SNAANSQARGFEVYTSKGQTASDPIATMMYNHVANLLGNRITMRPDNSDGDPDREAAF
                     FVLTKTAMPAILIEHLFFDNLEDARLLMNDDIVDLFAEAQVRTIVEHFSK"
     gene            1726..2373
                     /locus_tag="PK198_21550"
     CDS             1726..2373
                     /locus_tag="PK198_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRF41399.1"
                     /translation="MKDTFLTLAAPALGEFKDRGSKFIAYAFPIESESDWHQRLEEVR
                     KEHPKARHYCFAFRLGQDGLLYRANDDGEPAGSAGRPILGQIDSFGLTNVFVVVVRYF
                     GGTLLGVPGLIAAYKESTQDALQQAEIVEKIIVQTFRVQYDYGLTSQIMNAVKKLSFE
                     LAEQEFGDTGATLEMRIRKSEADQKILEFKALAGNLHLEEAAVVEEIPGLMIEWV"
     gene            complement(2452..3891)
                     /locus_tag="PK198_21555"
     CDS             complement(2452..3891)
                     /locus_tag="PK198_21555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRF41400.1"
                     /translation="MKTTLKFLLLAVVLHSAVSCAVNPVTGKKQLILMSEEQEKALGI
                     QSDPEIIAGMGLYEDAGLQQFINEKGKQMAAVSHRPNLGYEFKIVDSPVVNAFAVPGG
                     FVYFTRGIMAHFNNEAEFAGVLGHEIGHVTARHSAQQYSNQMLGQVGLMAGVIISEDF
                     RKIAGAAQTGMGLLFLKFGRDHETQSDGLGVEYSSKIGYDANEMAGFFKTLQRLSDQG
                     GGRVPTFLSTHPDPGDRNVKVAADAAKWQQARPGQYAVGRDSYLRMIDNIIYGEDPRQ
                     GYVEGGVFYHPVLKFQFPVPADWQHENSPQQFQMAPKDGKALMVMSLSQAKTLQEAAQ
                     GLAQEAQLTGVIMNPTTVNGLSAYTLAGDQVNPETKATIRVMGYFIQYNNLIYQILGL
                     STPQDMAAYMPYFEHTMRNFRALTDASKINVTPERVKIKSVLVDGTLEQTLRAYNMPE
                     ARLQELSILNGMELKDRVTAGMLIKTVGK"
     assembly_gap    3966..3997
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3998..4261
                     /locus_tag="PK198_21560"
     CDS             <3998..4261
                     /locus_tag="PK198_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41401.1"
                     /translation="NRAMARCKLQAALSTAYLIPHTAYRTFALSTAHRELPTAYRPNR
                     AQCYGIRRKFKPNGYICTSVVFLIHPTIAMPNLAEIIQQQATS"
     gene            4258..4884
                     /locus_tag="PK198_21565"
     CDS             4258..4884
                     /locus_tag="PK198_21565"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRF41402.1"
                     /translation="MTYLFEGVKVGLLISLLAGPILFTLLQTGIEKGLRAGLAVGSGI
                     WISDVLFIVAVYYGLSYVKNFVSGPAFVLTLGIVGGALLIIFGLASLLSKPLQAIPQQ
                     SSAGGAKHWTGLWLKGFLINTINPFTFFFWIGIAGRVVVDGGLSPQQSFQYFAGIVGA
                     IVLTDSLKVALAKRIRRILRPAHLLWMRRIAGIGLIAFGIALMVRVLW"
BASE COUNT         1122 a         1359 c         1278 g         1163 t
ORIGIN      
        1 gatacgagcc gcagttgtca cttacaaaaa cagcgttgcg aaagtctttc agagtataat
       61 cgcaagtttc atctacaaat ccttccatcg aaaaagggca gaataccaat ggcggacgat
      121 tgtcggtacc caggccgcaa aggaaattgc agggaggcgg caggacgaag cggatattgt
      181 cattggtgag ccagttggac tgctgataca aggcagccct caatacagct acggtggggc
      241 cggtcacaga gcagttgtaa cgagcgttgt ccacttgtgg gctgatggcc agcgcatgaa
      301 cgccatcggt gagaccgggc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcatcggt
      361 ttgccaaaga ccgttcatgt gcagggccgc aatgaatttg gcctgctcgg cgggcgagtt
      421 gttgaatggc tctgagcctt gatatgcaaa aatctgatct cctgatgcag agagcaggat
      481 attagcgccg gagctgcttc ctgcgctgag gccgtcaaaa tcgaggaaga cgcccatgat
      541 ggccggcacc cgaaattgct ctcccaccac ccgggcagaa gaaaaagtaa tggtcatggt
      601 gctttcgccc ggcctgaagc cgcccgctgc cgtccagccc ttgtcggtaa aggtaagggt
      661 ggttcccgcc gagacgtctt tgagtagtac aaaggaaaaa tcatcaggat tgtcgctgtt
      721 gtaaccggta aaagcgatgt cgccggcact cagcgtggtt tgggttgccc cgatctgcca
      781 aaaaagcagc aggcaaaggg cagccgggat tatgcgggct gccgcagtgt gttgatatgt
      841 tctcatagta gtaaaaaatt ggtatgcaga atgtgttgtt cctaaaaccc ttcatcaggc
      901 tgcacacatt ttcagtgata caagctgacg cattgaaacc cagatagcta ctctttgaaa
      961 cgaagcgcgc cgggaggatg cacgcttttt ttattgatcg gttaagggga aaagaatgtt
     1021 attcctttca gcaagcaaaa atacagtact ttcatctata tttgcaacct aaagagcaag
     1081 attatgaaag acaatttttg tgtttactta gacgccggtc atggcgctat tgatcctaaa
     1141 ggcaattaca ccactgcgcc ttctaaaatg tttgaccaca aaaccggtac attccacaaa
     1201 ggatcagtgt tttatgaagg cgtttggaac cgtgcgctcg tcaatcgggt atcggccaag
     1261 ctacaaacgc tcggcatccg ccacctgatg gtacaccacg aatatttaga cacgccgctc
     1321 cagcaacgcg tggataccgc caactggtac cacaaaaatt ttgccgaggg catttatatt
     1381 tccacgcact ccaacgcagc gaactcacag gcaagaggtt ttgaggtata tacttccaaa
     1441 ggccaaacgg cttccgaccc cattgccacc atgatgtaca accacgttgc caacctgctc
     1501 ggcaaccgca tcaccatgcg ccccgacaac agcgacggcg atcccgaccg cgaagcggcc
     1561 ttctttgtac tgaccaaaac agccatgccc gccattctca tcgaacactt gtttttcgac
     1621 aatctggaag atgcccggct gttgatgaac gacgatatcg tggacttgtt cgcagaggct
     1681 caggtgcgta ccatcgttga acacttttcc aagtaattcc cgttgttgaa agatactttt
     1741 ctgacgcttg ccgcccctgc tcttggcgaa ttcaaagaca ggggcagcaa gttcattgcc
     1801 tacgcattcc ccatcgaatc tgaatccgac tggcaccaac gcctcgaaga agtgcgcaaa
     1861 gagcacccca aagcgaggca ctactgcttt gctttccggc tgggccagga cgggctgctc
     1921 taccgcgcca atgacgatgg cgagccggcg ggttcggcag gccgacctat actcggacaa
     1981 attgactcct tcgggttaac caatgttttt gtagttgttg tgcgctattt cggcggcacc
     2041 ttgctgggag ttccgggctt gattgctgct tacaaagaga gcactcagga cgcgctgcaa
     2101 caagctgaaa tcgttgaaaa aatcatcgtg caaacttttc gggtgcagta tgattatggc
     2161 ctgacgagcc agataatgaa tgctgtcaaa aaactgtctt ttgaattggc agagcaggaa
     2221 tttggcgata ccggcgctac ccttgaaatg cgcatccgaa aaagcgaggc cgatcaaaaa
     2281 atactggaat tcaaagcctt agcaggcaat ttacacttgg aagaggctgc tgttgtggag
     2341 gaaattccgg gcttgatgat agaatgggtg tagtttcttt agtttagttg gtgaaggggc
     2401 gactcagtca agaaaacagt ccccccttca ctatgtatag aaatgaaccc cttactttcc
     2461 taccgtctta atcaacatgc cggccgtcac gcggtctttc agttccatgc cgttgaggat
     2521 ggacaactct tgcagccgag cttccggcat gttgtaggcg cgcagcgttt gctccagcgt
     2581 gccgtccacc aacacagatt tgattttgac gcgctccggc gtaacgttga ttttcgaggc
     2641 atcggtcagg gcgcggaaat tgcgcatcgt gtgctcaaag tacggcatgt aggcggccat
     2701 gtcctgcgga gtagagagac ctaaaatctg gtaaatcagg ttgttgtatt ggatgaaata
     2761 ccccatcacg cgaatcgttg ctttggtttc cgggttcacc tgatcgcctg ccaaagtgta
     2821 agccgacagc ccgttcaccg ttgtcggatt cataatgacg ccggtcaatt gggcctcctg
     2881 agccagtccc tgagccgcct cctgcaatgt cttggcctgc gacaaactca tcaccatcag
     2941 ggctttgccg tctttgggcg ccatctggaa ctgctgtggc gagttttcgt gctgccagtc
     3001 ggcaggcacc ggaaactgaa acttcagcac gggatggtaa aaaacgccgc cttccacata
     3061 accctgacgc gggtcttctc catatatgat gttgtcaatc atgcgcaggt agctgtcgcg
     3121 gcccaccgca tattggccgg gccgggcctg ctgccatttg gcggcatcgg cggctacttt
     3181 tacattgcgg tcgcccggat cggggtgcgt agagaggaag gtgggcaccc ggccgccgcc
     3241 ctgatcgctg aggcgctgga gggttttgaa aaacccggcc atttcgttgg catcgtagcc
     3301 gattttcgag gagtattcta cgcccagtcc atcggattgt gtttcatggt cgcggccaaa
     3361 cttgaggaac agcaacccca tgccggtttg cgcggcgcct gctatttttc tgaagtcttc
     3421 cgaaatgatg acgcccgcca tcaggcccac ctggcccagc atttggttgg aatattgctg
     3481 cgccgagtgc cgcgccgtga cgtgcccgat ttcgtgaccc aatacacctg caaactccgc
     3541 ctcgttgttg aagtgcgcca tgatgccccg cgtgaaatac acaaaaccac ccggcacggc
     3601 aaaggcattg actaccggag agtccacgat tttaaattcg tagcccaggt tcggccgatg
     3661 cgaaaccgcc gccatctgct tgcctttttc gttgataaat tgctgaagac cagcgtcttc
     3721 atacaagccc atgcccgcta tgatttccgg gtcggattgt atgccgaggg ctttttcctg
     3781 ttcttccgac atcaggatga gttgtttttt ccccgtcacc ggattgaccg cgcaggaaac
     3841 ggcgctgtgc aacaccacag ccaagagcaa aaatttgaga gtcgttttca tattttgatg
     3901 attgaatgat cttttaatgt tcaaggtttc gggttgcatc cgccgtagcc ttggcgaagg
     3961 tgggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac cgcgccatgg cgcggtgcaa
     4021 gctgcaagcc gcgcttagca ccgcctacct aataccgcac accgcctacc gaaccttcgc
     4081 gctgagcacc gcccaccgag aactgcctac cgcctaccgg ccaaaccgcg cacaatgtta
     4141 cggcattaga cgcaaattta agccgaatgg ttacatttgt acctcagttg tgtttttgat
     4201 tcatccgacc attgccatgc cgaatttagc agaaatcatt caacaacagg ccacatcatg
     4261 acctatctat tcgagggcgt caaagtgggc ctcctcatca gtttgctggc aggccccatc
     4321 ctcttcacct tgctccaaac cggcatcgaa aaaggtctgc gtgccggcct ggccgtaggt
     4381 tccggcatct ggatcagcga cgtgctgttc attgtggccg tatattacgg gctgtcttac
     4441 gtcaaaaatt tcgtctcagg tccggcattt gtgctcaccc tgggcattgt cggcggcgcc
     4501 ctgctcatca ttttcggcct ggcatcgctg ctcagcaagc ctttgcaagc catcccgcaa
     4561 caatcgtcag ccggcggcgc caaacactgg acgggcctgt ggctcaaggg cttcctcatc
     4621 aacaccatca accccttcac cttctttttc tggatcggca tcgccggccg cgtcgtcgtt
     4681 gacggaggac tttcgccgca gcagtcgttt caatacttcg ccggtatcgt cggcgccatc
     4741 gtcctcaccg attcgctcaa agtagctctg gccaaacgca tccgccgtat cctgcgcccg
     4801 gcacacctgc tctggatgcg ccgcatagcc ggcatcggcc tcatcgcgtt cggcattgca
     4861 ctcatggtca gggtattgtg gtgattgtca aaaatttggg cgtgccggcg caccaataga
     4921 aaccaatgcg ccgtcgggtg cctcggtcgt cctcctaacg tcgttcctcc cttcggcccc
     4981 ct
//
LOCUS       DAORLF010000366         4975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9259_length_4975_cov_2.435976, whole genome shotgun sequence.
ACCESSION   DAORLF010000366 DAORLF010000000
VERSION     DAORLF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9259_length_4975_cov_2.435976"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1157
                     /locus_tag="PK198_21570"
     CDS             45..1157
                     /locus_tag="PK198_21570"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF41403.1"
                     /translation="MTLHERLRSPGPKRILALDGGGIRGALTLGFLEKLEALLRQRHN
                     NPRLLLCDYFDLIGGTSTGSIIATLLAVGKDTAFIKKMYLELGGEIFAQRRGIFGMLS
                     AKFKEDALRAQLKKHLGDRPFGDESLKTGLGIIAKRADTGGTWPLVNHPDMPYFDRNR
                     EMPLRNVVRASTAAPTYFLPEKINVGAGEEGAFVDGGVSMANNPALQLFLIATLPAYN
                     IRWATSADQLLVVSVGTGATRSKFKIDDVLDNRLWDWAAAVPNMLMYDANMQNQLILQ
                     TLARSPHAQKIDRALGTSVGGLLPEDRALLHYLRYDAPLNEEVMAEIGLPKSLAEIEK
                     LREMSDAENRFELAKIGEKAAAKYVKSEHLPASFDL"
     gene            1241..1744
                     /locus_tag="PK198_21575"
     CDS             1241..1744
                     /locus_tag="PK198_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41404.1"
                     /translation="MEAILSQEQMCALFIPLLQSRGSEYKKFRPIRAKAALDGERIET
                     RTSDGLETINTAEAGDMIVQNTTRAGEAYVMSADKFAERYVPAPEESTDNEGFALYHP
                     VGRILALCIDEALLQELQLPQAFHFTASWGSSMAAKAGDYLACPPDGSSVYRIAREEF
                     FETYQLV"
     gene            complement(1896..>2220)
                     /gene="rmuC"
                     /locus_tag="PK198_21580"
     CDS             complement(1896..>2220)
                     /gene="rmuC"
                     /locus_tag="PK198_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRF41405.1"
                     /translation="VIVTTSTLLATMRTVAFIWKQDKQKRSVMEIARQSCLLYDKFVA
                     FVEDLRGIGQRLDSAQQAYHDAMLKLSDARRPGDTLIGKAEKIRELGAKTSKQLPKEL
                     TDGGE"
     assembly_gap    2221..2233
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2234..3230)
                     /gene="rmuC"
                     /locus_tag="PK198_21585"
     CDS             complement(<2234..3230)
                     /gene="rmuC"
                     /locus_tag="PK198_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRF41406.1"
                     /translation="MEATLTISLLFLLIGLVIGALSAWMLARFKSRIDHLPKSEVQAQ
                     FVAKEVHEAALQQNDLLRDELISKEEQLRELTGQTRAQEQSMLHLREKLEEQRHEMLR
                     LQERFQTEFENVANRLLEEKSQKFSAHNQQQIGELLHPLREKIKTFEENIERRFVEET
                     RDRVSLKKEIEQLRDLNRQLSQDANNLAGALKGDNKAQGDWGEMQLELLLERAGLAKD
                     IHYSVQSSFADEEGRQKRPDCIIHLPDSKHLIIDSKVSLTAYERFFSSQEPEEQKKHL
                     AAHVDSMRRHIRELSEKNYTSLYQINSPDYLLLFVPLESAFNVALQQDSRLFLDAL"
     gene            complement(3233..4924)
                     /locus_tag="PK198_21590"
     CDS             complement(3233..4924)
                     /locus_tag="PK198_21590"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41407.1"
                     /translation="MEPRKIAEQVRQLIAQAETEEALRVLLEYLPTEKRWSELKQAAA
                     EAQAQFLNAKREANGGRITFDQADLIYNRVNNAVYQIADDLENGITRLTPAQQTRRML
                     PYLAIAIGAVAIAFLGYWVSQQGGEDDPADTQQTQTGCPDFEAGDSFKILLWEYDSFN
                     EDNVDSKKLPVALQNRLSSIDRDGLLQTGIYTLAENQKYPLRKEQALQCEAQLSLWGT
                     TDKVNENQWIVITNFAFAKPWQLKRWGMSPGDDWEKSDIAATLPLQGVFVDTISSLSS
                     VFTEGKISTKVEQLLQIAIGLNATLTSDQNGAVATLESFESQDSTLALLGGMLLADNY
                     TRMGKEEQALAAYEKVLSVHPNYALALNNYAALQIKKGNYGEAIATLDNAATVAPKNA
                     DVLAMRGSAYLAVNQLDKAQKDFSKAQELTTNKAAGTEARKEVRSKVIEQKVEELNVR
                     VQQEKQRRSEAEQQLKVTPENVEALNTRADASKNLGDHKTAIQSANEVIKMQPDNVKA
                     HTILVESLTALGDTARVRTALTRAEKIGIAPAEIKENAPLIRSLTDTTLFRRRFN"
BASE COUNT          999 a         1359 c         1373 g         1231 t
ORIGIN      
        1 cgaaatcggg aatccgaaat attgaatctt aaaatccata gaagatgaca ctacacgaaa
       61 gattgagatc gccgggaccc aaacgcatcc tggcattaga cggcggcggc attcgcggcg
      121 cgctcacgct gggctttttg gaaaaattgg aagccctgct ccggcagcgg cacaacaatc
      181 ccaggctcct gctctgcgat tatttcgacc tcatcggcgg caccagtaca ggctccatca
      241 ttgccaccct gctggcggtc ggcaaagaca cggccttcat caagaagatg tacttagagt
      301 tgggcggcga gatttttgcc caaaggcggg gcatcttcgg tatgctcagc gccaaattca
      361 aagaagacgc gctccgggcg caactcaaaa agcacctcgg cgaccgcccc ttcggtgatg
      421 aaagcctcaa aaccggcctc ggcatcatcg ccaaacgcgc cgacacaggc ggcacctggc
      481 ccttggtgaa ccaccccgat atgccgtatt tcgacagaaa cagagagatg ccgctccgaa
      541 acgtcgtgcg ggccagcacg gcagcgccca cctattttct gcccgaaaaa atcaacgtgg
      601 gcgcggggga ggaaggcgct tttgtggacg gcggcgtgag catggccaac aacccggccc
      661 tgcaactctt tctcatcgcc accctgcccg cctacaatat caggtgggcc accagcgccg
      721 atcagttgct cgtcgtttcg gtgggtaccg gcgccacccg ctccaaattt aaaattgacg
      781 acgtactcga caacaggctc tgggactggg ctgcggctgt tcccaatatg ctcatgtacg
      841 atgccaacat gcaaaatcaa ttgatattgc aaacgctggc ccgctcgccg cacgcgcaga
      901 aaattgaccg cgcattgggc acttccgtcg gcgggttgct gccggaagac agggcgctgc
      961 tgcattatct gcgctatgat gctcctctga atgaggaggt tatggccgaa atcggtttgc
     1021 ccaaatcgct cgccgaaatt gaaaaactga gggaaatgag cgacgctgaa aatcgcttcg
     1081 agttggcgaa aataggcgaa aaggcagcgg caaagtacgt caagagcgag catttgcccg
     1141 cttcgtttga tttgtaaagg ttctaccctt agtgaagggg tgacttcagc attcaacctg
     1201 acggagtcac cccttcacta tctatcaata ataatacacg atggaagcta ttctgagtca
     1261 ggaacaaatg tgtgcgctgt tcattccgct gctgcaatcc cgcggcagcg agtacaaaaa
     1321 attccgcccg atccgcgcca aagcagccct tgacggcgag cgcatcgaaa cccgcacctc
     1381 cgacggattg gaaaccatca acaccgccga agccggggat atgatcgtgc aaaacaccac
     1441 ccgagccggc gaggcttatg tgatgtctgc cgataaattt gccgagcgct atgtacctgc
     1501 tcccgaagaa agcactgaca atgagggctt tgcgctgtac caccccgtag gtcgcattct
     1561 ggcgctgtgc atagacgagg cattgctaca ggagcttcag ttgccgcagg cgtttcattt
     1621 caccgcatcc tggggcagca gcatggccgc caaagccggc gattatctgg cctgcccgcc
     1681 cgacggcagt tcggtgtatc gcatcgcgcg ggaagagttt ttcgagacgt accagttggt
     1741 gtagtttttt tttgtttttg ggcgcacccc actctaccgc ctccccgacc ctccagagga
     1801 gggagcatca gggcgtgggg ccgggctgtc cgctacatgc gcctctccgc tgtgaaccgg
     1861 taaggcccat ccgcttccat ccctcgcgca acgggctact ctccgccatc cgtcaattct
     1921 ttcggcaatt gtttggaggt ttttgctccc aactccctga ttttctcagc cttgccaatg
     1981 agcgtatcgc cagggcggcg ggcgtcgctc agcttgagca tggcatcgtg gtaagcctgt
     2041 tgcgccgaat ccaagcgctg gccgataccg cgcaagtctt ccacaaaggc cacaaactta
     2101 tcgtacagca ggcagctttg tcgcgctatt tccatcaccg agcgcttctg cttgtcctgc
     2161 ttccagatga aggccaccgt gcgcatggta gccagcaggg tagaggtagt gacgatgacg
     2221 nnnnnnnnnn nnncgagcgc gtcgaggaag agccgcgaat cctgttgcag cgccacgttg
     2281 aaggccgatt ctaagggcac aaacagcagc aggtagtccg ggctgttgat ctgatacagc
     2341 gaggtgtaat ttttctcgct cagctcgcgg atatggcggc gcatactgtc cacatgcgcg
     2401 gccaagtgct ttttctgctc ctccggttcc tggctgctga agaagcgctc gtaggccgtc
     2461 agcgacactt tggagtctat gataaggtgc ttgctgtcag gcagatggat gatgcaatcg
     2521 gggcgttttt ggcggccctc ttcatcagca aaggagcttt gcaccgagta gtgaatgtct
     2581 ttggcgaggc ccgcccgttc cagcagcagt tcgagttgca tttcgcccca gtcgccctgc
     2641 gctttgttgt cgcctttgag cgcgccggcc agattgttgg catcctgact gagttggcgg
     2701 ttgaggtcgc ggagttgttc tatttctttt ttcagcgaga cgcggtcgcg ggtttcttcc
     2761 acaaagcgcc gctcgatgtt ttcctcaaag gttttgattt tttcgcgcag cgggtgcagc
     2821 agttccccta tttgctgctg attgtgtgcg ctgaacttct gcgatttttc ctccagcagg
     2881 cggttggcca cgttttcaaa ctccgtctgg aagcgctcct gcaagcgcag catctcgtgt
     2941 ctttgctcct ccaatttttc ccgcaggtgc agcatagatt gctcctgcgc ccgcgtttgg
     3001 ccggtcagtt cgcgcagttg ttcctctttg gaaatcaatt catcgcggag gagatcgttt
     3061 tgttgcaagg cagcctcatg cacttctttg gccacaaact gagcctgcac ttccgacttg
     3121 ggcaggtggt caatccgcga tttgaaacgg gccagcatcc aggcgctgag ggcgcctatt
     3181 accaagccaa tgagcaaaaa aagcagggaa atagtcaatg ttgcttccat gttcaattga
     3241 atcttcggcg gaataaagtg gtgtccgtga ggctgcggat gagcggcgca ttttctttga
     3301 tttcggcagg agctatgccg attttttcag cgcgtgtgag ggcggtgcga actctggcgg
     3361 tatcgcccag cgcggtgagt gattccacga gaatggtgtg cgctttcacg ttgtcgggtt
     3421 gcatttttat cacctcattg gcgctttgga tggccgtttt gtgatcgccc agatttttgc
     3481 tggcatcggc gcgggtattg agcgcctcca cattttccgg agtgaccttc agctgctgct
     3541 cggcctcgct gcggcgttgc ttttcctgtt gcacgcgaac gttgagttct tccacttttt
     3601 gctcaatcac tttagaccgt acctcttttc ttgcctccgt gccggctgct ttgttcgtag
     3661 tcagttcctg tgcctttgag aagtcttttt gagccttatc caattgattg acagccagat
     3721 aagccgagcc tcgcatggcc agcacatcgg catttttcgg tgccacggtg gctgcgttgt
     3781 ccaaggtggc gatggcctcg ccgtagttgc cctttttgat ttgcagggcg gcgtagttgt
     3841 tcagagccag cgcgtagttg ggatgcaccg acaaaacctt ttcataagct gccagcgcct
     3901 gttcttcttt tcccatccgc gtgtaattgt cggccagcaa catgccgccc agcagcgcca
     3961 atgtagaatc ctggctttca aacgattcta atgtggcgac agcgccgttt tggtcggatg
     4021 tcagcgttgc attgaggccg atggcgattt gcagcaattg ctccactttg gtagaaattt
     4081 tgccctcggt gaacacgctg gaaagcgagg aaatcgtgtc cacaaaaacg ccttgaagcg
     4141 gaagagtggc ggcaatgtcg gatttttccc agtcgtcgcc gggcgacatg ccccaacgtt
     4201 tgagctgcca aggcttggca aacgcaaaat tggtgatgac gatccattga ttttcgttca
     4261 ctttgtctgt tgtaccccaa agtgacaatt gtgcctcgca ttgcagcgct tgctcttttc
     4321 tcaacggata tttctgattt tcagctaaag tatagatgcc ggtttgcaac aagccgtctc
     4381 tgtctatcga actgaggcgg ttttgcaggg ccaccggcaa tttttttgaa tcaacgttgt
     4441 cttcgttgaa agaatcatat tcccacaaaa ggattttgaa agaatcgccg gcctcaaaat
     4501 caggacagcc ggtttgcgtt tgctgcgtat ctgcgggatc gtcttcgccg ccttgttggg
     4561 atacccaata tcccagaaat gcaatggcca ctgctcctat cgcaattgcc agataaggca
     4621 gcatgcgccg ggtttgctgt gccggcgtca ggcgggtgat gccgttttcg aggtcgtcgg
     4681 caatttgata tacggcgttg ttgacgcggt tgtaaatcag atcggcctgg tcaaaagtga
     4741 tgcgcccgcc attggcctcg cgtttggcgt tcagaaactg ggcctgcgct tcggcggcag
     4801 cctgtttcag ttcgctccag cgcttttcgg tgggcaaata ttcgagcagt acgcgcaggg
     4861 cctcttctgt ttcagcctgt gcgatgagtt gtctgacctg ttctgcaatc ttgcgtggct
     4921 ccatgtggga ggtgtttttc gagtgttgtg tgttgcagga aacgctctgt attgg
//
LOCUS       DAORLF010000367         4959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9304_length_4959_cov_1.785685, whole genome shotgun sequence.
ACCESSION   DAORLF010000367 DAORLF010000000
VERSION     DAORLF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9304_length_4959_cov_1.785685"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PK198_21595"
     CDS             complement(<1..585)
                     /locus_tag="PK198_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41408.1"
                     /translation="MKKRLLFTIIASLAAIAFLHAQVAPTVFAQDFTSGLIGINIDAD
                     GNIWATENGTGNDDGRITIIDQQGNKTVFMTGLPSTFIQAAGEIVGSYRTYQRPDNKV
                     LIVVGEGDHDLSETLLIVDKSDFSPGNPLTLEDVELVIDHGAFIHGLGFVQSNPFNVE
                     WDTEGNMYIADAGANSIVKWEAATGDFSIVKTLDR"
     gene            complement(631..2208)
                     /locus_tag="PK198_21600"
     CDS             complement(631..2208)
                     /locus_tag="PK198_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41409.1"
                     /translation="MIFLMPQALSGQSSFQVRFDTLPLAEMPRLHSFAFARHEGKWLL
                     LGGRQDGLHPKFGGFNTASANTSIWVVSPETAQVWSRSITELPDTLQEQLRSANMEFY
                     HHDASLLFAGGYGYSDLAQNHITYPYLTVIDVPGLVQSVVEGGPLLPHFHQMRDTFFA
                     VTGGQIQELADTFYLVGGHLFNGIYSANSDNPTQRYTAAIRKFTIENQAGTPQITHQS
                     EQIDELNLHRRDYNLSPHIFENGDQGLMAFSGVFQPGLALKPFLNIVAIRSGGHHSVN
                     GFSQFLTNYHCARVPVYSASENQMHTLFFGGMSEYWLSDTDSLLRDSRLPFVKTAARV
                     TRLPDGSYEEVAFDAELPYYTGTGAEFILADNTPTLGEEMVNYDALSDGSHLLGYIVG
                     GIVTPGSQRNPFTANAAAVTSASPQVLRVWLEKGAVSSTKGQALDGRYALQMRVFPNP
                     AEDVLQLSARLPRAGRLLGTLQNAQGRIVMSHDWGLCNEGLQQMEISAAALPAGLYWL
                     TLRLDGGFTETITIAKK"
     gene            complement(2551..>4519)
                     /locus_tag="PK198_21605"
     CDS             complement(2551..>4519)
                     /locus_tag="PK198_21605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator transcription factor"
                     /protein_id="HRF41410.1"
                     /translation="ANGKELPALDLLKELKIKHNQNNLSFEFTVLDFTDPAKNRYRYR
                     LVGADPDWVETGTYRFAHFTHLAPGRYTLLAEGSNGEGNWQPIHHPISIVIRPPWWSS
                     RPAYFSYFLSITFLVWGAYRFQVRRVKEREQLAFEHRETQRLKALEEMKSNFFNNITH
                     EFRTPLALIREPARRIRSQSDEAETIAHAQRIETNSLRLLDMVNQLLDLAKLESGSMS
                     TDLRRGNLEEATREWVQAFLPLADQRGIQLSFAAKGPLHAVVFDFKKTELIVNNLISN
                     ALKFTPQGGKVRVSASHSEGKAGVEIVVSDTGVGISEKDQLRIFDRFYQAENASSQQV
                     KGSGIGLALSKELAELLGGRLDVQSSLGKGAIFTLYLPESTAVEVQNPTVKKEQHVEV
                     AAVSTSSNYSDNLDKPIALLAEDNAELRAFVQECIGDRWQVVEASNGAEGIEKARLLV
                     PDIIISDVMMPLKNGFELCEALKNDELTAHIPIILLTAKSAVDSKITGLRTGADDYLT
                     KPFNTEELLARMANLTEQRRRMRLKYAQERPAAAPAGIPDHVSEPDKEFMQRFLALVE
                     QHLSDENLSVEELARQMLISRVQLHRKLKALTDQNVTDFVRDYRLDRAMAMLRNQEGL
                     VYEVAYKVGFGSEKYFSRAFKEKFGVSPSQV"
     assembly_gap    4520..4574
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4575..>4959)
                     /locus_tag="PK198_21610"
     CDS             complement(<4575..>4959)
                     /locus_tag="PK198_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41411.1"
                     /translation="NSIACLLPDPQRPNDILWIGTKGGGINRLDMRSGRITHLHTRQG
                     LPDNVVYGILPGQPNELWCSTNRGLARIRLNEQREPQTITAFTAAQGLQDNEFNTQAF
                     FKAANGELLFGGVNGLNQFFPEEVLP"
BASE COUNT         1039 a         1266 c         1405 g         1194 t
ORIGIN      
        1 gcgatctaat gttttgacga tgctgaaatc gccggtagca gcctcccatt ttacaataga
       61 attggcgcct gcgtcggcaa tatacatgtt gccctcggta tcccactcca cattaaatgg
      121 attggattgt acaaatccca ggccatgaat gaacgcgcca tgatctatga ccaattccac
      181 gtcttccagt gtcagcgggt ttccggggct gaagtccgat ttgtccacta tcaacaaggt
      241 ttccgacaga tcatgatcgc cttcgcctac cacgatgagc actttgttgt cggggcgttg
      301 ataggtgcga taggagccta cgatttcgcc tgccgcctga ataaaggtgc tgggcaggcc
      361 ggtcataaat acagttttgt tgccttgctg atctataatg gtgatgcggc cgtcgtcatt
      421 gccggtgccg ttttcggtgg cccagatatt gccgtcggcg tcaatgttga taccaatgag
      481 gcccgatgtg aaatcttgtg caaatacagt aggcgccact tgagcgtgca ggaatgcgat
      541 ggctgccaaa gaggcaatga tggtgaagag taagcgtttt ttcatggtca aaaaaggttt
      601 taatgtgatg atgaaaaatt gagtgtgatt ttactttttg gcaatggtga tggtttcggt
      661 aaaaccgccg tcgagccgga gcgtgagcca atacaagcct gcgggcaatg ctgctgccga
      721 aatctccatc tgttgcagcc cttcgttgca caggccccag tcgtggctca tcacgattcg
      781 gccctgcgca ttttggagcg tacccaagag gcgacctgcg cggggcaatc gggcgctcag
      841 ttgcagcaca tcttcggcgg gattgggaaa caccctcatt tgcaaggcgt agcgcccatc
      901 caatgcctgc cctttcgtgg aggataccgc gcctttttcc aaccacaccc tgagtacctg
      961 cgggctggcc gaagtaaccg ctgcggcatt ggcagtgaat ggattgcgct ggctgccggg
     1021 cgtcacaatg ccgcctacaa tgtagccgag caggtggctg ccatcggaga gcgcatcata
     1081 gtttaccatc tcctcaccca gcgtgggcgt gttgtcggcg aggatgaact cggccccggt
     1141 tcctgtgtag taaggtagtt cggcatcgaa ggcgacttct tcgtaagagc cgtcggggag
     1201 gcgggtcact ctggcggctg ttttcacaaa aggcaagcga ctgtcgcgca gcaagctgtc
     1261 tgtgtcggag agccagtact cgctcatgcc gccaaaaaac aaggtgtgca tttggttttc
     1321 cgaggctgaa taaacgggta cgcgggcgca atggtaattg gtcaaaaact ggctgaaccc
     1381 attgacggag tggtgccctc cggagcgaat ggccacgatg ttcaaaaagg gtttgagcgc
     1441 caggccgggt tgaaacactc ccgaaaaagc catcagcccc tggtcgccat tttcaaaaat
     1501 gtgaggcgaa agattgtagt cgcggcggtg cagattgagt tcgtctattt gctcgctttg
     1561 gtgtgttatt tgtggcgtac cggcttggtt ttcaatggta aacttgcgaa tggccgcagt
     1621 gtagcgctgt gtggggttgt cggaattggc cgagtaaatc ccgttgaaca aatgtccgcc
     1681 caccaaataa aaagtatccg ccaattcctg aatctgaccg ccggtaacgg caaagaaggt
     1741 atcccgcatt tgatgaaaat ggggtaggag agggccgccc tccaccacgc tttgcaccaa
     1801 gcccggcaca tctatgacgg tgaggtatgg ataagtgatg tggttttggg ccagatcgct
     1861 atacccgtag ccgccggcaa aaagcaggct ggcatcgtgg tgataaaact ccatgttggc
     1921 gctgcgcaac tgctcttgca gggtgtcggg cagttccgtg atggagcggc tccacacttg
     1981 tgcggtctcc gggcttacca cccagatact tgtattggcc gaggccgtat tgaaacctcc
     2041 aaatttggga tgcaaaccat cctgccgacc gcccagcaac agccacttcc cttcgtgccg
     2101 cgcaaaagca aaggagtgca gcctcggcat ttcggccaag ggcagggtat cgaaccgcac
     2161 ctgaaaactg ctctggccag acagtgcctg tggcataagg aatatcatcc acaagaaaaa
     2221 ggtctgcgcg cccactgcga ccgcgcagac gaaatctaaa aaatgagttg tatatgtaat
     2281 agtcggcttc ataatccatt tgtgttctgg cagggacaaa attgggttgt atgaagccga
     2341 tacggagtgc tctgtcgtta cattgtggta tcctgaggtt acattttggg ccggggtgcc
     2401 ctgaggttac atgggggtgt aatgaggtta catgtgggaa cggggattgt ttcgggttca
     2461 aggtttcggg tttcatccgc cgaagccttg gcgaaggcgg gttcgaggtt ccgggttgcg
     2521 ccgcccaccg cctaccggtt cccctcaccc ctacacctga ctcggcgaaa cgccgaactt
     2581 ctctttaaaa gccctggaga aatacttttc actgccgaac cctaccttat aggccacttc
     2641 atacaccagc ccttcctgat tgcggagcat ggccatggcc cgatctaagc ggtaatcccg
     2701 cacaaaatcg gtcacatttt ggtcggtcag cgccttcagc tttctgtgca gttgcaccct
     2761 gctgatgagc atctgccggg ctagttcttc cacactgagg ttttcatcgg agaggtgttg
     2821 ttccaccaaa gccaaaaatc gctgcatgaa ctccttgtcc ggttctgaaa catgatccgg
     2881 tatgcctgca ggtgctgctg cggggcgctc ctgagcgtat ttgaggcgca ttcggcggcg
     2941 ctgctctgtg aggttggcca tgcgcgccag caactcttcg gtattaaaag gcttggtcag
     3001 gtagtcgtcg gcgccggtgc gtaaaccggt tatttttgaa tctactgccg atttggccgt
     3061 gagcagtatg atgggaatat gcgctgtgag ttcgtcattt ttcaaggctt cgcaaagctc
     3121 gaacccgttt ttgagcggca tcatcacgtc agaaatgatg atgtcgggca ccaacaggcg
     3181 agccttttct atgccctctg ctccgttgga agcctccacc acctgccacc tgtcgccgat
     3241 gcactcctgc acgaaggctc tcagttcggc attgtcctcc gccaacagtg cgatgggttt
     3301 gtctaagttg tcggaatagt ttgaggaggt cgaaacggca gcaacttcta catgctgttc
     3361 ttttttaaca gttggattct gcacctcgac agcagtgctt tcgggcagat acagggtaaa
     3421 aatggccccc ttgccgagac tgctttgcac atccaggcgg cctcccagca actcggccag
     3481 ttccttactg agcgccaatc ctatgccgga gccttttact tgctgtgagg aggcgttctc
     3541 ggcctgatag aagcggtcga aaatgcggag ctggtctttt tcagaaatgc ccacaccggt
     3601 atcagacacc acgatctcca cacctgcctt gccctctgaa tggcttgcgc tcactcttac
     3661 tttgccaccc tgtggagtga atttcaatgc gttggaaatc agattattga cgattaattc
     3721 tgttttttta aaatcaaaaa ccacggcatg gagcgggcct tttgcagcaa aagaaagctg
     3781 tatgccccgc tgatcggcca agggcaaaaa cgcctgcacc cattctcgcg tggcttcctc
     3841 caggttgccg cgacggaggt cggtgctcat gctgccgctc tccaatttgg ccagatcgag
     3901 cagttgattc accatgtcca acaatctcaa actgttggtc tctatgcgct gcgcatgcgc
     3961 gatggtttcg gcctcgtccg attgggagcg aatgcggcgg gccggttccc gaatcaaagc
     4021 gagcggcgtg cgaaactcgt gggtgatgtt gttgaaaaaa ttgcttttca tttcctccaa
     4081 tgccttgagg cgttgtgttt cgcggtgctc gaaggccaac tgttcgcgtt ccttcacccg
     4141 tcgtacctga aaacggtagg cgccccatac caaaaatgtg atgctgagaa aatagctgaa
     4201 ataggctggc cgggaactcc accagggcgg gcgtatgacg atggaaatag ggtgatggat
     4261 aggctgccag ttgccttctc cgttgctgcc ttccgcgagc agggtgtatc gtcccggagc
     4321 cagatgcgta aagtgcgcaa agcgataagt gccggtttct acccagtcgg ggtcggcgcc
     4381 cacgaggcgg tagcgatagc ggtttttggc ggggtcggtg aagtccaata cggtaaattc
     4441 aaaagacagg ttgttttggt tgtgcttgat tttcagttct ttgagcaggt ccaatgccgg
     4501 caattccttc ccattggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnngggcaa tacttcttcg gggaaaaatt gattgaggcc gttgacgcct
     4621 ccaaaaagga gttccccatt ggccgcttta aaaaaggcct gggtattgaa ctcgttgtct
     4681 tgcaagccct gagcggcggt gaacgcggtg atggtttgcg gctcgcgttg ctcattcagt
     4741 cttatgcggg ccaggccccg gttggtgctg caccagagtt cattgggctg gccgggcagg
     4801 atgccataca ctacgttgtc gggcaggccc tgccgcgtat ggaggtgcgt gatgcgcccg
     4861 ctgcgcatgt ccaagcggtt gatgccgccg cctttggtac cgatccagag gatgtcgttg
     4921 gggcgctggg ggtcgggcag gaggcaggcg atggagttg
//
LOCUS       DAORLF010000368         4959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9306_length_4959_cov_1.567292, whole genome shotgun sequence.
ACCESSION   DAORLF010000368 DAORLF010000000
VERSION     DAORLF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_4959_cov_1.567292"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2621)
                     /locus_tag="PK198_21615"
     CDS             complement(87..2621)
                     /locus_tag="PK198_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41412.1"
                     /translation="MAARHFSPQTISLFLFIAISCCTASAIAQGWTRQFDLCRGERYG
                     NAIATADGGVVFAGIGFAGSEWYSDSLLLLLRVDAGGEEIWRKTDTMPHSLGVVLTEQ
                     LSDGNFAVFYAFKEDPPVRLHYQKRNPWGQIIAQQNDVAALQGPVYPSGRPFAIIRTE
                     TDDDILLSIGRNVLKMNGNGDMIWIAGLPTGNIATAAKKSAGNTYLIAGSGHDDRPFL
                     SRWSATGQMLWHHLYQEARGAGDICSIIEIENGNILFSAEWIDAALNDGSILKTDATG
                     ELISIATTSNGTISDMVALPDAQGFVAVRNGFGAVIPRAKFLRFDLDMQPNFEHEMEG
                     NHPPYLFLRKLIPAPDGGYYCLGSTNDIWSGEDFVFIKTDANGSVFSNRLRGSIRRDG
                     NADCMAESGELPVFEGFKLQLTNATDTFVRFPRYDGSYAFDVSPGAYLFSATPPPGSR
                     VWQACPPMPVTLAAPNTLSEQDIAITPLADCPELHLQMSAANVLRICHTYYPSLTCTN
                     RGSATAPSPQVRLVLPDSMEVLHSRPAISSQSGNVLIYNLNDLHPGEETNISLRLRSP
                     CDVANLGREACFSASVATDTACLPPLLPGNIHNGWSNVNFCREFRGPYDPNDKSAWPE
                     GVGEEHIIAPETRILYHIRFQNTGNDTAFLVVLRDTLSPQLDVRTLQLGASSHPYRLI
                     VSDEGELQFRFENISLPDSSANFEESQGFVQFSIVPRQDAPLGTQIFNSAGIYFDFNP
                     PIITNTVHHTLGRLSTAIQPVLPMQPEVTAAPNPALHEVLFSWKKHESQAFILDIWDA
                     SGRFVHRTEGHGDSFLWNRNRIPAGMYWYRLQLENAGMARGRLVLR"
     assembly_gap    2795..2885
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2940..>3132)
                     /locus_tag="PK198_21620"
     CDS             complement(2940..>3132)
                     /locus_tag="PK198_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41413.1"
                     /translation="EVVNVSRLKWDAGGQSVTFDVPLPQKEEYYLLFNWWGVQQPFQS
                     VKGIMLKASSGLVVKSNKN"
     assembly_gap    3133..3169
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3170..>4265)
                     /locus_tag="PK198_21625"
     CDS             complement(<3170..>4265)
                     /locus_tag="PK198_21625"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HRF41414.1"
                     /translation="ALIRIEYPETYELANVVLALTKYGITDKWQVRKDFAYYEEMRKY
                     FEPYMGHPLLDSVNFSGKRWQEYLSYRTDSYAFVLDENYKLQRINDFQSFEIQTFDQY
                     KSLTEDFARTSNFKAFFQSQQTYREQMMRKYREEYLLTEMKDFLTKEFGDYFGNKKYS
                     VVISPFVYAQNLHRDIDTAWTADFPTIAKPIIDGTSFENQEDKSTELHTLFTEMDHGY
                     VNPTTDQHNVAAGFDETLWDDDSGYANSGNAVFNEYMTWAVFDIFNSIHFPAFAEKVN
                     LNWHFQNDTRGFIYSDLFAAKLKALYKQYNGRKKIKDLYPEMLKWTKDIQPNLSKPLI
                     LNDSDTIHVTSPINSIRLEFSEPMKPVETVD"
     assembly_gap    4266..4322
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4608..4658
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4659..>4959
                     /locus_tag="PK198_21630"
     CDS             <4659..>4959
                     /locus_tag="PK198_21630"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41415.1"
                     /translation="PADSLAPLYLFKSAGVAKTTGQFYEAYRLWDKLLAEYPDNFWAP
                     PAAFLKGYTAETELMDRDKAVTYFEEFLKRYPESNFAAQAQRQIDLLKGNVKPEDL"
BASE COUNT         1147 a         1169 c         1200 g         1207 t
ORIGIN      
        1 tagataagac acttaaaatg aaacgtttac ttatctaatc ctttgacgaa aatgaccact
       61 ttattttttt attgcccctt accctcctac ctcaacacca acctccctct cgccataccc
      121 gcattttcca actgcaaccg ataccaatac atgcctgccg gaatgcggtt tcgattccaa
      181 aggaaggagt cgccatgccc ctcggtacga tgtacgaaac gaccggacgc atcccaaata
      241 tcgagtataa aagcctgact ttcatgcttt ttccaactaa acagaacttc gtgaagggcc
      301 gggttgggag ctgccgtaac ttcgggttgc atcggcaaaa cgggctgaat ggccgtgctc
      361 aacctgccga gggtgtgatg caccgtattg gtgataatgg gcggattaaa atcaaaataa
      421 atgcccgccg agttgaaaat ctgcgtgccg agaggagcat cctgacgtgg tacaattgaa
      481 aactggacga aaccctggct ctcctcgaaa ttcgcgctgc tgtcgggcag gctgatgttt
      541 tcaaatcgga attgcaattc gccttcatcg ctcacaatca ggcgataagg atggctggat
      601 gcgcctagct gcaaagtgcg cacgtcaagc tgcggcgaca gcgtatctcg aagcaccacc
      661 aaaaatgccg tatcgttgcc ggtattttga aaccgaatgt gataaagaat ccgggtttcc
      721 ggcgctatga tgtgctcttc gcccacgcct tcgggccatg cgcttttatc gttggggtca
      781 taagggcctc gaaactcccg gcaaaagttc acgttgctcc atccgttgtg gatgttgccg
      841 ggcaagaggg gcggcaggca ggccgtgtcg gtagcgacgg aggcggaaaa gcaggcttcg
      901 cggcccagat tggctacatc gcaggggctg cgcaggcgga gcgaaatatt ggtttcttcg
      961 ccgggatgca gatcgttcag gttgtaaatc aatacattac ccgactgcga cgagatggcg
     1021 gggcggctgt gcaaaacctc catcgaatcg ggtagtacaa ggcgtacttg cggcgacggc
     1081 gctgtagctg agccgcgatt ggtgcatgtc aatgaagggt aatacgtatg gcaaatccgc
     1141 agtacgttcg cggcgctcat ttgcaggtgc agttcggggc agtcggcaag tggagttatc
     1201 gctatgtcct gttcgctgag ggtattgggg gccgcgaggg ttaccggcat cggcggacaa
     1261 gcctgccaga ctctgctgcc cggcggcggc gttgcgctga acagataggc gcccggcgaa
     1321 acatcgaagg catacgaacc atcataccgg ggaaaccgga cgaaggtgtc ggtcgcattg
     1381 gtcaactgta gtttaaatcc ttcaaaaaca ggcaattccc cgctttcggc catgcaatcg
     1441 gcatttccat cgcgtcggat gcttcctctg agccggttgc tgaataccga gccgtttgcg
     1501 tcggttttga tgaatacgaa atcttctcca gaccaaatat cattcgttga tcccaaacaa
     1561 taataaccgc catccggggc agggattaat tttcttaaaa aaaggtaggg tggatgattg
     1621 ccctccatct cgtgctcaaa attaggttgc atatcaagat cgaagcgcag aaattttgcg
     1681 cggggaataa ccgctccaaa tccatttctt acggcaacaa acccttgggc atcgggcagg
     1741 gctaccatat ccgaaattgt accgttagac gtggttgcga tagagatgag ttcccctgtt
     1801 gcatctgtct tcaaaatgct cccgtcattg agcgctgcgt ctatccattc tgcgctgaac
     1861 aagatgtttc cattttctat ttcgatgatg ctgcaaatat ccccggcgcc tcgtgcttcc
     1921 tgatacaagt gatgccacag catttggccg gtagcgctcc atcgtgataa aaaaggccgg
     1981 tcgtcatggc ccgagccggc tatcagatat gtattgccgg cacttttttt cgcagcagtg
     2041 gctatattgc ctgttggtag cccggctatc cagatcatgt ctccgtttcc attcattttc
     2101 aatacattac gaccaatgct caacaggatg tcatcgtctg tttctgtccg gataattgca
     2161 aaggggcggc ctgatggata aaccgggcct tgcagcgctg ccacatcgtt ttgttgggca
     2221 atgatttgcc cccagggatt tcgtttttgg taatgcaacc gaaccggcgg atcttctttg
     2281 aaggcataaa aaactgcaaa attgccgtcg ctcaactgct ctgtcagcac tacgccaaga
     2341 gaatggggca tggtatcggt ttttcgccag atttcctcgc cccctgcatc cactcgcagg
     2401 agcagcaaga gagagtcgga ataccattcg ctaccggcaa atcctatgcc cgcaaaaaca
     2461 acgccgccgt cggcagtagc aatggcattt ccatagcgtt cgccgcgaca aagatcgaat
     2521 tggcgggtcc agccctgcgc gattgcagat gcggtgcagc aactgatggc gataaaaagg
     2581 aagagtgaga tggtttgggg tgaaaagtgt ctggcagcca taatgttgtg ttttagtttt
     2641 ggaagctgcg aatataagca tgttatctaa aactacagca ttttgtggtg ttttgggaaa
     2701 cacctgacgg caatttccac tcctctgccc gaactacata ccgcagccat tcccgcccgt
     2761 cttcccggta ggcattacaa gccttgacgc tgctnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnngctgc atttcccgga ggtagaggcg gggcgtgcgg aggaggaagg gcatggattt
     2941 tagtttttat tgctcttcac caccaacccg ctcgatgcct tcagcatgat gcctttgaca
     3001 ctctgaaacg gttgttgtac gccccaccag ttgaacagca ggtaatactc ctctttttga
     3061 ggcaatggaa cgtcaaaagt taccgattgg ccgccggcat cccatttcaa gcggctcacg
     3121 ttcaccacct ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tccacagttt
     3181 caaccggctt catgggttcc gaaaactcca ggcggatact attgatcggc gatgtcacat
     3241 gtatggtatc ggagtcgttc agtatcaagg gtttgctcaa attcggctga atgtctttcg
     3301 tccatttcag catctcaggg tacaggtctt ttatcttttt ccggccatta tattgcttgt
     3361 aaagcgcctt cagttttgct gcgaataagt cggaatatat aaatcctcgg gtgtcgtttt
     3421 gaaagtgcca gttcaaattc actttttccg caaaggcagg aaaatggatg gagttaaaaa
     3481 tatcaaacac ggcccaggtc atgtattcat taaagacggc gttgccggaa ttggcatatc
     3541 cgctgtcatc gtcccacaat gtttcatcaa aaccagccgc tacattgtgc tgatcggtcg
     3601 tgggattcac atacccgtga tccatttctg tgaacagcgt gtgcaattct gtggacttat
     3661 cctcttgatt ttcaaagctt gtgccgtcaa taatgggctt ggcaatggtc gggaagtctg
     3721 ctgtccaggc ggtgtcaatg tcccggtgca ggttttgtgc ataaacaaaa ggcgagatga
     3781 caacggagta ttttttattg ccgaagtagt cgccgaattc ttttgtcaga aaatccttca
     3841 tttccgtcag caggtactct tcgcggtatt tgcgcatcat ttgctcccgg taggtctgct
     3901 gcgattgaaa aaatgccttg aaattcgatg tgcgggcaaa gtcttccgtc aatgatttgt
     3961 attggtcaaa agtctgtatt tcaaaggatt gaaaatcatt tattcgttgc agtttgtaat
     4021 tttcatccag cacaaaggca tagctgtccg tcctgtaact caggtattcc tgccagcgtt
     4081 ttcctgaaaa attgacggaa tcgagcaagg ggtgccccat ataaggctca aagtattttc
     4141 tcatttcctc gtagtaggca aaatccttcc tcacctgcca tttgtcggtt atgccgtact
     4201 tggtgagcgc cagtaccaca ttggccaatt cgtacgtctc tgggtactca attcggataa
     4261 gggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nngcaaaggc gagaccgagc aggagaagac ctccgatttt gttttttgtt tgcatggctt
     4381 ttgattttca ggtgaagcaa ttagtgacag gaagatcacg gcagtcaacc gcaaaagttt
     4441 gaaaacacta tctttgccgc aaaaaaacga aaaatgaaat acgcagcagc attgattttt
     4501 gtggccacgc tgatggcctg cacccccaac gcggacaagt tgaaagccga attgaaggaa
     4561 atggacgatg ttttttctaa ggcatccagc atttccgacc tgaaaatnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tgccgattcg ctggcgcccc
     4681 tgtacctgtt caaatcggcg ggcgtggcga aaaccaccgg ccaattttac gaagcatatc
     4741 gcctgtggga caaactgctg gccgaatacc ccgacaattt ctgggcgccg ccggctgctt
     4801 ttctcaaagg ctataccgca gagacggaac tgatggacag agacaaggcc gtgacgtact
     4861 ttgaagagtt tttgaaacgt tacccggaaa gcaatttcgc agcacaggct cagcggcaga
     4921 ttgacctgct gaaaggcaat gtgaaacccg aagatttgg
//
LOCUS       DAORLF010000369         4958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9310_length_4958_cov_2.023863, whole genome shotgun sequence.
ACCESSION   DAORLF010000369 DAORLF010000000
VERSION     DAORLF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9310_length_4958_cov_2.023863"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>234
                     /locus_tag="PK198_21635"
     CDS             <1..>234
                     /locus_tag="PK198_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF41416.1"
                     /translation="VLEVQQHLGEDSVRTIAMDSTDGLTRGTKVTDSGAAITMPVGEE
                     IKGRLFNVVGEAIDGIGAVPKTAAAYPLHRKPPT"
     assembly_gap    235..336
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <337..1393
                     /gene="atpD"
                     /locus_tag="PK198_21640"
     CDS             <337..1393
                     /gene="atpD"
                     /locus_tag="PK198_21640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF41417.1"
                     /translation="FGGAGVGKTVLIMELINNIAKGYEGISVFAGVGERTREGNDLLR
                     EMIESNVIRYGDAFKHSMEEGGWDLSKVDKTALRQSQATLVFGQMNEPPGARARVALS
                     GLTVAEYYRDGDLNDATGGKDILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAT
                     EMGLMQERITSTRRGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIAALGIY
                     PAVDPLDSTSRILDPLIIGEEHYNCAQRVKNILQRYNELLDIIAILGMEELSDEDKLV
                     VHRARRVQRFLSQPFHVAEQFTGLKGVLVPIEETIRGFNMIMDGEVDEYPEAAFNLKG
                     SIDDVIEAGKKMLAEAE"
     gene            1424..1729
                     /locus_tag="PK198_21645"
     CDS             1424..1729
                     /locus_tag="PK198_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRF41418.1"
                     /translation="MKISILTPEREVFNGLVTSVKVPGTRGEFQILNNHAPIVSSLDA
                     GFIHVVTAQGEYRYFDDESGEIKTAAEPGRSLRFKIAGGFVEVLRDEVSLLVHGVRE"
     gene            1903..2712
                     /locus_tag="PK198_21650"
     CDS             1903..2712
                     /locus_tag="PK198_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF41419.1"
                     /translation="MKKILFPTDCSASAHHAFRFAVQLARQLDAVIDIMSVYHLPATD
                     ASSVPPSYIETMIEERRRQVEANVAKFLEAWPDAPIGQRFADYGIFTHQEIVDKATEG
                     SHELIVMGTHGVGNAVDKLLGSVTTHTIMQAPCPVLAIPPGAEWKPIRHIAYATDFEA
                     GDGHAVYNLMEFAKLMGAQVHFVHIHTDDKEIGMEELDRPEAHPEAFADFTIVHNPSV
                     MDGLDQYVRERSIDMVALFIPRRRIWERLFHSSFSKKMAFHTALPLLTFRA"
     gene            2728..4146
                     /locus_tag="PK198_21655"
     CDS             2728..4146
                     /locus_tag="PK198_21655"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRF41420.1"
                     /translation="MEPSTNPGEPRNPLWRSARWSALITVFTAGGSLLWMLVLTRLLS
                     PADFSTSALALALVGVVLLFLQNGLPGAIIFQQERRSGVLSTLFWSNLALGLAGTLLL
                     AVSSFGAGIFFEDLRLHQVLLTISPLPLLSAGGAVYKSIHLRDLNYRTVAVVELTAFT
                     AGVAASAWLVFSGHGYWAAVAQWALRYAAEAVLYGMAGLGLFSPMPPSRNFQNLRTHQ
                     QFAAGQWAERLTMYLNANIDTLLIAKLLGAEALGVYDVFKRLIARPTSMAGEWLDRFA
                     FPLMARSETPARIFFTNTRLLSILLAPMAVLGILLAGPLLQVLTGAAWQVELRTFQWL
                     VAAIAVAALAHPWDGLLAATGRLKQLSLANMAYAMLLSAGLWAGAVWGLEGAAAAQAV
                     LAPLWIVVGYSWLIEPVTGQGMVVFLRPPAKWIGAALLCAAPAWAWLQIQNTPAPVPT
                     LILAVLSFTACYGWAVWKWGLR"
     gene            complement(4150..>4507)
                     /locus_tag="PK198_21660"
     CDS             complement(4150..>4507)
                     /locus_tag="PK198_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRF41421.1"
                     /translation="LLSRKAYPGDATVTICHSRTRNLAEHTRRADIIVAAIGSADFIK
                     GDMVKDGVVIIDVGMNRVEDPAAKNGYRLTGDVDFASVAPKSSYFTPVPGGVGQMTVV
                     SLMLNTLKAGKREVYG"
     assembly_gap    4508..4546
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4547..>4958)
                     /locus_tag="PK198_21665"
     CDS             complement(<4547..>4958)
                     /locus_tag="PK198_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRF41422.1"
                     /translation="VAAGGKAPHLAAVLIGEDPASQVYVRNKVKSCEEVGFHSTLIRR
                     PADISQEELMGIVADLNQNPDIDGFIVQLPLPKHLNEEEITLAIDPRKDVDGFHPVNF
                     GRMAQGLPCYLPATPAGILEMLKRYEIPTAGKEVV"
BASE COUNT          974 a         1351 c         1380 g         1112 t
ORIGIN      
        1 gtactggaag tgcagcagca cctcggcgaa gacagcgttc gcaccattgc tatggactct
       61 accgacggcc tcacccgcgg caccaaagta acggacagcg gcgcggccat caccatgcct
      121 gtgggcgaag aaatcaaagg ccgcctcttc aacgtggtcg gcgaagccat tgacggtatc
      181 ggcgcagtgc ccaaaaccgc tgcggcctac cccctccacc gcaaacctcc taccnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc ggcggtgcag gtgtaggcaa
      361 aaccgtgttg atcatggagt tgatcaacaa cattgccaaa ggttatgaag gcatctccgt
      421 gtttgccggt gtaggcgagc gcacccgtga gggcaatgac ctcctccgtg agatgatcga
      481 atccaacgtt atccgctacg gcgatgcgtt caagcacagc atggaagaag gcggctggga
      541 cttgtcgaaa gtggacaaaa ccgccctccg gcaatctcag gctaccctcg tgttcggcca
      601 gatgaacgaa cctcccggcg ctcgtgcccg tgtggcgctc tccggtctga ccgtagccga
      661 atactaccgc gacggcgacc tcaacgacgc taccggcggc aaagacatcc tgttctttat
      721 tgacaacatc ttccgcttca cgcaggcagg ttccgaagtg tccgctctct tgggtcgtat
      781 gccttcggcg gtgggttatc agcccacgct ggctaccgaa atgggtctga tgcaggaacg
      841 catcacctct acgcgtcgcg gctccatcac ctctgtacaa gcggtgtatg tacccgcgga
      901 cgaccttacc gacccggcgc cggctactac ctttgcacac ttggacgcca ccacggtatt
      961 gagccgtaaa attgcggccc tcggcatcta tccggccgtg gacccgctcg actctacttc
     1021 ccgcatcctt gatccgctca tcatcggcga agagcactac aactgcgctc agcgtgtgaa
     1081 aaacatcttg cagcgctaca acgaattgct cgacatcatc gccatcctcg gtatggaaga
     1141 attgtcggac gaagacaaac ttgtggtaca ccgcgcccgc cgcgtacagc gcttcctctc
     1201 tcagcccttc cacgttgccg agcagttcac cggtctcaaa ggcgtgttgg tgcccatcga
     1261 agaaaccatt cgcggtttca acatgattat ggacggcgaa gtggatgagt atccggaagc
     1321 agcgttcaac ctcaagggtt ctattgacga tgtgatcgaa gccggtaaga aaatgttggc
     1381 ggaagccgaa taatcatttg ccggttttaa aaccattcag acaatgaaaa tttccatact
     1441 gactcctgag cgcgaggttt ttaacggcct tgtcacctct gtaaaagtgc cgggcacgcg
     1501 cggcgagttt cagatattga ataaccacgc acccattgta tcttctctcg atgcaggctt
     1561 catccatgtg gtgacggccc agggcgagta ccgctatttt gacgatgaaa gcggtgaaat
     1621 caaaactgcc gcagagccgg ggcgcagcct gcggttcaaa attgccggcg gttttgtgga
     1681 agtacttcgc gatgaagtgt cgctgctggt gcatggcgta agagaataaa ttgacttggc
     1741 acacaagtca ttttgctgag tttacaggct gaagctatcc gtagtttcag cctttttttt
     1801 gccccaactg agtactctca ctgtccgcgc tgccaaaaat catttgcatt ccccgatcat
     1861 ataccgtaac ttgagcaatg aaaaccattg ttgccaaccg ttatgaaaaa aatactcttt
     1921 cccaccgatt gctccgcttc tgcgcaccac gcattccggt ttgccgtaca attggcccgc
     1981 caattggatg ccgtcatcga catcatgtcc gtgtatcatt taccggccac cgacgcaagc
     2041 agtgttccgc cttcgtacat tgaaacaatg atcgaagaac gccgccgaca agtggaagcc
     2101 aatgtagcca agtttctgga ggcctggccc gatgcgccca ttggacaacg ctttgccgac
     2161 tacggcattt ttacccatca ggaaatagtg gacaaagcga cagagggtag ccatgagctg
     2221 attgtgatgg gtacgcacgg cgttggtaat gctgtggaca aactcctcgg cagcgtcact
     2281 acgcatacca tcatgcaagc cccctgtccg gtgctggcca ttccgccggg ggccgagtgg
     2341 aaacccatcc ggcacattgc ttatgctacc gactttgagg caggcgacgg acatgccgtg
     2401 tacaacctca tggagtttgc caaattgatg ggcgctcagg tgcattttgt acacatacat
     2461 accgacgata aagagatcgg catggaagaa ttggaccgcc cggaagcaca tcccgaagca
     2521 tttgccgact ttaccatcgt acacaatcct tccgtcatgg acggcctcga ccaatatgtc
     2581 agggaacgct ccatagacat ggtggcgctg tttataccgc gccggagaat ttgggaacgc
     2641 ctgtttcaca gcagcttctc caaaaaaatg gctttccata cggcattacc tttgctcacg
     2701 ttcagagcat aaagtggcca acgctggatg gagccgtcaa cgaatccggg ggaaccccga
     2761 aaccctctgt ggcgcagcgc tcgctggtcg gccctgatta ccgtgttcac cgccggcgga
     2821 agtttgttgt ggatgctcgt cctcacgcgg ctgctgtcgc cggctgattt ttctacttcc
     2881 gcgctggctt tggccctcgt cggcgtggta cttttgtttt tgcaaaacgg tctgcccggc
     2941 gccatcattt ttcaacagga acgcagaagc ggcgtgcttt caacgctgtt ttggagcaat
     3001 ctcgcgctgg gattggccgg cactttgctc ctggctgtat cctctttcgg agcagggatt
     3061 ttcttcgaag acctccggtt gcatcaggta ttgttgacca tttcgccact gcccttgttg
     3121 tcggcgggcg gggccgttta caaaagcata catctgcgcg atttgaacta tcgcacggtg
     3181 gcggtggtag aactgacggc ctttacggcg ggagtggccg cttcggcatg gctggttttt
     3241 tccgggcatg gctactgggc cgctgtggcg caatgggcgc tgcgatatgc tgcggaggcg
     3301 gtattgtatg gtatggccgg tcttgggctt ttttcaccta tgcccccttc ccgaaacttc
     3361 caaaatctgc gaacgcacca gcaatttgct gccgggcagt gggccgagcg cctcaccatg
     3421 tacctcaatg ccaacataga tacattgttg attgcaaagc tcttaggcgc cgaagccctg
     3481 ggcgtgtatg atgtgttcaa acgcctcatt gcgcggccta cttcaatggc gggcgagtgg
     3541 ctcgaccgtt ttgcttttcc gctaatggcc cggagcgaaa caccggcgcg catttttttt
     3601 accaataccc gcctgctcag tatcctgctg gcgccgatgg ccgtgttggg cattctgctg
     3661 gccgggccgc tgcttcaggt gctgaccgga gcggcatggc aggtcgaatt gcgcacgttc
     3721 caatggcttg tggccgccat agctgtggcc gcgctggcgc atccctggga cggcctgctg
     3781 gcggcaaccg ggcggctaaa gcaactgagt ttggccaata tggcttatgc catgctcctt
     3841 tcggcgggat tgtgggctgg tgcggtctgg gggctggaag gcgcggcggc agcgcaggct
     3901 gtattggccc cgctgtggat cgtggttgga tattcctggc ttattgagcc ggtgaccggg
     3961 caaggcatgg tagttttttt gcgccctccg gcaaagtgga tcggcgctgc ccttctttgt
     4021 gccgcgccgg catgggcatg gctgcaaata caaaacacgc ctgccccggt accgaccttg
     4081 atattggcag tattgagttt tacagcctgt tatggctggg cggtgtggaa gtgggggctg
     4141 cgctaaattt tacccgtaca cctccctttt cccagccttg agcgtattga gcatcaacga
     4201 cacaacggtc atctggccta cgccgccggg tacgggcgtg aagtaagacg acttgggcgc
     4261 taccgaagcg aaatccacgt cgccggtgag gcgatagccg tttttggcgg cggggtcttc
     4321 cacgcgattc atgcccacat caatgatgac aaccccgtct ttgaccatgt cgcctttgat
     4381 gaagtcggcg ctgccgatgg cggccacgat gatgtcggcc cggcgggtgt gttcggcgag
     4441 gttgcgggtg cggctgtggc agatggtgac ggtggcatcg ccgggataag ctttgcggct
     4501 gaggaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncact acttctttgc
     4561 cggcggtggg aatctcgtag cgcttcagca tttcgaggat accggcgggc gtagcgggca
     4621 ggtagcaagg cagcccttga gccatgcggc cgaaattgac cggatggaag ccgtccacgt
     4681 ctttgcgggg atcaatggct aaggtgattt cttcttcatt gaggtgtttg ggcaggggaa
     4741 gctgcacgat gaagccgtca atgtcggggt tttggttgag gtcggccacg atacccatca
     4801 gttcctcctg cgagatgtcg gcagggcggc gaatgagggt agagtgaaag cccacttctt
     4861 cgcaggattt cactttgttg cgcacataca cctggctggc ggggtcttcg ccgatgagta
     4921 cggccgctag atgaggcgct ttgccgcctg ctgcaacg
//
LOCUS       DAORLF010000370         4938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9362_length_4938_cov_2.153185, whole genome shotgun sequence.
ACCESSION   DAORLF010000370 DAORLF010000000
VERSION     DAORLF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9362_length_4938_cov_2.153185"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..465)
                     /locus_tag="PK198_21670"
     CDS             complement(94..465)
                     /locus_tag="PK198_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF41423.1"
                     /translation="MRDIFLDISLNIRTVDSSIISITITDREGATNVYEAPTDMNMNL
                     MEVCKAHELPVKGTCGGMALCASCHVYVLTDHELPEMSGDEEAQLDQAFFVKNNSRLG
                     CQLRITPEMDGLAVQLAPESE"
     gene            complement(545..1951)
                     /locus_tag="PK198_21675"
     CDS             complement(545..1951)
                     /locus_tag="PK198_21675"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF41424.1"
                     /translation="MKKLLTAAVLFLLYACAVQHDTPLPALTTRPDWKTLEVPATPQD
                     LSGNPEAGLRFLLYGNYLGTGVPWSMVEKRFTRTVRDTLWQRSGPNAHAPYLMNVFEA
                     ANGVPVMNGNCFTCHAGALNGRLVPGLGNSFSDYRRSFKPMANATSMAVRIKYGKRSA
                     EWEAFEDFGRYFKKMAPHIRTGQPGANPAFRLAEACMMHRNPDDLSFRETPQFDISPF
                     PVATDVPPLWNVKKKNALYYDGIGRGDFSKLIFQASVLGIPDSTAARQAIAGFKDVLA
                     WLHTLEPPAFPEKINEQKAARGQLIFEDKCAKCHGAYGAQPSYPNKVVALEVIKTDPV
                     YVQYIHQSGITAWYNSGWFAQSEPRSWFEPLPGYVAPPLDGIWATAPYLHNGSVPTLE
                     ALLHSPSRPVRWQRSGDSRDYDLEKVGWKYTVPTRAQKKGWTYDTTLPGYGNGGHTFG
                     DALSADDRAALIEYLKTL"
     gene            2773..3207
                     /locus_tag="PK198_21680"
     CDS             2773..3207
                     /locus_tag="PK198_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41425.1"
                     /translation="MRPLTHQNNSAPKGVLDTNSTNYDDCFTTEHPARAEIPFTKPET
                     VWRRTFQHNMPFCLYSLYQLQPMFSAHSTSSGHMSNLQLELLKLYATNIPEQQLLDIK
                     QLLAAYFAELIDRDMSALWEEKEWDERTIEIWKNEHLRTSYK"
     gene            3216..3644
                     /locus_tag="PK198_21685"
     CDS             3216..3644
                     /locus_tag="PK198_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF41426.1"
                     /translation="MRIVLDINVLLISLPVRSPYRPIFDAVRLGDLTLLITNDILMEY
                     EEKIAEKTRPEIAQNVVRLLLNLPNVEKIDAWYRWNLIGADADDNKYTDCAIAANAKY
                     IVSDDAHFRVLKRLSFPKIDLLTSDELLEIVRNELPPRSR"
     gene            3849..>4938
                     /locus_tag="PK198_21690"
     CDS             3849..>4938
                     /locus_tag="PK198_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF41427.1"
                     /translation="MTIASPQNTFRSIPGDPFQVLEHTLSNGLRIFMSVNPFEPRIFT
                     NIAVRAGSKQDPADTTGLAHYMEHMMFKGSSRIGALDWEKEEQLLQRISDLYEQHRAA
                     ETEEERLAIYAEIDRVSNEAAPLAAANEYDKLSGAIGANHTNAYTWLDQTVYVNEIPC
                     NELERWMRLESERFRMMALRLFHTELETVYEEFNISQDRDFRKVGHALRAAIYPTHPY
                     GTQTTLGSPEHLRRPSQVNIQRYFHTYYVPNNMAILLSGDFDPAEVVELAERYFGNYE
                     ARPVPPFTFEEQPPITAPIRREVFGQEGPYLQMAWRFGPAASEEQVMLSMVRRILYND
                     QAGLLDTQLNQTQMVLESEAWSWPNADYT"
BASE COUNT         1131 a         1401 c         1318 g         1088 t
ORIGIN      
        1 tcctcgcgct tagtaccgct taccgcccac tgcccaccgc ctactggttt ccctcgcgct
       61 tagcaccgcc caccgcccac cgcctactgg atctcactcc gactccggcg ccaactgcac
      121 tgccaggccg tccatttccg gcgtgatgcg aagttgacag ccgaggcggg aattgttttt
      181 tacaaaaaaa gcctgatcca actgagcctc ctcgtcgccg gacatctccg gcagttcgtg
      241 gtctgtcagc acatacacat ggcaggaagc gcacaaggcc atgccgccgc aggtgccttt
      301 tacggggagt tcgtgcgcct tgcacacctc catgaggttc atgttcatat cggtaggcgc
      361 ttcataaaca ttggttgcgc cttctcggtc ggtgatggtg atggaaataa tacttgaatc
      421 cacagttctg atattcagtg agatgtctaa gaaaatgtcg cgcaaaaatg ccgcttcttt
      481 tgcctggcta ttgtgacccg gcgcacagaa ggggatgtca aaagtttgct gaaagaccgc
      541 aaacttacaa agttttgaga tactcgatga gtgcggcgcg gtcgtcggcg ctgagcgcat
      601 ctccgaaagt gtggcctccg ttgccatagc cgggtagcgt ggtatcgtaa gtccagcctt
      661 tcttttgggc gcgggtaggc acggtatatt tccaacccac tttttcgagg tcgtaatccc
      721 ggctgtcgcc gctgcgctgc cagcgaacgg ggcgcgaagg gctgtgcagc aaggcttcga
      781 gcgtaggcac cgagccgttg tgcaggtaag gcgcggtggc ccagattccg tccaaaggcg
      841 gcgcaacgta gcccggcagc ggttcgaacc aggagcgcgg ctcgctttgg gcaaaccagc
      901 cggaattgta ccatgccgtg atgcccgact gatggatgta ctgcacatac acggggtcgg
      961 ttttgatcac ctctaaggcc accactttgt tggggtacga tggctgcgcg ccataagcgc
     1021 cgtgacattt ggcgcatttg tcttcaaaaa tgagttggcc gcgtgccgct ttttgctcat
     1081 ttattttttc agggaatgcc ggcggttcca gcgtatgcag ccaggccagc acgtctttga
     1141 accccgcaat ggcctgtcgg gcggcggtgc tgtcggggat gcccagcaca gaagcctgga
     1201 agatgagttt ggaaaagtcg ccccgcccga tgccgtcgta atacagggcg tttttctttt
     1261 tcacattcca cagcggcggc acatcggtgg ccaccggaaa cggcgatatg tcgaattgcg
     1321 gcgtctcgcg gaaactcaga tcatcgggat ttcggtgcat catgcaggct tcggcgaggc
     1381 ggaaagccgg gttggcgccg ggctgtccgg tgcgaatgtg cggggccatt tttttgaaat
     1441 aacggccaaa atcctcaaac gcctcccact ctgccgagcg cttgccgtac ttgatccgga
     1501 cggccatcga tgtcgcattc gccatgggtt tgaaactgcg ccggtagtcg gaaaaactat
     1561 tgccgagacc cggcacgagc ctgccgttga gcgcgccggc gtggcaggtg aagcaattgc
     1621 cgttcatcac cggtacgccg ttggcggctt caaacacatt catcaggtag ggcgcgtggg
     1681 cattggggcc gctgcgttgc cagagggtgt cgcgtacggt gcgggtgaag cgtttttcca
     1741 ccatgctcca gggtacgccg gtgccgaggt agttgccgta cagcagaaaa cgcagtccgg
     1801 cctccggatt gccgctcagg tcttgcggcg tggcgggcac ttcgagggtt ttccagtcag
     1861 ggcgggtggt cagggccggc agcggggtgt cgtgttgcac ggcgcaggcg tacagcaaaa
     1921 agaggacggc ggcagtgagg agttttttca tggtaatggt tgaggaaaat aaggatgcgg
     1981 gtagaacccg ccggatagtc cgcacgaggt atgaacctcg cgcgggcagc gatgatcgta
     2041 acaattgagc ttggattggg ttgagaggcg agcgttttca ggtgacaaac tgagcgtcaa
     2101 gagttagcga ggcgggaagg tgtcgcatga aaacgggggg agatttaatt ctcaaatttg
     2161 cttctttcag cgtcgaagac aggccgtaac ttcgggtacg ccctgacttg tgaggcaaaa
     2221 tccgaaatcc gcccgttcct gccggaacgc aaaaatcttt aacgcgaatt ttagcgaagg
     2281 gcacgaattt ccgcgaacga gaggggcata acgatcacaa gaatctgcgt ctttcagcgt
     2341 cagaaatcta cgcctctttt tcaggcatga ttcacaggat ggctctacgg accaatcggc
     2401 cataaaaaca acgaaatacc cttccatctt ccccaacttt tcgcaccttt gcacgaaaac
     2461 caacggcatg tcggaaacgg cccaaaatcc actcaacacc ttcatcatct acgcccgcga
     2521 ggacaaagac gcgctgctgg agttgaaaaa gcagctcatc cccttggagc gcagccggca
     2581 gattgccctg tggtacgacg gggagatcgt gccgggcgag gtaaaggagg tttgcctttc
     2641 ctggcctaac tatgagcaac cgctcgcgcg agattccatc tcgtgcggcc ttattatgcg
     2701 ggttccacct aagtttacca cgcagggcgt ggcgtataaa aaactcgttc aactcccaaa
     2761 tttatcgcac ctttgcgccc gctcactcat caaaacaatt cggcgcccaa gggcgtactc
     2821 gatacaaact cgaccaacta tgacgattgc ttcaccacag aacaccccgc tcgcgccgag
     2881 attccattca ctaagcctga aaccgtgtgg cggcgaactt ttcaacataa tatgccattt
     2941 tgtttgtatt cattatatca acttcagcct atgttcagcg cacacagcac ctcaagcgga
     3001 cacatgagta accttcaatt ggaattgctc aaactttatg ccaccaacat cccggaacag
     3061 cagttgctgg acattaaaca actgctcgct gcttatttcg ccgagttgat tgacagggat
     3121 atgtctgcgc tttgggaaga aaaagaatgg gatgagagga cgattgaaat ctggaaaaac
     3181 gagcatttgc gtacctcata caagtaagcg ttcgtatgcg aatagtatta gatatcaacg
     3241 tattactgat ctccctcccg gttcgttctc cataccgccc aatatttgat gccgtcaggc
     3301 tgggtgattt gactcttttg attacgaacg acatactcat ggagtacgag gaaaagatag
     3361 ccgaaaagac ccgaccggaa attgcacaaa atgtcgtccg tctgcttctg aacctcccca
     3421 atgttgaaaa aatagatgca tggtatcgct ggaatctgat aggtgcggat gctgacgaca
     3481 ataaatacac cgactgtgct attgccgcca atgccaaata catcgtttcg gacgatgctc
     3541 attttcgagt attaaagcgc ctttctttcc cgaagataga tttgcttacc tctgatgaat
     3601 tgctggaaat agtaagaaat gaactccccc cgcgctcgcg ttgagattcc atcttcatgt
     3661 ttcccgcccc gctcgcgcga gattccatct cgtgcggcct attatgcggg ttccacctaa
     3721 gtttaccacg cagggcgtgg cgtataaaaa actcgttcaa ctcccaaatt tatcgcacct
     3781 ttgcgcccgc tcactcatca aaacaattcg gcgcttcagg gcgtactccg atacaaactc
     3841 gaccaactat gacgattgct tcaccacaga acaccttccg ctccattccc ggcgacccgt
     3901 ttcaggtgct ggaacacacg ctctccaacg gcctccgcat ctttatgagc gtcaatccct
     3961 ttgagccgcg catcttcacc aacatcgccg tgcgcgccgg ttccaaacaa gaccccgccg
     4021 ataccaccgg tctggcgcac tacatggagc acatgatgtt caaaggcagc agtcgcatcg
     4081 gcgctttgga ctgggaaaag gaagagcaac tccttcagcg catttccgac ctttacgaac
     4141 aacatcgcgc cgccgaaaca gaggaagaac gcctcgccat ctatgccgaa atagaccgcg
     4201 tgtcgaacga agcggccccc ctggctgccg ccaatgaata cgacaaactc tccggagcca
     4261 tcggcgccaa ccacaccaat gcctacacct ggttagacca gaccgtatat gtcaacgaaa
     4321 taccctgcaa cgagttggaa cgctggatgc gcctcgaatc ggagcgcttc cgcatgatgg
     4381 ccctgcgcct gtttcacacc gaattggaga ctgtttacga ggagttcaac atcagtcagg
     4441 accgcgattt ccgcaaggtg ggacacgcgc ttcgggcggc gatctacccc actcacccct
     4501 atggcaccca aaccacgctg ggcagtcccg aacacctccg gaggccttcg caggtcaaca
     4561 tacagcgcta cttccacacc tattatgtgc ccaacaacat ggccattttg ctgtccggcg
     4621 atttcgatcc ggcagaggta gtggaattgg ccgagcgcta tttcggcaac tacgaagcgc
     4681 gccccgtacc gccttttact tttgaagaac aaccgcccat cacggcgccc attcgccgcg
     4741 aggtgttcgg gcaggaaggg ccgtacctcc agatggcctg gcgctttggt cccgctgcat
     4801 cggaggaaca ggtgatgctc tccatggtac gccggatttt gtacaatgac caggccggcc
     4861 tgctcgatac gcaactcaat cagactcaga tggtgctgga atcggaggcc tggagctggc
     4921 ccaacgccga ctacaccg
//
LOCUS       DAORLF010000371         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9415_length_4918_cov_1.566523, whole genome shotgun sequence.
ACCESSION   DAORLF010000371 DAORLF010000000
VERSION     DAORLF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_4918_cov_1.566523"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1171
                     /locus_tag="PK198_21695"
     CDS             46..>1171
                     /locus_tag="PK198_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41428.1"
                     /translation="MKTTTQHNTTQHNTPDKFSSWTILMYMCTLFFCLSGHHSLQAQN
                     PFECHTGSVMNGEGINIQGNTPIAPCWPISTQPCKNTLSYAPDPDFPEHTPIRYVRIV
                     LHIFQNSSAPVLPPGNFTNSLEHINVLKSWFHHPTAGLNARMANLCDPQPKVPGFETP
                     FISDSRLRFIFEGTENKDIFFYNHPGYWAAGHYCGLNLHNSMYNFAVTNNSIVNSDPD
                     LLNAVHVFLTGASACGSSYNYGGGGYTIMNRSCGRTSNQLGIVLYGQFYEYIYDLTGQ
                     PPVPNFTDIPPNQIDIPYGGNGILSEFLHMASIDHPCCNSHLSGGDQCNDTPHNMQTS
                     NNVQQTFYQGSLLHESLRCAFTQCQLGRLHHFFEHLQPDWQ"
     assembly_gap    1172..1346
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1347..>2867
                     /locus_tag="PK198_21700"
     CDS             <1347..>2867
                     /locus_tag="PK198_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41429.1"
                     /translation="KDARITVHPNGELYVDGARLHNNCDNARWEGIIVAGNSSLHQYK
                     TYGVRNQGLLILSNGATVSGTNTAVRNYVPENPAYQSGGVIMADKATFKNNLIGVDFA
                     PYQNHHFLYGSLQGDLSYFSNCSFLADAQFGADYGAFYSMASMRGVTGIEFQACTFAN
                     DYPNASLSYADEKQSGIRATNAGFEVTGICQTNPLEPCTAYLPSEFRGFAIAISAGNY
                     GSIKTFSVSNTNFNNNARGIVAQRVDNIYAVNNAFKIGAALPHKNGVPFMGIDMYRCT
                     GYKVEENAFTTIAAPHSQTPAVGVLAHNSGSDPNTVYKNTYKGLSYANLSNGNNRGNL
                     LNNGGLQYICNGNEANMFDIAVPAENVSSTGISMFQGSASASAGNIFSVAQANSPAEM
                     HILNHAAPIIYFEPPAPTNYSFGSVTVVTGNANVCASKLPGRIEKGTLSHLERQSFET
                     NFWNETDVWAKSEMAGALIRDRLLQEDSIQLGAARTWLSHKGTLDARFAVVDSWIQA"
     assembly_gap    2868..3076
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3077..>3313
                     /locus_tag="PK198_21705"
     CDS             <3077..>3313
                     /locus_tag="PK198_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41430.1"
                     /translation="QAQVLLNEVAGAGYRPQVILPTWGSQNISIPSVGSVPPPLEEEA
                     PLRIQAVPNPATQETVFHYSLPFGAERAQIIVRS"
     assembly_gap    3314..3447
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3691..3700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3701..>4013
                     /locus_tag="PK198_21710"
     CDS             <3701..>4013
                     /locus_tag="PK198_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41431.1"
                     /translation="RFSLGFPVSVATNVIVKDTCYYFSGLVIDSVSPHRISNIFMKTD
                     LNGNPVNVKLLRSPFRSYQTWNPDFAELSDGSFAISAYSYDSISRAMLIRYAPSGDTL
                     FT"
     assembly_gap    4014..4086
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4087..>4430
                     /locus_tag="PK198_21715"
     CDS             <4087..>4430
                     /locus_tag="PK198_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41432.1"
                     /translation="CPDGGYLFACDIQGLLWNGYANVDILIVKTDSLGDIQWQKKLGN
                     HWAERPYSLIFDGDGGIIVGGQKANFNLTVHNYTFQTSITKLDSLGNIQWDYLSPLSE
                     GLRFGAADMVLL"
     assembly_gap    4431..4471
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4492..>4918
                     /locus_tag="PK198_21720"
     CDS             4492..>4918
                     /locus_tag="PK198_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41433.1"
                     /translation="MNTVFFDKYIFKLNPQREVEWEISFPEPVLTSQSKLSNLIALSD
                     GSGFVAAGSHGEIFSGQNASLKGWIGKISPQGDSIWTRQYLGVQNNNNLHYIHDIKET
                     ADGGFILCGESQNLNNAPGDPIQQAWLLKLDAYGCLVPGC"
BASE COUNT         1117 a         1116 c          967 g         1076 t
ORIGIN      
        1 gcatttcttc acccgcttcc gaaaggaggc ataaatcgtt gttatatgaa aactacaaca
       61 caacacaaca caacacaaca caacacccct gataagttca gctcttggac aatcctgatg
      121 tatatgtgta ccctcttttt ctgcctttcg ggacaccatt ctttacaggc gcaaaacccc
      181 tttgagtgtc atacgggaag tgtaatgaat ggagaaggaa tcaacattca gggaaatacg
      241 ccgattgctc cttgttggcc aatatctact cagccatgta aaaacaccct gagctatgct
      301 cctgatcctg actttcctga acatacgccc ataagatatg taaggatagt gctgcatata
      361 ttccagaatt cgtctgctcc ggtattgccg ccgggaaatt tcaccaattc cctggaacat
      421 attaatgtat tgaagtcttg gtttcatcat cctaccgcag gattgaatgc cagaatggcc
      481 aacctgtgcg accctcagcc caaagtaccc ggctttgaaa cgcctttcat ctctgattca
      541 agactgcgat tcatttttga gggaacagag aataaagata ttttctttta caatcaccct
      601 gggtattggg cagccgggca ttattgtgga ttaaatcttc acaattctat gtataatttt
      661 gccgtcacca acaacagtat tgtcaattcc gacccggatt tattgaatgc agtgcatgtt
      721 tttttgaccg gagcaagtgc ttgcggctcc tcgtataatt atggcggtgg tggttataca
      781 ataatgaacc ggtcttgcgg aagaacaagc aatcaattag gcatagtgct atatggccaa
      841 ttttacgaat acatatatga cctcactggt cagcccccgg tgccgaattt tactgatata
      901 cctcccaacc agattgatat tccctacggc ggcaacggca ttttgtcgga gtttctacac
      961 atggcgagta tagaccatcc ttgttgcaat tctcacctat ccgggggcga tcagtgcaac
     1021 gacacgccac ataatatgca aacgtcgaac aatgtgcaac aaacctttta tcaaggcagc
     1081 ttactccacg aatccctccg ctgcgcattc acgcagtgcc aattaggacg actgcatcat
     1141 ttttttgagc atttgcaacc agactggcag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa gatgcccgaa taaccgtgca tcccaacgga
     1381 gaattgtatg tggacggcgc gcgcctgcac aacaactgcg ataatgcccg ctgggaagga
     1441 atcatcgttg ccggcaacag cagtctgcac cagtacaaaa cttacggagt cagaaatcag
     1501 ggcttgctca tcctgtcgaa tggtgcaacg gtatcaggga ccaatacggc ggtacgcaat
     1561 tatgtgccgg aaaacccggc ataccaatcg ggcggggtca tcatggccga taaggctaca
     1621 ttcaaaaaca atctcatcgg cgtggacttt gcgccctacc aaaatcatca ctttctgtac
     1681 ggcagcctgc aaggcgatct aagttatttt tccaattgca gctttttggc cgatgcacag
     1741 ttcggcgccg actacggggc attttacagc atggcgagca tgcggggcgt aacgggcatt
     1801 gagtttcagg cctgtacttt tgccaatgat taccccaatg cctctttgag ctatgccgat
     1861 gaaaaacaat ccggcattcg ggcaacaaat gccgggttcg aagtgacggg catttgccag
     1921 accaatccgc tggaaccctg cactgcttac ctgccctccg aattcagggg ctttgccata
     1981 gccatatctg ccggaaacta tgggagcatc aaaacctttt ctgtgtcgaa cacgaatttc
     2041 aacaacaatg cgcggggcat agtggcccaa agggttgaca atatatacgc cgtgaataat
     2101 gcctttaaaa taggcgccgc gctgccgcac aaaaacggcg ttccgtttat gggaatagat
     2161 atgtaccgct gcacaggata caaagtagaa gaaaatgcct tcactaccat tgccgccccc
     2221 cattcccaaa cgcccgcagt aggcgtactc gctcacaatt ccggcagcga ccccaatact
     2281 gtttataaga atacttacaa gggcttgagc tatgccaatt tgtccaacgg gaataataga
     2341 gggaatttgt tgaataatgg tggtctccaa tacatttgca acggcaacga ggccaatatg
     2401 ttcgacatcg ccgttcctgc agaaaatgta tcttctacgg gcatatctat gtttcagggt
     2461 tctgcgagtg caagcgccgg aaacatcttc tccgtggcgc aggccaactc tcccgctgaa
     2521 atgcacatcc tcaatcacgc cgcgccaatc atatacttcg agccgcctgc tcctacaaat
     2581 tattcgtttg gttccgtaac agtagttacc ggcaatgcaa atgtttgtgc gtcgaaactg
     2641 ccgggccgta ttgaaaaagg tacgctcagc catctggaaa gacagtcttt tgaaacaaac
     2701 ttctggaacg agacggatgt ctgggccaaa agcgaaatgg ccggcgccct catcagagac
     2761 cgtctgctac aggaagacag catccaactg ggggctgccc gcacctggct gtcgcacaaa
     2821 ggcacattgg acgcccggtt tgcagtggtt gattcctgga tacaggcnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnngcag gcgcaggtgc tgctcaatga ggtggccggg gcaggctacc
     3121 gaccgcaggt aatactgccg acatggggca gccaaaacat ttcgattcct tccgtaggca
     3181 gcgttccgcc gccgctggaa gaagaagccc ccctgcgcat acaggccgta cccaatccgg
     3241 ctactcagga aacggtgttc cactacagcc ttccattcgg cgctgagcga gcacagatca
     3301 tcgtccgctc catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnntgg tcatcattcg ataacgcgtg taacttgatg
     3481 atggctgccg gcgaacagga cacaacaacg caggcttttc ccttcgtttt gtcttcattt
     3541 tgccggcagc cgttttccct gctcctttgc cgtatttccc ggtatctccc tcacctccca
     3601 agcattgcat ccatgaaaaa aatatctcat ttcattttcc tgttgtgtct gcctactgcg
     3661 ttcctttttt cacagcccac tttcaacaag nnnnnnnnnn cggttttcac tgggctttcc
     3721 tgtcagtgta gccaccaatg tgattgttaa agacacctgc tattatttct caggtttagt
     3781 catagacagc gtatctcctc acagaataag caatattttt atgaaaaccg acttgaacgg
     3841 caatcctgtg aatgttaaat tgttgcgaag cccgttccgc tcttatcaga cctggaatcc
     3901 cgattttgca gaactttctg acggcagttt tgccatttca gcctattctt atgactctat
     3961 atccagagct atgctgatca gatacgcccc ctccggcgat actttattca cccnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnntgcc ccgacggggg atatttattt gcatgcgata tacagggact tctctggaac
     4141 ggatacgcca atgtggacat cctgatcgta aaaacagata gtctggggga catacaatgg
     4201 caaaaaaaat tgggcaatca ttgggctgaa aggccctatt ctttgatatt tgacggtgac
     4261 ggcggcatta tcgtaggcgg gcaaaaggcc aatttcaacc tcaccgtcca caactacaca
     4321 ttccaaacga gcattacaaa attggacagc ctcggcaata tacaatggga ctacctctct
     4381 ccgctttcag agggactgcg cttcggcgcg gccgatatgg tcttgctgga nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgaactct atcgcccctc agtgaatact
     4501 gtttttttcg acaagtatat tttcaagctc aacccacaaa gagaagtaga atgggaaatt
     4561 tcctttcctg agccggtgtt aacgagccaa tctaagctct ctaatctcat tgctctttca
     4621 gacggcagcg gatttgtggc ggcaggctcg cacggggaga tattttcagg tcaaaatgcc
     4681 tccctaaagg ggtggatagg aaaaatttct ccacaaggcg atagcatttg gacaaggcag
     4741 tatcttggtg tacaaaataa taacaatctt cattacatac acgacatcaa agaaacagcc
     4801 gacggaggtt tcatcttgtg cggggagtct caaaatctca acaatgcccc cggagacccc
     4861 attcagcaag cctggctgct caagttggac gcttacggct gcttggtgcc gggctgcc
//
LOCUS       DAORLF010000372         4913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9428_length_4913_cov_1.586044, whole genome shotgun sequence.
ACCESSION   DAORLF010000372 DAORLF010000000
VERSION     DAORLF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9428_length_4913_cov_1.586044"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK198_21725"
     CDS             complement(<1..638)
                     /locus_tag="PK198_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41434.1"
                     /translation="MKCWGYLAIMVLAIQALSACSKQQAEKTGDVPLARVFNKTLYLS
                     DMEGMFPPDADSALIINSYIERWIREALLLHEAERNIPSDLNIDKLVRDYRASLVRHS
                     YEQFLVEQLLDSTVTQQELQAFYENNKEQYQLETPIMRCFFIKMPRPVYQIDELRRLW
                     NSERPEDYAGLVKYCSEFAESHLLEDSTWHKVEDIAAELPAGLLSADHPGGKR"
     gene            complement(650..>2111)
                     /locus_tag="PK198_21730"
     CDS             complement(650..>2111)
                     /locus_tag="PK198_21730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF41435.1"
                     /translation="HSDDKSALNNGGHIGFITAIFPPGMYALETAAYTSKLNQLTGPV
                     RSSGGYHLMKIGARRPARGEMEVAHILIRKAEGQEQQAKQRIDSVYQAMGGATFEEMA
                     ALSDDRNSGPRGGYLGFFGINRYEPAFENAAFGIAKDGDYSRPFESAAGWHIVKRISK
                     RDIQPYAVEKPRLENRIKNDERFEEARRAMVENIRKQNNFKENLQTLEQYAAGLTEEF
                     LTFKWKSNPEKSPAVLFTLGKTDKATIADFEEFLTNSSRKRLRFSGSMEPAAAAKLLY
                     KDFVDDYCLRFEEEHLYEKYPDFRALMREYEEGILLFEATKMQVWDKASQDTVGLEQF
                     YNKYAKGKYRWDERAVIHKWQIAPEAKDQVGDILKTVSKTSAEAALAKFNTPEKQILT
                     LETSTYERTKAPAGITDEKWRVGGMSAAVQDPKTQVHTFYRIESITPPSDKTLKEARG
                     YIVADYQDYLEAQWVEQLRKEYKVDINRSVFDKLIK"
     assembly_gap    2112..2204
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2205..2618)
                     /locus_tag="PK198_21735"
     CDS             complement(<2205..2618)
                     /locus_tag="PK198_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF41436.1"
                     /translation="MMAQLKTVFALLLFLPGLLAAQQNDPVLFTVHKTPVHASEFSYI
                     YAKTNGKNADFSRKSLEEYLDLYVKFKLKVQKARDLHIDTIPSLKSELDGYRRQLADS
                     YLVDKEVTEKLTKEAYERIQQDVDLYHIFFAVPANA"
     assembly_gap    2849..2913
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2914..3881)
                     /locus_tag="PK198_21740"
     CDS             complement(<2914..3881)
                     /locus_tag="PK198_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41437.1"
                     /translation="MTHTIDVDKQELLLVGNSTIPKKERDNPRYKTLIHRYNSELNLI
                     SSNSFELDHPHTVLFSGADYIDGKKFLMVLAPVSATKSSATVPNPDQYKLVIINEKGE
                     LERIIDFFTLCHRWKINGISVIEDEIIIYGIGEELVDKLGAADQKRRKSYIDGWATFV
                     EPAAYIQIITLKKDGPEVKAQTIPVADFQLTAQSIDNSPVSPLILNGKFDLIGLQKSK
                     NGRILLSGTAYIPRLYPRAYTDYFFVHIDSTGNLSQLFCTKNPEPGSGFFLKTAQTYL
                     GDSYIYESPNDPDRLLWMLRYAAKTGLSLGGVSVRNTVKYTPVMYE"
     assembly_gap    3899..4051
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4052..4368)
                     /locus_tag="PK198_21745"
     CDS             complement(<4052..4368)
                     /locus_tag="PK198_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41438.1"
                     /translation="MKNYLVAGLCAISLSFSPPLFSQCLWRETVINFDKEEENKKNFL
                     GVISYKDSEYTDLVFIKKKPKDEVVYETIRLDKELYTVEKFEEADKDFGKDVFKGISY
                     VTT"
     gene            complement(4515..>4913)
                     /locus_tag="PK198_21750"
     CDS             complement(4515..>4913)
                     /locus_tag="PK198_21750"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRF41439.1"
                     /translation="QFRVAVNEQWQEGVAGSIRLAVMEALAVRPDLENLLFLLSDQPF
                     VTTQILSELLDLQSRTNSSIAACRYGDVLGVPAVFSKKWFPELLLLQGDSGAGRLIRQ
                     YAGEAAVLPFEPGAVDVDTEEDYLLLCSWS"
BASE COUNT         1048 a         1147 c         1171 g         1236 t
ORIGIN      
        1 cgtttaccgc cgggatgatc ggcagacagt agtccggcgg gcaattcggc ggcaatgtct
       61 tccaccttgt gccaggtact gtcttccagc aggtgcgatt ctgcaaactc cgagcagtat
      121 tttaccaagc cggcgtaatc ttccggtcgt tcgctgttcc atagacggcg caattcgtca
      181 atttggtaaa ccggtcgggg catttttata aaaaaacacc gcataatggg cgtttcgagc
      241 tggtattgct ctttgttgtt ttcataaaaa gcctgtagtt cctgctgcgt aacggtggag
      301 tccaacagtt gctctaccaa aaactgctca tagctgtgcc gcaccaaaga agcccggtaa
      361 tcgcgcacga gcttgtcaat attgaggtcg ctggggatgt tgcgttctgc ctcgtgcagc
      421 agcagcgcct cccgaatcca gcgctcgatg tatgaattga tgatgagggc gctgtcggcg
      481 tccggcggga acatgccctc catatcggaa aggtagagcg ttttgttgaa cactctggcc
      541 aagggtacgt ccccggtctt ttccgcctgc tgcttgctgc aagcgctcag cgcctgtatg
      601 gccaacacca ttatcgccag gtatccccag catttcatat tcatcggcac tacttgatga
      661 gtttgtcaaa caccgagcgg ttgatgtcca ctttgtattc tttgcggagt tgctctaccc
      721 actgtgcctc cagatagtcc tgataatcag ctactatgta acctctggcc tccttcagcg
      781 ttttgtccga tggcggcgtg atgctctcga tgcggtaaaa ggtgtgaacc tgcgttttcg
      841 ggtcttgcac agccgccgac atgccgccta cacgccactt ctcgtcggtg atgccggcgg
      901 gcgctttggt gcgttcgtaa gtagaggttt ctaatgtaag tatttgtttt tcaggagtat
      961 taaacttggc caatgctgct tcggcagagg ttttggaaac cgttttcaaa atatcgccca
     1021 cctggtcttt ggcttcgggc gcaatctgcc atttgtgaat cacggcgcgc tcgtcccagc
     1081 ggtatttgcc tttggcatat ttgttgtaaa actgctccag cccgactgtg tcttgtgaag
     1141 ctttgtccca cacctgcatt ttggtggctt caaacagcag aatgccctcc tcgtactcgc
     1201 gcatgagcgc tctgaaatcg gggtactttt cgtacagatg ctcttcttcg aagcgcaggc
     1261 agtagtcgtc cacaaagtct ttgtacaaca attttgccgc tgctgccggc tccatagaac
     1321 cgctgaaacg caggcgcttg cgggaagagt tggtcaaaaa ttcctcgaaa tcagcaatgg
     1381 tggccttgtc ggttttgccc aatgtaaaca gcactgccgg cgacttttcc gggttggatt
     1441 tccacttgaa agtcagaaat tcttcggtca ggccggctgc atattgctcc agcgtttgca
     1501 ggttttcttt gaagttgttt tgcttgcgga tgttctccac catagcgcgg cgggcctcct
     1561 cgaagcgctc atcgtttttg atgcgatttt cgaggcgggg cttttccact gcgtagggct
     1621 gtatgtcgcg tttgctgatg cgcttgacga tgtgccagcc ggcagccgac tcgaatgggc
     1681 gggagtagtc gccgtctttg gcgatgccaa aagctgcgtt ttcaaaggcc ggctcgtagc
     1741 ggttgatgcc gaaaaagccg aggtagccgc cgcgcgggcc cgagttgcgg tcgtctgaga
     1801 gggccgccat ttcttcaaag gttgcgccgc ccatcgcctg atagacgctg tctatgcgtt
     1861 gcttggcttg ttgttcctgg ccttccgcct tgcgaatcag tatgtgagcc acctccattt
     1921 cgccgcgggc cgggcggcgg gcgcctattt tcatgaggtg gtagccgccg ctgctgcgaa
     1981 cggggccggt caactggttg agcttggaag tgtaagcagc agtttcgagc gcatacatgc
     2041 cgggaggaaa aatggccgtg atgaagccga tatggccgcc gttgttgagt gccgatttgt
     2101 cgtcggagtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcatt ggcaggcact
     2221 gcaaagaaaa tatggtacaa atccacatct tgctgaatgc gttcgtaggc ttctttggtg
     2281 agcttttcgg tcacttcctt gtctaccaga tacgagtcgg ccaactggcg acggtagcca
     2341 tccaattcgc ttttcaggct gggaatggtg tcaatgtgca ggtcgcgggc cttttgcacc
     2401 ttgagcttga acttgacgta caggtcaagg tactcttcga gcgacttgcg cgagaagtcg
     2461 gcgtttttgc cgttggtttt ggcatagatg tacgaaaact ccgaagcgtg taccggcgtc
     2521 ttgtgtacgg tgaacagaac cggatcgttc tgctgtgcag ccaacaggcc gggcaagaac
     2581 agcaataaag caaaaacagt cttcaattga gccatcatgg tgtagcattt ttttgaacgg
     2641 gcgcaaaaat aacatttttg aacgcaagag ggttggtttg tattttttaa acccctgcta
     2701 ccaagccctg cgacactcac cgcaccaatt tgccgaccca cacgctgttt tgctttttcc
     2761 tgcccagcag caaccattcg ccgggggtat tgagttcgac atgcggcagc gcaacattgt
     2821 cttgattggc aaaatcgccg aaaaggaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatttcgt acatgacggg ggtgtatttt
     2941 acggtatttc tgacggatac gcctcctaaa cttagtccgg ttttagctgc atatcgcagc
     3001 atccaaagca agcgatccgg gtcattggga gattcataaa tgtaagaatc gccgagatat
     3061 gtttgagcgg tcttgagaaa aaagccggaa ccaggctcgg ggtttttagt acaaaacaac
     3121 tggctcaggt ttcctgtaga gtcaatatga acaaagaaat agtcggtata tgcacgagga
     3181 tacaaacgag gaatgtaagc tgtacctgac aggagaattc gaccattttt acttttttga
     3241 agccctatca gatcgaattt gccatttaaa atgagcggcg ataccggaga gttgtctata
     3301 gactgagctg ttagttgaaa atctgctact ggaatagtct gggcttttac ctcggggccg
     3361 tcttttttca gggtgattat ctggatatag gccgccggct cgacaaaggt agcccaccca
     3421 tcaatatagc tttttctacg tttttgatca gcagcgccca gtttatctac taattcctct
     3481 cctattccat aaatgatgat ctcgtcttct atgaccgaaa tgccattgat tttccatctg
     3541 tggcagaggg taaaaaagtc aattattctt tcaagctctc ccttttcgtt gatgatgacc
     3601 aatttgtatt gatcgggatt aggcacggta gcagaagact tagttgccga taccggggcc
     3661 aaaaccatta aaaacttctt tccgtctatg taatcagctc ctgaaaaaag aaccgtatga
     3721 ggatggtcta actcaaaaga atttgaactg atcaagttca gttccgagtt gtacctgtga
     3781 atgagcgtct tgtatctggg attatctctt tccttttttg gtatggtgct atttcctacc
     3841 aataaaagct cctgcttgtc aacatcaata gtatgtgtca aatattgaaa cccgatttnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattgtggtt acatacgaaa tccctttaaa
     4081 aacatccttc ccaaaatcct tatccgcctc ctcaaatttt tctaccgtgt acagctcttt
     4141 atctaagcgg atggtctcat acacgacctc gtctttcggt ttctttttta taaaaacgag
     4201 gtcggtatat tccgagtcct tgtaagaaat gacacccaga aagttttttt tgttttcctc
     4261 ctctttgtcg aaattgatga ccgtttcccg ccaaaggcac tgggaaaaca agggcgggct
     4321 aaaactcaga ctgatggcgc acaggccggc aacaaggtag tttttcatgg cggtaagagt
     4381 tgtttttagt ttagtaaaaa tcaatgatcg gggcaggaaa cgatgcccgg cgacaaagat
     4441 aagcaaccgc gcctgaaatt ggcagcagat gctaattgct cccacaggtg gctccggtgg
     4501 ctggtattta gacctcaact ccacgaacac agcagcaggt aatcctcctc cgtgtccaca
     4561 tccaccgcgc ccggctcgaa aggcagaaca gccgcctcgc ccgcatattg gcgaatgagc
     4621 cgcccagccc cgctgtcgcc ttgcaggagc agcaattcgg gaaaccattt tttggaaaaa
     4681 acagccggca cgccgagtac gtccccatag cggcaggcag cgatggaact gttggttcgg
     4741 gattgtaaat caagtagttc cgacaatatt tgtgtggtaa caaaaggctg atcgctcaac
     4801 aaaaacaaca gattttccaa atcggggcga acagccaaag cctccatcac cgccaaccgg
     4861 atgctgcccg ccacgccctc ctgccactgc tcattgaccg ccacgcgaaa ctg
//
LOCUS       DAORLF010000373         4829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9628_length_4829_cov_1.663176, whole genome shotgun sequence.
ACCESSION   DAORLF010000373 DAORLF010000000
VERSION     DAORLF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9628_length_4829_cov_1.663176"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>243)
                     /locus_tag="PK198_21755"
     CDS             complement(<1..>243)
                     /locus_tag="PK198_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41440.1"
                     /translation="VDIAQPDTVCLPAGPLDLDAVVTGGGSQGQLVWSGPGITDTLAG
                     TWQPNAGMIGQLVPVTATFTEGPCSAADTVWIAVSKT"
     assembly_gap    244..341
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<342..>2932)
                     /locus_tag="PK198_21760"
     CDS             complement(<342..>2932)
                     /locus_tag="PK198_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41441.1"
                     /translation="GPDVTICNPGQSVNIGATVTGQYLSAVWTPSTGLSNPNALNTSA
                     TVSQTTTYTLTVSGLSASNLIVNGDFSAGLTGFTSDYVQGTGGSFGLLSLEGTFAVST
                     NANLTHNNFVNCTDHTGGGNMLVVNGAGTPNQNVWCQTVMVSPGTNYAFSLWGATVVA
                     SSPAILQFSINGNLLGSSFSLPTATCQWTQHFQLWNSGGASSATICIVNQNTQTSGND
                     FALDDIVFGPVCEETTQVTVTVIDVDATWTPPQGLCPLSPGFPLNSLLSPTATPGGAW
                     TINGSPATVFNPALLGPGTFSVSYTVGTAPCIETVTLPIVVNTLPSADWLTPGLMCVG
                     MPAVLLINLLLPGALPGGSWTINGSPAFVFNPSVWGPGSHTVIYETGTPPCTNSLAQI
                     IQVQAQPIADWTPPLPLCINSAAFPLNTLLPPGTTPGGTWLIGGNNALVFNPAVQGAG
                     PFSVTYQVGTFPCNASQTHFIEVLALPNAQWDGPDTLCRTAQIVMLNTWLLPGTATGG
                     SWTLDGQAATQLNPALLSPGLHTLVYTVGPANCQRSASGTVRIQDTLSAPEPFCSIVT
                     GNSVEIQWPGMPGQSYTVQVLSGPAPSFVNNTSAQFTGLAVGASVSVILQASAAGPCP
                     PVFSDTLTCTTFSCDALPVHIQPVDTLCLYPGAPAITLTATLPDTAAQGIPAWSGPGI
                     TNAVTGQFSPTVAGAGTHTIYFFYNLDNCVGTDSIEIVVVQRPTAMFSVAPIVCIADT
                     TVVTFTGMAGPSATYLWDFDDAQVLYGSGPGPIGLLWDSYGQHLISLTVEESGCVSLP
                     YFRPVNISAVVDTPQVFCNAALTEIELVWNSLPDGNSVIVYVLDGPLGTPTSDTSYLI
                     TGLMPGQ"
     assembly_gap    2933..2950
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3175..>4043)
                     /locus_tag="PK198_21765"
     CDS             complement(3175..>4043)
                     /locus_tag="PK198_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41442.1"
                     /translation="LSLGALREAVDFGRQAVDFADRSGEWGQKMIFRTTLADALHQSG
                     QSADAERLFGEAEAMQRERQPKYRFLYSLQGYRYCDLLLGRGKCAEVLVRANTTLKWV
                     MQANWLLDIAFDQFSIAQAHAAQSQEIPTSLAAAEQYFNHAVESIRRAEHSEFIAKGL
                     LARANWRLQTGRPTAALEDLDEVYEIAESGSMGLYHVDWHITMSRLQRMEGDDAAAAQ
                     HKEEALRRVRETGYLRRLGEAEELFMHVQTLQGLPNLAMSGHTTGHNDHKNLRLSASE
                     SETLTNLKDLSN"
     assembly_gap    4044..4053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4054..>4492)
                     /locus_tag="PK198_21770"
     CDS             complement(<4054..>4492)
                     /locus_tag="PK198_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41443.1"
                     /translation="WREANTRLYRYYKNLPDKHLPDTLEEMEPLFTAMAFGARAGLQQ
                     EVLDEVYWERIRWKGDSHSVKQLGAFGTDLAGLAALFEQPWSKPSSNLSKAGQAAVLN
                     WAGFRLRGLGRLREAVEPIRVSSIIAENLADWKNAATGASNLSE"
     assembly_gap    4493..4595
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4596..>4829)
                     /locus_tag="PK198_21775"
     CDS             complement(<4596..>4829)
                     /locus_tag="PK198_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41444.1"
                     /translation="KQPPVSPEVALLKLMGLFDRPAPVEALDILVKKPAIAGLTDGLA
                     SLPAERLTEALDNLKTLGLLETNRLKNSALPKN"
BASE COUNT          987 a         1290 c         1352 g          971 t
ORIGIN      
        1 gttttcgaaa cagctatcca aacggtatcg gcagcgctgc acggtccttc ggtgaaggta
       61 gccgtcacag gtacgagttg gccgatcata ccggcgttcg gctgccatgt tcctgctaaa
      121 gtgtctgtaa ttcccggccc gctccacacc aactgcccct gagagccacc gcccgtcacc
      181 acggcatcca gatcaagcgg cccggcaggc aggcacacgg tgtcgggttg ggctatgtcc
      241 actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttggccggg catcagcccg
      361 gtaatgaggt agctggtgtc tgaagtaggc gttcccagcg ggccgtctaa tacataaacg
      421 atcaccgaat tgccgtcggg caggctgttc caaacaagtt ctatttccgt cagggctgca
      481 ttgcaaaaga cctgcggcgt gtccaccact gcactgatat tgaccggcct gaaataaggc
      541 agggagacgc agccactctc ttccaccgtc agagaaatga ggtgctgccc atagctgtcc
      601 cacaacaatc cgatggggcc ggggcctgat ccatacagca cctgcgcatc gtcaaaatcc
      661 cataaatacg tcgccgaagg ccccgccatt cctgtaaaag taacaaccgt agtatcagca
      721 atacagacaa taggcgctac ggaaaacata gctgtcgggc gctgaacgac cacaatttct
      781 atagagtccg ttcccacaca attgtccaaa ttgtaaaaaa agtaaatggt atgcgttccg
      841 gctccggcca ctgtgggact gaactgccct gtcactgcat tggtaatacc cggaccgctc
      901 caggcgggaa tgccttgcgc agccgtatcg ggcagcgtgg ccgtcagagt gatggcaggc
      961 gcgccgggat acaggcacag tgtgtccacc ggctgaatgt gtacgggcag ggcatcgcag
     1021 gaaaaagtgg tgcaagtgag ggtatcgctg aataccggcg gacatggccc ggcggcggag
     1081 gcttgcaata ttacactcac gcttgcccca acggccaagc ccgtaaactg cgcggaggta
     1141 ttgttcacaa aggagggtgc cggtccgctc aacacctgca cggtataaga ctggccgggc
     1201 attccgggcc attgtatctc cacgctgttg ccggtcacga tgctgcaaaa tggctccggc
     1261 gcactcagcg tatcctgaat ccgtaccgtg ccggaagcgc tgcgttgaca attggccggt
     1321 cctactgtgt aaaccaaagt gtgcagtccg ggcgacaaca acgcggggtt cagttgtgtg
     1381 gcggcctgtc cgtccagtgt ccaggagccg ccggtagccg tgccgggcaa taaccaggta
     1441 ttgagcatca cgatctgtgc agtgcgacac agcgtatcag gcccgtccca ttgggcgttg
     1501 ggcaatgcca gcacttcgat gaaatgcgtt tgggaggcat tacatggaaa ggttcccacc
     1561 tgatacgtca ccgaaaacgg ccccgctccc tgcactgcgg ggttgaacac cagagcgttg
     1621 ttgcccccga tgagccaggt gccgcccggc gttgttcccg gagggagcag ggtgttcagc
     1681 gggaaggcgg cggagttgat gcacaacggt agcggcggcg tccagtcggc gatgggttgg
     1741 gcctgcactt gaatgatttg cgccagagaa ttggtgcagg gcggcgttcc ggtttcgtaa
     1801 ataaccgtgt gagaccccgg cccccagaca cttggattga atacaaatgc cggcgacccg
     1861 ttgatcgtcc aggagccgcc gggcaatgcg ccgggcagca ggagattgat cagcagaact
     1921 gccggcatac ctacgcacat aaggccgggc gtcagccagt ctgccgaggg caacgtgttt
     1981 accacaatgg gcaaggtcac ggtttctatg cagggcgccg tgcctacggt gtaagacacg
     2041 ctgaacgtgc cggggcctaa caatgcggga ttgaaaaccg tggcgggcga gccgttgatg
     2101 gtccatgcgc cgccgggagt agccgtaggc gacaaaagac tgttgagcgg gaagcccggc
     2161 gacagagggc acaaaccctg gggcggcgtc caggtggcgt ccacatctat gacggtgacg
     2221 gtgacctgcg tggtttcttc gcacaccggc ccgaaaacga tgtcgtccaa ggcgaagtcg
     2281 ttgccgctgg tctgggtatt ttggttcaca atacagatgg tggcagaaga agcgccgccg
     2341 gaattccaga gttggaaatg ctgcgtccat tggcaggtag ccgtcggcag tgaaaaagaa
     2401 ctgcccagca aattgccgtt gatggaaaac tgcaaaatgg ccggcgagga ggccaccacc
     2461 gttgcgcccc agaggctgaa tgcatagttg gtgccggggg ataccatcac cgtctggcac
     2521 catacgtttt ggttcggcgt tccggcgccg ttcaccacca gcatattgcc gccgccggtg
     2581 tggtcggtgc agttcacaaa gttgttgtgc gtcaggttgg cattggtgga aaccgcaaaa
     2641 gtgccttcca acgacagcag gccaaaggaa ccgccggtac cctgcacata atcgctggta
     2701 aaacccgtga ggcccgcgct gaaatcgccg ttgacaataa gattcgacgc gctcagcccc
     2761 gatacggtga gggtgtaggt ggtggtttgc gacacagtgg ccgaggtgtt cagggcattg
     2821 ggattgctca gtccggtgga gggagtccat accgcgctga ggtactggcc tgtcaccgta
     2881 gcgccgatgt tgacgctctg gccgggattg caaatggtga catccgggcc ggnnnnnnnn
     2941 nnnnnnnnnn gcgtgagcgt gaaggagcag gccatgttga gggcatggaa tccggggctg
     3001 ctgccggcca ttgccgaaat ggcccaatac aaaatacccc aagctgtaag aagtgtatag
     3061 cgtcttttca taatgatgtt ttaccgatct gccaaaggta attgtttttg ggaaatcggg
     3121 actgattgga acaggagggg gtgtgcgaaa tcagacgagg ttattggata ggactcaatt
     3181 tgacaaatct ttgagatttg tcaacgtttc gctttccgac gctgaaagac gcagattttt
     3241 atgatcgtta tgtcctgtgg tatgtccaga cattgcaagg tttggcaaac cttgcaatgt
     3301 ctggacatgc atgaacagct cctccgcctc ccccagccgc cgcaaatacc ccgtctctct
     3361 cacccgacgc agcgcctcct ccttatgctg cgctgcggct gcatcatctc cctccatccg
     3421 ctgcaggcga gacatggtga tgtgccaatc tacgtgatac aaacccatgc tgccgctctc
     3481 tgcgatctca tacacctcgt ctaaatcttc gagcgcagcg gtggggcgcc cggtttggag
     3541 ccgccagttg gcgcgggcga ggaggccttt ggcgataaat tcggaatgtt ctgctcttcg
     3601 aatactctct actgcgtggt tgaagtattg ttccgcggcg gcgagagaag tggggatttc
     3661 ttggctttgg gctgcgtgag cttgagcaat ggagaattgg tcaaaagcaa tgtctaaaag
     3721 ccagtttgcc tgcattaccc atttcaaagt tgtatttgcc cgcaccaaca cctctgcgca
     3781 ttttcctcgc cccaagagca gatcgcagta ccggtagcct tgcagcgaat acaaaaagcg
     3841 gtatttcggc tgcctttccc gctgcatggc ctccgcttcg ccaaagagcc gctctgcatc
     3901 ggcgctttgg ccggattggt gcagcgcatc ggcgagggtg gttctaaaga tcatcttttg
     3961 cccccactcc ccactccgat ctgcaaaatc caccgcctgc cgcccaaaat ccaccgcttc
     4021 gcgcagggcg ccgagactga ggtnnnnnnn nnngctcgct caggttggag gctccagtag
     4081 ccgcattttt ccagtcagcc agattttcag cgattatact actcacccgt atcggctcca
     4141 cagcctcccg cagcctgccc aagcctcgca gccgaaaccc tgcccaattc aacaccgccg
     4201 cttggcccgc cttgctcagg tttgaggaag gtttagacca aggctgttcg aacaaagccg
     4261 ccaatcccgc caaatccgtt ccgaaggcac ctagttgttt aacagaatga gaatcccctt
     4321 tccatctaat tctttcccaa tacacctcgt ccaacacttc ctgctgcaaa cccgcccgtg
     4381 ccccaaaagc catcgcggta aaaagcggct ccatttcttc caaggtgtcg ggcaggtgct
     4441 tgtcgggcag gtttttatag tagcggtaaa ggcgggtatt ggcctcgcgc cannnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggttt ttgggcagtg cggagttttt
     4621 gaggcggttg gtttccagca ggccgagggt tttgaggttg tcgagggctt cagtgaggcg
     4681 ttcggcgggc aggcttgcca atccatcggt cagtccggct atggcgggct ttttcaccaa
     4741 aatatccaac gcttcaacgg gtgcaggccg gtcgaagagg cccatcagtt tgagcaaagc
     4801 aacttcgggc gatacgggcg gctgctttt
//
LOCUS       DAORLF010000374         4809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9683_length_4809_cov_1.531132, whole genome shotgun sequence.
ACCESSION   DAORLF010000374 DAORLF010000000
VERSION     DAORLF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9683_length_4809_cov_1.531132"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>404
                     /locus_tag="PK198_21780"
     CDS             142..>404
                     /locus_tag="PK198_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41445.1"
                     /translation="MPTYLRITDISEGGHFLQNQMVSVVKGVTEEYYLSENDIVLART
                     GASVGKSYKYKVTDGRLVFAGFLIRVKPNPKKINSELLFQFFST"
     assembly_gap    405..506
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    770..800
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <801..1782
                     /locus_tag="PK198_21785"
     CDS             <801..1782
                     /locus_tag="PK198_21785"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41446.1"
                     /translation="SIEFKNRGKKGGKRDTLYFNAFTEDLFSWDNDLANDTERKLKIN
                     SSSTFFEGFRELALEEKIFAYLQRYAEFDFRIDYEEWTVTFSKGKETHIKISRGEENI
                     FIWCIFLAICELRLDDEKDEGPYKEVKYFYIDDPISSLDDNNAIAVASDLSKLLKRAN
                     GKVKTIISSHHSLFFNVMCNELKRLKPTQYFLHRNGMEGFGLQKTDDTPFFHHVALLS
                     ELKQAMETGEIKTYHFNSLRSILEKTSSFFGFNDFGKCIYGIEDQVLFERALNLFSHG
                     AYSIYNPVEMGDDNKELFKRVLRGFLDRHKFDLPELLNEKVKTAKLATTS"
     gene            1779..>2139
                     /locus_tag="PK198_21790"
     CDS             1779..>2139
                     /locus_tag="PK198_21790"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HRF41447.1"
                     /translation="MSEQNQQQLGKTLWGIADQLRGAMNADDFRDYMLSFLFLRYLSD
                     NYEAAARRELGSDYPTPAEGDRRAPLSLWYAANPEDVEAFEKQMRRKVHYVIEPAHLW
                     SSISELARTQHGEMLHTL"
     assembly_gap    2140..2184
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2185..>2716
                     /locus_tag="PK198_21795"
     CDS             <2185..>2716
                     /locus_tag="PK198_21795"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRF41448.1"
                     /translation="RTDFPDFNDPNKLGEGKAMVDRLTNLISIFEKPELDFSRNRAEH
                     DDILGDAYEYLIGQFAAGSGKKAGEFYTPQQISSILSGIVILDSQEPKTGPKPKLDKV
                     LDFACGSGSLLLNVRKQIGAKGIGKIYGQEKNITTYNLARMNMLLHGVKDTEFEIHHG
                     DTLLNDWDILNEMNPA"
     assembly_gap    2717..2785
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2786..>3176
                     /locus_tag="PK198_21800"
     CDS             <2786..>3176
                     /locus_tag="PK198_21800"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRF41449.1"
                     /translation="DFRFKGYGLAPKSAADFAFLLHGFHFLGKEGTMAIILPHGVLFR
                     GGVEERIRTKLLKDDHIDTVIGLPANLFYSTGIPVCILVLKKCKKYEDVLFINAAEYF
                     EKGKRQNALQPEHIAKIVAAYRERKEED"
     assembly_gap    3177..3226
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3599..>4809
                     /locus_tag="PK198_21805"
     CDS             3599..>4809
                     /locus_tag="PK198_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41450.1"
                     /translation="MKITYLYFFLLLSIVAQAQTAGLKGQLIDAGGEPVIYANVAVYN
                     AADSLLQKVETTDESGAFQIRGLRSGSYFVSASFLGMADLRKDGISLQEGQMLDLGSL
                     SFQAAAVELATATVTNSRAMVEVKPDRTVFNVDGTINSVGSDALALLRKAPSVTVDNN
                     DNINVLGRAGVLLYVDGKRLPLTGQDLSNYLQNLPADQIDRFDIITNPGAKYEAEGNA
                     GIIDIRLKRDKSMGANGSLSVSPSQGRYHRINTSATGNYRNKTMNAFANVGGSKGGGF
                     NDMRFRSLQNGLILNETNDNVNDWRGINLRAGADFFLSKNHTIGFLVSGNRGERESNS
                     INRIGISRQNTPEQIDSLLVAKTIGDNTNSQQTYNLNYRFDNAKGRSLNIDLDYGNYG
                     NTSIRLQPNQY"
BASE COUNT         1253 a         1175 c         1108 g          976 t
ORIGIN      
        1 ctgtgatttg ggaatgtgac gaatcattga gagaggtttg gagggtttga gggggagtgg
       61 gaggagaaga cgttggggga atgtttatta cgaaaaccgg actacggagt aaatgccccc
      121 gcagtaccat attctaaaga cttgcctacc tatttaagga taacagacat ttcagaaggt
      181 ggacatttcc tacaaaatca aatggtttcg gttgtgaaag gcgttaccga ggaatactat
      241 ttaagtgaaa acgatattgt acttgcaaga actggggcaa gtgtgggtaa atcatacaag
      301 tacaaagtta cagatggcag gcttgttttt gcagggtttt tgatccgtgt aaaaccaaat
      361 cctaagaaaa taaactcgga actgcttttt caattttttt ccacnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnccta taattttgcc gccaacactt gccgaacaac
      541 aaaaaatcgc cgcctgcctc accgccctcg acgagctcat cgccgcacgg ggcaggaggc
      601 tggagttatt gaaggagcag aagcgggggc tgatgcaggg cttgtttcct aacccgaatg
      661 aaatagaagc atgagtggca aacctaaaat acagtcatac aaggcactgc gtaatctggc
      721 agtaaggcta cgggacgacc taaatagtag cgattttgtg ctgctctatn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn ttctattgag ttcaaaaata gaggaaaaaa aggaggaaaa
      841 cgggatacac tttatttcaa tgcctttacc gaagaccttt ttagttggga caacgacttg
      901 gcgaacgata cggagaggaa acttaagatt aattcaagtt caacgttttt tgaaggtttc
      961 agggagttgg cgttggaaga aaaaatcttt gcctatttgc aacgttacgc cgaatttgac
     1021 ttccgaattg actacgaaga atggacggtg accttttcaa aaggtaagga aactcacatc
     1081 aaaatatcac gaggcgagga gaatattttc atctggtgca tttttcttgc catctgtgag
     1141 ctgaggctcg atgacgagaa ggatgaagga ccttacaaag aggtaaagta tttttatata
     1201 gacgacccca tttcgtcctt agacgacaac aacgccattg cggtggccag tgacctttcc
     1261 aaactactaa aaagggcaaa tgggaaggtg aaaactatta tttcatctca tcacagtttg
     1321 ttttttaacg tgatgtgtaa tgaactgaaa aggctaaaac ccactcaata ttttttgcat
     1381 agaaacggca tggagggctt tggcttacaa aaaactgacg acacgccttt ctttcatcat
     1441 gtcgccttgt tgagtgagtt gaaacaagca atggaaaccg gagaaataaa aacttaccat
     1501 ttcaacagtt tacgcagcat tctcgaaaaa acatcttcat tctttggttt taatgacttt
     1561 ggaaaatgta tctacggaat agaggatcaa gtactttttg agcgtgcgct caacctgttt
     1621 agtcatggtg cttattccat ctataatcct gtagaaatgg gggatgacaa caaagagctt
     1681 tttaaaagag tcctgcgagg attcttggac agacataagt ttgatttgcc cgagcttctt
     1741 aatgaaaaag taaaaacagc caagctggcc acaacatcat gagcgaacag aaccaacaac
     1801 aactcggaaa aacgctttgg ggcatagccg accaactgcg aggcgctatg aatgccgacg
     1861 attttcgcga ctatatgctg tcctttctct ttttgcgcta cctctccgac aactacgagg
     1921 ctgccgccag gcgggaactg ggatcggact accccacacc ggcggaaggc gaccgccgcg
     1981 ccccgctgtc gctgtggtat gccgccaacc cggaagacgt agaagcgttt gaaaaacaga
     2041 tgcggcgcaa ggtgcactac gtcatagagc ctgcgcattt gtggagcagc atttcagagc
     2101 tggcgcgcac ccagcacgga gagatgctgc acaccttgcn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnncccgca ccgacttccc ggacttcaac gaccccaaca
     2221 agctcggcga aggcaaggcg atggtggatc gcctgaccaa cttgatcagc atcttcgaga
     2281 agccggaact cgacttttcg cgcaaccgcg ccgagcacga cgatattctg ggcgacgcct
     2341 acgagtatct catagggcag ttcgcggcgg gcagcggcaa aaaagcaggc gagttttata
     2401 ctccgcagca gatttccagc atcctctcag gcattgtcat tttagacagc caggaaccca
     2461 agaccggccc caagccaaag ttggacaaag tgctcgattt tgcctgcggc tccgggtcgc
     2521 tcctgctcaa tgtgcgcaag caaatcggcg ccaaaggcat cggcaagata tacggtcagg
     2581 agaagaacat caccacctac aacctcgccc gcatgaacat gctgctgcac ggggtaaagg
     2641 ataccgagtt tgaaattcac cacggcgata ccctgctcaa cgactgggac attctgaacg
     2701 agatgaaccc cgccaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnngattt ccgcttcaag ggctacggcc ttgcccccaa
     2821 atcggcggca gacttcgcct tcctgctgca tggctttcac tttctcggca aggaaggcac
     2881 catggccatc atcctgccgc acggcgttct gttccggggt ggcgtggaag agcgcatccg
     2941 caccaagctg ctcaaagacg atcatataga tacggtcatc ggcttgccgg ccaacctgtt
     3001 ttactccacg ggcatcccgg tttgtattct ggtattgaag aagtgcaaaa agtacgaaga
     3061 cgtgctgttc atcaacgcag cggagtactt tgaaaaaggc aaacgccaaa acgctctgca
     3121 gccggaacac atcgccaaaa tagtggccgc ctaccgggag cgcaaggagg aagaccnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatc tgaacatttc
     3241 ccgctatgtc agcaccgcca aagaagagga agaagtggac ctgcaaaagg taaacgaaga
     3301 actattagag acagatcgaa aagcaaagga ggcgctggcg cggcacaatg cgttcttgcg
     3361 ggaactgggg ctgccgttac tgcccggttg aatgcaactc taatcccccc acatattata
     3421 cgccgtgtgc gtgttcccgc ccatgccggc tcctgaaact gaaccgacaa ggccggatac
     3481 cagaattgta ccgttcggtt caattacctc acccccttgc gcaacctttc cgcaactcat
     3541 ttgtctttgg caaagtcatt ttcaaattct ctttcaccca cctgactttg taaattgtat
     3601 gaaaatcacc tacctctatt ttttcctact cctgtccatt gtggcgcaag cccaaactgc
     3661 cggtctcaaa ggccagctca tagatgccgg cggcgaaccc gtcatctatg ccaacgtggc
     3721 cgtctataac gctgccgaca gcctgctcca aaaagtggag accaccgatg aatccggcgc
     3781 ctttcaaatc cggggactcc ggtcgggcag ctacttcgtg tcggccagct tcctcggcat
     3841 ggcagacctg cgcaaagacg gtatttccct gcaagaaggc cagatgctcg acctcggcag
     3901 tctcagcttt caggcggctg cggtagaatt agccacggca acggtcacca actcccgcgc
     3961 gatggtggaa gtaaaaccag accgcaccgt gttcaatgtg gacggcacca tcaacagcgt
     4021 aggctcggat gcactggcac tgctgcgcaa agcacccagc gtgacggtgg acaacaacga
     4081 caacatcaac gtgctgggcc gggcgggggt actcctctat gtggacggca agcgccttcc
     4141 gctcaccggg caagacctca gcaactacct ccaaaacctg cccgccgatc agatagaccg
     4201 attcgacatc atcaccaacc ccggcgccaa gtacgaggca gagggcaatg ccggcatcat
     4261 tgacattcgt ctgaaacgcg acaagagcat gggcgccaac ggttccctca gtgtctcgcc
     4321 gagccagggc cgataccacc gcatcaacac ttcggccacc ggcaattatc gcaacaaaac
     4381 catgaacgct ttcgccaatg tgggcggcag caaaggcggc ggcttcaacg atatgcgatt
     4441 ccggagcctt caaaatgggc tgattctcaa cgaaaccaat gacaatgtga acgactggcg
     4501 aggcatcaac ctccgggcag gcgccgattt ctttttatca aaaaaccata ccatcggctt
     4561 tcttgtaagt ggcaacagag gcgagaggga aagtaattcc atcaaccgaa tcggaatttc
     4621 cagacaaaac acgccggagc aaattgacag tctgctggta gccaaaacca tcggcgacaa
     4681 caccaacagc cagcagacct acaacctcaa ttaccgcttc gacaacgcca agggccgcag
     4741 cctgaacatt gacctcgact acggcaacta cggcaacacc agcattcgac tgcaacccaa
     4801 tcagtactt
//
LOCUS       DAORLF010000375         4807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9691_length_4807_cov_1.599958, whole genome shotgun sequence.
ACCESSION   DAORLF010000375 DAORLF010000000
VERSION     DAORLF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9691_length_4807_cov_1.599958"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1699)
                     /locus_tag="PK198_21810"
     CDS             complement(<1..>1699)
                     /locus_tag="PK198_21810"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41451.1"
                     /translation="GTAGATFNWTNNNPGIGIPGAGSGNINTPVITVLTTQVATFTVT
                     PQTAPDCIGTPVTFTVTVSPGTAPPIPGDTTMCRFSPLLTLPTSPGGVTGNWSGIGVV
                     NNVFNPMVSSGDLIQLLFVPNPGQCAVPATRNITFFPELLFFIGSFPGPYCEVSGPVP
                     LTGIIQPTGTPGTWSGPGVLPGNIFDPSLAGGGFHDISFTTAEACPVEVFFNIEVMDA
                     STPQFDIVGPYCPGDTPTVLPGTSNDGFTGTWSPAVIDTDMPGNTIYTFTPTDGQGCI
                     DEAVLNIVVQSPPTLTCAQQNPVTVTGGSDGSATVQISGGTPGYTIAWSGPVSGSQIQ
                     SAEGTATITNLMVGNYTVTVTDMLGCEQTCTFTIGDPGCNILLAATGSNPLCTGASNG
                     SIALTVSNSSGTLVFDWSNNTLDGQQNPSGLIAGTYSVTVTDGANCNASTSVTLSDPA
                     AIALTCAQQNPVTTVGGSNGSATVMITGGTAGYTVAWSGPSSGSQNQAAAGTATITGL
                     LAGNYTVTVTDADGGFSVQFMALPDGKTAKKFQPAFRFTVYADVTDIIDNAKDEPPF"
     assembly_gap    1700..1709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1882..>2090)
                     /locus_tag="PK198_21815"
     CDS             complement(1882..>2090)
                     /locus_tag="PK198_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41452.1"
                     /translation="EKLVDYYVFGKAKGLQVHHLFLIRPKSTSFIATLLPVFQNIYFN
                     NLRPLSVRHIFNFGLYLAPQNVYS"
     assembly_gap    2091..2152
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2153..>2496
                     /locus_tag="PK198_21820"
     CDS             <2153..>2496
                     /locus_tag="PK198_21820"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRF41453.1"
                     /translation="FFLIARVLQWKHLLWVLCFYIGSNYPSLQAQSFTENFDDITLLP
                     GNGWALVNASSPIGITGWFQGNNTVFSAFNGATDSYIGANFNNTTGANTISNWLMSPM
                     RTFNNGDVISFY"
     assembly_gap    2497..2656
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2783..2959)
                     /locus_tag="PK198_21825"
     CDS             complement(2783..2959)
                     /locus_tag="PK198_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41454.1"
                     /translation="MVVQLLLFTLYCRVEPAGQGVPVGAVMLPPDTTLLLQVLLTMPT
                     LAGAPLKLGQVAAV"
     gene            3783..>4235
                     /locus_tag="PK198_21830"
     CDS             3783..>4235
                     /locus_tag="PK198_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41455.1"
                     /translation="MAGTTYYVYVHPADDFGNGTSDFCFTVTTTLPPCPTFSGAPANV
                     GIVNSTCGSGCTLTGGSITAPTGTPCPAGSTLQYNVNNSGWTTSLPVYDQDGPAQTIR
                     TRCACDNDANNVSAESNPVSTVPGTLANPVVPANGSAIVACPALATQPT"
     assembly_gap    4236..4313
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4314..>4807
                     /locus_tag="PK198_21835"
     CDS             <4314..>4807
                     /locus_tag="PK198_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41456.1"
                     /translation="VTCEGTRTYTWTYSCGSTSSTWSFVYTIERNPFTVPANGAATVA
                     CLAAITVPVPPAVNSNCGEPIAPAFVSIVDSPTPLTCEGTRTYNYSYTDCEGNTLLWS
                     FVYTIERNPFTVPANGAATVNSLTLATQPTPPTVTSNCGEVLTPTGPVVTNVPDPIVC
                     TGTR"
BASE COUNT          986 a         1236 c         1256 g         1019 t
ORIGIN      
        1 atgaaaggcg gttcgtcttt ggcgttatcg atgatgtcgg tcacatcggc atacaccgtg
       61 aagcggaagg ccggctggaa ttttttggcg gttttgccgt cggggagcgc catgaattgc
      121 accgaaaacc cgccgtcggc atcggtgacg gtgacggtgt aattgcctgc caataagccg
      181 gttatggtag cagtgccggc agccgcctga ttttgagagc cgcttgaagg gccgctccat
      241 gcaacggtgt agcccgccgt gccacccgtg atcattacgg tagccgaacc attggagccg
      301 cctaccgtgg tgacagggtt ttgctgcgca caagtgagag ctatggccgc ggggtcgctg
      361 agtgtgactg aagtacttgc attacagttg gcgccgtcgg tgacggtgac ggaataggtt
      421 cctgcaatca gcccggaggg attttgctgc ccgtccaatg tattgttgct ccagtcaaaa
      481 accaaagtgc cgctgctgtt gctcaccgtc agcgcgatac tgccgttgga agcgcccgta
      541 cacaatggat tactgcctgt ggcggccaaa aggatattac aacccgggtc tccgatggtg
      601 aacgtacagg tttgttcgca gcccagcata tcggtgaccg tgacggtgta attgcctacc
      661 atgaggttgg tgatagtggc cgtaccttct gcgctttgta tttgagaacc gcttaccggg
      721 ccgctccatg caatggtgta gccgggcgtg ccgccgctga tttgcaccgt agccgagccg
      781 tcggagccgc ctgttacggt tacgggattt tgctgcgcac aagtcagggt tggaggagac
      841 tgcactacta tattcaatac agcctcgtct atacagccct ggccgtcggt gggcgtgaat
      901 gtatagatgg tattgccggg catgtcggtg tctatgaccg ccggcgacca tgtgccggtg
      961 aagccgtcat tggacgtgcc gggcaacacg gtgggcgtat ctccgggaca ataagggccg
     1021 acaatgtcaa attgtggggt actggcatcc atcacctcaa tattaaaaaa tacctctacc
     1081 gggcaggctt ccgcagtagt gaaagatatg tcgtgaaaac ctccaccggc caagctcgga
     1141 tcgaaaatat tgccgggcaa tacgccgggg ccgctccagg tgcctggtgt tccggtgggt
     1201 tgtataatgc cggtgagtgg aacggggccg cttacttcgc aatacgggcc ggggaaggag
     1261 ccgatgaaga acagcaactc aggaaaaaac gtaatgttgc gggtggccgg cacagcacat
     1321 tggccgggat tgggtacaaa caacaactgg atgaggtcgc cggaggagac catggggttg
     1381 aacacgttgt tcactacccc gatgcccgac cagttgccgg taaccccgcc cggcgaggtg
     1441 ggcaaagtca gcagcggcga aaagcggcac atcgtggtat cgcccggtat ggggggcgcc
     1501 gtacctggag ataccgtgac agtgaaggtg accggcgtgc cgatgcaatc cggggctgtt
     1561 tgcggcgtaa cggtaaaggt ggccacctgt gtggtcaata cggtaatgac cggggtgttg
     1621 atgttgcctg aacctgcgcc gggtattccg ataccggggt tgttgttcgt ccagttgaaa
     1681 gttgcccctg ccgtgccgcn nnnnnnnnng aacacgacca tcagattttg attggagcac
     1741 aacatgatac tggcaggcgc ggtcacaacc ggcggactag ggcattgtgc agatacggag
     1801 gctatacttc cgtatgaaag gcaggtcatc aacaaaaagc gcacgagacg caggctggca
     1861 atcggcattg ggaaacaggt tttaagagta aacattttgc ggggccaaat ataggccgaa
     1921 attgaatatg tgccttacag atagtggcct gaggttgtta aaatatatat tttggaaaac
     1981 aggaagcaat gtagcgatga aacttgtact tttcgggcgg ataagaaata aatgatgcac
     2041 ctgaagacct ttggctttgc caaaaacata ataatccacc aatttttctc nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtttcttc
     2161 ctcatcgccc gcgtgctcca atggaagcac ttgctttggg tgctatgttt ttacatcggg
     2221 agcaactacc catccttgca ggcccagtcg tttacagaga atttcgacga catcaccctg
     2281 ctgcccggca atggatgggc attggtcaat gccagttcgc caataggtat taccggctgg
     2341 tttcaaggaa ataacactgt tttttctgcg ttcaatggcg ctacagattc ctacattggc
     2401 gccaatttca acaataccac cggtgcgaac accatcagca actggctcat gtcgccgatg
     2461 agaaccttta acaacggcga tgtcatcagt ttttatnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnngttt tctgcgaccc tctccggctt gccccccggc ggtgcgagcg
     2701 gtcgctttgc gctcaagtac gatgttacca atggcggccc taccggggct aattcggatt
     2761 acatcggtat agacaacgtg gtttatacgg ctgccacttg ccccagcttc agtggcgcac
     2821 cggccaatgt cggcatcgtc aacagcacct gcaacagcaa tgtggtatcc ggcggcagca
     2881 tcacggcgcc taccggcacg ccttgtcctg cgggttctac cctgcaatac aatgtgaaca
     2941 acagcagctg gacgaccacc ttgcctgttt acgcccaaac aggcccgacg caaagcatcg
     3001 taacgcgctg cgcttgcgac agtgacggca ctatggtaag tgcagaatcc accgctgtaa
     3061 cgacggcgcc gggcgtttgt agcccgccaa gcccctgcga tgtcatcacc ccactgcctt
     3121 gcggaacagc tgtctcccca agtctttctg gcactggatt ttggaatact tcattttgtg
     3181 gtaattccac agttggctca gaggcggtct atagttttac cccaaccatg tcaggtgttc
     3241 acacacttat catcaatggc gttggaggcg accccgttga ctttggctac aagctggcgt
     3301 ccgatggttg ttcctcaagt ggttggaact gcattggtac ttctaccggc accagttatt
     3361 acacttttgg cccactgacg gctggggttc agtattacat cttactggat gcgcacacga
     3421 ccgctttttc tgcgcaaaat tttgaaatca actgtaacat agttcccgca aacgaccttt
     3481 gcgtcaatgc catcaccata cccgctgtga ccggcaatat atgcggaaca acccttggcg
     3541 ccaaccccga catccccaat agcggtgcaa cttgtggaac cagcgctgat gggttcggtg
     3601 ccggcgtttg gtacacattt caaggtaatg gccaggattg gagctttttc ttccctcagc
     3661 aaggttcaag ttgggaccca gaggtaaacg tttactcggg atcctgcggt tcgctgactt
     3721 gtgtcactgg cgacgatgac agtggtggcg gactcaatgc acagtttacg gtaagtaata
     3781 ccatggcagg caccacatac tatgtctatg tgcatcctgc agatgatttt ggcaacggaa
     3841 cgagtgattt ttgcttcacc gtcactacta ctcttcctcc ttgccccacc ttcagcgggg
     3901 cgccggccaa tgtgggcatc gtcaacagca cctgcggcag cggttgtacg ctcacgggcg
     3961 gcagcatcac agcgccgaca ggaacaccct gcccggcagg ctctaccctg cagtataatg
     4021 tgaacaacag tggctggacg acgagcttgc ccgtttacga tcaggacggc ccggcacaaa
     4081 cgatccgcac gcgctgcgcc tgcgacaacg acgccaataa cgtcagcgct gaatccaatc
     4141 cggtgagcac cgtgcccggc acattggcca atccggtagt acctgccaat ggttcggcta
     4201 tcgtagcctg cccggccttg gccacacagc ccacgnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgttacc
     4321 tgcgagggta cgcggacata cacctggaca tactcctgcg gaagcacatc ttccacctgg
     4381 agctttgtct ataccatcga gcgcaatccg tttactgttc cggccaatgg agctgcgacg
     4441 gtcgcttgtc tggcagccat tacagtgccc gtgccgccgg cggtcaacag caactgcggc
     4501 gagcctattg caccggcttt tgtatcaatt gtggatagcc ctactccgtt gacctgtgaa
     4561 ggaacccgca cttacaacta ttcttacaca gactgcgagg gcaatacgct gctctggagc
     4621 tttgtctata ccatcgagcg caatccgttt acggttccgg ccaatggcgc cgctacggtc
     4681 aactctctga cattggccac ccagcccacg ccgcctactg tgacgagcaa ctgcggcgag
     4741 gtactgacgc cgacaggtcc ggtggtgacc aacgttccgg atccgattgt ctgcaccggc
     4801 acccgga
//
LOCUS       DAORLF010000376         4801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9707_length_4801_cov_1.795617, whole genome shotgun sequence.
ACCESSION   DAORLF010000376 DAORLF010000000
VERSION     DAORLF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9707_length_4801_cov_1.795617"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="PK198_21840"
     CDS             complement(<1..1690)
                     /locus_tag="PK198_21840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF41457.1"
                     /translation="MTGYLRLLFVLISIIGISRPLATAQETFPINGIYDQREGLYAFT
                     NATIYTAYNERLDKATLLIRDGKVEAVGLKVTVPTNAVVIDLKGKTIYPSFIDMYSDY
                     GMPAMRQDGAAGGRGFGGSQQHLSSKKGAYNWNEALKPEFRAHEVFTADEKTAKTLRD
                     IGFGAVLSHQHDGISRGSSTLVTLADDREHALIVKDKAAHHFSFRKGSSRQGYPGSLM
                     GAIALLRQTYYDAQWNKTNKDEYNISLAAWNDLAALPQVFEVSDWQEVLRAVNLGKEF
                     NVKYIIKGGGDEYKRLDEIKKAGAPLILTLNFPEAFDVEDPIDARQVGMSQLKHWEMA
                     PSNPAQVAAAGIPFVLSSHGLRNKAEFMAQLRKAMEYGLSEADALKALTHTPATLMGS
                     ADRIGSLSPGKWANFFITNGNVFSKDAKIYHHWVNGKPYLLKDLDSPALTGKYTLTMT
                     TTANGVQYGQYNLIVTGTDENPEMKLQLNDTSSVKIKHSYSKGMITLSFAMKEGDPRV
                     RLSGTVSGKNWSGTGEDDKGHTTAWSVSPSAGTEAAAETKKATNPLPSPKSALPT"
     assembly_gap    2068..2178
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2179..2544)
                     /locus_tag="PK198_21845"
     CDS             complement(<2179..2544)
                     /locus_tag="PK198_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF41458.1"
                     /translation="MEQLKKTHRQMGWQLLLNEHRMLNIKGENVAVIGVENYSTHLRF
                     PKYGKLEQAHKGTQNAALRLLLSHDPSHWKGQVIQDFQDIDITFSGHTHGMQFGVEIP
                     GVFKWSPIQYVYKEWAGLYS"
     assembly_gap    2592..2601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2602..3335)
                     /locus_tag="PK198_21850"
     CDS             complement(<2602..3335)
                     /locus_tag="PK198_21850"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF41459.1"
                     /translation="MRLIIFTGILLAIDLYAFQAFRTATQNWSVGWRLALNIIYWLVP
                     VLMLAYLLAAQADYTEHWPKTVTTLARSFFFIAYFSKFLIASVILADDLRRGSMWVYE
                     KIAGREPFDYSRSRFLSNMGLILGGIPFVSLTYGILRNPYRFKLYSQKVKIEGLPETL
                     QGLRIVQISDIHSGSFLYRDPVTAAVDLINAQQPDLVFFTGDLVNNVATEMERFTDVF
                     DKIQAKYGVFSILGNHDYGDYVSWESP"
     gene            complement(3420..>4801)
                     /locus_tag="PK198_21855"
     CDS             complement(3420..>4801)
                     /locus_tag="PK198_21855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41460.1"
                     /translation="KNKAPLWHLGGAAAFFMALMSKENAITFLAIIPLTFYFFTKADW
                     KQTLLHSLPAWGAALLFLLIRGSVLGWKLGDPPLELMNNPFLKIENGQWVAFSASEKM
                     ATIMVTLWEYLRLLFVPHPLTHDYYPRHIDIGSWGQWKALAGLGLHLALGVYALLRLR
                     GKDPVSYGILWYLITLSIASNIVFPVGTNMSERFIYMPSVGFGIALSVLLYRQFKTPD
                     GNRLSTGLAIIGVAALLFSVKTIMRNGVWKDNYTLFTTDVKNAPNSAKLRNAAGGELF
                     TQAIKPENEKRREAMLREAVPHLQEAIRLHPTYKNAYLLLGNCLNYLKDYDASIAAFR
                     GALAIDPNYKDAQENLFVTYRDAGKYFGEQRGDLAKSLQMLGEAVQMKSDDFETLRLL
                     GVANGVGGNMPVAEQYFNKALEINAGHADTWFDLGITYLSMGNTARHNECIAKAQQLE
                     PGILERRKK"
BASE COUNT         1003 a         1191 c         1333 g         1153 t
ORIGIN      
        1 tagttggcag cgccgatttc gggcttggca gcgggtttgt cgcttttttc gtctccgcgg
       61 cagcttcagt tccggcagat ggcgagaccg accaagcggt cgtgtggcct ttatcgtctt
      121 cgccggtgcc cgaccagttt ttgcccgaaa ccgtgccgct gaggcgcacg cggggatcgc
      181 cttctttcat ggcgaaagac agcgtgatca tgcctttgct gtagctatgt ttgattttga
      241 cagagctggt atcgttgagt tgaagtttca tttccgggtt ttcgtcggtg ccggtcacga
      301 tcaggttgta ctgcccgtat tggaccccat tggcagtggt cgtcatggtc agggtgtatt
      361 tgcctgtcaa tgccgggctg tccaaatcct tgagtaggta tggtttgccg ttcacccagt
      421 ggtggtaaat tttggcgtct ttggaaaaca cattgccatt ggtaatgaag aagttggccc
      481 atttgcccgg cgagaggctg ccgatgcggt cggctgagcc catgagcgtg gccggcgtat
      541 gggtcagggc tttgagggca tccgcttccg agaggccgta ttccatggct ttgcgcaatt
      601 gggccatgaa ttctgcttta ttgcgcaaac cgtgcgaact gagcacaaac ggaatacccg
      661 ctgcggctac ctgcgccgga ttggagggcg ccatttccca gtgcttgagc tgagacatgc
      721 ccacctggcg cgcatctatg gggtcttcca cgtcgaaggc ttcagggaaa ttgagcgtca
      781 ggatgagggg tgcgccggct tttttgatct cgtccaagcg tttgtactcg tcgccgccgc
      841 ctttgatgat gtatttcaca ttgaactcct tgcccagatt gacggcgcga agtacttcct
      901 gccagtcgga cacttcaaat acctgcggca gtgcggccag atcgttccag gcggccagcg
      961 agatgttgta ctcgtctttg ttggttttgt tccactgagc gtcgtaataa gtctggcgca
     1021 gcagcgcaat ggcgcccatg agcgagccgg gatacccctg gcgggaactg cctttgcgga
     1081 aggagaaatg gtgcgccgct ttgtccttca cgatgagggc gtgttcgcgg tcgtcggcca
     1141 gcgtgacgag agtggaactg ccacgcgaga tgccgtcgtg ctgatggctg agtactgctc
     1201 cgaaacctat atcgcgcagg gttttggctg ttttttcgtc ggcggtaaag acctcgtgcg
     1261 ccctgaattc cggcttgagc gcctcgttcc agttgtaggc gcctttcttc gagctgaggt
     1321 gctgctggga gcctccgaag ccccttccgc ctgccgcgcc gtcttgtcgc atagcgggca
     1381 tgccgtagtc ggaatacata tcaatgaagg aaggatagat ggttttgcct ttgagatcaa
     1441 tgacaacggc gttggtcggc acggttactt tcaggcccac cgcctccact tttccgtcgc
     1501 ggatgagcag ggtggctttg tccaagcgct cattataggc agtgtaaatg gtggcattgg
     1561 tgaaggcgta gaggccctcg cgttggtcgt agatgccgtt gatcgggaag gtttcctgtg
     1621 cagtagcgag tggacgggag atgccgatga tggaaatcag cacgaagaga agtcgcagat
     1681 agccggtcat agtcggatgt cgtgattttg gtagtgaact tatagatagc attcttcaaa
     1741 aggctgccgg cccttagcca caagccctta gcttgtcttc tgttgagaaa tcagatttcc
     1801 catttgggaa aatatcaaaa tgtactcttg aaaaagtttt catttttcat ttccgacagc
     1861 caaccccgcc tcgctgaaag gctttcaggc cgacgtgaac agatacacaa gctttcaaag
     1921 gacaattgta aggagttttt acggaatgcg caaaggattt tgcacggcaa tcacatttag
     1981 cccgcaaagt gcgcagcgta ctccctgata gggggatcgc ctgttgcgac gattcagaaa
     2041 acggggctgt ttatgtctat tctcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnac tgtacagccc ggcccattct ttgtaaacat attgaatcgg
     2221 gctccatttg aacacgcctg gaatttccac cccaaactgc ataccgtgcg tatggcccga
     2281 aaaagtgatg tctatgtctt ggaagtcctg tattacctgg cctttccagt gcgagggatc
     2341 gtgtgaaagc aatagccgga gggccgcgtt ttgagtgcct ttgtgcgcct gttccagctt
     2401 gccgtatttg ggaaaacgca ggtgtgtgga gtagttttcc acgccgatga cggccacgtt
     2461 ttcacctttg atgttcaaca tcctgtgctc attgagcagc agttgccagc ccatttggcg
     2521 gtgcgttttt ttcagttgct ccatgttttg agccttcttt tcaggcgatt gctcaactga
     2581 tgcaggtact tnnnnnnnnn nggcgattcc cagcttacgt agtcgccgta atcgtggttg
     2641 cccaaaatgg aaaacacgcc gtatttggcc tgtattttat caaaaacatc ggtgaagcgc
     2701 tccatttccg tagccacatt gtttaccaaa tcgcctgtaa aaaagacgag gtcgggctgc
     2761 tgggcattga tgagatcaac ggcggccgtc acggggtcgc ggtagaggaa actgccggaa
     2821 tggatatcgg aaatttgcac tatgcgcagc ccctgcaagg tttcggggag gccctcgatt
     2881 ttgacttttt ggctgtacag cttgaaccgg tatgggttgc gcaggatgcc gtaagtgagc
     2941 gaaacaaacg ggatgccgcc caatatgagg cccatgttgg acaaaaagcg ggaacggctg
     3001 taatcaaagg gttcgcgccc ggctattttt tcatacaccc acatagaacc ccgccggagg
     3061 tcgtcggcca aaatgacgga agcaatgagg aatttggaaa aataggcaat gaagaagaag
     3121 ctgcgcgcca gggtggtgac tgttttgggc cagtgctcgg tgtaatcggc ctgagccgcc
     3181 aacaaatatg ccagcatcag caccggtacg agccagtaaa taatattgag tgccagcctc
     3241 cagccgacag accaattttg tgtggcggta cgaaatgcct gaaaggcgta gaggtcaatg
     3301 gccaggagga tgcctgtaaa aatgatcaat cgcatgggta tcgaaattgc ttgttccggc
     3361 tctacacgcc gacaagagtg cccatagtaa tgccgaaacc ctgctatttg ttttgggcct
     3421 tattttttcc tccgctccag aatgcccggc tccaattgct gcgctttcgc aatacactcg
     3481 ttgtggcggg ccgtattgcc catgctgaga taggtaatgc cgagatcgaa ccaggtatct
     3541 gcatgtccgg cattgatttc cagcgcctta ttgaagtatt gctctgccac cggcatattg
     3601 ccgcccacac cgttggccac ccccagcagg cggagggttt caaaatcgtc ggacttcatt
     3661 tgaacggctt cgcccagcat ttgcaaggat ttggccagat cgccgcgctg ctcgccgaag
     3721 tacttgcccg cgtcccggta ggtaacgaag agattttcct gcgcatcttt ataattagga
     3781 tcaatggcca acgcgccccg aaacgcagcg atggaagcat cgtaatcttt gagataattg
     3841 aggcaattgc ccagcagcag gtacgcattt ttgtaagtgg gatgtaagcg gatggcttct
     3901 tgcaggtgcg gcacagcctc ccgcagcatg gcctcgcggc gcttttcgtt ttcgggtttg
     3961 atggcctgtg tgaacagttc gccgcctgca gcattgcgca gtttggcact gttgggcgca
     4021 ttttttacat cggtggtgaa gagcgtgtaa ttgtctttcc acacgccgtt tctcatgatg
     4081 gtttttacag aaaacagcag ggctgccaca cctatgatcg ccaggccggt tgacagccgg
     4141 ttgccgtcgg gcgtcttaaa ctgccggtac aacaacaccg acaacgcgat gccgaatccc
     4201 accgagggca tgtaaataaa gcgctccgac atgttggtgc ccaccggaaa aacgatattg
     4261 gatgcaatgg acagcgtgat gaggtaccac aaaatgccat aactcacggg gtctttgccg
     4321 cgcagccgca agagcgcata cacgcccagc gcgaggtgca ggcccagtcc agccagcgct
     4381 ttccattgcc cccaggaacc gatgtctatg tggcgggggt agtagtcgtg cgtcagcggg
     4441 tgcggcacga acagcaagcg caggtactcc cacagcgtga ccatgatggt ggccattttt
     4501 tcacttgcgc tgaacgccac ccattggccg ttttcgattt tcaggaatgg attgttcatc
     4561 aattccagcg gcggatcgcc gagtttccaa cccaaaaccg agccgcgaat gagcaaaaac
     4621 agcagggccg caccccaggc cggcaggctg tgcaggaggg tttgcttcca gtcggccttg
     4681 gtgaaaaagt aaaaagtgag cggaataatg gccaaaaacg tgatggcatt ttccttcgac
     4741 atcagggcca taaaaaatgc cgccgcaccg cctaaatgcc acagcggcgc tttgttttta
     4801 a
//
LOCUS       DAORLF010000377         4798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9715_length_4798_cov_1.973856, whole genome shotgun sequence.
ACCESSION   DAORLF010000377 DAORLF010000000
VERSION     DAORLF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9715_length_4798_cov_1.973856"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>369)
                     /locus_tag="PK198_21860"
     CDS             complement(<1..>369)
                     /locus_tag="PK198_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41461.1"
                     /translation="ELVVINDGDPPQAIADNGGPYCPGSPISLFGNYSWSGAPGPVMY
                     QWSGPGGFSSNQQNPTNATQDGTYNLVVTVNGCPAPPASTTVVFNPAAANATASPATV
                     CPGQPATLTATGAVSYVWDTG"
     assembly_gap    370..441
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<442..909)
                     /locus_tag="PK198_21865"
     CDS             complement(<442..909)
                     /locus_tag="PK198_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41462.1"
                     /translation="MQTFGPGFAYDSAAGLGCGGTPNDGNPGNNWGDGNNGCANIGGA
                     ANPSVTFCWSITVGNCPPNTTGNSLSVIVNVLSDGDSGSWNQVGCNSGNQYNFLASAV
                     CCDDGNPFVTDTPTRCPGVSDGTITMEGSTTGKPYNFFLFNSMIKTIILFNILC"
     gene            complement(878..1651)
                     /locus_tag="PK198_21870"
     CDS             complement(878..1651)
                     /locus_tag="PK198_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41463.1"
                     /translation="MKSTLTFLFLLCVTLALAQPPNDECGGAIPLPLGTPSACPNSTP
                     VTNTFNVTNVNATPTTPYPTFSNCSIGNQTSAPADEVWFSFVPNSNIMSITVGAGLSS
                     PNIVIFTGNNCSFLTAVECARGPAPLTLTLPVVPNQQYYMLISGGAIGDQANFTLTMT
                     SSRDCNPCLQSANFVAFPPPLNGTYNSGQTVSFCYTITQWNVTGTIEWLHALEVEFGP
                     GWDLNSFSPTPPPSCDGQGSWNWYDGWVSCANFRAGVCL"
     gene            1798..2373
                     /locus_tag="PK198_21875"
     CDS             1798..2373
                     /locus_tag="PK198_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41464.1"
                     /translation="MRRLILIAFLISAPAVAQLQIYLLNASFEGEPQDATVPSGWIPC
                     QAGTTPDILPGVWGVYQEPVHGDTYMGLITRENGTWESVGQRLKTALSPGQCFAMSLS
                     LAHSNTYAGYKLPLKLRIWGGAERCEQDQLLWESPLIEHREWKTYRFDFVPRASIQYL
                     IIEAFHQEGNFSYRGNILIDRLSAIKKCIRA"
     gene            2465..>4096
                     /locus_tag="PK198_21880"
     CDS             2465..>4096
                     /locus_tag="PK198_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41465.1"
                     /translation="MKISSFLLFAATVLLAPTRLLAQDCNTFIEKELTVGGLHLLRTI
                     PLTMVIRGTYTYSIQIINDEKGLRAKVISRAGVEFNQDDEIIFMDVNSLRKGYRFMEM
                     GEMKEEGGIPEYHNFLQINLDAIQWFSEVDINTIYIKNNLSKEMRKFTVNPNRMADFR
                     MLASCFDQRLNKTNVVNVTNAERDYTKPGANDTGLLTAKPETPTPGKPAATPGKPAVN
                     TPAAASGSDAELDALRRDLAATKEQIRKEIALEKEKAQRYRQQLQDELSLDRENAARQ
                     KKQYADEVLDARQKAQAQMEESKRALADSIRLARANAGSQIEKINLEVQESRQRAAEA
                     VQKAQLESAKEVAAARDKAAAEVKTLKDKMEQAKLQYTDEIASARENSNRELERIRQE
                     SSDQVRQAREAAQQQSSATMEQVAESRETASALILRTKEEAAAEIARTRENAVLEKER
                     IAVEVAESRRKGAEEIALARQNAAAETAAIAERTSTEKEAAAREVAETRQRAAEQVAQ
                     IKKQVAEEEQKGQQQTEAARQEAAQSVAEARRQAAL"
     assembly_gap    4097..4130
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4131..>4798
                     /locus_tag="PK198_21885"
     CDS             <4131..>4798
                     /locus_tag="PK198_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41466.1"
                     /translation="KIAEARQKSEAERKTIADEVAAARQRALDEIKKIQEESAHAMAE
                     TKAAETKMREQSAQEVAAARQRAADEVKAAQAKAAAQVAEIESKLENSRQQYTQEVGN
                     AQKTAQERLAQIQKEAAAAIADAKSKEQEAKNTSVEEVVVTRENAAREIAKARETAAL
                     EIQKAREQAVLAQQESANAAAAARKSSADAVAQAQQEESRQIKELREKSAAEKQALAA
                     EVAQ"
BASE COUNT         1224 a         1216 c         1345 g          907 t
ORIGIN      
        1 ccggtatccc acacatagct gaccgccccg gtagccgtca gcgtagcggg ttggccgggg
       61 caaacggttg caggcgatgc cgtagcattg gcggcagccg gattaaacac tacggtagtg
      121 ctcgccggcg gggctgggca gccgttgaca gtaactacta aattgtaagt gccgtcctgt
      181 gtagcgttgg tggggttttg ttggttggaa ctaaagcctc cggggccgct ccattgatac
      241 atcaccgggc ctggagcgcc gctccatgag tagtttccaa acagggaaat aggggagccg
      301 gggcagtaag gtccgccgtt gtcggcaatg gcttgtggag gatcgccgtc gttgatgacg
      361 accaattcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn ngcagagtat attaaagaga atgattgttt tgatcatact
      481 gttgaaaagg aagaagttgt aaggtttgcc cgtcgtgctg ccctccatgg tgatggtgcc
      541 gtcggatacg ccggggcagc gggtaggagt gtcggttacg aaggggttgc cgtcgtcgca
      601 gcaaacagcc gaggcgagga agttgtattg gttgccggag ttgcagccca cctgattcca
      661 ggagccggag tctccgtcag ataatacgtt gacgataaca ctgaggctgt ttccggtggt
      721 attggggggg caatttccca ccgtaataga ccagcaaaac gtaacggaag ggttggcggc
      781 gccaccgata ttggcacagc cgttgttgcc atcgccccag ttattgccgg ggttgccgtc
      841 gttgggggtg ccgccgcagc ccaatccggc tgccgaatca taggcaaacc ccggcccgaa
      901 agtttgcaca actgacccag ccatcgtacc agttccagga accttggccg tcgcaggaag
      961 gcggaggagt ggggctgaaa gaatttagat cccagcctgg cccaaactcc acctccaacg
     1021 catgcaacca ctcaatggtg ccggtcacgt tccactgggt gatggtgtag caaaaactga
     1081 cggtttgtcc cgagttgtag gtgccgttga gcgggggagg aaaggcaaca aaattggccg
     1141 attgcagaca cgggttgcaa tcccgcgaac tggtcatggt gagggtgaag ttggcctgat
     1201 cgccgatggc cccacccgaa atgagcatat aatattgctg gttgggcact accggcagcg
     1261 tcagggtcag cggcgcaggc ccgcgcgcac attccacggc agtgaggaaa gagcagttgt
     1321 tgccggtgaa aatgacgata ttgggagacg acaagcccgc tcccaccgtg atggacataa
     1381 tattactgtt gggcacaaac gagaaccaca cctcatcggc cggggcgctg gtttggttgc
     1441 ctatggagca gttgctgaaa gtagggtagg gcgtggtagg ggtggcattt acgttggtca
     1501 cattgaaggt attcgtgacc ggtgtggagt tggggcaagc cgaaggtgtg ccaagtggga
     1561 gtgggatagc gccgccgcat tcgtcgttgg gaggctgagc caaagctaat gttacgcaaa
     1621 ggagaaagag aaaagtaagt gttgatttca tgttgtcgct gtttgtagct tacttatagt
     1681 gtagtgttac aaaggtacga atcgcagcga gaaagctgcc tgagccgcgc gctgcccgac
     1741 aaaaaaaatg ctattttata aaaagggcta actttgcttt gtgaataaat acataccatg
     1801 agacgcttga ttttaatagc ttttttgatc tcggcccctg ctgtggcgca actccagata
     1861 tatctgctca atgcttcttt tgaaggagaa ccgcaagatg ccactgtacc ttcgggctgg
     1921 attccctgcc aggccggcac cacccccgat atattgcccg gcgtgtgggg agtctatcag
     1981 gagcctgtgc acggcgacac ttacatgggc ctcattaccc gcgaaaacgg cacctgggag
     2041 agtgtgggcc aacgcctgaa aacggcgctc tcgccagggc agtgtttcgc tatgtccttg
     2101 tcgttggcgc attccaatac ctacgccggc tacaaactgc cgctcaaact gcgcatctgg
     2161 ggcggcgccg aacgctgcga gcaagatcaa ctgctgtggg aatctccgct catagagcac
     2221 cgcgaatgga agacgtatcg cttcgatttt gtgccccgcg cctccattca gtacctcatt
     2281 attgaggcgt ttcatcagga gggcaatttt tcttaccggg gcaatatcct catagaccgg
     2341 ctttctgcca ttaaaaagtg catccgcgcc tgagtcctgg gcaatcgttt ttttattact
     2401 tccgttaaat gttttgaaat tattctggga aaaccctatt tttccagttc aaatatggaa
     2461 tagtatgaaa atctcctcat tcctgctttt tgcagccact gtattgctcg ctcccacaag
     2521 gcttttggcc caggattgca atactttcat tgagaaagaa ctgacggtag gcggtttgca
     2581 tttgttgcgg accattccgc tcacaatggt gatacgcggc acctacactt acagcattca
     2641 aatcatcaac gacgaaaaag ggctgcgcgc caaagtcatc tctcgtgcag gagtggagtt
     2701 caatcaggac gatgagatta tttttatgga tgtgaactcg ttgcgcaaag gttatcgctt
     2761 tatggaaatg ggcgagatga aagaagaagg tggaatcccc gaataccaca acttcctgca
     2821 aatcaacttg gatgccatac agtggttctc cgaagtggac atcaatacca tttatattaa
     2881 aaacaatctg tcgaaggaaa tgcggaagtt tacggtgaac cccaaccgca tggccgattt
     2941 tcgcatgttg gcttcttgct tcgaccaacg cctcaacaaa accaatgtgg tcaatgtgac
     3001 gaatgccgaa cgggattaca ccaaaccggg cgccaatgat accggcttgt tgacggccaa
     3061 acctgaaacg cccactccgg gaaaacctgc tgctactccc gggaaacctg ctgtcaatac
     3121 tcccgctgcc gcatctggct cggacgccga attggacgct ttgcgccgcg atttggccgc
     3181 caccaaagag cagattcgca aagaaatagc cctcgaaaaa gaaaaggccc agcgatatcg
     3241 gcagcaattg caggacgaat tgtctttaga ccgtgaaaat gcggcccgcc aaaagaaaca
     3301 gtatgccgac gaggtgctcg atgcccgcca aaaagcgcag gcacaaatgg aggagtcgaa
     3361 acgcgcgctg gccgattcga ttcgattggc ccgtgccaat gccggttcgc aaattgaaaa
     3421 aatcaacctc gaagtacaag aatcccgcca gcgtgctgcc gaagccgtcc aaaaagcgca
     3481 gttggaatca gccaaagaag tagccgccgc tcgcgataaa gccgctgcgg aggtgaaaac
     3541 cctcaaggac aaaatggagc aggccaaatt gcaatacacc gacgaaattg cctcggcccg
     3601 cgaaaactcg aaccgggaat tggagcgcat ccggcaggaa tcttccgatc aggtgcggca
     3661 ggcccgcgaa gctgcacagc agcaatcaag cgccacgatg gagcaggtgg ccgaaagccg
     3721 cgaaactgca tcggcgctca ttttgcgcac caaagaagag gccgccgccg agatagcccg
     3781 cacgcgtgaa aatgctgtat tggaaaaaga acgcattgcc gttgaagtag cagaatcccg
     3841 cagaaaaggc gccgaggaaa tcgccctggc ccggcaaaac gcagccgctg aaaccgcggc
     3901 aattgccgaa cgcacctcga ccgaaaaaga ggctgccgcc cgcgaagtgg ccgaaacgcg
     3961 ccaacgcgcc gccgaacagg tggctcagat taaaaagcaa gtagctgaag aggaacaaaa
     4021 agggcagcaa caaaccgaag ccgcccggca ggaggccgca caatcggtgg cagaggcgcg
     4081 taggcaggct gccctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaaaatagc
     4141 agaggcgcgc caaaagagcg aggccgaacg caaaaccatt gccgatgaag tggctgccgc
     4201 tcgccaacgt gccttggacg aaattaaaaa aatacaggaa gaaagcgccc acgccatggc
     4261 cgaaacaaag gccgccgaaa ccaaaatgcg tgagcagtcg gcacaggagg tagctgctgc
     4321 ccgccaacgc gccgctgatg aagtgaaagc cgctcaggca aaagcagccg cacaggtagc
     4381 cgaaattgaa tccaagttgg aaaactcgcg gcaacaatac actcaggagg tgggcaacgc
     4441 tcaaaaaacg gctcaggaac gcttggctca aatacaaaaa gaagccgccg ccgctattgc
     4501 cgatgcaaag agcaaggaac aggaagccaa aaacacctcg gtagaggaag tggtggtgac
     4561 ccgtgaaaat gccgcgcgag aaattgcgaa ggctcgcgaa actgcggctt tggaaattca
     4621 aaaagcccgc gaacaggccg ttctggctca acaggagtcg gctaatgccg ctgccgccgc
     4681 ccgaaaatcc tcagcagacg ccgttgccca ggcccaacag gaggaatcac ggcagatcaa
     4741 agagttgaga gaaaaatctg ccgccgaaaa acaggcactg gccgccgaag tagctcag
//
LOCUS       DAORLF010000378         4733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9886_length_4733_cov_2.112869, whole genome shotgun sequence.
ACCESSION   DAORLF010000378 DAORLF010000000
VERSION     DAORLF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_4733_cov_2.112869"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>206)
                     /locus_tag="PK198_21890"
     CDS             complement(<1..>206)
                     /locus_tag="PK198_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41467.1"
                     /translation="TDAGVYYKDDYLSEWVEYGQLPHTRVEDIEIQYAANLLRIGTHG
                     RGMLEAPIEVAACTPFSPDDDADG"
     assembly_gap    207..378
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<379..2244)
                     /locus_tag="PK198_21895"
     CDS             complement(<379..2244)
                     /locus_tag="PK198_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41468.1"
                     /translation="MNRAIATLIITAFSLVLSGQSSLEEIFRSSRDFYKIVAEAEVYF
                     DRKHPGLTPWERTVGEHRDGEYVKFMRWRSFWEDRLNADGTLGDPTAHFIEKEKNTSR
                     PKTINPYENVSWTNISYLGYIDDQIGLGRTTSLAFHPTDPNTFYVGAAIGGVWKTTDG
                     GLSYVPLGDQLPFLAISSIIVDQQNPNTLYIAVSDHVWYGPQGIGVYKSTDGGQNWQP
                     TALSFNFTDDIRIYWLEADPTNPQRMFAATANGLYRTTDGFNSVSKVLNVSSFNVRLK
                     PGDPNIVYVGTRDGRVFRSLDGGGSFTQTADFGNNEVFLNVTPLSSRKVYARHGSDLY
                     KSTNEGATFSSGGVFPESGQVFVFSPANPSVILSGNFETFRSNDDGASFIQTMDWLGA
                     GGLVDIHVDQRNMFVNPLENHCVYYCNDGGVYRFILADNSFENLSDGLAITQFYDISV
                     SQSNAGIVGGGSQDNGNVFREANGVWADYAGTGDGMNQDIDPTDSRYRYWSYQNGAVR
                     RWFNGSNSNIEPPGHGNGAWETPYKLDPSNPARIVIAYTEVYESFNRGSNWNSISGAL
                     ANGSSLQQIAIAPSNGERIYAVHGDKIYVKNTADDSWTTRNLPANNISDLELDAAD"
     gene            complement(2428..3396)
                     /locus_tag="PK198_21900"
     CDS             complement(2428..3396)
                     /locus_tag="PK198_21900"
                     /inference="COORDINATES: protein motif:HMM:NF020756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AvaI/BsoBI family type II restriction
                     endonuclease"
                     /protein_id="HRF41469.1"
                     /translation="MAKKVGGHSLIESAEALITSREETRAGFIAMALEKNYMAVPYVE
                     QAIALKTLASSAESPLALLDMEMLRAGLLTASGLSEKSLNYLTEDDKTIAIKGLIENF
                     LEPAGTEFVDELVYRFLLTKGDALGGKARNLAGTLGERKFLRTLISVFNIAGISYRWK
                     DGDSNTWLEKPDDDAGIEKRIKALYWKGEHDRLLVLNVNVPMVNKNVDVIVLDAKEEE
                     YLKSSKDSIIYQAERYVALGEMKGGIDPAGADEHWKTANSALNRIRTGFNSLGFSPQL
                     FFIGAAIEAAMAQEIYQQLQNENLHQAANLTKDAQLTAICGWLAQI"
     gene            complement(3381..>3599)
                     /locus_tag="PK198_21905"
     CDS             complement(3381..>3599)
                     /locus_tag="PK198_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41470.1"
                     /translation="KSGGYCVMVNDNVRYGGEEIPVDLILSEFAEHFGFDIVKILVLP
                     KGKGNSSQQMGNYGRTEIRKCVYLWQKK"
     assembly_gap    3600..3651
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3652..>4733)
                     /locus_tag="PK198_21910"
     CDS             complement(<3652..>4733)
                     /locus_tag="PK198_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41471.1"
                     /translation="RKVVSFQANKEEPVYRWFKYKEGFSSVLVRHFLEEYGIRPGRLL
                     DPFAGAGTTLFAGQELGWESWGIELLPVGAFVMQAREAVSGMDVERFRNLVNQLWSDL
                     AVLKSDENHIRHIPITKDAFPEDTEELLNRYMSWCARIEDEKIRTLFRFAAFSVLEEI
                     SFTRKDGQYLRWDYRSARSLNGKPFDKGRILTFEEALKAKLQQITKDLAPEQILSLFD
                     DTEPAQKTATFQPINIIQGSCLEELPKLEANYFDFIVTSPPYCNRYDYTRTYALELVF
                     LGHDSDDVRNLRQAMLSCTVENKEKTEQLLQFYTSIGQADAFEKVIGVYEQSGAMREV
                     NAVLDLLNQQEKLNNSNIPRMVKNYF"
BASE COUNT         1112 a         1148 c         1041 g         1208 t
ORIGIN      
        1 atcccgtcag cgtcatcgtc gggtgaaaac ggcgtacatg ctgccacctc aatgggcgct
       61 tccaacatac cgcggccatg cgtgccgata cggagcaggt tggcagcgta ttgtatttca
      121 atatcttcca cacgggtatg cggcaattgg ccgtactcga cccattcgct gaggtagtcg
      181 tctttgtaat acacgccggc atcggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnat cggcggcatc caattccaga tcggagatat tgttggcggg
      421 gagattgcgg gtagtccagc tatcgtcagc agtatttttt acatagattt tatcgccgtg
      481 tacggcatat atcctctcgc cgttggaagg cgcaatggca atttgttgga gagaagagcc
      541 gttggccaag gcgccactga tgctgttcca gttgctgccc cgattgaaag attcatagac
      601 ctcggtgtaa gcgatgacta tgcgagcggg attggaagga tcgagtttgt agggtgtttc
      661 ccaggctccg ttaccgtgcc ccggaggctc tatattgcta ttggagccat tgaaccaacg
      721 ccggaccgct ccattctgat acgaccaata tctgtaccgg gaatcggtag ggtctatgtc
      781 ctgattcata ccgtcgccgg tgccggcata atcggcccaa acaccattcg cctcccgaaa
      841 cacatttcca ttatcctgag agccgccgcc gacaatgcct gcattggact gcgagacaga
      901 aatgtcgtaa aactgtgtaa tggccaagcc gtcggagagg ttttcaaagc tgttgtcggc
      961 caaaatgaag cgatacacgc cgccatcgtt gcaataatag acacaatgat tttcgagcgg
     1021 gttgacaaac atattccgtt gatcaacatg gatgtccacc aatccgccgg cgcccaacca
     1081 gtccatagtt tgaataaaac tggcgccgtc atcattggaa cggaaagttt caaaattgcc
     1141 ggaaaggata acagatggat tcgccggcga aaatacaaac acctggccgc tttcgggaaa
     1201 aacgcctcct gaagaaaaag tagccccctc attggttgac ttgtacaggt cggagccatg
     1261 ccgggcatat acttttctgg acgacagagg tgtcacgttc aaaaacacct cattgttgcc
     1321 aaaatcagca gtctgggtaa aactgccgcc cccgtccaaa ctcctaaaaa cccgtccgtc
     1381 tctggttccg acataaacga tattggggtc gcccggtttc agccttacat tgaacgaact
     1441 cacattcagc actttggaga cagaattaaa gccatcggtg gtgcgataca gcccgttggc
     1501 agtggccgca aacatacgct gcggattggt ggggtcagct tccaaccagt agatgcgtat
     1561 gtcgtcggta aagttaaaac tcagtgcggt aggctgccag ttttggccgc catcggtgct
     1621 tttgtacacg cctatgccct gcggcccata ccacacatgg tcgctcacgg cgatgtaaag
     1681 cgtgttggga ttttgctgat ctacgatgat ggaactgatg gccaaaaatg gaagctgatc
     1741 gcccagcggc acatacgaaa gccctccatc ggtagttttc cagacgccgc caatggctgc
     1801 accgacataa aatgtattgg ggtcggtggg atgaaatgcc agagaggtcg ttcttcctaa
     1861 gccgatctga tcgtcaatat atcctaagta ggagatgttg gtccaggaca cattttcgta
     1921 cgggttgatg gtcttgggac gactggtatt tttttctttt tcgatgaagt gcgctgttgg
     1981 atcccccagc gttccatcgg cattgagccg atcttcccag aaagaacgcc agcgcataaa
     2041 cttcacatat tcaccatccc tgtgctcgcc gaccgtcctt tcccagggtg ttaaaccggg
     2101 gtgcttccga tcgaaataga cctccgcttc agctactatt ttgtaaaaat cgcgggaaga
     2161 gcgaaatatc tcttccaaac tgctctggcc cgacaagacg agcgaaaagg cagtgatgat
     2221 aagcgttgct atggctctgt tcatgcgtgt gatgattgtg tagaggagat attcaagttg
     2281 ccggaataaa aaatctgcgc caaaattaac acaaacctct ccaaccacca ttttatcagg
     2341 aaaattgact gcagcctgac atgggaaaat gtgccgggca tgggttttta ggatttttat
     2401 tggggaagta atcccgataa atctgcatca tatttgtgcc aaccatccgc aaatagcagt
     2461 caattgcgca tccttagtca ggttggcggc ttgatgaaga ttttcgtttt gcagttgttg
     2521 atatatttcc tgcgccattg cagcttcgat ggctgcgccg ataaagaaca attgcggact
     2581 gaagccaagc gagttgaatc ctgtcctaat acggttgagt gcagaattgg cagttttcca
     2641 gtgctcgtct gcacctgcag ggtctattcc gcctttcatc tctccaagcg ccacataacg
     2701 ttcagcttgg taaataatgg aatctttact ggatttcaga tattcttctt cttttgcatc
     2761 caatactatt acatccacgt ttttattgac catcggcaca ttgacattca aaactaacag
     2821 ccgatcatgt tctcctttcc agtaaagcgc cttgatccgc ttttcaatac ctgcatcatc
     2881 gtcgggcttt tccagccacg tattggagtc gccgtctttc cagcgataag aaatcccggc
     2941 aatattgaaa accgaaatca gcgtgcgcag aaatttacgc tcgccgagcg tacctgccaa
     3001 atttcttgct tttccgccca gcgcatctcc tttggtaagc agaaaacgat acactaattc
     3061 atctacaaat tctgtccccg ccggttcgag aaaattttct atcaaaccct tgatagcgat
     3121 agttttatcg tcttcagtaa gataatttaa tgatttctcc gacaagccgg aagctgtcag
     3181 taatcctgcg cgcaacattt ccatatccaa aagagccaat ggactttcag cgcttgatgc
     3241 caatgttttg agcgctatcg cctgctctac ataaggaact gccatgtagt ttttttccaa
     3301 agccatcgct atgaagccgg ccctggtttc ttctctggag gtaatgagcg cttctgctga
     3361 ttcaatcagg gagtgacctc ctactttttt tgccataggt aaacgcattt tctgatttca
     3421 gtacgtccat aattccccat ctgctggctg ctgttacctt tgccttttgg aagcaccaat
     3481 atttttacga tgtcaaatcc aaaatgctct gcaaattccg acaggatcaa atccacagga
     3541 atttcttccc cgccgtaacg aacgttgtcg ttcaccatca cgcagtatcc gccgcttttn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaatagtt
     3661 cttcaccatt ctggggatgt tgctgttgtt caacttttcc tgctgattga gcaaatcgag
     3721 gacggcgttt acttcgcgca tagcgcccga ttgctcatat acgccgatca ctttttcgaa
     3781 ggcgtccgct tgtccgatgc tggtataaaa ttgaaggagt tgctctgttt tttctttatt
     3841 ttctaccgtg caagaaagca tggcctggcg cagattgcgc acatcgtcac tgtcatggcc
     3901 cagaaacacc agttccagcg cataagtacg ggtataatcg tacctgttgc aataaggtgg
     3961 agaagtaacg atgaagtcga aataattggc ttccaacttg ggtaattctt ctaagcagga
     4021 gccttgaatg atatttattg gttgaaacgt tgccgttttt tgtgccggct ccgtatcatc
     4081 gaaaagactg agtatctgct ccggcgcgag gtccttcgtt atctgttgca gcttggcttt
     4141 gagagcttct tcaaaagtca aaatccggcc tttatcaaat ggctttccat ttaatgacct
     4201 ggctgagcgg tagtcccaac gcaaatactg accatcttta cgcgtaaaac taatttcttc
     4261 caacaccgaa aatgctgcaa acctgaacag tgttcgtatt ttttcgtctt caatgcgcgc
     4321 gcaccagctc atgtagcggt tgagtagttc ttccgtatct tcagggaaag catctttcgt
     4381 tatcggaata tggcgaatgt ggttttcgtc agatttcaac accgccaaat cactccagag
     4441 ttggttcacc agattcctga aacgctctac atccatgccg ctcaccgctt ctctggcctg
     4501 catcacaaag gctcccaccg gcaacaactc aatgccccac gattcccagc caagctcctg
     4561 gcctgcaaat aaggtagtgc cggcacccgc aaaaggatcg agcaagcgcc cggggcgaat
     4621 tccgtactcc tccagaaaat gtctgaccaa taccgaggaa aagccttcct tgtacttgaa
     4681 ccagcgatac acaggctctt ctttgttggc ctgaaaactg actactttgc ggt
//
LOCUS       DAORLF010000379         4725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_9904_length_4725_cov_1.959529, whole genome shotgun sequence.
ACCESSION   DAORLF010000379 DAORLF010000000
VERSION     DAORLF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9904_length_4725_cov_1.959529"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK198_21915"
     CDS             complement(<1..823)
                     /locus_tag="PK198_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRF41472.1"
                     /translation="MKKVVLGFSGGLDTSYCAKYLSEDKGFEVHSIIVDTGGFSDEEL
                     AQIEQHAYRLGVATHTTVRAVRGYYERIIQYLIFGNVLKNNTYPLSVSAERLSQALHI
                     AEHARKLNADAVAHGSTGAGNDQVRFDMIFHIMIPHVEIITPIRDMRLSREEEVAYLH
                     SKGVEMNAAKAAYSINKGLWGTSVGGRETLQSNGFLPEEAWPTPVTQHEPRSLKISFR
                     QGHPVAVDDQTFEHPVEAIRHLQAIAAPYGIGRDIHVGDTIIGIKGRVGFEAAAPL"
     gene            complement(820..1518)
                     /locus_tag="PK198_21920"
     CDS             complement(820..1518)
                     /locus_tag="PK198_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF41473.1"
                     /translation="MAKQTKDKEQPPSGVSFIVEIASDQHTPYSQQICDEMEASAKAR
                     GTGIAKRSPDYVAQKMREGKAVIALADNGDWAGFCYIETWEHGMYVANSGLIVSPKFR
                     NSGLAKRIKHKIFELSRSRYPGSKIFGLTTGLAVMKINSELGYEPVTYSELTSDDEFW
                     AGCKSCVNYDILMSKERKNCLCTAMLYDPEDHYEPAETVEHFKEKKNVYERLLHIKQY
                     WLDKVFPKKQNGNK"
     gene            complement(1593..2759)
                     /locus_tag="PK198_21925"
     CDS             complement(1593..2759)
                     /locus_tag="PK198_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41474.1"
                     /translation="MSALTQLNTEARVRWIHRAVCLFFLIGIGMSLPLWAGERLFPHI
                     PVFSGLELPGAAHWGLAGLLAMALVLNMVQLKKWWAALLLGLMVLIVLPDQMRLQPWV
                     FVYFLILFPLGLVTLTEKNAAALLSYVQILLVGVYVWSGLHKFTQSFIDLVYPLLLKG
                     LFKLPEDNYLLEVREWGYLLAAVELSIGIGLIFPKTRNLAVIGAVFTHLLIIAWVLVG
                     NPNYTILPWNAAMIAVVILSSWNVKNRISLWPKTSPPLRWATVGLALLIWLMPVLNTK
                     NKWDAYLSFNLYTERISHLFVGMRQQALEGIDPKLNNYFAEENLIEEGRVIDVEKWSF
                     DELKVPVYPALRVHKAIGRDFCRPPADPNQIMLVTYRRPFREGHYEVFFCKDCE"
     gene            complement(2844..>3827)
                     /locus_tag="PK198_21930"
     CDS             complement(2844..>3827)
                     /locus_tag="PK198_21930"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF41475.1"
                     /translation="GEDRTVQTWLGSQSIETLRQNIGKHQLLVVDEAQHVPNIGLNLK
                     LIVDHLPQLRVLATGSSSFDLSNQTGEPLVGRKWEFELYPISQLELSATEAPFQTEEL
                     LPQRLIYGSYPEIVTTIGLSDKRDMLNNIVNGYLYKDLLMFEDIRKSKKIVEILSLLA
                     FQIGNVVSVHEISQAIGMNTRTVEKYLDLLEKVFVIKRLGGYSRNLRKEISKGSKYYF
                     WDNGIRNAIINNFNELPLRNDVGALWENYLIMERLKKQSYVKIFSNNYFWRTYDQKEV
                     DFVEERDGRLYGFEFKWSKTHTKPPALWLETYPEADFEVISQGNYLPFITA"
     assembly_gap    3828..3963
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4241..4351
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4462..>4725
                     /locus_tag="PK198_21935"
     CDS             4462..>4725
                     /locus_tag="PK198_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41476.1"
                     /translation="MEKFTHVLLWATASIFSPLQLKIMKKLYSICLFLVVCTALGAQA
                     PGSWTALGSGITAAPRDFLGIDAVSADVAWGLAEHPTFAVGARE"
BASE COUNT         1012 a         1120 c         1213 g         1133 t
ORIGIN      
        1 tgagcggcgc ggctgcctcg aagcccacgc ggcctttgat gccgatgatg gtatcgccga
       61 cgtggatatc gcggccaatg ccgtagggcg cggcgatggc ctgcaagtgc cggatggcct
      121 ccacggggtg ttcgaaagtc tggtcgtcaa cggcaacggg gtggccctgc cggaagctga
      181 ttttgaggct gcgcggctcg tgttgggtca cgggagtggg ccaggcctct tcgggcagga
      241 agccgttgga ttggagggtt tcgcggccgc ccacgctggt gccccacagg cccttgttga
      301 tggaataagc ggctttggct gcgttcattt ccacgccttt gctgtgcagg taggctacct
      361 cttcctcgcg gctgaggcgc atgtcgcgga tgggcgtgat gatctccaca tgtggaatca
      421 tgatgtgaaa aatcatgtcg aaacgcacct gatcgttgcc cgcgccggtg ctgccgtgcg
      481 ctacggcgtc ggcgttcaat tttcgggcat gttcggcaat gtggagtgcc tgactgagcc
      541 gttcggcgct gacactgagc ggataggtat tgtttttcag cacattgccg aaaatgaggt
      601 attggatgat gcgctcgtag tagccgcgca cggcccgcac ggtggtgtgt gtggccacgc
      661 cgaggcggta ggcgtgttgt tcgatctgcg ccagctcttc gtcgctgaat ccgccggtat
      721 ccacgatgat gctgtgaacc tcaaagcctt tgtcctcgct gaggtatttg gcacaatagg
      781 aggtatccag gcctccgctg aagccgagca ctactttttt catttgttgc cgttttgttt
      841 ttttggaaaa accttgtcca accagtactg cttgatgtgc agcaggcgct catacacgtt
      901 ctttttttct ttaaaatgct ccacggtttc ggccggttcg tagtggtctt caggatcgta
      961 gagcatggcg gtacagaggc agtttttgcg ctctttgctc atcagaatat cgtagttgac
     1021 gcagcttttg cagcccgccc agaactcgtc gtcggaggtg agttcggagt aggtgacggg
     1081 ttcgtagccg agttcggagt tgattttcat cacggccaag ccggtggtga ggccgaagat
     1141 tttggaaccc ggataccgcg agcgcgacag ttcgaatatt ttgtgtttga tgcgtttggc
     1201 cagaccgctg ttgcggaatt tcggcgaaac gatgaggccc gaattggcca catacatgcc
     1261 gtgctcccag gtttcgatgt aacaaaaacc tgcccaatcg ccgttgtcgg ccaatgcaat
     1321 gacggccttg ccctcgcgca ttttctgagc cacatagtcg gggctgcgct tggcgatacc
     1381 agtgccgcgg gccttggccg aggcctccat ttcgtcgcaa atttgttggg aatagggcgt
     1441 gtgctgatcg gaagcaattt ctacgatgaa cgagacgccc gagggaggct gctccttgtc
     1501 ttttgtctgt tttgccaact ttactgtact gtttttatgg taaaaaacgt gacaacatca
     1561 ccaaaagcgg cttggttgaa atgccttttc actcactcac aatccttgca aaaaaacacc
     1621 tcataatgcc cctctctgaa tggtcgtcgg taggtcacga gcatgatctg gttgggatcg
     1681 gcaggcgggc ggcaaaaatc cctgccgatg gctttgtgta cccgcagcgc cggatagacg
     1741 ggcactttca actcatcgaa cgaccatttt tccacgtcta tcacccggcc ctcttcgatg
     1801 aggttttcct ctgcgaaata gttgttcagt ttaggatcaa tgccctccaa agcctgctgc
     1861 ctcatgccca caaacagatg agaaatccgc tctgtgtaga ggttgaagga caaataggca
     1921 tcccacttgt ttttggtgtt caacacaggc atcagccaga tgagcagcgc cagacccacc
     1981 gtagcccaac gcagcggggg agatgttttg ggccacagac tgatgcggtt tttgacattc
     2041 caactgctca gtatcactac cgctatcatg gcggcgttcc agggcaggat ggtgtagttg
     2101 ggattgccga caagcaccca ggcgatgatg agcaggtggg taaaaactgc gccgatgacg
     2161 gccagatttc gtgttttggg aaagatcaac ccgatgccga tggacaattc aaccgccgcc
     2221 aaaagatagc cccactcgcg cacttcgagc aggtagttgt cttcggggag tttaaacaag
     2281 cccttgagta acaaagggta aacaaggtct atgaacgact gtgtgaactt gtgcagcccg
     2341 ctccatacat acacgccgac gagcagtatt tgcacatagc tgagcaaagc ggctgcattt
     2401 ttttcggtaa gcgtgaccaa gcccagagga aataaaatca gaaaataaac gaatacccag
     2461 ggttgcaggc gcatctgatc gggcaggacg atgaggacca tcagtccgag caaaagagcg
     2521 gcccaccatt tttttaattg caccatgttc agaaccaatg ccatggccaa aagccccgcc
     2581 agcccccaat gcgcggcgcc cggcaattcc aagcctgaaa aaaccggaat gtgtggaaac
     2641 aggcgctcgc cggcccacag cggcaaggac atgccgatgc cgatgagaaa aaacaggcaa
     2701 acggcacggt gtatccagcg tacgcgcgcc tcggtattca gttgtgtaag tgcagacatg
     2761 gcaaggtggt tttgagggga taaaaatagg gagtttggtg cattgttgct ccttcccgta
     2821 aattcataca aaggtggccc ccgttatgct gtaataaacg gaagataatt tccctgagaa
     2881 atgacttcaa aatcggcctc cgggtatgtt tcgagccaaa gtgcgggcgg tttagtatgc
     2941 gtttttgacc atttgaattc aaagccgtag agccggccgt ctcgctcttc cacgaaatcc
     3001 acttcctttt ggtcgtaagt acgccagaag taattgttgg aaaatatttt cacatagctc
     3061 tgctttttga ggcgttccat aatcagataa ttttcccaca aagccccgac atcattccgc
     3121 aaaggcaact cattgaaatt gttgatgatg gcattgcgaa tgccattgtc ccagaaatag
     3181 tacttcgaac ctttggaaat ttcctttcgg aggtttctcg aatagccgcc gaggcgcttg
     3241 atgacaaaga ccttttccag caggtcgagg tatttttcaa ccgttcgtgt gttcatgcct
     3301 atggcttggc tgatttcgtg taccgatacc acattgccga tttgaaaagc cagcaggctc
     3361 agtatttcta caattttttt ggatttcctg atgtcctcga acataagaag gtctttgtag
     3421 aggtagccat tgacaatgtt gttgagcata tcccgcttgt ctgacagtcc aatcgttgtg
     3481 acgatctccg ggtagctgcc ataaatcagg cgctgcggca acagttcctc tgtctgaaaa
     3541 ggcgcttccg tggcagagag ctcaagttgg gaaatcgggt agagttcaaa ctcccatttc
     3601 cttccgacaa gcggttcgcc ggtctgattg gaaagatcga aagaggaaga cccggttgcc
     3661 aatacccgga gctgaggcaa gtggtcaact atgagtttga ggttcaatcc gatattgggc
     3721 acatgctggg cttcgtccac aacgagcagt tgatgctttc cgatgttttg ccggagcgtt
     3781 tcgatgcttt gactgcccag ccaggtctgt acggtgcggt cttcgccnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnntgaataa ttcgctgaat tttcatatta tagtacataa aatttatcac aaaattatgt
     4021 attaggtagc aagattcata attctttcaa aaaaacagac cgcttggggc aaggcttcat
     4081 cttcatgcca acccggcaga gcgggaggca atattgggcc attttcggca cgcgatgatt
     4141 ccttttgcaa tgagtctaat ggtcattttg gcggaaatac accaattttt ggcggtttta
     4201 cacaaacacg ccggtggata aatgccgcaa ttttgcaacg nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacctggccg tgaccatcgg caggcgttgg
     4381 cgaagaagtt ggtgaccgcc gattcgccca cttttgacat gccgagcaac cactttttta
     4441 aaccttaaaa ctcgttgaat tatggaaaaa tttacccatg tattgctttg ggctacggcc
     4501 tccatctttt caccacttca actcaagatc atgaagaagt tgtactccat ttgcctgttt
     4561 ctcgttgtct gtaccgccct cggcgcacaa gcgcctggct cctggacggc cctcggttcg
     4621 ggcattaccg cagcgccgag ggattttctc ggcatagatg ccgtaagtgc ggatgtggct
     4681 tggggtcttg cagaacatcc cacgtttgca gtaggcgcca gagag
//
LOCUS       DAORLF010000380         4686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10022_length_4686_cov_1.988987, whole genome shotgun sequence.
ACCESSION   DAORLF010000380 DAORLF010000000
VERSION     DAORLF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_4686_cov_1.988987"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1055)
                     /locus_tag="PK198_21940"
     CDS             complement(84..1055)
                     /locus_tag="PK198_21940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF41477.1"
                     /translation="MKKALKIFGFLLAALALIIALIAAWVKLTPMPVYEVKPPQVVIP
                     TDSATLERGKKVAELVCAHCHLGEDGKMSGRLFSQATDPFGEMWTSNITQHPTKGAMS
                     RYSDGELAYLVRTGINREGRVVGYMMCHPQMSDEELGAVIAYLRSDAPMMQPSEVEHP
                     APEYLNSFLVKALIKFGVFKPLPYDGQPKSAPPMTDQVAYGRYLATDIYECGVCHSAS
                     FETFDLITPEKSPNFMGGSNPVPDHDFNLVASANITPSKDYGIGTWTKEQFSAAVRTG
                     QRPDGTILKPNMPRFAPLNEQEIDAIWAYLQTVPAIEQAAVLPTSSK"
     gene            complement(1084..1332)
                     /locus_tag="PK198_21945"
     CDS             complement(1084..1332)
                     /locus_tag="PK198_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41478.1"
                     /translation="MDGQTIDAPKGTLVVVPPMAVHTFKNALDAEAKFLIWFSPGGFE
                     QYFLDMEELVQNEPSWPPSDMGKVFALMARHDTYAPER"
     gene            1719..>3074
                     /locus_tag="PK198_21950"
     CDS             1719..>3074
                     /locus_tag="PK198_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41479.1"
                     /translation="MLKKHIIGTGFILCVLAGTGAQTVTLDSLRGVYLASKSDSAFWY
                     NSCLLGAVFFRSDLDSALLYSEISLDYARKAGKPNMIASSQLTIGAVYQYKDEREKAI
                     EWYLKAYETSKAGKQQRTEAVALGNIGLLYAELENTDKAVYYLKQSLAVMLQIKDTVG
                     IARSYANIGFNYFQQKSLDSAMVYFKKEVEWATLADDWNMQGAGLNNIGSVLLERNQL
                     AQARQYFLQSLEIREKNNDQQGLVNVLDNLGETLVKEGKVSAAIPYLERSMAIARQLG
                     GWANRINPAGHLANAYALTGEHRRAYEIQKELITAKDSVFQEEKQDRTDELEARFRNQ
                     LQAAELAQKQLDLERQTGQKRLILFGALAVVLGLVALFQFFRSRLRLRRKEAELAAQL
                     EHAEAENLRELNSVKSAFFANISHEFRTPLTLILGPLEQMMAGAFKGDFQKYYRIMHR
                     NG"
     assembly_gap    3075..3192
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3193..>4023
                     /locus_tag="PK198_21955"
     CDS             <3193..>4023
                     /locus_tag="PK198_21955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF41480.1"
                     /translation="VAQSFESLADKKLIQLQITLPESPVSGFFDRDKVEKIVANLVSN
                     AFKFTPDNGRIHISVAPDASIAVQDTGIGIPAEQIPHLFERFYHSSSSDIQSGSGLGL
                     ALTKELAELHGGGIEVESKEEQGTTFVVKIRTDEAFFQPGEIVETPQQSGIETQMPLP
                     AAAKPAPSSTRLALQANQPVLLIVEDNADVRAYIKDQFEGSYQIWEAENGRIGLEMAQ
                     KKTPDLIISDVMMPEMSGMELCSRLKTDEKTSHIPVIMLTARAEQTDKVEGLQTGADD
                     Y"
     assembly_gap    4024..4045
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4046..4487
                     /locus_tag="PK198_21960"
     CDS             <4046..4487
                     /locus_tag="PK198_21960"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF41481.1"
                     /translation="PKPFDALELTVRVANLIEQRRRLQAHYRQALSPFAPAAVEVPGM
                     DAVFLSRVREVVEANLEDENFSVVELGAQIGMSRSQLHRKLSALTGFSPNEVIRNMRL
                     ERAKQLLEKKAATVSEAAYGCGFSSPGYFSKCFKDYFGVLPGEV"
     gene            complement(4500..>4686)
                     /locus_tag="PK198_21965"
     CDS             complement(4500..>4686)
                     /locus_tag="PK198_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF41482.1"
                     /translation="QDMLVVKSIVSRNQKHPNTAHRQAALKAAQHIAQILDIQELPRD
                     PVAFLRTLLRDYIVLTR"
BASE COUNT         1102 a         1174 c         1245 g         1025 t
ORIGIN      
        1 gctaccggcc cggaaagcag ggcaatgagg cccgctgtct tgatgaatgt ggtttctgtt
       61 ttcataatag ttggtggttg tgattatttt gaagatgtag gtaaaactgc cgcctgctct
      121 atcgccggca cggtttgcag ataggcccaa atggcgtcta tttcctgctc attgaggggg
      181 gcgaacctcg gcatgttcgg cttgagaatt gtgccatcgg ggcgctgacc agtgcggacg
      241 gcggctgaaa actgctcttt ggtccaggtg ccgatgccgt agtctttgct gggcgtgatg
      301 ttggcggaag ccaccagatt gaaatcgtgg tcgggtacgg gattactacc ccccatgaaa
      361 ttgggtgatt tttccggggt aatcaagtca aaggtttcaa agctggcaga atggcatacg
      421 ccgcactcgt aaatatcggt ggccaaatac cggccataag ccacctggtc ggtcatagga
      481 ggcgccgatt tgggctgacc atcgtagggc agcggtttga atacgccgaa cttgatgagg
      541 gccttgacca aaaaagaatt cagatattca ggtgcagggt gctccacttc ggaaggctgc
      601 atcatgggcg cgtctgaacg gaggtatgca atgacggcgc cgagttcttc gtccgacatc
      661 tggggatgac acatcatgta gcccaccacg cggccctcgc ggttgatgcc ggttcggacg
      721 aggtaagcca gttctccgtc agaatagcgg ctcatggcgc ctttagtggg gtgttgggtg
      781 atgttgctcg tccacatttc gccaaaaggg tcggtagcct gactgaacag ccgtccgctc
      841 attttgccgt cctcacccag atggcagtgg gcgcatacca attcggccac ttttttgccc
      901 cgctccaggg tggcggaatc ggtgggtatc actacctgtg ggggctttac ttcgtacacg
      961 ggcatgggcg tgagctttac ccaggcagct ataagtgcaa taatgagcgc aagcgctgcg
     1021 aggaggaatc cgaaaatttt gagagccttt ttcataagaa tagaatttta aggtgaaaga
     1081 cttttatcgt tcgggagcat aagtgtcgtg ccgggccatc agtgcgaaga ccttgcccat
     1141 gtcggagggc ggccaggaag gctcgttttg cacgagttcc tccatatcga gaaaatactg
     1201 ctcgaaaccg ccggggctaa accagatgag gaactttgct tcagcatcga gtgcattttt
     1261 gaacgtgtgg acggccatag gcggcactac caccaaagtg cccttgggcg catcaatggt
     1321 ttgcccgtcc aatgtgaatg gagcgcgcct tcaagcacat aaaatacctc ctgcattttt
     1381 ttgtggtagt gtggcggtgg gctgatggga gagggcggca ggtggtattc gatcatggac
     1441 cacaggccat tggtttgttc tgtcttcgct ttgaagtaaa tgttcacgcc gtaggcgctc
     1501 aacacaggcc cctgatccgg cagcgacaca ataggatgta atgtgttcat aattctaatt
     1561 ggcattggtg actaagggaa agcatgagtt cgctttcaaa acacgatgca aaattggccc
     1621 ctggcgaggc ggaggggtta gcaattttgt cgcaaagact tgcagatttg tcgcaaatgc
     1681 ctaatttaga ggcagttttc acctaatgcc tcctcgccat gctgaaaaaa cacatcatcg
     1741 gaacgggatt tatcctctgc gttcttgccg gaaccggcgc acaaacagtc acgctggaca
     1801 gcctgcgcgg ggtgtacctg gcttcaaaat ccgactcggc attttggtac aattcctgcc
     1861 tgctgggggc ggtatttttt agaagcgact tggattcggc cttgctgtat tccgagattt
     1921 ctttggatta tgcccgaaaa gccggcaagc ccaatatgat cgcctcctcc cagctcacca
     1981 tcggagccgt ttaccagtac aaagacgagc gcgaaaaagc catagaatgg tatctcaagg
     2041 cttatgaaac cagtaaagcg gggaaacaac aacgcaccga agcagtggca ctgggcaata
     2101 tcggcctgct gtatgcagaa ctggaaaata ccgacaaagc cgtatattac ctcaaacagt
     2161 ctttggcggt catgcttcaa atcaaagaca ccgtcggtat tgcacgatct tatgccaaca
     2221 tcgggttcaa ttattttcag caaaaatcgc tcgactcggc tatggtatat ttcaaaaaag
     2281 aagtggaatg ggccacgctg gccgacgact ggaacatgca gggcgcgggg ttgaacaata
     2341 tcggctcggt acttctggag cgcaaccaac tcgctcaggc ccggcaatat ttcctccaat
     2401 cgctcgaaat tcgtgaaaaa aacaacgacc aacagggctt ggtgaatgta ctcgacaatt
     2461 tgggtgaaac cctcgtaaaa gaaggcaaag tgagcgcagc cattccttac ctcgagcgca
     2521 gcatggccat tgcgcgccaa ctgggcggct gggccaatcg catcaaccct gccgggcacc
     2581 tggccaatgc ttatgccctg acaggcgagc accgccgggc ttacgaaata caaaaagaac
     2641 tcatcacggc caaagattct gtttttcagg aagagaaaca ggatcgcacc gacgagttgg
     2701 aggcccgctt tcgcaaccag cttcaggccg ccgagttggc acagaaacag ttggacctcg
     2761 aacggcagac tggccagaaa aggctgattc tattcggtgc tttggccgtg gttttaggac
     2821 ttgtcgcgct gttccaattt ttccgaagcc gcctgcgcct ccgcagaaaa gaagccgaat
     2881 tggccgccca actcgaacat gccgaagccg aaaatctgcg cgaactcaac agcgtgaagt
     2941 ctgctttttt tgccaatatc agccatgaat ttcgcacgcc gctcaccctc attctcggcc
     3001 cgttggagca aatgatggct ggcgctttca aaggagattt tcaaaaatat tatcgcatca
     3061 tgcaccgcaa cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nngtggccca gtcgtttgag agtctggccg ataaaaagct gatacaattg
     3241 cagataaccc tgcccgaatc gcccgtcagt ggatttttcg accgggataa ggtggagaaa
     3301 attgtggcca atctcgtttc caatgccttc aaatttaccc ccgacaatgg ccggattcac
     3361 atatccgtcg cccccgatgc ctctattgct gtacaagaca ccggcatagg cattccggca
     3421 gaacaaatcc cacacctgtt cgagcgtttt taccattctt cttcctccga cattcaaagc
     3481 ggcagcggcc tcggtctggc gctcaccaaa gagctggcag aactacatgg cggaggcatc
     3541 gaggtggaaa gcaaagaaga acagggaacg acgtttgtag taaaaatcag aaccgacgag
     3601 gcgttcttcc aacccggcga aatcgtggaa actccgcaac aatcaggtat agaaactcaa
     3661 atgccgttgc ctgccgccgc caagcctgcg ccgtcttcaa cccgacttgc gctacaagcc
     3721 aaccaacccg tcctgctcat cgtggaagac aatgccgatg tgcgcgctta catcaaggat
     3781 cagtttgagg gatcgtacca gatttgggag gccgaaaacg ggcgcatcgg gctggaaatg
     3841 gctcagaaaa aaacgcccga tctcattatt tcagatgtta tgatgcccga aatgagcggc
     3901 atggagcttt gcagcaggct caaaaccgat gaaaaaacca gccacattcc cgtcatcatg
     3961 ctcactgccc gcgcagagca gaccgacaaa gtggaggggt tgcaaactgg cgccgacgac
     4021 tacnnnnnnn nnnnnnnnnn nnnnngccca aaccgttcga tgccttagaa ctcaccgtac
     4081 gcgtggccaa cctcatcgag cagcggcgca gacttcaggc gcactaccgc caggcgcttt
     4141 ctccttttgc ccccgcagcg gtagaggtcc ccggtatgga tgctgtgttt ttgagccgcg
     4201 tgcgcgaagt ggtggaggcc aatctggaag atgaaaattt cagcgtggta gaactcggcg
     4261 cacaaatcgg aatgagccgc agccaattgc atcgcaaact cagcgcactc acggggtttt
     4321 cgcccaatga agtcatccga aatatgcgct tggagcgggc gaaacaactc ctcgaaaaaa
     4381 aggcggctac cgtttcagaa gcggcttatg gctgcggatt cagcagtccg gggtactttt
     4441 ccaagtgttt caaagactac tttggggtac tgcccggcga ggtctgaggt ccagttccgt
     4501 cacctcgtca gcacaatata atctctgagc agggtgcgca aaaaggccac cggatcgcgg
     4561 gggagttcct gtatgtcaag tatttgggca atgtgttgtg ctgctttgag ggccgcctgt
     4621 cggtgggcgg tgttggggtg tttttggttg cgcgacacaa tactcttcac taccagcatg
     4681 tcctgt
//
LOCUS       DAORLF010000381         4679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10040_length_4679_cov_1.842128, whole genome shotgun sequence.
ACCESSION   DAORLF010000381 DAORLF010000000
VERSION     DAORLF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_4679_cov_1.842128"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..967
                     /locus_tag="PK198_21970"
     CDS             218..967
                     /locus_tag="PK198_21970"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF41483.1"
                     /translation="MAWKWYDSTIVRIEDCAPDVRRFWLQMPVDDPLEFKAGQFVTMD
                     LPIHEKRLKRWRSYSVAGPPGEKEMPEFCIARLEGGAASAYFFETAQVGETIRFKGPD
                     GNFVLPDELSDRDLVFICTGTGIAPFRSMIQDIRRRQLPYRSIHLIFGTRYAAGILYR
                     HEWEALLRDMPDFQFSVALSRESELRAEDFSFPVYKGYVHQIYMDAYPKAQPGQDFYI
                     CGWSNMIDDAVENLLVKMAADKSRIHYELYG"
     gene            1054..>2484
                     /locus_tag="PK198_21975"
     CDS             1054..>2484
                     /locus_tag="PK198_21975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41484.1"
                     /translation="MKRILFVFVAMLASWAIQAQDSRLAQQYFQNGEYEKAAVLFEQL
                     YDANPQNDYFFDRFVDCLISLERYDACEKAIRKQLKRDPKNLPLYVTLGRLFEQQYRD
                     ADAEQQYKEAIAQLPKEHFAIIRLANAFTNMTKYDYAIKTYEKGSEILKDNVSFSFYL
                     GDLYRRKGDTPKMIDSYLNSLATTPDRLPTLQTTFQRFFTDEDYKELQKQLYVRLQQD
                     ENSIFYTEMLAWSLMQQGDYRNALRQLRALDKRLRENGGRVFQLAQIAAGARDYDAAI
                     AAYEYIVEEKGRSSTFYLEAKREALRCRRSKLTEGYAYTREELLVLQQQYESFLSEFG
                     RSRVTAGIISEQAELEALYINDLDKAIGLLTEVIEFPNADPILVANAKLNLGDYYLMQ
                     GEVWEATLLYSQVDKAYKEDALGNEARYRNARLSYYDGDFQWAQAQFDILKASTSKFI
                     SNDAIDLSVFIMDNLGLDTTAEALTLY"
     assembly_gap    2485..2553
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2554..2867
                     /locus_tag="PK198_21980"
     CDS             <2554..2867
                     /locus_tag="PK198_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41485.1"
                     /translation="SAIVNGQVLLTLEDDVLYLKSKIYNKKRDYIKQAETLQRIMEGY
                     PEEIRADNALFELAELYQTHLNDPEKAKELYEKLFIDYSSSTFAVEARKRFRIMRGDK
                     V"
     gene            complement(2878..3960)
                     /locus_tag="PK198_21985"
     CDS             complement(2878..3960)
                     /locus_tag="PK198_21985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRF41486.1"
                     /translation="MAHNKTHQEIRAAVAFFLPGCTVSGIKGHGTGHIHQTYRVSTDA
                     ARSSGRFLIQQFNHQVFKSPEAVAANIALVHAHLATQQLDTVILHPVSDPNEQYFYIA
                     ESGRLWRMFDFIDGGYSTERAESAQQAYLAGKAFGAFATALSDLDASLLAETIPGFHD
                     SVSRWRDFEGVVNTDPARRVQEAMPLIEALKRHHHIFFELPSLPVPVRIVHNDAKLGN
                     VLFEQTSHRILAVTDWDTIMPGCILADFGDLARSLISPVEEDSPRYDKIQVQLPYFEQ
                     LCRGFLEDSSAILTTIEKERLVLGAKWIILEQALRFLSDYLSGDVYYPVCYSEHNKTR
                     TINQLRLFEEILAHEAAMQAIVEKYA"
     gene            complement(4066..>4679)
                     /locus_tag="PK198_21990"
     CDS             complement(4066..>4679)
                     /locus_tag="PK198_21990"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF41487.1"
                     /translation="ASEVGVFLGASNYMGEFAQSPISFNETNAAFGLVYQRFIDPQWG
                     VKGSLTFGKISGSDRNLDPRILKDRDWSFQANITEVAGNLQFHPWGKARFNQVGQLQK
                     HFSPYASLGLGLAFANAGLDVPADDLVRSREPDNRSVFFSVPVAAGIRYVMTEKLTLN
                     LEYGQRATFSDYLDNVSRNGNPDRNDWYMFLGFGLTYSLMAEY"
BASE COUNT         1144 a         1225 c         1215 g         1026 t
ORIGIN      
        1 acgcttgatg agtggattta cttcctgaaa acggctgaaa tcaaagacga atttaccgag
       61 aaaggattga aagccggccg gtaagcgctc acagacatcg cacctgacag cccgcgttac
      121 ggccccgcat gaacaaattc agccctcgcg ccgtattatc ctcatacctg ctgccgaccg
      181 gcgcccgtcc gaaacgcagc gcaacaaccg ctacactatg gcctggaaat ggtacgacag
      241 caccatcgtt cgcatcgaag actgcgcgcc cgatgtgcga cgattttggt tgcaaatgcc
      301 tgtggacgat ccgcttgaat tcaaagccgg gcagtttgtt accatggact tgcccatcca
      361 cgagaagcgg ctcaagcgct ggcgcagcta ctcggtggcc ggtcctccgg gagaaaaaga
      421 aatgccggag ttttgcatcg cccgattgga aggcggcgcg gcctcggctt atttttttga
      481 aacggcgcaa gtgggcgaaa ccatccgatt caaaggcccc gacggcaatt ttgtactccc
      541 cgacgagctc tccgaccgcg atctggtctt tatctgcacc ggcaccggca ttgctccgtt
      601 tcgcagcatg atacaagaca tcaggcgccg ccaattgccg taccgaagca tccatctcat
      661 cttcggcacg cgctacgccg ccggcatttt gtaccgccat gagtgggagg cgctcctgcg
      721 cgatatgcct gattttcagt tctctgtggc gctttcgcgg gagagcgaac tgcgcgctga
      781 agatttcagc tttccggtgt acaaaggcta tgtgcatcag atttatatgg acgcctaccc
      841 gaaggcgcag cccggccagg acttctacat ctgcggctgg agcaatatga tagacgacgc
      901 cgttgagaac ctgctggtaa aaatggccgc cgacaaaagc cgcattcatt acgagttgta
      961 cggctgaatc aggagaatgg ccccttttga tacacgaggg caagcaactt ttgccctctc
     1021 ccggcgtttt ttgggagcaa tacctatgtg actatgaaac gtattttgtt tgtattcgtg
     1081 gccatgctgg ccagttgggc tattcaggcg caagattccc ggctggcgca gcagtatttc
     1141 cagaacggag agtatgaaaa agcagccgtt cttttcgagc agttgtacga tgccaacccg
     1201 caaaatgact atttttttga tcgttttgtg gactgcctca tttcgctgga gcgctacgat
     1261 gcctgtgaaa aagccatccg aaagcagctc aaacgcgacc ccaaaaacct gccgctgtac
     1321 gtcacgctgg ggcgcctgtt cgagcagcaa taccgcgacg ccgatgccga gcagcaatac
     1381 aaagaagcca ttgcgcagtt gcccaaagag cattttgcca tcatccgctt ggccaatgct
     1441 ttcaccaata tgaccaaata cgactacgca atcaagactt acgaaaaagg cagcgagatt
     1501 ctgaaagaca acgtttcgtt cagtttttat ctcggcgacc tctaccgccg caaaggcgat
     1561 acccccaaaa tgattgacag ctacctcaac agcctggcca caacgcccga ccgactgccg
     1621 accctgcaaa ccacgtttca gcgctttttt acagacgagg attataaaga actgcaaaag
     1681 caactctacg tccgccttca acaggacgaa aactccattt tttacaccga aatgctggcc
     1741 tggtcgctga tgcaacaagg cgactaccgc aatgcgctgc gccagttgcg cgctttggac
     1801 aaacgcctgc gcgaaaacgg aggccgcgtg ttccagttgg cacaaatagc cgccggcgcc
     1861 agagattacg acgccgccat agccgcttat gaatacatcg tggaagaaaa aggaagaagt
     1921 tccacttttt accttgaagc caagcgggag gccctgcgtt gccgtcgcag caaactcacc
     1981 gaggggtacg cctacacccg cgaggaactg ctggtgttgc aacaacaata cgagagcttt
     2041 ttgtcggagt tcgggcgttc ccgcgtcacg gccggcatca tttccgaaca ggcagagttg
     2101 gaggcattgt atatcaatga cttagataaa gccatcggcc tgctcaccga ggtgattgag
     2161 tttccgaatg ccgaccccat tctcgtggcc aatgccaagc tcaatctcgg cgattactac
     2221 ctgatgcagg gcgaggtttg ggaggctacc ctgctttatt ctcaggtgga caaagcctac
     2281 aaagaagacg cactgggcaa cgaggcccgc taccgcaatg cccgcctgtc ttactacgac
     2341 ggcgattttc agtgggcgca ggcgcagttc gacattctca aagcctctac ctccaagttc
     2401 atttccaacg atgccattga cttgtcggtg ttcatcatgg acaatctggg cttagacacc
     2461 accgcagagg cgcttaccct ttacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctccgc aatagtgaac gggcaagtcc
     2581 ttctcacgct cgaagacgat gtgttgtacc tgaagtcgaa gatttacaac aaaaaaagag
     2641 actatatcaa gcaggctgaa acactccaac gcatcatgga gggctatccc gaagaaatca
     2701 gggccgacaa tgcgctgttt gaattggcgg aattgtacca aacacacctc aacgatcctg
     2761 agaaagccaa agagttgtac gagaagttat tcattgatta ctccagttcc acctttgccg
     2821 tagaggcgcg caagaggttc cggatcatgc gcggcgataa ggtgtaaaac acggctttca
     2881 ggcatatttt tcgacgatgg cctgcatagc ggcttcatgg gccagtattt cctcgaataa
     2941 acgcaactga ttgatagtgc gcgttttatt gtgttccgaa tagcagacgg ggtaataaac
     3001 atctcccgac aggtagtcgc tgagaaaccg cagagcttgt tccaatatga tccatttggc
     3061 gcccagtact aaacgttctt tttcgatggt cgtaaggatg gcagagctgt cttccagaaa
     3121 gccccggcag agttgttcaa agtaaggaag ctgaacctgt attttatcat acctcggact
     3181 gtcttcctct accgggctga tgaggctgcg cgccaggtcg ccgaaatccg ccagaatgca
     3241 tcccggcatg atggtgtccc aatcggtgac cgccagaatg cgatggcttg tttgttcaaa
     3301 aagcacattg cccaatttgg cgtcgttgtg aacaatgcgc accggaacag gcagcgacgg
     3361 cagctcaaaa aatatatgat ggtgtcgttt caaggcttct atcaggggca tggcctcctg
     3421 tacgcgcctg gcaggatcgg tgtttaccac gccctcaaaa tctcgccatc tgctcacgct
     3481 gtcgtgaaag ccgggaatgg tttcggccaa aaggcttgca tccaaatcgc tgagtgcagt
     3541 ggcaaaggcg ccaaatgcct tgcccgccag atacgcctgt tgagccgatt cggcccgttc
     3601 tgtggagtag ccgccgtcta taaagtcgaa catgcgccag agccggccac tttcggcgat
     3661 gtaaaaatac tgctcattcg ggtcggaaac ggggtgtagg atgacggtat caagttgctg
     3721 agttgcgagg tgtgcgtgta cgagggcaat attggccgct actgcttccg gggatttaaa
     3781 aacctgatga ttaaactgtt gtatcaaaaa acgccccgaa gacctggccg cgtcggtgct
     3841 tacgcgatag gtttgatgga tatgcccggt accatgccct ttgatacccg acacggtgca
     3901 gccgggaaga aagaaggcga cggctgcccg aatttcctga tgcgtcttgt tatgtgccat
     3961 aaaaaaaaat gtgcttcgcc tctgcctttg tatgaatggg cagaaacgaa gcacacaaaa
     4021 tacgttcaaa ttgtgatctg atttgtcgaa gcggataatt ttttgtcaat actcagccat
     4081 caacgaataa gtaaggccaa agccgaggaa catgtaccag tcgttgcggt cggggttgcc
     4141 gttgcggctc acattgtcca aataatcgga gaaagtggcg cgttggccat attcgagatt
     4201 cagcgtcagc ttttcagtca tcacatagcg gatgccggcg gcaacgggga cggagaagaa
     4261 aacgctgcgg ttgtcaggct cgcgggaacg cacgaggtca tcggccggaa cgtctaatcc
     4321 ggcattggca aaagccaggc ccaagcccag agatgcgtac ggagaaaagt gtttttgcaa
     4381 ttggcccact tggttgaatc tggctttgcc ccaggggtgg aattgcaaat tgccggctac
     4441 ctcggtaatg tttgcctgaa aagaccagtc gcggtcttta agaatgcggg gatccagatt
     4501 gcggtcgctg cccgaaatct taccgaaagt gagggagcct tttacgcccc actgcgggtc
     4561 gataaagcgt tgataaacca agccgaaggc ggcattggtt tcgttgaagg aaatcgggct
     4621 ttgggcaaat tcgcccatgt aattggaagc ccccaaaaac acgccgactt cggaagcgc
//
LOCUS       DAORLF010000382         4676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10045_length_4676_cov_2.329799, whole genome shotgun sequence.
ACCESSION   DAORLF010000382 DAORLF010000000
VERSION     DAORLF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_4676_cov_2.329799"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..766)
                     /locus_tag="PK198_21995"
     CDS             complement(29..766)
                     /locus_tag="PK198_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF41488.1"
                     /translation="MTEFDNFFKSAFTVDNVIFGFDEGDLKVLLIKRGEAPYMGQWAL
                     PGYFVYPNEDLDTAANRVLEELTGLRHVYLEQVKTFGAVNRHPFGRVITVAYFSLVKI
                     NDYKLQPASIARKAQWHPVSEVKELAFDHFEILQSCFQRLKSSVRWRPIGFELLPPHF
                     TLTELQHLYEAILETELDKRNFRKKILGMDLLLDLDQTQEGVAHRPAKLYRFDREKYL
                     RFQAEGFNFELKEGQRKEVRKRRMEAV"
     gene            906..>1314
                     /locus_tag="PK198_22000"
     CDS             906..>1314
                     /locus_tag="PK198_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41489.1"
                     /translation="MEIKLLNWREIPMVRLCAALILGIIAGAWLPGTGLWIVAAAGVA
                     GLAGMLFIGARRQAYEQRWIFGALLYLSLSALGYVLVGINDERNVDNHFSNRLLEENI
                     VAAEVEQVQLRNERFRMTVKVGAIGRSAGELQPV"
     assembly_gap    1315..1365
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1366..>1667
                     /locus_tag="PK198_22005"
     CDS             <1366..>1667
                     /locus_tag="PK198_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41490.1"
                     /translation="PGSHLVFSASITPIQAPLNPKAFDYARYQHYKNVHYQTFVGEDA
                     WQLSDRPASFSLSKYTDRARRYCLAVLRKHLPTPNEFGAASPLILGYRDETPDALR"
     assembly_gap    1668..1690
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1691..3026
                     /locus_tag="PK198_22010"
     CDS             <1691..3026
                     /locus_tag="PK198_22010"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRF41491.1"
                     /translation="THVLAVSGLHVGIVQMVISFLLQFWGRKSGRHRIPKMLITILGV
                     WAFALITGGAGSALRAATMFSFLTAGQAIRQSSNTYNTMAASLFLLLCINPLLLFDLG
                     VQLSYLAVLGIVFFHPRIYRLWYIENKAGDYLWQLTALSLAATLTTLPLSLLYFHQFP
                     VYFWLSGLVAVPLSGFILGGGLLLFAVQGIPVLGFAVGKALYALVWLMNAAIFLIQQL
                     PGSRISGIWISVAVAVLLYGVIIAVAGGLVTKKFRWILAALTLGVLAAGINTFEVWQA
                     HQRREIVLYHTRRQTIIDCFDGRQVFSLSANPVTVEEERFALQNYRWYARSRMAGAWL
                     LSDAPPTAERWWYRKGLLRLGDVRLAVVSEPQAPLSEKIPTDYLLLRGRPYASLEELA
                     EAWDIRSRTILLDGSLSPRQAARLMEEGRELGYQCYDVSNRGAWILNLHKTQ"
     gene            3038..4162
                     /locus_tag="PK198_22015"
     CDS             3038..4162
                     /locus_tag="PK198_22015"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF41492.1"
                     /translation="MKPLKDFFHYTRSERNGALILLLLCIILMVAPRLFVLFPSSERI
                     DFEEEMRLAEQLAQSSREEDGTPGTPAQLFEFDPNTATQEELQRLGLRERTVKSIVNY
                     RSKGGQFRKKEDLKKIYTLAEEDYFRLEPYIRIASNDSYSNYNSYDKKEPKTYTLVAF
                     DPNTATQEELLNLGLPKGVVTVMLRFREKGGKYRRKEDLKKIYILDEADYLRIEPYIS
                     IAGDGASIPTIALNIPQSYENERNISIDINKASAEEWQQLRGVGPAFSGKITRFREAL
                     GGFTSIEQVAETRGLPDSTFQKIRSQLRPSPVFRLIDVNAAPADVLSKHPYVDRRTAE
                     AIVSYRQQHGAFRSADDLRKVYALPEGWVEKMKPYLDFGK"
     gene            4194..4649
                     /locus_tag="PK198_22020"
     CDS             4194..4649
                     /locus_tag="PK198_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41493.1"
                     /translation="MRIAITLLAFAAMFSMVACKQDAKTGAGTSPDPAATAVTGVVHH
                     YICPNNCAGSGGEAAGTCPTCGTEYLHNDAFHNQPGQQPAPAPTPQVQTSPSVATPSI
                     TPPPSTDPPQNAAGVWHYTCAKGCAGGSGAVGKCATCGGDLAHNAAYHQ"
BASE COUNT         1045 a         1209 c         1239 g         1109 t
ORIGIN      
        1 aagaacgcaa gaactttttg gctcaacctc acaccgcctc catcctcctc ttccgcacct
       61 ctttgcgctg gccctctttc agttcaaaat tgaagccctc ggcctgaaag cggaggtact
      121 tttcgcggtc gaagcgatag agcttggccg ggcggtgcgc aacgccttcc tgagtttggt
      181 ccaagtctaa gagcaagtcc atgccgagga tttttttgcg gaagttgcgt ttgtccaatt
      241 ccgtttctaa gatggcttca tagaggtgtt gcagttcagt gagggtgaaa tgcggcggca
      301 gcaactcgaa tccgatgggc cgccaacgca ccgagctttt gaggcgttgg aaacagcttt
      361 gcaaaatttc aaagtgatcg aatgccaatt ctttcacttc tgataccgga tgccactgcg
      421 ctttgcgggc aatggaggcc ggctgaagtt tgtagtcgtt gattttgacc aatgaaaaat
      481 aagcaaccgt gatgaccctg ccgaaagggt ggcgattcac ggcgccgaag gttttaactt
      541 gttccagata gacgtggcgc aggccggtca gttcttccaa tacccgattg gcagcagtgt
      601 caaggtcttc attgggatac acaaaatatc caggcaaagc ccattgcccc atatacggcg
      661 cttcgccgcg cttgatgagc agtactttca ggtcgccttc gtcaaaaccg aagatcacat
      721 tgtcaacagt aaaggccgat ttgaaaaaat tgtcgaattc tgtcattgtt tgttcaatag
      781 tttgatgtaa aattgaggcg tgtaataaaa gaacagccga aattagtgtg tattttacac
      841 cagtgcaatt ttttgtgcaa aattgatatt tgcagatgat acttggttaa aataagacca
      901 aaaaaatgga gataaaactg ctcaactggc gcgaaatccc catggtgcgg ctatgtgcgg
      961 cgctcatcct cggcattatt gccggggcgt ggctgccggg aacaggttta tggatagtgg
     1021 cggcggcggg cgtggccggg ttggcgggta tgttgttcat cggcgcccgg cggcaggctt
     1081 atgagcagcg gtggattttc ggggctttgt tgtacctgag tttgtcggcc ttgggttatg
     1141 tactcgttgg gataaacgat gagcgcaatg tagataatca tttttccaat cgcctgctgg
     1201 aagaaaatat cgtagcggca gaggtggaac aggtgcaatt gcgcaacgaa cgcttccgaa
     1261 tgaccgtaaa agtgggcgcc ataggcagaa gcgccggcga gttgcagccg gttcnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccccg gcagccactt
     1381 ggttttcagc gcttccatca ccccgataca ggcgccgctc aaccccaaag cattcgatta
     1441 tgcgcgttat caacattata aaaacgttca ctatcagaca tttgtcggtg aagatgcctg
     1501 gcaattgtcg gatcgtccgg cttccttcag tctgtccaaa tacaccgacc gcgcccgccg
     1561 gtactgcctt gctgttttga gaaaacacct gcctacgccc aacgaattcg gagccgcctc
     1621 gccactcatt ctcggctacc gcgacgaaac gcccgatgcc ctccgcgnnn nnnnnnnnnn
     1681 nnnnnnnnnn cacacatgtt ctggcggttt cgggcctgca tgtgggcatc gtacaaatgg
     1741 tcatcagctt tttattgcag ttctggggcc gtaaaagcgg ccggcatcgc atcccaaaaa
     1801 tgctcattac catcctcggc gtgtgggcct tcgcgctcat cacgggcggc gcgggttcgg
     1861 cattgagggc ggccaccatg ttcagtttcc tcacggcagg gcaggccata cggcaaagtt
     1921 ccaacactta caacacgatg gcggcctctc tctttttact gctgtgtatc aatccattat
     1981 tgctgttcga tctgggagtg cagctatcgt atctggcagt actgggcatc gtattttttc
     2041 atccgcgcat ctaccggctt tggtacatcg aaaacaaagc cggcgattat ctgtggcaac
     2101 tgacggccct gtctctggcg gccacgctca ctaccctccc cctgtcgctg ctgtatttcc
     2161 atcagtttcc ggtctatttc tggttgtcgg gattggtggc ggttccgctg tcgggcttca
     2221 ttttgggcgg cggcctcctg ctttttgcgg tgcagggcat ccctgtgcta ggttttgcgg
     2281 tgggcaaggc gctgtacgct ttagtttggc tcatgaatgc ggccatcttt ctcatccagc
     2341 aattgccggg cagccgcatc tccggtattt ggatcagcgt ggcggtggcg gtgctgctgt
     2401 acggcgtcat catagcggtg gcgggcggat tggttaccaa aaaattccgc tggatattgg
     2461 ccgccctgac attgggcgtt ttggcggcag gcatcaatac ctttgaggtc tggcaggcgc
     2521 atcaacgcag ggaaatcgta ctgtatcaca cccgaaggca aacgattatt gattgtttcg
     2581 acggaaggca ggtcttcagc ctttcggcca atcctgtgac ggtggaggaa gaacggtttg
     2641 cgctccagaa ttaccgctgg tatgctcgca gccgaatggc gggcgcctgg ttactgtctg
     2701 atgcgccacc cactgccgaa cgctggtggt atcgcaaggg cttgctgcgc ctgggcgatg
     2761 tccgactggc ggtggtttcg gaaccccaag ctcccttatc tgaaaaaatc ccaaccgatt
     2821 acctcctcct tcggggccgg ccgtacgcat cgctggaaga actggccgag gcctgggata
     2881 tccgatcccg aaccatcctg ttggacggat cgctgtcgcc gcggcaggcg gcccgtttga
     2941 tggaggaagg gcgcgaattg ggctatcaat gttatgatgt ttccaatcgg ggcgcatgga
     3001 tactaaacct gcataaaaca caataaatca aggttgtatg aaacccttaa aagacttttt
     3061 tcactacacc cgctcagagc gaaacggcgc tttgatattg ctgttgcttt gcatcatatt
     3121 gatggtcgca ccgcgtttgt tcgtgttgtt tccttcttcc gaacgcattg attttgagga
     3181 agaaatgcgg ttagccgaac aattggctca gtcttcccgg gaggaggatg gaactccggg
     3241 tacgccggct cagcttttcg agtttgatcc caatacagcc actcaggagg aactgcaacg
     3301 attgggcttg cgcgaacgca ccgtgaaatc tattgtgaac taccgcagca aaggcgggca
     3361 gtttcggaaa aaggaggatt tgaaaaaaat ttataccctg gcggaggagg actatttccg
     3421 gttggaacct tacatccgca ttgcctcgaa cgactcgtat tccaactata attcctacga
     3481 taaaaaagag ccgaaaacct acacgcttgt tgctttcgat cccaacacag ccactcagga
     3541 agagttgtta aatctgggtt tgcccaaagg cgtggtgacg gtgatgctcc gtttccggga
     3601 aaagggcggc aagtaccggc gcaaggagga tttgaaaaaa atatacatcc tcgacgaagc
     3661 cgattacctg cgcatcgagc cgtacatcag cattgccggc gatggcgctt ctatccccac
     3721 cattgctttg aatattcccc aatcgtatga aaatgagaga aatataagca tagacatcaa
     3781 taaagcctcc gccgaagagt ggcaacaatt gcgcggtgtc gggcctgcat tttccggcaa
     3841 gattacccgt ttccgggaag cgttgggcgg ttttacttcc atagaacagg tagctgagac
     3901 gcgtggattg cccgacagca cttttcagaa gatcagatca cagctccgac cctcgccggt
     3961 tttcaggctc attgatgtca atgccgcccc ggcggatgtc ttatccaaac atccttatgt
     4021 tgatcgccgt acggcggagg ccattgtgtc gtatcgccaa cagcatggcg cgtttcgcag
     4081 tgccgacgat ttgcggaaag tgtatgcttt gccggaaggc tgggtggaga agatgaagcc
     4141 gtatttggac tttggaaagt aaaatcgcat acctttgcaa aaaaaattta accatgagaa
     4201 ttgcgattac tttactggcc ttcgcggcca tgttttcaat ggtagcctgc aagcaggatg
     4261 ccaaaaccgg cgcaggcacc tctcccgatc ctgccgcaac agccgtaacc ggagtagtac
     4321 atcactacat ttgcccgaac aactgcgcgg gcagcggcgg tgaagcagcg ggcacatgcc
     4381 ctacatgcgg tacggaatac ctccacaacg atgcattcca caaccagccc ggccaacagc
     4441 ctgctcctgc acccactccg caagtgcaaa cctcgccctc agtggcaacg ccgagcatca
     4501 cgcctccccc atccaccgat ccgccgcaaa atgcagcggg cgtatggcac tacacttgcg
     4561 ccaaaggctg tgcgggcggt agcggagcag ttggcaaatg cgctacctgc ggcggcgacc
     4621 tggcacacaa tgctgcctac catcagtaag gaacgggttc ctaatctgta aaaaat
//
LOCUS       DAORLF010000383         4671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10060_length_4671_cov_2.074740, whole genome shotgun sequence.
ACCESSION   DAORLF010000383 DAORLF010000000
VERSION     DAORLF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_4671_cov_2.074740"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1363
                     /locus_tag="PK198_22025"
     CDS             92..1363
                     /locus_tag="PK198_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF41494.1"
                     /translation="MNTELFIARKVAGAGRKSFSRLIIRIAVVAVALSVAVMIVSSAL
                     ISGFKKEISSKVFGFWGHIHISNTDIGTGFLETRPVSKDQSFYPHLDTLRRIDYMDMG
                     FQRSTKGGVRHIQTYALKPGIIKSKSEIEGIIIKGIGADFDWSFVQRYLIEGEPLDLA
                     DTMPSSDIIISKQTADRLRVSIGDRFIIHFVEQGEQLKRRFTVKGIYKTGLEEYDKVF
                     ALADLRQVQQLLGWSEDQVGGFEVFVDNLDDLNAVADYVYYNQLSNELYAETIREKLP
                     EIFEWLELQDINEVVILAMMIVVALINMITALLILILERTNMIGMLKALGAADRSIRR
                     IFLYYAAYIVGLGLFWGNVIGLGLCFLQKQFRFIRLDEENYYLSEAPVDVQWLSVLAL
                     NAGTLFVILLFLILPSFLVSRISPVKAIRFQ"
     gene            complement(1436..>4320)
                     /locus_tag="PK198_22030"
     CDS             complement(1436..>4320)
                     /locus_tag="PK198_22030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF41495.1"
                     /translation="ETGQPDGAWQQYLIAWQLALRHCGPSHPATADALNGMGDCHYRR
                     SELAQAGQYFRQVLKIRTQFFGMQHEKTASALNNIGNCLASEARYAEAIEMHRAAWHI
                     RQDVLPPRHPEIAVSLSNLGNCAYLAGRPDSAWHYYQEALLIRRERWGEQHPKTALLH
                     NSIGNCLMAMHRPDEALEHFQTALQNTIYALGTQHPTVATIRENLGDLYFDRGDFMVA
                     LDHFRRAYDIHSQVFGEPSVPAMTLWHKIGRCRQYQGEYGLALEHHLAALPPLEASLG
                     TLHPTLGALHNSIGNCYAALHNYPAALRHYGVAQSVFYAQMPAASSDLATTLNNTGAA
                     LLSSGRAADALTFFEKTKPYLAADDHAAQATFFQNKAAASIRLQRYDLATKWLKDAAP
                     HIAGLPVGEQNARRLRQGELYTRIALQRNEAADLRSAADSLYAALLQAGLYRTALDDA
                     RARQVAYARMLPTIRVAMQAFFQLYQQHDNQGFLLRAFQLAEADKSLQWSEISQREWL
                     GESAPLPDSLRQRLRRIHADIHRLEKERLLSTDPAQQHRADLQLAELREQWRHWTRNN
                     EGAAHLGLGDPDGSAATDVAELQKRLLRPQQALIKYFDADSLWLVFVITPEQFKGLAF
                     RKSADFQQKTTAFRQSVYEYIGAPMLVADSLAKRYALLAADLYRELLSPLDASPAAAQ
                     KEWVLMPDGAACYLPFEALLSKMPEDAGLFKKHAYLLKKHTISYAYSAAHWAMLEAAP
                     VEKRLRNMAAFAPDFKAHPSGLPPLRYGAEEARRATALLGGRLFEGRRARSDVFLEEA
                     GRYRILLQATHGRAGRSIGDMSYLAFHHEPGDTAAPVVYSRDLYARHIPAELVVLSAC
                     ETSVGEYRAGEGVVSIAKGFFHAGARSVVATLWSVDDARHADLMLKFLKNISTGERKD
                     AALQKAKLDYISQHPHDEAHPAYWAAATAWGNVRPVTKRSWWRLGK"
     assembly_gap    4321..4450
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          972 a         1254 c         1266 g         1049 t
ORIGIN      
        1 cgaaatcggg aatccgaaat cgggaatccg aaatcgggaa tcgggaatcc gaaatccgaa
       61 atcgggaatc cgaaatcttg aatccaaaat cttgaacaca gaactcttca tagcgcgcaa
      121 agtagccgga gccggtcgca agtccttctc ccggctcatc atacgcattg ccgtagtggc
      181 cgtagcgttg agcgttgccg tgatgatcgt ttcttccgcc ttgatttccg gttttaaaaa
      241 agagatcagc agcaaggttt ttggtttttg gggccatatc catatttcca acacagacat
      301 cggtaccggc ttcctcgaaa cccggcccgt ctccaaagat cagtcgtttt atccgcattt
      361 ggacaccctg cgccggattg attacatgga tatgggcttt cagcgcagca ccaaaggcgg
      421 cgtccgacac atacaaacct atgccctcaa gcccggcatc atcaagtcga aaagcgagat
      481 tgagggcatc atcatcaaag gcatcggcgc cgatttcgac tggagctttg tgcagcgcta
      541 tctcattgag ggcgagccgc ttgacctcgc cgatacgatg ccttcttccg acatcattat
      601 ctccaaacaa accgccgacc ggctccgcgt aagcatcggc gaccgattca tcattcactt
      661 cgtggagcag ggcgagcagc tcaaacgccg ctttactgtc aaagggattt acaaaaccgg
      721 cctcgaagaa tacgacaagg ttttcgcgct ggccgacctc cgtcaggtgc agcaactctt
      781 gggctggagc gaagatcagg tgggcggttt tgaggtgttt gtggacaacc tcgacgacct
      841 caatgccgtc gccgattacg tttattacaa ccaactctcc aacgaactct acgccgaaac
      901 catccgggaa aaactccctg aaatttttga atggcttgaa ttacaggata ttaacgaggt
      961 cgtcatcctt gccatgatga tcgtggtggc cctcatcaat atgatcaccg ccctgctcat
     1021 cctcatcctg gaacgcacca acatgatcgg tatgctcaag gccctcggcg ccgccgaccg
     1081 cagcattcgc cgcatttttc tgtactacgc cgcttacatc gtggggctgg ggctgttttg
     1141 gggcaatgtc attgggttgg gactgtgttt tttgcaaaaa caattccggt tcattcgttt
     1201 ggatgaagag aattactacc tctccgaagc cccggtggac gtgcaatggc tctctgtgct
     1261 ggcgctcaac gcaggtacgc tgttcgtcat ccttcttttt ctgattttgc cttcgttttt
     1321 ggtttcccgc atcagtccgg tgaaggccat ccgattccag taataatacc gccggttccg
     1381 acgattgctg tgtccttcaa ttttgtgcca ttggataaac ataatgattc tgtttttact
     1441 ttcccaaccg ccaccagctt ctttttgtta caggtcttac attgccccag gccgtagctg
     1501 ccgcccaata cgccggatgt gcctcatcgt gagggtgttg tgaaatgtaa tccaatttgg
     1561 ctttttgtaa tgcagcgtct tttcgctcgc ccgtactgat gtttttcaaa aacttcagca
     1621 tcagatcggc atggcgggcg tcgtccacag accacaaagt agccaccaca ctgcgcgccc
     1681 cggcatgaaa aaagcctttg gcaatgctca ccactccctc gcctgcgcga tattcgccca
     1741 ctgacgtttc gcaggcgctc agcaccacca attctgccgg aatatggcgg gcatacaaat
     1801 cgcgggaata caccaccggc gccgcggtat cgcccggctc gtgatgaaac gcgaggtacg
     1861 acatatcgcc gatgctgcgc ccggcccggc catgcgtggc ctgcaacaat atccggtagc
     1921 gcccggcctc ctccaaaaat acatccgaac gggcacggcg gccctcaaaa agccgtccgc
     1981 ccagcaatgc cgttgcccgg cgtgcctcct cggctccata gcgcaaggga ggcaaccctg
     2041 atggatgtgc tttaaaatca ggggcaaaag cggccatgtt gcgcagtctt ttctctaccg
     2101 gcgcggcctc cagcatggcc cagtgcgccg ccgaataggc gtaggaaatg gtgtgctttt
     2161 tcagcaaata agcatgcttt ttgaacagac cggcatcctc cggcatcttg ctcagcaatg
     2221 cctcaaatgg caggtagcag gcggcgccgt cgggcatcag cacccattct ttttgggcgg
     2281 cggccggcga ggcatccaat ggcgacaaca gttcccggta gaggtcggca gccaagaggg
     2341 cataacgttt ggccaagctg tcggccacga gcatgggcgc gccgatgtat tcgtacacgc
     2401 tttgtctgaa tgccgttgtt ttttgttgaa aatcagcact tttgcgaaac gccagaccct
     2461 tgaattgttc cggggtgatg acaaaaacaa gccacagact gtcggcgtca aagtacttga
     2521 tgagggcctg ttgaggccgg agcagccgct tttgcagctc ggctacatcc gtcgcagcac
     2581 tgccgtcggg atcgcccagc cccagatggg cagccccttc attgttacgg gtccaatgcc
     2641 gccattgctc tctgagttcg gccaattgga ggtctgcccg gtgttgctga gccgggtcgg
     2701 tgctcaggag ccgttctttt tccaaacgat gtatatctgc atgaatccgg cgcaagcgtt
     2761 gccgcaaaga atcgggcagc ggcgcacttt cgcccagcca ctcccgttgc gaaatttccg
     2821 accattgcag gcttttgtcg gcttcggcca actggaaagc acggagcaaa aagccttgat
     2881 tatcatgctg ttgatacagt tgaaaaaacg cctgcatcgc cactcgaatg gtgggcaaca
     2941 tgcgggcgta agccacctgc ctggcccgcg catcgtctaa ggcagttcgg tacagacccg
     3001 cctgaagcaa ggctgcatag agtgaatcgg cggcgcttct caagtcggca gcctcgttgc
     3061 gttgcagcgc aattcgggta taaagctcgc cctgacggag tctccgcgca ttttgctcgc
     3121 cgaccggcag cccggcgatg tgcggcgcag catccttgag ccattttgtg gccagatcgt
     3181 agcgttgcag gcgaatggag gcggcggctt tgttttgaaa aaaagtagcc tgcgcagcgt
     3241 gatcgtcggc agcgaggtag ggcttggttt tttcaaaaaa cgtcagcgcg tcggcggccc
     3301 ggcctgaact caggagcgcg gcgccggtat tgttgagcgt agtcgccaga tcggatgaag
     3361 cggcgggcat ttgtgcataa aaaacggact gcgccacgcc atagtgccga agcgcggcgg
     3421 ggtagttgtg cagggcggca tagcaattgc cgatgctgtt gtgcagcgcg ccgagggtcg
     3481 ggtgcagcgt gcccaatgac gcttcgaggg gcggcagcgc agcgaggtga tgctccaatg
     3541 ccaggccgta ttcgccttga tactgccggc agcggcctat cttgtgccac agggtcatgg
     3601 cgggcacgga aggttctcca aatacttgag aatgaatatc gtaagccctg cgaaaatgat
     3661 cgagcgccac cataaaatcg cctctgtcga agtacaaatc ccccaggttt tcgcgtatcg
     3721 tagccacggt ggggtgttgt gttcccaaag catagatggt gttttggagc gccgtttgaa
     3781 aatgttccaa agcttcatcg gggcggtgca tggccatgag gcaattgccg atgctgttgt
     3841 gcaacagggc ggttttggga tgttgttcgc cccatcgttc ccggcgaatg agcaaggctt
     3901 cctgatagta gtgccaggcc gaatcggggc ggccggccag atacgcacag ttgcccaaat
     3961 tgctgagact gacggcaatt tcaggatgcc ggggcggtaa tacgtcttgg cgaatgtgcc
     4021 aggcagcacg gtgcatttcg atggcttcgg catagcgggc ttcggaggcc aggcaattgc
     4081 ctatgttgtt gagtgccgag gctgtttttt cgtgttgcat tccgaaaaac tgggtgcgaa
     4141 tcttcaaaac ctgccggaaa tattgcccgg cttgcgccaa ttcactcctg cgatagtggc
     4201 agtcgcccat gccgttgagc gcatcggcgg tagcggggtg ggaggggcca caatgcctga
     4261 gcgccaattg ccatgcgatc agatactgct gccaggcgcc gtcgggttgg cctgtttcca
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn gccaataagg tacgcgattg ctctatccaa tacgcgggtt gttggcgtcc
     4501 gaagtcaaac ggggcggctt gcatcgaatg agcaagggcc gcacaccaaa cgattatttg
     4561 aacgaagcga ttcattgtgc ttccggtcag cgttttttcg ttactattca gcgggcctcc
     4621 aaaagctacg agctatgagt cacgagctat gagtttgttt attcgatgaa a
//
LOCUS       DAORLF010000384         4671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10061_length_4671_cov_1.965988, whole genome shotgun sequence.
ACCESSION   DAORLF010000384 DAORLF010000000
VERSION     DAORLF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10061_length_4671_cov_1.965988"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    294..346
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            378..641
                     /locus_tag="PK198_22035"
     CDS             378..641
                     /locus_tag="PK198_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41496.1"
                     /translation="MRRFPFFLAPLLGIVALFFLIKVLFVALVGLAVFGLAFATVRAI
                     AARRFHHAYSMTASYQRPTPLDGMYREQGFAAAASQQRIVEII"
     gene            701..1132
                     /locus_tag="PK198_22040"
     CDS             701..1132
                     /locus_tag="PK198_22040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF41497.1"
                     /translation="MCYNHHHGMHGGCGYASKGMGHHGGHWAQRYAGQGFRPPVNIEE
                     LEDRYEIRLAAPGRNSADFQVKVQAGILTISGGRQHNEDASAPRWGRQEYRMESFERQ
                     FQLNDKIDAEGITASYTDGILVVTLPKHPHAHTPLKDIFVS"
     assembly_gap    1424..1544
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1545..>1776)
                     /locus_tag="PK198_22045"
     CDS             complement(<1545..>1776)
                     /locus_tag="PK198_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF41498.1"
                     /translation="HVVLLDELDKAPRDLPNDVLAAIEDLEFQVPEIGKSFRTTAENR
                     PIIVLTSNSEKNLPDPFLRRVVYYHIPFPDAEA"
     assembly_gap    1777..1930
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1931..2186)
                     /locus_tag="PK198_22050"
     CDS             complement(<1931..2186)
                     /locus_tag="PK198_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF41499.1"
                     /translation="MSFFAYKAEGQGQLPPFEPNTQLNNPALYRPSQQLTDAVNVALS
                     LGQPLLLTGEPGTGKTQLAYHIAWFFQLGKPLVVNIQTTAT"
     gene            complement(2396..3490)
                     /locus_tag="PK198_22055"
     CDS             complement(2396..3490)
                     /locus_tag="PK198_22055"
                     /inference="COORDINATES: protein motif:HMM:NF040412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41500.1"
                     /translation="MSSIAEGIKNQLELTIRRQNELREAYAIAFDANQRFAYETQIGQ
                     LDKEIAGLRLQLKDAYGLEMLEESSILEGLVRQLTIDQDVGEIHLVNCDREPPARAFW
                     KAFDEFTGRNNRFQFYFIVACPSQQPDSFAERMVYELIIEELEEQVGAMHYVRRPDSR
                     RMKIEDLPLGRNLANSQREFKKYFSRRFGFGDSETPFEEYLQTGLPKLQYEYVATALD
                     LNAGRWDAALMKEYLAWIMDMFSGTPANVPHFVFFFVIFLRNVHVQPLQGAEKAVFED
                     IGGIVGENLHRATLISELVPVPSALLEDWIRDLGEQNQSRIEDVVKAIVAELSPEKQA
                     KFQQDKLLDMTDIQRFQALIYKIAHAGKGG"
     gene            complement(3556..>4671)
                     /locus_tag="PK198_22060"
     CDS             complement(3556..>4671)
                     /locus_tag="PK198_22060"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRF41501.1"
                     /translation="IVAADTLTAIPFARLRLESRPLRFALLKIGSIAVNIAFIFFFLE
                     WLPILAEQGQGWAQNIWRSDNKVAYVFIANLLASGLVLLYFLPQYVRALAGGFDKKLW
                     RQMLVYSLPLVPAGVAAIINQLVGVPMIKSLASNDLKYNLALAGIFAAATKFAVMMNL
                     FTQAYNYAAEPFFFRHSGSSSDKKVYGQAALAFTLVGCVALLGIVFYMDIIQHLLGKN
                     YREALGVVPILLLANLFLGLYYNFSIWFKLADRTQFGSYIALIGMGITLAINFMFIPR
                     PEMSYYAPAWASLACYGFMALAAWYTGQKHYPIPYPVARMGLYMGLAVALLAAGMGVE
                     YMVGERFWSALAIKTLLLLAFAGFTVRRELGYFRKAG"
BASE COUNT         1028 a         1141 c         1182 g          992 t
ORIGIN      
        1 gaaaaatcat ctaatttgga acaggaaacg aacaaaagga gcgctgctgc tgccgtgaga
       61 agaaagagtc ggaaaaaagt atgcatgcca atcgtttttg ggctgaacaa gaaaatgttg
      121 cgtcgttgag atagaggaga cagtcgctgt aatgaacgcc tcaaaagtcg aaacaggtgc
      181 aaatctgtgc atttttttgg aaaaatcctg catttacggg ccttggcaaa cacagcctca
      241 tttttttcaa aatctttaaa gtaaaacccc ggctcgttcg tcttcccctt tgtnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaag catttattca
      361 ctttaaatca ccttcacatg aggcgtttcc ctttttttct ggcccctttg ctgggcatag
      421 tggccttgtt cttccttatt aaagtgttgt tcgtggcact tgtgggctta gctgttttcg
      481 gactggcatt cgcaactgtg cgggccatag ctgcacggcg gtttcaccat gcgtactcca
      541 tgacggcatc ttatcagcgg cccacgccct tagacggcat gtaccgtgag cagggcttcg
      601 cggctgccgc atcacagcag cgcatcgtcg aaatcatctg ataatttttt tcaaaatggg
      661 cgcagcggac ttaccctgcc ccgccaaaac caattgaatc atgtgttaca atcatcatca
      721 tggaatgcac ggaggctgcg gatatgcctc caaaggaatg ggacatcatg gcggccattg
      781 ggcgcaacgc tatgcaggcc aagggttccg gccacccgtc aatattgaag agttggaaga
      841 ccgctacgaa atacggcttg ccgcaccggg gcgcaacagt gccgattttc aagtcaaagt
      901 acaggccggc atattgacca tctccggcgg acgccagcac aacgaagacg cgtcggctcc
      961 gcgttggggt cggcaggagt accgcatgga gtcgttcgag cggcaatttc aactcaacga
     1021 taaaattgac gccgagggca tcacggcaag ctataccgat ggcattctgg tagtgacgct
     1081 gcccaagcac ccacatgcgc acacgccgtt gaaagacatt tttgtttcgt aaagggaagt
     1141 tttacggcgt tgacggggag gctcgcttcc tccataaagc gggccgcaac cggtcacggg
     1201 aatattcagg ggaggcgctc cggcttgggg cgtctctctt tttttgtatt ttacccttcc
     1261 cagtccttca gtgcttcgag gtcttcgcgg gttttggcca acacagaata actcgtttgc
     1321 aaggtctgcc ggtcctgggc agaaagtcgt ttgccgggag cgagcagcgc agggtcgaat
     1381 tgcagtttgc gcagcagcat ggcccaataa atcagttcgg cggnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaagcct ccgcatcggg
     1561 gaagggaatg tggtaataca ccacccggcg caggaagggg tcgggcaggt ttttttcaga
     1621 attggacgtg agtacaatga tggggcggtt ttcggcggtg gtgcggaaag atttgccgat
     1681 ttcgggcacc tgaaactcca ggtcctcaat ggcggccagc acgtcgttgg gcaggtcgcg
     1741 gggggctttg tccaactcgt cgagcagcac cacatgnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ccgttgccgt cgtctggatg ttgacgacca aaggcttacc caattgaaaa
     1981 aaccatgcga tgtggtaggc caattgggtt ttgccggtgc cgggttcgcc ggtgagcaga
     2041 agcggctgcc ccagcgacag cgccacgttg acggcgtcgg tgagctgctg cgagggccgg
     2101 tacagtgccg ggttgttgag ttgtgtgttc ggctcgaagg gtggcaattg accctggcct
     2161 tccgctttgt aggcaaagaa gctcatgttg gctgttttta tcgtatttat tttgcatttc
     2221 tcatgggctg ttagcttata gctattagcc gccagcttga ttttcgaaga aaggagaaaa
     2281 attcattttc aaataaattt ccccatgtgg aaaatcttct tccttaaaaa aaacaaccac
     2341 gccacggcgt ggcagcagct tgtggctttg aaaaagatgc tacatttttt tttacctatc
     2401 ccccctttcc cgcgtgggca attttataaa tcagcgcctg aaaacgctgt atgtccgtca
     2461 tgtccaacag cttatcctgt tggaatttgg cctgcttttc gggcgacaac tccgccacga
     2521 tggccttcac cacgtcctca atccgacttt ggttttgctc ccctaaatcc cgaatccagt
     2581 cttcgagcag ggcagagggc accggcacca actccgaaat cagggtagcg cggtgcaggt
     2641 tttcacccac gatgccgccg atgtcttcaa aaaccgcttt ttcggcgcct tgcaagggtt
     2701 gtacatgcac gttgcgcagg aagataacga aaaaaaagac gaaatgaggc acattggccg
     2761 gcgtaccgga aaacatgtcc atgatccagg cgaggtactc cttcatcagg gcggcatccc
     2821 agcggccggc gttgaggtcc agcgcggtgg ccacatactc gtactggagc ttgggcaggc
     2881 cggtttgcag gtactcctcg aagggcgttt cgctgtcgcc gaagccgaac ctgcggctga
     2941 agtacttttt aaattcccgc tgcgagttgg ctaagttgcg ccccaacggc aggtcttcta
     3001 ttttcatgcg gcggctgtcg gggcggcgca catagtgcat ggcgcctacc tgctcttcca
     3061 attcctcgat gatgagttca tacaccatgc gctcggcaaa gctgtcgggt tgttgtgaag
     3121 gacaggccac gatgaaataa aactgaaaac ggttgttgcg cccggtaaac tcgtcgaaag
     3181 ccttccagaa agcgcgggcc ggcggttcgc ggtcgcagtt caccaagtgt atttcaccca
     3241 catcctgatc tatggtcagt tggcgcacca aaccttccag aatagaggac tcttcgagca
     3301 tttccagccc gtaggcatcc ttgagctgga ggcgcagccc ggcaatctcc ttgtccaact
     3361 gcccgatctg ggtttcgtaa gcaaagcgct ggttggcatc aaaagcaatg gcgtaggctt
     3421 cccgcaattc gttctgacgg cgaatggtga gttccaattg gtttttgatt ccttcggcaa
     3481 tgctcgacat aacaacactc tgatgaaacg aagggtaaag atagaagttt tttgcaactg
     3541 tggccgcgcc tcctctcacc ctgccttccg aaaatacccc agctcccggc gcacggtaaa
     3601 tccggcaaat gccagcaaca gcagggtttt tattgccaaa gccgaccaaa accgctcgcc
     3661 gaccatatac tctaccccca tgccggcggc caacaaagcc actgccaggc ccatgtacaa
     3721 gcccatgcgc gccacggggt aaggaatcgg gtaatgcttt tgcccggtgt accaggccgc
     3781 cagcgccatg aagccgtagc aggccagcga ggcccaggcc ggcgcgtagt agctcatctc
     3841 cgggcgggga atgaacatga agttgatggc taaggtgatg cccatgccga tgagcgcgat
     3901 gtagctgccg aattgcgtgc ggtcggccag tttgaaccag atggaaaaat tgtaatacag
     3961 ccccagaaac agattggcca acaacaaaat gggcactacg ccgagggctt cgcggtagtt
     4021 tttgcccagc aggtgctgta tgatgtccat ataaaacaca atgccgagca acgccacgca
     4081 gcccactaag gtgaaggcca gagcggcctg tccatatact tttttatcac tggaactgcc
     4141 ggaatgccgg aagaaaaaag gttcggcggc gtagttgtag gcttgggtga agaggttcat
     4201 catcacggca aatttagtag ctgccgcaaa aatgcccgcc agcgccagat tgtattttaa
     4261 atcattggaa gcgagggatt taatcatcgg cacccccacc aattggttga tgatggccgc
     4321 cactccggca ggcactaagg gcaggctgta aacgagcatc tgtcgccata attttttatc
     4381 aaaaccgccg gcaagcgccc gcacatattg cggcaggaag tagagcagca ccaagccgct
     4441 ggccagcaaa ttggcgatga acacataagc caccttattg tcggagcgcc atatattttg
     4501 cgcccaaccc tgaccttgtt ctgcgaggat ggggagccat tctaaaaaaa agaaaatgaa
     4561 ggcgatattg acggcgatgc tccctatctt caacaaagca aaccgcagcg gacggctctc
     4621 caacctcaga cgggcaaagg gaatggccgt gagcgtatcg gctgccacga t
//
LOCUS       DAORLF010000385         4669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10066_length_4669_cov_2.245124, whole genome shotgun sequence.
ACCESSION   DAORLF010000385 DAORLF010000000
VERSION     DAORLF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_4669_cov_2.245124"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK198_22065"
     CDS             <1..582
                     /locus_tag="PK198_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41502.1"
                     /translation="LRLRHTGRDDHFSTWSELLQQSETLKNKLFFAVKRIQEERSEAS
                     RLYLLVYDLEQDLIQSTDEIVTACRNHVENRLSPPEKAQTKSLLTALSKLDDYFKLLS
                     QRMKDRNFANIDDITALRQGALNHLEEMLSIQIAGIKSSKYGFRNTSLMFTLLLETKD
                     LVQISYRFAELYHSLHHNGYLGDYTFLNEPSQT"
     gene            648..1445
                     /locus_tag="PK198_22070"
     CDS             648..1445
                     /locus_tag="PK198_22070"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRF41503.1"
                     /translation="MQHHFTERLYLSHRESKNLPAPSLVCDWLDGILKLLFPELADRR
                     YRHLREFQQHFQDVKLELFTILETLQDALPAPPDQIEEAFLERLPVVHELLLKDADAI
                     LHGDPAAVSATEVIRTYPGFYAIAVYRLAHEFYQLDVPLVPRILTEHAHSRTGIDIHP
                     GASIGERFCIDHGTGVVIGETVVIGDEVKIYQGVTLGALSVRKELAQTKRHPTLQDRV
                     VVYSGATILGGQTVIGHDSIIGGNVWLTKSVPPFSKVYYDHTETGRE"
     gene            1536..1994
                     /locus_tag="PK198_22075"
     CDS             1536..1994
                     /locus_tag="PK198_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41504.1"
                     /translation="MKKNLVFAILVLSLGAAFSAQAQSPENLWKTLGKITFKKQYDEL
                     LGFKVDVPVFSQEILGMEGKIVTVKGYIIPVEGYKSHKEFVFSAYPYNMCFFCGGAGP
                     ETVMEVYAKEAIKYTAEPVTIKGRLELNSTDVNRLIYALNDVVLLTGSSK"
     gene            complement(2004..>3064)
                     /locus_tag="PK198_22080"
     CDS             complement(2004..>3064)
                     /locus_tag="PK198_22080"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRF41505.1"
                     /translation="YTYAIVHCNADWTPSTLAEMEYVDGFAEDRIIDWSFSAKTLKPY
                     TQYTVRLPHNDMRITKSGNYLLKVYEDERDKTLAITRRFMVVEPLTRITARVMNPAQV
                     SKIKTHQEIDFTVDYERINLRAPQQEVWATVLQNGRWDNAITGIPPMFVRKDQLVFDF
                     QDKIVFLGGKEFRFFDFRLLRSRMPGVATIEQVGKSYEVVMEREAVRSGTPYIQYEDI
                     NGQFVIETRDQRNPLSAEYLDVLVSLNAAEPYPNHEVYIIGKLTDWQPKEEFRMVYNP
                     AVNAYVAKLLLKQGFHNYMYALVPRQSKDRRPNTSEMEGDWFETENDYTILIYYRPLG
                     ERYDRIIGVLTHNSNAPR"
     assembly_gap    3065..3074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3075..3286)
                     /locus_tag="PK198_22085"
     CDS             complement(<3075..3286)
                     /locus_tag="PK198_22085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41506.1"
                     /translation="MYRLSLLFALLSFSLLLPAQDEGLAYEDRIYKSNIRSVLFHVNG
                     LVLTQPIIDLSTGVALQLTFDDMDADT"
     gene            complement(3428..4156)
                     /locus_tag="PK198_22090"
     CDS             complement(3428..4156)
                     /locus_tag="PK198_22090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF41507.1"
                     /translation="MHQKPEIIHQDEWLIVAVKPVGMLTIPDRFDASKPNLQDALAAM
                     FPRVWTVHRLDRETSGAIIFALDEDTHRSLSLQFEHRTVEKTYLALTEGVPTPESGRI
                     EQALAPHPGKPGQMMVVKKGKYALTDYRVQETFRGYALVEAQIHTGRTHQVRVHLTHI
                     GHPLVADPLYGRGEALFLSQIKSKDFRMGKLQEEAPLLHRTALHAWRLVFDHPATGER
                     MTVDCPLPKDLKAAVNQLRKWRKA"
     assembly_gap    4331..4403
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4404..>4669)
                     /locus_tag="PK198_22095"
     CDS             complement(<4404..>4669)
                     /locus_tag="PK198_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRF41508.1"
                     /translation="RLWWLFPLRLVLDGLAGLLFLSQGKWRHILSIIQAHFAVYIRWG
                     RLMHRRAQAQKRIEANRINVPADKTGRYRGSIVWQYYARNVHQF"
BASE COUNT         1050 a         1247 c         1224 g         1065 t
ORIGIN      
        1 ctgcgtctcc ggcacaccgg tcgggacgac catttttcca cctggtctga gctgttgcaa
       61 caaagcgaaa cgctgaaaaa caagcttttc ttcgccgtca aacgcattca ggaagaacgc
      121 tccgaggcca gtcgccttta cttgttggta tatgatttgg agcaagacct tattcagtcc
      181 acggacgaaa tagtaactgc ctgccgcaat cacgtggaaa accgcctctc gcctccggaa
      241 aaagcacaaa ccaaatccct tctgacagcg ctctccaagt tggacgacta cttcaagctc
      301 ctttcccagc gcatgaaaga ccgcaatttt gccaacatag acgacatcac agccctgcgc
      361 cagggagcat tgaaccactt ggaagaaatg ctctccatac agatcgcggg tatcaaatcc
      421 tccaaatacg gcttccgcaa tacctctctg atgtttacat tgctgctgga aaccaaagac
      481 ctcgtgcaaa tttcttaccg ttttgcagag ttgtatcaca gcctgcacca caacggctat
      541 ttgggcgatt acacattttt gaatgagccg tctcaaacct gatacctggg gcaaccttga
      601 tgcctgattc ctgttctttg tgaagatttt ctcattgata cagtgtcatg cagcatcatt
      661 tcacagagcg gctctatctg tcgcatcgcg aatccaaaaa cctgcccgct ccttctttgg
      721 tatgcgactg gcttgacggc atactgaaac tactctttcc agaattggcc gaccgccgtt
      781 accgccacct ccgggagttt caacaacatt ttcaggatgt caaattagaa ctatttacca
      841 tcctcgaaac ccttcaggat gccctgccgg ctcctcccga ccaaattgag gaggcatttt
      901 tagagcgcct gccggtggta cacgagttgc tgctcaaaga cgcggatgcc attttgcacg
      961 gcgacccggc cgcagtgagc gccaccgagg tgattcgtac ctatcccggc ttttacgcca
     1021 ttgctgtgta tcgcctggcg cacgagtttt accagctcga cgtgccctta gtgccgcgca
     1081 ttctcaccga acacgcgcac agccgcaccg gcattgacat tcaccccggc gcttccatcg
     1141 gcgagcggtt ctgtatagat cacggcaccg gcgtagtcat cggcgaaaca gtggtcattg
     1201 gcgatgaagt gaaaatttat cagggcgtga cgctgggtgc gctcagtgtg cgcaaggaac
     1261 tggcccaaac caaacgccac cccaccctgc aagaccgcgt ggtggtgtac tcgggcgcca
     1321 ccattctggg cggccagaca gtcatcggcc atgacagcat catcggcggc aatgtgtggc
     1381 tgacaaagag cgtgcccccg ttttccaagg tatattacga tcatacagag acgggccgcg
     1441 agtaagaggg ggaataatag gctctttttt aacacactct tatcatcttt ttcagcacat
     1501 ccgtcgttta tagtggctga aacaaccaac ctgcgatgaa aaagaacctt gtttttgcga
     1561 ttcttgtact gagtttgggc gccgcatttt ccgcacaggc ccaatcgccc gaaaacctct
     1621 ggaaaacact gggcaaaatc accttcaaaa aacaatacga cgaactgctg ggcttcaagg
     1681 tggacgtgcc tgtgttcagc caggaaatcc tcggcatgga gggtaaaatt gtcaccgtca
     1741 aaggctacat catccctgtg gagggctaca aaagccacaa ggagtttgtg ttctcggcct
     1801 atccttataa tatgtgtttc ttttgcggcg gcgccggccc cgaaaccgtg atggaggtgt
     1861 atgccaaaga agccatcaaa tacaccgccg aacccgtcac catcaaaggg cggttggaac
     1921 tgaactctac cgacgtcaac cggctcattt acgccctcaa tgacgtggtg ttgctgacgg
     1981 gttcgagcaa gtaagcgcct gctttatctc ggcgcattgg agttgtgcgt cagcaccccg
     2041 atgatgcggt cgtagcgctc gcccaagggg cggtaataga tgagaatggt gtaatcattt
     2101 tccgtttcga accaatcgcc ttccatttca gaggtgttcg ggcgccggtc tttgctttgc
     2161 ctgggcacca atgcgtacat gtagttgtga aagccctgct tgagcagcaa cttcgccaca
     2221 taagcattga cggcagggtt ataaaccatc cggaactcct ctttgggctg ccagtccgtc
     2281 agttttccga tgatatagac ctcgtgatta gggtagggtt cggcagcatt cagcgaaacc
     2341 aatacatcca gatactcggc agagagcggg ttgcgctgat cgcgggtttc gatcacaaat
     2401 tgaccgttga tgtcctcgta ctgtatataa ggcgtgccgc tgcgcacggc ttcgcgctcc
     2461 atcacgactt cgtaagattt gcccacctgc tcaatggtag ctacgcccgg catccgcgag
     2521 cgcaggaggc gaaaatcgaa aaagcgaaac tccttaccgc caagaaaaac gatcttgtcc
     2581 tgaaagtcaa ataccaactg gtctttccgc acaaacatag gcgggatgcc cgtgatagca
     2641 ttgtcccagc ggccgttttg cagtaccgtt gcccacactt cctgctgcgg tgcgcggaga
     2701 ttgatgcgct cgtagtccac cgtaaaatca atttcctgat gggttttgat tttgctcacc
     2761 tgcgccgggt tcattactct ggccgtgatg cgggtcagcg gttccactac catgaaacgg
     2821 cgggtgatgg ctaaggtttt atcgcgttcg tcttcataca ctttgagcag gtaattgccc
     2881 gatttggtga tgcgcatgtc gttgtgcggc aagcgaacgg tgtactgcgt gtagggcttg
     2941 agggttttgg ccgaaaagct ccagtcaatg atgcggtctt cggcaaagcc gtccacatat
     3001 tccatttcgg ccagggtaga gggcgtccag tctgcgttgc aatgcacaat ggcgtaggtg
     3061 taatnnnnnn nnnngtatcg gcgtccatgt cgtcaaaagt gagttgcagc gctacgccgg
     3121 tactgaggtc aatgatgggc tgggtgagta ccaatccatt gacgtgaaaa aggacgctgc
     3181 gaatattgct tttgtaaatg cgatcttcat acgccaagcc ctcgtcttgt gccgggagca
     3241 gcagcgaaaa ggacaacagc gcgaagagga gagatagacg gtacatggta ataatttttg
     3301 ggtggtttag tgaaggggtg actctgccaa gatcacaatc cgtagtcacc ccttcactat
     3361 gcacagtata agacgcaaaa ctaacgcaga aagcgcgggt tttgggatgc aggcggagag
     3421 atatttccta cgccttccgc cacttcctca gttgattcac cgctgctttg aggtctttgg
     3481 gcagcgggca atccaccgtc atgcgttcgc cggtagccgg atgatcgaac accaaacgcc
     3541 aagcgtgcag ggctgtgcgg tggagcaggg gcgcttcttc ctgcaatttg cccatgcgga
     3601 agtctttgga tttaatttgc gacaaaaaca aggcctcgcc ccgaccatac agcgggtcgg
     3661 ctaccaatgg atggccgata tgcgtcaggt gtacccgcac ctgatgcgtg cggccggtgt
     3721 gtatctgagc ctccactaag gcatagcccc gaaacgtttc ctgcacacgg taatcggtga
     3781 gggcgtattt gccttttttc accaccatca tctggccggg cttgccgggg tgcggcgcca
     3841 atgcctgctc gatgcgcccc gattcggggg tggggacgcc ctcggtcagc gccaggtagg
     3901 ttttttctac ggtgcggtgt tcaaactgta aggagagcga gcggtgcgta tcttcatcaa
     3961 gcgcaaaaat gatggcgccg ctggtttcgc ggtccaagcg atgcaccgtc catacgcggg
     4021 gaaacatggc cgccagcgcg tcctgtagat tgggcttgga ggcgtcaaaa cggtcgggaa
     4081 tggtgagcat gcccacgggt ttgacagcta cgatgagcca ctcgtcttga tggataattt
     4141 ccggcttttg atgcatctgt tatgtattgt gatctgcttc gcagttgatc gcttgcccgc
     4201 gccttgcgcg gtaaacgagg ggaaacccgg tagccgcgcc tgtggcgcgg ggtaggcggt
     4261 gctaagcatg agagtacccg aaaccttaaa cccgaaaccc gccttcgcca aggcttcggc
     4321 ggatgaaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnngaattgg tgtacattgc gtgcataata ctgccacacg
     4441 atgctgcccc ggtagcggcc tgtcttatct gccggcacat tgatgcggtt ggcttctatg
     4501 cgcttctgag cttgcgcgcg acggtgcata aggcggcccc atcgaatgta aacggcaaag
     4561 tgcgcctgaa tgatggaaag aatatgccgc cacttgccct gcgacaaaaa cagcagcccg
     4621 gccagcccgt caagtaccaa tcggagcgga aacaaccacc acaaacgcg
//
LOCUS       DAORLF010000386         4664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10079_length_4664_cov_2.092862, whole genome shotgun sequence.
ACCESSION   DAORLF010000386 DAORLF010000000
VERSION     DAORLF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_4664_cov_2.092862"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..579
                     /locus_tag="PK198_22100"
     CDS             205..579
                     /locus_tag="PK198_22100"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRF41509.1"
                     /translation="MADIKGKAPQESGSKSVPTSQGLNCLVAKGTRIEGQFAAAEDTR
                     IDGEIKGEVKCERKLVTGESSHIEGKIWAAEAVFMGSITGDIVVSGSVQLTSSAVITG
                     TLSAKTLSVDEGAQLNGAFKIA"
     gene            685..1272
                     /locus_tag="PK198_22105"
     CDS             685..1272
                     /locus_tag="PK198_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41510.1"
                     /translation="MRIVINIVLLLATVGLAYLLYSSIQEPILFKKERTRREDAVTAR
                     LVQIRTAQEAYRDITGRYAPNFDTLKEVLSTGKFKIVSVIGDPDDPNFTGKITYDTIF
                     RPALDSIKALGIDLDGIRYIPFSDKKDMFEMQADTLTYQGTLVQVVECKAQRTTFMGK
                     FDASYAKYDKAYDPMSYLKFGNMTQPNLSGNWESK"
     gene            1257..2081
                     /locus_tag="PK198_22110"
     CDS             1257..2081
                     /locus_tag="PK198_22110"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRF41511.1"
                     /translation="MGIKIISEYKDPHFRQGSPAEHKLSVLLRPDGLCFHVTDAAHST
                     SFAWKELAGDTANELLAAAGREPLLSGTAYSSAILATCGPAALAPARLFDPSHAADYW
                     LSLSDLPAGYSVQADAIPVLDAYVVYPLDGQSVQALPGARNIHLATAWLSGIYRHALR
                     ETGPAIYAHTSGKQLMLAAFDGRALRFFNTFTFQTAQDFLYFVLLALDQAQFALEQTL
                     LFVSGKMTSDSEIHLLLQRYFVDMQFLAPAAAAPYPAGLPDDRRHWYFDLACIGSL"
     gene            2101..2682
                     /locus_tag="PK198_22115"
     CDS             2101..2682
                     /locus_tag="PK198_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRF41512.1"
                     /translation="MRIISGKFKGRRFFPPADNWPTRPTTDFAKEGLFNVLNNHFDFE
                     DIKVLDLFGGTGNHSYEFISRGCTDVTYVDKFNGCVEFVRKTAQALQVESCLRIVRAD
                     VFRFIATCREQYDYIFAGPPYPLPNIPEIPDLIFSFDLLKPGGWLVLEHNPNHDFKGH
                     PRFADARHYGKTIFSIFHAASDAGEDATEEEVA"
     assembly_gap    2735..2871
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2872..3142
                     /locus_tag="PK198_22120"
     CDS             <2872..3142
                     /locus_tag="PK198_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41513.1"
                     /translation="KGRSYVFVDNIFAVIELSGEVSGNNISIREFRIVDSKKTKDLEW
                     CIKTYKLTYSKSGNTRQLSGSWTGHTNGTPCIPGKIILTRQISRA"
     assembly_gap    3267..3290
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3291..3880
                     /locus_tag="PK198_22125"
     CDS             <3291..3880
                     /locus_tag="PK198_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF41514.1"
                     /translation="TFVSPMDALAWLQNHPVDLIFSDIQMPDLFGTEFMRLAKSSGAL
                     FVFVTAHPQYAIEGFQLQALDYLLKPVELPRFIETCNRALQQKQTQAGQQQSIFVKDG
                     HDWLRIQIADIEFIRSDTNLLFIHHAGQTTVTRMTIGRLLELLPAGEFVRVHKSYTVA
                     LKAVKKIERHQLTVGGALIPVARSFREEVERLLLG"
     gene            complement(3892..4467)
                     /locus_tag="PK198_22130"
     CDS             complement(3892..4467)
                     /locus_tag="PK198_22130"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF41515.1"
                     /translation="MTATQKKTTYSVAEYLLLERRTGERYEFYNGKIRRMAGGTIAHN
                     RITRNIIIKLGQLIDEKGNMEIVASDQKIYLPKYGWYVYPDAVVVAGVPIESEEEASA
                     IINPILIFEVLSPSTMDYDRNRKFLEYQSLPSFQEYVLVRQDAPEALLAFREEPDLWR
                     SSETAGMDADIVLRSIGASLPMKDVYAKVEF"
BASE COUNT         1081 a         1219 c         1079 g         1124 t
ORIGIN      
        1 acttatctaa tcctttggcg aaaatgatca ctttattttt ttatcgcccc tgagcgcttg
       61 gcaaacgaac gacaactttg cgggttcgta aacatcactt tgatgtgaaa ttttaccgaa
      121 gttctgtttt ttccggcaaa gtttaatatt tttcggcggt tttgtaaaaa tcactttttc
      181 cattcattca ttgcagtcat cattatggca gacataaaag gcaaagcgcc gcaggaatca
      241 ggttctaaaa gcgtcccgac atcgcagggg ctcaactgtt tggtagcgaa aggcacgcgc
      301 attgaggggc aatttgccgc agcagaagac acccgcattg atggcgaaat caaaggcgag
      361 gtaaaatgcg agcgcaaatt ggtaacgggc gagagcagcc acatcgaagg aaaaatatgg
      421 gcggcagagg ctgtttttat gggttccatc accggagaca tcgtggtaag cgggtctgta
      481 caactgactt cttcagcagt gattacaggc actctaagcg ccaaaacgct atcggtagat
      541 gagggcgctc agctcaatgg cgccttcaaa attgcctgaa gcggcgtttt ttccacatga
      601 ttgttttatg tgtggagctt ttgtcataaa attgcaccgg cattcggcta aaaagttcag
      661 tacttgtatc aaaacaaacc gaaaatgaga atcgtcatca acattgttct tcttcttgcc
      721 accgttgggt tggcttatct gctctacagc agcattcagg agcctatttt gttcaaaaaa
      781 gaacgcaccc gccgcgaaga cgccgtcact gcacggctgg tacaaatccg cacggctcag
      841 gaggcttatc gggacatcac cggacgctat gctcccaact ttgacacact caaggaggtg
      901 ctctcaaccg gcaaattcaa aatcgtgagt gtcattggcg accccgacga tcccaacttt
      961 accggaaaaa tcacttacga taccattttc cgccctgctt tggactccat caaagcgctg
     1021 ggaattgact tggacggcat ccgctacatt cctttttccg acaagaaaga catgtttgaa
     1081 atgcaagccg acacccttac ttaccaaggc actttggtac aagtggtgga atgcaaggcc
     1141 caacgcacta cgttcatggg caagtttgat gcctcctatg ctaagtacga taaggcatac
     1201 gacccgatgt cgtacctcaa atttggcaac atgacccaac caaacctctc gggcaattgg
     1261 gaatcaaaat aatttcagag tacaaagacc cgcatttcag gcaagggtct cctgccgaac
     1321 acaagctgtc tgtgcttttg cgcccggacg gcttgtgttt tcatgtgacg gacgccgcgc
     1381 acagcacgtc ttttgcatgg aaagaattgg caggagatac tgctaatgaa ttgctggctg
     1441 cggccggtcg ggaaccgctc ctctccggca cggcttattc gtctgccatt ctggcgacct
     1501 gcggtcctgc tgctttagcg cctgcgcgat tgtttgatcc ctcgcacgct gccgactact
     1561 ggctcagcct cagcgatttg cccgccgggt acagtgttca ggctgatgca attcctgtat
     1621 tagatgccta tgtcgtttat ccgcttgacg ggcagtcggt gcaggctttg ccgggagcgc
     1681 gcaacattca tctggcaact gcctggctca gcggcattta tcgccatgct ttgcgggaaa
     1741 ccggcccggc catatatgct catacatcgg gcaaacaact gatgttggcc gcttttgacg
     1801 gtcgggcatt gcgctttttc aatacgttta ctttccaaac agctcaggat ttcctctatt
     1861 tcgtattgct ggcgctcgat caggcccagt ttgcgttgga acaaacattg ctgttcgttt
     1921 ccggcaaaat gacctccgat tccgaaattc atctcttatt acagcgatat ttcgttgaca
     1981 tgcaattcct tgcgcccgct gctgccgccc cctaccctgc cggcctgccc gacgaccgac
     2041 gccattggta ttttgatttg gcctgcatcg gaagtttgta acctttgctc tacctccatc
     2101 atgcgcatca tttcaggaaa attcaaaggc cggcggttct tcccgcctgc cgacaactgg
     2161 cccacccgcc cgactacaga ctttgccaaa gaaggtctgt tcaatgtact gaacaatcac
     2221 tttgattttg aagacattaa ggtgctcgat ctctttggcg gcacgggcaa tcatagttat
     2281 gaattcatct ccagaggctg caccgacgtc acttatgtgg acaaattcaa cggctgcgtg
     2341 gagtttgtgc gcaaaacagc acaggcgctt caggtagaat cctgcctccg cattgtccgc
     2401 gccgatgtgt ttcgctttat tgccacctgc cgggagcaat acgactacat ttttgccggg
     2461 ccgccttacc ccttgcccaa cattcccgaa atacccgacc tcattttttc gtttgacctg
     2521 ctgaaacccg gcggctggct cgtgctggag cacaatccga accacgattt caaggggcat
     2581 ccacgatttg cggacgcgcg ccactacggg aaaactattt tcagcatttt tcacgcagcc
     2641 tccgatgccg gggaagacgc aacggaagaa gaggtagcgt aaaggctgcg gataaaacca
     2701 attaaaacta tgagattttt gcttccggct attcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaaaggacg
     2881 ctcttatgtt tttgtggata atatcttcgc cgtcattgag ttgagtggcg aagtgagcgg
     2941 caacaacatc agcatccgag agtttcgcat cgttgactcc aaaaaaacca aagatttgga
     3001 gtggtgcatc aaaacctaca aactcactta cagcaagtcg ggcaataccc ggcaactcag
     3061 cggctcctgg acaggccaca ccaacggaac cccttgcatc cccggaaaaa tcattctgac
     3121 aaggcaaata agcagagcct gaaatactca gatcttcagg cacttaacta cttttcaaca
     3181 cgcttcgaca tgaccctcca agccatagcc atagacgacg aagcgcccgc gctggccatc
     3241 atagccgcac acgccgccaa agtgccnnnn nnnnnnnnnn nnnnnnnnnn ccaccttcgt
     3301 cagtccgatg gacgccttgg cctggctgca aaaccatccc gttgacctca ttttttcaga
     3361 catacaaatg cccgacctct tcggcaccga gttcatgcga ttggccaaaa gctccggcgc
     3421 cctgtttgtg ttcgtcaccg cgcatccgca atatgccata gagggttttc agttgcaggc
     3481 attggactac ctgctcaagc cggtggaatt gccccgtttt atagaaacct gcaaccgggc
     3541 gctccaacaa aaacagaccc aagccgggca acaacaaagc atttttgtaa aagacggcca
     3601 cgactggctg cgcatccaaa tagctgatat tgaatttatt cgatcagata ccaacctgct
     3661 ttttattcac catgccggcc agacgactgt gacgcgcatg accatcgggc gactgctgga
     3721 gctgcttccg gcgggcgagt ttgtgcgggt gcataagtca tacaccgtgg cgctcaaggc
     3781 ggtgaaaaaa attgaacggc accaattgac ggtgggcggg gcgcttattc cggtggcgcg
     3841 ctcgtttcgg gaagaggtag agcggttgtt gctggggtga tggctattct ctcaaaactc
     3901 cactttcgca tacacatctt tcatcggcag gctggcgccg atgctgcgca atacgatgtc
     3961 ggcatccata cccgctgttt ccgaactgcg ccacaggtcg ggttcttccc gaaaggccag
     4021 cagggcctcg ggcgcgtctt gccgcaccag cacatattcc tgaaacgaag gcagactttg
     4081 atattccagg aactttcggt tccggtcata atccattgta cttggcgaca gtacctcaaa
     4141 tatcaaaatg ggattgatga tggcgcttgc ttcctcctcg ctttctatcg gcacgcccgc
     4201 cactactacg gcgtcggggt aaacatacca gccgtacttg ggcaggtata tcttttgatc
     4261 ggaagcaaca atctccatgt tccctttttc atctatcaac tgccccagct tgatgatgat
     4321 attcctggta atgcggttgt gggcaatagt tcctccggcc attcgtctga tttttccatt
     4381 atagaattcg taccgctcac ccgtacgtct ttccaggagc aagtattcgg caacggaata
     4441 ggttgttttt ttctgcgtgg cggtcatggc tgccggattt ttgtacaaag ttacagaaat
     4501 ttattcaaag tttccacgct gcattcctct gccggggtgc atggggttca tccagatacc
     4561 gcagcgccat caacgcgaac aagatgaaaa atgccgtgcc gagttgcgtt tccagcgtgt
     4621 gttccaccat gcacgaagag agcatgagca cataaaaagc agtc
//
LOCUS       DAORLF010000387         4663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10083_length_4663_cov_2.099175, whole genome shotgun sequence.
ACCESSION   DAORLF010000387 DAORLF010000000
VERSION     DAORLF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10083_length_4663_cov_2.099175"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..2251)
                     /locus_tag="PK198_22135"
     CDS             complement(308..2251)
                     /locus_tag="PK198_22135"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HRF41516.1"
                     /translation="MIALPIPASVFTFALCCLMALPARISAQVNVLQHAALLIPQEMK
                     DNAYQVVRLEHTSFQLKDPKEGILKVRKIVTLLDQRNTEDELVLLYDKHTKVNSLIVN
                     VYDALGQHVRKISLRDKEVEDLAAIDGFSIYNDSRIKRLKVAHTSFPFTIDYEYEMTL
                     RGLQLAVFPDWDIQGFHTSVEQSRFEMELPANIQLLYKPLNINVVPQESKTPQGKKTM
                     VWEARDLRAIPREPYAPAPASVLPRVIYSSSQFQIDSYAGSMSDWKSFGSFVNSLYQG
                     RDALPANIQAEVQRIVAQSPDDVAKIHALYRFLQRDMRYVSVQTGIGGWQPFDAIYVA
                     ENKYGDCKALSNYMKALLREAGIEAWPVLIKNGPLDYDVTQDFATSHFNHVILYVPGK
                     DIWLECTSSDMPPNYIGADNSDRNVLLITPQGGVLKRTPIQDAEMNREVNSVLVEVRP
                     DGSAALRYNSRATGEPHEVLRYLHLHVSKEEQGKWLLKKNPLPTLQITHFETSAETDA
                     PAAGLQYQAELKQLGARTGKRWFVPVNLLNAFSEVPPAGPEQRRLPVVLTQHSSETDT
                     IRFVLPDNYTLESYPQQTIEIQSDFGHYALRFEVSGNEVTAIRTLRLQPAQAAAERYP
                     EFRNFFRDIAKNDGLKLVLKQKE"
     gene            complement(2348..2470)
                     /locus_tag="PK198_22140"
     CDS             complement(2348..2470)
                     /locus_tag="PK198_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41517.1"
                     /translation="MFRSKVLDKKYDTELTAHGVTDILRLAIAVEGKEVIVEEV"
     gene            complement(2528..2746)
                     /locus_tag="PK198_22145"
     CDS             complement(2528..2746)
                     /locus_tag="PK198_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41518.1"
                     /translation="MEYKVTINPYKEKEFLQLLSAWESLGVVTHYEVLSPDPCNDLLP
                     QHETALRRPRKKVDTAWEVAEPYKDLLD"
     gene            complement(2774..3814)
                     /locus_tag="PK198_22150"
     CDS             complement(2774..3814)
                     /locus_tag="PK198_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF41519.1"
                     /translation="MSEIILTVEGVDPLELYGENNAKLNLLRKAFPDITITSRGSSIK
                     MAGEKKYTQGAKAKLEMMVRTLRETKELSIQAVEDLLNGDNPFEVRLMSNGHDNQTIL
                     HGTEGRQIKAKTRNQKKLIDAAEHNDIVFAVGPAGTGKTYTAVALAVRALKNRLVKKI
                     ILTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDALDDMIPADKLQQFMTNRTIEIAPL
                     AFMRGRTLDNAFIILDEAQNCSTLQIKMFLTRLGPTAKCIVTGDLSQIDLPFHQKSGL
                     KRAIDLLNGLEGISTVYLDADDVVRHRLVKEIIKAYQRSDEELRRKREEQEAAKSMPS
                     PDAQSADHQGDH"
     gene            4019..>4663
                     /locus_tag="PK198_22155"
     CDS             4019..>4663
                     /locus_tag="PK198_22155"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRF41520.1"
                     /translation="MQIVTLTTDLGEQDYALPALKGCLLQKAAPLLLADVTHQVQNFD
                     IVQAAFILSNVWKHYPAGTIHLVWVNNYYAPHRDFLVISHQGHYFIGPDNGVFSLVLA
                     PAPEQAFRLEYEDNDAFSLRELYAFAVAHIAGGQPLEKVGLPAGSILQRITFQPVTGP
                     SHIRGTVIHVDNFENVVVNIRREVFEHTANGRSFSLYFKRNAPILQLSANYCDVT"
BASE COUNT         1052 a         1144 c         1202 g         1265 t
ORIGIN      
        1 ttgtcgtaaa cggtcatttt caaatcctcg tcgggtactt ttccccagga aattttggca
       61 tcttgcgcga aagcgcccat tgcgagcagg aaagccgcca gggccaggat gaagtgtttg
      121 aggtagttgt gcatggttga gtattttaaa attcaaagtt tcgggtttca tccgccgtag
      181 ccttggcgaa ggcgggtttc gggtttcatc cgccgtagcc ttggcgaagg cgggttcaag
      241 gtttcgggtt gctcacatat tcactcctca cacactcacc cctcacccct aaaaaccgca
      301 taccgattta ctccttctgt ttcagcacca atttcaggcc atcgtttttg gctatgtctc
      361 tgaaaaaatt tctgaactcc gggtatcgtt ctgcggcagc ctgtgcgggt tggaggcgaa
      421 gtgtgcgaat ggcagttacc tcattgccgg aaacctcgaa ccgcaaggcg taatggccga
      481 aatcgctctg aatttcaatg gtttgctgag gataactttc caaagtatag ttgtcgggca
      541 gcacaaagcg gatggtatct gtttcgctgc tgtgttgggt cagcacgacg gggaggcggc
      601 gctgttccgg gccggcagga ggcacctcgc tgaaggcatt gagcagattg acgggtacaa
      661 accagcgctt tccggtgcgg gcgcccaatt gtttcaactc tgcctgatat tgtaatccgg
      721 ctgccggagc gtctgtttct gctgaggttt caaaatgagt aatctgcaaa gtgggcaaag
      781 gatttttttt cagcagccat tttccctgct cttctttcga gacgtgcagg tgcaggtagc
      841 gcagcacctc atgcggttct cccgtggccc ggctgttgta ccgcagcgct gcactgccgt
      901 cggggcgcac ctccaccaaa acagaattga cttcccggtt catctcggca tcctgtatgg
      961 gcgtgcgctt caacactccg ccttgtggcg taatgagcag cacattgcga tcagagttat
     1021 cggcgccgat gtagttgggc ggcatgtcgg aggaggtaca ttccaaccag atatccttgc
     1081 cgggtacata caaaatgacg tggttgaaat gcgaggtggc aaagtcttgg gtgacatcat
     1141 aatccaacgg cccgttttta atcaaaaccg gccaagcttc tatacctgcc tcccgaagca
     1201 gcgctttcat ataattgctg agggctttgc agtcgccata tttgttttcg gccacataaa
     1261 ttgcgtcgaa gggctgccag ccgccaatac ctgtttgaac gctgacatag cgcatatcgc
     1321 gttgcaaaaa acgatagagc gcgtgtattt tggccacgtc gtcgggcgat tgcgccacaa
     1381 tgcgctgcac ttcggcttgg atatttgccg gcaaggcgtc gcggccctgg tacaagctat
     1441 tgacaaaaga accaaatgat ttccaatcgc tcatactgcc tgcgtagctg tctatctgaa
     1501 actgcgaact tgaatagatg acccgtggaa gcaccgaggc aggtgcgggc gcgtagggtt
     1561 cgcggggtat ggcccgaagg tcgcgggctt cccaaaccat cgtttttttg ccttgcggcg
     1621 ttttggattc ttgcggaaca acgttgatat tgagcggttt gtacaacaat tgaatattcg
     1681 ccggcagttc catctcaaac cggctttgct ctaccgaggt atgaaaaccc tgaatatccc
     1741 aatcgggaaa aacggccaat tgcaaaccgc gcagggtcat ttcgtactca taatctatgg
     1801 tgaagggaaa agaagtgtgc gccactttga gccgcttgat gcgcgaatcg ttgtaaatag
     1861 agaagccatc tatggcagcc aagtcttcaa cctctttgtc tcgaagactt attttgcgaa
     1921 cgtgctgccc caaagcatcg taaacattga caatcagtga attgactttg gtgtgtttgt
     1981 catacagcag cacaagttcg tcttctgtat tgcgctgatc gagaagcgtg acgatttttc
     2041 gcaccttcag aatgccttct ttggggtctt tgagttggaa ggaggtatgt tccaatctca
     2101 ccacctgata cgcattgtct ttcatttcct gcggaatcaa aagggcggca tgttgcaata
     2161 cattgacctg cgccgaaatt cgggcaggaa gagccataag gcagcataag gcaaatgtga
     2221 aaacggaagc cgggattggt agagctatca tttagaagag tttatttttg tacagagggg
     2281 tgatctcggt tgcacaaaga taaaaccggt tgttcagaaa tacaaaattt taacacactg
     2341 ggagtagcta tacttcttct acgatgacct ctttcccctc caccgcgatg gccaaccgca
     2401 ggatgtccgt cacgccatgg gcagtgagtt ccgtgtcgta ttttttatcc aataccttag
     2461 agcggaacaa tgccctgact actatgctga tacaaccaaa atggggttgt tgttttgaaa
     2521 taaaagatca gtccagcaaa tctttgtacg gctcggctac ttcccaagcg gtatccactt
     2581 ttttgcgcgg ccggcgcagg gccgtttcgt gttgcggcaa caggtcgttg cacgggtcgg
     2641 ggctgagtac ttcgtaatgc gtcaccacgc cgaggctttc ccaggcgctg agcaactgga
     2701 gaaattcctt ttctttgtaa gggttgatgg tcactttgta ttccataaaa gtatgtttaa
     2761 ttaaaaaaat ggatcaatga tcgccctgat gatcggcgct ttgagcatcg ggagaaggca
     2821 tggatttggc ggcctcctgt tcttctcttt ttctgcgaag ctcttcgtcc gaccgctggt
     2881 atgccttgat gatttccttc accaatcggt ggcgcaccac atcgtcggca tctaaataaa
     2941 cggtagaaat gccttccaaa ccgttgagca ggtcaatggc gcgtttgagg cccgattttt
     3001 ggtgaaacgg caggtcaatt tgcgataagt cgccggtgac gatgcacttg gcggtaggcc
     3061 ccaatcgggt gaggaacatc ttgatttgca gggtagaaca gttctgcgct tcatccaaaa
     3121 tgataaaggc attgtcgagc gtgcgcccac gcataaaagc caatggtgca atctcgatgg
     3181 tgcggttggt catgaactgc tgcaatttgt cggcagggat catgtcgtcc aatgcgtcgt
     3241 aaagcgggcg caggtagggg tccacttttt ctttgagatc gccgggcaga aaaccgaggc
     3301 tctcgcccgc ttcgaccgcc gggcgagtaa ggatgatttt tttgaccaac cgatttttga
     3361 gtgcgcgcac ggccaatgct acggcggtat aggttttgcc tgttccggcc ggacctactg
     3421 caaaaacaat gtcgttgtgt tcggcggcgt ctatgagttt tttctggttg cgggttttgg
     3481 cctttatctg ccgcccctcg gtgccgtgca ggatggtttg gttatcgtgg ccattgctca
     3541 tcaggcgcac ctcgaagggg ttgtctccgt tgagcaaatc ttcaaccgcc tgaatagaca
     3601 actctttggt ttctcgcaaa gtacgcacca tcatttcaag tttggctttg gcgccctgcg
     3661 tatatttttt ctcgcccgcc attttgatgc tgctgccgcg ggaggtaatg gtgatgtcag
     3721 ggaaagcctt gcgcagcaaa ttcagttttg cattgttttc gccgtagagt tccaatgggt
     3781 ccacgccttc gactgtcaga ataatctcgc tcaaaagttt tgttgtttaa atgaaaccaa
     3841 gttctttttt ttggaagcag acacacaacc ggaggctgtc aaaaaaagtt catccggctt
     3901 tttaaatctt tcccaccaaa ggtacgccga gcgttccaaa catctcttac gttttgtctg
     3961 aaatatttat ccacattctt ttctaatttt accgcttcat cacagcagtt gccttttcat
     4021 gcaaatagta acactgacaa cagacctcgg agagcaagac tacgccctcc cggcactcaa
     4081 aggttgcctc ctgcaaaaag ccgcgccttt gttgctcgcc gatgtgacgc atcaggttca
     4141 aaacttcgat attgtgcagg cggcctttat actgagcaac gtctggaagc attatccggc
     4201 tggaactatt catttggttt gggtcaacaa ttactatgcc ccacatcgcg attttttagt
     4261 catttcacat caggggcatt atttcatcgg ccctgacaat ggcgtgttct cgctggtgct
     4321 ggcgccggcg ccggaacagg ctttcaggct tgagtacgaa gacaacgacg ccttctcgct
     4381 gcgggaattg tatgcttttg ccgtggcgca catcgccggt gggcagcctt tggaaaaagt
     4441 gggactaccg gccggttcta ttttacaacg cattactttt cagccggtta caggcccgtc
     4501 gcacatccgg ggtaccgtta ttcatgtaga caactttgaa aatgtagtgg taaatatccg
     4561 ccgggaagta ttcgagcata ctgcaaatgg tcgttcgttt tccttgtatt tcaaacgcaa
     4621 tgccccaatt ctgcaacttt ccgccaatta ttgcgatgta act
//
LOCUS       DAORLF010000388         4639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10145_length_4639_cov_1.908377, whole genome shotgun sequence.
ACCESSION   DAORLF010000388 DAORLF010000000
VERSION     DAORLF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10145_length_4639_cov_1.908377"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..587
                     /locus_tag="PK198_22160"
     CDS             105..587
                     /locus_tag="PK198_22160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF41521.1"
                     /translation="MEKTYIICVEDQREVLNTVLKDLEIFEGPFFLEGCESADEAEEL
                     LDELDAQGDYVGLMVCDHVMPGKSGVQLLIDVNKDGRFRHTKKLLLTGLATHQDTIEA
                     INQAAIDRYIEKPWNVEILQKQARILLTRFLFDQGLNYEPFLPVLDKETVLERLRRMG
                     "
     gene            complement(596..>1080)
                     /locus_tag="PK198_22165"
     CDS             complement(596..>1080)
                     /locus_tag="PK198_22165"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRF41522.1"
                     /translation="PGFSDQDVWNALRMVVPKVLSYILSFMVLAIMWLNHHVLFERIP
                     HSTPRLVWYNMFLLFAMSLIPMPTHFLAAHPFLPQAVMFYGLVMFLNAFGFLLLRRYA
                     AIEGQLIPYSAVVHRSNVVSTLLYLSSVLLAFVSVYLSFIIFIGIPVWYFLPERLSRR
                     "
     assembly_gap    1081..1107
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1189..>1821)
                     /locus_tag="PK198_22170"
     CDS             complement(1189..>1821)
                     /locus_tag="PK198_22170"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRF41523.1"
                     /translation="PQYVKERSEAFKKEMVYLLIAWGHALRLHLRRQDDWEALRPLLS
                     EAAFRSVAASKNQPNALQLLTGRRIYEAMADGTLGGFDSFQMEGQLLALANYQGACER
                     IKNTPLLRQYHFFTKLFLIVFMALLPFSLIADFSKMGAPALMIPVSILVSFVFGVMGK
                     VGEVNEDPFENRITDVPLTALCNTIERDLREMLGETDLPDKLEPKEGFIF"
     assembly_gap    1822..1848
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1849..2130)
                     /locus_tag="PK198_22175"
     CDS             complement(<1849..2130)
                     /locus_tag="PK198_22175"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRF41524.1"
                     /translation="MIVKKIFNPLSVARYMRVELIASTILSAAVFFLYHNRHLEKVSL
                     PFSIAAILGSALAIFLAFRNNNSYSRWWEARTVWGGIVNSSRVLARLIIT"
     gene            complement(2127..2771)
                     /locus_tag="PK198_22180"
     CDS             complement(2127..2771)
                     /locus_tag="PK198_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRF41525.1"
                     /translation="MDFNKIFQNNQLWIAEKLAADPHYFETLAKGQTPEFLYIGCSDS
                     RVTAEDLMGLQPGEVFIHRNIANQVVPTDNNVNAVIQYAVEHLKVKHIIVCGHYECGG
                     VKAALHPSDMGQLNSWLQTLRDVRRLHRQELEAIDDKTKLFDRLVELNVLEQCTNIIK
                     IDHVQRAWYKTGYPKIHGWVFDVRTGKLIDLKLNMEKEFGSIRHIYDLKPLEKQ"
     gene            complement(2799..3410)
                     /locus_tag="PK198_22185"
     CDS             complement(2799..3410)
                     /locus_tag="PK198_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41526.1"
                     /translation="MAAMLLYGGMAAAQSVEAYFGHQRAGVDLMWFSNFKDAQGERSP
                     FLFFSRNRASTNYDHSPTAFGSTNAVSYNFKSGWGLVAVGSYLNAGFRPKAGVQFVKM
                     KGDFLFFGWLVADLKREGNLDLFGMFRWQPKLGKGNLRLFSQLELFPVYHPDNDIWSI
                     TQRVRAGVKDRTWEAGLMADFNQAGKIDFIQTNNLGAFLRHDF"
     gene            complement(3437..>4129)
                     /locus_tag="PK198_22190"
     CDS             complement(3437..>4129)
                     /locus_tag="PK198_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRF41527.1"
                     /translation="VASSPATSQQTGWPIGFWAAELTHPLRVFQEAGYEVELVSTEGG
                     KLMMDGYSDPADPSGYSAHDVISRGYLQQQWFNDMLDNTKKLSDVNPADYVAVFLVGG
                     QGPMYTFRGNQELEKVFAHFYESGKPSAAVCHSTTLLLEAKTSNGELLVKGKTWTGFA
                     DAEEEFADQAVGMKIQPYRIETEARKIAGTTFKVAAPFSSYAIQDGNLITGQQQNSGA
                     AAAELVVKALQQ"
     assembly_gap    4130..4169
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4342..>4639
                     /locus_tag="PK198_22195"
     CDS             4342..>4639
                     /locus_tag="PK198_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF41528.1"
                     /translation="MPDFIYKGKVYYNPVQLVMEQIGGIWKMPILWRLKDQTMRYSEL
                     RRIPHISDKMLTAQLRELEADGYIHREVYPVVPPKTEYSLTEKGREVIPLIHQIR"
BASE COUNT         1189 a         1197 c         1127 g         1032 t
ORIGIN      
        1 ggtctttacc atccgattgc ccgtcgcagc aaagagtccg caatcttgaa tccgcaatct
       61 tgaatccgaa atcaggaatc cgaaatcagg aataaaaaaa cgctatggaa aaaacataca
      121 tcatctgcgt agaggaccaa cgcgaagtac tcaatacagt actgaaagac ctcgaaatat
      181 ttgaaggccc gttcttcctc gaaggctgcg agagtgccga cgaagcggaa gagctgttgg
      241 atgagctgga cgctcagggc gactatgtgg gcctcatggt atgcgaccac gtcatgcccg
      301 gcaaaagcgg ggtgcaattg ctcatagacg tgaacaaaga cgggcgcttt cggcacacca
      361 aaaaactgct gctcaccggc ctggctaccc atcaggacac gatagaagcc atcaatcagg
      421 cggccattga ccgctacatc gaaaaaccct ggaacgtgga aatcctccaa aaacaggccc
      481 gcatcctgct cacccgcttc ctgtttgatc agggcctgaa ctacgaaccg tttctgccgg
      541 tattggataa ggaaactgta ttggagcggc tgcgacggat ggggtgagag gtgattcatc
      601 gcctgctcag tcgctccggc aaaaaatacc aaaccggaat accgataaag ataatgaaag
      661 acagatacac agaaacgaag gccagcagca ccgatgacaa atagagcaaa gtggatacca
      721 cattgctcct gtgaaccacg gcactgtaag gaatgagttg gccttctatt gccgcatacc
      781 gcctcagcaa taaaaagcca aatgcattca aaaacatcac caagccataa aacatgaccg
      841 cctgcggtaa aaacggatga gccgccagaa agtgcgtggg catggggatc aaagacatgg
      901 caaacagcaa aaacatattg taccatacca agcggggcgt ggaatggggt attctttcaa
      961 ataaaacatg atggttgagc cacataatgg ccagtaccat aaaactcaaa atgtaagaca
     1021 acaccttggg taccaccata cggagcgcat tccacacgtc ttgatcagaa aaaccgggac
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnncag atcaaatgcc atgatggtga tgataatggc
     1141 aatgacgccg tcgctgaatg cctcgattct gtttttggac aacatattct aaaaaataaa
     1201 cccttccttc ggctccagct tgtccggcaa atccgtttca cccagcattt cccgcaaatc
     1261 ccgctctatc gtattgcaca gggccgtgag cggcacgtct gtaatccggt tttcaaaggg
     1321 gtcttcgttc acctcgccga ctttgcccat cacgccaaag acaaacgaca ccagaatact
     1381 gacaggaatc atcagtgccg gcgcgcccat tttgctgaaa tcggcgatga gcgaaaacgg
     1441 cagcagggcc ataaaaacga ttaaaaacaa cttggtaaag aagtgatatt gccgcagcag
     1501 cggcgtgttt ttgatgcgtt cgcaggcgcc ctggtagttg gctaaggcca atagctgtcc
     1561 ctccatttgg aaactgtcaa aaccgcccag cgtaccgtcg gccatagcct cgtaaatccg
     1621 ccgccctgtc agcaattgca gggcattggg ctggtttttg gatgcagcca ccgaacggaa
     1681 agcggcttcc gaaagcaagg gccgcagcgc ttcccagtcg tcctgcctgc gcaaatgcag
     1741 gcgcagggcg tgcccccagg caatgagcaa atacaccatt tcttttttga aggcttcgct
     1801 ccgctctttt acatactggg gnnnnnnnnn nnnnnnnnnn nnnnnnnngg tgatgatgag
     1861 gcgcgccagc acccggctgg agttgacgat gccgccccac accgtgcggg cctcccacca
     1921 acggctgtag gagttgttgt tgcgaaatgc cagaaaaatg gccaaggcac tacccagaat
     1981 agccgcaatg gaaaacggca ggcttacttt ttccaaatgc cggttgtggt acagaaaaaa
     2041 tacggctgcc gacaatatcg tggatgcgat caattcgacg cgcatgtagc gcgccacaga
     2101 cagcggattg aatatttttt tgacgatcat tgtttttcca atggctttaa gtcataaata
     2161 tgccggatcg aaccgaattc tttttccatg ttcagtttca gatcaatgag cttgccggtg
     2221 cgtacatcaa acacccagcc atgaattttg gggtatccgg ttttgtacca ggcccgctgt
     2281 acatgatcta tttttatgat gttggtgcat tgttccaaca cattgagttc tactaagcga
     2341 tcgaacagtt tcgtcttatc gtcgatggct tccagttctt gcctgtgtaa acggcgcaca
     2401 tcccgcagcg tttgcagcca gctgtttaat tgccccatgt cgctggggtg cagggctgcc
     2461 ttcacgccgc cgcactcgta gtgcccacac acaatgatat gcttgacttt cagatgctcc
     2521 accgcatact gaatgacggc attgacgttg ttgtcggtag gaacgacttg attggcaata
     2581 ttccggtgaa taaacacttc gcccggctgc aagcccatca gatcttctgc tgttacccgg
     2641 ctgtcggagc atccgatgta caggaattcc ggcgtttgcc ctttggctaa ggtctcgaaa
     2701 tagtgggggt cggctgcgag tttctcggca atccaaagtt ggttgttttg aaatatctta
     2761 ttgaaatcca tatcggtatt tttaagcaaa aatgcacatt aaaagtcatg acgtagaaat
     2821 gcgcccaaat tatttgtttg aataaaatct attttgcccg cctgattgaa atccgccatc
     2881 aggcccgcct cccaggtgcg atctttgaca cccgccctta cacgctgggt gatgctccaa
     2941 atatcgttgt cgggatgata aaccgggaac aactccaact gcgagaacag ccgcaggttg
     3001 cctttaccca gcttcggctg ccagcgaaac atgccgaaca aatcgaggtt tccttcccgc
     3061 ttcaggtcgg ccacgagcca gccgaaaaac agaaaatcgc ccttcatttt tacgaattgc
     3121 actccggctt tgggcctgaa acctgcattc agataggagc caaccgccac taagccccaa
     3181 ccgcttttga aattgtacga cacggcattg gtagagccga aagccgtcgg cgagtggtcg
     3241 taattggtgc ttgctcggtt gcggctgaaa aacaaaaagg ggctgcgctc accttgggcg
     3301 tctttgaaat tgctgaacca catcaggtcc acgcctgccc gttgatggcc gaagtaggct
     3361 tccaccgatt gggccgcggc catacccccg tacaggagca tggccgccaa aaccgcacat
     3421 tttgtaacaa caagcatcat tgttggagtg cttttaccac caactccgcc gccgccgcgc
     3481 ccgaattttg ctgctgtccg gtaatcaggt tgccgtcctg gatagcgtaa gacgaaaacg
     3541 gcgccgctac tttgaacgtg gtaccggcta tttttctcgc ttccgtttcg atgcggtacg
     3601 gctggatttt catgcccacc gcctgatcgg caaattcctc ctctgcatcg gcaaagcccg
     3661 tccaggtttt gcccttcacg agcagctcgc cgttcgatgt tttggcttcg agcagcaaag
     3721 tggtagaatg acataccgct gccgagggct ttccgctttc gtaaaagtgc gcgaatacct
     3781 tttccagctc ctgattgccc cggaaagtgt acatcggccc ctgaccgcct acgaggaaga
     3841 cggccacata gtcggcgggg ttcacatcgc tgagtttttt ggtattatcc agcatgtcgt
     3901 tgaaccactg ctgttgcagg tagccccgcg aaataacgtc gtgcgccgag tagccgctgg
     3961 gatcggcagg atcggaatag ccatccatca tcagttttcc gccctcggtg ctcacgagtt
     4021 ccacttcata gccggcctcc tgaaatacgc gcaaagggtg cgtcaattcg gcagcccaga
     4081 agccaatggg ccaacctgtt tgctgagaag tagcgggcga actggccacn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgaaaatgga cataagactg aacttcggtt
     4201 atctgactca tcgtttggta atgtgattga gtgaaaaatg atggcgcaaa gttgggggca
     4261 tatacctacc tttgccaaga gtattactta aaagtgcgat acatactttt aagttagtat
     4321 ttgaaaacca aacaattaac aatgccggat tttatataca agggaaaagt gtattacaat
     4381 cccgtccaat tggtgatgga gcaaatcggc ggcatctgga aaatgcccat tctgtggcgc
     4441 ctcaaagacc agacgatgcg atacagcgaa ttgcgccgaa taccgcatat ttcagacaaa
     4501 atgctgacgg ctcaactccg ggaattggaa gcagacggct acattcaccg ggaagtgtat
     4561 ccggtagtgc cgcccaaaac ggagtactcc ctcacggaaa aaggccggga agtaatcccg
     4621 ctgatccatc agataaggg
//
LOCUS       DAORLF010000389         4628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10180_length_4628_cov_1.563962, whole genome shotgun sequence.
ACCESSION   DAORLF010000389 DAORLF010000000
VERSION     DAORLF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_4628_cov_1.563962"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK198_22200"
     CDS             complement(<1..230)
                     /locus_tag="PK198_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF41529.1"
                     /translation="MYRILLVEDEESIREAVKLNLELEHYEVVATGSGREAMAIVQEQ
                     RFDLLLLDVMLPEVSGFEICEQVRLTDREVPIL"
     assembly_gap    257..323
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(435..>616)
                     /locus_tag="PK198_22205"
     CDS             complement(435..>616)
                     /locus_tag="PK198_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF41530.1"
                     /translation="KSRIFDKFYRIGNEDTRQTKGTGLGLYIVDQIVKAHGGSISVRD
                     HQPAGSVFLILLPLK"
     assembly_gap    617..720
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<721..1435)
                     /locus_tag="PK198_22210"
     CDS             complement(<721..1435)
                     /locus_tag="PK198_22210"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF41531.1"
                     /translation="MTRDAKLRWGSIIVIGYIMAAFVWWALLLFKKNHIAFEAQYRAG
                     FYEMLAQGLVENEGQYRQSDLYKMLFTTYRKQMFMIIGEGCVFVLSLILGVWFIHNGY
                     RKEVAAGRQRRNFLLSITHELKSPIAGIQLAVETLLKRDLPREKSLPLLQSSLRETTR
                     LKNLVNDLLLSAKLESAYQLHIEPLNLAELAAGIVEDFRIRYPKAGFEFEAPDELPLF
                     SGDRSGLTSVVQNLVENAVK"
     gene            complement(1432..>2037)
                     /locus_tag="PK198_22215"
     CDS             complement(1432..>2037)
                     /locus_tag="PK198_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRF41532.1"
                     /translation="WMHPGSKLLFMGGEFGQTTEWSIEKGIQWSLLDKPGHRGVQLWV
                     QQLNKFYTAHPALYRKQFSHEGFEWIDLQDYKNSVLVFLRKGDKTDEQLIVALNFTPV
                     PREGYHVGAPAGGVWTEVLNSDAAEWGGSGKYENGKAKAEKAPRHGRDYSLALTLPPL
                     GCVVLLQKPAPRKTSAKAVPAAEKAAEPASPKPKTAKKKAG"
     assembly_gap    2038..2055
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2056..3437)
                     /gene="glgB"
                     /locus_tag="PK198_22220"
     CDS             complement(<2056..3437)
                     /gene="glgB"
                     /locus_tag="PK198_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRF41533.1"
                     /translation="MSKVIEHSLLTDFDVSLFRAGKHFRIYNKLGSHITEVEGQSGVY
                     FGVWAPNARNVSVIGSFNGWNPQAHPLAPRHDGSGIWEGFIPGAKKGDLYKYRIVSHD
                     GRRLEKTDPYGRFFEIPPNTATVIWDNAYEWKDDVWMERRRKNAGLQQPFSVYEVHLG
                     SWMKKSGGFESLTYRELAEKLPAYVREMGFTHVEFLPVMEHPYFPSWGYQITGYFAPS
                     SRFGTPQDYMHLVDALHQAGVGVILDWVPSHFPTDAHGLADFDGTHLYDHADPRKGFH
                     PDWKSAIFNYGRHEVRSFLISNALYWMDAYHTDGLRVDAVASMLYLDYSRKEGEWLPN
                     HLGGKENLEAIGFLKEFNETVYSEYPDTMTIAEESTAWPGVSKPTFDGGLGFGQKWMM
                     GWMHDTLKYFLTDPLFRSHNHNAITFSIVYAFSEHFMLPLSHDEVVHGKGPLIDRMPG
                     DHWQRLANLRL"
     assembly_gap    3443..3483
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3711..>4628
                     /locus_tag="PK198_22225"
     CDS             3711..>4628
                     /locus_tag="PK198_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41534.1"
                     /translation="MKKAIITILGVIFLLNFTLANDQISEAEKLALTGKVWGFLKYYH
                     PNVAAGKFNWDEELFRILPKVKAIGTKEDLSQVYLEWIQGLGRVKECKKCKTVGKQEI
                     FDENFNLNWINNSQILTSAVIKELLFIEQNRHQGKKHYVTTYKKVGNINITNETIYKN
                     FDWTNENLRLLTLFRYWNIVEYFFPYKYQTDTNWDEVLISKFSHPKTELDFHLAMLEL
                     IVSIDDSHAGLYTDVMTDFFGKYMMPVYCEIIEDKVVVTHFFNEALAEKDDLRIGDVI
                     TKIDGQSLEDIFFNRSKYITGSNIPRKRHN"
BASE COUNT         1128 a         1087 c         1102 g         1081 t
ORIGIN      
        1 agaatcggca cttcgcggtc ggtgaggcgc acctgctcgc agatttcaaa accgctcact
       61 tcgggcagca tcacatcgag caaaagcaag tcgaaacgct gttcctgcac aatcgccatg
      121 gcctcccggc cgctgccggt ggccaccacc tcgtaatgtt cgagttccag attcaacttc
      181 accgcctcgc ggatgctttc ttcgtcttct accaatagga ttcggtacat gttgttcaag
      241 gttgatttat agttgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnccaaaag taaaaaattc attttcaaaa tcacattcag
      361 aaatttcccc ttgaggaaaa tcaaattcct tagaaaaaac aagctaatgg cttatggctt
      421 gcagcccaaa tagattattt caaaggcaac aaaatcaaaa aaacagaccc cgccggctga
      481 tgatcccgca ccgaaatact gcctccgtgc gccttcacaa tctgatccac tatgtacaaa
      541 cccaaacccg tgcctttggt ttgccgcgta tcctcgttgc cgatgcggta aaacttgtcg
      601 aaaatgcgcg atttctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 acttgacggc attttcaacg agattctgga caacggaggt aagcccggag cggtcgccgg
      781 agaacaaggg caattcatcg ggcgcttcaa actcaaagcc ggccttgggg tatctgatgc
      841 ggaagtcctc cacaataccg gcggcaagct cggccagatt caagggttcg atgtgcaact
      901 ggtacgccga ttcgagcttg gccgagagca gcaggtcgtt caccaggttc ttcagccgcg
      961 ttgtttcgcg cagggagctt tgcagcaaag gcaggctctt ttcgcggggc aaatcccgtt
     1021 tcaacagcgt ttccaccgcc agttgtatgc ccgctatggg cgacttcagt tcgtgggtga
     1081 tggagagcag gaaattgcgc cgttgccggc ccgcagccac ttctttccgg tagccgttgt
     1141 ggatgaacca cacgcccaaa atcaggctga gcacaaacac acaaccctcg ccgatgatca
     1201 taaacatctg cttgcgatac gtcgtgaaga gcatcttgta caggtcgctt tgccggtact
     1261 gcccttcatt ttccaccaaa ccctgggcca gcatttcata aaaaccggcc cggtattgcg
     1321 cctcgaatgc aatgtggttt tttttgaaca acaacaatgc ccaccacaca aaggccgcca
     1381 tgatgtagcc gatcacaatg atggaacccc accgcaattt ggcgtcgcgc gtcatcccgc
     1441 tttctttttg gccgttttgg gtttgggcga agccggttca gccgcttttt cggcagcagg
     1501 cacagctttg gcgcttgttt tgcgcggcgc cggtttttgc agcagcacca cacagcccaa
     1561 aggcggcagc gtcagagcga gggagtaatc gcggccatgt cgcggcgctt tttcagcctt
     1621 cgctttgccg ttttcatact tgcccgaacc gccccattcg gctgcatcgc tgttcagcac
     1681 ttccgtccaa acgccgcccg ccggagcgcc gacgtggtag ccttcgcggg gtacgggcgt
     1741 gaaattgagc gccacgatga gttgttcgtc ggtcttgtcg cctttgcgca agaagacgag
     1801 tacgctgttt ttataatcct gtaaatcaat ccattcgaag ccctcgtgcg aaaattgctt
     1861 gcgatagagg gccggatgag cggtgtaaaa tttgttgagt tgctgcaccc agagctgtac
     1921 tccgcgatgt cccggcttgt ctaacaatga ccactgaatg cctttttcga tgctccattc
     1981 ggtggtttgc ccaaactcgc cgcccatgaa cagcaatttg ctgccggggt gcatccannn
     2041 nnnnnnnnnn nnnnnaggcg caggttggcc aagcgctgcc agtgatcgcc gggcatgcgg
     2101 tctatcagcg ggcctttgcc gtgtaccact tcatcgtgcg aaagcggcag cataaaatgc
     2161 tcgctgaaag catacacgat gctgaacgtg atggcattgt ggttgtggct tctgaacagc
     2221 ggatcggtga ggaaatactt gagcgtgtcg tgcatccagc ccatcatcca tttttggccg
     2281 aagcccagac cgccgtcaaa agtaggcttc gaaacgcccg gccaggccgt tgactcctcc
     2341 gcgatggtca tggtgtcggg gtattcggaa taaacggtct cgttgaattc cttcaaaaag
     2401 ccgatggcct ctaagttttc cttgccgccg agatgattgg gcagccattc gccctctttt
     2461 ctggaataat ccaaatacag catcgaggcc accgcatcca cccgcaagcc gtcggtatgg
     2521 taggcatcca tccagtacaa agcgttggaa atgaggaaag aacgcacctc gtggcgacca
     2581 taattaaaaa tggcgctctt ccagtcgggg tgaaaaccct tgcgcggatc ggcatgatcg
     2641 tacaggtgcg tgccgtcgaa atcggccaga ccatgcgcat ccgtcgggaa atgcgagggc
     2701 acccagtcca aaatcacgcc cacgccggcc tgatgcaggg catccaccaa gtgcatgtag
     2761 tcctgcggcg tgccgaaacg gctgctcggc gcaaaatacc cggtgatctg atagccccag
     2821 gacgggaaat aggggtgctc catgaccggc aaaaactcga cgtgcgtaaa gcccatttcg
     2881 cgcacatacg ccggcagttt ttcggccaac tccctgtatg tcagcgattc aaagcctccg
     2941 ctttttttca tccaggagcc gaggtgtacc tcatacacgg aaaacggttg ctgcaaaccc
     3001 gcatttttcc tgcgccgctc catccagaca tcgtctttcc attcgtaggc attgtcccat
     3061 atcacggtgg cggtattggg cggaatttca aaaaatcgcc cgtaagggtc cgttttctcc
     3121 agacggcgac cgtcgtgcga tacgatgcgg tatttgtata aatctccctt tttggcgccg
     3181 ggaataaagc cttcccagat gccggagccg tcgtgccggg gcgcgagggg atgggcctgc
     3241 gggttccagc cgttgaaact gccgatgacg gacacattgc gggcattggg cgcccacacg
     3301 ccgaaataaa cgcccgactg cccctccact tcggtgatgt ggctgccgag tttgttgtaa
     3361 atcctgaagt gctttccggc ccgaaacagg ctgacgtcaa agtcagtgag caggctgtgt
     3421 tcgattactt ttgacatacg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnncattgcc gccattgtgg aggcaggaat gtggaggcgc gacgaaaagc ggagcgaggg
     3541 ggcgaaaagt ttttttaggc agcagtcaat tttatgccaa acttacatta gctttgcaga
     3601 gttgagttcg gaatggagac ctgtgagaaa agtggatgcg gggggattga cgacagggag
     3661 gctatgggcc gagagttcgg taaacataag ttaacaacca tataaagaaa atgaagaaag
     3721 caattatcac tatacttgga gtaatatttc ttttaaattt tacccttgcc aatgaccaga
     3781 tttctgaagc agaaaaatta gctttaactg ggaaggtttg ggggttccta aaatactacc
     3841 atcctaatgt tgctgctggt aaatttaatt gggatgaaga attatttagg attttgccta
     3901 aagttaaagc catcggcaca aaggaagatt tatcccaagt gtatcttgag tggattcaag
     3961 gattaggacg agtgaaggaa tgtaaaaaat gtaaaacagt aggcaaacag gagatttttg
     4021 atgaaaattt caatctcaac tggataaata attcgcagat acttacttct gccgtgatca
     4081 aagaactatt gttcattgaa caaaatagac atcaaggaaa gaagcattat gtaacaactt
     4141 ataagaaagt aggcaatata aatataacca atgaaactat ttataaaaac tttgattgga
     4201 caaatgaaaa tttacgattg ctgacgcttt tccgttattg gaatattgtc gaatacttct
     4261 ttccctataa atatcaaact gatacgaact gggatgaggt attaatttct aaattttcgc
     4321 acccaaaaac tgaactggat ttccatttgg caatgctgga attgattgtg agcattgacg
     4381 atagtcatgc agggttatac acagatgtga tgacagattt ttttggtaaa tacatgatgc
     4441 cggtttattg tgaaataatt gaagacaaag tagttgttac tcattttttc aatgaagctc
     4501 ttgccgaaaa agatgacttg cgaataggcg atgtaatcac aaaaatagat ggtcagtcat
     4561 tggaagatat ttttttcaat aggtccaaat acattacagg ttcaaatatt ccaagaaaaa
     4621 ggcataat
//
LOCUS       DAORLF010000390         4625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10191_length_4625_cov_1.987309, whole genome shotgun sequence.
ACCESSION   DAORLF010000390 DAORLF010000000
VERSION     DAORLF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10191_length_4625_cov_1.987309"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PK198_22230"
     CDS             complement(<1..982)
                     /locus_tag="PK198_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRF41535.1"
                     /translation="MSLLNRINAELKGDRVIWAILAVLSVFSILVVYSSTGTLAWRER
                     GGDTEAYLFKHTMILAMGLALAYLAYLMHYRRYAKIAPILLLAAVPLLLLTIMFGAEI
                     NDARRWIVIPGLGLSFQTSDFAKLALIVYVAREISSKQDYIKDWKQAFVPIIVPVLIV
                     CGLIAPANLSTAVLLMVVCIGMMFVGRVSLKYIGLLLLLGVVLFSALIFLGEFFPNYI
                     RVDTWTSRITDFIENPQGSFQIQQAKIAIANGEWIGNGPGNSIQRNFLPSPYADFIYA
                     VICEEYGFVGGALIVVLYVVLFFRVTRLVTKSPKAFGAMMATGLTLLLVMQ"
     gene            complement(987..2309)
                     /gene="murD"
                     /locus_tag="PK198_22235"
     CDS             complement(987..2309)
                     /gene="murD"
                     /locus_tag="PK198_22235"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRF41536.1"
                     /translation="MKIAILGAGESGTGAALLAKAKGHEVFVSDLGAIQDHFKRELES
                     EGIEYEEGKHTWERILTAEEVIKSPGIPDKAPLVKALRENGIPVISEIEFAARYSNTF
                     TIGITGSNGKTTTTNLTWHLLHQAGLKAGLGGNVGYSFARLVLEGNYDYFVLELSSFQ
                     LDGVLKFRPDIAMLLNITPDHLDRYEYKMENYVASKFRIAMNQTESDLFIFNADDANM
                     SEYMSRHAIAARRAPIGESLIEDTRIQVGNSVFDMNETPLQGRHNYMNALFAVQTAQA
                     LGLSEDAIRAGLKSFINVPHRMERITEINGVQYINDSKATNVDAVFYALDAMTAPVVW
                     IVGGQDKGNDYEPLRQLVRQKVRAIVCLGVDNAKIVEFFGADVADITETNSAETAAYA
                     AMQLAQPGDAVLLSPACASFDLFRNYEDRGDQFREAVLKIKSEKTTKS"
     gene            complement(2316..>2665)
                     /locus_tag="PK198_22240"
     CDS             complement(2316..>2665)
                     /locus_tag="PK198_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRF41537.1"
                     /translation="GFLWYNAFPAQVFMGDTGSLTLGGIIAALAIVLRKELLIPLLCG
                     IFVVENLSVMLQVAYFKYTKKKYGEGRRIFKMSPLHHHYQKLGMHESKIVTRFWIVGI
                     MLAVITVITLKIR"
     assembly_gap    2666..2813
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2814..3584)
                     /locus_tag="PK198_22245"
     CDS             complement(<2814..3584)
                     /locus_tag="PK198_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRF41538.1"
                     /translation="MLINLIEWLQKLFGDIPGAGLFQYITFRAGLAVILSLIISMLFG
                     GRIIRFLRQLQIGETVRDLGLDGQKQKEGTPTMGGLIIIMAILIPSLLVAKLDNVYII
                     LMIISTIWMAVIGFVDDYIKVFLKNKEGLSGKFKILGQVGLGLIIGITMMMSDDVTVR
                     MDTETAEHIGFSESQMVQGPFYIGEGNTVAEKADYKASLTNVPFLKGNRFDYAWVLPM
                     EESKAERWVWLLVIPLVIFIVTAVSNAANLTDGIDGLAT"
     gene            complement(3596..>4625)
                     /locus_tag="PK198_22250"
     CDS             complement(3596..>4625)
                     /locus_tag="PK198_22250"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRF41539.1"
                     /translation="DAVAINALLAEMVAQGCAFAFMEVSSHALEQKRVEGLQFAGAMF
                     TNITHDHLDYHKTFDNYIRAKKLLFDGLGKDTFALVNVDDRRGMVMVQNTKAKVWRYA
                     LRSMADFKAKILENTLEGLHLELDGFDFYGRLVGEFNAYNLLAVYAAAVLLGHDKSEV
                     LTALSRMQAAEGRFDLVSSRERGITAVVDYAHTPDALEKVLETLTKLRGAGRIITVTG
                     AGGDRDKAKRPVMAKTACDWSAQVILTSDNPRSEKPEDILADMQQGVPITAAAKVLTI
                     TDRRQAIRTACAIAQPGDIILVAGKGHEKYQEIQGVKYPFDDKEELRIALEIRNPKSG
                     IRNQESES"
BASE COUNT          997 a         1227 c         1193 g         1060 t
ORIGIN      
        1 cctgcatgac caacagcagc gtcaggccgg tagccatcat ggcgccgaaa gctttggggc
       61 ttttggtcac caagcgcgtg acgcggaaaa acagcaccac atacagaacg acgatgagcg
      121 ctccgcctac aaaaccatac tcctcgcaga tgacggcgta aatgaagtcg gcgtagggcg
      181 aaggcaggaa gttacgctgt atgctgttgc cggggccgtt gccgatccat tctccgttgg
      241 caatggcaat tttggcttgc tggatttgaa aactaccttg tgggttttct atgaaatcgg
      301 tgatgcgaga agtccaggta tctacccgaa tatagttggg aaaaaactct cccaaaaaga
      361 tgagtgccga aaacagcacc acccccagca acaacagcaa gcctatgtat ttgagcgata
      421 cccgccccac gaacatcatt ccgatacaaa ctaccatcag cagtaccgct gtggacaagt
      481 tggccggcgc aatcagcccg caaactatca gcaccggcac gatgatgggc acaaaagctt
      541 gtttccagtc tttgatgtaa tcttgtttgg acgaaatctc gcgggccaca tacacgatga
      601 gcgccagctt ggcaaagtcg gaggtttgga agctcaagcc caagccggga attacaatcc
      661 agcggcgggc atcgttgatc tcggcgccga acatgatggt gagcaacaac aagggcacgg
      721 ctgccaacag caaaatgggc gctattttgg cataccgccg gtagtgcatc aggtaagcca
      781 gataagcgag cgcgaggccc attgccaaaa tcattgtgtg cttgaacagg tatgcctcgg
      841 tgtcgccgcc gcgctcgcgc caggccagcg taccggtaga actgtacacc accaggatgg
      901 aaaagaccga caatacggcc aggatggccc atatcacccg gtcgcccttc agttcggcat
      961 tgattctgtt caataatgac atagcgttat gatttcgtag ttttctctga tttgattttc
     1021 aaaacggcct cccgaaactg atcgccccgg tcttcgtagt tgcgaaacag gtcgaaactg
     1081 gcgcaggccg gcgacaagag caccgcatcg cccggctggg ccaattgcat cgcggcataa
     1141 gcggccgttt cggcgctgtt cgtctctgta atgtcggcca catcggcgcc gaaaaactcg
     1201 acgattttgg cattgtccac acccagacac acaatagcgc gcaccttttg gcgcaccaac
     1261 tgccgcagcg gttcgtagtc gttgcctttg tcctgaccgc ccacaatcca cacaaccggc
     1321 gcagtcatgg cgtccaaggc gtaaaaaaca gcgtccacgt tggtggcttt gctgtcgttg
     1381 atgtactgca cgccgttgat ctcggtgatg cgctccatgc ggtgcggcac gttgatgaag
     1441 ctcttcagcc cggcccggat ggcgtcctcg ctgaggccca gcgcctgagc ggtttgcacg
     1501 gcaaagaggg cgttcatgta gttgtggcgg ccttgcaacg gcgtttcgtt catatcgaaa
     1561 accgagttac ccacttggat gcgcgtgtcc tctatcaagc tttcgccgat gggtgcgcgg
     1621 cgggctgcaa tggcatggcg cgacatgtac tcgctcatgt tggcatcatc cgcgttgaaa
     1681 atgaacagat cgctttcggt ctgattcatg gcgatacgga acttcgacgc cacataattt
     1741 tccattttgt actcgtagcg gtcgaggtgg tcgggggtga tgttgagcag catggcgatg
     1801 tcggggcgga atttcaacac gccgtccaat tggaagctgc tgagttccaa cacgaaatag
     1861 tcataattgc cttccaaaac caagcgggca aaactgtagc ccacattacc gcccaaacct
     1921 gccttcaaac ccgcttgatg cagcaggtgc caggtgaggt tggtggtggt ggttttgccg
     1981 ttgctgccgg tgatgccgat ggtgaaggtg ttggaatacc gggccgcgaa ctcgatttcg
     2041 gagatgaccg gaatgccgtt ttcgcgcagg gctttcacca atggcgcttt atcgggtatg
     2101 cccggacttt tgatgacttc ctctgccgtc agtatgcgct cccaggtgtg cttaccttcc
     2161 tcgtattcaa tgccttctga ctctaattca cgtttgaagt gatcctgaat agcaccgaga
     2221 tcagacacga atacctcgtg tcctttggct ttagcgagca gggccgcccc cgttccgctt
     2281 tctccggctc ccagaatggc tattttcatc gttacttatc tgatctttaa cgtgatgacg
     2341 gtaatgaccg ccagcataat tcccacaatc cagaagcgcg ttacaatctt gctctcgtgc
     2401 atccccagct tctggtaatg gtgatgcaaa ggcgacattt tgaaaatgcg acggccttcg
     2461 ccgtacttct ttttggtgta tttgaaataa gctacctgca acatcacgga gagattttcc
     2521 accacaaaga tgccgcacag cagaggaatg agcagttctt tgcgcaacac aatggccaac
     2581 gctgcaatga tgccgcccaa tgtgaggctg ccggtatcgc ccatgaacac ttgtgccgga
     2641 aaggcattgt accacagaaa tccgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgttgcc
     2821 agcccgtcta tgccgtcggt gagattggcc gcgttggata ccgccgtgac gatgaaaatg
     2881 accaacggta ttaccaacag ccatacccag cgctcggctt tgctctcctc catcggcagc
     2941 acccaggcgt agtcaaatcg gttgcctttc agaaagggca cattggtgag gctggctttg
     3001 tagtcggcct tttccgctac cgtattgcct tcgccgatgt aaaacgggcc ttgcaccatc
     3061 tggctttcgc tgaatccgat gtgctcggcg gtttctgtat ccatgcgcac ggtcacgtcg
     3121 tcgctcatca tcatggtgat gccgatgatg aggcccaaac ctacctggcc caggattttg
     3181 aacttgcccg acaagccttc tttgtttttg agaaagactt tgatatagtc gtccacaaac
     3241 ccgatcacag ccatccaaat cgtggaaatg atcatcagaa tgatgtaaac attgtccaat
     3301 ttggccacca acaggctggg aatcaatatg gccatgatga tgatgaggcc gcccatcgta
     3361 ggcgtaccct ccttttgttt ctggccgtcg aggccaagat cgcgaactgt ttcgccgatc
     3421 tgcaactgcc tcaaaaaacg gatgatgcgg cctccgaaca acatcgaaat gatgagcgag
     3481 aggatgaccg ccaatccggc acgaaacgtg atgtattgaa acaatcccgc tccgggtatg
     3541 tcaccgaaca gcttttgcag ccattctatg aggttgatga gcatacgttg tttttttagc
     3601 tttcggattc ctgattcctg attcctgatt tcggatttcg gatttcgaga gcaatgcgca
     3661 gttcttcttt atcgtcgaaa gggtacttta ccccttgaat ttcctggtat ttttcgtggc
     3721 ctttgccggc taccaaaatg atgtctccgg gttgggcgat ggcacaagcg gtgcgaatgg
     3781 cctgccgtct atcggtgatg gtgagcacct tggcggcggc ggtaatgggt acgccctgct
     3841 gcatgtcggc gaggatgtct tccggttttt ccgaacgcgg attgtcggaa gtgagaatca
     3901 cctgagcgct ccagtcgcag gccgttttgg ccatcacggg ccgtttggcc ttgtcgcggt
     3961 cgccgccggc gccggtcact gtgatgatgc gccccgcgcc gcgcagtttg gtcagcgttt
     4021 ccagcacttt ttcgagtgcg tcgggggtgt gcgcataatc cactacggcg gtgatgcccc
     4081 gttcgcggga actgacgaga tcgaaacgac cttcggcagc ctgcattcgg ctgagcgcgg
     4141 tgagcacctc cgatttgtcg tgccccaaca gcactgcagc agcatacacg gctaagagat
     4201 tgtaggcatt gaactctccg accaagcgac cgtaaaaatc aaaaccgtcg agttccagat
     4261 gcagcccttc gagggtgttc tccagaattt tggctttgaa atcggccatg ctgcgcaggg
     4321 catagcgcca gactttcgct ttggtgtttt gcaccatcac catgccgcga cggtcgtcca
     4381 cgttgaccaa tgcaaaggta tccttaccca atccgtcaaa gagcagtttt ttggcgcgga
     4441 tgtagttgtc gaaggtcttg tggtagtcca aatgatcgtg ggtgatgttg gtaaacatag
     4501 cccctgcaaa ctgcaaacct tccacccgct tctgctccaa cgcgtgcgaa ctcacctcca
     4561 taaaagcaaa agcgcagcct tgtgctacca tttcggccag cagcgcattg atggctacgg
     4621 catcg
//
LOCUS       DAORLF010000391         4617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10208_length_4617_cov_1.669005, whole genome shotgun sequence.
ACCESSION   DAORLF010000391 DAORLF010000000
VERSION     DAORLF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10208_length_4617_cov_1.669005"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1084)
                     /locus_tag="PK198_22255"
     CDS             complement(<1..>1084)
                     /locus_tag="PK198_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF41540.1"
                     /translation="YSVAPAPKPQEGYYHNTEDYSPIAENRFHKATDTPLSTFSIDVD
                     AASYSNMRRFLKNGQMPPADAVRIEEMINYFDYQYPQPTGEHPFEVVAEMSECPWQPE
                     HRLLHIGLKGREIATDKLPPSNLVFLIDVSGSMDSPNKLPLLQSAFRLLVNQLRPQDK
                     VAMVVYAGAAGLVLEPTDGNDKQKILEALQRLQAGGSTAGGAGIRLAYDVARQHFVKG
                     GNNRVILATDGDFNVGASSDADMVRLIEEERKSGVFLTVLGFGMGNYKDNKMQQLSNA
                     GNGNHAYIDDIDEAKKVLVEEFGGTLFTIAKDVKIQIEFNPAQVAGYRLIGYENRLLN
                     KEDFNDDKKDAGELGAGHTVTALYEIV"
     assembly_gap    1085..1099
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1100..1419)
                     /locus_tag="PK198_22260"
     CDS             complement(<1100..1419)
                     /locus_tag="PK198_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41541.1"
                     /translation="MKPPHTIHGIVSDDNGDPLIGASVLVKGTTLGTVTDLEGRFSLT
                     TQDSCATLVISYTGYETQEIKKACASKPIRVKLAPSNVSLEEVVVLGGNRPSKKERSA
                     AVQSV"
     assembly_gap    1732..1842
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1843..2153
                     /locus_tag="PK198_22265"
     CDS             <1843..2153
                     /locus_tag="PK198_22265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF41542.1"
                     /translation="AKNHCLMALRKAGRHFLQSSDPALMQSDAFWHPDMEGDEDAVLA
                     RLQKCMEQLTAQQKRCIDLFYLQGKSYKDIADEDGEELGKVRSHIQNGRRNLKNCMEK
                     "
     assembly_gap    2156..2165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2216..3520
                     /locus_tag="PK198_22270"
     CDS             2216..3520
                     /locus_tag="PK198_22270"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF41543.1"
                     /translation="MQASNNIQNLKDLLRRRIAGHAGAEEERMLSKQAAEDPFLQDAL
                     EGLDAFPEGDHTARLARLQERLERRTRRRIVPIFVQRAAAAVLVLGLFGLGWWLLSDG
                     QEQISGPSAPQAVVIQPADPKPDAPAPVADNTSSQVAPMPKSRPTPATTLPQQPAVLP
                     PLNLPDDLAAAEKPADTAPADASGRAVAAEVAEAMPAPEQKKESIAAPKATDSDMAKS
                     QPARAATPSAKPDAPAPAGFRVITGKISDGDTGDPLIGATVLLQGTQQGTVTDFDGSY
                     RLQIPEKEANARIVVSYTGYETKVVEAGERNKLNVNLDGSLATLSEVVVLGGSKKRKA
                     DRSGAVRSFSADAAPQNGWDALEQHIAKNRRMPVQAVANGISGQVLVEFTVTAKGKLR
                     DFKVLESLGFGCDEEAVRLLQTGPAWRNDTGKEQKATYSVQF"
     gene            complement(3530..4594)
                     /locus_tag="PK198_22275"
     CDS             complement(3530..4594)
                     /locus_tag="PK198_22275"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRF41544.1"
                     /translation="MLSNIKVIELASVLAGPAVGMFFAELGAEVIKVENKTAGGDVTR
                     SWKLPSEDPAAPASAYYASVNWGKTVWMCDLRHADDRLRVLEAIDQADIVISNFRPDA
                     AQKLGMSYAQLSARNPRLLFGQITGFGDEDPLPAFDVVLQAEAGFLYMSGHPGGAPAK
                     MPVALIDLLAAHQLKEGLLLALLRREQTGQGAYVSVSLLESAIASLANQATNWLMAGH
                     IPQPMGSMHPNIAPYGDIFHTADARPLVLAIGNDKQFQALCDVLGLSQLAHNERFVSN
                     AARVRHRQALCDVLASAIRRFHRDELLALLHKAGVPCGGIRSMAEVFEHPLAQAMLLH
                     EPDEQGKPTLRPRTAVFKME"
BASE COUNT          966 a         1208 c         1340 g          967 t
ORIGIN      
        1 acgatttcgt acagcgccgt cacggtgtgg cctgcgccga gttcgcccgc atcttttttg
       61 tcatcgttga aatcctcttt gttcaacagg cgattttcat agccgatgag ccggtagccc
      121 gccacctgcg ccggattgaa ctcgatctgg attttcacgt ctttggcaat ggtgaacagc
      181 gtgccgccga attcctccac caatactttt ttggcctcgt ctatgtcgtc aatataggcg
      241 tggttgccgt tgcccgcatt cgagagttgc tgcattttat tgtctttgta attgcccatg
      301 ccgaagccca gcaccgtgag gaacacgccg cttttgcgct cctcctcaat gaggcgcacc
      361 atgtcggcat cgctgctggc gcccacattg aagtcgccgt cggtggcgag gatgacgcgg
      421 ttgttgcccc ctttgacaaa atgctgccgc gctacatcgt acgctaagcg aatgcccgcg
      481 ccgcccgctg tggagccgcc cgcctgaagc cgttgtaatg cttcgaggat tttttgcttg
      541 tcgttgccgt cggtgggttc caataccaaa cccgccgcgc cggcatacac caccatcgcc
      601 accttgtcct gtgggcgcaa ctgattgact aagagccgga aagcggattg aagcaagggc
      661 agtttgttgg gcgaatccat gctgcccgac acgtcaatga ggaacacgag gttggaaggt
      721 ggcagcttgt cggtggcgat ttcgcggcct ttgaggccga tgtgcagcag gcggtgttcg
      781 ggctgccaag ggcattccga catttcagcg accacctcga agggatgctc gccggtgggt
      841 tgcgggtatt ggtaatcaaa ataattgatc atttcctcga tgcgcacggc atcggcgggc
      901 ggcatctggc cgtttttcag aaagcggcgc atgttgctgt acgaggcggc atccacgtca
      961 atggagaagg tggagagcgg cgtatcggtc gctttgtgga aacggttttc ggcgatgggg
     1021 ctgtagtcct cggtattgtg gtaatagcct tcctgcggtt tgggagcagg tgcaacggag
     1081 taagnnnnnn nnnnnnnnna ctgactgaac agccgctgat cgctcctttt tcgacggtct
     1141 gttgccaccc aaaaccacca cttcctccag cgatacgttg gagggtgcca attttacgcg
     1201 tatcggcttg ctggcgcagg cttttttgat ttcctgcgtt tcgtagccgg tgtaagagat
     1261 gacgagcgtg gcgcaggagt cctgggtcgt caggctgaat cgcccctcca agtcggtgac
     1321 ggtaccgaga gtagtgcctt tgaccaaaac ggaggcgccg atgagcgggt cgccgttgtc
     1381 gtctgatacg atgccgtgaa tggtgtgcgg cggtttcacc gagaagtgcg cgctcagcag
     1441 caggctgatg gcaaaaggac agatttcgtt ttcataatga atgtgttttt cctaaaggat
     1501 gcagggtagg ggaggattac acacggggcg ggatattttt tttaagattc cccccaaaaa
     1561 acctaccttt ctgccctttc attgcacacc atgttccgat ttttccaacg ctccgccgcg
     1621 caggcctccg acgaggaact gctccaacga tattgccgaa agggcgatat ggaggcattg
     1681 ggacttgtgt atcagcggta tatggagttg gtgtacggcg tgtgtctcaa annnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggccaaa aaccactgcc
     1861 tgatggcgct gcgcaaagcc gggcgtcatt ttttgcagag ttccgacccc gcccttatgc
     1921 aatccgacgc cttttggcat cctgatatgg agggcgatga agatgctgta ttggcccgat
     1981 tgcaaaagtg tatggagcaa ctcaccgctc aacaaaagcg ctgcattgac ctgttttatc
     2041 tgcaaggaaa atcttacaaa gacatcgcag acgaagatgg cgaggaattg ggaaaagtgc
     2101 gctcgcatat ccaaaacgga cgcagaaatt tgaaaaattg tatggagaag tagtannnnn
     2161 nnnnnggcga ctttagtcgc ccaaccatcg cgaaagcgat tcactaataa aagacatgca
     2221 ggcatcgaat aacatacaaa acctgaaaga cctcctgcgg cgccgcatcg ccgggcacgc
     2281 cggagcggaa gaggagcgta tgctgtcgaa acaggcagca gaggatccgt ttttgcagga
     2341 tgcgttggag ggcttggatg cattcccgga aggcgatcat acggcccggc tggcaagact
     2401 gcaagagcgc ctcgaacgcc gcactcgccg tcgcatcgtg ccgatatttg tacagcgggc
     2461 cgcggcggct gtgctggtat tgggcttgtt cggattaggc tggtggctgc tttccgatgg
     2521 acaggagcaa atttcagggc catctgctcc tcaggcggtg gtgattcaac ccgctgatcc
     2581 taagcctgat gcgcctgccc ctgttgcaga caacacctct tcgcaagtcg ctccaatgcc
     2641 gaaatcccga ccgactccag ctacgacatt gcctcaacag cctgccgtac ttccgcccct
     2701 caacttgccg gacgacctcg ctgccgccga aaagcccgca gacactgcac ccgcagatgc
     2761 gtcgggtcgg gctgttgctg ctgaagtagc cgaggccatg cctgctcccg aacaaaaaaa
     2821 ggaaagcatt gccgcgccca aagcgaccga ttccgacatg gccaagtctc agcccgcgag
     2881 ggccgccacc ccatccgcaa aacctgacgc gcctgctcct gccggatttc gcgtcatcac
     2941 cggaaaaatt tcagacggag acactggcga cccgctgatc ggcgccacgg tactgctgca
     3001 aggcacgcag cagggcacag tgacggattt tgacggcagc taccgcctgc aaattcctga
     3061 aaaagaagca aatgcacgaa tcgttgtctc ttacaccggc tacgaaacga aggtggtgga
     3121 agcaggcgag cgcaacaaac tgaacgtgaa tctcgacggc tctttggcta cgctcagtga
     3181 agtggtggtg ctcggcggaa gcaaaaaacg caaagcagac agaagcggcg ctgtaagatc
     3241 attttctgcg gatgcagcac ctcagaatgg ctgggatgcc ttggaacagc acattgctaa
     3301 aaaccgccgg atgcccgtac aggctgtggc caatggcata tcggggcagg tgttggtgga
     3361 atttaccgtg acggccaaag gaaaattgcg ggattttaaa gtgctggaat ccttgggctt
     3421 tggctgcgat gaagaggccg tccgactcct gcaaaccggc cccgcctggc gaaacgatac
     3481 cggcaaagag caaaaagcga cttactccgt gcagttttga aaagccccct cactccattt
     3541 taaacacggc agttcgcggc ctcagcgtag gtttgccctg ttcgtcaggt tcgtgcagga
     3601 gcatggcctg tgccaatggg tgctcaaaaa cctcggccat gctgcgaatg ccgccgcagg
     3661 gtacgcccgc tttgtgaagc agcgccagga gttcgtctct atggaaacga cggatggcgg
     3721 aggcgagtac atcgcagagc gcctgccggt ggcgaacgcg ggctgcattg gaaacaaacc
     3781 tttcgttatg agccaattgt gacaagccga gcacatcgca cagcgcctga aactgtttgt
     3841 cgttgccgat ggcgagcaca agcggtctgg catcggcggt gtggaagatg tcgccatacg
     3901 gcgcgatatt ggggtgcata ctgcccatcg gctgcgggat atggccggcc atgagccagt
     3961 tggtagcctg attggccaaa gatgcaatgg ccgattcgag cagcgacacg gaaacataag
     4021 cgccctgccc ggtttgttcg cggcgaagca gggccagcag cagcccttcc ttgagttggt
     4081 gcgccgccag cagatcaatg agcgctacgg gcattttggc cggcgcgccg ccgggatggc
     4141 cgctcatgta cagaaatccg gcttcggctt gcaggacgac atcgaaggcg ggcagtgggt
     4201 cttcgtcgcc gaaaccggtg atctgaccga agagcaggcg cggattgcgg gcgctgagtt
     4261 gggcgtagct catgccgagt ttttgggcgg catcgggccg gaaattgctg atgacgatgt
     4321 ctgcctggtc tatggcttcg agcacgcgca ggcggtcgtc ggcatggcgg aggtcgcaca
     4381 tccacacagt tttgccccaa ttgacggagg catagtaggc cgatgcaggc gcggcggggt
     4441 cttcggaagg gagtttccag gagcgggtca cgtcgcctcc ggcagttttg ttttccactt
     4501 tgatgacttc ggcgcccaat tcggcaaaaa acatacccac cgccgggccg gccagcacag
     4561 aggcaagttc gatgacttta atatttgata acatgatgtt gtttttaatg agtgggg
//
LOCUS       DAORLF010000392         4615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10213_length_4615_cov_2.387500, whole genome shotgun sequence.
ACCESSION   DAORLF010000392 DAORLF010000000
VERSION     DAORLF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_4615_cov_2.387500"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="PK198_22280"
     CDS             <1..1813
                     /locus_tag="PK198_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41545.1"
                     /translation="IIMGIEEDDPNETFEDVMTKLDMLIGLREIKTQVRNHARYIQFL
                     QLRKEKGFEENEGINVHSVFIGNPGTGKTTVAGMMGKLYKKMGLLTKGHVHEVDRVDL
                     VGEYIGQTAPKVREAIERARGGVLFIDEAYSLARSNDDSKDFGREVIEILVRELSNGP
                     GDLAVIVAGYPKEMKHFLASNPGLKSRFNHHFEFRDYLPQELSEIAEFAAREKGIELT
                     PEARKKIDDVIIDAYRRRDRTFGNARFVFDTLEKAKINLGLRVMAMPDPQLLEQERLA
                     RVELYDVAGIGNTERRDLPNIPVDEQLLQESLDEMNRLIGMEKVKAQIKELVWLVRYY
                     RETGKDVLNSFSLHTVLVGNPGTGKTTVARILTKIYKALGMLERGHVVETDRQGLVAG
                     YMGQTAIKTAEKVEESMGGVLFIDEAYALTARIGPNGDFGDEAIQTLLKRMEDHRGQF
                     FVFVAGYPDNMEQFLKANPGLNSRFDKVLKFEDYEPEDLSRIAHIMLAEQNLVPTPEA
                     EAHLKDYLNFVYEYRDKYFGNARTVRGVVQEAVKNQHLRLAALPAEERDATSINILTL
                     EDVAGFRTDKSDAVFTKKTIGFRRASGEGTVGSAAGS"
     gene            1907..2890
                     /locus_tag="PK198_22285"
     CDS             1907..2890
                     /locus_tag="PK198_22285"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRF41546.1"
                     /translation="MNAEQIGKYLSGEASPAEVAELMAWVEQSQANKEFFDNMLQLWA
                     IPEPQPAMPPLNASKAWAALESAIDQRAPAPKNRGIISMKMLLRVAAVLLPLALALWW
                     TLGRNDAPTEVLMAQTIAQERKTITLPDGSTVQLNENSALHYRQDADARYVTLSGEAF
                     FEVKHLDGKPFIIESGEARTTVLGTSFNVRAYPNEERVEVSVSTGKVQVEAIAAPQQK
                     AMLAAGEAVAVQKKDLRLAAAEEIANNATAWKNRQFRFDDTPVDEVVLTLERYFGVDI
                     ALERAAIGACRFSGHYDNPQLEEVLEALSFAIDAQWEQRAEVISLSGNGCR"
     gene            2910..4547
                     /locus_tag="PK198_22290"
     CDS             2910..4547
                     /locus_tag="PK198_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HRF41547.1"
                     /translation="MNHCIKILLFCLALLSWQGLGAQLLDQRVSVQYADASISDVLSG
                     ISRNYGTRFAYSSDFVPVSRRVSVEAHNEPLRRVLDRVFEDQPVQYAAINGQVVLKVD
                     RNRQQEALGRIEKPKIRPVQTSPIYPQEVSKDEMAAIRRQRERWEHLADIERRKMENI
                     EGGVQAMPPPPVPVMNELPDDDPVRRLAQISLLPMIGTNALRSAQITNDLSLNILWGV
                     SGGVSGIEVGGFGNTVLGDVTGVQVAGVGSIVGGDVTGTQIGGLFNICEGRVTGVQAG
                     GVFNISGEAEAVQAAGLFNISRNNMSGVQAAGLFNIAHQGAKGLQAGTLFNISGGYTH
                     LQTALLFNSTRGDVRDGQYSLLYNRARQVEGIQFGLVNVADTVAGMPIGLFNFIKKGY
                     NRVEISASESLYGNIAVKFGAHAFYNIILGGARWESGNPEETAWGLGYGLGSVNKLGS
                     NVLINTELTAMHIHEGGQWTRPLNLLNRLHLSLDVRTKSNLSFFAGPTLNFMLSKRID
                     PDTGLRGSGLPPHTIWEGNAGKARAQAWIGFTAGIRV"
BASE COUNT         1106 a         1286 c         1343 g          880 t
ORIGIN      
        1 gatcatcatg ggcatagagg aagacgaccc caacgagacc ttcgaggacg tgatgaccaa
       61 gttggacatg ctcatcgggc tgcgcgaaat caaaacccaa gtgcgcaacc atgcccgcta
      121 catccagttt ttgcaactcc gcaaagaaaa aggctttgaa gaaaacgagg gcatcaatgt
      181 acactccgtt tttataggca atcccggcac cggcaaaacc accgtagccg gcatgatggg
      241 caagctgtac aaaaaaatgg gcctcctcac caaaggccat gtgcatgagg tggaccgtgt
      301 ggacctcgtg ggcgagtaca tcggccaaac ggcccccaaa gtacgcgagg ccatcgagcg
      361 ggccagaggc ggcgtgctgt tcattgacga agcctattca ttggcccgtt ccaacgacga
      421 cagcaaagac ttcgggcgcg aagtgattga aatcttagtg cgcgaactct ccaacggccc
      481 cggcgacctg gccgtcatcg tggcgggtta ccccaaagaa atgaaacatt tcctcgcctc
      541 caatccgggc ctgaaatcgc gtttcaacca tcattttgaa ttccgggact acctcccgca
      601 ggagctttcc gaaattgcgg aatttgcagc ccgcgaaaaa ggcatcgaac tgacgccgga
      661 agcccgcaaa aaaattgacg acgtcatcat tgatgcctac cgacgccgcg accgcacttt
      721 cggcaatgcc cgttttgtgt tcgatacgct cgaaaaagcc aaaatcaatc tgggactgcg
      781 cgtcatggcc atgcccgacc cccaactgtt ggagcaggaa cgcctggcca gggtggagct
      841 gtacgatgtg gccggtatcg gcaacacaga gcgccgcgat ctgcccaata ttcctgtgga
      901 cgagcagctt cttcaggaaa gcctcgacga gatgaaccgc ctcatcggca tggaaaaagt
      961 gaaagcgcag atcaaagagt tggtgtggtt ggtgcgctac taccgcgaaa ccggcaaaga
     1021 cgtgctcaac agtttctcgc tgcacactgt gttggtgggc aatcccggca ccggcaaaac
     1081 caccgtggcg cgcatcctca ccaaaatata caaagcgctg ggcatgctcg aacgcggcca
     1141 tgtggtagaa accgaccgcc agggtttggt ggcgggctat atgggccaaa ccgccatcaa
     1201 aaccgccgaa aaggtggaag aatctatggg cggcgtgctg ttcattgacg aagcctacgc
     1261 cctcacggcg cgcatcggcc ccaacggcga ctttggcgat gaagccatcc aaacgctgct
     1321 caagcgcatg gaggaccatc gggggcagtt ttttgtgttt gtggccggtt accccgacaa
     1381 catggagcaa ttcctcaagg ccaatccggg cttgaattcc cgcttcgaca aagtgctgaa
     1441 attcgaggat tacgaacccg aagacctcag ccggattgca cacatcatgc tggccgagca
     1501 aaacttagtg cccacgcccg aagcggaagc acacctcaag gattacctca attttgtgta
     1561 tgaataccgc gacaagtatt tcggcaatgc gcgtaccgtg cgcggcgtcg tgcaggaggc
     1621 tgtcaaaaac cagcatctga ggctcgccgc tttgcctgcc gaagaacgcg acgccacctc
     1681 catcaacatc ctcacgctgg aagatgtggc cggtttccgc accgataaaa gcgatgcggt
     1741 gttcacgaaa aaaaccattg gtttccgtcg ggcatcgggc gagggaaccg tgggcagcgc
     1801 ggcggggagc tgagtttttt ctcaaaaaaa actttctctc cgaatagggg tacccgcccc
     1861 ctccctgcat cctcccaaca gcaaagacga tatggcagat caaaatatga acgctgaaca
     1921 aatcggtaag tacctgagcg gtgaagcgtc gccggccgag gtagccgaat tgatggcctg
     1981 ggtagagcaa tctcaggcca ataaagagtt ctttgacaat atgttgcaac tgtgggccat
     2041 cccggagccg cagcctgcaa tgcctccatt gaacgcctcc aaagcctggg cagcgctgga
     2101 atcggccata gaccaaagag cgcccgcacc gaaaaatcgg ggcatcattt ccatgaaaat
     2161 gctgctgcgc gtggccgccg tcctgttgcc cctcgccctt gcgctgtggt ggacgctggg
     2221 ccgaaatgac gcgccgacag aggtgctgat ggcccaaacc atcgcgcagg agcgcaaaac
     2281 catcaccctt cccgacggaa gtacggtgca gttgaatgaa aactctgcat tgcactaccg
     2341 tcaggatgcc gatgcgcgct acgtcaccct gagcggcgaa gcgttctttg aagtcaaaca
     2401 ccttgatggc aagcctttta tcatcgaaag tggcgaggcg cgtactacgg tgctgggtac
     2461 ctccttcaat gtgcgggcct acccgaacga ggagcgcgtg gaagtgagcg tcagtaccgg
     2521 caaagtacag gtggaggcca ttgcggcgcc gcaacagaaa gcaatgttgg ccgccggcga
     2581 ggccgtagcc gttcagaaaa aagacctccg attggccgca gccgaggaaa ttgcgaacaa
     2641 tgcaacagcc tggaaaaacc gccaattccg atttgacgat acgccggtag atgaggtagt
     2701 tctcacgttg gaacggtatt tcggggtgga catagcgttg gagcgggcgg cgataggcgc
     2761 ttgccgattc agcggacact atgacaaccc gcaattggaa gaggttttgg aggctctgtc
     2821 cttcgccata gatgcgcaat gggaacagcg cgccgaagtg attagcctgt ccggcaacgg
     2881 ttgccgatga aacaacgcca aaaaaccaaa tgaatcattg tattaaaatc ctgttgtttt
     2941 gcttggcgct gttgtcgtgg cagggtctcg gcgcccaatt gctcgatcag cgcgtatcgg
     3001 tgcaatacgc cgacgcctcc atctccgacg tcttgtcagg catcagtcgc aactacggca
     3061 cccggtttgc atacagcagt gatttcgtgc cggtgtcgcg ccgcgtgagt gtggaagcgc
     3121 acaacgaacc gctgcgccgc gtattggatc gggtgtttga agatcagcct gtgcaatatg
     3181 ccgccatcaa cgggcaggta gtactgaagg tggaccgcaa ccggcaacag gaggctttag
     3241 ggcgcattga aaaacccaaa atccggcccg ttcagaccag cccgatttac ccgcaagagg
     3301 tcagcaagga tgaaatggcc gccatccgcc ggcagcgcga acgttgggag catctggccg
     3361 atatagagcg gcgcaagatg gaaaacattg agggcggcgt acaagccatg ccgcctccgc
     3421 ctgtgccggt gatgaacgaa ctacccgacg acgatccggt gcgccgtttg gcccaaatat
     3481 cgctgctgcc catgatcggc accaacgccc tgcgcagcgc gcaaatcacg aacgacctgt
     3541 cgctcaatat tttatggggc gtcagcgggg gcgtttccgg cattgaagtg ggcggtttcg
     3601 gcaataccgt gctcggcgat gtgaccggtg tacaggtggc cggcgtaggc agtattgtgg
     3661 gcggcgatgt gaccggcacg cagataggcg gcttgttcaa catctgcgaa gggcgcgtga
     3721 cgggcgtgca ggcaggaggc gttttcaata tttccggcga ggccgaagcg gtgcaggcgg
     3781 cggggctgtt caacatttcc cgaaacaaca tgagcggcgt acaggcggcc gggctgttca
     3841 atatagcaca tcagggcgcg aaaggactac aggccggcac gctcttcaac atcagcggcg
     3901 ggtacacgca tttgcaaacc gccctgcttt tcaactcgac gcgcggggat gtgcgcgacg
     3961 gacagtattc gctgttgtac aatcgcgccc gccaggtaga gggcatccaa ttcggccttg
     4021 tcaacgtagc cgacacagta gccgggatgc ccatcgggct gttcaatttt ataaaaaaag
     4081 gctacaaccg ggtggaaatt tccgcttccg aaagcctgta cggcaacatc gccgtcaagt
     4141 ttggagcgca tgccttttac aacatcattc tgggcggcgc tcgctgggaa tcgggcaatc
     4201 ccgaagaaac ggcctggggc ttaggctacg ggttggggtc ggtcaacaag ttgggttcca
     4261 atgttttgat caataccgaa cttacagcca tgcacatcca cgaaggcgga cagtggacgc
     4321 gcccgctcaa cctgctcaac cgtctgcacc tgagcttaga tgtgcgtacc aaaagcaacc
     4381 tcagcttttt tgcagggccg acgctcaatt tcatgctctc caaacgcatt gatcccgaca
     4441 ccggcctgcg cgggtccggc ctgcctccac acacaatatg ggagggcaat gcgggaaaag
     4501 cgcgggcaca agcctggatt ggatttactg cgggaattcg ggtgtgaagc ggttctcggt
     4561 gggcggtttt caggggtgag gggtgagtat gtgaggagta agcaacccgg aacct
//
LOCUS       DAORLF010000393         4613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10220_length_4613_cov_1.657745, whole genome shotgun sequence.
ACCESSION   DAORLF010000393 DAORLF010000000
VERSION     DAORLF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_4613_cov_1.657745"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    331..364
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            655..>1690
                     /gene="cas1"
                     /locus_tag="PK198_22295"
     CDS             655..>1690
                     /gene="cas1"
                     /locus_tag="PK198_22295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRF41548.1"
                     /translation="MEIHVSSFGARLRMRGEIFVLATPDLSGAGNDTTQEIAPHQVES
                     ILLYEKGGSISADAMALALRHQVDILLCDQLGMPLGRLWALDASPPLAIQTGQFRMMG
                     TPRSMDLVKEWLSRKFRRKLAFLERLERYREGEKAQLIQTTRHTLAEYYARLQQVSTQ
                     NIGQAAASFRGIEGSAARLYFGTLSALLQPEYQFDGRSRRPAADIFNALLNYGYGMLY
                     RTVEKALIEAGIHPYAGFHHGLAKRQKAMVFDVIEAYRPWVDHIVFVLCSRKTAGSRH
                     VKEQGEGLWLSNEGKTLMVKAFHEFFKKAELPFGNVELPPVKILKEEMRALAAYFATL
                     GREVESLLPNA"
     assembly_gap    1691..1716
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1828..>2025
                     /gene="cas2"
                     /locus_tag="PK198_22300"
     CDS             1828..>2025
                     /gene="cas2"
                     /locus_tag="PK198_22300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRF41549.1"
                     /translation="MYYTVCYDIGDAKTRRLAVKLCKKAGLVRMQRSVFTGRAPAALI
                     RQIEAELMPLLKDRNDSVAVQP"
     assembly_gap    2026..2074
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2166..>2361
                     /locus_tag="PK198_22305"
     CDS             2166..>2361
                     /locus_tag="PK198_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41550.1"
                     /translation="MYGNFHTEIDLTLAQLDQKAAKFKALKHPGDLAALLKTDAAALA
                     KLASKPHYQVFSIPKPGGDKR"
     assembly_gap    2362..2573
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2574..3171
                     /locus_tag="PK198_22310"
     CDS             <2574..3171
                     /locus_tag="PK198_22310"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRF41551.1"
                     /translation="HTVTSRHLQWIFGTLFEFPAPLCNQLIGLTSYKGVLPMGAPTSP
                     VLSNLAALPLDAGLQVLANKCDALYTRYVDDITFSFDAQPDVQFTQAARQVVESQGFT
                     LNEGKIRLSFRQDEPEVTGLILKSPKPDVSRGFIKSLKKDIKLYRYLCSPDMLQRGIV
                     NAGVLEKLQRSIRGQLAFLSFIRGKQDKTYRRLAALEG"
     gene            complement(3415..3813)
                     /locus_tag="PK198_22315"
     CDS             complement(3415..3813)
                     /locus_tag="PK198_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41552.1"
                     /translation="MLTLATARILVISPTDTDSWEMRIFISRMPFENVFERDYVVRQM
                     VPTDEYDFAIFDARKLPKIFENTVLNTEDESYLKLFRQYLKMPLRYIVFFGEFLHDLD
                     RERCPSANSKFTLYARIRELIEFINAYRER"
     gene            complement(3818..>4613)
                     /gene="cmr6"
                     /locus_tag="PK198_22320"
     CDS             complement(3818..>4613)
                     /gene="cmr6"
                     /locus_tag="PK198_22320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HRF41553.1"
                     /translation="RRFELDVPAESELEKVKKLAHQLDLSIFCGFSFEKEEKPARLPM
                     SKHDVFFDALPVNFNEKDQLLGRDALTPHGDNPLKNPIPLPFLKVMPGVVFGFYFRLQ
                     DSKIGSLTITAEHKRRIFGEILCTVGAGAKTNVGYGQFDTNNIADRIQPMNKAAASPK
                     TIGKFESQSPAISQPAAATSTKLKSFPFNKSLNGKNIIGEVQRIVGSEVWFKVLNVEG
                     FEKEISDKNPSASRFEIGKQYELRVNEVSSDKGILKAKISSLKPLN"
BASE COUNT         1052 a         1098 c         1109 g         1033 t
ORIGIN      
        1 gtttacgagt ttcttggtct cgttgccgtt gccttagtcg ttgtcggtcg caattctgcc
       61 ttagtcgaag attggtttac gagtggccgg gacacctatg tgttcactct accaaacgga
      121 ggtcgcaatt ctgccttagt cgaagattgg tttacgagac ccccctgctt ctcgggggga
      181 tggtttacga cggtcgcaat tctgccttag tcgaagattg gtttacgaag caactatcag
      241 gcacaatgcc cgcagttgca ggtctcaaag gtaaaaactc ccccgcagtt ttggggcgtt
      301 tttcgttaag aaaatcttaa attttttcta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnngccgaa aatcttacaa aactactgca tctccgcaga ggtaaaatat tgaaaaacaa
      421 atttatacaa caatgaacat ttcaggaaat gctcaaagag gcgttccgca gttttgcact
      481 ttttcaagca tggaagcagc aaaaccccga aatttgatag tgaaaagaaa caacaagttt
      541 ttaccaaatt tttccaacca tgacacctct cagtaatcag cacgaccgaa ggctcagaaa
      601 cacccgaaac cgcgacgtac ccattttgct ccaccgtttt aaatgcacgt tgttatggaa
      661 attcatgtat cttctttcgg cgcccggctc agaatgcggg gcgaaatctt tgtgcttgca
      721 acgcccgacc tgagcggggc aggcaacgac acgacgcagg aaattgcgcc gcatcaggta
      781 gagtccattc ttttgtacga aaaaggcggc tcgatcagcg ccgatgcaat ggctttggcg
      841 ctccgacatc aggttgatat cttgctgtgc gatcagctcg gcatgccatt gggcaggctg
      901 tgggcgctgg atgcttcgcc tccattggcc atacaaaccg ggcagttccg catgatgggt
      961 accccgcgca gcatggattt ggtgaaagaa tggctgagcc ggaagttccg gcgaaagctg
     1021 gccttcctgg aacgcctcga gcgctaccgg gaaggtgaaa aagcccagtt gattcaaacc
     1081 acccgccata cgctggcgga gtactatgcc cgactccaac aggtttccac acaaaacata
     1141 ggccaggccg cagccagctt tcgcggcatt gaaggcagcg cggcgcgctt gtacttcggc
     1201 acactcagtg cgctgctgca gcccgaatac cagtttgacg ggcgcagccg gcggccggca
     1261 gccgatattt tcaacgcact gctcaactac ggctacggca tgttgtaccg cactgtagaa
     1321 aaagcgctga tagaggccgg catccatccg tatgcaggct ttcatcacgg gctggccaaa
     1381 cgccagaagg cgatggtgtt tgacgtgata gaagcgtacc gcccctgggt ggaccatatc
     1441 gtctttgtgc tgtgttcgcg caagacggcc ggttcgcgcc acgtcaaaga gcagggagag
     1501 ggcctgtggt tgtccaatga aggcaaaacc ctgatggtca aagcatttca tgagtttttc
     1561 aaaaaggcgg aattgccttt cggcaacgtg gaactgccgc ctgtgaaaat cctcaaagaa
     1621 gaaatgcgcg ccttggccgc ctattttgcc acgctgggcc gggaagtgga gagcctgttg
     1681 ccgaacgctg nnnnnnnnnn nnnnnnnnnn nnnnnncgtg acaccgatgc tgcagtcggc
     1741 ctgcgccttc agaacgggaa cgggatacag ttgccgttag gacagatttg aaaacacatt
     1801 cttccactca cccaatcatt ttcaaccatg tactacaccg tttgctacga catcggcgac
     1861 gccaagacca gaaggctggc cgtcaaactc tgcaaaaaag caggcttggt gcgcatgcag
     1921 cgctccgtat tcaccggcag ggcgcccgcc gcactcattc gccagataga agccgagctg
     1981 atgccgctgc tcaaagaccg caacgacagc gtggcggtgc aacctnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgca caagaaaaac ctggcaggtt
     2101 cgcgcctcat cgtgtttgtg tagcagcgat ctgcgccttt ttcatcatcc caaaaacaaa
     2161 ctactatgta cggaaacttt cacacagaga ttgatctgac gctggcccag ctcgatcaga
     2221 aagcagccaa attcaaggca ttgaaacatc ccggcgatct ggcggctctg ctcaaaacag
     2281 acgcagccgc attggccaag ctcgcctcca agccgcatta tcaggtattc agcataccca
     2341 aacccggcgg cgacaaacgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcacacc
     2581 gtcaccagcc gtcatttgca gtggattttc ggcacgctgt ttgagtttcc ggcgccgctg
     2641 tgcaaccaac tcatcgggtt gacgagctac aaaggcgtac tgcccatggg cgctcctacg
     2701 tcgccggtat tgtccaatct ggcagcattg ccattggacg cgggcttgca ggtgctggcc
     2761 aacaagtgcg acgccctcta cacccgctat gtggacgata tcaccttcag tttcgacgcc
     2821 caacctgatg tgcaattcac gcaggcggcg cgtcaggtgg tagaatcgca gggctttacg
     2881 ctgaacgagg gcaaaatacg cctgagtttt cgtcaggacg agccggaagt gacgggcctc
     2941 attctcaaat cgcccaaacc ggacgtgagt cgcggcttca ttaaaagcct gaaaaaagac
     3001 atcaagctgt accgctacct ctgctcgccc gatatgctgc aacgcggcat tgtgaatgcc
     3061 ggcgtgctgg aaaaactgca aaggagcatc cgggggcagt tggcatttct gagcttcatc
     3121 cgaggcaaac aggacaagac gtaccgccgc ctggcagcgc tggaggggta ggcggcggag
     3181 agccggaagc tgccccggtt gttctcagca ttcaggatgc cgcctggcag cgctggaggg
     3241 gtaggcggcg gagagagaag atttttccga gatgtcaaag aacggggtga ggagcgaatg
     3301 tgtttgcagg ggtatctcac catttatccc aattttcaat gtaccctgtg taactgcttg
     3361 gcaacgttaa tcaatgcctg atccttgttc ggttgcgtat ccatgaatct atatttacct
     3421 ttcccgataa gcattgataa attcgatcaa ttcgcgaatg cgggcgtaga gtgtgaattt
     3481 ggagttggca gaagggcagc gttcgcggtc gagatcatgc aggaactctc caaagaatac
     3541 aatatagcgt aaaggcattt tgagatactg gcgaaagagt ttcaagtaag attcgtcttc
     3601 ggtattcagc acagtatttt caaatatttt cgggagttta cgcgcatcaa aaatggcaaa
     3661 atcatactca tctgtgggca ccatttgcct gactacataa tcgcgttcaa atacattttc
     3721 aaaaggcatt ctcgatatga aaatgcgcat ctcccagcta tcagtgtcgg taggggaaat
     3781 gacgaggatg cgagcggtgg cgagggttag catactttta gtttaaaggt ttaagcgatg
     3841 atatttttgc ttttagaatg cctttgtcgg agctgacctc atttacccga agttcgtatt
     3901 gcttacctat ctcaaatcgg cttgcggagg ggtttttatc gctgatttct ttttcaaagc
     3961 cttccacatt gagtaccttg aaccatactt ctgatccgac tattcgttga acttcaccta
     4021 tgatattttt cccgttcaaa gacttattga acgggaacga tttcaatttt gtcgatgttg
     4081 cagcggccgg ctgagatatg gcaggagatt ggctttcaaa tttccctatg gttttcggcg
     4141 aggcggcagc tttattcatt ggctgtatgc ggtcagcgat attattggta tcaaactgcc
     4201 catatcccac atttgtcttc gcccctgcgc ccactgtgca aagtatctcg ccaaaaatgc
     4261 gccgtttgtg ttcggcagtg atggtcaatg aaccgatttt tgagtcttgc agccgaaaat
     4321 agaagccaaa aaccactccg ggcatcactt tcagaaacgg cagtgggatg ggatttttca
     4381 aagggttgtc gccgtgtggg gtgagcgcat cacgtccgag tagttgatct ttttcattga
     4441 aatttacggg tagcgcatca aaaaacacat cgtgcttcga catcggcagg cgagcaggct
     4501 tttcttcctt ttcaaaactg aatccacaaa aaatggatag gtctaattgg tgcgccagtt
     4561 ttttcacctt ttccaattcc gactcagcgg gcacatccaa ctcgaagcgc cga
//
LOCUS       DAORLF010000394         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10269_length_4594_cov_2.054417, whole genome shotgun sequence.
ACCESSION   DAORLF010000394 DAORLF010000000
VERSION     DAORLF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10269_length_4594_cov_2.054417"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /gene="ligA"
                     /locus_tag="PK198_22325"
     CDS             <1..1103
                     /gene="ligA"
                     /locus_tag="PK198_22325"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRF41554.1"
                     /translation="RWAIAFKFKAKQATTKLLNVEFQVGKVGSITPVAKLDPVALAGV
                     TVSSVSLHNEDFIRNKDLRLGDTVLVERAGDVIPYIVKPMEELRDGSEQEIAFPTNCP
                     VCQSKLVRAEDEAAWRCENPDCEAQLLQRMIHHVSKNAMDIDGFGESQIDRFYRLGWL
                     HSIADIYRLDYEAIAQLEGFGEKSAGNLRKAIEKARQNPLHRLLHSLSIHHLGIKAGK
                     LLAAEIQNLWELKDWDEERFHQIKDIGPVLTHNVIRFFQNEKNVALLKDMESLGVNFQ
                     QTEDDRPKAVSADAPLAGKTILFTGTLTKMGRKEAQAKAEAAGAKNISAVSGNLDYLV
                     VGEDAGSKLKKAQALGTVTILTEDAFLELVNG"
     gene            1136..1756
                     /locus_tag="PK198_22330"
     CDS             1136..1756
                     /locus_tag="PK198_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF41555.1"
                     /translation="MPATTVLPDSAKTATESPVQAVKKISWEVFQRRYLHRENGFKYE
                     WVRGRVEKSQRSMDRTQLYILRNLLQHFMKLSQTGVVSGQLLAEADLFFQPDLHRRPD
                     VCWLTHAQINALARPDAREIPKFIIEIISSNDQINRVKKKLLEYREAGVQVVWHIYPA
                     YKQVEVFTGTRLENATLYEGEQVCSAAPALPGFEMAVSAVFELAEV"
     gene            complement(1768..>3054)
                     /locus_tag="PK198_22335"
     CDS             complement(1768..>3054)
                     /locus_tag="PK198_22335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41556.1"
                     /translation="AAGNGYQTDGPVTRFSYHGNPDNPAQWSMHGEGLTIGDPSCIGS
                     VLLGRLSPGQSATVETAYLYVREPGLNHLQNVAAMYDQLETLQAAYENGFADLCTVPE
                     LCIHDCVWPGDANADGIANHEDLLAIGRGLNQTGPERTGSLNWAPHFAQDWSVVGMQH
                     TDTNGNGAVEVNDAQITLLHYNQTKLGYLAPPVVYPPGPELSLNPIQPNAFEQIMEGQ
                     SILTRVSLLEVPELKGLAYSLEYDPRYFECIVNTATTFNDLNLVRPDNEKHQIDFVWY
                     RNQPGAFIGATNTLHLFRIKARDFFGEPLPSATSYIRFKNVIGIREDGTRIPMGGTDM
                     RADFIGIFVNTETPTPLADILVFPNPAEDLLQLRFPGQHLDGVTLLTAAGIPLRRLKG
                     TFFDALSLDLQGLPAGMYFLRMEQDGCTVVKKVVRR"
     assembly_gap    3055..3128
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3129..4148)
                     /locus_tag="PK198_22340"
     CDS             complement(<3129..4148)
                     /locus_tag="PK198_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41557.1"
                     /translation="MKKMLLMAFALPFATLLTAQCGQPHQATYLHGNEIRASVTSSGD
                     LFWASGDHGRFQIIGEPGRPATIFAQGLQLGGFDPAGNFRGASQTYRSTTNRSDFPAG
                     PIIEGQIPAGDYCSNWDRVWSVTSSEVEAHLSDFADNQQIDAPIASIMQWPGRGNPRF
                     EALMGFPLPNTHQGLAPFFDANSDGIYDPLAGDYPFVESVAHLPEQITWAVFNDQYRG
                     NAHSGLPSMQVEIQRTAWAMRCEGNSPLNRTVFVSYKVINRGTELLDSLVMGLWHDFD
                     LGCHLDDYMGSAPQHNAFFVYNAQPEDLTPCSGGIATFGPNPPVQAVAILNRPLHAFF
                     TRAGGA"
     gene            complement(4284..>4594)
                     /locus_tag="PK198_22345"
     CDS             complement(4284..>4594)
                     /locus_tag="PK198_22345"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF41558.1"
                     /translation="FPYIYDLGEAWLAHTGLPFVFAAWVSNSPLPEEFIEPFNAALQD
                     GLRQIPELMFLLPSSAPGFDLHAYFTENISYELDAPKRKAMGMFLQEIGASKAVLQGV
                     "
BASE COUNT         1099 a         1207 c         1232 g          982 t
ORIGIN      
        1 cgcgctgggc cattgccttc aaattcaagg ccaaacaagc caccaccaaa ctcctcaacg
       61 tagaatttca ggtgggcaaa gtgggtagca tcacgccggt agccaagctc gatcccgtag
      121 cgctggcagg cgtcaccgtc tcttccgtga gcctgcacaa cgaggatttt attcgcaaca
      181 aagacctccg actcggcgat acggtgctgg tagagcgggc cggcgacgta attccctaca
      241 tcgtgaagcc gatggaagaa ctgcgcgacg gctcggagca ggaaatcgcc tttccgacca
      301 actgcccggt gtgccaaagc aagctcgtgc gcgccgaaga cgaggctgcc tggcgctgcg
      361 aaaatcccga ctgcgaagcc caactcctcc aacgcatgat tcaccacgtt tccaaaaacg
      421 cgatggacat tgacggattc ggcgaaagcc agattgatcg tttctaccgc ctcggttggc
      481 tgcacagcat agccgacatc taccgccttg attatgaggc catcgcacaa ctcgaaggat
      541 tcggcgagaa gtcggcgggc aatctccgca aagccatcga aaaagcccgg caaaatccgc
      601 tgcaccgcct gctgcacagc ctcagcattc accacctcgg catcaaggca ggcaaactgc
      661 tggccgccga aatccaaaat ctgtgggaac tgaaagactg ggacgaggag cgctttcatc
      721 agatcaaaga catcgggccg gtgctgacgc ataatgtgat ccgctttttt caaaatgaaa
      781 aaaacgtggc gttgctgaag gacatggaat cactgggcgt caatttccaa caaaccgagg
      841 atgaccgtcc caaagccgtg agcgccgatg cgcccttagc cggtaagacg atcttgttta
      901 ccggcacgct caccaaaatg gggcgcaaag aggcgcaggc caaagcagaa gcagccgggg
      961 ccaaaaacat cagcgccgtg agcggcaacc tcgattattt ggtggtgggc gaagatgcag
     1021 gcagcaagct gaaaaaggcg caggccctcg gcacggtgac gattctgaca gaggatgcgt
     1081 ttttggagtt ggtgaatggg taaatttgac ttatttttgc aaaaacattt tacaaatgcc
     1141 ggctaccact gttctgcccg attctgcgaa gactgcgaca gaaagccctg ttcaggcagt
     1201 taaaaaaatc tcttgggagg tctttcagcg tcgttatctt catcgcgaaa acggatttaa
     1261 atacgagtgg gtgcgcggtc gggtggaaaa atcccaacgc tccatggatc gcacccaact
     1321 ctatattctc aggaatttgc tccagcattt tatgaaattg agccagactg gcgtcgtttc
     1381 cggtcagttg ctcgccgaag cagacctttt ctttcagccc gatcttcacc ggcggcccga
     1441 tgtttgctgg cttacacatg ctcaaatcaa tgccctggcc cggcccgatg ctcgtgaaat
     1501 accgaaattt atcattgaaa tcatttcttc caacgaccag atcaaccggg tgaaaaagaa
     1561 gttattggaa taccgggaag ccggagtaca agtcgtctgg cacatctacc cggcgtataa
     1621 acaggtggaa gtctttacag gaacccggct tgaaaacgcc actttatatg aaggcgagca
     1681 agtttgttcc gcagcgccgg ccttgccagg ttttgaaatg gcagtgagcg ctgtttttga
     1741 actggcggag gtttgatata agcccgccta cctccgcacc acttttttca ccactgtaca
     1801 cccgtcttgt tccatgcgca ggaagtacat tcccgccggc aacccctgca aatcgagcga
     1861 aagcgcatca aaaaacgtcc ccttcagcct ccgcaacggg ataccggccg cagtgagcag
     1921 ggtgacgccg tctaagtgtt ggcctggaaa tcgcagttgc aacaaatctt cggcgggatt
     1981 gggaaaaacc agaatatcgg ccagaggcgt cggggtttcg gtattcacaa aaatgccgat
     2041 gaagtcggcc ctcatgtctg ttccgcccat ggggatgcgg gttccgtctt cgcggatgcc
     2101 gatcacattt ttgaaacgga tataagaagt agcggagggg agcggctcgc cgaaaaaatc
     2161 gcgcgctttg atcctgaaca agtgcagtgt attggtcgca ccaataaagg cgccgggttg
     2221 gtttctgtac cacacaaagt caatttggtg cttctcgttg tcggggcgca caaggttcag
     2281 atcattaaat gtagtagccg tattgacgat gcattcaaaa tatcgggggt cgtattccag
     2341 agagtatgcc agccccttga gttcgggtac ctccaacaga ctgactcgcg tgaggatgct
     2401 ttgcccctcc ataatctgct cgaaagcatt aggctgaatc gggttgaggc tcaattcagg
     2461 tccgggagga tagaccaccg ggggcgcaag gtaccccaat ttagtctggt tgtaatggag
     2521 cagcgtaatc tgggcatcat tgacctcaac tgcgccgttg ccgttggtat ctgtatgttg
     2581 cataccgacg acactccagt cttgtgcaaa atgcggcgcc cagttgaggc tccccgttcg
     2641 ttcggggccg gtttgattca ggccccgccc gatggcgagc aaatcctcgt gattggcaat
     2701 gccgtctgca ttggcatcgc cgggccatac acagtcgtgt atgcacaatt cgggtactgt
     2761 gcaaagatcg gcaaagccat tttcataagc agcctgaagg gtttcaagct ggtcatacat
     2821 ggcggccacg ttttgcaggt ggttcaaacc cggctctctc acatagaggt aggcagtttc
     2881 tacggtggca gattgccccg gactcagtcg tccgagcagc acactgccga tgcaggaggg
     2941 gtcgcctatg gtcaaaccct cgccgtgcat agaccattgg gcagggttgt cggggttgcc
     3001 gtgataggaa aagcgcgtta ccggcccgtc ggtctgatat ccgtttccgg cagcnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnngg cgccgccggc ccgcgtaaag aaggcatgga gcggccggtt cagaatggca
     3181 acggcttgta ccggcggatt ggggccgaat gttgcgatgc cgcctgagca aggcgtcaaa
     3241 tcttcgggct gagcattata cacaaagaaa gcattgtgtt gaggggccga tcccatataa
     3301 tcgtcaaggt ggcaaccgag gtcaaaatca tgccacagcc ccattaccaa tgaatcgagc
     3361 aactccgtgc cgcgattgat gactttgtaa ctgacaaaga ccgttcgatt gagcggtgaa
     3421 ttgccttcgc atctcatcgc ccaagccgtc cgctgtattt ccacttgcat agatggaaga
     3481 ccagaatggg cattgccccg gtattggtcg ttgaaaactg cccaggtgat ctgctccggc
     3541 aaatgagcca cgctttctac aaagggataa tcgccggcca gagggtcata aatcccgtcg
     3601 ctgttcgcat caaaaaacgg ggccagcccc tgatgggtat tgggcagggg aaagcccatg
     3661 agtgcttcaa acctgggatt gccgcgcccg ggccattgca taatggaggc aatcggggcg
     3721 tctatttgct ggttgtcggc aaaatcggag aggtgtgcct ccacttcgga actggtcact
     3781 gaccagacgc gatcccagtt gctgcaatag tctccggcag gaatctgccc ctcaatgatg
     3841 gggccggcag gaaaatccga gcggttggtc gttgatctgt aggtttggct tgcgcccctg
     3901 aaattgccgg caggatcaaa accgcccaac tgcaggccct gtgcaaaaat agtggccggg
     3961 cgtccgggtt cgccgatgat ctgaaacctg ccgtggtcgc cgcttgccca gaacaagtcg
     4021 ccggaggaag taacggaggc tcttatttca tttccatgca aataagtggc ttgatgaggc
     4081 tggccgcact gggccgtcaa cagggtggca aatggcaagg caaatgccat gaggagcatt
     4141 tttttcatgg ttgcagtttt ttttgaaatt cactgcaaga gtataaaaaa aatccacgca
     4201 agtcaacaca agcccttact tattcggcct tttgtctttt taatgacatg attcacagat
     4261 acttaataca aggggcttga tcgctacact ccttgcaaca ccgccttcga cgccccgatt
     4321 tcctgcaaaa acatacccat tgccttgcgt ttgggcgcat ccaattcgta gctgatgttc
     4381 tcggtgaaat atgcgtgcaa gtcaaaccct ggagccgacg agggcagcag aaacatcaat
     4441 tcgggtatct ggcgcaagcc atcctgcaat gcagcgttga acggctctat gaactcctcc
     4501 ggcagcgggc tgttgctcac ccaggctgca aaaacgaaag gcaaaccggt atgcgccagc
     4561 catgcctcgc ccagatcata aatgtacgga aaac
//
LOCUS       DAORLF010000395         4593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10271_length_4593_cov_2.049361, whole genome shotgun sequence.
ACCESSION   DAORLF010000395 DAORLF010000000
VERSION     DAORLF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10271_length_4593_cov_2.049361"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2252)
                     /locus_tag="PK198_22350"
     CDS             complement(<1..>2252)
                     /locus_tag="PK198_22350"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF41559.1"
                     /translation="AEPFLVQAKDTRREVFGEQHPAYSWSLNNLAQFYLATAAYDKAE
                     PLLREAIEIKARTLGNEHPDYAKSLLNLARLFARKGQWMQADSLLHKGLDIYAAKLSG
                     QHADYLAGLHQQAVLYINVGKSSEAEQVLLQEKAAREAAGDTLSSQYALCWQALGDVE
                     QGRKDWAKAEHGYTKATSLLNEHFGKAHPAQAVLSRQQALLYRAAGQYERAVQYALAA
                     ADVEQRFLLRAVRYMSERDLAAFAESLSETRPLLYDLAASEAAGFCYDDALFIKGFLL
                     NAASEIRRAVGADSLAAKKMEELRVMYRRLAAEYARPASERKNVGDWENQAEVLEKEL
                     KRTVGGYAQAIQKTIWQEVKQALRPGEAAIEFIHYKVPGAEDSVMYAALVLRFDLPEP
                     RRVSLFEEKQLAALMKNAKGVNKAEWIQNMYASGRGTSLYDLIWQPLEPALEGVSTIS
                     YSVSGLLHRIQLDAVVVSPLLRGEERAETLCDRYRLIRLNSTRTLVRTAASPVIGGKA
                     LIYGGIEYEADTSAQATAPVAARSALPDSTLRGHDWGYLKWTEVETSAVADILAEGGV
                     AVTLRQGREADEATLKHAAAPDILHIATHGFFFPDSELVKSSPDEVVSGFKSSPQPLM
                     RSGLILAGGNYAWKHGRPLQPGEEDGILTAYEISRMDLGGTRLAVLSACETGLGELRG
                     YEGVYGLQRAFQLAGARYLLMSLWQVPDFQTQELMTAFYANMVDHHKSIPDAFEAARQ
                     ALRKKYPNPF"
     assembly_gap    2253..2368
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2369..3009)
                     /locus_tag="PK198_22355"
     CDS             complement(<2369..3009)
                     /locus_tag="PK198_22355"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41560.1"
                     /translation="MRKLLCIACLLMPLLSAAQWRQQLDSLSQAAMGRAGQRAFDEAL
                     EINARALRLARDSGGLLSEVYGDASLDCGLIFLQKGDYPQAELHFLESEKAYMATLGP
                     EHRLRILAADNLSDVYLRMRNFEKSEYYHLMVREIREKILGRNHKDYISALGNLANLY
                     TEMGEFERAEPLYVETLLLREQLNGKDHRDYATALNNRGLNFLHSGRYEQAEPL"
     gene            complement(3019..>3766)
                     /locus_tag="PK198_22360"
     CDS             complement(3019..>3766)
                     /locus_tag="PK198_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41561.1"
                     /translation="LQQWRIERSVAAAAQLRKGYERRARRQRRVWSGLAAACVLGALA
                     WWYFPKSTNSALPPSPPVELPADSIAPQPPAPPSPSSGPVIAERQASPGSFSPAPNVR
                     GSENEAAASQALLDQLWYGAYPPASWQPDEELAPADTLLRQRSFARAYVRLQRLEMRR
                     PDNDTLRLMKGYCLLEMREGAEALRYLENLEARQPEWKAYLQWHRGLARLLNGEQAAA
                     VQEFRVIAAQAGHPFAAQARKALGVLEKRK"
     assembly_gap    3767..3790
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3864..4472)
                     /locus_tag="PK198_22365"
     CDS             complement(3864..4472)
                     /locus_tag="PK198_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41562.1"
                     /translation="MSRPKWFSDASPCRRFADDEQLVQALRQWDNEAIACLQWNCHSA
                     VKSIVRRCGLPDERTEEILNSATMVFLQKTAEGAYQYQGQSPGAYLVEIARRLSLAEA
                     RKQGRNPADLDSVPEPADADAAWLNRRQEAAETVQFLLDRLGSPCAEVIRLHHIEGYA
                     DEEVIRLGLTPYSTIASLKVKRSDCMKKLVQSALQWKNSTNI"
BASE COUNT          910 a         1354 c         1209 g          980 t
ORIGIN      
        1 aaaacggatt cggatatttt tttcgcaggg cctgccgggc cgcctcgaag gcatcgggaa
       61 tagacttatg atggtccacc atattggcat aaaaagccgt catcagttcc tgcgtctgaa
      121 aatcaggcac ttgccaaagc gacatgagca aataccgcgc cccggccaat tgaaaagccc
      181 gctgcaatcc ataaacgccc tcataaccgc gcaattctcc cagccccgtt tcgcaggccg
      241 acagcaccgc caatcgggtg ccgccgagat ccatccggct gatctcataa gcggtgagga
      301 tgccgtcttc ctcgccgggt tgcagagggc ggccatgctt ccaggcatag ttgccgccgg
      361 ccaaaatgag gccggaacgc atcagcggct gcggactgct tttgaagccc gatacaactt
      421 cgtcgggaga tgacttaacc aactcagaat caggaaaaaa gaagccatgt gtagcgatgt
      481 gcaaaatgtc gggcgctgcg gcatgtttca gcgtggcctc gtcggcttcg cgcccctgac
      541 gcaaggtgac ggctaccccg ccctcggcca gaatgtccgc caccgcggag gtttccactt
      601 ccgtccattt cagatagccc cagtcatggc cgcgcagggt agaatcgggc agtgcgcttc
      661 gggccgcgac gggcgcagtt gcctgagccg atgtatctgc ttcatactca atacctccat
      721 aaatcaaggc ctttccacca atgaccggcg acgcagcagt gcgcaccagg gttcgggtgc
      781 tgttgagccg gatcagccgg tagcggtcgc acagggtttc ggcacgttct tctcctctca
      841 acaaaggtga cacgacgacg gcgtccaatt gaatgcgatg cagcagcccc gacaccgaat
      901 aactgatggt agaaacgccc tccaatgctg gttccagcgg ctgccaaatc agatcgtaaa
      961 gacttgtacc tctgccagat gcgtacatat tttgaatcca ttcggctttg ttgacgcctt
     1021 tggcattttt catcagcgcg gccaattgtt tttcttcaaa aagcgatacc cggcgcggtt
     1081 cgggcagatc gaagcgcaac accaacgcgg catacatcac gctgtcttct gctccgggca
     1141 ctttgtaatg tataaattca atggccgcct cgccggggcg caatgcctgt ttcacctcct
     1201 gccagatcgt tttttgaatg gcctgcgcgt agccgccaac ggtgcgtttc agttcttttt
     1261 ccaaaacctc tgcctgattt tcccaatccc ccacattttt tcgctccgac gccgggcgcg
     1321 catactcggc agccaaacgt cggtacatca cgcgcagctc ctccattttt ttagcggcca
     1381 aagaatcggc ccccaccgcg cggcgaattt ccgaggcggc attcagcaaa aaacctttga
     1441 taaacagggc gtcgtcgtaa caaaaaccag ccgcctccga ggccgccaga tcgtacaaca
     1501 atgggcgcgt ttcgctcagc gattccgcga aagcggccaa atcccgctcc gacatgtatc
     1561 tcacggcccg caacaggaaa cgctgctcca catctgccgc cgccaaagcg tattgcacgg
     1621 cacgctcgta ttgcccggca gcacgataca aaagcgcttg ctgacggctc aaaacggcct
     1681 gcgcgggatg cgcctttcca aaatgctcgt tcaacagcga ggtggctttt gtataaccat
     1741 gttcggcttt cgcccaatcc ttccggccct gctctacatc gcccagcgct tgccaacaaa
     1801 gggcatactg cgacgacagg gtatctccgg cggcttcacg ggcggccttc tcctgaagta
     1861 aaacctgctc cgcctcggat gattttccca cgttgatata caaaacagcc tgttggtgca
     1921 gccccgcgag gtagtcggcg tgttgcccgc tcaatttggc tgcatagatg tccaggccct
     1981 tgtgcagtag cgaatcggcc tgcatccact ggccttttcg ggcgaagagc cgggccaggt
     2041 tgagcagact tttggcatag tcggggtgct cgttccccag tgtccgggct ttgatttcga
     2101 tggcttcccg caacagcggt tccgccttgt cgtaggcggc tgtggcaagg taaaactgtg
     2161 cgaggttgtt gaggctccag gaataggccg ggtgctgttc gccgaaaact tcccggcgag
     2221 tgtctttggc ttgaacgaga aaaggctcgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cggttcggcc tgttcatacc tgccggaatg
     2401 caggaaattc aggccccggt tgttgagcgc ggtggcatag tcgcggtggt cttttccgtt
     2461 cagttgttcc cggaggagca gggtttcgac gtagaggggt tcggcgcgtt caaactcgcc
     2521 catttcagta tagagattgg ccaagttgcc gagcgcggag atgtagtctt tgtgatttct
     2581 gcccagtatt ttctctcgta tctctctgac catcaagtgg taatactccg atttttcaaa
     2641 attccgcatc ctgagatata cgtcggagag gttgtcggcg gccaaaatgc ggaggcgatg
     2701 ctccggcccc aaggtcgcca tgtatgcttt ttcactttcc aaaaaatgaa gctctgcctg
     2761 tggataatcg cctttttgca aaaaaatcag cccgcaatcc aggctcgcat ctccgtacac
     2821 ctccgacagc aagccgcccg aatcgcgcgc cagccgcaaa gcccttgcgt tgatttccag
     2881 cgcctcgtcg aaagcccgct gccccgccct gcccatagcc gcctgagaga ggctgtccaa
     2941 ttgttgccgc cactgcgccg ccgataacag cggcatgagc agacacgcga tgcaaagcag
     3001 ttttctcatc ttataaatct atttcctttt ctccaacact ccaagcgcct ttcgggcctg
     3061 agccgcaaac ggatgcccgg cctgcgcggc aatgacgcga aattcctgca cggcagcagc
     3121 ctgctctccg ttcagcaacc gcgccaaccc ccggtgccat tgcaaatacg ctttccactc
     3181 cggttgccgg gcctccagat tttccaaata ccggagcgcc tcggcccctt cacgcatttc
     3241 cagcaggcag tagcctttca tcaggcgcag ggtatcgttg tcggggcgac gcatttccaa
     3301 acgctgtagc cgcacataag cccgtgcaaa gctcctctgg cgcagcaggg tatcggcagg
     3361 cgccaactcc tcatcgggct gccaacttgc cggcgggtaa gcaccgtacc acaactggtc
     3421 gagcagggct tgcgaagctg ccgcctcgtt ctcacttcca cgcacattgg gcgccggtga
     3481 gaacgaaccg ggactggcct gcctttcggc gatgacgggg ccggaggagg gcgacggcgg
     3541 tgcaggcggc tgcggagcga tgctgtctgc cggcaactct acggggggac tgggcggtag
     3601 cgccgaattt gttgatttgg gaaaatacca ccaagccagc gctcccaaca cacaggcggc
     3661 agccaatccg ctccataccc ggcgctgccg gcgagcgcgg cgttcgtacc ctttgcggag
     3721 ttgcgcggca gcggctactg accgctcaat gcgccattgt tgcagcnnnn nnnnnnnnnn
     3781 nnnnnnnnnn gctgcctgta aatcaggctg ttcggccaat cttttttcaa aagccgggcg
     3841 ctcttcgtcg ctcatttggc cgctcagata tttgtcgagt tcttccattg cagggctgat
     3901 tgtacaagtt ttttcatgca gtcgctgcgt ttcactttca acgacgcgat ggtggaataa
     3961 ggcgtaaggc ccagccgaat cacttcctca tcggcgtagc cctcaatatg atgcagccga
     4021 atcacctcgg cgcagggcga gcctaaacgg tcgagcaaaa actgcacggt ttcggcggct
     4081 tcctgtcggc gattcagcca tgccgcatcg gcgtcggcgg gttcgggcac gctgtccaaa
     4141 tcggcaggat tacgcccctg ttttcgggcc tcggccagcg acaacctgcg ggcaatttcg
     4201 acgagataag cccccggcga ttggccctga tattggtacg ccccctcggc tgttttttgc
     4261 aaaaaaacca tagtggccga attgaggatt tcctccgtcc tttcatcggg cagcccgcag
     4321 cgccgtacaa tgcttttcac cgcgctgtgg cagttccatt gcagacaggc gatggcctcg
     4381 ttgtcccatt ggcgcaaagc ctgcacgagc tgctcgtcgt cagcgaaacg ccggcagggc
     4441 gaagcatccg aaaaccattt tggtcggctc atcgtcggct gtacaggtga atgtcgtttg
     4501 aatgcacaaa agtaagtgca agtgatgtat ttatcttttt tatggcagcc gcccactcct
     4561 gcaaagagct gttagcttta tctccgcaat ggc
//
LOCUS       DAORLF010000396         4583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10304_length_4583_cov_1.986307, whole genome shotgun sequence.
ACCESSION   DAORLF010000396 DAORLF010000000
VERSION     DAORLF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10304_length_4583_cov_1.986307"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK198_22370"
     CDS             complement(<1..636)
                     /locus_tag="PK198_22370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRF41563.1"
                     /translation="MTPDFRKKLIDWGAEVERPLPWKGIRDPYKIWLSEILLQQTRAA
                     QGLPYYLKFVERFPTVTDLASAEDDEVMKLWEGLGYYARARNLLKAARLVAFEMNGRF
                     PDTYEGLRQLPGVGPYTAAAIASFAYDAPHAVLDGNVFRVLSRFTGLAEPVDTTAGRK
                     RFEELAQSTLDAARPAAYNQAIMDFGATICKPRSPLCGQCVQSKECEAFRHG"
     gene            complement(640..1830)
                     /locus_tag="PK198_22375"
     CDS             complement(640..1830)
                     /locus_tag="PK198_22375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41564.1"
                     /translation="MNTRLIFSALLCNVFLLFGHAQTYLPGQTYYGLNQYIEYRPGTL
                     PIIITAPHGGYLQPSEIPDRNCTDCVTVMDAFTEELSREIADAIAQRMGCRPHLIINR
                     LHRRKLDANRDLAIAANGNPLAETAWFDFHRFIQAAKDSSAVHFGRGLLLDMHGHGHS
                     VQRLELGYLLYGSELRMSDAVLNTTEFINYSSIRGLVQNNLQNQTHAQLLRGASALGT
                     IYENQFFRAVPSLPDPFPLVGQDYFSGGYNTERHGSIEGGPIDAIQIECNFSGVRDTP
                     AHRSAFAQATAEALEQYLQTHYFGASFPDNACSGATSVQETNVAGIRLHPNPATHQIY
                     MQWTGNEPIMAVLYDARGRRFSATISQNAEELKLEVSHLVAGWYVLQIQSGQETAMLR
                     FLKI"
     gene            complement(2036..>2339)
                     /locus_tag="PK198_22380"
     CDS             complement(2036..>2339)
                     /locus_tag="PK198_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF41565.1"
                     /translation="DTDPFGVIRAWFSGSNSIDLLIDAPDKDYRKALDGVAGLRKLVE
                     KELAVTGDEAYPFMELALHGLAEFNVVNKDMVEAGVSFRDLLAGMLNDTDFDEDEY"
     assembly_gap    2340..2518
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2519..>3151)
                     /locus_tag="PK198_22385"
     CDS             complement(<2519..>3151)
                     /locus_tag="PK198_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF41566.1"
                     /translation="HRDQRYVEKLATPDVSVADLIGDVDPIKAATLKLPYSDERVIHF
                     GLVPHSHRSIFVINELPDLQARIQVSLFNILQEGDIQIRGFKLRLPLDIQFVFTANPE
                     DYTNRGSIITPLKDRIESQILTHYPKTIEIARKITRQEALLAADQKAHITVPDLLEIL
                     LERIAFTARESELVDSKSGVSARLSISAYENLVSTAERRMLINKEQKATAR"
     assembly_gap    3152..3173
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3174..3525)
                     /locus_tag="PK198_22390"
     CDS             complement(<3174..3525)
                     /locus_tag="PK198_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF41567.1"
                     /translation="MQPNIQTLGQLKASGYQPKSIKEELRDNLVEILRQRKKVFEGII
                     GFDDTVLPDIERAVLSRHNILLLGLRGQAKTRIARLLVHLLDEYIPVVAGSELNDDPL
                     HPISRRAKDIIAEHR"
     gene            3620..>4583
                     /locus_tag="PK198_22395"
     CDS             3620..>4583
                     /locus_tag="PK198_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41568.1"
                     /translation="MLFRFPLLRCSVWLFTFLPALLTAQADKGMNLLSKGNPVAAEAA
                     FRADLDNPKEAPLAMYGLSLVYADAKFERKNIDTAFVFIETCDKTYRKMPPDLKSKIV
                     KKLSSAMIADQRKAVIDKAFARAKEQATVEAYEFFLNNFPKPLTKLKRAALTERNALA
                     LQRAKATGQHNEIQALLDRYGSSLATDTPNLYAEAQRALFQFFINEKGWGAFAEFKTA
                     YPENMVLKDTLLTVFQQAVQTPAPGALELFVDKYPGSVFLPLATDSLAARLALSGSMA
                     SCKKFLGKYPQHPQSQSIWKRLYQQVTTGATNPEILERFLNDHPA"
BASE COUNT         1029 a         1160 c         1168 g         1025 t
ORIGIN      
        1 cccgtgacgg aaggcttcgc attccttcga ctgaacgcat tgtccgcata gggggctacg
       61 cggtttacaa atagtagccc cgaaatccat gatggcctga ttgtaggctg cgggccgggc
      121 tgcgtccaag gtcgattgcg ccagttcctc aaaacgcttt cttccggcag tggtatccac
      181 cggttcggct aagcctgtga aacggctcag cacccggaat acattgccgt ccaacaccgc
      241 atggggggca tcgtaagcaa aagaagcaat ggccgccgcc gtgtaaggac ctactccggg
      301 caactgcctc aagccttcat aagtatctgg aaatctgccg ttcatctcga aagcgaccaa
      361 gcgggcggct ttcagcaggt tgcgggcgcg agcgtagtag cccagccctt cccacagctt
      421 catcacctcg tcgtcttcgg ctgaggccaa atcggtgacg gtgggaaatc gctccacaaa
      481 tttgagataa tacggcagtc cctgcgcggc gcgggtttgc tgcaaaagta tttccgacaa
      541 ccagattttg taaggatcgc ggatgccttt ccagggcagc gggcgttcga cttcggcgcc
      601 ccagtcaatt agctttttgc gaaaatcagg ggtcatgttt cagattttga gaaagcgcag
      661 catggccgtt tcctgcccgc tctggatttg taaaacatac cagcctgcaa ccaaatgcga
      721 gacctctaat ttcagttcct ctgcattctg gctgatcgtg gcggaaaaac gacgcccccg
      781 cgcatcgtac agaacagcca taatcggttc attacccgtc cattgcatat aaatctgatg
      841 tgtagccgga ttgggatgca gtcgaatgcc cgccacgttg gtttcctgta cagaggtcgc
      901 gccggaacag gcattatcag gaaaagaggc cccgaaatag tgcgtttgca ggtattgttc
      961 caaagcctct gcggtggctt gcgcaaaggc gctgcgatgg gcgggcgtgt cccgcacgcc
     1021 gctgaaattg cactcaattt ggatggcatc tatcggcccg ccctcgatgg agccgtgccg
     1081 ttcggtgttg taacctcccg aaaagtaatc ttgcccgacc agaggaaagg gatcgggcag
     1141 actgggtact gcacgaaaaa attgattttc gtagatcgtt ccgagggcgg aggcgcctct
     1201 caacagttga gcatgcgttt gattttgcag gttgttttgt accaaacccc gaatgctgga
     1261 gtaattgata aactcagtgg tattaagaac tgcgtcgctc atgcgcaact cactgccata
     1321 caggagataa ccgagttcca accgctgcac gctgtggccg tgaccgtgca tatccaacag
     1381 cagcccgcga ccaaaatgca ctgcgctgga gtctttggcg gcttgtataa aacggtgaaa
     1441 atcgaaccag gcagtttcgg ccaatgggtt gccgttggcg gcgatggcaa gatcgcgatt
     1501 ggcatccaac tttcttctgt gtaagcgatt gatgatgaga tgcggtcggc aacccatacg
     1561 ctgggcaatg gcgtcggcta tttcgcgcga aagttcttcg gtgaacgcat ccatcaccgt
     1621 cacgcagtcg gtgcagttgc ggtcgggtat ttccgaaggt tgcagatagc cgccatgcgg
     1681 ggccgtaatg atgatgggca aagtacccgg cctgtactca atgtactgat tgagaccgta
     1741 gtaggtttgg ccgggaaggt aggtttgcgc atggccgaat aacaaaaaca cattacacag
     1801 caaagcactg aaaatcaatc gagtattcat tcattataac catttagcag cttccccaaa
     1861 ccacgaactt cgagctacga gtttaagtat cataaaaatt acaaattact cattttcaat
     1921 agtgaatttg aaatttcccc ataaggaaaa tctaatttcc cgacgaaaat gcaagctcat
     1981 agctcgtggc ttgcagcttg cagcttttgg gggtatagta aacagatacc acgattcaat
     2041 actcatcttc atcaaaatca gtatcgttca acatgccagc cagcaggtct cggaacgata
     2101 cgccggcctc caccatgtct ttgttcacca cgttgaactc cgccagcccg tgcagcgcca
     2161 attccataaa cgggtacgcc tcatcgccgg tcaccgccaa ttctttttct accaacttgc
     2221 gcaatccagc cactccgtcc aatgcttttc ggtagtcttt gtcgggtgca tctatcaata
     2281 gatcaatgga attactacct gaaaaccagg cgcgtatgac gccgaacggg tcggtatccn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     2521 ggcagtcgct ttttgttctt tattgatgag catacggcgc tctgcggtgc tcaccaagtt
     2581 ttcatacgcc gagatactca ggcgcgccga caccccgctt tttgaatcca ccaactcgct
     2641 ctcgcgggcg gtgaaagcga tgcgctccaa tagtatttcc aacaaatccg gcaccgtgat
     2701 atgtgccttt tgatcggcag ccaagagggc ttcctgccgg gtgatcttgc gagctatctc
     2761 tatggttttg ggatagtggg tcagaatctg gctttcgatg cggtccttca gcggggtgat
     2821 gatgctgccc cggttggtgt agtcttccgg gttggccgta aaaacgaact gaatatcgag
     2881 cggcaggcgc aatttgaagc cgcgaatctg aatgtcgcct tcctgcaaaa tattgaacaa
     2941 ggagacctga atacgggcct gcaagtcggg caactcattg ataacgaata tgctgcgatg
     3001 cgaatgaggc actaagccga aatggatgac gcgctcgtcg gagtagggca gcttgagggt
     3061 agcggctttg atggggtcca cgtcgccgat gaggtcggcc acgcttacgt cgggggtggc
     3121 caatttttcc acatagcgtt ggtcgcggtg cnnnnnnnnn nnnnnnnnnn nnncgcggtg
     3181 ttcggcaatg atgtctttgg cgcgccggga aatggggtgc agcgggtcgt cgttgagttc
     3241 gctgccggcc accacgggga tgtactcgtc gagcaggtga acgagcaggc gcgcgatgcg
     3301 ggttttggcc tgaccgcgca aacccagcag gagaatgttg tggcgagaaa gcacggcgcg
     3361 ctctatgtcg ggcagcacgg tgtcgtcgaa accgatgatg ccctcgaaca cttttttgcg
     3421 ttggcgcagg atttcgacga ggttgtcacg gagttcttct ttgatggatt tgggttggta
     3481 gcccgaagcc ttgagttggc cgagggtttg aatatttggc tgcataagaa tggtgttcgg
     3541 atgaaaaaaa acaaaaacgc aaaatgaagg gaatggttgc cggcattttg tgtaagtttg
     3601 gccattaaaa tctactccta tgctcttcag atttcccctg ctccggtgtt ccgtttggtt
     3661 gttcaccttc ttacccgccc tcttaacggc tcaggccgac aaaggcatga acctcctctc
     3721 caaaggcaat ccggttgctg ccgaggctgc ttttcgtgcc gatttggaca atcccaaaga
     3781 ggcgcccttg gccatgtacg ggctgtcttt ggtgtatgca gatgcaaaat tcgagcgcaa
     3841 aaacatagac acagccttcg tttttattga aacctgcgat aaaacgtacc ggaaaatgcc
     3901 gcccgatttg aaatcgaaaa ttgtaaaaaa gttgagcagc gccatgatcg ccgatcagcg
     3961 caaagccgtc attgacaagg cattcgcacg cgccaaagag caggctacgg tagaggctta
     4021 tgagtttttc ctgaacaact ttcccaaacc gctcaccaaa ctgaaacgcg ccgccctcac
     4081 agagcgcaac gcgctggccc tgcaacgcgc caaagctacc gggcaacaca acgagataca
     4141 ggccctgctc gaccgctacg gcagcagctt ggccaccgac acgcccaacc tgtacgccga
     4201 agcacaacgc gcactttttc agttctttat caatgaaaaa ggctggggcg ctttcgcgga
     4261 attcaaaacg gcctatcctg agaacatggt gctcaaagat acgctgctga ccgttttcca
     4321 acaggccgtt caaacgccgg ccccgggtgc attggagttg tttgtggaca aataccccgg
     4381 ctccgttttt ttgcctttgg ctaccgacag tctggcggct cgtttggcgc tgtctggcag
     4441 tatggcttct tgcaaaaaat ttctcggcaa atacccgcaa catcctcaat cacagagcat
     4501 ttggaagaga ttgtaccaac aggttacaac cggagctacc aatccggaga tcctcgaacg
     4561 cttcctcaac gaccatcccg cct
//
LOCUS       DAORLF010000397         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10323_length_4575_cov_2.137168, whole genome shotgun sequence.
ACCESSION   DAORLF010000397 DAORLF010000000
VERSION     DAORLF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10323_length_4575_cov_2.137168"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..424)
                     /locus_tag="PK198_22400"
     CDS             complement(62..424)
                     /locus_tag="PK198_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41569.1"
                     /translation="MNAVLNLGKYLFALPFAVFGAFHFMGLDSMASWAPGGKVGVIVS
                     GVALIAAAVSIIIGKLDKLASVLLAVMMLLFVALIHAKGASSGDQAATGNLLKDLMLA
                     GAALMYAKTMAKDNAVIG"
     gene            complement(605..1819)
                     /locus_tag="PK198_22405"
     CDS             complement(605..1819)
                     /locus_tag="PK198_22405"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HRF41570.1"
                     /translation="MTELAPIEKVCIQLLLKAPFYGHFLMGIPKSFDEHVETACVSLY
                     RRNMIQLKINPAFWAALSEDHKLGLIQHEVLHIALRHLNRVTAFTHRRLFNIAADLVV
                     NQYIEPPLLPPDSILPEQLLPWEPILGYRIEKEQGIDYYYAILLDILQKIPLPAPGNG
                     MAKDAAEGLSSWLQDDNKAMRRHVLWDEITRLDSSEERILEQFVRNASRQALDRTRHK
                     PSGRLPAALREMVEHIHLEPPRVNWRRTLRLFAASGTSTWLKSTIRKPSKRYGTVPGI
                     KLNRHHRLAIVLDTSGSIRTDEMRLFFSEVWQIWRRGAQITIIECDAKIQNVYPYAGS
                     TPDAVSGRGGTRFDEPLRWANEHLPDALIYFTDGHAPPPQTALRRPVLWVITPQGKED
                     VSHLPGRVLRMV"
     gene            complement(1816..2880)
                     /gene="ccsA"
                     /locus_tag="PK198_22410"
     CDS             complement(1816..2880)
                     /gene="ccsA"
                     /locus_tag="PK198_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF41571.1"
                     /translation="MKWIQQHGWKALGVLLLLYAFTAGLLVPLKPGILTASPQLLRAG
                     SEVTLRIRGYNSFYQEGQAGLRAWLKLDKERALAASSIQAVSETELDAVFQIPAVLPI
                     AAKTTPMTLILDNVVDGPSVLPDAVSVTQEAVDSLAGAAAWLNSPVQKLHPGPTGIRF
                     PFRNILYETIRNTYFHVALWFAMLLLFIGSVRHSARYLRRQDAEDDRRAAAYTRVGVM
                     FGILGLVTGAIWAKNTWNAYWSWDVKQNMTAIALFIYIAYFILRAAFEDAERKARLSA
                     VYNVFAFVALIPLIFVIPRLTDSLHPGAGGNPALGGEDLDNTMRMIFYPAIIGWTLLG
                     AWMAQLNYRIDRLADLHDDV"
     assembly_gap    2991..3074
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3208..>3833)
                     /locus_tag="PK198_22415"
     CDS             complement(3208..>3833)
                     /locus_tag="PK198_22415"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF41572.1"
                     /translation="RLAEWKGRGLPAYGLRADVTAAADRAAMIAAVEQRWGRLDVLVN
                     NVGMNIRKPFADYEEAEYRSIFETNLFSVLAITRLCFPLLQRSRGASVVNISSVAASV
                     DVRSGAPYGMTKAAELQLTRSLAVEWAPLGIRVNAVSPWYTRTPLAEPVLRQPERLEG
                     ILRRTPLGRIAEMEEPAAAAAFLAMDKSSYITGQNIVVDGGMMAGAL"
     assembly_gap    3834..3855
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4233..4336
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1047 a         1149 c         1224 g          945 t
ORIGIN      
        1 acgacaaagg gcgagaggaa tcactccgcc cgcccttcgc gcattcttga attaaaaaaa
       61 attagccgat cacagcgttg tccttcgcca tggtcttggc atacatcagt gcagcgccgg
      121 ccagcatgag gtctttcagc aaattgcccg ttgcagcctg gtctccggaa gatgcgccct
      181 tggcatgaat gagcgctaca aagagcaaca tcatcactgc gagcagcaca gacgccagct
      241 tgtctaattt accaataatt atgctcacgg cagcagcgat aagcgccacg ccggaaacga
      301 tgacgccgac tttaccgccc ggcgcccagg aggccatgct gtcaagtccc ataaaatgga
      361 atgccccaaa aacggcaaag gggagggcaa acaagtactt gcctaagttg agaactgcat
      421 tcatagttgg tcaattttta aaagtgatga aaataggtgt agatacaatc gttctgacaa
      481 agataatatc agtttgaata aacaagcatt tttttcaatt ttttttgaat aaatgaggga
      541 ttcaagaatt ttgtattaca aaaaagtcaa atcgcaagga gcggtatttt ttcaaaggtc
      601 atgcctatac catccgcagc acccgcccgg gcaaatgcga tacatcttct tttccctgcg
      661 gcgtgatgac ccacagcaca ggtcttctca aggccgtttg gggcggcggc gcatgaccgt
      721 cggtgaaata gatgagcgca tcgggcaggt gttcgttggc ccaccggagc ggctcgtcga
      781 aacgggtgcc tcccctaccg ctgacggcgt cgggggtgct gcctgcgtag ggatatacat
      841 tctgaatttt ggcgtcacat tcgatgatgg tgatctgtgc cccgcgccgc catatttgcc
      901 atacttcgct gaaaaacagg cgcatttcat ctgtacgaat gcttcccgac gtgtcgagta
      961 caatggcgag ccgatgatgg cggttcagct tgataccggg caccgtgccg taccgtttgg
     1021 agggttttcg gatggtactt ttcaaccagg tactcgtgcc ggacgccgca aacaggcgca
     1081 atgtccggcg ccagttcacg cggggcggtt ccagatgtat gtgttccacc atttcccgga
     1141 gtgctgcggg gagcctgccg cttggcttgt gccgggtgcg gtctaaggcc tgccgggaag
     1201 cattgcggac aaactgctcc aaaatgcgtt cttcgctgct gtccaatcgg gtgatttcat
     1261 cccagaggac atgtcgtcgc atcgctttgt tgtcgtcctg aagccaggat gacagccctt
     1321 ccgcagcatc tttggccatg ccgttgccgg gagccgggag cgggattttt tgcaggatgt
     1381 ccaacaggat agcgtagtaa taatctatgc cctgctcttt ttcaatgcgg tagcctaaga
     1441 tgggttccca gggcagcagt tgttcgggca gaatgctgtc cggtggcaat aacggcggtt
     1501 caatgtattg attcacaaca agatcggcgg caatattaaa aagccttcgg tgtgtaaatg
     1561 ctgtgacgcg gttgagatgc cgcagtgcga tgtggagcac ctcgtgttgt atcaacccca
     1621 gtttgtggtc ttccgacaaa gcggcccaaa aggcgggatt gatcttcaac tgaatcatgt
     1681 tgcgccggta gagacttacg caagccgttt ctacatgctc atcaaacgat ttgggaatac
     1741 ccatgagaaa atgcccgtaa aacggagcct tcagcaggag ctgaatacac actttttcga
     1801 tgggagcaag ctcagtcata catcgtcgtg gaggtcggct aagcggtcta tgcggtagtt
     1861 gagttgtgcc atccacgcgc ccagcagcgt ccagccgatg atggccggat aaaaaatcat
     1921 gcgcatggta ttgtccaagt cttcgccgcc caatgccggg ttgccgcccg cgccggggtg
     1981 cagactgtcg gtgaggcgcg gaatgacaaa gatgagcgga atgagcgcca caaaagcgaa
     2041 cacattatac accgctgaaa gacgggcttt gcgctcggcg tcttcaaaag cggcgcgcaa
     2101 gatgaaataa gcgatgtaaa tgaacagcgc aatggcggtc atattttgtt tcacatccca
     2161 gctccagtag gcgttccagg tgtttttggc ccaaatagcg cccgtgacca atcccagaat
     2221 gccgaacata acgcccaccc gcgtgtatgc agccgcgcgg cggtcgtctt ccgcatcctg
     2281 acggcgcaga tagcgggcgc tgtgccgcac cgagccgata aaaagcagga gcatggcaaa
     2341 ccaaagcgcc acatgaaaat aggtattgcg gatggtttcg taaaggatgt ttctgaacgg
     2401 gaagcgaatg ccggtggggc cgggatgcag tttttgaacg ggcgaattaa gccaagctgc
     2461 cgcgccggca agggaatcca ccgcctcctg cgtgacggaa acggcgtcgg gcaacacaga
     2521 agggccgtca actacattgt cgagaatgag ggtcatcggg gtggttttgg cagcaatggg
     2581 cagcaccgcc gggatttgga aaacggcgtc caattccgtt tcggataccg cctgaatgga
     2641 acttgccgcc aaggcccgct ccttgtccaa cttcagccag gcccgcagac cggcttgccc
     2701 ttcctgataa aaactattgt aaccccgaat gcgcagcgtc acctccgaac cggcgcgcag
     2761 cagttgcggg cttgcggtga gaatgcccgg cttgagcggc accaaaagcc ccgctgtaaa
     2821 ggcatacagc agcaataaaa cgcccagcgc tttccacccg tgttgttgaa tccatttcat
     2881 ttgagaacaa gtgttgtcgt tgataaaaag tatttgtaac tattcagcat atcccctaaa
     2941 gctacaagct gcaagccacg accacgccct gcgtggttgt ttttttgttc nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnncgtgac tcgaaccacg cctgtggcgt ggtcgcagct ttttgcaggg
     3121 cgactgaata gtcacttcaa gaatacattt agaaatccca cacacacccg gcgaaggtac
     3181 gaataacccg atgattcctt caaaatctca caaagcgccc gccatcatac caccatccac
     3241 cacaatgttt tgtcccgtga tgtaagacga tttgtccatt gccagaaaag cggcggcggc
     3301 ggcgggttcc tccatttcgg caatacggcc cagcggcgtg cggcgcagga tgccttcgag
     3361 gcgttcgggt tggcgcagca cgggttcggc cagcggcgtg cgcgtgtacc agggcgaaac
     3421 cgcgttgacc cgaatgccca gcggcgccca ttctacggcg aggctgcggg tgagttggag
     3481 ttcggcggct ttggtcatgc cgtagggcgc gccggaacgc acatccaccg aggcggccac
     3541 cgaactgatg ttgaccacgg aagcgccccg actgcgttgc aacaaaggaa aacacaaccg
     3601 cgtaatggcc agcacgctga acaggttggt ttcaaaaata ctgcggtatt cggcttcttc
     3661 gtaatcggca aaaggcttgc ggatgttcat gcccacgttg ttcaccagca catctaagcg
     3721 gccccagcgc tgctccacgg cggcgatcat ggcggcccgg tcggccgctg ccgtcacgtc
     3781 ggcacgaaga ccgtaagcag gcaagccgcg ccccttccac tcggccaatc gcgnnnnnnn
     3841 nnnnnnnnnn nnnnnaacac ctcggctccc agcaataaaa actcttcggc gatggcccag
     3901 ccgattcctt tggtgccgcc cgtcacaagg gcgcatttgc cttccagcgt ccaactgctc
     3961 atttgatctc aggttttggc gcgaagataa ggctttggag ggaatctccg acgcggatgc
     4021 cgccggataa gcgcgccgga aggcagcggc gtacatggcg cgcgggcggg aattgatttt
     4081 acagcgtttt tcggcacctg gggttgactt tacaaaagga atggtttaac tttgcacacg
     4141 tcaaaagtgc tttgaatccc tgaagtgacc ggctaagctc cgaaaggctt tgctcaaaca
     4201 aggaagctgc cccgtcgcgc attcggattg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnngtat ggatgagttg tgcctaatta taaaatgaga cagtgagccg
     4381 acaagaagaa aatagacttg ttgcagcgac aaaattaggg gaaaagtacg agatttgggg
     4441 ctaaaaatat agccatgaat tggaaagagc caaaaaacga aagagaatgg agagccgtaa
     4501 ttgggtttga taatcagcgg tttaatcaat ggttcgtgaa gaatcgccgc tttgcggcgt
     4561 gggcttgtga gaaat
//
LOCUS       DAORLF010000398         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10325_length_4575_cov_1.899115, whole genome shotgun sequence.
ACCESSION   DAORLF010000398 DAORLF010000000
VERSION     DAORLF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_4575_cov_1.899115"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1390
                     /locus_tag="PK198_22420"
     CDS             128..>1390
                     /locus_tag="PK198_22420"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HRF41573.1"
                     /translation="MDEKFQVNAANGTASLSLPLPFSPGRNGFMPAISLGYNSGAGNG
                     IFGLGWDLSFPSILRKTDKRLPRYRDTEESDIFMFSGVEDLVPFLTVDDGRWTVDEKT
                     VSGYFIRRYRPRIEGGFSKIERITHPTHGMWWKVTTRDNIATFFGKSPEYRIADPADE
                     NRVFQWLPELSFDDKGNCVLYEFKEEDGAGYGDALYDKNRFGDDGMLKFTNRYLKRVY
                     YCPNAPFLPFKPYLLEGQPVPVDVRYYTPNPSGSDDFLMELVLDYGEHGDLPTNAQGT
                     TSVNYAEINPWPIRHDAFSTYRSGFEIRTARLGRRVLMFHHFEELGDEPCLVRSLDMQ
                     YRDWDLLSEPEEGKKMEATYLVYAIQRSYVRENDNSYRFKSLPPLEFEYQELCWSRVI
                     HSVAPEDAANAPVGLSSGYQWVDLYNEGI"
     assembly_gap    1391..1511
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1512..>4575
                     /locus_tag="PK198_22425"
     CDS             <1512..>4575
                     /locus_tag="PK198_22425"
                     /inference="COORDINATES: protein motif:HMM:NF023676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HRF41574.1"
                     /translation="QIQDLAADGRKQVVVNSRGLNGYFELTDENNWESFQAFAQIPNL
                     NLRDPNTRLLDLNGDGQPEIVVTEENVFTWYASAGTLGYDAPELAPKPYDEEQGPAIV
                     FADSTQSIFLADMSGDGLTDIVRIRNGNVCYWPNLGYGRFGAKVSMDQSPWFDHPDQF
                     NPAYLQLADISGTGATDIIYLGKNQFLAWLNLSGNAWSKPCDISPFPDTAQPNAVTVT
                     DLLGNGTSCIVWSSPLPAHAREPLRYIDLMSGQKPHILKSYRNNFGKKVEWTYKSSTH
                     YYLEDKKAGKPWITKLPFPVQVVSKVTVTDQWRKSTFANTYSYHHGYYDHTEREFRGF
                     GRVEQVDVEDFGKFVEGNIDSPYITDDKTLYQPPVKTVTWFHTGAFLGRERILSQFRE
                     EYFQPKIEDFTELELPEPAIEAENLTADEWREALRACKGMTLRQEVYELDVDALQQGE
                     QRPVKLFSTAFHNCWIRCLQPKGTNQHAVFLVGESEAITYQYELDLRASASESEGDPK
                     VLDPRIAHTLNLKFDAYGQPLQSVAVAYPRIATYETDDKLITEKAKALIREIQGETHI
                     AYTENRYTDGIDGEGYRLPIPCEVLTCELTGFKSGEKSPYFSLKDFLYFRLSKIHQPV
                     PPDGAPDPFLAVIDSGYHERPDPSKEKEQKRLVEHVRILYFSEDLQTPLSLRDSGSLA
                     LPYETYKLALTNDLLSAILGEKLPSLQAADEVYGDMLKRVLGEGGYRQFDDEPGLWWV
                     RSGIAGFAKDASDHFYLPECYTDPFGNVTTLTFDKYDLYIRESTDPAKNTVSVTNFDF
                     RVLAPLEMQDINENRSEVYFDTLGMPAAVAVKGKGTEGDNLEGVCSEISDADLIDFFM
                     GEYDPDKARAFLGNATARHLYYFGDESHPACAAGLVREKHVAQLAEGEISPLQAAFEY
                     SDGMGAVLVKKIQAEPENSAEPLRWIATGKTVLNNKGKAVKQYEPYFSTDENEAPNHR
                     FEEPHEQGVTPILYYDAPGRQVRVEMPDGTLSRVEFSPWFTRAFDANDT"
BASE COUNT         1152 a         1162 c         1135 g         1005 t
ORIGIN      
        1 ttcttcagga agaactgaaa ggtccggaga aacatccaat gcccgttcac aagagcagtc
       61 ctctaagtct attgccatcg acattccgca aatctctctc cccaaaggcg gtggcgccat
      121 caaaggcatt gacgagaagt ttcaagtaaa tgctgccaat gggacagctt ccctcagttt
      181 gcctctacct ttttcaccag ggcgcaatgg attcatgccg gcgatctcat tgggttacaa
      241 ctctggcgcc ggcaacggca ttttcggact cggctgggac ttgagtttcc cttccattct
      301 gcgaaaaacg gataagcgcc tgcctcgtta ccgcgacacc gaggaaagcg acatctttat
      361 gttttcgggg gtagaggatt tggtgccgtt tttaacggtg gacgatggac ggtggacggt
      421 ggacgaaaaa actgttagcg gctacttcat cagacgttac cgcccgcgta tcgaaggcgg
      481 attttccaaa attgaaagaa taacgcatcc aacccacggc atgtggtgga aagtgaccac
      541 ccgtgataac atcgccacgt tttttggcaa aagtcccgaa taccgcatcg cagaccctgc
      601 tgatgaaaat cgcgtttttc aatggctgcc cgaattgagt ttcgacgaca agggcaattg
      661 cgtcttgtat gaatttaagg aagaagacgg cgcagggtat ggcgatgcac tatacgataa
      721 aaatcgtttc ggggacgatg gcatgttgaa atttacaaac cgatacctca aacgcgtcta
      781 ttactgcccc aatgctccgt ttttgccgtt caaaccatat ttgcttgagg gacaacccgt
      841 acccgttgac gtccgatatt acacgcctaa cccaagcgga agcgacgatt ttttaatgga
      901 attggtgctg gattacggcg aacacggcga tttgcctact aatgcacagg gaacgacctc
      961 cgtgaactac gcagaaatca atccctggcc cattcgccac gatgcttttt ctacctatcg
     1021 cagcggtttt gaaattcgta ctgcgcgact tggccgtcga gtgttgatgt ttcaccactt
     1081 tgaggagctg ggagatgaac cctgcttagt gcgttcgctg gatatgcaat accgcgattg
     1141 ggatttgtta tcagaaccgg aagaaggtaa aaaaatggag gcaacctatc tggtctacgc
     1201 catccaacgc agttacgtca gggaaaacga caacagttat cggttcaaaa gcctgcctcc
     1261 ccttgaattt gaatatcagg aactgtgctg gagcagggta atccacagcg tcgccccgga
     1321 agatgcagcc aacgccccgg ttggactcag ttctggctac caatgggtgg acctctacaa
     1381 cgaaggtatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn ntgcaaatac aagacctcgc agcggatggc cgtaaacaag tggtggttaa
     1561 tagtcggggc ttaaacggct attttgagtt gacggatgaa aacaactggg aatccttcca
     1621 agcctttgca caaatcccca acctcaatct tcgcgatccc aatacccgcc tgctcgacct
     1681 caatggcgac ggacaaccgg agattgttgt caccgaagag aacgtcttta cctggtatgc
     1741 ttccgccggc acgctggggt atgatgcgcc cgaactggcg cccaaaccct acgacgagga
     1801 acaaggcccg gccattgtgt ttgccgacag cacccagagc atttttttgg ccgatatgag
     1861 cggcgatgga ctcaccgata ttgtgcgcat ccgcaacgga aacgtgtgct actggcccaa
     1921 cctcggttac ggccgctttg gggcaaaggt aagcatggat cagtcgccct ggttcgacca
     1981 ccccgatcag ttcaaccccg cctacctgca attggccgac atcagcggaa ctggagccac
     2041 tgacatcatt tatttgggta agaatcagtt tctcgcctgg ctcaacctga gcggcaatgc
     2101 ctggagcaag ccttgcgaca ttagtccgtt ccccgatact gcccagccca atgccgtgac
     2161 ggtgaccgac ctgctgggca acggcacttc ctgcatcgtg tggtcctcgc ctctgcccgc
     2221 ccatgcgcgt gagccgctgc gctacattga tctgatgagc ggtcaaaaac cgcatatcct
     2281 gaagagttac cgcaacaatt tcggcaaaaa agtggagtgg acctacaaaa gctccacaca
     2341 ctattacctc gaagacaaaa aagccggtaa accctggatc accaaattgc ccttcccggt
     2401 gcaggtggtg agcaaagtga cggtgaccga ccagtggcgt aaatccacct ttgccaatac
     2461 ctatagttac caccacggct actacgacca caccgagcgc gagtttcgcg gtttcgggcg
     2521 ggtggaacag gtagacgtag aggattttgg gaaatttgtg gagggcaata ttgacagtcc
     2581 ttacatcacc gacgataaaa cactgtatca accgccagtg aaaaccgtca cctggttcca
     2641 caccggcgcc tttctgggac gcgagcgcat tttatcgcag ttccgggagg agtatttcca
     2701 gccgaaaatc gaagatttta cggaactgga actgcccgaa ccggccattg aagccgaaaa
     2761 cctcactgcc gacgaatggc gcgaagccct gcgtgcctgc aaaggcatga ccctgcgaca
     2821 ggaagtgtac gagttggatg tggatgccct gcagcaaggt gagcagcgcc cggtgaagct
     2881 cttttccacg gcttttcaca attgctggat tcgttgcctg cagcccaaag ggacaaacca
     2941 acacgcggtg tttttggtgg gcgaaagcga ggcgatcact taccaatacg aattggattt
     3001 gcgcgcttct gcaagcgaat cagagggcga ccccaaggtt ttagaccctc gtattgcaca
     3061 taccctgaat ttgaagtttg atgcttatgg tcagcctttg cagtcggtgg cggtggcgta
     3121 tccaaggata gccacatatg aaacagatga taaactgatc acagaaaaag ccaaagcgct
     3181 gatccgggaa atacaaggag aaacgcatat tgcctatact gaaaatcgct acactgatgg
     3241 aatagatgga gaaggctatc gcttgcccat accttgtgag gtgttgacgt gtgaattgac
     3301 gggattcaag tccggcgaaa agtcacctta ctttagcttg aaagatttcc tgtatttccg
     3361 attgagcaaa atacatcagc ctgtacctcc tgatggagcg cctgatccat ttctggctgt
     3421 gatagattcg ggctaccacg aacgccctga tccatcgaaa gagaaagaac aaaaacgtct
     3481 cgttgagcac gtccgtatct tgtatttttc agaagactta caaactccac tttcacttcg
     3541 tgattcaggc tcattagcct taccttacga aacctacaaa ttggccctga ccaatgatct
     3601 gctgagcgcc attctgggcg aaaagttgcc ttcattacaa gcagcagatg aagtttatgg
     3661 cgacatgctc aaacgggttc tgggcgaagg cggctaccgc caatttgatg acgaaccggg
     3721 actttggtgg gttcgctcgg gtattgccgg ttttgccaag gatgcttccg accattttta
     3781 cctgcccgag tgctacaccg atcctttcgg caatgtgacc acactgactt ttgataagta
     3841 cgatttgtac attagagaaa gtactgaccc ggcaaaaaat accgtatcgg tcacaaactt
     3901 cgatttccgg gtgctggcgc ccttagaaat gcaggacatc aacgaaaaca ggagcgaagt
     3961 ttatttcgat acgctgggca tgcccgcagc ggtagccgtg aagggaaaag gcacagaagg
     4021 cgataatctg gaaggtgttt gttcagaaat aagcgatgcc gacctgattg attttttcat
     4081 gggcgaatac gatccggata aagcccgggc ttttctgggc aacgccacag cccgtcacct
     4141 ctattatttt ggcgatgaaa gccaccccgc ctgtgccgcc ggattggtac gggaaaaaca
     4201 cgtagcgcaa ctggcagagg gagaaattag ccccttgcaa gccgcctttg aatacagcga
     4261 cggcatgggc gccgtactgg tgaaaaaaat acaggccgaa ccggaaaata gcgctgaacc
     4321 actgcgatgg attgccactg gcaaaaccgt tttaaacaac aagggcaaag ccgtcaagca
     4381 atacgaaccc tacttcagta ctgatgagaa cgaggcgccg aatcatcgct ttgaagagcc
     4441 acatgaacaa ggcgtaacgc ccatccttta ctacgacgct cccggccgcc aggtacgggt
     4501 agaaatgccc gacggcaccc tcagccgggt ggagttttca ccctggttta cccgtgcttt
     4561 tgatgccaat gatac
//
LOCUS       DAORLF010000399         4569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10342_length_4569_cov_2.034559, whole genome shotgun sequence.
ACCESSION   DAORLF010000399 DAORLF010000000
VERSION     DAORLF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_4569_cov_2.034559"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1341
                     /locus_tag="PK198_22430"
     CDS             22..1341
                     /locus_tag="PK198_22430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF41575.1"
                     /translation="MMPYRFLLLFFVLMSAAYLPAQTPLTLEKAIELAMQRDWDLKNA
                     RLNTVLTENALQKVQAQRTPVVTGSGDLRYNPILQTVVIPGAAFGQPGGEDQKVRFGT
                     NFSLLLGVDASYKLIDPTYLTRTQLELMRASGNKLTERVVEQDLKSEVAAAYYELALQ
                     SAQEVLAAQRLSRARDLLAVMKTRAEAGAALPVDVQKSELDLLNAEALRVQSRNQKDR
                     SRLQLASLSGIQPEDILLPDNILNISSKELELAVVNVSEVNERPELLEINQRININAL
                     ELKLADQSYRPSLDAFANASMQHLSNDLALWQRWFPFAFVGLRANLPIYDGKIRQRNK
                     EGIGLQMQIQQNNMAALREELTLEMQSAAIELRNAATTLQYAESALNTSQSILQTDQT
                     RFREGALLFSELRNTEFALREAEAAYLSAAHQWLTVQLRRLRAGGKL"
     gene            complement(1355..>2413)
                     /locus_tag="PK198_22435"
     CDS             complement(1355..>2413)
                     /locus_tag="PK198_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF41576.1"
                     /translation="VDLRCLALGNSIGTGPQGLTAEVIEVRSLNEVDSLGRKGVEGKI
                     VFYNRPMDPRLTNTFAAYGGAVDQRGAGAARAGKYGAVGILVRSMTNRIDTIPHTGAM
                     SYAPDVKPIPAIAISTADAELLSSILKEETARVYMRSTSRMLPEQITHNVIGEIRGSE
                     FPDEIILVGGHLDSWDVAGGAHDDGAGCVHAMDVLRVLQRVGYQPKRTLRCVLFANEE
                     NGLVGGRTYAKVSNEKGEFHLAAIESDRGGFTPRGFTVDAEETVFADKFKQISAWLPL
                     LEAYGLYIRKGGSGADIGPLKSQKGVLIGFEPDSQRYFDFHHTPIDNMDGVNKRELEL
                     GTATIASLVYLLDKYGIK"
     assembly_gap    2414..2544
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2545..2754)
                     /locus_tag="PK198_22440"
     CDS             complement(<2545..2754)
                     /locus_tag="PK198_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HRF41577.1"
                     /translation="MKKLLFIGALALLWGGNALAQKSETDTDALFIKKIHDQALSDGR
                     CYEWLSDLCRVAPGRLAGSPAAAAAV"
     gene            2804..3364
                     /locus_tag="PK198_22445"
     CDS             2804..3364
                     /locus_tag="PK198_22445"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF41578.1"
                     /translation="MMPQLFHIDTVLLSQHTVIRRFRENDGEAFYELLDNNRSRIEEL
                     LALSASDIGSKEDCEAFVRRKLSNWLLQQSYAFGVWHTDGAKPIGYVELFKIDWSVPK
                     SCIVFFVDKEYESKGIMTEVIREIGRYAFSQLNMERIELFTASDNFPAQRLARKCGFR
                     REGDLRAFFRRPSGELVDVMLLAVTR"
     gene            3373..3735
                     /locus_tag="PK198_22450"
     CDS             3373..3735
                     /locus_tag="PK198_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRF41579.1"
                     /translation="MAQHNDTGQAGEELAAQFLEQQGFQILHRNWRFGRAEVDIVAMD
                     GPALVLVEVKARSSDHFGRPEDFVSAKKQRFLAEAASRYMEMYGHDWEIRFDVVAVLF
                     NPETEITHFRDAFFPEWG"
     gene            3792..4559
                     /gene="mazG"
                     /locus_tag="PK198_22455"
     CDS             3792..4559
                     /gene="mazG"
                     /locus_tag="PK198_22455"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRF41580.1"
                     /translation="MQDKLEAFGRLLTIMDTLREQCPWDRKQTLQSLRNLTIEETYEL
                     ADAILDDNLNDIKEEIGDILLHMVFYAKIADEKGAFHIGDAIHAVCDKLIARHPHIYG
                     DVQVKDEEEVKKNWEQLKLREGKKSVLQGVPRSLPAMIKAYRMQEKTKQVGFEWENSE
                     QVWEKVEEEISEFREAMQTGMSQEKREEEFGDVIFSLINYARFQGIDPETALERVNNK
                     FKKRFEYIEAHAPTDLRNMTLAEMDALWNEAKTAFNS"
BASE COUNT         1093 a         1224 c         1153 g          968 t
ORIGIN      
        1 ttatcagaga aaaattaaaa catgatgcca tatcgttttc tgctgttgtt ttttgtgctg
       61 atgagtgctg cgtacttgcc tgctcaaact cctttgacgc tggaaaaagc catcgagtta
      121 gccatgcagc gcgactggga cctcaaaaat gcccgcctca acacagtgtt gacggagaat
      181 gccctgcaaa aggtgcaggc acaacgcacg ccagtggtga ccggaagcgg cgaccttcgg
      241 tacaacccga ttttgcaaac cgtcgtcatc cccggcgcgg cattcggaca gcctggcggc
      301 gaggatcaaa aagtgcgctt cggcaccaat ttcagtctgt tgctgggcgt ggatgcatcc
      361 tacaagctca ttgatcccac ttatctcact cgtactcagt tggaactgat gcgcgcatcg
      421 ggcaacaaac tgaccgaacg cgttgtggaa caagacctga aatcagaggt agccgcggct
      481 tattatgaac tggcattgca atcggcccag gaggtcctgg cagcccaacg cctgtctcgt
      541 gcccgcgacc tgctggccgt gatgaaaacg cgcgcggagg ccggcgccgc attgcctgta
      601 gatgtgcaaa aaagcgaatt agacctgctc aatgccgagg ctttaagggt acaaagccgg
      661 aaccaaaagg accgcagcag gctacaattg gcaagcctgt cgggaataca gccggaagat
      721 attttactgc ctgacaatat cctgaatatc agttcaaaag aattggaatt agctgttgta
      781 aacgtctccg aggtgaatga gcgaccagag ttgttggaaa tcaaccaacg catcaacatc
      841 aatgctttgg agttgaaatt ggccgaccaa agctaccgcc cgtctttgga tgcctttgcc
      901 aacgcgtcca tgcaacacct cagcaacgat ttggccctgt ggcaacgctg gttccccttt
      961 gcttttgtgg gattgcgggc caatttgcct atttacgacg gcaaaatccg tcagcgcaac
     1021 aaagagggca tcgggttgca aatgcaaata cagcaaaaca acatggccgc cctgcgcgag
     1081 gaactgacat tggaaatgca aagtgccgcc atagaacttc ggaatgccgc cacgacgctc
     1141 caatatgcag aaagtgcgtt gaacacatcg cagtctattt tgcaaaccga tcagacgcga
     1201 ttccgcgagg gcgcactatt attcagcgag ttgcgcaaca cggaatttgc actccgggag
     1261 gcagaagccg cttatctcag cgctgcgcat cagtggttga cagtgcagct acgccggctg
     1321 cgagccggcg ggaagttgta ggtacatccc cgtcttattt gattccgtac ttatccaaca
     1381 ggtacaccaa agaagcgatg gtggccgtgc ccaattcaag ctcccgcttg ttcacaccgt
     1441 ccatattgtc aatgggcgtg tggtgaaaat cgaaatagcg ctgtgaatcg ggttcaaaac
     1501 cgatgagtac tcctttttgg ctcttcagcg gcccgatgtc ggcgcccgaa cctcccttac
     1561 gaatatacaa accgtaggct tccagcaagg gcagccacgc gctgatttgt ttgaatttgt
     1621 cggcaaatac cgtttcctcg gcgtccaccg taaacccgcg aggcgtgaac cctcccctat
     1681 ccgactcaat ggccgccagg tgaaattccc ccttttcgtt agatacctta gcgtaggttc
     1741 tgccgcccac caagccgttt tcttcattgg caaaaagcac gcatctaagg gttcttttag
     1801 gctgataacc aactctttgc aagacccgaa gtacatccat tgcgtgtacg cagcccgcgc
     1861 cgtcgtcgtg tgcgccgcct gccacatccc aggaatcgag gtggccgccc accaagatga
     1921 tctcatccgg aaattcgctg ccccggattt caccgattac gttgtgggtg atctgttcgg
     1981 gcagcatccg gctggtggag cgcatataca cccgggccgt ttcttctttc aaaatgctgc
     2041 tcagtagttc cgcatccgcc gtgctgatgg caatggccgg aatgggcttc acatcgggcg
     2101 cataactcat ggcgccggtg tgaggaatgg tgtctatccg attggtcata gagcgcacca
     2161 agatgcccac cgcgccatac ttaccggccc gcgccgcgcc tgcgccccgc tgatctacgg
     2221 cgccgccata ggctgcaaaa gtgttggtca gacgcgggtc catcgggcga ttgtaaaaaa
     2281 cgattttgcc ctccacgccc ttgcgcccca ggctgtccac ctcgttgagg ctgcgcactt
     2341 cgatcacttc ggcggtcagc ccctgcggcc cggtgccgat ggagttgccc agagccagac
     2401 aacgcaaatc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnntactgc cgccgcagcc gccggagaac ctgccagacg
     2581 accgggagca acgcggcaca gatcactgag ccattcgtaa cagcgcccgt cggacagggc
     2641 ctgatcgtgt atctttttga taaataaggc atctgtgtcg gtttcgctct tttgagccaa
     2701 cgcattacca ccccagagga gggccaaagc tccgatgaac aaaagttttt tcattttaaa
     2761 agtctttatg tcgtaccggg cgaagatagt atttttgcgc actatgatgc cgcaactctt
     2821 ccatattgac accgttttgc tcagccaaca cacggtcatc cgccgctttc gcgagaacga
     2881 cggagaggct ttttacgaac tgttggacaa caatcgttcc cgaatcgagg aattgctcgc
     2941 gctctccgcc tcagacatcg gcagcaaaga agactgtgag gcatttgtgc gccgcaaact
     3001 ttccaactgg ctgcttcagc aatcttatgc tttcggcgta tggcacacag acggcgccaa
     3061 gcccatcggc tacgtcgaat tgttcaaaat agattggtct gtgccaaaat cgtgcatcgt
     3121 attttttgtg gataaggaat acgagagcaa aggcattatg acggaagtga tccgtgaaat
     3181 cgggcgttat gcgttttcac aactcaatat ggagcgcata gagttattca ccgccagcga
     3241 caattttccg gcgcagcggc tggcccgcaa atgtggtttc cgccgcgaag gcgacctgcg
     3301 cgcattcttc cgcaggccca gcggagaatt ggtggacgtc atgcttctcg ccgtcacgcg
     3361 ctgaaaacgg tcatggctca acacaacgac accggccagg caggcgaaga actcgctgcg
     3421 caattccttg aacaacaagg ctttcaaata ctccatcgca actggcgctt tggccgggcc
     3481 gaggtggaca ttgtagccat ggacggccct gcgctggtgc ttgtagaggt caaagcccgc
     3541 agttccgacc atttcggcag gccggaggac ttcgtttccg ccaaaaagca gcgttttctg
     3601 gcggaagccg cttccagata tatggaaatg tacggccacg attgggagat tcgcttcgat
     3661 gtggtggcgg tgctgttcaa cccggaaaca gaaatcactc atttccggga cgcctttttt
     3721 cctgaatggg gatgacccga atcttgatga ataaagcgta cctttgcacc gattttgaaa
     3781 tcgcctgcca catgcaagac aaactcgaag ccttcggaag gctgctcacc atcatggaca
     3841 ccctgcgcga acagtgcccc tgggaccgca aacaaaccct gcaaagcctg cgcaatctca
     3901 ccattgagga aacgtacgaa ctggcggatg ccatcctcga cgataacctc aacgacatca
     3961 aggaagagat cggcgacatt ctgctgcaca tggtttttta tgccaaaata gccgacgaaa
     4021 aaggcgcatt ccacatcggc gacgctatcc atgccgtttg cgacaagctt attgcccgcc
     4081 acccgcatat ctacggagac gtgcaggtga aagacgaaga agaggtgaag aaaaactggg
     4141 agcaactcaa actccgcgag ggcaaaaagt ctgtccttca aggtgttccg cgctctttgc
     4201 cggccatgat aaaggcatac cggatgcaag agaaaaccaa acaagtgggt tttgaatggg
     4261 agaacagcga gcaggtttgg gaaaaggtag aagaggaaat ctctgaattt cgcgaggcta
     4321 tgcaaactgg aatgtctcag gaaaaacggg aagaggagtt cggcgatgtc atcttttcac
     4381 tcatcaatta tgctcgtttt caaggcattg accccgaaac ggccttggaa cgggtcaaca
     4441 ataaattcaa aaaacgcttt gagtacatcg aggcccacgc gcctaccgac ttgcgcaata
     4501 tgacgctggc cgaaatggac gcgctctgga acgaagccaa aaccgcattt aactcctaag
     4561 cgtttattt
//
LOCUS       DAORLF010000400         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10389_length_4547_cov_2.248442, whole genome shotgun sequence.
ACCESSION   DAORLF010000400 DAORLF010000000
VERSION     DAORLF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10389_length_4547_cov_2.248442"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>353
                     /locus_tag="PK198_22460"
     CDS             23..>353
                     /locus_tag="PK198_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41581.1"
                     /translation="MFTLCFNALANTGATSVVFTGTPTTIEVINTALQDVPFNSISGT
                     VSFGSGGGGTPNPISLAVGSQSNVMNGEQVCLDVTAQNFSNINSFQAAITYNAALLQN
                     AVVSHFGP"
     assembly_gap    354..386
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            453..>1674
                     /locus_tag="PK198_22465"
     CDS             453..>1674
                     /locus_tag="PK198_22465"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRF41582.1"
                     /translation="MFRLCFTANAANGSTQVAVGSIVATNGANQFVPAVSAGGNVFFN
                     TQTGLTMSIGNASNVVQGQQLCLNVSAEDFTNILGLQFSINYNPALLLLTSVSNFGLP
                     ALSASENFGLPIPGGAGNTVPGIITFTWIGPGNMPVSRPNGTVVFTLCFNVLSNAENT
                     QVVFSNSPSVIEVINGQMQSIPFNGIPGSVFPQENCINAVSNAVRVTVRPDPAIGLLP
                     ESQTVCINSPITLTTQVSGGAGDCSFRWFSAPASTGPWTLIPDAVGDTYQPPTQQLGL
                     TYYLALLTCTGSGCGQPASNPAAVLVTDNLFGDEIRATPLACNVWRLSPVLPPNYFGP
                     INALWTLPDGSTSTDVQIIATQTGVYQLQISVPASPCQSYLARYINSNADECASIVGY
                     VRHDLDENCQPQTDE"
     assembly_gap    1675..1786
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1787..>2586
                     /locus_tag="PK198_22470"
     CDS             <1787..>2586
                     /locus_tag="PK198_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41583.1"
                     /translation="SLLPPSASWLLCADSYPVSLDQSGQVSQLDIPVKRIRPCPQLEA
                     QLSTLLLRRCFTSTYVVKICNVGTEIAESPVATLVLDDFLSYQSAQRTPSSIQGQTIT
                     WELPALAPGQCRQFNVYVLVSCNAVLGQSHCSTLSVIPDPLCVPTSPAWSGASLQVSG
                     ECTGSQARFRVRNAGIGDLDAPVPCIVIEDVVMLMHQPNTINHLSIGEEVVYDFPANG
                     STYFFSVGQAPHHPFGNPVTLALEGCGTNNAGTFSTGFVNQFPLQNTTT"
     assembly_gap    2587..2626
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2627..3336
                     /locus_tag="PK198_22475"
     CDS             <2627..3336
                     /locus_tag="PK198_22475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41584.1"
                     /translation="DPNDKQAVPVGYGPEHFIRAGDPLHYQIRFQNTGTDTAFTVIIR
                     DTLSPWLDMATLRPGPSSHDYKAHIDGERTLVFTFDNILLPDSTANLDGSQGFVDFFI
                     HTLDSIPLETRIENSAAIYFDFNEPVITNTVFHTIGRDFVRSATAVFQPDAPAAEWLL
                     MPNPAREEAWVVLKREVSGLKTAWLYDAFGRQVLRLPFEGDRCRLPLTDLRPGWYALQ
                     LTDAAGRSLGAGRLVVR"
     gene            complement(3417..4442)
                     /locus_tag="PK198_22480"
     CDS             complement(3417..4442)
                     /locus_tag="PK198_22480"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRF41585.1"
                     /translation="MKHTTFHTATAVALMCLIFTQCRPQSLDERMNYAAEHGILDLSG
                     LAIKEIPDEVWQMSGLTQLNLGQNQLETLPPDIGKLQQLESLILYDNRLQDLPGELAQ
                     LPKLKHLNIGKNQIRSLPDVVTKLPALAELTWTHSDLSVLPDALWAMSGLTHLHLNNN
                     KISSLPPGIGQLANLKGLFIFQNQLQSLPPEIGRLSQLQVISASSNQIRELPAEIGQL
                     RNLTMLELWGNQLTGLPASIGDLENLSSLQLNKNQLRQLPQELGRLRMLANLELHDNQ
                     LRTLPETVGGMASLQVLMLAHNPLENLPPQLAELPKLIGISISAGQGHLIPEALRTRS
                     GLKVVEE"
BASE COUNT          920 a         1216 c         1204 g         1022 t
ORIGIN      
        1 atccctttta aataatagca gattgtttac cctgtgtttc aatgcgttgg ccaatacggg
       61 cgctacctcc gtggtgttca ccggcacgcc cactactatt gaggtcatca atacggcgct
      121 gcaagatgtg ccgttcaaca gcatatcggg tacggtgtct ttcggcagtg gcggcggagg
      181 aacgcccaat ccgatcagcc ttgcagtggg tagtcagagc aatgtgatga atggcgagca
      241 ggtgtgtttg gatgtgacgg cgcaaaactt cagtaatatc aattcgtttc aggcggccat
      301 cacttacaat gcggccctgt tgcaaaatgc tgtggtgagc cattttggtc cggnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnacgc cgggtaccat atcagtgtat tggttcaatg
      421 cggcgcccca aactttaccg caaaacgagg ttttgtttcg actttgcttt acagccaatg
      481 cagccaatgg ttcgacacag gtagcggtag gaagtattgt cgctaccaac ggcgccaacc
      541 agtttgtgcc ggctgtcagc gcaggaggta atgtgttttt caatacgcaa accggcctca
      601 ccatgagcat cggcaacgcg agcaatgtgg tgcaggggca gcagttgtgc ctgaatgtga
      661 gcgccgaaga ttttacgaat attttgggat tgcaattcag tatcaactac aatccggcgc
      721 tgttgctact tacttcggtg agcaatttcg ggttgccggc tttatccgct tccgaaaatt
      781 ttggtttacc gatacccggc ggcgctggaa acacagtgcc gggtatcatc acctttactt
      841 ggattggccc gggcaacatg ccggtgagcc gccccaacgg gaccgttgtt tttacgctgt
      901 gtttcaatgt gttgtctaat gccgaaaata cacaggtcgt tttcagcaac tcgccatccg
      961 tcatagaggt catcaacggg caaatgcaaa gcatcccctt caacggcata cccggctcgg
     1021 tcttcccaca ggaaaactgt atcaatgccg tttctaatgc cgttcgtgtc accgtgcgcc
     1081 ccgaccccgc catcggcctg ctgcccgaaa gtcagactgt ctgtatcaac agccccatta
     1141 cactcaccac gcaggtgagc ggcggcgccg gcgattgcag tttccgatgg ttcagcgcac
     1201 cggcctccac aggcccttgg acacttatac ccgatgctgt gggagacacc taccagccgc
     1261 cgacccagca attggggctt acctattatt tggctctgct cacctgtacc ggcagcggct
     1321 gcggccaacc ggcatccaac ccggcggcgg tactcgttac cgacaatctt tttggagatg
     1381 aaatccgcgc tacgcctttg gcatgtaatg tgtggagact cagtcccgta ttgccgccca
     1441 attatttcgg ccccatcaat gccctgtgga cgctgcccga cggcagtacc agtacagatg
     1501 ttcaaatcat tgccacccaa accggcgtgt atcaattgca gatttcagta cccgcctcgc
     1561 cgtgccagtc ttacctcgcc cgctacatca actccaatgc cgacgagtgc gcctccatag
     1621 tggggtatgt gcgccacgat ctggatgaaa attgccagcc gcaaaccgac gaacnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcc tgctgccgcc
     1801 ctccgcttcc tggctcttgt gcgccgattc ctaccctgtt tcgctcgatc aatccgggca
     1861 ggtgagccaa cttgatattc cggtgaagcg catcaggccc tgcccgcaat tggaggcgca
     1921 gctttccacg ctcttgctga ggcgttgttt cacaagtact tatgtggtaa aaatttgcaa
     1981 tgtcggcact gaaattgcag aatcgccggt agccactttg gtgttggatg attttttgag
     2041 ctaccaaagt gcgcagcgca ctccgagttc catacagggc caaaccatca cctgggagtt
     2101 gccggcattg gcgccggggc agtgcaggca gtttaacgtc tatgtgttgg tgagctgcaa
     2161 cgccgtgctg ggacagtcgc attgctccac gctctccgtc atacccgatc cgctgtgcgt
     2221 gcccacttct cccgcatggt cgggcgccag tttgcaggta tcgggcgagt gtacgggcag
     2281 tcaggccaga ttccgggtgc gcaatgccgg cataggcgat ctggacgctc cggtgccctg
     2341 catcgtcatt gaagacgtgg tgatgctcat gcaccagccc aacaccatca atcatttgag
     2401 cattggcgaa gaggtggttt atgactttcc ggccaacggc tctacctact ttttttcggt
     2461 gggacaagcg ccgcaccacc ccttcggcaa ccccgtgacg ctggcgctgg aaggctgcgg
     2521 caccaacaat gcgggaacct tcagcaccgg ttttgtcaat cagtttccac tgcaaaacac
     2581 tacgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacga ccccaacgac
     2641 aagcaggccg tcccggtggg ctacgggccg gaacacttca tccgcgccgg cgatccgctg
     2701 cactaccaaa tccgcttcca aaataccggc accgacacgg ccttcaccgt catcatccgc
     2761 gatacgctct cgccctggct cgacatggcc acgctgcgtc ccggcccctc cagccacgat
     2821 tacaaggcgc acatagacgg cgagcgcacg ctggtcttta cgttcgacaa catcctgctg
     2881 cccgacagca ctgccaatct cgatggttct caaggttttg tggacttttt tatccatact
     2941 ttggacagca ttccgttgga aacgcgcatc gaaaacagcg ccgctattta tttcgatttc
     3001 aacgagccgg tcatcaccaa taccgttttt cacaccatcg gcagggattt tgtgcgctcg
     3061 gcgacggcgg tattccagcc cgatgcgccc gccgctgaat ggttgctgat gcccaatcct
     3121 gcccgggaag aggcgtgggt ggtactgaaa agagaagtgt ccggcctgaa aaccgcctgg
     3181 ctgtacgacg ctttcgggcg acaggtgttg cgcctgcctt tcgagggcga ccgctgccgc
     3241 ctgccattga cagatctaag gccgggctgg tacgcattgc aactgacgga tgcggccggg
     3301 cggagcttgg gcgcggggag attggtggtg aggtagggta ggggcgctaa atgaggccga
     3361 gggatgagcg tgccggggga gggcctcggc gcgagcgggg taaagattta ctacccctac
     3421 tcctcaacaa ctttcagccc cgaacgggtg cgcagagcct ccggtatcaa atgcccctgc
     3481 ccggcgctga tgctgatacc gatgagttta ggcagctctg ccaactgagg cggcaggttt
     3541 tccaaggggt tgtgcgccaa catcagtact tggagcgaag ccatacctcc gacggtttcc
     3601 ggcagggtgc gcaattggtt gtcatgcaat tccaagttgg cgagcatgcg caggcggccc
     3661 agttcctgcg gaagttggcg gagttgattt ttgttcaatt gcaaagaact cagattttcc
     3721 aaatctccga tggaggcggg aagcccggtg agttggttgc cccagagttc cagcatagtc
     3781 aggttgcgga gttgcccgat ttcagcagga agctcccgaa tctggttgga agatgcggag
     3841 atgacttgta attgactcaa tcggccgatt tcaggcggca atgactgaag ttggttttgg
     3901 aaaataaaca atcctttcag attggccaat tgtccaattc ctggaggtaa actgctgatt
     3961 ttgttgttgt tgaggtgcaa gtgcgtcaac ccggacatcg cccagagcgc atcgggcagc
     4021 acgctgagat cgctatgcgt ccaggtcaac tctgccaacg cgggcaactt ggtcaccaca
     4081 tcgggcaagg aacgtatctg gtttttgccg atattcagat gcttcagttt gggaagttgg
     4141 gctagttcgc caggcaagtc ctgcagccgg ttgtcgtaca ggatcagcga ttccaactgc
     4201 tgtagtttgc caatgtcggg cggcagcgtt tccaattggt tttggcccag attcaactga
     4261 gtaaggccgc tcatttgcca cacttcgtca ggtatttctt tgatagccag tccactaaga
     4321 tcgagtattc catgttctgc ggcatagttc atgcgttcgt ccaaagattg cgggcggcat
     4381 tgtgtgaaga tcagacacat taaagcaact gctgttgcgg tatggaaagt ggtgtgtttc
     4441 attttatcaa aggtgattat tctgcaaaaa gcacgatgca attcggcaaa gcccggcgga
     4501 ttttctccac ctcagcagcc ggcagcggat tgcggtggag cgaaagc
//
LOCUS       DAORLF010000401         4539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10413_length_4539_cov_2.075379, whole genome shotgun sequence.
ACCESSION   DAORLF010000401 DAORLF010000000
VERSION     DAORLF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10413_length_4539_cov_2.075379"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PK198_22485"
     CDS             complement(<1..738)
                     /locus_tag="PK198_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41586.1"
                     /translation="MKTNHLSTALLSLGLFLLAAACSKESADISNDPGQAGSITRFAV
                     HNGFMYALNPNQVRTFSLENPDKPQLVHTLTTDYGLETIIIYDNTIFLGSRTALYILD
                     IANPAAPSILSQTQRGEFLFGGCDPVVVKDNFAYSTIKIIENVCGRFASQSALMVYDV
                     SNRNAPANVGVHFMSLPNGLGYKDNYLFVCDEGTDRLEVFDISDPMNTRTTSFSVAVA
                     DPVDLIVKGDKMIVSSKTEFRFFDISNI"
     gene            complement(754..>1291)
                     /locus_tag="PK198_22490"
     CDS             complement(754..>1291)
                     /locus_tag="PK198_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41587.1"
                     /translation="QFRSLSFWFLPAVLALWLFSGCDEDIEPFSGVGKRPVYIPLAQL
                     SDIKSLPPRPIVLSGSIFLRDSFFFMLEQRQGIHVFNVADPGNPVAVAFWQIPAIGDF
                     SMSGNRLFVDSWRDLVTIDISDIHNIRSIDRQTGLFDPLLYPPLFNGIFECVDESKGA
                     VAGWEDAFLTEARCRTFN"
     assembly_gap    1292..1305
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1398..>1684)
                     /locus_tag="PK198_22495"
     CDS             complement(1398..>1684)
                     /locus_tag="PK198_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41588.1"
                     /translation="FVLQIWSEFKYGHCSPLILQYLREARYTAHILCRPDIPWTFDPL
                     REHPEQREVLFGLYLKALQQAGAMFIIAEGAEEQRRQAAEDFVREMQKHL"
     assembly_gap    1685..1789
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1947..2756
                     /locus_tag="PK198_22500"
     CDS             1947..2756
                     /locus_tag="PK198_22500"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRF41589.1"
                     /translation="MAETTAIMETTQEPTECKTCAAPLSGVYCSQCGQRLITRRITLR
                     ALFSELISLITNTDRGLLHTFWMLLKNPGKVVHDYIGGSTVQYASPLRYTLVGTALVT
                     IVFIGMGVYDRQMAEMDSMMHQGHTPEAEMATNQIYALMRQYYQLISILFIPIFAWIT
                     RLLFRKSRLNYAEHLVTNAYFNTQATLLMTPFLLAVALTSVKMSSMMWVSMLASVAYY
                     TLALRSAFDQKWLPAFLKSVLVYVLGTIIYTLVMGLLAIPISIYMVLKAKM"
     gene            2767..3354
                     /locus_tag="PK198_22505"
     CDS             2767..3354
                     /locus_tag="PK198_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41590.1"
                     /translation="MHDLTPHHRWQAAYIADEDERSPFYRQDKDRMHYTHAVYNHYIH
                     PDWDAFGSSTLYMKLLYADYVEGYAIMEFIGEWNDTLHNDVMYLKRKVVDLMLPEGIH
                     KFILICENVLNFHGSDDCYYEEWQEEAADEGGWICFVNTLQHVADEMEDTRLQFFVHF
                     GEAFNNIEWRLQKPRRVFQMVEERVYRGSGQLRSG"
     gene            complement(3359..>4539)
                     /locus_tag="PK198_22510"
     CDS             complement(3359..>4539)
                     /locus_tag="PK198_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41591.1"
                     /translation="QWGNRPLEDNWRRSAKGFSSEAENAGPENAASTASETTSAGPLT
                     DAEIKELLGNFPSTEEEVAQVHLKLKEAMFNLGKLYRDKLNRLDKSVEILERLNQRFP
                     ANNYELESWYLLYLLFTDMNNSPKAKEYADKIINKYPTSVYGKVLQDPGYVQELADER
                     RRLNIFYDQAYAKFQNEQYQQAFDQCQQAKEKFGAGNPLQPKFALLSAMCMGNLQGKN
                     AYVAGLNEVMSKYPNTPEQTRAREMLRLISGASAALPGGQTDAILPEGELYKDEPDDL
                     HYVIIYLDKTAPIDKSRNQLSDYNQKYHDLDKLRIANVFLGASNEIPVLVLRRFKDKK
                     ESMRYIQGAEKNAQDFLNKSEYGFTLLSISQNNYRNLLRDKRTEVYQEFFNQFYKPGR
                     "
BASE COUNT         1026 a         1074 c         1251 g         1069 t
ORIGIN      
        1 gatattgctg atgtcgaaaa agcggaactc cgttttggaa gacacgatca ttttgtcgcc
       61 tttcacaatg aggtcaacgg gatcggccac ggctacagag aaggaggtgg ttcgggtgtt
      121 catcgggtcg gaaatgtcga acacttccag ccggtcggtt ccttcgtcgc acacaaagag
      181 gtaattgtct ttgtatccca gaccattggg caggctcata aagtggacgc ctacattggc
      241 aggggcattg cgattgctga catcgtacac catcagcgcg ctttgggagg cgaaacgtcc
      301 gcatacgttt tcaataattt tgatggtgga ataggcaaaa ttgtctttga ctaccaccgg
      361 atcgcagccg ccgaaaagga attctccacg ttgggtttgc gagaggatag acggcgcggc
      421 gggattggcg atgtctaaaa tgtacagtgc cgtgcgcgag ccgaggaaaa tggtgttgtc
      481 gtaaatgatg atggtttcca gaccgtagtc ggtagtcagg gtgtgtacca actgcggctt
      541 gtcggggttt tcgaggctga aagtgcgcac ctgattgggg ttgagcgcat acatgaaccc
      601 gttgtgaacc gcaaaacgtg taatggagcc tgcctggccg ggatcattgc tgatatcggc
      661 agattctttg gagcaggctg cggcgagcaa aaagaggccg aggctgagaa gagcggtgga
      721 taagtgattg gttttcatag ttatattatt gatttaatta aacgttcggc agcgggcctc
      781 ggtgaggaag gcatcttccc agccggcgac ggcgcctttg ctttcgtcca cacattcaaa
      841 aatgccgttg aaaagcggcg gataaagcag cgggtcgaag aggccggttt ggcggtctat
      901 gctgcggatg ttgtgaatgt cggaaatatc tatggtgacc aagtcgcgcc aactatccac
      961 aaaaaggcga ttgccggaca tgctgaaatc gccgatggcc ggaatttgcc agaaggcgac
     1021 ggcgaccgga ttgcccgggt ctgccacgtt gaaaacatgg atgccctggc gctgttcgag
     1081 cataaaaaag aagctgtcgc gcaggaaaat agagccgctc agcactatgg ggcggggagg
     1141 caggcttttg atgtcgctga gttgagccaa cggaatgtaa accggtcttt tgcccacgcc
     1201 gctgaaaggc tcaatatctt catcgcagcc ggaaaacagc cacagcgcca aaacggcggg
     1261 caaaaaccaa aatgacagag atcgaaactg cnnnnnnnnn nnnnngcata acaggcggta
     1321 ttttgcaaca aaggtgcatg aaagaaacga ggccaatcgt tccagcgctg ctttgatcgg
     1381 caaattttac aaatagttta cagatgtttt tgcatctccc tcacaaaatc ctctgccgcc
     1441 tgcctccgct gctcctctgc cccctccgct atgatgaaca ttgccccggc ctgttgcaat
     1501 gccttgaggt acaatccgaa taaaacttcg cgttgttcgg ggtgctcgcg cagcggatcg
     1561 aacgtccagg ggatgtcggg gcgacaaagg atgtgggcgg tgtagcgggc ctcgcgcagg
     1621 tattgcaaaa tgagcggcga gcaatggccg tatttgaatt ccgaccagat ttggagcaca
     1681 aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gctatggagt
     1801 ctaaatacag gcgggcatat tcctgcacca aaggggcgcc gaaacgcgca gccacatact
     1861 gcgccagagt ggttttgccg ctggattccg ggccggtgag ggctattttg atcatgtagc
     1921 aaaggtaatg tcgaaaactc atacctttgg ccgaaactac tgccatcatg gaaacaacac
     1981 aggaaccaac cgaatgcaaa acctgcgccg cgcccttgtc gggcgtctat tgcagccaat
     2041 gcggccagag actcatcacc cggcgcatca cgcttcgggc gcttttttca gaacttatca
     2101 gcctgattac caatacagac cggggtctgc tgcacacgtt ctggatgctg ctgaaaaatc
     2161 ccggcaaagt agtgcatgac tacatcggcg gcagcacggt gcagtatgcc tctccgctgc
     2221 gatatacgct ggtaggcacg gccctcgtga ccatcgtttt tatcggaatg ggggtgtatg
     2281 accggcaaat ggcggaaatg gacagcatga tgcaccaagg gcacaccccg gaggccgaaa
     2341 tggcgacgaa tcagatatac gccctgatga gacagtatta tcagctcatt tccatcctgt
     2401 tcatacccat ttttgcatgg attacccgac tgttgttccg gaagtcgcgg ttgaactatg
     2461 ccgaacattt agtgacgaat gcctatttca acacccaggc cacgctgttg atgacgccgt
     2521 ttttgctggc cgttgcgttg acttcagtta aaatgtcttc catgatgtgg gtaagtatgc
     2581 tggccagtgt ggcatactac acgttggccc tgcgcagcgc tttcgatcaa aagtggctgc
     2641 cggcatttct caagtcggtg ctggtgtatg tgcttggcac catcatttat accctggtaa
     2701 tggggttact cgcgattcca ataagcattt atatggtttt gaaagcaaaa atgtgatttc
     2761 aataagatgc acgatctgac gccgcaccac cgctggcagg ctgcctacat agccgatgaa
     2821 gacgagcgat ccccgtttta ccggcaagac aaagaccgca tgcactacac gcacgccgtt
     2881 tacaaccatt acatccatcc ggactgggac gcgttcggct cttctacctt gtatatgaag
     2941 ctcttgtacg ccgattatgt ggaaggctac gccatcatgg agtttattgg cgagtggaac
     3001 gacaccctgc acaacgacgt gatgtacctc aagcgcaaag tcgtggacct gatgctgccg
     3061 gagggcattc acaagttcat tctcatttgt gaaaacgtgc tcaatttcca cggctccgac
     3121 gactgctatt acgaagaatg gcaggaggaa gctgcggacg agggcggctg gatttgtttt
     3181 gtgaatacac tgcaacacgt cgccgatgaa atggaggaca cgaggctaca gtttttcgtg
     3241 catttcggcg aggcgttcaa caacatagaa tggcggctgc aaaaaccccg gcgggtcttc
     3301 cagatggtgg aggagcgcgt gtatcggggc agcgggcagt tgcgaagcgg atgaattttt
     3361 atctccccgg cttgtaaaac tgattaaaaa actcctgata cacctctgtg cgcttgtcgc
     3421 ggagcagatt gcggtagttg ttttgagaaa tagacaataa tgtgaaaccg tactccgact
     3481 tattgagaaa atcctgcgca ttcttttctg cgccctgaat atagcgcata gattcctttt
     3541 tgtctttaaa acggcggagc accaataccg gaatctcatt ggacgcgccg aggaacacgt
     3601 tggcaatgcg gagtttgtcc aaatcgtggt acttctggtt gtagtcggag agttggttgc
     3661 gcgatttgtc tatgggcgca gttttgtcca aatagataat gacatagtga aggtcgtccg
     3721 gttcgtcttt gtagagttca ccttcgggaa gtattgcgtc ggtttgtccg cccggcaatg
     3781 ccgccgaggc gccgctgatg aggcggagca tttcgcgggc gcgggtttgt tcgggcgtat
     3841 tgggatactt ggacatgacc tcgttgagac ctgcgacgta ggcgtttttg ccttgtaaat
     3901 tgcccatgca catggcgctg aggagggcaa acttaggctg aagcggattg cccgcgccga
     3961 acttttcttt tgcctgctgg cactggtcaa atgcttgctg gtactgctcg ttctggaatt
     4021 tggcgtatgc ctgatcgtaa aaaatattga ggcggcggcg ctcgtcggca agttcctgca
     4081 catagcccgg gtcttgcagc accttgccgt aaacggaggt cggatactta ttgatgattt
     4141 tgtcggcgta ttccttggcc ttggggctgt tgttcatgtc ggtgaacagc aggtacagca
     4201 gataccagga ctccaactcg tagttgttgg ccgggaagcg ctgattgagc ctttccaaaa
     4261 tttccaccga cttgtcgagg cgattgagct tgtcgcgata caacttgccg aggttaaaca
     4321 tagcctcttt gagcttgaga tgcacttggg ccacctcttc ctctgtggac gggaaattgc
     4381 ccagcagttc tttgatctct gcatcggtga gtggcccggc actggttgtt tccgatgccg
     4441 tagaagccgc attttccgga ccagcattct ccgcttcgct gctgaaacct ttagccgagc
     4501 ggcgccagtt gtcttccaag gggcggttgc cccattggc
//
LOCUS       DAORLF010000402         4536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10427_length_4536_cov_2.031466, whole genome shotgun sequence.
ACCESSION   DAORLF010000402 DAORLF010000000
VERSION     DAORLF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_4536_cov_2.031466"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK198_22515"
     CDS             complement(<1..325)
                     /locus_tag="PK198_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HRF41592.1"
                     /translation="MEGIPYLNEHLWPGRIGHAGILLAFTAAILSAFSYFMSVQRADY
                     ADEAATWKKIGRGAFLGHALGVFTVVGMLFYVMIREYTEYQYVWAHVSDDLPLKYIFS
                     AFWEGQ"
     gene            complement(325..726)
                     /locus_tag="PK198_22520"
     CDS             complement(325..726)
                     /locus_tag="PK198_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRF41593.1"
                     /translation="MKKIYVIVLVMIVAGIALLINAAGDTSTYSTFEDAARSGGKVKI
                     AGQLAKDKEMHYDPQKDPNYFTFYLTDTEGNERKVVLLREKPQDFEMSEQIVLTGKMK
                     GDEFVATEMLMKCPSKYKDEEIYINAEKENS"
     gene            1083..1802
                     /locus_tag="PK198_22525"
     CDS             1083..1802
                     /locus_tag="PK198_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRF41594.1"
                     /translation="MGWLKNFLTSSIGKKLVMSLTGLFLILFLVVHLIGNLQLLQFMA
                     HDSAGEIGRAFNEYALKMTTFLPIKVVSYGLYAFILIHAVQGWMLWRQNAAARGPVRY
                     AVSDLRAVNTNARVATRMGWLGTIIFIFIILHMYQFWLQMKMGNLPMANYGDGDVKDL
                     YTIVAEAYTNPLYVILYVVSMFFIGVHLWHGFQSAFQTLGLNHNKYTPLIHKLGKGYS
                     ILVPLGFAIIPVVMYLQSLNA"
     gene            1817..>2014
                     /locus_tag="PK198_22530"
     CDS             1817..>2014
                     /locus_tag="PK198_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF41595.1"
                     /translation="MKLDGKIPAGSLAEKWTKYRSTVSLVAPHNKMRLEVIVIGSGLA
                     GASAAATLGELGYNVKCFTFHD"
     assembly_gap    2015..2106
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2107..3829
                     /locus_tag="PK198_22535"
     CDS             <2107..3829
                     /locus_tag="PK198_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRF41596.1"
                     /translation="YRLFYDTIKGGDYRAREANVHRLAEVSTSIIDQCVAQGVPFARE
                     YGGLLDTRSFGGTQVQRTFYAKGQTGQQLLIGAYQALSRQISLGKVKMYNRHEMLDLV
                     TIDGKARGIIARNLVSGKLERFGAHAVVLATGGYGNVFYLSTNAMNCNVTAAWRTVRR
                     GALMANPCFTQIHPTCIPVSGDYQSKLTLMSESLRNDGRVWVPKKQEDAQAIREGRKK
                     GNDIPEADRDYFLERKYPAFGNLVPRDVASRASKSMCDAGFGVAPTGLAVFMDFSAAI
                     ERYGRSRASSLGLANADKATIRALGEKVIDEKYDNLFAMYEKITGENAKKMPMKIYPA
                     VHYTMGGLWVDYNLETNIPGLFATGECNFSDHGANRLGASALMQGLADGYFVLPYTIG
                     TFLANEIKTPTIDTNAPEFAAVESETRARLLGFLSVNGKQSVESFHRRLGRIMWEYCG
                     MARNAEGLAKARKMIQELYEEFKADVFVPGQLEEFNPELEKAMRVADFMELGELMIVD
                     ALNRNESCGGHFRTEYQTEEGEAQRNDEEYAYVAAWEYKGIGQEPVMHREDLVFDVVK
                     PTSRSYK"
     gene            3912..>4536
                     /locus_tag="PK198_22540"
     CDS             3912..>4536
                     /locus_tag="PK198_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRF41597.1"
                     /translation="MKITLKVWRQKDSADKGRFETYTLNNVSEHMSFLEMFDVLNEEL
                     IQKKEVPVAFEHDCREGICGACSMVINGRPHGPMQGTTTCQLHMRHFNDGDTIVVEPF
                     RARAFPVLRDLVVDRSAFDRIIQSGGFISVNTGQAQDANAIPVNRDAAEASFSAAACI
                     GCGACVAACPNASAMLFVSAKTAHLNLLPQGQIEAKRRVQAMVKQMDG"
BASE COUNT         1038 a         1194 c         1209 g         1003 t
ORIGIN      
        1 cctgtccttc ccagaatgcc gagaagatgt acttgagcgg caggtcgtca gacacatgcg
       61 cccaaacata ctgatactca gtgtattccc tgatcatgac gtaaaacaac atgcccacca
      121 ctgtaaatac gcccaaggcg tggcccagaa aagcgccccg gcctattttt ttccaggtag
      181 cggcctcatc ggcgtagtcg gcgcgttgaa cggacatgaa gtaagaaaag gcgctgagta
      241 tggcggctgt gaaagccagt aagatacctg cgtgcccgat gcggcccggc cacaggtgtt
      301 cattcaaata cggaatcccc tccattagct gttttctttc tctgcgttga tgtaaatctc
      361 ctcgtctttg tatttggagg ggcatttcat gagcatctcg gtggctacaa actcgtcgcc
      421 cttcatttta ccggtgagga cgatttgctc cgacatttca aagtcctggg gtttctcacg
      481 caggagcacc actttgcgct cgttgccctc ggtatcggtg agatagaagg tgaaatagtt
      541 ggggtctttt tgcgggtcgt agtgcatttc cttgtctttg gccaattggc cggcaatttt
      601 caccttgccg ccgctgcggg ctgcgtcttc aaaggtggaa taagtgctgg tgtcgccggc
      661 ggcgttgatg agcagcgcga tgcctgcaac gatcatcacc aatacgatca catatatttt
      721 cttcatggtc ggacggtttt ttgtattcag ggcggcaaag gtaagacata agcggcgaac
      781 agatacattt taccccggtt atcgcacagt ctttcctttg ttgtacaaga caacaaacaa
      841 ggcggagcgg ggtttggcgg gggcggggat tttttgggat gggggtatgt tttaaattgt
      901 tgggtgactg gtggatggaa tgccctttca atgtagaaga attctaaata aatttttctt
      961 tccccttcct gctgaacatc cgttgggcaa gtcgccttta taccatactt ttgcgcgtcg
     1021 aaaaaatggg cgatgagttc cgtttttcac cgcaaaacaa aacgataaaa acagactgac
     1081 gtatgggttg gctaaaaaac ttcctgacct cttccatcgg gaaaaagctc gtaatgagcc
     1141 tcacagggtt gtttctgatc ctgtttctgg tagtacattt aattggcaac ctgcaattgt
     1201 tgcaattcat ggcacacgac tcggcaggtg aaatcggcag ggcattcaac gaatatgcgc
     1261 tgaaaatgac cactttccta cccatcaaag tggtgtctta cggactgtat gccttcatcc
     1321 tcatccacgc tgttcaaggc tggatgctgt ggcggcaaaa cgcagcggcc cggggtccgg
     1381 tgcgatacgc cgtgagcgac ctgcgggcag taaacaccaa cgccagagtg gccacccgta
     1441 tgggctggtt gggtaccatc atcttcattt tcatcatatt gcacatgtat cagttctggc
     1501 tgcaaatgaa aatgggcaac ctgccgatgg ccaactatgg cgatggggat gtaaaagacc
     1561 tctacaccat tgtcgccgag gcttatacca atcccttgta tgtcatactc tatgtggttt
     1621 cgatgttctt catcggcgta cacctgtggc acggcttcca gagtgcattc cagacgctgg
     1681 gcctcaacca caataaatac acgccgctca ttcacaagct gggcaaaggg tactccatcc
     1741 ttgttccgct cgggttcgcc atcattccgg tggtgatgta tctccaatct ttgaatgctt
     1801 aaaatcttgc acaaccatga agctcgatgg aaaaatacct gccggatcac ttgctgaaaa
     1861 atggacgaaa taccgctcca cagtatctct ggtagctcct cacaataaaa tgcgcttgga
     1921 agtcatcgtc ataggttccg gtttggccgg cgcttcggcg gcagccaccc tcggcgaatt
     1981 gggttacaac gtgaagtgtt ttaccttcca cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnngtat cgcctttttt acgacaccat caaaggcggc gactaccgcg cccgcgaagc
     2161 caatgtgcat cgcttggccg aagtgtccac cagcatcata gaccagtgtg tggcgcaggg
     2221 cgtgcctttt gcccgagagt acggcggcct gctcgatacc cgctccttcg gtggtacgca
     2281 ggtgcagcgc actttttatg ccaaaggcca aacaggccag cagttgctca tcggcgctta
     2341 tcaggcttta tcgcgccaga tcagcctcgg caaggtgaaa atgtacaacc gccacgaaat
     2401 gctcgatttg gtgacgattg acggcaaagc ccgcggcatc attgctcgca acttggtaag
     2461 cggtaagctc gaacgctttg gcgctcatgc agttgtgttg gctaccggtg gttacggcaa
     2521 cgttttctac ctctctacca acgccatgaa ctgcaacgtg accgccgcct ggcgcacggt
     2581 gcggcgcggc gctctgatgg ccaatccctg cttcacgcaa attcacccca cctgcatccc
     2641 cgtttcgggc gactaccagt cgaagctcac cctcatgtcg gagagcttgc gcaacgacgg
     2701 gcgcgtgtgg gtacccaaaa agcaggaaga cgctcaggcc atccgcgagg gccgcaaaaa
     2761 aggcaacgac attcccgaag ccgaccgcga ttacttcctc gagcgcaaat acccggcttt
     2821 cggcaatctc gtgccccgcg acgtagcctc ccgcgcctcc aaatccatgt gcgatgcggg
     2881 cttcggcgta gcgcccaccg gtctggccgt attcatggac ttctctgccg ccattgagcg
     2941 ctatggccgt tcgcgggcaa gctcgttggg ccttgccaat gccgacaagg ctaccatcag
     3001 ggcgctgggc gaaaaggtga ttgacgaaaa gtacgacaac ctcttcgcca tgtacgagaa
     3061 gatcaccgga gagaatgcca aaaaaatgcc gatgaaaatt taccccgccg ttcactacac
     3121 gatgggcgga ctgtgggtag attacaatct ggaaaccaat atccccggcc tgtttgctac
     3181 cggcgagtgt aatttcagcg atcacggagc caaccgcctc ggcgcttcgg cgctcatgca
     3241 gggcttggcc gacggctact tcgtattgcc ttacaccatc ggtactttct tagccaacga
     3301 gatcaaaacg ccgaccatag ataccaacgc gccggaattt gcagccgtag agagcgaaac
     3361 ccgcgcccgt ctgctcggct tcctttcggt gaacggcaaa cagtcggtcg aaagcttcca
     3421 ccgccgtttg ggccgcatca tgtgggaata ctgcggtatg gcccgcaatg ccgagggttt
     3481 ggcgaaagcc agaaaaatga ttcaggaatt gtacgaagag tttaaagccg acgtgtttgt
     3541 gcccggccaa ttggaagagt tcaacccgga attggaaaaa gccatgcgcg tggccgactt
     3601 catggaattg ggtgaactga tgatcgtgga tgcactgaat cgcaatgaat cttgcggagg
     3661 acacttccgc accgagtatc agacggagga aggagaggcg caacgcaacg acgaggagta
     3721 cgcttatgtg gctgcctggg aatacaaagg cattgggcag gagccggtga tgcaccgcga
     3781 agaccttgtg tttgatgtgg tgaaaccgac gtcgaggagc tacaagtaag catagggcat
     3841 ggggcggaga gcaaagagga tagggcaaag agcatagagt tcatcagcaa aattgattta
     3901 aaaaaaggat catgaagatc acactcaaag tctggagaca aaaagacagc gccgataaag
     3961 gccgcttcga aacttatacg ctcaacaacg tgagcgagca catgtcgttt ctggagatgt
     4021 tcgacgtgct gaacgaagag ctgattcaga aaaaagaagt gccggtagca tttgagcacg
     4081 actgccgcga gggcatttgc ggggcatgca gcatggtcat caacggccgt ccgcacggcc
     4141 cgatgcaagg cactaccacc tgccaattgc acatgcgcca cttcaacgac ggcgacacca
     4201 tcgtagtgga acccttccgt gcgcgcgctt tcccggtgct gcgcgacttg gtggtggacc
     4261 gctctgcctt cgaccgcatc attcaatcgg gcggcttcat ttccgtcaac acgggtcagg
     4321 cgcaggatgc caatgccatt ccggtgaatc gcgacgctgc cgaggcttcg ttcagcgcgg
     4381 cggcttgcat cggttgcggc gcctgcgtgg cggcttgtcc caacgcatcg gccatgctgt
     4441 tcgtatcggc caagacggcg catctcaatc tcctgccgca gggccagatc gaggccaagc
     4501 gccgggtaca ggctatggtg aaacaaatgg acggcg
//
LOCUS       DAORLF010000403         4533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10438_length_4533_cov_2.052702, whole genome shotgun sequence.
ACCESSION   DAORLF010000403 DAORLF010000000
VERSION     DAORLF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_4533_cov_2.052702"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK198_22545"
     CDS             <1..339
                     /locus_tag="PK198_22545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF41598.1"
                     /translation="EIGHLFDLEPLWYARALQIIEGRPRLLEADLSNRKTHEAPHDQV
                     ETALLISLFAAERTKLMDVFRQATELELEKSSLHPRLETPMRLIDLAFFIAEHDDHHL
                     AKITSLLQQA"
     gene            complement(387..1127)
                     /gene="bla"
                     /locus_tag="PK198_22550"
     CDS             complement(387..1127)
                     /gene="bla"
                     /locus_tag="PK198_22550"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HRF41599.1"
                     /translation="MKFFLIFLFLIALSGCSVPAPGLQPLESKSLKIEALTEGVSVHI
                     SYLDTEQVGFVACNGMVYVRGEEAIVFDTPTDNAGSEKLIRWIEKRWKKRIKAVVINH
                     FHEDCLGGLAAFHAHGIPSYSNRLTIELARATGEPVLPQNGFDETLTLTCGDRSVINR
                     FFGAGHTLDNIVSYLPAEKALFGGCLVKAMNANKGNLADADTARWSATIEQVQSAFPN
                     VKHVIPGHGAAGGPELLDFTARLFRLEK"
     gene            1221..>2415
                     /locus_tag="PK198_22555"
     CDS             1221..>2415
                     /locus_tag="PK198_22555"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRF41600.1"
                     /translation="MNREFLLNIAFLIGINLLIKPLYIFGIDRGVQNALPPGDYGYYF
                     VLFNFTWLFQIVNDFGIAYYNSRNLAQHPQLISKYFPNLLMLKAALTLLFAVFIFIAS
                     AAWGYAPWSNALLWFICLNQALTSLVLFLRSNISGLGMYRVDSGLSALDRLLVILIMG
                     AVLWVLPIELRIEHFVWSQTAALSLTGLVAFGVLRGRLGQWQFRWRPAFLRVFFRRSF
                     PYALAVFLMTLYTRVDAVMIERMLPGGKEEAGIYAAAYRLLDAANIIGFLFAGLLLPM
                     FARLLKAGSGADKLAHLSFRILWAMAIPLALFCWYWGEEIMRLLYHRADAYYGEVLGV
                     LMLSFIAMCGMYVYGTLLTANESLRRMNLIFAAGLALNIALNWALIPTHQALGAAAAT
                     SATQLV"
     assembly_gap    2416..2531
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2873..2894
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2895..>4533
                     /locus_tag="PK198_22560"
     CDS             <2895..>4533
                     /locus_tag="PK198_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRF41601.1"
                     /translation="VVHPPGAPVFLLIGRMFTWVAEMISDDPADIAVSINLLSALCSA
                     AAAMLVAWTAMIFGKFSLTGSREAETNLGQNIALVAAGLAAGLATAFSTSIWFSAVEG
                     EVYAMSTFFTALTLWAMVKWYHLPDTPQSDRWVLFAVYSAGLSMGVHLLSLLTFPALA
                     MLYYFKKYKEHTFMGMALAALVGVVFIVGVQSFVIVGIPSLWGAFDIMMVNGLGMPFH
                     SGLVPTILIIGGYIALMTLILQEKITSRAPMFVFSGVMFLFSAICTMGFTEYEGVKFL
                     WLGTLLTLITAFAQDALVQRRRDAQMILMGATLVIVSFSTIGVVVIRANAQTPVNMNA
                     PTDATRLIPYLNREQYGERALVKGPHFMASPDGNKSKYKPRFGPVGDRYELIDEKITY
                     GFKSSDEMLFPRIGDFLSDDRKGLYKAWLGLDPEKPLPPGRPNQADNISFLWNYQINW
                     MYWRYFMWNFAGKQNDVQGIHMGNVRDGNWITGIGFYDNLRLGDQKYMPDSLKHNKAN
                     NKLFALPFILGLLGLFYQARKDKKDALITGLLFFFTGI"
BASE COUNT          902 a         1227 c         1160 g         1106 t
ORIGIN      
        1 gaaataggcc acctcttcga tctggagcca ttgtggtatg cgcgcgcatt gcaaatcata
       61 gaaggccggc cgcgtctgct ggaagcggat ctcagcaata ggaaaaccca tgaggctccg
      121 cacgatcagg ttgaaacggc cctgctgata agcctttttg ctgcggagag gacaaaactg
      181 atggatgttt ttagacaggc tacggagctt gaactggaaa aatcatcgtt gcacccgcgc
      241 ctcgaaacgc ctatgcggtt gattgatctg gcgtttttta tcgctgagca cgacgatcac
      301 caccttgcca aaataacaag tttgctgcag caggcgtagc catagtggcg gagcaaaata
      361 gatttagcga gacgccgagg tgcccatcat ttttccaaac ggaaaagccg ggctgtaaaa
      421 tcgagcaatt ccgggccacc ggccgccccg tgtccgggaa taacgtgttt tacattggga
      481 aatgcgcttt gcacctgctc tatggttgcc gaccagcgcg ccgtatcggc atcggcgagg
      541 ttgcccttat tggcattcat ggctttgacg aggcagccgc cgaagagcgc tttttctgcc
      601 ggaaggtagg acactatgtt gtcgagggtg tgccctgctc cgaaaaaacg attgatgacg
      661 ctgcggtcgc cgcaagtcag ggtgagggtt tcgtcaaacc cgttttgtgg caacaccggt
      721 tctcctgtgg cacgcgccag ttctatggtc agacggttgg aataagaagg aatcccatgc
      781 gcatgaaaag cagccagccc tcccaggcag tcttcgtgaa aatggttgat cacaacagct
      841 ttgatgcgct ttttccagcg tttttctatc caccggatga gcttttccga gccggcattg
      901 tcggtcggag tgtcaaaaac aatagcttcc tcacctcgaa cgtacaccat gccattgcag
      961 gccacaaatc caacctgctc ggtgtcgagg taggagatat gcaccgacac gccctctgtc
     1021 aatgcttcta ttttcagtga tttcgattcg agaggctgca aacctggagc aggaacagag
     1081 cagcccgaaa gtgcaatcag gaagagaaat atcaggaaaa atttcatcgg atagaatttt
     1141 acaaatgtag cacggtagtt gttgccatac agcgggatgc aagatacgcc taagtcaaaa
     1201 aaaatcccca actttccccc atgaaccgcg aatttctgct caacatcgct tttctcatcg
     1261 gcatcaacct gttgatcaag cctttgtaca tcttcggcat tgatcgcggg gtgcaaaatg
     1321 cgctgccgcc cggcgactac ggttactatt tcgtgctgtt caattttacc tggctgttcc
     1381 agatcgtcaa cgatttcggc attgcgtact acaacagccg caacttagcc caacacccgc
     1441 aactcatcag caaatacttc cccaatctgc tgatgctcaa ggccgcgctc acgctgctct
     1501 ttgcggtatt catttttata gcctccgctg cctggggcta cgcgccctgg agcaacgccc
     1561 tgctgtggtt catctgtctc aatcaggcgc tgacttcttt ggtacttttt ttgcgctcca
     1621 acatatccgg gctgggaatg taccgggttg acagcgggct gtcggcctta gaccggctat
     1681 tggtcatcct catcatgggc gcagtgttgt gggtgctgcc catcgagctg cgcatcgagc
     1741 attttgtgtg gtctcaaacg gcggccctct cgctcaccgg actggttgct ttcggcgtgc
     1801 tgcgcggccg gctggggcaa tggcaattcc gctggcgccc ggcgtttttg agggtgtttt
     1861 tccggcgcag tttcccctac gcattggccg ttttcctgat gaccctctac acccgcgtgg
     1921 acgccgtgat gatagagaga atgctgcccg gcggcaaaga ggaagccggt atttacgccg
     1981 ccgcctatcg cctgttggac gccgccaaca tcatcgggtt tttatttgcc ggactcctgc
     2041 tgcccatgtt tgcccgcctg ctcaaagcag gcagcggcgc cgacaaactg gcccacctca
     2101 gtttcaggat tttgtgggcc atggccattc ctttggcctt gttttgctgg tattggggcg
     2161 aggagatcat gcggctgctc taccatcgcg ccgatgcgta ttatggagag gtgctgggcg
     2221 tgctcatgct cagtttcatc gccatgtgcg gcatgtatgt gtatggcacc ctgctgacgg
     2281 ccaatgaaag tctgcggcgc atgaatctca tttttgccgc cgggctggcg ctcaacattg
     2341 cgctcaactg ggcgctcatt cccacgcacc aagccctcgg cgcggcagcc gctacttccg
     2401 ctacacagtt ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn ntcggcgcaa gccatttttg gccggtgcac tggttgggag gcatgctgtt
     2581 cgccgcagtc ctgatcgcag tagcggcgct gttgttgcgg ctcgtttcgt tgggggagat
     2641 gagggggctg atgaagcgct aatttttccc ctcgcgctcc gtccaatatt ttttaccttt
     2701 gccgttcatt tttacagacc atacaaaacc tggtagtaca tgaatccttt caggatatta
     2761 tccaccttga ccggcttggt cgtttttgcc atctccggcc tcgtgtattt caattccgta
     2821 gaatccaccg gcagcttgtg ggactgcggc gagttcatcc tcggcgccta cannnnnnnn
     2881 nnnnnnnnnn nnnngtagtt cacccgcccg gagcgccggt attcctgctc atcggtcgca
     2941 tgttcacctg ggtggcagag atgatctccg acgatcccgc cgatattgcc gtctctatca
     3001 acctgctttc ggccctgtgc agcgctgccg ccgccatgct cgtagcttgg acggccatga
     3061 tcttcggcaa gttctccctc actggcagcc gcgaagcgga aaccaatctg ggccaaaaca
     3121 tcgcgctggt tgcagccggc ttagccgccg gtttggctac ggccttctcc acttccatct
     3181 ggttttcagc agtggaaggc gaagtatatg ccatgtccac cttcttcacg gcgctcacgc
     3241 tgtgggccat ggtgaagtgg taccacctgc ccgacacccc gcagtccgac cgttgggtgt
     3301 tgttcgccgt gtattccgcg ggcttgtcta tgggcgtaca cctgctcagc ttgctcacgt
     3361 ttccggcatt ggccatgctg tattatttca aaaaatacaa agaacacacc ttcatgggca
     3421 tggcgctggc cgcattggtc ggggtggtgt tcatcgtagg cgtgcagtct tttgtcattg
     3481 taggcattcc gagtctttgg ggcgcttttg atatcatgat ggtcaatggt ctgggcatgc
     3541 ccttccattc gggcctcgtt cctaccatac tcatcatcgg cggctacatt gcgttgatga
     3601 cgctcatttt gcaggaaaaa atcacctcgc gtgcgccgat gttcgtcttt tcgggggtta
     3661 tgtttttatt ttcggcgata tgcacaatgg gcttcaccga atatgaaggc gtcaagtttt
     3721 tgtggttggg caccctgctt acattgataa cggcttttgc tcaggacgcc ctcgttcagc
     3781 gccgccgcga cgcgcagatg atcctcatgg gcgctacctt ggtcatcgta tcgttctcta
     3841 ccattggcgt tgtcgtcatt cgggccaatg cccagacgcc cgtcaacatg aacgcgccta
     3901 ccgatgccac ccgcctcatc ccgtacctca accgcgaaca atacggcgag cgcgcattgg
     3961 tgaaaggccc gcattttatg gcctctcccg acggcaacaa gtcgaaatac aaaccgcgtt
     4021 tcggcccggt aggtgatcgt tatgaactga ttgacgaaaa aatcacttac ggtttcaagt
     4081 cttccgacga aatgctcttc cccagaatcg gcgacttcct gagcgacgac cgcaaagggc
     4141 tgtacaaagc ttggctggga cttgatcccg aaaaaccgct gcctcccggt cgccccaatc
     4201 aggccgacaa catcagcttc ttatggaact accagatcaa ctggatgtac tggcgctatt
     4261 tcatgtggaa tttcgccggc aaacaaaatg atgtacaggg catacatatg gggaatgtgc
     4321 gcgatggaaa ctggatcacc ggcattgggt tttacgacaa ccttcgcctg ggcgaccaga
     4381 aatacatgcc cgacagcctg aaacataaca aagccaacaa taaattattc gcactgccct
     4441 ttatcctggg cttgctcgga ttgttttacc aggccagaaa agataagaag gacgcgctta
     4501 ttacaggctt gctgttcttc tttacgggta ttg
//
LOCUS       DAORLF010000404         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10482_length_4518_cov_1.974681, whole genome shotgun sequence.
ACCESSION   DAORLF010000404 DAORLF010000000
VERSION     DAORLF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10482_length_4518_cov_1.974681"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1162
                     /locus_tag="PK198_22565"
     CDS             200..1162
                     /locus_tag="PK198_22565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF41602.1"
                     /translation="MPPQVSFIIPLYNEEQVFGKLEARIRALAPQLPFSAEFILVDDG
                     SRDATPLLMRRLATQDPRFKAVFLARNFGHQIAVSAGMDYVAATEAVMILDGDLQDPP
                     ELATQFYEHIRQGYEVVYAIRKKRKENALKRSAYWSFYRLLNAISTIDIPLDSGDFCM
                     ISRRVNDWIRSMPERSRFIRGMRTWVGFRQIGLEYEREARQAGEAKYNFKMMFRLAYD
                     GIFNFSEVPLRLITKLGVATITLSLVYLVATLAFRSMGGEVPRGFITTIFAITLFSGV
                     QLVSLGIIGEYLSRIYTQVKERPLFIVREVIEDGKEKIITLENK"
     gene            1166..>1835
                     /locus_tag="PK198_22570"
     CDS             1166..>1835
                     /locus_tag="PK198_22570"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF41603.1"
                     /translation="MEKPRHPHDHFFRQTFGIPEIAKDYLRNFLPTQLAQQIQLDSIE
                     RVEGAFVSNKLRAHFSDIVYRCVLKDGGNAWLTFLFEHKSKQAKYPHIQLLRYMLEIW
                     ERAVKEKKRLPLIIPIIVYHGKKRWNLKPFESYFEGWSVTLRPYLPAFDYLLTDLSGL
                     SDEEIINIEARFLINTFLSLKHQGEKDYLRKHIATVFYQLNDSEYDEYEKNYIQMLLV
                     YLLCT"
     assembly_gap    1836..1849
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1850..2132
                     /locus_tag="PK198_22575"
     CDS             <1850..2132
                     /locus_tag="PK198_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41604.1"
                     /translation="NDAEWERMINDIPSSVKPLAMSTYDLLIKKGIEQGIERGIERGI
                     ERGIEMHRRESVINLLTLTQLTINEIAIITATSVSYVTELQKHLHNQDN"
     assembly_gap    2218..2233
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2234..2448
                     /locus_tag="PK198_22580"
     CDS             <2234..2448
                     /locus_tag="PK198_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41605.1"
                     /translation="GIEQGIQQGAEIFRLEAIPKLLKLNKLTIEEIAFITASPVSYVK
                     DLHNKLIADGERVSDYLKNTTTSSAN"
     gene            2489..3688
                     /locus_tag="PK198_22585"
     CDS             2489..3688
                     /locus_tag="PK198_22585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRF41606.1"
                     /translation="MPIPFCLPLIDRDVVAEVNDCLTNTGWLTTGPKVRELETEIRKL
                     SGTPVLCVNSWTSGAMLMLRWFGIGPGDEVVIPAYTYSATALCALNIGASVVMVDVGS
                     DFNIDAEKLRRAITPRTKAVIPVDIGGWLCNYDALHAVLNSTEVRSRFQPANARQEQL
                     GRPLLLADAAHSLGARYQNKPSGALADATVFSLHSVKNVTTGEGGAICLALPHPFDNE
                     AEYLFLRAFALNGQTKSAFEKNQPGAWRYDIIDQGLKINMPDICAAIGLAQIRKYTSE
                     LLPNRHRIFQQYNHAFASFDWAILPPYRDEERASSCHLYMLRIAGANEEARDEIIRRI
                     SEKGVGVNVHYIPMAMLTLFKNLGYRMEDYPETYRLYANEITLPVYNGLTEAQVDEVA
                     KAVVASV"
     gene            3741..4418
                     /locus_tag="PK198_22590"
     CDS             3741..4418
                     /locus_tag="PK198_22590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRF41607.1"
                     /translation="MYPFIKRLFDLLFATVGLLLLSPLLLPIIFLLRITGEGEVFYRQ
                     KRVGFKNGHFGIWKFATMLKNSPNMGTGSLTLRNDPRVTPMGGFLRKTKINELPQLIN
                     VLTGEMSFVGPRPQMQVDFDVYPPEVRARIYDVTPGITGIGSIVFRDEERLLSRPGIE
                     PKVFYAQHIAPYKGALEMWYLRRRSFYTDTMILLLTAWAIVSSESQLHFRAFHDLPPL
                     PAELLTP"
BASE COUNT         1124 a         1427 c          956 g          981 t
ORIGIN      
        1 cttcgcgccc ttcgctataa ttcgcgttaa aaaaatccac tctacgttcg cggaacttcg
       61 cgcccttcgc tataattcgc gttaaaaaaa tccactctac gttcgcggaa cttcgcgccc
      121 ttcgctataa ttcgcgttaa aaaaatccac tctacgttcg cggaatttcg cgccgcttcg
      181 ccattattcg cgttagaaaa tgccaccaca agtctccttc atcataccgc tctacaacga
      241 agagcaagta ttcggcaagc tcgaagcacg catccgcgcc ctcgcgccac aactcccctt
      301 cagcgccgag ttcatcctcg ttgacgacgg cagccgcgac gccacccctc tcctcatgcg
      361 ccgcttagcc acccaagacc cgcgcttcaa agccgtgttt ctggcccgca atttcggcca
      421 ccagatcgcc gtcagcgccg gcatggacta cgtcgccgcc accgaggccg tcatgatcct
      481 cgacggcgac ctacaggacc cacccgaact cgccacacag ttttacgaac acatccgcca
      541 ggggtacgaa gtcgtttacg ccatccgcaa aaaacgcaaa gaaaacgcgc tcaaacgctc
      601 cgcctactgg agtttttacc gcttgctcaa cgccattagc accatagaca tccccttgga
      661 cagcggcgat ttttgcatga tttcccgccg cgtcaacgac tggatacgct ccatgcccga
      721 gcgcagccgc ttcatccggg gcatgcgcac ctgggtcggc ttccgccaaa tcggattaga
      781 atacgagcga gaggcccggc aggccggcga ggccaaatac aatttcaaaa tgatgttccg
      841 cttagcttac gacggcattt tcaatttcag tgaagtcccc ctccggctca tcaccaaact
      901 cggcgttgcc accatcactc tctccttggt ttatctcgtt gccaccctcg ctttccgcag
      961 catgggcggc gaggtaccgc gcggcttcat caccaccatc tttgccatca ccctgttcag
     1021 cggcgtgcaa ttggtcagcc tcggcatcat cggcgagtac ctctcgcgca tttacacaca
     1081 agtcaaagag cgcccgctgt ttattgtgag agaggtcatt gaagatggta aagagaaaat
     1141 aataaccctt gaaaataaat aacttatgga aaaaccccgg cacccgcacg atcacttttt
     1201 tcgccaaacc ttcggcattc cggaaatagc aaaggactat ttgcggaatt ttctccccac
     1261 gcaattagct caacaaatac aattggatag catagaaagg gtcgaaggcg cctttgtgag
     1321 caacaaattg cgcgcccatt tttccgacat tgtttatcgc tgtgtattga aagatggagg
     1381 aaacgcctgg ctgacttttc tttttgaaca taaaagcaaa caggcgaagt acccgcatat
     1441 ccaactgctg cgatacatgc tcgaaatatg ggagcgagcc gtcaaagaaa agaaaaggct
     1501 gcctctcatc atccccatca tcgtgtatca cggaaaaaaa cggtggaacc tgaaaccttt
     1561 tgaatcttat tttgaaggtt ggagtgtaac gctccggccc tatctgccgg ctttcgatta
     1621 tttactgacc gatttgtcag gattaagcga tgaggagatc atcaatattg aggccaggtt
     1681 cctgatcaat acttttttat cactcaaaca tcaaggagaa aaagactacc tgcgcaaaca
     1741 cattgcgacc gttttttatc aactgaatga cagcgaatac gacgaatacg aaaaaaacta
     1801 tatccagatg ttgcttgttt acctcctatg tacttnnnnn nnnnnnnnnc aatgatgctg
     1861 aatgggagcg tatgataaat gatattccgt caagtgtaaa acctttagcc atgagtactt
     1921 atgatcttct gattaagaaa ggcattgagc aaggcatcga gcgaggcatt gagcgaggca
     1981 ttgagcgagg catcgagatg catcgaaggg aatcagttat caatctcctg actctaaccc
     2041 aattgacgat aaacgaaata gccatcataa ccgccacctc cgtttcttat gtgacagaat
     2101 tgcaaaaaca cttgcacaat caagacaatt aaatcgctca agaccttctg aaggtaaacg
     2161 cccaaaccta aatattaaaa acctgcaacc atgagtacct ttgatcatat tgtacaannn
     2221 nnnnnnnnnn nnnaaggtat agaacaagga atacagcaag gcgccgagat attccgtctg
     2281 gaggccattc ccaagctatt gaaactgaat aagctaacca tcgaagaaat tgccttcata
     2341 acagccagtc ctgtttctta tgtgaaagac ctgcacaata aactaattgc cgatggagaa
     2401 cgtgtgtccg actacttaaa aaatacgacc acgtcatcgg ctaattaatc aaaaagggcc
     2461 actcgcccct caccgctcac gcttcccaat gcccatccct ttctgcctcc ccctcattga
     2521 ccgcgacgtc gtcgccgaag tcaatgactg tctcaccaac accggctggc tcaccacagg
     2581 cccgaaagtc cgcgaattgg aaaccgagat tcgcaagctc agcggcacgc ccgtcctctg
     2641 cgtcaactca tggacctccg gcgccatgct catgctccgc tggttcggca tcggccccgg
     2701 cgacgaggtc gtcatcccgg cctacaccta cagcgccaca gccttgtgcg ccctcaatat
     2761 cggcgccagt gtagtgatgg tggacgtcgg ttccgatttc aatatagacg ccgaaaagct
     2821 ccgccgcgcc atcacgcctc gcaccaaagc cgtcatcccg gtggacatcg gcggctggct
     2881 atgcaactac gacgccctcc atgccgtact caactcgacg gaagttcgca gccgcttcca
     2941 acccgcgaat gcccgtcaag agcaattggg ccgcccgctt ttgctggccg acgccgccca
     3001 ctcgctcggt gcaagatatc aaaacaaacc ctccggcgcc ttagccgatg caacggtatt
     3061 ctccctgcac tccgtcaaaa acgtcactac cggagagggc ggcgccatct gcctcgcact
     3121 cccccaccct ttcgacaacg aagccgaata cctcttcctc cgcgctttcg ccctcaacgg
     3181 ccaaaccaaa agcgcttttg aaaaaaacca gcccggcgcc tggcgctacg acatcataga
     3241 tcagggcctc aaaatcaata tgcccgacat ttgcgccgcc atcggcctgg cccaaatcag
     3301 aaaatatacc tccgaactgc tgcccaaccg acatcgcatt tttcaacaat acaaccatgc
     3361 attcgcctcc ttcgattggg ccatcctgcc gccttaccgc gacgaagagc gcgcctcctc
     3421 ctgccacctc tacatgctcc gcatcgccgg cgccaacgag gaagcccgcg atgaaatcat
     3481 ccgccgtatc tccgaaaaag gcgtgggcgt caatgtccat tacatcccca tggccatgct
     3541 caccctcttc aaaaatctcg gctaccgcat ggaagactat cccgaaacct accggctcta
     3601 cgccaacgaa atcacccttc ccgtttacaa cggcctcacc gaagcgcagg tggacgaggt
     3661 agcaaaggcc gtcgttgctt cggtgtgatt acctcaaatc acccactcac acactcaccc
     3721 ctcgcccact caccactgcc atgtacccgt tcatcaaacg cctcttcgat ctcctcttcg
     3781 ccactgtcgg cctcctcctg ctctcgccgc tgttgttgcc cattatattt ttgctgcgca
     3841 tcaccgggga gggcgaggta ttttaccgcc aaaaaagagt aggctttaaa aatgggcatt
     3901 tcggcatctg gaaatttgcc accatgctca aaaacagtcc caacatgggc accggcagcc
     3961 tcaccctccg caacgacccc cgcgtgacgc ccatgggcgg gttcctgcgc aaaaccaaaa
     4021 tcaacgaact gccgcaactc atcaacgtcc tcaccggaga gatgagcttc gtaggcccgc
     4081 gcccgcaaat gcaggttgac ttcgacgtgt atccccccga agtgcgcgcc cgtatttacg
     4141 acgtcacgcc cggcattacc ggcatcggtt ccatcgtttt ccgcgacgaa gaacgcctgc
     4201 tctcccggcc cggcatcgaa cccaaagtct tttatgcaca gcacattgcc ccctacaaag
     4261 gcgcgttgga aatgtggtac ctgcgccgcc gttcctttta taccgatacc atgattttgc
     4321 tgctcaccgc ctgggccatt gtttcatccg aatcacaact gcattttcgc gctttccacg
     4381 atttgccccc tttgcccgcg gagcttctca cgccatgaca ctccttcaaa acatccaaca
     4441 cctcgccggc aatgcccaat ccgagctgat tcgcctgcgc cgtcacctgc acgcctggcc
     4501 cgaattgtcg tttcagga
//
LOCUS       DAORLF010000405         4493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10554_length_4493_cov_2.260703, whole genome shotgun sequence.
ACCESSION   DAORLF010000405 DAORLF010000000
VERSION     DAORLF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_4493_cov_2.260703"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>455
                     /locus_tag="PK198_22595"
     CDS             219..>455
                     /locus_tag="PK198_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41608.1"
                     /translation="MTHTDQQHINSIRTGDNAGLHRIYREFLPRIHALITQNGGSYDD
                     ARDVFQDALVVLFDKCRTENFALSSSFSTLLYGVC"
     assembly_gap    456..479
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <480..804
                     /locus_tag="PK198_22600"
     CDS             <480..804
                     /locus_tag="PK198_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41609.1"
                     /translation="LQKKSRSEVSLTDDLKFTDSTDIEFDLVQAERESIFWDALRRLG
                     TECRKLLELFFEKKSMEEIAETMQLSSVGYAKKRKFICKEQLIEQVKSDVRYKDWDGD
                     ASREF"
     gene            839..1666
                     /locus_tag="PK198_22605"
     CDS             839..1666
                     /locus_tag="PK198_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41610.1"
                     /translation="MKEQLYERIEAYIAGALSEEERARLEQEIAADPALAEEVALHRS
                     LDQLMADKGKMNFRRQLDEVGQAYAQPPASGLRWFWPVVLLAGALAALWWFILRPQPE
                     PTPAPIPEAPTVQDTIADVPPPQDTAPLAPPPKPEKPAPRDLFAPVPALEKAIQKEPD
                     AYFTIETALLDALPGADARSRQIRFSGRLLTAMDLPKLELQLLDNTRPDGKLVVRIPV
                     TATLVEEDENIRAFAAKKLYLLDAAQNLLLPNGLYYAQLVVVADNKVLWTGKVGGGR"
     gene            1821..4025
                     /locus_tag="PK198_22610"
     CDS             1821..4025
                     /locus_tag="PK198_22610"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRF41611.1"
                     /translation="MMTKRFLIAISILAGLLPGRLHGQLKPLIPPQGTTRAVIVGVSE
                     YSNERIPRLQFAHADADAFARFLRSEAGGAVPENQIKLLTNRQATQGQMAAALTWLLE
                     ESKEGDMALIFFSGHGDMETQTVMNHGFLLTHDAPASTYMAGGAFPVFYLQSIVQTLS
                     TQRKAQVALITDACHAGKLAGSANRGAQATAQLLAEQFANEARLLSCQPDEFSAEGAQ
                     WGGGHGVFTYFLLDGLTGLADANQDQTVTLLELQRFLEDQVPAAVAPASQIPLVSGNK
                     GMKMARVNEGVLAALKERRAVVSAHISNTVAFRSVAVYNPEDSTVQAMYSRFQSALSQ
                     KRLLPPAEDDAYSLYITLRKQPAAVSLHNEMRRSLAAALMDEAQQAINDYLAANPLEL
                     RRRWSFDARYEQFPTYLQKAAEVLGETHFAYRDLQTKALYFNGLNLRLRGERERNRDL
                     YRQADSVQQQVLASDPNAAYAYNELGLLARRMRSFETAATHFQQALALSPTWVLAETN
                     LCATLVDLMRPAEAEASCRRALQYDSTFALAHHNLGAALAEQQQWDAAIASLQKALSF
                     DDQYYLTHWKLGDVYYAADNKEQALNAWKGCIALEPTYIPALFNAGIVCRELMRPEEA
                     LSYFQQVAATDPNDVGAWVEIAELHTDAGLLDQADTALQEAFRLSPDSPDVYYAAARL
                     HCAQKSFTRALSELAIAVGLGFDNKQRFLSDAIMSPLYKKKEVKRWLKACKN"
     gene            complement(4123..>4493)
                     /locus_tag="PK198_22615"
     CDS             complement(4123..>4493)
                     /locus_tag="PK198_22615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41612.1"
                     /translation="VNTVTTAAFNNVSVTGGGALIAPVTTAQAIDAPAMPEVAVFPNP
                     SNGTLNIDLAQYAGREVRLELFSAQGQLLKTVEIDEVQTQIEQMNLSAFENGMYMLRV
                     QSEGLPEVSRRVVLSKAGKQ"
BASE COUNT         1033 a         1336 c         1154 g          946 t
ORIGIN      
        1 tgaagatgag ttgatagcag ggactcctta tagtgtacat cgctaccggc gattgttttt
       61 ttgatttttt gtactgaaat agaagactct acaccttccg ggctgcgctt ttgtactacc
      121 ttacatttgc cccaaatctc aaaaagttat cccccggcag gtaaaagtac ttttttccac
      181 aaaaatcagc ttacctttcg ccagattttc gcaagtccat gacccatacc gatcaacaac
      241 atatcaattc catccgcacc ggcgacaacg caggcctgca ccgcatctac cgcgagttcc
      301 tgccccgcat ccatgcgctc atcacacaaa acggcggcag ctacgacgat gcccgcgatg
      361 tttttcagga tgcgctcgtc gtgctcttcg acaaatgccg gacggaaaat tttgccctca
      421 gcagctcttt ttccaccctg ctctacggcg tgtgtnnnnn nnnnnnnnnn nnnnnnnnnc
      481 ctccagaaaa aatcccgctc cgaggtatcc cttaccgatg atctcaaatt cacagacagc
      541 accgacattg agttcgatct cgtgcaggcc gaacgggaaa gcattttctg ggatgccctg
      601 cgccggctcg gaaccgaatg ccggaagctg ctggaactgt tttttgagaa aaaaagcatg
      661 gaggaaattg ctgaaaccat gcagctctcc agcgtgggat atgccaaaaa acgaaaattt
      721 atatgcaagg aacaactcat cgaacaggtc aaatcggatg tccgatacaa ggactgggac
      781 ggggacgcga gccgcgagtt ctgagtgaga ttcagcgctc agctatatga aataaacaat
      841 gaaagaacaa ctgtacgaac gcatcgaggc ttacatagcc ggcgcccttt ccgaggagga
      901 acgcgcccgc ttagagcagg aaatagctgc cgatccggct ctggccgaag aggtggccct
      961 gcaccgcagc ctcgaccaac tgatggccga caagggaaaa atgaacttcc gccgccaatt
     1021 ggacgaagtc ggacaggctt atgcacagcc gcccgcttcc ggcttgcgtt ggttttggcc
     1081 tgttgtgctg ctggccggcg ctttggcagc actctggtgg ttcattcttc gccctcaacc
     1141 ggaaccgacg ccggccccca ttcccgaagc gccgactgta caggatacca tcgccgatgt
     1201 gccgccgccg caggataccg ctcctctcgc accgccgccc aagcccgaaa agcctgcccc
     1261 gcgcgatctt ttcgcgcccg tgcccgccct cgaaaaggcc attcaaaagg aacctgatgc
     1321 ttacttcacc atagaaaccg ccctgctgga cgccctgccc ggagcagatg cccgcagccg
     1381 gcaaatccgc ttctccggca ggctgctcac agctatggac ctgccgaaat tggaactaca
     1441 acttcttgac aatacgaggc ccgacgggaa gctcgtcgtt cgcataccag tgaccgccac
     1501 cttggtagaa gaagatgaaa acatccgggc ttttgccgcc aaaaagctct acctgttgga
     1561 cgccgcgcaa aacctgctgt tgcccaatgg gttgtattac gcgcagttgg tcgtcgtggc
     1621 cgacaataaa gtcttatgga cggggaaggt gggcggtggg cggtaggcgg tgctaagtgc
     1681 gagggaaccc gccttcccac gcctgtagcg tggttcggcg gatgaaaccc ggaaccttga
     1741 acccggaacc cgaaaccggt aacgttttcc cattctgaca aatcaactat tcaattatac
     1801 gcacccaaac cggcataccc atgatgacca aacgtttttt gatcgccatt tccatcctcg
     1861 ccggcttgct gcccgggcgc ctgcatggcc aactcaagcc cctgataccg ccgcagggta
     1921 ctacccgcgc cgtcattgtg ggcgtgtccg aatacagcaa cgagcgcata ccccgcctac
     1981 agtttgcaca cgccgatgcc gacgctttcg cccgtttcct gcgctcggag gccggcggcg
     2041 ctgttcctga aaatcagatc aaactgctga ccaaccggca ggccacgcaa ggtcaaatgg
     2101 cggccgccct cacctggctg ctcgaagaaa gcaaagaggg cgacatggcg ctcatttttt
     2161 tctccggcca cggcgatatg gaaacgcaaa ccgttatgaa tcatggcttt ctgcttaccc
     2221 acgacgcgcc ggcctccacc tacatggcgg gcggagcctt cccggttttt tatctgcaat
     2281 ccattgtaca aaccctcagc acccaacgca aagcgcaggt ggcgctcatc accgatgcct
     2341 gccacgccgg aaaactcgcc ggcagcgcca accggggcgc acaggctaca gctcaattgc
     2401 tggcagaaca gtttgccaac gaagcccggc tgctctcctg ccaacccgac gagttctcgg
     2461 cagagggcgc gcagtggggc ggcgggcatg gcgtattcac ctattttcta ttggacggac
     2521 tgaccggact ggccgacgcc aaccaggatc agacggtgac gctgctggaa ttgcaacgtt
     2581 ttttggaaga tcaggtgcct gcagccgtag cgccggccag ccagattccg ctggtctccg
     2641 gcaataaagg catgaaaatg gcccgtgtaa atgagggcgt gctggccgcg ctcaaagaac
     2701 gcagggcagt cgtttccgcc catatttcca atacagtagc cttccgttct gttgccgttt
     2761 acaaccccga agactctacc gtgcaggcca tgtacagtcg ttttcagtcg gctctgtcac
     2821 aaaaacggct cttgccgccc gccgaagacg atgcttacag cctgtacata actttgcgaa
     2881 agcagcctgc cgcagtctct ttgcacaatg aaatgcgccg cagtctggcc gccgcgctga
     2941 tggacgaggc acagcaagcc atcaacgatt atctggccgc caatccatta gaactgaggc
     3001 gccgttggtc gttcgatgcc cggtacgagc agtttccaac gtatttgcaa aaagcggcgg
     3061 aagtattggg cgaaacccat tttgcctacc gggatttaca aaccaaagca ctgtatttca
     3121 atggcttaaa tttgcgtctc cgaggcgaac gcgagcgcaa tcgggacttg taccggcagg
     3181 ccgattccgt tcaacagcaa gtgctggcct ccgatccgaa cgcagcttat gcctacaacg
     3241 aactggggtt gctggcccgc cgcatgagga gttttgaaac cgccgccaca catttccagc
     3301 aagcactggc cctgtcgccc acctgggtgc tggccgaaac caacctctgt gcaacccttg
     3361 tggacctgat gcgccccgcc gaagccgagg cttcctgccg gcgggcattg caatacgaca
     3421 gcacctttgc actggcgcac cataatctgg gcgctgcact ggccgaacaa caacagtggg
     3481 acgcggcaat agcatccctg caaaaggccc tgtccttcga cgatcaatac tacctgacac
     3541 actggaaatt gggcgatgtt tattacgctg cggacaacaa agagcaggcg ctcaacgcat
     3601 ggaaagggtg tatcgccttg gaaccgacct acattccggc attgttcaac gcgggcatcg
     3661 tttgccgcga actcatgcgc cccgaagaag ctctttccta ttttcaacaa gtggcagcca
     3721 cagaccccaa cgatgtcggc gcttgggtgg aaattgccga gttgcacacc gatgcgggcc
     3781 tcctcgacca ggcagatact gccttgcagg aggcatttcg gctttcgccg gattcgcccg
     3841 atgtgtacta cgccgccgcc cgattgcatt gtgcgcagaa aagtttcacg cgcgccctgt
     3901 ctgagttagc aattgctgtg ggtctgggat ttgacaacaa acaacgcttt ttgagcgatg
     3961 ccatcatgtc tccgctgtac aaaaagaagg aagtgaagcg ctggctaaaa gcgtgcaaaa
     4021 attgaagcat tccaacgccg attaaaataa aaagagccac cccgattgct caggatggct
     4081 cttcatgatt cttgaaaaga gatcaggaaa atcctggaaa tcttattgtt tgcctgcttt
     4141 gctcaatacc acgcgccggc tgacttcggg caagccttcg ctttgtacgc gcagcatgta
     4201 catgccgttt tcaaaagcag agagattcat ttgctctatc tgcgtttgta cctcgtcaat
     4261 ttcaaccgtt ttgagcaact gcccctgcgc gctgaacaat tccagacgaa cctccctgcc
     4321 tgcgtactga gccaggtcta tgttcaaggt tccgttgctc gggttgggga agacggctac
     4381 ttccggcata gcgggagcat ctattgcctg agcggtagtt accggagcta tcaatgcgcc
     4441 gccgcccgta acggatacgt tgttgaatgc agcggttgtc accgtattca cgt
//
LOCUS       DAORLF010000406         4457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10673_length_4457_cov_2.017492, whole genome shotgun sequence.
ACCESSION   DAORLF010000406 DAORLF010000000
VERSION     DAORLF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_4457_cov_2.017492"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    98..286
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(446..1219)
                     /locus_tag="PK198_22620"
     CDS             complement(446..1219)
                     /locus_tag="PK198_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRF41613.1"
                     /translation="MAVGIISYNLNGIRSAASKGLEDWIRAGQYDILCFQETKAEKDQ
                     FDDSALEAMGYHGYWHSAEKKGYSGVAIYTKIKPDQVIAGCGMEKYDREGRILRADFG
                     DWTLLNCYFPSGTTGDERQAFKMEFLEDFFQWVQELRRERPQLVVVGDYNIAHHPIDI
                     HDPVGNKKSSGFLPEERAWMTRWFESGFTDAFRHLHPEAVSYSWWSFRAGSRARNKGW
                     RIDYQSVTDNLRGQIRQAAQLPDAVHSDHCPVWVELEVH"
     gene            complement(1219..1656)
                     /locus_tag="PK198_22625"
     CDS             complement(1219..1656)
                     /locus_tag="PK198_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41614.1"
                     /translation="MARVYEAYADYSDADFPFRGCDQLSTWPEVATLAAIAQDLGVNT
                     SDNAAAAAQRARLFSAPAAPQKENEISLENWNIRLWKGLDAWAQRDPLNKEMVTAFRA
                     AYHQSQVRLEDHFMDKGFNAEAARSLALQTLQTLTAHHLQRFL"
     gene            complement(1640..>2271)
                     /locus_tag="PK198_22630"
     CDS             complement(1640..>2271)
                     /locus_tag="PK198_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41615.1"
                     /translation="NTPQQPASPQPATPTMQPRSTGAIALVQAPVIRSNVKRRSVLDL
                     QQVLKEQGAYTGSLDGYYGNGTANAYNDFAGSHRQTMRYAASTEHNTRPGMTPTAIRF
                     QRALDAMNDDAAAVVEVTTANGPLAKAWQAYVLYTNRGASADVNQLMNTAIRESFDGK
                     ILRKSPPFDTRSTYSYTSLHPLPAQRARQLSDHALLAGPSPSRRDGPCV"
     assembly_gap    2272..2294
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2295..2954)
                     /locus_tag="PK198_22635"
     CDS             complement(<2295..2954)
                     /locus_tag="PK198_22635"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF41616.1"
                     /translation="MLKRTLFMAVALFTAASFLSAQERYTVQIGNFMEARADDFSKLR
                     SIGFVHAAPVDAGVSRIMLGGYDAKAAADRAVAAVRAAGYPNAYAMQVFLNEGMNVAI
                     IQLGAEKSNAKTNWARYAAAGDLYGIMGVDDVKIVAGPYANVEEARKKLDDIKKAGFR
                     DAFVKVVNSMTLHKLTEFETGIKKPLIPLQIQPVKPAEPASSISVIPPSYEQTQSRIK
                     SP"
     gene            complement(3333..>4457)
                     /locus_tag="PK198_22640"
     CDS             complement(3333..>4457)
                     /locus_tag="PK198_22640"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HRF41617.1"
                     /translation="GHNLMQAIARVNRVSKDKPGGLIVDYLGIASDLKNALAFYADAG
                     GKGDPTILQEQAVALMLEKLEVVSQMFHGFEYETYFDAETSKKLSMILAAEEHSLGLE
                     DGKKRFINEVTALSKSFALAIPHEQAMDVKDEVSFFQAVKARLAKFDITGSGRTDEEI
                     ETTIRQVIDKALVSEQVIDVFDAAGIKKPDISILSEEFLLELKGMEHKNVALEVLKKL
                     LNDEIKSRAKQNLVKSRTFLEMLENSIKKYHNKILSAAEVIEELIKLSKDIVEMDNEA
                     KTMGLTDFEYAFYTAVANNDSARDLMQKDQLRELAVALTETIRQNASIDWTIKESVKA
                     KLKVAVKRLLRKYGYPPDMQMLATETVLKQAEMIANEMMA"
BASE COUNT          930 a         1093 c         1120 g         1102 t
ORIGIN      
        1 tcgtggaaag atggccggta tcgttctttt tttgagttcg tttccacttg cgtcgaatgg
       61 cctttgtttg tctaatggat tgtttgtcaa aggctatnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaca atccctttta
      301 ttttacgaaa atcgctcgcc gacagttcga ttgccgaaat caagatcact aaagctgata
      361 tgatgaatct ggcacttatc atcttttttt gtttcaatcc caagaaatcc tgctaagctc
      421 atggcttgcg gcttgcagcc tgatactaat ggacctccaa ctccacccac accgggcaat
      481 gatcggaatg taccgcatcg ggcaactgag ccgcctgccg gatttgaccc cgaaggttgt
      541 cggtaacaga ctggtaatca atacgccaac ctttgtttct ggcccgcgaa ccagccctga
      601 agctccacca actgtacgac acggcctccg ggtgcaaatg ccggaaagca tccgtgaagc
      661 cgctctcgaa ccagcgcgtc atccatgcgc gttcttcggg caggaagccg gaagattttt
      721 tgttgcccac cggatcgtgg atgtctatgg ggtggtgcgc gatgttgtag tctcctacca
      781 ccaccaattg ggggcgctca cggcgcaatt cctgcaccca ctggaaaaaa tcttccagaa
      841 attccatttt gaaagcctgc cgttcatcac cagtagtgcc ggacgggaag tagcaattga
      901 gcagcgtcca gtcgccaaaa tccgcgcgga ggatgcgccc ttcgcggtcg tatttttcca
      961 tgccgcagcc ggcaatcact tggtcgggct tgattttggt gtaaatggcc acgccgctgt
     1021 aacccttctt ctcggcgctg tgccaatagc cgtgatagcc cattgcctcc agagcggagt
     1081 cgtcaaactg gtctttctcc gctttggttt cctgaaaaca gaggatgtcg tattggccgg
     1141 cgcgaatcca gtcttccagc cctttggacg cagccgagcg aatgccgttg agattgtaag
     1201 aaatgatgcc gacagccatc agaggaagcg ttggaggtgg tgagcagtga gggtttgcag
     1261 cgtttgcagg gccagcgaac gggcggcctc cgcattgaaa cccttgtcca taaaatgatc
     1321 ttcgaggcgt acctgcgact gatggtaagc ggcgcggaat gcagtgacca tttctttgtt
     1381 cagcggatcg cgctgcgccc aggcatctaa gcccttccac aggcggatgt tccagttttc
     1441 cagactgatt tcgttttctt tctgcggggc agcgggcgca ctgaacaggc gggcgcgttg
     1501 tgcggcagcg gcggcattgt cgctcgtgtt cacccccagg tcttgcgcga tggccgcgag
     1561 ggtagccact tcgggccagg tagagagctg atcgcagccg cggaagggga agtcggcgtc
     1621 ggaataatcg gcgtaggctt catacacacg ggccatctcg gcgggatggc gaggggccag
     1681 ccagcagggc gtggtcacta agttgccggg cgcgctgtgc aggaagtgga tgtaaagacg
     1741 tgtaggaata ggtagagcgc gtgtcgaaag gcggcgattt tcggagaatt tttccgtcga
     1801 aggactccct gatggctgtg ttcatcaatt gattgacatc tgcggaagcg ccccggttgg
     1861 tgtacaacac atacgcctgc caggctttgg ctaaggggcc gttggcagta gtcacttcca
     1921 caacggcggc ggcatcgtcg ttcatggcat ccaatgcgcg ctggaacctg atggctgtgg
     1981 gcgtcatgcc cggccgggtg ttatgctcag tcgaggcggc ataccgcatg gtctggcggt
     2041 ggctgccggc aaaatcgttg taagcattgg ctgtgccgtt gccataataa ccgtctaaag
     2101 aaccggtgta agcgccctgt tctttgagta cctgttgcaa atccaatacc gagcggcgct
     2161 tcacattact gcgaataacg ggcgcctgca ccaatgcaat ggcccccgtg gagcgcggct
     2221 gcatggtggg cgttgcgggc tgtggcgacg cgggttgctg cggggtgttt annnnnnnnn
     2281 nnnnnnnnnn nnnncgggct tttgatgcgc gactgtgttt gctcgtaaga aggtggaatg
     2341 acgctgatgg aggaggcggg ttcagcgggc tttaccggtt gtatttgcag cggaatgagc
     2401 ggttttttga tgcccgtttc aaactcggtc aatttgtgca gggtcatgct gttgaccact
     2461 tttacaaagg cgtcgcggaa gccggctttt ttgatatcgt ccaatttttt gcgcgcttct
     2521 tccacattgg cataaggtcc tgccacgatt ttcacgtcgt ccactcccat gataccgtac
     2581 aaatcgccgg cggcggcata gcgcgcccag ttagttttgg cgttcgactt ctcggcgccc
     2641 aactgtatga tggccacatt catgccctca ttgagaaaaa cctgcatggc atacgcattg
     2701 gggtacccgg cagctcgcac ggcagctacg gcgcggtcgg cagcggcttt ggcatcgtat
     2761 ccgcccagca tgatgcgcga cacgccggcg tccacaggcg cggcatgcac aaagccgatg
     2821 cttctgagtt tggaaaaatc atcggcgcgg gcctccataa aattgccgat ttgtacggtg
     2881 tagcgctctt gcgccgagag gaaagaggcc gcagtgaaca gtgcaaccgc catgaaaaga
     2941 gttcgtttca gcattcgttt cagattaagt actgatctaa atcatcgcgt tgataaacgc
     3001 aaacgcatgg ccaaaagtac ctctttttgt agaaaatcaa aaactcggct gcgggagtgt
     3061 atattccgac gcatctgccc cccctgagtg ttgataactt tccggaaggt ctgacccccc
     3121 ctgaaaatgt ggatagatgt gtaacttcat caacggatca agatggtaaa accaaaatgc
     3181 atgtaccaga tgaaagcgat gtacattggc ttgttgaggc cgcagctccg aagagaaacg
     3241 gtaataaatg ttgattttct acatggcaag ccgggagatg tgcggataat aggttccggg
     3301 acgccccagg cgcccccgaa accagcagca ttctatgcca tcatttcgtt ggctatcatt
     3361 tctgcttgtt tcaaaacagt ctctgtcgcc aacatctgca tatcgggcgg ataaccatat
     3421 tttcttaaaa gccttttgac cgcaactttc aatttggctt tcacgctttc cttgatggtc
     3481 cagtcaatag aagcgttttg tcggatggtc tctgtcaatg caacggcaag ctctctcaat
     3541 tggtcttttt gcatgagatc tctcgcgctg tcattgttgg ctacggccgt ataaaaggca
     3601 tattcaaagt ctgttaaacc catggtcttc gcttcattgt ccatctcgac aatgtctttg
     3661 ctcagcttga taagctcttc gataacttcc gcagcgctga gtattttgtt gtggtatttt
     3721 ttgatggaat tttcgagcat ttccaaaaaa gtcctgctct tgaccagatt ctgttttgcc
     3781 ctcgacttga tctcatcatt cagcagtttt ttcaacactt caagcgccac attcttgtgc
     3841 tccattcctt tcagttccaa tagaaattct tctgacaaga tggaaatatc tggttttttg
     3901 atcccggcgg catcaaacac gtctatcacc tgttcagaaa ccaacgcctt atcaatgacc
     3961 tgcctgatgg tggtttcaat ttcttcatct gttctacctg agcctgtgat gtcaaatttg
     4021 gccaagcggg cttttaccgc ctgaaaaaac gaaacttcgt cttttacatc catcgcctgt
     4081 tcatgcggaa tagccagggc aaaggattta gagagggcgg tcacctcgtt gatgaagcgt
     4141 tttttgccgt cttccagacc gagactgtgt tcttcggcgg ccagaatcat agataatttc
     4201 ttagatgttt ctgcgtcaaa ataggtttca tactcaaagc catgaaacat ttgcgaaacc
     4261 acttccaatt tctccaacat caaagcaacg gcttgttcct gcaagatggt cggatcgcct
     4321 ttgccgccgg catcagcgta aaatgccaaa gcgtttttca agtctgatgc aatgcccaga
     4381 taatctacaa tcaaaccgcc cggcttatct ttagaaaccc ggtttaccct ggcaattgct
     4441 tgcatcaggt tgtgccc
//
LOCUS       DAORLF010000407         4442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10734_length_4442_cov_1.497379, whole genome shotgun sequence.
ACCESSION   DAORLF010000407 DAORLF010000000
VERSION     DAORLF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10734_length_4442_cov_1.497379"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>702)
                     /locus_tag="PK198_22645"
     CDS             complement(<1..>702)
                     /locus_tag="PK198_22645"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRF41618.1"
                     /translation="QLGCNGVIQVLLEPIRPDQPDNPLELLRRLADQEEVLAVATVFN
                     MDKQQPQPGTSAVFDRQMNLRGSISDETLLRAVQADVFQGFETQGSQVCEYHIAGKAQ
                     QVLIEICLPPPHLVLVGAGNDAWPPAQMAALLGWKVTVADGRPTHANSRRFAGGCQVI
                     VSKPESLLDSLRIHSRTAFVLMTHNYPYDLAVFRLLLQHPDIAYIGILGPRKKYLRML
                     DELGREGIRLSPEQEA"
     assembly_gap    703..729
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<730..989)
                     /locus_tag="PK198_22650"
     CDS             complement(<730..989)
                     /locus_tag="PK198_22650"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRF41619.1"
                     /translation="MREIQAILSAYEVLKKQGQPCVLATVLHVEGSSYRQAGARMLVA
                     EDGHITGAISGGCLEGDALKKALLALRQGQNKLLTYDTNDEE"
     gene            complement(993..>1745)
                     /locus_tag="PK198_22655"
     CDS             complement(993..>1745)
                     /locus_tag="PK198_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF41620.1"
                     /translation="GMGDSAVHANRFPAGAIGNYLAEEWILDTNISTGAWRAPGSNFI
                     AGAEQSFLDELAEIMGKDPIEFRLELFDKAMKNPVGKDNDYEAERYAGVLKLVREKSD
                     WANPPAGVHRGVSAYFCHNSYVAQVLDLVMKDGKPVVQKIHAAVDCGIVVNPDAAANL
                     CEGGMVDGMGHAMYSAMTFKEGTPQQSNFDKYRLIRMSEAPKAVDVHFAPSEVDPTGL
                     GEPLNPPIIGALANALYRATGKRFYDQPFFPA"
     assembly_gap    1746..1844
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1845..>2184)
                     /locus_tag="PK198_22660"
     CDS             complement(<1845..>2184)
                     /locus_tag="PK198_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRF41621.1"
                     /translation="LRKDGNPEAAFKSAAQVIERTYTAPFLAHNTMEPMNFFAHVTAD
                     KAELVGPIQTPEFMEKTVAARLGLPLEKIDIQMTRQGGGFGRRLYGHFVVEAAAISQK
                     MNAPVKLIYTR"
     assembly_gap    2185..2276
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2277..>2599)
                     /locus_tag="PK198_22665"
     CDS             complement(<2277..>2599)
                     /locus_tag="PK198_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRF41622.1"
                     /translation="LFGLDYHREGMLIAMLVHPPAFGLKLKSVDDSAAKAMPGIKDVF
                     TMKVYNDGYEKQWSDTEAFNEKVVVVGNTTWEVMQAKKALKLEWEAVAGYAENVQGWG
                     GPQKV"
     assembly_gap    2600..2718
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2719..>2958)
                     /locus_tag="PK198_22670"
     CDS             complement(<2719..>2958)
                     /locus_tag="PK198_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRF41623.1"
                     /translation="EQAPLNTDVFTRQLAGGSQSIRQGWESLRMAGATARKMLREAAA
                     KAWNVPIDEISTEAGMLHHKNSGKSAGYGEMASAAA"
     assembly_gap    2959..2973
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2974..3255)
                     /locus_tag="PK198_22675"
     CDS             complement(<2974..3255)
                     /locus_tag="PK198_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRF41624.1"
                     /translation="MTTVKTTYNRRSFIKSTVLAGGGMMLGFSWLVSCKPSPEQLLEM
                     PKEWFNINGFLKIGENGVVTIMSPNPEIGQNVKTSMPMIVAEELDVDWKQ"
     gene            complement(3313..3768)
                     /locus_tag="PK198_22680"
     CDS             complement(3313..3768)
                     /locus_tag="PK198_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRF41625.1"
                     /translation="MASFSLNINGQSRQVDVDPSTPLLWVLRDHLQLVGTKYGCGIAQ
                     CGACTVHLGDVAMRSCILPVSQVGNQKVTTIEGLSENGDHPLQEAWLEHDVPQCGYCQ
                     AGQIMTAAALLKSNPRPSDEEIEATMHGNICRCGTYTRIKKAIQTAAGK"
     assembly_gap    3907..4146
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4147..>4442)
                     /locus_tag="PK198_22685"
     CDS             complement(<4147..>4442)
                     /locus_tag="PK198_22685"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRF41626.1"
                     /translation="GMRIATISIGYADGLLRAAGNGRFSVLIRGRRAPTVGNICMDMC
                     MADISQIPDAGEGDPVVVFGPGLPLEELAAALGTIPYEVLTGISPRVKRVYVQE"
BASE COUNT          707 a         1081 c         1148 g          914 t
ORIGIN      
        1 ggcttcctgt tccggcgaaa gccgaatgcc ctcgcggcca agctcgtcca acatgcggag
       61 gtattttttt cgcggcccca gaatgccgat gtatgcgatg tcggggtgtt gcaggagcaa
      121 ccggaacact gccaggtcgt aggggtaatt gtgcgtcatc agcacgaagg cggtgcgcga
      181 atggatgcgc aggctgtcga gcaggctttc cggtttcgat acgatgacct ggcacccgcc
      241 ggcaaaacgg cggctgttgg cgtgcgtggg gcggccatcg gctaccgtaa ctttccatcc
      301 gaggagggcg gccatttgtg cgggcggcca tgcgtcgttg cccgcgccta cgagtacgag
      361 atgcggcggc ggcagacaaa tttctatcaa aacctgttgc gccttgccgg caatatggta
      421 ctcacagact tgggagcctt gtgtttcaaa accttgaaaa acatcggctt gtacggcccg
      481 gagcagggtc tcgtctgaga tgctgccgcg caggttcatc tgccggtcga acacggcgga
      541 ggtgccgggc tgcggctgct gtttgtccat attgaaaacc gtggctacgg ccaatacctc
      601 ctcctgatcg gcgagccgtc gcaggagttc cagcgggttg tcgggctgat cggggcggat
      661 gggttccagc aagacctgta tgacgccgtt gcagcccaat tgnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnt cctcttcgtc gttggtgtcg taagtgagga gcttgttttg cccctgccgc
      781 agggccagca atgctttttt gagggcgtcg ccttcgagac agccgccact gatggccccg
      841 gtgatgtggc cgtcttcggc aacgagcatg cgcgcgcctg cctgtcggta cgaagagcct
      901 tcgacgtgca gcacggtggc caatacgcag ggctgcccct gttttttgag gacctcgtaa
      961 gccgagagga tggcctggat ttcgcgcatg gactatgccg ggaaaaacgg ctgatcataa
     1021 aagcgcttgc cggtggcccg gtacagggca ttggccagcg cgccgatgat gggcggattg
     1081 agcggctcgc ccagaccggt gggatccacc tcgcttgggg caaagtgaac gtcaaccgct
     1141 ttgggcgctt cgctcatgcg aatgagccgg tacttgtcga aattgctctg ctgcggcgtg
     1201 ccttctttga aagtcatggc gctgtacatc gcgtggccca tgccgtccac cataccgcct
     1261 tcgcaaagat tggcggccgc atcgggattg accacaatgc cgcaatcaac ggcggcgtgt
     1321 attttctgca ccaccggttt gccatccttc atcaccaggt ccagcacttg cgccacatag
     1381 ctgttgtggc agaaataggc cgacacgccc cggtgtacgc cggcgggcgg attggcccaa
     1441 tcggattttt ctctgaccaa cttcaaaacg cctgcatagc gctcggcctc gtaatcgttg
     1501 tctttaccga cagggttttt catggccttg tcaaataatt ccaaacggaa ttctatgggg
     1561 tctttgccca tgatttctgc cagttcatcg aggaaggatt gctctgcccc cgcgatgaag
     1621 ttcgaccccg gcgcgcgcca tgcccctgtg ctgatgttgg tatccaatat ccattcttcg
     1681 gccaagtagt tgccaatagc tccggcggga aagcggttgg catgcacggc gctgtcgccc
     1741 ataccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgggt atagatgagt
     1861 ttgacgggcg cgttcatttt ttgggaaatg gctgctgctt ccaccacgaa atgcccatag
     1921 agcctgcgcc cgaagccgcc accctgtctt gtcatctgaa tatcaatctt ttcgagcgga
     1981 aggccgagcc gggccgctac cgttttttcc ataaattccg gtgtctggat cggccccacg
     2041 agttcggctt tgtcggcagt gacgtgggcg aagaagttca tcggctccat cgtgttgtgg
     2101 gccaggaagg gcgcggtata ggtgcgctcg atgacttgtg ccgccgattt gaacgcagcc
     2161 tccggattgc cgtctttgcg cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcac
     2281 tttttgcggg ccgccccagc cttgcacgtt ttcggcatag ccggcaacag cctcccactc
     2341 caatttcagc gcttttttgg cctgcatcac ttcccaggtg gtattgccca ccaccactac
     2401 tttttcattg aaggcctcgg tatcgctcca ttgtttttcg tagccgtcgt tataaacttt
     2461 catggtaaag acgtccttga tgcccggcat ggccttggcg gcggagtcgt ccaccgattt
     2521 gagtttcaaa ccgaatgccg gcggatgcac gagcatggcg atgagcatgc cttcgcggtg
     2581 gtagtcgagg ccaaagagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnngg cggcggcaga agccatctcg ccataaccgg ctgattttcc
     2761 gctgtttttg tggtgcaaca tgcccgcctc tgtactgatt tcatctatgg gaacattcca
     2821 ggctttggcg gctgcttcgc ggagcatttt gcgggcggtg gcgccggcca tgcgcagcga
     2881 ttcccaaccc tgccggatgg actgactgcc gccggccaat tgccgggtaa acacgtcggt
     2941 attgagcggc gcctgctcnn nnnnnnnnnn nnnctgcttc cagtccacgt ccaattcctc
     3001 ggccacgatc atgggcatgg aggttttgac gttttgcccg atttccgggt tgggcgacat
     3061 gatggtgacg acgccgtttt cgccgatttt caaaaatccg ttgatattga accactcctt
     3121 tggcatttcc aaaagttgtt cggggctggg cttgcaggat accaaccaac tgaatcccag
     3181 catcatgccg cctccggcca gcacggtgga tttgataaaa gagcgcctgt tgtatgttgt
     3241 tttgacggtg gtcatggtgg ataggcagtt ttgcggtttt acatttaata ttttacattt
     3301 tgcggttaga ccctactttc cggccgccgt ttgaatggct tttttgatgc gggtgtaggt
     3361 accgcagcga caaatattgc cgtgcatggt agcctcgatt tcctcgtcgc tggggcgagg
     3421 attggatttc agcagggcgg cggcggtcat gatctggccg gcctggcagt agccgcactg
     3481 gggcacgtcg tgttccagcc aagcctcttg cagcgggtgg tcgccgtttt ccgacaggcc
     3541 ctcgatggtg gtgacttttt ggttgcccac ctgcgagacg ggcaggatgc aggagcgcat
     3601 ggccacgtcg ccgaggtgga cggtgcaggc tccgcattgg gcgatgccgc agccgtattt
     3661 ggtacccacg agttgcaggt ggtcgcgcag cacccagagc agcggcgtgg aagggtccac
     3721 gtccacttgt cgggattggc cgttgatgtt gagagagaag cttgccatag tggtgtgtgt
     3781 tgatgaaggt aacaggatgt aaatatacgt tagatcgtaa ctactcagta tttttttcaa
     3841 agccacaagc tgctggctat tagctgttag cttgtttttt cgttactatt cagcgggcct
     3901 ccaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnncact cctgcacata cacccgcttc acgcgcggcg aaatgcccgt gagcacctcg
     4201 tagggaatgg tgccgagggc ggcggcgagt tcttccaacg gcagacccgg tccaaacacc
     4261 accacaggat caccctctcc ggcatcgggt atttggctga tgtcggccat gcacatatcc
     4321 atacaaatgt tgcccaccgt aggcgcccgc ctgccccgga tgagcaccga aaaacgcccg
     4381 ttgccggcag cgcgcaacaa gccgtctgcg tagcctatgc tgatggtggc aatgcgcata
     4441 cc
//
LOCUS       DAORLF010000408         4440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10743_length_4440_cov_1.760091, whole genome shotgun sequence.
ACCESSION   DAORLF010000408 DAORLF010000000
VERSION     DAORLF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10743_length_4440_cov_1.760091"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    341..381
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <382..>2260
                     /locus_tag="PK198_22690"
     CDS             <382..>2260
                     /locus_tag="PK198_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41627.1"
                     /translation="YANDEALNVFELDRLNKPWQLKYDSLRIEMANAGDTPGEALRNE
                     FEYYKKYQKYYIRWRARVYPYMQQDGSIDFSIKLEKETPIPYLKSNTANWSFLGPVVT
                     RWRQNDNAAQPLAPFQANVYCLDVAPSNGNIVYCGTETGVVNKSIDKGLTWTTVGANV
                     FTNNIGAIAIHPTNPDIVYVSEFSRYIRSTTDGGASWQTVLDISSFNCEDIKIKPDEP
                     EVVLASGNSGLRRRTAGNVWTSVISSKTYDIAFQPGNPLVAYALVRNAGADLCEFWKS
                     TDGGQTWSVRSTGWISGAGFTDGGGRITVTPAAADRIYCALLTGNGPRIMRSDDAGET
                     WTVIAASNQTGLIGPCTNGPLSMTNGQGFFDLSIVASHDNADHIIVGTTTSFKSTDGG
                     STWATLGGYCGPFQIHPDIQEAIAHGTDTWIVTDGGLNLSTDFFTNPAVGVNYFVRTN
                     NLRGSEHWGFDIGWNEDVVVGGRYHNGNTAWREGYPAGDYLRMGGGEAPTGYVNPGNN
                     SLTYFSDLGGRILPANNTQAVGSFTVSKWPNESFYPMEGAEQKWDPRYMYTYYLGQGH
                     QLWKTTDNGLSFTPIFTHLNSDAKVRYIEVSRSNPDFIYITVYLPSPSTDGQLWKTDN
                     GG"
     assembly_gap    2261..2271
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2333..>3766
                     /locus_tag="PK198_22695"
     CDS             2333..>3766
                     /locus_tag="PK198_22695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41628.1"
                     /translation="MSGTDHNTIWWAFRTGPNGQKIFKSTDGGATWTNWTTSTLNNID
                     ATDILHQLGTDGGVYFVSGNGAKVYYRDNSANDWMAYDTGLPLNMYGDFGGAFLKAFY
                     KGGKLRLAGGHGIWEADLCAVSTTTLVQPMVDNPAPTCSRDTIQLESYSVINGNASYQ
                     WSLSPAPQWISDANARNPRVVLGSTPGAYSVTLSVTDDNGTATRTINDMIQNLATGNL
                     CGADHVPGKALSLDGSGDFAQANTPLNLNSNTVTMTAWVKRNGAQVDFSGLVYARGGT
                     TSAGLSITSSNQLRYTWDDASGSYNFNTGFTIPDNVWTHIALVITSASAKVYMNGIAA
                     TRNATHNAEAFDTPLKIGYDQGSRYFKGEIDEVTVWNVSLTQEQIRELMHLTIEPADW
                     PGLVSYYQFNEPSGNANDKIGTRHAALAADATRMVSTGPFGGGASHRKTITGGGVNAF
                     TGTDVTLSFPATGTYPNGEIVVSRINNA"
     assembly_gap    3767..3777
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3976..>4440
                     /locus_tag="PK198_22700"
     CDS             3976..>4440
                     /locus_tag="PK198_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41629.1"
                     /translation="MPGSSTEAQGGANGSITFGTGSGITQFGQFVASNTLVGQFITGP
                     ASVCSGSNATLDAGAGYSSYNWSNGGGSGQMATFNNLSTATTIFATVTDNDGCSSESS
                     FTVGITPVQIWYADADNDGFGDPAVSQMSCTQPSGYVADNTDLCPTDINKQAP"
BASE COUNT         1115 a         1203 c         1078 g          981 t
ORIGIN      
        1 gacgagttgt taaacagtaa atgatttgat ttttttgtaa aaaaacggct ggggcagcaa
       61 tgcttcagcc gtttttgttg gtgcgttttt aataaaaaaa ttgcaccgcc gttcgctacg
      121 gccatttgca atatacacat tatcaattac ataaaaggtt gtcccccttt ttgtccccct
      181 tcctgatgca gggccggttc tttgtaattc ctacaatgag tatatttgca ttttgcttag
      241 tatcagtaac aaatcagtat ctcatgaaaa aatgctacct tgctttcatg ctcttgtgca
      301 tggcgtactt tggctattcc caaccactca acacactcgg nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ngtacgcgaa tgacgaggcc ctgaacgtct ttgaattaga
      421 ccggctcaac aagccctggc aactgaaata cgactctctg agaatagaga tggccaatgc
      481 cggagatacg ccgggagagg cgctgagaaa cgagtttgaa tattacaaaa aataccagaa
      541 atactacatc cgttggaggg cgagggtgta tccttatatg cagcaagacg gttctattga
      601 cttctccata aaattggaaa aagaaacccc gataccatat ctcaagtcga atacggccaa
      661 ttggagtttc ctcggcccgg tagtcacccg atggagacaa aacgacaatg ccgcacagcc
      721 gcttgccccc tttcaggcca acgtatattg tctggatgtg gcgcccagta acggcaacat
      781 tgtctattgc ggcaccgaaa cgggtgtcgt caataaaagt attgataaag gtcttacctg
      841 gacgaccgtg ggagccaatg tttttaccaa caatataggg gctattgcca ttcatcctac
      901 caacccggac attgtgtatg tgtctgaatt ttcgagatat atacgatcaa ctaccgacgg
      961 cggcgcaagc tggcaaaccg tacttgatat ttcgagcttc aactgcgaag acatcaaaat
     1021 aaaacccgac gaacccgaag tggtattggc ttccggcaac agcggcctgc gacgccgcac
     1081 agcaggcaat gtctggacca gcgtcatcag cagcaaaacg tacgatattg cctttcagcc
     1141 cggcaacccc ttggtggcgt atgccttggt cagaaatgcc ggcgctgatc tttgcgaatt
     1201 ctggaagtca accgacggcg gccaaacctg gtcggtgcgc tctactggtt ggatttcggg
     1261 agctggcttc acagacggtg gaggccgcat caccgttacg cctgccgctg ccgaccgcat
     1321 ctactgcgcc ctgcttaccg gcaatgggcc acgcatcatg cgcagcgacg acgccggcga
     1381 aacatggacc gtcatagctg ccagcaacca aaccgggctt atcggccctt gtaccaacgg
     1441 ccccttgagt atgaccaacg gccagggctt tttcgatttg tctattgtgg catcgcacga
     1501 taatgcagac catattattg taggcaccac gacttccttc aaatcaaccg acggtggttc
     1561 cacttgggct acgcttggcg ggtattgcgg cccgtttcaa atacaccccg acatacagga
     1621 agcaatagcc cacggcaccg acacctggat tgtaaccgac ggcggcctca acctctctac
     1681 cgacttcttt accaaccctg cggtgggcgt caattatttt gttcgtacca ataacctgcg
     1741 cggctcggaa cactggggct tcgacatcgg ctggaatgaa gatgtggtag tgggcggcag
     1801 ataccacaac ggcaataccg cctggagaga agggtatccc gctggagatt acttgcgcat
     1861 gggcggtggt gaagccccca ccggatatgt caatcccggc aacaattcgc tcacttattt
     1921 ctccgatctg gggggaagaa ttttacccgc caacaacacg caggcagtcg gctcttttac
     1981 tgtcagcaag tggcccaacg agagctttta ccctatggag ggcgccgagc aaaaatggga
     2041 cccgcgctat atgtacacct attatctggg acagggccat cagctttgga aaacaaccga
     2101 caacgggctg tcatttacgc cgattttcac acacctcaac agtgatgcca aagttcggta
     2161 tatagaagta tcgcgcagca atcctgactt tatttacata acggtttatt taccaagccc
     2221 ttccaccgac ggtcagcttt ggaaaaccga taatggaggc nnnnnnnnnn ngatacagtg
     2281 tgccaacccc ggcacgctga cggcaagcca gcgccgtttt tcaaaaataa ccatgagcgg
     2341 cacagaccac aatacaattt ggtgggcgtt tcggaccggc cccaacggcc agaaaatatt
     2401 caagtccacc gatggcggcg ctacctggac caattggacc acctctaccc tcaataatat
     2461 tgatgcaacc gatattcttc accagttggg tacagacggg ggcgtttatt tcgtcagcgg
     2521 caatggcgcc aaggtatatt accgcgacaa cagcgccaac gactggatgg cctacgacac
     2581 cggcctgccg ctcaatatgt acggcgattt cggaggcgct tttctgaaag ccttctacaa
     2641 aggcggcaag ctcaggctcg ccggcgggca tggcatttgg gaagctgatc tctgtgccgt
     2701 ttctaccacc acattagtac aacctatggt ggacaacccc gcgcccacct gtagcagaga
     2761 caccattcaa ttggaaagct actcggtcat caacggcaac gcaagctacc aatggtcgct
     2821 ttctccggcg ccgcaatgga tcagcgacgc caatgcccgc aatccccgcg tggtgttggg
     2881 cagtactccg ggcgcttatt ccgtcacact ctccgtcacc gacgacaacg gtactgcgac
     2941 tcgtacgatc aatgacatga tccagaatct ggcaacgggc aacttgtgcg gcgccgacca
     3001 tgttcccggc aaggccctgt cgttggacgg cagcggagat tttgcgcagg ctaacactcc
     3061 gctcaacctc aattccaata ccgtgactat gaccgcctgg gtgaaacgca acggcgctca
     3121 ggtggacttc tcaggcttag tgtatgcccg cggcggcacc acaagcgccg gcttgagcat
     3181 tacatcgagc aaccaactgc gctacacttg ggacgatgct tcaggcagct ataattttaa
     3241 tacaggtttt accataccag acaatgtctg gacgcatatt gcattggtca ttacctccgc
     3301 cagcgctaag gtgtatatga atggcatagc ggccactcgt aatgctacac acaatgcgga
     3361 agcattcgat acgcccttga aaatcggcta cgatcagggc agccgttatt tcaaagggga
     3421 aattgacgaa gtaaccgtgt ggaatgtgag cctcacacaa gagcaaatac gcgagctgat
     3481 gcacctcacc atagagcctg ccgattggcc cggtttggtg agttattatc agttcaatga
     3541 gccttcgggc aacgccaacg ataaaatagg aacccgtcac gctgctttgg ctgccgatgc
     3601 aaccagaatg gtctcaacag gacctttcgg aggaggagca tcgcatcgga aaaccattac
     3661 aggaggaggc gtcaatgctt ttacaggaac agatgtaacc ctgagtttcc cggctacggg
     3721 cacttatccc aacggtgaaa ttgtggtgag cagaatcaac aatgccnnnn nnnnnnntcc
     3781 atccccgctt gcagcgcgta tctgtctgat aatcattatt ggatcattga caattacggc
     3841 tccaaccaaa ctttttcggc gctcgaatcg gccatattcg gcaacatttc cgtgccttca
     3901 acggcaagca tcccggctat ttcgtataaa ctgaaccaca gaacagtcaa tgctcatttg
     3961 ttgccctggg cactgatgcc gggtagctcc accgaggcgc aaggcggggc caacggcagc
     4021 attacgtttg gaaccggttc gggcattacc caattcggcc agttcgtcgc aagcaatact
     4081 ttggttggtc aattcatcac agggccggcc tcggtttgca gcggatcaaa cgccacattg
     4141 gatgcaggcg ccgggtacag cagctataac tggtcgaatg gcggaggaag cggccaaatg
     4201 gctacattca acaacctcag caccgcaacg accatatttg caacagtgac cgacaatgat
     4261 ggatgcagtt ccgagtcttc tttcacagta ggaattacac cggttcaaat ctggtatgcc
     4321 gacgcagaca acgacggctt cggagatccc gccgttagcc agatgtcttg cacccaaccc
     4381 tcaggatatg tagccgataa cacggacctg tgtcctacag acatcaacaa acaagctccc
//
LOCUS       DAORLF010000409         4422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10790_length_4422_cov_1.811999, whole genome shotgun sequence.
ACCESSION   DAORLF010000409 DAORLF010000000
VERSION     DAORLF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10790_length_4422_cov_1.811999"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>349
                     /locus_tag="PK198_22705"
     CDS             <1..>349
                     /locus_tag="PK198_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol 3-phosphate dehydrogenase"
                     /protein_id="HRF41630.1"
                     /translation="AMLITRGLTEMIYLGKAMGATPRAFLGTAGIGDLVATATSKNSR
                     NFTFGFRLGSGESRAEIEATMPELAEGVRTLYIAHQLAAYYKLRIPITKMLYEIVFND
                     FDISKAIDMLIGYP"
     assembly_gap    350..407
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            453..>717
                     /locus_tag="PK198_22710"
     CDS             453..>717
                     /locus_tag="PK198_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF41631.1"
                     /translation="MSEKILILDFGSQYTQLIARRVRELNIFCEIFPFNHYPALDESV
                     KGVILSGSPFSVTDENALHIDLQQIIGKVPVLGVCYGAQYIAQY"
     assembly_gap    718..804
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <805..>1039
                     /locus_tag="PK198_22715"
     CDS             <805..>1039
                     /locus_tag="PK198_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF41632.1"
                     /translation="IPEGDQVWMSHGDTIIQIPEQFELLAGTNSIEVAAYKSKEAAFE
                     APVYCIQFHPEVTHSLSGKQLLQNFLYDIADCKG"
     assembly_gap    1040..1158
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1159..>1968
                     /gene="guaA"
                     /locus_tag="PK198_22720"
     CDS             <1159..>1968
                     /gene="guaA"
                     /locus_tag="PK198_22720"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRF41633.1"
                     /translation="IGEGLICFFIDNGLLRKNEFEEVLDSYRTMGLRVVGIDARQRFW
                     DALSGLTNPEDKRKAIGRLFIEVFEEEAHKIEQVQWLGQGTIYPDVIESVSVHGPSVT
                     IKSHHNVGGLPDKLKLKIVEPLRMLFKDEVRRVGEELGIPKNILHRHPFPGPGLGIRI
                     LGEVTPERVALLQEADAIFINHLKKSGLYEQVWQAGVVLLPIYSVGVMGDERTYEQAV
                     ALRAVNSSDGMTAEWSRLPHDFLAAVSSEIINHVKGINRVVYDISTKPPSTI"
     assembly_gap    1969..1978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1979..>3178
                     /locus_tag="PK198_22725"
     CDS             <1979..>3178
                     /locus_tag="PK198_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41634.1"
                     /translation="GNNGLVTIVFLLLVFQLTACELFTKVQPGKDATGGKDVLDPIQG
                     TRVYDPETGTYIVVKTAPTQPMDTVKWKEISMTASPPITSEGVFVDREPSNPVVTTVD
                     PATGSSKLETYNVAIVLPFQTDKFTAQGQVPANSSWALNFYSGAKMALDELRAEGGPN
                     LNVAVLDTKATESGMMELTRRNEFTSAHLIIGPYRRETVRIAADAAKRNNSVLVSPYN
                     ATENIVDNNPNYIQVNPSFSTHCDALLSEARHRFRPDQIVLVCRGRDVELERLKYFQD
                     AHKRMQLQSFSRDTAALREFVIADAADSSAFRINVQPLVQNRDTLAIIIPSWSSESFI
                     NALLQQLHVAKSGYSHFTVYGMPQWIEYERVSHQYYESLNLHITTSSHVDREQWAVQD
                     FQKSYFTR"
     assembly_gap    3179..3228
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3229..3445
                     /locus_tag="PK198_22730"
     CDS             <3229..3445
                     /locus_tag="PK198_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41635.1"
                     /translation="TAYYVRMLRKYGTRFQYAIIDDAGQGLHTRFEVRPIAMPNASNR
                     EFPQVKQFENKYVNLLRFEDLRFVKVN"
     gene            3679..4275
                     /locus_tag="PK198_22735"
     CDS             3679..4275
                     /locus_tag="PK198_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF41636.1"
                     /translation="MKKSILFSLAAAVVAAFALAFTPPVESMKVDTAASVVAWKGAKV
                     TGTHNGKISVKSGKLDFNNGVLTGGSFEIDMNSITCEDMSGGGAAKLVGHLKSDDFFG
                     AEKYPTSKFVITQVVSRGTTGAYKVVGDLTIKSTTKSIRFMADVKEEGGKMVATAKIT
                     IDRSEFDVRFGSGSFIDNLGDKTIYDEFDLDIKLVASK"
BASE COUNT         1029 a         1047 c         1028 g          994 t
ORIGIN      
        1 ggccatgctc atcacgcgcg gtctcaccga aatgatctat ctgggcaagg ccatgggcgc
       61 tacgccgcgc gcttttctcg gcacagccgg catcggcgat ttggtggcaa ctgcaacatc
      121 caaaaatagc cggaatttta ccttcggctt tcgtttgggg tccggcgaaa gccgtgcaga
      181 aattgaggct accatgcccg aattggcaga gggcgtgcgt acattgtaca tcgcgcatca
      241 gttggcggcg tactacaaac tccgcattcc cattaccaaa atgttgtacg aaatcgtatt
      301 caatgatttc gatatttcaa aagccataga catgctcatc ggctaccccn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt ccctttttgt
      421 gcatcttcca aagttctgat tatctttgcc gcatgagcga gaagatactg attctggatt
      481 tcggttctca atatacccaa ttgattgcca ggcgggtgcg ggaactcaat attttttgtg
      541 agatatttcc cttcaaccac tacccggcat tggacgaatc ggtcaaaggg gtcatcctct
      601 ccggcagccc gttctcggta acggacgaaa atgccttgca tattgacctt caacaaatta
      661 tcggcaaggt gccggtgttg ggcgtatgct acggcgcgca gtacattgcc caatattnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnncatccc cgaaggcgac caggtgtgga tgtcgcacgg
      841 cgatacgatc attcaaatac cggagcaatt tgagttgctg gccggcacca acagcataga
      901 ggtggcggca tacaaatcga aagaagcagc ctttgaagcg ccggtgtatt gcattcagtt
      961 tcacccggaa gtaacgcaca gcctgagcgg caaacagttg ctgcaaaatt ttttatacga
     1021 tattgcagat tgcaaaggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnat cggtgagggg ctgatttgct tttttataga caacggcctg
     1201 ttgcgcaaaa acgaattcga ggaagttttg gattcgtatc gcacgatggg gctgcgcgtg
     1261 gtaggcatag acgcccgcca aaggttctgg gatgcgctca gcggtctgac caacccggaa
     1321 gacaaacgca aggccatcgg gcggcttttt attgaagttt ttgaagagga agcgcacaaa
     1381 atagagcagg tgcaatggct ggggcagggt accatctacc ccgatgtgat cgaatcggtg
     1441 tcggtacacg gcccctcagt aaccatcaag tcgcaccaca atgtaggcgg cctccccgat
     1501 aagctcaagc tgaaaatcgt ggagccgttg cgcatgttgt tcaaagacga ggtgcgtagg
     1561 gtaggagagg agttgggcat tcccaaaaac atcctgcacc gccatccttt tcccggcccc
     1621 ggcttgggca tccgcatcct cggcgaagtg acgccggagc gggtggctct gttgcaggaa
     1681 gccgatgcca tttttatcaa ccacctcaaa aagagtgggt tgtatgagca ggtatggcag
     1741 gccggcgtag tattattgcc catttattca gtcggcgtta tgggcgacga gcgcacctat
     1801 gaacaagcag tagccttgcg cgccgtcaat tcatcggacg gtatgactgc cgagtggagc
     1861 cgcctgccgc acgatttttt agctgctgtt tccagcgaga tcatcaacca tgtaaaagga
     1921 ataaaccgag tagtgtatga tatcagcacc aaaccgcctt ccactattnn nnnnnnnngg
     1981 gaataacggc ctcgttacca tcgttttttt gctgttggtt ttccaactga cggcctgcga
     2041 actgtttacc aaagtacagc cgggcaaaga cgctaccggc ggcaaagacg tattggaccc
     2101 catacagggt acgcgggtat atgaccccga aaccggtaca tacatcgtgg tgaaaaccgc
     2161 gcccacccag ccgatggaca cagtgaagtg gaaggagatt tccatgactg cctcgccacc
     2221 catcacttcc gaaggtgtct tcgttgaccg cgagccttcc aatcccgtag tgacaaccgt
     2281 ggacccggca accggctctt ccaagttgga gacctacaat gtagccatcg ttttgccctt
     2341 ccaaaccgac aaattcacgg cgcaggggca ggttccggcc aattcttcct gggctttgaa
     2401 tttttactcc ggcgccaaaa tggccttaga cgaacttcgc gccgaaggag gccccaatct
     2461 gaacgtagcc gtgcttgata ccaaagctac cgaatcgggc atgatggaac tgacccgccg
     2521 caacgagttc acgagtgcgc acctcatcat cgggccatat cgccgcgaga ccgttcgcat
     2581 tgccgccgac gccgccaaac gcaacaattc ggtgctcgtt tctccttaca acgccacaga
     2641 aaatatagtg gacaacaacc cgaactatat tcaggtaaat ccttcattct ccacgcattg
     2701 cgatgctttg ctcagtgaag cccgccatcg tttccgcccc gatcaaatcg tattggtgtg
     2761 ccgtggccgc gatgtggaac tggaacgcct caagtatttt caggacgcgc ataagcgcat
     2821 gcaattacag tctttttcga gagatactgc ggcactccgg gagtttgtca ttgccgatgc
     2881 ggccgacagc agtgctttcc gtatcaatgt gcagcctttg gtgcaaaacc gcgacacatt
     2941 agccattatc attccttcct ggtcgagtga gagttttatc aacgcattgc ttcagcaatt
     3001 gcacgtcgcc aaaagtggat actcgcattt cacggtttat ggtatgccac aatggattga
     3061 atatgagcga gtcagtcatc aatactatga gtcgctcaac ctgcacatta ccacgagttc
     3121 gcatgtggac cgcgagcaat gggcggtgca ggacttccaa aaatcatact tcacccgcnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cggcctacta
     3241 tgtgcggatg ttgcgtaagt acggcacgcg tttccagtat gccatcattg acgacgccgg
     3301 acaagggctg cacacacgct tcgaggtgcg tccgatagcc atgccgaatg cctccaatcg
     3361 cgagtttcca caggtgaagc agttcgagaa caagtacgtc aatctcttgc gcttcgagga
     3421 tctgagattc gtgaaagtga attgaaggat atttcagact ttgtaaggtt ttcaaaatct
     3481 tacaaagtct gaaattttct catcgaaagc tttctgacaa attatacccc atacttccac
     3541 attgcaggcc gatctcctgt ttttcccaat tccttaattt tttgttaaaa atacatgtac
     3601 ctacatctat tttttaaact atcctgatga agaggcgttc ttgcggcatt atttcaccca
     3661 actaaaaaat gagcaaacat gaaaaaatca attctcttct ctttggcggc agcagtagta
     3721 gctgcattcg cattggcttt tacgcctccg gtagaaagca tgaaagtgga cacggcagcc
     3781 agcgtggtag cctggaaagg cgctaaggtg accggcactc acaacggtaa aatttctgtg
     3841 aaaagcggta agctcgactt caacaacggc gttctgacgg gcggcagttt cgagattgac
     3901 atgaactcca tcacttgtga ggatatgtcg ggcggcggcg cagcgaaatt ggtgggccac
     3961 ctcaagtcgg acgacttctt tggcgcagaa aagtacccca cctctaagtt tgtcatcacg
     4021 caagtggttt cccgcggcac gacgggtgct tacaaagttg tgggcgacct cacgatcaag
     4081 tccactacca agtccattcg ctttatggcc gatgtgaaag aagagggcgg caagatggtt
     4141 gctacggcta aaattacgat tgaccgctct gagtttgacg tgcgcttcgg ttcgggcagc
     4201 ttcattgaca accttggcga caagacgatc tacgacgaat tcgatttgga catcaaactt
     4261 gttgcgtcca agtaatcagg atatagtatt aaagttgaac aacacaacag agcatcccgg
     4321 attcaaaatt cgggatgctt ttttttgccc attaagattt gataaacctt agtagtgggg
     4381 caaaaataaa gtgttcattt tctttaggcc aaaggattag at
//
LOCUS       DAORLF010000410         4393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10889_length_4393_cov_2.057400, whole genome shotgun sequence.
ACCESSION   DAORLF010000410 DAORLF010000000
VERSION     DAORLF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10889_length_4393_cov_2.057400"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>808
                     /locus_tag="PK198_22740"
     CDS             <1..>808
                     /locus_tag="PK198_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41637.1"
                     /translation="FTIPHSTPFFLNATASDPNGHPLTYCWEQMDLGPAGVPVATNTT
                     GPMFRSRPPSASPTRYFPRLEDVVSGAATPWEVLPSVARTLNFRVTVRDWTGSYGCSA
                     VANTTVTVSSSGPFAVTQPNTNVSWQGGQTYTVNWNVVGSDAYCPNVDILLSTDGGLT
                     YPTTLATATPNDGSHDVTIPTIPSMLITARIMVRGAGHIFYDISNVNFTLLAGVLPVE
                     LLYFEAAAEGKTAQLRWATATERNNAGFRIERSEGQSGGFQSIGWVAGKGD"
     assembly_gap    809..818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <819..1193
                     /locus_tag="PK198_22745"
     CDS             <819..1193
                     /locus_tag="PK198_22745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41638.1"
                     /translation="TENRYGFTDTEVKSGQTYYYRLRQTDFDGAETLSDIQAVRMAGA
                     ADDILVYPNPALDELYVQWSQPDEKGVQLEVLDVQGRPLGLWAITESVHRVDTRQWAS
                     GQYILRVVTGGKVWLKRVLIRR"
     gene            1443..1664
                     /locus_tag="PK198_22750"
     CDS             1443..1664
                     /locus_tag="PK198_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41639.1"
                     /translation="MKKFYQPAQVGSFHRSLSLGFSDARKFLPGICSLFFKSGQAAPF
                     LSVSVQYFTRLDGFAFAKSVPSGGHFLLK"
     gene            1701..>4393
                     /locus_tag="PK198_22755"
     CDS             1701..>4393
                     /locus_tag="PK198_22755"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF41640.1"
                     /translation="MATFYHTDFPHEKKTSDRSVQEPARGIETCSKTTINLFLMNQLS
                     TLLPKWSARFSAPVTLVLGMLFAATTAMGQISVTATTACIDNPGGTPAALNQYYVRVT
                     GLTGGTSPYFVSIDGVSQTYNTGDPALVFGPYTHSGAGNAVQVISVVDSDPLLPLFTT
                     AEVVEVLCGYTTNNGLNASGVSCVPATAPSANGYYGMILAQSAPGSFASGGTSGQTQA
                     YILVLNDFIVQTNFTGLFTNLAPGDYQVYAINYRTSEAATINGFLLPGEAVQPIVDGA
                     NGTGPLVGVCYTLCGPAFYTVGPCYSVGSHVFADLDDNAIFNPGTENGLPGIPVQLFA
                     QGADPTTATPVASTITNASGIYFFGGLPAVSYFIYIPTPNAAYPSSSTNGPNINSQTT
                     DNDDNGLQTTSGGAVTSIVFTLGPGMPTTEPGPGGTQDDLDDARGNMTLDFGFAPAPM
                     ITHFKMLSGITQTGHNAYTVTYTVEVRNNGAAGTYHLYDTPNFENNITITGGGYTSNF
                     AGGPSGSFTAPYNGTQNQLANNVSIALGAIHIYTVSFNVTIDMLGANTFPTFDTYTRC
                     GGGVGGPPVAGQGLFNLSQLDRGADGSIEETRTACGDLPYITHEKLGPAISTLQPDGS
                     YNVTYTIRVYNAGGVAGNYDLSDTPYFDDDITINTASYSTMSVVPGVVGGALSLNSGV
                     LNALANDQPIAAGANHTYTLIYNVTLDLSPTSTDGGNNTYTSCGTVSTAPGPLTPGST
                     VTTRMGLYNTSALDTNNDANPDEVDERCGDLPFLSLAKTFVSAVPTAAAGAYTVTYTI
                     TVMNTGGATTTYDLRDVPGFDDDIAITSASYAGVGVGAPAGAALAGSGPWLLADDASI
                     AAGVTHTYTLMVNVLLDLSAGSSGNNSYTACGTTQGSTSPIP"
BASE COUNT          978 a         1179 c         1123 g         1103 t
ORIGIN      
        1 cttcaccatt ccccacagca cgcccttctt cctcaatgcc acagcctccg accccaacgg
       61 gcacccgctc acctactgct gggagcaaat ggacttggga cctgccgggg taccggttgc
      121 taccaacact accgggccga tgtttcgcag ccgtccgccc tcggcatcgc ccacgcgcta
      181 ttttccacgt ttggaagatg ttgtttccgg ggcagccacg ccctgggagg tattgccctc
      241 ggtggcccgc accctcaatt ttcgggtcac ggtacgcgac tggacgggca gctatggttg
      301 cagcgcggtg gccaatacaa cagttaccgt gagcagcagc ggccccttcg ccgtcacgca
      361 gcccaacacc aatgtgtcct ggcagggcgg ccaaacttac accgtcaatt ggaatgtggt
      421 ggggtccgac gcttactgcc ccaatgtgga catcctgctc tccacagacg gcggccttac
      481 ttatcccact acattagcca ccgccacacc caacgacggc tcgcacgatg tcaccattcc
      541 caccatacct tccatgttga ttacggcgcg catcatggtg cggggtgcgg ggcatatttt
      601 ttatgatatt tctaatgtaa atttcacctt gctggcgggc gtgctgccgg tggagttgct
      661 ctactttgag gccgcagccg aggggaaaac agcacaattg cgttgggcca ccgccaccga
      721 gcgcaacaat gccggcttcc gcatagagcg cagcgagggg cagtcggggg gctttcaatc
      781 catcggatgg gtggcaggca aaggagatnn nnnnnnnnac ggaaaaccgc tatggcttta
      841 cggataccga agtgaagtcg gggcagacct actattatcg ccttcggcaa acggatttcg
      901 acggtgcgga aaccctgtcg gacatacaag ctgtgcgcat ggcgggagcg gcagacgata
      961 tactcgtcta tcccaatccg gcgctcgatg agttgtatgt acaatggtcg cagcccgatg
     1021 agaaaggggt gcaattggaa gtgctggatg tgcagggcag gcccctgggg ctgtgggcca
     1081 ttaccgaatc agtacatcgg gtggacacga ggcaatgggc ctccgggcaa tacattttgc
     1141 gcgttgtaac cggcggcaaa gtttggttga agcgggtgtt gattcggcgc tgacgcttct
     1201 ctttataccc gcagaatgag gctgttttct tttgggaggg cagcctcatt ttttattaaa
     1261 agatttggct aatcacaatg gagttttatc tttgtcacaa ggttttttac tgaaaaacat
     1321 tacccattga ttatcagtaa aataacctct cgccaaattg tgcttaaaaa tattttcagc
     1381 taagtattgc catgcagtac tactggaatt cttattgttt ttaagcaaac aggcgaccta
     1441 acgtgaaaaa attttaccag ccggcacaag tcggctcatt tcatcgaagc ctttctttgg
     1501 gcttttcaga tgcgaggaaa ttccttcccg gcatctgtag cttgtttttt aaatcagggc
     1561 aagctgctcc ttttctttcc gtttcggtcc aatattttac ccgtcttgat ggctttgcgt
     1621 ttgcaaaaag cgtgccgtcc ggtgggcatt ttttattgaa ataatctgag gacaaaacaa
     1681 caaatcataa tctgaccaat atggcaactt tttaccacac ggattttccc cacgaaaaaa
     1741 agacttccga ccgctccgtt caggagccgg ctcgcggtat cgaaacttgt tctaagacga
     1801 ctataaatct ctttctcatg aatcaactct ctacactcct tcccaagtgg agcgcccgtt
     1861 tcagtgcgcc cgttactttg gttttgggta tgttgttcgc tgccactact gctatggggc
     1921 agatcagcgt cacagcgacg acagcatgta ttgataaccc cggcggcacc cccgctgcac
     1981 tcaatcaata ttatgtgaga gtgacaggct taactggcgg tacatcccct tattttgttt
     2041 ccattgacgg ggtgtcgcag acatataaca cgggcgatcc tgcattggta ttcgggccat
     2101 atacccattc gggggcgggc aacgccgtgc aggttatttc ggtagttgat tctgatcctt
     2161 tattgccttt attcactact gcggaagtgg tagaagtcct ttgtggctat acgaccaata
     2221 acggtttaaa tgcatcgggt gtcagttgtg tgcctgccac ggccccctct gccaatggct
     2281 attatggtat gattctggca cagtcggcgc ccggctcttt tgcatcgggt ggcaccagcg
     2341 gccagacgca ggcatatatt ttagtgctga atgatttcat tgtacagacc aatttcacgg
     2401 gtttgttcac caatctggcg cctggcgatt atcaggttta tgccatcaac tatcgcacct
     2461 cggaggctgc tactatcaac ggctttcttc tgcctggaga agctgttcag ccgatagttg
     2521 acggcgccaa cggaacaggt cctttggtcg gagtgtgtta cacgctttgc ggcccggcat
     2581 tttatacagt cggtccgtgc tacagcgtag gcagccatgt gtttgccgat ctggatgaca
     2641 atgctatttt taaccctgga acagaaaacg ggttgcccgg cattcctgtg caacttttcg
     2701 ctcaaggcgc tgatccgact actgctactc cggtagcaag caccatcacc aatgcgtcgg
     2761 gcatatactt ctttggcggc ttgccggcgg ttagctattt catttatatt cctaccccca
     2821 atgcggccta tccatcctct tctaccaacg gcccgaacat caacagtcag acaacggata
     2881 acgatgataa cggcctgcaa actacttcag ggggcgccgt cacttccatc gtattcactt
     2941 taggtccggg tatgcctacc acagaacctg gtcccggcgg tacgcaggat gaccttgacg
     3001 atgctcgtgg caacatgacg ctcgactttg gttttgcccc cgcgccgatg attacgcact
     3061 tcaaaatgct ttcgggcatc acccaaacag ggcacaatgc ctataccgtt acttatacgg
     3121 tggaagtgcg caacaacggg gcggcaggta cataccattt gtatgataca cccaattttg
     3181 aaaacaacat caccatcacg ggcggcggat atacatccaa cttcgcagga ggtccttcgg
     3241 gatcgttcac cgcgccctac aatggtaccc aaaatcaatt agccaacaac gtatccatcg
     3301 cacttggagc tattcacatt tatacggtga gcttcaatgt gacgattgat atgctcggcg
     3361 ctaatacctt ccccaccttc gacacctaca cgcgttgcgg tggcggagtt ggcgggcctc
     3421 cggtagctgg tcaggggttg ttcaacttga gccaactgga tcgcggagca gacggcagca
     3481 ttgaggagac gcgcacagct tgtggcgacc tgccctatat tacccatgaa aaactggggc
     3541 ctgccatcag caccttgcag cctgatggtt cctacaatgt gacctatacc attcgggtgt
     3601 ataatgcagg tggcgttgcc ggcaattatg acttgtcgga tactccttat ttcgatgacg
     3661 atattaccat caacacggcc agctactcca caatgagtgt ggtgcctggc gtagtaggcg
     3721 gcgctttgtc attgaatagt ggagtactca acgcattggc caacgatcag cctattgctg
     3781 cgggtgccaa tcatacctac accttaatat ataatgtaac acttgatttg agtccgactt
     3841 ctacggatgg tggaaacaat acttatactt cttgtggcac ggtatccact gcccccggcc
     3901 cgctgacgcc cggcagtacg gtaactaccc ggatgggtct gtataatact tctgcgttgg
     3961 ataccaacaa tgatgccaac ccggatgaag tggacgaaag atgtggcgac ctgccgttcc
     4021 tgtcgcttgc caagacattc gtgtcagcgg tacctacagc ggcagcgggt gcttacacag
     4081 tgacttatac gatcacggtc atgaacactg gaggtgcgac aacaacttat gacctgcgcg
     4141 atgtaccggg attcgatgat gatatagcta ttacgagtgc gtcttatgcg ggagtaggtg
     4201 tgggtgcgcc ggccggcgcc gccttggcgg gcagcggccc gtggttgctg gcagacgacg
     4261 caagcattgc cgcaggagtg acgcatacct atactttgat ggtgaatgta ttgcttgatc
     4321 tgtcggctgg ttcttccggc aacaattcat acacagcttg cggtacgacg cagggatcaa
     4381 cctctccgat tcc
//
LOCUS       DAORLF010000411         4382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10927_length_4382_cov_1.593252, whole genome shotgun sequence.
ACCESSION   DAORLF010000411 DAORLF010000000
VERSION     DAORLF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10927_length_4382_cov_1.593252"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1466)
                     /locus_tag="PK198_22760"
     CDS             complement(<1..>1466)
                     /locus_tag="PK198_22760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF41641.1"
                     /translation="QKIATANPLQALQGKVAGVQVSAASGRPGEGPVVRIRGTGTLNG
                     AAPIYVVDGLILNNIDFLNAADIESMEILKDASAAAIYGTRGANGVILISTRKGSRSQ
                     KARVSFGSYVGQQQPERRLGLANATEYAQLINEIALNNGSQPVHPDPTVFGVGTDWQD
                     VIFQNGIIQNYNLNVSGGSEAMTYSISGDVFRQEGIVKSSYFNRYTLRVNTQYNFFKW
                     MKFGHNIAFVMSDNNGEPGGIVFNAIASDPTVIPRDENGRFGSTSLVSNVSNPAAQLE
                     YNSYNRGYGQQFAGNLYGDIFILKDLTFRSSFGFNLVNNRNKSYEPVFEVDNKQRNPE
                     SRIFAGFYRGVDWQWENTLNYRKEWKNHRIELLAGYTSQNITGEGIGGGRLRLIGTTE
                     NFFFLNAGDDQTATNYHYANRPERYVSTLFRLNYSLMGKYLLTASWRRDGSSRFGSER
                     RFGSFPSVALAWRVIEEPFMQDQKILTNLKLRASWGRL"
     assembly_gap    1467..1535
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1536..>1857)
                     /locus_tag="PK198_22765"
     CDS             complement(<1536..>1857)
                     /locus_tag="PK198_22765"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41642.1"
                     /translation="MIGKQLLISAALMLWSVWAVAQVTVTGKVTDDKGEALIGANILV
                     KNSTNGTITDIDGSYELKVPDAFVTLVVSYTGYTTQEFPLKGQTKLDVVLLEQATLIS
                     EVVVV"
     assembly_gap    1858..2071
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2088..>2402)
                     /locus_tag="PK198_22770"
     CDS             complement(2088..>2402)
                     /locus_tag="PK198_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HRF41643.1"
                     /translation="FDRNESVNISVMLKNTGNYDGEETAQLYVRDLIGSVTRPVRELK
                     GFQKVFLKKGESKTVQFRLSADDLRFYNHDLRFRAEPGDFDIWVGGDSDAKLGTRVTM
                     VK"
     assembly_gap    2403..2421
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2422..4152)
                     /gene="bglX"
                     /locus_tag="PK198_22775"
     CDS             complement(<2422..4152)
                     /gene="bglX"
                     /locus_tag="PK198_22775"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRF41644.1"
                     /translation="MNAIVLYKEDIKKGRVGAIFNAHTAAYTKELQRIAVEETRLGIP
                     LLFGYDVIHGYKTIFPIPLGESASWDLQAIEKSARIAGVEAAASGLHWTFAPMVDIAR
                     DPRWGRMAEGAGEDTYLGSAIAKARVQGFQGNGFDNADAVLACAKHFAAYGAAQAGRD
                     YHTVDISEQVLREVYLPPFKAAVDAGAWTFMTSFNEWNGIPASAHEQLLKDILKWEWG
                     FPGFVVTDYTSINEMVQHGYAADFKHAGELSVNAGVDMDMQGAVYYNHLEQLIREGKV
                     KESRVDDAVRRILRMKFMLGLFENPYKFSDTEREKRLILSQEHLAAARDVARKSIVLL
                     RNENQLLPLSKNLKNIAVIGPLADSKVDMLGNWSAAGDAKDCVTLLEGVRAKWPNAQI
                     KYARGSDVMQMNKVDFANAYAAAKKADVIILAVGEAGWMSGEAASRSDIGLPGVQTEL
                     VKYLLTTGKPVVMVLMNGRPLVLGDLAEKVPAIVETWFAGTQGGHAIADVLAGDYNPS
                     GKLPVTFPRNVGQIPLHYNMKNTGRPMNPNDKYTSKYLDVPNTPQWPFGFGLSYTTFE
                     YSDLKTDRSSF"
     gene            complement(4109..>4382)
                     /locus_tag="PK198_22780"
     CDS             complement(4109..>4382)
                     /locus_tag="PK198_22780"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF41645.1"
                     /translation="SKLGMPVMLKAAHGGGGRGMRVVKTEGELEGKLLEAKRESLTAF
                     GKDDVFIEKYVEHARHIEVQILGDQHKNIVHMFERDCSLQRRHQKG"
BASE COUNT          879 a         1117 c         1098 g          986 t
ORIGIN      
        1 caaacggccc caactggcgc gcaatttcag gttggtcagg attttctgat cctgcatgaa
       61 aggctcctca atgacccgcc aggccaatgc caccgagggg aaactgccga aacgacgctc
      121 ggaaccaaaa cgggaggaac cgtcgcggcg ccaggaggca gtgagcagat atttgcccat
      181 taaagagtag ttgagcctga acaaagtaga aacatagcgt tccgggcggt tggcatagtg
      241 gtaattggtg gcggtttgat cgtcgccggc gttgaggaag aaaaagttct ccgtagtgcc
      301 gatgagcctt aaacggccgc cgccaatgcc ctcgccggtg atgttttggg aagtataacc
      361 ggccaggagt tcaatgcggt ggtttttcca ctctttgcgg tagttgaggg tgttttccca
      421 ctgccagtcc acaccccggt agaagcctgc gaaaatgcga ctttcggggt tgcgctgctt
      481 gttgtccacc tcaaaaacag gctcgtagct tttgttgcgg ttgtttacca aattgaagcc
      541 gaaactgctg cggaaggtaa ggtccttgag aataaaaata tcgccgtaga gattgccggc
      601 aaactgctgc ccgtagcccc ggttgtagct gttgtactcc aactgggcag ccggattgct
      661 cacattggac accaacgagg tgctgccgaa acggccgttt tcatcgcgag gaatcaccgt
      721 cggatcggaa gcaatggcgt tgaacacaat gccgccgggt tcgccgttgt tgtcgctcat
      781 cacaaaggcg atgttgtgcc caaacttcat ccatttgaag aagttgtact gggtgttgac
      841 ccgcaaagtg tagcgattga aatacgacga cttcacgatg ccctcctgcc ggaacacgtc
      901 gccgctgatg ctgtacgtca tggcttcgga gccgccgctg acattgaggt tgtagttttg
      961 gatgatgccg ttttggaaaa tgacatcctg ccagtcggtg cccacaccaa acacggtagg
     1021 atcggggtga acgggctggc tgccgttgtt gagggcaatt tcgttgatga gctgcgcgta
     1081 ttcggtggca ttggccaaac ccagacggcg ttcgggctgc tgttggccca cataagagcc
     1141 aaacgaaacg cgggcttttt gcgagcggga gcctttgcgc gtgctgataa ggataacgcc
     1201 gttggcgccc cgtgtaccgt aaatggccgc agcggaggcg tctttcaata tctccatgct
     1261 ttcgatatcg gccgcattaa gaaagtctat gttattgaga atcaggccat ccaccacata
     1321 aataggggcg gcgccgttga gggtgcccgt accgcgaatg cgcaccacag gaccttcgcc
     1381 cggcctgccg gaagcagcgc tgacctgcac accggccact ttgccctgca atgcctgcaa
     1441 ggggttggcg gtggcaattt tctgaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacta ccaccacctc gctgatgagg
     1561 gtggcctgtt cgaggagtac aacgtccaac ttcgtctggc ctttgagcgg gaattcctgc
     1621 gtcgtgtagc ctgtgtaaga taccaccagc gtgacgaaag catccggcac tttcagctcg
     1681 taagagccgt ctatgtcggt gatggttccg ttggtggagt ttttcacaag gatgttggcg
     1741 ccgatgagcg cttccccttt gtcatcggtc acttttccgg tcaccgtaac ttgagcgacg
     1801 gcccatacgc tccatagcat cagtgccgcc gaaatgagta gttgtttacc aatcattnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacccctga aaaccgccta cttcaccatc
     2101 gtcactctcg tacccaattt cgcatccgaa tcgccaccca cccaaatatc gaagtcgccg
     2161 ggttcggcac ggaagcgcaa gtcgtggttg taaaaacgca agtcatcggc gctcagccgg
     2221 aattgcacag ttttgctttc accttttttc aaaaacacct tttggaagcc cttcaactcc
     2281 cgcacaggcc gggtcacgct gccgatcaga tcgcgcacat agagctgcgc ggtttcttcg
     2341 ccgtcgtaat tgcctgtgtt tttgagcatt acagaaatat tcaccgattc gttgcggtca
     2401 aannnnnnnn nnnnnnnnnn naaagctcga ccggtctgtt ttcaggtcgc tgtactcaaa
     2461 agtagtgtag ctcaacccga atccgaaggg ccattgcggc gtattgggta catctaagta
     2521 cttgctggtg tacttgtcgt tcggattcat ggggcggccg gtgtttttca tgttgtagtg
     2581 caagggaatt tgccccacat tgcgcggaaa ggtcaccggc agtttcccgg atgggttgta
     2641 atcgccggcc agcacgtcgg caatggcgtg accgccctgc gttcccgcaa accatgtttc
     2701 cacgatggcc ggtacttttt cggccaaatc tcccaatact aaaggacggc cattcatcag
     2761 caccatcacc actggttttc cggtggtcaa caggtatttt acgagttctg tctgcacgcc
     2821 cggcaatccg atatcagaac gactggccgc ttcgcccgac atccagccgg cctcgcccac
     2881 cgccaaaatg atgacgtcgg cttttttggc ggcagcataa gcattggcaa aatcaacttt
     2941 attcatctgc atcacgtcgg aacctctggc gtattttatc tgcgcattgg gccatttggc
     3001 gcgcacgcct tccagcagcg tcacacagtc tttggcatcg ccggcagccg accagttgcc
     3061 cagcatgtcc actttgctgt cggccaacgg gcctatcacg gctatgtttt tgaggttttt
     3121 ggacagcgga agcaactgat tttcattgcg cagcaacaca atggacttgc gggccacatc
     3181 gcgggcggcg gctaagtgct cctgcgaaag gatgagccgt ttttcacgct cggtatcgct
     3241 gaatttgtag gggttttcaa acaggccgag catgaatttc atgcgcaaaa tgcgacgcac
     3301 ggcatcgtcc actctcgatt ctttcacctt gccttcgcgg atcagttgtt ccaaatggtt
     3361 gtaataaacc gcgccctgca tgtccatgtc cacgccggca ttcaccgaca gttcgccggc
     3421 gtgtttgaag tcggcggcat aaccgtgctg caccatctcg ttgatggagg tgtagtcggt
     3481 caccacaaat ccggggaaac cccactccca tttgaggatg tctttgagca gttgttcatg
     3541 ggcgctggcg ggtatgccgt tccattcatt gaaggaggtc atgaaggtcc aggcgccggc
     3601 atctacagca gccttgaaag gcggcagata tacctcacgc agcacttgct cgctgatgtc
     3661 cacggtgtga tagtcgcgac cggcctgagc ggcgccatac gcggcaaagt gtttggcgca
     3721 agccagtact gcatcggcat tgtcaaaccc attgccctga aagccctgca ccctggcttt
     3781 ggcaatggcc gaaccgaggt aagtgtcctc tccggcaccc tcagccatgc ggccccagcg
     3841 cggatcgcgg gcaatgtcca ccatcggcgc gaaggtccaa tgcaagccag atgcggctgc
     3901 ttccacgcct gctatgcgcg ccgatttttc gatggcttgc aaatcccaac tggccgactc
     3961 gcccagcggt ataggaaaga tggttttgta gccatgaata acgtcgtagc cgaaaagcaa
     4021 ggggatgccg aggcgggtct cctccactgc aatgcgttgt aattctttgg tataggcagc
     4081 ggtatgggcg ttgaagatgg cccctaccct accctttttg atgtcttctt tgtaaagaac
     4141 aatcgcgttc aaacatatgc acgatatttt tatgttgatc gcctaaaatt tgtacttcaa
     4201 tatggcgagc atgttctaca tatttttcaa tgaaaacatc atctttgcca aaggcggtaa
     4261 gcgattcgcg ttttgcctca agaagtttac cttctagttc gccttctgtt tttactacgc
     4321 gcataccacg gccaccacca ccgtgtgcgg cttttagcat aacgggcata cctagttttg
     4381 ag
//
LOCUS       DAORLF010000412         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10975_length_4367_cov_2.425093, whole genome shotgun sequence.
ACCESSION   DAORLF010000412 DAORLF010000000
VERSION     DAORLF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_4367_cov_2.425093"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(474..2063)
                     /locus_tag="PK198_22785"
     CDS             complement(474..2063)
                     /locus_tag="PK198_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF41646.1"
                     /translation="MKKASFLALLFIGIACQNFLAAQSRATAPDPVLIASGITASELQ
                     RHLTVLASDEMQGRETGTEGQRKAAQYIADVFSSWQLPAVGDDNSYFQHILFKAESWK
                     RVELTIGDKKYRHLWEYYAAPHLNEGSASPMSFNEVLFLGYGIDDANYSDYKGVDVKG
                     KALLIYDGEPVNASGISYVTGTSERSNWTTEQKLAVAKSKGAALVLIIDSSFKENVAE
                     VRKALSNTRLQPAKSRLNLRNLAPNCHLSSETAKALLGTQVKKVIRQRDKILKKGRPS
                     DAVIPAKIEVTLDKETRELSGHNVLGYVQGSDPVLQNELVIVSAHFDHLGMRGDAIYY
                     GADDNASGTSAVLEIAQAFSDAKQAGMGPRRSVLFLLVSGEEKGLLGSQYYVEHPTFP
                     LANTIANVNVDMVGRVDAKHADNPHYIYVIGADRLSTELHRINETANATYTQLELDYT
                     YNAKNDPNRYYYRSDHYNFAERGIPAVFYFSGTHQDYHRTTDTVDKINFDKMVKIAQL
                     VFHTTWELANRDQRIQVDVKK"
     gene            complement(2136..2579)
                     /gene="rpiB"
                     /locus_tag="PK198_22790"
     CDS             complement(2136..2579)
                     /gene="rpiB"
                     /locus_tag="PK198_22790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRF41647.1"
                     /translation="MSVQRIAIGCDHAGFPYKRAVVLLLESLGMEVLDFGAHSEDSVD
                     YPDFVHPVALSVEAGDANLGIVMCGSGNGVAMTANKHQGIRAALCWTEELAALARQHN
                     NANVLALPVRFITETQALAIVKSFLEAAFEGGRHQRRVDKIACGS"
     assembly_gap    2582..2611
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2612..>4367)
                     /locus_tag="PK198_22795"
     CDS             complement(<2612..>4367)
                     /locus_tag="PK198_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRF41648.1"
                     /translation="TQGYFPSDKANGHWLSGIDALDNGRLYDQSKITQTMREDKSRNR
                     YYMIPFFLGLIGLFYHLLRRPQEGLALLAMFIITGIGIIIYSNQPPNEPRERDYVLVG
                     SFFTFCIWIGMAAPAIYDLISQKAKLPGIIGGALSLAVLSAPWLMGTQNFDDHSRKGH
                     TAARDYASNFLNSCEKDAVIFTFGDNDTYPLWYAQEVEGIRTDVRVVNLSLIAVDWYI
                     DLLRRRVGESAPLKFTVPADKVRGSRRNQIPYYNPSRGADQPMSAEDWLRFIALDNPL
                     PTTSGRGFDAYYPTKQIYLPIDREKAIKAGMVTEKDTAVAEFIPLRQPGDYITKDDLA
                     ILDIIVSNMYDRPIYFAVTCQPSKFWGLNEYMQLEGLALRIIPVRSESERSYSVVGSG
                     RVDSEKVYKNVMEKFRWGNFDQLDVHVNTSYQPSLQTMLFAMRRAAINFIEIGQPQKA
                     VELMDKYFEVFPHKNFPYDYRSWYMISVYLAAGAYDKAKPHMEILAKETADALAFYES
                     LDPVVLSSSQEFDDRYSLCYRTKGDLLNAARKEKDEAFTKQLEEMFAPFKADEQIPQE
                     LPPSIQQQAPQPAPPGTSV"
BASE COUNT         1012 a         1103 c         1114 g         1108 t
ORIGIN      
        1 tccttactcc agattgctta tcctgagctt ctttaaaagc tcaaaatctt ctaaaaggtc
       61 tcacactcaa ttcttaccga gttttctaat gtcttacctt cttgctacag tgtcaatgaa
      121 ctttttgacg cctcgcctcc atgttcagta cccgttggcg ctttgagagc gaattgtcat
      181 atctcttcaa gctgacgctt gggttgatct tgcaaaactt accgcttttc ggtaagggac
      241 ggcaaaggta gaaatgattt tcaaatccat atacacattg cccaaaaaaa tcttttcttt
      301 ttttatcgag gcgccattac gtctcgactg cttttcaaaa gcggacgcaa aggtaagtcc
      361 gctttttcaa tctgtcaagc atttttaaga aaaaaaatca tcaaataatc ggctttggaa
      421 aacacgggca attttagccc tgacaatcag catctaatga aatgctcgtg aaattatttt
      481 tttacatcca cctgtatgcg ctgatcgcga ttggccaatt cccaagtcgt atggaacacc
      541 aactgagcaa ttttgaccat tttatcgaag ttgatcttat ctactgtatc ggtagttcgg
      601 tggtaatcct gatgggtacc gctgaaataa aagacagcgg gaatgcctcg ctcggcaaaa
      661 ttgtagtgat ctgagcggta gtagtagcgg ttggggtcat ttttagcatt ataagtgtaa
      721 tccaattcca actgtgtgta ggttgcattg gccgtttcgt tgatgcggtg caactctgta
      781 ctaagcctgt cggcgccgat gacatatata taatgtgggt tgtcggcatg tttggcatcc
      841 acccgcccca ccatatctac gttcacattg gctatcgtgt tggccagagg gaaggtagga
      901 tgctctacat agtactggct ccccaagaga cctttttcct cgccggaaac gagcaaaaac
      961 agcacacttc tacgcggccc catgccggct tgcttcgcgt ctgaaaatgc ctgcgcaatc
     1021 tccagcaccg ccgaagtacc cgacgcatta tcatcagcac catagtatat agcatcgcca
     1081 cgcataccga ggtggtcaaa atgggcagaa actatgacca attcgttttg aagcacaggg
     1141 tcggacccct gcacatagcc caaaacattg tgtcccgaca attcgcgggt ttctttatct
     1201 aaggtcactt ctattttggc aggaatgacg gcatctgaag gacggccctt ttttagaatt
     1261 ttatcccgct gccggatcac ctttttcact tgcgttccca gcaatgcttt ggcggtttct
     1321 gaagataaat ggcagttggg cgccaaattc ctgagattga gcctgctttt ggcgggctga
     1381 agccgggtgt tgctgagcgc tttgcgaacc tccgctacgt tctctttaaa cgaggaatca
     1441 ataattaaga cgagcgccgc ccccttcgat ttggcgacag ccagcttttg ttctgtggtc
     1501 cagttggaac gctccgaggt gcccgtgaca tacgaaatcc cggaagcatt caccggttcc
     1561 ccatcgtaaa tcaacaaggc tttgcccttc acatccacac ctttgtaatc gctgtaattc
     1621 gcatcgtcaa taccgtatcc caaaaaaagt acttcattga aagacatcgg tgaagcgctg
     1681 ccttcattca aatggggtgc agcgtagtat tcccaaaggt gcctgtattt tttgtctcca
     1741 atggtaagct ccacgcgttt ccagctttcc gctttgaaaa ggatgtgttg aaaataggag
     1801 ttgtcgtcgc cgacagcagg cagttgccag gaggaaaaca catctgcaat gtattgagcg
     1861 gccttgcgct gtccttctgt gccggtttcg cggccttgca tttcatcaga agccagcacg
     1921 gtgaggtgtc gctgcaattc cgacgctgtg atgccggacg caatgagcac aggatcgggc
     1981 gcagtggcgc gcgattgtgc agcaaggaag ttttgacagg ctatgccgat gaacagcaag
     2041 gccaggaatg atgccttttt catagtaata caggtatgtg gaatcgccag atatgaatga
     2101 tctgacgttc gattcgtaaa gccgctattt tcgtttcaac tgccgcaggc aattttgtcc
     2161 acccgccgct gatgtctccc gccttcaaac gctgcctcca agaaagactt gacgatggcc
     2221 agcgcttgtg tctctgtaat gaacctcacc ggcagggcca acacattggc attgttgtgt
     2281 tgtcgggcca gggcagccag ttcttcagtc caacacaaag cggccctgat gccctgatgt
     2341 ttattggccg tcatggctac gccgttgccg ctgccgcaca taacgatccc cagattggca
     2401 tctccagcct ctacgctgag cgccaccgga tgtacaaaat cggggtaatc aaccgaatcc
     2461 tcggaatggg cgccaaaatc cagcacttcc attccgagag actccagcag cagaacgacg
     2521 gctcttttgt aggggaagcc ggcgtgatcg caaccgatgg ctatgcgttg tacagacatc
     2581 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacagaagt acccggcggc gccggctgag
     2641 gcgcttgctg ttgaatggaa ggcggcagtt cctgcggaat ttgctcatcg gccttgaaag
     2701 gcgcaaacat ctcttccaat tgcttggtaa acgcctcgtc tttttctttg cgggcggcat
     2761 tcaacaggtc tcctttggta cgataacaaa gactgtaccg gtcgtcgaat tcctggctgg
     2821 acgacaacac taccgggtcc agagattcat agaatgcgag cgcatcggca gtttcctttg
     2881 ccaaaatctc catatgaggc ttggctttgt cgtaagcgcc ggcagccaga tacacggaaa
     2941 tcatgtacca ggagcggtag tcgtacggga agtttttgtg cgggaaaacc tcgaagtact
     3001 tgtccatcag ttctacggcc ttttgaggtt gaccgatttc gatgaagttg atggcggctc
     3061 tgcgcatggc aaacagcatg gtttgcaggc tgggctggta gctggtattg acgtgtacat
     3121 caagctggtc gaagttgccc cagcggaatt tctccatcac gtttttatat accttttccg
     3181 agtccacccg accgctaccg accacgctgt agctgcgttc gctctcgctt ctgacgggga
     3241 taatgcgcaa tgccagcccc tccaattgca tgtattcatt cagcccccag aacttggacg
     3301 gctggcaggt cacggcgaaa taaataggcc ggtcgtacat attggaaacg atgatatcca
     3361 aaatggcgag atcgtctttg gtgatgtaat cgcccggctg acggaggggg ataaattccg
     3421 ccacagcggt atctttttct gtaaccattc ctgcctttat ggccttttcc cggtctatcg
     3481 gcaaatagat ttgcttggta ggataatacg catcgaagcc ccggccggag gtagtaggca
     3541 gcgggttgtc caacgcaatg aagcgcagcc agtcttcggc gctcatcggt tggtcggcgc
     3601 cacgagacgg attgtagtat ggaatctgat ttcggcggct gccccttact ttgtcggcag
     3661 gaacggtaaa tttgagcgga gcagactctc ccaccctgcg gcgcagcagg tcaatgtacc
     3721 agtccaccgc aatgaggctg aggttgacca cgcgcacatc ggtgcgaatg ccctctactt
     3781 cctgcgcata ccacagcggg taggtgtcgt tgtcgccgaa agtgaaaatg acggcatctt
     3841 tttcgcagga gttgaggaaa ttcgacgcat aatcgcgggc ggcggtatgg cccttgcggg
     3901 aatggtcgtc gaaattttgg gtacccatca gccagggcgc cgacagcacg gcgaggctga
     3961 gcgcgccgcc gatgattccc ggcagtttgg ccttttggct gatcaaatcg taaatagccg
     4021 gcgcggccat tcctatccag atacagaaag taaagaacga accgacgagt acataatcgc
     4081 gctcgcgggg ttcgttgggc ggctggttgg aataaataat gatgccgata cctgtgatga
     4141 tgaacatggc cagcaatgcc aatccttcct gcgggcgacg gagcagatgg tagaacaacc
     4201 cgatcaggcc cagaaagaag ggaatcatat agtagcggtt gcgggatttg tcttcccgca
     4261 tcgtttgggt aattttgctc tgatcgtaca ggcggccatt gtcgagggca tctatgccgg
     4321 agagccagtg cccgttggct ttatcgctgg ggaagtagcc ctgcgta
//
LOCUS       DAORLF010000413         4361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10990_length_4361_cov_2.152578, whole genome shotgun sequence.
ACCESSION   DAORLF010000413 DAORLF010000000
VERSION     DAORLF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10990_length_4361_cov_2.152578"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="dapA"
                     /locus_tag="PK198_22800"
     CDS             complement(<1..777)
                     /gene="dapA"
                     /locus_tag="PK198_22800"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRF41649.1"
                     /translation="MNPLSKGGTGVALITPFLRNDVDFPALENIIEHVITGGADFIVS
                     LGTTGEAITLSPDEVNEVLQFTIKKVAGRIPVVAGFFGDNYTDRLAQKLRSYDFTGVA
                     AVMSSSPSYNKPTQEGIYRHFMAVAEASPLPIIIYNVPGRTAANMNAETTLRLAEASE
                     RFLAVKEASGVMAQSMKILRDKPAHFQLWSGDDVLTLPLIACGATGVISVIANAWPAE
                     FSGMVRAARSGNWPEARRLNDLLLNIHPHLYTEGNPGGIKA"
     gene            complement(774..1313)
                     /locus_tag="PK198_22805"
     CDS             complement(774..1313)
                     /locus_tag="PK198_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41650.1"
                     /translation="MGLLHDFKTGLQKRALRRVMAQPSSASRGAMHYDHARQVSILFN
                     ASELKEREQVLRFAEKLSQSGKKVSLLGFFDHPVEFSDFVFPGFTADDFDWRLQPKSE
                     AVNHFLQQPTDLLIHANTRPDMYELFVAASSKAKVRVGPYADYTECYELMADTSGNTD
                     IVYYLQQVEFLLQKTNTKP"
     gene            1526..>1899
                     /locus_tag="PK198_22810"
     CDS             1526..>1899
                     /locus_tag="PK198_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF41651.1"
                     /translation="MNTKIATALDNASMQRDLIEQCKAGNRTAQFELYKQYSKGMYNV
                     CLRMVHDSMDAEDVLQNAFVDIFTKMDSFRYESTIGAWIKRIVVNHCINFLKKKKLQW
                     EELDDRFVNLREDQGPETNDSRL"
     assembly_gap    1900..1917
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1918..2113
                     /locus_tag="PK198_22815"
     CDS             <1918..2113
                     /locus_tag="PK198_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HRF41652.1"
                     /translation="EQINKAIMMLPDGYRVVFSLYLLEGYDHEEISEILQISEATSKS
                     QFSRAKAKLRDILSNKREIN"
     assembly_gap    2247..2338
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2339..2707
                     /locus_tag="PK198_22820"
     CDS             <2339..2707
                     /locus_tag="PK198_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41653.1"
                     /translation="MAGLGLAGRQQSSVMAEIEQVNPDFREAEKYYNEQIDSKIAQLA
                     SYKTEAAPVLEDLAQVDEMMQELRQELASAPAGAEEQIVSDLIVSYRTKLAILEKVLE
                     SISNKDAYQKSKTQQNETGI"
     gene            2691..>3242
                     /locus_tag="PK198_22825"
     CDS             2691..>3242
                     /locus_tag="PK198_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41654.1"
                     /translation="MKLAFKTSLLFLATLISATPLIGDPCDEAFREFTKTIKKEYDIS
                     SEGTVHLSNRYGKMEVKTWDKNRVKIEVTILVNAGSESQAQEVFDRISINFSNSQAYV
                     KAETVIESASKSWWNWFNNNTADYKINYLVYMPPTANLELYNKYGDAYIAELKNRALI
                     NVKYGNFKADGVGDKTTVELAYGN"
     assembly_gap    3243..3302
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3303..>3567
                     /locus_tag="PK198_22830"
     CDS             <3303..>3567
                     /locus_tag="PK198_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41655.1"
                     /translation="NLTCTELKDADLTSKYSKTYIERAGVIRSTSKYDEYKIGKVREF
                     RNSGGYDNFDIRSVENAVLSSKYSNANIGWLGNSIDFTVSYGGL"
     assembly_gap    3568..3669
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1099 a         1007 c         1051 g          932 t
ORIGIN      
        1 cgccttgatg ccgccgggat tgccctcggt gtagaggtgc gggtgaatgt tcaagagcag
       61 gtcgttgagg cgccgcgctt ccggccagtt gcccgaacgg gcagctcgca ccatacccga
      121 aaactcggca ggccaggcat tggcaataac ggaaatgacg cccgtggctc cgcaggcaat
      181 gagcggcagg gtaagcacat cgtcgcccga ccaaagctgg aaatgagccg gtttgtcgcg
      241 cagtattttc atggattgcg ccataacgcc cgatgcctct ttcaccgcca aaaaacgttc
      301 ggatgcctcg gcaagccgca gcgtggtttc ggcattcata ttggcggcgg tgcggcccgg
      361 cacattatat ataatgatgg gcaagggact ggcctcggcc acggccataa agtgccggta
      421 gatgccttcc tgcgtgggtt tgttgtagga cgggctgctg gacataacgg cggccacgcc
      481 cgtaaaatcg tagctgcgca atttttgagc cagacgatct gtgtagttgt cgccgaaaaa
      541 gccggctacc acgggtatgc gcccggccac tttttttatg gtaaactgca agacttcatt
      601 cacctcgtcg gggctgaggg tgatggcttc gccggtagtg ccgaggctga cgatgaagtc
      661 tgcaccgcct gtaatgacgt gttcgatgat gttttccagc gcaggaaaat caacatcatt
      721 gcggaggaag ggcgtaatga gtgctacgcc ggtaccgcct ttgctgagtg gattcatggt
      781 ttggtgttgg tcttttggag caaaaattcc acttgttgga ggtaataaac gatgtcggta
      841 ttgcccgaag tgtcggccat gagttcgtag cactctgtat agtcggcgta aggaccaacc
      901 cgtaccttgg cttttgagga cgccgctacg aacagttcgt acatatcggg gcgggtattg
      961 gcgtggatga gcaggtccgt cggctgttgg agaaaatggt tgacggcctc ggatttaggt
     1021 tggagcctcc agtcgaaatc gtcggctgta aaaccgggaa acacaaaatc cgaaaactcc
     1081 accgggtgat cgaaaaagcc cagcaaactc acctttttac cgctttggct cagcttttct
     1141 gcaaaccgga gtacctgttc ccgctctttt agttccgagg cattgaataa gatactcacc
     1201 tgccgggcat ggtcatagtg catcgcgccg cgggaggccg aagatggttg tgccatgacg
     1261 cgccgcaaag cccgtttttg caggccggtt ttaaaatcgt gcagtaatcc catgtagctt
     1321 cgtttatcgg cgcgcaaagg tacatacttt tcaggttatg gctgagatat acaagaacag
     1381 gggtgtttca gacacctccc cacctttttt acaaaaaaac gcccctccat gcaacccttc
     1441 cacagggcgt attgtccttc ttaacaaata ttcggaagaa aacccgatac acaccatcgg
     1501 gcagccgcac aaagcaggca actgaatgaa cacaaaaatc gcaacggcct tggacaacgc
     1561 cagtatgcaa cgggacctga ttgagcaatg taaagccggc aaccggactg cacagttcga
     1621 gttgtacaaa caatattcca aaggcatgta caatgtgtgt ttgcgcatgg tgcatgattc
     1681 tatggatgca gaggacgtgt tacagaatgc gttcgtggat atttttacaa aaatggacag
     1741 tttccgctac gagtccacca tcggagcctg gatcaagcgc atcgtcgtca atcattgcat
     1801 caattttctg aaaaagaaaa aattgcaatg ggaagaattg gacgaccgct tcgtcaacct
     1861 ccgcgaagac caggggccgg aaaccaacga tagccgcctn nnnnnnnnnn nnnnnnngga
     1921 gcagatcaat aaagccatta tgatgctccc cgacggctac cgggtggtgt tttcgctgta
     1981 cttgctggaa gggtacgacc acgaggaaat ttccgaaatc ctccaaattt cggaagccac
     2041 ctccaagtcg cagttcagcc gggccaaagc caaactgaga gatattttgt ctaataagag
     2101 ggaaatcaat tgattatgaa acacgacagc ttagagcaat tcattcggga acaccgtacc
     2161 gaattcgacg ccgaacatcc gccattgcat atttgggcgg gcatcgaaag gcaattgggc
     2221 acagaaaaaa aacctgaaag tgccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     2341 ggccggactc ggcttggccg gccgacagca atcttcggtc atggccgaaa ttgagcaggt
     2401 caacccggat tttagggaag ccgaaaaata ctacaatgaa cagattgatt ccaaaatagc
     2461 tcagttggca tcttacaaaa cggaagccgc gcctgtgctg gaagacttgg cccaggtgga
     2521 cgagatgatg caggaactca gacaagaact ggcttcagcg cctgccggag ccgaggagca
     2581 aattgtttcc gatctcatcg tcagctaccg caccaaattg gccattttgg aaaaagtcct
     2641 cgaaagtatt tcaaacaaag acgcatatca gaagtcaaaa acgcagcaaa atgaaactgg
     2701 catttaaaac atcactcctc tttttggcaa cgcttatcag cgcaacgccc ctcatcggcg
     2761 atccctgtga cgaagcattc cgggagttta ccaaaaccat caaaaaagag tacgacatta
     2821 gctcggaggg caccgttcat ttgagcaaca ggtacggcaa aatggaggta aaaacctggg
     2881 ataaaaaccg cgtaaaaatt gaggtaacga tccttgtcaa cgcaggctcg gagtctcagg
     2941 cacaggaggt ttttgatcgc atcagcatca atttctccaa ctcacaggct tacgtcaaag
     3001 cggaaaccgt cattgagtcg gcctccaaat cctggtggaa ctggttcaac aacaacaccg
     3061 ccgactacaa gatcaattat ttggtctata tgccgcccac cgctaatctg gaactctaca
     3121 acaagtatgg cgacgcctac attgccgaac tgaaaaaccg ggcgctcatc aatgtcaagt
     3181 acggcaattt caaagccgac ggcgtgggcg ataaaactac ggtggaactg gcttacggca
     3241 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnaacctgac ttgtaccgaa ctgaaagatg ccgacctgac gtcgaaatac tccaaaactt
     3361 acattgaacg cgcaggcgtc attcgcagta cctccaaata cgatgagtac aaaataggca
     3421 aagtgcggga atttcgcaat tcgggcggct acgacaactt cgacatccgc tccgttgaaa
     3481 acgcggtgct ctccagcaaa tactccaacg ccaatatcgg ttggctgggc aactccattg
     3541 acttcaccgt atcttatggc ggcttggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnna tttgaagctg tgggcagcta tacgggcatt acctacccct ccggcatgtc
     3721 ggtttcttat gaaaaagaac agggccaaag ccacgaagtg aaagggcaac tcggagcttc
     3781 gggcaaagga tacatcaaag cgcagttgag ctacggcaat atcaagctgc ggcaagaata
     3841 gggcgcaaaa acaaatgttt attttcgcca aaggatcagt taagtaaacg cttcatttta
     3901 agtgccttat ctgatccttt ggccggaaga aaatgaacat tgtttttgcc cggtactaag
     3961 gacacatttc atactaattc gagtttttgg cagaagcgct tcattcgcgg caggcgggtg
     4021 taagcgtttc tctatttgga acgagcccct ctgcttcgcg gtcttcgcgg gaggaaaagt
     4081 gtgtacaaca cattttaacg aacaaagcct atgctgaaat tctgaaatat gaaccgcgtc
     4141 ctatttttga cgccgatctt ttatgattgg taggatttgc gctgctcgta gagggcataa
     4201 caatcataaa aatctgcttc tttctgcgtc tgaagctgtg cctcgtttat tctttagaca
     4261 ggcttcgacg ggtggcggag cgaaaatttg acaaatcttc gagatttgtc aaattgtgtg
     4321 ctgcctgaaa accgcgcctg attttgtgca attacacacg g
//
LOCUS       DAORLF010000414         4360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_10992_length_4360_cov_1.942625, whole genome shotgun sequence.
ACCESSION   DAORLF010000414 DAORLF010000000
VERSION     DAORLF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10992_length_4360_cov_1.942625"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK198_22835"
     CDS             complement(<1..759)
                     /locus_tag="PK198_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41656.1"
                     /translation="MTDINTPFNEGAQSISADGRTLVFNACNLPEGLGSCDLYISYFI
                     HGRWSRPNNLGDPANSKYLERQPTLSADGKVIIFSSDRPGGYGGRDLWLVRRKPNGDW
                     SLPANLGATINTAGNEDCPFFHPDGQTLYFMSDGHPGMGGFDLFLSRLSPEGIFGKPQ
                     NLGYPINTTANEGALIVSLDGATAWFTSDAGDPDAKPGDNPAGINTDILQFEMPPGLR
                     PAPVTYVKATVRDAVSGQLLPARSELHLMPENRLL"
     gene            743..1285
                     /locus_tag="PK198_22840"
     CDS             743..1285
                     /locus_tag="PK198_22840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41657.1"
                     /translation="MLISVMGWAFDAVGRFPAEVKVFLPVDDARKHQRIAVGGDRGQA
                     FGHGRIDTVAQWFGLKGQRIFHGFGGKAGIGQIAFGALFQVLVSARRLQKPLKSRRRR
                     IVFFQTQPAHSQRIPGFGVVIGGQCERRFKQFFRLGIVFLIVANGGFQQLRVNEPGRF
                     PQGGLCLFEGFGVAACLAGG"
     gene            1604..1954
                     /locus_tag="PK198_22845"
     CDS             1604..1954
                     /locus_tag="PK198_22845"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF41658.1"
                     /translation="MQNTERQVLIAQLRDALLALYGKRLSGIILYGSYAREDFNAESD
                     IDFLLILDEKPFDYAQEIRRITQITYPMMLEHDIIISCLPSPLERWRSEPSFFFDQVK
                     KDGIAIWTRNSNVS"
     gene            1927..2301
                     /locus_tag="PK198_22850"
     CDS             1927..2301
                     /locus_tag="PK198_22850"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRF41659.1"
                     /translation="MDEKLQRILNEAEQAISDAEFNLQGERCHVVANRTYYAVFHCIQ
                     ALLHSEGLFVKPHQGAQQKFHQLFIKTGKIPVEAGETLAYLAELRTLSDYDYGSTITT
                     QQAQKAIEMAAAFMETTRSFFT"
     gene            2314..2514
                     /locus_tag="PK198_22855"
     CDS             2314..2514
                     /locus_tag="PK198_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41660.1"
                     /translation="MSLLAQQLIAENKAQHARGEDASYLDLGTNAKLTELPDLSELEW
                     LNLETLEQRIAEVPQKLRDANV"
     gene            complement(2541..>3544)
                     /locus_tag="PK198_22860"
     CDS             complement(2541..>3544)
                     /locus_tag="PK198_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41661.1"
                     /translation="PDGQWNWAFREGKIADTRAVAITWTVENQVLIAGFFNKNTHIAG
                     QEFTANTSDRDAFLAAYAAETGLPRWAVKAGGVFDDDITALAADEQGRIYAVGYLVGV
                     MTLSPTLAIQSATGIPDFFILQYSGDGIPLRARALGGAQTNLATGIAVAGGRVLVGGH
                     YQGAMMWDGISLSNSAAFAGFVAAFDDQLQGLWAQRFPAAQGFFVNNMALAPDGRLWC
                     AGSFAGQADLPQGQFAAGGAFDGFVMSTADPVPVQQELAAPTWRVFPNPAADFIVIEN
                     FTPGERLRVFSASGLEMLGFERIGNVINIAVWPPGLYYLEVTGDETRSLLPFVKMRR"
     assembly_gap    3545..3585
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3586..4123)
                     /locus_tag="PK198_22865"
     CDS             complement(<3586..4123)
                     /locus_tag="PK198_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41662.1"
                     /translation="MWNLSPAAAQVWTDFFATSAPGIETIDAVALAPDGAIAVAGAYN
                     AAFSWRGAALPATEGQDVWLSCMNPDGEVRWAKSGGSILDDEVAAVVADADGNIIVAG
                     SFWLTGTFDGLTLHAGDNPKALFLLKYSPEGQLLWGRSIAGTGIKGLGGLATDPAGNV
                     YAAGFFGGALQVGDSTLQA"
BASE COUNT          977 a         1226 c         1226 g          890 t
ORIGIN      
        1 cagcaggcgg ttttcgggca tgaggtgcag ttcggagcgg gccgggagca gttggccgga
       61 aacggcatct cgcacggtag ccttcacata agtcaccgga gcggggcgca ggccgggcgg
      121 catttcaaat tgcaggatgt cggtattgat gcctgccgga ttgtcgccgg gtttggcatc
      181 ggggtcgccg gcatcggagg tgaaccaggc cgttgcgccg tccaagctga cgatgagcgc
      241 gccttcgttg gcggtggtgt tgatggggta gccgagattt tgcggtttcc cgaatatgcc
      301 ttccggcgat aaacggctga gaaacagatc gaaaccgccc atgccgggat gcccgtcgga
      361 catgaagtag agcgtttggc cgtcggggtg aaaaaacggg cagtcttcat tgccggcggt
      421 gttgatcgta gcgccgaggt tggccggcag gctccagtcg ccattgggtt tgcggcgcac
      481 caaccacaga tcgcggccgc cgtatccgcc gggccggtcg ctcgaaaaaa tgatgacctt
      541 gccgtcggcg gagagcgtgg gctggcgctc caggtacttg ctgtttgcgg ggtcgccgag
      601 gttgttgggg cggctccagc ggccatggat gaagtaagaa atgtagagat cgcaactgcc
      661 cagaccctcc ggcaggttgc aggcattgaa cactaaggtg cgcccgtcgg cggagatgct
      721 ctgtgcgccc tcgttgaagg gcgtgttgat atcggtcatg ggctgggctt tcgacgccgt
      781 cgggcggttt ccggctgagg taaaagtctt cctgccggtt gacgatgcgc gtaaacacca
      841 gcgtattgcc gtcggcggtg atcgagggca ggctttcggg catgggcgta ttgatactgt
      901 cgctcagtgg tttgggctga aagggcagcg gatttttcac ggcttcggcg gcaaagcggg
      961 cattggccaa atagcgttcg gcgcgttgtt tcaggttctc gtttcggccc ggcgattgca
     1021 aaaaccgctc aaaagccgtc gccgccgcat cgtatttttc caaacgcaac ctgctcattc
     1081 ccagcggata ccaggctttg gagtcgtaat cgggggccag tgcgagcgcc gcttcaaaca
     1141 gtttttccgc ctcggcatag tttttctcat cgtagcgaat ggcggctttc agcaattgcg
     1201 cgtcaatgaa ccggggcgct tcccgcaggg cggcctctgt ctctttgagg gcttcggcgt
     1261 tgcggcctgc ctggccggcg gctaatgccc gttcgtaaga tttgagtgct ttggccgggg
     1321 cgtctttgcg ggtcacaaaa tcctgcgcac ccagaaggct cggcaaagcg aaaaggcaac
     1381 tgaaaaggag tattttccac ataacaaaag cgtactgatg agttgatgga acaggccgtc
     1441 tctaaacgct gaaacgctcc gtgaattgca aaaaaaagct tccgggcagg tgagtccagc
     1501 cgcaggaagg tgaggggctt gctcccgcaa ctcaaaaact ttgcccccct tcaggacgtt
     1561 gcagatcata aaaactgcta tctttgaacc caaactgccg ctcatgcaaa atacagaacg
     1621 acaagtactt attgcacagc ttagagatgc cctgctggct ttatacggca agcgcctgtc
     1681 cggtattatt ttgtacggct cttatgcgcg ggaggatttc aacgccgaat cggacattga
     1741 ctttttgttg atcctcgatg aaaaaccatt cgattacgca caggaaatcc gccgcatcac
     1801 tcagatcacc taccccatga tgctcgaaca cgacatcatc atctcctgcc ttccctcccc
     1861 tttggaacgc tggcgctccg aacccagctt cttcttcgat caggttaaaa aagacggaat
     1921 tgcaatatgg acgagaaact ccaacgtatc ctgaacgagg cagaacaagc catcagcgat
     1981 gccgaattca atcttcaggg cgagcgctgc catgtagtcg ccaacagaac gtattatgct
     2041 gtatttcact gcattcaagc attgcttcac agtgaaggtt tgtttgtgaa accccatcag
     2101 ggcgcacagc aaaaatttca tcagcttttt atcaaaaccg gcaaaattcc cgtagaggcc
     2161 ggcgaaacgc ttgcttatct ggccgaactc cgaacactca gcgattacga ttatggctct
     2221 acgattacga cccaacaggc tcaaaaagcc atcgaaatgg ccgccgcttt tatggaaaca
     2281 acccgctcat ttttcacctg atttcgatat accatgagcc tcctcgctca acaactcatc
     2341 gccgaaaaca aagcccaaca cgcccggggc gaggatgcct cttaccttga cctcggcaca
     2401 aatgcgaaat taacggaact gccggacttg tcggagttgg agtggttaaa tttagaaacc
     2461 cttgaacagc ggatcgccga agtgccgcaa aagctgcgcg atgcgaatgt atagcaaacg
     2521 gtcggctgtt ttcagtggcg tcacctgcgc atcttgacaa aaggcaacaa actccgcgtt
     2581 tcgtctcctg tcacttccaa ataatacagc cccggcggcc agacggctat gttgatcaca
     2641 tttccaatcc tctcaaaacc aagcatttca aggcccgatg cagaaaagac gcgcagccgc
     2701 tcacccggtg taaaattttc tatcacaatg aaatcagcgg caggattggg aaaaacgcgc
     2761 catgttggag cggccaactc ctgctgcacc ggcaccggat cggcagttga cataacaaat
     2821 ccgtcgaaag cgccgcccgc cgcaaactgg ccttgcggca gatcagcctg ccccgcaaag
     2881 ctgcccgcgc accacaatcg cccgtcggga gcaagcgcca tattgttgac aaaaaaacct
     2941 tgcgcagccg gaaatcgctg tgcccacagg ccctgcaact gatcgtcgaa ggccgcgaca
     3001 aatcccgcga aagcggcgct gttgctcagc gaaatgccgt cccacatcat ggcgccctga
     3061 tagtgcccgc ccaccaacac gcgcccgccg gctaccgcaa tgccggtggc cagattggtc
     3121 tgagcgccgc ccaaagccct ggccctgagc ggaataccat caccgctgta ttgcaggata
     3181 aaaaaatcgg ggatgccggt agcgctctga atggcgaggg tgggcgaaag cgtcatcaca
     3241 cccaccagat acccgacggc gtaaatgcgg ccctgctcgt cggcagccag cgcggtgatg
     3301 tcgtcgtcaa acacgccgcc ggctttgaca gcccagcgcg gcagtccggt ttcggcggca
     3361 taagcggcga ggaaggcgtc gcggtcggag gtattggctg taaattcctg cccggcaatg
     3421 tgggtgtttt tattaaaaaa accggcaata agcacctgat tttcaaccgt ccaagtgatg
     3481 gccaccgcgc gggtgtcggc tattttgcct tcccggaaag cccagttcca ttgaccgtcg
     3541 ggagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgct tgcagggtgc
     3601 tgtctcccac ttggagcgcg ccgccgaaaa agcccgccgc atagacattg cctgccggat
     3661 cggtggccaa tccgcccagc cctttgatgc cggtacctgc tatggagcgg ccccagagga
     3721 gttggccttc cggcgaatac ttgagcaaaa aaagcgcttt gggattgtcg ccggcatgca
     3781 aagtcagccc gtcaaaggtg cccgtgagcc aaaaactgcc cgccacgatg atattgccgt
     3841 cggcatcggc cacaacggca gccacttcat catcgaggat gctgccgccc gatttggccc
     3901 aacgcacctc gccgtcggga ttcatgcaac tgagccacac atcctgaccc tctgttgccg
     3961 gcaaagcagc cccgcgccat gaaaaagcgg cattgtaggc gcctgctacg gcgatggccc
     4021 catcgggagc gagggctacg gcgtcaatgg tttcaatgcc gggagcggaa gtagcgaaaa
     4081 aatcagtcca gacctgagcg gcggcagggc tgagattcca caaaaaaatt attgcaaggc
     4141 aacagacaga gcggcggcga gcgatgtggg aacagttttg ccgggcgtcg gatttgagta
     4201 cctttgcgga aaattttcgc atgagccttt cttattcaaa cgtgttgttg gtggtattgt
     4261 tgagcctctc ggccttcgct tgcggcaaca aaaaagacgc cgcccccggc aaagctacat
     4321 cgggcaaccg gaccttggac tcgatcaacc tgctgattga
//
LOCUS       DAORLF010000415         4355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11011_length_4355_cov_2.394651, whole genome shotgun sequence.
ACCESSION   DAORLF010000415 DAORLF010000000
VERSION     DAORLF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11011_length_4355_cov_2.394651"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>233)
                     /locus_tag="PK198_22870"
     CDS             complement(<1..>233)
                     /locus_tag="PK198_22870"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41663.1"
                     /translation="LLIGWGMAGFEDLHWKGFDLYEARSAHFTIDLFFDRTTAVYFSV
                     TVILTFMVLMFSRFYIHREKGYKRFFNNILFFY"
     assembly_gap    234..285
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(455..3010)
                     /locus_tag="PK198_22875"
     CDS             complement(455..3010)
                     /locus_tag="PK198_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HRF41664.1"
                     /translation="MEKHSSRIFNEAHVLHQLEHYLPSQTPLKDFVHHNSLHAFQDQK
                     FYDAIFKASKMFGFQVTIQLNEFRKLYKINRIRREILESVIKERKGEQHLDAWLQKAL
                     QQQYDYNIEPRIGRLRALWKSHYHIDLDNLVQPLLFRILCSFLDQGVALWNFPFEEKG
                     LLGAIRTLEQNSFAGFIHGKRARKLLMDSSLELADLLKIVAGDEAFFEQYLFDQQFAH
                     RGWSGMVATVEHRPESLLYAKNISLRELIHLELIMEIDALDRALGTEKWQPLALVAQA
                     EPMDLFAEAAISEFHEVLMIWQDAFEWSYYDEVLAGLQHLKGFQSQTDEDIKDVSFQA
                     IFCIDEREDSLRRHIEMVDKRSETLGAPGFFGVEFFFQPSGGKFYEKLCPAPVTPKYL
                     IKEKETGPHHRKRELLYTKRSHTMLQGFVITLSLGLWAAVKLFLDLFRPKMSPAISDA
                     FAHMNMDAELQIESADPPTMENGLQLGFTVTEMADRVENLLRGIGMAERFARIVYMVG
                     HGSSSANNPHHGAHDCGACSGRPGAVNARVFAFMANHAKVRELLSARGMHIPAGTRFV
                     GALHDTAADEIAFYDADVLPQQLAKAHEQHQNAFEEALNLNAKERSRRFASIDTQADI
                     KKVREAIRRRSVSYFEPRPELGHGTNALCFVGHRGLTKGLFFDRRAFMNSYDYRTDPD
                     GRLLLNVIRPLPIVCGGINLEYYFSRVDNYKLGAGTKLPHNVMGLIGVANSSDGDLRP
                     GLPLQMIEVHDPVRLLILVEHYPDVVLKTIGAQDDLYDWFKDEWVHLVAIHPDTNDFY
                     YFKNEAFYLYKPVKESVERADDVHYLLESAKEMETNYIVDATQENLPVHILTE"
     gene            complement(3073..>4355)
                     /locus_tag="PK198_22880"
     CDS             complement(3073..>4355)
                     /locus_tag="PK198_22880"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF41665.1"
                     /translation="AGVIQILFGIFKLGKLADVFPLSAIHGMLAAIGIIIIAKQIPVL
                     LDIDPALTKGKEPLELLAEIPFFLSHIDPKATVIGVISLWVMTSWHNNTVPFLKKIPA
                     PLVVLLIAVPAQLIMDFQHTEPTYALVHIGNLLEVVKVNVSFDGFSQPGLFIKYVVMF
                     ALVGTLESLLTVKAIDMMDPYKRKSDTNKDLIAVGIGNTLAGILGGMPMISEVARSSN
                     NVANGAKTRWANFFHGFFILVFVLFAARYIEMIPNTALAAMLITVGIKLAHPREFAHI
                     FKIGKEQLAIFLVTIIITLYEDLLVGIAAGMALKIIIHVANGLPIRSLFKSPVSVSFE
                     DDNYLVEIEKSAVFTNWLGIKGKLDAIPQGMHLTIDLSNTKLVDHSVMENLHHFEHDY
                     VAAGGTVKIIGIDDHKALSKHDLAARKKRHTTSA"
BASE COUNT         1050 a         1102 c         1103 g         1048 t
ORIGIN      
        1 aataaaagaa caggatattg ttgaaaaatc gcttgtagcc cttttcccgg tgaatataaa
       61 agcgactgaa catcagtacc ataaaggtga gaataaccgt gacgctgaaa taaaccgccg
      121 tggtgcggtc aaaaaacagg tcaatggtaa aatgcgcgct tcgggcttcg tacaggtcga
      181 aacctttcca gtgcaggtcc tcgaaaccgg ccattcccca gccaatcaac aacnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtac cacgccggca
      301 gctattctga aaataggctt ctcctgttgg ttgctcggta tcaggctcaa caaaaagccg
      361 gccagcggca cgatgagaaa aaatggaagt aaatgatgca tacagcatgg ttttgtagta
      421 atcaaaaaga gtgcacggaa gaacgccgga tggcctattc ggtcaaaata tgcacgggca
      481 agttctcttg cgttgcatcc actatgtagt tggtttccat ctctttagca gattccaaca
      541 ggtaatgaac atcgtcggcg cgctccacgc tttctttgac cggcttgtac agatagaaag
      601 cctcgttttt gaagtagtaa aaatcgttcg tgtccgggtg gatggccacc aaatgcaccc
      661 attcgtcttt gaaccaatcg tacaaatcat cctgagcgcc aatggttttc agcactacgt
      721 cggggtaatg ctcgaccaga atcaacaggc ggacgggatc gtgtacctca atcatctgaa
      781 gcggcaggcc gggacgcagg tcgccgtcgc tgctgttggc cacgcctatc agtcccatga
      841 cgttgtgcgg cagtttggtg cccgcgccga gtttgtagtt gtctactctg gaaaaatagt
      901 actccagatt gatgcccccg caaacaatcg gcagcggccg gatgacattg agcaagagcc
      961 gtccatctgg gtcggtgcgg taatcgtagg agttcataaa agcgcggcgg tcaaaaaaca
     1021 gccctttggt aaggccgcga tgccccacaa aacaaagcgc gttggtacca tgccccagtt
     1081 cggggcgcgg ctcgaagtag gagaccgacc ggcggcgaat ggcttcccgc acctttttga
     1141 tgtcggcctg cgtatcaatg gaggcgaagc ggcgcgagcg ctctttggca ttcaggttga
     1201 gagcttcttc aaaagcattt tggtgttgct cgtgcgcttt cgccaactgc tgaggcaata
     1261 catcggcatc gtaaaaagca atttcatcgg ctgccgtgtc gtgcagggcg cccacaaacc
     1321 gggtgccggc aggaatgtgc attcctctgg cactgagtag ctcccgcact tttgcatggt
     1381 tggccatgaa ggcaaatacc cgggcgttga ccgcccccgg tcgcccgctg catgcgccgc
     1441 agtcgtgcgc gccgtgatgc gggttgttgg cgctgctcga accgtgcccc accatgtaaa
     1501 cgatgcgcgc aaaacgctct gccatcccaa tgccgcgcaa gaggttttcg acgcggtcgg
     1561 ccatttctgt cacggtgaag ccaagttgca ggccgttttc catggtcggc gggtcggcgc
     1621 tttctatctg caactccgcg tccatattca tgtgcgcgaa ggcatcggaa atagccgggc
     1681 tcattttggg ccggaacaaa tccaaaaaca gttttaccgc cgcccacaat cccagcgaca
     1741 gcgtaatgac aaagccctgc aacatggtgt gcgagcgctt ggtgtagagg agttcccgct
     1801 tgcgatgatg aggccccgtt tctttttctt tgatgaggta cttgggcgtg acgggcgccg
     1861 gacacagttt ttcgtaaaac ttgcccccgg aaggctggaa gaaaaattct acgccgaaaa
     1921 aaccaggcgc gcccagcgtt tcactgcgct tgtccaccat ttctatgtgg cgccgtaggg
     1981 agtcttcccg ctcgtctatg caaaaaatgg cctgaaacga aacgtcttta atgtcttcgt
     2041 ctgtctgcga ttgaaagccc ttgaggtgtt gcaggccggc cagtacttcg tcgtagtaac
     2101 tccactcaaa agcatcctgc cagatcatca gcacttcgtg aaactcggaa atggcagcct
     2161 ccgcaaacaa atccatcggt tcggcctgtg ccaccaaagc caagggttgc catttttctg
     2221 tccccagagc gcggtccagg gcatctattt ccataatcag ctccaagtgg atcagttcgc
     2281 gcaaggagat atttttggcg tataacaggc tttcggggcg gtgttctacg gtggccacca
     2341 tgcccgacca gccgcgatgt gcaaattgct gatcgaacag gtattgctcg aaaaaggctt
     2401 catcgcccgc tactattttc aagaggtcgg ccaattccag agatgaatcc atcagtagtt
     2461 tcctggcccg tttgccgtga atgaagccgg caaaactgtt ttgctccaaa gtgcggatgg
     2521 ctcccaacag gcctttttct tcaaatggaa aattccacag cgcaacgccc tgatccaaaa
     2581 agctgcacag gatgcgaaac aggagcggct gcaccaaatt gtctaaatca atgtggtagt
     2641 ggctcttcca caacgcccgc agtcgcccta tgcgcggctc tatgttgtag tcgtactgct
     2701 gctgaagcgc cttttgcagc caggcatcca agtgctgttc accttttcgc tctttgatga
     2761 cactttccaa aatttcccgc cggatgcggt tgattttata gagctttcgg aattcgttca
     2821 gttgtatggt cacctgaaat ccgaacattt tggatgcctt gaaaatggca tcataaaatt
     2881 tttgatcctg aaaagcgtgc agcgaattgt gatgcacgaa atctttcagc ggcgtttgcg
     2941 agggcaggta atgctccaat tggtgcaaaa cgtgtgcctc gttgaatata cgcgatgaat
     3001 gtttctccat cttgttggaa tgtattttaa ttgcgtaatc ggcctcgtag tcatgtacta
     3061 tccggcgcct tttcaggcgg aggtcgtatg tctttttttg cgtgccgcca gatcgtgttt
     3121 agagagcgct ttatggtcgt ctatgccgat gatcttcacc gtaccgcctg ctgccacata
     3181 atcgtgttca aagtggtgga ggttttccat cacagagtga tccaccaatt ttgtattcga
     3241 tagatcaatg gtgaggtgca tgccttgtgg aatggcatcc aacttgccct tgatacccaa
     3301 ccaattggtg aaaaccgcag atttttcaat ttctaccaaa tagttgtcgt cctcaaacga
     3361 aacactgacc ggcgatttga acaatgaccg gatcggaagg ccgttggcta catgaatgat
     3421 gatttttaat gccataccgg cagcaatgcc caccaacagg tcttcgtaca aagtgatgat
     3481 gatggtgacc aaaaaaatgg ccagttgctc cttcccgatt ttgaaaatat gcgcgaactc
     3541 tctgggatgc gccaatttga tgcccaccgt gatcagcatg gcggccaatg cggtattggg
     3601 aatcatctca atgtagcgcg ccgcgaacag cacaaacacc agtatgaaaa agccgtgaaa
     3661 gaaattggcc cagcgtgttt tagcgccgtt tgccacattg ttggaactac gcgccacctc
     3721 cgagatcata ggcattccgc ccaatatgcc ggccagggtg ttgccgatgc ccacagcaat
     3781 caaatcttta ttggtgtccg atttgcgctt gtaggggtcc atcatatcta tagccttcac
     3841 ggtcagcaag gattccagcg tgcccaccaa ggcaaacatg acgacgtatt tgataaaaag
     3901 ccccggttgt gaaaacccgt caaaactgac attcaccttc actacttcca gtaaattgcc
     3961 gatatgaact aaggcataag tcggctccgt gtgctgaaaa tccatgatga gctgcgctgg
     4021 aactgcgatg agcaatacca ctaagggcgc cggaattttt ttgagaaaag gcactgtatt
     4081 gttgtgccaa ctggtcatca cccacaagct gatgacgccg atgacggtgg ccttcgggtc
     4141 tatatgagat aagaaaaagg gaatttcggc cagcaattcc aatggttctt tgcctttggt
     4201 cagggccggg tcaatgtcta acagcaccgg tatttgcttg gcaatgatga tgatgccgat
     4261 ggccgccaac atgccgtgta tggctgaaag gggaaataca tcggccaatt tgcctaattt
     4321 gaaaatgcca aacaggattt gaatgacgcc ggcta
//
LOCUS       DAORLF010000416         4324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11113_length_4324_cov_2.006559, whole genome shotgun sequence.
ACCESSION   DAORLF010000416 DAORLF010000000
VERSION     DAORLF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11113_length_4324_cov_2.006559"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK198_22885"
     CDS             <1..181
                     /locus_tag="PK198_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41666.1"
                     /translation="LRQRIQGESQRAALLLSEIKKLRAEVSATRQRLDGINAALNEHQ
                     RANNAMLYGSFVALN"
     gene            360..695
                     /locus_tag="PK198_22890"
     CDS             360..695
                     /locus_tag="PK198_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41667.1"
                     /translation="MIDSTTDARTRQILEEFERLRAQLEPFTVNIHGGEGRLLRVSAT
                     LKAPANIMLQGQKIRLIDLALLAQNKLERFDASPSLRNTTQGEALEIVSQWIAKRAGF
                     FNYQTTKIV"
     gene            818..1519
                     /locus_tag="PK198_22895"
     CDS             818..1519
                     /locus_tag="PK198_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41668.1"
                     /translation="MKRQRARRAALYNEVAEAFKNVFGAVDFDKVAAFITERKDSERR
                     IEIVAAWLEHSGKLNGLPIRDLRKAVISDVFALPPEVQSLVVACAQLLPEDRAPEVLK
                     LYWKDSQFEAPEVTEAEKKRIQERHTVFATGAAAYRAGEIFHALIFAINRVNRRHPEH
                     RLPFHKIGEALPFMAPFVAQGPGEELVFNPDNCLFETGDGKPLKDEVLGLHVPAHLQH
                     LFTDEIDDNGPTAQP"
     gene            1986..2789
                     /locus_tag="PK198_22900"
     CDS             1986..2789
                     /locus_tag="PK198_22900"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF41669.1"
                     /translation="MLVDNTFTLITGASMGIGRCLALECASRGMNVLLVALEENLLAE
                     LAQNIRQQYGVEADFKPADLTQPADIRAVYDWCIERHYKVNMLLSNAGIGSGGIYENN
                     PLEKYRTLVLLNNMAAVQLTHHFLPMLKQNGPAWLMYTSSMEATIPIPFKAVYTASKH
                     FLYGFSLALKEELRDFGVSVSVLCPGPVATNEGSIARLKTQGKIGKLVVLTPEAVATH
                     AIKGLLAGRRVIVPGALPSLLEKLLLCIPRNLKMKLLKRVFGKYKEVQQ"
     gene            complement(2882..3427)
                     /locus_tag="PK198_22905"
     CDS             complement(2882..3427)
                     /locus_tag="PK198_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRF41670.1"
                     /translation="MKIVFAVAAPLLLAGMLFKAVPGNNKAASVAADEGAIQWMTWDE
                     AIQKSEKKPKKIFIDLYTDWCGWCKKMDASTFADPQVADYINKNFYAVKFNAEQRENI
                     VYKGYTMKFVENGRRGYHELAYSLLDGRLGYPSYVYLDEQQNRITISPGYKPVEPMMK
                     ELKYIAGGHYKTMTLDQYTPN"
     gene            3544..4107
                     /locus_tag="PK198_22910"
     CDS             3544..4107
                     /locus_tag="PK198_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRF41671.1"
                     /translation="MRKIVVAVGGSSGAIYAKVLLDKLLALQSQIEAVGVVMSDNARF
                     NWKFELDDESYLHYPFHFYNKNDFMAPFASGSARYDTMIVCPCSMGLMGRIAAGTSND
                     LATRAADVILKERRRLILVLRDTPYSLIHINNMKLITEAGGIICPATPSFYSKPATFE
                     ALAATVVDRVLDLAGFDLKSYRWGEEQ"
BASE COUNT         1082 a         1114 c         1107 g         1021 t
ORIGIN      
        1 cttgcgccaa cgcatccagg gcgaaagcca acgggccgcg ctgttgcttt ccgagatcaa
       61 gaaactacga gccgaggtaa gcgccaccag gcaacgcctg gacggcatta acgccgcgct
      121 caatgagcat caaagggcca acaatgccat gctttacggc agtttcgtag cgctgaattg
      181 acgcgccgcg catatatccg caaaaacgcg ggccttttgc gtttttttca tcaggcgaag
      241 ccgggcggac ggtcgaagcg ctgcacacag cgccccgaaa cgcgccccaa attgcattga
      301 aaaccaaagg cttaagcgat ttaaatgctt tttttcaaaa ccaataaaac tatatcaaaa
      361 tgattgattc aactaccgac gcccgaacgc gccaaattct tgaagaattt gagcgcttgc
      421 gcgcccagct tgagccgttt accgtcaaca ttcacggcgg tgaaggccgg cttttgcgcg
      481 tttcggcaac gctcaaggcg ccggccaaca tcatgctcca ggggcaaaaa atacgcctca
      541 ttgatctggc attacttgcc caaaataagt tggagcgctt cgatgcgtcg ccctcattgc
      601 gaaacactac ccagggcgaa gcgctcgaaa tcgtttcgca gtggattgca aaacgcgccg
      661 gatttttcaa ctaccaaacc accaaaatag tatgagcaaa ataaatcaaa tgcccgatta
      721 ttgggccgct ttcgaggcgc ccgacggcga aaaactcgca aaccgcaaca tcgagttttt
      781 ccaatatgaa agccgcatcc aggcagactt tgaaggcttg aagcgccaac gcgcccgacg
      841 tgccgccctt tataatgaag tggccgaagc cttcaaaaac gttttcggcg ccgttgactt
      901 tgacaaagtg gcggctttta ttacagagcg caaagactcg gagcgccgta ttgaaatagt
      961 tgccgcctgg ttggagcata gcggaaagct gaatggccta cccatcagag atttacggaa
     1021 agcggtaatt tctgacgtgt tcgccttgcc acctgaagtt caaagcctgg ttgtcgcttg
     1081 cgcccaatta ctgcctgaag atagagcgcc cgaagttctg aagttgtatt ggaaggattc
     1141 gcaatttgaa gcgcccgaag tgaccgaagc ggaaaaaaag cgcatccagg agcgacacac
     1201 ggtttttgcg accggggccg ccgcatatcg agccggtgaa atttttcacg cgctcatttt
     1261 tgctatcaat cgagtaaatc ggcgccaccc ggaacaccgg ctaccatttc ataaaatcgg
     1321 cgaagcgctg ccgtttatgg cgccctttgt ggcgcaaggc ccaggcgaag aactggtctt
     1381 taatcctgac aactgtttgt ttgagaccgg cgacgggaaa ccgttgaaag acgaggtttt
     1441 gggcttgcat gtgccggcac atttacaaca tcttttcact gatgaaatag acgataatgg
     1501 cccgaccgcg caaccctgaa acgataccgc gctctcaatg gtcaacggtt cgagttacca
     1561 gggagctaca ccaccgcgcc aaagtttttg ccgagcgccg acaaatgaaa ttgcaggacg
     1621 tgagcgccat tgccctaaaa acgtacctcg acgcgaaaag cccgcccacg ttcgcagatg
     1681 agatacgcgt ttatcttcgc gtcattagag aaaaaatgga ggcttgggat aggccaggcg
     1741 ccgaggctgt tttattggaa atcgagaaac gttttttgta ggtgttactt gcatgttttg
     1801 gcccgtcgga gaaatatccg gcgggctttt ttgtatccgg cggccaaaat ttggcttttc
     1861 gggctaaatt tgccgggaaa ggcaaaaaaa aatgtatttt cgtttgtttt ttgtaccttt
     1921 tcaatgtaag taactgacaa tcaaggcgca aaatatttgc ggccgaaatc aagaatgtaa
     1981 gcgagatgct tgttgacaat acatttaccc tcatcaccgg ggccagcatg ggcattggcc
     2041 gctgtctggc tttggaatgt gcctcgcggg gaatgaacgt gcttttagtg gcattggaag
     2101 aaaacctgct ggccgaactg gctcaaaaca tcagacaaca atatggcgtg gaggccgatt
     2161 tcaagcccgc cgatctgaca caacccgccg acatcagagc cgtgtacgac tggtgcatag
     2221 agcgtcatta caaggtcaac atgctgctca gcaatgccgg catcggcagt ggcggtattt
     2281 atgaaaacaa ccccttagag aagtaccgca cattggtact gctcaacaac atggccgccg
     2341 tacaactcac ccatcacttc ctgcccatgc tgaaacaaaa cgggccggcc tggctgatgt
     2401 acaccagcag catggaagcc accattccca ttccgttcaa ggcggtttat acggcgtcga
     2461 agcacttttt gtacggcttt tcgctggcgc ttaaagagga actgcgcgat ttcggcgtgt
     2521 cggtgagtgt attgtgcccc ggcccggtag ccaccaatga aggaagcatt gcccgactga
     2581 aaacacaggg taaaatagga aaattagttg tgctcacgcc cgaagcggtg gccacacacg
     2641 ccatcaaggg gctgctggcc gggcgccggg tgattgtgcc gggagccttg ccttcgctct
     2701 tggaaaaact cctgctttgc attccgagaa acctgaaaat gaagttattg aagcgagttt
     2761 tcggcaagta taaggaggtg cagcagtaac ttttttttcc aaaaaacaaa gccggccatg
     2821 acacatctca tcatgaccgg ctttgctgtt tttacattgt cggctcgtgg cccgcaagtt
     2881 cttaattggg cgtgtattga tccagcgtca ttgttttgta atgcccgccc gcaatatact
     2941 tcagttcttt catcatgggt tcaacgggtt tgtagcccgg cgagatggtg atgcggtttt
     3001 gctgctcatc gagatagaca tagcttggat agcccagtcg cccatcaagc agggagtacg
     3061 ccagttcgtg gtagccacgg cgtccgtttt ccacaaattt catcgtatag cctttataaa
     3121 cgatgttttc gcgctgttcg gcattgaatt tcacggcgta aaaattcttg ttgatgtaat
     3181 cggctacctg cggatcggca aaagtggaag cgtccatctt tttgcaccag ccgcaccagt
     3241 cggtgtacag gtcaatgaag attttttttg gttttttttc gcttttttgg atggcttcat
     3301 cccaggtcat ccattggatg gcgccttcgt cggcagcgac ggaagcggct ttgttgttgc
     3361 cgggaacggc cttaaaaagc ataccggcca acaggagcgg cgcggccaca gcgaaaacaa
     3421 ttttcatgtt catctatttt tagtttgatt caaaaacaag cgacgttcca aaacgccgaa
     3481 atgccggcct gcggacgaat cggcagggca gtttttgatt tttttaaaac aaccttaacg
     3541 tttatgcgca aaattgtggt agcagtagga ggttccagcg gagccattta tgccaaagta
     3601 ttgttggaca aactcttagc cttacaaagt caaatcgaag cggttggtgt ggtgatgagc
     3661 gacaatgcgc gcttcaactg gaagtttgag ctggatgacg agtcttacct ccactaccct
     3721 ttccattttt acaacaaaaa tgacttcatg gcgccctttg catccggctc ggcccgctac
     3781 gacaccatga tcgtgtgccc ctgctctatg ggactcatgg gccgcatcgc tgccggcacc
     3841 tccaacgatc tcgccacccg cgctgccgat gtaatattga aagagagaag gcgtttaatc
     3901 ctcgtgctgc gcgatacgcc atacagtctc atccacatca acaacatgaa gttgatcacc
     3961 gaagccgggg gaattatttg tccggctaca ccttcctttt acagcaaacc cgctaccttt
     4021 gaggccctcg ccgcgacggt ggtggaccgc gtgctggatt tggcaggctt cgacttgaaa
     4081 tcgtacagat ggggggagga acagtaggcg gtggccgcgc catggcgcgg ggtgggcggt
     4141 tccaacccgg aactttgaac ccgaaacccg aaaccttgaa cccgaaacct gcctgcgcca
     4201 aggcttcggc ggatgaaacc cggaaccttg aacgcggaac tcgaaacatt gaactcgaaa
     4261 tctttaatca accatacatt atgagaattt tgactttctt tgcggccctg atctgggtca
     4321 tcaa
//
LOCUS       DAORLF010000417         4292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11211_length_4292_cov_2.034222, whole genome shotgun sequence.
ACCESSION   DAORLF010000417 DAORLF010000000
VERSION     DAORLF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_4292_cov_2.034222"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>597)
                     /locus_tag="PK198_22915"
     CDS             complement(<1..>597)
                     /locus_tag="PK198_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF41672.1"
                     /translation="WHLALGTPAEKLRFKDHDNLAHYANAAVDIQFDFPFGFKELEGI
                     HSRTDFDLSSHQELSGKKMQYFDSELNENYVPYVVETSVGCDRMFLALMSQALQEESV
                     PDAKGEDATRTVLRLHPALAPVKCAVLPLLKNKEELTGKAREVFDSLKHSFLCQYDEK
                     DAIGRRYRRQDAIGTPFCVTIDFDTLQDDTVTIRERDS"
     assembly_gap    598..744
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<745..>971)
                     /locus_tag="PK198_22920"
     CDS             complement(<745..>971)
                     /locus_tag="PK198_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRF41673.1"
                     /translation="AELKAIIDDLEIADPDTGSRNWTEVRQFNLMFSTQLGNIAGEDG
                     KLYLRPETAQGIFVNFLNVHKTTRQKVPFGI"
     assembly_gap    972..1073
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1074..1478)
                     /locus_tag="PK198_22925"
     CDS             complement(<1074..1478)
                     /locus_tag="PK198_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRF41674.1"
                     /translation="MSQQADQFKKLVSHCKEYGFIFPSSEIYDGLSAVYDYGPYGAEL
                     KNNIKSYWWKAMVQMHENIVGLDAAIFMHPLTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DQLIEAEIDKLEAKIKKEVEKARGRFENFDEAM"
     gene            complement(1541..>1823)
                     /locus_tag="PK198_22930"
     CDS             complement(1541..>1823)
                     /locus_tag="PK198_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41675.1"
                     /translation="LDRDALWLSPEPGATVPPLSTLMRHAAFDTVLVFGYEPAQLGLQ
                     INWQHYAPIPFLGKTLLFSHSLPLIMEERNRNEKTYSGPLWAAVKHIFA"
     assembly_gap    1824..1833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2136..3224
                     /locus_tag="PK198_22935"
     CDS             2136..3224
                     /locus_tag="PK198_22935"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRF41676.1"
                     /translation="MKYTISLTKTENSKLGGVDFNNIPFGRHFSDHIFIADFDGENWV
                     NPRIEPFANFSIHPACMGLHYGQSIFEGMKATKTLDGQPMLFCPEMHAARMNASARRM
                     CMPEIPESLFLQGLDMLVGVDADWIPPQEGSALYLRPLMFANDEFIGVRPSAKYKFII
                     MTGPVGPYYPKPVRLMAENHFVRAVNGSTGEAKAAGNYAGAMLPTKMAQEKGFDQVMW
                     LESPEFKYIQEVGTMNIFFVINGVTITPVTDGAILKGITRDSIITLLREEGYPVEVRR
                     ITIDELVAAYKAGTLEEVFGAGTAAVVSHVSHIQYDELLMELPPVEGRKISRWVKDHI
                     DGLRSGRFEDTHGWVVPVGVFQEEEVPA"
     gene            3312..3695
                     /locus_tag="PK198_22940"
     CDS             3312..3695
                     /locus_tag="PK198_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41677.1"
                     /translation="MERIVSLLLIAALTLFNEAGSFPLQLPSVFHNPLDEEEAEKFFC
                     IEKESIEERSEEDHPDHWRHDSGGADDIAFVQQGLPAIGQPLPLHSFYDSVFQSITDR
                     AAHLLGTLVGRPFYILFHAFKAFLP"
     gene            3849..>4292
                     /locus_tag="PK198_22945"
     CDS             3849..>4292
                     /locus_tag="PK198_22945"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF41678.1"
                     /translation="MQQNTEKKTLPADGLAGLKAHGATDAISGFIVFLLALPLSLGIA
                     KASDFPPITGLLTAIIGGLVVSFLAGSRLTIKGPAAGLIVIVAGAVTDFGGGETGWKM
                     ALGAIVIAGVIQILFGVFKLGKLADVFPLSAIHGMLAAIGIIIIAK"
BASE COUNT          899 a         1041 c         1100 g          993 t
ORIGIN      
        1 tggagtcgcg ctcgcggatg gtgaccgtat cgtcttgcag cgtgtcgaag tcaatggtga
       61 cgcagaacgg cgtgccgatg gcgtcctgcc ggcggtagcg gcgaccaatg gcatcttttt
      121 cgtcgtattg gcaaaggaag gaatgcttga ggctgtcaaa tacctcgcgg gccttcccgg
      181 tgagttcttc tttgttttta agcagcggca gcacggcgca tttcacgggt gccagagccg
      241 gatgcaggcg cagcacggta cgcgtggcgt cttccccttt ggcgtcgggc acgctttctt
      301 cctgaagcgc ctggctcatc agggccaaaa acatgcggtc gcagccgacg gaggtttcca
      361 ccacatacgg cacatagttt tcgttgagtt cggagtcgaa atactgcatt tttttgcccg
      421 acaactcctg atggctgctg aggtcgaagt cggtgcggga gtgaatgcct tccaactctt
      481 tgaaaccgaa gggaaaatcg aactgaatgt ccacggcggc attggcgtag tgggccaggt
      541 tgtcgtggtc tttgaatcgc agtttttcag ccggcgtacc cagcgccaga tgccattnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnncgatgc cgaaggggac tttctggcgg gtggttttgt
      781 gtacgttgag gaaattgacg aagatgccct gcgctgtttc cgggcggaga tagagcttgc
      841 cgtcttcgcc ggcgatgttg cccaactgcg tggaaaacat gaggttgaac tggcgcactt
      901 cggtccagtt gcgcgagccg gtgtcggggt cggctatttc cagatcgtca atgatggcct
      961 tgagttcggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatggcc
     1081 tcgtcgaagt tctcgaaacg gccgcgcgct ttttccacct cctttttaat tttggcttcc
     1141 agtttgtcta tctcggcttc gatcaactgg tcggcgcggt agcgtttttt gctgtctttg
     1201 ttgtcaatga gcgggtcgtt gaaggcgtcc acatggccgg aagccttcca ggtgaggggg
     1261 tgcataaaga tggcggcgtc gaggcccacg atgttttcgt gcatttgcac cattgctttc
     1321 caccagtaag acttgatgtt gtttttcagt tcggcgccgt aaggtccgta atcatatact
     1381 gcgctgagac catcatatat ttcgctggaa ggaaaaataa acccgtattc tttgcaatgg
     1441 gaaactaatt ttttgaattg atcggcttgc tgcgacatga tttttgtgtt taatgcgatg
     1501 aaaaacaggc cgcaaagata gggtattcgg ggaattaacc tcacgcgaaa atatgtttca
     1561 ccgcagccca cagcggcccc gaataggttt tttcgttgcg gttgcgttct tccatgatga
     1621 gcggcagcga gtggctgaac aggagggttt tgcccaaaaa aggaatcggg gcgtagtgtt
     1681 gccaattgat ctgaagcccc agttgagccg gttcgtaccc gaataccagc accgtatcga
     1741 aggcggcatg gcgcatcaag gtggataaag gcggcacagt ggcgcccggt tccggcgaaa
     1801 gccagagcgc gtcccggtcg agtnnnnnnn nnncgatctg aacgcctctc agtattcttt
     1861 ccaaaaaagg cttcagcgcg gggtcttcca ccgcttccgt cactaccaga atgccgcgcg
     1921 cattgtctcc ctgccggttc aacatatttg catcggcatc gggaagatcg gttatttcaa
     1981 attccaacaa gacattcatc agaataaaga ttagcaatac aaagcattga caaattaaag
     2041 tctaaaaaac acccaaaatc agactgtttt taaaattatc atctgcattc ttggttataa
     2101 aggtaaaagt atgtactttt gcacaaaacc gcgtcatgaa atatacaatc agcctgacta
     2161 agaccgaaaa ttccaaactc ggcggagtgg atttcaacaa cattcctttc ggtcgtcatt
     2221 tctccgatca cattttcatc gctgatttcg acggcgaaaa ctgggtaaat ccgcgcattg
     2281 agccgtttgc caatttctcc atacacccgg cctgcatggg cctgcattac gggcagtcca
     2341 tatttgaggg catgaaagcc accaagacgc tcgacggcca acccatgctg ttctgcccgg
     2401 aaatgcacgc cgcccgtatg aacgcttcgg cacgccgtat gtgtatgccc gaaataccgg
     2461 aatcgctgtt tttgcaaggc ttggacatgc tcgtcggtgt ggatgccgac tggattccgc
     2521 cgcaggaagg cagcgccctg taccttcgcc cgctgatgtt tgccaatgac gagttcatcg
     2581 gcgtgcggcc ctctgccaaa tacaaattca tcatcatgac cggcccggta ggtccttact
     2641 accccaaacc agtgcgcctg atggccgaaa accactttgt gcgcgcagtg aacggcagca
     2701 cgggcgaggc caaggcggcc ggcaattacg cgggcgccat gcttcccacc aaaatggctc
     2761 aggaaaaagg tttcgatcag gtcatgtggc tcgaatcgcc ggagttcaag tacattcagg
     2821 aggtgggcac catgaatatt ttcttcgtca tcaacggagt aacgatcacg ccggtcaccg
     2881 acggggccat cctcaagggc atcacccgcg acagcatcat cacgctgctg cgcgaagagg
     2941 gctacccggt ggaggtacgc cgcatcacca tagacgagtt ggtagctgcc tacaaagccg
     3001 gcacattaga ggaggttttc ggcgcgggca cggctgccgt agtgtcgcat gtatctcata
     3061 ttcaatacga tgaactactg atggagctgc cgccggtaga aggccgcaaa atcagccgct
     3121 gggtgaaaga ccatattgac ggcttgcgct ccggtcgttt cgaggatacg catggctggg
     3181 tagtgcccgt gggcgtgttt caggaagagg aagtgcctgc ctgaattaac atcctgtttg
     3241 caaaaaatac ttggaatttc cgatgtagcc ctgctacctt tgccgccgat tacgatttca
     3301 attgaccggc agtggagaga atagtgtcat tattattgat tgctgcgctt acgttgttca
     3361 acgaagccgg ctcttttcca ttgcagcttc ccagtgtttt ccacaatcct ttggatgaag
     3421 aggaggccga aaaatttttc tgcatagaaa aagaaagcat tgaggagcga agcgaagaag
     3481 accatcccga tcattggcgc cacgattccg gcggcgccga cgatatagcg ttcgttcaac
     3541 agggcttgcc ggcaatagga cagcctttac ccctgcattc attctacgat tctgtttttc
     3601 aatctattac cgaccgtgcc gcccatctgc tcggcacctt ggtcggtcgt cccttttaca
     3661 ttctgttcca cgcgttcaag gcatttcttc cgtagtcgct aagaccggga agatgacgac
     3721 gggagggcga tggcttctcc cggcttccgt ccgcaggcgg tacgcttgtg cgctcgtcgc
     3781 gtgaaccatg taattgagta aaaacggcaa cacgtttcct ctgtattgcc ggaaagacag
     3841 attcaaccat gcaacaaaat acagaaaaaa agaccctccc ggcagacggc ctggcgggcc
     3901 tcaaagcgca tggcgccaca gacgccattt caggattcat tgttttttta ttggcgcttc
     3961 ccctgagttt gggcattgcc aaagcctcag attttccacc tattacagga cttctgacgg
     4021 ccatcatcgg cggcttggtc gtgagttttt tggccggatc gcggctgacc atcaaaggcc
     4081 cggcggcggg tttgatcgtc attgtagccg gggcggtcac agatttcggc gggggcgaga
     4141 caggttggaa aatggcatta ggcgctattg tgatagccgg cgtcattcaa atcctgtttg
     4201 gcgttttcaa attaggcaag ttagccgatg tatttcctct ttcagccata cacggcatgt
     4261 tggcggccat cggcatcatc atcattgcca ag
//
LOCUS       DAORLF010000418         4250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11336_length_4250_cov_2.124434, whole genome shotgun sequence.
ACCESSION   DAORLF010000418 DAORLF010000000
VERSION     DAORLF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11336_length_4250_cov_2.124434"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1244
                     /locus_tag="PK198_22950"
     CDS             114..1244
                     /locus_tag="PK198_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRF41679.1"
                     /translation="MQRIFFDNASTTPLDEHVIEAMTAAMRNLYGNPSSIHAEGRQAR
                     AAIEQARKTVAHRLGASIGEIFFTSGGTESSNMVLKNAVRDLGVRRIISSRIEHHCVL
                     HSVEALERQGLIRAEWVQLDAKGRVDMQHLETLLQSGAEKTLVSLMHSNNEIGAMIDL
                     PTVGALCREHGALFHSDTVQTIGYFDFRLADWPVDFITGSAHKFYGPKGTGFVYINGD
                     CMLKPFVDGGGQERNMRGGTENTAGIIGLATALELAYDHLQERRAHITGLRDYLRACI
                     EQAFPAARIYGDPDGGHYKLLNVGFPPTPRTDLLLLNLDIAGVSVSGGSACSSGADAG
                     SHVVEALGGEPGWKAVRFSFSHHNTREEVDVVMEKLKECLPL"
     gene            1389..1865
                     /locus_tag="PK198_22955"
     CDS             1389..1865
                     /locus_tag="PK198_22955"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRF41680.1"
                     /translation="MNHQDIQFLREAIRLAHEGPAQQAGGPFGAVVVKNGEIVGSSFN
                     RVPSTNDPTAHAEVMAIRDACARLGTFQLDGCILYASCEPCPMCLGAIYWARPDRVVY
                     AATQSDAAAIGFDDQFIYEQIELPPAQRSIPMTQLLRDEALGAFVAWTHIENKTEY"
     gene            1868..3529
                     /locus_tag="PK198_22960"
     CDS             1868..3529
                     /locus_tag="PK198_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF41681.1"
                     /translation="MPNRTPAFYTWAVALVAALFFLPFLGGVHLFDWDEINFAEAARE
                     MLALKDYLRVYINYEPFWEKPPFFFWLQALSMKAFGVGEYAARFPNAVCGILTVVLLF
                     RLGVRLRSVRFGLLWAGAYLGSTLPFLYFKSGIIDPWFNLFIFLGLYHFILHYWKKES
                     LPGIELARSAWWHLFWAGFFIGMGMLTKGPVALLIAGLTMAVYWVHQRFRLYVGVPQV
                     LFFLLASLIVGAAWLGLETWKNGPWFVREFFTYQYRLFSTPDAGHKGFPGYHLAVLAL
                     GCFPASVFAARSFFKMPAEPSAHLADLRRWMKFLFWVVVVLFSIVESKIVHYSSMAYF
                     PLTFLAASVIEKIWDGELPFARWMKVTLWSIAGLTAVLCIVVPILGMHPELLRDLIAD
                     PFGQANLDAQVHWSGWEVLPGLVVMAVLALFFFWKKRERTGRAFSVLFIRNAVFVLLA
                     LIFYIKRVEGYSQRAAVEFFQSKAEEDCYIITHGYKSYAHLFYAAKRPVTDPRSYDKD
                     LLLQGDAGKPVYVVCRIHRADELRAWPQLEEIGSKNGFVFFQRRP"
     gene            3563..>4250
                     /locus_tag="PK198_22965"
     CDS             3563..>4250
                     /locus_tag="PK198_22965"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41682.1"
                     /translation="MALQKLPIGIQDFRKIRENDFLYVDKTQYIHKMVSQSGAFFLSR
                     PRRFGKSITITTLQELYSGSRDLFKGLWIEDKWDWNKRHPVIRISFKDVNFEQLGLEK
                     ALFDRVEELSLRMEVPITRENARDQLREVIRSLGRERKVVVLIDEYDAPIIHYLGKDL
                     AQAQENRELLKGFYSILKELDSQLEFVFLTGVSKFSKVGIFSGLNNLQDISLHPAFAN
                     MLGYTQQELEG"
BASE COUNT          872 a         1179 c         1177 g         1022 t
ORIGIN      
        1 cggggtaggc ggtactcagc gcggcttgca gcttgtagcc agtagcttgt agccagcaac
       61 cgttccaacc cggaaccttg aacccgaaac tcgaaatcca aaaaatcctc ctcatgcaac
      121 gtatattttt cgacaacgca agtaccacgc cgctcgacga acacgtcatt gaagccatga
      181 ccgccgccat gcggaacctg tacggcaacc cctcctccat ccacgccgag ggcaggcagg
      241 cgcgcgccgc cattgagcag gcccgaaaaa ccgtggccca tcgcttgggt gcttccatcg
      301 gagagatttt tttcacttcc ggcggaacgg aatcgagcaa tatggtgctg aaaaatgccg
      361 tgcgcgatct cggtgtgcgg cgcatcatct cgtcccgcat agagcaccac tgcgttttgc
      421 actcagtgga agcgctggag cggcagggcc tgattcgcgc cgagtgggtg caattggatg
      481 ccaaaggccg cgtggacatg caacacttag aaaccttgct gcaaagcgga gcggaaaaaa
      541 ccctcgtgtc gctcatgcac tccaataacg aaatcggtgc catgatagac ctgcccactg
      601 tgggcgccct ctgccgcgaa cacggcgcgc ttttccactc cgataccgtg caaaccattg
      661 gttatttcga tttcaggctg gccgattggc cggtggattt cattaccggt tcggcgcata
      721 aattttacgg ccccaaaggc acaggctttg tttacatcaa cggcgactgc atgttgaaac
      781 cctttgtgga cggcggcggg caggaacgga atatgcgcgg cggcaccgaa aacacagccg
      841 gcatcattgg attggcaacg gcgctggaat tggcctacga ccacctgcag gagcgccggg
      901 cgcacataac cggtctgcgc gattacctcc gcgcctgcat tgaacaggca tttccggcag
      961 cccgcattta cggcgacccc gacggcgggc attacaaatt gctgaacgtg ggtttcccgc
     1021 ctacgccgcg caccgacctg ctgctgctca atttggacat tgccggcgtg agtgtatcgg
     1081 gcggcagcgc ttgtagcagc ggggccgatg caggttcgca tgtggtggag gcattgggcg
     1141 gcgaaccagg atggaaggcg gtgcgttttt cattttcaca ccacaatacc cgcgaagaag
     1201 tggacgtggt gatggagaag ctgaaggagt gtttgccgct ttagtaagtc ttcgcctttt
     1261 tgcagtttgc tacggactgc aacttatacc ttgcgccgcc aagttttgtg catagccttc
     1321 ggcataagtt attgattagc agcgcccggt atctcacctg accgtgcgag gcataattac
     1381 atgaatctat gaatcaccaa gacatccaat tcctccgcga agccattcgg ttggcgcacg
     1441 aaggccctgc gcaacaggcc ggcggaccat tcggcgcggt agtcgtcaaa aacggcgaaa
     1501 ttgtaggcag cagcttcaac cgcgtgccct ctaccaacga tcccactgcc catgcagaag
     1561 tgatggccat ccgcgacgcc tgcgcgcggc tcggcacttt tcaattggac ggctgcatcc
     1621 tgtatgcctc ctgcgagcct tgccccatgt gcctcggcgc catttattgg gcgcgccccg
     1681 accgcgtggt gtatgccgcc acacagtccg acgcagcagc catcggcttc gacgatcagt
     1741 ttatttacga acaaattgag ttgccgcctg cacagcgctc catccccatg acgcaattgc
     1801 tgcgagacga ggcactgggc gcgtttgtag cctggaccca tattgaaaat aaaaccgaat
     1861 attgatcgtg ccgaaccgta cgcctgcttt ttacacctgg gccgttgctc tcgtggctgc
     1921 gctttttttc ctgccctttc tgggcggcgt tcatttgttt gactgggacg agatcaattt
     1981 cgccgaggct gcccgcgaga tgctggcgct caaggactac ttgcgggtgt acatcaacta
     2041 cgagccattt tgggagaaac cgcccttctt cttttggctg caagccttgt ctatgaaggc
     2101 gttcggcgtg ggcgaatatg cggcccgatt tcccaatgcc gtgtgcggca tcctcacggt
     2161 ggtattgctg ttccggctcg gcgtgcgcct gcgctctgtg cgtttcgggc tgctgtgggc
     2221 cggagcatat ttgggttcca cgttgccgtt tttgtatttc aaatcgggca ttattgaccc
     2281 ctggttcaat ctgttcatct ttctgggctt gtaccatttc atcctgcatt actggaaaaa
     2341 agaatcgctt ccgggcattg aattggcccg ttcggcatgg tggcacctgt tttgggccgg
     2401 ctttttcatc ggcatgggca tgctcaccaa aggcccggta gccctgctca ttgccggcct
     2461 caccatggcg gtgtattggg tgcatcagcg tttccggttg tatgtgggcg tgccacaggt
     2521 attgtttttt ctgttggcct cgctcatcgt gggggcggcc tggttgggtt tggaaacctg
     2581 gaaaaacggc ccctggtttg tgcgcgaatt tttcacctac caatatcgtc tgttcagcac
     2641 ccccgacgcc ggccacaaag gctttcccgg ctaccatttg gccgttttgg ccttgggttg
     2701 ctttccggcg tcggtctttg ccgcgaggtc gtttttcaaa atgcccgccg agccttccgc
     2761 acaccttgcc gacctgcgcc gttggatgaa attcctcttt tgggtggtgg tcgttttgtt
     2821 ttccatcgtg gagtccaaaa ttgtgcatta ttcgtctatg gcctacttcc cgctcacgtt
     2881 tctggcggct tcggttatag aaaaaatatg ggacggcgag ctgccttttg cccgctggat
     2941 gaaggtgacg ttgtggagca ttgcaggttt gacggcggtt ctctgcattg tggtaccgat
     3001 attgggcatg caccccgaac tcctccgaga cctcatcgcc gatccttttg gccaagcaaa
     3061 tttggacgcg caggtgcatt ggagcggctg ggaagtgctg cctgggttgg tcgtaatggc
     3121 ggtgttggcc ttgtttttct tttggaaaaa aagagagcgc accgggcggg ccttcagtgt
     3181 gcttttcatc cgcaatgctg tgtttgtgct gttggcgctc attttttaca tcaaacgggt
     3241 ggagggctac tcgcaacggg cggcggtgga gtttttccaa agtaaagccg aagaagactg
     3301 ctacatcatc acacatggct acaaatcgta tgcgcacctg ttttacgccg ccaaacgccc
     3361 cgtcaccgac ccgcgcagct acgataaaga cctcctcctg caaggcgatg ccggcaaacc
     3421 tgtgtatgtg gtgtgcagaa tccaccgcgc cgacgaactc cgcgcctggc cccaacttga
     3481 ggaaatcggc agcaaaaatg ggttcgtatt ttttcaacgc aggccgtaga ttttcagtaa
     3541 ctttgtactc aaacggctac aaatggcact tcagaagcta cccatcggga tacaggattt
     3601 tcggaaaatc agagagaacg attttctgta tgtggacaag acgcagtaca ttcacaagat
     3661 ggtatctcag tccggtgctt tcttcctctc ccgcccgcgc cgcttcggca aatccataac
     3721 catcaccact ttgcaggagt tgtattccgg cagccgggac ttgttcaaag gcttgtggat
     3781 agaagataaa tgggactgga acaagcggca tccggtcatt cgcatttctt tcaaagatgt
     3841 caacttcgag cagttgggct tggaaaaagc cctgttcgac cgcgtggaag aactgagcct
     3901 gcgcatggag gttccgatca cccgcgaaaa tgccagagac cagcttcggg aggtcattcg
     3961 gtcgctgggt cgcgaacgaa aagtggttgt actcattgac gaatacgacg cgcccatcat
     4021 tcattatctg ggcaaagacc tggcgcaggc gcaggaaaac cgggagttgc tgaaaggctt
     4081 ttacagcatc ctgaaagaat tggactccca gttggaattc gtcttcctca ccggcgtgag
     4141 caagttttcc aaagtaggca ttttctccgg cctcaacaat ctgcaagaca tcagtctgca
     4201 cccggctttc gccaatatgc tgggctacac gcaacaggaa ttggagggaa
//
LOCUS       DAORLF010000419         4250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11337_length_4250_cov_2.019070, whole genome shotgun sequence.
ACCESSION   DAORLF010000419 DAORLF010000000
VERSION     DAORLF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11337_length_4250_cov_2.019070"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1225)
                     /locus_tag="PK198_22970"
     CDS             complement(23..1225)
                     /locus_tag="PK198_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41683.1"
                     /translation="MLRPILVFLLPVLFWNLAHAQKRLPIETGQKSAFFSSGLPEEEW
                     YQFGESNEVLDSLMAEIILLSGLTDVPQILVIQSNVENVSAVIFEGRRYLLWSLDFWE
                     QADPTMRLAALARELGHHLYEHSLLPELRAIEDREADRFMGYVLRRMNVPPVEMQEAL
                     SPSGFMKGQNYDRMAVILGGYKEADAQFRLDALPFDEDPAWLEFQKAAFPFPPPQCYQ
                     ALELPANYFPGHKTLGELSLRIKKALQEAQYPFRYLSVPDGFAVVTQIEQYEPDGKPL
                     KNAAQRWVELPVGATFSLSLDYLKSLVFPRKAYLRVFVFIVSKQAYASAEQRIGKEEA
                     KAWMQRGINRLPQSVQSRPSAGYNVDFLVYEFEVPEVNHKPGQKCPCLLDAGSHFRQS
                     GLERLFRP"
     gene            complement(1960..2277)
                     /locus_tag="PK198_22975"
     CDS             complement(1960..2277)
                     /locus_tag="PK198_22975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41684.1"
                     /translation="MPTARMQQLLHLLESAPGDSFLLFALAKEYEKAGDEEQALAHYE
                     QLRASDPGYVGLYYHLGKLLEKMDRSDDAKAAYDQGLETAKAAGDRHAWSELSAARMD
                     MDY"
     gene            2661..3401
                     /locus_tag="PK198_22980"
     CDS             2661..3401
                     /locus_tag="PK198_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRF41685.1"
                     /translation="MKLLVCVSKTPDTTSKIGFVNNGQEFDTAGIQYIMNPYDEWYAL
                     VRALELKEALGGSVTIINVGTADNDTVIRKGLAIGADDAVRIDADPKSAMFAAKQIAE
                     FAKNENYDIVFLGKETIDYNGAEIGSMIAEYLDMPFISYATSLNLNGTTASISRDIEG
                     GEEELEVDLPIVVSAAKGMAEQRIPNMRGIMMAKTKPLKVVPPVAFDDMAVTVSYTTP
                     PEKTSVKLVDPSNMEELVRLLHEEAKVI"
     gene            3435..>3900
                     /locus_tag="PK198_22985"
     CDS             3435..>3900
                     /locus_tag="PK198_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRF41686.1"
                     /translation="MVLVFAESQKGQFKKPAFEAVTYGYKTAQQMGVNCVALALGAAG
                     NPADLGKYGASKVLHVADASLDTLDSQVYASVIADVARQVAATVVVIPASTTGKSLLG
                     RLAVRLDAGSVAGVNTLPQTDGAFRVKRAVFSGKAIAEYEVKSGIKVLSLMGN"
     assembly_gap    3901..3948
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3949..>4250
                     /locus_tag="PK198_22990"
     CDS             <3949..>4250
                     /locus_tag="PK198_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF41687.1"
                     /translation="ESVSVAVPASKVRVKSVKRVEGIVPLPEAELVVSAGRGLKGPEN
                     WGMVEDLAAVLGATTACSRPVADAHWRPHHEHVGQTGIAIRPNLYIAIGISGAIQH"
BASE COUNT         1005 a         1114 c         1133 g          950 t
ORIGIN      
        1 ggaggagcat ggcaagttgg gtttatgggc gaaacaagcg ttctaggccg gattgccgga
       61 agtggctgcc ggcgtcgagc aggcaggggc atttttggcc gggcttgtga ttcacttcag
      121 gcacttcaaa ttcatacacc aaaaaatcta cgttgtagcc cgcagagggc ctgctttgta
      181 cgctttgggg caggcggttg atgccgcgtt gcatccaggc tttggcttct tccttgccga
      241 tgcgctgttc tgcggaagcg taagcctgtt tgctcacgat gaaaacaaac acccgcagat
      301 aggctttccg ggggaagacc aagcttttga gatagtccaa cgaaagtgaa aacgtagccc
      361 ctacgggcag ttcgacccat cgctgagccg catttttcag gggcttgccg tccggttcgt
      421 attgctctat ctgagtaacg accgcaaatc cgtcgggcac cgataaatag cgaaacggat
      481 actgtgcctc ctgaagcgct tttttgatgc gcagcgagag ctcgcccagc gttttatggc
      541 cggggaaata gttggccggc agttccagcg cctgataaca ctgcggcggc gggaagggga
      601 aagcggcttt ttggaattcg agccaggcgg ggtcttcatc gaagggcagg gcatcgaggc
      661 gaaactgtgc atctgcctct ttgtaccctc cgaggatgac ggccatgcgg tcgtagtttt
      721 ggcctttcat aaagccggat ggcgacagtg cctcctgcat ctcgacgggc ggcacgttca
      781 tgcgtcgcag tacgtacccc atgaagcggt cggcctcccg gtcttctatg gctcggagtt
      841 cggggaggag gctgtgttcg tacaggtggt ggcccagctc gcgcgccagg gccgccaggc
      901 gcatggtcgg gtcggcctgt tcccagaaat cgaggctcca gagcaggtag cgacgccctt
      961 caaatatgac ggccgataca ttctccacat tggactgaat cacaaggatt tgggggacat
     1021 ccgtcagtcc cgacaacagg ataatttcgg ccatcaggct gtccaatact tcgttgcttt
     1081 cgccgaattg gtaccactct tcctccggca gccccgacga gaaaaaagcg cttttttgcc
     1141 cggtttcaat gggcaggcgt ttctgggcat gggcaaggtt ccagaacaat accggcaata
     1201 ggaaaacaag gatcgggcgc agcataaggc gatgttttgc acaaacattc ataaaaaact
     1261 ctgaataaaa caagtttccg gcagtgcaca atttgacaaa tctcgaagat ttgtcaaatt
     1321 ttcgccccgc cccgctcgcg cgagattcca tctcgagcga gtatcctgcg ggttctaccc
     1381 gcttgtaaaa ccttcttttt cagcctgctc ccgcaaaagt agtgccgcct gtcgtgttga
     1441 cttcattcaa aaaaagaaaa cggcagcggg tagaacccgc acaatagtgg aacgagatag
     1501 aatctcgctc cagcagggcg aaccggttgc gaagcctcca aggattcgta accgtaaacc
     1561 cggcagtacg tttacgaaac ttttggaagt ttcgcaaacg cgcacgattc aataatccat
     1621 atccatcctt gccgccgctc ctctccggaa tgccggcagc gtgatcgaac tgtccagccg
     1681 tttgtcctgc atccctttgg ggatcaaata ctcgcacgct tcgctcccgt ggtacgccgc
     1741 ccggtggaag cgggagcgat tttttattca aaaaaaggga atggcagcgg gtagaacccg
     1801 cacaatagtg gaacgagatg gaatctcgct ccagcgggac accagcgctt cgcggtcttt
     1861 gcaatcctcg cgggataaat agcccgcaaa gagcgctaag ttcgcaaagc tctcctcgga
     1921 atgccaaacg aagagggcct agtgtaggct ccgcacgact caataatcca tatccatcct
     1981 tgccgccgac aactcgctcc aggcgtggcg gtcaccggcg gctttggcgg tttccaaacc
     2041 ctgatcgtag gcagctttgg cgtcgtcgga acgatccatt ttttccaaca acttgccaag
     2101 gtggtagtac aagcccacat agccggggtc gcttgcgcgc aactgctcgt aatgcgccag
     2161 agcctgctcc tcatcgccgg ctttttcgta ttctttggcg agggcaaaca gcaggaagga
     2221 atccccggga gcgctttcca aaagatggag caattgttgc attcttgcgg taggcatggt
     2281 agttgttttt attttacaaa aatgagcatt attaaaaagc tgcaagccat aagctacaag
     2341 ccataagctg tgacttttcc cttgcgagct tgattttcga taatgaaaaa agcattcttc
     2401 attcaatggg cattttgaaa tttcccccaa tagaaaatct aatttcacaa cgaaaaaacc
     2461 agtaactcga agctcgcggc tcgaagctgt tgaaagtcct gaataattcg agactccata
     2521 attattagcg aaaattcgct gcgaaccatt ttcttccggg cgaatttata tctttgtgcc
     2581 gcgaaaatca aatcgcgctg cgacactttt accaaccaaa aacaggcgga aacgcataat
     2641 gccgcatcaa ccaacaactt atgaaactat tagtctgtgt cagtaaaacg cccgatacta
     2701 cctccaagat cggttttgta aacaacggcc aggaattcga caccgccggc attcagtaca
     2761 ttatgaatcc ctacgacgag tggtacgctc tggtgcgcgc cctcgaactc aaggaagcgc
     2821 tgggcggttc ggtgaccatc atcaacgtcg gtactgccga caacgatacc gtcatccgca
     2881 agggattggc catcggcgcc gacgatgccg tgcgcattga tgccgacccg aaaagcgcca
     2941 tgtttgcggc caaacaaatt gccgaatttg ccaaaaacga gaactacgat atcgttttcc
     3001 tcggcaagga aaccattgac tacaacggcg ctgaaatagg ctccatgatt gccgagtacc
     3061 tcgatatgcc tttcatttct tacgccactt cgctcaacct gaacggcact acggcctcca
     3121 tttcgcgcga tattgaaggc ggagaagaag aattggaggt ggatttgccc atcgtggtga
     3181 gcgccgccaa aggtatggcc gaacaacgca ttcccaacat gcgcggcatt atgatggcca
     3241 aaaccaagcc gctcaaagtg gtgccgcctg tagctttcga cgacatggca gtgacggtgt
     3301 cttacactac gccgccggaa aaaacttccg tcaaattggt ggacccgtcc aacatggagg
     3361 aattggtgcg cctgctgcac gaagaagcta aagtaatcta acatatttgc aaacagatta
     3421 aaccagataa tatcatggta cttgtttttg ctgaaagtca aaaaggacaa ttcaaaaaac
     3481 cggctttcga ggccgtcacc tacggatata aaaccgccca acaaatgggc gtcaactgcg
     3541 tagcccttgc attgggcgct gccggcaacc cggccgatct gggcaaatac ggcgcatcca
     3601 aagtgctgca tgttgccgat gcttcattgg acaccttaga cagtcaggtg tatgcctccg
     3661 tcattgcgga tgtggcccgg caggtggccg ctacggtggt ggtgattccg gccagcacga
     3721 ccggaaaatc gctgctcggt cgccttgcgg tgcgtttgga tgcaggctcg gtggcgggtg
     3781 tcaatacgct gccccaaacc gatggagctt tccgggtgaa acgcgccgtg ttttcaggca
     3841 aagccattgc ggaatacgaa gtcaaatccg gcatcaaagt gttgtcgctg atgggcaatg
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag agtctgtttc
     3961 ggtagccgtt cccgcttcga aagtgcgcgt gaagtcggtg aagcgggtgg aaggcattgt
     4021 gccgctgccc gaagcggaat tggtggtgtc ggccggtcgc ggtttgaaag gccctgaaaa
     4081 ctggggtatg gtggaagatt tggctgctgt tttgggcgcc actacggctt gttcccgtcc
     4141 ggtagccgat gcacattggc ggcctcacca cgagcatgtg ggccagacag gtattgccat
     4201 tcggcccaac ttgtacatcg ccatcggtat ttcgggcgcc attcagcact
//
LOCUS       DAORLF010000420         4245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11355_length_4245_cov_2.067542, whole genome shotgun sequence.
ACCESSION   DAORLF010000420 DAORLF010000000
VERSION     DAORLF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11355_length_4245_cov_2.067542"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..235
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            238..>635
                     /locus_tag="PK198_22995"
     CDS             238..>635
                     /locus_tag="PK198_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41688.1"
                     /translation="MKFLLPGCLLLFAGWLSAQNGIVRGNVFDKESGEPIIYGTVRLL
                     NTSLGANTDVEGFFAIPNVPPGSYQLVATYVGYDSVVVDVSVKAGGIVFKSIYLNAAA
                     IELGGVEISARKEQARADVQISKITVTTKQ"
     assembly_gap    636..661
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <662..>1322
                     /locus_tag="PK198_23000"
     CDS             <662..>1322
                     /locus_tag="PK198_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HRF41689.1"
                     /translation="EPDIAQYLPVLPGIISSGDQGGQLYIRGGAPVQNRILLDGMTIY
                     NPFHSIGFFSVFETEAIRSVDVLTGGFNAEHGGRISAIVDIKTREGNRKNFGGIVSAS
                     PFQGKVLLEGPIKKLKDTGGGSTSFLLTGKHSYLDQTSKVLYKYATDTSFYSFAAGDT
                     SLADVLKDVGLPFSFTDLYGKLSFIGDNGSKLHLFGFNFTDRFSLPNIADLEWTNTGG
                     GA"
     assembly_gap    1323..1438
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1439..>1842
                     /locus_tag="PK198_23005"
     CDS             <1439..>1842
                     /locus_tag="PK198_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41690.1"
                     /translation="RTSAINTFTVNMNFTSFHDKGEVNYGFDFTGFNTDFAFRNALNI
                     GFAQKDFNTEIAGFIKLKQQLGNIVFEPGLRMQYFASQSDMQIEPRLGVKINISEGLR
                     FKFAGGLYSQNLISTVNELDVVNLFVGFLAGP"
     assembly_gap    1843..1907
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1908..2593
                     /locus_tag="PK198_23010"
     CDS             <1908..2593
                     /locus_tag="PK198_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF41691.1"
                     /translation="AFHAIAGLEIDLGKNTELNVEPYYKGFTQLISINRNKLSSADPD
                     FETETGRAYGIDFSLRHEVKDLYLWATYSLSYVDRDDGRQQYFTVFDRRHNANFLATY
                     NFGKKRLWEAGLRWNLGSGFPFTRTQGFYQNVNFQDLLLTDVLTGNFPLGTILSEKRN
                     DGRLSYYHRMDISLKRRFELGGTSKIDATLSVTNAYNRENIFFVDRITNNRVNQLPIL
                     PSLALTYSF"
     gene            complement(2664..>3415)
                     /locus_tag="PK198_23015"
     CDS             complement(2664..>3415)
                     /locus_tag="PK198_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF41692.1"
                     /translation="DGISQGLLKNTFLMASIASAALGIFSFFLPATPPKVSKGEKLSV
                     SEMLGLDALKLLKDRNYAVFFVSSILICIPLAFYYQNANPFLSEIGMSNPTGKMTIGQ
                     ASEVIFMLLLPLFFKRYGIKKTLLVGMLAWVLRYALFAFGDAGSNAYMLLLGIALHGI
                     CYDFFFVSGQIYTDSKAGEKFKSAAQGLITLATYGVGMLIGFWVAGLVSDAYAVDGGH
                     AWKNIWLIPSAIAGVILLLFAVAFRDNSKTA"
     assembly_gap    3416..3461
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3462..>3848)
                     /locus_tag="PK198_23020"
     CDS             complement(<3462..>3848)
                     /locus_tag="PK198_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF41693.1"
                     /translation="LEFFIWGAWFVTLGTFLGANLGASGAQTAAAFSTQSFGAIIAPF
                     IIGLIADKYFNAERILGVLHLCGAGLMYLMYGSSDFGAFYPYVFAYMVLYMPTLALVN
                     SVSFNQMKDPAKEFSVIRVWGTVGWIA"
     assembly_gap    3849..3867
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3993..>4245)
                     /locus_tag="PK198_23025"
     CDS             complement(3993..>4245)
                     /locus_tag="PK198_23025"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF41694.1"
                     /translation="KVSLGVMPDYVYDGEGMRVDGVTDGKPAQAAGVQKGDIIIGIGD
                     VQVKTIQDYMGGLSKFNTGDATVLKINRNGQVLELSVKF"
BASE COUNT          972 a         1090 c          992 g          898 t
ORIGIN      
        1 cgcgaaaatt cgcgcccttc gcagaaattc gcgttaaaaa attcgcgttt cctcgcgttt
       61 agcacctcgc gccatagcgc gcccaccgcc accgcctacc gcccactgtt tcccctcctg
      121 caagccaccc gacaaagcgc cccttgtttt gacgctgaat aatgctacct ttgcgcggct
      181 tttgcataca ggcaaagcct cactcgacaa aatgnnnnnn nnnnnnnnnn nnnnnaactg
      241 aaatttttac ttccgggttg cctcctgctt ttcgccggct ggctgagcgc ccaaaacggc
      301 attgtacgcg gcaatgtatt cgacaaagaa agcggagaac ccatcattta cggcaccgtg
      361 cgcctgctca ataccagcct cggcgccaat accgatgtag aaggcttttt tgccatccct
      421 aatgtaccgc ccggcagcta ccaattagtg gctacttatg tggggtatga cagcgtcgtg
      481 gtggacgtca gcgtgaaagc cggcggtatt gtttttaaaa gcatttacct caacgcagcc
      541 gctatcgaat tgggcggggt cgaaatatct gcccgcaagg agcaggcccg cgccgatgtg
      601 cagatttcca aaatcaccgt cactaccaaa caaatnnnnn nnnnnnnnnn nnnnnnnnnn
      661 ncgaaccaga tattgcacaa tatctgcccg tgctgccggg gatcatttcc tccggcgacc
      721 aaggtggcca attgtacatc cggggcggtg cgcccgtgca aaaccgcatc ctgttggacg
      781 gtatgacgat ttacaacccc ttccactcca tcggcttttt ctcggtgttt gaaacagagg
      841 ccatccgcag tgtggacgta ctcaccggcg gtttcaatgc cgagcatggc gggcgcatat
      901 cggccatcgt ggacatcaaa acccgcgagg gcaaccgcaa aaatttcggc ggcatcgtct
      961 cggccagccc gtttcagggt aaagtattgc tggaaggtcc catcaaaaaa ctcaaagaca
     1021 ccggcggcgg cagcacctcg tttttgctca caggcaagca ttcctatctg gatcaaacct
     1081 ccaaagtgct gtacaaatac gccacagata ccagtttcta ctccttcgcc gccggcgaca
     1141 ccagtctggc agacgtatta aaagacgtag gactgccctt cagcttcacc gatttgtacg
     1201 gaaaactttc tttcatcggc gacaacggca gcaaattgca cctcttcggc ttcaatttta
     1261 cagaccgttt cagcctgccc aacatagccg atctggaatg gaccaatacc ggcggcggcg
     1321 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     1441 gcaccagcgc catcaacact tttacggtga acatgaattt tacctccttc catgacaaag
     1501 gagaggtcaa ttatgggttt gattttacgg gtttcaacac cgactttgct ttccgcaatg
     1561 cgctcaatat cggttttgca caaaaagatt tcaatactga aatcgcaggt tttatcaaac
     1621 tcaaacaaca gcttggcaac atcgtcttcg agccgggcct gagaatgcag tatttcgctt
     1681 cccaatcgga catgcagatc gaaccacgcc tcggcgtcaa gatcaatatc agcgaggggc
     1741 tgcggttcaa atttgccggc ggtctgtatt cgcaaaacct catcagtacg gtcaacgagt
     1801 tggatgtggt caacctgttc gtcgggttcc tcgccggccc cgnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg ccttccatgc
     1921 cattgcgggc ctggaaattg atcttggcaa aaacacagaa ctcaatgtgg agccttacta
     1981 caaaggcttt acacaactca tcagcatcaa ccggaacaag ctgtcttctg ccgatcccga
     2041 ttttgaaacc gaaacaggcc gggcttacgg cattgatttt tcgctgcgcc atgaggtgaa
     2101 agacctctac ctgtgggcta cttactccct ttcgtatgtg gatcgcgatg acgggcggca
     2161 acagtatttc acggtgttcg accgccgcca caatgccaat ttcctagcca cctacaactt
     2221 cggcaaaaag cgcctttggg aagccggcct ccgctggaat cttggatcgg gattcccttt
     2281 cacgcgcaca cagggttttt accaaaatgt caatttccag gatttgctgc tcaccgatgt
     2341 gctgacgggc aactttccgc tgggtaccat cctgtctgaa aagcgcaacg acggacgcct
     2401 ttcttattac cacagaatgg acatttcgct caagcgccgc tttgaactgg gcggcaccag
     2461 caaaatagat gctaccctga gcgtgacaaa tgcctacaac cgcgagaata tcttcttcgt
     2521 ggaccgcatc accaacaacc gagtcaatca gttgccgatt ctgccgagtt tggcgctgac
     2581 gtattcgttt tgatgtgagg ggtacaaaaa aagcccgttt ctcaatgttg ggaaacgggc
     2641 tttttttatt ggagcgtatt ttctcaggca gtcttgctgt tgtccctgaa agctaccgca
     2701 aacagcagca aaatcacccc ggcgatggcc gaaggaatga gccagatgtt tttccaggca
     2761 tggccgccgt ccaccgcata agcgtctgat accaagccgg ctacccagaa tccgatgagc
     2821 atgcccacgc cgtaagtggc caacgtgatg aggccctgcg ccgcgctttt gaatttctcg
     2881 ccggctttgg aatcggtata aatctggccg gaaacgaaga agaaatcata gcaaataccg
     2941 tgcagcgcaa tgcccaaaag gagcatgtac gcattgctgc ccgcatcgcc gaacgcaaag
     3001 agcgcgtagc gaagtaccca ggccaacatg cccaccagca gcgtcttctt gatgccgtag
     3061 cgcttgaaaa acaaaggcag aagcagcata aaaatgactt ccgacgcctg cccgatggtc
     3121 attttgccgg tgggattgct catgccgatt tcagacaaaa acgggttggc attttgataa
     3181 taaaaggcca gcggaatgca gatcaggatg gaggaaacaa agaagacggc gtagttgcgg
     3241 tctttcagca gtttcagggc atccaagccc agcatctcgc tcacgctcag tttttcgcct
     3301 ttggacactt tgggaggcgt ggccggcagg aagaaactga aaatgcccaa tgccgccgat
     3361 gcgatggagg ccatgaggaa ggtgtttttc aaaagtccct gtgaaatgcc gtcctnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcaatccag cccacagtac
     3481 cccaaacgcg aatgacggaa aactccttgg cggggtcttt catctgattg aacgacacag
     3541 agttgaccaa tgccagcgtg ggcatgtaaa gtaccatgta agcaaaaacg tagggataaa
     3601 acgccccgaa atcgctgctg ccatacatga ggtacatcag tccggcgccg cagaggtgca
     3661 gcacgccgag gatgcgttcg gcgttgaaat atttgtcggc aatgaggccg atgatgaagg
     3721 gggcgatgat ggcgccgaag gattgggtgg aaaaagcggc tgcggtttgc gcgccggaag
     3781 cgccgaggtt ggctcccaaa aaggtgccga gggtaacgaa ccaggctccc caaataaaaa
     3841 attcgaggnn nnnnnnnnnn nnnnnnnagt tgcaggtagg tggttgtttt catgaataca
     3901 gattaaagtt ggttgtacgg cagatggctg cgccgtttgt ttggcgcttg cagggcaaag
     3961 gtatcatttt tgatgaatta ttggattgcc ccttaaaact taaccgacaa ctccagcacc
     4021 tgcccgtttc gattgatttt caaaacagta gcatctcctg tattgaactt ggacaggccg
     4081 cccatgtaat cctgtatggt tttgacctgc acatcgccta tgccgatgat gatatcgcct
     4141 ttctgtacgc cggcagcctg agccggtttg ccgtcggtga cgccgtccac gcgcataccc
     4201 tcgccgtcat acacatagtc gggcatcacg ccgaggctca ctttg
//
LOCUS       DAORLF010000421         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11413_length_4228_cov_2.156003, whole genome shotgun sequence.
ACCESSION   DAORLF010000421 DAORLF010000000
VERSION     DAORLF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11413_length_4228_cov_2.156003"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>380
                     /locus_tag="PK198_23030"
     CDS             <1..>380
                     /locus_tag="PK198_23030"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764426.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRF41695.1"
                     /translation="QFLSGIEFSVFALTDGHSWALLPEAKDYKRIGEGDTGLNTGGMG
                     AVSPVPFVDAELMDKVKTRIIEPTVRGIAARGMAYHGFVFFGLIRVDGEPYVIEYNCR
                     LGDPETEVVLPRLQNDLVELFVAM"
     assembly_gap    381..489
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <490..712
                     /locus_tag="PK198_23035"
     CDS             <490..712
                     /locus_tag="PK198_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRF41696.1"
                     /translation="KPIGGLEWVEGSMVFHAGMRAQEDGQLLTDGGRVMAVTSYGADF
                     REALALSNVNAGRIFFEGKYHRRDIGLDL"
     assembly_gap    1536..1545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1546..>1889
                     /locus_tag="PK198_23040"
     CDS             <1546..>1889
                     /locus_tag="PK198_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HRF41697.1"
                     /translation="PDDMHRRIAAELARIEQRYANPVSEEEMYELLAQFRYIIPQGSP
                     MAGIGNDFQASSLSNCFVIGNNADSYGGIIRTDEEQVQLMKRRGGVGHDLSHIRPKGS
                     PVMNSALSSTGIV"
     assembly_gap    1890..1899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1900..3973
                     /locus_tag="PK198_23045"
     CDS             <1900..3973
                     /locus_tag="PK198_23045"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRF41698.1"
                     /translation="SALTSTGLVPFMERFSNSTREVAQDGRRGALMLTISIQHPDAED
                     FIDAKLEAGKVTGANVSVRMDDAFMRAAVSGQTYRQQYPVDADTPAMVKDIDAQKLWN
                     KIIFNAWKSAEPGVLFWDTIIRESVPDCYADLGYRTISTNPCGEIPLCPYDSCRLLAI
                     NLFSYVDEPFTPQASFNFDKFRRHAQFALRMMDDIIDMEIEKVEHILEKIKNDPEADD
                     IKHTEQVLWEKVLHACINGRRTGVGITAEGDMLAALGLRYGSDEAIEFAVRVQRELAV
                     NAYRSSVQLARERGAFPIYDAAREMRNPFIQRLREADPALYDDMIKYGRRNIALLTIA
                     PTGTTSLMSQTTSGIEPVFMVSYLRRRKVNPNDKDVRIDFVDEVGDHWEEYHVFHHKF
                     ADWIRAQGHDMAAVARMSKDEIQPLIEQSPYHRATANDVDWVQKVKMQGAIQKWVDHS
                     ISVTVNVPEDTSVDMVKQIYQTAWESGCKGCTIYRDGSRSGVLVSESKKTKPEAATPA
                     GFPPKRPKKLEADIVRFTNNNERWMAFIGLVDGRPYEIFTGRAEDIFHLPEYVTKGWV
                     IKAADDEGRKRYDFQFTDREGYRVTIEGLSRSFNKEYWNYAKLISGVLRQGMPLNKVV
                     ELVGGLNVEDDHINTWKNGVVRALKRFVPDGTKVEKDPCPHCHSDTGLTYKEGCVICT
                     DCGYSECG"
     gene            4015..>4228
                     /locus_tag="PK198_23050"
     CDS             4015..>4228
                     /locus_tag="PK198_23050"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF41699.1"
                     /translation="MADDNTSVHNPHDRFFKSLMHNRETAIAFLEEFLPDEVKEVLDL
                     QSLELANTTFLTDELKQYFSDIVFKLK"
BASE COUNT         1005 a         1049 c         1111 g          934 t
ORIGIN      
        1 agcagttttt gagcggcata gaattttctg tgtttgcgct caccgacggc cattcctggg
       61 cgctgctgcc cgaagccaaa gactacaagc gcatcggcga gggcgatacc gggctgaata
      121 cgggcggtat gggcgctgta tctcccgtgc cgtttgtgga cgccgaactg atggacaagg
      181 taaaaacccg catcatagaa cccactgtgc gcggcatagc ggcgcggggc atggcttacc
      241 acggttttgt ctttttcggg ctgattcggg tggacggaga gccttatgtg atagagtaca
      301 actgtcgcct cggcgacccc gaaaccgagg tggtgctgcc gcgcctgcaa aatgatttgg
      361 tggaactctt tgtggctatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnc aagcccatcg gcgggttgga atgggtggag ggcagcatgg tttttcatgc
      541 gggcatgagg gcacaggaag acggccaact gttgaccgac ggcggccggg tgatggctgt
      601 gacctcctac ggtgcagatt tccgggaagc gctggccctg tcgaatgtca atgctgggcg
      661 tatttttttt gagggcaagt accatcggcg cgacatcggt ctggatttgt gagaatgtgg
      721 tagcagtgca ttttggagcg gtatttgggc ttcaatggcc gtgttttttg aaaaaagtgc
      781 attttttttc aaaaaaaaat tcaaaaacgc ataaaatgaa gttttgtatt tttcacaata
      841 cctcaattaa aagttattat aattaaaggt ttttttgact tttaagtgcg gtgtattttt
      901 ttcacaactt ggtaattatg tatttgtttc gggcgtgtca ggggctgcat atttgtatcg
      961 tgaagagaga gaagcagggt tgacacaggg aaatcaaaag gattttccgc acgaagcaag
     1021 gatatgttca tgggattata attttacacg ccatgaagtc ccggattgaa ttttcaaccc
     1081 gggactcatg ggcgacttcc cgcctcgctt cctccgaaat tgaatatgtt catgggatta
     1141 taatatcgta cggtcgcatc tcacaagatg cgaccttttt tcctcccctg attcaatttc
     1201 gacctgattt gcgttcatcc ataagatata tacttggttt agttggtatc acaatattgt
     1261 caagggtata gcgcctgatc ccgcgctata cccttcgtct ttttaggaaa aatcattttt
     1321 ttatcgcacc tcaaagccct atcttcaccc cgtcaaacac aaactactat ggagaccaaa
     1381 atgaagagcg cccaacgcgc cctacccaca aaaatttatg ccgccaaaga agccatcgaa
     1441 gcctcggttc aatatttcaa cggagacgaa ctggccgcca atgtatgggt aaacaaatac
     1501 gccctcaagg attccttcgg caatatttac gaacannnnn nnnnncccga cgatatgcac
     1561 cgccgcatag ccgccgaact ggcccgcata gagcagcgct acgccaatcc ggtatcggaa
     1621 gaagagatgt acgaacttct ggcgcagttt cgctacatca tcccgcaggg cagccccatg
     1681 gccggcatcg gcaatgattt tcaggcatct tcgctgtcca attgttttgt catcggcaac
     1741 aacgccgact cctacggcgg catcatccgc actgatgaag agcaggtaca actgatgaag
     1801 cggcgcggcg gcgtgggcca cgacctcagt cacatccgac ccaaaggcag cccggtgatg
     1861 aacagtgccc tcagttctac cggtattgtn nnnnnnnnnc tcggccctga cttccaccgg
     1921 gctcgtaccc ttcatggagc gcttttccaa ttctacccgc gaggtggcgc aggacggtcg
     1981 ccggggcgca ctcatgctca ccatatccat acaacaccct gatgcggagg attttataga
     2041 tgccaaattg gaggccggca aagtgaccgg cgccaacgta agcgtgcgca tggacgatgc
     2101 cttcatgcgg gcggcggtga gcggccaaac ctatcggcag caatatccgg tagatgccga
     2161 cacacccgcc atggtgaagg atatagatgc gcagaaactc tggaacaaga tcattttcaa
     2221 cgcctggaaa agcgccgaac cgggggtgct gttttgggat accatcatcc gcgagtcggt
     2281 gcccgattgt tatgccgatc tgggctaccg caccatttcc accaatccct gcggcgagat
     2341 tccgctttgc ccttacgaca gttgccgcct gctcgccatc aacctcttta gctatgtgga
     2401 tgagccgttt acgccacagg caagttttaa tttcgataaa ttccgccgcc atgcacagtt
     2461 cgccctccgc atgatggacg atatcattga tatggaaatt gagaaagtgg agcacatttt
     2521 ggaaaaaatc aaaaacgacc ccgaagcaga cgatatcaag cacacggagc aggtgttgtg
     2581 ggaaaaagta ttgcacgcct gcatcaacgg gcggcgcacg ggggtgggta tcactgccga
     2641 aggcgatatg ctggcagcct taggcttgcg ttacggcagc gacgaggcga ttgaatttgc
     2701 cgttcgggtg cagcgggagt tggctgtcaa tgcctaccgc tcgtccgtac aattggcccg
     2761 cgagcgcggc gctttcccca tttacgatgc cgcccgcgaa atgcgcaatc cgttcatcca
     2821 gcgtctgcgc gaagccgatc ctgccctgta cgacgatatg atcaaatatg gccgccgcaa
     2881 catcgcgctg ctcaccatag cgcccaccgg caccaccagc ctgatgagcc agaccacgtc
     2941 gggcatagag cctgtgttca tggtgtcgta tttgcgccgt cggaaggtga accccaacga
     3001 caaagacgtg cgcattgact ttgtggacga ggtgggcgac cactgggagg agtaccatgt
     3061 gtttcaccac aagtttgccg actggattcg ggcgcaaggc cacgacatgg cggctgtggc
     3121 cagaatgagc aaggatgaaa tacagcccct catcgaacaa tcgccgtacc accgggccac
     3181 cgccaacgat gtggactggg tacaaaaggt gaaaatgcag ggtgccatcc aaaaatgggt
     3241 ggaccactcc atcagcgtga cggtgaatgt accggaagac acctcggtgg atatggtgaa
     3301 gcagatttac caaacggcct gggaatcagg ctgcaagggc tgtaccatct accgagacgg
     3361 ctcccgttcg ggcgtgctgg tatcggagag caaaaagacg aagcctgaag ctgccacgcc
     3421 ggccggattt ccgcccaagc ggcccaaaaa actcgaagcc gacattgtgc gcttcaccaa
     3481 caacaacgag cgctggatgg cattcatcgg cttggtggac gggcgaccgt atgagatttt
     3541 caccggccgc gccgaagata tttttcatct gccggaatac gttaccaaag gttgggtcat
     3601 caaagctgcc gatgacgagg gcagaaagcg ctatgatttt cagtttaccg accgagaggg
     3661 ctaccgcgtc accattgaag ggctgagccg ctcgtttaat aaagagtact ggaactatgc
     3721 caagctcatt tccggcgtgc tgcgccaggg tatgccgctg aataaagtag tggagcttgt
     3781 gggcggcctc aatgtggaag acgatcacat caatacttgg aaaaacgggg tggtgcgcgc
     3841 cctcaagcgc tttgttcccg acggcaccaa agtggaaaag gacccttgcc cgcactgcca
     3901 ttccgatacc ggtctgactt ataaagaggg ctgtgtgatt tgcacagatt gcggttattc
     3961 ggagtgtggg taggaaggaa agtataaaat gaagtaaaaa aatcagattg catcatggcc
     4021 gacgacaata ccagcgtcca caatccgcac gaccgcttct tcaagtctct gatgcacaat
     4081 cgggagacgg ccattgcttt tttagaggaa tttctgcccg atgaagtgaa ggaggtactg
     4141 gatttgcaaa gtctggaatt ggccaatacc acttttctga ccgacgaact gaagcagtat
     4201 ttttccgaca ttgttttcaa actgaaaa
//
LOCUS       DAORLF010000422         4213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11468_length_4213_cov_1.507937, whole genome shotgun sequence.
ACCESSION   DAORLF010000422 DAORLF010000000
VERSION     DAORLF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11468_length_4213_cov_1.507937"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1210
                     /locus_tag="PK198_23055"
     CDS             <1..>1210
                     /locus_tag="PK198_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF41700.1"
                     /translation="ISGDLKAAYCLTEPGSGSDALAAKTRADLSTDGSHYVLNGQKMW
                     ISNAGFADIFIVFAQVDGDKFTGFIVERDTPGFSLGAEEDKMGIKGSSTRQVFFENCP
                     VPAENVLGEIGKGHLIAFNALNIGRFKLCSLSLGGATMCNTRSIEYAKERVQFKQPIA
                     NFGAIQYKLAEQAIRTFAGESALYRVSDLLEDMKNHFLEQGDDYAQAQLKAAEEYAME
                     CAILKVVGSEVLDYVVDENVQVHGGMGYSEEGTAARAYRDARINRIYEGTNEINRLLL
                     VDMLFRRALKGAIDIVGPAWEVQKELASMPSMEKVEGPYGEEERALRDFKKIILMTAG
                     AAAKMQMDGKLNLKEEQEIVMNVADMMIDTFVAESLLLRVQKLSGMSSLPVSQEVYDA
                     MLRTVFHDTTA"
     assembly_gap    1211..1231
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1522..2040)
                     /locus_tag="PK198_23060"
     CDS             complement(1522..2040)
                     /locus_tag="PK198_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRF41701.1"
                     /translation="MTELAGFRMGPFALMDLIGHDVNYTVTETVFQSFFYDPRYRPSF
                     TQKRLVEAGRLGRKSGRGFYIYKDGAAEPDSRYETATPYTEELGRQIVRRIVVMLINE
                     AADALFWGIASREDIDLAMTLGVNYPKGLLRWADEWGAAECVQILDGLYDEYREDRYR
                     CSPLLRRVGDGK"
     gene            complement(2037..2672)
                     /locus_tag="PK198_23065"
     CDS             complement(2037..2672)
                     /locus_tag="PK198_23065"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRF41702.1"
                     /translation="MTIGIIGAGSMGSGIAQVAATAGHRVVLYDAAAEAVAKALAGME
                     KTLGQLETKGKLPAGGAAAILSRIEPAQELSGLQAAECVIEAIVENLEIKKSLFRALE
                     PLVSPHCILATNTSSLSVTALAAVCERPERCIGLHFFNPAPIMRLVELAPALQTSEAV
                     MEQAETLMRQWGKTPVRVKDTPGFIVNRVARPFYGEALRILDEGMAGITGP"
     assembly_gap    2912..3107
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3375..>4213
                     /locus_tag="PK198_23070"
     CDS             3375..>4213
                     /locus_tag="PK198_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRF41703.1"
                     /translation="MTAIKFGTDGWRAIIAQEYTVDNVKRVAEGTARWMLQQGMKQVA
                     IGHDTRFAGQLFAETSARVFGALGIKVVIAKGYVSTPMVSLATARTGSGLGVVITASH
                     NPPSYNGFKLKSHLGGPLAPAQIGEVEDLIPDHCALELPELDELQSQGLLEYLNMEQM
                     YLDHVYANFDLDAIRKANIPMAFDAMYGAGQNVMRTLFPDAHLLHCEYNPSFMGQAPE
                     PIHRNLGELSQLIANSPDIALGLANDGDADRIGMYDQDGRFVDSHHILLLLLLYLYRY
                     KGER"
BASE COUNT          888 a         1150 c         1118 g          840 t
ORIGIN      
        1 catctcgggc gacctgaagg ctgcttactg cctcaccgag ccgggcagcg gctcggatgc
       61 attggcggcc aaaacccgcg ccgaccttag caccgacggg tcacattacg tcctgaacgg
      121 ccaaaaaatg tggatttcca acgcgggctt tgcagatatt ttcatcgtgt ttgctcaggt
      181 ggacggggac aagttcaccg gcttcatcgt ggagcgcgat acgccgggtt tctcgctggg
      241 cgccgaggaa gacaaaatgg gcatcaaggg ttcttccacc cgtcaggtgt tttttgaaaa
      301 ctgccccgtg cccgccgaaa acgtgctggg cgaaatcggc aaaggccacc tcattgcgtt
      361 caacgcgctc aatatcggtc gtttcaagct gtgctcgctg agtctgggcg gcgctacaat
      421 gtgcaacacc cgctccatcg agtatgccaa agagcgcgtg cagttcaaac agccgatcgc
      481 caacttcggg gccatacagt acaaattagc cgaacaggcc atccgcacct tcgccggcga
      541 aagcgcgctg tatcgcgtgt cggacttgct ggaagatatg aaaaatcact tcttggagca
      601 gggcgacgac tacgcgcagg cacagctcaa agcggctgag gaatacgcta tggaatgcgc
      661 catcctgaag gtagtaggtt cggaagtttt ggactatgtg gtggacgaaa acgtgcaggt
      721 acacggcggc atgggctact cggaggaggg cacggcagcc cgcgcttacc gcgacgcccg
      781 catcaaccgg atctacgagg gcaccaacga gatcaaccgc ctgctgctgg tggacatgct
      841 gttccgccgg gcgctcaaag gcgcgattga tatcgtagga ccggcctggg aagtgcaaaa
      901 agaattggcc tccatgcctt cgatggaaaa agtggaaggc ccttacggcg aagaagaacg
      961 cgcactgcgg gatttcaaaa aaatcatcct gatgacggcg ggcgctgcgg ccaaaatgca
     1021 gatggacggc aaactgaatc tgaaagaaga gcaggaaatc gtgatgaatg tggccgatat
     1081 gatgattgac acctttgtgg cggaatcact gctgctgcgg gtgcagaaat tgtcgggcat
     1141 gtcttcgctg ccggtatcac aggaggtgta cgacgccatg ttgcgcaccg tattccacga
     1201 taccacggca nnnnnnnnnn nnnnnnnnnn naaaatgaat aaaaacgcta cggatgccat
     1261 ctcttccttt gcggagggcg atctgttgcg cactttcctc atgggcctca aacgctatac
     1321 gaagtatccg ccggtgaacg tcagaaatac gcgccgcttg gtggcgtcga agttgattga
     1381 agcgggaggg tattgttttt aggggggagt aggtgagggg tgaggggtga gtgtgcgagg
     1441 acaccaaggc tccgccggat ggatagagag gtgaggaggt tggcttcagg gccggcctcc
     1501 tcattttttt tggtgggaat ctcacttccc atcccccacc cttctcagca gcgggctgca
     1561 tctatagcgg tcttccctgt attcgtcgta caggccgtcc aaaatttgca cgcattcagc
     1621 cgcgccccac tcgtcggccc agcgcagcaa acccttgggg tagttgacgc ccagggtcat
     1681 ggccaaatct atgtcctcgc gggaagcgat gccccaaaac agcgcatcgg cggcttcgtt
     1741 gatgagcatg accacgatgc ggcgaacgat ctgccggccc agttcttcgg tataaggcgt
     1801 ggcggtttcg taacggctgt caggctcggc ggcgccatcc ttatatatgt agaacccgcg
     1861 gcccgacttg cgccccagcc gcccggcctc taccaagcgc ttttgtgtga acgaggggcg
     1921 atagcgcggg tcgtaaaaaa acgactggaa caccgtttcc gtcaccgtgt aatttacgtc
     1981 gtggccgatg aggtccatca gcgcaaacgg ccccatgcga aagcccgcca gttcggtcat
     2041 ggcccggtta tgcccgccat gccttcgtcc aaaatgcgca gcgcctcgcc gtaaaatggc
     2101 cgcgccacgc ggttgacgat gaagcccggc gtatctttca cccgcaccgg cgttttgccc
     2161 cattggcgca tcagggtttc ggcctgttcc atcaccgcct cgcttgtttg caatgcaggc
     2221 gcaagctcca ccaagcgcat gatgggcgcc ggattgaaaa aatgcagccc gatgcacctt
     2281 tccgggcgct cacaaacggc tgccagggcc gtcaccgaca aagaagaggt gttggtagcc
     2341 aatatgcaat gcggcgatac caatggctcc aatgctctga acaggctttt tttgatctct
     2401 aagttttcca cgatggcctc aatgacgcac tccgccgcct gcaatcccga caattcctgc
     2461 gccggttcta tccgactcag gatggcggcg gcccctccgg cggggagttt acccttggtt
     2521 tccaattggc ccagtgtctt ttccataccg gccagcgctt ttgcaacagc ctcagcagca
     2581 gcatcgtaca acacaacccg atgcccggcc gtagccgcta cttgcgcaat gccgctgccc
     2641 atagaaccgg cgccgatgat gcctattgtc ataatgagtg attttgagga gcaaaggtaa
     2701 gtgccggggg cgggaatgta aaacgggaaa aaatacatga aattttcttc atattaaacc
     2761 gcgcagccac tgccggcgtt ttgctgtttt ttcattcata aaaaaatcag accatgataa
     2821 agagcatact gcccgtactg acggcttttc tgttttttac tttcgctcaa tcctgcgaat
     2881 ctcaggccca atctgccaaa ggtggcggcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct acctgatcta
     3121 ctgccgcagc ggcaatcgca gcggacaggc ctgcggcatc atgcagcaac agggattcgg
     3181 caaactctat aatttacagg gcggcatgat cgcctggggg cagcagtaag tccttctggg
     3241 cggcaagtca ggcggtcgct ttggggtgac ggcctgactt cgcgctgcca agacgcaaaa
     3301 ccccacagtt tttttaccac aaacatgccc cggaaccccg cacaagccgt acttttgccg
     3361 caaatccatt tgccatgaca gcaattaaat tcggaacaga cggctggcgg gccatcatcg
     3421 cacaagaata taccgttgac aatgtgaagc gcgtagccga gggcactgca cgctggatgc
     3481 tccaacaggg catgaagcaa gtggctattg gccatgacac gcgttttgcc gggcagcttt
     3541 ttgccgaaac ctccgctcgc gttttcggcg cattgggtat caaagtagta atagccaagg
     3601 gctacgtctc cactccgatg gtgtctttgg caacagccag aacaggctcc ggcctcggcg
     3661 tggtcatcac ggccagtcac aacccgcctt cctacaacgg gttcaaactc aaatcgcatc
     3721 tcggcggccc gctcgcgccc gctcaaatcg gcgaggtgga agacctgatt cccgatcatt
     3781 gcgcattaga actgcccgaa ttggacgaac tccaaagtca gggtctgctc gaatatctga
     3841 acatggagca gatgtacctc gaccatgtgt atgccaattt cgaccttgac gccattcgca
     3901 aggccaatat tcccatggcc ttcgatgcca tgtacggcgc cggccagaac gtgatgcgca
     3961 cgctcttccc cgatgcgcac ctgctgcatt gcgagtacaa tccttccttc atggggcagg
     4021 cgccggaacc cattcaccgc aacctcggcg agctttccca actcatcgcc aactcgccgg
     4081 acatcgcgct gggcttggcc aatgacggcg atgcagaccg catcggtatg tacgatcagg
     4141 acggccgctt cgtggattct caccatattt tgctgctgct gttgctgtat ttgtatcgct
     4201 acaaaggcga gcg
//
LOCUS       DAORLF010000423         4210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11482_length_4210_cov_2.048616, whole genome shotgun sequence.
ACCESSION   DAORLF010000423 DAORLF010000000
VERSION     DAORLF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11482_length_4210_cov_2.048616"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK198_23075"
     CDS             <1..328
                     /locus_tag="PK198_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41704.1"
                     /translation="PEYFVRNGYQPEPGGPAYSYEPVSVEGLFEHRTEKEENVSFGLY
                     NAAGEPVQMFFENKSWWAGQHRFKFTFSVWGFPGGEHEARRLVSLDALGMINSGRVLG
                     SKRVSW"
     gene            complement(541..2028)
                     /locus_tag="PK198_23080"
     CDS             complement(541..2028)
                     /locus_tag="PK198_23080"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41705.1"
                     /translation="MSLTDSTATIAWPAVAGAAYYKYAVSQGTTCHDDAPVLSSSENT
                     VSLSGLLPLTEYTFCLRAHCSCTSTGSTTVRFTTPARPLPNDFCATALAASCGSTITG
                     NTEGAFPDISQGICEGSNLLPGHGVWYQIQGDGSIMTLDLCASSFDTKVHVYEGACGS
                     LRCVASNDDACGAQSSLSFPSLPNQNYYILVSGADDAFGEFTMDINCVCQLPLESPWT
                     TASIGAGQGSAVENLCNGSIDLNYEGNAPVNQADRQIFTSQNACDTFSLITRVHFIHP
                     EAFAGIAIRENDAPGARMIALKSKGTKYVYLDTRTATDGARNTQQAITQNHRWLRLTR
                     AGDVFTAYTSADGLVWQQHFVVNMSMPDCLQAGLFVENAQNGANSTGVFTGTSIVPAA
                     GGTSLPGGDIPTGDFPMEFTVSPNPALTELRVAMSSVFSGKGLSISIVNQLGQTMTTR
                     KIEMVENLIETFDVQAFPPGVYVVCLRSEGSPLVVKKCVVGGRKY"
BASE COUNT         1070 a         1069 c         1128 g          943 t
ORIGIN      
        1 gcctgaatac tttgtgcgca acggctatca accggaaccg ggcggcccgg cctactctta
       61 cgaacctgta tcggtggagg gcttgtttga gcatcgcacc gaaaaagaag agaacgtgag
      121 ttttggccta tacaacgctg cgggcgaacc ggtacaaatg ttttttgaaa acaaatcctg
      181 gtgggccggc cagcaccgtt tcaagtttac attcagcgtg tggggcttcc ccggcggcga
      241 acacgaagcg cgccgtctcg tcagtctcga tgccctcggg atgatcaaca gcggcagggt
      301 actgggcagc aagcgggtga gttggtagaa aagagaaggg gagaccattc atccggcctc
      361 ccgctggcgc gagattccat ctcgtgccac tatccggtgg attcatatcc gccaaatact
      421 cgccttcggt agtttttgca agcgggtgga acccgcataa tactcacgcg agatagaatc
      481 tcggcgcgag cggaagagaa ggggagacca ttcattcggc ctccccctcc cgtaggcggg
      541 tcaatatttc cggcctccga caacacactt tttcacgacg agagggctgc cctcggaacg
      601 aaggcaaacc acatacacgc ccggcggaaa tgcctgtacg tcaaaggttt ctatgagatt
      661 ttccaccatt tctatttttc gcgtcgtcat ggtttgcccc aattgattca cgatgctgat
      721 gctgagaccc ttgcccgaaa aaacagaact catggctacg cgcaactcgg taagtgccgg
      781 gttgggagat acggtaaatt ccatcggaaa atcaccggtg ggaatatcgc cgccgggcag
      841 cgatgtacct cctgccgccg ggacaatgct cgtgccggta aagactccgg tggagttggc
      901 gccattttgg gcattctcga caaaaaggcc ggcctgaagg caatcgggca tagacatatt
      961 caccacaaaa tgctgttgcc acacaaggcc gtcggctgag gtgtaggcgg taaaaacatc
     1021 gccggcccgc gtcagtcgca gccaccgatg gttctgtgtg atggcttgtt gcgtattcct
     1081 ggcgccgtcg gttgcggtgc gcgtatcgag atagacgtac ttggtgcctt tggatttcag
     1141 ggcaatcatt ctggcgcccg gcgcgtcatt ttcccgaatg gcaatgcctg caaaggcctc
     1201 cgggtgtatg aaatggacgc gggtaatgag gctgaaagta tcgcaggcat tttgggatgt
     1261 aaatatttgc cggtctgcct gattcacggg agcgttgccc tcgtagttga ggtctatact
     1321 gccgttgcag agattttcga cagcgctgcc ctgccccgct ccgatggagg ccgttgtcca
     1381 gggcgattcc aacggcagtt ggcacacaca attgatgtcc atcgtaaact cgccgaaggc
     1441 atcgtctgcg ccgctcacca aaatgtagta gttctggttg ggcaggctcg gaaagctcag
     1501 gctggactgc gcgccgcagg catcgtcatt gcttgccacg catcggagcg aaccgcaggc
     1561 gccctcatac acatgcactt tggtatcgaa agaagaggcg cacaggtcta aggtcattat
     1621 agaaccatcg ccttgtatct gataccagac gccatggccg ggcaggagat tgctcccttc
     1681 gcagataccc tgactgatgt cgggaaatgc gccctccgta ttgccggtga tggtactgcc
     1741 gcaacttgct gccagagcgg tggcgcaaaa atcgttgggc aaggggcggg cgggcgtggt
     1801 gaaacggacg gtggtgctgc ccgtgctggt acaggagcaa tgagcgcgca ggcagaatgt
     1861 atattcggtg agcggcaaca aacccgacag ggagacagtg ttttccgaac tgctcagcac
     1921 aggcgcatcg tcatggcaag tcgttccctg cgacaccgca tatttgtaat acgcagcccc
     1981 tgcaacggcc ggccatgcga tggtagcggt ggagtcggtc aggctcacca atgacgccgc
     2041 tgcgggcgca gaacactggg agccgccagc caatgtgaga gtaaatacac tgctctgata
     2101 accataaaca tagatgtaat aattttcccc cgcactggaa tgccagctcg ttaaagcccc
     2161 atacacacac cccccgttcc tgttgccggt aacacaaata agtgaatcac aattgccttt
     2221 atatacttcc acaaaagcgc ctgaagacgg acctgccggg catgtatgca caacaaccgg
     2281 cagaccgtcg cctacccaac gataccagac tcctcgagga aaaacctcat ttacacagtg
     2341 ggggccgcct cctatatctg tagcggaagc caaatttgcg gttacggacg cattgcagcc
     2401 gtcaattaag gtcgctgtgg cacaaatgtc attaacaatt ggcaattgta ggcaaaatgc
     2461 aaagtcagaa tgacttgcgt caaaggcttt gaatactcga atgtgataaa tcgtattgct
     2521 ctgcagccct gtgatgacgc caccggaagg ttggtagcac cccaaagagg agaaactgcc
     2581 gcaactaccg ctcaagattt caaacccgca gacattgcct ccactcgcca gtactccttt
     2641 atataggatg tgcgaaacat tggacggggt acggaaggtg aaccatacat catgattgcc
     2701 cactgagttg caggaagata tatggtcatt gctcgctcct tgaacggtgc ccgacatggt
     2761 ggtgcatcca ccgttgggag ctatctccgg cagagcaatc gcgtcttggc agtggtcgtt
     2821 aggaggcacc ggcgttctgc ggaagcaaat atcatattgc agccctggag agtaatcata
     2881 tttgtaggcg cgcagatagt aagttgtatt ttctgccaaa ccattgatcg ttccgttgag
     2941 gctggtatag catccaatag ctgtcaaatt actacaggag cctctgaata actgtaaaat
     3001 cggagtggaa ctacccgata cattggtgat ggaataatgg agtgtcgtca tgctgggagg
     3061 gcaagtaaag gcataccaga catcgtcctt gttgccggca cagccggcta tagtagtttc
     3121 tctggctccc aaggtgttta catgcacatt ggtgcagctt ccattcgctg aaaagctgat
     3181 cacggaagcg tctgtgcaat catcgttggg cggcggcaga tgtaatgata tgttgaatcg
     3241 tccccctccg gtctcaaggg gccaactgcc caatacacgc atataataaa tatttccgcc
     3301 ctgaagattc gctattataa tttgagagcc tgaataacaa cctacggagc ttagggtggc
     3361 acaatcgccg tttaatattt gaacaccgga gactaaaaaa ctcgaattgt caatccgaca
     3421 acccaagcga gatacattgg gaggaacaat gaatcgaaac caaatatcgc cataattgct
     3481 cacggtacaa gtcggtgcgc tagagatagt agccccccgg ttgtcggcga cgataaaaat
     3541 actgtcagta tcaggttgcc atgaaggaaa gttaatagcc tgactacatt cgtcattgta
     3601 tggcatgacc atcaaacaaa gattagcaat tccaatagtg accgctgaag aagagtaaat
     3661 gcgaagatag taagttgtat tgctgttcag gtttgagatg gtgccaaagt cggctccata
     3721 acaccctaca agttggagtg tgttgcaatc ttctctaaat acttggaaat caacaccaag
     3781 agtattgtct aagtctgttt taaaatatcg cagtctggta acatctggag gcgtggtaaa
     3841 agtgtaccag atgtcgggtc tatcggttcc acaagtcatg ccgggcgtca ctgtacctcc
     3901 taaagtagtc acacgcaagg aggcacaatt gccgtctgtg gttatttcag ggaagggcag
     3961 ggcgccggag cacaaattat tagacggcct gactctgaga caaatattga aattagcaaa
     4021 cgattgcccc ccaatactgc cgtatgtata tacccgcata taataaatgg cgccttccgt
     4081 cagcccggaa atctcgcccc gggtctccga aaagcaccca aaggaaatcg ggctggcgca
     4141 attgccggcg aataactgaa accggatttg attattctca ggggggaaca actcatacaa
     4201 aagggtattt
//
LOCUS       DAORLF010000424         4183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11581_length_4183_cov_1.846899, whole genome shotgun sequence.
ACCESSION   DAORLF010000424 DAORLF010000000
VERSION     DAORLF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11581_length_4183_cov_1.846899"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /locus_tag="PK198_23085"
     CDS             <1..>2078
                     /locus_tag="PK198_23085"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF41706.1"
                     /translation="AAHIDSITRPAYRGVDRVNVMEQITIDSPQPGVYQLRVHGYLVP
                     RGPQDYVISYFLEDQELAITYPAGNEGLVPGETEVIRWDALGNTGTFTLEVSTDSMAN
                     WQTITTQIPAHQRYYEWPVPNSVTGRAFVRVRQGNTSATSAAPFNIIGLPDFQIRSDG
                     EYMARIYWPRVPGANRYTVYAMGERYMEVLGQTADTVFTFPAQINQENWYSVQAGHSS
                     GITGRRAVAKPYLHLPCQAGVQLTIMFDQFPAETRWEIRSQGGGIVSQGGPYTGFTPH
                     SVITEAVCLPYGCFQLIVYDGYNDGLCCQNGQGFYQLRTAGGTLLASGSQFTSSDTRQ
                     FCLNNPGSPLAISVFAQQHVSCYGGTNGIARVTANQGSGTYYYLWSNGATTPVISNLM
                     AGVYSVTVTDGLTQSTSSVVITQPQPIGLMMDADPVPCSGSAGGNVSASASGGTPPFS
                     YVWNTGHSGGQVSGLPAGNYSVTATDSRGCTKSDQIIVTQPGALVLSATTSPASNGNN
                     GSVHVGIGGGVAPFQFAWNNGLTTNPITGLTPGVYSVTVTDANNCSTTLSANVPGPSS
                     NFCAAQGVNTSFEHINSIQVGGLQNNSGNNGGYWDFTAMSTNMTGGATYQVALQPMYA
                     ATQYTEYWRIWIDLNRDNLFDESTELVFSAAAQGLVQGSFTLPTNYTPGPARMRIAMK
                     YGSHASPCGN"
     assembly_gap    2079..2317
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2318..2871
                     /locus_tag="PK198_23090"
     CDS             <2318..2871
                     /locus_tag="PK198_23090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF41707.1"
                     /translation="RIWIDFNRDGDFSDPGEQIFSSNQSNTVIQGTMYLPASATPGPT
                     RLRVTMRWNAYPGPCDAFAWGEVEDYTLVIANNLIGGGSSHNLRSQEGASSEAVGDIR
                     LYPNPANEKTTLYFHSKQSGTAVLEINDLSGKLLKRRQEELVPGDHLWELPIDGLVPG
                     AYLINVRTPERTYQEKLVVLQGF"
     gene            complement(2964..>3205)
                     /locus_tag="PK198_23095"
     CDS             complement(2964..>3205)
                     /locus_tag="PK198_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41708.1"
                     /translation="LTLGITIVQPFSEYIKFPSELQDDNFYQKSISLIPFRSFGLNVG
                     YRFGKVDFNKAPRQRRNSIRNDDQNRDDDGGGGGR"
     assembly_gap    3206..3218
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3219..>3936)
                     /locus_tag="PK198_23100"
     CDS             complement(<3219..>3936)
                     /locus_tag="PK198_23100"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF41709.1"
                     /translation="SFTTKLGEKYGLVTGARYERTYISGDFKTQPATFSNSYGNFLPN
                     IIISRNFKNYRTLRLSYTQRIQRPGLRNVNPFVNFNDARNISYGNPELLPELSNQVEL
                     SYNANIKGVSTNVAVFYRRTDDVIESLLKINENGVSETTFQNIGITESWGVNLFASFT
                     LKKFWTLRGNLNVNTYDGQSTLPGLNLSRQAVVWGGNINSSFSFPKDIKLEIFGNYRA
                     PRQTLQGFVPSFTMLNVGLQK"
     assembly_gap    3937..3957
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3958..>4183)
                     /locus_tag="PK198_23105"
     CDS             complement(<3958..>4183)
                     /locus_tag="PK198_23105"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF41710.1"
                     /translation="LRNERNNNLGYNLETTLQLDYMHPISPRVKMETGLKGILRNIDS
                     DFNNEYWDYDLQQFIVNDLRTDEFYYDQDVL"
BASE COUNT          934 a         1011 c         1024 g          941 t
ORIGIN      
        1 gccgcccata tagacagcat cacccgcccg gcttatcggg gtgttgaccg ggtgaatgta
       61 atggaacaaa tcaccataga ttcgccgcaa ccgggcgtgt atcagttgcg cgtacacggc
      121 tatttggtgc caagaggtcc acaggactac gtcatctcct actttttgga agatcaggaa
      181 ttggccatta cctatcctgc cggtaatgaa ggcttggtac ccggcgaaac cgaagtcatc
      241 cgctgggatg cgcttggcaa taccggcact tttacattgg aagtttctac cgacagcatg
      301 gccaactggc agaccattac aacgcaaata cctgctcatc aacgctacta tgaatggcca
      361 gtgcccaact ccgtcacagg tcgcgcgttt gtcagagtgc gccagggcaa tacctccgct
      421 acctctgctg cgccgttcaa catcatcggc ttgcccgatt ttcaaattcg aagcgatggc
      481 gaatacatgg cgcgtattta ttggccgagg gtgccgggcg ccaatcgcta caccgtgtat
      541 gctatgggag agcgatacat ggaagtattg ggccaaactg ccgatacggt attcactttt
      601 cccgctcaaa tcaatcagga aaactggtat tcggtgcagg caggccacag ttcgggcatt
      661 accggcagaa gagcggtcgc caagccttac ctgcatttgc cctgccaggc cggcgttcag
      721 ttaaccatta tgttcgacca gtttccggcg gaaacccgct gggaaatccg tagtcagggc
      781 ggaggtatcg tatcgcaagg cgggccatac accggcttta caccccattc tgtcatcact
      841 gaagcggtat gcctgcctta tggatgtttt caactcattg tatatgatgg gtataacgat
      901 ggattgtgct gccaaaacgg gcaggggttt tatcaattgc gcactgccgg cggcaccttg
      961 ttggcttcag gcagccagtt tacttcttcc gatacccggc aattttgcct taacaatccc
     1021 ggctcgccat tggccatctc tgtctttgcc caacaacatg taagttgtta cggcggcacc
     1081 aacggcatcg ccagggtaac agccaatcag ggatcgggaa cctactatta cttgtggagc
     1141 aacggcgcca ctacgcctgt gatcagcaat ctcatggccg gagtttatag cgtaaccgtt
     1201 acagacgggc ttacccaaag cacgtcctct gtagtcatta cacagccgca acctattggc
     1261 ctgatgatgg atgccgatcc cgttccctgt tcgggcagcg caggtggcaa cgtatctgcc
     1321 tcggcctccg gcggcacgcc tccgttttct tatgtttgga ataccggaca cagcggcgga
     1381 caggtatcgg gcttaccggc aggcaattat tccgtgacgg ccaccgattc cagaggatgc
     1441 accaaatcag accaaattat tgtgacgcag cccggagcgc ttgttttgag cgccactacc
     1501 agcccggcct ccaatggcaa caacgggagc gtacacgtcg gcatcggcgg cggagttgct
     1561 ccgtttcaat tcgcatggaa taatggttta accaccaatc ccatcacagg cctaacgccc
     1621 ggcgtatata gtgtgaccgt gacggatgcc aataactgta gtactacctt gagcgccaat
     1681 gttccagggc cttcctctaa tttctgcgca gcgcaaggcg tgaacaccag ctttgaacac
     1741 atcaacagca ttcaggtggg tgggcttcaa aacaacagcg gcaataatgg aggatactgg
     1801 gattttacag cgatgagcac caatatgact ggtggagcga cttatcaggt ggcgctccaa
     1861 ccaatgtatg cagccactca atataccgag tactggcgaa tatggattga cctcaaccgg
     1921 gacaacctct ttgacgaaag tacagaatta gtgttttctg ctgctgcaca gggattggtg
     1981 caaggttcgt ttacgctgcc gaccaattac acgcccggcc cggcccgtat gcgcatagcc
     2041 atgaaatacg gctcccacgc atcgccttgc ggcaatttnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gcatctggat agacttcaat
     2341 cgcgacgggg atttcagcga tcccggcgag cagattttct cctccaacca aagcaacacg
     2401 gtcattcagg gcacgatgta tctgcccgcc tcggctacgc ccggccccac tcgcctgcgg
     2461 gtgacgatgc gctggaatgc ctaccccggc ccctgtgatg cttttgcctg gggcgaggta
     2521 gaagattata cgcttgtgat tgccaataac ctgatcggcg gaggcagcag ccataatcta
     2581 agatcacaag aaggcgcttc gagtgaggca gtcggggata tccgtttgta tcccaacccg
     2641 gctaacgaaa aaaccacgct gtatttccac tcaaaacaaa gcggtacagc cgtactggaa
     2701 atcaatgact tatcgggtaa actcctgaag cgccgacaag aagaacttgt gccgggcgat
     2761 cacctgtggg agttgccgat tgacgggctt gtgccgggcg cctacctcat caacgtgcgt
     2821 acgccggaga gaacttatca ggaaaaattg gtggtactgc aaggcttttg acaaaaatgt
     2881 aaatctattg caagcacacc tcttcaacaa aaagggcctc gccgatgaag cgaagccctt
     2941 ttttttagat acgattaaag ttatcacctt ccgccgccgc cgccatcatc atcgcgattt
     3001 tggtcgtcgt tacgaatgga attgcgccgc tgccggggcg ccttgttgaa gtccaccttg
     3061 ccgaagcgat agcccacgtt gaggccaaag gaacggaatg gaataaggga aatgctcttt
     3121 tgataaaaat tgtcgtcttg cagttcggag gggaatttaa tgtactccga gaagggctgc
     3181 acaatggtga ttcccaatgt cagttnnnnn nnnnnnnncc ttttgcaggc ccacattcag
     3241 catggtgaaa gaaggtacga aaccttgcag ggtctgacgc ggcgcgcggt aattgccgaa
     3301 tatttctaat tttatatctt tgggaaaact gaagctggag ttgatattgc caccccagac
     3361 aacggcctgc ctgctcaggt tcaaaccggg caacgtactc tgaccgtcgt aagtgttgac
     3421 gttgaggttg ccgcgcagcg tccagaattt tttgagcgta aaagatgcaa aaaggttgac
     3481 gccccaggat tctgtgatgc cgatgttttg gaaagtggtt tccgatacgc cgttttcatt
     3541 gattttcagc aggctttcga tgacgtcgtc ggtgcggcga taaaacacgg ccacattggt
     3601 actcacgcct ttgatattgg cattgtaaga aagctctacc tgattggaga gttcgggcag
     3661 caattcagga ttgccatagc tgatgttgcg ggcatcgttg aaattgacaa aagggttgac
     3721 gttgcgcagg cccggccgct gaatgcgctg cgtataactg agcctgagcg tacgataatt
     3781 tttgaaattc cggctgatga tgatgttggg cagaaaattt ccataactat ttgaaaatgt
     3841 ggctggttgt gttttaaaat cgccggaaat ataggtgcgt tcatatcgcg ctcctgtaac
     3901 gaggccgtat ttttcgccca acttggtggt gaaggannnn nnnnnnnnnn nnnnnnnaca
     3961 acacgtcctg atcgtaataa aactcatcgg tacgcaggtc gttgacgatg aattgctgga
     4021 ggtcgtaatc ccagtattca ttattgaagt cgctgtcaat attgcgcaaa atgcccttca
     4081 gtcccgtttc catttttact ctggggctga tgggatgcat atagtccaat tgcagcgtag
     4141 tctcaagatt gtatccaagg ttgttgttgc gctcgttgcg cag
//
LOCUS       DAORLF010000425         4141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11735_length_4141_cov_1.890357, whole genome shotgun sequence.
ACCESSION   DAORLF010000425 DAORLF010000000
VERSION     DAORLF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11735_length_4141_cov_1.890357"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..685)
                     /locus_tag="PK198_23110"
     CDS             complement(131..685)
                     /locus_tag="PK198_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF41711.1"
                     /translation="MQKFSFLLAAMLLTAISLSAQKYFTREGKVSFFSSTPMENIEAH
                     NEQGTAVLDLSNGNIEFAVLIKAFRFEKALMQEHFNENYMESTKYPKANFKGQVLDFG
                     KINLKKDGTVTLKVKGTLTIKDVTKNVETEAVFVVAKGALSAKSAFKVAVADYNISIP
                     KLVADKIAKTVDIKVDAALAPLNK"
     gene            complement(854..1267)
                     /locus_tag="PK198_23115"
     CDS             complement(854..1267)
                     /locus_tag="PK198_23115"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41712.1"
                     /translation="MKKILLILIAIAAVAGAAGGYYLWNKPHQNMSKADAAYALEANA
                     LFMEYESDEAAANAKYLSKIVEVKGEVSQVLEGENTTVVLATENGIFGVKCELDPHSK
                     VEFPSYKAGDQVALKGECTGFLGDVVLVRCVPSER"
     gene            complement(1334..1693)
                     /locus_tag="PK198_23120"
     CDS             complement(1334..1693)
                     /locus_tag="PK198_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41713.1"
                     /translation="MKSPTILLLLSGLFLAGCYYDNEEDLYPSNCNTDSVTYSGDVAP
                     IISTNCLVCHSTAANLGNVRLEGYTALKTYVTNGKLLGAIKHQSGFSAMPQNAPKLSA
                     CNIAKIQKWVDDGALDN"
     gene            complement(1738..2481)
                     /locus_tag="PK198_23125"
     CDS             complement(1738..2481)
                     /locus_tag="PK198_23125"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF41714.1"
                     /translation="MNAVSRFSIAGWCIALTILLSSALLPGCKHEPFATDITDPGDTT
                     NVNPGTPCDPQVVYFETQVLPILQSNCALSGCHDEASHEEGIILTSFTRVMQTADIRP
                     GNASRSDLYEVLVETRADKKMPPPPRAGLTQAQISLVANWINQGAKNLRCDAGAGPCT
                     TTDRSWAQHIRPIIQTYCLGCHSGTTPGAGLNFSTHTGVAAATTNGRLYGAINHQQGY
                     AAMPPTGTKMPECQIMQIKSWIDAGAPNN"
     gene            2661..3218
                     /locus_tag="PK198_23130"
     CDS             2661..3218
                     /locus_tag="PK198_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF41715.1"
                     /translation="MKNFLLPIFIAIGLCLSLESVAQTGIFKTTSGRITFRSEAPLEL
                     IEAASKELSGVLDAARRTFAFSVPMQSFQGFNSALQREHFNENYMESTRFPKAVFTGK
                     IIESTDLTQPGEYVVRAKGKLSLHGVEQERIIKVNIVTDKKGMTAHAQFSVLLEEYNI
                     SIPRVVYQKIAEEIQISVEAVLAKS"
     gene            3280..>3904
                     /locus_tag="PK198_23135"
     CDS             3280..>3904
                     /locus_tag="PK198_23135"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF41716.1"
                     /translation="MFKKLEPGGAFSELRIGAVASDQHGYLWLGADAGLLRFDGIRAE
                     QPQVSDPQLTKNKVTALYTDRNGLLWAGFKNGLIARLGSDGILRRWEPEEGLPSAPVT
                     GFAESNDGTLWMATYGEGLYRYDGHHIYNINLDDGLPSNDVYCIARDAGGRIWAGTDA
                     GIAVCSFVKNQKKIEVLSTEQGLPDPIVTALLSDERGQIWAGMHDGGV"
     assembly_gap    3905..3921
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3922..>4141
                     /locus_tag="PK198_23140"
     CDS             <3922..>4141
                     /locus_tag="PK198_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41717.1"
                     /translation="MYHPAAAQFFCMEMPWSNGVISALAMLPGRELWIGTEGEGLFRY
                     ELNGGRLSRINGDAGLPRSKITALHFGS"
BASE COUNT          895 a         1050 c         1144 g         1035 t
ORIGIN      
        1 atctaatcct ttggcctaaa gaaaatgaac actttatttt tgccccacta ctaacggaga
       61 aaacgaacaa agcctgcccg ttggaatgat ccttttcggg caggctttga tacaatcggc
      121 gtcggagcaa ttatttattg agcggagcca gagcggcgtc cactttgatg tccacggttt
      181 tggcaatttt gtcggccacc aatttcggga tgctgatgtt gtaatcggca acggccactt
      241 tgaaggccga cttggcgctg agcgctcctt tggccaccac aaaaacggct tcggtttcca
      301 cgtttttggt cacgtctttg atggtgagcg tgcctttcac tttgagggtc acggtgccgt
      361 cttttttcag atttattttg ccaaaatcaa gcacctgtcc tttgaaattg gccttgggat
      421 acttggtgct ttccatgtag ttttcgttga agtgctcctg catgagggcc ttctcaaagc
      481 ggaatgcttt gatgagtacg gcaaattcga tgttgccgtt actcaggtcc aatacggcgg
      541 tgccttgctc gttgtgcgct tcgatgtttt ccatcggcgt gctggaaaag aaggacactt
      601 ttccttcgcg ggtaaagtac ttctgcgccg agaggctgat ggctgtcagc agcatggctg
      661 cgaggagaaa tgagaatttt tgcatggttt attgagcgtt gattgtttac ttattcggtt
      721 ggcggtttct cagtgggcag tatgcggtat tcggtcggcg gctctaagag tagagcgttc
      781 agtcggcagt gcaaagcgta ggatgcggtg ggcagtcgcg agaggaaccg cccaccgccc
      841 accgcctacc ggcttaccgc tcagaaggca cgcagcgcac cagcaccaca tcgcccagaa
      901 acccggtaca ctcgcctttc agagctacct gatctcctgc tttgtaactt ggaaactcga
      961 ctttgctgtg cgggtccaat tcacatttga cgccgaaaat gccgttttcc gtggccagta
     1021 ctacggtggt gttttcaccc tccagcactt gcgatacctc gcctttcact tccacgattt
     1081 tggagaggta tttagcattg gcggcagcct catcgctttc gtattccatg aataaagcat
     1141 tggcttccag cgcgtaagcg gcatctgcct tcgacatgtt ttggtgcggc ttgttccaca
     1201 ggtagtaccc gccggctgcg cctgccaccg ctgctatggc gatgagaatg agcagtattt
     1261 ttttcatctg attgactttt tttggatgca ggaacggtca caagctacag gcttcaagcc
     1321 acaagctgtt gtcttaatta tccaatgcgc cgtcgtccac ccatttctgg attttggcaa
     1381 tgttgcaggc cgagagtttc ggtgcgtttt gcggcatggc cgagaaaccc gattgatgct
     1441 tgatggcgcc caaaagcttg ccgttggtca cataggtttt cagagcagtg tagccttcca
     1501 atcgcacgtt gcccaaattg gcggccgtac tgtggcacac taaacaattg gtagaaatga
     1561 tgggagcaac gtcgccggaa tacgtcacgc tgtcggtgtt gcaattggaa ggatacaaat
     1621 cctcttcgtt gtcataatag cagccggcaa ggaacaagcc ggacaaaagc agcaggatgg
     1681 tcggtgattt cattgatcgt tctgtttttt ggcacagtaa acgcattttc ctgcgcatta
     1741 gttattgggc gcccctgcat ctatccacga ttttatctgc atgatctggc actccggcat
     1801 tttcgtgccg gtgggcggca tggctgcata gccttgttgg tgattgatgg cgccgtacag
     1861 cctgccgttt gtggtagcgg cggccacgcc tgtatgtgtg ctgaaattca aacccgcgcc
     1921 gggagtcgtt ccgctatggc aacccaaaca ataggtctga ataatgggcc gtatatgctg
     1981 cgcccagctt ctgtcggtag tggtgcaggg gccggctccg gcatcgcagc gcagattttt
     2041 ggcgccctga ttgatccagt tggcaacgag gctgatctgt gcctgagtga ggccggctct
     2101 gggcggcggc ggcatttttt tatcggctct ggtttctacc aatacttcgt acagatcgct
     2161 gcgggaggca ttgccgggcc ggatgtcggc ggtttgcatc acgcgggtga aggaggtaag
     2221 gatgatgcct tcctcatgag atgcctcgtc atggcagccg ctcagggcgc aattggactg
     2281 caaaatgggc agcacctgcg tttcaaaata cactacctga ggatcgcagg gcgtgccggg
     2341 gttgacgttt gtggtgtcgc cggggtctgt aatgtcggtg gcaaaaggct cgtgtttgca
     2401 gcccggtaac agtgcagaag agagaagtat ggtaagcgct atacaccaac ccgctatgga
     2461 aaatctggaa actgcgttca tgcgttcaat tttcaaaact cgctgcaaac ataagcccgg
     2521 cgccggcgta cccgcaaata agataaaccg aaccgggaaa aacggagtct gaaccggggc
     2581 cagccggttt cagcctgcca ttttcccgat tgagtcaaca tattttttcc cgggctgccg
     2641 aatttgctca aatttggcct atgaaaaact tcttgcttcc catttttata gccattgggc
     2701 tgtgcctttc tttggaaagt gtcgcacaaa cgggcatttt caaaaccact tccgggcgca
     2761 ttacctttcg ttccgaggcc ccgctcgaac tcatagaagc ggcctccaaa gaactcagcg
     2821 gcgtgctgga tgctgcccgg cgcacttttg ctttcagcgt gcccatgcag tcgtttcagg
     2881 gttttaacag cgccttgcaa cgcgagcatt ttaacgaaaa ctacatggaa agcacccgtt
     2941 ttccgaaggc tgtctttact ggaaaaatta tagaatcaac cgatttgaca cagcctggcg
     3001 aatatgtcgt tcgcgccaaa ggcaaactct ctttgcacgg cgtcgagcag gagcgcatca
     3061 tcaaggtgaa cattgtgacc gataaaaaag gcatgacggc gcacgcccag ttctccgtac
     3121 tgcttgaaga atacaacatc tccattccca gagtggtgta tcaaaaaata gccgaagaaa
     3181 tccagatttc cgttgaagct gttcttgcca aatcatagtc gggcgctttt gccggcctgc
     3241 tgcctgttgt gtgcagtgtc tgtcggtgcg caaattccgg tgttcaaaaa actggaaccc
     3301 ggcggcgctt tttccgaact ccgcatcgga gcagttgcct ccgaccaaca cgggtatctt
     3361 tggctgggtg ccgatgccgg actgttgcgc tttgacggaa tacgggcgga acaaccacaa
     3421 gtatctgatc ctcagttgac aaaaaacaaa gttacggcac tctatacaga tcgaaacgga
     3481 ctgctttggg cgggcttcaa aaacggcctc atcgctcgtt tgggcagcga cggcatcttg
     3541 cgaaggtggg agccggaaga agggctgcct tcagcgccgg tcaccggatt tgcggaaagc
     3601 aacgacggca cactttggat ggccacctac ggcgagggct tgtatcgcta cgatggacac
     3661 catatttaca acatcaacct cgacgacgga ttgcccagca atgatgtgta ttgcatcgcg
     3721 cgagatgccg ggggccgtat ctgggcgggc accgatgccg gaattgccgt ttgcagcttt
     3781 gtgaaaaatc agaaaaaaat agaggtgctg agcacagaac aaggcttgcc cgaccccatt
     3841 gtgacggcgc tgctctctga tgagcggggg caaatctggg caggcatgca cgacggcggc
     3901 gtggnnnnnn nnnnnnnnnn ncgatgtacc acccggcggc agcgcagttt ttttgcatgg
     3961 aaatgccctg gtcgaacggc gtaatctcgg ctttggccat gctgccgggc cgcgaattgt
     4021 ggatcggaac ggagggcgag ggcttgtttc gttacgagct gaatggcggc cgcctgagta
     4081 gaatcaatgg cgatgccggc ctgccccgct ccaaaattac agccctgcac tttggcagtg
     4141 a
//
LOCUS       DAORLF010000426         4139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11743_length_4139_cov_2.293340, whole genome shotgun sequence.
ACCESSION   DAORLF010000426 DAORLF010000000
VERSION     DAORLF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11743_length_4139_cov_2.293340"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK198_23145"
     CDS             complement(<1..489)
                     /locus_tag="PK198_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRF41718.1"
                     /translation="MENKQNQSNVVLAIDIGTTKVCAIAGRKNEYGKIEVIGISKVES
                     EGVLRGVVTNIEKTVNAIAEAVVAVRRQTGIDFHTVHVGIAGQHIKSLQHRGMLMRDN
                     DHTEISRRDIDRLINDMYKLVLPPGDKILHVIPQEYTVDNEQGITDPIGMSGVRLEAN
                     FHI"
     gene            complement(559..1341)
                     /locus_tag="PK198_23150"
     CDS             complement(559..1341)
                     /locus_tag="PK198_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41719.1"
                     /translation="MKSKEELVASSKKFGWIAGLLLLAMIVLSAIGKKESSPATEVVV
                     EVAPLEGGKLLINEKDVLEMVGTVYGYTLEGRPTGQIDLQRIEKMLENDPFVLNADVF
                     LNARNSVNIRVQQREPVLRIIDNNALNYYLDKDGVKMPVSKRAAAHVMVATGNIPPHD
                     PGFLERKRHLLKEVFLLSQMLREDAFFNAMIEQIHVSNQGEMTLVPKIGDQKIFFGKL
                     ADADKKLEKLRIFYQEGMPYEGWRKYKSIDVRYAGQIVCKKR"
     gene            complement(1331..>2659)
                     /gene="murC"
                     /locus_tag="PK198_23155"
     CDS             complement(1331..>2659)
                     /gene="murC"
                     /locus_tag="PK198_23155"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRF41720.1"
                     /translation="GGIGMSALARYFHGRGVEVFGYDKTETPLTRRLSEEGMNIHYHD
                     APASIPAGIDLAVFTPAVPADLGELQHFRDSGLPLKKRAEVLGIISRGMKTIAIAGTH
                     GKATTSSLTTWLLRSSGIDCSAFLGGIVRNFGSNFVEGRSEWVVVEADEYDRSFLQLD
                     PDVAVILSMDADHLDIYGDRESMVETGYKAFAAKIKSGGSLFVQHRWAGELPDAQTFG
                     IENGQYRAENIRVEDGFFVFDYLSADLAMRGLRLAMPGRHNVENATAAISAALCAGAD
                     EAGIRRGLETFGGIQRRFEILFRSDAVVYVDDYAHHPSELEAVIGAAREFFPGRKLTG
                     VFQPHLYTRTRDFAEGFAQALDRLDEVILLDIYPARELPIAGVDSQMIAAMMQRAQVT
                     LCSKSELMNELKGRHIEVLLTLGAGDIDTFTEPIRQWLSAGANNSQGHEK"
     assembly_gap    2660..2718
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2858..3982)
                     /gene="murG"
                     /locus_tag="PK198_23160"
     CDS             complement(2858..3982)
                     /gene="murG"
                     /locus_tag="PK198_23160"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRF41721.1"
                     /translation="MQTTKHTTSTKAPRILISGGGTGGHVFPAIAIADAVKKQAPEAE
                     ILFVGALGKIEMEKVPQAGYRIEGLWISGFQRKLTLRNLLFPIKLLSSMWKARRIVRS
                     FRPDVAVGVGGFASGPTLENACRMGIPALVQEQNSYAGVTNRLLANKVQRVCVAYEGM
                     DRFFPADKIVLTGNPVRSDLARLTATKAEAAAHFQLDADKRTVLVFGGSLGALALNEA
                     MAAGKEQMAAHPDVQFLWQCGKLYIERFSQCEAAQLPNVRVLPFVDRMDLAYALADVI
                     VGRAGALTISELCLVGKPAILIPSPNVAEDHQTKNAQALADKGAALMIRNSEAMARLV
                     PEALELLNDEARCRQLSANIRTLAKPNAAEEIAREVLGLM"
BASE COUNT          816 a         1160 c         1138 g          966 t
ORIGIN      
        1 gatgtggaaa ttggcttcga ggcgcacgcc cgacatgccg atggggtcgg tgatgccctg
       61 ctcgttgtcc acggtgtatt cctgcgggat gacgtgcagg attttgtcgc cgggaggcag
      121 cacgagcttg tacatatcgt tgatgaggcg gtctatgtcg cggcggctga tctcggtgtg
      181 atcgttgtcg cgcatgagca tgcctctgtg ctgaaggctt ttgatgtgtt ggccggcaat
      241 gcccacatga acggtgtgga agtcaatgcc ggtttggcgg cgcacagcca cgacggcttc
      301 ggcaatggcg ttgaccgttt tttcgatgtt ggtgacaaca ccgcgcaaaa cgccctcgga
      361 ttccaccttg ctgatgccga tgacttcaat tttgccgtac tcgtttttgc ggccggcaat
      421 ggcgcacact ttggtggtgc cgatgtctat ggcgagcacc acgttggatt ggttttgttt
      481 gttttccatg ttacaaagca ttttttaatt gacgcttgcg ttcgtgacgg aggcctgcgt
      541 cgggttaaat ataaatggtt aacgtttctt acaaactatc tggccggcat agcgcacgtc
      601 aatggacttg tacttgcgcc agccctcgta gggcatgcct tcctggtaaa aaatgcgcag
      661 cttttcgagc tttttgtcgg cgtcggccaa tttgccgaag aagattttct ggtctccgat
      721 tttgggtacc aaggtcatct cgccctgatt ggagacgtga atctgctcaa tcatagcgtt
      781 gaaaaacgca tcttcgcgca gcatttgcga aagcagaaag acctctttga gcaggtgccg
      841 tttgcgctcc agaaagccgg gatcatgcgg cggaatattg cccgttgcca ccatcacatg
      901 cgccgcggcc cgcttcgaca ccggcatctt cacgccgtct ttgtccaagt agtaattcag
      961 cgcgttgttg tctatgatgc ggagtaccgg ttcgcgttgc tgtacgcgta tgttgacgga
     1021 attgcgggcg ttgagaaaca catcggcatt gagtacaaag ggatcgtttt ccaacatttt
     1081 ttcaatgcgt tgcaggtcta tctgcccagt cggacggcct tccagcgtgt agccatatac
     1141 cgtgccgacc atctccagca cgtccttttc attgatgagc aacttgccgc cctccaatgg
     1201 cgccacctcc acaactacct ctgtagccgg actggattct ttttttccaa tggcggaaag
     1261 gacgatcatg gccagcagta agagacctgc tatccaaccg aactttttgg aggatgctac
     1321 caattcttct ttacttttca tggccttgcg agttgtttgc tccggcgctc aaccactgcc
     1381 ggatcggttc tgtaaaagtg tcaatatctc cggcgccaag ggtgagcaat acctctatgt
     1441 ggcggccttt caattcgttc atcagttcgc ttttgctgca aagcgtcacc tgtgcgcgct
     1501 gcatcatggc ggcaatcatc tgactgtcaa cgccggcaat gggcagttcc cgcgccgggt
     1561 agatgtccaa cagaatcacc tcgtccaaac ggtcgagcgc ctgtgcaaag ccctctgcaa
     1621 aatcgcgggt gcgggtgtag aggtgcggct gaaacacacc tgtgagcttc ctgccgggaa
     1681 agaactccct cgctgccccg atgacggcct ccaattcgga gggatggtgc gcgtagtcgt
     1741 ccacatacac cactgcatcg ctgcggaaga ggatttcaaa gcggcgctga atgcctccaa
     1801 acgtctccaa tccgcgccgg atacctgcct cgtctgcgcc ggcgcaaagg gcggccgaaa
     1861 tggcggcggt ggcgttttct acgttgtggc ggcccggcat ggcaagtcgc aaaccgcgca
     1921 tggcaaggtc ggcggagagg taatcgaaca cgaagaagcc gtcttccacg cggatgtttt
     1981 cagcgcggta ctgcccgttt tcgataccga aagtttgtgc atcgggtagt tcgccggccc
     2041 agcggtgctg tacaaaaagg cttccgccgg atttgatttt ggccgcaaag gctttgtagc
     2101 cagtctcgac catgctttca cggtcgccgt aaatgtccaa atgatcggca tccatcgaga
     2161 ggatgacggc cacatcggga tcgagttgaa ggaaggagcg gtcgtactca tcggcctcca
     2221 ccactaccca ctcgctgcgg ccctccacga agttggaacc gaagttgcgc acaatgccgc
     2281 ccagaaacgc gctgcaatct atgccgctgc tgcgcaagag ccaggtggtg agcgaggagg
     2341 tggtggcttt gccatgcgtg ccggcgatgg cgatggtttt catgccccgg ctgatgatgc
     2401 ccaatacctc ggcgcgtttt ttcagcggta ggccgctgtc gcggaaatgc tgcaattcgc
     2461 ccagatcggc gggtactgcg ggcgtgaaca cagccagatc aatgcctgcc ggaatggagg
     2521 ccggcgcatc gtggtagtga atgttcatgc cttcctcgct gagtcggcgg gtgagcggcg
     2581 tttctgtttt gtcgtaacca aagacctcca cgcctcgtcc atggaaatag cgggccagag
     2641 cgctcatgcc gatgccgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnac aatttaatta tttcggcagt tacgagccac gagctgcgag
     2761 tttgagtccc caaaaggaca aactgtttac aaaaaaagca atcagcccct ctcgattggc
     2821 aatcagctca tcagcacatt gccacatcag cacattatca catcagcccc agcacctccc
     2881 gcgcaatctc ctcggcagca ttgggtttgg ccaaagtgcg gatgttggcg gagagttggc
     2941 ggcaacgcgc ttcgtcgttc aaaagttcca gtgcttccgg taccaggcgg gccattgcct
     3001 cgctgttccg aatcatcaga gcggcgcctt tatcggccaa agcctgggcg tttttcgtct
     3061 gatggtcttc ggccacgttg ggcgatggta tcagtatggc aggctttcct accaaacaca
     3121 attcagagat ggtgagcgcg ccggctcttc ccacaatcac atcggccagc gcgtaggcaa
     3181 ggtccatgcg atctacgaaa ggcagcacgc gcacgttggg caattgggcc gcctcgcatt
     3241 gcgaaaagcg ctcgatgtac agtttgccgc attgccatag aaactgtacg tcgggatgcg
     3301 cggccatctg ttctttgcct gccgccatcg cttcgttgag cgccagagcg ccgaggcttc
     3361 cgccaaatac gagtaccgtg cgtttgtcgg catccaactg aaaatgagcc gccgcctccg
     3421 ctttggtcgc cgtcagccgg gccagatcgg agcgcaccgg attgcctgtc agcacgattt
     3481 tatcggcagg aaagaagcga tccatgccct cgtaggccac gcacacgcgc tgcactttgt
     3541 tggccagcag gcgattggtg acgccggcgt acgagttttg ctcctgcacc aaggccggaa
     3601 tgcccatccg acaggcgttt tccaaagtgg ggccgctggc aaaaccgccc acgcctaccg
     3661 ccacatcggg ccggaagctg cgcacaatgc ggcgcgcttt ccacatgctg ctgagcaact
     3721 tgatgggaaa gagcaagttg cgcaaagtga gtttcctttg aaacccgctg atccacaagc
     3781 cctctatgcg atagccggcc tgcggcactt tttccatttc tattttcccc aatgctccca
     3841 caaacagtat ctctgcctcc ggcgcctgct tttttactgc atcggcaatg gcaatggccg
     3901 gaaagacgtg ccccccggta ccacccccgc tgatgagtat gcgcggcgct ttggttgatg
     3961 ttgtatgttt ggttgtttgc attttctttt agattcaaaa tttcgggttc aaggtttcag
     4021 gttcaaggtt tcgggttgga accgcaaacc gccgaccgcc tactgcaaac cggcaccccg
     4081 ccccctactc cgccgtcgcc tctatgtgtt tgctcacgct caggatcatc ccaaaggcg
//
LOCUS       DAORLF010000427         4138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11746_length_4138_cov_2.499388, whole genome shotgun sequence.
ACCESSION   DAORLF010000427 DAORLF010000000
VERSION     DAORLF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_4138_cov_2.499388"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1309)
                     /locus_tag="PK198_23165"
     CDS             complement(254..1309)
                     /locus_tag="PK198_23165"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41722.1"
                     /translation="MLETNRKKLTDGEAEVLAVQDQIDRLRVEVTQGIQVRKERESTL
                     SEAEQAYFKTRGEINQIEDQLRKVGKNRQDAQILINNLKDKFTEAKFDISSVAQRLRI
                     EFEVDINEIINTQKPSDTPQEELELRVERTKSRLDNYGEINPMAVEAYDEMKERHDTI
                     SVQRDDVLKAKESLLQTMQEIEATATSQFLEAFDRARLHFIEVFRSLFTEDDTCDLIL
                     LDPNNPLDSKIEIVARPKGKRPQTISQLSGGEKTLTATALLFALYLLKPAPFCIFDEV
                     DAPLDDSNINKFNRIIKKFSKESQFIIVTHNKLTMAAVDTIYGVYMAEQGVSGVSQVD
                     FREFELSGEIITATSKG"
     gene            complement(1334..3814)
                     /gene="smc"
                     /locus_tag="PK198_23170"
     CDS             complement(1334..3814)
                     /gene="smc"
                     /locus_tag="PK198_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRF41723.1"
                     /translation="MRLKTLEIKGFKSFAAETAIHFNEDVIGIVGPNGSGKSNIVDAI
                     RWVLGEQKSSELRLDQMTSVIFNGTKNRKQGNVASVSLTFENTRNLLPTEYNTVTITR
                     LLYRSGESEYRLNNVPCRLKDITTLLMDTGIGSNSYAIIALGMVDDILNDKDNSRRRM
                     FEQAAGISKYKARKRETLSKLESASADLDRIEDLLFEIDNNLKTLEKQAKRTKRYFEL
                     REEYKVMSIDLALVKVAVLKVRYNALQKQLQEEEDRYRQLEIEVHRLESTLEQEKKTN
                     LDKEKALSEKQRDLNELTGKIRGLENELRMQEQRLQFIHNNEARLREEIKAAEARSAQ
                     LHADVEHYRSELNAEKRVEARLQMELETAEAELVHIRQSHGSIKNTLDEVVRRQQLIE
                     REIFELEKQKAISANQLQNLEADIQRTDTAMQLRKTEVENLQTQMDALEQREREQQAV
                     ATQLEQDEAKRQTDLRQAETELEELTKKLTKLNRELDAKRNEFKLTKSMIENLEGFPE
                     SIRFLSSSKDWKKDTPLLSDIIYVKEDYRVAIENYLEPYLNYYVVQSLEDAFQAIRLL
                     SKSQKGKANFFILDAFKDYAAPIALLPDTTRATDLVETDPAYRNLCDFLLEKVLLTAK
                     DEHQLVAADPGMVLLSQSGQYIQRRYSVSGGSIGLFEGKRIGRKKNLEMLETAIRKLE
                     TEEAKLSAAFAAAKGKIEQLKSRRGSELIQQERNKLHQIVQQKVSLATRLESFDSFIR
                     ANVAQQQESEAKISALREAITGIDGMLTDKEQVLAEAKSNISGTDSSYRQAAEQLSQA
                     SAAFNDKNIHFIRQQNKVTALQRELGFR"
BASE COUNT          703 a         1005 c         1296 g         1134 t
ORIGIN      
        1 tccagcagat gggtggtgaa gttgcggctt cccgggcgac tgaagcacgc cggtactgca
       61 gtgcgcgatg gccgccgcgc cagggacagt agcggatgcg ctgagcggct ccgtagcgcg
      121 gcggttttat accccgcaca gcggagccgc tcagcgcatg gagcggatgg cccgaccccg
      181 ccgcctgtgg ccactcgcct gagcgaagca gctgggtgtg gccggtagtg cggggtggcg
      241 cccacacctt gctctacccc ttcgacgtag ccgtaatgat ctctcccgaa agctcgaact
      301 cccggaagtc cacctgactg acgccggaga cgccctgctc ggccatgtaa acgccgtaga
      361 tggtgtccac ggcggccatg gtgagtttgt tgtgggtgac gatgatgaat tgggattctt
      421 tggagaattt cttgatgatg cggttgaatt tgttgatgtt gctgtcgtct aagggggcgt
      481 ccacctcgtc gaagatgcag aagggcgcgg gcttgaggag gtagagggcg aacaaaagcg
      541 cggtggcggt gagggttttt tcgccgccgg aaagctgact gatggtttgc gggcgcttgc
      601 ctttgggccg ggctacgatt tcgattttgg aatctaaggg gttgttggga tcgaggagga
      661 tgaggtcgca ggtgtcgtct tcggtgaaga ggctgcggaa tacctcgatg aagtgcaggc
      721 gggcgcggtc gaaggcttcg aggaactggc tcgtggcggt ggcttcgatc tcctgcatgg
      781 tttgcagcag cgattctttg gctttcagca cgtcgtcgcg ttgtacggag atggtgtcgt
      841 ggcgctcttt catttcgtcg tacgcctcca cggccatggg gttgatttcg ccgtaattgt
      901 ccaagcggct tttggtgcgc tctacccgca gttcgagttc ttcctgcggg gtgtctgagg
      961 gcttttgggt gttgatgatt tcgttgatgt ccacctcaaa ttcgatgcgg aggcgctgtg
     1021 ctaccgagct gatgtcgaac ttggcttcgg tgaatttgtc tttgaggttg ttgatgagaa
     1081 tttgagcatc ctggcggttt ttgcccactt tccggagctg atcttctatc tgattgatct
     1141 cgccgcgggt tttgaaatat gcctgctcgg cctccgagag cgtggattcc cgctctttgc
     1201 gcacctgaat gccctgcgtg acctctacgc ggaggcggtc tatctggtct tgtactgcaa
     1261 gcacttccgc ctcgccgtcg gtcagttttt ttcgattggt ttccaacaaa acggcggcct
     1321 cttcgatctg cttttagcgg aagccaagct cgcgttgcag ggcggtgacc ttgttttgct
     1381 gccgaatgaa gtggatgttt ttgtcgttga aggccgccga cgcctgactg agttgctcgg
     1441 ctgcctgccg gtaggagctg tcggtgccgg agatgttgga tttggcttcg gccaatactt
     1501 gttctttgtc cgtcaacatg ccgtcaatgc cggtgatggc ttcgcgcagg gcgctgattt
     1561 tggcttccga ttcctgctgt tgggccacgt tggcgcggat gaagctgtcg aaactctcca
     1621 aacgcgtagc caaagagacc ttttgctgca cgatctggtg gagtttattg cgctcttgct
     1681 gaatgagttc tgagccgcgt cgcgatttga gttgctcaat tttgcctttg gcggcggcaa
     1741 aagcggcgga gagtttggcc tcctcggtct ccaacttgcg aatggccgtt tccagcattt
     1801 ccaggttctt tttcctgccg atgcgcttgc cctcgaacag cccgatggag ccgcccgata
     1861 cgctgtaccg tcgctgaatg tattgcccgc tttgcgaaag cagcaccatg ccgggatcgg
     1921 cagcaacgag ttggtgctcg tctttggccg tgaggagcac tttttcgagc aggaaatcgc
     1981 agaggttgcg gtaggccgga tcggtttcta ccagatcggt ggcgcgggtg gtgtcgggca
     2041 gcagggcgat gggcgccgca tagtctttga aggcgtcgag gatgaaaaaa ttggccttgc
     2101 ctttttgcga tttgctcagc aggcgaatgg cctgaaacgc gtcttcgagg ctttgtacca
     2161 cataataatt gaggtacggc tctaagtagt tttcgatggc cacgcggtag tcttctttca
     2221 cataaatgat gtcggacagc agcggggtat cttttttcca gtctttggaa ctgctcaaaa
     2281 aacggatgga ttcggggaat ccttcgaggt tttcgatcat gctcttggtg agcttgaatt
     2341 cgttgcgttt ggcgtcgagt tcgcggttga gtttggtgag ttttttggtc agctcctcca
     2401 attcggtttc agcctgccgg aggtcggttt ggcgcttggc ctcatcctgc tccaattgcg
     2461 tggcgacggc ctgctgctcg cgctcgcgtt gttcgagggc gtccatctgg gtttgcaggt
     2521 tttctacctc ggttttgcgc agttgcatgg ccgtgtcggt gcgctgaatg tcggcttcca
     2581 aattttgcaa ctgattggca ctgatggctt tttgtttttc caactcaaag atttcgcgct
     2641 cgatgagttg ctgccggcgc accacttcgt cgagggtgtt tttgatggag ccgtggcttt
     2701 ggcgaatgtg gacgagttcg gcctcagcgg tttcgagttc catttgtagg cgggcttcca
     2761 cgcgtttttc ggcgttgagt tcactgcggt agtgctctac gtcggcgtgg agttgggcgc
     2821 ttcgggcttc tgccgctttg atttcctcgc gcaggcgggc ctcgttgttg tggatgaatt
     2881 gcaggcgttg ttcctgcatg cgaagctcgt tttcgaggcc gcgtattttg ccggtcagtt
     2941 cgttgaggtc gcgctgtttt tcggagaggg ctttttcctt gtccaagttg gtttttttct
     3001 cctgttccag cgtggattcg aggcggtgta cctcgatttc gagttgccgg tagcggtctt
     3061 cttcctcctg aagttgtttt tgcaaggcgt tgtaccgcac tttcagcacc gccactttca
     3121 ccagggccag atcaatgctc atgaccttgt attcctcgcg cagttcgaag tagcgtttgg
     3181 tgcgttttgc ctgtttttca agggttttga ggttgttgtc aatctcaaaa aggaggtctt
     3241 cgatgcggtc gaggtcggcg gaagcgcttt cgagcttgga gagcgtttcg cgtttgcggg
     3301 ctttgtattt ggagatgccg gcggcctgct cgaacatgcg ccggcgcgag ttgtctttgt
     3361 cgttgaggat gtcgtccacc atgcccagcg cgatgatggc gtaggaattg gagccgatgc
     3421 cggtgtccat cagcagggtg gtgatgtctt tgaggcggca gggtacgttg ttgaggcggt
     3481 attcgctttc gccgctgcgg tacaacaagc gggtgatggt gacggtgttg tactcggtgg
     3541 gcaggaggtt gcgggtgttt tcaaaggtga gcgaaacgga ggccacgttg ccctgtttgc
     3601 ggtttttggt gccgttgaaa atgacgctcg tcatttggtc caagcggagt tcgctgcttt
     3661 tttgctcgcc caaaacccag cggatggcgt ccacgatatt ggatttgcct gaaccgttgg
     3721 ggcctacgat gcctattaca tcttcgttga agtgaatggc cgtttcggcg gcaaagcttt
     3781 tgaagccttt gatttctaaa gtttttagtc gcataaggcc gcaaaaatag gaaaaacgga
     3841 gctatgcccc tctccgagca gcgcaaatca taaaaatcat aaagatcagc gtcggaaaaa
     3901 acaggtactc gccaagtcag ggcttaaccg gaagttacgg cctgacctcg gttcggggta
     3961 gtgaaggggt gactcagcaa gtttcagcgg gtcagtcacc ccttcactaa gccagagaac
     4021 ccccaaaaca aggcccggtt tctgacgctg aaagacgcag gttcttatga tcgttatgcc
     4081 cccctccgag cagcgcaaat cataaaaatc ataaagatca gcgtcggaaa aaacaggt
//
LOCUS       DAORLF010000428         4137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11752_length_4137_cov_1.965703, whole genome shotgun sequence.
ACCESSION   DAORLF010000428 DAORLF010000000
VERSION     DAORLF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_4137_cov_1.965703"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    232..383
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <384..713
                     /gene="cutA"
                     /locus_tag="PK198_23175"
     CDS             <384..713
                     /gene="cutA"
                     /locus_tag="PK198_23175"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRF41724.1"
                     /translation="YVTHSSEEEARRIANAAVSARLAACANLFPIQSSYWWDGKIESG
                     AEWVSVLKTAPYLEQRMEEFIERLHPYETPCILRWEVRANEAYENWILASVLRKEEVD
                     GAKVDRF"
     gene            complement(749..>1349)
                     /locus_tag="PK198_23180"
     CDS             complement(749..>1349)
                     /locus_tag="PK198_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRF41725.1"
                     /translation="AGIRSAELEISGDFAYGLLKGENGVHRLVRVSPYNAQGKRMTSF
                     SSVFVHPMVDERIEVEVNPADLEWDTFRSSGAGGQHVNKTESAVRVRHLPSGLVVECQ
                     QERSQHQNRDKAMQMLKSRLYELELKKRLEARDKIESGKMKNEWGSQIRSYVLDDRRV
                     KDHRTGHQTSQTDAVLDGDLDDFLKAFLMYHGEPVPEDD"
     assembly_gap    1350..1405
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1406..1851)
                     /locus_tag="PK198_23185"
                     /pseudo
     CDS             complement(1406..1851)
                     /locus_tag="PK198_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762823.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(2008..2850)
                     /locus_tag="PK198_23190"
     CDS             complement(2008..2850)
                     /locus_tag="PK198_23190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF41726.1"
                     /translation="MRIFLFAALGLLFLASGCNKPKEQVTASGFRYTVHVDKDGDVPA
                     PGDIVFFHATVRRDGSDSIYYDTRSSGEDMPSLQIPTEEIPGQPASPVLELVRVLSIG
                     DSATVVYQVDTMAQKPMGFEDAKELYYDIVIVDIKKMSDLKAEEDRVAALVNEFAKTV
                     SDGKQPEGMKSTESGLQYVILKQGDGAAAEPGKDVQVNYYGALPDGTLFDTSLSRGTP
                     LPFKLGDPGLIPGWTEGVDLLNQGGQAILYIPSELGYGEAGAPPVIPANSKLVFYIEV
                     VSVK"
     gene            complement(2889..3896)
                     /locus_tag="PK198_23195"
     CDS             complement(2889..3896)
                     /locus_tag="PK198_23195"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRF41727.1"
                     /translation="MENIHELKAQLASPKDIVIISHRNPDGDAIGSSLGLYHYLLSQG
                     HAVHVVLPSEYPDAFLWMPGIQNIIVFDSDQERTREVCERAQIIFCLDFNSLDRIDKV
                     GEIIQPLNSVKVLIDHHLYPEPFADYVMSDTTASSTAEMVFDFIVLMGHRPDISRATA
                     DCVYTGIVTDTGSFKFSTSPKLFRIVAELLELGVDDYKLQDLIFNSLDEKQLRLLGHC
                     LYNRMEILPEYRTGIITLTKEDYEHFDIVRGETEGIVNYLLKMRDITMAAFITEQPSI
                     VKISLRSKGDFSVQDICRKHFKGGGHRNASGGFSFQPLKVTLRKFKELLPEYKDALNN
                     Y"
     gene            4021..>4137
                     /locus_tag="PK198_23200"
     CDS             4021..>4137
                     /locus_tag="PK198_23200"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRF41728.1"
                     /translation="MAGNKTFTMIKPDAVESGHIGAILAKINEAGFRITAMKM"
BASE COUNT          895 a          931 c         1084 g         1019 t
ORIGIN      
        1 cctcacccct gacttcgagg tgtatcccaa cccgacgggc ggcgatctga atgtagattt
       61 gaccaactat gtgggcagag cggtgagctt agaggtgtat agcttggaag gcaagttgtt
      121 gcagttcaac aagatagatt aggtgcaaac gactgtcgtg cagatgaata tgagtgattt
      181 gacaacgggc atgtacctga taaaagtcaa atcggccggc ttgccggatg cnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnntacgtca cgcattcttc cgaggaagaa gcccgtcgca
      421 tagccaacgc agcagtatct gcccgcctgg ctgcctgcgc caatttgttt cccattcaaa
      481 gctcttattg gtgggatggc aaaatagagt ctggagctga atgggtgagt gttttgaaaa
      541 ctgcgccgta tttagaacaa cgaatggagg agtttataga acggcttcat ccctacgaga
      601 cgccctgcat cctgcgatgg gaagtaaggg ccaacgaggc ttatgaaaac tggattttgg
      661 cttctgtact aaggaaagaa gaagtggacg gagcaaaggt tgaccgtttt tgatccgttt
      721 ttgctccgcc acttctggtc tttattcatc aatcgtcttc cggtaccggt tctccgtggt
      781 acatgaggaa ggctttgaga aaatcgtcta gatcgccgtc caaaacggca tctgtctggc
      841 tggtctgatg cccggttcgg tgatctttca cgcggcggtc gtcgaggacg tagctgcgga
      901 tttgagatcc ccactcgttt ttcatcttgc cggactcaat cttgtcgcgg gcctctaagc
      961 gctttttcaa ttccagttcg tacaatctgg atttgagcat ttgcatggcc ttgtcccggt
     1021 tctgatgttg cgagcgttcc tgctgacatt ccaccaccaa tcccgaagga aggtgccgca
     1081 cgcgcacggc cgattccgtc ttgttgacgt gctgtccgcc tgcaccgctg gagcggaaag
     1141 tatcccattc caaatcggca ggattgacct ccacttcgat gcgctcgtcc accatggggt
     1201 gaacaaaaac actggagaag gaagtcatgc gcttgccttg tgcattgtac ggactgacgc
     1261 gcaccaagcg atgcacgccg ttttcccctt tgaggaggcc gtaggcaaag tcgccggaaa
     1321 tttccaattc cgcagatcgg atgcccgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnncccac atctggtaca tccggtacag catctcggcc
     1441 cagtcgcagg cttcggtacc gccggcgccc gcattgattt cgagcagaca gttaaattcg
     1501 tcttccggct ggttgagggt ggatcgaaat tcggtatcgt ccactacggc cagcgcttcg
     1561 gcgtatttgg catctacttc ttcttcagtg gctgcgcctt cgcggaaaaa gtcgagcagc
     1621 agctcggcgt cgtccaccac ggtttccacg tctttgtact gtgacaccca gtttttatag
     1681 cttttcagcg ttttcagggt gctttccgct ttttttggat cactccaaaa gtcgggattc
     1741 aaggtctgtt gttcaaggtc ttctatcttg ataagtcgtt catcgacgtc aaagatacct
     1801 cctcaaagcg accagcctct cccgaagatc cttcagttgc tcgttcgtca tcgtagtttt
     1861 tgtttaaatg ttatgttgtt acaaatacct tagcttcaag ctgtatgctt caagctgcaa
     1921 gcttggttgt tggttggata gagaaagttt ccatcagggg aaattttatc aaccatttgg
     1981 aacaaacatg cttgcagttc aacgttgtta cttcaccgaa acgacttcga tgtagaacac
     2041 caatttgctg ttggcgggaa tgaccggagg agcgcctgcc tcgccatagc ccaattcaga
     2101 aggaatgtaa agaatggcct gaccgccctg attgagcaag tcaacgcctt cggtccagcc
     2161 gggaatgaga cccggatcgc cgagtttgaa aggcagcggt gtgccacggc tcagcgaggt
     2221 gtcgaacagg gtgccgtcgg gcagtgcgcc ataataattg acctgtacgt ccttacctgg
     2281 ttcggcagcg gcgccgtcgc cttgcttgag gatgacgtat tgcaagccgg attcggtgct
     2341 tttcatgcct tcaggttgtt tgccgtcaga tactgttttg gcaaattcat tgaccaaagc
     2401 tgctacccgg tcttcttcgg ctttgaggtc agacattttt ttgatgtcca caatgacgat
     2461 atcgtagtag agttctttgg cgtcttcaaa acccataggt ttttgggcca ttgtatctac
     2521 ctgatatacc acggtggcgc tgtcgccgat gctgagtacg cggactaatt cgagtacagg
     2581 agatgccggt tggccgggaa tttcctctgt aggaatttgc aggcttggca tgtcctcgcc
     2641 gctggagcgg gtatcatagt atatggagtc ggagccgtct cttcttacag tggcgtgaaa
     2701 gaagacgata tcgccgggcg cgggcacgtc gccgtctttg tccacatgaa cggtgtagcg
     2761 gaacccggag gcagtaactt gctctttagg tttgttgcat cccgatgcga ggaagagcag
     2821 gccgagtgcc gcgaagagga aaattctcat aaagtgttga ttgataaagt gatgaaatga
     2881 aactgctatc agtagttatt gagcgcgtct ttatactccg ggagcagttc tttgaatttg
     2941 cgaagtgtaa ccttgagcgg ctgaaaggaa aaaccgccgg aggcattgcg gtggccgccg
     3001 cctttgaagt gtttgcggca gatgtcctgc acggaaaaat cacctttgga gcggagggaa
     3061 atcttgacga tgctgggctg ctcggtgatg aacgcggcca tggtgatgtc gcgcattttg
     3121 agcaggtagt tgacgatgcc ttcggtttcg ccgcgcacaa tatcgaagtg ttcgtagtct
     3181 tctttggtga gcgtgatgat gccggtgcgg tattcgggca agatttccat gcggttgtag
     3241 aggcagtgac ccaacaggcg caattgcttc tcgtccaagc tgttgaagat caggtcttgt
     3301 agtttgtaat cgtccacgcc gagttcgagc agttcggcta cgatgcgaaa cagcttgggc
     3361 gaagtgctga atttgaaaga accggtgtcg gtcacaatgc cggtgtaaac gcagtcggca
     3421 gtggcgcggg agatatccgg cctgtgcccc atgaggacga tgaaatcgaa caccatttcg
     3481 gcggtagagc tggccgtcgt gtcggacatg acatagtcgg caaagggttc ggggtagagg
     3541 tggtggtcaa tcagcacttt gaccgagttg agcggctgta tgatttctcc tactttgtca
     3601 atgcggtcga gcgagttgaa gtcgagacaa aaaataattt gtgcccgttc gcatacttcg
     3661 cgggttcgct cctgatcgct gtcgaaaacg atgatgtttt gaataccggg catccagagg
     3721 aacgcatcgg gatattcaga aggcaaaacg acatgcaccg cgtggccctg gctaagcagg
     3781 tagtggtaaa gtcctaaaga tgagcctatt gcatctccat cgggattgcg gtgggaaatg
     3841 atgacaatat ctttgggcga tgccaattgg gcttttaact cgtgtatatt ctccatggct
     3901 gaacgaaatc gggggcgaat ttctcaaaaa aacgagattt caccaaaata ttaaatccgc
     3961 agtgttgcca aatattatgt attgagtagc tttgcgccgc atttaaaaac aaaatatcct
     4021 atggcaggca acaagacctt cacgatgatt aagcccgatg ctgtagaaag cgggcatatt
     4081 ggcgctattc tggccaagat caacgaagcc ggattccgca tcacggctat gaaaatg
//
LOCUS       DAORLF010000429         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11787_length_4124_cov_2.076186, whole genome shotgun sequence.
ACCESSION   DAORLF010000429 DAORLF010000000
VERSION     DAORLF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11787_length_4124_cov_2.076186"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>625)
                     /locus_tag="PK198_23205"
     CDS             complement(<1..>625)
                     /locus_tag="PK198_23205"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF41729.1"
                     /translation="LESNATAGPYTTGGIGNNEGPGGGEFYYGDETGGFGHHELSQGG
                     LGILRGSGETAVSIYDPEQFDSFGVGWYDNENGQENRGYELFYTGNSGGTPGSGTFSK
                     AASLGDLELGVPESPLEIGNRVWEDTNDDGVQDASEPGISGVAVQLWKETTPGNYTQV
                     ATVTTNANGNYYFSNATGTSVLGITYDPDLLPNMNYQLRFPLTNSTLT"
     assembly_gap    626..726
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<727..2332)
                     /locus_tag="PK198_23210"
     CDS             complement(<727..2332)
                     /locus_tag="PK198_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41730.1"
                     /translation="MFSFDHPLKTIISLIFLLDRLIGESFFCIAGTVGIDLSFIRNNK
                     SFLMKKRVCTSYHRMLMIVGMSIFTAFHVVGQVTGTVFRDYNGDGVKQGTEPLVAGVV
                     VNAYLANSAVPCGTTTTSGAAAPNYTLTGCGSSAVRVEFVIPSSGACSLDSDFDFTST
                     GGNSYGSSVQFATGTSSNVNFAINTPADYTGNVSANVPSVYVTCFVNGDPLTTSAGTG
                     DALVGIPYTASGTTPAATHIATAAKIGSAWGLAYSPQAKRIFTSAVLRRHVGLGPQGS
                     GAIYMIDPSGVDLVTNLINLDALLFPTRGSGAYAPSVAGNVVTFTDVIGTNAERGIMG
                     ISQPSRDAAAFDQVGKVGLGDIDLSADGRYLYVTNLYDRKLYEIDLQNAASPVAPSPA
                     NVRFWDIPNPGCTGGTYRPWAVRYYRGKVYVGLVCDAQNSQVAANMSASIYEFDPSGA
                     GSFAGSALLSFPLNYPKGIASDFITSAPTGWFPWTGDFGAFNPETPYKAHPQPIFSDI
                     EIDDDGSFVLGFADRGAMQLGWFNYGP"
     gene            complement(2540..>2809)
                     /locus_tag="PK198_23215"
     CDS             complement(2540..>2809)
                     /locus_tag="PK198_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41731.1"
                     /translation="KKNKGNAFIYVGLPRTTRLKYLPIHVVMKCGVYKQQPLVLVHSI
                     KQAIGFFIKRIHFYVVNFFTQMGEFQDGLLVQSHRPPCSSYLSAV"
     assembly_gap    2810..2828
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2829..3508
                     /locus_tag="PK198_23220"
     CDS             <2829..3508
                     /locus_tag="PK198_23220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF41732.1"
                     /translation="VEFLHTMLTNSIKCNFVIIDYSEELRREVSDFFADSDCMDCVMA
                     VESVEKFLKYHRDFMEIRLVLLAVMLGNQSSIYKIPHIRQRLPNAEIIMFTSIDDTNT
                     IFQALTYGATGYLLKGISMSDLRKSMYDVCEGGGAALNPAVAKKIIQHFVIKDKSIAV
                     ETEEEDKLGEKENIVIHLLKDGLHYQEIAQRMGLSVNGVRYYIKSIYRKLHVKSRAEL
                     VRKHFMK"
BASE COUNT         1115 a          973 c          916 g         1000 t
ORIGIN      
        1 agtcaatgtg ctgttcgtta gggggaagcg caactgatag ttcatattgg gtagcagatc
       61 agggtcataa gtaataccca gtactgaagt ccccgtggca ttgctgaagt aatagttgcc
      121 gttggcgttg gttgtcacgg tagctacctg agtgtagttg cccggcgtcg tttctttcca
      181 caactgcact gctacgccgc tgatgcccgg ctcgcttgca tcctgcacgc cgtcgtcgtt
      241 ggtatcttcc catacccggt tgccgatttc cagaggagat tcagggactc ccaactcaag
      301 gtcccccaga gaggctgctt tggagaaggt gccactccca ggtgtgccgc cgctgttgcc
      361 ggtatagaac aattcatagc cacgattctc ctggccattc tcattgtcat accatccgac
      421 accaaaggag tcgaactgtt caggatcata aatagaaaca gcggtctcgc cggagccgcg
      481 caaaataccc aagccaccct gagaaagctc gtggtgacca aaaccgcctg tttcatctcc
      541 ataataaaac tcaccgccac cagggccttc attgttaccg atgccgcccg tcgtatatgg
      601 gccggcggta gcgttcgact caagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnntcgg gccatagttg aaccagccca actgcattgc accacgatca gcgaacccta
      781 atacaaagga gccgtcgtcg tcaatttcga tatctgagaa gataggctgt ggatgtgctt
      841 tatatggcgt ttcaggattg aaggctccga aatcacctgt ccacgggaac cagccagtag
      901 gtgcagatgt aataaaatcg gaagcaattc ctttaggata attcaacgga aaagacaaca
      961 aagcactgcc tgcaaaactg cccgcacctg aaggatcaaa ctcataaata gaggccgaca
     1021 tattggctgc tacttgggag ttttgcgcat cacacacaag ccctacatat accttgccgc
     1081 ggtagtaacg aaccgcccac ggacggtaag tcccccccgt acaacccgga tttggaatat
     1141 cccagaagcg tacgttagcc ggtgatggcg ctaccgggct ggctgcattt tgaaggtcaa
     1201 tttcatacaa tttgcggtca tataagttgg tcacatacaa gtaacgaccg tctgcagaaa
     1261 ggtcaatatc accgagacca actttaccca cttgatcaaa tgcagcagcg tcgcgagaag
     1321 gttggctaat gcccataatt ccgcgctctg cattcgtacc gattacatct gtaaatgtta
     1381 caacattacc cgctacactt ggtgcataag cgccggaacc acgggtcgga aaaagcaaag
     1441 catccaaatt gatcaggttg gtcaccaaat caacgccgga agggtcaatc atgtaaatgg
     1501 cgcccgaccc ctgaggaccg aggcctacgt ggcggcgcaa cactgctgat gtaaaaatac
     1561 gttttgcctg cggagagtag gccaaacccc acgccgaacc gatctttgct gccgtcgcga
     1621 tatgcgtcgc tgccggtgtc gtacccgaag ccgtgtaagg tattcctaca agagcatctc
     1681 ctgtaccggc agaagtcgtc agcggatcac cattgacaaa acaggtgaca tacacggaag
     1741 gcacattggc cgaaacatta ccggtgtaat cggctggggt attgatcgca aaattcacgt
     1801 tggacgaagt acccgtcgcg aactgtaccg acgaaccgta cgaattccct ccagtactcg
     1861 tgaagtcaaa gtccgagtcg agcgaacaag ctccagaact cggtatcacg aactccaccc
     1921 gtaccgcgct gctgccgcag ccggtgagcg tatagttcgg cgccgcagcc ccggaggtgg
     1981 tcgtcgttcc gcatggaaca gcagaattcg caaggtaagc atttactact acgcctgcca
     2041 ccaaaggctc tgtaccttgc ttcacgccat ctccgttata gtcccggaac acagtacctg
     2101 taacctggcc cacgacgtgg aaagcagtga aaattgacat gccgacgatc atcagcattc
     2161 tgtggtagct tgtacacact ctttttttca tgagaaatga tttgttatta cgtataaaag
     2221 agagatcaat tccgacagtc ccggcgatac aaaaaaatga ctcgccaatg agccggtcaa
     2281 gcagaaaaat aagacttata atggttttga ggggatggtc aaatgagaac aagccaaaca
     2341 ttttttgctc tcacggggca tcttgaagcc ccatatttga cggcaatact tgaggacagt
     2401 gttttttttc atgatgtctt tatgatttgt tatcaggagt aaaccggcgc caataagaaa
     2461 cacatgttct tttcaaagaa aatgcgttta cgcaatcttt tgccgtgaca tattcaacga
     2521 tgtgcgtctt aatgtctgtt tacaccgcgc taaggtagct ggaacagggg ggtctatgtg
     2581 attgaaccaa taagccatcc tgaaattcac ccatttgggt gaaaaaattt actacataaa
     2641 aatgtattct ttttataaaa aatcctatcg cctgttttat actatgaacc aacacgagtg
     2701 gttgctgttt gtaaaccccg cacttcatta ctacatggat gggtaaatat ttcaaccgtg
     2761 tggttcttgg aagacctaca taaataaaag cattaccttt attttttttn nnnnnnnnnn
     2821 nnnnnnnnca gtcgaattcc tccatacaat gcttacaaac tctatcaaat gcaactttgt
     2881 gattattgat tacagcgaag agttgcgccg tgaagtatcc gacttttttg cagattcaga
     2941 ttgcatggac tgtgttatgg cggttgagtc tgtggaaaaa tttttgaaat accatcgtga
     3001 tttcatggaa atcaggctgg ttcttttggc tgtcatgttg ggcaatcaat ccagtatata
     3061 caagattccg cacattcgcc agaggcttcc caatgcggaa attataatgt tcaccagcat
     3121 agacgacacc aacacaattt ttcaggcgct tacttatggc gctaccggct atttactcaa
     3181 aggtatctcc atgtcggatt tgcgcaagtc aatgtacgat gtatgcgaag gaggaggcgc
     3241 cgcgcttaac cccgctgttg ccaaaaaaat catccagcat tttgtaataa aagataagag
     3301 tatagctgtc gaaacagagg aagaagacaa gctcggcgaa aaggaaaata tagtcattca
     3361 cctgctcaaa gacgggcttc attatcaaga aatcgcgcag cgcatgggcc tgagtgttaa
     3421 cggcgtgcga tattatatta aatccattta tcgcaaattg catgtcaaaa gccgggccga
     3481 attggtgcga aagcatttca tgaagtgatg ccgtgcaagc gctctggaga agttatactg
     3541 atccaaacat attagtcccg agaaaaatgc aggcactcaa tatataagct gtgccggata
     3601 cttttttgcg tcgaaggcat ttcacgcctt gacgatgtct tatcaacaat aacgtcccca
     3661 cctcttcagc ccatcgcttt accatcactg aagtagttct gtttcgtata ccacgcatgg
     3721 ctaccgaaat aaccgcagcc tccggtgacg aggatttggg gcatggttgt tttgcgttgt
     3781 tgacgaggca aagttggaga gaaaatcaaa gtccagtgcc atatatacaa aaaaagcggt
     3841 cgctctttga gcaaccgcct cgacaaatca taatcacacg aaaaaatctt aaaggcgcta
     3901 cggcctgtat tttaggggtt tgcgctgcaa atatatcact tttttttaat gcgtaaaaat
     3961 tgttttgaaa aagggggaga accctccctt gcgggaagcc tccccccgat cacataaaac
     4021 aaatttacac atttttaagt gctcttccgg ctcacgaatc ttagtagttg ggcaaaaata
     4081 aatgctcatt ttctttaggc caaaggatta gataagacac ttaa
//
LOCUS       DAORLF010000430         4121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11800_length_4121_cov_2.159124, whole genome shotgun sequence.
ACCESSION   DAORLF010000430 DAORLF010000000
VERSION     DAORLF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11800_length_4121_cov_2.159124"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..488)
                     /locus_tag="PK198_23225"
     CDS             complement(78..488)
                     /locus_tag="PK198_23225"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF41733.1"
                     /translation="MRRTTFALVILLLVGALLWACENRRSYRQGESLYKTHCASCHID
                     DGLGLRGVIPPLAGSDYLARDPTRTACIIRNGLQGEIIVNGITYKEPMAAIPQLSDFQ
                     IANIINYINSAWGNELGYVSVPEIRKRLEECGGR"
     gene            555..1289
                     /gene="bshB1"
                     /locus_tag="PK198_23230"
     CDS             555..1289
                     /gene="bshB1"
                     /locus_tag="PK198_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRF41734.1"
                     /translation="MKIDILAIGVHPDDVELSASGTLLRHIDQGKTAGLLDLTRGELG
                     TRGNADIRDREAAESARLMGAAFRKNICLQDGFFSYTQEALLQIIRVIRQHQPEIVLA
                     NALDDRHPDHGRAAKLTADACFYSGLAKIATFDESGAAQERWRPKAVYHYIQDRNLKP
                     DFVVDIAPYIERKIELIQAFRSQFFLSAAEAEYAGELSTPISGADFMEFIYAKGRSYG
                     RDAGYEFAEGFNVARTPGLEDLFSLH"
     gene            1550..>1836
                     /locus_tag="PK198_23235"
     CDS             1550..>1836
                     /locus_tag="PK198_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41735.1"
                     /translation="MPPTTKSCFQTENRLLQEVQITTWPEQVRYAARDIADTRDQKIR
                     VPATGVYRFRFSNASGERLCRIAIQRRPASDDLRLFDTAVRWEERIDTFYM"
     assembly_gap    1837..1991
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1992..2547
                     /locus_tag="PK198_23240"
     CDS             <1992..2547
                     /locus_tag="PK198_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41736.1"
                     /translation="ETKFKLPENRYLPNRQNPASVSEVTSWAYWIGVGDEADRSFQDA
                     NIKAVSKVADAASGLGLMSGGYGALALLAVKGVAMFSNPPKGDNVRFTLWNDSGKQID
                     SGNSIVAYRRMEAPLQGHFTFQLSNDNMVDGINVTIKILAIVQTQTYRDEAYTEYRRV
                     PLPESERQGKMELRTIRIPHPASF"
     gene            2647..3186
                     /locus_tag="PK198_23245"
     CDS             2647..3186
                     /locus_tag="PK198_23245"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF41737.1"
                     /translation="MRQHFSRCHLLLPAFFGLALLLLTGCGEQPASASGDTVQEIRMP
                     KGSSNADLIRNPISADAPADAGQMASINFSQPVFDFGDIDDKNIVSHTFEFTNTGKAP
                     LVIAHVRGNCGCTVPHWPKDPIAPGAGGKIEVRFDPSGKEGPQNKPIVVTANTYPNAT
                     NITIMANVKASGESGSGEQ"
     gene            3284..3637
                     /gene="yajC"
                     /locus_tag="PK198_23250"
     CDS             3284..3637
                     /gene="yajC"
                     /locus_tag="PK198_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRF41738.1"
                     /translation="MFNTFQPVFLQAGGFDTGSLLFFGAMMLVFWFFLLRPQAKRQKE
                     QRTFMESLQKGDDIVTTSGFLGRINKMDGDIVTVELANKLFVRVTKNSISKEMTDAVY
                     AGGKNKPKEEEKESN"
     gene            3678..>4121
                     /locus_tag="PK198_23255"
     CDS             3678..>4121
                     /locus_tag="PK198_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41739.1"
                     /translation="MGIALVFWLLVKLSGTYRSEKTVELYFSLPEGKVFSALPPKDIT
                     ATIEGTGWELLFDFLRSSQVSLSYDLRGVKDRFVLSRGQLRSEILSRLGSGSMSIVEL
                     NYDDIVLLLEEKSSKRVPVGFRHRFSFVPGYQLQRDPEISPDSVTL"
BASE COUNT          953 a         1123 c         1031 g          859 t
ORIGIN      
        1 atgaccgttc aaggttccgg atcccctcgc gctgagcacc gcctaccgag caccgcccac
       61 cgcctaccga gcaccgccta ccgcccacca cactcctcca gtcgcttcct gatctccggc
      121 accgacacat agcccagttc attgccccaa gccgaattga tgtaattgat gatgttggca
      181 atctgaaaat cgctcaattg cggaatggcc gccatgggtt ctttgtaggt aatgccgttg
      241 acgataatct ccccctgcaa tccattccga atgatgcagg ctgtacgcgt cggatcgcgg
      301 gccaaataat cggagccggc caaaggtgga atgacacccc gaagccccag tccgtcgtct
      361 atatggcagg aggcgcagtg ggttttgtac aaactctcgc cctggcgata cgagcgccgg
      421 ttttcgcagg cccacagcaa tgcccccaca aggagcaaaa taaccaatgc aaatgttgtg
      481 cggcgcataa cttacttttg tcggctaaac catttttgca cttcattggt ttgaaaacgc
      541 tcaatctctt caccatgaaa atagacatcc ttgccatagg cgttcacccc gacgatgtgg
      601 aactcagcgc ctccggaacc ctcctgcgtc acattgacca aggcaaaacg gcgggcttgc
      661 tcgacctcac gcgtggcgaa ctgggtaccc gcggcaatgc cgatatacgc gaccgcgaag
      721 ccgccgaatc ggcccgactt atgggcgctg cttttcgcaa aaatatatgc ctgcaagacg
      781 gattcttcag ctacacgcag gaagccctgc tgcaaatcat ccgcgtcatt cggcaacacc
      841 aacccgaaat cgtgctcgcc aatgccttag acgaccgcca tcccgatcat ggccgcgccg
      901 ccaaactcac cgccgatgcc tgtttttact caggtctggc caaaatcgcc accttcgacg
      961 aatcgggagc cgcgcaggaa cgctggcgcc ccaaagccgt ttaccactat attcaggacc
     1021 gaaatctgaa gcctgatttt gtggtggaca tcgcgcccta catcgaacgc aaaatagaac
     1081 tcatccaggc attccgctct cagttcttct tgtctgctgc tgaagcggag tacgccggcg
     1141 agctttccac gcccatttcc ggcgctgatt ttatggagtt catttatgcc aaaggtcgtt
     1201 cgtatggccg ggatgcaggg tatgaatttg ccgagggctt caatgtggcg cgcacgccgg
     1261 ggttagagga tttattctca ctccattaat ggaggcaatg ttggcggaag atacaatatc
     1321 ctcttttttc ccgacgcagc agcctgcaac tttggcgaag gccctgttgt ttttacgggc
     1381 aatatcgcga cataacttta tcaaccaaga acatttatga aatcattcgt ttcggccatt
     1441 gtcgccgcct ttctgtttgc ttcggcaatg caagcccagc agcccctcac ggtggccgat
     1501 atgaccctgc gcttgtcggg taaagaaacc aaagaactgt tttacagctt tgccgccgac
     1561 gacgaaatcc tgttttcaaa ccgaaaatcg gctgcttcag gaagtacaaa tcactacctg
     1621 gcccgaacag gtgcgctatg ccgcccgaga cattgccgat acccgcgatc aaaaaatcag
     1681 ggtgcccgct accggtgtgt atcgctttcg gttcagcaat gcttccggcg aaaggctctg
     1741 tcgcattgcc atacagcgcc gcccggcctc cgatgacctg cgcctgtttg ataccgctgt
     1801 gagatgggag gagcgcattg acacctttta tatgaannnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nggaaaccaa attcaaactg cccgaaaacc gctacctgcc caaccgccaa
     2041 aatcccgcat cggtgagcga agtgacgagc tgggcctact ggatcggcgt cggcgacgaa
     2101 gccgaccgca gttttcagga cgccaacatc aaggcggtga gcaaagtagc cgatgccgct
     2161 tcggggctgg gcctcatgtc gggcggatac ggcgcgctgg cattgctggc agtgaaaggc
     2221 gtggcgatgt tcagcaatcc gccgaaaggc gacaatgtga ggtttaccct ctggaacgac
     2281 agcggcaaac aaatagacag cggcaacagc atcgtggcgt atcgtcgcat ggaggcgcct
     2341 ttgcaggggc attttacctt ccaactgtcc aatgacaata tggtggacgg catcaatgtg
     2401 accatcaaaa tattggccat cgtacaaact cagacgtacc gcgacgaggc atacacggaa
     2461 taccgccgcg tgccgctgcc ggagtcggag cgccagggca aaatggagtt gcgcaccatt
     2521 cgcataccgc acccggcttc tttttgatcg ggaaatttat acctttgtcg cgacacttgc
     2581 ctgacagtaa ggcgacagac cgttgcccgc ccatcaggcg ccgacagatc actaaataga
     2641 ctcgctttga gacagcattt ctcgcgttgc catcttttgt tgccggcttt ctttggcttg
     2701 gctttgctcc ttttgaccgg atgcggagag caaccggcgt ccgcttccgg cgataccgta
     2761 caggaaatcc gcatgcccaa aggcagcagc aatgccgacc tgattcgcaa tccgatcagc
     2821 gccgacgcgc ccgccgatgc aggccaaatg gcgagcatca atttctccca acctgttttt
     2881 gacttcggcg atatagatga caaaaacatc gtaagccaca catttgagtt taccaatacc
     2941 ggtaaagcgc ctttggtcat agcgcatgtg cggggcaact gcggatgtac cgtgccgcac
     3001 tggcccaaag acccgatagc gcccggcgcc ggcggcaaaa tagaagtgcg cttcgatcca
     3061 tcagggaaag aaggtcctca aaacaaacca atcgtcgtga cggccaatac gtacccgaat
     3121 gccacgaata ttaccatcat ggcgaatgtg aaggcgagcg gggagagcgg gagcggggag
     3181 cagtaggcgg tgcgaagctt aggatggcac agcttgcagc ttgcggctaa aagccagcag
     3241 ccgttcctac ccgaaacaaa aacattcatc aatcaatacg ttcatgttca acacattcca
     3301 acccgttttt cttcaggcag gaggtttcga caccggcagc cttttgtttt ttggagctat
     3361 gatgctcgtt ttctggttct tcttgttgcg cccgcaggcc aagcgccaaa aggaacagcg
     3421 cacgtttatg gagagcctgc aaaaaggcga cgacatcgtg actacgagcg gttttctggg
     3481 ccgtatcaac aaaatggacg gcgacattgt gaccgtagaa ttggccaaca aactcttcgt
     3541 gcgggtgacc aaaaactcga tttctaaaga aatgaccgat gccgtctatg ccggaggaaa
     3601 aaacaagcca aaagaggaag agaaggagtc taattgagtt tccgaccgga gaagaccgca
     3661 aaatactcgg catttgcatg ggcattgcgc tcgtgttttg gctcttggta aaactgtcag
     3721 gcacttaccg ttcggaaaaa accgtagaat tgtacttctc attgccggag ggcaaagtgt
     3781 tttcggcctt gcctccaaaa gacatcaccg ccaccataga aggcacggga tgggaattgc
     3841 tctttgactt tttgcggagt tctcaggtgt cgctcagcta cgatttgcgc ggcgtaaaag
     3901 atcggtttgt actcagccgg gggcagttgc gcagcgaaat cctgagccgc ctcggttccg
     3961 gcagcatgag cattgtggaa ctgaactacg acgacattgt gctgctgttg gaggaaaaat
     4021 cgtccaaacg cgtaccggta ggatttcggc atcggttcag ttttgtgccg ggctaccagc
     4081 tacaacgcga tccggaaatc agccccgaca gcgtgacctt g
//
LOCUS       DAORLF010000431         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_11857_length_4108_cov_2.180360, whole genome shotgun sequence.
ACCESSION   DAORLF010000431 DAORLF010000000
VERSION     DAORLF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11857_length_4108_cov_2.180360"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>294
                     /locus_tag="PK198_23260"
     CDS             <1..>294
                     /locus_tag="PK198_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF41740.1"
                     /translation="GESEPIDPVAGHIPGAVNAFFMENLDEGGRFLPAEQLRGRFLPM
                     LDGLPAQQSVFYCGSGVSACHNLLAIAHAGLGDAGLYAGSWSEWIAAPERPVA"
     assembly_gap    295..355
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            567..>1639
                     /locus_tag="PK198_23265"
     CDS             567..>1639
                     /locus_tag="PK198_23265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRF41741.1"
                     /translation="MSYFNEFLQTWRGFFNQWKSRIVAKWADFKTRYPRWALAVKAGA
                     GLGVFGFATLMAIVFMVYMEWLGPLPTYGELKGIRNNTASEVFSEDKVLLGKYYKENR
                     VNASISEISPHIVEALIATEDSRFFRHSGIDLRAWVRVLVRTVLLFDESGGGGSTLSQ
                     QLAKNLFPRRKYRLLGTVINKVREMFIARRLERTYTKEELLNLYLNTVPFSENTFGVK
                     VAAQRFFATTPQKIKIEEAATLIGTLKGNTIYSPVRHPERCLQRRNTVLNQMTKYGYL
                     EQSVCDSLKTLPLELKYFKEGADQGLATYFREHLRLELEQILKDHKKKDGTPYNLYTD
                     GLKIYTTINSRMQEHAETAMRDHL"
     assembly_gap    1640..1678
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1679..>2199
                     /locus_tag="PK198_23270"
     CDS             <1679..>2199
                     /locus_tag="PK198_23270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF41742.1"
                     /translation="LKSWGKKGPWNDPKLLQRAMKQSDRYKALKAEGVSDKEIEKIFN
                     TPAKMNVFDWEDKEELREITPMDSIKLHLALLQAGFLAMEPQTGIIRAWVGGIDFEFF
                     KYDHTKSRRQVGSTFKPIVYASALMNGMLPCEYTENMLTMYEAYDNWEPQNSDEKYGG
                     AYSMEGALANSVN"
     assembly_gap    2200..2215
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2216..3095
                     /locus_tag="PK198_23275"
     CDS             <2216..3095
                     /locus_tag="PK198_23275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF41743.1"
                     /translation="LMRAGVNNVRMLAQDMGVSSDVPRVPSIALGTAEASLREMLTVY
                     ATLANNGRRPLIHYLDRIETADGKVLEKFGRPDPGAFRQVVPAEYAGMVTRMLQTAVN
                     EGTGSRLRSEFGLTGDIAGKTGTTQDNTDGWFIGYTPRLVAGAWVGAELPQVRFRYTS
                     QGQGARSALPIWGRFMRKVVNDGNLQRWRGGAFTPLPDTIAAMMDCPLYLDEMPVDGY
                     MVRDSATLGILLEEMMEGEGPAMKIRSRRDGETEEEYQAYLQKQLEKQRRREEKQESR
                     KDHWSETLFGKKKENQ"
     gene            3507..3836
                     /gene="ssb"
                     /locus_tag="PK198_23280"
     CDS             3507..3836
                     /gene="ssb"
                     /locus_tag="PK198_23280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRF41744.1"
                     /translation="MNNLRNRVQLIGNLGRDVEIKSLDNGRSMARASIATREVYRNDE
                     GEKNIDVQWHNLVAWGKMAEMMHVLCKKGKEVAVQGKLTHRMYEDKDGVTRYTSEIVV
                     SEFMLIN"
BASE COUNT         1012 a         1065 c         1040 g          875 t
ORIGIN      
        1 ggggcgagtc ggagccgatt gacccggtgg cgggccatat tcccggtgcg gtgaatgctt
       61 tttttatgga aaatttggat gagggcgggc gctttttgcc ggctgaacaa ttgcgcgggc
      121 gattcctgcc gatgctggac gggctgcccg cccaacagtc tgtattttat tgtggatcag
      181 gcgtttcggc atgtcataac ctgttggcca tcgctcatgc cggcttgggc gatgcgggtt
      241 tgtatgccgg ttcctggagc gagtggatcg cggccccgga gcggccggtg gcgcnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg
      361 gtttcgcgtt tcgggttcaa ggtttcgggt ttcatctgcc gaagccttag cgaagggggg
      421 gggcgggttc aaagtttcgg gttcaaggtt tcgcgttccc tcgcgattcg caccgcacac
      481 cgcctactgc ctaccgacat accgcatacc gcataccgat ccactttttt atctttgccg
      541 ttcatttttc agactctggt aagcgaatga gttatttcaa tgaatttttg cagacatggc
      601 ggggcttctt caatcaatgg aaaagccgca tcgttgccaa atgggccgat ttcaaaaccc
      661 gttatccgcg ctgggctttg gccgtcaaag caggggccgg gctgggtgta tttggcttcg
      721 ctacgctcat ggccattgtt tttatggtat atatggaatg gctggggcct ttgccgactt
      781 atggcgaact gaagggcatt cggaacaaca ccgcctccga ggttttttcg gaagacaagg
      841 tgctgttggg caagtattac aaagaaaacc gcgtcaacgc gtccatttct gaaatttctc
      901 cccacatcgt ggaggcgctc attgccaccg aagactcccg ctttttccgc cacagcggca
      961 ttgacttgcg cgcttgggtg cgggtgttgg tgcgcaccgt attactgttc gacgaatcgg
     1021 gcggcggtgg aagcacgctc agtcagcaat tggccaagaa cctttttccg cgacgcaagt
     1081 atcgcctgct tggtacggtt attaataaag tgcgcgaaat gttcattgcc cggcgattgg
     1141 aacgcaccta caccaaagaa gaactgctga atttgtacct caataccgtg cctttcagcg
     1201 aaaacacctt tggagtaaaa gtggcggcgc agcgcttttt tgccactacg ccccagaaaa
     1261 tcaagatcga agaagcagcc accctcatcg gcaccctgaa aggcaacacc atttacagcc
     1321 cggtgcgcca cccagaacgc tgcctccaac gtcgcaatac cgttctcaat cagatgacca
     1381 aatacggcta tttggaacag tccgtttgtg actcgctcaa aaccctgccg ctggaactga
     1441 aatattttaa agaaggcgcc gatcagggat tggccaccta ctttcgggag catctgcgat
     1501 tggaattgga gcaaattctg aaagaccaca aaaaaaagga tggaacgccc tacaacctct
     1561 ataccgacgg gctgaaaatt tacaccacca tcaactcccg gatgcaagaa cacgccgaaa
     1621 ccgctatgcg cgaccacctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     1681 tcaaatcctg gggtaaaaaa ggcccctgga acgaccccaa gctcctgcaa cgagccatga
     1741 aacaatcgga tcgctataaa gcgctcaaag cagaaggcgt atcggacaaa gaaattgaga
     1801 agattttcaa tacgcccgcc aaaatgaatg tcttcgattg ggaagacaaa gaagaactcc
     1861 gggagatcac gccgatggat tccatcaaac tgcatcttgc tcttcttcag gctggcttct
     1921 tagctatgga accccaaacc ggcatcatcc gtgcctgggt gggcggcata gactttgaat
     1981 ttttcaaata cgatcacacc aaatcgcggc ggcaggtggg cagtacgttc aagcccatcg
     2041 tgtatgcctc tgcgctgatg aacggcatgt tgccctgcga atacaccgaa aatatgctga
     2101 ccatgtacga agcgtacgac aactgggagc ctcaaaattc ggatgaaaaa tacggcggag
     2161 cttattctat ggagggcgct ttggccaatt ccgtcaacan nnnnnnnnnn nnnnngctca
     2221 tgcgcgcggg cgtcaacaac gtgcgaatgc tggcccagga catgggcgtc agcagcgatg
     2281 ttccgcgcgt gccgtccatc gcattgggca ctgccgaagc ctctctgcgc gaaatgctca
     2341 ctgtatatgc tactttagcg aacaatggcc gccgcccgct gattcactat ctggaccgca
     2401 tcgaaaccgc cgacggcaaa gtgttggaga agttcggaag acccgatccc ggcgcgttcc
     2461 ggcaagtggt gcccgctgaa tacgccggca tggtgacgcg catgttgcaa accgccgtca
     2521 acgaaggcac gggcagccgc ctgcgctcgg aattcggcct caccggcgat attgcaggca
     2581 aaaccggcac tactcaagac aataccgacg gctggttcat cggctacaca ccgcgtttgg
     2641 tggccggagc ctgggtgggc gccgagctgc cgcaggtgcg tttcagatat acctcgcaag
     2701 ggcagggcgc tcgctctgcg ctgccgatat ggggccggtt tatgcgcaaa gtggtcaacg
     2761 acggcaacct gcaaagatgg agaggcggcg cctttacccc actgccagat accattgccg
     2821 ccatgatgga ctgccccttg tacttggatg aaatgcccgt ggacggctac atggtacgcg
     2881 acagcgccac actcggcatc ctgctggaag aaatgatgga aggagaaggg ccggccatga
     2941 aaatcaggtc tcgccgcgat ggagagacgg aagaggaata tcaggcatat ttgcaaaaac
     3001 aactcgaaaa gcagcgccgc agagaagaaa aacaggaatc cagaaaagac cactggagcg
     3061 agactttgtt tgggaagaaa aaggagaatc aatgaaggga cggagcaagt ccgtttgatg
     3121 aggtatcccc ggtgctttag tgcagtgtaa attaaatttc cgtgtttagt aaaggagtga
     3181 ttgcgggctg tggcttggtg cggtcactcc gtcaccaccc cggaaaaaga aaacactcgt
     3241 aaatcacaat gcactgaatg tgattttttt aaaagtaagc cctatttctg atattaaata
     3301 caagtaattg attgtcagtg ttttggaatt taaaacatgt tgtgctattc gattacaaac
     3361 ggctacaaac ggctacaaac gacattaaac gtttattata cgactatcgg aaaccccgcc
     3421 ccacaacttt gcagcgtcgt tcggttcaac caattcccgg cggcagaaag caaccttttt
     3481 cttcaacttt tcaaaaacat taagtcatga acaacttgcg taaccgcgta caactcatcg
     3541 gcaaccttgg ccgcgacgtg gaaattaaat ccttggacaa cggccgctct atggcccgtg
     3601 ccagcatcgc cacccgcgaa gtatatcgca acgatgaggg cgaaaaaaac attgacgtgc
     3661 aatggcacaa cctcgtagcc tggggcaaaa tggccgaaat gatgcacgta ctctgcaaaa
     3721 aaggcaaaga agtagctgtg cagggcaagc tcacccaccg catgtacgaa gacaaagacg
     3781 gcgtgacccg ctacacttcc gaaatcgtcg tcagcgaatt tatgctcatc aattaatcac
     3841 agcttgcagt agctgcatgc tgttcgatac gatctccgta tgaaaccgcc tgctgcctac
     3901 cgcacaccgc ctactttttt ccttcactcc attaaaatct tttcaatcat gtccaccatt
     3961 agaaacagtg taacgctcat cggccacttg ggccgcgacc ccgaaatccg caactttgaa
     4021 agcggcaaat cgaaggcttc tttttccttg gccaccaacg aaacctaccg cacggcaaaa
     4081 ggcgaatcgg taaccaccac gcaatggc
//
LOCUS       DAORLF010000432         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12007_length_4068_cov_2.391478, whole genome shotgun sequence.
ACCESSION   DAORLF010000432 DAORLF010000000
VERSION     DAORLF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12007_length_4068_cov_2.391478"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>1161)
                     /locus_tag="PK198_23285"
     CDS             complement(93..>1161)
                     /locus_tag="PK198_23285"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF41745.1"
                     /translation="AATPPAAPPALQIGVETNMIFRAVGGNTALSISNPQEFYQIALS
                     GQLYNLSNLGPEPPDRELAFVRTVSNNAFRYSESIRDAYNSASNQADYPDTGLAERLA
                     IVARLIKGKLGSKVYMVTIDGFDTHADQALNHPRLLASVANSVAAFWQDLGAGGQSQN
                     VLAMTFSEFGRTIYENGSLGTDHGTGAPMLVFGDGIGNGFHGTAPDLVNTDQYGDPFY
                     SVDFRDAYSTVLQNWLCLHPDVIQTVLQRPLGNGIAGLLPPSSPPPPLNNPAALLGHH
                     PAESEPGAISIKYSIQSRGQVRLSIMLPNGSALRTLVDEFQERGSYTFHFRPSRFFLA
                     PGEYVYRLETGGRIYQRNLKV"
     assembly_gap    1162..1194
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1195..1761)
                     /locus_tag="PK198_23290"
     CDS             complement(<1195..1761)
                     /locus_tag="PK198_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRF41746.1"
                     /translation="MHDHNMPSGKHPKAAQHLRRRYGASMEQQTEHEQAHRAFGRRDF
                     LKLTGLAAAGGSMMLGSNTLRAFAPGALLSQLALSDCGDRTLVLIRLKGGNDGLNTVI
                     LRGNDEYYNIRPTLAVQEPDLWALSQDFGMPNYMQALQPLWEEGKMRVIHNVGYPDAN
                     YSHFRSSDIWASASDSNELVATGWIGRWL"
     gene            complement(1894..>2107)
                     /locus_tag="PK198_23295"
     CDS             complement(1894..>2107)
                     /locus_tag="PK198_23295"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41747.1"
                     /translation="ARRVTAELIGHYLKRELEPALFETALAYFKGEVPENYFEDGTWS
                     VQYVETPYQIINLLYYLTRLPEWQLC"
     assembly_gap    2108..2174
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2175..3357)
                     /locus_tag="PK198_23300"
     CDS             complement(<2175..3357)
                     /locus_tag="PK198_23300"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF41748.1"
                     /translation="MHTSATLLPPLDPFVPNADQPWDARRVQHFYQRIGLAASYEQVV
                     QGLAMSPSELADSVIDALLAAPDPVAPIWANWTWDDYQDNNDLYFEHKYEFRLRWLRE
                     MIEEGLRSKLALFWHNHFVTEEVVYDCNSFMWAYYLLLHKNALGNFRTFVEEMGKNPA
                     MLVYLNGNINVAGQPNENYARELMELFTMGEGNGYTQTDIVEVARALTGWRVDRYNCD
                     NAVTFAPNRHDNGIKTIFGQAGNWGYNDVHELIFTLRRDETARHICGKIYRAFVYQKI
                     DEQVVEAMAETFKANNWELAPVFRLLFKSTHFYEDRHINTCIKSPIETMTGLFRLSGV
                     TSPDEINDDNIGYLNYIVSEMGQEPFNPVDVAGWPGYHAWLNENTLAFRWSFSASFLF
                     GL"
     gene            3442..>4068
                     /locus_tag="PK198_23305"
     CDS             3442..>4068
                     /locus_tag="PK198_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41749.1"
                     /translation="MKAIYLSCFLMASALLSVQAQTVLFSEDFNSCSLSDGWTVHLNG
                     NPDADWYVGMPQNNSSDGSTIDGSCMLIIDDDATGENTPPWNLELISPAFDITGWTTA
                     TVSMDVHFRNFDGMDSLTIYAYDGQNYHLLGKYRGGASQTGTQFSQFVPLIADLSFFP
                     SSTMQLVIRYDDGGTWAWWAGIDNIVVRGEGTARNFVLEQFNDCALPEG"
BASE COUNT          831 a         1058 c         1185 g          894 t
ORIGIN      
        1 cgctaccccg cgttcctcct tacgtcggac gcggggctat tcaaattgaa ctgccgtcgg
       61 cagttccccc ctcgcggcac gagttgcccg cattacacct tcagattcct ttgataaatg
      121 cgccccccgg tttcgaggcg atacacatac tcgcccggcg cgaggaaaaa gcgcgagggc
      181 ctgaaatgga acgtataact accccgctcc tgaaactcat ctaccagcgt gcgcaaggcc
      241 gagccattgg gcaacataat ggaaagccgc acctgtcccc ggctctgaat gctgtatttg
      301 atggaaatgg cgcccggctc cgattcagcc ggatgatgcc ccagcaaggc tgccggattg
      361 ttgagcggcg gcgggggcga agaaggcggc agcagtccgg cgatgccgtt gccgaggggg
      421 cgttgcagca ccgtttgtat cacatcggga tgcaggcaga gccagttttg gagcaccgtg
      481 ctgtacgcgt cgcggaagtc aacggagtag aacggatcgc cgtattggtc ggtattcacc
      541 aagtcggggg ctgtgccgtg gaatccgttg ccgatcccgt cgccgaagac cagcataggc
      601 gcgccggtgc cgtgatcggt gccgaggctg ccgttttcgt aaatcgtgcg accaaattcc
      661 gaaaaagtca tggccaatac gttttgcgac tgcccgccgg cgccgaggtc ttgccagaac
      721 gccgccacgc tgttggccac agaagccagc agccgggggt gattcagcgc ctgatcggcg
      781 tgggtatcga agccgtcaat ggtgaccata tacactttgc tgccgagttt gcctttgatg
      841 aggcgcgcca cgatggccaa tcgttcggcc aagccggtgt cggggtaatc ggcctgattg
      901 gaggcggaat tgtaggcgtc gcggatggac tccgagtagc ggaaggcgtt gttggacacc
      961 gttcgcacga aggccaattc gcggtcgggc ggctccggtc cgaggttcga caagttgtac
     1021 aactgcccgg aaagcgctat ctgataaaat tcctgcggat tgctgatgct cagggctgta
     1081 ttgccgccca cggctctgaa aatcatattg gtttccaccc cgatctgcaa ggcgggcggc
     1141 gcggcgggcg gtgtggcagc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaacca
     1201 gcgcccgatc cagccggtgg cgaccaattc gttggaatcg gaagccgaag cccagatgtc
     1261 gctcgatctg aaatgcgaat aattggcatc ggggtagccc acgttgtgaa tgacgcgcat
     1321 tttgccttct tcccacagtg gctgcagggc ctgcatgtag ttgggcatac cgaaatcctg
     1381 gctcaaggcc cacaaatcgg gttcctgcac ggccagagtg gggcggatgt tgtagtactc
     1441 gtcgttgccc cgcaggatga cggtattgag gccgtcgttg ccgcctttga gtcgaataag
     1501 cacgagggtg cggtcgccgc aatcgctgag tgccaattga gagagcaggg cgcccggcgc
     1561 aaaggcgcgc agggtattgc tgcccagcat catggagcct ccggcggcgg cgaggccggt
     1621 gagtttgagg aagtcgcgac gaccaaaggc gcggtgtgcc tgttcgtgtt cggtctgctg
     1681 ttccatagat gcgccatagc gacgacgcag gtgttgtgct gcttttgggt gtttgccgga
     1741 aggcatattg tgatcgtgca tggttatggt agcggttaaa tgaagtagtt aaatagctgc
     1801 gagcttcgag ttgtctggct tagtgaaggg gtgactactg ggagatggac ttgacggagt
     1861 caccccttca ctaaacatgt aaaaaagctt ccttcaacaa agctgccatt ccggcaaacg
     1921 cgtgagataa tacagcaaat tgatgatctg gtaaggcgtc tccacatact gcacgctcca
     1981 ggtgccgtct tcgaagtagt tttctggcac ctcgcctttg aagtaggcca gcgccgtttc
     2041 aaaaagcgcc ggttcgagtt cccgtttcag gtagtgcccg atcagttcgg cggtgacgcg
     2101 gcgggcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnacaaac cgaagagaaa cgaagcgctg aaactccacc tgaaagcgag
     2221 ggtattttcg ttgagccagg cgtggtatcc cggccaaccg gctacgtcca ccggattgaa
     2281 cggctcctgc cccatttcgg agacgatgta attgaggtag ccgatgttgt cgtcgttgat
     2341 ctcatcggga gaggtcacgc cgctgaggcg gaacagccct gtcatggttt caatcggact
     2401 tttgatgcag gtattgatgt ggcggtcttc gtaaaaatga gtgcttttga acagtagtcg
     2461 gaacaccggc gccagttccc agttgttggc tttgaaggtt tcggccattg cttccaccac
     2521 ctgttcgtct attttttgat acacaaaagc ccggtatatt ttgccgcaga tgtggcgggc
     2581 agtttcgtct cggcggaggg taaaaatgag ttcgtgaacg tcgttatacc cccaattgcc
     2641 ggcttggccg aagatggttt ttatgccgtt gtcgtggcgg ttgggagcaa aagtgacggc
     2701 attgtcgcaa ttgtacctgt ccacgcgcca gccggtgagt gcgcgggcca cctccacgat
     2761 gtcggtttgg gtgtagccgt tgccctcgcc catggtgaag agttccatga gttcgcgggc
     2821 gtagttttca ttgggttggc ctgccacgtt gatgttgccg ttgagataga ccagcatggc
     2881 ggggtttttg cccatttctt ccacgaaagt gcggaaattg cccagcgcat ttttgtgcag
     2941 cagcaggtag taggcccaca tgaaggagtt gcaatcatac actacctctt cggtgacgaa
     3001 gtgattgtgc caaaagagtg ccagtttgga gcgcagccct tcctcgatca tctcgcgcag
     3061 ccatcgcagg cgaaactcgt atttgtgttc aaaatacaag tcgttgttgt cttggtaatc
     3121 atcccaggtc cagttggccc aaatcggcgc tacgggatcg ggggcggcca gcagggcgtc
     3181 tatcacgctg tcggccaatt cggaaggcga catggccaaa ccctgcacca cttgttcgta
     3241 agaggcggcc aggcctatgc gctgataaaa atgttgaact ctgcgcgcat cccagggctg
     3301 atctgcattg ggcacaaaag gatcgagcgg cggcaggagg gtggcggatg tatgcatgct
     3361 ttgatacaat gaaatttaaa aaaatccctc aataatagct caaatcggga agtgcgtgtt
     3421 acttttattg caaaaaaaat cgtgaaggca atctacttat cctgcttcct catggcttcg
     3481 gcgctgttaa gcgttcaggc tcaaacagtt ttgttttctg aagacttcaa ctcctgctcg
     3541 ctctccgacg ggtggaccgt acatctcaac ggcaatcccg atgcagactg gtatgtggga
     3601 atgcctcaaa acaacagcag cgatggctct accatagacg gctcctgcat gctcatcatt
     3661 gacgacgacg ctaccggaga gaacacgccg ccctggaatc tggagttgat ttcgcccgcg
     3721 ttcgacatca ccggctggac gaccgccacc gtgtctatgg atgtacattt ccgcaatttt
     3781 gatggcatgg acagcctcac gatttatgcc tacgacggcc aaaactacca cctgctcggc
     3841 aaataccggg gcggggccag ccaaacaggc acgcagtttt cgcagtttgt gccgctcatc
     3901 gcagacctct ccttttttcc ttcttccacc atgcaattag tcatccgcta cgacgacggc
     3961 ggcacctggg cctggtgggc gggtatagac aacatcgtgg tgcgcggcga aggcacggcc
     4021 cgcaatttcg tactcgaaca gttcaacgat tgcgcgctgc ccgaaggc
//
LOCUS       DAORLF010000433         4033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12152_length_4033_cov_1.837607, whole genome shotgun sequence.
ACCESSION   DAORLF010000433 DAORLF010000000
VERSION     DAORLF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12152_length_4033_cov_1.837607"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>799
                     /locus_tag="PK198_23310"
     CDS             <1..>799
                     /locus_tag="PK198_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRF41750.1"
                     /translation="VLFLRSVYEIVTHTGAGFLDSLAGLVFFLLLGKWFQQKTYHNIS
                     FERDYKSYFPIAATKKENGEEVTISLDKLQPGDIIVIRHKELIPADAVLLNGEGSIDY
                     SFVTGESEPVLRQSGEKIYAGGRQMGGAIEISLTRKVSQSYLTQLWNDEAFTKKIESN
                     TSRIANRIGKYFTTAILLIGLGTLIYWLPKDGNIAFNAFTAVLIVACPCAVALAVPFI
                     FGNVLRILARNKFYLKNTSVIESIEEADAVVFDKTGTITQRQNSEIWF"
     assembly_gap    800..821
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <822..1644
                     /locus_tag="PK198_23315"
     CDS             <822..1644
                     /locus_tag="PK198_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRF41751.1"
                     /translation="IGLGGDARGLAEHGVAQGQHGQPVDLADHRAEPLNWHEATGQGV
                     EAVFGQRTLRLGSKDFIGRDIQEVLHSDDAGVFLQINDSLRGYFRIDQRFRPGTQEAL
                     QFFSQQAPIYLLSGDNDREKESVLPLFGSEDRLRFFQSPQDKLQFVKGLQNEGRKVMM
                     LGDGLNDAGALQQSNAGIVLAEDTNNFTPACDAILHSAQFRRLPDFVQLVRRSTKLVY
                     YAYGLAFVYNVIGLSYAVQGLLSPVVAAILMPTSSITIVVFGMLSSNWLGRRLRL"
     gene            complement(1747..>2433)
                     /locus_tag="PK198_23320"
     CDS             complement(1747..>2433)
                     /locus_tag="PK198_23320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41752.1"
                     /translation="GADMQTFTQGVAGFDMDYALALAYDADYQRLWVTGNVEHAQYFD
                     GSIWHTESFPLPAAGHQWIMRVHARDGFAAMSSSERVLLYAEGQWQEFNPTNSPLSGG
                     AMHGLGLDAHNRLWISHWQPWALDIYETGLISSAVKPESSNGAIEMRVFPNPTAERAG
                     LEISCREASTSAALRITDVDGRLVSTTSHSLQSGHNYLEVNVSRLSPGIYFMLLDTDS
                     ERFYGRMVKH"
     assembly_gap    2434..2443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2444..3934)
                     /locus_tag="PK198_23325"
     CDS             complement(<2444..3934)
                     /locus_tag="PK198_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41753.1"
                     /translation="MNRMMSFLLLLAATLTNISLLAQAGTWHTYPIPSKVNDWMEWEG
                     QLWAATDAGIFIINPSTGQVAEHLTKANAGLPSNSVEALTIDPQTLQPFIGTYDVALA
                     FRSPDGQWQSIDYPAFIRNQAGGNMLLTYCMEFDNEGRLWAGTSVGLARYTDGEWTLF
                     NQSAGLSFLGAVWSMAKTEDGAILAASNVMYKVPQGEETPQLFSPSPSGNNGFFLFAY
                     GDAKAFTASNGDQWFFTDIGRAGRFHNGAWELYDPAQSVGVGHRKPQIINELPDGSLS
                     IYYSDNLRFLRFHNGEWTLESTYTGALPIIGAYRSEDFDIILNSKEILIPGAQDTAMA
                     ALLNYPFESLPERFQTDHQKQLWTVTEDRRLLNLDSGAAIPCIFNGQSFAVGNFSFAP
                     DGSIWATSGFHLYHYQLGQWTRYDHTNSLLPAQKLLTRITVDDLGRVWVYVYQDAIYR
                     FDGSNWMRYPHPLFSNYYVYAMTGGANGDLWFSSYLNGQSLTVGRFN"
BASE COUNT          986 a         1046 c         1082 g          887 t
ORIGIN      
        1 gtgctgtttt tgcgcagcgt ttatgaaatc gtgacgcata ccggcgccgg attcttggac
       61 agtctggcag gcctcgtgtt cttcctgctg ttgggcaagt ggttccagca aaaaacctat
      121 cacaacatct ctttcgagag agattacaaa tcctatttcc ccattgccgc caccaaaaag
      181 gaaaacggcg aggaagtaac catttctttg gacaagctcc aacccggcga catcatcgtc
      241 attcgccaca aagagctgat ccccgccgat gctgtgctgc tgaatggcga aggcagtatt
      301 gattacagtt tcgtgaccgg cgaatcggag ccggtgctac gccaaagcgg cgaaaaaatc
      361 tacgccggcg ggcgtcaaat gggcggcgcc atcgaaatca gcctcacgcg caaggtctcc
      421 caaagctacc tcactcaact gtggaacgac gaggcattta ccaaaaaaat cgaatcgaat
      481 accagccgca ttgccaaccg aatcggcaag tatttcacca cggccatcct gctcatcggc
      541 ttgggaacac tgatttactg gctgcccaaa gacggcaaca ttgctttcaa tgccttcaca
      601 gcggtgttga ttgtggcttg tccctgtgcg gtggcattgg cggtaccttt cattttcgga
      661 aatgtactcc gcattctggc ccgcaacaaa ttttacctca aaaacacctc cgtcattgag
      721 tccattgagg aagccgatgc ggtggtattc gataaaaccg gcaccatcac gcagcgccag
      781 aacagcgaaa tctggttcgn nnnnnnnnnn nnnnnnnnnn ngataggtct tggcggtgat
      841 gcgcgagggc ttgccgaaca tggtgtcgcc cagggccagc acggccagcc cgttgacctg
      901 gccgaccacc gcgccgaacc tctgaactgg cacgaggcta ccgggcaagg cgtagaagcc
      961 gtgttcgggc aacgcaccct gcggctcggc tccaaagact tcatcgggcg cgacatacaa
     1021 gaagtattgc acagcgatga tgccggcgta tttttacaaa tcaacgacag cctgcgcggc
     1081 tatttccgca tagaccagcg cttccggccc ggcacgcagg aggcattgca gttcttctcc
     1141 caacaggcgc ccatttacct gctttccggc gacaacgacc gcgaaaaaga atcggtgttg
     1201 cctttgttcg gttcggaaga ccggctgcgc ttttttcaaa gtccgcagga taaattgcag
     1261 tttgtcaaag gattgcaaaa cgaaggccgc aaagtgatga tgctcggcga cggcctcaac
     1321 gacgcgggag cactccagca aagcaatgcc ggtattgtac tggccgagga taccaacaac
     1381 tttacgccgg cctgcgatgc gatcctgcac tcggcgcaat tcaggcggct gcccgacttt
     1441 gtgcagttgg tgcgtcgcag cacgaaattg gtgtattacg cctacggatt ggctttcgtt
     1501 tacaatgtca ttggtttgag ctatgcggtg caggggctgt tgtcgccggt ggtggcggcc
     1561 attctgatgc ccacgagcag catcaccatt gtggtgttcg ggatgttgtc gagtaattgg
     1621 ctggggcgga ggttgagatt gtgagtttta atcagagttg ctttaatcgg agaaaagcat
     1681 tggctttagc cagacgaaaa atcaatcccc cgccactctt tcaccaagca gcgggggatt
     1741 tccattttaa tgctttacca ttcgcccgta aaaacgctca ctatcggtat ccagcaacat
     1801 gaaatagatg cccggcgaca ggcgtgaaac gtttacttcc agatagttgt gtcccgattg
     1861 caggctgtgg gatgttgttg aaacaagacg accatccaca tcggtgatgc gcagggcagc
     1921 agaagtggag gcttcccggc atgaaatctc caaacccgcc cgctcggcag tcggattggg
     1981 aaaaacgcgc atttcgatgg ctccattaga cgattccggc ttcaccgctg aagaaatcaa
     2041 cccggtttcg taaatatcca atgcccaggg ctgccaatgg ctgatccaca accggttgtg
     2101 agcatctaaa ccaagtccgt gcatcgctcc gccggacagc ggcgagttgg tgggattgaa
     2161 ctcttgccat tggccctcgg cgtacagcaa aaccctttcg gaagaagaca tggcggcaaa
     2221 cccgtcgcgg gcatgcacgc gcattatcca ctgatgcccg gcagcaggca acggaaacga
     2281 ctcggtatgc cagatactgc cgtcgaaata ttgagcatgc tctacattgc cggtcaccca
     2341 taagcgttga taatcagcgt cataagccaa agcaagcgca tagtccatgt cgaagccggc
     2401 cacaccctgc gtgaaagtct gcatatcggc gccnnnnnnn nnngttgaag cggcctaccg
     2461 tgagcgactg cccgttgagg taagaagaga accagagatc gccattggcg ccgccggtca
     2521 tggcatacac atagtaatta ctgaacaaag gatgagggta tcgcatccag ttcgagccgt
     2581 caaaacggta gatcgcgtcc tgataaacat agacccagac gcgccccaga tcgtcaacgg
     2641 taatgcgcgt aagcagcttt tgagcgggca ggagcgagtt ggtgtggtcg taacgcgtcc
     2701 attgccccag ttggtagtga tataggtgga agccggaagt cgcccagatg ctgccatccg
     2761 gagcaaaaga gaagttgccg accgcaaaag actgcccgtt gaaaatacat ggaatcgctg
     2821 cgccggaatc caaattgagc agcctgcggt cttcagtgac cgtccaaagt tgtttctgat
     2881 ggtcggtttg aaagcgttct ggcagcgact caaagggata attcaacaat gccgccatag
     2941 cggtatcctg agcacccggt atgagtattt ctttggaatt cagaatgatg tcaaagtcct
     3001 cgctcctata cgcgccgatg atggggagcg cgccggtgta ggtactctcc agcgtccatt
     3061 cgccgttgtg gaagcgcaaa aaccggaggt tgtcggagta atagatggaa aggcttccat
     3121 ctggtagttc attgataatc tgtggttttc tatgtccaac gcctactgat tgagccggat
     3181 cgtacaattc ccatgcgcca ttatgaaaac ggcctgcgcg cccgatgtcg gtaaaaaacc
     3241 attgatcgcc attggaggct gtaaaggctt tggcatcgcc gtaagcaaaa aggaaaaagc
     3301 cgttattgcc agagggcgag ggggaaaata attgaggcgt ctcttcgccc tgcggcacct
     3361 tgtacatcac gttcgaggcc gccagaatag cgccgtcttc ggttttggcc atgctccaga
     3421 ctgcacccaa aaaagaaagg ccggctgact ggttgaacag cgtccactca ccatcggtat
     3481 agcgggccaa tcctacgctg gtacccgccc agagcctgcc ttcgttgtca aattccatgc
     3541 aataggtgag cagcatattt ccgcccgcct gattgcgaat aaacgccgga tagtcaatgg
     3601 attgccactg accatcaggc gagcgaaagg ccagcgcaac gtcataagtg cctataaatg
     3661 gctgaagtgt ttgtggatca atcgtcagcg cctctactga gttggaaggc agcccggcat
     3721 tggctttggt cagatgctct gctacttgtc cggttgaggg gtttataata aaaatacccg
     3781 catcggtggc ggcccaaagt tggccttccc actccatcca gtcgttgact ttggaaggaa
     3841 tggggtaagt gtgccaggtt ccggcttgcg ccaaaagcga gatattcgtc agtgtggcgg
     3901 ccaagagcag taaaaaagac atcattcgat tcataacgaa gtgtttttca atgtgtgaaa
     3961 gtacattagt agtggggcaa aataaatgtt cattttcttt aggccaaagg attagataag
     4021 acacttaaaa tga
//
LOCUS       DAORLF010000434         4010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12239_length_4010_cov_2.042731, whole genome shotgun sequence.
ACCESSION   DAORLF010000434 DAORLF010000000
VERSION     DAORLF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12239_length_4010_cov_2.042731"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..742
                     /locus_tag="PK198_23330"
     CDS             95..742
                     /locus_tag="PK198_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRF41754.1"
                     /translation="MKQTFIFDMDGTMVDNMMVHHRAWQRKLAALGLDMTLEEVRQSI
                     HGINEEILERLFGSRFSPEERRRIAWEKEAEYRDIFLSELKLVPGLEAFLKSAQEAGI
                     RMGIGTAAPHENVQFVLDHLHLQPLFGSVIDASQVTKGKPDPEVFLRVATELGAEPAD
                     CIVFEDSPTGAEAARRAGMKAVIITTTHTADEFAHFEHVVRVISDFEGLKPEDFG"
     gene            complement(823..2025)
                     /locus_tag="PK198_23335"
     CDS             complement(823..2025)
                     /locus_tag="PK198_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41755.1"
                     /translation="MMNLRMKPETMALLLASALFAASCMSPEKLVETGDYDRVIEMSM
                     DKLAGRAKKNPRYVDALSQAFERANDRDLAEAEALRRDNRPEHWDRILSLYQRVADRQ
                     NRVAPLMPLADKHGIRADVRFVQVEDLIRDARNNAAEYHYNRALELMAQARLGDRFAA
                     RNAYDALSVIDRYFRNYQDKEALKNEALHLGKTHILLSVRNEAQVLLPEYLDAELRRL
                     PLRDLDSRWRQYHTRPLAGVQYDYEVVVNMVNITATPEMVREREYEDVCEVEDGFEYI
                     LDSRGNVMKDTAGNDLKVPKKKLVRARVFETWQQKTATLHCRVDFYDKYNNNALIDSW
                     PITADAVFENYASTFSGDQRALSDQSRNRLGNRPLPFPSDEALLMQAADRLKPIIKNR
                     ISKARVTT"
     gene            2413..2643
                     /locus_tag="PK198_23340"
     CDS             2413..2643
                     /locus_tag="PK198_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRF41756.1"
                     /translation="MKNYREIITIHPDIRFGKPCIRNTRITVGDVLGWLAAGMSYEEI
                     LEDFPELSLEAIRAALAFAADREESVKILAAS"
     gene            2839..>3287
                     /locus_tag="PK198_23345"
     CDS             2839..>3287
                     /locus_tag="PK198_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HRF41757.1"
                     /translation="MSDMITNRGVSLSQVSDGSPVLLIFLRHFGCTFCREALADISDL
                     REDIERLGVRIIFVHMTDNDTAEPFFHRFGLDGAEHISDPMCQYYAAFGLVKGSYRQL
                     YGLRSWIRGFQSIVVEGNGYGAELGDGFQMPGIFVIYQRNVRGSFIH"
     assembly_gap    3288..3328
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3375..>3633)
                     /locus_tag="PK198_23350"
     CDS             complement(3375..>3633)
                     /locus_tag="PK198_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41758.1"
                     /translation="FANLSPYVEADELEMKRAAIESIMDEVLDHLDASRKGAFKSAIS
                     EAEIGIRALLRSIHREKDRSREFFKEGKEISSFLKTAASLL"
     assembly_gap    3634..3713
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3714..>4010)
                     /locus_tag="PK198_23355"
     CDS             complement(<3714..>4010)
                     /locus_tag="PK198_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41759.1"
                     /translation="FIASQYIWKHELARAMERHEQREASIIPIFIKYCDWKGGPFDKL
                     QGLPKDAKPVGRADNDMAWREVAQGVKAVAEAILSGTPIPLFPPLTTEPTTLHQP"
BASE COUNT          896 a          925 c         1083 g          985 t
ORIGIN      
        1 gcggacaggt tttagcgaag ggcgcgaaat tccgcgaacg tagggtggca acccgaaacc
       61 ttgaacccga attttttcaa tccgaaattg actcatgaaa caaacattca tttttgacat
      121 ggacggtacc atggtggaca acatgatggt gcatcacagg gcctggcagc gcaaactggc
      181 tgcgctgggt ctggatatga cgctggagga agtacgccaa tccattcacg gcatcaatga
      241 ggagatattg gaacggctgt tcggcagtcg tttttcgcca gaagagcgcc gccgcattgc
      301 ctgggaaaaa gaggccgaat accgcgatat ttttctgtcg gaactgaaat tggtgcccgg
      361 attagaggca tttctaaaaa gtgctcagga ggcgggcatc cgcatgggca taggcacggc
      421 ggcgccccat gaaaatgtgc agtttgtgct cgatcatctg catttgcagc ccttgttcgg
      481 ctccgttatt gatgcctcgc aggtgacgaa aggcaagccc gatccggagg tttttttgcg
      541 ggtagcgaca gaattaggcg ccgagccggc agattgtatc gtgtttgaag attcgcccac
      601 cggagcagaa gccgcacgcc gggccggtat gaaggccgtc atcatcacta ccacacatac
      661 tgccgatgaa tttgctcatt ttgagcatgt tgtgcgggtg atttcagatt ttgaggggct
      721 gaaaccggag gattttgggt aacgaaaaaa taaaaaacac cgacgaagac gcgccccgcc
      781 ggtgttcaaa atgtttgcag atcaatgttt cttatcggga tatcaggtgg tcactctcgc
      841 tttggaaatc cggtttttga tgatgggctt gagcctgtcg gcggcctgca tcaagagcgc
      901 ttcgtcgctg ggaaaaggca gcgggcggtt gccgaggcgg ttgcgtgact gatcgctgag
      961 ggcgcgctga tcgcctgaaa aggtggatgc gtagttctcg aatacagcat cggcggtgat
     1021 aggccaggaa tcaatgagtg cgttattgtt atacttgtcg tagaagtcca cgcggcagtg
     1081 gagggtggcg gttttctgct gccaggtttc aaatacgcgg gcgcggacca acttcttttt
     1141 cggcactttc aggtcgttgc cggcagtatc tttcatcaca ttcccacggc tgtccaggat
     1201 atattcaaac ccgtcttcca cttcgcacac atcttcgtac tcgcgttcgc gtaccatttc
     1261 gggcgttgct gttatgttga ccatgttcac caccacttca taatcgtact gtacgccggc
     1321 caatgggcgg gtgtggtact gacgccagcg gctgtcgagg tcgcggaggg gcaatcggcg
     1381 caattctgca tctaagtatt cgggcaggag tacctgcgct tcgttgcgca cgctgaggag
     1441 gatatgcgtt ttgccaaggt ggagcgcctc gtttttgagg gcctctttgt cctgataatt
     1501 gcggaagtag cggtctatga cggagagcgc gtcgtacgca ttgcgggcag cgaaacggtc
     1561 gccaaggcga gcctgtgcca tcagttccag cgcgcggttg tagtggtatt cggcggcatt
     1621 gttgcgggca tcgcggatga ggtcttctac ctgcacaaag cggacatcgg cgcgaatgcc
     1681 gtgcttgtcg gccagcggca tcaggggcgc cacgcggttt tggcggtcgg ccacccgctg
     1741 atacaggctg aggatgcggt cccagtgttc ggggcgattg tcgcggcgga gggcttcagc
     1801 ttccgccaga tcacggtcat tggcacgctc gaacgcttgg ctgagggcat ccacatagcg
     1861 gggattcttt ttggcgcggc cggccaattt gtccatagac atttcgatga cgcggtcgta
     1921 gtcgcctgtt tccactaatt tttcggggga catacaagag gctgcaaaaa gagccgacgc
     1981 caacaagagc gccattgttt ccggtttcat tcgcaggttc atcatggctt ttcgatttta
     2041 acaggtgact aaatcaggtg cctcatttgg ttgttttgat gatgcgaagt taatccctcc
     2101 caaaggcgag gtaagctaaa gaagcgttaa tccggcgccg ggaaatcgtt aaattgtgtc
     2161 aagaaggaag aggtggagtt ggtgggtgga ttgcttaact tgttgtttat gagtttttta
     2221 taaataaatt tcttcttaaa aaagggttaa gaggtttgca gaggagtgag gggaaattgc
     2281 ccggcatgaa actgaactca ggttcctgct catagcggga tgccaatgac tcaatagcag
     2341 tgggaagcat taggcaattc aacccttgtc tccatttgta tgttgtcatc gaatgtatcg
     2401 aacagcatta gtatgaaaaa ctaccgcgaa atcatcacca tccaccctga catccgtttc
     2461 gggaagccgt gtatccgaaa tacccgcatc acggtgggtg acgttctggg ctggctggcc
     2521 gccggaatga gctatgagga gattttagaa gactttcccg aattgagttt agaggccatt
     2581 cgggctgcgc tcgcttttgc agcagacaga gaggaaagtg taaaaatcct ggcggcttca
     2641 tgaaaatctt gctggattcc gcaaaaatag agacccttga aaacagcgcg cactatggtt
     2701 tgttggaaat ctactgagcg agagacgaga ctatacacaa tgcctcattt ttatgaactt
     2761 tcaccaaccc gctttgtttt gaaacggtcg aaaaagcaag aatagcggat ggaatttgta
     2821 ttgaacaacc cggaggtaat gtccgacatg atcaccaatc gcggcgtttc cctgagccag
     2881 gtctccgacg gcagccctgt gttgctcata tttctgaggc attttggctg caccttttgc
     2941 cgggaagccc tggccgatat ttccgacctc agagaggaca tcgaacgcct cggcgtccgc
     3001 atcatcttcg ttcacatgac cgacaacgac actgccgaac cgtttttcca tcgtttcgga
     3061 ttggacggcg cggaacacat cagcgacccc atgtgccaat attacgcggc tttcggtttg
     3121 gtaaagggca gttaccggca gttgtacggc ctgcgctcct ggatacgggg ctttcaatcc
     3181 atcgtagtag aaggcaatgg ctatggcgcc gaattgggcg acggcttcca gatgccgggc
     3241 atttttgtca tctatcagcg aaatgtgcgg ggcagtttca tccacaannn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt agagggtatc aggaatggaa cataaacatg
     3361 acaggaggtt atactcataa taacgatgct gccgtcttca gaaaacttga tatttcttta
     3421 ccctctttga agaattctcg cgaacgatct ttctctctat ggatgcttcg caataacgcc
     3481 ctgattccta tctctgcttc cgatatagca gatttgaatg ctcctttcct tgaagcatct
     3541 aaatgatcta gcacttcatc cataatggac tcaatcgctg ctcttttcat ttccagttca
     3601 tctgcttcca cataaggaga caagttggca aaannnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggttgg
     3721 tgtagagtgg taggctcagt ggtgagaggg ggaaacagtg gaatcggggt gccggatagg
     3781 attgcctcgg ccacagcttt aaccccttga gctacttctc tccaagccat gtcgttatca
     3841 gccctgccga caggctttgc atctttaggc agaccttgta atttgtcgaa cggcccccct
     3901 ttccaatcac aatacttaat aaaaataggg atgatgctgg cttcccgttg ctcgtggcgc
     3961 tccatagcgc gggcaagttc gtgtttccaa atatattgag atgcaatgaa
//
LOCUS       DAORLF010000435         3997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12293_length_3997_cov_2.537291, whole genome shotgun sequence.
ACCESSION   DAORLF010000435 DAORLF010000000
VERSION     DAORLF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_3997_cov_2.537291"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>607
                     /locus_tag="PK198_23360"
     CDS             <1..>607
                     /locus_tag="PK198_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41760.1"
                     /translation="LLGIALTFSLISCLQAQKAPGYMGRRLFIRPEIAYAPALLGPTA
                     NNRGGADYIYGEQGGGFGFNAKYGVQAGYAISRSEVLSFEGSYMATGMIMEASTPSIL
                     FQNNSDQHYLFYKLSGPEIGLAWNVYNVNNGSIAPIGSYVTVRARMAFLSGTVLDKRT
                     TYYRSNSSAGHLPLGVIPRYRHLSLGAEVGRQMMIGNRMVLS"
     assembly_gap    608..622
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            808..1701
                     /locus_tag="PK198_23365"
     CDS             808..1701
                     /locus_tag="PK198_23365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41761.1"
                     /translation="MTGVKTTLWPGFAMLLLAILTGCAPYEVQFEGPYDDSKNTAGSY
                     IPNEILTVENGQIRLYPINLTEGKTLSNLPAGIEKASINHAHTRIAYKIPGQNIVIID
                     TAGASIDVVANSAAASWFDWHVNNQTLYMLNGTTLSFWGPAVSVISTNLSNFFPQGST
                     DRTMSTVAIAADGTVVATYRYFSGSSVGRMGWVSLAGAVKAVNLTSSNETGRWMRVSR
                     EGDIILCGTTFGTTSINNFRLNLTQDVVTQPTARSFAAPGPSNDLLVYATSGKLNISD
                     TNSGTVLNRNINTASLTALDW"
     gene            1736..3973
                     /locus_tag="PK198_23370"
     CDS             1736..3973
                     /locus_tag="PK198_23370"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF41762.1"
                     /translation="MKTTLLFILSLLLAPLFLTAQNEYAPLECQGKIPKDFLTTSHSK
                     YQNADKNVLAADAKRTERNAFKQFQLATNFYLDQLLHSGRVLFNDEASQYLADVTQVL
                     LNAQPTPELQNVRVYALRSSAVNAFATDRGEVFVTLGLLAQLENEAQLAYVIAHELVH
                     VQKRHSLKFHLDKQKLQRNQGRSILGRAATDEQLFTAATYSKENENEADRLGLERILL
                     SRYNTATLPVVYDVLKYSYLPFDDLPFEYTLLESEHYRLPPAYRLESVKPIEGEADDE
                     DDSRSSHPNLGSRRQAMLATLDALTDAPEKTDYVVSADRFARVRALARNEMPLLYLHS
                     DEHAAAIYNAGLLLRERPDDVGLKKCIAKALYLHAKLRNSEDYSYEGNYEEIEGESQQ
                     LHYLLETIPTAESTVLAWQYAWNLHRAHPDDAELKAITTDLFFEFIREYGKQYASLSN
                     ELPTAETADATPAPDSAAVQPKSKYDRIRAQKSATTVSENNYWQYAFVGQMEDELFLS
                     YKKSADERLREWKINERFYTTAAGRRELKKLRTQGWALDIPKIVVVNPFYIKMDERKE
                     ETVQYVDSEIGQQHFRDLIKETADASGLQTTLMDVHDLKEDDTETFNDLRFLNEWFSE
                     QACHFDLSLTPGNQQARIDSIAQKYDTDYFLWTGLVSLRDKHTTLEWLAAVTSIVVVP
                     PIAPYLLYKVLTPRYVTVYYSILYDVRTGRRSVLKMNTYTTRASDTMVKAHLYDAFWQ
                     IKRKG"
BASE COUNT          985 a         1111 c          967 g          919 t
ORIGIN      
        1 cctgctcgga attgcgctga cattcagcct catttcctgt cttcaggccc aaaaagcgcc
       61 cggctatatg gggcggcgtt tatttattcg tcctgaaata gcttatgctc cggcgctgct
      121 ggggcctacc gccaacaaca ggggcggcgc tgattatatt tacggcgagc agggcggcgg
      181 ttttggtttc aatgccaaat acggggtgca ggccggctat gctatttccc ggtctgaagt
      241 gctgtcgttc gaaggcagtt atatggctac aggcatgatt atggaggcat caacgccgtc
      301 tattcttttt caaaacaatt ccgaccaaca ctacctgttt tacaaactca gcgggccgga
      361 gataggtctg gcctggaatg tgtataatgt aaataacggc agcattgcgc ctatcggcag
      421 ctatgttacg gtgcgtgcca gaatggcctt cctttccggt accgtccttg ataagcgcac
      481 cacctattac cgcagcaact catcggcagg gcaccttccg ctgggcgtca tcccccgtta
      541 tcggcacctg tcactgggag cagaagtggg ccgtcagatg atgatcggga atagaatggt
      601 gctctctnnn nnnnnnnnnn nnctgtctat ggaggtgaac gttgagcctt tcaatttact
      661 ccgcctcctg ctgttagagg actacacttt cgaaaccaac caacaggttt tccacaatgc
      721 agcctacgcc cgaatgttcc ggcattcatt ggtcatgttt aaaaccggag tggggtatct
      781 tttttaataa ccataacaac aacaaccatg acaggcgtaa aaacaacgct gtggcccgga
      841 tttgctatgc ttctgctggc cattttgacc ggctgcgcgc cctacgaagt gcaatttgaa
      901 gggccttatg acgacagcaa aaacacagcc ggatcgtaca ttccgaacga aatcctgacg
      961 gtagaaaacg gccaaatccg gctttatccg atcaatctca cagagggcaa aacgctctca
     1021 aatcttccgg ccggcatcga aaaagcctcc atcaatcatg cccacacccg cattgcctac
     1081 aaaattccgg ggcaaaacat cgtcatcata gacactgccg gagcaagcat tgacgtcgtg
     1141 gccaactcgg cggccgcttc ctggttcgac tggcacgtca acaaccaaac gctgtacatg
     1201 ctcaacggca ctacgctttc tttttggggg ccggccgtct ctgtcatttc caccaatctg
     1261 agcaactttt tcccgcaggg ttctaccgac cgcaccatga gcaccgtagc tattgccgcc
     1321 gacggcaccg ttgtggccac ctaccggtat ttcagcggca gttctgtggg gcgcatgggc
     1381 tgggtgtcgt tggccggcgc cgtaaaggcc gtcaatctga ccagcagcaa cgagacgggg
     1441 cgatggatgc gcgtgagtcg cgagggcgat atcattttgt gcggaactac cttcggcaca
     1501 acgagcatca acaattttcg actcaatctg actcaggatg tcgtaaccca acctactgcc
     1561 cgttcctttg ccgctcccgg tccctcaaat gatttattgg tttatgcgac aagcggtaag
     1621 ctcaatattt ccgacaccaa ttcgggtacc gtactgaatc gcaatatcaa tactgcttcg
     1681 ctcactgcat tggactggta gtatgacaac gttacacacg cacactcttt tttctatgaa
     1741 aaccactctc cttttcattc tctccctgct gctggcgcct ctttttctga ctgctcaaaa
     1801 cgagtacgct cctttggaat gtcaggggaa aatcccgaaa gattttctga caacttctca
     1861 ttccaaatac caaaatgccg acaaaaatgt gctggccgcc gatgccaaac gcaccgaacg
     1921 gaacgccttc aagcagtttc agttggccac caatttttat ttagatcaac tgctgcacag
     1981 cggccgggtg ctgttcaacg atgaggcctc gcaatatctg gccgatgtca cgcaggtttt
     2041 gctcaatgca cagcccaccc cagaactgca aaatgtgcgc gtgtatgcct tgcgcagcag
     2101 cgccgtcaat gcttttgcca ccgatcgggg ggaggtgttc gttacgctgg gcctccttgc
     2161 tcagttggaa aacgaggcgc aattggccta cgtcatcgcc cacgaattgg tacatgtgca
     2221 aaaaaggcat tcgctcaaat ttcacttgga taagcagaaa ctgcaacgca atcagggccg
     2281 cagtatattg gggcgggcag ctaccgatga gcaattgttt acggcggcca cctattccaa
     2341 agaaaacgaa aacgaagccg accgcctcgg attggaacgc atccttcttt cgcgctacaa
     2401 caccgctacg ctgccggtgg tgtacgacgt actcaagtac agttacctgc cttttgacga
     2461 tttgcctttt gagtacaccc tgctggaatc ggaacactat cgcctgccgc cggcctatcg
     2521 cttggagagt gtaaaaccca tcgaaggcga ggccgacgat gaagacgaca gccgcagttc
     2581 gcaccccaac ctcggttcgc gccggcaagc catgctcgct acattggacg ccctcactga
     2641 cgcgccggaa aaaaccgatt acgtcgtgtc ggcagatcgt tttgctcggg tgcgcgccct
     2701 ggcccgcaat gaaatgccgc tcttgtacct gcactccgac gagcacgccg ccgctattta
     2761 caatgccgga ctgctgttgc gggagcgccc cgatgatgtc ggactgaaaa aatgtattgc
     2821 caaagcgctg tatctccatg ccaaattgcg aaacagcgaa gactacagct acgaggggaa
     2881 ttacgaagaa atagagggcg agtcgcaaca attgcactac ctgctggaga ccatccccac
     2941 tgccgagagt accgttttgg cctggcaata cgcctggaat ctgcatcggg cgcaccccga
     3001 tgatgccgaa ctgaaagcta ttacaaccga tttgtttttt gagtttattc gggaatacgg
     3061 caaacagtat gcctcgttga gtaatgagct gccgaccgcc gaaaccgccg atgcaactcc
     3121 cgccccagat tccgcagccg tgcaacccaa atcgaaatac gaccgcatcc gggcacaaaa
     3181 gtctgccact accgtgagtg aaaacaacta ttggcaatat gctttcgtcg ggcagatgga
     3241 ggatgaactt tttttgagct acaaaaagag tgccgacgaa cgcctgcgcg aatggaaaat
     3301 aaacgagcgc ttctatacta ctgctgccgg gcgcagggaa ttgaaaaaac tacgcacaca
     3361 gggctgggcg ctggacattc ccaaaatagt ggttgtcaat cctttttaca tcaaaatgga
     3421 tgaaagaaaa gaggaaacag tgcaatatgt ggactcagaa atcggccagc aacacttccg
     3481 tgatctcatc aaagagaccg ccgacgcatc tgggctgcaa accaccctca tggacgtaca
     3541 cgacctcaaa gaagacgata cggaaacctt caacgatctt cgtttcctta atgaatggtt
     3601 ctcggagcag gcctgccatt tcgacctcag cctcactccc ggcaaccaac aggcgcgcat
     3661 tgattccatc gcccaaaaat atgataccga ttatttcctg tggacgggcc tcgtttctct
     3721 tcgtgacaaa catactacct tagaatggct cgctgccgtg acttccattg tagtggttcc
     3781 tccaattgcg ccatatttgc tatacaaagt attgacaccc agatatgtta ctgtgtacta
     3841 ttccatcttg tacgatgtgc gcaccggtcg ccgtagtgtc ctcaaaatga atacatacac
     3901 tacccgcgcc tccgatacaa tggtcaaagc ccatctgtac gacgctttct ggcagatcaa
     3961 aagaaagggg tagggaggga taagtttcgg gttcaag
//
LOCUS       DAORLF010000436         3993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12315_length_3993_cov_2.236668, whole genome shotgun sequence.
ACCESSION   DAORLF010000436 DAORLF010000000
VERSION     DAORLF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12315_length_3993_cov_2.236668"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..298
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <299..2813
                     /locus_tag="PK198_23375"
     CDS             <299..2813
                     /locus_tag="PK198_23375"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRF41763.1"
                     /translation="LRLPFHGIETTGAKLALFSQECNNGLENGLTPTELIEAFLGEQS
                     SQKSISERYDLLFRFIQYAERQVVLFDSIEDASFAETHDLEGKGSVKHLLGRINDEGR
                     RKRLLEKLLNYSVRVVLTAHPTQFYPGKVLGIIHDLGKTIRRGDVEEVHQLLMQLGKT
                     AFVNRAKPTPLDEALSLNWYLANVFYEALPQSVWNLLKGLGLEPHSFSNPRLLTLGFW
                     PGGDRDGNPFVTAETTREVARRLRRTILGCYYRDIRQLRRRLTFRGVEEQMAAIESRI
                     YHTLYYPDKPHYAACFQLIDDLMNARQALVNEHQGLFSDALDLFLLKVRLFGFYFAEL
                     DVRQESGRHEEVLQPILQKLEQKFPALFRMADYEAMPESQKMDFLLTWRDPLDESDYD
                     DEQVKESIRTFKTIQAVQRENGEQGCRRYIISNCGSALEVAQVFALARLCMDGTDAPT
                     LDIVPLFETVEDLSTAHQTMETLYRHPQYAAHLEKRGKVQTIMLGFSDGTKDGGYLRA
                     NWSIYRAKEALTLISRQYGISVVFFDGRGGPPGRGGGNNASYYAAHGSEIENREIQVT
                     IQGQTVSSTYGTVEAAMYNIEQLFTAGLEKTMSPGAGNMMETDKTLLDELADAAYEAY
                     LQLKNDPDFVPYLEQMTPLKWYGDTNIASRPTKRNGDNTSLVFKDLRAIPFVGAWAQM
                     KQNIPGYFGFGKALEDIIAQGKLTQLQQLYRESRFFRALAENSMQSLRKSNFLLTRHL
                     RRHERFGHFWEQLYAEFQRTQKLLLQISGEPELLAKNPVSRESIRIREGIVLPLIVIQ
                     QYALERLNNNNLSEQESEIIRHLVLRAMFGIINAGRNVA"
     gene            complement(2963..3424)
                     /locus_tag="PK198_23380"
     CDS             complement(2963..3424)
                     /locus_tag="PK198_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41764.1"
                     /translation="MKQYGLPFENKYSIKDKKILLDGIGRIGFTTRDSFLVLEKDGCK
                     LTFVKKNQDETLSEDSLSSIAFYVSDDEGVLLDSVQIHKTSSDAHIFRIAGSIEKKYL
                     DKTFDERVLDTNEYKIILHLRNGKVFQITSRGKYETPFEVQTLIKYLLAEI"
     gene            complement(3721..>3993)
                     /locus_tag="PK198_23385"
     CDS             complement(3721..>3993)
                     /locus_tag="PK198_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HRF41765.1"
                     /translation="KYVEMNVAHPCMEGNGRSARIWLDLILKKHLKKCVDWSKIGKNE
                     YMNAMIKSPVNSSALKKLLKNALTTEIDSREMFMKGIDYSYYYEEN"
BASE COUNT          980 a          992 c          952 g          985 t
ORIGIN      
        1 actggggctg agtctggcgc tgggttcccg caccgatgtg ccgctcgatt tcagcgcgcc
       61 caaagtgcat gtgggatatg tggggttgtt ttaacagagg tgagggatga agggtgagag
      121 gtgaggggtg aatagttggc aaaaaaaacg cctgaaaacg cgcaaagtgc cgggcagcaa
      181 cgatatttgc gcgcccaacc aatgagaccg ctcannnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
      301 tgaggttgcc tttccacggt attgaaacga ccggcgctaa gctggcactc ttctctcagg
      361 aatgcaacaa cggccttgaa aacggactga cgcccactga actcatcgag gcgtttctgg
      421 gggagcagtc ttctcaaaaa agcatctccg aacgatacga cttgctgttt agattcatac
      481 aatatgctga acgacaggtg gttttgttcg actctatcga agatgcctct tttgccgaaa
      541 cgcatgatct ggagggcaaa ggctcggtga aacatctgct gggccgcatc aacgatgaag
      601 gacgccgcaa acgcctgctg gaaaaactgc tcaactattc cgtgagggtg gtgctcaccg
      661 ctcaccccac gcaattctac cccggcaaag tactgggcat catccacgat ctggggaaaa
      721 ccattcgccg gggcgatgtg gaagaagtgc atcaacttct catgcaattg ggaaaaacgg
      781 cctttgtgaa tcgcgccaag cccacgcctt tggacgaagc cctgagcctc aactggtatc
      841 tggccaatgt attttatgag gcgctgccgc aaagcgtgtg gaatctgctc aaaggcttgg
      901 gattggaacc acacagtttc agcaatccga ggttgttgac actgggtttc tggcccggcg
      961 gagaccgcga cggcaatccg tttgtaaccg ccgaaaccac ccgcgaagtg gcccggcgcc
     1021 tgcggcgtac catcctgggc tgttactacc gggacatcag gcaactgcgg cggcggctca
     1081 cttttcgggg cgtggaagag caaatggccg ccatcgaaag ccggatttat cacaccttgt
     1141 actaccccga caaaccgcat tatgcggctt gttttcaact gattgacgat ttgatgaacg
     1201 cccggcaggc tttggtcaac gaacatcagg gtttgttttc ggatgcgctc gacctctttt
     1261 tgctcaaagt caggcttttc ggtttttact ttgccgaatt ggacgtgcgg caggaaagcg
     1321 gtcggcacga ggaagtacta cagcccattt tgcaaaaatt ggaacaaaaa tttcccgcgc
     1381 tctttcggat ggccgattac gaagccatgc ccgaatctca aaaaatggac ttcctgctga
     1441 cctggcgcga ccccttggat gaatcggact acgacgacga gcaggtgaaa gaaagcatcc
     1501 gcacgttcaa gaccatacag gccgtgcagc gcgaaaacgg cgagcagggt tgccggcgct
     1561 acatcatcag caattgcggc agcgctttgg aagtggcgca ggtgtttgcg ctggcgcgcc
     1621 tgtgcatgga cggcaccgat gcccctacct tagacattgt gcccttgttt gaaacggttg
     1681 aagacctttc aacggcccat caaacgatgg aaaccctcta ccggcacccg caatatgccg
     1741 ctcatctgga aaaacgcggc aaagtgcaaa ccatcatgct gggcttctcc gacggcacca
     1801 aagacggtgg ctatctgcgg gccaactgga gtatttaccg cgccaaagaa gccctcacgc
     1861 tcatcagcag gcagtacggc atttcggtgg tgtttttcga tggccggggc ggccctccgg
     1921 gtcggggcgg cggcaacaac gccagctact atgcggcgca cggcagcgaa attgaaaacc
     1981 gcgaaattca ggtgacgatt caggggcaaa cggtgagcag cacttacggc accgtcgagg
     2041 cggccatgta caacatcgag cagctcttca ccgccggcct cgaaaaaacc atgtcgccgg
     2101 gcgccggaaa catgatggaa accgataaaa ccctgttgga cgagttggcc gacgccgctt
     2161 acgaggctta tctccaactg aaaaacgacc ccgattttgt gccttacctc gaacaaatga
     2221 cgccgctgaa atggtacggc gacacgaaca ttgccagcag gcccaccaag cgcaacggcg
     2281 ataatacttc tttggttttc aaagatttaa gggccattcc cttcgttggc gcctgggcgc
     2341 agatgaagca aaacattccc ggttatttcg gtttcggaaa agcattggag gacatcatcg
     2401 cacaaggcaa actgacgcaa ttgcaacaac tttaccggga gtcgcgcttt ttcagggctt
     2461 tggccgaaaa ttccatgcag tctctgcgca aatccaattt tctcctcacc cggcacctgc
     2521 gccggcacga gcgcttcggg catttctggg agcaactcta cgcagagttt cagcgcacac
     2581 aaaaactgct gcttcagatt tccggcgaac cggagctttt ggccaaaaat cctgtctccc
     2641 gcgagagcat ccgcatccgg gagggcatcg ttttgcccct catcgtcatt cagcagtacg
     2701 ctcttgaacg actgaacaac aacaacttat ctgaacagga aagcgagatc atccgccatt
     2761 tggtactgcg ggccatgttc ggcatcatca atgcgggcag gaatgtggcg tgaggggagg
     2821 tgcaaggttt cgagggtagt gaaggggtga ctgcggggct aaaacttgca aagtcacccc
     2881 ttcactaggc tggagaagaa cgctctgcgc ttgcaccgcc cactgcatga aaatgaaatc
     2941 tcggtgcgat ctgctttgca ctttaaattt cggcaagcaa atattttatt agggtctgaa
     3001 cttcaaatgg ggtttcgtat tttccacggg atgttatttg aaagaccttc ccgtttcgga
     3061 ggtgcaaaat tattttgtac tcatttgtgt ctaaaaccct ttcatcaaat gtcttatcaa
     3121 gatatttttt ttcaatactt cccgctattc tgaaaatatg ggcatctgat gatgtcttgt
     3181 gtatttgaac agagtcgagc aaaacccctt catcatcact cacataaaaa gcaatacttg
     3241 acaaactgtc ttcagatagt gtttcatctt ggtttttttt tacaaatgtg agtttacacc
     3301 catctttctc aagtactaaa aatgaatccc ttgtcgtaaa ccctattcgt ccaatgccat
     3361 ccaaaagtat ttttttgtct ttgatactgt atttgttttc aaaaggcaag ccgtattgtt
     3421 tcattgaagg gttataaaac agcgaactgg ttagatatat tttatgtcca tacttatggt
     3481 atggatacac aaaagggttt agttccaaaa aatttttgtc gcaatcatcg ctgttcttta
     3541 attgccaaaa tccggatatt tcacttttgt ccccattaca ttttaaagac aaaaaactca
     3601 gcaaacacaa acaaaacatt agcatcataa ggtgtctcat catcatacca tcttgtcttt
     3661 agaagatacg ccatcgcggc ctatgagttc cgttcttgtt tctactccgt tcattatttt
     3721 ctaattttct tcgtaatagt aggaataatc aattcctttc ataaacattt ctctgctgtc
     3781 aatttcagtg gttaaagcat ttttgagtag ttttttcaaa gcgctactgt ttacagggct
     3841 ttttatcatg gcattcatgt attcgttttt cccgattttg ctccaatcca cacatttttt
     3901 caggtgtttt ttcagtatca ggtccaacca aattcgcgcg cttctgccat tgccctccat
     3961 acaggggtgg gcaacattca tttccacata ttt
//
LOCUS       DAORLF010000437         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12450_length_3961_cov_1.941116, whole genome shotgun sequence.
ACCESSION   DAORLF010000437 DAORLF010000000
VERSION     DAORLF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12450_length_3961_cov_1.941116"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PK198_23390"
     CDS             complement(<1..912)
                     /locus_tag="PK198_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF41766.1"
                     /translation="MEKKIQLLQQKTAEAMLGGGQDKIDAQHQKGKLSARERLHFLLD
                     PGSFEEIGMLVTHRSSDFGMENQIIPGDGVITGYGTIHGRLVYVFSQDFTVFGGSLSE
                     THAEKICKIMDMAMKNGAPVIGLNDSGGARIQEGVNSLGGYADIFYRNVQASGVVPQI
                     SAIMGPCAGGAVYSPAITDFILMVEQTSYMFVTGPNVVKTVTNEEVSSEDLGGAMAHA
                     AKSGVTHFTAGNGLECIALIKRLLSYLPQNCEDTPPALPYTPGDEERPQLQHLIPEDS
                     HQPYDMREVVAGIADEGSFLEVHKDYAE"
     assembly_gap    953..973
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<974..>1360)
                     /locus_tag="PK198_23395"
     CDS             complement(<974..>1360)
                     /locus_tag="PK198_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRF41767.1"
                     /translation="KVIKLDRETVTVMLEDKDEERRYVEVQAMDWEVLRYRIDPKDPE
                     KIESEVIGTFSQIPLRLAWAVTIHKAQGKTFDKVIIDMDKGAFEHGQTYVALSRCRTL
                     EGIVLRQALRPQDIRTDERVTEVYRL"
     assembly_gap    1361..1372
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1373..>1715)
                     /locus_tag="PK198_23400"
     CDS             complement(<1373..>1715)
                     /locus_tag="PK198_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41768.1"
                     /translation="SKVYRQEQRHFLRLLDAVRLNEIDYEVLEELNARCEPDFQAQDF
                     YITLSSRNAKADAINSAELAKIPLPEERYIAAVTGEFNPSQYPAEPALRLKLNAQVMF
                     LKNDPDRRFVNG"
     assembly_gap    1716..1762
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1763..>2066)
                     /locus_tag="PK198_23405"
     CDS             complement(<1763..>2066)
                     /locus_tag="PK198_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41769.1"
                     /translation="GFPPSMCPESEVKKRRDRRLYTQLEVLVIDEISMVRADLIDHID
                     LFMRINREDPRPFGGLQVVFFGDLFQLPPVLSSDAEATFFRQQYATPYFFSAKVFRR"
     assembly_gap    2067..2125
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2363..3511
                     /locus_tag="PK198_23410"
     CDS             2363..3511
                     /locus_tag="PK198_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41770.1"
                     /translation="MKRAHTLLIFAALAALLHSCAPPVKLLIDGQYEKAYRNTISQLR
                     NGLPANESRRLVLEASYLQLFEAETAKEQALLNHDDEDRWIDLAEHYRQMKKRRAEAQ
                     RWLPTDIGYSMERLDLLHADAARRAHDFCVAEAENRADEARSGDKSAARQAFNLLQIG
                     GGYAALSSELQGFRQEMQRAGTYTLWVNYGADDATQRWTQRLHRGFYAPKTYNWLEIL
                     HEQPQDRPADFRLDAVLERAYASPESQSVSSRTVCKEIQDGEDIVRETVKVNDSTTVQ
                     VEKRIPRMITVTATIYQTTQTKEADVLMRYRLYRQADNALLDEWVLNSSDSFCNTYES
                     FSGDSRALDACCSGMSHITPSDHDMIQNAVAGLRRQLDRYLTREFRWP"
     gene            complement(3600..>3961)
                     /locus_tag="PK198_23415"
     CDS             complement(3600..>3961)
                     /locus_tag="PK198_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41771.1"
                     /translation="QTNLRGEVDAFVFLGIQDGVKDIVFKREAEAVAIAKNELEQYVG
                     EYELGGMVIKAYLRGEVLMVLVPGQPDYEMLPTGNHTFKFKALEGYSVRFETEADGKV
                     GAMNFIQPNGTFRAARK"
BASE COUNT          792 a         1036 c         1108 g          886 t
ORIGIN      
        1 ttcggcgtag tctttgtgta cctccaaaaa cgaaccttca tcggcaatgc cggccaccac
       61 ttcgcgcatg tcgtagggct ggtgcgagtc ttcggggatg aggtgctgca gttggggccg
      121 ctcctcatcg ccgggcgtat agggcagggc aggcggcgtg tcttcgcagt tctgcggcag
      181 gtagctgagc agccttttga tgagggcgat gcactccagc ccgttgccgg cggtgaaatg
      241 ggtgacgccc gatttggccg cgtgggccat ggcgccgccg aggtcttcgg aactgacctc
      301 ctcgttggtc acggttttga ccacattggg gccggtgacg aacatgtagg aggtttgctc
      361 caccatgagg atgaagtcgg tgatggcggg cgaatacacc gcgccgcccg cgcagggacc
      421 catgatggcg gagatttggg gcaccacgcc ggaggcctgc acgttgcggt agaaaatatc
      481 ggcatagccg ccgagggagt tgacgccctc ctgaatgcgg gcgccgccgg agtcgttgag
      541 gccgatgacg ggcgcgccgt ttttcatggc catgtccatg attttgcaga ttttttcggc
      601 atgggtttcc gacagggagc cgccgaacac ggtgaaatcc tgcgagaaga catagaccaa
      661 tcgcccgtgt atggtgccgt atccggtgat gacgccgtcg ccgggaatga tctggttttc
      721 catgccgaaa tcgctggagc ggtgcgtgac gagcatgccg atttcttcaa aggagcctgg
      781 atcgagcagg aaatgcaggc gttcgcgggc gctgagtttg cctttctggt gctgcgcgtc
      841 tatcttgtct tgcccgccgc cgagcatggc ttctgccgtt ttttgctgaa gcagttgtat
      901 ttttttttcc attggttgtg tgttggtctt gcgtttatag tggagcaaag atnnnnnnnn
      961 nnnnnnnnnn nnnatcaacc tgtaaacctc cgtcacccgc tcgtcggtgc ggatgtcctg
     1021 cgggcgcagg gcctggcgca gcacgatgcc ctccaaagtg cggcagcgac tgagggccac
     1081 gtatgtctgc ccgtgctcga aagcgccttt gtccatatca atgatgacct tgtcgaaggt
     1141 tttgccctgc gctttgtgaa tggtgacggc ccaggccaat cgcagcggta tctgactgaa
     1201 agtgcctatg acctcgcttt caattttttc cgggtctttg gggtctatcc ggtagcgcag
     1261 cacttcccag tccatggcct gcacctccac atagcggcgc tcctcgtcct tgtcttccag
     1321 catgacggtg acggtttcgc ggtccaattt gatgaccttg nnnnnnnnnn nngccgttga
     1381 cgaagcggcg gtcggggtcg tttttcaaaa acatgacctg cgcgttgagc ttgaggcgca
     1441 aggccggttc tgccggatat tgcgaggggt tgaactcgcc ggtgacggca gcgatgtagc
     1501 gctcttcggg caggggtatt ttggccaatt ctgccgagtt aatggcgtcg gctttggcgt
     1561 ttcggctgga gagggtgatg tagaaatcct gcgcctgaaa atcgggttcg cagcgggcgt
     1621 tgagttcttc aagtacctca taatcaatct cattgaggcg cactgcatca agcaatctca
     1681 aaaaatggcg ctgctcctgc cgatagactt tgctcnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nncccggcgg aaaactttgg cagaaaaaaa gtaaggcgtg
     1801 gcgtattgtt gtctgaaaaa agtggcttcg gcatcggaag acagcacggg cggaagctga
     1861 aacagatcgc cgaaaaacac tacctgcaat ccgccgaaag gccgcgggtc ttcccggttg
     1921 atgcgcataa acaggtcaat gtggtctatc agatcggcgc gcaccataga aatttcgtct
     1981 atgaccagca cttcgagttg ggtgtagagc cgtcggtcgc gccgcttttt gacctcgctt
     2041 tcggggcaca tactcggcgg gaaaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnaggcg ccagcacgac cgtttttttt cgggtggtac
     2161 ttctgaaaag ctgcaacagc gtggacttgc cggtgcccgc ccgcccggtg atgaacaggt
     2221 gcagcccctt gttttcaatg gcatccagcg cgtattgaaa atcggcgttg agttcgagcg
     2281 gttgtttgtc cagcatttaa cttcttctta aaaccattgc atctgcaaag ctgctatttt
     2341 tggcaaataa aaccaaacag tcatgaaacg cgctcacacc ctgttgatct ttgccgcact
     2401 ggcagccctg ttgcacagtt gcgcgccgcc cgtcaaattg ctcatagacg gccaatacga
     2461 aaaagcttat cgcaacacca tcagccaatt gcgcaacggg cttccggcca atgaatcgcg
     2521 gcgattggtg ctggaagcct cgtatttgca actgtttgag gccgagaccg ccaaagaaca
     2581 ggccctgctg aaccacgacg acgaagaccg ctggatagac ctggccgagc actatcgcca
     2641 gatgaaaaaa cggcgggcag aggcgcagcg ctggctaccg accgacatag gctactcgat
     2701 ggagcggctc gatttgttac atgccgatgc ggcccgtcgt gcacacgatt tctgcgtggc
     2761 cgaagcggaa aacagggctg atgaagctcg ttcgggagac aaaagcgctg cccggcaggc
     2821 gttcaatctg ctgcaaattg gaggcggata cgcagcttta tcgtctgaat tacaaggctt
     2881 tcggcaagag atgcagcgcg ccggcactta tacgctttgg gtaaactatg gcgccgatga
     2941 tgctacccaa cgttggacac agcgcctaca ccggggtttt tatgcaccca agacttacaa
     3001 ctggctcgaa atcctgcacg aacaaccaca agaccgcccg gcagactttc gcttagatgc
     3061 cgtgttggaa cgcgcttacg cttcgccgga atcgcaatcg gtgtcgagcc gcactgtttg
     3121 taaagaaatt caagacggcg aagacattgt ccgggaaacc gtaaaagtaa acgactcgac
     3181 tacggtgcag gtggaaaaac gcatcccgcg catgattacc gtcaccgcta ccatctatca
     3241 aaccactcaa accaaagagg ccgatgtgtt gatgcgctac cggctgtacc gtcaggcaga
     3301 caacgcgctg ctcgacgagt gggtgctgaa cagcagcgat tcgttttgca atacttatga
     3361 atcgttctcc ggcgacagtc gcgcattaga cgcctgttgc tcgggcatgt ctcatattac
     3421 gccttccgac catgacatga ttcaaaatgc ggtggcgggt ttgcggcggc agttggaccg
     3481 gtatctgacg cgggagtttc ggtggccctg atggtaggct acgcttagtg cgccaccgga
     3541 tgaggtttgg tttccaccat tgtcagtccg ttttggattg cttcgccttc ggcaaatgtt
     3601 tacttcctcg ccgcccggaa agtcccgttg ggttggataa aattcatggc gccgaccttg
     3661 ccgtctgctt cggtctcaaa ccggacgctg tagccttcta atgccttgaa tttgaaggtg
     3721 tgattgcccg taggcagcat ttcataatcg ggttggccag gcaccaatac catcagtacc
     3781 tcgccgcgca ggtaggcttt gatgaccata ccgcccaact cgtattcgcc cacatattgc
     3841 tccaattcgt tcttcgcaat ggccacagcc tcggcttccc gtttgaacac gatgtctttc
     3901 accccatcct gaatgccgag gaagacaaaa gcgtccactt cgccgcgcag attggtctga
     3961 a
//
LOCUS       DAORLF010000438         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12481_length_3953_cov_1.986916, whole genome shotgun sequence.
ACCESSION   DAORLF010000438 DAORLF010000000
VERSION     DAORLF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12481_length_3953_cov_1.986916"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PK198_23420"
     CDS             <1..1126
                     /locus_tag="PK198_23420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41772.1"
                     /translation="DIWTQVFGAQSGRVKRVLGTQAVNPWISQQMMAYIKDGFDFLSP
                     TWYFGYSGSACEGSIVTPQDVLDCSISNWRTAASAFRQQYRDAKMYGKGVVEYEGGQH
                     MTSNPATVPFQQAVYDAQIDPNIALLYNEVLDSLRRWGSSLAMAFTLASVRESIYGSW
                     GALEDINHDGSNPPAPKWSVLNSNIHCYTPPVTVNPVLAVEWVAFEGREDGCSVVLNW
                     RTGAETNASHFEVQRSFDGRHFTTIGRVAARGQASAYSFTDTEIGSTKAYYRIAENDF
                     DGRKSYSVIRHLSGRCPSVFSLHPNPAGDFITLTLSENMPHSTREVWLFDAQGRLLRK
                     EVWPSGDRHELSIEDLPAGLYWLGLYDGRGHVSRQRLVKF"
     gene            1208..>1392
                     /locus_tag="PK198_23425"
     CDS             1208..>1392
                     /locus_tag="PK198_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41773.1"
                     /translation="MPAVEIRKGVQVGLDDLVSSLSSLETGDLKLVSDRIHQLLSSRS
                     DYAETEREKELLQLIKE"
     assembly_gap    1393..1483
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1615..>1819
                     /locus_tag="PK198_23430"
     CDS             1615..>1819
                     /locus_tag="PK198_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41774.1"
                     /translation="MRLHLPSITRYTVAVAALFCIAAAMAGAVAGHHKAIQFIAHLAG
                     QSDKAHKIEAAFPPGKFWALLGIL"
     assembly_gap    1820..1833
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1834..>2198
                     /locus_tag="PK198_23435"
     CDS             <1834..>2198
                     /locus_tag="PK198_23435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41775.1"
                     /translation="GIAFRFVNIWLPYPAAGLRYALQSTRQTIVSFAASPAKYLLILP
                     VAASVYYALTLPVNYDEAWTYLNFTSKSPIVSLAYYPVPNNHILHSLITNFTRYLPGF
                     SVLTALRISSIFVNIGIWV"
     assembly_gap    2199..2395
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2396..3299
                     /locus_tag="PK198_23440"
     CDS             <2396..3299
                     /locus_tag="PK198_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41776.1"
                     /translation="RLRHWAMWAVCSIGGFFTMPSFLYPVLALNAWIFWQQPGGWRRQ
                     MYANLATAVTVAILYLPAVLFSGIKALAANPFVQPISRSEVFTRLPEFFAGALEDVFG
                     MPFYIPAALMLGSLLLAALRRDRFHTKMGIVFALAPWLLLLLHSVIPFSRTFAWYALV
                     IPVMALIPWAAHLQRVPSSVLMSILLIVQVWSLWNFHQKIGEFEQYNTTYHKINQEIA
                     GPHSYYFNTRTFDTNLLFELRIRGFEPTKQQRNFPPLYMSADSISGFDYIIIDKPFDL
                     TKNRKPTFSDPYVSIYSSENAGYE"
     gene            3483..>3953
                     /locus_tag="PK198_23445"
     CDS             3483..>3953
                     /locus_tag="PK198_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41777.1"
                     /translation="MNTGRITLICLCLAIPLILTGQQNYTIGFHSGNAPDAAYLKPAT
                     VTPYMLEETAFNGRHYLLLQFEDVPDNAQLQMLSALDVQLLHYIPDYAWYASLPESMP
                     VPDLPPFVKSVLIPHPIWKLSPELARQEYPPHALSSAGIRVQVLPYDDIAPAMLA"
BASE COUNT          840 a         1014 c          895 g          902 t
ORIGIN      
        1 cgacatctgg acgcaggtgt tcggcgctca gagcggccgt gtgaagcgcg tactcggcac
       61 gcaggccgtc aatccctgga tcagccagca aatgatggcc tacatcaaag acggcttcga
      121 tttcctatcg cccacctggt acttcggcta ctccggcagc gcctgcgaag gctctatcgt
      181 cacaccgcaa gacgtgctgg attgcagcat cagcaactgg agaactgccg cttccgcctt
      241 ccggcaacag taccgggatg ccaaaatgta cggcaaaggc gtggtggaat acgaaggcgg
      301 ccaacacatg accagcaatc cggctaccgt gcccttccag caagccgttt atgatgccca
      361 aattgatccc aacattgccc tgctgtacaa cgaagtattg gacagcctgc gccgctgggg
      421 ttcctcattg gcaatggcgt tcacactggc ttctgttcga gaatccatct atggctcatg
      481 gggcgcactg gaagacatca accacgacgg cagcaatccg cccgcgccca agtggagcgt
      541 tctgaacagc aacatacatt gctacacgcc gccggtgacg gtgaaccccg tgctggcggt
      601 agaatgggtg gcttttgaag gccgggaaga tggttgttcg gtggtattga actggcgaac
      661 aggcgcagaa accaacgctt cccactttga ggtacagcgc agtttcgacg ggcggcattt
      721 cacgaccatc gggcgggtag cggctcgtgg tcaggcctcc gcatactcgt ttaccgacac
      781 ggagatcggc tcgacaaaag cgtattaccg catagcggaa aatgattttg acggtcggaa
      841 atcctattcc gttatccgcc atctgagcgg acgctgtcct tctgttttca gcctgcaccc
      901 caatcccgcc ggcgatttca tcaccttgac tttatcggaa aatatgccgc acagcacccg
      961 cgaggtatgg ctgttcgatg cacagggtcg cttgctgcgc aaggaagtat ggccttccgg
     1021 agatcggcat gagttgagca ttgaggactt gcccgccgga ctgtactggc tggggcttta
     1081 tgacgggcgg gggcatgtca gcagacaacg attggtgaaa ttttaaaaaa aatgccaatg
     1141 gcaggttgcc gcaagaatct tgcatatatc gttcatatcc attatttttg cacaaaacat
     1201 agacaatatg ccggcagtag aaattcgcaa aggagttcaa gtagggttag acgacttggt
     1261 gagcagttta tcttcattgg aaacaggaga cctgaaattg gtatcagatc gcatccatca
     1321 attgctttcg tccagaagcg actacgctga aactgagcgg gaaaaggaac ttctccaact
     1381 gataaaggaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatgctt atgcttactg
     1501 attttatgga atccaaaagt gccgagcggc tttatttgat gggcgaattg gctcaaatga
     1561 agcgctgaaa ttagtagggg ttcatcctgc tatggtgagc aaataatttc cctcatgcgc
     1621 ctacatcttc cgtccatcac ccgctatacc gttgccgtcg cagcattgtt ctgcattgcc
     1681 gcggccatgg ccggcgctgt ggctggtcat cacaaggcca ttcagttcat tgctcatctg
     1741 gccgggcaga gcgacaaggc ccacaaaatt gaggcagcct ttccgcccgg caagttttgg
     1801 gcgctgctcg gtattctgcn nnnnnnnnnn nnntcggcat cgcttttcgt tttgtcaata
     1861 tctggctccc ctaccctgcc gccggcctgc gctacgcgct ccagagcacc aggcaaacca
     1921 ttgtttcatt tgctgcctcg cccgccaaat acctgctgat attgcccgtg gctgcctccg
     1981 tttattatgc cctcaccctg ccggtcaact acgatgaagc ctggacctac ctcaatttca
     2041 cctccaaatc gcccatcgtt tcattggctt actacccggt gcccaacaac catattttgc
     2101 actccctcat caccaatttc acccgttacc tgcccggctt cagcgtgctg acggcgctgc
     2161 gcatttcctc tattttcgtt aacatcggca tttgggtann nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggc
     2401 tgcggcactg ggcgatgtgg gccgtgtgca gtatcggcgg ctttttcacc atgccctcgt
     2461 ttttgtatcc ggtcttggcg ctcaacgcat ggattttttg gcagcagccc ggcggatggc
     2521 ggcgtcagat gtatgccaac ctcgccaccg cagtgacggt ggccattctg tatttgccgg
     2581 cagtcctgtt cagcggcatt aaagcgctgg cggccaatcc attcgtgcag cccatcagcc
     2641 gcagcgaggt atttacacgg ctgcctgagt tttttgccgg agcattggag gatgtattcg
     2701 gaatgccgtt ttacataccg gcggctctga tgctgggcag tctgttgttg gcggcgctcc
     2761 ggcgcgaccg ttttcatacc aaaatgggca tcgtttttgc gctcgccccc tggctattgc
     2821 tattgttgca ctccgtcata cccttcagcc gcacctttgc ctggtatgct ttagtgattc
     2881 cggtgatggc actgataccc tgggcagcac atttgcagcg tgtgccaagc tccgtgttga
     2941 tgagtatttt gctgatcgtt caagtctggt cattgtggaa ttttcatcaa aaaatcggcg
     3001 agttcgagca gtacaacacc acataccaca agatcaatca ggaaattgcc gggccgcata
     3061 gctattactt caacacccgc acttttgata ccaatttgct gtttgaattg cgcatccggg
     3121 gttttgaacc aacgaaacag caacgcaatt tccctccgct gtacatgagt gccgacagca
     3181 tttccggctt cgactacatc atcattgata aaccctttga tcttaccaag aaccgaaagc
     3241 caacattcag cgatccgtac gtcagtatct atagctccga aaatgctggt tatgaataac
     3301 ttacaatttt acaaatgtta acaaaccaca tttttatgta ataaattatg atttataaaa
     3361 aataagcata tatatttgca ctacttatac aggaacgact ttatcctgac caatccgcca
     3421 atacaacaca tactgatttt ttttcgagca gctgggaggc caaaaacagc aatcacacac
     3481 ttatgaacac tggcagaatc acactgattt gcctttgtct ggccatccct ttgatcctga
     3541 cagggcaaca aaactacaca atcggcttcc acagcggcaa cgcgccggat gcggcttatc
     3601 tcaagcccgc taccgtcact ccgtatatgc tggaggaaac agcctttaac gggcgccact
     3661 acctgttgtt acagtttgaa gacgtacccg acaacgctca actacagatg ctgagcgcat
     3721 tggacgtgca attgctgcac tatatacccg actatgcctg gtatgcgagt ttgcccgaat
     3781 ctatgccggt accagacctg cccccatttg taaaatccgt attgatacct catcccatct
     3841 ggaagttatc gccggaactg gcccggcagg aatatcctcc gcacgccttg agcagcgccg
     3901 gaatcagggt acaggttttg ccttatgacg acatagcgcc cgccatgctg gcc
//
LOCUS       DAORLF010000439         3931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12569_length_3931_cov_2.102941, whole genome shotgun sequence.
ACCESSION   DAORLF010000439 DAORLF010000000
VERSION     DAORLF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12569_length_3931_cov_2.102941"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /gene="dnaE"
                     /locus_tag="PK198_23450"
     CDS             <1..1815
                     /gene="dnaE"
                     /locus_tag="PK198_23450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRF41778.1"
                     /translation="SAGLLKMDFLGLKTLSIIKDAVRMVKENHGIDIDPEEVPLEDSK
                     TYELFKRGETVAIFQYESAGMQKYMKELAPTTFEDLIAMNALYRPGPLEYIPEFIDRK
                     HGRKPVVYDLPAMEQYLSDTQGITVYQEQVMLLSQKLAGFTKGQADMLRKAMGKKQKA
                     VLDSMYPKFLEGGSGNGHPQDRLEKIWKDWEAFASYAFNKSHSTCYAFVAFQTAYLKA
                     HYPGEFMASVLTHNKSNLEQVTFFLSECRRMNIQVLGPDVNESVSDFSVNKEGQIRFG
                     LSALKGVGEGPVEAILEERRKNGHFSSIFDLMQRLPLQALNKRVMESLAFGGAFDCFS
                     DLHRAQYFTPSEKYDSFIEHLLKYGSAYQGEKAMAASSLFGETGEVMIPPPAPPQCEP
                     WSLLEKLDKEKDVTGIFISGHPLDDYRIEIENFITCTLDHISEHQQKPLLQVAGMVTR
                     ATHKISKNGNGWGIFEIRDYNAGIEFKLFGEDYQKFKHLLEDGKALFIKGAFQKGWNS
                     EELEFKPKEIKLLEGVGADLTQSITLKLPLERLDMELIDRLDAACQRHKGPHRLRLEL
                     TDSAQRLRVQTYARERKVLAAAAFIEELDKMQLEYRLN"
     assembly_gap    1835..1902
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1903..2717)
                     /locus_tag="PK198_23455"
     CDS             complement(<1903..2717)
                     /locus_tag="PK198_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41779.1"
                     /translation="MSQFHWSYYSTTGQPYIVGLYHGPESGHVVVYCNNEVIAIDFGV
                     KESGLYSFFIEEDLCEIRIQRKRRKFKYSFESNQKVDTPFNRMRRKWLRLHKMQRTFA
                     AVVVALFAIGMAVTMLNQWQRNRHTEDNSVLYQGYRTSARLRVEPDAEGKQYAVFSYA
                     AEGRVREGRVPLPPDLVTGNGLPLENGDEFAVYYLPHRPKVYAVQWNEPVPELLDQYR
                     QRVLQRLTTLQPGLSGAAAECLLDVTYEIAGLSGWADLWWQQAPPEQNARHNSR"
     gene            2904..3506
                     /locus_tag="PK198_23460"
     CDS             2904..3506
                     /locus_tag="PK198_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HRF41780.1"
                     /translation="MFYTFKGFRPVVHPSAFVHPQAAVTGNVIIGRDVYIGPGAAIRG
                     DWGGIVIEDGCNVQENCTVHMFPGITVYLREGAHIGHGAVIHGAQVGRNVLVGMNAVV
                     MDEVEIGEGSIIGALCFVKAGEKIPPRSLVAGNPGRIIKEVSDDMLAWKTQGTRLYQT
                     LPDECRTSWEPCEPLREMPADRPAQESLYDTWRRVKEGGT"
     gene            3676..>3931
                     /locus_tag="PK198_23465"
     CDS             3676..>3931
                     /locus_tag="PK198_23465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRF41781.1"
                     /translation="MILLAKFDWTLPLKSPVLIFSLILFIILFAPIILNRFRIPHLIG
                     LIIAGALIGPNGLNLMLRDSSIVLFGTVGLLYIMFLAGLEI"
BASE COUNT          922 a         1045 c         1028 g          868 t
ORIGIN      
        1 tcggcgggcc tcctcaaaat ggactttttg ggcctcaaaa ccctctccat catcaaagac
       61 gccgtgcgca tggtgaagga aaatcacggc atagacattg accccgaaga agtgccgtta
      121 gaagactcca aaacttacga gctttttaag cgcggcgaaa cggtagccat tttccagtac
      181 gaaagcgccg gtatgcagaa gtacatgaag gaactggcgc ccactacttt tgaagacctc
      241 attgccatga acgccctgta ccggcccggc ccgctggaat acattccgga gttcattgat
      301 cgcaaacacg gcaggaaacc agtggtgtac gatcttccgg caatggagca atatttatct
      361 gatacacagg gtattaccgt ctatcaggaa caggttatgc tcctttctca gaagctcgcc
      421 ggcttcacca aaggccaggc cgacatgttg cgcaaagcga tgggcaagaa gcaaaaagcc
      481 gtgctggact ccatgtatcc caagtttttg gagggcggtt cgggcaacgg ccaccctcag
      541 gaccggctcg aaaaaatctg gaaagactgg gaagcattcg cctcttacgc attcaataaa
      601 tcgcactcta cctgctacgc tttcgtggcc ttccagacgg cctacctcaa ggcgcactat
      661 ccgggggagt tcatggcctc ggtgctcacc cacaacaaga gcaacctcga acaggttact
      721 tttttcctca gcgagtgccg ccgcatgaac attcaggtgc tcggcccgga tgtgaacgag
      781 agcgtatccg acttttcagt gaataaagaa ggccaaatcc gcttcggtct ctccgcgctc
      841 aaaggcgtgg gcgaaggccc ggtggaagcc atcctcgaag agcgccgcaa aaacgggcac
      901 ttcagcagca tcttcgacct catgcagcgg ctgcccctgc aagcgctcaa caagcgcgtg
      961 atggaaagcc tggcattcgg cggggcattc gattgcttct ccgatctgca tcgcgcacag
     1021 tattttacgc cgtctgaaaa gtacgattct ttcatcgaac acctgctcaa gtacggcagc
     1081 gcatatcagg gcgagaaggc gatggcagcc agttcgctct ttggcgaaac cggcgaagtg
     1141 atgattccgc ctcctgcgcc gccgcagtgc gaaccctggt ctttgttgga aaaattagac
     1201 aaagaaaagg acgtgaccgg cattttcatc agcggccacc ccttagatga ctaccgcatc
     1261 gagatcgaaa atttcatcac ctgtacgctc gatcacatca gcgaacacca gcaaaaaccg
     1321 ctgctgcaag tggccggcat ggtcactcgc gccacgcaca agatcagcaa aaacggcaac
     1381 ggctggggta tttttgaaat acgcgactac aacgccggca tagagttcaa gctctttggc
     1441 gaggattatc aaaaattcaa acacctgctc gaagacggca aagcgctgtt catcaaaggc
     1501 gcattccaaa agggctggaa ctcggaggaa ttggagttca aacccaaaga aatcaaactc
     1561 ttggaaggcg tgggcgccga tctcacacag tccatcaccc tcaaactgcc tctcgaacgc
     1621 ttagatatgg aactgataga ccgcttggat gctgcctgcc aacgccacaa aggcccgcac
     1681 cgcctgcgtt tggaactgac agactctgcc cagcgcctgc gcgtacaaac ctacgccagg
     1741 gagcgaaaag tgctggccgc cgccgccttc attgaggaat tggataagat gcagttggag
     1801 tataggttaa attgagcgtg ctactccgcg caccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggctgtt gtggcgggcg
     1921 ttttgttctg gaggcgcttg ttgccaccac aaatcggccc agcccgaaag ccctgcaatt
     1981 tcgtatgtca catccagcag gcactctgct gcggcgcccg acaggccagg ctgcaaggtc
     2041 gtcaggcgtt gtaaaacccg ctgccggtat tggtccagca gttcgggtac aggctcgttc
     2101 cattgcacgg catatacttt ggggcgatgg ggaagataat agactgcaaa ctcgtcgccg
     2161 ttttccagcg gcaggccgtt gcccgtgacg agatcgggcg gaagcggcac gcgcccttcc
     2221 cgaacccgcc cctcggcagc ataagaaaat actgcatact gctttccctc ggcatccggc
     2281 tccacgcgaa gacgggcgga ggttctgtaa ccctggtaca acacgctgtt gtcttcggta
     2341 tgccggttgc gttgccactg gttcagcatc gtgacggcca ttcctatggc aaagagtgcg
     2401 actaccactg cggcaaaagt tctttgcatt ttgtgcaatc gcagccattt gcgccgcatt
     2461 cgattgaagg gcgtatccac tttttgattg ctctcaaagc tgtatttgaa cttgcgccgt
     2521 ttgcgctgaa tgcgaatttc gcagaggtct tcttcaataa aaaaagagta caagcccgat
     2581 tctttgacac caaaatcaat ggcaatcacc tcgttgttgc aatacaccac cacatgcccg
     2641 ctctccggcc cgtgatacaa cccgacgatg tacggctgcc ctgttgtgct gtaatatgac
     2701 cagtggaatt gcgacaatgg aaagcttttt gttgataact taaacgaata tgtatagctg
     2761 tgagccacga gccgccttat gagaagtgtc ggcagttctt gagtaacgca atacctccgc
     2821 gaaagataca aattttcaaa tcggaacact acttttgcag gcgtctgttg ctcctgtgta
     2881 gtggggcaaa aaacaatagt gccatgtttt acactttcaa aggattccgg cccgtcgtac
     2941 acccctctgc gtttgtacac ccgcaggccg ccgttaccgg caacgtaatc ataggccgcg
     3001 atgtttacat tgggcctggc gcggccattc ggggcgactg gggcggcatc gtcatagaag
     3061 acggctgcaa tgtgcaggaa aactgtaccg tccacatgtt tcccggcata accgtttatt
     3121 tacgagaggg cgcgcacatc gggcacggcg cggttataca cggtgcgcag gtaggccgca
     3181 atgtgttggt gggtatgaat gccgtggtga tggatgaggt agaaataggc gaagggagca
     3241 tcatcggggc cttgtgtttc gtcaaagccg gcgaaaaaat tcctccgcgc agcttagtag
     3301 ccggcaatcc ggggcgcatc atcaaagagg tgagcgatga tatgctggca tggaaaacgc
     3361 agggcacccg gctgtatcag accttgcccg atgagtgccg aacctcttgg gaaccttgcg
     3421 aacccttgcg ggaaatgcct gccgaccggc cggcgcaaga gagcctgtac gatacctgga
     3481 ggagagtgaa ggagggggga acctgattgc ttggcattat cggtaagctg caagccgtca
     3541 gctcgttttt tagttcagga atcagatgtt cctcttgggg gaatttctaa gtttctatga
     3601 gtagtcaagg ggtgactacg ggttgtactc ttggctgaat caccccttca ctacgaggcc
     3661 gaaaatatga tttttatgat cttacttgcc aaattcgact ggacgttgcc gctgaaaagc
     3721 ccggtgctga tattttcgct gattctgttc atcattttgt ttgcgcccat tattttgaac
     3781 aggtttcgca ttccgcacct catcgggctg atcatcgccg gcgccctcat cgggccaaac
     3841 ggcctcaatc tcatgttgcg cgacagcagc atcgtccttt tcggcaccgt gggtttgttg
     3901 tacatcatgt ttttggccgg tttggaaata g
//
LOCUS       DAORLF010000440         3929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12581_length_3929_cov_1.941662, whole genome shotgun sequence.
ACCESSION   DAORLF010000440 DAORLF010000000
VERSION     DAORLF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_3929_cov_1.941662"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            962..3034
                     /locus_tag="PK198_23470"
     CDS             962..3034
                     /locus_tag="PK198_23470"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HRF41782.1"
                     /translation="MESPQAVRDYFEELGEDGRKLNEVKVVFLGEASAGKTSLVKQLM
                     GEQFDDKESQTHGIRIRKMDFGMSDGDTVTAHLWDFGGQEVMHATHQFFLSQRSVYVL
                     LLNSRNDDQAEKWLKYAESFGGRSPVLVVLNKIDENPSFEVNRKTLREKYPQIREFYR
                     LSCQNKSGIDDFREALRREIENADTRRTPFPAHWLAVKEHFSNMQADYIESEEFRREC
                     EKAGVVREFSQDVLLKFLHDLGVVINFRNLKNFDTQILNPLWLTNGVYRIINSGRVAE
                     TGGLLHEDEFDTVINDPRYRAENTTDKPFHYPKNKLQYIVRVMQEFELCFQLDPWTYV
                     VPQLLPVQEPDVPMEGAVLRFVIHFPDFLPDSLFPRLMVKLHTYIEDKEVLGETFSDR
                     VTRSHPVTKPAHSSHTKLAWRTGMVLHKPNIFKASARVRWDKEDRKILVDVCGEERRR
                     FLSFIRETVKEIVATFSSLSYSELVPIPDSTDTEEYEYLVEAEKAGEKEVFVKSLKKR
                     VSIADLLDGVEEPAMRDEVEQLPVKAFVSYAHKDLEYLKEMRSALAPLIRLQKLQLWH
                     DRDISAGDEWREVLFQRLDEADIVLCLVSADFVASDFCYQKEFGAALEAHRRGEKTIV
                     PVKLRKTDWQDLPIAEFQGVPGEWITSAQNKDEAWAQVSASLRPALDKAKQRKSKVLE
                     ERRMGGGR"
     gene            complement(3049..>3929)
                     /locus_tag="PK198_23475"
     CDS             complement(3049..>3929)
                     /locus_tag="PK198_23475"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF41783.1"
                     /translation="QACPGSPVDYNGNMLTPGTTTDFTLTNAAGCDSIVTVTVTAYPS
                     MLVQTMSEESCPGQPTGSISVSATGQGPFRYRINGGEWQNGAVFSNLRAGTFHLLTED
                     GNGCSAETRVVLEELAALQLVVPDAVLPCNGEPVMIEALVLSGHDQSMQLQWDDGSSS
                     AQRMVTEPGAYRITAINGCDTLTQTLEVRYADTPDLAPVYIPNAFSPNDDGVNDYFRG
                     YASDEVSVQDYDLMIFDRWGNLLFHTRRMEEGWDGQFRGRKMEPGVLVWHLRATVLHC
                     GRTLQLNEKGDVTLIR"
BASE COUNT          974 a         1101 c         1009 g          845 t
ORIGIN      
        1 cctcgctcgc cggctcatcc gcgaaaacat tgaaaaacac caacggggag aagatgcccg
       61 cgcgctcgac ctcggcaact gcggcatgac cgaaataccg gaggagattg gggagtgcgt
      121 gtggttggag gagttgaggc tgggtcatta ctggcaatat ttcaaccaca acatatacga
      181 ttggagaata cgagcaacta taaatagagg tggggctaat aaaattatca aactcccttc
      241 aacaattaaa cggctcttga atctgaggaa aatctcgatt acacaattgc cgatcacaga
      301 tattcaacca ctatcagcct tggtgaattt acagtggatt gattgctcac aaacacaaat
      361 ttctgatctt agtccgttat cacaactatt acaattacag ttacttgact gctctggaac
      421 aagtgtttac gatctaagcc ctctatctaa attaagtaac ctacaaatac ttagtaccca
      481 cttaacaaat gtatcggatt tatacccctt atctacattg accaatttac aatcacttca
      541 tatctccgac actcccatat cggaaatcga cccaattatg tctcttagga atttacgatc
      601 actattctta gcctatacaa acatcttgga gattagttgt ttggaaaaac tatccaaatt
      661 gcaatggttt gattgctctg gcttgcaaat atcaaatatt gacgttttat caaaactaac
      721 cagtttgaaa tgggttgact gctcttatac aatcgtttct aaccttgccc ctttatcaaa
      781 attggagcaa ttacagtggc ttgattgttc cggaactgaa gtttctgacc ttactccagt
      841 tttgccctta attcataaag gaattgaagt taaatggcag gatcaataca caggacatca
      901 agagcacccc gccatcctcg tccgcgactg ccccctcatc atcccccccg tcgaattcgc
      961 catggaatcc ccccaggccg tgcgcgacta cttcgaagaa ctcggcgaag acgggcgcaa
     1021 gctcaacgag gtcaaggtcg tttttctcgg cgaggcatcg gcggggaaaa cctctttggt
     1081 gaagcagctc atgggcgagc aattcgacga caaagagagc cagacccacg gcatccgcat
     1141 ccgaaaaatg gacttcggca tgagcgacgg cgacaccgtg accgcccacc tctgggactt
     1201 cggcgggcag gaggtgatgc acgccaccca ccagtttttc ctctcgcagc gcagcgtgta
     1261 tgtgctgctg ctcaacagcc gcaacgacga tcaggccgaa aaatggctca aatacgccga
     1321 gagcttcggc ggccgctcgc ccgtactcgt ggtgctcaac aaaatagacg aaaacccctc
     1381 cttcgaggtg aaccggaaaa ccctgcgcga aaagtatccg cagatccgcg agttctaccg
     1441 gctgtcttgc caaaacaaat caggcattga cgacttccgg gaggccctgc gccgcgaaat
     1501 agaaaatgcc gacacccgcc gcacgccctt ccccgcgcat tggctggccg tgaaggagca
     1561 tttttccaac atgcaggccg actacatcga atcggaggag tttcggcgcg agtgtgaaaa
     1621 agccggcgtg gtcagggagt tcagtcagga cgtgctgctc aaatttttgc acgacctcgg
     1681 cgtggtcatc aacttccgca acctcaaaaa cttcgacacc caaatcctca atccgctctg
     1741 gctcaccaac ggcgtgtatc gcatcatcaa ctccgggcgc gtggccgaaa ccggcggtct
     1801 gctgcacgaa gacgagttcg acaccgtcat caacgacccg cgctaccgcg ccgaaaacac
     1861 caccgacaag cccttccact atcccaaaaa caagctccag tacatcgtgc gggtgatgca
     1921 ggagttcgag ttgtgcttcc agttggaccc gtggacgtat gtggtacccc aactgctgcc
     1981 cgtgcaggaa cccgatgtgc cgatggaggg cgccgtgctg cgcttcgtca tccatttccc
     2041 cgacttcctg cccgacagcc tcttcccccg cctgatggtg aaactgcata cttacataga
     2101 ggataaggaa gtgcttggtg aaacgtttag tgaccgggtg actcgcagtc acccggtcac
     2161 taaacccgcc cactcatctc acaccaagct ggcctggcgc acgggtatgg tgctgcacaa
     2221 gcccaatatt ttcaaggcat cggcgcgggt gcgctgggat aaggaagacc gcaaaatcct
     2281 cgtggacgta tgcggcgagg agcggcggcg tttcctcagt ttcatccgcg aaacggtgaa
     2341 ggagatcgtg gcaaccttca gcagtctgtc gtacagcgaa ttagtgccga taccggacag
     2401 cacggatacg gaggaatatg aatacctcgt ggaagctgaa aaggcaggcg aaaaagaggt
     2461 atttgttaaa tccctcaaga agcgcgtttc catcgccgac ctgctcgacg gcgtggaaga
     2521 acccgccatg cgcgacgagg tggagcagtt gccggtgaag gcgtttgtgt cgtatgcgca
     2581 caaggatttg gagtacctca aagagatgcg cagcgccctg gccccgctca ttcgcctgca
     2641 aaaactgcaa ctctggcacg accgcgacat cagcgccggc gatgaatggc gggaggtttt
     2701 attccaacga ttggacgagg ccgacatcgt gttgtgtttg gtgagcgccg attttgtggc
     2761 ctccgatttt tgctaccaaa aagaattcgg cgcagcgctg gaagcccacc gtcgcggcga
     2821 aaaaaccatc gtacctgtga agctgcgcaa aaccgactgg caggatttgc cgatagccga
     2881 gtttcagggc gttcccggcg agtggatcac ctcggcccaa aacaaagacg aagcctgggc
     2941 gcaggtttcc gcatctttgc gcccggcttt ggacaaggcc aagcaacgca aaagcaaggt
     3001 gctggaggag cgccgcatgg gcggaggcag gtgagggctg ccgggctgct acctgattag
     3061 tgtcacatcg cccttctcgt tcaattgtag ggtgcgcccg cagtggagta ctgttgcacg
     3121 caaatgccag accagcacac ccggttccat tttccgacct ctgaattggc catcccagcc
     3181 ctcctccata cggcgggtat gaaaaaggag gttgccccag cggtcgaata tcatcaaatc
     3241 ataatcctgt accgatactt catctgaagc ataaccccgg aaatagtcat tgacgccgtc
     3301 atcgttcgga ctgaatgcgt tcgggatata aaccggcgcc agatcgggag tgtcggcata
     3361 acgcacttca agggtttgcg tcagcgtgtc gcagccgttg attgccgtga tccggtaggc
     3421 gccgggttcc gttaccatgc gttgagcaga agaagagccg tcgtcccatt gcagttgcat
     3481 actttgatcg tggccggaga gtacgagggc ttctatcatt accggctcgc cgttgcaggg
     3541 cagcacggca tccggaacaa caagctgaag cgccgccaac tcctccaaca caacgcgggt
     3601 ttccgccgag cagccgtttc cgtcctccgt caagaggtgg aaagtaccgg cacgcaggtt
     3661 ggaaaaaaca gctccatttt gccattcgcc gccatttatt ctgtaccgaa aaggcccctg
     3721 ccccgttgct gaaactgaaa tgcttcccgt aggctgcccc ggacaacttt cctcggacat
     3781 ggtttgcacc agcatcgaag gatatgccgt cacggttaca gtcacgatgg aatcgcaacc
     3841 cgccgcattc gtcagggtga aatccgtcgt agttccgggc gtgagcatgt taccgttgta
     3901 atcaaccgga ctgccgggac aggcttgca
//
LOCUS       DAORLF010000441         3880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12784_length_3880_cov_2.158431, whole genome shotgun sequence.
ACCESSION   DAORLF010000441 DAORLF010000000
VERSION     DAORLF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12784_length_3880_cov_2.158431"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..2246
                     /locus_tag="PK198_23480"
     CDS             342..2246
                     /locus_tag="PK198_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRF41784.1"
                     /translation="MATDPKVVLQQYWGYPAFRSMQEDIILSVLNGKDTLALLPTGGG
                     KSICFQVPALCREGICIVVSPLIALMKDQVFNLRNRGIAAEAIYSGMRAKDIDRILDN
                     CVYGNTKLLYLSPERLTTDIARERISRMKVNLLAVDEAHCVSQWGYDFRPPYLLIPQL
                     RELIPDVPVLALTATATPEVVTDIQEKLAFRKPHVFQKSFARSNLAYVVRYTEGKEEQ
                     LAEILQKVPGCGVVYARNRRKTKDLAVMLQRKGISADYYHAGLDGETRSQKQDAWINN
                     QTRIMVSTNAFGMGIDKPDVRVVVHTDLPDSLEAYFQEAGRAGRDEKKSYAVLLYNDS
                     DRRELERNFEQSYPTIEYIRQVYRALGSYYQMALGSGKGESYDFDIGRFCEIFKLEPA
                     RTYSCLKILEQAAWLTLTEAVWTPSTLQIHADKETIYDYMLRNPKSDLVLKSILRTYQ
                     GAFQHPVNIKEEQLARFLQMPTNELVQALELMSKEGMLEYQPRKDKPQIVFLEERVDP
                     ANLTLDMAMYAFRKQRQEKRIRKSVEYATRNECRSRMLLAYFGEEESKTCGVCDVCLE
                     QKKGALHEQRFEQIQNSIKDKLAAGPQNIHQIVALFPASQKEDVLSVIEYLLDEQFMT
                     DSEGVLHWNA"
     gene            2243..3379
                     /locus_tag="PK198_23485"
     CDS             2243..3379
                     /locus_tag="PK198_23485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF41785.1"
                     /translation="MNREHSYIIAAVTNDLHQDQRMQRICTTLQDAGYKVKLTGRRRH
                     GSRALPPQAYEQTRLRCWFERGKLFYLEYNLRLLLYLLGQSFDAVCAVDSDTLIACFV
                     AARIKGKPCVLDAHEYFSETPEVAQRPVVKTIWEAVARWIIPRLRHCYTVGEALAEVL
                     SKRYGVDFETIRNAPLKQVAPAPSSVVHSDRPFIVLYQGMLNAGRGLEAMLEAMQMLE
                     NVQLWLAGEGDLSEELRRQAQTLIGQDKVRFLGFVPPAELPQLTSQAHIGINLLEAGS
                     LSYWYSLANKALDYIHAGVPSIQMDFPEYGKINEKWEVFTCIPDLRPGTIAAAVRALQ
                     QDRDKYEWMQEQCAIAANELCWQNEAPKLLNLYERVFRTHADRR"
     gene            complement(3354..>3564)
                     /locus_tag="PK198_23490"
     CDS             complement(3354..>3564)
                     /locus_tag="PK198_23490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF41786.1"
                     /translation="VNTISPGYIGTDMVMAVAQEYRDKIVQTIPVGRLGGTWEVAHLV
                     SFLASKEASFITGANVAINGGQHVF"
     assembly_gap    3565..3670
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3671..>3880)
                     /locus_tag="PK198_23495"
     CDS             complement(<3671..>3880)
                     /locus_tag="PK198_23495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF41787.1"
                     /translation="QIEKEHGTVDVLVNNAGITRDGAFRKMSYENWDAVLKSNLYSVF
                     NCCHHVVSHMLQQNYGRIINISSVN"
BASE COUNT          994 a          932 c          976 g          872 t
ORIGIN      
        1 cgtattattt cagtagaaca agaaaacaga ctttcaaaaa aaatgaacct gcaacttttt
       61 gcagaccgct gaatttttat aaaaaactgg taatcaatat tttgaaacct ttttttataa
      121 aaaaatgtaa taacaagaca ttacaataaa aaaacttggc ccaaaggtag tatctccggt
      181 tcaagatttc gcaaaaagca tgacatatta tggaaaaaag tgaaagacgg tgcaaaaatg
      241 agccatttct gcaagcgagt caagtgtttc taatgttttt tttaccttgc atcaactttt
      301 tctatgacaa atatttgtaa acaattcaaa ttcaacgccc tgtggccact gatccaaaag
      361 tcgtacttca gcaatattgg ggctaccctg cgtttcgatc catgcaggaa gacatcatcc
      421 tgtcggtact gaacggaaag gacactttgg ccctgttgcc taccggagga ggtaaatcca
      481 tttgttttca ggtgccggcc ttatgcagag agggcatttg tatcgtcgtt tcgccattga
      541 ttgccttaat gaaagatcag gtgttcaatc tgagaaacag aggtatagcc gccgaggcca
      601 tctattccgg catgcgcgcc aaagatattg atcgcatttt agacaattgt gtttacggca
      661 ataccaagct gctctactta tctccggagc gattgacaac cgacatagcc cgcgaacgca
      721 tcagccggat gaaggtcaat ctgctggcgg tggacgaagc gcactgcgtc tctcagtggg
      781 gttatgattt caggccgccc tacctgctca ttccgcaatt gagggaactg attccggatg
      841 tgcctgttct ggccctgacg gctacggcta cccctgaagt ggtgaccgac attcaggaaa
      901 agctcgcgtt caggaagccc catgttttcc aaaagagctt tgcccgcagc aatctcgctt
      961 atgtggtacg ctacacagag ggcaaggagg aacaattggc cgaaatattg caaaaggtgc
     1021 ccggctgcgg cgtggtgtat gcccgcaatc gccggaaaac gaaggacttg gccgtgatgc
     1081 tgcaacggaa gggcatttca gcagattact accatgccgg cttggatgga gaaacccgtt
     1141 ctcaaaaaca agacgcctgg atcaacaacc aaacccgcat catggtgagt accaacgctt
     1201 tcggaatggg cattgacaaa cccgatgtgc gcgtagtggt acataccgac ctgcccgaca
     1261 gcttagaggc ttattttcag gaggcaggcc gggctgggcg cgacgagaaa aaatcgtacg
     1321 ccgtattatt atataatgac tccgaccgga gagaactgga gcgcaacttc gagcaatcgt
     1381 atcccaccat agaatatata aggcaggtgt atcgggcgct gggcagttat taccagatgg
     1441 cgttaggcag cggcaaaggc gagagttacg atttcgatat cggccgattt tgcgagattt
     1501 tcaaattgga acccgcccga acgtacagtt gcctgaaaat tttggagcag gccgcctggc
     1561 tcaccctgac cgaagccgtc tggacaccct ctactttgca aattcacgcc gacaaggaaa
     1621 cgatttatga ctatatgttg cgcaatccca agtcggacct cgttctgaaa agcatcctcc
     1681 gcacctacca gggcgctttt cagcatccgg taaacatcaa agaggagcaa ctggcccgtt
     1741 ttcttcagat gccgaccaat gagttggttc aggcactcga actcatgtcg aaagaaggta
     1801 tgctggaata ccagccccga aaagacaagc cgcagatcgt ttttttggaa gagcgcgtgg
     1861 atccggccaa cctcacctta gacatggcga tgtacgcgtt cagaaaacaa aggcaggaaa
     1921 agcgcatccg caaatcggtg gagtatgcta cacgtaacga atgtcgcagc cgcatgttgc
     1981 tggcttattt cggcgaggag gaaagcaaaa cgtgcggcgt gtgcgatgtg tgtctggagc
     2041 aaaagaaggg cgccctgcac gaacagcggt ttgagcaaat ccaaaactcg ataaaggaca
     2101 agttggccgc cgggccgcaa aacattcacc aaatagtggc cctgtttcca gcatcgcaaa
     2161 aagaggatgt gctcagcgta atcgaatacc ttctggatga gcagtttatg acagattcag
     2221 agggtgtttt acattggaac gcatgaaccg ggaacactca tatatcatag ctgccgtcac
     2281 caacgacctg catcaggacc aacggatgca acggatttgc actacactgc aagacgccgg
     2341 ttacaaggtg aaactgaccg gcagacggag gcacggcagc agggctttgc ccccgcaagc
     2401 ctacgagcaa acgcgattga gatgctggtt tgagcgaggc aagttgtttt atctggaata
     2461 caatctccgc ttgttgttgt acctgttggg acagtctttt gatgccgttt gcgccgtaga
     2521 ttccgacacg ctgattgcct gctttgtggc tgctcgcatc aaagggaagc cctgtgtgtt
     2581 ggatgcgcac gagtattttt cagaaacgcc ggaagtggcg cagcgtccgg ttgtaaaaac
     2641 gatctgggag gctgttgcga gatggattat acctcggctt cgccattgct acacggttgg
     2701 agaggcattg gcagaagtac tttctaaaag atacggcgtt gattttgaaa ccataagaaa
     2761 cgcgcctttg aaacaagtcg cccctgcgcc aagctccgtc gttcactccg acaggccgtt
     2821 catcgttttg tatcagggca tgttgaatgc gggcagagga ttggaggcca tgctggaagc
     2881 catgcaaatg ttggaaaatg tgcaactctg gcttgccggc gagggagatt tgtcggaaga
     2941 gcttcgccga caggcgcaaa ccctcatcgg gcaggataag gtcaggttcc tcggttttgt
     3001 gccgcccgcc gaactcccac aattgacctc gcaggcgcac atcggcatca acctgctgga
     3061 agccggcagc ctgagctatt ggtactcttt ggccaacaag gcgttggatt acatacacgc
     3121 cggcgtgccg tccatacaaa tggattttcc cgagtacgga aagatcaacg aaaaatggga
     3181 ggttttcacc tgcatacccg atctgcggcc cggcaccatt gccgccgccg tgcgcgcact
     3241 tcagcaggat cgcgataaat acgaatggat gcaagagcag tgcgccattg cagcaaatga
     3301 attgtgttgg caaaacgaag cccccaaact actgaacctg tacgaacgcg ttttcagaac
     3361 acatgctgac cgccgttgat cgctacgttg gcgccggtaa taaaggatgc ctctttggat
     3421 gccaaaaacg acaccaaatg cgccacttcc caagtgccgc ccaagcgccc taccggaatg
     3481 gtctgtacaa ttttgtcccg gtactcctgc gcaacggcca ttaccatgtc ggtgccgatg
     3541 tagcccggcg agatggtgtt gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn cattcaccga tgagatgttg atgatgcgcc catagttttg ttgcagcatg
     3721 tgactgacaa cgtgatggca acagttgaac acgctgtaca gattgctttt cagaacagca
     3781 tcccagtttt cgtagctcat tttgcgaaaa gcgccgtcgc gggtaatgcc ggcgttgttg
     3841 acgagcacgt caaccgtacc gtgctctttt tcaatctgtt
//
LOCUS       DAORLF010000442         3876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12802_length_3876_cov_1.979848, whole genome shotgun sequence.
ACCESSION   DAORLF010000442 DAORLF010000000
VERSION     DAORLF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_3876_cov_1.979848"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>246)
                     /locus_tag="PK198_23500"
     CDS             complement(<1..>246)
                     /locus_tag="PK198_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRF41788.1"
                     /translation="ILTIIMLWFIEKTWRLAETAALRIGLGLLLAALLAQVLLGILTV
                     INCVGSIPVALGVLHQAGGVLLLTAVLYVYFQVRQVKN"
     assembly_gap    247..382
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..>738)
                     /locus_tag="PK198_23505"
     CDS             complement(<383..>738)
                     /locus_tag="PK198_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRF41789.1"
                     /translation="FWRKGAISPLLMRRLGITVLLAAVVAAFGWIMVASGLVNRPWVN
                     AYKLTLHLSLALLVYCYLWWTILLVSPPKVQVFHTPLLKMWGKRLIALTAIQIALGGM
                     MSGMKAGLFYPTWPDM"
     assembly_gap    739..792
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<793..1094)
                     /locus_tag="PK198_23510"
     CDS             complement(<793..1094)
                     /locus_tag="PK198_23510"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRF41790.1"
                     /translation="MSNNHSIFTSPQAKFQSALKLWLLIGMVMIFVQIVVGGITRLTG
                     SGLSITKWEIVLGALPPINAEQWETAFDLYKATPQYQKINQGMSMGEFKFIYFWEY"
     gene            complement(1800..3050)
                     /locus_tag="PK198_23515"
     CDS             complement(1800..3050)
                     /locus_tag="PK198_23515"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRF41791.1"
                     /translation="MLRISTLLLAILPLAGLLAQNADQDAKLRRLADRLAHRYIITDG
                     HVDLPYRLKVQNFRLTKEFIGIPIETTDGDFDYVRAKKGGLDAPFMSIYIPSSYQQSG
                     GAVQLADSLIDMVRSIAQAHPDKFAVAVSPKDVERNFRKGLISLPLGMENGAPIEDKL
                     SNVAYFHRRGIRYITLTHATDNLICDSSYDTTRTWNGLSPFGKEVVREMNRVGIMVDI
                     SHVSDSAFYQTLQVSSVPVIASHSSVRDYTPGFERNMNDDMLRALAKNDGVIMINFGS
                     TFLDSKIQEAENELRAKLSELLQEKGLNWRDEAAKPIIEAFQKEHPKLYSDVEMVATH
                     IDHVRRVAGIRHIGLGSDYDGVGDSLPTGLKDVSQYPNLIYVLLKRGYSKQDIARICY
                     KNLWRVWNAAEKAGRSSSPQPALK"
     gene            3254..3802
                     /gene="hpt"
                     /locus_tag="PK198_23520"
     CDS             3254..3802
                     /gene="hpt"
                     /locus_tag="PK198_23520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRF41792.1"
                     /translation="MKTVQAHDLHFSLFLTEEDIQRRVQELGARLRAQYGDKRPVFLG
                     VLNGSFMFAADLVRAFGADCELSFIKLSSYRGLHSTGDVSTLIGLETDLQDRHVIIVE
                     DIVDSGKTLYSLLPDLQRLQPASVAIAALLQKPDMLEFPLEVQYVGFSIPPHFVIGYG
                     LDYDGLGRQLPAIYQLIKNNED"
BASE COUNT          914 a          890 c          986 g          896 t
ORIGIN      
        1 atttttaacc tgacggactt gaaaatagac gtacaaaacg gccgtcagca ggagtacgcc
       61 gccggcctga tgcaaaacgc ccaatgcaac gggaatgctt cctacacagt tgattacggt
      121 cagaattccc aacaaaacct gcgccagcaa agccgcgagc aacagcccga gtccgatacg
      181 cagagccgcc gtttcagcca gccgccacgt tttttcaata aaccataaca taatgattgt
      241 caatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nncatatccg gccaggtggg gtaaaacaaa cccgctttca
      421 tgcccgacat catgccgccc aacgctatct gtatggctgt gagggcaata agtcgttttc
      481 cccacatttt caacaaaggt gtgtggaaaa cctgcacttt gggtggagaa accaacaata
      541 tcgtccacca gaggtagcaa tacaccaaca aggccaaaga aaggtgcagg gtgagtttgt
      601 aggcgttcac ccaggggcga ttcaccaaac cgctggccac catgatccat ccaaacgcag
      661 ccaccactgc cgccagcaat accgtgatgc cgagccgtcg catcaatagc ggactgatgg
      721 cgccttttcg ccaaaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnatgtattc ccaaaaataa atgaacttga actcgcccat gctcattccc
      841 tggttgatct tttggtactg cggcgtggct ttgtacagat cgaaagcggt ttcccattgt
      901 tcggcgttaa tcggcgggag ggcgcccagc acgatttccc atttggtgat ggacaggccg
      961 gaaccagtga gccgggtgat gccgcctacg acaatttgta caaaaatcat gaccatgccg
     1021 atcagcagcc agagtttgag ggctgattga aacttggctt ggggagatgt gaaaatggaa
     1081 tgattgttgc tcataaaagt aaaggtaaaa atttcatcgc cgggccagat accaagcgac
     1141 tattttctga tcgctacgct aagtaagctc ctgtttatct gttttttaga cgctggaaga
     1201 cgcagatttg tatgatcgtc atgccctcta tgagcagcga aaatcaaaag aatcataaca
     1261 aggtcagcgt caaaaaatat gagcgccgga agccggctgt ttccaatcag aactgccttc
     1321 cttgagttgc ttacatttaa acaattcaga gagtcccgtc gaaacacgct tcccgcgaca
     1381 aagcgaaaac aaaagtacgc ccaaatgttt gcgcaacagc gcccgtttcc acattcctca
     1441 ctgttgttaa taatcttttt taaataaatt tttaaatccc ctgtcatcat caggtccgtg
     1501 gaaatgtaga gaacttttta ccccaaaatt tggcggtcgg gttatcccca gtcatgccca
     1561 tgttatccac aggttaattc agtattaact catccaatgt caggccattg ccgatttatc
     1621 cacaatgagt ggatagggat gagtatggaa aaaaagtgat ttcccaccgg ccgaattttt
     1681 gtggaaagta tgtcgaaaaa cagtgcggta ttccacgcta tatcgggaat gccttgttgt
     1741 gaaagggcgt ttttcacggc aatcttggta agtgtggaaa agatggcagt tatctacact
     1801 tatttcaacg ccggttgtgg agaagaggag cggcccgctt tttcggcggc attccacacc
     1861 cgccagaggt ttttgtagca aatacgggca atgtcctgct tgctgtagcc gcgcttgagc
     1921 aatacataaa taaggttggg atattgcgaa acgtctttga ggccggtggg cagcgaatcg
     1981 ccgacgccgt cgtaatcgga gccgagaccg atgtgccgga tgccggccac gcggcgaacg
     2041 tggtctatgt gagtggccac catttccaca tcggagtaga gtttggggtg ttctttctgg
     2101 aatgcttcga tgatgggttt ggctgcctcg tctcgccagt tgagaccctt ttcctgcaac
     2161 agttcggaga gtttggcgcg cagttcattt tcagcttcct gtattttgga atccagaaaa
     2221 gtagagccga aattgatcat aatcacgccg tcgtttttgg ccaatgcgcg cagcatgtcg
     2281 tcgttcatat tgcgttcgaa gccaggcgta taatcgcgca cggaggagtg cgaggcgatg
     2341 accggcacgg aactgacttg gagggtttgg tagaaagcgc tgtcggaaac atgcgaaata
     2401 tccaccatga tgcccacgcg gttcatctcg cgcaccactt ctttgccgaa aggactgagg
     2461 ccgttccagg tgcgggtggt gtcgtaggag gagtcgcaga tgaggttgtc ggtagcatgt
     2521 gtgagggtga tgtagcggat gccgcgccgg tgaaagtagg ccacattgga gagcttgtct
     2581 tcgatgggcg cgccgttttc cattcccaaa ggcaaggaaa tgaggccctt gcggaaattg
     2641 cgttctacat ctttgggtga tacggcaacg gcaaatttat cggggtgtgc ctgcgcgatg
     2701 ctgcgcacca tatcaatgag cgagtcggcc aattgtacag ccccgccact ttgctggtaa
     2761 gaggaaggaa tgtaaatgga catgaagggc gcatccaagc cgcctttttt ggcgcgcaca
     2821 tagtcaaaat cgccatctgt ggtttcaatg gggatgccga tgaattcttt ggtgagccgg
     2881 aagttttgca ctttgaggcg gtagggcagg tccacatggc cgtcggtgat gatgtagcgg
     2941 tgggcgaggc ggtcggccaa gcggcggagt tttgcgtcct gatcggcatt ttgagccagg
     3001 aggccggcca aaggcaatat ggccagcagg agagtgctta ttctgagcat gatgtatgaa
     3061 attggtggaa atataggcaa aggtacgaaa tagcaagaac ccaaatggaa caagagcgcc
     3121 acctgcgaag atcaataaat ctgcctgttt gttgtcttgg tagtggggca aaaatgacca
     3181 ctttattttt ttatcgcccc ttatatccaa aaaagccgcc aaatttgcat tttctaaaat
     3241 caacatctcc cgcatgaaaa cagtacaggc gcacgatctc catttcagtt tgttcctcac
     3301 cgaagaggat attcagcggc gcgtacagga gttgggcgcc cggctgcggg cacaatatgg
     3361 cgacaaacgc cctgtgtttc tgggggttct gaacggctcc ttcatgtttg cggccgattt
     3421 ggtgcgcgct ttcggggccg attgcgaact gtcgttcatc aaactttcat cctaccgggg
     3481 cctgcactca acgggcgatg tttctacgct tatcgggctg gaaaccgacc tgcaagaccg
     3541 ccacgtcatc attgtggaag acatcgtgga ttcgggcaag acgctgtatt cgctgctccc
     3601 cgacttgcaa agactgcagc cggcctcggt ggccattgcc gccctgctgc aaaaaccgga
     3661 tatgctggag tttcctctgg aagtgcagta tgtgggcttt tccataccgc cgcatttcgt
     3721 catcggctac gggttggatt atgacgggct ggggcggcag ttgccggcca tctatcagtt
     3781 gataaaaaac aacgaggatt gatctctgac tttttgtcgg gaaagcgggt gaagttcgta
     3841 gcatacttcc tccaaaccat agccttgggt ctggtc
//
LOCUS       DAORLF010000443         3866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12845_length_3866_cov_2.282603, whole genome shotgun sequence.
ACCESSION   DAORLF010000443 DAORLF010000000
VERSION     DAORLF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12845_length_3866_cov_2.282603"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="PK198_23525"
     CDS             complement(<1..1494)
                     /locus_tag="PK198_23525"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HRF41793.1"
                     /translation="MRFSHFAWLLLALALWGCKKETATVERPHDPWVFRSVLDSLPRM
                     LTVALHDDVWAAYSAQTGALYKVWKGGVNFDGAVYTTAHGPQPSTLGDAYFHNDYPEP
                     WTVALNGTDETPRIQYRGHSFDKKGKVSINTELILSNGAVVKVHERPEYITNTTGQPG
                     MERVFETEGLPSGAQLKFMFNLSSIAHSTNIETDGTLTLGETTTRQVDDREVMDLNAV
                     LTLQPKGKTRLAAYFTKPSVPNPNKIVGAEEEEEVPLGFRLIARSDCKTCHNTYVKTI
                     GPSYVDIAKKYMNTPENTAMLVQKVKLGGAGVWGEAAMSAHPDASDADLTTMVSYIMA
                     LDSVEEAAFVQQHRNSGPVTNARAADKTIGEDNLLPGALTTVWTSKKTLTKLSDVDRG
                     AEPVFVGIMPEVKMGSGDFGPLTDNFAVRVEGYLRIPKTSNYLFRLKSDDGSQLFIGN
                     KLIIDHDGLHGDAPKDGEVALEEGFHPFRIDFFQGGGGKALFFEWSSF"
     gene            complement(1547..>1919)
                     /locus_tag="PK198_23530"
     CDS             complement(1547..>1919)
                     /locus_tag="PK198_23530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41794.1"
                     /translation="NTAKEPIAIVTADHITGEVLRTEYKFNGIISSANDLLEANKSVM
                     AYPNPTGDRLFIELQNLSWGEYEFSMFDAAGRSVMRRQVQFQVGAPAEIDVQSIRSGA
                     YIYTLTDKGGQIRYAGRLVKQ"
     assembly_gap    1920..1946
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1947..2678)
                     /locus_tag="PK198_23535"
     CDS             complement(<1947..2678)
                     /locus_tag="PK198_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41795.1"
                     /translation="MNRIISFFLFFALAGSLSAQITVTNASFPAAGDTLRVAFDTTSA
                     GIVITPPGGNQTWNFSALTAAFTRTSVVKPASEGESAANFPAATLVLEGQNGDSYFRV
                     TPNVYELIGFDGNDPAGLGVPFVTAFNPAIQERRAPLNFFEINTTNGALLVPFAANQL
                     PQAILNLLPIVPDSIRIRLASNRTDLVDGWGTLTIPGGTYEVLRERRTQYSDTRVEVK
                     IGVFPWTDVTALIPLPDVGLGRDTTL"
     gene            2967..>3866
                     /gene="crtI"
                     /locus_tag="PK198_23540"
     CDS             2967..>3866
                     /gene="crtI"
                     /locus_tag="PK198_23540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRF41796.1"
                     /translation="MATKKAIVIGSGFAGLSAATSLAAEGFDVTILEKNSVAGGRARK
                     YEADGFMFDMGPSWYWMPDVFEQYFARFGKKPSDYYDLVRLDPSYSVIFGQDQRMDLP
                     ASMEGLYALFEQHEPGSSAQLRRFLAEAKYKYEVGMADFVHRPSHSILEFADIRILLS
                     LFRLQMFQSISSHIRKLFKNEQLIQLLEFPVLFLGATPEKTPALYSLMNYADMALGTW
                     YPMGGMHKIVEGMVSLAEEKGVKIRLGEEVKNIFVPNGHATKVITQNAEYEADVVISG
                     ADYHHTEQQLLQAPQRNYPQEYWN"
BASE COUNT          880 a          958 c         1065 g          936 t
ORIGIN      
        1 gaaggaactc cactcgaaga aaagcgcctt gcccccgccg ccctgaaaga aatcaattcg
       61 gaaagggtgg aaaccctctt cgagggccac ttcgccgtct ttgggcgcgt cgccatgcaa
      121 gccgtcgtgg tcaatgatga gtttgttgcc gatgaagagt tgcgagccgt cgtcgctttt
      181 gaggcggaag aggtagttgc tggttttggg aatgcgcagg tagccctcta cgcgcacggc
      241 aaagttgtcg gtaagcggcc cgaaatctcc actgcccatt ttcacttccg gcatgatgcc
      301 cacaaagacc ggctcggcgc cgcgatccac gtcggagagc ttcgtcagtg ttttcttgga
      361 ggtccaaacc gtagtcagag cgccgggaag caagttgtct tcgccgatgg ttttgtcggc
      421 agcgcgggca ttggttacgg ggccgctgtt gcggtgttgt tgcacaaagg ctgcttcttc
      481 caccgagtcc aaagccatga tgtaactgac cattgtagtg aggtctgcat ccgaggcatc
      541 ggggtgggcg ctcatggccg cctcgcccca aactcctgcg ccgccgagct tcactttttg
      601 caccagcatg gccgtatttt cgggcgtgtt catgtatttt ttggcaatgt ccacataaga
      661 tggcccaatg gttttgacgt aggtattgtg acaggttttg caatcgctgc gggcgatgag
      721 gcgaaaacca agcggcactt cctcttcctc ttcggcgccc actattttgt tgggattggg
      781 cacggaaggt ttggtgaaat aggctgccag ccgggttttg cccttgggtt ggagggtgag
      841 caccgcgttg aggtccatca cttcccgatc atccacctgc cgggtagtcg tctcgccaag
      901 tgtcagcgtc ccatcggttt cgatgttcgt ggaatgcgca atggaactga ggttgaacat
      961 aaatttcaac tgcgcgccgg aaggcaaacc ctccgtttca aaaacgcgct ccatgccggg
     1021 ttggccggtg gtgttggtga tgtattccgg gcgttcatgt acttttacca cggcgccgtt
     1081 gctcaaaatg agttcagtat tgatgctcac tttgcctttt ttgtcaaaac tatgcccccg
     1141 gtactgaatg cgcggcgttt cgtccgttcc gttcagcgct acggtccagg gttccgggta
     1201 gtcgttgtgg aaatacgcat ctccgagggt gctgggctgc gggccgtgcg cggtggtata
     1261 aacagccccg tcgaaattga cgccgccttt ccagacctta tacaaagcgc cggtttgagc
     1321 gctgtaggcg gcccacacgt cgtcgtgcag agccacggtg agcatgcgtg gaagcgaatc
     1381 caacacggag cgaaagaccc agggatcgtg cgggcgttcg acggtagcgg tttctttttt
     1441 acaaccccag agggccaggg ccaagagtag ccaggcaaaa tgtgaaaatc tcatgagcga
     1501 aacaggttat tgtgcgatgt ttgaaaatac gagtagctgt attttatcat tgcttcacca
     1561 gacgaccggc gtagcgaatc tggccgcctt tgtcggtgag cgtataaata taggcgcctg
     1621 aacggatgga ttgtacatca atttctgccg gcgcacccac ctggaattgc acttgccggc
     1681 gcattacaga ccggcctgcg gcatcgaaca tgctgaattc atactcgccc caagacaggt
     1741 tttgcaattc tatgaacaga cggtcgccgg tcggattggg ataggccatg acagacttgt
     1801 tggcttccag aaggtcgttg gcagaagaaa taatgccgtt gaatttgtac tcagtccgca
     1861 atacctctcc cgtgatatga tcggcagtga cgatggcaat gggttctttg gcagtattgn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnncagc gtagtgtccc gacccaaacc cacatcggga
     1981 agtggaatga gcgcggtaac gtccgtccag gggaaaacac cgattttcac ttctacccgc
     2041 gtgtcggaat actgcgttct gcgttcgcgg agtacttcat aagtaccgcc gggaatggta
     2101 agcgtacccc agccatccac cagatccgtt ctattggacg ccagtcgaat gcgtatcgaa
     2161 tccggtacaa tgggcaacag attaaggatg gcttgcggca actgattggc agcaaatgga
     2221 accaacagcg cgccgttggt cgtgttgatc tcaaaaaaat tcaatggcgc tctgcgttcc
     2281 tgaatggcag ggttgaatgc agtgacaaaa ggcacgccaa gtccggcagg gtcattgccg
     2341 tcgaagccga taagttcata aacattaggc gtgacgcgaa aataactatc tccgttttgc
     2401 ccttcaagta ccagcgtggc ggcaggaaaa ttggctgcgc tttcgccttc cgaggccggt
     2461 ttgaccaccg aagtgcgggt gaaagcggcc gtcagtgcgc tgaaattcca ggtttgattg
     2521 ccgcccggag gggtaatgac aatacctgcc gaagtggtat cgaaggcaac gcgcagggta
     2581 tctcccgcag ccgggaagga tgcgttggtc accgtgattt gggcgctgag gctgccggcc
     2641 agagcgaaaa acaaaaagaa gctgatgatg cgattcatcg tttttgggta attttttgat
     2701 ggaagaaacg acgtcggctt ccggaaatgg aagctttcga ctttgtaatg gggcaaagct
     2761 actgtttttg aagttttttc cgattcaaaa tttttatgct catcagggca tccaaacctg
     2821 ttgggatgga gtgccggtat atgtacaggg cgccgacaaa gcacctcagc agcacctcta
     2881 agttattttt gtttaaatat ttttttaaaa ttcttaaaca aaacacacct ccccctgttt
     2941 cgctcatcaa caaaaaacat acagtcttgg ctaccaaaaa agcaattgtc atcggttcgg
     3001 gtttcgcggg gctttcagca gctacatcct tggcggcaga aggttttgac gttactattc
     3061 tggaaaaaaa cagtgtggca ggcggccgtg cgcgcaaata cgaggccgac ggtttcatgt
     3121 tcgacatggg gccgtcctgg tattggatgc cggatgtgtt tgagcagtac tttgcacgct
     3181 tcggaaaaaa gccctccgat tattacgatc tcgttcgcct cgatccttct tactctgtca
     3241 ttttcgggca ggatcagcga atggatttgc cggcttcgat ggaaggtctg tatgccttgt
     3301 ttgaacaaca cgaaccgggc agcagtgcgc aattgcgtcg ttttctggcg gaagccaaat
     3361 ataaatacga ggtgggcatg gccgactttg tacaccgccc cagccactcg attctggaat
     3421 tcgccgatat ccgcatcttg ttgagtttgt tccggttgca gatgtttcaa tccatatcgt
     3481 cgcacatcag aaaattgttt aaaaacgaac aactcattca gttgctggag tttccggtgc
     3541 tgtttctggg cgctacccct gaaaaaacgc cggccttgta cagcctgatg aactatgccg
     3601 acatggcttt gggtacctgg tatccgatgg gcggtatgca caaaatcgtg gagggcatgg
     3661 tgagccttgc ggaagaaaag ggcgtaaaaa tcagactggg cgaagaggtc aaaaacatct
     3721 ttgtccccaa cggccatgca accaaagtta ttacccaaaa tgctgaatac gaagcagatg
     3781 tagtcatttc cggcgccgat tatcaccata cagagcaaca actgctgcaa gctcctcaac
     3841 gcaattaccc tcaggaatac tggaac
//
LOCUS       DAORLF010000444         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_12945_length_3845_cov_2.112137, whole genome shotgun sequence.
ACCESSION   DAORLF010000444 DAORLF010000000
VERSION     DAORLF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12945_length_3845_cov_2.112137"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK198_23545"
     CDS             <1..350
                     /locus_tag="PK198_23545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF41797.1"
                     /translation="VRYYFGAADLVVQPYRTATQSGISQLAYHFEKPMVVTHVGGLPE
                     IVEHGVSGYVVPVNENAIAEAIADYFNHHRAATMLQAVREAKKRFSWSAMSAALLHLY
                     EQLKQHGKKNNQQ"
     assembly_gap    382..416
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <417..851
                     /locus_tag="PK198_23550"
     CDS             <417..851
                     /locus_tag="PK198_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41798.1"
                     /translation="GIFLALLLSAALWGWPHIFGRETSFSRSVLAAIELFLIWLVVTS
                     TVRAIHRIRPKIELWRLYLAGIVTSFTGIILKEIVWSGKRYAQEGGVAFSFKKLLFYL
                     AVAFIASSIALIRLRVRDRKTGQVLEIAFIGVVMFLFFHFVK"
     gene            940..1515
                     /locus_tag="PK198_23555"
     CDS             940..1515
                     /locus_tag="PK198_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRF41799.1"
                     /translation="MPSTINSIIQNSIAAKQAMLADGLLLKTLESAVQACLATYRRDG
                     KILLCGNGGSAADAQHIAAELSGRFYKDRPPLYAEALHVNSSYLTAVGNDYGYDEVYA
                     RMVRAAGRPGDVLVAISTSGNSPNVVRAAQAAREIGMYVIGMTGAGGGQLAALSHHLL
                     AAPSKDTPRIQECHILMGHILCELIERELFQ"
     assembly_gap    1550..1580
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1799..1969
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2090..2638
                     /locus_tag="PK198_23560"
     CDS             2090..2638
                     /locus_tag="PK198_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF41800.1"
                     /translation="MFRWLCSFVFYKLMGWRMEGAFPFHLPKFVIAVVPHTSNWDFPI
                     GIMVRSIFRHSDLNFVGKDALFRPPHGWFFRWLGGYPVDRSKSNNFVDSVVDIFNREK
                     EFRLTLAPEGTRKKVDKLKTGFYYIAVGAKVPIVLVKFDYGNKCVAIAEPFYPTGDAE
                     ADMEFISNHFRGVRGYHPELSF"
     gene            complement(2640..3023)
                     /locus_tag="PK198_23565"
     CDS             complement(2640..3023)
                     /locus_tag="PK198_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRF41801.1"
                     /translation="MEKVRIDKWLWSVRIFKSRTIATDACKEGKVKLNGISAKSSQLI
                     QVGDQVEVKKEGFNMQYKVLNLIEKRVGAPIAQACYEDLTPAEELNKYKDWFVGKAGA
                     EYREKGAGRPTKKERRVIDDFKDHR"
     gene            complement(3027..>3525)
                     /locus_tag="PK198_23570"
     CDS             complement(3027..>3525)
                     /locus_tag="PK198_23570"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF41802.1"
                     /translation="LLWEGYEGLSLGQSAHNGSLRIASGVVNAQGNLPVAGKFVIDMT
                     SLKVEDMPAGDMGNAKLTRHLLNADFFDAAKYPQAVFEITQSRAITADSINITGNLTL
                     RGTTKSVSFPAKVSAANGMFSAVTPKFYINRKDWGMFYRSEDSLGDDLIRPEMGITLA
                     IVAKK"
     assembly_gap    3526..3553
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          770 a         1011 c          957 g          842 t
ORIGIN      
        1 acgtacgcta ctatttcggc gccgccgacc tcgtggtgca gccctatcgc acggccaccc
       61 aaagcggcat ttcgcaattg gcctaccact ttgaaaaacc gatggtcgtc acccatgtag
      121 gcggcctccc cgaaatcgta gagcatggcg tttcgggcta tgtggtgccg gtgaatgaaa
      181 acgccatcgc ggaagccatc gccgattatt tcaaccacca tcgggctgca acgatgctgc
      241 aggcagtgcg ggaagcaaaa aaaagattct cctggtcggc catgagcgcc gccctgctcc
      301 acctgtacga acaactcaaa caacatggca aaaagaacaa ccaacaatag agaaagcgcc
      361 gttgagcaac agtatctcaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca
      421 tatttctggc gctgctgctc tcggcggcac tttggggctg gccgcatatt ttcggccggg
      481 aaacttcctt ttcgcggtca gttctggcag ccatagaact gtttctcatc tggttggtcg
      541 tcaccagcac cgtgcgcgcc atccaccgca tccgccccaa aatcgaactc tggcggctct
      601 atctggcggg catcgtcacc tcgtttaccg gcatcatcct caaagaaatc gtgtggagcg
      661 gcaagcggta cgcacaggaa ggcggcgtag cgttcagttt caaaaagctg cttttttatc
      721 tcgccgtggc attcatcgct tcctccatcg cgctcattcg cctccgcgtg cgcgaccgaa
      781 aaaccggcca ggtgttggaa atcgctttca tcggcgtggt catgtttttg ttttttcatt
      841 ttgtgaagta acggcagcta aagtacggcg actaaagtcg ccggtaccgc gtgccgatat
      901 gctaaaaaat aatacagcga ctaaagtcgc cgctaccgta tgccatccac catcaactcc
      961 atcatacaaa acagcatcgc agccaaacag gccatgcttg ccgacgggct gctgctcaaa
     1021 acgctcgaat cggccgtaca ggcttgcctc gccacctatc gccgcgacgg caaaatcctc
     1081 ctctgcggca acggaggcag tgccgccgat gcccagcaca tcgccgccga gctgtcgggc
     1141 cgattttaca aagaccgccc gcccctctac gcagaagcgc tgcacgtcaa cagttcttac
     1201 ctcaccgctg tgggcaacga ctacggctac gacgaggtgt acgcccgcat ggtgcgcgct
     1261 gccggccgcc ccggcgatgt gttggtggct atttctacct ccggcaattc gcccaacgta
     1321 gtgcgcgccg cccaagccgc ccgcgaaatc ggcatgtacg tcatcggtat gaccggcgca
     1381 ggcggaggtc aactcgccgc gctgtcgcat cacctgctgg ccgcgccctc caaggacacg
     1441 ccgcgcattc aggaatgcca tatcctcatg gggcatattt tgtgcgagtt gattgaaagg
     1501 gagttgttcc agtaggcgag ggttcggtag gcggtcggcg gtgtgcagtn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn cgggggttcg gtaggcggta tgcggcgggt tagtggtgag
     1621 tgcgtgaggc gtgagcaaca cggaacgctg aaccttaaac cctaaacctt gaacccggaa
     1681 ccggtaggcg gtactcgata ggcagtgcta agccggggga acctcgaacc tcgaaacgcg
     1741 aatttttttt gacgctgatc tttatgattt ttatgattgg cgctgctcgg agaggggcnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gccttcagtg
     1981 ggtgaggggt gagtgcgtga ggggtgggca acccgaaacc cgaaacccag aactttgacc
     2041 ccggaatccg aaatccgaag tatcttcgcc cttctaaaaa acacgacgta tgttcagatg
     2101 gctttgctct tttgtgttct ataaactcat gggttggcgc atggaaggcg cgttcccctt
     2161 ccacctgccc aaattcgtca ttgccgtagt gccgcatacc tccaactggg atttccccat
     2221 cggtatcatg gtgcgctcca tttttcgaca ttccgacctc aattttgtag gcaaagacgc
     2281 tctgtttcgt cctccgcacg gttggttctt cagatggttg ggcggctacc cggtagatcg
     2341 cagcaagagc aacaacttcg tagattcggt ggtggacatt ttcaaccgcg aaaaggaatt
     2401 ccgcctcacc ttagcgccgg agggcacccg caaaaaagtg gacaaactca aaaccgggtt
     2461 ttattacatc gccgtgggcg ccaaagtacc catcgttttg gtaaaattcg actacggcaa
     2521 caaatgcgtg gccatcgccg agccattcta ccccaccggc gatgccgagg ccgatatgga
     2581 gttcatttcc aatcatttca gaggcgtgcg gggctaccat ccggagctta gtttctgagt
     2641 taccggtgat ctttaaagtc atcaattacc cggcgctctt ttttggtagg acggccggcg
     2701 cctttttcgc ggtattcagc accggccttc cccacaaacc aatctttgta tttgttcagt
     2761 tcttctgccg gagtgaggtc ttcatagcaa gcctgcgcaa tgggcgctcc cacgcgtttt
     2821 tcgatgagat tcagcacttt gtattgcatg ttgaacccct cttttttcac ttccacctga
     2881 tcgcctacct gaatgagttg agatgatttg gcactgatgc cgttcaactt caccttaccc
     2941 tctttgcagg cgtcggtggc gatggtacgc gacttgaaaa tgcgcacact ccacagccat
     3001 ttgtctattc tgaccttttc catgatctac tttttagcaa cgatggccag cgtgatgccc
     3061 atttccgggc gaatgaggtc gtcgccgagc gaatcctcac tgcggtaaaa catgccccag
     3121 tctttgcgat tgatataaaa cttgggcgtc acggcgctga acatgccgtt ggccgcgctt
     3181 actttggcag gaaaactcac acttttggtc gttccgcgca aggtcaggtt gccggttata
     3241 ttgatactgt ccgccgtaat agcccgtgac tgcgtgattt caaaaacagc ctgcggatat
     3301 ttggccgcgt cgaaaaagtc ggcattcaac aggtggcggg tgagcttggc attgcccatg
     3361 tcgccggcgg gcatgtcttc cactttgagg gaagtcatgt caatgacaaa tttgccggca
     3421 acaggcaggt tgccttgcgc attgacgacg cccgaagcga tgcgcaaaga gccattgtgc
     3481 gccgattggc cgaggctcag cccttcatag ccttcccaca gcaggnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnntccagcg tgtaggcatc tgtgccggca atggcagcgg catcaggtgt
     3601 ggcggcggca tctccggttt cggcagtggg gcgtttttcg ttgttgcagg cccaagccaa
     3661 tccggcgatg agcaaaagcg cagtgatgtt tttcataaag tcgggtattt gaaagttatt
     3721 agttacaaac gcatccaata actcctttgc gatgctgttt gtttgaggct tcccacaaaa
     3781 acaaaagccg gccccttgcg gaaccggctc ctgtttgttt atagaacgtg gaaagtttta
     3841 cggac
//
LOCUS       DAORLF010000445         3808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13115_length_3808_cov_1.911271, whole genome shotgun sequence.
ACCESSION   DAORLF010000445 DAORLF010000000
VERSION     DAORLF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13115_length_3808_cov_1.911271"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PK198_23575"
     CDS             complement(<1..928)
                     /locus_tag="PK198_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF41803.1"
                     /translation="MSNAIFQVPKAVNEPVLSYKPGSSERATLKATLAELKKTVADIP
                     MFIGGKRVSTGDKVAIHPPHELSHTLGYYHQGGAEHVQMAIDAALQAKPAWEAMPWEE
                     RAAIFLKAADLLAGPYRAKMNAATMLCQSKNVFQAEIDCVAELCDFYRFNAEFMTDIY
                     RQQPISSPGIWNRMEYRPLEGFVFALTPFNFTSIAGNLPAAPAVMGNTVVWKPSNTQI
                     YSAAVIMEILEEAGLPAGVVNLVYASGPVAGEVIFNHSDFAGIHFTGGTGTFQQIWAT
                     IGQNIHKYRSYPRIVGETGGKDFVMTHASADPL"
     gene            complement(1103..1360)
                     /locus_tag="PK198_23580"
     CDS             complement(1103..1360)
                     /locus_tag="PK198_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41804.1"
                     /translation="MNIRKIKLALTVGLMNLDPQGNALQLVRDLVTELKEAGSFLGLS
                     RVRANDISETYALQFERCTIALDLVSNPKTQSHSVQGFRLQ"
     gene            complement(1583..3028)
                     /gene="ftsZ"
                     /locus_tag="PK198_23585"
     CDS             complement(1583..3028)
                     /gene="ftsZ"
                     /locus_tag="PK198_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRF41805.1"
                     /translation="MLFDLPKVESPIIKVIGVGGGGSNAVNHMYKQGIIGVDFAICNT
                     DSQAMNLSPVPTRIHLGPNLTEGRGAGSKPNIGKLACEESIEDVRRYLDNNCRMLFIT
                     AGMGGGTGTGAAPIIAKTAQEMDILTVGIVTLPFTFEGRRRTDQGVDGLAELKKYVDT
                     IIVVSNDKLRQIYGNLSLSDAFSQADNILTTAAKGIAEIITVPGYVNVDFEDVNTVMR
                     NSGVAIMGTASVGGDDRARRAVDEALHSPLLEDNDIRGAQHILLNISSGSREVTMDEI
                     FEITEFVQEEAGYGTDLIWGNCYDETLGDKLSVTVIATGFNHAPKNAARKTQVSDKIV
                     ISLDDDDRTPPAAQPNKTLRDIGLDDSSISPSVGRTIEFDDLRPNRNPRPGGQQEPYA
                     QHVRDLQQEERSRQLELEQKRREQLRNLNVKLNTPKNVIDLENEPAYMRRGVNLDDLP
                     HSEEHSMSKWSISDDEPQIRENNSFLHNKVD"
     assembly_gap    3283..3432
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3433..>3808)
                     /locus_tag="PK198_23590"
     CDS             complement(<3433..>3808)
                     /locus_tag="PK198_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRF41806.1"
                     /translation="GGTTDITIFHDGIIRHTAVIPFGGNVITRDIKEGCNVMVQQAEK
                     LKVKFGSALSEEVYDNRIITIPGLKGRDHKEISEKNLSLIIQARAEEILDYVMWEIKR
                     SGYERKLIGGLALTGGGALLNHF"
BASE COUNT          725 a          923 c         1098 g          912 t
ORIGIN      
        1 cgagcggatc ggcagaagca tgagtcatca cgaaatcctt gccgccggtc tcgcccacga
       61 tgcgcgggta agagcggtat ttgtggatgt tttggccgat ggtggcccag atttgctgaa
      121 aagtgccggt gccgccggtg aagtgaatgc ccgcaaaatc ggagtgatta aaaatgacct
      181 cccctgctac cgggccgctt gcatacacca aattgacgac gcctgcggga agtccggcct
      241 cctccaatat ttccatgatg acggcagcgg aataaatctg cgtgttggac ggcttccaca
      301 ccaccgtatt gcccatgacg gcgggcgctg cgggcaggtt gccggcgatg gaggtgaagt
      361 tgaagggcgt gagcgcaaaa acgaatccct ctaagggcct gtattccatg cggttccaga
      421 tgcccggaga actgatgggc tgctgccggt agatgtcggt cataaattcc gcattgaagc
      481 ggtagaaatc gcacaactcg gccacacagt caatttctgc ctgaaacaca tttttcgatt
      541 gacaaagcat ggtggccgcg ttcattttgg cgcggtacgg tcctgcgagc aagtcggcgg
      601 ctttgaggaa aatagcagcg cgttcttccc agggcatggc ttcccaggcg ggcttggcct
      661 gcaatgccgc gtctatggcc atttgaacat gctcggcgcc gccctggtga taatagccga
      721 gggtgtggct caactcgtgc ggcggatgaa tggccacttt gtcgccggta ctgacgcgtt
      781 tgccaccgat gaacatcgga atatcggcta ccgtcttttt gagttcggca agtgtggctt
      841 tgagcgttgc gcgttcggaa gaacctggtt tatagcttaa tacgggttcg ttcaccgctt
      901 tgggaacctg aaatattgcg ttggacatga tggttttatt tggttggccg caaaggtaag
      961 gcaagacgtc atatttgggg tagaaatgtg gccactaagt tccattccgc ccggcgattc
     1021 ctcttccaac aacaacggcg ccccgtgatg gaagcgccgc tgcaaagagg ttaagatata
     1081 tagcaaatca gtcgttcgtt atttactgga ggcggaagcc ttgtacggag tggctctgcg
     1141 ttttcgggtt ggacaccaga tcgagggcga tggtgcagcg ctcgaactgg agggcgtacg
     1201 tctcgctgat gtcgttggcc ctgaccctgc tcagtcccag aaacgaaccc gcttctttga
     1261 gttcggtgac gagatcgcgc accaattgga gcgcattgcc ctgcgggtcg agattcataa
     1321 gacctacggt gagtgccagt ttgatttttc tgatgttcat gacgattggt ttttcatggg
     1381 tggtaaaaat gattgtcgtt tgtatgtgtc ttaaactggg aaaaagtacc ctttgttgcg
     1441 gaaaaaaaat taaggtggtg ttaaagaaaa caggagtcgg tttatggaca aaaaaaggag
     1501 cgacgctccc ctcgggaggc cgctccgtaa aataaaaaat actttgtaac gaactaacga
     1561 ttcccagagg tagggaatcg cgtcaatcca ccttattgtg taggaatgaa ttgttttctc
     1621 tgatttgcgg ctcgtcgtcg gagatgctcc acttcgacat ggagtgctct tcggagtgcg
     1681 gcaaatcgtc caaattgacg ccccggcgca tgtaggccgg ttcgttttcc aggtcaatca
     1741 cgtttttggg cgtgttgagc tttacgttga ggttgcgcag ttgttcgcgg cgcttttgct
     1801 ccaactccaa ctggcggctg cgctcttcct gctgcaaatc gcggacgtgc tgcgcataag
     1861 gctcctgttg gcctcccgga cgcgggttgc gattggggcg caggtcgtca aactcgatgg
     1921 tacggcccac ggaagggctg atcgagctgt cgtccaagcc gatgtcgcgc agcgttttgt
     1981 tgggctgtgc agcgggcggc gtccggtcgt cgtcgtccaa agagatgacg attttgtcgg
     2041 aaacctgcgt tttgcgggcc gcgtttttgg gcgcatggtt gaagcccgta gctatgacgg
     2101 tgacgctgag tttgtcgccc agcgtttcgt cgtagcaatt accccagatg aggtcggtgc
     2161 catagccggc ttcttcctga acgaactcgg tgatttcgaa gatttcgtcc atcgtcacct
     2221 cgcggctgcc ggagctgatg ttgagcagga tgtgctgcgc gccccggatg tcgttgtctt
     2281 cgagcagggg cgagtgcaga gcctcgtcca cagcgcggcg ggcgcggtcg tcgccgccta
     2341 cagatgccgt acccatgatg gccacgccgc tgttgcgcat aacggtgttg acgtcctcga
     2401 agtccacgtt cacatagccg ggcaccgtga tgatttcggc gatgcctttg gcggcggtgg
     2461 tgaggatgtt gtcggcctgc gagaaggcgt cggacagaga gaggttgccg taaatctggc
     2521 ggagcttgtc gttggaaacc acgatgatgg tgtccacata ttttttcagt tcggctaagc
     2581 cgtccacgcc ctgatcggtg cggcggcgtc cttcaaaggt aaaaggcagc gtgacgatgc
     2641 ccaccgtcag gatgtccatt tcctgagcgg ttttggcaat gatgggcgcc gcgccggtgc
     2701 cggtaccgcc acccatgccc gccgtgatga agagcatgcg gcagttgttg tccaaatagc
     2761 gacgcacatc ctcgatggat tcttcgcagg ccaatttgcc gatgttgggc ttcgagccgg
     2821 cgccgcgtcc ttcggtgagg tttggcccca ggtggatgcg ggtgggcacc gggctgagat
     2881 tcatggcctg agagtcggta ttgcagatgg caaaatcaac gccgatgatg ccttgcttat
     2941 acatgtggtt gacggcattg cttccaccgc cgcctacgcc gatgactttt atgatcgggc
     3001 tttctacttt tggcagatcg aataacataa gttttgtgtt ttggtcggac cctgctgttt
     3061 ggttgtgtcc gtcagtgaaa gagaaaaaaa gtatcagtaa gtagttcggg agtttgaccc
     3121 tccgtcagaa atcggtatcg ggttctgcct caaaccattc tttggttttg cggaagatgt
     3181 tgtcgtacca ttttcctgcg ccggaggttt cttccaccgc attgctttcg ggcgtggttt
     3241 ccacttccgc cacagcggcg ggcgtcgtca aaccctgctc cannnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nngaagtgat tgagcaaagc gccgccgccg gtaagggcca atccgccgat
     3481 gagcttgcgc tcgtagcctg aacgcttgat ttcccacatc acatagtcca aaatctcctc
     3541 tgcgcgcgcc tgaatgatga gcgagaggtt tttctctgaa atttctttgt gatcgcgccc
     3601 cttcagcccg ggtatggtga tgattcggtt gtcatacacc tcttcagaga gggcagaacc
     3661 gaatttcact ttgagctttt cggcctgttg caccatcacg ttgcagcctt ccttgatgtc
     3721 gcgggtgatg acattgccgc cgaaaggaat cactgccgta tgtcgaatga tgccgtcgtg
     3781 gaagatggtg atgtcggtag tgccgccg
//
LOCUS       DAORLF010000446         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13126_length_3806_cov_1.835777, whole genome shotgun sequence.
ACCESSION   DAORLF010000446 DAORLF010000000
VERSION     DAORLF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13126_length_3806_cov_1.835777"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..590)
                     /locus_tag="PK198_23595"
     CDS             complement(81..590)
                     /locus_tag="PK198_23595"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRF41807.1"
                     /translation="MILSNFPHVADRAFRPEYEHIEWRQDETGLQRWRDGLTGYPLVD
                     AGMRELASTGYMHNRVRMVTASFLTKHLLIDWRLGEAWFAQKLLDFDLASNSGGWQWA
                     AGCGTDAAPYFRVFNPTAQQEKFDPDLVYIRRWIPEYGTSRYPQPIVEHKMARERCLE
                     VYKAGLGKS"
     gene            complement(611..>1310)
                     /locus_tag="PK198_23600"
     CDS             complement(611..>1310)
                     /locus_tag="PK198_23600"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRF41808.1"
                     /translation="HILDDLSDKKDARVEFIHQHVTQLHSQLEALGSTIRVEHGSPAE
                     VWKRLLDELPVGAVYTNHDYEPYALQRDEEVRRLLAERQIPLHTYKDHVIFERQEVVK
                     DDGLPYTIFTPYSRKWRATLLSRTASSDSRAMPAPPFTSKATPTSSISIALLPCLPLP
                     YPLWNHSVLNAPACPSHPPKWPAASFATTTKPATFPASPALPGSACIFGSAPSASAKK
                     PAMPSRSTTPTSTN"
     assembly_gap    1311..1345
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1346..1441)
                     /locus_tag="PK198_23605"
     CDS             complement(<1346..1441)
                     /locus_tag="PK198_23605"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRF41809.1"
                     /translation="MRPIAIFWHRRDLRLHDNAGLYHALKGDLPVL"
     gene            1574..2332
                     /locus_tag="PK198_23610"
     CDS             1574..2332
                     /locus_tag="PK198_23610"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41810.1"
                     /translation="MKQLIFSFATAAVLLLGACGKPGDSAGVPESLRTFVQNANGQQA
                     MDTLVTLLRENAGPAPELATLLAMVMEGKMQDTLAALSVYQAIVQAYPDNKFGKTASE
                     RIPKGTPALDERIEAMQSKMFDPLKMMADPAVMGTYLHSCMARVLLLPKDAKSASMLN
                     KAAENAYYTQQYPKSLYLYEWLEAAYPDDDKAAQALFMRAFIYDNDLKQYDLAKPLYE
                     LFLQKYPKDDFADDAQVLLKNLGKSPEEMIQELQ"
     gene            complement(2427..2891)
                     /locus_tag="PK198_23615"
     CDS             complement(2427..2891)
                     /locus_tag="PK198_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HRF41811.1"
                     /translation="MFGKVKTWLGIEGVKVELVLPELVRETEGAIEGILRFQTMHTQT
                     VTSVKVKLIERYSRGRGDSKLIDEYQLAEIDLVQDIEVPENEVVELDFSLPFQLVKSE
                     VEAFGSKNFLFKGIAKAAQLSRNAKSIYRVEAEAKVRGMALSPFDKKEVVIK"
     gene            complement(2913..3386)
                     /locus_tag="PK198_23620"
     CDS             complement(2913..3386)
                     /locus_tag="PK198_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41812.1"
                     /translation="MNVHFPDTSRLWIYQSARPFSDAEVPVIREQLQQFATRWVSHQV
                     QMKATGEVLHNRFIVLMADESQVSAGGCSIDSSVNFLKNLQAAHHTDLFNRMVFSYLE
                     GEEVQTVSREDFARLYAEGRINDATPVFDTLVSNKGDFDRGFVKPLGESWHKRMV"
     gene            3483..>3806
                     /locus_tag="PK198_23625"
     CDS             3483..>3806
                     /locus_tag="PK198_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRF41813.1"
                     /translation="MNAQLIAQRLNLPIKKIENTLSLLAEGATIPFIARYRKEATGSL
                     DEVDIAEIQRENRSIEEMEKRRESILHSIEEQGKLSEELRRRIENAVDLNELEDLYLP
                     YKRKRK"
BASE COUNT          866 a          943 c         1114 g          848 t
ORIGIN      
        1 gtatagagct gttagctttg gcgggccatt gcggagataa agctaacagc tctttcgcca
       61 gagcgggcgg ctgccaaaaa tcaggatttg cccaagcccg ccttatacac ttcgaggcac
      121 cgttcgcggg ccattttgtg ctctacgatg ggctgcgggt aacgtgaggt gccgtattcg
      181 ggtatccagc ggcggatgta aacgaggtcg gggtcgaatt tttcctgttg ggcagtggga
      241 ttgaaaaccc ggaaataggg cgctgcatcg gtgccgcagc cggcagccca ttgccagcct
      301 ccgctgttgg aagccaggtc gaaatcgagc agtttttggg caaaccaggc ctcgccgagc
      361 cgccagtcta tgagcaggtg tttggtgaga aaactggccg tgaccatgcg cacgcgattg
      421 tgcatgtagc cggtggaggc cagttcgcgc atgccggcgt ccacgagcgg atagcccgtg
      481 aggccgtcgc gccaacgttg caagccggtt tcgtcttgtc gccactcaat gtgttcatat
      541 tcagggcgaa aggcgcggtc ggcaacgtga gggaaattag ataaaatcat ggcgtaaaaa
      601 tcgcgccata tcagttcgtt gaggtaggtg tcgttgagcg agagggcatg gcgggctttt
      661 tcgcggatgc tgatggtgcc gaaccgaaaa tgcacgccga gccgggaagt gccggcgatg
      721 ccgggaatgt cgcgggtttt gtcgtagttg cgaatgatgc tgcgggccac ttcggcggat
      781 gggaagggca agccggcgcg ttcaaaaccg agtgattcca aagagggtag gggagcggga
      841 ggcagggaag caaagcgatg gaaatactgc tcgttggggt agcttttgag gtaaaaggag
      901 gcgccggcat cgcccggctg tcgctgctgg cggtacggga gagcaatgtg gcacgccatt
      961 tgcggctgta cggggtgaaa atggtgtagg gcagcccgtc gtctttgacc acttcctgtc
     1021 gctcaaagat gacgtggtct ttataggtat ggagcgggat ttgtcgttct gcgagcagtc
     1081 ggcgcacttc ttcgtcgcgt tgcagggcgt agggttcgta gtcgtggttg gtataaacgg
     1141 cgccgacggg cagctcgtcc aacaggcgtt tccacacctc cgccggacta ccatgctcga
     1201 cgcggatggt gctgccgagt gcctctaact ggctgtgcaa ctgtgtgaca tgctgatgaa
     1261 tgaactctac gcgggcatct tttttgtcgg agaggtcgtc cagaatatgg nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnncagca cgggcagatc gcctttgagg gcatggtaga
     1381 ggccggcgtt gtcgtgcagg cggagatcgc ggcggtgcca gaagatggcg attggtcgca
     1441 tgtcggttgg atgtttgggg gtgaaacaga tgggcggggg gaatgttttg gggggaaatt
     1501 tagaaacagc gaacttttac aacgagttta aaacgaatgt aatttacctt cgccccgaca
     1561 aaccaaacgg accatgaaac aacttatttt ttcttttgcg actgcggctg ttctgttgct
     1621 gggcgcctgt ggcaaaccgg gcgactctgc cggagtgccg gaatcgctgc gcactttcgt
     1681 gcaaaatgcc aacggccaac aggctatgga taccttagtg acgctgttgc gtgaaaacgc
     1741 cggccctgcg cccgaattgg ctaccctgct ggcgatggtg atggaaggca aaatgcagga
     1801 caccctcgcg gcgctgtctg tgtatcaggc cattgtgcag gcttatcccg acaacaagtt
     1861 cggcaaaacg gcctctgaac gcatcccgaa aggcacgccg gctttggacg aacgcataga
     1921 ggccatgcag agcaaaatgt tcgatcctct gaaaatgatg gccgaccctg cggtgatggg
     1981 tacttaccta cactcctgta tggcgcgggt gctgctcctg cccaaagacg ccaaaagtgc
     2041 ctccatgttg aacaaggccg ctgaaaatgc ctattacact cagcagtacc caaagtcgct
     2101 gtacctatac gaatggttgg aagcggcata tcccgacgac gacaaggcgg ctcaggcgct
     2161 gttcatgcgg gcctttattt acgataatga cttgaaacag tacgacttgg ccaagccgct
     2221 gtacgagctg tttttgcaaa aataccccaa ggatgatttc gccgacgacg cgcaggtgct
     2281 gctcaaaaac ctgggaaaaa gcccggaaga gatgattcag gagttgcagt aggcggtagg
     2341 cggtgggcag tgcaagcgta gagggttcag taggcggtag gcggtgcaag cgtaaggtgt
     2401 accgcctact gccaaccgcc caccgcctac ttaatcacca cttctttctt atcaaaaggc
     2461 gacaaagcca tgccgcgcac tttggcttcg gcttccacgc gataaatgga tttggcattg
     2521 cgggagagtt gggcggcctt ggcgatgcct ttgaaaagga aattcttgct gccgaaagcc
     2581 tccacctctg atttgaccaa ctggaacggc agcgaaaaat cgagttctac cacttcattt
     2641 tcaggcactt cgatgtcttg tacgaggtca atttcagcca attggtattc atctatcaac
     2701 ttgctgtctc cgcggccgcg cgagtaacgc tctatcagtt ttacctttac ggaagtgacc
     2761 gtttgcgtgt gcatggtctg aaagcggagg atgccctcga tggcgccttc tgtttcccga
     2821 accaactcag gcagaacgag ttctactttg acgccctcaa tgccgagcca ggtttttact
     2881 tttccaaaca tgattgtttt ttttgaaatg gttcagacca tgcgtttgtg ccagctttcg
     2941 cccagcggtt tgacgaagcc ccggtcgaag tcgcctttat tgcttaccaa tgtgtcgaac
     3001 accggcgtag cgtcgttgat gcggccctcg gcgtaaagtc gggcgaaatc ttcgcgggaa
     3061 accgtttgga cttcttcgcc ttccaaatag ctgaatacca ttctattgaa aagatcggta
     3121 tgatgcgcag cctgaagatt ttttaaaaaa ttgaccgagg agtcaatcga acaaccaccg
     3181 gcgctcacct gactttcatc agccatcagt acgataaaac gattgtgcaa aacctcgccg
     3241 gtggccttca tttgcacctg atggctgacc cagcgcgtag caaattgttg caattgctcg
     3301 cggatgacgg gcacctccgc gtcgctgaag gggcgggccg attggtaaat ccagaggcgg
     3361 gatgtatcgg gaaaatgtac gttcatgctg acgacgtttt tttgacaaca aacagaccga
     3421 agccgcgaat ggttggcggg gccgaatcaa gccttatatt tgcgccccat atttctcgac
     3481 caatgaatgc ccaattgatt gcccaacggc tgaatctgcc gattaaaaaa atagaaaata
     3541 ccctctcctt gctggccgag ggcgctacca ttccttttat agcccgctac cgaaaagaag
     3601 ccaccggttc tttggatgaa gtggatattg ctgaaattca gcgcgaaaac cgcagcatcg
     3661 aagaaatgga gaagcgccgc gaaagcatcc tgcactccat tgaggagcag ggaaaactca
     3721 gcgaggaatt gcgccgccgc atcgaaaacg cagttgacct gaatgagttg gaagacctct
     3781 acctgccgta caaacgcaag cgaaaa
//
LOCUS       DAORLF010000447         3787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13205_length_3787_cov_2.199089, whole genome shotgun sequence.
ACCESSION   DAORLF010000447 DAORLF010000000
VERSION     DAORLF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_3787_cov_2.199089"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PK198_23630"
     CDS             complement(<1..143)
                     /locus_tag="PK198_23630"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRF41814.1"
                     /translation="MSGLDVVWLNKFISESGLCSRREADKYIEQGLVFVNGQKAQKGD
                     QVWP"
     gene            255..605
                     /locus_tag="PK198_23635"
     CDS             255..605
                     /locus_tag="PK198_23635"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRF41815.1"
                     /translation="MIIEGAIGDAYGAGFEFADMEFIKNKNQLTHYQQHPLFESIYKK
                     YTDDTQMALAIAELLIEKTAWTPVAIADKFVEVFKRDPRQGYAKGRSRKVDRIFVRPA
                     TSKMGIRLARRSGH"
     gene            511..876
                     /locus_tag="PK198_23640"
     CDS             511..876
                     /locus_tag="PK198_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41816.1"
                     /translation="MPKGDREKLTEYLYDLQQVRWALGWQGEVGIDAVQTVRAVFTVL
                     SEAHSFSEILKKSVDLGGDVDTVASLALALAAVDKNMENDLPRWLYDELENGPFGRDY
                     ILKIDNELFKLKNIVSIQQ"
     assembly_gap    1319..1354
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1355..1691
                     /locus_tag="PK198_23645"
     CDS             <1355..1691
                     /locus_tag="PK198_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41817.1"
                     /translation="LSQSFDDRSSTLFDFFPDDDDKSLEFIDAAEAVEVLFRNNKVPV
                     WIDISVESTFNQHTVFRLLCAGRYSSDEQALYYANNGTGSFGIKSPALPVDFVEGKKF
                     KLHHPPTAH"
     gene            complement(1767..2867)
                     /locus_tag="PK198_23650"
     CDS             complement(1767..2867)
                     /locus_tag="PK198_23650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF41818.1"
                     /translation="MSPSIMNLNNGGVCPQPRVVQEAVERYNRLSNEAPSYYMWQILD
                     QGREPLRAQLAELAGCSKDELAINRNASEALETIIFGLRLQAGDEVVLTKQDYPNMIQ
                     AWKQREKRDGIVLKWISFDFPIEDAALITRRFADAFTPRTKVVHITHVINWMGQVLPA
                     RDIAREARKRGIEVLVDAAHSFAHIDYKIPDLDCDYWGTSLHKWLCAPFGSGLLYVRK
                     EKIRNLYPLFGAPDPESEDIRKFESLGTRSFAIEQAIGQAVHFHRMIGAELKQKRLQY
                     LKNYWAERVQKLPGVQVKTSLRPEFACALALVAVEGKTPAEVQGFLFREFKIHTVAIE
                     WENIKGVRITPNVYTTTDDLDVLVRAFREMVG"
     gene            complement(3131..>3787)
                     /locus_tag="PK198_23655"
     CDS             complement(3131..>3787)
                     /locus_tag="PK198_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRF41819.1"
                     /translation="SHHTFSFAHYHHPERMRFGALRVLNDDTVAAGMGFGTHPHDNME
                     IISIPLEGDLEHKDSMGNVTVIRHGDIQVMSAGTGVRHSEFNKNKDSQVKFLQIWVFP
                     KQRNVTPRYDQISLDPADRVNQLQQILSPSPDDAGVWIHQDAWFHLGRFDEGKGVGYR
                     VHKAGNGVYAFVLSGSFQVNGIALDARDGLGIWDTDKFDLAAVSPGSEILLMEVPMAV
                     "
BASE COUNT          896 a         1007 c          932 g          916 t
ORIGIN      
        1 ggccacacct gatcgccttt ttgcgctttt tggccattga caaaaaccaa gccctgctcg
       61 atgtacttgt cggcctcccg acgcgaacaa agcccgcttt cgctgatgaa tttattgagc
      121 caaacgacat ccaaacctga catgactatt ttttgcgcaa ggtagggttt ttggagaatc
      181 gggaggcggg ttttgtgtaa acctcttggg gcggaatggt gtatttatga tctaatgaat
      241 aaaacctgaa cagtatgatc atagagggcg ccatcggtga tgcttatgga gcaggctttg
      301 agtttgcaga catggaattt ataaaaaata aaaaccaact gactcattac caacaacatc
      361 cgttgtttga atccatttat aaaaaataca cagacgatac gcaaatggcg ctggccattg
      421 ccgaactgtt gattgaaaaa acagcgtgga cgccggtggc cattgccgat aaatttgtgg
      481 aggtattcaa acgagacccc agacaaggct atgccaaagg gagatcgcga aaagttgacc
      541 gaatatttgt acgacctgca acaagtaaga tgggcattag gctggcaagg cgaagtgggc
      601 attgatgcgg tacagacggt tcgggctgtt tttacggtgc tgtcggaggc gcattctttt
      661 tcagaaatac tcaaaaaaag tgttgatctg ggtggagatg tggatacagt tgcctccctc
      721 gcgctggcgc tggctgctgt tgacaaaaac atggaaaacg acctgccccg ctggctctat
      781 gacgaacttg aaaacgggcc attcgggcga gactacattt tgaagattga taatgagttg
      841 tttaagttga aaaatattgt ctctatccaa cagtaatact ggtatgtaca atttcaaaat
      901 catcgtttgg actatctttg tatcagtcct ggtttatgct cgaattgtca ttgtatctgg
      961 ggtgaatcca ttggctacag ggttgattaa ctttgctctt gttgcgttcc ttatatggat
     1021 tatatatgcg atttccaaaa cctcattttt aaaatttaaa atattctatg ccacagtttt
     1081 tgttgtattt cttggaactg cgctattgct tgcgaatgtc aaaatgcgtc aattaactaa
     1141 ctttcaggac aagacttaca ttctggagcc gcagtagaag cgtcggcgta gcagatacaa
     1201 acattcaaaa actcaaccga tgactaatga agccttcttc gaactgttaa ttaccgcctc
     1261 gcaacttgcg tacgattttg cgaaagattt tgtgacggat gaactacctg ccaatttcnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctcag tcagtctttt gatgatcgaa
     1381 gctccactct ttttgatttc tttccagatg atgatgacaa aagccttgaa tttatagatg
     1441 cagcagaagc ggtcgaggtg cttttcagaa acaataaagt tcctgtgtgg atagacattt
     1501 cagtagaaag caccttcaat cagcataccg tattcaggct gctttgtgcc ggcagatatt
     1561 cgagtgacga acaagcgttg tattatgcca ataacggaac aggctctttt ggaattaaaa
     1621 gcccggccct gcccgtagat tttgttgagg gcaaaaaatt caagctccat catcccccca
     1681 ccgcccattg agtaccgcct actgtccccc catcgcccac tctacgctta gcaccccgcg
     1741 ccacaggcgc ggccaccgcc caccacctac cccaccatct cccgaaacgc ccgcaccagc
     1801 acatccagat catccgttgt ggtataaaca ttcggcgtga tgcgcacccc cttgatgttc
     1861 tcccactcga tggccaccgt atgtattttg aactcccgga acagaaaacc ctgcacctcc
     1921 gccggcgtct tgccctccac tgctaccagc gccagcgcac aggcaaactc aggccgcagc
     1981 gaagtcttca cctgcacccc cggcaatttt tgcacccgtt ccgcccagta gtttttcagg
     2041 tattgcaagc gcttttgctt cagttcggcg ccgatcatgc gatgaaaatg caccgcctgc
     2101 ccgatggcct gctcgatggc aaaagagcgg gtgcccaaac tctcgaattt gcggatgtct
     2161 tcactctcag gatcgggcgc gccgaagagc ggatacaggt tgcggatttt ctccttgcgc
     2221 acatacagca gcccgctgcc gaacggcgcg cacagccatt tgtgcaggct cgtgccccag
     2281 tagtcgcagt ccagatcggg gattttgtag tcaatatgcg caaaactgtg ggccgcatcc
     2341 accagcacct ctatgccgcg tttgcgagcc tcgcgggcta tatcgcgggc cggcaacacc
     2401 tgccccatcc agttgataac gtgcgtgata tgcaccacct tggtgcgcgg cgtgaaggca
     2461 tccgcaaagc ggcgcgtgat gagcgcggca tcctctatcg ggaaatcgaa tgaaatccat
     2521 ttcagcacaa tcccgtctcg tttttcccgc tgtttccagg cctgtatcat gttcggatag
     2581 tcctgtttcg tcagcaccac ctcgtctccg gcctgtaggc gcagcccgaa aatgatggtt
     2641 tccagcgcct ctgaagcatt gcggttgatg gccagctcgt ctttagagca gcccgccaac
     2701 tcggccaact gagcgcgcag cggctctcgg ccctggtcca gtatctgcca catatagtac
     2761 gagggcgcct cgttgctcag ccggttgtag cgctccaccg cctcctgcac cacccggggt
     2821 tgcgggcaca cgccgccgtt gttcaggttc ataatgctcg gcgacacggt gtaagcctgt
     2881 ttgatctggt accaaaaacg gtcacgagtt cgtcggcagg gcgcaggcga ctttcgtcca
     2941 ccaagcgaag cagggcacgg ccttgcggcg tttccataaa aggcgcagcc acagcgaggc
     3001 cggcaaaacg agaaaggaaa gtacggcgat tttgcatggt gatgaatgtt ttaaacaaag
     3061 atagtacaat cacccaaaaa cgcgcgtatc tgttctaccc gcgtatgaaa aagctaaaac
     3121 aaaacagact ctacaccgcc atcggcacct ccatcagtag tatttccgaa ccgggcgata
     3181 ctgctgccag atcaaacttg tcggtatccc aaatgccgag tccgtcgcgg gcgtccagcg
     3241 caataccgtt cacctgaaag ctccccgaaa gcacaaacgc atacacgcca ttgccggctt
     3301 tgtggacgcg gtacccgacg cctttgccct catcaaaccg gcccagatga aaccaggcat
     3361 cctgatgaat ccaaaccccg gcatcatcag gcgaaggcga gaggatttgc tgcaattgat
     3421 tgacgcggtc ggccggatca aggctgatct gatcgtagcg gggcgtcacg ttgcgctgtt
     3481 tggggaacac ccagatttgc aggaatttga cttggctgtc cttattttta ttaaactcgc
     3541 tgtggcgtac gcccgtgccg gcgctcatca cctgtatgtc gccgtggcga atgacggtca
     3601 cattgcccat gctgtctttg tgctccaggt cgccctccag cggtatgctg atgatctcca
     3661 tgttgtcgtg cgggtgcgtg ccgaatccca tgccggccgc caccgtgtcg tcattgagca
     3721 cccggagtgc gccgaatctc atgcgttcgg ggtggtggta gtgtgcaaaa ctgaaggtgt
     3781 ggtgcga
//
LOCUS       DAORLF010000448         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13209_length_3786_cov_1.777540, whole genome shotgun sequence.
ACCESSION   DAORLF010000448 DAORLF010000000
VERSION     DAORLF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13209_length_3786_cov_1.777540"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>376
                     /locus_tag="PK198_23660"
     CDS             <1..>376
                     /locus_tag="PK198_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41820.1"
                     /translation="VITPFGGSSFTWSVSSLGNIQSGQGTNEINVVWAGQFSSQPQWV
                     AVEYENCYLGCGGRDTIWVDIHPEFFVSGRIELCLNESGTYAARRVIFGNNVLCNWEA
                     VAADGSVVWASSTAVATTLVAWN"
     assembly_gap    377..386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <387..>769
                     /locus_tag="PK198_23665"
     CDS             <387..>769
                     /locus_tag="PK198_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41821.1"
                     /translation="GYFRLIARPASPNDYCIPAYEVTVHVLELPPALSGIAGASEVCP
                     ELPYTYQALGLLPGHTAAWSWSGISGSAPARGNPVNAVWSDTQPLSHSVQVRQVSARG
                     CISPAIALPISALPELSLSGVGEAC"
     assembly_gap    770..788
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <789..>2727
                     /locus_tag="PK198_23670"
     CDS             <789..>2727
                     /locus_tag="PK198_23670"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41822.1"
                     /translation="RSLYATDALEQVEYNWSVSPAGAGTVYRVGNAHEIEIHWHTEGL
                     AEVHLSVCGQNLSVPVLIRPKPEPHVNVPAFLCAGETITVQTTAPFASYIWKDAADHL
                     VSDSARAVVTAGYYQVEVKNDFNCRSTALFDIRNRPVPEVSLSTPNNTGICPGDPPTI
                     LHTTESGITLTYAWFRNGNPIAGAVGSTYPTTETGLYFVKATDGAGCSAFSNTLTLFH
                     YCDPISGGGGFPGGCPGGSCGNPGLNCPINTLINIGSQPSSSCNTFHYTNQSTNVIPG
                     SLQWNFGDPGSGGNNISELENPTHSFSRAGYFGIYLAGIVETPAPVICYAVRVDTVPL
                     AARFFYESACAGEPVLLTDASTFLPGQTIVAYHWNFGDPASGPANTDDSANTEHVFSS
                     PGNFVVTLTVTAESGCVSTFADTVEVFGPPAMAFFDEPTTLCAATALPFDAQLSNDVV
                     NFYWNFGDPASGNANASQALQPFHRYESPGLHSITLDAVNIYGCTRSFQRNITVEVND
                     LSGDIAFQSPICEGDSTLLSAPPGGAQWLWSNGEISESIWAHQSGVYKVNLTGATGCA
                     YEPPPAAIQVNPAPRAPIRAIEYNEEGFPRTYTYDSLSVCVGENVFLEVGAQSGHTYR
                     WFTDLPGPYIEFSDSRDNRLP"
     assembly_gap    2728..2781
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2782..>3112
                     /locus_tag="PK198_23675"
     CDS             <2782..>3112
                     /locus_tag="PK198_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41823.1"
                     /translation="YTEHFRVIVRPLPLMPATTASPSGPICEGTPVHFAVTNIQPGVM
                     YDWSSGASGATWTTSRAGAWQVRAANEWGCVSTGSVHHILPSPAINLIPSGCHIRCRP
                     DTLCFPPV"
     assembly_gap    3113..3123
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3124..>3786
                     /locus_tag="PK198_23680"
     CDS             <3124..>3786
                     /locus_tag="PK198_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRF41824.1"
                     /translation="IVSYQWMFNGNEQGPASASVPQIVITESGQYSMRMTTLQGCTSD
                     SHPLTLDLFDGSGDLRGFVYWDRNNNGVLDPTDSLLSNIPFMLLSGNMPLDTILSQND
                     GQYSFVNMPQASYSIQLDAAALPDGLLPDLTRIDTILAGCDVEVLLYWRVICPQTASS
                     VALQACPGSTIDYNGNMLPPGTTTDFTLTNAAGCDSIVTVTVAALPTSASSVALQACP
                     GST"
BASE COUNT          788 a         1062 c          967 g          875 t
ORIGIN      
        1 gtcatcacgc cattcggcgg aagttccttt acctggtctg tttcgtcatt gggcaatata
       61 caatcagggc agggtactaa cgaaatcaat gtggtttggg ccgggcagtt cagctctcag
      121 ccacaatggg tagcggtgga gtatgaaaac tgttatttag gctgcggcgg cagggataca
      181 atatgggtgg acatacatcc ggaatttttt gtctccggaa gaatagagct gtgcctgaat
      241 gagtccggca cctacgcggc gcggcgcgtc attttcggca acaatgtgtt gtgcaattgg
      301 gaagcagtag ctgccgacgg gtcggttgtt tgggcctcca gtacagccgt agcaacaact
      361 ttggtggcat ggaattnnnn nnnnnncggg ctatttcaga ctgattgccc ggccggcctc
      421 gcccaatgat tattgcatcc ccgcatacga ggtgacggtg catgtattgg aactgcctcc
      481 ggcgctctcc ggcatagcag gagcaagcga ggtgtgcccg gaacttcctt atacatatca
      541 ggcgctgggg ctgttgcctg gccacacggc agcatggtct tggagcggta tcagcggcag
      601 cgcgccggcg cgaggcaatc cggtgaatgc cgtatggagc gatacacaac cgttgagcca
      661 cagcgtgcag gtcaggcagg taagcgcccg tggttgtatc tctcctgcaa ttgccttgcc
      721 gatatctgca ctgccggagt tgtcgctgtc cggcgtcggc gaagcttgtn nnnnnnnnnn
      781 nnnnnnnnag cgttcactgt atgccaccga tgcgttggag caagtggagt acaattggtc
      841 ggtttctccg gcaggagccg gcacagtgta tcgggtaggc aatgcgcacg agatagagat
      901 acattggcac accgaggggc ttgcggaagt acacttgtcg gtttgtggcc aaaatctgtc
      961 tgtgccggtc cttattcgcc ccaaacccga acctcatgtc aatgttcctg ccttcctctg
     1021 cgccggcgaa accataacgg tgcaaactac ggcccctttt gcctcttata tctggaaaga
     1081 cgcagccgac cacctggttt ccgactccgc ccgtgcagta gtgacggcag gatactatca
     1141 ggtagaggtt aaaaacgatt tcaactgccg gagtaccgct ctttttgaca taagaaatcg
     1201 ccctgtgccg gaggtgtcgc tctctacccc caataataca ggcatttgcc ccggcgatcc
     1261 gcccaccatt ttgcatacca cagagtcggg tataacattg acctatgcct ggtttcgcaa
     1321 cggaaacccc atagcgggcg ctgtcggctc tacatatccc accacagaaa ccggcttata
     1381 ttttgtaaaa gcaaccgatg gcgcaggttg cagcgccttc tccaacacgc tgaccttatt
     1441 tcattattgc gatccgatca gcggtggcgg cggctttccc ggtggatgcc cgggcgggtc
     1501 atgcggcaac ccgggactaa attgccccat caatacattg ataaatattg gttcacagcc
     1561 ttcatcaagt tgcaatacct tccattatac caaccaatca acaaatgtga ttccaggttc
     1621 attgcaatgg aattttggag accccggatc aggtggcaac aatatttccg aacttgaaaa
     1681 ccctactcac tcctttagca gggcaggcta tttcggcata tacctggcgg ggattgtaga
     1741 gacgcccgcc cctgtaattt gctatgccgt ccgcgtggac acagtgccgt tggctgcccg
     1801 atttttttat gaatctgcct gcgccggcga acccgtatta ctgacggatg cgagtacatt
     1861 ccttcccggg caaaccattg ttgcttacca ctggaacttc ggcgaccccg cctccggacc
     1921 ggccaatacc gacgacagcg ccaatacaga gcacgtcttt tcctctccgg gcaacttcgt
     1981 cgtaacgctg accgtgaccg ccgagagcgg atgcgtttct acatttgccg atacggtaga
     2041 ggtattcggc ccgcccgcaa tggccttttt cgatgagccg acgacgcttt gcgcagcaac
     2101 tgcgctgcct ttcgatgccc aactcagtaa tgatgtggtg aatttttact ggaatttcgg
     2161 cgaccctgct tccggcaatg caaatgcttc gcaagcttta cagccttttc atcgctatga
     2221 gtctccggga ttgcattcca taactttgga tgcggtcaac atttacggct gtacccgttc
     2281 cttccaacgc aatatcacgg ttgaggtgaa cgacctcagc ggcgatatcg ccttccaatc
     2341 gcccatctgc gaaggcgaca gcaccctgct ctccgcgcct ccgggcggag cgcaatggct
     2401 ttggagcaac ggagaaattt cggaaagcat ttgggcgcat caatccgggg tttataaagt
     2461 aaatctgacc ggcgcaacag ggtgcgctta cgagccgcct ccggccgcca tacaagtgaa
     2521 tccggcgccg cgagcgccca tacgagccat agagtacaac gaggaaggtt ttccaagaac
     2581 ttatacctac gattcattat cggtgtgcgt cggcgaaaat gtgtttttag aggtcggcgc
     2641 gcaatccgga cacacttatc gctggttcac cgacctcccg gggccgtata tagaattttc
     2701 ggacagccgc gacaatcgcc tgcccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nttacacgga acattttcgc gtcatcgtca ggcccttgcc
     2821 gcttatgccc gcaacaaccg cttccccgtc cggccccatt tgcgaaggca ctcccgttca
     2881 ttttgccgta accaatatac agccgggcgt catgtatgat tggtcttcag gagcatcggg
     2941 agccacatgg accacctcgc gcgcgggcgc ctggcaggtc agagccgcca acgagtgggg
     3001 ctgcgtgagt acgggcagcg tgcatcatat cctccccagc ccggccatca atcttattcc
     3061 ttccggctgc cacattcgtt gccgtcccga tacgctttgt ttccctcccg tgnnnnnnnn
     3121 nnntatcgtt tcttatcaat ggatgttcaa cggcaacgaa caaggtccgg cttccgcttc
     3181 cgtgccacag atcgttatta cagaaagtgg ccaatactcc atgcgaatga ctactttaca
     3241 gggttgcacg tcggattcgc acccgctcac gctcgacctc tttgacggat caggagactt
     3301 gcggggcttt gtatattggg acagaaacaa caacggggta ctcgacccca cagattcctt
     3361 gctgagcaat atccctttca tgctcctctc cggcaatatg ccattagata cgatcttgtc
     3421 tcaaaatgac ggacagtata gttttgtcaa tatgccgcaa gcctcttaca gtattcagtt
     3481 agacgccgca gccttgcctg atggcctcct gcccgatctg actcgcatag ataccattct
     3541 ggcgggctgc gatgtagagg tattgcttta ctggcgggtg atctgccctc aaactgcttc
     3601 ctccgttgcg ctgcaagcct gtcccggcag tacgattgat tacaacggca acatgctccc
     3661 gccaggaacc acaacggatt tcaccctgac gaatgcggcg gggtgcgatt ccatcgtgac
     3721 cgtaaccgtg gcggcattgc ccacaagcgc ttcctccgtt gcgctgcaag cctgtcccgg
     3781 cagtac
//
LOCUS       DAORLF010000449         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13264_length_3773_cov_3.335126, whole genome shotgun sequence.
ACCESSION   DAORLF010000449 DAORLF010000000
VERSION     DAORLF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13264_length_3773_cov_3.335126"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK198_23685"
     CDS             complement(<1..394)
                     /locus_tag="PK198_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRF41825.1"
                     /translation="MGLLKKGVIFAMCGIAVLTACDPDKPEPLPDIRFETPANFPAPL
                     YDLAANPVTPAGFALGRDLFYDGILSRDGSISCGFCHQQTAGFTQHGHDLSHGIDDRL
                     TMRNALPIQNLAWEREFFWDGGVFHLDLF"
     gene            complement(471..1283)
                     /locus_tag="PK198_23690"
     CDS             complement(471..1283)
                     /locus_tag="PK198_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41826.1"
                     /translation="MQLNHFKSILFLALIAVFFAGCNKDEFKAGDKGSIVIEFDNVAG
                     NQDLQLNTGNYTNAAGESFNISLFQYYISNIKLQTEDGDEYVVPQKESYFLIQEHKDH
                     THSVVLNDVPAGNYNSVSFIIGVDSLRNTMAPSERTGVLDVAAYTGDENMYWAWNSGY
                     IFVKTEGISPQAPVGTDGQRKFRYHVGGYGGYSSATINNIKSKSLSFGSEVAKVRKDA
                     TPEVHLKVDILNLFNGSPNISVAANPTVHFAPFSVNIANNYVNMFKFDHVHN"
     gene            complement(1323..2237)
                     /locus_tag="PK198_23695"
     CDS             complement(1323..2237)
                     /locus_tag="PK198_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41827.1"
                     /translation="MKTVLAAALLWVLASGQVLACDVCGCAVGGSYLGILPQFRRHFA
                     GVRFQYRTFDSEHPNLFGGGIESVSREQYLTAETWGRWTPLHNVQVFGFVPYHHFSRT
                     EDGSLTRASGLGDASVVANWVVFNTGDSTRRAWKHALQLGGGVKLPTGNARLLQSNGE
                     AMPRNMQPGSGSVDFPINAMYTLRRGAWGMTAEGNYRINTPDAEGYRFGHRAGLASRI
                     FYWKSMKHMTMLPGAGIFWEHTSSDRLHGTAQDFTGGHSLWAGVGMDWFMRRWAFSIL
                     YQHPLAAHIGEGYIQPGPRASAGISYLF"
     gene            complement(2560..2943)
                     /locus_tag="PK198_23700"
     CDS             complement(2560..2943)
                     /locus_tag="PK198_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41828.1"
                     /translation="MRSFISISLVFLFAWQLLLKAGVLMWYGANRTYIAAELCENRDK
                     PEMQCNGQCVLAKRMKAAEQERRDADQRPLRIMEKFEFSHYIAAAEDIFSGMFETDLS
                     RRIVWASYLEPHSNYFSNFFHPPEL"
     assembly_gap    3183..3192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3193..3750)
                     /locus_tag="PK198_23705"
     CDS             complement(<3193..3750)
                     /locus_tag="PK198_23705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF41829.1"
                     /translation="MQIHRIPFSGGELRYVETGLQTDSTPLILFVHGAPGSGNNFYKY
                     LSDSLLTAKYRLISIDRPGYGYSNYGQSFRVQAEAVKAVLDRYRPSKTLLVGHSFGGP
                     VIAQVALDYPAQVQALLMLAPVNDPDSEPVPWYAGLARWRLTRWMLSKAWQVSGDEKF
                     AHVEELRRLQPRWQELRIPVTHWHGA"
BASE COUNT          928 a          945 c         1029 g          861 t
ORIGIN      
        1 caaaaaggtc taagtgaaaa acgccgccgt cccaaaaaaa ctcgcgctcc caggccaggt
       61 tttgaatggg cagggcattt ctcatggtga gccggtcgtc aatgccgtgg ctgagatcgt
      121 ggccgtgctg agtaaaaccg gcagtttgct gatggcaaaa accgcaactg atgctgccgt
      181 cgcggctcag gatgccgtcg taaaacaaat ccctgcccag tgcgaatccc gccggcgtta
      241 ccgggttggc ggccagatca tatagcggcg cggggaaatt ggccggcgtt tcaaaacgaa
      301 tgtcgggcag cggctccggc ttgtcggggt cacaggcagt gagaacagct atgccgcaca
      361 tcgcaaaaat tacccctttt ttgagaaggc ccatgtggaa aatatgttgt aaagttgtcc
      421 ggcagttttg aggccgccgg acaaccgaat tgatggtata atggaaaatc ttagttgtga
      481 acgtgatcaa acttgaacat attcacatag ttgttggcga tgtttactga aaatggcgca
      541 aagtgaaccg tcggattggc ggccacgctg atgttggggc tgccgttgaa caggttcagg
      601 atgtccactt tcaggtgtac ctccggcgtc gcgtctttgc gcactttggc cacttcgctg
      661 ccaaaagaga gcgactttga cttgatgttg ttgatggtgg cgctgctgta gccgccgtaa
      721 ccgcctacat ggtagcggaa tttgcgctgc ccgtcggtgc ctaccggcgc ctgcggtgaa
      781 atgccttctg tttttacaaa aatatagccg ctgttccagg cccagtacat attttcatct
      841 cctgtatagg ctgccacgtc caatactccg gtgcgctcgg aaggcgccat cgtattgcgc
      901 aggctgtcca cgccgatgat gaaactcaca gagttgtagt tgccggccgg aacgtcgttg
      961 agtactacag aatgcgtgtg gtctttgtgt tcctgaatga gaaaatagct ctctttttga
     1021 ggcactacat actcatcgcc gtcttccgtt tgaagcttga tgttgctgat atagtactga
     1081 aacagagaga tgttgaaaga ctcgccggcg gcattggtat agttgccggt attgagttgc
     1141 aggtcctgat tgccggccac attgtcaaac tcaatgacga tgctgccctt gtctccggct
     1201 ttgaactcgt ctttgttaca tccggcaaaa aatacagcga tcaatgccag aaaaaggatg
     1261 gatttaaaat gattcaattg catgttgaat gttgtttttg atggttgaaa aaaagttgtg
     1321 atttaaaaga gatagctgat acctgccgat gctcttggcc ccggctgaat gtacccttcg
     1381 ccgatatgcg cagccaaagg gtgctgatac aatatcgaaa atgcccaccg ccgcatgaac
     1441 caatccatgc cgacgccggc ccaaaggctg tgtcctccgg taaaatcctg tgcagtcccg
     1501 tgcagcctgt ctgaggaggt gtgctcccag aaaatgccgg cgccaggcag catcgtcatg
     1561 tgcttcatag atttccagta aaaaatgcgg gaagccaagc cggcacgatg accgaaacga
     1621 tagccttctg catccggcgt gttgatgcgg taattgccct ccgccgtcat gccccaggct
     1681 ccgcgccgga gggtgtacat ggcgttgatg gggaaatcaa ccgagccact gccgggctgc
     1741 atattgcggg gcatggcctc tccattggat tgaagcaggc gcgcgtttcc ggtaggcaac
     1801 ttgacgccgc ctcccaattg caaagcgtgt ttccatgcgc ggcgggtgct gtcgcctgta
     1861 ttaaaaacaa cccaattggc taccacagag gcgtctccga ggccggaggc gcgcgtcagg
     1921 ctgccgtctt cggtacgcga aaagtgatgg tacggcacaa agccgaatac ctgtacgttg
     1981 tgaagcggcg tccagcgccc ccaggtttct gccgtaaggt attgttccct agagaccgat
     2041 tctatgcctc ctccgaaaag attcgggtgc tcggaatcga aagtccggta ctggaatctg
     2101 accccggcga aatgcctcct gaattggggc aaaataccga ggtaacttcc tccgacggca
     2161 catccacaaa cgtcacaggc aaggacctgg ccggaagcaa gcacccaaag cagggctgct
     2221 gccagtacag ttttcatgtc tgtaaattgt gatggctatt attcagcggc tttcagaaag
     2281 gggcgataaa aaaataaagt gatcattttc gccaaaggat tagataagta aacgtttcat
     2341 tttaagtgtc ttatctaatc ctttggccta aagaaaatga acatttattt ttgccccact
     2401 acaaagctgg gcgcagcaag actgatttcc ccaaaagaaa tcatgcaacg cgggcgcctg
     2461 acttttggaa cagactgaat aacggcaatg aataaaatgg gacaggagcc tcgctatggg
     2521 aagtcattcc tgatccaaaa agaactgaga gagaggaagt tacagctccg gcgggtggaa
     2581 gaaattggaa aagtaattgg aatggggttc gagatatgac gcccagacaa tgcgtcggga
     2641 aaggtcggtt tcaaacattc ctgagaagat gtcttcggca gcggcgatgt agtgagagaa
     2701 ctcaaatttc tccatgattc tcagcgggcg ctgatcggca tcgcggcgct cctgctcggc
     2761 agccttcatg cgtttagcca gcacgcattg gccgttgcat tgcatttcgg gcttgtctct
     2821 gttttcgcac aactccgcag cgatgtacgt ccggttggcg ccgtaccaca tcagtactcc
     2881 ggccttgagc agcaattgcc aggcaaagag aaaaacaagc gatatggaaa tgaatgagcg
     2941 cataaaaatg tgaaggcaaa ggtatctttt tatgccggac ttggacaata tgaagtcgaa
     3001 aaaaatgacg actgccagac aaaagcgacg ctcaccactt caacaactcc gcccgtacct
     3061 cctcaaaatc tgtgaatggg atgaaatgac tacctttcgg taaatattta attatcagcc
     3121 attcagaagg aatatggcgg cggctgaagt ccaggttttc gcggggcggc gccagaaagt
     3181 ccnnnnnnnn nnagcgccat gccaatgcgt caccgggatg cgcagctcct gccagcgggg
     3241 ctgaagccgg cggagttctt ctacatgggc aaatttttca tcacccgata cctgccaggc
     3301 tttggacaac atccaacggg taagccgcca acgggccagt ccggcatacc agggtaccgg
     3361 ttcgctgtcg gggtcgttca ccggggccag catcagcagc gcctgcacct gagcggggta
     3421 gtccaatgcg acttgtgcaa taacaggccc gccgaaggaa tggcccacga gcagcgtttt
     3481 agaaggccgg tagcgatcca acacggcttt cacagcctcg gcctgcaccc gaaagctttg
     3541 tccgtaattg gaatatccgt agccggggcg atctatggaa atgaggcggt attttgccgt
     3601 caataagcta tccgacaggt atttgtaaaa attattcccc gatccgggcg cgccgtgtac
     3661 aaacaggatc aagggcgtcg agtctgtttg caggccggtt tccacatagc gcaattcgcc
     3721 gccggaaaag ggaatgcggt gaatttgcac cgatatgcct ttttcggcaa agg
//
LOCUS       DAORLF010000450         3767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13282_length_3767_cov_1.913254, whole genome shotgun sequence.
ACCESSION   DAORLF010000450 DAORLF010000000
VERSION     DAORLF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13282_length_3767_cov_1.913254"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PK198_23710"
     CDS             <1..1169
                     /locus_tag="PK198_23710"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRF41830.1"
                     /translation="RVILIVLDSVGIGELPDAALYGDQGSNTLGNIARLFPDLRLPNL
                     TAMGLGLIDPDNLLPTHPHPIAAYGKARERSAGKDTTTGHWEITGAVLDKPFPVFAEG
                     FPAELIHRFEQAIGTPVIGNVAASGTAIIEALGDEHVRSRRPIVYTSADSVFQIAMHE
                     AIYPIDKQYEICRIAREMLRGEYEVGRVICRPFEGDSGQYRRTSRRKDFSVLPPDTVL
                     DAIERSGKEVYAVGKIVDIFAGKGISRYVKTEDNAAGMRATLEALEKVENGLIFTNLV
                     DFDMLFGHRRDVPGYARALMEFDAYLPELTSKLREEDLLIITADHGNDPSFSGNDHTR
                     EYIPVLHFGARIRPGYNIGERASFADIAATIAEALEVPFVTPGQSYFQNIYQAD"
     gene            1225..2097
                     /locus_tag="PK198_23715"
     CDS             1225..2097
                     /locus_tag="PK198_23715"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRF41831.1"
                     /translation="MSRILAPTELVLNPDGSLYHCNLLPENIADTVLLVGAPDRVPKI
                     SALFDRVDFTTQKREIVTHTGIKNGKRITVLSTGMGTDNIDIVLNELDAAVNVDLKTR
                     TIKPEHKQLNFIRLGTSGSLQPDIPPDSFLAGTHGLGFDGLLHYYQNTARFTHNDFIN
                     QLVEDLYWNPLLPRPYLVEADASLLGLLPETGFYRGVTATATGFYGPQGRVLRLPVTD
                     AEMNEKLSAFRHKELRITNFEMETSALYGLSALLGHRALTVNCIIANRFTLQFSKDYQ
                     KAVIEMVGRVLEVL"
     gene            2149..>2719
                     /locus_tag="PK198_23720"
     CDS             2149..>2719
                     /locus_tag="PK198_23720"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41832.1"
                     /translation="MTKNHKKPAAEATAHPHIERNPGVPFDAAWFEALRVNRSAVERR
                     AAGLSGRRTVKKEWQAAWLLKAITCMDLTTLAGDDTEGNVLRLCAKAKAPVRQDLLEA
                     LGMGGEGLQTGAVCVYHNLIPYAKAALDGSGIPIAAVSTGFPAGKITLEDKLTEIEKS
                     VAAGATEIDIVISRDLVLQGKWRELYDEVA"
     assembly_gap    2720..2729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2730..>3062
                     /locus_tag="PK198_23725"
     CDS             <2730..>3062
                     /locus_tag="PK198_23725"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41833.1"
                     /translation="CRKACGEAHLKTILATGEIPTYTLVAKASMVSMMAGSDFIKTST
                     GKESVNATLPVSLVMIRMIRDYHERTGFKVGFKPAGGIQKAKQALDWLILMKEELGDE
                     WLFPDLFR"
     assembly_gap    3063..3095
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3227..>3409
                     /locus_tag="PK198_23730"
     CDS             3227..>3409
                     /locus_tag="PK198_23730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41834.1"
                     /translation="MKIKEIFDSMAYGPAPESAAEANAFLDARNRRFGLFINGEWREP
                     SNGAYFESTNPANRQLL"
     assembly_gap    3410..3472
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3473..>3767
                     /locus_tag="PK198_23735"
     CDS             <3473..>3767
                     /locus_tag="PK198_23735"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF41835.1"
                     /translation="QPAWAALSGHQRGRYLYAIARQIQKHSRLFAVLESMDNGKPIRE
                     TRDIDIPLVARHFYHHAGWAQLMRTELRDYQPIGVIGQIIPWNFPLLMLAWKIA"
BASE COUNT          868 a         1128 c          964 g          701 t
ORIGIN      
        1 aaagagtcat cctcatcgtt ttagacagcg taggcatcgg cgaactaccc gacgcggcgc
       61 tctacggaga tcagggcagc aatacgctgg gcaacatcgc ccggcttttt cccgacctgc
      121 gcctgcccaa cctcacggct atgggcctcg gcctcattga ccccgacaac ctcctgccta
      181 cccaccccca ccctattgcc gcgtacggca aggcccgcga acgctcggcc ggcaaagaca
      241 ccaccactgg ccattgggaa ataaccggcg ccgtactcga caagcccttt cccgtgttcg
      301 ccgagggctt tccggcggaa ctgatacacc gtttcgagca ggccatcggc acgcccgtca
      361 tcgggaatgt ggcggcttcc ggcacggcca tcattgaggc attgggcgat gaacacgttc
      421 gcagccgccg gcccattgtt tacacctccg ccgacagcgt gtttcagatt gccatgcacg
      481 aagccatcta tcccattgat aaacaatacg aaatatgccg catagcccgt gaaatgctga
      541 ggggcgagta cgaggtgggg cgcgtcatct gccgtccgtt tgagggcgat tcggggcaat
      601 accggcgcac ttcccggcgc aaagacttct cggtgctgcc acccgatacg gttttggatg
      661 ccatcgaacg cagcggcaaa gaggtgtatg cagtgggcaa aattgtggac atttttgccg
      721 gcaagggcat cagcaggtac gtcaaaacgg aggacaatgc cgccggaatg cgcgccacgc
      781 tggaggcttt ggaaaaggta gaaaacggac tgattttcac caatctggtg gatttcgaca
      841 tgctcttcgg gcatcgccgc gacgtgcccg gctatgcccg cgcattgatg gaattcgatg
      901 cttacctgcc cgaactgacc tccaagctgc gcgaggaaga cctgctcatc atcaccgccg
      961 accatggcaa cgaccccagt ttcagcggca acgaccacac ccgcgagtac atcccggtcc
     1021 tccacttcgg cgcccgcatc aggcccggat acaacatcgg tgaacgcgcc tcttttgccg
     1081 acattgccgc caccatagcc gaggctttgg aagtgccgtt tgtaacgccc ggacagagtt
     1141 attttcaaaa tatttatcag gcagattgag tgaagggggg actggcagtc cccccttcac
     1201 tcaaatgcaa aatactaacc aaccatgagc cgaattttag cgcctaccga attggtactc
     1261 aaccccgacg gcagtttgta ccactgcaac ctgctgcccg aaaacattgc cgataccgtg
     1321 ctccttgtgg gcgctcccga ccgcgtgccc aagatttcag ccttgtttga ccgcgttgac
     1381 tttacgaccc aaaaacgcga gatcgtgacg cataccggca tcaaaaacgg gaagcgcatc
     1441 accgtacttt ccaccggcat gggtaccgac aatattgaca ttgtactcaa tgaattggac
     1501 gccgccgtga acgtggattt gaaaaccaga accatcaaac cggaacacaa acaactgaat
     1561 ttcattcgct tgggcacctc cggctccttg cagcccgaca ttccgcccga cagctttctg
     1621 gcgggcacgc atgggctggg cttcgacggg ctgctgcatt attaccaaaa taccgcccgt
     1681 tttacgcaca atgatttcat caaccaactc gtggaagacc tgtactggaa cccgcttttg
     1741 ccgcgcccgt atttggtgga ggccgacgcg agtttgctcg gtttgctgcc cgaaaccggt
     1801 ttctaccggg gcgtcacggc gacggccaca gggttttacg gtccgcaggg gcgggtactg
     1861 cgattaccgg taacggatgc ggagatgaat gaaaagctct cggcattcag gcacaaggaa
     1921 ctgagaatca ccaattttga aatggaaacc tcggcactgt acggtttgtc ggccttgctg
     1981 gggcatcggg cgctcacggt caattgcatc attgccaacc ggttcacctt gcaattcagt
     2041 aaagactatc aaaaggccgt catcgagatg gtgggccggg tgctggaggt attgtgaaga
     2101 aaaaagtagt acttttgccc aaaacgcaag ttaaataaca ctgtcctaat gacaaaaaac
     2161 cataaaaaac cagccgccga ggccactgcg cacccacaca ttgagcgcaa tcccggcgta
     2221 cccttcgacg ccgcctggtt cgaggcgctc cgcgtaaaca ggagcgcagt agagcgacgt
     2281 gccgccggcc tctccggccg ccgcaccgtg aaaaaagaat ggcaggccgc ctggctcctc
     2341 aaagccatca cctgcatgga cctcaccacg ctggccggcg acgataccga aggcaatgtg
     2401 ctgcggctgt gcgccaaggc caaagcgccc gtgcgccaag acctgctcga agccctcggc
     2461 atgggcggcg aaggcctcca aaccggcgct gtgtgcgtgt atcacaacct cattccgtat
     2521 gccaaagcgg ccctcgacgg cagcggcatt cccatagcgg ccgtttccac gggattcccg
     2581 gcgggcaaaa tcaccctcga agacaagctc accgaaatcg aaaaatcggt agctgccggc
     2641 gccaccgaga tagacatcgt catcagccgc gatctggttt tgcagggcaa atggagggaa
     2701 ctctacgacg aggtggccgn nnnnnnnnnc ctgtcgaaaa gcctgcggcg aagcccacct
     2761 gaaaaccatc ctcgctaccg gcgaaattcc tacctacacc ctcgtggcca aagcaagcat
     2821 ggtgagcatg atggccggtt cggattttat caaaaccagc accggtaaag agagcgtcaa
     2881 tgccacgctg cccgtcagtt tggtgatgat tcgcatgatc cgcgactacc acgagcgcac
     2941 gggttttaag gtgggattca aacccgccgg cggcattcaa aaagccaaac aggccttgga
     3001 ctggctcatc ctgatgaaag aagaactcgg cgacgaatgg ctgttccccg acctgttccg
     3061 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattgg aacaccacgt caccgggcgt
     3121 tactccgccg cattccgtca cccaatggct tagtaaatcc aagcttgcgg cttgcagcta
     3181 acggtttgca gcctgacact aagagcttga agcaaaaaag aaacctatga aaatcaaaga
     3241 aatattcgac agcatggctt acggacccgc ccccgaaagt gccgccgagg ccaacgcttt
     3301 tctggatgcc cgcaacaggc gtttcggatt gttcatcaac ggcgaatggc gcgaaccttc
     3361 caacggggct tattttgaaa gtaccaatcc ggccaaccgg caactcctgn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacaaccc
     3481 gcttgggcag ccctcagcgg acaccaacgc ggcaggtacc tctacgccat tgcccggcaa
     3541 atacaaaagc actcgcgcct ctttgccgtg ctggaatcta tggacaacgg caagcccatt
     3601 cgcgaaaccc gcgacattga cattccattg gtagccaggc atttctacca ccatgccggc
     3661 tgggcgcaac tgatgcgcac cgaactgcgc gactaccagc ccatcggcgt catcgggcag
     3721 atcattccct ggaacttccc gctgcttatg ctggcctgga aaatagc
//
LOCUS       DAORLF010000451         3756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13325_length_3756_cov_2.031613, whole genome shotgun sequence.
ACCESSION   DAORLF010000451 DAORLF010000000
VERSION     DAORLF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13325_length_3756_cov_2.031613"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..>514
                     /locus_tag="PK198_23740"
     CDS             170..>514
                     /locus_tag="PK198_23740"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF41836.1"
                     /translation="MMKKTLLIAAVLAGVQWLSAQSNFSGGFRAGLTFATLSGPVEST
                     DSGAELEKMTLSSGFHVGATFNYKLTDNFGVRGELLYSQKGTNYDYDGESFWVFEPLI
                     GTPVYSSGTRRVS"
     assembly_gap    515..587
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <588..>1006
                     /locus_tag="PK198_23745"
     CDS             <588..>1006
                     /locus_tag="PK198_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41837.1"
                     /translation="GGVNPGILIGSRGFGELTYSGRTVLGTTIAPFTMNLEHQYSKDA
                     FRGTVGTNVENRVIEGRPVQIPLTAGAYYEGADSSEKLYKTFDLGLIGGLSFYLNKSL
                     FLGGRVHYGLSDVTNTQQDYARRAIDNNRSLILREDN"
     assembly_gap    1007..1044
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1066..1854
                     /locus_tag="PK198_23750"
     CDS             1066..1854
                     /locus_tag="PK198_23750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF41838.1"
                     /translation="MPVLQLSDTFLAYETEGQGEPVIFLNGIMMSIASWAQQRRFFSD
                     RYQCIFHDFRDQLLSGKASKSYNMDVHVQDLKQLLDHLGVQQCHVVGTSYGGEVGMLF
                     ALAHPECVKTLSVIASVPWPDALLRLQVGLWRQAAFFSGQWLYETLAAFSFSAAFLGT
                     QPDFIRSGIERVSSLPPDYFTGFARLCDAFLTLDIRVQLAEIQCPTLIVSPGADILKV
                     PYYSRYMAERIPGAQLWEIPAAGHAVVLEQPEEINRGLAEFMTC"
     gene            2071..2700
                     /locus_tag="PK198_23755"
     CDS             2071..2700
                     /locus_tag="PK198_23755"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF41839.1"
                     /translation="MGITERKQREKEEMRERILNAAIDLFLREGYDKTSIRQIADAIE
                     YSPATIYLYFKDKDELFFAIHHRGFEKLFAAMSEAAAEVNPLERLKVIGRVYLKFAIE
                     NPEFYDLMFVMRAPMAALKHRTNCGEADCDWQHGRRSYELLVNTIQECINQYLVRIPN
                     AEEGAILAWSVVHGMATLLIRERFAMIELTDPEQKMEAVLDHLIRVMRI"
     gene            2859..>3756
                     /locus_tag="PK198_23760"
     CDS             2859..>3756
                     /locus_tag="PK198_23760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF41840.1"
                     /translation="MKKRIHTVWLAVLAMLLPMWVQAQSQVLENYIQQGLAANLALRN
                     RELAVQQSEEQIRQAKGMMLPSVSFNATYTRALGGRSISLPIGDLMNPVYSTLNQLTQ
                     SNQFPQIENVKEQFLPDNFHDTRFRVVQPLYNTDLRYNKLLREQSAVIPQAAQAVHAH
                     QLRYDIEQAYYRHLQTLEALRIYRASLGVVQELHRVTQRLVDNHKVTLDAVYNTEYEL
                     SKIETEIVRAEKNVRLSANYFNFLLQRDLDAEIIADTALLQSGDLAPQPLEFYLQNAL
                     AQRQEPAQLAAAMQVNSTLLTMQ"
BASE COUNT          884 a          881 c          969 g          911 t
ORIGIN      
        1 tttttctttg taaaaaaatg aaataagccg gaaatgcctg ctgcgagcag agggccaatg
       61 cctatcttcg cggcgcattg agggccggct ttggcgcgta ccttcaataa ccacactttg
      121 cggcaaacca aaagcctgtt tcggcagtct tgttataaaa acaatcttga tgatgaaaaa
      181 aacactcctt attgcggccg tattggctgg tgtacaatgg ctgtctgcac aaagcaattt
      241 cagcggcggt ttccgggccg gcctcacttt tgccacgctt tccggccctg tcgaaagtac
      301 cgatagcggc gctgagcttg aaaaaatgac gctgtcctcc ggctttcacg tcggggctac
      361 gttcaattat aaattgaccg ataattttgg cgtgcggggc gaactgcttt attcccaaaa
      421 aggtaccaac tacgactacg acggtgagtc tttctgggtt ttcgagccgc tcatcggcac
      481 gcctgtttac agttcaggca ctcgccgggt gagtnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg cggagtcaac
      601 cccggtattc tcatcggttc gcgtgggttc ggagagttga cttacagtgg ccgcaccgtt
      661 ttgggtacga cgattgcccc attcacgatg aacctggaac atcaatacag caaagatgcc
      721 tttcggggaa ccgtgggaac caatgtggaa aaccgcgtca tcgaagggcg gccggtacag
      781 attccgctga cagccggcgc atattatgaa ggcgctgaca gcagcgaaaa actctacaaa
      841 accttcgatc tcggtctcat cggcggcttg tcgttttatc tgaataaatc tcttttcctc
      901 ggcgggcgcg ttcactatgg tctttccgat gtgaccaata cacagcagga ttatgcacgt
      961 cgcgccattg acaacaaccg cagcctcatc ctccgcgagg ataatgnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnttcagc ctctgataat tatacatgcc tgtactccaa
     1081 ctttccgaca ccttcctcgc gtatgaaacc gaggggcagg gcgaacccgt tatttttctc
     1141 aacggcatca tgatgagtat tgcctcctgg gcgcagcagc gcaggttttt tagtgaccga
     1201 taccagtgca tttttcatga tttcagggat caattgctca gcgggaaagc ctcgaaatcg
     1261 tacaatatgg atgtgcatgt gcaggatttg aaacaactct tagatcacct gggggttcag
     1321 caatgccatg tcgtcggcac ctcttatggc ggcgaggtag gcatgctgtt tgccttggcg
     1381 catccggagt gcgtgaaaac tttgagcgtg atagcctccg taccctggcc cgatgcgctg
     1441 ctgcgccttc aggtagggct gtggcgacag gcggcgtttt tttccggtca atggttgtac
     1501 gaaaccctgg ctgcgttctc tttttccgcg gcatttctgg ggacgcaacc cgattttatt
     1561 cgcagcggca tagaacgcgt gtcatctttg ccaccagatt attttacggg ttttgcccgg
     1621 ctttgcgacg cgtttcttac tttagatatt cgagtgcaac ttgcagaaat tcaatgccct
     1681 acgcttattg tatcccccgg agccgatatt ttgaaagtgc catattacag tcggtacatg
     1741 gccgagcgca ttcccggcgc ccaactctgg gagatacccg ctgcgggcca tgcggtcgtg
     1801 ttggagcaac cggaggagat caatcgggga ctggcggagt ttatgacgtg ctgatctcga
     1861 ttttccgatt cccttcgtcc atcggaacat gctctgcgtc gtcgggataa gttgtgacaa
     1921 tgtgctcatt tacagatttt tagtagaatg agtgctatgg ctccaatcct tgaatatctt
     1981 ttttttgaaa aaaaaaataa ccctaagact tgatttcact aaacactgtt cagtatattt
     2041 gcatcgcaat tcaaaattat tcaattttag atgggtatca cagagcgcaa gcaacgggag
     2101 aaagaagaaa tgcgggagcg catcctgaat gctgccatag atttgttttt gagggagggc
     2161 tacgacaaaa ccagtatccg gcaaatagcc gacgccattg agtacagtcc ggctactatc
     2221 tatctgtatt tcaaggataa agatgaactg ttttttgcca ttcaccatcg gggttttgaa
     2281 aaattatttg ccgccatgtc ggaagcggcg gcagaggtga accccttgga acgactcaaa
     2341 gtcatcgggc gggtgtatct gaaattcgcc atcgaaaacc ccgagtttta cgacctgatg
     2401 tttgtgatgc gcgctccaat ggcggctttg aagcatcgta ccaattgtgg ggaggccgat
     2461 tgcgactggc agcatggacg cagaagctac gagttgttag tcaatacaat acaggagtgc
     2521 atcaaccaat atttggtgcg cattcccaat gccgaagaag gcgccattct ggcctggtcg
     2581 gtggtgcatg gtatggctac cttgctcatc cgggagcgat ttgctatgat agaactcact
     2641 gatcctgagc agaaaatgga agcagtgctg gatcatttga ttcgggtgat gaggatttga
     2701 gagggggaga aagagagact tggagagggg gagactttga gaaagggaga ctttgaaggg
     2761 ggggagagac ttggagaaag agtgactggg gctttgatcc cttatttttt gaatttttac
     2821 taaacaatgt ttacttaata tacgattaac attcaagcat gaagaagcga attcatacag
     2881 tgtggcttgc cgtgttggcg atgctgttgc ctatgtgggt gcaagcccag agccaggtgt
     2941 tggaaaacta catacaacaa gggttggcgg ctaatctggc cttgcgcaac cgggagttgg
     3001 cggtgcagca aagcgaagag caaatccggc aggccaaagg aatgatgctg ccgtcggtga
     3061 gttttaatgc cacttatacg cgggcactcg gcgggcgcag catctcattg cccatcggcg
     3121 atctgatgaa tccggtttat agcacgctca atcaactgac gcaaagcaac cagtttccac
     3181 aaattgaaaa cgtgaaggag cagttcctgc ccgacaattt ccatgacacc cgctttcggg
     3241 tggtgcagcc tttgtacaat accgacctgc gctacaacaa gctgctccgc gaacagagcg
     3301 ccgttatccc gcaggctgcg caggccgttc atgcccatca attgcgctac gatatagagc
     3361 aggcgtacta ccggcacttg cagactttgg aggcgctccg catttatcgc gcatcgctgg
     3421 gggtggtgca ggaattgcac cgggtgacgc agcgcttagt cgacaaccac aaagtaaccc
     3481 tcgacgcggt gtataatact gagtacgaac tgagcaaaat agaaacggaa attgtgcgcg
     3541 cagagaaaaa tgtgcgcctg agcgccaatt atttcaactt tctgttgcag agggatttag
     3601 atgcggaaat cattgccgat acagccctgt tgcaatcggg agacttggcg ccacaacccc
     3661 tggagtttta tctccaaaat gccctggccc aacggcagga acccgctcaa ttggccgccg
     3721 ccatgcaggt gaattctact ctgctgacca tgcaag
//
LOCUS       DAORLF010000452         3751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13349_length_3751_cov_1.956439, whole genome shotgun sequence.
ACCESSION   DAORLF010000452 DAORLF010000000
VERSION     DAORLF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13349_length_3751_cov_1.956439"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>782)
                     /locus_tag="PK198_23765"
     CDS             complement(134..>782)
                     /locus_tag="PK198_23765"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF41841.1"
                     /translation="RHQMLDEFIAGIENTGAVSGVAIEKEVWVGFPADEIIKHSHEQD
                     LIIMGTTGEGGILNKVFGSVSSAVAQRADCPVILVPTEADFKPIHHILYASHYEAADQ
                     RMVAQLLEFNRAFKAHIHFVHVRDAGSDRFTQEKEGIFSTLFAGGEPSFAFEIAELEA
                     DSVAEALNEYAEQHHTELVVLVTRRRNLWEQLFHKSQTRNMARITRKPVMVLHLN"
     assembly_gap    783..837
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1116..1556
                     /gene="rnpB"
                     /locus_tag="PK198_23770"
     ncRNA           1116..1556
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK198_23770"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     assembly_gap    1617..1648
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1649..1940)
                     /locus_tag="PK198_23775"
     CDS             complement(<1649..1940)
                     /locus_tag="PK198_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41842.1"
                     /translation="MKFWKQLMLVALLVAGCAVTSIAQQAAAAEAPAKQKPSATYQVG
                     SAKITVWENKRPDGSTWKNFVVEKVYRKNGEWHTAKSFDEGELLELKAAIDKA"
     gene            complement(1982..2926)
                     /locus_tag="PK198_23780"
     CDS             complement(1982..2926)
                     /locus_tag="PK198_23780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF41843.1"
                     /translation="MPLPDFQMLANRLHKMHRHIGKWARRQGITCYRIYDADIPEFPL
                     AIDWYEGSLHVAEYERDHPLDEEEYQIWRAGCRQIMAETLEVAPEQVWFKSRSRQKGA
                     AQYEKVATRSHEVIVHEDGLQFKVNLSDYLDTGLFLDHRQTRKMVRQLSKGKSVLNLF
                     AYTGSFTVYAAAGGAAATTTLDMSATYLQWAEENLKINELNGSQHRFIQTDVTAWLLN
                     EPIEKFDLIVLDPPTFSNSKRMRHDFDIQRDHTYLINRCLHRLHPGGTLFFSTNFRKF
                     KLDTERIWGGARITDISNKTVPEDFRNKKIHYCFQIDV"
     gene            complement(3082..3555)
                     /locus_tag="PK198_23785"
     CDS             complement(3082..3555)
                     /locus_tag="PK198_23785"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRF41844.1"
                     /translation="MKILMVCLGNICRSPLAEGIMQAKAQANNLHWKVDSAGTGAWHA
                     GERPDSRSIRTGRNHGFDIARQRARQVRKDDFDHFDLILAMDSSNYEDLLGLAQTDEQ
                     RSKIAMIMNFLHPGENRDVPDPYYHNQLFEEVYHMLDAACDAVITQFTTHTPPKT"
BASE COUNT          777 a          849 c         1120 g          918 t
ORIGIN      
        1 taaaatgaat aaggtagagc tggtgattgc ttcgccgggg ggcgatctgg tatttcataa
       61 tgcaccggat actcctgcaa aatcagacgc gcctgccaca aaagaggcgc cggccgaaaa
      121 gaagaagcag taatcaattg agatgcagca ccatcaccgg tttgcgggtg atgcgcgcca
      181 tgttgcgggt ctgactcttg tggaagagtt gttcccatag attgcgccgt cgggtgacta
      241 aaactaccaa ttcggtatgg tgctgctctg cgtattcgtt gagggcctcg gcaacggaat
      301 cggcttccag ttcggctatt tcgaaggcaa aagacggctc gcccccggca aagagcgtgc
      361 tgaaaatccc ttccttttcc tgagtaaagc gatcgctgcc ggcatctcgg acatgcacaa
      421 aatggatgtg tgctttaaat gcgcgattaa attcgagcaa ttgcgctacc attcgctggt
      481 cggcagcttc gtaatgactg gcgtatagaa tatgatgaat gggtttgaaa tctgcctcag
      541 tggggaccag tatcaccggg caatcggctc tttgtgctac tgcggaagat acgctgccga
      601 aaactttgtt gagaatgcct ccttcgccag tagtacccat gatgatgagg tcttgctcgt
      661 gagagtgttt gatgatttca tcggcgggaa aacccaccca gacctctttt tcaatagcta
      721 cccctgaaac cgccccggtg ttttcgatgc ctgcgataaa ttcgtcaagc atttgatggc
      781 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga
      841 ataaaattgg gatattcgga gtccacctgc ggcataaaca catacacgac tttgatggac
      901 ggccgcccct ggcgggctgc aaactgaagc gcatatcgga ctgcattggc ggcggtgtcg
      961 gaaaaatcaa caggaaccag aatattgttc atcatggcat ttgtttttct gaaacgaaga
     1021 tgctcacatt tcatttagcg cgcgttgata ttcatcaatg tgggcggtga tttttgaaac
     1081 tttggcctca aacatgtagt tttgcccttc gaagtgccgc caaacgctct atcgcggctg
     1141 cgcatggcgc ggctgaggaa agtccggaca acgcagcgcg ccgcaccacc taacgggtgg
     1201 gattcgcagt gggcaaccac gcgaagccgg aaagtgccac agaaaagata ccgcctcgcc
     1261 tcggcggggt aagggtgaaa tcgcggtgta agagaccacg ccgcccggca gcaatgcggg
     1321 cgagggtaaa ccctgcgggt tgaaatgcca tgtaaacctc aattttgcca gaggcgcttc
     1381 ggcgaaccct gagcgaagac gggttgctcg cccgttcacc gcgctttggc gcggaggttg
     1441 aggagggtag gcagagcaga ctcctgccgc gaggctggag ccagataaat gatagaggct
     1501 gtgcgcgttg tgaggcgcgg gcagtacaaa atccggctta cagcttggcg gcgcttcgtt
     1561 ttttttatca taccccctcg cgctgcgcag agcgttaatc ttcttctttc ccttctnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gctttgtcta tggctgcttt cagctccagc
     1681 agttcgcctt cgtcaaaact tttggcggtg tgccattcac cgtttttgcg atatactttt
     1741 tccaccacga agtttttcca ggtggagccg tcagggcgtt tgttttccca cacagtgatt
     1801 ttggcggagc cgacctgata ggtagcagag ggtttttgtt tggcgggcgc ttcggcggcg
     1861 gcggcctgct gtgcaatgga ggtgactgcg catcctgcaa cgaggagggc aacgagcatc
     1921 aattgtttcc agaatttcat ggcgagtcat tttttagttt gaatgccagc aagatatgac
     1981 ttcacacgtc aatttggaaa cagtagtgga tttttttatt gcgaaagtct tccggcacgg
     2041 ttttgttgct gatgtcggtg atgcgggcgc cgccccagat tctttcggta tcgagtttga
     2101 attttcggaa attggtagag aaaaagagcg tgccaccggg atggagccgg tgcaggcaac
     2161 ggttgatgag ataggtgtgg tctctctgga tgtcgaaatc gtggcgcatc cgtttggagt
     2221 tggagaaagt aggcggatcg aggacgatga gatcaaattt ttcgatgggt tcgttgagca
     2281 gccaggcggt gacgtcggtt tggataaacc ggtgttgcga gccatttaat tcattgattt
     2341 tcaaattttc ttcggcccat tgcaggtagg tggccgacat gtcgagggta gtggtggctg
     2401 cggcgccgcc ggctgcggca tacacagtga aggagccggt gtaggcaaag aggttgagta
     2461 cgcttttgcc ctttgacagt tggcgcacca ttttgcgggt ttgccggtga tctaggaaaa
     2521 ggccggtatc gagataatcg ctgaggttga ccttgaattg caggccgtct tcatgcacga
     2581 tgacttcgtg gctgcgcgtt gccacttttt cgtattgagc ggcgcctttc tggcggctgc
     2641 gggatttgaa ccagacctgt tcgggcgcta cttcgagggt ttcggccatg atttggcggc
     2701 agccggcgcg ccaaatctgg tattcctctt catctaaggg gtgatcgcgc tcatactcgg
     2761 cgacgtgcag gctaccctcg taccagtcaa tggcgagggg gaactcagga atatctgcat
     2821 cgtaaatccg gtagcaggtg atgccttgcc ggcgcgccca tttgccgatg tggcggtgca
     2881 ttttgtggag gcggttggcc agcatttgga agtcaggtaa cggcatggaa cgggtggatt
     2941 tcggatttct aatttcggat ttctaatttc gggtactcgt ccgttgcgtg agggggccgc
     3001 cggaaggacg acgataggag gacgccccag gcacccgacg gcgcatgggt atgtgcgaga
     3061 gcgccggcac gcccagaaat gtcaggtttt agggggtgta tgggttgtga attgtgtaat
     3121 gacggcatcg caggcggcgt cgagcatgtg atagacttct tcgaagagtt ggttgtggta
     3181 gtatggatcg ggcacgtcgc ggttttcgcc ggggtggagg aagttcataa tcatggcgat
     3241 tttactgcgt tgctcgtcgg tttgggccaa gccgaggagg tcttcgtagt tgctgctgtc
     3301 catagcgagg atgaggtcga agtggtcgaa atcgtctttg cgcacttggc gggcgcgttg
     3361 gcgggcgatg tcaaaaccgt gattgcggcc ggtgcggatg gagcggctgt cggggcgttc
     3421 gccggcgtgc catgctccgg tgcctgcgga gtccaccttc caatggaggt tgttggcctg
     3481 ggcttttgcc tgcataatac cctcagccaa gggcgagcga caaatatttc cgaggcaaac
     3541 cattagaatt ttcatggtgt caatggattt atattctaaa ccaaacgata tttgtccctt
     3601 gaaaatcaac gggcttcaca aattgcggta ataaacactg ctttgggaag taaagttgaa
     3661 gcccggcgcg accatacgag ccgggttcac caaacgcaaa attgtatgag agttgtaatt
     3721 gccggcgcag gggatgtggg gtttcacctt g
//
LOCUS       DAORLF010000453         3721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13494_length_3721_cov_2.154664, whole genome shotgun sequence.
ACCESSION   DAORLF010000453 DAORLF010000000
VERSION     DAORLF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13494_length_3721_cov_2.154664"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>258
                     /locus_tag="PK198_23790"
     CDS             30..>258
                     /locus_tag="PK198_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41845.1"
                     /translation="MNKHAYSAVEVHDLRLVFEEVTGEDLNWFFNQWFLKAGHPTLNI
                     TYGFDAEAGKATVTVEQTQDPELMPAIFELPT"
     assembly_gap    259..268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <269..1241
                     /locus_tag="PK198_23795"
     CDS             <269..1241
                     /locus_tag="PK198_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41846.1"
                     /translation="LYFDNGTKRREKIHITERGQTFEFEVPSRPALMVFDPQKVLLCI
                     REEEKSEQEYVFQYFNAPSLSDRMDALQYIVENESEEMPRVFNAGLNDKFWLIRAVST
                     AYADFEEEPIIKTIRGMAKSDPHSVVRREAMNNLQQWEDEEALLVARYILEKDSVNSV
                     LASALSVLVAQDSDAAMEYARKWENTQSEDILSIIGELYSSKKDPAHLPFFERQLAKT
                     DGYAALSVFGAYQSLVAELDLDKAAPALEKIETIAKGEKESLWRRFASFKFLVDMSNA
                     YAQKVDSPAHTAKAAELRKKAEALRDAETDADMKGIYEQVLQGAGGE"
     gene            complement(1373..>3721)
                     /locus_tag="PK198_23800"
     CDS             complement(1373..>3721)
                     /locus_tag="PK198_23800"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HRF41847.1"
                     /translation="VTTTELVSGTNLPIFNAFNRPERIPANFGQALRLDGYSTYIRNT
                     GFPIQGYAGRLTVEAWYATEAFTFEPGAIIQSQSGNNGFKLQVNSIGAVAFSFSFNGQ
                     LRNLVTNSRLAPYRWHHIVAVVDGIIGNVTIYVDNESWLSSNFSPNSTINSADATLWV
                     GKSALNQVFAGFSLNTLNGALDDLKVYNTALTVDEIAAHYAQATQLEAPLSIDPDVRH
                     AGDYLRPRYHPMPNTSWTNEPYGLTYYDGKYHLFFQKNPNGPYLYFMHWGHLSSPDLI
                     TWTEEPIALAPFAGFDSFGVWSGTTAFDPNGEPTIFYTGVNGASAGIGVARPIDEDLR
                     RWQRVPGNPVIPSAPGGFPHLDFRDPYIFQRNGLYYMVVGSGLANNGGGILFTYTSTD
                     LNIWTMAQPIFSSTDFIHHGTFWEMPSFFPMNENDYAFVVTPLFQGQPADVIYWVGSF
                     DGQRFTPYDNTARNFELISQNLLAPAIGRDQNGQWTSIGIIPDDRAVNLQIEAGWRHI
                     FSIPRQLRLLEDHRTLAAIPHLCRLRLDSVVITNRSILANTPGNLSEFRSNQAELDIE
                     IRVPANGQTYVDVFKSENNQERTSIILDRLNGQIGLDRSVSSPYSTVENIRYHSYDFN
                     PSGIVKLRVFLDHSVVEVFVDDKMSFSARAYPGPASNRVDVRGPNSAAEILTFKGYQL
                     GDKENLYPDLVCPADVFSRVAELPSFNKLHVFPNPVQNQLIIQNEVLYPAEVVITDLY
                     GRTHGDVRMEGNVLNVRHLSAGVYVLTMHQRNDVFIARFIKQ"
BASE COUNT          953 a          919 c         1002 g          837 t
ORIGIN      
        1 aagcattttt tgaggcgctg aaattatatt tgaacaaaca tgcctattcc gcagtggagg
       61 tgcatgatct gcgcttggtt tttgaagaag ttaccggcga agacctcaac tggttcttca
      121 atcagtggtt cctcaaagcc ggccacccca cgctcaacat cacctacgga tttgatgctg
      181 aagcaggcaa agctaccgtg accgtagaac aaacgcaaga ccccgaactg atgcccgcca
      241 tttttgaact ccccaccgnn nnnnnnnncc tctacttcga caacggtacc aagcgccgcg
      301 aaaaaatcca catcaccgag cggggccaaa cctttgaatt tgaagtgccc tcgcgcccgg
      361 ccctcatggt gttcgaccct caaaaagtgc tgctttgtat tcgcgaagag gaaaaatcgg
      421 agcaggagta tgtgttccag tatttcaatg caccctcttt gtcggatcgg atggatgcgc
      481 tgcaatatat tgtggagaat gagagcgaag aaatgccgcg cgtcttcaat gccggattga
      541 acgataaatt ctggctcatc cgtgccgttt ccaccgctta tgccgatttt gaggaagagc
      601 cgatcatcaa aaccattcgc ggcatggcca aatccgaccc gcactccgta gtgcgtcgcg
      661 aggccatgaa caacctccag caatgggaag acgaagaagc gctgttggtg gcgcgttata
      721 ttctggaaaa agactccgtg aacagcgttc tggcatcagc cctctctgta ctggtagccc
      781 aggattccga cgccgccatg gaatacgccc gcaagtggga aaacactcaa agcgaagaca
      841 ttctgtccat catcggcgag ttgtacagtt ccaaaaaaga cccggcgcat ctgccgttct
      901 tcgagcgcca attggccaaa accgacggat atgcagccct ttccgttttc ggggcatacc
      961 agtctttggt agcggaattg gatttggaca aagcggctcc tgctcttgaa aaaattgaaa
     1021 ccattgcaaa aggagaaaaa gaatctctgt ggcgccgttt tgcaagcttc aaatttttgg
     1081 tggacatgag caatgcttat gcccaaaagg ttgattctcc ggcacacacc gccaaagctg
     1141 ccgaactgcg caaaaaagca gaagccctgc gcgacgctga aaccgacgcc gacatgaaag
     1201 gcatttatga gcaggtattg cagggcgcgg gaggcgagta agggattgtg ctttcaacgg
     1261 cgtggcaaac atgcccggcg acaaccaacg cttcgatcac acgaaggcga acgcccggtg
     1321 gccaatgcgg cggtgaggcg gcaggctgta aaatgccccg ccccggatgc cgctactgtt
     1381 ttataaaacg agctataaaa acgtcattcc tttgatgcat cgtcagcaca tatacgcctg
     1441 cggacagatg cctcacgttg aggacattgc cctccatgcg tacatcgcca tgcgtgcggc
     1501 catataggtc ggtgatgacc acttctgctg ggtacagcac ttcgttttgg atgatgagtt
     1561 gattctgaac aggattggga aaaacatgca atttgttgaa ggacgggagc tctgccaccc
     1621 tgctgaaaac gtcagccggg cagaccaagt ccgggtaaag attttctttg tcgcctaatt
     1681 ggtagccttt aaaagtaagg atttcggcag cgctgttcgg cccgcggaca tcaaccctgt
     1741 tggaggccgg gccggggtat gctctggcag agaaagacat tttgtcgtcc acaaaaacct
     1801 cgacaacgga atggtcaaga aatacccgga gtttgacgat tcccgatgga ttgaaatcat
     1861 agctgtggta gcgaatgttt tcaaccgttg aatacgggga agaaacactt cggtcaagcc
     1921 cgatttgccc attcaaacgg tctaaaatga tgctggtgcg ctcctgattg ttttcgcttt
     1981 taaagacatc cacataggtt tggccgttgg cgggcacgcg tatttctata tccaattcag
     2041 cctgattgct cctaaactca ctcagattgc ccggggtatt ggccaggata ctccggttgg
     2101 ttatgacaac ggagtccaag cgaagccggc acagatgagg aatagcagcc agggtacgat
     2161 gatcctccaa taagcgcaat tgcctgggaa tgctgaaaat atgacgccag cctgcttcaa
     2221 tttgtaaatt cacggccctg tcgtcgggaa taataccgat gctggtccat tggccgtttt
     2281 ggtcacgccc aatggccggg gccagtaagt tttgagaaat cagttcaaag ttgcgcgcgg
     2341 tattgtcata aggcgtaaat cgctggccgt caaaactgcc tacccagtaa atgacatctg
     2401 ccggttgccc ctggaacagt ggcgtcacta caaacgcata atcgttttca ttcatcggaa
     2461 aaaaagaggg catctcccaa aaagtgccgt gatgaatgaa gtcggtgctg ctgaatatgg
     2521 gctgcgccat ggtccagatg ttcaaatcag tagaagtgta ggtaaataaa ataccgcctc
     2581 cgttattggc caggccagac cccaccacca tgtagtacag tccattccgc tgaaaaatat
     2641 aggggtcccg aaagtcgaga tgaggaaatc cgccgggagc ggagggaatg accggattgc
     2701 cgggcacgcg ctgccatctt ctcaaatcct cgtctatggg gcgcgccaca ccgatgccgg
     2761 cacttgcccc gttgacgccg gtgtaaaaaa tggttggttc gccgttggga tcaaaggctg
     2821 ttgtgcccga ccatacgccg aagctgtcga agcctgcaaa gggcgccaac gcaatgggtt
     2881 cttccgtcca ggtgatcaag tcggggctgg acaagtggcc ccaatgcata aagtagaggt
     2941 aaggcccgtt gggatttttt tgaaaaaaca ggtggtactt gccatcgtaa taggtcaatc
     3001 cgtatggttc gttggtccat gaagtattgg gcattgggtg ataccgcggg cgcaggtagt
     3061 cgccggcatg gcgaacatcg gggtcaatag aaagcggggc ctccaactgg gtagcttgtg
     3121 cgtaatgcgc tgcgatctca tcaacggtaa gcgcagtatt gtacactttt agatcgtcca
     3181 aagcgccatt gagcgtattg agcgaaaacc ctgcaaaaac ctgattgagg gcgctttttc
     3241 caacccatag ggtggcgtcg gctgagttga tggtcgagtt gggactgaag ttggaactga
     3301 gccagctttc gttgtcaaca taaatggtta cattaccgat tatgccgtca actactgcta
     3361 cgatatggtg ccagcggtaa ggagcaaggc ggctgttggt gaccaaattg cgcaattggc
     3421 cgttgaagga aaaagaaaaa gctaccgcac cgatggagtt gacctgcaac ttgaatccgt
     3481 tgttgcccga ttggctttgg ataatggcgc ccggctcgaa agtaaaagct tcggtggcat
     3541 accaggcctc aaccgtgagc cggccggcat atccctgaat gggaaaacca gtattgcgaa
     3601 tgtaagtaga ataaccatct aagcggagcg cctggccaaa gttggcgggt attcgctccg
     3661 ggcgattaaa ggcattaaaa atagggagat tggttccgga aaccaattcg gtagtcgtca
     3721 c
//
LOCUS       DAORLF010000454         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13562_length_3710_cov_1.798632, whole genome shotgun sequence.
ACCESSION   DAORLF010000454 DAORLF010000000
VERSION     DAORLF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13562_length_3710_cov_1.798632"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>374)
                     /locus_tag="PK198_23805"
     CDS             complement(<1..>374)
                     /locus_tag="PK198_23805"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF41848.1"
                     /translation="HAMVVTGYDDRKGAFRLMNSWGQNWGDQGFIWIKYNDFGRFCKY
                     AYVMRLAAEDMPEMTENQSEEPAVRPLQRLSGKLEFRSLEGWQPQSGAPVFETQAVQR
                     QGFVYQLRRQDWQTGQLFQLIL"
     assembly_gap    375..387
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<388..>749)
                     /locus_tag="PK198_23810"
     CDS             complement(<388..>749)
                     /locus_tag="PK198_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41849.1"
                     /translation="ALFLYNQIKTGDCTQGARISDALRLLQQKGNCLAGQFDFDVNNC
                     EQQPDERTVETARRFSISDYMTLFGQNEDPAQKTALTKRVLAAGKPVVVGMNVLRNFY
                     QLQNALYWHPELGNTAPAG"
     assembly_gap    750..774
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<775..1078)
                     /locus_tag="PK198_23815"
     CDS             complement(<775..1078)
                     /locus_tag="PK198_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41850.1"
                     /translation="MRYLLLYLLFVGTAQLAAQSEPAYAGGLLEEDERYESLPRMAPE
                     DGAKAVLPVSVDWSAYCPEVRHQGYIFSCVGWATGYAALSIQRAMLNQCTDRALITR"
     gene            complement(1099..2007)
                     /locus_tag="PK198_23820"
     CDS             complement(1099..2007)
                     /locus_tag="PK198_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41851.1"
                     /translation="MKTLLLSILCCFLFAAGTQAQPDKKTAPALHNFLNTEFMLKFRD
                     LRIECESKVLQTQASSMQLDPQLVFRLRSAHDQTATRANQLLENIKQDFLNEKKLKSI
                     AEFPEMYSDGLRFKLQDISDFYNTNFMQALADASIPQEQVDGGAILLLVVELVGLVNG
                     LTKHFSDIRREARFYNDKYLQDNLVKPYRWQYWDELAGGNSRYEQFDRPSELIRPDAS
                     RDEMLQQRLQRVNETVNQVKNSNTQQGAPADWQYQPDPIPATPPADSTGGQWSPLKTK
                     ATTTTPDPAGKKTDTKKVETPAKKDH"
     gene            complement(2332..>3343)
                     /locus_tag="PK198_23825"
     CDS             complement(2332..>3343)
                     /locus_tag="PK198_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF41852.1"
                     /translation="QKFHAAIKDKVTGYAMQLRLNGAPNQTLIWNWAQTPANSSLGWN
                     LDRRMHVASVSKLITGIAMLKLLDEKGISVDAKIINYLPDYWAKGNNIGQISFRNLLQ
                     HRSGFSTGGSSSSFNTMKTRVAAGVPGVGGYDYENMNFGLCRILISVINGNINKNANF
                     GVLNDQIWDMVTISSYRNYVQTKVFNPAGVANAGFAPAAGGALAYRFPHGNQAGWNSG
                     DLSTMSGGAGWRLSVTEVLKVMDPARRKNTILPANRTQFLLDNRLGIDQIINTPAGKL
                     YNKNGAWRDGDCANNPRQEQCVAVFLPNGYEIVVFVNSRIGAGCESLRGLVTETYLDS
                     FQ"
     assembly_gap    3344..3487
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          646 a          849 c         1190 g          843 t
ORIGIN      
        1 gcaaaatgag ttgaaacaac tgccccgtct gccagtcctg ccggcggagt tgatacacaa
       61 aaccctggcg ctgcacggcc tgcgtttcaa aaaccggtgc gcccgactgc ggctgccaac
      121 cctccaaact ccgaaattcc agcttgcccg aaaggcgttg caaggggcga acagccggct
      181 cttccgactg attttcagtc atttccggca tgtcttcggc ggctaagcgc atcacataag
      241 catatttgca aaagcgcccg aaatcgttgt atttgatcca gatgaagcct tggtcgcccc
      301 agttttggcc ccaggaattc atcagcctga aagcgccttt gcggtcgtcg tagcctgtca
      361 ctaccatagc atggnnnnnn nnnnnnnccg gcgggcgcgg tgttgcccaa ttcggggtgc
      421 cagtacaggg cgttttggag ttggtaaaaa ttgcgcagca cgttcatgcc taccaccacg
      481 ggtttgccgg cggccagcac gcgtttggtc agcgctgttt tctgtgccgg gtcttcattt
      541 tggccaaaga gcgtcatgta gtcgctgatg ctgaaacggc gggcggtttc cacggtgcgc
      601 tcgtcgggtt gctgttcgca attgttcacg tcgaagtcga actgcccggc cagacaattg
      661 cctttttgct gcaacaggcg cagcgcgtcg ctgatgcggg cgccctgggt acagtctccg
      721 gttttgatct ggttgtacaa aaacaaggcn nnnnnnnnnn nnnnnnnnnn nnnntgcggg
      781 tgatgagtgc gcggtcggtg cattggttca gcatggcgcg ctgtatgctg agggccgcat
      841 agccggtagc ccaacccacg caggaaaaaa tgtacccctg atgccgcact tccgggcagt
      901 aggccgacca gtccaccgat acgggcagca cggccttggc gccgtcttcg ggagccatgc
      961 gcggcaggct ttcgtagcgc tcgtcttctt cgagcaggcc gccggcgtag gcgggttcgc
     1021 tttgtgcggc gagttgtgct gtgccgacaa agagcagata aaggaggagg taccgcatgt
     1081 tgttgagagt tataatattc agtgatcctt cttcgccggt gtttccactt ttttggtgtc
     1141 cgtttttttg ccggcggggt cgggcgtagt ggtagtggct ttggttttca ggggcgacca
     1201 ctgccctccg gtgctgtcgg cgggcggggt ggccggtatt gggtcgggct ggtattgcca
     1261 gtcggcggga gcgccctgct gcgtgttcga gttttttacc tgattcacgg tttcgttcac
     1321 gcgttggagg cgctgctgga gcatttcatc gcgggaggca tcggggcgga tgagttcgga
     1381 tgggcggtcg aactgttcgt agcgggaatt gccgccggcg agttcgtccc agtattgcca
     1441 gcgatagggc ttcacgaggt tgtcctgcaa gtatttatcg ttgtaaaacc tggcttcgcg
     1501 gcggatgtcg ctgaagtgtt tggtgagacc gttgaccaag cccaccaatt ctaccacgag
     1561 cagcaggatg gcgccgccgt ccacttgttc ctgcgggatg gaggcatcgg cgagggcctg
     1621 catgaagttg gtgttgtaaa aatcggagat gtcctgcaat ttgaagcgca gaccgtcgga
     1681 gtacatctcc gggaattcgg cgatggactt gagttttttc tcattgagga agtcctgctt
     1741 gatgttttcg agcagttggt tggcgcgggt ggcggtttgg tcgtgggccg agcgcaggcg
     1801 gaagactaat tgcggatcga gttgcatgga ggaggcctgg gtttgcagca ccttgctttc
     1861 gcactcgatg cggaggtcgc ggaatttgag cataaactcc gtgttgagga agttgtgcag
     1921 ggccggcgcg gtttttttgt cgggctgggc ctgagtgccg gcagcaaaaa ggaagcagca
     1981 aagaatagac agcagtaagg ttttcatggc aatgatgtgt ttttttggag aatgtgtcgg
     2041 cagcgggaag aatgtaccca atatcggagc gatttttttt gtctcggtga gaaaggccgt
     2101 tgcgccaggg ataggaacgg aatggcggcg ggcagggata gtagagacat tgcatgcaat
     2161 gtctctaccc ttgcccgccg ccatgcagtg gatagcccgc cacgcccgcc cgatggctcc
     2221 ctccattgga gggtcgggga ggcaggtagt ggcgtggggc gcccaaaaat aaaaaacagc
     2281 cgacgccgaa gaacgacgcc gactgaagat tgggacgata acggttggtt attattggaa
     2341 gctatctaag taggtttccg tcactaagcc gcgcagggat tcgcagccgg cgccgatgcg
     2401 ggagtttacg aagaccacta tttcgtagcc gttgggcagg aagaccgcca cgcactgctc
     2461 ctggcgaggg ttgttggcgc agtcgccgtc gcgccatgcg ccgtttttgt tgtacaattt
     2521 gccggccggc gtgttaatga tctggtcaat gccgaggcgg ttgtcgagca ggaattgcgt
     2581 gcggttggcg ggcaggatgg tgtttttgcg gcgggcgggg tccattactt tgagcacttc
     2641 ggtgacggag agtctccagc cggcgccgcc gctcatggtg gagaggtcgc cggagttcca
     2701 gccggcctga ttgccgtgcg gaaagcggta ggccaatgcc ccgccggctg cgggagcgaa
     2761 acctgcgttg gctacgcctg cggggttgaa caccttggtt tgcacatagt tgcgatagga
     2821 actgatggtg accatatccc agatttggtc gttgaggacg ccgaagttgg cgtttttgtt
     2881 gatgttgccg ttgatgacgg agatgaggat gcggcagagg ccgaagttca tgttttcgta
     2941 gtcgtagccg cccacgccgg gaacgccggc tgccacgcgg gttttcatgg tgttgaagct
     3001 ggaggaggag ccgcctgtac tgaagccgga gcggtgctgg aggaggttgc ggaagctgat
     3061 ctggccgatg ttgttgcctt tggcccagta gtcgggcagg tagttgatga ttttggcgtc
     3121 cacggagatg cctttttcat cgagcagctt gagcatggcg atgccggtga tgagcttgct
     3181 gacgctggcg acgtgcatgc ggcggtctaa gttccagccg aggctgctgt tggcgggcgt
     3241 ttgcgcccag ttccagatca gggtttgatt gggcgcgccg ttgaggcgca gttgcatggc
     3301 gtagccggtc actttgtctt tgatggcggc gtggaatttc tgcnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnccg gtgggcaggg gcgtggtggt gatttgctgg gcatgtgcaa acgcgcccac
     3541 aaaaagggca aaggcgatgc tgagaatgga aaagattttg gtggttttca tggttgaaaa
     3601 aaggttttgg atatggtatg aaaaaaagat ttgtgcctac tctgatcgct gcttttcggc
     3661 ttccgcagtg aatgccacag tgcgtggtgt tcgagggaat gtgtcgggag
//
LOCUS       DAORLF010000455         3708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13570_length_3708_cov_1.994525, whole genome shotgun sequence.
ACCESSION   DAORLF010000455 DAORLF010000000
VERSION     DAORLF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13570_length_3708_cov_1.994525"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK198_23830"
     CDS             <1..202
                     /locus_tag="PK198_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41853.1"
                     /translation="PEREFLTRILAKVANFRECEGKCDLYALHGLQPAAISRQPFPPE
                     RNPKPRTPNFPYLCRKKRQWTT"
     gene            172..753
                     /gene="scpB"
                     /locus_tag="PK198_23835"
     CDS             172..753
                     /gene="scpB"
                     /locus_tag="PK198_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRF41854.1"
                     /translation="MPQKTTVDYLTQHVESLIFTAEQPISVAEIQSTLEEAFGLKFQE
                     TELNQIVTDLQARYNADTYAFEVVEIDGGFQLMTKAAYHNTVGAWLRQTMSRKLSRAA
                     LETLSIVAYKQPVSKSEMERIRGVNCDYALQKLLEKELVSIAGRSDGPGRPLLYATGQ
                     KFMDYFGLKSIKDLPQLKEFKEPESSIGEPETA"
     gene            759..1277
                     /locus_tag="PK198_23840"
     CDS             759..1277
                     /locus_tag="PK198_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRF41855.1"
                     /translation="METEKTLVNRVAESGLITLHLEKFFPDGEVAVFDLKDYLFMELI
                     LKEKDFREALKNHDWEQYRGKILLTYCSADAIIPLWAYMLVTAYAAPHALDVFQGDEE
                     AYYKAAFLRALAQLDGAQYEQQRVVVKGCSDKPVPPAAYVEITRKLQPYAQSIMFGEP
                     CSTVPVFKRPRI"
     gene            1274..2947
                     /locus_tag="PK198_23845"
     CDS             1274..2947
                     /locus_tag="PK198_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRF41856.1"
                     /translation="MTNKEIAKTFQLLADIMELHGENSFKIRSYQNAYIALRKLETPL
                     SEMPREEIDAVKGVGKAIGDKIQELLASGQMAALEKYRAMTPPGVQEILAIPGIGPKK
                     TLTIWKDLGAESVGELLYAVNENRLIELKGFGAKTQEDIRQKLEYYQRSLGHYRYANL
                     EAEAKSLLAALQSALPEGNIALAGDVARYCNTLETIDFVAIHHSDWPSVLQQAGLSAD
                     APDASPIIARTASGVPLRIFLCNPEEFGSKQFRYTAGQAFMEAFVAAFPGVDFRGLPT
                     EEAVFQKVGLPFIAPELRDWPAAIELAQKNALPELLSEKDIRGVIHAHSTYSDGLHSL
                     SDMAEYARTQGYEYLGITDHSKSAFYAQGLEPERVLMQWREVDALNAAYATTGHKFRI
                     FKGIESDILNDGSLDYDAELLAGFDFIIASVHSNLRMDEAKATRRLLTAIENPHTSIL
                     GHPTGRLLLSRAGYPIDHRLLIDACAANGVAIELNANPYRLDLDWTWIPYAMEKGVLI
                     AVNPDAHSREGIHDIHFGVCSARKGGLTAAACLNVLDAEEFGAWAGRKG"
     assembly_gap    3083..3092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3093..3679
                     /locus_tag="PK198_23850"
     CDS             <3093..3679
                     /locus_tag="PK198_23850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRF41857.1"
                     /translation="LANELGFDFSEADNEQLKGVSRMESLEIILKWGGVALSEEQKIE
                     WADRKNEWYKALISNMTPDEILPGVPEFLAQLQSNGIRIALGSASKNALPVIESIGLG
                     PVFEVVIDGTQTTLSKPDPQVFRLGAQRLGLKPYECIVFEDAESGIEAALNGRFYAVG
                     IGRPEVLGKAHLVIPGFVGLQWEEVVKAMGGEND"
BASE COUNT          890 a         1110 c          990 g          708 t
ORIGIN      
        1 cccggaacgc gaatttttaa cgcgaatttt agcgaaggtc gcgaatttcc gcgaatgcga
       61 gggaaaatgt gatttgtacg ccctgcatgg cttgcagcca gcagccatta gccggcagcc
      121 gttcccacct gaacgaaacc caaaacctcg aaccccaaat ttcccctatc tttgccgcaa
      181 aaaacgacag tggactacct gacgcaacat gtggaaagcc tgatcttcac cgccgagcag
      241 cccatttcgg tggccgagat acagagcact ttggaagaag cattcggact gaaatttcag
      301 gaaaccgaac tgaatcagat cgttacggat ttgcaggccc gctacaacgc cgatacctac
      361 gcctttgaag tggtggaaat tgacggcggt tttcaactca tgaccaaagc cgcctatcac
      421 aacaccgtgg gcgcctggct gcggcaaacc atgtcgcgca agctctcccg cgccgccctc
      481 gaaaccctct ccatcgtggc ctacaagcag cccgtttcca agtcggaaat ggagcgcatt
      541 cgcggcgtca actgcgatta cgccctgcaa aaactattgg aaaaagagct tgtttctatt
      601 gcgggtcgca gcgacggccc cggcagaccg cttctgtatg ccaccggcca gaaatttatg
      661 gactacttcg gcctcaagag catcaaagac ctgccgcaac tcaaagaatt taaagaaccg
      721 gaaagctcta tcggcgaacc cgaaactgcc tgaatcgtat ggaaacagaa aaaacattgg
      781 tcaatcgcgt ggcggaaagc ggcctcatca ccctgcacct ggagaagttt ttccccgacg
      841 gagaagtcgc tgtgttcgac ctcaaagact acctctttat ggagttgatt ctcaaggaaa
      901 aagacttccg cgaggcgctc aaaaatcacg attgggaaca gtaccggggc aaaatcctgc
      961 tcacctactg ctctgccgat gccatcattc cgctgtgggc ctacatgctc gtcaccgcct
     1021 acgccgcgcc ccatgccctc gatgtgtttc agggcgatga ggaggcgtat tacaaagccg
     1081 ctttcctgcg cgcattggcc caactggacg gcgcacagta cgagcagcag cgcgtcgtcg
     1141 tcaaagggtg cagcgacaaa cccgtgccgc ccgccgcgta tgtggagatc acccgcaaac
     1201 tgcagcccta cgcccagagc atcatgttcg gcgagccttg ctctacggtg ccggtgttta
     1261 aacggccccg gatatgacga acaaagagat agccaaaacc ttccaattgc tggccgacat
     1321 catggagttg cacggcgaaa acagcttcaa aatacgctcc taccagaatg cgtacatcgc
     1381 tttgcgcaaa ttggaaacgc ccctctcgga aatgccccgc gaggaaatag acgccgtcaa
     1441 aggagtgggc aaggccatcg gcgataaaat ccaggaattg ctggcctccg ggcaaatggc
     1501 tgccttggaa aaataccgcg ccatgacccc gcccggcgtg caggaaatat tggccattcc
     1561 cggcatcggg ccgaaaaaaa cactcaccat ctggaaagac ctcggcgctg aaagcgtggg
     1621 cgaactgctc tacgccgtca acgaaaaccg cctcatcgaa ctcaagggct ttggcgcaaa
     1681 aacgcaggaa gacatccgcc aaaaactcga atactaccag cgttccctcg gccattatcg
     1741 ctacgccaac ctcgaagcgg aggcaaaatc gctcctcgcg gccctgcaat cggccctgcc
     1801 ggagggcaac atcgcgctcg ccggagacgt ggcccgctat tgcaatacgc tggaaaccat
     1861 agacttcgtg gcaatccatc acagcgactg gccctccgtt ttgcaacagg ccggactgag
     1921 cgccgatgcg cccgatgcct cgccgatcat tgcgcgcacc gcgtcgggag tgcccctgcg
     1981 cattttcctg tgcaacccgg aggagttcgg ttccaagcag tttcgctaca ccgccggaca
     2041 ggcattcatg gaggcttttg tggcggcttt tcccggcgtt gattttcgcg gattgcccac
     2101 cgaagaggct gtttttcaaa aggttggact gccgttcatc gcccccgaac tgcgcgactg
     2161 gcccgccgcc attgaactgg cgcagaagaa cgccctgccc gaactgcttt ctgaaaaaga
     2221 cattcgcggc gtcatccatg cgcactctac ttacagcgac ggtctgcact cgctcagcga
     2281 catggccgaa tacgcccgca cgcagggcta cgaatacctc ggcatcaccg accactccaa
     2341 atcggccttc tacgcacagg gcctcgagcc ggaacgcgtg ctcatgcagt ggcgcgaggt
     2401 ggatgcgctc aacgccgctt atgccacaac cggacataag tttcgcattt tcaaaggaat
     2461 agaaagcgac attttgaacg acggcagctt ggactacgac gccgaactgc tcgccggatt
     2521 cgattttatc atcgcatcgg tacacagcaa cctgcgcatg gacgaggcca aagccacccg
     2581 ccgcctgctc acggccatcg aaaacccgca taccagcatt ctggggcacc ctaccggcag
     2641 gctgctgttg tcgcgggccg gctacccgat tgaccaccgc ctcctcatag acgcctgcgc
     2701 cgccaacggc gtagccatag aactgaacgc caacccttat cgcctcgatt tagactggac
     2761 ctggattccg tacgccatgg aaaagggcgt cctcattgcc gtcaatccag atgcgcacag
     2821 ccgcgaaggc atacacgata tacatttcgg tgtgtgctcg gcacgcaaag gcggtctcac
     2881 ggcagcggcc tgtttgaatg tgctggatgc ggaggagttt ggggcgtggg cagggagaaa
     2941 ggggtaggat ttgccctttg ccctttgccc gctgctcctt acctttgccc cgcacaaaac
     3001 gaaagcatga tcaaagcctg tattttcgac ctcgacggag tgatagtaga taccgccaag
     3061 tatcattttc aggcttggcg ccnnnnnnnn nngattggcc aacgaactcg gtttcgactt
     3121 ttcggaagcc gacaacgaac aactcaaggg cgtcagtcgc atggaatcgc tggaaatcat
     3181 tctgaaatgg ggcggcgtcg cgctctccga ggagcaaaaa atcgagtggg ccgaccgcaa
     3241 aaacgagtgg tacaaagccc tcatcagcaa catgacgccc gatgaaatac tgcccggtgt
     3301 acccgaattt ctagcccaat tgcaatcaaa cggcatccgc atcgccctcg gctcggccag
     3361 caaaaatgcc ctgcccgtga tcgaaagcat cggactgggg ccggtttttg aagtcgtcat
     3421 agacggcaca caaaccaccc tcagcaaacc cgatccgcag gttttcagac tgggggcgca
     3481 gcggctgggg ctgaaacctt acgagtgcat cgtttttgaa gatgctgaaa gcggcattga
     3541 ggcggcgctg aacggtagat tttacgccgt aggcattggg cggcccgaag tgctgggcaa
     3601 ggcgcacctg gtcattccgg gtttcgtcgg cttgcaatgg gaggaggtag tgaaggcgat
     3661 ggggggggag aatgactgac ttgtttagcg catccaagag ccacaagc
//
LOCUS       DAORLF010000456         3666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13770_length_3666_cov_1.546940, whole genome shotgun sequence.
ACCESSION   DAORLF010000456 DAORLF010000000
VERSION     DAORLF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_3666_cov_1.546940"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PK198_23855"
     CDS             <1..1357
                     /locus_tag="PK198_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41858.1"
                     /translation="RQISDAHLGPAGYLPLIALSPVWEGHFHNALVGTGVSMVTLYYL
                     RRFMRFWQDMLGGFKADNIQISKELAKFYGEVLECLHSSAPLLATGISDKDRKAVLDR
                     LGMAASDYRQGIYQNGFSGDKSSVTLEGLKDFARVSLEFIEHSIRANQRPDKLYHAYN
                     LMTAGSNTVSVAHLSEMLEGQVAVLSSGYLSAEAAMEVLDALKNSALFREDQYSYILY
                     PNKNLPGFLHKNTIPASDAQHSDLLQQLVADGNAALVEQDVEGALHFNGYFKNAKDVE
                     LALSALEAGSYKDLVAKDRQRVLDIFEKVFHHKAFTGRSGTFFSYEGLGSIYWHMVSK
                     LLLAVQECCLHAIESKAEPKVIGRLLDHYYEIEAGIGVHKSPVLYGAFPTDPYSHTPL
                     HRGAQQPGMTGQVKEEILTRWGELGLRMRQGEVHFDPVLLDAAELPEGGELRFTLSTS
                     R"
     gene            1426..1581
                     /locus_tag="PK198_23860"
     CDS             1426..1581
                     /locus_tag="PK198_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41859.1"
                     /translation="MYSHSDSDHIAVHYADGSTVGIAGHVLEHRISSLVFQRTAEVER
                     LEVFLKS"
     gene            1586..>2564
                     /locus_tag="PK198_23865"
     CDS             1586..>2564
                     /locus_tag="PK198_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41860.1"
                     /translation="MSATRARLKVMQSNLRFGRNIAGRVLLLLLGGIILLIVSCSPSR
                     QPVEKTAADILGNPDYQAISFGSYRELSRDVQPTIPQLKEDLKILSAMGIKLLRTYNV
                     YYDEASNLLKAISDLKKEDPAFEMYLMLGAWIDCKNAWTELPPDHNMESEKNAGEIDR
                     AVALAQQYPDIVKIIAVGNEAMVKWAASYYVQPGVILKWVNHLQNLKKPGQLPETLWI
                     TSSDNFASWGGGESEYHVEDLNQLIKAVDFISMHTYPMHDTHYNPDFWGVPDSEEGLS
                     DLEKTEAAMLRAKGYAMSQYESVVSYMKSLGVNKPVHIGETGWSTQSNER"
     assembly_gap    2565..2634
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2635..2921
                     /locus_tag="PK198_23870"
     CDS             <2635..2921
                     /locus_tag="PK198_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41861.1"
                     /translation="NAAGITCFYFEAFDEQWKDAANPGGSENHFGLFNLQAQAKYMIW
                     DLVDAGVFKGLTRDGQPITKTFNGDKDAVMKTVKAPPTTEETKAYSPQNN"
     assembly_gap    2938..3054
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3055..>3274
                     /locus_tag="PK198_23875"
     CDS             <3055..>3274
                     /locus_tag="PK198_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 30"
                     /protein_id="HRF41862.1"
                     /translation="FLIVENPLEINILPDETFQTVTGFGGSFTEASCYLLNRLSEANR
                     AKVLEAYFGESGARYSLTRTHINSCDFSL"
     assembly_gap    3275..3297
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3298..>3666
                     /locus_tag="PK198_23880"
     CDS             <3298..>3666
                     /locus_tag="PK198_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 30"
                     /protein_id="HRF41863.1"
                     /translation="PVAGDMKLDSFSIKEDMDDIVPIIKAARAISKDGFKIISSPWTA
                     PPWMKDNKDWRGGKLLPEYYDTWALFFSKYLNAYQAQGIDIWGLTVENEPLGNDGNWD
                     SMHYTPEEMTQFVKNHLGPKL"
BASE COUNT          857 a          901 c          945 g          753 t
ORIGIN      
        1 gcggcagatc agcgacgcgc atctggggcc ggccggctac ctgccgctca tcgccctctc
       61 gccggtgtgg gagggccatt tccacaacgc gctggtgggc accggggttt cgatggtgac
      121 gctgtactac ctgcggcgat tcatgcgatt ctggcaggat atgctgggcg gtttcaaggc
      181 tgataatata cagatttcca aagagttggc gaagttttac ggtgaggtgc tggagtgcct
      241 gcacagctcg gctcccttat tggccaccgg catcagcgac aaagaccgga aggctgtttt
      301 ggaccgtttg ggaatggccg cttccgatta ccggcaaggt atttatcaaa acggattcag
      361 cggagacaaa agcagcgtca ctttggaagg acttaaagac ttcgctcgtg tgtcgcttga
      421 atttatagaa cactccattc gggccaacca acgccccgac aaactctacc acgcctacaa
      481 tctgatgacg gcgggttcca atacggtttc ggtggcccat ctcagcgaga tgctggaggg
      541 gcaggtggcc gtcctgagtt cgggctacct gagtgcagag gccgcgatgg aagtgttgga
      601 cgccttgaaa aacagcgcgc ttttccggga ggatcagtac agttacatcc tgtatcccaa
      661 taaaaatttg ccgggatttt tgcacaaaaa caccatcccc gcatcggatg cacagcactc
      721 ggacctcctc caacaattag tggctgacgg caatgccgct cttgtggagc aagacgtgga
      781 gggcgccctg cacttcaacg gctatttcaa aaatgccaaa gatgtggaac tggcgctgag
      841 tgctttagaa gccggctcct acaaagattt ggttgcgaag gaccgccaac gggtactgga
      901 tatttttgaa aaagtattcc accataaggc tttcacaggg cgttcgggca ccttcttttc
      961 ttacgaaggg ctgggttcga tctactggca catggtgtcc aaactcttgc tggcagtgca
     1021 ggaatgttgc ctccacgcca tcgaaagcaa ggccgagcct aaggtaatcg gccggctttt
     1081 ggatcattat tatgagatag aagccggtat tggcgtacac aaatcgccgg tattgtacgg
     1141 cgcgtttccc accgatccgt attcgcacac gcccttacac cggggcgcac agcagccggg
     1201 catgaccggg caggtgaagg aggagatcct cacccgctgg ggcgagctgg gcctgcgcat
     1261 gcgccagggt gaagtgcact tcgacccggt gctgctggat gcggccgaac tgcccgaggg
     1321 tggcgaactg cgcttcacct tgagtacatc gcggtaagcg gcgagcgcaa gacccttgct
     1381 ttgcaggcgg gccaattggg gcttacatat tgtcagattc ccattgtgta ttcccattcc
     1441 gattcagatc acatcgccgt tcattacgcc gatggcagca cggtaggcat agccggccat
     1501 gttctggagc atcgcatcag cagcctggtc ttccagagaa ctgcggaggt agaacgattg
     1561 gaggtgtttt taaaatcgta acaaaatgag cgcaacaaga gcaaggctaa aagtaatgca
     1621 aagtaatctc cgctttgggc ggaatatagc aggacgtgtg ttgttgctgc ttttgggcgg
     1681 gataattttg ctcatcgttt cgtgcagccc aagtcgtcag cccgttgaaa aaactgctgc
     1741 cgatatattg ggcaatcccg actatcaggc catttctttc ggctcttatc gcgagctgtc
     1801 gcgggatgtg cagcccacca ttccgcagtt gaaggaagac ctgaagatac tgtcggcgat
     1861 gggcatcaaa ctgctcagga cgtacaacgt gtattacgat gaagcctcca acttactgaa
     1921 ggccatcagc gatctgaaaa aagaagaccc cgcctttgaa atgtacctca tgctgggcgc
     1981 ctggatagac tgcaaaaacg cctggaccga actgccgccc gaccataaca tggaaagcga
     2041 aaagaacgcc ggagaaatag accgcgcggt ggctttggcc caacaatacc cggatattgt
     2101 aaaaatcatt gccgtgggaa atgaggccat ggtgaaatgg gccgccagtt attatgtgca
     2161 gcccggcgtc atcctcaagt gggtcaatca cctgcaaaac ctcaaaaaac caggccaact
     2221 gcccgaaaca ctgtggatca caagttctga taacttcgcc tcctggggcg gcggcgagag
     2281 cgagtatcat gtggaagatt tgaatcaatt gatcaaagcc gttgacttca tttccatgca
     2341 cacctacccc atgcacgaca cccattacaa ccccgatttc tggggtgtgc cggatagcga
     2401 ggagggcttg tcggatttgg aaaaaactga ggcggccatg ctccgggcca aaggctacgc
     2461 catgagccaa tacgaaagcg tggtgagcta tatgaaaagc ctcggcgtga acaaacccgt
     2521 tcacattggc gaaaccggct ggtccacgca gtccaacgaa cgctnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccaatg
     2641 cggccggcat cacctgtttt tattttgaag cctttgacga gcaatggaaa gacgcggcca
     2701 atccgggcgg ttcggaaaac catttcggcc tgttcaattt gcaggctcag gccaaatata
     2761 tgatttggga cctggtggat gccggcgttt tcaaggggct tacccgcgac gggcagccca
     2821 tcaccaagac cttcaacggc gacaaggatg ctgttatgaa gacggtgaag gcgcctccca
     2881 ccacagagga aaccaaggca tacagtccac aaaataatta gcaatatgaa aaagctgnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttct
     3061 aattgtcgaa aatccgctgg agatcaacat attacccgat gaaaccttcc agacggtcac
     3121 cggtttcggc ggctcgttta ccgaagcctc ctgctacttg cttaaccggc tgagcgaggc
     3181 caacagggcc aaagttctgg aggcttattt tggagaatcc ggcgcccgat actctctgac
     3241 gcgcactcac atcaactcct gtgatttctc attgnnnnnn nnnnnnnnnn nnnnnnnccg
     3301 gtagccggcg atatgaaatt ggacagtttt tctataaaag aagacatgga cgacatcgtg
     3361 ccgatcatca aagcggcccg tgccatttcc aaagacggct tcaaaatcat ctcctctccc
     3421 tggacggcgc cgccctggat gaaagataac aaagactggc gcggcggtaa attgctgccc
     3481 gaatattacg atacctgggc gctgtttttt tctaaatacc tgaatgccta ccaagcgcaa
     3541 ggtattgata tttgggggct tacggtagaa aatgagccat tgggcaacga cggcaactgg
     3601 gacagtatgc actacacgcc ggaggagatg acccaatttg tgaaaaacca cctggggccg
     3661 aaattg
//
LOCUS       DAORLF010000457         3663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13795_length_3663_cov_1.703991, whole genome shotgun sequence.
ACCESSION   DAORLF010000457 DAORLF010000000
VERSION     DAORLF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13795_length_3663_cov_1.703991"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PK198_23885"
     CDS             complement(<1..672)
                     /locus_tag="PK198_23885"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF41864.1"
                     /translation="MDFTAFWEREIEAHSMKEVSKRAVRQTLNKIREMQKQVFFDDLT
                     FAFIQEFDRRLRKQALSLNTINKHHKRLKTFISLAVKYGHLKPEQNPYGRFILKGEEP
                     ERPCLSEEELERLTFSADEKHLERHRDFLLLQCYTGLRFSDVAHLTPSKMSWSSEGIT
                     LHFKAGKTGKPLICPLYLFFPDAEGNSRPGAIVERYFRPLVALEEAGAQLMPLKIENQ
                     PLNRSL"
     assembly_gap    740..816
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<817..999)
                     /locus_tag="PK198_23890"
     CDS             complement(<817..999)
                     /locus_tag="PK198_23890"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HRF41865.1"
                     /translation="MAYKKISFAFVFNRKNVLDDKGRALVQIKAYQEGKNRYFSTEVY
                     LEPKHWDERNKRVKPAH"
     gene            complement(1208..1281)
                     /locus_tag="PK198_23895"
     tRNA            complement(1208..1281)
                     /locus_tag="PK198_23895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Arg,seq:tct)
     gene            1399..1629
                     /locus_tag="PK198_23900"
     CDS             1399..1629
                     /locus_tag="PK198_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41866.1"
                     /translation="MLPKRGKGKCFFQSRQQFGWAFWFLAVPKKDQACAGETQARIES
                     DFRLQEKLASEREMITASTLSFTMQKSNPRIN"
     gene            complement(1633..>2422)
                     /locus_tag="PK198_23905"
     CDS             complement(1633..>2422)
                     /locus_tag="PK198_23905"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HRF41867.1"
                     /translation="LTDAVSPIMEKLERVAAVVNDVPMLMDALPPLVRIVRYGNVRRT
                     DSEAVGLWLRRIIPRIAAGLPAACLQLDEEPADEMLARLWETHRHIMLLSEEALSAAW
                     LGALEQLSGTDAAHPLLQGSATRIAFDRRLKEETAARNAFSLALSAGNEPVHAARWVE
                     GFLHGSGLLLIHHPPLWRLLDEWVAALPEAGFMAVLPLLRRAFASFSVAERQKMLAMA
                     QDGISVAATTENTGIDPDRAAVVAPVLSLLLGFPLRINADLTPV"
     assembly_gap    2423..2434
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2435..>3663)
                     /locus_tag="PK198_23910"
     CDS             complement(<2435..>3663)
                     /locus_tag="PK198_23910"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HRF41868.1"
                     /translation="HYAAQHGIPVKAMDLPAGTQWMLNSAEGDAPVLVETPSDPENQA
                     IAADPLGYLAALAGYPDGERWWDSVLEQIPAGSDVFEAVAEMMRALRETLQREESPET
                     LLREAFMRKTIREAATAGFQNIAVVCGAWHVSALEPWQQIKAAKDTALLRGRKKVKTE
                     TAWVPWTYERLAYESGYGAGVLSPAWYELLFEMPAGAVTQWMVRAARLLRGEDIDVSG
                     AHAIEAVRLAEALAALRGKPLAGVEDMASAALSVFVQGNEQVFDLVRRRLVIGEVVGA
                     VPASLSAIPLQRDFEKEVRSAHLSKEYAAVDEVSKQLDLRVPGNLKASLLLHRLGLLG
                     IPWGRLHEQQGPSGSTFHEVWRLKWLPDYALQIVRAGSWGNTIESAAVQCVLERSRQT
                     EQLPDLTRLTEEVLRAA"
BASE COUNT          779 a         1038 c          953 g          804 t
ORIGIN      
        1 taggctcctg ttgagcggct gattttcaat tttgagcggc atgagctggg cgcctgcctc
       61 ttccagggcg accaatggcc ggaagtaccg ctccacgatc gcgccgggcc tgctgttgcc
      121 ctcggcatcc gggaaaaaaa ggtacaacgg gcaaataagc ggcttcccgg tctttccggc
      181 tttgaagtgc aaggtgatgc cctcagacga ccaagacatt tttgagggcg tcaaatgtgc
      241 cacgtcggaa aaccgcaggc cggtgtagca ttgcagcaac aaaaaatctc tgtgccgctc
      301 caggtgtttc tcatcggcgg aaaaagtcag ccgctccaat tcttcttcgc tcaggcatgg
      361 tctttccggc tcctctcctt tgagtatgaa acgcccgtac ggattttgct ccggtttgag
      421 gtgcccatac ttgacggcca gcgaaatgaa ggttttcagg cgcttgtggt gtttgttgat
      481 cgtattgagc gacaaagcct gtttcctcag tcggcggtca aactcctgga taaaagcaaa
      541 ggtgaggtcg tcaaagaaaa cctgcttttg catttccctt attttgttaa gggtttgccg
      601 tacggcccgc tttgaaacct ccttcatgct gtgcgcctcg atctcccgtt cccagaaagc
      661 ggtaaagtcc acctctgttt gtgcaggctg ctcttttgca gcctttagct gatctattga
      721 gaagcggcca tttttggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt gcgggcttta cccgcttgtt
      841 gcgttcgtcc cagtgtttcg gctccagata tacctctgtt gagaagtacc ggtttttgcc
      901 ttcctggtat gcttttatct gtaccaatgc cctacctttg tcgtcgagca cgtttttacg
      961 gttgaacaca aacgcgaaac tgatcttttt gtaagccata tttgaaccgg tttagatgcc
     1021 aaagtaaagt ctttgagcca acccggtaca caaatgggta caaaaaacaa acaggtatag
     1081 cgcaaagtgt tgaaaaacaa cacattttgt tgtgttttta cctgccgtaa cacgttgatt
     1141 ttcaaacgtg tctgataaat tgggagctat caggaaattt cctgataact cccgaaacaa
     1201 acttttgtga tcccgacagg actcgaacct gtgaccgtct gcttagaagg cagatgctct
     1261 atccaactga gctacgggac caatatttca atcaaacttg attcctttac atacatcaaa
     1321 tacttacctt atgcaggcga accggcaggc aggtatccaa ctgagctacg ggaccaatat
     1381 ttcaactcaa aacacgcctt gcttccaaag cgcggcaaag gtaaatgttt tttccaatcc
     1441 cgtcaacagt tcggatgggc attttggttt ctagctgttc ctaaaaaaga tcaggcctgc
     1501 gccggggaga cgcaggcccg aatcgaaagc gactttagat tgcaggaaaa attggcatca
     1561 gaaagagaaa tgataacagc aagcaccctt tctttcacga tgcaaaagtc caacccacgc
     1621 atcaattgaa ttttaaaccg gggttaaatc ggcattaatt ctcaacggaa atcccagcaa
     1681 taagctcagc accggcgcca ccacggcagc cctgtccggg tctatacctg tattttccgt
     1741 agtagcagca acagagattc catcttgggc catagccagc attttttgtc gctccgcaac
     1801 cgaaaacgat gcaaacgccc gccgcaacag gggcaatacg gccatgaacc cggcttcggg
     1861 caatgcggcc acccactcgt cgagcaggcg ccacaagggt gggtggtgta tgagcaacag
     1921 accgctgcca tgtaaaaaac cttccaccca gcgggccgca tgtaccggct cgttgcccgc
     1981 cgacaaagcc agcgaaaacg cgtttcttgc cgccgtttct tccttcagcc gccggtcgaa
     2041 agcgatgcgc gtggcgctgc cctgcaacag cggatgcgcc gcgtcggtac cgctcaattg
     2101 ttccaacgcc cccagccatg ccgccgaaag cgcctcctcg ctcagcaaca tgatgtgccg
     2161 gtgggtttcc caaagccgag ccaacatctc gtcagccggt tcttcgtcca attgcaggca
     2221 ggccgccggc aatcccgccg caatgcgggg aataatgcgg cgcaaccaca gccccacggc
     2281 ttccgaatcg gtgcggcgca cattgccgta gcgcacaatg cgcaccaagg gcggcagcgc
     2341 gtccatgagc atgggcacgt cattgacgac ggcggccacg cgttccaatt tttccatgat
     2401 gggcgaaacg gcgtcggtga ggnnnnnnnn nnnnggcggc acgcagcact tcttccgtca
     2461 ggcgggtgag atcagggagt tgctcggttt ggcggctgcg ttccagcacg cattgcactg
     2521 cggcgctttc tatggtgttg ccccaactcc cggcgcgtac gatttgcagc gcgtagtcgg
     2581 gcagccattt cagtcgccag acttcatgaa aagtgctgcc ggaggggcct tgctgctcgt
     2641 ggagccgccc ccagggaatg cccaacaaac ccaagcgatg caagagtagc gaggctttca
     2701 ggttgccggg cacgcggagg tccaattgtt tgctcacctc gtccacggcg gcgtattctt
     2761 tgctcaggtg ggccgagcga acttcttttt caaaatccct ttgcagggga atagcgctca
     2821 gcgaggccgg aaccgcgccc accacctcgc cgatgaccaa gcgacggcgc accagatcaa
     2881 acacctgctc attgccctgc acaaaaaccg acagcgccgc cgatgccatg tcttccactc
     2941 ctgcaagggg cttgccgcgc aaggctgcta aggcttccgc caaacgcacc gcctcgatgg
     3001 catgcgcccc cgaaacgtca atgtcttcgc cgcgcaggag ccgcgccgcc cgcaccatcc
     3061 actgggtgac ggccccggcg ggcatctcaa aaagcagttc gtaccaggcc ggagacaaaa
     3121 caccggcgcc gtagccgctt tcgtaggcca atcgttcgta ggtccagggt acccaggcgg
     3181 tttctgtttt cacttttttt ctgccgcgca gcagagcggt gtctttcgcg gctttgatct
     3241 gctgccaggg ttcgagtgcg gacacatgcc aggcaccgca cacgacggct atgttttgga
     3301 agcctgccgt tgccgcttcg cggatggtct ttcgcataaa agcctcgcgc agaagcgttt
     3361 cggggctttc ttcgcgctgc aaggtttcgc ggagcgcgcg catcatttcg gctacggcct
     3421 cgaatacgtc gctgccggca gggatttgtt ccaacaccga atcccaccag cgctcaccgt
     3481 cggggtagcc ggccaaagcg gcgaggtatc ccagtggatc ggctgcaatg gcttggtttt
     3541 cagggtcgga aggggtttcc accaaaacag gagcatcgcc ttccgcgctg ttgagcatcc
     3601 actgcgtgcc ggcgggcagg tccatcgctt tgacaggaat gccatgttga gcggcgtagt
     3661 gca
//
LOCUS       DAORLF010000458         3650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13870_length_3650_cov_2.591655, whole genome shotgun sequence.
ACCESSION   DAORLF010000458 DAORLF010000000
VERSION     DAORLF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13870_length_3650_cov_2.591655"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1399)
                     /locus_tag="PK198_23915"
     CDS             complement(245..1399)
                     /locus_tag="PK198_23915"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HRF41869.1"
                     /translation="MAGNENRLKGLVSALRGVKDPRIERSKLYPLNEVLFLCVSAVIS
                     GYESWDEIVDFGEEKLSWLMGFLPFENGIPSHDTLNRVVSLIDYRAFEKSFIDWATMG
                     ISLPNGVQICIDGKKLRRSATKLEQQTPHSQGGKSAVHLVHAWCNELELCLGQYKTKS
                     KSNEITAIPALLDLLEIGGSVITIDAMGCQKKIAEKIVEKKGEYIFGLKGNQENLSAS
                     VTEVFKDEKHSSQAQQEISHSKGHGRKEERICRVLSADILPESAQISEWKNLESIIEI
                     CSKREIMSTGKIEIEKRYYISSLRESAPNFNALIRSHWGIENKLHWTMDVLFGEDQSR
                     KRIKNAAENFSTIRKIVLNLIKASPEKISVNRKRKKCALSDQYRESVMRI"
     gene            complement(1430..>2229)
                     /locus_tag="PK198_23920"
     CDS             complement(1430..>2229)
                     /locus_tag="PK198_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41870.1"
                     /translation="EAGGRLPEVLYLRIRDGTWMPLFLSYPTHTPTCPLEYSQNTAIF
                     VKKQPVMLKNLLLLLLAATGARLMAQTWPNCDLPPASYLLNGNNVTALIQNNGMLFNS
                     TDTAAGFRVYLPSADAETHTLRRASIWIGGLDPVGNMFLATGGDHGDSSVMRFKPGKI
                     NVATQTPYPGSCENFNNIWRVERDAIQRHIFDFLDNGVIDHPEPSLMAWPGRGNPHFE
                     AYNGFPFPSDTPPAMMAPFWDADGDRMYNPLRGDYPGPTHQNRMKKS"
     assembly_gap    2230..2250
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2427..2936
                     /locus_tag="PK198_23925"
     CDS             2427..2936
                     /locus_tag="PK198_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41871.1"
                     /translation="MKLRTCILLLMAASALFSHCCKDDSSTHTRVEIFGLQSFEWEGP
                     GICAVRPGSVVLESSALVTNDDIVSYNDSQALFEFREPSAQNIRNLSPRTPFALTLDG
                     EVLFIGVVMQNIMSSTCFESITMENLTYGPRRAKLELGYPSGINPSLEDKRKDAALLA
                     ALRSQGKLR"
     gene            complement(2955..>3650)
                     /locus_tag="PK198_23930"
     CDS             complement(2955..>3650)
                     /locus_tag="PK198_23930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRF41872.1"
                     /translation="IGRLDKDSQGLIFLTNNGDMVNKILRAGNKHEKEYVVTVNRPLT
                     EEALKGMSKGVPMLGTYTKKCKIVQEGVKVFRITLIQGLNRQIRRMCEHYDYEVVKLE
                     RVRIMNITLKGLPLGEWRDLTEEEMAHLHELTKDSSSAPPTPAKPKRSSAGKKPFVSK
                     SGTSGAYKGTADRNKKGKSAEASPFGKPGKPASGKRSGGGPKPKSGKGAPGGYAGRTG
                     TGSRSTKPKSRGR"
BASE COUNT          809 a          919 c          876 g         1025 t
ORIGIN      
        1 ccttctttct aaccgcaata aaaacttcct gcgttcagct ttttcacagg agtccattct
       61 ggattcgatt gtgcaaagct aatggaagtt tgacataaaa tcaaccgctc agtaaaaaac
      121 tttttcgccg gttgggcctg ttggtgcgat ggcccctgca ataccggcag gcaggcggcc
      181 acggccctga gacgtctgcc tactattgga taagggagcg gcgggggggg gcagggccga
      241 cgcatcaaat tcgcatgact gactctcggt actgatcgga cagggcgcac ttttttcgtt
      301 ttcgattaac cgatattttt tcagggctgg ccttgatcag atttaaaact attttacgaa
      361 tggtagaaaa gttttccgct gcatttttta ttcgcttacg actttgatct tcgccaaaaa
      421 gaacatccat cgtccaatga agtttatttt caatacccca atgagaacga attaatgcgt
      481 taaaattagg ggcgctttcc cgaaggctac taatgtaata tctcttctct atttcaattt
      541 ttccagtact catgatttca cgctttgagc aaatttcaat gattgattcc aggtttttcc
      601 attcagatat ttgagcagat tcagggagaa tatccgcact taaaaccctg caaatccgct
      661 cttccttgcg cccatgtccc ttagaatgac tgatctcttg ctgcgcttgt gagctgtgtt
      721 tttcatcctt aaatacttct gttacactcg cacttaaatt ctcttggttt ccttttaatc
      781 cgaaaatata ttcccctttt ttttcgacta tcttttctgc tatctttttt tggcacccca
      841 tcgcatctat tgtgattaca ctgccgccta tttctaataa atccaataag gcaggaattg
      901 ctgttatctc attggatttg ctcttcgtct tatattgacc cagacagagt tctaattcgt
      961 tacaccatgc gtgtaccaaa tgtaccgccg atttaccccc ttgtgagtgg ggtgtctgct
     1021 gctctaattt cgtcgcactc cgacgtaatt ttttaccgtc tatgcatatt tgaactccat
     1081 tgggaagact tatgcccatc gtcgcccaat caatgaaact cttctcaaat gcacggtaat
     1141 ctatcaagga taccacccta tttaatgtgt catgcgaagg tatcccgttt tcaaagggca
     1201 aaaagcccat caaccatgat aacttttctt ctccaaaatc aacaatctca tcccagctct
     1261 catatccact tatcacggcg ctgacgcata aaaacagaac ctcgttcaat ggatacagct
     1321 tactcctctc tattcttggg tcttttaccc ctcgaagggc tgataccaac ccttttaaac
     1381 gattctcatt gcctgccatc tttccttttg gtcagaaaga tactttcttt caggatttct
     1441 tcatcctatt ttgatgcgtc ggccctgggt agtcaccccg cagcggattg tacatcctgt
     1501 ctccatctgc atcccaaaaa ggagccatca tcgccggagg ggtatcgctt gggaaaggga
     1561 agccgttgta tgcctcaaag tgcggattgc ctctgcccgg ccaggccatg agcgatggct
     1621 ccggatgatc tatgacgccg ttgtcgagga agtcgaaaat gtgccgttga atggcgtccc
     1681 gttctacccg ccatatatta ttgaagtttt cacaggatcc gggataaggc gtctgggtag
     1741 caacgttgat ttttccgggt ttgaagcgca tcacggaact atcgccatga tcgccgccgg
     1801 ttgccagaaa catgttgccg accggatcaa gtcctccgat ccaaatggat gcccggcgaa
     1861 gcgtgtgcgt ttcggcatcg gcggagggca gatacacccg aaagcccgcg gcggtgtcgg
     1921 tgctgttgaa caacataccg ttgttttgga tgagtgcggt cacattattg ccgttgagga
     1981 gatagctcgc gggcgggaga tcgcagttcg gccaggtttg agccataagg cgggcgccgg
     2041 tagcggcgag caggaggagg aggaggtttt tgagcataac aggttgtttt ttaacaaaaa
     2101 tagctgtgtt ttgggagtac tccagagggc aagtgggcgt atgtgttggg tacgacaaaa
     2161 aaaggggcat ccaagtgccg tccctgatgc gcaagtataa aacttcaggc agcctgcccc
     2221 ccgcttcaan nnnnnnnnnn nnnnnnnnnn gaaatcgtat caatattatc gccatgaaac
     2281 ctctccaaat gaccggcaga ccctcgcaaa gaaggctggt cggacagcat tcacggcgta
     2341 tttcgggtgc gtctggggta ttacttcccg gttggaagag ggcaaaaaaa tgaaaaccat
     2401 ttcacaccat caaaaacgaa tgatcaatga aacttcgcac ttgtattttg ctgcttatgg
     2461 cagcatcggc cttgttttca cattgctgta aagatgattc cagtacccat actcgcgtgg
     2521 aaatatttgg tttacagagt tttgaatggg aggggccggg catttgcgcc gtccgtcccg
     2581 gctcggtggt attggagtct tcagctcttg tgactaacga cgacatcgtt tcttacaacg
     2641 attcccaagc cctttttgaa tttagggaac cctcggccca aaacatccga aacctttcgc
     2701 cgcgaactcc tttcgccctg acgctggacg gcgaagtgct cttcatcgga gtggtgatgc
     2761 agaacatcat gagctccact tgtttcgagt ccattacgat ggaaaatctg acttatggcc
     2821 ctcgtcgcgc caaactggaa ctgggctacc cctcaggcat caacccttcg ctggaagaca
     2881 aacgaaaaga cgcggcgctt ttggcggcgc tgaggagcca agggaagttg aggtgaatgg
     2941 tattttactg cgtactacct cccccttgac ttaggcttgg tgcttcggct gccggttccg
     3001 gtacggcctg cgtacccgcc tggagcgccc ttgccggatt taggtttcgg gccaccgccg
     3061 ctgcgtttcc cggatgctgg tttgcccggt ttgccgaaag gcgaggcttc ggcacttttc
     3121 ccttttttgt tgcggtcggc agttccttta taagcccccg aagtgcctga ttttgagaca
     3181 aaaggctttt tacccgcgct gctccgtttg ggtttggcgg gcgtcggcgg ggccgaggaa
     3241 gagtctttgg tcagttcgtg cagatgagcc atttcttcct cggtgagatc gcgccactcg
     3301 cccagaggca gccctttgag tgtgatgttc ataatgcgaa cgcgctccag cttgaccacc
     3361 tcatagtcgt agtgttcgca cattcgacga atctggcgat tgagtccctg tatgagggtg
     3421 atgcgaaaga ccttcacgcc ctcctgcacg atcttgcact ttttcgtgta ggttcccaac
     3481 atgggcacgc ctttggacat gcctttgagg gcttcttcgg tgaggggtct gttcacggtg
     3541 acgacgtact ctttctcatg tttgttgccg gcgcgcagta ttttattgac catgtctccg
     3601 ttgttggtca aaaaaataag cccttgcgag tccttgtcca agcggccgat
//
LOCUS       DAORLF010000459         3639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13915_length_3639_cov_2.108538, whole genome shotgun sequence.
ACCESSION   DAORLF010000459 DAORLF010000000
VERSION     DAORLF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13915_length_3639_cov_2.108538"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK198_23935"
     CDS             <1..520
                     /locus_tag="PK198_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRF41873.1"
                     /translation="IGELATMEVRDKKGISKQMQTKIWGVNVSSTDVDITAISVLKVG
                     FNLKEFLDISVNSSAGAVVVTLPQPVILSHEVHPKVEKLDIGWLREVKTEDFNKNIDL
                     LRSEFRREALESDIMEKSKKQARELLDTMMGPLVKSLGSRYKLVVRFKENPQSRENTG
                     RTAAGEVVRLEE"
     gene            complement(650..1630)
                     /locus_tag="PK198_23940"
     CDS             complement(650..1630)
                     /locus_tag="PK198_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41874.1"
                     /translation="MKSSVADALKQARMGQPVQFKNLTVFPLFEEANTPFTYLTMRSA
                     LEKGWVEITELNEGGSVPVLKVINRSDALVLLLDGEELRGAKQNRIVNTSILLPAQSE
                     TAIPVSCTERGRWHYNSRQFSESGYMMSSMSRNSKNARMYCRMMAMKDEPAEASESHR
                     AYDAGQGEVWSEISALHGKLRTSSHSDAMSDAYEQRSGDINAYVAAFPLQPGQNGMVA
                     LLNGKPISADMLSQPDAYADIHEKLIRSLAVELIGSTQADSTVLSPEDMSVEAYKFLH
                     TLHEGHEAAFQPVGLGEDLRYDIAQAGGAALTYQDALVHLNIYSKEKMAL"
     gene            complement(2297..2749)
                     /locus_tag="PK198_23945"
     CDS             complement(2297..2749)
                     /locus_tag="PK198_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRF41875.1"
                     /translation="MLELHLDRFKDELRLKREGDTRLIWDPIRRKWLVLQPEEMVRQL
                     LLQYLLTERGYNRNRIRMEKGLQVNALDRRCDLLVFDAQVQPFLLAECKAPHVKIGQE
                     VFEQIAWYNTTLQVPYLLVTNGIESHCCKVDYAARSYEFLNEVPAHDA"
     assembly_gap    2824..2839
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2840..>3639)
                     /locus_tag="PK198_23950"
     CDS             complement(<2840..>3639)
                     /locus_tag="PK198_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41876.1"
                     /translation="FANHYDGIHTTPTTLTLSANFGGVSQPVFFLNNTNQNVSSGATA
                     TARTNIQNQVNTAWATTPVVQSGIRAIHNSAAQTLNVTAKSRFFQSASGEYYLGIYLV
                     FKEFVGFQSPIGNNAEHKEMLRSHLTANAFGEQIANGAVAAGVETEVSGQISIAGLDP
                     TKLRVVTIIWKKEGTTYRVTNANETDDFLAPSSANDLWAAQQQLKILPNIITESARIE
                     LQITESSLPWRMELVNTSGQVMETIFQGTLAGGTHTFSVERGTRPAGL"
BASE COUNT          824 a          909 c          998 g          892 t
ORIGIN      
        1 catcggcgaa ttggccacga tggaggtgcg cgacaaaaaa ggcatcagca agcaaatgca
       61 aaccaaaatc tggggcgtca atgtgtcttc taccgacgtg gacatcaccg ccatcagcgt
      121 tctcaaagtg ggcttcaacc tcaaggaatt tctcgatatt tccgtcaatt cctctgccgg
      181 ggcggtcgtc gtcacgctgc cgcagccggt catcctctcg cacgaggtac accccaaagt
      241 tgaaaaattg gacatcggtt ggctgcgcga ggtgaaaacc gaggatttta ataaaaacat
      301 agacctgctg cgctcggagt ttcgccgcga agccttagaa agcgacatca tggaaaagtc
      361 gaaaaagcag gcccgcgaac tcttggatac catgatgggg ccattggtga aatcgctcgg
      421 ctcgcgctat aaattggtgg tgcgcttcaa ggaaaatccg caaagccgcg aaaatactgg
      481 tcggacggca gccggtgaag tggtgaggct ggaggagtga gggggcggtt ggagcggctg
      541 ctggctgcga gctgcaagct gcaagcttgg tggggcgttc gtaaaaaagt attcttagga
      601 gaagggcggc gaccacatcc gtggcgtagt cgccgcctgc aacgtttact cacaaagcca
      661 ttttctcttt ggagtagata ttgagatgca ctaaggcatc ctggtaggtg agcgcggcgc
      721 cgccggcctg cgcgatgtcg tagcggaggt cttcgccgag gcccacaggc tggaaggctg
      781 cttcatggcc ctcgtgcaaa gtgtgcagga acttgtacgc ttccacgctc atgtcttcgg
      841 gcgacagcac agtagaatcg gcctgagtac tgcctatcaa ctcaacggct aagctcctga
      901 tgagtttttc gtggatatcg gcgtaggcat cgggttgcga aagcatatcg gcgctgatgg
      961 gcttgccatt gagcagggcc accatgccgt tctgaccggg ttgcagcgga aaagcagcta
     1021 catacgcgtt gatgtcgccg gagcgctgtt cgtaggcatc actcatggcg tcggagtggg
     1081 aggaggtgcg cagtttgccg tgcaaggcgc tgatttcgct ccacacttcg ccctgcccgg
     1141 cgtcatacgc gcggtggctt tcagatgcct cggcaggctc atccttcatg gccatcatgc
     1201 ggcaatacat gcgcgcgttc ttgctgtttc tggacatgct cgacatcata tagcccgatt
     1261 cgctgaactg ccggctgttg tagtgccagc ggccccgttc ggtacaactg acgggtatgg
     1321 ccgtttcgct ttgggcgggc aagagtatgg acgtattgac gattcggttt tgcttggcgc
     1381 cccgcagttc ttcgccatcc aaaagcagca ccaatgcgtc gcttcggttg atgactttca
     1441 aaacgggtac ggagcctcct tcattcagtt cggttatttc aacccatcct ttttctaagg
     1501 cacttcgcat ggtcaggtag gtaaacggcg tgttcgcttc ctcaaaaaga gggaatacag
     1561 taaggttttt gaattgcacg ggttgcccca tacgcgcctg cttcagggcg tctgctaccg
     1621 aagatttcat acgataaaaa attttgagat ggaaaaaccc ttcaaccatt tggaggggtg
     1681 gaaaagttcc cgatgcgggg catggggtga aatggttgat tagccggggc cgccattcag
     1741 cttggcttta agaaaatgta aatctgtttt tgctgcttat gtagaagagt ttctaatctg
     1801 aaacaaatgc catttcccac ttctttttgc aaaatagtgt tcgatataaa gccccgtatc
     1861 aatgccttga agaattacat tcatggtatc atttgatatt ctagtggaag aataggaaat
     1921 ctccgaatcc cagtcttccc ggtcgaaaga aacgtatttc actacgatat gcctgtcttc
     1981 caccacttct tcatcagtaa ggacaacgtg aagaggagcg tcaatccttt gtagttgaaa
     2041 aatcgtatct tgaatgaatc gagaaaaaaa tatttcaaaa ccgctcgcgc cgagattcca
     2101 tctcgcgtga atattatgcg ggttctaccc gcttgcagaa cactcttttt taatcatttg
     2161 acctcacgcg gcaggcataa tgttcgacca ctccgaggtc atcacgcatt tttaatttgc
     2221 tgatctacaa atatttgatc tctccgagat catttgcaaa atccggaatg actcatgttt
     2281 gtggttcaaa aagttatcag gcatcatgcg ccggcacctc gttcaaaaac tcataagacc
     2341 tcgccgcata atccaccttg caacagtggg attctattcc attggtaacc aataggtaag
     2401 gaacttgcag ggtggtattg taccaggcaa tttgctcaaa tacctcctgc ccgattttca
     2461 catgcggagc cttgcactcg gccagtagaa aaggctgcac ctgcgcatcg aacaccaaga
     2521 ggtcgcagcg ccggtccaat gcgttgacct gcagtccctt ttccatgcga atgcgattgc
     2581 ggttgtagcc ccgttccgtc agcaggtatt gcagcagcag ttggcgcacc atttcttcgg
     2641 gttgcagcac caaccacttg cggcgtatcg ggtcccagat caagcgggtg tcgccttcgc
     2701 gtttcaggcg caactcgtct ttgaaccggt ctaaatgtag ttccagcatg ggtgttgtaa
     2761 tcagcgcaac accacccgtt gcgacgtgga gcgcaggccg ttgctcagtc tgacaaaata
     2821 caannnnnnn nnnnnnnnnc aaacccgccg ggcgagtgcc gcgctctacg ctaaaagtgt
     2881 gtgttccacc ggccagtgta ccctggaaaa tcgtttccat cacctggccg gaagtattga
     2941 ccaattccat gcgccagggt aggctgcttt ccgtgatctg caactctatg cgggccgatt
     3001 cggtgatgat attgggaaga attttcaatt gctgttgggc ggcccagagg tcattggccg
     3061 aactgggcgc cagaaaatcg tcggtttcgt tggcgttcgt cacgcggtag gtagtgccct
     3121 cttttttcca gatgatggtc acaacgcgca gttttgtcgg gtcgagaccg gcaatggaaa
     3181 tctggccact gacctcggtt tcgacgccgg cggctactgc tccgttggct atttgctctc
     3241 cgaaggcatt ggctgtcagg tggctgcgca gcatttcctt gtgttcggcg ttgttgccta
     3301 taggtgactg aaatccaaca aactccttga aaaccaaata gatgccgagg tagtactcgc
     3361 cggaagccga ctggaagaaa cgcgatttgg ccgtcacgtt cagcgtttgg gcggcgctgt
     3421 tgtgaatggc acgaatgccg ctttgcacta ccggagtggt agcccaggcg gtgttcacct
     3481 gattttggat attggtgcgg gccgtggcgg tggcgccgct ggagacgttc tgattggtat
     3541 tgttcagaaa aaagacaggc tggctcacgc cgccgaaatt ggcagacaaa gtgagggtcg
     3601 tcggcgtagt gtggatgccg tcatagtgat tggcaaaaa
//
LOCUS       DAORLF010000460         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13947_length_3633_cov_1.882057, whole genome shotgun sequence.
ACCESSION   DAORLF010000460 DAORLF010000000
VERSION     DAORLF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13947_length_3633_cov_1.882057"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..>711
                     /locus_tag="PK198_23955"
     CDS             465..>711
                     /locus_tag="PK198_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41877.1"
                     /translation="MMKRFTLLTLLLLPALAFAQAPQAFSYQAIATDNLGKELTNRAI
                     GIRASVLQGSPSGAAVWVETHAVSTDEFGLFTLSIGQG"
     assembly_gap    712..729
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <730..>1681
                     /locus_tag="PK198_23960"
     CDS             <730..>1681
                     /locus_tag="PK198_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41878.1"
                     /translation="GTLPAFANIAWEAGPYFLRIDMDPDGGTAYNFVGTNPLLSVPYA
                     LYSQKSGSADRAVFADSSAVAGLAQRALLADSAAVAHQAQRAILADSAIVAGRAQSAL
                     LADSATVAGRAQRAMLADSSIVAHHAEEARHAVRADTAGFSELAVLAANAFVADYANN
                     AGTANSAAYANNAGIAQFATLAGSAVDDFDKDSLNEIQTLSISRDTIRISNGNFVALN
                     AVSPFNAPGATFDFPEGIDHAFFLYVFNSYTVPAGRNFYITASQSEVRLPGVGNAIGR
                     HFTNPSQPLIPAGTTVSNCRCTGFLVNQTSQINPVVIVLQP"
     assembly_gap    1682..1706
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1707..1897
                     /locus_tag="PK198_23965"
     CDS             <1707..1897
                     /locus_tag="PK198_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41879.1"
                     /translation="VPIGRYLVIKSGTTATMPVSYDGTVIDFFSSNLKAMIAPSGVTL
                     RNPNAEEIILTGYLKNNN"
     gene            1904..3133
                     /locus_tag="PK198_23970"
     CDS             1904..3133
                     /locus_tag="PK198_23970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF41880.1"
                     /translation="MSHIFNLQRWWPALCLLLLAGSSAQAQGIRRSVIGSTGGSALVG
                     ATGYRLRSTVAQPPNAGTIANNTNYLRQGFQQPTTCAASPQALMEALALSPDNCGSLY
                     VFNYLDQPDPTTQFFWTFGLGAQPQASYELAPGEVGYLAPGVKMIILEVLTDDCRNTD
                     TLLLDVPVIPMTAVANVSDLWCREEEDGGISLFLTGGTEPYSVAWSNGDAGIDIGGLS
                     PGDYAYTITDANGCLRNGTATVSSPDSLRATEQIRDESCLGSLNGSIALSVSGGTAPY
                     SYQWSEGSSSATAGNLAKGSYSVTLSDSHDCPQYLEFEVGRLCEGLTFYDVFTPNGDG
                     KNDRWIIEGIEQFPGSRLSVYDRWGGLIYTAQGYANDWEGRRNDDSILPMGAYFYLIH
                     LGDPADTVLKGSVTIIR"
     gene            3145..>3633
                     /locus_tag="PK198_23975"
     CDS             3145..>3633
                     /locus_tag="PK198_23975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF41881.1"
                     /translation="MKHLYLFSYLLMIFKKLRAASFELRAASKLRRSILFLVVFIVGS
                     MALQAQQYPLFTNYLLNQYGFNPATTGMATDLQVNLYYRKQWTGFPDAPVTRIAAVRS
                     RLRRVPFAVGGYFFKDEAGALRRSVGTLMLSYTQRIGEKTTISAGFAGGFHQLRLMDD
                     FTV"
BASE COUNT          755 a         1143 c          934 g          758 t
ORIGIN      
        1 tatctaatcc tttggcctaa agaaaatgaa catttatttt tgccccacta cttagctaaa
       61 caaactgctc ccttttttct taagccgaca gccgcgaaga aaatcccctt tttcaaaaaa
      121 aactttccct tccctcttgc gctgcgttga aaaaagctac aacttgcgcc ccgaaagact
      181 atcagccatg ctttcaaccg gcccattaac cgattcgccg ggcagtaacc actcgcaaag
      241 caagcgagtg acgtacactt tttgactgca acattccggt ttacgctcat aggattacta
      301 tctactctat cgtcgcctgc cgcacaggcg gggtactgtg ttttgctgtg ttcgcacccg
      361 cctttcaacg cgaacgccgg cagaaccgga atccctattg ctgtgcgtcg gggagtggaa
      421 gattttgtaa accaacattt tgtaatccaa taagacgcaa ccgaatgatg aaacgcttta
      481 cgctccttac tttgctgctg ctgccggcac tcgccttcgc gcaagccccg caggctttca
      541 gctaccaggc catcgccacc gacaatctcg gcaaagaact caccaaccgc gccatcggca
      601 tacgcgcctc tgttttgcag ggcagcccct ccggcgccgc cgtctgggta gaaacccacg
      661 ccgtgagcac cgacgagttt ggcctcttta ccctctccat cggacaggga annnnnnnnn
      721 nnnnnnnnnc ggcacgctgc ctgctttcgc caatatcgcc tgggaggccg ggccgtactt
      781 cctccgcatt gacatggacc ccgacggcgg aacagcctac aactttgtgg gaaccaaccc
      841 attgctctcc gtgccttacg cgctgtacag ccaaaaatcg ggcagcgccg acagagccgt
      901 ttttgccgac agctcggcag tagcgggcct tgcccaacgc gctttgctgg ccgacagcgc
      961 cgccgtagcc catcaggctc aacgcgccat actggccgac agcgccatcg tggccggccg
     1021 cgctcagtcc gcattattgg ccgacagcgc caccgtggcg ggccgcgctc aacgggcgat
     1081 gctggccgac agctccatcg tggcgcatca cgctgaggaa gcccggcacg ccgtccgcgc
     1141 cgatacggcc ggtttttcgg aattggccgt gctggccgcc aacgcttttg tagcggatta
     1201 tgccaacaac gccggcaccg ccaactcggc tgcctacgcc aacaatgccg gtattgcgca
     1261 atttgccacc ctggccggtt cggcagtgga cgacttcgac aaggactcgc tcaacgagat
     1321 tcaaacgctt tccatatcga gagataccat ccggatttcc aatggtaact ttgtggcgct
     1381 caatgcggtc agtcccttca atgccccggg cgccactttc gattttcccg aaggcattga
     1441 ccacgccttt ttcctttatg ttttcaacag ctacaccgtg ccggcgggca ggaattttta
     1501 cattacggca tcgcaaagcg aggtgcggct gcccggcgta ggcaatgcca tcgggcggca
     1561 ttttaccaat ccctcacagc cgctcattcc ggcaggaacc accgtctcga actgtcgctg
     1621 caccggcttt ttggtcaatc aaacctcgca gatcaacccg gtggtcattg tgttgcaacc
     1681 cnnnnnnnnn nnnnnnnnnn nnnnnnaggt gcccatcggg cggtatttgg tcatcaaaag
     1741 cggcacgaca gctaccatgc cggtatcgta tgacggcacg gtgattgact ttttcagcag
     1801 caatctcaaa gccatgatcg cgccgtcggg ggttactttg cgcaatccca acgcagagga
     1861 aatcatcctg accggctatt tgaaaaacaa caactgatac gccatgagcc acattttcaa
     1921 ccttcaacgt tggtggccgg cgttgtgtct gttgttgctt gccggcagtt ccgcgcaagc
     1981 gcagggcatc cgccgcagcg tcatcggcag tacaggcggt tcggcactcg taggcgccac
     2041 gggttatcgc ctgcgcagca cggtggcaca gccgcccaac gcaggcacca ttgccaacaa
     2101 taccaactac ctccgccagg gattccagca acccactacc tgcgccgctt cgccacaggc
     2161 tttgatggaa gcccttgccc tgtcacccga caactgcggc agcctctatg ttttcaacta
     2221 tttggaccaa cccgatccga cgacacagtt tttctggacc ttcggactcg gcgcgcagcc
     2281 gcaggcctct tacgaactcg ctcccggaga agtgggctac ctggcacccg gcgtcaaaat
     2341 gatcatcctc gaagtgctga ccgacgactg ccgcaacacc gataccctgc tgctcgacgt
     2401 gccggtcatc cccatgacgg cggtggccaa tgtgagcgac ctgtggtgcc gcgaagagga
     2461 agacggcggc atcagtctgt ttctcaccgg cggtaccgag ccgtactccg ttgcctggtc
     2521 gaacggcgat gcgggcattg acatcggcgg actctcgccc ggagactacg cctacaccat
     2581 caccgacgcc aacggctgcc tgcgcaacgg aaccgccacc gtgagcagcc ccgacagcct
     2641 gcgcgccaca gagcaaattc gcgacgagag ttgcctcggc agtctcaacg gtagcatcgc
     2701 cctgtcggtc agcggaggca cagcgcccta ttcctaccaa tggagcgagg gcagcagcag
     2761 cgccaccgcc ggcaatcttg ccaaaggcag ctactcggtg accctcagcg acagccacga
     2821 ctgcccgcaa tacttagagt tcgaggtcgg caggctctgc gaaggactga cgttttacga
     2881 tgtcttcacg cccaacggcg acggcaaaaa cgaccgctgg atcatcgagg gcatcgagca
     2941 gtttcccggc agccggctca gtgtttacga ccgttggggc ggcctgattt acacagcgca
     3001 aggctatgcc aacgactggg agggccgccg caacgacgat tcgatactgc cgatgggcgc
     3061 ttatttctac ctcatacacc tcggagaccc ggccgacacc gtgctgaaag gctccgtcac
     3121 catcatccgt taaaatccat gagcatgaaa catttatact tattttccta tctgttgatg
     3181 attttcaaaa agctgcgagc tgcgagcttc gagctgcgcg ccgcgagcaa gctgaggcgc
     3241 agtatcctgt ttttagtggt tttcattgtc ggcagcatgg cgttgcaggc gcagcaatac
     3301 cctttgttta ccaattattt gctcaatcaa tatggcttca atccggctac gaccggcatg
     3361 gccaccgatc tgcaagtgaa cctgtactat cgcaaacaat ggacgggttt ccccgatgcg
     3421 cccgtcacgc gtattgccgc cgtgaggagt cggttgcggc gcgtaccttt tgccgtgggc
     3481 gggtattttt tcaaagacga ggccggcgca ttgcggcgca gcgtcggcac tttgatgttg
     3541 tcttataccc aacgcatcgg cgaaaaaacc accatttcgg cgggctttgc cggcggtttt
     3601 caccaattgc ggctcatgga cgacttcaca gtg
//
LOCUS       DAORLF010000461         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_13997_length_3623_cov_2.208520, whole genome shotgun sequence.
ACCESSION   DAORLF010000461 DAORLF010000000
VERSION     DAORLF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13997_length_3623_cov_2.208520"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3055
                     /locus_tag="PK198_23980"
     CDS             <1..3055
                     /locus_tag="PK198_23980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41882.1"
                     /translation="WGTALTLAQVQAEMECGADPSNNNLLIYFPFDQNIPGGNNAGMV
                     AVTDEGPGGGVTNGMLSGFALNGNTSNWVARGRDLLPVCNVSAFDWLEGDRMDNRAGR
                     TKVFDSHLFTAGTHKLQPGGPDFPVFTRRRPDGSIVWRTRLNIPGYINDFIYTDEGCY
                     LLVGHSPSFTGDNRSFIARIAENGVLNWLHTYNIQEREAFNRIIRSSNPSNPNFPYAV
                     CGIAKPAGTSTDDILLFTLNNAGSIGWMRRIGTTSSDDEFRMDLLDVNGTYVLTGLRI
                     GAGGLRGVLFKTDNAGVQLTSSGEYNNVFSIQTAAQTGSDLLVAGQLPGGNGAYIARL
                     NGAGVQVWARKFPDIDNFTRVVVRPGGQVYALGFRRPGAPYRNIIVKVLDTPNGGMVQ
                     WMKRSDLSDDSAWVGADLVHYSGDMFFYTDGRTKNPGAGFGNYDIAAVMDRIDLLPCP
                     YSTDSLHHESFTMPFAALPTLSNALIAPPVPVITNPWSPVSYEGIRLCNNPCTCRFEN
                     LGFSRKVVAGIPIIPGNWPVTCDTVPVNLPCPAQLSPILFRGRLTCNGDNCEFTGLSW
                     EIRDPNNNMIASGTSPTSQFSIPITLAMVQTPGVYSIHLSGSCGGMTECSCIVRFRIP
                     ECPRPCDCNEPAFTQDVHQAFNRVQSSGCKFTFTPKALTPCDAVQWRVAKSSDLIFTV
                     FANSAGNNSVMYTFPASEQYVVCYTVTRTVPGIGTVCSASRCVLLDVNCSLLFQNDDN
                     AIQLRSQSCAGSIVQNGGFTEGAEAGSFNDGGSIANWERSAGDPTVVLEQGAQDPNFV
                     RLHGSSGFADVLYQNEVNVPMHSNIDFVTAYRPLPGLIRPGTQLVARISTEQQDSIFC
                     SDNTNCRELFRIDIPQNAPHEWFVVGTYDSIPFTGRYVTLHVENPFADDDPGLQSAVE
                     IDNVCLRKFQFVNVKTTGPVARLQIWPNPTSGEINIQLPVWEGRTDARVLVSDLTGRI
                     VTEKRTTGTDTIETIQLGKLPAGIYLVQIQEASGRVVAVERIIKQ"
     gene            3228..>3623
                     /locus_tag="PK198_23985"
     CDS             3228..>3623
                     /locus_tag="PK198_23985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41883.1"
                     /translation="MRSIICWGALMFGLNICLSAQAPGYLGKRIFIKPDIAYAPALAH
                     PTAGNRGGNTYGEQGTRLGLNTKYGLQAGYAFSRRGVLAAEANYMATGMSLTTYTPAV
                     RYEGRMDEHYLFYNLRGPELGLALQTYNPL"
BASE COUNT          861 a         1014 c          917 g          831 t
ORIGIN      
        1 ctggggtact gcgctcacgc tcgctcaggt acaagccgaa atggaatgtg gcgctgaccc
       61 ctcgaacaac aacctgctca tttatttccc ctttgaccaa aatattcccg gcggaaacaa
      121 cgcaggcatg gttgcagtaa cagatgaagg gccgggcggc ggcgtcacca atggtatgct
      181 cagtgggttt gcgctcaacg gcaacacctc caactgggtg gccaggggcc gcgatctttt
      241 acccgtatgc aatgtgagcg cttttgactg gctggaggga gaccgtatgg acaatcgggc
      301 cggacgaacc aaagtgtttg acagtcacct ctttacagcc ggcacgcaca agctccaacc
      361 gggtggtccc gattttccgg tctttacccg ccggcggccc gacggcagca tcgtgtggcg
      421 cacccggctc aatatacccg gatatatcaa cgacttcata tataccgacg aagggtgcta
      481 cctgttggtg ggccattcac cttcttttac cggcgataac cgcagcttca tagcccgcat
      541 tgccgaaaac ggtgttctta attggctaca tacctacaac atacaggagc gagaggcatt
      601 caaccgcatc attcgcagtt ccaacccttc caaccctaat tttccatacg ccgtatgtgg
      661 aattgcaaaa cctgcgggaa cctcaacgga tgacatttta cttttcacgc tcaacaatgc
      721 aggcagcatc ggctggatgc gacgcatcgg aactacctcc agcgacgacg aattcagaat
      781 ggaccttttg gatgtgaatg gaacctatgt tttaaccggt ctcagaattg gcgccggcgg
      841 tttgcgaggc gtattgttca aaaccgacaa tgccggcgta caacttacat catcaggcga
      901 atacaacaat gtgttcagta ttcagactgc tgcacaaaca ggtagcgatt tgctggtagc
      961 cggccaattg ccgggtggca acggcgcgta catcgcccgc ctcaatggag cgggagtaca
     1021 ggtttgggcg cgaaaatttc ccgacattga caactttacc cgcgtggtag tgcgccccgg
     1081 cgggcaggta tatgcacttg gcttccggcg gcccggcgcg ccttatcgca acatcatcgt
     1141 caaagtattg gatacgccca atggcggcat ggtgcaatgg atgaagcgct ccgatctgag
     1201 tgatgattcc gcctgggtag gcgctgattt ggtacactat tccggtgata tgtttttcta
     1261 caccgatggg cgcaccaaaa atccgggggc agggttcgga aattacgaca tagccgccgt
     1321 aatggatcgc attgaccttt tgccctgccc gtacagcaca gattctttgc accacgagtc
     1381 ttttacaatg ccttttgcag cactacctac actgtccaat gcactgatag cgccacctgt
     1441 gcctgtcatc accaatcctt ggtcgcctgt atcttatgaa ggtattaggc tttgcaataa
     1501 cccatgtacc tgcaggtttg agaatctggg attttcccgc aaagtagtcg ccgggattcc
     1561 gattataccc ggcaactggc ccgtcacttg cgatacggtg ccggtaaatt tgccctgccc
     1621 ggcacaactg agtcctatct tgttcagagg gcgcctgact tgcaatggcg acaactgcga
     1681 attcacgggc ttgagttggg aaatacgaga tcccaacaac aacatgatag catccggcac
     1741 atcgcctacc tcacaattca gtattcctat cacattggca atggtgcaaa cgccgggcgt
     1801 atattccatc catttatcag ggagttgtgg cggcatgacg gaatgttcct gcattgtcag
     1861 gttccggata cccgaatgtc cgaggccatg cgattgcaat gaaccagctt tcacgcaaga
     1921 tgtacatcag gcattcaatc gggttcagtc gtccgggtgc aaattcacat ttacacccaa
     1981 agccctcact ccctgcgacg ccgtgcaatg gagggtggcc aagtcaagcg atttgatatt
     2041 taccgtattc gccaattctg cgggtaacaa ttcagttatg tacaccttcc ctgccagcga
     2101 gcaatatgta gtgtgctaca ccgtgacgcg cacagtaccc ggcatcggca cagtatgctc
     2161 ggccagccga tgcgtactgt tggatgtcaa ttgcagcctg ctcttccaaa atgacgacaa
     2221 tgcgatacag ttgcgttctc aatcatgcgc cggctcaatc gttcaaaacg gcggcttcac
     2281 agaaggagca gaggccggca gtttcaacga tggcggaagt attgccaact gggaacgcag
     2341 cgccggagac ccgaccgtgg tgctggagca gggcgctcaa gaccctaatt ttgtacgcct
     2401 gcacggcagc agcggcttcg ctgatgtgct gtaccaaaat gaagtgaacg tccctatgca
     2461 cagcaacatt gattttgtga cggcatacag gccattgcca ggtcttatca ggcccggcac
     2521 ccaattggtg gcccgcattt cgacggaaca gcaagacagt atcttctgct ccgacaatac
     2581 gaactgccgg gagcttttca gaattgacat tccacaaaat gcacctcatg agtggtttgt
     2641 tgtgggtact tatgattcta ttccgtttac cggccggtat gtgaccctgc atgtagagaa
     2701 cccctttgcc gatgatgacc caggtcttca atcagcggta gaaattgaca acgtatgtct
     2761 ccggaagttc cagtttgtca atgtcaaaac aaccggcccg gtggcacgcc tgcaaatctg
     2821 gcccaacccg acatccggcg aaataaacat acaactgccc gtatgggagg gccgtactga
     2881 tgcccgtgtt ttggtatcgg acttgacggg gcgcatcgtg acagagaaaa gaacgaccgg
     2941 cacagatacc attgaaacga tacaattggg gaaacttccc gccggcattt atctggtgca
     3001 aattcaggag gcatccggca gggtagtcgc ggttgaaaga atcatcaaac aataacctaa
     3061 aaccacgggg gcgaccggct ttcgtccgtt gcccccctgt tttcttaccc tcaaccttcc
     3121 aatataatga aaaacagatt tgtgaatgcc ggaacggtat tcgtattcta tctctttgcc
     3181 tttcaggcca taaccaccac cctctcacac caaaaccaca tcaacctatg agatcaatta
     3241 tctgttgggg cgcccttatg ttcggcctca acatctgcct cagcgctcag gcgcccggct
     3301 atttgggcaa gcgcattttt atcaaacccg acatcgccta tgctccggca ttggctcacc
     3361 ctactgccgg caaccggggc ggcaatacct acggagaaca aggcacgcgc ttgggtctca
     3421 ataccaaata tggcctgcag gcaggctatg cgttctctcg gcgcggagtg ctggccgccg
     3481 aagccaacta tatggccacc ggcatgtcgc tcaccactta tacgcccgct gtgcgctacg
     3541 aaggccggat ggacgagcac tacctgtttt acaacttgcg cgggccggaa ctgggcttgg
     3601 cgctgcaaac ctacaaccct ttg
//
LOCUS       DAORLF010000462         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14156_length_3588_cov_2.363431, whole genome shotgun sequence.
ACCESSION   DAORLF010000462 DAORLF010000000
VERSION     DAORLF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14156_length_3588_cov_2.363431"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PK198_23990"
     CDS             <1..964
                     /locus_tag="PK198_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41884.1"
                     /translation="FFNANFINAKIDMEKGEGPSIAQKYAVRAYPTFLFVDSDGELVH
                     RTLGYQEGGMFIENAKAALDPQRRISALNARYAGGDRSPEFLRKYTEARAQMMDGSHG
                     KIAEEYLQTQKDWGTEENMEFLFQYAEDPDSPMFAYIFKERAKFEAAYGEEMVRGKIQ
                     QAAFSSVMKMGDLEPEAAVKKMDAFFAEKCPDIAPKLSAQTAMTVYKMMGQSDLYAKT
                     AVKYYDQYPSNNFLELNEAAWGFYENVEDKALLQKALGWGLKSVELQNEYFNNDTVAA
                     LYYKLGNKKQATKYAKKAIAMAKKNGEDASETEALLAKMKGKKK"
     gene            967..2004
                     /gene="thiL"
                     /locus_tag="PK198_23995"
     CDS             967..2004
                     /gene="thiL"
                     /locus_tag="PK198_23995"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRF41885.1"
                     /translation="MSDQISRTDVNTLGEFGLIEHLTRNFELRNPSSLKGINDDAAVI
                     DNGNFLTVVSTDLLIEGIHFDLMYTPLKHLGYKAVVVNLSDIYAMNARPKQITVSIAI
                     SNRFSVEALDELYAGIRAACERYGVDLVGGDTSSSNKGLLISVTAIGQGEADKLTYRN
                     TARVGDLVCITGNLGAAYLGLQILEREKQLFLSDPKIQPNLEDQSYLIGRQLKPEARK
                     EMIEMFAQQQLVPTAMMDISDGLSSDLMHICRQSGVGAYIEESGVPIHPEAQQMAIKF
                     GLDPITVALSGGEDYELLFTIDPNDVEKVKYLPDIYIAGEIVPAEDGLKLHTKGGNIH
                     AMTAQGWTHFG"
     gene            2055..3044
                     /locus_tag="PK198_24000"
     CDS             2055..3044
                     /locus_tag="PK198_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF41886.1"
                     /translation="MKYLIGLAIIGPILLLSACKSDKPADKAPADAPAAEVSAPRFDR
                     DSAYFFVEKQVSFGPRVPNTAAHTACKDWYVERLRAYGAEVIEQSFRPTAYDGTVLQS
                     VNIIARYNPAAPQRIVLAAHWDSRPFADSPINTERTKDPVLGADDGASGVGVLLEVAR
                     QLQAQPLQNIGIDLVFFDAEDYGDMGGNNDKSWGLGAQHWSRNLHVPANQFKYGILLD
                     MVGAKGARFAREQVSMRFAPQVMDKVWKLAQGLGYGNYFVDEDGGAVTDDHYFVNNIA
                     GIPMIDIINRPTTTQTGFGDYWHTHNDDMDVIDPRTLRAVGQVLLELIYKEDQ"
     gene            3041..>3588
                     /gene="hisH"
                     /locus_tag="PK198_24005"
     CDS             3041..>3588
                     /gene="hisH"
                     /locus_tag="PK198_24005"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRF41887.1"
                     /translation="MIAIVQYNAGNIRSVQNALQRLGRPSIVTYVPEEIERASHVIFP
                     GVGEAGSAMKYLRDKGLDVVLQNLKQPVLGVCLGLQLLCRHTEEGDTRCLGIFDAEVK
                     RFPPQDKVPHIGWNNCAALSGRLLQSIDPQQDFYFVHSYYAQTCEHTAAVCDYILPFS
                     AALEKDNFFAVQFHPEKSAGAG"
BASE COUNT          898 a         1028 c          937 g          725 t
ORIGIN      
        1 ttttttcaac gccaatttca tcaacgccaa aattgacatg gaaaaaggcg aaggcccttc
       61 cattgctcaa aaatacgctg tacgcgctta tcccaccttc ctgtttgtgg acagcgacgg
      121 cgagttggta caccgcacgc tgggctatca ggagggaggc atgtttatcg aaaacgcaaa
      181 ggcagcactc gatccgcagc gccgcatctc tgcgctcaat gcccgctatg ccggcggtga
      241 tcgcagtccg gaatttctgc gcaaatacac cgaagcccgc gcccaaatga tggacggcag
      301 ccacggaaaa attgcagaag aatatctcca aacccagaaa gactggggca ctgaggaaaa
      361 catggagttt ctgtttcaat acgccgaaga ccccgattcg cctatgtttg cttatatttt
      421 caaggagcgc gcaaagtttg aagctgctta cggagaagaa atggtgcgag gtaaaatcca
      481 gcaggccgct ttcagcagtg ttatgaaaat gggcgatctg gaaccggagg ccgcggtgaa
      541 aaaaatggat gcgttttttg cagaaaaatg ccccgacata gcccccaagc tgagcgcgca
      601 aaccgctatg actgtgtata aaatgatggg ccaaagcgac ttgtacgcca aaaccgccgt
      661 caagtattac gatcagtacc cgtccaacaa cttcctcgaa ttgaacgagg cagcctgggg
      721 tttttacgaa aatgtggaag acaaagcctt gctgcaaaaa gcattgggct ggggtctgaa
      781 atcggtggaa ctgcaaaacg agtatttcaa caacgacacg gtggccgcct tgtattacaa
      841 actcggcaac aaaaaacagg ccacgaaata cgccaaaaaa gccattgcca tggccaagaa
      901 aaatggcgaa gatgcttcgg aaactgaagc gctgctggcg aaaatgaaag ggaagaaaaa
      961 atagaaatgt ctgatcaaat ttccagaacc gacgtgaata ccctcggtga gtttggactc
     1021 atcgagcacc tgacgcgcaa cttcgagctg cggaacccct cctcgctcaa aggcatcaac
     1081 gacgatgcgg ccgttattga caacggcaat ttcctcaccg tcgtctctac cgacctgctc
     1141 atcgagggca ttcacttcga cctgatgtac acgccgctca aacacttggg atacaaggcc
     1201 gtagtggtca atctctccga catctacgcc atgaacgccc gtcccaaaca gatcaccgtc
     1261 tccatcgcca tttccaaccg gttttcggtg gaggctttgg acgaactgta tgcgggcatc
     1321 cgggcagctt gcgagcgcta cggcgtggac ttggtgggcg gcgatacctc ctcctccaac
     1381 aaaggcctgc tcatcagcgt cacggccatc ggtcagggcg aagcggacaa gttgacctat
     1441 cgaaacaccg cccgcgtagg cgacctggtg tgcatcaccg gcaacctggg cgcagcgtat
     1501 ctgggtctgc aaatcttaga gcgggagaag cagcttttcc tgtctgatcc caaaatccag
     1561 cccaacctcg aagaccagag ctacctcatc ggacggcaac tcaagcccga agcccgcaaa
     1621 gagatgatcg agatgtttgc ccaacaacaa ctcgttccca cggccatgat ggacatctcc
     1681 gacggccttt cttccgacct catgcacatc tgccggcaat cgggcgtggg cgcgtacatc
     1741 gaagaaagcg gcgtgcccat tcaccccgaa gcgcagcaaa tggccatcaa attcggactg
     1801 gaccccatca cggtggcgct cagcggcggc gaagactacg aactgctctt caccattgat
     1861 cccaacgacg tggagaaggt gaaatatctg cctgacatct acatcgccgg cgagatagtg
     1921 cccgctgagg acggactgaa actgcatacc aaaggcggca atatccacgc catgacggcg
     1981 cagggctgga cgcattttgg gtgagttttg ttcttctact aatcattatc tccatcaaca
     2041 ttaattctgt caacatgaaa taccttattg ggttagcaat catcgggccg atcctcctcc
     2101 tctccgcctg caaatccgac aagcccgccg acaaagcgcc cgccgatgcg cctgccgccg
     2161 aagtgagcgc gccgcgtttc gaccgcgact ccgcctactt ttttgttgaa aaacaagtgt
     2221 cctttgggcc gcgcgtaccc aacacagccg cccataccgc ctgcaaagac tggtatgtgg
     2281 agcgcctccg ggcttacggc gcagaggtca tagagcaatc tttccggccc accgcctacg
     2341 acggcaccgt cttgcagtcc gtcaacatca ttgcaagata caaccccgcc gcgccccaac
     2401 gcatcgtgct ggccgcgcac tgggactcgc gccccttcgc cgattcgccc atcaataccg
     2461 agcgcaccaa agaccccgtt ctgggcgccg acgacggagc gagtggggta ggggtactgc
     2521 tcgaagtagc ccgccagttg caggcacaac ccctccaaaa catcggcatt gacctcgttt
     2581 ttttcgatgc ggaagactac ggcgatatgg gcggcaacaa cgataaatcc tggggcctgg
     2641 gagcgcaaca ttggtcgcgc aacctgcatg tgcccgccaa ccagttcaaa tacggcatcc
     2701 tcctcgatat ggtgggagcc aaaggcgcac ggtttgcccg cgagcaggta tccatgcgtt
     2761 tcgccccgca ggtgatggac aaagtgtgga aattggctca gggcctcggc tacggcaatt
     2821 attttgtgga tgaagacggc ggggctgtca ccgacgacca ctacttcgtc aacaacatcg
     2881 ccggcattcc catgattgac atcatcaacc gccccacgac gacccaaact ggttttggag
     2941 actactggca cacccacaac gacgacatgg acgtgataga cccgcgcacg ctgcgcgccg
     3001 tgggccaggt gttgctggaa ttgatttata aagaagacca atgattgcca tcgttcagta
     3061 caatgcaggc aatatccgct cggtgcaaaa tgcgctccag cgattgggtc ggcccagcat
     3121 tgtgacctat gtacccgaag aaatagaacg cgcctcgcat gtcatctttc cgggggtagg
     3181 ggaggctggt tcggccatga agtatctgag agacaaaggt ttggatgtgg tattgcaaaa
     3241 tctgaaacaa cccgttctgg gcgtgtgcct cggtttgcaa ctcctgtgcc gccatacgga
     3301 agaaggcgac acgcgctgcc tcggcatttt tgatgcggag gtgaaaagat ttcccccgca
     3361 ggataaagtg ccgcatatcg gctggaataa ctgcgcggcg ctgtcgggtc gtttgttgca
     3421 aagcattgat cctcagcagg atttctactt tgtacacagt tattatgccc aaacctgtga
     3481 acataccgcc gccgtttgcg attatatcct gcctttcagc gcggcgttgg aaaaggacaa
     3541 ctttttcgcg gtgcaattcc atcccgaaaa atcggccggc gccggtga
//
LOCUS       DAORLF010000463         3569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14252_length_3569_cov_1.729368, whole genome shotgun sequence.
ACCESSION   DAORLF010000463 DAORLF010000000
VERSION     DAORLF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_3569_cov_1.729368"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="PK198_24010"
     CDS             <1..1926
                     /locus_tag="PK198_24010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41888.1"
                     /translation="GGAGGRIIVPAGVMVQAGNLAGGQAGQSIQSTACAAGNNGAQPG
                     SAGTQGQVQLPLVESFTPVAPPMFAQQPLSMSICPGDSVVLTTSISGSISGLQWQLNT
                     GAGWVNLTNNATYSGAQNDTLIIRNITPAMSGYAYSLRASSLCYPDASSQPAVLTVLN
                     APAAMFTSNVSGSTVSFSNTSQAGSTFSWNFGDGQSSADSSPTHTYALPGTYTVQLIA
                     TNACGTDTATAAVLIIALPQAAFTANVTTGCTPLTVSYNSAGSGGVLTYAWSFPGGQP
                     ATSNQAAPTVTYQTAGTYDVRLIVGNASGFDTLLLPQYIQVSAAPTALFSAQPTGGLS
                     YNFSNSSLRADSYQWNFGDGATSTLTQPTHTYAMPGFYTVTLTTVNACGSAQYMVNIT
                     VGQMPSASFGQSVTEGCAPMAVQYNDQSTGVYTSRQWQFPGGTPATSTQQSQLVIYTL
                     PGTYSATLQLGGPLGMATFTQPNAVTAHPYPEPVFTFSVQALTVTFFNNSSNATSYSW
                     LFGDGNSSQAAAPVHTYAAPGTYTVTLNAQRPFCAASTTMTVMTGVTSSGEAAGAGAW
                     RVFPNPASERLFLQGPAEGRGCRYLFWNAAGQKVLEGTLRESSGELDLQDLPAGLYWL
                     ELVDENDLRQRLKVVVM"
     gene            2282..>2546
                     /locus_tag="PK198_24015"
     CDS             2282..>2546
                     /locus_tag="PK198_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41889.1"
                     /translation="MRKQLLPALRFALIAALSLSLFGCPTGLDFPLTEQPDKIDPTLI
                     GDWSANTLEAEILEVTVKKKDDYSYSIEVKETSEMYMLDDFKFV"
     assembly_gap    2547..2562
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2563..2794
                     /locus_tag="PK198_24020"
     CDS             <2563..2794
                     /locus_tag="PK198_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41890.1"
                     /translation="RLDGRTFLIAKPIESGEEKYYHYHYHFEGGMLVIQDMGLLVGGV
                     DAVTSSEALRKEVSASLKNPECLSSRMEYAKN"
     gene            2855..>3311
                     /locus_tag="PK198_24025"
     CDS             2855..>3311
                     /locus_tag="PK198_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41891.1"
                     /translation="MKYLFLILVFFVSAATATVLAQTPAETLEKAVTDYNALKTYVAG
                     LNPKTITDANVTDCKTRMDKGVAMLDQVIREGNSDEIKTARYFRVNFQYQYSFVLGMK
                     GENRQSLDVMRAIEREFTALKSSDFPLRYVFTGKNYIINWDNFAPTQAEY"
     assembly_gap    3312..3351
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3352..>3569
                     /locus_tag="PK198_24030"
     CDS             <3352..>3569
                     /locus_tag="PK198_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41892.1"
                     /translation="DAVRVSKIALAHPNSSDWLRYIAANKLLDINVKNNLLIPGAELE
                     ELALKSILLYDALPEENKKTVVEYNYPK"
BASE COUNT          824 a          950 c         1012 g          727 t
ORIGIN      
        1 ggcggcgcag gcgggcgcat catcgtgccg gcaggcgtca tggtacaggc aggcaacctt
       61 gcgggtggac aggcagggca gagcattcaa tctactgcct gcgcggcggg aaacaacggc
      121 gcacaacctg gctcggcggg cacacaagga caggtacaac tgcctttggt ggagagtttt
      181 acgccggtag cgccgcccat gttcgcacag cagccattga gcatgagcat ttgccccggc
      241 gattcagtgg tgttgacaac cagcatttcc ggcagcattt ccggcttgca atggcaactc
      301 aataccggtg cgggttgggt caatttgacc aataatgcca cctactccgg cgctcaaaac
      361 gacaccctga tcatcagaaa tatcaccccc gctatgtcgg gctatgccta ctctctgcgc
      421 gcctccagcc tgtgctaccc cgatgcaagc tcgcagcccg cggtgttgac ggtgctgaat
      481 gccccggcgg ccatgttcac atccaacgtg agcggcagca ctgtcagctt cagcaatacg
      541 agccaggcgg gcagcacttt tagctggaat ttcggcgacg gccaaagcag cgccgattcc
      601 tcgcccacgc atacttacgc cctgccagga acctataccg tgcagctcat tgcaaccaat
      661 gcctgcggga ccgatacagc gactgctgcg gtgctcatca ttgcattgcc gcaggctgct
      721 tttaccgcaa atgtgacgac cggctgcacg cctttgacgg tcagttacaa cagcgcggga
      781 tcgggcgggg tactgactta tgcctggtca tttccgggcg ggcaacctgc cacttccaat
      841 caggccgcgc ccacggtcac ttatcagacg gccggaacgt atgacgtgcg cttgattgtg
      901 ggcaacgcat ccggtttcga caccctgttg ctgccccagt acattcaggt atcggcagcg
      961 ccgacggccc tgttcagtgc gcagccgacg ggtggactca gttacaattt cagcaacagt
     1021 tcgctgaggg cagattccta tcagtggaat ttcggagacg gcgcaaccag cactttgacg
     1081 cagcccaccc atacttacgc catgccgggc ttttacaccg tgacgctcac aaccgtcaac
     1141 gcctgcggca gcgcacagta catggtgaac atcaccgtcg ggcagatgcc gtcggcgtct
     1201 ttcgggcaat cggtcacgga gggttgcgcg ccgatggccg tgcaatacaa cgatcagtcc
     1261 accggggttt acacctcgcg gcaatggcag tttccggggg gtacgccggc cacctctacg
     1321 cagcagtcac aattggttat ctacacatta ccagggactt acagcgccac gctgcaactc
     1381 ggcggcccat tggggatggc tacttttacg caacccaatg ccgtgacggc gcatccatac
     1441 ccggagccgg tctttacctt ttcagtacaa gctctgacgg ttacgttttt caataattcc
     1501 agcaatgcca cgagttattc ctggctcttc ggcgacggca actccagtca ggcggccgcg
     1561 ccggtacaca cttacgccgc gccgggaaca tataccgtga cgctgaacgc gcagcgcccc
     1621 ttttgtgcag ccagcactac gatgacggtg atgacaggcg tcacatcgag cggcgaggcg
     1681 gcgggggcgg gagcatggcg ggtgtttccc aatccggctt cggagcggct ctttttgcaa
     1741 ggtccggcag agggcagggg ctgccgctat ttgttttgga atgctgcggg gcaaaaagta
     1801 ctggagggaa cgctgaggga gtcgagtggc gaattggatt tgcaggacct gccggcgggt
     1861 ttgtattggc tggaattagt ggatgaaaac gacctgcgac agcggctgaa ggtggtggtg
     1921 atgtgagcaa agggccacaa caaatacgca attcatcgta ttgccgggca aagagaaagc
     1981 ctccatcttg cgctcaattt tagaaaatac caacacaaac caatcggatc attgtctgtc
     2041 gtgtacaacc gttccccgta cacgacggca gtggttcgca cctccgccgc ggcagggaca
     2101 gaggcggaat gaccgacctg aggagggcat ttgtagccga tggccccatg cctaccggca
     2161 ggcaggcggc gcccgcagcc cgctttctct cctcctttgg aggggccggg ggagcttggt
     2221 agagcgggcg gccgccccca aaacactgat ttttttttaa acagaaagcc aaaaatacga
     2281 catgagaaaa caattgcttc ctgcgttgcg ctttgcgttg atcgctgcgc tctccctgag
     2341 cctgttcggc tgccctaccg ggctggattt ccctctgacg gagcaacctg acaagataga
     2401 ccccacgctg atcggcgatt ggtcggccaa cacactggag gcagaaatac tggaagtaac
     2461 ggtgaagaaa aaagacgact attcttactc cattgaagtg aaggaaacct cggaaatgta
     2521 catgctggac gattttaaat tcgtagnnnn nnnnnnnnnn nngcggttgg acgggcgtac
     2581 attcctgata gccaagccaa tagaaagtgg ggaggaaaaa tattaccact atcattatca
     2641 ttttgagggt ggcatgctgg tcatacagga tatgggcctg ctggtgggcg gcgtagatgc
     2701 cgtgacgagt tcggaagccc tgcgcaagga ggtgagcgcg tcgttgaaga atccggagtg
     2761 cctgtcgagc cgaatggagt acgcgaaaaa ttaatgagag atactaatga tctgattgtc
     2821 aattaaaagc atcccgcgca aaagcataat gaccatgaaa tatttattcc ttatcctggt
     2881 attttttgtc agtgccgcta cggcaacggt cttggctcag acgccggcag agacgctgga
     2941 aaaggcggtg acggactaca atgccctgaa gacttatgtg gcggggctga accccaaaac
     3001 catcactgat gcgaatgtga ccgactgcaa aactcgcatg gacaaaggtg tggccatgct
     3061 ggatcaggtg attcgggagg gcaattcgga tgagatcaaa acggcccggt atttcagggt
     3121 gaattttcaa tatcagtact cgtttgtgct gggcatgaag ggcgaaaacc ggcagtcatt
     3181 ggatgtgatg cgcgccatag agcgggagtt tacggcgctg aaaagttcgg attttccgtt
     3241 gcgatatgtc ttcaccggaa agaattatat catcaactgg gataattttg cgcccacgca
     3301 ggccgaatat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagacgccgt
     3361 tcgggtatcg aaaatagcgc tggcacaccc caacagcagc gattggctgc ggtacatagc
     3421 tgcgaataaa ctgctggata tcaacgtaaa aaacaacctg ctgattccgg gtgcggaatt
     3481 ggaagaactg gcattaaaaa gcatcctgct gtacgacgca ctgccggaag agaataaaaa
     3541 gacagtggtc gaatacaact accccaagg
//
LOCUS       DAORLF010000464         3550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14349_length_3550_cov_2.008584, whole genome shotgun sequence.
ACCESSION   DAORLF010000464 DAORLF010000000
VERSION     DAORLF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14349_length_3550_cov_2.008584"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..718)
                     /gene="arsM"
                     /locus_tag="PK198_24035"
     CDS             complement(41..718)
                     /gene="arsM"
                     /locus_tag="PK198_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRF41893.1"
                     /translation="MTSSEEIKKIVRDKYSEIAMQGKAENASSCCGATNCGTYNIMSD
                     SYSDLEGYHPDADLGLGCGLPTEFAGIQQGDTVLDLGSGAGNDCFVARAEAGETGKVI
                     GVDFTPKMLELARQNAEKRGFNNVEFRQGDIEDLPVSADSVDVVVSNCVLNLVPDKAQ
                     VFSEILRVLRPGGHFSISDVVLRGHLPQPLVSAAEMYAGCVAGAMQMDEYLALIEKAG
                     FQNINGS"
     gene            complement(857..1183)
                     /locus_tag="PK198_24040"
     CDS             complement(857..1183)
                     /locus_tag="PK198_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF41894.1"
                     /translation="MGIHKKAAFAEDEQELAELAKALAHPARIAILKELTQRQTCICG
                     EIVEVLPLAQSTVSQHLKELLNAGLIQGTVDGVKSCYCINEEAFRKFERLFGQFFVHT
                     LKNSCC"
     gene            1351..>1656
                     /locus_tag="PK198_24045"
     CDS             1351..>1656
                     /locus_tag="PK198_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41895.1"
                     /translation="MLAAKEVFLPKSRHSASQLEARGSKLVAKKSKKTSTFMSSKSYI
                     HAFRIVSIAALVVLASWFILQKNSDVSTAASDHALSLKPKTEVQKMAEETVPAENLRA
                     "
     assembly_gap    1657..1666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1667..>2941
                     /locus_tag="PK198_24050"
     CDS             <1667..>2941
                     /locus_tag="PK198_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41896.1"
                     /translation="VATKVKTTTEPAAKPAKPAKYQRIGEAFEDRRLRTMDPALGYAP
                     SDRLLNVFSQAKRKQEAMLMDESKNSGDIGNARWRERGPSNIGGRTRSLLIDANDPSG
                     KLVWAGSVSGGLWRCNDVPSWTPINDYFPNLAVMDIVQHPSNPNLMYFCTGEWTDVRG
                     IGVFRSADGGNSWEHLPNTLGFQYSYKMLVHPNGDVYNTTSRGVWRSQDGGNNWTRII
                     GNGVSGVSSAVFYDIKLAADGNIYVSSNNTIYKSPSGNIGAWTSITNTAGAPPSSSQR
                     LEFDVSDSNPQVIFAVNNIGGDVEGIYKTINGGTSWAKMPLPNAFGMDNFARGQAWYD
                     LDIAIHPEDHNRIIVGGIDSHISTNGGIGWTQIGQWSGNGGIQYVHADQHRIVFDTKN
                     TNVAYLTNDGGVWRTQNVKAPGSQIVIRDRNN"
     assembly_gap    2942..2964
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2965..>3550
                     /locus_tag="PK198_24055"
     CDS             <2965..>3550
                     /locus_tag="PK198_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41897.1"
                     /translation="QFYAGAIHPETAKSYYIGGTQDNGSLALSDVGISAAREVLGGDG
                     FFCHIDEDEPNIQMVSLYYANYSLSTDGGRSFSGGAVLEGNFLSASDYDSRTNTLYSQ
                     TNAADFYRWNVSTGTTTPVDVDGLNLDVSAVTVDRNTPNRVYFGNFGNGRIVRVDNAH
                     TGETVSGVQLAPLTGTISGIAVEDGNAQHLMATVS"
BASE COUNT          818 a         1053 c          879 g          767 t
ORIGIN      
        1 ttttatttct cacaagccca cgccgcaaag cggcgattct tcacgaacca ttgatgtttt
       61 ggaagcccgc cttttcgatg agggccaggt attcgtccat ctgcatggcg ccggctacgc
      121 agcccgcgta catctcggca gcagaaacca agggctgtgg caaatggccg cgcagcacca
      181 cgtcgctgat gctgaaatgg ccgccgggcc gcagcacccg aaggatttcg ctgaaaacct
      241 gagctttgtc gggcaccaga ttgagcacgc aattgctcac caccacatcc acactgtcgg
      301 cgcttacggg caggtcttcg atgtcgccct ggcgaaattc cacattgttg aagccgcgct
      361 tttcggcgtt ttggcgagct aattccaaca ttttcggcgt aaagtccacg ccgatgacct
      421 tgcccgtttc gcctgcctcg gcgcgcgcca cgaaacagtc attgcctgcg ccggagccga
      481 ggtcaagcac cgtatcgccc tgttggatgc cggcaaactc ggtgggcagc ccgcagccca
      541 gccccagatc ggcatcgggg tggtagcctt ccagatcgga gtagctgtcg gacatgatgt
      601 tgtaagtgcc gcagttggta gcgccgcagc aggaagatgc gttttcggcc ttgccctgca
      661 tggcgatttc cgagtacttg tcccggacga tctttttgat ttcctctgat gatgtcatga
      721 tgtaaaggtg gttgattcta tcgtaaaaag acgatgattt tggtgaaaaa aaagtttcta
      781 tgcttagtga aggggtgaat cagtcaagtt cacaacctgt agtcacccct tcgctacctt
      841 gctgaatagc atgtactcag caacatgaat ttttcaaagt atgcacaaaa aactgcccaa
      901 acaggcgctc aaacttcctg aaagcctcct cgttgatgca atagcaagac ttgacgccat
      961 ccaccgtgcc ctgtatcaag ccggcgttga gcagttcctt caaatgctgg gaaaccgtgc
     1021 tttgggccag cggcagcacc tctactattt cgccgcaaat gcaggtttgc cgttgtgtca
     1081 gctctttcaa aatagcaatc cgtgcaggat gggccagtgc tttggccaat tctgccaact
     1141 cttgctcgtc ttctgcgaaa gctgcttttt tatggatgcc cattgataga aattaatcgt
     1201 aaaaaaacga tagaacagca tcgccgtgaa atgagttcaa agtgaaagga aagaacatac
     1261 accttaaaac ccctcttctg tcggccaaac tgccgatttc gttgttacca attaccaaaa
     1321 tgaggccgta tccgcaactt cgccttactt ttgctcgctg cgaaagaagt atttcttcca
     1381 aaatcgcgcc acagcgcttc gcaactcgaa gctcgcggct cgaagctcgt agctaaaaag
     1441 tcaaaaaaaa catccacatt catgtcttcg aaatcttaca tccacgcttt tcgcatcgtt
     1501 tccatcgccg cccttgttgt tctggcttct tggtttatcc ttcaaaaaaa ctccgatgtg
     1561 agtactgcgg catctgatca cgcgctatct ctaaagccta agaccgaagt gcagaaaatg
     1621 gcagaggaaa cggtacctgc ggaaaaccta cgggctnnnn nnnnnnccgt tgccacgaaa
     1681 gtcaaaacaa caactgagcc tgctgccaag cctgcaaaac ccgccaaata ccagcgcatc
     1741 ggcgaggcat ttgaagaccg ccgcctccgc accatggacc ccgcgctggg ctacgctccc
     1801 tccgaccgcc tgctgaatgt gttcagccag gccaaacgca agcaggaagc catgctcatg
     1861 gatgagtcta aaaacagcgg cgacatcggc aatgcccgtt ggcgcgaacg cggccccagc
     1921 aacatcggcg ggcgcacgcg ctccctgctc attgacgcca acgatcccag tggcaagctc
     1981 gtatgggccg gcagcgtttc gggtggtttg tggcgttgca acgacgtacc ctcctggacg
     2041 cccatcaacg actatttccc taatctcgcc gtgatggaca ttgtgcagca tccgtccaat
     2101 ccgaacctca tgtacttctg caccggcgag tggaccgacg tgcgcggcat tggcgttttc
     2161 cgctctgccg atgggggcaa ttcctgggag cacctgccca atacccttgg ttttcagtac
     2221 tcttacaaaa tgctcgtaca ccccaacggc gatgtttaca acaccaccag ccgcggcgta
     2281 tggcgcagtc aggatggcgg caacaactgg acccgcatca tcggcaacgg cgtgtcggga
     2341 gtaagctctg cggtgtttta cgacattaag ctcgccgccg acggcaacat ttacgtctcc
     2401 tccaacaaca ccatatataa gtcgccttcc ggcaatatcg gcgcctggac cagcatcacc
     2461 aataccgccg gcgcgccgcc atcctccagc cagcgtctgg agtttgacgt gtccgacagc
     2521 aatccgcagg tcatttttgc cgtcaacaac atcggcggcg atgtggaagg catttacaaa
     2581 accatcaacg gagggacttc ctgggccaaa atgcctctgc ccaatgcctt tggaatggat
     2641 aatttcgcac gcggccaggc ttggtatgac ctcgatattg ccatccaccc cgaagaccac
     2701 aaccgcatca tcgtgggcgg catagactcg catatttcca ccaacggcgg catcggttgg
     2761 acccaaatag gccagtggtc tggcaacggc ggcatccaat acgttcatgc cgaccaacac
     2821 cgcattgttt ttgataccaa aaacaccaat gtggcctacc tcaccaatga cggcggcgtg
     2881 tggcgcactc aaaatgtgaa agcgcccggc tcgcaaattg tcatcagaga ccgcaacaac
     2941 annnnnnnnn nnnnnnnnnn nnnnccagtt ctacgccggc gccattcacc ccgaaactgc
     3001 caaaagctac tacatcggcg gtacgcagga caacggctcg ctggcgttga gcgatgtcgg
     3061 tatttcagcg gcccgcgaag tactcggcgg cgatggcttc ttctgtcata ttgatgaaga
     3121 cgaacctaat attcagatgg tctcgctgta ctacgccaat tacagcctct ctaccgacgg
     3181 cgggcgtagc ttttcaggcg gggcggtgct cgaaggcaac ttcctcagcg cctccgatta
     3241 cgacagccgc acgaacacgc tctattccca aaccaatgcc gccgatttct accgctggaa
     3301 cgtttccaca ggtaccacca ccccggtgga tgtggacggt ctgaacctcg acgtctccgc
     3361 tgtgaccgtg gaccgcaaca cgcccaatcg cgtctatttc ggcaatttcg gcaacggacg
     3421 catcgtgcgt gttgacaatg cccataccgg cgagaccgtc agcggggtgc aattggcgcc
     3481 tttaacgggc accatctccg gcatcgcggt ggaagacggc aatgcacagc acctgatggc
     3541 tacggtttcc
//
LOCUS       DAORLF010000465         3533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14438_length_3533_cov_2.024439, whole genome shotgun sequence.
ACCESSION   DAORLF010000465 DAORLF010000000
VERSION     DAORLF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14438_length_3533_cov_2.024439"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PK198_24060"
     CDS             <1..852
                     /locus_tag="PK198_24060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41898.1"
                     /translation="WNRIPPEQLPTPLASEGFWITSGNGHYAAVGNKIWFGTRRGRIW
                     YSPDKGENWQVFALSSQNITNMSFSITFSDEQNGIAAGYGIAFRTNDGGQTWTPLTSL
                     PASIVYYRVQYVPGANGVCYLTYEGSQSFFNQYRHAYSLNNGDTWTLIADQPRLLPFE
                     FVSPTVGWGGGDIDGPDGKGMYRWSGNFGPASALQAPETAFSPRVFPNPFHNQMLLEF
                     DIEDNSLPLNITVSDLFGRTIQTFQFSRLHPGLNQLPLQVEAPPGMLLLTLRQGGRVQ
                     TVKVVRE"
     gene            1243..>3137
                     /locus_tag="PK198_24065"
     CDS             1243..>3137
                     /locus_tag="PK198_24065"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF41899.1"
                     /translation="MSTNFGLNSYDGQEFISFLAVPGDSTSLSSSNLSGCNPYEDPTG
                     SLWIATSLGGLNQYDYTTGKFRQYKADAGRPDWLASNQIVHITGDGTKGLWIATLGKG
                     LNYYDYESGAFRLYYAKEDSPENLMGGNTIPSLMQDSRGRLWIGTTRGASVFDGQDFT
                     RYRPDARQPRSLSSVFVSGLYEDRSGRVWIGTDNGLNLWNEEGKDFTRFFPSEYLHIN
                     GKGYDYIWEILQDSEGILWLGTNTGLLRFDPERRRFDPYLHNPSDPNSILPGPVHALM
                     EDHEKNLWIGTRQGISILNKASARFNRPEVQSLQASFGQITLARGIIACLEAQDNLWL
                     ATQKGLYTCRYGEAPQMILAGDFSALFYDDKGIIYAGTINQGFYRIFPDGKIIHTETF
                     ACLDSANIQIKPARINAFAEDRRGHIWVGILGGLWQFDASVSKCRKYFFSSTDQSSIS
                     SNEIRALLTDADGNLWITTNDGVNKLSTAELAKPWGSPLHFERFNHQRDNANSLSSNA
                     TFPILEDNKRNIWVGTDVGLNRYEPSIGKWTRFYTADGLPQDRITNIQEDRKGRLWIT
                     TYDGGVACFEPGSECFRNFFLEDGLNSRSYESFNVLVNHSGLILLAGTQGVNLFDPEE
                     VLRPPTA"
     assembly_gap    3138..3147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3148..>3533
                     /locus_tag="PK198_24070"
     CDS             <3148..>3533
                     /locus_tag="PK198_24070"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF41900.1"
                     /translation="RFTRFQIFNKDAPIGVEAGMLPQPIFMAPNIVLNHTHKVFSVEF
                     STFNFIHSGKKIYRYRLTNLGKDWIYIGKEPKITFANLRPGRYVLEVEFSDNQKDWIT
                     PEATLHFRILPPWYWAWWSKSLYLLL"
BASE COUNT          906 a          903 c          865 g          849 t
ORIGIN      
        1 tggaaccgga taccgcccga acaactcccg acacctttgg caagtgaggg tttctggatt
       61 acatcaggga acggccacta tgctgccgtc ggcaacaaaa tatggtttgg tacgcgcagg
      121 ggcaggatat ggtactcgcc cgacaaaggc gaaaactggc aggtattcgc cttgagcagc
      181 cagaatatta cgaacatgag tttcagtatt actttttctg atgaacaaaa cggcatcgcc
      241 gcaggatacg gcatcgcctt tcgcaccaac gacggagggc aaacctggac tccgctgaca
      301 tcacttcccg cttccatcgt ctattaccgc gtgcaatatg tgccgggcgc caacggggtc
      361 tgctacctga catacgaggg cagccaaagt ttttttaacc aatatcgcca tgcctattcc
      421 ctgaacaacg gagacacctg gacgctgatc gccgatcaac cgcgattgct ccccttcgag
      481 ttcgtttcgc ctaccgtcgg ttggggcggc ggagacattg acggccctga tgggaaaggc
      541 atgtaccgct ggagcggcaa cttcggccct gcttctgctc tgcaagcgcc ggaaacggca
      601 ttttcgcccc gcgtcttccc caaccctttc cacaaccaga tgcttctcga attcgatata
      661 gaagacaact ccctcccttt gaacatcaca gtcagcgacc ttttcggaag aacgatacag
      721 acctttcagt tttcccggct ccaccccggc ctgaaccaac tgccgctgca agtggaggcc
      781 cctccgggca tgctgctgct caccctgcgg cagggcggcc gggtgcagac ggtgaaagta
      841 gtgagggaat aatctgacgg ttttcaatgg cataccagga aggctcggcg ttggtgtcgg
      901 gcctttttta taccctacaa aaaaaagcgc ccttcaacga tagccgattt tgaaaaaaaa
      961 gcgggcagcc ccggataaaa aatgagtatt tttgtcgtcg aagcaatatc ctgatagaat
     1021 ctggaaatgt gaccgatgaa aagaccctgc tgtgtcaata gagcaaatgt aaaagcattc
     1081 ggacagtgcc tcatagccgc aatgacactg tgtatttcga catcgctttt gcacggacag
     1141 gcggacaccc ccgcttacaa attcattaac ctcggcacgg ctcaaaaatt atcaaatcta
     1201 tcggtaggcg gtttttacca ggacaaatac ggttttgtat gggtgtcaac aaatttcgga
     1261 ctgaacagct atgacgggca ggagttcatc tcctttctag cggttcccgg cgattccact
     1321 tcgctcagtt ccagtaattt gagcggctgt aatccgtacg aagaccccac agggagtctt
     1381 tggatagcca cctcattggg aggactgaac caatacgatt acacaacagg aaaatttcgg
     1441 caatacaaag cggatgccgg caggccagac tggctggcga gcaatcagat tgtacacatt
     1501 accggcgatg gcacaaaagg actatggatt gccacccttg gtaaagggtt gaattattat
     1561 gattatgaaa gtggggcttt ccggctttac tacgctaagg aagacagccc cgaaaatttg
     1621 atgggaggca acaccatacc ctcgctgatg caagacagcc gagggcgcct ttggataggt
     1681 actaccagag gagccagcgt ttttgatggg caggatttta cccggtatcg ccccgatgcc
     1741 cggcagcccc gcagcctgag cagcgtcttc gtgtcgggtt tatatgaaga tcgctccggc
     1801 agagtttgga tcggtacgga caatggcctc aatctctgga atgaggaagg gaaagatttt
     1861 acccgttttt tcccgagtga ataccttcat attaatggca agggttacga ttacatctgg
     1921 gaaattttgc aagacagcga agggatactc tggctcggca ccaacacagg tttgttgcgc
     1981 ttcgaccctg aaaggaggcg ctttgatcct tatctgcata atccttccga ccccaattcc
     2041 attttgccgg ggccggtgca tgcgctgatg gaggaccatg aaaaaaattt atggatcggt
     2101 acccgacagg gtatcagcat attgaataaa gcctcggcgc ggttcaatcg tccggaagtg
     2161 cagtcgcttc aagcttcttt cgggcaaatc accctcgcca ggggcattat cgcctgtctg
     2221 gaagcgcaag acaatctgtg gttggccact cagaaagggc tttatacctg ccgctatggc
     2281 gaagcgcctc aaatgattct ggcaggcgat ttttctgctt tgttttatga cgacaaaggg
     2341 attatatatg cgggaaccat caatcaaggc ttttaccgga tatttccgga tggaaaaatc
     2401 attcacactg aaacctttgc ctgtcttgat tcggcaaata ttcaaataaa acctgcacgc
     2461 atcaacgcat ttgcagaaga ccgtagggga catatctggg tgggaatatt gggggggcta
     2521 tggcagtttg atgcctctgt atctaaatgc cggaaatatt ttttttcatc cacagaccag
     2581 agcagcatct cttccaacga aatccgcgca ctgctcaccg atgctgacgg caacctttgg
     2641 atcacgacca acgacggcgt caataagctt tccaccgctg aattagccaa accttgggga
     2701 agccctctgc actttgaacg cttcaaccat caacgcgaca atgcaaattc attgagcagc
     2761 aatgcaacgt ttccgatatt ggaggacaac aaacggaata tttgggtcgg aacagatgta
     2821 ggactgaatc gctacgaacc ctccatcggt aaatggactc gattctacac tgccgatggt
     2881 ttgcctcaag atcgaattac caatattcag gaagatcgaa aaggccgtct ctggattacg
     2941 acttatgacg gcggagtagc ctgttttgag ccgggaagcg agtgttttcg caactttttt
     3001 ctggaggatg gcctaaactc acgaagctat gaaagtttca atgtattggt aaatcattcg
     3061 ggtcttatac tgctcgccgg aacccaaggc gtcaacctct tcgacccgga agaggtgctc
     3121 cggcccccta ctgccgannn nnnnnnnttc gcttcacccg cttccagatt tttaataaag
     3181 atgcacctat cggcgtcgag gcaggaatgc tcccacagcc gatttttatg gccccgaata
     3241 ttgtattgaa tcatacacat aaggtcttct ctgtcgagtt ttctactttt aattttatcc
     3301 attcaggaaa aaaaatctat cgctaccgcc tgaccaattt gggaaaagat tggatttaca
     3361 tcggtaaaga gccaaaaatc acctttgcca atctgcggcc cggcagatat gtacttgagg
     3421 tagaattctc tgacaatcag aaagactgga taacgccaga agctacatta cactttcgca
     3481 tcctcccgcc ctggtactgg gcctggtggt cgaagagtct ctacctgctc ctg
//
LOCUS       DAORLF010000466         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14446_length_3532_cov_2.160771, whole genome shotgun sequence.
ACCESSION   DAORLF010000466 DAORLF010000000
VERSION     DAORLF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14446_length_3532_cov_2.160771"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2118
                     /locus_tag="PK198_24075"
     CDS             <1..>2118
                     /locus_tag="PK198_24075"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41901.1"
                     /translation="NPQTFFWNLGNGTTSTDSLPPFPVYTTSDTVVSTYTITLISTNQ
                     CGGDTLSKEVVVYPPDVRAFIELDTLAGCQPLIIQAESFSTPGSIVSWLVIDPASNQE
                     GSILNAPQFTLRTPGRHTIVLYASRCGADADTAYVDVLPAPLVSFVHRPFICVGQPIA
                     FTNTSVNISGSEWTFGDGNNSTDYSPTHVFDEPGEYTVTLIAYSQANNCPATFSSTIS
                     VVGNPVAAFTPSATNGCGPLSIQFISNSTPGVNHVWDFGDGGAASYELNPTHTFQSPG
                     NYVVRLTVYDADSCFTDVAVSNIFVFPDPVASFSLPNQLNCLGHTSLQPTNLSEGAVA
                     YLWRWQNDTFNLIEPTIVPQTAGVFDLELIAVNTFGCRDTFVRSVDILSSPIASAIAL
                     VPAGCQPHSTGFSNNSQHASTFLWVFGDGNTSAFPTPSHRYLQPGVFQGLLIAGSADG
                     CPSDTAVFAVEVWPKPLADFDIDQAERCGAPSEVQFINRSDLSQSADWNFGDGSTSST
                     INPVHIYQTPGMYSATLVATNIYQCRDTTERIVDVYGRPRAAFATAGDTIGCEDFEVI
                     FRNTSTEALRYEWRIESFPNIFTEPAPQVVFTESGNYGVELIAIYNDLCRDTLRRSDY
                     IRVFRSPIAAFRFEVDNDPNILGDVIFINESQNADRFFWDFGDGTNSTLPNPEHEYNI
                     NRSIDVLLFAYNDNGGLFTCVDSI"
     assembly_gap    2119..2157
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2158..2464
                     /locus_tag="PK198_24080"
     CDS             <2158..2464
                     /locus_tag="PK198_24080"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF41902.1"
                     /translation="EWITTFFAPNALSPEFGPEGVGVFKPVGIGLQKYRIAVYSPWGE
                     QVWYSEVLENSRPVGSWNGRKHNEGDILPQGAYTWRADITFVNGVSKVVTGSVTLLR"
     gene            complement(2466..3449)
                     /locus_tag="PK198_24085"
     CDS             complement(2466..3449)
                     /locus_tag="PK198_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRF41903.1"
                     /translation="MPVPAPSTALLRHRALWNPDMYHGWGRHKRYFEGWYFKIVDPAE
                     QYAFAVIPGISYGADGSAHAFIQVLDGKRCTAAYHEFPAEQFVPSTQQFELQLGDNFF
                     SPQRIRLALPELSGELHFHHNFPWPRTLGAPGIMGWYAFMPFMECYHGVVSMHHRIEG
                     SLRVHDAQADFSGGIGYIEKDWGRSFPGAWIWTQTNHFEHTERVSLMASVADIPWLGS
                     RFTGYISGLLLEDRLFRFATYTGAKLHTELGEHQVMMSLSTRKLRLELIAHQAGGGEL
                     VSPIQGNMTGKVNESMQATVEVQLRENGKLLFEGTGRNAGLEVAGKVERLR"
BASE COUNT          768 a          948 c          913 g          864 t
ORIGIN      
        1 aatccgcaga ctttcttttg gaatctcggc aacggaacaa cttcgaccga cagcctgcct
       61 ccatttcctg tttataccac ctccgataca gtggtgtcca cttatacgat tacacttatt
      121 tccacgaatc aatgtggcgg cgatacgctt tccaaagaag tggtagtata tccgcccgat
      181 gtgcgcgcgt ttatagaatt ggacacactt gcgggctgcc aaccgctgat cattcaggcg
      241 gagagtttct ccacgcctgg gtccatcgtt tcctggttgg tcattgatcc tgcaagcaat
      301 caggagggca gcattctcaa tgccccgcaa tttacgctgc gaactccggg ccggcacacc
      361 attgtattgt atgcctcgcg ttgtggcgcc gatgcagata ccgcgtatgt ggatgtgctt
      421 ccggcgccgc ttgtgtcatt cgtgcatcgg ccttttattt gtgtggggca accgatagcg
      481 tttaccaaca cttccgtcaa tatcagtggc agcgagtgga cttttggaga tggtaacaac
      541 agcaccgatt attcacccac ccatgttttt gacgagccgg gggaatatac cgtaacgctt
      601 attgcatact cgcaggccaa taactgcccg gccacattca gcagtacgat ttcggttgta
      661 ggcaatccgg tagctgcatt taccccctcc gccacgaatg gctgtgggcc actgagtata
      721 cagtttatca gcaatagtac tcccggcgtc aatcatgtat gggattttgg cgatggcggg
      781 gccgcatcct acgaacttaa tcccacacat acgtttcagt cgcccggaaa ttatgtagtg
      841 cggcttactg tatatgatgc cgacagttgc tttaccgacg tggccgtaag caatatcttc
      901 gtctttcctg atccagttgc atcgttcagc ctgcccaacc aactgaactg cctggggcat
      961 acctccttgc agccgaccaa cctgtcggaa ggagccgttg catacctttg gcgctggcaa
     1021 aacgacacct tcaacctgat tgagccaacc attgtaccgc aaacagcggg cgtctttgat
     1081 ctggaactga ttgccgtcaa tacattcggt tgcagggaca cttttgtgcg cagtgtggat
     1141 atactctcgt cgcccatcgc atcagcaata gcccttgtac ctgccggatg tcaacctcat
     1201 tccaccggat tcagcaacaa ctcccaacat gcttctactt tcctctgggt atttggcgac
     1261 ggcaatacat ctgcttttcc gacgccctct caccgatatt tacagccggg cgtttttcag
     1321 ggtctgctca ttgccggcag cgccgacggg tgtccctccg atacggccgt atttgcggtg
     1381 gaagtgtggc caaagcccct cgctgatttt gacattgacc aggctgaacg ctgtggcgcg
     1441 ccttcggaag tgcagtttat caatcgctcc gacttgtcgc aaagcgcaga ttggaacttt
     1501 ggcgacggca gcacttcttc gaccatcaac cctgtgcata tttatcaaac gccgggcatg
     1561 tattcggcaa cgcttgtggc aaccaatatt taccaatgcc gcgatactac cgaacgcatt
     1621 gtagatgttt atgggcgccc gcgagcggcc ttcgccactg cgggggacac cataggctgt
     1681 gaggattttg aggtcatttt ccgaaatacg agtacggaag ccctgcgcta cgaatggcga
     1741 atagagtcct ttcccaatat ttttacagag cctgcccctc aagtagtttt tacagaatcg
     1801 ggtaattatg gcgtagagtt gatcgcgatt tacaatgacc tttgcagaga caccttgcgc
     1861 cggtccgatt acatccgggt cttccgttcg cccatagctg cttttcgttt cgaggttgac
     1921 aacgacccca atatattggg agatgtgatc tttatcaatg aatctcaaaa tgccgaccgc
     1981 ttcttctggg atttcggcga cggcacaaac agtacgctcc ccaacccgga acatgaatac
     2041 aatatcaacc gctccataga cgtattgctc tttgcataca acgataatgg cggtcttttt
     2101 acttgtgttg actccattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga
     2161 atggattacc accttttttg ctcccaatgc actgtcgccg gagttcgggc cggaaggggt
     2221 aggagtattc aaacccgtcg gcatcgggct gcaaaaatac cgtattgctg tttattcgcc
     2281 ttggggcgaa caagtatggt attccgaggt cttggaaaac agcaggccag tggggagttg
     2341 gaacggaaga aagcacaatg agggcgatat tttgccacaa ggcgcctaca cttggagagc
     2401 cgacattact ttcgtcaatg gtgtgagtaa agtcgtaacc ggaagcgtga ccttgctgcg
     2461 ttgaatcacc tcaaccgctc cacctttccc gccacctcca ggcccgcatt gcgccccgta
     2521 ccttcgaaga gcagcttccc gttttcgcgg agctgcactt ctacggtggc ctgcatactt
     2581 tcgttcacct tgcctgtcat attgccctgt atgggcgaga ccaactcccc gccgccggcc
     2641 tgatgcgcga tgagttccag gcggagtttt cgagtactga gagacatcat gacctgatgt
     2701 tcgcctaatt ctgtgtggag ttttgcacca gtgtaggtgg caaagcgaaa caagcggtct
     2761 tccaacagca ggccgctgat gtagccggtg aagcgactgc ccagccaggg aatatccgcc
     2821 acggaggcca tgagcgatac tcgttctgtg tgctcgaaat ggttggtttg cgtccatatc
     2881 caggcgcctg gaaaggatcg cccccagtct ttttctatat agccgatgcc gccgctgaag
     2941 tctgcctgcg cgtcatgcac ccgcagactg ccctctatgc ggtggtgcat actcaccacg
     3001 ccgtgatagc actccataaa cggcatgaag gcgtaccagc ccatgatgcc tggagcgccc
     3061 agcgtgcgcg gccagggaaa gttgtgatga aaatgcagtt cgccgctcag ttcgggcagc
     3121 gccagccgga tgcgctgcgg agagaagaag ttatctccga gttgcagctc gaattgttgg
     3181 gtggaaggca caaactgttc tgccggaaac tcgtgataag cagccgtgca gcgcttcccg
     3241 tcgagtacct gaatgaaagc gtgcgcgctg ccgtcggcgc cataagagat gcccggaata
     3301 accgcaaacg catattgctc ggcggggtca actattttga aataccatcc ttcgaagtag
     3361 cgtttgtggc ggccccagcc gtggtacatg tcggggttcc agagggcgcg gtggcggagc
     3421 agggcggtag agggagcggg tactggcatg atacaggatt ttttttgaat ggcgttgacg
     3481 taagtatttg ggtgaacaac accaatatgg cgggtctgtt gtcaaaaaaa ca
//
LOCUS       DAORLF010000467         3531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14449_length_3531_cov_1.777330, whole genome shotgun sequence.
ACCESSION   DAORLF010000467 DAORLF010000000
VERSION     DAORLF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14449_length_3531_cov_1.777330"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1210
                     /locus_tag="PK198_24090"
     CDS             110..1210
                     /locus_tag="PK198_24090"
                     /inference="COORDINATES: protein motif:HMM:NF027648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4960 domain-containing protein"
                     /protein_id="HRF41904.1"
                     /translation="MKQLVQISTFLLLFISAWSCSKRENYIEGPSILDIIEEVKINNK
                     PASIDHLSGLIQVSLPGNTDLRNVTYVATAPAGVTISPAGGTPLNLEQPVTVTADNGF
                     SRRIYRIVATLLPSKIACLGDGATIAQISDDDVQEAAEWAQQTYGDDFVYIPYAELDD
                     EALLGVNVIFYMHDQVGSSAQPAALLEKLNVLSKFYVQGGKILAGQHGTGIVEELGRD
                     RSGLRTIIGTGAGGPNADTWAVGFTSSPIANILTNGVAFNANGQVNVIDGGFKEDHNS
                     MWTMDPLAAPKYGSFSTLYNAEVLATWDWNVASQGTGGIILWNPSGRFRGTIITLGVG
                     GMEWRMNDGRPNAYAQNIRTIYKNAIDYLKGL"
     gene            1287..3422
                     /locus_tag="PK198_24095"
     CDS             1287..3422
                     /locus_tag="PK198_24095"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HRF41905.1"
                     /translation="MRTTLIFLAALGLAWHVCKAQQQPIYQCRFEPGWTNPVSGHVWQ
                     GRSEQPELAEGLIGRALRLDGYSTFVHSKLKRAITAPLTVSAWVALETYPTHPAGCFS
                     LVSDSDPYITAGITNFGQPYIRTSGGRVVADVMVPKFRWQHLALIVDADSAKLVWNGQ
                     IVAATANGRAAAYTHIYLGRDAAAGFVHIFPTMHINGLLDEVTIWEGAKQPTELPKWI
                     SMEKTGLDADLCIPAGRFAHDHTRPQYHLLPLANWTNETHGLLYYNERYHIFNQKNGA
                     NVFLGNINWGHFSSPDLIHWTEHRPVLSPGPGYDQMGIWSGHVVPDEEGSPVIFYTGS
                     DGTVFSICRASPQDSSLLSWGKSKANPLIKGNPPQYHRADLRDPYVWKEDSAWYMVVG
                     YGILEDSTEQGALLLYRSQDLTAWEYRGIFFHHKPWPETPGIFWEMPVFWKMGDKYIL
                     LVNPVPYQKKPARAIYWVGEFKQERFVPDHEQPRHLEIINRLLSPSVAYDEEGRTTAI
                     AIIPDLIPAEMQYQRGWTHLYSIPRLWELKDSVLLQSPHPALKKLRGTPLQRSKLHLQ
                     EGDTLAIAKGQQLELLLKLDISRTSSFNLVVGKNAHNGEATSLHFDLEAAALLIDHNK
                     SSLNPLFEKRKETGIFHHRPQEPLLLHIFVDGSVLEGFVNNKEAFTTRFFTTDPVSDE
                     IELVIQKGSLQVLDMTVWPITPAKVSCGF"
BASE COUNT          877 a          918 c          903 g          833 t
ORIGIN      
        1 cggctacgaa tttgactcga acggcaccgt gaaccagtat caaaataatc tggaaacatt
       61 cacgcgcaat atcattgact atttgcgaac cttataaaac atggataaga tgaagcagtt
      121 agttcaaatc agcacattcc ttttgctgtt catcagcgcg tggtcgtgct cgaaacgcga
      181 aaactacatt gaagggcctt cgatactcga catcatcgag gaggtgaaaa tcaacaacaa
      241 accggccagt attgatcacc tttcaggtct gattcaggtc agcttgcccg gcaataccga
      301 cctccgcaac gttacttatg tcgctacggc cccggctgga gtgacgataa gcccggccgg
      361 cggtactccg ctcaatctgg agcagcccgt aaccgtcacc gccgacaacg gtttcagccg
      421 gcgtatttac cgcatcgtag ccactttgct gccctcgaaa attgcctgtc tgggtgatgg
      481 ggccaccata gcccagattt cagatgacga cgttcaggaa gccgctgagt gggcccaaca
      541 aacttacggt gatgatttcg tttatatccc gtatgccgaa ctcgacgatg aggcgcttct
      601 gggagtcaat gttatctttt acatgcacga tcaagtgggg tcttcggcac aaccggccgc
      661 tctgttggaa aaactcaacg tattgtccaa gttttatgtg caaggcggca agatactggc
      721 cggccagcat ggtacgggga ttgttgaaga gctcggccgc gatcggtccg gcttgcgaac
      781 gatcatcggc acgggggcag gcgggccaaa tgccgatacc tgggccgtcg gctttacgag
      841 ttcacccatc gccaatatcc ttacaaacgg tgtagctttc aacgccaacg ggcaggtcaa
      901 tgtcattgac ggaggcttca aagaagacca taattccatg tggacgatgg acccgctggc
      961 cgcgcccaag tatggcagtt tctccacgct ttacaatgcg gaagtgttgg ctacgtggga
     1021 ctggaacgta gcttctcagg gaacgggcgg tatcatcctt tggaatccaa gcgggcgttt
     1081 ccggggtacc attattacgc ttggcgtcgg cggtatggag tggcgtatga acgacgggcg
     1141 ccccaatgcc tatgctcaaa atatccgtac catctataaa aatgccattg actacctgaa
     1201 aggattgtaa tctgatagaa gtagaaggga tgcctgcgtc tgtctgggac ggggcatcct
     1261 cttttctttt ttgataattt cttaccatgc gaactaccct gatttttctt gccgccctag
     1321 ggctggcatg gcatgtttgt aaggctcagc agcagccgat ttaccaatgc cgctttgaac
     1381 cgggctggac caatcctgta tcgggccacg tatggcaggg aagatccgaa cagcctgaat
     1441 tggcggaggg gctgatcggc cgggcactaa gactggacgg ctactctact ttcgtgcatt
     1501 ctaagctgaa acgcgccata acggcgccct taacggtaag cgcatgggtg gctctggaaa
     1561 cctatccgac gcaccccgcg ggatgttttt cactggtaag cgactctgat ccatacataa
     1621 cagcgggtat caccaatttt gggcagccat acattagaac ctcgggtggg cgcgtcgtag
     1681 ccgatgtgat ggtgccgaaa ttccgctggc aacatctggc gttgatcgtt gatgcagaca
     1741 gcgcgaaatt agtatggaac gggcagatcg ttgcggcaac agcaaatgga cgagcagccg
     1801 cttatacaca tatttatctg gggcgggatg ctgctgccgg ctttgttcac atttttccaa
     1861 ccatgcatat caatggcctc ctggatgaag tgaccatctg ggaaggcgcc aagcagccaa
     1921 ccgaattgcc gaagtggata tctatggaaa aaacaggcct ggatgcagat ttgtgtattc
     1981 ccgccgggcg ttttgcccac gatcacaccc ggccccaata ccacctcttg ccgcttgcca
     2041 attggaccaa tgaaacgcat ggcttgctat actacaatga gcggtaccac attttcaacc
     2101 agaaaaacgg cgccaacgta tttctcggaa acatcaactg ggggcatttc agcagcccgg
     2161 atctgataca ttggacggag catcgccccg tgttatcccc cggccccggc tacgatcaga
     2221 tgggaatatg gtcagggcat gtagtgcctg acgaggaagg ctcaccggtt atcttttata
     2281 ccggcagtga tggaacggtt ttcagcattt gccgtgcatc cccacaagac agtagtttgc
     2341 tatcctgggg aaaaagcaaa gccaaccctt tgatcaaagg caatccgcct caatatcacc
     2401 gggccgacct gcgcgatccc tatgtctgga aagaggatag cgcatggtat atggtggttg
     2461 gttatggtat tctggaggac agcacagaac aaggcgcatt attgctctat cgttctcagg
     2521 atttaaccgc atgggaatac agggggatat ttttccatca caagccctgg cccgaaactc
     2581 ccggtatatt ttgggaaatg ccggtatttt ggaaaatggg cgataagtac attttgttgg
     2641 tcaatcctgt tccctatcaa aaaaagcccg caagagccat ttactgggtc ggcgagttca
     2701 aacaagagcg ctttgtaccc gaccacgagc agccccggca tcttgaaatc atcaaccgcc
     2761 tgctctcccc gtctgtggca tacgatgagg aggggagaac tactgccata gccatcattc
     2821 cggatttgat ccccgccgaa atgcagtatc aacggggatg gacgcattta tacagcatac
     2881 cccgcttatg ggaactgaaa gactccgtgt tgctgcaatc tccgcatccc gcattgaaaa
     2941 aactacgcgg cacgccattg cagcgatcca aactccacct ccaggagggc gatacacttg
     3001 ccatagccaa aggacagcaa ttagagctgc tgctgaagct ggacatcagc cggacgagtt
     3061 cattcaacct cgttgtcggg aaaaacgcac acaatgggga agccacttcc ttgcactttg
     3121 atctggaggc ggctgcttta ctcatagacc acaacaagag cagtctgaac cccctgtttg
     3181 aaaaacgaaa agaaacaggt atttttcacc accgcccaca agagccgctt ttgctgcaca
     3241 tctttgtgga tggttccgtt ctggagggct ttgtgaataa taaagaagcg tttactaccc
     3301 gtttttttac aaccgatcct gtaagcgatg aaatcgaact cgttatacaa aagggtagct
     3361 tgcaggtgct ggacatgacc gtttggccaa tcactccggc gaaggtttct tgtggatttt
     3421 gataccctgt aaaaaccaac cacatgcgga tatacctttc catcgtacta ctcgtagcag
     3481 tttctgcagc cgcagcgcag gcccctattt ttcatctcga ttttaatgaa g
//
LOCUS       DAORLF010000468         3527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14476_length_3527_cov_1.643145, whole genome shotgun sequence.
ACCESSION   DAORLF010000468 DAORLF010000000
VERSION     DAORLF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14476_length_3527_cov_1.643145"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..924)
                     /locus_tag="PK198_24100"
     CDS             complement(208..924)
                     /locus_tag="PK198_24100"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF41906.1"
                     /translation="MLKKAYSTTQSVCKVTFSLPVEAAPDAEKVVLVGDFNNWNTQEA
                     PVLKKSKNQFETSLELAAGRSYEFRYLIDNKRWENDWEADDYTPSPFTGITNSVLVLD
                     AVEVTPAAPAKKAAAPKKASTAQPVAAEVPAEKPAPKAKAVKVAKPAAPASVVKDDLT
                     KIEGIGPKIAEIFATKGIATFADLSKAKPATLKTILDEAGSRFKMHDPTTWPAQAKLA
                     AAGSWEKLQTMQDELKGGKK"
     assembly_gap    1256..1314
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1359..>1921)
                     /locus_tag="PK198_24105"
     CDS             complement(1359..>1921)
                     /locus_tag="PK198_24105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41907.1"
                     /translation="DPFQVMLYYNEYQTVVILDRTLNLLSSINLWEFGIRQPLAPAMS
                     NDNMLWVYDHADFRLRKLDRHGNPLHDSGDLNSLLNATPRPAEITARHNLVVLNDPNI
                     GLLLFDNFGQYLETLPFPGCSQVQLPEPDRILFYCNRRWKSYHIELRREVAFNLPSDM
                     SPEAQIVAVGNYIFALHGDKIHCWSR"
     assembly_gap    1922..2077
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2078..2298
                     /locus_tag="PK198_24110"
     CDS             <2078..2298
                     /locus_tag="PK198_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41908.1"
                     /translation="PFKIQGERMLTEYCLREEQNRKAKKKGMDRNETQLHIELTLNRG
                     ICGAFAGAFLSRCGNSWCSRTGQGCVAY"
     assembly_gap    2308..2336
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2337..>3527)
                     /locus_tag="PK198_24115"
     CDS             complement(<2337..>3527)
                     /locus_tag="PK198_24115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRF41909.1"
                     /translation="NRLVQLIVDETNREFPGKNYKADDLLAYQMPKELPIVSIRIFSK
                     NALKALRAMPEVRYVEPMGYSPEEVNFRSDAGCGVNPASSIPSADFQTISPSVKVPWN
                     FNLHNIQQAWAQSTGAGIWVALIDTGTSPNQTKLQQNGEFNSGQSSGRTITRLGTYVS
                     SWWPWASPDGPNDQCGHGTQMAGLIAAPRGSGGASVGVAYNSNLLAIRATGDVIVNGS
                     SEITGVANAITIAANNTNVKILSMSIGDVFWSNTVADAIYYAYYSKGKMLISAAGTSL
                     SWTSWWGVIFPANMAETVAVTGVKEGYPLVRCDVCHSGSEVDFIIMMQRRNSSNTSLT
                     LAMSGNTPSYVGGSSTATAMTAGIAALVWAKNPSQTRTQVLNKLKNAASIYPSRNSEF
                     GWGLI"
BASE COUNT          748 a          734 c          944 g          857 t
ORIGIN      
        1 ctttaaaaat gccgtctttt tgttttttca tttgaatgcc gtcttcggca ttccagccgt
       61 tgaattcgcc caggagcact accgttttag cttcgggagc caattctgcc gggatggaaa
      121 aagtggcctt gcaaacgggc ttggacttca tgaactgctt ggtaatcatt gtcttaaagg
      181 ttatatggtg aaaaaatcgg aatgcggtta ctttttgccc cctttcaatt cgtcctgcat
      241 ggtttggagc ttttcccatg aaccggctgc ggccaatttg gcctgagcgg gccaggttgt
      301 gggatcgtgc attttgaacc ggctgcctgc ttcatcaagg atggtcttca gtgtggcagg
      361 tttggcttta gacaagtctg cgaaagtggc aatacctttt gtagcaaaaa tttcagcgat
      421 tttaggaccg atgccttcaa ttttggtcaa atcatctttg accacagacg ccggggcagc
      481 aggtttggct actttcacag ctttggcctt gggcgccggt ttttctgcgg gcacttcggc
      541 tgcaacaggt tgggcggtgc ttgctttttt aggagcagcg gcttttttag ccggagcagc
      601 cggtgttact tcaacagcat ccagcaccaa cacggagttg gtaatgccgg tgaaaggcga
      661 cggagtgtaa tcatcggcct cccagtcgtt ttcccagcgc ttgttgtcaa tcaggtacct
      721 gaactcgtaa ctacgtccgg cagccagttc taaggatgtt tcaaactggt ttttactttt
      781 tttcaaaacc ggcgcttcct gagtgttcca gttgttgaaa tcgccgacca acacaacttt
      841 ctccgcatcc ggtgcagctt cgaccgggag agaaaatgtg actttgcaaa cggattgagt
      901 ggtagaatat gctttcttca acatgatgtt gcgtgattgt gagtgagata atgtttggtc
      961 aagcgccttg attaaaaatt aaattttttt agtgtttttg aaacactaag cggattgacg
     1021 ctgcaaagtt acgacttatt gcactgattt tcaatatctg ttgaaaaaaa aatacaccct
     1081 ggatagaagt caagactttt tggcgagata ccggccggaa attttgcacc taaattaaag
     1141 aatatgtatg aaaataatta tcaattttcg gaataaaatt tcaaaaaaca aaatacttgg
     1201 gaaaaaccaa ttttcaggaa tgaggtcgaa aaaaaagtac acattttcgg ggaacnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgacg
     1321 gttccctaaa agcgtgtggg atattcggac cccgttcctt acctgctcca gcagtggatt
     1381 ttatccccgt gcagtgcaaa aatgtaattt ccgactgcga cgatctgagc ttcgggtgac
     1441 atatcggatg gcaaattgaa ggccacttcc ctgcgaagtt caatatggta actcttccac
     1501 cggcgattgc agtaaaacag tatccggtcg ggttcgggca attggacctg cgagcagccg
     1561 ggaaagggca gcgtttcgag gtattgcccg aagttgtcga acaagagcag acctatgttg
     1621 gggtcgttga gtacaacgag gttatgcctg gccgttatct cagcagggcg tggcgtggcg
     1681 ttgagcaggg agttgaggtc gccgctgtcg tgcagcgggt tcccgtggcg atctaatttt
     1741 cggagcctga agtcggcatg gtcatatacc caaagcatgt tgtcgttgct catggccgga
     1801 gcgaggggtt gccggatgcc gaattcccat aaattaatgg atgataagag gttgagtgtt
     1861 ctgtctaaga ttacaactgt ttgatattca ttataataca gcattacctg aaaaggatcg
     1921 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc cattcaaaat tcaaggtgaa
     2101 agaatgctga ccgaatactg cctgcgagag gagcagaacc gaaaggcaaa aaagaaaggc
     2161 atggatagaa acgagacgca attgcatatt gagctaacgc ttaaccgggg gatttgtggc
     2221 gcttttgccg gggcattttt gagccggtgc gggaattcat ggtgttcccg caccggacaa
     2281 ggttgtgtag cttattgact tacagccnnn nnnnnnnnnn nnnnnnnnnn nnnnnngata
     2341 agcccccatc cgaactcgct gttgcgcgag gggtaaatgc tggcggcgtt tttgagcttg
     2401 ttgagtacct gtgtgcgggt ttgagacggg tttttcgccc aaaccaatgc cgcgatacct
     2461 gccgtcatgg ctgtggcggt ggaggagcct cccacatacg agggagtatt gccactcatg
     2521 gccaatgtga ggctggtgtt gctgctgttg cggcgttgca tcatgatgat gaagtccact
     2581 tcgctgccgg aatggcaaac atcgcagcgc acaagcggat aaccctcttt cacacctgta
     2641 actgctacgg tttcagccat gttggccgga aagataacgc cccaccaact ggtccagctc
     2701 agagaagtgc cggcagccga gatgagcatt ttgcccttgc tgtagtaggc gtagtatatg
     2761 gcgtcggcga cggtgttgct ccagaagacg tcgccgatgg acatgctcag gattttgaca
     2821 ttcgtattgt tggcggcgat ggtgatggca ttggcaaccc cggtaatctc gctggaaccg
     2881 ttgacgatta cgtcgccggt agcgcgaatg gccaacaggt tggaattgta ggccacgcct
     2941 acggaagcgc cgcccgaacc gcgcggagcg gcaatgaggc cggccatttg ggtgccgtgg
     3001 ccgcattggt cgttggggcc gtcgggggag gcccagggcc accaggaact gacataggtg
     3061 ccgaggcggg tgatggtgcg tccggaagac tgaccgctgt tgaattcgcc gttttgttgc
     3121 aatttcgttt ggttcggaga ggtgccggta tcaatcagcg ctacccagat gcctgcgccg
     3181 gtagattgcg cccaggcctg ttggatgttg tggagattga aattccaggg cactttgacg
     3241 gagggagaga tggtttggaa gtcggcggag gggatgctgg aggcagggtt gacgccgcaa
     3301 ccggcatcgc tgcggaagtt gacttcttcg ggcgagtatc ccatcggctc cacatagcgc
     3361 acttcgggca tggcgcgcaa ggctttgagt gcatttttgc tgaaaattcg gatgctgacg
     3421 atgggcaatt ctttcggcat ctggtaggcc agcaggtcgt cggctttgta gtttttaccg
     3481 gggaactccc ggttggtttc atccacgatg agttggacga ggcggtt
//
LOCUS       DAORLF010000469         3523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14498_length_3523_cov_1.581315, whole genome shotgun sequence.
ACCESSION   DAORLF010000469 DAORLF010000000
VERSION     DAORLF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14498_length_3523_cov_1.581315"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>208)
                     /locus_tag="PK198_24120"
     CDS             complement(<1..>208)
                     /locus_tag="PK198_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41910.1"
                     /translation="NQNGSPLPGMIRNYIADRMLKLPHRDWHGQAKTAREKAKTATAQ
                     QPQPDSLMRKTGTLPSHALADYAGM"
     assembly_gap    209..450
                     /estimated_length=242
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<451..1289)
                     /locus_tag="PK198_24125"
     CDS             complement(<451..1289)
                     /locus_tag="PK198_24125"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF41911.1"
                     /translation="MRHLILFLLVLLSFSLTAQRGGKKVQPIQADQRLTGLDTLVNRL
                     LREWHAPGVGIAVVEKDKVLFAGGYGFKDYEQKQPVSGNTLFAIGSCSKAFTSSLLGL
                     LQSEGKLDFDKPVTEYLPGLKFYTPEMGNNITVRDLTCHRTGLPRHDFSWYLNPASRD
                     SLVQRIQHMEPSATWRQAWQYNNWMFLLQGVIAEKLYEKTWEELISEKIFAPLGMRSS
                     NFAPWDGNYPDLAKGYRTDDQDMIQHMDYYRIGGMGPAGAIYSNPTEMAAWVTAWIYG
                     GEY"
     gene            complement(1469..1828)
                     /locus_tag="PK198_24130"
     CDS             complement(1469..1828)
                     /locus_tag="PK198_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRF41912.1"
                     /translation="MNSTHDERIARMIFASVYPHYVSKVEKKGRTKEELDQVIEWLTG
                     FDPQQLQEQIDQKATFEAFFEHATLHPNAHLITGLICGYRVEEIENPLTQKARYLDKL
                     VDELAKGKKMEKILRER"
     gene            1949..2533
                     /locus_tag="PK198_24135"
     CDS             1949..2533
                     /locus_tag="PK198_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41913.1"
                     /translation="MHYIKLKSEGTVIEFHNNWLGEETVIVGGKKVSKKSSIMGANHY
                     FTVIENGAPVNYVLTSKVLDLTMMNVGVDLIRNGQIVYANVPLSYGTKPKSPHEELKS
                     KALAKLIAYEIHEALDLLKHAQQLDPEDYEIYFHLACAYSNLEDVENGYANLKLAVQH
                     GMSDHEKILNHDMLAYLRLQDPFERFQESGFTEY"
     gene            complement(2660..3001)
                     /locus_tag="PK198_24140"
     CDS             complement(2660..3001)
                     /locus_tag="PK198_24140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRF41914.1"
                     /translation="MSETIKPCPNCMSPYGYAAGEDLFACPECGHEWNPENLADADVL
                     KVLDANGNELKTGDSVIVIKDLPVKGAPKPVKAGTKVKNIRLTDGDHNIDCKIEGFGA
                     MALKSEFVRKA"
     assembly_gap    3090..3182
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3256..>3523)
                     /locus_tag="PK198_24145"
     CDS             complement(3256..>3523)
                     /locus_tag="PK198_24145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41915.1"
                     /translation="ENWLENAGVRIFPNPGWDLVQVEMQLDAPATVHWQLTDVNGRAV
                     AGGIWEAPEGTLRRSIEAGHLPQGVYFVRLQTQEGFGVKKWVKR"
BASE COUNT          726 a          792 c          813 g          857 t
ORIGIN      
        1 acataccggc ataatcagcc agtgcatgag agggcaaagt acccgttttg cgcatcagac
       61 tgtcgggttg tggttgttgc gcagtggcag ttttggcttt ttcccgggcg gttttggctt
      121 ggccgtgcca atcgcggtgc gggagtttca acatgcggtc tgcgatgtaa ttgcgaatca
      181 tgccgggtag cggcgagccg ttttgattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgtattccc cgccgtaaat ccaggccgtt
      481 acccatgccg ccatttcggt tggattggaa taaatggccc ctgccggccc catgccgcct
      541 atgcggtagt aatccatgtg ctgaatcatg tcttgatcgt cggtgcggta gcctttggca
      601 aggtcgggat agttgccgtc ccagggtgcg aagttggaac tgcgcatgcc cagcggagcg
      661 aatatttttt cggagatcag ttcctcccag gttttttcgt agagtttttc ggcgatcacc
      721 ccttgcagca ggaacatcca gttgttgtac tgccaggcct gcctccaggt tgcggagggt
      781 tccatgtgct ggatgcgctg cacgaggctg tcgcggctgg cggggttgag ataccaggaa
      841 aaatcgtggc ggggcagtcc ggtgcggtgg caggtcaggt cgcgcacggt gatgttgttg
      901 cccatttccg gggtgtaaaa cttcagtccc ggtaggtact ccgttaccgg cttgtcaaaa
      961 tcgagtttgc cctcgctttg cagcagcccc agcaatgaac tggtaaacgc cttggagcag
     1021 gagccgatgg cgaagagcgt gttgccgctc accggttgtt tttgttcgta atctttaaac
     1081 ccgtacccac cggcaaaaag gactttgtct ttttccacta ctgcaatgcc gactcccggc
     1141 gcatgccact cgcggagcag cctgttgacc aaggtatcta agcccgtaag gcgctgatcg
     1201 gcctgtatgg gttgtacttt tttgccgccc cgctgggccg tcagagaaaa actgagcaat
     1261 accaagagga agagaatcaa gtggcgcata atgtggtgcg tttggtgttg cccaaaggta
     1321 gggaaagtct gggtttaagg ttttgatttc catattttat cggaaaaagg caaccaataa
     1381 tgccaatgtc agcatacatt tcattgtctt tctgtttttt gttgtgatgt ttttcccata
     1441 ttggggatag gtccgctcgt gccggccctt acctttccct caaaatcttc tccatttttt
     1501 tgcctttggc caactcgtcc actaatttgt ccaaatacct ggctttttgg gtcagcgggt
     1561 tctcgatttc ctccacgcga taaccgcaga ttaggcccgt aataagatgc gcattggggt
     1621 gcagtgtggc atgttcaaag aaagcttcaa acgttgcctt ttggtctatc tgctcttgca
     1681 gttgttgcgg gtcgaagccg gtcagccatt caatgacctg atccaattct tcttttgtgc
     1741 ggcccttttt ctccactttg ctgacgtaat gagggtaaac ggaggcgaaa atcatgcggg
     1801 caatgcgctc atcgtgggtg ctgttcatgg tgtgcgtttt ggtatctgtt tggcaaagat
     1861 agcaaattca tcttaccgcg ccaaccaaaa taaaggcggg gtgttctgac ggaattcgcg
     1921 agtacaaaac caacacaatt cagcaatcat gcactacatc aaactcaaat cggaaggcac
     1981 cgtcatagaa tttcacaaca actggctggg agaggaaacc gtcattgtgg gcggcaaaaa
     2041 ggtatccaag aaaagctcca tcatgggcgc caatcattat ttcaccgtca tagagaacgg
     2101 ggcgcccgtc aattatgtgc tgacttccaa agtgttggat ttaaccatga tgaatgtcgg
     2161 cgttgatttg ataagaaacg gccagatagt ttacgccaat gttccgttga gttatggaac
     2221 caagcccaaa tcaccgcatg aagaactcaa atcaaaagca ttggcgaaac tcatagctta
     2281 cgaaatccat gaagcattgg atttgctgaa acatgcgcag caattagacc ccgaagacta
     2341 tgaaatttac ttccatctgg cttgtgcgta ctccaacctc gaagatgttg aaaacggcta
     2401 cgccaacctg aaattggccg tgcaacatgg catgagcgat cacgagaaaa tcctcaacca
     2461 cgatatgctg gcctacctca gattgcagga cccgtttgag cgctttcagg agagcgggtt
     2521 tacggagtat tgatggggaa gtgagatttt gaaaattgag gtgaggatat gatgtgggtt
     2581 agatttgaca aattttcaaa atttgtcaaa tctttcatcc cataatcaca ccttcactaa
     2641 gcatggaatc tcaactaaat tatgccttcc tcacaaattc agatttcagc gccatagcgc
     2701 cgaacccctc aattttgcag tcaatattgt ggtcgccgtc cgtcagccgg atgttcttaa
     2761 ctttcgttcc cgctttgacg ggcttgggcg cacccttcac cggcaagtct ttgatgacaa
     2821 tcaccgagtc tccggttttc aattcgttgc cgttggcgtc caacaccttc aatacatcgg
     2881 cgtcggcgag gttctccggg ttccattcgt gcccacattc aggacaggca aaaaggtcct
     2941 ctccggcggc ataaccatag ggcgacatgc agttagggca tggctttatt gtttcagaca
     3001 ttctgatatt tgtttttgca tctgtttggt tggatgtttt caggtgacat ctaacagccg
     3061 agcttgagcg aggcgggaag gtgtcgcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nngatattcg ttttttattg atagggcaaa ggtagtaaag atgtatagaa tcgctcctgc
     3241 caattcctcc gctcctcatc gcttcaccca tttcttcacc ccaaatccct cctgcgtttg
     3301 caatctgaca aaatagacgc cttgcggcaa atggccggcc tctatactgc ggcgcaaggt
     3361 accttcgggc gcctcccaga tgccgccggc tactgcccgt ccgttcacat ctgtcaattg
     3421 ccaatgcacc gtggcgggcg catccaactg catttcaact tgtaccaagt cccaaccggg
     3481 attggggaag atacgcacgc ctgcattttc caaccagttc tca
//
LOCUS       DAORLF010000470         3521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14511_length_3521_cov_2.004905, whole genome shotgun sequence.
ACCESSION   DAORLF010000470 DAORLF010000000
VERSION     DAORLF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14511_length_3521_cov_2.004905"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1618
                     /locus_tag="PK198_24150"
     CDS             62..1618
                     /locus_tag="PK198_24150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF41916.1"
                     /translation="MKEPAKRIVGLVLLAAALWPLFYKLGDAPLRAWDESLFAMRAYY
                     AVEEGGYLPNFDYYPGITFYRNLKPPFGTWWQALSYRAFGYDEWTLRLPIALFSALLV
                     GMLWFWRRAVPDLPYSGVAAGWILVLSPGFLRDHVARTGDHDAILLFLMTAGLLLVYL
                     TERTYAAGQVQRRRWYLGLLGLTLLAGFLTKSFFAFALLPAFALWFLWRKRLVSLLRE
                     PMLWAAAALVMGGVVLYYALMERAFPGFMPFEQDTVLGRYVKVQDGHQLSWNYYISEF
                     FSRRFWPWWGVLLLHGGLMFRIQNFGFRIPDSGLHSRPPTDSGFRIPDSGLHRRPPTD
                     SGFRTFGALLWLSLLVHFVMITFSVTKLSWYDAPLYPIAALMAGSAVQALAEEFPDWK
                     KMIWIVLALLLAGFSVRALRDISQTGPASDDERYQPFMEYLQQKRPDIKAYKVYCHEY
                     SGQTGFTARLLNDQHGYNISVAAYYPEERFAGGDYILVCSAEKEERIYKEHVVEVMEE
                     SGGCKLLLLK"
     gene            complement(1622..2698)
                     /locus_tag="PK198_24155"
     CDS             complement(1622..2698)
                     /locus_tag="PK198_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HRF41917.1"
                     /translation="MPLRPTDRRQNKRRFRLRLPQRRLKLHIALWLLVSEILIGTVGF
                     MILEGYDPIEAFYMVIITISTVGFTEVEPLHPLGRIFTSMLILFNIGIFAYLLAVISY
                     YLVEGEGFKKWYLRRMKKSIDALQGHVILCGFGKYGKEISLNLLHHDLPFVILEKALD
                     KIEKIQSNDPPLLWLEGDATRDELLIEAGIQRAKAIICALADDSDNLFIVLSARQLAP
                     KINIISRAMDPRSEKKLMMAGANHVVMPEQIGGFYMATLVSKPGAVEFFSFITREYQS
                     DIRFEEIQFADLPEPYRHIAMSDLHLRARTGVNVIGHRDKNGSYHVNPVPETILAPDE
                     SFIVLGSEEQLESLHRMLEEEYKE"
     gene            2837..>3521
                     /locus_tag="PK198_24160"
     CDS             2837..>3521
                     /locus_tag="PK198_24160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF41918.1"
                     /translation="MRNNTIRNISIFAALAVLVGGGLLYRSGFFGGEQELETAAAPAK
                     PGGPGGMGGSGGPGGPGGAPLSVSALKIKTDILRDEISVNGSTASPEEVLVSAEVAGK
                     ITRILFSEGNYVNKGALLVQLDDEELQAQRKRLLVRKELTQRIAERLKNLYDREGVSL
                     QEYEIARAEADQVLAEITLIDVQISKRSIRAPFSGLLGLKMVSEGSYVSPGMAIVKLV
                     STNPIHLSFT"
BASE COUNT          713 a          892 c         1054 g          862 t
ORIGIN      
        1 tgaaaacctt cgcgccattt ccgccaaaac tcctactttc atggcctcaa actgagtatg
       61 tatgaaagag ccggccaaac gcatagtggg ccttgtattg ttggcggcag ccttgtggcc
      121 tttgttttac aaattgggcg atgcgccgct gcgcgcctgg gacgagtcgc tgtttgccat
      181 gcgcgcctac tacgcagtgg aggaaggcgg ctacctgccc aatttcgact actaccccgg
      241 catcacgttt tatcgcaacc tcaaaccgcc cttcggcacc tggtggcagg ccctgtcgta
      301 ccgcgctttt gggtacgatg agtggacctt gcggctgcct atagccctgt tttcggcctt
      361 gctggtgggt atgctctggt tttggcggcg ggctgtgcct gacctgccct attcgggtgt
      421 ggcggcgggt tggatactcg tgttgtcgcc gggtttcctg cgcgatcatg tggcccgcac
      481 cggcgatcac gacgccattt tgttgttttt gatgacggcc ggtttgctgt tggtgtatct
      541 gacggaaaga acttatgcgg ccggacaggt gcaacggcgg cggtggtatc tggggctgct
      601 tggtcttact ttgctggccg gatttctgac caagagcttt tttgcgtttg cgctgctgcc
      661 ggcttttgcg ctgtggtttt tatggcgaaa gcggctcgta tcgctgctgc gcgaacccat
      721 gctgtgggcg gcggcggcac tggtgatggg cggcgtggtt ttgtattatg cgctgatgga
      781 gcgggccttt ccgggcttta tgccttttga acaggatact gtgctgggca ggtatgtgaa
      841 agtgcaggac gggcatcagt tgagttggaa ttactacatc tccgagtttt tcagtcggcg
      901 tttttggccc tggtgggggg tgttgctgct gcatgggggt ttgatgtttc ggattcaaaa
      961 tttcggattt cggattccgg attccggatt gcacagccga ccgccgactg attccggatt
     1021 tcggattccg gattccggat tgcaccggcg accgccgact gattccggat ttcggacgtt
     1081 tggcgcgctg ctgtggctga gtttactcgt tcattttgtg atgattacgt tttccgttac
     1141 caagttatcg tggtacgatg cgcccctgta ccccattgcg gcgctgatgg cgggcagtgc
     1201 ggtgcaggct ttggcggagg agtttcccga ttggaaaaaa atgatctgga ttgttttggc
     1261 gctgctgttg gcgggttttt cggtgcgggc gctgagagac atttcgcaga ccggtccggc
     1321 ctccgatgat gagcgctatc agccgtttat ggagtatttg caacaaaagc gaccggacat
     1381 caaggcgtac aaagtctatt gtcacgaata cagcggccaa acgggcttca cggcgcgcct
     1441 gctgaacgac caacacggct acaacatctc ggtggcggcg tactacccgg aggagcggtt
     1501 tgccggcggg gattacatct tggtttgcag cgccgagaag gaggagcgca tttataagga
     1561 acacgttgtg gaggtgatgg aagaaagcgg ggggtgtaag ttgttgttgt tgaagtaatt
     1621 gtcactcctt atactcctcc tccagcatcc ggtgcaaact ctccaactgt tcctcgctcc
     1681 ccaaaacgat gaagctctcg tccggcgcca ggatggtttc cggcacggga ttgacgtggt
     1741 agctcccgtt tttgtcgcgg tggccaatga cattgacgcc ggtgcgggcg cgcaggtgca
     1801 ggtcgctcat ggcgatgtgg cggtaaggct caggcaggtc tgcgaactgg atttcctcga
     1861 accgaatatc cgattgatac tctctggtga tgaatgaaaa gaactccaca gcgccgggtt
     1921 tgctgaccaa agtggccatg taaaagccac cgatttgctc cggcatgacc acatggttgg
     1981 cgccggccat catcagtttt ttctccgagc gggggtccat ggcccggctg atgatgttga
     2041 ttttgggggc caactgacgg gccgacagca caataaacag attgtcggaa tcatcggcca
     2101 gggcgcagat gatggctttg gcgcgttgaa tgccggcctc gatgagcagt tcgtcgcggg
     2161 tggcatcgcc ttccagccag agcaggggcg ggtcgttgct ttgtatcttt tcaattttgt
     2221 cgagggcttt ttccagaatg acaaaaggca ggtcgtgatg gagcaaattg agcgaaattt
     2281 ctttgccgta cttgccgaac ccgcacagga tgacatgacc ctgcaaagcg tctattgatt
     2341 ttttcatgcg tcggagatac cattttttga agccctcgcc ttctaccaga taatatgaaa
     2401 tgaccgccag cagataggca aagatgccga tgttgaacaa gataagcatg gaggtaaaaa
     2461 tacgccccag aggatgcagc ggctctacct cggtgaatcc caccgtggaa atggtgatga
     2521 tgaccatgta aaaagcctca atcgggtcgt atccctccag aatcataaaa cccactgtgc
     2581 cgatgagaat ttccgacact aacagccata aagcgatatg gagtttgagc cggcgctgcg
     2641 gtaagcggag ccggaaacgg cgtttgtttt ggcgtcggtc ggttggcctc agtggcatgg
     2701 aattgttgct tatccttttg cctgcaaaaa taactcattg aaccaaagta aacgagggga
     2761 agttaccaca actgaaaaac aggctgaata tgcttgtttt attaccttta cgcactcatt
     2821 catcatctca tatccaatgc gcaacaatac catcagaaac atttccattt tcgctgctct
     2881 ggccgtactc gtcggcggag ggctgctgta tcgttcgggc ttcttcggcg gcgaacagga
     2941 gttggaaacg gccgcggctc cggccaaacc cggcggaccc ggcggcatgg gcggttccgg
     3001 aggaccgggc ggacccggcg gcgcgcccct gagtgtcagt gctttaaaaa taaaaaccga
     3061 catcctgcgc gatgaaattt ccgtcaacgg ctccaccgct tcgcccgaag aggtgctcgt
     3121 ttcggcagaa gtggccggca agatcacccg cattttgttt tccgaaggca attatgtcaa
     3181 caagggcgct ctactggtac aattggacga cgaggaactc caggcccagc gcaaacgcct
     3241 gttggtgcgc aaagaactga cgcagcgcat tgccgaacgt cttaaaaacc tctacgaccg
     3301 cgagggcgtg agtttgcagg aatacgaaat tgcccgcgcc gaggccgacc aggtgctggc
     3361 cgaaatcacc ctcattgacg tgcagatcag caagcgcagc atccgggcgc cgttcagcgg
     3421 gctgctgggc ctgaaaatgg tgagcgaggg cagctatgta tcgccgggca tggccatcgt
     3481 caaattggtg agtaccaacc cgatacacct gtcgtttacg g
//
LOCUS       DAORLF010000471         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14529_length_3516_cov_1.867090, whole genome shotgun sequence.
ACCESSION   DAORLF010000471 DAORLF010000000
VERSION     DAORLF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14529_length_3516_cov_1.867090"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>297
                     /locus_tag="PK198_24165"
     CDS             <1..>297
                     /locus_tag="PK198_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRF41919.1"
                     /translation="GVVVTMVWQQLYGLETGLLNRALIALHLPRVEWLSNPYMAMISI
                     AVMATWKNVGLYVILFLTGLQTVPRSYYEAAEVEGASAWQQFRYITLPAINPTLF"
     assembly_gap    298..425
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            518..>738
                     /locus_tag="PK198_24170"
     CDS             518..>738
                     /locus_tag="PK198_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRF41920.1"
                     /translation="MKAPKLFFYILLAGASLSFLYPFYWMLLATLTPEAQIGALGFLP
                     AGLTMDNYQLLADKIPIGRALLNSFIVAA"
     assembly_gap    739..915
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <916..1342
                     /locus_tag="PK198_24175"
     CDS             <916..1342
                     /locus_tag="PK198_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRF41921.1"
                     /translation="DTLAALVIPALNSAFAILLFRQAFQSIPDDLIHAARVDGCSELR
                     IIFQILLPNVIPAVITAGIITFMNSWNDVLWPLIVIRNEQLMTMPQLVALFAVGGRSE
                     AQLGVKLAAAVLLALPIIVAYAVFQRHFIQSMASSGLKG"
     gene            1373..2626
                     /locus_tag="PK198_24180"
     CDS             1373..2626
                     /locus_tag="PK198_24180"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRF41922.1"
                     /translation="MIHVKRSHHRLLADPRKVLPRFLNMGSPERIEPVAGHVAALSET
                     ETHRLLTELRDEFEARHFDLPSLWLEHFHKIARFVPDKLSESQQMLLGAYFTHEYSIQ
                     ASALFNPSIVPHPDQSGLTEDELRFILSLRAVGEGHISSIAFQTGVADREGNITLDET
                     QPQLTQGRILGKDSDLENGYDLEFPEASPLSSRVLFPVSAAESNGMEDARFVRFNDGE
                     RPRYYATYTAYDGRRIRPHWAETEDFRRFSIRPLSGRAASDKGMAIFPEKVNGRYAMI
                     GRQGGRCMSIMYSDDLLHWDEHSLLQAPAQSWEMLQMGNCGSPLKTPQGWLLLTHAVG
                     PMRKYVLSLSLLHPEHPERVLASLPYPLLQPNADEREGYVPNVLYTCGMILHEGNLII
                     PYAMSDSAIGFASAPIDEVLRELIR"
     gene            2631..>3516
                     /locus_tag="PK198_24185"
     CDS             2631..>3516
                     /locus_tag="PK198_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41923.1"
                     /translation="MIVRILLAFSLLILASCGSRTDLVNTRHLEHLYEEKTLDGTTMG
                     CIWIYCEAPDYHLVADEDEGFTCVDDVARALVFYCRDYAARPDPSTLRKIRSLAEFVL
                     FMQADNGYFYNFMFQNGDINTTHQNSVPVPAFWAWRALWALSELNLLETAELAELQNR
                     SRRAMEKLIKNARSLCVNESVYAEFEGVKVPACLAELGADQAGVMLLALSNHYRKYPS
                     ELVKELMLRLGTLLLETQYGDADTPPHFAFFSWRNYWHAWGNSQAYALLRAGRLLDHP
                     AFVEAALNEVRHFYPYVLK"
BASE COUNT          681 a          954 c          850 g          726 t
ORIGIN      
        1 ggcgtggtcg tcaccatggt gtggcagcag ttgtatggtt tggaaaccgg cctgctcaac
       61 cgcgccctca tagccctcca cctcccccgc gtggaatggc tctccaaccc ctacatggcc
      121 atgatctcca tcgccgtcat ggccacctgg aaaaacgtgg gcctgtacgt cattttgttt
      181 ttgacaggct tgcaaaccgt gccccgcagc tattacgagg ctgccgaagt ggagggcgcg
      241 tcggcctggc agcaatttcg ctacatcacc ctgcccgcca tcaatcctac cctgtttnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnngctat tccgccaccc gggggctgtt cttcgcgctg ctgatcatgt cggttgtggc
      481 agttcaaaaa ttggtcatcg aaaaagaatc ctgacacatg aaagcgccca aactcttctt
      541 ttacatactg ttggcgggag cctcgctctc gtttttgtat ccgttttatt ggatgctgct
      601 ggccacattg acgcccgaag cacaaatagg agcgctgggc ttccttcctg ccggcctgac
      661 aatggacaac taccaattgc tggccgacaa aatccccatc ggcagggcct tgctcaacag
      721 cttcatcgtg gcggccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnncgaca cgctggcggc cttggtcatt ccggcgctca acagcgcgtt
      961 tgccattttg ctgtttcgcc aggcatttca aagcatcccc gacgacctca tccatgccgc
     1021 gcgggtggac gggtgcagcg aactgcgcat catttttcaa attctcctac ccaatgtcat
     1081 accggccgtc attactgcgg gcatcatcac ttttatgaac tcctggaacg acgtgctctg
     1141 gccgctcatc gtcattcgca atgagcaact gatgacgatg ccgcaattgg tggctttgtt
     1201 tgccgtgggc ggcagatccg aggctcaatt gggcgtcaaa ctggcggcgg cggtgctgct
     1261 ggcattgccc atcatcgtgg cgtatgctgt gtttcagcgg cattttattc aaagcatggc
     1321 ctcctccggc ttgaaaggct gaggcgcatc ccacactctc aaacatctcc acatgattca
     1381 tgtaaaaaga agtcatcacc gccttctggc cgatccccga aaggtgctgc cccgctttct
     1441 caatatgggc agcccggagc gcatcgaacc ggtagcaggc cacgtcgcgg cactgagcga
     1501 aactgaaacg cacaggctcc tgacagaact ccgagacgag tttgaagccc ggcatttcga
     1561 cctgccctcg ctgtggctcg aacatttcca caaaatagcc cgcttcgtac ccgataagct
     1621 cagcgaatcc caacaaatgc tgctgggcgc ttatttcaca cacgagtaca gcatacaagc
     1681 ctcggccctg ttcaacccgt ccattgtgcc gcaccccgac caaagcggcc tgaccgaaga
     1741 cgagctgcga ttcatcctgt cgctgcgggc agtcggcgag ggacatattt cttccatcgc
     1801 ttttcaaacg ggcgtggccg accgcgaggg gaacatcacc ttagacgaaa cgcagcctca
     1861 actcacacag ggcaggattt tgggtaaaga cagcgacttg gaaaacggct acgatctgga
     1921 atttcccgaa gcatcgccct tgagcagccg ggtcttgttt ccggtctccg ccgccgaatc
     1981 gaacgggatg gaagacgccc gctttgtgcg cttcaacgat ggggaacggc cccgatacta
     2041 cgccacttat acggcctacg acggcagacg catccgaccg cattgggcgg aaacagagga
     2101 cttccggcgg ttttccatcc gaccgctttc cggacgggcc gcctccgaca aaggcatggc
     2161 catttttcct gaaaaagtga acggccgcta tgccatgatc ggcaggcagg gcgggaggtg
     2221 catgtccatc atgtattccg acgacctgct ccactgggac gagcacagtc ttttgcaggc
     2281 gcccgcacaa agctgggaaa tgctgcaaat gggcaattgc ggcagcccgc tgaaaacgcc
     2341 gcagggttgg ttgctcctca cacatgccgt aggtcccatg cgcaagtatg tgctgagcct
     2401 gagcctgctg cacccggagc atcccgaacg ggtgttggcc tccctaccct accctttgct
     2461 gcaacccaat gcggatgaac gcgaaggtta tgtgcccaac gtactctaca cctgcggcat
     2521 gatcctgcac gagggcaacc tgatcattcc ctacgccatg tctgacagcg ccattggttt
     2581 tgcttccgcg ccgatagatg aagttcttcg agaattgata agataaaaat atgatagtca
     2641 gaatcttact tgctttttca ctcctcatcc tcgcctcctg cggcagccgt accgaccttg
     2701 tcaatacccg gcatctggag catttgtacg aggagaaaac cctcgacggc acaacgatgg
     2761 gctgcatctg gatttactgc gaagcgccgg attatcactt ggtagccgac gaagacgagg
     2821 gctttacctg tgtggacgac gtggcccgcg cattggtctt ttattgccgg gattatgctg
     2881 cccgccccga tccctcaacg ctgcgaaaaa tccgctctct ggcggaattc gtgctgttca
     2941 tgcaggccga caatggctat ttttacaatt tcatgttcca aaacggagac atcaacacga
     3001 cacatcaaaa cagtgtgccc gtaccggctt tttgggcctg gagggcgctg tgggcgctgt
     3061 cggagttgaa tttgctggaa acagccgaac tcgccgagct tcagaatcgc agccgccggg
     3121 ccatggaaaa actgattaaa aatgcccgct cattgtgtgt aaatgagtcg gtatacgctg
     3181 aatttgaggg cgtaaaagtg cctgcctgtc tggccgaatt gggcgccgac caagcgggcg
     3241 taatgctgct ggcgctgagc aatcactacc gaaagtaccc ctcggaactg gtgaaagaac
     3301 tgatgctccg cctcggtacg ctcctgctcg aaacacaata tggcgatgca gatacgccgc
     3361 cacattttgc atttttcagt tggcgcaatt actggcatgc ctggggcaac tcgcaggctt
     3421 atgcgctgct gcgggctggt cgtctgctcg atcacccggc ctttgtggag gctgccctga
     3481 acgaggttcg gcatttttac ccttatgttt tgaagg
//
LOCUS       DAORLF010000472         3515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14533_length_3515_cov_3.744798, whole genome shotgun sequence.
ACCESSION   DAORLF010000472 DAORLF010000000
VERSION     DAORLF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14533_length_3515_cov_3.744798"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PK198_24190"
     CDS             <1..1202
                     /locus_tag="PK198_24190"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF41924.1"
                     /translation="TPNFTENAVRFIHTLTSIPDVQLGLITEEPVVLLRHDLSARLYG
                     HRQVANPLDPKLLEQAARELTAQHGPIHRIIGAVEIMQVPLAQVREALGIEGMGVETA
                     LNFRDKARMKAKLREAGIPCARYQTVASPAEARTFAELCGFPLIAKPPAGMGSLSTYK
                     IHNHHELDAVLREMPPSPGHEVLFEEFVTGTEHSFDTFSLNGQAVFHSLTHYHPNPIE
                     VMREPWIQWQVVLPREIDVPEYDDIRQAAFKTLDVLGMKTGLSHLEWFRRPDGSVAVS
                     EVAARPPGAQITTLMSRANDFDSLDAWARMIIFGEFKAPERKYAVGAAYLRGMGSGFI
                     KAVHGLEKVDREVGHLITDAKIPEVGRPHEGNYEGVGYIILRHPETHVVEAALKKVVS
                     LVRIELS"
     gene            complement(1295..2074)
                     /locus_tag="PK198_24195"
     CDS             complement(1295..2074)
                     /locus_tag="PK198_24195"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRF41925.1"
                     /translation="MEWFLQNPERHMYTMSAIYAEFGISRQGHWQALEREEALMAKEL
                     CYVGLMQEVREMHPGMGLRKMYEQFSPQGIGRDAFIALGLQEGFRLCAPINPQRTTRS
                     VKSARYRNLLQGKKFTNVNQIWVSDLFYFALGNLIHHSDRGGQYISDDYTNLLEDYKI
                     QISMCTDVLENAHSERVNGTIKNEYLARWHIANAEHLQQRLPTAINNYNDRRHDSLGM
                     TPNEFETYVKELSAGQRPELEIFTINKSFENSTQLTLNFDS"
     gene            complement(2029..2451)
                     /locus_tag="PK198_24200"
     CDS             complement(2029..2451)
                     /locus_tag="PK198_24200"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF41926.1"
                     /translation="MANKENKKFQLQMRSTRTFSTEFKKEKVKDLIEKRTSIKMLVEL
                     YGVSRSAIYKWLYLYSPMEKGIQTVVQMESEEQKTKVLLNRLAECERIIGQKQMQLDV
                     LEKTFEIASEELGYDVKKKYAPGPWNGSCKTLSDTCTP"
     gene            complement(2536..>3515)
                     /gene="kdsA"
                     /locus_tag="PK198_24205"
     CDS             complement(2536..>3515)
                     /gene="kdsA"
                     /locus_tag="PK198_24205"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRF41927.1"
                     /translation="LARGSTFMFTTHGVVRIIRRVLPAFINSPFFRSREVLPRADYPA
                     GSTRFHQLTIMIEIPVPKLRHTDSNNFFLIAGPCAVESEDIVLRTAEHLAELCDRLRI
                     PLIFKSSYRKANRSRLDSFAGIGDEQALRILRKAAETFDLPVTTDIHADDEAAFAAEY
                     VDVLQIPAFLCRQTSLLVAAAETGKAVSVKKGQFVSPESMRFAVDKVVQSGNDRVMLI
                     ERGATFGYQDLIVDFRGIPVMQSFGVPVILDCTHSLQQPNQASGVTGGQPALIETIAR
                     AGVAVGVDGLFIETHPNPAEAKSDGANMLPLHQMEKLLERLLRIRQAVL"
BASE COUNT          775 a          906 c          969 g          865 t
ORIGIN      
        1 ccacgccgaa ttttacggaa aatgccgttc gctttatcca cacgctgacg agcatccccg
       61 atgtgcagct cgggttgatc accgaagagc ctgttgtttt gttgagacac gatttgtcgg
      121 cccggctgta tgggcaccgg caagttgcca atcctttgga tccaaaatta ttggaacagg
      181 ctgcccgcga actgaccgca caacacggcc ccatccaccg gatcataggg gcggtagaga
      241 tcatgcaggt accgctggcc caagtgcgcg aggcattggg aatagaaggc atgggcgtag
      301 aaacggcgct gaatttcaga gacaaagccc gcatgaaagc caaactccgc gaggccggaa
      361 ttccctgcgc ccgctatcaa acggtggcaa gccctgccga ggcccgtact tttgcagagt
      421 tgtgcggatt cccgctcatc gccaaaccgc cggcgggcat gggatcgctt tctacttaca
      481 agattcacaa tcaccacgaa ttggacgccg tgctgcggga gatgccgcct tcgccgggcc
      541 atgaggtgct ctttgaggag tttgtgacgg gcaccgaaca ttctttcgac actttttcgc
      601 tcaatggcca agcggttttt cactcgctga cgcattacca ccccaatccc atagaggtga
      661 tgcgcgaacc ctggattcag tggcaggtgg tattgccccg tgaaattgat gtgcccgaat
      721 acgatgatat tcgtcaggcg gccttcaaaa ctttggatgt gctgggaatg aaaaccggat
      781 tgagccattt agagtggttt cggcgccccg acggcagcgt ggcagtgtcg gaagtggcgg
      841 cgcggcctcc cggagcgcag atcacaacgc tcatgtcgcg tgccaatgat ttcgacagct
      901 tggacgcctg ggcgcgcatg attattttcg gcgagttcaa agcgccggag cgcaaatacg
      961 ccgtaggtgc cgcttacctc cggggtatgg gcagcggttt tatcaaagcg gtacacggtc
     1021 tggaaaaagt ggaccgtgag gtgggccatc tcatcacaga tgccaaaata ccggaagtgg
     1081 gccgcccgca tgagggcaat tatgaggggg tcggctacat catcctgcgg cacccggaaa
     1141 cgcatgtggt ggaggctgca ctgaaaaaag tggtgtcgtt ggtaaggata gagttgtctt
     1201 aatcagtcgg cggtatgcgg tgtgcagtag gcggtttgcc gtcggcggta ggcggtgtgt
     1261 gcatgcctga tataggttga cggttgtgag tgaattatga atcaaaattt aacgtaagtt
     1321 gcgttgaatt ttcaaaagat ttgtttatag taaatatttc aagttcaggt ctctgaccgg
     1381 cagaaagttc cttgacataa gtttcaaatt cgttgggtgt cataccaaga gaatcgtggc
     1441 gccggtcgtt gtaattattg atggccgttg gaaggcgctg ctgtaagtgc tctgcgttgg
     1501 caatgtgcca ccgggcgaga tactcattct tgatggtgcc gtttacccgt tcactgtgtg
     1561 cgttctctaa gacgtcggta cacatactga tctgaatttt atagtcctcc aggaggttgg
     1621 tataatcatc gctgatatac tggccgcccc gatctgaatg atgaatcagg ttgcctaaag
     1681 caaaataaaa caaatcgctg acccatatct ggttgacatt tgtaaacttc ttgccttgca
     1741 acaggttcct gtatcgcgcg cttttgacgc ttctggtggt gcgttgcggg ttgatcggcg
     1801 cgcacaagcg gaagccttcc tgtagtccca atgctataaa ggcgtctcga ccaatgccct
     1861 gaggactgaa ctgctcatac attttgcgca ggcccattcc gggatgcatc tcccgcactt
     1921 cttgcatcag gcccacatag cacaattctt tggccatcaa cgcctcttcg cgctctaagg
     1981 cctgccagtg gccctgtctg ctaataccga actcggcgta tatcgcactc atggtgtaca
     2041 tgtgtcgctc agggttttgc aggaaccatt ccacggtccg ggtgcgtact ttttttttac
     2101 gtcataccca agctcctcgc tcgctatttc gaaggtcttc tccaacacat ctaattgcat
     2161 ttgtttctga cctatgatcc gctcgcattc cgccagccga ttgagcagta ccttcgtctt
     2221 ttgttcttcg ctttccattt gcactactgt ttggattcct ttctccatgg gcgagtacaa
     2281 atatagccat ttatagatcg cagagcgact tactccatag agttcaacaa gcatctttat
     2341 gctcgtccgt ttttcgataa ggtcttttac cttctctttc ttgaactccg tgctgaatgt
     2401 ccgagtggac ctcatctgaa gttgaaactt tttgttctct ttgtttgcca tctttgattg
     2461 acttttttgt caacctattt taggcacgca catactgcct actgcctatc gcccaccgcc
     2521 caccgatccc ccggctcaaa gcacggcctg ccgaatgcgg agcaatcgtt cgagcagttt
     2581 ttccatctga tgcaaaggca acatattggc gccgtctgat ttggcttccg ccggattggg
     2641 atgcgtttcg atgaacagtc cgtccacgcc aacggccacg ccggcccgcg caatggtctc
     2701 gatgagcgcc ggttggccgc ctgtgacgcc cgatgcctga ttgggctgtt gcagagaatg
     2761 agtgcaatct aaaataacgg gcacgccgaa agactgcatg accggaatcc ctctgaagtc
     2821 cacgatcaaa tcctgataac cgaaggtggc gccgcgctcg atgagcatca cgcggtcgtt
     2881 gccggattga accactttgt caacagcaaa acgcatactt tcggggctga caaactggcc
     2941 ctttttgacg ctgacggcct tgccggtttc ggccgctgct accaacaggc tggtttgacg
     3001 gcaaaggaag gccggtattt gcagcacatc cacatactcg gcagcaaagg cggcttcgtc
     3061 gtcggcgtgg atgtcggtgg tgacaggcag gtcgaaagtt tcggcagctt tgcgcaaaat
     3121 gcggagggcc tgttcgtcgc cgatgccggc aaaagaatcc aatcgggaac ggttggcttt
     3181 gcgatacgaa cttttaaaaa tcaagggaat gcgcaggcgg tcgcacagtt ctgccagatg
     3241 ttcggcggtg cgcaggacga tgtcctcact ttctacggcg caggggccgg caatgaggaa
     3301 gaaattgttg gaatcagtgt gacggagttt gggaacggga atctcaatca tgatggtaag
     3361 ttgatgaaag cgggtagaac ccgccggata gtccgcacga ggtagaacct cgcgcgagcg
     3421 aaaaaatggt gagttgatga aggcgggtag aacccgccgg ataatccgca ccacgccgtg
     3481 cgtggtaaac atgaaggtag agcctcgcgc gagcg
//
LOCUS       DAORLF010000473         3466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14803_length_3466_cov_2.368220, whole genome shotgun sequence.
ACCESSION   DAORLF010000473 DAORLF010000000
VERSION     DAORLF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_3466_cov_2.368220"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1370
                     /locus_tag="PK198_24210"
     CDS             9..1370
                     /locus_tag="PK198_24210"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRF41928.1"
                     /translation="MGFPESRGILSMMTAVLDRHYPGKNMSQMEQELQSLLDDPGAFA
                     EHPNWSEVAHRLLPPPATAVLSENKSPASLSIFGAEYIDAGALNQMYTALRLPVSVAG
                     ALMPDAHSGYGLPIGGVLATENAVIPYGVGVDIGCRMCLSIYDLPTDMVLRDKDKLTQ
                     ALMDHTVFGAGGECAKNRRSDHEILSDSRFGDLPMLKQLKDLAYKQLGTSGSGNHFVE
                     FGVLEVSGEHLGAKVPPGTYLALLSHSGSRGLGATIAGHYTALAIKRCPLPDKAKHLA
                     WLPLDTDEGREYWLAMNLAGDYASACHDLIHRRIAAALRTQPLLRVENHHNFAWKEIH
                     DDRELIVHRKGATPAGQGVLGIIPGSMAAPGYLVAGRGQSNSLNSAAHGAGRQMSRSE
                     AKNRITPKMMAQTLAAKGITLIGGAVDEAPEAYKDIGQVMQAQADLVDVVGRFQPKLV
                     RMA"
     gene            1437..2381
                     /locus_tag="PK198_24215"
     CDS             1437..2381
                     /locus_tag="PK198_24215"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRF41929.1"
                     /translation="MSNSIFETNYSFPGQTAFYRGKVRDVYSIGDLLVMIASDRISAF
                     DHILPRPILHKGQVLNQTAVHFLEATRDIVPNWLLASPDPVAAVGIRCEPVRVEMVIR
                     GYLSGHAWREYKAGKRVLCGVAMPDGLRESDPFPAPIITPATKAEEGHDEDISREEIL
                     RQGIVSEEDYLQMEAYTRALFQRGTEMAASHGLILVDTKYEFGKKDGKIYLIDEIHTP
                     DSSRYFYADGFDERQASGERQQQLSKEFVREWLMANGFQGFDNQEMPAMPDAFVEEVS
                     ARYIALYERVTGQNFVKADLTNVQQRIQDNVCRFLETV"
     gene            2571..>3164
                     /locus_tag="PK198_24220"
     CDS             2571..>3164
                     /locus_tag="PK198_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41930.1"
                     /translation="MLYAKGTRVRLRHSAEQGVVTEILDKDLLMIRLDGEDMEIPVFA
                     EDLENNWSEKTAGVKAKVITSKSNSDTSTPAAFIPAESQYAILKSVGIQLAFDAESGG
                     VFNMHLLNDTDREYIFHFQLITLHKKSPEIHGKLPAVSAVPVGNMLLDDLNDQPEIHI
                     ACSRITTGGLEPGMEKVLKIKAKQFFSKTLTAPILNRP"
     assembly_gap    3165..3234
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          836 a          889 c          905 g          766 t
ORIGIN      
        1 tcaagcgcat gggtttcccc gaatcgcgcg gcatattgag catgatgacg gctgtattgg
       61 accggcacta ccctggaaaa aatatgtcgc aaatggagca ggaactccag tcgctgcttg
      121 atgatccggg cgcgtttgcc gagcatccta actggagcga agtggcgcat cgcctgctgc
      181 cgccccctgc tactgcggtc ctgtcggaga ataaaagtcc cgcttccctt tccattttcg
      241 gcgccgagta catagatgcc ggcgcgctca atcaaatgta cacggctttg cgccttcccg
      301 tcagcgtagc gggcgcgctg atgcccgatg cgcactccgg ctacggcctg cccatcggcg
      361 gcgtattggc caccgagaat gcagtgattc cctacggcgt cggggtggat attggctgcc
      421 gtatgtgcct gtccatctat gatcttccga ccgacatggt actccgcgac aaagacaaac
      481 tcacccaggc cctgatggac catacggtat tcggcgcggg cggcgagtgt gccaaaaatc
      541 gtcgcagcga ccatgaaata ctcagcgatt cgcgattcgg agatttgccc atgttaaagc
      601 agctcaaaga cttggcttac aagcaattgg gcacttccgg ttccgggaat cacttcgtgg
      661 agtttggagt actggaggta agcggcgaac atctgggcgc aaaagtaccg ccgggaacct
      721 atctcgcgct gctttctcat tccggctcgc ggggtttggg cgcaactatt gccggccatt
      781 acaccgcttt agccatcaag cgctgccccc tacccgacaa ggcgaaacac ttggcctggc
      841 tgcccttaga taccgacgaa ggccgcgaat actggttggc catgaatttg gcgggtgatt
      901 atgcctcggc ttgccacgac ctgatacacc gtcgcattgc cgccgccttg cgcacacaac
      961 cgctcctgcg cgtggaaaat caccacaatt ttgcgtggaa ggaaatacac gacgaccgcg
     1021 aactgattgt acatcggaaa ggtgctaccc ctgctggcca aggcgtgctg ggcatcatac
     1081 cggggtctat ggctgctccg ggctatctcg ttgcaggacg gggtcaatcc aattccctca
     1141 actcagcagc acacggagcg gggcggcaaa tgtcgcgcag cgaggccaag aaccgcatca
     1201 cacccaaaat gatggcccaa acgctggcgg ccaaaggcat cacgctcatc ggcggcgcag
     1261 tggacgaggc gcccgaagca tacaaggaca tcgggcaagt gatgcaggcg caggcagatt
     1321 tggtggatgt ggtagggcgc tttcagccca aattggtgcg catggcgtaa acagaagcga
     1381 caaaaaaacg gattttcaac ggagcacggt acctttgtcg cacaaaacgg caaagtatgt
     1441 ccaactcaat ttttgaaacc aattactcct tccccgggca aaccgcattt tacagaggca
     1501 aagtacgcga tgtctattcc atcggcgatc tgttggtcat gatcgcttct gatcgcattt
     1561 ccgcttttga tcatattttg ccgcgcccca ttctgcacaa agggcaggtg ctgaatcaaa
     1621 cagcggtgca ttttctggaa gctacccgcg acattgttcc caactggctg ttggcctccc
     1681 ccgatcctgt agctgccgtg ggtattcgct gcgagccggt acgggtggaa atggtcattc
     1741 ggggctattt gtcggggcac gcatggcggg agtacaaggc cgggaaacgg gtgttgtgcg
     1801 gtgtagcaat gccggatgga ttgcgcgaaa gcgacccttt tccggcgccg atcatcacgc
     1861 cggctaccaa agccgaagaa ggccacgacg aagacatcag tcgggaagaa atccttcggc
     1921 agggcattgt ttcagaggag gattacctcc aaatggaggc ttacacccgg gctttgttcc
     1981 agagaggtac ggaaatggct gcttcgcatg gattgattct ggtggatacc aagtatgaat
     2041 tcgggaaaaa ggacggaaaa atttacctca tagacgagat acataccccc gacagttcca
     2101 ggtattttta tgccgatggt tttgatgaaa gacaggcgag cggcgagcgg caacagcagt
     2161 tatccaaaga atttgtgcgc gaatggctga tggccaacgg ttttcagggg tttgataatc
     2221 aggagatgcc cgccatgccg gacgcttttg tggaggaagt gtcggctcgg tacattgctc
     2281 tttatgagcg cgttacaggg cagaattttg taaaagccga cctgacgaat gtgcaacagc
     2341 gcatacagga caatgtctgc cggtttttag aaactgtcta atcgccctcc cctactctac
     2401 ttgtttttca atacctttga agccttagta gtggggcaaa aataaatgct cattttcttt
     2461 aggccaaagg attagataag acacttaaaa tgaaccgttt acttatctaa tcctttgacg
     2521 aaaatgatca ctttattttt ttatcgccct ttaccctgaa attttgtacc atgctttatg
     2581 ccaaaggaac ccgcgtacga cttcgtcact ctgccgagca aggcgttgtg acggaaatat
     2641 tagacaaaga cctgctgatg atccggctgg acggcgagga tatggagatt cccgtgtttg
     2701 cggaagattt agaaaataat tggtcggaaa aaactgccgg agtaaaggcc aaagtaatta
     2761 ccagtaaatc aaattccgac acttcgactc ctgccgcgtt tattcctgcc gaaagtcagt
     2821 acgccattct gaaaagcgtg gggattcagt tggcttttga tgcagaatcg ggaggagttt
     2881 tcaatatgca cctgctcaat gatacagaca gagagtatat atttcatttt caactcatta
     2941 ccctgcataa aaagtcgccg gaaatacacg gtaaactccc cgcagtgagc gctgttccgg
     3001 ttggcaacat gttgctggac gacctcaacg atcagccgga aattcacatt gcctgctcca
     3061 gaatcactac cggcggactg gaacccggca tggaaaaagt gttgaaaatc aaggctaagc
     3121 agtttttttc caaaacattg acggcgccca ttctcaaccg gcctnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagccc
     3241 acgcgggaga tccagtccct cgccaccgac gcgaaccggc ccacgcctcc gcccgcatga
     3301 agtggaagaa ctggcgcatt ttgtaccaga aatagacctg cacatagagc aattgcttcc
     3361 caatgccaaa aaaatgagca acggcgaaat tgtaaaagta caattagctc attttgagcg
     3421 ttttttggac aaagcactcc gcgtgggcgc cgagcgggtt tacatc
//
LOCUS       DAORLF010000474         3460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14833_length_3460_cov_2.308957, whole genome shotgun sequence.
ACCESSION   DAORLF010000474 DAORLF010000000
VERSION     DAORLF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14833_length_3460_cov_2.308957"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK198_24225"
     CDS             complement(<1..614)
                     /locus_tag="PK198_24225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41931.1"
                     /translation="MKHAAVLFLLFIFSISLPAQDKYDYTWPNGYVTDISLNDSIQLQ
                     GGSLIHFGPHVERISYFRIPLYLSSYTFLCDQAGNLLFYATGCKVINARHELMENGDG
                     LYGGDNPNNNCQTSAYVTLQGEIALPSFPSQPDMYYILHLNTLQDDPPYYLSRDLQLT
                     TVDMSAAQGLGRVVEKNRVLYHNFMTDQLMAVRHANGRDWWVVVP"
     gene            complement(695..>907)
                     /locus_tag="PK198_24230"
     CDS             complement(695..>907)
                     /locus_tag="PK198_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41932.1"
                     /translation="GRVHFRLPANAAGRLLIYDMMGRSRFERSQTTDNLLGDVEVATS
                     GWQAGLYQAVFLPESGAAIVLPFILQ"
     assembly_gap    908..920
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<921..3369)
                     /locus_tag="PK198_24235"
     CDS             complement(<921..3369)
                     /locus_tag="PK198_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41933.1"
                     /translation="MTIGTDPNSVTNSSSLPAQYYTNQRIRINGTLIVNSPTFLLINC
                     RVQMGPGAVITVNEGKLLDGFKTQFFSCEQMWRGIHLKGAAKLRLISCVAEDAEYAIN
                     TESASVIALRENTFNRNYIGIRLRPASPEANILNFTYFRNNLFKGGSLLHSPYPGQQD
                     HSKLSYAGIDASECSFTVGDNIAPNTFTQIHRGITILDATVAVRNCRFTDLPEGLFTD
                     FGIYSEGGTLDVTWGGAEATRSQFINCGYGVLSYNTAMNIRYSSFSNLDIDGGLGIQS
                     VYNSMSTGGFFASYNNFQMDGIGTGIVTSRPFVPVGTGVDIFQNNFISQGTLGLGIKH
                     CISLFHSNNPTTNNLAYVRNNNFDIASNSNITYCTWGFNMGKNISITSNKYNFGASSG
                     QTFDTNDFAISVHGLEGGEISFNQIEGTASPGNFFRAVRAISVFDSPKIKICDNVVNN
                     TKAGLYFKGDNSQSNVSENTMGRHRIGLWVDGDNPMAGIGEQVRTNNRWSATPGDYID
                     WAAFCTQGVPPANSFFRVQSTDPVVYPSPISPMNGWFDPENGPLNSCIGAGENLLSDA
                     DQALMTGSIQLSPLQQWESEYRLLQKIQSRPAGTPIGLQTQTFVNQRAGTASGRLVLA
                     ERMMAQAMEAIEPWRDTLSAIQAYRSSIIDSLWAVEDALALPDTDNTLNEALALAKMP
                     LLYQLETLRLRERQIMSGVKAEMQPLFVDALNYNEATQVNQPWEGARKQLNSYLFRQW
                     MGIADSTDQNLLFSIALQNPNQAGEAVREARALITDPVWREIWAEHLTFPAGPSPQLA
                     MPGANAADGFRVWPNPAV"
BASE COUNT          856 a          847 c          884 g          860 t
ORIGIN      
        1 ggcactacca cccaccagtc gcggccattg gcatggcgaa cagccatgag ttggtcggtc
       61 atgaagttgt gatacaacac cctgtttttt tccactaccc tgcctaatcc ctgggccgcg
      121 ctcatgtcca ccgtggtgag ctgcagatcg cgcgagaggt agtaaggcgg atcatcttgc
      181 agggtattga ggtgcaggat gtaatacatg tcgggctgcg aaggaaaaga aggcagcgct
      241 atctcgccct gtaaagttac ataggcagat gtttggcaat tattattagg gttatccccg
      301 ccatacagcc cgtcaccgtt ttccatcagt tcgtggcgcg cgttgatcac cttgcagccg
      361 gtggcgtaaa acaacagatt tccggcttgg tcgcacagga aggtgtagct ggataaataa
      421 agagggattc ggaaatacga aatccgttcg acatgagggc cgaagtgaat cagggagcct
      481 ccctgtagct gaattgagtc attgagtgaa atatcagtta catacccgtt aggccatgta
      541 taatcatatt tatcctgagc cggaagggat attgaaaata taaaaaggag aaacagaacc
      601 gcggcatgtt tcatggcaat cactttttaa atgtgataca atgccggtag ccctttcgta
      661 ttcgtaaggg ctaccggaag gaaccttgat tctgttattg taaaataaat ggaaggacta
      721 tagcagcgcc ggattcgggc aggaacaccg cctgataaag ccccgcctgc cagccggagg
      781 tcgccacttc aacatcaccc aacagattat cggtggtttg gctgcgctcg aaacggctgc
      841 gtcccatcat gtcgtaaatc agcaggcggc cggcagcatt tgcgggcagg cggaaatgta
      901 cgcgcccnnn nnnnnnnnnn cgacggccgg gttgggccat actctgaacc cgtcggcagc
      961 gttggcgccc ggcatggcta gctgaggcga aggacccgcc gggaaagtca ggtgttctgc
     1021 ccatatttcg cgccacacgg ggtctgttat caatgcccgt gcttctctga ccgcttctcc
     1081 ggcctgattg ggattctgca aagcaataga aaacagcagg ttctgatcgg tgctgtccgc
     1141 tattcccatc cactggcgaa agaggtaact gttgagttgc ttgcgggcgc cttcccaggg
     1201 ttggttcacc tgcgtcgctt cattatagtt cagcgcgtcc acaaacagag gttgcatttc
     1261 agcttttact cccgacatga tctgtcgctc gcgcaggcgc agggtttcca actggtacaa
     1321 caaaggcatc ttggccagcg ccagcgcttc gttgagcgta ttgtctgtat cgggcagggc
     1381 caatgcgtct tccacagccc acaggctgtc tataatgctg ctccggtatg cttgtatggc
     1441 actgagggtg tcacgccacg gctcaatggc ttccatggct tgggccatca tgcgctccgc
     1501 cagcaccaaa cgccccgatg cagtgccggc gcgctgattt acaaatgttt gagtctgcaa
     1561 accaatgggc gttcctgccg ggcgggattg aattttttgc aaaagacggt attccgactc
     1621 ccattgttgc agcggagaca gttgaatact gcccgtcata agcgcctgat ctgcatcgga
     1681 gagcaaattt tcacccgcgc cgatacagga gttgagaggg ccgttttcag ggtcaaacca
     1741 gccattcatt ggagaaatcg gagatgggta cactaccggg tcggttgatt gcacccgaaa
     1801 gaaagaatta gcaggcggaa ctccctgcgt acaaaaagcc gcccaatcta tgtaatcacc
     1861 gggtgtggcc gaccagcggt tgttggtgcg cacttgttcc cctatacccg ccatggggtt
     1921 gtcgccatcc acccaaagcc caatgcggtg gcgtcccatg gtgttttcag aaacgttgga
     1981 ttgcgaattg tctcctttaa aataaagccc ggccttggta ttgttcacaa cattgtcgca
     2041 tatcttgatt tttgggctgt caaaaactga aatggcgcgg actgcacgga aaaagttacc
     2101 gggagaggcc gttccttcga tttgattaaa gctgatttct ccaccctcaa gcccgtgtac
     2161 actgatggcg aagtcatttg tatcaaaagt ttgcccgctt gaggctccaa aattgtactt
     2221 gttactggtt attgaaatat ttttccccat attaaatccc catgtacaat atgtaatgtt
     2281 actattgctg gctatgtcga aattattatt tcgaacataa gcaagattgt ttgtagtggg
     2341 gttattcgag tgaaacaggc taatacaatg tttaattccc agcccaagtg tcccctgtga
     2401 aatgaagttg ttttgaaata tatcaacacc ggtaccaaca ggcacaaagg ggcgagaggt
     2461 tacgatacct gtgccgatgc catccatttg gaagttatta taggaagcaa aaaaaccgcc
     2521 tgtggacatg ctgttgtaca cagactggat acccaatcct ccatctatat ccaagttgga
     2581 aaaagaactg tatctgatat tcatcgctgt gttgtacgac agcacaccgt aaccacaatt
     2641 gatgaattga cttctggtag cttccgcgcc cccccaagtt acatccagtg ttccgccttc
     2701 cgaataaatg ccaaaatcag taaagagacc ttccggcaaa tcagtaaagc ggcaatttcg
     2761 gacagcaacc gttgcatcca agatcgtaat tccccggtga atctgcgtga atgtatttgg
     2821 agcaatgttg tctccaaccg taaagctaca ttccgaagcg tctattcctg cataagaaag
     2881 cttgctgtgg tcctgctgcc ctggataagg actgtgcaaa agtgaaccgc ctttaaataa
     2941 gttatttcta aaataggtga aattgaggat attagcctcc ggcgaagcgg ggcgcaatcg
     3001 aatgccgata taattgcgat tgaaggtatt ctcccgcaaa gcaatgacgg acgcactctc
     3061 cgtattgatc gcatactccg cgtcctcagc tacacaggaa atgagcctca attttgccgc
     3121 gcctttcaaa tggatgccgc gccacatctg ttcgcaggag aaaaactgtg ttttgaaccc
     3181 gtccaacagt tttccttcat ttactgtaat gactgccccc ggccccattt gcactctaca
     3241 gttgattaaa agaaaggtag ggctgttgac aatcaaagtt ccattgatgc ggatgcgctg
     3301 attggtgtag tattgagcgg gaagcgagga actgttggtg acactattgg ggtccgtgcc
     3361 aatggtcaat tgcggtacaa agggcgtgtt tagctcacag agcgtttgtt cggtagtcag
     3421 ccaggtgatt gcggaattag agaatcctcc ggcaggcagg
//
LOCUS       DAORLF010000475         3456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_14851_length_3456_cov_2.407821, whole genome shotgun sequence.
ACCESSION   DAORLF010000475 DAORLF010000000
VERSION     DAORLF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_3456_cov_2.407821"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>319)
                     /locus_tag="PK198_24240"
     CDS             complement(<1..>319)
                     /locus_tag="PK198_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF41934.1"
                     /translation="NYRKAARSFEELSTVNSKLGQYAMYYLGDSYLRLDQKSSARTAF
                     ASAKRMNYDPAVQEDALFNYAKLSYELKDPREAITELQTIRPESRYYVDAQSLMSEIF
                     LGYR"
     assembly_gap    320..331
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..1228)
                     /locus_tag="PK198_24245"
     CDS             complement(<332..1228)
                     /locus_tag="PK198_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41935.1"
                     /translation="MTKRIALLLALYLGFSSLYAQSTAVFTEADLAYKRGMGFYKDGL
                     FGKAQEEFTQTMRLLQPANEPNAALLRTKAELHFAKSAVQLKLPDGEKLILDFIRKYK
                     PDPMAMQALTEMAKYYYDAKEYEKAISFYDQVDWSGLSKAEREEARFRLGYSYFVTKQ
                     FAKAKTQFREIRDVRNDYYEPTNYYLGLCYFFEGNYREAISSLKIAEKNNRFKDQIPY
                     YIAQIYFAERQYDELIAYAKPYASNTSGSRSKEIQQLLGQAYFEKGDYQSALPYLEFY
                     AERTGKLREEEFYQLGYAQYNTG"
     gene            complement(1469..>3456)
                     /locus_tag="PK198_24250"
     CDS             complement(1469..>3456)
                     /locus_tag="PK198_24250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF41936.1"
                     /translation="TPDCNDGCPNDPNKTAPGACGCGVADTDTDGDNTLDCNDGCPND
                     PLKTAPGACGCGVADVPTTWYADTDADTYGDPNNSIPGFTCNQPIGYVANNTDCDDNN
                     NAVNPGATEVVNGIDDNCNGMTDETGITYYRDMDGDGFGDPNNSILADSPPSGYVLDN
                     TDCNDDPATGGMINPGATEVCGNGIDDNCNGDIDEGCCPNLSTAPGEVLINNGTCGAA
                     CQPVGAGISAPTSGCPAGSTIQYSVNNGEWSNTLPLYANLSTGNIIRTRCSCDSDPLM
                     TGPASAGTPIGSCTDNTPPTITCASHATLNFNGQNSIQLDGMNGPVDFQIVANDNCGV
                     QSITITPSVINANQVGQIVPILITVTDQKGNTATCSTNLTVSGLPAGWSAPVNGIGCT
                     NGSDVDYNTATGVWTVASTNCYYGPNYTTDSEAFAQRTLCGDGSITALVTGINPLADG
                     WAGLVMRENVSAGAKKAQLMTNLNNLSRREFRTATNGAAVPQQFPSQNRFWLRIVRTG
                     HQFVLYVSPNGTTWHPAGSQSIVMQSCIEVGLVLTNYTSNSTVTGTFSNVGFTQVNPI
                     FSGQTQEQNTLGQVEFSLFPNPTSGELNLDLTQYIGRSVRIETYSLEGKLLQFTELDE
                     VQTTLERIDIKGLQNGMYLVKVKSVGLPDVTRRVVKQ"
BASE COUNT          766 a          832 c          951 g          895 t
ORIGIN      
        1 cgcggtagcc tagaaagatt tcactcatca ggctttgagc atccacataa tagcgcgatt
       61 caggccgaat ggtttgcagc tctgtgatgg cctcgcgggg gtctttgagt tcgtacgaaa
      121 gtttggcgta gttgaacaag gcgtcttcct gtacggcggg gtcatagttc atgcgcttgg
      181 ccgaggcaaa agccgtgcgg gccgaagatt tctggtctaa gcgcaggtac gaatcgccga
      241 ggtagtacat ggcgtactgg ccgagcttgg aattgacggt ggacaactcc tcaaaactgc
      301 gggcggcctt gcggtagttn nnnnnnnnnn ngcccgtgtt gtattgcgca tagccgagct
      361 ggtaaaattc ttcttcgcgg agcttgcccg tgcgctctgc ataaaattcg aggtagggca
      421 aagcgctctg ataatctcct ttttcaaaat acgcctggcc cagcaattgc tggatttcct
      481 tcgagcgcga gccggaagtg ttggaagcgt aaggctttgc ataagcaatc aactcgtcgt
      541 actgacgctc ggcaaagtaa atttgagcaa tataatacgg aatctgatct ttgaaccgat
      601 tgttcttttc agcaattttc aacgaactga tggcctcgcg atagttgcct tcaaagaaat
      661 aacacaaacc cagatagtaa ttcgtaggct cgtaatagtc gttgcgcacg tcgcgaattt
      721 cgcgaaactg cgtttttgct ttcgcaaatt gtttggtcac aaagtaggag taccccagcc
      781 ggaagcgcgc ttcttcgcgt tctgctttac tcaggccgct ccagtccacc tgatcgtaga
      841 acgaaatggc tttttcgtac tctttggcat cgtagtagta cttggccatt tcagtgaggg
      901 cctgcatggc catcggatcg ggcttgtatt tgcgaatgaa atccaggatg agtttttcgc
      961 cgtcgggcaa tttcaactga acggcgcttt tggcaaaatg caactcggct ttggtgcgca
     1021 agagggcggc attcggctcg ttggcaggtt ggagcaagcg catggtttgt gtaaactcct
     1081 cttgcgcttt tccgaaaagg ccatctttgt aaaagcccat tcctcgcttg taggccagat
     1141 cggcctcggt aaatactgcg gtagattggg cgtacaggct cgaaaaacct aagtacagcg
     1201 ccaacagcag ggcaattctt ttagtcatag aacagcttgt tttttgttaa ttcatgagca
     1261 agtggccgta aaacgcattt cacatctatt ctggtgtatg agcttgtttg aaatgcgtgt
     1321 tccggcagtt ttcagcggcg aaattaggaa gaaaatgtaa atctactttt gtcccgattc
     1381 atagttgccc tttttataaa aaacaggcaa acaaaaacgg ctgaagcatt gccgccccag
     1441 ccgttttttt acaaaaaaat caaatcattt actgtttaac aactcgtctt gtcacatccg
     1501 gcaggccgac agacttcact ttcactaagt acataccgtt ttgcaagcct tttatatcaa
     1561 tgcgttccag cgtagtttgc acttcgtcca attccgtaaa ttgcaacagc ttgccttcca
     1621 gactgtacgt ttcgatgcgc acgctgcggc cgatgtactg cgtcagatcg aggttcaatt
     1681 cgcccgaagt ggggttgggg aataaactga actctacctg accaagagta ttttgctctt
     1741 gtgtctggcc tgagaaaatg gggttcactt gtgtgaatcc aacgttggag aaggtgcctg
     1801 ttaccgtgct gttggaggtg taattcgtca acaccagacc aacttcgata caggattgca
     1861 tgacaataga ttgagaaccg gcagggtgcc atgttgtgcc attcggtgaa acgtacaaaa
     1921 caaactgatg gcctgtccga acaatgcgca gccaaaagcg attttggctc ggaaactgct
     1981 gcggaacagc cgcgccgttg gtagcggtgc ggaattcgcg gcggctcaga ttgttcagat
     2041 tagtcatgag ttgcgctttt ttagctccgg cagagacgtt ttcgcgcatc accaaacctg
     2101 cccatccgtc ggcaagcgga ttgatgcctg tcaccaaggc cgtaatgctg ccgtcaccgc
     2161 agagtgtgcg ttgtgcgaat gcctcggaat cggtggtgta attgggtccg taataacaat
     2221 tggtactggc gacggtccac acacctgtag cggtattgta atccacatcg ctgccgttgg
     2281 tacatccaat gccgttgacc ggagccgacc atccggcagg caggccggag acggttaaat
     2341 tggtagagca ggtggcggta ttaccctttt ggtcggtcac tgtaatgagt atcggcacga
     2401 tttggcccac ttgattggca ttgatgacac tgggggtgat tgtgatactt tgcactccgc
     2461 agttatcgtt ggccacgatc tgaaaatcaa cagggccgtt cattccgtca agttggatgc
     2521 tgttctgccc gttgaagttc aaggttgcat gactcgcgca ggtgatggta ggcggtgtgt
     2581 tgtcggtaca actgccaata ggagtgcctg ccgatgccgg gccggtcatc aaaggatcgg
     2641 agtcgcagga gcaacgcgtc ctaatgatat tgccggtaga aagattggca tacaggggca
     2701 gcgtgttgct ccattcgcca ttgttgacgg aatactgtat ggtgctgccc gcaggacaac
     2761 ctgaagtggg cgcagatatg ccggcgccta cgggttgaca ggccgcccca caggttccgt
     2821 tgttgatcaa cacctcgccc ggcgcagtac tcagattggg acaacagccc tcgtcaatgt
     2881 cgccgttgca gttgtcgtca atgccgttgc cgcagacttc cgttgcgccg ggatttatca
     2941 tcccacctgt ggcgggatcg tcgttgcaat ccgtgttgtc taacacataa cctgaaggcg
     3001 gagagtcggc caatatgctg ttgttcgggt cgccgaagcc atcgccgtcc atgtcgcgat
     3061 agtaggtgat gccggtttca tcggtcatgc cgttgcagtt atcatcaatg ccgttgacga
     3121 cctctgtggc gcctggatta acggcgttgt tgttgtcgtc gcagtctgtg ttgttggcta
     3181 catagcctat gggctggttg caggtgaagc ccggaataga gttattcgga tcgccataag
     3241 tatcggcgtc ggtatctgca taccaagtgg tgggaacgtc cgctacgccg cagccgcaag
     3301 cgccgggagc agtttttaac ggatcgttgg gacagccatc gttgcaatcg agcgtattgt
     3361 cgccatcggt gtcggtatcc gcaacgccgc agccacaagc gccgggagca gttttgttgg
     3421 gatcattggg gcagccgtcg ttgcaatcag gcgtat
//
LOCUS       DAORLF010000476         3428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15005_length_3428_cov_1.888527, whole genome shotgun sequence.
ACCESSION   DAORLF010000476 DAORLF010000000
VERSION     DAORLF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15005_length_3428_cov_1.888527"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>338
                     /locus_tag="PK198_24255"
     CDS             <1..>338
                     /locus_tag="PK198_24255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41937.1"
                     /translation="IKNVFHFSDGRITTLEKEYEYDDKDNPVVEYQIRDGIKRLVREM
                     KYNEYSQLVGVSFFYKEGLTEFLSIQKPGLLNEGDRVEQILTYYNNGLPESNSEYVNG
                     EIRLSIFYRY"
     assembly_gap    339..361
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(435..842)
                     /locus_tag="PK198_24260"
     CDS             complement(435..842)
                     /locus_tag="PK198_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41938.1"
                     /translation="MKRMLISGMVAGAILLALSYGVLFVTIRMFPAVVEEYYNPIFWP
                     GGERALLFFAHPFVLSICLAWFWDRSKSLYTGAWWFRGAEMGLVYGVVATLPSMWITF
                     SAISVSLAMVLTWLLYGFVQSVIAGLVFARLNP"
     assembly_gap    1006..1016
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1017..1519)
                     /locus_tag="PK198_24265"
     CDS             complement(<1017..1519)
                     /locus_tag="PK198_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41939.1"
                     /translation="MKKYHILALLSAVLLFGACEKDKESFLQLDGENSTGPLLEVGQH
                     ETAVRFSSSLTGRYNGKKLTAVRWYTGPRAVSTTVKVYGPGSNNGPGTVLYTKDVSSS
                     VRPFSWNEHVLDTPVSITGEELWLGIAFTHAETGQTIGCDAGPNKSGGDWLFTADGQW
                     RTYIQRTG"
     gene            complement(1646..2152)
                     /locus_tag="PK198_24270"
     CDS             complement(1646..2152)
                     /locus_tag="PK198_24270"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HRF41940.1"
                     /translation="MIYAFDTYYHSDRAKTVGIGFDRWDAGDCAGIWEDWTAVPGEYV
                     SGEFYKRELPCLLQLLTRIQPTAQDILVVDGFVVLDDTGKPGLGSRLHEALGGNVPVI
                     GVAKSNFAGLNLLKREVLRGDSARPLYITAAGLDLDLAADHIRQMKGEYRMPDLLKKL
                     DQLTRTNG"
     assembly_gap    2262..2271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2272..>3428)
                     /locus_tag="PK198_24275"
     CDS             complement(<2272..>3428)
                     /locus_tag="PK198_24275"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRF41941.1"
                     /translation="FSGPLDRIDAFIKGHVNDSTRMVIPISSDREAQAIQYVNFVNRK
                     AQAEADTRPSRPPTPKGMNVEMELTIGSEAVMQLIIDEQAGDVVEGKGRGNIRIQLPR
                     NADFQMFGEYLIEEGDYLFTLYNVVNKKFQVQRGGSIRWSGDPYKAIIRLDAEYKGIS
                     TPVAGFIADFIAGASAQVKTEASKSTQVNLILHLAGELFKPQISFDLEFPLLTGELKN
                     FTESKLRLLRQDPNELNRQAFGLVVAGQFLPDDLGIQADNAIFNTVSELLSNQLSLLL
                     TDLFSEMIADGRVLSGIDFDVAYSQYRPGDDLNRPGRGEEFEVRLRQNYFNDRLSVVV
                     GGNYDTGGSNVAATPGANNGAFLGNDVVIEYAISADRSLTLKVYQRLQPDI"
BASE COUNT          826 a          882 c          895 g          781 t
ORIGIN      
        1 cattaagaat gtctttcatt tttccgacgg acgaatcaca accctcgaaa aggagtatga
       61 gtacgacgat aaagacaatc cggttgtgga gtatcaaatc cgtgacggaa tcaaacgatt
      121 ggtcagagaa atgaaataca acgaatattc tcagttggtc ggggtctctt ttttttataa
      181 agaaggtctg acggaattcc tcagtattca aaaaccagga ttgttgaatg aaggtgatcg
      241 ggtagaacaa atcctcactt actacaacaa cggactgccg gagtcgaata gcgaatatgt
      301 gaacggcgaa ataaggctga gtatatttta tcggtatann nnnnnnnnnn nnnnnnnnnn
      361 ngcttttcaa catttgaatg ttgaaaagct ctgaagaata cctccccctc aacagcgaac
      421 cgacaaaata acgctcaggg gttcagcctc gcaaaaacca agcccgcaat cacagactgc
      481 acaaaaccgt agagcagcca ggtgagtacc atcgccagag ataccgaaat ggcgctgaag
      541 gtaatccaca tggacggcaa cgtggccact acgccataca ccaagcccat ttcggcccct
      601 ctgaaccacc aggctccggt ataaaggctt ttggagcgat cccaaaacca agccagacaa
      661 atactcaaca caaaagggtg agcaaaaaac aacagcgcgc gttcgccgcc gggccagaaa
      721 ataggattgt aatattcctc tactaccgcc ggaaacatac gaatggtgac gaacagtacc
      781 ccgtagctca aggccaacag gatagcgccc gccaccatgc ctgaaatcaa cattcttttc
      841 atactggttc tatttgatgg acgactgatg ccgtaaagct aatcatttag tagtggggca
      901 aaaataaata ttcatttttt tatcgcccct tagaaacaac gctaccaaac agaagccggg
      961 aaaagatcaa tcctccactc tgccgcgaat gttccagttc acgctnnnnn nnnnnnccgg
     1021 tgcgttggat gtaagtgcgc cattgcccgt cggcggtgaa gagccagtcg ccgccgcttt
     1081 tgttggggcc ggcatcgcag ccgatggttt gccctgtttc ggcgtgcgtg aaggcaatac
     1141 cgagccagag ttcttcgcca gtaatggaaa caggcgtatc gagcacatgc tcattccagg
     1201 agaaggggcg caccgaactg gaaacatctt tggtgtagag caccgtgccg gggccgttgt
     1261 tgctgcccgg accgtacact tttaccgtag tggaaacggc tcgcggaccg gtgtaccagc
     1321 gcacagcagt gagttttttg ccgttgtatc ggccggtcag cgaggaagag aaacgcacgg
     1381 ctgtttcgtg ctggccgact tcgagcagcg ggccggtgga gttttcgcca tccaattgga
     1441 ggaaactttc tttgtccttt tcgcaggcgc caaacagcaa tacggcactc aggagggcta
     1501 aaatgtggta ctttttcata ttttgaacag tttttttgca aaaataagcc atcaggaggg
     1561 gcgcaggtgt taaaacgaac ttaaagctgg ttaaggtgat gataatgtgc cgatgtatcc
     1621 tgtagagacc atttcaaccg gatgctcatc cgtttgttcg cgtcagttgg tccaattttt
     1681 taagcaaatc gggcattcga tactcgccct tcatctgtcg aatatgatcg gcggcaaggt
     1741 ccaaatccaa gccggcagca gtgatgtaca aaggccgggc gctgtcgccg cgcagtacct
     1801 cgcgttttag cagattcaaa ccggcgaaat tgcttttggc cacaccaata acgggcacgt
     1861 taccgcccag cgcttcatgc agccgactgc cgaggcccgg ttttccggta tcgtccaata
     1921 ccacaaaacc gtccaccaca agaatgtcct gggctgtggg ttgaatgcgc gtgagcagtt
     1981 gcaggaggca gggcaattcc cgtttgtaaa actcgccgct gacatattcg ccgggcacgg
     2041 ccgtccagtc ttcccaaatt ccggcacaat caccggcatc ccagcggtcg aatccgatgc
     2101 ccactgtttt ggcgcggtcg gaatggtaat aggtatcaaa agcgtagatc atcagccggg
     2161 cggtttgggc ttctttcctc cggccagctt gatgctgcgc agaaaatcgc cgaaagaatc
     2221 atactctttg cggaaactga ggcctgcgcc caccttgaac cnnnnnnnnn ngatgtcggg
     2281 ttgcaggcgc tgatacactt tgagcgtgag gcttcggtcg gcgctgatgg cgtactcaat
     2341 gaccacgtcg ttgcccaaaa acgcgccgtt gttggcgccg ggcgttgcgg ccacattgct
     2401 gccgccggta tcgtagttgc cgcccaccac caccgaaagg cggtcgttga agtagttttg
     2461 gcgcaggcgc acctcaaatt cctctcctct gccgggccgg ttgaggtcgt cgccggggcg
     2521 gtattggctg taagccacat cgaagtcaat gccggaaagg acgcgcccgt cggcaatcat
     2581 ctcagaaaat aaatcggtga gcaacaatga taactgattg gaaagcagct cgctcactgt
     2641 gttgaaaatg gcgttgtcgg cctgtatgcc caaatcatcg ggtagaaact ggccggctac
     2701 gacaaggccg aaagcctgcc ggttcagctc gttggggtct tgtctcagca gccgcagttt
     2761 gctctctgta aagtttttca gctctccggt gagcagcgga aattccagat caaaactgat
     2821 ttgtggtttg aataactcgc ccgcaaggtg caaaatcaga ttgacctgcg tggatttgga
     2881 ggcttcggtt ttcacttgcg cagaagcgcc ggcaataaaa tcggcgataa aacctgccac
     2941 cggcgtgctg atgcctttgt actccgcatc taagcgaatg atggctttgt agggatcgcc
     3001 cgaccaacgg atggagccgc ctctttgcac ctgaaacttc ttgttcacca cattgtacag
     3061 cgtaaagagg tagtcgccct cttcgatgag atactctcca aacatctgaa aatcagcgtt
     3121 tctggggagc tgtatgcgaa tatttccccg gcctttgccc tccaccacgt cgccggcttg
     3181 ctcgtcaata atgagttgca tcacggcctc gctgccgatg gtgagttcca tttccacgtt
     3241 catacctttg ggcgtgggcg gtcgcgaagg cctcgtgtcg gcctctgcct gggctttccg
     3301 gtttacgaaa ttgacgtatt gaatggcctg tgcttcgcgg tcggaactga tgggtatgac
     3361 catgcgggtg ctgtcgttca catggccctt gatgaaggcg tctatgcggt cgagcgggcc
     3421 gctgaaac
//
LOCUS       DAORLF010000477         3424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15030_length_3424_cov_1.622440, whole genome shotgun sequence.
ACCESSION   DAORLF010000477 DAORLF010000000
VERSION     DAORLF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_3424_cov_1.622440"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2351
                     /locus_tag="PK198_24280"
     CDS             171..2351
                     /locus_tag="PK198_24280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF41942.1"
                     /translation="MLGRTITFLVALVMFSATSWAQTAYKTVTKTSADGKYTWQEVEN
                     DPTRSRFYTLKNGLTVILAENKLEPRIMTLFATKAGSKNDPATNTGLAHYLEHMLFKG
                     TDRFGSLDYAKEKVELDRIEALYERYNKTIDAEARKAIYRQIDSVSSVAAKYAIANEY
                     DKMMSALGSNMTNAFTSFENTTYMENIPSNNLERFLKVQDERFRNPVLRLFHTELEAV
                     YEEKNISLDDGSNKVFEAMFASLFKKHPYGTQTTIGTIEHLKNPSLKAIRDYYNAYYV
                     PNNMALILAGDLDPDQTIALVDKYFGDWQNKAVPPFKFEPEAPRNTVEEITVTSPDEE
                     SVAIGYVMPSALSKEAVVADLVSSILYNGKSGLVDKNLVKQQKVLEAYGFNYLLKDYG
                     IFYFGGRPLEGQSLQELRDLILMEVENLKKGNFEEDLIAATVNNLKVSRIQEQESAMN
                     MAFVLHDQFVTGKPWADYMAGIDAMSRITKQDVVNFANKWFGNNYTVVYKLSGEDESA
                     QKVEKPSINPVEVNRESQSDFLRSIVETPAQPLSPVFLDYNKDIQKTQLKKTIPVWGV
                     KNNINNLFSLYYVLDMGENHNKKLPLAVNYLQLIGTSKMSNEQINKEFYKLAVDFSIF
                     ASRDQVYVSLSGLQENLVPAVKLLESLLNDPKPDQDALDKMIAAKIKERNDATLNKRA
                     IFWQGLDAYVGYGPVNPFNDVLSNADMRAIKAAELTDIIKSLPK"
     gene            2394..>3050
                     /locus_tag="PK198_24285"
     CDS             2394..>3050
                     /locus_tag="PK198_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF41943.1"
                     /translation="MAAQLKTLHKTPAKLNDYPAEKQYQRQMQAGNTIYFVDYDMVQA
                     EVAMQRWDESFNAKLLPMVSAFNEYYGGGMGSVVFQDIRESKALAYSAFSSFARPGKK
                     EDPFSALFYIGTQADKLPDAMAAMKNLMTNMPESAKLWEVGQRSIKQGIETRRITKTG
                     ILFNYQSALRLGLDYDTRRDIYNAVDKITLNDIKQFHADRFAGKNWTVRLIGSKKRLD
                     M"
     assembly_gap    3051..3157
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          894 a          940 c          771 g          712 t
ORIGIN      
        1 gttttgttat gccagatgca ggccgtaagc tgaaagggtt tacggcctgt gttgttttag
       61 gggcacattt cgaccagcgc ccgcttttct tcgccaaaag atggaataag aaccgcactt
      121 cggcgttgtt gcagttgttt taactttctt tatctcacaa ctcttcaatc atgcttggaa
      181 gaaccattac attccttgtt gcgctggtta tgttcagtgc aacatcctgg gcgcaaaccg
      241 cctacaaaac cgtcaccaaa acttctgccg acggcaaata cacctggcag gaagtggaaa
      301 acgaccctac ccgctctcgt ttttacacgc tcaaaaacgg cctcaccgtc attttggctg
      361 aaaacaaatt ggagccgcgc atcatgactt tgtttgccac caaagccggc agcaaaaacg
      421 acccggccac caacacgggc ctggcacact acctcgaaca catgcttttt aagggtaccg
      481 accgtttcgg ctctttagat tatgccaaag aaaaagtaga actcgaccgc atcgaagcgc
      541 tgtacgaacg ctacaacaaa accattgacg cggaggcgcg caaggccatt tatcgccaga
      601 ttgactctgt ttcaagcgtg gccgccaagt acgctattgc caacgaatac gacaaaatga
      661 tgtcggcgct ggggtcaaac atgaccaatg ctttcacttc gtttgagaac acgacctaca
      721 tggaaaacat cccctccaac aatttggagc gttttctgaa agtgcaggac gagcgtttcc
      781 gcaatccggt gctgcgcttg ttccacaccg agttggaagc tgtttatgaa gaaaaaaaca
      841 tcagcttgga tgacggcagc aacaaggttt ttgaggccat gtttgccagc cttttcaaaa
      901 aacaccccta cggtacccaa accaccatcg gcaccataga gcacctcaaa aacccctcat
      961 tgaaggccat ccgcgactac tacaatgcct actatgtgcc caacaacatg gccctcattc
     1021 tggccggcga tttagacccc gatcagacca ttgctttagt ggacaagtac ttcggcgact
     1081 ggcagaacaa agccgtgcct ccgttcaaat ttgagccgga agcgccccgc aacaccgtag
     1141 aggaaatcac cgtgacaagc cccgacgaag aaagcgtggc cattgggtat gtaatgccca
     1201 gcgcgctctc gaaggaagcg gtggtggctg atttggtcag ttccattctg tacaacggca
     1261 agagcggctt ggtggacaaa aacttggtga agcagcaaaa agtactggaa gcttatgggt
     1321 tcaattacct cctcaaagac tacggcattt tttacttcgg cggtcgtcct ttggagggcc
     1381 agagcttgca ggaactccgc gacctcatcc tgatggaagt ggaaaatctg aaaaagggca
     1441 attttgagga agacctcatc gccgctaccg tcaacaacct gaaagtgagc cgcatacaag
     1501 agcaggagag cgctatgaac atggcctttg tgctgcacga tcagttcgtt accggcaagc
     1561 cctgggccga ttatatggca ggcatagacg ccatgagccg catcaccaag caagacgtgg
     1621 tgaattttgc caacaaatgg ttcggcaaca actacacggt ggtttataaa ctctccggcg
     1681 aagacgaatc cgcccaaaaa gtagaaaaac cctccatcaa cccggtagag gtgaaccgcg
     1741 aatcgcagtc cgacttcctg cgctccatcg tggagacgcc cgcacagccg ctttcgcctg
     1801 tttttctcga ttacaacaaa gacatccaga aaacacagtt gaaaaagacg attccggttt
     1861 ggggtgttaa aaacaacatc aacaacctct tttcgctgta ttatgtattg gacatgggtg
     1921 agaatcacaa caaaaaactg cccttggccg tcaactacct ccagctcatc ggcacttcca
     1981 aaatgagcaa tgagcagatc aacaaggagt tttacaagct cgccgttgac ttcagtatat
     2041 ttgccagccg cgaccaagtg tatgtctccc tctccggcct tcaggaaaac ctcgttcccg
     2101 ctgtgaagct cctcgaatcg ctgctcaacg accccaagcc cgatcaggat gctttggaca
     2161 aaatgattgc cgccaaaatc aaagagcgca acgacgccac cctcaataaa cgcgccattt
     2221 tctggcaggg cctcgacgcc tatgtgggtt acggccccgt caacccgttc aacgacgtgc
     2281 tcagcaacgc cgacatgcgc gccatcaaag ccgccgaact gaccgacatc atcaagtcgc
     2341 tgcccaagta gaagcaccgc gtttattact acggtcccaa aacggtgacg gaaatggccg
     2401 cccaactcaa gacgctgcac aaaacgccgg ccaaactcaa cgactacccg gccgaaaagc
     2461 aatatcagcg ccagatgcag gcaggaaata ccatctattt tgtggactac gacatggtgc
     2521 aggccgaagt ggccatgcag cgctgggacg agtcgttcaa cgccaaactg ttgcctatgg
     2581 tgagcgcttt caacgagtac tacggcggcg gcatgggttc ggtggtcttc caggacatcc
     2641 gcgaatctaa ggcgctggcg tactctgcct tctccagctt tgcacgtccc ggcaagaagg
     2701 aagacccctt cagcgcgctg ttttacattg gcacacaagc agacaaactg cccgacgcca
     2761 tggctgccat gaaaaacctg atgaccaata tgcccgaatc ggccaagctg tgggaagtgg
     2821 ggcagcgttc catcaagcag ggcattgaaa cccgccgcat caccaaaacc ggtatcctgt
     2881 tcaactacca aagcgccctg cgcctcggcc tcgactacga tacccgccgc gacatctaca
     2941 acgctgtgga taagatcacg ctgaacgaca tcaagcagtt tcacgcagat cgcttcgccg
     3001 gcaaaaactg gacggtgcgc ctcattggtt ccaaaaagcg ccttgatatg nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa aatagagcgg gggtaagata
     3181 ctttgtttga tatccatgtt ttacctttgt ctctataaag cctccaactg aaaatgaggc
     3241 ggttgcttga acaaagcgcc gcctcacttt ttgaacccgt ttcttcataa ccaaaacctt
     3301 attcatcatg caaaacctcc aatttccgct cgaatttcag ttcaaaatcg cgacgctgtc
     3361 caacgatttt accgccacag acgcttccgg cagaaccctg gcttatgtgc gtcagaagct
     3421 cctc
//
LOCUS       DAORLF010000478         3414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15106_length_3414_cov_2.247990, whole genome shotgun sequence.
ACCESSION   DAORLF010000478 DAORLF010000000
VERSION     DAORLF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15106_length_3414_cov_2.247990"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1361)
                     /locus_tag="PK198_24290"
     CDS             complement(<1..>1361)
                     /locus_tag="PK198_24290"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF41944.1"
                     /translation="FWTDLPNTPKKFNRIDDLEEKIGPLETILLNKDAYLHFASGDGA
                     CLGCSEKTVMHLFVATVESLMQPRIQKHLAYLDDLIEKMQAHIQRKLMQNVDLGDSET
                     MAKIVSEAKNSDLTMAGLTSKFESARGGEPIDQDWLREVTQLLAKLKQLKWKYTNGTT
                     GNGRSSMGICNSTGCTSVWGSTFPYNPYPFPWTNHLFQDSTSLAMGIFEGHMSKMAEG
                     FAAIRKAELELKGEYDAAKNREFFTFFNWHQFTEEEWHLCPPVVAVGGDGSMYDIGFQ
                     NLSRMMASGKPIKVLIVDTQVYSNTGGQACTSGFIGQVSDMAQYGKAIQGKSEPRKEI
                     GLIAMAHRNTYVLQGTLANTSQMIEGFIDGLMTKRPALFNLYTTCQPEHGVADDLGTH
                     QAKLAMESRAYPIFKYNPDKGVKAEECFDLSGNPAMDRLWPTYTLKYMENGREKTMDV
                     DMT"
     assembly_gap    1362..1401
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1402..>1656)
                     /locus_tag="PK198_24295"
     CDS             complement(<1402..>1656)
                     /locus_tag="PK198_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF41945.1"
                     /translation="YEAMEDSIREANGDGAALAREFEWFREELGEFKFALTRPYFDVF
                     EKKQANSGGLFSITINPTTCKGCMECVEVCHDDALKIVKQT"
     assembly_gap    1657..1830
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1831..>3414)
                     /locus_tag="PK198_24300"
     CDS             complement(<1831..>3414)
                     /locus_tag="PK198_24300"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRF41946.1"
                     /translation="QAFEEYYQLTGRRYARVMSYRAEDADYLILGQGSIVPSTEAVVD
                     YLRKTRGIKVGVVDLVMFRPFPSDLIAKVLKGRKGVTILERLDQPLAVDPPIAREVRA
                     TLTKCLENGAAAKGRIPYPELESYRATDMPAIYTGSFGMGSRDLQPEGIIGAIENMLP
                     EGKQKKMFYLSIDFIRDVAHTPKQRAYQESLQEAYPHVKELSVRGSENPNLMPEGAIT
                     VRFHSVGGWGAITTGKNLAMTLFDLLGYDIKANPKYGSEKKGQPTTYYLAAAPEPIRM
                     NCEFFFVDVVLSPDPNVLKHTNALAGLKKGGVFIIQSERQTAAEVWADIPKPYQKVII
                     DNNIRLYYIDGFKIAREEATDPELQLRMQGIAFQGAFFASSPLMQKAGLSEESLLKAI
                     EDQLEHKFGSKGRRVVDDNMRVVRRGFDEVYEVTEKVLGAGHEAKTNGEVSQPVPAML
                     KSMPKSESNLSDIHRFWEQTGNFYQRGMGNDNITDPFIGLSIMPAVTSVFRDMSGIRF
                     EHPEWVPNNCTACGNCYTAC"
BASE COUNT          604 a          883 c          932 g          781 t
ORIGIN      
        1 ggtcatgtcc acgtccatcg ttttttcacg gccgttttcc atgtacttga gtgtgtaggt
       61 cggccacagg cggtccatag cggggttgcc gctcagatcg aaacattcct ccgccttcac
      121 gcccttgtcc gggttgtatt tgaagatcgg ataagcgcgg gattccatcg ccaatttggc
      181 ctggtgcgtg cccagatcgt cggccacgcc gtgttcgggc tggcaggtag tgtagaggtt
      241 gaacagggcc ggacgtttgg tcatcagacc gtcaatgaag ccctcgatca tctggctggt
      301 gttggccagc gtgccttgca acacataagt attgcggtgc gccatggcga tgaggccgat
      361 ttccttgcgc ggctccgact tgccctgaat ggccttgccg tactgcgcca tgtcggacac
      421 ctgcccgatg aagccggagg tacatgcctg gccgccggtg ttggaataca cctgcgtgtc
      481 cacaatgagc actttgatcg gcttgcccga tgccatcatg cgcgaaagat tctggaaacc
      541 aatgtcgtac atcgagccgt cgccacccac ggccactacc ggcggacaca gatgccactc
      601 ttcttcggta aactgatgcc agttgaagaa ggtgaaaaat tccctgtttt tggccgcatc
      661 gtactcgcct ttcagttcga gttctgcctt gcggatggct gcaaaaccct cggccatttt
      721 cgacatgtgc ccctcaaaga tgcccattgc cagcgaggtg gaatcctgga acaagtggtt
      781 cgtccagggg aatgggtagg ggttgtacgg gaaggtgctg ccccacacgg aggtacagcc
      841 ggtgctgttg caaataccca tcgaactgcg gccgttgccg gtggtgccgt tggtgtattt
      901 ccacttcagt tgcttcagtt tggccagcaa ttgcgtcact tcgcgcagcc agtcctgatc
      961 tatgggttcg ccgcctctgg ccgattcaaa tttgctggtg aggccggcca tagtcaggtc
     1021 gctgttctta gcttcagata cgattttggc catcgtttca gaatcgccta agtccacatt
     1081 ttgcatcagc ttccgctgga tatgggcctg cattttttca atcagatcgt ccaaataagc
     1141 caagtgcttc tggatgcgcg gctgcatgag cgattctacc gttgccacga agaggtgcat
     1201 gacggttttt tcagaacaac cgaggcaggc gccgtcgccg ctggcaaagt gcaggtacgc
     1261 gtctttgttc agcaggatgg tttccaaagg cccgattttt tcttccagat cgtcaatgcg
     1321 gttgaacttc ttcggcgtgt tgggcagatc ggtccagaag tnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn ngtttgtttg acgattttca gcgcgtcgtc gtggcatact
     1441 tccacacatt ccatacagcc tttgcaggtc gtggggttga tggtgatgct gaacaggccg
     1501 ccgctgttgg cttgtttctt ttcaaaaaca tcgaaatacg ggcgggtgag ggcaaattta
     1561 aactcgccca attcctcgcg gaaccactcg aactcgcgtg ccagcgccgc gccgtctccg
     1621 ttggcctccc ggatggaatc ttccatcgct tcgtaannnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaggcggtg tagcagttgc cgcaagccgt
     1861 gcagttattg ggaacccact ccggatgctc gaaacgaatg cccgacatat cgcggaaaac
     1921 ggaggtaacg gcaggcatta tgctcaaccc gatgaagggg tcggtgatgt tgtcgttgcc
     1981 catgccgcgc tggtagaaat tgccggtttg ctcccagaaa cggtggatgt cgctcaggtt
     2041 cgattcgctc ttcggcatgc tcttcagcat ggcaggtacg ggctggctga cctcgccgtt
     2101 ggttttggct tcgtggccgg cgccgagtac cttttccgtc acttcataca cctcgtcgaa
     2161 gccgcgacgc accacgcgca tgttgtcgtc caccacgcgg cggcctttgg aaccaaactt
     2221 gtgttccaac tggtcttcga tggctttcag caggctctct tccgacaggc cggctttttg
     2281 catcagcggc gaagaagcga aaaatgcgcc ctgaaacgcg atgccctgca tgcgcagttg
     2341 cagttcgggg tcggtggctt cctcgcgggc aattttgaaa ccgtctatgt aatacaggcg
     2401 gatgttgttg tcaatgatga ctttctggta gggtttggga atatcggccc agacctccgc
     2461 cgctgtttgg cgttcgcttt ggatgatgaa cacgccgcct tttttcaaac cggccagcgc
     2521 gttggtgtgc ttcagtacgt tggggtcggg cgagagcacc acgtccacaa agaagaactc
     2581 gcagttcatg cggatgggtt cgggggccgc cgccagatag taggtggtcg gctggccttt
     2641 tttctcggag ccatacttgg gattggcctt gatgtcgtag cccaacaggt cgaacagcgt
     2701 catggccaag ttcttgcccg tagtgatggc gccccagccg cctaccgagt ggaaacgcac
     2761 cgtgatggcg ccctccggca tgaggttggg attttcagaa ccccgcaccg acagctcttt
     2821 cacatgcgga tatgcctcct gcaaagactc ctgataagcg cgctgtttgg gcgtgtgcgc
     2881 cacgtcgcgg atgaagtcaa tggagaggta aaacattttt ttctgcttgc cttccggcaa
     2941 catattctct atggcgccga tgatgccctc cggctgcaag tcgcggctgc ccatgccgaa
     3001 agaaccggtg taaatagccg gcatatcggt agcgcggtag ctttccaatt cgggatacgg
     3061 gatgcgacct ttggcggcag caccgttttc gaggcatttg gtgagggtgg cccgcacttc
     3121 tctggcgatg ggcgggtcaa cggccagcgg ctggtccaag cgctccagaa tggtgacgcc
     3181 ttttctacct ttcagcacct tggcgatgag gtcggagggg aaaggccgga acatgaccaa
     3241 atccaccacg cctactttaa tgccgcgggt tttacgcagg tagtccacca cggcttcggt
     3301 gctgggtacg atgctgccct gccccagaat cagataatcg gcgtcctctg cgcgatagct
     3361 catcacgcgg gcatagcggc gcccggtgag ctggtagtat tcttcaaaag cctg
//
LOCUS       DAORLF010000479         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15193_length_3401_cov_1.944710, whole genome shotgun sequence.
ACCESSION   DAORLF010000479 DAORLF010000000
VERSION     DAORLF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15193_length_3401_cov_1.944710"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>362
                     /locus_tag="PK198_24305"
     CDS             <1..>362
                     /locus_tag="PK198_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41947.1"
                     /translation="VEEADSITLYTLRLQNAAGGHAVAIGEARQAELLIEAGIRCRVQ
                     QDSAAVNCALTDTTSIHNGVPLISTLKKDDSFWAGDFEVKVTQANGNGTFNGKGIITV
                     PYLQQAQINVILKDVRVN"
     assembly_gap    363..466
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <467..>2464
                     /locus_tag="PK198_24310"
     CDS             <467..>2464
                     /locus_tag="PK198_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41948.1"
                     /translation="TLDGIDNVLGHLQNAMNVISTVVNSSSAVSSVLGSNWNVLQGAG
                     PIYNEYPYLPDSLAQHINNAVLCYLTNPPDVCNQVLAGNIQQIIQAIQDLFEADYAVR
                     FLPYSPTTFGFDTLAYQAMHEKYNFFQIAGERYFAPWASVAVQAGDTVTINIPHDQLP
                     EDLSFEDNRRNPVEMIVQDNKVLLPIRGAAEEGVHQLYAVQLKTDSSGNPVKKYAGKL
                     NILTYPRKTIRVELVPVNQSVVVTDSLRSRARRALQVVYEPAVVQIEVSAGEHLSLPS
                     LGESLDTTGTGIFAAYNPEMRALTEAVKNRSGFSNETYYIMLVTQFDDAAQLGFMPKG
                     RKFGFVALQPHSDEAQLIRTIAHELGHGTAVLNHTWEDFTSLPSGQTDNLMDYGSGTR
                     LHKYQWERIQHPPLHLAQLDEEEEGAGFVISKYFENAALHYEHSDAAPDAKKTYFTPA
                     GDLFTLPARAVCSFLKSASNASRWPAGCLIGFVIDGKVYSAYWYKNTEKFTGYKNNVN
                     EPYYPNLTPDATNNVVEVHAAKLDACTAQLLRGTFSGFDVWTQSNEGKSISTGLPLDQ
                     MDAIATSIWYSPLCSHQEWLAQNEACRLHYQGHPYLAPGANDPVSRYVKANPCVLNSL
                     GWYDFKAPLPFGDSTYQQLGDQVGFALGLLTVGIVAAPVVLA"
     assembly_gap    2465..2488
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2669..>3401
                     /locus_tag="PK198_24315"
     CDS             2669..>3401
                     /locus_tag="PK198_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41949.1"
                     /translation="MLTAAEAAIHFNGYESEIAAISNCVAGGFWNESGQLRDEFSWQS
                     CFYSSAAAVITHGILNVDPHKLPLFKKFPTALARLKALPKPALVRGYRRLLTQFPSAA
                     GSLDLWALHRFLKTNLNKDDIAALFDLPANAPELSAIAQNLNFPHNAQVHTVLGEGNW
                     WYQLSKTFPDNPLKRAQLLQDISGNANFAGALKARPELVGAWERMISAPTSRVDIPIL
                     SSVSIAINRGIKGCNSKCRIRQPLLI"
BASE COUNT          824 a          914 c          792 g          743 t
ORIGIN      
        1 cgtggaggag gccgacagca tcaccctata caccctgcgc ctgcaaaacg cagccggcgg
       61 acacgccgtc gccatcggcg aagcccgaca ggctgaactc ctcatcgagg ccggcatccg
      121 ctgccgcgta caacaggact ctgccgccgt caactgtgcc ctcaccgata ccacctccat
      181 ccacaacggc gtgcccctca tcagcaccct caaaaaggac gacagcttct gggcagggga
      241 ttttgaggtg aaggtgaccc aggccaacgg caacggcact ttcaacggca agggcatcat
      301 cactgtgccg tacctccaac aagcgcagat caatgtcatt ctcaaagacg tgcgcgtcaa
      361 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct tggacgggat
      481 tgacaacgtg ctggggcatc tgcaaaacgc catgaacgtc attagcacgg tcgtcaacag
      541 ttccagcgcg gtttccagcg tgctgggcag caattggaat gttttacaag gcgcaggccc
      601 gatttataac gagtaccctt atctgcccga ttcactggcg cagcacatca acaacgccgt
      661 cctctgctac ctgaccaacc cgcccgacgt ctgcaatcag gtgcttgcag gcaacattca
      721 acaaatcatt caggccattc aggacttgtt tgaggcagat tacgccgtcc gttttttgcc
      781 atacagcccc accactttcg ggttcgatac gctggcgtat caggccatgc acgaaaaata
      841 caacttcttt caaatcgccg gagagcgcta tttcgcgccc tgggcttccg tagcggtgca
      901 ggccggagat acggtgacca tcaacattcc gcacgatcag cttcctgaag acctcagctt
      961 tgaggacaac cgaagaaatc cggtggaaat gatcgtacag gacaacaaag tgttgctgcc
     1021 cattcgcggc gctgccgagg aaggcgttca tcaattgtat gccgtgcaac tcaaaaccga
     1081 ttcaagtggg aatcctgtca aaaaatacgc cgggaaactc aatatcctga cttacccccg
     1141 caaaaccatt cgcgtagaat tggtgcctgt caatcagtct gttgtagtga ccgacagcct
     1201 cagaagccgc gcccggagag ctttgcaggt cgtttatgaa cccgccgttg ttcaaataga
     1261 agtcagcgcc ggcgagcatt tgtcgttgcc ttccctcggc gaaagccttg atactacggg
     1321 aaccggtatc tttgcagcct acaacccgga gatgagagcg ctcacagagg ccgtcaaaaa
     1381 tcgcagcgga ttcagcaacg agacctatta tatcatgctg gtgacgcaat ttgacgatgc
     1441 ggcacagctt ggctttatgc ccaagggacg caaattcggc ttcgtggccc tgcaacccca
     1501 ctccgacgaa gcgcaactca tccgcaccat agcgcacgaa ctggggcacg gcacggcggt
     1561 tctcaaccat acctgggagg actttacgag cctcccaagc ggccaaaccg acaatctgat
     1621 ggattatgga agcggtaccc gcctgcacaa ataccagtgg gaacgcatcc agcatccgcc
     1681 cctgcacctg gcgcaattgg atgaggagga agagggagcc gggtttgtta tcagcaaata
     1741 ttttgaaaat gctgcccttc attacgaaca ctctgatgcc gccccggatg ctaaaaaaac
     1801 ctatttcact cctgccggtg atttattcac cttgcctgca agagcagtct gttctttttt
     1861 aaagtcggcc tcgaatgctt cgagatggcc cgccggatgc ttgataggat ttgttattga
     1921 cggcaaggtt tattccgcat attggtataa gaacaccgaa aaatttaccg ggtacaaaaa
     1981 caatgtaaat gagccatact acccaaatct tacgcccgat gcaacaaaca acgtagtaga
     2041 agtacatgcc gcaaaacttg acgcttgcac agctcaatta ttaagaggaa cattttccgg
     2101 tttcgatgtc tggacacaaa gcaacgaagg aaagtcaata tcgactggac tgcccttaga
     2161 tcaaatggat gcaattgcaa catcaatttg gtatagccct ttatgctctc atcaagaatg
     2221 gctggcgcag aatgaagctt gccggctaca ttatcaaggt catccatacc ttgcaccggg
     2281 agccaacgac cctgtatccc gctatgttaa agccaatcct tgcgtcctca actcattggg
     2341 atggtatgat ttcaaggcgc ctctgccgtt tggcgattca acctatcagc aattaggaga
     2401 tcaagttgga tttgcattgg gcttgttaac tgtcggtata gtggctgccc ctgttgttct
     2461 ggcannnnnn nnnnnnnnnn nnnnnnnngg agttccggct tcaacggctg ctgttaccac
     2521 ttttgaattg cgccggcgtg ctgtagatgc ctctacggcc tgggctttgg acgtcgctgt
     2581 tcaggcttcg ctgaattatt tctggccttc gccggaggaa gaaatcgaca gaatagaagc
     2641 atttagaaga gtacattggc catcgtctat gctgactgct gccgaagcgg ctatccattt
     2701 caatggatac gaaagtgaaa tcgccgcgat cagcaactgt gtagccggcg gcttttggaa
     2761 tgaaagcggc caattaagag atgaattttc atggcaatcc tgtttctatt catctgcggc
     2821 ggcggtcatc acgcatggca ttctcaatgt tgacccgcat aaactaccgt tattcaaaaa
     2881 attccccacc gcccttgccc gccttaaagc cctgcccaag ccggcactgg tcagaggtta
     2941 ccgcaggctc cttacccaat tcccctctgc tgccggctct ttggacttgt gggcactgca
     3001 tcgctttctg aaaaccaacc tcaacaaaga cgacattgcc gccttgttcg acctgccggc
     3061 caatgctccc gaactaagcg ccatagccca aaacctgaac ttcccccaca acgcgcaggt
     3121 acacaccgta ctcggtgaag gcaactggtg gtaccaactc tccaaaactt ttcccgataa
     3181 tcccctaaaa cgcgctcaat tgttgcagga tatttcggga aatgctaatt ttgcaggagc
     3241 tttgaaagca aggccggagt tggttggggc gtgggaaagg atgatcagcg ctcctacatc
     3301 acgggttgac attcctattt tgagttcagt gtccatagct atcaatcgtg gaataaaagg
     3361 gtgtaattca aaatgtcgca tcaggcagcc ccttttaata g
//
LOCUS       DAORLF010000480         3373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15366_length_3373_cov_2.133213, whole genome shotgun sequence.
ACCESSION   DAORLF010000480 DAORLF010000000
VERSION     DAORLF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15366_length_3373_cov_2.133213"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK198_24320"
     CDS             complement(<1..201)
                     /locus_tag="PK198_24320"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF41950.1"
                     /translation="MAKKQKITPFLWYDHQAEEAAGFYTSVFKKSKILEVVRHTGGVQ
                     IVRFSLMGYEINALNGGPAFRFN"
     assembly_gap    249..296
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<297..606)
                     /locus_tag="PK198_24325"
     CDS             complement(<297..606)
                     /locus_tag="PK198_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF41951.1"
                     /translation="MDAYIAGFPVEVQVVLQQVRAAIRNIVPDAEETIRYAIPTFRYK
                     GRNLVHFAGFKNHIGLYATPSGHTAFEAELSKYKQGKGSVQFPLDQPMPLDLIERIVR
                     F"
     assembly_gap    623..659
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(733..1173)
                     /locus_tag="PK198_24330"
     CDS             complement(733..1173)
                     /locus_tag="PK198_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF41952.1"
                     /translation="MANVSIYLNFPRHTEAAFLYYQSIFGGEFDGGISRFGDMPPQEG
                     MPPMPETDRNLVMHVSLPILGGAFMLMGSDAPESMGFEVKIGNNVYINLSPDTREETR
                     RLFGALSDGGVVEMELQDTFWGAYYGSCTDRFGIRWMFNCEEKQ"
     gene            complement(1582..>3373)
                     /locus_tag="PK198_24335"
     CDS             complement(1582..>3373)
                     /locus_tag="PK198_24335"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF41953.1"
                     /translation="AEPALVALPPVAVDEVVDRPAVDRTERHAVGPADRDGGGDADGV
                     VTLSVQEGWAPYQYIWQQVGGSASGNGTIGAEGQAVTIPGLRAGDYRITVSDVFAQAT
                     VIATVSEPPALAIAVLQADSASCFSFCDGMMNVAVTGGTGAYLYAWSGPQAAGPQISG
                     LCAGSYRLSVTDANNCRTSQVIEIGEPAEFIAAASVLQSVTCFGGNDGRATVSTNGNP
                     IGYAWSNGDAAQEAMQLATGFYDVTVTNADGCEDVSSIFVQQPAAPVTVSITEDRPVT
                     CFGDSDGVLAAQPMGPGTVFSYRWSNGAAARENTGLNAGLYSVTVSNELGCEGSGSYA
                     LAQPDLITASLEIEHVNCRNIAQGGAIFIENVRGGTGAYSYSKDGAGFGSAPLFTGLL
                     PGSYDVRIRDERGCTYPFPATVDAPPVLTVSLGEDLLLPLGDSVLLSAETNSPAPVFS
                     WTSSEGDLPGTAQELSVRPLVGAAYTVTVFDTLSFCSVSDVVWIMVDKTRRVFVPNAF
                     SPNEDGYNDRFLVFSGSDVSIVRSFSVFDRSGGMVFRQTDLSPNDPAAGWDGTVRGRH
                     LNPGIFVWVAEIEFVDGEVEVFRGDVMVLR"
BASE COUNT          723 a         1029 c          822 g          714 t
ORIGIN      
        1 attgaaacgg aacgcagggc ctccgttgag ggcgttgatt tcatatccca tcaaagaaaa
       61 acgaacgatt tggacgccgc cggtatgcct gacgacttcc aaaattttcg attttttgaa
      121 caccgaagta taaaacccgg cggcctcctc tgcctgatga tcgtaccaga gaaacggcgt
      181 tattttctgc tttttggcca tggtgaggga tgcttaggtg gatgaaatgt tctgagtgcg
      241 cggctcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa
      301 acgcacgatg cgctcaatca aatccaaggg catcggttgg tcgaggggga attgcacgga
      361 gcctttgccc tgcttgtatt tcgatagttc cgcctcgaaa gccgtatgcc cgctcggtgt
      421 ggcgtacaat ccgatgtggt ttttaaaacc cgcaaaatgc accagatttc gccccttgta
      481 cctgaaagta ggaatggcat accggatggt ttcttcggca tcgggcacga tgtttcggat
      541 ggcggctctt acttgctgta acacgacctg cacttctacc ggaaacccgg caatgtaggc
      601 gtctattgta ctgacattct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      661 ctacaaactt ctaccccgcc aagagcttga ggcttgccgc ttacggcctg tagccgttcc
      721 taccccgtcc ccctactgtt tctcctcgca attaaacatc cagcgaatcc cgaaccggtc
      781 ggtgcaactg ccataataag cgccccaaaa cgtatcctgc aactccattt ccactacgcc
      841 gccatccgac agcgcgccaa acaagcggcg ggtttcctcg cgggtgtccg gtgagagatt
      901 gatatataca ttattgccga tttttacttc aaagcccata gactccggcg catcagaccc
      961 catcaacata aatgcaccgc ccaatatcgg caaggataca tgcatgacaa ggttccggtc
     1021 ggtctccggc atgggcggca tcccttcctg cggcggcata tctccgaaac ggctgattcc
     1081 tccatcaaac tcgcctccga aaatggattg gtaatacaaa aaagcggctt ctgtgtgccg
     1141 ggggaaattg aggtaaatgc tgacgttggc catgattttg gatgtttttt gttaaaaatt
     1201 gttttaaaat cttcgcaaaa ctaagtcttt gttttcaagg gccaaacatt ttagttgatt
     1261 ttttaaattt tttgtgttaa aattaaaata ctcaacccta ctactccaca tcgctaccat
     1321 cgggtatatg tcattgaaaa caatacgact gcttccgcca tcgggctgat gtgcatgttg
     1381 acggtaaact tggctatctt tttcatccat cctatattta aggcttaaag attgcaaccg
     1441 ttccatcccg ccaagagctt gcagcctgca gcttacggcc tgcagccgtt cttaccttgt
     1501 tcccagccac cagccgttcc atcccgccaa gagcttgcag cctgcagctt acggcctgca
     1561 gccccgctcc taccccgttc cctatctcag caccatcaca tccccccgaa acacctccac
     1621 ctccccatcc acaaattcaa tctccgccac ccacacaaaa attccgggat tcaaatggcg
     1681 gccccgtact gtgccgtccc agcctgccgc cggatcattg ggcgacaagt ccgtttgccg
     1741 gaacaccatg cccccgctgc ggtcaaacac gctgaaactg cgcactatgc tcacatccga
     1801 gccgctgaaa accagaaagc ggtcgttgta gccgtcctca ttgggcgaaa aagcattggg
     1861 gacaaatacc cgccgcgtct tgtccaccat gatccacacc acatccgaaa cactgcaaaa
     1921 cgagagcgta tcaaaaaccg tcaccgtata agccgcgccc accaatggcc gcacgctcag
     1981 ttcttgcgcc gtgcccggca aatcgccctc ggaggaggtc caactgaaca ccggggccgg
     2041 actgttggtc tccgcgctca gcaacaccga atcgcccaga ggcaataaga ggtcttcgcc
     2101 cagcgatacc gtgagtaccg gcggagcgtc taccgtggcc ggaaaaggat aagtgcagcc
     2161 gcgctcatcg cgtatgcgca catcgtagct gcccggcaac aggcctgtaa acaaaggcgc
     2221 cgagccaaaa cccgcgccgt ctttcgagta gctgtacgcg ccggtgccgc cccgcacatt
     2281 ttcaataaaa atagcgccgc cctgcgcgat gttgcgacag ttgacgtgct ctatctccaa
     2341 gcttgccgtg atgagatcgg gctgagcgag ggcgtagctg cctgaacctt cgcaacccaa
     2401 ttcattgctc accgtcaccg agtacagacc agcgttcagc cctgtgttct cgcgggcagc
     2461 agcgccattg ctccatctat atgagaagac cgtgccgggg cccatgggct gtgcagccaa
     2521 cacgccgtcg gaatcgccga aacaagtaac gggtctgtct tcggtaatac tcactgtcac
     2581 cggagcggca ggttgctgca caaaaatgct cgacacatct tcgcagccgt ccgcgttggt
     2641 cacggtcaca tcgtaaaaac cggtggcgag ttgcatggcc tcttgcgcgg catcgccgtt
     2701 gctccaggca tagccgatgg ggtttccgtt ggtactcacc gtggcgcgcc catcgttgcc
     2761 gccaaaacag gtcacgcttt gcagcaccga agccgcagcg atgaattccg ccggctctcc
     2821 gatttctatg acctgcgaag tgcggcaatt gttggcatcc gtcacgctca ggcgatagct
     2881 gcccgcgcac agtccgctga tttgtgggcc tgcggcttgc ggcccgctcc aggcgtacag
     2941 gtaggcgccc gttccgccgg tgaccgccac attcatcatt ccatcgcaaa aactgaagca
     3001 cgatgcgctg tcggcctgca acaccgcaat ggccaaagcc ggtggttccg acaccgtagc
     3061 tatgacggtg gcctgtgcaa aaacatcgct caccgtgatg cggtaatcgc ccgcccgcag
     3121 ccccggtatg gtcactgcct gcccctcggc gccgatggtt ccgttgcccg aagcactgcc
     3181 gcccacctgc tgccagatat actggtaggg cgcccagcct tcctgcaccg acagcgtaac
     3241 gacgccgtcc gcatcgccgc caccatcccg gtcggctggc ccgacggcgt gtcgttcggt
     3301 ccggtcaacc gccggccggt cgacgacttc gtccaccgca accggtggga gggcgacaag
     3361 cgcgggctcg gcc
//
LOCUS       DAORLF010000481         3368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15396_length_3368_cov_2.488379, whole genome shotgun sequence.
ACCESSION   DAORLF010000481 DAORLF010000000
VERSION     DAORLF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15396_length_3368_cov_2.488379"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>360
                     /locus_tag="PK198_24340"
     CDS             <1..>360
                     /locus_tag="PK198_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF41954.1"
                     /translation="YTDCDLVLLGPYLYHKWEGDQHLQDSGQPYRVVTIQFAIDLFSG
                     QMFQKERFIKIRNLLKEASKGIKFHGKTFEEAMPLILGLTEDRGFSNIIDFFQLLDLL
                     SESGDTTFLASEGFLPGG"
     assembly_gap    361..372
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <373..820
                     /locus_tag="PK198_24345"
     CDS             <373..820
                     /locus_tag="PK198_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF41955.1"
                     /translation="RSENSRIQVAYACILKHFTNPDFKIGDVAAQVHMSESAFSHFFQ
                     KYAFRSFTQFLVDLRIGHACKLLLDTDDTVAQISAKSGFNNLANFNRLFKKYRSCTPL
                     EYRKRYLEKTAFDWSKQPTPWQFIPSDSRTRQTVQPLEYSTRLLHF"
     gene            877..2379
                     /gene="lysS"
                     /locus_tag="PK198_24350"
     CDS             877..2379
                     /gene="lysS"
                     /locus_tag="PK198_24350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRF41956.1"
                     /translation="MQLSEQEIVRRQNLQAIRDLGIDPYPAPQFEVNAFAADIKSRYE
                     DGAEGFENLRLAGRIMMVRDMGKACFAELQDSTGRIQFYVRRDDICPGEDKRLFDELF
                     KKLLDIGDFVGIEGFVFRTRTGEITVHAKSFTLLCKSLRPLPIVKTDAEGNVYDAFVD
                     PELRHRMRYVDLVVNAGIRETFIQRTKMVNSIREFLNNRGALEVDTPVLQNIPGGATA
                     RPFITHHNALDIPMYLRIANELYLKRLVVGGFDWVYEFSRNFRNEGMDRTHNPEFTIL
                     EFYVAYKDYNWMMETTEQLLEKVALDLHGATDVQVGDKVLSFKAPFQRIGILDAIKEH
                     TGFDLYGMDEAAMRDVCRQLHIEVDNTMGVGKMVDEIFSEKCEKLYTQPTFITDYPVE
                     MSPLTKRHRSKPGLVERFELMINGKEVANAYSELNDPIDQRERFEEQVVLKDRGDEEA
                     MFIDHDFLRALEFGMPPTAGIGIGIDRLAMLMTNQSTIQEVLFFPQMRPE"
     gene            2469..>3368
                     /locus_tag="PK198_24355"
     CDS             2469..>3368
                     /locus_tag="PK198_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRF41957.1"
                     /translation="MSKLLIIGAGGVGRVAAYKCAQHPEIFSEILLASRTQSKCEVIA
                     EAVRRDTGHPNFHTARVDADNVPELVALIRDFQPAMVLHVALPYQDLTIMDACLETGV
                     HYLDTANYEPKDEAKFEYSWQWAYHDRFRDAGLLAVLGCGFDPGVTGVFTARAAHHFF
                     DEMHELDIIDANAGSHGKAFATNFNPEINIREITQKGRYFQDGQWVETEPHEIAKMIN
                     YPDIGPRKSYLIFHEELESLTKNYPTLRRARFWMTFGDEYLTHLRVIQNIGMARIDPV
                     MYKGVEIIPLEFLKAVLPDPGELG"
BASE COUNT          824 a          946 c          856 g          730 t
ORIGIN      
        1 tacaccgatt gcgacttggt gctgttgggg ccgtacctct accacaaatg ggagggcgat
       61 cagcatttgc aggacagcgg gcagccttac cgggtagtga ccatacagtt cgcgatagac
      121 cttttcagcg ggcaaatgtt tcaaaaagag cgatttataa aaataaggaa cctgctgaag
      181 gaggcctcca aaggcatcaa atttcatggc aaaacctttg aggaagccat gccgctcata
      241 ttgggcctca ccgaagaccg gggcttttcc aatatcatag acttttttca attgctcgat
      301 ctgctctccg agtcgggtga taccactttt ttggccagcg agggtttcct ccccggcggg
      361 nnnnnnnnnn nngcgctctg agaacagccg cattcaggtg gcttatgcct gcattctgaa
      421 acacttcacc aatcctgatt tcaaaatcgg cgatgtggcc gcacaagttc acatgagcga
      481 gtccgctttc agccattttt ttcaaaaata cgctttccgc agtttcacac agtttttggt
      541 ggacctccgc attggccatg cctgcaaact cctcttagat acagacgata cggtggctca
      601 gatttcggcc aaatcggggt tcaataactt agccaacttc aaccggcttt ttaaaaaata
      661 tcgctcttgc acgccattag agtatagaaa gcgatatttg gaaaaaacag cctttgactg
      721 gtccaagcag cccacgccct ggcaattcat accctccgac tcccgaaccc ggcagacagt
      781 gcagcctttg gaatactcta cgcgcttgtt gcatttttag gcaataagtt attgcagaaa
      841 ctcttacttt tgcgcctcca aaaatagaat tatccaatgc aattgtcaga acaggaaatc
      901 gtaagaagac aaaacctgca ggccatccgc gatctcggca tagacccgta tcccgcgccg
      961 caatttgaag tcaacgcttt tgccgccgac atcaagtccc gctacgaaga cggcgccgag
     1021 ggctttgaaa acctccgcct tgccggccgc atcatgatgg tgcgcgatat gggcaaggct
     1081 tgttttgccg agcttcagga ctctaccggc agaatccagt tttatgtgcg ccgcgacgac
     1141 atctgccccg gcgaagacaa acgcctgttc gacgaactgt tcaaaaaact cttggacatc
     1201 ggcgattttg ttggtataga gggctttgtg ttccgtaccc gcaccggcga aatcaccgta
     1261 cacgccaaat cgtttaccct gctttgcaaa tcgctgcgtc ctctgcccat cgtcaaaacc
     1321 gatgccgagg gcaatgtgta tgacgctttc gttgatcccg aactgcggca ccgcatgcgc
     1381 tatgtggacc tggtggtgaa tgcaggcatc cgggaaactt tcatccagcg caccaaaatg
     1441 gtgaacagca tccgcgagtt cctcaacaac cggggcgccc tcgaagtgga cacgcccgta
     1501 ctccaaaata ttcccggcgg cgctacggcc cggcccttca tcacccacca caatgcgctt
     1561 gatataccga tgtacctgcg cattgccaac gaactgtatc tcaagcgttt ggtggtcggc
     1621 ggttttgatt gggtgtatga attcagccgc aacttccgca acgaaggcat ggaccgcacc
     1681 cacaacccgg agtttaccat cctcgaattt tatgtggctt acaaagacta caactggatg
     1741 atggaaacca ccgagcaact gttggaaaaa gtagcgcttg atctgcacgg cgccaccgat
     1801 gtgcaggtgg gcgataaagt gctcagtttc aaagcgccct tccaacgcat cggcatcctc
     1861 gacgccatca aagaacacac gggcttcgat ctgtacggca tggatgaagc cgccatgcgc
     1921 gacgtgtgcc gccaactgca cattgaagtg gacaacacga tgggcgtggg taaaatggtg
     1981 gacgaaattt tcagcgaaaa atgcgaaaag ctctacacgc agcctacttt catcaccgac
     2041 tacccggtgg agatgagtcc gctcaccaag cgccaccgtt ccaagcctgg cttggtagaa
     2101 cgcttcgagc tgatgatcaa cggcaaggag gtggccaatg cttactcaga attgaacgac
     2161 ccgatagatc agcgcgagcg cttcgaggag caggtagtgc tcaaggatcg cggcgatgaa
     2221 gaggctatgt tcattgacca cgattttctg cgcgccctcg aattcggtat gccgcccacg
     2281 gcgggcatcg gcatcggtat agaccgcctc gccatgctga tgaccaacca gagtaccatt
     2341 caggaagtgc tgttttttcc gcagatgcgg ccggagtagc ggttggcggt aagcagtagg
     2401 cggtaggcgg tgcaagcgcg gaatgcggaa tattgaatcc gaaatccgaa ataatacaat
     2461 aagaaataat gtccaaatta ctcatcatcg gcgccggcgg agtcggcagg gtagccgcct
     2521 acaagtgcgc ccaacatccg gagatattct ccgaaatcct tttggccagc cgtacccaaa
     2581 gcaagtgcga ggtcattgcc gaggcggtgc gccgcgatac agggcacccc aatttccaca
     2641 ccgcccgcgt ggatgccgac aatgtgcccg aattggtggc cctcatccgc gattttcagc
     2701 cagctatggt gctgcatgtc gcgctgccct accaagacct caccatcatg gacgcctgcc
     2761 tcgaaacggg cgtacattac ttagacacag ccaattatga gcctaaagat gaagccaagt
     2821 ttgaatatag ctggcaatgg gcttaccacg accgcttccg cgacgcgggt ttgctggccg
     2881 tgctgggctg cggcttcgat ccgggcgtaa cgggcgtgtt tacggcgcgg gccgcccatc
     2941 atttcttcga tgaaatgcac gagttggaca tcatagacgc caacgcaggc agtcacggca
     3001 aggcttttgc caccaacttc aacccggaga tcaacatccg cgaaatcacg cagaaaggcc
     3061 gctattttca ggatggccaa tgggtggaaa ccgagccgca cgaaattgcc aaaatgatca
     3121 attatcccga catcgggccg cgcaaatcgt acctcatctt ccacgaggaa ctggagtctc
     3181 tcaccaagaa ctaccctacc ctgcgccgcg cccgcttctg gatgaccttc ggcgacgaat
     3241 acctcaccca cctgcgtgtg attcagaata taggcatggc ccgcattgac cccgtgatgt
     3301 ataaaggtgt ggaaatcatt ccactggagt tcctcaaagc ggtgctgccc gatcccggcg
     3361 aactgggg
//
LOCUS       DAORLF010000482         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15443_length_3361_cov_1.753176, whole genome shotgun sequence.
ACCESSION   DAORLF010000482 DAORLF010000000
VERSION     DAORLF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15443_length_3361_cov_1.753176"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK198_24360"
     CDS             <1..471
                     /locus_tag="PK198_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41958.1"
                     /translation="EDRHFAVTIWGDDPATPGIREGLLQGEVYQLRIWKRVQNSEMSI
                     APVFTSGDQAYHNDDIEILSHLNAATAVKESSIEAQSLTVFPNPTDGRFYVMLPAGIE
                     MLELRDAKGALLQRTAQPAAGPLPIETAGYAKGLLFVSAWDATGRRWLAKVVLQ"
     gene            597..2564
                     /locus_tag="PK198_24365"
     CDS             597..2564
                     /locus_tag="PK198_24365"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF41959.1"
                     /translation="MRLTLFSKYLCIAMLLPATCVVSAQTGFPWTRITTGANHTIIVP
                     ATLTATWDGMPLENGDAVGFFFERNDSLICSNYLIISGQNASMAVYGNDAPSGKNGFA
                     PGELFKAKIYRASTQQVLDAQAVFAPAGTVIPPLTITHTNMYALNGISLLTQVSATSV
                     VRPQATFDADVVCGTAPLTVQFSDQSSNAPEHWQWDFGNGQSSTEQHPTAIFTEPGIY
                     TVRLIAANAAGQDTLERQSYITAVGQVSVTVLPGDTICAGARLELQAEGAERFVWSGI
                     NLETEAGSATAATLTQAGDYVYQAIGITNDCESSPVSVAVHVRPVPVVTLSASADTIC
                     RQGALTLTATGASTYVWQGFGLSAASGSVVTVMPDTSGLLTYGVTGTANGCISALKKQ
                     EVWVRPRPQITASAERSGICLGDTVTLSAVGAEAIFWNGPGLLSFSGASVQAAPQQTG
                     TRTYQVTGNTAGCPAEGASVSIEVNSNALSASIAVSDCPGPDLMFTATVTNGGDAPNI
                     LWFRNGQPVWNGPDYTLFGAQNGMEVYCRVTPVNAPPCTTPSQALSNTVIVNCIASET
                     AELLPGIRQWSVFPNPNEGWFFVSVANTEAVSGRLRVWDMLGRPVWEERVQWSAGHQV
                     HRIELPGAAPGVYWVGLESLGQVMRKGVVVR"
     gene            2702..2872
                     /locus_tag="PK198_24370"
     CDS             2702..2872
                     /locus_tag="PK198_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41960.1"
                     /translation="MKNLRPYHLLSVVFLFVASFQIFGEDLTAKVPICSHYTQLATDT
                     TGLRNEPSLCGS"
     gene            2905..3309
                     /locus_tag="PK198_24375"
     CDS             2905..3309
                     /locus_tag="PK198_24375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF41961.1"
                     /translation="MDQILAISERELGRVSRETADILHQLARIQNFKYNSGEAIRFNC
                     EAGIGKLQRGEGIISLSRAFAYAGAKSIVTTLWAVKDKSTKDFMLAFYSNMENWPKAE
                     ALWQTRKDFLKKHKGEAAHPYYWAGFIGVGDM"
BASE COUNT          746 a          913 c          948 g          754 t
ORIGIN      
        1 gaagatcgcc attttgccgt cacgatctgg ggcgatgacc ctgcgacgcc cggcattcgg
       61 gagggtttgt tgcagggcga agtgtatcaa ttgcgcatct ggaagcgcgt gcaaaattcg
      121 gaaatgagca tcgcccccgt tttcacatcc ggcgatcagg cttaccacaa cgacgacatc
      181 gaaatattgt ctcatctgaa tgccgccact gccgtgaagg aatcctccat tgaggcgcaa
      241 tctctgacgg tatttcccaa cccgaccgac ggcagattct atgtcatgct tccggcaggt
      301 attgaaatgc tggagttgcg cgatgccaaa ggggccttgc tgcaaaggac cgcacaaccc
      361 gccgccgggc cgctgccgat agagaccgcg gggtatgcaa agggcttgct ctttgtgagc
      421 gcctgggacg caacagggcg gcgttggttg gccaaagtgg tgttgcagta gaactccgta
      481 tagagaaata tctggccaac gttggctcct cacacagcaa attcaggcgg taacttcggg
      541 ttacctcctg aatttgtgag gtacagcata tcattcaaca aaaaatacac cttcacatga
      601 ggctcaccct cttttctaaa tacctctgca tcgcaatgct gctgccggca acttgcgtgg
      661 tttctgccca aacaggcttc ccctggacgc gcatcaccac cggcgccaac cacaccatca
      721 ttgtgcctgc caccctgacg gctacctggg atggaatgcc tttggaaaac ggagatgctg
      781 ttggtttctt ttttgaacgc aacgacagtc tgatttgcag caattacctg atcatcagcg
      841 gccaaaacgc ctctatggcc gtatatggca acgatgcgcc atccggcaaa aacggctttg
      901 ctccgggaga gttgttcaaa gcaaaaatat accgggcctc tacccagcaa gtgctggatg
      961 cgcaggcggt gtttgctccg gcgggtaccg ttatacctcc gctgaccatc acccatacca
     1021 atatgtatgc gctcaacggc atcagcctgc tgacgcaggt ttcggccacg tcggtcgtgc
     1081 gtcctcaagc tacatttgat gccgacgtag tgtgcggcac ggcgcccctg acggttcagt
     1141 tttcggacca aagcagcaat gcccccgaac attggcaatg ggatttcggc aacgggcaga
     1201 gttcgacaga gcagcatccc acggcgatat ttaccgagcc gggtatttac accgttcgcc
     1261 tcatagctgc caatgccgcc gggcaagaca cgctggagcg ccagagttac atcaccgcag
     1321 tgggtcaggt gtcggtgacg gtgctgcctg gggataccat ttgcgcagga gccaggctgg
     1381 agcttcaggc cgaaggggcg gagcgttttg tgtggtcggg cataaatttg gaaacggaag
     1441 caggctcggc aacggcggcc acgcttacac aggcaggcga ttatgtgtat caggccattg
     1501 gaatcaccaa tgattgcgaa tcatcgccgg tttcggtagc ggtacatgtg cgcccggtac
     1561 ctgttgtaac gctcagcgct tccgcagata ccatttgccg gcaaggcgcc ctcacgctca
     1621 ctgcaacggg ggcaagcacc tacgtctggc agggctttgg gctgtctgct gcaagcggca
     1681 gtgtggtcac ggtaatgccc gatacctccg gccttcttac ttacggcgta acaggtactg
     1741 ccaacggctg catttcggca ttaaaaaagc aagaagtatg ggtgcggccc cggcctcaaa
     1801 tcacagcctc tgccgagcgg tcaggtatct gtctgggcga tacggtgacc ttgagcgcag
     1861 tgggtgccga agctattttt tggaacggcc ccgggctgct gtctttcagc ggagcatcgg
     1921 tacaggcggc gcctcaacaa accggcacaa ggacttatca ggtaacgggc aacactgccg
     1981 gatgccctgc cgaaggcgcg tccgtttcca ttgaggtgaa cagcaacgcc ctctccgcat
     2041 ccattgccgt gagcgactgc cccggccccg atctcatgtt caccgccacc gtaacgaatg
     2101 gcggcgatgc gcccaacata ctgtggtttc gcaatgggca gcccgtgtgg aacggcccgg
     2161 attacacctt atttggcgca caaaacggca tggaagtgta ttgcagagtg actccggtga
     2221 atgccccgcc ctgtactaca ccgtcgcagg cgctgtcgaa tacagtaatc gtcaattgta
     2281 tcgcatcgga gacggcagag ttgctgcccg gtatccggca atggagcgtg tttcccaatc
     2341 ccaatgaggg ttggtttttc gtatccgtag ccaacaccga ggccgtcagc ggcaggctgc
     2401 gggtatggga tatgttgggc cggccggtat gggaagagcg ggtacaatgg tcggcaggcc
     2461 atcaggttca tcgcatagag ctgcccggcg cggcgcccgg cgtttattgg gttgggttgg
     2521 aaagcctggg gcaggtgatg cggaaggggg tagtggtgag gtgaggattt tatacaagat
     2581 acttggatcc tccggttctc ttcaggccgg gggattttta ttttgctcat cgtatgccgg
     2641 ttaacgaaag agcgcttatt tttacgctgt atatacactt tcctaaagcc tgttattcat
     2701 tatgaaaaac ctgcgccctt atcatctcct ttctgttgtc ttccttttcg ttgcatcgtt
     2761 tcagatcttc ggcgaagacc tgacggcgaa agtccctatc tgttcacatt acactcagct
     2821 tgcaaccgac accaccggcc tccgcaacga gccctcgctc tgcggctcgt agatgagcgc
     2881 atgttcaccg aggcacaatt gttattggat caaatcctgg ccatcagtga acgggagctg
     2941 gggcgagtga gccgggaaac cgccgatatt ctgcaccaat tggcaagaat tcagaatttt
     3001 aaatacaatt ccggggaagc gatccgattc aactgtgagg ccggtatcgg caagctacag
     3061 cgcggcgagg gcatcatttc tttgtccaga gcatttgcgt atgccggggc caagagtatt
     3121 gtcaccacgc tttgggccgt aaaggataag agtactaaag acttcatgct cgccttttac
     3181 agcaatatgg aaaactggcc caaggcggag gccttatggc aaacgcgcaa agactttttg
     3241 aagaaacaca aaggcgaggc cgcgcacccc tactactggg ccgggtttat cggcgtgggg
     3301 gatatgtgat aaaaaaactc atctctctct ctttagtagt ggggcaaaaa taaatgctca
     3361 t
//
LOCUS       DAORLF010000483         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15455_length_3359_cov_1.862893, whole genome shotgun sequence.
ACCESSION   DAORLF010000483 DAORLF010000000
VERSION     DAORLF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_3359_cov_1.862893"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>2676)
                     /locus_tag="PK198_24380"
     CDS             complement(176..>2676)
                     /locus_tag="PK198_24380"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF41962.1"
                     /translation="HTLLEETVHRYLQQHRDADISISPKAVWLLDPVRIITGMSVVGM
                     WQKRFESIIRHIIGPAEWTDKPDVMVVDNPVALLRIGRYSGGNLALADVLKPYLEARR
                     FPFLLLATPEEWSILQEQDRRFSSLFQVIRIDEAPRDDALRMLLEKRKSLEREYGSAF
                     RIEAVDQLINIHRNYLSSRVLPGSVAKMMERLSVKFRHQTIDAPDVLREFQNASGLSE
                     VFLDDTQMLSEEEVRAGLESMLVGQPEALDVLAGVVMLIKSKLADKTKPLASMLLAGP
                     TGVGKTQAAKALCRMLTGSESALMRFDMNEYIDGSAVQRLIGSFAQPEGQLTSTVRHN
                     PFGVLLLDEIEKANPKVHDLLLQLLDDGRLTDSLGRTVDFSNVVVVMTSNLGAADATS
                     VISFGHSEEDISTVYVRAVEKFFRPEFVNRIDNIIPFRSLDEEQIYQIARLQLREILQ
                     RDGFVRRNSILNVDQNALRWVSARGYDKKMGGRALKRQIESDLTELSADQLITIPPGD
                     SIILDISLVNERLQPRIRSIRYALSMSGLLPEVPDEAKGKSFLQQLQRRVDAFRKRLA
                     DSAKRAAVHSNKITTNDVDYFELKENVESLLEEIQHLHLSYKGEYLTLHKPVSPLRTK
                     QPAFANSSRNFHQEKTSRDEMVQEVRESYRFGLPHFNSAKTIFTDLLIRLEFLQIQLY
                     AFLEGRGKDKIELRVSSMANGAGRDEAQYLLECYEALFRHWDVAFRVFKNAGKIEATG
                     FGLTTILKPEAGIHLFNSPHDSGIPILLSIQDAGQSNDARTDMERLEIIREYDQFKLL
                     SDVRSGLINDMQTTVPEMQLLLYCGMKAVGGVQG"
     assembly_gap    2677..2696
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2697..>2975)
                     /locus_tag="PK198_24385"
     CDS             complement(<2697..>2975)
                     /locus_tag="PK198_24385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41963.1"
                     /translation="VFQSPRRQYITSIDVSVQLKTGPFSFERADYADFFMRMNSSFDF
                     EGGEELLKTSTELNNRFPSELKRAHYRDDWADMLYHRLFSPDHVPLALV"
     assembly_gap    2976..3088
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3089..>3359)
                     /locus_tag="PK198_24390"
     CDS             complement(<3089..>3359)
                     /locus_tag="PK198_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41964.1"
                     /translation="LQQPVATHARYERAVELYKKDLDNVFKEYRLSRANEDMLFWLMF
                     QPKVHFAKKTLHIRTGKSIVSGMFTIAHFEVGGYTFVLLPDADNY"
BASE COUNT          701 a          864 c          880 g          781 t
ORIGIN      
        1 gttcaagatc gttttgtgag tagtcgcgag cttcgggcct tgagccggat gctccgcgat
       61 tgaaaaaagc aaagtccgag tacaaacacc gtcgagatgg gatggttcaa agggtttcgg
      121 gttcaaggtt tcgggttcca gatttcgggt tgctcacccc tcacacactc acccctcacc
      181 cctgaacccc tcccaccgcc ttcatcccgc aatacaacaa caactgcatc tccggcaccg
      241 tcgtctgcat atcgttgata aggcccgagc gcacatccga caggagtttg aactggtcat
      301 actctcgtat gatttccagc cgctccatgt ctgtacgggc gtcgtttgat tgcccggcat
      361 cctgaatgga aagcaaaatg ggaataccgc tatcgtgcgg cgaattgaag aggtgaatgc
      421 ccgcttccgg tttcagtatg gtcgtcagcc caaaccccgt agcctctatc tttccggcgt
      481 ttttgaatac ccgaaaagca acatcccaat ggcggaagag cgcctcgtag cattccagga
      541 gatactgcgc ctcatcgcga ccggcgccgt tggccattga tgagacgcgg agttcgatct
      601 tgtctttacc ccggccttcg aggaacgcat ataattgaat ttgaagaaat tccaaccgaa
      661 tcagcaagtc ggtaaaaatg gtttttgcac tgttgaaatg aggcagccca aagcgatacg
      721 actcgcgtac ttcctgcacc atctcgtcgc gggaggtctt ttcttggtgg aaattccggg
      781 agctgttggc gaaagccggc tgcttggtgc gcagcggcga taccggcttg tgcaaggtca
      841 gatattcgcc tttgtaagac aagtgcaggt gctgaatttc ctccagcaac gactcgacgt
      901 tttctttgag ttcaaaataa tccacatcgt tggtggttat tttgttgctg tggacggcgg
      961 ctcgtttggc gctgtctgcc aggcgtttgc ggaaggcgtc cactcggcgt tgcaattgct
     1021 gcaaaaaaga tttccccttg gcctcatccg gtacttctgg caacagcccg gacatggaca
     1081 gggcatacct gatgctgcgg atgcgcggtt gcagacgctc gtttaccaac gagatatcta
     1141 agatgatgga atcgccgggc gggatggtaa tcaattgatc ggcagagagt tcggtgaggt
     1201 cgctttcgat ttgccgcttg agcgccctgc cgcccatttt tttgtcgtac cctctggctg
     1261 atacccagcg cagggcgttt tggtcaacat tgaggatgga gttgcggcgc acaaaaccgt
     1321 ctcgttgcaa tatctcgcgc agttgcagcc gcgcgatctg atagatttgc tcttcgtcta
     1381 aggatcggaa cggaatgatg ttgtctatgc gattgacaaa ttccggcctg aaaaacttct
     1441 ccaccgcccg cacatacacc gtgctgatgt cttcctcgct gtgcccgaaa ctgatgacgg
     1501 aggtagcatc cgccgcgccg aggttggagg tcataaccac caccacattg gaaaaatcaa
     1561 cggtgcggcc taagctgtca gtgaggcgcc cgtcgtctag cagttgcaac aggaggtcgt
     1621 gtactttggg gttggctttc tcaatttcgt ctaacagcaa taccccgaaa ggattgtgcc
     1681 gcacggtcga ggtgagctgg ccttcgggct gtgcaaaact gccgatgagg cgctgcacgg
     1741 cgctgccgtc tatgtactcg ttcatatcga agcgcatcag ggccgattcg ctgccggtga
     1801 gcatcctgca caaagccttg gctgcctgtg ttttacctac gccggtaggg ccggccagca
     1861 acatagacgc taggggcttg gttttgtctg ccaattttga tttgatcagc atcactacgc
     1921 cggcgagtac atccaacgct tcgggttggc ccaccaacat gctctccaaa cccgcgcgca
     1981 cctcttcttc cgagagcatc tgcgtgtcgt ccaagaagac ttccgacagg ccgctggcgt
     2041 tttgaaattc gcggagcaca tcgggcgcat ctatggtctg atgcctgaat ttgaccgaca
     2101 agcgttccat cattttggcc acacttcccg gcaaaaccct gctgctgagg taattgcgat
     2161 gtatgttgat caattgatct accgcctcta tgcgaaatgc gctgccgtac tcgcgctcca
     2221 gcgattttct tttttccaac aacattcgga gggcgtcgtc gcggggcgct tcgtctatac
     2281 gaatgacctg aaacaggctg ctgaatcgcc ggtcttgctc ctgcaaaatg ctccattctt
     2341 cgggtgtagc cagcagcaga aagggaaacc ggcgcgcttc gaggtagggt ttgagcacgt
     2401 cggccaaggc cagattgccc cccgaatagc ggccaatgcg caacagagcc acggggttgt
     2461 ccaccaccat cacatcgggt ttgtcggtcc actcggccgg gccgatgatg tgccggatga
     2521 tggactcaaa acgcttttgc cacatgccca ccacgctcat gccggtgatg atgcgcacgg
     2581 gatcgagcag ccacaccgct ttggggctga tggaaatatc ggcgtcgcgg tgttgttgca
     2641 aatagcgatg taccgtctct tcaagcaaag tatggcnnnn nnnnnnnnnn nnnnnnacca
     2701 aggccagcgg gacatgatcg ggtgaaaaca ggcgatgata gagcatgtcg gcccagtcgt
     2761 cgcggtagtg agcgcgtttg agttcggagg gaaagcgatt gttcagttca gtgcttgttt
     2821 tcaacagttc ttcgccgccc tcgaaatcaa aactgctgtt catgcgcata aaaaagtcgg
     2881 cataatcagc acgttcgaat gaaaatggtc ccgttttcaa ttgcacactc acgtctatgg
     2941 aggtgatgta ctgccgccgg ggcgactgga agaccnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ataattgtct gcatcgggca ggagtacaaa
     3121 tgtgtagccg cccacttcga agtgcgcaat ggtaaacatg ccggagacaa tgctttttcc
     3181 ggtgcggata tgaagcgtct tttttgcaaa atggactttg ggctgaaaca tgagccagaa
     3241 gagcatgtct tcgttggccc gactgagccg gtattctttg aatacgttgt ctaaatcctt
     3301 tttgtacaat tccaccgccc gctcgtagcg cgcatgggtg gccaccggct gttgtaaaa
//
LOCUS       DAORLF010000484         3347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15526_length_3347_cov_1.858445, whole genome shotgun sequence.
ACCESSION   DAORLF010000484 DAORLF010000000
VERSION     DAORLF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15526_length_3347_cov_1.858445"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..798
                     /locus_tag="PK198_24395"
     CDS             166..798
                     /locus_tag="PK198_24395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF41965.1"
                     /translation="MTKRSFHPSDIQAIKEEYLDGIFQFVARFLQLHPDEKQAIAEQT
                     NLRYFPKGSTLLREGQVSNLCYFVLRGCVRQYYLVDGVEKTTHFFTEGQPVTPDESTN
                     LLRPSQSYLSCLEDSVLAVSTPEKEQQMYRKFPRLESVCRLSVEEELGKSKAHLASFI
                     LLTPEQRYLQLLQNQPDLIARVPNIHLASYLGIAPESLSRIKKRLQERIS"
     gene            866..1825
                     /locus_tag="PK198_24400"
     CDS             866..1825
                     /locus_tag="PK198_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRF41966.1"
                     /translation="MKAAVCTQYGPPEVLHIQTLERPVPKPGEILIKIHAAAVNSGDC
                     RLRRADPAAVRLFLGLFRPRKKVLGDAFSGEVMEVGAQVTRFRPGDQIFGTTGLRLGA
                     FAEYVCLPAEGISLVLKPADTPHEWTAATPFGYNTALHFWEKAGLKAGQKVLVYGASG
                     SVGSAAVQLAKHLGAEVTAVCSAANIELVQSLGADRIIDYATTDFAAQGAVYDIVFET
                     VNKTSVKTCLSAVKPGGTLILGSAMLTETLQGAWAGLTSGKKVISGVALDTQSRLDKL
                     QTLLQNGAIQPVIDKVFPLEEIVEAHRLTDSGRKRGNVVLKMR"
     gene            complement(1843..2478)
                     /locus_tag="PK198_24405"
     CDS             complement(1843..2478)
                     /locus_tag="PK198_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41967.1"
                     /translation="MKKHLLFLLALIAFSSAAWADSPITSTPIHEAYQDIGIVSKASA
                     ENAKFDADMLAFLSDAAKPIDQKVALCSALGWQMKGKAKLFSDHLSKRSGWDLKKMSG
                     DEMLVLSYLMALDDYNNMDLPLALASKALAKNKQSYTFRMIHALILAQKQFDSNWCKV
                     FTTVAAVERNKSLKQDLRDGAVNIIFDYINLYQDSCKGGKGVKNPPVLMRG"
     gene            complement(2556..3278)
                     /locus_tag="PK198_24410"
     CDS             complement(2556..3278)
                     /locus_tag="PK198_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRF41968.1"
                     /translation="MNRIPQNQPGFQRTAPAPTPVNTLSDAERKAGWKLLFDGQSLKG
                     WRNYGKKTIGSSWKIDDGAIHLAAKKNPNGGWQAPDGGDIITDGEYENYELRLEWKIG
                     ACGNSGIIYNVEESDKYQYPWQTGPEMQVLDNACHPDSKIRTHRAGDLYDLIECKYET
                     VKPAGEWNRVRLVVNKGKVQHWLNGRKVVETEMWTDEWRKMVAASKFKDMPGFGTVRK
                     GHLCLQDHGDPVWYRNVKIKEL"
BASE COUNT          748 a          887 c          880 g          832 t
ORIGIN      
        1 gagcgcggct ggcagacgta cgcgccgcca aacttggaat ggcgcaaggc catgcaggcg
       61 caagctgtga cggctgaatg aaataccatc cgttttatct aaaaaaacga gctatttaga
      121 tgtggtaaaa cctacctttg ccgccgattc aatccattgc acaccatgac caaaaggagc
      181 ttccatccat ctgatattca ggcaataaaa gaagaatacc tcgacggcat ttttcagttt
      241 gtcgctcgtt ttcttcaact ccatcctgac gaaaaacagg ccatcgccga acaaaccaat
      301 ctccgttatt ttcccaaagg aagcacgctg ctgcgcgaag gacaggtttc caacctttgt
      361 tatttcgttt tgagaggctg cgtgcggcag tactatttgg tggacggcgt agaaaaaacc
      421 acccattttt tcacggaggg tcaacctgtt acgccggatg agagcaccaa tctgctgcgg
      481 ccttcgcagt cctatttatc atgcctggaa gacagtgtat tggccgtcag cacgcccgaa
      541 aaagagcagc agatgtatcg taagtttccg cgtttggagt cggtctgccg attgtcggta
      601 gaagaggagt taggcaaaag caaagcacat ctggcttctt tcatcctgct gactccagag
      661 cagcgatacc tgcaattgct tcaaaatcaa cctgatctga tcgcccgtgt gccgaatata
      721 caccttgcct cctacctcgg catcgcaccg gaatcgctga gccgcatcaa aaaaagatta
      781 caggagcgca tttcttaact caagtcaatg tgcggcagtt gggtttccct gcaactttgc
      841 agcgtcaatc aaaccaatct cgaccatgaa agctgccgtt tgcacccaat acggaccacc
      901 tgaagtcctt cacattcaga cacttgagcg ccccgtaccc aagccgggcg aaatcctcat
      961 aaaaatacat gctgctgccg tcaactccgg cgactgccga ctgcgcaggg ccgatcccgc
     1021 tgccgtgcga ttgtttctgg gcttgttccg gccccgaaaa aaagtactcg gagatgcctt
     1081 ctccggagag gtgatggaag tcggcgcgca ggtaacgcgc tttcgtcccg gcgaccagat
     1141 tttcggcacc accggcctgc gtttgggcgc tttcgcggaa tacgtctgcc tgcctgcaga
     1201 gggcatctcg ctggtcttga aacctgccga taccccgcat gaatggactg ctgcaacgcc
     1261 attcgggtac aataccgctt tgcatttttg ggaaaaagca ggtctgaagg ccgggcaaaa
     1321 agtgttggtt tacggcgctt ccggttcagt aggttcggca gcggtacaat tggccaaaca
     1381 cctcggcgca gaggtaacgg cggtgtgcag cgctgccaat atcgaattgg tacaatcgct
     1441 tggcgctgac aggattatag actatgcaac aaccgacttt gccgctcagg gcgctgtgta
     1501 cgacattgtg tttgagaccg tcaacaaaac aagcgtaaaa acctgtttat cggcagtcaa
     1561 acccggcggc acgctcattc tcggctcggc catgctcacc gagactttgc aaggcgcctg
     1621 ggccggcctc acttcgggca aaaaggtgat ttccggcgtg gctttggata cacaatcgcg
     1681 attggacaaa ctgcaaacat tgctccaaaa cggcgccata cagcccgtca ttgacaaggt
     1741 ttttcctttg gaagaaatcg tcgaagcgca ccgtttaaca gactccggca ggaaacgggg
     1801 caatgtggta ctgaaaatgc ggtgaatctg gttggagacg gattaacctc tcatcagcac
     1861 cggcggattc ttcacacctt tgccaccctt gcagctatcc tgatacaaat tgatgtaatc
     1921 aaaaatgatg ttgacggctc cgtcgcgcag gtcttgtttc aagctcttgt tacgctctac
     1981 cgccgccaca gtggtaaata ctttgcacca attggaatcg aactgctttt gagccagaat
     2041 gagcgcgtgg atcatcctga atgtgtagct ctgcttgttc tttgccaatg ccttggatgc
     2101 caatgccagc gggaggtcca tgttgttgta atcatcgaga gccatgaggt agctgagtac
     2161 cagcatttca tcgccgctca tctttttgag atcccagccg gagcgcttgc tcaggtgatc
     2221 tgaaaaaagt ttggccttgc ccttcatttg ccagcctaag gcactgcaca gcgccacttt
     2281 ttgatctatc ggcttggctg cgtcggacag aaaggccaac atgtctgcat cgaatttggc
     2341 attttctgcc gaggctttgg atacgatgcc gatgtcctga tatgcctcgt gaatgggcgt
     2401 ggaagtgatg ggcgaatcgg cccaggctgc gctcgaaaat gcgatcagcg cgagtagaaa
     2461 aagcaggtgt tttttcatgg tttttaatgg ttttatggtg attgtggagc ttgcatgcct
     2521 gaaacgatag acacgcaagc tccttagtca ttgatttaca actccttgat ttttacattt
     2581 ctgtaccaaa ccgggtctcc gtgatcctga agacagaggt ggcccttgcg caccgtgccg
     2641 aagccgggca tgtctttgaa tttggatgcc gcgaccatct tccgccattc gtcggtccac
     2701 atttcggttt ccaccacttt gcggccgttg agccagtgct gcactttgcc tttattgacc
     2761 accaatcgca cgcggttcca ctcgccggca ggttttacgg tttcgtattt gcactcgatg
     2821 aggtcataga ggtcgccggc gcggtgggtt cggattttgg aatccgggtg gcaggcgttg
     2881 tcgagtacct gcatttcggg gccggtttgc cagggatatt ggtacttgtc ggactcctca
     2941 acgttgtaaa tgatgccgct gttgccacag gcgccgattt tccattccag acgaagttcg
     3001 tagttttcgt actctccatc ggtgatgata tcgccgccgt ccggagcctg ccagcctccg
     3061 ttggggttct tttttgcagc caaatgaatg gcgccgtcgt caattttcca actggagcca
     3121 atggtctttt taccgtagtt gcgccagcct ttgagcgatt ggccgtcgaa taggagcttc
     3181 cagccggcct tgcgttctgc atcggagagc gtattgacgg gcgtcggagc gggcgcagtg
     3241 cgttggaaac caggctgatt ctgtggaatg cggttcatgg tgtaccaagc ctcggtgctc
     3301 cacagttcga ggtcgccgtc gccggtccac caataaggca ggcgaat
//
LOCUS       DAORLF010000485         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15578_length_3338_cov_2.177581, whole genome shotgun sequence.
ACCESSION   DAORLF010000485 DAORLF010000000
VERSION     DAORLF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15578_length_3338_cov_2.177581"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1343)
                     /locus_tag="PK198_24415"
                     /pseudo
     CDS             complement(194..1343)
                     /locus_tag="PK198_24415"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
     gene            complement(1479..2429)
                     /locus_tag="PK198_24420"
     CDS             complement(1479..2429)
                     /locus_tag="PK198_24420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF41969.1"
                     /translation="MNSPLISVVVTVYNEEDNVRPLLEQLHAALAALDYELVYVDDGS
                     RDNTIAELRAAAHPRLRVVELRKNYGQSLALMAGIDVARGEYIATMDGDLQNDPSDIP
                     AMLQMAQTGEWDMVAGNRAKRQDKVLLRKIPSAIANYLIRQLSGVHLKDYGCALKVFR
                     AELAKELGLYGELHRFIPVLASLEGARITQVDVKHHPRTAGRSKYGLGRTFKVVSDLL
                     LMIFFKRYLQKPMHLFGNAGVFLFALGAVINLYLGVLKLMGNDIWGKPLLILGLMLVL
                     AGIQFVTIGIVVEIQMRTYFEAQQKKPYKIRHIHQTPEKE"
     gene            complement(2691..>3113)
                     /locus_tag="PK198_24425"
     CDS             complement(2691..>3113)
                     /locus_tag="PK198_24425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41970.1"
                     /translation="RSRIEQFLTLFNQAWVMEQKYILDLQEVPEGFEDIAEYLQRPLQ
                     EEEIRAKLRAEEEVEAIFAQQEADKAYYKRTAEEERRQKEEAKAREKEAKAREEQALE
                     QAREARIKLARLLLRTGMSVKDAVLETGLSEEELRGLE"
     assembly_gap    3114..3222
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          682 a          974 c          886 g          687 t
ORIGIN      
        1 ggtttcgggt ttcatccgcc gtagccttag cgaaagcggg ttcaaggttc cgcgtttcgg
       61 gttcaaggtt tcgcccgacg ataaaccgcg cagcagcttc ctaacccgtt tctaccccga
      121 cgataacctg cgaagcagat taccagcccg ttcctacccc gacgataacc tgcgaagcag
      181 attcctaaac cgcctaccgc ctaccgactc tcctccccag cagcaacgtc aaagccaccg
      241 tgatccccgt ttgcacccac cagggcattt gcgccccgat attgggcagc acgccggata
      301 ccgatttcca cagattttga agccccagaa aacaagtcac tgcaaacacc agcaacagca
      361 acacattggc cgtcagtccg ttggcgtact ccgccggcag tagttttcgg tcgtttactg
      421 ccagcagcac aaacgccgtc accaaaggca gcaacaggcc gttgatggct tgcgccaaaa
      481 tgatggccgg aatgggcttc acattcagca atccaaaaac aagcccgatg cccaaaacga
      541 gtacccacac ccaccggaaa tgcgccggtt tccagcccgc gcgattgccc agcaggcttt
      601 gcgccgtcat ggctgccgcc aatggcgccg tgatggccga ggtagcgccc gccgcaaaca
      661 atccgaatcc gaacagcacc gaacccgaag gtcccagccg atttcgcaag gtatcggcca
      721 ctgcggcata gctgaactcg cccaccaccc cggcgcccac cgccacaatc gccatagaaa
      781 taagcccccc gatgagtatg gccggcgcca gtcctgcccg catctcggcc accgattgcc
      841 ccttgccgat gcccgctgcc aaaaataagt tgtacggcac aattgtagtg cctatcaggc
      901 cggtaatgat gagcaaagaa cccgccggaa cacttggtac cacagcgccc ttgagccaat
      961 cgcccgccga acccggaccg gtgatggccg agtaaataaa ggcaaatcag aaacaccgct
     1021 acgcccagta tgcgggcaat gagcgcatgc cggccgctcc acaacaacag cgccgccgga
     1081 aaagccacac acaaagccgc taccggtctg gcgacaggaa ccaaagaccg cagtcccgca
     1141 gcagcgccca ctatgttgcc ggcctcgtaa gccgcgcaac caaacgccac cgctgcaaac
     1201 agcgccacgc acagcgcgcg gccgcgctcc gcataccgca gggcaatgat ttcgcctaag
     1261 ctcttgcccg atgcaatggt gatgcgcgcc gccgcttcct gcaacacaat ggccgcagcg
     1321 atggaaaacg tgagcgccca catcaactga aaaccgaagg atgcgcccgc ctgcgctgcc
     1381 gtcgtcacgg tacccggccc gatgaacgcc gccgatatga ccgaccacag caacacgctg
     1441 ccgaaaccct gccggaagga gatcattttt ggcatagctt attctttttc aggggtttgg
     1501 tggatgtggc gaatcttgta gggcttcttt tgttgcgcct caaaataggt gcgcatttgt
     1561 atttccacta caatgccgat ggtgacaaat tggatgccgg ccagcaccag catgaggccc
     1621 agaatgagca ggggtttgcc ccaaatgtcg ttgcccatta gtttgagtac gcccagatac
     1681 aggttgatga cggcgcccag agcgaacaaa aacacaccgg cattgccaaa gaggtgcatg
     1741 ggtttttgca ggtagcgttt gaaaaaaatc atcagcaaca ggtcgctcac caccttgaaa
     1801 gtgcggccca gaccgtactt cgagcggccc gccgtgcgcg ggtggtgctt gacgtccacc
     1861 tgcgtgatgc gggcgccttc gaggctggcc agcacgggaa tgaagcggtg gagttcgccg
     1921 tacaggccca gttctttggc caattcggcg cggaacacct tgagcgcgca gccgtagtct
     1981 ttgaggtgta cgccggagag ttggcggatg aggtagttgg cgatggcgct ggggattttg
     2041 cgcagcagca ccttgtcttg gcgcttggcg cgattgccgg ccaccatgtc ccattcccca
     2101 gtttgggcca tttgcaacat ggcgggaatg tcggaggggt cgttttggag gtcgccgtcc
     2161 atggtggcaa tgtactcgcc gcgcgccacg tctatgcccg ccatgagggc cagactttgg
     2221 ccgtagtttt tgcgcagttc caccacccgc aggcgcggat gagcggcggc gcgcagttcg
     2281 gcgatggtgt tgtcgcggga gccgtcgtcc acatacacca gttcgtaatc gagggcggcg
     2341 agggcggcgt gcagttgttc cagcaggggc cgaacgttgt cttcctcatt ataaacagtg
     2401 acgacgacgg aaataagcgg cgaattcatg tatttgattt tcaatgtttt cgtaatgatg
     2461 cgagtacagc gtgtgtgatc tgtttttccc ctgcgaagcg tgtgaacttg gcgagctggt
     2521 cagggatagg tcaggttgtc caccttgatg acttccaaac aaacggcggc aataccctgc
     2581 ggcgagggtg gttttgggca gtttgagttc cagagacagt tgcagacttc ttccccgatg
     2641 ttgcagccga aggtgccggg gtcgttgatg tggagcaggc gggagatggc ttactccaac
     2701 cccctcagtt cctcctccga cagccctgtt tcaagcacag catctttgac ggacataccc
     2761 gtgcgcagca gcaggcgggc taatttgatt cgggcttcac gcgcttgctc aagtgcttgt
     2821 tcttctcgcg cttttgcttc tttttctcgc gcttttgcct cttccttctg ccgacgttct
     2881 tcttctgccg ttcgcttata gtacgcctta tctgcctcct gctgagcgaa aatggcttcc
     2941 acttcttctt ctgcgcgcaa cttggcgcgt atttcttcct cctgaagtgg ccgctgaagg
     3001 tactccgcta tgtcttcaaa gccttccggc acctcttgca aatcgaggat gtatttttgt
     3061 tccatgaccc aggcttgatt gaataatgtg agaaattgct cgatgcggct gcgnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgtccat gatcttgtgg
     3241 ttcaccttga cggcaacgtg cggaatgtct tccacattgt atcccagtat gtagatggag
     3301 atgatgggca gggcgctgtc gtgttctacc tgtgtttc
//
LOCUS       DAORLF010000486         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15582_length_3337_cov_1.988117, whole genome shotgun sequence.
ACCESSION   DAORLF010000486 DAORLF010000000
VERSION     DAORLF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15582_length_3337_cov_1.988117"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK198_24430"
     CDS             complement(<1..778)
                     /locus_tag="PK198_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41971.1"
                     /translation="MNILLIGSGGREHALAWKMKQSQLCSRLLIAPGNSGTAACGENV
                     AIRPDDFQGLKALALREQIDMLVVGPEDPLVLGIWDFFKNDPDLQHIIVVGPSQEGAR
                     LEGSKAFAKAFMQEFGIPTAAYREFDAAQMEEGLAYIAAHSLPVVLKADGLAAGKGVL
                     ICTDTDEAQREFERIVARYVDLADVGERLYAVESRNIGFFGYGVKGMTLSMIEAAVEI
                     TDARISGADVHRIVALAKDLLRHPVEVLDGDMRQRRHRDRV"
     gene            complement(1081..1530)
                     /locus_tag="PK198_24435"
     CDS             complement(1081..1530)
                     /locus_tag="PK198_24435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41972.1"
                     /translation="MTLRFALLALAITLSPVVLSAAVQYSAPVSQTVGTAPRLPRKKS
                     FFPNEHARRYHYLQLRRDTGAAARFVLLAILIGLVLYGLLYYLIYAGEGGIYGWTWGV
                     LLSAIGGVVIGVIVVMIIRWLMFWLSGLVFRLRVPAFRRRYRRCPVR"
     assembly_gap    1743..1873
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1874..>2091)
                     /locus_tag="PK198_24440"
     CDS             complement(<1874..>2091)
                     /locus_tag="PK198_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41973.1"
                     /translation="LDIGFRRDGSSVSISDQGDLSIFHAFDEVDARGMRLTPGKTYSK
                     VIPDMAAAQNLDLLQLLRQGYTELQMTK"
     assembly_gap    2092..2247
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2248..>3082)
                     /locus_tag="PK198_24445"
     CDS             complement(<2248..>3082)
                     /locus_tag="PK198_24445"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF41974.1"
                     /translation="RHAAHQSAAPTDGADFSMYKALYDRHSAYKEPSITRQRFKHAAI
                     APLVQRLQSPFQWSVDGKSIEGRNIYRVQYGNGPIKVLLWSQMHGDEPTATMALMDIF
                     NFLSRSDEWDELRSRLRSELTLVFVPMLNPDGAERFERRNALGIDLNRDALRLQCPES
                     QLLKRLRDELSAQWGFNLHDQGRFYGAGLGPKNAAMAFLAPAYNYEKEINDSRGNAMQ
                     LIAGLSAFLHEYIPGHIARYDDEFEPRAFGDNIQKWGTSTILIEAGVLPNDPEKQELR
                     RL"
     assembly_gap    3083..3117
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          592 a          777 c          991 g          655 t
ORIGIN      
        1 agacccggtc gcggtggcgg cgctggcgca tgtcgccgtc cagcacctcc accggatgcc
       61 gcagcaggtc cttggccaag gcgacgatgc gatgcacgtc ggcgccgctg atgcgcgcgt
      121 cggtgatttc caccgcggcc tcgatcatcg acagggtcat acccttcacg ccgtagccga
      181 agaagccgat gttgcggctc tccaccgcgt acaggcgttc gccgacgtcg gccaggtcga
      241 cgtagcgggc gacgatgcgc tcgaactcgc gctgcgcctc gtcggtgtcc gtacaaatca
      301 gcacgccctt gccggctgcc agcccgtcgg ccttcagcac taccggcaga ctgtgcgcgg
      361 ctatataggc caggccttct tccatctgcg ccgcgtcaaa ttcccggtac gccgcagtgg
      421 gtatgccgaa ctcctgcata aaggctttgg caaatgcctt gctgccctcc agccgggcgc
      481 cctcctgcga ggggccaact acgatgatgt gttgcaaatc ggggtcgttt ttaaaaaaat
      541 cccaaatgcc gagcaccaga gggtcttcgg ggcctaccac cagcatatct atttgctcgc
      601 ggagggccag cgctttaagt ccctggaaat catcgggtcg tatggctacg ttttcgccgc
      661 aggcagcggt accagagttg ccgggcgcga tcagcagacg gctgcaaagt tgactttgtt
      721 tcattttcca ggccaaggcg tgctctctgc cgccgctacc gatgaggagt atgttcatgt
      781 tgttttttgc tgcaaagata gtgtttcggg tttaaggttt cgggttcgat gttttgcatt
      841 tcgggggtag tgaagggggg acccggtaag tttcagcggg atagtcaccc tttcactaag
      901 ctggacagtt gtttataatt gttatgcccc ctcgcgttgc ggcaggggca gtagcggtac
      961 tggcggcgtc tttaaacgcc ggcagtaaga gcggatgacc cgacacgccg ttgagatggc
     1021 ctgcctgccg ggcattgagc aggggcaggc tacaggtggc gtgggccgcc ccaaaaaaaa
     1081 ctacctcacc gggcaacgcc ggtacctgcg ccgaaatgcc ggcacgcgca gccgaaatac
     1141 caagccgctg agccaaaaca tcagccaacg aatgatcatg acgacgatga cgccaatgac
     1201 taccccgccg atggctgaaa gcagtacgcc ccaggtccaa ccgtagattc caccctctcc
     1261 ggcatagata aggtagtaga gcaagccata caataccaag ccgatgagga tggccaacaa
     1321 aacgaaacgc gctgctgcac ccgtgtcgcg ccggagctgg agatagtggt agcggcgggc
     1381 gtgctcgttg gggaaaaaac tctttttgcg gggcaggcgg ggcgctgtgc cgactgtctg
     1441 cgaaacgggg gcgctgtatt gcacggcggc ggaaagcact acgggcgaca gcgtgatggc
     1501 aagagcaagt agggcgaaac ggagggtcat tgtgattctt tatgccctct aacgctttgc
     1561 gctttgcctg acttttttga cggcgtcttt ttaacgcccc aaattagggc gatgaaaaat
     1621 aaatcttcat tttaagtgcc ttaactaatc ctttgaccta aagaaaatga acatttattt
     1681 ttgccccact acgttgcgga tgagcggtgc atcgcgttcc agatcgaagg cgcggccatt
     1741 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnngcttggt catttgcagt tcggtgtagc cctggcggag gagttggagc
     1921 aggtctaagt tttgggcggc ggccatgtcg ggtattactt tggagtaggt tttgccgggt
     1981 gtgagcctca tgccgcgggc gtccacctcg tcgaaggcat gaaaaatgga gaggtcgccc
     2041 tggtcggaga tggacacgct ggaaccgtcg cggcggaagc cgatgtcgag gnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnagg cggcggagtt cttgtttttc gggatcgttg
     2281 ggcagcacgc ccgcctcgat gaggatggtg gaggtgcccc atttttggat gttgtcgccg
     2341 aaggcgcgcg gctcgaactc gtcgtcgtag cgggcgatgt ggccggggat gtactcatgg
     2401 aggaaggcgc tgaggccggc gatgagttgc atggcattgc cgcggctgtc gttgatttct
     2461 ttttcgtaat tgtaggccgg agcgaggaag gccatggctg catttttggg accgaggccg
     2521 gcgccgtaga agcggccctg atcgtggagg ttgaagcccc attgggcgga gagttcgtcg
     2581 cggaggcgct tgagcagttg agattcaggg cattgcaggc ggagggcatc gcgattgagg
     2641 tctatgccga gggcattgcg gcgttcgaag cgctcggcgc cgtcggggtt gagcatgggt
     2701 acaaagacga gggtgagttc gctgcggagg cggctgcgga gttcgtccca ttcgtcggag
     2761 cggctgagga agttgaagat gtccatgagg gccatggtgg cggtgggttc gtcgccgtgc
     2821 atttgcgacc aaagcagcac cttgatgggg ccgttgccgt actgcacacg ataaatattg
     2881 cgcccctcga tggacttgcc gtctacgctc cactggaagg gcgactggag gcgctgcacc
     2941 aagggcgcaa tggcggcgtg cttgaagcgc tggcgggtga tgctgggttc tttgtaggcg
     3001 ctgtggcgat cgtagagggc tttgtacatg gaaaaatcgg cgccgtcggt gggtgcagcg
     3061 ctttggtggg cggcatggcg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
     3121 tggcgtcggc gggacggcag gagcagaggc cggcgacgag agcaaagaca aagaagtggc
     3181 gcatgaattg gatttttgcc gtgcaagtta ggcgtatgta ttggttttgc tatcttcgcc
     3241 cggcaagttt cacctttata aaaatgattc tttgacaaat agtatgctta aaggaacaga
     3301 tttagcgaaa atcatgcctc cttacagtcg tcctgcc
//
LOCUS       DAORLF010000487         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15730_length_3314_cov_1.959804, whole genome shotgun sequence.
ACCESSION   DAORLF010000487 DAORLF010000000
VERSION     DAORLF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15730_length_3314_cov_1.959804"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..273
                     /locus_tag="PK198_24450"
     CDS             139..273
                     /locus_tag="PK198_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41975.1"
                     /translation="MSMTKRKRRLEAKDHNAARKFYLIVGIATVLLMLMLYLVYRSAS
                     "
     gene            complement(221..>894)
                     /locus_tag="PK198_24455"
     CDS             complement(221..>894)
                     /locus_tag="PK198_24455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRF41976.1"
                     /translation="PRLHIVDRYLAAAAPLGVQNDGLGLDYFIPETDEEAAQDFLAAH
                     FGGGVPVFIALVTGAAHATKRLPEARLIELCRLMTMPVVLLGGPDEQEQGERIAAQGG
                     AHVVNACGKLKLNQSAGVLRRAQTVVTHDTGLMHIAAALGKDIVSIWGNTVPEFGMTP
                     YIPGGDASGKILEVKGLSCRPCSKIGHQACPKGHFRCMNDIDFSEIINWRSDTPDRAS
                     ASTIP"
     assembly_gap    895..1103
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1313..>3314)
                     /gene="carB"
                     /locus_tag="PK198_24460"
     CDS             complement(1313..>3314)
                     /gene="carB"
                     /locus_tag="PK198_24460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRF41977.1"
                     /translation="GGCNIQFAVDPDTEDMIVIEMNPRVSRSSALASKATGYPIAKIA
                     AKLAIGYRLDELNNQITKNTSAFFEPTLDYVIVKMPRWNFDKFPGADHRLGLQMKSVG
                     EVMAIGRNFSEALQKACQSLENNRTGLGADMKEWIRTSDVLERLEQVSDDRIFRVKDA
                     LRLGVPEKTIQKLTKIDPWFIKQIKRLVKMEERLLRYNVAEDIPEDFFRELKESGYSD
                     AQIAWLLRIKEEEVFRRRIELGIRRTFKMVDTCAAEFEAQTPYFYSTFDHENESIPSD
                     KKKIVVLGSGPNRIGQGIEFDYCCVHGLMAIHEAGYESIMINCNPETVSTDFNIADKL
                     YFEPVYWEHIEEILELEKPEGVIVQLGGQTALKLAETFYKKGIPIIGTSYANMDLAED
                     RGAFSNLLHELEIPYPRYGVAIDVDEAIRIANEVTYPVLVRPSYVLGGQNMRIVINDT
                     ELENHVLNIFKHLPDNRVLIDQFLDRAKEAEIDCICDGEDVHIMGIMEHIEPAGIHSG
                     DSSAVLPTYSLSVQAVATMVEYAEKLAKALQIKGLLNVQFAIKTSKVPGEPDKVYVIE
                     ANPRASRTTPFIAKAYKVPYLQIATQVMLGVKKLRDFNIQPQPSGFAIKVPVFSFEKF
                     PNVDISLGPEMKSTGEAIRFIKDLSDPFFRDLDSKRNMYLTR"
BASE COUNT          677 a          856 c          832 g          740 t
ORIGIN      
        1 cataaagatc agcgtcaaaa aaacagcagg cctccgacgc cgaaagacac tgatttttat
       61 accgcccaaa gacattcaac ctgtcgaagg ataatgtata tttgcaggca atactctcca
      121 ctcaaaaatg caggaaacat gagcatgacg aaacgcaaaa gaaggctgga agccaaagac
      181 cacaacgccg cccgcaaatt ctacctgatc gtcggcattg ctacggtatt gttgatgctg
      241 atgctctatc tggtgtatcg gagcgccagt tgatgatttc agaaaagtca atgtcgttca
      301 tacatctgaa gtgtcctttg gggcaagcct gatgcccgat cttggagcaa ggccggcatg
      361 ataatccttt cacttccaat attttacccg acgcgtctcc accgggaatg taaggcgtca
      421 tgccgaactc cggcacggta tttccccaaa tcgaaacaat atccttgccc aatgccgccg
      481 ctatgtgcat cagtccggta tcgtgggtga cgaccgtttg cgcccggcgc aacactcccg
      541 ccgactggtt cagtttcaat tttccgcaag cattcacaac gtgcgcgccg ccctgagccg
      601 caatacgttc gccctgctcc tgctcatcgg gaccgccaag caagactacc ggcatggtca
      661 tcagccggca cagttcgatc agccgcgcct ccgggagtct tttggtggca tgtgccgcgc
      721 ccgttaccaa agcaatgaaa acaggtacgc ctccgccgaa atgcgcagcc agaaagtctt
      781 gagccgcctc ctcatcggtt tcaggaatga aataatccaa gcccaggccg tcgttttgca
      841 cgcccaaagg cgcagcagcg gccagatagc ggtccacaat gtgcaggcgg ggcannnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnccaccac gctgcgaaaa ttttctttgg tcagaaaatg
     1141 cagctcggcg cccgtttgca tacggatgca gcgcagcacc ggcgttgtca gcacgatgtc
     1201 gccgatggaa gaaaaacgaa tgatgagtac tttgggcatc ctatgactat tcagtgtctc
     1261 tgcgagttac gaaacacaac gccacgctcg ctttgcaact cgcagctcgc ggctatctgg
     1321 tcagatacat attccgcttg ctgtccaaat cccggaaaaa cggatccgac aaatctttga
     1381 tgaatcggat ggcctcgccg gtggacttca tctccggccc cagactgatg tccacgttgg
     1441 ggaatttttc aaacgaaaac accggcacct tgatggcaaa accgctgggc tgcggctgta
     1501 tgttgaaatc gcgcagtttt tttacaccca acatcacctg cgtggcaatt tgcaggtaag
     1561 gcactttgta ggctttggct ataaacggcg tggtgcgcga ggccctcgga ttggcctcaa
     1621 tgacatacac cttgtcgggt tcgccgggta ctttggaggt tttgatggca aactgcacat
     1681 tcagcaaacc tttgatttgc agggctttcg ccaatttctc cgcgtattcc accatcgtag
     1741 ctactgcctg cacagagagg ctgtaagtgg gcagtaccgc cgagctgtcg ccggagtgaa
     1801 tgcccgccgg ctcgatgtgc tccatgatgc ccatgatgtg tacgtcctcg ccgtcgcaga
     1861 tgcagtctat ttcggcctct ttggcgcggt cgaggaactg gtctatcaac acgcggttgt
     1921 cgggcagatg tttgaaaata ttaagtacat ggttctccaa ttctgtgtcg ttgatgacga
     1981 tgcgcatgtt ctggccgccc aacacataag aaggtcgcac caacaccggg tacgtcacct
     2041 cgttggcaat gcggatggcc tcgtccacgt cgatagccac gccgtagcgc gggtacggaa
     2101 tttcgagttc gtgcaggaga ttggaaaacg cgccccggtc ttcggccaag tccatattgg
     2161 cataagaagt gccgatgatg ggaatgcctt ttttgtagaa agtttcggcg agtttcagcg
     2221 ccgtttggcc gccgagctgc acaatgacgc cttcgggttt ttccaattcc agaatttcct
     2281 caatatgctc ccaatacacc ggctcgaaat agagcttgtc ggcgatgttg aagtcggtag
     2341 aaaccgtctc cgggttgcag ttgatcatga tgctctcgta gccggcctcg tgtatggcca
     2401 tcagcccgtg tacgcagcaa tagtcgaact cgatgccctg cccgatgcgg ttgggaccgg
     2461 aacccagcac cacgattttc tttttgtcgc tgggaatgct ctcgttttcg tggtcgaaag
     2521 tggagtaaaa atagggcgtt tgggcctcaa attcggcggc gcaggtgtcc accattttga
     2581 acgtgcgccg gatgccgagt tctatgcggc ggcggaacac ctcctcctct ttgatgcgca
     2641 gcagccaggc gatttgcgcg tcggagtagc ccgattcttt cagttcgcgg aaaaaatctt
     2701 ccggtatgtc ttcggccacg ttgtagcgca gcagtcgttc ctccattttc actaaacgct
     2761 tgatttgttt gatgaaccag gggtctattt tggtcagttt ctggatggtt ttttccggca
     2821 cgccgagccg cagcgcatcc ttgacgcgga aaatgcggtc gtcgctcacc tgctccaagc
     2881 gctccagcac atcggaggtg cgaatccatt ctttcatgtc ggcgccgagg ccggtgcggt
     2941 tgttttccaa tgactggcaa gccttttgga gcgcctcgga gaaattgcgc ccgatggcca
     3001 tcacctcgcc caccgacttc atttgcaggc cgaggcggtg gtcggcgccg gggaatttgt
     3061 cgaaattcca gcgcggcatt ttcacgatca cataatcgag cgtgggttca aaaaaggccg
     3121 aagtgttttt tgtgatctgg ttgttgagtt cgtccaaacg gtagccgatg gcgagtttgg
     3181 cggctatttt ggcaatcggg tagcccgtcg ccttgctggc caaagccgag gagcgcgaca
     3241 cccgcgggtt catctcaatg acgatcatgt cttccgtatc ggggtccacg gcaaactgta
     3301 tgttgcagcc gccc
//
LOCUS       DAORLF010000488         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15733_length_3314_cov_1.725069, whole genome shotgun sequence.
ACCESSION   DAORLF010000488 DAORLF010000000
VERSION     DAORLF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15733_length_3314_cov_1.725069"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>254
                     /locus_tag="PK198_24465"
     CDS             <1..>254
                     /locus_tag="PK198_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41978.1"
                     /translation="RGHARGLLSLLEGYYFEPDTFAIPAYPAAMIAMPNTAPARDLPD
                     APLLFPNPAQDRLSVQIPSTWKGSGRIIVTDMPGRTIMERP"
     assembly_gap    255..336
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            520..>1694
                     /locus_tag="PK198_24470"
     CDS             520..>1694
                     /locus_tag="PK198_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41979.1"
                     /translation="MRSIDMNNEGDLGLQIYKVEDQYMISVGSLINNIKCLNLLRVDA
                     EGNVIYKRQYSSLPYNWEPNAYTMCRVGDRSYIAGQIYDPQNNTQGFIMEFDDDTGDS
                     LGMIIQGGPYQELVINLTPTFDGNLLMGAYRHFSDNRIWLQKRRPDLSLVWERFLGPS
                     FSWQGLGKIVESVDGEIVFGRRVCLHPSGLCTKENLLITRTDSTGQEIWSIMHPEKAD
                     NTHPSMVAMDNGEVAVSWARDLWEELDSMIPYPPSVLWYSREGELVRRYDFASTVGRD
                     IFNLKIASNGDIFGVGMGDRRAESMGLTGWLFRLSPQGELLWERYISDIRNSVKLQYF
                     RDLHEEPDGSLVLVGSHNDTFPDHIPFVNNPNVWFVRLDSTGCFEPGCGSIQIITSA"
     assembly_gap    1695..1719
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1720..1981
                     /locus_tag="PK198_24475"
     CDS             <1720..1981
                     /locus_tag="PK198_24475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF41980.1"
                     /translation="MPSGSPELILFPNPAGASFSLLMEGYSQPVYPAQAKMWDMQGRL
                     LEERTVGAPSEIWYCEDRPPGVYWIQIHTSAGQAGVAKLVKR"
     gene            complement(1988..>2313)
                     /locus_tag="PK198_24480"
     CDS             complement(1988..>2313)
                     /locus_tag="PK198_24480"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRF41981.1"
                     /translation="LEAMEADSGIRIAELRVDGGATVNNFLMQFQCDVLDCRVVRPQV
                     HETTALGAAYLAGLAVGYWQNLAEIGQQWQADRSFSPTMSDAQRNELKKGWQKAVKGV
                     IAVAE"
     assembly_gap    2314..2413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2414..>3314)
                     /gene="glpK"
                     /locus_tag="PK198_24485"
     CDS             complement(<2414..>3314)
                     /gene="glpK"
                     /locus_tag="PK198_24485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRF41982.1"
                     /translation="LAVCREALAQAGAGAGEVAAIGITNQRETAVVWNRSTGRPIHHA
                     IVWQDRRTAGFCDELKAQGKSALIQQKTGLVIDAYFSATKVRWILDHVPGARALAEQG
                     ELCFGTIDTWLLWKLTNGKVHATDVSNASRTMLCNIHTLEWDPELLELFGVPAAMLPQ
                     IRSSSEVYGHTENVLTATRMPIAGIAGDQQAALFGQMCIEPGMVKNTYGTGCFMLMNT
                     GTQAVASQNALLTTVAWQIDGLTHYALEGSVFIAGAVVQWLRDGLQIIRSSAEVEALA
                     LEVDSTDGVYVVPAFAGLGAPHWN"
BASE COUNT          695 a          841 c          823 g          748 t
ORIGIN      
        1 cgcggtcatg ccagggggtt actttctttg ttggagggct attattttga accggatacc
       61 tttgctattc cggcctaccc tgcggccatg attgccatgc ccaatacggc gcctgctcgc
      121 gacttgccgg atgcgccttt actctttccc aatccggcac aagaccggct atcggttcaa
      181 attccgagta catggaaggg ctccgggcgt atcatagtca ccgatatgcc ggggcgtacc
      241 atcatggaac gcccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga caggccctct tccacggcaa
      361 aatcgtaaag caataacctg catttcctca catcgggagc ggcggtggct cgaaagtcat
      421 ggccgctccc tcacttcatt tttactaatg aataagctca tataccttac cggcatactg
      481 ctatgcctgt gtttttatac atcagcccag caaactttca tgcgttccat agacatgaat
      541 aacgaaggag accttggcct tcagatatat aaagtggaag accaatatat gatttcggtc
      601 ggctctctga tcaacaatat caaatgcctg aatttattaa gagtggacgc agagggcaac
      661 gtcatttaca aaagacaata cagctcgctg ccttacaatt gggaacctaa tgcttatact
      721 atgtgccggg taggcgacag aagctatatt gcagggcaga tttatgatcc ccagaacaat
      781 acacaaggct tcatcatgga gtttgatgat gacaccggcg atagtttggg aatgatcatt
      841 caaggtgggc catatcagga gttggttatc aatctgaccc ccacgtttga cggcaacctg
      901 ctcatgggcg cgtatcgcca tttttcagac aacagaatct ggttgcaaaa acgcagaccc
      961 gatttgtctt tggtatggga acgatttttg gggccttctt tcagttggca gggtttggga
     1021 aaaatagttg aatcagttga cggagaaatt gttttcggac gccgggtttg cctgcaccca
     1081 tccgggctgt gtaccaaaga aaatctgctg attacccgta cagactctac aggacaagaa
     1141 atatggtcca tcatgcaccc tgaaaaagcg gataacacac atccctctat ggtggcaatg
     1201 gacaatggag aggtggctgt ttcatgggca agagacttat gggaggaatt ggacagtatg
     1261 atcccatatc cgccttctgt actttggtat agcagagagg gcgagcttgt acggcgatat
     1321 gattttgcat ccacggtggg cagggatatt tttaatctca aaatagcttc taacggggat
     1381 atatttggag tcggcatggg ggatagaaga gcagaaagca tgggcttaac gggctggttg
     1441 tttagattat cccctcaggg cgaattactc tgggaacggt atatatccga tattcgcaat
     1501 tccgttaagt tgcagtattt tcgcgacctg catgaagaac cagacggaag tttggttctt
     1561 gtcggaagcc ataatgacac ttttcctgat catatccctt ttgtcaacaa cccaaatgta
     1621 tggtttgtgc gcttagatag taccggctgc ttcgaaccgg gctgtggctc catacaaatc
     1681 atcacttctg cacannnnnn nnnnnnnnnn nnnnnnnnna atgccctcag gcagccctga
     1741 acttatcttg tttcccaatc ccgccggcgc ttctttttcg ctacttatgg agggctattc
     1801 ccaacccgtg tatcctgcgc aggccaaaat gtgggacatg cagggccgcc tgctggaaga
     1861 acgcaccgtg ggtgcgccgt ccgaaatctg gtactgcgaa gaccgcccgc caggcgtata
     1921 ttggatacag atacacacct ccgcagggca ggcaggcgtg gccaagttgg tgaagcggta
     1981 accaacctca ctccgccacc gctatcaccc ccttcaccgc tttctgccag ccttttttta
     2041 gttcattgcg ctgtgcatcg ctcatggtgg gcgagaagct gcggtcggcc tgccattgct
     2101 gcccgatttc ggctaagttt tgccaatagc ccacggccag gcccgccaga taagcggcgc
     2161 ccagcgcggt agtttcgtgt acctgtgggc gcaccactct acaatccagc acatcgcatt
     2221 ggaactgcat cagaaaattg ttgaccgtag cgccgccgtc cacccgcaat tctgcaatcc
     2281 gaatgcccga atcggcctcc atcgcttcca gcannnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnattccag tgcggcgcgc ccaatcccgc gaaagccggc accacataca
     2461 ccccgtcggt agagtccact tcgagggcca aagcctccac ctcggcggaa gagcgaatga
     2521 tttgcaggcc gtcgcggagc cattgcacta ccgccccggc aatgaacacg ctgccttcga
     2581 gtgcgtaatg tgtgaggccg tcaatctgcc aggctacggt ggtgagcagt gcattttggc
     2641 tggccactgc ctgcgtgccc gtattcatca gcataaaaca gcccgtgccg taggtgtttt
     2701 tcaccatgcc cggctcaatg cacatttgtc cgaagagcgc cgcctgctgg tcgccggcaa
     2761 tacctgcaat gggcatgcgg gtggcagtga gcacgttttc cgtgtgacca tatacctcgc
     2821 tgctgctgcg gatttggggt agcatagccg ccgggacgcc gaacaattcc agcaattcgg
     2881 ggtcccattc gagggtatgg atattgcaga gcatggtgcg ggaggcgttg ctcacatcgg
     2941 tggcgtgtac cttgccgttg gtgagtttcc acagcagcca ggtgtcaatg gtgccgaaac
     3001 aaagttcgcc ctgttcggcc aatgcgcggg cgccgggtac atgatccaat atccaccgca
     3061 cttttgtggc gctgaaatag gcgtctatta cgaggccggt tttttgctga atgagcgcgc
     3121 ttttgccctg tgctttcagt tcgtcgcaga agccggcggt gcggcggtct tgccacacaa
     3181 tggcatgatg gatgggccgt ccggtgcttc gattccacac caccgccgtt tcgcgctgat
     3241 tggtgatgcc gatggcggca acctcgccgg cccctgcccc ggcctgggcg agcgcctcgc
     3301 gacaaaccgc cagc
//
LOCUS       DAORLF010000489         3312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15744_length_3312_cov_2.020878, whole genome shotgun sequence.
ACCESSION   DAORLF010000489 DAORLF010000000
VERSION     DAORLF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15744_length_3312_cov_2.020878"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    646..730
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            806..>1025
                     /locus_tag="PK198_24490"
     CDS             806..>1025
                     /locus_tag="PK198_24490"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF41983.1"
                     /translation="MDGFRWDYLDRFQPPRLSQFVKEGVRAESMLSCFPSKTFTNHYS
                     IATGMYPNHHGLVDNNFFDADKNLVYGMG"
     assembly_gap    1026..1160
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1161..1949
                     /locus_tag="PK198_24495"
     CDS             <1161..1949
                     /locus_tag="PK198_24495"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF41984.1"
                     /translation="RPHYYYNYDGSISNEKRVQQALDWLRLPEAERPRLITLYFSDMD
                     DQGHRYGPNADEKLREKLMALDTALGQLFDGLNTIQLPVNVLFVSDHGMAEISVKNLL
                     PIEPIEDEAQYLTVNSGALVHLYLRDTTAAETVYQNIKAAENHYKIYRTAETPYFETP
                     PTNPRWGDFIVVPDSGYYFVNARTMGFRQRSGDVVMGEHGFDPERREMHGIFFAGGPA
                     IQQGKVVPSFKNIHVYPLMCRILGIGIPPDVDGKAEVLAPVLRR"
     gene            complement(1991..3061)
                     /locus_tag="PK198_24500"
     CDS             complement(1991..3061)
                     /locus_tag="PK198_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41985.1"
                     /translation="MIPSKKKLPYRVGEPLLRYLKRYNRARQLPLQYEDLTRYESSAP
                     LIDHTGKDTLWETVYFSESDRPDVFHALTSIYALLKTDGDMGVVEHLTVARIDYCTFG
                     NTKPFRVRIINRMNDNYDHFYVKRADASRIYGLELEELLSPNSVMYLVDGGTMIEEHI
                     VGIPGDDFIRLHLDDEPFNQVRIAKEFVKFNERCFVRLLGDMRSYNYVVAVTPDIEGA
                     QFRIRAIDFDQQSYEGRKSFYLPQYFKENNPIIQLGIKHLSQESVNQYQSEERSLIAA
                     RAKATRSRLDALFQCMTEDEISSQEKVRLLAEDLARHHDNKAFLACKSMGEIVLTNIN
                     LLLQKDFRQGIPGGREIAAGKY"
BASE COUNT          718 a          842 c          815 g          717 t
ORIGIN      
        1 gattgcaggc acccaaaatg cggtacggac ggaagtcttt gtctatcttc tttttgaacg
       61 tttcctgcaa gtcaatttgc gtgacaatgc cgaagccctt ggccttcagt gcatcggtag
      121 tgcgttgcac ggcagtgtcg aaatcggatt caatagtacg gctgaaatag taactcatgg
      181 tattgttgat tttgggccgc aagttgcgct gccggaaggc aaacggacag tgagggaggt
      241 cacataggag aagctgcgcg ttccgggttt cgggttgcat ccgccgtagc cttagcgaag
      301 gcgggttcaa ggtttcgggt tgcatccgcc gtagccttag cgacggcggc gtggttcgcg
      361 gaaattcgcg cccttcgcta aaattcgcgt taaaaaaact atgtttagcg ggtggaaccc
      421 gcatgatagc cggcaccacg ccgggcgtgg taaacatgaa ggtgtgaacc tcgcgccggc
      481 aggtttcgcg tttccctcgc attcgcggaa tttcgcgccc ttcgctacaa ttcgcgttaa
      541 aaaaattcta tgtttagcgg gtggaacccg catgatagcc ggcacgaggt gtgaacctcg
      601 cgccggcagc gcagtgaggc aggtcacttt gagtgctttt ttacgnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn tccgcctgtc aacgcagtct ctcctccgcc gagaaacccg ccgccgccca
      781 agctcaatac ctcatcctga tttccatgga cggctttcgc tgggattact tagatcgctt
      841 ccagccgccc cgcctcagcc agtttgtcaa agagggcgtt cgggcagaat ctatgctctc
      901 ctgctttccc agcaaaacct tcaccaacca ttactccatc gccaccggca tgtaccccaa
      961 tcatcatggc ttggtggaca acaatttttt cgatgccgac aaaaatttag tctatggcat
     1021 gggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn cggccgcatt attactacaa ttatgacggc agtatttcca
     1201 acgaaaaacg agtgcaacag gcgctggact ggctgcggct gcccgaagcg gaacgccccc
     1261 ggctcatcac cctgtatttt tcggatatgg acgaccaagg ccaccgctac ggcccgaacg
     1321 cagatgaaaa acttcgcgaa aaattgatgg cattagatac ggcgctgggg caactctttg
     1381 acggactcaa caccatacaa ctacctgtaa atgtgctttt tgtctccgat cacggcatgg
     1441 ccgaaatttc tgtaaaaaac ctcctcccca ttgaacccat cgaagacgag gcgcaatatc
     1501 tcaccgtcaa cagcggcgcg ctggttcacc tctacctgcg cgatacgacc gccgcggaaa
     1561 cggtgtatca aaacatcaaa gccgccgaaa atcattacaa aatctatcgc actgccgaaa
     1621 cgccctattt tgaaacgccg cctacgaacc ctcgatgggg cgacttcatc gtcgtgccgg
     1681 attcggggta ttatttcgtc aatgcgcgta ccatggggtt ccggcagcgc tccggagatg
     1741 tggtaatggg cgaacacggt ttcgaccccg aacgccgcga aatgcacggt attttcttcg
     1801 caggcgggcc ggccattcag cagggcaaag tagtgccgag tttcaaaaac atccacgttt
     1861 accccctgat gtgccgcatc ttaggcatcg gcatcccccc cgatgtggac ggcaaagcgg
     1921 aggtgttggc gccggtctta cgccgctgag acaacccatc agcacattct cacatcagca
     1981 cattctcaca tcaatacttc cccgcagcaa tctcccgtcc tccgggtatc ccctgccgga
     2041 agtccttttg tagcagcagg ttgatgttgg tcagcacgat ctcgcccatg cttttgcaag
     2101 ccaggaacgc tttattatcg tggtggcggg ccaggtcttc ggctaagagc cgtacttttt
     2161 cctgcgatga aatttcgtct tccgtcatgc actgaaacag cgcatccaag cggctccggg
     2221 tggctttggc acgggcggcg atgaggctgc gctcttccga ctggtactga ttgacgcttt
     2281 cctgactgag gtgtttgata ccgagttgaa tgatggggtt gttttctttg aaatattgag
     2341 gcagataaaa actcttgcgc ccttcgtaag attgctgatc gaaatcaatg gcgcgaatgc
     2401 gaaactgcgc gccctcaatg tcgggcgtga cggccaccac ataattgtag gaacgcatat
     2461 cgcccagcag ccgcacaaag cagcgctcgt tgaatttgac aaactccttg gcgatgcgca
     2521 cctgattgaa cggctcgtcg tctaagtgca gacgaatgaa atcatcgccg ggaatgccca
     2581 cgatgtgctc ttcgatcatg gtgccgccat ccaccagata catcacgctg ttgggcgaga
     2641 gcagttcttc caattctaag ccgtaaatgc gggaagcatc ggcgcgtttg acgtagaagt
     2701 ggtcgtagtt gtcgttcatc cggttgatga tgcgcactcg gaaaggcttg gtattgccga
     2761 aggtgcaata gtctatgcgc gccacggtga ggtgctccac cacgcccata tcgccgtcgg
     2821 ttttcagcag cgcgtatatc gaggtgaggg catggaacac atccgggcgg tcggattctg
     2881 aaaaatagac cgtttcccag agggtgtcct tgccggtatg atctatcagc ggtgccgagg
     2941 attcatagcg ggtgaggtct tcatattgca gcggcaattg ccgggcgcgg ttgtagcgtt
     3001 tgaggtagcg cagcagcggt tcgcccacgc ggtacggaag tttctttttg gacgggatca
     3061 ttgtacgatc atttctttag aaatactttt tgggccgcaa gccccacgct gcaagttagg
     3121 aagaaactga aaaatctccc accgttttca tacaacacct taaactttga acccgaaacc
     3181 cgttcctacc cgtcaatccg aaatccgaaa tccgaaatcc gaaatcagga atcaggaatc
     3241 ggaaatccga aatcaggaat caggaatcag gaatcggaaa tccgaaatca ggaatcagga
     3301 atcaggaatc gg
//
LOCUS       DAORLF010000490         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15796_length_3304_cov_1.554632, whole genome shotgun sequence.
ACCESSION   DAORLF010000490 DAORLF010000000
VERSION     DAORLF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_3304_cov_1.554632"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PK198_24505"
     CDS             <1..1082
                     /locus_tag="PK198_24505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRF41986.1"
                     /translation="DLLRDTILVVVAQLIGVDSKEVIFTSGATESDNLAIKGVFEMYR
                     RKGNHIITVKTEHKAVLDTCDHLEKLGAEITYLDVQADGLIDLAALEAAIKPTTILVS
                     VMWANNETGVIQPMAEIGKICEKHGVLLMSDATQAVGKIPVMPKEAGVHLLAFTSHKM
                     YGPKGVGALYVSRKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAEIARLEMA
                     QDAERLSRLRDKLENSMKEMEEVYVNGNVDHRMPHVCNISFKHVEGEGLMMTFNQNIA
                     VSSGSACTSASLEPSYVLVALGLGDDLAHSSIRFSLGRFTTEEEIDQAIEMVKKGVNH
                     MRDLSPIWEMYKEGVDLSTVEWSEH"
     gene            1184..>1348
                     /locus_tag="PK198_24510"
     CDS             1184..>1348
                     /locus_tag="PK198_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRF41987.1"
                     /translation="MAYSQKVLDHFQNPKNVGTLDKSSKNVGTGLVGAPECGDVMRLQ
                     IEVDPETQVIT"
     assembly_gap    1349..1543
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1635..1961
                     /locus_tag="PK198_24515"
     CDS             1635..1961
                     /locus_tag="PK198_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF41988.1"
                     /translation="MLFVADSAKTKIQEIKAKEHIGDDYFVRVSITSGGCSGLTYKMD
                     FDNESKPNDQVFEDNGVKVVTDLKSFLYLFNSTLEFSGGLEGKGFSFNNPNAARTCGC
                     GESFAV"
     gene            2068..>2355
                     /locus_tag="PK198_24520"
     CDS             2068..>2355
                     /locus_tag="PK198_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF41989.1"
                     /translation="MFRIGYDAKRLFNNFTGLGNYSRTLLQNMATYYPDNEYFLYTPK
                     VNKSPETAFFLNSPLFSVHTPRRGSGAWWRSFSVVNDLKRHKIQLFHGLSHE"
     assembly_gap    2356..2458
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2459..3218
                     /locus_tag="PK198_24525"
     CDS             <2459..3218
                     /locus_tag="PK198_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF41990.1"
                     /translation="DRHIYDIKFRHACQQADRVIAISESTRNDIIEFYQIAPEKISVI
                     YQSCHERFQQEKSQKTIDYVLKKYQLPTDYLLFVGSLSERKNPLAVLQAMLQLPSAIQ
                     LPLVIIGEGQAYKKKVMEFVQQHKMERKVLFIRPEYEDMPAIYQQADVFLYPSFYEGF
                     GIPILEALFSETPVITSNVSSLPEAAGPDSWLVHPDKPEEMAAGIQKILEDQAFRRNM
                     IEKGYQYAQRFQGDVLSDQIMELYEDVLKIDAIV"
BASE COUNT          804 a          740 c          762 g          700 t
ORIGIN      
        1 acgacctgct gcgcgacacc atcctggtgg tggtggcgca gctcatcggc gtggattcca
       61 aagaagtcat ttttacctcc ggcgccaccg aatcggacaa cttagccatc aagggcgtat
      121 ttgaaatgta tcgtcgtaaa ggcaaccaca tcatcacggt gaaaaccgag cacaaagccg
      181 tcctcgatac ctgcgaccac ttggaaaaat tgggcgccga aatcacttat ttagacgttc
      241 aagccgatgg tctgattgac ctcgccgcat tggaagcagc catcaaacct accaccatcc
      301 tcgtgtcggt gatgtgggcc aacaacgaaa cgggcgtcat tcagcctatg gccgaaatcg
      361 gtaaaatttg cgaaaagcac ggcgttttgc tcatgagcga cgccacgcag gcagtgggta
      421 aaattccggt aatgcccaaa gaagccggcg ttcacctgct ggcattcact tcgcacaaaa
      481 tgtacggccc caaaggcgtg ggcgctctgt atgtgagccg caaaaatccg cgcgtgaagg
      541 tgaccgccca aatggacggc ggcggtcacg aacggggaat gcgctctggt acgctcaacg
      601 tgcccggtat tgtgggcttc ggcaaagccg ctgaaatagc ccgcctcgaa atggcgcagg
      661 atgccgagcg cctcagccgc ctccgcgaca agcttgaaaa ctctatgaag gaaatggaag
      721 aggtgtatgt gaacggcaac gttgaccacc gcatgccgca tgtgtgcaac atttcgttca
      781 agcatgtaga gggcgagggt ttaatgatga cgttcaacca aaacatcgcc gtgtcttccg
      841 gttcggcttg tacctcggcg tctttggagc cttcttatgt attggtggct ctgggtctcg
      901 gcgacgatct ggcgcactct tccattcgtt tcagcctagg tcgtttcacc acagaagagg
      961 aaattgatca ggccatcgaa atggtcaaaa agggcgtcaa tcacatgcgc gacctcagcc
     1021 cgatttggga aatgtacaaa gagggtgtgg acttgagcac ggtggagtgg tcggagcatt
     1081 aattttcggg gtgggaacgg cggcaagctg caaattacaa gctacaagtg tttgacaatc
     1141 agtatttttc agatagttga caaccaataa aaataattaa acaatggcat attctcaaaa
     1201 agtactcgat cattttcaga acccgaaaaa cgtgggcaca ctcgataaga gcagcaaaaa
     1261 cgtaggtacg ggtttggtgg gcgctcctga atgcggcgac gtgatgcgcc tgcaaattga
     1321 ggtggacccg gaaacgcagg tcatcaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatcaaa agcgccatca
     1561 aagattacaa agagaaaaac ggcatttcgg tagaagctga agcggctgtt cactaagtac
     1621 taattcattg caccatgctt tttgtagcag acagcgccaa gaccaagata caggaaatca
     1681 aggcgaaaga acacattggc gacgactatt tcgttcgggt gagcatcacc agcggaggct
     1741 gttcgggcct gacgtacaaa atggatttcg acaacgaatc gaagcccaac gatcaggttt
     1801 ttgaagataa cggcgtcaag gtggtcactg atctgaagag ctttctgtac ttgttcaact
     1861 ccacgctgga gttttccggc ggcctcgaag gcaagggctt ttcattcaat aaccccaatg
     1921 cagcccgcac ctgcggctgt ggggagagct ttgcagtata ggggatttaa gttgctgaaa
     1981 cataagttct gaaaaaactc cgatgcgggt gcatacttcg cattggagtt tttctttacc
     2041 ttcacttcag taagcaacca acatctcatg ttccgcatcg gctacgacgc caaacggctt
     2101 ttcaacaact tcaccgggct gggcaattac agtcgcacct tgctccaaaa catggctact
     2161 tattacccgg acaacgagta ttttctctat acgccgaagg taaacaaaag ccctgaaacc
     2221 gctttttttc tcaatagccc gctctttagc gtgcacacgc cccggcgggg ttcgggcgca
     2281 tggtggcgca gtttctcggt agtcaatgat ttgaaacgcc ataaaattca gttgtttcac
     2341 ggactgagcc acgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     2461 accgtcatat ttacgacatc aaattccgcc atgcctgtca acaggccgac cgcgtcattg
     2521 ccatcagcga aagcacccgc aacgacatca tagaatttta ccagattgca cccgaaaaaa
     2581 tcagcgtcat ctatcagtct tgtcacgagc gattccaaca ggaaaaatct caaaagacga
     2641 ttgattacgt tctgaaaaaa taccagctac ccactgatta tctgctgttt gtgggttcct
     2701 tgagcgagcg aaagaacccg ctggcggttt tgcaagccat gctccaattg ccttctgcca
     2761 ttcagttgcc tttggtcatc atcggcgaag gacaggctta taaaaagaag gtgatggagt
     2821 ttgtgcagca acacaaaatg gagcgcaagg ttttgtttat cagaccggaa tacgaagaca
     2881 tgccggccat ttaccagcag gccgatgtat ttttataccc ctctttttat gaaggtttcg
     2941 ggattccgat tctggaagcg cttttcagcg agacgcccgt cattacgtct aatgtatcct
     3001 cgttgcccga agctgccggc cccgattcct ggctcgtaca ccccgacaag ccggaggaga
     3061 tggccgccgg tattcagaag attttggagg atcaggcctt ccggcgcaat atgattgaaa
     3121 aagggtacca gtacgcacag cgttttcagg gcgacgtgtt gtctgaccaa ataatggagc
     3181 tttatgaaga cgtactgaaa atagatgcga ttgtgtaagg ggcgataaaa aataaagtgg
     3241 tcattttcgc caaaggatta gatgagtaaa cgtttcattt taagtgtctt atctaatcct
     3301 ttgg
//
LOCUS       DAORLF010000491         3283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15932_length_3283_cov_1.833953, whole genome shotgun sequence.
ACCESSION   DAORLF010000491 DAORLF010000000
VERSION     DAORLF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15932_length_3283_cov_1.833953"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>214)
                     /locus_tag="PK198_24530"
     CDS             complement(<1..>214)
                     /locus_tag="PK198_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41991.1"
                     /translation="LTSEVVVSASRVEERILESPVTIEKMDAIAIRQTAAPDFYDGIS
                     NLKGVQTTQGSMTLTSINTRGFGAVAN"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<225..547)
                     /locus_tag="PK198_24535"
     CDS             complement(<225..547)
                     /locus_tag="PK198_24535"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF41992.1"
                     /translation="MKTRFLPTLQWAILPIVLLLFAVAATAQTKISGMVSDKESGEPL
                     IGVNISVKGKLTGTITDFDGKYELNTSVAPPFTLVFSYTGFASQEVNVTGGQTTVDVK
                     MDAQS"
     gene            842..1222
                     /locus_tag="PK198_24540"
     CDS             842..1222
                     /locus_tag="PK198_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF41993.1"
                     /translation="MSLQDKYRAVLQLGEQLGVQEGFVEEKDGKLHIGGITQTQYEKN
                     LLWDKIKEIGGESFPDVAANIKVAVTDYYHKHTVSKGETLSKIAKQYYDDASKYMKIF
                     NANTDILKNPDLIQIGQELIIPNE"
     gene            complement(1307..>1862)
                     /locus_tag="PK198_24545"
     CDS             complement(1307..>1862)
                     /locus_tag="PK198_24545"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HRF41994.1"
                     /translation="AADCAFGYRDSFFKREGKGRYAIVKVYFQLTTSKHRLRLDYGAI
                     RDTLKEMNVAHPDIHDISRAIIQIRQSKLPDPALIGNAGSFFKNPETEADTWQRLSEL
                     HPGMPHFPLPDGRVKIPAGWLIEQCGWKGFREGDAGCYDKQALVLVNYGQATGQEILS
                     LARRIAASVQERFGMVIEPEVNVW"
     assembly_gap    1863..1874
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1875..>2202)
                     /locus_tag="PK198_24550"
     CDS             complement(<1875..>2202)
                     /locus_tag="PK198_24550"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF41995.1"
                     /translation="HILGGGSNVLLTGPVQGRVLHIRIPGRNVEAVSGNTALVAAGAG
                     ENWHDFVQWCLAQSLGGLENLSLIPGSVGASPIQNIGAYGVELKDVFHSLEAVRLSDG
                     ELVTMDA"
     assembly_gap    2203..2225
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2338..>2581)
                     /locus_tag="PK198_24555"
     CDS             complement(2338..>2581)
                     /locus_tag="PK198_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF41996.1"
                     /translation="ITDAIVEVHLEEAFYSTRFGLIKGAKATPSGYRAFFYYKDQLTK
                     VQVAEPLFPGKRRGDEAVLPLKTGFWGFEFVPKREP"
     assembly_gap    2582..2720
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2870..>3283)
                     /locus_tag="PK198_24560"
     CDS             complement(2870..>3283)
                     /locus_tag="PK198_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF41997.1"
                     /translation="EVIEAARQANAWDFISAFPDGLDTIVGERGVKLSGGQRQRVAIA
                     RAILKDPAILLLDEATSSLDAESEKAVQEALDKLMQGRTSIIIAHRLSTIREADCICV
                     IDGGRIVEQGTHAELSQLQDGIYNSLARLQFQTGL"
BASE COUNT          732 a          832 c          823 g          712 t
ORIGIN      
        1 cgttggccac agctccgaag ccgcgcgtgt tgatcgaggt cagggtcatg gagccttgcg
       61 tggtttgtac gcctttgagg ttggaaatgc cgtcatagaa atcgggcgcg gccgtttgcc
      121 ggatggcgat ggcgtccatt ttttcaatgg tgacgggcga ttccagaatg cgctcctcta
      181 cacgggaggc agaaaccacc acttcgctgg tgagnnnnnn nnnnatgctt tgcgcgtcca
      241 tctttacgtc cacggtggtt tggccgcctg ttacatttac ttcctgagaa gcaaagccgg
      301 tgtaggaaaa caccaatgtg aaaggcggag caacggatgt gttcagttcg tacttgccgt
      361 caaaatcggt gatggtgccg gtcaatttgc ctttgacgga aatattgacg ccgatgagcg
      421 gttcgccgga ttccttatcg gaaaccatgc cgctgatttt ggtctgagct gttgccgcca
      481 cggcgaacaa cagcagcaca atgggaagaa tggcccattg aagggtgggt aaaaacctgg
      541 ttttcatacg gttagattta atgattactg aaaaaagtct gttccgaaaa aaaattacgc
      601 taaattaaca ctactattga aacttggtgc gatcgggccg cttttcgcgc aaattatcct
      661 tatttccatg atggaaggtc gccggcaaat gtgacccaca ggtgcctctg cgtctcaatc
      721 gcggtttccg accggggcat gtagtttatg aagaaatcat tatctttacg cctgctccgc
      781 ttcgtgttac cgaaagggag taaatttgta tgttttttta actttttaaa accactcaat
      841 catgtcatta caagacaaat accgcgcagt gcttcaactc ggcgagcaac tcggcgtaca
      901 ggaaggcttc gttgaagaaa aagacggcaa actgcacatc ggcggtatca cccaaactca
      961 atacgaaaag aatctcctgt gggacaagat caaggagatc ggcggtgaaa gcttccccga
     1021 cgttgctgcc aacatcaaag tagctgtgac cgactactac cacaagcaca cggtatcaaa
     1081 aggcgaaacg ttgtccaaaa tcgccaagca gtactatgac gacgcgtcca agtacatgaa
     1141 gattttcaac gccaacacgg acattctgaa aaaccccgac ctgattcaaa tcggtcagga
     1201 gttgatcatt ccgaacgaat aagtaaaggg ggttccgacc ctcgtccgat aaatcataca
     1261 gagcaatctc cgcgaggggg ttactttttt ttgttcatct tcctcctcac cacacattca
     1321 cctccggctc tatcaccatc ccgaaacgct cctgcaccga tgccgcgatg cgacgcgcca
     1381 gcgacaggat ttcctgaccg gtagcctgtc cgtaattgac caacaccaat gcctgcttgt
     1441 cgtaacaacc ggcatcgccc tcccggaatc ccttccagcc gcactgctca atcagccagc
     1501 cggcgggaat tttgacacgc ccatcgggca gtgggaaatg aggcattccg gggtgcagtt
     1561 ctgaaagtct ttgccaagta tcggcctctg tttcagggtt tttgaaaaaa ctgccggcat
     1621 tgccgatgag ggccggatcg ggcaatttgc tttgccggat ttgaatgatg gcccggctga
     1681 tgtcgtgtat gtcggggtgg gcgacattca tttccttcag ggtatcgcga atggcgccgt
     1741 agtccaaacg gaggcgatgc ttggaggtcg tgagttggaa atacactttt acgatggcgt
     1801 atcggccttt gccctcgcgt ttgaaaaaac tgtcgcggta gccgaaagca caatcggcgg
     1861 cannnnnnnn nnnngcatcc atcgtcacca attccccatc cgacaaccgc acggcctcca
     1921 agctgtgaaa cacatccttc aattctacgc cataagcccc gatgttttga atgggagaag
     1981 ctcccaccga accgggaatg agcgagaggt tttccagtcc gcccaacgac tgcgccagac
     2041 accattgcac aaaatcgtgc cagttctcgc cggctccggc tgcaacgagg gcggtattgc
     2101 cggaaacggc ctccacgttg cggcccggaa ttcggatgtg caacacgcgg ccttgcaccg
     2161 gcccggtgag caatacgttg cttccgcctc ccaaaatgtg cannnnnnnn nnnnnnnnnn
     2221 nnnnntgccg ggcgatggcc tgtaaatctg aaacgctgtg gatttcgcaa taatgccggg
     2281 cttccgcttc gatgccgaaa gtgttgaacg ggcgcagagg gatgtgttgc tgatatgtca
     2341 tggctcgcgt tttggaacaa attcaaagcc ccagaagcct gtttttaaag gcagtacggc
     2401 ctcgtcgcct ctgcgcttgc cgggaaaaag cggttcagct acctgcactt tcgtcagttg
     2461 gtctttgtaa taaaaaaaag cccggtagcc gcttggtgta gctttggcgc ctttgatcag
     2521 gccgaagcgc gtgctgtaaa atgcttcctc caaatgcacc tccacgatgg cgtcggttat
     2581 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn cgcgcccgca atgagaccgg ccagcgctcc gtattgcacc
     2761 aattgactgc catgcaaggt gcggtgtatc cagccgattt cgagggcata caaaacgacg
     2821 actccgaaaa agagcagtgc tgaaaacgcg accaaccaag gagttttttt cataaaccgg
     2881 tttgaaattg aaggcgcgcc agactgttgt aaatgccgtc ctgcaattgg ctcagttcgg
     2941 cgtgcgtgcc ttgctctaca atgcggccgc cgtcaatgac gcaaatacag tcggcctcgc
     3001 ggatggtgga cagccggtgc gcaataatga tggaagtgcg gccctgcatg agtttgtcta
     3061 aggcctcctg tacggccttt tccgactcgg catccaagct ggaggtggcc tcgtccaaca
     3121 acaaaatcgc cgggtctttg aggatggcgc gcgcaatggc cacgcgttgc ctttggccgc
     3181 cggagagttt cacgccgcgc tcgcccacga tggtatccaa gccgtcgggg aacgcggaaa
     3241 tgaaatccca ggcattggcc tgccgcgcag cttcgatcac ttc
//
LOCUS       DAORLF010000492         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_15965_length_3279_cov_1.777916, whole genome shotgun sequence.
ACCESSION   DAORLF010000492 DAORLF010000000
VERSION     DAORLF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15965_length_3279_cov_1.777916"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>280)
                     /locus_tag="PK198_24565"
     CDS             complement(76..>280)
                     /locus_tag="PK198_24565"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF41998.1"
                     /translation="LTGKLKQLESILYGAAFFRCHHSSLVNLHFVKKIKTDDGVWLLL
                     MEGEAVPVSREKRKGLMERMYRL"
     assembly_gap    281..310
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(491..2491)
                     /locus_tag="PK198_24570"
     CDS             complement(491..2491)
                     /locus_tag="PK198_24570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF41999.1"
                     /translation="MNRFKEEHPWNIYSSDCWFDEVRDTLCLADLASLLALHNNNPHT
                     IFDADVCSQILCLSALPGAMNGFNKEQRKHVEDVQLMFDLQQQWLCEMPGINDEESLS
                     QLKYLAEKYTGHKVIFDLARQLGDYLVNYGKIASARKLAESLTPLFPDGKYCSVPQYF
                     HHQKQAWFESYAALLGRMTDDAPPAKPVAAWNTPDNDEYALVSWGSNDEVFFVRRNRA
                     TGAASVMTSKQKGGVWTVPEPVKALSVANDVVLLSMSENGQMMLLQSGGKLLQAFRRD
                     LQRPWTKPDPIPMMFRSRSKATLSPDGSMMVFAQYAPQKNEMLPPTTDIFVSYWDERN
                     QRYGTPKPVGKIINTEGNSEANPLIASGGRMIIFTSDRPGGLGGTDTYSAVLESPGDW
                     NTVATPQNMGLPLNTPLADKGLTFYSEYDGRGYFDRKNPCTNDINIWMLPGLRSDLFP
                     EALRLAGIVIDENGQPVKTGFMEFTTNYSLSVHSQALTTGGTYSFTTPDTSRVVRLFP
                     EVPGYYSERDTTYFLDNIPKGELLRDTFRVTSFEYIRRHFVLQHSTFFHGTAQFDNPA
                     KAYPELTRFAKIATRMGAELELTGHTDNVGTAESNDALSLQRAMSVKQFLVEKCGFDP
                     AKIKVSAKGGRAPIASNDTEEGRRLNRRIEVAFKMPELTGAK"
     gene            complement(2433..>3279)
                     /locus_tag="PK198_24575"
     CDS             complement(2433..>3279)
                     /locus_tag="PK198_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42000.1"
                     /translation="YSCTLQTKKKMKNLNLPPLRATRYAVLLLVAVLVSACPSFRSGY
                     RKMLAGEKKEARAIFKKNEKHKIFGPGATYYKVWLDAERTFIFSELGSLHSELCNLDK
                     RIAALPFPAQDKLAKYKVAPHEVLESVNKLERRILDQLCESGGVSHLDTLYSHFFCWR
                     NEEGRDSITTIIVNRSLHPAMPVYEDACEKWKQPVPDIKNISVPELKDNPGLPCVNRA
                     DRLRWSVNYQEATSIIRRYRNSVLDENIPEYWQLNHDVWSIFQRHESLQGGAPVEYLF
                     FRLLV"
BASE COUNT          705 a          830 c          848 g          866 t
ORIGIN      
        1 ctatggtctc cgtaagctcc ctcgttctct ttttcataca agcatcaaaa tagcccatat
       61 cccccccctt tcccctcaca accggtacat ccgctccatc agccccttgc gtttttccct
      121 cgatacgggt acagcctccc cctccatgag cagcagccat acgccgtcat cggttttgat
      181 tttttttaca aaatgtaaat tgaccaacga ggaatggtgg catcggaaaa atgccgcccc
      241 gtacaggatg ctttccaact gtttgagctt gcctgtcagc nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn gttcggatgc gtacataagc gccctcagcc ttggcatacg ccaaatcgct
      361 gaatttcaac agtatgtagc cggttttttc cggcatcagc agtatgggca gcttctccat
      421 gtctcttctt taccctattg gtgacagggg aagagaaaat ggtaccctcg ttgcgatttt
      481 tttttgaaaa ttattttgcc cccgtcaatt ccggcatctt aaatgccact tctatacggc
      541 ggttcagtct gcggccctcc tcggtatcgt tggaggctat gggcgcgcgg ccgcctttgg
      601 cagacacctt gattttggcc gggtcgaagc cgcatttttc taccaaaaac tgcttgaccg
      661 acatggcgcg ttgcagcgat aaggcgtcgt tcgattccgc tgtgcccacg ttgtcggtgt
      721 ggcccgtcag ttccaattcg gcgcccatgc gggtggcgat tttggcaaat cgtgtcagtt
      781 ccggataggc tttggcgggg ttgtcaaatt gggcggtgcc gtgaaaaaac gtgctgtgtt
      841 gcaatacaaa atgccggcga atgtactcga aagacgtaac ccggaaggtg tcgcggagca
      901 gctcgccttt gggaatattg tctaaaaaat aagtcgtatc ccgctccgaa tagtagccgg
      961 gtacttcggg gaacaacctg accacccgcg aagtatcggg cgtggtgaag gaataagtgc
     1021 cgccggtagt aagtgcttgc gagtgtacgc tgaggctgta gttggtggtg aattccataa
     1081 accctgtttt cacgggctgc ccgttttcgt ctatcacaat gcccgccaga cgcagcgctt
     1141 caggaaacaa atcggagcgg aggcccggca gcatccagat atttatatcg tttgtacagg
     1201 gattctttcg gtcaaaataa ccgcgcccgt cgtactccga atagaaggta agccctttat
     1261 ccgccagggg ggtattgagc ggtaagccca tattttgggg cgtcgctacg gtattccagt
     1321 ctccgggaga ttccagcacg gcgctgtatg tgtcggttcc acccagcccg cccggcctgt
     1381 cggaagtgaa aataatcatt cgcccgcctg atgcaatcag cggattggcc tcgctgtttc
     1441 cttctgtgtt gatgatctta cctactggtt ttggtgtgcc gtagcgttgg ttgcgctcgt
     1501 cccagtatga cacgaagata tcggtagtgg ggggcaacat ctcatttttt tgcggcgcat
     1561 attgggcaaa taccatcata cttccatcgg gcgaaagggt tgccttgctt cgggagcgaa
     1621 acatcatcgg tatcgggtcg ggttttgtcc aggggcgttg caaatctctt cggaaagcct
     1681 gcaacagttt tccaccggat tgtaagagca tcatttggcc gttttcgctc atagataaaa
     1741 ggactacatc gttggcaaca gagagtgctt tgacgggttc gggcacggtc cacacgccgc
     1801 ctttttgttt ggaagtcata accgatgcgg cgccggtagc ccggttgcgg cgtacaaaaa
     1861 acacctcgtc gttgctgccc caggaaacga gcgcatattc gtcattgtcc ggggtgttcc
     1921 atgcggcgac gggtttggca ggaggggcgt cgtcggtcat acgccccaac aatgcggcat
     1981 aagattcaaa ccaggcttgt ttttggtgat gaaaatactg aggcacgctg caatactttc
     2041 cgtcgggaaa cagcggggtc aggctttccg ccaatttccg ggcagaggcg atttttccat
     2101 agtttaccaa gtaatcgccc aattgtcgcg ccaggtcaaa gatgacttta tgcccggtgt
     2161 atttttcggc cagatatttc agttgagaca aactttcttc atcgttgatc ccaggcattt
     2221 cacagagcca ttgctgttgc aaatcaaaca tcagttgcac atcttccaca tgtttgcgtt
     2281 gctctttgtt gaagccgttc atggcgcctg gcaaggcaga caggcagaga atttgcgagc
     2341 atacatccgc atcgaatatc gtgtgagggt tgttgttgtg gagcgccaac aaagacgcta
     2401 aatccgccag gcaaagcgtg tcgcgcactt catcaaacca gcagtctgaa gaatagatat
     2461 tccacgggtg ctcctccttg aagcgattca tgtcgctgga atatgctcca tacatcatgg
     2521 ttcaattgcc agtattcagg gatgttttca tcaagtaccg agttgcggta acgccggatg
     2581 atgctggtgg cctcctgata attcacactc caacgcaggc gatctgcccg atttacgcat
     2641 ggcaagcccg gattgtcctt caattccggg acacttatat tttttatatc aggcacgggc
     2701 tgtttccatt tttcacaagc gtcttcatac acgggcatgg cgggatggag gctcctgttc
     2761 acaatgatgg tggtgatgga gtcgcggcct tcttcgttgc gccagcagaa aaagtggctg
     2821 tacagggtgt ccaaatggct cactccgccg ctttcgcaca gttgatcgag tatgcggcgc
     2881 tccagtttat tgacagattc aagcacttca tgtggggcca ccttgtactt agccagcttg
     2941 tcttgtgccg gaaacggcag cgctgcaatg cgtttatcca aattgcataa ctcactgtga
     3001 agactgccca gttcactgaa tataaaggtg cgctctgcgt ccaaccatac tttatagtag
     3061 gtggcgccag ggccgaatat tttgtgcttc tcgttctttt tgaaaatggc tcttgcttct
     3121 ttcttttcgc cggccaacat ttttcggtac ccgctccgaa acgaagggca ggcgctcact
     3181 aagactgcca cgagcaataa aacagcatac cgggttgcac ggaggggagg cagattcagg
     3241 tttttcattt ttttctttgt ttgaagggta caggaatag
//
LOCUS       DAORLF010000493         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16083_length_3260_cov_2.038066, whole genome shotgun sequence.
ACCESSION   DAORLF010000493 DAORLF010000000
VERSION     DAORLF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16083_length_3260_cov_2.038066"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>254)
                     /locus_tag="PK198_24580"
     CDS             complement(<1..>254)
                     /locus_tag="PK198_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF42001.1"
                     /translation="FFLAKSWYIVTFYFLTGYMIRDAEDVRRFFWAVFITLILTVVVV
                     LIRHSTYGFSFEDVYRVLSPFYRNHVSYAAIMAVFIPFVW"
     assembly_gap    255..289
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<290..694)
                     /locus_tag="PK198_24585"
     CDS             complement(<290..694)
                     /locus_tag="PK198_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF42002.1"
                     /translation="MSKNIPSSFSFIPSELLLFGVFACIVLASVFGAVYTEMYFLAGF
                     PAVLLLGYLAVVDFRKIYYLLLFSIPLSTEVVLPNGFGTDLPTEPLTVGLMLLFLLFW
                     FRHRSAVKADFLKHPVTLLLLLHWGWMVVPMLS"
     gene            790..>1149
                     /locus_tag="PK198_24590"
     CDS             790..>1149
                     /locus_tag="PK198_24590"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRF42003.1"
                     /translation="MKQILIIGGYGNAGRQIAQLLLQARSDTNIVIAGRNEERARQCA
                     DALRAVFPERGVQAARLDILDSAHLFRLVSEADFVVNASGTPLLMETFIQALLQTGKD
                     GLDTQLSTSAKHRVLEKY"
     assembly_gap    1150..1167
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1468..1917
                     /locus_tag="PK198_24595"
     CDS             1468..1917
                     /locus_tag="PK198_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42004.1"
                     /translation="MLLPEMEALPKLLPGLRETGFFVGGFNPITDYLILPVVMMMLKY
                     LPKGFWKYAASLFRWGINQTKPPYGIMLAADCVGYEGNTRREGTITLFHEDAYIMTAA
                     PVVACLEQYFEAENKRPGVWLQADFVEPLEYITRLKSLGIRVEDKLI"
     gene            complement(1920..2774)
                     /locus_tag="PK198_24600"
     CDS             complement(1920..2774)
                     /locus_tag="PK198_24600"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRF42005.1"
                     /translation="MKRVLLLTVIAFAAILHAHAQLPDADSSLIAFLRKGPHISAHRG
                     GMNFAAYPENCLETFEHLLSQIPAIIELDVEMTADSVLIILHDNTLHRTTTGAGAVAA
                     HTWEQVKDLKLKNRDSTDTGFSIPLLADALEWAARRGAIFTIDVKRNVPFEKVIQQVE
                     RYKMERQSVIITYTAADALQVYTLKPDILISANMRSVEEADRVLGTGIPADRLVAFVG
                     TREPSPELYDKLKALNIPTILGTMGNLDNMALAKGNRVYLEFIARGAVILSTDRPVEA
                     WQAIMEGE"
     assembly_gap    2992..3006
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3021..>3260
                     /locus_tag="PK198_24605"
     CDS             3021..>3260
                     /locus_tag="PK198_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF42006.1"
                     /translation="MLVGFSTQAHAQVTTSSMGGKITDDKGEALIGATVLATHVPSGT
                     RYGTTTGVDGTYYISSMRSGGPYKVEVSYTGYAASV"
BASE COUNT          856 a          753 c          829 g          754 t
ORIGIN      
        1 accacacaaa tggaataaac accgccatga tggccgcata cgacacatga ttgcgataaa
       61 acggagagag cacccgataa acatcctcaa aagaaaaacc ataagtggag tgccgtatca
      121 ataccactac taccgtcagt atcagcgtaa tgaagaccgc ccaaaagaaa cggcgcacgt
      181 cttctgcatc ccgtatcata tagccggtga gaaagtaaaa ggtgacgatg taccagcttt
      241 tggcaaggaa aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ctgagcatgg
      301 gaaccaccat ccaaccccaa tgcaacagca acaacaatgt gaccggatgc ttcagaaaat
      361 cggccttcac cgcgctccga tgccgaaacc agaacaacag aaacagcaac atcagcccca
      421 cggtgagcgg ctcggtgggc aggtcggtgc caaaaccgtt ggggagcact acctccgttg
      481 agagcggaat agaaaaaagc agcagataat agatttttct aaaatcaacc accgccagat
      541 atcccagcaa cagcaccgcc ggaaagcccg ccagaaagta catttcggta taaacagccc
      601 cgaaaaccga tgccagtacg atacatgcaa aaactccgaa cagcagtagc tctgacggaa
      661 tgaaggaaaa tgaagatggg atgtttttgc tcatctggcc gcaaatttag aaatgtttct
      721 ctttgataat atttgtcatt gcttatcgga caaggtttct tcttatttgc agtattaaac
      781 ataggggcca tgaaacaaat actcatcatc ggcggatacg gaaatgccgg acgtcaaatc
      841 gctcaattac tgttgcaggc tcgtagcgat acaaacattg tcattgcagg cagaaacgag
      901 gagcgcgcac ggcaatgcgc agacgctctt cgggcggtat ttcccgaaag aggagttcag
      961 gctgcccgtc tggatattct ggacagcgcc catttgttcc gattggtttc ggaggcagat
     1021 tttgtcgtca atgcttccgg cacgccgctg ctgatggaaa cattcattca ggcattgctc
     1081 caaaccggca aagatggatt ggacactcaa ctctcaacct ctgcaaaaca cagggttcta
     1141 gaaaagtatn nnnnnnnnnn nnnnnnntta aaaacagcgg gtatttgtta catcacagac
     1201 ggcggctttc accccggagt gccgggcgct ttggtgcggt atgcggcttt gaaaatggag
     1261 cgtcttgaaa aagccaatat ttacagtgcc atgaaggtaa attggaggag cttcgagttg
     1321 tcaaaagaaa cttgggagga gtttatgggc gaattttcag actaccgaac cgaatgctgc
     1381 cgcgacggaa aatggcagaa gaccggttca atggagtatt tttcctacaa tttcggcccg
     1441 ccgttcggca aacaaaattg tatgccgatg ctcctgcctg aaatggaagc tttgccgaag
     1501 ttgctgccgg gtttgcggga aaccggattt tttgtcggcg gcttcaatcc gatcaccgat
     1561 tatcttattt tacctgttgt gatgatgatg ttgaagtatc tgccgaaagg cttctggaaa
     1621 tatgccgcca gcttgtttcg gtggggaatc aaccagacga agccgccgta tggcatcatg
     1681 ttggctgctg attgtgttgg ttatgaagga aatacaaggc gagagggaac catcacactt
     1741 tttcatgaag acgcatatat aatgaccgcc gcgccggtag tcgcttgtct ggaacaatat
     1801 tttgaggcag aaaacaagcg ccccggtgtg tggttgcaag ccgattttgt ggagccgttg
     1861 gagtatatca ctcgcttgaa atcattgggc atcagagtgg aagataaatt gatttgacat
     1921 tactccccct ccataatcgc ctgccaggcc tccaccggtc ggtcggtgga caatatcaca
     1981 gcgccacgag caatgaactc cagataaacc cgattgcctt ttgccagggc catgttgtct
     2041 aagttgccca tagtacccaa tatggtggga atattcagtg ctttgagctt gtcgtacagt
     2101 tcggggcttg gctcgcgggt acccacaaaa gcaacgagac ggtcggcagg tatgccggta
     2161 cccaacacgc ggtcggcttc ttctacactg cgcatattgg cggagatgag gatgtcgggt
     2221 ttgagggtat atacttgcag cgcatctgcc gctgtgtaag taatgatgac ggattggcgc
     2281 tccattttgt acctttccac ctgttgaatg actttttcaa aaggcacatt ccgcttcacg
     2341 tctatggtga aaatggcgcc gcgccgggca gcccattcca gcgcatctgc cagcagaggg
     2401 atcgagaatc cggtatcggt actatctctg tttttgagct tcaagtcttt cacttgttcc
     2461 caggtatggg ccgccactgc gcctgctccg gtagtggtgc ggtgcagcgt attgtcatgc
     2521 aggatgatga gtacgctgtc ggcagtcatt tccacgtcca attcaatgat ggcgggtatt
     2581 tgggaaagca gatgctcaaa agtttcgagg cagttttcgg ggtaagccgc gaaattcatg
     2641 ccgccccggt gcgcgctgat atgcggcccc ttgcgcaaaa aggcaatcag gctggagtcg
     2701 gcatcgggca actgagcatg ggcgtggaga atagctgcaa aagcaatgac agtaaggagg
     2761 aggacgcgtt tcattttata ccaattggtg actgattcaa cgaaaataca ggggggaggc
     2821 ggtgtgtaaa cgagctggat ttccaaaggc tggagtgaaa acgtgaaaaa ttcatattcg
     2881 gtttacaatc tctgcttacc tttgcacttg ttaagaaacg ttaacacttt ctctctttaa
     2941 caaaaatcca ttttatgaga agcattctac agaacgcagt cagaggggta cnnnnnnnnn
     3001 nnnnnncttt gacggttgcc atgcttgtgg gcttttccac gcaggctcat gctcaggtta
     3061 ccacctcttc gatggggggt aaaattaccg acgacaaagg cgaagcgctc atcggcgcta
     3121 cggttttggc aacacacgtt ccatccggta cgcggtatgg cacaaccaca ggggttgacg
     3181 gcacatacta catttccagt atgagaagtg gcgggccata caaagtcgaa gtgtcctaca
     3241 cgggatatgc agcttctgtt
//
LOCUS       DAORLF010000494         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16174_length_3246_cov_2.289878, whole genome shotgun sequence.
ACCESSION   DAORLF010000494 DAORLF010000000
VERSION     DAORLF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16174_length_3246_cov_2.289878"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PK198_24610"
     CDS             complement(<1..1145)
                     /locus_tag="PK198_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42007.1"
                     /translation="MKHSNKLIELLKTFSASELLAFRDFTASPYFNKREELVRLYAHL
                     KKAAASDFPDKKIERHLLFEAAFPGKPYDEALLHHSLSRLLKLAEQFIALEDVLLPRL
                     NSTLAPAQAYYRRKLRRHFDHAMKQAKTALEEQNLRDSEYFFVRHEMAALEEMYFSDL
                     NEQQLSGDSIQQASDFLDRSYLAKKLRFLCVMLDRQHFLASPARMDMKDEVLAFLAHH
                     DFSDTPAISVYHALLLALTHSEERACFEQFKALWTNSIEHFSMYDLKSQLGLAINFCI
                     HRIRLGDKSYAAMLADLYREGLERKILLDEGRISPWTFKNIVKLNLGLKRYEWAESFV
                     REYSHSLQEDERQDALHFNLAELYYHQGHLDEARAHLVKVEFSHWRY"
     gene            complement(1142..1834)
                     /locus_tag="PK198_24615"
     CDS             complement(1142..1834)
                     /locus_tag="PK198_24615"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRF42008.1"
                     /translation="MQIPERPNIERISFRSILDDLLGILNLERGIPYTIRRLITDPQG
                     AVREYLFEDRRRMAKPLPLLVLLVALAAYVSFRFLPIEGDVLEQVRLSPNSASIPPLI
                     MRTLELYLRAIQQYFNLTYMSTIPFQALATWLMYRHLRLHYMEHLVINVYIFCIQTII
                     YVLAMPLSIKFAPIAFLQAILSIGYFLYALHRIFGMGWIETIARAVGIWVMLQVAQVF
                     ILLGFLIAALMP"
     gene            complement(1900..2049)
                     /locus_tag="PK198_24620"
     CDS             complement(1900..2049)
                     /locus_tag="PK198_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42009.1"
                     /translation="MKIIKISVKKNFALGFKAAPRRQARLPVGKRGSGLQINCAADSP
                     EGDKL"
     gene            2266..>3246
                     /locus_tag="PK198_24625"
     CDS             2266..>3246
                     /locus_tag="PK198_24625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRF42010.1"
                     /translation="MHPDFRIQMQAQLGAAFADFADALTQPAPVSIRANRHKWTGPAQ
                     HDGQVPWCKNGYYLAERPPFTLDPLLHAGAYYVQEASSMSIAHALRQLVDLENPLLAL
                     DLAAAPGGKSTLLAGMLSADSLLIANEVIRNRYQALRHNLARWGLPNVAVSQHDPEDF
                     APLAGSFDLVLVDAPCSGEGLFRKDPDAQKEWSPAQVGFCAARQRRILAQAAALMAPG
                     GVLVYSTCTYNDEENAANAAWLQEVAGLEYAPVNWPSEWGVEERRWGYQFYPHRVRGE
                     GFYVACFRRVGGGRLAVGGGRAVARGGRWAVGSGREVLERVRKACGLGASR"
BASE COUNT          716 a          776 c         1042 g          712 t
ORIGIN      
        1 atatagcgcc agtgcgagaa ttccactttt accaaatgtg cgcgcgcctc gtccaagtgg
       61 ccctgatggt agtacaattc ggccagattg aaatgcaggg cgtcctggcg ttcgtcttcc
      121 tgaaggctgt ggctgtattc ccgtacaaaa ctttcggccc actcgtatcg cttgaggccg
      181 aggttgagtt tgacgatgtt tttgaaagtc cagggcgaaa tgcggccttc gtccaagagg
      241 attttgcgct ccaatccctc gcggtacaga tcggccagca tggccgcata gcttttgtcg
      301 ccgaggcgaa tgcggtggat gcaaaaattg atggccaggc ccaactggga tttgagatca
      361 tacatcgaaa aatgctctat ggaattggtc cacagcgctt tgaactgctc aaaacacgcg
      421 cgttcttcac tatgggtaag ggccagcagc agcgcatgat acacggaaat ggcaggcgta
      481 tcggagaagt cgtgatgagc cagaaaggcc agcacctcgt ccttcatgtc catgcgggcc
      541 ggcgaagcga ggaagtgctg ccggtcgagc atgacgcaga ggaagcgcag ttttttggcg
      601 aggtagctgc ggtctaaaaa gtcggaagcc tgctgaatgg aatcgcccga tagttgctgt
      661 tcgttgaggt cggaaaagta catttcttct aaggcggcca tttcgtggcg gacgaagaaa
      721 tactcgctgt cgcggagatt ttgctcttcc aaagcggtct tggcctgctt catggcatgg
      781 tcgaaatgcc ggcggagttt gcgtcggtaa taggcttggg ccggagcgag tgtggaattc
      841 agacggggca gcagcacgtc ttcgagcgct ataaattgct cggccaattt gagcagtcgg
      901 ctgaggctgt gatgcagcag ggcctcgtca tagggttttc cgggaaaggc cgcttcaaag
      961 aggaggtggc gctcaatttt tttatcggga aaatcggatg cggcggcttt tttgagatgt
     1021 gcgtacaggc gcaccaattc ttcccgcttg ttgaaatagg gcgatgcagt gaagtcgcgg
     1081 aaagccagca actcgcttgc cgagaaggtc ttgagcagtt cgatgagttt gttgctgtgt
     1141 ttcatggcat caacgcggcg atgagaaatc ccaacaaaat gaaaacctga gccacttgca
     1201 acatcaccca gatgccgacg gcgcgagcga tggtttctat ccagcccatg ccgaaaatgc
     1261 ggtgcagggc gtacagaaaa tagccgatac tgaggattgc ctgaagaaag gcaatgggag
     1321 caaattttat ggacaaaggc atggccaaaa catagataat ggtctgaata caaaagatgt
     1381 acacgttaat gaccaaatgc tccatatagt gcaggcgcag gtgacgatac atgagccagg
     1441 tagcgagcgc ctgaaaagga atggtactca tgtaggtgag gttgaagtat tgctgaatgg
     1501 cccggaggta cagttccagc gtgcgcataa tgagcggcgg aatggatgct gaattcggcg
     1561 aaagcctgac ttgttccaat acgtctcctt cgatgggcag aaagcgaaag gagacgtaag
     1621 ccgccaatgc taccaacagc accagcaagg gcaggggctt ggccatgcga cggcggtctt
     1681 caaaaagata ctcgcggaca gcgccctgcg ggtcggtgat gagccggcgg atggtgtagg
     1741 ggatgcctct ttcgaggttg aggatgccga gcaaatcatc cagaatgctc cgaaatgaga
     1801 tgcgttcgat gtttgggcgt tcgggtattt gcatgtgccg gagaggttga tggttgtgtt
     1861 ggagagattg cgccgggcga agttagggag gaatctgctt cacagtttat ctccttcggg
     1921 ggaatctgct gcgcagttta tctgcagccc ggaaccacgc ttgcctaccg gcaggcgggc
     1981 ctgtcgtcgt ggcgcggcct tgaaccccaa cgcgaaattt tttttgacgc tgatctttat
     2041 gattttcatg attttcgctg ctcggagagg ggcataacaa tcataagaat ctgcgccttt
     2101 cagcgtcaga aatccgccca ttaacccgga aatccgaaat caactcgttc ttacctgtca
     2161 atcagaagtc cgaaatcagt ccgctcccgc ccgaaattcc gaaatcaacc cattcctgct
     2221 gaaaatacta actttgcgat gaaacaacag acattctgaa cattgatgca tcccgatttt
     2281 agaatacaaa tgcaggcgca attgggcgct gcgttcgccg attttgccga tgcgctgacg
     2341 cagcccgcgc cggtgagcat acgggccaac cggcacaaat ggaccggacc tgcgcaacac
     2401 gacggccagg tgccctggtg taaaaacggc tactatctgg ccgagcggcc tccgtttacg
     2461 ctcgatccgc tgctgcacgc cggcgcgtat tatgtgcagg aggcttcttc gatgagcata
     2521 gcccatgctt tgcggcagtt ggttgatttg gaaaatccct tgctggcatt ggatttggcg
     2581 gcggcgccgg gaggcaaatc cacgctgttg gcgggcatgc tcagtgccga tagtttgttg
     2641 attgccaatg aagtgattcg caaccggtat caggcgctgc ggcacaacct ggcccgctgg
     2701 ggcctgccca atgtggccgt gagtcagcac gaccctgagg actttgcgcc attggccggc
     2761 agcttcgatc tggtgctggt ggatgcgccg tgttcgggcg aagggctgtt caggaaagac
     2821 cccgacgcgc aaaaagagtg gtcgccggcg caggtgggat tttgcgcggc gcggcagcgg
     2881 cgcattttgg ctcaggcggc agcgctgatg gcgcccggcg gggtgctggt gtatagtacc
     2941 tgcacctaca acgacgagga gaatgccgcc aacgcagcgt ggttgcaaga ggtggcgggc
     3001 ttggaatatg cgcccgtgaa ttggccgtcg gaatgggggg tggaggagcg ccgttggggg
     3061 taccaattct atccgcatcg ggtgcgcggg gaggggtttt atgtggcgtg ttttcggagg
     3121 gtaggcggtg ggcggttggc agttggcggt ggccgcgctg tggcgcgggg tgggcggtgg
     3181 gcagtaggca gtgggcggga ggtgctggag cgggtacgga aggcgtgcgg gctgggagcg
     3241 agccgg
//
LOCUS       DAORLF010000495         3236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16252_length_3236_cov_1.749135, whole genome shotgun sequence.
ACCESSION   DAORLF010000495 DAORLF010000000
VERSION     DAORLF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16252_length_3236_cov_1.749135"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PK198_24630"
     CDS             complement(<1..775)
                     /locus_tag="PK198_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42011.1"
                     /translation="MPTTFLNFKNWLKWAVSLALLAAPTLSQSCIWYDLDEGRVWIFA
                     PNLVNIPSLLPFTYTYERYYAPYDYPEQEPAHYRQNVREWQAAAGAQAKEVDVYAVLY
                     TITPQDYFSGKGLKTNSFIVQLAKPENRELKQYLDFAKRCEALMNDQDRAWSQNPVQG
                     FAELTTEGEALLAAAQSPFVQLRAAYQVMKLHQYQEQYDRGLAVYKQYIEPHKGNTWI
                     TGSALYHYALMQSDPVETNYWYARAFDAFEGNRFVIQLSY"
     gene            complement(726..>1681)
                     /locus_tag="PK198_24635"
     CDS             complement(726..>1681)
                     /locus_tag="PK198_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42012.1"
                     /translation="PHSPVPAFYHWKNKLSLSAEDASTLQDFAAKTLYIRYFDVQWSG
                     GGALPVSVTKLDWASLPEATAVIPVVFITNETFRNLSLEQSAQLAAKTAQKIQAMSEG
                     VVFEEVQIDCDWSACTRERYFLFLNELRKNLPSVQLSVTIRLHQVKYRTEAGIPPADR
                     GMMMPYNLENPARFSLQNSIYDSREAAKYLRQQPPYPLPCDVALPLFSWGVQFRGEEF
                     RGFLHGLRPEQAQGLSFLQKEDGIFYRVTADTLFQNLYLRRGERIKTESVSTDNMAEM
                     LRLLAPVLPNDSFRMTFFHLDQPVIHAYDFSQLQKLAQVGR"
     assembly_gap    1682..1732
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1798..>2639)
                     /locus_tag="PK198_24640"
     CDS             complement(1798..>2639)
                     /locus_tag="PK198_24640"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF42013.1"
                     /translation="VDERLSEDFSDVVMLCDTRSGETVSIPFILEHKSYLPAHPPFQV
                     MHYQDRVWQQQIRDSTLALTPVVPIMFYHGQEEWIVKPWSAYLKGWQEVFQPFTPTGG
                     YIFIDLSGMPDEEITTFRSGFLKTALLLMKHRLEREFLLKNLRIIINFVESDSTPSEV
                     TVENLKIVLRYLQGLRVMKWEEVRKQLQPLTLTNSVMNIWDEIKMEAWEEGKEEGINI
                     GIEKGIEKGIEKGIEKGIEKEAFAIISSLIRHLPEANDETIANLTSKPVEWVARVRLE
                     MNA"
     assembly_gap    2640..2662
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2789..>3236)
                     /locus_tag="PK198_24645"
     CDS             complement(2789..>3236)
                     /locus_tag="PK198_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRF42014.1"
                     /translation="QAKGVQMGSIGGGGRYDNLTGIFGLEGVSGVGVSFGADRIYDVM
                     EELNLFPAEALDSLRLLFIAFDEATHRYAFQLLGQVRAAGINAELYPEPAKMKKQMKY
                     ANDRRVPFVAIIGSEEMAAGEVTLKNMETGEQVRVGAENLIEQMKK"
BASE COUNT          743 a          818 c          814 g          787 t
ORIGIN      
        1 tgtaagacaa ctgaatgaca aagcgattac cctcaaaggc atcgaaagcg cgggcgtacc
       61 agtagtttgt ttctacaggg tcggactgca tgagagcgta gtggtacagg gcagaaccgg
      121 tgatccaggt gttgcctttg tgcggttcga tgtactgctt atacactgcc aaacctcggt
      181 cgtattgctc ctgatactga tgtagtttca tcacctgata agcggcgcgc aattgtacaa
      241 agggcgactg cgcggcggcc agcagggcct ctccctcggt tgtcaattct gcaaaacctt
      301 gtacaggatt ttggctccag gcgcgatctt ggtcgttcat caatgcctcg cagcgtttgg
      361 caaagtccaa atattgcttc aattcccggt tttccggctt ggccaactgg actatgaatg
      421 aattggtttt caatccctta ccgctgaaat aatcttgtgg ggtaatagtg tacaacaccg
      481 catatacatc tacttctttc gcctgagcgc ccgccgcagc ctgccactcg cgcacatttt
      541 ggcgataatg cgccggttct tgttcggggt aatcataagg agcataatac cgctcgtagg
      601 tgtaggtgaa aggcagcaga ctgggaatat tgaccaaatt gggggcaaat atccatacgc
      661 ggccttcatc caaatcgtac cagatacaag attgagaaag cgtgggtgct gccaaaagcg
      721 cgaggctaac ggcccacttg agccagtttt tgaagttgag aaaagtcgta ggcatggata
      781 acaggttgat cgaggtgaaa aaaggtcatt cggaagctgt cgttgggcaa tacgggagcg
      841 agcagccgga gcatttcagc catgttatcg gttgaaacag actccgtttt gatgcgttct
      901 ccccggcgaa gatagagatt ctgaaacagg gtatcggcag tgacgcggta aaaaattcca
      961 tcctcctttt gcaggaatga taaaccttgt gcctgctccg gccgtaagcc atgcaaaaag
     1021 ccccgaaact cttcgccgcg aaactgtacg ccccatgaaa acaaaggcag ggccacatcg
     1081 cagggcagcg ggtagggcgg ctgttggcgg agatatttag ctgcttcccg gctgtcgtaa
     1141 atggagtttt gcaggctgaa tcgggcggga ttttccaaat tgtagggcat catcataccc
     1201 cggtcggcag gtggaatacc ggcttctgtg cggtacttca cctggtgcag gcggatggtg
     1261 acgctgagtt gaacggaggg cagatttttt ctcaactcgt tcagaaacag gaaataacgc
     1321 tcgcgggtgc aggcgctcca atcgcagtct atctgcactt cttcaaaaac tacgccctcg
     1381 ctcatggcct gtattttttg cgctgttttg gcagccaatt gagccgactg ctccaagctg
     1441 aggttgcgaa aggtttcgtt ggtgatgaat acgacaggaa tgacggcggt tgcttcagga
     1501 agacttgccc aatctaactt cgtcacagaa acaggcagag cgccgccgcc cgaccattgc
     1561 acatcaaagt acctgatgta gagggttttg gcagcgaaat cctgcaaggt ggaagcatct
     1621 tcggcagaca ggctgagttt gtttttccag tggtaaaaag cgggaacggg tgagtgcggg
     1681 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggagggta
     1741 ttggtgcgca aggcagaaaa attttataga ccgatgcagt ctccaaaaca atgccgctca
     1801 agcgttcatt tcgagccgca cccgcgccac ccactctacc ggcttggagg tgaggttggc
     1861 tatggtctcg tcattcgcct ccggcagatg cctgatcaga gaggaaatga tggcaaatgc
     1921 ttctttctcg atacccttct cgataccttt ctcgataccc ttctcgatac ccttctcgat
     1981 acctatattg attccctctt ctttgccttc ttcccaggct tccattttga tttcgtccca
     2041 gatattcatg acggaattgg ttaaagttaa aggttgcaat tgttttctga cttcttccca
     2101 cttcataacc cgaagacctt gcaaataacg cagcactatc tttaggttct caacggtcac
     2161 ttccgagggt gtggaatctg actctacaaa gttaatgatg attcgcaagt ttttcaacaa
     2221 aaattctctt tccaaccggt gcttcatcag caatagggct gttttgagaa agcccgaccg
     2281 aaatgtcgtg atctcctcat cgggcattcc cgaaaggtca ataaagatat aaccgcctgt
     2341 tggcgtgaat ggttggaata cctcctgcca gcctttgaga tatgccgacc agggttttac
     2401 aatccactcc tcctggccat gataaaacat aatgggaacc acgggtgtga gcgccagtgt
     2461 ggagtctcga atctgctgtt gccacactct gtcctgatag tgcatgacct gaaacggcgg
     2521 atgagccggc aaataggact tgtgctccag aatgaatggg attgaaacgg tttcaccaga
     2581 ccgggtgtcg cagagcatca ctacgtctga aaagtcctca gaaaggcgtt cgtccacatn
     2641 nnnnnnnnnn nnnnnnnnnn nnaatccaaa atggcgagca gttctgccgg caatttcgcg
     2701 gccagcaact cccgaacgtt gtcggcattt ttgaacacag ccttgaacag gcggtctgcg
     2761 ggatgatggg gtttgttgga catatctttt actttttcat ctgttcaatc aaattttctg
     2821 cccccactct cacctgctcg cccgtctcca tatttttgag cgtcacctcg cctgcggcca
     2881 tttcctcgct gccgatgatg gccacaaaag gcacccggcg gtcgttggca tacttcattt
     2941 gctttttcat cttggccggt tcggggtaca actccgcatt gatgccggcg gcccgcacct
     3001 gccccaagag ttggaaggca tagcggtgcg tagcctcgtc gaaggcaatg aacaacaggc
     3061 gcaaactgtc cagcgcttca gccggaaaca gattcaactc ttccatcaca tcgtagatac
     3121 ggtcggcccc gaacgatacg cccacccccg acacgccctc taatccgaaa atgccggtga
     3181 ggttgtcgta cctgccgccg ccgccgatgc tgcccatctg cacgcctttg gcctgc
//
LOCUS       DAORLF010000496         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16259_length_3235_cov_1.966038, whole genome shotgun sequence.
ACCESSION   DAORLF010000496 DAORLF010000000
VERSION     DAORLF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16259_length_3235_cov_1.966038"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>274)
                     /locus_tag="PK198_24650"
     CDS             complement(78..>274)
                     /locus_tag="PK198_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42015.1"
                     /translation="IHATMMETGKPIVQTTILLFFGFLVLLFSISPPSVAIGIIISAT
                     LFSALVIDLLLLPLLLRKWG"
     assembly_gap    275..380
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..2322)
                     /locus_tag="PK198_24655"
     CDS             complement(<381..2322)
                     /locus_tag="PK198_24655"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRF42016.1"
                     /translation="MERIATFRKVMVVAAFALLGGLSVYYGTKVRFSFDFEQFFPEGD
                     EELAFFRTFVKDFESDENFMLIAVRREDGVFEQEFLEKVHDLTLKAGHLPYVVESQSL
                     TKWGYPIITPFIITTIPAIHRDDPDHYAEDKARILADERFVYNFITPDARTLTVALKM
                     MDYIPLKESREFMRALDKLMKQYEFEEYHYLGRPYFQRELVAMQQREILVSALISGLL
                     VALLLWLLLRRFWGVFISLISIALSMLLFVGFLGVTGRELSMLSALYPLLMIIVSTSD
                     VVHILSKYQDELLKGYDRKTAITTTIKEIGLATFMTSATTAVGFLSLFTSRIVPIREF
                     GVNAAVGVMLAFVTVIVFTTTLLTWFDANQISRLGKGQENWNKLLNRIYLFTVQKQRS
                     IVWGTLVVIAVSIAGISMVTTNYDIVNNLPRGKKITEDFLFFEREMAGFRPVEFAVFV
                     QKGYQADDYAVLRQLDTVEQYLRSFPDIHAVNSITTVYKSIHRMKANNDPAAYRLPPD
                     EEQHESYRRLASKIPKSGADILISKDGTKARITSRVKDCGADSIKRIYNQIDQYIASH
                     TDPNVVKFRRTGTGIIIDKNAAYVRSSLFQGLLMSILIIGGLMGLIFKNLRMLLISMV
                     PNVIPLLIAGALMGFFDIELEAG"
     gene            2376..3158
                     /locus_tag="PK198_24660"
     CDS             2376..3158
                     /locus_tag="PK198_24660"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HRF42017.1"
                     /translation="MNTLPTALEKYSKKIGATQRPFVRIHLEQADNLLPWNSKVGGAP
                     YLPKGIPFPVNAEGVQLFFMAQVNFAEMPPLEGFPLKGILQFFIFDDGLYGLDFDEPE
                     AQHNFRVLYYADPVTDSDALITDFTFLRSYGDDMPLKAGSEWAMRFQPDTGLMPESDF
                     RFDGIFGEDFFARFGTDEWSIAEAYGKISDAAGHRVGGYAEFTQEDPRQPEDLMELLF
                     QLDTDSKHGILWGDMGVANFFIRPEDLAQLNFSRVLYNWDCY"
BASE COUNT          778 a          839 c          794 g          718 t
ORIGIN      
        1 gggatcaccc tcaacgggcc atcactcgat tcgctccgct tcggcattca tacccggcag
       61 cttaattttc agtaaaatca gccccacttg cgcaacaaca gcggcagcag cagcaggtca
      121 ataactaatg cgctgaataa agtggcgctg atgatgatgc cgatggccac gctgggcggg
      181 ctgatggaaa acaacaatac caaaaagccg aagaacagca gtatggtggt ttgaacaatc
      241 ggcttaccgg tttccatcat agtggcatgg atggnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ccccggcctc caactcaatg tcgaaaaagc ccatcagcgc
      421 tccggctatc agcaacggaa tcacattggg caccatcgaa atgagcaaca tgcgaaggtt
      481 tttgaaaatc agccccatca agccgccgat gataaggata gacatcagca gcccctgaaa
      541 aaggctgctg cgcacatacg ctgcattttt atcaatgatg atgcccgtac cggtacgccg
      601 gaatttgacc acattggggt cggtatgcga agcgatgtat tggtctattt ggttgtaaat
      661 ccgcttgatg ctgtcggcgc cgcagtcctt cacccgtgag gtgatgcgcg cttttgtgcc
      721 gtctttgctg atgagaatat cggcgcccga tttggggatt ttggaagcta agcgccggta
      781 cgattcgtgt tgctcctcgt cgggcggcaa gcggtatgca gcgggatcgt tgttggcctt
      841 catccggtga atggatttat acaccgtagt aatggagttg acggcgtgaa tgtcggggaa
      901 gctgcgcagg tattgttcta ccgtatccaa ttgcctgagt acggcgtagt cgtcggcctg
      961 gtaccctttt tgtacaaaaa ctgcaaattc gaccggcctg aaaccggcca tttcccgctc
     1021 aaagaacagg aagtcctcgg tgattttttt gccgcgcggc aggttgttca caatgtcgta
     1081 gttcgtcgtt accatcgaaa taccggctat tgaaacagca atgacgacaa gcgttcccca
     1141 aacgatactg cgttgctttt gtacagtaaa aagatagatg cggttgagca gtttgttcca
     1201 gttttcctgg cctttgccta ggcggctgat ctgattggca tcaaaccaag tgagcagcgt
     1261 tgtggtaaaa acaatgactg taacaaaggc cagcataact cccactgcgg cattcacccc
     1321 aaactcgcga atgggcacaa tgcgactggt aaacaacgac aaaaagccta ccgccgtagt
     1381 ggcagaggtc ataaaggtag ccaacccgat ttctttgatg gtagtggtga tggccgtttt
     1441 tcggtcgtaa cctttgagca actcatcctg atatttagac aaaatatgca ccacgtcgga
     1501 ggtactcaca ataatcatca gcagcggata cagggccgac aacatgctca gttcgcggcc
     1561 ggtaacgccc agaaacccca cgaacaacaa catgctcaac gctatggaaa tgagcgatat
     1621 aaacacgccc caaaagcgcc gcagcaacaa ccacaacaac agcgccacca acaagcccga
     1681 aatgagcgcc gacaccaaaa tttcgcgctg ctgcatggcc accaattcgc gctgaaaata
     1741 aggccgcccc aagtagtgat actcctcaaa ttcatactgc ttcatcagtt tgtctaatgc
     1801 gcgcatgaat tcccgcgact ccttcagcgg gatgtagtcc atcatcttga gggccaccgt
     1861 gagggtgcgc gcatcggggg tgatgaagtt gtaaacgaag cgctcgtcgg ccagtatgcg
     1921 ggccttgtct tcggcgtagt gatcggggtc gtctcggtga atggcgggta tggtggtaat
     1981 gataaatgga gttatgatcg gatagcccca tttggtcaac gactgactct ctacaacgta
     2041 cggcaggtgg ccggctttga gggtcagatc atgtactttt tctaaaaact cctgctcgaa
     2101 caccccgtcc tctcggcgca cggcaatgag catgaagttt tcatcggatt caaaatcctt
     2161 gacaaacgtt cggaaaaacg ccagttcttc atctccttcc gggaaaaact gctcgaagtc
     2221 gaaggaaaac ctgaccttcg tgccgtaata aacagaaagc ccgcccaaca gagcaaaggc
     2281 ggccaccacc atgaccttgc gaaaagttgc tattcgttcc atactcgccg gcttaaccgc
     2341 tccggctgcg aaatgtttat aaaaaaagac ttgttatgaa cacgcttcct acggcactcg
     2401 aaaaatacag caaaaaaata ggagctacac agcgcccgtt tgttcgtatt catctggaac
     2461 aggccgacaa cctactgccc tggaacagca aagtaggcgg cgcgccttac ctgcccaaag
     2521 gtattccttt cccggtgaat gcagagggtg tacagttgtt ttttatggcg caggtcaatt
     2581 ttgccgaaat gccgcccctc gaaggtttcc ccctcaaagg cattcttcag ttctttattt
     2641 tcgacgatgg gctgtacgga ctggattttg acgaacccga agcgcaacac aatttccgcg
     2701 tcctctacta cgccgatccc gtgactgact ctgatgcgct catcaccgac tttacctttc
     2761 tgcgcagcta cggcgacgat atgccgctga aggcaggcag cgaatgggcc atgcgttttc
     2821 agccggatac cgggctgatg ccggagtcgg atttcaggtt tgacggcatt ttcggtgagg
     2881 acttttttgc tcgtttcggc actgatgagt ggagcatcgc cgaggcttac ggaaaaattt
     2941 ccgatgcagc cggccatcgc gtgggtggtt atgccgagtt cacgcaggaa gacccccggc
     3001 agccggaaga cctcatggaa ctgctcttcc agttggacac cgacagcaaa cacggcatcc
     3061 tctggggcga catgggcgtg gccaatttct tcattcgtcc tgaagatttg gcacagctca
     3121 acttctcccg cgttctttac aattgggact gctactgacc acactcaccc ctcacgcact
     3181 caccactgta cccctgcatc aggcctgcgc catgggcgta aaattcatcg gcggg
//
LOCUS       DAORLF010000497         3223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16346_length_3223_cov_2.165088, whole genome shotgun sequence.
ACCESSION   DAORLF010000497 DAORLF010000000
VERSION     DAORLF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16346_length_3223_cov_2.165088"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..>830)
                     /locus_tag="PK198_24665"
     CDS             complement(254..>830)
                     /locus_tag="PK198_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRF42018.1"
                     /translation="ADYVPVGKDQEQHLELSRNIAQRFNAQVGKEYFVLPEPLWAEIP
                     KVMSTAEPTRKMSASLGEKHNIDLFADEARIRKQIASAVTDTGPAQEGVMSAGVENLF
                     ALLRAAGRMEAHEALMENYRSGTLKYVDLKEAVAEGLVAISNPARARRAELLSDKKAV
                     KEQVKASSARIREQARQTVREVKELCGLMNV"
     assembly_gap    831..845
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<846..1279)
                     /locus_tag="PK198_24670"
     CDS             complement(<846..1279)
                     /locus_tag="PK198_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRF42019.1"
                     /translation="MENKRKQVLSAIQPTGVLHFGRYFGAISNWVRLQQEYDCIYGVV
                     DYHAMTMPFNPKKLREQSWDLIINLIAAGIKPENLFVQSLIPEHAELCWIFNCFTSYG
                     QLSRMTQFKDKSKQNQENAEDAFISAAFLDYPVLQAADILIYR"
     gene            complement(1313..1696)
                     /locus_tag="PK198_24675"
     CDS             complement(1313..1696)
                     /locus_tag="PK198_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF42020.1"
                     /translation="MKRIVRTDHAPAPIGPYNQAIIANNTLYASGQIALNPATGDLVT
                     GDIESETRQVMENIKAILEAAGLTFEHVVKVSVFVKDMHNFGRINGVYAEYFNDDTAP
                     ARELVEVANLPKFVNIEISVTAVLS"
     gene            complement(1696..1890)
                     /gene="rpmF"
                     /locus_tag="PK198_24680"
     CDS             complement(1696..1890)
                     /gene="rpmF"
                     /locus_tag="PK198_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRF42021.1"
                     /translation="MAHPKSRISKQRRNKRRTHHAAEMPQLATCKSTGEVHMYHRTYQ
                     DTDGNTFYRGKMLIKTADEA"
     gene            complement(1899..2468)
                     /locus_tag="PK198_24685"
     CDS             complement(1899..2468)
                     /locus_tag="PK198_24685"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRF42022.1"
                     /translation="MDALIQYLIPVKGLRHGVYDYNFHIDREFFSCFEASPVADGDVR
                     VHLRFDKQPSMFVLDLDIEGTVNTECDRCLAPIKLPIADQRSFVVKFSTEEESDDDDV
                     VFIHPDTQKFDVSPYVYEYVVLAIPMVKTYDCENDPTVECNREAFDRYILRTDEEEWP
                     EEPELEAPEDTENPVWNVLKQLNNDNNNN"
     assembly_gap    2666..2685
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2800..>3223)
                     /locus_tag="PK198_24690"
     CDS             complement(2800..>3223)
                     /locus_tag="PK198_24690"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRF42023.1"
                     /translation="IEDREGASVAWIFEEKGETYFRELERQTLHETSNRERAVISCGG
                     GTPCFFDNMDWMNAQGVTVWLDPPAELVLRRLQRKPHKRPLLAGLETDEQWLAFIAKK
                     MEERRPFYSRAQIVYRQEGEDANAAEELVNVLQSLGCE"
BASE COUNT          670 a          900 c          793 g          825 t
ORIGIN      
        1 tggccacaag ctacaagctg caaagcgcga gttgctgatg ggcgcggggt ttcgggtttc
       61 atccgccgta gccttggcga aggcgggttc gcgtttcagt tttccctctc gttcgcggga
      121 tttcgcgtgt ttcgcgttca aggtttcggg ttggagctgt gttgcctcgc attcgcggat
      181 tttcacatta ttcgctaaaa ttcgcgttct gtgtttcggg ttcgcgggta gaaaccgcct
      241 accgagaaac agctcacaca ttcatcaaac cgcacaactc cttcacctcc cgcaccgttt
      301 ggcgggcctg ttcacggatg cgcgccgacg aagcctttac ttgctctttt accgcctttt
      361 tatcagacag gagttcggcc ctgcgcgctc tggcaggatt gctgatggcc accagtccct
      421 ccgccacagc ttctttcagg tcaacgtatt tcagcgtgcc ggagcgatag ttctccatca
      481 gtgcttcgtg ggcctccatg cgaccggccg cccgaagcag ggcaaacaga ttttccacgc
      541 cggcgctcat cacgccttcc tgcgccggcc cggtatcggt cacggcagag gctatttgct
      601 tgcgaatgcg cgcctcatcg gcaaagaggt caatattgtg cttctcgccg aggctggcac
      661 tcattttgcg tgtgggttca gccgtggaca tcaccttggg gatttccgcc cacagcggtt
      721 ccggcagcac gaagtactcc ttgcccacct gcgcattgaa gcgctgcgca atgttgcgcg
      781 acaattccaa atgctgctcc tgatctttgc ccaccggcac ataatcggcc nnnnnnnnnn
      841 nnnnncggta aatcagaata tcggcagcct gcaaaacggg gtaatccaaa aaggccgccg
      901 agatgaaagc atcttccgcg ttttcctgat tctgtttgct cttgtcctta aattgcgtca
      961 tccggctcaa ctgcccatac gaagtgaagc agttgaaaat ccagcacaat tcagcgtgtt
     1021 cgggaatgag cgattggacg aagaggtttt ccggcttgat accggcagct atcaggttga
     1081 taatcaagtc ccaggattgc tcgcgcaact ttttggggtt gaagggcatc gtcatggcgt
     1141 ggtagtccac aacgccgtaa atgcagtcgt actcctgttg cagccgcacc cagttgctga
     1201 tggccccgaa atagcggcca aaatgcaaca cgccggtggg ctgaatggca gaaagtacct
     1261 gcttgcgctt gttttccatg actgaagctg cttggatttt gattttcgga gtttaggaca
     1321 aaactgccgt taccgaaatt tcaatattga cgaatttggg caagttggcc acttccacca
     1381 actcccgtgc aggagccgtg tcgtcgttga aatattcggc atacacgcca ttgatgcggc
     1441 caaagttgtg catgtctttc acaaacacgc tcaccttcac cacatgctcg aacgtcagcc
     1501 cggcagcctc cagaatggct ttgatgtttt ccatcacctg ccgcgtctcc gactcaatat
     1561 cgcccgtcac caagtcgccc gtagccggat tgagggcaat ctgacccgaa gcgtacaagg
     1621 tgttgttggc tataatggcc tgattataag gcccgatggg cgccggagcg tgatcggtgc
     1681 gaacaatgcg cttcatcagg cttcgtcggc agtcttgatc agcattttac cgcggtaaaa
     1741 cgtgttgccg tcggtgtcct gataagtgcg gtggtacatg tgtacttcgc cggttgattt
     1801 gcaggtggcc aattgaggca tttctgctgc gtggtgcgtt ctgcgcttgt tgcggcgctg
     1861 cttggagatt ctactcttag gatgtgccat tgtattgact aattgttgtt gttatcgttg
     1921 ttcaattgtt tcaaaacatt ccagaccgga ttctctgtat cttccggcgc ttccaattcc
     1981 ggctcctccg gccactcttc ctcgtctgtc ctcaaaatat agcggtcaaa agcctcccgg
     2041 ttgcattcta ccgtcgggtc attttcgcaa tcgtaggttt ttaccatcgg aatggccagc
     2101 acgacatact cgtacacata cggcgaaaca tcaaacttct gcgtgtcggg atgaataaaa
     2161 acgacatcat cgtcgtccga ttcctcttcc gtgctgaatt tcaccacaaa actgcgttga
     2221 tcggctatcg gcagcttaat gggcgccaaa cagcggtcgc attccgtgtt caccgtacct
     2281 tcgatgtcca aatcaaggac gaacatgctc ggttgtttgt caaatcgaag gtgtacccgc
     2341 acatctccgt ccgctaccgg cgaagcttca aaacacgaaa agaactccct gtcaatgtgg
     2401 aagttataat catagactcc gtggcgcaat cccttcacag ggatcaaata ttggatcagc
     2461 gcgtccatct tgccttcgtt ttaatactgc cctcctacga gagcgggtgc aaaggtaagg
     2521 tattgaaatt caattttgca aactttttgt gtgcaaaagt tttgtctcat cctacaaaaa
     2581 gagctttatt ttgataaaaa tgggggcgcc cccgcctcaa tcggctacca cgggttcagt
     2641 caggcagggt agcctcaggg cggcgnnnnn nnnnnnnnnn nnnnncatgg cggcctccag
     2701 tagagctgtt agctttatcg cgctattgca gccctaaagc taacagctct attcccgaca
     2761 gccgccattt cgtttccatc cctggcgcgg cgggccaact cactcgcatc caagcgattg
     2821 taacacattc actaattctt ccgccgcatt tgcatcttca ccctcttgcc tatatacgat
     2881 ctgcgcccga ctgtaaaacg gcctgcgctc ttccattttt ttagctataa atgccaacca
     2941 ctgctcatcg gtttctaagc cggccagcag cgggcgcttg tggggcttgc gttgcagacg
     3001 gcgcaacacc aattcagccg gcggatcgag ccataccgta acgccctgcg cattcatcca
     3061 gtccatattg tcgaaaaaac agggggttcc gccgccgcag gaaatgacgg ctcgctcccg
     3121 gttggaggtc tcgtgcaggg tttgcctttc caattcccga aaatatgtct cgcccttttc
     3181 ttcaaaaatc caagcaacac tagctccctc gcggtcctct atg
//
LOCUS       DAORLF010000498         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16396_length_3217_cov_2.247944, whole genome shotgun sequence.
ACCESSION   DAORLF010000498 DAORLF010000000
VERSION     DAORLF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16396_length_3217_cov_2.247944"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK198_24695"
     CDS             <1..307
                     /locus_tag="PK198_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42024.1"
                     /translation="EDAELIAGYDGVLFVDASKSVLFKGFEYQELEPALEFAFSTHAL
                     SPASVLALCRQVYERSPEAWLMAIGGEEWELQFGLSAAGGAHLKAALEFFFNRVGAA"
     gene            389..901
                     /locus_tag="PK198_24700"
     CDS             389..901
                     /locus_tag="PK198_24700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42025.1"
                     /translation="MSISIQYRGSMANTAEVETLMEEAEDIANIMRWPCEIWDEDWNR
                     PPDVHFESIRGGGIQIVGHSALRGISLQPHPLCEPLLLLFRPDGSMSSPFQLALDAGE
                     GYPQKQLWLSTETYSAGYLAHAAIVRLLQHLKKRYLHNLEIRDATGFADSGDETRLQA
                     FFHALSAVSG"
     gene            1536..2003
                     /locus_tag="PK198_24705"
     CDS             1536..2003
                     /locus_tag="PK198_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF42026.1"
                     /translation="MQVKRKEWQQWIPRPLNDVWHFFSRPENLNDLTPGDMSFQILSD
                     IKDTPMYEGMMICYKVSPFTGIQMDWVTEITHISEGRFFVDEQRFGPYSMWHHEHHFE
                     ARDGGVLMRDILHYKVPYGPIGTLADALFVDKKIEQIFGYRQKAVEEMFGTKP"
     gene            complement(2074..>3217)
                     /locus_tag="PK198_24710"
     CDS             complement(2074..>3217)
                     /locus_tag="PK198_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42027.1"
                     /translation="AYTYLSNLLGRTYEETVYAARRFDSDPTVNDLFRITYWLGRLSS
                     EEAHLKKDREITFSSALRERVGHHTYGDLWASHIKDYIVEKDMLDRPVYIVSANPHSF
                     MNALYAKAALPEQSKNKSLEELAGLLSQEDNGPLRDKVLAYALRHGMRQIDDTSGTNI
                     SVQIFDTTQVDAAQLPAELRWDAKRIQKEKPVLIVMDYAFGEQAYETMDELLKPCQTP
                     EGEVRFKVQSVSIMGKAGILEGEKGDIMVPTAHVFEGTADNYPLQNKFAKADFEGQGL
                     RVFEGPMITVLGTSLQNKDVLSYFLQSSWQAIGLEMEGAHYQKAIQSASRIRNSIAPD
                     VELRYAYYASDNPLLTGHTLASGSLGVEGVKPTYLITIKILNCIFG"
BASE COUNT          744 a          804 c          875 g          794 t
ORIGIN      
        1 agaggatgcc gagctgattg ctggttatga tggcgttttg tttgtggatg cctccaaaag
       61 tgtgttgttc aaagggtttg aatatcaaga gcttgagcct gcattggagt tcgccttcag
      121 cacgcacgct ttgtcgcccg catccgtttt ggctttgtgt cggcaggtat atgagcgcag
      181 ccccgaagcc tggctcatgg ccatcggcgg agaggagtgg gaactacagt ttgggctgag
      241 tgcagcaggg ggcgcgcatt tgaaagcagc gctggagttt tttttcaatc gggtcggcgc
      301 ggcatagtac aggggtaact taaatttatt ttttatcgtc cctttttgaa acaataccct
      361 atattcgctt tcgcacagcg acgattagat gagtatcagc atacagtacc ggggttcgat
      421 ggccaatacc gccgaggtgg aaacactcat ggaggaggca gaggacattg ctaatatcat
      481 gcgatggccc tgcgaaatct gggacgagga ctggaaccgg ccgccggatg tacattttga
      541 gagcattcgc ggaggcggca ttcagatcgt ggggcacagc gccttgcgcg gtatcagcct
      601 gcagccgcat cctttgtgcg agccgctgct gctgcttttt cggcccgacg gctccatgag
      661 ttcgcccttc caattggcat tggatgcagg cgagggctat cctcaaaaac agttatggct
      721 ctctaccgag acatatagtg cgggctatct ggcccatgcc gccatcgtca ggctgcttca
      781 gcatctcaaa aagcgctacc tccacaatct cgaaatccgc gatgccaccg gatttgccga
      841 ctccggcgac gagacccgct tgcaggcctt ttttcacgcg ctcagtgccg tatccggttg
      901 atataaaaaa gcggaggcta cgatcatcgc gcctccgcag aaatattcct gtatgagaaa
      961 cccttgcaat taagccggca tcgtcagtgc aacgccataa ggtaaaaagg gagcccccgc
     1021 tttgcttcag acaaccgcag ccaggccgca gccacatcgg cgctaccggc cccttcaacc
     1081 ggcagttgat cggggactcc ggtgacatct accctaacgt cgccaggtaa tgtgctggta
     1141 agcaggaatt tggacaaaaa ataggaatgt atcgtgttca tgggaatata catagctccg
     1201 tccggttcca gcctcgtcgg actggaaaag cgcgaatgca gatatgcctg acgcagcatg
     1261 gctcaggcag gaatcaacat tggaatcaat ggttggaagc gacggccggg aaaggccaat
     1321 gtatagaaaa tgaaaagcag gcggttagct ggcttgttca tgggaaaaca tgtttttggg
     1381 actaaaaata tcggtttcta catcgtatct ggtttcccag attacttttg caaagataca
     1441 gtcatatatc tcatcacaat ataccctttt cagggtattt tttgctgtaa acctttttat
     1501 ctggctcccg ttgtacctgc caaaatgatt cttctatgca agtaaagaga aaagagtggc
     1561 agcaatggat acctcgcccg ctgaacgatg tatggcattt tttttcccga ccggagaacc
     1621 tcaacgacct gacgccgggt gatatgtcgt ttcaaatttt gtcggacatc aaagacacgc
     1681 ccatgtacga gggcatgatg atttgttaca aagtcagccc gtttaccggc attcagatgg
     1741 actgggtgac ggaaataacg cacatctcgg aagggcgctt ttttgtggac gagcagcgct
     1801 ttggccctta ctcgatgtgg caccacgagc atcacttcga agcgcgcgac ggcggcgtct
     1861 tgatgcgcga catccttcac tacaaagtac catacggccc catcggcacg ctggcagacg
     1921 cgctgtttgt ggacaaaaaa atagagcaaa tattcggcta ccggcaaaag gcggtggaag
     1981 agatgtttgg cacaaagcca tagtagtggg gcaaaaataa agtgtttatt tttggcgaaa
     2041 atgatcactt tattttttta tcgccccata ggttcaacca aaaatacagt tcaaaatttt
     2101 gatcgtaatg agataagtag gcttcacgcc ttccacgccg aggctgcccg atgccagcgt
     2161 atggccggtg agcagcgggt tgtctgaggc gtagtaggcg tagcgaagtt ccacatcggg
     2221 cgcaatgctg ttgcggatgc gggaagccga ttggatggcc ttttgatagt gcgcgccctc
     2281 catttccaac ccgatggcct gccaggaaga ctggagaaaa taactcagta catctttgtt
     2341 ttgtaacgaa gtgcccaaaa cggtaatcat cgggccttca aacacgcgca ggccctgacc
     2401 ttcaaaatcg gctttcgcaa atttgttttg cagcgggtag ttgtctgctg tgccttcaaa
     2461 aacatgggcg gtaggcacca tgatatcgcc tttttcgccc tccagaatac cggctttgcc
     2521 cataatactg accgattgca ccttgaaacg cacctcgcct tccggggtct ggcaaggctt
     2581 gagcagctcg tccatcgttt cgtaggcctg ctctccgaag gcataatcca ttacaatcag
     2641 taccggtttt tccttttgta tgcgtttggc atcccaacgg agttctgccg gcaattgagc
     2701 ggcatcaacc tgagtggtat caaaaatctg aacggaaata ttggtgccgc tggtgtcgtc
     2761 tatctggcgc atgccgtggc gcagggcgta ggccagcact ttgtcgcgca gcgggccgtt
     2821 atcttcctgg ctgaggaggc cggccaactc ttccagactc ttgtttttgc tttgttccgg
     2881 cagtgctgcc ttggcgtaga gcgcgttcat aaagctgtgc gggttggcgc tcacgatgta
     2941 taccggacgg tcgagcatat ctttttcgac gatataatct ttgatgtgcg atgcccagag
     3001 gtcgccgtaa gtatggtgcc ccacgcgttc gcgcaaagca gaggaaaagg tgatttcccg
     3061 gtcttttttg aggtgagcct cctccgatga caggcgcccc agccagtaag tgattcggaa
     3121 gaggtcgttc accgttgggt cgctatcgaa gcggcgggcg gcatacacgg tttcttcata
     3181 ggtgcgcccc aacaggttgc tcagataggt gtaggcc
//
LOCUS       DAORLF010000499         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16411_length_3215_cov_1.993038, whole genome shotgun sequence.
ACCESSION   DAORLF010000499 DAORLF010000000
VERSION     DAORLF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16411_length_3215_cov_1.993038"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..>801)
                     /locus_tag="PK198_24715"
     CDS             complement(50..>801)
                     /locus_tag="PK198_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42028.1"
                     /translation="DEPLPPPEKDTPTPSNTSRPGRLWIAALLGAVVVLAAVAWRMDW
                     LGYKSESVPTEKTPSEKENSNQPTPGPIAGIDEPTEPEKSTTLPNPEIRPAPQSPPAD
                     GGMMAYAGKLYEDLDLDNTRKGAPMEEDEGPLSEAVIAYKKEQYHQALQLLNAVSEGN
                     GYAIRALEMKAHTLYHLKKYKESAAAFAAVAATELPPFAERAQWNQLVCYTAQYPATK
                     AAFDELLAVLIADTGHTYHERALELQKRLSE"
     assembly_gap    802..844
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1014..1628)
                     /locus_tag="PK198_24720"
     CDS             complement(1014..1628)
                     /locus_tag="PK198_24720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF42029.1"
                     /translation="MNRDDASIIAGIRGTAEERDAALAFFFRQHRSLRAQVIAYVAKS
                     GGTEHDGEDVFQDAVVLFDRRIRKGAFKEESSLSTYFFSIAKFHWIGQHRRRKIMEEL
                     PDRRDEKGDVGPELDYIFEERKQIFYSALEQIGKKCKEVLGFYMLDYKMEEIAQITGR
                     GSAAMAKKDAYKCRMKLRAYLLQRPQLLSALGVQLPDNPDLLTE"
     gene            complement(1704..2429)
                     /locus_tag="PK198_24725"
     CDS             complement(1704..2429)
                     /locus_tag="PK198_24725"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRF42030.1"
                     /translation="MHTDSVRRAGSLSLGLSFTENNGKQLFVTRNALALQSKSKSLRD
                     IWLVLANLDLSKADGAAFSNSAFLHLRYNRKMSGLLRWEAFTQAQYNRVLGIGSRWLA
                     GTGPRLKLFSTEKSALYWGNLYMFEYEATTANLPVISRQHRLSAYLSATFDLSPTLPG
                     ELITTTYYQPRLNRIADYRLSHETSLELNITKKLRATTRLYYFYDAVPPSGIAGRALA
                     VEQGFRVGFEWHFKADYFTDIFK"
     gene            complement(2516..>2860)
                     /locus_tag="PK198_24730"
     CDS             complement(2516..>2860)
                     /locus_tag="PK198_24730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF42031.1"
                     /translation="WFTRCPPCVAEMPHLNALRKDFAGEDVVFLAITPDDTATVQAFL
                     KKRPFDFTILPDAQAYINFFGNEYPLNIFVDKQGIIRHARAAIPTSFDQRHPQGFMDD
                     REFRRMLGNLLR"
     assembly_gap    2861..2880
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2881..3096)
                     /locus_tag="PK198_24735"
     CDS             complement(<2881..3096)
                     /locus_tag="PK198_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42032.1"
                     /translation="MKFLKTDTYLLGLACCLLLLSLSACRQAPASVVETPEDYPKLVG
                     QEFPDFELRNLDGQLLRWSDLKGKAALV"
BASE COUNT          691 a          816 c          858 g          787 t
ORIGIN      
        1 cccgtactac tgggccgggt ttatcggcgt gggggatatg tagccgaatt tattcagaca
       61 accgtttttg tagttctaac gctctctcgt ggtaagtatg ccccgtatcc gctatcagca
      121 ccgccaacaa ctcatcgaat gctgctttgg tagccgggta ttgagccgtg tagcacacca
      181 attggttcca ctgtgcgcgc tccgcaaacg gaggcaattc tgtagcggcc acggcggcaa
      241 aagcagcagc gctttcttta tattttttga gatggtacaa ggtatgggct ttcatttcta
      301 aagcacgaat tgcatagccg ttcccttcgg acactgcatt caataattgt aatgcctggt
      361 ggtactgttc tttcttgtat gcaattactg cttccgaaag cgggccttca tcttcttcca
      421 taggcgcccc tttcctggta ttatccaagt ccaaatcttc atacaacttg cccgcgtaag
      481 ccatcatgcc gccatcagcc ggagggcttt gaggggcagg gcggatttcg gggttcggaa
      541 gagttgtgga tttttcaggc tcggttggtt catctatacc tgcaatgggg ccgggagttg
      601 gttgatttga gttttctttt tcggagggcg ttttttcggt gggaaccgat tcactcttat
      661 accccaacca atccatgcgc caagccactg ccgccaaaac gacaacagca cccaagagcg
      721 cagctatcca aagccggccg gggcgcgaag tgttcgaggg tgtgggggtg tctttttccg
      781 gaggaggcag cggctcgtct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnncatggc ctccatagcc tccatctcta cttgttgcaa ttggaactcc agcgcggcct
      901 ccgggttggt ttgtatttcc cgctccaccg cagccctttc ctcttcggag agccggcctt
      961 tgaggtaggc ttctatttta tcgtatagtt cttcgttgtt catgtcattg gcattattcg
     1021 gtgagcaagt cggggttgtc aggcaattga acgcccagag cagacagcaa ttgcggcctt
     1081 tgcagcaaat atgcccgtag tttcatccgg catttgtagg catctttttt ggccattgcc
     1141 gcactgcccc ggcctgtaat ttgggcaatt tcttccattt tgtagtccaa catgtaaaag
     1201 cccagcactt ctttacattt tttcccgatc tgctctaaag ccgaataaaa aatctgcttc
     1261 cgctcttcga aaatatagtc cagttccggc ccgacatctc ctttttcatc ccgacgatcc
     1321 ggcagctctt ccatgatttt tcggcggcgg tgttgcccga tccaatggaa tttggcaatg
     1381 gaaaaaaaat aagtactgag tgaactttcc tccttgaacg cgccctttct gatgcgtcgg
     1441 tcaaacaata ccaccgcatc ttgaaataca tcttctccgt catgctccgt accgccggat
     1501 ttggccacat aagcaatcac ttgagcgcgc aaacttcggt gttgcctgaa aaaaaaggcc
     1561 agtgcggcgt ctcgctcctc ggcggtgccc cggatgccgg ctatgatgga cgcgtcgtct
     1621 cgattcattg gcttagtgac attgttggat aaaaggtgac cagtagatgc aaaaataatt
     1681 gaattaagca gtttgtatcc cactcattta aaaatatcgg taaaataatc tgctttaaag
     1741 tgccattcaa accccacccg aaaaccctgc tccaccgcca gcgcccggcc tgcgatgcca
     1801 cttggcggaa ctgcatcgta gaaataatac agccgcgtgg tggcccgcag ttttttggtg
     1861 atgttgagtt cgaggctggt ttcgtggctc aggcggtagt cggctatgcg gttgaggcgg
     1921 ggttggtagt aagtggtcgt aatcaattcg ccgggcaacg tgggactcag atcaaaggtg
     1981 gccgagaggt atgcactgag gcggtgctgc cggctgatga cgggcaaatt ggccgtagtt
     2041 gcttcatatt caaacatgta tagattgccc cagtacaggg cgcttttttc tgtggagaac
     2101 aacttgaggc gcggccctgt accggccagc caacgggagc cgatgcccag cacccggttg
     2161 tactgcgcct gtgtgaaggc ttcccagcgc aacaggccgc tcatttttcg attgtagcgc
     2221 agatgcagaa acgccgaatt gctgaacgcg gcgccgtcgg ctttcgacag gtctaagttg
     2281 gccagcacga gccagatgtc gcgcaggctt ttcgatttgg attgcagcgc cagcgcattg
     2341 cgcgtcacga agagttgttt gccgttgttt tcggtgaacg acaagcccag cgagaggctg
     2401 ccggccctgc gaaccgagtc ggtgtgcata cggcgatttt cgatgttgac cacctgcgcg
     2461 cgaagggcgg cggagcaaga taaaaacatg ataagcaata ggaaaagtcg catcatcatc
     2521 ggaggagatt gcccagcata cgccgaaatt cccggtcgtc cataaagccc tgcggatgcc
     2581 gctgatcgaa gctggtagga atggcggcgc gcgcatgacg gatgatgcct tgtttgtcaa
     2641 caaaaatatt gagcgggtac tcgttgccga aaaaattgat gtacgcctgc gcatcgggga
     2701 ggatggtaaa atcgaagggg cgttttttca aaaaagcctg cacggtggcc gtatcgtcgg
     2761 gggtgatggc gaggaatacc acgtcttcgc cggcaaaatc cttgcgcaaa gcattcagat
     2821 gcggcatttc ggccacacag ggcgggcagc gggtaaacca nnnnnnnnnn nnnnnnnnnn
     2881 gaccaaggcg gccttgcctt tgaggtcgct ccaacgcagc agttggccgt caaggttgcg
     2941 cagttcaaag tcgggaaact cctgccccac aagtttggga tagtcttcgg gcgtttccac
     3001 cacagaggca ggcgcttgcc ggcaggcgga gagactgagc aaaagcagac agcaggcgag
     3061 gccgagtaga taagtatcag tttttagaaa tttcatcagg ttcaatagtt gaataggtac
     3121 ctgttactct gctgcccgta cctcctgctc cgacagccct gtttcaagcg ccgcatcttt
     3181 gacggacata cccgtgcgca gcagcaggcg ggcta
//
LOCUS       DAORLF010000500         3205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16484_length_3205_cov_1.887302, whole genome shotgun sequence.
ACCESSION   DAORLF010000500 DAORLF010000000
VERSION     DAORLF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16484_length_3205_cov_1.887302"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..614
                     /locus_tag="PK198_24740"
     CDS             321..614
                     /locus_tag="PK198_24740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF42033.1"
                     /translation="MPNQANQGRIDFLVEEGVARIRFYHPAHNSMPGYLLSELTQAIE
                     QAGNNDAVRVLVLESEGDRTFCAGASFDELAAIEAEIVEEVEALRAALGEVGT"
     assembly_gap    617..626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <627..1127
                     /locus_tag="PK198_24745"
     CDS             <627..1127
                     /locus_tag="PK198_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF42034.1"
                     /translation="NVINAMRRCPKFIIGRVQGKAIGGGVGLASATDYCFATEAAAVK
                     LSELAVGIGPFVVGPAVERKVGKAAFAQLAIHATEWQTAQWAKEKGLFQEVFSDIAAM
                     GAHIDQLCKRLAASHPEAMCRLKSVFWEDAAHWDQLLDERAAVSGQLVLSGYAKNAIQ
                     AFKQKS"
     gene            1129..1383
                     /locus_tag="PK198_24750"
     CDS             1129..1383
                     /locus_tag="PK198_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42035.1"
                     /translation="MNPNEILKKHFLATVDEQLASGEPPQTAETMQRLQSDGYSEKNA
                     RLLVAQCVALEYVQSIETDLPFDYERFVRNLSNLPAQPVV"
     gene            1433..>2317
                     /locus_tag="PK198_24755"
     CDS             1433..>2317
                     /locus_tag="PK198_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRF42036.1"
                     /translation="MMTAAQTNTKVLTKAFRLMATAYWMAQVYEENFKFASKYVHATS
                     RGHEAVQLATAFHLRPQDFAYPYYRDEAMLLGIGMRPYDLMLQLMAKREDPFSGGRTY
                     YVHPSLRDADKPQIPHQSSATGMQAIPATGAAMGLWYKASHQMADWKPADAPIVLCSF
                     GDASITEGEVAEAFQMAALKQLPILYMVQDNGWDISANAAETRAQNAAEYARGFHGIE
                     TRSIDGSDFAQCYEVIGEVVETIRRERRPFLIHASVPLLNHHTSGVRKEWYRDDLEAH
                     QLRDPYPLLRRLMLEEGWS"
     assembly_gap    2318..2332
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2333..>2698
                     /locus_tag="PK198_24760"
     CDS             <2333..>2698
                     /locus_tag="PK198_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tungsten formylmethanofuran dehydrogenase"
                     /protein_id="HRF42037.1"
                     /translation="LQQIEAEAEALVRADFAAARQAEDPRPEDLFTHDFAPTPVTEER
                     GEREPEGNEPKVMVDCALFALEELLEQHPECLIYGQDVGARLGGVFRECATLAQKFGS
                     HRVFNTPIMEAFIIGSTAG"
     assembly_gap    2699..2721
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2722..>3205
                     /locus_tag="PK198_24765"
     CDS             <2722..>3205
                     /locus_tag="PK198_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten formylmethanofuran dehydrogenase"
                     /protein_id="HRF42038.1"
                     /translation="RPIVEVQFADYIWPGLNQLFTELSRSCYLSNGKWPVSAILRVPI
                     GAYGSGGPYHSSSVESVVTNIRGIKIAYPSNGADLKGLLKAAWYDPNPVVIFEHKGLY
                     WSKVPGTETARTIEPSADYVVPFGKARLALEASQEAVKSGESLLVVTYGMGVHWALNA
                     A"
BASE COUNT          741 a          917 c          832 g          667 t
ORIGIN      
        1 cttatctaat cctttggcct aaagaaaatg aacatttatt tttgccccac tactaagagc
       61 ggtgaaaaaa gtcattctca ggctgctgtc tttgatgtaa atttgcgatc aggtaattta
      121 cagtcgcctg ctaaaagctg tgagccgcga gtctggtttt ccaaaagaca aggtattcgt
      181 tttcaatcgt aagttttgaa accttcctga atggaaaatc taattattca acgaataaac
      241 aaactcgtac cacgccgggg gcgtggtcat agctcgcagc ttgagatagt tatgtgatca
      301 gatttatatc atcctgacca atgcccaacc aagccaatca aggccgcata gactttctcg
      361 ttgaggaagg cgtggcccgt atccgatttt accatcccgc ccacaactcc atgcccggtt
      421 atttgctctc ggagttgacg caagccattg aacaagccgg aaataacgac gctgttcggg
      481 tactcgtact cgaaagtgag ggagatcgca ccttttgcgc gggcgccagt tttgacgaat
      541 tggccgccat cgaggccgag attgtggaag aggtggaggc gctgcgggcg gcgttgggcg
      601 aggtcggaac atgactnnnn nnnnnnaatg tcatcaacgc catgcgccgc tgccccaaat
      661 tcatcatcgg cagggtgcag ggcaaagcca tcggcggcgg ggtaggactg gcttccgcta
      721 cggactactg cttcgctacc gaggccgccg cagtgaaact cagcgaactg gcagtcggaa
      781 taggtccttt tgtagtcggc ccggcggtag aacgcaaagt gggaaaagcg gcttttgccc
      841 aattggccat ccatgcaacc gaatggcaaa ccgcccaatg ggccaaggaa aaaggtttgt
      901 ttcaggaggt tttttccgac attgccgcga tgggcgcgca cattgaccaa ctttgtaaac
      961 gcctcgccgc atcacatccc gaagccatgt gccgcctcaa atctgttttt tgggaagacg
     1021 ccgcccattg ggatcagttg ctcgacgaac gcgctgccgt cagcgggcaa ttggtgctgt
     1081 ccggctacgc caaaaatgcc attcaggcgt tcaaacaaaa aagctgatat gaatccgaac
     1141 gaaatactca aaaagcattt tttggcaacg gtggacgaac aactcgcatc gggcgaaccg
     1201 ccgcaaaccg ccgaaaccat gcaacgcctg caatccgacg gctacagcga aaagaacgct
     1261 cgtttgctcg tggcccaatg tgtggcgctg gagtatgtgc aatccatcga aaccgatctt
     1321 ccttttgatt atgagcgatt tgtcagaaat ctgtcgaatc tgccggccca acctgtggtg
     1381 tgacgagcgg cgcacagttg gtcgtcttta ccgtttaaaa ccaaataaaa ccatgatgac
     1441 tgctgcccaa accaatacca aagtcctgac caaagccttc cgcctcatgg ctaccgccta
     1501 ctggatggct caggtgtatg aagaaaactt caaattcgct tccaaatacg ttcacgccac
     1561 ctcgcgcggc cacgaggcgg ttcagttggc tactgccttc cacctgcgcc cacaggattt
     1621 tgcctatcct tattaccggg atgaagccat gctgctcggc atcgggatgc gcccttacga
     1681 cctgatgctc caactcatgg ccaaacgcga ggacccattc tccggcggcc gtacttacta
     1741 cgttcatccc agcctccgcg atgccgacaa accccaaatc ccacaccagt cttcggccac
     1801 cggtatgcag gccattccgg ccaccggagc agctatgggc ctgtggtaca aagcctccca
     1861 ccaaatggcc gattggaaac cggcagatgc gcccattgtg ttgtgctctt tcggcgatgc
     1921 cagcatcacg gaaggcgaag tggccgaggc tttccaaatg gcggcgctca aacaactgcc
     1981 tatattatat atggtgcaag acaatggctg ggacatttcc gccaatgccg ccgaaacccg
     2041 cgctcaaaat gccgccgaat acgcacgggg ctttcatggc atcgaaaccc gcagcataga
     2101 cggcagcgat ttcgcgcagt gctatgaggt catcggcgag gtggtggaaa ccatccgtcg
     2161 cgaacgccgc cccttcctca ttcacgcatc cgtacccctg ctcaatcacc acacctccgg
     2221 cgtgcgcaaa gagtggtacc gcgacgatct ggaggcacat caattgcgcg atccctaccc
     2281 gctgctccgc cggctcatgc tggaagaagg ctggtcgnnn nnnnnnnnnn nntttgcagc
     2341 aaatcgaagc cgaggccgag gcattggtca gagccgattt cgccgccgcc cgccaggccg
     2401 aagacccgcg ccccgaagac ttgtttacgc acgattttgc ccctactccc gtcaccgaag
     2461 agcgcggcga acgcgaacca gagggcaacg aacccaaagt aatggtggac tgtgcgctct
     2521 tcgccctcga agaactgctc gaacagcatc ccgaatgtct tatctatggt caggatgtgg
     2581 gcgccaggct gggcggcgtt ttccgcgagt gtgccacgct ggcccaaaaa ttcggcagtc
     2641 atcgagtttt caacacgccc atcatggagg cattcatcat cggcagcacg gcaggcatnn
     2701 nnnnnnnnnn nnnnnnnnnn ncgcccgatt gtggaagtgc agtttgccga ttacatctgg
     2761 ccgggcctca atcagttgtt caccgagctg agccgcagtt gctacctttc caacggaaag
     2821 tggcccgtgt cggccatctt gcgggtaccc atcggcgctt acggcagcgg cggcccgtac
     2881 cactcctcca gcgtcgagtc ggtggtgacc aatatccggg gtatcaaaat agcttacccc
     2941 agcaatggcg ccgacctcaa aggtctgctc aaagctgcct ggtacgatcc caatccggtg
     3001 gttatttttg aacacaaagg actgtactgg agcaaagtgc ccggcaccga aaccgcccga
     3061 accatcgaac cctccgccga ttatgtggtg cctttcggca aagcgcgact ggcgttggaa
     3121 gcttcgcaag aggcggtgaa aagcggtgaa tcattactcg tggtcaccta tggcatgggc
     3181 gtgcattggg cgctcaatgc tgcca
//
LOCUS       DAORLF010000501         3202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16510_length_3202_cov_2.218303, whole genome shotgun sequence.
ACCESSION   DAORLF010000501 DAORLF010000000
VERSION     DAORLF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16510_length_3202_cov_2.218303"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PK198_24770"
     CDS             complement(<1..134)
                     /locus_tag="PK198_24770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF42039.1"
                     /translation="MISLILGGGAGSRLHPLTSQRSKPAVPIGGKYRLIEIPISNCLN
                     S"
     gene            complement(175..1614)
                     /locus_tag="PK198_24775"
     CDS             complement(175..1614)
                     /locus_tag="PK198_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRF42040.1"
                     /translation="MKILHISAECYPAAKAGGLGDVVGALPKYLRTAGASAAVIIPKY
                     RVKWINNHQFEPVYQGVVRIHHGVVPFQIQREVGDTLGFPLFVAEIPGKFDRPGIYND
                     PGGWGYGDEVERYLSFQQAVLQWVAHSPGRPDVLHCHDHHTGLIPFMVKHCPEYRSLS
                     RIPTVFTIHNGAYHGAFSWEKFYLLPLFDADARGLLDWANTISPLAAAVKCAWRVTTV
                     SPSYLEELRYDSNGMEALFWHEQHKCVGILNGIDAQVWNPANDPYIASQLKDGNIDAF
                     KGENKAALQQHFNFDAQPPVISFIGRLVMEKGADLIPDLIRKAMYSGMRASFLILGTG
                     EGRIERDFDRLRNEFRGRVDARLEYNEGVAHQMYAGSDFLLMPSRVEPCGLNQMYAMR
                     YGTMPIVRKVGGLKDSVPDIGEPNGLGRGIQFTHFDVDDAFKALYRGLETIFFHLEHF
                     NKTRARNMQLDFSWERAAHNYIEIYRQLM"
     gene            complement(1652..1987)
                     /locus_tag="PK198_24780"
     CDS             complement(1652..1987)
                     /locus_tag="PK198_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42041.1"
                     /translation="MKKYVWIGMLALVATACQPEDPIVKMADDVCACLMPTVEQGDKA
                     TEAIKRNNEAELAALEAEMLKTQEETQACFQNLEKKYGNMQEFQARVTEAMKKRCPRA
                     AKLISNPGE"
     gene            complement(2078..3100)
                     /locus_tag="PK198_24785"
     CDS             complement(2078..3100)
                     /locus_tag="PK198_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42042.1"
                     /translation="MKYFVALLLIGVFCFSLASSRSVTPASADAGKKYGLSFVAPPDS
                     FSTEAMEAVRHAGAGWIAAIPYAFTRVGEPRVYYNSGRQWWGERPEGCRHTIRLAHEA
                     GLRVMLKPQVYVPRGWVGSLDFDTEEAWQSWENDYEAYILPFAEMAEELNVALFCIGT
                     EFKTAVPKREAFWRRLIAKVRQRYSGPLTYAANWDEYAKVPFWDALDYIGVDAYFPLS
                     EAATPSVNELAGHWQPWVEQLRDFSHKTQLPILFTEYGYLSVDGCAGKTWELEKKLPE
                     LAPNQQAQANAIEALLLVFSREDFWAGGFLWKWYPTRRGREDQYGKDYTPQGKTALEI
                     LRSRYR"
BASE COUNT          729 a          854 c          865 g          754 t
ORIGIN      
        1 gaattgaggc agttggagat gggaatttca atgagccggt acttgccgcc tatgggtacg
       61 gccggtttgg aacgttggct ggtaagcggg tgcaatcggg agccggcgcc gccgccgagg
      121 atgagactga tcatttttat catgacgaag tgcgttttta tgatggttgg tttttcacat
      181 cagttgccga taaatctcta tataattgtg tgcagcgcgt tcccacgaga agtctagctg
      241 catgttgcgc gcccgcgttt tgttgaaatg ctccaagtgg aaaaagatcg tttccagccc
      301 ccggtacagc gccttgaagg cgtcgtccac atcgaaatgc gtgaactgaa taccgcgccc
      361 cagcccgttg ggttcgccga tgtcgggtac cgaatctttg agaccgccca ccttccgcac
      421 aatgggcatg gtgccgtatc gcatggcgta catttggttg agaccgcaag gctctacccg
      481 cgagggcatg agcaggaaat cggagccggc atacatctgg tgggccacgc cttcattgta
      541 ttccaaacgg gcatccacgc ggccccggaa ctcattgcga agccggtcga agtcgcgttc
      601 gatgcgtcct tcgccggtgc cgagtatcag gaaggaagct ctcatgccac tgtacatggc
      661 ctttcggatg agatcgggaa tcaggtcggc gcctttttcc atcaccaaac ggccaataaa
      721 actgatgacc ggcggctgcg catcgaagtt gaaatgctgt tgcagggccg ctttattttc
      781 gcccttgaag gcgtctatat tgccgtcttt gagttgcgag gcgatgtatg gatcgttggc
      841 cgggttccaa acctgcgcat ctatgccgtt gagaataccc acgcatttgt gctgttcgtg
      901 ccaaaacagg gcctccatac cattggaatc atagcgcaac tcttccagat aactgggcga
      961 aacagtggtg acgcgccagg cacatttcac ggctgccgcc aatggactga tggtattggc
     1021 ccagtctaaa agaccgcggg catcggcgtc gaacagcgga agcaggtaaa atttctccca
     1081 actgaatgca ccgtgatagg cgccgttgtg aatggtaaaa acggtaggaa tacggctgag
     1141 cgagcggtat tcggggcagt gcttgaccat gaacggaatg agaccggtat ggtggtcgtg
     1201 gcaatgcagc acatcgggcc gccccggcga atgagctacc cactgcaaca ccgcctgctg
     1261 gaacgagaga taccgctcta cttcatcgcc gtagccccag ccgccgggat cgttgtagat
     1321 gccgggcctg tcgaacttgc ctggaatctc ggccacaaaa agcggaaaac ccagagtatc
     1381 gcctacttcg cgttgaatct gaaacggcac caccccgtga tggatgcgca ccacaccctg
     1441 atagaccggc tcaaactggt ggttgttgat ccatttgacg cggtatttgg gaatgatgac
     1501 ggccgcagaa gcgcctgcgg tgcgaaggta tttgggcaat gcgcccacca cgtcgcccag
     1561 accgcctgct ttggcggcag gataacattc ggcgctgatg tgtagtattt tcatactcgg
     1621 aagattatgg tgaggtgtca ggcagaaggc cctattcgcc tggatttgaa ataagtttgg
     1681 ctgctctggg gcaacgtttt ttcatggctt cggtaacgcg tgcctgaaac tcctgcatgt
     1741 tgccgtattt tttctccaga ttttggaaac aggcctgggt ttcttcctgc gttttcagca
     1801 tttccgcctc aagagcggcc aattcggctt cgttgttacg cttgatggcc tctgtagcct
     1861 tgtcgccttg ttctaccgta ggcatcagac aggcacatac atcgtcggcc atttttacaa
     1921 tcgggtcttc aggttgacag gctgttgcga ccagcgccag catcccgatc catacatatt
     1981 ttttcatcac tttcaggttg catttagatt ggctccaaat gtaacacatc tgttttaatt
     2041 accaataaac ggaaaataaa atctgtaaaa actcagctca ccggtacctg ctgcggagta
     2101 tttcaagcgc tgttttgcct tgcggagtgt agtctttgcc gtactgatct tcacgacctc
     2161 tgcgagtggg ataccatttc cacaaaaagc cccctgccca aaagtcttcc cgtgagaaaa
     2221 caagcagcag cgcttctatg gcattggcct gcgcctgttg attgggagcc agttcgggca
     2281 gctttttttc caactcccag gttttacctg cgcagccgtc cacactcagg tagccatact
     2341 ccgtaaataa tataggcaac tgtgttttgt gagaaaagtc tctgagttgt tctacccagg
     2401 gttgccagtg cccggccagt tcattcaccg atggcgtggc ggcttcggaa agcgggaagt
     2461 aggcatctac gcctatataa tcaagggcgt cccaaaacgg gactttggca tactcgtccc
     2521 agttggcagc ataagtgagc gggccgctgt atcgttggcg caccttggcg atcaacctgc
     2581 gccagaaagc ctcgcgtttc ggtacggccg ttttgaattc cgtaccgatg caaaacaacg
     2641 caacattcag ttcctccgcc atctccgcaa aaggcagaat ataggcttcg tagtcatttt
     2701 cccaagattg ccaagcctct tctgtgtcga aatcgagcga accgacccag ccacgcggca
     2761 catacacctg cggtttgagc atgacgcgca atccggcctc gtgcgccaag cgtatggtat
     2821 gccggcagcc ttcgggccgc tcaccccacc actggcgccc cgaattgtaa taaacccttg
     2881 gctcgcccac gcgggtaaag gcataaggaa tagcggctat ccaaccggcg ccggcatgac
     2941 gcactgcttc catcgcctct gttgaaaaag aatcgggcgg agctacgaag ctcagaccgt
     3001 actttttgcc ggcatcggcg gaggccggag taacagagcg cgacgaggct aatgaaaagc
     3061 aaaacactcc gatgaggagc agggctacaa agtatttcat ggtacaaatt tgggtgtaaa
     3121 cggtgtaaaa cttcagcgta caatcatcaa tggttcgtga tatactcacg ccgcccaggc
     3181 ggcgtaataa ttagaaaata ag
//
LOCUS       DAORLF010000502         3200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16521_length_3200_cov_1.783466, whole genome shotgun sequence.
ACCESSION   DAORLF010000502 DAORLF010000000
VERSION     DAORLF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_3200_cov_1.783466"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK198_24790"
     CDS             <1..1054
                     /locus_tag="PK198_24790"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF42043.1"
                     /translation="GASPEGNRPFIDQMEVATKQTKRLWRSEAPWYETPVAFVDLGKG
                     QVITRRESVQEPPNFYLRNLNKNELKPLTKFANPYSALTGAKKELMRYKRADGVEMTG
                     TLYLPAGYDKAKDGPLPTLMWAYPREFKSADAAGQVSGSPYEFIRLSWGSPLYWLTQG
                     YAVFDDFSMPIIGEGDAEPNETFVEQLRSNAEAAVNMLVNMGVTDRRRIAVGGHSYGA
                     FMTANLLAHTDLFAAGIARSGAYNRTLTPFGFQSEERTLWQAPEVYSNMSPFNFADKI
                     KTPILLIHGEADNNSGTFPIQSERFYAALKGHGATVRYVVLPAESHGYRAKESVLHCL
                     WEMDQWLDKHVKNRKG"
     assembly_gap    1256..1269
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1270..2200
                     /locus_tag="PK198_24795"
     CDS             <1270..2200
                     /locus_tag="PK198_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42044.1"
                     /translation="IYHQNARLMERLLGLPALHLGARILQSLLLLAEICVLILLAAAA
                     LIRYRKPRVFISFKYLHEEKAKEIGRQVERAGMEVLRLPFGSYGHDEIVEFVRVALRK
                     ADAVVVIPDAESASFVDSELLAASVRRIPIALIQYQERQFQPRTLLRGYPVFDYACLE
                     AGGFESLNRYLWFSAGHPREYVRMAGRIFAVFLEPVNWIVFSLMLVAMALMEGIKWLI
                     NAASYALLQTAIFQPDMESAVFYNTVFVMLLLGYALYLLYRQWNVLRTARQIASTGTE
                     SYQVFAEAFSILKSDQKILACIRENLFIPRGKG"
     gene            complement(2300..>2937)
                     /locus_tag="PK198_24800"
     CDS             complement(2300..>2937)
                     /locus_tag="PK198_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF42045.1"
                     /translation="WLEQGGIYVSANIRGGSEYGEAWHEAGMRLKKQNVYDDFIAAAE
                     YLIANKYTSTKYLACQGGSNGGLLVGAVINQRPDLFGVAIPQVGVMDMLRFQKFTIGW
                     NWIAEYGSAEATEEDFKNLYAYSPLHNIKAGTSYPNVLITTADHDDRVVPAHSFKYAA
                     ELQSKARTDRPALIRIETESGHGASNLKKSLEVVADMYAFCFYNMGLTPKF"
     assembly_gap    2938..2950
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2951..>3200)
                     /locus_tag="PK198_24805"
     CDS             complement(<2951..>3200)
                     /locus_tag="PK198_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF42046.1"
                     /translation="FAYPPSIYTYDIATGKSTLFRAPEVDFNPDAYETKQVFYTSKDG
                     AKVPMFIIHKKGLELNGKNPTILYAYGGFNVSVTPSFS"
BASE COUNT          671 a          798 c          926 g          778 t
ORIGIN      
        1 gggtgcatcg ccggaaggca accgtccttt tattgatcag atggaggtag caaccaagca
       61 aaccaagcgt ttgtggcgtt cggaagcacc ctggtacgaa acgccggtgg cttttgtgga
      121 cttgggcaag ggccaggtca ttacgcggcg ggaatcggtg caggaacccc ccaattttta
      181 tctgcgcaat ctgaacaaaa acgaactcaa acccctgacg aaatttgcca acccttactc
      241 ggctctgacc ggcgccaaaa aagaactgat gcggtacaaa cgcgccgacg gggtggaaat
      301 gaccggcacg ctgtacctgc ctgcgggcta cgacaaagct aaagacggcc cgctgcctac
      361 gctgatgtgg gcctatccgc gcgagttcaa aagcgccgac gcggccgggc aggtgtcggg
      421 ttcgccttat gaatttattc ggctgagctg gggttcgcct ttgtattggc tgacgcaggg
      481 ctacgccgtt ttcgacgatt tcagcatgcc catcatcggc gagggcgatg ccgaacccaa
      541 tgaaaccttt gtggaacaat tgcgctccaa cgccgaggcc gccgtcaata tgttggtcaa
      601 catgggcgtc acagatcgcc ggcgcatagc cgtgggtggg cactcttacg gagcttttat
      661 gacggccaat ctgttggcgc acaccgactt attcgctgcc gggattgctc gcagcggcgc
      721 gtacaatcgc acgctgacgc cctttgggtt tcaatcggaa gagcgtacat tgtggcaggc
      781 gccggaggtg tattccaaca tgtcgccgtt caattttgcc gataagatca aaacgccgat
      841 cctgctcata cacggcgagg ccgacaacaa ttccggcact ttccccatcc aaagcgagcg
      901 cttttatgcc gcgctgaaag ggcatggcgc tacggtgcgc tatgtggtgt tgccggcgga
      961 gagtcatggc taccgggcca aagaatcggt gctgcattgt ttgtgggaaa tggatcagtg
     1021 gttggataag catgtgaaga ataggaaggg gtaagcggct gtaagccgtc atctgctgcg
     1081 cagttcatct gcttgtggcg tctgttacaa agttcataca gagaaacagg ggtgaacggc
     1141 tccaagccgt aagctacacg gttcaaaact cgaatgctat tgtgaaaaca tttctgtctt
     1201 tgttgttggc gatggtgctt tttgtgctgg gcgcctggat ggtatgggaa ttatcnnnnn
     1261 nnnnnnnnng atttaccacc aaaatgcgcg gctgatggag cggttactcg gcttgccggc
     1321 cctgcatctg ggggcgcgga tattgcaaag cctgctgttg ttggctgaaa tttgtgtgct
     1381 cattttgttg gcggcggcgg ccctcattcg ctaccgaaag ccgagggtgt tcatttcttt
     1441 caaatacctg cacgaggaaa aagccaagga aatcggtcgc caggtggagc gcgccggcat
     1501 ggaggtactt cgtcttcctt tcggcagtta cggccacgac gaaattgtgg aatttgtgcg
     1561 cgtggcattg cgcaaagccg atgcggtggt ggtcattccc gatgcggaaa gcgcttcgtt
     1621 tgtggattcc gaattgctgg cggcctcggt gcggcgcatt cccatagcgc tcattcagta
     1681 tcaggagcgg cagtttcagc cgcgcacgct gctgcggggt tatccggtgt tcgactacgc
     1741 ctgtctggag gcgggtgggt ttgaatcgct gaaccggtac ctgtggtttt cggcggggca
     1801 tccgcgagag tatgtgcgca tggccgggcg catttttgcg gtatttctgg agccggtaaa
     1861 ttggatcgtt ttcagtctca tgttggtcgc aatggcgctg atggagggca tcaaatggtt
     1921 gatcaacgcg gcttcttatg ccttgttgca aacggcaatt ttccagccgg acatggaaag
     1981 cgcggtgttt tacaatacgg tttttgtgat gctcctgctc ggctacgctt tgtatttgtt
     2041 gtaccggcag tggaatgtct tgcgtactgc ccggcagata gcctccacag gcacagagag
     2101 ttatcaggtg tttgccgagg cattctccat cctgaaaagc gaccagaaaa tattggcctg
     2161 catccgggag aatttgttca ttccgagggg aaaggggtag gggatgtgtt gtaatttatt
     2221 tagcgaatat atccatttcg ggcctccctg aaaatccact acttgcggag gaatcttcag
     2281 cgaggcccga aataattgct tagaacttcg gagtcaatcc catattgtaa aagcagaaag
     2341 catacatatc ggccactact tccagcgatt ttttcaggtt gctggcgccg tgcccgctct
     2401 ccgtttcgat gcgaatgagt gcggggcggt cggtacgcgc tttggactgc aattcggcgg
     2461 cgtatttgaa ggagtgcgca ggcactacgc ggtcgtcatg atcggcggtg gtaatcagca
     2521 cattggggta gcttgtgccg gctttgatgt tgtgcagcgg cgagtaggca tacaggtttt
     2581 taaaatcctc ctcggtagct tcagcgctgc cgtattctgc aatccagttc cagccgatgg
     2641 tgaatttctg gaagcgcagc atgtccatca cacctacctg aggaatggcc acgccgaaca
     2701 aatcgggccg ctgattgatg accgccccta cgagcagacc gccattggaa cctccctgac
     2761 aggccaaata cttcgtgctg gtgtatttgt tggcaatgag atattccgca gcagcgatga
     2821 aatcgtcgta cacattttgc tttttcaggc gcatgcccgc ttcatgccag gcctcgccgt
     2881 actcgctgcc gcccctgata ttggccgaca cataaatacc gccctgctcc aaccaggnnn
     2941 nnnnnnnnnn atgctgaaac tgggcgtcac gctcacattg aagccgccgt aagcgtacaa
     3001 aatcgtcgga tttttgccgt tcagttccaa tccttttttg tgaatgatga acatgggtac
     3061 cttcgcgccg tctttgctgg tataaaaaac ctgtttggtt tcgtaggcat ccggattgaa
     3121 gtccacttcc ggcgcccgga acagcgtgct cttgcccgta gcaatgtcgt aggtataaat
     3181 agagggcgga taggcaaagc
//
LOCUS       DAORLF010000503         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16622_length_3185_cov_1.837700, whole genome shotgun sequence.
ACCESSION   DAORLF010000503 DAORLF010000000
VERSION     DAORLF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16622_length_3185_cov_1.837700"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PK198_24810"
     CDS             <1..477
                     /locus_tag="PK198_24810"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF42047.1"
                     /translation="LPFALSNMIDSGLLSAQQNMPLDEYLWQYAKQAGKTMYGIETLE
                     DQLGVLGSIDLAHQAHGLLQLARNLPGHRKQLNRLADLYVQGNVSQLYRASRKSLGKY
                     RKTMLFDRNHKMALRFDEIAKTTTLLAAVGAAHLWGGYGLIRLLKAKGWKCKPVIR"
     gene            complement(797..2533)
                     /locus_tag="PK198_24815"
     CDS             complement(797..2533)
                     /locus_tag="PK198_24815"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRF42048.1"
                     /translation="MLMIQNNLDPKVAKHPHELITYGGNGAVFQNWAQYLLTMQYLAE
                     MTEEQTLVMYSGHPMGLFPSSPQAPRVVVTNGMMIPNYSKKEDWNKYNALGVTQYGQM
                     TAGSYMYIGPQGIVHGTTITLLNAGRKLGLGEDSLRGKVYVTSGLGGMSGAQAGAAVI
                     AGCVGVIAEVDGDAVQQRVTDGYILRENVFTDLDQLITRMEDSRRSGTAIALVYHGNI
                     VDLWERFAERGVQVELGSDQTSLHNIDDMGYCPAGYTFEQAKELLIRDKPAFMEAVRD
                     SLRRHVQAVNTMTGRGMYFWDYGNAFLLEAGHAGADVWKNEAEGLYRYPSYVEDIMGP
                     MCFDFGFGPFRWVCTSGLKSDLDLSDRIAAEVIEEMMQEAPDEIMGQLRDNLLWIQSA
                     DENLPVVGSKSRILYADAEGRIRIARAFNQAIAEGRMKIALAFNEAIKKQKIGPVVLG
                     RDHHDVSGTDSPYRETANIYDGSRFTADMAVHNVIGDAFRGATWVSLHNGGGVGWGEV
                     MNGGFGLVLDGSADSERRLQSMLFWDVNNGIARRSWARNEGAMHTAQRAMEMEPNLRI
                     TMPYLVEDEVLE"
     gene            complement(2527..2856)
                     /locus_tag="PK198_24820"
     CDS             complement(2527..2856)
                     /locus_tag="PK198_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42049.1"
                     /translation="MPTNTSFQQAILQGIPAELPPVKPYDATVSHAPRRVIEGVLTAK
                     EKQLALRNALRYFHPRHHAVLAKEFAQELEQYGRIYMHRFRPDYEMYARPSASTRTNA
                     SRRRPSC"
     assembly_gap    2941..2957
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          595 a          969 c          884 g          720 t
ORIGIN      
        1 ttgcccttcg cattatccaa tatgatagac tccggcctgc tgtcggcgca gcaaaacatg
       61 cctttggacg agtacctgtg gcagtatgca aagcaggcgg gcaaaaccat gtacggcatt
      121 gagacgctgg aggatcagtt gggcgtattg ggcagtattg atctggcgca tcaggcacac
      181 gggctgctcc aattggcccg caacctgccc ggccaccgga aacaactgaa tcgcttagcc
      241 gacctgtatg tgcagggcaa tgtgtcgcaa ttgtaccgcg cttcgcgcaa aagcctcggc
      301 aagtaccgaa aaaccatgct gttcgacaga aatcacaaga tggccctgcg ttttgatgaa
      361 attgcgaaga caaccacgct gctcgcagcc gtcggcgcgg cgcatctctg gggcggttac
      421 ggcctcattc gcctgctcaa agccaagggc tggaaatgca aacctgtaat ccgatagctt
      481 gcccgctgat ttcgggttcg aggtttcggg ttcaagcggt cgtttcgcgt aagcgaaagg
      541 taagttcagc gccaataatg atcaaagaag tggcgaatga aggatttttt atagcgatct
      601 gcctctcgca cgcctcgcgg gcaaccgcca acggcggttg aatgtgaata gccccgcgtc
      661 acgaagtgaa cgcggggtat gcgtatcacg ggaagaagaa cccctgacag gggttcaatg
      721 tcattattca cacccgctca tgggacattc aacccctgtc aggggttgcc ctcatcgcga
      781 ttgctacctc acgttcctat tccaacacct catcctccac cagatacggc atggtgatgc
      841 gcaggtttgg ctccatctcc atcgcccgtt gcgcagtgtg catggcgccc tcgttgcgcg
      901 cccaactgcg ccgcgctatg ccgttgttca catcccagaa caacatgctt tgcagacggc
      961 gctccgaatc cgcgctgccg tccaacacca gcccgaatcc gccgttcatc acctcgcccc
     1021 agcccacgcc gccaccgttg tgcaggctca cccaggtagc tccccggaaa gcgtcgccga
     1081 tgacattgtg taccgccata tccgccgtga agcgcgaccc gtcgtagatg ttggctgttt
     1141 cgcggtaggg agagtcggtg ccgctcacgt cgtggtggtc gcggcccagt accacaggtc
     1201 caatcttttg ctttttaatg gcttcattga acgcgagcgc aattttcatt ctgccctcgg
     1261 caatggcctg attgaaggcc cgcgctatac ggatgcggcc ctcggcgtcg gcgtacagga
     1321 tgcgcgattt gctgcccacc accggcaggt tctcatctgc cgattgtatc cacaacaggt
     1381 tgtcgcgcag ttggcccatg atctcgtcgg gcgcttcctg catcatttcc tctatcactt
     1441 cggcggcaat gcggtcgctc aggtccaagt ccgatttcaa acccgatgta cacacccagc
     1501 ggaaaggccc aaacccgaaa tcgaagcaca tcggccccat gatgtcttcc acatacgagg
     1561 ggtagcgata caggccctcg gcttcgtttt tccacacatc ggcgccggca tggcccgcct
     1621 ccagcaggaa ggcattgccg tagtcccaga agtacatgcc gcgcccggtc atggtgttca
     1681 cggcctgcac atggcggcgc agggagtcgc gcacggcctc catgaaggcc ggtttgtcgc
     1741 gtatcaacag ttctttggcc tgctcaaagg tgtagcccgc tgggcagtag cccatgtcgt
     1801 ctatgttgtg cagcgaggtt tggtccgacc ccaattccac ctgtacgccg cgctctgcaa
     1861 agcgttccca caggtccacg atgttgccgt gatacaccaa ggctatggcg gttccgctgc
     1921 ggcggctgtc ctccatgcgg gtaatgagtt ggtccaagtc ggtaaataca ttttcccgca
     1981 ggatgtaccc gtcggtcact cgctgttgca ctgcgtcgcc gtccacttcg gctatcacgc
     2041 ccacacagcc tgctatcacc gccgcgccgg cctgtgcgcc cgacataccg cccagcccgg
     2101 aggtgacata caccttgccg cgcagactgt cttcgcccag tcccaatttg cggcccgcgt
     2161 tcagcagcgt gatggtggtg ccgtgtacga tgccctgcgg cccgatgtac atgtacgagc
     2221 cggcggtcat ttggccgtat tgcgtcacgc ccagcgcatt atatttgttc cagtcctctt
     2281 ttttcgagta gttgggtatc atcatgccgt tggtcaccac cacgcggggc gcctgcggcg
     2341 aggacggaaa caagcccatc ggatgcccgg agtacatcac aagcgtctgt tcctctgtca
     2401 tttcggccag gtattgcatg gtcagcaggt attgcgccca gttctgaaac accgcgccgt
     2461 tgccgccgta ggtgatgagt tcgtgcgggt gcttggccac cttggggtcc aggttgtttt
     2521 gtatcatcag catgatggcc gccgcctgct tgcattggtg cgggtactcg ccgatgggcg
     2581 ggcgtacatc tcgtagtcgg gccggaagcg gtgcatgtat atgcgtccat actgctccaa
     2641 ttcctgcgca aattccttcg ccagcacggc atggtggcgc ggatgaaaat accggagcgc
     2701 gttgcgcagg gccaattgtt tttctttggc cgtcagcaca ccttctatca cgcggcgcgg
     2761 cgcatggctc accgtggcgt cgtagggttt tacggggggc agttcggcgg gtattccctg
     2821 caaaatggct tgttgaaagg atgtattggt tggcattggt tgttaagtag ttgtttgtaa
     2881 aatggtcgtt ggctgtaagc tacaagccgc aggctgcaag cttcaggttg cacgttttca
     2941 nnnnnnnnnn nnnnnnnata tcctatttct ttatggcggc tgcccgctct accgcgagcg
     3001 ctgttggctt tatccctgta atgactcgcc aaagccaaca gctccgatct tatggcgggc
     3061 accgggtcct ctgctcttac cgctgctccg tagcgcggcg gtttcagacc ccgcgctacg
     3121 gagcagcggt accacgccgg cccctgccgc aatcctggcc ctcaacctct cacctccacc
     3181 tcatt
//
LOCUS       DAORLF010000504         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16662_length_3179_cov_1.809219, whole genome shotgun sequence.
ACCESSION   DAORLF010000504 DAORLF010000000
VERSION     DAORLF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_3179_cov_1.809219"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1171
                     /locus_tag="PK198_24825"
     CDS             287..1171
                     /locus_tag="PK198_24825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42050.1"
                     /translation="MTRLIPMAFCSLLFSATLCAQGPIDGYLKGKGNLDIAVSLSNNS
                     AQDFFGKDGAVYNEPFRGRFLALFAEYGFTDDLDIVATLPYVFTSTQSGFQDGSLHLK
                     FRPLRIKTGDGGFFNAVAAAGISAPLSNYEPLAAGAIGQRATVLQPRLVLQWDSGFGP
                     FFNLTGGYNVRLDALREEDIAGVRLLRPDYNPEQPKNFTNWLIKAGLPAANYYLDVWL
                     ERQFTQGGADYAPMVPDLPQAYGVTYTQIGGTAYYSENGKVGFYLSGAHILSGRNTSG
                     ITRITAGVVLKLSTQSTN"
     gene            complement(1156..>3179)
                     /locus_tag="PK198_24830"
     CDS             complement(1156..>3179)
                     /locus_tag="PK198_24830"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42051.1"
                     /translation="PYHVTAENDAHKDKVSVYTRKAPFQFGWDWGPRMVSCGIWRPVY
                     LLSWQDARVEDVQIFQEQLSDERADLRAAFRLSGAAPAGAQWVLRDAVTGAEYGRTAA
                     GDTAAELKFNIRNPRRWWTHNLGEPYLYRFEALLMQGDAVLDRREIRFGLRTIEVIQE
                     EDSIGRAFYFKLNGVPVFMKGANYIPGDALLPRRTPDDFRRLLQSAKDVNMNMIRVWG
                     GGIYEEDLFYDLCDEMGLLVWQDLMFACSMYPGDDPEFFENVRREVTYNARRLRHHPS
                     IAIWCGNNEIDMAWHHWGWQVRYLIAPGKAAKMWDAYQALFHRLFPAIIQNEDPGRTY
                     VSSSPQSNWGKAENFNFGTMHYWGVWHGPDDFEGYKRNVGRYMNEYGFQSFPEWTTIR
                     AFSQPTDWSLESEVMKRHQKSYIGNGVIAKFTNKHYKTPTDFQDFVYKSQLTQADGMR
                     AAISSHRARKGHCMGTMYWQLNDCWPGPSWSGIDYFGRWKALHYTLRELYADLLIVAD
                     NDQSRLNVNLVSDQLKPVKGNLLIEVFRLDGRRVSSLMFDLDVPANSAGQYLALPLKT
                     LLKHAKPQQVFARIRFMDASGELASTMHYFAPAKLMPLEKPELDIAVDEDTRTVTVSS
                     KTLVRSLFLYSASGDCRFSDNFFDLLPGEARTVRYEGALGDGIKFQFVD"
BASE COUNT          674 a          844 c          915 g          746 t
ORIGIN      
        1 cggagccttg ttcgtcagac tcgctgctga ccaatgaaag aatgctgagg tgaacgactg
       61 ccatgataaa tcctgctgtt tgcggtcgag ttgaaaaata ggcgtttttt tgtcgccggt
      121 ttcgggcctc aagatagggc agtttttata tttttgtcaa aatcgttgaa aagaaactcc
      181 atacgctact caatcgtcct tttcggctgt ctccattgcg acaagaagag cagttttttt
      241 gaatagtatt actattgtca aattactttt gcccaaaatc tgaacaatga cacgtttgat
      301 tccaatggcg ttctgctctc tccttttttc tgccaccctt tgcgcacaag gccccattga
      361 cggctatctg aaaggcaaag gcaatcttga cattgccgtt tctttgagca acaactccgc
      421 tcaggacttc ttcggaaagg acggggccgt ttacaacgag ccgttccggg gccgattttt
      481 agccttattt gcagaatatg ggtttaccga cgatttggac atcgttgcca ccctgcccta
      541 tgtcttcact tctacgcaga gcggttttca ggatggcagt ttgcacctga aattcaggcc
      601 cttgcgcatc aaaaccggcg atggaggttt tttcaatgcc gtggctgcgg ccggtatcag
      661 cgcgcctttg tcgaactacg aacccctggc tgccggggct atcggccaac gcgctacggt
      721 gctgcaacct cgtttggtat tgcaatggga ttcggggttc gggccgtttt tcaacctgac
      781 cggaggctac aatgtgcgat tggatgccct gcgcgaggaa gacatcgccg gcgtacggct
      841 cctgcggccg gattacaacc ccgaacagcc caaaaatttc accaactggc tcatcaaagc
      901 gggattaccg gcagccaatt actatctgga tgtctggctc gaacggcagt tcacgcaggg
      961 cggcgcagat tacgctccaa tggtacccga tctccctcag gcttacggcg tcacttatac
     1021 ccaaatcggc ggaacggctt attattccga aaatggaaaa gtgggttttt acctcagcgg
     1081 ggcgcacatc ctcagcggca gaaacaccag cggcatcacg cgcattacgg cgggcgtagt
     1141 gctgaagtta agtactcaat ccacaaactg aaattttatc ccatcgccca atgcgccttc
     1201 gtatcgtacc gtgcgcgctt cgcccggcag caagtcgaag aaattgtcgg agaaacggca
     1261 atcgccagac gccgagtaca ggaaaaggct gcgaactaat gttttagacg acacggtgac
     1321 ggtgcgggta tcctcatcta cggcaatatc aagttcgggt ttttcgagcg gcatcagctt
     1381 agccggcgcg aaatagtgca tggtcgaagc cagttctccg gaggcgtcca tgaagcggat
     1441 gcgggcaaaa acctgctgcg gtttggcgtg cttgaggagg gttttgagcg gcagcgcgag
     1501 gtattggccg gcggaattgg ccggcacatc cagatcgaac atgaggctgg aaacccggcg
     1561 cccgtccaag cgaaatactt cgatgaggag attgcctttc acgggtttga gctgatctga
     1621 aactaagttg acgttcaggc ggctttggtc gttgtcggcc acgatgagca gatcggcgta
     1681 caactcgcgg agggtgtagt gcaatgcttt ccaacgcccg aaatagtcta tgcccgacca
     1741 ggaaggcccc ggccagcagt cgttgagttg ccagtacatg gtgcccatgc agtgcccttt
     1801 gcgggcgcgg tggctgctga tggcggcacg cataccgtcg gcctgagtga gttgactttt
     1861 gtacacaaaa tcctgaaaat cagtaggcgt tttgtagtgt ttgttggtaa acttggcaat
     1921 caccccattg ccgatatagc ttttctggtg gcgtttcatc acctccgatt cgaggctcca
     1981 gtcggtgggt tgcgaaaagg cacggatggt cgtccattcc gggaaggact gaaatccgta
     2041 ctcgttcata tagcggccca cgttgcgttt gtagccctca aagtcgtcgg ggccgtgcca
     2101 cacgccccag tagtgcatgg tgccgaagtt gaagttttcg gctttgcccc agttgctttg
     2161 cggcgaagag ctgacgtaag tgcggccggg gtcttcgttt tggatgatgg ccgggaaaag
     2221 ccggtggaag agcgcttgat aggcatccca cattttggcg gccttgcccg gcgcaatgag
     2281 gtagcgcacc tgccagcccc agtgatgcca ggccatgtca atttcgttgt tgccgcacca
     2341 gatggctatg ctgggatggt ggcgaaggcg gcgggcgttg taggtcactt cgcggcgcac
     2401 gttttcaaaa aattccgggt cgtcgccggg gtacatgctg cacgcgaaca tgaggtcttg
     2461 ccacaccaaa agccccattt catcacagag atcgtaaaac aggtcttctt cgtaaatacc
     2521 gccgccccaa acgcgtatca tgttcatatt cacgtctttg gccgattgta gcagtcgcct
     2581 gaagtcatcg ggcgtgcggc ggggcaggag ggcatcgccg ggaatgtaat tggcgccttt
     2641 cataaacacc ggcacgccgt tgagtttgaa ataaaacgcc cgcccgatgc tgtcttcctc
     2701 ctgtatgacc tcgatggtac gcaggccgaa ccggatttcg cggcggtcga ggacggcatc
     2761 gccctgcata agcaaagcct cgaatcggta aaggtacggt tcgccgaggt tgtgcgtcca
     2821 ccagcggcgg ggattgcgga tgttgaattt caattcggca gcggtatcac cggcggctgt
     2881 gcggccatat tctgctcctg taacggcatc gcgcagcacc cactgagcgc ctgcgggcgc
     2941 ggcgccggag agccggaaag cagcgcggag atcggcgcgt tcgtcgctga gttgttcctg
     3001 aaaaatctgc acatcctcta cgcgcgcatc ttgccaggac agcaggtaaa ccggccgcca
     3061 gatgccgcaa ctgaccatgc gcggccccca gtcccagccg aattggaagg gcgctttgcg
     3121 ggtgtaaacg ctgactttgt ctttgtgggc gtcgttttct gcggtgacgt ggtagggca
//
LOCUS       DAORLF010000505         3170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16730_length_3170_cov_2.209310, whole genome shotgun sequence.
ACCESSION   DAORLF010000505 DAORLF010000000
VERSION     DAORLF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16730_length_3170_cov_2.209310"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK198_24835"
     CDS             <1..351
                     /locus_tag="PK198_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF42052.1"
                     /translation="YFQLTPIRKPTLERKVNQLKEAGVVLLIGTDSGIPMKFHCQSTW
                     NELDVWVNEFGFDPMYAIKAATYWPAKSMGVDKDYGTISEGKYADIIAVKGDVLRYIS
                     LLQDVKMVMKHGRR"
     gene            563..650
                     /locus_tag="PK198_24840"
     tRNA            563..650
                     /locus_tag="PK198_24840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:597..599,aa:Ser,seq:tga)
     gene            complement(790..>2595)
                     /locus_tag="PK198_24845"
     CDS             complement(790..>2595)
                     /locus_tag="PK198_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42053.1"
                     /translation="SLHYSPAGQLWAGTDYGVSSLNLENNLTYFYDYRGDFGTGYTAL
                     LKDDIFYLGTNQGLYRSRWEDLNNNSDFIRFQLVEGTEGQVWSLRETGAGVLIGHDRG
                     LMSLDGNSPRFLGKQGGVWTMLPYRDYLLTGNYNGISIFKKSENGWAFWKKMELILGS
                     CNQLLIQARDVLWVNIPNYGIIRAQLDDHLSPTDRAIFYENQFEGIRPHLLKKGNDIE
                     VVTDKYRYLYDEKKRQFNRTDIPAPPLEVEALLPGVYEPFALRADYDFYPVYNGFALH
                     RREKNERNRPIQPTLVLRKIEAFNNDHRTAFYSGAKVPYRLHNLSIECLIPHQKQVLY
                     QYKLNDEEWSQESPQHTFQFFGLSEGAYTLSVRAVINGALTAPYHIDFQIQTPWYRSW
                     YAYFFYVLALAGLILAFRKWQRVSLLKQKKQLLLKAQSSLREQAEKHRHAIMLMEQEQ
                     LQKEYEQLKLQLKNKTVELANKAKDSEDKNRLLLILKEKLEDAQANPSKSKIRWNEMQ
                     SLLDSYLATEDKVFEIQMDELHQEFFKKLKGQFPGLSNQDLRLCAYLKIGLNTKEIAD
                     ILNILPSSAFITRSRLRKKLNLNEDQDLHTFLNSL"
     assembly_gap    2596..2615
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2616..>3170)
                     /locus_tag="PK198_24850"
     CDS             complement(<2616..>3170)
                     /locus_tag="PK198_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42054.1"
                     /translation="QAFEYTSLYPLREAAGEVNEEFWDLHRRQGEVLFVSFQNIYIYK
                     DSKLRTIAAPHRFAGSFKVGDALFLADEKEGLFRLDGASLQLVFKQPDDLKFEISGIY
                     ENDEGLVVVTKNAGLYLWAGGKLRAMDTPFSRILKTARAFSFELLEDQHLAFGTVLKG
                     LYITDLNGRILHHINKYKGLPNNT"
BASE COUNT          745 a          718 c          842 g          845 t
ORIGIN      
        1 tatttccaac tcacgcccat ccgcaagcct acattggagc gcaaggtgaa ccaactgaag
       61 gaggcgggcg tggtgctgct gataggtacg gacagcggta ttccgatgaa gtttcactgc
      121 cagagcacct ggaacgagct ggatgtgtgg gtgaatgaat ttggtttcga cccgatgtat
      181 gccatcaaag cggcgacata ctggccggcc aagtctatgg gtgtggacaa ggactacggc
      241 accatatcgg agggcaaata cgcggatatt attgcggtga agggggatgt gttgcgctat
      301 atcagtttgt tgcaggatgt gaaaatggtg atgaagcacg gtaggcggta ggcggtaggc
      361 ggtggccgcg ccgtggcgcg gtaagcgtga ggtggtccgg tatgcggtat gaggtgggca
      421 gtgggcggtg taaagcgtgg ggtggtccgg taggcggtgg gtgtttgtgc cttgcaaggt
      481 cttttttatc tgtggggaat ttattttttt caaaatggct ttgtagtttc gttgcgaagc
      541 gtatttttgc agccgcaaat ccggaggggt ggcagagcgg tttaatgcgg cggtcttgaa
      601 aaccgttgac tgtaacaggt ccgggggttc gaatccctcc ccctccgcca aagccgaaag
      661 ggccgtcgtt gagacggcct ttttcatttg gcgcaaccgc gaccgaatgc aattcgaggc
      721 gctgttgtga cgaatgaaaa agcggacggg cgaagaccgg gcggcctttc gggtttgaag
      781 ccccccattt cacagcgaat ttaaaaacgt gtgcaaatcc tgatcttcgt tcaggttgag
      841 ctttttgcgc aggcgcgagc gcgtgatgaa agcactggaa ggcaggatgt tcagtatgtc
      901 ggctatttct ttggtattca gaccgatttt cagataagcg cacaatctta agtcttgatt
      961 ggacaacccg ggaaattggc ctttcaattt tttgaaaaac tcctgatgta attcgtccat
     1021 ctgaatttca aaaaccttgt cttctgtggc taaataggag tctaataaag actgcatttc
     1081 attccacctg atttttgatt tggaggggtt ggcctgtgcg tcttccaact tttctttgag
     1141 gatgagcaga aggcgatttt tgtcttcact gtctttggcc ttattggcta attctaccgt
     1201 cttgtttttc aattgcaatt tgagctgttc gtactctttt tgcaactgtt cctgctccat
     1261 caacatgatg gcgtgcctgt gtttttctgc ctgttcgcgc agagaggact gcgcttttag
     1321 caacaactgc tttttttgtt tgagcaacga taccctctgc cacttccgaa aggcgaggat
     1381 aagtccggct aatgccaata cataaaaaaa ataggcatac caactgcgat accaaggcgt
     1441 ttgtatctga aaatcaatat gataaggcgc ggtgagcgct ccatttatca ccgccctgac
     1501 ggagagtgta tatgcgcctt cgctcaatcc gaaaaactga aaagtatgct gcggcgactc
     1561 ctggctccac tcttcgtcgt tcagtttgta ctggtacagc acttgttttt gatgggggat
     1621 caggcactcg atgctcaggt tgtgcaggcg atagggtact ttggcgccgg agtaaaaagc
     1681 agtccggtga tcgttgttga aagcctctat ttttctgagt accagcgttg gttggattgg
     1741 tcggtttctc tcgttttttt cgcggcgatg gagggcaaaa ccattgtaaa ccgggtaaaa
     1801 atcataatcg gcgcgtaaag caaacggctc ataaacaccg ggcaataagg cttccacctc
     1861 aagcggggga gcagggatat ctgtacggtt gaattgtcgc ttcttctcat cataaagata
     1921 ccggtattta tctgtaacta cctcaatatc attgcccttc ttgagtaaat gaggcctgat
     1981 tccctcaaat tgattttcat aaaaaatagc ccggtcggtg ggcgataaat ggtcgtccaa
     2041 ttgcgcccga atgattccgt aattgggaat gttcacccac aaaacgtcgc gggcttgaat
     2101 cagaagctga ttgcaggagc caagaatgag ttccattttt ttccaaaaag cccagccgtt
     2161 ttcgcttttt ttaaaaatgg aaatgccatt gtaattgccc gtcagcagat aatcccggta
     2221 gggcagcatg gtccaaacgc cgccctgttt gccgaggaat cggggcgaat ttccatccaa
     2281 agacatcaga ccccgatcat gcccgatgag cacgccggcg cccgtttctc tcagcgacca
     2341 aacctgcccc tctgtgcctt caacaagctg aaacctgata aaatccgagt tattattgag
     2401 gtcttcccaa cgcgaccgat acaagccctg attggtaccg aggtaaaaaa tgtcgtcttt
     2461 caacagggcg gtatagccgg tgccgaaatc gccgcgatag tcgtagaaat aggtgaggtt
     2521 gttctccaaa ttcagtgagg aaacgccata atcggtgcct gcccagagtt gcccggcggg
     2581 gctgtaatgc aggctnnnnn nnnnnnnnnn nnnnngggta ttattgggca agcccttgta
     2641 tttattgata tggtgcagga ttcttccgtt cagatcggtg atgtataagc ctttcagcac
     2701 cgttccgaat gccagatgtt gatcttcaag caactcgaaa ctgaatgccc gggctgtttt
     2761 taaaatccgg gaaaacgggg tatccatagc gcgaagtttg ccgcctgccc agaggtacaa
     2821 acctgcattt ttggtgacga cgaccaagcc ctcatcgttt tcataaatgc ctgatatttc
     2881 aaactttagg tcgtccggtt gtttgaatac caattggagc gaggccccgt cgaggcggaa
     2941 cagtccttct ttttcatcgg ccagaaacag cgcgtcaccc accttgaaac tgccggcaaa
     3001 acggtggggc gcggcaatag ttcggagttt tgaatctttg taaatataga tattctgaaa
     3061 tgatacaaac agtacttcgc cctgccggcg gtgcagatcc cagaactctt cattcacttc
     3121 gccggcggct tcccggagcg ggtataagga cgtatattca aatgcctgat
//
LOCUS       DAORLF010000506         3169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16738_length_3169_cov_2.089274, whole genome shotgun sequence.
ACCESSION   DAORLF010000506 DAORLF010000000
VERSION     DAORLF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16738_length_3169_cov_2.089274"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1020
                     /locus_tag="PK198_24855"
     CDS             133..>1020
                     /locus_tag="PK198_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF42055.1"
                     /translation="MRILLFCTAFFVAFTAGAQTILDTLQLSHSPADTTTAKTQLAKA
                     DDLLRLRKYKEAKEHAVAARQIAAAALGSDNVYVVRALVAEARALIGLRDFDEAMNVL
                     QHAETLCSKLPEDTRTDQAYIALNIGLVWDARQEPERAIEPKLKALDWYLQTLPRNDR
                     RIAVCANSLGNSYESNGNADKALEYKTLALEIIELISGPNSLDAAVLHNNLGIAHWGA
                     GNLEQALYHHSKALAIRQKVLPAGEYLIGESHNNLALVYESQGQLSKARDAYSAATSI
                     FEGKEDQLVFGRLAKNVYNN"
     assembly_gap    1021..1041
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1042..>2792
                     /locus_tag="PK198_24860"
     CDS             <1042..>2792
                     /locus_tag="PK198_24860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF42056.1"
                     /translation="MGLTEQAVSAKRRSLDLSIRLYKEQSIETAQAWTNLGSALRDAE
                     DFDAALSCFQKSLEIRAALGQPNHAHNTFTLVNKGAVHIEFGQIDTALANFQQALDLA
                     FLHRPDDPAEWALCYSYLGQGYAAAKNWQVSADYYHQCRAIRQNKYASSHPGIILALL
                     GIARAHHALAQPDSAQYYFQLAFDLQNPSGDTGYIGDPHNWLSALSGLATLNSERFIN
                     SQMPVYLNVVNELAEQCISFLERQRIQNPYRQDHFRLSAAAFPVFEAAVQANYFAHQQ
                     SGHTDDAGRCFRYSEIIKGQQLYEAFKAARAEKFADIPPILLEAEDSLAVELAYLENQ
                     LLEKKNAARQETDSVILLLHKRIVTTQEAYLQHRALLRKLVPEYYRIKYELSPMDLGR
                     LQKSLPAGDSAAMLEYFVGDNIVLAFVIRPDTVFIQQLSLPLSWEEQVADFRTGLFGA
                     FIPNRPDSLPLHTNRLFAQSAVVLYEMLVAPIQKHLPERLIIVPDGIMGFIPFEALVT
                     EPITGRTDRFQEHKYWGRDKVISYAYSATLWQEMKTKQHLQNPAKTMLAMAPFFRGDW
                     KALEKMLDKEPADADIA"
     assembly_gap    2793..2855
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2856..>3169
                     /locus_tag="PK198_24865"
     CDS             <2856..>3169
                     /locus_tag="PK198_24865"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF42057.1"
                     /translation="IARRYKGLALLDVMATRAAFDTMAASYRRIHLSTHGYAGDDSWL
                     AFYKSGRPLDADKLYLRDIYNLRLNADLVVLSACQTGIGKLRRGEGIISLSRAFAYAG
                     AK"
BASE COUNT          776 a          878 c          735 g          696 t
ORIGIN      
        1 tagataagac acttaaaatg aaacgtttac ttatctaatc ctttgacgaa aatgatcact
       61 ttattttttt atcgcccctt acaatattca ccggctgcat ttattttcat caaaacatat
      121 acctgcctac ccatgcgaat actccttttc tgtactgctt tttttgtggc atttaccgcc
      181 ggcgctcaaa ccatattgga tacgctccaa ctcagccatt cgcccgcaga caccacgaca
      241 gccaaaacgc aattggcaaa agccgatgac ttgctgcgac ttcgaaaata taaggaggcc
      301 aaagaacacg ctgtggccgc ccgacaaatc gctgccgctg ccctcggctc cgacaatgtg
      361 tatgtcgtgc gcgccttggt ggcggaggcg cgcgcgctca ttggtttgcg ggactttgat
      421 gaggccatga acgtgttgca acatgcggaa accttgtgtt ctaagcttcc ggaagatacg
      481 cgcaccgatc aggcttatat cgctctcaac atcggtctgg tatgggatgc ccggcaagaa
      541 ccggaaagag ccatagaacc aaaactcaaa gcactcgact ggtatctgca aacactcccc
      601 cgcaacgacc gccgcatagc cgtttgtgcc aacagcctcg gcaacagcta cgaatcgaac
      661 ggcaatgcag acaaagcgct ggagtacaaa accctggcat tagagattat agaactcata
      721 tccggcccca acagcctgga tgctgccgtt ttgcataaca acctcggcat agcgcattgg
      781 ggcgccggca atttggaaca ggcactttat catcactcca aagctttggc cataaggcaa
      841 aaagtactgc ccgccggcga atacctcatc ggcgaatcgc acaacaatct ggccttggtg
      901 tatgaatcgc aggggcaatt gtcgaaagcc agagacgcgt atagtgctgc cacgtccatt
      961 tttgaaggca aagaagacca actagtcttt ggtcggctgg cgaaaaatgt ttacaacaat
     1021 nnnnnnnnnn nnnnnnnnnn ncatggggct gaccgaacag gccgtttcgg caaaacgacg
     1081 gtcactcgac ctgtccatcc gtctttataa ggaacaaagc atagagaccg cccaagcctg
     1141 gaccaacctc ggttcggccc ttcgcgatgc agaggatttt gacgccgcgc tgagctgttt
     1201 ccaaaaatcc ttagaaatca gagccgcttt ggggcagccc aaccatgctc acaatacctt
     1261 taccctggtc aacaaaggcg ccgtacacat cgaatttggc cagatagata ctgccttagc
     1321 caatttccaa caggctcttg acctcgcttt tctccatcgc ccggatgacc ccgcagaatg
     1381 ggccttgtgt tacagttatc tggggcaagg ctatgcggcc gcaaaaaact ggcaggtctc
     1441 tgccgattac taccaccaat gccgcgctat caggcaaaac aaatatgcct cctcgcatcc
     1501 gggcatcatt ttggcgctgt tgggcatagc ccgggcgcat catgccctgg cgcagccgga
     1561 tagtgcgcaa tactatttcc aacttgcttt tgacttgcag aatccctccg gcgatacagg
     1621 gtacataggc gaccctcata attggctcag cgccttatca ggtttggcaa ccctgaacag
     1681 cgagcgcttt atcaacagtc aaatgcctgt ttatctgaat gttgtcaatg aattggccga
     1741 gcagtgcatt tcctttctgg agcgccaacg catacagaat ccatatcgtc aggatcattt
     1801 cagattgtct gccgctgcat ttcccgtatt tgaagcggcg gtgcaggcca actatttcgc
     1861 acaccagcaa agcggccata ccgacgatgc cgggcgatgc ttcaggtaca gtgaaatcat
     1921 caaagggcaa caactgtatg aggcgtttaa agctgcccgc gccgaaaaat ttgcagacat
     1981 accgccgatc ctgctcgaag cggaagactc gctggcggtg gaattggcgt atttggagaa
     2041 tcaactattg gaaaaaaaga acgccgcaag gcaggaaaca gattcggtca tcctgctgct
     2101 acataaacgc attgtaacaa cacaggaggc ctaccttcaa cacagagcat tgctccgcaa
     2161 gttggtcccg gagtattacc ggattaaata tgagctatcc ccgatggact taggccgcct
     2221 gcaaaaatcg cttcctgccg gtgatagcgc tgccatgctc gaatattttg tgggcgacaa
     2281 catcgtgttg gccttcgtga tacgccccga taccgtattc atacaacagc tctccctgcc
     2341 gctttcctgg gaggagcagg tagccgattt tcgtaccggg ttgtttggcg catttatccc
     2401 caaccgaccg gactcgctcc ctcttcacac caaccggctt tttgcccaaa gcgccgtcgt
     2461 tttatatgag atgttggtgg cccccatcca aaagcacctg cccgaacgct taatcatcgt
     2521 acccgacggc atcatggggt tcatcccttt tgaggcattg gtaacggaac cgataacagg
     2581 cagaaccgac cgatttcagg agcataaata ttggggccgg gataaagtaa tttcttatgc
     2641 ttattccgcc accctctggc aggaaatgaa gacaaaacag cacttgcaaa atcctgcaaa
     2701 aacgatgctc gccatggctc cgtttttcag aggagactgg aaagccctcg aaaaaatgct
     2761 ggataaagaa cccgctgatg ccgatatagc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcatt gcacggcgct acaaagggct
     2881 tgctctgttg gacgtcatgg ccacccgcgc ggcatttgat acgatggccg cttcgtatcg
     2941 ccgcatacac ctcagcacac acggctacgc cggagatgat tcctggctcg ccttttacaa
     3001 atccggccgg ccacttgacg ccgacaaact ctaccttcgc gacatctaca acctccgcct
     3061 caatgccgac ctcgtggtgc tctccgcctg tcagaccggc atcggcaagc tgcgccgcgg
     3121 cgagggcatc atttctctgt ccagagcatt tgcgtatgcc ggggccaag
//
LOCUS       DAORLF010000507         3159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16797_length_3159_cov_2.026418, whole genome shotgun sequence.
ACCESSION   DAORLF010000507 DAORLF010000000
VERSION     DAORLF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16797_length_3159_cov_2.026418"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1000)
                     /locus_tag="PK198_24870"
     CDS             complement(173..1000)
                     /locus_tag="PK198_24870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42058.1"
                     /translation="MAAVVEGCNGINSTGLVRIFPLENPAPSIASDCRENTAAYDPNA
                     KSASVIGLDEERFIRSGTDLEYLIRFQNTGTDTAFRVVILDTLSQWLRPESVRPGATG
                     HPYSFELLEGGILRFSFDNIMLPDSNVNEPASHGFIQFWVRQQADTPPGTRIENTAAI
                     YFDQQTPVITNTVFHTIGDHLIQAASNDEEAQEIWGALTVFPNPSAGDADFLLPASLS
                     DANVFFELYDVWSRPVQQGSFQNGRYGFRKGDLSSGVYFYRIKTAGRVAYSGKIVIK"
     gene            complement(988..1164)
                     /locus_tag="PK198_24875"
     CDS             complement(988..1164)
                     /locus_tag="PK198_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42059.1"
                     /translation="MNEPMEFIVVEDVIMYLRDQFQLESGQTLNLPAIPSNGQTWRLE
                     APQALTPIPARWLR"
     gene            1210..2622
                     /locus_tag="PK198_24880"
     CDS             1210..2622
                     /locus_tag="PK198_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42060.1"
                     /translation="MAKAFHFNVGARPISFSLIEQREVRRFLLSPFFNTRSDLLQLFD
                     LYCAEPLAEKPQVWSALFPEQTFDDQKLRLLMSYLFKLLEQYLIIKTATSDPAQNLLH
                     LAAGYRQRAMTHAFERTRTSLTQVLTSRPQRDAQYFERLYRLHWESYQTAAAQNPSEA
                     LPLRELAYAADVHYFSTRLRLICLAAAQQGVYRADYHSSEDEAILVRAGQEPWASVTA
                     VAVYLHCWKMIQHPEEEFHFQRFKEILLCRSEEFDHEEIRGLYLLAINYCIKRVNEGH
                     KIFFGEVLDLYKSGLEGGRLFENGLLSRFTYHNIVAAGLQLKDYEWVAHFIHEYKSSL
                     ERAYRESSFSFNLARLEYSRQNYPMVLQLLQGANYRDTLLNLAAKTLLMKTWYELGET
                     EVLFAHIDAMRNYIRRKKVIGYHKTNYLNVLKYTDKLLKINRFDAAAVALLKAEIEAE
                     EILTERTWLIEQLQILTART"
     gene            2741..3076
                     /locus_tag="PK198_24885"
     CDS             2741..3076
                     /locus_tag="PK198_24885"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF42061.1"
                     /translation="MRFSAQISQDTAALHRLLADDLVYIHSNALVETKTDFIRSVGGG
                     GIRYLAMSSTSPAEIRQWGKTAVAVGIVSVRGLYQGTEFDMRLRYTSVYRKNKGIWQL
                     SSWQSTRLP"
BASE COUNT          780 a          807 c          796 g          776 t
ORIGIN      
        1 gagtcacgag ctatgagttt gtttattcga tgaaaaatta gattttccat ttgggaaaat
       61 ttcaaaatct cgtcttgaaa aaagaccatt tttcattttg ggaaatcaaa ctcgtgactc
      121 gaagctcgcg gctcgcagct ttttgcaggg cgactgtata gtaacctgca atttatttga
      181 tgactatctt gccgctgtat gcaacgcggc ctgctgtttt aatccggtag aaataaactc
      241 cggaactcag atcgcctttt ctgaaaccat atcgtccatt ctggaaacta ccttgttgaa
      301 caggtctgct ccaaacatcg tacagctcaa aaaacacatt tgcatcgctc agcgacgccg
      361 gcaacagaaa gtccgcgtct ccggcagagg gattgggaaa tacggtgagc gctccccaga
      421 tttcctgcgc ttcctcatca ttgcttgctg cctgtatgag atggtcgccg atggtatgga
      481 acaccgtatt ggtaatgaca ggcgtctgtt ggtcgaaata gatggctgcc gtgttttcaa
      541 tgcgggttcc cggcggggta tctgcttgct gccgcaccca aaactgaatg aatccatgcg
      601 atgcgggttc gttgacattg ctgtcaggca gcatgatgtt gtcaaaacta aatctcagaa
      661 tgcctccctc caacagttca aaggagtaag gatgtccggt agctccggga cgaacgcttt
      721 caggccgaag ccattgcgaa agcgtatcga gaatgactac gcggaaagcg gtatcggtac
      781 cggtgttctg aaagcgaatg aggtactcca gatcggttcc tgatcgaatg aaacgttcct
      841 catccaaacc aattacgctt gcgctcttgg cgttcgggtc gtacgcagcg gtattttctc
      901 gacagtcgga agctatagac ggggcggggt tttctaaagg gaagatgcgc actaagcccg
      961 tggagttgat gccgttgcag ccctctacta ccgcagccac cgtgccggaa taggggtgag
     1021 cgcttggggc gcctccagac gccaggtttg accattggag ggaatagcgg gcaagttcag
     1081 cgtttggccg ctttcgagtt gaaattgatc gcgcagatac atgataacat cctctaccac
     1141 aatgaattcc atcggttcgt tcatgccgcc tgagccggtg tttttgatgc gccaatatac
     1201 agagtcgttt tggcaaaagc cttccacttc aatgttggcg cccgaccaat ctcattttcg
     1261 ctcatagaac agcgggaagt gcggcgtttt ctgctgtcgc cgttctttaa tacccgaagc
     1321 gatctgttgc agttgtttga tctgtattgt gcagaaccgc tcgccgaaaa gccgcaagtg
     1381 tggagcgcct tgtttcccga acaaactttt gacgatcaaa aactgaggct tctgatgagc
     1441 tacctcttta aattattgga gcaatatctc atcatcaaaa cagctacctc cgaccccgcc
     1501 caaaacctgc tccatctcgc cgccggatac agacagcggg ctatgacgca tgcttttgag
     1561 cgcacgcgga cctcgttgac ccaagtcctg acgagccgac cccagcgaga tgcccaatac
     1621 ttcgagcgcc tttaccgcct gcattgggaa agctaccaga ctgcggcggc gcaaaacccc
     1681 agcgaagccc tgcccctgcg cgaattggcc tacgcagccg acgtccacta tttcagtacc
     1741 cgactgcgcc tcatttgtct ggccgctgct cagcagggcg tttaccgcgc cgactatcac
     1801 tcctccgaag atgaggctat tttggtacgg gccgggcagg agccgtgggc gtcggtaacg
     1861 gctgttgcgg tgtatctgca ttgctggaag atgattcagc acccggagga agagtttcat
     1921 tttcaaaggt ttaaagagat attactctgc cgttcagaag aatttgatca cgaagaaata
     1981 agaggattgt acctgctggc catcaactat tgtatcaaaa gagtgaatga ggggcacaaa
     2041 atattttttg gcgaagtgct ggatttgtac aaatccggtc tggagggcgg aaggcttttt
     2101 gaaaacggcc tgctctcccg ctttacttat cacaacattg tggcagccgg actgcaactc
     2161 aaagattatg aatgggtagc gcactttatc cacgagtata aaagtagcct tgaacgcgct
     2221 tatcgggaaa gctccttcag ttttaacctg gcaaggttgg aatactcccg tcagaactac
     2281 ccgatggtgc ttcaactgtt acagggcgcc aattatcgcg atactttgct gaacctggca
     2341 gcgaaaaccc tgttgatgaa aacctggtac gagctgggcg aaacggaggt actctttgcc
     2401 cacatagacg ccatgcgcaa ttacatccgg cgcaaaaaag ttattggata ccacaaaacc
     2461 aattatctga atgttttgaa atatacagat aagttattga aaataaaccg attcgatgct
     2521 gctgctgtcg ccctgctcaa agccgagata gaagcggagg agatattaac tgaaagaaca
     2581 tggctcatcg aacagttgca gatattgacc gctcgaacct gatcatcaac acgctatcat
     2641 catgaaaatc atccattttt ttcaactcct cgccgtctgt gggctattgg cgtgcccagg
     2701 cctttcgtct gctcaaaaaa cgattgttct atcagagcaa atgaggtttt cggcacaaat
     2761 cagtcaagac accgccgccc tgcatcgcct gctcgccgac gacttggtct atatccattc
     2821 caatgccttg gtggagacca aaaccgactt cattcgaagc gtgggaggcg gcggcattcg
     2881 ctacctcgcc atgagcagta cctcgcccgc tgaaatcaga cagtggggga aaaccgccgt
     2941 agcggtgggc attgtgagcg tcagagggct gtatcagggc acagaattcg acatgcggct
     3001 gcgctacacc tcggtttatc gcaaaaataa aggcatctgg caactcagct cttggcagtc
     3061 cacgcgcctg ccttgatggt tcctttacgg cttagttgtg tctcgcgggc aaccgccaac
     3121 ggcggttgaa tacgaatagc cccgcttatt caagcggca
//
LOCUS       DAORLF010000508         3149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16869_length_3149_cov_2.357143, whole genome shotgun sequence.
ACCESSION   DAORLF010000508 DAORLF010000000
VERSION     DAORLF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16869_length_3149_cov_2.357143"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK198_24890"
     CDS             <1..643
                     /locus_tag="PK198_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42062.1"
                     /translation="VVMRESSAAGAKKAQLLTNLGQYHRREFRTATNGAATPQQISSN
                     GRHWLRIVRVGNQFTLYASGNGTSWFIIGAQNIVMSNCIELGLVATNQTANSAVTATF
                     ANMSFSGSNSTLGTNSIEQRAESRDMPHSFEVYPNPTGGELNVDLTQYVGRDVRIEVY
                     SIEGKLLQFREIEEVQTTMENLNLSQYAGGMYFVKIKSHDLPDATRRVVLQRE"
     gene            1008..>1599
                     /locus_tag="PK198_24895"
     CDS             1008..>1599
                     /locus_tag="PK198_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF42063.1"
                     /translation="MLKQNNPITMFTPIVQPARRLAGINEYYFSAKLREIAQMRAAGK
                     DVINLGIGSPDLPPAAETVEALVKHSRERHTHSYQSYTGIPELRQAFAHWYGQWFGVP
                     LNPDNEVLPLIGSKEGIVHIAMTYLSEGDQALVPNPGYPTYRAATILSGAEPLTYELL
                     PEQGWLPDLEALEQQDLSRVKIMWINYPNMPTGAAAP"
     assembly_gap    1600..1609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1610..2230
                     /locus_tag="PK198_24900"
     CDS             <1610..2230
                     /locus_tag="PK198_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF42064.1"
                     /translation="FFEKLIAFARRNRILLVNDNPYSFILNDRPASILAVEGAKDVAI
                     ELNSLSKSNNMAGWRVGVLAGRFDYLQEVLRFKSNMDSGMFKPLQLAAAQALASPPEW
                     YAQLNTVYRERRENAYRLLEALGCEWEPEQNGMFVWAKVPPLYADGFELSDEVLHGAL
                     VFITPGGIFGSRGNGYVRVSLCSDGAVLEEALRRIVEFKATGQSHK"
     gene            2411..>3149
                     /locus_tag="PK198_24905"
     CDS             2411..>3149
                     /locus_tag="PK198_24905"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRF42065.1"
                     /translation="MTITIIGIGLIGGSLAIALKENGFAERIIGCDKSSANIDKAIRR
                     RLIDDALPLEEAVAQSDIIIAATPVDAMVPMLPALLDLVDRQVVLELGSTKTAIVEAV
                     RQHPKRGRFVAAHPMAGTEYSGPEAAIPNLFDHKCTVLVDVEDSDTDAVATAEKLFRS
                     IPMRIVYLDAGAHDVHAAYISHISHISSFALALTVLAKEKDEERIFELAGGGFSSTVR
                     LAKSSPDMWAPIFRQNRDNVLDVLDEHI"
BASE COUNT          739 a          828 c          948 g          624 t
ORIGIN      
        1 cgtggtgatg cgcgagagca gtgcggccgg cgctaaaaag gcgcaattgc tgaccaatct
       61 ggggcagtac catcgccgcg agttccgcac ggcgaccaac ggagcggcca ctccgcagca
      121 aatttcaagc aacggtcgcc actggctgcg catcgtgcgg gtggggaacc agtttacctt
      181 gtacgcatcg ggcaacggca caagttggtt catcatcggc gctcaaaaca tcgtgatgag
      241 caactgcata gagctgggcc tggtagccac caatcagact gccaacagcg cggtgacggc
      301 gacgtttgcc aatatgagct tttccggaag caatagcacg ctgggaacaa acagcataga
      361 gcagagggca gagagcagag atatgccgca ctcctttgaa gtgtatccaa acccgacggg
      421 tggcgaactg aatgtggatt tgacgcagta tgtcggtcgc gatgtgcgga tagaggtgta
      481 cagcattgaa ggcaagttgt tgcagttcag agagatagag gaggtgcaga caacgatgga
      541 gaacctgaat ctgtcgcagt atgccggcgg gatgtatttt gtaaaaatca aatcgcacga
      601 tctgccggac gcgacgcggc gggtggtatt gcaacgggaa taacaggtcg gctatcgttg
      661 agggtagccc gttggggcaa ccctcgtggt tgccctgtaa attctgataa gtattggagg
      721 ataatagtaa tgccgcgaag ggaatgcctt cgcggctttt ttttgtgagg ggccgaatcg
      781 cgtggggttg cgccaggggc agtagcggaa tggcggcggt cggagataga gctgttagct
      841 ttaacaatgc aatagccgga taaagctaac agctctgcgg gggccgccat gtagcggata
      901 gcccggcccg cctgcctgat gcgggagggt ttggggagag caggtagtgg cgggggcgcc
      961 caaaaagaaa atcagaaaac agaagtgcaa acaaccataa gaacatcatg ctgaagcaaa
     1021 ataatccgat caccatgttt acgcccattg tgcagcccgc gcgaaggctg gccggcatca
     1081 acgagtacta tttttcggcc aaattgcgcg agatagccca gatgcgcgct gccggcaaag
     1141 acgtcatcaa cctcggcatc ggcagcccgg atttgccacc cgccgccgaa acagtggagg
     1201 cattggtgaa acacagccgc gagcgccaca cccacagcta ccaaagctac accggcatac
     1261 ccgaactgcg gcaggctttt gcccattggt acgggcagtg gtttggcgtg ccgctgaacc
     1321 ccgacaacga agtgctgccg ctcatcggct ccaaagaggg catcgtacac atagccatga
     1381 cctacctgag cgagggcgat caggcgctgg tgcccaatcc cggctacccc acctacaggg
     1441 cggctaccat cctgtcgggc gcggaaccac tgacctacga actgctgccc gaacaaggct
     1501 ggctgcccga tctggaagcg ctggagcagc aggatttgag ccgcgtaaaa attatgtgga
     1561 tcaactaccc caacatgccc accggcgctg cggctccgan nnnnnnnnnt tttttgaaaa
     1621 actgatcgcc ttcgcccgcc ggaaccgcat tctccttgtg aacgacaacc cgtattcgtt
     1681 catcctcaac gaccggcctg ccagcattct ggcggtggaa ggcgccaaag atgtggccat
     1741 cgaactgaac tcgctgagca aaagtaacaa catggccggc tggcgcgtgg gcgttttggc
     1801 cgggcgattt gactaccttc aggaggtgct gcgctttaag agcaacatgg attccgggat
     1861 gttcaagccg ctgcaattgg ctgctgcaca agcgcttgcc agtccgcccg aatggtacgc
     1921 acaactcaat acagtgtatc gcgagcggcg ggagaatgcg taccgactac tggaggcatt
     1981 gggttgcgaa tgggagcctg agcaaaacgg catgtttgtg tgggccaaag taccgccgct
     2041 gtacgccgac gggtttgaac tttcggacga ggtactgcac ggcgccttgg tgttcattac
     2101 gccgggtggc atattcggca gccggggcaa tggctatgtg cgggtgtcgt tatgcagcga
     2161 cggcgcggta ttggaggaag cgctgcggcg gattgtggag tttaaagcga cagggcagag
     2221 tcataaatag ctgcgagcta tgagccgcga gctgcgagtt gtgtttttca attttaaaac
     2281 gaagattacc gaattcatca aggttgagat tttcccaggc gggaaatttt catgcttcca
     2341 atataccaag ctcgtggctc gtaactcgcg gctcgcagct tttcaacaaa acattaattt
     2401 gcaacaaaaa atgaccatca ccatcatagg catcggactc atcggcggct cgctggccat
     2461 agcgttgaaa gagaacggct ttgcggagcg catcatcggc tgcgataaaa gcagcgccaa
     2521 catagacaag gccatccggc ggaggctcat agatgacgcc ttgcctttgg aagaggctgt
     2581 ggctcaatcg gatatcatca ttgccgctac gccggtggac gcgatggtac ccatgcttcc
     2641 cgcgctgctc gacttggtgg acaggcaggt ggtgctggaa ctcggctcca ccaaaacggc
     2701 cattgtggag gccgtgcggc agcatcccaa gcggggcagg tttgtggcgg cgcaccccat
     2761 ggccggcacc gagtattctg ggccggaagc tgccattccc aacctgtttg accacaaatg
     2821 caccgtgttg gtggacgtgg aagacagcga tacggatgcc gtagccactg ccgaaaaact
     2881 gttccgttcc atccccatgc gcatcgtgta tctcgatgcc ggggcgcacg atgtccacgc
     2941 tgcgtacatc tcgcacattt cgcacatcag ttcttttgcg ctggcgctca ctgttttagc
     3001 taaagaaaag gacgaggagc gcatattcga actggccggc ggcggtttca gttcgacggt
     3061 acgcttggcc aagagttcgc cggatatgtg ggcgccgatt ttcaggcaaa accgagacaa
     3121 tgtactggac gtattagacg aacacatcg
//
LOCUS       DAORLF010000509         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16876_length_3148_cov_2.058196, whole genome shotgun sequence.
ACCESSION   DAORLF010000509 DAORLF010000000
VERSION     DAORLF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16876_length_3148_cov_2.058196"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK198_24910"
     CDS             <1..266
                     /locus_tag="PK198_24910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRF42066.1"
                     /translation="HRQLAYEAGSIEALIQLVDHYEGVTIVPHLAVEQLSESKRGQVY
                     AFAHPIPSRTAGLVTRRNYPRIKILEALRAEIITALPPELRRL"
     gene            complement(405..1079)
                     /locus_tag="PK198_24915"
     CDS             complement(405..1079)
                     /locus_tag="PK198_24915"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HRF42067.1"
                     /translation="MNLFRGWIGEKKAAFYLWLSLSGKSYHIFNNIILPSRSGTTQID
                     HLIISVFGIFIVETKNKKGWIFGYKNQPNWTQVIYGEKYSFQNPLRQAFRQKKILSEF
                     LNLDESKIYTVIYFVGSCVFKTPLPENVINKGIGSLIKRFRSQVLSTEDVNRIIKLLR
                     KHLSESSLSNRDHIKSLRKRHSSNTLCPRCGSNLIERMAKSGYKAGTKFLGCENYPQC
                     KFTKDL"
     gene            complement(1175..2605)
                     /locus_tag="PK198_24920"
     CDS             complement(1175..2605)
                     /locus_tag="PK198_24920"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRF42068.1"
                     /translation="MEPISVFDMLKIGVGPSSSHTLGPWRAAERYVQELRDAEILPRV
                     ARLEAHLYGSLALTGKGHGTDIAILLGLSGADPVTIPVEDVRRIPEQIREETLLALGG
                     SHVIAFDPANHLVFWYNDALPFHANGLTFKSFDENTQILHEAVFYSIGGGFVVKENEE
                     RSADTVRLPYPIESGGQLAGYCAERQCSIWEIVQDNELSWRPTEETQAGLLKIWDVML
                     NCAYTGCHNEGVLPGGLNVVRRAPELHRKLLGAPTLPYSNAREWLGVIRNRPCNFNQI
                     LKWIGCFAMAVNEENAGFGRVVTAPTNGAAGVIPAVLLYYVCFSGQDVGDEEIIRFLM
                     VAGEVGSIFKKGSTISAAMGGCQAEIGVSSAMAAAALTEALGGSPDQALMAAEIAMEH
                     HLGLTCDPIGGLVQIPCIERNSMGAVKAITAAHIAVESDPANAKVSLDHVIKTMWETA
                     QDMNTKYKETSQGGLATQISIVLPEC"
     gene            2885..>3148
                     /locus_tag="PK198_24925"
     CDS             2885..>3148
                     /locus_tag="PK198_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42069.1"
                     /translation="MEASKETAGFIQAAVAAFENNAIVKLIISKPTAQAGDLKRIDAR
                     PVFIAGKPMLSFNFSFTTNDETKNHSSSGIYEVLSQQLGHHFLN"
BASE COUNT          748 a          864 c          758 g          778 t
ORIGIN      
        1 tgcaccgtca acttgcctac gaagccggca gcattgaagc gctcattcag ttggtggatc
       61 attacgaagg cgtcaccatc gtgccgcatc tcgccgtcga gcaattgtcg gagtccaaac
      121 gcggacaggt atatgccttc gcgcatccca ttcccagtcg cacggcgggc ttggtcacgc
      181 gtcgcaatta ccccagaatc aagatattgg aagcgcttcg tgcagaaatt atcaccgctt
      241 tgccgccgga gctgcgtcgc ttgtgagggg ggcggtatgc agtcggcagt ttgcagtggg
      301 cggtgcaaaa aaggaatgtg taatggttag gcgatttgaa gattgatagt tttaaagccg
      361 aagagctgcc ttaatggagt cgctgtagca tttccactct ctgattataa gtcttttgtg
      421 aatttacatt gcggataatt ttcacatccc aaaaacttcg tgccggcttt atatcccgat
      481 tttgccattc tctctataag attcgatcca catcttggac aaagggtatt tgaagaatga
      541 cgttttcgta aagattttat gtgatctcta ttggagagtg aagattcaga taaatgtttg
      601 cggagtaatt taataatgcg attaacatct tcagttgata atacctgact tcgaaatcgt
      661 ttgattaagc taccaatacc tttgttaata acattttcag gtaaaggagt tttgaataca
      721 cagcttccaa caaaataaat cacggtgtat atttttgatt catctaagtt caagaactca
      781 gatagaatct ttttttgccg aaaggcttgt ctgagtggat tctgaaaaga gtacttctcg
      841 ccatatatta cttgtgtcca gttgggttga tttttatagc caaaaatcca ccccttttta
      901 ttttttgtct ccactataaa gattccaaaa acagaaataa taaggtggtc tatttgagtt
      961 gtgccgctcc tcgaaggaag gatgatattg ttaaaaatgt gatacgattt accggatagt
     1021 gataaccaca aatagaaagc agcttttttt tctcctatcc aacctcgaaa tagattcatg
     1081 tcttttcctt tatggttata agtaagagct tcctgccggc gttaggttca tacctcgatc
     1141 caaatatcat gcgggttcca cccgcgctga cacctcagca ctccggcaac acaatcgaaa
     1201 tctgcgtggc caatcccccc tgcgaggtct ctttatattt cgtattcata tcctgcgccg
     1261 tttcccacat cgttttgatg acatggtcga gcgacacttt tgcgttggcc ggatcgctct
     1321 ccacagcaat atgtgccgcc gtgatggcct tcaccgcgcc catggaattg cgctctatgc
     1381 agggtatctg caccaagccg ccgatggggt cgcaagtgag acccaggtgg tgttccatcg
     1441 ctatttccgc ggccatcagc gcctgatcgg gcgagccgcc caatgcctca gtgagcgctg
     1501 ccgccgccat tgccgacgac accccgatct cggcctgaca acctcccatc gccgccgaaa
     1561 tggtagagcc ttttttgaaa atactgccca cctcgccggc caccatcaga aaccggatga
     1621 tctcctcatc gcccacatcc tgccccgaaa agcagacgta gtataacagc actgccggta
     1681 tcacgccggc cgcgccgttg gtaggcgccg tcaccactcg cccaaaccct gcgttttcct
     1741 cgttgacagc catggcaaag cagccgatcc acttcagtat ctgattgaaa ttgcagggcc
     1801 ggttgcgaat gacgccgagc cattcccgcg cattcgagta gggtagggta ggcgcaccca
     1861 gtagttttcg gtgcagttcc ggcgcgcgcc gcaccacgtt caaaccgccg ggcaacacgc
     1921 cctcgttgtg gcaacccgtg taggcgcagt tcagcatcac gtcccatatt ttcagcagtc
     1981 cggcctgtgt ttcctcagtt ggacgccacg acagctcatt gtcctgtaca atttcccaaa
     2041 tcgaacattg ccgttcggcg caatagccgg ccaattgccc gccgctctcg atggggtagg
     2101 gcaggcgtac cgtatcggcg ctgcgctcct cgttttcttt caccacaaaa ccgcccccga
     2161 tggagtaaaa aacggcttcg tgcagtattt gagtattttc gtcaaaactc ttgaatgtca
     2221 atccattagc atgaaaaggc agcgcgtcgt tgtaccaaaa aacgaggtgg ttggccgggt
     2281 caaaagcgat gacgtggctt ccgcccaagg ccagtaatgt ctcctcgcgt atttgttcgg
     2341 gtatgcgccg cacatcttcc actggaatcg tcaccggatc ggccccgctc agacccagca
     2401 ggatggcaat atcggtgccg tggcctttgc cggtgagggc cagcgatccg tacaagtgtg
     2461 cctccaaacg ggccacgcgc ggcagtattt cggcatcccg cagctcctgc acataccgct
     2521 cggcggcgcg ccaggggcca agcgtatgag aactggacgg ccccacgccg attttgagca
     2581 tatcaaaaac actaattggt tccatggtca agggtgaatt aagcctcaaa agtagtgatt
     2641 tttacaacct ggagcagcgg gaggttctcg gcgcggggaa cgtggaactt tgaacccgaa
     2701 acattgaact agtatgcgct gtgcggtagc cgcgccgtgg cgcggtaagc gcgagggaaa
     2761 cgcgcagtgc ctactcggcc agcagcttgt gccgctccgt ggcgcggtta tggcttgcag
     2821 ccgttccaac ccgaaacctt gaccccgaaa ccttgaaccc aaaccaatcc cctatctttg
     2881 ccgcatggaa gcatcaaaag aaaccgcagg cttcatacag gctgccgtag ctgcgtttga
     2941 gaacaacgcc attgtaaagc tcatcatcag caaacccacc gcccaagccg gcgacctgaa
     3001 acgcatagac gcccgcccgg tgttcatcgc cggaaagcct atgctgtcgt tcaatttcag
     3061 ctttaccacc aatgacgaaa ccaaaaacca ctcgtcttca ggtatttatg aagtcctttc
     3121 gcaacaactc ggccatcact ttctgaac
//
LOCUS       DAORLF010000510         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_16891_length_3146_cov_2.256875, whole genome shotgun sequence.
ACCESSION   DAORLF010000510 DAORLF010000000
VERSION     DAORLF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16891_length_3146_cov_2.256875"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..544)
                     /locus_tag="PK198_24930"
     CDS             complement(5..544)
                     /locus_tag="PK198_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42070.1"
                     /translation="MKGFIAIVFYLFPCAISAQEMDTTIFRDAANHTRALEYYLKQIE
                     TGHSDFHPYYQAACYASLLKNYDRAFVYLKEAIQKGARGEDVMTDTDFDVLKQDLTQW
                     NEIDSLLKEQYFQRYPGISKLDAGYELWLMWIEDQRYRTLKKNYKLDGAPTTDIAQHN
                     RHLLTMVRDILTPPRRRNN"
     gene            complement(575..1744)
                     /locus_tag="PK198_24935"
     CDS             complement(575..1744)
                     /locus_tag="PK198_24935"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HRF42071.1"
                     /translation="MKSIWIFFGVCIFWNTPMTAQLSGNEFTYTDLQTLCKTLGTEKT
                     IVGLGESTHGTKEFTSIRAEVVKNLVTQYHYRAFILEADYAPCEKINQYLSTGNGDIS
                     SLLVDLRLWPWIHEDFLKLLIWLKDYNLQNPLQKVQFYGMDSQYSRIFATKDAILLQY
                     PDWGRQLFEIVEGKEEPKVKITKLRSFDAQMVEGSDTIDLQRHYYVLCQINKLAQTIS
                     QNKNIRDENMARLVQLIQQQNGDGAKAMIWAHNSHISKQGTRVNEPTALGHHLAALYG
                     KAYSAVGLEFLEGEFLAVDYENAKERALLTFSLHPTQTTLAAAIDFGGKDIRVVSCYD
                     LRKKYYLNSIGAVYVAQPGKINSFYSKIEKDKEYDYLVVSPKSSAINVLPQYFNK"
     gene            complement(1821..>2679)
                     /locus_tag="PK198_24940"
     CDS             complement(1821..>2679)
                     /locus_tag="PK198_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRF42072.1"
                     /translation="RETPVVLHAAGPFMHTARPMLEACLRTGTHYLDITGEISVFEMA
                     ARLDAKAKAAGIMLLPGAGFDVVPTDCLAVHLKNQMPDATHLQLAFAGLGGGVSQGTA
                     TTMAENLGEGGAVRKDGKIVRVPTGHKTMIVPFKAKPLFVMSIPWGDVSTAYHSTGIP
                     NIETYMAFSPSAYRWVKMQRWFNWLLRTNFVKNIVKGRIRQRPAGPSPERRAKARSFV
                     WGRVTNAAGATLEAQLVTLEGYTLTAVTSLLIAQKVLAGNAPAGFQTPAKAYGADLIL
                     EVEGTERMG"
     assembly_gap    2680..2757
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2758..2888)
                     /locus_tag="PK198_24945"
     CDS             complement(<2758..2888)
                     /locus_tag="PK198_24945"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42073.1"
                     /translation="MKFLLYGATGYTGQLIAEYAAGFGLQPILAGRSADKLRAMAEQ"
     gene            complement(2951..>3146)
                     /locus_tag="PK198_24950"
     CDS             complement(2951..>3146)
                     /locus_tag="PK198_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42074.1"
                     /translation="AMDQHVDHGLPAEEAARRIERLFRVPKLKSRYAVGPGSQVLSVL
                     LKKILPASWMERILAGYAKV"
BASE COUNT          730 a          742 c          755 g          841 t
ORIGIN      
        1 ttttctaatt attacgccgc ctgggcggcg tgagtatatc acgaaccatt gttaacaaat
       61 gcctgttgtg ttgtgctata tccgttgttg gcgctccatc taatttatag ttctttttca
      121 acgtccggta tctttggtct tctatccaca tcagccaaag ctcataccct gcatctaact
      181 ttgaaatgcc cggatagcgt tgaaaatatt gctctttcaa caaggaatca atttcattcc
      241 attgggtcaa atcctgtttt aaaacatcaa aatccgtgtc tgtcattaca tcttcgcccc
      301 tcgcgccttt ttggatggcc tcttttagat agacaaaagc cctgtcgtaa tttttcaaca
      361 gtgacgcata acaggccgcc tgataataag gatgaaaatc tgaatggccc gtttcgattt
      421 gtttcaaata atactccaat gcacgtgtgt ggttggctgc atcccggaag atggtcgtat
      481 ccatttcctg agcagaaatc gcacatggaa acaggtaaaa aacaatggcg atgaaacctt
      541 tcataattgg aaatgatgta gagccgtact tgaattattt attgaagtac tgcggtaaaa
      601 cgttgatagc gcttgatttg ggggaaacaa ccaaataatc gtattcttta tctttttcaa
      661 tttttgaata aaaggaattg atttttcccg gttgagcgac ataaacagct cctatcgaat
      721 tgaggtaata cttcttcctc aaatcatagc aagacacaac acgaatatct ttgccgccaa
      781 aatcaatggc agcagctaag gttgtttgag tgggatgtag tgaaaaagtc agcaatgcac
      841 gttccttggc attttcatag tcaacagcca aaaattcgcc ctccaaaaat tccagcccga
      901 cagcactgta agccttgccg tacaaggcgg cgagatgatg cccaagcgcc gtaggttcgt
      961 tgactcttgt gccttgtttg ctgatatgac tgttgtgcgc ccatatcatc gcttttgcac
     1021 cgtcgccatt ttgttgttgt atcaattgaa ccagcctagc catattctca tctctgatgt
     1081 ttttgttttg tgaaatggtt tgggccaact tattgatttg acataaaacg taataatgcc
     1141 gttgcaaatc aatggtatct gatccctcca ccatttgggc gtcaaaactt cgaagctttg
     1201 ttatcttgac ctttggttct tcttttccct ctacaatttc aaaaagttgc ctgccccagt
     1261 ccggatattg caggaggatg gcatcttttg tggcaaatat tctggagtat tggctgtcca
     1321 ttccataaaa ctgcaccttt tgcagcgggt tttgcaggtt gtaatctttc aaccaaataa
     1381 gcagttttag aaagtcttca tgtatccagg gccataaacg tagatcaacc aataatgatg
     1441 agatgtctcc attgccggtt gacaaatatt ggtttatttt ttcacaaggc gcataatcag
     1501 cttccaaaat aaatgctcta taatggtatt gagtgacaag gttttttacc acctcagccc
     1561 tgatggatgt aaattccttg gtgccatgag tagattctcc gaggcccact attgtcttct
     1621 cagtgccgag cgtcttacaa agagtttgca agtcggtgta agtaaattca ttgcctgaaa
     1681 gctgtgccgt catgggcgta ttccaaaaaa tacaaacccc aaagaatatc catattgatt
     1741 tcatttcttg attagtttca atgtatgttc ttttacagct tcttgtcaag cccatctcca
     1801 atctttggta aataaaataa ctaccccatc ctttccgtgc cctccacctc cagaatcagg
     1861 tcggcgccgt aggctttggc gggtgtttgg aagcctgcgg gcgcgttgcc ggccagcact
     1921 ttttgggcaa tcagcagact ggtgacagcg gtgagcgtgt agccttcgag ggtgaccaac
     1981 tgtgcctcca gcgtagctcc ggcggcgttc gtcacgcggc cccacacaaa gctcctcgct
     2041 ttggcgcggc gttcgggcga agggccggcc gggcgttggc ggatgcggcc tttcacgatg
     2101 ttttttacaa aattggtgcg cagcagccag ttgaaccagc gctgcatttt cacccagcgg
     2161 taggccgagg gcgaaaatgc catgtaggtt tcaatgttgg gaatgccggt gctgtggtag
     2221 gccgtggaca cgtcgcccca ggggatgctc atcacaaaca ggggcttggc cttgaacggc
     2281 acgatcatgg ttttgtggcc cgtgggaacg cgcacaattt tgccgtcttt gcgcacagcg
     2341 ccgccttcgc cgaggttttc ggccatggtg gtagccgtgc cctgcgatac cccgccgccc
     2401 aatccagcaa aagccaattg gaggtgcgtg gcgtcgggca tttggttttt gagatgcacc
     2461 gccagacaat ccgtcggcac cacatcgaag ccggcgccgg gcaagagcat gatgccggca
     2521 gctttggcct tggcatccaa gcgggcggcc atttcaaaaa cggaaatttc gccggtgatg
     2581 tccaaataat gcgtgccggt gcgcaggcag gcttccagca tgggccgggc cgtgtgcatg
     2641 aaggggccgg cagcgtgcag caccacggga gtctcgcgcn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     2761 tgctcggcca tagccctgag cttgtcggca gagcgaccgg ccaaaatggg ttgcaatccg
     2821 aagccggcgg cgtattcggc aatgagttga ccggtgtatc cggtggcgcc gtagaggagg
     2881 aatttcatgt ggtggttatt tttaaagtgg gtaataagct acgagccgcg agcttcgagc
     2941 ttcgagttat ttatacctta gcataccccg ccagaatgcg ttccatccag gaggcgggca
     3001 atatcttttt gagcaatacg gagagcacct gcgagcctgg cccgacggcg tagcggctct
     3061 tcaatttggg cacacggaac aagcgttcaa tacggcgggc ggcttcttcg gcgggcaaac
     3121 catgatccac atgctgatcc atggcg
//
LOCUS       DAORLF010000511         3126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17019_length_3126_cov_1.648649, whole genome shotgun sequence.
ACCESSION   DAORLF010000511 DAORLF010000000
VERSION     DAORLF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17019_length_3126_cov_1.648649"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PK198_24955"
     CDS             complement(<1..1398)
                     /locus_tag="PK198_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42075.1"
                     /translation="MKPFLSSDRPYYLAALLLLPPALLIHLGLMTFIDDESIRSLVAL
                     EMKLSGNYVTPTLNGEYYYNKPPLYNWILLVFFNLAGRFDEFTARIPTLLSLLGFAAT
                     VFVVFRPKYDTRTAFVNALVLITCGRILFWDSILGLIDICFSWVMFGLFMTVYHAFRK
                     GRFWQLFLLTYLLTAIGFLMKGLPALVFQACTLSAYFLYKREFRRLFSAAHFAGIGLL
                     ALIVGGYYLLYHQYNDLSNIFATLFDESLKRTGARFGWKDTVLHLFSFPLEMVYHFLP
                     WSLMVLYFLRRGLLKQVLQDDFITFNLIAFFANILVYWTSPEVYPRYLLMLAPLLFSA
                     FLYLHEDHAARKTIAFRVVEGAFLAFLLLIVAGSVAPVLLERTQGVPGLYWKTGLLFA
                     GSILLTVLFFKNKDKRLLIMVTALLLMRVGFNWFVLPDRNAEDWGNLCRQSTIEAGLQ
                     FRDRPMYLLGDTELQM"
     gene            1585..>2751
                     /locus_tag="PK198_24960"
     CDS             1585..>2751
                     /locus_tag="PK198_24960"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF42076.1"
                     /translation="MKFSYPLNKVLIFIFALFSIPILAQEQESLVVTRISVIDQRSPV
                     THIFVDDDNVKWVANSKGVFQLRSSDLANPLALTAGEQSLYALPGGNFDLKWSAADMN
                     AALGNILNNTNTISAAAYSSARDELWIGTSNSGLHLLKTQPALRKVETLNTSNSKLTS
                     NKILHIFIDKKGRKWVGTAQGVLIGGEGRWTLEQKYFAIHRSTEGPNGIWVAGEGQIW
                     QVDSRDRWLAVPLNDLVSEGEVKDIAVGADGKLWVASESIVRWDSETDKIDRFGPIQY
                     YTSQYATCIALDQDGALWVGTEDKGVFLIDKASALTINILKEKELSCTPGQNDAALRV
                     KVTGGKGPFTYRWDKGLNGEAPANLGPGLYSLTVTDSQGRTKSAKITIEDTRMTA"
     assembly_gap    2752..2887
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2888..>3126
                     /locus_tag="PK198_24965"
     CDS             <2888..>3126
                     /locus_tag="PK198_24965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42077.1"
                     /translation="NIARKLNTGAHSVTITDKNGCKATATVNIEQKAAALRANLTETQ
                     PIRCPGDRSAALVVEVFGGKGPFQFKWNQAGLSGE"
BASE COUNT          782 a          864 c          780 g          564 t
ORIGIN      
        1 catttgcagt tccgtatcgc cgagcaggta catcgggcgg tcgcggaatt gcagccccgc
       61 ctctatggtg gactgccggc agaggttgcc ccaatcttcg gcgttgcggt cgggcagcac
      121 aaaccagttg aatcccaccc gcatcaacag cagcgccgtc accattatca acagccgttt
      181 atctttgttt ttaaaaaaca aaaccgtcag cagaatactg cccgcgaaca gcaggccggt
      241 tttccaatac agccccggaa ctccctgcgt gcgttccaac aataccggcg ccacgctgcc
      301 cgccacaatg agcagcagaa acgccaaaaa tgcgccttcc accacccgaa aagcgatggt
      361 tttgcgcgcc gcatgatctt cgtgcaggta caaaaacgcc gaaaacagca ggggtgccag
      421 catgagcagg tagcgcggat acacctccgg cgaggtccaa tatacgagga tattggcaaa
      481 aaaagcgatc agattgaagg tgatgaaatc gtcttgcagc acttgtttca gcaggccccg
      541 gcgcaggaaa tacagcacca tgagcgacca gggtaaaaag tgatacacca tctccaacgg
      601 aaagctgaac aggtgcaaca ccgtgtcttt ccagccgaaa cgagcccccg tgcgtttgag
      661 cgattcgtca aaaagggtgg caaaaatatt gctcaaatca ttgtattgat gatacaacag
      721 gtagtagccg cccacgatga gcgccagcag gccgatgccc gcaaaatgcg ccgccgaaaa
      781 cagccgccga aattcgcgtt tgtacaaaaa atacgccgac agcgtacatg cctgaaatac
      841 caatgcaggc aagcctttca tcaaaaaacc gatggccgtg agcaggtatg tgagcaaaaa
      901 taactgccaa aaccgcccct tgcgaaacgc gtgatacacc gtcataaaca gcccgaacat
      961 cacccaggaa aaacagatgt ctatgaggcc gaggatggaa tcccaaaaca ggatgcgccc
     1021 gcaggtgatg aggaccagcg cattgacgaa ggccgtgcgc gtgtcgtatt tgggtcggaa
     1081 tacaacaaaa acggtggcgg caaatccgag caggctgagc agggtaggga tgcgggccgt
     1141 aaactcgtcg aaacgaccgg ccagattgaa aaaaacgagc aaaatccagt tgtacagcgg
     1201 gggcttgttg tagtaatatt cgccgttgag cgtgggtgtc acatagttgc ccgacagctt
     1261 catttctaag gctaccaacg agcgaatgga ctcgtcgtca atgaaggtca tcagccccag
     1321 atgaatgagc aatgccggcg gcagcagcag cagcgcggcg aggtaatatg gtcggtcgga
     1381 agagagaaat ggcttcacag gcagcgttta cgggggcaaa gctacgtttt tttgcctttg
     1441 gtgcgagcct gtctcagcgt ttgccggtaa ggcgccatcc ccacaaaagc ccgttcagac
     1501 attgccgtcc ttcatactct cgcccgaccc tttttcgtta ggaagcgtta tctattgcat
     1561 ttttagtttt aacgcatccc gacaatgaaa ttttcctacc cgctgaacaa agtgttgatc
     1621 ttcatttttg cacttttttc catacctatc ctcgctcagg aacaagaaag cctcgtcgtc
     1681 acccgcatct ccgtgataga ccaacgcagc ccggtcacgc acatttttgt ggacgacgac
     1741 aatgtgaaat gggtggccaa cagcaagggc gtgttccaat tgcgctcctc cgatctggcc
     1801 aatccacttg ccctgactgc cggagagcaa tcgctctacg cccttccggg cgggaatttt
     1861 gacctcaagt ggtcggctgc cgacatgaac gcagcactgg gcaacatcct caacaacacc
     1921 aacaccatca gcgccgccgc ttatagctct gcccgcgacg aactctggat aggcacctcc
     1981 aactccggcc tgcacctgct gaaaacacag cccgctttgc gcaaagtaga gacgctcaat
     2041 acctccaatt ccaagctcac ttccaataaa atcctccaca tttttattga taaaaaaggc
     2101 cgcaaatggg tgggcaccgc acagggcgta ctgatcggcg gcgaaggacg ctggacgctg
     2161 gagcaaaaat atttcgccat tcatcgcagc accgaaggcc ccaacggcat ctgggtggcc
     2221 ggagaagggc aaatctggca ggtggacagc cgcgaccgct ggttggccgt gccgctcaac
     2281 gatctggtct cggaaggcga ggtgaaagac atcgctgtgg gcgccgacgg caagctctgg
     2341 gtggcttccg aatccattgt gcgctgggat tccgaaaccg acaaaattga ccgcttcggc
     2401 cccatccaat actacaccag ccaatacgcc acctgcatcg cactcgatca ggacggcgcg
     2461 ctctgggtgg gcaccgaaga caaaggcgtg ttcctcatag ataaggcttc tgccctgacg
     2521 atcaatattt taaaggaaaa agaactgagc tgtacacccg gccaaaacga tgccgctctg
     2581 cgcgtgaaag tgaccggcgg caaaggccct ttcacttatc gctgggacaa aggactgaac
     2641 ggcgaagcgc ccgccaacct cggccccggc ctgtattcgc tcaccgtcac cgacagccag
     2701 gggcgcacga agtcggccaa aatcaccatc gaggatacgc gcatgacggc gnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnncaa catcgcccgc aaactcaata ccggcgcaca ttctgtcacc atcaccgaca
     2941 aaaacggctg caaggcgaca gccaccgtca atatcgaaca aaaagcggcc gcgctgcggg
     3001 ccaacctcac cgaaacgcag cccattcgtt gccccggtga tcgttctgct gctttggtgg
     3061 tggaagtttt tggcggcaaa ggccccttcc agttcaagtg gaatcaggcg ggtctgagtg
     3121 gtgaaa
//
LOCUS       DAORLF010000512         3124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17030_length_3124_cov_2.066145, whole genome shotgun sequence.
ACCESSION   DAORLF010000512 DAORLF010000000
VERSION     DAORLF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17030_length_3124_cov_2.066145"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PK198_24970"
     CDS             complement(<1..1394)
                     /locus_tag="PK198_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRF42078.1"
                     /translation="MYLEFISQLGYLIGSVCFVWGLKLLSSPDTARRGNIIAAVGMAA
                     AIIAILLAPMERGDNNFALIAGGLVLGGGLGLFMAYKVKMTGMPQMVSILNGLGGASA
                     VVIATVEMSHYYDGSRPMSDSQLLIPLATLLIGGIAFTGSLLAWWKLEEWINDNALTL
                     PKHNIFNLLLLAVIVALGVWLFIQGPEASFGLAMLFMGLSLLYGFSFVIPIGGADMPV
                     VISLLNSFSGLSAAAAGLIYGNNFMLVGGILVGASGTILTLLMCNAMNRSLLNVLVGG
                     FGGSSGPAKAQQGDLVIREVSLNDAAIQLFYSQSVVFVPGYGLAVAQAQKVVKEVDEI
                     LEKNGVDVKYAIHPVAGRMPGHMNVLLAEADVPYPKLLDLEEANDFIANADVAVIIGA
                     NDVVNPAAEDDPSSPIYGMPVLQVWKAKHVIVLKRSMRAGYAGIDNMLFFHPRNKMLF
                     GDAKDTLGKLVGEL"
     gene            complement(1398..1709)
                     /locus_tag="PK198_24975"
     CDS             complement(1398..1709)
                     /locus_tag="PK198_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRF42079.1"
                     /translation="MDGFIQFFQDYLLLIYLLIFTIILGFEVISKVPTVLHTPLMSGA
                     NAISGVVVIGGILLVRQSESEDVLSMALGMVAIVLGMINAVGGFAVTDRMLEMFKKKK
                     K"
     gene            complement(1761..2834)
                     /locus_tag="PK198_24980"
     CDS             complement(1761..2834)
                     /locus_tag="PK198_24980"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRF42080.1"
                     /translation="MKLGILKEQNDSRVAIVPTLLGKFKDLGLEICIEQNAGAKASCA
                     DEEYTANATVCSRKEVLQNSDIVVCINAPSDEDLALLRPETLVISQFQSFIDDKVAPK
                     LKGMNLRAFGMDVIPRTTLAQSMDVLSSMASIAGYKAVLDAASRLPRYLPMMITAAGS
                     IKPAKVLILGAGVAGLQAIATARRLGATVEVFDTRAAVKEEVQSLGARFVEVAGAKDE
                     SSAGGYAVEQSEEFLQRQRAEVADRASKADIVITTAQVRGRKAPILLTQETVEKMKRG
                     SVVVDLAASTGGNCELTQDRQVIVHNGVTIVGNSDLASEMPQDASFLYGNNLLNFMKL
                     FVKKGELNIDLQNEIIRGAYLTA"
BASE COUNT          752 a          844 c          861 g          667 t
ORIGIN      
        1 ttcagttcgc ccaccaattt gccgagcgtg tctttggcat cgccgaagag catcttgttg
       61 cgcgggtgga agaagagcat attatcaata ccggcataac cggcgcgcat actgcgtttg
      121 agcacaatga cgtgtttggc tttccagacc tgcaacacgg gcatgccgta aatcggactg
      181 gaaggatcgt cctcggcggc gggattcacc acgtcgttgg cgccgatgat gacggcgaca
      241 tcggcattgg caatgaagtc gttggcttcc tccaggtcga ggagtttggg ataaggcaca
      301 tcggcttccg ccaatagtac gttcatgtgg cccggcatac ggcctgccac cgggtgaatg
      361 gcgtatttga catccacgcc atttttttcc aatatttcgt ccacttcttt caccactttc
      421 tgggcctgcg ccaccgccaa accgtagcct ggcacaaaca ccaccgactg agaataaaac
      481 agttggatgg cggcgtcatt gaggctcacc tcgcggatga cgagatcgcc ctgttgcgct
      541 ttggccgggc cgctgctgcc gccaaaaccg cccaccagca cgttgagcag cgagcggttc
      601 atggcattac acatcaacag agtgaggatg gtgcccgatg cacccaccaa aataccgccc
      661 accagcatga agttattgcc ataaataagg cccgccgccg ccgccgaaag cccggagaag
      721 gagttcaaca gcgagatcac taccggcata tccgcgccgc cgatgggaat gacgaatgaa
      781 aaaccataca gcagagaaag tcccatgaac aacatggcaa gaccaaaact ggcttcgggg
      841 ccttgtatga acaaccaaac acccagggct acaatgacag ccagcagcaa gaggttgaaa
      901 atgttgtgct taggaagcgt cagtgcattg tcattgatcc attcttccaa tttccaccaa
      961 gccagcaaac tgcccgtaaa ggcgatgccg ccgatgagca gcgtggccaa tggaatgagc
     1021 agttggctgt cgctcatagg ccgcgagccg tcatagtagt gcgacatttc tacggtggca
     1081 atcaccactg ccgaagcgcc gcccaaacca ttgagaatag acaccatttg cggcataccg
     1141 gtcattttga ctttgtaagc catgaagagt ccgaggccgc cgcccaatac caaaccgccg
     1201 gcaatgaggg caaaattgtt gtcgccgcgc tccattggcg ccagcaaaat ggcgatgatg
     1261 gcggctgcca tacccactgc ggctatgatg ttgcccctgc gggcggtgtc gggcgaactg
     1321 agcagtttga ggccccagac aaaacagaca gaaccgatga ggtatccgag ttgtgatata
     1381 aattcgaggt acatggctta ctttttcttt ttcttgaaca tttccagcat ccggtccgta
     1441 acggcgaacc cacccacggc attgatcatg cccaaaacaa tagccaccat gccgagggcc
     1501 atggaaagca catcttctga ttccgactgc cgcaccaaca aaataccgcc aatgaccacc
     1561 acgccgctga tggcgttggc gcccgacata agcggggtgt gtagtacagt gggcactttg
     1621 gaaatcacct cgaagccgag aatgatcgta aagatcagca gataaataag caggaggtaa
     1681 tcctgaaaaa attgaataaa accatccata ttaccggtgt tttatcggtg agtgaagagg
     1741 attctttata gcgggaggca tcatgcggta aggtacgcgc cgcgaatgat ctcgttttga
     1801 agatcaatat tgagttcgcc tttttttaca aataacttca tgaaattcag caggttgttg
     1861 ccatacagaa agctggcgtc ctgcggcatt tcagaggcga gatcggaatt gcccacgatg
     1921 gtgacgccgt tgtgaacgat cacctggcga tcctgcgtca gttcgcagtt gccgccggta
     1981 gaggctgcga ggtccacgac tacggagccg cgtttcatct tttcgacggt ttcttgggtg
     2041 agcaaaatcg gcgctttgcg gccacgcacc tgagccgttg tgatgacgat gtcggctttg
     2101 gaagcgcggt cggctacttc ggcgcgctgg cgctggagga attcctcgct ttgctccaca
     2161 gcataaccgc cggcagaaga ttcgtcctta gcgccggcca cttccacaaa acgagcgccc
     2221 aaactttgca cctcctcttt cacggcagca cgggtatcga agacttccac ggtagcaccc
     2281 aaacggcggg cggtggcaat ggcctgcaaa cctgctaccc cggcgcccaa aatgagcact
     2341 ttggcaggtt tgatactacc ggcagcggtg atcatcatcg gcaggtagcg gggcaagcgg
     2401 gatgcggcat ccaaaacggc tttgtagccg gcaatggagg ccatagaaga cagcacgtcc
     2461 atagactgag ccagcgtggt gcgcggaatg acgtccatac caaaagcgcg caggttcatg
     2521 cccttgagct tgggggccac tttgtcgtca atgaaagatt ggaactgaga aatgacgagg
     2581 gtttcggggc gcaacagcgc caggtcttcg tcggatggcg cgttgataca caccacaata
     2641 tcgctgtttt gcagtacttc tttgcgggag cagaccgtag cgttggcggt atattcttca
     2701 tcggcgcaag aggcttttgc tccggcgttt tgttcgatgc agatttccag acccaagtcc
     2761 ttgaattttc cgaggagtgt cggcacgatg gccacccggc tgtcattttg ttcttttaaa
     2821 attcccagtt tcattggcat tttcgtttac agggcgtgaa aatagaacaa acgttcgtaa
     2881 tccaaaaaaa acttttgtca ccaccagcgg taagttttgt catctgctgc gcgaaaacca
     2941 gtaggcggta ggcagtgggc ggtgtgcagt tctaagcgta gagtgggcgc gtggttgcag
     3001 cttgaagcca gcccgttcca acccggaacc cggaaccagt ggccgcgccg tagcgcgggg
     3061 taggcagtgc taagcgcgag ggaaacgcgt cttgcggctt gaagccagta gccagcagcc
     3121 accc
//
LOCUS       DAORLF010000513         3123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17042_length_3123_cov_2.218057, whole genome shotgun sequence.
ACCESSION   DAORLF010000513 DAORLF010000000
VERSION     DAORLF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17042_length_3123_cov_2.218057"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2603)
                     /locus_tag="PK198_24985"
     CDS             complement(63..2603)
                     /locus_tag="PK198_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF42081.1"
                     /translation="MKKSLLLSLLLLGSVAVMSQQSGKAYISGLVRDAVSEAPIELVT
                     VYIKGTNTVVETAANGRYRLEVPVNEEAVIVFSRIGYKEAQYELKPMPPNTTRQVDVS
                     LASTDTGLEVVVRDSRIQESGMVREDMAQLKLLPTTTGNLESVLPHIALGASSGSGGE
                     LTSQYNVRGGNYDENLVYVNDFEIYRPQLIRAGQQEGLTFPNIDLINSLSFSSGGFEA
                     RYGDKMSSVLDVKYKRPDSLRASVSAGFLGGSAHVEGSLLPMGENGPKRLRYLLGARY
                     KTTRYLLGTLDVKGEYVPNFSDIQAYVTYDLNRDWQLGYLGNLNRSVYRFAPTERSTA
                     FGLVDYALQLFSVFEGQEVDDFTTAMNGLSLTYLPDRSRNPFFLKLLVSRFQSDENER
                     FDIIGRYSLRQIDSNLGSDSFGEVLAELGNGTQHQYVRNFLDIRLHNAELKGGLELQQ
                     MYNDAKKSGTHFLQWSLKFQNEDIEDRINEWERLDSAGYSLPYDTSQVLLRNVLKTRN
                     ELNSNRFSAFFQDTYTVRRDDRGEMRLSLGLRASYWDLNGELLLSPRAQLLYKPLGGK
                     RDVSWRLAGGLYMQPPFYREMRGFDGLVNTQLRAQKSAHIVGGMTYDFYMGKRNPTKF
                     KLITELYYKSLWDMVSYEIENVRIRYSGLNDASGYITGVDLRVNGEFVPGAESWFNLS
                     LLRARERLDGVEHLRREVGQDAAEIVKDVPRPTDQLMSMSIFFQDYLPKNENFKMNLN
                     FTMGTGLPFGLQGNNRVYRNTYRFDAYHRVDIGFMLLMWDEARRAQRPHHPLRFSRQS
                     WLALEVFNLMQVQNQAGNTWIKTIFEQQYAIPNYLTSRRLNLRLRVDF"
     gene            complement(2622..3044)
                     /locus_tag="PK198_24990"
     CDS             complement(2622..3044)
                     /locus_tag="PK198_24990"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRF42082.1"
                     /translation="MIQRIQTIFLFLAALSNAGLFALPWAASAAPTEAGVYADGRLGA
                     GDHIAITVAVIAATVLALAAIGLFKNRKLQVQITSFSLLLAVAAGAFGGWLYYSAGQA
                     AAVQPGWLMLAPVVVFSMLAVRYIRKDEKLVRSADRLR"
BASE COUNT          750 a          837 c          847 g          689 t
ORIGIN      
        1 cgagcagcgg aaatcttaca aatcataaag atcagcgtcg gaaaaatcga tgctgttttt
       61 catcaaaaat ccacccgcaa acgaagattc aaacgcctcg aagtgaggta gttgggaatg
      121 gcatattgct gctcgaaaat ggttttgatc caggtattgc ccgcctgatt ttgcacctgc
      181 atcaggttga aaacctccag cgccagccag ctctgccgac tgaaccgcag tgggtgatgg
      241 gggcgctgag cacggcgggc ttcatcccac atcaggagca taaacccgat gtccacccgg
      301 tggtacgcat caaaacggta ggtattgcgg tatacgcggt tgttgccctg caaaccaaac
      361 ggcaggcccg tgcccatcgt gaagttgagg ttcattttga aattttcgtt cttcggcagg
      421 taatcctgaa agaagataga catggacata agttggtcgg tcggtctcgg cacgtctttg
      481 acaatctcgg cggcgtcctg tcctacctcg cggcgcagat gttctacacc gtccaagcgc
      541 tcgcgggcgc gcaacagcga caggttgaac caggattctg cgcccggcac aaattccccg
      601 ttgacccgca aatccacacc tgtgatgtaa cccgaagcgt cgtttagccc cgaatagcga
      661 atgcgcacgt tttcaatttc gtacgacacc atatcccata aactcttgta atacagctcg
      721 gtaatgagtt tgaatttggt aggattgcgc ttgcccatgt aaaaatcgta ggtcatgccg
      781 cccacgatgt gcgccgattt ttgcgcccgg agttgcgtat tcaccaagcc gtcgaaacct
      841 cgcatttccc ggtaaaaagg aggctgcatg tacaagccgc cggccaatcg ccaggacacg
      901 tctcgcttgc cgcccagagg tttgtacagc aattgcgcgc gcggagaaag gaggagttcg
      961 ccgttcaaat cccagtacga agcccggagg cccagcgaga ggcgcatttc tccccggtcg
     1021 tcgcggcgca cggtgtaagt atcttgaaaa aaagcgctga aacgattgga attcaattca
     1081 ttgcgggttt tcaaaacatt tctcaacaaa acctggctgg tatcataagg cagcgaataa
     1141 ccggcagaat ccaagcgttc ccactcgttg atgcggtctt cgatgtcttc gttttggaat
     1201 tttaggctcc actgcaaaaa gtgcgtaccc gatttttttg cgtcgttgta catctgttgc
     1261 aattccagac cgcccttcag ttccgcattg tgcagcctga tgtctaagaa attgcgcaca
     1321 tattggtgtt gggtgccgtt gccgagttca gccagcactt cgccgaagct gtcgctaccg
     1381 aggttggaat caatttgccg gaggctgtac ctgccgatga tgtcgaaacg ctcgttttcg
     1441 tcgctttgaa aacgagaaac caacagcttg agaaagaatg gattgcgact cctgtcgggc
     1501 agataggtca gtgaaagccc gttcatggcc gtggtaaaat cgtccacttc ctgcccttca
     1561 aaaacgctga acaattgcag ggcgtaatcc accaaaccaa aggcagtgct gcgctcggtg
     1621 ggcgcaaagc gatatacact gcggttgaga ttgccgaggt agcccaactg ccagtcgcgg
     1681 ttcagatcgt aggtcacata agcctgaatg tcggagaagt tgggtacata ttcgcctttc
     1741 acgtccaagg ttcccagcag gtagcgcgtg gttttgtaac gcgcgcccaa gaggtagcgc
     1801 aggcgtttgg ggccattctc gcccatcggc aaaaggctgc cttccacatg cgccgaaccg
     1861 cccagaaaac cggcgctcac ggaggcccgc aggctatcgg ggcgtttgta ttttacatcc
     1921 aaaactgagg acattttgtc gccatagcgg gcttcaaaac caccggaaga aaaggacaaa
     1981 gagttgatga ggtctatgtt gggaaaggta aggccctcct gctgacctgc gcggatgagt
     2041 tgggggcggt aaatctcgaa gtcgttgaca tacaccaagt tttcgtcgta gttgccgccg
     2101 cgcacattgt actgcgaggt gagttcgccg cccgatccgc tgctggcgcc cagggcgatg
     2161 tgcggcagta cgctctccaa gttgccggtc gtggtgggca gcaatttgag ttgggccatg
     2221 tcttcgcgca ccatgcccga ttcctgaatg cggctgtcgc gcaccactac ctccaaaccg
     2281 gtatctgtgg aagccagcga gacgtccacc tgacgggtcg tgttgggcgg catgggcttc
     2341 agttcgtatt gggcctcttt gtatccaatg cgcgaaaaaa cgatgacggc ctcttcattc
     2401 accggcactt cgaggcgata ccgaccattg gccgcagttt cgaccaccgt gttggtacct
     2461 ttgatgtaaa ccgtgaccag ctcaatgggc gcttctgaaa cggcgtcgcg cacgagaccg
     2521 gatatgtagg ccttgccgct ttgctgcgac ataacggcca cagaaccaag caacaagagg
     2581 gaaaggagga ggcttttctt cataattaag gtggatggta attacctcaa acggtcggct
     2641 gagcgaacca atttctcatc cttgcggata taacgcaccg ccagcatgga gaacaccacg
     2701 accggggcca gcatcaacca accgggctgc actgccgcag cttgtcctgc cgagtagtag
     2761 agccagccgc cgaatgcgcc ggccgcgacc gccaacaaga gggaaaagga ggtaatctgg
     2821 acttgcagtt tcctgttttt gaacaatccg atggccgcca atgccagtac cgtcgcagca
     2881 atcaccgcaa ccgtgatggc gatgtggtct ccggcgccca aacgcccgtc ggcatacacg
     2941 ccggcttctg tgggagcagc gcttgccgcc cagggcaaag cgaaaaggcc ggcattgctc
     3001 aacgcggcca aaaacaaaaa aatggtttga attctctgaa tcataaatgg ttatactttt
     3061 acagactaag gggcgataaa aaataaagtg gtcattttcg ccaaaggatt agataagtaa
     3121 acg
//
LOCUS       DAORLF010000514         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17157_length_3107_cov_1.961664, whole genome shotgun sequence.
ACCESSION   DAORLF010000514 DAORLF010000000
VERSION     DAORLF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17157_length_3107_cov_1.961664"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PK198_24995"
     CDS             complement(<1..726)
                     /locus_tag="PK198_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42083.1"
                     /translation="MAKEIIGNEYWDIADVIALVIVIGLLLLCIILSLRDKEIEVTSR
                     SLTLKNRFGKKVIPAKDIQGYKEEKFIPRIGGQAQLIFKANNRYYVIEGASCLNYLGI
                     VKFVREHYRELSDDHFRRYYFIRSIGIRLLFIVAGTMFTYVFFHNRSLDIAQWDKDVE
                     LVPLTLSEQPHIKVGGKKVRNYLRFFAKEYPEFEFNINGFRYKSCERTTITSLEKGDT
                     IMVGIQSSDVETKLKKSIPPSFWT"
     assembly_gap    989..1014
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1093..>1375
                     /locus_tag="PK198_25000"
     CDS             1093..>1375
                     /locus_tag="PK198_25000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF42084.1"
                     /translation="MNIQQNIRVAVDAIVFGYSRRQLHVLLIRQKFGLLKDRWALVGG
                     FVQDHEPLSEAVQRELREEAGISVDYLEQLYTFGDDVHRDPRMRVVSVAY"
     assembly_gap    1376..1402
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1403..>1749
                     /locus_tag="PK198_25005"
     CDS             <1403..>1749
                     /locus_tag="PK198_25005"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF42085.1"
                     /translation="YQLIADSDAEDARWFPIDELPPLAFDHARIIQTAYRRLQGKLSY
                     QPIGFDLLPEHFPFSDLENLYCTILNTEIDRRNFRKKMLSFGILEETEHSAIQKSGRP
                     GKLFKFNSLKYND"
     assembly_gap    1750..1766
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1875..2699
                     /gene="prs"
                     /locus_tag="PK198_25010"
     CDS             1875..2699
                     /gene="prs"
                     /locus_tag="PK198_25010"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRF42086.1"
                     /translation="MLLHLDPNFKPVAGEELAFEHFFFSGGEPHIKIHNAPTEGKVLI
                     THRIRSFNDLGLLCMAVDALRRSEVQHIELFIPYFPGARQDRVMVPGEPLSVKVYADL
                     INSLDLRRVYVFDPHSDVAPALLQRCAVIRNHDFIRQVLQHIGSPAALVSPDGGALKK
                     IFALSAALQIPEVVTCGKSRDVSTGQLTHFQVFADDLHGADCLIVDDICDGGSTFIGL
                     AEALRRHNAGRLFLAVSHGIFSRGTESLRTHFDTIFCTNAFRDMSAEGVVQIPIHL"
     gene            2711..>3107
                     /locus_tag="PK198_25015"
     CDS             2711..>3107
                     /locus_tag="PK198_25015"
                     /inference="COORDINATES: protein motif:HMM:NF037045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide phosphoribosyltransferase
                     domain-containing protein"
                     /protein_id="HRF42087.1"
                     /translation="MNPILLTDGYKLDHRRQYPKGTTMVYSNWTPRRSRIEGVEEVVF
                     FGLQYFLKKYVLEDFERHFFQQPKERVLAQYQRRVNCYLGENSVGIQHIADLHDLGYI
                     PMVFKAIPEGCSVPLRVPMFTMYNPLPEFF"
BASE COUNT          785 a          767 c          665 g          820 t
ORIGIN      
        1 agtccaaaat gaggggggta tggatttttt tagcttagtc tctacatcgc tggactgtat
       61 gcctaccata atggtgtctc ctttttctaa tgaagttatg gtggttcttt cgcagctttt
      121 atatcgaaac ccattgatat tgaattcaaa ttcggggtat tctttggcaa aaaatcgtaa
      181 gtaatttctg acttttttgc cccctacttt gatatgtggt tgttctgaaa gcgtaagcgg
      241 gaccaattcg acatctttgt cccattgagc gatatctaaa gacctgttat gaaaaaaaac
      301 ataagtgaac atcgtacctg ctactataaa gagtaaccga attccaatag agcgaataaa
      361 atagtatctt ctgaaatggt cgtcgcttag ttctctataa tgctctcgta caaacttcac
      421 tatgcccaag tagttgagac aagaagcccc ttcgattaca taatagcgat tgttcgcttt
      481 gaagatcaat tgagcttgac cacctatccg gggtataaat ttctcttctt tataaccctg
      541 aatatctttg gctggaatca ctttctttcc gaatctattt ttcaaggtaa gagacctgct
      601 ggtcacttct atctctttgt ctctcaagga taaaatgata cacagcaata ataaccctat
      661 tactatcaca agggcaatta catcggcaat atcccaatat tcattcccaa ttatctcttt
      721 agccaagagc aaagcgatga agatcaatac cgggagaatc cagatcgacc aacgtcgtat
      781 ttcgttgatg cattgagact tgatgcttga cgctttcttc attcgtgtag tgtcggggat
      841 atgccacctt caacgaaggc aatataacgc catcgccccc ttaccggcaa gtattgtcaa
      901 gaaaaaaata tcgaattgta tgtatttact taggaatgga gcattaaaaa atcacaatga
      961 accggataaa caaagcgggg acgtttttnn nnnnnnnnnn nnnnnnnnnn nnnntatgaa
     1021 aaattgagcg cggccttcaa atacccgcag attgtaagtt aatttgcgta ttcattacac
     1081 attagcttct acatgaacat tcaacaaaac atcagagtag ccgtggacgc catcgttttc
     1141 ggctacagcc gccggcaact gcacgtcttg ctcattcgcc aaaaattcgg cctgctcaaa
     1201 gaccgctggg cgctggtggg cggcttcgtg caagaccatg aacccctctc ggaggctgta
     1261 caacgcgaac tccgcgaaga ggccggcatt tcggtggact acctcgaaca gctctacacc
     1321 tttggcgacg atgttcaccg cgaccccaga atgcgcgtcg tttcggtggc ctattnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnaataccag ctcatcgccg attccgacgc cgaagacgcc
     1441 cgctggtttc ccattgatga actgccgcct ttggccttcg atcatgcccg catcatccaa
     1501 acggcttacc ggcgccttca gggcaaactc agctatcagc ccatcggctt cgatttgctg
     1561 cccgaacact tccctttctc cgatctggaa aacctctatt gcaccattct gaacacagaa
     1621 atagaccgcc gcaacttccg gaaaaagatg ctcagtttcg gcattctaga ggaaacagag
     1681 cactcggcga tacaaaaaag cggtcgcccc ggaaaattat ttaaattcaa tagtctgaaa
     1741 tacaacgacn nnnnnnnnnn nnnnnngact tgaaagaaaa gggttttgtt tttgaaataa
     1801 aatttgcgta aaaattacac aaaataaaaa tttcgccctt atatttgcgt cattattacg
     1861 caaataaaaa actcatgctg ttacatcttg atccaaattt caaacctgtt gccggcgagg
     1921 aactcgcttt cgagcacttt tttttctccg gtggagagcc gcacatcaaa atccacaacg
     1981 caccgaccga aggcaaagtg ctgattaccc accgcatccg ttctttcaac gatctcggcc
     2041 tgctgtgtat ggctgtggat gcgctgcgca gatcggaggt acaacacata gagttgttca
     2101 ttccttattt tccgggagcg cggcaagatc gggtgatggt tccgggcgag ccgctcagcg
     2161 tcaaggtata tgccgacctc atcaacagct tggacctccg ccgcgtctat gttttcgatc
     2221 ctcattccga tgttgcgccg gcactgttgc agcgttgcgc ggtcattcgc aaccacgatt
     2281 tcattcggca ggtgctgcaa cacatcggca gcccggcagc cttggtctcg cccgacggag
     2341 gcgcactcaa aaaaatattt gcattatctg ctgcattgca aatcccggaa gtcgtcacct
     2401 gtgggaaaag ccgggatgta tctaccggac aactcactca ttttcaggtt tttgccgatg
     2461 accttcatgg cgcggactgc ctcattgtgg acgatatctg tgatggcggg agcaccttca
     2521 tcggcctggc ggaggcactt cgccggcaca acgcaggtcg ccttttttta gcggtcagtc
     2581 atggcatttt cagtcggggc acagagtcgc ttcgtacgca tttcgatacc attttttgta
     2641 ccaatgcttt tcgcgatatg agtgcggagg gcgtagttca aattcccatt catctctaaa
     2701 acagtcattt atgaatccta tcctcctcac tgacggatac aagctcgacc accgacgcca
     2761 gtatccgaag ggcactacta tggtgtattc caactggacg ccccgccgca gccgcataga
     2821 gggcgtggaa gaagttgtgt ttttcggctt gcagtatttt ctaaagaagt acgttttgga
     2881 agacttcgag cgccattttt ttcaacagcc caaagagcgg gtactggccc aatatcaacg
     2941 ccgcgtcaat tgctacctcg gtgaaaactc ggtgggtatc cagcacattg ccgacctgca
     3001 cgacctgggg tatataccta tggtattcaa ggccataccc gaaggttgca gcgtgccttt
     3061 gcgcgtgcct atgttcacga tgtacaaccc cctgccggaa tttttct
//
LOCUS       DAORLF010000515         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17170_length_3106_cov_1.625696, whole genome shotgun sequence.
ACCESSION   DAORLF010000515 DAORLF010000000
VERSION     DAORLF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17170_length_3106_cov_1.625696"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK198_25020"
     CDS             <1..187
                     /locus_tag="PK198_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRF42088.1"
                     /translation="FGAYTVESTEDLDGFKFNLGEERWVMIRPSGTEPVLRVYAQAAD
                     QAAVRHILDAARQTLLG"
     gene            208..1644
                     /locus_tag="PK198_25025"
     CDS             208..1644
                     /locus_tag="PK198_25025"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRF42089.1"
                     /translation="MKKKTHLGAWLKPGISVLIPLLIAFMPAEWLHIPGITPVEQRVL
                     ALFVLAALFWILEPIPIFATSVLIIVLELILISNAGLQPMRQDATGAALPNLIDYREI
                     MGTFASPVILLFLGGFFLAMSAVKFRLDQSLAAVLLRPFGQKPKRLLLGIMAITAVFS
                     MFMSNTATTAMMLAVLAPVLPLFEPGDRGRVAFVLGVPFAANIGGIGTPIGTPPNAIA
                     LKYLTGENTIGFGQWMSFGVPLVLVLLLGIWLLLTTLYPARTPALELKFKTRFLRTPK
                     AYIVYATFVVTVLLWLTDFWHGMNSYVVSMIPVAVFLSFGIIGKEDLKNISWDVLWLV
                     AGGIALGLALEKSGLATRLVTLIPFSGLSAMTVLAVAALVGLVMANFMSNTGTANLLL
                     PIVAALGTSGLALESTGGIAGIILGVTLSISLGMSLPISTPPNALAHAGGDIQTSDMA
                     RAGVIIGLAGLGIVFALIMILSGTGFFN"
     gene            1650..>2317
                     /locus_tag="PK198_25030"
     CDS             1650..>2317
                     /locus_tag="PK198_25030"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRF42090.1"
                     /translation="MKDSLVKINTEWLKSTYFSDTQKRIQLKAGEVLLRQNQHNERLF
                     LVIEGQLRGFLNYDSEEPFEAFRSAPNHFVGVYSYFSHTHQSYSTVIAETDCEVAYIE
                     GDQADVDGFGFAEHFLPVVVEEIYMRQLLTQKMTIQNQKTTRKLFHSEKMAMLGQLAA
                     GLAHELNNAVGVIKRQGEHLADWAQTLVQQGGDPSAQYLFRKGLEQGSSTSTADARQR
                     RRQD"
     assembly_gap    2318..2327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2328..3040
                     /locus_tag="PK198_25035"
     CDS             <2328..3040
                     /locus_tag="PK198_25035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF42091.1"
                     /translation="RRELEERFDLPSSVARQAAAAGLDDADMQSLGKDAAEKLEQFLP
                     YFDAGLAFHDLNHAADHASQVVRSVKELGVANHSRRIPVDIADTLRQATMLLKEELKG
                     IALDLKTTPAMMIEANPGEVVQIWVNLIKNACEALRESGRRKPTIAVEAVLKEGDKAE
                     VTITDNGPGIPPDLMPRIFQPDVTTKVEGLSFGLGLGLSIVQKIVDNLRGKIAVESEP
                     GRTVFTIRLPVAAKSPQS"
BASE COUNT          669 a          862 c          852 g          713 t
ORIGIN      
        1 tttcggggct tacacggtag aatccactga agatttggac ggtttcaaat tcaacctcgg
       61 cgaagagcgt tgggtgatga ttcgtccttc gggtaccgaa ccggtgctgc gggtgtatgc
      121 ccaggctgcc gaccaagccg ccgtgcgcca tatattggat gcggcccggc aaaccctgct
      181 ggggtgattt tgatctcaca aaaaaccatg aaaaagaaaa cacatctggg cgcctggctg
      241 aaacccggca tttccgtcct tatacccttg ctgattgcgt tcatgcctgc cgaatggctg
      301 cacattcccg gcatcacgcc cgtagagcag cgagtgctgg cgctgtttgt gctggccgcc
      361 ctgttctgga ttctggagcc gattcccatt tttgccacct cggtgctcat cattgttttg
      421 gaattgattc tcatttccaa cgcaggcctc caacccatgc ggcaagacgc gaccggagca
      481 gcattgccca atctgataga ctaccgcgag atcatgggta cctttgcctc gcctgttatt
      541 ttgctgtttc tggggggctt ctttctggcc atgtcggcag tcaaatttcg tttagaccaa
      601 agtctggcgg cggttttgtt gcggcctttc ggccaaaaac ccaagcgatt gctgctgggc
      661 atcatggcca taacggcagt gttttctatg tttatgagca ataccgccac caccgccatg
      721 atgctggcag tgttggcgcc ggtgctgccg ctgtttgaac ccggcgaccg ggggcgggtg
      781 gcatttgtgc tgggggtgcc ctttgcggcc aatatcgggg gcatcggtac gcccatcggt
      841 acgccgccca atgccattgc gctcaagtac ctcaccggag agaataccat cggtttcggg
      901 cagtggatga gcttcggggt gccgttggta ttggtgctgt tgttggggat atggctgttg
      961 ctcactaccc tgtatcctgc ccgcacgcct gcgttggagc tgaaattcaa aacccgcttc
     1021 ctccgcacgc ccaaagccta cattgtttat gccactttcg tggtaacggt gctgctgtgg
     1081 ctcaccgatt tctggcatgg catgaactcg tatgtggtgt ctatgattcc ggtggctgta
     1141 tttctcagct tcggtatcat cggcaaagaa gatttgaaaa acatcagttg ggatgtactc
     1201 tggctcgtgg ccggcggtat tgcgctgggt ctggctttgg aaaaaagcgg tttggccacc
     1261 cggctcgtga cgctcattcc cttttcgggg ctttccgcca tgacggtact agctgtggcc
     1321 gcattagtgg gcttggtaat ggccaacttc atgtccaaca ccggtactgc caacctcctg
     1381 ctgcccattg tggcggcact gggtacatcg gggctggcgc tcgaatccac cggcggcata
     1441 gcgggcatca tattgggagt tactttgagc atttctctgg gtatgtcgct gcccatcagt
     1501 acgccgccca acgccctggc ccatgccggc ggcgatattc agacctccga catggcacgc
     1561 gcaggcgtca tcataggtct ggcggggctg ggcattgttt ttgctttaat catgatattg
     1621 agcggcactg gctttttcaa ctgaaaaaaa tgaaagactc gctcgtcaag ataaataccg
     1681 aatggctgaa aagtacttat ttttcagaca cccagaagcg catccaactg aaagccggcg
     1741 aagttttgct gcgtcagaac cagcacaatg agcgcctttt tttggtcatt gaggggcaat
     1801 tgagaggctt tttgaactac gattccgaag agccgttcga ggcattccgt tcggccccca
     1861 atcactttgt aggcgtttac agctactttt cgcacaccca ccagagctac tccaccgtca
     1921 ttgcagaaac cgattgcgag gtggcctaca ttgaaggcga ccaggccgat gtggatggat
     1981 ttggctttgc cgagcatttc ctgccggtgg tagtggagga aatttatatg cgccagttgc
     2041 tgactcaaaa aatgaccatt caaaaccaaa agaccacgcg caaactcttc cattctgaaa
     2101 aaatggccat gctgggacaa ttggcagccg ggctggcgca cgaactcaac aacgcggtgg
     2161 gcgtcatcaa acggcagggc gaacatctgg ccgactgggc gcaaacgctg gtgcaacagg
     2221 ggggcgatcc aagcgcgcag tacctcttcc gcaaaggttt ggagcagggc agcagcacct
     2281 ctaccgccga tgcccggcag cgccgccggc aggaccannn nnnnnnngcc gccgcgaatt
     2341 ggaagagcga ttcgacctgc cctcctcggt ggcccggcag gccgccgccg caggtttgga
     2401 cgatgccgat atgcaatcat tgggcaaaga tgccgccgaa aaactcgaac agtttttgcc
     2461 ttatttcgac gccggattgg ccttccacga tctcaaccac gccgccgacc atgcctctca
     2521 ggtagtgcgc tccgtcaaag aattgggtgt ggccaaccac tcccggcgca ttcctgtgga
     2581 cattgccgac accctccgcc aggcgaccat gctgctcaaa gaagaactca aaggcatcgc
     2641 attggatttg aaaaccacac ccgccatgat gatagaggcc aatccgggcg aggtggtgca
     2701 gatttgggtc aacctcatca aaaatgcctg cgaagccctc cgcgaatcag gccgccgcaa
     2761 accgaccatt gccgttgagg ccgttctgaa agaaggcgac aaagcagaag tgaccatcac
     2821 cgataacggc cccggcattc cgcccgacct gatgccgcgc atttttcaac cggatgtgac
     2881 caccaaagtg gaggggctgt ctttcgggct gggattgggc ctgtccatcg ttcaaaaaat
     2941 agtggacaac ctgcggggca aaattgccgt tgaaagtgaa ccgggccgca cggtctttac
     3001 catccgattg cccgtcgcag caaagagtcc gcaatcttga atccgaaatc cggaatccga
     3061 aatcttgaat aaaaacgcta tggaaaaaac atacatcatc tgcgta
//
LOCUS       DAORLF010000516         3088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17290_length_3088_cov_2.209693, whole genome shotgun sequence.
ACCESSION   DAORLF010000516 DAORLF010000000
VERSION     DAORLF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17290_length_3088_cov_2.209693"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK198_25040"
     CDS             complement(<1..241)
                     /locus_tag="PK198_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42092.1"
                     /translation="MQIFVDSVGFTDINGMAIRTQYVTWTHPWFNYVDHSKIYEGIGS
                     APAIYFFWGSGETVDFFLHTFNCYESPTHGIFNFVG"
     gene            complement(287..625)
                     /locus_tag="PK198_25045"
     CDS             complement(287..625)
                     /locus_tag="PK198_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42093.1"
                     /translation="MKKLIFLLLMLIALAPAQAQLEWAPVGAKYYHSVADFMPGGGSS
                     PYSVYISEVVGDTIIAGKNCRVLDTWWLPGCEGREFTYEEEGRVYLYDEESGRFGLVF
                     DFNKGAGESW"
     gene            716..>907
                     /locus_tag="PK198_25050"
     CDS             716..>907
                     /locus_tag="PK198_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42094.1"
                     /translation="MKHFILSAFCLAFLSCNSVKKAERVFGSELFAHWVHSHEEDSAD
                     YRVFRRADYAFPPSRGREGF"
     assembly_gap    908..917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <918..1097
                     /locus_tag="PK198_25055"
     CDS             <918..1097
                     /locus_tag="PK198_25055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42095.1"
                     /translation="EIKSDGSFAHHQIGPVDAPVMVAGKWKLQGDNILRISPQTGDAW
                     TLQIVEVSKDVLKVK"
     gene            complement(1205..1510)
                     /locus_tag="PK198_25060"
     CDS             complement(1205..1510)
                     /locus_tag="PK198_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF42096.1"
                     /translation="MSKAKVVVNNDKLQYSLELLRALAHPLRLQILKFIDEHESINVN
                     KIYSTLNLEQSITSQHLKILRSAGLVITEREGKFIHYRIDYPKVQRAAENIHLFVQH"
     gene            complement(1625..1963)
                     /locus_tag="PK198_25065"
     CDS             complement(1625..1963)
                     /locus_tag="PK198_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF42097.1"
                     /translation="MRKQKTESVVLKQGLNDVQLDYAELRKAVLVLRAVNHKLRQRII
                     ALLEEGEGMNVTDIFVKLRLEQSVASQHLAILRRAGIVYTERSGKFIHYHLNKERLSQ
                     ISHLVEELLA"
     gene            2221..>2769
                     /locus_tag="PK198_25070"
     CDS             2221..>2769
                     /locus_tag="PK198_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF42098.1"
                     /translation="MDTQSLWYLENIDVNGIFCPQKLGRGDMDNHPHRQYAKGEYIYL
                     QEDEADKIFFLTEGRVKIGTTDAASGKEITKAILGKGEVFGELAAMGEGKRRDYACTM
                     EPTTVCILSAEDMQSLMRERSGLNLFLMRLISSRVLELEQRLTSLVFKDSRTRVMEYL
                     YHLGQTKGRAIGFEVLVNNFLNQ"
     assembly_gap    2770..2787
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2828..2917
                     /locus_tag="PK198_25075"
     CDS             2828..2917
                     /locus_tag="PK198_25075"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42099.1"
                     /translation="MRDKKILKFDRRRLLIHDMERLKGEIKGA"
BASE COUNT          811 a          775 c          729 g          745 t
ORIGIN      
        1 cgcccacaaa attgaaaatg ccgtgcgtgg gggattcgta gcagttgaag gtgtgcagga
       61 aaaagtcaac agtctctccg gatccccaaa aaaaataaat cgccggcgca ctgccgatgc
      121 cctcatatat tttggaatga tccacataat taaaccaggg atgtgtccag gtgacgtatt
      181 gtgtgcgaat ggccatgccg tttatatcgg taaacccgac ggaatccaca aaaatttgca
      241 tcgagtcggc aggtgtctct ccgaaccaat aactgcaaac cggtactcac caagattcgc
      301 ccgctccctt gttgaagtca aaaaccagcc cgaaacggcc cgactcttcg tcgtataaat
      361 aaacgcggcc ttcctcttca taagtgaatt ccctgccttc acatccggga agccaccagg
      421 tatccaacac tcggcagttc ttcccggcaa tgatggtatc gccgaccact tccgaaatat
      481 atacgctata cggagaacta ccaccacccg gcataaaatc tgcaacagaa tgataatact
      541 tcgcccctac gggcgcccac tccaattgcg cctgcgccgg agccagagcg atcagcatca
      601 acaagaggaa aatgagtttt ttcatggttg tgtgtttttg tgacggttca tcaatattcc
      661 ataagacgca ggtgaaaaag actatatttg caaggtctaa aaaaacctta aagccatgaa
      721 gcatttcatc ctttcagcct tttgtctggc gtttttgagt tgcaattccg tcaaaaaagc
      781 cgaacgtgtt ttcggaagcg aactcttcgc gcactgggta cactcgcacg aagaagacag
      841 cgccgattac cgcgtattcc gccgcgccga ttatgcgttc ccgccgtctc gtggccgcga
      901 ggggtttnnn nnnnnnngaa atcaaatccg acggcagttt tgcgcaccat cagatcgggc
      961 ctgttgacgc gcctgtgatg gtggcgggta aatggaaatt gcagggcgac aacatcctgc
     1021 gcatcagccc gcaaaccggc gatgcctgga cgctccaaat tgtagaggtg agtaaagacg
     1081 tactgaaggt aaagtaggtc ttgttacggg aggggcgacc gcctgccacc cctcccatac
     1141 tgccggaaca gccgcccggc ttattccgtc cggcgatccc cggccatcct tttggccccg
     1201 ccgatcaatg ctgcacgaac aaatggatgt tctctgctgc ccgctgcacc ttggggtagt
     1261 ctatccggta atggatgaat tttccctcgc gctcggtgat gaccaatcct gccgatctca
     1321 aaatcttcag gtgttgggag gtgatggatt gctccagatt cagagtgctg tagattttgt
     1381 tgacgttgat gctctcgtgc tcgtcaataa atttcaaaat ttgaagcctc aacgggtgcg
     1441 ccaatgcgcg caataactcc aacgaatatt ggagcttgtc gttgttcaca acaactttag
     1501 ccttactcat agcgatgttt gtgtagtttt caacaaagga gataaaaaaa atcgtcgtca
     1561 gtacttactt ggggggaaag tatgtcgaag tagaaccgca aaacaggcgc agcgcccttt
     1621 ttgctcaggc cagcaattcc tctaccagat gcgatatctg cgaaaggcgc tctttattca
     1681 aatggtagtg aatgaatttg ccgctgcgct cggtataaac gataccggcc cggcgcaaaa
     1741 tggccaaatg ctgggatgcc accgactgct ccaaacggag ttttacaaag atatcggtta
     1801 cattcatgcc ctcgccctct tccaaaagag cgatgatgcg ttggcgaagt ttgtgattga
     1861 cggccctcag aacgagcacc gctttacgaa gttcggcata gtctaattgt acgtcgttca
     1921 gcccctgctt gagtacaacc gactctgttt tttgttttct catggcgaaa gtattggtat
     1981 cggcattatt gcctctcaaa gtagtattca aagactgtac caaaaatttc aaatcttaat
     2041 ttttgtaagt ccgtagcgct ctggccgcaa agttacaaaa acatttgaat aatacaaata
     2101 tgtacaaaaa cattctgccg ttccataacg caatggagcc gttcgcattg catttggggc
     2161 atttttttgc cggagcgggc aaaagtttct accttagccg acgcaaatcg aaataacctt
     2221 atggatacgc aatcactttg gtatcttgaa aatatagacg tcaacggcat tttttgtccg
     2281 caaaaattag gtcgtggcga catggacaat cacccgcatc ggcaatatgc caaaggcgag
     2341 tacatctatt tgcaggaaga tgaagccgac aagatttttt ttctcaccga aggccgcgtg
     2401 aaaatcggta ccacagatgc tgcttccggc aaggaaatca ccaaggccat tctcggcaaa
     2461 ggcgaggtgt ttggagaact ggccgccatg ggagagggca aacgccgcga ttacgcctgc
     2521 acaatggaac ccaccaccgt gtgcatcctc agtgcagaag acatgcaatc gctcatgcgc
     2581 gagcgctccg gccttaatct attcctgatg cgcctcatca gttcgcgggt actggaactg
     2641 gaacagcggc ttacatctct ggtttttaaa gactcccgca cgcgcgtcat ggagtatctg
     2701 taccatctgg ggcaaaccaa agggcgggcc atcgggtttg aggtcttggt caataatttc
     2761 ctcaatcagn nnnnnnnnnn nnnnnnncgc cacttcccgg caaaccgtca acaccgcgct
     2821 gaatgatctg agagacaaaa aaatactgaa gtttgaccgc cgccgcctgt tgatccacga
     2881 tatggagcgc ttgaagggag agataaaggg ggcatagata aagcgggctg caaacttgat
     2941 ttttgtaagg aaatcagata ttccgattgg gaaaccatat aacgcgaact gcttaaactg
     3001 tgaacgcaga ccattcatta taaaatagca actcagatga aaacaggctt tttgtccatt
     3061 tttgcctcct tgaccatcct gctgggct
//
LOCUS       DAORLF010000517         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17367_length_3076_cov_2.204568, whole genome shotgun sequence.
ACCESSION   DAORLF010000517 DAORLF010000000
VERSION     DAORLF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17367_length_3076_cov_2.204568"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="paaA"
                     /locus_tag="PK198_25080"
     CDS             complement(<1..737)
                     /gene="paaA"
                     /locus_tag="PK198_25080"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRF42100.1"
                     /translation="MHAKRSKIMQDLTLEHAFQSKIDADQKIEPRDWMPDIYRKTLIR
                     QISQHAHSEIVGMLPEGNWITRAPSLRRKVALIAKVQDEGGHGLYLYAAAETLGIDRA
                     TLIEELHSGKAKYSSVFNYQAITWADMGAIGWLVDGAAIMNQVALCRTSYGPYARAMV
                     RICKEESFHQRQGYEIMLTLAQGSPEQKAMAQDALNRWWWPALMMFGPNDKESLHTEQ
                     SMRWRIKRFTNDELRQRFVDMTVPQAE"
     gene            complement(843..>1135)
                     /locus_tag="PK198_25085"
     CDS             complement(843..>1135)
                     /locus_tag="PK198_25085"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRF42101.1"
                     /translation="VGDVSEDYQDEYQLVQRDENSWLVDGQYPIQEFLRYFNLHIEDQ
                     YDGQFLTVAGLMLSKFHALPKVGDKMNFDDLQLEVVDKDGQRIDKILVTRMK"
     assembly_gap    1136..1146
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1147..2110)
                     /locus_tag="PK198_25090"
     CDS             complement(<1147..2110)
                     /locus_tag="PK198_25090"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRF42102.1"
                     /translation="MEILIIIGLVLLNGIFSMAEMSLVSSRKFRLESSSKKGNKGAQA
                     ALRLSENPNRFLSTVQIGITLIGILLGVYSGENMTDDVTGFLSRFEWLRPYADTVAVF
                     IIVVFVTYLSIVLGELLPKRLGLTFPEPIAIVLAQPMLLLSKITAPFVWLLTKTSEIL
                     LRLMGIRSNMDSKVSEEEIKSIIKESAEGGEIQDIEQNIVERVFELGDLRVTSLMTHS
                     GDLVYFDENDDLKTVRRKVAEEKHSAYPVCADDDIDEILGIVLLKDLFEASSDEGFHL
                     KSHLKPPLFINESMPAYKMLELFKKERLHYAIVIDEFGSTSGMVT"
     gene            2324..2926
                     /locus_tag="PK198_25095"
     CDS             2324..2926
                     /locus_tag="PK198_25095"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRF42103.1"
                     /translation="MIIDLLFLITAIYGLYLGYSRGIIKTVFTVLSVLFGVIIAFRFS
                     PDMTELLESLFTSTNPLMFIAGFLLTFVLTMIAIRTFARVLENGLESANINFINQAAG
                     AAITAAFTTLLFSTLVLFANKAHLIEEDTKQDSITYSYLEKYPQTMWGLLGKAKPTLQ
                     RFWDRSLEFMDRLEDYRVEDSESEPTIYDIEEDEPTRTTQ"
BASE COUNT          746 a          760 c          739 g          820 t
ORIGIN      
        1 aattcggcct gcggcacagt catatccaca aagcgctgcc ttagctcgtc gttggtaaag
       61 cgtttgatgc gccagcgcat agactgctct gtgtgtaaag attctttgtc gttggggcca
      121 aacatcatca gggcgggcca ccaccagcgg ttgagcgcgt cctgtgccat ggctttttgc
      181 tccggcgaac cctgtgccag cgtgagcatg atctcgtagc cctggcgttg gtgaaaactt
      241 tcttctttgc aaatacgcac catagcgcgg gcatacggcc cgtaagatgt gcggcacagc
      301 gccacctgat tcataatagc ggcgccgtcc accaaccagc cgatggcgcc catgtcggcc
      361 caggtaatgg cctggtaatt gaatacgctg ctgtatttgg ccttgccgct gtgcagctct
      421 tcgatgaggg tagcccggtc aatgcccagc gtttcggcgg cagcgtacaa atagaggcca
      481 tgaccgcctt cgtcttgcac cttggcaatg agggccacct tgcgacgcaa agaaggagcg
      541 cgggtgatcc agttgccttc gggcaacata ccgacgattt cggaatgggc gtgctgggaa
      601 atctgacgaa tcaaagtttt ccggtaaata tccggcatcc agtctctcgg ctcgatcttc
      661 tgatcggcgt caattttaga ttgaaatgcg tgctccagcg tcaggtcttg catgatcttt
      721 gatcgtttgg cgtgcaaata tagatatttg tttcaaataa cgaacatttg ttcgtaatgt
      781 tcgtaaaaaa ctataccccc tcaccattag tagtggggca aaagccgttt ttcatcgccc
      841 ctttatttca tccgcgtgac caggatttta tcaatgcgct gcccgtcttt atcaactact
      901 tctaattgta gatcatcaaa gttcattttg tcgcctactt tgggcagggc atgaaacttg
      961 gaaagcatca gtccggctac cgtcagaaat tgcccgtcgt actggtcttc aatatgcagg
     1021 ttgaagtagc ggaggaactc ctgaatggga tattgcccgt ccaccaacca ggagttttca
     1081 tcccgctgca ccaattggta ctcatcctga taatcttcgc tgacgtctcc caccannnnn
     1141 nnnnnntagt gaccataccg ctggtggagc caaactcatc aatgacgatg gcgtagtgca
     1201 ggcgttcttt tttgaaaagt tccagcattt tataggccgg catgctttcg ttgataaaca
     1261 aaggcggttt gagatgcgat ttgagatgga agccctcgtc ggaagatgct tcaaaaaggt
     1321 ctttcaacaa aacgatgccc agaatttcat caatatcgtc gtcggcacaa acagggtacg
     1381 ccgagtgctt ttcttccgct actttcctgc gcacggtttt caagtcgtca ttttcatcaa
     1441 aatacacgag gtcgccggag tgtgtcataa gcgaagttac tctgaggtcc cctaattcaa
     1501 acacccgctc tactatgttt tgctcaatgt cctgaatctc gccgccctcg gcgctttcct
     1561 tgatgatgga tttgatttct tcttccgaca ctttgctgtc catgttgctg cggatgccca
     1621 tgaggcgcag aagaatttcg ctggttttag tgagcaacca tacgaacggc gccgttattt
     1681 tagacaacaa aagcatgggc tgtgccaaaa cgatggcaat gggttcgggg aaagtgaggc
     1741 ccagtctttt gggcaataat tcgccgagca caatggacag ataagtaaca aaaaccacaa
     1801 tgatgaaaac ggcgacggta tcggcataag gcctcaacca ttcaaaacgg ctcagaaatc
     1861 cggttacatc gtcggtcatg ttttcaccgc tatatacacc gaggaggatg ccgatgagcg
     1921 tgatgccgat ttgtacggtg gacaaaaagc ggttggggtt ttcggacagt cgcaaagctg
     1981 cctgtgcgcc tttattgcct tttttggagg agctttccag cctgaattta cgggaagaaa
     2041 ccaatgacat ttccgccatg gaaaaaatgc cattgagcaa aaccaaacca ataatgatta
     2101 aaatttccat attgatgttg tgagtgacgg cattgaaacc gcccgacaaa gataaggcaa
     2161 tatattatac ctgaaaacgg tcattgctcg ctggccgaaa cggcacagta agttcaagta
     2221 ttaaactata tgcctgcact ttgtgtatat tagtcggtga gaatgagtgt gttggtctaa
     2281 gccggcattg ctcgttactc ccatccacat tacatttgcg gtcatgatta tagacctgtt
     2341 atttctcatc acggccatct atgggttgta tttaggatac tcccggggca tcatcaagac
     2401 ggtattcacg gttttgtcgg tgctgtttgg cgtcatcatt gcctttcgtt tttcgccgga
     2461 tatgacggaa ctgctggaat cactctttac ctctaccaat ccgctgatgt tcattgccgg
     2521 ctttctgctt actttcgtac tcacgatgat cgccatccgc acgtttgcgc gtgtattgga
     2581 aaatgggttg gaaagcgcca atatcaactt catcaatcag gcagcgggcg cggcgatcac
     2641 agcagctttt actaccctgt tgttcagtac tttagtgttg tttgccaaca aagcccacct
     2701 cattgaggaa gataccaagc aagattccat cacatattcc tatctggaaa aataccccca
     2761 gacgatgtgg ggtcttttgg gcaaagccaa gcccacttta cagcgatttt gggatcgctc
     2821 gttagagttc atggatcgtt tggaagacta ccgggtagaa gactccgaat ccgaacccac
     2881 tatctacgat attgaggagg acgaacccac ccgtaccact caataagccg gtcattcttt
     2941 ttcaggcaat ggggacgcaa agtgcagcgt acgttgcggg aacgggatgg agacgccgtg
     3001 ttctttaaac ttgcgccaga tagcaatgcg tatttcgctt ttcacattct ctattcgaaa
     3061 cacctcatcc gaccaa
//
LOCUS       DAORLF010000518         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17398_length_3072_cov_2.096122, whole genome shotgun sequence.
ACCESSION   DAORLF010000518 DAORLF010000000
VERSION     DAORLF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17398_length_3072_cov_2.096122"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>258
                     /locus_tag="PK198_25100"
     CDS             <1..>258
                     /locus_tag="PK198_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF42104.1"
                     /translation="TVAPASGKVLSGGVEANALQKPKKFFGAARKIEGGGSLTILATA
                     LIDTGSKMDGVIFEEFKGTGNMEIHLDRRMAEKRMYPAVNVN"
     assembly_gap    259..395
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            589..2760
                     /locus_tag="PK198_25105"
     CDS             589..2760
                     /locus_tag="PK198_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF42105.1"
                     /translation="MLPDILSYPAPLQKRIRQYWPDFIENYRRVYPQAGATDIQSLID
                     AVATAYKERSASLRKTDEARARREDWFQQPQQVGMMLYVHLFSGDLKKLAHHIDYFKE
                     LGVTYLHLMPLLQPRQGPNDGGYAVADYRNTDPRLGTMTDLRALAALLRKEGILLVVD
                     MVMNHTAREHAWAQAALRGEAHYQAYYWMFDDRSLPDAYERTLPEVFPDFAPGNFTWQ
                     PEIEKWVWTTFYNFQWDLNFSNPDVFAAMLGEMLFVANCGVDVLRLDAVPFLWKKMGT
                     NCQNQPEAVFMLAAYRALVRMAAPGVLFKSEAIVAPDDIIKYLGAGGLESKVCEIGYN
                     ATLMNHLWHALASENTHLLRTTLSQLPAIPAEATWLNYIRCHDDIGWGISDDNAAAVQ
                     QEGRGTRNFCTDFYTGALPGTYAEGYVFQRDRGTGEARVSGTAAALAGLQKAQVEAEP
                     KLIAAALRRLRLLNSIIFFMRGIPLIYSGDELGQLNDFSYLLDPERAGDNRWVHRPPM
                     NWPKAELRHSEGTVEQQLFSWHVHLAKVRAAQAALHGRSTDRILMPDNDHVFLCERMW
                     KGERLLLAANFSGSTQAVSLGVLPDAWRGEVCAELLSGRSLRFTDRELVLPPYGAFWL
                     QPAESFSARPNRRVKLSVHVRTEFGETVFISGSNEALGAWQLPAAVPAQANDYPWWTA
                     EVEMPEGEAFSYRWLRVRNREVVQYGPEIGRAVACWDGAES"
     gene            2826..>3072
                     /locus_tag="PK198_25110"
     CDS             2826..>3072
                     /locus_tag="PK198_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF42106.1"
                     /translation="MAFQKLPIGIQDFRTLIEDGYKYVDKTRYVHQLATTGKFFFLSR
                     PRRFGKSITIATLQELYSGSRELFKGLWIEDKWDWNKR"
BASE COUNT          656 a          823 c          805 g          651 t
ORIGIN      
        1 acagtagctc ctgcatccgg taaagtatta tccggcggtg tggaagccaa tgcgcttcag
       61 aagcccaaga agttcttcgg tgcagcccgc aaaattgaag gcggaggttc gctgaccatt
      121 ctggcaacgg cgctcattga tacaggttcc aaaatggacg gcgttatctt tgaagaattc
      181 aagggtaccg gcaacatgga gatccacctc gaccgtcgca tggccgagaa gcgcatgtac
      241 ccggcggtca acgtcaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccagc ggcaacgagg agtttctggc
      421 gtctatgaac ggctgattat tttaaaaata aagggcctgg cctcgaccag actgccggta
      481 ttgggaataa tgacccgata ccggcatttt ttttctgctg aaaacctact tttgcatccg
      541 gccgttagag cctgccgagc atatcgccaa tcaccacatc atcataccat gctacccgat
      601 atcctatcct atcccgcgcc gctgcaaaag cgcatccggc aatactggcc cgattttatt
      661 gaaaactaca ggcgcgtata tccgcaagcc ggcgctactg atattcaatc acttatagat
      721 gccgttgcca cagcttacaa agagcgctct gcttcattgc gcaaaacaga cgaagcgcgc
      781 gcccggcggg aagactggtt tcaacagcct cagcaggtgg gaatgatgct gtatgtgcat
      841 ctgttcagcg gcgatctcaa aaaattggcg caccatattg attatttcaa agaattaggc
      901 gtcacctatc ttcacctcat gcccttgctg caaccccggc aaggccccaa cgacggcggc
      961 tatgcggtgg ccgactatcg caacaccgac ccccgattgg gtaccatgac cgatctgcgc
     1021 gcattggcgg ctttgttgcg caaagaaggc atcctgttgg tagtggacat ggtgatgaac
     1081 cacactgccc gcgaacacgc ctgggcgcaa gcagcgcttc ggggggaggc tcattatcag
     1141 gcctactatt ggatgtttga cgaccgcagc ctccccgatg cctacgagcg cacgctgccg
     1201 gaggtatttc ccgacttcgc gcccggcaat ttcacctggc agcccgaaat agagaaatgg
     1261 gtatggacca ccttttacaa ttttcagtgg gatttgaatt tcagcaatcc cgatgttttt
     1321 gcggcgatgt tgggcgagat gctgttcgtt gccaattgcg gcgtggatgt gctgcgtttg
     1381 gatgccgtgc cttttttgtg gaaaaaaatg ggcaccaatt gccaaaacca accagaggca
     1441 gtgttcatgc tggcggctta ccgcgcattg gtgcgtatgg cggctcctgg cgtgctgttc
     1501 aaatcggaag ccatcgtagc gcccgacgac atcatcaaat acctcggcgc cggcgggctg
     1561 gagagcaaag tgtgcgaaat cggctacaac gctaccctga tgaaccactt gtggcacgct
     1621 ttggcttctg aaaatacaca tttgttgcgc accaccctct cacaattgcc ggccattcct
     1681 gccgaggcca cctggctcaa ttacatccgt tgccacgacg atattggttg gggcatttcc
     1741 gacgataacg ccgccgccgt gcaacaggag gggcgcggca cccgcaattt ttgcaccgat
     1801 ttttacaccg gcgcattgcc cggcacttac gcagaaggct atgtttttca acgcgaccga
     1861 ggcaccggag aggcgcgtgt atcgggaaca gccgccgcgc tcgccggcct ccagaaagcg
     1921 caagttgagg cggagcctaa actgatagcc gccgcccttc gcaggttgcg cctcctcaac
     1981 agcatcatct tttttatgcg cggcattccg ctcatctatt ccggcgacga actggggcaa
     2041 ctcaatgatt tctcttacct cctcgacccg gaacgcgccg gcgacaaccg ctgggtgcat
     2101 cgcccgccga tgaactggcc caaagccgaa ctgcgccact cagagggtac ggtggagcag
     2161 caactcttca gttggcacgt ccatctggca aaagtgcgcg ccgctcaggc agccctgcat
     2221 ggtcgttcga cagaccgcat cctgatgccc gacaacgacc atgttttttt gtgtgaaaga
     2281 atgtggaagg gcgaacggct cctattggcc gccaatttca gtggaagcac gcaggctgta
     2341 tctctgggcg ttttgcctga tgcctggcgc ggagaggttt gtgcagaatt gttgagcggt
     2401 cgttccctgc gattcaccga cagagaattg gtgttgcctc cttacggcgc tttttggctc
     2461 caacccgctg aatcattcag cgcgcgcccc aatcggaggg tgaaactgag cgtgcatgtg
     2521 cgcacggaat ttggagaaac ggtcttcatc agcggcagca acgaggcatt gggcgcctgg
     2581 caattaccgg ctgccgtacc cgcgcaggca aacgactatc cctggtggac tgccgaggtg
     2641 gaaatgcccg aaggcgaggc tttttcttac cgctggctgc gcgtccgcaa tcgggaagtg
     2701 gtgcaatacg ggccggaaat cgggcgggca gtagcctgtt gggacggagc ggagagctaa
     2761 agacagggga tatcgcaggc tccggcgtct gcattttttc gtatctttgc atacaaactg
     2821 cacaaatggc atttcagaaa ttacccatcg ggatacagga ttttcgcacc ctcatagaag
     2881 atggctacaa gtatgtggat aagacccgct atgtccatca acttgctacg actggaaaat
     2941 ttttcttcct ctcccgcccg cgccgttttg gcaagtccat caccatcgcc actttgcagg
     3001 agttgtattc cggcagccgc gagttgttca aaggcttgtg gatagaagac aaatgggact
     3061 ggaacaagcg gc
//
LOCUS       DAORLF010000519         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17417_length_3070_cov_2.134328, whole genome shotgun sequence.
ACCESSION   DAORLF010000519 DAORLF010000000
VERSION     DAORLF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17417_length_3070_cov_2.134328"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK198_25115"
     CDS             <1..228
                     /locus_tag="PK198_25115"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRF42107.1"
                     /translation="LYLDAGSGARNPVPARLIEAVRKSVRLPLMVGGGLRNPESVLGA
                     FNAGADIVVVGTAIEQEPGMLERMAEVVRRT"
     gene            326..1279
                     /gene="rsgA"
                     /locus_tag="PK198_25120"
     CDS             326..1279
                     /gene="rsgA"
                     /locus_tag="PK198_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRF42108.1"
                     /translation="MQTGIIIKSTGSWYQVLAQDGRRWQCRIVGKLRLEGIKLTNPVG
                     VGDEVQIVPENEAENTALIKGVQPRRNYVVRQSPRRKHDLHLLAANIDQAVVIVTIRE
                     PHLKQGFIDRFLLMTEPQDIPAVIIFNKADLYDEDDLALFGYLKNIYEAIGYGVMLTS
                     TADGTGIEAFRELLRDKVTLVGGQSGVGKSSLVNVIQPQLDLRTGDLSDYSGKGMHTT
                     TFAEMYALDFGGHLIDTPGIKTLAFNNLEQLDVAHNFREFFQMSDDCKFGGSCLHRSE
                     PGCAVRKAIESGALSELRYANYLALLDEVEDQNYWERHKDM"
     gene            1328..1852
                     /locus_tag="PK198_25125"
     CDS             1328..1852
                     /locus_tag="PK198_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42109.1"
                     /translation="MTLQEFFDMLGENPVYVLFFFFMLPFTALLAGWLGKGEGHISPW
                     KYLYSALIYLACVPGIFAVTLSIYFFLFERRSILDTNLYTQVLPIVSMLLTLFLIRKN
                     VNLDRIPGFDKIGGLITMISATLAIMWFIDRTHIYAITFLPFHVVLLIFAGLLLLIRF
                     GWSRFFGRSSSAGR"
     gene            complement(1849..>2338)
                     /locus_tag="PK198_25130"
     CDS             complement(1849..>2338)
                     /locus_tag="PK198_25130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42110.1"
                     /translation="SEGTRGFISEDGFYKVRVEVELPLYGRASGFVAQDTFDINLGDL
                     NEVDFAEFKLFTKNELPLAVELQGYFLDENNQILDSLFQTQQLVVQGAPVNGQGISTG
                     VSTKSTLIPFSEERLARIKSARRILVNTAFSTTNGGANSIKVSANQKIQVQLGAKLGK
                     KR"
     assembly_gap    2339..2431
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2432..>3070)
                     /locus_tag="PK198_25135"
     CDS             complement(<2432..>3070)
                     /locus_tag="PK198_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42111.1"
                     /translation="SSPGGLALDRIDCKTGTFTYYVTNRHPESVNATITFPDVKKNGQ
                     PLRFQMTLPPYSGTGDAPFSTNIFSPASVEGYIIESDIDSVFIEYEAIRASGISDTLA
                     SVLVSIQNLTFSYAEGSLSNYQFAGNRDTIEIDFFDNWTRGDVYFEDPTVTFHIENSF
                     GIPTRSLVRVFDIITVNGEVLPLQSPHITNGIDFPYPSINEVGQVKTRSFVFN"
BASE COUNT          705 a          740 c          788 g          744 t
ORIGIN      
        1 ctgtacttag atgcaggaag cggcgcccga aatcccgtgc cggcccggtt gattgaggcc
       61 gtgagaaagt cggtgcgcct gccgctgatg gtaggaggcg gcttgcgcaa cccggaatct
      121 gtgctgggcg cattcaatgc cggagccgat attgtagtgg taggaacagc catagaacaa
      181 gagccgggta tgttggagcg catggcggaa gtggtgcgcc ggacttaaat ttctatatgt
      241 agtgaagggg tgactacgag ctgtgttctt ggctgagtca ccccttcact acactaattg
      301 atatgcaaat acaaaaacga agactttgca aacaggaatc atcataaagt caacaggcag
      361 ttggtatcag gtgctcgctc aggatgggcg ccggtggcaa tgtcgcattg tgggaaaact
      421 gcgcttggaa ggcatcaagc tcaccaaccc cgtaggcgtg ggtgacgagg tgcaaatagt
      481 gcccgaaaac gaagctgaaa acaccgcgct catcaaaggc gtgcagcccc gccgcaatta
      541 tgtggtgagg caaagccccc gccgcaagca cgatctgcac ctgctggcgg ccaacattga
      601 tcaggccgtc gttatcgtca ccatccgaga gccgcacctc aagcagggct tcattgatcg
      661 ctttttgctg atgaccgaac ctcaggacat tcccgccgtc atcattttca acaaggccga
      721 cctgtacgat gaagacgatc tggcgctgtt tggatacctc aaaaacattt acgaggccat
      781 cggctacggc gtcatgctca cttcgacagc cgacggcacg ggcatagagg cgttccggga
      841 actactgcgc gataaggtta cactggtagg tgggcagtcc ggcgtgggaa agtccagctt
      901 ggtcaacgtc attcagccgc aattggacct gcgcaccggc gacctctccg attacagcgg
      961 caagggtatg cataccacga cttttgcgga aatgtacgcg ctcgattttg gcggccacct
     1021 gattgatacg ccaggcatta aaacgctggc attcaataat ctggagcagt tggatgtggc
     1081 gcataatttc cgggagtttt ttcaaatgtc ggatgattgc aaatttggcg gctcctgcct
     1141 gcaccgttcc gaaccgggct gcgccgtgcg gaaagccatt gaaagcggtg cgttgagcga
     1201 gttgcgctac gccaattatt tagccttatt ggatgaagta gaggatcaga actactggga
     1261 gcggcacaag gatatgtgaa aatcttgtac cttgcgttcg cttttttgtg gcaaaaatca
     1321 tactgatatg accttgcagg aattcttcga tatgctgggc gaaaacccgg tttatgtgct
     1381 cttctttttc tttatgcttc cgttcactgc cttgctggca ggctggttgg gcaaaggcga
     1441 gggtcatatt tccccgtgga agtatttgta ctccgcgctg atttatctgg catgtgtgcc
     1501 gggcattttt gcggtcacac tcagcattta cttttttctc tttgaacgcc gcagcattct
     1561 cgataccaac ctttatacgc aggtgttgcc catcgtttct atgttgctca ccttgttcct
     1621 gattcgcaaa aacgtgaact tggaccgcat tcccgggttc gacaaaatag gcggcctgat
     1681 caccatgatt tccgccacct tagccatcat gtggttcata gatcgcaccc acatctacgc
     1741 catcaccttc ctgcccttcc atgtagtgct gctcattttt gcaggcttgt tgctgctcat
     1801 ccgttttgga tggtcgcggt tctttggtag gtcgtcttcg gccggtcgtt aacgcttctt
     1861 acccaatttg gcccccagtt gaacctgaat tttctggttt gccgatactt tgatggaatt
     1921 ggcgcctccg ttggtggtag aaaaggccgt gttgaccaat atcctgcggg ccgatttgat
     1981 gcgggccaag cgttcctccg agaagggaat gagcgtggat ttggtggaaa cgccggttga
     2041 aatcccctgt ccattgaccg gagcaccctg aacaacaagc tgttgcgtct ggaaaagtga
     2101 atcgagtatt tggttgttct catccaaaaa atagccctgt aactctaccg ccaagggcaa
     2161 ttcgtttttg gtaaacagct tgaactcggc aaagtccacc tcgttcaaat cccccaagtt
     2221 gatgtcaaaa gtatcctgcg ctacaaagcc ggaagcccgg ccgtacaaag gcagctctac
     2281 ctctacacgc actttgtaaa agccatcttc actgataaag ccccgcgttc cttcacttnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattaaaaac aaaacttctt gttttcacct
     2461 gccctacctc gttgatggag ggatatggaa aatctatgcc gttggtgatg tgcgggcttt
     2521 gcaacggaag cacttcgcca ttgacggtaa tgatatcaaa aacacgaacc agagaacgcg
     2581 tcggaatacc gaaggagttt tcgatgtgaa atgtaaccgt agggtcttca aaatacacat
     2641 cgccgcgcgt ccagttgtca aaaaagtcta tttctatcgt atctcggttg ccggcaaact
     2701 gatagttgga aaggctcccc tcggcatagc tgaatgtaag attttggata gacaccagga
     2761 cgctggccaa agtatcgctg atgccggaag cgcgtatggc ttcatattcg ataaaaacag
     2821 agtcaatatc cgactcaatg atatagcctt ccaccgaggc cggagaaaaa atattagtag
     2881 agaacggagc atctccggta ccgctgtagg gaggcagtgt catctggaat cgcaggggct
     2941 ggccgttctt ttttacatcg ggaaaggtga tggtggcatt aacgctttcc gggtggcgat
     3001 tggtcacata ataggtgaat gtgccggttt tgcagtcaat gcgatccaag gccaaaccgc
     3061 ctggggaaga
//
LOCUS       DAORLF010000520         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17507_length_3058_cov_2.394605, whole genome shotgun sequence.
ACCESSION   DAORLF010000520 DAORLF010000000
VERSION     DAORLF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_3058_cov_2.394605"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PK198_25140"
     CDS             <1..1087
                     /locus_tag="PK198_25140"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HRF42112.1"
                     /translation="GCLGRSSCVVTTLGPLTHSPITTHTLTTTMTHTFSCIDAHTCGN
                     PVRLVSGGAPFLKGNSMFERRQYFLEHHDWIRKGLMFEPRGHDMMSGSMLYPPVDEAN
                     DVGVLFIETSGCLPMCGHGLIGTVTVAIEHGLVRPQTPGKLNVETPAGLVRVSYRQEG
                     GKVLSVKLVNVPSFVILSDAHIDTQCLGRLYFDVSYGGNFYAIMDEQENFKGIQHYTA
                     SELIAFSREIRVLINEKYQVAHPENPAINYVSHVLWAGSPLHEGSTARNAVFYGEKAI
                     DRSPCGTGTSARLAQWVQRGRLGIGEDFVHESIIGSRFTGRAEETVQVGPYPGIVPSI
                     EGWARVTGINTIFIEDDCPFREGFQVI"
     gene            1247..2500
                     /locus_tag="PK198_25145"
     CDS             1247..2500
                     /locus_tag="PK198_25145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF42113.1"
                     /translation="MAKIGIIGGGINGLMAAYYLQKDGHEVTVLDRGDFSEGCSHGNA
                     GMIVPSHIIPLAAPGVVMKGLKWLLNKKSPFAIVPSWDKDLIYWLFAFHRAATETNVQ
                     RAIPQLAALSLLSKKLFQEMAAEGEIGLNLQEKGILMLCQTAATEHEEHHVARLANAQ
                     GIQAVPMTAAELAALNPQVRYSVRSAVHYPGDATIDPALALASLTAHLKSRGVVFLPN
                     TEVTGFRRGKDRISALQCADGERNYDHYVLCGGAWSGMLMKSVGLRLPVTGGKGYSFV
                     QNNAAGLSIAALLLDHRVSVTPYGQTVRFGGTMELGPLNSRLNMAKVSGIHSAIGQYF
                     ADWKSPEPTPQQVWHGFRPCSPDGLPYLGTVQMYGNLHIAAGHGMMGVSLAPASGKII
                     ADLVQGRTPDLEDMSAFRPERFASR"
     gene            complement(2506..>3058)
                     /locus_tag="PK198_25150"
     CDS             complement(2506..>3058)
                     /locus_tag="PK198_25150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42114.1"
                     /translation="IWSKGLGFLVSGGMKYAVLFLFMVVIYHISNRAMDALTGQPSHI
                     SFKDFIDSQKRALGLTITALVLESITILILKIFFGIFDFISPIQPVLVFAVKAYWVGF
                     VVVDSYQEQFGFTIKQSNRFMTRYIGVGLAIGVVFKVLMFIPVFGTAIASFVTSVTAV
                     VVMHELAKLHLAEPGQMEWEKSA"
BASE COUNT          697 a          855 c          893 g          613 t
ORIGIN      
        1 cgggtgcctc ggtcgctcct cctgcgtcgt cactaccctc ggccccctca cgcactcacc
       61 aatcaccact cacacactca ccactaccat gacacacacc ttctcctgca tagacgccca
      121 cacctgcggc aaccccgtgc gcttggtcag cggaggcgcg ccctttctga aaggcaactc
      181 catgttcgag cggcggcagt actttttgga gcaccacgac tggatacgca agggcctcat
      241 gttcgagccg cgcggccacg atatgatgtc gggcagcatg ttgtatcccc ccgtggacga
      301 ggccaacgac gtgggcgtgc tcttcatcga gacctccggc tgcctgccca tgtgcggtca
      361 tggcctcatc ggcacggtga cggtagccat cgagcatggc ttggtgaggc cccaaacacc
      421 gggcaagctg aacgtggaaa cgccggcggg cttggtgcgc gtcagctacc ggcaggaagg
      481 cggcaaagtg ctgtcggtca agcttgtcaa cgtgccgtct tttgtcattc tcagcgatgc
      541 gcacattgat acgcaatgcc tggggcgcct ctatttcgat gtctcttacg gcggcaattt
      601 ttacgccatc atggacgagc aggaaaactt caaaggcata cagcactaca cggcctccga
      661 actgatcgcc ttcagccgcg aaattcgggt cttgatcaac gaaaaatatc aggtggcgca
      721 ccctgaaaat ccggccatca actatgtgtc gcacgttttg tgggccggaa gtccgctgca
      781 cgagggttct acggcgcgca acgcggtgtt ttacggcgaa aaagccattg atcgctcgcc
      841 ttgcggtacg ggcacctcgg cgcgcctggc gcagtgggtg cagcgcgggc gcttgggcat
      901 cggcgaggat tttgtacacg agagcatcat cggcagcagg ttcacgggcc gggcagagga
      961 gacggtgcag gtggggccgt atccgggcat cgtgccgagc atagagggct gggcgcgggt
     1021 gacgggcatc aacaccatat ttatagaaga tgattgcccg ttccgggagg ggtttcaggt
     1081 gatttaacga taggcaatga cagttggcct caagccctta gctgcaagcc gcgagttttt
     1141 taacgcgaat gttagcgaag ggcgcgaatt tccgcgaaag cgtagcttac agctaaaagc
     1201 ctgcagccgt tcctcccgga acccgaaaca ttaaacgtta aaaaccatgg caaaaatcgg
     1261 catcataggc gggggcatca acggactgat ggccgcgtat tacctgcaaa aagacggaca
     1321 cgaagtgacg gtattggatc gcggcgattt cagcgaaggc tgttcgcatg gcaatgccgg
     1381 catgatcgtg cccagccaca tcataccatt agcagcgccc ggcgtggtga tgaagggatt
     1441 gaaatggctt ttgaacaaaa aaagcccctt cgccatcgta ccctcatggg ataaagactt
     1501 gatttactgg ctgtttgcat tccatcgggc agctacggag accaatgtgc aacgagccat
     1561 tccgcaattg gcggcgttgt ctttgctgag caaaaagtta tttcaggaaa tggccgccga
     1621 gggcgaaatc ggcctcaact tgcaggaaaa aggcatcctc atgctgtgcc aaaccgccgc
     1681 taccgagcac gaagaacacc atgtcgcccg cttggccaat gcacagggca tacaggctgt
     1741 accgatgacg gccgccgaac tggccgcact caatcctcag gtgcgctact cggtgcgcag
     1801 cgcggtgcat taccccggcg atgccaccat tgaccccgcc ttagcgctgg cctctttgac
     1861 ggcgcacctg aaaagccggg gcgtagtttt tttgccaaat acagaggtca ccggttttcg
     1921 gaggggcaaa gaccgcatca gcgccttaca atgcgccgat ggagagcgca actacgatca
     1981 ctatgtattg tgcggcggcg cctggtcggg catgttgatg aaaagtgtgg gacttcggtt
     2041 gccggtaacg ggcggcaagg gctacagttt tgtgcaaaac aatgcggccg gactgagcat
     2101 cgcggcgctc ctgctcgatc accgggtgag cgtgacgcct tacgggcaaa cggtgcgttt
     2161 tggcggcacg atggaactgg ggcctctcaa ttcccggctc aacatggcca aagtgagcgg
     2221 catacacagc gccatcgggc agtattttgc cgactggaaa tcgccggaac cgacgccgca
     2281 gcaagtctgg cacgggttca ggccctgctc tcccgacggg ctaccgtatt tgggcaccgt
     2341 tcagatgtat gggaacctgc acatcgcagc ggggcacggc atgatgggcg tgagcctggc
     2401 gcccgcgagc gggaaaatca tagccgatct ggtacagggc agaacgccgg atttggagga
     2461 tatgagcgcg ttcaggccgg agcgatttgc ttcaagatga tgttttcaag cgctcttttc
     2521 ccactccatc tgccccggtt ctgcgaggtg cagtttggcc aattcgtgca tcaccaccac
     2581 cgccgtgacc gaggtgacaa atgaagcgat ggccgtgccg aatacaggaa tgaacatcag
     2641 cactttgaat accacaccaa tggctaagcc cacaccgatg tagcgggtca taaaccggtt
     2701 gctttgtttg atggtgaacc cgaactgttc ctgatagctg tccaccacca caaaacctac
     2761 ccagtatgcc ttgacggcaa aaacgagtac cggctgaatg gggctgatga agtcgaagat
     2821 gccgaagaaa atcttgagta tcaggatggt aatggattcg agcactaaag ccgtgatggt
     2881 gaggcccaga gcgcgtttct gggagtctat aaaatctttg aaactgatgt gcgaaggctg
     2941 accggtgagc gcatccatgg cccggttgct gatgtggtaa atgacgacca tgaacaaaaa
     3001 cagcaccgcg tacttcatgc ccccgctgac gagaaagcct aaacctttgg accatata
//
LOCUS       DAORLF010000521         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17539_length_3055_cov_1.874000, whole genome shotgun sequence.
ACCESSION   DAORLF010000521 DAORLF010000000
VERSION     DAORLF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17539_length_3055_cov_1.874000"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK198_25155"
     CDS             <1..382
                     /locus_tag="PK198_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRF42115.1"
                     /translation="IDIFLKKEREHVSVGHNALAGFIYFLTFIAFLVQCITGFGLYAS
                     MSTWWFADLFVWAGHLFGGDFALRNIHHAAMWFFILFTIVHVYLVFYHDYVEGRGEIS
                     SMGGGWKFIEEDSFKKQREEDQKS"
     gene            382..>578
                     /locus_tag="PK198_25160"
     CDS             382..>578
                     /locus_tag="PK198_25160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRF42116.1"
                     /translation="METETRGRLLILGLGNLLMGDEGLGVHFAQRLEQRNDLPAGVDI
                     LEGGTSGFLLTEYFVGYPTMIL"
     assembly_gap    579..615
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <616..866
                     /locus_tag="PK198_25165"
     CDS             <616..866
                     /locus_tag="PK198_25165"
                     /inference="COORDINATES: protein motif:HMM:NF013877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRF42117.1"
                     /translation="LIHPRFSSDFPKSLSTHDIGLKDLLDGLTLLGRMPEIHLFVVSI
                     QDLANMEIGLSPEVEAVLPELERQVLELAQSLVSKVDA"
     gene            996..1595
                     /locus_tag="PK198_25170"
     CDS             996..1595
                     /locus_tag="PK198_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRF42118.1"
                     /translation="MVPQFLLDIKDFISQPWPWYVAGGMITLIMFLLLWLGKDFGMSA
                     NLRVMCAADGAGDYSDFFKFDWQSQGWNLMVALGAMFGGYIAAHYFSTPGEEVAHVSA
                     ATVESLRNLSPDLNFAEGAVPLIPDFYSWEALFSLRGILVIVVGGFLVGFGARYAGGC
                     TSGHAISGLSALQFSSLVAVIGFFLGGLVMTHFILPLIF"
     gene            1609..2031
                     /locus_tag="PK198_25175"
     CDS             1609..2031
                     /locus_tag="PK198_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRF42119.1"
                     /translation="MRLSKYIFIGILLGLTLYKSEAVSWFRIYEMFHFQSFHMYGIIG
                     TSVIAGILVVQLIKRTHLKSIEGKEIVIQPKDKAYARYILGGFIFGLGWALAGVCPAP
                     MFILMGSGYTVFIVFLAAAMLGTFFYGVARKYLPHNMK"
     gene            2430..3029
                     /locus_tag="PK198_25180"
     CDS             2430..3029
                     /locus_tag="PK198_25180"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRF42120.1"
                     /translation="MTALLLIDFQNDFFPFGTIEAKNADTALVQIVNDLMPRFDHVIA
                     LCDEHPADHNSFAANHLWRRPWQTMNTDAGERLLWPMHSVAGSFGADWAAGLHHEKIE
                     RTFAKGLHPDATGYSGFENPTLESWLHAAGVQQLYLVGTLLEYSVRHTALNAMQRGFA
                     TTVLLQGCGFLELHPGDSEQAIAEMQQAGVLVTRSAPVF"
BASE COUNT          630 a          871 c          760 g          757 t
ORIGIN      
        1 aattgacata ttcctgaaaa aggaaagaga gcacgtgtcg gtcggtcaca acgcattggc
       61 aggcttcatc tactttctta ccttcatcgc ctttttggtg cagtgcatca ccggttttgg
      121 gctgtacgcc tccatgagta cctggtggtt tgccgatctg tttgtgtggg cgggccatct
      181 cttcggcggc gatttcgctt tgcgcaacat ccaccatgcg gccatgtggt tctttatttt
      241 attcaccatc gttcacgttt acctcgtttt ttaccacgac tatgtggaag ggcgcggcga
      301 aatttcctct atgggcggcg gctggaaatt cattgaggaa gattcattta agaaacaaag
      361 agaggaagat caaaaatcct gatggaaaca gaaacgcggg gcaggctgct catcctcgga
      421 ttgggcaacc tgctgatggg agatgaagga ttgggcgtgc attttgccca gcggttggaa
      481 caacgcaatg acctgcctgc aggcgttgac atattggaag gcggcacctc cggctttctg
      541 cttaccgagt attttgtagg atacccgacc atgatactnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnggctg attcatcccc gcttctcctc agatttcccc aaatcgctca
      661 gcacccacga catcggcctc aaagacctcc tcgacgggct gaccctgctg gggcgcatgc
      721 ccgaaattca cttgtttgta gtctccattc aagaccttgc caacatggaa atcggcctct
      781 cgcccgaagt agaagccgtg ctgccggaat tggagcgcca ggtgctggag cttgcgcaat
      841 cccttgtttc taaggtggat gcgtagtttg ttttttagaa atctgccttt tcccaaccta
      901 tattcgtacc ataaccttat ttttgaccaa cagcttgagc cgcgctgagg cgcggttgaa
      961 gccgaccttt caacgtacaa tcaaaaacac ttcacatggt acctcaattt ctattagaca
     1021 tcaaagactt catcagtcag ccctggcctt ggtatgtggc gggcggcatg attacgctca
     1081 tcatgtttct gctgctgtgg ttgggcaaag acttcggcat gtcggccaat ctccgcgtca
     1141 tgtgtgccgc cgacggcgca ggcgattatt ccgatttttt caaattcgac tggcaatcac
     1201 agggttggaa cctcatggtg gcgctcggcg ccatgttcgg cggctacatt gcggcgcact
     1261 acttctccac gcccggcgag gaagtggcgc atgtttctgc cgctacggtc gaatcgcttc
     1321 gcaatctcag ccctgatctc aatttcgcag aaggcgccgt gccgctcatc cccgacttct
     1381 actcctggga ggcgctcttc agtctgcgcg gcattttggt tatcgtcgtc ggcggttttc
     1441 tggtaggctt cggcgcgcgt tatgccggcg gttgcacatc ggggcacgct atcagcggct
     1501 tgtcggccct ccagttttca tcattggtag ccgtcatcgg gttcttcctc ggcggcttgg
     1561 tgatgacgca tttcatcctt ccactcattt tctaaaacac tcattgccat gcgcttatcc
     1621 aaatatattt tcatcggcat cctgctcggc ctcacgctat acaagtccga agccgtatct
     1681 tggtttcgca tctacgaaat gttccatttt cagtccttcc acatgtacgg catcatcggc
     1741 acgtccgtca tagccggcat tctggtggtg caactcatca aacgcaccca cctgaaatca
     1801 atagagggca aagaaatcgt cattcagccc aaagacaaag cttatgcccg ctacatcctc
     1861 ggcggcttca ttttcggctt gggctgggcg ctggccggcg tatgtcctgc ccccatgttc
     1921 atcctcatgg gcagcggcta cacggtgttc attgtgtttc tggcagccgc catgttgggt
     1981 acgtttttct acggcgtggc cagaaagtac ctgccgcaca atatgaagtg agttgtttgg
     2041 ggggcagccg cccgctccat ctccagagct gttagcttta gctccgcagt atcccgccaa
     2101 agctaacagc tctcatcctg ctgcgggcgc cgggccatcc gctttacctc ctaaggtcgg
     2161 tatcgctcct gtccctgccg caattcaggg gtgagtgggt gaggagtgaa caacccgaaa
     2221 ccttgaatct ggaacccgtg ggcggtcggc agtaggcggt aggcggtgct aagcttggct
     2281 tgcagcttgt agccagcagc ttgtggccag cagccagcag ccgtttctac ccgaaacctt
     2341 aaacccgaaa tcaacccgtt cctaccagca aaatccgaaa tcagaaatta acctgttgct
     2401 atcagaaaat ccgaaatccg aaatttctca tgaccgcact cttactcatt gattttcaga
     2461 atgatttttt ccccttcggc accatagagg caaaaaacgc cgacacagca ctcgtgcaga
     2521 ttgtcaacga cctcatgccc cgcttcgatc acgtcatcgc cctctgcgac gaacaccccg
     2581 ccgatcacaa ctccttcgca gccaatcacc tgtggcgccg cccttggcaa accatgaaca
     2641 cagacgccgg cgagcgcctc ctctggccca tgcacagtgt ggccggcagt ttcggcgcag
     2701 actgggccgc aggattacat cacgaaaaga tagagcgcac ttttgccaag ggcctccacc
     2761 ctgacgcgac cggctacagc ggatttgaaa atccgacgct ggagtcatgg ctgcacgctg
     2821 ccggtgttca gcaattgtac ctcgtcggca cactcttgga gtacagcgtc cggcacacag
     2881 ccctcaacgc gatgcaacgg ggctttgcca caacggtact cctgcaaggc tgcggctttt
     2941 tggaactcca tcccggagat tccgaacagg ccatcgccga aatgcaacag gccggggttc
     3001 ttgtaacccg aagcgcgcct gttttttgac gctgatcttt atgatttata tgatt
//
LOCUS       DAORLF010000522         3033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17711_length_3033_cov_2.370719, whole genome shotgun sequence.
ACCESSION   DAORLF010000522 DAORLF010000000
VERSION     DAORLF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17711_length_3033_cov_2.370719"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2846
                     /locus_tag="PK198_25185"
     CDS             <1..2846
                     /locus_tag="PK198_25185"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF42121.1"
                     /translation="PTASFSAAPSPVCIGQTSTLTFSGNAGGTAAFAWNFDGGTATPG
                     TGQGPHSVTWATAGSKTISLTTTENGCASTPVNQTVVVEAPLAAPVINCATSTSQIVF
                     SWADVPGATGYAVTVLAGPMGVQSGNTYTVSGLMPGDASEISVTALSNGPCGPSSATL
                     SCVAQACPTVNIAIAPVAPICLTSVTTPITLSATVTGGGGAGTRTWAGPGITNAATGV
                     FTPATAGPGMHTISLTYQEGNCSFNASMTITVVAQPVAGFTAPAAVCIGAAATLSFSG
                     TAPVGADYTWNFDGGTATPGTGQGPHDVTWATAGSKTVSLTVGINNCLSAPFQQTIQV
                     DAPLAAPQITCSGTTTSITFSWAVVPGATGYQAVSTSGPQGVLSGTTYTVSGLSPNTS
                     VTVQVTALSNGPCGNSSAEATCIAQNCPTLTLSLTGTQAICAGDPAQVTLNFAGASAG
                     PFTVRYSINGGTPQTGVFTNGQSIPVPAIATTTITAISITDNSLPDCVYPSSATWTIT
                     VNQPVSAGTPTAAARLCAGVGTTLILSDLLENETPGGTWSETSALPSSGGAFNAASGT
                     FTASGQAARTYTFRYNVQGAAPCPAQQSTVTVILEPSPQADAGTDQTLTCNMGMVSIG
                     GSGTPVGQDITYLWSSATPGVTIADPTARIIEVAQPGTYVLTVRNDIGCTATDEVVVG
                     ADFAAPTAEVEVSPISCFQAGDGALNITRVNGGQAPYLYSLDGGAFGAQQFFTGLGPG
                     RHTLVVRDHNGCFSELSFVLDNPQQLIVNIRTNLSGTGNLIQRGDSVQLEAVVNGGAA
                     IDSIFWKPDSISQNAVLIRVQPRIATTYSVTVVDANGCRADDQITIYVEHDRRVFIPN
                     AFSPNDDGVNDVFSIFTDNSQVANVRSFFVFNRWGEAMFELYNFLPNNPSLGWNGKHR
                     GKAMNSGVYVYVAEIEFTDGEVIIYKGDVVLTQ"
BASE COUNT          651 a          921 c          820 g          641 t
ORIGIN      
        1 ccccgacggc ctcgttcagt gccgcgcctt ctccggtttg tatcgggcaa acctccacgc
       61 tcaccttttc aggtaatgca ggaggaacgg ccgcttttgc atggaatttc gacggcggaa
      121 cggccacacc cggaaccgga caaggcccgc attcggtgac ctgggccact gccggcagca
      181 aaaccatctc gctcaccacc accgaaaacg gatgtgcttc cacgcctgtg aatcaaacag
      241 ttgtagtgga agctccattg gccgcacctg ttatcaactg cgctacctcc acttctcaaa
      301 tcgtcttctc ctgggccgat gtgccgggcg ctacgggcta cgccgtcacc gtattggccg
      361 gtccgatggg cgtacaaagc ggcaacacct acaccgtaag cggcctcatg cccggcgatg
      421 cctctgaaat cagcgtgacc gcgctgagca acgggccttg cgggccgagc agcgcaacgt
      481 tgagttgcgt ggcgcaagct tgtcctaccg tcaatattgc catagcccct gtggcgccga
      541 tatgcctcac ctctgtcact acgcccatca cgctgtcggc taccgtaact ggcggcggag
      601 gcgcaggcac gcgcacctgg gccgggccgg gcatcaccaa tgccgcaacg ggcgtgttca
      661 cccccgctac cgccggaccg ggcatgcaca ccatttcgct cacttatcag gaaggcaatt
      721 gcagcttcaa tgcctctatg acgataacgg tcgttgcaca gccggtggcc ggctttacgg
      781 ctcctgcggc agtttgcatc ggcgctgccg caacgctgag cttcagcgga acggcgcctg
      841 tgggagctga ttacacctgg aatttcgacg gcggaacggc tacccccgga accggacaag
      901 gcccacatga tgtgacttgg gctacagccg gcagcaaaac cgtatcgctc accgtaggca
      961 tcaacaactg tttgtcggcg cctttccaac aaaccataca agtagatgcg ccattggccg
     1021 cgccacaaat cacctgtagc ggcaccacta catccatcac cttctcttgg gctgttgttc
     1081 ccggcgccac ggggtatcag gctgtgagta ccagcgggcc gcagggcgtt ttgagcggaa
     1141 ccacttatac cgtgagcggg ctttcgccca atacctctgt gacggttcag gtgacggcgc
     1201 tgagcaatgg gccttgtggc aatagttctg ccgaagctac ctgtattgct caaaactgcc
     1261 ccactttgac cctcagcctc accggcactc aggccatttg tgcaggcgat ccggcgcaag
     1321 taaccttaaa ttttgcagga gcaagcgccg ggccgttcac cgtgaggtac agcatcaacg
     1381 gcggcacgcc tcaaaccggc gttttcacca atgggcaaag cattccggtg ccggcaatcg
     1441 ctactactac catcaccgcc atttctatta ccgacaattc cctgccggac tgtgtgtacc
     1501 cgtcttcggc cacttggacg atcaccgtga accaacccgt aagcgccggt acgcccacag
     1561 ccgctgcaag gctctgcgca ggcgtgggta ctacactcat tctgagcgat ttattagaaa
     1621 atgaaacgcc cggcggcact tggtctgaaa cctccgcatt gccttcttcc ggcggggcat
     1681 tcaacgcagc gtcaggcact ttcaccgcat cagggcaagc ggcccgcacc tacactttcc
     1741 gatacaacgt acagggcgca gcgccttgcc cggctcaaca atctacggtg accgtcattc
     1801 tggaaccctc tccgcaagcc gatgccggca ccgatcaaac cctgacctgc aacatgggca
     1861 tggtgagcat aggcggttcg ggaacgcctg ttggtcagga cattacctat ctttggagca
     1921 gcgcaacacc gggcgttacc attgccgatc ccacggcccg catcatcgag gtggcacaac
     1981 ccggcactta tgtactcacc gttcgcaacg acattggttg caccgcgacg gacgaggtgg
     2041 tggtcggcgc tgattttgca gcccccacag cagaggtaga ggtctccccg atttcctgtt
     2101 ttcaggccgg cgacggggcg ctcaacatta cccgagtgaa cggagggcag gcaccctacc
     2161 tgtattcctt agacggcgga gctttcggcg cgcagcagtt tttcacaggt ttaggccccg
     2221 gacggcatac tttggtcgtg cgcgaccaca acggctgttt ttcagaattg agttttgtac
     2281 ttgacaatcc gcagcaactg atcgtaaaca ttcgcaccaa tctgagcgga acgggcaatc
     2341 tgatacagcg cggcgacagc gtgcagttgg aggcagtcgt gaatggcgga gcagccatag
     2401 acagcatatt ctggaagccg gattcgattt ctcaaaacgc tgtcctcatt cgggtgcaac
     2461 cgcgcattgc cactacttac agcgtaacgg tggtggacgc caacggctgc cgggccgacg
     2521 atcagatcac catttatgtg gagcacgatc gcagggtatt cattcccaat gccttctcgc
     2581 ccaacgacga cggggtgaac gatgtgttta gcatttttac cgacaacagt caggtggcga
     2641 atgtacgctc gttcttcgtg ttcaatcgct ggggcgaagc catgtttgag ttgtacaatt
     2701 tcctgcccaa taacccttct ctgggttgga acggcaagca ccgcggcaag gccatgaaca
     2761 gcggcgtgta tgtttatgtg gccgagatag aattcaccga tggcgaagtg attatttata
     2821 aaggagatgt ggtgttgacg cagtaagcgc tccagccgct accatcggag accgcagcaa
     2881 gccggaaagc ccaagagctt tgctttccgg cttgctttgt agaaaagcgt cttcccgtat
     2941 tttcacaagc acctcatttt aaagcattca cattctttgt agtggggcaa aaataaagtg
     3001 ttcattttct ttaggccaaa ggattagata aga
//
LOCUS       DAORLF010000523         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17748_length_3027_cov_2.280283, whole genome shotgun sequence.
ACCESSION   DAORLF010000523 DAORLF010000000
VERSION     DAORLF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17748_length_3027_cov_2.280283"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..558
                     /locus_tag="PK198_25190"
     CDS             154..558
                     /locus_tag="PK198_25190"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF42122.1"
                     /translation="MHKSLLLFIVLMLTTIGAMAQGKVTVKEDPVITQMMDRYTQTNK
                     ARRTVDGWRVQIMATPDRQNLDRSLQSFQYRYPNLSADWVHNNPYYKIRVGAFSTRLE
                     AQRLLNILKPDYPGAYLVADNQMKPAELLGTY"
     gene            565..1971
                     /gene="rodA"
                     /locus_tag="PK198_25195"
     CDS             565..1971
                     /gene="rodA"
                     /locus_tag="PK198_25195"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRF42123.1"
                     /translation="MPTSSRHGWRNIDWVAFSIFLSLVAIGWLMVYTVNYPELERGVN
                     FFSSTAGKQTVWLFIAMMVFAVVMVIDWKFWQTFNLPIYVGGILLLIGVLFLGSNIKG
                     ATSWFAFGGFSFQPSELAKVGTALALAGYLSRYGTNLRFARSQAIVLGIIIIPMILIL
                     WQPDAGSALTFLSFFFLLYREGLSPNIYAVGFFLAGVALLSLLFNPILIPILITLGGF
                     YVLAGNFREPKHWRRAALAASVLAAVAVLGFKQYWIALGLGLTGFLVAGFLHLARYNA
                     RLAGMLTAVLVVGSGLAFSTDYAFNNIMKPHQQDRINVWLHPERCDPRGSLYNVLQSK
                     MAIASGGLEGKGFLEGAMTKMNYVPEQSTDFIFCTVGEEQGFIGSMTVIVLFLAFLLR
                     LIFIAERQRSPFSRHFAYGVAGLLFVHFFVNIGMTMGLLPIIGIPLPLISKGGSSLLG
                     FTIMIGILLKLDSNRYSL"
     gene            1983..>3027
                     /gene="tgt"
                     /locus_tag="PK198_25200"
     CDS             1983..>3027
                     /gene="tgt"
                     /locus_tag="PK198_25200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRF42124.1"
                     /translation="MFSFTLQHTDPHSKARAGLIQTAHGEIATPIFMPVGTVGTVKAV
                     HLHELRDEIEAQIILGNTYHLYLRPGLDIIRGAGGLHRFIGWERPMLTDSGGYQVYSL
                     SGTRKIAEQGVTFSSHIDGSKHVFTPEGVMDIQRVIGADIIMAFDECPPYPSDYKYAR
                     NSMEMTHRWLQRCIQRFDETEGLYGYEQTLFPIVQGSTYEDLRTASAETIAAAGRPGN
                     AIGGLSVGEPEEEMYRFTELVCNILPKDKPRYLMGVGTPVNILEGIALGVDMFDCVMP
                     TRNARNGWLFTRYGDVKIKNARHKTDHQPLDATCTCYACAGKAGVNWADGGRDGFSRA
                     YLHHLDRCGEMLGP"
BASE COUNT          615 a          869 c          840 g          703 t
ORIGIN      
        1 tttggttttt ggaaggtaaa atagagcgat gaagtcgccc agtgttggag aagagataac
       61 agactcatgg ctcgcgctac acaagccctg cccatagctt ttaggacatg ttgaatagtt
      121 gctaaaaatt gtacctttgc cgaaaagaaa gctatgcaca aatcgctgtt actcttcatt
      181 gtactgatgc tgacgaccat cggagctatg gcgcaaggca aagtcaccgt caaagaagac
      241 cccgtcatca cgcagatgat ggaccgctac acgcaaacca acaaggcccg cagaaccgtg
      301 gacggctggc gcgtgcaaat catggccacg cccgacaggc aaaatctgga ccgcagcctg
      361 caatcgttcc agtaccgcta tcccaacttg tctgccgact gggttcacaa caacccgtac
      421 tacaaaatcc gcgtgggcgc cttctctacc cgactggagg ctcagcgcct gctgaatatt
      481 ctcaagccgg actatccggg cgcttacctc gtggccgaca accagatgaa acccgccgaa
      541 ctcttaggaa cctactgatt cactatgcct acatcttccc ggcacggctg gcgcaacatt
      601 gactgggttg cgttttccat ttttctgagc ctcgtggcca tcggttggct catggtttat
      661 accgtgaact accccgaatt ggagcgcggc gtcaattttt tcagttctac tgccggcaaa
      721 caaacggtgt ggcttttcat cgctatgatg gtgttcgctg tggtgatggt gatagactgg
      781 aagttttggc aaaccttcaa cctgcccatc tacgtcggcg gcatactctt gttgatcggc
      841 gtactgtttt tgggttccaa catcaaaggc gccacctcgt ggtttgcctt cggaggcttt
      901 tcatttcaac cctccgagtt ggccaaagta ggtacggcgc tggcattggc cggttatctg
      961 agcaggtatg gcaccaatct ccgctttgcg cgcagccagg ccattgtatt gggaatcatc
     1021 atcattccca tgattttgat tttatggcaa cccgatgccg gctcggcgct cacttttctg
     1081 tcgttctttt tcctgctgta tcgcgagggg ctttcgccca atatttacgc ggtgggcttc
     1141 ttcctggcag gtgtggccct gctgagtttg ctgttcaatc ccatcctgat acccatactc
     1201 atcacgctgg gcggatttta tgtgctggcc ggcaatttcc gggagccgaa gcactggcgt
     1261 cgggcggcgc tggcggcgtc ggtattggcg gctgtggcgg ttttgggttt caaacagtac
     1321 tggattgcac tgggactggg tctgacgggt tttctcgtgg ccggctttct gcacttagcc
     1381 cgttacaatg cacgattggc cggtatgctc acggcggtac tggtggtggg cagcggcctg
     1441 gcgttttcta cggattatgc cttcaacaac atcatgaaac cgcaccagca agaccgcatc
     1501 aatgtgtggc tgcaccccga acgctgcgac cccagaggct cgctgtacaa tgtattgcag
     1561 tctaaaatgg ccatcgcttc gggcggtctg gagggcaagg gctttctgga gggcgcgatg
     1621 accaagatga attatgtgcc cgaacaaagc accgacttta tcttctgcac ggtaggggag
     1681 gagcagggtt tcatcggcag catgacggtc attgttttgt ttctggcctt cttgttgcgc
     1741 ctcattttta ttgccgaaag gcagcgttct ccgttcagcc ggcactttgc gtatggcgtg
     1801 gcggggctgc tgtttgttca cttttttgtc aatatcggca tgacgatggg attgctgccc
     1861 atcatcggca ttcccttgcc gctcatcagc aaaggaggct cgtctttgct gggcttcacc
     1921 attatgatcg gcattttgct caaattggat tccaatcgtt acagcctcta aaaactcacg
     1981 ctttgttttc atttacgcta caacataccg acccgcactc caaagcgcgc gcgggcctca
     2041 tccaaacggc gcatggcgaa atagccacgc ccatttttat gcctgtgggc acggtgggca
     2101 cggtgaaggc cgttcacctg cacgaactcc gcgatgagat cgaagcgcaa atcattttgg
     2161 gcaataccta ccacctctac ctgcggcccg gcttggacat catacgcggc gcgggcggcc
     2221 tgcatcgctt catcggttgg gaacgcccca tgctcaccga cagcggcgga tatcaggtgt
     2281 atagcctcag cggcacgcgc aaaattgccg agcagggcgt taccttcagt tcgcatattg
     2341 acggctcgaa gcacgtcttc accccggagg gcgtgatgga cattcagcgc gtcatcgggg
     2401 ccgacatcat catggctttc gacgagtgcc cgccgtaccc cagcgattac aaatacgccc
     2461 gcaattcgat ggagatgacg caccggtggt tgcagcgctg catccagcgg tttgatgaaa
     2521 cggagggtct gtacgggtac gaacaaacgc tcttccccat cgtgcagggc agtacctacg
     2581 aagacctgcg cactgcctcg gcagagacca tagcggcagc cgggcggccc ggcaatgcca
     2641 tcggcggcct gtcggtaggc gagccggagg aggagatgta ccgtttcacg gaattggtct
     2701 gcaacatcct gcccaaagac aaaccgcgct acctcatggg cgtgggcact ccggtgaaca
     2761 ttctggaagg cattgcgctg ggcgtggaca tgttcgactg cgtgatgccc acccgcaatg
     2821 cgcgcaacgg ctggctcttc acccgctatg gcgacgtgaa gatcaagaac gcccgccaca
     2881 agaccgacca ccagccgctg gacgccacct gcacctgcta cgcctgcgcc ggcaaggccg
     2941 gcgtgaactg ggccgacggc gggcgtgacg gcttcagccg cgcctacctg caccacctgg
     3001 accgctgcgg cgagatgctc ggcccga
//
LOCUS       DAORLF010000524         3025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17763_length_3025_cov_1.885859, whole genome shotgun sequence.
ACCESSION   DAORLF010000524 DAORLF010000000
VERSION     DAORLF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17763_length_3025_cov_1.885859"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>409)
                     /locus_tag="PK198_25205"
     CDS             complement(<1..>409)
                     /locus_tag="PK198_25205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF42125.1"
                     /translation="EITIDRQKAQALGVSMLDVAQTLQLAFSGSRFGYFILNGKQYQV
                     IGQVARSDRNKPADLRRLYVRNNRGQMVQLDNLINTTESSAPPALYRYNRFISATVSA
                     GLAPGKTLGEGIDAMQAIADRLLDDTFDTDLSGP"
     assembly_gap    410..424
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<425..>1846)
                     /locus_tag="PK198_25210"
     CDS             complement(<425..>1846)
                     /locus_tag="PK198_25210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF42126.1"
                     /translation="QGRLSSPEEFNQLIIKQQDGSLIRFRDLGYAELAPENLRTLLRR
                     DGKSMVGCAVTPQPGTNHIEIADEVYKRLTQIQKDLPKDITASMGFDTTKFIRNSIKE
                     VQETIYLAFGLVVLIIFLFLRDWRTTLIPVLAIPISLIGAFFVMYLAGFSINVLTLLG
                     IVLAIGLVVDDAIVVLENIYAKIEAGKAPLQAALTGSREIFFAVISTTVTLVAVFLPV
                     IFLQGLTGRLFREFGAVIAGAVIISSFVALTLTPMLSGRILKKRAVEPLFYRLTEPFF
                     QWLNNLYRRSLEGFFRVRWMALPIMAAAVGMIFYFGKQVPSELAPNEDRSRLRAMVST
                     AEGSTFEYTDSVVMDLYKIVSKEVPERDGITSVTAPGFGATSAANAGFVNLILKEPTE
                     RKRSQQQIMDELMPKISGLSRARIFITQEPTISVGGNRRGGLPVQMVIQAPNFDKLKS
                     IMPAFLAEARQNPTFTIVDVDLK"
     assembly_gap    1847..1984
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1985..2535)
                     /locus_tag="PK198_25215"
     CDS             complement(<1985..2535)
                     /locus_tag="PK198_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF42127.1"
                     /translation="MSLASISIQRPVLAIVMSVVIVLFGVIGMQSLGIREFPSVEPPI
                     ITVSCGYVGANADIIESQITEPIEEAVNGIAGIRSITSTSSNGRSNITIEFTLGEDLE
                     TAANDVRDKVSQVVRQLPVDADPPVVSKADADASPIVFITVFSEERSLLELSEFAENV
                     FKERMQTIPGVSTVNIWGQKKYS"
     gene            complement(2612..>3025)
                     /locus_tag="PK198_25220"
     CDS             complement(2612..>3025)
                     /locus_tag="PK198_25220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF42128.1"
                     /translation="GTVVRFRMDGSDKTYTASVLAREPQIDPATRTLRVKASAPNSDG
                     RILPGAFAAVSVGLKDYDRTIMIPTEAVIPEEGAQKVFVYRNGMAESVKIETGIRKNT
                     EIQVLHGLQEGDTLITSGVMLIRPGAPVKIESLRD"
BASE COUNT          636 a          742 c          833 g          661 t
ORIGIN      
        1 aaggtccgct caggtcggta tcaaacgtat cgtcgagcag tcggtctgca atggcctgca
       61 tggcgtctat gccctcgccg agggttttgc cgggcgccag gcctgccgaa acagtggccg
      121 aaatgaagcg attgtagcga tacaaggccg gcggagcact gctttcggtg gtgttgatga
      181 gattgtccaa ctgcaccatt tggccccgat tgttgcgcac ataaagacga cgcagatcgg
      241 cgggtttgtt gcggtcgctg cgggccactt gcccgataac ctgatactgt tttccgttga
      301 gaatgaaata gccaaagcgc gaaccgctga atgccaactg gagcgtttgc gccacgtcga
      361 gcatggaaac gccgagggct tgggcttttt ggcggtcaat ggtgatttcn nnnnnnnnnn
      421 nnnnatttca aatcaacgtc cacaatggtg aaagtaggat tctggcgggc ttccgctaaa
      481 aaggccggca tgatggattt cagcttgtcg aagttgggcg cctgaataac catttgcacc
      541 ggaaggccgc cccggcggtt gccgcctacg ctgatggtgg gttcttgcgt gatgaagatg
      601 cgcgcgcgac tgagaccgct gattttcggc atcagctcgt ccatgatctg ctgttggctg
      661 cgtttgcgtt ccgtgggttc tttgaggatg aggttgacga agccggcatt ggccgccgag
      721 gtagcgccga agccgggcgc tgttacagat gtaattccgt ctctttcagg tacttccttg
      781 cttacaattt tgtataagtc catgaccaca gagtcggtgt attcaaacgt cgaaccttcc
      841 gcagtagaga ccatagcgcg caaacggctg cggtcttcat tgggcgccag ctcggacggc
      901 acctgtttcc caaaataaaa aatcatgccc acggctgccg ccatgatggg aagcgccatc
      961 caacgaacgc ggaaaaagcc ttcgagcgag cgccggtaaa ggttgttgag ccattgaaaa
     1021 aaaggttcag tcagcctgta aaacagaggc tctacggccc gttttttcaa tatccgcccc
     1081 gacaacatcg gcgtcagcgt aagcgctaca aaagaggata tgatgaccgc gccggcgata
     1141 acggctccaa actcgcggaa caagcgcccg gtgaggccct gaaggaaaat cacgggcagg
     1201 aacacggcca ccagcgttac ggtggtggaa atcacagcaa agaaaatctc gcgcgaaccg
     1261 gtgagggccg cctgcaaggg ggctttgccg gcttcaattt tggcataaat attttcgagt
     1321 accacaatgg cgtcgtccac taccaagccg atggccaaaa cgatgcccaa aagcgtgagc
     1381 acattgatgg agaaaccggc gaggtacatc acaaaaaatg ccccgatgag cgagatggga
     1441 atggccaata ccggtatgag cgtagtgcgc cagtcgcgca aaaacaggaa gatgatgagt
     1501 accaccagac cgaaagccaa atagatggtt tcctgcacct ctttgatgga gttgcggata
     1561 aacttggtgg tgtcaaatcc catggacgcg gtgatgtctt tggggaggtc tttctgaatc
     1621 tgagtcaggc gtttatacac ctcgtcggct atttcgatgt ggttggtgcc cggttgtggc
     1681 gtgacggcgc agcctaccat agactttcca tcgcggcgga ggagggtgcg caggttttcg
     1741 ggcgccaatt ctgcatagcc cagatcgcgg aagcggatga ggcttccgtc ttgttgtttg
     1801 ataatgagct gattaaattc ttccgggctg cttagtcggc cttgtgnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnatggag tatttttttt gcccccagat gttgacggtg ctcacgccgg ggatggtttg
     2041 catgcgttct ttaaacacgt tttctgcaaa ctcgctcaat tccagcaaag accgctcttc
     2101 gctgaaaacc gtaataaata caataggcga cgcatcggca tcggctttgg aaaccaccgg
     2161 cgggtcggca tccaccggga gttgccgcac cacctgcgat accttgtcgc gcacgtcgtt
     2221 ggccgccgtt tcgaggtctt ctcccagcgt aaattcaatg gtgatgttgc tgcggccatt
     2281 gctgctggtg gacgtgatgc tgcgaatgcc ggcaataccg ttgaccgcct cctctatcgg
     2341 ctccgtgatc tgcgattcaa tgatgtcggc attggcgccc acatacccgc aggataccgt
     2401 gataatgggc ggctctacgc tgggaaactc ccgaatgccg agggattgca taccgatgac
     2461 gccgaacaat acaatgacca ccgacataac gatggccaat accgggcgct gaatactgat
     2521 ggatgctaaa ctcatggtgt tggattatca ggttgatcgg ttaatctcaa aagcgtgaca
     2581 atgaatgatt tattgggaga tgcgattccg cttaatccct caaactctca atcttcacag
     2641 gtgcgccggg ccgaatgagc atcacgccgg aggtgatgag cgtgtcgcct tcctgcaaac
     2701 cgtgcaatac ctgaatttcg gtgtttttcc gaatgccggt ttctattttg acagactccg
     2761 ccatgccgtt gcgatacaca aatactttct gtgcgccctc ttccgggatg acggcttcgg
     2821 taggaatcat gatggtgcgg tcgtaatctt tcagtcctac cgatacggcg gcaaatgcgc
     2881 ccggcaggat gcgcccgtcg gagttgggcg ccgatgcttt gacccgcagc gtgcgcgtgg
     2941 cggggtctat ttgtggctcg cgggccaaca cggaggcggt gtaggttttg tcggagccgt
     3001 ccatgcggaa tcgtaccacc gtgcc
//
LOCUS       DAORLF010000525         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17843_length_3015_cov_1.839189, whole genome shotgun sequence.
ACCESSION   DAORLF010000525 DAORLF010000000
VERSION     DAORLF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17843_length_3015_cov_1.839189"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1423)
                     /gene="tilS"
                     /locus_tag="PK198_25225"
     CDS             complement(74..1423)
                     /gene="tilS"
                     /locus_tag="PK198_25225"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRF42129.1"
                     /translation="MLEKFTHHLRTACRLNGTERVLAAVSGGLDSMVMAHLLRLAGWP
                     HGIAHCNFRLRGAESDADEALVRAYAEAHEIPFFSTAFDTAEIAAEAKESIQITARRL
                     RYEWFEQIRAANGYDFIATAHHQDDAAETLLYNLSKGCGIRGLHGIAQRSGYLLRPLL
                     FAGRSEWKAFARLENVVWREDASNQESYYARNKIRREAVPVFHSINPQWTQTMAANIQ
                     RFRDAEALMQWAVEQMAAQITSTLPSGGLRIRLDRLSEFPAPATVLYEILRPYGFGAS
                     QARAILEARGRQSGALFMAARHRLTLHQGDAIVEALPAHEVTTWEWSEAQPVISLPEG
                     TLFLEKKEGRPEQLTSSPYTALLDADRTAMPLRIRPWQPGDRFCPLGMEGRSKKLQDL
                     FTDLKLSRFEKERVRVIETAGGEICWVAGYRADDRFKITADTKSYFVLRFEKNEPNL"
     assembly_gap    1482..1491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1492..>2635)
                     /locus_tag="PK198_25230"
     CDS             complement(<1492..>2635)
                     /locus_tag="PK198_25230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF42130.1"
                     /translation="LIHNSEPGLLIVESKFQHLLDAAPNSAAVPHHWSFEALADFCHP
                     ATHTDEDSPFPIVPVDEDAPLFILYTSGTTGFPKGALYTHKMLFWNSINTAMSLIVNS
                     ESRTVNVMPPFHTGGWNVLTTPFLHHGGYTISMRKFDPSAVLQVLQDEQLSLFMAVPT
                     MLKMMADAPEFEAAQFPHLRYFIVGGEPMPIPLIERWDSKNVPVRQGYGMTEVGPNLT
                     SLHQDDAIRKKGSIGRPNFYVQIRIVDETGQDLPPDAPGELLLKGPMVTPGYWRNEEA
                     TRKTITEGWFHTGDMVRMDADHYIYVVDRIKNMFISGGENVYPAEIERILLTHPAVSE
                     SAVIGVADEKWGEVGHAFVVLKAGEMCTEAALLEFCKEKLARFKVP"
     assembly_gap    2636..2712
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2713..2929)
                     /locus_tag="PK198_25235"
     CDS             complement(<2713..2929)
                     /locus_tag="PK198_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF42131.1"
                     /translation="MQTLAQDWAAKWAMYSPDKPAVKEYETGRTLTYGQLNRLANRLA
                     RHLSSAYGIHKGDRIALLSENCNEYIVL"
BASE COUNT          574 a          751 c          915 g          688 t
ORIGIN      
        1 cttgcaaaac gatgctgaac aggctgaaaa tgaggagtgt aaagacgttg cgattcatgg
       61 tcattggatt tttttacaaa tttggctcgt ttttttcaaa cctgagtaca aagtagcttt
      121 ttgtgtcggc agttattttg aatctgtcgt cggcgcgata gccggccacc cagcaaattt
      181 cgcccccggc ggtttctatc acccgtaccc gttccttctc aaaacgcgat agttttaggt
      241 cggtaaacaa atcctgcaac ttcttgctgc ggccctccat gccgagcggg cagaagcggt
      301 cgcccggctg ccagggccga atgcgcaggg gcatggcggt tcggtcggcg tcgagcaggg
      361 ccgtgtaggg cgaggaggtc agttgttcgg ggcggccttc ttttttttct aaaaacagcg
      421 tgccttccgg caaagaaatc accggctgcg cttccgacca ttcccatgtt gtgacttcat
      481 gggcgggcaa ggcttccaca atggcatcgc cctgatgcaa agtcagtcgg tgccgggctg
      541 ccataaacaa tgcgcccgac tgcctgccgc gcgcttcgag gatggcccgg gcctggcttg
      601 cgccaaatcc gtaaggccgc agtatttcgt acagcacggt ggccggcgcc ggaaattccg
      661 acaaacggtc gagccggatg cgcagccccc ccgacggcag ggtacttgtg atctgcgccg
      721 ccatttgctc caccgcccac tgcatcagcg cctcggcatc ccgaaaccgt tgtatgttgg
      781 cggccatggt ttgcgtccat tgcgggttga tgctgtggaa aaccggcacg gcctcgcggc
      841 gaattttgtt gcgggcatag tagctctcct gattggaggc gtcttctctc caaacgacgt
      901 tttcgagccg ggcaaaggct ttccattcgc tccgtccggc gaagagaagc ggccgaagga
      961 ggtacccgct gcgttgggca atgccgtgca aaccgcggat gccgcagcct ttgctcaggt
     1021 tgtacagcag ggtttcggcg gcgtcgtcct ggtggtgggc ggtggcgatg aagtcgtagc
     1081 cgttggcggc gcgtatctgt tcaaaccatt catagcgcag gcgacgggca gttatttgta
     1141 tagattcttt ggcctcggcg gcaatttcgg cagtatcgaa agcagtactg aaaaaaggaa
     1201 tttcgtgcgc ctctgcgtaa gcgcgcacca aggcttcatc ggcatccgac tcagcgccgc
     1261 gcagcctgaa attgcagtgc gcaatgccat gcggccatcc ggcaagtcgg agcaaatggg
     1321 ccatgaccat actatctaaa ccaccgctca cggctgccaa tacgcgctct gtgccgttga
     1381 gccggcaggc ggtacgcagg tgatgggtga atttttcgag catgatttat tttcctttca
     1441 gcgccatccg gttgattttg ccggtgtcgt ttttgggcag gnnnnnnnnn nttcggcact
     1501 ttgaaacggg ccaatttttc tttacaaaac tccaacaggg ccgcctccgt acacatttca
     1561 ccggctttga gtaccacaaa agcatggccc acctcgcccc atttttcgtc ggctacaccg
     1621 atgaccgccg attcggaaac tgccgggtgc gtgagcaaaa tgcgctctat ttcagcggga
     1681 tatacgtttt caccgccgga gatgaacatg tttttgatgc ggtccaccac atagatgtag
     1741 tggtcggcat ccatgcgcac catatcgccg gtatggaacc agccctcggt gatggtcttg
     1801 cgggtggctt cctcgttgcg ccagtagccg ggtgtcacca tcgggccttt gaggaggagt
     1861 tcgccggggg catcgggcgg gaggtcctgg ccggtttcat ccacgatgcg gatttggaca
     1921 taaaaattgg gtctgccgat ggagcctttt ttgcgaatgg cgtcgtcctg atgcagggag
     1981 gtgagattgg ggccaacctc ggtcatgccg tagccctggc gcacggggac gtttttgctg
     2041 tcccagcgct caatgagcgg aatgggcatg ggttcgccgc cgacgatgaa gtagcgcaag
     2101 tgagggaatt gagcggcttc aaattccggg gcatcggcca tcattttgag catggtgggc
     2161 acggccatga acagcgaaag ctgctcgtcc tgaagcactt gcaataccgc cgagggatcg
     2221 aatttgcgca tcgaaatggt gtagccgccg tgatgcaaaa agggcgtggt gagcacattc
     2281 cagccgccgg tatggaaggg cggcatgacg ttgacggtgc ggctttctga gttgacaatc
     2341 aggctcatgg ccgtgttgat gctgttccaa aagagcattt tgtgggtata aagcgcgcct
     2401 ttgggaaagc ccgttgtgcc cgaagtgtag aggatgaaca ggggggcgtc ttcgtccaca
     2461 ggcacgatgg ggaaggggct gtcttcgtcg gtgtgagttg ccggatggca gaagtcggcc
     2521 agcgcctcga atgaccagtg atgcggaacg gcggcgctgt tgggcgcggc atcgagcagg
     2581 tgctggaatt tggattcgac gatgagcagg ccgggttcgg aattgtggat gaggtnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnaaaggacg atgtactcgt tgcagttttc ggagagcaga gcgatgcggt
     2761 cgcctttgtg aatgccgtag gcggaagaca ggtgccgggc taagcggttg gccaagcgat
     2821 tgagttgtcc ataagtcagg gtacgccctg tttcgtattc tttgaccgcc ggtttgtcgg
     2881 ggctgtacat ggcccatttg gcggcccagt cttgcgctaa agtttgcatg ttttgcttgc
     2941 tgctgatggt tgttttgaat gaagaccgaa gttagaacgt ccgattcaat tggccaaata
     3001 ttttttggag aaggg
//
LOCUS       DAORLF010000526         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_17931_length_3002_cov_2.008144, whole genome shotgun sequence.
ACCESSION   DAORLF010000526 DAORLF010000000
VERSION     DAORLF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17931_length_3002_cov_2.008144"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>395)
                     /locus_tag="PK198_25240"
     CDS             complement(<1..>395)
                     /locus_tag="PK198_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF42132.1"
                     /translation="EEAEGVQVAILLHDIGHGPFSHTLEHSLINVSHETLSLLFMEAL
                     DEAFDGRLTLARRIFDGSHPKHFLHQLVSGQVDMDRMDYLNRDSFFTGVHEGVIGYDR
                     IIKMLEVRDNELVVEEKGIYSVEKFLIAR"
     assembly_gap    396..460
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<461..652)
                     /locus_tag="PK198_25245"
     CDS             complement(<461..652)
                     /locus_tag="PK198_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRF42133.1"
                     /translation="MVKGKILNDPVYGFINVPYGLLFQLAEHPWFQRLRRIKQMSLAH
                     YVYPGALHTRFHHALGALHL"
     gene            686..916
                     /locus_tag="PK198_25250"
     CDS             686..916
                     /locus_tag="PK198_25250"
                     /inference="COORDINATES: protein motif:HMM:NF033487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6435 family protein"
                     /protein_id="HRF42134.1"
                     /translation="MGTKQRALCCISQNQINMFGLFKKDPVKEWQKQYRELMEEAMHI
                     QRSGDLRAYAAKIEEAEAVAKKIEEAAAGKKE"
     gene            1163..1606
                     /gene="rplM"
                     /locus_tag="PK198_25255"
     CDS             1163..1606
                     /gene="rplM"
                     /locus_tag="PK198_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRF42135.1"
                     /translation="MNTLSYRTETPNPAEVDRKWFVIDADGEIVGRLSTKIAHVLRGK
                     HKPSYATNVDVGDFVIVINADKMRFTGNKVNDKVYTTYSGYPGGQRKTTAAEMLVKKP
                     HEVIEHAVKGMLPKTKLGRAMFKKLFVYAGAEHPHQAQKPEPFNF"
     gene            1628..2017
                     /gene="rpsI"
                     /locus_tag="PK198_25260"
     CDS             1628..2017
                     /gene="rpsI"
                     /locus_tag="PK198_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRF42136.1"
                     /translation="MEMINAIGRRKSSVARVYLSKGDGKILINGKDYKAYFPQTHVQA
                     NITQPFATAAIENGIYDLNVNVKGGGFKGQSEAVRMGISRALIKLNEDFRKPLKEQKL
                     LTRDAREVERKKYGKPKARKSFQFSKR"
     gene            2060..>2301
                     /gene="rpsB"
                     /locus_tag="PK198_25265"
     CDS             2060..>2301
                     /gene="rpsB"
                     /locus_tag="PK198_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRF42137.1"
                     /translation="MEKPTYKDLLDAGVHFGHLRKKWNPKMSPYIFTERKGIHIIDLN
                     RTLDSMERAAAAMRQIAKSGRKVLFVATKKQARDIVA"
     assembly_gap    2302..2328
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2329..2786
                     /gene="rpsB"
                     /locus_tag="PK198_25270"
     CDS             <2329..2786
                     /gene="rpsB"
                     /locus_tag="PK198_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRF42138.1"
                     /translation="VTERWWGGMMTNFATIRRSIKKMNNIDRMLSDGTLASVTKKEKL
                     TLTRERNKLEKVLGGIANLTRLPAAVFVVDIEHENIAVAEANRLGIRTFGIVDTNSNP
                     NDVDFAIPGNDDASKSIAIITNYMVNAIRQGLDERKQAKPEKDGVAEEA"
BASE COUNT          831 a          744 c          726 g          609 t
ORIGIN      
        1 ggcgcgcgat gaggaatttt tcaacggaat aaatgccctt ttcctccacc accagttcat
       61 tgtcgcgcac ttccaacatt ttgatgatgc ggtcgtagcc gatgacgccc tcgtgtacgc
      121 cggtgaaaaa gctgtcgcgg ttgagataat ccatgcggtc catgtctacc tgaccggaga
      181 cgagctgatg caaaaagtgt ttgggatgcg aaccgtcgaa aatgcggcgg gctaaggtca
      241 gccgtccgtc gaatgcctcg tccaaagcct ccatgaacaa cagggagagc gtttcgtgcg
      301 atacgttgat gagcgagtgt tccagcgtgt gcgagaacgg cccgtgcccg atgtcgtgca
      361 gcaaaatggc cacctgcacg ccctctgctt cttcgnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggtgcagc gcgcccagcg
      481 catgatgaaa gcgcgtgtgc agcgcgccgg gatatacata atgcgccagg ctcatttgct
      541 tgatgcggcg caggcgttga aaccacgggt gttcggccaa ctgaaaaagc aagccgtacg
      601 gcacgttgat gaatccatat acaggatcgt tgagaatttt tcctttcacc atctgccttt
      661 gatttgggtg caaagttgcg gaaaaatggg aacaaaacag cgggcgctct gttgcatctc
      721 ccaaaatcag atcaatatgt tcggactgtt caaaaaagac cctgtaaagg aatggcaaaa
      781 gcaatatcgc gaactgatgg aagaggcgat gcacatacag cgcagcggtg atttgcgggc
      841 ttatgcggcc aaaattgaag aagctgaggc ggtggcaaag aaaatagaag aggctgctgc
      901 cgggaaaaag gaatgatgag ggagggggat taaacccctg caacacgcaa cccgctgaaa
      961 aacagccgga aattcgccct gcctcgtcca cctctcctgc ctctttgaaa aaaaatgttc
     1021 caaaaaaata ctgtttatca aggagttata aaaaatagag aaagaaattt ttccccatgt
     1081 cttgtttaat caaaaagctg catttacctt tgcactcgct tttgaaaagc ggttttaaaa
     1141 aagaattcaa aaacaactga acgtgaatac attaagttat cgcacggaga cgcccaaccc
     1201 ggcagaagtg gaccgcaaat ggttcgtaat tgacgccgac ggagagattg taggccgtct
     1261 cagcaccaag atcgcccacg ttttgcgggg caaacacaaa ccgagctacg ccaccaacgt
     1321 ggatgtcggc gacttcgtca tcgtgatcaa cgcagacaaa atgcgtttca ccggcaataa
     1381 ggtcaacgac aaggtttaca ccacctattc gggctatccc ggcggacagc gcaagaccac
     1441 tgctgctgaa atgctcgtca aaaagccgca cgaagtcatt gagcacgctg taaaaggcat
     1501 gttgcccaag acgaaactgg gccgcgcgat gttcaaaaaa ctttttgtct atgccggcgc
     1561 cgagcacccg catcaggctc aaaaaccgga accattcaac ttctaaaaaa tacgcacagc
     1621 gatttatatg gaaatgatca atgcaatagg gaggagaaaa tcttctgtag cgcgggtata
     1681 tctcagcaaa ggcgacggca agatcctcat caacgggaaa gattataaag cctacttccc
     1741 gcaaacacac gttcaggcca acatcacgca gccgttcgct actgccgcca tcgaaaacgg
     1801 catttacgac ctcaacgtca acgtcaaagg cggtggcttc aaaggtcagt cggaggcagt
     1861 tcgtatggga atttcacgcg cgcttatcaa gctcaacgag gacttccgca agccgctgaa
     1921 ggagcaaaaa ttgctcactc gcgacgctcg cgaagtagaa cgcaagaagt acggcaagcc
     1981 caaggcacgc aagagcttcc agttcagcaa gcgttaatgc ttttttcttc ctcattcaaa
     2041 attttctcaa tacaggacaa tggaaaaacc tacctataaa gacttgttgg atgcgggcgt
     2101 tcacttcggc cacctgcgca aaaagtggaa tcctaaaatg tctccgtata tctttactga
     2161 gcgcaaaggc attcacatca ttgatctcaa tcgcaccctc gactccatgg agcgcgccgc
     2221 cgctgctatg cgtcaaattg ctaagtcggg ccgcaaagtg ctcttcgtag ctaccaaaaa
     2281 acaagctcgc gacatcgtag cnnnnnnnnn nnnnnnnnnn nnnnnnnnat gtgaccgagc
     2341 gttggtgggg tggcatgatg accaacttcg ccacgatccg ccgctccatc aaaaagatga
     2401 acaacattga ccgcatgttg tccgacggca ccttggccag cgttaccaaa aaggaaaaac
     2461 tgacgctgac ccgcgagcgc aacaaactcg aaaaagtact cggcggtatt gccaatctca
     2521 cgcgcctgcc tgctgccgtt tttgtggtgg acatcgagca tgagaacatt gcagtagctg
     2581 aagcgaaccg cctcggcatc cgcactttcg gcatcgtgga caccaactcc aatccgaacg
     2641 atgtggactt cgccattccg ggcaacgacg acgcttccaa atccatcgct atcatcacca
     2701 actatatggt gaatgccatc cgccagggtt tggacgagcg caagcaagcc aagccggaga
     2761 aagacggcgt agctgaagaa gcctgatttc gaggcgtttg tgtcaagata taaggcagcg
     2821 gactgagatt gcaggaaaat tttaccgatc tgccatttta tcacttttta atcaaaatat
     2881 caaatgagtg caatatctgc aggcgatgta aaacaactgc gcgaaatgac cggcgcaggt
     2941 atgatggact gcaaagccgc cctctctgaa gcggaaggcg attttgataa agccattgaa
     3001 at
//
LOCUS       DAORLF010000527         2962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18239_length_2962_cov_1.986928, whole genome shotgun sequence.
ACCESSION   DAORLF010000527 DAORLF010000000
VERSION     DAORLF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_2962_cov_1.986928"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>823
                     /locus_tag="PK198_25275"
     CDS             20..>823
                     /locus_tag="PK198_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF42139.1"
                     /translation="MKTIKGPAIFLAQFMGDEAPFNSLEGICKWAAELGYKGIQIPTW
                     ESRLIDLKTAAESKTYCDELKGKIAGYGLEISELSTHLQGQLVAVHPAYDSLFDAFAP
                     AEVHGQPKARQEWAVQQLKYAARASQHLGLTAHATFSGALLWPYMYPWPQRPQGLAET
                     GFAELAARWMPILDSFDAAGVDLCYEIHPGEDLHDGVTFERFLAAASEHPRINILFDP
                     SHFVLQQMDYLQFIDIYQEFIRMFHVKDAEYQRNGRTGVYGGYLDWAERA"
     assembly_gap    824..862
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <863..1078
                     /locus_tag="PK198_25280"
     CDS             <863..1078
                     /locus_tag="PK198_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRF42140.1"
                     /translation="DFKGIFSRLSQYDYDGWAVMEWECCIKSPEQGAREGAEFISRHI
                     IEVTDKTFDDFAGGKSNEAANRRLLGI"
     gene            1154..1969
                     /locus_tag="PK198_25285"
     CDS             1154..1969
                     /locus_tag="PK198_25285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42141.1"
                     /translation="MSFKTYLFLSFAAIFLLGGCLVIDDAYNGLPPGKWRGVVQLEPS
                     YITPNPKGKRLPEKLNLEFEDVTVGELPFQFTVSYPTPDSFVIALHKAGGEIILSDYT
                     IGRDFSTAKDTLTIRFPGTTNYIRGVFEEKILEGHWFYTHPSGEQKSIPVIARQGQDY
                     LFTTLRKTPEADVNGDWNLLVNVDSEQPEPAILSLKAKENELQGTLEWQGRRYEALSG
                     TIQARKLYLSFFDGRDALLLEAKVMENGELYGALASGGSGKMLWQANKNQRNQ"
     gene            1973..>2962
                     /locus_tag="PK198_25290"
     CDS             1973..>2962
                     /locus_tag="PK198_25290"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRF42142.1"
                     /translation="MIHQQLTLAYSSENARAAAILEQQLQTAGYAFQHVVDNKDEGNR
                     SLFERLEGAEGKVVVLISDNFLKSTSCMNGALRFFQNNVSRVLPVVVEGSAVDDATGA
                     VISVPAHFERVSDIIQYINYWQDRYLDVRRQKRHLEGIDEEGFNSHLKIMRDISSEIG
                     EFLRWLRSAEFVHLTDLQSSNYERFFRFTGDEDKIWTFKTPPTADSTPHSDELPDIDL
                     SDIPGISLLPDEPIATEEEEPALAKDESQEADVAPDTEDATPGETEMPAAPEITGEAP
                     ASDEPEPEALNPESVGGEQLAVGGDNLEPETRNLKPETQNSEPVGGEQLAVERS"
BASE COUNT          739 a          811 c          758 g          615 t
ORIGIN      
        1 tgaaaacaaa acgacaaaga tgaaaacgat caaaggaccg gccatttttc tggcgcagtt
       61 tatgggcgat gaagccccgt tcaattctct ggaaggtatt tgcaaatggg ctgccgaact
      121 ggggtacaaa ggcatacaaa ttccgacttg ggaatcgcgg ctgatagacc tgaaaacggc
      181 ggcagaaagc aaaacttact gcgatgaact gaagggaaaa attgccggtt acgggctgga
      241 aatttcagag ctttccacgc atttgcaggg gcagttggtg gccgtgcatc cggcttacga
      301 cagcctgttc gatgcttttg cgcctgcgga agtgcatgga caacccaaag cccggcagga
      361 gtgggccgtg cagcaactga aatacgcggc acgagcatcg caacatctgg gcctcactgc
      421 tcatgccacc ttttcgggtg cgctgctgtg gccatacatg tatccctggc cgcagcgtcc
      481 acagggtctg gcggagacgg gttttgcaga attggcggcc cgctggatgc ccattttgga
      541 cagtttcgat gcggcgggcg tggatttgtg ttatgaaatt caccccggag aagacctcca
      601 cgacggggtc accttcgagc gttttttggc ggctgcgagc gaacatccgc gcatcaatat
      661 cctgttcgac cccagccatt ttgttttaca acagatggat tatctgcaat ttatagacat
      721 ttaccaagag ttcatccgca tgttccatgt caaggatgcc gagtaccagc gcaatggccg
      781 cacgggcgta tatggcggtt atttagactg ggccgagcgt gccnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nngattttaa aggcattttc agtcgcttgt cgcaatacga
      901 ttacgacggc tgggcggtca tggaatggga atgttgcatc aaaagcccgg agcagggcgc
      961 gcgcgagggg gcggagttca tcagtcggca catcatagaa gtgacggata aaaccttcga
     1021 cgattttgcc ggcggaaaaa gcaatgaagc cgccaaccgc cgcttgttgg gcatttgaga
     1081 ttcactacaa ttttccgctg tgaaccaagc ccttgccggt tcggtttaca aaccgcacaa
     1141 gaacagcaac aaaatgtcat tcaaaacata ccttttccta tcattcgcag ccatttttct
     1201 gctcggcggt tgtctcgtta ttgacgacgc ctacaacggc cttcctcccg gcaaatggcg
     1261 cggcgtagtg caattggaac cttcgtacat cacgcccaac cccaaaggca aacggctgcc
     1321 cgaaaagctc aatctggagt ttgaagacgt cacggtggga gagttgccat ttcaattcac
     1381 cgtctcctac cctacccccg acagcttcgt catcgcgctg cacaaggccg gcggcgaaat
     1441 cattttgagc gattacacca tcggcagaga tttttctacc gccaaagaca cgctcaccat
     1501 tcgctttccg ggcacgacca actacatccg gggtgttttt gaagaaaaaa tcctggaagg
     1561 gcactggttt tacacccatc cctcgggcga gcaaaaatcc atccctgtca ttgcccggca
     1621 agggcaggat tacctcttta ccaccctgcg aaaaacaccg gaagccgatg taaacggaga
     1681 ctggaacctg cttgtcaatg tggattccga acaacccgaa cccgctattt tatcgctgaa
     1741 agccaaagaa aatgagcttc agggcacttt ggaatggcag ggacgacgtt atgaagcctt
     1801 gtcgggcacc attcaggcac gaaaattgta cctttcgttt ttcgatggcc gggatgccct
     1861 gctgctcgaa gccaaagtga tggaaaacgg tgagctgtat ggcgcactcg catcgggcgg
     1921 aagcggcaaa atgctctggc aggccaacaa aaaccaacga aatcaataag tcatgatcca
     1981 ccaacaactc acgcttgctt acagctcgga aaatgcccgg gcagcggcta tcttagaaca
     2041 acaactccag actgcgggat atgcttttca gcatgtcgtg gacaataaag acgaaggcaa
     2101 tcgctctctt ttcgagcggc tcgaaggcgc tgaaggcaaa gtggtcgtac tcatcagcga
     2161 caattttctc aagagcactt cctgcatgaa cggggcgctc cgctttttcc aaaacaacgt
     2221 cagccgcgtt ttgcccgtcg tggtcgaagg ctcagcagtg gatgatgcca ccggggccgt
     2281 catttccgta cctgctcatt ttgaacgggt tagcgacatc attcaataca tcaactactg
     2341 gcaggaccgc tacttggacg tgcgtcgtca aaagcgccac ctcgaaggca tagacgagga
     2401 agggttcaat tcccacctca aaatcatgcg cgatatctcc tccgaaatcg gtgagttttt
     2461 gcgctggctc cgcagtgccg agtttgtcca tctgaccgat ttgcaaagct ccaattacga
     2521 gcgtttcttc cgtttcaccg gcgacgaaga caaaatctgg actttcaaaa cacctcccac
     2581 cgccgacagc acaccgcact ccgacgaact tcccgacatt gacctctccg acattcccgg
     2641 catctccctg ctccccgatg aacccatcgc aacagaggag gaagaacccg ctttggccaa
     2701 agacgaatct caggaagcag atgtcgcacc cgataccgaa gatgctacgc ccggcgaaac
     2761 agaaatgccc gccgcgcccg aaattaccgg cgaagcgccc gcatccgacg aaccggaacc
     2821 cgaagccttg aacccggaat cggtaggcgg tgagcagttg gcggtcggcg gtgacaacct
     2881 tgaacccgaa acccggaacc ttaaacccga aacccaaaac tcggaaccgg taggcggtga
     2941 gcagttggca gtcgagcgaa gc
//
LOCUS       DAORLF010000528         2955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18295_length_2955_cov_2.126552, whole genome shotgun sequence.
ACCESSION   DAORLF010000528 DAORLF010000000
VERSION     DAORLF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18295_length_2955_cov_2.126552"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>221
                     /locus_tag="PK198_25295"
     CDS             <1..>221
                     /locus_tag="PK198_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HRF42143.1"
                     /translation="SNLLQHEAVSGKLNFLPDFNDKQNLIILFIIPLAVQWWSVWYPG
                     AEPGGGGYIVQRMLSAKDEKNAVGATLFF"
     assembly_gap    222..362
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            379..>704
                     /locus_tag="PK198_25300"
     CDS             379..>704
                     /locus_tag="PK198_25300"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42144.1"
                     /translation="MEANPGSVSQEEKDKVQNLRFQANGLTALRQAFPKNTVPDDKIG
                     HDLAFPAMLTKLPGALLGLLLAALIAAYMSTISTHLNWGASYIVNDFYRRFLKPEAGD
                     KELVNVG"
     assembly_gap    705..732
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <733..1308
                     /locus_tag="PK198_25305"
     CDS             <733..1308
                     /locus_tag="PK198_25305"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HRF42145.1"
                     /translation="IISSWLALKLSSALGAFNILLQIGAGTGLLFILRWFWWRISAYS
                     EITAMVVSFLVALFFQFSEFGQSQPDHIKLIIGIAITTTAWVAVTIFTRPTDQAVLLR
                     FYSLIRPHAGGWKPVIEKGRQSGQLSAGDITTGQLPRELASMIAGCFLVYGLLFSMGY
                     LLYGQVMYALPGMAVSAVSVWIIVRSWKKLH"
     gene            1345..2370
                     /gene="pheS"
                     /locus_tag="PK198_25310"
     CDS             1345..2370
                     /gene="pheS"
                     /locus_tag="PK198_25310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRF42146.1"
                     /translation="MLFEQVNSIIAEIEQASPDTPEALEQFRIRFLGSKNVIKALSGE
                     IRNIPNEQKRDFGRLVNEAIAAAEAKFQALKSGLDAAVEQISAASLDLSAPGEPGIWG
                     TRHPISITMNRIIRIFERIGFVVAEDREIEDDWHNFTALNTPEDHPARDMQDTYYLRG
                     SDNLLLRTHTSSVQARLMNVQQPPIRIIAPGRVYRNETISARSHCQFHQVEGLYVDEG
                     VSFADLKQTLLYFAKEMFGATQIRLRPSFFPFTEPSAEMDVYLGTDTEDNYRLTKGTG
                     WLEILGCGMVDPAVLDNCGIDSERYTGFAFGMGIERQAMRLFGINDIRLLFENDTRFL
                     RQFATVV"
     gene            2466..>2955
                     /locus_tag="PK198_25315"
     CDS             2466..>2955
                     /locus_tag="PK198_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRF42147.1"
                     /translation="MKPSIPKGTRDFGPEQVSKRNYMFGIIREVFIRYGYQPIETPTM
                     ELLSTLTGKYGEEGDRLLFKVLNNGDFLSKADEEALQNRDSRKLTASIAEKGLRYDLT
                     VPFARYVVMHQNELSFPFKRYQIQPVWRGDRPQRGRYQEFYQCDVDVVGSNSLMYEAE
                     LVQ"
BASE COUNT          620 a          837 c          707 g          622 t
ORIGIN      
        1 agcaatttgt tgcaacacga ggccgtgagc ggcaagctca actttcttcc ggattttaac
       61 gacaaacaaa accttatcat cctgtttatc atacctttag cggtgcaatg gtggagcgta
      121 tggtaccccg gagcagaacc cggcggcggc ggctatattg tgcagcgcat gctctcggcc
      181 aaagacgaaa aaaatgccgt gggcgccacc ctgtttttca annnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nncgccattg taaaaagaat ggaagccaac ccgggcagcg tgagtcagga ggaaaaagac
      421 aaggtgcaaa accttcgttt tcaggccaat ggcctcactg cgctgcggca ggcattcccc
      481 aaaaataccg ttccagacga taaaatcggc cacgacctcg cttttccggc catgctcact
      541 aaactgcccg gcgctttgtt gggcctgttg ctggcggcgc tcattgctgc gtatatgtcc
      601 accatcagca cgcacctcaa ctggggggct tcgtacatcg tcaacgactt ttaccgccgc
      661 ttcctcaagc ccgaagccgg cgacaaagag ttggtcaacg tgggnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnattatctc ctcctggctg gcgctcaaac tcagcagcgc gctcggcgca
      781 ttcaatattt tgctgcaaat cggcgcgggc accggcctgc ttttcatttt gcgttggttc
      841 tggtggcgca tcagtgcgta cagcgagatc accgccatgg tggtttcgtt tttggtagcc
      901 ctgttttttc agttttcgga gtttggccaa tcgcagcccg accatataaa actcatcatc
      961 ggcattgcca ttaccaccac tgcctgggta gcggtcacga tcttcacccg gcccaccgat
     1021 caggccgtgc tgcttaggtt ttacagcctc attcggcccc atgccggtgg ctggaaaccc
     1081 gtaattgaaa aaggcaggca aagcggccaa ctcagcgccg gcgacatcac caccggccaa
     1141 ctgccccgcg aattggcttc catgattgcc ggctgcttct tagtatatgg cttgctgttc
     1201 tcgatgggct acctgttgta tggtcaggtg atgtacgcgc tgccgggcat ggcggtgtcg
     1261 gcggtatcgg tctggatcat tgtgaggagc tggaagaagc tgcattgagt tttttgtatc
     1321 tttgcagcca cattccatta caacatgctt ttcgaacaag tcaactccat cattgcagaa
     1381 attgagcagg catcgcccga tacgcctgaa gcattagagc aattccgcat tcgctttttg
     1441 ggttccaaaa acgtcatcaa ggccctttcg ggtgagatac gcaacattcc caacgagcaa
     1501 aaacgcgatt tcgggcgctt ggtcaacgaa gccatcgctg ctgcggaggc caaattccag
     1561 gcgctcaaat ccggcttgga tgctgccgtc gaacaaatca gcgccgcctc gctcgatctc
     1621 agcgcgcccg gcgaacccgg catttggggc acccgccacc ccatttccat caccatgaac
     1681 cgcatcattc gcatcttcga acgcatcggg tttgtggtag ccgaagaccg cgaaattgaa
     1741 gacgactggc acaatttcac cgccctcaat acgcccgaag accatcccgc ccgcgacatg
     1801 caggacacct actacctgcg cggcagcgat aatctgctcc tccgcacgca cacctcttcg
     1861 gtgcaggccc gcctcatgaa cgtgcagcag ccgcctatcc gcatcattgc gccgggtcgc
     1921 gtatatcgca acgaaaccat ctcggcccgc tcgcattgcc agttccatca ggtggaaggc
     1981 ttgtatgtgg atgagggcgt atcttttgcc gatttgaagc aaacgctgct ttattttgcc
     2041 aaagaaatgt tcggcgccac gcaaattcgc ctgcgcccct cgttcttccc cttcaccgag
     2101 ccaagcgccg aaatggacgt atatctcggt accgatacgg aagacaatta ccgcctcacc
     2161 aaaggtaccg gctggctcga aatcctcggc tgcggcatgg tggaccccgc cgtgctcgac
     2221 aactgcggca ttgattccga gcgctatacc ggcttcgctt tcggcatggg catagagcgg
     2281 caggccatgc gtttgttcgg catcaacgac atccgtttgt tgtttgaaaa cgacacgcgc
     2341 ttcctccggc aattcgccac cgtagtttaa tcaatctatt ttttcatcta ctgtgaactg
     2401 cgggaaaacc aaacctgcct ccggtaggca ggctcgcagc tcgcagctca tagctcgcag
     2461 ctaaaatgaa accctccata cccaaaggca cccgcgattt cggccccgaa caggtttcta
     2521 aacgaaacta tatgttcggc atcatccgcg aagtattcat tcgctatggc taccaaccca
     2581 tcgaaacgcc cacgatggag ctgctctcca ccctcaccgg caagtacgga gaagagggcg
     2641 accgcctgct gttcaaagta cttaacaacg gcgacttcct gagcaaggcc gacgaagagg
     2701 cgctccaaaa ccgcgactcg cgcaaactca cggcctctat tgcagaaaag ggcttgcgct
     2761 acgatctcac cgtgcccttt gcccgctatg tggtgatgca ccaaaatgaa ctgagctttc
     2821 cgttcaagcg ctaccagatt cagcccgtat ggcgcggcga ccgcccgcag cgcggccgtt
     2881 atcaggagtt ttaccaatgc gatgtggacg tggtaggctc caattcgctg atgtatgagg
     2941 ccgagttggt gcaga
//
LOCUS       DAORLF010000529         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18359_length_2948_cov_2.113377, whole genome shotgun sequence.
ACCESSION   DAORLF010000529 DAORLF010000000
VERSION     DAORLF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18359_length_2948_cov_2.113377"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1912
                     /gene="topA"
                     /locus_tag="PK198_25320"
     CDS             47..1912
                     /gene="topA"
                     /locus_tag="PK198_25320"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRF42148.1"
                     /translation="MSKNLLIVESPAKAKTIEKFLGKDFTVKSSFGHVRDLEKGDGAV
                     EVENNFRPRYVVTPDKTKVVRELREWVKKVDEVWLATDEDREGEAISWHLCEVLGLDE
                     RQTKRIVFREITKPAIQAAIQKPRALDINLVNAQQARRVLDRLVGYDLSGILWKKIKG
                     GLSAGRVQSVAVKLIVEREREINEFQTTPFFKVEAIFDVPNGQGKTVALEAESPVRYD
                     AENDARTFLEKCNGAAFRISDIEVKPVKRSPAAPFTTSTLQQEASRKLGFSVNRTMSV
                     AQRLYEAGHITYMRTDSINLSETAIAAIAQVVENQYGAQYSQTRRFKNKVANAQEAHE
                     AIRPTYAEKQNVSGDRDEQRLYELIWKRTIASQMADALLEKTIVSIGISTIPDGSLQA
                     VGEVLKFDGFLKVYLEGKDEEEEETKGMLPPLKVSQMLDLNQMTATQRYTKPPARYSE
                     AALVKKLEELGIGRPSTYAPTISKIMEEGRGYVVKESREGVQRAYTVLTLQKGAIRSE
                     SRTENTGAVKNVLFPTDMGITVIEFLDEHFKEVMNYRFTAGIEQQFDDIASTGRDWTA
                     MMRDFYMPFQKLVEKTAENAERGNASSAKIPPAAAPCSPACRAGGRSSRSARPKN"
     gene            1864..2610
                     /locus_tag="PK198_25325"
     CDS             1864..2610
                     /locus_tag="PK198_25325"
                     /inference="COORDINATES: protein motif:HMM:NF024761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRF42149.1"
                     /translation="MSRRGPVIQIGTPEELGKDEKPRYANLKPGQSLDTISYEEALKL
                     FQLPLTLGPYKGEELIVGAGRFGPYVKFGEKFVSLPRGEDPMEVTLERAIEIVEAKLK
                     EDAPVAMYEGQPVTKGKGRFGPFIKWNDMFINVTKKYDFDRLSEADVKELIEAKLDKE
                     SNRFIQRWPDEGIDVENGRWGPFIRFGKSFVKIPGADGKKATLEDAAALTLEQAKALI
                     IAEVPGAFDAKKKAPAKKAAAKSTSKKTTK"
     gene            2617..2931
                     /locus_tag="PK198_25330"
     CDS             2617..2931
                     /locus_tag="PK198_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRF42150.1"
                     /translation="MNDPKQPAPGQLNIELPEEIAEGEYSNLAIISHSHSEFVVDFIR
                     LVPNVPKAKVKSRIILTPGHAKRLLKALADNVKKYEAQYGPIEDSDAPPFPPMICGRA
                     IS"
BASE COUNT          800 a          864 c          778 g          506 t
ORIGIN      
        1 aaaaaaacat acctttgcgg ttccaaagaa caaatgtagc tgactcatgt ctaaaaattt
       61 attaatagta gaatcgccgg caaaagctaa aaccatcgaa aagtttcttg gcaaagactt
      121 cactgtaaag tccagtttcg gccatgtacg cgatttggaa aaaggcgacg gcgccgtaga
      181 agtggaaaac aacttccgcc cgcgatacgt cgttacaccc gataaaacga aggtagtcag
      241 ggaattgcgc gagtgggtca agaaggtgga cgaggtatgg ctcgcaacgg acgaagaccg
      301 cgagggcgag gccatctcct ggcacctgtg cgaggttttg ggcttggacg agcgccaaac
      361 caaacgcatc gttttccgcg aaatcaccaa accggccatt caggcggcta tccaaaaacc
      421 ccgcgcactc gacatcaact tagtcaatgc gcagcaggcc cgccgcgtcc tcgaccgctt
      481 ggtgggttac gatctttccg gcattttgtg gaaaaaaatc aaaggcggcc tctccgccgg
      541 cagggttcag tcggtagccg tgaaactgat tgtagagcgc gagcgcgaaa tcaacgagtt
      601 tcagaccacg ccgtttttca aggtagaagc catcttcgac gtacccaacg gccagggcaa
      661 aacggtggct ctggaagccg aatcgcccgt tcgctacgat gccgaaaacg acgcgcgcac
      721 attcctggaa aaatgcaacg gcgccgcctt ccgcatttcc gacatcgagg tgaagcccgt
      781 caaacgcagc cccgccgcgc ctttcactac ttctaccctg caacaggaag ccagccgcaa
      841 actcggcttt tcggtcaacc gcaccatgtc ggtggcgcag cgcctctacg aagccggcca
      901 cattacttat atgcgtaccg actccatcaa cctcagcgaa accgccatcg cggccatcgc
      961 gcaggtagtt gaaaaccagt atggcgcgca gtattcccaa acccgccgct tcaaaaacaa
     1021 agtggccaac gctcaggaag cgcacgaggc catccgcccc acttatgctg aaaaacaaaa
     1081 cgtatcgggc gaccgcgacg agcagcgcct gtacgaactg atctggaagc gcaccatcgc
     1141 ctcccaaatg gcagatgcat tgttggaaaa aaccatcgtc agcatcggca tttccaccat
     1201 acccgacggc agcttgcagg cagtgggcga agtgctcaaa ttcgacggct tcctgaaagt
     1261 ttacctcgaa ggcaaagacg aggaagagga agaaaccaag ggcatgttgc cgccgctcaa
     1321 ggtgtcgcaa atgctggatt tgaaccaaat gacggccacg cagcgctaca ccaagccgcc
     1381 ggcgcgctac tcggaagccg cattggtaaa aaaattggag gaactcggca tcggccgtcc
     1441 ctccacctac gcgcccacca tctccaaaat catggaagaa ggtcgcggtt atgtggtcaa
     1501 agaaagtcgc gagggcgtac aacgcgccta cacagtgctc actttgcaaa aaggagccat
     1561 ccgcagcgag agccgcaccg aaaataccgg cgccgtgaaa aacgtgctct ttcccaccga
     1621 tatgggcatc acggtcatcg aatttttgga cgagcacttc aaggaggtga tgaactatcg
     1681 cttcacggcg ggcatcgaac agcaattcga cgacattgcc agcaccggca gagactggac
     1741 ggccatgatg cgcgattttt atatgccgtt ccaaaaattg gtggaaaaaa ccgccgaaaa
     1801 tgccgaacgc gggaacgcat cctcggcaaa gatcccgcca gcggccgcac cctgctcacc
     1861 cgcatgtcgc gcagggggcc ggtcatccag atcggcacgc ccgaagaatt aggcaaagac
     1921 gaaaaaccgc gctacgccaa cctcaagccc ggccagtcat tggacaccat cagctacgag
     1981 gaggcgctca aactcttcca actgccgctc accctggggc cgtacaaagg cgaagaactc
     2041 attgtgggcg cgggcaggtt tgggccttac gtcaagtttg gcgagaagtt cgtctctctg
     2101 ccccgcggcg aagacccgat ggaggtaacg ctggaacgcg ccatcgaaat cgtagaggcc
     2161 aagctcaaag aagacgcgcc ggtagccatg tacgaagggc agccggtgac caaaggcaag
     2221 ggcaggttcg ggccgttcat caagtggaac gacatgttca tcaacgtgac gaaaaaatac
     2281 gatttcgacc gcctgagcga agccgatgtg aaggagttga tcgaggctaa attggacaaa
     2341 gaatccaacc gcttcataca gcgctggccc gacgaaggca ttgacgtgga aaatggccgc
     2401 tggggaccgt ttatccgatt cggcaagtct tttgtaaaaa tccctggcgc cgacggcaaa
     2461 aaagccaccc tcgaagatgc cgccgccctg acgctggaac aagccaaagc gctcatcatt
     2521 gcagaagtgc ccggcgcatt cgacgccaaa aagaaggctc cggccaaaaa agccgcagct
     2581 aaatcaacgt ctaaaaaaac aacaaaataa gtaatcatga acgatccgaa acaacctgct
     2641 cccggccaac tcaacattga gttgccggaa gaaatcgccg aaggcgaata ctccaacctc
     2701 gccattattt cgcactcgca ctcagagttt gtggtggatt tcatccgctt ggtgcccaac
     2761 gtgcccaaag ccaaagtgaa atcgcgcatc atcctcacgc cgggccatgc caagcgcctg
     2821 ctcaaagcgc tggccgacaa tgtgaaaaaa tacgaggcac agtacggccc catcgaggat
     2881 tccgacgcgc cgccgttccc gccgatgatc tgcggccgcg ctatctcatg agcccgcgcg
     2941 ggcgggtg
//
LOCUS       DAORLF010000530         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18396_length_2944_cov_1.757702, whole genome shotgun sequence.
ACCESSION   DAORLF010000530 DAORLF010000000
VERSION     DAORLF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18396_length_2944_cov_1.757702"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK198_25335"
     CDS             complement(<1..242)
                     /locus_tag="PK198_25335"
                     /inference="COORDINATES: protein motif:HMM:NF040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42151.1"
                     /translation="MKSSLQQLLAEGKTEQLLSELLRLTASDPHLHEQVLLVAGRQAE
                     LERKTIAGTTSADDLDIERNKIRAALLDVVKKLPEEG"
     gene            complement(444..1097)
                     /locus_tag="PK198_25340"
     CDS             complement(444..1097)
                     /locus_tag="PK198_25340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF42152.1"
                     /translation="MYKLEADSVMLDLGGRRVLQDVYICCETGHICGILGRNGSGKSS
                     LLRIIYGDLRATDQSVRIDGEALLDRFRRPEEMRYLPQHHFIPKSLSLKAVLDDFELE
                     PADFFRAFPGFDKHYNSSMQLLSGGERRVLELYAVLMSRTRFCLLDEPFLHIMPLHVD
                     TFKELLIQEKANKGILLTDHQYRHVTELSDVLYLISQGKTHRVSGVGDLERLGYLGM"
     gene            complement(1090..1407)
                     /locus_tag="PK198_25345"
     CDS             complement(1090..1407)
                     /locus_tag="PK198_25345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42153.1"
                     /translation="MKSYGRLLTFYRSFAMPNVLLTAVCLFSMMLRGKEAIALFPGFF
                     WLKVFMLGISFYYVHTFRKKEYFYYHNLGWSKSRLWWMALGFDFGLFLIASLSLSIIF
                     RNV"
     assembly_gap    1478..1487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1492..>1729)
                     /locus_tag="PK198_25350"
     CDS             complement(1492..>1729)
                     /locus_tag="PK198_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRF42154.1"
                     /translation="TNALFAYVLSGLIAKTMFSIKWSIGPEETQTLQGWVYQNWFQSI
                     FSDYNASLAYAIANVLLVLGVTWILYSRKVFIRL"
     assembly_gap    1730..1808
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1809..>2025)
                     /locus_tag="PK198_25355"
     CDS             complement(<1809..>2025)
                     /locus_tag="PK198_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRF42155.1"
                     /translation="PEGLLSTLPAVVTGISGMLTGEWIKTSRNHYEKLTGIFGVGVLL
                     LVVSLTWDLAFPINKKIWTSSYVLYTSG"
     assembly_gap    2026..2089
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2090..2603)
                     /locus_tag="PK198_25360"
     CDS             complement(<2090..2603)
                     /locus_tag="PK198_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRF42156.1"
                     /translation="MDTSKRLLALDVFRGMTIAGMILVNNPGDWGTVYAPLLHADWHG
                     CTPTDWVFPFFLFIVGVAIPLALGRRKEAGQDRAAIFKKILFRTVAIFVIGLLLHLIP
                     KFNFEYMRIPGVLQRIALVYGACATIFMFSDWKRQLWIGAGLLVLYWALMALVPVPGG
                     YPPNLDAETNL"
BASE COUNT          676 a          773 c          754 g          588 t
ORIGIN      
        1 ccctcctccg gcaatttttt caccacatcc agcagcgccg cgcgtatttt gttgcgttcg
       61 atgtcgagat cgtcggcgga ggtcgtgccg gcaatggttt tgcgttcgag ttcggcttgc
      121 cggccggcta cgaggaggac ttgctcgtgc aggtgggggt cggaggcagt caggcggagc
      181 aattctgaga ggagttgttc ggttttgcct tcggcgagca gttgttgtag ggaggatttc
      241 atcggtgcgg atgttggtat tttgggggtg aaggtaggga attatcggca tttcaggctt
      301 agtgaagggg tgactacaat cttgttcccg gcggagtcac cccttcacta cgctgtgctg
      361 cctgccaagt cagaaagtaa ccgtcggcac gggcctgata ctccgtgaat ctctccgtgc
      421 ccgctgtgtc ctccgtggtg attttacatt ccaagatacc ccaaccgctc caaatcccct
      481 accccgctca cccgatgcgt tttgccctgg ctgatgagat agagcacgtc gctcaattcg
      541 gtcacatgcc ggtattggtg gtcggtgagg aggatgcctt tattggcttt ttcctgaatc
      601 aggagttcct taaaagtgtc aacatgcagg ggcatgatat gcaaaaacgg ctcgtccaac
      661 aggcagaagc gcgtgcgcga catcaaaacg gcgtacaatt ccaacacccg gcgctcgccg
      721 ccggaaagca actgcatgga ggagttgtaa tgcttgtcaa aaccgggaaa tgcgcgaaaa
      781 aaatcagccg gctccaattc aaaatcatcc agcactgctt tcaggctcag gcttttagga
      841 ataaaatgat gctgcggcag atacctcatt tcctccgggc gacggaaccg gtccaagagt
      901 gcctcgccgt ctattctcac cgactgatcg gtggcgcgca gatcgccgta aatgatgcgc
      961 aggagggagg attttcccga accattgcgc cccagtatgc cgcagatatg gccggtttcg
     1021 cagcaaatgt acacatcctg tagcacccgc cgaccgccca gatcgagcat cacgctgtcc
     1081 gcttcgagct tatacattgc gaaaaataat tgataatgaa agacttgcaa tcaaaaacag
     1141 cccaaaatcg aagccgagcg ccatccacca caggcggctc ttcgaccagc ccagattgtg
     1201 gtagtagaag tattcttttt tcctgaatgt gtggacgtag taaaacgaga tgccaagcat
     1261 aaagactttc agccagaaaa atcctggaaa cagcgcaatg gcctccttgc ccctcaacat
     1321 cattgagaac aggcagactg ccgtgagcaa cacattcggc attgcaaacg aacggtaaaa
     1381 cgtcaacaat ctaccatacg acttcatagc atttattttc ctgcatcgct accgcccacc
     1441 gcataccgcc caccgatccc ccggctcaaa gcacggcnnn nnnnnnngga ttcacaacct
     1501 gataaacacc ttcctgctgt acaaaatcca cgttaccccc agcaccagca agacattcgc
     1561 aatggcgtaa gccaacgagg cattgtaatc ggagaagata ctttgaaacc agttttgata
     1621 tacccagccc tgcaaagtct gcgtctcttc cgggccgatg ctccatttga tcgaaaacat
     1681 cgttttggct atcagccccg acagcacata cgcaaacaac gcgttggtgn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnncc cggaggtgta gagcacatac gagctggtcc agattttttt gttgatggga
     1861 aaagccaaat cccaggtgag actgacgacc aacaacaaca cgccgacgcc gaagatgccg
     1921 gtcagttttt cgtagtgatt tcggctcgtt ttgatccact cgccggtgag catgccgctg
     1981 atgccggtga cgacggcggg cagggtgctg agcaggcctt ccgggnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagattggtc
     2101 tcggcatcca agttgggcgg atagccgccg ggcacgggca ccagggccat cagcgcccaa
     2161 tagagcacga gcaggccggc cccgatccag agttgccgct tccagtcgct gaacataaaa
     2221 atagtagcgc aggcgccata caccagggca atgcgttgca acacgcccgg tatgcgcatg
     2281 tactcgaagt tgaatttcgg gatgaggtgc aacagcagcc cgatcacaaa aatggccact
     2341 gtgcgaaaga gtattttttt aaaaatagcc gcccggtcct gcccggcttc cttgcggcgg
     2401 cccagcgcca gcggaatggc tacgcctacg atgaacagga aaaacggaaa cacccagtcg
     2461 gtgggcgtgc agccgtgcca gtcggcgtgc agcagcgggg catacaccgt tccccagtcg
     2521 ccgggattgt ttactaagat cattccggcg atggtcatgc cgcgaaaaac gtcgagtgcc
     2581 aacaggcgtt tggaggtatc catacaggca agtgttttaa tttggcctta aatgtatgga
     2641 gtttttgaaa atagggagag aatttccaac ccggaaccag tgggcggtag gcggtattcg
     2701 gtgtgcggtg ctaagcgcgg cttgcagctt gtagccagca gccgtcccta cccgaaatct
     2761 tgagcccgaa accagtaggc ggtgaccgcg ccgtggcgcg gagtgcaaag cgtagagtgt
     2821 gcaacttacg gcttgcggcc atcggccgtc ccaacccgaa acccgaaacc ttgaacccga
     2881 aacgcgaacc ccgttcctgc cggaacgtca aaatctttaa cgcgaatcaa cgcgaaacac
     2941 gcga
//
LOCUS       DAORLF010000531         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18419_length_2941_cov_1.658351, whole genome shotgun sequence.
ACCESSION   DAORLF010000531 DAORLF010000000
VERSION     DAORLF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18419_length_2941_cov_1.658351"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>194
                     /locus_tag="PK198_25365"
     CDS             <1..>194
                     /locus_tag="PK198_25365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42157.1"
                     /translation="EAFVDFYRWNSESWLERFEYKEAGHQLLLMAFLQRIINGGGRIE
                     REMAIGNGRTDLAVFWKDQV"
     assembly_gap    195..432
                     /estimated_length=238
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(435..>786)
                     /locus_tag="PK198_25370"
     CDS             complement(435..>786)
                     /locus_tag="PK198_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42158.1"
                     /translation="TLVVTDANGCQYSVGGPMLVPNITSSTEQEAAAYTLLAFSNPTA
                     GPLALTWDLPTSRSLGMFLVSAEGRSWRLANKLPARSGNHRLDLSEWPAGTYLLRMSD
                     GLQSWTVKVVRVEL"
     assembly_gap    787..802
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<803..>1673)
                     /locus_tag="PK198_25375"
     CDS             complement(<803..>1673)
                     /locus_tag="PK198_25375"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF42159.1"
                     /translation="PASGSVRLLPDADFMNALSVYRGNCDQMTEVFCVRNPNLCEGGP
                     VVSSLTPGQTYFVRVSSTANISGYAERGNICLSIEDASLPASYTPMSLTVGVECFGNG
                     YASLNINVSGGISSYFLQGNQEHELLAAGDEYIVIATDVKGCERSVSGRIACQADPNC
                     QLTAQVEVTNTACPGGSDGSAVAVNIEGGSGSYTYEWSTGATTGAAVDLSAGWYSLTL
                     NDQNGCSIALPVRVLSPAPATLISSEIEPASGNQANGRIAVQVAGGTAPFTYDWYRNN
                     AFFANTSAPELSNA"
     assembly_gap    1674..1814
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1815..>2941)
                     /locus_tag="PK198_25380"
     CDS             complement(<1815..>2941)
                     /locus_tag="PK198_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42160.1"
                     /translation="TPAFTGRLYGPEMDGSVASSLILDFDVIYRTYTDNESFSVGVID
                     TETGQSRIAAVFTNDLGGPQLNGYVHRSVDLSAFRSKNMRFFFQYDDGGAWNWWVGVD
                     NVKLSGEGEINEVCEKARPISLDAPCVEAHNGQAIFDGPQPDCSLRNTHSLWYSYTAE
                     AAGQVKISTQSKFNDVVTVFEGPCEALLPAHCINKDEHGFTGENLYIPVEAGQTYRFR
                     VSGQTGGFGASAGSFCIGVASSNGAPAAPPHARCEQAAVLAIDADCEQTLNVHAPTAA
                     PLPSRNTLARADAWFRFTAEETTPLLIAAGADFADVITLYANGCSNLYEVAVNEYGGQ
                     LTLDEPEPGRTYWMRISGAFATIEGGVCPSVKKTVIAAPAN"
BASE COUNT          525 a          748 c          763 g          510 t
ORIGIN      
        1 ggaggctttt gtggattttt accgctggaa ttccgaatcc tggctcgaac gctttgagta
       61 caaagaagcg ggtcaccaat tactccttat ggcttttttg caacgcatca tcaatggcgg
      121 cgggcgcata gaacgcgaaa tggccatcgg caacgggcgc accgacttag ccgttttttg
      181 gaaagatcag gtcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnccctacaa ctccaccctc accactttca ccgtccagct ttgcaggccg
      481 tcgctcatgc gcaggagata agtgcccgca ggccactccg agaggtctaa gcgatgattg
      541 ccggagcgag ccggcagctt gtttgccaga cgccaactgc gcccttcggc agacactaag
      601 aacatcccca ggctgcgcga agtcggcaag tcccaagtga gcgccagcgg cccggccgtc
      661 ggattggaaa acgccaaaag ggtatatgcc gccgcctctt gttcggtcga ggaagtaata
      721 ttaggcacca gcatcgggcc gcccacgctg tattggcagc cattggcgtc ggtgacgacc
      781 aatgtgnnnn nnnnnnnnnn nncgcgttgc tcagttcggg cgcggaagta ttggcaaaaa
      841 acgcattgtt gcggtaccag tcgtaagtga acggcgctgt accgccggcc acctgcacgg
      901 caatgcgccc gttggcctga ttgcccgaag ccggctctat ctcgctgctt atcagggtgg
      961 ccggcgccgg cgacaaaacc ctcaccggca acgcgatgct gcatccgttc tgatcgttga
     1021 gcgtgagcga ataccaacct gccgacaaat caaccgccgc gccggtagtg gctccggtgg
     1081 accattcata agtataggaa ccactgccgc cttcaatgtt tacggctacc gccgagccgt
     1141 ctgagccgcc gggacatgcg gtgttcgtga cctccacctg tgccgtgagt tggcagttcg
     1201 gatcggcctg gcaggctatg cggccgctga cggaacgctc gcagcccttg acatccgtgg
     1261 ctatgacgat atattcatcg cccgcagcca gaagttcatg ttcctgattg ccctgaagaa
     1321 agtaagaact gatgccgccg ctcacgttga tgttcagcga ggcatagccg ttgccgaaac
     1381 actccacgcc gactgtcagg ctcataggtg tgtaggaggc gggcagggag gcgtcctcga
     1441 tggagagaca gatgttgccg cgctcggcat aacccgaaat attggccgta gagctgaccc
     1501 gcacgaagta ggtctggccc ggcgtgaggc tgctcacaac ggggccgcct tcgcagagat
     1561 tgggattgcg cacacagaac acctccgtca tctgatcaca gttgccccgg taaacggaga
     1621 gcgcattcat aaaatcggca tcgggtagca aacgcaccga ccccgaagca ggcnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnncgttgg caggcgccgc aatgacggtc tttttgacgc tgggacaaac
     1861 gccgccctct atggtggcaa atgcgccgct gatgcgcatc caataagtgc ggcccggttc
     1921 cggctcgtcc aaggtcaatt ggccgccgta ttcgttcacg gctacctcgt ataaattgct
     1981 gcatccattg gcatacagcg taataacgtc tgcaaaatca gcgccggcgg caatgagcaa
     2041 gggcgtcgtt tcttctgccg tgaaccggaa ccaggcatcg gcgcgggcca gggtattgcg
     2101 gctgggcagg ggcgcggcgg tgggggcgtg tacgttgaga gtttgctcgc aatcagcatc
     2161 tatggccaaa acggcagcct gctcgcaacg cgcgtgcgga ggcgctgcgg gcgcgccatt
     2221 gctggatgct acgccgatgc agaagctacc cgcagaggcg ccgaatcctc ccgtctggcc
     2281 gctgacgcgg aagcggtacg tctgcccggc ctcgacaggg atgtacaggt tttcgccggt
     2341 gaagccgtgc tcgtctttgt tgatgcaatg agcgggcagc agcgcctcgc atggcccctc
     2401 aaatacggtg acgacgtcat taaatttgga ttgggtcgaa attttgactt gcccggcagc
     2461 ttccgccgtg tagctgtacc acagcgaatg cgtatttctc aggctgcaat cgggttgcgg
     2521 gccgtcgaaa atggcctggc cattgtgcgc ttccacgcag ggcgcgtcca gcgagatggg
     2581 gcgggctttt tcgcatacct cgttgatttc gccctcgccg ctgagtttga cattatcaac
     2641 gcccacccac cagttccatg cgccgccgtc gtcgtattga aagaaaaacc gcatgttttt
     2701 ggaacgaaaa gccgacaagt ccaccgagcg atgcacatat ccgttgagct gcgggccgcc
     2761 gaggtcgttg gtaaacacgg ctgcgatgcg cgattggccg gtttccgtgt caatgacccc
     2821 gaccgaaaag gactcgttgt cggtgtaggt ccggtagatc acatcgaaat ccagtatgag
     2881 agacgaagcc acgctgccgt ccatttcggg gccatacagc cggcccgtga aagccggcgt
     2941 a
//
LOCUS       DAORLF010000532         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18468_length_2933_cov_3.703614, whole genome shotgun sequence.
ACCESSION   DAORLF010000532 DAORLF010000000
VERSION     DAORLF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18468_length_2933_cov_3.703614"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>211)
                     /locus_tag="PK198_25385"
     CDS             complement(<1..>211)
                     /locus_tag="PK198_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42161.1"
                     /translation="MRLISGIPPWVQMYYERLLERSGRQTVREIFPNFSMFVYGGVNF
                     EPYRAQLEALVGARIPSVETYPASEG"
     assembly_gap    212..434
                     /estimated_length=223
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<435..837)
                     /locus_tag="PK198_25390"
     CDS             complement(<435..837)
                     /locus_tag="PK198_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF42162.1"
                     /translation="MAFKSALIRPLARLVARRIDAWAGNAVAAQNKMLTQLLRKGAGT
                     AFGRDHGLASVRNHQDFQSAVPIRDYEQLKPYIERIKDGQRDVLWPGRPRYFAKTSGT
                     TSGVKYIPLTTDSLPNHFGTARNALFNYYARS"
     gene            complement(860..1294)
                     /locus_tag="PK198_25395"
     CDS             complement(860..1294)
                     /locus_tag="PK198_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42163.1"
                     /translation="MQRVSSNITLVLMVFVPIVWLTFFGALTITLWYYNLDYYGAIPG
                     LALRLGLLLFMAASLLFFYLTCWRLKRVETDEHFIYVTNYIKHVRYPVHQVASVKKRN
                     WGLFHTFTIRLKTPGSFGPKVTFIPSGRFFDEYIARHPEWTE"
     assembly_gap    1347..1478
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1479..2152)
                     /locus_tag="PK198_25400"
     CDS             complement(<1479..2152)
                     /locus_tag="PK198_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRF42164.1"
                     /translation="MATQRGNEAGQESREMSFLDHLEELRMHIMRSLMAITVAAIVIF
                     IFKDWVFETVIFGPSRKDFISYKVFCAISQWLGAGKALCMEPPAFKMQAVGFGESFVT
                     TLRVAFMGGIVVGFPFIINEFWKFIRPGLYDNERKAAGGMVFICSVLFLIGYFILAPF
                     ATNFLIGYTIPGVENVPTLISTLNYMIMFILPAGLVFELPVVVYFLSRIGLVTPNDMR
                     QYRRHA"
BASE COUNT          702 a          649 c          628 g          599 t
ORIGIN      
        1 agccctccga agcagggtag gtctctacgc tgggaatgcg ggcgcccacg agcgcctcca
       61 attgtgcgcg gtagggttca aaattgacgc cgccatacac gaacatggaa aaattgggga
      121 aaatctcccg caccgtttgg cggccgctgc gctccagcag acgctcgtag tacatctgca
      181 cccagggcgg aataccgctg atgaggcgca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnncgctgc gggcgtagta gttgaagagg gcattgcggg ccgtgccgaa
      481 gtgattgggc aggctgtcgg tggtgagagg gatgtatttg acgccggagg tagtgccgct
      541 ggttttggca aagtagcggg gacggcccgg ccaaagcacg tcgcgttggc cgtctttgat
      601 gcgttcgatg taaggcttga gttgctcgta gtcgcggatg ggtaccgccg attggaaatc
      661 ctgatggttg cgaacggagg ctaagccatg atcgcgcccg aaagcagtgc ccgcgccttt
      721 gcgcagcagt tgggtcaaca tcttgttttg ggcagcgacg gcattgcccg cccaggcatc
      781 tatgcgacgg gcgacgagcc gggccaaagg acgaatgagc gccgatttga aagccattgt
      841 tacttttaaa tttaggttgc tactcagtcc attccggatg ccgggcgatg tattcgtcaa
      901 aaaagcgccc ggagggaatg aacgtaacct ttggaccgaa agaccccggc gttttcagcc
      961 ggatggtgaa cgtatgaaac aggccccagt tgcgcttttt caccgaggcc acctgatgta
     1021 ccggatagcg cacatgcttg atgtagttgg tcacataaat gaaatgctca tcggtttcta
     1081 cccgcttgag ccgccaacag gttaaataaa aaaacaacaa actggctgcc ataaacaaca
     1141 gcaaccccaa ccgcagcgcg aggcccggta tggcgccgta gtagtcgagg ttgtagtacc
     1201 aaagcgtaat ggtaagcgcc ccaaagaagg tgagccacac aatgggcaca aacaccatca
     1261 aaaccagcgt aatattggaa gatactcgtt gcatacaaag tttgaatgat ggcctattcc
     1321 aagccggctt cttcgtgggc ctctttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cgcgtggcgc cggtactgtc
     1501 gcatatcatt gggcgtcacc aagccaatgc gagacagaaa atacactact accggcagtt
     1561 cgaagaccag cccggccggc aggatgaaca taatcatata gttgagcgtg gaaataaggg
     1621 tgggtacgtt ttccactccc ggtatggtgt aaccaatgag aaaattggtg gcaaagggcg
     1681 ccagaatgaa atagccgatc aaaaacaaaa cggaacaaat gaacaccatt ccaccggcag
     1741 ctttgcgctc gttgtcgtac aagccggggc ggataaattt ccaaaactca ttgataatga
     1801 aaggaaagcc aactacaatg ccgcccataa acgccactct caaggtagtg acaaatgact
     1861 cgccgaagcc cacagcctgc attttgaatg ccggcggctc catacacaaa gccttgcctg
     1921 cgcccaacca ctgagagata gcgcagaaaa ctttgtacga aatgaaatcc ttgcgggaag
     1981 ggccgaatat gaccgtttca aaaacccagt ctttaaagat aaaaataaca atagccgcca
     2041 ccgtgatggc catcaacgag cgcatgatgt gcatacgcaa ctcttccagg tggtccagaa
     2101 acgacatttc tctggattcc tgccctgctt cgttgcctct ttgagtagcc atattcgccg
     2161 ataagtcatt ttttgagagg gcaaaagtaa taaaacatca gccaatatgc cgacagataa
     2221 cgggtttaca aaaatcatac cccatacgcc ggcatcattt acacatttta gacgaacata
     2281 taaaagataa ataattgttt atcaacgcgt tttttcattc aacaaccata ctgccaatca
     2341 cagaaccacc tatatttgta ctgattttca tgtttttgga ataattacat ttgcagtgtg
     2401 gaagaacctc cgtcctcgtt tttccccctt gcacgaggac attcccacac agaacagcta
     2461 tctacacaac ttttcactgc cggccgtggc cgctgttcga agcaatctga ttgttttggc
     2521 cgctaccgct ctccttttga tatacacctc gcaccgtcag gtgtgaagcc gcttttgtca
     2581 taccgggcgc cgctaagcaa caacttaagc cgaaggcaat gcccaaaacc tggcggcgca
     2641 aggtatagtc ccaaaaacgc tgttgggaaa tggccggatt gccgtttcca acagacctag
     2701 tagtggggca aaaataaatg ttcattttct ttaggccaaa ggattagata agacacttaa
     2761 aatgaaacgt ttacttatct aatcctttgg cgaaaatgat cactttattt ttttctcgcc
     2821 ccttatacca ttttgtcatt cattcaaaat caaactttat tctctcatta tctgaaaaaa
     2881 ttgaaaagta tgagacatgt atttttaccc ttgttcctgc tcgcctccgt act
//
LOCUS       DAORLF010000533         2924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18526_length_2924_cov_2.148832, whole genome shotgun sequence.
ACCESSION   DAORLF010000533 DAORLF010000000
VERSION     DAORLF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18526_length_2924_cov_2.148832"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1220)
                     /locus_tag="PK198_25405"
     CDS             complement(210..1220)
                     /locus_tag="PK198_25405"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRF42165.1"
                     /translation="MRAPRFINYLLFAGLFLLAACQSSKNDAIITVKQNGEMTVFGQP
                     AADFQTLKAMLVDSLAKMAVMPEKIDVNFVGEVGMGARQEVETIAAEAVEAAKLLKLA
                     PTLEQQAYRQQQGTDCDKEEDDRYDCARIDLMYHTVTKGEKALQDAVTAWTNQYLFSI
                     LENAEGEVKSATLDEAAKAFFKIHNDFKKEAGGPAMMAGAFEASTGSGVELNNGKYLT
                     LAISGNTYIGGAHGSPTMTLSTFEAQTGKILTWDDLVTDKAAVQALAEKKVREAKAEA
                     FNEGFAFDDVFKFTLPANYGLTQEGIFLYYVPYEIMPYAYGATEVTLSFEELGALSKI
                     RL"
     assembly_gap    1460..1629
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1843..>2924
                     /locus_tag="PK198_25410"
     CDS             1843..>2924
                     /locus_tag="PK198_25410"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF42166.1"
                     /translation="MILQEAHFARFLDEKGHKCCTICCTIPCFQVSLFHKYTILMATF
                     KLTLRTGRTNKGGKAPIYLRITKHRTPKHISLSIFVEPRHWDPDKQRLKPSAPNSSRV
                     NALLAQKVAEAEGKAVEMERENKHVRAGSIKEHLMGKDIPEFFEYAGEYIEREYLRKG
                     QISTYKRYRSTIEKLSQYCEGKKLYMDHMTVSFLKKYEDHLRHSLNNATNTITKDLKS
                     FRRIINSAIEDDLFPFDKNPFLRFKLSTEKAEKTYLTESELKAFASVPVDAGSKREVH
                     QDMFVFACYAGGIRISDLLQLRWSNLVDGTHLSFQTQKTNTTIPVKVPDKGLAIIKKY
                     RKPDSRPQDFIFPLFDAKKDYSDPVEL"
BASE COUNT          680 a          706 c          699 g          669 t
ORIGIN      
        1 tcctgcacct cggtgagcca caggtgtcgc tcggtttcca gcaaatgccc tacatggccc
       61 tgttccagca gccattcgcc ctgcactaag caggattcat ccagatgtaa ttcgacttca
      121 gcgagtggga agttcataat ccaagacaaa cagcaaaggc ggcggattcg ttgtggaaag
      181 atgaataaac ttcgcgccca ttgcagtcat caaagcctga ttttcgacag cgcccccaac
      241 tcctcaaagc tgagggtgac ctccgttgcg ccgtaggcat aaggcataat ttcgtaagga
      301 acatagtaga ggaatatccc ctcctgtgtc aggccgtaat tggcaggcag ggtgaattta
      361 aaaacatcgt cgaacgcaaa accctcgttg aaggcttctg ctttggcctc gcgcactttt
      421 ttctctgcca aggcctgtac tgcggccttg tcggtcacga gatcgtccca agtgaggatt
      481 ttgcccgtct gcgcctcgaa ggtactcagc gtcatggtcg ggctgccatg cgccccgcct
      541 atgtaagtat tgccgctgat ggcgagggtg aggtacttgc cgttgttgag ttctacgccg
      601 ctacccgtcg aggcttcaaa tgcgccggcc atcatagcag gtccgccggc ctctttttta
      661 aagtcattgt ggattttgaa aaaagccttg gccgcctcgt cgagcgtggc tgacttgacc
      721 tctccctccg cattttccaa aatagagaac aggtattggt tggtccaggc ggtgacagcg
      781 tcctgaaggg ctttttcacc cttcgtgacg gtgtggtaca tcaggtcaat ccgggcgcag
      841 tcgtagcggt cgtcttcctc cttatcgcaa tctgtaccct gctgttggcg gtatgcctgt
      901 tgctccagtg tgggcgccag cttgagcaat ttcgccgctt cgacggcttc tgctgcaatg
      961 gtttccactt cctgccgggc gcccatgccc acttcgccca cgaaattgac gtcaattttt
     1021 tccggcatga cggccatctt ggccaatgaa tccaccagca tggcctttag tgtctggaaa
     1081 tcggctgccg gttggccgaa aacggtcatt tcgccgttct gtttcacggt gatgatggcg
     1141 tcattcttgc tgctttggca agctgccaac aaaaagagac ctgcgaaaag caggtagttg
     1201 atgaagcgcg gtgctctcat tgtatgaaaa agtttaatgg tgaacgtagg catttcaaaa
     1261 tgcgaccggc attccaaaat ctggcggact tcaggatgtc atcgcttttg ccgcaaatgt
     1321 aagcattggt tttgcaaaga caataggcgc tgagaaatca ggggagggtg gatgccctgc
     1381 cgtctatgtt cgctgcctca aacgcctcca cattgctacc acccggcgaa tcgccacagg
     1441 cggcaggaaa cctggtcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnng gaggcaggat tggagcgctt cagggcagtt tttgcggggt tgaaatcttc
     1681 agccgttggc taaactttcg cattgctgag ccctgtcagg tggaaacaaa gaagccccgt
     1741 cgcgctggcg acggggcttc ttttttgtgg tccctgcagt accattaccg atgacatgga
     1801 ttgtctttga ttttttaaaa attacttgta aatcaatgat ttatgatttt acaagaggcg
     1861 cattttgccc gctttttaga cgaaaaagga cataaatgtt gtaccatttg ttgtaccata
     1921 ccctgttttc aagtatcttt gttccacaaa tataccattc ttatggctac cttcaaacta
     1981 acgcttcgca ccggaagaac caacaaagga ggaaaagcgc ccatctatct gcgcattacc
     2041 aagcatcgta ctcctaagca catctcgctg agtatttttg tagagcccag acattgggat
     2101 ccggacaaac aacgcctgaa accctcggcg ccgaattcat cccgagtcaa tgccctgttg
     2161 gctcaaaaag tagctgaagc ggaggggaaa gcggtagaga tggaaagaga aaacaagcat
     2221 gttcgggcag gctcgatcaa agagcacctc atgggaaaag atattcctga gtttttcgag
     2281 tacgccggtg aatatatcga gagagaatac ctgcgcaaag ggcagatcag cacttacaaa
     2341 aggtacaggt ccaccattga aaaattgagc cagtactgcg aaggcaaaaa actgtacatg
     2401 gatcacatga cggtatcttt tttgaaaaag tatgaagatc acctgcggca ctcgctgaac
     2461 aatgcgacca acacgatcac caaagacctc aagtcgttcc ggcgcatcat caacagcgcg
     2521 attgaagacg atctgtttcc gttcgacaaa aaccctttcc tgcggttcaa gctgtcaacg
     2581 gaaaaggcag agaaaaccta tctgacggaa tcggaactga aagcatttgc atcggtgcct
     2641 gtagatgccg gcagcaaacg ggaggtgcat caggatatgt ttgtcttcgc ctgctatgcg
     2701 ggcggaatcc gtatctccga tttgctgcag cttcgatgga gcaacttggt ggatggcacg
     2761 catttgagct ttcaaacgca aaaaacgaac accacgattc cggtcaaagt gcccgacaaa
     2821 ggtcttgcca tcatcaaaaa gtacaggaag cccgacagcc gtccacagga tttcatcttt
     2881 ccgctgttcg acgccaaaaa ggactactcc gacccggtgg agct
//
LOCUS       DAORLF010000534         2902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18714_length_2902_cov_1.454865, whole genome shotgun sequence.
ACCESSION   DAORLF010000534 DAORLF010000000
VERSION     DAORLF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18714_length_2902_cov_1.454865"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<315..>625)
                     /locus_tag="PK198_25415"
     CDS             complement(<315..>625)
                     /locus_tag="PK198_25415"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRF42167.1"
                     /translation="ARSEGIAAKPSFRLPIRKRRCLVLADSFYEWRTEADRRVPMRIF
                     MKNGDLLVMGGIWDVWYHGDYAVKSFSIITTEPNTDMALVHNRMPLILHTAEQQEQWL
                     A"
     assembly_gap    626..696
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1088..1792
                     /locus_tag="PK198_25420"
     CDS             1088..1792
                     /locus_tag="PK198_25420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42168.1"
                     /translation="MNRNCSAFCLLLLLLGAGSAAAQSTAANKEQTVNFGLQAGAIFP
                     SRLFRVRSNEAVNNGFTYSIRADVGVQFGGMATFQLGKRFELQGGLMLLMRNYEVAVS
                     DGATRPSIQLNTTIYEIPLQMAYYQPVGNRARLVVATGVNMQSLPSNLRVRDGILDVF
                     ARKRAFSVPASLTTAGLDFRNRAKGGWMVGISYCITPFPLYDTGITARFDGKDEFFVL
                     PHIGDYFCVVGRYYID"
     assembly_gap    1910..2043
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2044..2360)
                     /locus_tag="PK198_25425"
     CDS             complement(<2044..2360)
                     /locus_tag="PK198_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42169.1"
                     /translation="MEVYPDKKFTFDTASMSTYEIIFRIDTNTLIFNYFPERYEPEGV
                     MFYKGLQVKFGFNVSIKYSVKRLLSFFENDCKLFIDDYRTQTIRESLESFDKLLSYIQ
                     LNE"
BASE COUNT          626 a          649 c          623 g          699 t
ORIGIN      
        1 gtatcacctg tgcgcacttg catttcgcgc agtatgatgg cgtctttggt gcgcttgttg
       61 cctgtaatgc tgatttcgcc gatgtagcac aggctgtcgg tttgggcggt ggcgctccag
      121 caaaaaatcc agacgaaggc agatatggac aaaattcgct gcatctcaaa acaaagtagg
      181 ggttccgggt gtcgggcggt gcaattcggg gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnntgccag ccattgctct tgctgttcgg cagtgtgcaa aatcagcggc
      361 attctattgt gtaccaaggc catatctgtg ttcggctcgg tagtgatgat ggaaaaactc
      421 ttcacggcat agtctccgtg ataccacaca tcccagatgc cgcccatgac gagcaggtcg
      481 ccgtttttca taaaaatgcg catcggcacg cggcggtcgg cctcggtgcg ccactcgtaa
      541 aaactgtcgg ccagcactaa gcaacggcgc ttgcgaatgg gcaatcggaa agaaggcttg
      601 gcagcgatgc cctccgaacg ggcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatg tattgaaggc gggccggtgc
      721 ttcattcgtg atgacataag cgtgttgtgt gggcgcaata ttgaagctct cgcgcaggtt
      781 tacccccgtg tctatgtcgc cgagttgctc gtgtactttt tcttttgtgg tcgaaaatga
      841 aaatctgccg cacattttcc gctgtttatg agtttacaaa tccggatgat gccggtgtca
      901 aaggtaataa acgcaattga tacgggaaga aaagtgcaat tttgcgcgct gtacagttaa
      961 gtttctatga gtagtgaagg ggtgaatgcg gactgtaatt ctggcagagt caccccttca
     1021 ctaacctgcc caaactcact tttttatact gcgcacatca cccaaagcaa cctgaaattt
     1081 tctcaaaatg aatcgcaatt gttccgcttt ttgtcttttg ctactcttgc tcggagccgg
     1141 tagtgctgcg gcgcaaagca ctgccgccaa taaagaacag accgtcaatt tcggcctgca
     1201 agccggcgcc atctttccca gccgtctgtt tcgggtgcgc agcaatgagg cggtgaataa
     1261 tggattcact tactccatcc gcgccgatgt aggcgtacag tttggcggca tggccacgtt
     1321 tcagttgggc aagcgcttcg agttgcaggg cgggcttatg ttgttgatgc gcaattatga
     1381 ggtcgcagtg agcgacggcg ccacgcgacc ttccatccaa ctcaatacca ccatctacga
     1441 gataccgctg caaatggctt attaccagcc cgtcggcaac cgcgcccgcc ttgtagtggc
     1501 taccggtgtg aacatgcagt cgctgcccag caatctccgc gtacgcgacg gtatcctcga
     1561 tgtgttcgcc cgcaaacgcg ccttttcggt gcccgcctcg ctcaccaccg ccggattgga
     1621 tttcagaaac cgcgccaaag gcggctggat ggtcggcatc tcgtattgca tcacgccctt
     1681 cccgctctac gataccggca ttacggcccg tttcgacggc aaagatgagt tcttcgtact
     1741 gccgcatatc ggcgattact tctgtgtagt ggggaggtat tacatagatt gaggtggttt
     1801 ttaggcatcg ccattccggt cattgcgctg attgctgcca tcagcgagga ggaggaggcg
     1861 cgggtacttg gggagcagga tgatttgttt ttcagtatgt tatcagagan nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnntgttcat ttagttggat ataagataaa agtttgtcaa agctttctaa agactctcta
     2101 atagtctgag tcctgtaatc atctataaat aatttgcaat cattttcaaa aaaagaaagc
     2161 aagcgtttaa cggaatattt tattgaaaca ttgaatccaa attttacctg taacccctta
     2221 taaaacataa ctccttccgg ttcatatctt tcaggaaaat aattaaaaat aagggtatta
     2281 gtatcaattc tgaaaataat ttcgtaagtt gacattgagg cagtgtcaaa agtaaatttt
     2341 ttgtcaggat atacttccat tagaatgact tattttatga cattctttaa acaccatttc
     2401 aaggttttgc atctttagta atttgtttta actacttacg acagtgtaca ttcgagcatt
     2461 ttttctgagc ctgaatttgg tactatcccg cttgcgccca gtcttccccc cacaaaaaac
     2521 atcatcgctc gtcccgtgtc tctgaccggg acgcctatac ccctgtctcc tgacagactt
     2581 gaacggaaaa cactcctatt tacaaatccc ccaccaactg cggaggaaca agccccgtcg
     2641 catcccatcc attctacctc taaaacgccg gcatttccgg catagttgac tgcgctgctg
     2701 taaagataat gttccggtag cgctacaatg cctgccatga ctatgctacc ggctcgcgcc
     2761 cattcttccc cccgctgtac gcggcaacgg atgccgactt ttttcagatg caaaacactt
     2821 gcgataccgg caaaaacagc ttagctttgt agcaaattgg agaatcgaat gaataatagt
     2881 cgccccttag tgagttttga ct
//
LOCUS       DAORLF010000535         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18748_length_2897_cov_2.140394, whole genome shotgun sequence.
ACCESSION   DAORLF010000535 DAORLF010000000
VERSION     DAORLF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18748_length_2897_cov_2.140394"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..945)
                     /locus_tag="PK198_25430"
     CDS             complement(130..945)
                     /locus_tag="PK198_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRF42170.1"
                     /translation="MDFLDFGKLSEMATAYLPNVLGALLTLVIGFWITGRVAHLLRVA
                     MEKNKMEASVVRFLSSLVAVGLKILVLLSVARMFGIETTSFVAIFTALAFAVGTALQG
                     GLAHFASGVLILVFKPYKVGDLVTIGGGQTGTVKEIQVFNTVLQTLDNKHIIIPNAVV
                     TSNIITNISGQGIIGVEFSFPISYQTDIDKAREVILSVAKNCPYILDEPGYGVVVAQW
                     ANDSVVLASRPFCKSGDYWNTFFYMNEEVKKAFDKAGLSIPFRQMDIHLHQAG"
     gene            1046..1528
                     /locus_tag="PK198_25435"
     CDS             1046..1528
                     /locus_tag="PK198_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRF42171.1"
                     /translation="MKNSTPKYLTLDEALAKARRYCAFQERCHSEMRSKLIEWGVYGD
                     RLEEVMADLVSENFLNEERFACTFARGKFRMKSWGRNRIVQELRARRVSDYCIRKGLQ
                     EIEEAEYLAELERLLLKKQAELSEPDTFQRRAKLMRFALQRGFEQELISGALDSIQAG
                     "
     gene            1713..2135
                     /locus_tag="PK198_25440"
     CDS             1713..2135
                     /locus_tag="PK198_25440"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRF42172.1"
                     /translation="MSTQKTETNKAPQPVGAYPHARRAGNLLFLSGIGPRDPETNGIP
                     GLERSAMGNFTEFDFEAQCHSVFRNVRAVLEAAGARWEDLVDVTVFLVNMQRDFHIYN
                     RIYAEYFKDHQPCRTTVAITALPTPIAIELKCIAYLGE"
     gene            complement(2142..>2897)
                     /locus_tag="PK198_25445"
     CDS             complement(2142..>2897)
                     /locus_tag="PK198_25445"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF42173.1"
                     /translation="KAYPVLVAENLWLHKKYKSKMLKFIAQSPLNEKPGYVYSDLSFY
                     FWPEIAPRLTGTEFETYLKKTFYEPLGAFTLTYNPLRYFPHSRIVPTERDTFFRMSLL
                     HGRVHDEGAAMLGGVSGHAGLFGAANDLAKLMQMYLNKGEYGGRRYIAAAALEEFTRC
                     AFCGEGNHRGLGFDRPLAAYDPARSYTARSASQASFGHSGYTGTFTWADPESGLLLIF
                     FSNRVYPTRDNRELYNLGIRPRLHQALYDAVRE"
BASE COUNT          695 a          803 c          767 g          632 t
ORIGIN      
        1 cgaactgctg tacaaggttt tgcagacctc tgcctagcca ctcggccacg cgaccgcgat
       61 ttgggacggc aaagataaac gttttctctt tgccgcgcca aatcgggtga aaaacttttg
      121 taaaaaaaat tatccggcct ggtgcaggtg gatgtccatt tgacggaacg gaatgctcag
      181 ccccgctttg tcaaaggctt ttttaacttc ctcattcatg taaaagaagg tgttccaata
      241 gtcgccgctt ttgcaaaacg gcctcgatgc cagcaccacg gagtcattgg cccattgagc
      301 taccactacg ccgtagccgg gttcgtccaa gatataagga cagtttttgg ctaccgacaa
      361 aatgacctca cgcgctttgt caatatcggt ttggtagctg atggggaagc tgaattctac
      421 cccgatgatg ccctgcccgg aaatattggt gatgatgttg ctcgtaacga cggcattggg
      481 aatgatgatg tgtttgttgt ctaaggtttg caacaccgtg ttgaacacct gaatttcctt
      541 caccgtgccc gtttggccgc cgccgatggt cacgaggtcg cccactttgt acggtttgaa
      601 aacgaggatg agtacgccgc tggcaaaatg cgccagaccg ccttgcagtg cagtaccgac
      661 agcaaaggcc aatgcggtaa aaatggccac aaatgaggtg gtctcaatgc cgaacatgcg
      721 ggccacggaa agcagcacta aaattttgag ccccacagct accaacgaag acaaaaagcg
      781 gacgaccgag gcctccattt tgtttttctc catcgctacg cggagcaaat gcgccacacg
      841 cccggtgatc cagaacccaa tgacgagggt gagcagtgcg cccaacacat tgggcaggta
      901 ggctgtagcc atttccgata gtttgccaaa gtctaaaaag tccatgataa gccttgtgtt
      961 ttgatgagtg aatatgattt agaagccggc tgcttcattt ggtcactaag atacaaacat
     1021 taactttgtt gccgctttta gaaacatgaa aaacagtacg cccaaatatc tcacgctgga
     1081 cgaagccttg gccaaggctc gccggtactg cgcttttcag gagcgttgcc acagcgaaat
     1141 gcgcagtaaa ctcattgagt ggggcgtgta tggcgaccgg ttggaggagg taatggccga
     1201 tctggtttct gaaaattttt tgaacgaaga gcgctttgcc tgtacttttg cccggggcaa
     1261 gtttcgcatg aaaagctggg gccgcaaccg catcgtgcag gaactgcgcg cccgccgggt
     1321 gtcggattat tgcatccgaa aaggtttgca ggaaattgaa gaggccgaat atctggccga
     1381 gttggaaagg ctgctgctca aaaaacaggc agaactctcc gagccggata ccttccaacg
     1441 gcgggccaaa ctgatgcgct tcgccctgca acgaggattt gaacaggaat tgatttcggg
     1501 cgctttggat tccattcagg ccggctagtg aaggggtgac tctgtcggag tagcgcctcg
     1561 tagtcacccc ttcactacgc gcagaaactt aaaaaaaaca tagggcactt catagaacgc
     1621 tcttatttgg caatcagtaa atcagcacaa cttgtcccga tgacgcagag cctgttccga
     1681 tggacgaagg gaatcggaaa aatattacta ccatgtccac tcaaaaaaca gaaaccaaca
     1741 aagcgcctca gcccgtaggc gcatatccgc acgcacgccg ggcgggcaat ctgctgttcc
     1801 tctccggcat tggtccccgc gaccccgaaa cgaacggcat tccgggcctg gaacgcagcg
     1861 ctatgggcaa ttttacggag ttcgacttcg aggcgcagtg ccactcggtg tttcgcaatg
     1921 tgcgcgccgt gctcgaagcg gcgggcgccc gctgggaaga cttggtggac gtcaccgtct
     1981 ttttggtcaa tatgcagcgc gacttccata tctacaaccg catctacgcc gagtatttca
     2041 aagaccacca gccctgccgc accacagtag ccatcactgc gctgcccacg ccgatagcga
     2101 ttgagctgaa gtgtattgcg tatttgggag agtagaaatc ctcactcccg caccgcatca
     2161 tacagcgcct gatgcagcct cggccgtatc cccagattgt acaactcccg gttgtcgcgg
     2221 gtaggataca cccggttgga gaagaaaatc agcagcaggc cgctctccgg atcggcccag
     2281 gtgaaagtac ccgtgtagcc cgaatgcccg aaactggcct gactcgccga gcgcgccgtg
     2341 tagctgcggg ccggatcata ggccgccagg ggccggtcga atcccagtcc ccggtgattg
     2401 ccctccccgc aaaacgcaca acgcgtaaac tcctccagcg ctgccgccgc tatgtatcgc
     2461 cggccgccgt actcgccttt gttcaggtac atctgcatca gtttggccag atcgttcgcc
     2521 gcgccgaaca aacccgcgtg cccgctcacg ccgcccaaca tggccgcgcc ctcgtcgtgt
     2581 acgcgcccgt gcagtagaga cattctgaaa aaagtatccc gctcggtagg cacaatgcgg
     2641 ctgtgcggaa aatatcgcaa cgggttgtac gtcagcgtaa aagcgcccaa gggttcgtaa
     2701 aacgtctttt tcagatacgt ctcaaactcc gttcccgtca ggcgcggcgc tatctccggc
     2761 cagaaataaa acgagaggtc ggaatacaca tagcccggct tttcgttcag cggcgattgg
     2821 gcaatgaatt tcagcatctt cgacttgtac ttcttgtgca gccacaggtt ttccgccacc
     2881 aatacggggt acgcttt
//
LOCUS       DAORLF010000536         2889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18807_length_2889_cov_1.940367, whole genome shotgun sequence.
ACCESSION   DAORLF010000536 DAORLF010000000
VERSION     DAORLF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18807_length_2889_cov_1.940367"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>403)
                     /locus_tag="PK198_25450"
     CDS             complement(<1..>403)
                     /locus_tag="PK198_25450"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42174.1"
                     /translation="DTLAMPRQTGVPVSVLQLTLHQNLSLWKFHLDNAVVFQTSSEPV
                     IRLPQIFSKHSLYYEGRWFKKALQVRIGFDVRYNTPWMADYYNPVIGQFHLQQRQEAA
                     FYPATDWFISMRRGTFRAYVKWEGLERLLFDD"
     assembly_gap    404..535
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<536..1889)
                     /locus_tag="PK198_25455"
     CDS             complement(<536..1889)
                     /locus_tag="PK198_25455"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42175.1"
                     /translation="MKLLWKYGALAIFAVFATALFAQRPTGTVPTTGGPTFGGPAQAN
                     KEQKQDEVPDTVGLHLFYADRPEWERPYSDSLLDNYFHQYDPARRRKIDLLTTGNLGG
                     ATRYAAYEPVFRRALDIGFHQYDVYHIPAAEAPFYRLQKAYTNLAYYLQGEQADSYLT
                     GQFARNFAKGTHFSIDYKRLSQIGNQNQYPQQHSRQTALTAGLWTRSENGRYNGFLVF
                     ASNTSEQEDNGGLLQEPEQIPNRPLSPANAAIFLSDAQTRYSLRELSYTQHWRLGGGT
                     DSTGQTRRAFPFMHRITWLDGQYKFFDRDPALDTAFYSRFPHLLTDERGLRYFVRHRK
                     LENTVQLATSRKGRQGGSDLLEAALIHTLNWLRLEPVDTVVNNLFLTGRMVVNPRPVL
                     RFEAQAHLGLLGNAGDYRVQAGLTLDLPKTGSLYLQFLNQFYSPTLVQHRFFISQREV
                     W"
     gene            complement(1980..2597)
                     /locus_tag="PK198_25460"
     CDS             complement(1980..2597)
                     /locus_tag="PK198_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42176.1"
                     /translation="MPRSLLLLLLLAACSEPQPQQIHLSNSSFEGTPAAGSTPDGWTN
                     CGDRGESPTDIQPGFFGVSLPAAEGRTYLSMVVRETGKLEGLSQALPTPMKPGVEHSL
                     WLSLAQSDSFSSPALNLEGKLKTADFSHSVVLRLYGGMNPCERRELLAQTPVIGHREW
                     KPYLLKWQPSAAHTHLMLETFYDTTRVQSYNGHILVDHLSEISIK"
BASE COUNT          629 a          706 c          816 g          606 t
ORIGIN      
        1 gtcgtcgaac agaagccgtt ccagcccctc ccatttaacg taggcccgga aagtccctct
       61 gcgcatactg atgaaccagt cggtagccgg gtagaaggct gcttcctgcc gctgctgtag
      121 gtgaaactgc ccgatcaccg gattgtaata atcggccatc cagggggtgt tgtaacgcac
      181 atcaaagccg attctcacct gcaaggcttt cttaaaccaa cgcccttcgt aatacaaact
      241 atgcttggaa aaaatttgcg gcaagcgaat gaccggctcg gaagaggtct gaaacaccac
      301 ggcattgtct aaatgaaact tccagaggct caggttttgg tgcagggtca gttgcagcac
      361 gctcaccgga acgcccgttt ggcggggcat ggctaatgtg tcgnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccac
      541 acttcgcgct gcgaaatgaa aaagcgatgc tgcacgagcg tgggagaata gaattgattg
      601 aggaattgaa ggtacaggct gccggtttta ggcaggtcta aagtcagccc cgcctgcacg
      661 cgatagtcgc cggcgttgcc cagcaatccc aggtgtgcct gagcttcaaa acgcagtacc
      721 ggccggggat tgacgaccat gcggcccgtg aggaagaggt tgttgacgac cgtgtccacc
      781 ggttccaagc gcagccagtt gagcgtgtgt atcagtgcgg cttccagcag atcgctgccg
      841 ccctgccgcc ctttgcgcga ggtggccaac tgcaccgtat tttccaattt ccggtgacga
      901 acgaaatagc gcagcccccg ttcatcggtg agcaagtgcg ggaaccggct gtaaaacgca
      961 gtgtccaaag ccggatcgcg gtcgaaaaac ttgtactgcc cgtctaacca ggtaatgcgg
     1021 tgcataaagg ggaaagcccg ccgcgtttgc cccgtcgaat cggtgccgcc tccgaggcgc
     1081 caatgctggg tgtagctcag ttcgcggagg ctgtagcggg tttgggcatc gctgagaaaa
     1141 atggcggcat tggccggcga cagcgggcga ttgggtatct gttccggctc ctgaagcagg
     1201 ccgccgttgt cttcctgttc gctggtattg gaggcaaaca ccaaaaagcc attgtagcgg
     1261 ccattctccg agcgcgtcca caatccggcg gtcaatgcgg tttggcggct gtgctgctgc
     1321 gggtactggt tctggttgcc gatctggcta agtcgtttat aatcaatgga aaaatgcgtg
     1381 cctttggcaa agtttcgcgc aaactgtccg gtcagatagc tgtcggcctg ctcgccttgc
     1441 aggtagtacg ccaggttggt atatgccttt tgcaggcgat aaaacggcgc ttcggcggcg
     1501 ggaatatggt aaacatcgta ttggtgaaaa ccgatgtcga gcgcgcggcg aaacacaggt
     1561 tcgtaagcag cgtagcgggt agcgccgccg agattgccgg tagtgagcag gtcaattttg
     1621 cgtcggcggg cgggatcgta ctggtgaaaa tagttgtcaa gcagcgaatc ggaatacggg
     1681 cgttcccatt ccggcctgtc cgcataaaaa aggtgcaggc cgacggtatc gggcacttca
     1741 tcttgtttct gttccttatt ggcctgtgcc ggcccgccaa aggtaggccc gccggtggtg
     1801 ggaacggtgc cggtaggccg ctgtgcaaaa agtgccgtgg cgaacaccgc gaaaatggcc
     1861 aaggccccgt atttccagag taatttcatc gttgcgaaag attcggctgc aagtttcaaa
     1921 aaatccgttg aggatgcgtt ccaaagaaac gatgaaaagg ggggaaatgt atagcgcgcc
     1981 tacttgatgc tgatctccga aaggtgatcc acaaggatat gcccgttgta cgattggacg
     2041 cgagtagtat cgtagaaggt ttcgagcatc agatgcgtat gcgctgcaga aggttgccat
     2101 ttcaacaggt agggtttcca ttcgcggtgg ccgatgacag gcgtttgagc cagcaattcg
     2161 cgccgctcgc agggattcat gccgccatac aggcgaagga ctaccgagtg cgaaaagtcg
     2221 gctgttttca gcttgccctc caaattgagc gccggactgg aaaatgaatc cgactgcgcc
     2281 aacgacaacc ataaactatg ctctacccca ggtttcatcg gcgtgggcaa cgcttgtgac
     2341 aagccctcta atttgccggt ttcccgaacg accatgctca gataggtgcg cccctcggca
     2401 gcgggcagag aaacgccgaa gaatccaggc tgtatgtcgg taggcgattc gccgcggtcg
     2461 ccgcagttgg tccagccatc gggtgtactc ccggcagcag gtgttccctc gaaagaagag
     2521 ttggagagat gtatctgctg cggctgtggc tcagagcagg cggcgaggag gagaaggagg
     2581 aggagagagc gtggcatttt ttgggggtga aggtagggag gaagacatca ttttaatata
     2641 gttgtacaaa gaacgagtgc atttactcgc catcttatgg ccgggcgtaa tttttcacac
     2701 cacttttgac caaatcaaac atccgattta gccaaaagtc atttttttat ggcttatggc
     2761 caaatgaaag gttgatgggc gtactcattg gcagacaaaa agaacgcaag aactttttgg
     2821 ctcaacctca caccgcctcc atcctcctct tcctcacctc cttgcgctgg ccctctttca
     2881 gttcaaagt
//
LOCUS       DAORLF010000537         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18816_length_2888_cov_1.781504, whole genome shotgun sequence.
ACCESSION   DAORLF010000537 DAORLF010000000
VERSION     DAORLF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18816_length_2888_cov_1.781504"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..665)
                     /locus_tag="PK198_25465"
     CDS             complement(75..665)
                     /locus_tag="PK198_25465"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRF42177.1"
                     /translation="MKILVLDNYDSFTYNLVQYIRELGFEDIDVRRNDAITLDEVEHY
                     AAIVLSPGPGLPAEAGIMPELIRRYAPHKPILGVCLGQQAIGEAFGGTLENLSAVFHG
                     VDTPIEVTRSDEPLFSGLPSVFRAGRYHSWVVKKEGFPDDLEITAQDEGGAIMALRHK
                     TYNVRGVQFHPESIMTEHGRQMLANFFELCVQEAPK"
     assembly_gap    962..975
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<976..2291)
                     /locus_tag="PK198_25470"
     CDS             complement(<976..2291)
                     /locus_tag="PK198_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRF42178.1"
                     /translation="MTKTTTMTMQAPQHNAPIPLRSLVRRALADTVTPVAAFLKLRDH
                     YTCPVLLENNNFGSLEDCRSFIGLDVMASFTVENAKIRMQFPGGLTDSMPVETTEDVP
                     AQLHRFMSRFQPDYETPYAGFNGLIGYTGFDGVQHFDTLHFDPAKRKMRVPELRYHLY
                     RFVIAFHHFNDEIYIIENLLPGQESELKRLETLLWSRKISTHAFRLDGEEQSNLTDEE
                     FKELVALGKHHCRIGDVFQIVFSRQFSRGFRGDDFNVYRVLRSVNPSPYLFYADFGSF
                     RIFGSSPETQMAVADGVAKVNPIAGTYRRSGDPAEDAQKAIDLAADAKENAEHIMLVD
                     LARNDLGRHAVNVRVRDLKHIHFYSHVIHLVSKVEGDLPPGANPVQIFADTFPAGTLS
                     GAPKYKAIELIDRYENQNRGVYGGAIGLMDFNGNMNKAIIIRSFLS"
     assembly_gap    2564..2673
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          607 a          731 c          757 g          669 t
ORIGIN      
        1 gagccatatg tatgttgtct gtttaaaacc agatgcgccg ttcaatatgt atcattgaaa
       61 atcagccctt tacattattt cggagcttcc tgaacgcaca actcaaaaaa attggccagc
      121 atctgccgcc cgtgctcggt cataatggat tcggggtgaa actgcacgcc gcgcacatta
      181 taggtcttgt gtctcagggc catgatggcg ccgccctcgt cctgggcggt gatttccaga
      241 tcgtcgggga agccttcttt tttgaccacc caggaatggt agcgaccagc gcggaacacc
      301 gacggcagcc cgctgaacaa aggctcgtcg gagcgggtca cctctatggg cgtgtccact
      361 ccgtgaaaaa cggcgctcag gttttccaag gtaccgccga acgcctcgcc aatggcctgc
      421 tgccccaaac acactccgag gatgggcttg tgcggcgcat accggcgaat gagttcgggc
      481 atgatgcctg cttcggctgg cagccccggc cccggcgaca gcacgatggc ggcgtaatgc
      541 tccacttcgt ccaatgtgat ggcatcgttg cggcgcacgt caatgtcctc aaatccgagt
      601 tcacggatgt attgcaccag attgtaggta aaagaatcgt agttgtctaa aacgagtatt
      661 ttcatgattt tcagaatgtt gttttttgtt tttgaagcgt gtttttgcat caatctgtga
      721 tcttccgtcg tactgtggca cacagtttat cgtagcggct gcaatcgaat ccgtaatctg
      781 ctcagttcat cgtccgggcg gaatccgaaa tcaggaatcc gaaatgtgcc atcaccccac
      841 ccgttccgcc tccagcagcg cttttttcag ggcgcccaat ttgttgttca cctcctgcaa
      901 ttcgctttcc tcgttgctcg ccgccaccac gcccgcgccc gcctgataaa acagcgtatt
      961 gnnnnnnnnn nnnnntggct gagaaaagag cgaatgataa tggctttgtt catgttgccg
     1021 ttaaaatcca tcagcccgat ggcgccgccg taaacgcccc ggttttggtt ttcgtagcga
     1081 tcaatgagtt cgatggcctt gtatttcggg gcgccggaga gtgtgccggc gggaaaagtg
     1141 tctgcaaaaa tctgcacagg attggcgccc ggcggcaaat cgccctccac tttcgatacc
     1201 aaatggatga cgtggctgta aaaatgaatg tgcttcaaat cgcgcacccg cacattgacg
     1261 gcgtgacggc ccagatcgtt gcgggcaagg tccaccagca tgatgtgttc ggcattttct
     1321 ttggcatcgg cagccaggtc aatggctttt tgcgcgtctt ctgcggggtc gccgctgcgg
     1381 cggtaagtgc ctgcaatggg attcactttg gccaccccat cggccaccgc catttgtgtt
     1441 tcgggcgagg aaccaaagat gcgaaaactg ccgaaatcag cgtaaaacaa atacggagag
     1501 ggatttacgg agcgcagcac gcgatacaca ttgaagtcgt cgccgcgaaa accacgcgaa
     1561 aactggcggg aaaacacaat ctgaaacacg tcgccgatgc ggcaatgatg cttgcccaaa
     1621 gccaccaatt ctttaaattc ctcatctgtg agattgctct gctcctctcc gtccaagcgg
     1681 aaagcatggg tagatatttt gcggctccac agcagtgttt ccaggcgttt caactccgat
     1741 tcctgcccgg gcaacaggtt ttcgatgatg taaatctcat cgttgaaatg atgaaaagca
     1801 atgacgaagc gatacaggtg gtagcggagc tcgggcacac gcattttgcg tttggccggg
     1861 tcgaaatgga gcgtatcgaa atgctgcacg ccatcgaagc cggtgtaacc gatcaatccg
     1921 ttgaagccgg cataaggcgt ttcgtagtcc ggctggaacc gcgacatgaa gcggtgcaat
     1981 tgagccggca cgtcttcggt cgtttcaact ggcatggagt cggtcagccc accgggaaac
     2041 tgcatcctga tctttgcatt ttccactgtg aatgaagcca ttacatccaa tccgatgaac
     2101 gacctgcaat cttcgaggct gccgaaatta ttgttttcca acaacaccgg acaggtgtaa
     2161 tgatctctca atttcaggaa agccgctacg ggcgtaaccg tgtcggccaa ggcccggcgc
     2221 acaagggagc gcaagggaat gggcgcgttg tgctgtggcg cctgcatggt catggtggtt
     2281 gttttggtca tgtttaagtt gtttgaaatg cctgaaaata aaaaatcggc ttgccgggcg
     2341 aaccgacaag ccgattgatc tgatagcacg aaagctatat ggccgtcacc tcacccggtt
     2401 ttccgaatga atgtgccacc accaagcatt tgtgaacaga acagaaatca tttttttaaa
     2461 aattgatcgg gccaaaggta tgtcaatgtt tatggaacga gcaagccatt gccgaaaaat
     2521 attttatcgc agctcttccc cgctcgattt gcctttttct ctcnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactgcgc ggagtttcgg tacgcgtggg
     2701 tgcggtttct cggcgaggct cggcactgcg cggagcttcg gtacgcgtgg gcgcggtttc
     2761 tctacgcggt tcagtactgc gcggagtttc ggtacgcgtg ggcgcggttt ctcggcgagg
     2821 ctcggcactg cgcggagctt ctaccggctg ctgtgtacgc ggttcggtgg tgcgcggcgc
     2881 tgtttccc
//
LOCUS       DAORLF010000538         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18938_length_2873_cov_1.557488, whole genome shotgun sequence.
ACCESSION   DAORLF010000538 DAORLF010000000
VERSION     DAORLF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18938_length_2873_cov_1.557488"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    299..365
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <366..623
                     /locus_tag="PK198_25475"
     CDS             <366..623
                     /locus_tag="PK198_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42179.1"
                     /translation="RAKLRAEEEVEAIFAQQEAENEYYKRIAEEARVREGEERRQKEE
                     ERRHKEEAQIKLARLLLGTGMSVEEVALETGLSEEEVEKIR"
     assembly_gap    881..928
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <929..>1289
                     /locus_tag="PK198_25480"
     CDS             <929..>1289
                     /locus_tag="PK198_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRF42180.1"
                     /translation="GKALDTWQQAPIDNYSTFLRGTDDYKDTAGSDIVVITAGVPRKP
                     GMSRDDLITTNAKIVNTVTKSVMEHSPNAILIVVSNPLDVMTYAAYKASGLPASKVIG
                     MAGILDTARYRAFLAEEL"
     assembly_gap    1290..1316
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1317..1731
                     /locus_tag="PK198_25485"
     CDS             <1317..1731
                     /locus_tag="PK198_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRF42181.1"
                     /translation="LGGHGDTMVPLPRYTTVAGIPVTELLEMDKINAIVERTKSGGGE
                     LVKLMGTSAWYAPGAASAQMVEAILRDENRVFPCCIKLEGQYGMDDVFLGVPVKLGKN
                     GVEQVLELNLNEDEMTLMRNSADAVRSVMAVYDQM"
     gene            2002..>2873
                     /locus_tag="PK198_25490"
     CDS             2002..>2873
                     /locus_tag="PK198_25490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42182.1"
                     /translation="MKKHFCLLLLMLPLTLVGQTPISGVINRYAAVNNINYCSSTLSV
                     NNAAGFAPGDRVLIIQMKGAIINSSGTAAFGSITNLGAAGKYEWATVQTVQLNNIRVQ
                     FALANNYDITGNVQMVWVPRYTDAVITGQLSAPAWNGSIGGVLAMQVSGTLNFQEDID
                     LSAGGFRGGIADINAVNGCSWLIPQNGYFYPLNNWRGAAKGEGIAVFLNGAESGRGPQ
                     ANGGGGGNDHNAGGGGGGNSSAGGQGGTNNEPSTFGCSGQFPGLGGRAVALDGTHLIM
                     GGGGGAGHENNDLAS"
BASE COUNT          653 a          728 c          783 g          567 t
ORIGIN      
        1 gcgcacctgc cgctctaagg tgggcgcttc gttgagtaca ggaatggtta catcaaaggt
       61 cataattgga cgtggtttct gcgggcgaag ataggggagt ggggggattt ttccgctttt
      121 tcagacatgt caaggtttca taaatcttac catgtctggg cgtcggtctg caactttgcc
      181 tgtcgcgctc ttgtttttag ccacaatatc gtaactttgc cttatgaaaa ccgttatacg
      241 atcacgcctt taagtacctg atgtccaacg acaaactggc ccaagaggtg ccggaaggnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnagggc caaactgcgc gcagaagaag aagtggaggc catcttcgct cagcaggagg
      421 cggagaacga gtactataag cgaatagcgg aagaagcaag agtgcgtgaa ggagaagagc
      481 gcaggcaaaa agaagaagag cgcaggcaca aagaagaagc ccagatcaaa ttagcccgcc
      541 tgctgctggg cacggggatg tccgtcgaag aggtggcgct tgaaaccggg ctgtcggagg
      601 aggaggtgga gaaaattagg tgagctggat cttgcccccc ctcgcgcatt tttcgccctc
      661 cggcaagaca ggtatgacaa aaatcagccc cattcatccc catagtttcc ctacctttgc
      721 ggcgcttttg ggcgggttgc gaacataacc cggtccgggc ggttgacaag taaaattctt
      781 aacatctttt caatatgagc aaagtaactg ttatcggtgc gggcaatgtg ggggctaccg
      841 tagccaacac gctggcgcac aaagatttgg taaaagaaat nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ggcaaagccc tcgacacctg gcaacaggcg
      961 cccattgaca attacagcac cttcctgcgc ggtaccgacg actacaaaga caccgccggt
     1021 tccgacatcg tggtcatcac tgccggcgtg ccccgcaaac caggcatgag ccgcgacgac
     1081 ctcatcacta ccaatgccaa aatcgtcaat accgtgacga aatcggtgat ggaacactcg
     1141 cccaacgcca ttctcatcgt cgtttccaat cctttggacg tgatgacgta tgccgcctac
     1201 aaagcctccg ggctgccggc ttccaaagtg atcggcatgg cgggcatttt ggatacggcc
     1261 cgttaccgcg cgttcctggc tgaggagctn nnnnnnnnnn nnnnnnnnnn nnnnnngctg
     1321 ggcggccacg gcgatacgat ggtgccgctg ccccgctaca cgacggtggc cggtattccc
     1381 gtgacggaat tgttggaaat ggacaaaatc aacgccattg tggaacgcac caaatcgggc
     1441 ggcggcgaat tggtgaaact catgggcact tctgcctggt atgctccggg tgctgcatct
     1501 gctcagatgg tggaggccat tctgcgcgac gaaaatcgcg tattcccttg ctgcatcaag
     1561 ctcgaaggcc agtacggcat ggatgatgtg ttcctcggcg tgccggtgaa attgggcaaa
     1621 aacggcgtgg agcaggtact cgaattgaat ctcaacgaag acgaaatgac gctcatgcgc
     1681 aactctgccg acgcggttcg ctcggtgatg gcggtgtatg atcagatgtg attcttcgag
     1741 tttctattaa aagaagttca caatagctgc gagccgcgag ctacgagctg cgagcttgcc
     1801 ttttcgttgt aaatgagatt ttccattttg ggagagtttc aaaatatgtt gttctaaggt
     1861 gacgtctgct gctgcacggg tggtggcaga tgtcaccttt gatgtataaa aacgataacc
     1921 ccaaaggatg caaaaatatt agctctggaa gctataacag cccctatctt tgtactgcca
     1981 tccaaacaac tcaaaacagc tatgaaaaaa cacttctgct tattgctcct gatgctgccg
     2041 ctcaccctcg tcgggcagac gcccatttcc ggcgtcatca acagatatgc tgccgtgaac
     2101 aacatcaact attgctcctc caccctgagc gtgaacaatg ccgccggctt cgctccgggc
     2161 gaccgcgtgc tcatcattca gatgaagggc gccatcatca actccagtgg tacggcggct
     2221 ttcggcagca tcaccaacct cggtgcggcg ggcaaatacg agtgggccac ggtgcaaacg
     2281 gtgcaactca acaacatccg ggtgcaattt gccttagcca acaattacga cattaccggc
     2341 aatgtgcaaa tggtatgggt accccgctac accgatgccg tcatcactgg ccagctgagc
     2401 gcccctgcct ggaacggctc catcggcggc gtgctggcca tgcaggtaag cggcactttg
     2461 aactttcagg aagatattga tctgagcgcc ggcggatttc ggggcggcat tgccgatata
     2521 aatgcagtga atggctgcag ttggctcatt ccgcaaaacg gctatttcta cccgctcaac
     2581 aactggcggg gcgcggccaa aggcgagggc atagcggtct tcctgaacgg tgcagaatcg
     2641 gggcgagggc cgcaggccaa tggcggcggc ggcggcaacg atcacaatgc aggcggcggc
     2701 ggcggcggca acagcagcgc cggcgggcag ggcggaacca acaatgaacc ctctactttt
     2761 ggttgcagcg ggcagtttcc ggggctgggc ggcagagccg tcgcacttga cggaacacat
     2821 ctcatcatgg gcggcggcgg cggcgcaggc catgaaaaca acgacctcgc atc
//
LOCUS       DAORLF010000539         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_18979_length_2868_cov_1.709918, whole genome shotgun sequence.
ACCESSION   DAORLF010000539 DAORLF010000000
VERSION     DAORLF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18979_length_2868_cov_1.709918"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    311..372
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<373..1514)
                     /locus_tag="PK198_25495"
     CDS             complement(<373..1514)
                     /locus_tag="PK198_25495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF42183.1"
                     /translation="MAARSLGMLYTATYDAWAAYDDVAKPVHSTVARRPAPERSNDNF
                     RVPAINRAAYRILLDQFPSVKETILDKTLLKLGVNPTDNNVNPGNGVGVGNLVANDNI
                     NFRHSDHSNQANGYADTTSYQPVNEPMSIAFPNAFDEIKYPGRWQPLSYYDPVSEMPA
                     SPKFIAPHWGLVKPFALSSGSEFRPNPPQHLLSQGFLDQAKHVIDIQANLTTEQKVIA
                     EYWADGPKSELPPGHWALFTAYVIKRDDMNLAQSVKLFFAVANAIADAAIATWEAKVF
                     YDYVRPITAIRYLFRDKWIRGWGGPGKGIVNMLGQNWRTFQSDTFPTPPFAEFTSGHS
                     GFSMAAATVLKRFTGSDKFGYYHAQTNDLVADPTEDVKGIVMRWDT"
     assembly_gap    1556..1689
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1827..1910)
                     /locus_tag="PK198_25500"
     tRNA            complement(1827..1910)
                     /locus_tag="PK198_25500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1874..1876),aa:Leu,seq:cag)
     gene            complement(2031..2110)
                     /locus_tag="PK198_25505"
     tRNA            complement(2031..2110)
                     /locus_tag="PK198_25505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2075..2077),aa:Leu,seq:caa)
     gene            complement(2136..>2563)
                     /locus_tag="PK198_25510"
     CDS             complement(2136..>2563)
                     /locus_tag="PK198_25510"
                     /inference="COORDINATES: protein motif:HMM:NF019363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster co-chaperone HscB C-terminal
                     domain-containing protein"
                     /protein_id="HRF42184.1"
                     /translation="DFYTLEPEEKQAEILELSTINNQAYHTLADFDKRVRYILELKGH
                     WADEGQNQLPNDFLADMMDINEAIMELDFDFDEKKMQETQEAVAGLEQQLWDSVLPVF
                     QQFDQNPNDASPLPAAKDYYLKKRYLLRIKENLSKFASL"
     assembly_gap    2564..2642
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2643..2842
                     /locus_tag="PK198_25515"
     CDS             <2643..2842
                     /locus_tag="PK198_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42185.1"
                     /translation="PEKLKIIHTPMFFPKTHAGPFGSSVRSDIFFHRLKKNKRVLPFS
                     KNPPVFCYFTQLYICASQFEA"
BASE COUNT          592 a          634 c          688 g          679 t
ORIGIN      
        1 tgctaccggc ttcaagaaaa tgcacgatga gcagcaggcc gcattggtag ccgctgcgct
       61 gggggagtaa actttctaaa tgagtgcctc aacaaagccc aaatagctgt tttacagttg
      121 tttgggcttt attttagata taaaaaaatc ccgaactccg gctctcgcca aaattcggga
      181 tggttcatgc aatcgtcatt cgacgctgcg gtcagatggt gccgagccag tgtttttcgg
      241 cccggacata cactttgtca cctacttttt ggcccagatc aaggccggcc acattgccct
      301 cgtagaagtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnaacgtgtc ccaacgcatt acaatgcctt tgacatcttc tgtcggatcg
      421 gcaactaagt cattggtttg cgcgtgatag tacccaaact tgtcactgcc ggtgaagcgc
      481 ttgagaacgg tagccgctgc catactgaac ccgctgtgcc cggaggtaaa ttcggcaaag
      541 ggcggggttg ggaaggtatc gctttggaaa gtcctccaat tttggcccag catgttcacg
      601 atgcctttgc cgggtccgcc ccatccgcga atccatttgt cgcggaacag atagcgaatg
      661 gctgtgatgg gccgaacata atcataaaac accttggctt cccaggtggc aatggctgca
      721 tcggcaatgg cgttggccac agcaaaaaac agtttcaccg attgtgccag attcatgtca
      781 tcgcgtttga tgacataggc agtgaacaaa gcccagtgtc ccggaggaag ttcggattta
      841 ggtccgtcgg cccagtactc ggcaatgact ttttgctctg tcgtcaggtt ggcctgtatg
      901 tctataacat gcttagcctg atcaagaaac ccttgagaga gcaggtgctg tggcggatta
      961 ggccgaaatt cgctgcccga agacagtgcg aaaggcttca cgagacccca atgcggcgcg
     1021 atgaacttgg gcgatgccgg catttccgaa accggatcgt aataagaaag cggttgccaa
     1081 cggcccggat atttgatttc atcgaatgcg ttaggaaaag cgatgctcat cggttcgttc
     1141 acgggctggt acgaggtggt gtcggcgtac ccgttagctt ggttggaatg atcgctgtgg
     1201 cggaaattga tgttgtcgtt ggctacaaga ttacccacgc ctactccgtt gccgggattg
     1261 acgttgttat cggtagggtt gacgcccaac ttcagcagcg tcttatcgag tatggtctct
     1321 ttcacgcttg gaaactggtc gagcaggatg cggtaggcgg cccggttgat ggctggaact
     1381 ctgaagttgt cgttgctgcg ttcgggtgcg gggcgtcggg ctacggtact gtgtacgggt
     1441 ttagccacat cgtcgtaggc tgcccaggca tcgtaggtgg cggtgtacaa cattcccaaa
     1501 gagcgtgcag ccatgggtgg gccgggtttt gtttggcgga tggcctccaa tgcggnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnc attgatattg catttcggag ctatacaaat aaaatattat taatcacttt
     1741 ataaatttaa ttgatttcca gttaattgtg cagaaatgac agcaaaatac aaaaaaaagc
     1801 ccccggagaa catccggagg cttatgtgcc gaaggcgggg gtcgaacccg cactcccttg
     1861 cgggaacacg cccctgaaac gtgcgcgtct gccagtttcg ccacttcggc ggcagattgt
     1921 cttaatgaag gagtgacctg aagtcatccc ttcactaaat atactaattg aatcgtaccg
     1981 aaggtgggac ttcccgccat tgcgggatcc ccccttcact aagtgaatcg taccgaaggt
     2041 gggactcgaa cccacacacc attggtacac gattttgagt cgtgcgcgtc taccattccg
     2101 ccacttcggc aacttatcag ggtgatgccc ttcttttaga gcgacgcaaa tttagagaga
     2161 ttttctttga tgcgcaatag gtagcgtttt tttaaataat aatcttttgc agcggggagc
     2221 ggggacgcat cgttcgggtt ctggtcaaat tgttgaaaaa caggcagtac gctgtcccaa
     2281 agttgctgtt ccagccctgc gaccgcctct tgagtttcct gcattttttt ttcatcaaag
     2341 tcaaaatcga gttccataat ggcctcgttg atatccatca tgtcggccag aaaatcgttg
     2401 ggcagttgat tttggccctc gtcggcccag tggcctttga gttcgagtat ataccgaacg
     2461 cgcttgtcaa agtcggcgag ggtgtggtat gcctgattgt tgatggtgga aagttcgagt
     2521 atttctgcct gtttttcctc tggctccagc gtgtagaagt ctgnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnagcctgaa aaactcaaaa taattcatac gccgatgttt ttccccaaaa cccatgccgg
     2701 gccgtttggt tcaagcgtcc gctcagacat ttttttccat cgcctcaaaa aaaacaagcg
     2761 ggtcttgcct ttcagcaaaa acccgcctgt tttctgctac tttacgcagc tctatatatg
     2821 cgcctcgcaa tttgaggctt gaggcttaca ggccatgccc atgctctt
//
LOCUS       DAORLF010000540         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19051_length_2860_cov_2.218538, whole genome shotgun sequence.
ACCESSION   DAORLF010000540 DAORLF010000000
VERSION     DAORLF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19051_length_2860_cov_2.218538"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="PK198_25520"
     CDS             complement(<1..2056)
                     /locus_tag="PK198_25520"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HRF42186.1"
                     /translation="MRILYFLFVSLFLLTGCMKKPAPSQATLQAPGGKIRLEFMLKSD
                     GSPAYAVFLADKVLIDSSTLGFTLKDEPALRANFEVIKFSNSSFDETWETVWGERRKI
                     RNHYNELTAYLREKEAPNRLMNLIFRSYDDGVAFHYVIPEQPGIPANFVIMEEHSEFR
                     FTGDHTAWWKPADIDSYEYLYETTPLSKIDASKYAPENERVDRRILNFKAVNTPITLR
                     TAEGVHISLHEARQLDYPDMTLALKGNTDLVSELVPWADGDRVKATLPLRTPWRTITI
                     SDRAGGLVESNLILNLNDPNKLKDLSYIEPMVYTGIWWEMHIGKTTWRRDFVEGSWSN
                     MGGQVHGATTENTKRHIDFNAANGIRGLLIEGWNTGWEYWGMDTIGFFDFVTPYPDFD
                     IKEVVRYANEKGVAIIGHHETSGDVENYEKHLEKAFQLYKDLGIKAVKTGYAGEIRPK
                     GERHNGQYMIRHYQKVIELAAKYGIAINAHEPNKDTGLRRTWPNYMTREGLRGTEYEA
                     WSAGNPPEHTVILPFTRLLGGPGDYTAGIFDVLFDKYKKDERVHSTVAKQLAVMVTLY
                     SPHQMAADLPENYEGKPAFQFVRDLVADWDETIVLNAEVGDYLTTVRKAKGSPNWFLG
                     STTDENARELSASLSFLDAGKTYVADIYADGPGADWERNPFPVAIQKVLVTKDMTLPL
                     KLA"
     gene            2200..2718
                     /locus_tag="PK198_25525"
     CDS             2200..2718
                     /locus_tag="PK198_25525"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF42187.1"
                     /translation="MMFSTRFFGALVAVILSVGAMQAQKYGHLNLGNAVAMMPETKAA
                     DAELEAFQKQMVAKGEEMAKKFQENYNKLVQDAQTGTFPPAALKKREEELVKERDAIL
                     KYEQEIEAAVQKKREELLKPIFDKVEKAIDEVAKENGYQLVFDSSIFNTLLFAQDQDD
                     LMPLVKAKLGIQ"
BASE COUNT          635 a          688 c          824 g          713 t
ORIGIN      
        1 gcgccaattt cagcggcagc gtcatatctt tggtaaccaa tactttctga atggcaacgg
       61 ggaatggatt gcgctcccag tcggcgccgg gaccgtcggc gtaaatgtcg gccacatacg
      121 ttttgccggc atcgaggaag ctgagggaag cgctcagttc ccgcgcgttt tcatcggtag
      181 tgctgccgag gaaccagttg ggcgagcctt tggccttgcg cacggtggtg aggtagtcgc
      241 ccacttcggc attcagcacg attgtttcat cccaatcggc aaccagatcg cgcacaaatt
      301 ggaaagcggg tttgccctcg tagttttcgg gcaggtcggc agccatttgg tgcgggctgt
      361 acagcgtgac catgacggcc aattgtttgg ccacggtgct gtgtacgcgc tcgtcttttt
      421 tgtatttgtc gaacaacaca tcaaaaatgc cggccgtgta atcgccgggg ccgcccaaca
      481 ggcgcgtaaa cggcaaaatg accgtgtgtt cgggcggatt gccggcgctc caggcttcgt
      541 attccgtgcc gcgcaggccc tctcgggtca tgtagttggg ccaggttcgg cgcagaccgg
      601 tgtctttgtt cggctcgtgc gcattgatgg caatgccata tttggcggcc aattctatca
      661 ctttctggta gtggcgaatc atgtactgtc cgttgtggcg ctcgcctttg gggcggattt
      721 cgccggcata accggttttc accgctttga tgcccaggtc tttgtacaat tggaatgctt
      781 tttccaagtg tttttcataa ttctccacat cacccgaagt ttcgtggtgc ccgatgatgg
      841 ccacgccttt ttcattggca taacgcacca cttctttgat gtcgaaatcg ggataaggcg
      901 tcacaaaatc gaaaaagccg atggtgtcca tgccccaata ttcccagccg gtgttccagc
      961 cctcgatgag caggccgcga atgccgttgg ccgcgttgaa atcaatgtgg cgcttggtgt
     1021 tttcggtggt ggcgccatgc acctgtccgc ccatattcga ccaggaaccc tctacgaaat
     1081 cgcggcgcca ggtggttttg ccgatgtgca tttcccacca gatgccggta tagaccatcg
     1141 gttcgatgta ggagaggtct ttcaatttat tgggatcgtt gaggttgaga atcaggttgg
     1201 attccaccaa tcctcctgcg cggtcgctga tggtgatggt gcgccagggc gtgcgaaggg
     1261 gtagggtagc ttttacgcgg tcgccgtcgg cccagggtac caattcgctg acaaggtcgg
     1321 tattgccttt cagcgccagc gtcatgtcgg ggtagtccaa ttgccgggct tcgtgcaggc
     1381 tgatgtgaac gccctcggcg gtgcgcaggg tgatgggcgt gttgacggct ttgaagttga
     1441 gaatgcggcg gtcaacccgt tcattttcag gcgcatattt cgaggcgtct attttgctca
     1501 gcggcgtggt ttcatacaaa tattcgtagc tgtcaatgtc ggcgggtttc caccaggcgg
     1561 tgtggtctcc ggtgaaccgg aattcggaat gttcttccat gatgacgaaa ttggccggaa
     1621 tgcctggctg ttcggggatg acgtagtgga atgccacgcc gtcgtcgtag gaccggaaga
     1681 tgaggttcat caggcggttg ggcgcttctt tttcgcgcag ataggcggtg agttcgttgt
     1741 agtggttgcg gattttacgg cgttcgcccc acaccgtttc ccaagtttca tcaaaagagg
     1801 agttggaaaa tttgatgacc tcgaaattgg cgcgcagggc aggctcgtct ttcagcgtga
     1861 agccgagcgt ggagctgtca atcagcactt tatctgccaa aaacacagca taagcgggcg
     1921 atccgtcgga ttttagcatg aattcgagtc gaatcttgcc gccgggcgcc tgcagcgtgg
     1981 cttgtgaagg agcgggtttt ttcatacatc ccgttaaaag gaacagtgaa acgaaaagga
     2041 aatacaaaat tctcatggcg tattgatttt tgaggcggaa agttagggag tttagctcgc
     2101 taccggtgag ggggctttgt aataatcctg ccataaaaca gccccatatt acgccagagc
     2161 ggctaccttt gtcgtttagt acaaaaaaat catccattca tgatgttctc aacacgtttt
     2221 ttcggagcgc tggtagcggt tattttgtcg gtcggcgcca tgcaagctca aaagtacggc
     2281 cacctcaatt tgggcaatgc tgtggccatg atgcctgaaa ccaaagctgc cgacgcagaa
     2341 ttggaggcgt tccaaaagca gatggtggcc aaaggcgaag aaatggccaa aaaatttcag
     2401 gaaaactaca acaagctcgt gcaggacgcc caaaccggca cctttcctcc ggctgctctc
     2461 aagaagcggg aagaggaatt ggtcaaagaa cgcgacgcca tcctgaaata cgagcaggaa
     2521 attgaagccg ccgttcagaa aaagcgcgag gaactgctca agcctatttt tgataaagtg
     2581 gaaaaagcca ttgatgaagt ggccaaagaa aacggctacc aattggtgtt cgattccagc
     2641 attttcaata ccctgctttt tgctcaggac caggacgatc tgatgccctt ggtgaaggcc
     2701 aagctgggga tacagtagga ttttaaaata attgaatttg tgcaatagct gcgagttgcg
     2761 ggccacgagc tgcgagtttg gatatccttc cataaatgcg ccgttcataa ggggcgataa
     2821 aaaaataaag tgatcatttt cgccaaagga ttagataagt
//
LOCUS       DAORLF010000541         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19084_length_2856_cov_2.204213, whole genome shotgun sequence.
ACCESSION   DAORLF010000541 DAORLF010000000
VERSION     DAORLF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19084_length_2856_cov_2.204213"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..>245)
                     /locus_tag="PK198_25530"
     CDS             complement(21..>245)
                     /locus_tag="PK198_25530"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42188.1"
                     /translation="EQLIITYEAPDYEPAYQIRIFDALGRLVLSRDVRPPRFGEKTQQ
                     IDVHSWPAGVYFITLAHGEQLLTQRFVVAH"
     assembly_gap    246..318
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<319..>564)
                     /locus_tag="PK198_25535"
     CDS             complement(<319..>564)
                     /locus_tag="PK198_25535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF42189.1"
                     /translation="NEYGEFGGTSAATPHVSGAIALLYSLPCLDLATSAISRPAETAL
                     YMRSVILNGVDPLSDLTNKTVTGGRLNVRNSMEIIRA"
     assembly_gap    565..589
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<590..1675)
                     /locus_tag="PK198_25540"
     CDS             complement(<590..1675)
                     /locus_tag="PK198_25540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF42190.1"
                     /translation="MQIFTRFLLSLLIWSAIGLAHSAASDVRNDAIPGEIIVRFQPSA
                     EVEDVLEDLGARFGTPLVVKQTLSKRLRIELLGFDPARLPAAALFEALKRRPEVHSLQ
                     WNYPIEFRETPNDPLFDRQWDLARIGLPTVWQETTGGLSARGDTIVIAILDSGFDVNH
                     EDLLENIWYNRFEIPGDGIDNDNNGYIDDVQGWNFAANSATHTRGAHGTSVAGICGAR
                     GNNGIGVTGVNMEVRLMLFTMLSVGNAIAAYDYVVEQRSRYNATNGAQGSFVVVTNAS
                     WGQSRVFCTQQPVWAAMLDELGETGILTAAAVSNENFDVEILGDMPATCPTDFLISVL
                     NTNQEDRKHQGSAFGSTSVDLGAPGHGT"
     gene            complement(1803..>2856)
                     /locus_tag="PK198_25545"
     CDS             complement(1803..>2856)
                     /locus_tag="PK198_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42191.1"
                     /translation="RASMSSKKPKAPDTGNVYDRIFKQVMEDLALTILGRFHNLRITH
                     LQRLPEKMHTTTQREADFLCEVVDENSNKFLLHIEFQSALDRNMLARMQEYHGILARK
                     YKLPIRHFVFYFGDEPGPMPSQLEPEMVFTGFELLDFKTIDYRELLNSDTGKEVILAI
                     LADFKNTPADAIIRLIISKLRQVSHSEQELKSSLNQLTILSRLRKLEAETIQTASAMP
                     ITIDIKQDYLYNKGLEEGKLEGKLEGKLEGEAKGKLEAKMVAIIETDMELLCRMAKEN
                     ISYDLMRRLSDVPEPFLAAFRTAWSADKADVLLALVAQAHEFSSIANIHQHIRDALIG
                     FGIPKEEAMTYLEGVV"
BASE COUNT          645 a          688 c          733 g          692 t
ORIGIN      
        1 ttttattttt ttatcgcccc ttagtgagcc accacaaatc gctgtgtaag gagttgttcg
       61 ccatgcgcga gggtgataaa atatactccg gcgggccaac tatgcacgtc aatttgttgg
      121 gttttctctc caaacctcgg cggtcgcaca tctctgctca gcaccaaacg ccctaaagcg
      181 tcaaaaatac gaatttggta cgcaggttca taatcgggcg cttcgtaagt aatgatcaat
      241 tgctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnncg gcccgaatga tttccataga attgcgcaca ttgaggcggc
      361 cgccggtgac ggttttgttg gtcaggtcgg agagcgggtc cacgccgttg agaatgacgc
      421 tgcgcatata cagcgcggtt tcggcggggc ggctgatggc cgatgtggct aagtctaaac
      481 agggcaggct gtacagcagg gcaatggcgc cgctgacatg cggagtagct gcggaggtgc
      541 ctccgaactc gccgtattcg ttttnnnnnn nnnnnnnnnn nnnnnnnnnt gtgccatgcc
      601 ccggcgcgcc cagatcaacc gaagtgctgc caaatgcaga gccttggtgt ttgcgatctt
      661 cctgattggt attcaacaca ctgattagaa agtcggtggg acaggtagcc ggcatgtctc
      721 ccaatatctc cacatcaaag ttttcattgg aaacggcggc ggcagtgagg atgcctgtct
      781 cacccagttc atccagcatg gctgcccaaa cgggttgctg cgtgcaaaaa acgcgggact
      841 gcccccagga agcattggtg accaccacga aagagccttg cgctccgttg gtcgcgttgt
      901 accggctgcg ttgttctacg acgtaatcat acgcggcgat ggcattgccc acagaaagca
      961 tggtgaacaa catgagccgt acctccatat tgacaccagt gacgccgatg ccgttgttgc
     1021 ctctcgcccc acagataccg gctaccgaag taccgtgagc accacgggta tgcgtagccg
     1081 aattggctgc aaaattccaa ccctgcacat cgtcaatgta gccgttgttg tcgttgtcta
     1141 taccgtcgcc gggaatttca aagcggttgt accaaatgtt ttccagcagg tcttcgtggt
     1201 tcacatcgaa gccgctgtcc aaaatggcga tgacgatggt gtcgccgcgc gcgcttagtc
     1261 cgccggtcgt ttcctgccac acggtgggca gcccgatgcg agccaggtcc cactgcctgt
     1321 cgaagagcgg gtcattgggc gtttctctga actcgatggg gtaattccat tgcagagaat
     1381 gtacctccgg gcggcgcttc aaggcttcaa aaagcgcagc ggcgggcagg cgggcaggat
     1441 cgaagcccag cagttctatg cggaggcgct tgctgagtgt ttgtttgacg accaagggcg
     1501 tgccgaatcg ggcgccgagg tcttcgagta cgtcttccac ctcggcagag ggctgaaagc
     1561 gaacgatgat ttcgccggga atggcgtcat tccttacatc ggaagcggcg gaatgggcaa
     1621 ggccaatggc actccaaatc aggaggctca ataaaaatcg cgtgaagatt tgcatagacg
     1681 ggaccaaatt tgtgtgccaa cagaacggca cagcacctgc tttggttttt tagcttctaa
     1741 acgttaacga aattggcaca cagagaacat actcataatg aaaggggaat gaatttttta
     1801 aattacacca cgccttccag atacgtcata gcttcttctt tcggaatacc gaaaccgatt
     1861 aacgcgtccc ttatatgctg atggatattg gcgatgctgg aaaattcatg tgcctgcgca
     1921 accaaagcca gaagtacatc cgccttgtcg gcagaccagg cagtacggaa agctgccaga
     1981 aacggctccg gcacatcgct gaggcggcgc ataaggtcat aagagatgtt ttccttagcc
     2041 atacggcaaa gtagttccat gtccgtttct ataatggcga ccatcttggc ttcgagtttg
     2101 cctttagcct cgccttcgag tttaccttcg agtttgcctt cgagtttacc ttcttccaaa
     2161 cccttgttgt aaaggtaatc ctgtttgatg tctatggtaa tcggcatagc agatgcggtt
     2221 tgtatggttt cggcctccaa tttacggagt ctggataata ttgtcaactg gttgagacta
     2281 ctttttaatt cctgctcgct gtggcttacc tgtcgaagtt tgctgataat gagccggata
     2341 atggcatcgg cgggagtgtt tttgaagtct gccaaaatag ccaaaatcac ttctttgcct
     2401 gtgtctgaat tcaacaactc cctgtagtca atggttttga aatccaacaa ctcgaagccg
     2461 gtgaaaacca tttctggttc taattgtgag ggcattggcc ccggttcatc gccaaaatag
     2521 aacacaaaat ggcgaatggg cagtttatac ttacgagcca gaattccatg atattcctgc
     2581 attcgagcga gcatgttccg gtcgagggca ctctgaaact ctatgtgaag caaaaatttg
     2641 ttactgtttt catccaccac ttcacagaga aaatcagctt ctcgctgggt ggtggtgtgc
     2701 attttttcag gcagccgctg caaatgtgtg atgcgcaggt tgtgaaatcg ccccaaaata
     2761 gtaagggcca aatcttccat tacctgcttg aaaatgcggt cataaacgtt tcctgtgtcg
     2821 ggagctttgg gctttttaga agacatagag gcgcgg
//
LOCUS       DAORLF010000542         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19109_length_2853_cov_1.941029, whole genome shotgun sequence.
ACCESSION   DAORLF010000542 DAORLF010000000
VERSION     DAORLF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19109_length_2853_cov_1.941029"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK198_25550"
     CDS             complement(<1..189)
                     /locus_tag="PK198_25550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF42192.1"
                     /translation="MDVSVIVPLFNEEESLPELEAWIRRVCLENALSYEIIFVDDGST
                     DGAPQALHLRMVWDAARVA"
     gene            complement(335..>710)
                     /locus_tag="PK198_25555"
     CDS             complement(335..>710)
                     /locus_tag="PK198_25555"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRF42193.1"
                     /translation="YIVAMVKAAMVERDASGGAFPFREALRPAFGSYVIASLVYVAFN
                     YVLYNYVDTNLVEIQREMMIEQSKVLAEKLGSEDMLEQMENISADDLRVGFRNSAVGF
                     LWSLIGGFFLSAIIALFIKREA"
     assembly_gap    711..723
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(850..1419)
                     /locus_tag="PK198_25560"
     CDS             complement(850..1419)
                     /locus_tag="PK198_25560"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRF42194.1"
                     /translation="MSTLDDPIYNAQKPASKWPLALRWGLIGGLAGIALQMLWQVMGW
                     SDYSSSFSAGNLALMPLGWAIGIGATALGIKQYRDDHNGGFISFGGGFGMGMMIALVM
                     ATVGAIFVVTYMGDVMQDAMSTAMQEQFESQGLDDEQIEEATKMAGMFTSTPVMAIFG
                     LIGGLVSGLIYSLIASAIMKRDAPSTYSN"
     gene            complement(1484..>1883)
                     /locus_tag="PK198_25565"
     CDS             complement(1484..>1883)
                     /locus_tag="PK198_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42195.1"
                     /translation="GLEDGTLDFIFSNHLPLDTEAKDREFPYADFGAIGLQTAAAALH
                     THLGLRFSPQQWIKWLALRPREIFGLPAPSVQVGVQAWLTLFQPNAEWTFQREHILSK
                     SHNSPLIGQSFKGRVWATISGPHYRKFDAL"
     assembly_gap    1884..1906
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1907..>2231)
                     /locus_tag="PK198_25570"
     CDS             complement(<1907..>2231)
                     /locus_tag="PK198_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42196.1"
                     /translation="NRPSDAGIAGGGQLHEGTISTRLGLRGIPALAEQLMTERDLHLL
                     EYTGSRLHLWGLSTRGAVEMVRRAKKAGLPVTASVAALNLLFTDEVLGDFNENFKVLP
                     PLRSED"
     assembly_gap    2232..2277
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2278..2770)
                     /locus_tag="PK198_25575"
     CDS             complement(<2278..2770)
                     /locus_tag="PK198_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42197.1"
                     /translation="MNLLLRQATIVDPASPHNGHTTDIHILEGRIEAIGEHLQASGAE
                     VIEGKGAWCSPGWLDFGTQGGDPGLEHREDFNSLSEAALAGGYTTLVCAPNTDPPLHS
                     KSEIAYLMAQNRRLPVQLLPLGAASMHCQGKDITEMLDMRAAGAVAFGDGHTPIANSG
                     LMLR"
BASE COUNT          656 a          727 c          766 g          622 t
ORIGIN      
        1 ggcgacgcgg gcagcgtccc agaccatccg tagatgcaac gcctgcggcg cgccatcggt
       61 gctgccgtcg tccacaaaaa taatttcgta agacagggca ttttcgaggc aaaccctgcg
      121 aatccaggct tccaattcgg gcaaggattc ttcttcgttg aacaaaggca ctattacgga
      181 tacatccatt tttatcattc tttatagctg cgagctacga gtcgcgagct acgagtttgg
      241 ttttccaaaa gaaaaaggat tgattttatg atgagtgaag gggtgactac aggctgtgat
      301 cttgattgag tcaccccttc actaaactac tatgctatgc ctcccttttg ataaacagcg
      361 caatgatggc cgagaggaaa aagccgccga tgaggctcca taaaaagccc acagccgagt
      421 tgcggaatcc cacgcggagg tcgtctgcgc tgatgttttc catttgttcc agcatatctt
      481 cgctgcctaa tttttcagcc agcactttgg attgctcaat catcatttcc cgctggattt
      541 caacgagatt ggtatccaca tagttgtaga gcacatagtt gaacgccaca taaaccaagc
      601 tggcaatgac atacgaaccg aatgccggcc gcagcgcttc ccggaaagga aatgcaccgc
      661 cggaggcatc gcgctccacc atcgcagcct tgaccattgc aacaatatag nnnnnnnnnn
      721 nnngatgagc gaactccagg aaacccaagg gctaagcatc atcttcgggt ctataaagta
      781 gaacaggagc aggtacgcta tggtacccgc tcctgcatac aatccgtgtt ttaaaatgtt
      841 ggcgttcatt tagttggaat acgtggacgg agcgtcgcgt ttcatgatgg cagaagcgat
      901 aagggagtaa atgaggccgg aaaccaagcc gccgatcaat ccgaatattg ccattacggg
      961 agtgctggtg aacataccgg ccattttggt ggcttcttca atttgttcat cgtccaggcc
     1021 ctggctttca aactgttcct gcatggcggt actcatggcg tcctgcatga catcgcccat
     1081 gtaggtcacc acaaaaatag cgccgaccgt agccatgact aaagcaatca tcatgcccat
     1141 gccaaagccg ccgccaaagc tgatgaatcc gccgttgtga tcgtcgcgat actgtttgat
     1201 gcccagcgcc gtagctccaa tacctatcgc ccagcccagc ggcataaggg caagattgcc
     1261 tgcactgaaa ctgctggagt aatcggacca gcccatcacc tgccacaaca tttgcagggc
     1321 gatgccggcc aagccgccta taaggcccca gcgaagggcg agcggccatt tggaagcggg
     1381 cttttgtgcg ttgtaaatag gatcatctag tgtactcatg gtagtgtggt ttttgattca
     1441 atcccgaaga tgaggcgaaa gtattaaacg accaaacgtc tttctacaaa gcgtcgaatt
     1501 ttctgtaatg agggccggaa atcgtcgccc aaacgcgccc tttgaaggat tggccgatga
     1561 gcggcgaatt gtgcgattta gacaaaatat gctcgcgctg gaatgtccac tcggcattgg
     1621 gttggaacag cgtgagccag gcctgaacgc ccacttgaac agaaggagca ggcagcccga
     1681 aaatctcccg cgggcggagg gccaaccatt ttatccattg ttgcgggctg aaacggaggc
     1741 ccagatgcgt gtgcaaagcg gcggcggccg tttgcaaacc gatggccccg aaatcggcgt
     1801 aaggaaattc gcggtctttg gcctcggtgt ctaatggcag gtgattgctg aaaataaaat
     1861 ccagtgtgcc gtcttccaaa cctnnnnnnn nnnnnnnnnn nnnnnngtct tcgctgcgga
     1921 ggggaggcag cactttgaaa ttttcattaa aatcgccgag tacttcgtcg gtaaacagca
     1981 ggttgagcgc cgctaccgag gccgtcacgg gcaggccggc ttttttggcc cgccgcacca
     2041 tttctacagc gcctcgtgtg gaaagtcccc acaaatgcag gcgcgaaccc gtgtattcca
     2101 gcaggtgcag atcgcgctcg gtcatcagtt gttcggccaa agccgggatg ccgcgcaacc
     2161 ccaagcgggt actgatcgtg ccttcgtgca actgaccgcc gccggcaata cctgcatcgg
     2221 aggggcgatt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     2281 tgagcatgag gccgctgttg gcaatgggag tatggccgtc gccgaaagct accgcgccgg
     2341 ctgcccgcat atccagcatt tcggtgatgt ctttgccctg gcagtgcatg gaggcagcgc
     2401 cgaggggcag caactgaacc ggcaggcggc ggttttgcgc cattaaatag gcgatttcgg
     2461 atttggaatg gagtggcgga tcggtgttgg gggcgcatac caatgtggta taacctcctg
     2521 ccagtgctgc ctcactcagg ctgttgaagt cttcgcggtg ctccagtccg gggtcgccgc
     2581 cctgtgttcc gaagtcgagc cagccggggc tgcaccaggc gcctttccct tcaatgactt
     2641 ctgcgcccga tgcctgtaaa tgctcgccga tggcctctat tcgtccttcc aaaatgtgga
     2701 tgtctgttgt gtggccattg tgcggcgagg cggggtcaac gatggtagct tggcgcaaca
     2761 gaaggttcat gcggttggaa aatttgcggc aaaactacga agtaaggggc gataaaaaaa
     2821 taaagtgatc attttcgcca aaggattaga taa
//
LOCUS       DAORLF010000543         2850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19147_length_2850_cov_1.250447, whole genome shotgun sequence.
ACCESSION   DAORLF010000543 DAORLF010000000
VERSION     DAORLF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19147_length_2850_cov_1.250447"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /gene="rpsK"
                     /locus_tag="PK198_25580"
     CDS             <1..131
                     /gene="rpsK"
                     /locus_tag="PK198_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRF42198.1"
                     /translation="GPGSGREAAVRAIDAAGIKVTRIQDLTPMPHNGCRPLKRRRV"
     gene            162..>258
                     /locus_tag="PK198_25585"
     CDS             162..>258
                     /locus_tag="PK198_25585"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRF42199.1"
                     /translation="MARYTGPATKKARTFGEAIFGYDRSFEKRKYP"
     assembly_gap    259..304
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <305..>611
                     /gene="rpsD"
                     /locus_tag="PK198_25590"
     CDS             <305..>611
                     /gene="rpsD"
                     /locus_tag="PK198_25590"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRF42200.1"
                     /translation="KQRSDYALQLMEKQKAKYTYGLLERQFRSTFEKASRKKGVTGDV
                     LLQMLESRLDNTVYRMGFAPTRRAARQLVSHKHIVVNGILTNIPSFHLRPGDVIAVRG
                     "
     assembly_gap    612..674
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            919..1917
                     /locus_tag="PK198_25595"
     CDS             919..1917
                     /locus_tag="PK198_25595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRF42201.1"
                     /translation="MSILNFQKPDKIVMQKANDFEGLFEFKPLEPGFGQTVGNSLRRV
                     LLSSLEGYAISAVRIAGVDHEFATIRGVVEDLVDIVLNLKQVRLKHLLQGDDSSTEKV
                     YLTITGKEQFVAGDIEEHTNVFKVMNPDLVICNMEPFVNLEVELTVSKGRGYVPADDN
                     LPKDAPIGVIPVDAIYTPIRNVAYRVENTRVGQRTDYEKLTIEVKTDGTIHPEDAIKE
                     ASKIMIQHLMLITDEHITFDDPARGEENVVDEHILHMRKLLKTSLEDLDLSVRAYNCL
                     KAAKINTLAELVRFDTHELLKFRNFGKKSLVEIEELLQDKGLTFGMDLSKYKLEEE"
     assembly_gap    2118..2145
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2146..>2498
                     /gene="rplQ"
                     /locus_tag="PK198_25600"
     CDS             2146..>2498
                     /gene="rplQ"
                     /locus_tag="PK198_25600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRF42202.1"
                     /translation="MITHKRITTTLAKAKALRIFIEPLVTKSRDNTTHSRRTVFSYLQ
                     NKEAVQEMFGPIAAKVGDRPGGYVRVLHTGFRKGDAAEMAMIELVDFNEIYSAGKDSA
                     AQKEGGKKRSRRGGKG"
     assembly_gap    2499..2630
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2631..2830)
                     /locus_tag="PK198_25605"
     CDS             complement(<2631..2830)
                     /locus_tag="PK198_25605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42203.1"
                     /translation="MMINFDFCIVVFFGIATTGNAKGSVFSKKTKAAQNLHSERPSSY
                     FKLRENYFCSSLFSSVAAASVAS"
BASE COUNT          719 a          567 c          651 g          644 t
ORIGIN      
        1 aaggccccgg ttccggcaga gaagccgcgg ttcgtgcaat tgatgcagcg ggtattaaag
       61 tgactcgtat tcaagacctt acgccgatgc cgcacaatgg ctgtcgtccg cttaagcgca
      121 gaagagttta attattcaga gcaagcaaag tattatttcc aatggcaaga tatactggtc
      181 cggctacaaa aaaagccaga acgttcggtg aggcaatatt cggttatgac cgctcattcg
      241 agaagcgcaa atatcctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnncaagca aaggtctgac tatgcgctcc aattgatgga gaagcaaaag gccaaatata
      361 cctatggact gctggagcgt cagttccgta gtacctttga aaaggcttct cgcaaaaagg
      421 gcgtaacggg tgacgtattg ctccaaatgc tggaatctcg tttagataat actgtttatc
      481 gcatgggttt tgctcctact cgacgagctg ctcgtcagtt agtttcgcac aaacacattg
      541 tggtcaatgg aattttaacc aacatacctt cttttcacct tcgtccgggg gatgtaattg
      601 ctgttcgcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnagaagc gcgcaaatat ccttggttgc agttcaaccc cgacaaaatg
      721 gagggtgttt ttgtggccta cccggagcgc gaccaaattc cggaaaacat caacgtgcag
      781 ttgatcgtcg aattgtactc taagtaagaa gtacttcgca cagccggtga aagccggctt
      841 tgcgatttgt ttataccggt tgcagcaact ttcgcttatt gttgttttct gatcaaattc
      901 acggatacca cttaacatat gagcatactc aattttcaga aacctgacaa aatcgttatg
      961 cagaaagcaa atgattttga agggcttttt gagtttaagc ccctcgaacc gggtttcggt
     1021 caaaccgtcg gcaactcact gcgcagggtg ttgctttctt ctttggaagg ttacgctatt
     1081 tctgctgttc gtattgcagg agtagatcac gagttcgcca ccatccgtgg agtagtggag
     1141 gatttggttg atattgtgct caatctcaag caagtgcgcc tgaagcatct tcttcaggga
     1201 gatgacagtt caacggaaaa ggtttatctc accatcactg gtaaagagca gttcgtggcc
     1261 ggagatattg aggagcatac caacgtattc aaggtgatga acccggactt ggttatctgc
     1321 aatatggagc catttgtaaa tttagaagtc gaactcacgg tttcaaaagg tcgtggctat
     1381 gtgcctgcgg atgataacct gccgaaagat gcgcccattg gagtgattcc tgtggatgct
     1441 atctatacgc ctatacgcaa tgtggcttat cgcgtggaaa atacgcgcgt aggtcagcgc
     1501 acagactatg aaaaactgac cattgaagta aaaaccgatg gtaccattca cccggaagat
     1561 gccattaaag aggcttccaa aattatgatt cagcacctga tgctgatcac cgatgagcac
     1621 atcacttttg acgatccggc tcgcggcgaa gaaaatgtgg tggatgagca catactgcac
     1681 atgcgcaagt tgttgaagac ttctctggaa gacctcgact tgtctgtgcg ggcatacaac
     1741 tgcctgaaag ccgccaaaat caatacgctg gctgaattgg tgcgatttga tacgcatgaa
     1801 ttgctcaagt tccgcaattt tggtaaaaaa tctctcgtag aaattgaaga attgctccaa
     1861 gacaaaggct tgacctttgg tatggacttg tctaagtaca agttggagga ggagtaacag
     1921 atcaggataa gagtcggggt gacgggctat attggcaacg ttctcaccct atggttaatc
     1981 cagcaataac agagtaagca gacggcgcgc caatctccat tacaagctgc tgaaacgtgt
     2041 tgcgaagtaa aaacttattt aatcatgaga cacgggaaga aggataatca cctcggccgt
     2101 accaaggccc accgttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgat tacgcacaag
     2161 cgcatcacta ccacattggc caaagccaag gcattgcgga tattcatcga gcctttggtg
     2221 acgaagtctc gcgacaacac gactcactct cgccgcacgg tgttcagtta cctccaaaat
     2281 aaggaagctg ttcaggaaat gtttggcccg attgctgcga aagtgggcga ccgcccgggt
     2341 ggctatgttc gtgtattgca cactggtttc cgcaaaggag acgccgcgga aatggcaatg
     2401 atcgaattgg tagatttcaa cgaaatttac tctgcgggta aggattctgc tgcacagaaa
     2461 gaaggaggca agaagcgcag tcgtcgtggc ggtaaaggnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaagctacgg
     2641 atgctgctgc aacagaagaa aacaacgaag agcagaagta attctctcga agtttgaaat
     2701 aagatgaagg ccgctcggaa tgtaagttct gagcggcctt cgttttttta gaaaacactg
     2761 aacctttcgc gttgccggtt gtcgctatgc caaaaaacac gacaatgcag aaatcaaagt
     2821 tgatcatcat ggcgggagga cttatggcgg
//
LOCUS       DAORLF010000544         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19173_length_2845_cov_2.294624, whole genome shotgun sequence.
ACCESSION   DAORLF010000544 DAORLF010000000
VERSION     DAORLF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19173_length_2845_cov_2.294624"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..570)
                     /locus_tag="PK198_25610"
     CDS             complement(160..570)
                     /locus_tag="PK198_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42204.1"
                     /translation="MTHQPAFKSWSGYAFENLALRHSLSIKKAMGIHGVITNEYSWTK
                     KKNAHTSGAQIDFIIDRANNCINLFELKFLDAEYEMTEREAASLRKSIHVFREHTDTK
                     KNIFVTMLTVFGAAKNPHYLGIVTNQLTVKYLFL"
     gene            complement(567..821)
                     /locus_tag="PK198_25615"
     CDS             complement(567..821)
                     /locus_tag="PK198_25615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42205.1"
                     /translation="MASYKSETRQVGRKMVHLAKNKAMTKIAGRQAEVELLQQIADLF
                     GKFSPSIKPKRKGFFASWMSFYTSISDSCLPEKPTTVGCN"
     gene            complement(865..>1267)
                     /locus_tag="PK198_25620"
     CDS             complement(865..>1267)
                     /locus_tag="PK198_25620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRF42206.1"
                     /translation="PAELTTSPVLAACVQQLEEYFHGRRRAFDLPLDWGGAPDFYKAV
                     WKELLAIPYGQTRSYQSIAEKLGDINAVRAVGQANGRNPIAIIVPCHRCIAKNGDLQG
                     YFYGLEVKRRLLELENPMSFARQGDLFGGEG"
     assembly_gap    1268..1296
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1384..>2119)
                     /locus_tag="PK198_25625"
     CDS             complement(1384..>2119)
                     /locus_tag="PK198_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42207.1"
                     /translation="TLGWVKRRQWFAKIYYPVYRFYSLITLANLYRPIYYNLIDNKLG
                     KKIGFFLVPYIMLVLWISSVSIDSHVWFPDDPEKTALYKGFYDDLRKEETLVGNASIP
                     SRFVQNGFIELFIRYFPGDDDKALSLKCPDVKLLKKPGLRSDIVVGFNSGMEEPGKDF
                     PEKSLKCLSSIYEISIADSIFTTPVCRFYNHPNMQEKGIITTLDVQYLPRGEHLIQIK
                     KLKKYRKDGKDSLRLVEFTAFPFWKE"
     assembly_gap    2120..2135
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2136..2726)
                     /locus_tag="PK198_25630"
     CDS             complement(<2136..2726)
                     /locus_tag="PK198_25630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42208.1"
                     /translation="MEKENKHKLSEWLDKIQQDSWQLELVITGFSIFLMLGVLDKLDE
                     LRQSVSMASSGAGQLGFFVSILWVVFAGASMFVLINLILHAVLRGLWISSIGLRSVSG
                     DIDFDSLRLAPKFDAFIRRKTGSFDHYIERLENLCSIVFAFTFLIVFVIMSLGLWAFC
                     LGLTLQLLKKTLPETAAQFTGIFAMLVYLITGLLYMA"
BASE COUNT          675 a          663 c          720 g          742 t
ORIGIN      
        1 tatttctcct caaaaaactg cgctatagtt gtttccgctt gaagaccgcg aagtgcagtt
       61 gcacatcgta tgaaaaccgc ctcggccttt tccccgcagg gcaaactgcc aacgcttagt
      121 agtggggcaa aaatgaacat tttatttttt tatcgcccct taaagaaaca aatacttcac
      181 cgtcagttgg ttggtgacga tgccgaggta gtgtgggttt ttggcggcgc cgaataccgt
      241 cagcatcgtg acgaagatat tctttttggt atctgtatgt tccctgaaaa cgtggatgct
      301 tttgcgcagg gaggcagcct ccctttccgt catttcgtat tcggcatcga ggaatttcag
      361 ttcaaacagg ttgatgcaat tgttggccct atcaatgatg aagtctatct gcgctccgga
      421 ggtatgggcg ttcttttttt tcgtccatga atactcattg gtgatcacgc cgtgtatgcc
      481 catcgctttc ttgatagaga ggctgtgccg gagcgcgagg ttttcaaaag cataacccga
      541 ccaggattta aaggccggtt gatgggtcaa ttgcagccaa ctgttgtggg tttttccggc
      601 agacaggaat cggaaatgga agtgtaaaaa ctcatccatg aggcgaaaaa gcctttcctc
      661 tttggtttta ttgatgggga aaatttgccg aacaaatccg cgatttgttg cagcaactct
      721 acctccgctt gccgccctgc tattttcgtc atagctttat ttttcgccaa atgtaccatt
      781 ttccgcccaa cttggcgagt ttcagattta taactcgcca agttgggtct tttttactcg
      841 catttgacga attatagacc tgtctcagcc ctccccgccg aacaaatccc cttgccgggc
      901 gaagctcatc gggttttcta attccagcag ccggcgtttc acctccaatc catagaaata
      961 gccttgcagg tcgccgtttt tggcaatgca gcgatggcag ggcacgatga tggcaatggg
     1021 attgcggccg ttggcctgcc ccacggcgcg aacggcgttg atgtcgccca atttttcagc
     1081 gatggattgg tagctgcggg tttggccgta gggaatggcg agcagttcct tccacacggc
     1141 tttgtaaaaa tcgggcgcac cgccccagtc caaaggcagg tcgaaagctc ggcggcggcc
     1201 atgaaagtac tcctccaact gttgcacgca ggcagccaat acgggcgatg tcgtcagctc
     1261 ggcggggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg ctgatgccca gttcatcgct
     1321 gccggtgatt tcgatgcagc cgatggggga gtggaaatat gttttttcga gcatagtgta
     1381 aaattattcc ttccaaaacg gaaaagccgt aaactccacc aaacgcaggc tgtctttgcc
     1441 gtccttgcgg tattttttga gttttttgat ttgtatcagg tgctcgccgc gcgggaggta
     1501 ttgcacatca agggtggtga tgattccttt ctcctgcatg ttggggtgat tgtaaaaacg
     1561 acataccggt gttgtgaaaa tgctgtcggc gatgctgatt tcgtagatgg aagacaggca
     1621 tttcagactt ttttccggga aatctttgcc gggctcctcc atgccggaat tgaatccgac
     1681 gacgatgtcg gaacggaggc cgggcttttt gagcaatttt acgtccgggc atttcaggct
     1741 cagcgctttg tcgtcatcgc cggggaagta cctgataaac agttcgataa agccgttttg
     1801 tacaaatcgg gaagggatgg aggcattgcc cacgagtgtt tcttctttgc gcaaatcgtc
     1861 gtaaaagcct ttgtacaggg ctgttttttc cgggtcgtcg ggaaaccaga catgggaatc
     1921 aatgctgacc gaactaatcc acagcaccag catgatataa gggacgagaa agaagccaat
     1981 ttttttacct aatttattgt ctatcaaatt ataataaata ggccgataga gattcgccaa
     2041 ggtgatgagg ctgtaaaatc tgtaaacagg atagtatatt ttagcaaacc attgccggcg
     2101 cttgacccag cctaatgtgn nnnnnnnnnn nnnnntgcca tgtacaacaa gcctgtaatt
     2161 aaatacacca gcatagcgaa aatacctgtg aattgtgcgg ctgtttccgg gagcgttttt
     2221 ttcagcaatt gcagggttag ccccaaacaa aatgcccata aaccaagcga catgataaca
     2281 aaaacaatga gaaacgtgaa ggcaaataca atgctgcaaa ggttttcgag gcgttcgatg
     2341 tagtggtcaa aactgcccgt tttacgccgg atgaaagcat cgaatttggg agcgagtcgt
     2401 agactgtcga agtcaatatc gcccgaaacg gagcgcaggc cgatggagct gatccaaagc
     2461 cctctcagta cggcgtgcaa gatgagatta atgagcacaa acatgctggc gcccgcaaat
     2521 acaacccata gaatggatac gaaaaacccg agctgaccgg cgcccgagct tgccatactg
     2581 acgctttgtc gcagttcgtc caacttgtcc aatacaccca acatgaggaa tatgctgaaa
     2641 ccggtaatga ccagttccaa ctgccaactg tcctgctgga ttttgtccaa ccactcgctg
     2701 agtttgtgtt tgttttcttt ttccatgatg ttttttatcc tttgagcgcc agcccttccc
     2761 tgccggccca ttgccggagg tcttctttgc ggatggcggc ggccatgagt tcgggaaaca
     2821 agtcgggtgt gcaggcaaag gcggg
//
LOCUS       DAORLF010000545         2838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19248_length_2838_cov_1.894718, whole genome shotgun sequence.
ACCESSION   DAORLF010000545 DAORLF010000000
VERSION     DAORLF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19248_length_2838_cov_1.894718"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..322)
                     /locus_tag="PK198_25635"
     CDS             complement(104..322)
                     /locus_tag="PK198_25635"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HRF42209.1"
                     /translation="MAGAIHGCDFLMSRDQFEYWQHTQLTVNVVSGRGASFSLEIPLG
                     LRFIIESRLFTEEEADDLRPLRSGRDVL"
     gene            complement(483..>669)
                     /locus_tag="PK198_25640"
     CDS             complement(483..>669)
                     /locus_tag="PK198_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF42210.1"
                     /translation="SRAVKAGRVWVNCYHAYPAHAPFGGYKKSGIGRETHKMMLSHYR
                     QTKNMLISYDKKALGFF"
     assembly_gap    670..814
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<815..>1073)
                     /locus_tag="PK198_25645"
     CDS             complement(<815..>1073)
                     /locus_tag="PK198_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF42211.1"
                     /translation="VQESIYERFMERVVERTKAIKLGHPLDPATMMGAQASNDQYEKI
                     LRYIEIGKEEGCEVLAGGGAAYNEGLEGGYYIKPTILKGNNK"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1084..2004)
                     /locus_tag="PK198_25650"
                     /pseudo
     CDS             complement(1084..2004)
                     /locus_tag="PK198_25650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            2314..>2838
                     /locus_tag="PK198_25655"
     CDS             2314..>2838
                     /locus_tag="PK198_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF42212.1"
                     /translation="MELAKSFYNGRKLQTLVENQTSYTLENAAMHVFETHQQAHEVLL
                     QFEQPVLASMLVGKKVMHLRDHQAFDFLPGESLILPSGELMCIDFPEAAMDTPTRCLA
                     MAVEEKKILDIIALMNETMARAEGREWRFMDYNFHFTNDIGVHHILQRLIFLFTENHA
                     CKDLFVEMTLRELIV"
BASE COUNT          584 a          686 c          763 g          650 t
ORIGIN      
        1 gaggtcgaaa atccgggatg actcatattt cagcataaca tattgatagc cattatttag
       61 tagtggggca aaaatgacca ctttattttt ttatcgcccc tttctacaaa acatcgcggc
      121 ctgagcgcag gggcctcaga tcatcggctt cttcctcggt gaagagtctg gattcgatga
      181 tgaagcgcag ccccagcggg atttccagcg aaaaactcgc cccgcgaccg ctcaccacgt
      241 tcacggtgag ttgtgtgtgt tgccagtatt caaactgatc cctcgacatg aggaaatcgc
      301 agccgtgtat ggcgccagcc acacgtcgtt gtcgtctaac agcagttcgc ctttgggaaa
      361 acacatgggc gaggagccgt cgcaacagcc gccgctttgg tgaaacataa gcgggccgtg
      421 ccgttcgcgc agttggtcta tcacggcttt ggcagcgtcg gtagccaata ctcgctccgt
      481 catcagaaga agccgagggc cttcttgtcg taagagatga gcatgttttt ggtctggcgg
      541 tagtgcgaga gcatcatttt gtgcgtttcg cggccaatgc ccgatttttt gtacccgcca
      601 aacggagcgt gcgccgggta ggcatggtag cagttcaccc atacgcggcc cgctttgacg
      661 gccctcgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgtt gttgcccttc aggatggtcg
      841 gcttgatgta ataaccgcct tcgaggccct cgttgtaggc agccccgccg ccggccagca
      901 cttcgcagcc ttcttctttg ccgatctcga tgtagcgcag aattttttcg tactggtcgt
      961 tggaagcctg cgcgcccatc atggtagcgg gatcgagcgg gtgccccagt ttgatggctt
     1021 tggtgcgctc taccacgcgc tccatgaagc gttcgtaaat gctttcctgc accnnnnnnn
     1081 nnngaatgcg cgaggggcag gtacacacct cgccctgatt gagggcaaac ataaccgcgc
     1141 cttcgaggca tttgtcgaag aaatcgtcat cggcgtccat gacgctttca aagaaaatgt
     1201 tgggcgattt gccgccgagt tccagcgtga cgggcacgat gtttttggag gcatactgca
     1261 tgatgagctg gccggtagtg gtttcgccgg tgaaggccac tttattgaca cgcggactgg
     1321 aagcgagcgg tttgccggct tcgatgccga aaccattgac gacgttgagc acgccgggcg
     1381 gcaggatgcc ttcgatgagt tccatgagga tcatgattcc gaccggcgtt tgttcggcgg
     1441 gcttcagcac gatgcaattg cctgcagcca gtgcgggcgc tattttccag gtagccatca
     1501 gcagcgggaa gttccagggg atgatttgcg ccactacgcc cagcggttcc agaatgttca
     1561 tggaaacggt attggcatcc aactcactga cggagccttc ttcagcgcgg atgacgccgg
     1621 cgaaatagcg gaagtggtca atggccagcg ggaggtcggc ggcgcgggtt tcgcggaggg
     1681 ccttgccgtt gtcccaggtt tcggcgcggg ccagcgcttc gaggttggcc tccatcacat
     1741 cggcaatttt gaggaggagc gggtggtagc cgaggaggca ttccaggaag gcgcggcagc
     1801 ccaggcggca tcaagggcca gttcgatgtc ttcttcggta gagcgggcca ctttggtaaa
     1861 gatttggccg tccacaggag agaggttttc aaagtactct ccgcgcacgg ggggcgtcca
     1921 tttgcctccg atgaagttgt cgtactggtc tttgaatttt ggtttttcca cggcgttggc
     1981 ggtggtggtg agaatgtcgc tcatgttaat tgtttattta gttaaggtga aaaaatcatg
     2041 ttgtagctgc gatcctccaa tgtattccga tgtgctcgca ctttaaagga tatgttgaac
     2101 agcaactttt ctaaaattat ccacgtcgtt tcgttgatga tgcaaaattg gatacggaag
     2161 atctgtgcga gtttgaacga ttccggcaaa aaggtgtaca atactcgcaa gcgcatcggc
     2221 gtcgggcatt ttttgcggaa tatttggacg gaagtcttat ttttggggca taatcgttgc
     2281 attttagtcc taaccattga aatattaaac ggcatggaat tggccaagtc cttctacaac
     2341 ggcagaaaac tgcaaacttt ggtggaaaac caaacctcct acacgctgga gaacgccgcc
     2401 atgcacgttt ttgaaaccca ccaacaagca cacgaggtat tgctccagtt cgagcagccg
     2461 gtattggcca gtatgctggt gggcaaaaag gtcatgcacc tgcgcgacca tcaggctttc
     2521 gacttcctgc ccggcgaatc gctcattctg ccttccggcg aactgatgtg cattgatttt
     2581 cccgaagcgg cgatggacac gcccacccgc tgcctcgcta tggcggtaga ggagaaaaag
     2641 attttagaca tcatagcgct gatgaacgaa accatggcgc gggccgaagg gcgcgaatgg
     2701 cgcttcatgg attacaactt ccactttacc aacgacatag gcgtacacca catcctgcaa
     2761 agactcattt ttttgttcac cgaaaaccac gcctgcaaag acctctttgt agaaatgacg
     2821 ctccgggaac tgatcgtg
//
LOCUS       DAORLF010000546         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19319_length_2831_cov_1.878242, whole genome shotgun sequence.
ACCESSION   DAORLF010000546 DAORLF010000000
VERSION     DAORLF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19319_length_2831_cov_1.878242"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK198_25660"
     CDS             complement(<1..804)
                     /locus_tag="PK198_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42213.1"
                     /translation="MAGGHIRVNRIGIYDIEGIPCLINFGTFTNDANIAVGNDVLLYG
                     QDGIFNAGSFSNSSTGIISIEKPWGNGIWNFSGNFQNAGKITIKNIFYFEDGGFSTGI
                     LSTAAFTNQAGAEIHIDQVASGIISTRAFTNAGLIRMGENGPLAGSGIANIQGANAIF
                     NNNAGGDISIKQTSADGIQNEANSTFNNNACATLTVFDNLNNTGTFTNTGLFTVNTTQ
                     THTNSALTNNGLIAYPQGNLIPNVTNNEIIIAPTTANACDVISPAFGLGS"
     assembly_gap    933..943
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<944..2508)
                     /locus_tag="PK198_25665"
     CDS             complement(<944..2508)
                     /locus_tag="PK198_25665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42214.1"
                     /translation="MKKHIHPCSEKTPWFRQTGPSTPYAIALALALALGHVAAFAQIS
                     IPNAGFTYTQDFNTLAVSGASTAVPAGWAFFEQSFFGCSGCANTTYRAGDGSLFFFYD
                     TYSFGTGTNSDRTFGSQGSVTGDSLRTTIGVCFTNNTGATITSLTASFTGETWAVNAP
                     NRIDGLQFSYNQNTTAINGAGTWTLFSGLDYYNPGSSTLDRGSVQHSALITATISGLN
                     IAPGNTFCFRWLDIDAIPSVPNQNLEDALGIDDFSLSNIVVCDPPAISLPAVTQPTCA
                     TPTGTIVVNATGIGVLEYSINNGSTWQTANTYSGQAPGNYNIKVRLQANPSCEATYSS
                     NPVMLNPPFTASTTTDTWTGCVSTDWATPGNWADGSVPTAADDVTIPNVANDPIILGG
                     TAAVARSMLVQAGAVLTLNTMGTLTINNSAVHGLDNYGTVDNSGSIILGNTTAIGAIG
                     IVHRNGGTFNNKPDGIIQIDRTLGAQAFYSNGLVNNEGAVEIGSNAAVSSEGIRLEGN
                     TFNNKPAGSIKID"
BASE COUNT          658 a          669 c          762 g          731 t
ORIGIN      
        1 actgcccaga ccgaaagccg gactgatgac atcgcaggca ttggccgtgg tgggtgcaat
       61 gatgatctcg ttgttcgtta cattggggat gagattgccc tgcgggtagg cgatgaggcc
      121 gttgttggtg agggccgaat tggtgtgggt ctgggtcgta ttgacggtaa acaggcctgt
      181 atttgtgaac gtacccgtat tgttgaggtt gtcaaagacc gtgagcgtgg cgcaggcgtt
      241 gttgttgaag gtcgaatttg cctcattttg tatgccatct gcggacgttt gtttgatgct
      301 gatgtcgcct ccagcattgt tgttaaatat ggcattagct ccttgtatgt tggcaatacc
      361 acttcccgct aagggtccat tttcccccat ccgaatcaag ccggcattgg taaatgctct
      421 ggtgctgata atcccactgg ccacctggtc tatgtgtatt tctgcccctg cctgattggt
      481 aaaagcggca gtactgagaa taccggtgct aaagcccccg tcctcaaaat agaagatgtt
      541 cttaatcgtg atttttccgg cattttggaa attaccggag aagttccaga taccattgcc
      601 ccagggtttc tcaatagaaa tgatacctgt ggaactgttg gaaaaactac cggcattgaa
      661 aataccgtcc tgcccgtaaa gaagtacgtc attgccgacg gcaatattcg catcgttggt
      721 aaatgttccg aaatttataa gacaggggat gccttctata tcataaatac ctatccgatt
      781 gacgcggata tgcccgccgg ccatgttatt aaaggtacaa ccacttccaa ttctaatgcc
      841 attacctctt acatccgatt gattaccaat ctcaataagc gcctcattgt tcagtattgt
      901 accagtgttg accaatcgaa tggcgtggct tcnnnnnnnn nnntggtcaa ttttaataga
      961 gccggccggt ttgttgttga aggtatttcc ttccagtctg ataccttctg agctgacagc
     1021 agcattgctt ccaatttcga cggcgccttc attgtttact aagccattgg aataaaacgc
     1081 ttgtgcacca agtgtccggt caatttgaat aataccatct ggtttgttgt tgaaggtacc
     1141 gccattgcgg tgcacaatgc ctatagcgcc aatagcagtt gtatttccca agatgatgga
     1201 tccgctgttg tccactgtgc cataattatc caagccatga acagccgagt tattgatcgt
     1261 caaagttccc attgtattaa gggtgagaac agccccagcc tgcaccagca tcgacctggc
     1321 cacggccgcc gtgccgccca agatgatcgg gtcattggcc acattgggta tcgtcacatc
     1381 gtcagcagcc gtgggcacac tgccatccgc ccagttgccg ggcgtggccc agtcggtcga
     1441 gacgcagccc gtccacgtat ccgtagtggt ggaggcggta aacggcgggt tcaacatcac
     1501 ggggttgctg ctataggtgg cttcacagct tgggttggcc tgcaagcgca ctttgatgtt
     1561 gtaattgccc ggggcttgtc cgctgtaagt attggcggtt tgccaggtgc tgccgttgtt
     1621 gatgctgtat tccagtacgc cgatgccggt ggcgttcacc acgatggtgc ccgtaggggt
     1681 ggcgcaagtg ggttgggtga cggcggggag agaaattgcc ggcgggtcgc aaacaacaat
     1741 attgctcaaa gaaaaatcat ctataccgag ggcgtcttcc aggttttggt ttggaacgct
     1801 tgggatcgcg tcaatatcca accaccggaa gcaaaaggta ttgccgggtg caatattcaa
     1861 tcccgaaatg gtagctgtaa tgagagccga atgctggact gaaccgcgat caagcgtaga
     1921 ggagcctggg ttgtaataat ccaatccgga aaagagcgtc caggtgccgg cgccgttgat
     1981 ggccgtggta ttctggttgt aggaaaattg gagtccgtct attcggttag gcgcattcac
     2041 ggcccaggtt tctcctgtaa aggatgcggt cagcgaagta atggtggcgc cggtattgtt
     2101 ggtaaaacag acgccgatgg tcgttcgtag cgaatcgcca gttacgcttc cctgactgcc
     2161 gaatgttctg tccgaattcg tgccggttcc gaaactgtaa gtgtcataga aaaaaaagag
     2221 tgacccatcg ccggcacgat aagtcgtgtt ggcgcaacca ctacacccaa aaaaactttg
     2281 ttcaaaaaaa gcccagccgg caggaacggc ggtgctggct ccggaaacag ccagcgtgtt
     2341 aaaatcctgc gtgtaggtaa agcccgcatt cggaatggaa atttgcgcga aagcggccac
     2401 gtggcccaac gcaagggcaa gcgccagcgc aatggcataa ggagtgcttg ggccggtttg
     2461 tctgaaccac ggcgtcttct ctgagcatgg atggatgtgt ttcttcatgt tttgattttt
     2521 tttcttaatt tgaactgcct gccagaggtt acctctttgc gggggtaaac ctcagtaaaa
     2581 accagccgat ctgcgattgc tactttccga atgacgggtt tgagtttcca agtgacgggt
     2641 ttgggtttcc aaatgaactg acaataatca ccccgcccgt cggccaattg tcatttgctg
     2701 tttgtaacca agcctctttc tttgccggga atctaaccat aataaaacat gagcgagcat
     2761 aaattcagaa ccatgggcga acaggccggg cgacgttcct gggccgaacc ctggaaaatc
     2821 aaaatggtgg a
//
LOCUS       DAORLF010000547         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19423_length_2820_cov_1.150814, whole genome shotgun sequence.
ACCESSION   DAORLF010000547 DAORLF010000000
VERSION     DAORLF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19423_length_2820_cov_1.150814"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK198_25670"
     CDS             complement(<1..598)
                     /locus_tag="PK198_25670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42215.1"
                     /translation="MRIAVIALFTMLLIDKVGFTQKTLPPLPPPPPKIGAYDTTILQG
                     YIAEKIALKEREEKLVAALLAEIRNTAHPELKERLILLLGDFDNEEATRFLVNNLTYR
                     SRYVEEGGVEASFMHLPCLMALISKKSLHALPFIEEYLRECRTNREITLLYMVLSNYI
                     VKLRLDIRWIKEMTPSVPENTCKEKNVERLISHLKQNRF"
     assembly_gap    862..885
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <886..>2041
                     /locus_tag="PK198_25675"
     CDS             <886..>2041
                     /locus_tag="PK198_25675"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF42216.1"
                     /translation="DVQGNNTDVPGYQTTGTGLTYSDLQTSGTAASGSRSYLTAGRMI
                     DVSAGGPFASFLSNDGIGLAGRDLYVAALLSKDQNNNEEVWLGLHRSNIPWYFHQNRV
                     AMGYFGSHSDNGGTRYWTLRVEDNYYQTTVPVSIGTTSLLVMKIAFGSNNQHTISLYV
                     NPADLGDTEPAPTLVQAVQTPLSLTAMAFYAGSSAGNGRLDEIRMAASYRCATPDADV
                     FVNQLPVASFTSSAADGNAPFQVQFNSSASSDADGTISSRQWQFGDGGSSAQNPSYTF
                     QNPGYYQVTLTVTDNCGDQSGSAQWIVVRDADGQFPCGSAVALSSPASPGQNNGGISC
                     NSSYGSQFQLTGSGGLNISQSSGQFNNLSAGSYTLTVSGANGCQDVFSLQV"
     assembly_gap    2042..2072
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2073..>2358
                     /locus_tag="PK198_25680"
     CDS             <2073..>2358
                     /locus_tag="PK198_25680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42217.1"
                     /translation="GWIPNLCDLQLGVNLDGTPYWNKIRPFKNLKLESGEFFSADASG
                     GPWNSGVMSQIPVDANGYPTQLPYTTSRGASIVRCVISADGHLPPGQYVLL"
     assembly_gap    2359..2392
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2393..>2820
                     /locus_tag="PK198_25685"
     CDS             <2393..>2820
                     /locus_tag="PK198_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42218.1"
                     /translation="GSVTVSSNTPGRIAFQLFGEGNQWFHITSSQSGNHIRNIRILRA
                     AHETDDLSDPFYDVFLEKVNRFTTLRFMDWGHTNGSELSEWSQRTDPDHHTMGEGDGV
                     AYEWMIALANRLQKDVWICVPHQASENYITQLAPLFRNQL"
BASE COUNT          640 a          789 c          666 g          636 t
ORIGIN      
        1 tgaacctatt ttgttttaaa tgactgatca atctttctac atttttctct ttacaggtat
       61 tttcaggtac acttggtgtc atttctttaa tccacctaat atccaaccta agtttgacga
      121 tgtaattaga caacaccata taaagtaagg ttatttctct gtttgtccga cactctcgaa
      181 ggtactcctc aatgaaaggt aaagcatgta aggatttttt tgatataagt gccattaaac
      241 agggtaaatg catgaaagaa gcctcaacgc ctccttcttc tacatatctg ctccggtagg
      301 tgaggttatt taccaaaaat ctggttgcct cctcattatc gaaatccccc agtaatagaa
      361 tcagcctttc tttcagttca ggatgcgcgg tatttctgat ttccgccaac agagcagcaa
      421 ccaatttttc ctcccgctct ttcaatgcga ttttctcagc gatataacct tgtagaatcg
      481 ttgtgtcgta agctcctatc ttaggcggcg gcggtggtaa aggcggaagc gttttttgag
      541 taaatcctac tttgtcaatc agcaacatgg taaataaagc aatcacggcg attctcattt
      601 gatcattttt taaacaacaa gatgcgcgat gcgtgtaatg agttgtaacc acgatttcca
      661 aaaaaatgaa aaattacccc gttctttgcc ccatcaatca accaatctcc gcttctcaaa
      721 atcatgttca cacgcatcct caccctgctc tccgtcctat tttacgctgc tgtcgcgcag
      781 gcacagggcg gctgccccgg cgcctgtttc atcatggcct atgaaggatt tcaatacccg
      841 gcgaatgcgc ccctgcacgc gnnnnnnnnn nnnnnnnnnn nnnnngggat gtgcaaggca
      901 acaacaccga tgtaccgggt taccagacaa ccggtaccgg actgacttac agtgacttgc
      961 aaacatccgg tacggcagca tcgggttcgc gatcctacct caccgccggg cgcatgatag
     1021 acgtttctgc cggagggccg ttcgcgagtt ttttgtccaa cgacggcatc ggcttagccg
     1081 ggcgcgatct gtatgtggcg gccttgctga gcaaagacca aaacaacaac gaagaagtgt
     1141 ggctgggcct gcaccgcagc aatatcccct ggtatttcca tcaaaaccgg gtggcgatgg
     1201 gctatttcgg ctcgcactcc gacaacggag gtacccgcta ctggaccttg cgcgtggagg
     1261 acaattacta ccagacgacc gtacccgtga gcatcggcac gacgagcctg ttggtgatga
     1321 aaattgcctt cggctccaat aaccagcaca ccatcagcct gtatgtcaac cccgccgact
     1381 tgggcgatac cgagccggcg ccgactttgg tgcaggcggt acaaacgccg ctctctctca
     1441 cagcaatggc cttttatgcg ggcagcagtg ccggaaacgg gcgcttggac gaaatccgca
     1501 tggcagcctc ttaccgctgc gccactcccg atgccgatgt gtttgtcaat caactacctg
     1561 tggcctcttt caccagcagc gccgccgacg gcaacgcgcc ttttcaggtg caattcaaca
     1621 gcagcgcctc ttcggatgcc gacgggacaa tcagttcccg gcaatggcaa ttcggcgacg
     1681 gcggcagtag cgcccaaaat ccgagctata ccttccaaaa tccgggctac tatcaggtga
     1741 cgctcaccgt gaccgacaac tgtggcgacc agtccggcag cgcgcaatgg atcgtggtgc
     1801 gggatgccga cgggcaattc ccctgcggtt cggcggtggc cttgagcagt ccggcatcac
     1861 cgggacaaaa caacggcggc atcagttgca attcttccta cggcagccag tttcaactca
     1921 caggcagcgg cggcctcaac atcagtcaaa gcagcggcca gttcaacaac ctctctgccg
     1981 gcagctacac cctcaccgtg agcggcgcca acggctgtca ggatgtgttc agcctgcagg
     2041 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggctgga tacccaacct ctgcgacctg
     2101 caattgggcg tgaatttaga cggtacgcca tattggaaca aaatccggcc gtttaaaaat
     2161 ctcaagttgg aaagcggaga gtttttctcc gcggatgctt ccggcggccc ctggaattca
     2221 ggcgtgatgt cgcaaattcc ggtggatgcc aacggctatc ctactcaact cccttacact
     2281 acctcgcgcg gcgccagcat cgtgcgttgc gtcatttctg ccgatgggca tttgccgccc
     2341 ggtcagtacg ttttgctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaggctcg
     2401 gtcaccgttt cgtccaacac gccgggcaga atcgctttcc aactgttcgg tgagggcaat
     2461 cagtggtttc acatcacctc ctcgcagtcg ggcaatcaca ttcgcaacat cagaatcctg
     2521 agggccgcgc atgaaaccga cgacctcagc gacccgttct acgacgtatt tttggaaaaa
     2581 gtgaaccgct tcaccacctt gcgtttcatg gactgggggc ataccaacgg cagcgaactc
     2641 agcgagtggt cgcagcgaac cgaccccgac catcatacca tgggcgaagg cgacggcgta
     2701 gcgtatgaat ggatgatcgc cttggccaac cgcctccaaa aagatgtctg gatttgcgta
     2761 ccgcaccagg cctcggaaaa ctacatcacc caacttgcgc cgctgttccg caatcaactg
//
LOCUS       DAORLF010000548         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19493_length_2812_cov_2.224157, whole genome shotgun sequence.
ACCESSION   DAORLF010000548 DAORLF010000000
VERSION     DAORLF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19493_length_2812_cov_2.224157"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1474
                     /locus_tag="PK198_25690"
     CDS             <1..>1474
                     /locus_tag="PK198_25690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRF42219.1"
                     /translation="GLIAADNTPWWHFYDFAVQQAGGVPVPIHAVATDAEVAYILRDT
                     ELRFCFASNEYLAARVQENAPETLEKVWLFEGPEALPLQPVGEEEQRQLEAVRASIGE
                     ADLATIIYTSGSTGEPKGVMLSHQNMVSNIKGVIALVPVDFTHRVFSFLPLSHVFERM
                     VTWSYMAAGATIYYAEQSGTLMRDIREARPHYFTAVPRVLEKFYSGIQAAVAKRPSWV
                     RRISRWAIGIGHAYKKGKKFPPAYWVQLFLADLLVFRHWRNALGGHVQGIMTGAAALR
                     PQLGRLFSAAGIQIREGYGLTETSPVISFNRFDPGGVRFGTVGIPIPGVEVKIEQPDE
                     NGEGEICVRGPGVMMGYYRRPKETAEVLNEEGWFRTGDVGKFVHKRFLQITDRQKDLF
                     KVSGGRYVAPQLVEKAMLESPYIENCMVFGADRPYVTALILPDFELLKNWCETNGVHW
                     TAPQFMAIHPKVIKWMEAEVERCNETLAHYQRVKSFVLVHE"
     assembly_gap    1475..1488
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1613..1735)
                     /locus_tag="PK198_25695"
     CDS             complement(1613..1735)
                     /locus_tag="PK198_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42220.1"
                     /translation="MSEIDNVGKKGYCMLYFFLFLILFIFLVVWIYNRNSELLQ"
     gene            1837..>2812
                     /locus_tag="PK198_25700"
     CDS             1837..>2812
                     /locus_tag="PK198_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF42221.1"
                     /translation="MNEALQILRPPAEAAYAAELNALAAADTHPRPAGWRLSPWAVVT
                     YIMGGALPDGQEIEPKYFGDRRLIEIAVATLATDRALLLLGIPGTAKTWVAEHLAAAV
                     SGDSTLLVQGSAGMSEDLLRYGWNYARLLSDGPSEAALVPSPVMHAMQHGKIARVEEL
                     SRIPSDVQDTLITILSEKTLPVPELNTAVQARQGFNVIATANDRDKGVNELSSALKRR
                     FNTVVMPLPASLEEEVAIVQNRVEKTGRALQLPVEAAPLQEIRRIVTIFRELRSGKTE
                     DGRTRVKSPGSTLSAAEAISVVTNGQALAVHFGDGVIKAADVAAGLTGA"
BASE COUNT          637 a          765 c          809 g          587 t
ORIGIN      
        1 ggactgattg ctgccgacaa tacgccctgg tggcactttt atgattttgc ggtgcagcag
       61 gccggcggcg tgccggtgcc catacacgcg gtagctaccg atgccgaagt agcctatatt
      121 ttgcgcgata ccgaactgcg tttttgtttt gcatccaacg agtacctagc ggcccgtgtc
      181 caggaaaacg cccctgaaac gctggaaaag gtatggctgt ttgaaggccc cgaagcattg
      241 ccgcttcagc cggtcggaga ggaagagcag aggcaattgg aggcggttcg ggcaagcatc
      301 ggcgaggcgg atttggccac cattatctat acatccggtt ctaccggcga gccaaaaggc
      361 gtcatgttgt cgcaccaaaa tatggtgagc aacatcaaag gcgtcattgc attggtgccg
      421 gtggatttta cccatcgggt gttcagtttc cttcctttga gtcatgtttt cgagcgcatg
      481 gtcacatggt cttacatggc agccggcgct actatttatt acgccgagca gtcggggacg
      541 ctgatgcgcg acattcgcga ggcccggcca cactatttta cggcagtgcc cagggtattg
      601 gaaaaatttt actccggcat tcaggcggcg gtagccaaac gcccttcctg ggtacggcgc
      661 atttcccgct gggccatcgg catcggccat gcgtacaaaa aaggcaaaaa attcccgccg
      721 gcctattggg ttcaactgtt ccttgccgat ctgctggtat tccggcattg gcgcaatgcg
      781 ctcggcggcc atgtgcaggg cattatgacg ggggcggcgg cgctcagacc acaattgggg
      841 cgcctgtttt cggcagccgg cattcagata cgcgaaggct atggcctgac ggaaacttcg
      901 ccagttatct cgttcaatcg gttcgatccg ggcggcgtgc gtttcggcac ggtgggcatt
      961 ccgattccgg gtgtggaggt aaaaattgag cagcccgacg aaaacggcga aggcgaaata
     1021 tgcgtgcgcg gccccggcgt gatgatgggc tactaccgac ggccaaagga aactgccgag
     1081 gtgctgaatg aagagggctg gttcagaacc ggcgacgtgg ggaagttcgt tcacaagcgt
     1141 tttttacaaa tcaccgaccg ccagaaagac ttgttcaaag taagcggcgg gcgatatgtg
     1201 gcgccacagt tggtagaaaa ggccatgctg gagtctccat acatcgaaaa ttgtatggtt
     1261 ttcggcgctg atcgtccgta tgtgaccgcg ctcattctgc ccgattttga actattgaaa
     1321 aactggtgcg agaccaacgg cgtacattgg acggccccgc agttcatggc cattcacccc
     1381 aaagtgataa aatggatgga agcagaagtg gagcgctgta atgagacgct ggcgcattac
     1441 cagcgggtaa aaagttttgt cttggtacac gaacnnnnnn nnnnnnnncc atctcacgcc
     1501 cacgatgaaa ctgcgcagaa gtgtgatact gcggcaatac gaaaaagacc tgacggcgct
     1561 ttacaaatga ggagcgcgtt cctgaaagga gccacggaac gcgcagcgga tattattgca
     1621 acaattcgga gttcctgttg taaatccaaa cgaccaaaaa gatgaagagg atgaggaaca
     1681 ggaaaaaata aagcatgcaa tatccttttt ttcctacgtt gtctatttcg gacatggctt
     1741 ctcgctgttt gttattatac ttgttgttga tggccgcaaa gatgaaaatt atttttaaaa
     1801 gttttggaac tttcgccctt caatcttcca accgacatga acgaagcatt acaaatactg
     1861 agacctccgg cagaagccgc ttatgctgcc gaactgaacg ccttagccgc cgccgatacg
     1921 catccccgac cggcagggtg gcgcctttcg ccctgggcgg tagtcaccta tattatgggg
     1981 ggcgctttgc ccgacgggca ggaaattgaa ccgaaatact tcggcgaccg ccgcctcatc
     2041 gaaatcgccg ttgccacctt ggccacagat cgggcattgc tcttgctggg cattcccggc
     2101 accgccaaaa cctgggtagc cgaacatctg gcggctgccg tgagcggcga ttccaccctc
     2161 ctggtacaag gcagcgccgg catgagtgaa gacctgctgc gctatggctg gaactacgcc
     2221 cgcctactgt ccgacggccc ctccgaagcc gccttagtgc ccagccccgt gatgcacgct
     2281 atgcagcacg gcaaaatagc ccgcgtggag gaactcagcc gcatcccctc ggatgtgcag
     2341 gacacgctca tcaccattct ttctgaaaaa acgctgccgg taccggagct aaacaccgcc
     2401 gtgcaggcgc gtcaggggtt caatgtcatt gccaccgcca acgaccgcga caaaggcgtg
     2461 aacgaactgt cgagcgcgct caaacgccgc ttcaatacag tggtgatgcc gctgcccgct
     2521 tccttggaag aggaggtggc cattgtgcaa aatcgggtgg aaaaaaccgg tcgggcgctc
     2581 caactgccgg tagaagccgc gcctttgcag gaaattcgtc gcatagtcac cattttccgc
     2641 gagttgcgca gcggcaaaac cgaggacgga cgcacccgcg tcaagtcgcc gggcagcacc
     2701 ctgagcgctg cggaggccat ttcggtggtc accaatgggc aggcattggc cgttcacttc
     2761 ggcgacggcg tcatcaaggc ggccgacgtg gctgcgggcc tcaccggcgc ca
//
LOCUS       DAORLF010000549         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19653_length_2795_cov_1.635036, whole genome shotgun sequence.
ACCESSION   DAORLF010000549 DAORLF010000000
VERSION     DAORLF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19653_length_2795_cov_1.635036"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>547
                     /locus_tag="PK198_25705"
     CDS             <1..>547
                     /locus_tag="PK198_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42222.1"
                     /translation="GESASLSVTTASSAITWSNGATGAELTVTEGGWYSAVVGTPGCQ
                     TITPAIEFTVNPPVFPTISVQGDTIFCPGGSATLTASPSSSYTWSNGAATASIVVSAA
                     GSYTVDVQGLCGTFTSQPVTISLLDAPLPAAENDTITGAGQAVLTATGEMPRWYDVPS
                     GGTPVATGNTFTIPMLTATDTF"
     assembly_gap    548..641
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <642..>1017
                     /locus_tag="PK198_25710"
     CDS             <642..>1017
                     /locus_tag="PK198_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42223.1"
                     /translation="QIIFDAHRSFRLATVKVNTDMAGVRIIELRSGNTVLEARTIDIP
                     AGESVITLDMNIPQGNDLILTTNTAQNQSVFGFASPRLRRTSSGVMYPYVTPNVVTLK
                     TSNFGNDVYYYFYNWEIKQPDTE"
     assembly_gap    1018..1069
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1070..1346
                     /locus_tag="PK198_25715"
     CDS             <1070..1346
                     /locus_tag="PK198_25715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42224.1"
                     /translation="NAAQEAWLGDTRLTLSPNPTSEATVLNLSHPLHETANLTLTDMN
                     GRILSRAVLPAGQTQYRWDVQALTPGVYHLRMATREGTAVRALVVTR"
     gene            1357..>1900
                     /locus_tag="PK198_25720"
     CDS             1357..>1900
                     /locus_tag="PK198_25720"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRF42225.1"
                     /translation="MAAYFHGRQFDTFEFHALEKGEYEACRFTNGSGHKADLSEFKFI
                     DCEFVVCDLSLAKLNRTAFREVQFRECKLSGLHFESCHDFGLAFGFELCRIDFCTFIG
                     MKLKGTRFVRSQLLESDFTDCDLTGALFDQTDLSRVVFDNTILEKADFRTAYGYQIDP
                     EQNRMRQAKFSASGLAGLLGK"
     assembly_gap    1901..1923
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2000..>2341
                     /locus_tag="PK198_25725"
     CDS             2000..>2341
                     /locus_tag="PK198_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF42226.1"
                     /translation="MKSPFRPSTSQNAGWNLAKTALQTSVFWFIFLYVFPQLILKMER
                     ELQWPSFSFPEWIAWLLLLVFSVLGVYSGYAMSRFGQGTPLPTDCANELVVQGPYKIV
                     RNPMAVAGIGQG"
     assembly_gap    2342..2454
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2533..>2795)
                     /locus_tag="PK198_25730"
     CDS             complement(2533..>2795)
                     /locus_tag="PK198_25730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF42227.1"
                     /translation="NEDGTNDVLMIFADDNVLQIKSFRIFDRWGNMLYFNGPFPPNDP
                     QYGWNGEFEGRPMNSGVYVFSAEVEFVDGRTELLKGEVMLLR"
BASE COUNT          582 a          640 c          639 g          652 t
ORIGIN      
        1 ggtgaaagtg caagcctgtc ggtaacgacg gcttcatcgg ccattacctg gtcgaacggt
       61 gctacgggcg cagagctgac ggtgaccgag ggcggttggt acagcgcggt agtaggcacg
      121 ccgggctgcc aaaccatcac gcctgctatt gaatttacag tgaatccgcc ggtcttccct
      181 accatttctg tacagggcga cacgatattc tgtccgggcg gcagcgcgac cctgacggca
      241 agtccgtcct cttcatatac ttggtcgaat ggtgctgcca ctgcttccat agttgtgagt
      301 gcggcaggaa gctatacggt tgatgttcag gggctttgtg gtacctttac ttcgcagccg
      361 gtgaccatca gcttgttgga tgctcccctg cccgctgccg agaacgacac gatcacaggc
      421 gccggccagg ctgtattgac ggcaacgggc gaaatgcctc gctggtacga tgtgccctcc
      481 ggcggaacgc ccgtggctac cggcaatact tttaccattc ccatgctgac ggccacagat
      541 actttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagattatt ttcgacgcac
      661 accggtcttt cagattggcc actgtaaaag tgaataccga tatggccggc gttcgcatca
      721 tcgaattgcg tagcggcaat acggttctgg aggcccgtac cattgatatt cccgccggcg
      781 agtccgtcat tactttggat atgaacattc cgcaaggaaa tgacctcatt cttaccacca
      841 acactgctca gaaccagtcg gtattcggtt ttgccagtcc aagacttcgc cgcaccagtt
      901 cgggagtgat gtatccttat gtaacgccta atgtggtgac gctcaagact tccaacttcg
      961 gcaacgacgt ctattattat ttctacaatt gggaaatcaa gcagccggat acggaatnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aatgccgccc
     1081 aggaagcctg gctgggagac actcgtctga cgctttcgcc caatccgacg tccgaggcaa
     1141 ccgtattgaa cctctcacac ccgctgcatg aaacggccaa cctgaccctc accgacatga
     1201 acggtcgcat cctgagtcgc gcagttttac ctgccggtca gacacagtat cgttgggatg
     1261 tgcaggcatt gacgccgggc gtatatcact tgcgcatggc tacccgcgag ggtacggcag
     1321 tgagggcttt ggtagtaacg cgttgataat gagtttatgg ctgcgtattt tcatggtcgg
     1381 caattcgata cgttcgagtt tcatgccctc gaaaaagggg agtatgaagc ttgccggttt
     1441 accaatggga gcggccataa agcagacttg tcggagttca aattcattga ttgtgagttt
     1501 gttgtctgcg atttgagctt agcaaaactg aatcgaactg ctttcaggga agtgcagttc
     1561 cgggagtgta aactgtcggg actgcacttc gaaagctgcc atgatttcgg tctggctttc
     1621 gggtttgaac tatgccgtat agacttttgc accttcatcg ggatgaaact gaagggcact
     1681 cgtttcgtcc gttcccaatt gttggaatcc gattttacag attgcgatct caccggcgcg
     1741 ctctttgatc aaaccgatct cagccgggta gtttttgaca atactatttt ggaaaaggcc
     1801 gattttagaa ccgcctatgg ctatcaaata gaccccgaac aaaaccggat gcgtcaggca
     1861 aaattttcag cctctgggct ggcggggctg ctgggcaaat nnnnnnnnnn nnnnnnnnnn
     1921 nnnagataga atgagtgcta agatttgagc aactgcctca tttttttgtt acactctcat
     1981 ctgtttgaaa atcagttgca tgaagtctcc tttccgacca tccacatctc aaaatgcagg
     2041 ctggaacctc gccaagacag ccctgcaaac ctctgttttc tggttcattt ttctttatgt
     2101 tttccctcaa ctgatcctga aaatggaaag ggagctacaa tggccctcgt tttcatttcc
     2161 tgagtggatt gcctggcttt tattgttggt tttcagtgta ttgggtgttt atagcggata
     2221 tgccatgagc cgattcggac agggaacgcc actgccgacc gactgcgcca atgaattagt
     2281 tgtgcaaggg ccttacaaaa ttgtacgcaa tccgatggcc gtagcaggca tcgggcaggg
     2341 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgaa
     2461 aagccgattt ggcgatgctt acattttgta taaaaagcag gtaaagtgct ggattccaaa
     2521 attataaata gctcatcgca acaacatcac ttcgcctttc agtaactccg ttctgccgtc
     2581 aacaaactcc acctctgctg aaaaaacata gaccccggag ttcatcgggc ggccctcaaa
     2641 ctcgccgttc caaccatatt gcgggtcatt gggaggaaag ggaccgttga aatacagcat
     2701 gtttccccat cggtcgaaaa tgcggaatga tttgatttgc agtacgttgt cgtctgcaaa
     2761 aatcatcaat acatcgttgg tgccgtcctc gttcg
//
LOCUS       DAORLF010000550         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19708_length_2789_cov_2.233723, whole genome shotgun sequence.
ACCESSION   DAORLF010000550 DAORLF010000000
VERSION     DAORLF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19708_length_2789_cov_2.233723"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>311
                     /locus_tag="PK198_25735"
     CDS             31..>311
                     /locus_tag="PK198_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF42228.1"
                     /translation="MKNKTVPEAYVDDAVRRILRIKFRLGLFDDPYRYCDEEREKRIT
                     LSPAFMDVARDAGRKSIVLLKNEGNLLPLKTEAKRIAVIGPLAADKDAP"
     assembly_gap    312..361
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <362..1315
                     /locus_tag="PK198_25740"
     CDS             <362..1315
                     /locus_tag="PK198_25740"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRF42229.1"
                     /translation="LLEGVQAAAGPNTEILYAQGAALSVGLRTFLLPTTINTSDKSGF
                     AEALAAARKAEVVVLAIGEDCWQSGEGRSVANLELSGLQMELFNEIYRINKNVVVVLM
                     NGRPLAIQELAGKAPAIVEAWHLGSQAGHAIADVLFGKYNPSGKLPVSFPRVTGQVPI
                     YYNYKSTGRSYPSKPNEVTWSHHNDVPNSPLFPFGFGLSYTTFSYSDIALSAKEMTAA
                     APLQVSVTVTNSGKAAGTETVQLYVQDLVGSVTRPVKELKGFQKITLAPGESKKVTFT
                     ITAKDLEFYTAARKWEAERGAFKVFVGTNSAEVKEAGFVLK"
     gene            1324..1962
                     /locus_tag="PK198_25745"
     CDS             1324..1962
                     /locus_tag="PK198_25745"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF42230.1"
                     /translation="MNSSQNFINRHFPFLPEPLRLEMETDGVLKEFPAGTELLREGQY
                     VKVIPIVFDGLVKVMGRFDDKDLLLYYIRPKESCIMSFSAVLDNTPSKILAITEEATT
                     ALLLPSPSVEKWVRDFPAFNRLFYQQFNQRYEDMLQTIRALFYERMDQRLLSYLRQKA
                     ALKGNAVMTLRHWEIASDLGSAREVISRLLKKLETEGLIRQLPHGQIEVCKW"
     gene            2041..2256
                     /locus_tag="PK198_25750"
     CDS             2041..2256
                     /locus_tag="PK198_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRF42231.1"
                     /translation="MKKNMGSMDRLIRVIIAAAVAVLYFTGTITGTLGIVLLVLAGVF
                     VATSLISFCPLYAPFGLNTCSTKERKG"
     gene            complement(2456..>2789)
                     /locus_tag="PK198_25755"
     CDS             complement(2456..>2789)
                     /locus_tag="PK198_25755"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRF42232.1"
                     /translation="VFVSVGIFFLLLNLVTYEGEMWFFFPLLPWMVGVLIHYFTNFGF
                     PGTEIMNEGWEERQLEKEVRDILQKEGRLRRTVSEHSGELPPAQEGLELPDLEKTKEK
                     QWSDRDFV"
BASE COUNT          643 a          747 c          709 g          640 t
ORIGIN      
        1 cgtgcttata ttgttcactt ggccaattgg gtgaaaaaca aaaccgtacc ggaagcctac
       61 gttgacgacg ccgtgcgccg catcctccgc atcaaattcc gcctcggcct ttttgacgac
      121 ccctaccgct actgtgatga agaacgcgaa aaacgcatca cgctcagccc ggctttcatg
      181 gatgtagccc gcgacgccgg ccgcaaatcc atcgtcttac tcaaaaatga aggcaacttg
      241 ttacctctca aaaccgaggc taaacgcatc gccgtcatcg gcccattggc cgccgacaaa
      301 gatgctccat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nttgctggaa ggcgttcagg ctgctgccgg gccgaatacc gaaatacttt acgctcaagg
      421 cgcggcattg tctgtcggtt tgcgtacttt tctgcttcca accaccatca atacgagcga
      481 caaatcgggg tttgccgaag cccttgcagc cgcccgcaaa gccgaggtgg tggtattggc
      541 catcggtgag gattgctggc aaagcggaga ggggcgcagc gtagccaatc tggaattatc
      601 gggcttgcaa atggagctgt tcaacgaaat ttaccgcatc aataaaaatg tggtagtggt
      661 actgatgaac ggccgtcctc ttgccattca ggaattggcc ggcaaagcgc ccgccattgt
      721 ggaagcctgg cacctcggtt cgcaggcagg ccacgccatc gccgacgtgc tgtttggcaa
      781 gtacaatccc agcggtaaat tgccggtgtc gtttccgcgc gtgacgggtc aggttcctat
      841 ttattacaat tacaaaagca ccgggcgaag ctacccttcg aagcccaatg aggttacctg
      901 gtcgcaccac aacgacgtgc ccaattcgcc gctgtttcca tttggcttcg ggctgagcta
      961 caccaccttc tcctactccg acattgccct cagcgccaaa gaaatgactg ctgctgcacc
     1021 tttgcaggtc agcgtcacgg tgacgaactc aggtaaggcc gccggtacgg aaacggtgca
     1081 actttatgtg caggacctcg tgggcagcgt cacccggcca gtgaaggaac tgaaaggctt
     1141 ccaaaagatt acacttgcgc ccggtgaatc gaaaaaggtc acgtttacca tcaccgccaa
     1201 agacctggag ttttacaccg ccgcccgcaa atgggaggcc gaacgggggg ccttcaaagt
     1261 gtttgtcgga actaattctg ccgaggtaaa agaggccggt tttgtgctga aatgatcata
     1321 tccatgaatt cctcccagaa tttcatcaac cgccactttc cctttctccc ggagccgctg
     1381 cgcttagaga tggagacaga cggtgtgctg aaggagtttc cggcaggaac ggaattgctc
     1441 cgcgaaggcc agtacgtcaa ggtaattccc attgtgtttg acggtttggt caaggtcatg
     1501 gggcgtttcg acgacaaaga cctgctgctc tactacatcc ggcccaaaga aagttgcatc
     1561 atgtcgtttt cagcggtatt ggacaatacg ccgagcaaaa tattggccat cacagaggaa
     1621 gccacgactg cgctgttgct gcccagtcct tccgttgaaa aatgggtgcg cgattttccg
     1681 gcattcaacc ggctgtttta ccaacagttc aatcagagat acgaggacat gctgcaaacg
     1741 attcgcgctc tgttttacga acgcatggat cagcggctgt tgtcgtatct tcgccaaaag
     1801 gcagccctca aaggcaacgc tgtgatgacc ctgcgacatt gggaaatcgc ttccgatctg
     1861 ggcagtgcga gggaggtcat ttccaggctg ttgaaaaaat tggaaacgga ggggcttatc
     1921 cggcaactcc cgcacgggca aattgaagtg tgcaagtggt gaccgcagtc accgactgag
     1981 ggtcgggcgg gggcgtatct ttgcatcgtg attgattttt taaccaaaat tcgccttgcc
     2041 atgaaaaaga atatgggttc tatggatcgc ctgatccgcg tcatcattgc agcggcggta
     2101 gctgtgttgt atttcaccgg aacgattacc ggcacgctgg gtattgttct tctcgtactg
     2161 gccggcgttt tcgtcgccac cagcctcatc agtttctgcc cgctgtacgc tccgttcggt
     2221 ctgaacacct gctcgacgaa ggagagaaag gggtaggttt cctgcctttt tctaatagaa
     2281 agggcaattt cctcttatcg ggaagttgcc ctttattatc gtaagtggtc taccagcagg
     2341 ctgtccccgc cgccgatgtc tatgatgcgg gcggttttag caactttgta attttgaaaa
     2401 aatcaagcga agtccaagtt agaaccgcct accgcaaact gcctactgag aaccgctaca
     2461 caaaatccct atcgctccac tgcttttctt tggtcttttc cagatcgggc agttccaatc
     2521 cttcctgagc cgggggcaat tcgccggaat gctccgaaac cgtcctgcgc aggcggcctt
     2581 ctttttggag tatgtcgcgt acctcttttt ccaactggcg ttcttcccaa ccctcgttca
     2641 tgatttccgt tcccggaaaa ccgaagttgg tgaagtaatg gatcaatacg cccaccatcc
     2701 agggcagcag cggaaagaaa aaccacatct cgccttcata ggtaacgagg ttgagcagca
     2761 aaaagaaaat gcccaccgac acaaagact
//
LOCUS       DAORLF010000551         2788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19715_length_2788_cov_2.111965, whole genome shotgun sequence.
ACCESSION   DAORLF010000551 DAORLF010000000
VERSION     DAORLF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19715_length_2788_cov_2.111965"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PK198_25760"
     CDS             <1..1080
                     /locus_tag="PK198_25760"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42233.1"
                     /translation="VDMLLTGFDSKYLNTLYVDKNLRHHGLIQAFSRTNRILNGSKPS
                     GNILDFRRQQQEVDEAVALFSGEDTGRAKEIWLTEPAPKVIEKFEKAVAAMAQFMAQN
                     HLVNEPHEVYNIKGDTARIEFINRFKEVQRLKTQLDQYTDLSEAQKESIESIIEEDQL
                     RSFRSAYIETAKKFKALQQKDSPNTPPDVQQLDLELVLFASTIIDYDYIMALIAKYTQ
                     NKPAKQRMTRGQLVNMLGSSANLMDERDDIVAYIDSLEAGKPLTEPEIKDGFQIFKSE
                     KFAQELSAIAQKHGLEPPALQAFVDGILSRMIFDGEQLGDLLAPLELGWKARAQKELA
                     LMEDLVPLLKKRAQGREISGLKAYE"
     gene            1073..>1459
                     /locus_tag="PK198_25765"
     CDS             1073..>1459
                     /locus_tag="PK198_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HRF42234.1"
                     /translation="MSKEIAHPAQPVFDELLQHIQQARSKAFRQANTVLIDLYWRVGK
                     TISEKVQTEVWGKGVVAELARYIARQAPDIRGFSDKNLWRMKQFYETYREDEKLATLW
                     RELSWSHNRLIMTCKHPEERAFYLQLS"
     assembly_gap    1460..1484
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1485..2120
                     /locus_tag="PK198_25770"
     CDS             <1485..2120
                     /locus_tag="PK198_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRF42235.1"
                     /translation="QEKWSVRALERQINTAQFERTLLEGPKLSPLLRVLHPDAMQVFK
                     DNYILEFLGLPDMHSENDLQKALVQHMKQFILELGPDFIFMGEQYRLQVGNQDFYLDL
                     LFYHRGLSALVAFELKIGKFLPKHLGQLNFYLEALDRDVKKPHENPSIGVLLCRDKDE
                     EVVEYALSRNLSPALIAQYQLQLPDKKIIQAKLHEWMQQLPDMNTTQNNNA"
     gene            2117..>2788
                     /locus_tag="PK198_25775"
     CDS             2117..>2788
                     /locus_tag="PK198_25775"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRF42236.1"
                     /translation="MSKEMNNTKTPRLRFAEFREAWTKKTLGGIAVLINDKAGINRYK
                     LMSITAGIGLVSQIEKFGREIAGESYKNYYVIKKGDFAYNKSSTKQYPEGQIVVLENE
                     ETGAVPNSIFTCFRIDDKLISPFFLKYPFENNIHGNWLKKFIEVGARAHGALSVDTKD
                     LFATPIFFPSLAEQQKIAAFLTSLDELIAAEGQKLKALQAHKKGLMQQLFPAEGETTP
                     RRRFGG"
BASE COUNT          812 a          714 c          670 g          567 t
ORIGIN      
        1 gtggatatgc tgctcaccgg cttcgactcc aaatacctca acaccttgta tgtggacaaa
       61 aacctgaggc atcacggctt gatacaggct ttttcgcgca ccaaccgcat cctcaacggc
      121 agcaaaccca gcggcaatat cctcgacttc cgccggcaac agcaggaagt ggacgaggct
      181 gtagccctgt tctctggcga agataccgga cgggccaaag aaatctggct gacggagcct
      241 gcccccaaag tgattgaaaa atttgaaaaa gccgtggcag ccatggcgca gttcatggcg
      301 caaaaccatc tcgtgaacga gccgcacgag gtttacaaca tcaaaggcga cacggcccgc
      361 atcgaattca tcaaccgatt caaagaggtg caacggctga aaacacagct cgaccaatac
      421 accgacctga gcgaggccca aaaggaaagc attgaatcca tcatagagga agaccaactg
      481 cggtctttcc ggagcgcgta catcgaaacg gccaaaaaat tcaaagcctt gcagcaaaaa
      541 gacagcccca acacgccgcc cgacgtgcag caattggatt tagaactggt cttgtttgcc
      601 tctaccatca ttgactacga ttatatcatg gcgctgattg ccaaatacac ccaaaacaaa
      661 cccgccaaac agcgtatgac gcggggacaa ttggtgaaca tgcttggttc cagcgccaac
      721 ctgatggacg agcgcgacga tattgtggcg tatattgaca gcctcgaagc gggcaaaccg
      781 ctcaccgaac cagagatcaa agacggcttc caaatcttca aatccgaaaa atttgcccag
      841 gaactgtcgg ccatcgccca aaagcacggc ctggaaccgc ctgcattaca agcctttgtg
      901 gacggcatcc tgagccgcat gatttttgac ggcgaacaac tcggcgacct gctggccccg
      961 ctcgaactgg gctggaaggc ccgcgcacaa aaggaattag cattgatgga agacctggta
     1021 ccacttttga aaaagcgcgc ccaggggcgc gaaatatccg gattgaaagc ctatgagtaa
     1081 ggaaatagcc catcccgccc aacctgtttt tgacgagctg ttgcagcaca tccaacaagc
     1141 gcgctccaaa gctttccggc aagccaatac ggtactgata gatctgtact ggcgcgtggg
     1201 caaaaccatc agcgaaaagg tacagaccga agtctggggc aaaggcgtag tggccgaact
     1261 ggcacgctac attgcccggc aggcgcccga catcagaggc ttcagcgaca aaaacctctg
     1321 gcgcatgaag cagttttatg aaacgtatcg ggaggatgaa aaactcgcca cgctgtggcg
     1381 agaattgtca tggagtcata atcgcctcat tatgacgtgc aagcatccgg aggaacgagc
     1441 gttttatctt caactttctn nnnnnnnnnn nnnnnnnnnn nnnncaggaa aaatggagtg
     1501 tgagggcgtt ggagcgccaa ataaataccg cacaatttga gcgtacgctt ctcgaaggcc
     1561 caaaactctc accactgttg agagttttgc acccggacgc aatgcaggta tttaaagaca
     1621 actacatact ggaatttttg ggcctgcccg acatgcacag cgagaacgac ttgcagaaag
     1681 ccctggtgca gcacatgaaa cagtttatcc tggaactggg ccccgacttc atttttatgg
     1741 gcgaacagta ccgcctgcaa gtgggcaatc aggactttta cctcgatctc ttgttctacc
     1801 accgcggcct cagcgcctta gtggccttcg aactcaaaat cggcaaattt ttgcccaaac
     1861 acctgggtca actcaacttc tacctcgaag cattggacag agacgtgaaa aaaccgcacg
     1921 aaaaccccag catcggcgta ttgttgtgcc gcgacaaaga cgaggaagtg gtggaatatg
     1981 ccctctcgcg caacctctcg cccgccctca tcgctcaata ccaactgcaa ctgcccgata
     2041 aaaaaatcat tcaggccaaa ctgcacgaat ggatgcaaca actacccgac atgaatacca
     2101 cgcaaaacaa caacgcatga gcaaggaaat gaacaatacg aagacgccac ggctgcggtt
     2161 tgcggagttt cgggaggctt ggactaagaa gacgctaggc ggcatagctg ttttgattaa
     2221 tgacaaagca ggtataaata ggtataaatt gatgagcatc acagcgggaa ttggtcttgt
     2281 atctcaaatt gaaaaatttg gtagagaaat agctggtgaa tcatacaaga actattatgt
     2341 gataaaaaaa ggggattttg cttacaataa aagctcaaca aagcaatatc cagaaggtca
     2401 gatagtggtt ttagagaatg aagaaactgg ggcagttcca aatagtattt ttacttgttt
     2461 ccgtattgat gataaactta tctcgccctt ttttcttaaa tatccatttg aaaataacat
     2521 tcatggtaat tggcttaaaa agtttattga agtaggtgcg cgtgctcacg gcgctttaag
     2581 cgtggataca aaagatttat ttgctacccc gatatttttc cctagcctcg ccgaacagca
     2641 aaaaatcgcc gctttcctca cctccctcga cgagctcatc gccgccgagg gccaaaagct
     2701 gaaagccctg caagcccata aaaaagggct gatgcagcag cttttcccgg cagaagggga
     2761 gacgaccccg aggcggaggt ttggaggg
//
LOCUS       DAORLF010000552         2784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19760_length_2784_cov_2.074753, whole genome shotgun sequence.
ACCESSION   DAORLF010000552 DAORLF010000000
VERSION     DAORLF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19760_length_2784_cov_2.074753"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>671)
                     /locus_tag="PK198_25780"
     CDS             complement(<1..>671)
                     /locus_tag="PK198_25780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42237.1"
                     /translation="ALQWRILANLNLSFAGISAPFSLAFSDANRQFNLPSYTFTGISP
                     TYKWARLHLGDRSLNFSKYTLNSINFRGAGFELQPGRLYVAGMYGRLRRAVAEDLLSQ
                     QMLEPAYLRTGYGFKAGITGVNSEYHIMVFGAWDDPNSITQPLMRAVLPANNAVVSLN
                     GRQQLGKKFNAEIELARSAYNQDRRLDPLATNERKAMNTLLGLLQPTVSLFNGNAVRG
                     KLAYNT"
     assembly_gap    672..703
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(965..>2784)
                     /locus_tag="PK198_25785"
     CDS             complement(965..>2784)
                     /locus_tag="PK198_25785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF42238.1"
                     /translation="SDWSAETYKDYTGIPAGLYRFRVRAKNLYDLVGEAAEFEFRVLP
                     PFYLSVWAWLCYALLFAGLVWLIWKLQMKQVEKRHILKIKQLEFEKLKELDQLKSRFF
                     ADISHEFRTPLTLILGPLEQLSGEEKRPAIVRHYQVMESNARRLLRLINQLLDLSRLE
                     AGKMQLDMREADVLPLLKGCAFAFESLSMSKNIRLQVQCDIPGAVLPFDRDKMEQVFT
                     NLISNALKFTPEGGAVQVRAFEAAQKSVLRIEVSDTGVGIAEEQLPLVFERFFQSDAT
                     SRLHASGSGIGLALTKELTQLHGGHIGVDSRPGQGSVFFIELPVRSFTAEEAASEKPV
                     SAVETLLPPSLPTESGTPELNAENTLLLVEDNPDMRVFIRDILADKFRVIEAADGQEG
                     LEKALEHIPDLIVSDVMMPRMDGLELCDALKNDERSSHIPIVLLTAKADLDSRIAGLR
                     RGADDYLAKPFHREELLLRAGNLLLQRQRLRERYASLEPLPAAVPDPGIEIEDAFLLK
                     IRHLAEPRLTDADFDIDQLAKLAGMSRSQMFRKIKALTGKSPSVFIRDIRLQRAQELL
                     RTTTKNVTEIAYEVGFSTPAYFSDAFVEAFGVRPSQFKA"
BASE COUNT          555 a          752 c          829 g          616 t
ORIGIN      
        1 gtgttgtacg ccaatttgcc gcgcacagcg ttaccgttga agagcgacac ggtgggttgc
       61 aacagtccca gcagggtgtt catggccttg cgttcgttgg tagcgagcgg gtccaagcgc
      121 cggtcctgat tgtaggcgct gcgggccaat tctatctctg cgttgaattt tttgcccaat
      181 tgctgccgcc cgttcaggct caccacggca ttgttggccg ggagtaccgc gcgcatcagc
      241 ggctgcgtga tggagttggg atcgtcccag gcgccgaaca ccataatatg atactcgctg
      301 ttgacgcccg taatgcccgc tttgaatccg tagccggtgc gcagataggc gggttccagc
      361 atttgttggc tcagcagatc ttcggccacc gccctgcgca ggcgtccgta catgcccgcc
      421 acatacagcc gtccgggttg caattcgaag ccggcgcccc ggaagttgat ggagttgagg
      481 gtgtatttgg aaaaattgag actgcggtcg ccgaggtgca ggcgcgccca tttgtaggta
      541 ggactgatgc cggtgaaggt gtagctgggc aggttgaact gccggttggc atcggaaaag
      601 gccagcgaaa acggcgcgga aatacctgca aaactgaggt tgagattggc taagatgcgc
      661 cattgcagtg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgtgta aaagttattg
      721 gccagattca gcccgccggt aatctggagc ggcccccgga acgattgccc gatttgggaa
      781 acatcctgcc cggctgccgg catggccatc atggtcagga atatgaaaac cggccaggcg
      841 atttttcgtg tgttcaagta ctgcatacgc tacgatggat tggtgtcctt tcggtagcaa
      901 atgtactttc acaggccctc ggcgggcttc caataattgg tcaaaaatca ctgattatgt
      961 tgcatcaagc cttgaattga ctcggccgca ccccaaacgc ctccacaaac gcatccgaaa
     1021 aatacgccgg cgtagagaag cccacttcgt aggctatttc ggtcacgttt ttggttgtcg
     1081 ttcggaggag ttcctgggcg cgttgcaggc ggatgtcgcg gataaaaacg gagggcgact
     1141 tgccggtgag ggctttgatt tttctgaaca tctggctgcg actcatgccc gccagcttgg
     1201 ccaattggtc tatgtcgaaa tcggcgtcgg tcaggcgggg ttcggccaag tggcgtattt
     1261 tcaacaaaaa agcgtcctct atctcgatgc ctgggtcggg tactgccgcc ggcagcggct
     1321 ccaatgatgc gtatcgctcg cgcaggcgtt gccgttggag gaggagattg cccgcacgca
     1381 gcaacaactc ttcgcggtgg aatggcttgg ccaagtagtc gtcggcgccc cggcggagac
     1441 ccgcaatgcg cgaatcgagg tcggctttgg cggtgagcag gacgatggga atgtggctgc
     1501 tgcgctcgtc gtttttcagg gcgtcgcaga gttccagccc gtccatgcgg ggcatcatca
     1561 cgtcgctgac aatgaggtcg gggatgtgtt ccaatgcttt ttcgaggccc tcctgcccgt
     1621 cggcggcttc gatgacgcga aacttgtcgg cgagtatgtc ccgaatgaaa acgcgcatgt
     1681 cggggttgtc ttccaccaac agcagggtgt tttcggcgtt gagttcgggc gtgccggatt
     1741 cggtcggcaa ggagggcggc aggagcgttt cgaccgcgct taccggcttt tcggaggcag
     1801 cttcttcggc ggtaaatgag cgcaccggca gttctataaa aaagacagaa ccctggcccg
     1861 gcctgctgtc cacgccgatg tgtccgccgt gcagttgagt cagttctttg gtgagcgcca
     1921 gcccgatgcc gctgcccgaa gcatgaagcc gggaagtggc atcgctttgg aaaaaacgct
     1981 cgaaaacgag gggcaactgc tcctctgcaa tgccgacgcc ggtatcgctc acctcaatgc
     2041 ggagcacaga cttctgcgcg gcttcaaaag cgcgtacctg tacggcacca ccttcgggcg
     2101 tgaatttcaa ggcattggaa atcagattgg taaatacctg ctccatctta tcccggtcaa
     2161 aaggcagcac cgctccggga atgtcgcatt gcacctgcaa gcggatgttt ttgctcatag
     2221 acagcgattc gaaggcaaaa gcgcagcctt tcagcagggg cagcacatcg gcctcccgca
     2281 tgtccaattg cattttgccg gcctccagcc gcgacaggtc gagcagttga ttgatgagcc
     2341 gcagcagccg ccgggcgttg gattccatca cctgatagtg ccgcacgatg gcggggcgtt
     2401 tttcttctcc gctcagttgt tccagcgggc cgaggataag ggtgagcggg gtgcgaaatt
     2461 cgtgcgatat gtctgcaaaa aagcgcgact tgagctggtc caattctttg agtttttcaa
     2521 actccaactg cttgattttc agtatatgtc ttttctctac ctgcttcatt tgcagcttcc
     2581 atatcagcca taccaagccg gcaaacagca gcgcgtagca cagccatgcc cagacgctga
     2641 ggtaaaacgg cggcagcacc cgaaactcaa attcggcggc ctcgcccacc agatcgtaca
     2701 ggtttttggc gcgcactctg aaccggtaaa gacccgccgg aatgcccgtg tagtctttgt
     2761 aggtctcggc gctccagtcc gacc
//
LOCUS       DAORLF010000553         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_19793_length_2781_cov_1.917461, whole genome shotgun sequence.
ACCESSION   DAORLF010000553 DAORLF010000000
VERSION     DAORLF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19793_length_2781_cov_1.917461"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>222
                     /locus_tag="PK198_25790"
     CDS             <1..>222
                     /locus_tag="PK198_25790"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF42239.1"
                     /translation="PVPYANIVVKDKYRGTYTDLKGFFSIVVQKGDVIVFSAIGYKDA
                     QYHIPHDLTDDRYSIVQLMTQDTINLPET"
     assembly_gap    223..300
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <301..524
                     /locus_tag="PK198_25795"
     CDS             <301..524
                     /locus_tag="PK198_25795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF42240.1"
                     /translation="RIAAANMANEALSRMRNEVPRDAAETGSYYLRQQASQYYSIGQA
                     PPMNIFNAAAWKRFFDAWKRGDFKKKNGD"
     gene            549..1247
                     /locus_tag="PK198_25800"
     CDS             549..1247
                     /locus_tag="PK198_25800"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRF42241.1"
                     /translation="MKYFTIALLLCGFSSVFSQSNKHGFELWGAYISSVQLSEKYALW
                     NDFHLVTNSFFISRHGLTRQLGKDLSISGGYAWLRTATSFSDRLIRQEHRPWLQVEWA
                     VRLSPKNRYRLRLRYDSRFRKRIEGMACGDDFIHQNRLRVMNSLRFQIREMGEGKSLH
                     FNVMNETLFHFGQGIDGLRLDQNRSYLLGGVSIANTTVMGGCHLRVLPGTTSTNFQHG
                     ITMWVVQRFGLPKK"
     gene            complement(1253..1975)
                     /gene="recO"
                     /locus_tag="PK198_25805"
     CDS             complement(1253..1975)
                     /gene="recO"
                     /locus_tag="PK198_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRF42242.1"
                     /translation="MLVKTSGIVFRAVKYSETSIITDVYTRDLGMQTYIVNGVRSARS
                     KTGAALFQVMSLLEVVVYHRDDRKMHRVKEIRAAHVYQRLPFDVRRSAVGMFMAEIGR
                     KTIREPEPNAPLFDFLFDSFCFLDSTQEPFANLHLHFLIGLSAHLGFLPGGDFTPETP
                     VFDMQEGTFTHSATQIHCMDEEQSWLLYQLLCCEREQCHIVVLQHAQRRRLLQSLLTF
                     YRLHIESLPEIRSHEILHEVFE"
     gene            complement(2107..2391)
                     /locus_tag="PK198_25810"
     CDS             complement(2107..2391)
                     /locus_tag="PK198_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42243.1"
                     /translation="MVQARRYVIQNRELAHRCIVAAQHMMHLGLEEAHVIFVIAASNG
                     GAVGLPPIVQRKRAVLTNVGIIEIHHNERHACKKRSAVQIAVEFVGFHVL"
     gene            2462..>2781
                     /locus_tag="PK198_25815"
     CDS             2462..>2781
                     /locus_tag="PK198_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42244.1"
                     /translation="MLLRWLGSPFLSVALPLAFYGSPLLAFYGFNFLPNPAALGLGLV
                     GAYGLMRYWENPNRDRLLFAAAFLLLGGLLKVSLLIIPIAAIGVAKWLYFVGDREKYF
                     PDKKA"
BASE COUNT          603 a          736 c          711 g          653 t
ORIGIN      
        1 accctgttcc ttacgccaat attgtggtaa aagacaaata cagaggcact tacaccgatc
       61 tcaaaggctt cttctccatt gtggtgcaaa aaggagacgt catcgttttt tccgccatcg
      121 gctacaaaga cgcccaatac cacatcccgc acgatctcac cgacgaccgc tactccatcg
      181 tacagctcat gacacaggat accatcaacc tgcccgaaac ggnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 aaagaattgc cgccgccaat atggccaacg aagccctcag ccgtatgcgc aacgaagtgc
      361 cccgcgatgc cgctgaaacc ggcagctact acctccgcca gcaggccagc cagtactact
      421 ccatcggtca ggcgccgccc atgaacatct tcaacgccgc cgcctggaag cgcttcttcg
      481 acgcctggaa acgcggcgat ttcaaaaaga aaaatggaga ctaaagggct ggtcgtttac
      541 ctctcattat gaaatatttt acaatagcac tgcttttgtg cggcttttct tctgtctttt
      601 cgcaaagcaa caagcacggc ttcgaattgt ggggcgctta tatctcatct gttcagttga
      661 gtgaaaaata cgccttatgg aatgattttc atttggttac caattccttt tttatttccc
      721 ggcacggtct cacccggcag ctcggcaaag acctcagcat cagtggcggt tatgcctggt
      781 tgaggactgc cacctcgttt tcagaccggc tcatccggca ggaacaccgg ccctggttac
      841 aggtggaatg ggctgtccgg ctttcgccca aaaaccgcta tcgcctccgc ctgcgctacg
      901 acagccgctt ccgcaaacgc atcgaaggca tggcttgcgg cgatgacttt attcaccaaa
      961 accgactgcg ggtgatgaac agcctgcgct tccagatcag agagatgggg gagggtaagt
     1021 cgctgcattt caatgtaatg aacgagacct tgtttcattt cgggcagggc atagatggtt
     1081 tgcgtttaga ccaaaaccgg agctacctgc tcggcggggt cagcatagct aataccaccg
     1141 tgatgggcgg ctgtcatctg cgcgtattgc cgggaaccac ctctaccaac tttcagcacg
     1201 gcattactat gtgggtggtg cagcgtttcg ggcttcctaa aaaatagggt atttactcaa
     1261 acacctcgtg caaaatctcg tgcgagcgta tttccggcag gctttcgatg tgcaaccggt
     1321 agaacgtgag cagcgattgc agtaagcgtc ggcgttgggc atgttgcagg acaacgatgt
     1381 gacactgctc gcgctcgcag cacaacaact ggtacaacag ccaactttgc tcctcatcca
     1441 tgcagtgtat ctgggtggcg ctgtgcgtga aagtgccttc ctgcatatcg aaaacaggcg
     1501 tttcgggggt aaaatcgccg ccgggcagga accctaaatg agcgctcaat ccgatcagga
     1561 aatgcaggtg cagattggca aacggctcct gtgtgctgtc taaaaagcaa aaagaatcga
     1621 acaaaaagtc gaacagcggc gcgttaggct ccggttcgcg gatggttttg cgccctattt
     1681 cggccataaa catgcccact gcgctgcggc gcacatcgaa aggcaggcgt tgatacacat
     1741 gcgccgcccg aatttccttc acgcggtgca tcttgcggtc atcgcggtga tacaccacca
     1801 cctccagaag cgacatgacc tgaaacagtg ctgcgccggt tttgctgcgc gcgctgcgca
     1861 ctccgttgac gatatacgtc tgcataccca gatcacgggt atagacgtcg gtgatgatac
     1921 tcgtttcaga gtattttacc gctctaaaaa caatgccgct ggttttgaca agcatgtcgg
     1981 ttcgatggct ttaaaagctg atgtgccgga cggcctgcaa aagtaataga tttgcgcatg
     2041 tgtcgggtct atgcttttgg cggccacacg ttgcatactc acaaaaaacg ctatctttcg
     2101 cccaatttaa agcacatgga aaccaacaaa ctctacggct atctgcacag cgctgcgttt
     2161 tttgcaggcg tgacgctcat tgtggtgtat ttcaattatg ccgacattag tcagtacggc
     2221 cctttttcgc tgcacaattg gcggcaagcc gacggcgcct ccattgctcg ctgctattac
     2281 gaaaatgaca tgcgcttctt ccagcccaag gtgcatcatg tgctgggcgg cgacaatgca
     2341 gcggtgggcg agttcccgat tttgtattac atagcggcgg gcctgtacca ctttttcggg
     2401 ccgcacgacg tcattttgcg gctgctggtt ttcggctgct ttacggcggg catggtccaa
     2461 gatgttgttg aggtggttgg gcagcccgtt tttgtcggta gcattgcctt tggcctttta
     2521 cggttcgccg ctgctggcct tttacgggtt caatttcctg cccaatccgg cggcgttggg
     2581 cttaggtttg gtgggcgcct acggcctgat gcgttattgg gaaaacccca accgcgaccg
     2641 gctgctgttt gcggccgcat tcctgctgct gggtgggctg ctcaaggtca gtttgctcat
     2701 catacccata gcggccatag gggtggcaaa gtggctgtat tttgtgggcg accgcgagaa
     2761 gtatttcccc gataaaaagg c
//
LOCUS       DAORLF010000554         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20369_length_2716_cov_2.202555, whole genome shotgun sequence.
ACCESSION   DAORLF010000554 DAORLF010000000
VERSION     DAORLF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20369_length_2716_cov_2.202555"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK198_25820"
     CDS             complement(<1..251)
                     /locus_tag="PK198_25820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRF42245.1"
                     /translation="MATNFQPSSGGLQAGKALVVLLVIVLFSIVLYKKVFKKVADYTL
                     VPKEMQINYLPADYKLNVDEEDALAILSNPHRYRREFNDL"
     gene            438..1295
                     /gene="folP"
                     /locus_tag="PK198_25825"
     CDS             438..1295
                     /gene="folP"
                     /locus_tag="PK198_25825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRF42246.1"
                     /translation="MFAVRRTLNCKGRLLSLERPIVMGILNTSPDSFYDGGRYQSLDA
                     ALRQTEQMLSEGAAIIDVGGMSSRPGAAQISIQEELRRVLPVIEAIAGRFPEAFISID
                     TVHAQTAREAVAAGACIVNDISAGRLTEGMYETVAELGVPYVLMHMQGRPETMQQQAQ
                     YDDVVQEVLDFLIAEVGRLQAAGVKDILLDPGFGFGKTMEHNFQLLQNLHVLGILPYP
                     VLAGISRKSMIYRSLGISPKEALNGTTALHIVALQQGALLLRVHDVAEAVQVVRLWEQ
                     LQSAQYSPA"
     gene            1292..>2716
                     /locus_tag="PK198_25830"
     CDS             1292..>2716
                     /locus_tag="PK198_25830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42247.1"
                     /translation="MNEVTAIQPLSEQEIKDIMLRFLKRHYRNRQRNGETEIASDARG
                     KGGILADGYLSFAKPGGGWFVATFEATSYDTRQEVRFTQRRGLLFWDNMVAALWGTAI
                     GGGFNITQQWLPIKKYGPALPLVMLISVFTGLFFLVRMLSSHWRRYRQIYAVEQFKRY
                     HTDEQWIAVAEEVFNGPHDPHFIELRRQCIRYGFGLVVIAANEAPRAHLTPAREDVFE
                     GRRDIVKLITDNELVKNMQNLGRQAWITELWGKMSAYIPQSDQWLHRFGGRLPKHWLA
                     SAVAFVFIIAIWYHDYDTWAHRKVTPKVYAEEAIQALRALPPEPSTINLDTPYFWPAP
                     FLDDRISYLDEWLEDRLIAQRLFRNSARNAAAREDGYGVIFYDCARFLNFEGVKFLLL
                     DGVYSDFESSLLRVEMLQADGIQAGSLWLGCFDERSKEYAVYLGPLFNTPGEAGRLAR
                     ELEYLRSDRSSRDFLTVKAIKADAS"
BASE COUNT          588 a          786 c          743 g          599 t
ORIGIN      
        1 agatcgttga actcgcggcg atagcggtgc ggattggaaa gaatggccag cgcgtcttcc
       61 tcgtccacgt tgagtttgta atcggcaggc aggtagttga tctgcatttc tttgggaacc
      121 agcgtgtaat cagccacttt tttgaacact tttttgtaca aaacaatgct gaacagcacg
      181 atgacgagca gcaccaccag cgctttgccg gcctgcaagc cgcccgatga aggttgaaaa
      241 ttagttgcca ttatttgaat agttggtttt tgaatatgtt tcgtgtttcg cgttcaaggt
      301 tttggggagc agtgaagggg tgactcagcg agtttcagtt gggtagtcac cccttcacta
      361 agctggacca aaaaccgccc tttgccgaaa atctgcttac ctttggcctg caaatgtaac
      421 cacaaaaccg gtaccgaatg ttcgccgttc gtcgcacgct caattgcaaa ggccgtttgt
      481 tgtcgttgga acgccccatc gtgatgggca ttctcaacac aagccccgat tccttttacg
      541 acggcgggag gtaccagagc ttagacgcag ccctgcgcca aacagaacaa atgctctccg
      601 aaggcgccgc catcattgac gtaggaggca tgtcttcgag gcccggagca gcgcaaattt
      661 ccatacagga agagctgcgc cgcgtgctgc ccgtcatcga agccatagcc ggccggtttc
      721 ccgaagcctt catttccata gataccgttc acgcccaaac cgcgcgcgag gccgtagctg
      781 ccggggcctg catcgtgaac gacatttctg ccggacgcct caccgaaggc atgtacgaaa
      841 cggtggcgga actcggcgtg ccttacgtcc tgatgcacat gcagggccgc cccgaaacca
      901 tgcaacaaca ggcgcagtac gacgatgtgg tgcaggaggt actcgatttt ttgattgccg
      961 aggtaggccg cttgcaggct gccggcgtca aagacatcct cctcgatcca ggcttcggtt
     1021 ttggcaaaac catggaacac aatttccaac tgcttcaaaa cctgcacgtt ctgggcattc
     1081 tcccctaccc tgttttggcc ggcatttccc gcaaatccat gatataccgc agcctcggca
     1141 tctcaccgaa agaggctctc aacggtacca cggcactgca catcgttgcc ttgcagcagg
     1201 gcgcgctgct gctgcgcgta cacgacgtag ccgaggcggt gcaggtcgtt cggctctggg
     1261 aacaactcca atctgcacaa tacagccccg catgaacgaa gtcaccgcca tccagccatt
     1321 gagcgagcag gagatcaagg acatcatgct gcgcttcctg aaacggcact accgcaaccg
     1381 gcagcgcaac ggcgaaacgg aaatcgcctc cgacgcgcgc ggcaaaggcg gtattctggc
     1441 cgacggctac ctcagctttg ccaagcccgg cggcggctgg tttgtagcca cttttgaagc
     1501 cacttcttac gatacccggc aggaagtgcg ctttacgcaa aggcgcggat tgctgttttg
     1561 ggacaacatg gtagcggcgc tttggggcac agccatcggc ggcggattca acattacaca
     1621 gcagtggttg cccatcaaaa aatatggccc ggccctgcct ttggtcatgc tgatttcggt
     1681 gtttacgggc ttgttttttc tggtgcggat gctgagttcc cattggcggc gttaccgcca
     1741 gatttacgcg gtggagcagt tcaaacgata ccacaccgac gagcaatgga tagccgtggc
     1801 cgaagaggtg ttcaacggtc cgcacgaccc gcacttcatc gaactgaggc ggcagtgcat
     1861 tcgctatggc tttggtttgg tggtcattgc ggccaacgaa gctccccgcg ctcacctcac
     1921 gcccgcccgc gaagacgtgt ttgaaggtcg gcgcgacatc gtcaaactca tcacagacaa
     1981 tgagttggtc aaaaacatgc aaaatctggg ccgccaagcc tggatcaccg aactctgggg
     2041 caaaatgtcg gcctacattc cccaaagcga tcaatggctg caccgtttcg gcggacggct
     2101 gcccaaacac tggctcgcct cggcggtggc ctttgtcttc atcatcgcaa tatggtatca
     2161 cgattacgac acctgggcgc accgcaaagt gacccccaaa gtgtatgccg aggaggccat
     2221 tcaggcgctg cgggcactgc ccccggagcc gtcaaccatc aatctcgata caccttattt
     2281 ttggcccgca ccctttctgg acgaccgcat ttcctacctc gatgaatggc tggaagaccg
     2341 gctgatcgct cagcgtttgt ttcgcaattc ggcccgcaac gctgccgccc gggaagacgg
     2401 gtacggcgtc attttttacg attgcgcgcg ttttctcaat ttcgagggtg tcaaattttt
     2461 acttttggac ggcgtgtatt ccgattttga atcctcgctc ttgcgggtag aaatgctcca
     2521 ggccgacggc attcaggcgg gcagtttgtg gctgggctgt tttgacgaac gcagcaaaga
     2581 atacgccgtg tatctggggc cgctgttcaa tacgcccggt gaagccggcc gcttggcccg
     2641 cgagttggaa tacctgcgca gcgaccgcag cagcagggat tttctcaccg tcaaagccat
     2701 caaagcagat gcctcc
//
LOCUS       DAORLF010000555         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20409_length_2711_cov_1.399096, whole genome shotgun sequence.
ACCESSION   DAORLF010000555 DAORLF010000000
VERSION     DAORLF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20409_length_2711_cov_1.399096"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /locus_tag="PK198_25835"
     CDS             <1..>321
                     /locus_tag="PK198_25835"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42248.1"
                     /translation="SEAASPVYYQVRLKKYDIEAELTATPRTGLHRYTFPAGAEAGLL
                     IDLVHRDEVLDAALWRRSDTEVEGYRISRAWAEEQHVYFSAKFSQPIAGLIFDGKAKS
                     DTLRG"
     assembly_gap    322..472
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <473..>1802
                     /locus_tag="PK198_25840"
     CDS             <473..>1802
                     /locus_tag="PK198_25840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRF42249.1"
                     /translation="VWNQQLEKIRVESHNDEEARIFYTALYHASLQPNLFTDADGRYR
                     GHDRQIHQAKEHTQYTVFSLWDTYRAANPLYHIIEPERSRDFVHTFLQQFRQSSELPV
                     WELAGNETYCMIGYHSAAVIADAWAKGIRDFDPDLALEAMIATANQDKFGKKFYREYG
                     FVPAEMEAESVSKTLEYAYDDWCIAQMAQALGRQEMYETFIRRAQSYQNLFDPSTGFF
                     RARRNQSWIAPFDPAEVNYHFTEANAWQYGFYAPQDVEGWMALLGGDEKAAQHLNAVF
                     TADSKTTGRDQADITGLIGQYAHGNEPSHHIAYLYNFCGQPWKTQQRVQEIMRSQYAD
                     APEGLSGNEDCGQMSAWYIFSALGFYPLTPGSTAYVIGSPRVKNAEIKVGKGKTFQIT
                     VKNGGPQSPYIQSVQLNGKAYRKSFIRHEDLLAGGTLVFTMSEQPNKSWGT"
     assembly_gap    1803..1812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1813..>2063
                     /locus_tag="PK198_25845"
     CDS             <1813..>2063
                     /locus_tag="PK198_25845"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HRF42250.1"
                     /translation="PEDRPRSHIENNRLMPLPVVSRGQRAFFGTDTIKLAHPLPDAEI
                     WFSTDGSDPLSNGKKYAQPIVIKQDTEIKAIAKHPKWGQ"
     assembly_gap    2064..2160
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2161..2569
                     /locus_tag="PK198_25850"
     CDS             <2161..2569
                     /locus_tag="PK198_25850"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRF42251.1"
                     /translation="PSALIDMLEGGNDYRTGEWQGFEGVDVDAVVDLGAAQQLRQVRI
                     HFLQDENAWIFMPTGVSFYGSTDGRNFRLIGKVPARTRPEHKGTIIESFSVKTSGPMR
                     YIRVVGHNRGLCPPTHKGAGGKAWIFADEIRVE"
BASE COUNT          599 a          764 c          637 g          453 t
ORIGIN      
        1 tccgaagcgg cttcgcccgt ttactatcag gtgcgcctca aaaaatacga catcgaagcc
       61 gagctgacgg ccactccgcg cacgggcctg caccgatata ccttccccgc aggcgctgag
      121 gccggcctgc tcattgatct cgttcaccgc gacgaagtat tagacgccgc cctctggcgc
      181 cgcagcgata ccgaggtgga gggctatcgc atctcgcggg cctgggctga ggagcagcac
      241 gtttattttt cggccaaatt ttctcaaccc atcgccgggc ttatcttcga cggcaaggcc
      301 aaatcagaca ccctgcgcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtctgga
      481 accagcaatt ggaaaaaatt cgcgtggaat ctcacaacga cgaggaggcc cgcatttttt
      541 acacggccct gtaccacgcc tcgctgcaac ccaatttgtt caccgatgcc gacgggcgtt
      601 atcgcggcca cgaccgccaa attcatcagg ccaaagagca cacgcaatac accgtttttt
      661 cgctgtggga tacctaccgc gccgccaatc cgctgtacca catcattgag ccggagcgca
      721 gccgcgattt tgtacacacg tttctgcaac agtttcgcca aagcagcgag ctgcccgttt
      781 gggaattggc cggcaacgaa acctactgca tgatcggcta ccactcggct gccgtcatcg
      841 ccgacgcctg ggccaaaggc atccgcgatt tcgaccctga tctggctttg gaagccatga
      901 tagcaacggc caatcaggat aagttcggca aaaagttcta ccgcgaatac ggcttcgtac
      961 ccgccgaaat ggaggccgaa tctgtttcca aaacgctgga atacgcctac gacgactggt
     1021 gcatcgccca aatggcgcag gcgttgggcc gacaggagat gtatgaaacc ttcattcgcc
     1081 gggcgcaatc gtaccaaaac cttttcgatc cttccaccgg ctttttccgc gcccgccgca
     1141 accagagttg gattgcgccc ttcgaccccg ccgaggtcaa ttaccacttc accgaggcca
     1201 atgcctggca gtatggattt tatgcgccgc aggatgtaga aggctggatg gcgctgctgg
     1261 gcggagatga aaaagccgcg caacacctga acgccgtgtt caccgccgac tccaaaacca
     1321 ccggccgcga ccaggccgac atcaccggcc tcatcggcca atacgcgcac ggcaacgaac
     1381 ccagccacca catcgcctac ctctacaatt tctgcgggca gccctggaaa acgcagcagc
     1441 gcgtgcagga aatcatgcgc agccaatacg ccgacgcgcc ggagggcctg tcgggcaatg
     1501 aagactgtgg ccaaatgtcg gcctggtaca ttttcagcgc gctcggattt tacccgctca
     1561 cgcccggcag cacggcctat gtcatcggtt cgccgagggt gaaaaatgcc gaaatcaagg
     1621 taggaaaagg caagactttc cagatcaccg tcaaaaacgg cggcccgcaa tcgccttaca
     1681 ttcagtcggt tcagctcaac ggcaaggctt atcgaaagtc cttcatccgc cacgaagacc
     1741 tcctcgccgg cggcacgctg gtatttacca tgagcgagca gcccaacaaa tcctggggca
     1801 cannnnnnnn nncgcccgaa gatcgccccc gcagccacat cgaaaacaac cgcctcatgc
     1861 cgctgccggt cgtgtctcgc ggccaacgcg ccttcttcgg aaccgacacc atcaaactgg
     1921 cgcaccccct gcccgatgcc gaaatctggt tcagcaccga cggcagcgac ccgctttcca
     1981 atgggaagaa atacgcacaa cccattgtca ttaaacaaga tacggagata aaagccatag
     2041 cgaagcatcc caaatggggc caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 tcccagcgcg ctcattgaca tgctcgaagg cggcaacgac taccgcaccg gcgagtggca
     2221 gggcttcgag ggcgtggacg tggacgccgt ggtggacctc ggcgcagcgc agcaactcag
     2281 gcaagtacgc atccactttt tgcaagacga aaacgcctgg atttttatgc ccaccggcgt
     2341 ctcgttttac ggcagcaccg acgggcgcaa tttccggctc atcggcaagg taccggcccg
     2401 cacccgcccc gaacacaagg gcaccatcat cgaatcgttc agcgtaaaaa cttccggccc
     2461 gatgcgctac atccgtgtgg tgggccacaa tcgcggactt tgtccgccca cacacaaagg
     2521 cgccggcggc aaagcctgga tttttgcaga tgagatcagg gtggagtgat ggtttttata
     2581 aatattttga aaaatttaat aactcgataa aatggaaaag agattagtaa aaatcgcgaa
     2641 agagcttaat gtaagtataa ccacgattgt ggaattcctg cataagaacg gctatgaaat
     2701 tgaaaacaag c
//
LOCUS       DAORLF010000556         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20511_length_2700_cov_1.471834, whole genome shotgun sequence.
ACCESSION   DAORLF010000556 DAORLF010000000
VERSION     DAORLF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20511_length_2700_cov_1.471834"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK198_25855"
     CDS             complement(<1..695)
                     /locus_tag="PK198_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRF42252.1"
                     /translation="MLKIAFAPIYRYELPPGHRFPMEKYEILPEQLLYEGTVRPDNFF
                     EPQPIDEPTILLTHEAEYWQKLKCQTLNVKEIRAIGFPMTPRLVERGRYIAAGTIGCA
                     LYARQHGVSLNIAGGTHHSFSYKGEGFCVFNDIAIAANYLLHHGLSRQILVVDLDVHQ
                     GNGTAQIFENEPKVFTFSMHGEKNYPVRKERSDLDIGLPDGTEDAAYLRILRDTLPRL
                     LDSVQPDFVFYQSG"
     gene            complement(711..833)
                     /locus_tag="PK198_25860"
     CDS             complement(711..833)
                     /locus_tag="PK198_25860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42253.1"
                     /translation="MRLSLADNLMVGIVSGIPIARENQRFSSFVRIIYEPGHRH"
     gene            945..>1900
                     /locus_tag="PK198_25865"
     CDS             945..>1900
                     /locus_tag="PK198_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRF42254.1"
                     /translation="MLPPTITKNYIVGLIAIAGLLWACTGGGRKEAHLIEYWSSNNGG
                     EIQFSKWAVERWKQQHPGKPVKYQPVPEGQSSEEIIMAAVVAGTTPDIYSNLWQGLVE
                     FYSRSGVLVALDTLNGFMEYIHERCDSATVAEITSSDGHIYQVPWKINPIMTIYNKQL
                     LRQTGLDEMPAAYSDYLRAARSFRKDTDSDGYVDQWFGNTSVKLAWYQRLFNFYALYL
                     AASGGMPLVKDGRANFNNEHAVEVFRFLQELYRENYFSKQVESAGQDLFIAGRFASKW
                     TGPWEIEYLEKFKRPGFEYDFAPMPVPDGHQGPVFTYCDPKN"
     assembly_gap    1901..1913
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1914..2264
                     /locus_tag="PK198_25870"
     CDS             <1914..2264
                     /locus_tag="PK198_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF42255.1"
                     /translation="VFNTCKNPQAAFDFIRSMTDPEADLHFLRTTRQLPRRKDLDNWP
                     GFKDFFAGNPQMSAFARQARYVRGADNSEALIEVFDIISQEYEACVLYQTKTPERAVA
                     DAERAVNVLLRSKE"
     assembly_gap    2287..2298
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2367..>2700
                     /locus_tag="PK198_25875"
     CDS             2367..>2700
                     /locus_tag="PK198_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRF42256.1"
                     /translation="MHLLLFVLFPIVFSVVLAFHKWNIISPMEYVGLDNIQRLFQDRL
                     FWKSVLNTLKFLIVHIPLQIVIALALAYFLNEKILMRGFFRATFFLPVVISGVVVTTI
                     GAGGGQNHP"
BASE COUNT          632 a          699 c          717 g          627 t
ORIGIN      
        1 cccgattggt aaaaaacaaa atccggttgt accgaatcca gcaggcgggg cagcgtatct
       61 ctcaaaatgc gcagataggc ggcgtcttcc gtgccgtcgg gcaggccgat gtcgaggtcg
      121 gagcgttctt tgcgcaccgg atagtttttt tcgccgtgca tgctgaaagt gaacactttg
      181 ggttcgtttt caaaaatctg cgcggtgccg ttgccctgat gcacgtctaa gtccactacc
      241 agaatctgcc gggacaatcc atgatgcagc aggtaattgg cggcaatggc aatgtcgttg
      301 aaaacgcaaa aaccctcgcc tttgtagcta aacgaatggt gcgttccacc ggcaatattg
      361 agcgacacgc catgctgccg ggcgtacagc gcacaaccga tggtaccggc tgcgatgtac
      421 ctgccccgct ctaccaagcg cggggtcatg ggaaagccga tggcgcgtat ttctttgacg
      481 ttcagggttt ggcatttcag tttttgccag tactccgctt cgtgcgtcag caggatggtc
      541 ggctcgtcta tgggttgcgg ctcaaagaag ttatcggggc gcaccgtgcc ttcgtacagc
      601 aattgctccg gcaaaatctc gtacttctcc atcgggaagc ggtggcccgg cggcagttcg
      661 tagcggtaga taggagcgaa ggcgattttg agcataaaaa aatgggggcc tcagtgccga
      721 tgccccggtt cgtaaataat gcgcacaaag ctactgaatc gctggttttc gcgggctatg
      781 ggaatgccgg aaacaatacc gaccatgagg ttgtcggcca gactgaggcg catttggggt
      841 cgaatggtca tatcgaagtc atgaaaaaat atgtaaatct tgcatcataa taccagaatt
      901 gccggagctt agtgtcgaaa tttgccttgc caacctgtat tccaatgctg ccgccaacca
      961 tcacaaaaaa ttacatcgtc gggctgatcg ccatagcggg tcttctgtgg gcctgcacgg
     1021 gaggcgggcg gaaagaggcg cacctcatcg aatactggtc gtcgaacaac ggcggcgaaa
     1081 tacaattttc aaaatgggcc gtggaacgct ggaaacaaca acaccccggc aagcccgtta
     1141 aataccaacc cgtaccggaa gggcaatcga gcgaagaaat catcatggcg gcggtagtgg
     1201 ccggcaccac gcccgatatt tattccaacc tctggcaggg cttggtggag ttttacagcc
     1261 ggtcgggggt attagtggcc ttagatactt tgaacggctt catggagtac atccatgagc
     1321 gctgcgactc ggccaccgtg gcggaaatca cctcctccga cggccatatt tatcaggtgc
     1381 cctggaagat caatcccatt atgaccattt acaacaagca actgctgcgg caaacgggct
     1441 tagacgaaat gccggcggcc tattccgact atctccgggc agcccgaagt ttcaggaaag
     1501 acaccgactc ggacggctat gtggatcagt ggtttggaaa tacttccgtc aaattggcct
     1561 ggtaccagcg gctcttcaat ttttatgcgc tgtatctggc cgcttccggc ggaatgcctt
     1621 tggtgaaaga cggtagggcc aatttcaaca acgaacatgc cgtggaggtg tttcgctttt
     1681 tgcaggaact gtaccgggag aactattttt ccaaacaggt agaatcagcc gggcaggatt
     1741 tgttcatcgc cgggcgtttt gcgtccaaat ggacaggccc ctgggaaata gagtatctgg
     1801 aaaaattcaa acgacccggt tttgaatacg attttgcgcc catgcccgta cccgacggcc
     1861 atcaaggacc agtctttacc tactgcgatc ccaaaaacat nnnnnnnnnn nnngttttca
     1921 acacctgcaa aaatccacag gcggccttcg acttcattcg cagcatgacc gacccggaag
     1981 ccgatctgca ttttttaaga accacccggc aattgccccg gcgcaaagac ctcgacaact
     2041 ggcccggttt caaagacttt tttgccggca accctcagat gtcggccttt gcccggcagg
     2101 cgcgttatgt gaggggcgcc gacaactcag aagcgctcat tgaggtgttt gatatcatct
     2161 cgcaggaata cgaggcttgc gtgctttatc agaccaaaac gcccgaacgc gccgtagccg
     2221 atgcggaaag agccgtgaat gtgctgctgc ggtctaaaga ataaatgcta ccgaccatga
     2281 aaatacnnnn nnnnnnnnta caggtaaata aaggcggcaa aaaggggcgc gggcttgcac
     2341 cgtacatcat cctgtctccg tatttattgc atttgctgct gtttgtgctg tttcccatcg
     2401 ttttttccgt ggtgctcgct tttcacaagt ggaacatcat ttcgccgatg gaatatgtgg
     2461 gtttggacaa catccagcgg ctgtttcagg accgcctttt ttggaaatcg gtgctcaata
     2521 ccctgaaatt cctgatcgta cacatccccc tgcaaatcgt catagcctta gcccttgcct
     2581 attttctgaa tgaaaaaatc ctgatgcgcg gcttttttcg ggccacgttt tttctgccgg
     2641 tcgtcatttc gggcgtggtc gtcaccacga tcggggccgg aggaggacag aatcacccca
//
LOCUS       DAORLF010000557         2699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20523_length_2699_cov_1.683056, whole genome shotgun sequence.
ACCESSION   DAORLF010000557 DAORLF010000000
VERSION     DAORLF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20523_length_2699_cov_1.683056"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK198_25880"
     CDS             <1..610
                     /locus_tag="PK198_25880"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF42257.1"
                     /translation="FGDQLNPVNGNGNGQSSSETPPDNFTQYDFDAAAGLLIFNSNFH
                     FGVALHHISSPDVGFLNTSSNLYGGIPLRFALHGGLEIPILTNNNADGEAFLSPNILF
                     VSQGDFAQLNGGVHAGFGRFFAGASYRHTFGNADAAILTGGFRYGILKIGYSYDWTLS
                     ALANAGTGGAHEVSFALNLSESREWKRQRNKPDLNNCFKLFR"
     gene            716..>1338
                     /locus_tag="PK198_25885"
     CDS             716..>1338
                     /locus_tag="PK198_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF42258.1"
                     /translation="MKKLFKSGAIVLAAVFLLASCRSKENSSTTGWNYNDTKWGGFEK
                     LQYEGQATGPNLVLVEGGTFTMGVTEEDVTRDWNNVPRRVTVTSFYMDETEVANIDYR
                     EYLYWLNRVFGENLPEVVLDALPDTLVWRDELSYNEPMVETYFRHPAYDDYPVVGVNW
                     NQSNEYCKWRTDRVNEMILIQQGILSPNTEQVEADNFNTEAYLVGQY"
     assembly_gap    1339..1348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1349..2221
                     /locus_tag="PK198_25890"
     CDS             <1349..2221
                     /locus_tag="PK198_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF42259.1"
                     /translation="VRKNLKDLQTGGERAVRFEDGILLPAYRLPTEAEWEYAALSLKG
                     NQTTEQDEMISDRKLYSWNGNTMRYQKRNKYQGMMLANYKRSGGDYMGMAGKLNDNAC
                     PTAPVRSYLPNDYGLYNMSGNVNEWTADLYRPLTSMALSDAQNHDLNPYRGNVFMEKV
                     VDENGMPVEKDSLGRLRYQQVPDDKAVNRDNYKTGAAFNYLDGDQASQAFYEYGTTTL
                     ISDKARVIKGGSWADRAYWLSPGTRRYKEEDKADRTIGFRCAMTRTGSPTGNDAPGGH
                     HFKTRNKPVKRSFK"
     assembly_gap    2396..2425
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2426..>2699
                     /locus_tag="PK198_25895"
     CDS             <2426..>2699
                     /locus_tag="PK198_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF42260.1"
                     /translation="FDGNQFASAALQQGAAYAIVDDPGLAGLPRHILVPNVLTTLQDL
                     ARHHRRQFDIPFIGITGSNGKTTTKELVAGVLAMRYRVHFTQGNLNN"
BASE COUNT          681 a          741 c          648 g          589 t
ORIGIN      
        1 atttggagac caactcaacc ccgtcaacgg aaacggcaac ggacaaagct cctcggaaac
       61 gccgcccgat aattttactc aatacgattt cgatgcggct gcgggtctgc ttattttcaa
      121 ttccaacttt cactttggcg tggcgctgca ccacatcagc tctcccgacg taggctttct
      181 caataccagt tccaatcttt atggaggcat tccactgcgc ttcgccctgc atggcggcct
      241 cgaaattcca atcttaacga acaataacgc agatggggag gcgttcctat caccgaacat
      301 tttgttcgta tcgcaaggag atttcgcaca actcaacgga ggcgtgcatg caggtttcgg
      361 acgttttttt gcaggcgcct cttacaggca cactttcggc aatgcagatg cagctatttt
      421 gacgggcggt tttcggtacg gtattctgaa aatcggatac agctacgact ggacactctc
      481 cgcattggcc aatgcaggta ccggcggcgc tcacgaagtg tcgtttgcac tcaatctgag
      541 cgagagccgg gaatggaaac gccaacgcaa taaaccggat ttgaacaact gtttcaagct
      601 cttccgttga gccggaaaaa atacaaatgt taaagccggc gtcctttctt gtgtggtaca
      661 acaggatttt ctctatattt gtcggtcgtt ttccaaaaac aaactttcta aaggaatgaa
      721 aaaactgttt aagtctggcg ccattgtact ggccgccgtg tttttgctgg cgtcctgccg
      781 cagcaaagag aactcctcca ctaccggatg gaattacaac gacaccaaat ggggcggctt
      841 tgaaaagctg caatatgaag gccaggcaac cggcccgaac ctcgttttgg tggaaggcgg
      901 tacattcacc atgggtgtta ccgaagaaga tgtgacccgc gactggaaca acgtgccgcg
      961 ccgcgtcacg gtgacttcgt tctacatgga cgaaaccgaa gtggccaaca ttgactaccg
     1021 cgaatacctg tactggctca accgtgtgtt tggtgaaaac ctgccggaag tagtgctgga
     1081 cgcgctcccc gacacgctgg tctggcgtga tgagctgtcc tacaacgagc ctatggtaga
     1141 aacttatttc cgccacccgg cttatgacga ctatcctgtg gtaggcgtca actggaacca
     1201 gtccaatgag tattgcaaat ggcgcaccga ccgcgttaac gagatgatcc ttatccagca
     1261 aggtattctt agcccgaata ccgaacaggt agaagccgac aacttcaaca cggaagccta
     1321 tctggtaggt cagtatcann nnnnnnnngt tcgcaaaaac cttaaagacc ttcaaaccgg
     1381 cggcgagcgc gctgttcgct tcgaagacgg catcctcctg cctgcttatc gcctgccgac
     1441 cgaagccgag tgggaatacg cagccttgtc gctgaaaggc aatcaaacta ccgaacagga
     1501 tgaaatgatc tccgaccgca agctctattc ctggaacggc aacaccatgc gctaccaaaa
     1561 acgcaacaag taccagggca tgatgttggc caactacaag cgcagcggcg gtgactatat
     1621 gggtatggcc ggcaagctca acgacaacgc ttgtcctacg gcgcctgtgc gttcttatct
     1681 gcctaatgat tatggcctgt acaacatgtc gggcaatgtc aacgaatgga ctgccgactt
     1741 gtatcgcccg ctcaccagca tggcgctcag cgatgctcaa aaccatgatc tcaaccctta
     1801 ccgcggtaat gtattcatgg aaaaagtagt ggacgaaaat ggtatgccgg ttgaaaaaga
     1861 cagcttgggc cgcctccgct atcagcaggt tcccgatgat aaagccgtca accgcgacaa
     1921 ctataaaaca ggcgctgctt tcaactatct tgacggcgac caggcctctc aggcattcta
     1981 cgagtatggc accaccactt tgatcagcga caaagcacgc gttatcaaag gcggcagttg
     2041 ggccgaccgc gcttactggc tctctcccgg cacccgccgc tacaaagagg aagacaaagc
     2101 agatcgcacc attggtttcc gttgtgccat gactcgcacg ggtagcccca ccggcaacga
     2161 tgctccgggc ggccaccact tcaagacgcg caacaaaccg gtgaagcgct cctttaaata
     2221 aggacaacca agaaataaaa tcctgacggg tttataaaac catctgagcc ggttcccttt
     2281 cgagtgagcc ggctcaaatt ttttatgcta tgcacatctc cgatttatac gctgtttttc
     2341 aagcgcatcc agaggtaacc cccgattcgc gccgcatcac agaagactgt ctgttnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnngttcg acggcaacca atttgcgtcg gccgcacttc
     2461 aacaaggcgc agcttatgcc attgtggacg accccggtct tgccggtttg ccccgccaca
     2521 ttctggtgcc caatgtacta acaaccttgc aagacctggc tcgccatcac agacggcaat
     2581 tcgacattcc tttcatagga atcaccggca gcaacgggaa aacgaccacc aaagaattgg
     2641 tagccggcgt attggccatg cgctaccggg tgcatttcac acaaggcaat ttgaacaat
//
LOCUS       DAORLF010000558         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20629_length_2689_cov_1.894837, whole genome shotgun sequence.
ACCESSION   DAORLF010000558 DAORLF010000000
VERSION     DAORLF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20629_length_2689_cov_1.894837"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /gene="rpsK"
                     /locus_tag="PK198_25900"
     CDS             complement(<1..273)
                     /gene="rpsK"
                     /locus_tag="PK198_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRF42261.1"
                     /translation="MAKATKKVKKRKVKVEPEGMAFVQASFNNIIVSIANKSGQVISW
                     ASAGKAGFRGSKKNTPYAAQVAASDAAKTAHEAGLRTAEVFVKGPGS"
     gene            complement(316..693)
                     /gene="rpsM"
                     /locus_tag="PK198_25905"
     CDS             complement(316..693)
                     /gene="rpsM"
                     /locus_tag="PK198_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRF42262.1"
                     /translation="MARIAGIDLPRNKRGVIGLTYIFGIGRTSAQEILGKAGIDESVK
                     VHEWTDDNVREISRIITTEYKVEGELRSEVQLSIKRLMDIACYRGLRHRKGLPLRGQR
                     TRTNARTRKGKRKTVANKKKATK"
     gene            complement(722..838)
                     /gene="rpmJ"
                     /locus_tag="PK198_25910"
     CDS             complement(722..838)
                     /gene="rpmJ"
                     /locus_tag="PK198_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRF42263.1"
                     /translation="MKVKPSVKKRTADCKIVRRKGRVYIINKKNPKYKQRQG"
     gene            complement(850..1068)
                     /gene="infA"
                     /locus_tag="PK198_25915"
     CDS             complement(850..1068)
                     /gene="infA"
                     /locus_tag="PK198_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRF42264.1"
                     /translation="MAKKNLIKQDGVIEEALSNAMFRVRLENDHQIIATISGKMRMNY
                     IRILPGDKVSVEMSPYDLSRGRIIYRYK"
     gene            complement(1163..1984)
                     /gene="map"
                     /locus_tag="PK198_25920"
     CDS             complement(1163..1984)
                     /gene="map"
                     /locus_tag="PK198_25920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRF42265.1"
                     /translation="MIYYKTEEEVELIRQNCLLVCKVLAHVGSMLRPGISGSVLDKAA
                     EDLIRDHGAVPGFKGYRGFPATLCVSANEHVVHGIPTDKQIFKEGDIVSVDCGVQKNG
                     YFGDAAYTFAIGDVPEDVMQLLRVTNRSLYKGIEQAVVGRRLGDVGFAIQEFVERDHG
                     YGVVRELVGHGLGRNLHEEPEVPNYGKRGKGPMLKDGLVIAIEPMVNMGKKEVVTSKD
                     GWTVFSKDRKPSAHFEHTVVVRKNKADILSDHSMIEEAIRGNAHIKGLEYRSAVA"
     gene            complement(2050..>2689)
                     /locus_tag="PK198_25925"
     CDS             complement(2050..>2689)
                     /locus_tag="PK198_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HRF42266.1"
                     /translation="KRMVGRGANAMPQAGARDFIPLKVNAAGVMPIIFAQALMFLPAT
                     VVQFASGGDQSAMGTNTFLRLLNDFTSAPYNIIYFILVVVFTYVYTALIVNPQQYAEY
                     LKRQNAFIPGIKPGKPTSDFIDAITTRITLPGSIFLAFIAILPAFAAAAGINQAFALF
                     FGGTSMLILVGVVLDTLQQIESHLLMRRYDGLVKSGRIQGRGSRMMGLGAGG"
BASE COUNT          727 a          691 c          536 g          735 t
ORIGIN      
        1 ggaaccgggg cctttcacaa atacctcggc ggtacgcaaa ccagcctcat gtgctgtttt
       61 agcggcatcc gaagcagcaa cctgagcggc ataaggcgta tttttcttag accctctgaa
      121 accagccttg ccggcagaag cccaagatat tacctgacca ctcttgttgg cgatagacac
      181 gatgatgttg ttaaagctcg cctgtacgaa agccatgcct tccggttcta ctttaacttt
      241 tctctttttt acttttttcg tcgccttagc catcgcttcg acctaagttt tagaaaatga
      301 aggaatgcaa accgattact tggttgcttt tttcttgttt gccacagttt tgcgcttgcc
      361 cttacgggtg cgtgcattgg tacgggtgcg ttgtccgcgc aatggaagcc ccttgcgatg
      421 gcgcaacccg cgataacagg caatatccat caaacgcttg atgctgagtt gtacttcgct
      481 gcgcaactcg ccctctacct tgtattcagt agtgataatg cgggaaatct cgcgaacatt
      541 atcatctgtc cattcatgga cttttacgct ctcatcaata cccgctttac cgagtatctc
      601 ttgggcagaa gtgcgaccga taccgaaaat ataggtcaaa ccaattacgc cccgcttatt
      661 tctgggcaaa tcaatacctg caatacgagc catatctgaa ttgtggtttc tctgttaaaa
      721 attacccctg acgttgcttg tacttagggt ttttcttgtt gatgatgtac acccgtcctt
      781 tcctccgcac aatcttgcag tcggccgtac gctttttcac cgatggtttc actttcatga
      841 caacacgatt tatttgtacc tgtatataat tcggccccga gacaaatcat agggcgacat
      901 ttcaaccgaa accttatctc ctggcaatat gcgaatataa ttcatgcgca ttttaccgga
      961 gatggtagca ataatctgat ggtcattctc taagcgaact cggaacattg catttgacaa
     1021 tgcttcctca atgactccat cttgcttgat caggttcttt ttggccatac ttcaattttc
     1081 ggagcgcaaa gataataagg ttttccgagt tggcaatttt tttgcaaaaa acctgattat
     1141 attttgcgca gactttcatc cattaagcta ctgctgatct gtattccagt ccctttatat
     1201 gagcattgcc acgaatggct tcctctatca tggaatgatc cgaaagaata tccgctttgt
     1261 ttttgcgaac caccactgta tgctcaaaat gagcagaggg cttcctgtcc ttagaaaaaa
     1321 cagtccaacc atctttcgac gtaactactt cttttttgcc catattaacc attggctcaa
     1381 tagcaataac caagccatct ttcaacatag ggcctttacc ccgcttaccg tagttaggca
     1441 cctccggctc ttcatgtaaa ttacgcccta aaccgtgccc cactaattcg cgcactacac
     1501 catatccgtg atctcgctca acaaactctt gtatagcaaa acccacatca cctaatcggc
     1561 gtccaaccac agcctgttca atacctttgt ataaagacct gttggtaact cgaagtaatt
     1621 gcattacatc ttcgggtaca tctccaatag caaaagtata tgcagcgtct ccaaaatatc
     1681 catttttttg aaccccgcaa tcaaccgaca cgatgtcgcc ctctttgaag atttgcttat
     1741 ccgtaggaat accgtgtact acatgttcgt tagcggaaac acaaagcgtt gcaggaaacc
     1801 cccggtatcc tttgaagccg ggtacggcgc cgtggtctct tatcaggtcc tcagctgctt
     1861 tatccaacac ggaacctgaa atacccgggc gtaacataga acctacatgg gccagaactt
     1921 tacaaaccaa aagacagttt tggcgaatca gctcaacttc ttcttctgtc ttgtaataaa
     1981 tcatagcagc ttggcctatt aaaaatggtg actaaaaaaa tcaggtttta caggacggat
     2041 ggttaaaaat caacctcctg cacccagccc catcattcgg ctgccacgcc cttgaatacg
     2101 accggattta accaagccat cgtaacggcg catcaacaaa tggctttcta tttgctgaag
     2161 cgtatccaaa acaactccga caagaatcag catagaagta cctccaaaga aaagtgcaaa
     2221 ggcctgattg atacctgcag cagcagcgaa agccggaaga atggcaatga aagccaagaa
     2281 aatagacccc ggaagtgtga ttctcgttgt gatcgcatca atgaagtctg aagtcggctt
     2341 accaggcttt atgccaggaa taaatgcgtt ctgacgcttg aggtactcgg catactgctg
     2401 agggttcaca atcagtgccg tatatacata tgtgaacacg accaccagaa tgaaataaat
     2461 aatgttatac ggagccgagg tgaaatcgtt caacaacctc aaaaatgtat tcgtacccat
     2521 agccgactgg tctccgccag acgcgaactg aacgaccgtt gccggaagaa acatcagtgc
     2581 ttgtgcaaag atgatgggca ttacaccggc tgcattgact ttcaatggaa tgaaatcacg
     2641 cgcaccggct tgaggcattg cattggcgcc acgccctacc attcgctta
//
LOCUS       DAORLF010000559         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20684_length_2683_cov_2.042618, whole genome shotgun sequence.
ACCESSION   DAORLF010000559 DAORLF010000000
VERSION     DAORLF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20684_length_2683_cov_2.042618"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK198_25930"
     CDS             <1..468
                     /locus_tag="PK198_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42267.1"
                     /translation="VIGLSFTNCKRAAEKTAETTAEAIIESATDSDVDIKQDGKKISI
                     KTDEGDVNINFGGDADWPADMPKDVPKWTLGKIKGNTTAKAPEADTWTVYLENVNIGQ
                     IEAYEAELKKAGFKTAIFKMDDGGSVSGEKGKLQVACFMAGSDAALTASVRKN"
     gene            complement(469..1215)
                     /locus_tag="PK198_25935"
     CDS             complement(469..1215)
                     /locus_tag="PK198_25935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF42268.1"
                     /translation="MDSENTAAWYEDWFDSPYYSILYQNRDETEARQFIDALLANLQP
                     PPSARVLDLACGKGRFSKYLAEKGFDVTGLDLSFNSIQYARQFESDSLAFYTHDMRLP
                     YRMRYYNYIFSFFTSFGYFDTDRDHIRTLKSIRAGLRAGGIFVLDFFNARYVRKGLPM
                     QEEKELEGIRFQISKTEDGRFVTKSIQFEDAGRAYRFEERVRLYEQSDLERLFHAAGL
                     EITDVYGDYTLQSFDLQSSPRIILVARAIV"
     gene            complement(1280..1552)
                     /locus_tag="PK198_25940"
     CDS             complement(1280..1552)
                     /locus_tag="PK198_25940"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF42269.1"
                     /translation="MKNTISIKLLGMGDARYRRIKSNLTEALQHTPVAARMEEVMDLE
                     SILGYNVSSVPAIIVEDKILFEYGDIPSAEQLESMLRAAFSEEAHS"
     gene            complement(1666..2406)
                     /gene="fabG"
                     /locus_tag="PK198_25945"
     CDS             complement(1666..2406)
                     /gene="fabG"
                     /locus_tag="PK198_25945"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRF42270.1"
                     /translation="MLQGKIALVTGGSRGIGAAIALRFAQQGADVAFTYHSSEEKARA
                     VEAELAALGVRAKAYRSDASSYQEAEALINAVLEDFGQLDVVVNNAGITQDTLMLRMT
                     EEQWDCVIETNLKSVFNLTKHALRPMMKNRGGSIINMSSVVGVTGNAGQANYAASKAG
                     IFGFTKSIAKEMGSRNIRCNAVAPGFIETDMTHILDEKTKDAYLGAIPLKRLGRAEEV
                     ADLCVFLGSDMSTYISGQTISICGAMTT"
BASE COUNT          681 a          671 c          692 g          639 t
ORIGIN      
        1 gtaataggtt tgtcttttac caactgcaaa agagcagccg aaaaaacggc cgaaaccact
       61 gccgaggcca ttattgaatc tgccaccgac tccgatgtgg acataaaaca ggatggaaaa
      121 aaaatcagca tcaaaacaga cgaaggcgac gtcaatatca attttggcgg cgatgccgat
      181 tggccggccg acatgcccaa agacgtgccg aaatggacgc tgggtaaaat aaaaggcaac
      241 acaaccgcca aagcgcccga agccgatacc tggacggtgt atttggaaaa cgtaaacatc
      301 gggcagatag aggcttatga agccgaactc aaaaaagcgg gattcaaaac cgccattttc
      361 aaaatggacg acgggggcag cgtttccggc gagaagggca aattgcaggt agcttgtttt
      421 atggccggtt ccgatgcggc cctcaccgca tccgtgcgca aaaactgatt atacaatcgc
      481 tctcgctacg agaatgatcc ttggactact ttgtaaatca aatgattgca atgtgtagtc
      541 gccataaaca tctgtaattt ccagcccggc tgcatgaaac agtcgttcca gatcagactg
      601 ctcgtacagg cgtacccgct cctcaaaacg gtatgcccgg cctgcgtctt caaactgtat
      661 gcttttcgtt acaaaccgac cgtcttccgt ttttgaaatc tgaaaccgga tgccttccaa
      721 ctccttctcc tcctgcatgg gcagcccctt gcgaacataa cgggcgttga aaaaatcgag
      781 tacaaaaatg ccacctgccc gcaagcctgc gcgaatgctc ttcaaggtgc ggatgtggtc
      841 gcggtcggta tcgaagtagc cgaaactggt aaaaaaactg aagatgtaat tgtagtagcg
      901 catccggtaa ggcagccgca tatcgtgggt atagaaggca aggctgtcgg attcaaactg
      961 ccgggcgtat tgaatgctgt tgaacgacaa atcaagtccg gttacatcaa agcccttctc
     1021 tgccaggtat ttggaaaaac gccctttgcc acaagccaga tcgagaacgc gggcggatgg
     1081 gggcggttgc aagttggcca acaatgcgtc tatgaattgg cgggcctcgg tttcatctct
     1141 gttttggtat aaaatggagt agtaaggcga gtcgaaccag tcttcgtacc aagctgctgt
     1201 attttcggaa tccataaggc tgtctgagtg aggcggcaaa agtatgttaa gttggccatg
     1261 tttacaaacg catccatcct taagagtggg cttcttccga gaaagcagct ctcaacatag
     1321 actccaactg ttctgccgaa ggtatatcac cgtattcaaa caggattttg tcttccacaa
     1381 tgatggcggg caccgaagac acattgtatc cgaggatgga ttccaaatcc attacctcct
     1441 ccatgcgggc ggctacgggg gtatgctgga gcgcctcggt caggttgctt ttgatgcgac
     1501 ggtagcgggc atcgcccata ccgagaagtt ttatagaaat agtatttttc acttgtttcc
     1561 ttgaatttcc taattggaag ttctttcaag gctcaagttt acaggcaatt tggttgccgg
     1621 cgaatgattt tcgtcagccg ccggcattag ttttgtcacc ctgtatcagg tagtcattgc
     1681 gccacaaata cttatggttt gtccggagat ataagtggac atgtcggaac cgagaaaaac
     1741 acacagatca gctacctcct cagcgcggcc cagccttttg agcggaatgg cgccgaggta
     1801 ggcgtctttg gttttttcgt ccaaaatgtg ggtcatatcg gtttcgatga agccgggcgc
     1861 cacagcattg cagcggatgt tgcgcgaacc catttctttg gcaatagact tggtaaatcc
     1921 gaaaatacct gctttggagg ccgcgtagtt ggcttgcccg gcattgcccg tcacacccac
     1981 caccgaactc atattaataa tggagccgcc cctgtttttc atcatcgggc gcagggcgtg
     2041 tttggtgagg ttgaataccg acttgaggtt ggtctcaatc acgcaatccc actgctcctc
     2101 ggtcattcgg agcatgagcg tatcttgcgt aataccggca ttattaacca ccacatccaa
     2161 ctgaccgaag tcttccagca ccgcattgat aagggcttcc gcctcctgat acgacgaagc
     2221 gtcggaacgg taggctttgg cgcgaacgcc caatgcggcc aattcggctt cgacagctct
     2281 ggctttttct tccgaagaat gataggtgaa ggccacatct gcgccctgct gcgcaaaacg
     2341 caatgcaatg gccgccccga tgccccgcga gccgcctgta actaatgcaa ttttaccttg
     2401 aagcaatttc atggtttttc ttttttgttt gaaagggcaa aggtaaggaa tatgtgcggt
     2461 aggcggtgct cggtcgcagt gctaagcgcg agggaacgcg aaatttttga acgcgaattt
     2521 gagcgaaggg tgcgaaattc cgcgaacgag agggaagctg cgacttgtac tctctacgta
     2581 gcttgcagct tgcaccgcgc cgtggcgcgg ttgtagccgt ttcaatccga aaccttgaac
     2641 ccgaaacccg ccttcgccaa ggcttcggcg gatgaaaccc gaa
//
LOCUS       DAORLF010000560         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20685_length_2683_cov_2.019026, whole genome shotgun sequence.
ACCESSION   DAORLF010000560 DAORLF010000000
VERSION     DAORLF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20685_length_2683_cov_2.019026"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK198_25950"
     CDS             complement(<1..555)
                     /locus_tag="PK198_25950"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRF42271.1"
                     /translation="MNRVIGRRTGWLLVFFLTMLSARAERFTPAVMDTGITCLMLNSV
                     AVVYEASGKIAISPDSVLRMPDSLFAAAASKAFLFGLTETPRWLRFQVVNPTADARRL
                     LLEIANPNLRHVEVYQYWGDLLIQSDTSGIDFPFERRPVPTHNFLFPMHLKAGDTLTC
                     LVYVAPSYQPANVDLYLWQRHHRVT"
     gene            774..2636
                     /locus_tag="PK198_25955"
     CDS             774..2636
                     /locus_tag="PK198_25955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42272.1"
                     /translation="MQATSRLLISIIFWLFAAHAAPAQTLPGFLHAELEQSDAPNILS
                     RMGHLHPTSSGYVTAGTWGDGEFSRAVFILTDRSFQPIQSYRINVQLPTPFGTTRWQE
                     SYGADVLPLGDNEYLILAAINMNPVMLLQPGSQNLDYAVLRVRVPANEQGAPQVLWAK
                     RFGGDFNDTPHNMERTSDGGFIISGYSNPEQALNTASIALTFLVKLDAQGNLQWARRY
                     RNNAADCRTTAFQLLTATLRRPVVQTQDGGFVFPLHCDENAYIVKVNTQGDVLWAKRF
                     EAGSGFFDGFFNQDWNFGLGIATGTGGAIIGVRELPNGNLVFLGNQFSFFTSIWGNPT
                     NGDGGGVVLPVSYVFTTTANGQFLHGNAFFRERFGDNNQPIEMIAHDFQPGPGNSLIV
                     ATGIRQNCFSGPECSFTPALLEINSAATGFDAAVTSAHEIISGSRHFSSHYPFALDFI
                     RIAPPDAEGRIALSYDKNMVLKSFAGASSCAPELAGLHSFPITLDLQNRSIIHTSLSG
                     ADLAVGTLSPTALQRSLPCQTSATQEPIAAKDSGIFIAPTVSNGHFNIQLSGGDEWMG
                     ARFHLLDLQGRPCLSGVIQSDRQAVAAGHLPAGVYLVRIVKDAKFTVLKLMLQP"
BASE COUNT          610 a          743 c          705 g          625 t
ORIGIN      
        1 cgtaacgcgg tgatgccttt gccagagata gaggtcgacg ttggccggtt ggtacgaagg
       61 cgccacataa actaagcagg tgagcgtatc gccggctttg aggtgcattg gaaaaaggaa
      121 gttatgtgtt ggtaccgggc gtcgttcaaa aggaaaatct atgccgctgg tgtcgctctg
      181 tatgagcaaa tcgccccaat attggtaaac ctcaacatgg cgaaggttgg ggttggcgat
      241 ctccagcagc aggcggcgcg cgtcggcagt cgggttcaca acctgaaagc ggagccatcg
      301 gggcgtttcc gtcaggccaa ataaaaaagc ctttgaggca gccgccgcaa acagggagtc
      361 gggcattcgg agtaccgaat cgggagaaat ggcaattttg cccgacgcct cataaactac
      421 tgctacggaa ttgagcatca ggcaggtaat gccggtgtcc attactgccg gagtgaatcg
      481 ctcggccctt gccgacagca tagtcaggaa gaaaaccaac aaccaaccag tccgtctgcc
      541 gatgaccctg ttcatggtaa actgtttttt ctacaacgcc ccactgtccc cgattgcaca
      601 gtactgaggc gcaaaatacc caaatccggt gagcggcata ccctttgatg gggagttttt
      661 agtaaaatga cgatggggta aaatacccat aagcggtgtt gcaggggctg gaaaataccc
      721 ggaactttgc cctgttatcc caaaatactt tttcaccatt aacaacaaaa ccgatgcaag
      781 ccacttctcg cctcctcatc tccatcattt tctggctgtt cgctgcccat gccgctccgg
      841 cacaaaccct gcctggtttt ctgcacgccg aattggaaca aagtgacgcc cccaatatcc
      901 tcagccgtat gggccatctc cacccaactt catcggggta tgtcaccgcc ggcacctggg
      961 gcgacggcga gttttcgcgc gctgttttca ttctgacaga caggtcgttt cagccgatcc
     1021 aatcctaccg catcaatgtg cagttgccca cgcccttcgg aactacccgc tggcaggaga
     1081 gctatggcgc tgatgtactg ccgctgggcg ataacgaata cctcattttg gcggccatca
     1141 acatgaatcc tgtgatgctg ctgcaacccg gctcgcaaaa tctggattat gcggtgttgc
     1201 gggtaagagt gcctgccaac gagcagggcg ccccgcaggt attgtgggcc aagcgcttcg
     1261 gcggagactt caacgatacg ccgcacaata tggaacgcac cagcgacggc ggcttcatca
     1321 tcagcgggta cagcaacccc gaacaggccc tcaacacggc cagcattgcc ctcacttttt
     1381 tggtaaaatt ggacgcgcag ggcaatctgc aatgggccag gcgctatcgc aataatgccg
     1441 ccgactgtcg caccacggcc tttcaactgc tgacggccac cctgcgccgc ccggtggtgc
     1501 aaacgcagga tggaggcttt gtttttcctc tgcattgcga tgaaaatgcc tacattgtga
     1561 aagtgaacac gcaaggcgat gtgctgtggg caaagcggtt tgaggccggc agtggatttt
     1621 ttgacggttt ttttaatcag gactggaact tcggacttgg cattgccaca ggaacaggcg
     1681 gcgccatcat cggcgtacgg gaattgccca acggaaatct ggtctttctc ggcaatcagt
     1741 tttctttttt caccagcatt tggggcaacc ccaccaatgg cgacggcggc ggcgtggtat
     1801 tgcctgtttc gtatgttttt accaccacgg ccaacgggca gtttttgcat ggaaacgcct
     1861 tcttccgcga acgattcggc gacaacaatc agcccatcga aatgattgcc cacgactttc
     1921 agcccggccc tggcaatagc ctcatcgtag cgactggtat tcgccaaaat tgtttcagcg
     1981 gccccgagtg cagtttcact ccggctttgt tggagatcaa ctccgctgct accggcttcg
     2041 atgctgccgt gacgagcgcc catgaaatca tttcgggaag tcgccatttt tcttcgcact
     2101 accccttcgc gctcgatttt attcgcatag cacctcccga tgccgaaggt cgcatcgctc
     2161 taagctacga caaaaacatg gtgctgaagt cgtttgccgg agcatcgtcc tgcgctcctg
     2221 aattggccgg cctgcattct ttccccatta ctttagacct ccaaaaccgc tccatcattc
     2281 atacctcgct atcgggagca gatttggccg taggaacgct ttcgcctacc gctttgcaac
     2341 gcagtctgcc atgccaaacc tctgccacgc aggaaccaat agcggccaaa gacagcggca
     2401 tttttatagc gccgacggtc tcaaacgggc atttcaatat tcagctttca ggcggagatg
     2461 aatggatggg tgctcgtttc caccttttgg atttgcaagg tcgtccttgc ctcagcggag
     2521 tcattcagtc agacaggcag gccgttgcgg cgggacatct gccggcaggc gtttatttag
     2581 ttcggatagt aaaagatgcg aagtttacgg tgctgaaatt gatgcttcag ccttagtagt
     2641 ggggcaaaaa taaatgctca ttttctttag gccaaaggat tag
//
LOCUS       DAORLF010000561         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20688_length_2683_cov_1.944064, whole genome shotgun sequence.
ACCESSION   DAORLF010000561 DAORLF010000000
VERSION     DAORLF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20688_length_2683_cov_1.944064"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>922)
                     /locus_tag="PK198_25960"
     CDS             complement(<1..>922)
                     /locus_tag="PK198_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42273.1"
                     /translation="AASGDEIWIAAGTYYPSKDETGSASPSDNRKKVFYINKDVAIYG
                     GFNGTETARSQRNWNTNIVTLSGDIGTIGSSTDNCYHVVKIYNVSNAMTLNGVQITGG
                     NASASGSTADGIGGGIIIQGTDGVSSSPLIDNCRIYANSASNGGGVGAFSLNNGSSIP
                     DFFNCIIDNNNATGVAGGVYNFSDGNFMGTPFSVATFEHCTFYENNSVSSTARVGYSF
                     AADDGGASLFIINSIAWGSATGVYFSKSTFGATHLQYSLSKGGCNGNTNCVGVILSSD
                     PLFVNAAGGNLRLNASSPAIDVAGTEYFNSP"
     assembly_gap    923..973
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <974..1172
                     /locus_tag="PK198_25965"
     CDS             <974..1172
                     /locus_tag="PK198_25965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42274.1"
                     /translation="LVPLLLSLPVGFTYFVAADDVAIQKQHSSNVSTKCADKLMFFSV
                     LILGDNNINTLMNEYLRDAFR"
     gene            complement(1190..1771)
                     /locus_tag="PK198_25970"
     CDS             complement(1190..1771)
                     /locus_tag="PK198_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HRF42275.1"
                     /translation="MKNTIIALSILAALAFTQCTNNAPAADTDATAAPTEETLTQRGE
                     YLVGILGCHDCHSPKKMGPQGPELIPELMLSGYPADRPFGKVNKEALAEGWALFNGDL
                     TAGAGPWGATFAANITSDASGIGEWSLEQFTRALTQGKAKGMEDGRPLLPPMPWFNYT
                     NMKPEDIEAIYTYLQSTKPVRNVVPAPIGPEAL"
     assembly_gap    1886..1911
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1912..>2683)
                     /locus_tag="PK198_25975"
     CDS             complement(<1912..>2683)
                     /locus_tag="PK198_25975"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HRF42276.1"
                     /translation="DAGANSIVKWEAATGDFSIVKTLDRFPNPLPFGPPMVDPVPTKA
                     LRKPDGTFYVSQLTGFPFLPGAANVHNLDAEGNLSVHAEGFTCLTDMHFDPTDGNLCV
                     MQFGVFGDVGGTLNFILGSAAVIKLMPDGSRDTIAQGIGGLAPSFAFDGDGNLYVADL
                     VFGQILKYNLLSSTQETPLAKTQLTVFPNPASEQVHIEYELTREAPVTMGIYDLGGRL
                     LASFNEGNMPSGKHRLTWNGTMAGGRVAQNGTYIYRLLA"
BASE COUNT          614 a          621 c          715 g          656 t
ORIGIN      
        1 agggctgtta aaatactctg tacccgccac atcaatagcg ggggaagaag cgttcaaacg
       61 caagttgccc ccggcggcat tgacaaacaa aggatctgag ctaagaataa cgccgacaca
      121 attcgtattg ccattgcatc ctcctttgct taaggagtac tgtaaatgcg tcgcgccaaa
      181 cgttgacttg gaaaaataga cgcctgttgc tgaaccccag gcaatagaat tgatgatgaa
      241 aagcgatgcg cccccatcat ccgctgcaaa agaatagcct actctagcgg ttgaactcac
      301 tgaattattc tcataaaagg tacaatgctc aaaagtggct acagaaaatg gggtacccat
      361 aaaattgcca tctgaaaaat tatatacacc ccctgctaca cctgttgcat tgttgttatc
      421 aataatgcaa ttgaaaaaat caggtatgct ggatccattg tttaaactaa aagcgccaac
      481 gccgccgcca ttgctggcag agttggcata tattctgcag ttatcgatta agggagatga
      541 tgatactccg tctgtgccct gtatgatgat acctccacct atgccatcag cagtcgaacc
      601 ggaggcactg gcattaccgc cagtaatttg tactccattc agggtcattg cgtttgaaac
      661 gttgtatatt tttacaacgt gatagcaatt atcggttgaa gacccaatcg tgcctatgtc
      721 gccgctcagg gtcacaatat tggtattcca attgcgctga ctacgcgcag tttccgtacc
      781 gttgaaaccg ccataaatag ccacatcttt gttgatgtaa aaaacctttt tgcggttatc
      841 agaaggtgat gcagatcctg tttcgtcttt tgaaggataa tacgtaccgg cggcaatcca
      901 gatttcatca ccggaagctg cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnngctggtg ccgttgttgc tgtcgttgcc ggtcggtttt acgtattttg
     1021 tggctgcaga tgatgttgca atacagaaac aacacagcag caatgtcagc acgaaatgtg
     1081 ctgacaaatt gatgtttttt agtgtactca tattaggtga taataatatt aacacgttga
     1141 tgaatgaata tttgcgggat gcctttcgtt gagacagtca ggtatttgct cacaaggcct
     1201 ccggccctat cggcgcgggc accacgttgc gcaccggctt ggtgctttgc aggtaggtgt
     1261 agatggcttc gatgtcctcc ggtttcatgt tggtgtaatt gaaccagggc atgggcggca
     1321 gcaaggggcg gccgtcttcc attcctttgg ctttgccctg cgtgagggcg cgggtaaact
     1381 gctccaatga ccattcgccg atgcccgatg cgtcggaggt gatgttggcg gcaaaagtgg
     1441 cgccccaggg gcctgcgccg gcagtgagat cgccgttgaa caaagcccag ccctcggcca
     1501 gcgcttcttt gtttactttg ccgaaaggcc ggtcggcggg atagccggaa agcatgagtt
     1561 cagggatgag ttcggggcct tgcggtccca tctttttggg cgagtggcag tcgtggcaac
     1621 cgagaatgcc gaccaagtat tcgccgcgtt gggtgagtgt ttcttcggtg ggcgcagccg
     1681 tagcgtctgt atcggcggcg ggtgcgttgt tggtacactg cgtgaaggcc agagcggcaa
     1741 ggatgctgag agcgatgatt gtgtttttca taatattttc ttttttaaga tgttgtgaaa
     1801 gaatgccggg acattttcta catgccccgg cccaaaacct cgatttgttt tatggtctct
     1861 gccaggaaat caaccctgcc gacagnnnnn nnnnnnnnnn nnnnnnnnnn ngccagaaga
     1921 cgatagatgt atgtgccgtt ttgtgccaca cgaccaccgg ccattgtgcc gttccaggtc
     1981 aggcggtgtt tgccggaagg catattgccc tcgttgaagg aagccagcaa tcggccgccg
     2041 agatcataaa tgcccatggt cacaggggct tcgcgggtga gttcgtattc aatgtgtacc
     2101 tgttcggaag cagggttggg aaacaccgtg agttgcgtct tggccaaagg cgtttcctgg
     2161 gtgctgctca gcagattgta tttcagtatc tggccgaaaa ccaagtctgc cacatacaaa
     2221 ttgccgtcgc cgtcaaaagc aaaactgggc gccaaaccgc cgatgccttg cgcgatggtg
     2281 tcgcggctgc cgtcgggcat aagtttaatg actgccgccg atcccaatat gaaattgagc
     2341 gtgcctccta catcgccaaa cacgccgaac tgcatcacgc agaggttgcc gtcggtagga
     2401 tcaaagtgca tgtcggtgag gcaggtgaag ccctcggcgt gtacgctgag attgccttcc
     2461 gcgtcaagat tgtgtacatt ggctgctcct ggcagaaaag gaaaaccagt gagttggctg
     2521 acgtaaaatg tgccgtcggg tttgcgcagt gctttggtag gtacggggtc caccatcggt
     2581 gggccaaagg gcagcggatt gggaaagcga tctaatgttt tgacgatgct gaaatcgccg
     2641 gtagcagcct cccattttac gatggaattg gcgcccgcgt cgg
//
LOCUS       DAORLF010000562         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20693_length_2683_cov_1.578767, whole genome shotgun sequence.
ACCESSION   DAORLF010000562 DAORLF010000000
VERSION     DAORLF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20693_length_2683_cov_1.578767"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1515)
                     /locus_tag="PK198_25980"
     CDS             complement(102..>1515)
                     /locus_tag="PK198_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HRF42277.1"
                     /translation="RWINAYAWQYVLLLFAGCFTLIANLDYIITFLRGRLKVGGSVLA
                     HIGFGMMIVGILASGLNKRFVSINTFVMDGLIEGGDSERLKNNVLLFKGVPMQMAGYE
                     VTYVSDTLDNVTRTYTVNYQKKDESGKVLEEFDLHPNILYDKSFTKIAASNPDTKQYL
                     HKDIFTHISSLPQVEMDMEFRKKREDSLRYQPHSLRLGQTYSFTDTVLLRDRDTFVLK
                     QYSLRLEEITREATHPDYKAQQGDLTAGAVISVKRDDDDSTYTVTPMVILRGQLVYYF
                     PSQINELSARVRLHPDVFDQIYTPEESLTYKEFTFKQGQIVDFEGLQLRFDGFNPKAE
                     HPALQKEESDLAVGARMSVLTPQGGQFQAQPVFFIRDNTPFNLKDEVENLGLHLRFLS
                     LDPKTETIQLMAARKLPQEDFPAAIELATNSLRSDYIVMEAIEFPGINLFWLGSTLMM
                     LGLGLSMTHRIGQLYKKERA"
     assembly_gap    1516..1579
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1580..>2683)
                     /gene="ccsA"
                     /locus_tag="PK198_25985"
     CDS             complement(<1580..>2683)
                     /gene="ccsA"
                     /locus_tag="PK198_25985"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF42278.1"
                     /translation="LEFKYIFASIWEGQEGSFLLWMFWHVVLGGLLVASAKNWESPVL
                     AVLMLIEVFISIMILGIYIGEVRIGSNPTLLLRNTMDAPIFANADYLSLIKGNGLNPL
                     LQNYWMTIHPPTLFLGFASTAVPFAFAIAGLWKKDFEGWIKPALPWALFSGMILGTGI
                     LMGGAWAYEALSFGGYWAWDPVENMSLVPWLTLLAGIHTHLVAKSTGYSIRSTFFFYI
                     ISFVLVLYSTYMTRSGVLGDTSAHAFTEMGLETQLVLFVLTFLLIGLVLLIARFRQIP
                     EPKPEESLVSKEFWLFIGSLVLLFSALLITASTSLPVFNKIMESFNPAYVGRVITDPI
                     PHYNKYQLWIGIFIGLLSGGVQFLRFREMNWAGN"
BASE COUNT          602 a          646 c          786 g          585 t
ORIGIN      
        1 gtttctgctg aacacctgca cgggcggcca accgcgttcg gccaatcggc gagccaaatg
       61 tgcgcccaca ttgccggctc cgacgagcac gacccgcgtg gtcatgcccg ctcttttttg
      121 tacagttggc cgatgcggtg cgtcatgctc aggccgaggc ccagcatcat gagcgtggaa
      181 ccgagccaga acaggttgat gccgggaaat tcaatggctt ccatgacgat gtagtcggag
      241 cgcaaagagt tggtagccaa ttcaatagcg gcaggaaagt cctcttgagg aagcttgcgg
      301 gccgccatga gttggatggt ttcggttttc gggtccaatg agaggaagcg caggtgcagc
      361 cccagatttt ccacttcgtc tttgaggttg aagggcgtgt tgtcgcggat gaagaagacg
      421 ggctgcgctt gaaattgccc gccctgtggc gtgaggacgc tcatgcgagc gcctacggcg
      481 aggtcgcttt cctctttttg gagcgccggg tgttcggctt tgggattgaa gccgtcgaag
      541 cgcaattgga ggccttcaaa atcaacgatt tggccttgtt tgaatgtgaa ctctttgtag
      601 gtcagcgatt cttcgggcgt ataaatctgg tcgaacacat cgggatgcag gcgcacgcgg
      661 gcgctcagtt cgttgatttg cgaagggaaa taatacacca actgcccgcg cagaatgacc
      721 atgggcgtga cggtgtaggt actgtcgtcg tcgtctcgtt tgacgctgat gacggcgccg
      781 gcggtgaggt cgccttgttg cgctttgtag tcggggtgcg tggcttcgcg agtgatttcc
      841 tctaaacgca gggagtactg tttgaggacg aaagtgtcgc gatcgcgcag gagcacggtg
      901 tcggtgaagg agtaggtttg gccgagtcga agcgagtgcg gctggtatct caggctgtct
      961 tcgcgttttt tgcgaaactc catgtccatt tccacctgcg gcagcgagga gatgtgggtg
     1021 aaaatatcct tgtgcaggta ctgtttggtg tcgggattgg aggcggcgat tttggtgaaa
     1081 gacttgtcgt acaggatgtt gggatgcagg tcgaattcct ccagcacctt gcccgattcg
     1141 tcttttttct ggtaattgac ggtgtaggtg cgggttacgt tgtccaaagt gtcggaaacg
     1201 taagtcacct cgtagccggc catttgcatg ggtacgcctt tgaacaggag tacattgttt
     1261 ttgagccgct cgctgtcgcc gccttcgatg aggccgtcca tgacgaaggt gttgatggaa
     1321 acgaaacgct tgttcagtcc ggaagccaaa attcccacaa tcatcatccc aaagccgatg
     1381 tgtgccaata ccgagcctcc gactttgagc ctgccgcgca ggaaagtgat gatgtagtcc
     1441 aagttggcta tcagtgtgaa gcaaccagca aacaacaaca gcacatactg ccaggcatag
     1501 gcgttgatcc agcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnng tttccggccc agttcatctc gcggaagcgc aaaaactgca
     1621 ctccgccgga gagcagtcct ataaaaatac cgatccagag ctggtatttg ttgtaatgcg
     1681 gaatggggtc ggtgatgacg cggcccacat acgccggatt gaaggattcc atgattttat
     1741 tgaacaccgg cagcgaggtg gaggctgtaa tgagcagcgc cgaaaacaac agcaccagac
     1801 taccgatgaa gagccagaac tccttggaaa ccaacgactc ttctggtttt ggctctggaa
     1861 tttgcctgaa ccgtgcaatg agcagtacca atcctatcaa caggaatgtg agtacaaaga
     1921 gtacgagttg cgtctccaat cccatctccg tgaaagcgtg tgccgaggtg tcgcccaaca
     1981 cgccgctgcg ggtcatgtag gtgctgtaca aaacgagcac aaagctgatg atgtagaaga
     2041 agaaggtgct gcgaatggag tagccagtgc ttttggctac cagatgcgtg tgaatgccgg
     2101 ccagcaaggt gagccagggt accagagaca tattttcaac ggggtcccag gcccaatagc
     2161 cgccgaaact gagcgcctcg taagcccatg cgccgcccat gaggatgccc gtgccgagga
     2221 tcatgccact gaacaaagcc cagggcaggg cgggtttgat ccagccctcg aagtcttttt
     2281 tccaaagtcc ggcaatggca aacgcaaagg gtacggctgt tgaagcaaag ccgaggaata
     2341 gggtaggggg gtgaatggtc atccagtagt tctggagcag cggattgagg ccgttgcctt
     2401 tgatgaggct caggtagtcg gcgttggcaa aaatgggcgc gtccatggtg ttccgaagca
     2461 agagcgtcgg gttgctgccg atgcgtacct cgccgatgta aatgcccaga atcatgatgg
     2521 aaatgaacac ctcgatgagc atcagcaccg ccagtaccgg cgattcccag tttttggcac
     2581 tcgctaccaa gaggccgccc agcaccacat gccagaacat ccaaagcaaa aaactgcctt
     2641 cctgtccttc ccaaatagaa gcaaaaatgt atttaaattc caa
//
LOCUS       DAORLF010000563         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_20835_length_2668_cov_2.469192, whole genome shotgun sequence.
ACCESSION   DAORLF010000563 DAORLF010000000
VERSION     DAORLF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20835_length_2668_cov_2.469192"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PK198_25990"
     CDS             complement(<1..1185)
                     /locus_tag="PK198_25990"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF42279.1"
                     /translation="MLRTVRFQLLLSGLLWWLLATLLQAQPVASVAAQAAFGKVRGGI
                     EGVVRDAQSGEPLPLVTLMVSGANGGGSSDLDGSFRLEGLAPGKYVITASYLGYHVTP
                     SEPIEVLPGQIVRLELRLTPEALLMGEVVVTATGRSQAVKLAPASIGVITEEQLRQRH
                     LVTFDQAFDEMPGVVVTRSSGANVQAFSIRGASEVAGGGIGNRVLLLIDGRPALSPES
                     GGALWNLVPLSSIERIEVVRGAYSSLYGSSAMGGVVNVITRKPGNEPQTRLRLNYGAY
                     DRAPRSTGYSRYNDFYSLDLSRSRSFKKWSYLVDGSWKSDDGHREKTGFDLYNLYTKI
                     TWEPAPKHRLQWSANVNRMISDAPATWLSRRQAYSVAEFKKDDFQDRREFNTDLWYQA
                     TVG"
     gene            complement(1189..1779)
                     /locus_tag="PK198_25995"
     CDS             complement(1189..1779)
                     /locus_tag="PK198_25995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42280.1"
                     /translation="MKFIKTFPVIALLAVVTALFFSSCGKDEPAKTRVYGTITIDNTN
                     LWATWKDSGEVQVTIFPKFSLDPLAGWGVVPTGTFGPNVPGGTFAVGAPYNSQNPVIL
                     TYVPGKNAYEYEIELEPGTYSALALGFRHNRVTDPSLKTATLGVHWNTPGTVSHGVVI
                     APFFNYPAPVSFDIAAGEEKEINFKADFGFVTTWYR"
     gene            1996..2205
                     /locus_tag="PK198_26000"
     CDS             1996..2205
                     /locus_tag="PK198_26000"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HRF42281.1"
                     /translation="MSNEFILKELNVLPEHLKIQVFNYIRFLKSPFVVSASQTVKKPK
                     FGFGKYKVEISEDFDAPLEDFKDYM"
     gene            2205..2438
                     /locus_tag="PK198_26005"
     CDS             2205..2438
                     /locus_tag="PK198_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRF42282.1"
                     /translation="MRILLDSHTLIWLYSGNERLSPKAGEIIENTQNECFISLASVWE
                     MAIKIKLGKMNLGVSLGNFIEDVTENGIFILPV"
BASE COUNT          685 a          648 c          696 g          639 t
ORIGIN      
        1 ccccacagtt gcctgatacc acaagtcggt gttgaattcc cgccgatcct gaaagtcgtc
       61 ttttttaaat tccgccacgc tgtaagcctg ccggcgactg agccaggtag cgggcgcgtc
      121 gctgatcatc cggttgacgt tggccgacca ttgcaggcgg tgcttgggag cgggttccca
      181 ggtgattttg gtgtagagat tgtacagatc gaaccccgtt ttctcccggt ggccgtcgtc
      241 cgatttccaa ctgccgtcca ccaaatatga ccattttttg aaactgcggc tgcggctcag
      301 gtctaaggag taaaagtcgt tgtaacggct gtacccagtt gagcgaggcg cccgatcgta
      361 agcgccatag ttgaggcgca ggcgggtttg cggctcattg ccgggtttgc gggtgatgac
      421 attcaccacg ccgcccatag cgctggagcc atagagtgat gagtaggcgc cccgcaccac
      481 ttcgatgcgt tcgatagaac taaggggaac cagattccac aacgcaccgc ccgattccgg
      541 gctgagcgca ggacgcccgt ctatcaataa tagtactctg ttgccgatgc cgccaccggc
      601 tacttccgat gcgccccgaa tagaaaacgc ctgcacattg gcgccgctgg agcgggtaac
      661 caccacgccg ggcatctcat cgaaagcctg atcgaaggtg accaaatggc gctggcgcaa
      721 ttgctcctcg gtgatgacgc ctatgctggc cggcgctaat ttgaccgcct gcgagcgccc
      781 cgttgccgtc accactacct cgcccatgag caaggcttca ggcgtcaggc gcagttctaa
      841 gcgaacgatc tggccgggaa gtacctcgat gggttctgaa ggcgtgacgt ggtagccaag
      901 ataagaagcg gttataacat atttgccggg cgccagaccc tccagtcgaa agctgccgtc
      961 caagtcggaa gacccgccgc cgttggctcc cgaaaccatc agggttacca acggcagcgg
     1021 ttctccgctt tgcgcatcgc gcaccacgcc ttctatgccg cccctcacct tcccgaatgc
     1081 cgcttgtgca gccacagacg cgacaggctg ggcctgcaac aaggtcgcca ggagccacca
     1141 cagcagcccc gacagcaaca actgaaaacg tacggtgcgc agcatgaatt agcggtacca
     1201 ggtagtgacg aaaccgaagt cggccttgaa attgatttct ttctcttcgc ctgcggcaat
     1261 gtcgaaactt accggtgcgg gatagttgaa gaaaggagca ataacaacgc cgtggctcac
     1321 cgtgccgggc gtgttccagt gtacgcccag tgtagctgtt ttgaggctgg ggtcggtcac
     1381 gcgattatgg cggaagccca atgccagcgc ggagtaagtg ccaggctcca actcaatttc
     1441 gtactcgtag gcatttttgc ccggcacata agtgagtatc accgggtttt gagaattgta
     1501 cggcgcaccc acagcaaagg tgccgccggg aacattgggg ccgaaagtgc ccgtgggaac
     1561 gacgccccag cctgccagcg gatcgaggct gaacttggga aatatggtta cctgtacttc
     1621 gccgctgtct ttccaggtgg cccagaggtt ggtattgtct atggtgatgg tgccgtaaac
     1681 gcgggttttg gccggttcgt ctttgccgca ggagctgaag aacagtgcgg tcacaacagc
     1741 caacaatgcg atgacaggaa atgtcttgat gaatttcatt ggaatacaat tgagtgttac
     1801 aaaaaaagct cttcacgagc caaactttcg gccacaaagc tatgaccgtt ccaaatttcg
     1861 tttttcacgg aattgtcaaa aaataaaaaa cgccttgcga cttgccacac gcacacttac
     1921 ttcggtatga ggagaactca acatttgagt tggataattc aagagaattt tatacttttg
     1981 gagaaaattt gaattatgtc aaacgaattc atcttaaagg aacttaacgt tttgcctgaa
     2041 catttgaaaa tccaagtgtt caattacatt cgttttttga agagtccttt tgtcgtttcg
     2101 gcttctcaaa ctgtgaagaa accaaagttt ggatttggca agtacaaggt ggaaatctcg
     2161 gaggattttg atgcgccttt agaggacttt aaagactaca tgtaatgagg atactattgg
     2221 atagtcatac gcttatttgg ctttattcgg gcaatgaacg acttagccca aaagcaggag
     2281 aaataataga aaatactcaa aatgagtgct tcataagttt ggcaagcgtt tgggaaatgg
     2341 caattaaaat caaattaggt aaaatgaatt tgggtgtttc tcttggaaat ttcatagaag
     2401 acgtaacgga aaatggaatt tttattttgc cggtctagat ggttcatatc ctaaaaacgc
     2461 aagaattgga atttcaccat aaagacccat ttgacaggtt gctattttcg caagcaatta
     2521 ccgaaaacat ggttttactt tctgcggacg aaatagcaga ttcatatttt gcaaatgaaa
     2581 gtgtaaaacg aatttggtag aaaaatagac acatagtagt ggggcaaaaa taaagtgttc
     2641 attttcttta ggccaaagga ttagataa
//
LOCUS       DAORLF010000564         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21027_length_2650_cov_1.739114, whole genome shotgun sequence.
ACCESSION   DAORLF010000564 DAORLF010000000
VERSION     DAORLF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21027_length_2650_cov_1.739114"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK198_26010"
     CDS             complement(<1..367)
                     /locus_tag="PK198_26010"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF42283.1"
                     /translation="MERRNFLGAAGLLAGLAALPEIGKANSIWDETPGTPTQALLLPP
                     MPPLDHKGSMDIRVWLRSSMTGGVYSCVECAVAPKTMGPAPHWHKNLDELMLVLEGTA
                     GVMVEGEHVEVPAGGGHLRP"
     gene            complement(536..>1059)
                     /locus_tag="PK198_26015"
     CDS             complement(536..>1059)
                     /locus_tag="PK198_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42284.1"
                     /translation="TKTSTTTDKEGNTTTQTTTTDGQGHSHTQTHSHDKNGNTHNPPQ
                     PPPGDDGSRSNPEGNSSDSPPVKPGEIGKGRPGHPMDHVVPPGSWQKDNGEWQGEQPP
                     MYRDMLAGILLVLNPYAQTGHGDAADPDAQREDLLRLLSSLEITSSASGEDFVHPKAK
                     AGLLEAMLGKRGG"
     assembly_gap    1060..1214
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1215..1956)
                     /locus_tag="PK198_26020"
     CDS             complement(<1215..1956)
                     /locus_tag="PK198_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42285.1"
                     /translation="MNTIENWEEKLRETSPNGYIAVKIFADDYIRKAKEYERTGRELL
                     DGAEKAVHGDAGQIYSDGVHKGADWGGKAAGGATAAVVVAAGAALGAALAGPVGAVLG
                     GWLLSGVAGMLGGVVQGFVTNLIESAGRALAGALGLDFHTHGKTPGGGTWNRTDHPNG
                     DWERTEIGPDFNVTESQQGGAFSQTIVGKGAHAGYRSHFTDDGKGNAHFEAVDKAGVL
                     HNFNQDDSGSAWGRAGTNDTDKSNGKSGG"
     gene            complement(2139..2585)
                     /locus_tag="PK198_26025"
     CDS             complement(2139..2585)
                     /locus_tag="PK198_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42286.1"
                     /translation="MLKFIFRLLFVALGLWITYTFGRDAVEPVLYRLSGTTVEGRIIG
                     FLAGRKSPSVQPEPTAIRKGRRIARRPVFRYPTAVGATDSLDGRSSTGGFLIFGHFDL
                     HEQVTVVMPAENPQNAHIFSLRLIGMAFVVALFGLFLIWMGITGKG"
BASE COUNT          474 a          773 c          666 g          582 t
ORIGIN      
        1 gggggcgcaa atgccccccg cctgccggca cctcaacgtg ttcgccttct accatgacgc
       61 ccgcggtacc ttccagcacc aacatcagtt cgtccagatt tttatgccaa tgtggcgccg
      121 gacccatggt tttaggcgct acggcacatt ctacacaaga atacaccccg cccgtcatgg
      181 aggagcgaag ccacacgcga atatccatac tccctttgtg atctaatggt ggcatgggcg
      241 gcaggagcag cgcctgagtg ggcgtgccgg gcgtttcgtc ccagatgctg ttggccttgc
      301 cgatctcggg aagggccgcc aaacctgcca acaggccggc cgcacccaaa aaattgcgtc
      361 tttccatgtt gatgaaatgt attgaaaatg ttgagttaat cacttaaaat accgctggcg
      421 cgagatggaa tctcattttt ccatgcactg cgcattagcg tcaaccaaga tgggctacta
      481 agaagtggaa ccgccaactg catactgcca actggcgcaa gattccctgc tgctcctacc
      541 cgcctctctt ccccagcatc gcctccaaca aacccgcctt cgccttggga tgcacaaaat
      601 cttcgccgct tgcactgctc gttatctcaa gactactcaa caaacgcagc aagtcttctc
      661 gttgtgcgtc cgggtcggcg gcgtcgccat gccctgtttg agcataagga ttgagcacca
      721 gcaaaatacc cgccagcata tcacggtaca taggcggctg ctcgccctgc cattcgccgt
      781 tgtctttttg ccacgatccc ggcggtacca catgatccat ggggtggccg ggccgccctt
      841 ttccgatttc gccgggcttc acgggcggac tgtctgaact gttgccttcc gggttgctcc
      901 ggctgccgtc gtcaccgggc ggcggctgcg gcggattgtg ggtgttgccg tttttgtcat
      961 ggctgtgggt ttgagtatgc gagtgtccct gcccgtcggt agtggtggtt tgggtagtgg
     1021 tattgccctc tttgtctgtg gtggtcgagg ttttggtttn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnggccac cgcttttgcc gttcgatttg tcggtatcgt tggtgccggc
     1261 cctgccccag gccgaaccgg aatcatcttg gttgaaattg tgcaaaacgc cggccttatc
     1321 caccgcttca aaatgagcat tgcctttgcc gtcgtcggta aagtgagagc ggtagcccgc
     1381 gtgtgcgcct ttgcctacaa tggtttggct gaaagcgccg ccctgctgcg attccgtcac
     1441 attgaaatcc ggcccgattt cggtgcgctc ccagtcgccg ttggggtggt cggtgcgatt
     1501 ccaggtaccg ccgccgggag tcttgccgtg tgtatggaag tctaagccca gcgcgccggc
     1561 caaggccctg cctgccgact cgatgagatt ggtcacaaaa ccctgtacca ctccgcccag
     1621 catccccgcc acgccactga gcaaccaccc gcccagcaca gctcccacag gccccgccag
     1681 cgctgccccc agcgccgcgc ccgccgccac taccactgcc gccgtagctc cgccggctgc
     1741 tttacctccc caatcggcgc ctttgtgtac gccgtcggaa taaatctgac cggcatcgcc
     1801 atgcacggct ttttcagcgc cgtccagcag ttcgcgcccg gtgcgctcgt attctttggc
     1861 cttccggatg tagtcgtcgg caaatatttt caccgcgatg tagccgttgg gagatgtctc
     1921 ccggagtttt tcctcccagt tttcgatggt gttcattgat atttattgtt caaggtttcg
     1981 ggtttcatcc gccgtaccac tcctgtggcg tgggaaggcg ggttcgaggt tccgggttcc
     2041 accgtggatg aactgccgaa gtcagattca ccctctccat tggcaagata aactgcgcag
     2101 cagattccgg caaagccgat aaactgccga aggcagattc atcccttccc cgttattccc
     2161 atccagatca aaaacaggcc gaacagcgca acaacgaagg ccatgccgat caatcgcagg
     2221 ctgaaaatgt gcgcattttg agggttttca gccggcatca ctacggtcac ttgctcatgc
     2281 aaatcgaaat gcccgaagat caaaaatccg cccgtgctgc tgcgcccgtc taagctgtcg
     2341 gtagcaccca cggccgtcgg atacctgaac acaggccgcc gggctattct tctgcccttt
     2401 cggatggccg tcggttcggg ctgcacggag ggcgactttc ggcccgccaa aaaaccgatg
     2461 atgcgccctt cgaccgttgt gccactcagg cgatacagca ccggctctac cgcgtcgcgg
     2521 ccaaaggtgt aggtaatcca aagccccagg gctacaaaca agagtcgaaa aatgaatttc
     2581 agcatggttc aataaatttc ggattcctga tttcggattc ctgatttcgg attcctgatt
     2641 tcggattcct
//
LOCUS       DAORLF010000565         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21040_length_2649_cov_2.461064, whole genome shotgun sequence.
ACCESSION   DAORLF010000565 DAORLF010000000
VERSION     DAORLF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21040_length_2649_cov_2.461064"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..383)
                     /locus_tag="PK198_26030"
     CDS             complement(123..383)
                     /locus_tag="PK198_26030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42287.1"
                     /translation="MEFVLQEARREAERKRIEAEGVRDAQNIIAQGLSPILVQYKSIE
                     AFLELAKSSNAKVIISDGKMPMMIDMGAETKTTPASLNTGRN"
     gene            complement(411..950)
                     /locus_tag="PK198_26035"
     CDS             complement(411..950)
                     /locus_tag="PK198_26035"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HRF42288.1"
                     /translation="MKKLLYLLPVLLAATFLLPSCTVIRQGEVGVKRTFGKYKDQPYT
                     EGLKIFNPFVTTFVRVPTQTNNLEVSLNIPSREGLTIQSEVSILYNVVPRKAPDLLRN
                     IGRNYESSVILPVFRSAVADVTSRFDAKDMHTGERGVIEVAIRDQMTKLLQDKGIIIE
                     AVLLKSIILPRRLSRAIEE"
     gene            complement(991..1815)
                     /locus_tag="PK198_26040"
     CDS             complement(991..1815)
                     /locus_tag="PK198_26040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF42289.1"
                     /translation="MSGFSIELSINDKLYLRDPQRSEVGRRILRHSILLMDEIGLESF
                     TFKKLAEHADTTEATIYRYFESKHLLLLYLINWYWAWTAVQIDMAVQNIEDAEDRLRR
                     AIKAVISASRRNLSVDFIDEDVLHRIVVVEGTKAYHNKDVDAQNKEGFFMAYKALSAQ
                     LAKLILAVNPNFQYPKALATNILEMAGNHVYFAEHLPRLTDVQAGPNLADDVVRLLES
                     FTMKLIKVSSEKPIYTTTSPSAGGKRDYPGESGPDRFSQTGAQQYPRDQPMQHPPE"
     gene            complement(1907..>2325)
                     /locus_tag="PK198_26045"
     CDS             complement(1907..>2325)
                     /locus_tag="PK198_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF42290.1"
                     /translation="YKAFKYRGMYQPQTNLRAWLMTIMRNTFINNYRQRKRRQTITDD
                     TPNTFLLDSAAPAVLNTAPGAITFEELYSEVEKLEDWVRIPFLMHYQGFKYEEISNEL
                     QIPLGTVKSRIFFARKKLQEAVRVMYKNADLSELLN"
     assembly_gap    2326..2347
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          645 a          621 c          645 g          716 t
ORIGIN      
        1 ttacttatct aatcctttgg cgaaaatgac cactttattt ttttatcgcc ccttacataa
       61 aaaaaggatt gcagcttttg aaatacaagc cgcaatcccc tctacaacaa ttactctttg
      121 tcttagtttc tgccggtatt cagcgaggcc ggcgtcgttt ttgtctcggc gcccatatct
      181 atcatcatcg gcattttacc atctgaaatg atgactttgg cattgctcga cttggccaat
      241 tccaaaaatg cttcgataga tttgtattgt accaatatcg ggctaagccc ctgtgcgatg
      301 atgttttgtg catccctgac gccttcggct tctatgcgtt tgcgttctgc ttcgcgcctg
      361 gcctcctgca gcacaaactc catgcgctga gcggcttgct ctgcctccaa ttactcctca
      421 atggcacggg aaagtctgcg cggcaggatg atgctcttga gcagtacggc ctcaatgata
      481 atacctttgt cttgcaacaa cttggtcatt tggtcgcgga tggcaacttc tatgacgccc
      541 cgctcaccgg tgtgcatgtc tttggcatcg aaacgggaag ttacatccgc cactgccgag
      601 cggaaaacag gcaggatgac gctgctctca taattccggc cgatgttgcg cagcagatcg
      661 ggcgctttgc ggggaaccac attgtacagg atcgaaacct ccgattgaat ggtcagtccc
      721 tcccgcgaag ggatgttgag cgatacctcc agattgttgg tttgcgtcgg caccctcaca
      781 aaagtggtaa caaatggatt gaaaatcttc aacccctcag tatagggctg gtctttgtac
      841 ttgccaaaag tgcgtttaac gccaacttcg ccctggcgga tgacggtgca agagggcaat
      901 aaaaatgtgg ctgccagaag taccggcaga agatacaata gcttcttcat tttttatttg
      961 attttgaggt aattaaacaa taatcaagaa ctactccggc ggatgctgca ttggctgatc
     1021 tcgcgggtac tgttgggcgc cggtttgcga aaaacgatcc ggcccggatt caccgggata
     1081 gtctcgtttt ccgccggctg aaggtgaagt ggtggtgtaa attggttttt cggaggaaac
     1141 tttgatcagt ttcatggtaa atgattccaa caatcggacg acatcatcgg caaggttcgg
     1201 gccggcttgt acatcggtga gccggggcag gtgttctgca aaatacacat gattgccggc
     1261 catttctaaa atattggttg ccaatgcttt gggatattga aaattaggat tgaccgccag
     1321 tatcagcttt gccagttggg cgctcaatgc cttgtaagcc ataaaaaagc cttctttgtt
     1381 ttgcgcgtcc acgtccttgt tgtggtaggc tttggtgcct tccacgacca caatgcggtg
     1441 cagtacgtcc tcatctatga agtcaacgct caggttgcgg cgggaggcgc tgatgaccgc
     1501 tttgatggct cgccgcagcc ggtcttcggc atcttcaatg ttttgtacag ccatgtctat
     1561 ttgcacggca gtccaggccc aataccaatt gatgaggtat aacaacagta ggtgtttgct
     1621 ctcgaaatac ctgtaaatgg tggcttccgt ggtgtcggca tgttcggcca gcttcttgaa
     1681 ggtgaagctc tccaatccga tttcatccat caacaaaatg gagtgccgga gaatgcggcg
     1741 tcccacctcg gagcgctgcg gatcgcggag gtacaacttg tcgttgatac tcagttcgat
     1801 agaaaaaccg ctcatatttt tgtaatattt actgattgtg ttgcacccgc tgagttgccg
     1861 gttttggaaa aattcgagtc agataaaacc aatagctttg cccgaattaa ttcaacagtt
     1921 ccgacaaatc tgcattctta tacatcaccc gtacagcctc ctgcaacttc ttacgggcaa
     1981 agaaaatccg gctcttgacg gtgcctaaag gaatctgcaa ctcatttgaa atttcctcgt
     2041 atttgaaacc ctggtagtgc atcaaaaaag gaatgcgcac ccaatcttcc aacttttcca
     2101 cttcggaata cagctcttca aaggtgatgg cgcccggcgc ggtattgaga acggcagggg
     2161 ccgcgctgtc aagaaggaag gtgttcggcg tgtcgtccgt gatcgtctgg cggcgcttcc
     2221 gctggcggta gttgttgata aacgtgttgc gcatgatggt catcagccag gcgcgcaaat
     2281 tggtttgcgg ctggtacatg cccctgtatt tgaatgcctt atatgnnnnn nnnnnnnnnn
     2341 nnnnnnnaat cctgtgcgtc gttctcgttg ttggtaagcc gaactgcaaa ggaatgtaag
     2401 gtgccctgaa actggctgaa atgatgggtg aactccagat gtgacatggc caatcgtttt
     2461 atattggtga ttcaatacag caaagatagt aataaataat agttttacta tctttttctt
     2521 gaataaaact aaagcacatg aaagaagttc actcgattat ggaaaaaatt aaaaattttc
     2581 cggctgaaaa aagaatccca gccggaacag caagcagggc aaacgcatca aaaccaagaa
     2641 tgacctcgg
//
LOCUS       DAORLF010000566         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21186_length_2636_cov_2.033320, whole genome shotgun sequence.
ACCESSION   DAORLF010000566 DAORLF010000000
VERSION     DAORLF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21186_length_2636_cov_2.033320"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..>273)
                     /locus_tag="PK198_26050"
     CDS             complement(18..>273)
                     /locus_tag="PK198_26050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42291.1"
                     /translation="VHVLQGGFVVLKFYRHDVVVLWFCGFMVCWFVGLLVLSAELRHR
                     RGKTGFMILWMRAFVASFFSPRSICRSLRRYVCNRRANAE"
     assembly_gap    274..341
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <342..824
                     /locus_tag="PK198_26055"
     CDS             <342..824
                     /locus_tag="PK198_26055"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRF42292.1"
                     /translation="VEVKKLKHEADEREQLLASRMAGYHNVLRLWEEFDPSVVQVEQV
                     LLDQRKIAGVLIPVLRDVSFRVEPFSLFNKPHWWPDGVATLKELARIGIEKQVLLRAM
                     SLLDRARKKTTQKVNLYEKVQIPGYEEAVLKIKRFLEDEENLSKSAQKIVKKRQEALG
                     "
     gene            922..>1949
                     /locus_tag="PK198_26060"
     CDS             922..>1949
                     /locus_tag="PK198_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42293.1"
                     /translation="MIVPMRKYAFLVHPQDYQNFLEDLRAVGVLHIQTQKDLPPPEQL
                     SEHERRLKDMESAIRLVEARRKEDAEMPAAQALSTDEDPARRIWSLQEALDSDRQKLQ
                     VIRKEQQNMAPWGNFSSATLEHIAQAGWQCSFYTCPQRKFQPEWASDRYLFVINELPP
                     DAHFVVLHPPGARPDLDAELLPAPARDPEALRAAAAELETHIAALEAQLDDFAAQLPA
                     LDGVRNQLLEHTDTEQALRHTITDAADGAIMVLEGFVPRPVEDQLQKVFENQPIVFWA
                     ENPIPEDQPPVLLKNGRFSKYFEPISKLFALPSYGELDLTPFFAPFFMLFFGFCLGDA
                     GYGVVILVG"
     assembly_gap    1950..2005
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2006..>2636
                     /locus_tag="PK198_26065"
     CDS             <2006..>2636
                     /locus_tag="PK198_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42294.1"
                     /translation="TLTHFMGAATILFGVLTGTVFGINLLGEEYAWLGDTRRIMIDSK
                     QAFNLALVLGLIQTIFGLALQSINRIKQFSLAYGMPPIGWILLLLSLLDIGMLKMAAP
                     FSTWTAWAGVALIVLFSMPKGHILSRIGKGLWDLYGITGFFGDLLSYVRLFALGISSA
                     ILGFVINDIAIQIKGFMPVIGPILFVIFLIVGHGANLLISSLGSFVHPMR"
BASE COUNT          608 a          773 c          608 g          523 t
ORIGIN      
        1 ctattttgat gaaatgctca ttcagcgttg gctcggcgat tgcagacata tctccgcaat
       61 gatcggcaaa ttgatcgcgg cgagaaaaag ctcgccacaa aagccctcat ccacaaaatc
      121 ataaaacccg tcttccctcg cctgtggcgt agttcggcgg ataaaaccaa caaaccaaca
      181 aaccaacaaa ccataaaacc acaaaaccac aaaaccacaa catcgtgcct gtaaaatttc
      241 aatacaacaa aacctccctg caggacatga accnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtggaggtc aaaaaattga
      361 aacacgaagc cgacgaacgc gagcaattgc tggccagtcg catggccggg taccacaacg
      421 tcctgcgcct ctgggaagag tttgacccct cggtcgtgca ggtagaacaa gtgttgctcg
      481 atcagcgcaa aatcgccggc gtgctcattc ccgtactcag ggacgtatcg ttccgggtag
      541 agccgttcag cctgttcaac aagccgcact ggtggcccga cggcgtggcc acgctcaagg
      601 aattggctcg catcggcatc gaaaaacagg tattgttgcg cgccatgtcg ctgctcgacc
      661 gcgcccgcaa aaagaccacg caaaaagtga atctttacga aaaagtacag attcccggct
      721 acgaagaggc cgttctaaag atcaagcgct ttttggaaga cgaagaaaac ctctccaaat
      781 cagcgcaaaa aattgtaaaa aaacgccagg aggcattggg ataaaaccgc aaaaccgcaa
      841 aatgtcaaat atcaaatcta aaaatatccg cacgcttagc atccccccct tgggagttag
      901 gaagccaaaa tccatcgcgc catgatcgtg cccatgcgca aatacgcctt tctggtacat
      961 ccgcaagact accagaactt cctcgaagac cttcgggcgg tgggcgtatt gcacattcag
     1021 acccaaaaag acctgccgcc gccggagcaa ctcagcgagc acgagcgcag gctcaaagac
     1081 atggaatcgg cgattcgcct cgtggaagcc cgccggaagg aagacgccga aatgcccgct
     1141 gcacaagccc tctccaccga cgaagacccg gcgcgccgca tctggtcatt gcaggaagcc
     1201 ctcgattcag atcgccagaa gttgcaggtc atacgcaaag agcaacaaaa catggcaccc
     1261 tggggcaact tctccagcgc cacgctcgaa cacattgctc aggctggctg gcaatgtagt
     1321 ttttacactt gcccgcagcg gaaattccaa ccggaatggg cctctgatcg ctacctcttt
     1381 gtcatcaacg agttgccgcc cgatgcgcac tttgtggtgc tgcacccgcc cggcgcgcgc
     1441 cccgacttag acgccgaact cctgcctgcg cccgcccgcg atcccgaagc cctgcgcgcc
     1501 gccgccgccg aattggaaac acacatcgcc gctttggagg cacaattgga cgacttcgca
     1561 gcccagcttc ccgctttaga cggcgtgcgc aatcaactct tggaacatac cgacaccgaa
     1621 caggccctcc gccacaccat caccgatgcc gccgacggcg ccatcatggt tttggagggt
     1681 tttgtacccc gcccggtaga agaccaattg cagaaggtat ttgaaaatca gcccattgta
     1741 ttctgggccg aaaaccccat tcccgaagac cagccgccgg tattgctcaa aaacgggcgt
     1801 ttctccaaat acttcgagcc gatcagcaag ctttttgcac tgccttctta cggcgagttg
     1861 gacctgacgc cctttttcgc accgttcttc atgctctttt tcggcttctg ccttggcgat
     1921 gcgggctatg gcgttgtcat cctggtcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnngacgc tgacacattt catgggcgcc gccaccatac
     2041 tcttcggcgt tctcaccggc accgtgttcg gcatcaatct gctgggcgaa gaatacgcct
     2101 ggctgggcga tacccgccgc atcatgatag acagcaagca agccttcaat ctggctttgg
     2161 tgctggggct gatccaaacg attttcgggc tggccctgca atccatcaac agaataaagc
     2221 agttcagctt agcttacggg atgccgccga taggctggat tctcctgttg ctcagcctct
     2281 tagacatcgg gatgctgaaa atggccgccc cattttccac ctggacggcc tgggcaggcg
     2341 tggccctcat cgtcctgttc agtatgccca aaggtcatat cctgagccgc atcggcaagg
     2401 gcttgtggga tttatacggc atcaccggct ttttcggcga cctcctcagc tacgtccggc
     2461 ttttcgcgct cggcatttcc agcgccatcc tcggcttcgt catcaacgat attgccatcc
     2521 agatcaaagg attcatgccg gtcataggtc ccattttgtt cgtcattttt ctcatcgtgg
     2581 gacacggcgc caatctcttg atttcttcgc tcggttcctt cgttcacccc atgcgt
//
LOCUS       DAORLF010000567         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21421_length_2613_cov_2.016810, whole genome shotgun sequence.
ACCESSION   DAORLF010000567 DAORLF010000000
VERSION     DAORLF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_2613_cov_2.016810"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    237..305
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<306..1836)
                     /locus_tag="PK198_26070"
     CDS             complement(<306..1836)
                     /locus_tag="PK198_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRF42295.1"
                     /translation="MSKKEKFTEIITSGYTFKGDSIVLGAAMLDGETINGLQVKVPLR
                     TLNRHGLIAGATGSGKTKTLQIIAEQLSSKGVPVLLMDIKGDLSGIAAPGAANPKIEE
                     RHLKIGEPFVPGTSPVEFLSLSEEKGARLRATVTEFGPVLFSKILDLNDTQGGVVAVV
                     FKYCDDNKLPLVNLADFKKVLQYITGPGKEEIEGDYGQVSSATVGIIMRKIIELEHQG
                     AEAFFGERSFDPGDLCRFDENGKGIVSVIRLTDIQDRPKLFSTFMLQLLAEIYSTFPE
                     EGDLDQPKLCIVIEEAHLVFAEASKALLNQIEAIVRLIRSKGVGVFFVTQSPADIPEN
                     VLAQLGLKVQHSLRAFTAKDRKSIKLAAENYPLTDYYDVAQLITELGIGEAFITALNE
                     KGIPTPLAHVLLRAPQSRMDVLTDSEIDSIIRRSQIARKYNEVIDSHSAQEILTQKMD
                     QIAREQEEEEYRKQREKEMAELERSRAKTTSRTTSSRTTTSRPRAQQSTFEQIVNSHT
                     TR"
     gene            complement(1840..>2169)
                     /locus_tag="PK198_26075"
     CDS             complement(1840..>2169)
                     /locus_tag="PK198_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRF42296.1"
                     /translation="GPVLSMPVKGTIGENEVSIPAAFFKVLLDADSSNPKAIAFLVPN
                     EISFDPLTKYVVSVDEVEKRTGLDFFPDLLDEDTEKQLESRFNVDLWEFSKQKFQQRV
                     NAWNKQQ"
     assembly_gap    2170..2372
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2373..>2613)
                     /locus_tag="PK198_26080"
     CDS             complement(<2373..>2613)
                     /locus_tag="PK198_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRF42297.1"
                     /translation="PETPAADNRPKSELPDFLPRSSTRQIIDHQYFWLSYDEEHEQAE
                     WTAHILSKENLEKPWVDRQDDFRPDDKIKTGSATPN"
BASE COUNT          445 a          589 c          693 g          614 t
ORIGIN      
        1 tcagcggttt tcccgatgcc tgcggcgcct attgcgatgg cgcccgtgcc gagcaggctt
       61 tttttgataa atgattttct gtccatacct aagctttttg gtgatggcac aaagttgggg
      121 tcaaaccgga cttgaaaaca ttgaattaga ttaagaaaaa agtgtgggga ttcagttagg
      181 agaccttgca ggggtttgga aaacctgcaa ggtctcattt atactatctc ctccggnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnngacgg gtggtgtgac tgttgacgat ttgttcaaaa gtgctttgct gggcgcgcgg
      361 gcgcgaggta gtggtgcggc tggatgtagt gcgcgaagta gtttttgcgc ggctgcgctc
      421 caattcggcc atctcttttt cgcgttgctt ccggtattcc tcctcctcct gctcgcgggc
      481 gatctgatcc attttttgag tcagaatttc ctgagcgctg tgcgaatcaa tcacctcgtt
      541 atacttccgg gctatctgcg atcggcggat gatgctgtct atctcgctgt cggtcagcac
      601 atccatgcgg ctttgcggcg cgcgtagcaa cacatgcgcc aacggcgtgg gaatgccttt
      661 ttcgttgagt gcagtaatga acgcttcccc gatacccagc tcggtgatga gttgcgccac
      721 gtcgtagtag tcggtcagcg ggtagttttc ggcggcgagt ttgatggact tgcggtcttt
      781 ggccgtgaag gcccggagcg agtgctgcac cttgaggccc aactgcgcca gtacgttttc
      841 cggtatgtcg gccgggcttt gagtgacaaa aaacacgccc acacctttgg aacggatgag
      901 gcggacgatg gcttcgatct ggttgagcag agctttcgac gcctccgcaa aaaccaagtg
      961 cgcctcttcg atgacgatgc agagtttggg ctgatcgagg tcgccttctt ccgggaaggt
     1021 ggaataaatc tcggccagca attgcaacat aaaagtagaa aacaacttgg gccggtcctg
     1081 aatatcggtg agacgaatga ccgacacgat gccctttccg ttttcatcga accggcacag
     1141 atcgcccgga tcgaaggagc gctcgccgaa gaacgcctct gcgccctgat gttccaactc
     1201 gatgattttg cgcatgatga tgcccaccgt cgccgacgat acttgcccgt agtcgccctc
     1261 aatttcctct ttgcccggcc cggtgatgta ctggagcact tttttgaaat cggccaggtt
     1321 caccaaaggc agtttgttgt cgtcgcagta tttgaatact actgccacca cgccgccttg
     1381 tgtgtcgttg aggtcgagga ttttggaaaa caaaaccggc ccgaattcgg tgacggtagc
     1441 gcggaggcgg gcgccttttt cttccgagag gctgaggaat tccaccgggc tggtgccggg
     1501 tacaaacggc tcgccgattt tgaggtggcg ctcttcgatt ttcgggttgg cggcgccggg
     1561 ggccgcaatg ccgctgaggt cgcctttgat gtccatgagc agcacgggca cgcctttgga
     1621 ggagagttgc tcggcgatga tctggagcgt tttggttttg cccgaacccg tagcgccggc
     1681 aatgagtccg tggcggttga gggtgcgcag cggcactttt acttgcagtc cgttgatggt
     1741 ttcgccgtcg agcatggcag cgccgaggac gatggagtct cctttgaaag tgtagccgga
     1801 ggtaatgatt tcagtgaatt tctctttctt cgacatagtt tattgttgtt tgttccaggc
     1861 gttgacgcgc tgctggaatt tctgtttgct aaattcccac aaatctacgt taaaacggct
     1921 ttccaattgt ttttcggtgt cctcgtccag caaatcggga aaaaaatcca gcccggtgcg
     1981 cttctccacc tcgtctaccg acacgacgta tttcgtgagc ggatcaaaac tgatttcgtt
     2041 gggcaccaaa aacgcaatgg ctttgggatt ggagctgtcg gcatcgagca gcactttgaa
     2101 aaacgccgcc ggaatggaca cctcgttttc gccgatggta cctttgacgg gcatggacag
     2161 caccggcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgttgggc gtggccgagc cggtttttat
     2401 cttgtcgtcg ggccggaaat cgtcttgtct gtccacccag ggtttttcca gattctcttt
     2461 gctgaggatg tgcgccgtcc attcggcctg ctcgtgttct tcgtcataag acagccaaaa
     2521 gtattggtgg tcaatgattt gccgggtaga ggagcgcggc aggaagtccg gcagttcgct
     2581 tttgggacgg ttgtcggcag cgggtgtttc ggg
//
LOCUS       DAORLF010000568         2594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21628_length_2594_cov_2.222922, whole genome shotgun sequence.
ACCESSION   DAORLF010000568 DAORLF010000000
VERSION     DAORLF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21628_length_2594_cov_2.222922"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PK198_26085"
     CDS             complement(<1..1994)
                     /locus_tag="PK198_26085"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRF42298.1"
                     /translation="MKKPYTLTALVAMTLVFPLFIWGQTTHHYEIRFPKDAQANCTLP
                     VPDTILTEEIANCDLLAVSVHDETFSASGDECYKILRTYRVINWCEYDGQSPAVIVSR
                     DEDCDGIPGDEHVWVLVKPNGTVYFDRDNSEGNGVPAAFTKGSSCDGLSNPAGHWVNS
                     AIKPAITSRGFWQYAQFIKVYDNVDPVISVTTPAPFCSYDSPTDADPICEGPVTFPFS
                     VSEVCTPDDVTIKVFLDLYNNGVFPYDHTVRGQPNGTLSGSTQIFNILGSYPNYSIAS
                     TAPQGLPIGTHKFEIHAEDGCGNVAAVHVLVEVRDCKAPTPICYNGLTLTLMPVDTDN
                     DGLPDDGMAEVWASDFIASPVVDCSPPVRYSIRGEGQQPDVTRLSLVFDCEDHDATNG
                     SDGTQIVVFIDAWDSVGNRDFCETYILLQDQSGVCPGPAMGSISGMLRRETGSPVQGL
                     DVQVVGGMQQSMTSNTSGQYAFQQLPAGQAYTVQGLSSYDYLLDCVSTFDLILIQRHI
                     LGIQPLNSPYKIIAADVNRSGSVTILDLILLQMEILAIQNPAEQLVWRFIPADYEFPN
                     PVNPWAEVFPEQIVFEDLWGNHPDQDFIVIRVGDVTNCSSPNLNEVEWRNTPEGILWI
                     DLPDAALRPNEMFTVTLKNKDLSRVSGLQFGLRFNDAALSL"
     gene            2107..>2594
                     /locus_tag="PK198_26090"
     CDS             2107..>2594
                     /locus_tag="PK198_26090"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF42299.1"
                     /translation="MTDSDYISHFTDLSGGRLRVHRWGGGVRLLLCLHGYGDTGERFG
                     GLARALGNEYTVIAPDLPWHGATEWKKKSFDAWDVQEMAESLMALENKADCILLGHSW
                     GARLVMSALPLLSEQVSSAYFIAPGGFDPASRWAGEKLPLWLRNALIYSVEHHTGRWL
                     AFA"
BASE COUNT          585 a          668 c          741 g          600 t
ORIGIN      
        1 aggctcaatg cagcgtcatt aaacctcagc ccgaattgga gacccgaaac ccggctcagg
       61 tctttatttt ttaaagttac cgtgaacatc tcgttggggc gcagggccgc gtcgggcagg
      121 tctatccaga ggatgccttc gggtgtgttg cgccattcga cttcgttcag gtttggcgag
      181 ctgcaattgg tgacatcacc caccctgata acgatgaaat cctgatcggg gtggttgccc
      241 cataaatctt cgaatacaat ctgttccggg aaaacctctg cccagggatt gacaggattg
      301 ggaaactcat aatctgccgg aatgaagcgc caaaccaatt gctctgctgg gttttgtatg
      361 gccaaaattt ccatttgcag caggataagg tcgagaatcg ttaccgagcc ggagcggttc
      421 acatcggcag cgatgatttt gtaagggctg ttcaaaggtt ggatgcccaa tatgtgtctc
      481 tgtatcaaaa tgaggtcaaa agtggacacg caatcaagca ggtagtcata agaacttaga
      541 ccttgtactg tgtacgcttg ccctgccggc agttgttgga aagcatattg cccgctggta
      601 ttgctcgtca tgctttgctg catacctcca acaacctgta catccaatcc ctgaacaggc
      661 gaaccggtct cgcggcgcaa catccccgaa atgctcccca tagccggccc cggacatacg
      721 ccgctctggt cttgaagcag tatataagtc tcacaaaaat cgcgattgcc gacgctgtcc
      781 caggcatcaa taaaaacgac aatttgagtg ccgtcggaac cattcgtggc atcgtggtct
      841 tcgcagtcaa aaaccaaact gagccgggtg acgtcgggtt gctgcccctc gccgcgaatg
      901 gaatagcgca cgggcggcga acaatctaca acggggctgg caataaagtc ggaagcccaa
      961 acctcggcca tgccgtcatc gggcaaacca tcgttgtcgg tatccaccgg catgagggtg
     1021 agcgtaaggc cgttgtagca aatcggggtg ggcgctttgc agtcgcgtac ttccaccaga
     1081 acatgcacgg cagccacatt gccacaaccg tcttcggcgt gaatttcaaa cttgtgcgtg
     1141 ccgatgggca gcccctgtgg cgcagtgctt gcgatgctgt agttggggta gcttcccaat
     1201 atgttgaaaa tctgggtgct gccgctgagc gttccgttgg gttggccgcg cacggtgtgg
     1261 tcgtaaggaa aaacgccgtt gttgtacagg tccaaaaaca ctttgatcgt tacatcgtct
     1321 ggcgtgcaaa cttctgagac ggagaagggg aaagtcaccg gtccttcaca aatgggatcg
     1381 gcgtcagtag gggagtcgta gctgcaaaac ggcgccggag tggtcactga aatgacggga
     1441 tcaacattgt cgtaaacctt gatgaattga gcgtactgcc agaatccccg cgaggtaatg
     1501 gccggtttga tggcggaatt gacccagtgc ccggcgggat tgctcagccc atcgcaggaa
     1561 ctacctttgg tgaaggctgc cgggacgccg ttgccctccg agttgtcgcg gtcgaaataa
     1621 acagtgccgt tgggttttac caaaacccat acatgctcat cgccgggaat gccgtcgcag
     1681 tcttcgtcgc ggctcacgat gaccgccggc gactggccgt cgtactcgca ccagttgatg
     1741 acgcggtagg tgcgcaaaat tttgtaacac tcatcgccgg aggcagagaa ggtttcgtca
     1801 tgcacgctga cggcgagcag gtcgcagttg gcaatttctt ccgtcaggat ggtatccggc
     1861 acaggcaggg tgcaattggc ttgcgcgtcc ttggggaagc gaatttcgta atggtgggtg
     1921 gtttgccccc agatgaacag gggaaaaacg agggtcatgg ccactaaggc cgtcagagtg
     1981 taaggctttt tcatataccg cgtagtttta gatttctgca aaattaactt cattcgctga
     2041 aattatcaac ccgcagttca aactttaata cacttgctct atcattgtac ccaaatagga
     2101 gcgactttga cggattccga ttacatctca cattttaccg acctctcagg cggtcgcctc
     2161 cgcgtacaca ggtggggtgg gggagtgcgc ctgctgcttt gcctgcatgg ctacggcgat
     2221 acgggcgagc gatttggagg cctggcacgg gctttgggca acgagtacac ggtcattgcg
     2281 ccggacttgc cctggcatgg cgccaccgaa tggaaaaaaa aatcattcga tgcctgggat
     2341 gtacaggaaa tggcggaatc gctgatggcc ttagaaaaca aagccgactg catattgttg
     2401 ggccacagtt ggggcgcccg cctggtcatg tcggcactgc cattgttgtc ggagcaggta
     2461 tcttctgcgt attttattgc gcccggcggc ttcgatccgg cttcgcgctg ggccggcgaa
     2521 aaactcccgc tttggttgcg caatgcgctc atttacagtg tagaacatca taccggcagg
     2581 tggctggctt tcgc
//
LOCUS       DAORLF010000569         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_21763_length_2583_cov_1.753165, whole genome shotgun sequence.
ACCESSION   DAORLF010000569 DAORLF010000000
VERSION     DAORLF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21763_length_2583_cov_1.753165"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK198_26095"
     CDS             complement(<1..318)
                     /locus_tag="PK198_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42300.1"
                     /translation="MNTFFTFELRSWLRSPMPWIFMLLIALLTFGATVSDQISIGGVY
                     GNVWKNSPYVAQNWYAVFSVLALLLVTSFLNSAAIRDFESKTDQIVFSKPVSKAGYYF
                     GHFF"
     gene            complement(434..1306)
                     /locus_tag="PK198_26100"
     CDS             complement(434..1306)
                     /locus_tag="PK198_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF42301.1"
                     /translation="MQLTIRNLSKTYSNGVKALQDVSLDIPTGMYGLLGPNGAGKSTL
                     MRTVATLQTADSGSVMLGDLNVLEDKEELRRRLGYLPQEFGVYARLSAVGMLTHLATL
                     KGIPSGERKEVVNALLQRVNLWEHRKKAVSSYSGGMRQRFGIAQALLGAPQLIIVDEP
                     TAGLDPGERNRFYNLLSEIGENVIVILSTHIVEDVKELCSNMAIINNGRVLYAGSPDA
                     ALEQLQGKVWEKAIAKSEFEAYQQQYQVISSKLVAGKPIIHVLSDARPAEGFQPVDAD
                     LEDVFFAKIMAGSL"
     gene            1465..1788
                     /locus_tag="PK198_26105"
     CDS             1465..1788
                     /locus_tag="PK198_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42302.1"
                     /translation="MKNIVLLSMLASFLCLATSCEKPKLETDSAMLTGTDARRCASPY
                     CGGWFIEINGETKRFLEIPAQTDIDLNGELEFPIPVEVEWKRYDNWWGDIEDLIKVEK
                     LYRED"
     gene            complement(1875..>2126)
                     /locus_tag="PK198_26110"
     CDS             complement(1875..>2126)
                     /locus_tag="PK198_26110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42303.1"
                     /translation="KKDNMGNVWMWGPIPKGHEERYAFRFEYVGDGRYYIKHVYSGKY
                     LCSGDKDNHGRFWLFGPIPKGHEVRYTFDLIRIIGFSSL"
     assembly_gap    2127..2162
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2163..2474)
                     /locus_tag="PK198_26115"
     CDS             complement(<2163..2474)
                     /locus_tag="PK198_26115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42304.1"
                     /translation="MKNSSLYFNSRINMAVQVMAQYLFFVMAFLTMLLCLVHIPIVSK
                     AQTPINNEEVFLRHLYSGKYLCSDKKSNGENFWMWGPIPDGHEQRYIFITQSAGNGKY
                     YL"
BASE COUNT          652 a          601 c          656 g          638 t
ORIGIN      
        1 gaaaaagtgg ccgaagtaat aaccggcttt gctcacaggt ttggaaaaaa cgatctggtc
       61 ggttttggat tcaaaatccc ggatggccgc gctgttcaaa aacgaagtga ccaacagcag
      121 agccagcacc gaaaacacgg cgtaccagtt ttgggccaca taaggcgagt ttttccagac
      181 gttgccgtag acaccgccga tgctgatctg atcggacacg gtggcgccaa aagtgagcaa
      241 cgcaatgagc aacataaaaa tccagggcat cggagagcga agccaagagc gaagttcgaa
      301 ggtgaagaag gtgttcatgt tatgcttttg tggtatgaac catcattttg atatggaaaa
      361 acctctgcgc gaggtccgag cctgtcgttt tactcaatat actgagtaag tttactcaat
      421 gaattgagca aaactataag ctgcccgcca tgatttttgc aaaaaacaca tcctccagat
      481 cggcatccac cggctgaaaa ccctcggcgg ggcgggcatc gctcaggacg tggatgatgg
      541 gtttgccggc caccaacttg ctggaaatga cctgatattg ttgctggtat gcttcaaatt
      601 ccgatttggc gatggctttt tcccacacct tgccttgcaa ttgctccagc gcggcgtccg
      661 gcgagccggc gtacagcact cgcccgttgt tgatgatggc catattggaa cacaactcct
      721 tgacatcctc cacgatgtgc gtgctgagaa tgacgatgac gttttcaccg atttccgaaa
      781 ggaggttgta aaagcggttg cgctcgccgg gatcgaggcc ggctgtgggt tcgtccacga
      841 tgatgagttg cggagcgccc aacagtgcct gcgctatgcc gaatcgctgc cgcataccgc
      901 cggagtacga ggacacggcc ttctttctgt gttcccacaa attgacgcgc tgcagcaggg
      961 cattcaccac ctccttgcgt tcgccggacg gaatgccttt gagcgtggcc aagtgggtca
     1021 gcattcctac ggcgctgagg cgggcataca cgccaaactc ctgcggcagg taaccaagac
     1081 gacggcggag ttcttctttg tcttccagca cattcaggtc gccgagcatg acgctgccgc
     1141 tgtcggcggt ttgcagggtg gccacggtgc gcatcagggt gcttttgccg gcgccgttgg
     1201 gacccagcag gccgtacatg ccggtgggaa tgtctaatga aacgtcttgc agggctttga
     1261 cgccgttgga ataggttttg gaaaggttgc gaatggtcag ttgcatggtt gggtgctttg
     1321 atgttcggca gattttataa gcggctcaaa agtaaataca atgccacgaa tacaacttgc
     1381 ttttagacca aagtttagtt ctgtagggat gtatgcccaa ccatttccta cttatttagc
     1441 gtctcatggg gaaaatctga agccatgaaa aatatcgttt tgttgtccat gctggccagt
     1501 ttcttatgcc ttgccacttc ctgtgaaaag cccaaattag aaaccgacag cgccatgttg
     1561 accggcaccg atgcgcgcag atgtgccagc ccctattgcg gcggctggtt catagaaata
     1621 aatggggaga ctaagcggtt tttggaaatc ccggctcaaa cggatattga cctcaatggt
     1681 gaattggaat ttcccatacc cgtagaagta gaatggaaga ggtacgacaa ctggtggggc
     1741 gatattgaag acctcatcaa ggtggaaaag ctgtacagag aggattgatt ggaaactcct
     1801 cttgaattat ttccaggctg cccaaaccac aagcccgaaa ccaccttgac ggcggcttca
     1861 tatcgccgtg tacactacaa ggaagaaaag cctataatac ggataaggtc aaaggtatat
     1921 ctcacttcat ggcctttggg gataggtcca aacagccaaa accggccatg attgtctttg
     1981 tctccggagc aaaggtattt gccggaatag acatgtttga tgtagtagcg accatctcca
     2041 acgtactcga aacggaaggc atagcgttct tcgtgccctt tcggaatagg cccccacatc
     2101 cagacgttgc ccatattgtc ttttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nncagataat atttaccatt gcccgcagat tgagtaatga aaatatatcg ttgttcgtgt
     2221 ccatcgggaa tcggccccca catccagaag ttctctccat tggatttttt gtcagagcat
     2281 agatacttgc ccgagtagag gtgccttaga aaaacctctt cattgttgat gggcgtctga
     2341 gccttagata caattggaat atgtaccaag cataacagca tggttaagaa tgccattaca
     2401 aaaaagagat actgagccat cacttgtact gccatattga tgcggctgtt aaaatacagg
     2461 cttgaatttt tcatggctga gaatttatgg tgaagaaata gtaaggtcat aaagagatgt
     2521 gaaaaatttt gaataactcg ccacatctcc ctcttccttt ctttttattt aggttgggtc
     2581 act
//
LOCUS       DAORLF010000570         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22141_length_2547_cov_1.618379, whole genome shotgun sequence.
ACCESSION   DAORLF010000570 DAORLF010000000
VERSION     DAORLF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22141_length_2547_cov_1.618379"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>209
                     /locus_tag="PK198_26120"
     CDS             <1..>209
                     /locus_tag="PK198_26120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF42305.1"
                     /translation="FATVSAATLSVLVGGLEPLTLYSFEVAAFDMAGNVSEIAEITLS
                     TTAPLDTGEPGLVAHYPFDGNANDA"
     assembly_gap    210..474
                     /estimated_length=265
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <475..>866
                     /locus_tag="PK198_26125"
     CDS             <475..>866
                     /locus_tag="PK198_26125"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42306.1"
                     /translation="MVWTTNSNNAQFTNFISDMDSGDGNEMVLGFWWYVTMVHDGQFD
                     IIYVNGQEVNRKPVICKLNSTGRPLCFGNNPIEGGQYFIGALDNVKIYNKALTASEIS
                     ALFATGTSSAKEPDFAAAYIESAFPNPAT"
     assembly_gap    867..905
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <906..1092
                     /locus_tag="PK198_26130"
     CDS             <906..1092
                     /locus_tag="PK198_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42307.1"
                     /translation="QDLLLRVFDLTGRQLDAVQIGRAEVASGQFQLDVRKYPAGSYSL
                     NFILGGKNIGSLKFNKQ"
     gene            1483..2238
                     /locus_tag="PK198_26135"
                     /pseudo
     CDS             1483..2238
                     /locus_tag="PK198_26135"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
     assembly_gap    2354..2394
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          495 a          604 c          598 g          505 t
ORIGIN      
        1 tctttgccac tgtttctgct gccacacttt ccgtattagt gggtggtctg gagccgctta
       61 cgctgtattc gtttgaagtg gcggcattcg acatggccgg caatgtttcc gaaattgccg
      121 aaatcacgct cagcaccacc gccccgctcg ataccggcga acccggtttg gtggcccatt
      181 atcccttcga cggcaatgcc aacgatgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggtg
      481 tggacaacca acagcaacaa tgcgcaattc accaacttca tctccgacat ggacagcggc
      541 gacggcaacg aaatggtgct cggcttttgg tggtacgtca cgatggtaca cgacggacag
      601 tttgacatca tctacgtcaa tggtcaggaa gtgaaccgca aaccggtcat ctgcaagctc
      661 aacagcaccg gcaggccctt gtgtttcggc aacaacccga ttgaaggcgg ccagtatttc
      721 atcggcgctt tggacaatgt gaaaatttac aacaaagcgc tcaccgcatc cgaaatatcg
      781 gccctgtttg ccaccggtac cagcagcgcc aaagaacccg attttgcagc agcttatatt
      841 gaatcggcat ttcccaatcc cgcaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnccaag acctcctgct cagagttttt gatctgacgg gccgccagtt ggatgccgtt
      961 cagatagggc gcgcagaggt ggcttcgggc caattccaac tcgatgttcg gaagtacccg
     1021 gccggctctt attcgctcaa ttttattttg ggaggtaaaa acatcggctc gttgaaattc
     1081 aataagcaat aaccgatgtc cctgatctgc aggcgcccca ttcctaaggg acgcttgcag
     1141 atcaggacct ctttttattt aacagccaat cagtcagtct ctcaggtcat ggtcgaacgt
     1201 tttcctcaaa atcctatttt gacaaaagca gacatacggc ccagccatcc cgactgggtg
     1261 gtggaatgtg tgctcaatcc gggggttttt cgcttcgatg gtaaaatctg gctcttgttg
     1321 cgcatcgctg agcgccctgt tcagcgggcc ggcatggtct catttccggt gatgaacggc
     1381 gacggtaaat tccatatcat ggagttttcg ctcaaagact ccctgctcga tttgtcggac
     1441 tcccgaatgg tgggctacaa cggcgccacc tacctctcca ccatgtcgca tctgcgtttg
     1501 gtgtgcagcg aggacggcgt gcattttcgg gaaccggaag acttgcctgc acaaattttt
     1561 gggcagggag cgctcgaaac cctcggcata gaagactgcc gggtgagttt tatagacggc
     1621 gcctaccacc tgacttatac gcaggtgtcg gaaaacgggg tgggggtagg attgatgcgc
     1681 acccaagact ggcggaattt cagccgggac ggcatgattt tgccgccgca caacaaagac
     1741 tgcgccctgt ttgaagaaaa aataggcggg cgatattatt gactgcacag gccctccggc
     1801 ctcggtcccg gcggcaattt catctggatg gccgcttcgc ccgatctcca gcactggggg
     1861 cggcatcagt gcatcctcaa tacccgcgaa aagtggtggg acagcgttcg cgtgggcgcc
     1921 ggggcatcgc cgatcaaaac ttcggaaggc tggttggaga tttatcatgg cgccgatcac
     1981 cagcaccggt attgtctcgg cgcggtgctg ctcgatctgc acgatccatc caaagtactg
     2041 gcccgctcca aagacccgct gctggaaccg gtttatgact acgagcaaag cggctttttc
     2101 ggcaatgtgg ttttcaccaa cggccatatt gtggacggcg acgccattac gatttattac
     2161 ggcgcttcgg atgaggtgat ctgcggcgcg acgctctcgg tagaaggggt gttggcggcg
     2221 ctgctgaggg gggattagtc caccaccttt ccgctttcga ggtcaaaaaa gtgcatttta
     2281 tccaggcggg aaccggcatt acagctgtca gccttgggct gtacatcgcc caaaaacgtc
     2341 acgccttttt cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacacg
     2401 ctacgttttt cggcacttgc aatcggcagt ttcggccgta tgaatgcgtt caggctgtag
     2461 cccaaaaaca cggcaaaccc aagagcaacg cggggaccgg cattacagat tttaggagat
     2521 gggcgacaga aaaaccggaa agaaccg
//
LOCUS       DAORLF010000571         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22151_length_2546_cov_1.825371, whole genome shotgun sequence.
ACCESSION   DAORLF010000571 DAORLF010000000
VERSION     DAORLF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22151_length_2546_cov_1.825371"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>219)
                     /locus_tag="PK198_26140"
     CDS             complement(<1..>219)
                     /locus_tag="PK198_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRF42308.1"
                     /translation="TTQQLGSPGNLMFVVGATHPEQFAEIRQAAPEHFLLVPGVGAQG
                     GDLAEVCRYGMTEEVGLLVNASRAILYA"
     assembly_gap    220..390
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<391..741)
                     /gene="pyrF"
                     /locus_tag="PK198_26145"
     CDS             complement(<391..741)
                     /gene="pyrF"
                     /locus_tag="PK198_26145"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRF42309.1"
                     /translation="MTRQELIGQIRSKQSFLCIGLDPDIDKLPPHLLELDDPVFEFNK
                     AIIEATHDLCVAYKPNLAFFEALGPNGWHTLARTMDCVPPGIFTIADAKRGDIGNTSR
                     LYAKAFFEKYSFDAV"
     gene            816..2192
                     /locus_tag="PK198_26150"
     CDS             816..2192
                     /locus_tag="PK198_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HRF42310.1"
                     /translation="MTVSSNQRSAFAVITALFFMWGFITCLNDILIPHLKKAFDLQYW
                     QAMLVQFAFFGAYFVVSLLYYIYSFASGDPIARAGYQKGLVAGLVLSGIGCALFFPAA
                     GVHSYALFLLALFVLASGITIIQIAANPYVAILGPSETASGRLNLAQGLNSLGYVIAP
                     VIGGAVIFGSTLQGAESVKMPYVALAGAFFVLALIFSFIRLPKVNAGEQMVRENVLVK
                     YPGFAFGMLAIFCYVGAEVAVGSVLVNYLGLKEVMGYDHETANKFLSFYWGGLMIGRF
                     MGAISLGNMKSNTNKALLMAVAAVAGTLVIFAASSRDWFTGVQKLQFSEITLFLIMVA
                     LSYGAFFLGRSKPGRMVGIFSLVIIGLLLLTMGSHGDTAMWTIIGIGLFNSVMWSNIF
                     TLSIEKLGKDTSQGSSLLVMMIVGGALLPPLQGLVADYVTGLKFSLVVPLVAYFYLVW
                     YGFRGWKV"
     gene            2268..>2546
                     /locus_tag="PK198_26155"
     CDS             2268..>2546
                     /locus_tag="PK198_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42311.1"
                     /translation="MTNAIKLLFGMLLAAVAVNAQTEHICKSTDEAAQAAHTRLLEFR
                     SSPQTQNYDVKYHRFEWVINPTVRYISGVVTTYFTAREEGFQTLHFDFD"
BASE COUNT          487 a          663 c          621 g          604 t
ORIGIN      
        1 cggcatacaa aatggcccgc gaggcattca ccaacagccc cacctcctcg gtcatgccat
       61 agcggcacac ctccgccagg tcgccgccct gcgcgcctac gcccggcacc aacaaaaaat
      121 gctccggcgc tgcttgtctg atctctgcaa attgctccgg gtgcgtcgcc cccaccacaa
      181 acatcagatt gccggggctg cccaattgct gcgtagttcn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taccgcatca aagctgtatt tttcaaaaaa
      421 agccttcgca tacagccgcg aggtattgcc gatgtcgccg cgcttggcat ccgcaatggt
      481 gaaaatgccc ggcggcacac agtccatggt tcgggccagc gtatgccagc cgtttggacc
      541 cagggcttca aaaaaagcga gattgggttt gtaggccaca cagagatcgt gcgttgcctc
      601 aatgatggct ttattaaatt caaaaaccgg gtcgtccaac tccaacaggt ggggcggcag
      661 tttgtctatg tctggatcaa ggccgatgca gaggaaagat tgcttgctgc ggatttgccc
      721 gatgagttcc tggcgcgtca tactcttttt ttcgcgaagg tagtcaccaa cccatgaaca
      781 tggttaactt cgcccatcaa aatcagcacc aaccaatgac cgtatcatcc aaccaacgca
      841 gcgcatttgc cgtcatcacg gctttgtttt ttatgtgggg cttcatcacc tgcctcaacg
      901 acatcctcat tccgcacctc aaaaaagcct tcgatttgca atactggcag gccatgctgg
      961 tccaatttgc atttttcggg gcctattttg tggtgtctct gctgtactat atttattctt
     1021 tcgcatcggg cgaccccatc gcgcgggcag gctatcagaa aggactcgtg gcaggcctcg
     1081 tgctcagcgg tataggctgc gccctgtttt ttccggccgc cggcgtgcat agctacgcgc
     1141 tgttcctgtt ggcgctcttt gtactggcct ccggcatcac catcatacaa atagcggcca
     1201 atccctatgt ggccattctc ggcccgtccg aaacggcctc cggcaggctc aatctggccc
     1261 agggactcaa ctctttgggc tacgtcattg cgcccgtcat cggcggtgcg gtcattttcg
     1321 gcagcacgct tcagggcgcc gaatcggtaa aaatgcctta tgtggcattg gccggtgcgt
     1381 tttttgtttt ggccctgatt ttcagcttta tacgcctgcc caaagtgaac gccggagagc
     1441 aaatggtgcg ggaaaacgtg ttggtcaaat accccggttt cgctttcggc atgttggcca
     1501 ttttctgtta tgtaggtgct gaggtggcag tcggcagcgt gttggtcaac tacctcggcc
     1561 tcaaagaagt gatgggctac gaccacgaaa cggccaacaa atttctctcc ttttactggg
     1621 gcggcctcat gatcgggcgc ttcatggggg ctatttcgtt gggcaatatg aaaagcaaca
     1681 ccaacaaagc cctgctcatg gcggtggcag cggtggcggg cactttggtc atttttgcgg
     1741 cttcttcgcg cgactggttc acgggcgtgc agaaattgca gttcagcgag attaccttgt
     1801 ttttaatcat ggtagcgctc agctacggcg cattttttct ggggcgttcc aaacccggtc
     1861 gcatggtggg tattttttcg ctggtcatca tcggcctgtt gttgctcacc atgggcagcc
     1921 acggcgacac ggccatgtgg accatcatcg gcatcggctt gttcaactcg gtcatgtggt
     1981 ccaatatctt cacgctttcc attgaaaaat tgggcaaaga caccagtcag ggcagctctc
     2041 tgttggtgat gatgattgtg ggcggggctt tgttgccgcc gctccaaggc ttggtcgccg
     2101 actatgttac cggactgaaa ttctcgctcg tggtgccctt ggtggcgtat ttctatttgg
     2161 tgtggtatgg atttcggggg tggaaggtat agggcagtga tatttctcac ccaacgcagc
     2221 ttggattttc cggtatcttt gcgcgcacaa atttgaagtc gaaaataatg accaacgcta
     2281 tcaaacttct attcggcatg ctgctggcag ccgtcgccgt caatgctcaa acagagcata
     2341 tttgcaaatc caccgacgaa gccgcacagg ccgcccacac gcggctttta gagtttcgca
     2401 gttcgccgca aacccaaaac tacgacgtaa aataccaccg tttcgagtgg gtcatcaacc
     2461 ccacggtgcg ctacatcagc ggcgtcgtca ccacttattt tactgcccgc gaggagggtt
     2521 ttcaaaccct gcatttcgac tttgat
//
LOCUS       DAORLF010000572         2540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22207_length_2540_cov_1.606036, whole genome shotgun sequence.
ACCESSION   DAORLF010000572 DAORLF010000000
VERSION     DAORLF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22207_length_2540_cov_1.606036"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>304
                     /locus_tag="PK198_26160"
     CDS             <1..>304
                     /locus_tag="PK198_26160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF42312.1"
                     /translation="AHLAFSCPKEEPDIVVPTEPQPQQYGTPFDKVPDIADVQMYEVN
                     PRVFSPQKNLDGITARLDSLRQLGVNVVWLMPIYPTGGERSVGAPYAIKDYPSGHPD"
     assembly_gap    305..332
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <333..1334
                     /locus_tag="PK198_26165"
     CDS             <333..1334
                     /locus_tag="PK198_26165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF42313.1"
                     /translation="LVDKAHALDMAVMLDWVANHTAWDHAWIADKTWYVTDGSGNIVH
                     PPGTNWQDVAELNYANSKMRLEMIKAMKYWVLEANVDGFRCDYATGVSSKFWKQAIDT
                     LRNIPNRDLILFAEAEDKALLNAGFDLIFGWPYYGRLKDVFSGLAATQIHNMHLTEYN
                     GLASGKHVVRWITNHDQHAWEATPQSIFGSVSAATAAFVITSYMGGVPLIYNGQEVAT
                     PNQLPFFEGTNATINWNLNPAIKAEHERILNLRKNSAALRRGTLTDYSSSNVAAFTRK
                     QGNEEVLVLVNVRNSNQDFTLPANLRLSDWYDAYSNAPVSLATTVSLGAYQYRVLKR"
     gene            1518..>2104
                     /locus_tag="PK198_26170"
     CDS             1518..>2104
                     /locus_tag="PK198_26170"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42314.1"
                     /translation="MKQLIFTFLLFLGGLAFSLNASAQERARLETAQNHIKANLEKWQ
                     LSAADVADLVVSDNYRSDHNGVHHFYFIQRHAGIEVHTAIMGVHVKGDKVAYAVHRFV
                     PELASKINTTQPLITSRQAIDFAAKQVGAYTPTTLRQIGHPQEHLYVYEGGAMAKSDI
                     TVKLVFQQMRKSGAVRLAWHVTLDQMDTSDWWSIRV"
     assembly_gap    2105..2136
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2137..>2540
                     /locus_tag="PK198_26175"
     CDS             <2137..>2540
                     /locus_tag="PK198_26175"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF42315.1"
                     /translation="KNNWTTYCNFRHDAGHQHSADCVGHAAESAQTTVDNLLLNDGAQ
                     YKVFPLPAESPIHGTHQLVINPADPIASPFGWHDINGVAGPEFTTTRGNNVRAYLDTL
                     GTNQLSEGPSGGAALVFDFPHDANLEPAQMRP"
BASE COUNT          617 a          697 c          614 g          552 t
ORIGIN      
        1 gctcatctgg ccttttcctg tccaaaagaa gaacccgaca tcgtcgttcc cacagaaccg
       61 cagccacagc agtacggaac gcccttcgac aaagtacctg acatagcaga tgtgcagatg
      121 tacgaagtca atccacgggt tttcagtcct caaaaaaact tagacggcat caccgcccgt
      181 ttggactcgc tccgacaatt gggcgtcaac gtggtatggc ttatgcccat ttatcccact
      241 ggcggggagc gcagcgtggg ggcgccttat gccataaaag actacccgtc gggtcatccc
      301 gactnnnnnn nnnnnnnnnn nnnnnnnnnn nnttggtgga caaagcccat gcgctcgata
      361 tggccgttat gctggactgg gtggccaacc atacggcctg ggatcatgcc tggatagctg
      421 ataaaacctg gtatgtgacc gacggcagcg gcaatattgt gcatccgccc ggcaccaact
      481 ggcaggatgt ggccgagctg aattatgcca actccaaaat gcggctcgaa atgataaagg
      541 ccatgaaata ctgggtactc gaagccaatg tggacggttt tcgctgcgat tatgcgacgg
      601 gagtcagctc caaattttgg aaacaagcta ttgatacgct ccgcaacatt cccaacagag
      661 acctcatcct gtttgcggaa gctgaagaca aggccctctt gaacgcaggt ttcgacctca
      721 tttttgggtg gccgtattac gggcggctca aggatgtgtt cagcggactg gctgccacac
      781 aaattcacaa catgcacctc accgagtaca atggcctagc ctccggcaag cacgtcgttc
      841 gctggatcac caaccacgat cagcacgcct gggaggccac tcctcagagc attttcggca
      901 gtgtgagtgc tgctacggcg gcctttgtca taacgagcta catgggcggc gtgcctttga
      961 tttataatgg tcaggaagtt gccaccccca atcagttgcc tttttttgaa ggcacgaacg
     1021 ccaccatcaa ttggaatctc aacccggcaa taaaagctga acatgagcgc attctgaatt
     1081 tgagaaaaaa cagcgccgca ctgcgccggg gcacgctgac ggactacagc agcagcaatg
     1141 tggccgcatt tactcgaaaa caaggcaacg aagaggtatt agtgctggtg aatgtgcgca
     1201 attccaatca ggatttcacc ctgcccgcca accttcgcct cagcgattgg tacgatgcgt
     1261 acagcaatgc gccggtttca ttagcgacta ccgtttcatt gggggcttat cagtacaggg
     1321 tgttgaaaag gtgatttaat gcagcttttg gcttatttgt cggctgccat atatattttc
     1381 ctccgaacgc gctgccggaa agtccttacc gaccaataca tttgccccgc ccaatggcaa
     1441 acaatatggc cgtccaggca tttattgaac tgttattttg ctttttccaa aacgtctata
     1501 aatttcatcc aaacgacatg aagcaactca tctttacatt tctgttgttt ctcggcggct
     1561 tggcgttctc tttgaacgca agtgctcagg aacgcgctcg gctcgaaacg gcgcaaaacc
     1621 acatcaaggc caatcttgaa aaatggcaac tcagcgctgc cgatgtggct gacctggttg
     1681 tgagcgacaa ctaccgctcc gatcacaatg gcgtacatca tttttatttc attcaacgcc
     1741 acgcaggcat tgaggtgcat accgccatca tgggcgtgca tgtgaaggga gataaggtgg
     1801 cctatgcggt gcatcgtttc gtgcccgaat tagcctccaa aatcaatact acgcagccgc
     1861 tcattacctc gcgtcaggcc attgattttg cagccaaaca agttggagct tacacgccga
     1921 ccaccctgcg ccaaatcggg catccgcaag agcatttgta tgtgtatgag ggcggcgcta
     1981 tggccaagtc cgacatcacc gtcaaattgg ttttccaaca aatgcgcaaa tccggtgcgg
     2041 tgcgtttggc atggcatgtc accctcgacc aaatggatac ctccgactgg tggagcatcc
     2101 gtgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa aacaactgga caacctattg
     2161 taatttccgg catgacgcag gccaccagca cagcgccgac tgcgtaggac acgcggcgga
     2221 aagcgcccaa accaccgttg acaacctgct gctgaacgac ggcgctcaat acaaggtgtt
     2281 tcccttgccg gcagaaagcc ccatacacgg cacgcatcaa ttagtcatca accctgccga
     2341 tccgattgca tctcctttcg gctggcacga catcaacggc gtagcagggc cggaatttac
     2401 caccactcgt ggcaacaatg ttcgagccta cttagatacg ctcggcacca atcaactcag
     2461 cgaaggcccc agcggcggcg cagctttggt attcgacttt ccacacgacg caaaccttga
     2521 acccgcacaa atgcgtccgg
//
LOCUS       DAORLF010000573         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22269_length_2535_cov_1.875000, whole genome shotgun sequence.
ACCESSION   DAORLF010000573 DAORLF010000000
VERSION     DAORLF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22269_length_2535_cov_1.875000"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>783
                     /locus_tag="PK198_26180"
     CDS             <1..>783
                     /locus_tag="PK198_26180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42316.1"
                     /translation="SCLAADNITTYNRYFDEAEKAVADQPRLLLHVREARMPLQYAMM
                     EIGKSDMFGPRGWYDERNGDFVPRRNMLDMLEQFYQTGRDIRSEPVNESGLSVEAYYQ
                     STRRFIEVQVQGNLAFRKPVSADPPPAEKYSGGDLAVLSNGVRGANDFKVHWLGWESK
                     NFTLNLDLQQLRQAETIEISTLWDPKSWILHPRSIACSVSEDGLGFEQVGAIVAAGDQ
                     RQEEVNRLFAFQTGGRAFRFVRFEVLGTLRLFDWHPSAGGGS"
     assembly_gap    784..825
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            977..>1699
                     /locus_tag="PK198_26185"
     CDS             977..>1699
                     /locus_tag="PK198_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF42317.1"
                     /translation="MKVEQIYTGCLAEAAYYIESNGEAAIVDPLRDVEPYLERAKADN
                     AKIKFVLETHFHADFVSGHLDLAKQTGATIVYGPTAQPNFQAHTAADNEEFKVGNVTI
                     KVLHTPGHTMESSTFLLIDENGKDYAAFTGDTLFLGDVGRPDLAQKAGSITQEDLAGW
                     LFDSLRNRIMPLADDVIVYPGHGAGSACGKKMAAETTGTLGEQKRSNYALRADMTRDE
                     FIKEVTDGLAPPPQYFPKNAVMN"
     assembly_gap    1700..1714
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1715..2386
                     /locus_tag="PK198_26190"
     CDS             <1715..2386
                     /locus_tag="PK198_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF42318.1"
                     /translation="DNVRAKGMRPLSVRAFKAAWEGEGAMVLDTRVQDHFAEGFIPGS
                     IFVGIDDAFAPWVGALVEDLAVPILYVCEPGREQEVVDRLARVGYDNPIGFLEGGFPA
                     WVAAGEEVDSIKEVYAKEFEGIYNEHAVNILDVRRNSEYRAQHIVGAQNFPLDFINKN
                     MSEVDRHKEYYLHCVGGYRSMIAASILRSRGFDHLVNIRSGFRALQEETKLPVTEFVE
                     QNTEL"
BASE COUNT          581 a          692 c          687 g          518 t
ORIGIN      
        1 cagctgcctc gccgccgaca acatcaccac atacaaccgc tatttcgacg aggcagaaaa
       61 ggccgtagcc gatcagcccc gcctgctgct gcacgttcgc gaagcccgta tgccgctcca
      121 gtacgccatg atggaaatcg gcaaaagcga catgtttggc ccgcgcggtt ggtacgacga
      181 acggaacggt gattttgtgc cgcgtcgcaa tatgctggac atgctggagc agttttacca
      241 aaccggcaga gacatccgct ccgagccggt caacgaatcg ggcctcagcg tggaggctta
      301 ttaccagtcc acccggcgct tcatcgaagt gcaggtgcag ggcaacctcg ccttccgcaa
      361 acccgtgagc gccgacccgc cgcccgcaga aaaatacagc ggcggcgatc tggctgtact
      421 gagcaacggc gtgcgcggcg ccaacgactt caaagtacac tggttgggct gggaatcgaa
      481 aaacttcacg ctgaacctcg atttgcaaca actccggcag gccgaaacga tcgaaatcag
      541 cactttgtgg gaccccaaaa gctggatttt gcacccccgc tccattgcgt gcagcgtatc
      601 ggaagacggg ctgggcttcg agcaggtggg tgctattgtg gcagcaggcg atcaacggca
      661 ggaggaggtc aaccggctgt ttgcctttca gaccggcggg cgggcattcc gttttgtgcg
      721 tttcgaggtg ttgggcacgc tgcgcctttt cgactggcac ccctcggcgg gcggcggctc
      781 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgagg aaaaaaaatc
      841 atcatagtga catatatcac ttacaccccc aacggcaggc tataattttg cacccgtatt
      901 caagcggaat attttcgctg caaatacaaa agagagttca tataattttt cctaaccgtt
      961 caaaacgttt tttcctatga aagtagaaca gatctatacg ggttgccttg ccgaagccgc
     1021 ttactacatc gagagcaatg gcgaagctgc tattgtggac ccactgcgcg atgtggaacc
     1081 gtatttagaa agggccaagg ccgacaatgc gaagattaag ttcgtgctcg aaacccattt
     1141 tcatgccgat tttgtatcgg ggcacttaga cttagccaag caaaccggag ccaccatcgt
     1201 gtatggccct accgctcagc ccaattttca ggcgcatacc gcagccgaca acgaagaatt
     1261 caaagtgggc aatgtcacca tcaaggtatt gcacacgccc ggccacacta tggaatcttc
     1321 caccttcctg ctcatagatg aaaacggcaa agactacgcc gctttcactg gcgatacgct
     1381 gttcctcggc gatgtaggcc gtcccgacct ggctcagaaa gccggttcca tcacgcagga
     1441 agacctcgca ggttggttgt tcgacagcct ccgcaatcgc atcatgcctt tggccgacga
     1501 cgttatcgtg tatcccggcc acggtgcggg ttctgcctgc ggcaaaaaaa tggccgccga
     1561 aaccaccggc accctcggcg aacaaaaacg cagcaactac gctctccgcg ccgatatgac
     1621 ccgcgatgaa ttcatcaaag aagtcaccga cggattggcg ccgccgccgc agtatttccc
     1681 caaaaatgcg gtgatgaatn nnnnnnnnnn nnnngacaac gtgcgcgcca aaggtatgcg
     1741 tccgctcagt gtgcgcgctt tcaaagcagc ctgggaaggc gaaggtgcta tggtgctcga
     1801 tacccgcgtg caggatcact ttgctgaagg cttcattccc ggctccattt ttgtaggtat
     1861 tgacgacgca tttgctccct gggtaggcgc tttggtagaa gacctcgccg tgcccatcct
     1921 ttatgtgtgc gaacccggcc gcgaacagga agtggtggac cgtctggccc gcgtgggtta
     1981 tgacaatccc ataggtttcc tcgaaggtgg tttcccagcc tgggtagctg ccggcgaaga
     2041 agtggacagc atcaaagaag tgtacgccaa ggagtttgag ggcatctata acgaacatgc
     2101 cgtgaacatc ctcgacgtgc gccgcaacag cgagtacaga gcgcagcaca tcgtgggcgc
     2161 gcagaacttc ccgcttgatt tcatcaacaa gaacatgagc gaagtggatc gccacaagga
     2221 gtactacctg cactgcgtgg gcggctatcg ctccatgatt gcagcttcga tcttgcgcag
     2281 ccgcggtttc gatcacttgg tgaacattcg cagtgggttc cgcgcgttgc aggaagagac
     2341 caagctgccg gtgacggagt ttgtggaaca gaacacagag ttgtagtttt ttcgatagct
     2401 cagaatgagg gctatccggt aataataagt tgataacagg gcttcgggcc gccggttttt
     2461 aaatcggcag tccgaagcct tttgtgtgtg ccccgaatgg gcatcgaaga agaccagtcg
     2521 gcggctatcg gtaca
//
LOCUS       DAORLF010000574         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22270_length_2535_cov_1.851613, whole genome shotgun sequence.
ACCESSION   DAORLF010000574 DAORLF010000000
VERSION     DAORLF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22270_length_2535_cov_1.851613"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK198_26195"
     CDS             complement(<1..402)
                     /locus_tag="PK198_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42319.1"
                     /translation="MIGWKFSAYTPDEEGSTFDRLFKLFQELLIHTSGSVSEALAWLT
                     ELDKEYNLTDDNYGMADFIQDLIDKGYIQQEGGVEGFPGFVPSSKLELALRRKALEDV
                     FGQLKKSKSGNHNTRRYGRGDEHTSELRPFEF"
     gene            complement(801..>1103)
                     /locus_tag="PK198_26200"
     CDS             complement(801..>1103)
                     /locus_tag="PK198_26200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42320.1"
                     /translation="GAALWRGGLVSLTPRFEKRWLELAVPVSLYHGNRLQVGVAGRIG
                     GLTIGSDRIGSVLGRTNWSGSDIYAALKIQPFKIQTTGKKSGKGRRGNGDVKCYKF"
     assembly_gap    1104..1113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1114..2256)
                     /locus_tag="PK198_26205"
     CDS             complement(<1114..2256)
                     /locus_tag="PK198_26205"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRF42321.1"
                     /translation="MKLVATFSIAFIFSFSQLCAQEQIGLRTGNFAGINGWALNPSSH
                     TTTPFNWELNLLEGAFFFDNNYAFLSKTGLSHLTANRDNIEFAFGPDYDKETPPPSGS
                     VVLDFYTNARKRYAQGMSSALGPSLFVRIGPNHTIGAFVRGRAFASARGLVNDFSYYD
                     YFNRPFEVPFEVTPFSVSMAAWSEYGLNYLFRTETATGTLGIGINARLLQAYEGIYLH
                     SQTAFPLAKLPGDSLSGGPIVFEYGHTRTNLSADEPAIRPNGGGAAFDIGLTYILGEE
                     TDYTWRFGFSILDIGRLQFNRNARLHRAAPDASSIIGFDNYNDFVMPGDYENMLARFS
                     SDLLGNPDASFTGDRFAMWLPAAVSVQIDRNLGAGFFLNAVVVQGIP"
     gene            complement(2368..>2535)
                     /locus_tag="PK198_26210"
     CDS             complement(2368..>2535)
                     /locus_tag="PK198_26210"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRF42322.1"
                     /translation="NTALEYLTDLVSPHQHPLAGKAKDAAAAAVMLAAFTAAVVGGII
                     FLPRIFRFLAG"
BASE COUNT          616 a          621 c          708 g          580 t
ORIGIN      
        1 aaactcaaat ggacgtagtt cggaggtgtg ttcatcgccg cggccgtacc tgcgggtgtt
       61 gtggttaccg gatttggatt ttttcaattg gccgaaaacg tcttccaatg ctttgcgacg
      121 aagcgccagt tccaacttgg aggaaggtac aaaacccggg aatccctcta ccccaccctc
      181 ttgttggata tagcccttgt ctatcaagtc ttggatgaaa tcggccattc cataattgtc
      241 gtccgtcagg ttgtactctt tgtccaattc ggtgagccaa gccagcgcct ccgacacact
      301 gcccgatgta tgaatcagca gttcctgaaa caacttgaaa agcctgtcga aggtgcttcc
      361 ttcttcgtcg ggcgtgtagg ccgaaaattt ccatccgatc ataggctgaa ttgttgctcc
      421 cgattcctta cgtcatcgag ttgtttttat acaatgcact gattatcaat aaaaagcatg
      481 atttaaatta tgcgtttcag tcactaataa tgcggatgag cacttgttgg ttcacgacct
      541 tagagggatt ttttgctgta ataacttttt cttgtcagaa atttacgtct gagtaaaaga
      601 tttttatctt tatttttttg cacacattgc aactaccgcg cgtagcaccc gtcgccggca
      661 cacgcgcgtt gcgtgagggg gccgaaggta gtgacgacga aggaggagcg actgaggcgc
      721 ccgacggtgg cgcagcggcc tgccggtact ggggaagagg ccgccggcgc taccggcacg
      781 ccctgaaaat cgttggaatg ctaaaactta tagcacttca catcgccatt gcctctcctg
      841 ccttttccag atttttttcc ggtagtttgg attttgaagg gttgaatttt cagggctgcg
      901 taaatatccg agccgctcca attggttcgc cccaagacgc tgccgatacg atctgagcct
      961 atggtaagcc cgccgatgcg ccccgcaacg cctacctgta agcggttgcc atgatacaat
     1021 gaaacgggga ctgccaactc cagccagcgt ttttcaaacc tcggagtgag cgaaacaagc
     1081 ccaccacgcc aaagcgctgc tccnnnnnnn nnnagggatg ccctgcacca ctacggcatt
     1141 gaggaaaaag cctgccccaa gattgcggtc tatctgcacg ctgacggcag ccggcagcca
     1201 catcgcaaag cgatcacccg tgaaggaagc atcggggttg cccagtaagt cactgctgaa
     1261 acgggccagc atgttttcat aatcgccggg catgacgaaa tcattatagt tgtcgaaacc
     1321 aataatggag gaagcatcag gggcggcccg gtgcaggcgc gcattcctgt tgaactgtag
     1381 cctgccgatg tctaaaatac tgaagccgaa gcgccaggta taatccgtct cctcgccgag
     1441 tatgtaagtg agcccgatgt cgaaggccgc gccgccgccg ttgggtcgaa tggcaggttc
     1501 atcggcactc agattggtgc gggtatggcc gtattcaaac acaatggggc cgccggaaag
     1561 cgaatcgccg gggagcttgg ccaatgggaa ggctgtttgg ctgtgcaaat aaatgccttc
     1621 atacgcctga aggagccgcg cgttgatacc gataccaagc gtgccggtag ccgtttcggt
     1681 gcggaagaga taattgaggc catactcgct ccacgcggcc atgctgacag agaatggcgt
     1741 gacctcgaag ggtacttcaa agggcctgtt gaagtagtcg taatagctaa aatcattgac
     1801 caaaccgcgt gcagaggcga aagcgcggcc gcgtacaaaa gcgccaatag tatgattggg
     1861 gccgatgcgc acaaacagac ttggccccaa ggccgaactc attccctgag cgtagcgttt
     1921 gcgggcattg gtgtaaaaat cgaggacgac gctaccagat ggcggaggcg tttctttatc
     1981 gtagtctggc ccaaaggcga actcgatgtt gtctcgattg gcagtgaggt gagacaggcc
     2041 ggttttggac aaaaaggcgt agttgttgtc gaaaaagaag gcgccctcca gcaggttgag
     2101 ttcccagttg aaaggtgtgg tagtatgaga ggaagggttg agcgcccaac cgttgatgcc
     2161 ggcgaaatta cccgtgcgga gtccgatctg ctcctgtgca caaagctggg aaaaactgaa
     2221 aatgaacgca atagaaaatg tggcgacgag tttcatcaga tagctgcttt cgtttttggg
     2281 aaaaatcagg cgcaagcctt acgaacgatt aacgtgtcaa tatccacatt ttcgtgcaag
     2341 gggcttgggc ttagttagaa aacggattca tccggcgagg aaacggaaaa tgcggggtaa
     2401 aaatataatg cctcccacga ctgcggccgt aaaagcggcg agcatgacag cggcagcggc
     2461 tgcgtctttg gctttgccgg caagcgggtg ctgatggggc gaaactaagt ctgtaaggta
     2521 ttcgagggcg gtatt
//
LOCUS       DAORLF010000575         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22350_length_2527_cov_1.648463, whole genome shotgun sequence.
ACCESSION   DAORLF010000575 DAORLF010000000
VERSION     DAORLF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22350_length_2527_cov_1.648463"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1432
                     /locus_tag="PK198_26215"
     CDS             <1..>1432
                     /locus_tag="PK198_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42323.1"
                     /translation="LTVNQRNPSEIWVGTGEGNPRNSHNSGEGIYKSLDGGKNWKCMG
                     LQATKTIHRIIVHRDNSDVVFAAAMGSIWGPNPERGVYKTSDGGKTWRLVLSVNDRTG
                     CAHLIVDPTNPNKLIAAMWEYGRQPWTFNSGGAGSGIYISHDGGETWEKRTEKDGLPK
                     GHLGRVGLAVAQNKPNVVYAIVEAEENALYRSDDGGFKWRKTTAENIGGRPFYYHDIF
                     VDPKNENRVYSIHTYLNRSEDGGRSFETWIGWTVHLDHHAFWIHPDEPDFLITGNDGL
                     NISRDGGQTWQFADNLPLGQFYHVNYDMDIPYNLGGGMQDNGTFVGPSSVWQVGGIGN
                     HHWQEVHFGDGFDLGFRPDNNRYVYAMSQGGNLSYVDRQTGHTRNIRPVHPDGTALRF
                     NWNAGFAQNPFHNCGVYYGSQFVHKSMDCGQSWQIISPDLTTNDPEKQKQDQSGGLTL
                     DVTAAENHTTILAIAPSPADENALWVG"
     assembly_gap    1433..1442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1443..>2527
                     /locus_tag="PK198_26220"
     CDS             <1443..>2527
                     /locus_tag="PK198_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42324.1"
                     /translation="KGQPHESFSSDGGRTWTNLSSRLPGVKAGSWIPYIEVSQKNAGE
                     AFVAVSDYRRNDWRPMVFHTNDFGATFRRVVDETQVRGHAQAILQDPVEPNLLWLGTD
                     NGLWLSIDGGKNWNQWTNDYPSVQTADLKIHPREHDLIVATFGRALWVLDDIRPIREL
                     ARTQGKVLEQPFRVFEAPDAYLASYKSYDGYHFPADGLYRAPSRPAGALITVWHTTRQ
                     PKMELPAPVSAKGKKTVALPAPTPPAPMPKAPKDGRAKVEVYDEAGLLIRSFSTRIDT
                     GMNRIIWDLRADGMRFPSRQEPRGEAGQLPPGLEVLPGKYKLVFTFGEMKDSTTVTVH
                     GDPRLPISMADRKAKLSAYSEYKTVVE"
BASE COUNT          589 a          789 c          695 g          444 t
ORIGIN      
        1 actgaccgtc aaccagcgca accccagcga gatatgggtg ggcaccggcg agggcaatcc
       61 ccgcaactcc cacaacagcg gcgagggtat ttataaatca ttggacggag gcaaaaactg
      121 gaagtgtatg ggcttgcagg ccaccaaaac catacatcgc atcattgtac accgcgacaa
      181 ctccgatgtg gtgtttgctg cggccatggg ttcgatatgg ggccccaacc cggagcgcgg
      241 cgtgtataaa accagcgacg gcggcaaaac ctggcgcctt gtgttgtcgg tgaacgaccg
      301 caccggctgc gcccacctca ttgtggaccc aacgaatccc aacaagctca tcgctgccat
      361 gtgggaatat ggccgccaac cctggacctt caactcgggc ggcgcggggt cgggcatcta
      421 catcagccac gacggcggcg aaacctggga aaagcggacc gaaaaagacg gtttgcccaa
      481 aggtcatctg ggccgcgtgg ggctggcagt ggctcaaaac aaacccaacg tggtgtatgc
      541 cattgtggaa gcagaggaaa atgccttgta ccgcagcgac gacggcgggt tcaaatggcg
      601 caaaaccacg gcggaaaaca tcggagggcg accgttttat taccacgata tttttgtgga
      661 ccccaaaaac gaaaaccgcg tttatagcat ccacacctac ctgaatcgca gcgaagacgg
      721 cggccgctcc tttgaaacct ggatcggatg gacggtacac ttggaccacc acgccttctg
      781 gattcacccc gacgaaccgg atttcctcat caccggcaac gacggcctca acatatcccg
      841 cgacggcggc caaacatggc aatttgcaga caacctgccc ctgggccagt tttaccacgt
      901 caattatgac atggatatcc cctacaacct cggcggcggt atgcaagaca acggcacctt
      961 tgtggggcct tcctctgtgt ggcaggtggg cggcatcggc aatcaccact ggcaggaagt
     1021 acacttcggc gacggcttcg acctcggctt ccggcccgac aacaatcgct atgtgtatgc
     1081 catgtcgcag ggcggcaacc tcagttatgt agatcgccag accggccaca cgcgcaacat
     1141 acggcccgtg catccggacg gtacagcgtt gcgtttcaac tggaatgcgg gttttgccca
     1201 aaatcctttc cacaactgcg gcgtctatta cggcagccaa tttgtacaca aaagtatgga
     1261 ctgcggccaa agctggcaga tcatatcgcc cgacctgacg accaacgacc ccgaaaagca
     1321 aaagcaagac cagagcggcg gcctcacctt agacgttacc gccgctgaaa accataccac
     1381 cattttggcc attgcgccca gtccggcaga cgaaaacgca ctctgggtgg gcnnnnnnnn
     1441 nngaaagggc agcctcacga gagcttttca agcgacggcg gccgcacctg gaccaacctg
     1501 tcctcccgat tgcccggcgt gaaggccggc agttggattc cctacatcga ggtatcgcaa
     1561 aaaaatgcag gcgaggcatt cgtagcagtc agcgattacc ggcgcaacga ctggcggcct
     1621 atggtgtttc ataccaatga cttcggggcc acgttccgcc gcgttgttga cgaaacgcag
     1681 gtgcgcggac atgcccaagc catcctgcaa gacccggtag aacccaatct cctttggctc
     1741 ggcaccgaca acggcctgtg gctgagcatt gacggcggca aaaactggaa ccaatggacc
     1801 aatgactacc catcggtgca aaccgccgac ctcaaaatac acccccgcga acacgacctc
     1861 attgtggcca cttttggccg cgcactgtgg gtactcgacg acattcgccc catccgcgaa
     1921 ctggcccgca cgcagggcaa agtattggag cagcccttcc gggtgtttga agctcccgat
     1981 gcttatttgg cctcttacaa atcttacgac ggctaccact tcccggcaga cggcctgtac
     2041 cgggcgcctt cccggcccgc cggcgcgctc atcaccgtat ggcacaccac gcggcagccc
     2101 aaaatggaat tgccggcgcc tgtttctgcc aaaggcaaaa agaccgtcgc gctgcctgct
     2161 cccacgccgc ccgcccccat gcccaaagcg cccaaagacg gccgggccaa ggtggaggtg
     2221 tatgacgaag cgggactgct catccgcagc ttcagcaccc gcattgatac cggcatgaac
     2281 cgcattatct gggacttgcg ggccgacggc atgcgtttcc cctcccggca ggagccgcgc
     2341 ggcgaggcgg ggcaattgcc gcccggattg gaggtcttgc ctggtaaata caagctcgtt
     2401 tttacttttg gcgagatgaa ggattcgacc accgtgaccg tgcatggcga cccgcgcctc
     2461 cctatttcga tggccgaccg caaggccaaa ctgagtgcct acagcgagta caaaacagtg
     2521 gtggaaa
//
LOCUS       DAORLF010000576         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22386_length_2524_cov_1.972864, whole genome shotgun sequence.
ACCESSION   DAORLF010000576 DAORLF010000000
VERSION     DAORLF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22386_length_2524_cov_1.972864"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>347)
                     /locus_tag="PK198_26225"
     CDS             complement(<1..>347)
                     /locus_tag="PK198_26225"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF42325.1"
                     /translation="DDEDRRAILDWVEDHAEAIDLIYHQGRIGETYNIGGNNEWKNID
                     LVHALCDVMDELLGRAAGTSRQLIHFVTDRPGHDRRYAIDASKLKNDLGWAPTVYAED
                     GLRRTAQWYLDNA"
     assembly_gap    348..378
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<379..>703)
                     /locus_tag="PK198_26230"
     CDS             complement(<379..>703)
                     /locus_tag="PK198_26230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF42326.1"
                     /translation="FIYTNVLGTVRLLHAARQHWKDNMDGKRFYHVSTDEVYGSLGET
                     GFFTETTPYDPRSPYSASKAASDHFVRAFHHTYGLPVVLSNCSNNYGPCQFPEKLIPL
                     MINNI"
     assembly_gap    704..733
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<734..1006)
                     /locus_tag="PK198_26235"
     CDS             complement(<734..1006)
                     /locus_tag="PK198_26235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF42327.1"
                     /translation="MTILITGGAGFIGSHLVRRMLHRYPQYHIVNLDLLTYAGNLENL
                     RDVENLPNYTFVKGSIEDEAFLDQLFQQYDFQGVIHLAAESHVDRSI"
     gene            complement(1222..2160)
                     /locus_tag="PK198_26240"
     CDS             complement(1222..2160)
                     /locus_tag="PK198_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF42328.1"
                     /translation="MKRVLITGVAGFLGSHLADRFIREGYHVIGMDNLLTGNMDNIAH
                     LFPLKEFEFYFHDVSKFVHVPDNLDYILHFASPASPIDYLKMPIQTLKVGALGTHNLL
                     GLAKAKNARILVASTSEVYGDPLEHPQTEGYWGNVNPVGPRGVYDEAKRFMEAITMAY
                     HTYHGVDTRIVRIFNTYGPRMRVEDGRALPAFFSQAIRGEGLTVFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYHHPVNIGNPNEVSILDFAREVLDLVKNPKAHIAYKPLPVDDPKI
                     RRPDITKAREILGWEPSVPRAEGMVKTYEYFKQVVK"
     gene            complement(2310..>2524)
                     /locus_tag="PK198_26245"
     CDS             complement(2310..>2524)
                     /locus_tag="PK198_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF42329.1"
                     /translation="LAAPDPQAMLSEKEQEYKEKFANPYRAASRGFVDEVIQPKDTRR
                     KLIKAFAMLGNKVAHLPRKKHGNIPL"
BASE COUNT          523 a          566 c          805 g          569 t
ORIGIN      
        1 cgcgttgtcc aaataccact gcgcggtgcg gcgcaggccg tcttcagcat aaaccgtcgg
       61 cgcccagccc agatcgtttt tcagtttgga agcatcaatg gcgtagcggc ggtcgtggcc
      121 ggggcggtcg gtgacgaagt gaatcaattg gcgggaagta ccggcggcgc ggcccagcaa
      181 ctcgtccatg acgtcgcaca gggcgtgcac cagatcaatg tttttccact cgttgttgcc
      241 gcctatgttg taggtttccc cgatgcgccc ttggtggtag atgagatcaa tggcttcggc
      301 gtggtcttcc acccagtcga ggatggcacg gcggtcctcg tcatcggnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnntg tatgttgttg atcatcaaag gaatgagctt ctccgggaac
      421 tggcaggggc cgtagttgtt ggagcagttc gacagcacga cgggcagtcc gtaggtatgg
      481 tggaaggcgc gtacaaaatg atcggaagcg gctttggagg ccgagtaggg cgagcgcgga
      541 tcgtagggcg tggtttcggt gaagaagccg gtttcgccca gcgagccata cacttcgtcg
      601 gtagagacgt ggtaaaaacg cttgccgtcc atgttgtctt tccagtgttg gcgggcggcg
      661 tgcaggaggc gcacggtgcc gagcacattg gtgtaaatga aggnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnngatggag cggtccacat gcgattcggc agcgaggtgg ataacaccct
      781 ggaaatcata ctgctgaaag agctggtcca aaaacgcctc gtcttcaatg ctgcctttga
      841 cgaaggtgta attcggcagg ttttccacat cgcggaggtt ttcgaggttg cccgcgtagg
      901 tgagcaaatc gaggttgacg atgtggtact gcgggtagcg gtgcagcatt ctgcgcacca
      961 agtgagagcc gatgaatccg gcgccaccgg tgatgaggat ggtcatgttt tgaagatttc
     1021 ggattcaaaa ttcctgattt cggattcctg atttcggatt gagataaatg aaaaaccgaa
     1081 aaataataaa tgtaaaatct aaaatgtaaa agttatcccg cgtgaggggg cgaagggagg
     1141 tgacgaccgg agcacccgac ggcccagcag gatggtggta gcgggcaggc acgccccaaa
     1201 atcttccctg atacttaatg tttacttcac cacttgtttg aagtattcat aggttttgac
     1261 catgccttct gcacggggta cgcttggctc ccagcccagg atttcgcggg ctttggtgat
     1321 gtcgggccgg cggattttcg ggtcgtccac gggcagcggt ttgtaggcga tgtgcgcttt
     1381 tggatttttg acgaggtcga ggacttcgcg ggcaaaatcg aggatagaca cttcgttggg
     1441 attgccgatg ttgaccgggt ggtggtagtc gctcagcagg aggcggtaga tgccctccac
     1501 gaggtcgtcc acatagcaga aggagcgggt ttgggagccg tctccgaata cggtaagtcc
     1561 ctcgccgcga atggcttgcg agaaaaaagc aggcagtgcg cgaccgtctt ccacgcgcat
     1621 gcgcggcccg taggtgttga agatgcgcac gatgcgggtg tccacgccgt ggtaggtgtg
     1681 gtaggccatg gtgatggctt ccatgaagcg tttggcttcg tcgtaaacgc cgcgcggccc
     1741 cacaggattg acattgcccc agtagccctc ggtttgaggg tgttcgaggg ggtcgccata
     1801 gacttcggag gtagaggcga cgaggatgcg ggcgtttttg gctttggcca atccgaggag
     1861 gttgtgcgta cccagagcgc ctactttgag ggtttggatg ggcattttga ggtaatcaat
     1921 cgggctggcc ggcgatgcga agtggaggat gtagtccaaa ttgtcgggga cgtgaacgaa
     1981 cttgctgacg tcgtggaagt aaaactcgaa ctccttgagc ggaaacaggt gagcgatgtt
     2041 gtccatgttt ccggtgagga gattgtccat tccgatgacg tgataccctt cgcggatgaa
     2101 gcggtcggcc agatgggagc cgaggaagcc ggcgacgccg gtgatgagaa ctcttttcac
     2161 aattcttcag gtttgagcgg cgaagttagg ggattttgag agaagcgggg cctaaaaaaa
     2221 tcggcctcgt ttcgagagcg aatcaaggcc gacaaaggaa atagtcgaaa tcgaatggta
     2281 agatacgcaa gttttaaaaa ctgccaaatt cagagcggaa tatttccgtg tttttttctc
     2341 ggcaggtgtg ccaccttgtt gcccagcatg gcaaaagcct tgatgagctt gcggcgcgta
     2401 tctttgggct gtatcacttc gtccacaaac ccgcgcgacg cagcgcggta cggattggca
     2461 aatttctctt tgtattcctg ctctttttcg gagagcatgg cctgcggatc gggtgcggcg
     2521 agga
//
LOCUS       DAORLF010000577         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22428_length_2520_cov_1.515619, whole genome shotgun sequence.
ACCESSION   DAORLF010000577 DAORLF010000000
VERSION     DAORLF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22428_length_2520_cov_1.515619"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>365
                     /locus_tag="PK198_26250"
     CDS             <1..>365
                     /locus_tag="PK198_26250"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRF42330.1"
                     /translation="VLPFLEQSPQWAGALIGGTLHAVGNVAGAGYAVSDEVGQLSLTV
                     KLGRVALLAPALLFYNWLINRKSSGFSAFVLPNYLWGFMVVVALTSLAPVPDVVISTG
                     KLIGKILLTLAMAAIGMKI"
     assembly_gap    366..376
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(517..912)
                     /locus_tag="PK198_26255"
     CDS             complement(517..912)
                     /locus_tag="PK198_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42331.1"
                     /translation="MKNRISPAHLPAIYSAFLLVLSVNAFSQKTVVWKGGTPGQETNW
                     HCPKNWSTHTVPDAFADVVIPDVSTTTRALPEIRMGKVEINSLTIAHSAGLSLGESAQ
                     LVVLDNTEGIEINGFRHNHPLMAIKGVRQ"
     gene            complement(1034..1723)
                     /locus_tag="PK198_26260"
     CDS             complement(1034..1723)
                     /locus_tag="PK198_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42332.1"
                     /translation="MKNTFAILFLLAMAGPMMAQTQCGAFFPFEKGAFWEMTNYNKKG
                     AVSSITGHEIGIVEENNGVWEAQVKSKVSDDKGKVLNEGSYNVKCKDGTLFIDVADML
                     SPEMKEGMGSMEMSFSGDALTLPSRLEVGQALPDANTEVKAGSGGVTIMTLRFDITDR
                     KVVAKETITVPAGTFDCYKITYTFTTKTILSKTFSVTSWHNEKAGVVRSETYDKKGVL
                     ESRSELSKLKL"
     gene            complement(1923..>2301)
                     /locus_tag="PK198_26265"
     CDS             complement(1923..>2301)
                     /locus_tag="PK198_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRF42333.1"
                     /translation="SMHDNLLIIAGHYKGIDERIREEFVTLEISIGDYVLSGGELPAA
                     VLVDAIGRLLPGVLNDETSALFDSFQDDLLAPPVYTRPAEFRGRKVPDVLMSGHFKNI
                     EEWRLEQSLQRTRERRPDLLEEE"
     assembly_gap    2302..2312
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2313..>2520)
                     /locus_tag="PK198_26270"
     CDS             complement(<2313..>2520)
                     /locus_tag="PK198_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRF42334.1"
                     /translation="GLLDVRVHDLREFGLGRHKQIDDYQFGGGAGMVMMAEPLANAIE
                     SLQAERRYDEIIYMTPDGETLRQPL"
BASE COUNT          507 a          675 c          678 g          638 t
ORIGIN      
        1 cagtactgcc ctttctggag caatcgccgc agtgggcggg cgcgctcatc gggggcacgc
       61 tgcacgcggt gggcaatgta gcgggcgccg gttatgcagt cagcgatgag gtaggtcaat
      121 tatcgctgac cgtcaaactg gggcgcgtgg ccttgctggc gcctgccttg ctgttttata
      181 actggctcat caacaggaag tcttcgggtt ttagcgcctt tgtactgccc aattaccttt
      241 ggggatttat ggtcgtggtg gcgctcacct cattggcccc ggtgccggat gtggtcatca
      301 gtacgggcaa actgatcggc aaaatactcc tcacgctggc aatggcagcc atcggcatga
      361 aaatcnnnnn nnnnnngttg tccgccggcg gcgaccgcct cgcggcggcg gacgcacctg
      421 cgaccctcac cctggccgac ctcgtcggca aaccggagcg ctggccggta ttctgtgggc
      481 attggggtgg tggcaggcgt aggaaggaat tgttatttat tgccgcacgc ctttgatcgc
      541 catcagaggg tgattgtggc gaaatccgtt tatttcgatg ccctctgtat tgtccaatac
      601 gaccaactgc gccgactctc ccagactcag acccgcacta tgggctattg tcaagctgtt
      661 gatttctact tttcccatgc gaatttcagg caatgcacgg gtggtagtgg acacgtcggg
      721 aatgacgaca tctgcaaacg catcgggtac ggtatgcgtg ctccagtttt tggggcaatg
      781 ccagtttgtt tcctggccgg gcgtcccacc tttccaaacc actgtttttt gtgaaaaggc
      841 atttacactc agaaccaaca ggaaggcaga gtagatagcg ggcaggtgcg caggcgaaat
      901 tcgatttttc atttttttgt gtttttgacg tgatagaaaa gacgagcttc tttgtggctc
      961 atacctctct atttggcgac acaaaaaaag gatacccttg cgcgttaggc atcacttacg
     1021 tatcatatag ccatcacagt ttcagcttgc tcagctccga gcggctttcc agtacgcctt
     1081 ttttgtcgta ggtttccgac ctcaccacgc cggctttttc gttgtgccag gaagtcacgg
     1141 agaaggtttt ggacaggatg gtcttggtgg taaaagtgta ggtgatctta tagcaatcaa
     1201 aagtacccgc cggtaccgtg atggtctctt tggccaccac tttgcggtcg gtgatgtcga
     1261 aacggagcgt catgatcgtc acgccgcccg aaccagcctt tacttccgta ttggcatcgg
     1321 gcagcgcttg gcccacttcg aggcgggaag gcagggtgag cgcatcgccg gaaaaggaca
     1381 tttccatgct gcccatgcct tctttcattt cagggctgag catgtcggcc acgtcaataa
     1441 acaaagtacc gtctttgcat ttcacgttgt acgaaccttc gttcagtact ttgcctttat
     1501 cgtcgcttac tttgcttttt acctgtgctt cccacacgcc gttgttttct tctacaatgc
     1561 caatttcatg tccagtaatg gaactgacgg cgcccttttt gttgtaattc gtcatttccc
     1621 agaaagcgcc tttttcaaac gggaagaatg cgccgcactg agtctgcgcc atcatcgggc
     1681 cggccatcgc cagtaaaaac aaaattgcga atgtgttttt catggtttgt gtttttggaa
     1741 ttttggaaag aatcatcgtt tggaaatgta aaactttggc tgtaagccac aagccattag
     1801 ctgtaatctt gatttttatt cataataaag tcatcctttc gggaagatca gccggcagct
     1861 tgaggcttgc ggcctacagc ttgtggccgg cagctaacag ccggcagccg ttcccacccg
     1921 ccctactcct cctccagcaa atccggtctg cgctcgcggg ttcgttgcaa cgattgttcc
     1981 aggcgccact cttcgatgtt tttaaaatgc ccgctcatca gcacgtccgg cactttgcgg
     2041 cctctgaact cggcaggacg ggtataaaca ggaggcgcca gcaggtcgtc ctgaaaggaa
     2101 tcgaacaggg cggaagtctc gtcgttcagc acgcccggca gcaggcgtcc gatggcatct
     2161 accagtacgg cagcgggcaa ttcgcccccc gacagcacat agtcgccgat ggaaatttcg
     2221 agggtgacga attcctcgcg gatgcgctcg tctatgcctt tataatgacc ggcgatgatg
     2281 agcaggttgt cgtgcatcga gnnnnnnnnn nncaaaggtt gccgcagggt ttcgccgtcg
     2341 ggcgtcatgt agatgatctc gtcgtagcgg cgctcggcct gaaggctttc aatggcattg
     2401 gccagcggct cggccatcat caccatgccc gcgccgccgc cgaattgata atcgtcaatt
     2461 tgcttgtgcc gccccagtcc gaactcgcga aggtcgtgta cccgcacgtc aagcagaccc
//
LOCUS       DAORLF010000578         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22459_length_2517_cov_2.447197, whole genome shotgun sequence.
ACCESSION   DAORLF010000578 DAORLF010000000
VERSION     DAORLF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22459_length_2517_cov_2.447197"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>955
                     /locus_tag="PK198_26275"
     CDS             128..>955
                     /locus_tag="PK198_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42335.1"
                     /translation="MADLPEMWLWNGVSGIAASLLALAVVLMAYRRFPPRLIQHKNPV
                     FRSVVVYFSASLAVLSVLAVLKTMVVRSDTILGFYPIFSAGKAGLLYVLNIAVWLQLL
                     FVVVHRLLMEVESSGLPSVSRMKSLLAGIAAALPVAYWVLPELSLVSTGLLLLAFLGL
                     FDLFLERRSLNLTWLAVWIAVLSAGAAYGVSSFGKLKIKQSFYFTEAVLSDPQPPKAV
                     GDFRQLARDISEKDTLLTLLSTPVPFTVQEEVLRGGIAPLLERLPYLKDNYRLESIQA
                     "
     assembly_gap    956..994
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <995..2351
                     /locus_tag="PK198_26280"
     CDS             <995..2351
                     /locus_tag="PK198_26280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF42336.1"
                     /translation="IEGRSYEQTEAYRSGKVPDLVILNDAQGYRVLLRIPADLSGNEA
                     SLLWRPDAPPTPDVLSLASLFSGIFILLTALTALWAALGHRLRFLFGASAFSFIDKPS
                     LRNRIQVWLAAFTLGAFVLTGLVSYTFFQRFGIIEPAMMHYYLSALLNLYVFLLLAAL
                     AVAVAVGNTITQPLAVIGNKLEQLKLDRNEPLEWSGQDEIGQLVHAYNRAIEEVERSA
                     ELLRRSEREGAWREMARQVAHEIKNPLTPMKLSIQYLQRAYQNDPEQALPLINNVAGT
                     LIEQIDTLTKIAGEFSNFAQLPRQEKEVFDWAELTRSISRLFELEQAERNMRIVCSTP
                     DNEVFVFADRSQLTRVVNNLVKNAIQAIPEERPGEVSIALRVQNGRALLSVRDNGSGI
                     AADLQAKVFYPNFTTKSSGMGLGLAMCKNIVDASEGQIWFETEENAGTTFFVELPAAQ
                     A"
BASE COUNT          547 a          642 c          670 g          619 t
ORIGIN      
        1 aacccgcctt cgccaaggct tcggcggatg aaacccgaaa ccttgaaccc gaaacaaccc
       61 ccgttccaac ccgaaacctc caaaatccca aactctctcc ttacattagc gcttcaaata
      121 agcgagtatg gcagacctgc ccgaaatgtg gttgtggaat ggcgtatcag gcatagcggc
      181 ttcgctgttg gcattggccg ttgttttgat ggcgtatcgc aggtttcctc cccgcttgat
      241 tcagcataaa aacccagttt ttcgttccgt tgtcgtctat ttttcggcct cgcttgcagt
      301 gttgagcgtg ttggcggttt taaaaacgat ggtcgtccgg tcggatacca tcttggggtt
      361 ttaccccatt ttcagcgcag gaaaagccgg attgctttac gtgctgaaca ttgccgtatg
      421 gcttcagctg ctgtttgtag tggtgcatcg cttgctgatg gaggtagaaa gcagcggtct
      481 gccctccgtt tcccgaatga aaagtttgtt ggccggtatt gcagctgccc tgcccgtagc
      541 atattgggta ttgccggagt tgtcgttggt aagcacagga ctgctattat tggcctttct
      601 cggtttgttc gacctttttc tcgaacgccg ctcgctcaat ttgacctggc tggcggtttg
      661 gatagccgtg ttgtcggcag gcgctgcgta cggcgtcagt agttttggaa agctgaaaat
      721 caagcagtca ttttacttta cagaagcggt tttatctgat ccacagcctc ctaaagccgt
      781 cggagatttt cggcaattgg cccgggacat ctccgaaaaa gataccttac ttacacttct
      841 ttccacgccc gtacccttca cggtacagga ggaagtcctc agggggggga ttgcaccatt
      901 gttggagcgt ctcccctacc tgaaggacaa ctatcgctta gaaagtattc aggcgnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatcga ggggcgctct tacgagcaaa
     1021 cagaagctta ccggagcggc aaagtacctg atttggtcat cctgaacgac gcccaggggt
     1081 acagggtatt gctgcgcata ccggccgatt tatcgggcaa tgaagcttct ttgctatggc
     1141 gacccgatgc gccgccaact ccggatgtac tgtcgttggc atcgcttttt tccggcattt
     1201 tcatcctgct cacggcgctc acggcattat gggcggcact tggccatcgc ctgcggtttt
     1261 tgttcggcgc ctcagcattt tctttcatag acaagccatc gctgcgcaac cgcatacagg
     1321 tctggctggc ggcgttcaca ttgggggcgt ttgtactcac gggcttggtt tcttacactt
     1381 tttttcaaag attcggcatc atcgagccgg ccatgatgca ctattatctc tctgccctgc
     1441 tcaatttgta tgtgttcctg ctgctggcgg ccttagcggt ggcggtggcg gtgggcaata
     1501 cgatcaccca acctttggcg gtcatcggca acaaattgga acaactgaaa ttggaccgca
     1561 acgaaccctt ggagtggagc gggcaagacg aaatcgggca gttggtacac gcctacaacc
     1621 gcgccattga agaggtggaa cgcagtgcgg aattgttgcg ccgctccgaa cgcgagggcg
     1681 cctggaggga aatggcccgg caggtggcgc atgagatcaa aaacccgctg acgcccatga
     1741 aactgagcat ccaatacttg caaagggctt accaaaacga ccccgaacaa gccttgccgc
     1801 tcatcaacaa cgtggcgggc acactcatcg aacaaattga tacgctgacc aaaatcgccg
     1861 gggagttttc caattttgca caattgcccc ggcaggaaaa ggaggtcttt gactgggcgg
     1921 aattgacgcg cagcatcagc cggttgtttg agttggaaca ggcggaacgc aatatgcgga
     1981 ttgtttgctc aacacctgac aatgaggttt ttgtatttgc cgaccgcagt cagctcaccc
     2041 gcgtcgtcaa caatctggtg aaaaacgcca tacaggccat tcccgaagag cggccgggcg
     2101 aggtatccat tgcgctacgg gtgcaaaatg gccgggcttt gttgtctgtt cgggacaatg
     2161 gatcgggcat agcggcagac ttgcaggcga aggtgtttta ccccaatttc accaccaaat
     2221 cttccggcat gggcctgggc ctggcgatgt gtaaaaacat cgtggatgcc tccgaagggc
     2281 agatttggtt tgaaacggag gaaaatgcgg gcactacttt ttttgtggaa ctaccggctg
     2341 ctcaagcctg acggtatcgg cttatggcta ttggccgccg gcggttggcc tgactttcta
     2401 aaagaaaaag gattcatttt caaaagtaaa ctttgaaatt acctttgcag cagtttacaa
     2461 aatagcacta aagcgtatgg cactgcgaga tgatttcccc ccggcaggct ccgacta
//
LOCUS       DAORLF010000579         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22563_length_2509_cov_2.482478, whole genome shotgun sequence.
ACCESSION   DAORLF010000579 DAORLF010000000
VERSION     DAORLF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22563_length_2509_cov_2.482478"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK198_26285"
     CDS             <1..655
                     /locus_tag="PK198_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF42337.1"
                     /translation="FFIRDTSVFVTKESLSSTAVPMNNVFFETTFTNKTLSAVTQAGM
                     VNNLNDGMIWGLLPVLLLSRQFDAAQAGIITAIYPAVWGLGQLFTGKMSDIYSKKNML
                     VIGMMMQGIAILFLPFANKFWTYATISTCIGFGTALVYPTFLSAIAQATNPQQRAESI
                     GTFRLWRDLGYAFGAVLSGFTADVFGLEYAILLIGILTIISSLIIRWRMPGSPSNPT"
     gene            719..2104
                     /locus_tag="PK198_26290"
     CDS             719..2104
                     /locus_tag="PK198_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRF42338.1"
                     /translation="MSSIREVLWEAIRGEEKQYTTGSIRRAIVLLSIPMILEMAMESL
                     FAVVDVFFVSRIGVDAVTTVGLTESVITIVYSLAWGVAMAATAMVARRIGEDKPEEAA
                     RAAVQSIILALSLGLVLGISGFIFADDVLRLMGAEEEVVRSGVLYTRISFASNVIILL
                     LFLLNGIFRGAGDAAIAMYSLWIANAINIILDPLFIFGIGPFPEMGVAGAAVATTIGR
                     SVGIAFQLYILFKGKSVVPIAMRHLRLHWATIKRLINVSATGAGQFIIGSASWIFLMR
                     IISLSGSDAVAGYTIAIRIIVFTILPSWGLANAAATLVGQNLGAGHPERAEASVWKAA
                     WYNTAFLLSVSVIFIAFAPWLIRLFNQDPEVVRAGVLSLRIICTGYLFFGFGMVVTQA
                     FNGAGDTRTPTLLNLLCFWAIEIPTGYLLAVVFDMGLPGVCWAVAGSEALLAVFSILL
                     FKRGKWKNVKI"
     gene            complement(2177..>2509)
                     /locus_tag="PK198_26295"
     CDS             complement(2177..>2509)
                     /locus_tag="PK198_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42339.1"
                     /translation="AAPKGKRGRPAQPKAEAPVVTETAAAPKGKRGRPAQPKAEAPEA
                     LPEIKPIEPLVTEAAYAEASATEQDAAYDQVHLERMEDKIAKLERQILKIQKKLLKMK
                     KRVKYAGK"
BASE COUNT          505 a          608 c          662 g          734 t
ORIGIN      
        1 tttttttatt cgcgacacct ctgtttttgt aacaaaagag agcctttcca gcactgctgt
       61 gccgatgaat aatgtatttt ttgaaacgac ttttacaaat aaaaccctca gcgccgttac
      121 acaggcaggc atggtcaaca acctcaacga cggcatgatc tggggcttgc tgccggtact
      181 gctgttgtcg cggcaatttg atgcggcaca ggccggaatt attactgcca tctatcccgc
      241 agtgtggggc ctggggcagc tttttacagg caaaatgtcg gatatatatt ccaaaaaaaa
      301 tatgctggtg atcggaatga tgatgcaggg cattgccatc ctgtttttgc cgttcgccaa
      361 taaattttgg acgtatgcaa ctatttcaac gtgtataggg tttggaacgg cgctggttta
      421 tcccactttt ttgtcggcca ttgcacaggc cacaaatccg caacaaagag cagagagcat
      481 aggtactttc aggctttggc gcgatctggg ttatgctttc ggggctgttc tttcgggctt
      541 tacagcagat gtgttcggtt tggaatatgc catattattg attggaatac tgacgatcat
      601 ttcgtcgctc atcatccggt ggaggatgcc cggcagcccg tcgaacccga cttaatagtg
      661 gtttgccgaa atgcgaaggc cgcacttcta ttggtaatta cctgaaaacg tattcattat
      721 gtcgagtatc cgggaagtat tatgggaggc catccggggg gaagagaagc aatatactac
      781 cggcagtatc cggcgtgcca tagtactgtt gtccattcct atgattttgg aaatggcaat
      841 ggagtcgctt tttgcggtgg tggacgtgtt ttttgtctcc cgaatcggcg tagatgccgt
      901 gacgaccgtc ggcctcaccg agtcggttat taccattgtt tattcgctgg catggggtgt
      961 ggcgatggcc gctactgcga tggtggcgcg caggatcgga gaagacaagc ctgaggaagc
     1021 cgccagggcc gccgttcagt ccattatact ggcgctgagc ctgggtttgg tgttgggcat
     1081 atccggtttt atttttgccg atgatgtttt gcggctcatg ggcgcggaag aagaagtggt
     1141 gcgcagcggc gttttatata cgcggatttc ctttgcttcc aacgtcatta ttttattgct
     1201 ctttttgctc aacggcattt tccggggcgc cggagatgcc gccattgcca tgtattcact
     1261 ctggatagcc aatgccatca acatcatact cgatccgctt tttattttcg gcatagggcc
     1321 gtttcctgaa atgggcgtgg caggtgcggc agtagctact accattgggc gaagtgtggg
     1381 cattgctttt cagctttata ttctgtttaa aggcaaaagc gtggtgccca tcgccatgcg
     1441 acatctgcgg ctgcactggg ccaccatcaa gcgcctcatc aatgtatctg ccaccggagc
     1501 gggacagttc atcatagggt cggccagttg gattttcctc atgcgcatca tttcgctgtc
     1561 cggctctgac gcggtagccg gctacaccat tgcgattcgc atcattgttt ttaccatttt
     1621 gccgtcctgg ggtctggcca atgcggccgc cactctggtg ggccaaaacc tcggcgcggg
     1681 ccatcctgag cgcgcagaag cctccgtatg gaaagcagcc tggtacaata cagcgttttt
     1741 gctcagtgta tccgtcatat ttattgcttt tgcaccctgg ctcattcgtt tgttcaatca
     1801 agacccggag gtggtacgcg ccggcgtact cagtttgcgc attatttgca cggggtattt
     1861 attttttggc ttcggcatgg tcgttacgca ggcattcaac ggcgccggcg atacccgcac
     1921 gcccaccctg ctcaatttgc tttgtttttg ggccatcgaa attcccacag gctatttact
     1981 ggcggtggtt tttgatatgg ggcttcccgg cgtgtgctgg gccgtagccg gcagtgaggc
     2041 tttattggcc gtattttcta ttttattgtt caaaaggggc aaatggaaaa atgttaaaat
     2101 ataaccttga ttccaataga aaaagccgct tcaatgaccc atcgaagcgg cttttatttt
     2161 atatttaatt catcaattac ttccctgcgt atttcacgcg ctttttcatt ttcagcaatt
     2221 tcttctggat tttgagaatt tgccgttcca atttggcaat tttatcctcc atccgctcca
     2281 aatgtacctg atcgtaagcg gcatcttgct cagtggcaga tgcctctgcg taagcggcct
     2341 ctgttaccaa aggttcaatc ggcttgattt caggcaaggc ctccggcgct tctgccttcg
     2401 gttgtgccgg acgaccgcgc ttgcctttgg gagcggcagc tgtttcggtg acaacaggcg
     2461 cttctgcttt cggttgtgcc ggacgaccgc gcttgccttt cggtgcagc
//
LOCUS       DAORLF010000580         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22757_length_2492_cov_2.101354, whole genome shotgun sequence.
ACCESSION   DAORLF010000580 DAORLF010000000
VERSION     DAORLF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22757_length_2492_cov_2.101354"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2096
                     /locus_tag="PK198_26300"
     CDS             <1..>2096
                     /locus_tag="PK198_26300"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF42340.1"
                     /translation="VHIVLNGQKDRVFTAEINGAFSIPYGSDIKTLTFSYVGFETQDI
                     ALQGPPAKPLRVTLKEAAYQLQEAVVVAGENPAHRIIRQAVKNRDRNNPEKLDAWRYR
                     SFAKVTLKLLPNWAEMELRRKTAESTRKSGKEGAAADTFELKDVHLFLMETLTDHAFQ
                     KPGRKREELLSHRTSGFKDPWFASLATQLQPFSFYRDELPFLEKRYLNPVSPGSTERY
                     RFRLEDTFTEGPDSVFVISFQPRPGTAFAGLKGVLHIHSDGFAIQHLIAESAEKEQIK
                     FHIDQKYSRAQGGQWFPEQLSLVLDAEKYPDPSVGTRMSSRTYIDSVRIRPEFPKGFF
                     SPAETYFVAPDLVPLDSIAQVRPEPLSRTDSMSYVLWDSLGRKANLDGKMRTLEVITE
                     GALPIGKIEWVFTDLIRFNNFEGFNPGLGLRTGRNLSKQFQLYGYGGYAFKAKQWKYA
                     GSLTWFPSAAHRRTSLSVFYNNTLTEPATFDFPLRTQLVNRRYFAGRMDRQESMGAAF
                     SARPLRSLSLRVGLERQRHEPLFEYRYLPPVGEPLSEFRFTEADVFLRYAHGATSLRF
                     LGTETELQSKFPILWLRASRGFGGVWDGQYDYWRLHGALSWRLHHRRWGSTDMMLEGG
                     RVSADVPYAKLFTTLGFGAGWSEVSLNTAFETMQPYEFVSDRMANVYVEHNFNRLSSK
                     NPRFQPQPALIHRMGWG"
     assembly_gap    2097..2120
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2121..2330
                     /locus_tag="PK198_26305"
     CDS             <2121..2330
                     /locus_tag="PK198_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42341.1"
                     /translation="PELHELGVLNRMNRGYFESGLAINSLLRFNYVNIGYIGFGIKAL
                     YRYGPYTLPDWKDNVAVRLTMKFSR"
BASE COUNT          543 a          663 c          719 g          543 t
ORIGIN      
        1 gtacacattg tgctgaacgg ccagaaagac cgcgtgttta cagcagagat caacggggcg
       61 ttttccatac cttatggtag tgacatcaaa accctgactt tcagctatgt aggatttgaa
      121 acgcaagaca ttgccctgca aggcccgccg gcgaaacccc tgcgcgtgac gctcaaagag
      181 gccgcctacc aactacagga ggccgtagtc gtggccggtg aaaaccctgc gcaccgcatc
      241 atccggcagg ccgtgaaaaa ccgagatcgc aacaatcctg aaaaattgga cgcctggcgc
      301 taccgatctt tcgccaaagt gacgctgaaa ctgctgccca actgggccga aatggaactg
      361 cgcagaaaaa ctgctgaatc aacccgcaaa agtggcaaag aaggcgccgc tgccgatacg
      421 tttgaactga aagatgtaca cctgtttttg atggaaaccc tcaccgatca cgcgtttcag
      481 aagcccggac gaaagcgtga ggaattgctc agccaccgca catcgggctt caaagaccct
      541 tggtttgcct cactggccac ccaattgcag cccttttcgt tttaccgcga cgaactgcct
      601 tttttggaaa aacgctacct caatcccgta tcgccgggca gcaccgagcg ctaccggttc
      661 aggctggaag acaccttcac cgaggggccg gacagcgtgt tcgtcatttc gtttcagccc
      721 cggccgggta cggctttcgc cggactaaag ggcgtgctgc acattcattc cgatggattt
      781 gccatacagc atctcattgc agaatcggct gaaaaagaac agattaaatt ccatattgac
      841 caaaaataca gccgcgcgca gggcgggcag tggttcccgg agcaattgag tttagtactc
      901 gacgcggaga aatatcccga tccgtcggtg ggtacccgca tgagttcgcg cacctacata
      961 gacagtgtgc gcatccggcc tgagttcccg aaagggtttt tctctccggc ggaaacgtac
     1021 ttcgtggcgc ccgatctggt tccgctcgac agcatagctc aggtaagacc tgagccactg
     1081 agccgtactg attccatgtc gtatgttttg tgggacagcc tgggccgcaa agccaatttg
     1141 gacggcaaaa tgcgcacgct ggaagtcatt actgaaggcg ccttgcccat cggcaaaatc
     1201 gagtgggtgt tcaccgatct gatacgattc aacaacttcg agggcttcaa tcccggactg
     1261 ggccttcgca ccggcaggaa tctgtcgaag cagttccaac tgtacggcta cggcggctat
     1321 gcgttcaaag ccaaacaatg gaaatacgcg ggttcgctca cttggtttcc ctcggctgcg
     1381 caccgccgca cctcgctgag cgttttttac aacaacacgc tgacagaacc cgctactttc
     1441 gatttcccgt tgcgcacgca gttggtgaac cggcgctatt ttgcagggcg catggatcgg
     1501 caggagagca tgggggcggc attttcggcg cggccgctgc ggtcattgtc gttgcgcgtc
     1561 gggctggagc ggcaacggca cgagcctttg ttcgagtacc gatacctgcc gccggtcgga
     1621 gagccgttgt cggagttcag gttcaccgag gccgatgtgt ttttgaggta tgcgcacggc
     1681 gctacgagcc tgcggttttt gggcaccgag acggagttgc agtccaaatt tccaattctg
     1741 tggctgcggg cttcgcgggg cttcgggggt gtttgggatg gccaatacga ctactggcgg
     1801 ctacatggcg cgctgagctg gcgactgcat catcgccgtt ggggcagcac agatatgatg
     1861 ctggaaggcg gccgggtgag tgccgatgtg ccatacgcca agctgtttac tacgctggga
     1921 ttcggggcgg gctggagcga agtgagtttg aacaccgcgt ttgaaaccat gcagccctac
     1981 gagtttgtgt ccgaccggat ggccaatgta tatgttgagc acaatttcaa tcgcctgagc
     2041 agcaaaaatc cgcgtttcca accgcagccg gccctcatac accgcatggg ctggggnnnn
     2101 nnnnnnnnnn nnnnnnnnnn cccgaattgc acgagttggg ggtgttgaac cgaatgaacc
     2161 ggggctactt cgagagtggt ttggcaatta actcactgtt gcgcttcaat tatgtcaaca
     2221 tcggttacat cggattcggt atcaaggcat tgtatcgcta tggtccctat acgctgcccg
     2281 attggaaaga caatgtggcg gtgcgcctga cgatgaagtt cagcaggtga tgggggctgg
     2341 tgggggcggc tgttagcttt tagctgcgag cttggttgtt tgttcataaa gaggttgtta
     2401 ttttggggaa aaccgaaatc cgcccgttcc tgccggaacg ctaaggtttt taacgcgaat
     2461 ccgccccggc ggacaggttt agcgaagggc gc
//
LOCUS       DAORLF010000581         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22761_length_2492_cov_1.879360, whole genome shotgun sequence.
ACCESSION   DAORLF010000581 DAORLF010000000
VERSION     DAORLF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22761_length_2492_cov_1.879360"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>279)
                     /locus_tag="PK198_26310"
     CDS             complement(<1..>279)
                     /locus_tag="PK198_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF42342.1"
                     /translation="GDPRRDATILYVGEVLPDGSGIVQDNPDILNERYSQKAWTPNHP
                     GLQDNGPSNIRILRYADVLLIAAEALNENDKPAHALTYINLVRARARGT"
     assembly_gap    280..384
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<385..1227)
                     /locus_tag="PK198_26315"
     CDS             complement(<385..1227)
                     /locus_tag="PK198_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF42343.1"
                     /translation="MKKHIIRLFSLLALFLGAAGCQKDFLEVQPQGEINAENYFQTAD
                     HATWAVNAVYNQLRAWDVIAFPYLAMTDIVSDDATKGSFPADAQRIATFDNFTYGPSF
                     PEEVRVAWRGYYRGIFRANSAIEGIPKAQMNEMLRSRLIGEAKFLRAHYYFNLVRWFG
                     DIPLIDKQLTEAEFYTKPRAPKAEVYNLIIQDLKDAIEVLPEKNQYPTADMGRATKGA
                     ARGVLAKVYLTIGDFVNAEQYALQVINSNQYALLPQYNRIFLPEGENSSESVFEIQAT
                     ALEDS"
     gene            complement(1363..>2202)
                     /locus_tag="PK198_26320"
     CDS             complement(1363..>2202)
                     /locus_tag="PK198_26320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HRF42344.1"
                     /translation="VLNSGLDAALNWRGNVGKLGYRVGVLGSTLKNEVKSLGTGNEEL
                     FGVFISGEPATRTEIGGPIGAFYGYRVAGLYQNAEDIAKYPNREPVKPGDLRFEDVDG
                     DGIITPDDRTYLGSAIPNLVYGFNFGFDIAGFDFSADFNGVSGNKILNSKKMARGFGI
                     PNFESSFLARWTGEGTSNSEPRVTNGGYPNFNVSDRFLEDGSFMRLRSVQLGYTLPKA
                     LLSKAGITNLRIYVSGNNVMLWTNYSGYSPEIGSENVLAVGIDGGIYPTARTFLVGLN
                     AGF"
     assembly_gap    2203..2231
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2232..>2492)
                     /locus_tag="PK198_26325"
     CDS             complement(<2232..>2492)
                     /locus_tag="PK198_26325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF42345.1"
                     /translation="NDKIPTGAATPIVSNGLGAVFGTPEQLLFGAGITTLANPFLQWE
                     LSNSTNAGIEIGAIQNRLTLELDYYRRYTEKILVPVPIPDYV"
BASE COUNT          504 a          601 c          694 g          559 t
ORIGIN      
        1 ggtgccgcgg gcgcgggcgc gtaccagatt gatgtaagtg agcgcgtgtg cgggcttgtc
       61 gttttcattg agcgcttcgg cggcaatgag gagtacgtcg gcatagcgaa ggatgcggat
      121 gttgcttggc ccgttgtcct gcaagccggg gtgattgggc gtccaggctt tctggctgta
      181 gcgctcattg agtatgtccg ggttgtcttg cacaatgcct gagccgtcgg gcagcacctc
      241 gcccacatag aggatggtag catcgcggcg gggatcgccn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnggagtc ttcgagtgca gtggcctgaa tttcaaacac
      421 cgactcggag gagttctcgc cttccggcaa aaagatgcga ttgtattgcg gcagcagggc
      481 gtattggttg gaattgatga cctgcaaagc gtactgctca gcatttacaa aatcgccgat
      541 ggtgagatat actttggcca gcacgccgcg ggcagctcct ttcgtggccc ggcccatatc
      601 ggcagtcgga tactggttct tttcgggcag tacctcaatg gcgtctttca aatcctgaat
      661 gatgagatta tagacttcgg ccttgggcgc gcgaggtttt gtgtaaaact cagcctccgt
      721 caactgcttg tctatgagcg gaatgtctcc aaaccagcgc acgaggttga agtaataatg
      781 cgccctcaga aatttggcct caccgatgag cctgctgcgc agcatttcgt tcatctgggc
      841 ttttggaatg ccttcgatgg cggaattggc tctaaaaata ccccggtagt agccacgcca
      901 tgccacgcga acttcttcgg ggaaggaagg cccgtaggtg aagttgtcaa aagtggcaat
      961 gcgctgtgca tcagcgggaa agctgccctt ggtggcgtcg tccgacacga tgtcggtcat
     1021 ggccagatac gggaaggcaa tgacatccca ggcgcgcagt tggttataca ctgcattgac
     1081 ggcccaagtg gcatgatcgg ccgtttggaa atagttttcg gcgttgattt cgccctgcgg
     1141 ttgcacttcc agaaagtctt tttggcaacc ggctgctccc agaaagagcg caaggaggct
     1201 gaatagacga atgatatgct ttttcatagt tgatattttc ttggttaaca attttttttc
     1261 cagcttagtg aaggggtgac tacggggctg acacttgccg agtcacccct tcgctacccc
     1321 ccgaaacccg gaaccttgaa cccggaacct taaacccgaa actcagaacc ccgcattcaa
     1381 acccaccaaa aaggtgcgcg ccgtaggata aatcccgccg tcaatgccca ccgccagtac
     1441 attttcgctg ccaatttcgg gactgtaccc gctgtagttg gtccaaagca tgacgttgtt
     1501 gccgctcaca taaatgcgaa gattggtgat gccggctttg gagagcagcg ctttgggcaa
     1561 ggtataaccc aattgcacac tgcgcaggcg catgaaggaa ccgtcttcca aaaagcggtc
     1621 ggacacgttg aaatttggat aaccgccgtt ggtcacccgc ggttcgctgt tgctcgtgcc
     1681 ttcgccggtc cagcgggcga ggaaggagga ttcaaaattg ggaatgccga atccgcgggc
     1741 catctttttg gaattgagga ttttgttgcc cgatacgccg ttgaagtctg ccgaaaagtc
     1801 gaaaccggct atgtcaaagc cgaaattaaa gccgtaaacg aggttgggaa tggcactgcc
     1861 gaggtaggtg cggtcgtcgg gcgtgatgat gccgtcgccg tccacatcct caaaacgcag
     1921 gtcgccgggt ttcaccggtt cgcgattggg atatttggca atgtcttctg cattttggta
     1981 caaaccggcc acgcgataac cgtagaatgc gccgatgggg ccgccgattt cggtgcgggt
     2041 agcgggttcg ccggagatga atacgccgaa gagttcttcg ttgccggtgc ccaatgattt
     2101 cacctcgttt ttgagcgtag aacccagcac accgacgcgg tagccgagct tgcccacatt
     2161 gccccgccag ttgagggcag catctaatcc gctgttgagt acnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn ncgacataat ccggtatcgg cacgggaacc aggatttttt cggtgtagcg
     2281 gcggtagtag tctaattcta atgtgagccg gttttgaatc gcgcctatct caatgcctgc
     2341 gttggtgctg ttgctgagtt cccattgcag gaaaggattg gccagcgtgg taatgcctgc
     2401 gccaaacaga agttgctccg gcgtgccgaa tacagcgcct aaaccattcg atacgatggg
     2461 cgtagcagcg ccggtgggaa ttttgtcgtt gc
//
LOCUS       DAORLF010000582         2486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22837_length_2486_cov_1.860140, whole genome shotgun sequence.
ACCESSION   DAORLF010000582 DAORLF010000000
VERSION     DAORLF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22837_length_2486_cov_1.860140"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK198_26330"
     CDS             <1..254
                     /locus_tag="PK198_26330"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF42346.1"
                     /translation="GTNNANFATPPDGASGRMQMFLWTEGRAELLTVLEPANIAGGYE
                     TTSAAFGPEITATPIIGNVVIARDGSSQPELCCNAMPPK"
     gene            251..2359
                     /locus_tag="PK198_26335"
     CDS             251..2359
                     /locus_tag="PK198_26335"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF42347.1"
                     /translation="MNGKIALIQRGECFFKEKVRNAQLAGAIAVIICNIDEGLIGMGD
                     VGSVPNVNIPSVMLKNSSCQLIRTAIANGATVQVRLQRPDTGGPSQVDGDFDNGIIAH
                     EYGHGISNRLTGGPSQADCLFNGEQMGEGWSDFFSLITTAKPGDTGGQARGIGNYAVR
                     ASANASGIRRLPYSTDPSVNAQSYDDIIATTAPHPLGEVWVGMLWDLYWRLVDIYGWD
                     EDLVNGDGGNNKAIQLVTDGMKFQNCLPGFIDGRNAIIAADIVNYNGIHECLIWDVFA
                     RRGLGYSAQQGDNFDRNDGFQAFDVKPECIAELKIAKAMTPNINAGENITVTLTATNH
                     KAETATAVRVSDVIPSGAAFVAGSVTGAANFTVDGNMISFQLGDMISGDEQTVTYRLA
                     SSPNLKSVRQLYDDMEQGDGYWGREALEGSNIWDLNDGPASSGDYTWFVPSTNNENDQ
                     VLLLLDPVVVSAAQPIMRFKHRYNTEPTIDGGIVQISTDGGSTWERPETYIFKNGYRG
                     RIYYTAFATPNVQAFWGNSNGFVDTYYDLSAYIGQEIFIRFRFSTDAQATDDPEQGIG
                     WYVDDFELLDMFNYNSEACITSNQGDQACAEAPNKGSIVEPGVSTAVAEQLIGGAKME
                     VFPNPTYDFVNVSFKLDHSSRVNIRLTGTDGRLLRQTSMELGAGTQLLPFSLQGLPQG
                     VYVLHVQTDAGSATEKIVKK"
BASE COUNT          580 a          671 c          673 g          562 t
ORIGIN      
        1 gtggcaccaa caatgccaat tttgccaccc cgcccgacgg cgccagcggc cggatgcaaa
       61 tgttcctgtg gacggaaggc cgagcagaat tgctgaccgt gctggagccg gccaacattg
      121 ccggggggta tgaaaccacc tctgctgctt tcggccccga aatcactgca acgcccatta
      181 taggcaatgt ggttattgcc agagacggca gttcacagcc cgaattgtgc tgtaatgcaa
      241 tgccgccgaa gtgaacggca aaatagccct catccagcgc ggggagtgct tttttaaaga
      301 gaaagtgcgc aatgcccaat tggccggtgc tattgctgtt atcatttgca atattgatga
      361 aggtttaatt ggaatgggcg atgtaggctc tgtaccgaat gtgaacatac ccagcgtgat
      421 gctcaaaaac agttcctgcc agttgattcg caccgccata gctaatggtg caacggtaca
      481 ggtgcgtctg caacgcccgg ataccggcgg cccctcccaa gtggacggcg atttcgacaa
      541 cggcatcatt gcacacgaat acgggcatgg tatttccaac cgcctcacgg gcggcccctc
      601 gcaggccgac tgcctgttca acggcgagca aatgggcgaa ggctggagcg atttcttctc
      661 tctcatcacc actgccaagc cgggcgatac cggcggccag gcgcgcggca tcggcaacta
      721 tgccgtcaga gcttctgcca atgccagcgg gattcgccgc ttgccttatt ctaccgaccc
      781 cagcgtgaac gctcagtctt acgatgacat cattgccacc acagcgcccc acccgctggg
      841 cgaagtatgg gtgggtatgc tttgggattt gtactggcga ttggtggata tttacggttg
      901 ggatgaggat ttggtgaacg gcgacggtgg caacaacaag gccattcaat tagtcaccga
      961 tgggatgaaa ttccaaaact gcctgcctgg cttcattgac ggccgcaacg ccatcatcgc
     1021 tgccgatatc gtcaattaca acggtatcca cgagtgtctg atatgggatg tttttgctcg
     1081 cagaggtctg ggctatagcg cgcagcaagg cgacaatttt gaccgcaatg acggttttca
     1141 ggcttttgat gtaaaaccgg agtgcatcgc cgagctgaaa atagccaaag ccatgacgcc
     1201 caacatcaat gcaggcgaaa acatcaccgt gacgctcacc gccaccaacc acaaagctga
     1261 aaccgctacc gccgtgaggg tgtcggatgt aattccctca ggtgctgctt ttgtagccgg
     1321 ctcggtgacg ggcgctgcca atttcaccgt tgacggcaac atgatctcat tccaattggg
     1381 cgacatgatt tccggcgatg aacaaacggt gacttaccgc ctggcatctt ctcccaatct
     1441 caagtcggtg cgtcaacttt atgacgacat ggaacaaggc gacggctact ggggccgcga
     1501 agcgctggaa ggatccaaca tttgggacct caacgacggc ccggcttcca gcggcgatta
     1561 tacctggttt gtgcccagca ccaacaacga aaacgatcag gtacttctgc tgcttgatcc
     1621 tgtggtggtt tctgcggctc aaccgatcat gcgtttcaag caccgctaca atacagaacc
     1681 taccattgac gggggcatcg tacagatttc gacagatggc ggcagcactt gggagcgccc
     1741 cgaaacttat attttcaaaa acggctaccg cggtcgcatt tactacacgg catttgctac
     1801 gcccaatgtg caggctttct ggggcaacag caacggattc gtggatactt attacgacct
     1861 gagtgcttac atcggtcagg aaattttcat ccgtttccgc ttctctaccg acgctcaggc
     1921 caccgatgat cctgaacaag gcatcggctg gtatgtggac gactttgagt tgttggacat
     1981 gttcaactat aattcagaag cttgcatcac ttccaatcaa ggcgatcagg cttgcgccga
     2041 agcgcccaac aagggcagta ttgtagaacc cggcgtgagc actgccgtag cagagcaatt
     2101 gattggcggc gccaaaatgg aggtatttcc caatccgacg tatgattttg tgaatgtgtc
     2161 cttcaagttg gatcacagta gccgggtgaa cattcgtctc accggaacag acgggcgttt
     2221 gttgcgccag acttcgatgg aactgggcgc cggcacgcaa ttgctgccgt tcagtctgca
     2281 aggtctgccg caaggggtgt atgtgctgca tgtgcaaacc gatgcaggca gcgctaccga
     2341 aaaaattgtg aagaagtaat tcttaaacaa tttataaaaa aggtcgaggc gcccactcgt
     2401 tgggtgtctc gacctttgta tttttgccgc tcgctcgcgc gaggctctac cttcatgttt
     2461 accacgcacg gcgtggtgcg gattat
//
LOCUS       DAORLF010000583         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22884_length_2481_cov_2.141385, whole genome shotgun sequence.
ACCESSION   DAORLF010000583 DAORLF010000000
VERSION     DAORLF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_2481_cov_2.141385"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>689
                     /locus_tag="PK198_26340"
     CDS             <1..>689
                     /locus_tag="PK198_26340"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF42348.1"
                     /translation="TDTATLYVNDAPPLLVHLPDAGICRNMPARLQPRASQGRPPYSY
                     YWSNGATSPTIEVLPPIPTQYSVTITDACGSTASDTVWVQITEPPDAWISGQSEICAG
                     DTAFLSVVLSGTPPWALSYTINGTEQAIISDIQTPIFELPATRQGIYRISEVEDAFCR
                     NAGQGTAEVHVNTIDIQWEVRPVSCFGFADGSIQCGISGGTPPYSTRWSNGIENEWIV
                     QGVRAGIYSIT"
     assembly_gap    690..712
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <713..1514
                     /locus_tag="PK198_26345"
     CDS             <713..1514
                     /locus_tag="PK198_26345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF42349.1"
                     /translation="QSAAIEVPSPTPLAPVSFRCEDLWMPGYTFSAAGGRPPYRYGVF
                     GQANSDASLFRRLVEGQSYHLVIEDEAGCRLEQDWIMPSLRPKAAEIASPLKVRAGAV
                     VQMPTVLLIPEPLVASVRWIPETGLDCSHCLQPYLSALHSDQYTIRIIDIFGCSTETT
                     VNVQVEDAVDVFVPSAFSPNEDLVNDRLVVYAHPLLVKKVLVFRVFDRWGALMYQATD
                     FPPNDEDYGWNGMFRGQLLQPGIYTYMTQVESVSGMVHTFSGDVLLLR"
     gene            1681..>2481
                     /locus_tag="PK198_26350"
     CDS             1681..>2481
                     /locus_tag="PK198_26350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF42350.1"
                     /translation="MPSAAAPRTMTAPKTGWSWRSLAGWSLGIFCFIVFAILLIGSQQ
                     ALNFDQIKELVFSIEFLGFVLCGFAAQMIDGALGLGYGLTCTTALLGFGVPPAMVSSS
                     IHTAEVFATGISGYSHYRFGNVNKRLAKALLWPALAGAVAGSLLLVVAARSYAEWTSL
                     VLGVYCLLLAVRILHKAMQHSVEKKKVRHLSRLAAAGGFLDAFGGGGWGPLVTSTLIS
                     NGRSPRFVIGTVSLVEFFITLATALTFLATLGVTHWHIILGLIAGSAVA"
BASE COUNT          508 a          648 c          681 g          621 t
ORIGIN      
        1 caccgatact gctaccttat atgtgaatga tgcgcctcca ctattggtac atttgcccga
       61 tgcgggcatc tgccggaata tgcccgcccg actacagccg agggcatcgc aggggcggcc
      121 gccttattct tattattgga gcaacggcgc cacttcgccc actatagaag tactgccccc
      181 gatacccaca cagtacagcg tcaccatcac agatgcctgc ggcagtaccg cctccgacac
      241 cgtctgggtg cagatcaccg aaccgccgga tgcgtggatc agtggtcagt cagaaatatg
      301 cgccggcgat acggcctttt tatcggtagt gctcagcggc acgcctccct gggcgctgtc
      361 ttataccatc aacggcacag agcaggcgat catcagcgac attcaaacgc ccatattcga
      421 gctgccggct acccggcaag gcatttacag gatttcagaa gtggaggatg ccttttgcag
      481 gaatgcaggc cagggaacgg ctgaggtaca cgtcaacacc atagacattc agtgggaagt
      541 gcggcctgtg tcttgcttcg gttttgccga cgggagcatt cagtgcggca tctccggcgg
      601 cactccgcct tattctacgc ggtggagcaa cggcattgaa aatgaatgga ttgttcaggg
      661 tgtaagggcc ggaatttaca gcataactgn nnnnnnnnnn nnnnnnnnnn nngcaaagcg
      721 cagccattga agtaccctcg ccgacgcctt tggcgcctgt gtcgttcaga tgtgaagact
      781 tgtggatgcc gggatatacg ttcagtgccg ccggtggcag gccgccttac aggtacgggg
      841 tgttcgggca ggccaattcc gacgcatctc tgtttagaag attggtagaa ggccaaagct
      901 accatttggt cattgaagac gaggccggct gccgcttgga acaagattgg atcatgccgt
      961 cgttgcgccc caaagctgcc gaaatagcct ctccgctcaa agtcagggcc ggggctgtcg
     1021 tccaaatgcc caccgtgctg ctcataccgg aaccactcgt ggcttctgtt cgatggatac
     1081 ccgaaaccgg cttggattgc agtcactgtt tgcaacccta cctctccgcc ttgcattcgg
     1141 atcaatatac gattcgcatc attgacattt ttggttgtag tacagaaacg acggtgaatg
     1201 tgcaggtaga agatgcggtg gatgtgtttg ttccatcggc attttcaccc aatgaagact
     1261 tggtcaatga ccgtttggtg gtgtacgccc atcctttatt ggtaaaaaaa gtgctcgttt
     1321 tcagggtgtt tgatcgctgg ggggcattga tgtatcaggc aaccgatttt ccgcccaacg
     1381 acgaagacta cggctggaac ggcatgttca gggggcagtt gctccagccg ggaatttata
     1441 cctacatgac gcaggtagaa tccgtcagcg gcatggtgca tacgttttcg ggcgatgtgc
     1501 tgttgctgag gtaactctgt ctttaatctc aaagacttac cctgatcttc agcgtcattt
     1561 ctaaaaaaat acgatcctgt tcttgttttt tcgctacctg cgcttacctt tgcatttcct
     1621 attattaagg taggataagt aggtatgtcg tcaacaagca acgaatctgt ctcaacacct
     1681 gtgccgtccg ccgctgctcc acgcaccatg acggcgccca aaaccggctg gtcctggcgc
     1741 agtttagccg gctggagcct tggtattttc tgctttatcg tgttcgcgat tctattgatc
     1801 ggcagccaac aagcgctgaa tttcgaccaa ataaaggagc ttgttttttc cattgaattt
     1861 ctgggatttg tgctctgcgg attcgctgcc cagatgatag acggggcatt aggcttgggc
     1921 tacggcctca cctgcaccac cgctttgttg ggttttggcg tgcctcccgc tatggtcagt
     1981 tccagcatac ataccgccga ggtatttgct accggcatat cggggtattc tcactatcgg
     2041 ttcggcaatg taaataagcg actcgccaaa gcgctgttgt ggcctgcttt ggccggggct
     2101 gttgccggct ccttgttgct tgttgtggca gcccggagct atgccgagtg gacaagcctt
     2161 gtattgggag tgtattgttt gttgctggct gtcaggatat tgcacaaagc catgcagcac
     2221 agtgtcgaaa aaaagaaagt gagacacctg tcgaggctgg cggcggcagg cggttttttg
     2281 gatgccttcg gcggcggcgg ctggggacca ttggtcacgt ccacactcat ttccaacggc
     2341 cgcagtcccc gctttgtcat cggcaccgtg agcttggtgg agtttttcat cacgctggct
     2401 acggcgctca cttttttggc cacattgggc gtcacgcatt ggcacatcat tctgggcctg
     2461 atagccggca gcgcagtggc a
//
LOCUS       DAORLF010000584         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_22936_length_2476_cov_1.977695, whole genome shotgun sequence.
ACCESSION   DAORLF010000584 DAORLF010000000
VERSION     DAORLF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22936_length_2476_cov_1.977695"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK198_26355"
     CDS             complement(<1..598)
                     /locus_tag="PK198_26355"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylceramidase"
                     /protein_id="HRF42351.1"
                     /translation="MQDTPQPPGPTPVQNDIDVWITKADGSALLEKQSSVLGFTTTIN
                     NYPVITIDTTVSYQAVEGFGYTLTGGSAMLINRMDGSSRRQLLDELFRCDNPKMCVSY
                     LRVSLGASDLDEQVFSYNDIPADQQDLSLQQFSLSKDTLHLIPVLKSVLQINPDIKIM
                     ASPWSAPLWMKSNRNSVGGSLLPQYYAVYAQYFVKYIQA"
     gene            complement(691..1890)
                     /locus_tag="PK198_26360"
     CDS             complement(691..1890)
                     /locus_tag="PK198_26360"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRF42352.1"
                     /translation="MKKFLHFLLLLCAVVFTMGSNVSCEKEAPQPEPALPALSVADAQ
                     AERLPVNTSAFFFVTLSEKSTQNVSVAYRLKAGTAQINTDFLDKTGSITIPAGQLSVS
                     LEVVVIGSDLRQPNSDFSIELFQPTGCKLTDSVAVCTLINENGTRLPTNNAGYSTPQT
                     YPDYTLVWADEFDGNALQTSSWNYEIGNGSNGWGNNEHQYYTSNPKNVFVSEGNLIIE
                     ARRERIETFPYTSARLTTKGKREFQFGRIDIRAKLPKGKGVWPALWMLGANISQVGWP
                     RCGEIDIMELIGHEPNKVHGTLHWNGTNGATHQGGSKTLASGDFSDRFHVFSILWQED
                     TISWYVDDALFYSMSRANFGEAVYPFNAPQFFLFNVAVGGNWPGYPDESTVLPQRMFV
                     DYIRIFQ"
     assembly_gap    1963..2149
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2150..>2476)
                     /locus_tag="PK198_26365"
     CDS             complement(<2150..>2476)
                     /locus_tag="PK198_26365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42353.1"
                     /translation="ACCNCFYTTTFKFSKIPGGYSLEVNSPDGAFTKTGSLAGGLPGI
                     PGSGDEGCYAYGGGSGSFSFVPSSTGIPASAPSTKTAILLSGSNTFIGYGALQKEFEI
                     MVLTSNY"
BASE COUNT          536 a          576 c          629 g          548 t
ORIGIN      
        1 tggcctgtat gtacttcaca aaatactggg catatacggc gtaatactgc ggcaacagac
       61 tgccgcccac cgaattgcgg ttgcttttca tccaaagcgg cgcgctccag ggcgatgcca
      121 tgattttgat gtcgggattg atttgcaaca ctgatttcag caccggaatg aggtgcaggg
      181 tgtctttaga gagactgaat tgctggaggc tgaggtcttg ctgatcggcg gggatgtcgt
      241 tgtagctgaa cacctgctcg tcgaggtcgg aggcgcccag actcacgcgg agatagctga
      301 cacacatttt gggattgtca cagcgaaaca gttcatcaag aagttgccgc cgggaactgc
      361 cgtccatccg gttgatgagc atggcgctgc cgccggtgag ggtgtagcca aagccctcca
      421 ctgcctgata ggacaccgtt gtgtctatgg tgatgaccgg gtagttgttg atggtggtcg
      481 tgaagccgag cacagaactt tgtttttcca acagcgcact gccgtcggcc ttggtgatcc
      541 acacatctat gtcgttttgc acgggtgtag ggcctggcgg ctggggcgtg tcctgcatgc
      601 ttttattgca agatgtcatg ggtagccaca aagaggccag caaaaaagcg gatttgaaca
      661 gcatttgtaa catggcggtg cgaaatatgg tcattgaaaa attctgatat aatcaacaaa
      721 catcctttgc ggcaatacgg tgctctcgtc gggatagccc ggccaattgc cgcccacggc
      781 cacattgaaa aggaaaaact gcggggcatt gaacgggtaa accgcctcgc cgaaattggc
      841 cctcgacatg ctgtaaaaca acgcgtcgtc cacataccag gaaatggtgt cttcttgcca
      901 aagaatagaa aacacatgga aacggtctga aaaatcaccc gatgcaagcg tcttactgcc
      961 gccttgatgc gttgccccgt tcgtgccgtt ccagtgcagg gtgccgtgta ctttattcgg
     1021 ctcatggcca atgagttcca tgatgtcaat ttcgccacag cggggccagc ccacctgact
     1081 gatgttggcg cccagcatcc acagcgccgg ccaaacgccc ttgcctttgg gcagcttggc
     1141 gcgaatgtct attctgccaa actgaaactc gcgcttgcct ttggtggtga gtcgtgccga
     1201 ggtgtaaggg aaggtctcga tgcgctctcg ccgggcttcg atgatgagat tgccttccga
     1261 aacaaatacg tttttggggt tggaggtgta atattggtgc tcgttgttgc cccagccgtt
     1321 gctgccgttc ccgatttcgt agttccagga actcgtctga agggcattgc cgtcaaattc
     1381 atctgcccaa accaatgtgt aatcggggta ggtttgcggc gtgctgtagc cggcgttgtt
     1441 cgtaggcagc cgcgtgccgt tttcattgat gagggtacac acagctaccg agtccgttag
     1501 tttgcagccc gtcggctgga acaattctat cgaaaaatcg ctgttcggtt gccgcagatc
     1561 gctgccgatg acaaccactt ccaaactgac cgacaactgc cccgcaggaa tggtgatgct
     1621 tcctgttttg tccaaaaaat cagtatttat ctgcgccgtg cccgctttca gccgataagc
     1681 caccgacacg ttttgggtgg atttttcact caaagtgaca aaaaagaaag ccgaagtatt
     1741 aaccggcaat ctttcggcct gcgcatcggc aaccgacaaa gcaggcaacg cgggttcggg
     1801 ctgcggggct tctttttcac aagaaacgtt ggaacccatc gtaaaaacaa cggcacacaa
     1861 caacagcagg aagtggagga attttttcat caatttctga tttatactcg atagccagtg
     1921 gtttgcgaat tttagttttg caaaccactg gttatcagtt atnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taattcgaag
     2161 ttagtaccat gatctcgaac tctttctgca gagcaccgta tccgataaag gtattggatc
     2221 ccgataggag tatggccgtc ttggtagaag gcgccgatgc ggggataccc gtagaagaag
     2281 gcacaaagct gaaactgccc gagccgccgc cgtaagcgta gcaaccttcg tcgccgctgc
     2341 ccggaatgcc aggtaaaccg ccggccagac tgcccgtttt ggtgaacgcg ccgtcgggag
     2401 agtttacctc caaagagtag ccgccgggga ttttactgaa cttgaaagta gtcgtgtaaa
     2461 aacagttgca acaagc
//
LOCUS       DAORLF010000585         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23306_length_2446_cov_1.696780, whole genome shotgun sequence.
ACCESSION   DAORLF010000585 DAORLF010000000
VERSION     DAORLF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23306_length_2446_cov_1.696780"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..235
                     /locus_tag="PK198_26370"
     CDS             95..235
                     /locus_tag="PK198_26370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42354.1"
                     /translation="MKIANPLYDHAFKYLMSNDKLARKVLSVKDAVLETGLSEEELSV
                     LE"
     gene            415..630
                     /locus_tag="PK198_26375"
     CDS             415..630
                     /locus_tag="PK198_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HRF42355.1"
                     /translation="MPSVKYLLERSAFGVCSYLGERMGIASARVRMYFIYLTFAALGS
                     PILVYLFAAFWLNVRRYIKDNEKVVWK"
     gene            complement(641..1303)
                     /locus_tag="PK198_26380"
     CDS             complement(641..1303)
                     /locus_tag="PK198_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRF42356.1"
                     /translation="MMRYIALLFVFFFLSFAPAQRPPGGLKLALLKYNGGGDWYAVVD
                     ALQNFAKFCNDNLGTNFDTEYATVDVGSPDIFNHPFLFMTGHGNIVLSDADAENLRTY
                     LMGGGFLFVDDDYGMEPYFKVAAKKLFPDRELVELPFEHPIYRQKYEFKKGLPKIHEH
                     DGKPPRGYGIFHEGRLVLFFGVESNISDGWESREVHRTPEELRQQSLRMGANIVQFAF
                     KQ"
     gene            complement(1307..2020)
                     /locus_tag="PK198_26385"
     CDS             complement(1307..2020)
                     /locus_tag="PK198_26385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRF42357.1"
                     /translation="MYLFYTHDIRGQMAFLDEEEARHCAQVLRHKVGDVVHCTDGQGA
                     LLEGRIVELNKKGCTLHIEKEKKTPLERSWRLQLAMAPTKNADRTEWLIEKAVEIGLD
                     SFTPIWCERSERKNLRMDRLEKIALSAMKQSLSLWLPRLETPRSFTDFTGAQTGGRAQ
                     LFIAHCMEDADKMLLQHKCARQRDVCILIGPEGDFSPAEVQAALQAGFEPVSLGTQRL
                     RTETAALSSCLTIHFINNQ"
     gene            2099..>2446
                     /locus_tag="PK198_26390"
     CDS             2099..>2446
                     /locus_tag="PK198_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRF42358.1"
                     /translation="MKILIMIGQLLLSLSILITLHEMGHFMTARWFKTRVEKFYLFFD
                     FLFPLANVLNFALWKKKIGDTEYGIGWFPLGGYVKIAGMIDESNDTAFLDQPPQPWEF
                     RTKPAWTRLIIMLG"
BASE COUNT          589 a          658 c          592 g          607 t
ORIGIN      
        1 ctattgcatt gcagacctgt tagctctgcg gtgcaaccta caactttgcc tcccgccgcg
       61 ttgtttttcg cctacaatac cgtaactttg cctgatgaaa atcgccaatc cgctatacga
      121 ccacgccttt aagtacctga tgtccaacga caaactggcg cgcaaggtgc tgtccgtcaa
      181 agatgctgtg cttgaaacag ggctgtcaga ggaggaactg agcgttttgg agtaattcat
      241 ctatagcctg ctgcgcatca aacgccccag taccttcggc tgccacaatg gagatgaaca
      301 gtgcgaacgc ctgcatcact acataagacc tttatctgtc cgatacagaa caatttacaa
      361 caaaacctgc cggcccgttg ttgtgctgga atttaaccca aaaaaatctg gcctatgcct
      421 tccgttaaat acctgttgga acgttcggct ttcggagtat gcagctacct cggagagcgc
      481 atggggattg cctcagccag agtgcggatg tactttattt atctcacctt cgcggctttg
      541 ggatcgccta ttttggttta cctgtttgca gcattctggc tcaatgtccg gcgatacatc
      601 aaggacaatg agaaagtagt ctggaagtag ttggtgaaac ttactgcttg aacgcaaact
      661 gcacaatatt ggcgcccatg cgcagcgatt gttggcgcag ttcctccggt gtacggtgta
      721 cttctctgga ttcccagccg tcgctgatgt tggattcaac cccgaaaaac aagaccaacc
      781 taccttcgtg aaaaatgcca tagcccctcg gcggtttgcc gtcgtgttcg tgaattttgg
      841 gcaatccctt tttaaattcg tatttctgcc ggtaaatggg atgctcgaaa ggcaattcga
      901 ccaactcccg gtcggggaat aattttttcg ccgccacctt aaaatacggc tccattccat
      961 agtcgtcgtc cacaaacagg aaaccgccgc ccatcagata agtgcgcaaa ttttcagcat
     1021 cggcatcaga caaaacgatg ttgccgtgtc ctgtcataaa caagaaagga tgattgaaaa
     1081 tatccgggct gcccacatcc accgtggcat attctgtatc gaaattggtg cctaaattgt
     1141 cattacaaaa tttggcgaaa ttctggagcg catccaccac ggcataccag tcgccgccgc
     1201 cattgtactt gagcagggcc agtttgagtc cgcccggcgg acgctgtgca ggggcaaaag
     1261 acagaaagaa aaaaacgaac aacagagcga tatagcgcat cataacttat tggttattaa
     1321 taaaatgaat ggtcagacaa gaactcagtg ccgccgtttc ggtgcgaagt cgctgcgtgc
     1381 cgaggctcac cggctcgaaa ccggcttgca gggctgcctg cacttctgcc gggctgaaat
     1441 cgccttccgg gccaatgagg atacaaacgt cccgttggcg cgcgcacttg tgttgcagca
     1501 gcatcttatc tgcatcttcc atgcaatggg cgataaacaa ctgcgccctg cctccggttt
     1561 gcgcccccgt gaaatcggta aaactccgtg gcgtctctaa gcgcggcagc cacagggaaa
     1621 gcgactgttt catggccgag agggcgattt tttccagccg gtccatgcgc agatttttgc
     1681 gctccgagcg ctcgcaccat attggcgtaa aagagtctaa accaatttct acggcttttt
     1741 cgatgagcca ttccgtgcgg tcggcatttt tggtgggggc catagcgagc tgtagtcgcc
     1801 aggagcgttc caatggcgtc tttttctctt tttcgatgtg gagcgtgcag ccttttttat
     1861 tcaactcgac aatccgccct tcgaggagcg cgccttgccc gtcggtacaa tgcactacat
     1921 cgcccacctt gtggcgcagc acctgcgcgc aatgccgggc ctcttcctca tcgagaaagg
     1981 ccatttgccc gcgaatatca tgggtataaa agagatacat gccgcaaagg tgcagaatgc
     2041 cgcagaattg cacaaatgaa tgtacctttg ccctccttaa cgacaagcac aaattcgtat
     2101 gaagatattg attatgatcg ggcagttgct gctcagcctt tccatcctta ttaccctgca
     2161 cgaaatgggc cattttatga cggctcgctg gttcaaaacc agggtggaga aattttacct
     2221 ctttttcgac tttcttttcc ccttggccaa cgtcctcaac tttgcgcttt ggaagaaaaa
     2281 aatcggcgat acagaatacg gcatcggctg gtttccgctg ggcggctatg taaaaattgc
     2341 cggtatgatt gacgaaagca acgacacggc cttcctcgat cagccgccgc aaccctggga
     2401 atttcgaacc aaaccggcct ggacacgcct catcatcatg ctcggc
//
LOCUS       DAORLF010000586         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23364_length_2441_cov_1.326907, whole genome shotgun sequence.
ACCESSION   DAORLF010000586 DAORLF010000000
VERSION     DAORLF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23364_length_2441_cov_1.326907"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    283..315
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<316..>842)
                     /locus_tag="PK198_26395"
     CDS             complement(<316..>842)
                     /locus_tag="PK198_26395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF42359.1"
                     /translation="VLTVHGMVLQKIEKDYAGRYWHLGVRISGANFVPPNALKVEALM
                     SELLDWANGEGRLLPAVVRAAVFHHRFVWIHPFFDGNGRTARLLFNLLLMSDGYPPAI
                     ILKNDRKKYYDALNKANNGDYGKLLLMVMQALERSLDIYLSHLTNSYEDYQPISSIVN
                     EPDFPYGQEYVGLLV"
     assembly_gap    843..934
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<935..1172)
                     /locus_tag="PK198_26400"
     CDS             complement(<935..1172)
                     /locus_tag="PK198_26400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42360.1"
                     /translation="MEWKNYPYESESWEKQWNRIAEKKGRLDQLRPLPAIAVQNIRDS
                     LALEWTHHSNGIEGNTLTLQETKMEDAKFPESELA"
     assembly_gap    1303..1356
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1428..>1636
                     /locus_tag="PK198_26405"
     CDS             1428..>1636
                     /locus_tag="PK198_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42361.1"
                     /translation="MTPKELSIQMLIRLVSSPYHYSRRDLEQLYGISKDTVEDYLRAF
                     RNVGLHLDINDKYRYAVIPDGHFKEL"
     assembly_gap    1637..1655
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1656..2335
                     /locus_tag="PK198_26410"
     CDS             <1656..2335
                     /locus_tag="PK198_26410"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRF42362.1"
                     /translation="PLSDADKAHIGRALDYLPAKDKLYLKNKLDSLYDFQQLGLRSLT
                     RPALERLDRLHKAQRQKRRVVLENYRSRSNDTRNRTVEPFKIDAEKDTLQAFDTEAKD
                     SRHFRLSRIERVTITDEAWEYESHHREKITDVFRIADNDQVQVQLTVDAFAYNSLVEE
                     FPLTLSYLQPDSSPNSWFFQCAVNRDFKGILNFIMANAGHVKIHAPDELRSRACAEAE
                     NILKKFQ"
BASE COUNT          535 a          583 c          571 g          554 t
ORIGIN      
        1 gatgggtata aacgcgcact atattggtgt tggcggaagt ctggcctgtg tcggcactgg
       61 ttttatcgga tttgcaagac gcaaagacag ttgcgaaagc aagcaaaaaa aggattctac
      121 ggatcatgtt ctgacggaat tgattgagcg ggcaaagttg cggatttttg aatgataccc
      181 gatgaaaatt catttcgact ttctttttcg ctctctgttt tccatataat ccaacacggc
      241 ggctttggta gtcagccagt tgcgcccttc tttgaaggca tcnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnaacca ataaaccgac atattcctgc ccataaggga aatccggctc
      361 attgacgatg gaggaaatgg gctgatagtc ttcgtaagaa ttggtcaggt ggctcaggta
      421 gatgtccaaa ctgcgctcca atgcctgcat gaccatgagc agcaatttgc catagtcgcc
      481 gttgttggcc ttgttgagcg cgtcgtagta ttttttgcgg tcgtttttga gaatgatggc
      541 cggcggatat ccatcgctca tcagcagcaa gttgaacaac aggcgcgccg ttcggccatt
      601 tccgtcgaaa aacgggtgaa tccacacaaa gcgatggtga aaaacagcag cccggacaac
      661 agcgggaagc aggcgcccct cgccattggc ccagtccaac aattcgctca tcagggcttc
      721 tactttgagg gcgttgggcg gcacaaaatt ggccccggag atgcgcacgc cgagatgcca
      781 gtacctgccg gcgtagtctt tttcaatttt ttgcagcacc ataccatgca cagtcagcac
      841 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgcca gttcgctttc gggaaatttc
      961 gcatcctcca tcttggtttc ctgcaaggtg agggtgttgc cctctatgcc gttggagtgg
     1021 tgcgtccatt ctaatgccag gctgtcgcgg atgttttgca cggcaatagc gggcaggggc
     1081 ctgagctggt ctaagcggcc ttttttttcg gcaatgcggt tccattgttt ttcccagctt
     1141 tccgattcgt aaggatagtt tttccattcc atgctgcaaa aatagggtgt tttgcattgg
     1201 gatgcaagta tttttgaggc tatccgatat taaggggtga ggggtggtca ggggtgagtg
     1261 tgtgaggggt gagtgtgagg aaaacccgcc tgcgccaagg ctnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg ggttgtttgt ttcgattttt
     1381 cgtcgtacct tagccgaaac atagaacaaa cgctaaaatc cgcttctatg actccaaaag
     1441 aactctctat tcagatgctg atccggctgg tatcatcgcc gtatcattat tcgcgccggg
     1501 atttggaaca attgtacggc atcagcaaag acaccgttga ggactacctt cgggcttttc
     1561 gcaatgtggg cctccacttg gacatcaacg acaaataccg gtacgccgtg attcccgacg
     1621 ggcatttcaa agaactnnnn nnnnnnnnnn nnnnnaaccc ctcagcgacg ccgacaaagc
     1681 gcacatcggc cgcgctctcg actacctgcc ggccaaagat aaattgtacc tcaaaaacaa
     1741 gttggacagc ctctacgact tccaacaatt ggggctccgc tccctcacgc gcccggctct
     1801 ggaacgcttg gaccgcctgc acaaagccca gcgccaaaaa cgccgcgttg tactcgaaaa
     1861 ctaccgcagc cgcagcaacg acacccgcaa ccgaaccgta gaacctttta aaatagatgc
     1921 cgaaaaggac acgctccagg ctttcgacac agaagccaaa gactcccgcc atttccgcct
     1981 ctcgcgcatc gagcgcgtga ccatcacgga tgaagcctgg gaatacgaaa gccatcaccg
     2041 ggaaaaaatc acggatgtgt ttcgcattgc cgacaacgat caggtgcagg tgcaactcac
     2101 ggtggatgct tttgcctata attctttggt ggaggagttt ccgctcactt tgtcgtacct
     2161 gcaaccggac agcagcccca attcctggtt tttccaatgc gccgtcaacc gcgattttaa
     2221 agggattctg aatttcatca tggccaatgc cgggcatgtg aaaatacacg cgccggatga
     2281 gcttcgcagc cgcgcctgcg ccgaagcaga aaatattttg aaaaaatttc agtaactcca
     2341 gggggggtgc ggcgattttg gccctctacc ccaccctatt tttgtaccat ctttatttat
     2401 tcaactcaat cgtatgcgca tctatttgca tttgacgccc a
//
LOCUS       DAORLF010000587         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23451_length_2434_cov_1.676335, whole genome shotgun sequence.
ACCESSION   DAORLF010000587 DAORLF010000000
VERSION     DAORLF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23451_length_2434_cov_1.676335"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>402
                     /locus_tag="PK198_26415"
     CDS             <1..>402
                     /locus_tag="PK198_26415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767353.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRF42363.1"
                     /translation="IGSVVGPHPFPDMVARFQSVISEEIQAQLQQHTGSPRPDAILAC
                     VGGGSNAAGAFYHYLNDERVRLIAVEAAGKGVHSGHSAATSILGTRGIIHGSRTLLMQ
                     TPDGQITEPYSISAGLDYPGIGPLHAHLIATG"
     assembly_gap    403..435
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <436..667
                     /locus_tag="PK198_26420"
     CDS             <436..667
                     /locus_tag="PK198_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42364.1"
                     /translation="ITDEDALQAAFFLARTEGIIPALETAHAFAALDRADILPGQTVV
                     VNLSGRGDKDLAAYMEHFEDPDLKLAPRSSRF"
     gene            697..>1436
                     /locus_tag="PK198_26425"
     CDS             697..>1436
                     /locus_tag="PK198_26425"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRF42365.1"
                     /translation="MNTADFNTNWRKSAAALYRKPTDSKVFGTVELDVTELEKFIAEK
                     RRQGLKITLTHIFLLAIARSYREAVPELNCYVRMGRIVPRRSVDAGLSVLIKNGTEMS
                     SVTVPDADRLTLTELAAWLDAELSRSRRGDENEAMSNKSMLARIPWPFRNWVVHLVRL
                     ITIEWGFSVPFLGISPHSFGSFLLTNTGSVGIDVGYPALLPLANLSAVISMGFPQSKP
                     AVHEGQIVPRRLLTLSAVMDHRVVDALHA"
     assembly_gap    1437..1452
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1535..>2434)
                     /locus_tag="PK198_26430"
     CDS             complement(1535..>2434)
                     /locus_tag="PK198_26430"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRF42366.1"
                     /translation="SLSAIHPVPPPECVGQLTAVLLPTDTNGDGTPDVGLATILATDL
                     VVNPLPHPVQYSLRIYGQLPDIERYGLVLDCSFTDLFDLQGTPIGFKVDVWENGALVD
                     SCATTVLLQDQAGICAQCNCGSTIEGSIKTITGAAMSYYEMSVSPYPEVNVNLQANGS
                     YSFGNIPFGSDITIEPRCNTNYLDGVSTLDLVLIQRHILSVQKLDTPFKLIAADVNQS
                     GNISLLDAILVRRLILHGLDEFPNGKSWRFIPVSYSFPVPTNPWFEAFPEVININDIQ
                     GFNINQNFTAIKLGDVNGSASGM"
BASE COUNT          527 a          677 c          632 g          549 t
ORIGIN      
        1 catcggttct gtggtggggc cgcacccttt tcccgatatg gtggcccggt ttcagtcggt
       61 catcagcgag gaaatacagg cacagttgca gcagcacacc ggcagccccc gccccgacgc
      121 gatattggcc tgcgtaggtg gcggcagcaa tgcagccggc gcattttacc actacctcaa
      181 cgatgagcgc gttcgcctga tcgccgtcga agcggcgggc aagggcgttc acagcgggca
      241 cagcgccgct accagcatac tcggcacacg cggcatcatt cacggcagcc gcaccctgct
      301 catgcaaacg cctgacggcc agatcaccga gccgtattcc atcagcgccg gcttagacta
      361 tcccggcatc gggccgctgc acgcgcacct catcgccacc ggnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnncatca ccgacgaaga tgccttgcag gctgcatttt ttctggcgcg
      481 taccgagggc atcattcccg ccctcgaaac ggcccatgcc ttcgctgctt tagatcgcgc
      541 cgacattctg cccggccaaa cggtggtggt caacttgtcg ggccgcggcg acaaagattt
      601 ggcggcttac atggagcatt ttgaagaccc ggatttgaaa cttgcgcctc gaagctcgcg
      661 gttttaggca gccttcggat tccttacacc aaagatatga acactgctga cttcaacacc
      721 aactggcgca aatctgccgc cgctctctat cggaaaccta cagattccaa agtgttcggc
      781 acggtagagt tagatgtcac cgaattggaa aaattcatcg ccgaaaaacg ccggcaggga
      841 ctcaaaatca ccctcacgca catcttcctg ctggccattg cccgctccta tcgcgaagcc
      901 gtgcccgaac tcaattgtta tgttcgcatg ggccgcatcg tgccgcgccg cagcgtggat
      961 gccggcctca gcgtacttat caaaaacggc accgaaatga gttccgtcac ggtacccgac
     1021 gccgaccgcc tcaccttgac cgagttggcc gcctggttgg atgccgagct gtctcgctcc
     1081 cgtcggggcg acgaaaacga ggcgatgagc aacaaatcca tgttggcgcg cattccctgg
     1141 ccgtttcgca actgggttgt tcacttagtg cggctcatca ccattgagtg gggtttttcc
     1201 gttccgtttt tgggcatcag tcctcacagt ttcggctcct ttttgctcac caataccggc
     1261 agtgtcggca tagacgtagg ttatccggca ctgttgccgc ttgccaatct ttccgccgtc
     1321 atttcgatgg gattcccgca gtccaaaccc gccgtacacg aggggcagat tgttccccgt
     1381 cgcctgctta cactcagcgc cgtgatggat catcgcgtgg tggatgcgct tcatgcnnnn
     1441 nnnnnnnnnn nntatttcaa aaaaatagta cgcgagccgg aattgttgga aaagaagccg
     1501 gagccggttg ccggtcagtg agctgattac cctgctacat ccccgatgcg ctgccgttca
     1561 cgtcgccgag cttgatggcc gtgaagtttt gattgatgtt gaatccctga atatcattga
     1621 tgttgatgac ctccggaaat gcctcaaacc agggattggt cggcacggga aaagaatacg
     1681 atacgggtat aaatcgccag ctttttccat tgggaaactc gtccaaacca tgaagaatca
     1741 gccttctgac gaggatggcg tccagcagcg agatattgcc tgattgattc acatcggcgg
     1801 cgatgagctt gaagggagtg tccagctttt gtaccgaaag tatgtgcctt tgaattaaaa
     1861 caagatcgag cgtgctgacc ccatccagat agttggtatt gcagcggggt tctatggtaa
     1921 tatcactgcc gaacggtatg ttgccaaatg aataactgcc gtttgcttgt aagtttacat
     1981 ttacttcagg atagggcgat acgctcattt catagtagct catagcggcg ccggttattg
     2041 ttttaatgct tccttcaatg gtgctgccac agttgcattg ggcgcaaatt cccgcctgat
     2101 cttgcaaaag caccgttgta gcacaggagt caacgagtgc gccgttttcc catacatcta
     2161 ctttgaagcc tatgggggtg ccttgtaaat caaataagtc ggtaaaacta caatccagta
     2221 ctaagccgta gcgctctata tccggcaatt ggccatagat gcgcagcgag tattgtaccg
     2281 gatgcggcaa tggattgaca accaaatcag tagctaaaat agttgccaac cctacatcag
     2341 gcgtaccgtc tccgttggta tcggtgggca agagtacggc ggttagctgc cccacacatt
     2401 ccggcggcgg cacaggatgt atggccgaca agga
//
LOCUS       DAORLF010000588         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23491_length_2431_cov_1.627104, whole genome shotgun sequence.
ACCESSION   DAORLF010000588 DAORLF010000000
VERSION     DAORLF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23491_length_2431_cov_1.627104"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK198_26435"
     CDS             complement(<1..241)
                     /locus_tag="PK198_26435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HRF42367.1"
                     /translation="MNIKNEVLYRVYFILFIAVIPAALALIYKTAYISYAEGDFWRKK
                     GETLYIESRSIEADRGNILASNGDLLATSIPFFDLF"
     gene            complement(332..649)
                     /locus_tag="PK198_26440"
     CDS             complement(332..649)
                     /locus_tag="PK198_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRF42368.1"
                     /translation="MAKPKGIGYYVSFGFLNSNLILGNLKLLGFLSLLAIIYIANAHY
                     AERNVRRIQILQKEIHELRWRYTSIQAEVMYNSRQSAMSEQLREQGLRPLRDQPKKIV
                     IDP"
     gene            complement(729..1628)
                     /gene="rsmH"
                     /locus_tag="PK198_26445"
     CDS             complement(729..1628)
                     /gene="rsmH"
                     /locus_tag="PK198_26445"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRF42369.1"
                     /translation="MTPYHQPVMAAECIEALAIQPAGVYVDVTFGGGGHSRLILEALG
                     AEGRLFGFDQDPDAVQNVPADPRFVLVQQNFRFLKRFLKLHGIKEVDGILADLGVSSH
                     QLDEAERGFSYRFEAALDMRMNQADECSAATVLNFASADTLQRIFSMYGEVRNSKTLA
                     EHIVSARQTRPLQTIGDLLGVLEPLIRGNRLRYLSQVFQALRIEVNDEMGALADMFSQ
                     AYEILKPGGRLVVLSYHSIEDRVVKNFLKTGNADGEVQKDFYGNIFRPYSFPDSKMSQ
                     PSEEEIRRNPRSRSAKLRVGEKR"
     gene            complement(1625..2083)
                     /gene="mraZ"
                     /locus_tag="PK198_26450"
     CDS             complement(1625..2083)
                     /gene="mraZ"
                     /locus_tag="PK198_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRF42370.1"
                     /translation="MNLMGEYECRIDPKGRLRLPSDLLAQIGEREGLSFVMNRGFENC
                     LTLYPKQVWDRINNEVNSLNQFVKKNREFARYFLRGATPLELDAADRILLPKRLQEWA
                     GIDKDVVLSAINDRIEIWSKDQYDQLLGEEPDDFSDLAEEVLGNVRPDDE"
BASE COUNT          590 a          611 c          584 g          646 t
ORIGIN      
        1 aaaacagatc aaaaaatggt atagaagtgg ccaacaaatc gccgttggag gctaagatat
       61 tgccacgatc tgcttcgatg gatctggatt caatgtaaag tgtttcccct ttttttctcc
      121 aaaagtcgcc ttctgcatag ctgatgtatg cggttttgta aataagtgcc agggcagccg
      181 gaatgactgc tatgaacagg ataaaataca cccgatacag tacttcgttc ttgatattca
      241 tatcgttcgg gttaaaaacg actcaagccg gaggtagacc ggcgatgtcg cgtagtcaat
      301 agtatgttgg tgtgcgtttc ccatttcctt ctcaaggatc aatgactatt tttttcggct
      361 gatcccgcaa cgggcgcaag ccttgttctc tcagttgttc cgacatggcc gattgtctgc
      421 tgttgtacat gacctcggct tgtatggaag tgtagcgcca gcgcaattcg tgaatctctt
      481 tttgcaaaat ctgaatgcgc cgcacattgc gttctgcata gtgcgcattg gcgatgtata
      541 tgatggccaa caaagacaag aatcccagca gcttgaggtt gccgagaatg agattggagt
      601 tgagaaatcc aaagctcaca tagtatccga tgccttttgg ttttgccatg ttatctattg
      661 tttcttgttc cgtgttcaag gttccgcgcc gcatactgcc taccccgcgc cacagcgcgg
      721 ctactcgcct accgcttctc ccccaccctc aactttgcgc ttctcgatct tggattgcga
      781 cgtatctcct cctccgaagg ctgcgacatt ttgctgtcag gaaaagagta cggcctgaaa
      841 atattgccat agaagtcctt ctgcacttct ccgtcggcat tgccggtttt gaggaaattc
      901 ttcaccaccc gatcttcgat agaatggtaa gaaagcacca ccaagcgccc tcccggcttg
      961 agtatttcat acgcctgaga gaacatatcg gccagtgcgc ccatttcgtc gttcacctca
     1021 atacgcagtg cctgaaacac ttgtgagaga taacgcaggc ggttgccccg gatgagcggc
     1081 tccaataccc ccaacaaatc gccgatggtt tgcagcggtc gggtttggcg cgccgagaca
     1141 atgtgttcgg ccagtgtttt ggaattccga acctcgccgt acatactgaa aatgcgttgc
     1201 agcgtgtcgg cggaagcgaa attcagcacg gtggctgcac tgcattcatc ggcttgattc
     1261 atgcgcatgt ccaaagcggc ttcaaaacgg taggagaaac ctctttcggc ttcatccaat
     1321 tggtgtgaag aaactcccaa atctgccaaa ataccatcca cttccttgat tccgtgcagt
     1381 ttgagaaaac gcttgagaaa gcgaaaattc tgctgcacca gtacaaagcg cggatcggca
     1441 ggtacatttt gcactgcgtc cgggtcctga tcgaaaccaa aaagacgccc ttcagcgcct
     1501 aaggcctcca aaatcaggcg actgtgacca cctccgccaa atgtgacgtc cacatacacg
     1561 ccggcaggct gaatggccag cgcttcgata cactcggcgg ccatgacggg ctgatggtag
     1621 ggcgtcattc gtcgtcgggg cggacattgc ccaatacttc ctcagccaga tcggaaaaat
     1681 cgtccggttc ttcgccaagc agttgatcgt attggtcttt cgaccagatt tcaatgcggt
     1741 cgttgatagc ggaaagcaca acgtctttgt ctatcccggc ccattcctgc agtcttttcg
     1801 gcaggaggat gcgatcggct gcgtctaatt cgagcggggt ggcgccccga aggaagtagc
     1861 gggcaaactc gcggtttttt ttcacgaact gattgagact gttgacttca ttgttgatgc
     1921 ggtcccacac ttgcttggga tacagcgtca ggcagttctc aaacccgcga ttcatgacaa
     1981 aagacaggcc ctcccgctct ccgatctgag cgagcagatc ggacggcaaa cgcaggcgcc
     2041 ctttcgggtc aattctgcat tcgtattcgc ccatcaagtt cataaaatcg cgttttattt
     2101 cttttcgggt tcaaagttaa gggattgaaa cacactttac cactttttac cacttttttt
     2161 tcaaaaaaaa ttttccacaa tgcactgcaa agcagacaat tggaaaaaca atcactttag
     2221 cagttctatt ttatcacctt tttgggtgta ttttaagagt aaaaacagca aaatgttgcc
     2281 ggctttttac ggtatggcgc tccattttgt ggtaaaaagt gggaagacag agtcatcatc
     2341 taccgagagg gagaaaagat gatgagcatt cgcgactttc agcgtctgaa agcagcacct
     2401 gttttagttt tttgtttttt tgtaagcggg t
//
LOCUS       DAORLF010000589         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23599_length_2423_cov_1.635980, whole genome shotgun sequence.
ACCESSION   DAORLF010000589 DAORLF010000000
VERSION     DAORLF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23599_length_2423_cov_1.635980"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PK198_26455"
     CDS             <1..944
                     /locus_tag="PK198_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF42371.1"
                     /translation="NDDVLDYQRKFVADNPNSLTAALISAGLPMEYPDFEGDEQAVQM
                     AKWRYTKAHFFDNINLGDPRLLRTPFLFERVDYYVNKLQIQHPDTLAIAIDEVLEKMR
                     PAEETFKYYLIHFLNFYARSNIVGMDAVYVHIADKYYAASQAPWTNEDQLKKIVENAT
                     ELRPLLIGKIAPNVTLLDRAGKNVTLHNIESEYIVLYFWRYDCGHCQKTLPEMKAFYE
                     KFKDKGVKLVSVCAKFTDEVPKCWEYIDEKEIGDWLHLTDPFHRSKYMTIYSIKTTPQ
                     IYVLDREKRILSKRIGAEQLEEVIDRIIEMKQKEGGR"
     assembly_gap    1023..1042
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1114..1611
                     /locus_tag="PK198_26460"
     CDS             1114..1611
                     /locus_tag="PK198_26460"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF42372.1"
                     /translation="MKTIFFIRHAKSSWDQPGLSDRERPLNERGLRDAPFMAKMLAGK
                     GFRLDHIVSSPATRAFTTAGYFAQAFGIPQEQIEVNREIYEAWPNEIMRIVQQLPDEY
                     NTVAIFGHNPTFTTMANHFASQFLDYLPTCGIARVEADISSWKQLNEESGKLVEIFFP
                     KQFAR"
     gene            1698..1994
                     /locus_tag="PK198_26465"
     CDS             1698..1994
                     /locus_tag="PK198_26465"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRF42373.1"
                     /translation="MIITASSCKPSPTLPVDEALLIKVLADVHIAEVAVQNFAGTHKD
                     SIKRIYYQQIYDIHGISETDFRASLQVLSDHQEKMEKLYKKVEKHLESMDKPKQ"
     assembly_gap    2034..2084
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2085..>2423
                     /gene="paaI"
                     /locus_tag="PK198_26470"
     CDS             <2085..>2423
                     /gene="paaI"
                     /locus_tag="PK198_26470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HRF42374.1"
                     /translation="RWLGIEVLETGPGRCVLQMTLRAEMLNGFGIAHGGITYALADSA
                     LAFASNSHGRKAVSVETSISHTKALHEGERLTATAEEEHLSEKIGIYRVRVGNERDET
                     VAVFKGVVYR"
BASE COUNT          631 a          652 c          596 g          473 t
ORIGIN      
        1 tcaacgacga tgtgctggat taccaacgga aattcgtagc cgacaacccc aacagcctca
       61 ccgcagcgct catttccgcc ggcctgccga tggaataccc cgattttgaa ggcgacgagc
      121 aagccgtgca aatggccaaa tggcgctata ccaaagcaca ctttttcgat aacatcaacc
      181 tcggcgatcc gcgcctcctc cgcacgcctt tcctctttga acgggtggac tattatgtga
      241 acaagctcca gatacaacat cctgataccc tggccattgc catagatgag gtattggaaa
      301 aaatgcgccc tgcagaggaa actttcaaat actacctcat tcacttcctc aatttttacg
      361 cccgttccaa catcgttggt atggacgccg tgtatgtaca cattgccgac aaatactatg
      421 ctgccagtca ggcgccctgg accaacgagg atcagctcaa gaaaatagtg gaaaacgcca
      481 ccgaactgcg ccccctgctc atcggcaaaa ttgcgcccaa cgtaacgctg ctcgaccgcg
      541 ccggcaaaaa cgtgacattg cacaacattg aatctgaata cattgtgctc tacttctggc
      601 gctacgactg cggccattgc caaaaaacgc tgccggaaat gaaggcgttt tatgaaaaat
      661 tcaaagacaa gggggtgaag ctcgtctcgg tctgtgccaa gtttacagac gaagtgccca
      721 aatgttggga gtacattgat gaaaaggaaa tcggcgactg gctccacctc acagatccat
      781 tccaccgctc gaagtacatg accatttaca gcatcaaaac cactccgcag atttacgttc
      841 tggaccgcga aaaacgcatc ctcagcaaac gcatcggcgc cgagcaattg gaggaggtga
      901 tagatcggat tattgagatg aaacagaagg aaggagggag gtaagcggtg ggcggtaggc
      961 ggtgctaagc gcggcttgta gcttgaagcc agtagccagc agccgttcca acccgaaacc
     1021 cgnnnnnnnn nnnnnnnnnn nnccacctac ccacgcctgt ggcgtggtgc ggtggataaa
     1081 acccgaaacg gtcaaaacaa aaaacagcga tagatgaaaa ccatattctt catacgccac
     1141 gccaagtcga gttgggatca gcccggcctg agcgaccggg agcgaccgct gaatgagcgc
     1201 ggcctgcgcg atgcgccgtt tatggccaaa atgctggccg gtaagggctt ccggctcgat
     1261 cacattgtga gcagcccggc aacgagggcc ttcactacgg cggggtattt cgcgcaggca
     1321 ttcgggattc cgcaggagca aattgaggtg aatcgcgaaa tttatgaggc atggcccaac
     1381 gagatcatgc gcatcgtgca gcaactgccg gacgagtaca atacggtagc gatttttggg
     1441 cacaatccaa ctttcaccac gatggccaat cacttcgctt cccaattttt agactacctg
     1501 cccacctgtg gcattgcccg tgtggaggcc gatatttcga gttggaaaca actgaacgag
     1561 gagagcggta agttggtgga gatatttttt ccaaaacaat tcgcccgatg atgcgccggt
     1621 ttacaggctt gcggtcgggt gtatgtttac taaactttgg agatttagca aacataatac
     1681 atctgggttt cgccattatg atcatcacag cctcgtcctg caagccctcc cccaccctgc
     1741 ctgttgatga agcgctgttg atcaaggtat tagcagatgt acacattgcc gaagtagccg
     1801 ttcaaaactt tgccggcacg cacaaagaca gcatcaaacg catttattac caacagattt
     1861 acgacataca cggcatttcc gaaaccgatt tcagagcatc gctccaggtg ctgtccgacc
     1921 atcaggaaaa aatggaaaag ctctataaaa aggtggaaaa gcacctcgaa tctatggaca
     1981 aacctaaaca gtagccatgc agcatcgccc ctcgcccgcc gaaataaccg aacnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccgct ggctgggcat
     2101 cgaagtgctg gaaaccggcc ccgggcgctg cgttttacaa atgaccctcc gcgccgaaat
     2161 gctcaacggc ttcggcattg cgcacggcgg catcacctac gccttagccg acagcgcgct
     2221 ggcctttgcc tccaattcgc atgggcgcaa agccgtgtcg gtagaaacct ccatatccca
     2281 taccaaagcc ctgcacgaag gagagcgcct caccgccaca gccgaggaag aacacctgag
     2341 cgaaaaaatc ggcatctacc gggttcgggt cggcaatgag cgcgacgaaa cggtggcggt
     2401 atttaagggg gtggtgtacc gga
//
LOCUS       DAORLF010000590         2422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23608_length_2422_cov_2.511196, whole genome shotgun sequence.
ACCESSION   DAORLF010000590 DAORLF010000000
VERSION     DAORLF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23608_length_2422_cov_2.511196"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..>1969
                     /locus_tag="PK198_26475"
     CDS             49..>1969
                     /locus_tag="PK198_26475"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRF42375.1"
                     /translation="MTYRSLPAIYPSLRKFKVWMMYLLLLVGANAQHLSAQVFTASNL
                     PAAIPDGGFACWALPGQTSSTINVTGIPGNIINPGDVTIHFRLAHAWAGDIEVSIVPP
                     GGSAIVLISRLGSTQCGSNNDYDQANTLSFNAANANTIPLVNPIPAGNYLPTGSSYSG
                     AEPAGNLNNLTGRPFNGNWMLRVKDGSGSNVGELIFFSIEMNVTNCPAAGSIWYVDAS
                     AASGGNGTSWNCAFQNLQDAINAASSGHEVWVKAGTYKPTAYPAGCTGCSSSRDYTFH
                     LKNGVKIYGGFNGMETMLSQRNWTANVTTLSGDIGTANDASDNCHHVVVSISDANMTQ
                     LDGFTVSGGNANEPGTVSVESKTIERYNGGGMYAASSPLTIANCIFDNNSSTVYAGGT
                     YFFSSNASLSNCTFSNNSANYGGGAAVGLGSPTFTNCTFSTNNATNNGGGMHVISSSP
                     STSPILVNCAFVNNSAYEGGGYHTISSGTYLTNCTFTGNTATGGGGGMYNYSTSLPVL
                     DNCVFSGNSAGFGGGVYTSNSSSAPEFNNCLFSGNKASTVGGGMLNSNSTPAKLNNCT
                     FSGNLSNNNGGAVYNSNTSNAVFTNCTIWNNRSGTTTGSADASLFNNNSTPVITYSLI
                     QNHNPSGAGNLNGITN"
     assembly_gap    1970..1984
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1985..>2422
                     /locus_tag="PK198_26480"
     CDS             <1985..>2422
                     /locus_tag="PK198_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRF42376.1"
                     /translation="LDPATAPSTSGNFRLSDCSPAANVGNNVGVSATDLDGNPRIFNT
                     TVDLGAYERQTNSTAPATWYADADGDTFGNPAVSVVSCNQPTGYVSNNLDCNDSNAAI
                     NPNTLWYLDADNDGYYTGTGVTQCASPGAGYRYTGILGGGDCND"
BASE COUNT          597 a          702 c          582 g          526 t
ORIGIN      
        1 cgggcggtaa accacccatt tttcagccat attaataaac catttgtcat gacttacaga
       61 agcctgcctg ccatctaccc ttccctccgt aaattcaagg tttggatgat gtatttgtta
      121 ctgcttgtcg gcgcaaacgc acaacatctg agcgcacaag tattcacagc aagtaatctc
      181 cccgcagcta tacccgatgg cggttttgcc tgttgggcgt tacctggcca aacatccagc
      241 accatcaacg tgacgggcat tcctggaaac atcatcaacc ccggcgacgt aaccattcat
      301 tttcgactgg cacatgcgtg ggccggcgac attgaagtga gcatagtgcc tcccggcggc
      361 tcggccattg tacttatcag ccggctgggt agcactcaat gcggcagcaa caatgactat
      421 gatcaagcca atacgctgtc ttttaatgca gccaatgcca acaccatccc attggtaaat
      481 cccatacccg caggtaatta tctgcccacg ggcagctcat actcaggcgc cgaaccggca
      541 ggtaatctaa ataatttgac aggccggcct ttcaatggaa attggatgct tagagtaaaa
      601 gatggatcgg gatccaacgt aggcgagtta atttttttta gtatcgagat gaacgtcacc
      661 aattgcccgg ctgccggctc catctggtat gtggatgcct cggcagcgag cggcggcaac
      721 ggcacttcat ggaactgcgc tttccaaaac ttacaggatg ccatcaatgc agcaagcagc
      781 gggcatgaag tgtgggtgaa agcgggaacc tataaaccca ccgcatatcc ggcagggtgt
      841 accggatgca gttccagcag ggactatact tttcacctga aaaacggcgt aaaaatctac
      901 ggaggcttca atggcatgga gaccatgctc agccagcgta actggacggc caatgtgacc
      961 accctcagcg gcgacatcgg cacggcgaac gatgcctccg acaactgcca ccatgttgtc
     1021 gtatcaatca gcgatgccaa catgacccag ttggacggtt ttactgtttc gggtggcaat
     1081 gcgaacgagc cgggcacggt ttcagtcgaa tcgaaaacta ttgagcgcta caacggcgga
     1141 ggaatgtatg ctgccagttc gcccctgaca atagccaatt gcattttcga caacaactca
     1201 tctaccgtct atgccggtgg tacatatttc ttttcgagca atgccagcct gagcaactgc
     1261 acgttttcca acaattcggc aaactatgga ggcggagcag ccgttggtct tggttcaccc
     1321 acctttacca attgcacctt ttccacgaat aacgccacca acaatggtgg cggaatgcac
     1381 gtcatcagtt cgagtcccag cacctcccca atactagtta actgcgcatt tgtcaacaac
     1441 tcggcgtatg agggcggggg gtaccatacc atatcttccg gtacgtacct gacaaattgc
     1501 actttcaccg gaaataccgc taccggaggg ggcggaggga tgtacaacta cagtacttct
     1561 ctccccgtat tggacaactg tgtgttttcc ggcaacagcg caggattcgg cggcggagtt
     1621 tataccagta acagctcatc agcccctgaa tttaacaact gcctgttctc cggcaataag
     1681 gcaagtactg ttggcggtgg catgcttaat tccaattcga cccccgccaa gttgaacaac
     1741 tgtacctttt ccggcaacct ttccaacaac aatggcggcg cagtgtacaa cagcaacact
     1801 tccaatgccg tttttaccaa ctgcaccatc tggaacaacc ggtcaggcac gacgactggt
     1861 tcggcagacg ccagtctttt caacaacaat tccacacccg tcatcaccta ctccctcatc
     1921 cagaaccaca acccctccgg cgcgggcaac ctcaacggca ttaccaatgn nnnnnnnnnn
     1981 nnnnctcgac ccggctacgg cgccttcaac gagcggcaac ttccgcctgt cggattgctc
     2041 ccccgccgcc aatgttggta acaatgtggg cgtatcggcc accgacttag acggcaaccc
     2101 gcgcatcttc aacaccaccg ttgacctcgg cgcgtatgaa cgccaaacca acagcacggc
     2161 gcccgccacc tggtacgccg atgcagatgg cgataccttc ggcaacccgg ctgtgtcggt
     2221 agtgagttgt aatcagccga cgggttatgt gagcaataat ttggactgca atgacagcaa
     2281 tgcggccatc aatcccaata cgttatggta cctcgatgcc gacaacgacg gctattacac
     2341 cggcacgggc gtgacgcagt gtgcctcacc cggcgcaggc tatcgctaca cgggtattct
     2401 gggcggcggc gattgtaatg at
//
LOCUS       DAORLF010000591         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23700_length_2415_cov_2.051695, whole genome shotgun sequence.
ACCESSION   DAORLF010000591 DAORLF010000000
VERSION     DAORLF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23700_length_2415_cov_2.051695"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK198_26485"
     CDS             complement(<1..540)
                     /locus_tag="PK198_26485"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HRF42377.1"
                     /translation="MNSYDEYEENLNIKLTARRVEDNSLVHANTTTKKDGPFYCPETY
                     EELIVRKCIEKRDHFAYKARQSPVGSKESDLHKNCKKEICTILQNRFPEGKWEEEREN
                     FKKDDEKGFEKVKPDISGRIGNKGVIIEVQASVLSIKKIIHRTDQYSMRGGYILWIVP
                     LEEELGTEDFRPRLFERYLH"
     gene            845..1846
                     /locus_tag="PK198_26490"
     CDS             845..1846
                     /locus_tag="PK198_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF42378.1"
                     /translation="MREHLQLSGYAPSTVISYVRAVRDLMEQTGRVPLELSEQEVISH
                     LNTWRDERGLSPSALNTHIFGILYLYREVYKDKKVRIDIPNPGRSKTIGDILTITEVR
                     ELFRACHYPKQSAVLQLLYDTGLRAREVARLRLGDFDKANSVLYVRYGKAVVEALRRY
                     FEIERPTDWLFEGSEKGEPQSVLEYLGRYTHKSAISNHRLRQVSPQGVRFSYKDYRQG
                     GAAQEMTLDGVEFLRRWCLHILPPGYRRMRHYGILSNAAKAQALAACRQALGMPPVVP
                     SSRKELRALALAKLRQRYQWDKCPCCKEGRRIRIGLVPAQCRAPPPGELPQWLPLEH"
BASE COUNT          584 a          565 c          602 g          664 t
ORIGIN      
        1 atgtaagtat ctttcaaaaa gtcttggtct aaaatcttct gttcctaatt cttcttctaa
       61 tggaactatc cataaaatat atcctccacg cattgaatat tggtcagttc tatggataat
      121 tttctttatt gaaagaacac ttgcttgaac ttcaatgatt actcctttat ttccgattct
      181 tccgctaata tcaggtttta ccttttcaaa ccccttttca tcatccttct taaaattctc
      241 tctttcttcc tcccattttc cctctggaaa tctattttgt aggattgtac aaatttcttt
      301 tttacagttt ttgtgcaaat cgctttcttt acttccaact ggactttgtc ttgctttata
      361 tgcaaagtgg tctcgctttt caatacactt tctaacaatt aattcttcat atgtttctgg
      421 gcaataaaat gggccgtctt ttttagttgt cgtatttgca tgaacaaggc tattatcttc
      481 cactcttcga gcagtcaatt tgatgtttaa attttcttca tattcatcgt aagagttcat
      541 gtttatcgct ttttattttt ttttggttgc ttccaacgtg agcactcacg cataccgccc
      601 gccagcgttg gctttgagcg ttgggctttg gcggcggttg gcgttgagtg cattattgaa
      661 ctaagccacc ggcaacatct tcgctcagag gaccctatta cgatcaaaag tagaaaaaaa
      721 ggcataactt gaaggaattt attaaccaaa attttcaagc catgccaaaa aaaagcttat
      781 tgatctgacg cagaagagcg tatttcaaac gagtcgcccg gagtacgggg gggtattgtc
      841 gcaaatgcgg gagcatctac aattgagcgg atatgctccc agtacggtga tcagctatgt
      901 tcgagcggtg cgtgatctga tggaacaaac aggtcgggtg ccgctggagt tgagcgaaca
      961 ggaggttatt tctcatctca acacctggcg ggacgaaagg ggcttgagtc cgtcggcgct
     1021 caacacgcac atatttggga tattgtacct gtaccgggag gtgtacaaag acaaaaaagt
     1081 gcgcatagat attcccaatc cgggtcggag caaaacgatc ggggatatcc tgacgataac
     1141 ggaagtgaga gagttgttca gagcgtgtca ttatcccaag cagtcggcgg tattgcaatt
     1201 gctgtatgat acgggtctgc gggcgcggga agtagcgcgg cttcgactgg gagattttga
     1261 caaagccaac agtgtgctgt atgtgcgcta tggcaaagca gtggtggaag cgctccggag
     1321 atattttgaa atagaacgcc cgacggactg gctctttgag ggcagcgaaa aaggagagcc
     1381 gcagtcggtg ctggagtatc tggggcgata cacgcataag tcggcgatca gcaatcaccg
     1441 gctgcgacag gtatcgccac agggagttcg tttttcgtac aaagactacc ggcagggcgg
     1501 ggccgctcaa gagatgactt tggacggagt agagtttttg aggcgctggt gcttgcacat
     1561 tttgccgccg ggataccgtc ggatgcggca ctacggaatt ttgtccaatg ctgccaaagc
     1621 acaggcattg gccgcctgcc gacaggcatt gggaatgccg ccggtcgtgc cttcatcgcg
     1681 caaggaactg cgggcgctgg cgctggctaa gctccggcag cgctaccaat gggacaagtg
     1741 cccctgctgc aaggaaggta ggcgcatccg tatcggcctc gtacccgctc agtgtcgcgc
     1801 tccgccgccg ggagaactgc cccaatggtt gccgcttgag cattgaggct catttttttg
     1861 aaggatgccc gccatccgcc gggaatgttt tgcacttacc cgccgatttt gagcattttg
     1921 tgcgctgcgg catcgatttt tcggtcggtt tttcagtcgc tcaacggtaa gtaagcgccg
     1981 cgccgcccgc caaagggctt agccgactga gaaaaacgga ttgacaggct cggcataaat
     2041 gtttcaaatt ccatagacgc agtatcgggc ttgtcgccgg ccctggggtg ttccgcaaga
     2101 ggctccgcac aacaaagtgc aaccgcacaa cacgtcggcg ggtggggacg tgctgcgcgc
     2161 gtttatcccc acccgccgac gtgtttactg ttgtgcactg aatgttggca ccattattat
     2221 ttatccgatg tcacaaaaga ctgcattata cttcgcacca atagggtttt gtgcatttta
     2281 tccatcctat cttgaaaact tgcacgaaac ctattggaat agagctatag taacctctat
     2341 tgacaatatt ggcatacgaa cgaaccacaa gagcaaaaac acattccatc aatcacagta
     2401 cagtggggtg gtgca
//
LOCUS       DAORLF010000592         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23809_length_2405_cov_1.940000, whole genome shotgun sequence.
ACCESSION   DAORLF010000592 DAORLF010000000
VERSION     DAORLF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23809_length_2405_cov_1.940000"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1349
                     /locus_tag="PK198_26495"
     CDS             120..1349
                     /locus_tag="PK198_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRF42379.1"
                     /translation="MARTLKLGDVDNGAKPKDSQAFFAPFHSTMNPVLQKKNMFRDEA
                     NSAWRGFREHPGSEVRDLQNFLNSAGFLRRPDAPGVFGYATHAGLRLFQEYVRTVEKD
                     ATIGIPDGVAGPKVQEHINRWKQNNLKCKWSEYTTEAPSPEYVLWFSALEKVKQHFIS
                     TSSPVLEEIQKFSKPSDTLKISQWDFDPKHIHLIGIRRNHDLRADVRANDDLFILLIS
                     GMVFKFWGSTDPNPKMALAEGNRTDEAYLTEGQHLYRFGWHKISDEKKIYRALRPATV
                     GVLVFRDRTGANALTEANVKAGVSGPNATINIHWSGIGSSNFSAGCQVIAGDSYINHA
                     NEVVSCRGFAARSYDELAAGKTRGAYNVISDLVLAYAPAGVNLVRYTLGRDAHLNLDA
                     SLGKDFGAKTLEKMRAG"
     gene            complement(1359..>2036)
                     /locus_tag="PK198_26500"
     CDS             complement(1359..>2036)
                     /locus_tag="PK198_26500"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF42380.1"
                     /translation="GIGNTIDRMNMSLSLFREEGVPILGVLVNKVRQDKYDDVRAHLE
                     KRFREMNIPLLGVLPYDQSLSFPIMEAINQAVDGEVVFNEHKLNNRIEEIIAGSLVDT
                     EEFSTFQNLLLVVSYRRLDEAMERIKAITLEKKLEQPMLSGVIVTSDGRHSFLHEQAA
                     ARHPYFAEHEIPVISTSLDTYGSVVKISRIEVKINSRTPWKVERAIRLINEYVDVDDI
                     IRRIERG"
     assembly_gap    2037..2087
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2088..>2405)
                     /locus_tag="PK198_26505"
     CDS             complement(<2088..>2405)
                     /locus_tag="PK198_26505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42381.1"
                     /translation="RRRGLVTGYCKPMGQRFVDVGNGEVADKDAVLFAQSLGFDIEPK
                     IHSPVIILPGLTRNYINHTEEFDFKDRIRHAADYLQSTCDIVVYEGTGHPGVGSVIDL
                     SNA"
BASE COUNT          571 a          633 c          594 g          556 t
ORIGIN      
        1 atatgaacac ttccggcata agacgcctcc attgctgcat tttcatgtaa ttttcaacca
       61 caaatgtcta attttcaatg aatgtttgta cctttcagcc acttaaaatc caatacctta
      121 tggcacgcac cctgaaactt ggcgatgtag acaatggcgc caaacctaaa gattcacagg
      181 cgtttttcgc gccctttcat tctaccatga atcctgtttt gcaaaagaaa aacatgttcc
      241 gcgacgaagc caactccgct tggcgcggtt ttcgggagca tcccggctcc gaggtacgcg
      301 acttgcagaa tttcctcaac tcggcagggt tcttgcgccg ccccgatgcg ccgggtgttt
      361 tcggatatgc cacccatgcc ggcttacgac tgtttcagga gtatgtccgc acggtagaaa
      421 aagacgctac cataggtatc cccgatggcg tagcagggcc gaaagtgcag gagcacatca
      481 accgttggaa acaaaataac ctgaaatgca agtggagcga gtacacaacc gaggctccat
      541 ctccggaata tgtgctgtgg ttcagcgctt tagaaaaggt gaaacaacat ttcatcagca
      601 cttcatcgcc agtattggaa gaaatacaaa aattcagtaa acccagcgac acgctcaaaa
      661 tttcgcagtg ggatttcgac cccaaacaca ttcacctcat cggcatccgg cgcaaccacg
      721 atttgcgggc cgatgtgcgg gccaacgacg acctgttcat cctgctcatt tcgggcatgg
      781 tgttcaagtt ttgggggtcc accgatccca atcccaaaat ggcgctggcc gagggcaatc
      841 gcacggatga agcctacctc actgaggggc agcatttgta tcgcttcggc tggcacaaga
      901 tcagcgacga gaaaaaaata taccgcgccc tgcgcccggc cacggtgggc gtactcgtgt
      961 tccgggatcg taccggcgcc aacgccctca ccgaggcaaa tgtcaaagca ggtgtgagcg
     1021 ggcctaatgc caccatcaat atccactggt cgggcattgg cagttccaac ttttcggcgg
     1081 gctgccaggt cattgcgggc gacagctaca tcaatcacgc caacgaggtg gtttcctgcc
     1141 ggggatttgc cgcccgcagc tacgacgaat tggctgccgg caaaacacgg ggcgcttata
     1201 atgtcatctc cgatctggta ttggcttatg cgccggcggg ggtcaacttg gtgcgctata
     1261 cgctgggccg tgacgcgcat ctcaatctgg atgcttcttt gggaaaggat tttggagcaa
     1321 aaacgctgga gaagatgcgg gctgggtgag tcagtacttc agcccctctc aattctccga
     1381 ataatatcat ccacatccac atactcgttg atgaggcgaa tggcgcgttc taccttccaa
     1441 ggggttcggc tgttgatctt gacctcaatg cggctgattt tgaccaccga accataggtg
     1501 tctaaagagg tggaaataac cggtatctcg tgctctgcaa aataaggatg ccgggcggcg
     1561 gcctgttcgt gcaaaaagct atgccttccg tcgctggtga cgatgacgcc ggagagcatg
     1621 ggctgttcta attttttttc taatgtaatg gctttgattc tttccatcgc ctcgtccaaa
     1681 cggcgataac tcaccacgag cagcaggttt tggaaagtgc tgaattcttc ggtgtccacc
     1741 aatgagccgg caatgatttc ttcgatgcgg ttattaagtt tgtgctcatt gaacaccact
     1801 tcaccgtcca ccgcctgatt gatggcttcc atgataggaa aagacaagga ttgatcgtaa
     1861 ggcagcacgc cgagcagagg gatgttcatt tcgcggaagc gcttttccaa atgagcgcgc
     1921 acgtcgtcgt atttatcctg acgcaccttg ttgaccaata cgcccaatat cggcacacct
     1981 tcttcccgaa acagagacag gctcatgttc atgcggtcaa tggtattgcc gatgccnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc gcattcgaca
     2101 ggtcaataac gctgcccacg ccgggatgcc cggtgccttc atataccaca atatcgcaag
     2161 tactctgcaa ataatcggcc gcatggcgaa tgcgatcttt gaaatcgaat tcttctgtgt
     2221 gattgatata attgcgggtg aggcccggca ggataatgac cgggctgtga attttaggtt
     2281 caatatcgaa acccagagat tgggcaaaaa gaacggcgtc tttatcggcc acctcgccat
     2341 tgcccacatc cacaaagcgc tgccccatag gtttgcaata gccggtcacc aagccccttc
     2401 gcctg
//
LOCUS       DAORLF010000593         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_23846_length_2402_cov_1.807414, whole genome shotgun sequence.
ACCESSION   DAORLF010000593 DAORLF010000000
VERSION     DAORLF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23846_length_2402_cov_1.807414"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PK198_26510"
     CDS             complement(<1..1195)
                     /locus_tag="PK198_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42382.1"
                     /translation="MKQILTILMAAAAFLSFGQSNQADFENLVQLGEFYSKNIDCTGD
                     DFIQTVDRLRTRNLNHIVDALIAVGKADQKLLSKEFLSKPSDQELKYWYVLREIHYNL
                     QPDNANPKTSVEVARQTLEDDIDHRWLLDNYYYRINIGVAKMFNDKDLSKTDIRLDEY
                     GLKTDTEKATLFFSTSNALIQRFQVLKMIKNYDQLLEYVARMPTFNGRPYYEYTAFGF
                     EDFEWIGYNKTESYKTRHLGSLYSALAGHMSALAEKGKNEELRDLYFNSILSMPEYFK
                     YSGGLEKNLQELYKELKTPAQPAGISMLGLKIGDSPKTVEQIKLKVDAREKNMIKFKT
                     SDGNDFSVTVQKGKIVYMENDWLQNPKATQPLYSDFRFGHTTLREIRARFGTNGFVHQ
                     NRRSFT"
     gene            1302..>1928
                     /locus_tag="PK198_26515"
     CDS             1302..>1928
                     /locus_tag="PK198_26515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRF42383.1"
                     /translation="MRSFRFLTTVAFCTVFIWGKLSAQAPMPGILDEQVRARVVNEVL
                     EDRFNNLLPQLMRREGIDMWVLISREYNEDPVMKTMLPAEWLSARRRTVMVFFDRGPG
                     QELEKSAIARYDVGLLKAEWDLNVYPDQWEALVEMIKARNPKKIGINTSDTYGLADGL
                     VHTEYQLLMQKLPAAYKSRVVSAEKLAIAWLETRSEKEMALYEQICRIS"
     assembly_gap    1929..2052
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2053..>2402
                     /locus_tag="PK198_26520"
     CDS             <2053..>2402
                     /locus_tag="PK198_26520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRF42384.1"
                     /translation="VQRADPANFDHLRTFSKRPQGQVILPGDLLHVDFGITYLRLNTD
                     MQQHAYVLRPGETEVPEALKKAFRQGNRLQDILVQQFSTGRTGNEMLARALAQAKAEG
                     IEGSIYSHPIGFHG"
BASE COUNT          555 a          562 c          552 g          609 t
ORIGIN      
        1 tcgtgaacga ccttcggttt tggtgtacaa aaccattggt gccaaaccgc gctcttatct
       61 ctcgaagcgt ggtgtgaccg aatcgaaaat cggaataaag cggctgtgtt gctttggggt
      121 tttgaagcca gtcattttcc atataaacga tttttccctt ttgtacggtg acggagaagt
      181 cgttgccgtc ggaggtttta aattttatca tattcttctc ccgcgcatcc actttaagct
      241 tgatttgctc tactgtcttg gggctgtctc cgattttgag tccgagcatc gaaatgccgg
      301 ccggttgggc aggcgtcttc aattctttgt acagttcctg caagtttttt tccaaacctc
      361 ccgagtactt gaaatattca ggcattgata aaatggaatt gaaatataaa tcacgcagct
      421 cctcgttttt gcctttttcc gccaatgcag acatgtgccc ggccaatgct gaataaagac
      481 ttccaagatg cctcgtcttg taagattcgg ttttattgta gccgatccac tcaaagtcct
      541 caaaaccaaa agcagtgtat tcataataag gcctcccgtt aaaggtcggc atacgggcta
      601 catactcgag caattggtca taatttttta tcatcttaag tacctgaaac ctttggataa
      661 gcgcgttgga agtcgagaag aacagcgtcg ctttttctgt gtctgttttt aatccatact
      721 catcaagtcg gatgtctgtt ttgctcaagt ctttgtcatt gaacatttta gccacaccta
      781 tatttattcg gtagtaataa ttgtccaaca accagcgatg gtcaatatca tcctccagcg
      841 tctgtctggc cacttccaca ctcgttttgg gatttgcgtt atcaggctga aggttataat
      901 gaatttccct cagcacatac cagtatttca gttcttgatc tgaaggcttc gaaagaaatt
      961 ctttcgacag gagtttttga tcggccttgc ccactgcaat caaagcgtca acgatgtgat
     1021 tgagatttcg ggttctcagc cggtcaacgg tttggataaa atcatcgccg gtgcaatcaa
     1081 tgtttttgga atagaactcg ccaagctgta cgaggttttc aaaatcagcc tgattgcttt
     1141 ggccaaaaga caggaaggcg gctgcggcca ttaaaatagt caatatctgc ttcattggcg
     1201 ttttgtttag tatggatgca aaagtaagtc aatactttgg aatatcaatc cggtagttgc
     1261 cgaagctctt tatcttcgcg caaacaatca tcacattggc catgcgttca ttccgatttt
     1321 tgacaaccgt cgccttttgt acggtcttca tttggggcaa actttctgct caggcgccca
     1381 tgcccggcat cctcgacgaa caggttcgcg cccgtgtcgt caacgaggta ttggaagacc
     1441 ggttcaacaa ccttttgccg caactcatgc gccgcgaggg catagatatg tgggtgctca
     1501 tatcgcggga gtacaacgaa gaccccgtga tgaaaaccat gttgcccgcc gagtggctct
     1561 ctgcgcgccg ccgcacggta atggtctttt ttgatcgcgg accgggacag gaattggaaa
     1621 aatcggccat tgcccgctac gacgtgggcc tactgaaagc cgagtgggac ctcaatgtgt
     1681 atcccgatca atgggaggcg ctggtagaaa tgatcaaagc gcgcaatcct aaaaaaatag
     1741 gcatcaatac ctcggatacc tacggactgg ccgatggact ggtgcatacc gagtaccaat
     1801 tgctgatgca gaaactgccc gctgcgtaca agagccgtgt cgtgtcggca gagaaattgg
     1861 ccattgcctg gctcgaaacc cgcagcgaaa aagaaatggc cctgtacgag caaatctgtc
     1921 gcatatcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnacgtgcag cgcgccgatc ctgctaattt cgaccacctg cgtaccttca
     2101 gcaagcgccc gcaaggccag gtaatcctgc ccggcgactt gctgcatgtg gacttcggca
     2161 tcacctatct gcgcctgaac accgatatgc aacagcacgc ttatgtgctg cgtccgggcg
     2221 agacggaagt gccggaagcg ctcaaaaagg cattccggca gggcaatcgc ctgcaagaca
     2281 ttctggtgca gcaattcagt acaggccgca ccggcaacga gatgttggcg cgggcattgg
     2341 cgcaggccaa agcagagggc atcgagggca gcatttattc acatcccatt ggttttcatg
     2401 gc
//
LOCUS       DAORLF010000594         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24040_length_2385_cov_1.828755, whole genome shotgun sequence.
ACCESSION   DAORLF010000594 DAORLF010000000
VERSION     DAORLF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24040_length_2385_cov_1.828755"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="PK198_26525"
     CDS             complement(<1..2112)
                     /locus_tag="PK198_26525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF42385.1"
                     /translation="MQIAAFSVKNYQFTLIVFVMLAALGLSSLLNMPKAEDPEMDAPM
                     FIITAIYPGAGPADMERLVADPIERKVSELSDIKLIETNIDDGVAVLRVEYVYSSNAE
                     EKYQEIVREVNSVESELPDDLYELRVNRISPSDVNILQIALVSENAPYRVLQEQADRL
                     REALERIKTMKNAKTWGLPEQQVRITLQLDKMAQSRIPLSRVMGALQSESVNIPGGNI
                     YLSARKYNVKTSGDYASLDEIRRTVVYSDGTKIIYLGDIAEVDLVDAPETHITRFNGH
                     RAVLVTAAQKSNQNIFAVREAYTPVLAAFEKQLPPNIAMIRHFDQADSVGSRLGRFAK
                     DFLIAILLVSLTLLPLGFRASLVVMISIPLSIAMGLTGLSLLGYSINQLSIVGFIIAL
                     GILVDDSIVVVENIERHLRMGKSKKQAAIEATQQIGLAVVGCTVTLVLAFLPLLFLPE
                     ASGDFIRSLPVAVVATVLASLFVSLTIVPFLSSRILGLKHDARGNLFMRGLNWLIQGT
                     YARLLDKALIYPKTTLLVALLLFGGVLSLVPKVGFSLFPASEKPMFTVNVSLPEGSAI
                     MATDSVVRHVESVLGEHPKVRFYTANTGKGNPRIYYNEIQRNEAANYGQVFVQLLPDV
                     QPKEKEAIIDELRERFIYYPNAKIEVKNFEQGPPVDAPIAIRVFGEDLDTLRKVATQV
                     EDIISGTPGTIYVNNPLSTLRT"
BASE COUNT          497 a          723 c          624 g          541 t
ORIGIN      
        1 ggtgcgcagg gtcgaaagcg ggttgttcac atagatggtg ccgggcgtgc cgctgatgat
       61 gtcttccact tgagtggcca ctttccgcag ggtgtccaag tcttcgccga acacccgaat
      121 ggcgatgggc gcatccacgg gcgggccttg ctcaaagttt ttcacctcaa ttttggcatt
      181 cgggtagtat atgaaccgct cgcgcaactc gtctataatg gcctcttttt ctttgggttg
      241 tacatcgggc agcaattgca caaaaacctg gccgtagttc gcggcttcat tgcgctgtat
      301 ctcgttgtag taaatccggg gattgccctt gccggtattg gcggtgtaaa acctcacttt
      361 cgggtgctcg cccagcacgc tttccacatg ccgcaccacg ctgtcggtcg ccatgatggc
      421 cgagccttcc ggcaaactca cattcaccgt aaacatgggc ttttctgatg ccggaaacag
      481 gctgaagcct actttaggca ctaagctcaa taccccgcca aacagcagca gcgctaccaa
      541 aagggtcgtt ttgggataga tcagggcctt gtccaacaag cgggcgtagg tgccctgaat
      601 gagccagttg aggccccgca tgaagaggtt gccgcgcgcg tcgtgcttca gccccaatat
      661 ccggctcgac aaaaacggca cgatggtcag cgatacaaac agggaggcca gcaccgtagc
      721 caccaccgct accggcaggc tgcggatgaa atcgccggag gcttcgggca ggaacagcag
      781 cggcaaaaac gccaaaacga gcgtcactgt acagcctacc actgccagcc ctatttgctg
      841 cgtagcctct atggcagctt gttttttgct cttgcccatg cgcaggtgcc gctctatgtt
      901 ttccactact acaatggagt cgtccaccag aatgcccagc gcaatgatga agcccacaat
      961 gctgagctga ttgatcgaat accccagcaa actcaggccg gtcagcccca tagcgatgga
     1021 cagcggaatg gaaatcatga ccaccaacga ggcccggaaa cccagcggca acagggtgag
     1081 cgataccagc aaaatggcga tgaggaaatc tttggcaaaa cgccccaggc gactgcccac
     1141 cgaatcggcc tgatcgaaat ggcggatcat ggcgatgttg ggcggcaact gcttttcaaa
     1201 ggcggccagc accggcgtgt atgcctcgcg cacggcaaag atgttttggt tcgacttctg
     1261 cgcggcggtc actaatacgg ccctatgccc gttgaagcgg gtgatgtggg tttccggcgc
     1321 atccaccaaa tccacctcgg ctatgtcgcc cagatagatg attttcgtgc cgtccgaata
     1381 cacgacggtg cggcgtatct cgtccaatga ggcgtagtcg ccgctggttt ttacattgta
     1441 ttttctggca ctcagataga tgttgccacc cggaatgttc acgctttcgc tttgcagcgc
     1501 gcccatcacc cgcgaaagcg gaatgcggct ttgggccatt ttgtccaatt gcagcgtgat
     1561 gcgcacctgt tgttcgggca gtccccaggt tttggcattt ttcatcgttt tgatgcgctc
     1621 cagcgcctcg cgcagacggt cggcctgttc ctgcagcacc cggtacggcg cattttccga
     1681 caccaacgct atctgcaaaa tattcacatc agagggcgaa atgcggttca cgcgcagctc
     1741 gtacaggtcg tccggcagct cgctttccac gctgttcacc tcccgcacaa tctcctggta
     1801 tttctcctcc gcattgctcg aatacacata ctccacgcgc agcaccgcca cgccgtcgtc
     1861 tatattagtt tctatcagtt ttatgtcgct cagttcactc actttgcgct ctatgggatc
     1921 agccaccaag cgctccatat cggcaggccc cgcgccgggg tatatggccg tgatgatgaa
     1981 catcggcgca tccatctccg ggtcttcagc tttgggcatg ttgaggagcg acgataagcc
     2041 cagcgccgcc agcatgacga acacaatcaa ggtgaattgg taattcttta ccgaaaaggc
     2101 tgctatctgc atggtgtttt cttttgcctg atttttacga ttttttcttt ttcgggcgcc
     2161 cccgacacta cctgccctcc ccaaaccttc ccacatcagg caggcgggcc gggctatccg
     2221 ctgcatggcg gccaccccgc agagctgtta gctttatccc gctattgcag cgctaaagct
     2281 aacaggctct atacccgacc gccgccattc cgctgctatc cctggcgcgc attaccgcac
     2341 cacccgcacc cgcgaacgtt ccgacaaata cggcgaaccc gctgt
//
LOCUS       DAORLF010000595         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24107_length_2380_cov_2.215054, whole genome shotgun sequence.
ACCESSION   DAORLF010000595 DAORLF010000000
VERSION     DAORLF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24107_length_2380_cov_2.215054"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PK198_26530"
     CDS             <1..1451
                     /locus_tag="PK198_26530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRF42386.1"
                     /translation="MERILNYIGGSLVPPRSGRFLDNVNPATGQVYSHIPDSDASDVA
                     EAVAAAQQAFPDWAAAVPEVRFRILNRIADLIEADLESFALAESNDNGKPLSVARSVD
                     IPRAVSNFRFFATAAMQFASEAHPMPGQAVNYTLRQPLGTVGCISPWNLPLYLFTWKI
                     APALAAGNCVVGKPSELTPMTAFLLAKVCIEAGLPAGVLNIVHGLGPSAGQAIVEHPS
                     VKAISFTGGTATGRHIAATAAPMFKKLSLELGGKNPSIVFADCDFEQAVSTAARSAFS
                     NNGQICLCGSRMYVQKPIYEQFRDELVRRVSALRTGDPLAADSDLGALLSRSHWEKVQ
                     GYLALAKEEGGRILCGGEPIRPEGRCSEGFFLSPAIIEGLDAQCRTNQEEIFGPVVTI
                     QAFETEEEVLQWANGTDYGLAANVWTQNLSRAHRVAERLQMGIVWINCWMLRDLRTPF
                     GGVKHSGIGREGGWEAMRFFTEPKNVCVKYGK"
     gene            complement(1480..1553)
                     /locus_tag="PK198_26535"
     tRNA            complement(1480..1553)
                     /locus_tag="PK198_26535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1517..1519),aa:Asn,seq:gtt)
     gene            1648..>2117
                     /locus_tag="PK198_26540"
     CDS             1648..>2117
                     /locus_tag="PK198_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRF42387.1"
                     /translation="MSLLTVGTVAFDDIQTPFGEARNAIGGAATYISLSASYFCKDIQ
                     LVSVVGDDFPESELSHLSSRGVDLAGLQVKEGEKSFFWAGRYHGNMNDRDTLDTQLNV
                     LADFDPVLPDHFRQSPFVMLGNLTPAVQMRVLDQMEERPRLVTLDTMNFWMNTA"
     assembly_gap    2118..2127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2128..>2380
                     /locus_tag="PK198_26545"
     CDS             <2128..>2380
                     /locus_tag="PK198_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRF42388.1"
                     /translation="SLREVLRRVDVLTINDEEARQLSGEHSLVKAAKIIHQMGPRYLV
                     IKKGEHGALLFEGDSLFFAPALPLSEVVDPTGAGDTFAGG"
BASE COUNT          527 a          690 c          636 g          517 t
ORIGIN      
        1 tcatggagcg cattctcaat tacatcggag gcagccttgt gccaccgcgg tcgggccgtt
       61 ttttagacaa tgtcaacccc gccaccggcc aggtttacag ccacatccct gattccgacg
      121 cctccgatgt agccgaggcc gttgccgctg cacaacaggc tttccctgat tgggccgccg
      181 ccgttcccga agtgcgtttc cgcatactca accgcattgc cgacctcatc gaagccgatc
      241 tggaaagctt tgccctcgca gaaagcaacg acaacggcaa acccctatcc gtggcccgca
      301 gcgtggacat tccgcgcgcc gtttccaatt ttcgattctt tgccacggcc gccatgcagt
      361 ttgcatccga agcgcatccc atgcccgggc aggccgtcaa ctataccctg cgccagccat
      421 tgggcacggt gggctgcatc tccccctgga acctgccgct ctacctcttc acctggaaaa
      481 tagcgcccgc tctggctgcc ggcaattgcg tggtgggcaa accctccgaa ctgacgccca
      541 tgaccgcctt tttactcgcc aaagtctgca tagaagcagg gttgcccgcc ggcgtactca
      601 atattgttca cggcctcggc ccctccgccg ggcaggccat tgtcgagcat ccctccgtca
      661 aagccatttc tttcaccggc ggcaccgcta ccggaaggca catagcagcc acggcggcgc
      721 ctatgttcaa aaaactgtct ttagaattgg gcggcaaaaa ccccagcatc gtctttgctg
      781 attgcgattt cgagcaggcc gtttccacag cagcgcgttc ggccttttcc aacaacgggc
      841 aaatttgcct ctgcggctcc cggatgtatg tgcaaaagcc tatttacgag caatttagag
      901 acgagttggt tcgccgtgtc tctgctttgc gcaccggcga cccgctggcc gcggattccg
      961 atctcggagc attgctctcg cgttcgcact gggaaaaagt gcagggatat ctggcgctgg
     1021 caaaggaaga aggcgggcgc attttgtgtg gcggagagcc gatccggccc gaaggccgtt
     1081 gttcagaagg tttttttcta agcccggcca tcatcgaagg cttggatgct caatgtcgta
     1141 ccaatcagga ggaaattttc ggcccggtag tgaccatcca agcgtttgaa accgaagagg
     1201 aagtgctgca atgggccaat ggaaccgact acggactggc cgccaatgtg tggacgcaaa
     1261 acttgagccg cgcacatcgg gtagctgagc gcctgcaaat gggcattgtg tggatcaatt
     1321 gctggatgct gcgcgacctg cgcacgccgt ttggcggggt taaacattcg ggcatcgggc
     1381 gcgagggagg ctgggaggcc atgcgttttt ttaccgagcc gaaaaatgtg tgtgtgaagt
     1441 atgggaaata aaaaaagccc tgctttcgca gagcttttgc gcctcagcaa ggacttgaac
     1501 cttggaccta cggattaaca gtccgccgct ctaaccgact gagctactga ggcaattttg
     1561 cccccttttt aaaagagcga cgcaaaggta taaccatctc cagaaaaatg caatctttgc
     1621 agcgaaaaaa aattaaaaaa aatttcaatg agcttactca ccgtaggaac cgtagccttc
     1681 gacgacatcc agacgccgtt tggcgaagcg cgcaacgcta ttggcggcgc agccacatac
     1741 atctcattat cagcttctta tttttgcaaa gacatccaat tggtgtcagt tgtgggagat
     1801 gatttccccg aatcagagct gtctcacctc agcagccggg gcgttgatct ggcggggctg
     1861 caagtgaaag aaggcgaaaa atctttcttc tgggcaggtc gctaccacgg aaatatgaac
     1921 gatcgcgata cgttggacac ccaactcaac gtattggccg attttgaccc cgtgctgccc
     1981 gatcactttc ggcagtcgcc ttttgtgatg ctgggcaatc tcaccccggc agtgcaaatg
     2041 cgcgtactcg atcaaatgga agaacgcccg cgcttagtta ctctggatac catgaatttt
     2101 tggatgaaca ccgccatnnn nnnnnnnaca gcctccgcga ggtgctgcgc cgcgtggatg
     2161 tgctcaccat caacgacgag gaggcgcgcc aactctccgg cgaacactcc ttggtaaaag
     2221 ccgccaaaat catccaccag atgggaccgc gttatttggt tatcaaaaaa ggagaacacg
     2281 gcgccctgtt gtttgaaggc gacagcctgt tttttgcacc ggcgctgccc ctcagcgaag
     2341 tggtggaccc caccggcgca ggcgatactt ttgccggcgg
//
LOCUS       DAORLF010000596         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24134_length_2378_cov_1.916487, whole genome shotgun sequence.
ACCESSION   DAORLF010000596 DAORLF010000000
VERSION     DAORLF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24134_length_2378_cov_1.916487"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1136)
                     /locus_tag="PK198_26550"
     CDS             complement(48..1136)
                     /locus_tag="PK198_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF42389.1"
                     /translation="MTEKLHKWRLILGDKADPDGQTMLGAEAQGMDRVLDALYDSDRQ
                     GSLGSSSPNINRWLGDIRRYFPVSVVQVMQRDALERLGLRQMLLQPELLEGMEPDVHL
                     AAMLLSLNKAMPAHTRETARQVVRKVAEAIEKRLAPALRQAVEGSIHKAVRNRRPKPH
                     EINWHRTIQANLRHFQPEYQTIIPQRLIGHGRKGRQLRHIILLADQSGSMSASIVYTG
                     VAAAVLASLRSVKTHLAVFDTAVVDLSEHLHDPVELLFATQLGGGTDINKALAYGQTL
                     VQHPADTILVLISDLFEGGNSRELLRRCAALKASGVQFIVLLALNDQGAPAYDHRIAA
                     EMAALGIPAFACTPGRFAEVMAEALNGQ"
     gene            complement(1269..1511)
                     /locus_tag="PK198_26555"
     CDS             complement(1269..1511)
                     /locus_tag="PK198_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRF42390.1"
                     /translation="MELLYFLLIGAAAGWLTGQLTKGKSFGIAGNIIIGVLGAVVGGF
                     LFGLLGISTSGGLFGQLITAVAGALVLLWVARFFRK"
     gene            1736..>2378
                     /locus_tag="PK198_26560"
     CDS             1736..>2378
                     /locus_tag="PK198_26560"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF42391.1"
                     /translation="MQPFIGREKELKRLEDIKKSARSEFVAVYGRRRIGKTMLIKQVF
                     ENDFVFHATAIANVTRQQQLLNFVTALRKYDPASAKEPVPTNWFFAFQQLTHYLERSA
                     PEKKIIFLDELPWFDNQKSDFVQSLEHFWNSWAAARQDVILIVCGSAAAWMVNTLINH
                     RGGLHNRVTEKIQVEPFQLQDAEKLLRLKSPDIDRYQMLQLYMVMGGVPFYLEA"
BASE COUNT          545 a          606 c          687 g          540 t
ORIGIN      
        1 ggtcagtgcg agactgaaaa ctgaatactg atctactgaa tactgatcta ctgtccattc
       61 aacgcctccg ccatcacctc ggcgaagcgg ccgggcgtgc aggcgaaggc gggaatgccc
      121 agcgcggcca tttcggcggc gatgcgatga tcgtaagcgg gcgcgccctg atcgttgagc
      181 gccaagagga caatgaactg taccccggaa gccttgagcg ctgcgcaacg gcgaaggagt
      241 tcgcgggaat tgccgccctc aaacaggtcg ctgatgagaa cgaggatggt gtcggcgggg
      301 tgttgtacca acgtctgccc ataggccagc gccttgttga tgtcggtgcc gccgcccaac
      361 tgcgtagcga agagcagttc taccggatcg tggaggtgct cactgaggtc aacaacggcg
      421 gtatcgaata cggccagatg cgttttcacg gagcgcaggg aggccagcac tgccgccgcc
      481 acgcctgtat aaacaatgga ggccgacata gagccgcttt gatcggccag caggatgatg
      541 tggcgcagtt ggcggccttt gcgtccgtgt ccgatgaggc gctgcggtat gatggtctgg
      601 tattcaggct gaaaatgccg caggttggcc tgtatggtgc ggtgccagtt gatttcgtgg
      661 ggtttggggc gacggttgcg caccgccttg tgaatactgc cctcaacggc ctggcggagc
      721 gccggcgcga ggcgcttctc aatagcttcg gctaccttgc gcaccacctg ccgggcagtt
      781 tcacgggtgt gggcgggcat ggctttgttg agcgacagga gcatggccgc gaggtgtaca
      841 tcgggttcca tgccttccaa tagttcgggc tgaaggagca tctggcgcaa gccgaggcgt
      901 tcgagcgcgt cgcgttgcat gacttgcacc acggaaacgg ggaagtaccg gcggatgtcg
      961 cccaaccagc gattgatatt gggggaagaa ctgccgaggc tgccctgccg gtcgctgtcg
     1021 tagagggcgt ccaagacgcg gtccatgccc tgggcttcag caccgagcat cgtttggcca
     1081 tcggggtcgg ctttgtcgcc gaggatgagc cgccatttat ggagtttttc ggtcatgttt
     1141 ttgcttgctg ttagctgctg gcctgatatt gcaagttgaa aagtctttac catgaaagtg
     1201 actttttgaa attttctggc ataaaaattt gattatctta cagttaccaa gctcgaagct
     1261 cgcggctatt attttctaaa aaacctcgcc acccacagca acacgagtgc gccggctacg
     1321 gcggtaatca actgtccgaa gaggccgccc gaagtgctga tgccgagcaa tccgaacagg
     1381 aagccgccta ccactgcgcc cagtacgccg atgatgatgt tgccggcaat gccgaaactt
     1441 ttgcctttgg tgagttggcc ggtgagccag cccgccgccg ctccgatgag caaaaagtag
     1501 agtaattcca tctgttgtag ttttcgaggc tgaaaagtag taatttactt gaaatatacg
     1561 gcgataaaaa gcggcttttg ttccgcaaac ggaggccgga caacagaggc ggcccgattt
     1621 tttttagata tttttggcgg taagtggggg caaaaagaca tttaccgtca gaaaataatt
     1681 ttatttttgg cggtaactcg gtttttaaac cacttaccgt caaaaaacaa cactcatgca
     1741 accctttatc ggcagagaaa aagagctaaa acggcttgag gatatcaaaa aaagcgctcg
     1801 ttctgagttc gttgcagtat atggcaggcg caggatcggc aagacgatgc tcatcaagca
     1861 ggtgttcgag aacgattttg tttttcacgc cactgccata gccaatgtta cccgtcagca
     1921 gcaattgctc aattttgtca cagcactgag aaaatacgat ccggcttccg ccaaggagcc
     1981 tgttccaacc aattggttct tcgcctttca gcagcttacc cattacctgg aacgctctgc
     2041 cccggagaaa aaaatcattt ttctggacga actaccgtgg tttgacaatc aaaaatccga
     2101 cttcgttcag tctctggagc atttctggaa cagttgggcg gcggcccggc aggatgtgat
     2161 tctgattgtt tgcggctctg cagcggcctg gatggtcaac acactcatca accacagagg
     2221 cggccttcat aaccgagtga cagagaagat acaggtagaa cccttccaac tacaagatgc
     2281 ggaaaaattg ctgcgcctga aaagccccga catagaccgg tatcagatgc ttcaactgta
     2341 catggtcatg ggaggtgttc ctttctatct ggaggccg
//
LOCUS       DAORLF010000597         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24150_length_2377_cov_2.036606, whole genome shotgun sequence.
ACCESSION   DAORLF010000597 DAORLF010000000
VERSION     DAORLF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24150_length_2377_cov_2.036606"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PK198_26565"
     CDS             <1..933
                     /locus_tag="PK198_26565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRF42392.1"
                     /translation="PLTISIVRSAANVAGMMLVYYVAIFLVDRYLEQRRHLVFGIFIT
                     LCILMAVVFRTQINLLFPSENLREIVITGDDVNNARFGALITSIGVALMAVLLRTRQL
                     RAIQEKRHWELLSKEREAQLRLLRSQINPHFLFNNLNNIYSLAVAGSDKTAPMLLRLS
                     QLLRYVTYETREALSPLASEIEHVQAYIELLRMSMEEPVPVGFSVEGQTNCRVEPAIL
                     LPIVENCFKHGDYHSNPKALIRFSLQVKAEGMLHFVAENSFDPQHVQKDQIGGVGLEN
                     IIKRLQLRFGESARLQTATIRDIFRVELEMPTSA"
     gene            988..1698
                     /locus_tag="PK198_26570"
     CDS             988..1698
                     /locus_tag="PK198_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF42393.1"
                     /translation="MESIKTLLLDDEYLALNLLENYVRQVEQLVVVGKYKSPVQALDV
                     LRTGEVDLLFLDIQMPGLSGLQLLSTLQRPPVTIFTTAYSEHALTAFNLNAVDYLLKP
                     FSFERFLQAVNKARTLLLPPAVKVDAYQAHKDFFSIKADGKLIKVAFDDILYIEGLKE
                     YVRIVCRDGRHVTLESLKNLEESILPAEFFLRVHKSYIVAKDKVQSVEGNVLHIGAHK
                     IPISRERRDEVVAAVFMI"
     gene            complement(1711..>2377)
                     /locus_tag="PK198_26575"
     CDS             complement(1711..>2377)
                     /locus_tag="PK198_26575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42394.1"
                     /translation="EAIALEVLKVRETSATQVAQAKESAAQEVAAAQKQAAQVIAEAR
                     TSADARRQEYARQIEEANTRATQQITEARDRSQRELDTLRNTVARQRTQYVQDVEAAR
                     KQSLEEVTEARKTASESVYQARQQATDQVTKVKSDAAVAIAAEQKKVEEARKQLATEK
                     ERIERETAALEAAEKQRIEAIRTESAKQEKQLMELKNQVKQAEEALKKLKEEMERQRN
                     GGN"
BASE COUNT          494 a          625 c          617 g          641 t
ORIGIN      
        1 cctctgacaa tcagcattgt acgcagtgcc gccaatgtag cgggcatgat gttggtgtac
       61 tatgtcgcca tatttttggt agatcgctat ttagaacagc ggcggcatct ggtgttcggc
      121 atattcataa cgctgtgcat cttgatggcc gtggtgtttc gcacgcaaat caaccttctg
      181 tttcccagcg aaaatctgcg cgagatcgtt atcaccggcg acgacgtcaa caacgcgcga
      241 tttggggcgc tcatcaccag cattggagtg gcgcttatgg ccgttttatt gcgcacccgg
      301 caattgaggg ccatacagga aaagcgccat tgggagctgc tgtcgaaaga gcgggaagcc
      361 cagctccgcc tgttgcgctc gcagatcaat ccacattttt tgttcaacaa cctcaacaac
      421 atctacagtc tggcagtagc cggctccgac aaaaccgcgc ctatgttatt gcgtctgagc
      481 cagttgttgc gatacgtcac ctacgaaacc cgcgaagcct tgtcgccgct ggcctccgaa
      541 atcgagcatg tgcaggctta cattgaactg cttcgcatga gcatggaaga accagtgccg
      601 gtgggcttct ccgttgaggg tcagaccaat tgtcgcgttg aacctgctat tttattacct
      661 atcgtagaga actgtttcaa acacggcgac taccactcca acccgaaggc gttgattcgc
      721 ttttctttgc aggtaaaagc ggagggaatg ctgcattttg tcgctgaaaa ttcttttgat
      781 ccgcagcatg tgcaaaagga ccagatagga ggagtgggcc tggagaatat tatcaaacgg
      841 ttgcaattgc gatttggaga atccgccagg ttgcaaactg ccaccatccg agatattttc
      901 agggtagaac ttgagatgcc gacgagtgcc tgatgcagcg gaataatatg tttttagaaa
      961 acctcttcac accaacattt cactcaaatg gaaagtataa aaacgctcct tctcgatgat
     1021 gaatatctgg cgctgaacct gttggagaat tatgtccggc aggtagagca actggtcgtt
     1081 gtgggcaaat acaaatcgcc ggtgcaggca ttggacgtac tccgaacggg cgaagtggac
     1141 ctgttgtttt tggacattca aatgccgggg ttgagcggtt tgcaattgct ttcaacgctg
     1201 cagcgcccac cggtaacgat ttttactacc gcatattcgg agcacgcgct gacggctttc
     1261 aacctcaatg ccgtggatta tctgctcaaa cccttttctt tcgagcgctt tttgcaggcc
     1321 gtcaacaaag cccgcactct gctgttgccg cctgccgtaa aagtggacgc gtatcaggcg
     1381 cacaaagatt ttttcagcat caaagctgac ggcaagctca tcaaggtggc ttttgacgac
     1441 atcctgtaca ttgaaggcct gaaagaatat gtgcgtattg tgtgccgaga cgggcggcac
     1501 gtcacattgg aaagcctgaa aaatctggag gagagcatcc tgcctgccga attttttttg
     1561 cgcgtacaca agtcttacat cgtggccaaa gacaaagtgc aatcggtaga gggcaatgtg
     1621 ttgcacatcg gcgcgcacaa aatccccatc agccgcgagc gccgcgatga ggtagtagcg
     1681 gctgtgttta tgatttgaga cagacggcgc tcaattcccc ccatttcgct gccgttccat
     1741 ttcctccttc agctttttga gcgcttcttc ggcctgtttc acctggtttt tcaattccat
     1801 caattgcttt tcctgtttgg ccgactcggt gcgaatggct tctattcgtt gcttttcggc
     1861 ggcttcgagg gcggcggttt cgcgctctat gcgttctttt tcagtggcca attgtttgcg
     1921 ggcctcttcc accttttttt gctcggcggc gatggcgacg gcggcatcag atttcacttt
     1981 cgtcacctga tccgtagctt gttgccgcgc ctgatacact gattcagagg ctgttttgcg
     2041 cgcttccgtc acttcttcca atgattgttt gcgtgccgct tccacatcct gcacatactg
     2101 ggtgcgctgg cgggccaccg tattgcgcag ggtatccaat tcgcgctgcg agcggtcgcg
     2161 ggcttctgtg atttgctgcg tggctctggt attcgcctct tctatttgcc tggcatattc
     2221 ttgccggcgc gcatccgccg aggtacgcgc ttccgcaatg acctgcgccg cctgtttttg
     2281 agcggcagcc acttcttgtg cagcgctttc tttggcctgt gctacctgtg tagccgaagt
     2341 ctcgcgcact ttcaacactt ccaatgcgat ggcttct
//
LOCUS       DAORLF010000598         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24206_length_2373_cov_2.436152, whole genome shotgun sequence.
ACCESSION   DAORLF010000598 DAORLF010000000
VERSION     DAORLF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24206_length_2373_cov_2.436152"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PK198_26580"
     CDS             complement(<1..919)
                     /locus_tag="PK198_26580"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF42395.1"
                     /translation="MKAITTLLAITLLSWATWLSAQNNCASGRYAEPVFTCVDVTPNI
                     TYGQGRQNWSTTNLLCQHGNLPPYNGFQTLLADLYEPCNDTETARPLIIAIHGGAWAT
                     GNKADMAPFGQAMARRGYVVASISYRLSITPNVLCFNAEVDRIKMVRAFYRAMQDAKA
                     AVRYFRANAAQYGIDPDKIFVGGHSAGAFTALGAGYLTDDSERPAETFVQNSCGNWLG
                     QLICQDLGGIEGEGGNPEVSSAVKGVIPMSGALTEMSYLDGPNDPPLLLVHGTADDVV
                     PYQSGCVLQNLGSLFTTCIPVFGSARVFTY"
     gene            complement(1046..>2373)
                     /locus_tag="PK198_26585"
     CDS             complement(1046..>2373)
                     /locus_tag="PK198_26585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42396.1"
                     /translation="ACNSYTFNGQTYTQSGIYTQMLTNAEGCDSLLVIELAIGQADTT
                     QFSATACNSYTFNGQTYTQSGIYTQMLTNAEGCDSLLVIELAIGQADTTQFSATACNS
                     YTFNGQTYTQSGIYTQMLTNAEGCDSLFTLHLEIVSPPALQFDVQHPSCADSGNGQAT
                     VAAAQGAPPFNYLWSNGQTTPVAIGLTTGPYTVTLTDANGCISIDTVTLTPQYQLLSS
                     FTVMGTPVCHGDSTAAVTVSPMGGAAPFEYHWLHGPQTASLSGLPAGIYHVTITDANG
                     CTATGATIFTDPPLLDISIVQNNHILTSQELDVEYQWINCDTGLPIQDAVNQSFEAIE
                     SGRYAVILSRGVCRDTSLCIEIMLVSAAEQRSALPKAEAYPNPNNGRFVLRMPVSGQI
                     ALYDATGRLLRTEVHGAGEHTLFIDGPAGLYWLAVMYNQGVQTLRIIRQ"
BASE COUNT          503 a          602 c          726 g          542 t
ORIGIN      
        1 cgtaagtaaa cactcgcgcg gagccgaaaa caggaatgca ggtagtgaac agcgacccca
       61 gattttgcaa tacgcagccc gactggtacg gcaccacgtc atcggcagtg ccgtgaacga
      121 gcagcagggg cgggtcgttg ggaccgtcga ggtaagacat ttcggtgagc gcgcccgaca
      181 tgggaatgac gcctttgact gccgaactga cctcggggtt gccgccctcg ccttcaatgc
      241 cgccgaggtc ctggcagatc aattggccga gccagttgcc gcaactgttt tgcacgaacg
      301 tttcggcggg gcgctcgctg tcgtcggtga ggtagcccgc gccgagggcc gtgaacgcac
      361 cggcactgtg gcctcccacg aatattttat cgggatcaat gccgtattgg gcggcgttgg
      421 cacgaaaata tcgcacggcg gctttagcgt cctgcatggc ccggtagaag gcgcgcacca
      481 ttttgatgcg atccacttct gcattgaaac acagtacatt gggtgtaatg ctgagtcggt
      541 agctgatgga ggccaccaca tagccgcgcc gtgccatggc ttgcccgaaa ggcgccatat
      601 cggctttgtt gcccgtagcc caggccccgc cgtgtatggc gatgatgagc gggcgggcgg
      661 tttcggtgtc gttgcagggt tcgtacaggt cggccagcag cgtttgaaag ccgttgtaag
      721 gcggcaggtt cccgtgctga cagaggaggt tggtggtgct ccagttttgg cggccttgcc
      781 cgtaagtgat attgggcgtc acatccacgc aagtgaacac cggctcggcg taccggcctg
      841 atgcgcagtt gttttgggcg gagagccaag tggcccacga caatagagtg atggcgagga
      901 gggtggtaat cgctttcata atgagactgt tagtgttttt aaacgacgaa tttcgctatg
      961 gcactttgct gcccgcattg cgccaggacg ctgtatattc ctttgccgaa ggtagaaata
     1021 tctatggaaa tgaaggatgt tttcctcatt gccgaatgat tcggagtgtc tgtaccccct
     1081 gattatacat aaccgccaac cagtacaagc ccgccgggcc atctataaaa agggtgtgct
     1141 cgccggcgcc gtggacttcg gttcgcagga gtcgcccggt ggcatcgtac agcgcaatct
     1201 gtccgcttac gggcattctg agtacaaacc ggccattgtt ggggttggga taagcctctg
     1261 cttttggcaa tgccgatctc tgctcagcgg cgctcaccaa catgatttca atacacagag
     1321 acgtgtcccg gcatacgccc cggctcagta ttacggcata tctgccgctt tcgatggcct
     1381 caaaagactg gtttacagca tcctgtatcg gtagaccggt atcgcagttg atccactgat
     1441 actccacgtc cagctcttgg gatgtaagaa tgtgattgtt ttgaacgatg gaaatatcca
     1501 gcaggggcgg atcggtaaag atggtggcac ctgtggcggt acagccgttc gcatctgtaa
     1561 tggtgacgtg gtaaatgccc gccggcagac cagacaaaga agcggtttga gggccgtgca
     1621 accaatggta ctcaaaagga gccgcaccgc ccatgggaga taccgtcaca gcggccgtgc
     1681 tgtcgccatg gcaaaccggc gttcccatta ccgtgaatga actcaatagc tggtactgcg
     1741 gagtcagggt aacggtatca atggaaatac aaccgttggc gtctgtcagc gtcaccgtgt
     1801 atgggccggt tgtcaggcca atggcgacag gtgttgtctg gccgttgctc cacagatagt
     1861 tgaatggagg ggcgccttgc gctgcggcga ctgttgcctg cccgttgccg gaatctgcac
     1921 aggaagggtg ctgtacatcg aactgcaacg caggcggcga tacgatttcc agatgcaaag
     1981 taaaaaggct gtcgcatccc tcggcattgg tcagcatttg agtgtaaata ccgctttggg
     2041 tataagtttg cccgttgaag gtgtaactgt tgcaggcggt ggcggaaaat tgggtggtat
     2101 cggcctgtcc tatcgccagc tcaataacga gaaggctgtc gcacccctcg gcattggtca
     2161 gcatttgagt ataaataccg ctttgggtat aagtttgccc gttgaaggtg taactgttgc
     2221 aggcggtggc ggaaaattgg gtggtatcgg cctgtcctat cgccagctca ataacgagaa
     2281 ggctgtcgca cccctcggca ttggtcagca tttgagtata aataccgctt tgggtataag
     2341 tttgcccgtt gaaggtgtaa ctgttgcagg cgg
//
LOCUS       DAORLF010000599         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24243_length_2370_cov_2.160259, whole genome shotgun sequence.
ACCESSION   DAORLF010000599 DAORLF010000000
VERSION     DAORLF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24243_length_2370_cov_2.160259"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>269)
                     /locus_tag="PK198_26590"
     CDS             complement(<1..>269)
                     /locus_tag="PK198_26590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42397.1"
                     /translation="GYDYFVYIVEPNDACIAPPRDKPEDVKAMRDQLTAHYAAYETSG
                     VVPPPFTGSGKTAPDKIKSDGDFRQALLAKLHSIRGSGAFAVSGV"
     assembly_gap    270..359
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(518..1102)
                     /locus_tag="PK198_26595"
     CDS             complement(518..1102)
                     /locus_tag="PK198_26595"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF42398.1"
                     /translation="MAITSLDQLDPNGTYTYADYLRWQFEERVELLRGKIRQMAAPSV
                     KHQRISIRFSRLLANALWQSPCQVFAAPFDVRLTRMRDDKEAKTVVQPDLCVICDPSK
                     LDERGCNGAPDLIIEILSPGNSRTEMKDKFELYQEAGVLEYWVVSPIEKTIQIWTLDE
                     QGFYISRRPKVEGDIATTPIIPNLEVEVAEVFAE"
     gene            1236..1934
                     /locus_tag="PK198_26600"
     CDS             1236..1934
                     /locus_tag="PK198_26600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF42399.1"
                     /translation="MKNYLIIGAASGIGAQLTAQLAAQGHRVFATWHHQAPFEAGPQV
                     SWHPLDVLDEQPDFGFLPDTLHGMAYCPGSISLKPFARIPESAFVQDYRLQVGGAVKC
                     IQAALPALKKAEQASIVLFSTVAVQAGFNFHTLVSASKGAVEGLTRALAAELAPKIRV
                     NAIAPSITDTPLAATLLNTEDKRAANAQRHPLRSIGKPEDIANTAEFLLSEKSGWMTG
                     QILHVDGGMSSLKV"
     gene            1941..2111
                     /locus_tag="PK198_26605"
     CDS             1941..2111
                     /locus_tag="PK198_26605"
                     /inference="COORDINATES: protein motif:HMM:NF021526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HRF42400.1"
                     /translation="MPKKQHLPQKTCPVCGRPFAWRKKWEKVWEEVKYCSDRCRKNKK
                     PELNLAGDEPKN"
     gene            2157..>2370
                     /locus_tag="PK198_26610"
     CDS             2157..>2370
                     /locus_tag="PK198_26610"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRF42401.1"
                     /translation="MTDENTSVHNPHDSFFKSLMHNRETALAFLEEFLPDEVKDVLDL
                     QSLELANTTFLTDELKQYFSDIVFKLK"
BASE COUNT          535 a          615 c          606 g          524 t
ORIGIN      
        1 acacgcctga aactgcaaaa gcgccgctgc ctctgatgct gtggagcttg gcgagcagtg
       61 cttgacggaa atctccatca gattttatct tgtccggtgc agtttttccg ctgcctgtaa
      121 agggcggcgg taccactccg cttgtttcgt aagctgcata atgggcagtc agttgatcgc
      181 gcatggcctt gacgtcctcc ggcttgtctc gcgggggcgc tatgcaggca tcattcggct
      241 caacgatata tacgaagtag tcgtagccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      361 aaactgagta cccgaccaca ccagccgctg atgtctgtgc cggtgtcttc gtctgtttgg
      421 ccggcgttga ttattacgga ttgtcctgtt ttgaacttca taatgtgctt tgaatgatca
      481 ttatcaagta gaaaagagtt gacgtatcag cgccgcctca ctcagcaaac acctccgcca
      541 cctctacttc caagttggga ataatcggcg tagtggcaat atctccttct acctttggcc
      601 gccgactgat gtaaaagccc tgctcgtcga gggtccaaat ctgaatggtt ttttcaatgg
      661 gcgagaccac ccagtattcc aggacgccgg cctcctgata gagttcaaac ttgtctttca
      721 tttcggtgcg gctattgccc ggcgagagga tttcgataat aaggtcgggt gctccgttac
      781 agcctcgttc gtccaatttg gaagggtcgc agatgacgca aaggtcgggt tgaacgactg
      841 ttttggcttc tttgtcgtcg cgcattctgg tgaggcgaac gtcgaaaggc gctgcaaaaa
      901 cctgacaagg gctttgccac aaggcattgg caagcaggcg ggaaaaccgg atagaaatgc
      961 gttgatgctt taccgagggc gcagccatct gccggatttt accgcgcagt aattccacgc
     1021 gctcttcaaa ctgccagcgc aaataatcgg cgtaggtgta tgtgccgtta ggatcgagtt
     1081 gatcgagcga agtgatggcc ataagcaata aataaactgg tttcggattc aaaggtacga
     1141 aaaacccgga gcctgtgcaa acgagtggtt atgacggcag gccctcaaaa accaaaatcc
     1201 ccctccgccg ttataccctc caaaaaacca accgcgtgaa aaattacctc atcatcggcg
     1261 cagcatcggg tatcggcgcc caactgaccg cgcaattggc ggcgcaaggc caccgcgttt
     1321 ttgccacctg gcaccatcaa gcacctttcg aggcggggcc gcaggtgtcg tggcatccat
     1381 tggacgtact cgacgagcag cccgacttcg gtttcctgcc cgataccctg cacggtatgg
     1441 cctattgccc cggcagcatc agcctcaagc ctttcgcccg catacctgag tcggcttttg
     1501 tgcaggatta ccgtttgcag gtgggcggcg cggtgaagtg catacaggcg gccctgcccg
     1561 cgctgaaaaa ggccgagcag gcttccatcg ttttgttttc cacggtggcg gtgcaggccg
     1621 gattcaattt tcataccttg gtgtcggctt ccaaaggcgc tgtggagggg cttacccgcg
     1681 cattggctgc cgagttggcg cccaaaatca gggtcaatgc gatagcgcca tccattaccg
     1741 atacgccctt agccgccacc ctgctcaata ccgaagacaa acgcgccgcc aacgcccaac
     1801 gccacccgct gcgcagcatc ggcaagccgg aagatattgc caacaccgcc gagtttctgc
     1861 tctctgaaaa atcgggctgg atgaccggcc agattttgca tgtggatggc ggcatgtcga
     1921 gccttaaagt gtaagctgac atgcccaaaa agcagcatct accccaaaag acctgcccgg
     1981 tatgcggccg ccctttcgcc tggcgcaaaa aatgggaaaa ggtgtgggag gaggtaaaat
     2041 attgcagcga tcggtgcagg aaaaataaaa agccggaact gaaccttgcc ggcgatgagc
     2101 cgaaaaattg agatgtcata tcgccgattc tggcatttta cattactttt gcccctatga
     2161 ccgacgagaa caccagcgta cacaatccgc acgacagctt cttcaagtct ctgatgcaca
     2221 atcgggagac ggcactcgct tttttggaag aatttctgcc agatgaagtg aaagatgtac
     2281 tggatttgca aagtctggaa ttggcgaata cgacctttct gaccgacgaa ctgaagcagt
     2341 atttttccga cattgttttc aaactgaaaa
//
LOCUS       DAORLF010000600         2363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24309_length_2363_cov_1.528163, whole genome shotgun sequence.
ACCESSION   DAORLF010000600 DAORLF010000000
VERSION     DAORLF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24309_length_2363_cov_1.528163"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..596
                     /locus_tag="PK198_26615"
     CDS             3..596
                     /locus_tag="PK198_26615"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRF42402.1"
                     /translation="MTNRNSTSDNCAMERLPRHFFLDKAFEEQAYEDNPFPIGNEQTI
                     SQPYTVAFQTEWLELRPRLKVLEIGTGSGYQAAVLAMMGARVYTVERYEALYVKAQEL
                     LHALGLKNVRCYFRDGNKGLPEHAPFDRIIATAGAVEVPEALRQQLAVGGMLVIPVGR
                     EVQRMLRVRRVSETEFVQEEQGDFKFVPFLGGTERRG"
     assembly_gap    679..875
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <876..>1395
                     /locus_tag="PK198_26620"
     CDS             <876..>1395
                     /locus_tag="PK198_26620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRF42403.1"
                     /translation="NTPITRADRASNELICSALLALTPDWPIISEENELPPYEARRAW
                     SYCWVVDPLDGTKEFIRRNGDFCVNIALVKDGRAVLGLVYAPVTGQCFWAVQGQGAFE
                     VTEAGERRLQCARFGMEDAGLRVLSSRSHFNTATQEWIGGLREPGFVNKGSAIKFGLL
                     ARGDAELYPRLGP"
     assembly_gap    1396..1412
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1555..>1867
                     /locus_tag="PK198_26625"
     CDS             1555..>1867
                     /locus_tag="PK198_26625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42404.1"
                     /translation="MSNQKPDTEQHGDFPQGLKGLAGALAQIDHQFVGVVCILFIMGG
                     MVASEAMMSMGMIFLAASAVFNLNFPDNMRHFFKHRALVALMLIFVVYFVSGLWSENT
                     AW"
     assembly_gap    1868..1877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1878..>2363
                     /locus_tag="PK198_26630"
     CDS             <1878..>2363
                     /locus_tag="PK198_26630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42405.1"
                     /translation="LDRVRMKLPFLLMPMAIVSIPRLDRQVYLKLLYAFFIWISLASL
                     HSLALYLADYEAINSAYREGRVLPTPVQHIRFSLMVAFCVAVGAYLLRERFVWKYRWE
                     RRLIQIVTIFLIVYLHILAVRSGLLALYAMMLYYAALLVWERKQYLLAGWLAVAMAVA
                     G"
BASE COUNT          403 a          489 c          741 g          506 t
ORIGIN      
        1 cgatgaccaa caggaattct acgagtgaca actgcgcgat ggagcggctg ccgaggcatt
       61 tttttttgga caaggctttt gaggagcagg cttatgaaga caaccccttc ccgataggca
      121 atgagcaaac gatatcgcag ccttatacgg tggcgtttca gacagagtgg ctggagttgc
      181 ggccgcgcct gaaggtgctg gaaataggca ccggatcggg gtatcaggcg gcggtgctgg
      241 ccatgatggg ggcgcgggtt tatacggtgg agcgctatga ggcgctgtat gtgaaggcgc
      301 aggaactgct gcatgcgctg gggctgaaga atgtgcgctg ctactttaga gacggcaaca
      361 aggggctgcc cgaacatgcg ccctttgaca ggatcatagc tacggcgggc gctgtggagg
      421 tgccggaggc gctgcggcag cagttggcgg tgggcggcat gttggtgatt ccggtgggcc
      481 gggaggtgca gcggatgttg cgggtgcggc gggtgtcgga aacggagttt gtgcaggaag
      541 agcagggcga ttttaaattt gtgccttttt tgggggggac ggagcggagg gggtagctgc
      601 ggtttgcggt gggcggtcat cggtaggcgg taggcggttt gcggtgggcg gttccgaccc
      661 gaaaccttga acccgcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacaac acccccatta cgcgtgcaga
      901 ccgggcctcc aatgagttga tatgcagtgc tttgttggca ttgacgcccg attggcccat
      961 catttctgag gagaatgaac tgccgcccta cgaagcgcgc cgcgcgtgga gctattgctg
     1021 ggttgtggac ccattagacg gcacgaagga attcatacgg cgcaatggcg atttttgcgt
     1081 caacatagca ttggtaaaag acgggcgcgc ggtgctgggc ttggtgtatg ctccggtgac
     1141 ggggcagtgc ttttgggccg tgcaggggca gggcgctttc gaggtgacgg aagcggggga
     1201 gcggcgtttg caatgtgcgc gttttgggat ggaagatgcc gggctgcgcg tgctcagttc
     1261 gcgttcgcat ttcaatacgg ccacgcagga atggataggc ggcttgcggg agccggggtt
     1321 tgtgaacaaa ggcagcgcca tcaaattcgg gctgctggcg cgcggcgatg ccgagctgta
     1381 cccgcggttg gggccnnnnn nnnnnnnnnn nncggctgcc ggccaggtga ttgtggagga
     1441 ggccggcggc gcagtgctgg atttttatac ccggcagccc ttgcgataca ataaagaaag
     1501 tacgctgaat ccgcatttta tagcgcttgg agcactcaaa tccgaaaagt aatcatgtcg
     1561 aatcagaaac ccgataccga acaacacggc gattttcctc aaggcttgaa gggcttggcc
     1621 ggcgctttgg cgcaaataga tcatcagttt gtgggggtgg tgtgcatcct gttcatcatg
     1681 ggcggcatgg tggcatcgga ggcgatgatg agcatgggca tgattttttt ggcggcgagt
     1741 gcggtgttca acctgaactt cccggacaac atgcgccact tcttcaaaca ccgggcattg
     1801 gtggcactca tgctgatttt tgtggtgtat tttgtgagcg gactgtggtc ggaaaacacc
     1861 gcgtggtnnn nnnnnnnggc tggaccgagt gcgcatgaaa ctgccgtttt tgctgatgcc
     1921 gatggccata gtttccatcc ctagattgga ccggcaggtg tatctgaagc tgctgtacgc
     1981 tttttttatc tggatttcat tggcctcgct gcattcattg gcgctttatc tggctgatta
     2041 tgaggccatc aactctgcat atcgcgaagg tcgggtattg cccacgccgg tacagcatat
     2101 ccgattcagt ctgatggtag ctttctgtgt ggcggtgggc gcgtacctgt tgcgcgagcg
     2161 ttttgtatgg aagtaccgtt gggagcgccg tctgatacaa atcgtcacca tttttttgat
     2221 cgtctatctg catatcctgg cagtgcgaag cggcctgttg gcactgtacg cgatgatgct
     2281 gtactacgcg gcgttgctgg tatgggagcg caaacaatac ctgctggccg ggtggctggc
     2341 agtggctatg gcggtggctg gtt
//
LOCUS       DAORLF010000601         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_24793_length_2328_cov_1.733392, whole genome shotgun sequence.
ACCESSION   DAORLF010000601 DAORLF010000000
VERSION     DAORLF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24793_length_2328_cov_1.733392"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(234..1070)
                     /locus_tag="PK198_26635"
     CDS             complement(234..1070)
                     /locus_tag="PK198_26635"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42406.1"
                     /translation="MNKRTLYTTTAVVTAILLWLANAAGPGNVQGIDRTGSPLSPGAC
                     DACHSGGNFAPTVTAELLDAGTPVTQYQPNKSYTLRVRVTAASGTPTRYGFQAVALTG
                     TTNTSAGTFGTAPTGFRKTTIVGRIYVEHSTPRTNTTMDFPWTSPATPGEEIRFYAAG
                     IAANNNSNSSGDAGAQLSAPLVLTPLVSSSNDIASEKFGLKILGNPIGDQLRIRMQAS
                     VSGEYRFALISLNGSTLWNREIYLAQGDHPLNFDASALPDGVYILQVSNGVNTTGIKL
                     IK"
     gene            1189..1839
                     /locus_tag="PK198_26640"
     CDS             1189..1839
                     /locus_tag="PK198_26640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42407.1"
                     /translation="MIQSCEIRWFLASAPEAVLIWFERRGQHFSKEEQAGRCDFYLVS
                     EAVRNDLGIKLREGNLEIKERTTEFGAHTFSASAEGRVEAWRKWSFKLAHTREGGTDE
                     VLHITRQDVQNSAWVPVGKDRLQVILEMAEESRVLLSGAPAFSLDEGCGVELTKIRLP
                     DRVCYSFALEAFSRTGHEYQNLKRAVDTMTAEIPGLELSLTESMGYPEFLNNIAKP"
     gene            1836..2249
                     /locus_tag="PK198_26645"
     CDS             1836..2249
                     /locus_tag="PK198_26645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42408.1"
                     /translation="MKALLLIISTTFLLQHPAELPDCDKSEAIMRIALNALLSREMIA
                     PHDNTLNIIVSEEWVRFLPAEIKTDRNWVQVLANQASVTDVILTNVLVNGANGAKVFF
                     RTSPIAGYQYSGTISLKCLDGAYVFDAIHYVSEIE"
BASE COUNT          604 a          519 c          645 g          550 t
ORIGIN      
        1 cgggaaccaa gcctttccag gaggcaatgc ccattttggg gaataaaaaa aagagaccga
       61 cgctcagtaa aagataagaa atgagaagaa taaaccaaaa tcccatgatg tcgctgttgt
      121 gttgatgagc gcgtaaagat aaagcaagat gtgaaaagcg gcgcaagaaa tatacttacg
      181 ccgcttttcc tattatgaac gaccttgaaa tcaggaagtt cnnnnnnnnn naattactta
      241 atcaacttga tgccggttgt attgacccca ttggaaacct gcaaaatata gactccatcg
      301 ggcagtgctg aagcatcgaa attcaatggg tgatcgcctt gagcaaggta tatttcccgg
      361 ttccacaaag tactgccgtt caatgatata agcgcaaaac gatactcgcc cgaaacagac
      421 gcctgcatac ggatgcgcag ttgatcgccg atgggattgc ccaatatttt caagccgaat
      481 ttttcggacg caatatcgtt ggagctgctc accaagggcg tcaacaccaa tggagcggag
      541 agttgagcgc cggcgtcgcc ggaagaatta ctgttgttgt tggcggcaat acctgctgca
      601 taaaaacgaa tctcttcacc cggcgtagcg ggcgatgtcc aggggaaatc catagtggta
      661 ttggtgcgcg gtgtgctgtg ctccacataa atgcgtccaa caatggtcgt ttttcggaaa
      721 ccggtggggg cagttccgaa agtacccgct gaggtattgg ttgtaccggt gagcgccact
      781 gcctgaaaac cgtaccttgt cggagtgccg ctggccgctg ttacccgcac gcgcagcgtg
      841 tagcttttgt tgggctggta ttgggtaacc ggtgttcctg catccagcaa ttcagccgtg
      901 acggtagggg caaaattccc cccgctatgg caggcatcac aggcgcccgg actcagcggc
      961 gagccggtgc ggtcaatgcc ttgcacgttt ccggggccgg cggcgttggc cagccaaagc
     1021 aagatggccg tcacaacggc agtagtagtg tagagtgttc ttttgttcat aaaaaggcaa
     1081 ttgttttgga aatagagtca aaaatgagac atgcgtttaa atggttgtga ataatttcta
     1141 tatttgacct gatccttgca aaaaaatgta ccaacacgaa acatgcgtat gatacaatcc
     1201 tgtgaaatac ggtggttttt agcatcagcg ccggaggccg tattgatttg gtttgaaagg
     1261 cggggccaac atttttccaa agaagagcag gcaggccgct gcgattttta tctggtatct
     1321 gaggcggtgc gcaatgattt gggcatcaag ctccgggagg gtaatctgga aatcaaagag
     1381 cgcacgacgg agttcggggc gcatacattc agcgcctcag cggagggtag ggtagaggcc
     1441 tggcgaaaat ggagcttcaa gttggctcat acgcgcgaag gcggcacgga cgaagtgctg
     1501 catatcacgc gacaggatgt gcagaacagc gcttgggttc cagtgggaaa agaccgtttg
     1561 caggtaatcc tggagatggc cgaggagagc cgggtgctgc tttcgggtgc gccggctttt
     1621 agtctggacg agggctgtgg ggtggagctg acgaaaattc gcctgcccga ccgcgtttgc
     1681 tattctttcg cgctggaggc attcagccgt accgggcatg aatatcagaa cctgaaacga
     1741 gctgtggaca cgatgacggc ggagataccg ggcttggagt tgtctttgac agagagtatg
     1801 ggatatcctg aatttttgaa taatattgcc aaaccatgaa ggcattgctt ctaataattt
     1861 caacaacgtt cttgctgcaa catcctgccg aactgccgga ctgcgataaa agcgaggcga
     1921 tcatgagaat agcactgaat gcactgcttt cgcgtgagat gatagccccc catgataaca
     1981 cattgaatat catagtttct gaagagtggg ttcgctttct tcccgccgaa atcaaaaccg
     2041 acagaaattg ggtgcaggtg cttgcaaacc aagcatcggt aaccgatgtg atacttacca
     2101 atgttttagt gaatggagcg aatggagcga aagtcttttt tcgaacatct cccatagcag
     2161 ggtatcaata ctcgggtact atctccttga aatgtttgga cggggcatac gtctttgacg
     2221 ccattcatta tgtgtctgag atagaataat tccccggggc gcagaaacat gatgttgatg
     2281 cctgcggcat accgctgatg caaaatcttc ttcgcatcac ggagcact
//
LOCUS       DAORLF010000602         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_25112_length_2304_cov_2.494442, whole genome shotgun sequence.
ACCESSION   DAORLF010000602 DAORLF010000000
VERSION     DAORLF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25112_length_2304_cov_2.494442"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK198_26650"
     CDS             complement(<1..545)
                     /locus_tag="PK198_26650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF42409.1"
                     /translation="MILSYPAFSQQPCQMFIRNLRSKSLAQLSDEQLMAGYRASGNPD
                     AVSELFGRYLHLVHAMCMKYADDPEDRSDLVMVIFEKVMTELTQSEVINFNTWLFSLS
                     RNACVSWFRQQQRYASNVENWGAGKKNEDWVVENEASERLYDEQDALETKLSAAIEQL
                     DERQRVCIHLFFFEKKSYKDIA"
     gene            complement(542..1609)
                     /locus_tag="PK198_26655"
     CDS             complement(542..1609)
                     /locus_tag="PK198_26655"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRF42410.1"
                     /translation="MKRIFFVLFGGLLLLNSCSEEAKKSFGGPNNAYGKLNDLVVICD
                     DMLWDGPIGDTIDYYYASAYPILPQPEPMYDLRHYDLKSIEMEPLLKELRTYLIVANL
                     KDEDSPVARMVRADLGEEKYQRAQSDPSFHTVVGKDKWARGQIIIYIFSFSEDGLAQA
                     LTENFSAISKRIRDSEHPHLKKRLFAGGEDFGLRDRILEKTGATLRVPKGYFMALDKE
                     EITWLRYETDKASSNILIQKLPYTSDTQLSKEGIKAIRNELGRKYVSSSTKGSYMLIN
                     DVDLPMFTYVKTIGNRYAIEARGIWELSNDFKGGPFVSYLIHNPDKNELLFIDGFVYA
                     PEEEKRNYVQYLDHVISGIKF"
     gene            complement(1732..1845)
                     /locus_tag="PK198_26660"
     CDS             complement(1732..1845)
                     /locus_tag="PK198_26660"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42411.1"
                     /translation="MEDVRAVAKYVLRHRIVRNYKAEAEGYSEERVIESLL"
     assembly_gap    1886..1901
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1902..2269)
                     /locus_tag="PK198_26665"
     CDS             complement(<1902..2269)
                     /locus_tag="PK198_26665"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF42412.1"
                     /translation="MPKPFFVLATQNPIEQEGTYPLPEAQLDRFMFNIPLDYPSYEEE
                     VQIVKSTTSSNQYDLRHVLTGQQILDFQQLVRKIPVADNVLEYAVSLVSKTRPNTPRA
                     TGMVNDYISWGAGPRASQYLV"
BASE COUNT          563 a          545 c          594 g          586 t
ORIGIN      
        1 gcaatgtctt tgtaactctt cttctcaaag aaaaacaaat gaatacacac cctttgacgc
       61 tcgtccaatt gctcaatggc ggcgctcaat tttgtttcca aagcatcctg ctcatcatat
      121 agtcgctcgg aggcttcatt ttccacaacc caatcttcat tttttttccc ggcgccccag
      181 ttttcgacgt tggaggcata gcgctgttgc tgtcgaaacc aggaaacaca agcatttcgg
      241 gagagtgaaa acagccaggt attgaaatta ataacttcac tctgagtgag ttcagtcatt
      301 actttttcaa aaatcaccat caccagatcg ctccggtctt cggggtcatc ggcatatttc
      361 atgcacatgg catggacgag gtgtagataa cggccaaaga gttcggaaac agcatcagga
      421 ttgccggacg cacgataccc cgccatgagt tgttcatcgc tgagttgggc aagagacttt
      481 gaacgaagat tccgaataaa catctggcat ggctgctgtg aaaacgccgg gtaagataag
      541 atcaaaattt gatacccgaa atcacatgat ccagatattg aacataattg cgtttttctt
      601 cttcgggagc atatacaaac ccgtcaatga aaagcagttc gtttttgtcg ggattatgga
      661 tgaggtaact gacgaaaggc ccgcccttga agtcgttgct cagctcccaa ataccgcgcg
      721 cttcaatggc gtaccggttt ccgatggttt tgacgtaggt gaacatcggc aagtccacat
      781 cattgatgag catataggac ccctttgtag aggaagacac atatttgcgc cccaattcat
      841 tgcgaatggc cttgatgccc tccttgctga gctgcgtgtc gctcgtgtac ggcagcttct
      901 gtatgagaat attgctggag gccttatcgg tttcatagcg gagccaggtg atttcctctt
      961 tgtccaaggc catgaaatac cctttgggca cgcgcagtgt ggcgccggtt ttttcgagta
     1021 tccggtcgcg tagtccaaag tcttcccctc ctgcaaacaa gcgttttttc aggtgcgggt
     1081 gctcgctgtc gcggatgcgc ttggagatgg cagaaaaatt ttcagtcagc gcctgtgcca
     1141 ggccgtcttc agaaaaagag aaaatgtaaa taatgatctg accgcgcgcc catttgtctt
     1201 tgcctactac ggtatggaaa gaaggatcgg attgggcgcg ttgatatttt tcctccccca
     1261 aatcggcccg aaccatgcgc gctacgggcg agtcttcatc cttcaggttg gccacgatga
     1321 gataagtgcg caactctttg agcagcggct ccatttcgat gcttttgagg tcataatggc
     1381 gcaggtcata catcggctcc ggctgcggca aaataggata agccgacgca taatagtaat
     1441 caatggtgtc gccgatgggg ccatcccaga gcatatcatc acaaatgact accagatcgt
     1501 tgagtttgcc gtaagcatta ttggggccgc caaatgattt tttagcctct tccgagcaac
     1561 tgttgagcaa aagcaagccg ccgaagagaa caaaaaaaat gcgtttcata ttattggttg
     1621 tttttgagct gtttacacgt caaaacgggg ttgccgtcgg gcaggcaaca ggctgaataa
     1681 aaccttagat gcaaacagga gtgattttgg cgggcagaag cgccggaaaa atcacaacaa
     1741 gctctcgatc acgcgttctt ccgagtagcc ttccgcctcg gccttgtaat tgcgcacaat
     1801 gcggtggcgc agcacatatt tggctacggc gcgtacatcc tcgatatccg gcgagtattt
     1861 tcctgcaata gcggcgtggc atttgnnnnn nnnnnnnnnn nacaagatat tgagaggcgc
     1921 gagggccggc gccccaggaa atgtagtcgt tcaccatgcc ggtagcacgc ggcgtgttgg
     1981 ggcgggtttt gctcaccaaa gagacggcgt attccagtac attgtcggca acgggtattt
     2041 tgcgcaccaa ttgctgaaaa tcaaggattt gctgtccggt gagtacatgg cggagatcgt
     2101 attgattgga agaagtagtg ctttttacga tctgcacttc ctcttcataa gaaggataat
     2161 cgagcgggat gttgaacatg aagcggtcga gttgcgcttc cggcagcggg taggtgcctt
     2221 cctgttcgat ggggttttga gtggccaata caaaaaacgg cttaggcaag gcgtgccgtt
     2281 ggccgctcac ggttaccgag cgct
//
LOCUS       DAORLF010000603         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_25246_length_2295_cov_1.758929, whole genome shotgun sequence.
ACCESSION   DAORLF010000603 DAORLF010000000
VERSION     DAORLF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25246_length_2295_cov_1.758929"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1010)
                     /locus_tag="PK198_26670"
     CDS             complement(54..>1010)
                     /locus_tag="PK198_26670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF42413.1"
                     /translation="PSGLNATNITASSATLNWGAVSGASSYNVRARQVGTTTWSTGTA
                     TGTSVNFTGLTASTQYEFQVQAVCGSTTGSYSGSANFTTLSSGGGGGCTETFEPNNSR
                     SGSLPLTAVNTNLSSQISTSTDQDWYRFANTSATRNIRVDLTTLPADYDVQLYQNNTL
                     RGTSQNDGTTSEVITWNTTTVASNWYVRVYGYNGAFSNTQCYTLRISLSSSSFRTDGS
                     TDGEVTEIEIPVEVINSGFSMFPNPALDQLTLDVNLEADRTVKVSVGDITGKTHILRS
                     FDLFKGNSRATLDVSHLPAGTYFVRVENGEVIGTQKLVIMRP"
     assembly_gap    1011..1212
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1213..>1429)
                     /locus_tag="PK198_26675"
     CDS             complement(<1213..>1429)
                     /locus_tag="PK198_26675"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF42414.1"
                     /translation="VVLHSSMGSVASPNPGGGSYARGRTLTHEVGHWLNLRHIWGDAT
                     CGNDQVADTPVHNTSNGGCPTYPHYSTC"
     assembly_gap    1430..1466
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1467..2046)
                     /locus_tag="PK198_26680"
     CDS             complement(<1467..2046)
                     /locus_tag="PK198_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42415.1"
                     /translation="MRLLATLLLSFFTLALFGQHDHRNCGAMEHLAEQLQKDPGMLQR
                     MEAIERHTEQYQRGDQVQFRAVITIPVVFHIVHNGDAIGSGENVSDAVVMAQLEQLNQ
                     DYRKLNSDAGLVPSIFQGVHADTEIQFCLAQRKPDGTATTGINRIQTTSASWTRTQIE
                     SNLKPSSIWDRNQYLNVWSVVFGGGDASLLGYA"
BASE COUNT          486 a          499 c          590 g          481 t
ORIGIN      
        1 ggccgcccct gcatacagag acggcccgtt tgagatgttg aaatatttgt ttattacggt
       61 ctcataatca ccagcttctg agtgccgatg acctcgccgt tttcaacgcg tacaaagtaa
      121 gtgcctgccg gcagatggct cacatcgagc gtggcgcggc tattgccttt gaacagatcg
      181 aagctgcgca ggatgtgcgt tttgccggtg atatcgccca cagacacctt caccgtgcgg
      241 tcggcttcca aattgacatc caaggtcaat tgatccaatg ccggattagg gaacatggaa
      301 aaaccagaat tgatgacctc caccggaatt tcgatttccg tcacctcgcc gtcggtgctg
      361 ccgtcggtac ggaaagacga actgctcagg ctgatgcgca gcgtgtagca ctgcgtattg
      421 ctgaaggcgc cgttgtagcc atacacccga acgtaccagt tggaagccac cgtggtggta
      481 ttccaggtga ttacttcgga agtagtcccg tcgttttgag aagtcccgcg aagtgtattg
      541 ttttgataca actgcacgtc ataatccgcc ggcaaagtgg tcaaatctac ccggatattg
      601 cgcgtagcgg aggtatttgc aaaacgatac cagtcttgat cggtagatgt agaaatctgc
      661 gaactgagat tggtattcac cgccgtcaag ggcagcgagc cgctgcggct gttgttgggt
      721 tcgaaggttt cggtacaacc gccgccgccg ccgctgctga gcgtggtaaa gttggccgaa
      781 ccggaatagc tgcccgtagt actgccgcat actgcctgca cctggaattc atactgcgtg
      841 gaggccgtca gaccggtaaa attgaccgag gtgccggtag ccgtgccggt actccaggta
      901 gtcgtaccca cctggcgggc gcggacattg taggaggagg cgccactcac tgcaccccag
      961 ttgagcgtag cgctgctggc ggtaatattg gtggcattca ggccggaagg nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nngcaggtgc tgtagtgcgg ataggtgggg caaccgccgt tggaagtgtt
     1261 gtgtaccggc gtatcggcta cctgatcgtt gccgcaggtg gcgtcgcccc agatgtggcg
     1321 aaggttgagc cagtggccta cctcgtgggt gagggtgcga ccgcgggcat agctaccgcc
     1381 gccgggattg gggctggcta ccgaacccat tgaggaatgc agcactactn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnngtgc atagccgagc aaacttgcat cgccgccgcc
     1501 aaaaacgacc gaccacacat tgagatactg gttgcgatcc caaatggagg agggcttgag
     1561 gttgctctcg atctgagtgc gcgtccagga agcagaagtc gtctggatgc gattgatgcc
     1621 ggtggtagcc gtgccgtcgg gcttgcgttg cgctaagcag aactggattt ccgtatcggc
     1681 atgtaccccc tgaaaaatgg agggaaccaa gcctgcatcg gaattgagtt tgcgatagtc
     1741 ttggttcagt tgctccaatt gagccattac cacagcatct gaaacgtttt cgcccgatcc
     1801 tatcgcatcg ccattgtgta caatgtggaa cactacgggg atggtaatca cggcgcggaa
     1861 ttgcacctga tcgccgcgct ggtactgctc ggtatggcgc tcaatggcct ccatgcgttg
     1921 cagcatgccc gggtcttttt gcaattgttc ggccaaatgt tccatggcgc cgcaattgcg
     1981 gtgatcgtgc tggccgaaga gggccaaagt aaagaaggaa agcagaagag ttgcaagtaa
     2041 acgcataatg tgttgttttg tgattatgga ataaaaaatc aaaagcgtgc aaatgtaaaa
     2101 aattatcaca attacacatg tcatcatttt tttttcataa aaatacgaat atcacaatat
     2161 atatttggcg ggtataacaa taaactctca agggtacaat ttgtgtacgg acaaaagcag
     2221 catttcggag ttatgctcgt ttaacggaag acttattagg ggcgataaaa aataaagtgg
     2281 tcattttcgc caaag
//
LOCUS       DAORLF010000604         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_25676_length_2265_cov_2.124434, whole genome shotgun sequence.
ACCESSION   DAORLF010000604 DAORLF010000000
VERSION     DAORLF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25676_length_2265_cov_2.124434"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..378)
                     /locus_tag="PK198_26685"
     CDS             complement(76..378)
                     /locus_tag="PK198_26685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42416.1"
                     /translation="MSKSIPRLRKHTQRGARQPNKAGSRKNDIGMEITGFCQIADQPE
                     PIFAQKILTKRRQRYAKTFKKYRLKFFFIFFPSSLAYMKTGALSLHSLSANELNVV"
     gene            415..1731
                     /locus_tag="PK198_26690"
     CDS             415..1731
                     /locus_tag="PK198_26690"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF42417.1"
                     /translation="MRKTFGVFLLLATVVFACTHEPFFSLDNEMEELIGDKNRYVLPD
                     SDDYSAIPQSPVNPLTREKVNLGKMLFFEPGFGVESMHAEGFKTFSCGSCHIPAAGFR
                     PNRMQGIADGGVGFGLKGEARDKYPGYAASEIDAQGARPLSVLNVAFVPNTMCNGSFG
                     AGGVNTGTEHRWGVADPGTAINHEHLGALEGQNIEGLKVHRLLYNRELVEANGYKAMF
                     DAAFPDVPEADRYTRKTASFAISAYLRTLLTNQAPFQRWLKGDKGAMTEEQKRGAVLF
                     FGKAGCVRCHNEPNLGSMTFHALGVEDLYQNGGLKTSEADLRNLGRGGFTGNPTDLYK
                     FRVPQLYNLGDSGPYFHGSSKETLEEVIAYFNAGVPENGNVPQGQISAFFHPLGMDQA
                     EMADLTAFIRYSLRDPNLQRYVPERIMSGLCFPNNDPLSRFQMGCN"
     gene            1833..2123
                     /locus_tag="PK198_26695"
     CDS             1833..2123
                     /locus_tag="PK198_26695"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF42418.1"
                     /translation="MSLATLINHPEATLVDVRESWEFSMGHVEGSINIPLGQVFARID
                     EFRNMSKPLVLFCVSGNRSGMAEAFLKAQGVADVHNGGAWQDVAYYRSKKVA"
BASE COUNT          541 a          566 c          617 g          541 t
ORIGIN      
        1 gaccggacgg gactcgaacc cgcgacctcc gccgtgacag ggcggcattc taaccgactg
       61 aactaccgat cctttttaaa caacattcag ctcgtttgcc gaaagcgagt gcaaagataa
      121 tgcacccgtt ttcatatatg caagtgatga aggaaaaaaa atgaaaaaaa atttgagcct
      181 gtactttttg aaagttttgg cgtatctttg ccgacgcttt gtgagaattt tttgtgcaaa
      241 gatcggctca ggctgatcag caatttggca aaacccggtt atctccatac caatatcgtt
      301 ttttctgctg cccgctttgt tgggttgtcg tgctccccgc tgtgtgtgtt tccgcagccg
      361 gggaatggat ttggacattt tttaatccta taaacatctg aaaaacaaat tgttatgcga
      421 aagacttttg gggtgttttt attgttggca acagtggtgt ttgcctgcac ccatgaacct
      481 tttttctctt tggacaacga aatggaagaa ctgatcggcg ataagaatcg ctatgtcttg
      541 cccgacagcg acgactacag tgccattccg cagtcacccg tcaacccttt gacgcgggaa
      601 aaagtaaacc tgggcaagat gctctttttt gagcctgggt tcggcgtaga gtctatgcac
      661 gccgaagggt ttaaaacctt ctcctgcggc tcttgccaca ttccggctgc gggtttcagg
      721 cccaaccgaa tgcagggcat cgccgacgga ggcgtgggct ttggcctcaa gggggaagcg
      781 cgcgataagt atccgggcta tgctgcttcg gagattgacg cacagggcgc tcgcccgctc
      841 agtgtgctca atgtcgcatt tgtgcctaac accatgtgta acggctcgtt cggggcgggc
      901 ggagtcaata ccggcaccga acaccgctgg ggcgtagccg atccgggtac agccatcaac
      961 cacgaacatc tgggcgcgtt ggaaggccag aacatagaag gcttgaaggt acaccgtttg
     1021 ctctacaaca gagaattagt ggaagccaac ggctataaag ccatgttcga tgcggctttc
     1081 cctgatgtgc cggaagcaga ccgctatacc cgcaagaccg ccagttttgc catttcggcc
     1141 tacctccgca ccttattgac caatcaggcg ccgttccaac gttggctcaa aggcgataaa
     1201 ggcgccatga cagaggagca gaagcgcggc gcggttttgt ttttcggcaa ggcgggctgc
     1261 gtgcgttgtc acaatgaacc caacctcggc tctatgacat tccatgcgct gggcgtggaa
     1321 gatttgtacc aaaacggcgg actgaaaaca tcggaggccg atctgcgcaa tctcggcagg
     1381 ggtggtttca cgggcaatcc gactgatctg tacaaattca gagtgccgca gttgtacaat
     1441 ctcggcgatt cggggccgta tttccatggc agcagcaaag aaacgctgga ggaggtgata
     1501 gcttacttca acgccggtgt tcctgaaaac ggcaacgtgc ctcaggggca aatctctgca
     1561 tttttccatc cgctgggtat ggatcaggct gaaatggcag acctcacggc atttatccgt
     1621 tacagtctgc gcgacccgaa tctacagcgc tatgtcccgg agcggatcat gtcggggctt
     1681 tgcttcccca ataacgaccc gctttcgagg ttccagatgg gatgcaattg agaaaaaaca
     1741 gattgtgaca atcattactg acggggtgaa cgcggcggcc ctacttttgc gccatcattt
     1801 tatcaccaag ttaaaaaaaa cgattgtcac aaatgagttt agccactttg atcaaccatc
     1861 ctgaagccac gctggtggac gttcgcgaaa gctgggaatt cagcatgggc catgtagaag
     1921 gctccatcaa cattccgctg gggcaggttt ttgcacggat tgatgaattc cgcaacatgt
     1981 cgaaaccgtt ggttttgttt tgcgtgtcgg gcaaccgcag cggtatggcc gaagctttcc
     2041 tcaaagcgca gggagtagcc gatgttcaca acggcggcgc ctggcaggac gtggcctact
     2101 atcgctcgaa gaaagtagcc tgacataaat aacaggtgtg ctgtgagcgc tcaaaagctg
     2161 cgagccacga gccgcgagct acgagatgca atgtaaatac actggcgctt gttttgttat
     2221 gccagatgca ggccgtaagc tgaaagggtt tacggcctgt gttgt
//
LOCUS       DAORLF010000605         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_25968_length_2246_cov_2.398448, whole genome shotgun sequence.
ACCESSION   DAORLF010000605 DAORLF010000000
VERSION     DAORLF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25968_length_2246_cov_2.398448"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..784)
                     /locus_tag="PK198_26700"
     CDS             complement(65..784)
                     /locus_tag="PK198_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRF42419.1"
                     /translation="MGRAFEYRKARKMKRWAGMAKTFTRIGREIAIAIKDGVPDPAYN
                     SRLRMAIQNAKAANMPKANVENAIKKATAKDAENYDEVLYEGYAPHGIAVLVETATNN
                     TTRTVANVRHVFSKYGGSLGVSGSVDYMFTRKAVFRVLKDNHNLEELELELIDHGLSE
                     LKEEDDQLALYCDFVDFGALQKGLEEKGMEVQSSELIRIPGHYVKITEEQVEDVIKLI
                     EKMEEDDDVTNVFHNMDMGEE"
     gene            866..>2246
                     /locus_tag="PK198_26705"
     CDS             866..>2246
                     /locus_tag="PK198_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF42420.1"
                     /translation="MLKRLLFLFPALFGLLQPIFSQEKEPKWDVNNPPGPFKEVEFSL
                     TEGTWMNLDVSPDGKTIAFDLLGDIYTMPIGGGQAKVLRQGLAWEVQPRFSPDGKKIL
                     FTSDAGGGDNIWTMNADGSGAQQVTKENFRLLNNAAWMPDGQYIVARKHFTSSRSLGA
                     GEMWLYHISGGEGLQLTKRKNDQQDVNEPIVSPDGRYVYFSEDMYPGGYFQYNKDPNS
                     QIFVIRRYDRQKGEIEDIVAGPGGACRPQISNDGKHLAFVRRVRTQSVLFIHNLETGE
                     EMPVWDGLDKDQQEAWTIFGIYPGFDWMPDDKHIVVWGQGKIWKVNVASKNAEQIPFT
                     VQAKHRFQETVRFENKVFSDEFGVKVIRHARTSPDGKLLVFNAAGYLWKKQLPDGTPV
                     RLTQSQEFEYEPAFSPDGKEIVYVSWTDADMGALRKLNLATGQSAKLTTEKGIYRTPN
                     FSPDGKTIVY"
BASE COUNT          515 a          638 c          566 g          527 t
ORIGIN      
        1 actcacccct cacaaactca cccctcacaa actcacccct cacaaactca cccctaaaag
       61 aaccctactc ctcgcccata tccatattgt ggaaaacgtt ggtcacgtcg tcgtcttctt
      121 ccatcttttc gatgagtttg atgacatctt ctacctgttc ctcggtgatt ttgacgtaat
      181 ggccgggaat gcggatcaat tcggagcttt gcacctccat gcccttttct tccaagcctt
      241 tttgcagtgc gccgaaatca acgaaatcgc agtacagcgc cagttgatcg tcttcttctt
      301 tgagttcaga aagcccgtga tcgatcagtt cgagttctaa ttcttccaga ttgtggttgt
      361 ctttgaggac gcggaaaacc gctttgcgcg tgaacatata gtccaccgaa ccggaaacgc
      421 ccagtgagcc gccgtatttg gaaaaaacat ggcgtacatt ggctacggtg cgggtcgtgt
      481 tgttggtcgc cgtttccacc aataccgcaa tgccgtgcgg ggcatagcct tcgtagagta
      541 cctcatcgta gttttcggcg tctttggccg ttgctttttt gatggcattt tccacattgg
      601 ccttgggcat gttggctgcc ttggcgtttt ggatggccat gcgcaggcgg gagttgtaag
      661 cgggatcggg cacgccgtct ttaatggcga tggcaatttc ccggccgatg cgggtgaagg
      721 ttttggccat gccggcccag cgcttcattt ttctggcttt gcgatattca aatgcgcgtc
      781 ccatgtggtg tgtgcttttt tttgaggcgc aaaaatagcg ttgttttttc tacttttgcc
      841 acccaaccaa aaaataagat gcaccatgct gaagcgactg ttgtttctgt tcccggccct
      901 gtttggcctc cttcaaccga ttttttctca ggaaaaagaa cccaagtggg atgtcaacaa
      961 cccgccgggg ccgttcaaag aggtagaatt cagcctcacc gaaggcacct ggatgaatct
     1021 ggacgtcagt cccgacggca aaaccatcgc atttgacctg ctgggcgata tttataccat
     1081 gcccatcggc ggcggccagg ccaaagtgtt gcgccagggc ttagcctggg aagtgcagcc
     1141 gcgtttcagc cccgacggca aaaaaatcct cttcaccagc gatgccggcg gcggcgacaa
     1201 tatctggacg atgaacgccg acggatcggg cgcgcaacag gtgaccaaag agaacttccg
     1261 cctgctcaac aatgccgcct ggatgcctga tggtcagtac attgtggcgc gcaagcactt
     1321 tacttcctcc cgctcgctgg gcgccggcga aatgtggctc tatcacatca gtggcggcga
     1381 agggctgcaa ctcaccaaac gcaaaaacga ccagcaggat gtgaacgaac ccattgtttc
     1441 gcccgatggc cgctatgtgt atttcagcga agacatgtac cccggcggct attttcagta
     1501 caacaaagac cccaacagcc agattttcgt catccgccgc tacgaccgcc aaaaaggcga
     1561 aattgaagac atcgtggccg gtcccggcgg cgcttgtcgc ccgcaaatct ccaacgacgg
     1621 caagcatttg gcctttgtgc gccgcgttcg cacgcaatcc gtcctgttca ttcacaactt
     1681 agaaaccggc gaggaaatgc ccgtttggga cggcttggac aaagaccaac aggaagcctg
     1741 gaccatcttc ggcatctatc ccggtttcga ctggatgccc gacgataagc acatcgtagt
     1801 gtggggccag ggcaaaatct ggaaggtaaa tgtggcttca aaaaatgccg aacaaattcc
     1861 cttcaccgtg caggccaagc accgttttca ggagaccgtg cgctttgaaa acaaggtctt
     1921 cagcgatgaa tttggggtga aagtcattcg ccatgcccgc acctcacccg atggtaagtt
     1981 gttggttttc aacgctgccg gttatctttg gaaaaaacaa ttgcccgatg gaacgcccgt
     2041 tcggctcacc caaagccagg aatttgagta cgaaccggcc ttctcgcccg acggcaaaga
     2101 aatcgtttat gtaagctgga ccgacgccga catgggcgcc ctgcgcaagc tcaacctcgc
     2161 taccggccaa tcggcaaaac tcaccactga aaagggcatt tatcgcacgc cgaacttctc
     2221 gcccgacggc aaaaccatcg tatatc
//
LOCUS       DAORLF010000606         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_26239_length_2227_cov_1.629834, whole genome shotgun sequence.
ACCESSION   DAORLF010000606 DAORLF010000000
VERSION     DAORLF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26239_length_2227_cov_1.629834"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>391)
                     /locus_tag="PK198_26710"
     CDS             complement(5..>391)
                     /locus_tag="PK198_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRF42421.1"
                     /translation="YKPFAARYAVGEQSNFIHVPMMLTAIRQILDWGVENVQSYCKQI
                     SEEAISKIVAMGARIDTSHQRAAHLFGVRLGDAFDAEKLQAALHEHKVYVSFRGDAIR
                     VAPHVYNTKADFATLVACFEKSSLMR"
     assembly_gap    392..448
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<449..1174)
                     /locus_tag="PK198_26715"
     CDS             complement(<449..1174)
                     /locus_tag="PK198_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF42422.1"
                     /translation="MLDCQKHLFDLEDEVVYLNGAYMSPQLKSVEAAGLQGLRMKNRP
                     YQVSIQDFFQPVEDLKVAFAQLIHATEYQRVAVIPSVSYGIANAARNLPVSKGQKILL
                     MQEQFPSNYYIWEKVALEKGARLEIISAPSAPERGEAWTEKILQSIDNETAVVAMAHV
                     HWADGSVFDLKRVGEKARKMGAALVIDGTQSVGALPFDVREIQPDALICAGYKWLLGP
                     YSIGLAYYGPAFDNGRPIEENWIN"
     gene            complement(1183..1713)
                     /locus_tag="PK198_26720"
     CDS             complement(1183..1713)
                     /locus_tag="PK198_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF42423.1"
                     /translation="MRISHFLTAALLLLCTPSVFAQKAGTPEAKLAELGITLPETGAP
                     VANYVNAVRTGKLIFLAGKGPRQPDGKYITGQVGRDLTVQEGYEAARLTAINQLAALK
                     AEIGDLSRVKRIVKVLGMVNAGDGFTQHPQVVNGFSDLMVAVFGDKGKHARSAVGMSS
                     LPLGMCVEVEMIVEIK"
     gene            complement(1724..>1958)
                     /locus_tag="PK198_26725"
     CDS             complement(1724..>1958)
                     /locus_tag="PK198_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF42424.1"
                     /translation="LGRDNIILTRAVTGAEDFSFFAKEVPGLFLFVGGMTPGQDPTTA
                     APHHTPDFIINEAGMLHGVKLYCNLALDYLKRH"
     assembly_gap    1959..1969
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1970..>2227)
                     /locus_tag="PK198_26730"
     CDS             complement(<1970..>2227)
                     /locus_tag="PK198_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRF42425.1"
                     /translation="ELTKEAAVISVGRIQGGVRNNIIPEEVEMEGTIRTLETGMQDKL
                     HADIHRTATNIAESMGATAEVEIFKYVPVTYNDPKLTDEMLP"
BASE COUNT          501 a          607 c          515 g          536 t
ORIGIN      
        1 tcgctcacct catcaacgac gacttttcaa aacacgctac gagcgtcgca aaatccgctt
       61 tggtattata aacatgcggc gctacccgaa tggcatctcc acgaaacgag acatatactt
      121 tgtgttcatg gagcgcagct tgcagctttt ccgcatcaaa agcatctccc aaacggacgc
      181 caaacaagtg agccgcacgc tgatgagacg tgtctattct tgcgcccatt gccactattt
      241 tacttatagc ttcttcacta atttgcttgc agtacgattg gacattttct acgccccagt
      301 cgagtatctg acggatagct gtcagcatca tcggcacatg tataaaattg ctttgttccc
      361 caacggcgta acgcgccgca aaaggcttgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tgatccagtt ttcctctatc gggcgcccat
      481 tatcgaaggc cggcccgtaa tacgccaatc cgatggaata cggccccaac aaccacttgt
      541 agccggcgca aataagcgcg tcgggctgaa tttcccgtac atcaaaaggc aacgcgccaa
      601 cagactgagt gccgtcaatg acaagggcag cacccatttt tcgggccttc tcccctaccc
      661 ttttcaggtc aaaaacactg ccgtcggccc aatggacatg cgccattgcg acgacagccg
      721 tctcattatc aatactttga aggatttttt cggtccacgc ctcccctctt tcgggggctg
      781 acggggcgga aataatctca agtctcgcgc ctttttccaa ggccaccttc tcccagatgt
      841 aataattgct gggaaactgc tcctgcatca gcagtatctt ttgccctttg gaaacaggca
      901 aattccgggc ggcgttggct atgccgtaag atacagaagg tataactgca actctttgat
      961 attcagtggc atgaatcagt tgagcaaaag cgacttttaa atcctccact ggctgaaaaa
     1021 agtcctgaat actgacctgg tacggtcggt ttttcattct aagcccctgc aatccggcag
     1081 cttctaccga tttcaactgc ggcgacatgt aagcgccatt gagataaacg acctcatctt
     1141 cgagatcgaa caggtgtttt tggcaatcga gcacagcctt gattatttga tttctacgat
     1201 catttccact tccacacaca tgcccaaagg cagggaggac atccctacgg cagagcgtgc
     1261 gtgtttgcct ttgtcgccga acaccgccac catcagatcg gaaaaaccgt tgactacctg
     1321 cggatgctgt gtgaaaccat ctccggcatt caccatgccc aataccttca caatacgttt
     1381 gacccggctc aaatcgccga tttcagcctt gagcgccgcc aattggttga tggccgtcag
     1441 tctggcagcc tcgtagccct cctgcacagt cagatcgcgc cccacctgcc cagtgatgta
     1501 cttcccatcg ggttgccggg ggcctttgcc tgccagaaag atcagtttgc ccgttcgtac
     1561 cgcattgacg taattggcga ccggcgctcc ggtttccggc aaagtaatgc ccaactccgc
     1621 cagttttgct tcgggcgtgc ctgctttttg ggcaaacaca gacggcgtac aaagcagtag
     1681 caaggccgct gtgaggaaat gggaaatgcg cataattgtc cgattaatgt cttttcaaat
     1741 aatccagcgc gagattgcaa tacaacttaa cgccgtgcaa catacccgcc tcatttataa
     1801 tgaaatcggg cgtatgatga ggtgctgctg tagtggggtc ttggccgggt gtcatccccc
     1861 ctacaaagag gaaaagaccg ggcacttctt tggcaaaaaa ggagaagtct tcggcgccgg
     1921 tgacggcacg ggtcagaatg atattgtcgc gccccagcnn nnnnnnnnng ggcagcattt
     1981 cgtccgtcag tttaggatca ttgtaggtca cgggcacata tttgaaaatc tccacctcgg
     2041 cagtggcgcc catgctctcg gcaatgttgg tagctgtgcg gtgaatgtcg gcgtggagtt
     2101 tgtcctgcat accggtttcc agcgtacgga tggtaccttc catttctacc tcttcgggaa
     2161 tgatgttgtt gcgcaccccg ccctgaatgc gacctacgct gatgaccgcc gcttctttgg
     2221 tcagttc
//
LOCUS       DAORLF010000607         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_26712_length_2197_cov_2.062092, whole genome shotgun sequence.
ACCESSION   DAORLF010000607 DAORLF010000000
VERSION     DAORLF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26712_length_2197_cov_2.062092"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2197
                     /locus_tag="PK198_26735"
     CDS             <1..>2197
                     /locus_tag="PK198_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRF42426.1"
                     /translation="VGGGSYRVFAMPLESPIHGARTLVTDPADAIASPYGWHDTNGAP
                     GAEFTITRGNNVHAALDLSAPNGADAGSEPNGGAELVFDFPFDPNAAPDTYRPAAVTN
                     LFFWNNLVHDFAYKYGFTEAAGNFQVNNYGRGGTANDDVRAEGQDYSGTNNANFATPS
                     DGSRPRMQMFVWNQGLSSVTVNSPAPAVYNNIGNAGFGPLNFNMSGDLELSNPIDACA
                     APGASIAGKIAVIDRGNCTFVDKVLAAQSAGAIGVIICNNAAGDPITMGGTGAVTIPS
                     LMMTDVDCFALKMLMQSGTVNVSLSRVLKQYDSDFDNGVIAHEYGHGISNRLTGGRTN
                     VNCLGNQEQMGEGWSDWYGLMMTLKPGDSPTTPRGIGTYVSGQTTFGGGIRPTRYSTD
                     MSVNTATYNTIKTVSIPHGVGYVWCGMLWDLTWAMIEDHGFATGFDVAMWLVNDGMKL
                     QPCSPGFVTGRDAILAADQARYGGANSCRIWEVFARRGLGYSASQGSSNNVMDGIEAY
                     DLPDYDNDGYSCKEDCDNFNAAINPGAFEACDGIDNNCSGQIDEAGPPPSPWTTANVG
                     FANGNTVYTPCPAVNPTFTVQASGNSVQSADTEHLVSRMICGTNASITVKVSSLNGGG
                     WAGITMRESAAPGSKMVALKTQLGNNVIREVRATANGFKQSQSSPSNPANVWLRINRI
                     GNSFQFYVSTNGTTWQLTGSTTVVMNNCLMVGMFAESINANATTNAAFSN"
BASE COUNT          510 a          616 c          581 g          490 t
ORIGIN      
        1 agtaggcggc gggtcctatc gcgtttttgc catgcctttg gaaagcccca ttcacggtgc
       61 gcgtactttg gtaaccgatc ctgccgacgc cattgcctct ccctacggtt ggcacgatac
      121 caacggcgct ccgggcgctg aatttaccat cactcgtggc aataacgtac atgcggccct
      181 cgatttgagt gcgcccaatg gcgctgatgc aggctcagag cctaatggcg gcgccgaatt
      241 ggtattcgat tttccattcg acccaaatgc cgcacccgac acttaccgcc cggcagcagt
      301 gacgaatctt ttcttctgga acaatctggt acacgatttt gcctataaat acggctttac
      361 ggaagcagcg ggcaattttc aggtcaataa ttatggtcgc ggaggtaccg ccaatgacga
      421 tgtaagagcc gaaggacaag actacagcgg taccaacaat gccaactttg ccacaccgtc
      481 agacggcagc cgtccgcgta tgcaaatgtt tgtctggaat caggggctga gcagtgtcac
      541 ggtcaattcg cccgcgcctg ccgtttacaa caacatcggc aatgcaggct tcggcccgct
      601 caatttcaat atgagcggcg atctggaatt gtccaatcca atagatgcct gtgcagcccc
      661 gggcgcctcc atcgccggca aaattgctgt cattgatcgc ggcaattgta cttttgttga
      721 taaagtgctg gctgctcaaa gcgccggcgc tattggtgtg attatctgca acaatgcagc
      781 cggagacccc atcactatgg gcggtaccgg ggcggtcacc atcccttctt tgatgatgac
      841 cgacgttgac tgctttgcgc ttaaaatgct catgcagtca ggtactgtga atgtaagttt
      901 gagcagggtt ttgaagcaat acgattccga cttcgacaac ggtgttattg cacacgaata
      961 cggtcatggt atttccaacc gtctcaccgg cggccgcacc aatgtgaact gccttggcaa
     1021 tcaggaacaa atgggtgaag gctggagcga ctggtacggc ctgatgatga ccctcaagcc
     1081 cggcgacagc cctaccacgc cacgcggcat aggtacttat gtgtctggcc aaacaacttt
     1141 cggcggcggc atacgcccca cgcgctactc tactgatatg tctgtcaata cggccactta
     1201 caacaccatc aagacggttt ctatacctca tggcgtaggt tatgtatggt gcggcatgtt
     1261 gtgggacctc acctgggcta tgattgaaga tcacggtttt gctaccggtt ttgacgtggc
     1321 catgtggttg gtgaatgacg gtatgaagtt gcagccttgc agccctggct tcgtcaccgg
     1381 cagagacgcc attcttgctg ccgatcaggc acgctacggc ggcgccaaca gttgccgtat
     1441 atgggaggta tttgcccgtc gcggcttggg ctacagcgca tcacaaggca gcagcaacaa
     1501 cgttatggac ggcattgaag cctatgacct gcccgactat gacaatgacg gatattcctg
     1561 caaagaggat tgcgacaatt ttaatgccgc catcaatccc ggcgcttttg aagcctgcga
     1621 cggcatagac aacaattgca gcggccagat agacgaagcc ggcccgccgc caagcccctg
     1681 gacgactgcc aacgtaggtt ttgccaatgg caatacggtt tacacgccct gcccggcagt
     1741 gaatccgacc ttcacggttc aggcctccgg caattccgtt cagagcgccg acacggagca
     1801 tttggtcagc cgcatgatct gtggtaccaa tgcaagcatc actgtgaaag tcagcagctt
     1861 gaacggtggt ggctgggcag gcatcaccat gcgtgaaagc gctgcacccg gctctaaaat
     1921 ggtggctctg aaaacgcagc tcggcaacaa cgtcatcaga gaagtacgcg ccacggccaa
     1981 cgggttcaag cagtcccaga gcagcccgtc caaccctgca aatgtctggt tgcgcatcaa
     2041 tcgcatcggc aacagcttcc agttctatgt ttctaccaac ggaaccacct ggcaattgac
     2101 aggcagcact acggtcgtta tgaacaactg cctcatggtg ggtatgtttg cagaaagcat
     2161 caacgccaat gcgactacca atgctgcatt cagcaat
//
LOCUS       DAORLF010000608         2192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_26782_length_2192_cov_2.026205, whole genome shotgun sequence.
ACCESSION   DAORLF010000608 DAORLF010000000
VERSION     DAORLF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26782_length_2192_cov_2.026205"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK198_26740"
     CDS             <1..970
                     /locus_tag="PK198_26740"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF42427.1"
                     /translation="FDPKTGEVLNESKLCEQGRNFCNKMWNALRLIKGWPVSEQAPDA
                     QTDAIQTIAGRWMEQRLHATIDRVNVMFKEYRLSEALMTLYKLIWDDFCSWYLEMIKP
                     AFGEPIDRATVEKASAMYEQMMALLHPFMPFVTEEIWHQLRERLEGEDCIVSSLPQGA
                     AYDDDFLQLIEEAKELVTAIREVRNNYGIKMKDPLKVFVQDSRTATGLFQTAGLQEMI
                     SKMAVLETFEITQSDIENAVAFLAGKDKFFAVVEKGLDKDEECARLRKEQEHYRGFIA
                     SVQAKLSNERFVGSAPEAVVAKERQKLADGEAKLRVIEESLAQLGC"
     gene            970..2124
                     /gene="hemW"
                     /locus_tag="PK198_26745"
     CDS             970..2124
                     /gene="hemW"
                     /locus_tag="PK198_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRF42428.1"
                     /translation="MPGLYLHIPFCRQACHYCNFHFSTSLKLKKPLLEAMEKELQGRQ
                     DFFGGPVVLDSIYFGGGTPSLLSAGELERLFERINAAFAVADDAEITLEANPDDLTDE
                     YLRALRNTPVNRLSIGIQSFADEDLQWMNRAHHAGESRRCIESALALGFDQLTCDLIY
                     GAPITSDEQWAANIQTLLDYGIPHLSCYCLTVEPKTALSHFVKTGKSPAVEEEKATRQ
                     FLYLMDALETAGYEQYEISNFAKNGLYAKHNTSYWLGEPYLGLGPGAHSYNGQNRQWN
                     LPNNPVYVKHLETPAPWESLRGLVWDEEILGAADRYNEYVMTGLRTQWGCRLEQIERM
                     GAHFAGYFRQQVQAFLHQGLVRADNDVFTLSRSGKLLADFVSAELFWVEG"
BASE COUNT          530 a          604 c          608 g          450 t
ORIGIN      
        1 cttcgacccc aaaaccggcg aagtgctcaa cgaaagcaaa ctctgcgaac agggccgcaa
       61 tttctgcaac aaaatgtgga acgccctgcg tctcatcaaa ggctggccgg tatcggagca
      121 ggcgcccgat gcacaaaccg atgccattca aacgattgca ggcagatgga tggagcagcg
      181 cctgcacgcc acgatagacc gggtgaatgt gatgttcaaa gaatatcgcc tttcggaagc
      241 cctgatgacg ctgtacaaac tcatctggga cgacttctgc tcttggtatc tggaaatgat
      301 caaaccggct ttcggcgaac cgattgaccg cgccaccgtg gaaaaggcct ccgccatgta
      361 cgagcagatg atggccctgc tgcatccttt tatgcccttt gtgaccgagg aaatctggca
      421 ccaattgcgc gaacgcctcg aaggcgaaga ttgtatcgtg agcagtttgc cgcaaggcgc
      481 tgcttatgac gacgacttcc tccaactcat cgaggaggcc aaagaactcg tgaccgccat
      541 ccgcgaagtg cgcaacaact acggtatcaa aatgaaggac ccgctcaagg tgtttgtgca
      601 agacagccgc acggcaacgg gtctgttcca aactgccggc cttcaggaga tgatctccaa
      661 aatggccgtg ctggaaacct tcgagatcac gcaaagcgac atagaaaacg cagtagcctt
      721 cttagccggc aaggacaagt tcttcgccgt ggtggaaaag ggcttggaca aagacgaaga
      781 atgcgcccgc ctgcgcaaag agcaggagca ctatcgcggg ttcattgctt cggtgcaggc
      841 caagctgagc aatgagcgct ttgtaggcag cgcgccggaa gccgtagtgg ccaaagagcg
      901 ccagaaatta gccgacggcg aggcaaaatt gcgcgtcatt gaggagtcgc tcgcgcaatt
      961 aggctgctga tgccgggttt atacctccat attccatttt gccggcaggc atgccattac
     1021 tgcaatttcc atttttctac ctcgctgaaa ctcaaaaagc ctttgttgga ggcgatggaa
     1081 aaagaattgc aagggcggca ggattttttt ggaggccccg ttgtgttgga cagcatttac
     1141 ttcggcggag gcacgccttc gttgttgagt gctggagaat tagagcgcct ttttgaacgc
     1201 atcaacgctg cctttgccgt agccgacgac gcagaaatca cgctggaggc caatcccgac
     1261 gacctgacgg acgagtacct gcgcgccctc agaaacaccc cggtcaatcg cctgagcatt
     1321 ggtattcagt catttgcaga tgaagatttg caatggatga atcgggcgca ccatgccgga
     1381 gaatctcggc gctgtataga atcggccctc gctctgggct tcgaccaact cacctgcgac
     1441 ctgatctacg gggcgcccat cacctccgat gagcaatggg cggccaatat tcaaaccctc
     1501 ttggattatg gcattccgca cctatcctgc tactgcctca cggtggagcc taagacggcc
     1561 ctgtcgcatt ttgtcaaaac ggggaaaagc cctgccgtag aagaagaaaa agctacccgg
     1621 cagtttctgt acctcatgga cgccctcgaa acggccggtt acgagcagta cgagatttcc
     1681 aattttgcca aaaacggcct gtacgccaag cacaacacct cctactggtt aggcgaacca
     1741 tacctcggcc tcggtccggg cgcacactcg tacaacgggc aaaacaggca gtggaatttg
     1801 cctaataatc ctgtgtatgt caaacacctg gaaaccccgg cgccctggga atcgctgcgc
     1861 gggcttgtct gggacgaaga aatactcggg gcggcagatc gctacaacga gtatgtgatg
     1921 acggggctgc gcactcagtg gggctgccgc ctggaacaga tcgaaaggat gggcgcacac
     1981 tttgccggat attttcgcca acaggtacaa gcctttctgc atcaagggct tgtgagggcc
     2041 gacaatgatg tttttaccct gagccgcagc gggaagttgc tggcggattt tgtgagcgcg
     2101 gagttgtttt gggtggaggg gtaggcggac cgcttggact tatgccccat tcctcacaca
     2161 agccactgct ccccaagtat ccgcgccacc tc
//
LOCUS       DAORLF010000609         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_26792_length_2191_cov_1.676030, whole genome shotgun sequence.
ACCESSION   DAORLF010000609 DAORLF010000000
VERSION     DAORLF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26792_length_2191_cov_1.676030"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK198_26750"
     CDS             complement(<1..301)
                     /locus_tag="PK198_26750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRF42429.1"
                     /translation="MRQRANSLYGISFAERFLKGYNGLTVWASANPASFFAQEKRPHW
                     LPSWLNVAVGYGAENMFGGRENRWEENGVEFGLDDSVLPRYSQYYLSLDVDFTRIK"
     assembly_gap    309..341
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<342..>712)
                     /locus_tag="PK198_26755"
     CDS             complement(<342..>712)
                     /locus_tag="PK198_26755"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRF42430.1"
                     /translation="FETWYKGFELTSFHSFDDWNEWRKMDKAGHLFTTNIESRLIFQG
                     ARWTGMNRRSAMWTGAGAGMLLQTTIEVMDGFSSEWGFSWSDMGFNVLGAGMFVAQEM
                     AWQDQRIIMKASYTEPQYADF"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(938..1528)
                     /locus_tag="PK198_26760"
     CDS             complement(938..1528)
                     /locus_tag="PK198_26760"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF42431.1"
                     /translation="MEAVEAYISKAGKWENLMRQIHSVLLETELKEEVKWGIPSYTFN
                     GSMVIGMAGFKNWLALWFYQGVFLSDPNALLVSGTEGKTKGQRQWRIKSEDEFDLAIL
                     QQFVNEAIENARAGKSITIEKNKQVEIPSELAQALKENAALAAIFEAMSPSHRREYAE
                     YVAEAKQEATRLRRVEKIVPMILSKAGLNDKYKGKS"
     gene            complement(1603..>2191)
                     /locus_tag="PK198_26765"
     CDS             complement(1603..>2191)
                     /locus_tag="PK198_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRF42432.1"
                     /translation="AHDAMVELSGSFKRSAVSLMKIANDVRMLSSGPRCGIGEIIIPD
                     NEPGSSIMPGKVNPTQPEALTMVCAQVIGNDTAVAIGGATGHFELNVFKPVIASNVLQ
                     SARLLGDACKSFNFNCAIGIEPNYSAISKHLENSLMLVTALNTHIGYEKSASIAKKAH
                     KENLTLKEAAVELGYLTAQQFDEWVRPEDMIGALK"
BASE COUNT          505 a          569 c          523 g          551 t
ORIGIN      
        1 ttttaatgcg cgtgaaatca acatccaaag acaaataata ctggctgtat cgggggagaa
       61 cagagtcgtc taacccgaac tcgacaccgt tttcctccca cctgttttcc ctgccgccaa
      121 acatattttc cgcgccgtac ccaacggcaa cattgagcca ggagggcagc caatgcggac
      181 gtttttcctg agcaaaaaac gaagccggat tggcggaagc ccaaacagtc agcccgttat
      241 agcctttcaa aaaacgctcg gcaaaggaga tgccgtacaa tgagttggcg cgttgccgca
      301 aatttgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaaatcag cgtattgcgg
      361 ctcagtataa gaggctttca taatgatgcg ctgatcctgc caggccattt cctgagctac
      421 aaacatgccg gcgcccaaca cattaaatcc catatccgac caggaaaagc cccattctga
      481 agaaaagccg tccatcactt caatggtggt ctgcaatagc attccggccc cggcgccggt
      541 ccacatggcc gatcggcggt tcatcccggt ccagcgtgcg ccctgaaaaa taagccggct
      601 ctcgatgttt gtggtgaaca gatggccggc tttgtccatt ttgcgccatt cgttccagtc
      661 atcgaaactg tgaaaagaag taagctcaaa tcctttgtac caggtctcga acnnnnnnnn
      721 nnaatggaaa cacctccata caaagctgtg cctccgctta cagaaatcca gaatcgggtt
      781 ttgtctaaag tgtcggcagg ttccaaaaag tgtcttttcg gggtctgttg cgcagtactc
      841 caaaagggca aacaacacaa ggcgatgagt gtcaatagcc ttgccatcaa gcatttagct
      901 gccggggcaa agcaggctga gatatatttc cgccccgtca agactttcct ttgtatttgt
      961 cgttcaagcc ggctttgctc aaaatcatag gcacaatctt ctccactctg cgcaatcggg
     1021 tagcctcctg cttggcctcg gccacatact cggcgtactc ccggcgatgg gatggactca
     1081 tggcctcaaa aatggcggcg agtgctgcat tttccttcag agcctgggca agttcggagg
     1141 ggatttctac ctgtttgttt ttttcgatgg tgatggattt tcctgcgcgg gcattttcaa
     1201 tggcctcatt tacaaattgt tgcaatattg ctaagtcaaa ttcatcctct gattttattc
     1261 tccattgccg ctgccccttg gttttgcctt ccgttccgct caccaacagg gcattgggat
     1321 cagaaaggaa aacgccctga taaaaccaga gtgccagcca gtttttgaaa cccgccatgc
     1381 cgataaccat agaaccgttg aaggtatagg acggaatgcc ccatttgact tcctctttca
     1441 gttctgtttc caacaaaacc gaatgaatct gccgcatcag gttttcccat tttccggctt
     1501 tgctgatgta tgcttctact gcttccatgt tttcagtttc agtgaaaagt gaaggggtgc
     1561 tgtgtgatta gcccccttca ctgtgctccc ggaacgcaaa gattatttca aagctccgat
     1621 catatcttcc ggacgtaccc actcgtcgaa ctgctgtgct gtgagatagc ccagttcaac
     1681 ggcggcttct ttgagcgtca ggttttcctt gtgcgctttt ttggcaatgg aggccgattt
     1741 ttcataacca atgtgcgtgt tcagagcggt caccagcatc agggagtttt ccaaatgttt
     1801 gctgatggcg ctgtaattgg gttcgatgcc gatggcgcaa ttgaaattga atgacttgca
     1861 ggcatcgccc aggagacgcg cgctttgcaa tacgttggag gcaatcaccg gcttgaacac
     1921 gttcaactca aagtggcccg ttgcaccgcc tatggcaaca gccgtatcgt ttccgatcac
     1981 ctgagcgcat accatcgtga gagcttcagg ctgcgtagga ttcactttgc ccggcatgat
     2041 ggacgagccg ggttcattgt cgggaatgat gatttcgccg atgccgcagc gggggcctga
     2101 actcaacatg cgcacgtcgt tggctatttt catcagtgaa acagcgctgc gtttgaagga
     2161 gcccgaaagc tccaccatgg catcgtgagc c
//
LOCUS       DAORLF010000610         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_26972_length_2179_cov_1.599812, whole genome shotgun sequence.
ACCESSION   DAORLF010000610 DAORLF010000000
VERSION     DAORLF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26972_length_2179_cov_1.599812"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PK198_26770"
     CDS             complement(<1..560)
                     /locus_tag="PK198_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42433.1"
                     /translation="MAELTVAEKLRQLYELQQIDSQVDEIQILKGELPIEVSDLEDEI
                     AGLQTRVNKLDHAVKEIQSEMSRHQGNIEHANMLIERYQTQLDNVKNNREFEALTKEL
                     ELQRLEIQLSEKKIRQSHAELEAKEETLAAARERMNKRSTELDAKRVELQQIIEKTEK
                     EEEKLRKKSDKARKGIEDRLMKAYDR"
     gene            complement(587..>1487)
                     /locus_tag="PK198_26775"
     CDS             complement(587..>1487)
                     /locus_tag="PK198_26775"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRF42434.1"
                     /translation="ITGNPDWPVRGVLCCLDSTEAIVEEAISKGCNLIVAHHPIIFSG
                     LKRLTGRTYVERTVMAAIKNDVAIYAIHTNLDNVYHQGVNAGIASVIGLKDTQILAPK
                     SILRRATATGMGAGRDESLRESGATEIVHLSDNVCEITFPIAARSRIEAVVGQAGGLL
                     SGVHAVEDAHLQTGSGMIGNLNKPMPERDFLLHLKQVMRAGAVRHTALRGREVQRVAV
                     CGGSGGFLLGQAIAQGADVFVTADYKYHEFFDADGRIVIADIGHFESEQFTIDLLHGI
                     ISEKFSTFAAYCAEARTNPVFYL"
     assembly_gap    1488..1589
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1661..>1851)
                     /locus_tag="PK198_26780"
     CDS             complement(1661..>1851)
                     /locus_tag="PK198_26780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42435.1"
                     /translation="EECLLQLSLRLPYQQVLNSAPDEVRFFMLESFLAAMESSDSELE
                     IEGFDKEGFVSDVKMVLS"
     assembly_gap    1852..1921
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          453 a          563 c          482 g          509 t
ORIGIN      
        1 atgcggtcgt aggctttcat caggcgatct tcaatgcctt tgcgggcctt atccgacttt
       61 ttgcgaagct tttcttcttc cttttccgtc ttttcgatga tctgctgaag ctctacgcgc
      121 ttggcgtcca attcggtaga gcgcttgttc atgcgttcgc gggccgccgc gagggtttcc
      181 tctttggctt cgagttcggc atgcgactgg cggatttttt tctcagacaa ctggatttcg
      241 aggcgctgca attccaattc tttggtgagc gcttcaaatt cgcggttgtt cttcacattg
      301 tccaactgag tttggtaacg ctcaatgagc atgttggcat gttcgatgtt gccttgatgc
      361 cgcgacatct ccgactggat ttccttgacg gcatgatcca acttgttgac gcgggtttgc
      421 aagcctgcaa tttcgtcctc cagatcgctc acctcgatgg gcagctcgcc tttaagtatc
      481 tggatttcat ctacttgaga gtcaatctgc tgcagttcgt acagttggcg aagtttttcg
      541 gctacggtca attctgccat tctataaagt tttaacaatg aactttttaa agataaaaaa
      601 cgggattggt gcgcgcttcc gcgcaatagg ccgcaaaggt actgaatttt tctgaaataa
      661 tcccgtgcag caaatctatt gtaaattgtt cactctcaaa gtgtccgatg tcggcaatca
      721 caatccggcc gtcggcgtcg aaaaattcgt ggtatttgta atccgccgtt acaaacacat
      781 cggcaccctg cgcaatggcc tgccccaaaa ggaagccgcc ggagccgccg cagaccgcca
      841 cacgctgcac ctcgcgccca cgcaatgccg tatgccgcac cgcaccggcc cgcataacct
      901 gcttgagatg cagcaaaaaa tcgcgctccg gcatgggctt gttcaagttt ccgatcatcc
      961 ccgaaccggt ttgcagatgg gcatcctcca ccgcatgaac gcccgacaac aggccgcctg
     1021 cctggccaac aactgcctct atccggctgc gggcagcgat aggaaacgtt atttcgcaca
     1081 cattatcaga cagatgcact atttctgttg ccccgctttc gcgcaacgac tcatcccgac
     1141 ctgcacccat gcccgtcgct gtggcgcgcc gcaagatgct cttcggggcc agtatctgtg
     1201 tatctttcag cccgataacc gaggcgattc cggcattcac cccctgatgg taaacattgt
     1261 ccaaattggt atgaatggca taaatggcca catcgttttt gatggccgcc atgacggtac
     1321 gctccacata agtgcgcccg gtgagccgtt tgaggccgct gaaaataatg gggtggtggg
     1381 ccacaatgag gttgcaacct ttactaatgg cctcttccac aatggcttcc gtgctgtcta
     1441 aacaacaaag cacgccgcgc acaggccaat ccggattgcc ggttatcnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaaatggaac cggaatgaaa acgcagagaa
     1621 gaaaaagtgg ttggaacagc gcccgaaaaa gccctgtttt ttaggaaagc accatcttca
     1681 catctgatac aaatccttct ttatcaaacc cttcgatctc caattcagag tcggaagact
     1741 ccatcgctgc caaaaaggat tccagcataa aaaagcgtac ctcgtcggga gcgctgttca
     1801 gcacctgctg gtatggcagg cgaaggctga gctgaagcag acactcctcc tnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 naaaaatgta ctgcacgccc ggcccgtatt ggatgagcgt aaaatgctga ttgagccagt
     1981 cttcgagcgc tgcgatgggc agtttgtcgg ctactttttc atcaaatacg ccactgattt
     2041 caaatgcggt atcgtccatt gtattggtgt tttgtaatca aatattcgct caaccccaca
     2101 gtgcggctat cgccaactgc ccactcccct ccccgccaag tcagggcgta acccgaagtt
     2161 acggcctgac ctcgtgtcg
//
LOCUS       DAORLF010000611         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27076_length_2173_cov_1.446176, whole genome shotgun sequence.
ACCESSION   DAORLF010000611 DAORLF010000000
VERSION     DAORLF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27076_length_2173_cov_1.446176"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK198_26785"
     CDS             complement(<1..300)
                     /locus_tag="PK198_26785"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRF42436.1"
                     /translation="MPIRRLPAEWEPQSFVQLTFPHADTDWADMLEPALACFTEIAVA
                     VSRFEEVLIVCRNVEETAARFSPEQAARIRFAELPSNDTWARDHGGITIFENEKPV"
     assembly_gap    339..531
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<532..>787)
                     /locus_tag="PK198_26790"
     CDS             complement(<532..>787)
                     /locus_tag="PK198_26790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42437.1"
                     /translation="LAAEKLMASWTAEKDRAVLQQMHTEDFVECDFTAVAPKDNLGDL
                     VAVIAKSKRNLFPVLDAEERFLGVVLLDDIRSTMFRTDLYS"
     assembly_gap    788..894
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<895..2101)
                     /locus_tag="PK198_26795"
     CDS             complement(<895..2101)
                     /locus_tag="PK198_26795"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRF42438.1"
                     /translation="MTWEIILHKIADWRQNRVSDRNFLIVASLIIGTVVGIVALLLKM
                     TVHKIEHTLQEIQADGKGFLFFIFPVIGILLTVLYVQRFRGGKLGRGVDQILYAVLRK
                     GGVVERDKMYSHWISSAITVGFGGSAGLEAPIVVTGSAIGSNIATELGLTKQQRTVLL
                     AAGAAAGISAVFNCPIAGVIFAVEVLLVEFSIPVFIPLLIAAATASVWSELLYKDRLF
                     YLVAQEWQVKAIPFYALLGVVTGLVSVYKARVIYYAENFFAKQKHPWRKAIVAGTALG
                     LLIFLLPPLYGEGYVTIKNLFAGKSSFLLHNGPFHALRFQEWAILLFAVLIVLLKVFA
                     TMLTTGAGGNGGIFAPSLFTGGVTGFAFSYLINLTGLVQLTTANFVAAGMAGVLSGVI
                     HAPLTGIFLI"
BASE COUNT          476 a          526 c          491 g          380 t
ORIGIN      
        1 gacgggcttt tcgttttcaa aaatcgtgat gccgccatga tcgcgcgccc aggtgtcatt
       61 actgggcaac tcagcaaaac gaatacgcgc cgcctgttca ggactgaaac gagcggctgt
      121 ttcctccaca ttccggcaca cgatgagcac ctcttcaaaa cggctgacgg caacagcaat
      181 ctcggtgaaa caagccagcg caggctccag catatcggcc cagtcggtat cggcatgagg
      241 aaaagtgagt tgtacaaaac tttgcggttc ccattcggcg ggcaatcggc ggatcggcat
      301 tatgttatac tccggtttgg gcagactggc tgagttggnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgaatataa
      541 gtcggttctg aacatcgtac tgcggatgtc gtctaacaat accacgccca gaaagcgctc
      601 ttctgcatcc agcacgggaa acaaattgcg cttggatttt gcaatcaccg ctacgaggtc
      661 gcccagattg tctttgggag ctactgcggt gaaatcgcat tctacaaagt cctccgtgtg
      721 catttgttgc aatacggccc ggtctttttc ggctgtccaa ctggccatca gcttctccgc
      781 ggccagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgatga
      901 ggaagatgcc cgtgagcggc gcgtgaatca ctcccgacaa cacgccggcc atgcctgccg
      961 ccacgaaatt ggccgttgtc agttgcacca aaccggtgag attgatcagg tacgaaaacg
     1021 caaagcccgt gacgccgccc gtgaacagcg agggtgcaaa aatgccgccg ttgccgcccg
     1081 cgccggtagt gagcatggtg gcaaacacct tgagcaacac gatgagaacg gcaaaaagca
     1141 aaatggccca ttcctgaaat ctgagcgcgt gaaaaggccc gttgtgcaac aaaaaagaag
     1201 actttccggc aaacaggttt ttgatcgtca catagccttc tccgtacagc ggcggcaaca
     1261 aaaaaatgag caggcccaag gcagtgcccg ccacaatggc ctttcgccag ggatgttttt
     1321 gcttagcaaa aaaattctcg gcgtaataaa tcactctggc tttataaacc gacactaagc
     1381 ccgtgaccac ccccaacagc gcgtaaaacg ggatggcttt cacctgccac tcctgcgcca
     1441 caaggtagaa caagcggtct ttgtacagca attccgacca tacagaggcc gtggctgccg
     1501 ctatgagcag aggtatgaac accgggatgg aaaactccac gagcaatacc tccaccgcaa
     1561 aaatgacccc cgcaatcggg cagttgaaca ccgccgagat gcccgccgca gcgccggccg
     1621 ccagcaatac cgtgcgttgc tgcttggtga gacccaactc tgtggcgatg ttggaaccta
     1681 tggccgagcc tgtgacgacg atgggcgcct ccaatcctgc cgaaccgccg aagcccaccg
     1741 taatggcgct gctgatccag tgcgaataca tcttgtcgcg ctccaccacg ccgcctttgc
     1801 gcagcacggc atacaaaatc tgatctacgc cgcgccccag cttgccgccc cgaaagcgct
     1861 gcacatacag caccgtgagc aaaatgccga tgacgggaaa aatgaaaaag agaaagcctt
     1921 tgccgtcggc ctgtatttcc tggagggtgt gttctatttt gtgtaccgtc attttgagaa
     1981 gcagggccac aatgcccacc accgtgccga tgatgaggct ggctactatg agaaaattgc
     2041 ggtcgctgac gcggttctga cgccagtcgg ctattttatg aagaatgatt tcccaagtca
     2101 tgggcgcaaa ggtaagcatt agtagtgggg caaaaataaa tgctcatttt ctttaggcca
     2161 aaggattaga taa
//
LOCUS       DAORLF010000612         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27133_length_2170_cov_2.002837, whole genome shotgun sequence.
ACCESSION   DAORLF010000612 DAORLF010000000
VERSION     DAORLF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27133_length_2170_cov_2.002837"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1739)
                     /locus_tag="PK198_26800"
     CDS             complement(174..1739)
                     /locus_tag="PK198_26800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF42439.1"
                     /translation="MPANNRSEHTLFILSALLLPAGLLLNLGTQPLYLEEPRRALIAM
                     EMWERSNWWVPTELGDFYYNKPPFFNWILMLSAALLGGFHEWAMRLPTVLSTLGMAAL
                     LYFMGQRYVDAAFGRISALLFLSCGSVLLFFSHLGEIDLFYAFISFGGFAALFHFYQI
                     RKDGLLFLVFYALHAVGFLTKGLPSLLFIALSLSAWFAWQRDFRRFFSWAHLAGIGLF
                     AALIGGYLYQYSRFHSLALLLPQWVGQAGERTVAEQGWQKLAFHLVHYPLDTLKDLLP
                     FSLLILFLLRRDWLPLLQRNAWVGFSALIFAANFPVYWLSPGAKQRYLYMLFPLIIVA
                     LVWAWRHAQELKPWRSKAFRVLVGVFVGALALGSPAIGFADDLAFLSYRHWLVFGSAA
                     VFGMLFWTWLRRKLPDLYALVLAMAILRIVFDLSVLPQRAQHSGAQYDRQTAVDIARI
                     TAGKPLYIYGDERISFTTVYYLNRLRGQVLVRNAEAKKGVFYIAPDDHSAPLRTVYYR
                     FEYDKAPYVLFQL"
     gene            complement(1767..>2170)
                     /locus_tag="PK198_26805"
     CDS             complement(1767..>2170)
                     /locus_tag="PK198_26805"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRF42440.1"
                     /translation="AKVSAWKESALPDSIVSLPQLESYLNQITRKMRRPFAFQVKGQV
                     ETAQIHIVNLPPGTKVSSPDEAHQGIQHYTVSNTEVLMLGFFSTEHQAVFTHHDTFMH
                     LHLMTEDKKQMGHLEEARFKPGQMRLLLPGK"
BASE COUNT          566 a          597 c          601 g          406 t
ORIGIN      
        1 tggtttccat ctgtttttta ccgggatggg ctgcgactgc ctggtcggcg gagggctgat
       61 gttggcagcc gtaaaaggat aatgcgatga tgagcaggag aaaaaagtac ttcatgcgag
      121 cggaatttga ggcaaagata agcctgctgc tccggcgtga tttgtaatgc ccgtcacaat
      181 tggaagagaa cataaggcgc tttgtcgtat tcaaacctgt aatacaccgt cctcaatggg
      241 gctgaatggt cgtctggagc gatgtaaaaa acgccctttt tcgcttccgc atttcttacc
      301 aaaacctgcc cgcgcagccg gttgaggtaa tacacggtgg tgaaagaaat tcgctcatcg
      361 ccatagatgt acaaaggctt tccggcggtg atgcgggcta tgtcaacggc cgtttgccgg
      421 tcgtattgcg ccccgctgtg ctgtgctctt tgtggcaaca cactgagatc gaacacgatg
      481 cgcaaaatgg ccattgccag cacaagcgcg tacagatcgg gcagtttccg ccgcagccaa
      541 gtccaaaaca acatgccgaa caccgccgcc gacccgaaaa cgagccagtg ccggtaggac
      601 aaaaaggcca gatcatctgc aaaaccaatg gccggactgc cgagcgccaa tgccccgaca
      661 aaaacgccga ccaaaaccct gaatgccttg ctgcgccagg gtttcagctc ctgagcatgc
      721 cgccaggccc agacgagcgc cacgatgatg agcggaaaca acatatagag gtagcgctgt
      781 ttggcgccgg gcgaaagcca atacaccggg aaattggccg caaaaatgag cgccgaaaac
      841 cccacccagg cattgcgttg aagcaaaggc agccagtcgc ggcgcagcag aaacaggatc
      901 agcagcgaaa agggcagcag gtctttgagg gtatccaaag gataatgtac gaggtgaaag
      961 gccaattttt gccaaccttg ctcggccacc gtgcgctcac ccgcctgccc gacccattgc
     1021 ggcaacagca aagcgaggct gtggaaccgg gagtattggt acaaataccc gccgatgagc
     1081 gccgcaaaca gcccgatgcc cgccagatgc gcccatgaaa aaaatcgcct gaaatcgcgc
     1141 tgccaggcaa accaggcgct cagcgacaag gcaatgaaca gcagcgaagg cagccctttg
     1201 gtgagaaacc cgacggcatg gagggcataa aatacgagga acagcagccc gtctttccga
     1261 atctggtaga aatgaaacag cgccgcaaaa ccgccgaaac tgatgaaggc ataaaacaag
     1321 tctatctcgc ccagatgcga aaaaaagagg agcacgctgc cgcaggacag gaacaacagg
     1381 gcgctgatcc tgccgaatgc agcgtccaca tagcgctgcc ccatgaagta cagcaaggcg
     1441 gccatgccca gcgtagagag cactgtaggc aggcgcatgg cccactcgtg gaagccgccg
     1501 aggagggccg cgctcagcat cagtatccag ttgaaaaacg ggggtttgtt gtagtaaaaa
     1561 tcgcccaatt cggtaggtac ccaccagttg ctgcgctccc acatttccat cgcgatgagg
     1621 gcgcggcgcg gctcttccag atacaagggc tgtgtgccca aattcagcag caagcctgcc
     1681 ggcaagagca gcgctgaaag gatgaaaagg gtatgttctg agcggttatt ggcgggcatt
     1741 gcagcgtatt ttcaggcgac tgatattcac tttccaggca acaacagacg catctgcccc
     1801 ggcttgaacc tcgcttcctc caaatgcccc atttgcttct tgtcttccgt catcaggtgc
     1861 aggtgcataa aagtatcgtg gtgcgtaaat acagcctgat gttcggtaga aaaaaagccg
     1921 agcatcaaaa cctctgtatt cgacacggta taatgctgaa tgccctgatg cgcttcgtcg
     1981 ggcgagctga ccttcgtacc cggcggcaaa ttgacgatgt gaatctgcgc tgtttctacc
     2041 tgccctttca cctgaaacgc aaaaggtcgc cgcatcttcc gggtgatctg attcagatag
     2101 gattccaact gcggcaggct gacgatgcta tcgggcagcg cagattcctt ccatgcggac
     2161 accttggctc
//
LOCUS       DAORLF010000613         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27486_length_2148_cov_2.846632, whole genome shotgun sequence.
ACCESSION   DAORLF010000613 DAORLF010000000
VERSION     DAORLF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27486_length_2148_cov_2.846632"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(583..1395)
                     /locus_tag="PK198_26810"
     CDS             complement(583..1395)
                     /locus_tag="PK198_26810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42441.1"
                     /translation="MEIQVLISKKGTKVVIATQLHQALELPHQHYPVNVRKWIKDAYS
                     FQDGIRRPIAMKDYGVRKWKDNPIVEDYYLTVELAKHIALHSRSRVKMDIARQLAAHD
                     DTQEREGMLSKEQVLAVLDLTKAMGLVSCQDACERRHLETYEERNGGRSTDWWQYRSE
                     VMGYSVEELRQRLSLSGQKVAGKNRRQLLMQLDKHEIVRSAVIDLFMSMGKSESYART
                     LGDLAKVFAQEMNVEIYDDRTNEPVLPLGPSINPAVVNEVKAGKREAVLACW"
     gene            complement(1760..>2148)
                     /locus_tag="PK198_26815"
     CDS             complement(1760..>2148)
                     /locus_tag="PK198_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF42442.1"
                     /translation="ARIEAGEGLLVVCYGMGVHWALHAAANFAGRISVLDLRSLYPLD
                     TDMIFEQSQRHGRVLVITEEPVNNTFAQSIAARIQEHCFEYLDAPVRTIGAENLPAIP
                     LNETLEKTMLLSSHKVADAIERLLTY"
BASE COUNT          547 a          509 c          523 g          569 t
ORIGIN      
        1 ccagctgatt acaaatcagc tgctctacca actgagctac actggcttta tggcagttag
       61 tatctggagt agcggatgta aaaattcgct attctaaaaa cgatgtgcca aagcagatgt
      121 atttgaacta cttttcaaag tcattgattt tcagtactta aactattgaa aaccaactta
      181 atttgccttt gatcggaact tttcccggaa agcgattgca aagatacgac tattgccctc
      241 aattttccaa gtgaaaaaat atttttttat agatttttat tctgcgcatc acggattgcc
      301 gcccggcaga ggaaacaaaa gtctgtgcgt aaaaatttca ctctgcttta gacctgatta
      361 caaccaatat ctgacggcaa atgtgacctt gaggcttatt ttttacaata aaacaagagc
      421 ctgctatccg ttgccggaag caagctcttg aatggattat aatatcaggc aaagaggggt
      481 tataccttgc gcagccaagt tttgcgcata gccttcggca cccgcgccat ggcgcggtgg
      541 ttagcagcgc ccggcttcgc acctgacggt gtgaggtaca tattaccaac aggcaagcac
      601 ggcctcccgt tttccggcct ttacttcatt tacgaccgca ggattgatgg aaggccccag
      661 cggaagcaca ggttcattgg tgcgatcatc ataaatttcg acattcattt cctgagcaaa
      721 aactttggcc aagtctccca gtgtacgggc atacgattct gatttaccca tagacataaa
      781 aaggtctatc accgccgagc gcacgatttc gtgtttgtcc aattgcatca acagttggcg
      841 gcgattcttt ccggctactt tttgcccgct gaggctgagc cgttggcgca attcctccac
      901 agagtacccc atcacttccg agcgatattg ccaccagtcg gtggaccggc cgccgttgcg
      961 ctcttcatac gtttccagat gccgacgttc gcaggcatcc tgacacgaaa ccaagcccat
     1021 tgctttggtc agatcgagta cggccagtac ctgctctttg ctcaacatac cttcgcgctc
     1081 ctgtgtatcg tcatgtgcgg ctaattggcg ggcgatgtcc attttcaccc ggctgcggga
     1141 atgcagggca atatgtttgg ctaattctac ggtgaggtaa taatcctcca cgattggatt
     1201 gtctttccat ttgcgcacgc cataatcttt catggcgatg gggcgacgga tgccgtcctg
     1261 aaagctgtaa gcatctttga tccacttgcg cacattgacc ggatagtgct gatgcggcag
     1321 ctccaatgcc tggtgaagct gcgtggcgat gactactttg gtgccttttt tgctgatgag
     1381 aacttgaatt tccattgtgt cggaagtttt attcttgcga ttacaatgca aatatacacg
     1441 cttttgtttt taaaagcaat tttttaaaac atttttttta ataaaaatac aatttcataa
     1501 aacggggtca ggcagcatgg cggcgataca agtccaaaaa aaagtataaa atattgaaaa
     1561 taaataaatt atgagcacat agaatcaact tgtgcgtaaa catttgagac gactacctga
     1621 atatggatac agctcttcaa tagcaggaaa ccctttgcgt gagggggccg agggtaacgc
     1681 agccgatagg cgaagtgacg gaggcacccg acggctacct gagtttttgt aagagtagcc
     1741 ggcacgccca aaaacaaatt taatatgtca gcaaacgctc tatggcatcg gctactttgt
     1801 gggaggagag cagcatggtt ttttccagcg tttcgttgag cggtatggcg ggcaggtttt
     1861 cggcgccaat ggtgcgcacc ggagcgtcga ggtattcgaa gcaatgctcc tggatgcggg
     1921 cggcgatgct ttgggcgaag gtattgttta ctggctcttc tgtgatgacc aggacgcggc
     1981 catgccgttg ggactgctca aaaatcatgt ctgtgtctaa tgggtacagg ctccgcagat
     2041 cgaggacgga aatgcgcccg gcgaaattgg cagcagcgtg gagcgcccaa tgcacgccca
     2101 tgccgtaaca aacaacgagc aagccttcgc cggcttctat acgcgcag
//
LOCUS       DAORLF010000614         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27640_length_2138_cov_1.726836, whole genome shotgun sequence.
ACCESSION   DAORLF010000614 DAORLF010000000
VERSION     DAORLF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27640_length_2138_cov_1.726836"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK198_26820"
     CDS             <1..514
                     /locus_tag="PK198_26820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF42443.1"
                     /translation="AIFNGLGRIAWGMISDRIGRRPAIIIMSAFQGVVMLALYHGFIT
                     FGLATGLIVGASLVGFNYGGIFALFPAITADYFGNKQVGANYGWVFTAYGLAGLMGPL
                     LAGVFKDNAADGGSPVVWMAPFIIAGAACLIGAVVMVFTHPPKKPAALVQPASLADVA
                     QRQEPSLEKV"
     gene            660..>947
                     /locus_tag="PK198_26825"
     CDS             660..>947
                     /locus_tag="PK198_26825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF42444.1"
                     /translation="MQPVKDLFSEGAEQYAQFRPSYPLELYEFLYAHLDHFGLAWDVG
                     TGPGQVACALSTRFEQVIATDISAPQIANAERRPNIRYIVCRAEQTPFPDAS"
     assembly_gap    948..985
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <986..1407
                     /locus_tag="PK198_26830"
     CDS             <986..1407
                     /locus_tag="PK198_26830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF42445.1"
                     /translation="DPVPFYKEARRTAKPGAVIAVWGYDLLEIGPHVDELITAYHRDV
                     LGQYWDYERRYVDDRYADFPFPFEDIPCPGFTMRMNWTLTQLEGYLNTWSALRNYRRQ
                     TGRDPLPALIAHIAEHWPAGEVHEVRFPVFVRVGRVA"
     gene            1569..>2138
                     /locus_tag="PK198_26835"
     CDS             1569..>2138
                     /locus_tag="PK198_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF42446.1"
                     /translation="MSKSIIVLVAALLVSISMPAQSLKDLANKAKNTLTGGAPLSEAE
                     IGDALKEALDKGAGDAVSFLSAPDGYYKTAYKILLPEEAQQVTAKLKAVPGFENVEAQ
                     LIEKMNRAAESAAAKAKPILVDAIRQMTIRDAMNLLMGEKDAATRYLERTTSEQLYLA
                     FKPVIVQALDEVNAREYWRSAVTAYNRIPL"
BASE COUNT          461 a          594 c          575 g          470 t
ORIGIN      
        1 tgctattttc aacgggctgg ggcgcattgc ctggggtatg atctccgacc gcatcgggcg
       61 caggcccgcc attattatta tgtcggcgtt tcagggggtg gtcatgctgg cgctgtatca
      121 cgggttcatc accttcggtt tggctacggg tctgatcgtg ggcgcttcct tggtggggtt
      181 caactatggg ggcatttttg cgctgtttcc ggccattacc gccgactatt tcggcaacaa
      241 gcaggtaggc gccaattacg gctgggtgtt cacggcttat ggtctggccg gcctgatggg
      301 gccactgttg gcgggcgttt ttaaagacaa tgccgccgac ggaggttcgc ccgtcgtatg
      361 gatggcgccg tttatcatcg cgggggctgc ctgcctcatt ggtgcggtgg tgatggtgtt
      421 cacacatccg ccaaagaaac cggctgcatt ggttcagcct gcgtccttag ctgatgttgc
      481 gcaaaggcag gaaccgagtt tggagaaagt ctgatgcgcg cgggcgttac tgaatgatct
      541 ggaattagat tttgcgattg tctacattca gacttctttg ataatgacat atttatgacc
      601 ttgcagtctg ctggaaatgc ccgtactttg tcgtttcaaa ccgacaataa aaagtcacca
      661 tgcaacccgt caaagacttg ttttcggaag gtgctgaaca atacgcgcaa ttccggccat
      721 cctacccgct cgaactgtac gaatttctct acgcacacct cgatcacttc ggcttagcct
      781 gggatgtggg tacggggccg gggcaagtgg cctgcgcatt gtccacccga tttgagcaag
      841 tcattgccac cgacatcagc gcgccgcaaa tagccaatgc cgagcgccgt cccaacatcc
      901 gctacatcgt ttgtcgcgcc gagcaaacgc ctttccccga cgcctccnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnntcgat cctgttccat tttacaaaga agctcgtcgt
     1021 acggccaaac ccggcgccgt cattgccgtt tggggctacg atctcctcga aataggacct
     1081 catgtggatg aactcatcac cgcctaccac cgcgatgtgt tgggccaata ctgggactac
     1141 gagcgccggt atgtggacga ccgctacgcc gatttcccat tcccctttga agacattcct
     1201 tgtccgggct ttaccatgcg catgaactgg acgctgacgc agttggaagg ctaccttaac
     1261 acttggtcgg cactgcgcaa ctaccgtcgc caaacgggcc gtgacccact gccggccctc
     1321 attgcccaca ttgcggagca ttggcccgcg ggggaggtgc atgaagtgcg ttttcctgtt
     1381 tttgtgcgcg tggggagggt ggcgtagggg gcagatggag caaagcgaca tcccggatag
     1441 cctgtttcga taaaatcgga aaggaacgaa tcgggagggc gttttaccgg gcgctgcttt
     1501 ttaacatcgg cttaacgagc agaaatgcga cttgggcgtt gtatgggaaa ccaaaataca
     1561 agaagaacat gtccaaatcc attattgtac tcgttgcggc actgctcgtc tccatctcca
     1621 tgccggcaca aagcctgaaa gacctcgcca acaaagccaa aaacacgctc accggcggcg
     1681 ccccgctttc ggaggcagaa ataggcgatg cgctcaaaga ggcattagac aaaggcgccg
     1741 gcgatgcggt cagcttcctc tccgcccccg acgggtatta caaaacagct tacaaaatcc
     1801 tgctgccgga agaggcgcag caggttaccg ccaagctcaa agcagtaccg ggctttgaaa
     1861 acgtagaggc ccaactcatt gaaaaaatga accgtgcagc ggaatcggct gccgccaaag
     1921 ccaaacctat tctggtggac gccatccgcc aaatgaccat ccgcgatgcc atgaacctgc
     1981 tgatgggcga aaaagatgcc gccacgcgct acctcgaacg caccacttca gagcagcttt
     2041 atctcgcctt caaacccgtc atcgtacagg ctttagacga agtgaatgcc cgcgaatact
     2101 ggcgcagcgc cgtcacggcc tacaaccgca ttccttta
//
LOCUS       DAORLF010000615         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27641_length_2138_cov_1.588094, whole genome shotgun sequence.
ACCESSION   DAORLF010000615 DAORLF010000000
VERSION     DAORLF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27641_length_2138_cov_1.588094"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PK198_26840"
     CDS             <1..1087
                     /locus_tag="PK198_26840"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRF42447.1"
                     /translation="RTPMHAGKPLSSSEIYRRSLRKKRLFVWVALALVLVLLLFGLTA
                     GALKLSFAEVWAALFGGQNDTHRHIVLNLRLPRLLAAIVAGGALGLAGAVMQIVLRNP
                     LGSPFTLGISNAAAFGAALAYLMLGSPILQSVHHWLPGVQNSVVAISAFIWAALGSFF
                     IIFISRKKGATPENMILSGIIINTFFMALTSILQFLADDVQLGSIVFWTFGDLAKSTW
                     NTLAIQVLVILPVLLFFLKNGLRFNALDAGDQVASSLGISVHKFRLYSILLTSLLTAC
                     IVSFYGIIAFVGLIIPHVVRILMGNDARFLMPASAALGALFLLICDLVSRSLFSPLVI
                     PVGIITSIIGAPLFVILLIKGSQRWSR"
     gene            1090..>1541
                     /locus_tag="PK198_26845"
     CDS             1090..>1541
                     /locus_tag="PK198_26845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF42448.1"
                     /translation="MHKLSYAYGSRQVLRDVSHVFQPGAFTAIMGQNGSGKTTLLRCI
                     NKMATDYQGLIEINGKDVRSLSLEEMAKTSAFVPQGQDAVFGASVFETVLLGRLPYMT
                     WAPAETDYRIVDEVLDRLHLLRFAATDIHQISGGERQKVYIARAVAQKT"
     assembly_gap    1542..1676
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          404 a          500 c          558 g          541 t
ORIGIN      
        1 ccgaaccccg atgcacgccg gtaagccctt gtcttcgagc gagatataca ggcgaagtct
       61 gagaaagaag cgcttgtttg tctgggtagc gctggccttg gtgctggtat tgctcctgtt
      121 cggcctgacg gcaggcgcgc tgaagttgtc gttcgctgaa gtttgggcgg ccctgtttgg
      181 cgggcagaac gatacgcatc ggcacattgt gctgaatctg cgcttgccgc gcttgctggc
      241 ggccattgtt gcgggtggcg ccttgggact ggccggcgcg gtaatgcaaa tcgtactgcg
      301 caatccactg gggtcgccct tcacgctggg tatctccaat gcggcggcat tcggcgcggc
      361 gctggcatat ttgatgctgg ggtcgccgat actccaatct gtgcatcatt ggttgccggg
      421 agtacaaaac tccgttgtag cgatttctgc tttcatctgg gcggcgctgg ggtctttttt
      481 catcattttt atttccagaa aaaaaggcgc tacccctgaa aacatgatcc tgtcgggcat
      541 catcatcaat acttttttca tggccctcac gtccattctg caatttctgg ccgacgacgt
      601 gcagttaggc tccatcgtat tctggacctt cggcgatctg gccaaaagca cctggaacac
      661 actggctata caggtattgg tcatccttcc ggtcttgctg ttttttctca aaaacggcct
      721 gcgattcaat gcgttggatg ccggcgatca ggtggcctcc agcttgggca tatcggtaca
      781 caagtttcgg ttgtacagta ttttactcac atcgctgctc acggcctgca tcgtttcttt
      841 ttacggcatc atcgcgtttg tagggcttat cattccgcat gtcgtcagga ttttgatggg
      901 caacgacgcc cgttttttaa tgcccgcatc ggcggccttg ggagctttat ttttattgat
      961 ttgcgatttg gtgtcgcgca gtttattcag tcccttagtc attccggtgg gcatcatcac
     1021 ctccatcata ggggcgcccc tgtttgtcat tttactcatt aaaggcagcc aaagatggtc
     1081 tcggtagcat tgcacaaact cagttatgct tatggcagcc ggcaggtatt gcgcgatgtc
     1141 agccatgttt ttcagccggg cgcttttacg gccatcatgg gacaaaacgg cagcggaaag
     1201 acgacgctgc tgcgttgcat caataaaatg gcgactgatt atcaggggct tattgagatt
     1261 aacgggaagg atgtgcgctc cttgtctttg gaagaaatgg ccaaaacttc cgccttcgta
     1321 ccacagggac aggatgcggt gtttggcgca tctgtttttg aaaccgtctt gctggggcgc
     1381 ctgccgtaca tgacctgggc gcccgcagaa acggactatc ggattgtgga cgaagtattg
     1441 gacaggctgc atttgctgcg ctttgctgcc accgacatcc atcagatcag cggcggcgaa
     1501 cgacagaagg tctatattgc cagggcagtg gcccaaaaaa cnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaacc
     1681 tggctttgca gtcttgcagc cattttgttt tcatcaaaaa cggccggttg atttcggtgg
     1741 ccgatccctc cggcatagat gaggcgctgg tgcaggatgt gtttgatgtg caggcgcagc
     1801 aaatgacggg cgagaacggg cgacgtttat tttcgctatg gaagaagggg taggcacagg
     1861 tggttcaatg atttgtaatt agaaagcatg actcccgatt tgccagggat aaccctcaga
     1921 ttttcaaatg cgaatcgtca caaaacggcc ctttcgcgca ttgtttgcat tggcacaggc
     1981 ggtattctcg ctgtttggat atggtaaatt tcagcggttc aaacttggtg ccgtggtgcg
     2041 aaccgtcgca gaaaggctgg tttcggctat ggccgcaggt gcaatgtaag caaccggtga
     2101 accgcatcta ttccagcacc tccctacatc cgatcttt
//
LOCUS       DAORLF010000616         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27810_length_2127_cov_2.000965, whole genome shotgun sequence.
ACCESSION   DAORLF010000616 DAORLF010000000
VERSION     DAORLF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27810_length_2127_cov_2.000965"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK198_26850"
     CDS             complement(<1..890)
                     /locus_tag="PK198_26850"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF42449.1"
                     /translation="MPENIPGYPSLADRQALGKRTAEKLSSLVDVFDMANLLTATGKY
                     AEAYEFYRYVLMQYQSREIYNNLGVAAVLDALQYFNPNELKFRFPLELDLESSASKGD
                     GMANVRERILRQAILHFDAAISLDPNYAPAYVNKACAYALLDDYTRARFYASAEAKQA
                     AQRGQYPKIARDADVLLGILEAREGRTAQADTLFRNAAQQGDYLAAYNLKVLRNEPLS
                     PERAAPAGLVRERIAGKALSDLSSEPPFDPGKKVAVGSALDFYQFTGFDQPARLFYSH
                     NVKTGDLHFYQLTAPGYQGKT"
     assembly_gap    920..947
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<948..1476)
                     /locus_tag="PK198_26855"
     CDS             complement(<948..1476)
                     /locus_tag="PK198_26855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42450.1"
                     /translation="MRYTNILLPIMLLLPLLGFAQERSFGTLPLAQKPGASALRGPDL
                     KDSPQYVRSLAIYNKLVQARGDFRYPVPNFFMRREERRVAGIDYDRLEIVLEEKAYEV
                     CVGFGEESEAAIAFLLGHELTHYYEKHVWQKGFAAEYKDLEIGSMKLDSLADAARRET
                     EADYLGGFLAYSAGFG"
     gene            complement(1610..>1799)
                     /locus_tag="PK198_26860"
     CDS             complement(1610..>1799)
                     /locus_tag="PK198_26860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42451.1"
                     /translation="MSKQGAMRLYRAKTIQEAGAVIRFCAIEGHLGATLIHEGAYAGT
                     AGFFMQDKDWNDVKVLYY"
     assembly_gap    1800..1882
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1883..>2127)
                     /locus_tag="PK198_26865"
     CDS             complement(<1883..>2127)
                     /locus_tag="PK198_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42452.1"
                     /translation="TTLQDAVRILEDLAPGDTVRLTLKQGLARKEVALVSGDLEQKRL
                     EQIAHHFFRSYTDYSLSENASGILLSSAKPKSRIQQF"
BASE COUNT          453 a          538 c          559 g          466 t
ORIGIN      
        1 gttttgccct gataccccgg cgcggtaagt tgataaaaat gcagatcgcc ggtttttacg
       61 ttgtgactgt aaaaaagacg ggccggctga tcaaaacccg taaactgata aaaatccagc
      121 gcgcttccga cagccacttt tttgcccgga tcaaaaggcg gctctgaaga caaatcagac
      181 agcgccttgc cggcaatgcg ttctcggacc aagcccgcgg gtgcagctct ctccggcgac
      241 agcggttcat tgcgcaacac tttgaggttg tatgcggcca agtagtctcc ttgttgggct
      301 gcgttgcgga acagtgtgtc ggcctgtgcg gtgcgtccct cacgggcttc caaaataccg
      361 agcagcacat cggcatctct ggcgattttg gggtattggc cgcgctgagc agcttgtttc
      421 gcttcggcag atgcgtaaaa acgggcgcgg gtgtaatcat ccagcagggc gtaggcacag
      481 gctttgttca cataagccgg cgcgtagttc gggtcgaggc tgatggctgc atcgaaatgg
      541 aggatggcct gtcgcaatat ccgctcgcgc acattggcca tgccgtcgcc tttagaggca
      601 gaggattcca gatcgagttc cagcggaaag cgaaatttca actcattggg attgaaatat
      661 tgcagggcat cgagtacggc agccacaccg aggttgttgt agatttcgcg gctttggtac
      721 tgcatcaaaa catagcgata aaactcataa gcctccgcgt atttgccggt ggcggtcagc
      781 agattggcca tatcgaacac atccactaag ctgctcaatt tttccgccgt acgtttgccc
      841 agcgcctgcc ggtcggcgag cgagggatag ccgggaatgt tttcaggcat accgtaggct
      901 ttgtacagac gggcaagcan nnnnnnnnnn nnnnnnnnnn nnnnnnnaac caaagcccgc
      961 agaataggcc aaaaatccgc ccagataatc ggcctcggtt tctctccgtg cggcatcggc
     1021 taagctgtcc aatttcatgc tgccgatttc aaggtcctta tattctgccg caaacccctt
     1081 ttgccacaca tgtttttcgt agtaatgcgt cagttcgtgg cccagcaaaa aggcgatggc
     1141 tgcctctgat tcctcaccaa aaccgacaca cacttcgtag gctttttctt ccagtacgat
     1201 ttcgagcctg tcgtagtcaa tgccggctac gcgtcgctcc tctcgacgca tgaagaaatt
     1261 aggtaccgga taccgaaaat ctccgcgagc ttgtacaagc ttgttgtaaa tggccagcga
     1321 tcggacatac tgcggcgagt ctttcaaatc gggaccccgg agggcagacg cgccgggctt
     1381 ttgagccaat ggcagggtgc cgaaactgcg ctcctgtgca aaaccgagca gcggcaaaag
     1441 gagcataatg ggtaaaagga tgttggtgta tctcatgatg aatttttttt tggtaggcag
     1501 tagccgcgcc gtggcgcggg gtgctaagcg tagagtgatc acagccagta gccagtaact
     1561 tgaggcttgt ggccagtagc ttagggcaag tagccgttcc aacccgaaat caatagtaca
     1621 aaactttgac atcgttccag tccttatcct gcataaaaaa gccggccgtg cccgcgtatg
     1681 ctccttcgtg gatgagtgtg gcgccaagat gcccctcaat ggcgcaaaac cggatgacgg
     1741 cccccgcttc ctgaatcgtt ttggcccggt agagcctcat tgcaccctgc ttgctcatgn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nncaaactgc tgaatgcggg acttcggttt ggcactgctc
     1921 aagaggatgc cggaagcatt ttccgacagg ctgtagtcgg tataactgcg aaaaaaatgg
     1981 tgcgcaattt gctctaagcg cttttgttcc aagtcgcccg aaaccagcgc tacctccttg
     2041 cgggcgaggc cctgtttcag cgtcagtcgc acagtgtcgc cgggagcgag gtcttccaga
     2101 atgcgcactg catcctgcag ggtggtc
//
LOCUS       DAORLF010000617         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_27890_length_2122_cov_1.941945, whole genome shotgun sequence.
ACCESSION   DAORLF010000617 DAORLF010000000
VERSION     DAORLF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27890_length_2122_cov_1.941945"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..995
                     /locus_tag="PK198_26870"
     CDS             168..995
                     /locus_tag="PK198_26870"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF42453.1"
                     /translation="MKKVSFSLACSLLLASSALLTAQKAELGFMGGISLYNGDLAPKE
                     YIDYLKEVNPAFGIFGRVNVNPYFSVRAGITQAKVTGDDINSFPSRGLNFRSNITELA
                     LTGEYSPFQLGGQRAKLVTAPYLYAGATVYRFNPQTLYDNNWIDLRPLGTEGQGLPGY
                     EKPYSLTQIAVPIGAGIKFTVRQTWSLGIEFGARKLFNDYLDDITSAQVNYLDVLTGN
                     GTLAATLSNPSIKEPTDATYRRGGEFQDWYYVGGVTLSFRLAETRSRSGRGLGCPEF"
     gene            1054..1407
                     /locus_tag="PK198_26875"
     CDS             1054..1407
                     /locus_tag="PK198_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF42454.1"
                     /translation="MSHTAEIASTPVTLTEGALHELARIRKEQNIPAEYGLRIGVKGG
                     GCSGFSYILGFDEPKDGDDTFQINGMKVLMQKAHAIYLLGMEIDWVEGLNNRGFSFNN
                     PNATETCGCGTSFSA"
     gene            complement(1408..>2122)
                     /locus_tag="PK198_26880"
     CDS             complement(1408..>2122)
                     /locus_tag="PK198_26880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRF42455.1"
                     /translation="ASVRKLLLAEGFETVQTGPNSLLLPRRGNVFRSRAFQSGGVEVQ
                     DLSSQLVAPFLDVAPGMCVVDACAGAGGKTLHLAALMANKGRLIALDTEGWKLEALRK
                     RAKRAGAHCIETRAISSSKIIKRLHGMADRLLLDVPCSGLGVLRRNPDAKWKLNPEQL
                     VQLRRTQQEILERYPAMLRPGGQFVYATCSILPSENEQQVEQFLQSRKEQYRLIKQQT
                     ISPGENNGDVFFIALIEKL"
BASE COUNT          512 a          600 c          518 g          492 t
ORIGIN      
        1 aaaaacattc aacaatgact accatccgcg atacggcttc ggcgctaccg aggtcatcct
       61 gcgctaattt tattctcgct aaggaaaata aacgcctgca tcgtttgcgt ttgatgctcg
      121 cgtatcaaac agtccgtatt cccaaaaaca ttcaatcata cttcgccatg aaaaaagtat
      181 cattttccct tgcctgctcg cttttacttg cctcgtctgc cctcctcact gcacaaaaag
      241 cagagttggg cttcatgggc ggcatttctc tttacaatgg cgatcttgcg cccaaggaat
      301 acattgacta cctcaaagaa gtaaatcccg cattcggcat tttcggaagg gtcaacgtca
      361 acccgtactt cagtgtgagg gcgggcatta cgcaggcgaa agtcaccggc gacgatatca
      421 attcttttcc aagccggggg ctgaacttcc gctccaacat caccgagttg gcccttaccg
      481 gcgagtacag tccttttcaa ttgggaggcc aacgggccaa actggtcaca gcgccttacc
      541 tgtacgcggg ggcgactgtg tatcgtttca acccccaaac tttgtacgac aacaattgga
      601 tagacctgcg ccccttagga accgaagggc aaggactgcc gggctatgaa aagccataca
      661 gcctcacaca aattgcagtg ccgattggcg cgggcatcaa attcaccgtc cggcagacat
      721 ggtcgctggg catagagttc ggcgcgcgca aattgttcaa tgattatttg gacgatatta
      781 cctctgccca agtcaactac ctggatgttt taaccggcaa cggcaccctg gcggccaccc
      841 tcagcaatcc atccatcaaa gagccgaccg atgccaccta ccgacgcggc ggcgaatttc
      901 aggattggta ttatgtaggc ggtgtaacgc tttcgtttcg cctggccgag acccggagca
      961 gatcgggccg gggtttaggt tgccccgaat tttaaaccaa ttgcgccgac gtggcgtttt
     1021 tgagtttaaa tttcaaaaaa atcattctct acaatgagcc atacagccga aatcgcatct
     1081 actcccgtca cactcaccga aggcgcgttg catgaactgg cgcgtattcg caaagagcaa
     1141 aacatacccg ctgaatacgg cctgcgcatc ggcgtcaaag gcggcgggtg ttcgggtttt
     1201 tcttacatac tcggtttcga tgaaccgaaa gacggagacg atactttcca aatcaacgga
     1261 atgaaagtat tgatgcaaaa agcgcacgcc atttatttgc tcggaatgga aattgattgg
     1321 gtggagggcc tcaataatcg gggattctcc ttcaataatc ccaatgccac cgaaacctgc
     1381 ggctgcggca catctttctc ggcgtaatta caatttttct atcaaggcga tgaaaaatac
     1441 atccccgttg ttctcaccgg ggctgatggt ctgttgcttt atcaatctgt actgctcttt
     1501 tcgggattgg agaaattgct ccacctgttg ctcattttca gacggtaaaa tgctgcaagt
     1561 ggcatagaca aactgacctc cggggcgcag catggccggg taacgctcca agatttcctg
     1621 ttgggtacgg cgaagctgga ctaattgctc cgggttcaat ttccatttgg catcggggtt
     1681 ccggcgcaat acccccaagc cggaacaagg cacatccaac agcagcctgt ccgccattcc
     1741 gtgcaggcgc ttgatgattt tggacgaaga gatggcgcgg gtttcaatgc agtgcgcgcc
     1801 ggctcgtttg gcccgtttcc gcagagcctc taatttccag ccttccgtgt ctaatgcaat
     1861 caggcggcct ttgttggcca tcaacgctgc caaatgcaag gttttaccgc ccgccccggc
     1921 acaggcatcc accacgcaca tacccggcgc cacatccaga aacggggcca ccaactgaga
     1981 agacaggtct tgcacctcta ccccacccga ctggaaagca cggctgcgaa acacattgcc
     2041 ccggcgcggc agcagcaagc tgtttggccc ggtttgcacc gtctcaaagc cttccgccag
     2101 cagcagtttg cgtaccgaag ca
//
LOCUS       DAORLF010000618         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28090_length_2109_cov_2.012171, whole genome shotgun sequence.
ACCESSION   DAORLF010000618 DAORLF010000000
VERSION     DAORLF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28090_length_2109_cov_2.012171"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>237
                     /locus_tag="PK198_26885"
     CDS             <1..>237
                     /locus_tag="PK198_26885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42456.1"
                     /translation="RRKILLSILEAFGGTLSRTQMQKLAFLFTRMQATPAYDFVPYRF
                     GCYSFQANQDLAVMQQAGMLSESNLNGFQYWHKQ"
     assembly_gap    238..415
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <416..894
                     /locus_tag="PK198_26890"
     CDS             <416..894
                     /locus_tag="PK198_26890"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRF42457.1"
                     /translation="EQVKAARSTLSEKCLFTIGYEGKSLEQYLNQLLQQNVKLLCDVR
                     KNSYSMKYGFSKSQLQHACEQVGIAFRHIPQLGIESDKRRALDTQRDYDVLFADYERS
                     TLPQNHDYLLELAGLFHQYPRIAITCFEAQVCMCHRGKVAAALQALPDWDIPVGHL"
     gene            914..1753
                     /locus_tag="PK198_26895"
     CDS             914..1753
                     /locus_tag="PK198_26895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42458.1"
                     /translation="MRKKVLITVVTYPLPSRSYDELVCTAGVLEDGSWIRIYPVRFQF
                     LTQAKAPGGFEQYKYSWIELDLKKRTDDFRPESHSPINYDFSDLQVLGKIDTKDHWTL
                     RRQHCLKNVYTNLAQLIAESQEPSNVSLATFKPQAITEFLWETDDREWKPEWQAQLQQ
                     LAFNFGGEHGQTKKKLIRKLPYKFYYKFKDDAGKSSKLMIEDWEIGQLYWNCLQNANG
                     DEATALQKVRQRYWTEFVEDGNKDIYLFLGTTKQWHQRRASNPFVIVGVFYPSQEKQL
                     KLF"
     gene            1863..>2109
                     /locus_tag="PK198_26900"
     CDS             1863..>2109
                     /locus_tag="PK198_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF42459.1"
                     /translation="MLKRLPSGESSFTSIREGDWLYVDKTEDIHRLVTSGKYFFLSRP
                     RRFGKSLTVSTLKALFEGRRELFEGLWIAEHGGWDWSK"
BASE COUNT          510 a          480 c          502 g          439 t
ORIGIN      
        1 accgcagaaa aatcctgctc agcatattgg aagcatttgg gggaacgctt tcccgtaccc
       61 aaatgcagaa gctggcgttc ctctttaccc ggatgcaagc gacgccggcc tacgattttg
      121 tgccgtaccg gtttgggtgc tattcttttc aggccaacca ggatttggca gtgatgcagc
      181 aggccggtat gctgtccgaa tctaatctga atggttttca atattggcac aagcaggnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggag
      421 caagtaaagg cggcgcggag cactctgtct gaaaaatgcc tctttaccat cggctacgaa
      481 ggcaagagtc tggaacaata cctcaaccag ttgctgcaac agaacgtgaa gctgctctgc
      541 gatgtgcgca agaattctta cagcatgaag tatggcttct ccaagagcca gctgcagcac
      601 gcctgcgagc aggtgggcat cgcgtttcga catatcccgc aattgggtat agaaagcgac
      661 aagcgccgcg cattggatac gcagcgcgat tatgatgtgc tttttgcgga ttacgaacgc
      721 agcactttgc cccaaaatca cgactacctg ttggaattgg cgggcttgtt ccatcaatat
      781 ccccgcatag ccatcacctg ttttgaagcg caggtgtgta tgtgccaccg gggcaaggta
      841 gcggcagctt tacaggcatt gcccgactgg gatattccgg tggggcacct ctgaaatcaa
      901 acccacgttg aatatgcgca aaaaagtatt gataaccgta gtgacttatc ccctgccctc
      961 gcgcagctac gatgaattgg tgtgtaccgc aggcgtactc gaagacggca gttggattcg
     1021 catttaccct gttcggttcc agtttctgac ccaggccaag gcgcccggag gctttgaaca
     1081 atacaagtac tcctggatcg aattggattt gaaaaaacga accgatgatt ttcgcccgga
     1141 gagccattcg cccatcaact atgacttttc cgacttacaa gtactgggta aaattgacac
     1201 caaagaccat tggactttgc gccggcaaca ctgtttgaaa aacgtatata cgaatctggc
     1261 tcaactcata gccgaaagtc aggagccgtc caatgtgtcg ctcgccacat tcaaacccca
     1321 agccatcact gagtttttat gggaaaccga cgaccgggaa tggaagcccg aatggcaggc
     1381 gcagttgcaa caattggcgt tcaattttgg aggcgaacat ggccaaacga aaaagaaact
     1441 tatcagaaag ctgccgtata agttttacta caaattcaag gatgatgccg gcaagtccag
     1501 taagctgatg atcgaagact gggaaattgg ccaactgtac tggaattgcc tgcaaaatgc
     1561 aaatggagat gaagcaacag cgctccagaa agttcgacag cgttactgga ctgaatttgt
     1621 tgaagatgga aacaaggaca tatatctttt ccttgggacg acaaagcaat ggcatcaacg
     1681 gcgcgcaagc aatccgtttg tcatagtggg ggtattctac ccaagccagg aaaagcagtt
     1741 gaaattgttt tgaatgcccc gccaagtgcg aagccaaccc gaagtcagcg cctcgctcca
     1801 caaggccgga aggttgttta aagataccgt atgtttcgct atctttgcaa aaaatcgaag
     1861 ccatgctcaa gagattaccg tcgggagaat cgagtttcac ctccatccgc gagggcgact
     1921 ggctgtatgt ggacaaaacc gaggacatac accgcttggt cacttcgggc aagtatttct
     1981 tcctctcccg cccgcgtcgc ttcggcaaat ccttgacggt ttccacgctc aaagcgcttt
     2041 ttgaagggcg ccgggagctg ttcgaggggt tgtggatcgc cgagcacggc ggctgggact
     2101 ggagcaaaa
//
LOCUS       DAORLF010000619         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28145_length_2106_cov_1.837640, whole genome shotgun sequence.
ACCESSION   DAORLF010000619 DAORLF010000000
VERSION     DAORLF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28145_length_2106_cov_1.837640"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>764)
                     /locus_tag="PK198_26905"
     CDS             complement(160..>764)
                     /locus_tag="PK198_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRF42460.1"
                     /translation="AFRKEIKNEYVKVTSGNQARQIQDNIFNYLNNDITVIVYNFIDM
                     MSHARTEMEVLKELAGDEKAYRSLTRSWFLNSPLWAALQRAAERKVQLIIATDHGTIR
                     VNTPSKVVGDRETTTNLRYKVGRNLQYDKKDVLEIRDPEKAGLPRPNVSSAFIFAKDD
                     RFFLYPNNYNHFNNYYRNTFQHGGISLEEMICPVIRLTSK"
     assembly_gap    765..883
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<884..1719)
                     /locus_tag="PK198_26910"
     CDS             complement(<884..1719)
                     /locus_tag="PK198_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF42461.1"
                     /translation="MDKIKILWADDEIDLLKPQLMFLEKRGYEVITVSNGYDALEECE
                     QDKDIDVVFLDESMPGITGLETLSKLKATNPLLPVVMITKNEAEHVMEEAIGSQIADY
                     LIKPVNPNQILLSLKKIIDNKRLVREKTSSDYQQEFRQIFMQINSGLGIEEWADVYRK
                     IINWEIKIDESNSTQMYDILSMQKGEANTEFSKFIVKNYVDWVQRGEGPVMSNQLMRS
                     KVFPHLRDGEPTLFVLLDNLRYDQWKVIEPIITELYRVEEEDFFYSILPTSTQYSRNA
                     IFS"
     assembly_gap    1841..1857
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1866..>2106
                     /locus_tag="PK198_26915"
     CDS             1866..>2106
                     /locus_tag="PK198_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42462.1"
                     /translation="MKSEAQQFIESWERQKAKGKWRFIARSGGIWAGLFLLAQCTLEL
                     AEHSCREAFWSPLTLKHLAVGIIAAIPFGIVQYNLM"
BASE COUNT          412 a          400 c          605 g          553 t
ORIGIN      
        1 gaactcatcc tcgaagaggc cgctcatgtc aatggtgatg gaatatttgc cggagccgtt
       61 tcggttgagg tgcatttctt caaccacatc gaggcagccg gtgaggaaga aagaaacggt
      121 gaagaggaga aggatggtta acttattcat gttggttgtt tattttgaag ttaaacggat
      181 gacgggacaa atcatttctt ccaatgagat gccgccgtgc tggaaagtgt tgcggtagta
      241 attgttgaag tggttgtaat tgttgggata caggaagaag cggtcgtctt tggcgaagat
      301 gaaagccgag ctgacattgg ggcgaggcag gccggctttt tccggatcgc ggatttcgag
      361 tacgtctttt ttgtcgtatt gcaggttgcg gcccaccttg tagcgcagat tggtggtggt
      421 ttcgcggtcg cctaccactt tggagggcgt attgacgcgg atggtgccgt gatcggtggc
      481 gatgatgagc tgtactttgc gttcggccgc gcgttgcaag gcggcccaca gcggggagtt
      541 gaggaaccag gagcgcgtga gggagcggta ggctttttcg tcgccggcca gttctttgag
      601 tacttccatt tcggtgcgtg catgcgacat catgtcaatg aagttataga cgatgacggt
      661 gatgtcgttg ttgaggtagt tgaagatgtt gtcctgaatc tggcgggcct gattgccgct
      721 ggtcactttt acatattcgt ttttgatttc tttgcggaaa gcgcnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaaaaa tggcattgcg
      901 gctgtattgg gtggaggtgg gcaggatgct gtaaaaaaag tcttcttctt ctacgcggta
      961 cagttcggtg atgatgggtt cgatgacttt ccactggtcg tagcggaggt tgtccaacaa
     1021 aacaaacagc gtaggctcgc cgtcgcggag atgcgggaag accttgctgc gcatgagctg
     1081 attggacatg acggggcctt cgccgcgctg cacccagtcc acatagtttt tgacgatgaa
     1141 tttgctgaat tccgtattgg cctcgccttt ttgcatggag aggatgtcgt acatctgggt
     1201 agaattcgac tcgtcaattt tgatttccca gttgatgatt ttgcgataca cgtcggccca
     1261 ttcttcgatg cccaggccgc tgttgatctg cataaaaatc tggcggaatt cctgctggta
     1321 gtcggaggag gttttttcgc ggacgaggcg cttgttgtca atgatttttt tgagggagag
     1381 cagaatctgg ttcggattca ccggtttgat gaggtagtcg gcaatttggg agccgatggc
     1441 ctcttccatg acgtgctcgg cctcgttttt ggtaatcatg actaccggca gcagcgggtt
     1501 ggtggctttt agtttggaga gggtttccag gccggtaatg ccgggcatac tctcgtcgag
     1561 gaagacaacg tcaatgtctt tgtcctgttc acattcttcg agggcgtcgt atccgttgct
     1621 cacggtgatg acttcgtagc cgcgtttttc gagaaacatc aactggggtt tgagcagatc
     1681 aatttcgtcg tctgcccaca atattttaat tttatccatg ctcaagtcag gtggtttgat
     1741 gatgtacgat gacaatgtac gaacaggggg aatagtttag tatggcggga ggtatttttt
     1801 cggcgggcgg agatatatca aaaatcctac ctttggcttg nnnnnnnnnn nnnnnnnaca
     1861 aagccatgaa atccgaagcg cagcaattca ttgagtcttg ggagcgacag aaggcaaagg
     1921 gcaagtggcg gttcatagcc cggtcgggag gtatttgggc gggcctgttt ttattggcgc
     1981 aatgcacctt ggaacttgcc gaacacagtt gccgggaggc gttctggagt ccgctcacat
     2041 tgaagcatct ggcggtgggc atcattgcag ccatcccttt tggaattgtg cagtacaatc
     2101 tcatgg
//
LOCUS       DAORLF010000620         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28240_length_2100_cov_2.445966, whole genome shotgun sequence.
ACCESSION   DAORLF010000620 DAORLF010000000
VERSION     DAORLF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28240_length_2100_cov_2.445966"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..788
                     /locus_tag="PK198_26920"
     CDS             162..788
                     /locus_tag="PK198_26920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42463.1"
                     /translation="MILINYLERFKKTPAFSVREVEQLYPGFERENLLNWQKKGHIIR
                     VRNGWYSVKGRIATEEQLYWVANKIYSPSYVSLETAFAHYGWIPEAVFTITSISTRKT
                     QVFDTQIGHFRYSSVKPSLFFGYRLLVPDGYGTKIAEPEKALLDFLYLRPKIATIADF
                     EGLRLNLDQMRNDIDQNKLEAYCSLFDSQSLTERVRIFKNHLYHVEPA"
     gene            772..1551
                     /locus_tag="PK198_26925"
     CDS             772..1551
                     /locus_tag="PK198_26925"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRF42464.1"
                     /translation="MLSLPELLEAYPVALRVHRSFILREYLQCKILEILFESPFANRF
                     CFLGGTCLRLVHNNSRFSEDLDFDNFQVTESEFGEVSEAIRAGLEQQGYQVEMKQVIR
                     GAYHCYIRFPGLLFEQGISGYREEKILIQLDTEPQYFDFQPESYILNQFDVTTQILTT
                     PLPILLAQKFTALCQRNRSKGRDFFDIVFLLSKSVKPDYSFLELKLGVSNRDQLMEQV
                     RDLCQHLDMEEMVKDVRNFLFFPADEKKIRLFLPLLEQSDF"
     assembly_gap    1705..1877
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1878..>2100
                     /locus_tag="PK198_26930"
     CDS             <1878..>2100
                     /locus_tag="PK198_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42465.1"
                     /translation="LFRSRSLHTTVEAQDSNEWALRHLFFEARNRERLGGSKSLVFGF
                     PLFAVRDEQGRPASQPLFFWNINMEASPNV"
BASE COUNT          477 a          474 c          447 g          529 t
ORIGIN      
        1 tctacaaaca tttgacctct ccgaggtcat tcttggtttt gatgcgtttg ccctgacctt
       61 gttccggttt atacctgctt tctgctatgc ggtgaagtgg gggtatggat aaaaactgta
      121 tctttgcttc ctaaaaacct aattttttag gattaaggga aatgatttta ataaattact
      181 tagagcgatt caaaaaaacg cccgcctttt cagtccggga ggtagagcag ctttaccccg
      241 gttttgagcg ggagaacctc ctcaattggc aaaaaaaagg ccatattatt cgtgttcgga
      301 atggctggta cagtgtgaaa ggccgtattg ccaccgaaga gcaattgtat tgggtggcta
      361 ataaaatcta cagcccgtct tatgtctcat tggagactgc ctttgcgcat tatggctgga
      421 taccggaggc cgtgtttacc atcacctcta tttccacacg taaaactcag gtatttgaca
      481 ctcagatcgg gcactttcgt tactccagcg tgaaaccttc gctttttttc ggctaccgtt
      541 tgttggtgcc ggatggttat gggacgaaaa tagcagagcc ggaaaaagcg ctgctcgact
      601 tcctgtactt gcggcctaaa atcgcaacaa tagccgattt tgaggggctg cgcctgaacc
      661 tcgatcaaat gagaaatgac attgaccaaa ataaattaga ggcgtactgt tcgttattcg
      721 attcacaatc tctgacggag cgcgtccgta ttttcaaaaa ccacctttac catgttgagc
      781 ctgcctgaac tgctggaagc ttatcctgtt gccttgcgcg tacaccgatc attcattctt
      841 cgcgagtact tgcaatgtaa aattcttgaa attttgtttg aaagcccttt tgcgaaccgc
      901 ttttgcttcc tgggcggcac atgcctgcgc ttagtacaca ataattcccg attttccgag
      961 gacctggatt tcgacaattt tcaagtcact gaatccgaat tcggagaggt gtccgaagct
     1021 atccgagccg gactggaaca acaggggtat caggtagaaa tgaagcaggt catacgggga
     1081 gcatatcatt gttatatccg ttttccagga ctgttgtttg agcaagggat cagcggttac
     1141 cgcgaggaaa aaatcctcat tcaactcgac acggagcctc aatactttga ttttcaacct
     1201 gaaagttata tcctgaacca atttgacgtg actacccaaa tccttaccac gcccttgccc
     1261 atccttctgg cgcaaaaatt cacagcactt tgccaacgca acaggagcaa gggccgcgat
     1321 tttttcgaca tcgtatttct cctatctaaa tcggtgaaac ccgattattc atttctggaa
     1381 ttgaagctgg gggtctccaa tcgcgatcaa ttgatggaac aggtacgaga cctttgccaa
     1441 catctcgaca tggaggaaat ggtaaaagat gtccgcaact ttttgttttt cccggcagac
     1501 gaaaaaaaga tacggctgtt tttgcctctt ttggaacaat cggacttttg acgaaacatc
     1561 tccaattgtt gctcccgccc aatgaaggtg gaactgcgtc gttgtttgag ccggtcttga
     1621 agcgatatag ccatatctca caggcctgcc ttctggatac aagaacgcga aagccgcagc
     1681 tatttctcct cactgctacc aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnngct gtttcgctcc cgctcgctcc atactacggt agaggcgcaa
     1921 gattccaacg aatgggcgct gcggcacctg ttcttcgagg cgcgcaaccg cgaacgcctc
     1981 ggcggcagca agagtttggt tttcggattc cccctgtttg ccgttcgcga cgagcagggc
     2041 cggccggcct cgcagccctt gtttttctgg aacatcaaca tggaggccag ccccaacgtg
//
LOCUS       DAORLF010000621         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28503_length_2085_cov_1.944828, whole genome shotgun sequence.
ACCESSION   DAORLF010000621 DAORLF010000000
VERSION     DAORLF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28503_length_2085_cov_1.944828"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..562
                     /locus_tag="PK198_26935"
     CDS             113..562
                     /locus_tag="PK198_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRF42466.1"
                     /translation="MNLEQRIKQDLAEAMKARDEASKRGLRAVLAAIQLAKTDGSGEA
                     IEGDREIKLLQKLVKQRRDSVEIYQKGNRPELAAVELEEIAVIEKYLPQQMSEAELEI
                     ALREIIAQTGATSARDIGKVMGAANKALAGKAEGKMIAAVAKRLLEA"
     assembly_gap    790..799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1031..>1838)
                     /locus_tag="PK198_26940"
     CDS             complement(1031..>1838)
                     /locus_tag="PK198_26940"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRF42467.1"
                     /translation="IVEINAVDAAGVHPLLLAVGSERYMPFRERKPEEILTQANHILG
                     SGQTSLAKFLFIAAQGDAPELNTHDMASFFQHVLSRVDWRRDLHFHTRTTIDTLDYSG
                     TGWNEGSKVVVACCGPQRRTLTDTLPDGFSLPAGFSRPRFVMPGVLAVQAPAFAEYGA
                     YADCTTLTEHLRQAQTEGIPLIVLTEDSDFLAAALNNFLWVTFTRANPSHDLHGVASF
                     VEHKHWGCRGPLVIDARIKPHHAPPLVTDAEISKRVDALFAKGGSLQKWG"
     assembly_gap    1839..1858
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1859..>2085)
                     /locus_tag="PK198_26945"
     CDS             complement(<1859..>2085)
                     /locus_tag="PK198_26945"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRF42468.1"
                     /translation="ADFVITGIISKDIKKPEGPFGDHLGYYSLAHDFPVMEVEKVYYR
                     KNAIWHFTVVGRPPAEDSSFGYLIHKVVEKLT"
BASE COUNT          501 a          543 c          543 g          468 t
ORIGIN      
        1 tcttatctaa tcctttggcc taaagaaaat gaacatttat ttttgcccca ctacatagct
       61 cgcggctcgc agctttttca tgcttatcat tcacttcaat aaccgttcaa ccatgaacct
      121 cgaacaacgc atcaaacaag acctggcgga agccatgaaa gcccgcgacg aagcctccaa
      181 acgcggcctg cgcgctgtgt tggcggccat tcagttggcc aaaaccgacg gcagcggcga
      241 agccatagaa ggcgaccggg agatcaaact gctccaaaag ctcgtcaaac aacgccgcga
      301 ctcggtggaa atttaccaaa aaggcaatcg gcccgaactg gctgccgtgg aattggagga
      361 aattgccgtc attgaaaaat acctgccgca gcaaatgagt gaggctgagt tggaaatcgc
      421 tctccgcgag atcatcgcgc aaaccggcgc tacctccgcc cgcgacatcg gcaaggtgat
      481 gggcgcggcc aacaaagcct tggccggcaa agccgaaggc aaaatgattg cggctgtcgc
      541 caagcgcctg ttggaggctt gaggcatttc aacaatatgg aaccggaagg ccggaatatc
      601 tcatccaata ccataagggc tgcggtattc cggctttcgt tttaatctta tattacaaaa
      661 aaattaaata tattttgcca ttccaataaa agctatatct ttgccccgaa atcattccca
      721 aaaccgccgg agcattcttt tgaccagctc caatcatctg cataccaaca acaaaacagt
      781 tctgtgagan nnnnnnnnnc ccgcctgcgg cgaggattct tttatatggt cattcactat
      841 tctatgtgaa tgcaaaggct gtttgttttc aattttgaca cactatttga tccaagcctg
      901 atatacaggc gatatgcaat gctccggcac ggcgggaatc gggatgtact ctggatgtat
      961 ttatggtaat ccaagcgttg gtttaacaaa aaacgaaaga ggcgtttcgg cccgaccgtg
     1021 tccgggtttt tcaaccccat ttttgcaacg aacctccttt ggcaaataag gcatctaccc
     1081 gtttcgatat ttcggcatcc gttaccaatg gcggcgcgtg gtgcggcttg atgcgcgcat
     1141 ctatgaccaa aggcccacgg catccccagt gtttatgctc cacgaagctg gccacgccgt
     1201 gcaagtcgtg gctgggattg gccctggtga aagtcaccca aaggaaattg ttgagcgcgg
     1261 ccgccaaaaa atcgctgtct tcagtgagta caatgagcgg aatgccctcc gtttgtgcct
     1321 ggcgcaagtg ctcggtcagg gtggtgcaat cggcgtaagc tccatattcg gcaaaagccg
     1381 gcgcctgaac ggccaaaacg cccggcatca caaagcgagg tcgtgagaaa cctgccggca
     1441 gactgaatcc gtcgggtaat gtgtcggtca gtgttctgcg ttgcggcccg cagcaagcca
     1501 ccactacttt agagccttcg ttccagccgg tgccggaata gtcgagcgta tcaatggtgg
     1561 tgcgggtatg gaaatgcagg tcgcggcgcc agtccacacg cgacaacacg tgctgaaaga
     1621 agctggccat atcgtgggtg ttcagttccg gcgcgtctcc ctgcgctgcg atgaaaagga
     1681 atttggccaa agaggtttgc cccgaaccga gaatgtgatt ggcttgggtg agaatttcct
     1741 ccggtttgcg ttcccggaag ggcatgtatc tctcgctgcc cacggccagc aagagcgggt
     1801 gtaccccggc ggcgtccacc gcgttgattt ccacgatgnn nnnnnnnnnn nnnnnnnngt
     1861 cagtttttcc accactttgt gaatgaggta gccgaagctg gagtcctcag ccggcgggcg
     1921 gcccactacg gtgaaatgcc agatggcgtt tttgcgataa tacacttttt cgacctccat
     1981 gacggggaaa tcgtgggcaa ggctgtaata accgaggtga tcgccaaagg ggccttccgg
     2041 ctttttgatg tctttgctga taatgcccgt gatgacaaaa tcggc
//
LOCUS       DAORLF010000622         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28533_length_2083_cov_2.273669, whole genome shotgun sequence.
ACCESSION   DAORLF010000622 DAORLF010000000
VERSION     DAORLF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_2083_cov_2.273669"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="PK198_26950"
     CDS             <1..>1111
                     /locus_tag="PK198_26950"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF42469.1"
                     /translation="GTGCAPAEVVFENQSSQNATAFAWSFPGGTPAESSEASPNVSYP
                     QPGTYTATLIVSNAAGADTISQTLTVGGLPLGTFSAQVNGPQVSVQANSSNATVLNWN
                     FGDGSQSSEPNSTHTYSADGDYLITLTLSNACGETILTDLVTIFTALPVAGFSAGQRE
                     GCAPLEVSFSNESSANAQSFLWQFPGAQPETSSEINPTVSYAEPGIYTVSLTAINSNG
                     GSAITRMDYIVVRGLPVGGFTYEITGSEVAFQTTTSQEPGVTYHWDFGNGHTSNLASP
                     THDFGSPGGEYLVTLELRNECGSTITAETLLIIVSRTGEAISGNGWSVFPNPASGQVV
                     VAKAQSSMGQPFDWVLRDVLGHTLKRVRLDTVGVSV"
     assembly_gap    1112..1228
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1342..1608
                     /locus_tag="PK198_26955"
     CDS             1342..1608
                     /locus_tag="PK198_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRF42470.1"
                     /translation="MKKGIHPESYRTVVFKDFSCDEAWLSRSCAPTKEKIMWEDGNEY
                     PLIKLEISSNSHPFFTGKMKFVDTAGRIDKFNKKFANTRFNKPS"
     gene            1703..>2083
                     /locus_tag="PK198_26960"
     CDS             1703..>2083
                     /locus_tag="PK198_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRF42471.1"
                     /translation="MNIILFDSEVRGELLPFTYLRPVCEIRIGILTIREKWERRMNAS
                     VSYITQDYLAGKFPIDYGSENYLINGSVLPSDQLVQLLREMEFGQAYLKGEELIAAKL
                     DGDQMERLVHDEDFGELRGSVVLDT"
BASE COUNT          465 a          534 c          512 g          455 t
ORIGIN      
        1 gggcacaggc tgcgcgcctg ccgaggtcgt ttttgaaaat cagtcgagcc aaaatgcaac
       61 ggctttcgcc tggagcttcc ccggtggaac gcccgccgaa tcctcggaag cctcgcccaa
      121 cgtgagctat ccgcagcccg gtacctatac cgccacgctc atcgtgagca atgccgccgg
      181 ggccgatacc atcagtcaga cgctgacggt gggaggtctg cctttgggta ccttcagcgc
      241 acaggtcaac ggtccgcagg tttccgtgca ggccaattcc tccaacgcaa ctgtcctgaa
      301 ttggaatttt ggcgacggca gccaaagctc cgaaccgaac tcgacgcaca cctattctgc
      361 cgacggggac tacctcatca cgctgacgct gagcaatgcc tgcggtgaaa ccatcctgac
      421 cgatctggtg acgatattca ccgcacttcc cgtagccggg ttcagcgccg gccaacgcga
      481 gggctgcgcg cccttggagg tttcgttcag caatgaatct tctgccaatg cgcaaagctt
      541 cctgtggcaa ttccctggcg ctcagcctga aacctcgtcg gagatcaacc caacggtaag
      601 ttacgcagag ccaggtattt atactgtttc gcttactgct atcaacagca acggaggctc
      661 agccattacg cgtatggatt acatcgttgt tcgcgggctg ccggtcggcg gatttactta
      721 tgagattacc ggctcggaag tggcgttcca aaccactacg tcacaagaac ccggcgtcac
      781 ttatcactgg gatttcggca acggccacac ttctaatctg gcatcgccca cacacgattt
      841 cggcagtccg ggcggagagt atttggttac actggaactc cgcaacgaat gtggcagcac
      901 cataacagcc gaaaccttgc tcatcatcgt cagccgcaca ggcgaggcaa tttccggaaa
      961 cggatggagc gtttttccca atcccgcatc ggggcaggtg gtggttgcca aagctcaaag
     1021 cagcatgggg cagccttttg actgggtact gcgcgatgtg ttggggcata ccctcaagcg
     1081 cgtcaggttg gatacggtcg gcgtctcggt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tcatatggag gccattgtag ggggaggttt
     1261 aaaaaaaatc tgttgaaggc ttgcaaattt tgcagataag cgtaattttg caccctgttt
     1321 taaaaaaaga agaaaagcgc catgaaaaaa ggtatccatc ctgaatctta ccgcaccgtt
     1381 gtttttaagg acttctcttg tgatgaagcc tggttgtcac gttcttgcgc tcccacgaag
     1441 gagaaaatta tgtgggaaga cggcaatgaa tatccgctga ttaagttgga aatttccagc
     1501 aactcgcacc cgttctttac cggcaaaatg aagtttgtgg ataccgccgg tcgtattgac
     1561 aagttcaaca agaagtttgc caatacccgc ttcaacaagc cgagctgatt ttttggggat
     1621 acctttcgcc tccctaagct cctgaacaac tgccgggcgc gcttgcttta cgacatcaat
     1681 accccgatgt atcaaccggg atatgaatat tatactgttc gatagtgaag tcagaggtga
     1741 actattgccg ttcacctatt tgcggcccgt ttgcgaaatt cgcatcggca ttttgactat
     1801 tcgggaaaag tgggagcgaa ggatgaacgc ctctgtttcc tacattaccc aagactacct
     1861 cgccgggaaa ttccccatag actacggttc cgaaaattac ctcatcaacg gctctgtgct
     1921 gccctccgac caattggtgc aactgctgcg cgagatggaa ttcgggcagg cttatctgaa
     1981 aggagaagaa ctgattgccg ccaaattgga tggcgatcag atggaacgct tagttcacga
     2041 tgaagacttc ggcgaactgc gcggctccgt cgtgttagat acc
//
LOCUS       DAORLF010000623         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28580_length_2080_cov_2.306667, whole genome shotgun sequence.
ACCESSION   DAORLF010000623 DAORLF010000000
VERSION     DAORLF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28580_length_2080_cov_2.306667"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK198_26965"
     CDS             <1..589
                     /locus_tag="PK198_26965"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRF42472.1"
                     /translation="LYQRLNAEEQLKFRQRVSLFMMAQQYLPQGVEKVSPDVEAAVAA
                     SAVTLTMGKKDFLFPKYENIVLYQHPFPSPQYPELFHISETQDEDGVLLFSIQHLMLG
                     LLQPDQYLHIGLYEYAKVYMRSYPFARYPQVEEAHWPVLERISGFSRTAIETYLNVPD
                     ISLPALATVFYFTQPEVFRSEWPEVFEQFSVVYQG"
     gene            complement(580..1938)
                     /locus_tag="PK198_26970"
     CDS             complement(580..1938)
                     /locus_tag="PK198_26970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF42473.1"
                     /translation="MKTKFFLFIALCFAVGQGFAQQRAVLDKVVAVVGSELILLSELE
                     EQHSYLESQRGVLPPDARCAILDNIMLSKLLLNQAKLDSVEVSDAEVEDQINARIERI
                     LTYMNNEVSAFEDYYGQSIAEVKDQMREDLKNQMLTERMEGQIKSGITITPSEVKAFF
                     NKIPRDSLPYFNAEVEVGEIVYKPKVNEEQRQIAMKKLESLRQRIVEGGEDFAALASK
                     FSDDGGSARAGGELGWTKRGVFVPAFEAAAYKLEKDEISPIVESEFGFHFIQLLDRRG
                     NSIRTRHILIKPEITTEDMQLAYNTLDSVRQLILSDSMSFSLAVKRFSHKEVQSYNND
                     GRMVNSATQNTFFQINELEPDIYFAIDTLKVGGITKPFEFTGPDGETYYRIAVLQSKT
                     APHRANLSQDYSKIQKAAVEAKKSEHISKWVIEKMSTTYISIDPSFKDCRELAKWRAY
                     QP"
BASE COUNT          455 a          515 c          557 g          553 t
ORIGIN      
        1 cctgtatcag cggctgaatg cggaggagca actgaagttc cggcagcggg tgagcctgtt
       61 tatgatggcg cagcaatatc tgccgcaggg cgtagaaaaa gtatcgcccg atgtagaggc
      121 agcagtggcg gcttcagcgg tgacgctgac aatgggcaaa aaagactttc tgtttcccaa
      181 atacgaaaac attgtattgt atcagcatcc gtttccatcg ccgcagtacc ctgaactttt
      241 tcacatatcg gaaacacagg acgaggacgg ggtcttgctt ttttcgatac aacacctgat
      301 gctgggcctg ttgcaacccg atcagtatct gcacattggc ctgtacgaat acgccaaagt
      361 atatatgcgc agttatccct ttgcccggta tccgcaggtg gaggaggcgc attggcctgt
      421 attggagcgc atcagcggtt tttcccggac ggcgatagag acgtatctca atgtgcccga
      481 tatttcactg ccggcgctgg ctacggtttt ttactttacg caaccggagg tgtttcgcag
      541 cgagtggccg gaggtatttg agcaattcag cgtcgtatat caaggctgat aggccctcca
      601 tttggccagt tccctgcaat ctttaaaaga cggatcaata gagatgtagg tggtgctcat
      661 tttttctatg acccacttgg agatgtgctc gctcttcttg gcctcaacgg cagccttctg
      721 aattttggaa taatcttgcg aaagattggc ccggtgcggc gctgttttgg attgcaaaac
      781 ggcgatgcgg tagtaggttt cgccgtccgg cccggtaaac tcgaagggct tggtgatgcc
      841 gcccaccttg agcgtatcaa tggcgaaata gatgtcgggt tccaactcgt tgatctggaa
      901 gaaagtattc tgcgtggccg aattgaccat gcgcccgtcg ttgttgtagc tctgcacttc
      961 tttgtgcgag aaacgtttca ctgccaggga aaagctcatg gaatcgctga gaatcaactg
     1021 gcgcaccgaa tcgagggtat tgtaagccaa ttgcatgtct tctgtggtga tttccggttt
     1081 gatgagaatg tgccgggtgc gaatgctgtt gcccctgcgg tcgagcaact gaataaagtg
     1141 aaaaccgaat tccgactcca caatgggcga tatttcatcc ttttccaact tgtaggcagc
     1201 cgcttcaaaa gcaggtacga acacgccgcg tttggtccaa cccagttcgc cgccggcgcg
     1261 ggccgagccg ccgtcgtctg aaaacttaga ggcgagcgct gcaaaatctt ctccaccttc
     1321 cacgatgcgt tgccgcaggc tctccaattt tttcatagct atctgtcgtt gttcctcgtt
     1381 tactttgggt ttgtaaacga tctcgcccac ctccacttct gcattgaaat aagggagact
     1441 gtcacggggt attttgttaa aaaatgcctt tacttctgag ggtgtgatgg tgatgccact
     1501 tttgatctga ccctccatgc gctcggtgag catctggttt ttcaggtctt cccgcatctg
     1561 gtctttcacc tcggcaatgc tctggccgta gtaatcctca aaggccgaca cttcgttgtt
     1621 catgtaggtg agaatgcgtt cgatgcgcgc attgatctgg tcttccacct ctgcatcgga
     1681 tacctctacg ctgtccaatt tggcttggtt gagcagcagt ttggagagca tgatgttgtc
     1741 taaaatagcg caacgggcat ccggaggcag tacgccacgc tgtgattcga ggtaggaatg
     1801 ttgctcttcc agttcagaaa gcaggatcaa ctcgctgccc accacggcca ctactttgtc
     1861 gagtacggcg cgctgttgtg caaatccctg cccgacggcg aaacaaagcg caatgaaaag
     1921 aaagaatttg gttttcatat tttgaagtgt cttgttgttt ttaaaattca aggtttcggg
     1981 tttcatccgc cgaaccacgc cacaggcgtg ggaaggtggg tttcgggttt aaggtttcgg
     2041 gttggaaccg cccaccgcat accgaaaacc ccgcgccatg
//
LOCUS       DAORLF010000624         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28596_length_2079_cov_2.288538, whole genome shotgun sequence.
ACCESSION   DAORLF010000624 DAORLF010000000
VERSION     DAORLF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28596_length_2079_cov_2.288538"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1792)
                     /locus_tag="PK198_26975"
     CDS             complement(<1..>1792)
                     /locus_tag="PK198_26975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42474.1"
                     /translation="AVTDSLEIVDFDCTDPATFPLPANTRPIAALQYGDVVTVGGFQM
                     RITEVTASEETPGGWDGTGQIRINWMAGKRFICKFEGLMVNTDKQVIDGEVYAFTKGL
                     ESIPDWKSPEQILEELAEMPVENMATICGTDSTAVSTGTPAGGTGAGASTTTGTTTAT
                     PPADSIPPAFTPYPDTSVYANPFATYEPDSLPFNAGLNAGANTLPFILGGYPNAIVID
                     NIVFRPQGATLNAYFTAGLPGTSGNTPDSTAASTPATVPDSTSTAVAADSTATEEPAN
                     RYVAFQAIDVAFHPGGLLGEARMQLMTNHSAKWSSKIRLTVLQSPNSYVSWDCNGLRG
                     ISIDGKVEFCPSLLVALHPDSLTVAPDDFVTGTFVAAMPKWGQFVADMSITPFEIPRL
                     RDWGWEVQNMVFDFSDEMTPASVVFPDGYSHADVNPDSLAGDLNPRWTGFYLRTARVK
                     VPERFYGSRGAPVQGVASDLIIDQTGFTGNVTGINVLSRDRGRVGTWEFSLDTLQIGI
                     QQNQFHHVGFVGTTKVPSFENAFRYTCLIQPDSAYRFSVGIGEDTMRLSAWKADVELY
                     ENTRIELDYDVSEREFSARAYLYGMASMKPT"
     assembly_gap    1793..1807
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1808..>2079)
                     /locus_tag="PK198_26980"
     CDS             complement(<1808..>2079)
                     /locus_tag="PK198_26980"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF42475.1"
                     /translation="PEFFTYGEACRAPDNIAVETQNESSIRVEWTPRPGFNRYVVRYR
                     ERDTPGANWYEDETLLTTHNLENLIDATAYEIQVQTLCNGDYQGPFS"
BASE COUNT          405 a          532 c          677 g          450 t
ORIGIN      
        1 ggtgggcttc atggaggcca tgccgtagag gtaggcgcgg gccgaaaact cgcgctcgct
       61 cacgtcgtag tccaattcga tgcgggtatt ttcgtacaat tccacatcag ctttccaggc
      121 gctcaggcgc atggtgtctt cgccgatgcc caccgagaag cgataggccg agtcgggctg
      181 aatgagacag gtataacgga aggcattttc aaaagagggc actttggtgg tgcctacaaa
      241 accgacgtgg tgaaactggt tttgctgaat gccgatttgc agagtatcca acgaaaactc
      301 ccaggtgccc acgcggccgc ggtcgcggga gagcacgttg atgccggtga cgttgcccgt
      361 aaagcctgtt tggtctatga tgagatcaga agcgaccccc tgtaccggtg cgccgcgcga
      421 gccgtagaag cgctccggca ctttcacgcg ggcggtgcgc aggtagaagc ccgtccagcg
      481 gggattgagg tcgccggcca gcgaatcggg gttgacatcg gcgtgggagt agccgtcggg
      541 gaagaccaca ctggcgggag tcatttcatc gctgaagtca aagaccatgt tttgcacttc
      601 ccacccccag tcgcgcaggc gaggaatttc aaacggggtg atgctcatat cggccacaaa
      661 ctgcccccat ttgggcatgg cggccacgaa ggttccggtg acgaaatcgt cgggggccac
      721 ggtgagcgag tcggggtgta atgccaccaa caggctggga caaaattcca ccttgccgtc
      781 tatgctgatg ccgcgcaagc cgttgcaatc ccaactgacg taagaattgg ggctttgcaa
      841 aacggtaagc ctgattttac tgctccactt ggccgagtgg ttggtcatca gttgcatacg
      901 cgcctcgccg agcagaccgc cggggtggaa cgcaacgtct atggcctgaa aggcgacata
      961 gcggttggca ggttcttcgg tggcggtaga atcggcggcg actgccgtgg aggtgctgtc
     1021 gggtacagtg gccggagtag aggcagccgt tgagtcgggg gtgttgccgg atgtgccggg
     1081 caaacctgcc gtgaagtagg cattcagcgt agcgccctgt ggacggaaca cgatgttgtc
     1141 tatcacgatg gcattgggat aaccgcccaa aatgaaaggc agggtgttgg cgccggcgtt
     1201 caagcctgca ttaaagggca gagaatcagg ctcgtaggtg gcaaatggat tggcatatac
     1261 gctggtgtcg gggtagggtg tgaaagcagg agggatagag tctgccggcg gagtggcggt
     1321 ggttgtgccg gtggtcgtgc tcgctccggc gcccgttcct cctgcgggtg ttcctgtact
     1381 cactgctgtt gaatcagtgc cgcaaatggt ggccatattt tctaccggca tttcggcgag
     1441 ttcttccaga atttgctccg gcgatttcca gtcggggata ctttccaatc ctttggtaaa
     1501 ggcatatacc tcgccgtcaa tcacctgttt gtcggtattg accatcaggc cctcaaattt
     1561 gcaaataaag cgcttgccgg ccatccagtt gatgcgaatc tggccggtgc cgtcccatcc
     1621 gccgggcgtt tcttcggatg ccgtgacctc ggtgatgcgc atctgaaaac ctcccaccgt
     1681 gacgacatcg ccgtattgga gagcggcaat ggggcgggta ttggcgggca gcgggaaggt
     1741 ggcggggtcg gtacagtcga aatctactat ttccaaagag tcggtgacgg cgnnnnnnnn
     1801 nnnnnnngaa aacggcccct gatagtcgcc gttgcacagg gtttgcacct gaatttcata
     1861 agcggtggcg tctatgaggt tttctaagtt gtgggttgtc agcagggttt cgtcctcgta
     1921 ccagttggcg ccgggcgtgt cgcgttcgcg gtaacgcacc acataccggt tgaagccggg
     1981 ccggggcgtc cattccacgc ggatgctgct ctcgttttgc gtttccacgg cgatgttgtc
     2041 gggggcgcgg caggcttctc cgtaggtgaa aaattcagg
//
LOCUS       DAORLF010000625         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28646_length_2075_cov_1.943069, whole genome shotgun sequence.
ACCESSION   DAORLF010000625 DAORLF010000000
VERSION     DAORLF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28646_length_2075_cov_1.943069"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..142)
                     /locus_tag="PK198_26985"
     CDS             complement(2..142)
                     /locus_tag="PK198_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42476.1"
                     /translation="MLKELANGEYVALAKVFLKDAKEPLVLQRYISLWESGPRLDNKV
                     EH"
     assembly_gap    319..332
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<333..1045)
                     /locus_tag="PK198_26990"
     CDS             complement(<333..1045)
                     /locus_tag="PK198_26990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42477.1"
                     /translation="MKKTIFLFATLITLTTLSAQQTPQKMSYQAVIRDNSGVLVANSV
                     VGIRISILEGSASGPAVYVETHTVTTNTNGLASLEIGGGIPISGNFSLINWPVHDFYL
                     KTETDPNGGSAYNIVGTSQLLSVPYALYSNIGGLHVKKLHTEDELNLTRTTTTGPNVP
                     GSSTDIGDITQYKGLVLEWRIKDSREVFHQFIYLSQYDKAVILEIIPTPEDYPRRYTT
                     ILSDNFGTLMWLGVSIQGTR"
     gene            complement(1050..1526)
                     /locus_tag="PK198_26995"
     CDS             complement(1050..1526)
                     /locus_tag="PK198_26995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42478.1"
                     /translation="MKQKIIIFIFLLTGSGLIANAQQTVAGAGGDAFGNGGSAAYTVG
                     QITYTTHAAPTGSMVQGIQIANEVYTVNIQEAENMSAILLYPNPATDALMLKLNLPLQ
                     REMFFRLYDPQGKLLQQTPITSDLMLIDVAGLPGGAYFIAVFSADSKVQTLIIIKQ"
BASE COUNT          528 a          480 c          476 g          577 t
ORIGIN      
        1 ctcaatgctc gaccttatta tccaaacgag gaccactctc ccatagcgaa atataccgtt
       61 gcagcaccag cggctctttg gcgtctttca ggaatacctt ggcaagcgcg acatattctc
      121 cattggccaa ctctttgagc atgaactggt ctttggcgct gcggcggtat tgcaccggat
      181 gtggataagc ccaaccgcac cgggaggcct tcctgctgcg gatgaaaagc cttgggattc
      241 gaggcaaaaa tcatagtatc gcaaatatcc gttgcagtct tgtcgtgggg cttgcgactg
      301 agttggttga tatgtaaann nnnnnnnnnn nncgggttcc ctgtatggaa acgcctaacc
      361 acatcagggt gccgaaatta tctgacaaaa ttgtggtata tcgcctggga taatcttccg
      421 gcgtgggaat aatctccaga atcacggctt tatcgtattg agacaaataa atgaactggt
      481 gaaaaacttc ccgactatct ttaatacgcc attcaaggac taggcccttg tactgggtaa
      541 tatctcctat atcggtcgaa gatcccggta cattcggacc tgtagtggtc gtacgagtta
      601 aattcaactc atcctcggtg tgtaattttt taacatgcaa cccgcctatg ttggaataca
      661 aggcataagg cacactcagc aattggcttg ttcccacgat attgtacgca ctgccgccgt
      721 tcggatcggt ctccgttttt agataaaaat catgtactgg ccagttaatc aaagaaaaat
      781 tcccacttat ggggatgccc ccgccgattt cgagactggc caaaccgttg gtgttggtgg
      841 taacggtatg cgtttccaca tacacggccg gccctgatgc ggagccttcc agaatactga
      901 ttcggatgcc gactaccgaa ttggcaacca gaacgcctga attgtcccga atgacggctt
      961 ggtaactcat tttttggggt gtttgctggg cagataaggt tgtcagagtg atgagtgttg
     1021 cgaacagaaa gatagtcttt ttcatgacgt tactgtttaa tgattataag ggtttgtacc
     1081 tttgaatcag ctgaaaagac agcaataaaa taggcgccgc ccggcagtcc ggccacatca
     1141 atgagcatta ggtccgaagt aattggcgtc tgctgtaaca acttaccttg aggatcatat
     1201 aaccggaaaa acatctcccg ctgtaacggc aaattaagtt tcaacatcag ggcatcggta
     1261 gcaggattcg gataaaggag tattgcagac atattttctg cctcctgaat atttaccgtg
     1321 tacacctcgt tggcgatctg gattccttgc accatactgc cggtcggggc ggcatgggtg
     1381 gtatatgtaa tttgccccac agtgtaggcg gcagaacctc cgttgccaaa agcgtcgcct
     1441 cctgcacctg caacggtttg ctgggcattt gcgatcagac ctgaacctgt tagcaggaat
     1501 atgaagatga ttattttctg tttcatgtcc tgatgaagtt tgattgctgt tgggtaaatg
     1561 agatcatgtt gatatcgctg agatcggtta tgtccatgat tctgtttttt taaaattgca
     1621 tccgtataat attgacggaa accgttctac atcatacatt ctcaaaaaga aataccccac
     1681 acccgaccaa cccagcatca aatccggcag gtcgtattcg gtctggatgc cgctgcccat
     1741 gcgttgatta cttcataatt tgtttccatg atgatggttt ttatttaacc agattgcttg
     1801 tgtcgccgca ggcgcttgct tcttcatacc gcactcataa attttattga ctccctgcca
     1861 gataatagta agctgtccat gctcttttct ccatcaagtt gccaaccaag tgcatgcgaa
     1921 tgcactcata agatatagct acatgggata gaactaagat tttaacacct tgaaatttcc
     1981 aacacttctt ccaaatcgta gatagctggg ggcacatgct ccagcttcct gctgctgccg
     2041 gtcaggtagg tgccgttttt taaaaaagca aaatg
//
LOCUS       DAORLF010000626         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28678_length_2073_cov_2.294351, whole genome shotgun sequence.
ACCESSION   DAORLF010000626 DAORLF010000000
VERSION     DAORLF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28678_length_2073_cov_2.294351"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..245
                     /locus_tag="PK198_27000"
     CDS             33..245
                     /locus_tag="PK198_27000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42479.1"
                     /translation="MSTYDQLIEKGRAEGIEKGIEKGIEKGIEKGKIEVALNAFANGL
                     SIQLIANITEMEEAEVTRILKEHGMM"
     gene            complement(266..1012)
                     /gene="kdsB"
                     /locus_tag="PK198_27005"
     CDS             complement(266..1012)
                     /gene="kdsB"
                     /locus_tag="PK198_27005"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRF42480.1"
                     /translation="MKIAGIIPARYASTRFPGKPLADIGGQSLIERVYRQVRQTTCIQ
                     EVVVATDDERILRHVLDFGGQAVMTRSDHASGTDRCAEVAEALSGFDIVVNIQGDEPF
                     IRPEQIEAALEPLLHSSDAGIATLAKKMDRREDVFNPNVVKVVFNERGRALYFSRSPI
                     PYLRGIPEAEWPGDIDFYKHIGLYAFRREVLSELTHLPMSAHERAESLEQLRWLDAGY
                     SIAVSVTPFETIGVDTPEDVARALLEGLGQ"
     assembly_gap    1100..1113
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1114..1454)
                     /locus_tag="PK198_27010"
     CDS             complement(<1114..1454)
                     /locus_tag="PK198_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRF42481.1"
                     /translation="MENYLMKIELRRLSGDYHLQAVNEDGNTVETDASPAIGGTGKGM
                     RPMQMLLSALGACSSIDVILIMKKKRQELRDIKVSVTGEREEGAIPEVFKTIHVHYDL
                     YGPLDAAKAERA"
     gene            1486..>2073
                     /locus_tag="PK198_27015"
     CDS             1486..>2073
                     /locus_tag="PK198_27015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF42482.1"
                     /translation="MNQITIGLIFLGCAAAAGYALLCFWVVRNWRRLPEWTVPEAFVP
                     HTSVSVVIPARNEAAGIEACLRSVFQQNYPPHLLEIIVVDDHSEDDTVAIVGSLNLPN
                     LRLLRLADFVQPGATTSFKKKAIKTAIAHARGELIVTTDADCTAPPDWLPAIVSLYEQ
                     RRPKAILASVGLAPAANKMERFQALDMAGLMLCSGA"
BASE COUNT          482 a          572 c          527 g          478 t
ORIGIN      
        1 ccaaatattc aggacaaaaa aattcaacag ctatgtcaac gtacgatcaa ctcattgaaa
       61 aaggcagagc cgagggcatt gaaaaaggca ttgaaaaagg cattgaaaaa ggcattgaaa
      121 aaggcaaaat cgaagtagca ctcaatgcgt ttgccaatgg gctttctatt caattgattg
      181 ccaacatcac cgagatggaa gaagctgaag taacccggat tttgaaagaa cacggcatga
      241 tgtaatgcct ccggtttatc aggttttact gacctaaccc ttccaataag gcccgcgcca
      301 cgtcctcagg cgtatccact ccgatggttt caaacggcgt cacggagacg gcaatggaat
      361 agcctgcatc taaccagcgc aattgctcca gcgattctgc ccgctcatgg gcgctcatgg
      421 gcaagtgggt cagttccgac aacacttccc tccgaaaagc ataaagcccg atgtgtttgt
      481 aaaaatcaat gtcccccggc cactccgctt cgggtatgcc gcgcagatat ggaatggggc
      541 tgcgactgaa atacagtgcc cgcccgcgct cgttgaagac tacttttact acattgggat
      601 tgaaaacatc ttcgcggcgg tccatttttt tggctaaagt ggcaatgccc gcatcggaag
      661 aatggagcag cggctccaaa gctgcttcta tctgctcagg ccggatgaag ggttcatcgc
      721 cctgaatgtt caccacaatg tcaaaccccg acaaggcctc tgccacttct gcacagcggt
      781 cggtacccga tgcgtgatcg ctgcgcgtca tcacagcctg cccgccaaaa tcgagtacat
      841 gccgcagaat gcgctcatcg tcggtagcca ccacaacttc ttgaatacaa gtagtttggc
      901 gcacttgccg atacacccgc tcaatgagcg actgcccccc gatgtcggcc aatggtttgc
      961 ccggaaagcg cgtggaagca tatctggcag gaatgatgcc ggctattttc atgcgtacaa
     1021 attttactcg tgcaaatcgt agctccagga aatggtggcc actttttcca ccatgcgcgt
     1081 cacggagcag tatttttccn nnnnnnnnnn nnngcgcgct cggctttggc agcatctaag
     1141 gggccgtaca ggtcgtagtg tacatgtatg gttttgaata cttcgggaat ggcgccttct
     1201 tcgcgctcgc cggttacaga cactttgatg tcgcgcaatt cctggcgctt tttcttcata
     1261 atgaggatga catctatgga gctgcaagcc cccaaggctg agagtagcat ctgcatgggg
     1321 cgcatgcctt tgccggtgcc gccgatggcc ggggaagcgt cggtttcaac ggtattgccg
     1381 tcttcgttga cagcctgaag gtggtagtcg ccactgagac gacgcaattc gattttcata
     1441 aggtaatttt ccacaaagat agcgattaag cctttacttt gctttgtgaa ccagatcaca
     1501 ataggcctga ttttcctcgg atgcgcagcc gctgctggtt atgcgttgct ctgtttttgg
     1561 gttgttcgca actggcgcag actgcccgaa tggacggtgc cggaggcgtt tgttccgcac
     1621 acttcggtca gcgtggtcat tccggcccgc aatgaagccg ccggcattga agcctgtctg
     1681 cgctcggttt ttcagcaaaa ctaccccccg catttgctcg aaatcatcgt agtggacgat
     1741 cactccgaag acgatacagt tgccatagtg ggaagtctga atttgcccaa cctgcgcctg
     1801 ctccgtctag ccgattttgt gcagcccggc gctactacct ccttcaaaaa aaaggccatc
     1861 aaaacagcca tagctcatgc tcgcggcgaa ctgatcgtca ccaccgacgc cgactgcacc
     1921 gccccgcccg actggctgcc tgccatcgtc tcgctgtacg aacaacgccg cccgaaagcc
     1981 attctcgcat cggtgggact ggccccggca gccaacaaaa tggagcggtt tcaggcattg
     2041 gacatggccg ggctgatgct ttgttccggc gca
//
LOCUS       DAORLF010000627         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28916_length_2059_cov_1.794910, whole genome shotgun sequence.
ACCESSION   DAORLF010000627 DAORLF010000000
VERSION     DAORLF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28916_length_2059_cov_1.794910"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1314
                     /locus_tag="PK198_27020"
     CDS             259..1314
                     /locus_tag="PK198_27020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42483.1"
                     /translation="MKTDGTPEGTTVLLTDTVVGDDRFYFGHTMKVLDERVWMIIKGG
                     DNRWGLWRTNDDASAIELVVSFNPVLGDAQSPFLSTNGNHLYFFANDDFNTLQYYQSD
                     GTIAGTRPFLDANVSVRPTGIRNTHLFWRNKLYYTSNSPEYGPELFVSDGTAEGTYVL
                     ADILPGVHGSDPFQLGAAFTDSLLFFTAFTPQHGRELWQTDGTPEGTFMVQDICPGPC
                     SSSPDQLNIIGDGTLLFSAYHPGVGIEPWIYRFAAGPSTETEEIYSPSLERLLVFPNP
                     NAGERFYVSLPEMRGSHRIEILDLRGVVLASERMAGAGRRVHEMTLPGLSNGMYLLRL
                     LDAGRLRAVEKLMVMGR"
     gene            complement(1383..>2059)
                     /locus_tag="PK198_27025"
     CDS             complement(1383..>2059)
                     /locus_tag="PK198_27025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42484.1"
                     /translation="DVVEEMYLNRDGSGKYSITIDMSGLFEDEFMKSMIKSSLEQDTT
                     LKLGGANGIPEMDTIIYFKDMPAEQKGSNPEFWNRVHSKIVMSEEKGKFFTSINLDFA
                     STADITYLYENIGKIGESNSQLGGLAGEGGLLPTNVGYTFAKNMLTRKSPKSTEKAEG
                     GEDMEMMKMFLGSANFRTIYHLPGNVKKVTIPNAKTDGKTVTVEASMIDMMDGKANLD
                     GTIRFK"
BASE COUNT          451 a          494 c          555 g          559 t
ORIGIN      
        1 gcaagtctgg aactttgggc cagtaacggc caaccagggc aggagcgcaa ggtcacggat
       61 ttgatatccg gaccatatag ctacggggta tctgatggcc gattggtcta tctcggaaat
      121 ataaatcaag gactctaccg aaccgacggc actgcggccg gtaccatgga attaatacct
      181 gaagtaggtt ttccccttga ggctcttgta ttcaatgggc atacctatct gaggatgaca
      241 aataatcgta accgacgctt gaaaactgac ggcacccccg aaggtaccac tgtgttattg
      301 acagacacag tggttggtga tgaccgtttt tactttggcc ataccatgaa agtgcttgat
      361 gagcgtgtgt ggatgatcat aaaaggagga gacaaccgtt ggggactgtg gcgcaccaac
      421 gacgacgcat cggcgataga gttggtagtg tcgttcaatc cggtgctggg agatgcgcag
      481 tctccttttt tatctacaaa cggaaatcat ctgtattttt ttgcgaatga tgacttcaac
      541 acacttcaat attatcagtc agacggcacc atcgccggca caagaccttt tctggatgcg
      601 aatgtctccg tacgaccgac gggcatcagg aatacccatt tattctggcg caacaaactg
      661 tactatactt ccaactcacc tgaatacggc cctgagttgt ttgtcagtga tggtactgct
      721 gagggtacct atgtcttggc tgacatcttg cctggcgtac atggttccga tccctttcag
      781 cttggcgccg ctttcaccga ttcgctctta ttctttacgg ctttcacacc gcagcatggc
      841 cgggagcttt ggcaaaccga cggcacgccc gaaggtactt ttatggtaca agacatctgc
      901 cccggccctt gcagttcttc ccccgatcaa ctgaacatca taggcgacgg gacgctgctg
      961 ttcagcgcct atcatcccgg cgtaggcata gagccttgga tatatcgctt tgcagcaggg
     1021 ccttctaccg aaacggagga aatctacagc ccttcgctcg agcgtttgtt ggtgttcccc
     1081 aatcctaatg ccggcgagcg gttctatgtg tccttgccgg aaatgcgggg cagtcatcgc
     1141 atagaaatac tcgatttgag gggagtcgtg ctggcatcgg agcgaatggc gggcgctggg
     1201 cgtcgtgtac acgaaatgac gttgcccggt ttgagcaacg ggatgtatct gctgcggctg
     1261 ctggatgccg ggcgactgag ggcagtggaa aagttgatgg tgatggggcg gtgagtgtgt
     1321 ccttgtatta caagacaatg cgaggttgtt gaaatctcgc attgtcttga ttctcaataa
     1381 gattacttaa acctgatcgt tccgtccaaa ttggctttgc cgtccatcat gtctatcatg
     1441 gaagcctcta cggtgacggt tttgccgtct gttttggcgt tcggaatggt cacttttttc
     1501 acattgccgg gcaggtgata gatggtgcgg aagttggccg atccgagaaa catcttcatc
     1561 atctccatat cttcgcctcc ctctgctttt tcagttgatt tgggcgattt gcgggtgagc
     1621 atgtttttgg caaaagtgta gcccacgttg gtaggcaaca ggccgccttc gccggcaagg
     1681 ccgcccaatt gcgagttgct ttcgcctatt ttgccgatgt tttcgtacag ataggtaatg
     1741 tcggcggtgg aagcgaagtc caaattgatg gaagtgaaga acttgccttt ttcttcactc
     1801 atcacgattt tggagtggac gcggttccag aactccggat tactgccttt ttgctcagcg
     1861 ggcatgtctt tgaaatagat aatggtgtcc atttcgggaa tgccgttggc gccgccgagt
     1921 ttcagcgtgg tgtcttgttc cagcgaagat ttgatcatgc ttttcatgaa ctcatcctcg
     1981 aagaggccgc tcatgtcaat ggtgatggaa tatttgccgg aaccgtcgcg gttgaggtac
     2041 atttcttcaa ccacatcga
//
LOCUS       DAORLF010000628         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_28949_length_2057_cov_2.007992, whole genome shotgun sequence.
ACCESSION   DAORLF010000628 DAORLF010000000
VERSION     DAORLF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28949_length_2057_cov_2.007992"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK198_27030"
     CDS             <1..499
                     /locus_tag="PK198_27030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42485.1"
                     /translation="SINVYPIPWQPFIQANGNLLFSSTWFGNQWFLNGERIPGATDRQ
                     YLATTSGFYSVQVTSGGCVSPMSNLFNYFVVSVPEVSENDAIRLFPNPAQNEVFIHSI
                     WFDDTVCDLIIYNLAGAKMQALKCSFVSGIAQFEIKALPAGVYWVQIIGRGGEKRAIN
                     KLIKI"
     gene            610..>2057
                     /locus_tag="PK198_27035"
     CDS             610..>2057
                     /locus_tag="PK198_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF42486.1"
                     /translation="MRSLLLCCLLFVGFCALAQVQKAPDRQEGDGPYTQLIIRGVTLI
                     NGDGSPPRGPMDIVVEGNRITGIHAVGYPGVPVEEKGRPQLKSGGHELNAEGMYLLPG
                     FIDMHGHIGGRWQGADAEYVFKLWMGHGITTIRDPSAGNGLDWTLDQRGRSAKNEITA
                     PRIHAYTVFGMGSEEPISTPEQAREWVRNNAKKTADGIKFFGAAPQLMAAALDENKKL
                     GLRSACHHAQMDVARWNVLHSARAGLTSMEHWYGLPEALFADRTVQDYPLDYNYQNEQ
                     HRFEEAGKLWKQAAPPYSEHWNKVMSEMLALDFTIDPTFNIYEANRDLMRTRRAEWHD
                     EYTLPSLWRFFAPNRISHGSYWFNWGTEQETVWRENYRLWMTFINEYKNRGGRVTAGS
                     DSGFIYQLYGFAYIRELELLREAGFHPLEVIRSATMYGAQALGVEAKDLQTANFSVAA
                     QQDYDPVTGQARATFTYVVDNTLTISVRDLAKL"
BASE COUNT          476 a          610 c          521 g          450 t
ORIGIN      
        1 ttcaataaat gtatatccta ttccctggca gccatttatt caggctaacg gcaatctttt
       61 gttttcttcc acttggtttg gcaaccagtg gtttcttaat ggagaacgaa taccaggggc
      121 tacggatcgg caatatttgg ctactacgag cggattttat tccgtacagg taacctctgg
      181 gggctgcgtt tctcctatgt ctaatttatt caattatttt gtagtttctg tcccggaggt
      241 ttctgaaaat gacgccatcc ggttatttcc caaccccgct caaaatgaag tgttcattca
      301 ttctatatgg tttgatgata ctgtttgtga tctgattatt tataatcttg caggcgccaa
      361 aatgcaggcg ctcaagtgca gttttgtttc tggaattgca cagtttgaaa taaaggcttt
      421 accggcaggg gtttattggg tacaaataat tggacgagga ggagagaaac gggcaataaa
      481 caagctgatt aaaatataac tctgcgacgc tccttcccgc aggagtacac ccgtaaatcg
      541 tactgaaata gaataagagg aacagcgacc gccgcaaatc ccttatcttg ccccacaaaa
      601 aaaaacatca tgcgttcatt gctcctctgc tgccttcttt ttgtcggctt ttgcgccctt
      661 gcacaggtac aaaaagcgcc cgaccgccag gaaggcgacg gcccttacac tcagctcatc
      721 attcgcggcg tcaccctcat caacggcgac ggctcgcccc cgcgcggccc tatggacatc
      781 gtggtggaag gcaatcgcat caccggcata cacgcagtag gttatcccgg cgtaccggtg
      841 gaggaaaaag gccgccccca actcaaatcc ggcggccacg aactcaacgc cgaaggcatg
      901 tacctcctgc ccggcttcat tgacatgcac ggccacatcg gagggcgctg gcagggcgcc
      961 gatgcggaat acgtcttcaa actctggatg gggcacggca tcactaccat ccgcgaccca
     1021 tctgccggca acggattgga ctggacgctg gaccaacgcg gccgcagcgc caaaaatgaa
     1081 atcaccgcgc cccgcataca tgcttacacc gtgttcggca tgggcagcga agaacccatc
     1141 tccacgcccg aacaggcccg cgaatgggtg cgcaacaacg ctaaaaaaac cgccgacggc
     1201 atcaaatttt tcggcgccgc gccccaactc atggccgccg ctttggacga aaacaaaaaa
     1261 ctcggcctcc gttcggcctg ccaccatgcc caaatggacg tggcccgctg gaatgtactg
     1321 cactcggccc gcgccggtct cacttcgatg gaacactggt acggcctgcc cgaagcgctc
     1381 ttcgccgacc gcaccgtgca ggattatccg ctcgattaca attaccaaaa cgagcagcac
     1441 cgcttcgagg aggctggcaa actctggaaa caggccgcgc cgccatattc cgagcactgg
     1501 aacaaggtga tgtcggaaat gctggcattg gattttacca ttgatcccac cttcaatatt
     1561 tatgaggcca accgcgacct catgcgcacc cgccgcgccg agtggcacga cgaatacacc
     1621 ctgccctcgt tgtggcgttt ttttgcgccc aaccgcatct cgcacggctc ctattggttc
     1681 aattggggca ccgagcagga aaccgtctgg cgcgaaaact accgcctgtg gatgaccttt
     1741 atcaatgagt acaaaaaccg gggcggccgc gtgacggcgg gttccgactc tggttttatt
     1801 taccaacttt atggttttgc gtacattcgc gaattggaac tgctgcgcga ggccggtttc
     1861 cacccgctgg aggtcattcg ctcggccact atgtatggcg ctcaggcgct gggcgtggag
     1921 gccaaggacc tgcagaccgc caacttctcg gtggcggccc agcaggacta tgaccccgtc
     1981 accggccagg cccgcgcgac gttcacctac gtcgtcgaca acaccctgac catcagcgtg
     2041 cgcgacctgg ccaagct
//
LOCUS       DAORLF010000629         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_29204_length_2043_cov_1.591046, whole genome shotgun sequence.
ACCESSION   DAORLF010000629 DAORLF010000000
VERSION     DAORLF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29204_length_2043_cov_1.591046"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>272
                     /locus_tag="PK198_27040"
     CDS             <1..>272
                     /locus_tag="PK198_27040"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRF42487.1"
                     /translation="GVVISDRVNNNWTTRNIALQDGASGIIVRFSADHTFNVGDSLVI
                     NIGGVELSEFSGLLQLNNVPIANATRPGAGTLPTPRVATIAQVLANL"
     assembly_gap    273..384
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(666..1301)
                     /locus_tag="PK198_27045"
     CDS             complement(666..1301)
                     /locus_tag="PK198_27045"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRF42488.1"
                     /translation="MVKISEKRLLSIAEYLVAEDAAEARNEFQNGKTIEMAGGTLSHN
                     VVKGEIFTLINMAIKAAHIPHLVLNSDTKVRIESDNRFFYPDVTISDGTPVYYTTPEG
                     RTRRDIIINPLVIVEVLSEDTRYYDKGEKFDMYCSVPGFQEYILIEPEQVWVKSMFLQ
                     DPAEHLWKHEVLTDKSARLPIRSLGWEIPLADLYTALEKLPKEEEEKDVEQ"
     assembly_gap    1428..1451
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1452..>1823)
                     /locus_tag="PK198_27050"
     CDS             complement(<1452..>1823)
                     /locus_tag="PK198_27050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42489.1"
                     /translation="DGRSFRELVRIQGAGTVSEPQFYEAFHNLPAAGVNYYRLAQYDY
                     DGTRTFSDIISVKFEGPETLAFIAPNPVSGNEINLWYNSPNETQIELEIIALDGKTCA
                     TQRRQVLSGQQYIHLSVDNLNA"
     assembly_gap    1824..1837
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1838..>2043)
                     /locus_tag="PK198_27055"
     CDS             complement(<1838..>2043)
                     /locus_tag="PK198_27055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42490.1"
                     /translation="PPLINCGAPFDAARRYGAYIEAQDCSQLQVLPVELVDFHGKAEE
                     KGTRLWWETASEHNNDYFAVEHSA"
BASE COUNT          416 a          468 c          509 g          500 t
ORIGIN      
        1 ggcgtagtca tcagcgaccg agtaaacaat aactggacga cgcgcaacat cgcgctccaa
       61 gacggcgctt cgggtattat cgtgcgtttc agcgcagatc acaccttcaa tgtcggcgac
      121 tcgttggtca tcaacatcgg cggcgtggag ctttcggaat tcagcggttt attgcaactc
      181 aacaacgtac ccattgccaa tgctacccgg cccggcgccg gcactttgcc tacgcctcgt
      241 gtagccacca ttgcacaggt attggccaat ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnactgtt cacgcgcacg gcagcttctt ttgccggctc
      421 caatgtgccc acaggaacag taacggtaac cggcgtagta tcgcaattca cggatgcaca
      481 gttgaatatt cgcaatttga gcgatgtgaa ttgatcgcgc gtttgcgaga cctccagggt
      541 atcgtaaacg taacatcgcc ccataaaaaa acgcctgccg ggatgtgttt ccggcaggcg
      601 ttttttggtt ggcgagctta gtgaaggggt gacttgccat gtaaatcata ttgatagtca
      661 ccccttcact gttctacatc tttctcttcc tcctctttcg gcaacttctc caaggcagtg
      721 tacaaatccg ctaaaggaat ctcccatcct aaagagcgaa tggggagccg ggctgatttg
      781 tctgttaaaa cctcgtgttt ccagaggtgt tcggccgggt cttgtaaaaa catagacttg
      841 acccaaacct gctccggctc tatgaggatg tactcctgaa aacccggtac ggagcagtac
      901 atgtcgaatt tttcgccttt gtcgtaatag cgggtatcct ccgaaaggac ttctacaata
      961 actaagggat tgatgatgat gtcgcggcgg gtacgacctt cgggggtggt gtaataaacc
     1021 ggcgtgccgt cgctgatggt cacatcgggg tagaaaaagc gattgtccga ttcgatgcgg
     1081 actttcgtgt cgctgttgag taccagatgg ggaatatggg ctgcttttat agccatattg
     1141 attaaagtaa agatttcccc tttcaccaca ttatgagata aagttcctcc tgccatttcg
     1201 atggtttttc cgttttgaaa ttcattccgg gcctcggcgg catcctccgc tacgaggtat
     1261 tcggcgatgc tgagcagtcg tttttcactt attttgacca tagcggactg gttttggctg
     1321 caagataaga gattttgggc gagcgcacaa gggtgcgtta tgggcgcatt ttggaaaact
     1381 tcaatacctg cgtttcgttg ccgtgtcgga ttcggagcag gtacaggnnn nnnnnnnnnn
     1441 nnnnnnnnnn ngcgttcagg ttgtccacag acagatggat gtattgttgg ccgctcagta
     1501 cctgccggcg ttgcgttgcg caggttttac catccaaagc aatgatttcc agttctattt
     1561 gtgtttcgtt ggggctgttg taccaaaggt tgatctcgtt gccgctcacc ggattggggg
     1621 caatgaaggc caaagtttcc ggcccttcga atttcacgga aatgatgtcg gaaaaagtgc
     1681 gcgtgccgtc gtaatcgtat tgcgccaagc ggtagtagtt gacgccggct gcgggcaggt
     1741 tgtgaaatgc ttcataaaat tgcggttcgg agacagtgcc agccccctgt atgcggacca
     1801 actcgcggaa ggagcgcccg tcgnnnnnnn nnnnnnncgg cagaatgttc cactgcgaag
     1861 tagtcgttgt tgtgctcaga ggcagtctcc caccacaagc gcgttccttt ttcctctgct
     1921 tttccatgaa aatcaacgag ttctacgggc aatacctgca attgggagca atcctgcgcc
     1981 tctatgtaag ctccgtatct tcgggcagca tcgaagggtg cgccgcaatt gatcaagggt
     2041 ggg
//
LOCUS       DAORLF010000630         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_29306_length_2037_cov_1.465187, whole genome shotgun sequence.
ACCESSION   DAORLF010000630 DAORLF010000000
VERSION     DAORLF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29306_length_2037_cov_1.465187"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    272..407
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<408..803)
                     /locus_tag="PK198_27060"
     CDS             complement(<408..803)
                     /locus_tag="PK198_27060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42491.1"
                     /translation="MSSMQTGLDEYARTVEPDNEFYNRSGYRHGDMNNTLIKTHLGRT
                     ILVQHDVVTGRPYSRINMLAGTKAFHNGYPSRMSMSGKGHGWLSKEDYQTYRERFTHP
                     LWAKLKKEAETNGGHGGMDFVMLYRLIDCL"
     gene            complement(757..1650)
                     /locus_tag="PK198_27065"
     CDS             complement(757..1650)
                     /locus_tag="PK198_27065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF42492.1"
                     /translation="MSNPIKRRNFLRLGAAAASFFGFSRIASGRSEAEDQLARILESR
                     PAAGKPVIGLQVKPIPQVRVAVIGLGNRGEEHVRLVNAIGTQKARITAICDIRENVAQ
                     KTLEFLQKNGGQKPAVYSGKADAWKEMLRRDDIDLVLIATPWEDHVEMSVYAMQQGKH
                     VGLEVPAAYTVEDCWKLVDTAEATQRNCIMLENVCYGEEELWLLNMAANGVFGTLTYA
                     ECAYIHDLRELLYSKDYYYNQWRIRHNEARDGNLYPTHGLGPVSVMLPVASIHRKRDT
                     IRAPRVYEFHADRPRRVRPHR"
     gene            complement(1669..>2037)
                     /locus_tag="PK198_27070"
     CDS             complement(1669..>2037)
                     /locus_tag="PK198_27070"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF42493.1"
                     /translation="PAEQEGFVAEKDGQFIGLITFERSGDAVEITLLDSRERQQGIGS
                     RLVELALEYAKNCQCSRVWLVTTNDNIRAIRFYQKRGFDLVALHRNALEAARLLKPEI
                     PMTGQDGIPIRHELEFCITL"
BASE COUNT          347 a          502 c          586 g          466 t
ORIGIN      
        1 gagtactgca acgcagggtc gttccgcagg gcggcgcgca ggcggtgttt gaagtgtcgg
       61 cattgccgga aggcgtgtat tttgtgcgga tgcagggaga tgtgcgaagt tttcgctttg
      121 tgcgatagca ccggtgaggc gctaacttcc ggttagctca tcacttcgcc ggaaaggtga
      181 ggcgctaatc tccggttagc gcctcacttc ttctgaaaaa ccaagcccta tcaaggatgc
      241 accatcacgc cctgcttgcg cggctctttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag gcaatcaatg
      421 aggcggtaga gcatcacaaa gtccatgccg ccgtgcccgc cgttggtttc ggcttctttt
      481 ttcagtttgg cccacagcgg gtgcgtgaag cgctcgcggt aggtttggta gtcttctttg
      541 gagagccagc cgtgcccttt gccgctcata gacatgcggc tggggtagcc attgtggaag
      601 gctttggtac ccgccagcat gttgatgcgg ctgtacgggc ggcccgtcac cacatcgtgt
      661 tgcaccaaga tggtgcgtcc cagatgcgtt ttgatgaggg tattgttcat gtcgccgtgg
      721 cgatagccgc tgcgattgta gaactcgttg tcgggttcaa cggtgcgggc gtactcgtcg
      781 aggccggtct gcatggaact catagacacg aggtgctcga atcgtatccc tctttcggtg
      841 aattgacgcg accggcagca tgacgctcac cggcccgagg ccgtgcgtgg ggtacaggtt
      901 gccgtcgcgg gcttcgttgt gccgtatgcg ccactgattg tagtaataat ccttgctgta
      961 caacagttcg cggaggtcgt gtatgtaggc gcactcggcg taggtgagcg tgccgaatac
     1021 accgttggcg gccatgttga gcagccagag ttcctcttcg ccgtagcata cattttccag
     1081 catgatgcaa ttgcgctggg tagcttcggc ggtgtccacc aatttccagc aatcctctac
     1141 ggtataggcg gcgggtactt cgaggccgac gtgcttgccc tgctgcatgg catacaccga
     1201 catttccaca tggtcttccc agggggtagc aatgagtacg aggtcaatgt cgtcgcggcg
     1261 cagcatttct ttccaggcat cggctttgcc ggaatagacg gcaggttttt ggccgccgtt
     1321 tttttgcaaa aactccagcg tcttttgggc cacattttcc cgaatatcgc agatggcggt
     1381 gatgcgggct ttttgtgtac cgatggcatt gaccaagcgg acatgctctt caccgcggtt
     1441 gcccaaacca atgacggcca ccctcacttg cggaatgggc ttgacctgca atccgatgac
     1501 gggcttgccg gcagcggggc ggctttcgag gatgcgggcc aattggtctt cggcctcgct
     1561 gcgcccggag gctatgcggc tgaatccgaa aaaggaggcg gcggcagcgc cgaggcgcag
     1621 aaaattgcgg cgtttgatcg ggttggacat gatgcgatga tgaatatttt atagtgttat
     1681 acaaaattcc aattcgtgcc ggatgggaat gccgtcctgt ccggtcatgg gtatttccgg
     1741 tttcagcagg cgggcggcct ccaatgcgtt gcggtgcagc gccacgaggt cgaagccccg
     1801 tttttggtaa aacctgatgg cgcggatgtt gtcgttggtc gttaccaacc acactcggct
     1861 gcattggcag tttttggcat attccaaagc cagttctacc aagcgggagc caatgccctg
     1921 ttgcctttcg cggctgtcga gcagggtgat ttccacggcg tcgccggagc gctcaaaagt
     1981 aatcaatccg atgaattggc cgtctttttc agctacaaaa ccctcttgct cggcggg
//
LOCUS       DAORLF010000631         2029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_29447_length_2029_cov_2.036474, whole genome shotgun sequence.
ACCESSION   DAORLF010000631 DAORLF010000000
VERSION     DAORLF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29447_length_2029_cov_2.036474"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>889)
                     /locus_tag="PK198_27075"
     CDS             complement(<1..>889)
                     /locus_tag="PK198_27075"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HRF42494.1"
                     /translation="NTYGLRIGAQSWTADFGDVDNDGDMDCFITNHDLPSQLLLNDGS
                     GVFSDATTGSGINVQGLPIQGIFKDFDNDGFLDILVSGTRHYLYRNNGNGTFTDFGVA
                     AFGDTQMESFAVGDLNQDGKLDVYGGYANVYTSPSNIPDVIWLNTIANDNHYLTVRLE
                     GTTSNRNAVGARISIYGDWGIQIREVRSGESYGIMNSFFQHFGLGQQTSVDSLVVNWP
                     SGLREVFYNVPADQSIQIKEGECISPAASVALSGPTVFCPGESASLSITTASSVITWS
                     NGATGAELTVTEGGWYSAVV"
     assembly_gap    890..977
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<978..1623)
                     /locus_tag="PK198_27080"
     CDS             complement(<978..1623)
                     /locus_tag="PK198_27080"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF42495.1"
                     /translation="MKSNFYFLLAFLALAGVSLQAQISFTNANAKLGYGNHFSGVAIG
                     IADMNADGYDDIVHLRNGRILAIELQKPDGSGLQTITVGQISNNSQWSMCIADADNNG
                     FNDVLAGGSYDGVKLVRAASDGQSYTWANLQGTGLFLQGSNFADINNDGWLDVFACHD
                     DAMSRIWGNDGTGQFLIESWIDMTTVPPSDNSGNYGSVWTDFDNDGDLDLYIAKC"
     gene            complement(1695..>2029)
                     /locus_tag="PK198_27085"
     CDS             complement(1695..>2029)
                     /locus_tag="PK198_27085"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRF42496.1"
                     /translation="GWHTQNLFRSIYFAAQCGAAELSTGLLALTHLHGKGKVSMLITR
                     VEADFVKKADSLVTFTCTDGDAMRAAIEEAVQTGTAVAFTARSVGVQATGEIVAQLHF
                     TWSFKSKP"
BASE COUNT          459 a          499 c          546 g          437 t
ORIGIN      
        1 tactaccgcg ctgtaccaac cgccctcggt caccgtcagc tctgcgcccg tagcgccatt
       61 cgaccaggta atgaccgatg aagccgtcgt tatcgaaagg cttgcacttt cacccgggca
      121 aaataccgtt gggccggaca gcgccacaga tgccgcagga gaaatgcact cgccttcttt
      181 gatttgaatg gactgatcgg cgggaacatt gtaaaacacc tcgcggaggc cggagggcca
      241 gttgacgact aacgaatcta cgcttgtctg ttgtcccagt ccgaagtgct ggaaaaagct
      301 gttcataatg ccgtagctct cgccgctgcg cacttcgcgt atttggatgc cccagtcgcc
      361 gtaaatggaa atgcgcgcac ctacagcatt gcggttggaa gtagtgcctt cgaggcgaac
      421 ggtgaggtag tgattgtcat tggcaatggt attcagccag atcacgtcgg gaatgttgct
      481 gggcgaggtg taaacattgg cgtagcctcc gtaaacgtcc aatttaccgt cctgattgag
      541 gtcgcccact gcaaaagact ccatttgtgt atctccgaaa gcggccacgc caaaatcagt
      601 aaaggtgccg ttcccgttgt ttctgtacag ataatggcgc gtgccggaaa cgaggatatc
      661 gagaaaaccg tcgttatcga aatctttaaa aataccctga atgggcaatc cctgcacgtt
      721 gatcccggac cctgtggtcg catccgaaaa cacccccgaa ccatcgttga gcagcagttg
      781 gctgggcagg tcgtggttgg tgatgaagca atccatatct ccgtcattat caacatctcc
      841 gaagtcggct gtccaggatt gcgcgccgat ccgcagacca taggtattgn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnggc acttggcgat gtagaggtcg aggtcgccgt cgttgtcgaa
     1021 gtcggtccac acgctgccat aattgccgga gttgtcggaa ggcggcacgg tggtcatgtc
     1081 tatccagctt tcgatgagaa attgcccggt accgtcgttg ccccagatgc ggctcatggc
     1141 gtcgtcgtgg caggcaaaga catcgagcca gccgtcgttg ttgatgtcgg cgaagttgga
     1201 gccttgcaga aaaagacctg ttccctgcaa attggcccag gtatagcttt gtccatcgga
     1261 ggcagcacgc accaatttga caccgtcgta ggaaccaccg gcaagcacgt cattgaagcc
     1321 gttgttgtcg gcatcggcaa tgcacatgct ccattggctg ttgttggaaa tctggcccac
     1381 cgtaattgtt tgcaggccgg agccatcggg tttttgcagt tctatggcaa ggattctgcc
     1441 gttgcgcagg tgtacgatgt cgtcgtaacc atcagcgttc atgtcggcga tgcctatagc
     1501 cacgccgctg aaatgattgc cgtagccgag tttggcgttg gcattggtaa aggaaatttg
     1561 ggcttgcagc gaaacgccgg ccaaggccag aaaggccaga agaaagtaga agttgctttt
     1621 catagagatg acaaggacgt tgaaacaggt gatgaaactt aaaatttttt cggaggcgaa
     1681 aggtaaaaaa caaatcaagg cttggactta aaagaccatg taaaatgcag ttgcgcgaca
     1741 atttcaccgg tggcctgcac ccccacagaa cgggcggtaa aagcaactgc cgtgcccgtt
     1801 tgaacggctt cctcaatggc agcgcgcatc gcatcgccgt cggtacaggt gaaagtgacc
     1861 aaggaatcgg ctttttttac aaaatcggct tctacccgcg tgatgagcat ggacaccttt
     1921 cccttgccgt gcaaatgtgt aagcgccaac aggccggtgg acagctcggc agcaccgcat
     1981 tgcgccgcga aatagatgga gcggaaaagg ttttgtgtgt gccacccgt
//
LOCUS       DAORLF010000632         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_29645_length_2019_cov_1.999491, whole genome shotgun sequence.
ACCESSION   DAORLF010000632 DAORLF010000000
VERSION     DAORLF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29645_length_2019_cov_1.999491"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>368
                     /locus_tag="PK198_27090"
     CDS             18..>368
                     /locus_tag="PK198_27090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapM"
                     /protein_id="HRF42497.1"
                     /translation="MVSAGPIPPNPAELLLQDRTGEMFATLRQQFDYIIVDTPPIGLV
                     SDALLLNPHITGALYLVRQGVSKKGSLQLVDDLYIHKKLQNIAIVINGVGAKGGYGYG
                     YGYGYGYGYGYGYGY"
     assembly_gap    369..423
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <424..1405
                     /locus_tag="PK198_27095"
     CDS             <424..1405
                     /locus_tag="PK198_27095"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF42498.1"
                     /translation="MKKILYRLANLAYLLLKTRNPLHAFRFYHFKTEPLFYVSWQINS
                     KGLFFPDVPLLLPHSFCRRCGYDLNELCRLARNPYLRAIEQTDEVPVLWMQYRGEDIR
                     FQCRSIDNLMVVNELFTDKIYEFEMGCPLVCIDIGMNAGLSTLLLAARADVQRIYAYE
                     PFRSTYDLAMSNIAINKTLQAKINPIHAGWSDKEGMFSFHNYEEGSLGASTSFTADLH
                     TYGQTAGTTEVQLLDASSELRRIMQLHPDQSLFVKLDCEGSEYEILEKLDHDGLLPYI
                     HAFLMEWHHKGAAPIEEILKKNGFSFFSTGRPDVAQIGFVYAVNTSNHGV"
     gene            1395..>2019
                     /locus_tag="PK198_27100"
     CDS             1395..>2019
                     /locus_tag="PK198_27100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF42499.1"
                     /translation="MEFKSLFQKLLRGIGIDIRLVSTIQKKEALHHLWLQQSGIHCIF
                     DVGANQGQFATQIRQYFPHTDIYSFEPIDTCFYSLQQRFQSDQHFKAFQYAVGDENTE
                     TTFHLNESSPSSSLLKMQPAHYQNYPGTQKTVDQKVAVRTLDSLFPDWPADKKILLKI
                     DVQGYEYQVLKGAAQLLQQVEVILAEVMFEELYESQSTFDQLNELLRA"
BASE COUNT          513 a          513 c          438 g          500 t
ORIGIN      
        1 cgtttaaaaa tttatatgtg gtttctgccg gccccattcc gcccaatccg gctgaattgt
       61 tgcttcagga tcgaaccggc gaaatgtttg caacactgcg ccaacaattc gactatatca
      121 ttgtggacac gccgcccatc ggcttggtat ccgacgcgct gttgctcaac ccgcacatta
      181 ccggagcgct gtatttggtg cgtcagggcg tgagtaaaaa gggttctctc caactcgtgg
      241 acgatcttta catccataaa aagctccaaa acattgccat cgtaatcaat ggcgtgggcg
      301 ccaaaggcgg ctacggctat ggctacggct atgggtacgg ctatgggtat gggtatggct
      361 acggatacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnngatgaaa aaaatactgt acaggctggc caaccttgcc tatttgttgc tgaaaacgcg
      481 gaatccgttg catgcttttc ggttttatca tttcaaaaca gagccgcttt tttacgtttc
      541 ctggcaaatc aattccaaag gacttttttt ccccgatgtt ccgcttttgc ttccccacag
      601 cttttgccga cgttgtggct acgacctgaa cgaactgtgc agattggcca gaaacccgta
      661 cctccgagcc atcgaacaaa cagatgaagt gccggtatta tggatgcagt atcgcggaga
      721 agatattcgg tttcagtgcc gatcaataga caacctcatg gtagtcaacg agttgtttac
      781 cgacaaaatt tatgagtttg aaatgggctg cccgctggtt tgtattgata ttggtatgaa
      841 tgccggcctg tccacgctcc tgttggccgc ccgcgccgat gtacagcgta tttatgccta
      901 cgaacccttc cgcagcactt atgatctggc catgtccaac atagccatca acaaaacatt
      961 gcaggcaaaa atcaacccga tacacgccgg ctggagcgac aaagagggca tgttttcttt
     1021 ccataattat gaagaaggca gcctcggcgc ttctaccagt tttacagccg atctgcatac
     1081 ctacggccaa accgccggca ctaccgaggt acaactttta gacgcatcct ccgaactccg
     1141 ccgaatcatg caattgcatc ccgatcagtc gctttttgta aagctcgact gtgaaggttc
     1201 ggaatacgaa atattggaaa aattagatca cgacggcctg ctcccctata tccacgcttt
     1261 tttgatggaa tggcaccaca aaggcgcagc acccattgag gaaattctga aaaaaaacgg
     1321 attttctttt ttctccaccg gcaggcccga cgttgctcaa atcgggttcg tttacgccgt
     1381 caataccagc aatcatggag tttaaaagcc tgtttcaaaa actgttgcgc ggcataggca
     1441 tagacattcg cctcgtttct accatccaaa aaaaagaagc gctgcaccac ttatggcttc
     1501 aacagtcggg tatccattgc attttcgacg tgggcgccaa tcaggggcaa tttgcaacgc
     1561 agatccgaca gtattttccg cacaccgata tttattcatt tgaacccata gacacctgtt
     1621 tttactcgct tcaacaacgt tttcagtccg atcagcactt caaggctttt caatatgcgg
     1681 tgggtgatga aaatacagaa acgacttttc acctcaacga atcttcgccc tcgtcttccc
     1741 tgctgaaaat gcagccggct cattaccaga attatcccgg cacccaaaaa acagtggacc
     1801 aaaaggtagc tgtccgcact ttggacagcc tgtttcccga ctggcccgcc gacaaaaaaa
     1861 ttctgctgaa aatagacgtg cagggctatg aatatcaagt attaaaaggc gctgcgcaat
     1921 tgctccagca ggtggaagtc attctggcag aggtcatgtt tgaagaattg tacgaaagcc
     1981 aatccacctt cgatcagctc aacgaactgc ttcgcgcac
//
LOCUS       DAORLF010000633         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_29646_length_2019_cov_1.962831, whole genome shotgun sequence.
ACCESSION   DAORLF010000633 DAORLF010000000
VERSION     DAORLF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29646_length_2019_cov_1.962831"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK198_27105"
     CDS             complement(<1..388)
                     /locus_tag="PK198_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF42500.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEEEVDLAKRIK
                     QGDQAALEKLTKANLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPL"
     gene            complement(389..>648)
                     /locus_tag="PK198_27110"
     CDS             complement(389..>648)
                     /locus_tag="PK198_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42501.1"
                     /translation="FVNQALSVLFTPSEKYSLDMCRKKPYFCSPKAKSTLRANLAAGS
                     IVSTKRVKKVCDAARFLPSQRASQSALYQENKPLLKHKIGH"
     assembly_gap    649..672
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            771..1151
                     /locus_tag="PK198_27115"
     CDS             771..1151
                     /locus_tag="PK198_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRF42502.1"
                     /translation="MERVHFTLEYLFKASPAILYQFLTDPPCLIRWFCDEVDINVDVY
                     TFKWGKSEESAYVVDDFEEELFRLHWESADADEEHLEFKIFTSDVTDETILHISDYCD
                     RGEEEDQQRYWDALMTRLRAVCGG"
     assembly_gap    1290..1314
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1315..1860)
                     /locus_tag="PK198_27120"
     CDS             complement(<1315..1860)
                     /locus_tag="PK198_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRF42503.1"
                     /translation="MKVIIFGLGNYGVSLAGSLMEAGNEVIGVDKDPLRVDAVKDDVT
                     YAVILDSTNEAAVRTLPFKDVDAAIVAIGENEGAAILTAAILKKMGVRRIISRSISPL
                     HQMVLEAMGIEEIVHPEQESADRLSKMLHLKSVVNNFEVDDRYSVTEIQLDDRFVGKT
                     IQEVGFRAQYQLNIITIIREQT"
BASE COUNT          449 a          480 c          517 g          524 t
ORIGIN      
        1 tgagcggcag gcgcacgatg cggctttgtt cggccaatgc ctggaggatg gactggcgaa
       61 tccaccacac ggcgtaggaa atgaatttga aaccgcgggt ttcgtcaaat cgctgggcgg
      121 ctttgatgag gccgagattg ccttcattga taaggtcgct gagcgagaga ccctgatttt
      181 gatattgttt ggctacagaa acgacgaaac ggaggttggc tttggtaagt ttttccagtg
      241 ctgcctgatc tccctgtttg atgcgtttgg ctaagtccac ctcctcttcc ggggtcagca
      301 ggtccacttt tccgatctcc tgcaaatact tttcgagcga ctggctctcc cggttggtaa
      361 tagatttggt gatctttagt tgtctcattc aatgaccgat tttatgtttt aataatggct
      421 tattctcttg atagagagca ctctgcgatg cccgttgcga cggtaaaaaa cgagctgcgt
      481 cacatacttt tttcactcgc tttgtggaaa caatacttcc cgccgcaagg ttggcgcgga
      541 gggtgctttt cgctttcgga ctgcaaaagt aaggcttttt tcggcacatg tcaagactgt
      601 atttttctga tggtgtaaaa agcacgctaa gcgcttggtt tacaaatann nnnnnnnnnn
      661 nnnnnnnnnn nnggaaaagt ttacaggacg ttggtgaaat catttttttc ttttttattg
      721 cggcctcaaa gtagcgccca agctgtatgg tcgcgcatta tcaagaagtt atggaacgag
      781 tacattttac attggagtat ctgttcaagg catcgccggc cattctctat caatttctca
      841 ccgacccgcc ctgcctcatc cgttggttct gcgacgaagt ggacatcaac gtggacgtct
      901 ataccttcaa atggggtaaa agcgaggaga gcgcttatgt ggtggacgac tttgaggaag
      961 agttgtttcg cctgcattgg gagagcgcag atgcggatga ggagcatctg gagttcaaaa
     1021 ttttcacctc ggacgtaacc gacgaaacca tcctgcacat cagcgattac tgcgaccgcg
     1081 gcgaggagga agatcagcaa cgctattggg atgccctcat gacccgcctg cgcgccgtgt
     1141 gcggcggctg aaaagccggc aacttattcg caaaaggcgc tgatggacgc attggcgccg
     1201 aacactacca aaatatcgcc tttttgcagc accgtgtctg ccgtaaccac cccgaccgac
     1261 tcccggatga cccgcatacc gcctaatacn nnnnnnnnnn nnnnnnnnnn nnnnggtttg
     1321 ttcccggatg atggtgatga tgttgagttg atactgcgct ctgaacccta cttcctgaat
     1381 cgtcttgccc acaaaccggt catccaattg tatctccgtg acggagtagc ggtcgtccac
     1441 ctcaaaatta ttgaccaccg atttgaggtg cagcattttg ctcaggcggt cggcgctttc
     1501 ctgctccggg tgtacgattt cctctatgcc catcgcctcc aataccattt gatgcagcgg
     1561 ggagatggaa cggctgatga tgcgccgaac gcccattttt ttcagaatgg cggcagtgag
     1621 aatggccgct ccttcatttt cgccgatggc cacaatggcg gcgtccacgt ctttgaaagg
     1681 cagggtgcgc acggcggctt cattggtaga gtctaatata acggcataag tgacatcgtc
     1741 tttgacggca tccacccgca gcgggtcctt gtccactccg atgacctcgt tgccggcttc
     1801 catcaggctg cctgccaatg aaacgccgta gttgccgagg ccgaagatga ttactttcat
     1861 ggtgagcgtg attagaaaga tgaataccgg gatattcctc tcccccggat agagtgttgc
     1921 aagttatgat ttgtacagaa agtacgggta aaaaatttac ggacgcctta gtagtggggc
     1981 aaaaataaat gctcattttc tttaggccaa aggattaga
//
LOCUS       DAORLF010000634         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30057_length_1995_cov_2.376804, whole genome shotgun sequence.
ACCESSION   DAORLF010000634 DAORLF010000000
VERSION     DAORLF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30057_length_1995_cov_2.376804"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1042)
                     /locus_tag="PK198_27125"
     CDS             complement(53..1042)
                     /locus_tag="PK198_27125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF42504.1"
                     /translation="MKIAIVVFLLLGLWGCSAPARLDAPQSATATPKEITDCHVHVMS
                     PAMIAMWKSMGIPFGKPDAAYSNIDSIISRLGTHRMKLVSMAYVYSSGEFSTGQGNIR
                     EMVRAENDYLALQKSKYPGSIEAYYGIDPMADFAMEEFIRCNDSLKLDGLKLHFNASQ
                     VYLTERKYQEKVKQVLEYAAAKKVPVLLHFDNSHRRFGLPDLEILSDSILAFIPKIHL
                     QIAHLGTSGGFNSRTKAFIDAYLELIRDPAHPLHRHELVFDISAVALDKDSDGVPHLD
                     EAAFAELAAYLRKIGMDKLVFGTDYPLYSSREYLDILRKKVKLSEEEITALLR"
     gene            complement(1039..1317)
                     /locus_tag="PK198_27130"
     CDS             complement(1039..1317)
                     /locus_tag="PK198_27130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42505.1"
                     /translation="MENESNQLIFNKNTMWINFSTVWITECGKKLQAAVIQVGTGENS
                     FQPTSNRKPWRSGEGSHENGKAISKFLHFPYFCNGPKISPVFSKKLPI"
     gene            1402..>1995
                     /locus_tag="PK198_27135"
     CDS             1402..>1995
                     /locus_tag="PK198_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRF42506.1"
                     /translation="MSIEIEPILKENPNRFVLFPIEHNDIWAFYKKSEANFWTAEEID
                     LSADLVDWNDKLNDDERHFVKHVLAFFAASDGIVNENLAINFVNEVQYTEAKFFYGFQ
                     IMMENIHSETYSLLIDTYIKDQREKDYLLRAIEHVDCVKKKADWALRWIGNGNFAERL
                     IAFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELIS"
BASE COUNT          503 a          500 c          466 g          526 t
ORIGIN      
        1 ttacttatct aatcctttgg cgaaaatgat cactttattt ttttatcgcc ccttagcgca
       61 acagcgccgt tatttcctcc tcgctgagtt tcactttttt tctcaggatg tcgagatact
      121 cccggctgct gtacaaggga taatcggtgc cgaacaccaa tttgtccatg cctattttgc
      181 ggagatatgc ggccagttcg gcaaatgcag cttcgtccaa gtggggcacg ccgtcgctgt
      241 ctttatccaa ggcaacggcc gaaatatcga agaccaactc gtggcggtgc agcgggtgcg
      301 cggggtcgcg gatgagttcc aaataggcgt caatgaaagc cttagttcgg ctgttgaatc
      361 cgccggatgt acccagatgt gcaatctgaa gatgaatttt gggaataaaa gccaaaattg
      421 aatccgataa aatttccaga tcaggcagtc cgaaacggcg gtgcgaattg tcgaaatgca
      481 acaaaaccgg caccttcttc gctgcagcat attccaaaac ctgttttact ttttcctgat
      541 atttccgctc cgtcagatac acctgggagg cattgaaatg cagcttcaac ccgtctaatt
      601 ttaagctgtc gttgcatctg atgaattcct ccattgcaaa atccgccatc gggtcaatgc
      661 cgtaatacgc ctcgatggaa ccggggtatt tagacttttg caaggccaaa taatcatttt
      721 cggcccgcac catttcgcgg atattgccct gccccgtgct gaactcgccg gagctataca
      781 catacgccat cgagacgagc ttcatcctgt gcgttcccag gcggctgatg atgctgtcta
      841 tgttggaata cgcggcgtcg ggtttgccga aagggatgcc catagacttc cacatagcta
      901 tcatggccgg actcatgaca tgaacgtggc agtcggtgat ctctttggga gtagctgttg
      961 cgctttgggg agcatcgagt cgggctggag ccgagcagcc ccacagtccg aggaggagga
     1021 atacaacaat ggcaattttc atatcggcag tttttttgaa aaaacagggc ttattttcgg
     1081 cccgttacaa aagtacggaa aatgcaggaa ttttgagatc gccttaccgt tttcatgcga
     1141 cccctccccg ctgcgccaag gcttgcggtt agatgtaggc tgaaaactat tttcgcctgt
     1201 tcctacctgt atcacagccg cttgcagctt ttttccacat tcagttatcc acactgtgga
     1261 aaagttaatc cacattgtat ttttattgaa aatcagttga ttagattcgt tttccacaat
     1321 gtgtggaaaa ttttccattg ccaaacgcgc catccgctgt attttagcgt tccgaaatca
     1381 aagagaaaac gcaaaagcat tatgagcatt gagatcgaac ctatattgaa ggaaaatccc
     1441 aaccgttttg tactcttccc cattgagcac aacgacatct gggcatttta caaaaagtcg
     1501 gaagccaatt tttggacggc agaggaaata gacttgtcgg cagacctcgt ggactggaac
     1561 gacaaactca acgacgacga acgccacttc gtcaagcatg tactggcctt ctttgccgcg
     1621 agcgacggca tcgtcaacga aaacttagcc atcaacttcg tcaacgaagt gcaatacacc
     1681 gaggccaagt ttttttacgg gtttcaaatt atgatggaga acattcattc cgaaacgtac
     1741 tccctgctta ttgatactta catcaaggac caaagagaaa aagactacct gctgcgcgcc
     1801 atcgaacacg tggactgcgt gaagaaaaaa gccgactggg cgctccgttg gatcggaaac
     1861 ggcaattttg ccgagcgtct catcgctttt gcggctgtgg agggtatctt tttctcgggt
     1921 tctttttgct ccatcttctg gctcaaaaaa cgcggcctca tgccgggcct caccttctcg
     1981 aacgaactca tctcg
//
LOCUS       DAORLF010000635         1985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30236_length_1985_cov_2.063731, whole genome shotgun sequence.
ACCESSION   DAORLF010000635 DAORLF010000000
VERSION     DAORLF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30236_length_1985_cov_2.063731"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1347)
                     /gene="lpxB"
                     /locus_tag="PK198_27140"
     CDS             complement(208..1347)
                     /gene="lpxB"
                     /locus_tag="PK198_27140"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRF42507.1"
                     /translation="MRYYLIAGEASGDLHGAHLIRHLRQEDPEADIRAWGGDLMSEAG
                     AEVVKHYRDLAFMGFWEVLKNLRTIMANFRFCKADILAWQPDVLILIDYPGFNLRMAK
                     WAKSMGIRVFYYISPQLWAWHSSRVHGIKATVERMFVILPFEEEFYQKYDYKVDFVGH
                     PLLDVVDEFVPPADFRAANGLDERPIVALLPGSRRQEIARMLEVMLQTAARFPDYQFV
                     IAGAPSIPRSFYDDIIRSSGVSGVHWTGNQTYALLSQARAALVTSGTATLETALFGVP
                     QTVCYRGSSISFFIARRLVNVKYISLVNLIADRPLVRELIQHQLHPDSLSEQLRLLLD
                     DDHTRQLREGYRELRLRLGERGAAQKAARGMVRALGWKNELLENP"
     gene            complement(1457..>1670)
                     /locus_tag="PK198_27145"
     CDS             complement(1457..>1670)
                     /locus_tag="PK198_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42508.1"
                     /translation="ANRMTGIVSRDGLSPHFRERTLSVVAIGLNAFIFNYYARRRFFV
                     NTMRGMVIATVICVGVWMALFWKNLF"
     assembly_gap    1671..1680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1780..>1985)
                     /locus_tag="PK198_27150"
     CDS             complement(1780..>1985)
                     /locus_tag="PK198_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42509.1"
                     /translation="KDPRGQKYYWLTGRFENDDLLPDTDLWALENGYVSVVASMHDLT
                     NYRAMDSVRPLEQLSTTENAASI"
BASE COUNT          443 a          527 c          592 g          413 t
ORIGIN      
        1 agaaggatgc tgtttttttt catgtcgctt tttgttttcg gtacaatgtt aggcgcaagc
       61 ctgaatctta ccaaaaaata ggcactcagc ggggatatgt gccgcctcaa ccgggcaggg
      121 ggtctgctgg agggttttgt accttatacc aaacgacata aaaaatgaag caaccctgtg
      181 tggacaatat cagaaaagga aatgaaatta agggttttct aatagctcat ttttccaccc
      241 caacgccctc accatccccc ttgccgcctt ctgcgccgcc ccgcgttcgc ccagccgcag
      301 gcgcagctcc cggtacccct cgcggagttg gcgggtgtga tcgtcgtcca agaggaggcg
      361 gagttgttcg ctgaggctgt cggggtggag ttggtgttga atgagttcgc gcaccaaggg
      421 gcggtctgcg atgaggttga ccaatgaaat gtatttgaca ttgaccaatc ggcgggcgat
      481 gaaaaacgag atgctgctgc cgcgatagca caccgtttgc ggcacgccga acagcgccgt
      541 ttcgagggta gccgtacccg atgtgaccaa tgcagcgcgg gcctgactga gcagggcgta
      601 ggtttggttg cccgtccaat gcacgcccga tacgccggaa gaccggatga tgtcgtcgta
      661 aaaactgcgg ggaatggagg gcgctccggc tataacaaac tgataatcag gaaaacgggc
      721 ggcggtttgg agcatcacct ccaacatgcg ggcaatttcc tgccgccggc tgccgggcag
      781 gagggccaca atgggccgct cgtctaagcc attggccgcg cgaaaatcgg caggcggcac
      841 aaactcgtcc accacatcga gcagcgggtg gcccacgaag tccaccttgt aatcgtattt
      901 ttggtaaaac tcctcctcga agggcaggat gacgaacatg cgctctaccg tagccttgat
      961 gccgtgtacc cggctgctgt gccaggccca caactgcggc gagatgtaat aaaacactct
     1021 gatgcccatg cttttagccc acttggccat gcggagattg aaacccggat agtctatgag
     1081 aatgagtaca tcgggctgcc aggccaggat gtcggctttg caaaaccgga aattggccat
     1141 gatggtgcgc aggtttttca gcacctccca aaatcccata aaagccagat cgcggtagtg
     1201 cttgaccacc tcggcgccgg cttcgctcat caggtcgccg ccccaggcgc ggatgtcggc
     1261 ttcggggtct tcctgacgca ggtggcgaat gagatgtgct ccgtgcaggt cgcccgaagc
     1321 ctcgccggct atgaggtagt atcgcataaa gggagaattt tccttcacat cagcgagatt
     1381 acaccttccg ccttccggtg gttgcaacag ggcgacagat gtcacctcgc tactgtgtca
     1441 ttccaaacag ttgctctcag aagaggtttt tccaaaaaag cgccatccag acgcctacgc
     1501 agatgacggt ggcgatgacc atgccgcgca tcgtattgac gaagaatcgg cggcgggcat
     1561 agtaattgaa aatgaacgca ttgaggccaa tggctaccac cgaaagcgtg cgctcgcgga
     1621 aatgcggaga cagtccgtcg cggctcacga tgccggtcat gcggttggcg nnnnnnnnnn
     1681 actgttcata aatggtgagc agcagcgcat aagcgacgaa gggcaccaca aggcccagca
     1741 gcaggcccag ccagagggca tttttttgag gcatgatgat catatcgagg ctgcgttttc
     1801 ggtggtggag agttgttcca gcgggcgcac gctgtccatg gcgcggtagt tggtgagatc
     1861 gtgcatggag gccaccaccg aaacgtagcc gttttcgagc gcccaaaggt cggtatcggg
     1921 caggaggtcg tcgttttcaa aacggccggt gagccaatag tacttttggc cgcgcgggtc
     1981 tttgg
//
LOCUS       DAORLF010000636         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30528_length_1970_cov_1.876240, whole genome shotgun sequence.
ACCESSION   DAORLF010000636 DAORLF010000000
VERSION     DAORLF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30528_length_1970_cov_1.876240"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1162
                     /locus_tag="PK198_27155"
     CDS             <1..>1162
                     /locus_tag="PK198_27155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42510.1"
                     /translation="LPSQAGANGLLNTGRGVVLKYDAFGVLKWYLEFSGQNYGIRDIE
                     TDSAGNCYVPIGHGNAYIIGSTDGRRRIVQGTKGSSIVKYDPNGRVIWVAEVNACSWE
                     GPSILDVELDDQGYLYVFGTNGECIEFRSSDGSTGLVLNGAPSGWLAKYTPDGEVLWA
                     KALQDAAGNAYIYGHSLRLDATGNIFICGETSAAVAKFNPVGEYLWSVNAIGEEIMAA
                     RNLDVDEWGNVYVAGMFRGRVAFAGLPPIDLGLPQHGSDYDLFVLKVNSDGQPLWLKS
                     GMAADRYSENTAIRCFEDELYVYGSFGLGQFEYDDLVVEGRSQQNAILLKMNAADGSY
                     LAHRLFDVPNPDVGRSSFYACYGRTLAIDTAGRLHLLGNIGYEADFENDTLHS"
     assembly_gap    1163..1202
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1203..1510
                     /locus_tag="PK198_27160"
     CDS             <1203..1510
                     /locus_tag="PK198_27160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF42511.1"
                     /translation="GLSTVNAVSDHFTPLTWTAYLAPNPASETVRLHFHSQQAETLTV
                     TISDILGKRLALFQKMNIQPGEQTLELPIDTGAPAGIYWARLQADDGAVLMLKLVKK"
     assembly_gap    1583..1594
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1633..>1970
                     /locus_tag="PK198_27165"
     CDS             1633..>1970
                     /locus_tag="PK198_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRF42512.1"
                     /translation="MSDIGKTVGVVGAGSFGSAIGNLLAHNVDVLLYSRQPELVEAIN
                     TTHQHFGVALSHRVRATSHIEEMADQCRLIIPVVPSMRFREMMQRLGPHLRPYHVLIH
                     ATKGFDLHDTP"
BASE COUNT          452 a          451 c          512 g          503 t
ORIGIN      
        1 gttgccttct caggcaggcg ccaatggttt attgaataca ggtcggggcg ttgtcctgaa
       61 atacgatgca tttggcgtgc tgaaatggta tttggaattt tccggccaaa attacggcat
      121 ccgcgatatt gaaaccgact cagccggcaa ttgctatgtg ccgatcgggc atggcaacgc
      181 atacattatc ggctctaccg atggtcgccg ccgcatagtg cagggtacta aaggttcgtc
      241 cattgtcaaa tacgacccga acggacgcgt gatttgggta gcagaagtga atgcctgttc
      301 atgggaaggt ccttctattc tggatgtgga actggatgat cagggctatt tgtatgtgtt
      361 tggcacgaat ggagaatgta ttgaatttcg atcttcagac ggatccaccg gtcttgtatt
      421 aaacggcgct ccttctggtt ggctggcaaa atatacgccc gacggcgagg tcttatgggc
      481 aaaagccttg caagacgctg cgggcaatgc ttatatttat ggccacagcc tccggcttga
      541 tgcgacaggc aatattttta tttgcggtga aacttcggcg gctgtggcca aattcaatcc
      601 tgtcggcgaa tacctctgga gtgtgaacgc catcggagag gaaatcatgg cggcccgcaa
      661 tctggatgtg gacgagtggg ggaatgttta tgtggcgggt atgtttagag ggagagtagc
      721 atttgcaggc ttacccccaa ttgacttggg attgccgcaa cacggcagcg attacgattt
      781 gtttgtactg aaagtcaatt ccgatggtca acctttatgg ctcaaaagcg gcatggccgc
      841 agatagatat tccgaaaata cagccatccg atgttttgag gatgaactat atgtttatgg
      901 cagttttggg ctggggcaat ttgagtatga cgaccttgtg gtggagggcc ggtcgcaaca
      961 aaatgctatt ttgctgaaaa tgaatgctgc cgatggaagt tatcttgcgc atcgcttatt
     1021 cgatgtgccg aatcctgatg ttggacgttc ttcgttttat gcctgctacg gcagaacgct
     1081 ggcgatagat acagcgggcc gattgcacct gctgggaaac attggatatg aagctgattt
     1141 tgaaaatgat acgctccatt cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnctggctta tcaaccgtga atgccgtgtc ggatcatttt actccgctta catggacggc
     1261 ttatcttgcg cccaatcctg cttcggaaac ggtcaggctt cattttcatt cacagcaagc
     1321 ggaaacgctg acggttacca tatcggatat actcggaaaa cgcctcgcat tgtttcaaaa
     1381 aatgaacata caaccaggtg aacaaacgct tgaacttccc attgataccg gggcgcctgc
     1441 tggtatttat tgggcgcggt tacaagcgga tgacggagca gtgcttatgc ttaagttggt
     1501 aaaaaaataa tgcaatgctg catacatgcg ctcgcaattt ctcagatggc ctatgacggt
     1561 tttagggccg taatgcggaa ttnnnnnnnn nnnnatttgc cttacttttg cccccacaat
     1621 caatctggat tcatgtccga tattggaaaa acagtaggcg tggtaggcgc cggcagcttt
     1681 ggttcggcca tcggcaacct gttggcgcac aatgtggacg tgctgctcta cagccgccag
     1741 ccggaattgg tggaggccat taatactacc caccagcact ttggcgtggc cctgtctcac
     1801 cgcgttcggg ctacctcgca cattgaagaa atggccgacc aatgccgcct catcatacct
     1861 gtagtgccct ctatgcgctt ccgcgaaatg atgcaacgcc tggggccaca cttgcgccct
     1921 taccatgtac tcattcatgc caccaaaggc ttcgacctgc acgatacccc
//
LOCUS       DAORLF010000637         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30676_length_1962_cov_1.954903, whole genome shotgun sequence.
ACCESSION   DAORLF010000637 DAORLF010000000
VERSION     DAORLF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30676_length_1962_cov_1.954903"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..264)
                     /locus_tag="PK198_27170"
     CDS             complement(37..264)
                     /locus_tag="PK198_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42513.1"
                     /translation="MRRFFKLTLAAALPALPTLASAHAGHSEFPAGHILHYLATPVHA
                     MPLLMAAVPIVVSAVAIRKELAVRKIRNRKD"
     gene            467..1579
                     /locus_tag="PK198_27175"
     CDS             467..1579
                     /locus_tag="PK198_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRF42514.1"
                     /translation="MVPTDTKRPTYYEELKERGYSRRDFMKFCTMMAAYLGLEASGTA
                     QIAKTLSKTSRVPVIWLHFQECTCCSESFIRSSHPIVADIILDKLSLDYTETLMAASG
                     HQAEAAREKTMKDYYGEYVLCVEGSVPTAENGAYCCIGGKSAEQILQEAAAKAVIAWG
                     SCASNGCVQSAKPNPTSATPIHKLISGKPLIKVPGCPPIGEVMAGVLVHIITFGTLPE
                     LDRVGRPKAFYSKRVHDSCYRRPFYDAGLFVETFDDENAKKGYCLYKVGCKGPMTYNS
                     CGIMRWNAGVSYPIQSGHGCFGCSEENFWDNGPLYRQQASFPGFGIESNADKVGMAAA
                     GITAAGVAAHAIVTNIQKKKLIANVINEGKESEKEL"
     assembly_gap    1632..1698
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1763..>1962
                     /locus_tag="PK198_27180"
     CDS             1763..>1962
                     /locus_tag="PK198_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF42515.1"
                     /translation="MAQRIVIDPITRIEGHLRIEAEIKDGKITDVYSSGTMVRGIEKI
                     VQGRDPRDVWAFVGRVCGVCTTV"
BASE COUNT          460 a          474 c          583 g          378 t
ORIGIN      
        1 ggggcgcccc aaaaatgtta gctatacccc aaaaaatcaa tctttccgat ttctgatttt
       61 gcgaacggcc agttctttgc gaatggcgac tgccgatacg acgatgggca cggcggccat
      121 gagcaggggc atggcgtgta cgggcgtggc gaggtagtgc aggatatggc cggcgggaaa
      181 ttcgctgtgg ccggcgtgtg cggaagcgag tgtgggcagg gccggaaggg cggcggcgag
      241 tgtgagtttg aaaaagcgac gcatgcgtgg tattttttag atgtgacaga gaagcgcgta
      301 aaagtagaaa atgtgccccg gcgaatagaa tgacaaaaat catgtatcgg gctgacaaat
      361 gtcagcaaag gctgtttgga catgcggtta ctttgcccca ttactgcaaa gtatgtcttt
      421 ttttcgagcg tccacattca cctaacaaac aaaaaacaga gcagacatgg tacccaccga
      481 caccaagcgt ccaacctatt atgaggaact gaaggagcgg ggctactcgc gccgcgattt
      541 catgaagttt tgcaccatga tggcggccta tctgggcttg gaggccagcg ggacggcgca
      601 aatagccaaa acgctgtcga aaacatcgcg ggtgccggtg atctggctgc actttcagga
      661 gtgtacctgc tgtagcgagt cgtttattcg ctcttcgcac cccatcgtag cagatattat
      721 attggataaa ttgtcgctgg actacaccga gacgctgatg gccgcatcgg ggcatcaggc
      781 agaggcggcc cgcgaaaaaa cgatgaaaga ctactacggc gagtatgtgc tgtgcgtgga
      841 aggctcggtg cctactgccg agaacggggc ctactgctgc attggcggca agtcggctga
      901 gcagattttg caggaggccg cagccaaggc cgtcattgcc tggggcagtt gtgccagcaa
      961 cggctgtgtg caatcggcca agcccaaccc cacctctgct acgcccatcc acaagctcat
     1021 atcgggcaaa ccgctcatca aggtgcccgg ctgcccgccc atcggcgaag tgatggccgg
     1081 ggtgctggta cacatcatca ccttcggcac actgcccgaa ttggatcggg tgggccgtcc
     1141 gaaagccttc tattccaagc gtgtacacga cagttgctac cgccgtccgt tctacgacgc
     1201 cggtctgttt gtggaaactt ttgacgatga aaacgccaag aaaggctact gcctgtacaa
     1261 ggtgggctgc aaaggcccga tgacgtacaa ctcttgcggc atcatgcgct ggaacgccgg
     1321 tgtgagctat ccgattcagt cggggcacgg ctgttttggt tgcagcgagg agaatttctg
     1381 ggacaacggg ccgctgtatc gtcagcaggc gtctttcccc ggtttcggga tagagagcaa
     1441 cgccgataag gtcggcatgg ccgccgcagg cattaccgcc gccggagtgg ctgcacacgc
     1501 cattgtgacc aatatccaga agaaaaaact cattgccaac gtgatcaacg aagggaagga
     1561 gtcggagaag gaactttgag cggtaggcag tgggcggtag gcggtaggca gtgggcagtg
     1621 ggcggtaggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnncg gtggataaaa ctcgaaactt ggaacaaaag attgaaaatc
     1741 atctatttaa aaaataccag acatggcaca acgcatcgtc attgacccta tcacccgcat
     1801 agaggggcac ctgcgcatag aagcagaaat caaagacggc aaaatcaccg atgtatatag
     1861 ctccggcacc atggtgcgcg gcattgaaaa gatagtgcag ggccgcgacc cgcgcgatgt
     1921 gtgggcgttt gtggggcgcg tgtgcggagt ttgcaccacg gt
//
LOCUS       DAORLF010000638         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30690_length_1961_cov_1.787513, whole genome shotgun sequence.
ACCESSION   DAORLF010000638 DAORLF010000000
VERSION     DAORLF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30690_length_1961_cov_1.787513"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>283)
                     /locus_tag="PK198_27185"
     CDS             complement(<1..>283)
                     /locus_tag="PK198_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42516.1"
                     /translation="VASCSKEQVVEQSANAEQPLSRQEINAFIEQSIQHTNDVFRWEN
                     ADLKLLWSAGVRSDSVFALGYQLPGEEDLKTRFHLIDPQSGAWAELRNRL"
     assembly_gap    284..376
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            696..>1961
                     /locus_tag="PK198_27190"
     CDS             696..>1961
                     /locus_tag="PK198_27190"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRF42517.1"
                     /translation="MKRYLLAALIAVMVSGMAYTQETPEHKNLFGAKVLFIGYGIPND
                     DDNLKITNGLELSYVRNLGKSLNVAVPLKVGLAHVPGEINNRTFGSLDAMLQLKAPGK
                     TIIPYLTAGAGVMAAQSDNVHLQIPVGGGVNFRVGSNSFVNLQGEYRIATLDQRNHLQ
                     AGLGVLFRLGRMADADGDGITDAMDACPNDPGPEATKGCPDKDGDGIADKMDRCPDLK
                     GSPEMQGCPDSDGDGFIDPEDECPQTAGQIKGCPDTDGDGLTDNKDKCPTEAGPESNM
                     GCPIPDRDNDGTPDADDRCPDVAGLAHLQGCPDRDGDGVSDIDDRCPDKPGPYSGCPD
                     TDGDGIMDADDRCPMEVGPASNRGCPEIKEETKKVLTTAMRAVQFETAKAVLKKESYP
                     ILDQVVMVLREYPSYHLRISGHTDNVGMAD"
BASE COUNT          431 a          488 c          547 g          402 t
ORIGIN      
        1 gaggcggttg cgaagctcgg cccaggcgcc actttgcggg tctatgagat ggaagcgggt
       61 tttgaggtct tcttcgccgg gcagttggta tcccaaagca aagacggaat cgctgcgcac
      121 gccggcgctc cagaggagct tgaggtcggc gttttcccag cggaatacat cgttggtgtg
      181 ttggatgctt tgctcgatga aggcgttgat ttcctggcgg gagagcggtt gttcggcatt
      241 ggccgattgc tcaacaactt gttctttgga gcaggaggcc accnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnagga gagtgcgggt aaaaatttaa tatctacttc ttcgtgaacg
      421 gctcatttca ctgagagaag ggagcattgc gtgagggggc tgaggggagc gacgtgagga
      481 gcgaccgaaa cacccgacgg cactgtccga ttgaaccgga gtgccggcac gcccaaagtc
      541 attgtgctga ttgttttttt catgcgcgcc ccttttacag tgtgcaaagt tggttacttt
      601 tgcctttcgc gaagtctaaa gcccggcagc gggagacgac aagaagcccg aatgctgtcg
      661 agtacttttt caaaaaccgt tttaattcct attttatgaa acgttatcta ttggcagccc
      721 tgatcgccgt gatggtgtcg ggtatggcct acacacagga aactcctgaa cacaaaaacc
      781 tctttggcgc caaagtgctg ttcatcggct acggtattcc aaacgatgat gacaatctga
      841 agattaccaa tggcttagag ctttcttatg ttcgcaattt aggcaaaagc ctcaacgtag
      901 cggtgccgct caaagtgggg ctggctcatg tgccgggcga gatcaacaac cgcacgttcg
      961 gaagtttgga tgccatgttg caactgaagg cgccgggtaa aaccatcatt ccctatctga
     1021 cggccggcgc cggtgtgatg gctgctcaat cggacaatgt tcacctgcaa attccggtag
     1081 gcggcggagt taatttccgt gtgggcagca attcttttgt gaacctgcaa ggcgaatacc
     1141 gcatagctac gctggatcaa cgcaaccatc tgcaagccgg cttgggcgtg ctgttccgct
     1201 taggcagaat ggccgatgcc gacggcgatg gtatcaccga tgccatggat gcctgtccca
     1261 atgaccccgg tccggaagct actaagggct gcccggataa agacggcgac ggtattgcgg
     1321 ataaaatgga ccgctgcccc gatctgaaag gctcgcccga aatgcaaggc tgccccgact
     1381 cggacggcga cggatttata gaccctgaag atgagtgccc gcaaacggcc ggtcagatca
     1441 aaggatgccc tgataccgat ggagacggcc tcaccgataa taaagacaag tgtcctaccg
     1501 aagccggccc cgaatcgaat atgggctgcc cgatacccga ccgcgacaac gatggtacgc
     1561 ccgatgccga cgaccgctgc cctgatgtgg ccggacttgc tcatctgcaa ggttgccccg
     1621 accgcgatgg cgacggcgtt tcggacatag acgaccgctg cccggataag cccggcccct
     1681 attccggctg cccggataca gacggcgacg gcatcatgga cgctgatgac cgctgcccga
     1741 tggaggttgg cccagcttcc aacagaggtt gcccggaaat caaagaagaa accaaaaaag
     1801 tactgactac cgctatgcgc gccgtgcaat ttgaaacggc caaagcggtg ttgaaaaaag
     1861 agtcatatcc cattctggat caggtggtga tggttttgcg cgagtatccg tcctatcact
     1921 tgcgcatcag tgggcatacc gacaatgtgg gcatggcgga c
//
LOCUS       DAORLF010000639         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_30826_length_1952_cov_2.013706, whole genome shotgun sequence.
ACCESSION   DAORLF010000639 DAORLF010000000
VERSION     DAORLF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30826_length_1952_cov_2.013706"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>843
                     /locus_tag="PK198_27195"
     CDS             <1..>843
                     /locus_tag="PK198_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF42518.1"
                     /translation="AAWQSEKEIVDTVQEIKKEIEELELQAERAERNSDYETVAKIRY
                     GELQTRQAMLKAAEEKLEKLPEENRFTNQQVTANDIADVVSRWTGIPVAKMLQSEKEK
                     LLKLEDEISQRLIGQQEAVVAVSDAVRRSRAGLGDADRPIGSFIFLGPTGVGKTELAK
                     TLAEMLFDDEKAITRIDMSEYQERHTVSRLVGAPPGYVGYEEGGQLTEAVRRKPYSIV
                     LLDEIEKAHPDTFNILLQVLDDGRLTDNKGRVANFKNTIIIMTSNMGAETILENFEDL
                     DALGD"
     assembly_gap    844..1089
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1090..1457
                     /locus_tag="PK198_27200"
     CDS             <1090..1457
                     /locus_tag="PK198_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42519.1"
                     /translation="LGYEPQFGARPMKRVLQKEVINELSRLVLAGTFGPGDTIYIHTD
                     AKGLTFSKDPFPGAIHIPEAAGEDDEAETASPISDAQIADSTIPAEDRKRRLEDLDKA
                     TKDLLDAIDNIEPDEDPEG"
     gene            complement(1680..1925)
                     /locus_tag="PK198_27205"
     CDS             complement(1680..1925)
                     /locus_tag="PK198_27205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42520.1"
                     /translation="MTAEPVMAFEELAQFLANLSPRKVLAFKTSKRWQERMDFLLSKN
                     TDQGLSPEENREMEQFMLIEHVVQMAKTKALLRIAEK"
BASE COUNT          453 a          479 c          430 g          344 t
ORIGIN      
        1 gccgcctggc aaagcgaaaa ggaaattgtg gataccgttc aggaaatcaa aaaggaaatc
       61 gaggaactcg aattgcaggc agagcgcgcc gaacgcaaca gcgactacga aacggtggcc
      121 aaaattcgct acggcgaact ccaaacccgc caggccatgc tcaaggccgc cgaggaaaaa
      181 ttggaaaagc tacccgaaga aaatcgcttc accaatcagc aggtcaccgc caacgacatt
      241 gccgatgtgg tttcccgctg gacgggcatt ccggtggcca aaatgctgca aagcgaaaag
      301 gaaaaactcc tcaaattaga ggatgaaatc agccaaaggc tcatcgggca gcaggaggcc
      361 gtcgtggccg tgtctgacgc tgtgcgccgc agccgggccg gtttgggcga tgccgaccga
      421 cccatcggtt ctttcatctt cctcggtcct accggtgtgg gcaaaacgga acttgccaaa
      481 accctcgccg aaatgctctt tgatgacgaa aaagccatca cccgcattga catgagcgaa
      541 taccaggagc gccacaccgt ttcccgcctc gtgggcgcgc ctccaggcta tgtgggctac
      601 gaagaaggcg gccaactcac cgaggccgtg cgccgcaagc cctactccat cgtcctcttg
      661 gacgaaatcg agaaggcgca ccccgacacg ttcaacatcc tgttgcaggt attggacgac
      721 ggtcgcctca ccgacaacaa aggccgcgtg gccaatttca aaaacaccat catcatcatg
      781 acctccaaca tgggcgccga gacgattttg gaaaattttg aagacttgga cgcgctcggc
      841 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnna tctgggctac gaaccgcagt tcggcgcccg ccccatgaag cgcgtactgc
     1141 aaaaagaggt catcaacgaa ctctcgcgcc tcgtgctggc cggcacattc ggtcccggcg
     1201 acaccattta catccacacc gatgccaagg ggcttacgtt ttcaaaagac cccttccccg
     1261 gcgccataca tatacccgaa gccgccggcg aagacgacga ggccgaaaca gcatcgccca
     1321 tttcggatgc gcaaattgcc gattccacca tccctgccga agaccgcaag cgccgcttag
     1381 aagatttgga caaagccacc aaagacctcc ttgatgccat agacaacatc gaaccggatg
     1441 aagacccgga gggctgagaa aagtagggag caaccagact atgggtaaag accagcaacg
     1501 gtaagcattg ccctgatttc gacgcgctct cgaaggttaa attcaaggag tttcgaagtg
     1561 gctttcccaa cttgtgtttt cggcattatt tgaaacggct ttgggaattc tgcaatactc
     1621 gcagcgattt ccagcacgtt caatcacaaa atggcggact atgcgtgaaa tataagcact
     1681 catttttcgg caattcgcag tagtgctttg gttttcgcca tttgaacaac atgctcgata
     1741 agcataaact gttccatctc gcggttttct tctggcgaaa gcccttggtc ggtattttta
     1801 gacaaaagaa aatccattcg ttcctgccac ctcttggagg ttttgaaggc gagaacttta
     1861 cgaggggaaa ggtttgccaa aaactgcgcg agttcttcaa atgccattac cggttctgct
     1921 gtcatctgct tatggtattg aatgttttac aa
//
LOCUS       DAORLF010000640         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_32448_length_1869_cov_2.142227, whole genome shotgun sequence.
ACCESSION   DAORLF010000640 DAORLF010000000
VERSION     DAORLF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32448_length_1869_cov_2.142227"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK198_27210"
     CDS             <1..518
                     /locus_tag="PK198_27210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42521.1"
                     /translation="RLTTSSMGRLFDGAAALLGLSLRSSYEGEAAMLLENSALEAFKK
                     YGIGENFGIEAAASVGLSAEDVLLPLAEGLRLGESREVLAAQFHLRLVRWAERMAAEQ
                     KVRYVVCSGGVFQNGLLADLISDRLSHHHTVLFHRQLSPNDESIALGQLMLHQSHKGN
                     NNPIKIISLAR"
     gene            complement(464..>989)
                     /locus_tag="PK198_27215"
     CDS             complement(464..>989)
                     /locus_tag="PK198_27215"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRF42522.1"
                     /translation="SLYLKRLPLLGNVLIALLCAAVPGMVWLLEQQAFVQLFATDVAA
                     GQRLKIVFLWYMASAFVLTLYRELVKDMEDVKGDSATGLRTLPVIWGVSATRYLAVVF
                     VLVSALVVGLMLWQLQTELHAWKQIGYGLSVLLPILVSILLLFRARTAADYRRLSHLA
                     KLIILMGLLLPLWL"
     assembly_gap    990..1023
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1024..1410)
                     /locus_tag="PK198_27220"
     CDS             complement(<1024..1410)
                     /locus_tag="PK198_27220"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRF42523.1"
                     /translation="MSRRVLNTSVFSLIRWPNLLIAGLVQYLLYFKVLQPVLAQFGIS
                     HSLDLLRLSMLVVMTLCVAAGGYIVNDIADVRTDEINRPARVVISKHLSHQTAYWLYF
                     MFQIVGFTLWLYLVFYVRRIPLLSVFP"
     gene            complement(1414..>1869)
                     /locus_tag="PK198_27225"
     CDS             complement(1414..>1869)
                     /locus_tag="PK198_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRF42524.1"
                     /translation="GVLTDGTVFIEEDGRLLRTMHVRDGLAMRTAVNKGYRVAIISAG
                     KSEGVKIRLEALGIEDVLMGQEDKVAAFDGLVAKYGLNPDQTLYMGDDLPDYKVMRKV
                     GMPACPADAAQEILELARYISPVPGGRGCVRDVIEKTLRLRGDWPIQND"
BASE COUNT          441 a          478 c          527 g          389 t
ORIGIN      
        1 cgcggctgac tacttccagt atggggcgct tgttcgatgg ggcggcggca ctgctgggct
       61 tgtctttgcg ttcgtcttat gagggagagg cggctatgtt gctggaaaac agtgcattgg
      121 aagcatttaa aaaatatggc ataggagaaa acttcggcat tgaggcagct gcttcggtcg
      181 ggctgtcggc ggaggatgtg ctgttgcctt tggccgaggg gctgcgattg ggcgaaagcc
      241 gggaagtatt ggcggctcag tttcatttgc gattggttcg ctgggcggaa cggatggctg
      301 cggagcaaaa ggtgcgctac gtcgtttgct ccggcggggt tttccaaaat ggcctgctgg
      361 cggatttgat ttcagataga ttaagtcatc atcataccgt gttgtttcac aggcagttgt
      421 cacccaatga tgagagcatt gcattggggc agttgatgtt gcatcaaagc cacaagggca
      481 acaacaaccc catcaaaata atcagtttgg cgagatgact caggcgtcgg taatcggcgg
      541 ctgttcgggc gcggaacagc aacaggatgg aaaccagaat cggcagtaaa accgatagcc
      601 cgtagccaat ctgtttccat gcgtgcagct cagtttgcaa ttgccacagc atgagtccga
      661 caacaagagc cgataccagc acaaaaacaa ccgccaaata ccgcgtcgcc gaaacgcccc
      721 atatcaccgg cagggtgcgc aaaccggtag ctgaatcgcc cttcacatct tccatgtctt
      781 tcaccaattc ccggtagagc gtgagtacaa acgccgaggc catgtaccat agaaacacaa
      841 tttttaacct ttggccggct gctacatccg ttgcaaataa ttgcacaaat gcctgttgct
      901 caagcaacca caccattcca ggtacggcgg cgcagagcaa agcaatgagc acatttccaa
      961 gcaaaggcag gcgtttgagg tacagcgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnaggaaaa acgctcagca gcgggatgcg gcgcacataa aacacaagat acaaccacaa
     1081 cgtgaagccc acgatttgaa acatgaagta caaccagtag gccgtttggt gagagaggtg
     1141 tttgctgatg acgacgcggg cagggcggtt gatttcgtcg gttctgacat ccgcgatgtc
     1201 attgacgatg tagccgccgg cagcgacgca caaagtcata accaccagca tggaaagccg
     1261 gagtaggtct aatgaatgtg aaatgccgaa ctgagccaaa accggctgca ataccttgaa
     1321 atacagcaga tactgtacca aaccagctat caacagattg ggccatctga tgagggaaaa
     1381 gaccgatgtg ttgagtaccc gccgcgacat gcctcagtcg ttttgtatgg gccagtctcc
     1441 ccgcagccgc agcgtttttt caataacgtc gcgcacgcag ccgcggccgc ccggaaccgg
     1501 cgagatgtac cgggccaatt ccagaatttc ctgcgcggca tcggcggggc aggccggcat
     1561 gcccactttg cgcatcacct tatagtcggg caggtcgtcg cccatgtaca gggtttggtc
     1621 gggattgagg ccgtatttgg ctaccaaacc gtcgaacgcg gctactttgt cttcctggcc
     1681 catgagcacg tcctcaattc cgagtgcttc gagcctgatt tttacgcctt cggatttgcc
     1741 ggcggagatg atggccaccc ggtagccttt gttcacggcg gtgcgcatgg ccaacccgtc
     1801 gcggacgtgc atggtgcgca ggaggcggcc gtcttcttcg ataaaaaccg tgccgtcggt
     1861 cagcacgcc
//
LOCUS       DAORLF010000641         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33005_length_1845_cov_1.443017, whole genome shotgun sequence.
ACCESSION   DAORLF010000641 DAORLF010000000
VERSION     DAORLF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33005_length_1845_cov_1.443017"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK198_27230"
     CDS             complement(<1..226)
                     /locus_tag="PK198_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42525.1"
                     /translation="MEKQLADEMEKKFWDALQEDLEQNRQNPLPYKYQEPEEAWRKLK
                     KITGPEDFDASPATGIDRKHIEHIVNHLQSI"
     assembly_gap    288..410
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<411..>710)
                     /locus_tag="PK198_27235"
     CDS             complement(<411..>710)
                     /locus_tag="PK198_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF42526.1"
                     /translation="ADILYNSEYDRILPILIHGDASVAGQGIVYETIQMSKLKGYLTG
                     GTIHLVINNQVGFTTDWDDARSSTYCTGVASVVHAPVFHVNGDDPEAVLFVSEMA"
     assembly_gap    711..722
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<723..1653)
                     /locus_tag="PK198_27240"
     CDS             complement(<723..1653)
                     /locus_tag="PK198_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRF42527.1"
                     /translation="MSDFSFISNAHPAFIDSLYQQYQQDPEVVEASWQLFFKGFDFGA
                     NGNGAAAVDAEHIQNELRVLSLIFAYRNRGHLESTTNPLKPRVNRQPYLAITDFGLTE
                     NQLNTPFRAGAEIGLHNATLGQIVAHLRKVYCSNIGFEFHSIQGREKRRWLRDYIEKS
                     APERQFDIPLEKKRRILEKLNGAVVFEQFLHTKFLGQKRFSLEGGETAIVSLDAIINK
                     ATESKVEEVVIGMAHRGRLNVLANIMGKTYKQIFTEFEGSNYKELSFGDGDVKYHLGF
                     SSQVGNLEGKTVHLKLVPNPSHLESVNPVVEGFA"
BASE COUNT          382 a          407 c          469 g          452 t
ORIGIN      
        1 gaatactttg caagtgattg acaatgtgtt caatgtgttt acgatcaatg cccgtagcgg
       61 gcgaggcgtc aaaatcttcc ggcccggtga tttttttgag tttgcgccaa gcctcttccg
      121 gctcctgata tttgtagggg agcgggtttt gtcggttttg ttcgaggtct tcctgaagtg
      181 cgtcccagaa ctttttctcc atttcatcgg cgagttgctt ttccacatct ccgcgctgta
      241 tcaatgtatc tgaatacatt tcacgggggg tggcgtggtt tttaatcnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgccatttc
      421 cgacacaaaa agcaccgctt cggggtcgtc gccattgaca tggaacaccg gcgcgtgtac
      481 taccgaggcc acacctgtgc agtaggtaga actgcgggcg tcgtcccagt cggtcgtaaa
      541 tccaacctga ttgttgatga ccaagtgaat ggtgccgccc gtgagatatc ccttgagctt
      601 gctcatctga atggtttcat acacgatgcc ctggcctgcc acggatgcgt cgccgtgaat
      661 gagaatgggc aaaatgcggt cgtattcgct gttgtagagg atgtcggctt nnnnnnnnnn
      721 nngggcgaat ccttccacga cggggttgac cgattcgagg tgggaggggt tggggaccaa
      781 tttgagatgt acggtcttgc cttccagatt gccgacctgc gaggaaaaac cgaggtggta
      841 cttcacgtcg ccgtcaccga aactgagttc tttgtaattg gacccctcaa actcggtgaa
      901 gatttgcttg taggttttgc ccatgatatt ggccagcaca ttgagacggc cccggtgcgc
      961 catgccgatg acgacttctt ctactttgct ctcggtggcc ttgttgatga tggcgtccaa
     1021 agagacaatg gccgtttcgc cgccttcgag cgagaatcgc ttctgcccca gaaacttggt
     1081 gtgcagaaac tgctcgaaca ccacggcgcc gttgagtttt tcgagtatgc ggcgcttttt
     1141 ttccaatggg atgtcgaact gcctttcggg agcgcttttt tcaatgtaat cgcggagcca
     1201 gcggcgtttt tcacggccct gaatgctgtg aaactcaaag ccgatattgc tgcaatatac
     1261 tttgcgaaga tgcgccacaa tctggccgag cgtggcattg tgcaaaccta tttctgcacc
     1321 ggctctaaat ggcgtattca attgattttc agtcaatccg aagtctgtga tggccaagta
     1381 aggctgccgg ttgacgcggg gtttaagcgg gttggtggta gattccaggt ggcctctgtt
     1441 gcgataggcg aagatgagag acagcacccg caattcgttc tgaatatgct ctgcatcaac
     1501 cgccgcagcg ccgttgccgt tggctccaaa atcaaagccc ttgaagaaga gttgccaaga
     1561 ggcttctacc acttcggggt cctgttgata ctgttggtat agggagtcaa tgaacgccgg
     1621 atgggcgttg gatatgaaag aaaaatcgct catggtttgg agattgtcac tttgcttaaa
     1681 aaatcgggca aatatcgctc aacgccgttg gatgaccaaa caacgaaggc gagattgaaa
     1741 cattattagt ggaaaaaaga tcaggttgac cgaatgagga aaatcgcttt tcagtcaagt
     1801 tgcgcatcaa aatccaccaa acggacaaac tgtccgatgc gttct
//
LOCUS       DAORLF010000642         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33299_length_1832_cov_2.468768, whole genome shotgun sequence.
ACCESSION   DAORLF010000642 DAORLF010000000
VERSION     DAORLF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33299_length_1832_cov_2.468768"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1379)
                     /locus_tag="PK198_27245"
     CDS             complement(<1..>1379)
                     /locus_tag="PK198_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF42528.1"
                     /translation="PMGISATTKAVPVAQVARDAGINLHWSPDSKRLHWTLGDEYYSE
                     ELSRRFKFLEGAPDTIPPIDTVGIKIGLKLKSDKPEGKIALRNARIITMKGSEVIEKG
                     TVLIEGNKIVAVGPASRVVIPRDAKVIDCTGKTIMPGIIDVHGHLGNFRYGISPQQQW
                     HYFANLAYGVTTAHDPSSNSEMIFSQSEMIKAGSMIGPRLYSTGNILYGADGDFKAVI
                     NNLDDARSALRRTAAYGAFSVKSYNQPRRDQRQQVMQAAREQGIIVVPEGGSFFYHNL
                     SQVADGHTGIEHNIPVAPLYDDVVKFWSQTKAHNTPTLIVNYGGLNGEYYWYQHTNVW
                     EKERLLRFTPRAVIDSRARHRTMAPEEEYKNGHILTSQSCKKLQDAGVNINLGAHGQL
                     QGLGAHWELWMLQQGGMSNMQALRSATLNGAVYLGMDKEIGSIETGKLADLLVLDKNP
                     LENIRNSEF"
     assembly_gap    1380..1407
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1408..>1832)
                     /locus_tag="PK198_27250"
     CDS             complement(<1408..>1832)
                     /locus_tag="PK198_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF42529.1"
                     /translation="LRKLNLATGQSAKLTTEKGIYRTPNFSPDGKTIVYRKEGGNDHQ
                     GFTHSKEPGIYTIPAAGGKPAFVTRQGEFPQFNTDGTRIFVQTGGYLFGSLSKTMKSF
                     KLDGTDEKTVFTTQYTNQFVPSPDNKWVAFTELYKVYIA"
BASE COUNT          310 a          464 c          581 g          449 t
ORIGIN      
        1 gaactccgag ttgcggatgt tctccaaggg gtttttgtcc aacaccagca ggtcggccaa
       61 tttgccggtt tcgatggagc cgatttcttt atccatgccc agatacaccg cgccgttcag
      121 cgtggcgctg cgcagcgcct gcatattgct catgccgcct tgctgaagca tccacagttc
      181 ccagtgtgcg ccgaggccct ggagttggcc atgcgcgccg aggttgatgt tcacgccggc
      241 atcctgtagt tttttgcacg attgcgaagt gaggatgtgg ccgtttttgt actcctcttc
      301 gggggccatg gtgcggtgcc gcgcccggct gtcaatgacg gcacggggcg tgaagcgcag
      361 caggcgttcc ttttcccata cgttggtgtg ctggtaccag tagtattcgc cgttgaggcc
      421 gccgtagttc acgatgagcg tgggcgtgtt gtgggctttg gtttgcgacc agaatttcac
      481 cacgtcgtcg tacagcggcg ccacggggat gttgtgctct atgcctgtgt ggccgtcggc
      541 tacctgactg aggttgtggt agaagaagga gccgccttcg ggcaccacga tgatgccttg
      601 ttcgcgggcg gcctgcatga cctgctggcg ctgatcgcgg cgcggctgat tgtaactctt
      661 caccgaaaaa gcaccatacg cggcggtgcg gcgcagggcc gagcgggcgt cgtctaagtt
      721 attgatgacg gctttaaagt cgccgtcggc gccgtagagg atgttgccgg tagagtacag
      781 gcgcgggccg atcatgctgc ctgctttaat catctcggat tgcgaaaaga tcatctccga
      841 attggaagaa ggatcgtggg cggtggtgac gccataagct aagttggcga agtagtgcca
      901 ctgttgctgc ggactgatgc cgtagcggaa attgccgagg tggccgtgta cgtcaatgat
      961 gcccggcatg atggttttgc cggtgcaatc aatcactttg gcgtcgcggg gaatgaccac
     1021 gcgggaagcc gggccgaccg ccacgatctt gttgccctct atcagtaccg tgcctttttc
     1081 gatgacttcg ctgcccttca tggtgatgat gcgggcgttg cgcagggcga tcttgccttc
     1141 gggcttgtct gacttgagtt tcagcccgat tttgatgccc accgtatcaa tgggcgggat
     1201 ggtatcggga gcgccctcga ggaatttgaa gcggcgagag agttcttcgc tgtagtactc
     1261 atcgcctagg gtccagtgca ggcgcttgct gtcgggactc cagtgcaggt tgatgccggc
     1321 gtcgcgggcc acctgcgcca caggcacggc tttggtggta gcgctgatgc ccatgggctn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnngag caatatacac tttgtacaac tcggtgaaag
     1441 ccacccactt attgtcagga ctgggtacaa actgattggt gtattgcgtg gtgaaaactg
     1501 ttttttcgtc ggttccgtcg agtttgaaac tcttcatcgt tttgctcaaa ctgccgaaga
     1561 ggtagccgcc ggtttgtaca aaaatgcgcg tgccgtcggt gttgaactgc gggaactcgc
     1621 cctgacgcgt cacgaaagcg ggcttgccac cggctgccgg aatggtgtaa atgccgggtt
     1681 ctttgctgtg cgtgaaaccc tgatgatcgt tgccgccttc cttgcgatac acgatggttt
     1741 tgccgtcggg cgagaagttc ggcgtgcgat aaatgccttt ttcagtggtg agttttgccg
     1801 attggccggt agcgaggttg agcttgcgca gg
//
LOCUS       DAORLF010000643         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33410_length_1827_cov_1.892212, whole genome shotgun sequence.
ACCESSION   DAORLF010000643 DAORLF010000000
VERSION     DAORLF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33410_length_1827_cov_1.892212"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>301
                     /locus_tag="PK198_27255"
     CDS             <1..>301
                     /locus_tag="PK198_27255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42530.1"
                     /translation="EKRIDFAEPSSSYQIPFAAFYADCEHEVEPVTSGYRVCLVYNLV
                     QQSKSTRIRLPQLSAQTAEIAQLLKTGEASFTEFPAAVLLGHQYTPANFSLAQLK"
     assembly_gap    302..494
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <495..1780
                     /locus_tag="PK198_27260"
     CDS             <495..1780
                     /locus_tag="PK198_27260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42531.1"
                     /translation="KYTTIEHWADDDERPSLGHFSIRDEDIISDVKIGEGEPDEQEEE
                     GYTGNEGMTIEYWYHYGAVLIWPVRIHGKVLSQIDIGAQLQWLDFYAGNWREPQLEAP
                     RHVKTMLQLMNQSLSERTERIHRTADYSPVAAALAQLQDEPFLRKTGLQILTKVFYQI
                     QLKQWIGLMRPYAPALFHPVFRETADSQTPEVWRHLLETLVGLDKAGFTEFVSEQMKT
                     LPPLLKKAVFYKMPESSYTYNWDNQQSERNAILCIAMKKLLSLCHYCDADALWREQVV
                     DAIFGTLPDRKYVNDALLPALLAGKYRSDALAADLRRACREDLQIRTAVKPTPPPDWK
                     RETPSDRLHKKVWDLLRPFLSSPTEQVFDYRAAEGLRREMEYAVKSVTIDLRMDTLTS
                     GRPYTLRLTKTQAAYERALAEWWEDVGWLEKVGDY"
BASE COUNT          419 a          465 c          434 g          316 t
ORIGIN      
        1 gggaaaagcg catagacttt gccgagcctt cgagcagcta ccagattccc ttcgctgctt
       61 tttatgccga ctgcgagcac gaggtggagc cggttacgtc gggctaccgg gtttgtctgg
      121 tttacaacct cgtacaacaa agcaaaagta cgcgcatccg cttgcctcag ctcagcgcgc
      181 agactgccga aattgcccaa ttgttgaaaa ctggcgaggc gagctttact gagtttcccg
      241 cagcggtact gctggggcac caatatacgc ctgccaattt ctcgctcgct caactcaaat
      301 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnaaaaat ataccaccat agaacactgg gccgacgatg atgagcgccc
      541 cagcctcggc catttcagca tccgcgacga agatatcatc agcgatgtaa aaataggtga
      601 gggagaaccg gacgaacagg aagaagaagg ctacaccggc aatgagggca tgaccatcga
      661 atactggtac cactacggcg ccgtactcat ctggcctgtg cgcatacacg gcaaggtact
      721 gagccaaata gacatcggcg cgcaattgca gtggctcgat ttttacgccg gcaattggag
      781 ggaaccccaa cttgaagcgc cgaggcacgt caaaacgatg ctccaattaa tgaaccaaag
      841 cctgtctgaa cgaacagagc gaatacaccg caccgcagat tacagtccgg tagccgccgc
      901 tctggcgcaa ttgcaggatg agccattcct ccgcaaaacc gggctacaga tactgacgaa
      961 ggttttctat caaatccaat tgaaacaatg gatcggcctg atgcggccat acgccccggc
     1021 gctgtttcat ccggtgttcc gggaaactgc cgacagccaa acgccggaag tgtggcgcca
     1081 cctactggaa acgctcgttg ggttggacaa agcgggattc acggagtttg tgagcgagca
     1141 aatgaaaaca ctgccgcctt tgctgaaaaa ggccgtgttt tataaaatgc ccgaatcatc
     1201 ctacacctac aattgggaca atcagcaaag cgagcgcaac gccatacttt gcattgccat
     1261 gaaaaagctg ctctcgctgt gccattattg cgatgcggat gccctatggc gcgagcaagt
     1321 agtggatgct atttttggca cgctgccgga tagaaaatat gtgaacgacg cgctgctccc
     1381 ggcactcttg gcgggcaagt atcggagcga tgccttagcc gccgatctgc gccgcgcctg
     1441 ccgcgaagac ctgcaaatac gcactgccgt aaaacccact ccgccccccg actggaagcg
     1501 cgaaacgccc tccgaccgac tgcacaaaaa ggtatgggac ctcctgcggc ccttcctcag
     1561 ttcgcctacc gaacaggtgt tcgactaccg cgcagcggaa ggcttgcgca gggaaatgga
     1621 atacgctgtc aaatcagtaa ccatagattt gcgcatggac accctcactt ccgggcggcc
     1681 ctacacgctg cggctcacca aaacgcaggc ggcctacgag cgcgccctgg cggagtggtg
     1741 ggaggatgtg gggtggttgg agaaggtggg ggattattga cttatcaact cccacacttt
     1801 cacaaaacat gtcaaccaac ataccat
//
LOCUS       DAORLF010000644         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33475_length_1824_cov_1.321651, whole genome shotgun sequence.
ACCESSION   DAORLF010000644 DAORLF010000000
VERSION     DAORLF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33475_length_1824_cov_1.321651"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>418
                     /locus_tag="PK198_27265"
     CDS             <1..>418
                     /locus_tag="PK198_27265"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42532.1"
                     /translation="TGAANGTGNSLDNSLFAGPGDNVLNGGGGTDTLSYFYATGPVSV
                     SLAAVGPQITGASGRDTLMSIENLYGGSFDDSLAGDARANRLDGGAGADSLVGHGGDD
                     ILVGGVGFDTFVFATNFGNDRINDFTHGQDQIQLILG"
     assembly_gap    419..436
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            752..>1553
                     /gene="wecB"
                     /locus_tag="PK198_27270"
     CDS             752..>1553
                     /gene="wecB"
                     /locus_tag="PK198_27270"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRF42533.1"
                     /translation="MKTILTVVGARPQFVKAASISRALKTHRCFREVIVHTGQHFDPN
                     MSDVFFDEMDIPKPDFDLNIHSLSHGAMTGRMMEGLEALMLGQKPDLVLIYGDTNSTL
                     AGALTARKLNIPVAHVEAGLRSFNMLMPEEVNRIISDRISSVLYCPTHQAKSNLLQEG
                     FANFAARILVSGDVMYDAAIFYAQKAASISRIREKLGLTHYILCTLHRAENTDNPERL
                     SSICRALDKLATQFQIALPLHPRTRQIMKRSRIELSFQPLDPVGYFDMI"
     assembly_gap    1554..1563
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1564..1786)
                     /locus_tag="PK198_27275"
     CDS             complement(<1564..1786)
                     /locus_tag="PK198_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42534.1"
                     /translation="MAVSIEAGFKAHWLLEHFLNGTNDLFLIWAGEGIRACLDQLNPF
                     RLVSYGYAGLLKKIRLFLQAAAVSEYKISS"
BASE COUNT          455 a          553 c          415 g          373 t
ORIGIN      
        1 ccactggcgc agccaacggt accggcaaca gcctcgacaa ttcccttttc gccggtcccg
       61 gcgacaatgt gcttaatggc ggcggcggca ccgacaccct gtcctacttc tacgccacag
      121 gaccggtatc ggtcagcctg gcagccgttg gcccacagat caccggcgct tccggtagag
      181 acacattgat gagcatcgag aacctctacg ggggctcctt cgacgacagt ctggccggag
      241 atgcccgcgc caacagactc gatggcggcg ccggcgcaga cagcctcgtc ggccatggcg
      301 gtgacgatat ccttgtcggg ggcgtcgggt ttgacacctt tgttttcgct accaactttg
      361 gcaatgaccg aattaatgac ttcacgcacg gccaggatca gattcagttg atactcggnn
      421 nnnnnnnnnn nnnnnnactt ctccgcactg gacaccaacg ccgatggcac actcggccat
      481 ggtgacgccc acgtccaaat tgcgggtccg gacacgttca tcgacttcgg taccgatcaa
      541 ctccgaatcg tgggatactc cgcattgcaa agcagcgact tcatcttctc atgagttgct
      601 ggccaagacc cgaacactcg gaatcacagt acaggccgtg cctagaacct tccaccagcc
      661 agcacaaacc cctatccata caaagaacca acgaaccgcc aaaatccctt cacaaccttt
      721 cactacccaa tcattaacga aaaacgcagc catgaagacc atcctaactg ttgtcggggc
      781 caggcctcaa tttgtcaagg cggcctccat cagtcgcgcg ctgaaaacac atcggtgctt
      841 ccgagaagtc atcgtccaca ctgggcaaca tttcgaccca aatatgtcag acgtcttctt
      901 tgacgaaatg gacatcccca aacccgattt tgatctcaac atccacagcc ttagccatgg
      961 cgccatgacg ggcagaatga tggaaggact tgaagcgctg atgctaggtc agaaacctga
     1021 ccttgtgctg atatatggcg acaccaactc aacacttgct ggcgccttaa cggcaagaaa
     1081 actcaacatc cccgtagcac acgttgaagc cggcctgcgc agttttaata tgttaatgcc
     1141 cgaggaagtg aaccgcataa tctccgaccg catttccagc gtattatact gccccactca
     1201 ccaagcaaaa tcaaatctac tgcaggaagg cttcgcgaat tttgccgcac gaattctcgt
     1261 ttcaggcgac gtaatgtacg acgccgccat attttatgct caaaaagcag cctcaatatc
     1321 tcgaatccgc gaaaaactcg gcctgaccca ctatatcctc tgcacccttc atcgcgccga
     1381 aaacaccgac aaccccgaga ggctttcctc tatttgccgg gcgctcgata aactcgcgac
     1441 gcagtttcag atcgcactac ccctacaccc gcgcacaaga cagattatga agagatcgcg
     1501 catagagcta tcttttcaac cacttgaccc cgttggctat ttcgacatga tctnnnnnnn
     1561 nnngagagct gatcttgtac tcactgacag cggcggcctg caaaaagagg cgtatttttt
     1621 tcagaagccc tgcgtaaccg taagagacca aacggaatgg gttgagctga tccaagcagg
     1681 cgcgaatacc ctcaccggcc cagatgagga acagatcatt cgtgccgttg aggaaatgct
     1741 ccaaaagcca atgtgctttg aatcccgcct ctatggagac ggccacgcgg cagagtttat
     1801 tgccaacgac ttggtcttgc atct
//
LOCUS       DAORLF010000645         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33648_length_1815_cov_2.117045, whole genome shotgun sequence.
ACCESSION   DAORLF010000645 DAORLF010000000
VERSION     DAORLF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33648_length_1815_cov_2.117045"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK198_27280"
     CDS             complement(<1..213)
                     /locus_tag="PK198_27280"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRF42535.1"
                     /translation="MKLPELLQAIEPVRTTGSLDREIGSVVFDSRKAAPGSLFVAVKG
                     LHNDGHAYLDKVVQNGVKAVVVQDWPD"
     gene            complement(227..>1422)
                     /locus_tag="PK198_27285"
     CDS             complement(227..>1422)
                     /locus_tag="PK198_27285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF42536.1"
                     /translation="LEDSVSIEKGRTKFYNDEMVDSSPFSATLDTITIQRAFEISSNV
                     GMAKLAQQYYGKKERINKNQGAARFIEHLKDFHLHLPTGVEVAGEGVPYIKEAYSSAH
                     EWSGTTLPWMAIGYELKITPLQLLTFFNAVANDGEMVKPYLVAEIQRDGATVQTFKPT
                     VVDRQIASKNTIRQAKMLLEGVVERGTAEKLRHPDTLYRFAGKTGTAQIGYRKVSNRT
                     TLIRGYQASFVGYFPAENPVYTCIVIINNPKANGFYGSDVAGPVFREIADYCYVTATS
                     AHKALNTDSKPVLAAKQLPNNNMGFTPDMSYLIETLELPHFGSPKTDWSVLRVRSDSL
                     VWEDRTIAENVVPNVTGMGLRDALYILENRGLKAAVNGFGKVTRQSIRPGTRARGQTV
                     QLYLN"
     assembly_gap    1423..1447
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1448..>1815)
                     /locus_tag="PK198_27290"
     CDS             complement(<1448..>1815)
                     /locus_tag="PK198_27290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF42537.1"
                     /translation="VYDNILGGRPGRQAMVCVDKVKDIWLPVNALTEVEPKRGDDVKT
                     TLDVNIQDVTQEALMRAMNYHNAEWGTAVVMEVKTGAIKAIANLGRTEEGWWETFNHA
                     VGTAIEPGSTFKLATMMSMM"
BASE COUNT          362 a          489 c          456 g          483 t
ORIGIN      
        1 atcgggccag tcttgcacca ccactgcttt tacaccgttt tgtacaacct tgtcaaggta
       61 ggcgtgtcca tcgttatgga ggccttttac tgccacaaac aaactaccgg gggcagcttt
      121 gcgcgaatcg aataccaccg aaccgatctc gcggtcgagg cttcctgttg tgcgaacagg
      181 ttcgatggct tggagcaatt ccggcaactt catgtcggca gcagttttaa tttaaataca
      241 attgtacggt ttgccctctg gcccgcgtac cgggccggat ggactgccgc gttacttttc
      301 caaaaccgtt gacggccgct tttaatcctc tgttttccag gatgtaaagg gcgtcgcgca
      361 ggcccatgcc cgtcacgttg ggcaccacat tttcggcaat ggtgcggtct tcccacacca
      421 agctgtccga acgcacgcgc aacacgctcc aatctgtttt tggacttccg aaatgcggca
      481 gttccagcgt ttcgatcagg tagctcatgt cgggcgtgaa tcccatgttg ttgttgggca
      541 attgcttagc ggccagtacc ggttttgagt ctgtattgag cgctttgtgc gccgatgtag
      601 ccgtcacata gcaatagtct gcaatttccc tgaagaccgg ccctgccaca tcgctgccgt
      661 agaagccatt ggctttggga ttgttgatga tcacgatgca ggtatatacc gggttctccg
      721 ccgggaagta acctacgaac gaagcctgat accctctgat gagcgtggtt ctgttcgata
      781 ctttgcggta gccgatctgc gccgtaccgg ttttgccggc aaagcggtag agcgtgtctg
      841 ggtggcggag tttttctgcc gtaccgcgtt ctactacacc ttccagcaac atcttggcct
      901 ggcgaatggt atttttggaa gcgatctggc gatctaccac tgtaggctta aaagtttgta
      961 ccgttgcccc atctctttgt atttcagcca ccaaatacgg tttcaccatt tcgccgtcgt
     1021 tggccactgc attaaagaac gtgagcaatt gtaaaggcgt gattttcaat tcgtaaccaa
     1081 tagccatcca gggcagagtc gttccgctcc attcgtgtgc gctgctgtaa gcctccttga
     1141 tataaggcac gccttcgcct gccacctcaa cgccggtggg caaatgcagg tgaaaatctt
     1201 tcaggtgctc aataaagcgg gcggcgcctt gatttttgtt gatgcgctct tttttgccat
     1261 aatattgctg agccagcttg gccataccta cgttggaaga aatctcgaag gcgcgttgga
     1321 tggtgatggt gtccaacgta gcgctgaacg gagaggaatc caccatttca tcgttgtaaa
     1381 atttggtgcg ccccttttcg atagaaaccg agtcttccaa ttnnnnnnnn nnnnnnnnnn
     1441 nnnnnnntcc atcatggaca tcatggtggc caatttgaac gtagagccgg gttcgatggc
     1501 cgttcccact gcatggttga acgtttccca ccaaccttcc tctgtgcgtc cgaggttggc
     1561 aatggctttg atggcgccgg tttttacctc cattactacg gcagtgcccc attcggcgtt
     1621 gtggtagttc atggcgcgca tcagggcctc ctgcgtcaca tcttgtatat tgacgtccag
     1681 cgtagttttg acatcgtcgc cccgcttagg ctccacttcc gtcaacgcat tgacaggcag
     1741 ccagatatcc ttcactttat ccacacacac catcgcctga cgccccggac ggccgccgag
     1801 aatgttgtca taaac
//
LOCUS       DAORLF010000646         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33674_length_1814_cov_1.997726, whole genome shotgun sequence.
ACCESSION   DAORLF010000646 DAORLF010000000
VERSION     DAORLF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33674_length_1814_cov_1.997726"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK198_27295"
     CDS             <1..210
                     /locus_tag="PK198_27295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42538.1"
                     /translation="IQILNLRREGLVAGRRRLVMTNGLLIWLKNGWFTARKKAHTHSG
                     SRKVAAGNRAIGFVPVTKRFTFVTF"
     gene            complement(275..>1569)
                     /locus_tag="PK198_27300"
     CDS             complement(275..>1569)
                     /locus_tag="PK198_27300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF42539.1"
                     /translation="TVIRDVFESFLPLAEKRGIHLSLRDTPLREAAQGALYLFDAGKL
                     ELILNNLVSNALKFTPSGGRVVIQAGLLNLGALVNLEISVGDTGIGIPAAALGKIFDR
                     FYQVDGTHTRSGEGTGIGLALSKELAELMGGSIRVESEPGRGSTFTFRIPVQPDQPVV
                     ATEEQAPDDRMAMPPISELQGEWPVVLVVEDNADLRGFIKNSIGAACQVVEASDGEEG
                     LQKALELLPDLVISDVMMPRKDGYDLCDTLKTNELTAHIPVILLTAKSTMDAKLKGLR
                     RGADDYLTKPFSTEELLARMYNLMALRRKLMERWGKRPSDAPTDTEDGLSGPDREFLK
                     KLTLLIEQHLSDETLGVEDFAGKMYISRVQLHRKLKAITGSTATDFIKNYRLDRAYSM
                     LRNREGLVGEIALRVGFGNEKYFSTVFKEKFGLPPSQV"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1580..>1814)
                     /locus_tag="PK198_27305"
     CDS             complement(<1580..>1814)
                     /locus_tag="PK198_27305"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF42540.1"
                     /translation="LEQMKTNFFSNVTHEFRTPLTLIMEPLRQLMKNPEAPDRQEKIR
                     LAENNSRKLLGLVNQLLDMAKLESGAMSLDLRGT"
BASE COUNT          361 a          483 c          499 g          461 t
ORIGIN      
        1 atccaaatct taaatttacg aagggaaggg ctggttgcag gcaggcgtcg gttagtcatg
       61 acaaatggtt tattaatatg gctgaaaaat gggtggttta ccgcccggaa aaaagcacat
      121 actcattccg gctcgcgcaa agttgcagcc gggaacaggg ctatcgggtt cgttcctgtt
      181 accaaaaggt ttactttcgt tacattttga agcggggttt gatctcgtta catcggggtt
      241 tgatctcgtt acatgtgtac tgctgcacag tagcttacac ctgactgggg ggcaggccga
      301 atttctcttt gaacacggtg gaaaaatact tctcgttgcc gaaacccacc cgcagggcaa
      361 tttcgcctac caggccctcc ctgttgcgga gcatggaata ggcccggtcg aggcggtagt
      421 ttttaataaa atcggtggcg gtgctgccgg tgatggcctt caattttcga tgcaattgca
      481 cccggctgat gtacatttta ccggcaaagt cttccacgcc caaagtctcg tcggagaggt
      541 gctgctctat cagcagggta agttttttca aaaactcccg gtcggggcct gacagtccgt
      601 cttcagtatc agtcggcgca tctgatggcc ttttccccca acgctccatc agcttgcggc
      661 gcagcgccat gaggttgtac atacgggcca gcaattcctc ggtgctgaac ggcttggtca
      721 ggtagtcgtc ggcgcccctg cgcaggccct tcagtttggc atccatcgtt gacttggccg
      781 tgagcaggat gacagggata tgcgcggtta gttcgttggt tttcaatgta tcacacaggt
      841 cgtatccgtc cttgcggggc atcatcacat ccgaaatgac gaggtcgggc aacaattcaa
      901 gggctttttg cagcccctcc tcgccgtccg aggcttccac tacctgacag gctgctccga
      961 tggaattttt gataaatccc cgcaaatcgg cattgtcttc caccaccagc actacgggcc
     1021 attcgccttg cagctcagat attggcggca ttgccatgcg gtcatcgggt gcttgctctt
     1081 cggtggcgac aaccggctga tcgggctgca caggtattct gaatgtgaag gtagaacctc
     1141 ttcccggctc gctttccacg cggatactgc cgcccatgag ttcggccagt tctttgctca
     1201 atgccagccc tatgcccgtg ccttcaccgc tgcgggtatg cgtgccgtcc acctgataaa
     1261 agcggtcgaa tatcttgccg agcgccgctg ccggtatgcc tatgcccgta tcgccgacgc
     1321 tgatctccag gtttactaaa gctccgaggt ttagtaaacc tgcctgaatg acaacccgcc
     1381 cgccggaggg ggtgaatttc aaggcattgg aaactaagtt gttgagaatc aattccaatt
     1441 ttcccgcatc gaagaggtac agtgcgcctt gcgcggcttc ccgtagcggc gtatcccgca
     1501 ggctcagatg aatgcctcgt ttttcggcca ggggcaaaaa ggactcaaac acgtctcgta
     1561 tcactgtgcn nnnnnnnnng gttccgcgca ggtcgaggct catggcgccg ctttcgagtt
     1621 tggccatgtc caagagttgg ttgacgaggc cgagcaattt gcggctgttg ttttcggcca
     1681 gcctgatttt ttcctgccgg tcgggagctt cggggttttt catcaactgc ctcagcggtt
     1741 ccatgatgag cgtgagcggc gtgcggaact cgtgggttac attggagaag aaattggttt
     1801 tcatctgctc caag
//
LOCUS       DAORLF010000647         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33727_length_1812_cov_1.564599, whole genome shotgun sequence.
ACCESSION   DAORLF010000647 DAORLF010000000
VERSION     DAORLF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33727_length_1812_cov_1.564599"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>344)
                     /locus_tag="PK198_27310"
     CDS             complement(<1..>344)
                     /locus_tag="PK198_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRF42541.1"
                     /translation="APAAVAGTGRDGRITKEDALKAVATPAPAPAAPAPVAPASPAAP
                     AAADHQAFSRAEDRKKMSRMRRTIAARLVKAKNETAMLTTFNEVDLSEVMALRSKYNE
                     RFEKKFGIKLGF"
     assembly_gap    345..399
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<400..726)
                     /locus_tag="PK198_27315"
     CDS             complement(<400..726)
                     /locus_tag="PK198_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRF42542.1"
                     /translation="MSLLEAKVPVIGESISEVTLSKWLKPNGAYVNIDESICEFESDK
                     ATLELPAEASGILIYVAKEGDDLEIGAVVARIDTAAARPADGAAPAPKAETPAPAAPA
                     SQPAEAT"
     assembly_gap    737..755
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(863..>1812)
                     /locus_tag="PK198_27320"
     CDS             complement(863..>1812)
                     /locus_tag="PK198_27320"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRF42543.1"
                     /translation="AMIEEMRRDETVFLMGEEVAEYNGAYKVSKGMLDEFGPRRVIDT
                     PIAELGFAGIGVGAAMNGLRPIVEFMTWNFAVLAFDQIVNNAAKTLSQSAGQFQCPIV
                     FRGPSGAAGQLAQQHSQTFESWMANVPGLKVISCIDPADAKGLLKSAIRDEDPVCIME
                     SEMLYGHTGEVPDGEYLVPIGVAAVRKQGTDVTLVSYNKMMLPTLEAAEILASEGISA
                     EVIDLRTIRPLDVRTIIESVKKTNRLVVIDESWPFAGVSSEVAYVVQRHAFDYLDAPI
                     MRINAADTSLGYAPTLVEEYMPNAAKIVKAVKEVMYVRY"
BASE COUNT          364 a          456 c          501 g          417 t
ORIGIN      
        1 gaagcccagt ttgatgccga attttttctc aaaacgctca ttgtatttgg agcgcagggc
       61 cattacctcg ctgaggtcca cttcattgaa agtagtgagc atggccgttt cgtttttggc
      121 tttcaccaac cgggcggcaa tggtgcggcg catgcggctc atctttttgc gatcctctgc
      181 acggctgaat gcctgatgat cggcagcagc gggtgcggca ggcgaggcag gagcgacggg
      241 agcaggagcg gcaggagcgg gcgcaggagt ggccactgct ttcagcgcgt cttctttggt
      301 aatgcggcca tctcttccgg taccggctac ggcagcagga gctannnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtggcttcag cgggttgtga
      421 agcgggggca gcgggagcgg gcgtttcagc tttgggggcc ggagcggctc cgtctgcggg
      481 gcgagcggcg gcggtgtcta tacgggccac cacggcgcct atttcgaggt cgtcgccttc
      541 tttggctaca tatataagaa tgccggaagc ctcggctgga agttcgagcg ttgctttatc
      601 tgattcaaat tcacagatag actcgtcaat attgacatag gcgccattgg gtttcaacca
      661 cttagacagg gttacttctg aaatggattc accgatgacg ggtactttgg cttcaagaag
      721 gctcatcgct gtatgtnnnn nnnnnnnnnn nnnnnatatc aaaaagtcaa tgttcaagat
      781 ttcggattcc cgatttcgga tttcggattc ccgatttcgg atttcggatt cccgatttcg
      841 gattcccgat ttcgcgttga agttagtacc tgacgtacat tacttctttc accgctttta
      901 caattttagc ggcattcggc atatattcct ccaccaaagt gggtgcatat cccaaagaag
      961 tatctgcggc attgatgcgc atgatgggag cgtcgaggta gtcgaaagca tggcgctgta
     1021 ccacatacgc cacctctgaa gacacgcccg cgaaaggcca ggactcgtca atgaccacca
     1081 gacgattggt ctttttgacc gactcaatga tggtgcgaac atccaatggg cggatggtgc
     1141 gcagatcaat cacctcggcg gagatacctt ccgaagcaag tatctcggct gcttcgagcg
     1201 tgggcaacat cattttgttg taagaaacga gcgtcacatc tgtgccctgc ttgcgcactg
     1261 ccgccactcc gatgggtacc aaatactcgc cgtcgggtac ttcgccggtg tggccgtaga
     1321 gcatttccga ctccatgatg caaaccggat cctcgtcgcg aatggccgat ttgagcaagc
     1381 ctttggcatc ggcgggatca atgcaggaaa tgaccttgag gccgggcaca ttggccatcc
     1441 aactttcgaa agtttgggag tgctgctgcg ccaactgtcc ggctgcgccc gaaggcccgc
     1501 gaaacacaat ggggcactgg aactggccgg cagattggga aagcgttttg gctgcgttgt
     1561 ttacgatctg gtcgaaagcc aggacggcaa agttccaggt cataaactcc acgatgggac
     1621 gcaggccgtt catggcggct cctacgccga tgccggcaaa gcccaactcc gcaatgggcg
     1681 tatcaatgac gcgcctgggg ccgaactcat cgagcatgcc tttgcttact ttataggcgc
     1741 cgttgtactc ggctacttct tcgcccatca ggaaaaccgt ttcatcgcgg cgcatttcct
     1801 cgatcatggc tt
//
LOCUS       DAORLF010000648         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_33740_length_1811_cov_2.417995, whole genome shotgun sequence.
ACCESSION   DAORLF010000648 DAORLF010000000
VERSION     DAORLF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33740_length_1811_cov_2.417995"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..>1811
                     /locus_tag="PK198_27325"
     CDS             126..>1811
                     /locus_tag="PK198_27325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRF42544.1"
                     /translation="MKNITFAAIALLALASFSCSGPAKQADYDLVIRNALIYDGSGNP
                     PYEGSVAVNADTIAALGDIGAKKGKTEIDAKGMAVSPGFINMLSWATESLLVDGRGMS
                     DLKQGVTLEVMGEGWSMGPLNERMKSEMKKAQGDLKFDIEWTTLGEYLQHLEKKGVSP
                     NVASFMGATTARIHEIGYENRPPTPEELARMQELVRQAMREGALGLGSALIYTPGLYA
                     QQDELIALCQAASEHQGMYVTHMRSEGNRLLESIDEVIDISLKARIPTEIYHLKAAGS
                     KNWPKMKDAAIKLMAARERGIPVSANMYTYTAGATGLDAAMPPWVQEGGYDAWAKRLR
                     DPATRDRVREEMKRDADDWENLYASAGSADNLLLVEFVNDTLKKYTGKTLADVAKLRG
                     ASPEDTAMDLVIQDGSRVGVVYFLMSENNVRSQIALPFMSFGSDAGAPTAEGDFLKSN
                     THPRAYGNFARLLGAYVRDAKIITLEEAVRRLTALPAFNLKIKKRGFLYPGYYADIVI
                     FDPATIADKATYEQPHQYAVGVHHVLVNGVQVLKDGEHTGKFPGRFVKGPGFKE"
BASE COUNT          427 a          583 c          485 g          316 t
ORIGIN      
        1 tgaaggggtg actaccaggc tgaaacttac tgagtcctcc cttcactacc ctaaaaatcg
       61 cgccccgccc catctttccc aacaccgcgc tttttgtatc tttgcccctc tacatcaaag
      121 ctgccatgaa aaacatcacc ttcgccgcca tcgcgctgct cgcgctcgca tcgttcagtt
      181 gttccggtcc tgccaaacag gccgactacg acctcgtcat ccgcaatgcc ctcatttacg
      241 acggcagcgg caatccgccc tacgaaggat cggtcgccgt caatgccgat accatcgccg
      301 cattgggcga tataggcgcc aaaaaaggca aaaccgaaat agacgccaaa ggcatggccg
      361 tctctccggg cttcatcaat atgctgagct gggccaccga aagcctcctc gtggacggcc
      421 gcggcatgag cgacctcaaa cagggcgtca ccctcgaagt gatgggcgaa ggctggagca
      481 tggggccgct caacgagcgc atgaaatccg aaatgaaaaa agcgcagggc gatctcaaat
      541 tcgacatcga gtggacaacg ctgggcgagt atttgcagca cttggagaaa aaaggcgtgt
      601 cgcccaatgt ggcttcgttc atgggcgcca ccacggcccg catccacgaa ataggctacg
      661 aaaaccgccc gcccacgccc gaagaattgg cccgcatgca ggaattggta cgccaggcca
      721 tgcgcgaagg cgcgctcggc ttaggctcgg cgctcatcta cacgccggga ctgtatgccc
      781 aacaggacga actcatcgcg ctttgccagg ccgcgtccga gcatcagggc atgtatgtca
      841 cgcacatgcg cagcgagggc aaccgcctgc tcgaatccat tgacgaagtg atagacatct
      901 cgctcaaagc gcgcattccc acagaaattt accacctcaa agcggccggc tccaaaaact
      961 ggccgaagat gaaagacgcc gccatcaaac tcatggccgc ccgcgaacgg ggcattcccg
     1021 tcagcgccaa tatgtacacc tacacggccg gcgccaccgg attggatgcc gccatgccgc
     1081 cctgggtgca ggagggcggc tacgatgcat gggccaagcg cctccgcgat ccggccaccc
     1141 gcgaccgcgt gcgggaagaa atgaagcgag atgccgatga ctgggaaaat ctctacgctt
     1201 ccgccggctc tgccgacaac ctgctgttgg tggagtttgt caacgatacg ctcaaaaaat
     1261 acacgggcaa aactttagcc gacgtggcca aactgcgcgg cgcatcgccc gaagacaccg
     1321 ccatggacct cgtcattcaa gacggcagcc gggtaggcgt ggtgtatttc cttatgtctg
     1381 aaaacaatgt gcgctcacaa attgccctgc cctttatgag tttcggctcc gatgccggcg
     1441 cacccaccgc cgagggcgat ttcctcaaat cgaacacgca tcccagagct tacggcaatt
     1501 tcgcgcgcct gctgggcgct tacgtccgcg atgcaaaaat catcacgctg gaggaagccg
     1561 tacgccgcct cactgcactg ccggccttca atctcaaaat aaaaaaacgc ggtttcctct
     1621 acccgggcta ctacgccgac atcgtcatct tcgaccccgc caccatcgcc gacaaggcca
     1681 cctacgaaca gccgcaccag tacgcggtgg gcgtacacca tgtgttggtc aacggcgtgc
     1741 aggtactcaa agacggcgaa cacacgggca agtttccggg gcggtttgtc aaagggccgg
     1801 ggttcaagga g
//
LOCUS       DAORLF010000649         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34159_length_1792_cov_1.736327, whole genome shotgun sequence.
ACCESSION   DAORLF010000649 DAORLF010000000
VERSION     DAORLF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34159_length_1792_cov_1.736327"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..209
                     /locus_tag="PK198_27330"
     CDS             33..209
                     /locus_tag="PK198_27330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42545.1"
                     /translation="MSTYDQLIEKGKIEGKIEGKIEVVLNAYANGLSIQLIANITGME
                     EAEVVGILEKHERV"
     assembly_gap    213..310
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..>1211)
                     /locus_tag="PK198_27335"
     CDS             complement(<311..>1211)
                     /locus_tag="PK198_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRF42546.1"
                     /translation="WIIDRNSNTTNVCIANCKFCNFYRRPGHAESYTTTIEEYKEKID
                     ELFLLGGDQLLLQGGHHPDLGLDFYANLFRQLKTLYPDLKLHALGPPEIAHIAKLEKM
                     SHYDVLKVLHESGLDSLPGAGAEILDDRVRRLISKGKCGGQEWLDVMRAAHQLRITTS
                     ATMMFGHIETNMERMEHFVALRQVQSEKPADAKGFIAFIPWPFQDQDTILVKLKRARN
                     TCTSDEYVRMIALSRIMLPNVTNIQASWLTVGKAVAQVCLHAGANDFGSIMIEENVVS
                     AAGARFRFTADGITDQLRNFILT"
     assembly_gap    1212..1277
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1278..1547)
                     /locus_tag="PK198_27340"
     CDS             complement(<1278..1547)
                     /locus_tag="PK198_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42547.1"
                     /translation="MQPATPAYPTAACIRFHPWRNPNQELSALKTKAKLRCYFHPSDK
                     PAAIIFVGQKTKCNTAMRTDDLLHRALQLDFLSAEEGLFLLENATT"
BASE COUNT          374 a          359 c          539 g          356 t
ORIGIN      
        1 ccaaatattc aggacaaaaa aattcaacag ctatgtcaac gtacgatcaa ctcatcgaaa
       61 aaggcaagat cgaaggcaag atcgaaggca agatcgaagt cgtactcaat gcctatgcca
      121 atggtctgag catacagttg attgccaaca tcaccggcat ggaagaagcc gaggtggttg
      181 ggatacttga aaagcacgaa cgggtatagc ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn caggtgagga tgaagtttcg gagctggtcg gtgatgccgt cggcggtgaa
      361 gcggaaacga gcaccggcag ccgacactac attttcctca atcataatac tgccgaaatc
      421 attggccccg gcgtgcagac agacctgcgc cacggctttg cctacggtga gccaggaagc
      481 ctgaatattg gtcacgttgg gcagcatgat gcggctgagg gcaatcatgc gcacatattc
      541 gtcgctggtg caggtgttgc gggcgcgctt cagttttacc aagatggtgt cttggtcctg
      601 aaaaggccag ggaatgaagg caatgaagcc tttggcatcg gcgggctttt cagattgtac
      661 ctgccgcagg gccacgaaat gctccatgcg ctccatgttg gtttcgatgt ggccgaacat
      721 catggtagcg gaggtagtga tgcgcagttg gtgggcggcg cgcatcacat cgagccattc
      781 ctggccgccg catttgcctt tggagatgag gcggcgcacg cggtcgtcga ggatttcggc
      841 gccggcgccg ggcagcgagt cgaggcccga ttcgtggagt actttgagca catcgtagtg
      901 cgacattttt tcgagcttgg caatgtgcgc aatttccggc gggccgaggg catggagctt
      961 gaggtcggga tagagggttt tgagttgccg gaagagattg gcataaaaat cgaggcccag
     1021 atcggggtgg tggccgccct ggaggaggag ttggtcgccg ccgagcagga acagttcgtc
     1081 aattttttct ttgtattcct cgatggtggt ggtgtaagac tcggcatggc cggggcggcg
     1141 atagaagttg cagaacttgc aattggcgat gcacacattg gtggtgttgg aattgcggtc
     1201 tatgatccag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnggt ggtagcgttt tctaataaaa acaggccttc ttcggcactc
     1321 aggaaatcaa gttgtagggc gcggtgcagc aaatcgtctg tcctcatggc ggtattacat
     1381 tttgtttttt gtccaacaaa gataatagcg gcaggtttat cggacggatg aaaatagcag
     1441 cgcagtttag ctttagtctt taaagccgac agctcttggt tggggttgcg ccaggggtgg
     1501 aagcggatgc aggccgccgt tgggtatgca ggtgtggcgg gctgcatgta gcggacgacc
     1561 cgacccgctg cctgtgcggg cgggggctga aggggggctt gcctgtggcg ggtggcgcca
     1621 taaatggaag atcgagggcg gaaattgtgg aaaaaatgag tttctttgca gaacaaaaca
     1681 gcgcacaagt gaccgacaac taccggcata aagggatgcg ccgcaggctg atagagcaac
     1741 tgcggcgcaa gggcataagg gatgaggctg tgctgagcgc gatggagcgg ct
//
LOCUS       DAORLF010000650         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34251_length_1788_cov_1.572418, whole genome shotgun sequence.
ACCESSION   DAORLF010000650 DAORLF010000000
VERSION     DAORLF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34251_length_1788_cov_1.572418"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK198_27345"
     CDS             <1..201
                     /locus_tag="PK198_27345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42548.1"
                     /translation="KDDPVSSAYASHLEDFILEYQPEYWIHGHIHTPAKYEIGKTRII
                     CNPHGHIDEPYNGFEKELIVEI"
     gene            230..>1788
                     /locus_tag="PK198_27350"
     CDS             230..>1788
                     /locus_tag="PK198_27350"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRF42549.1"
                     /translation="MNRPALIILLCATAFLSEAGAQQYIRVNQLGYQPAKTKNAVWCS
                     ITAVEAPAAFSILDANTRKVVWKGKAGKGFGAYGPFAQTCRLDFSGFRQTGRFIIRAG
                     EAESPVFRIAPDAYAQAPDFALRYMRQQRCGYNPFLRDSCHTHDGYTMYGPMPDSTHI
                     DVTGGWHDASDYLQYSTTSANATYHLLAAWRDFPQLFSDAHQAQGTPGSNGIADVLDE
                     ARWGLDWLLKMHPRPDWMFNQIADDRDHVNMRIPKEDPFYGKGFERPVYFCTGEQQGL
                     GKHKNRATGTASTAGKFSSAFALGANMWKQYDPDFAGRLRQHAQSAFDFGLAKPGVCQ
                     TAPNRAPYFYEEDNWKDDMQLAAVALHQSGIAADRDLLQYARQLAADEPVTPWLGADT
                     ALHYQWYPFINIGHYELAKTLRGQQRRAVIEYYRQGIDRVWQKAQANAFYRGVPFIWC
                     SNNLTTSFAIQCYWYRQLSGDRRYEALEQACFDWLFGVNIWGSSFVYGLPADGDTPVD
                     PHSAFTHLKKYP"
BASE COUNT          394 a          564 c          444 g          386 t
ORIGIN      
        1 aaggatgacc cggtaagttc ggcttatgct tctcaccttg aggatttcat tttggaatat
       61 cagccggaat actggattca tgggcatatc cacacgccgg caaaatatga aataggcaaa
      121 acccggatca tctgtaatcc gcatggccat atagatgaac cgtataatgg ttttgaaaaa
      181 gagctgatcg ttgagatttg aaagaagtta ggcatcacga aataacatta tgaaccgtcc
      241 cgccctcatc atcctgctct gcgctactgc ttttttgtct gaagccggcg cgcagcaata
      301 catccgcgtc aaccaattgg gttatcagcc tgccaaaacc aaaaatgccg tatggtgctc
      361 tattacggcc gttgaagcgc ccgccgcctt ttccatcctc gacgcaaata cccgcaaggt
      421 cgtctggaag ggcaaagcag gcaagggttt cggggcttac ggaccatttg cacaaacctg
      481 ccgcctggac ttttccggct ttcgccaaac cggccgattt atcattcgcg ccggcgaggc
      541 agaatcaccc gtttttcgca tcgctcccga cgcgtacgca caagctcccg acttcgccct
      601 gcggtacatg cgccaacagc gctgcggcta caatcccttc ctccgcgatt cctgccatac
      661 tcacgacggc tacaccatgt acggccccat gcctgacagc acgcacattg acgtgaccgg
      721 cggctggcac gatgcctccg attacctcca atattctacc acctcggcca atgcaacgta
      781 ccacctgctg gccgcctggc gcgatttccc gcagcttttc tccgatgcac atcaggcgca
      841 gggcacgccc ggctcaaacg gcattgccga cgtgctcgac gaggcgcgct ggggcttgga
      901 ctggctgctg aaaatgcacc cccgcccgga ttggatgttc aatcagattg ccgacgaccg
      961 cgaccatgtc aacatgcgca ttcccaaaga agaccccttc tacggcaagg gtttcgagcg
     1021 gcctgtctat ttctgcaccg gcgagcagca gggtctcggc aagcacaaaa accgcgctac
     1081 cggtaccgcc tctaccgccg ggaaattcag ttcggctttt gccctgggcg ccaatatgtg
     1141 gaaacaatac gaccccgatt tcgccggccg gttgcgccaa cacgcccaat ctgcctttga
     1201 cttcgggctg gccaaacccg gcgtttgcca gacggccccc aatcgcgcgc cttattttta
     1261 tgaagaagac aactggaaag acgacatgca gttggctgcc gtagccctgc atcagtcggg
     1321 cattgccgcc gaccgcgacc tgttgcaata cgcccggcaa ttggctgcag atgagccggt
     1381 tacgccctgg cttggcgccg atacagccct gcattatcaa tggtatccgt tcatcaatat
     1441 cgggcattat gaattggcca aaaccctgcg cggccaacag cggcgcgccg taattgaata
     1501 ctatcggcag ggcatagacc gggtgtggca aaaggcgcag gccaacgcgt tttatcgcgg
     1561 cgtgcctttt atctggtgct ccaacaacct caccacttcc tttgccatac aatgctattg
     1621 gtaccgccaa ctctccggcg accgccgcta cgaagccctc gaacaggctt gtttcgactg
     1681 gctgttcggc gtcaatatct ggggcagcag cttcgtgtat ggccttccgg cagacggcga
     1741 cacccccgtt gatccccact ctgctttcac gcatctcaaa aaatatcc
//
LOCUS       DAORLF010000651         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34289_length_1786_cov_1.963027, whole genome shotgun sequence.
ACCESSION   DAORLF010000651 DAORLF010000000
VERSION     DAORLF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34289_length_1786_cov_1.963027"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PK198_27355"
     CDS             complement(<1..1530)
                     /locus_tag="PK198_27355"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRF42550.1"
                     /translation="MKRKSTLLVRFAVRGLMMLGFAFAMFTEVQAQALQVNLPGNYSL
                     CSGGSVPLSPQVSGGAGSYTYTWAPATGLSCTTCPNPIASPQQTTDYTLTVRDLENTE
                     ASAMTTVLVSPTATLQVAPAATSVCAGAFVVLTATPSGGSGTCTYRWQIATGPNGPWL
                     DMSSNWNGPELYPATLGWTIGTTHYYRAIYTCSGSLCATATSDPVSVSVAAIPAVSIS
                     PATNFVCQNEPLTLQATVSGGTGNCDIQWLSGPTTSGPWTPVPGAIGATYLPPTDLPG
                     TMYYTASYGCNGTNCGSIIAGQPAKVVTLTPASISVSANDSIVCVGGTPLLTAQLQNG
                     SPECPLQWQRSKFADGPWINISQANQLTYQVPAGNPDTSFYRVVYACLPGPVNTSGIT
                     MTIGSASNVAQGQQVCLDVRVQNFNNIVGMQFSMQYNPAMLHFTSVANFGLPGLTAGG
                     NFGLPLAGAAGITAPGTITLTWTDTDWSGETLPNGTTLFTLCFNALANTSAASVAFTL
                     TP"
BASE COUNT          399 a          463 c          533 g          391 t
ORIGIN      
        1 gggcgtgagg gtaaatgcca ccgaggcagc gctcgtattg gccaacgcat tgaaacacaa
       61 ggtgaacaat gtggttccgt tgggcaacgt ctctccgctc cagtcagtgt ctgtccacgt
      121 cagtgttatg gtgcctggcg cagttatacc cgcagctccg gccagaggca aaccgaagtt
      181 gccgcccgcc gtcaagcccg gtaatccgaa gttggccacc gaagtaaaat gcagcattgc
      241 ggggttgtac tgcatactga attgcatgcc cacaatattg ttgaaattct gcaccctgac
      301 atccagacac acctgctgcc cctgcgccac attgcttgcg ctgcctatcg tcatggtgat
      361 gccggaggta ttcaccgggc cgggcaagca ggcatacacc acccggtaaa agctcgtgtc
      421 ggggttgccg gcgggtacct gataggtcag ttggttggcc tgtgaaatat tgatccaggg
      481 gccgtcggca aatttcgagc gctgccattg cagagggcat tccggcgagc cgttttgcaa
      541 ctgcgccgtc agcagcggcg tgccgcccac acagacgatg ctgtcgttgg cggaaacgct
      601 gatgctcgca ggtgtaagtg taacgacttt ggcgggctgc ccggctatga tactgccgca
      661 gttggtcccg ttgcacccat aagaagctgt gtaatacatc gttcccggca ggtcggtcgg
      721 aggcaggtaa gtggcaccta tggcgccggg tacgggcgtc caggggccgc tcgttgtagg
      781 gccgctcagc cactggatat cgcaattgcc tgtgccgccg gagactgtgg cctgcaaggt
      841 tagcggctcg ttttggcaca caaaattcgt agccggagag atagagaccg cagggatggc
      901 tgctacgctt acggaaacag gatcggaggt agccgttgcg caaagactac ccgaacaagt
      961 ataaatagct cgataataat gcgttgtgcc aatcgtccag cctaaggtag cgggatacag
     1021 ttcggggccg ttccaattgc tggacatgtc caaccacggg ccattggggc cggtagcgat
     1081 ttgccatcgg taggtacaag tgcctgatcc tccgctgggt gtagcagtga gtactacgaa
     1141 tgcacccgca caaacagacg ttgctgccgg agcgacctga agcgtggcag taggagatac
     1201 cagtaccgtc gtcatcgcgc tcgcctccgt attttccaaa tcccgcacgg tgagggtgta
     1261 gtcggtcgtt tgctgaggcg aggctatcgg attggggcag gtagtgcagg aaagcccggt
     1321 cgcaggcgcc caggtgtagg tgtacgaacc cgcgccgccg cttacctgcg ggctgagggg
     1381 gacgctgccg ccggaacaca ggctgtaatt gccgggcaag ttcacttgca atgcctgagc
     1441 ctggacttcg gtaaacatgg caaaggcaaa tcccaacatc atcaggccgc gaacggcgaa
     1501 tcggacaagc agtgttgatt ttcttttcat cgcgcagagt tttttttgac agtacaaaga
     1561 ttggcgcaag tcgcggataa tgcaagcgca attttttggc attgtagatt ggggttgtgc
     1621 ttttttttga atgaattatt gggctgcaag ccgtaagctg cgagccgcaa gcttgatttc
     1681 ccaaaaggaa atgaactcac tttttaaagg cgatttgaga aattccacca agctaaacat
     1741 cttattttta agcaaaaaaa gcctacctgc cggtagtcag gctaaa
//
LOCUS       DAORLF010000652         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34380_length_1782_cov_2.200926, whole genome shotgun sequence.
ACCESSION   DAORLF010000652 DAORLF010000000
VERSION     DAORLF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34380_length_1782_cov_2.200926"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1782)
                     /locus_tag="PK198_27360"
     CDS             complement(<1..>1782)
                     /locus_tag="PK198_27360"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRF42551.1"
                     /translation="QIDNRTTVADFRPEDLQTWSGEQIIAESARYYLASVQHPGLADV
                     AIQQFDAVTTEAKLTDELRPTLFDLLAWRAIEHFSNERGYLTRPAYRFELKEAAAFAD
                     AATFVQHDFATRDTASFHHRAVLLYQQWLKLRLSQNNLSALLDADLHRLRFAYAHSAH
                     PEKEQRYKAALLRAIAARPREAMTADYRHELARWYYQYGSDYKPQTDEDDKKWAWKEA
                     LSICETCIGEHAGAPGVNGCLELKTMILGKELSLQTEQAGVPGQAVLAHISYRNLGRA
                     YLKVVPLKWEERDRFERWETNKDLEWLNKIKPVQQLTVELKNPGDYRQHSTEAALKAL
                     PAGWYAILVSDNDKFRFKDNTAGFVTIQVSNIAYLNRQQPEGGAEFVVVDRYTGAPMP
                     GVKAEFFEENYDWNNRRQIRNRVGEALSNAEGFIASPDAGGRNMSVVFSKGQERLISE
                     GNFSSYRNSWQRPTETVTHFFLDRAIYRPGQTVYFKALVINTNEKRMPSIVADQPVTI
                     TLRDANGQEAAKLELRTNRYGTVNGAFQAPSGGLLGGMSLMSNQGNNSHYFRVEEYKR
                     PRFEVLMQPLEGAAALGEQVTVKGKAQA"
BASE COUNT          309 a          495 c          551 g          427 t
ORIGIN      
        1 gcctgtgctt tgcctttcac cgtcacctgt tcgcccagcg ccgccgcgcc ttctaatggc
       61 tgcatcagca cctcgaagcg gggccgtttg tattcttcta cccggaaata atggctgttg
      121 ttgccctgat tcgacatcag cgacatgccg cccagcaatc cgcccgacgg cgcctgaaat
      181 gctccgttga ctgtgccgta gcggttggtg cggagttcca atttagcggc ttcctgcccg
      241 ttggcgtcgc gcagggtgat ggtcaccggc tgatcggcca cgatggaggg catgcgtttt
      301 tcgttggtat tgatcaccaa tgctttgaaa tacaccgttt ggccggggcg gtaaatggct
      361 cggtccaaaa agaaatgcgt gacggtttcc gtcgggcgct gccaactgtt tcggtaagac
      421 gaaaaattgc cctcggaaat cagacgctcc tgccctttgc tgaacaccac gctcatgttg
      481 cggccgccgg cgtcgggcga tgcgatgaaa ccctctgcat tgctcagcgc ctcgccgact
      541 ctgttgcgta tctgccggcg attgttccaa tcataatttt cttcaaaaaa ctcggccttc
      601 acgcccggca tcggggcgcc ggtgtagcgg tccacgacca caaactcagc gccgccttcg
      661 ggttgctgcc ggttcaggta ggcgatgttc gacacctgaa ttgtgacgaa accggcggtg
      721 ttgtctttaa atctgaactt gtcgttgtcc gacaccaaaa tggcgtacca gccggcgggc
      781 aaggctttca gggccgcctc ggtgctgtgt tggcggtagt cgccgggatt tttcagttct
      841 accgtcagct gttgtacggg ctttatttta ttgagccatt cgaggtcttt gttggtttcc
      901 cagcgctcga aacggtcgcg ttcttcccat ttcaggggca ccactttgag gtaggcgcgg
      961 cccagattcc ggtagctgat atgcgccagc acggcctgtc ccggcacgcc tgcctgttcg
     1021 gtttgcaaag acagttcttt gcccaaaatc atggttttca actccagaca accattcaca
     1081 cccggcgctc cggcatgctc gccgatgcag gtttcacaga tcgagagggc ttctttccat
     1141 gcccattttt tgtcgtcctc atcggtttgg ggcttgtaat cgctgccata ttggtaatac
     1201 caacgggcca actcgtggcg gtagtcggcg gtcatggcct cgcggggacg ggcggcaatg
     1261 gcgcgcagca gggcggcttt gtagcgttgt tctttttcgg ggtgggccga gtgggcgtag
     1321 gcaaagcgca gccggtgcag gtcggcgtcc aacagggccg acaggttgtt ttgcgacaag
     1381 cggagtttca gccattgttg gtacagcagc acggcgcggt ggtggaagga ggccgtgtcg
     1441 cgggtggcga aatcgtgttg tacaaaagta gcggcatcgg caaaagcggc agcctctttc
     1501 agttcgaagc ggtacgccgg tcgggtgagg tagccgcgct cgttggaaaa atgctcgatg
     1561 gcgcgccagg ccagcaggtc gaagagcgtg ggccgcagtt cgtccgtcag tttggcctcg
     1621 gtagtcacgg cgtcgaattg ctggatggcc acgtccgcca gtccggggtg ctgcaccgaa
     1681 gccaagtagt agcgggcgct ttcggcgatg atttgttcgc cgctccaggt ttgaaggtct
     1741 tcggggcgaa aatcggccac cgtagttcgg ttgtcaatct gg
//
LOCUS       DAORLF010000653         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34729_length_1768_cov_1.779918, whole genome shotgun sequence.
ACCESSION   DAORLF010000653 DAORLF010000000
VERSION     DAORLF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34729_length_1768_cov_1.779918"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PK198_27365"
     CDS             <1..922
                     /locus_tag="PK198_27365"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF42552.1"
                     /translation="YNLRYTLPFINRKKTLGIYSYIEYAQNREVNYATEGNKQLFFRL
                     EDRFISQRFRTQLGLNYRPGARTFHYAELGFHQNRIDPIVADSLNPGFFLDGRHLQRY
                     FSFKYEFTRDFRDIRAYPLNGHYLRAGLLKDGLGIFSDRNALTFTIRYERYLPAKGRW
                     SAALMADSKYSLIRQQQPYNDNRALGFGQVYLPGYEYYIADGPDMLLLRSLFRFKLID
                     RTLKFGKWVPLESYRQIPFKAYVAMNAGWAYINDPFASRIGNPLNNRPLWGGGPALHL
                     VFFYDKVLLIEYSFNHLGEKGLFLHLNMNI"
     gene            922..>1768
                     /locus_tag="PK198_27370"
     CDS             922..>1768
                     /locus_tag="PK198_27370"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRF42553.1"
                     /translation="MQVAVFGKVVKEADLPCIQSLFDALHEENINAFVFRAYLDQLKG
                     KIVFRRDVGVFENHRDFIVRPLDYCITLGGDGTILAAIQYTRDNKVPILGINLGRLGF
                     LAGIEKRRISQAIGLLKRGMYEVEHRSLLYLESNLPLFGDTPFALNDCTLLKRDTSSM
                     ITIHAFINGAYLNSYWADGVIVATPTGSTGYSLSCGGPIIFPNSNSFVITPVSPHNLN
                     VRPIVISDDSVISFEVEGRAESFLCTLDSRSESITSAHQLAIRKNDFDIQLIRIPGAG
                     FLETMR"
BASE COUNT          410 a          520 c          410 g          428 t
ORIGIN      
        1 atacaacctc agatacaccc tgcctttcat caacaggaaa aagacgctgg gcatttattc
       61 ctacattgag tatgcccaaa accgcgaggt caactatgcc accgaaggca ataaacagct
      121 ctttttcagg ctcgaagacc gctttatttc tcaaaggttc cgcacccagt tgggactcaa
      181 ctaccgcccc ggcgcacgca cttttcacta tgcagaactg ggttttcacc aaaacagaat
      241 agaccccatt gtagccgaca gcctcaatcc aggcttcttc ctcgacggca ggcacctgca
      301 acgttatttt tcgttcaaat acgaattcac ccgcgatttc cgcgacatcc gcgcataccc
      361 gctcaatggc cactacctcc gggccggcct gctcaaagac ggcctcggca tcttttccga
      421 ccgcaacgcg ctgacattta ccatccggta cgagcgctat ttgccggcca aaggcaggtg
      481 gagcgcagcc cttatggccg acagcaagta ctccctcatc cggcagcaac aaccctacaa
      541 cgacaaccgt gctttgggtt tcggacaagt gtatctgccg ggctacgaat actatatcgc
      601 cgatggtccc gacatgctgc tgcttcgctc gctttttcgt ttcaaactca tagaccgaac
      661 gctgaagttt ggaaaatggg tacctttgga atcttaccgg cagataccct tcaaagctta
      721 tgtggccatg aatgcgggct gggcctacat caatgatccg tttgcaagcc gcatcggcaa
      781 tccgctcaac aacaggccct tgtggggcgg cggaccagct ttgcacctcg tattttttta
      841 cgacaaagtg ctgctgatag aatacagttt caaccatctc ggcgaaaaag gcttattttt
      901 acacctcaac atgaatatct gatgcaagta gcggttttcg gcaaggtagt caaggaagca
      961 gatttgccct gcattcagtc gctgttcgat gccctgcacg aagagaacat caatgctttc
     1021 gtcttccggg cttatttgga tcagctcaaa ggaaaaatcg ttttcaggcg ggatgtgggc
     1081 gtttttgaaa accacagaga tttcattgtc aggccattag actactgcat caccttaggc
     1141 ggcgacggca ctatattggc agctattcaa tacacccgcg acaacaaggt accgattttg
     1201 ggcatcaatc tggggcgcct cggttttctg gcgggcatcg aaaagcggcg catcagccag
     1261 gccatcgggt tgctgaaacg cggtatgtac gaggtagaac accgcagcct cctctatctg
     1321 gagtccaatt tgccgctttt cggcgacacg ccctttgcgc tcaacgattg cacgctcctc
     1381 aagcgcgaca cttcttccat gattaccatc cacgccttta tcaacggcgc gtacctcaat
     1441 tcctattggg ccgacggggt cattgtagcc acgcccaccg gttccacggg gtactcgctc
     1501 agttgcggag gtcccatcat ctttcccaac tccaactcct tcgttatcac gcccgtatcg
     1561 ccgcacaacc tcaacgtgcg gcccattgtc atttcagatg attcggtcat ttcgttcgag
     1621 gtggaaggcc gggccgagag ttttctttgc acgctggatt cgcgttcgga gtccatcact
     1681 tcggcgcatc agttggccat ccgaaaaaac gatttcgaca ttcaactcat ccgaattccc
     1741 ggcgccggat ttttggaaac catgcgcg
//
LOCUS       DAORLF010000654         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_34813_length_1765_cov_2.194152, whole genome shotgun sequence.
ACCESSION   DAORLF010000654 DAORLF010000000
VERSION     DAORLF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34813_length_1765_cov_2.194152"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>237)
                     /locus_tag="PK198_27375"
     CDS             complement(<1..>237)
                     /locus_tag="PK198_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF42554.1"
                     /translation="LLARMVSTYEQLFGDKPHVAACHAGLECGIIGTHYPGMDMISFG
                     PTIRHPHSPDEKVHIASVQKFWKYLLAIVQGTPE"
     assembly_gap    238..368
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<369..1448)
                     /gene="pepD"
                     /locus_tag="PK198_27380"
     CDS             complement(<369..1448)
                     /gene="pepD"
                     /locus_tag="PK198_27380"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HRF42555.1"
                     /translation="MSIRQLEPTALWNHFADLNEVPRPSKHEERVIAFMQNFGRSLGL
                     ETHTDSVGNVIIKKPATPDMEGRKTIVLQGHLDMVHQKNNDTVFDFDAEGIRSYIDGD
                     WVKAQGTTLGADNGIGVASIMTLLASQDIPHPPLEALFTIDEETGMTGAKGLVENLLT
                     GDVLLNLDSEEDNELTIGCAGGLNTNTTIRYAEEAASPGHTGLRISVSGLKGGHSGIE
                     IHLGRGNANKIMNRLLYGMYELCGLRIASVDGGGLRNAIPRESVAMVAVPEDNMQAFQ
                     SEMEQRRQDILREFHSVEPDLRIAAETTAPPQKVMASADQLRLTRAIAAMPNGVYRMS
                     PDIPGLVETSSNLARVEVQGGVFTTL"
     gene            complement(1529..>1765)
                     /locus_tag="PK198_27385"
     CDS             complement(1529..>1765)
                     /locus_tag="PK198_27385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF42556.1"
                     /translation="YCVSKSLLNAMTRHLAYELAGRKIAVNAVCPGWVKTDMGGAGAV
                     RPVEKGAETPVWLAADAEQHLSGKFFRDKREIAW"
BASE COUNT          315 a          451 c          490 g          378 t
ORIGIN      
        1 gctccggcgt gccctgcaca atagccagca ggtatttcca aaacttctgc actgaagcaa
       61 tatgcacctt ctcgtcgggc gagtgcgggt gccgaatggt aggcccaaag gagatcatgt
      121 ccatgccggg ataatgcgtg ccgatgatgc cgcactccaa accggcgtgg caagccgcca
      181 catgaggctt atcgccgaag agctgctcgt aagtactcac catgcgggcc agcagcgnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnca gcgttgtgaa aacgccgccc tgcacttcta cgcgggccag attggaggag
      421 gtttccacta agcctggaat gtcgggactc atgcgatata cgccgttggg catggccgca
      481 atggcgcgcg tgaggcggag ctgatcggcg gaagccatta ctttttgggg tggggcagtt
      541 gtttctgcag cgatgcgaag atcgggttct acgctgtgga attcgcgcag gatatcttgc
      601 cggcgctgct ccatttctga ttggaatgcc tgcatattat cttcgggcac ggccaccatg
      661 gccaccgatt cgcgggggat ggcattgcgc aaaccgccgc cgtcaacaga ggcgatgcgc
      721 aggccgcaga gttcgtacat gccatacagg aggcggttca tgattttatt ggcattgccc
      781 cggccgaggt ggatttcaat gccggagtgg ccgcctttca gtcccgacac actgatgcgc
      841 agacctgtgt ggccagggga agcagcttcc tcggcatagc ggatggtggt attggtattg
      901 aggccgccgg cgcatccgat ggtgagttcg ttgtcttcct cgctgtccaa attgagcaaa
      961 acatcgccgg tcagcaggtt ttccaccaga cctttggcgc cggtcatgcc ggtttcttca
     1021 tcaatggtga aaagcgcttc gagcggcggg tgcggaatgt cttgcgaggc cagcagcgtc
     1081 atgatggaag ccacgccgat gccgttgtcg gcgccgaggg tcgtgccctg cgctttcacc
     1141 cagtcgccat ctatataact gcggatgcct tccgcgtcga agtcgaaaac cgtatcgttg
     1201 tttttctggt gcaccatatc gagatggcct tgcaggacaa tggttttacg gccttccatg
     1261 tcgggcgtgg caggcttctt gatgatgacg ttgcccacgg agtcggtatg cgtttcgagg
     1321 cccagcgagc gcccgaagtt ttgcataaaa gcgatgacgc gttcttcgtg tttggagggg
     1381 cgcggcactt cgttcagatc ggcgaagtgg ttccagagag cggttggttc gagttggcgg
     1441 atggacatgg tatgacgatt atgctgatgt taatgaatga ttcatgttgg cgacaagcgg
     1501 gctatttcct gctcacaaag ataatgattc accacgctat ctcccgctta tccctgaaaa
     1561 acttcccgct cagatgctgc tccgcatcgg cggccagcca caccggcgtt tcggcgcctt
     1621 tttcaaccgg gcgcaccgcc ccggccccgc ccatatcggt ttttacccag cccggacaca
     1681 ccgcgttgac ggcaattttc cgtcctgcca attcgtatgc caggtgcctt gtcatggcat
     1741 tgagcagcga ttttgacacg caata
//
LOCUS       DAORLF010000655         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_35312_length_1745_cov_1.334911, whole genome shotgun sequence.
ACCESSION   DAORLF010000655 DAORLF010000000
VERSION     DAORLF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35312_length_1745_cov_1.334911"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK198_27390"
     CDS             complement(<1..445)
                     /locus_tag="PK198_27390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42557.1"
                     /translation="MTETYQAAAELLPDNATGEAAPRLPGSLWTFTLLGLSLALGITI
                     RGKNPRRLLWLLPLGLLAFVPLNSCKKEAGVTLGFNAEEEVFLQDYYLARRAAGWWCW
                     RPGKPARCGASACIRAGRRCCLAGMSAAAPMACTCGRCRTAQLSAA"
     assembly_gap    453..485
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<486..>1745)
                     /locus_tag="PK198_27395"
     CDS             complement(<486..>1745)
                     /locus_tag="PK198_27395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42558.1"
                     /translation="NNREKAAIELISQLNNKGRALPELERVYQLAPDYDDLRMLMSRE
                     VNKLEDWIFSPRLLRYAGYDYTHGTGEFSEEEGAAARNAEKDALLADLQYLRKVRAFV
                     EKVVKDARQPDQAFWQLTGAYLAFIDGDYEKANALLAAIKNRDKLPINVQLQLGVTEA
                     MVAIMQADAVTPDTDAGLLKLYELIQKNDKAFPEMERFRSEVSLLLSDWLIRKGAKAK
                     GALLLSQCTRSWGEFIGVGRKSMYHKLLDIAGPADYTEALRILSEPATPFEKWLMQNP
                     NHIGKSSDGWGGVYVNGEWLYPGDEKIPAHYYKWNLDRIRDFQSMYYIRRNQIDSALW
                     ALNDIAPAYWKDHPYHEYLKTNPFSAGIQIASLKLTNPQAQVPTDKAAFLRKLIELQA
                     EAERDPKKRSENYLLIANAYYQMSYHG"
BASE COUNT          343 a          452 c          512 g          405 t
ORIGIN      
        1 tggcagcgga tagctgcgcc gtgcggcagc gcccgcaggt acaggccatc ggcgcggcgg
       61 cgctcatccc ggcaagacag cagcgccgtc cagctcgaat gcaggcggat gcgccgcacc
      121 gggccggttt tcccggcctc cagcaccacc agccggctgc gcggcgggcg aggtagtaat
      181 cttggaggaa tacctcttct tcggcgttga atcccaaagt gacgccggcc tcttttttac
      241 aactgttgag cggtacaaaa gcgagcagac ccagcgggag cagccacagc agacggcgcg
      301 gatttttgcc ccggatcgta atacccagcg caagactcag gccgagtaat gtgaaggtcc
      361 aaagactgcc gggcagacgg ggcgcggcct ctccggtggc gttgtcgggc agtaattcgg
      421 cggcggcttg ataggtttct gtcatgcttt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnngcccg tggtagctca tctggtagta ggcgttggcg atgaggaggt agttttcgga
      541 gcgttttttg ggatcgcgtt cggcttcggc ttgtagttca atcagctttc gcagaaacgc
      601 ggctttgtcg gtggggacct gtgcttgcgg attggtgagc tttagggagg cgatctgaat
      661 accggctgaa aaaggattgg ttttcagata ctcgtggtag ggatggtcct tccaataggc
      721 cggggcaata tcattcaatg cccacagcgc actgtcgatc tgattgcggc ggatgtaata
      781 catggactga aaatcgcgga tgcggtcgag gttccatttg taataatgag caggtatttt
      841 ttcatcgccg ggatagagcc attcaccgtt cacataaacg ccgccccagc catcggagct
      901 tttgccgatg tgattggggt tttgcatcag ccatttttca aaaggcgtgg caggctccga
      961 aagtattcgc aacgcctcgg tataatctgc cggaccggca atgtccaata gtttgtggta
     1021 catgctttta cgaccaacgc ctatgaactc gccccatgag cgggtacatt gcgacaacag
     1081 cagcgcgcct ttggcttttg cgccctttcg gatgagccaa tcggaaagca gcagcgaaac
     1141 ctccgaacga aaacgctcca tttcggggaa agccttatcg tttttctgaa tcagttcgta
     1201 caacttcagc aagccggcgt cggtatcggg agtaacggca tcggcctgca taatggccac
     1261 catcgcttcg gtgacgccga gttgtagctg aacgttgatg ggcagtttgt cgcggttttt
     1321 gatagcagca agcagtgcat tggccttttc gtaatcgccg tctataaagg ccagataagc
     1381 gccggtcagt tgccagaaag cctgatcggg ttggcgggcg tctttcacta ccttttctac
     1441 aaaagcgcgc actttgcgca ggtattgcag gtcggccagc agggcatctt tctcagcgtt
     1501 tcgggccgca gcgccttcct cctcgctgaa ctccccggtg ccgtgcgtat aatcatagcc
     1561 ggcgtagcgc aacagccggg gcgaaaaaat ccagtcttcg agcttattca cctcgcggga
     1621 catcagcata cgcaggtcgt cgtagtcggg cgcaagttgg taaacacgct ccaattccgg
     1681 cagtgcgcgg cccttgttgt tgagctggct gatgagttca atggctgctt tttcgcggtt
     1741 gtttt
//
LOCUS       DAORLF010000656         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_36542_length_1695_cov_1.771341, whole genome shotgun sequence.
ACCESSION   DAORLF010000656 DAORLF010000000
VERSION     DAORLF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36542_length_1695_cov_1.771341"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..735)
                     /locus_tag="PK198_27400"
     CDS             complement(250..735)
                     /locus_tag="PK198_27400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42559.1"
                     /translation="MKRLLLTVSVIAVMAAFGSLYGQSYKSAIGARLGVPFAASYKTF
                     ISEPGAIEIFAGFRGYSGYSWVSANAAYQHHFPIGSVENLSWYAGAGAGVQFWNWKNS
                     ALDDSNTSISIMGVIGLDYKFSEIPLNLSVDWMPTYFLGGFIGGFGGGYGSLSARYVL
                     K"
     gene            832..>1265
                     /locus_tag="PK198_27405"
     CDS             832..>1265
                     /locus_tag="PK198_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRF42560.1"
                     /translation="MRKISYQETLDFLFSQLPMFQRIGAPAYKKDLTNITALCEALGH
                     PQRNYPLIHVAGTNGKGSVSFMLSAILQAAGLKTGLCVSPHYRDFRERARINGQYVSK
                     AFVVDFVRRVRPVIDAVQPSFFELSTALAFDYFSSEKVDVAV"
     assembly_gap    1266..1275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1276..>1695
                     /locus_tag="PK198_27410"
     CDS             <1276..>1695
                     /locus_tag="PK198_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF42561.1"
                     /translation="ETGLGGRLDSTNIVEPVLSVITNIGFDHTEFLGNTLPLIAAEKA
                     GIIKAGIPVVIGESHPETAPVFRQTAEHQGSELVFADERYRAARCSGDVLHTVFDIFQ
                     EQKLLYLALEVQLSGDFQTKNIQTVLQAVEILAPQFSI"
BASE COUNT          447 a          428 c          384 g          426 t
ORIGIN      
        1 gcaaacttca gataccggtc accacctgca tgattgcccg cgatccctac ctgatgaatt
       61 ttgtagatca gtttacccgc gccaacaacg gtaaggcttt ttacagcagc ctcaatggcc
      121 tgggcgattt catttttatg gattataaaa acaacaaggc aagaagaaac aggtgatcct
      181 ctaaaaacaa aatggctcca acgcaatgaa gcgctaaagc cattttgatt gcgttatgaa
      241 atttggaatt tacttcagaa cataacgagc tgaaagtgaa ccatatccac ccccaaatcc
      301 gccaatgaag cctcccaaaa agtaggtagg catccagtca acgcttaagt tgagaggaat
      361 ttctgagaac ttgtagtcca atccgataac gcccatgata ctgatgctgg tgttggaatc
      421 atccaatgca gaattcttcc aattccagaa ttgaacaccg gcgccggcgc cggcatacca
      481 agagaggttt tcgacgctgc ctatggggaa atggtgctga taagctgcgt tggcgctcac
      541 ccagctatat ccggagtacc cccgaaatcc agcgaaaatt tcaatagctc ccggctcaga
      601 gataaacgtt ttgtacgatg cggcaaacgg cacgcccaaa cgagcgccta tagcagactt
      661 gtagctctgc ccataaagag aaccgaaggc tgccattaca gcaatgactg agactgttaa
      721 aagtaatctt ttcatgattt tttggttttt gtgatgaaat caaggcacga aattagtcac
      781 cggatttgat ttatatcttc ttttgccttc aattttattt cagatcatca catgcgcaag
      841 atttcctacc aagaaacgct ggatttttta ttcagccaat tacccatgtt tcaacgcatt
      901 ggggcgccgg cctacaaaaa agaccttacc aatatcacag cgctgtgcga agcgctgggg
      961 catccgcagc gcaactaccc actcatccat gtggccggta ccaacggcaa aggctccgta
     1021 tcttttatgt tgagcgctat tctacaggca gccggactga aaaccgggct ttgcgtgtcg
     1081 ccgcactacc gggactttcg ggagcgggcg cgcatcaacg ggcagtacgt ctccaaagcc
     1141 tttgtggtgg attttgttcg tcgcgttcgc cctgtcatag atgcagtgca gccctctttt
     1201 tttgaactct ccacggcgct ggcattcgac tacttttctt ccgaaaaggt ggacgtcgcc
     1261 gtcatnnnnn nnnnngaaac cggcttggga ggcaggctgg attctaccaa tatcgtagag
     1321 cctgtactct cggtcattac caatataggc ttcgatcaca cagagttttt gggaaacacc
     1381 ctgcccttga tcgctgccga aaaagcgggc atcatcaaag ccggcattcc ggtggttata
     1441 ggagaaagtc accccgaaac cgcacctgta ttcaggcaaa ccgctgagca tcagggttct
     1501 gaactcgttt ttgccgatga aaggtatcga gccgctcggt gttcgggcga tgtgctgcac
     1561 actgtttttg atatttttca agaacaaaag ctgctctacc tggcattgga agtacagctc
     1621 tccggtgatt ttcaaacaaa gaatattcaa actgtcttgc aggccgttga aatactggct
     1681 ccgcaattct ccatc
//
LOCUS       DAORLF010000657         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_36687_length_1689_cov_2.224602, whole genome shotgun sequence.
ACCESSION   DAORLF010000657 DAORLF010000000
VERSION     DAORLF010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36687_length_1689_cov_2.224602"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK198_27415"
     CDS             <1..356
                     /locus_tag="PK198_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRF42562.1"
                     /translation="LENWPFDRIMLSYGASLYVRLITQMPVKDPTAGFVCYRRRVLET
                     IDLSKIRFVGYAFQIEMKFATRSLGFSIKEVPITFADRTEGASKMSKGIVKEAIWGVL
                     QMRLRSIGATYALQK"
     gene            353..865
                     /locus_tag="PK198_27420"
     CDS             353..865
                     /locus_tag="PK198_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRF42563.1"
                     /translation="MNTEHHPVPAQTALERRQDALRWVKGASDLMDNRFRIPGTQIRF
                     GLDAIIGLVPYVGDIAGFAMSGVLVLVMARYGASGMLVLRMLGNVALDALVGLIPLLG
                     DIFDITFKANRRNFKLLEAHYEEGKYQGSAWWFVILVLALLVGMFALLLYVMLRLAGA
                     IGLWMWGLFS"
     gene            1097..>1304
                     /locus_tag="PK198_27425"
     CDS             1097..>1304
                     /locus_tag="PK198_27425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42564.1"
                     /translation="MKRIMLLVILGLVSLAACRQAEKAPDTATPATETPEVSETDFNW
                     NPENFGDKRILRYQIPGFDNLSVQQ"
     assembly_gap    1305..1317
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1318..>1689
                     /locus_tag="PK198_27430"
     CDS             <1318..>1689
                     /locus_tag="PK198_27430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRF42565.1"
                     /translation="YLVQAGLAGRDIIWDQNYRHNLAVRKALETAIKNYKGDQQAAEW
                     QQFMEYAKNVFFSNGIHHHYSNDKFIPAFPRPWFESVLKDVGHSLPEEVLTAMFDPKV
                     DAKKINQDPKVDVITASAVNF"
BASE COUNT          376 a          425 c          496 g          379 t
ORIGIN      
        1 agttagaaaa ctggcctttc gatcgcatta tgctctcgta tggcgcttcg ctgtatgtgc
       61 ggctcatcac gcagatgccg gtgaaagacc ccaccgcggg gtttgtgtgt tatcgccggc
      121 gggtactgga aaccatagac ctgagcaaaa tccgttttgt gggatacgcc ttccagatag
      181 agatgaaatt tgcgacccgt tcgctcgggt tctctattaa agaagtaccc attacgtttg
      241 ccgaccggac cgagggcgcg tccaaaatgt cgaaaggcat tgtaaaagaa gccatttggg
      301 gcgtgctgca aatgcggctc cgtagtattg gtgcaaccta tgctttgcaa aaatgaatac
      361 tgaacatcat cccgtgcctg cacaaacagc gctcgaacgg cggcaggacg ccctccgctg
      421 ggtaaaaggc gcgagcgacc tgatggacaa ccgctttcgc attccgggca cacagattcg
      481 ctttggcttg gacgccatta tcgggctggt gccttatgtg ggcgatatag ccggctttgc
      541 catgtcgggc gtgttggtat tggtgatggc gcggtacggc gcgagcggca tgttggtgct
      601 gcgtatgctg ggcaatgtgg cattggatgc gctggtagga ttgatacctt tactgggaga
      661 tattttcgat ataaccttca aggccaaccg gcgcaatttc aagctgttgg aagcgcacta
      721 tgaggaaggc aagtatcagg gcagcgcctg gtggtttgtg atattggtgc tggcgctgct
      781 ggtgggtatg ttcgccctgc tgctgtatgt gatgctcaga ttggcaggcg ccatcggctt
      841 gtggatgtgg ggtttgttta gttgaatgtc gcgcttgcgg caggggcgga agtggatggc
      901 ccgcagccgg gaagactttg gaaagcgggc tgagggccat agaccggacg acccctgact
      961 gccgtcaggc aggcggcgcc ggagtttgac tgctgccggc agtgggttgt aataggaggc
     1021 cggcagcaca ggtggaccgg cggccgccac catccctttc ccgttttcga tcagcaataa
     1081 ccaaatacgc aataccatga aaagaatcat gctcttggtc atcttggggc tggtgtcgct
     1141 ggcagcttgc cggcaggccg aaaaagcccc cgatactgcc actcctgcta cagaaacgcc
     1201 ggaggtatcg gaaacggatt tcaactggaa tcctgaaaac tttggcgaca aacgcatact
     1261 acgctaccag attccgggtt tcgacaacct gagcgtacag cagannnnnn nnnnnnnact
     1321 acctcgtgca ggccggtttg gctggtcgcg acatcatctg ggatcagaat taccgccaca
     1381 acttagccgt gcgcaaggcg ctggaaactg cgatcaaaaa ctacaaagga gaccagcaag
     1441 ccgctgaatg gcagcagttt atggaatatg ccaaaaatgt gtttttctcc aacggcattc
     1501 accaccacta ctccaacgac aagtttattc cggccttccc gcgcccctgg ttcgagtcgg
     1561 tgctcaagga cgtaggccac agtctgccgg aggaagtgct gacggccatg tttgacccga
     1621 aagtggacgc caaaaaaatc aatcaggacc ccaaggtgga cgtgattacc gcttctgccg
     1681 tcaattttt
//
LOCUS       DAORLF010000658         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_36715_length_1688_cov_1.741580, whole genome shotgun sequence.
ACCESSION   DAORLF010000658 DAORLF010000000
VERSION     DAORLF010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36715_length_1688_cov_1.741580"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK198_27435"
     CDS             complement(<1..344)
                     /locus_tag="PK198_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRF42566.1"
                     /translation="MATTTASTTHRLRRVFVWELPVRVYHWLNALTIIVLCVTGYFIG
                     NPPALMSQTEASFRYLFGTIRFLHFAAAYLFFFNFAFRIYWGFVGNKYADWKNFIPTN
                     KKYFKEIWEVLK"
     gene            complement(358..>579)
                     /locus_tag="PK198_27440"
     CDS             complement(358..>579)
                     /locus_tag="PK198_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF42567.1"
                     /translation="NYQMVVPTTWNASPRDPEGKMSAYESSLMGTPIADPEQPVEILR
                     TVHSFDPCLACAVHLYDEDGKHISKVKVL"
     assembly_gap    580..613
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<614..>948)
                     /locus_tag="PK198_27445"
     CDS             complement(<614..>948)
                     /locus_tag="PK198_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF42568.1"
                     /translation="KGHAMEVGPLARVLVGYARGREDFKEVVDSTLAKLNVPAAALFS
                     TLGRTAARGLESLLVAQWGIEFFDQLIANIKNGDRRMADTTMFDPKTWPKQAHGVGMM
                     EAPRGALAH"
     assembly_gap    949..973
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<974..>1332)
                     /locus_tag="PK198_27450"
     CDS             complement(<974..>1332)
                     /locus_tag="PK198_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF42569.1"
                     /translation="VEQVYIPDLLAVAPYYLDWGGIGGGLGNFMAYGDMPTNGYNDRN
                     NLKFPSGVILNSDLSKIHEVDPTDDAEIREFIERSWYEYSVGDKEGLHPWKGETDIKY
                     TGPKPPYDYLDVEKKYS"
     assembly_gap    1333..1355
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1356..>1688)
                     /locus_tag="PK198_27455"
     CDS             complement(<1356..>1688)
                     /locus_tag="PK198_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRF42570.1"
                     /translation="SISHWPKSSPGYFSDLQKRLGAFVESGQLGIFANGYWGHPAYKL
                     PAEINLIGVAHYLEALEWQKEIVKVHTIFGGKNPHPNYLVGGMACSIGIDDASAINAE
                     RLALVEKLL"
BASE COUNT          351 a          443 c          434 g          378 t
ORIGIN      
        1 attttcaaca cctcccatat ttccttaaaa tacttcttgt tggtaggaat gaagttcttc
       61 cagtcggcat atttgttgcc cacaaaaccc cagtaaatcc tgaacgcgaa gttgaaaaag
      121 aaaagatatg ctgccgcaaa atgcagaaaa cgtatggtgc cgaacagata gcggaacgaa
      181 gcctcggttt gagacatcag ggccggcggg ttgccgatga aatagcccgt cacgcacaac
      241 actatgatgg tgagcgcatt gagccagtga tatacccgca cagggagttc ccacacgaaa
      301 acccggcgaa gcctatgagt agtggatgca gttgttgttg ccatgattat caatcattta
      361 caatactttc actttgctga tgtgcttgcc gtcttcatcg tacaggtgta cggcgcaggc
      421 cagacacggg tcgaaggagt gtacggtacg caaaatttcc accggctgtt ccgggtcggc
      481 aatgggcgtt cccatcagcg aagattcgta agccgacatc ttgccttcgg ggtcgcgggg
      541 cgacgcgttc caggtagtag gcactaccat ttggtaattn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnngtgggcc aacgcgccgc gcggcgcttc catcatgccc acgccatgcg
      661 cttgtttcgg ccaggttttg ggatcgaaca tggtggtatc ggccatgcgg cggtcgccgt
      721 ttttgatgtt ggcaatcaat tgatcgaaaa actcgatgcc ccactgcgcc accaaaaggc
      781 tctccaaacc gcgagccgcc gtgcgcccca gcgttgaaaa cagcgcggca gccggcacat
      841 tgagcttggc caaagtgctg tctaccactt ctttaaagtc ttcccggcca cgggcgtacc
      901 ctaccagcac gcgagccagc ggaccaacct ccatggcgtg tcctttccnn nnnnnnnnnn
      961 nnnnnnnnnn nnngctgtat tttttctcca catccaaata gtcatacggc ggtttcgggc
     1021 cggtgtattt gatgtcggtt tcgcctttcc aggggtgtag cccttccttg tcgcctaccg
     1081 aatactcgta ccaggagcgc tcgatgaact cccggatttc ggcgtcgtcg gtgggatcca
     1141 cctcgtgtat tttgctcaga tcgctgttga gaatcacgcc cgacgggaat ttgagattgt
     1201 tgcgatcgtt gtagccgttg gtgggcatat cgccataagc catgaaattg cccaagccgc
     1261 cgccaatgcc gccccagtcc aaatagtacg gtgcaacagc cagtaaatca ggaatataca
     1321 cctgctctac gannnnnnnn nnnnnnnnnn nnnnnagtag tttttccacc aaagccagac
     1381 gttcggcgtt gatggcgctg gcgtcgtcta tgccgatgga gcaggccata ccgcccacca
     1441 agtagttggg gtgcgggttc ttgccgccga agatggtgtg taccttcaca atctccttct
     1501 gccattccag cgcctccaga tagtgcgcta ccccgatgag gttgatttcg gcaggcagtt
     1561 tataggccgg gtgcccccag tatccattgg cgaaaatgcc aagctgaccg ctttccacaa
     1621 aggcgccgag gcgtttttgc aggtcggaga agtagccggg cgagcttttg ggccagtggc
     1681 tgatgctc
//
LOCUS       DAORLF010000659         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_37727_length_1649_cov_1.945420, whole genome shotgun sequence.
ACCESSION   DAORLF010000659 DAORLF010000000
VERSION     DAORLF010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37727_length_1649_cov_1.945420"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PK198_27460"
     CDS             <1..844
                     /locus_tag="PK198_27460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRF42571.1"
                     /translation="ALLEKMQSEAAQPQADIFIASDAWYLEQARKAGLLQPFSTEKMD
                     RNVPTRYRDNAGHWTALDRGAVGLAYAEGRVDLRKLYVYNDITSPAYRGKLLLGNPGL
                     AANRGLVAGLIALNGEAATEKWLQAIIANRATGVEAANNFDLIKALAAGKADMAVVPS
                     NVLLQMKASGNPEYTTPAAQVGFIYLTQPDKTSVFYTSGAGLLKNAPHRDFAVMLLEF
                     LTAAEQQPALVGATIDLPINPMSLPHDLFDEIGGFYEIKKSLNEVCDQYEAADALMQK
                     TGWK"
     gene            855..1637
                     /locus_tag="PK198_27465"
     CDS             855..1637
                     /locus_tag="PK198_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF42572.1"
                     /translation="MQYQHLLLEEKDNILIVTINREKALNALNTATMLELQHLFGSDA
                     PQRSHIAGLILTGAGERSFVAGADISEFLGMDAARGAEMARRGQDIFFLIERFPKPVI
                     AAVNGFALGGGCELAMACHLRIAGEKARFGQPEVNLGLIPGYGGTQRLVQYVGKTKAL
                     ELLMTADMIDAQEAHRLGLVNAVVPAGEEVKAAMAMLQKIAAKAPLAIAGVIASVNAY
                     YQHDEDGFDREVREFGLTAGTEDFREGASAFLEKRKAAFTGR"
BASE COUNT          389 a          442 c          467 g          351 t
ORIGIN      
        1 agccctgttg gaaaaaatgc aatcggaggc cgcacaaccg caggccgaca tcttcattgc
       61 ttccgatgcc tggtacctcg aacaagctcg gaaggcgggc ctgttacaac ctttttctac
      121 cgaaaaaatg gacagaaatg tgcctacccg ctatcgggac aacgccgggc attggacggc
      181 cttagaccgg ggcgccgtgg ggctggctta cgccgagggg cgcgtggacc tgcgcaaatt
      241 atatgtttac aacgacatca cttccccggc atatcgcggg aaattgttgc tgggcaatcc
      301 gggactggcg gccaatcgcg gtctggtagc aggcttgatt gccctgaacg gtgaagccgc
      361 tacggaaaaa tggctacagg ccatcatcgc caacagagca acgggcgtgg aagctgccaa
      421 caatttcgat ctcatcaaag cgctggctgc cggcaaagcc gatatggccg tcgtgccctc
      481 caatgtgctg ctgcaaatga aggcaagcgg caatcccgaa tacaccacgc cggcggcgca
      541 ggtagggttc atttacctca cgcaacccga taaaacctct gtcttttaca catcgggggc
      601 aggtttgttg aaaaatgccc cgcatcggga tttcgcagtt atgttgctgg aatttctcac
      661 cgcggccgag cagcaaccgg ctttagtggg cgctaccatt gatttgccca tcaatcccat
      721 gtcgctgccg catgacctgt ttgatgagat cggcggtttt tacgagatca aaaaatcgct
      781 taacgaagtt tgtgatcaat acgaagccgc cgacgcgctt atgcagaaaa cgggctggaa
      841 ataaagtact tatcatgcaa taccaacatc tccttctgga agaaaaagac aacatcctga
      901 tcgttacgat caatcgggaa aaagccctca acgcgctcaa cacggctacc atgctggagt
      961 tgcagcattt gtttggttcc gatgcgccac agcgttcgca catcgcgggc ctgatcctca
     1021 ccggtgcggg cgagcgctct tttgtggccg gcgccgacat cagcgagttc ctgggtatgg
     1081 atgctgctcg cggtgcggaa atggcccgtc gcggacagga tatattcttc ctcatcgagc
     1141 gatttccaaa gcccgtcatc gccgccgtca atggttttgc gctgggcggc ggctgcgaat
     1201 tggcgatggc ctgtcatctg cgcatagcgg gcgaaaaagc gcgtttcggg cagccggaag
     1261 tcaatctggg cctcattccg ggctatggcg gcacgcagcg tttggtacaa tacgttggta
     1321 aaaccaaagc gctggaactg ctgatgaccg ccgatatgat agacgcgcag gaagcccatc
     1381 ggctgggttt ggtcaatgcc gttgttccgg ccggcgagga ggtaaaggct gctatggcca
     1441 tgttgcagaa aattgcagcc aaagccccct tggccattgc cggagtcatt gcctcggtaa
     1501 atgcctatta ccaacacgac gaagacggct ttgaccgcga agtcagagaa tttggtctaa
     1561 cggcaggcac agaagatttc cgggaaggag cctcggcatt tctggaaaag cgaaaagcag
     1621 cttttacagg tcgttgataa ctaaatgtg
//
LOCUS       DAORLF010000660         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_37761_length_1647_cov_2.857412, whole genome shotgun sequence.
ACCESSION   DAORLF010000660 DAORLF010000000
VERSION     DAORLF010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37761_length_1647_cov_2.857412"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..692)
                     /locus_tag="PK198_27470"
     CDS             complement(33..692)
                     /locus_tag="PK198_27470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRF42573.1"
                     /translation="MPHLSPTLYADLPQVARCHCAAFPSSLATALGRKYAAHMLSWYL
                     SSDKTFIFHVVGERGEVAGYCGGMVSDGTLGTGSASGMAQHSFRAAMWAFATHPWVLW
                     HPEVRAKWPLLWKNLMMKLGLRSKVHFSAAEKTARAQAPQVGLVVIGVDPKYQGQGLG
                     SVLLKEFERVATEVYGIGNLQLSVLADNEQAIRAYERNGWVREREQGRSLSMRKLVER
                     E"
     gene            complement(830..1273)
                     /locus_tag="PK198_27475"
     CDS             complement(830..1273)
                     /locus_tag="PK198_27475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF42574.1"
                     /translation="MPTNLYGPNDNYHPENSHVLPALLRKFHEAKLAEQPFVTMWGTG
                     KPLREFLHVDDLADACVFLMRHYDEAGHVNVGTGEDISILELAQMVQRIVGYEGEIRH
                     DLDKPDGTPRKLLDVSKLQALGWQARIPLEQGVQQVYEEYRMLSQ"
     gene            complement(1230..>1647)
                     /locus_tag="PK198_27480"
     CDS             complement(1230..>1647)
                     /locus_tag="PK198_27480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF42575.1"
                     /translation="SSSELDLRNQEQVRRFFDKERPAYVFLAAAKVGGILANNTYRAE
                     FLYDNLMIESNIIHAAQESGVEKLLFLGSSCIYPKMSPQPIREEYLLTGLLEPTNEPY
                     AIAKIAGIKMCDAYRAQPVHIGHAHQFIRSQRQLPS"
BASE COUNT          345 a          543 c          408 g          351 t
ORIGIN      
        1 cgttcattcg cgttaaaact tcgcgttcca cgtcattcgc gttccacgag ttttcgcata
       61 ctcaaactcc gcccttgctc ccgctcccgc acccaaccgt tgcgctcgta cgcccggatg
      121 gcctgctcat tatcggccag cacgctgagt tgcaaattac cgatgccata tacctccgtt
      181 gccacgcgct cgaactcttt caataaaaca ctccccaagc cctgcccctg atacttcgga
      241 tccacaccaa tgaccaccaa gcctacctgc ggcgcctgcg cccgcgctgt cttttccgca
      301 gcactgaaat gtactttgga gcgcaagccc aatttcatca tcaggttttt ccacaacaaa
      361 ggccacttgg cccgcacttc gggatgccac aatacccagg gatgcgtggc aaatgcccac
      421 atcgccgccc ggaaactgtg ctgcgccata ccgctggccg aaccggtacc taatgtgccg
      481 tcgctcacca tgccgccgca atagccggct acttcgccgc gctcgccgac gacatgaaat
      541 ataaaggtct tgtcgctcga caaataccac gacaacatgt gcgctgcata cttccgcccc
      601 agtgccgtag ccaaagagct gggaaaagcc gcacagtgac aacgcgctac ctgcggtaaa
      661 tcagcgtata gggtggggga gagatgaggc atgacgaaat tttcgggttg gaacgggctt
      721 cggttcaatg cttctggttg gaacggctgc tggctataag ctgcaaccgc gccatggcgc
      781 ggtgctggcc gcgtttcgct cgcgctgagc accgcccacc gcctactgcc tactgactca
      841 gcattcgata ttcctcatac acctgctgca ctccctgctc cagcggaatc ctcgcctgcc
      901 agcccagcgc ctgcaatttc gatacatcca gcagctttct cggcgtgccg tcgggtttgt
      961 ccagatcatg gcggatttcc ccctcgtagc ccacaatgcg ctgcaccatt tgcgccagtt
     1021 ccagaatcga aatgtcttcg cccgtgccca cgttcacatg cccggcctcg tcgtagtgcc
     1081 gcatcagaaa cacgcaggca tcggccagat catccacatg caaaaactcc cgcaagggct
     1141 tgcccgtgcc ccacatcgtg acaaagggct gctcggccag cttggcctcg tgaaacttgc
     1201 gcaacagcgc cggcaataca tggctgttct caggatggta gttgtcgttg ggaccgtata
     1261 aattggtggg catggccgat atgaaccggc tgcgcccggt acgcgtcgca catcttgatg
     1321 cccgctattt tggcaatggc atacggctcg ttggtcggct ccagcaggcc cgtcagcaga
     1381 tactcctcgc ggatgggctg cggcgacatc ttgggataaa tgcacgagga ccccagaaac
     1441 aacaatttct ccacgccgct ctcctgcgcc gcatggatga tgttgctttc tatcatcagg
     1501 ttgtcgtaca aaaactcggc gcgataggtg ttgttggcca atataccgcc caccttggcc
     1561 gccgccagaa agacatacgc cggacgctct ttgtcaaaaa aacgccgcac ctgctcctgg
     1621 ttgcgcaaat ccaattcgga ggaactc
//
LOCUS       DAORLF010000661         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_38135_length_1633_cov_1.828264, whole genome shotgun sequence.
ACCESSION   DAORLF010000661 DAORLF010000000
VERSION     DAORLF010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38135_length_1633_cov_1.828264"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="nadC"
                     /locus_tag="PK198_27485"
     CDS             <1..720
                     /gene="nadC"
                     /locus_tag="PK198_27485"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRF42576.1"
                     /translation="GVLAGVEVARRTFELVAPDAVFVQHIEDGADVSVGEKAFEVQCN
                     TRTLLGAERVILNTMQRMSGIATLSNRFHFEVEDLPVTILDTRKTTPLIRFLEKWAVR
                     LGGCTNYRDGLYDRFLIKDNHVEACGSVQAAIEGVLAYRKANDLEHLGITIEVRNLVE
                     LYAALEVGHIDRIMLDNFELPLLQEAVDIVAGRYETEASGGVNIHNVRKIALTGVNFI
                     SVGALTHSAVGLDLSLKIRGH"
     gene            760..1437
                     /locus_tag="PK198_27490"
     CDS             760..1437
                     /locus_tag="PK198_27490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42577.1"
                     /translation="MFLVPAFAQTKAQTIVTDSVLYQQLTNNLKTINDSLDFYVTDEF
                     ESVWPETPIVATLAQFMKDQPFVIFIGVHTACREGQRSSSEIAQKIADNLQKSLIYNG
                     IERQRLKAYGLEPCLPFHQIQEESRPVYSSNRVTIKMIGWSDQPVENQGLSVDDCIGK
                     VVLEICVNAKGSVLYVKPDLEKSTSTEKSCIDNAVANAYNWKFGSAATGLQCGSMTYD
                     FQNAARR"
BASE COUNT          415 a          389 c          422 g          407 t
ORIGIN      
        1 ggcgttctgg ccggtgttga agtggcccgc cgcacctttg aattggtggc gcccgatgca
       61 gtttttgtgc aacatattga agacggcgcc gatgtcagtg ttggtgaaaa agcctttgag
      121 gtgcaatgca atacccgcac gctgctgggc gccgagcgcg tgatcctcaa taccatgcag
      181 cgcatgagcg gcattgccac cctgagcaac cgctttcatt ttgaggtgga agacctgcct
      241 gttaccatcc ttgatacgcg taaaaccacg ccgctcattc gctttcttga aaaatgggcg
      301 gtgcgattgg gtggatgtac caactaccgc gacggtctct atgatcgttt tcttatcaaa
      361 gacaatcatg tcgaagcctg cggcagcgtg caggctgcca tagagggcgt gctggcgtac
      421 agaaaagcca atgatctgga acatctcggc atcaccattg aagtgcgcaa tctggtagag
      481 ctctacgccg ctctcgaagt ggggcatatt gatcgcatca tgctcgataa cttcgaactg
      541 cctctgttgc aggaagcggt ggacatcgtg gccggtcgct acgaaacgga ggcctccggc
      601 ggcgtcaata tccacaacgt gaggaagata gcgctcaccg gcgtcaattt tatttccgtg
      661 ggcgcgctga cacactcggc ggttggtttg gatttgagtt tgaagatcag ggggcattag
      721 gagggctgct gttctatgaa gacactcttc atttcatggt tgtttttagt gccggctttt
      781 gcacagacca aggctcagac tattgtgacg gacagtgttt tataccaaca attgaccaat
      841 aatctgaaaa cgatcaacga cagcctggac ttttatgtga cggatgaatt cgagtcggtc
      901 tggcctgaaa caccaatagt ggcaacgctg gcgcagttta tgaaagacca gccttttgtc
      961 atcttcatag gcgtacatac agcttgccgg gaaggtcaac ggtcgtcgtc ggaaatcgct
     1021 caaaaaatag ccgacaatct ccaaaaatca ctgatttaca atggcattga gcgacaaagg
     1081 ctaaaggctt acgggctgga gccttgcctg ccgtttcatc aaatccaaga ggagagcagg
     1141 cctgtttatt cgtccaatcg agtgaccata aaaatgatcg gctggtcgga ccaacctgtt
     1201 gaaaatcagg ggttatctgt tgacgactgc atcggcaagg ttgttcttga gatttgtgta
     1261 aacgcaaaag gatcagttct ttatgtgaag cccgatctcg aaaaatcaac ttcaacagaa
     1321 aaaagctgta ttgacaatgc tgttgctaat gcttacaatt ggaaattcgg ctctgctgcg
     1381 accggtctgc aatgtggcag tatgacttac gactttcaaa atgctgcccg tcggtaaagt
     1441 tcaggtatag cgataaagtt acaaaccact catttatgaa atatctccac ttgtctcttc
     1501 ttctccttct gcttgcgttt tcctgcgaaa aaaaggaacc cgatgagccg atgacgcccg
     1561 atttggagga gttcaacatg ctgatgggca accccagcca ggctaccaaa gagacgagca
     1621 atccgaacaa tta
//
LOCUS       DAORLF010000662         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_38316_length_1627_cov_1.804071, whole genome shotgun sequence.
ACCESSION   DAORLF010000662 DAORLF010000000
VERSION     DAORLF010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38316_length_1627_cov_1.804071"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    177..195
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<196..634)
                     /locus_tag="PK198_27495"
     CDS             complement(<196..634)
                     /locus_tag="PK198_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42578.1"
                     /translation="MKRLLPFLALGILVIGAGSCGQDRGVASSTSSYDYTIVPGERVG
                     LITLEKCSREEVLAAYGDSARVEEIYLVEGMTGEGVVIFPDNPRNRMEVYWDSEIDPV
                     RPVLIRISGEGTDWKTTQGITVGTPIAEVEKANGKPFLLSGFNW"
     gene            complement(705..>1627)
                     /locus_tag="PK198_27500"
     CDS             complement(705..>1627)
                     /locus_tag="PK198_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF42579.1"
                     /translation="TAVVVFYTYIGGMWAVSITDFIQTIIIIIGLVILAVYIGHAAGG
                     FSKVIASTPEGFFRFLPEGNFNSIAHYFAAWITIGLGSIPQQDVFQRVMSAKDAKTSV
                     RASYIGGFMYLTIGFIPLFIALAGRTLYSDMAEGDPQMMVPMMVLRHAPMALQVLFFG
                     ALLSAILSTTSGAMLAPATIIGENLVRPYFKNVSDQKLLTIMRMSVVFVAVLSAVMAS
                     WSADIFELVGQSSAFSLVSLFFPMTLGMYWKRSSTVGALASMIAGLAVWLFFEIRGSE
                     LPSLLHGALVGLVVMVAGSLIWPDKGHRVG"
BASE COUNT          424 a          501 c          374 g          309 t
ORIGIN      
        1 cggggaaaac tcagttccag ccccgttacc accacgcctg ccggtgtcaa agcaggcata
       61 tcactgctca attccacatc tccggaaata ccttcaggcg tctctccttc agcattatag
      121 ccaaattgaa gaccgagatt gttgtttagt tttccttgat tccagttggt cgtccgnnnn
      181 nnnnnnnnnn nnnnncccag ttgaacccgg agagcaggaa gggcttgccg ttggcttttt
      241 ctacctccgc aatgggtgtg cccaccgtga tgccctgcgt ggttttccag tcggttcctt
      301 caccgctgat gcgaatgaga acagggcgca ccgggtcaat ttccgaatcc caatacacct
      361 ccattctgtt gcgcgggtta tcgggaaaaa tcactacgcc ctcgccggtc atgccttcta
      421 ccaagtaaat ttcctccacc cgggcgctgt cgccgtaagc ggccagcacc tcctcccgac
      481 tgcatttttc cagagtaatc agacccactc gctcgccggg cacgatggta taatcgtaag
      541 aggatgtgga agacgcaact cccctgtcct gaccgcagga accggcgccg atcacaagta
      601 tgcccagcgc taaaaacggc aacaatcttt tcattatcaa taggttttaa agtgaacgta
      661 ccaacagata aactgccgaa gccgcgcaag gcgcgggaag cagcttaccc tacgcgatgc
      721 cccttatccg gccagatcaa actccccgcc accatcacca ccaatcctac caaagcccca
      781 tgcaacaaac tcggcaactc gctgccccgt atctcaaaaa acagccacac cgccaggccc
      841 gcaatcatgg aggccagcgc gcccaccgta gaggagcgtt tccagtacat gcccagggtc
      901 atcggaaaaa acagcgacac taagctgaac gccgacgact gccccaccaa ctcaaaaata
      961 tcagccgacc aactcgccat caccgccgaa agcaccgcca caaacaccac ggacatccgc
     1021 atgatggtca gcagtttttg gtcgctcacg tttttgaaat acggccgcac taggttctcc
     1081 ccgatgatcg tggcaggagc cagcatcgcg ccggaagtgg tactcaaaat ggctgataac
     1141 aaagccccga aaaacaacac ctgcaaggcc ataggcgcat ggcgcaatac catcatgggc
     1201 accatcatct gagggtcgcc ctctgccatg tcgctgtaca aagtacggcc cgccaacgct
     1261 atgaacaaag gtataaaacc aatggtgagg tacatgaaac cgccgatgta agaggcccgg
     1321 acggaggttt tggcatcttt ggccgacatg acccgctgaa acacatcctg ctgcggaata
     1381 gaccccaaac cgatggtgat ccaggccgca aaataatggg caatgctgtt gaaattgcct
     1441 tccggcagaa atcggaaaaa gccctccggc gtggaagcaa tcactttgga aaagccgccc
     1501 gccgcatgtc ctatgtaaac cgccagtatc accagaccga tgatgatgat gatggtttga
     1561 ataaaatcgg tgatggacac cgcccacatg cccccgatat aggtataaaa aaccaccacc
     1621 gccgtgc
//
LOCUS       DAORLF010000663         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_38948_length_1606_cov_1.897485, whole genome shotgun sequence.
ACCESSION   DAORLF010000663 DAORLF010000000
VERSION     DAORLF010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38948_length_1606_cov_1.897485"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    255..399
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <400..>1606
                     /locus_tag="PK198_27505"
     CDS             <400..>1606
                     /locus_tag="PK198_27505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF42580.1"
                     /translation="PGVSFSPDRKWMMLMDQPGNPSIEELSQPELRLAGIRINPRTNG
                     PSRSNYYTNLTIKNIANGAEKKVQRLPAKARIQYVSWSPDGSKAAFVNAVEAGLELWV
                     VDVEAATARIFSSPAVNAAMPGSPYSWIGNDQILISMIPTGRGPAPQAPLTPAGPIVQ
                     ANEGKAAPVRTFQDLLTNAHDEALFEHYGASQLMLVDLKQGTRTGMGEAGIITDFNVS
                     PDGKYLLVTALQKPFSYIVPYSRFPQTTAIWNMQGQVLKVIVQKPLMDKLPQGFDAVA
                     TGPRSISWRADAPATLYWVEAQDGGDPKKEVAVRDKMYALEAPFGEQPKEVISIALRY
                     RGISWGKGDLALAYEGWWQNRRQVTSIWQPDNTAAGKTSLFERSTEDRYNDPGGFVTQ
                     LNEYGRSVLL"
BASE COUNT          345 a          409 c          386 g          321 t
ORIGIN      
        1 aacggctgct ttttttattc ttctgctcat aatgatgcgg atattcgatg gttgaagatg
       61 agttggcgct gcgaaagtaa ggcgtttttt tctaattcag cgaaaattcg ccaaatgtaa
      121 gttctaaact tctgaaatga aaagccggtt caccaatctg ccttgtttgc ggatttcaaa
      181 aacaactatt acatttgtca gcctcccaat tagggaaggc tttgtccaat tgtttgaaac
      241 cagatcaaac cattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cccggcgtca gtttcagccc
      421 cgaccgcaag tggatgatgc tcatggatca gccgggcaat ccttccattg aggagttgtc
      481 gcaacccgaa cttcgcttgg ccggtatccg catcaacccg cgcaccaacg gccccagcag
      541 aagcaattac tacaccaacc tgaccatcaa aaacatcgcc aacggcgccg aaaaaaaagt
      601 gcagcgcctg cccgccaagg ctcgcattca gtatgtgtcc tggtcgcccg acggctccaa
      661 agcggctttt gtgaacgcag tggaggcagg cttggagttg tgggtggtgg atgttgaagc
      721 tgccacggct cgtatctttt cctcccccgc agtgaatgct gccatgcccg gttctccgta
      781 tagttggatc ggcaatgacc agattctcat cagtatgatt ccgacaggac gaggccccgc
      841 gccgcaagcg ccccttacgc ctgccggccc catagtgcag gccaatgaag gcaaagccgc
      901 tcctgtgcgc acttttcaag atttgctgac caacgcccat gacgaagcgc tgtttgaaca
      961 ctacggcgcc tctcagctca tgttggtgga cctgaaacaa ggcacgagaa ccggcatggg
     1021 cgaagcgggt attatcaccg atttcaacgt ctcgcccgat ggcaaatatt tgctggtcac
     1081 tgctttgcaa aaaccgtttt cctacatcgt gccgtattcc cgctttccgc aaactacggc
     1141 catctggaac atgcagggcc aggtgctgaa agtgattgtt caaaaaccat taatggacaa
     1201 actgccgcag gggtttgacg ccgtagctac cgggccgcgc agcatttcct ggcgtgccga
     1261 tgcgccggct acgctgtact gggtagaggc gcaggatggc ggcgacccca aaaaagaagt
     1321 ggccgtgcgc gataaaatgt acgctctgga agctccgttc ggcgagcagc cgaaagaagt
     1381 catctccatc gccttgcgct atcggggcat cagttggggc aagggcgatc tggcgctggc
     1441 gtatgaaggc tggtggcaaa accgccgtca agtcacgagc atctggcagc ccgacaacac
     1501 agccgcaggc aaaacctcgc tttttgaacg ctcaacggaa gaccgctaca acgatccggg
     1561 cggatttgtg acacagctca acgaatatgg ccgctcggtg ctgctc
//
LOCUS       DAORLF010000664         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_39272_length_1595_cov_2.194805, whole genome shotgun sequence.
ACCESSION   DAORLF010000664 DAORLF010000000
VERSION     DAORLF010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39272_length_1595_cov_2.194805"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK198_27510"
     CDS             complement(<1..258)
                     /locus_tag="PK198_27510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42581.1"
                     /translation="MNFIEVKSKSDWKLFHRVPHRIYKNDPNWICPLQGDVQSIFEPA
                     TNKVFEHGEAKLFVLLDERNEPAGRVAAFIDHARNKNLPYPV"
     gene            complement(314..1501)
                     /locus_tag="PK198_27515"
     CDS             complement(314..1501)
                     /locus_tag="PK198_27515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42582.1"
                     /translation="MQIIEIQGAGMWKVFHSVPPRIYACDPNWVCPLERDIQSIFTPA
                     SNKAYEHGEAQLFVLLDDHAAPAGRIAAFIDHHHNQTQPYPVGGIGFFECIDNADYAA
                     ALFEKAESWLQAHGAQAVDGPVNFGERDRYWGLLDKGFDMPLFQENYQPRYYKAFFEN
                     NGYIPFEQILTLTGLTADIPFERMSAVANRIRRDHDVRIERYHPAHITRYAHDFCEVY
                     NASFRHFQHFKPIYPEKVRAFLHDARFILDPELLCIGYFEGKPAGFCLLLPDINPLLQ
                     PFRGKLNALNAMRFLWRRRTTQHFRAKGIGFGIHPDYKSKGLMAFFLEFMSSKHNLSY
                     YKKMYLTGVRAHNWEIRSIYDKMAVHTDRIHVAYRKALSPDIELEPFEFLETGKLLLQ
                     NED"
BASE COUNT          404 a          353 c          439 g          399 t
ORIGIN      
        1 cacaggatag ggaaggtttt tgttgcgggc atggtcaata aaagccgcca cgcgcccggc
       61 aggttcgttg cgctcgtcga ggaggacgaa aagtttggct tctccgtgtt caaaaacttt
      121 attcgttgcc ggctcgaaaa tggactgcac atcgccctgc aaaggacaaa tccagttggg
      181 gtcgtttttg taaatccggt gcggcacgcg atggaagagt ttccagtctg atttgctctt
      241 aacctcaata aaattcatag tcaggttgtc aatttgggcg gcaatttagg aaggtttgag
      301 caatgaaggc cattcaatct tcattttgca gcaggagctt gccggtttcg agaaattcaa
      361 agggttccaa ctctatatca ggagacagcg ccttgcgata agccacatga atgcggtcgg
      421 tatgaacggc cattttatca tagatgctgc gtatctccca gttgtgcgcc cgtacaccgg
      481 tgaggtacat ttttttgtaa taagaaaggt tatgttttga agacataaac tccaggaaga
      541 acgccatcag ccctttcgat ttgtaatcgg gatgtattcc gaaaccgatg cctttggctc
      601 tgaaatgttg agtagttcga cggcgccaaa ggaagcgcat cgcattcagc gcattgagct
      661 ttccccggaa gggctggagc agcgggttga tatccggcaa aagcaagcag aacccggcgg
      721 gttttccctc aaagtacccg atgcagagca actccggatc gagtatgaaa cgggcgtcgt
      781 ggaggaaagc tctgactttt tcgggatata tgggtttgaa gtgctggaaa tgccggaagc
      841 tggcattata aacctcgcag aagtcatggg catagcgggt gatatgtgcc gggtggtagc
      901 gctctatacg cacatcgtga tcgcggcgaa tgcggttggc aacagcgctc attctttcaa
      961 aaggaatatc tgcggtcagt ccggtgaggg tcagtatttg ttcaaatgga atgtacccgt
     1021 tgttttcaaa aaaagcctta tagtagcgcg gctggtagtt ctcctgaaac aaaggcatgt
     1081 caaaaccttt gtccaacaag ccccaatacc tgtcgcgttc accaaaattg acggggccgt
     1141 ccactgcctg agcgccatgt gcttgaagcc aggattctgc tttttcgaag agggcagcgg
     1201 cgtagtcggc gttgtcaatg cactcaaaaa agccgatccc acctaccgga taaggctggg
     1261 tttggttgtg atggtggtca ataaatgctg caatgcgccc ggccggcgcc gcgtggtcgt
     1321 cgagtaaaac gaagagttga gcttcaccgt gttcgtaggc tttgttgctt gcgggagtaa
     1381 aaatggactg gatatcacgc tctaaaggac aaacccaatt gggatcgcag gcatagattc
     1441 ggggtggcac gctgtggaat accttccaca ttccggcgcc ttgaatttct atgatttgca
     1501 tggcgtttat tcgctgtatt gagcgaacaa attataggaa gtcagaggaa agaaaaatga
     1561 caaaccgtcc ggacagcaga tgatttttat cacca
//
LOCUS       DAORLF010000665         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_39968_length_1571_cov_1.560026, whole genome shotgun sequence.
ACCESSION   DAORLF010000665 DAORLF010000000
VERSION     DAORLF010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39968_length_1571_cov_1.560026"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1208)
                     /locus_tag="PK198_27520"
     CDS             complement(84..1208)
                     /locus_tag="PK198_27520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRF42583.1"
                     /translation="MKFPWLITAVMVLHSVLSFGAPPREMSESEWTASNERDIQLRLE
                     QLRLPFSIQTNNSRIIRYIHDYAAVGIQETETMLGRSNLFLPVFEHYLSIYQLPQELK
                     YLPIVESGLKTDTQSPAGARGLWQLMEVTARQYKLRVSGGTDERLDPYRSTEAAMRLL
                     SDLYDQYQDWPLTIAAYNCGPGRVNKAIRLARSKDYWKVSKHLPAETQRYVPAFVAAA
                     YIHKHHADHGLAPRGGEEAEQGVRVFHISTTMSISQIANACETETNVIRNLNPAFPGN
                     VISASDKGFYLAVPMASSKPFQEWLSRQKAYNHALFADKKALHTTYVTAPGDVIDAVA
                     RRFQCTVEDIMRWNNLKTAEIVVHQELNIYLTREFLLGRV"
BASE COUNT          364 a          357 c          429 g          421 t
ORIGIN      
        1 caatttcccc aaaacagcaa aggccacaga gaagttgatg cttcgctgcg gcctttgcca
       61 aaccggtgtg ctttctcaga gtattacacc ctgcccaaaa ggaactctct ggtgagatag
      121 atattcaact cctgatgaac tactatctcg gctgttttca gattgttcca gcgcatgatg
      181 tcttccacag tgcattgaaa gcggcgcgca acggcgtcaa tcacatcacc gggagctgta
      241 acgtaagtag tatgtagagc cttcttgtca gcaaataatg cgtggttgta agccttttgg
      301 cggctcagcc attcctgaaa aggcttgctg gaagccatgg gaacagcgag gtagaaccct
      361 ttgtcggaag cagaaatgac attgcccgga aacgcaggat tcagattgcg gatcacattg
      421 gtctccgttt cacaagcatt ggcaatctgg ctgatagaca tggtggtaga gatgtggaac
      481 acccgtacgc cctgctctgc ttcttcgccg ccgcgaggcg caagtccgtg atcggcatgg
      541 tgtttgtgga tgtatgccgc cgccacaaaa gcaggcacat agcgctgcgt ttctgccggg
      601 aggtgcttgc ttactttcca gtagtctttg ctgcgagcca agcggatggc cttgtttacc
      661 cggcctgggc cacaattgta tgcggcgatg gtgagcggcc aatcctgata ttggtcgtag
      721 aggtcagaca acaaacgcat ggctgcctcg gtagagcgat aaggatcaag gcgctcgtct
      781 gtaccgccgc tgacgcggag tttgtactga cgggccgtca cttccatgag ttgccacagc
      841 ccgcgggcgc ctgccggcga ctgggtatcg gttttgaggc cggactcaac gatggggagg
      901 tatttcagtt cttgaggcag ttgataaatg ctgaggtagt gttcaaaaac gggcaaaaac
      961 agattgctgc ggcccagcat cgtttcggtt tcctgtatgc ccacggctgc gtagtcgtgg
     1021 atgtaccgaa tgatacggct gttgttggtt tggatggaga acggaagtcg gagttgttcg
     1081 aggcgcaatt gaatatccct ttcgttggat gctgtccatt cggattcaga catttcacgc
     1141 gggggggcgc cgaaacttag cacgctatgg agtaccataa cggctgtaat caaccatggg
     1201 aatttcatcg tttatcttgt tttgttcatg ggtgttttcc ttttcaggct tcgtcgatgt
     1261 cgttgtaggt ccagcaaagg tatgggcatt tacgactccg cgcaatattt tgttgcataa
     1321 atgccgggca agacagaaaa ttcaatttca gaaagcagtg gaaaagccgg ggttcaccaa
     1381 atataattag ccgagcgctc gtactttgct ccagagtggt gttttcttaa caattgcttc
     1441 ccgttttttc cttcggaatg ttaaaatttg aaaaaaaatg ctttttgctt cagttttttg
     1501 atggaaagcg aaagaaaaaa tgggtttcgc ctatgattga acatggctgt ttgttgatat
     1561 caatccatct g
//
LOCUS       DAORLF010000666         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_40022_length_1569_cov_2.764201, whole genome shotgun sequence.
ACCESSION   DAORLF010000666 DAORLF010000000
VERSION     DAORLF010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40022_length_1569_cov_2.764201"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PK198_27525"
     CDS             complement(<1..510)
                     /locus_tag="PK198_27525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42584.1"
                     /translation="MRIWILFLLLLPGAAGYGQGSPCEIGVYQELITAGNSYFKMSRL
                     DKALECYALALDVCPEKEAEVDSLIQTVFQRIRHIELTTDVTYVPILKFGGKGHERLF
                     YYVDGRGVVLKHLGHWKIAGLFSTYGALATVKVKNGSKYDTFLLDTEGNKYPAAYELN
                     ELNEGITALD"
     gene            complement(613..>1569)
                     /locus_tag="PK198_27530"
     CDS             complement(613..>1569)
                     /locus_tag="PK198_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF42585.1"
                     /translation="QLTTLPAGIGKLTRLNYVHLSHNQLKNLPNEIGQWSEVISLNLS
                     NNQLTALPLGIEKMTKLTRLTLTKNQLKTLPHEIGQLTELTHLHLENNQLTALPLEIW
                     RLTKLRKLDLTNNQLSERPTEAENLPYYTSVDWSGNPIKETTAASDSPPKAIMSPREQ
                     EEMQGSMAAPKVPNESMATPKVAGSMAPPPPPPAPTTREIFTAVEQMPRFPGCENEAD
                     LFAKNDCSNNKVKDFISKNMKYPAIARENGVQGTVVITFVVEKDGSLSDIKLVRDIGA
                     GCGQEALRLVNLMQEQGLRWTPGQHQGNTVRVQYNLPVKFKL"
BASE COUNT          361 a          401 c          372 g          435 t
ORIGIN      
        1 gtctaatgca gtaatgccct cgtttaattc attcaattcg taagccgccg ggtatttatt
       61 gccctcggtg tctaataaga acgtgtcgta tttgctgccg tttttaactt tcacggtggc
      121 cagtgctccg taggtgctaa aaagccctgc gattttccag tgtccgaggt gcttcagaac
      181 aacgcccctt ccgtcaacat aataaaaaag ccgctcgtgg cctttgccgc caaatttcaa
      241 aatgggaaca taggtcacat ccgtagtaag ttcgatgtgc cgaatgcgct gaaataccgt
      301 ctgtatcagg gaatctacct ctgcctcctt ttcagggcag acgtccagcg ccagagcgta
      361 acactcgagt gccttatcca acctactcat tttaaaatag ctattaccgg cggtaatcag
      421 ttcttgataa accccaattt cgcagggact accttgcccg taccccgccg cgcccggcag
      481 caacagcaaa aacaagatcc aaatgcgcat catcattttt gattttgaat gagaacaaaa
      541 gtaacacaga aaagtcggca ggccaagctg ctgaggccac tccgcgacgc cacccgccca
      601 tcacatcccc gctcacaact tgaacttcac cggtagattg tactgcacac gcaccgtatt
      661 gccctgatgt tggccgggtg tccagcgcag gccctgctct tgcatgaggt tgacaaggcg
      721 caaggcttcc tgtccgcatc ctgcgccgat gtctcggact aatttgatgt cagacagcga
      781 gccgtctttt tccacaacga aagtgatgac gaccgtgcct tgtacgccgt tttcgcgggc
      841 gatggcagga tatttcatgt ttttgctgat gaagtcctta actttattat ttgaacagtc
      901 attcttggca aagaggtcgg cttcattttc gcagccggga aagcgaggca tttgttcaac
      961 tgctgtgaaa atttcacgag tggtaggagc cggcggcggc ggcggcgggg ccatactacc
     1021 tgcaacctta ggggttgcca ttgactcatt cggtaccttc ggcgccgcca tcgaaccttg
     1081 catttcctct tgctctcgtg gagacataat agcttttgga ggactatccg atgccgccgt
     1141 agtttctttt atcggattgc cggaccaatc aacagatgtg taataaggca aattttcggc
     1201 ttcagttggt cgttccgaca gttgattatt ggtcaaatcc aatttcctca gcttggtcaa
     1261 tcgccatatt tctaaaggga gggcagttaa ttggttattt tccagatgga gatgagtcaa
     1321 ttccgtcagt tgcccgattt cgtgcggcag cgttttcaac tggtttttgg tcagtgtcaa
     1381 tcgagtcaat tttgtcattt tttcgattcc taaaggcagt gcggtcagtt ggttgttgct
     1441 cagattcagc gagataacct ccgaccactg cccgatttca ttcggcagat tttttaattg
     1501 attgtgggac agatgcacat aattcagcct cgtcagttta ccaataccgg caggcaatgt
     1561 cgtcagttg
//
LOCUS       DAORLF010000667         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_40029_length_1569_cov_1.640026, whole genome shotgun sequence.
ACCESSION   DAORLF010000667 DAORLF010000000
VERSION     DAORLF010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40029_length_1569_cov_1.640026"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>315
                     /locus_tag="PK198_27535"
     CDS             <1..>315
                     /locus_tag="PK198_27535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42586.1"
                     /translation="YIRDAQGCVVVRTFTISYSSGNFSFTGTPTAATCNAPGSILVNT
                     SNGTPPYNITVSGPVSGNLTSNTNQFTIANLPGGTYTVTIGSSGQCQFTRTIVVPNTG
                     LQV"
     assembly_gap    316..332
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <333..>1569
                     /locus_tag="PK198_27540"
     CDS             <333..>1569
                     /locus_tag="PK198_27540"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF42587.1"
                     /translation="QSGACNQPGSIAVQVQSGTAPYTITWNGPSSGSITTGNANYQIA
                     GLPSGSYGVQVRDATNCLRYEVVNIQNSSATLSATFSAISSGCTATGSIAIQINNGNP
                     QYVISWSGPVSGTITTSSPNYTISNLPGGAYLMQVTDASGCSRTGMVSVSAGNGLSVT
                     PTVNNAGCGQNGSINLAIAGGTAPYSISWSGQVSGSATTSSATYTIPNLPGGTYSIIV
                     TATGGCQWQQNVTVTSSGGGNFSVSLMPIAPSCQTCAAIWVDIYGGQAPYTLTWSGPT
                     PGSLVTSQTNFDIYCVNPGTYTVVVTDANGCSTSGTVVISPSSTSLQINTNITPADCI
                     MSASGSVYFNILNGQPNFTVIWTGPVSGNWNTSLREFSIYNLPSGNYTFTITRTSTLT
                     IDKTSTYTAPLKAGDTVTYG"
BASE COUNT          356 a          456 c          383 g          357 t
ORIGIN      
        1 ctacatccgc gatgcgcaag gctgtgtggt ggtcaggaca tttaccattt cttattcgag
       61 cggcaacttt agttttacgg gtacgcctac tgccgccacc tgtaacgcgc ccggctctat
      121 tttggtaaat acaagcaatg gtacgccacc atataatatt acggtaagcg gcccggtgag
      181 cggcaatttg accagcaaca ccaatcagtt taccattgcc aacctgccgg gcggcaccta
      241 caccgtcacc atcggttctt cggggcaatg ccagttcacc cgcaccatag tagtgccgaa
      301 cacgggcttg caggtnnnnn nnnnnnnnnn nnggcaaagc ggcgcctgca atcagccggg
      361 cagcatcgcc gtgcaggtac aatcaggtac ggcgccctat accatcacct ggaacggccc
      421 ttcgtcgggg agcatcacca ccggcaatgc gaattatcag attgcggggc ttccttcggg
      481 cagttacggc gtgcaggtga gagatgccac aaattgtttg cgctatgaag tagtgaacat
      541 acaaaactca agtgcgactt tgtctgccac tttttccgcc atcagttcgg gctgtaccgc
      601 cacgggttct attgccatac aaatcaacaa cggcaacccg cagtatgtca tttcctggag
      661 cggtccggta tcgggtacca taaccacatc ttcgcccaac tacaccatat ccaacctgcc
      721 gggcggcgca tacctcatgc aggtaacgga tgcctccggc tgtagccgaa caggcatggt
      781 gagcgtcagt gcaggtaatg gattatccgt gacaccaaca gtgaataacg ccggctgtgg
      841 ccagaacggc tctatcaatc ttgccatagc aggaggcaca gcgccttatt ccatttcctg
      901 gagcggtcag gtttccggca gtgcgacgac atccagcgct acttatacga ttcccaactt
      961 gcccggcggc acctacagca tcatagttac cgcgaccgga ggctgtcagt ggcaacaaaa
     1021 tgtgaccgta accagtagcg gcggcgggaa tttctctgtt tctttaatgc cgattgcgcc
     1081 atcttgccaa acttgtgccg ccatctgggt ggatatttac ggagggcaag caccctacac
     1141 gcttacctgg tcgggtccga cgcccggcag cttggtgacg agccagacaa attttgacat
     1201 ctattgcgtc aatccgggta cttatactgt ggtcgttacg gatgccaatg gttgcagtac
     1261 ttccggaaca gtggtcattt ctccaagtag taccagcctt caaatcaata ccaacataac
     1321 tcctgccgat tgtatcatgt ctgcatcggg gagtgtgtat ttcaatattc tcaacggcca
     1381 gcccaacttt accgtgattt ggaccggccc cgtttccggc aactggaaca cttcattgcg
     1441 ggagttttct atttataacc tgccttccgg caactacacc ttcaccatca cccgcacctc
     1501 gaccctgacc attgacaaga catcgaccta tacggcgccg ctcaaggccg gcgataccgt
     1561 tacctacgg
//
LOCUS       DAORLF010000668         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_40336_length_1558_cov_2.312708, whole genome shotgun sequence.
ACCESSION   DAORLF010000668 DAORLF010000000
VERSION     DAORLF010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40336_length_1558_cov_2.312708"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PK198_27545"
     CDS             complement(<1..701)
                     /locus_tag="PK198_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF42588.1"
                     /translation="MQTGRISVQTENIFPIIKKYLYSDQEIFLRELVSNAVDATVKLK
                     ALAGKGAVQGDIGDTTIDILLDADNKTLTIRDRGLGMTAEEVEKYLNQVAFSSAHDFI
                     EKYKLDHNIIGQFGLGFYSAFMVADRVEAVTKSYIADEPGVTWSCDGSPEYTLTPNDK
                     QERGTDIVLHISEDAVEYLESSRIEGLLKKYCRFLPVSIRFGTRTETTYEGEGEDEKA
                     IDTEVENIVNNPSPA"
     gene            complement(1182..>1558)
                     /locus_tag="PK198_27550"
     CDS             complement(1182..>1558)
                     /locus_tag="PK198_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF42589.1"
                     /translation="EDLRRTRIIVHLGVTVVMFVMILIFETMNDQAVITKLFQIAGYT
                     YGPLLGLFVFGMTTRLKVRDHLVMVVCILAPIFTFGLDTYSTQILAGFKFGFLTIALN
                     GLLTYLGLLAISYWDTEVAVKE"
BASE COUNT          390 a          371 c          405 g          392 t
ORIGIN      
        1 caagccgggc tggggttgtt cacaatgttt tctacctcag tatctattgc cttttcgtct
       61 tcgccctcgc cttcataagt ggtttcggtg cgggtgccga agcggatgga aacgggcagg
      121 aagcggcagt attttttgag cagcccctct atgcgggacg attcgaggta ctctacggcg
      181 tcttcgctga tgtgcaacac gatgtcggtg ccgcgctcct gcttgtcgtt gggcgtgagg
      241 gtgtattccg ggctgccgtc gcaagaccag gtaacgcccg gctcatcggc gatgtacgat
      301 ttggtgacgg cctctacgcg gtcggccacc ataaatgcag agtagaagcc gaggccgaac
      361 tgcccgatga tgttgtgatc gagcttgtat ttttcgataa aatcgtgggc gcttgagaaa
      421 gccacctgat tgaggtactt ctccacttct tcggcggtca tgcccaatcc ccggtcgcgg
      481 atggtgaggg ttttgttgtc ggcatccagc aaaatgtcaa tggtggtatc gccgatgtcg
      541 ccctgcacgg cgcctttgcc ggccagcgct ttgagtttta cggtagcatc aacagcattg
      601 gaaaccaatt cgcgcaggaa gatttcctga tcggaataca agtacttctt gatgatggga
      661 aaaatgttct cggtctgaac agatatgcgt cctgtttgca tggcggtcat tttttttcag
      721 aggcattgtc aaaccttgtg ccattgttga ctgactgaca aattgtctga aaaaaaatat
      781 ttgcaccata aatatatagg acttatcttt gcagcatcta cacactaatt gaggcgttgc
      841 ggtacgcaag tactacccca aagagatgac cgaaaatcat tcggagcttg ggtttaaagc
      901 atttccgctt tagttatcta cataccaaaa cataagttga ttcattgttt ttctgcgctt
      961 ggcacaggga ggcaatgctt ttaggggtag tttgtgcaag tggaagcaag ccccccttat
     1021 cgcaatacac agatcagaga tcactacgcg atcataaaac aactttgcac aagcaatcaa
     1081 acaaacctgg cagaagcgct tgcgcttttc cagggagtga gggaggccgg tttatcgggg
     1141 ggtaaggccg gtcttccact cactcaaatg aataaaaaac atcactcctt caccgccacc
     1201 tccgtatccc aataagaaat agccagcagg ccgaggtaag tcagcaagcc gttgagtgca
     1261 atggtgagga agccgaattt gaaaccggcg agaatctgcg tcgaataagt gtctaaacca
     1321 aaggtaaata tgggcgccag gatgcacacc accataacca gatgatcgcg cactttgagg
     1381 cgggtcgtca tgccaaatac aaacaatccc aagagcgggc cgtaagtgta tccggctatt
     1441 tggaacagct tggtaataac cgcctgatcg ttcatggtct caaaaatgag gatcattaca
     1501 aacattacaa ccgtgacacc gaggtgtacg atgatgcgcg ttctgcggag gtcttctt
//
LOCUS       DAORLF010000669         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_40978_length_1538_cov_1.608901, whole genome shotgun sequence.
ACCESSION   DAORLF010000669 DAORLF010000000
VERSION     DAORLF010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40978_length_1538_cov_1.608901"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>803)
                     /locus_tag="PK198_27555"
     CDS             complement(<1..>803)
                     /locus_tag="PK198_27555"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF42590.1"
                     /translation="RAGALRLMLYMGLADKVGFIEKNDLARVVPYMMAHPELKQRPVI
                     GVSNNFDPEAVAVSGTSLIVGTYMSEEEADDWTIKTGKPVFCLAYGDLYHLKTDFYKS
                     LTALGQLFDRAQRADSLIAYIETTISEVGKRAKRSQRRGHSAYIGGIAFNGVQGIAST
                     RAQYPPFVYLGIDSPADALPHSIESIGMGQKNMLIDMEQLISWDTDYLFLDASGQPAW
                     SEEINKPVLARLRAKKLQQTYVLLPFNWHTINYEHLLCNMWFVGKTVYP"
     assembly_gap    804..948
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1039..1122)
                     /locus_tag="PK198_27560"
     tRNA            complement(1039..1122)
                     /locus_tag="PK198_27560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1086..1088),aa:Leu,seq:gag)
     assembly_gap    1166..1307
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1308..>1538)
                     /locus_tag="PK198_27565"
     CDS             complement(<1308..>1538)
                     /locus_tag="PK198_27565"
                     /inference="COORDINATES: protein motif:HMM:NF022060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HRF42591.1"
                     /translation="PREQSELYFQSRPLGSRIGAAASPQSEVVDRAVLEADFMRLQAE
                     AEAAGGTFPCPPHWGGYKIIPSRFEFWQGRISR"
BASE COUNT          288 a          331 c          333 g          299 t
ORIGIN      
        1 cgggatagac ggtttttcct acaaaccaca tattgcacag cagatgctca tagttgatgg
       61 tgtgccagtt gaagggtaat aaaacgtagg tttgttgcag cttttttgca cgaagccggg
      121 ccagaaccgg cttgttgatt tcctccgacc aggcaggctg tccggaggcg tccaaaaata
      181 aatagtctgt gtcccaactg atgagctgct ccatatctat cagcatattt ttctgtccca
      241 tgccgatgct ctctatactg tgaggaaggg catccgccgg actgtcaatg ccgagataca
      301 caaaaggcgg atactgagcg cgggtagaag caatgccctg aacgccgttg aaagcgatgc
      361 cgccgatgta ggccgaatgc ccgcgccgct gtgaacgttt cgcccgcttg cccacttctg
      421 agatggtggt ttcgatgtaa gcaatgaggc tgtcggcccg ttgtgcgcgg tcgaaaagct
      481 gccccagcgc agtgaggctt ttgtaaaagt ctgttttgag gtggtacaag tcgccataag
      541 cgaggcaaaa gacgggcttt ccggttttta tcgtccaatc gtcggcttct tcttcgctca
      601 tataagtacc gacaatcaag gaggtacccg aaaccgccac ggcctccggg tcgaaattgt
      661 tgctcacacc aatgacgggc cgttgtttca gttcgggatg cgccatcatg taaggtacta
      721 ctctggcaag gtcgtttttt tcaataaatc ctactttgtc ggccagcccc atgtacagca
      781 tcaatcggag tgcgcccgcc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ttcgtatttg
      961 gcgaggcaat gatatacatt tggcggggtt taaagataga gccaataaaa aaagccgctc
     1021 cattcggaac ggcttttgtg cccagaacag gattcgaacc tgcacaccct tgcgagcact
     1081 accccctcaa agtagcgtgt ctaccaattc caccatctgg gcggggagaa aaatgctttt
     1141 tcggagctgt aaatggagag ggttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ctgattcgcc
     1321 cttgccaaaa ctcaaatcgg gaaggaataa ttttgtatcc accccagtga ggcgggcagg
     1381 ggaaagtgcc gcctgctgcc tccgcttctg cctgtaatcg cataaaatcc gcctccagca
     1441 cggctctgtc caccacctcg ctttgcggag atgcggcggc gcctatgcga ctgcccaaag
     1501 gccggctttg gaaatacaat tccgattgtt cgcggggc
//
LOCUS       DAORLF010000670         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_41040_length_1536_cov_2.243754, whole genome shotgun sequence.
ACCESSION   DAORLF010000670 DAORLF010000000
VERSION     DAORLF010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41040_length_1536_cov_2.243754"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PK198_27570"
     CDS             <1..>1250
                     /locus_tag="PK198_27570"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRF42592.1"
                     /translation="PLLEKSIVPNLLYDAELTENFRQEMLAGVSEYRGVVKKGELIAA
                     RNSIVTEDIWLEINSVQELYDKEIAGRFSPSGIGAGYFILALLLCTVYFIYLQNREPS
                     ILSNWRKLVFTLFWIALYAWLIHWAEETNPINAYFIPFSIAPIILQTFYNKRLALFTH
                     VIIILTASFITSLGWDFTFLQLLAGIVVLLTNVDVNSWPKFFFSIFSMLVASLLAFTG
                     LTLVEHGNLFSWNWTILTWIFLSAFLTLLALPLVPLLESIFGFVSAFRLRELMDLNRP
                     LLRELAIKAPGTLQHSIQVGNLSEAAAREIGADALLLKCAALYHDVGKMLHPEYFVEN
                     QSGIESPHEEKSNLDSARIIISHVTEGEKMARKSGMPPLLIDFITTHHGTTRVEYFYR
                     KMLNESSDEMPDELDFLYPGPKPH"
     assembly_gap    1251..1260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1261..1506
                     /locus_tag="PK198_27575"
     CDS             <1261..1506
                     /locus_tag="PK198_27575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRF42593.1"
                     /translation="KEQTIFMMADSIEAACRSLKQPTEQSMNELIDNIIAGKIKMGQL
                     EESPMSFNELERCRAAFKRFMRSVHHQRIEYPELKKE"
BASE COUNT          358 a          416 c          375 g          377 t
ORIGIN      
        1 atccgctttt ggaaaaatcc attgtgccca atctcctcta cgatgccgaa ctgaccgaaa
       61 atttcagaca ggaaatgctg gccggcgttt ccgagtacag aggcgtcgta aagaagggcg
      121 agttgatcgc cgccagaaac agcattgtga cggaagatat ttggctcgag atcaactctg
      181 tacaagaact ttacgacaaa gaaatagccg ggcgcttctc tccatcgggc ataggcgcgg
      241 gttatttcat cctggccttg ctgctctgta ccgtgtattt tatttacctc caaaaccggg
      301 agccgtccat attgtccaac tggcgcaagc tcgtttttac gctgttttgg atagcgctgt
      361 acgcatggct catccactgg gcggaagaga ccaatcccat caatgcttat ttcattccgt
      421 tcagcattgc acccattatt ttgcagacct tttacaacaa aaggctggca ctttttacac
      481 acgtcatcat cattctgacg gcgagtttca tcacgtcgct gggctgggat ttcacctttt
      541 tacaactgct ggccggcatc gtcgtgctgc tcaccaatgt ggatgtgaat agctggccca
      601 agtttttctt ctctattttt tccatgctcg tggcctcttt gctggcgttt accggcctga
      661 cattggtgga acacggcaac ctgttttcct ggaattggac catcctcacc tggatatttt
      721 tatcggcatt tcttacgctg ctggccctgc ccctggtgcc cttgttagag agcattttcg
      781 gcttcgtttc ggcgttcaga ctgcgggaac tcatggacct caatcgtccg ctgctgcggg
      841 aattggccat caaagcgccg ggcaccctgc aacactccat tcaggtgggc aatctgagcg
      901 aggccgccgc ccgcgagatc ggcgccgatg cgctgctgct caaatgcgca gccttgtacc
      961 acgatgtggg caagatgctg caccccgaat attttgttga aaaccagtcg ggtatagaaa
     1021 gtccgcacga agaaaaatcc aatttggaca gcgcccgcat catcatcagc catgtgacgg
     1081 aaggcgaaaa aatggcccgc aaaagtggca tgcctccctt gctcattgat ttcattacga
     1141 cgcaccacgg cacgacccgg gtagaatatt tttacagaaa aatgctcaac gaaagttcgg
     1201 acgaaatgcc cgacgagctt gattttcttt accccggccc caagccgcat nnnnnnnnnn
     1261 aaagaacaaa ccatctttat gatggccgac tccattgaag ccgcctgccg cagcctcaaa
     1321 caacccaccg agcagtctat gaacgagctg attgacaaca tcatcgcggg caagatcaaa
     1381 atggggcagt tggaagaatc gcccatgtcg ttcaacgagt tggaacgctg ccgcgccgca
     1441 ttcaaacgct tcatgcgttc cgtacaccat cagcgcatcg agtacccgga attgaagaag
     1501 gagtagggtg ggggatttgt gtttgggcgg ctcact
//
LOCUS       DAORLF010000671         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_41077_length_1535_cov_1.880405, whole genome shotgun sequence.
ACCESSION   DAORLF010000671 DAORLF010000000
VERSION     DAORLF010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41077_length_1535_cov_1.880405"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>366)
                     /locus_tag="PK198_27580"
     CDS             complement(<1..>366)
                     /locus_tag="PK198_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HRF42594.1"
                     /translation="GQAPDYAAELTSWLHSFTPAINYYLREELNFKTDIKYNMFGPVN
                     PWDNSNDNTRDNLRQAMAQNPYLHVMFQSGYYDGATTYFNAKYTMWQIDPAGRMKDRF
                     SFKGYRSGHMMYLRRDDLR"
     assembly_gap    367..455
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<456..>1043)
                     /locus_tag="PK198_27585"
     CDS             complement(<456..>1043)
                     /locus_tag="PK198_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HRF42595.1"
                     /translation="LDVADIVFINPANTGYSRTIPAAGKEVDRSKFFGINADIAYLAE
                     WLNTFVSRHNRWRSPKYLIGESYGGTRVSGLALELQNRQWMYLNGVILVSPADYKVIR
                     EDGPVSQALHLPYFTATAWHHKLLSPELQQKDLLDILPQAEDFTINTLIPALAKGGFI
                     SESEKMEVAKKIAYYSGLSEKEVLEHNLAVPTNYFW"
     assembly_gap    1044..1131
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1132..1439)
                     /locus_tag="PK198_27590"
     CDS             complement(<1132..1439)
                     /locus_tag="PK198_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42596.1"
                     /translation="MYRTLTFALIFMLVAGWSAYAQPRLVLPLDTMVVTKHTVTIGGA
                     LVPYTAVTGTQPVWDEAGQPIASLNYTYYSRSDVKKQDERPLVLSFNGGPGSASVWMQ
                     "
BASE COUNT          296 a          333 c          407 g          322 t
ORIGIN      
        1 tgtctcaggt catcgcggcg caggtacatc atgtggccgc tgcggtaacc tttgaagcta
       61 aaccggtctt tcatacggcc cgcaggatca atttgccaca tggtgtattt ggcattgaaa
      121 taggtagtgg cgccatcgta gtagcccgac tgaaacatta catgcaggta aggattttgg
      181 gccatagcct gccgcaggtt gtcgcgggta ttgtcgttgc tgttgtccca gggattgacc
      241 gggccgaaca tattatattt gatgtcggtc ttgaaattca actcctcccg caggtagtaa
      301 ttgatggccg gggtgaagga atgcagccag gaggtcagtt cggcggcgta gtcgggcgcc
      361 tgtccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaga agtagttggt cggcacggcc
      481 aggttgtgct ccagcacttc tttttcggaa aggccggaat agtaggcgat ctttttggcc
      541 acctccattt tttcagattc ggaaataaaa ccgcctttgg ccaaagccgg aatgagcgta
      601 ttgatggtga aatcttcggc ctgcgggagg atgtccaaca agtctttttg ctgaagctca
      661 ggacttaaca acttgtggtg ccaggccgta gcagtgaaat aaggcaggtg caaggcctgc
      721 gataccggcc cgtcctcgcg gatgactttg tagtcggccg gcgaaaccaa gatgaccccg
      781 ttgagataca tccattgccg gttttgcagt tccaaagcca gccccgatac ccgcgtgccg
      841 ccgtagcttt cgccgatgag gtacttgggc gaacgccagc gattgtgacg ggagacgaag
      901 gtattgagcc attcggccag ataggcaata tcggcgttga tgccgaaaaa tttgctgcgg
      961 tccacctctt tgcccgcagc aggaatggtg cgcgaatagc ccgtgttggc ggggttgata
     1021 aacacaatat cggccacatc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naattgcatc
     1141 cacaccgacg cagagccggg gcctccgttg aacgaaagga ccaaagggcg ctcgtcctgt
     1201 ttcttcacgt cgctgcgcga gtagtaggtg taattcagcg atgcgatggg ctgccctgcc
     1261 tcgtcccaca cgggctgcgt gccggtgaca gcggtgtagg gtaccagcgc gccgccaatg
     1321 gtaaccgtgt gtttggtgac aaccatcgtg tccaaaggca agacgaggcg cggctgcgca
     1381 taagccgacc agccggcgac gagcatgaaa atcagtgcga aggtgagtgt tctgtacatg
     1441 atgtttttga gtttgaaaaa tggcaatttt gagtgcggtg cgaaaaatag gtcttttacg
     1501 actgcgacgc ctgttttttg cggctgattt ttatg
//
LOCUS       DAORLF010000672         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_41442_length_1523_cov_1.880790, whole genome shotgun sequence.
ACCESSION   DAORLF010000672 DAORLF010000000
VERSION     DAORLF010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41442_length_1523_cov_1.880790"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK198_27595"
     CDS             complement(<1..716)
                     /locus_tag="PK198_27595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF42597.1"
                     /translation="MTIKFKSNVTDKEIASFETTHRLKMLRRAATGFIDYEIAPVQDI
                     VVLHDELKGVEVIEAIQLNYLGNWYFQPNDPLVSLQWYLDKIQAFAAWDNSRGSQEIV
                     VAVLDTGIDWSHPDFAGAIYSNSAEIPGNGLDDDDNGFVDDIHGWNFENNNSNINDLS
                     GTQSFHGHGTRVSGIIGAQLNNSIGIAGIGGGIRIMPVKVGSEGPLSSIVDDAILYAA
                     QNGAKIINMSFGWQSVVEDNP"
     gene            complement(1066..>1523)
                     /locus_tag="PK198_27600"
     CDS             complement(1066..>1523)
                     /locus_tag="PK198_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRF42598.1"
                     /translation="KSHDGYIGNSVIGEWCNLGADTNCSNLKNTYEEVRLWNYPKERF
                     LPTGRQFCGLFMGDFSRTGINAMLNTGTVIGISANVYGDGFPRNFIPSFAWGGASGFQ
                     TFRIDKAFNSAENIMSRREIPFDVGDRLIMLRVYEDSAQYRVWEKEPRP"
BASE COUNT          422 a          354 c          351 g          396 t
ORIGIN      
        1 gggttatctt caacaacaga ttgccatcca aaagacatgt tgataatctt cgctccgttc
       61 tgtgctgcgt ataaaatggc atcatctact atactggaca aaggtccttc gcttccgact
      121 ttcaccggca ttatccgaat gcctcctcca atgcccgcaa taccgataga attgtttaac
      181 tgtgctccga taattcccga aacccttgtg ccatgaccgt gaaaagattg cgttcctgaa
      241 aggtcgttga tattactatt gttgttctca aaattccaac catgtatatc gtccacaaag
      301 ccattatcat catcatctaa tccgttgcct ggaatttcgg cactattgct gtaaatagct
      361 ccggcaaaat cagggtgact ccaatctata ccagtatcca atacagcgac aactatttcc
      421 tggcttcctc tggaattatc ccatgcagca aaagcctgaa tcttatccaa ataccactgt
      481 agggaaacta atgggtcatt gggctggaaa taccagttac ctaaataatt caactgaatt
      541 gcttctatga cttctactcc ttttaattca tcgtgtagca ccacaatatc ctgtacggga
      601 gcaatttcgt agtcaataaa tccggtagcg gcgcgtcgga gcattttgag gcggtgcgta
      661 gtctcgaaag aggcaatctc tttgtcagtt acgtttgact tgaatttaat agtcaaaact
      721 ttgttgtcca cttctgcccc ccccccgaac aattgtttgg gagcctactt gagtggtcgg
      781 ccatacggcc tgaagtagaa aaagaatgat aaaacggagt aatgttctca taaaaaattg
      841 attataaagg tgaagaaaat acggagtttc actcttggaa ccattcatta ttatacgact
      901 atgcaaatat gataaagaaa ttttataaaa agaacagaat ggtaaaattt aacataaaac
      961 acacactaca aagcgcagac aggtataaac aggctgtacc catcgttaca aagtgcgtaa
     1021 tcggtaagaa gtaaatcaga ggaagggggc ttggtcgagt ggctcctacg gcctcggctc
     1081 tttttcccaa acccggtatt gcgccgagtc ttcgtacacc cgcagcatga tgaggcggtc
     1141 gcccacgtcg aaggggattt cgcggcggct catgatgttt tcggccgagt tgaaggcttt
     1201 gtcaatgcgg aaagtctgga acccggaggc gccgccccag gcaaaggacg ggatgaaatt
     1261 gcggggaaaa ccgtcgccat acacattggc agaaatgccg atgacagtgc cggtattgag
     1321 catggcgttg atgcccgtgc gggagaagtc gcccatgaac aggccgcaaa actgccgtcc
     1381 ggtgggcaaa aagcgctctt tggggtagtt ccagaggcgc acctcttcgt aagtgttttt
     1441 gaggttagag cagttggtat cggcgcccag attgcaccat tcgccgatga cggaattgcc
     1501 gatgtagccg tcgtgcgatt tat
//
LOCUS       DAORLF010000673         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_41874_length_1509_cov_1.648556, whole genome shotgun sequence.
ACCESSION   DAORLF010000673 DAORLF010000000
VERSION     DAORLF010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41874_length_1509_cov_1.648556"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>594)
                     /locus_tag="PK198_27605"
     CDS             complement(9..>594)
                     /locus_tag="PK198_27605"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRF42599.1"
                     /translation="ETRYRNVPRWQIAIELKRQFPDLPIICDNSHICGRRDTLQDVAQ
                     QALDLNYDGLMTEVHPTPDQAWSDAAQQITPVQYQELTQRLNFRLSGAEDPAFLENID
                     HLRHQIDDIDEELFQMLAQRMKLAQGIGEYKKRNNIAIVQPSRWNEIVEKATAKARLQ
                     GLSDHFVETVLRAIHEESIQQQTKVLHQMIDTQV"
     assembly_gap    595..623
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<624..1100)
                     /locus_tag="PK198_27610"
     CDS             complement(<624..1100)
                     /locus_tag="PK198_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRF42600.1"
                     /translation="MTMNFQPIFGNEHKRALLIAGPCSAETEEQVLQTARELAPQGID
                     LFRAGIWKPRTRPGEFEGIGTQGLAWMRRVKEETGLRTTTEVATTQHVFEALKYGIDV
                     LWIGARTTVNPFSVQEVADALAGVDIPVLIKNPVNPDLKLWMGAIERIYKAGIRRIA"
     gene            complement(1320..>1509)
                     /locus_tag="PK198_27615"
     CDS             complement(1320..>1509)
                     /locus_tag="PK198_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRF42601.1"
                     /translation="RLAKSSPDMWAPIFRQNRDNVLDVLDEHIEQLSRFRSLLIKRDF
                     DTFYRLMEEANEIRRIIS"
BASE COUNT          306 a          412 c          402 g          360 t
ORIGIN      
        1 aacactcctt acacctgcgt atcaatcatc tgatgcaaca ccttggtttg ctgttggata
       61 gactcctcgt gaatggcccg cagcaccgtt tccacaaaat gatcgctcaa gccctgcaag
      121 cgcgccttgg cagtagcctt ttccacaatt tcattccagc gcgagggctg cacgatggca
      181 atgttgttgc gctttttata ctcgccgatg ccctgagcca gcttcatgcg ctgtgccaac
      241 atctggaaaa gctcctcgtc tatgtcgtca atctggtggc ggaggtggtc aatgttttcc
      301 aaaaacgcag ggtcttcggc gcccgaaagg cggaagttga gccgttgcgt cagttcctga
      361 tattgtaccg gcgtgatttg ctgggcggcg tcgctccagg cctggtcggg cgtggggtgt
      421 acctcggtca tcaggccgtc gtagttcagg tcgagcgcct gctgggccac atcctgcaag
      481 gtatcgcggc gaccgcaaat atggctgttg tcgcaaataa tgggcaaatc cggaaactgc
      541 cgcttcaact cgatggcgat ctgccagcgc ggcacattgc ggtagcgggt ttcgnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnggcaatg cgccgaatgc cggctttgta aatgcgttcg
      661 atggcgccca tccagagttt gaggtcgggg ttcaccggat ttttgatgag caccggaatg
      721 tccacgccgg ccaatgcgtc ggccacttcc tgcacgctga acgggttgac ggtggtgcgc
      781 gcaccgatcc acaacacgtc tatgccgtat ttgagcgcct cgaaaacgtg ctgcgtagtg
      841 gctacttcgg tggtggtgcg caggccggtt tcttccttca cacggcgcat ccaggccaag
      901 ccctgcgtgc cgatgccttc aaattcgccg ggccgggtac gcggcttcca gatgccggcg
      961 cgaaacagat caatgccttg tggcgccagt tcgcgggcgg tttgcagcac ctgctcttct
     1021 gtttcagcgc tgcaagggcc ggcaatgagg agcgcgcgct tgtgctcgtt gccgaaaata
     1081 ggttggaaat tcatggtcat gatcgttggt atttatatgt tttacaaaaa aatcaaagat
     1141 ttcattttca aagagttatt tcggattcaa aatttcggat ttaagattcc tgattcaaaa
     1201 tttcggattc cgtctgacga taaactgcga accgcgcaag gcgcgggaag cagattccgg
     1261 attcaagatt cctcattgca ccgcccaccc cgcgccacag gcgcggctac cgctccctct
     1321 cacgaaataa tcctcctgat ctcattcgcc tcctccatca aacggtagaa ggtatcaaaa
     1381 tcccttttga tgagcagact gcgaaaacgc gatagttgct caatgtgttc gtccaatacg
     1441 tccagcacat tgtcgcggtt ttgcctgaaa atcggcgccc acatatccgg cgaactcttg
     1501 gccaagcgt
//
LOCUS       DAORLF010000674         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_5506
            NODE_42155_length_1500_cov_2.119031, whole genome shotgun sequence.
ACCESSION   DAORLF010000674 DAORLF010000000
VERSION     DAORLF010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867723
            Sequence Read Archive: SRR1068202
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:16:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,464
            CDSs (with protein)               :: 5,464
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 8 of 18
            Pseudo Genes (incomplete)         :: 7 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42155_length_1500_cov_2.119031"
                     /isolate="AMR_MDS_5506"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1119)
                     /locus_tag="PK198_27620"
     CDS             complement(<1..>1119)
                     /locus_tag="PK198_27620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42602.1"
                     /translation="PVGDLSGNSYTFIGVLMVADGDYISLTAGFSAEGGYTDHRVDRA
                     PGCGAVTTNIVVDGFIAPSDNGTYMLDGTRNNAPRWSNGSVELVYLGGYWGFVRLGQS
                     PYTLATLFTSTNSFVPCNGWFGFGLPNLSGACGIAPSSVPVSAGPSCAITNISLANAG
                     ACNDNGTITQTDDYFTANVTITFAYAPVGATLTLKQGSNVLASTQTDLTCTTTYTFEN
                     VQIPSNGAAVILTAEFSPNCTFTSGVLMTAPQPCSCQPTSAFTNCPVALPLNVAPGTC
                     SAIATYSPTANGNPAPVYTYSFSGATAGSGNGTGSGSTFAVGTTTVTITAVNDCGTAV
                     CTFNVTVTDNILPTIECFNQALAFNGEASFSLYPDELA"
     assembly_gap    1120..1210
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1211..>1500)
                     /locus_tag="PK198_27625"
     CDS             complement(<1211..>1500)
                     /locus_tag="PK198_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF42603.1"
                     /translation="TVEMAGSGDVIYIYDGPNGTGAFLGTLSAGSSPTTFTATASNST
                     GCLSAFFYAFSGQGAAGWEADFNCVRIYDITADNISGCDGANTTCMEDDTYT"
BASE COUNT          349 a          353 c          403 g          304 t
ORIGIN      
        1 tctgccagtt catcgggata gagcgaaaaa cttgcttccc cgttgaaggc caatgcctgg
       61 ttgaagcact ctatggtcgg caagatgtta tccgtcaccg tcacattaaa agtacatacc
      121 gccgtaccgc aatcattgac agcagtaatg gttacggtgg tggtacccac tgcaaatgtg
      181 ctgccgctgc ctgttccgtt tccgctgccg gcggtagccc cgctgaagct ataggtataa
      241 accggcgcgg ggttgccgtt ggctgttggc gagtaggtag cgatggctga gcatgtaccc
      301 ggcgcaacgt tgagcggcaa agcaacgggg caattggtga acgccgaggt cggttggcag
      361 gagcagggtt gcggagcggt catcaatacg ccggaggtga aggtgcagtt gggggagaat
      421 tctgcagtca gtatcacggc ggctccattg gaaggtatct gcacgttttc aaaagtgtaa
      481 gtagtcgtgc aagtcaaatc tgtctgggta gaggccaaaa cgttggaacc ttgtttcagt
      541 gtgagggtag cgccgaccgg agcatacgca aaggtaatgg tcacattggc tgtgaagtag
      601 tcgtcggttt gtgtaatggt gccgttgtca ttacaggcgc cggcgttggc caggctgata
      661 ttcgtaatgg cacaggaagg gcctgccgat acaggtacag atgagggcgc aatgccacag
      721 gccccggaca gattaggcaa accaaatcca aaccaaccat tgcaaggcac gaaagaattg
      781 gttgaggtga acagagtggc cagcgtgtag ggggattggc ccaatctcac aaatccccaa
      841 tagccgccga gataaacgag ttcaacactt ccgttgctcc agcgcggggc attgttgcga
      901 gttccatcca acatataagt accgttatcg gaaggagcaa taaaaccatc aacaacaata
      961 ttggtagtaa ctgcgccgca acctggcgcc cggtccaccc gatggtctgt gtaaccgcct
     1021 tctgccgaga aacccgcggt cagtgagata tagtcaccgt ctgccaccat gagtactccg
     1081 atgaaggtgt aggaattacc acttaaatct cctaccggan nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn cgtgtaggtg tcatcctcca tacaggttgt attcgcgcca tcgcagccgg
     1261 agatgttgtc ggcggtaata tcataaatgc ggacacaatt aaaatcggct tcccatccgg
     1321 cagcgccctg cccgctgaac gcatagaaaa acgcagacag acaccccgtc gaattgcttg
     1381 cagtagccgt aaatgtggtg ggcgaagaac ctgccgaaag cgtgccgaga aaagctccgg
     1441 tcccgtttgg accatcgtaa atgtatatta cgtctccgct gccggccatt tcaactgtta
//
